LOCUS       DAOQNN010000001        53340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_121_length_53340_cov_12.770348, whole genome shotgun sequence.
ACCESSION   DAOQNN010000001 DAOQNN010000000
VERSION     DAOQNN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 53340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53340
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121_length_53340_cov_12.770348"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..348
                     /locus_tag="PKV97_00005"
     CDS             148..348
                     /locus_tag="PKV97_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04285.1"
                     /translation="MLTEAEFAEYSKRLFACKTDGEVDALVTEIKGSGRNISKDMAGR
                     LRQAIETKRKSLEAQAKAAGQS"
     gene            353..805
                     /locus_tag="PKV97_00010"
     CDS             353..805
                     /locus_tag="PKV97_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04286.1"
                     /translation="MAATSSQWKVGDAAIYGKHGLGRIESVEPMISFGIKNCRIQISP
                     QQAQISLRRPCDEARAKDVLSIIGGPAPKPTAACWATRVRAYRDLINQNIPEEVAGLY
                     RELGCRQQLTASEKQIFQQARDLLVADLTPLYGAKALDKIKEAAGKAK"
     gene            802..1557
                     /locus_tag="PKV97_00015"
     CDS             802..1557
                     /locus_tag="PKV97_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04287.1"
                     /translation="MKPITIANADEWIAAGWHGAGNAPHDKKAEAGWRHAVNQALAHR
                     PQIIILGSEKAADDAAPRLHALGFEVWRCEGKGINGVYTYVGDQPENAEVRVIGEAAQ
                     LGQVRALGCEAAGEALEMTALQALPGIGPKTAAAILEDGFPEVADDWLPRCRGAEKVK
                     AVLAGYAQALEEARLVRVPIRKMEGPKDDLRALGLGRLADRIESEPHIEHDGDPCPAD
                     DFGDGPPVFDDDPAQFGGSDFYGDPFQGVGHGD"
     gene            1547..2473
                     /locus_tag="PKV97_00020"
     CDS             1547..2473
                     /locus_tag="PKV97_00020"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HNB04288.1"
                     /translation="MGTDTKVQWCHHTFNPWLGCDKCSEGCKNCYAEVSTPVRVARSK
                     GLELWGAQAQRRIASEEMWKQPLKWDRDAEKAGIRHRVFCASLADVFEDYRGPDADAV
                     RAARFRLITLIAQTPHLNWLLLTKRPENVAKLWPVTLPGIQHYDNIWIGCTVENQARA
                     TQRLPELLKIPAVIRFVSYEPALEAVDFSRWMWPVHSSWDSKYKTPEDALAAGSAVGR
                     HRQALVHADARFIDWVIVGGESGPGARRFDIGWARSVVKQCQEGGVACFFKQAGSKPF
                     IDDKPLSLRDRKGGDLDELPPDLRVREFPDAA"
     gene            2463..2936
                     /locus_tag="PKV97_00025"
     CDS             2463..2936
                     /locus_tag="PKV97_00025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNB04289.1"
                     /translation="MPRDPEKAKAWKRRYLQKQKIEKYGVEAADKDMRGRHGHHAKGE
                     RNGRWNSSGRLLTSHGYVAVRVDPEHPRAWGPSHGSQRYAYEHDLVAEEMIGRHLQPN
                     EIVHHRNGDKTNNRPENLAVETRSDHAREHTAVPGARDELGRFAPVNPRVRKMSP"
     gene            2933..3130
                     /locus_tag="PKV97_00030"
     CDS             2933..3130
                     /locus_tag="PKV97_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04290.1"
                     /translation="MSRNKKDGCGGHHQRLRAGPDIKQAAREGRRAAERQKLAELRAG
                     AEPDAVVMPKTDEVSNPRDWD"
     gene            3134..3637
                     /locus_tag="PKV97_00035"
     CDS             3134..3637
                     /locus_tag="PKV97_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04291.1"
                     /translation="MEIKTLDEAQGTTREMIAGYCERKGWTRRITATGTRQIPPNGSQ
                     SWSEEDPWFLVARIAEVEKRTEQEILREINPRLREFPGAAAQAAHHRGKWMCVDTSSG
                     SPVIVIGRFNRLDNFLHSEHLARGSFVGYGIEARDAWRQRDVLRFWPIDDFGSKVRWP
                     ERNGAML"
     gene            3634..4497
                     /locus_tag="PKV97_00040"
     CDS             3634..4497
                     /locus_tag="PKV97_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNB04292.1"
                     /translation="MSLEPIRIEDVLYWHLRNADCFEQLELLKDKCVDHVFDDPPYSE
                     HVHNNSRRGGSKGAPNASPATQDGTLIRPKRGARLAISVERPLEFEPLTDADRKVLAR
                     HYARITRRWVGVCCDIEGRQAWQDELEAAGLQYVRTLFWHKVGSTPQMTGDRPSEGME
                     AIVIAHPKGRKRWNGGGKDNFYSFPIVRGTHPDRMHPTQKPIELMRQIIEDFTDPGDL
                     VLDAHAGSCTTGVACIEKNRRFIGFEQKKLFFDKGYARLQRASRQEILFRVAPTGKQE
                     KLLLDVPGASV"
     gene            4494..5279
                     /locus_tag="PKV97_00045"
     CDS             4494..5279
                     /locus_tag="PKV97_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04293.1"
                     /translation="MNPEQYEREHQQVARFYLRELLSSATPEDLIAFGGELDGSRRDT
                     TREKVSYLAGAQLGLDARPGSRAWVRRGLHLRVAENRRDRALCAEIVKRRHYLGRWPA
                     PIKTLILSYLADLAGAGPGTAGAAALVMVACLPAQSHVTRALGLALYEVLTLVRTWRA
                     DDLGPQLAPNLTPATLRCVVKRLRADWVAKKCRAGGLEAAPRLLLTFADPVQGHDGAT
                     YTAAGATACGPAAGGKLAFCWALDDEIKERLRAWMVAREEKRS"
     gene            5276..5491
                     /locus_tag="PKV97_00050"
     CDS             5276..5491
                     /locus_tag="PKV97_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04294.1"
                     /translation="MIEFDTICPVCGLDADVQDEVSAGEEVDCKNCGMPLMVEEWDDG
                     TMSLVLPSDDEAEIEIKVADEPNPPHP"
     gene            5466..6053
                     /locus_tag="PKV97_00055"
     CDS             5466..6053
                     /locus_tag="PKV97_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04295.1"
                     /translation="MNPIPLTHKGRVYAYACGRCHLVHAGACEGHRHGVKTIAALAAH
                     FREQAEACCRCHECKAPLAGERWGNCPPCEGKRKTEFEAIKAKQRAEEEAKNESVKTA
                     TSAEAAAALAQHMSDISEVCWAAGWMEGTEYSLWEMLQGGPREWGMCRVSHADIGRLR
                     DLHEESGGWWIWGEQGQRFVTAAEWLKIYEGRPAC"
     gene            6047..6448
                     /locus_tag="PKV97_00060"
     CDS             6047..6448
                     /locus_tag="PKV97_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04296.1"
                     /translation="MLTEKQKWLNVLKHIAFPIVLIGGGLCFVIWLATYTPPDLRDPP
                     SKPRMPPAVKPIETDHFGYCMRRAFNQARASSYCAGMQEVCNRFVVDRAFGEGHYYDL
                     ELCLEFAAENCAPPYVLDRQQLLKECPEIKR"
     gene            6457..6831
                     /locus_tag="PKV97_00065"
     CDS             6457..6831
                     /locus_tag="PKV97_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04297.1"
                     /translation="MQTRTGVRGNCLSACFASLLGVSIEKVDFSCGDYTDGNWYDVAC
                     EKLRPFGLTLVLVDLRPEITIPAELYVIANGPSPRGHRHAVIYRGDELAHDPHPDGGG
                     ILSIEYLSFLLPLHLEVPCVPS"
     gene            6816..7514
                     /locus_tag="PKV97_00070"
     CDS             6816..7514
                     /locus_tag="PKV97_00070"
                     /inference="COORDINATES: protein motif:HMM:NF025717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HNB04298.1"
                     /translation="MRPKLIYIAGPYTPTEESSVEQNIAAARKVAEQVASPKIFPIVP
                     HNTGTAIEHIGDYQFWINGTIELMHRCDAVLAMPTWERSRGATAEVRDADRVHLPVFY
                     NIPDLIGWVSGYTRPKHGQIGQLADLLAGLPQSISLRNGDRTLEVRDWRKEADDGILY
                     RCHWPERSARIRLTSQFSTFEEVEVAEHVEPATKSPFPVGGGAREPATLRAIILPASV
                     DKVEFSIGFVDPPK"
     gene            7511..8122
                     /locus_tag="PKV97_00075"
     CDS             7511..8122
                     /locus_tag="PKV97_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04299.1"
                     /translation="MKTFDYRGSSAALHVNGSNVRIDWNGQTFTGTVKNNRVQIVPPH
                     PIAVEASAALGPSTTAVVRDVRPEAKVRHDVRKAIGALPGVYLMDNPVGHAEYYDDKT
                     NTIRHVDYGLEEGSPDLIVVLRRLVGCQMIGLELKAPGKSARANQTACHERWRRAGIF
                     VYTVQSGEEALLAIADARRRLSEDEPARPATAVQTLLPIESAL"
     gene            complement(8177..9634)
                     /locus_tag="PKV97_00080"
     CDS             complement(8177..9634)
                     /locus_tag="PKV97_00080"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNB04300.1"
                     /translation="MERPYQTRLFNDTLAALDAGFRRLVECMATGLGKTHTSIRQIQY
                     FLGLDYKVIFIVDLDFVVDDTAAKLQEHGIPVGIIQAGKKMQAGLSVYVCSLQTLIER
                     GLRPFQDEPRVVFILDECHIFAGPQTLDLLHAYPNAIHIGKTATPQRGDGTALGNFYE
                     KLIEGPQLGWGMTHGLCSCGAEVPINTCHNPEPYLVRRIVTHAPPKAQDKLAWDPVEA
                     WFQFAAGMRSFYFCKSAKHAEETAERFRQLGVGAEAVTAKSKTSLRKNFRARFRSYEI
                     LVVCTHSVGIKALDLPEIECVGVWRRISVPGIYQQMGGRGARGGKEKMVLLDGCGSLW
                     DLGPIEHPRVYSLEGDPIKLASGRAPSLTTCRACGAVQPREAACVRCGGDLEPETPKV
                     SKTNQLAEIKEVSLSTVQANQLRALIDRGMRAVMPSVLQRQLAKKARGEKIGRPIGPW
                     FAVEWAVTEFKKRHGITPTPDVINALKKEFSGGKD"
     gene            9693..11645
                     /locus_tag="PKV97_00085"
     CDS             9693..11645
                     /locus_tag="PKV97_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04301.1"
                     /translation="MTEPRGTQSIDIPDYAGLLDKYEALQRDRDDIHKNLVTVRVALA
                     DMESERHGLRAQVKMRDEEIAGLKQDMGADLVAALRRERAQLDTQLAGARKERAQFFA
                     DASRNATEREEQRTRAEKAEAELTKFVSLVRAWSAADFQYARAANCGESDPLQAAVEA
                     YNSAQAALLAAVGITEYDKLSAVISAGGCEHRKELDRLYAPKPCTGCNLTMPLESGKV
                     CDACKNSLPHALWLLFTATPMKERQLAGEAIWHILTARNRQNDDRTELRSEIEHLQRV
                     SKAAHDRAEKAEGAVDDMGNQIALVRDWWYRRSREWKRSARGFRTKAKMFHSALLLAH
                     KERDAVRAALDAFTRADTQPQIAGFLVANGEAVRFENGNVLPADPQTQIAHLRQINTE
                     LKQELARLTKPFPGSEFREIEARHKNIADSADLSESAHAAHKDRARLLDALRNIDAAL
                     NEVLKINQSLKRELAAEKSRQRNKEDTWGYQILVRSRRLERALDSSERKRAELAMRLV
                     EEQKKLSKVEERIERERASRRAAEGIAAIAKKDLEKTQAEIAAQKNDLADLELERQKA
                     VAESASRFDCHRGPGTTEPGCGGCVSCLLRIEEGMAAKVVRAEKERDAAITRLRETDL
                     LRRENIAEAQSLRARLAELTPQPPKE"
     gene            11648..11920
                     /locus_tag="PKV97_00090"
     CDS             11648..11920
                     /locus_tag="PKV97_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04302.1"
                     /translation="MSEQSKNVEHYRNSTDPQTPELRLYVERDEGLVMLYEADMTSRS
                     PTAQIVNLHDKIIMLAADARWLRDTLTSMDLEDGPDDAEPPLGEPS"
     gene            11917..12333
                     /locus_tag="PKV97_00095"
     CDS             11917..12333
                     /locus_tag="PKV97_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04303.1"
                     /translation="MSKIEIYGESDDIITIDGDYDDEVEAIDGRYLLFADGTVVSGEF
                     TEQGVWRFKVEKQGQATALHEPAEEFEDLGSDMVTLEYPGDLSLLWDGSEMPPDAPLE
                     DLLRAAKIRFDLIEACHFEDKDENALAVILKALQDK"
     gene            12512..12874
                     /locus_tag="PKV97_00100"
     CDS             12512..12874
                     /locus_tag="PKV97_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04304.1"
                     /translation="MTFLSIIAPEPPKEIAELLEMLYALGPFHDDLGKEGRGQIVLSI
                     LSFAQALPDTVQLDPIKTRELPMTERWQRKGVLQEKWRHEFEIKCNALLGAESPVRVL
                     ISPYCTKGTISIDLVNQC"
     gene            12937..13506
                     /locus_tag="PKV97_00105"
     CDS             12937..13506
                     /locus_tag="PKV97_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04305.1"
                     /translation="MFNRIALVFDAFGIAGANRSFGQTKLADSMGISPSILRRRASEA
                     RHGTAHRGMPLDQILRIAEIAVQELKPLQGEDAQVVVAWMLGLDRLIHFAIQNKLVKT
                     RGKNAMTLGFAPVVQRLERWVHEMDPRMKDIPLPEKQKIRPPAAGYVPSLDPSPRSIF
                     RPGMRRAQLDKKTLDQTRDKLLRKTKRAS"
     gene            13503..13640
                     /locus_tag="PKV97_00110"
     CDS             13503..13640
                     /locus_tag="PKV97_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04306.1"
                     /translation="MKTLFMIILVAPVVLFTPLQGCFLPPPPTPLNVVPVGKPASPGW
                     A"
     gene            13643..13975
                     /locus_tag="PKV97_00115"
     CDS             13643..13975
                     /locus_tag="PKV97_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04307.1"
                     /translation="MSLGIILKADWERFGEKFQEAKAERIPQLRDLIASGKENLVRLQ
                     KQLDELPGCIETWEAELRDLSRPVQTPPLSEILALVLGRSQVVPAEVPRPIGEDAPQK
                     ISAPGGEP"
     gene            13972..14154
                     /locus_tag="PKV97_00120"
     CDS             13972..14154
                     /locus_tag="PKV97_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04308.1"
                     /translation="MSRKANKRNIQTVLGRISTQCKEDPTLAAFWVETINNACDSAGD
                     AFGTEGQNDPRGDQRD"
     gene            14162..14233
                     /locus_tag="PKV97_00125"
     tRNA            14162..14233
                     /locus_tag="PKV97_00125"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14194..14196,aa:Asn,seq:gtt)
     gene            14238..14311
                     /locus_tag="PKV97_00130"
     tRNA            14238..14311
                     /locus_tag="PKV97_00130"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14271..14273,aa:Trp,seq:cca)
     gene            14504..14962
                     /locus_tag="PKV97_00135"
     CDS             14504..14962
                     /locus_tag="PKV97_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04309.1"
                     /translation="MSTEREIRLRITPASAAYCGTGRIDGTSCRHLRNTEDDAWKCGA
                     FSQLIPIGRGMPPRLPECIAAEKSKQPWTEQRPTEPGYYWLSFPADNRPTDAPPVFVV
                     SIDSSPTGRMSVTSCQIKPVMANVNLLDSYFDGTKWMPRTTPVDPFKESP"
     gene            14959..15342
                     /locus_tag="PKV97_00140"
     CDS             14959..15342
                     /locus_tag="PKV97_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04310.1"
                     /translation="MSTNCTHAKYIRGELRLPVAKLAEFQRLELPATNFLQIPSSYRD
                     AGDEVVIVKPWWCGDWSGNSFDTLRDTILPATRGSADLVFIWEGGEGFTGLRVEDGKV
                     TEHDVEFPSVPRALLRSSSGLLLPR"
     gene            15299..15661
                     /locus_tag="PKV97_00145"
     CDS             15299..15661
                     /locus_tag="PKV97_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04311.1"
                     /translation="MLFFAAVAAYYFPGDRYDTGQVACPKVPAALRAARGFAHRSAPC
                     GSKALICYKLRCAVGVVADRGPYGILGAQGWRWGTRLGPKERWAGEFDLWHGLAAELD
                     FRGKEPVQYTWLKKESTR"
     gene            15658..17052
                     /locus_tag="PKV97_00150"
     CDS             15658..17052
                     /locus_tag="PKV97_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04312.1"
                     /translation="MNPIPPVEDFEPIDPPAPTAPLTPPAPPELRIVPPPAEEVTAAE
                     GPPPAESPPAPAEAAPSSDHNPLLEALAQGPLVMDVPRTASEAFHFLSVRAAEKVIEE
                     AKREGGAAAAQMWGNISETYKGLSAFSAQLPQAKARHLKWSYLVILEAIPDSAVELGQ
                     LLRWLEKVPACADDKAVPAAFLWFHLGQCSFIHGQSLPRPQRTAQGWTCEHNGLTRVL
                     SNEESYGLQQQRHFWSQRYYDRCMQTAGILLRKLSGIDVKFDPNKNPLEVVCMKCKAT
                     VRAGSSHDCPAESAVPTDGVPPMRAFTRPEAPAEPSPAAFDDAPAAEAAADPAAPANP
                     TEGTPVESPIEAARKRVEELRQERDEARGQDSYDHIDELFENAQEELNELLAEAEDSG
                     ETAAAAPAGDGTAAATEAPSPVEAIEAAFATAIVPADPDVPTMIGNKVPAPAAVPSGT
                     DEEPTAFATAPRED"
     gene            complement(17308..18285)
                     /locus_tag="PKV97_00155"
     CDS             complement(17308..18285)
                     /locus_tag="PKV97_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04313.1"
                     /translation="MAYQVPSASAPHANIVAAINGNHASVDSTGKSHWDSTEIDPSGT
                     ELYISVPDATDFLTGVAVVNAAKSLYNTHRTYSNAGINTHIYAHLNSDTTNVVTTADM
                     DGTSLYNTMFQAIKTLYLEIRTAYVNHINNKKPDQTTAAYHTDIDTVDVLGAVPTIND
                     EADLAVDMNNCKAQYNLHIVNTTGVHGASDAGNAITAANAVQGNLDTFITLVNQMKTK
                     INAHFATAGMVHTGAFGADNQNTIVGAAVSYPAGLFTLINEIYTDYEAHRADATLYHQ
                     VADATNVLTGGYYPVTTLAGFVTAAQEQKTKINAHFRNAPAYSRAVRVV"
     gene            complement(18296..19129)
                     /locus_tag="PKV97_00160"
     CDS             complement(18296..19129)
                     /locus_tag="PKV97_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04314.1"
                     /translation="MTTYAGLDTYPADATLPDPNQPAVAPTVSDPEKVVLDRTTFLLK
                     RLAAHAESQSPLRYRCTDGTKIVFEPVFGVVSFDTMPRYMQIATATDITVANLDGGGS
                     LIGNNTYYIYLSWIAGAPKFVISLSPPDPYRLYKDGSTSHALVGVVQTDATAKFMKGV
                     GGRHEFLYLEPPQVQSSKATTTGDTINLSTRLPPIADTAILQVNGVSTVAVIAALRPF
                     VAIGPEGFPMWRELVLSTGLVDARVQDTVKIPLLPGKKASILTNSVTSFIDVNLAGFT
                     F"
     gene            complement(19126..19971)
                     /locus_tag="PKV97_00165"
     CDS             complement(19126..19971)
                     /locus_tag="PKV97_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04315.1"
                     /translation="MATQYPGTEADVTEITIPDDNDVADAESVNRAFRALRDSIKLGL
                     ATQNDQPLNIRSYLRSTDFAKIDISPLSTWIQQIRAFLPYRYAQVAWNADSFTVLDVD
                     TGGASFDASTVYYCYLGWDNGSSTFKRKISKNIPDEKLVFQKVDSFLKYVGSFHTDSF
                     KRVVPYSKEGDYYTIDNPFPVLSTSGTTLVTANLSSFLPTYARKVRLRIKMINTSTAA
                     TDKLLISAVGAPDFQEIVIGKGADVQQMVTIDLPLRTNREFQAKMDAGGSAIGIISLE
                     GWWER"
     gene            complement(19980..21608)
                     /locus_tag="PKV97_00170"
     CDS             complement(19980..21608)
                     /locus_tag="PKV97_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04316.1"
                     /translation="MPLFIDYLKRFLSWHYFEGAGEAFQAVVTKPIDQLIAAAKEAVV
                     ARFYRYAPDGALPYLLQNYNLDWVERFIPAQRRQQVADAWDTWEKAGTEEGIIAEVQR
                     LGYQTVGMLPVWLYKHSSDGITDTLQFQPSLQPDSNPLFHPTKLTAPRDIATEGEWGA
                     REWWIDLNFHASSFFVVIHDPPFAFRRWGDPVPKWGAPARWDALVTGDPVALRRIYTT
                     IKKFTGAEWSCRGVVFSYSGRYKLAPAQLTGVAVRTTFDAWACGLAGYITRWDGTKWT
                     QAVSGTTANLAGVFIKPDTETAYIVGQAGTILRWTGTAWVAETSGILTNLSGFAYAGL
                     ERWACGQGGVILFDVGFGWTPSTSGVATDLRRMLAFSRTDIWAIGVGGVALHWDGASW
                     TSTTTGVTGDLNGIWGRAPNDVWAVGAGGTIIHWNGTAWSTVSSPTAAGLFSVYGLPD
                     GRAVATGQGGIVLAYNNIVWALAVGNLNGESADTVHGTIADNFWAVTQQGTAYHLTAA
                     GVVYAGAVWNGFTWDDGTKYTLNYVIKWRREKWE"
     gene            complement(21611..22774)
                     /locus_tag="PKV97_00175"
     CDS             complement(21611..22774)
                     /locus_tag="PKV97_00175"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HNB04317.1"
                     /translation="MSLSFEELQAGKDSAVIFDEMITDLKGITAANGTRVFPVDDWSD
                     GSFPRTLLEGWSITLSRAYKNIPAITNGGFILLAPDSWIPTIARNFYRVEPFVATYTT
                     GKLKYTVAAGNGPYSIQGGRSIASTEDRLRFFSTNTTPISISSAAPVWIPIRAESPGA
                     KYNVLERQINQLNTSLPGVTVENVPEGTDLSWITNYGTDAESPAEIKARCLRRFGRLS
                     KLQQYPEEGYISLVTDLVPQVKKVTVFTNYFQGTARPLCATLFLAGDSGAVAPAVVAQ
                     VLAAVRPYRCPLGTIFADSCTVQNQPVRGTVELLPDYDETVARSAIAAQLLDYQKAAQ
                     IGDRIVAAEVYERVMTPAGVKNYKPNGLFDFVLGANQVVNFQPSLDFITWKPR"
     gene            complement(22758..23156)
                     /locus_tag="PKV97_00180"
     CDS             complement(22758..23156)
                     /locus_tag="PKV97_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04318.1"
                     /translation="MADNYGSDILLGATGIDPRFPIITGGAVVAYNIFRRVTTKETLP
                     AYHGNSIDLAERAGNKLDPKGFPSLERDIIRVCVFEERVSTVRPTVKLDTKKDQLNVR
                     ILIVLVTGETFTLVMDLSTVNAQVLNVAVL"
     gene            complement(23193..23684)
                     /locus_tag="PKV97_00185"
     CDS             complement(23193..23684)
                     /locus_tag="PKV97_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04319.1"
                     /translation="MDPVVKSGSRIEVRFDAVKLTGTDGSESLSRSDPYFYDTTLNHR
                     CEIYEWSRFGDRCVPTNDNQPISVYPAYTDSTCSTRMISIKDNPSATTAEVFGWLDRE
                     LYQADFEITTTATTFFMKDPLGRCTSGPLSSIGPTWRFFAAKTPALMFVPFTRTVSVV
                     KAP"
     gene            complement(23947..24177)
                     /locus_tag="PKV97_00190"
     CDS             complement(23947..24177)
                     /locus_tag="PKV97_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04320.1"
                     /translation="MKTRDALGRTWLVSLAADGSIDIAPIGHTTSPEALSREEEDRMA
                     DELEAELAAAGPSEVDPFERAHDAWKESRLAA"
     gene            24241..24741
                     /locus_tag="PKV97_00195"
     CDS             24241..24741
                     /locus_tag="PKV97_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04321.1"
                     /translation="MSLLSKKLAQLLDKLLARIDYLASYECKITKVNADGTVDLDPFD
                     PRLKPGPQNVELEVPAGFEKVTPKTGSLCMLSFKNGKQDAPFVGFFKLGATFSEIALR
                     ADKIKLNGGGAAVARKGDSVGQLTGKTAPGGGAVTFTYTRTGQPPVESPTVALAIDGG
                     NDTVEA"
     gene            24751..25020
                     /locus_tag="PKV97_00200"
     CDS             24751..25020
                     /locus_tag="PKV97_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04322.1"
                     /translation="MLSNLAFLVAEHATRVMSEPGSPTVEQITALNKAVNYLETANGI
                     FRQLPSDFEGMEGAGEAAVTARAVISQWTPSAPVASLPESAHPDR"
     gene            complement(25180..25497)
                     /locus_tag="PKV97_00205"
     CDS             complement(25180..25497)
                     /locus_tag="PKV97_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04323.1"
                     /translation="MKARFAAWKFWVLSLLLPGCAPFIDCMKLPVQTAIPKVVPALRS
                     SDWKPATDTLIATLGDVGLCAILAIAKTAGASIQEGEARPRVLSVEQQHAVEYLTIRR
                     ISR"
     gene            complement(25494..25997)
                     /locus_tag="PKV97_00210"
     CDS             complement(25494..25997)
                     /locus_tag="PKV97_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04324.1"
                     /translation="MRAGALAALLLSGCTYMLDPRFFDLGATDAGDLSGSADTDGAAA
                     CDPPLADIWAGTAPNPSTVELQPCVAVFHLQNSSPATLMRKLTTMPGKALTASGAFSH
                     EAKCNGWFSVGFSDDMGAFSTVSGAAGSIVALGTARRGQTSFVLTVQVSQPCDVYLTK
                     PVGEVAQ"
     gene            complement(25994..26278)
                     /locus_tag="PKV97_00215"
     CDS             complement(25994..26278)
                     /locus_tag="PKV97_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04325.1"
                     /translation="MPVYLGKQAETKVGEKLILHAEFTTKCLSTGTLALSFIDDDGTF
                     GKVLGQVRSGILDTDAIARKPQTKYVIAALSDLNCDVTIENVISITQTVQ"
     gene            complement(26529..26942)
                     /locus_tag="PKV97_00220"
     CDS             complement(26529..26942)
                     /locus_tag="PKV97_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04326.1"
                     /translation="MREILQQLLGNLPSILVFGAVLARIWDRHKQRQAIQAAEKNPEL
                     RKQLTDSLPPINVLIVIAVGVGLIAQIIAWRVQDTLAQRPQQDVQPAKCIDCRDDCSC
                     KNGQCKCGARPVPTSSLARAIPRYAGPIPSISLDL"
     gene            complement(26975..27658)
                     /locus_tag="PKV97_00225"
     CDS             complement(26975..27658)
                     /locus_tag="PKV97_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04327.1"
                     /translation="MAAEFTLGDRALIEGRLIWPESGAIRGSFEIDGEDPISGAVTLG
                     LPGVDIAVTIVSGGLSDTRVYANFNSGAGTLSKFPVEGQHFYQATPRTIFEQILNGSG
                     ETLFPEMPAICDQAIAHWMRIATTADNALAQLVRHLGLAYWFEPDGRVRIGQIDYPEV
                     TLDTLDVLPLDRPEWKRLDFADDDAIVQPRTTLVTDYGSYRVVEVRYYLARGIWRGEI
                     FYEDPATVL"
     gene            complement(27661..28260)
                     /locus_tag="PKV97_00230"
     CDS             complement(27661..28260)
                     /locus_tag="PKV97_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04328.1"
                     /translation="MSLLFWSGEIGGVIDKDELTPWNVLRLDGVMFPSDEAPFLLKGI
                     SGHVGFDIDRRKRKNRSGQKKTSTGSKSPKWTMVFMFWTPEQYQDWQSILQQINPKLD
                     ANRIKSRQLYHPLLADFEITQAIIYQIDMPVIDPQSLFAEVTVHFEEVNPPTKKDAKK
                     DLVTTPEVAIDKDFVSGEAAIPTPAGVPNSFDEGPPPEP"
     gene            complement(28257..30467)
                     /locus_tag="PKV97_00235"
     CDS             complement(28257..30467)
                     /locus_tag="PKV97_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04329.1"
                     /translation="MLAMGAPSGTYEQHVFGLELRDKFLGPLAKVVRVLEQGAAAWDK
                     FESRVRGGGMDKAMAKVNGLIARYEHLEKVVNRIQAPGAGRGGSGGGGRIGGGSSVLD
                     SHVERRKYEVWWDQQLRKQDAAAQRATERETKAAQRVAAQEARAIQRKEEAAARAAQR
                     ASKQSVAEMKPFADMWAKIGTKMYVDQLKEAKRKSEEIKKDFEKIGQGIVSALGYLKQ
                     GLVSIVGSSFEYGKKVVGTLVDAAKERGLLMTALEIQLGSRGKASSQLNKTIDIAQLT
                     PASNLQVIDLTKRMLGQQFTGRRLDAARAAWADVQAFGGDKAARQIEFWMSRTAARGE
                     ASTAGVSGIAKAGISERFIREEMAKQLGKSVGYKEGGPYTDKIDKAVRDAVGQRKVSS
                     GVFEVAVEKAVLRTLKQTELGAYSKKKGIESLAGVMSNIEEAVPSFLMRLNIDEFKGI
                     KELKRFLTDILSFFNLGTKEGQRMAQIVEDLTNTLFGGLSNITKADMARFFEDAAVAA
                     QELVKVIQRAWELVGELIHDTSGKGLGGALEKAGQVFGTAVGEALAAMLPSIVGGFAK
                     ALPGAVKGLAVGAGQAAWSLGTDLGLGVQGWFKKSVLGSDEGNYSKERFMKHARKSPI
                     ESASAGPTMSEYGGMSSAPAPPNLTAGQADALSALGVPSFDKGGVVPGPFGSPQLAVV
                     HGGEKFLGLGRGGDGGNVYIETVVIQCDETALKKNFTEWFLEELDDELAAGAPS"
     gene            complement(30675..31109)
                     /locus_tag="PKV97_00240"
     CDS             complement(30675..31109)
                     /locus_tag="PKV97_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04330.1"
                     /translation="MNDPTPTESPTSDTSKYEADVQAAIEECRTHLVNKGFDPPSVFV
                     IEALEMTIVAMPPSVEASDRLTRMISKKENADIQGYVTTFAAETVFWVKGQPTPGPAG
                     AIKYLKEVRAKSWLKANLFGVIAEQVQDQMEALRGNAQTKKL"
     gene            complement(31162..31629)
                     /locus_tag="PKV97_00245"
     CDS             complement(31162..31629)
                     /locus_tag="PKV97_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04331.1"
                     /translation="MAFNQIDRDDPRYDPSSLVVKVDGDEIEWLAISYGDDTDIQLAR
                     AGGTPFVVGDGPGAYMPQGGSLRMLKDKAAAYMKQLYDDAIAQGAIRVSAVRHTITVS
                     YAEWNMGTLTDTIIAVRFKGMQPGGQEGGTSALSTVEIPLIFSRIKWDGDVQM"
     gene            complement(31632..33101)
                     /locus_tag="PKV97_00250"
     CDS             complement(31632..33101)
                     /locus_tag="PKV97_00250"
                     /inference="COORDINATES: protein motif:HMM:NF022218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 family protein"
                     /protein_id="HNB04332.1"
                     /translation="MLVTVTFLDGQFGLLSDAPRACVTIGSASAGPLNQLTSITSKQA
                     LLDTFTAGPLVEADAFQLANGTGTGYAMRCGTTTPGVISTVTATLKAGSDAVLAVTGT
                     PVDGFRSILVRINSGGNVAGPTSPTAQISWDGGINYEPEFTIPVGGVYVPTNPVSGLT
                     FTFTGATIKTGDAFSLNVTAPVFGSADVVTVLEALRIDETSDWEFFHLVGPCSATIAT
                     AVQTELNLMKADGRFAWALVEARDQSTGETEAAWMTSLKADYLNFVAPQGQVVVVAGY
                     GYLLSAVSGRTYWRPLATTVAAHVTNTPTYTDHLGATSLGALPGIVTGPDGRTYVSHD
                     ERKVPGLAQARFLTIATIVGKRGFFVGDPATYSPATMAQSLSDYRKLMFTRVAMRTAN
                     LAMIIGTNLLGDKVEVNRNGKINGRFAKETESLMRAQLRNYLVPRDLIDIDVAIDRNE
                     NMKFTSRVPVTLLMNSYAYAEKVAFTLGFLNPNLVAVTG"
     gene            complement(33104..33409)
                     /locus_tag="PKV97_00255"
     CDS             complement(33104..33409)
                     /locus_tag="PKV97_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04333.1"
                     /translation="MTRETAQEWHNQDVAEEELIRGKQLLLVWHETALFHNSWKPDTG
                     LTREEYDAGIARAKAHVPGHVEPRIPPPSTIRLPKPPPAPVPTPPDDPPPATTGEVK"
     gene            complement(33406..34026)
                     /locus_tag="PKV97_00260"
     CDS             complement(33406..34026)
                     /locus_tag="PKV97_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04334.1"
                     /translation="MTFDTNRQINSLTEVLDELEKTIRYALQNPGFVEGLGPDEPPPI
                     PAAPVTDGTDVQFFLGEVENFAHMSRPYLVWTLGDTRQAGGQHRGTPLPNLQNPRPFK
                     KALDLIQCTMYGASIGQPCQGNDEPWKRGTVRGCEILRWCYWWALREHWGGNVTFERE
                     NWIEVSGNNQTGYAWASSFRVPMSQMRPAIPQHENQTVVPTMQRQP"
     gene            34171..34359
                     /locus_tag="PKV97_00265"
     CDS             34171..34359
                     /locus_tag="PKV97_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04335.1"
                     /translation="MHECWLESSEQRNRERAAMQMFPLSVRLAELRGEPLSRGELVAI
                     HTYPGMGGVPNREEMWPS"
     gene            34347..34661
                     /locus_tag="PKV97_00270"
     CDS             34347..34661
                     /locus_tag="PKV97_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04336.1"
                     /translation="MAVVIERWLRLDDRRLRNTTKSDSTCEARTFGLNGQPWPRITVV
                     VRMDTYGTVGYRVSWSRKTGPGEWWYEHDPMPRDILVELPEMIIALLDKTAALSETNA
                     QE"
     gene            34665..36305
                     /locus_tag="PKV97_00275"
     CDS             34665..36305
                     /locus_tag="PKV97_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04337.1"
                     /translation="MTTVMHTTAAPAPTHDNSDDGYPQLLLGLREAFSETVASRPPLF
                     GVNTEGLFDVFLSHLPEAARQHYSCRTCRKFVERFGGIVTVDDQGVTRSVFWNLPNIS
                     VSAFFIGPLFQMARFVETREIEGVFLSQDPEWGQTTSGKNEAGEDYHHFAVTPPPSLL
                     FRHPIDSADQAAANVREELGMLERGLAEFSRETVRTTETYLSSGDLYRSEKCIEIARW
                     LVKQHEIWGLKNARLRKNLLWVSAATAPAGWCHVKQSMIGSLLEDVKAGLSFDVLKRR
                     FNEKMDPLQYQRPQAPPSEQNIAQAEKVVAQLGAQGGLARRYARLDDVKALWKPRAAA
                     APAQDSGVFSHLKKPKAAQLVALPQRTFTWVKFRDQILPNATKLEMLTPRGAAQFCAL
                     VTAVNPDAPPLLQWDYPDARNPVSWYFIGNGSLASSWNLRPNTYVLVTAITRKPSMWN
                     EDRPATNHGDGVIFLLEGAKPTHHQRGGGMFVEQMRSEFHGVRRTLEAHFLSTPIADA
                     ECASACGLSLAAASQKTEIVIRYETPTETGTILIDRWD"
     gene            complement(36451..36876)
                     /locus_tag="PKV97_00280"
     CDS             complement(36451..36876)
                     /locus_tag="PKV97_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04338.1"
                     /translation="MISNNNPFRWLLERVKPLEQLPEKFLDGSALLAKGLAEKGFEQQ
                     TDPYGQWWAPTVTGRSFDQRDGLKNALRVSPYQLRNLSLLQLNHYAYQFHQRGSVYVW
                     GSIPARLMVPVVSRGLGRWAPDFHELARRIFERLVMTPR"
     gene            complement(36866..38164)
                     /locus_tag="PKV97_00285"
     CDS             complement(36866..38164)
                     /locus_tag="PKV97_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04339.1"
                     /translation="MRLRPSDFDPGLISDVTHTGTGQGTIRVTDLPYDGYVVELRCTR
                     SGDAVPSTSIVSTTSAAGVLKVSGTPRNNYTVRVQIMSTGYFRYSFDLGATWSATIAI
                     PASFEYQLAGTGLTLLFTGTMAIGDVFNFSTVGPAQFAATTYVVSRPILSCGTGTITP
                     SGQVTRPYRIAVAISGTGGFGEAQFYYSVNGGAPAGPIPLASSYTIPCVGITLTFDPG
                     CGPTYFGDGDVWQFSTRGSYTFDLLEGATAQELAAPCATTRPIVLNRPEPTGLRLLFE
                     AQSASSPNYVSGDFYGFSTEPPPDILEACVAGSDELLAPIRARYQKQQIVAWDNSVTI
                     NAARISAESLYDRKGQNWKEDRDSFAKNAARARAWGEQVGKKLMHPQITAFPLPGVLA
                     PRVVFGPDLEGVSEGPFGTTYESRTRYAWGSGIVTPPDDF"
     gene            complement(38315..38959)
                     /locus_tag="PKV97_00290"
     CDS             complement(38315..38959)
                     /locus_tag="PKV97_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04340.1"
                     /translation="MSRYRMVNVDSVALEAGHVLISGTANVSLFTTNGAAKLTGPTVG
                     IAASPVSALGKLQVIAAMGCGAFSITLYGAAGQRETLTYSAGGGTKTTMLDFSRLSMV
                     ETSAAPGAQVQILMVCRGVKRGGDNARGVVGVAVTPAAPNEPVQFVKDDFANVQCGAI
                     TPNFGDRLVGATGGLAKLRGMGDTGDDVGTADATATTSSGGKTYLRIAVDPGPA"
     gene            complement(38956..40071)
                     /locus_tag="PKV97_00295"
     CDS             complement(38956..40071)
                     /locus_tag="PKV97_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04341.1"
                     /translation="MIIDPIFGGPFPTDDPQTMGAPMARLIPAGYVEVGVDLTTKING
                     EVNLYLLMWDDDAGWLPLERLVANQRGVAPGPPLTGKNHFQGIQLPTQRYLCLYCPDL
                     TSDQITMAVINGYSGGIPLFNTFWTPWTATDPGPSEQANPENQEFAGSELNSGWDFTE
                     TWVEGGVNRFGNAYSAPPNQVFYSVGERPSWILMQGAAGIGPGFATISRQYASSSPLN
                     WYMSALCSFDVNNFPLGTGDVAIYFMSQSSAGAFDPNNWVGIYCGTDGSGKWGFSMAA
                     TIGGVFGGVFAGVPGSLYVCPGVAPDISKLVIHKVGNAYKAMVGTLGGSFLLLGTETY
                     LGFNPDRFGFAVANTNTPNLIVGVDWVRHEEKQVVNE"
     gene            complement(40102..41004)
                     /locus_tag="PKV97_00300"
     CDS             complement(40102..41004)
                     /locus_tag="PKV97_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04342.1"
                     /translation="MGSVTVQFGTTYPTSTPGALGSGIAPFLASPKGEALITLSSSVN
                     GFVDIWLLRWDGVSAWFPVAASAPPQPAPINAPQPFPFEPARRAQPPLNGQSRYVFDI
                     EKPGYYLLYAPNSRTADVTAAQLNELYPQSGAFSSGAQVRGALPTYAAPVAADTTGIH
                     AAVAANAANTFPGPIGTIESWGRNLRVTFAASWDGGDVIVDGTDQFGRAVSETWASAA
                     GSTVAGVKVFRTVTGIRKTAVGATANTASVGIGTAIGVPFSFTAGQEFLNGVAELATI
                     NTTYYHFIPGTAANGALNYNFIGW"
     gene            complement(41016..41726)
                     /locus_tag="PKV97_00305"
     CDS             complement(41016..41726)
                     /locus_tag="PKV97_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04343.1"
                     /translation="MSGKIIPLSAVTVTNALAGNDAGSAFTLTGNPLLGPQPLSVLFA
                     SGWDGGIVTITGTAVDPKNPRNPDGTPKLEARIETVTPPAGLPAIVETVWPFATVSAV
                     AKSAVGAAAATAQVKTLYPTQAPQLVGFRQNGIESQSIGGNIYGAMPLHANTLILEGA
                     VTSGTIELQPIFYENGRWWPLTTSSAVSIADTMLGKSNVGKFLTVADPSTVYHVWLTN
                     TGTFDVAYLYGKNGPSIS"
     gene            complement(41755..42858)
                     /locus_tag="PKV97_00310"
     CDS             complement(41755..42858)
                     /locus_tag="PKV97_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04344.1"
                     /translation="MKLGTRIYSQKKSPLAEAASRRGRGNSADAIAQLRVMAERGSAA
                     AKEALSMCASGIITPAAVPGTTDPQAMFESWKTDYMSAYQGASPVSLESGLAQKFTGA
                     SKKSHLPVPPSGLGPVEELVDGEPQGAQSPQGYDVPFEHVTRGPQGLIINRSDMASDR
                     VGMTTANVIAKGNDAAMMADFRVAEALNNGEVQLSFDRRPAFANNHIQNPVNPAIGPN
                     FVNLFNEKLDVPGLVTIVNAMSGYRNESNLPIVSGEIQFGIIVPPDLRGDLSYLLDRD
                     RDQFGATNWAHIYDFVGIVLPTLTNPKVYYVCILNGPVAPLYYSVHIPMEHIFRGPGT
                     EVYDEKRQFKFISREDNACLIADWRMIAKSRIP"
     gene            complement(42871..43281)
                     /locus_tag="PKV97_00315"
     CDS             complement(42871..43281)
                     /locus_tag="PKV97_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04345.1"
                     /translation="MAVRNYQRAGNTIVKRAPAKGSVAFAMGEILMINTSGYVIRGTA
                     TTGLLPFGVCANPLDTTGAADGDLQVQGEYGGYYVEVDNSTGADAILATDQNLPVYTL
                     DANSVSRLSAGKSKLGIFKGFSASGRVRVYIEPN"
     gene            complement(43296..45017)
                     /locus_tag="PKV97_00320"
     CDS             complement(43296..45017)
                     /locus_tag="PKV97_00320"
                     /inference="COORDINATES: protein motif:HMM:NF021627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HNB04346.1"
                     /translation="MSDIRERYKVSNPDWASAYCLASLGDTTTPPRERLIFPKGDFKT
                     TKGTFVFDDEAARMVMEDWNEWTGGLPDKGAADYEHDQSKDYLPGQDKLDSASFDLEV
                     VKGALWATNIKWTPLATKIIANREKRFTSPWWLYERSSKRIIRFINFGLVSLPATLGQ
                     KELMAASNAPTSASVQVPGDAFAGYYESSHGTPDRTVQTAVASQDPPPPAPAEPLAAV
                     AASTSSSEPPPVPALAAAPEVTTQEKAATMAYKDPNFVATRHGYALRYMASMAMDEMM
                     CAIHLNGEVPMLASAMNLSSVCASQAELVTHCAGAMAECMTDADADMDDDTEVDASLA
                     SVIAKLPDEAKTKVEAALASCKASKIGGLVQKAHKAALAAAGTERFGKGAMTALASLT
                     AAKTVLSAVATITGEQDPIRAAAALAAMQQDNPALRERAEAAEKATADATAAKDAAEK
                     KAESLAYEQVMTANKAKVPAASEEAWCRANIKTADALDAYFKAKKTPAIATNHEQPAT
                     TGTAAATTAAATSASPEQAAKDRDAIIASVEPDPKDLAGWASTGGGDERLKNLKEKKA
                     RKLGLIA"
     gene            45099..45665
                     /locus_tag="PKV97_00325"
     CDS             45099..45665
                     /locus_tag="PKV97_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04347.1"
                     /translation="MQGLLKRLATALHSHWFVLDHSDENFYYYECRCGQRSFAYLPGS
                     QGDPDFAWVQGLKRAEYAQLSPNARKSRARREILAEVQPHIARGNALHRRAQRAESRI
                     QGMLRKLMELPQPADRVWVVWDRGAEKTGNTNVLSTRRVFFKASEAGDYARAIGWEGA
                     VPDLLGSESAFMGGEPLINVRVICLPVK"
     gene            45647..45865
                     /locus_tag="PKV97_00330"
     CDS             45647..45865
                     /locus_tag="PKV97_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04348.1"
                     /translation="MPAGKIKLYVWQPEGHGPSSYFVMAASLEEAIKAVRKVAGPPSK
                     KTAYQNGAFYDGENILPPKIYLPGEVDQ"
     gene            45862..46152
                     /locus_tag="PKV97_00335"
     CDS             45862..46152
                     /locus_tag="PKV97_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04349.1"
                     /translation="MTTTDAKAPIWFRRLSRKLLRHADLYKPSRPRILQVDISATELR
                     IKVWRGIMIELEFLEPHEHNVTYEVTVPIFDEKTQSKAVKIYEAVKKKMRLK"
     gene            46149..46550
                     /locus_tag="PKV97_00340"
     CDS             46149..46550
                     /locus_tag="PKV97_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HNB04350.1"
                     /translation="MKKPHRPVYIRCALHVLRVPEIRQLLHAEQEHQYVLGLDVNLRL
                     IVVHLVCVGSVDQAQGHARDIFRELIRHNCHSFVLVHNHPYGDVVSSKGDRAVTAWLR
                     QCGEWLGIPLQAHVVVANGKQPREVGMPRKS"
     gene            46552..46845
                     /locus_tag="PKV97_00345"
     CDS             46552..46845
                     /locus_tag="PKV97_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04351.1"
                     /translation="MADNPEIISITVDDLAKMMEQATFRGITQAMTQHLPRNTELLPR
                     RHSENHNLRRKTKKRKIIARKLIDDFEPIQSTQQQSTISGLQEISMPKWKRVR"
     gene            complement(46823..47419)
                     /locus_tag="PKV97_00350"
     CDS             complement(46823..47419)
                     /locus_tag="PKV97_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04352.1"
                     /translation="MRSRKKVRVTKDDRTRAAYAQLAMAADALDLPDDDRPFEERRDE
                     LVEKLLSGWGKTGHRTAQVYTENVNQAFLRATVRVLDTPEVKAIFPYWRFLATLDQRT
                     SQFCDALANVVRPAGEAWWYDGRIPPLHMGGCRSILEPLTMKEAHKIGVTQYYPLVAG
                     DIHHGYGYANKSFQPDLSKFPPELVQIYVVAVNERASI"
     gene            complement(47406..48842)
                     /locus_tag="PKV97_00355"
     CDS             complement(47406..48842)
                     /locus_tag="PKV97_00355"
                     /inference="COORDINATES: protein motif:HMM:NF017850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HNB04353.1"
                     /translation="MTQWEPLLPPDVWTWGSITSANMTHEAALFQQSARLCDTLFRDD
                     HTAGLLQTRLLSIRGLKFSFLDAADQSLVVDDPELYRDWAYMFPRSVQLEAAFWTILE
                     GFCIGQNRWDMERRRSKLQIWHPGNCYWDKSRLCWRVYTREAGVIDLVGDLAPGKGKW
                     VLFKTWIAERPWYGGLIRAIGLLLLIRQVALPDWLKYEKKFTGSNVLTVPGMAAEIED
                     VQKTIDAVAKLTTGSTINLLEGMKLEILEPKSNAWEVFEKLLRYVDDALTILILGAVD
                     IVQGGEHGSKARAVVQDRPRQDRLENDVDILATVSREQILYPYYVYNRGIEDLEEVPF
                     PYWDCTPPEDAVRNAEIRSKNAAAAKSAAEAIEKLLVVYPDLDVDEFARQHQIPRRGG
                     GAASASIVERHGRVLRRSKPEAAIPGLRAFAKAVAGARTPEELRDRVMATYTKLGPEK
                     LAKQLAISRLEAEQQGIAGVKDKVRAQP"
     gene            complement(48936..49319)
                     /locus_tag="PKV97_00360"
     CDS             complement(48936..49319)
                     /locus_tag="PKV97_00360"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNB04354.1"
                     /translation="MILHTARYSYAGPDRLDITRQGADPRGIVLAPSWDILKPALAAR
                     QAGVDSWDRYRLDYTAEMRASYARTGWTELYACAELTFVCFCQDPARCHRTIAADLFV
                     RAGRGRVQYLGERIAPAPAQQSLFP"
     gene            complement(49316..49987)
                     /locus_tag="PKV97_00365"
     CDS             complement(49316..49987)
                     /locus_tag="PKV97_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04355.1"
                     /translation="MQDQNKIYRAYLSAVAYREKPTAAPPETDGSLEETLAIAIALTQ
                     SCGMLCTKQNLLVRVDGFLNQPAAQNPPGVGAPKEAAAGAPPSIAEEEHADDQFHRWQ
                     EDGYTAIKDDKPVDTFLTPMALWESSFPDRRSVLFVKNSSDEIFGFDVFTGKCIDDDG
                     LSLDAAGMERLAGARLGNIPDNYPGVTIFPPYNTMATKEAIMNKIASIPRNAAPTPTD
                     SSPSS"
     gene            complement(49990..50061)
                     /locus_tag="PKV97_00370"
     tRNA            complement(49990..50061)
                     /locus_tag="PKV97_00370"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50027..50029),aa:Met,seq:cat)
     gene            complement(50067..50339)
                     /locus_tag="PKV97_00375"
     CDS             complement(50067..50339)
                     /locus_tag="PKV97_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04356.1"
                     /translation="MSSIVAGQTVSWTVRAQGVRRKAEGVVIAVCDSGESPKNRWPKG
                     LKQPENRELLGFDKPRQMAYAIVKETMPDGQIIYRTPRLQSGLVAA"
     gene            50439..50891
                     /locus_tag="PKV97_00380"
     CDS             50439..50891
                     /locus_tag="PKV97_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04357.1"
                     /translation="MTAVLARIEDLSFLEGKHRDEILDIISKEYSAPKEKVREWYNIA
                     VKRWRENQDGEDLTDRHYQLTRMAHSLYGRALNGVKIEQIHAIADELEKAITDPVKTR
                     ELITKLRECKAADIKTAQRLLWNMSAMAGLGNKVTVDLSGVNFQPTKK"
     gene            50912..52633
                     /locus_tag="PKV97_00385"
     CDS             50912..52633
                     /locus_tag="PKV97_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04358.1"
                     /translation="MQMHDRGMSAREIVNAIYKQTRLGISVADVQSVIDGMSRASSLN
                     KDLPPPPPLMEFIPQQQKRYRAPLHLKPFIDLFDQIGKKPMRIVGHVPPRHGKTETML
                     MAIAHGIRQDPSRLFGYSTYAMDLTLSKSIAARRYAEESGVRLVRDAAKEWRTKEDGG
                     LIATSVGGPLTGYGINAAMFVDDAYKNRVQAESKTVRNMIWDWLSDVVISRLEPGASL
                     FILMTRWHPDDLGGRALRELGKHKRTPEGARLCPADCPGCGPNPIDGFEEILLPALDP
                     YGNALWAERWSAEALLARKREVTTYTWMSLYQGTPRGRGSAVFSGVSFYDPGQLPKAG
                     FKLAIGIDLSYTESTSADWAVAVVLMQFGNAYFVLEVVREQLEAPKFAKILLELRKRW
                     KNPPMYSIFYGPEKGSIDFMRTLGLPIRGLKRAGDKFVRAQATAAVWNDSRIQVPQTS
                     RVEQHELVDGERICSEDCEGCGIGLVDYNHKLIDGQRTCDDDCKGCRFPWLDPFIEVV
                     TGFTGVKDDCDDDVDALVAAFEGIHESPAAMIEKIQALSRSESLRMPGDDIGPRRNDD
                     PFGAPSI"
     gene            52759..>53340
                     /locus_tag="PKV97_00390"
     CDS             52759..>53340
                     /locus_tag="PKV97_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04359.1"
                     /translation="MNTTENPAAVAEVQTTTAAEPGTPIEKKMIFIVRVNNSENKSQC
                     GFQYPEVGPVECPADCLGCRNDPCGCVWKPTEECGNGLHGWEHCIGDTTASSFINTEG
                     AKWQLIEVEDHADNLVRMDGKVKFRRGNVIITGKSHEVTAKLVELGGLRPGMLATAYT
                     AGYAGSATAGDAGSATAGNRGSATAGNRGGATAG"
BASE COUNT        10029 a        16424 c        17167 g         9720 t
ORIGIN      
        1 cagagcaggg gagtctctcg tcctttctcc ggtgcaccgg cagcatcttc cgattccgcc
       61 cccagtgcgg ccccccaggc agcgccgccg cctgcccccg ctcctgcacc ggcagcaact
      121 tccgattccg cccccagcgc ggccccgatg ctgactgaag ctgagttcgc ggagtacagc
      181 aagcgcctgt tcgcgtgcaa gaccgatggc gaggtcgatg cgctggtgac tgaaatcaag
      241 ggatccggcc gcaacatcag caaggacatg gcggggcggc tccggcaggc catcgagacg
      301 aagcgcaagt cgctggaagc ccaggcgaaa gccgcggggc agagctaaga ccatggccgc
      361 tacttcgtcg cagtggaagg tgggcgatgc cgcgatctac ggcaagcacg gactcggccg
      421 catcgaatcc gtcgagccga tgatcagctt tggcatcaaa aactgtcgca ttcagatctc
      481 gccgcagcag gcgcagatct cgctgcggcg tccctgcgac gaagcgcggg ccaaggacgt
      541 gctttcgatc attggtggcc ccgccccgaa gccgacggca gcatgctggg cgacgcgggt
      601 gcgggcgtat cgagacctca tcaatcagaa catccccgaa gaagtagcgg ggctttatcg
      661 cgagctcggg tgcaggcagc agctcactgc gagcgaaaaa cagattttcc aacaagcccg
      721 tgatcttctc gtcgctgatc tcactccgct gtatggagca aaggcgctcg acaagatcaa
      781 ggaagccgca ggcaaagcaa aatgaagccc atcaccatcg caaacgccga cgagtggatc
      841 gctgcgggtt ggcacggcgc aggcaatgcg ccacacgaca agaaagccga agcaggctgg
      901 cggcacgccg tgaatcaggc ccttgcgcac cggccgcaga tcatcattct cgggtccgag
      961 aaggccgcgg acgatgcggc gccgcgcctt catgcgcttg gctttgaagt ctggcgctgc
     1021 gagggcaagg gcatcaacgg cgtctatacc tatgtcggcg accagcctga aaatgccgag
     1081 gtgcgagtca tcggcgaggc ggcacagctc ggccaggtgc gggcgttggg atgcgaggcc
     1141 gcgggcgaag cgctcgaaat gaccgcgctg caagcactgc ccggcatcgg tccgaaaacc
     1201 gccgccgcga ttctcgagga cggctttcca gaagtggccg atgactggct gccgcggtgc
     1261 cgcggtgccg aaaaggtcaa ggccgtgctc gctggctatg cgcaggcgct tgaagaggcg
     1321 cggctcgtgc gcgtgcctat ccgcaagatg gaaggcccca aggacgatct acgcgcgctc
     1381 ggcctcggcc gtctcgcgga ccgcatcgag agcgagccgc acattgagca cgatggcgat
     1441 ccatgtccgg cagacgactt cggcgatggg ccgccggtgt tcgatgacga tcccgcgcag
     1501 tttggcggca gcgatttcta tggcgacccg ttccagggag ttggccatgg ggactgatac
     1561 gaaagtgcag tggtgtcacc acaccttcaa cccgtggctc ggctgcgaca agtgcagcga
     1621 gggctgtaag aactgctacg ccgaggtgag cacgccggtg cgtgtggcgc gctccaaggg
     1681 cctcgagctc tggggcgcgc aggcgcagcg gagaatcgcc agcgaagaga tgtggaagca
     1741 gccgttgaag tgggatcgcg atgctgagaa ggcaggcatt cggcaccggg tcttttgcgc
     1801 ctcgctggcc gatgtctttg aggactatcg cggacccgac gccgatgcgg tgcgcgcagc
     1861 tcgctttcgg ctgatcacgc ttatcgcaca gacgccgcac ctcaactggc tgctgctcac
     1921 caagcggccc gaaaacgttg cgaagctgtg gccagtgacg ctgccaggga tccagcacta
     1981 cgacaacatc tggatcggct gcacggtcga gaaccaggcg cgcgcgactc agcggttgcc
     2041 agagctcttg aagatcccgg ccgtgattcg tttcgtcagc tatgaacccg cgctcgaagc
     2101 cgtcgatttt tcgcgctgga tgtggccggt gcattcgagc tgggattcga aatacaaaac
     2161 gcccgaggac gcgctagcgg cgggatctgc cgtggggcgc caccggcaag cgctggtcca
     2221 tgccgatgct cgctttatcg actgggtcat cgtgggcggc gagtcgggac ccggcgcgcg
     2281 gcgctttgac atcggctggg cgcgcagcgt ggtgaagcag tgccaagaag gtggggttgc
     2341 ctgttttttc aaacaggctg gcagcaagcc gtttatcgac gacaagcccc tgtctcttcg
     2401 cgatcgaaag ggtggcgatt tggacgagtt gccgccagat ctgcgggtga gggagttccc
     2461 cgatgccgcg tgatcccgag aaagcaaaag cgtggaaacg acgctatttg caaaaacaga
     2521 agatcgaaaa gtacggcgtc gaagcagcag acaaggacat gcgaggccgt catgggcatc
     2581 atgcgaaagg tgagcgcaac ggtcgttgga actcgagcgg tcgactgctt acaagccatg
     2641 gatatgttgc agtccgtgtc gatcctgagc atccgcgtgc atggggacca tcacacggca
     2701 gtcagcgcta tgcatatgag catgaccttg tagcggagga gatgatcggt cgccacctac
     2761 aacccaacga gatcgttcat catcgcaacg gcgacaagac caacaaccgc ccagagaacc
     2821 tagcagtaga gactcgaagc gatcacgctc gcgagcatac tgcggtgcct ggtgcccgtg
     2881 atgagttggg tcgttttgct ccggtgaatc ctcgcgtgcg aaagatgtcg ccatgagccg
     2941 caacaaaaaa gacggctgcg gtggacatca ccagcggctg cgcgccggcc cggatatcaa
     3001 gcaggcagcg cgggaaggac gccgcgctgc ggaacggcag aagctggcgg agctccgcgc
     3061 tggtgctgaa cctgatgcag tcgtgatgcc gaagaccgac gaagtatcga acccgcggga
     3121 ctgggattga accatggaaa tcaagacact cgacgaggcg cagggaacca cccgagagat
     3181 gatcgcaggg tactgcgagc gcaaaggctg gacgcgccgc ataaccgcca ctgggacgcg
     3241 ccagatccca cccaatggaa gtcaaagctg gtccgaagaa gatccttggt tcctcgttgc
     3301 gaggatcgct gaagtcgaaa aacggaccga gcaggagatc ctgcgagaaa tcaatccgcg
     3361 ccttcgagag tttccgggcg cagcggctca ggcagctcat catcgcggga aatggatgtg
     3421 cgtagataca tcgagcggtt ctcctgtgat tgttatcggc cgcttcaatc gcctggacaa
     3481 cttccttcat tctgagcatc tcgcccgagg cagcttcgtt ggctatggca tcgaggcaag
     3541 ggatgcttgg cggcaaagag acgtgcttcg tttctggccg atcgatgact tcggtagcaa
     3601 ggtacgctgg cctgaacgca acggggcgat gctgtgagcc tcgagccgat caggatcgaa
     3661 gacgtgctgt attggcatct ccgaaacgcg gactgcttcg agcagctcga gctactcaaa
     3721 gacaagtgcg tcgaccacgt ctttgacgat ccgccgtact ctgagcatgt ccacaacaac
     3781 agccggcgtg gtggatcgaa gggcgctccc aacgcaagcc ccgcgaccca agacgggacc
     3841 ttgatccgtc cgaagcgcgg tgcgcggctc gctatctccg tcgaaaggcc tctcgagttc
     3901 gagccgctga ccgatgcaga taggaaggtg ctggcgcggc actacgcacg aatcacgcgg
     3961 cgctgggtcg gcgtgtgctg cgatatcgag ggccggcagg catggcaaga tgagctcgag
     4021 gccgcgggcc ttcagtacgt ccgcaccctg ttctggcaca aggtgggcag cacgccgcag
     4081 atgaccggcg ataggccgtc ggaagggatg gaagcgatcg tcatcgctca ccccaagggc
     4141 cgcaagcgct ggaacggcgg cgggaaagac aacttctatt ccttcccgat cgtgcggggt
     4201 acgcatccgg atcggatgca cccgacgcag aagccgatcg aactgatgcg gcagatcatc
     4261 gaggacttca ccgaccccgg cgacctcgtt ctcgatgcgc atgcagggag ctgcaccacg
     4321 ggcgtcgcct gcatcgagaa gaaccgccgc ttcatcgggt tcgagcagaa gaagttgttc
     4381 ttcgataagg gctatgctcg acttcagcgg gcttcgcggc aagagattct gttccgggtt
     4441 gctcccacgg ggaaacagga aaagctgctg ctggacgtgc caggggcctc agtatgaacc
     4501 ccgagcagta cgaacgcgaa catcagcagg tggcgcggtt ctacctgcgc gagctactca
     4561 gcagcgccac gcccgaagac ctcatcgcct tcggaggtga gcttgacgga tcgcgccgcg
     4621 acaccacccg tgaaaaggtc tcgtatctgg ctggcgcgca gcttggcctg gatgcgcgcc
     4681 ccggctcgcg ggcctgggtc cgccgcggcc tgcacctgcg cgttgccgag aaccgccgcg
     4741 accgcgcgct gtgcgccgag atcgtgaagc gcaggcacta cctcggccgc tggccagcgc
     4801 cgatcaaaac gctgatcctg tcctaccttg cagacctcgc tggcgcaggc cctggcaccg
     4861 cgggagctgc cgcgctggtg atggtggcct gcctccccgc ccagagccat gtcacccgcg
     4921 ccttgggcct tgcgctatac gaggtgctga cgctggttcg gacgtggcgc gccgatgacc
     4981 tcgggccgca gctcgcgccc aatctcacgc cggccaccct gcgctgcgtc gtgaagcggc
     5041 tgcgcgcgga ttgggtggcg aagaagtgca gggccggcgg tcttgaggcc gcgccgcggc
     5101 tgctgctcac ctttgccgac ccggtgcagg gacacgacgg cgctacctat acagcggccg
     5161 gggctaccgc ttgcggtccg gcggctggcg ggaagctcgc gttttgctgg gcactcgacg
     5221 acgagatcaa ggaacggctg cgggcctgga tggtggcgcg agaggagaaa cgatcgtgat
     5281 tgagttcgat acgatttgcc ccgtctgcgg tctcgatgcc gacgtgcaag atgaagtctc
     5341 agcgggcgaa gaagtcgact gcaaaaactg tggcatgccg ttgatggtcg aagagtggga
     5401 cgatggaaca atgtctttgg tcttgccgag cgacgatgaa gccgagattg aaatcaaggt
     5461 agccgatgaa cccaatcccc ctcacccata aaggccgcgt ctacgcctac gcctgcggtc
     5521 gctgtcatct cgttcacgcg ggcgcctgcg aaggccaccg ccacggcgta aagacgatcg
     5581 ccgctctcgc tgcgcatttt cgcgagcagg ccgaagcctg ctgccgctgc catgagtgca
     5641 aggcgccgct cgctggtgag cgctggggaa actgcccacc ctgcgagggg aagcggaaga
     5701 ccgagttcga agcgatcaag gcgaagcagc gcgccgagga agaagcgaaa aacgaatcgg
     5761 tgaagacagc gaccagcgcg gaagccgctg ccgcccttgc tcagcacatg agcgatatca
     5821 gtgaggtttg ctgggccgcc ggctggatgg aaggcaccga atattcgctg tgggagatgc
     5881 tgcaaggcgg cccgcgcgag tggggtatgt gcagagtgag ccatgccgac atcggccgcc
     5941 tgcgcgactt gcacgaagaa tctggcgggt ggtggatctg gggtgagcag gggcagcggt
     6001 ttgttactgc tgcggagtgg ctgaagatct atgaggggcg gcccgcatgc tgaccgagaa
     6061 acagaagtgg ctaaacgtcc taaaacacat cgctttcccg atagtgctga tcgggggtgg
     6121 cctgtgcttt gtcatttggc tggcgacata cacaccgccc gatctgcgag atccaccgtc
     6181 gaagccacgc atgccgccgg ccgtcaagcc gatcgagact gaccattttg gctattgcat
     6241 gcgccgggcc tttaaccagg ctcgcgcgag ctcctactgc gctggcatgc aggaggtctg
     6301 caaccgcttc gtcgtcgacc gcgctttcgg agaggggcac tattacgatc tcgaactgtg
     6361 cctcgagttc gcggccgaga actgcgctcc tccctatgtt ctcgaccgtc agcagttgct
     6421 caaagagtgt cccgagatca aacgatgaag cccgtcatgc agacccgcac cggcgtccgc
     6481 ggaaactgct tgtccgcgtg cttcgcctcg ctcctcggtg tttccatcga gaaagtagac
     6541 ttttcgtgcg gcgactatac cgacggcaac tggtacgacg ttgcctgcga aaagctgcga
     6601 ccgttcgggc tgacgctggt gctggtcgat cttcgacccg agatcacgat tccggcggag
     6661 ctctacgtca tcgccaacgg cccgagccca cgcggccacc gccacgctgt gatctaccgc
     6721 ggcgatgagc ttgcccatga tccgcatccc gacggcggcg gcatcttgag catcgagtac
     6781 ctgagttttc ttctacccct gcacctggag gttccatgcg tcccaagttg atctacatcg
     6841 ccggcccgta caccccgacc gaagaatcaa gcgtcgagca gaacatcgca gccgcgcgta
     6901 aagtcgccga acaagtcgcc tcgccgaaga tctttccgat cgtgccgcac aacaccggca
     6961 ccgcgatcga gcacatcggc gactaccaat tctggatcaa cggcacgatc gaactcatgc
     7021 accgctgtga cgcggtgctc gcgatgccga catgggagcg gtcgcgcggc gcgaccgccg
     7081 aggtgcgcga tgcggatcgc gtccatcttc ctgtcttcta caacatcccc gatctcatcg
     7141 gctgggtcag cggctacact aggccgaagc acgggcaaat cggccagctc gccgatcttc
     7201 ttgccggcct gccgcagagc atctcgctgc gcaacgggga tcggacgctc gaggtgcgcg
     7261 actggcgcaa ggaagccgac gacgggatcc tgtaccgctg tcactggccc gagcgcagcg
     7321 cgcggatccg cctgaccagc cagttctcga cgttcgaaga agtcgaagtc gcagagcatg
     7381 tcgagccagc gacgaagtca ccatttcccg ttggtggtgg cgcgcgcgag ccggcgacgc
     7441 tgagggcgat catccttcca gcctccgtcg ataaagtcga gttctcgatc ggctttgtgg
     7501 atccgccgaa atgaagacct tcgactaccg cggctcgagc gcggcactgc atgtcaacgg
     7561 atccaacgtc cgcatcgact ggaacggcca gaccttcacc ggcaccgtca aaaacaaccg
     7621 cgtgcagatc gtgccgccgc acccgatcgc ggtggaagcc tcggccgcgc ttggcccttc
     7681 gacaacggcc gtagtccgcg acgtgcgccc cgaggcgaag gtgcggcacg acgtgcgcaa
     7741 ggcgatcggc gcgctgccag gggtctatct catggataac cccgtcggac atgccgagta
     7801 ttacgacgac aagacgaaca cgatccgcca cgtcgattac gggctcgagg aaggcagccc
     7861 cgatctcatc gtggtgctgc gccgcctcgt aggctgccag atgatagggc tcgagctcaa
     7921 agcgcctggg aagtcggcgc gcgcgaacca gaccgcgtgt cacgagcggt ggcgccgggc
     7981 tggcatcttc gtctacacgg tgcaaagcgg cgaagaggcg ctgctcgcca tcgccgatgc
     8041 aaggcggcgg ctcagcgagg acgaacccgc aaggcccgcg acggcggtgc agacccttct
     8101 acccatcgag agcgcgctat gatcgttttg gacccacccc aggattaccc cggcaagttc
     8161 aagggctgct cgcacctcaa tccttcccgc cgctgaactc ttttttcagg gcattgatca
     8221 cgtcgggagt cggggtgatt ccgtgccgct ttttgaactc ggtgacagcc cactcgacag
     8281 cgaaccaggg accgatcggc cgcccgatct tctcgccgcg ggctttcttg gcgagctggc
     8341 gctgcaacac cgacggcatg acggcgcgca tgccccgatc gatgagggcg cgaagctggt
     8401 ttgcctgcac cgtgctgagg gagacctctt tgatctcggc aagctggttc gtcttgctca
     8461 ccttcggcgt ctcgggctcg aggtcgccac cgcagcggac gcaggcggct tcgcgcggct
     8521 ggaccgcgcc acaggcccgg caagtggtca gtgatggagc tcggccgctc gcgagcttga
     8581 tcgggtcgcc ttcgagcgaa tagacgcgcg gatgctcgat cgggccgaga tcccagaggg
     8641 atccgcatcc gtcgagcagc accatctttt ccttgccgcc gcgcgcgcct cggccgccca
     8701 tctgctggta gatgcccggc accgagatcc gccgccagac cccgacgcat tcgatctcgg
     8761 ggaggtcgag cgccttgatc ccgacgctgt gtgtgcagac cacgaggatc tcatagctgc
     8821 ggaagcgcgc gcggaagttc ttgcgcaggc tcgtcttgct cttcgctgtc acggcctcag
     8881 cgccgacgcc aagctgccgg aagcgttcgg ccgtttcctc ggcgtgcttc gcgctcttgc
     8941 agaagtagaa gctgcgcatg cccgcggcaa actgaaacca cgcctcgacg ggatcccagg
     9001 ccagcttatc ctgagccttc ggcggggcat gggtgacgat tctgcgcaca aggtagggtt
     9061 cggggttgtg gcaggtgttg atcggtacct cagcgccaca ggagcacagc ccgtgcgtca
     9121 tgccccagcc gagttgcggg ccttcaatga gcttctcgta gaagttgccg agcgcggtgc
     9181 cgtcaccgcg ctgcggggtc gcggtcttgc cgatgtggat cgcgttcgga taggcgtgta
     9241 ggaggtcgag cgtctgcggc ccggcgaaga tgtggcattc gtcgaggatg aacacgacgc
     9301 gcggctcatc ctgaaagggg cgcagcccgc gctcgatgag cgtttgcagc gagcagacgt
     9361 agacggaaag gcccgcttgc atctttttgc cagcctggat gatgccgacg gggatgccgt
     9421 gctcttggag cttcgccgcg gtgtcgtcga ccacgaaatc gagatcgacg ataaaaatca
     9481 ctttgtagtc gaggccgagg aaatactgaa tctggcgaat cgacgtgtgt gtctttccga
     9541 ggccagtagc catgcattcg acgaggcgcc ggaagccggc atcgagggcg gccagcgtgt
     9601 cgttaaacag ccgtgtctga tagggacgtt ccatgccgat agtctaaaag aattttcttg
     9661 tgctagtgca aaatagttgc taggtaaatc ccatgactga accccgcggc acccagtcca
     9721 tcgacatccc tgactacgcc ggccttctcg ataagtacga ggcgctacag cgcgaccgag
     9781 acgacattca caagaacctc gtgacagtgc gagtggcact ggcggatatg gaatccgagc
     9841 gccatgggct acgcgcacag gtaaagatgc gcgatgaaga gatcgcggga ttgaagcagg
     9901 acatgggcgc ggacctcgtt gctgcgctgc gccgcgaaag agcgcaactt gatacccaac
     9961 ttgccggcgc gcggaaagag cgggcgcagt ttttcgccga cgcctcacgc aatgcgaccg
    10021 agcgggaaga gcagcggacg cgcgccgaga aagcggaagc agagcttacg aagttcgtct
    10081 cgctggtgcg cgcgtggagt gcggcagatt ttcaatacgc ccgcgccgcg aattgtggtg
    10141 agagcgatcc acttcaagcc gcagtcgaag cctataacag cgcgcaagcc gcgctgcttg
    10201 ccgccgtcgg catcacggaa tacgacaaac tgagcgcggt gatcagcgcg ggtggctgcg
    10261 agcacagaaa ggagcttgat cgcttgtatg ctccgaagcc ctgcaccggc tgcaatctca
    10321 cgatgccgct cgaatctggc aaggtctgcg atgcctgcaa aaactcgctg ccgcacgcgc
    10381 tgtggctgct tttcaccgcg actccaatga aagagcggca gcttgccggc gaagcgatct
    10441 ggcacatcct gaccgcgcgc aaccgccaga acgacgatcg gacggagctg cgcagcgaga
    10501 tcgagcattt gcagcgagtc agcaaagcgg cccacgaccg agccgagaag gccgagggcg
    10561 ccgtcgatga catgggcaac cagatcgctt tggttcgcga ttggtggtac cgccgatctc
    10621 gagaatggaa gcgcagcgcc cgaggcttcc gaacgaaggc gaagatgttt cacagcgcgc
    10681 tgctgctcgc ccacaaagag cgggacgcag ttcgggctgc gctggatgcc ttcacgcggg
    10741 ccgatacgca gccgcagatc gcgggctttc tggttgccaa tggcgaagcg gtccgcttcg
    10801 aaaacggcaa tgtgcttccc gctgatccgc agacgcagat cgcgcacctg cgccagatca
    10861 atacggagct gaagcaggaa cttgctcgcc tcacgaagcc attccctggt agcgagttcc
    10921 gagagatcga ggcacggcac aaaaacatcg cggatagcgc agaccttagc gagtcagcac
    10981 atgccgcaca caaagatcgc gctcgcctcc tcgatgccct gcgaaacatc gacgccgcgc
    11041 tgaatgaagt gctcaaaatc aatcagagcc tcaagcgcga gctcgctgcg gagaaatccc
    11101 gccagcggaa caaggaagac acctggggct atcagatcct cgtgcgcagc cggcggctgg
    11161 aacgtgcgct tgatagctcc gagcggaaac gtgctgagct cgcgatgcgg ctggtcgaag
    11221 agcagaagaa gctctcgaaa gtcgaagaga ggatcgagcg cgagcgtgca tcgaggcgag
    11281 cggcggaagg gatcgctgct atcgcaaaga aggatctcga gaagacccag gccgagattg
    11341 cagcacagaa aaacgatctc gccgatctcg agctcgagcg gcagaaagct gtggccgaaa
    11401 gcgccagtcg attcgactgt caccgtggac caggaacgac ggaaccgggg tgcggcgggt
    11461 gtgtctcgtg tcttctgagg atcgaagagg gaatggcagc gaaggtcgtg cgcgcagaga
    11521 aggaacggga cgcagcgatc actcggcttc gcgaaaccga cctcttgcgc cgtgagaaca
    11581 tcgctgaagc gcaatcgctg cgcgcccgct tggccgagtt gactccgcag ccaccaaagg
    11641 agtgatcatg tccgagcagt cgaagaatgt tgagcactat cgcaattcca ccgatccgca
    11701 aacgcccgag ctccgcctct acgtcgagcg agatgagggt ctggttatgc tctacgaggc
    11761 cgacatgacc agccggagcc cgaccgcgca gattgtgaac ttgcacgaca agatcatcat
    11821 gctggctgcg gacgcccgtt ggctgcggga cacgttgacc tcgatggatc tcgaggatgg
    11881 gccggatgat gcagagccac cactgggaga accatcatga gcaagatcga aatttacggc
    11941 gagagcgacg acatcatcac gatcgatggc gactatgacg acgaggttga agccatcgac
    12001 ggtcgctatc tgctcttcgc ggatggcacc gtggtcagcg gggagttcac cgagcaggga
    12061 gtgtggcggt tcaaggtcga aaagcagggg caggctaccg cgctgcatga gccggccgag
    12121 gagttcgaag acctgggatc cgacatggtg actctcgagt atcccggcga tctctcgctg
    12181 ctatgggacg gctcggagat gccgccggat gcaccgctcg aggatctctt gcgagctgca
    12241 aagattcgct tcgatctcat cgaggcgtgc cacttcgaag acaaagacga gaacgcgctg
    12301 gctgtcattc tcaaggcgct gcaagacaaa tgagaagcat aaccagcttc ctgctcggct
    12361 ttgaatgcga tgggcagtgc ttcgcgtggc atgcctatca ccgcgcctgc tcggctgggg
    12421 tctgtttgtc gaaggcgcgg agcgagcggg cttctcggct tgctatcgaa gccgaggccc
    12481 agcgccgcgc agagattgag gcgacagggc aatgacattc ctcagcatca tcgcgcccga
    12541 gccgcccaaa gagatcgcag agctcctcga gatgctctat gcgctcggcc cctttcacga
    12601 tgatcttggg aaggaaggcc gcggtcagat cgtgctgtcg atcctcagct tcgcgcaggc
    12661 gctgcccgat acggtgcagc tcgaccccat aaagacgcgc gagctcccga tgacggaacg
    12721 ctggcaacgc aaaggcgtgc tgcaagagaa gtggcggcac gagtttgaga tcaaatgcaa
    12781 cgcgctcctc ggtgcggaat ctccggtgcg tgtgctgatc tcaccgtact gcacgaaggg
    12841 cacgatctcg atcgacctcg tgaatcagtg ttgacactag caactactaa aggctagctt
    12901 ctccgcatgg attctcctcg gccacctcac gcgctcttgt tcaaccgcat cgcgctcgta
    12961 ttcgatgcct tcggtatcgc gggcgcgaac cgctcttttg ggcagaccaa gctcgcggac
    13021 agcatgggca tctcgccgag catcttgcgg cggcgcgcgt ccgaggcacg gcacgggact
    13081 gcgcaccgag ggatgccact tgatcagatc cttcgcatcg ccgagatcgc ggtacaggag
    13141 ctcaagccgt tgcaaggcga ggacgcgcag gtggtggtgg cctggatgct tgggcttgac
    13201 cgcctcattc acttcgcgat tcagaacaag cttgtgaaga cgcggggcaa aaacgcgatg
    13261 acgctcggct tcgcgcctgt cgtgcagcgg ctcgagcgtt gggtccatga gatggatccg
    13321 cggatgaaag acatcccgct accggagaaa cagaagattc ggccgccggc cgctggctac
    13381 gtgccttcgc tcgatccgag tccgcgatcg attttccggc ctggcatgcg gcgcgcgcag
    13441 ctcgacaaaa agacgcttga tcagacgcgg gacaagctcc tgcgcaagac aaagagggcc
    13501 tcatgaagac tttgtttatg atcatcttgg tcgcgccggt ggtgctgttt acgccgctgc
    13561 aaggctgctt cctgccgccg ccgccgactc cgcttaacgt ggtgccggtt ggtaagcctg
    13621 catcgccggg gtgggcgtag tcatgtctct cggtatcatc ctcaaggctg actgggaacg
    13681 gtttggcgag aagttccagg aagccaaagc agagcgcatc ccccaactgc gcgatctcat
    13741 cgcgagcggc aaagaaaatc ttgttcgctt gcagaagcag ctcgatgagt tgccggggtg
    13801 tatcgagaca tgggaagcgg aactgcgcga tctcagcaga cccgtacaga cgccgccgct
    13861 gtctgagatc ttggctctgg tgctcggccg cagccaggtc gttccagccg aggtgccgag
    13921 accgatcggc gaagatgcgc cgcagaagat ttcagcgccg ggtggtgaac catgagccgc
    13981 aaggcaaaca agcgaaacat ccagacggtt ctcggccgca tctctacgca gtgcaaagag
    14041 gatcccacgc tcgccgcctt ctgggtcgag acgatcaaca acgcctgcga cagcgcaggg
    14101 gatgctttcg gtacggaagg acagaacgat ccccgcggcg accagcggga ttgaaatacc
    14161 tggatcggta gctcagtggt agagcgcggg gctgttaacc ccgatgtcgt gggttcgacc
    14221 cccacccggt tcgcttcgag gcgatagctc agttggtaga gcagcggtct ccaaaaccgc
    14281 gggtcgcagg ttcgagtcct gctcgcgctc gctgaggttg acaaatggga aacagcgata
    14341 agcagtctcc acaaaaaaat caaattagcg catgggatga tttggaccat gtctgggatc
    14401 cggtcacgtt tcgatgcgcg tgtggaacac caaaaatcgc cgtggccact ggcaaagcaa
    14461 agcgctgccc actcgcggga tccagtccac ccaaggtatc tgcatgagca ccgaacgaga
    14521 gatcagactg cgcatcacgc cggcatcggc tgcgtactgc ggaaccggca ggatcgatgg
    14581 aacttcctgc cggcatcttc gcaacacgga agatgatgcg tggaagtgcg gggcattttc
    14641 gcaactaatc ccgattggtc gcgggatgcc gccgcgtctc ccggagtgca tcgctgctga
    14701 gaagtccaag cagccgtgga ccgagcagag gccgacggag cctggttact actggctgtc
    14761 gtttcccgca gacaatagac ccacggatgc gccgcctgtc tttgtggtta gcatcgacag
    14821 cagcccgacg gggcgcatgt cggtgacgag ctgccagata aagccggtga tggcgaacgt
    14881 gaatctgctc gacagctatt tcgacgggac gaagtggatg ccgcgcacga cgcccgtcga
    14941 cccgttcaag gaatcgccat gagcacgaac tgcacccacg cgaaatacat ccgcggtgag
    15001 ttgcggctgc ccgtcgcaaa gctcgcggag tttcagcggc tcgagctgcc tgctaccaac
    15061 ttccttcaga ttccctcctc ttaccgggat gctggcgacg aggttgtcat cgtcaagcct
    15121 tggtggtgtg gcgactggtc gggaaacagc ttcgatacgc tgcgcgacac cattctgcct
    15181 gcgacccgtg gcagcgccga tctcgttttc atctgggaag gcggcgaagg cttcactggc
    15241 ttgcgcgtcg aggacggcaa agtcacagag cacgatgtag agtttccctc ggtgccccgt
    15301 gctcttcttc gcagcagtag cggcctacta cttccccggt gatcgctacg acacgggaca
    15361 ggtcgcctgt cccaaagtgc cggccgcgct ccgcgccgct cgcggtttcg cgcatcgcag
    15421 cgcgccgtgc gggtccaagg cactcatctg ctacaagctc cgatgcgcgg tgggggtggt
    15481 cgcagatcgc ggcccctatg gcatcctggg cgcgcagggc tggcgctggg gaacccgact
    15541 cggcccaaag gaacgctggg ccggtgagtt tgacctctgg cacgggcttg ccgcggagct
    15601 cgactttcgc ggcaaagagc ccgtgcagta tacgtggctg aaaaaggagt ccactcgatg
    15661 aacccgatcc ctcccgttga agacttcgaa cccattgatc cgccggctcc gaccgcaccc
    15721 cttacgccgc cggcaccgcc cgagctgcgc atcgtgccgc cgccggccga ggaagtgacc
    15781 gcggccgagg ggccgccgcc ggccgagtct ccgccagcgc ccgccgaagc ggcgccgtcc
    15841 agcgaccaca atccgctgct cgaggcgctc gcccaggggc ctctggtcat ggatgtcccc
    15901 cgcaccgcga gcgaagcctt ccactttctt tcggtgcgtg ccgccgagaa ggtcatcgag
    15961 gaggcgaagc gcgaaggcgg cgctgctgcc gcgcagatgt ggggcaacat cagcgagacc
    16021 tataagggcc tgtccgcatt ctccgcgcag cttccccagg ccaaggcgcg gcacctcaag
    16081 tggtcctatc tggtcatcct cgaagcgatt cccgatagcg cggtcgagct cgggcagctc
    16141 ctgcgctggc tggagaaggt cccggcctgc gccgatgaca aggcagtgcc agccgcgttc
    16201 ctctggttcc acctcggcca gtgctcattc atccacgggc agtcgctgcc gaggccgcag
    16261 agaaccgcgc agggctggac gtgcgagcac aacggcctca cccgcgtgct gagcaacgag
    16321 gaatcctacg gccttcagca gcagcggcat ttctggtcgc agcgctacta tgaccgttgc
    16381 atgcagaccg ccgggatcct tctgcgcaag ctgtctggga tcgacgtgaa gttcgatccg
    16441 aacaagaacc cgctcgaggt ggtctgcatg aagtgcaagg ccacggtgcg cgccggcagc
    16501 tcgcacgact gccccgctga gtccgcagtg cccaccgatg gcgtgccgcc gatgcgggcc
    16561 ttcacgcggc ccgaagcgcc cgccgagccg agtcccgccg cgttcgatga tgcgccggcc
    16621 gcggaagctg ctgctgaccc ggccgcgccc gcgaacccca ctgaaggcac gccggtagag
    16681 tccccgatcg aagctgcgcg caagcgcgtc gaggagctgc gccaagagcg cgacgaggcc
    16741 cgtggtcagg acagctacga ccacatcgac gagctgttcg agaatgcgca ggaagagctc
    16801 aacgagctgc tcgcggaagc ggaagacagc ggagagaccg cggcggcagc cccggctggt
    16861 gatggaactg cggcagcgac cgaggcgccg tcccccgtgg aagccatcga agccgccttc
    16921 gccaccgcca tcgttcccgc ggatcccgat gttcctacga tgatcggcaa caaagttcca
    16981 gcgccggccg ctgttccaag cggaacagac gaagagccga cggccttcgc caccgcgccc
    17041 cgcgaagact gatcccgcgc tgcctacgcc ccgtcaggat tgacggggaa cgcgcaacgt
    17101 ccccactgac acgtccccgt tcgaaaacac aacgaagccg tgcagtcgtc gtcagacctg
    17161 cacacggtgc agcgatccgt gccgccgtcg cggatcacgg gtcgagttgt ctggatggat
    17221 agcgtcgagg aggtggcgca gcgagagagt gccacgccac agagcagaga aagcgcaacg
    17281 agcgccgctg cgcggagagc gcgcacgcta taccacccgc actgcgcggc tataggcagg
    17341 agcgttgcgg aagtgcgcgt tgatcttcgt cttctgttcc tgcgccgcgg taacgaagcc
    17401 ggccagcgtc gtcaccgggt agtagccgcc ggtgagcacg ttcgtcgcat ccgcgacttg
    17461 gtgatagagc gtggcgtcgg cgcgatgcgc ctcatagtcc gtgtagatct cgttgatcag
    17521 ggtgaagagg ccagcggggt aactcaccgc agcgcccacg atcgtgttct ggttgtcggc
    17581 gccgaaggcc ccggtgtgga ccatgcccgc cgtcgcgaag tgggcattga tcttggtctt
    17641 catctggttg accagcgtga tgaaggtgtc aaggttcccc tgcaccgcgt tggccgccgt
    17701 gatcgcattg cctgcgtcgc tcgcaccgtg gacgccggtg gtgttcacga tgtgcaggtt
    17761 gtactgcgcc ttgcagttgt tcatatcgac ggcgagatcg gcctcgtcgt tgatcgtggg
    17821 cacggcgccg agcacgtcca ccgtgtcgat gtcggtgtga taggccgccg tcgtctgatc
    17881 cggcttcttg ttgttgatgt gattgacgta ggccgtgcgg atctcgaggt acagcgtctt
    17941 gatcgcctgg aacatcgtgt tgtacagcga cgtgccgtcc atgtcggccg tcgtgacgac
    18001 gttcgtggtg tcgctgttca agtgcgcgta gatgtgggta ttgatcccgg cgttcgaata
    18061 ggtgcggtgc gtgttgtaga gcgacttcgc cgcattcacg acggcgacgc ccgtcagaaa
    18121 gtcggtggca tcgggcacgc tgatatagag ctcggtcccg ctcggatcga tctccgtcga
    18181 atcccaatgg gattttccgg tcgaatccac cgaggcgtga ttgccgttga tcgcggcaac
    18241 gatgttggcg tggggcgcgc tggcagaagg aacctgatag gccatcggtg gactcctaga
    18301 aggtgaaacc ggcgagattc acgtcgatga agctcgtcac tgagttggtg aggatactgg
    18361 ctttcttgcc aggcagaagc gggattttga ccgtgtcttg cacccgcgca tcgaccaggc
    18421 ccgtgctcaa aacgagctcc cgccacatcg gaaagccctc tgggccaatc gcaacaaacg
    18481 gcctgagcgc tgcaatcact gcaacggtgc tgacgccgtt tacttgcaga atcgcggtgt
    18541 cggcgatggg cggcagccgc gtcgacaggt tgatggtgtc gcccgtggtc gtggccttag
    18601 acgactgcac ctgcggcggc tcgaggtaga ggaactcgtg ccggccgccg acgcctttca
    18661 tgaacttcgc ggtggcatcg gtctgcacga cgccaacgag agcgtggctc gtgctgccat
    18721 ccttgtagag ccgatacgga tccggcggtg acagcgagat cacgaacttc ggggcgcccg
    18781 cgatccagga caggtagatg tagtaggtgt tgttgccaat gagcgagccg ccgccgtcga
    18841 gatttgcgac ggtgatatct gtcgcagtcg cgatctgcat gtagcgcggc atcgtgtcga
    18901 agctgacgac gccaaagacc ggctcgaaaa cgattttcgt gccatcggtg cagcgatacc
    18961 gcagcggcga ttgcgactcg gcatgggccg cgagccgctt gagcaaaaac gtggtgcggt
    19021 cgagaacgac cttttccggg tcgctgaccg tgggcgccac cgcgggctgg ttcggatccg
    19081 gcagcgtcgc atcggccggg tacgtatcga ggccggcata cgtggtcatc gttcccacca
    19141 cccttcgagc gagataatgc cgattgcgct accgcctgcg tccatcttcg cctgaaactc
    19201 tcggttcgtt cgcagcggga gatcgatcgt caccatctgc tgcacatcgg cgcccttgcc
    19261 gatcacaatc tcttgaaagt caggcgcacc caccgccgag atcagcagct tgtctgtcgc
    19321 cgcggtgctc gtgttgatca tcttgatccg cagccgaacc tttcgcgcat aggttggaag
    19381 aaacgaggac aggttcgcag tgaccaacgt ggttcccgag gtactgagca ccgggaaggg
    19441 gttatcgatc gtgtagtagt cgccctcttt cgagtacggg accacgcgct tgaacgagtc
    19501 ggtgtgaaac gagccgacat atttcaaaaa cgagtcaact ttttggaaga ccagcttctc
    19561 gtcggggatg ttcttgctga tcttgcgctt gaacgtcgat gagccgttgt cccacccgag
    19621 atagcagtag tagaccgtcg aggcatcgaa cgaggcgccg cccgtgtcca catcgaggac
    19681 ggtgaagctg tctgcgttcc atgccacttg cgcatagcga tagggcagga acgcacggat
    19741 ctgctgaatc cacgtcgaca gcgggctgat gtcgatcttc gcgaagtcgg tggatcgcag
    19801 gtagctgcgg atgttgagcg gctgatcgtt ctgggtggcg aggccgagct tgatggagtc
    19861 gcgcagcgca cgaaacgcgc ggttcacgct ctcggcgtcc gctacgtcgt tgtcatcggg
    19921 aatcgtgatt tccgtgacat cggcttccgt gcctggatat tgcgttgcca tctaccgggt
    19981 tactcccatt tttcgcgccg ccacttgatc acgtagttca gcgtgtattt ggtgccgtcg
    20041 tcccaggtga agccgttcca aaccgcgcct gcgtacacca cgccggccgc ggtgagatgg
    20101 tacgcggttc cctgctgggt tactgcccaa aagttgtcag cgatggtgcc gtggacggta
    20161 tcggcggact cgccattgag gttcccgacg gccagcgccc agacgatgtt gttgtaggcg
    20221 aggacaatgc cgccctgtcc cgtcgcgacg gcacggccat cgggcagccc gtacacagaa
    20281 aacaggcccg cagcggttgg cgaggatacc gtcgaccacg cggttccgtt ccaatggatg
    20341 atggtgccgc cagcgccgac ggcccagaca tcgttcgggg cgcgccccca gatgccgttg
    20401 aggtcgcccg tgacacccgt cgtggtgctg gtccagctcg cgccgtccca gtgcagcgca
    20461 acgccgccga cgccgatcgc ccagatgtcg gtgcgcgaaa acgcgagcat gcgacgcaga
    20521 tccgtggcca cgcccgaagt gctcggcgtc cagccgaacc cgacatcgaa caggatcacg
    20581 ccgccctggc cacaggccca gcgctcgaga ccagcgtagg cgaagcccga gaggttggtc
    20641 aggatgccac tggtctccgc gacccaggca gttcctgtcc agcgcaggat ggtgccagcc
    20701 tgcccgacga tataggccgt ctcggtgtcg ggctttatga agacgccggc taggtttgcg
    20761 gtcgtgccgc tcaccgcctg cgtccacttc gtgccgtccc agcgcgtgat gtagccagcg
    20821 agcccacagg cccaggcatc gaacgtggtg cgcactgcaa cgccagtgag ctgcgcgggc
    20881 gcgagcttgt agcggcccga gtagctgaaa acgacgccgc ggcagctcca ttccgcacca
    20941 gtgaactttt tgatcgtggt gtagatgcga cgcagcgcca ccggatcgcc tgtcaccagc
    21001 gcatcccagc gcgcgggcgc gccccacttc ggcaccggat ccccccagcg ccgaaacgcg
    21061 aacggaggat cgtgaatcac gacgaagaac gagctcgcgt ggaagttgag atcgatccac
    21121 cactcgcgcg cgccccactc gccttcggtg gcgatgtcgc gcggcgccgt cagcttcgtc
    21181 ggatggaaca gcgggttgct atcgggctgt agcgacggct ggaactgcaa cgtgtccgtg
    21241 atgccgtcgc tcgaatgctt atagagccag accggcagca tgccgacggt ctgatatccc
    21301 agacgctgca cctcggcaat gatgccttcc tcggtgcctg ccttttccca tgtgtcccag
    21361 gcatcagcta cctgttgccg ccgctgcgcc gggatgaacc gctcgaccca gtccaggttg
    21421 taattttgca gcaggtacgg cagcgcgccg tcgggcgcgt agcgatagaa acgcgccacc
    21481 acggcttcct tcgccgctgc gatgagctga tcgatgggct tggtgacaac ggcctggaac
    21541 gcttcgcccg caccctcgaa gtagtgccag ctcagaaacc gcttgagata gtcaataaac
    21601 aggggcatcg ctacctcggc ttccaggtga tgaagtcgag cgacggctgg aagttcacca
    21661 cctgattcgc accgagaacg aagtcgaaca ggccgttcgg cttgtagttc ttcacgccgg
    21721 ccggcgtcat gacgcgctcg tagacctcgg ctgccacgat gcgatcgccg atctgcgcgg
    21781 ccttctggta gtcgaggagc tgcgcggcga tggcgctgcg cgctacggtc tcgtcgtagt
    21841 ctggcaagag ctcaacggtc ccgcgcacgg gctggttctg caccgtgcag gagtccgcga
    21901 agatcgtgcc gagcggacag cggtaaggcc gcaccgccgc cagcacctgc gccaccactg
    21961 cgggtgcaac cgcgccgcta tccccagcga gaaacagcgt cgcgcagagc gggcgggcgg
    22021 tgccctggaa gtagttggtg aagaccgtga ctttcttcac ctggggcacg aggtctgtga
    22081 ccaggctgat gtagccctct tcggggtact gttgcagctt cgacagccgc ccgaagcgac
    22141 ggaggcagcg ggccttgatc tcggcggggc tttctgcgtc ggttccgtag ttggtgatcc
    22201 agcttaggtc ggtgccctcg gggacgtttt cgacggtgac gccgggcagc gacgtgttga
    22261 gctggttgat ctgccgctcg agcacgttgt acttggcccc agggctctcg gctcgaatcg
    22321 ggatccaaac tggcgccgcc gaggagatcg agatcggagt cgtgtttgta gagaagaacc
    22381 gcagccgatc ctcggtgctg gcgattgagc gcccgccctg gatggagtac gggccgttgc
    22441 cagcggcgac ggtgtatttg agcttgcccg tcgtgtaggt cgcaacaaac ggctcgaccc
    22501 ggtagaagtt acgcgcgatc gtcgggatcc acgagtcggg ggcgagcagg ataaacccgc
    22561 cgttggtgat ggccgggatg ttcttgtagg cccgcgacag cgtaatcgac cagccttcga
    22621 gcagggtccg cgggaacgat ccatcgctcc aatcgtcaac gggaaagacc cgcgtgccgt
    22681 tggccgctgt gatccctttg aggtcagtga tcatctcgtc gaagatgacc gccgagtctt
    22741 tgccggcttg gagctcctca aaggacagcg acattcagca cctgtgcatt caccgtcgaa
    22801 aggtccatga ccagcgtgaa ggtttcgcca gtgaccaaga cgatgaggat tcggacgttg
    22861 agctgatctt ttttggtgtc gagtttgacc gtggggcgca ccgtcgagac gcgctcttcg
    22921 aagacgcaga ctcggatgat atcgcgctcg aggctgggaa agcccttcgg atcgagcttg
    22981 ttccccgccc gctcggcgag atcgatgctg ttgccgtggt aggcgggcag ggtctctttg
    23041 gtggtgactc gacggaagat gttgtaggca acgacggcgc cgccggtgat gatggggaat
    23101 cgtgggtcaa tccccgtcgc cccgagcagg atgtcgctgc cgtagttgtc cgccacgggg
    23161 ggactctacc acggcagggc ggatccgatg ggctaaggcg cttttacgac ggatacggtg
    23221 cgggtaaatg gcacgaacat gagggccggg gtcttcgctg cgaagaagcg ccacgtcgga
    23281 ccgatggagc tgagcggccc gctggtgcag cgaccgagcg gatctttcat gaagaacgtg
    23341 gtggccgtcg tcgtgatctc gaaatcggcc tggtacagct cccgatcgag ccagccgaag
    23401 acctcggcag tcgtcgcgct cgggttgtct ttgatcgaga tcatccgcgt cgagcaggtg
    23461 ctgtccgtgt aggcggggta gacgctgatc ggctggttgt cattcgtcgg gacgcagcga
    23521 tcgccgaacc gggaccactc gtagatctcg cagcggtgat tgagcgtcgt gtcgtagaag
    23581 tagggatcgc tgcgcgacag cgactcgctt ccgtcggtgc cggtgagctt gaccgcatcg
    23641 aagcggacct cgatgcgcga gcccgacttg acgacgggat ccataaggga tgcgaaggtg
    23701 agaggtcgct gagcgggcag ggttcgaaga gatcatcaag gccgatgcga agtcctcgcc
    23761 gcggctcatc tcctgcgcga gtgtgaccac cgtccaggcc tgatcgggat aggtgtgtcg
    23821 tactggatgc agcgctcgtc gtcgatgagg cgccgatgct tgccattatc gacaagcggc
    23881 ggagcagcaa acagcgcccg cacgtctttg acggcggcgc ggtggtcgct caggtgatcg
    23941 accaagctac gccgcaaggc gcgactcctt ccaggcatcg tgcgcgcgct cgaaggggtc
    24001 gacctcggag gggccagccg cagccagctc ggcttcaagc tcgtccgcca tccggtcttc
    24061 ttcttcccgc gacagcgcct cggggctcgt cgtgtggccg atcggagcga tgtcgatgct
    24121 gccgtcagca gcgagagaaa ccagccaggt ccgtccgagt gcgtcgcgcg tcttcatgtc
    24181 catatagcta gcaactaaaa tgcactagtg caagaaaaat ctttctggta ggttctaccc
    24241 atgtctctcc tcagcaaaaa actcgctcag cttctcgaca agctactggc gcgcatcgac
    24301 tacttagcca gctacgagtg caaaatcacg aaggtcaacg cggatggcac cgtcgatctg
    24361 gacccgttcg atccgcggct caagccgggg cctcagaacg tcgagctcga ggtgcctgcg
    24421 ggtttcgaga aggtgacgcc caagacgggc tctctctgca tgctcagctt caagaacggc
    24481 aagcaggacg cgcccttcgt cgggtttttc aagctcgggg ccaccttcag cgagatcgcg
    24541 ctgcgcgccg acaagatcaa gttgaacggc ggcggggccg cggtcgcgcg caaaggtgac
    24601 tcggtggggc agctcactgg caagaccgcc cccggcggcg gcgccgtcac cttcacttac
    24661 accagaactg gccagccacc cgtcgaatcc ccgacggtgg ctctcgccat tgacggcggt
    24721 aacgataccg tcgaagccta ggaggctgcc atgttgtcaa atctcgcgtt cctcgtcgcc
    24781 gagcacgcga cgcgggtgat gtccgaaccg ggatcgccga cggtcgagca gatcactgca
    24841 ctaaacaagg cggtgaacta cctcgagacc gccaacggca tcttccggca acttccctct
    24901 gattttgagg gcatggaggg tgcgggtgaa gccgcagtga cagcccgcgc cgtcatcagt
    24961 cagtggaccc cgtcggcccc cgttgcttcg ctgccagaat ctgcgcatcc agatcgatga
    25021 tccgcttctg tagctcgact tgaagctgtc gcagctcaag cagcagcagt cgcggatctc
    25081 ccgccgtcgc aagcgaacgc tgaactcgaa ggagttgcgc ggtgtaatcg tggcgcagcc
    25141 aggcggtgaa cacgcccagc atcgcctgca cgcgcgcggt cagcgggaga tcctgcggat
    25201 cgtgaggtat tcgacggcgt gctgctgctc aacgctcaaa acgcgcggcc gtgcctcccc
    25261 ttcctggata ctcgcgccgg ccgttttggc gatggccaaa atcgcgcaca gtccgacatc
    25321 gcccagagtg gcgatcagcg tatcggtcgc tggcttccag tcgctcgaac gcagcgcggg
    25381 cacgaccttg gggatcgcag tctgcaccgg cagcttcatg cagtcgatga acggcgcgca
    25441 gccggggagc agaagggaca gcacccagaa cttccatgct gcaaaacggg ccttcattgt
    25501 gcgacctctc cgacgggttt ggtcaggtat acatcgcagg gctgactcac ctgcacggtg
    25561 agcacgaacg aggtctggcc gcgccgggcc gttcccagcg ccacgatcga accagcggca
    25621 ccgctgactg tggaaaacgc gcccatgtcg tcggaaaacc ccaccgaaaa ccagccgttg
    25681 cactttgctt cgtggctgaa cgcgccgctc gcggtgaggg ccttgccggg catcgtcgtg
    25741 agcttccgca tcagcgtcgc cgggctgctg ttctgcaaat gaaaaacggc gacgcagggc
    25801 tgcaactcca ccgtcgatgg attcggcgcc gtgcctgccc agatatccgc cagcggcggg
    25861 tcacaggcgg cggcgccatc ggtgtcagca ctgccgctca gatctccggc atcggtcgcg
    25921 ccgaggtcga agaaccgcgg gtcgagcata taggtgcagc ccgagagcag aagcgctgcg
    25981 agcgcaccgg ccctcattgc acggtctgcg tgatcgagat cacgttctcg atggtgacat
    26041 cgcagttgag gtcggacagc gccgcgatca cgtacttggt ctgcggcttg cgcgcgatcg
    26101 cgtcggtgtc gaggatgccc gagcgcacct ggcccagcac cttgccgaag gtaccgtcgt
    26161 cgtcgatgaa actgagcgcg agcgtgccag tgctcaggca cttcgtggtg aactcggcat
    26221 gcagaatcag cttctcgccg accttggtct cggcctgctt gcccaagtag accggcatgg
    26281 tatcgcgcag cgaaaggaag gtcttgcaac cctcgaagcg caccgtgctc ggcggcttcg
    26341 catcgacgat ccagcccaac gtatcgagcg gcgagcaggt gttcggcggg accgcgagat
    26401 cagcgccggc cgagggcgga tccgtgctaa cgggaccaga ggcgcaagct gaggcggcgg
    26461 cggaaaaagc gaggagcagg gcaccgagga ggggtgagcg cataggggaa agatattccc
    26521 cgtcagcttc acaggtccag cgagatcgac ggtatcggcc ctgcgtagcg gggtatagcg
    26581 cgcgccaagg atgaggtagg aactggccgc gcaccgcact tgcactgccc gtttttgcac
    26641 gagcagtcgt cgcggcagtc gatgcacttg gctggctgca cgtcctgttg cggtctctgc
    26701 gccagcgtgt cctgcacgcg ccacgcgatg atctgcgcga tgaggcccac cccgaccgcg
    26761 atcacgatga ggacgttgat cggcggcagc gaatcggtga gctgcttgcg gagctcgggg
    26821 tttttctcag cggcctggat ggcttggcgc tgcttgtggc ggtcccaaat acgcgccagc
    26881 acggcgccga agacgaggat cgacgggagg ttgccaagaa gctgctgcaa gatctcgcgc
    26941 atggctgggg gctcctacgt ggtgaggagc atcatcagag cacggtcgcg gggtcttcgt
    27001 aaaagatttc cccacgccag atcccgcgtg cgaggtagta ccgcacctcg acgacgcggt
    27061 agctaccgta gtcggttacg agggtggttc gaggctgaac gatcgcgtcg tcatcggcga
    27121 agtcgagccg cttccattcg gggcggtcga gcggcagcac atccagggta tcgagagtga
    27181 cctcgggata atcgatctgc ccgatccgga cacggccatc gggctcgaac cagtaggcga
    27241 ggcccaggtg ccgcacgagc tgcgccagcg cgttgtctgc ggtggtggcg atgcgcatcc
    27301 agtgcgcgat cgcctggtcg cagatcgccg gcatctcagg gaacagggtc tcgccgctgc
    27361 cgtttaggat ctgctcgaag atggttcgcg gagtcgcttg gtagaagtgc tgcccctcga
    27421 cgggaaactt cgagagggtg ccagcgcctg aattgaagtt cgcatagacg cgggtatcgg
    27481 acagaccgcc cgatacgatg gtgacggcga tgtcgacgcc gggcagcccc agtgtgacag
    27541 cgccgctgat cggatcttcc ccgtcgatct cgaatgagcc gcggatcgcc ccagactcgg
    27601 gccagatgag ccgcccctcg ataagtgcgc ggtcgcctag agtgaattcg gcggccatgg
    27661 ctatggctcc ggcggcgggc cttcgtcgaa ggagttggga acgccggccg gtgttggaat
    27721 cgcggcctcg ccagaaacaa agtctttgtc aatggcgacc tcgggagtag tcacgagatc
    27781 ctttttcgcg tcttttttgg ttggcgggtt cacctcttcg aagtgaacgg tgacttctgc
    27841 aaatagactc tgcggatcga ttacaggcat atcaatttga taaataatag cctgcgtgat
    27901 ttcgaagtca gccaatagcg gatggtaaag ctgacgcgat ttgattcgat tcgcatcgag
    27961 cttcggattg atctgctgca agatagattg ccagtcctga tactgctctg gcgtccagaa
    28021 cataaaaacc atcgtccact ttgggctttt gctgccagtg gacgttttct tttgaccaga
    28081 gcggttcttt cgcttccttc tgtcaatgtc gaaaccgaca tggcccgaga ttcccttcag
    28141 cagaaaggga gcctcgtcgc tcggaaacat cacaccatcg agccgtagca cgttccacgg
    28201 cgtgagttca tctttgtcga tgacaccgcc gatctcacct gaccaaaaaa gcaggctcac
    28261 gatggagctc ccgccgccag ttcatcatcg agttcttcga ggaaccactc ggtgaagttt
    28321 ttcttcagcg cggtctcgtc gcactggatg accacagtct cgatatagac gttgccgccg
    28381 tcgccgcccc ggcccagccc taagaacttc tcgccaccgt ggaccacagc gagctgcggc
    28441 gagccgaacg gccctggcac gacgccgccc ttgtcgaagg acgggacgcc gagcgcgctg
    28501 agggcatcag cctggcccgc agtcagattt ggcggggccg gcgctgagga catgccgccg
    28561 tattccgaca tcgttggacc cgccgatgca gactcaatgg gcgatttccg agcatgcttc
    28621 ataaagcgct ctttgctgta gttgccctcg tcggaaccaa gaacgctttt cttgaaccag
    28681 ccctggacgc cgaggccgag atccgtacca agcgaccatg cggcctgccc ggcgcccacg
    28741 gcgaggccct tcaccgcgcc tggcagcgcc ttcgcaaagc cacccacgat gctcgggagc
    28801 atcgcagcga gcgcttcccc caccgcggtg ccgaacacct gccccgcctt ttccagcgcg
    28861 ccgccgaggc ccttgccgct ggtgtcgtga atcagctcgc cgaccagttc ccaggcccgc
    28921 tgaatcacct tcaccagctc ctgcgcagcg actgcggcgt cttcgaagaa gcgcgccata
    28981 tcggccttgg tgatgttgct gaggcccccg aagagcgtgt tcgtcaagtc ctcgacgatc
    29041 tgggccatgc gctggccctc tttcgtcccc aggttgaaga acgacaagat gtcggtgagg
    29101 aagcgcttga gttctttgat ccccttgaac tcgtcgatgt tgaggcgcat gaggaacgac
    29161 ggcaccgctt cctcgatgtt cgacatgacg ccggccagcg actcgatgcc tttctttttc
    29221 gagtaggcgc cgagctcggt ctgcttgagg gtgcgcagca cggccttttc gaccgcgacc
    29281 tcgaagacgc ccgagctcac tttgcgctgg cccacggcat cgcggaccgc cttgtcgatc
    29341 ttgtcggtat agggtccgcc ctctttgtag ccgacgctct tgccaagctg cttcgccatc
    29401 tcttcacgga tgaaccgctc ggagatgccg gccttggcaa tgccagagac accggccgtg
    29461 gatgcttcgc cacgcgcggc ggtgcgtgac atccagaact cgatctggcg cgcagccttg
    29521 tcgccgccga aggcttgcac atcggcccag gcagctcgag ccgcatcgag acggcgcccg
    29581 gtaaactgct gccccagcat gcgcttagtc agatcgatga cctgaaggtt tgatgcgggg
    29641 gttaattggg caatatcaat cgtcttattc aattgcgaag aagccttgcc ccggcttcca
    29701 agctggatct cgagcgcagt cataagcaga ccgcgctctt tcgctgcatc tacaagagtg
    29761 ccaacaacct ttttgccata ttcgaagctg ctaccaacga tgctcaccaa tccctgtttg
    29821 agatatccca acgcagagac gatcccctga ccaatcttct cgaagtcctt tttgatttct
    29881 tcggacttcc tctttgcctc tttgagctga tcgacataca tcttcgtgcc gatcttggcc
    29941 cacatatcgg caaacggctt catctccgcc accgactgct ttgaagcccg ctgcgcggcg
    30001 cgcgccgctg cttcttcctt ccgctgaatc gcgcgcgctt cctgcgcagc gacccgctgg
    30061 gcagccttgg tctcgcgctc ggtcgcgcgc tgagcagcgg catcctgctt gcggagctgc
    30121 tgatcccacc acacctcgta cttgcgccgc tccacgtggg aatccagcac agaggagcca
    30181 ccaccgatgc gcccaccacc cccagaacca ccgcggccgg cgccaggcgc ctggatgcgg
    30241 ttcacaacct tctcgagatg ctcgtagcgg gcgatgaggc cattgacctt cgccatcgcc
    30301 ttatccatgc cgccgccgcg cacacgggac tcgaatttgt cccatgccgc cgcgccctgc
    30361 tcgagcacgc gcacaacttt ggcgagaggt ccaaggaact tgtctcggag ctcgaggccg
    30421 aaaacgtgct gttcgtaggt gccagatgga gcgcccatcg ccaacaacta tacgcgcagt
    30481 gttactcgtc gggactcagg tttttatccc gagagcaccg gcgaccaatt ttttgatttg
    30541 aaggagattg ccttcgacgc gctgtcggtg acggatttcg aggatggcac cgaggaagga
    30601 atcgtctgtg gcttcgacgc cacgggctgc gaggagcaac cgcgcctgta atgcagtatc
    30661 ggcctcggca gcttctacaa cttttttgtc tgcgcgttgc ctcgcagcgc ttccatctgg
    30721 tcctgtacct gctcagcgat gacgccgaac aggttggcct tgagccagct tttcgcgcgg
    30781 acttctttca gatacttgat cgcgccggct ggccccggcg tcggctgtcc ctttacccag
    30841 aacacggtct cggccgcaaa ggtcgtaacg tagccctgga tgtcggcgtt ctcctttttg
    30901 gagatcatcc gcgtcagacg atcgctcgcc tcaacgctcg gcggcattgc gacgatcgtc
    30961 atttcgagcg cttcgatgac gaacaccgac ggaggatcga aacccttgtt cacgagatgg
    31021 gttcgacact cttcgatggc tgcctgcaca tcggcttcgt acttggaagt gtcggaagtc
    31081 ggtgactcgg tgggggtggg gtcgttcatg cgcagaatct agcaacttct ctgcacgctg
    31141 ggcagtaccc agttcggaaa attacatctg aacgtcgccg tcccatttga tgcgcgagaa
    31201 gatgagcggg atctcgacgg tggaaagcgc cgaggtgccg ccttcctgac cgccgggctg
    31261 catccccttg aatcgcaccg cgatgatggt gtcggtgagc gtgcccatgt tccactcggc
    31321 atagctcacc gtgatggtat gccgtaccgc cgagacacgg atcgcacctt gggcaatcgc
    31381 gtcgtcgtac aactgcttca tgtacgcggc ggctttgtcc ttgagcatgc gcagcgaccc
    31441 gccctgcggc atgtaggcgc cggggccatc gcctacgacg aacggcgtac cgccggcgcg
    31501 cgcgagctgg atgtcggtgt catcgccata cgaaatcgcg agccactcga tttcgtcacc
    31561 gtcgactttc acgacgagag agctggggtc gtaccgagga tcgtctcggt caatttggtt
    31621 gaaagccatg gctaccctgt caccgctacg aggttggggt tgaggaaccc gagggtgaag
    31681 gccaccttct cggcgtaggc gtaggagttc atgagcagcg tcaccggcac ccgcgacgtg
    31741 aacttcatgt tctcgttgcg atcgatcgca acgtcgatgt cgatgaggtc gcgcggcacg
    31801 aggtagttgc ggagctgcgc ccgcatcagg ctctcggtct ctttggcgaa ccggccattg
    31861 atcttgccgt tgcggttcac ctcgacttta tcgccgagga gattcgtgcc gatgatcatc
    31921 gcgagattcg cggtgcgcat cgcgacgcgc gtgaacatca gcttgcggta gtcgctgagg
    31981 ctctgcgcca tcgtggcagg gctgtaggtc gctggatcgc ccacgaagaa gccgcgcttg
    32041 ccgacgatcg ttgcgatggt gaggaagcgc gcctgcgcga gtcctggcac cttgcgctcg
    32101 tcgtggctga cgtaggtgcg gccgtcgggt ccagtgacga tgccaggcag cgcgccgagg
    32161 ctggtagcgc ccaggtggtc ggtgtaggtc ggggtgttcg tgacgtgcgc agcgaccgtc
    32221 gtggcgagcg ggcgccagta ggtgcggccc gacaccgcgc tcagcaggta gccgtagcca
    32281 gcgaccacga ccacctgccc ctgcggcgcg acgaagttca agtagtcggc cttgagcgaa
    32341 gtcatccagg ccgcttcggt ctcgccagtg ctctgatctc gggcctcgac gagcgcccag
    32401 gcgaagcgcc cgtccgcctt catgagattg agctcggtct gcaccgccgt cgcgatggtg
    32461 gccgagcacg gtcccacgag gtggaagaac tcccaatcgc tggtctcgtc gatgcgcagc
    32521 gcctcgagca cggtgacgac atcggccgag ccgaagacgg gcgccgtcac gttcagtgag
    32581 aacgcatcgc ccgtcttgat cgtcgcgccg gtgaaagtga aggtgaggcc cgagaccggg
    32641 ttcgttggga cgtagacacc gccgaccggg atggtgaact cgggctcgta gttgatgccg
    32701 ccgtcccaac tgatctgcgc ggtcgggctc gtcggacccg cgacgttgcc gcccgagttg
    32761 atacgcacaa ggatcgagcg gaagccgtcg accggcgtac cagtcaccgc gagcacggcg
    32821 tcagagccgg ccttgagcgt tgccgtcacc gtgctgatga cacctggggt ggtggtgccg
    32881 cagcgcatcg catagccggt gccggtgccg ttggcgagct ggaacgcatc ggcctcgaca
    32941 agcggtcccg ccgtgaaggt atcgagcagc gcctgcttgc tcgtgatcga agtgagctga
    33001 ttgagcgggc cggcgctcgc ggatccgatg gtgacgcagg cgcgcggcgc atcggagagg
    33061 agaccaaact ggccatcgag gaaggtaacg gttacgagca tggttatttc acctccccag
    33121 tcgtcgccgg gggcgggtca tcgggagggg tcggtacagg cgcgggcggc ggctttggca
    33181 gccggatcgt gctcggcggc gggatacgcg gctcgacgtg gccggggaca tgggccttgg
    33241 cgcgcgcgat gccggcgtcg tactcttcgc gggtcagccc cgtatccggc ttccacgagt
    33301 tgtggaacag ggccgtctcg tgccagacga gaaggagctg cttgccgcgg atgagctcct
    33361 cttcggcaac gtcctgattg tgccattcct gcgccgtctc tctggtcatg gctgcctctg
    33421 catcgtggga accaccgtct gattttcgtg ctgcgggatt gccggccgca tctgcgacat
    33481 cggcacccga aacgagctcg cccaagcgta gccggtttgg ttgttgcctg atacttcaat
    33541 ccagttttcg cgctcaaacg tgacgtttcc gccccagtgc tctcggagcg cccaccaata
    33601 gcaccagcgc aagatctcgc agccgcggac cgtgccccgc ttccagggtt catcgttgcc
    33661 ctgacacggc tgcccgatcg aggccccgta catcgtgcat tggatgaggt cgagcgcctt
    33721 tttgaacggg cgcgggttct ggagattcgg cagcggagtg cctcggtgct gtccgcccgc
    33781 ttggcgcgtg tcgccgagcg tccagacaag atagggccgc gacatatggg caaagttctc
    33841 gacttcgcca aggaagaact gcacgtcggt gccgtccgtt actggcgcag cgggaatcgg
    33901 cggcggctca tcggggccaa gcccttcgac aaaccccgga ttttgaagcg catatcgaat
    33961 cgtcttctcg agttcatcga ggacctcggt aagcgaattt atttgccggt tcgtatcgaa
    34021 ggtcacaggg ggaccatagc cgagtgaaaa aatatcttga agtagtgcat tttagttgct
    34081 agtaatcagg gcatgccgac cgaatcgatc gcctccctct gctaccccgg ccgtcctggc
    34141 actgctccgc gagacaagcc aacggcagcg atgcacgagt gctggctcga atcttccgag
    34201 cagcggaacc gcgaacgcgc agcgatgcag atgttcccgc tgtctgtgcg tctcgccgag
    34261 ctccgcggcg aaccgctcag ccgtggggaa cttgtcgcga tccacaccta ccccggcatg
    34321 ggcggtgtgc cgaaccgtga ggagatgtgg ccgtcgtgat cgagcgctgg ctgcgtctcg
    34381 acgatcgccg acttcgaaac acgacgaaga gcgattccac ctgcgaggcg cggaccttcg
    34441 gactcaatgg ccagccgtgg cctcgcatca cggttgtcgt ccgcatggat acatacggca
    34501 ccgtcggata tcgagtgagt tggagccgca agactggccc cggagagtgg tggtacgagc
    34561 acgacccgat gccgcgcgac atactggttg aacttcctga aatgatcatc gcgctgctcg
    34621 acaagacagc ggcgctctct gagacaaacg cacaggagta gaaaatgacc accgtcatgc
    34681 acaccactgc ggcgcccgcg ccgacccacg ataacagcga cgacggatat ccgcagctcc
    34741 tgctcggcct gcgcgaagcc ttttccgaga ccgtggcaag caggccgccg ctgttcggag
    34801 tgaacaccga ggggctgttc gatgtgttcc tgtcgcacct gccggaagct gcgcggcagc
    34861 actattcctg tcgcacctgc cggaagttcg tggagcgctt cggcggcatt gtaacggtcg
    34921 acgatcaggg tgttacgcgc tccgtctttt ggaacctgcc caacatctcg gtgagtgcgt
    34981 tcttcatcgg gccgctgttt cagatggcgc gtttcgtcga gactcgcgag atcgagggcg
    35041 tgtttctgag ccaggatccc gagtggggcc agaccacctc gggcaagaac gaggccggcg
    35101 aggactatca ccacttcgcg gtgacgccgc cgccgtcgct gctgttccgg catcccattg
    35161 acagcgccga tcaggcggct gcgaacgtgc gggaagagct cgggatgctc gagcgcggcc
    35221 tcgccgagtt ctcgcgcgag accgtgcgca ccaccgagac ctacctgtcc tcgggggatc
    35281 tgtaccgcag cgagaagtgc atcgagatcg cgcggtggct ggtgaagcag cacgagatct
    35341 gggggctgaa gaacgcgcgc ctgcggaaga acctcctctg ggtctcggcc gcgaccgcgc
    35401 cggctggctg gtgccacgtc aagcagagca tgatcggctc gctgctcgag gacgtgaagg
    35461 cgggcctcag cttcgatgtg ctcaagcgcc ggttcaacga gaagatggat ccgcttcagt
    35521 atcagcggcc ccaagccccg ccgtccgagc agaacatcgc gcaggccgag aaggtggtcg
    35581 cgcagctcgg cgcccagggt ggcctcgccc ggcggtatgc gcgcctcgat gatgtgaagg
    35641 ctttgtggaa gccgcgggct gccgctgcgc cggcgcagga tagcggcgtc ttcagccacc
    35701 tcaagaagcc caaggccgcg caactcgtag cgctcccgca gcgcacgttc acctgggtga
    35761 agttccgcga tcagatcctg ccgaacgcga cgaagctcga gatgctgacc ccgcggggag
    35821 ctgcccaatt ctgcgcgctg gtcacggccg tcaatcccga tgccccaccg ctgcttcagt
    35881 gggactatcc cgacgcgcgg aatccggtga gctggtactt catcggcaac ggatcgctgg
    35941 cgtcctcgtg gaacctgcgg ccgaacacct acgtcctggt gacggcgatc acgcgcaagc
    36001 cctcgatgtg gaacgaagat cggccggcga cgaaccacgg ggacggggtc atcttcctgc
    36061 ttgaaggggc caagcccacc catcaccagc gcggcggcgg catgttcgtc gagcagatgc
    36121 ggagcgagtt ccacggcgtc cgccgcacgc tcgaggcgca ctttctctcg acgccgatcg
    36181 cggatgcgga gtgcgccagc gcctgcggcc tctcgctcgc cgccgccagc cagaagaccg
    36241 agatcgtgat tcgctacgaa accccgacgg agaccggcac tatcctgatc gaccgctggg
    36301 actaatcaca agctgatgcg cggggggcgc atcgaggaga ccaccgacga tgaaggtcca
    36361 catctacacc atgcttcgca cttcaaccta ccgccgcatc tccgacgact ggccttgggg
    36421 cttcaccgcg ttcttcaagt agcgcgcgcc ctacctcggc gtcatcacca accgctcaaa
    36481 gatccgccgc gccagctcgt gaaaatcggg cgcccacctt cctagccctc gactcacaac
    36541 cggcaccatc aggcgcgcgg ggatcgagcc ccagacatag accgatcccc gttggtggaa
    36601 ctggtaggcg tagtggttga gctgtagcag cgacaggttc cgcagttgat acggcgagac
    36661 ccgcagcgcg tttttgaggc cgtctcgctg gtcgaagctg cggcctgtga ccgttggcgc
    36721 ccaccactgg ccgtaggggt ctgtctgctg ctcgaagccc ttttcggcca agcccttggc
    36781 gagaagagca gatccatcga gaaacttctc ggggagctgt tcgaggggct tcacgcgctc
    36841 gagcagccag cggaaggggt tgttattaga aatcatcggg cggggtcaca atgccgctgc
    36901 cccaggcata gcgggtgcgg ctctcgtatg tggttccgaa cggcccctca gagacacctt
    36961 cgagatcggg gccgaacacg acgcgcggcg ccagcacacc gggcagcggg aacgctgtga
    37021 tctgcgggtg catgagcttt ttgcccacct gctcgcccca ggcccgcgcc cgcgccgcat
    37081 tctttgcgaa gctgtcccga tcctctttcc agttctggcc cttgcggtcg tagagcgact
    37141 cggcagagat gcgcgcagcg ttgatcgtga cggagttgtc ccacgccacg atctgctgct
    37201 tctggtagcg cgctcggatc ggtgccaaga gctcatccga ccctgccacg caggcttcca
    37261 agatgtccgg cggcggttcc gtcgagaaac catagaagtc tcccgagacg tagttcgggc
    37321 tgctcgcgct ctgcgcttca aatagcagcc gcagacccgt cggctcaggt cgattgagca
    37381 cgatgggccg cgtggtggcg cagggcgctg caagctcttg cgcggtcgcg ccctcaagca
    37441 gatcgaacgt gtagctgccg cgcgtcgaga actgccagac atcgccgtcg ccgaagtagg
    37501 tgggaccgca gccagggtcg aaggtgagcg tgatgccgac gcaggggatc gtgtagctcg
    37561 aggccagcgg aatcggacca gccggcgccc cgccgttcac cgagtagtag aactgcgcct
    37621 cgccgaagcc gcccgtgcca gaaatcgcga ccgcgatccg atagggccgc gtcacctggc
    37681 ccgacggcgt gatggtgccg gtgccgcagc tcagaattgg gcgcgagacc acgtaggtgg
    37741 tcgctgcaaa ctgcgctggc cccaccgtcg agaagttgaa cacgtcaccg atcgccatcg
    37801 tgcccgtgaa taggagggtg aggccagtgc cggcgagctg gtactcgaac gaggccggaa
    37861 tcgcgatcgt ggcgctccat gtcgcgccga gatcgaagct gtagcggaag tagcccgtcg
    37921 acatgatctg cacacgcacc gtgtagttgt tcctcggtgt gcccgagacc ttgaggacgc
    37981 cggccgcgct cgtcgtcgag acgatgctgg tcgatggcac tgcatcgccc gagcgcgtgc
    38041 agcggagttc caccacgtag ccgtcatagg gcagatcggt gacgcgaatc gtcccctgcc
    38101 cggtgccggt atgcgtcaca tccgagatga ggccggggtc aaaatcgctc ggccgcagcc
    38161 gcatgcgatt gaggacaagg gaccagagct gagacggagt tacgagcagc agtcggcaca
    38221 tggccgagag tgtaacaaag gacggctccc gaaggagcca ggcgcagatc cgcgatgcgg
    38281 ttgccctccg acacgaggtc ggcgcgctgc gggcttaggc gggaccagga tcgactgcga
    38341 tgcggagata ggtcttaccg cccgaggacg tggtagcggt cgcatcggcg gtgcccacat
    38401 cgtccccggt gtcacccatg ccgcgcagct tcgccaaacc acccgtcgcg ccgaccaggc
    38461 gatcgccaaa gttcggcgtg atcgcaccac actggacatt cgcgaagtcg tccttgacga
    38521 actgcaccgg ctcgttgggc gcggctggcg tcaccgcgac tccaacgacg cccctagcgt
    38581 tatcgccacc gcgcttgacg ccgcggcaga ccatgaggat ctgcacctgc gctcccggcg
    38641 ccgccgaggt ctcgaccatc gagaggcggc tgaagtcgag catcgtcgtt ttggtgccgc
    38701 cgccggccga gtaggtgagg gtctcgcgct gccccgccgc gccgtacagc gtgatcgaga
    38761 aagccccgca gcccatcgcc gcgatgactt gtagcttgcc aagcgccgag acgggagaag
    38821 ctgcgatgcc gaccgtcggg ccggtgagct tcgccgcgcc attcgtcgtg aaaagggaga
    38881 cgttcgccgt tcccgagatg aggacgtgcc ccgcctcaag cgcgacgctg tcgacgttga
    38941 ccatgcggta gcggctcatt cgttcaccac ctgtttctct tcgtggcgca cccagtccac
    39001 accgacgatg aggttcggcg tgttggtgtt ggccacggcg aatccgaagc ggtcggggtt
    39061 gaagccgaga taggtctcgg tgccgagcag caggaagctg ccgccgagcg tgccgaccat
    39121 tgccttgtag gcgttgccga ccttgtggat cacgagcttg ctgatgtcgg gcgcgacgcc
    39181 ggggcaaacg tagaggctgc cgggaacccc ggcgaagacg ccgccgaaga cgccgccgat
    39241 cgtggcggcc atgctgaagc cccacttccc gctgccatca gtgccgcagt agatgccgac
    39301 ccagttgttc gggtcgaagg cacccgccga gctctggctc atgaagtaga tcgcgacatc
    39361 gccagtcccg agagggaagt tgttcacgtc gaagctgcac agcgcgctca tgtaccagtt
    39421 gagcggcgac gaggaggcgt actgtcggct gatcgtggcg aagccaggcc cgatgcctgc
    39481 cgcgccttgc atcaggatcc agctcggccg ctccccgacc gagtagaaca cctgattcgg
    39541 cggcgcgctg taggcgttgc cgaagcggtt gacgccacct tcgacccagg tctcggtgaa
    39601 gtcccagccc gagttgagtt cgctgccggc gaattcctgg ttctcggggt tcgcctgctc
    39661 cgaaggcccc ggatccgttg cggtccacgg cgtccaaaac gtgttgaaca gtgggatccc
    39721 gcccgagtag ccgttgatca cggccatggt gatctgatcg gatgtcaggt cggggcagta
    39781 caggcagagg taccgctgcg tcggtagctg gatcccctgg aagtggttct ttccggtgag
    39841 cggcggaccc ggcgcaacac cgcgctggtt cgcgaccagc ctctcgagcg gcagccagcc
    39901 ggcgtcgtca tcccacatca ggagatagag gttcacctcg ccgttgatct tggtcgtgag
    39961 gtcgacgccg acctcgacgt agcccgcggg gatgagccgg gccatgggcg cacccatggt
    40021 ttgcggatca tccgtcggaa acgggccgcc gaaaatggga tcgatgatca tggggaactc
    40081 cagccgcgca ggcggcgcga actaccaacc gatgaagttg tagttgagcg cgccgttggc
    40141 ggcggtgccg gggatgaagt ggtagtaggt cgtgttgatc gtcgcgagct ccgccacgcc
    40201 gttgaggaat tcctgccccg cggtgaagct gaagggcacg ccgatcgcag tcccgatgcc
    40261 gaccgatgcg gtgttcgccg tggccccgac cgcagtcttg cggatgcccg tgacggtgcg
    40321 gaagaccttg acgcccgcga cggtgctgcc cgccgccgag gcccaggttt cagagacggc
    40381 acggccgaac tggtcggtgc catcgacgat gacatcgcca ccatcccagc tcgcggcgaa
    40441 ggtgacgcgc agattgcggc cccagctctc gatggtgccg atcgggccgg ggaaggtgtt
    40501 cgctgcattc gcggcgacgg cggcatggat gccggtggtg tcagccgcca ccggagccgc
    40561 gtaggtcggc agcgcgccac gcacctgcgc gccggagctg aaggcgcccg actgcgggta
    40621 gagctcgttg agctgcgccg cggtcacatc ggcggtgcgg ctgttcgggg cgtagagcag
    40681 gtagtagccc ggcttctcga tgtcgaagac gtagcggctc tggccattga gcggcggctg
    40741 cgcacgacgc gccggctcga aggggaacgg ctggggcgca ttgatcggcg cgggctgggg
    40801 cggcgcgctg gcagcgaccg gaaaccaggc cgagacaccg tcccagcgca gcagccagat
    40861 gtcgacgaag ccgttgacgc tagaggacag cgtgatgagc gcctcgccct tgggcgaagc
    40921 aaggaacggc gcgatgccgc tgccgagcgc accgggggtg ctggtcgggt aggtggtgcc
    40981 gaactgaacg gtgacgctgc ccatgtttct tgctcctacg aaatcgaagg tccgttctta
    41041 ccgtacaggt acgccacatc gaacgtgccc gtgttggtaa gccagacgtg atagacggtg
    41101 gacggatcag cgacggtgag aaacttcccg acgttgctct ttccgagcat ggtatcggcg
    41161 attgagaccg cgctcgacgt ggtcagcggc caccatcggc cgttctcgta aaagatcggc
    41221 tgaagctcga tggtgccgct cgtgacggct ccttcgagga tcaaggtgtt cgcgtgcagc
    41281 ggcatcgcgc cgtagatgtt gccgccgatg ctttggctct caatgccgtt ctgccggaag
    41341 cccacaagtt gcggggcctg cgtcggatag agcgtcttta cctgcgccgt ggcagcggca
    41401 gcgccgaccg ccgacttcgc gactgcgctg accgtggcaa acggccagac cgtctcgaca
    41461 attgcgggaa ggcccgcggg tggcgtgacg gtctcgatgc gtgcctcgag cttcggagta
    41521 ccgtcgggat tgcgcggatt cttgggatcc accgcggtgc cggtgatcgt cacgatcccg
    41581 ccatcccagc ccgaggcaaa cagcaccgag aggggctgcg ggccgagcag tgggttgccg
    41641 gtcaaggtga acgcgctgcc ggcgtcgttg ccggcgagcg cgttggtcac agtgaccgcg
    41701 gacagcggga tgatcttgcc actcatgacg atgcaccagg cgcagcgcga gctgctacgg
    41761 gatcctcgac ttggcgatca tgcgccagtc cgcgatgagg caggcgttgt cctctcgcga
    41821 aatgaatttg aactgcctct tctcgtcgta gacctcggtg ccaggacccc ggaagatgtg
    41881 ctccatcggg atgtggaccg aatagtacag cggcgcgacg gggccgttca aaatgcagac
    41941 gtagtagacc ttgggattgg tcagcgtcgg cagcacgatg cccacgaagt cgtagatgtg
    42001 cgcccagttg gtggccccga actgatcgcg gtcgcggtcg agcagatacg acagatcgcc
    42061 gcgcagatcc ggcggcacga tgatgccgaa ctggatctcg ccgctgacga tcggcaggtt
    42121 gctctcgttg cggtagcccg acatcgcgtt cacgatcgtc acgaggcccg gcacatcgag
    42181 cttctcgttg aagagattca cgaagttggg gccgatcgcg gggttgaccg ggttctggat
    42241 gtggttgttc gcgaacgcgg gccggcggtc gaagctgagc tgcacctcgc cgttgttgag
    42301 cgcctcggcg acgcggaagt ccgccatcat cgccgcgtcg ttgcccttgg cgatgacgtt
    42361 ggcggtggtc ataccgacgc gatcgctggc catgtccgag cggttgatga tgagaccctg
    42421 cgggccgcgg gtgacgtgct cgaagggcac gtcgtagccc tgcggcgact gggcgccctg
    42481 cggctcacca tcgacaagct cttcgacggg gccgaggccc gacggcggca ccggcaggtg
    42541 gctcttcttg ctcgcgccgg tgaacttctg cgcgagcccc gactcgaggg agaccggcga
    42601 ggcgccctgg taggcgctca tgtagtcggt cttccacgac tcgaacatcg cctgcgggtc
    42661 ggtcgtgcca ggaaccgcgg ccggcgtgat gatgccgctc gcgcacatcg acagcgcctc
    42721 tttggccgca gcgctgcccc gctccgccat cacgcggagc tgcgcgatgg catcggccga
    42781 attgccacga ccacggcggc tcgcagcctc ggcgagcggg ctcttcttct gggaatagat
    42841 acgggttccg agtttcatgg cgcgtgatcc ttagttcggc tcgatgtaga cgcggacgcg
    42901 gcccgaggcc gagaagccct tgaagatgcc gagcttcgac ttgccggcgg acagacgcga
    42961 caccgaattc gcgtcgaggg tgtacacggg caggttctgg tcggtggcca ggatggcatc
    43021 ggcgcccgtc gagttgtcga cctcgacgta gtagccgccg tactcgccct ggacctggag
    43081 atcgccatcg gccgcgcccg tggtgtccag gggattcgca cagacgccga acggcagaag
    43141 gcccgtcgtc gcagtgcccc ggatcacgta gccggaggtg ttgatcatga ggatctcgcc
    43201 catcgcgaag gcgaccgaac ccttggccgg cgcccgcttg acgatggtgt tgcccgctcg
    43261 ctgatagttt cgcacggcca tggtgtcggc cctccttacg cgatgagccc gagcttgcgg
    43321 gcctttttct ctttgaggtt cttcaagcgc tcgtcgccgc cgccggtgga tgcccagccg
    43381 gccagatcct tcgggtcggg ctcgaccgac gcgatgatgg cgtcacgatc cttggcagcc
    43441 tgctccggac tcgcagaagt cgcggcagcc gttgtagcgg cagcagtgcc cgtggtcgcg
    43501 ggctgctcgt ggttcgtcgc gatcgccggg gtcttcttcg ccttgaagta ggcgtcgagc
    43561 gcatcggcgg tcttgatatt ggcgcgacac cacgcttcct cggaagccgc ggggaccttg
    43621 gccttgttgg cggtcatgac ctgctcatac gccaacgact cggccttctt ctcggcggca
    43681 tccttggcag cggtcgcgtc cgcagtcgcc ttctcggcgg cctcggcgcg ctcgcgcagc
    43741 gcgggattgt cctgctgcat cgcagcgagg gcggcggcag cgcggatggg atcctgctcg
    43801 ccggtgatgg tcgcgacggc agagagcacg gtcttggcag cggtgaggct cgccagcgca
    43861 gtcatggcgc ccttgccaaa gcgctcggtg ccagccgcag ccagcgcagc tttgtgcgcc
    43921 ttctgcacca acccaccgat cttcgacgcc ttacaggaag cgagagcggc ttcgaccttg
    43981 gtcttcgcct cgtctggcag cttcgcgatg acgctggcga gcgaggcatc gacctcggtg
    44041 tcatcgtcca tgtcggcgtc ggcatccgtc atgcactcgg ccatcgcgcc cgcgcagtgc
    44101 gtcacgagct cggcctgcga ggcgcagacg ctggacaggt tcatcgcgct cgcgagcatc
    44161 ggcacctcgc cattgaggtg aatcgcgcac atcatctcgt ccatggccat cgaagccatg
    44221 tagcgcagcg cgtagccgtg gcgggtcgcg acgaagttcg ggtctttgta agccatcgtg
    44281 gcagcctttt cctgggtggt cacttccggg gcagcagcga gtgcaggcac tggcggcggc
    44341 tcggaagatg aagtcgatgc ggcgacagcg gccagcggtt ccgcaggtgc tggcggcggg
    44401 ggatcctggg atgcgacagc agtctgcact gtgcgatcgg gagtgccgtg cgaggactcg
    44461 taatagcccg cgaaggcatc gccgggcact tggaccgagg cgctggtcgg cgcattcgaa
    44521 gccgccatca gctctttctg accgagcgta gcaggcagcg agacgagccc gaagttgatg
    44581 aagcgaatga tccgcttcga ggagcgctcg taaagccacc acggcgacgt gaaccgcttc
    44641 tcgcggttcg cgatgatctt ggtcgcgagt ggggtccact tgatattcgt ggcccacagc
    44701 gcgcccttga ccacctcgag atcgaacgag gccgagtcga gcttgtcctg gccggggagg
    44761 tagtctttgc tctggtcgtg ctcgtagtcc gcagcgcctt tgtcgggcag gccgccggtc
    44821 cactcgttcc agtcttccat gaccatgcga gcggcttcgt catcgaagac gaaggtgccc
    44881 ttggtggtct tgaagtcccc ttttgggaaa atgaggcgtt cccgaggcgg ggttgtcgtg
    44941 tcgccgagag aggcgaggca gtacgcgctc gcccagtcgg gatttgagac cttatatcgc
    45001 tctcggatgt cactcactaa tcccaaccgt aggaggttca ctatgcacgc acaagataga
    45061 tcttgacgta gcaactaaac cgggctagct ggtgctgtat gcaaggactc ctaaaacgac
    45121 tcgcgaccgc gcttcactcg cactggttcg ttctcgacca cagtgacgag aatttctact
    45181 actacgagtg tcgctgcggg cagcgctcct tcgcctattt gccaggcagc caaggcgatc
    45241 ctgatttcgc atgggtgcag ggtctcaagc gcgcggaata cgcgcagctc agtccgaacg
    45301 cgcggaagtc tcgcgcgcgg cgcgagatcc tggccgaagt gcagccgcat atcgcgcgcg
    45361 gcaacgcact gcaccggcgc gcgcagcggg ccgagtcccg aattcagggg atgctgcgca
    45421 agctgatgga gctgccgcag cccgcggatc gcgtctgggt ggtctgggac cgcggcgccg
    45481 agaaaacggg taacactaac gtgctcagca cgcgccgcgt gttttttaag gccagcgagg
    45541 caggagacta tgcccgcgcg atcggctggg aaggcgcggt tcccgatctt cttggcagcg
    45601 agagcgcctt catgggcggt gagcctctca tcaacgtgag ggtcatatgc ctgccggtaa
    45661 aataaagctc tacgtctggc agccagaagg ccacggcccg agcagctatt tcgtaatggc
    45721 agcttctctt gaggaagcga tcaaggccgt gcgcaaggtg gctggccctc cgtcgaaaaa
    45781 gaccgcatat caaaatgggg ctttctacga cggtgagaac atcctgccac cgaagattta
    45841 tctgcccggc gaagtggacc aatgacaacg actgacgcga aggcaccgat ctggttccgc
    45901 cggctcagtc gcaagctgct gcgccatgca gatctttaca aaccctcacg gccgcggatc
    45961 ttgcaagtcg atatcagcgc caccgaatta cgtatcaagg tctggcgcgg aatcatgatt
    46021 gagctcgagt ttttggagcc acacgagcac aacgtcacct atgaagtaac ggttccaatc
    46081 ttcgacgaga agacgcagag caaagccgtg aagatttacg aggcagtcaa aaagaagatg
    46141 aggcttaaat gaaaaagcct catcgccccg tctacatccg ctgcgcgctg catgtcctgc
    46201 gcgtacccga aatccgccag ctcctgcacg cggaacaaga gcatcagtac gtgctcgggc
    46261 tcgacgtaaa cctacggctc atcgtcgtgc atctggtctg cgtgggaagc gtagatcagg
    46321 cccagggcca cgcccgcgac atttttcggg agctgattcg gcacaactgc cacagcttcg
    46381 tgctggtcca taaccaccct tatggcgatg tggtttcgtc aaaaggcgac cgagctgtca
    46441 cggcctggct gcgccagtgc ggggagtggc ttggtattcc actacaggcg catgtcgttg
    46501 tggccaatgg caagcagccg cgcgaagttg gcatgccgag gaaatcataa tatggctgat
    46561 aatccagaaa taatcagtat taccgttgat gatttggcga agatgatgga gcaggccaca
    46621 tttcgcggaa tcacccaagc catgacccaa catcttccac ggaacacaga attgcttcct
    46681 cggcggcact ctgaaaatca taatcttcgt cggaagacaa aaaagcgaaa aattatcgct
    46741 cgaaaattaa ttgatgactt tgagccaata cagtcaacac agcaacaatc tacgatatct
    46801 ggtcttcaag agatatctat gcctaaatgg aagcgcgttc gttaaccgcc accacgtaga
    46861 tctgcacaag ttcgggcggg aacttcgaca gatcgggctg aaagctctta ttcgcatatc
    46921 catacccgtg atggatatcg ccagcgacca gcgggtaata ctgcgtcacg ccgatcttgt
    46981 gcgcttcctt catcgtcagg ggctcgagga tgctgcgaca gccgcccata tgcagcggcg
    47041 gaatgcgacc gtcgtaccac caagcctcac ccgcaggccg caccacgttc gccagcgcgt
    47101 cgcagaactg cgaggtgcgc tggtccagcg tggcgaggaa gcgccagtag ggaaagatgg
    47161 ccttcacctc gggcgtatcg agcacgcgga ccgtggcgcg caggaacgcc tggttcacgt
    47221 tttcggtgta gacctgcgcg gtgcggtgcc ccgtcttccc ccagccgctc agtagcttct
    47281 ctacgagctc gtctctgcgc tcctcgaacg gcctgtcatc atcgggaagg tcgagcgcat
    47341 cggccgccat cgcgagctgc gcataggcgg cgcgggtgcg atcgtctttg gtgacgcgca
    47401 ccttcttacg gctgcgcacg gaccttgtcc ttgacgcctg cgatgccctg ctgctccgct
    47461 tcgaggcggg agattgcgag ctgcttggcc agcttctcgg ggccgagctt cgtataggtc
    47521 gccatcaccc gatcgcggag ctcttccggg gttcgagccc ctgctacggc tttggcgaat
    47581 gcacgcaggc cggggatcgc ggcttcgggc ttcgaccgcc gcagcacgcg cccatgccgc
    47641 tcgacgatcg atgcggatgc agcgccgccg ccgcgccgcg ggatctggtg ctggcgcgcg
    47701 aactcgtcga catcgagatc ggggtagacc acgagcagct tctcgatggc ctcggccgcg
    47761 gacttcgctg cggcagcgtt cttcgagcgg atctcggcgt tgcgcactgc atcctcgggc
    47821 ggggtgcagt cccaataggg gaatggcacc tcttcgagat cctcgatacc tcggttgtag
    47881 acgtagtacg ggtagaggat ctgctcgcgc gagaccgtgg cgaggatatc gacatcgttc
    47941 tcgaggcggt cttgccgcgg gcgatcttgc accacggcac gggccttact gccatgctca
    48001 ccgccctgca cgatgtcgac agcgccgagg atgaggatgg tcagcgcgtc atcgacgtag
    48061 cgcaggagct tctcgaagac ttcccaggcg ttgctcttcg gctcgaggat ctcgagcttc
    48121 atcccttcga gcaggttgat ggtgctgccg gtggtcagct tggccaccgc atcgatcgtc
    48181 ttctgcacat cctcgatctc ggccgccatg ccagggacgg tgaggacgtt cgagccggtg
    48241 aacttcttct cgtacttgag ccagtccggc agcgccacct gccgaatcag caggagcagg
    48301 ccgatcgcgc gaatcaggcc gccgtaccag ggccgttcag cgatccacgt cttgaacagc
    48361 acccattttc cctttccggg tgcaaggtcg ccgacgagat cgatgacgcc ggcttcgcgc
    48421 gtgtagacgc gccagcacag ccggctcttg tcccagtagc agttgccggg gtgccagatc
    48481 tgaagcttgg accgccgccg ctccatgtcc cagcggttct gtccgatgca gaagccctcg
    48541 aggatggtcc agaacgcggc ctcgagttgc acgctgcgtg ggaacatata ggcccagtcg
    48601 cgatagagct ctggatcatc gactaccagg ctctggtcag cggcatcgag gaaggaaaac
    48661 ttcagccctc gaatcgagag caagcgcgtc tgaagcaggc cggcggtgtg atcgtcgcgg
    48721 aacagggtgt cgcacaggcg cgcagactgc tggaatagcg ccgcctcgtg cgtcatgttc
    48781 gccgaggtga tcgaccccca ggtccacaca tccggcggca gcagcggttc ccactgcgtc
    48841 acattgaagt cgagcccatg aatctcttgc tcgggattcc gacggcggaa cagctttttc
    48901 gcagatgaaa agaggcccat caggatggga acgtatcacg ggaagagcga ttgctgcgct
    48961 ggcgcaggcg cgatccgctc gcccaggtac tgcacacggc cccggccggc gcggacaaaa
    49021 agatcggcgg cgatggtgcg gtgacagcgc gcagggtctt ggcagaagca gacgaacgtg
    49081 agctcggcgc aggcgtagag ctccgtccag ccggtgcggg cgtagcttgc gcgcatctcg
    49141 gcggtgtagt cgaggcggta gcggtcccag gaatccacgc cggcttgccg agcagccagc
    49201 gcaggtttca ggatgtccca agacggcgcg agaacgatgc cccgtgggtc agcgccttgg
    49261 cgggtgatgt cgaggcgatc ggggccggcg tagctgtagc gggcggtgtg gaggatcacg
    49321 aggagggaga cgaatcggtc ggcgtgggcg ccgcattccg tggaatgctg gcgatcttgt
    49381 tcatgatcgc ttctttggtc gccatcgtgt tgtagggcgg aaagatggtc acgccaggat
    49441 agttgtcggg aatgttgccg agtcgggcac cagccaagcg ttccatgccc gctgcatcca
    49501 gcgacaggcc gtcgtcgtcg atgcactttc cggtgaaaac atcgaagccg aagatctcgt
    49561 ccgaggagtt ctttacgaac aagacggagc ggcgatccgg gaacgatgat tcccacagcg
    49621 ccatcggggt caggaaggta tcgacgggct tgtcatcttt gatggcggta tagccatcct
    49681 cttgccagcg atgaaactgg tcgtcggcgt gttcctcttc ggcgatcgac ggaggcgccc
    49741 cggccgctgc ttcctttggg gcacccacac ccggcggatt ctgtgccgct ggttgattga
    49801 gaaagccatc gacgcgcacg agcagattct gcttcgtgca gagcatgccg cagctctggg
    49861 tcaacgcgat cgcgatggcc aaggtctctt cgagcgaacc atccgtttcc ggcggggccg
    49921 cggtgggctt ctcgcgatag gcgacggcgg acaggtaagc tcgatagatt ttgttttggt
    49981 cttgcattgt agcggggagt ggactcgaac cactgacatc ctgggtatga accaggcgcg
    50041 ctgccgctgc gccaccccgc tgaaaatcaa gcggctacga ggccactctg caatctcggt
    50101 gtacggtaga tgatctgccc gtctggcatc gtctctttga cgatggcata ggccatctgg
    50161 cggggcttgt cgaagccgag cagctcgcgg ttctcgggct gcttcaggcc cttgggccac
    50221 cgattcttcg gactctcgcc gctatcgcag acggcgatga ccacgccctc tgcttttcga
    50281 cgcacgcctt gtgctcgaac agtccatgaa actgtctgtc cagcaacgat agaactcatg
    50341 agtatggtgt gatactaaat caatctacag ggcgcaacta ctttgtgcgc tagccggact
    50401 tcatggccag ggcttaccga atgcttaccg atgaccagat gaccgcggtg ctcgctcgaa
    50461 tcgaggatct ctcgttcctt gagggcaagc atcgagacga aattctcgac atcatctcga
    50521 aggaatacag cgcaccgaag gaaaaagtac gcgagtggta caacatcgcc gtcaagcgct
    50581 ggcgcgagaa ccaggacggc gaagacctca cggatcgtca ctaccaactc acccgcatgg
    50641 cgcacagcct ctatggtcgt gccttgaacg gggtcaagat cgagcagatt cacgcgatcg
    50701 cagacgagct cgagaaggcc atcaccgacc ccgtaaaaac gcgcgagctc atcacaaagc
    50761 tgcgcgagtg caaggccgca gatatcaaga cggcccagcg tctgctctgg aatatgtcgg
    50821 cgatggcggg ccttgggaac aaagtcacgg tcgacctcag cggcgtgaac ttccagccga
    50881 cgaaaaaatg agattcccga cggaagcagt gttgcagatg cacgaccgcg ggatgagcgc
    50941 ccgcgagatc gtgaatgcga tctataagca gacccgactc gggatctcgg tcgccgatgt
    51001 gcagtccgtg atcgacggga tgagccgggc gagcagtctc aataaagacc tgccgccgcc
    51061 gccgccgctc atggagttca ttccgcagca gcagaagcgg taccgcgcgc cgctccactt
    51121 aaagccgttc atcgacctct ttgatcagat cggcaaaaag ccgatgcgca tcgtcggcca
    51181 cgtcccgccg cggcacggca agaccgagac gatgctgatg gcgatcgccc acggcatccg
    51241 tcaagaccca agcaggctct tcggctattc gacctatgcg atggatctca ccctctcgaa
    51301 gtcgatagcg gcgcggcgct acgcggaaga gtctggggtg cggcttgtcc gcgatgctgc
    51361 gaaggagtgg cgcaccaaag aagacggcgg tcttatcgct acttcggtcg gcggcccgct
    51421 caccggctac ggcatcaacg cggcgatgtt cgtcgatgac gcctacaaga accgcgtgca
    51481 ggccgagagc aagaccgtcc gcaacatgat ctgggactgg ctctcggacg ttgttatctc
    51541 gcgtcttgag cccggcgctt cgctcttcat cctgatgacc cgttggcatc ccgatgacct
    51601 cggtgggcgg gcgctgcggg agcttggcaa gcacaagcgg acccccgaag gcgcgcggct
    51661 ctgccccgcc gattgcccag gctgcgggcc gaatccgatc gacgggttcg aagagatcct
    51721 gctgccggcg ctcgacccat acggcaacgc tctctgggcc gagcggtggt ctgctgaggc
    51781 gctactcgcg cgcaagcgcg aagtcaccac ctatacctgg atgtcgctct accagggcac
    51841 gccccgcggg cgcggctcgg cggtgttctc tggggtctct ttctacgacc ccggccagct
    51901 cccgaaggcc ggcttcaagc tcgccatcgg catcgacctc tcgtataccg agagcacctc
    51961 ggcagactgg gcggtcgctg tcgtactcat gcagttcggc aatgcctact tcgtgctcga
    52021 ggtggtgcgc gagcagctcg aggcgccgaa gttcgcgaag atcctgctcg agctgcgcaa
    52081 gcgctggaag aacccgccca tgtactcgat tttctatggg ccggaaaagg gcagcatcga
    52141 cttcatgcgc acgctcggcc tgccgatccg cggcctcaag cgcgctggtg acaagttcgt
    52201 gcgcgcgcag gcgacagcgg cggtatggaa cgacagccgc atccaggtgc cccagacatc
    52261 gcgggttgag cagcacgaac tcgtagacgg cgagcgaatc tgctcggaag actgcgaagg
    52321 ctgcggcatc ggcctcgtcg actacaacca caaactgatc gatggtcagc gcacctgtga
    52381 cgacgactgc aagggctgcc gcttcccctg gctggacccc tttattgagg tcgtgacggg
    52441 cttcaccggc gtcaaggacg actgcgacga cgatgtagac gcgctggtcg ctgccttcga
    52501 agggatccat gagtcgccgg ccgcgatgat cgaaaaaatc caggctctct cacgctccga
    52561 atcactgcgc atgcccggcg atgacatcgg cccccgtcgc aacgatgacc cgtttggtgc
    52621 gccgtcgatt taagttgaca cgtagaccgt tttagttgct agctatggat atgcagcacg
    52681 cacccaccga ccccaggcga atgggcctgc gctcacgggt cgtgctgcaa ccctttccct
    52741 tccatgcaga ggagccccat gaacaccact gagaaccccg ccgccgttgc cgaagtccag
    52801 accacgaccg ctgccgagcc gggtacgccg atcgagaaga aaatgatctt catcgttcgg
    52861 gtcaacaact ccgagaacaa gtcgcagtgc gggtttcaat atcccgaggt cggcccggtg
    52921 gagtgccccg ccgattgcct cggctgccgc aatgatccct gtggctgcgt ctggaagccg
    52981 accgaggaat gtggaaacgg tctccatggg tgggagcact gcatcggcga caccaccgct
    53041 tcgagcttca tcaacaccga gggagcgaag tggcagctta tcgaggttga agaccatgct
    53101 gacaaccttg ttcgcatgga tggcaaggtg aagttccgtc gaggcaacgt catcatcacc
    53161 ggcaagagcc atgaggtcac tgccaagctg gtcgagctcg gcggtctgcg ccctggcatg
    53221 ctcgcaacgg cgtacaccgc gggctacgcc ggcagcgcca ccgcgggcga cgccggcagc
    53281 gccaccgcgg gaaaccgagg cagcgccacc gcgggaaacc gaggcggcgc caccgcgggc
//
LOCUS       DAOQNN010000002        48141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_166_length_48141_cov_9.997213, whole genome shotgun sequence.
ACCESSION   DAOQNN010000002 DAOQNN010000000
VERSION     DAOQNN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 48141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48141
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166_length_48141_cov_9.997213"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /gene="argA"
                     /locus_tag="PKV97_00395"
     CDS             <1..1254
                     /gene="argA"
                     /locus_tag="PKV97_00395"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNB04360.1"
                     /translation="SACAASAGEVIAGPAAQALAHDINLLAALGVKLVLVHGARPQID
                     EEMARRGLTPRYHHGLRVTDAEALECVKSAMGVTRLEIEALLSQGLPNTPMAGAYVRV
                     TGGNFITAKPVGVVDGVDYQYTGAVRRVIADEIRADLEQENVVLISPMGFSPSGEIFN
                     LGMEEVAAQVAMAIKAEKLVFLCDVNGITDGRGRLIPAMTADEVEKLLGGRRKLSEDM
                     GLYLPWAVKAVRGGVNRVHLVSEDVDGALLLEFFTREGVGSVITRDALARVRQATVED
                     VGAIIGVIAPLEADGTLVPRGRERLEMEIERFSVLEVDDVIIGCAALYPFNNDKAGEL
                     ACLAVMPGYRREGYGEALMHHIELRARKSRLQDLFVLTTRTAHWFVERGFREAGIEAL
                     PRRRRELYNLQRRSKVLIKSLAETD"
     gene            1270..3384
                     /locus_tag="PKV97_00400"
     CDS             1270..3384
                     /locus_tag="PKV97_00400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB04361.1"
                     /translation="MRRGPTHPFEQIRRAGRAPLRLWRTLGFRFAVVVMLVVGITVTL
                     LTVNGIRLLDASLIRRENQRLEEIRSLINATIALHLSRNELATVRHEIQAIRSDSGLQ
                     YVVLLDRDQRILTAEGWPVDRPLPAPVEHVDSISPPAGRIDTYAAVTREGRVWGHVLF
                     GVSTENLQRVRRQMVWQNLAIGLIAALLAGGASWLLGRLVTRELRALRAATAAVGSGE
                     TQTRLPVSRHDEIGELATAFNRMAGALNDRMEALVKSEARFHAIADFTYGVEAWFNPA
                     GRLVWINRSIERVTGFTPVECVLAPDLFDLLVYSKDRRAVQGAFALAMQGKPTPNFEV
                     RIQRKNGTTIWAGLNWQPIFGPDGEHLGLRVSGDDISSRKQAEIKLLDTVVELRREQS
                     LKEYYLKRSEEERFRLAALLDVLQLGILFVGRDRRVVYTNRAFRRVWGFAGTDDFDGM
                     RDTVLMERMDGHLIDAGAFRLHVDSVVKSREVTRPFEFTLRDGRTLSDASAVVSGPSP
                     IMYIGRIWIFEDVTERNRVAEQLTHLAERDPLTGLYNRRRFHEELDRMVADAMRRRTE
                     LGVLVLDLDGFKPINDNHGHHAGDQVLVRIARELGSVVRRSEMFFRLGGDEFAMLAQD
                     TSEFEMIGLARRVAAAIAELRFTFGGDRVSRITASLGIAIFPQHAENGEQLVTHADSA
                     MYQAKSAGKNHWRIYRGTSRAS"
     gene            3470..3742
                     /locus_tag="PKV97_00405"
     CDS             3470..3742
                     /locus_tag="PKV97_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNB04362.1"
                     /translation="MARMVKCVKLGREAEGLDFPTYPGELGKRIFENVSKEAWQQWIR
                     HQTMLVNENRLNLADARARKYLAEQMEKHFFGDGADGIQGYVPPGA"
     gene            complement(3806..4507)
                     /gene="phoU"
                     /locus_tag="PKV97_00410"
     CDS             complement(3806..4507)
                     /gene="phoU"
                     /locus_tag="PKV97_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNB04363.1"
                     /translation="MPEHTSKQFDAELENIRTKVLQMGGLVEQQVAKAMEGLASGEMF
                     VIDQVIANDNRVNLLEVELDEACSQIIAKRQPAASDLRTVMAVIKTITDLERIGDEAK
                     KIAKMAKRIHEAEAGFVPRIELRHVAGLAADMLHKALDAFARLDVQAAASVVREDSEV
                     DREFKSIMRQLITFMMEDPRTISRSLEILFIAKSIERIGDHSKNMSEYVVYLVKGRDV
                     RHTSLEEIEREASSG"
     gene            complement(4566..5231)
                     /gene="rpiA"
                     /locus_tag="PKV97_00415"
     CDS             complement(4566..5231)
                     /gene="rpiA"
                     /locus_tag="PKV97_00415"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNB04364.1"
                     /translation="MTQDELKQAVARAAIKYVPAGSIIGVGTGSTANFFIDELGAAEG
                     LIRGAVASSEATAARLRKHGIAVIDLNDVDALPVYVDGADEIDAGLSMIKGGGGALTR
                     EKIVAAVATTFVCIADASKRVALLGRFPLPIEVIPMARAYVERQVRAMGYEPRLRSGF
                     TTDNGNVILDVHGMQIANPAEVETRLNDIVGVVTNGLFARRGADVALIGGAEGVIELK
                     RPQ"
     gene            5369..5800
                     /locus_tag="PKV97_00420"
     CDS             5369..5800
                     /locus_tag="PKV97_00420"
                     /inference="COORDINATES: protein motif:HMM:NF039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HNB04365.1"
                     /translation="MGLLDGLAGQVLGSVLGGGAQGAQGGQGAAQLIQIVMGLIQNQQ
                     GGLGGLLQQFQQAGLGEQAASWVSTGQNLPIDAGQLSAALGSGTLGNIASQLGMSPND
                     ASGALASVLPQIIDQLTPNGQVTEQHNSLDGLAALAGQFFR"
     gene            complement(5871..7655)
                     /gene="lpdA"
                     /locus_tag="PKV97_00425"
     CDS             complement(5871..7655)
                     /gene="lpdA"
                     /locus_tag="PKV97_00425"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNB04366.1"
                     /translation="MSQTVEVKVPDIGDFKDVPVIEILVKPGDAVKAEDPLVTLESDK
                     ATMDVPSPAAGTVMEIRVQLGDKVSEGSLVLVLEAGTAEKPAAPASQPAPARAPASTP
                     ASAAPAAAGHYAGEVDVDTDMLVLGAGPGGYSAAFRSADLGLKTVLVERYATLGGVCL
                     NVGCIPSKALLHVAAVIDEAAHFAALGVEFGAPKVDIERLRAHKAKVVHKLTSGLAGM
                     AKARKVDIVRGYGSFLDPHHLEVELTTGDGQGKSGEKKIIRFRKCIIAAGSAAVRLPF
                     LPEDERIVDSTGALELRQVPGRMLVIGGGIIGLEMATVYSTLGAKIDVVEMLDGLMQG
                     PDRDLVKVWEKQNAHRFDQIMLETKTVAVEARPDGLWVKFEGRQAPAEAQRYDLILQA
                     AGRSPNGRKIGADKAGVTVTERGFIPVDAQMRTNVAHIHAIGDIVGNPMLAHKAVHEA
                     HVAAEAAAGQKSSFDAGVIPGVAYTSPEVAWVGLTEAEAKQQGRKVGVGKFPWAASGR
                     AIANGCDYGFTKLIFDEETHRIVGAGIVGPNAGDMIGEAALAIEMGCDPIDIGKTIHP
                     HPTLGESLGMAAEVFEGVCTDLPPIRKK"
     gene            complement(7652..8230)
                     /locus_tag="PKV97_00430"
     CDS             complement(7652..8230)
                     /locus_tag="PKV97_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04367.1"
                     /translation="MTTITVVKKNGQVAISAESLTTFGDTRLGAEYKAEHDKILHIGD
                     SWIGVCGSSAHHLVLAHLLAKIEDLRLGSRSEVFETFRKLHPMLKEQAFLNPKEDEDD
                     PYESSQITALIANPTGIYGVYSYREVFAYDRFWAVGSGRNFALGAMYAAYDKTKSAAE
                     IAGIGVAAGVEFDTASSGPIVTHAVKLIKEKK"
     gene            complement(8295..9908)
                     /gene="aceF"
                     /locus_tag="PKV97_00435"
     CDS             complement(8295..9908)
                     /gene="aceF"
                     /locus_tag="PKV97_00435"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HNB04368.1"
                     /translation="MSQIIEVKVPDIGDYKDVPVIEVMVKPGDVVKAEDALVTLESDK
                     ATMDVPAPAAGTVKELRLKVGDKVSEGTLVLVLEAGTAEKPAAPASQPAPAQAPASTP
                     APAAPAAAAATIEVKVPDIGDFKDVPVIEIMVKVGDAVKAEDPLVTLESDKATMDVPA
                     PAAGVVQALRVQLGDKVSEGSVILTLAAAGAAVATGAANAPAVAPAVSAQPIAAAPTA
                     PPPASPLATAPAGKAHASPSVRKFARELGVDVSKVAGSGPKGRILHDDVQRYVKGVMA
                     QVASGTAPTAAGTSLGGGLDLLPWPKVDFAKFGEIETKPLSRIKKISGQNLSRNWAMI
                     PAVTYHEDADITDLEAFRVQVNRENEKSGRKLTMLAFLIKACVNALQRFPEFNASIDG
                     ENLVYKKYFHIAFAADTPNGLVVPVVKNADRKGVFEIAAETGELAKKARDGKLAPTEM
                     QGACFTISSLGGIGGTYFAPIVNAPEVAILGVNKSAMKPVWDGTQFVPRLMLPLSLTA
                     DHRVIDGALATRFNVHVAQLLADMRRMML"
     gene            complement(9925..12597)
                     /gene="aceE"
                     /locus_tag="PKV97_00440"
     CDS             complement(9925..12597)
                     /gene="aceE"
                     /locus_tag="PKV97_00440"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNB04369.1"
                     /translation="MSALPNVLLQSDPDTAETQEWLDALEAVMAAEGPERAHYLLERM
                     IEDARLRGLNLPFSATTQYINTIPVEQQARIPGDQEIETTIRDYTRWNAMVMVLRANE
                     ETNVGGHISSYASAATLYDVGYNHFWHAPSDNHGGDLIFAQGHSVPGIYARAYMLGRL
                     TDEQLNNFRQEVDGKGISSYPHPWLMPDFWQFPTVSMGLGPLMAIYQARFMKYLHDRG
                     LAKTEGRKVWAFLGDGETDEVESLGAISVAGREKLDNLVFVINCNLQRLDGPVRGNGK
                     IIQELEGEFRGSGWNVIKLVWGTHWDALLARDKKGILMKRMMEAVDGEYQTYKAKDGA
                     FVREHFFNTPELKALVADWSDDEIWNLNRGGHDVHKIYAAYHAAIHHQGAPTVILAKT
                     IKGYGMGASGEAANIAHQAKKMSRDSLMKFRDRFKLPLTDQQVDDLVFLKFPEGSKEL
                     EYMRARRMELGGYLPARRRKADALAVPPLSAFEPLLKASGEGREVSTTMAFVRILNIL
                     LKDKQVGKRIVPIVPDESRTFGMEGMFRQIGIWNQQGQKYLPQDHDQLMFYKESKDGQ
                     ILQEGINEAGAMADWIAAATSYSTHGLQMIPVYIFYSMFGLQRTGDLCWAAGDMRARG
                     FLVGGTAGRTTLNGEGLQHEDGHSHLWSGAIPNCVSYDPTFSYELAVIVQDGLRRMYA
                     EQEDIYYYLTVMNENYEHPEMPAGAEADILKGMYSFRQGAASNGPRVQLLGSGTIFRE
                     VIAAADMLRKDWGVESDLWGCPSFTELAREGQANARWNLLHPTEKQKLSHVESCLKDT
                     RGPVIAATDYVKLFAEQIRAFVPRRYVVLGTDGFGRSDTREKLRHFFEVDRNWVTVAA
                     LKALADEGQLDRSKVAEAIAKYGLDVNKPNPMTV"
     gene            complement(12961..14283)
                     /locus_tag="PKV97_00445"
     CDS             complement(12961..14283)
                     /locus_tag="PKV97_00445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNB04370.1"
                     /translation="MFHRSPIQAPLLPRDEAWDWPRVVLGALILAGCLLRLLDPFYHN
                     PAFHLFSDPLRHWDNARMPLAPGPMALLDPPFFQVWLSIVQKWTLGHPALIAVYAATM
                     SLLTPWLWYRFLREMLSSRRLALLGWALFAWLPSWFDIFGYFMTETLFLPLLGASLWA
                     TARARRKQSTVSFCVMAVLWIFTALTRGIAAPIAVVAGFLVWYRHPLKWRAAAGSTLI
                     AVVILGPLAYRNQTTFGFWSPFGCGWLNEIYAASGKREINLKFRWGDGSWAYGFRSPS
                     LSYKQFEPLTDWTPVREGAVEVNVDLKQGARDWQAAAERTRASAEDLVRFRLENLALV
                     MAGETWPDSNQQHWTARLASSLRWIWAPLFVALLVVGAVTWRATLARPLVPAIIATWF
                     CVQAVSLLAINEGRYRKPLEGLLIVQTLIVMDTLRRRPDRLAPAWRCI"
     gene            14393..15358
                     /locus_tag="PKV97_00450"
     CDS             14393..15358
                     /locus_tag="PKV97_00450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB04371.1"
                     /translation="MISIICPLYNEEDAVGPFLERLIPVMESVGESFEILFVNDGSND
                     RTLECVLAARQADGRIRAVDLSRNFGKEAALTAGLDFARGDAVIPIDVDLQDPPTVIV
                     EFVRKWREGFKMVVGKRGDRRADSWPKRVSAGLFYRIHNRISDLQIPEDVGDFRLIDR
                     SVVDALRQFPERRRFMKGLFELVGFDTAVVTYARESRLAGTSKFSGWKLWNFALEGIT
                     SFSTAPLRIWTYIGLLMSLAAFLYAAFIVIKTLVLGIDVPGYASLLTVMLLIGGLQLA
                     GLGVIGEYIGRLYLESKRRPIYLVKGVFGEAKRPVTAGEAPLPGD"
     gene            15602..18232
                     /locus_tag="PKV97_00455"
     CDS             15602..18232
                     /locus_tag="PKV97_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB04372.1"
                     /translation="MAVGLFALAMFALVWGLQKREIDLERNALARDVQWAEQTMRLHL
                     QANQEFLVQLARDFAQGALTEPQFSAKTRQHVAGNPELTSVAWVDYDEKIIWAAPFDT
                     ADWMSGERLNANQALAFINAREAARPTYGEPYVDDRGKKLLDIYVPLFDGRQHVGAMV
                     GVYSIEGMLRHLVPTWFTEKYQLSMVIGEDKVLASSSATRPMEDAIAYVIPIEPIGHD
                     LKLRTAGYRTTSQLSRLVPAVLIVGLSLVIVGSLWALRMHLRRRVQVEKERDRLFNLS
                     LDILCIVGLDGTFRRINPAFERILGYPPDELAGTPLLELAHPEDMAATVEHLRALSAG
                     VPGTFENRVRAADGRYRWLAWSANPVPEEKLIYAVAHDVTQRRANEEALRAETSFRKA
                     MDESLVSGLRAIDMEGRIIYANAAFCRMTGFSQAELVGTAPPFPYWPPEDLDVCERNL
                     ALTLSGHIPSGGFETRVKRKNGERFHARFYVSPLIDSEGRQTGWMASVNDITEPRRAR
                     AALEASQERFIAVLDGLDAAVFVADANTDEVLFANRTFKAIYGFDVVRRSAEEVAPGL
                     SPQRNQYAVDPRALSADDLPRELFDGEVQNPLSGRWYHLRERATRWVDGRVVRMAIAT
                     DITDRKLIDEVNLQQQQRLQQTSRLITMGEMASSLAHELNQPLSAIANYNMGCVNRLQ
                     SGEYRVEDILAAMQKSSHQAERAGKIIRRVREFVRKSEPQRAPCRLAEIVEDAVGFAE
                     IDARKAGVSITIDVPRELPLVFVDRIMVEQVVLNLVKNGIEAMQGPVSDRRELDVEAH
                     VADDRHVELSVSDRGHGLPADAGNKLFVPFYTTKTQGMGMGLNICRSIVEYHDGRLWA
                     EDRPGGGSTFRLTLPIHLEE"
     gene            18235..18870
                     /locus_tag="PKV97_00460"
     CDS             18235..18870
                     /locus_tag="PKV97_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB04373.1"
                     /translation="MVDVQSGRAGGVQCVHLVDDDEALRDSLVWLTESQGFKVAAYDS
                     AEAFLAAWSPDMAGCLVLDVRMPGMSGLELYERLTQMHSTLPVIFITGHGDVPMAVSA
                     LKKGAVDFIEKPFNDKDMLRLIETCLALDREQREKRSHTAEIRRQLEQLTQREHEVLD
                     LIIAGKLNKQIADVLSISIKTVEVHRARVMEKMGARTLADLVQRVLSAGAG"
     gene            complement(18918..20792)
                     /locus_tag="PKV97_00465"
     CDS             complement(18918..20792)
                     /locus_tag="PKV97_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HNB04374.1"
                     /translation="MSQSSSNKNLSALVIGAIGVVYGDIGTSPLYAMKETFAGHHPIA
                     VVEANILGVLSLMFWTIMALVSLKYVAIIMRADNRGEGGSLALLALVSELTRANKRMR
                     WTVTMLGIFAAALFFGDSMITPAISVLSAVEGLNVVEPELSKFVLPITAGVLTALFVV
                     QKRGTGVVGMAFGPIMIAWFGVLALLGFQAIIRSPHVLAALNPAYALHFLVTDFHVAF
                     LALGTVVLAVTGGEALYTDMGHFGRFPIRLTWFGFVLPALVINYFGQGALLLENPQAI
                     ENPFYLLAPEWGQMPMVLLATAAAVIASQAVISGAFSVARQSVQMGFLPRMEIRQTSD
                     KAQGQIYVPFTNWTLYLAVMGLVIGFGNSSNLAAAYGIAVTGTMTIDTVLIGFVIILG
                     WRWPKALAIVAIGGFLIVDLAYFFANAIKIPQGGWFPLAMGLVSFAILTTWKRGRRLL
                     FNEMKRQSIPLEAVIEGLEHCPNRAFGTAVFMTGHDEGAPSALLHNVKHNQVMHERNV
                     LLTVVTEDRPYVSAEERVDVTDLGKGFHRVIVHYGFMQAPDVPAALELCAPHGLTFDM
                     MSTSFFVSRETVIPSLKRRMAPWRERLFCAMSRNAMSATDFFKIPTNRVVEMGTQVEI
                     "
     gene            complement(20899..21507)
                     /locus_tag="PKV97_00470"
     CDS             complement(20899..21507)
                     /locus_tag="PKV97_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HNB04375.1"
                     /translation="MNYLAHALLSGPDPESRLGGLLGDFVKGTPALFDPRLPPGVVAG
                     IALHRQIDRYADTHPAFQASRGRVSALRRRYAGIMIDMFYDHFLALQWPHYSDQTLHE
                     FAADTYALLADHAALLPPRLRGLLPHMREQNWLVSYADAAGIGAALDGISRHRLTRAN
                     PLAGGVEELLSHYAELEADFGTFMGAAVAFAHDWRDQTAAIR"
     gene            21660..22022
                     /locus_tag="PKV97_00475"
     CDS             21660..22022
                     /locus_tag="PKV97_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04376.1"
                     /translation="MRTSRKIVVGVALALAAGMVQADRGGRHGGHWHGPRVGVVIGAP
                     FGWYGPPFWYGPPYWYEPPVVVQSAPPVYIERGADVAGSEPEPYYWYHCGKPEGYYPY
                     IKDCPGGWKKVVPTPPSN"
     gene            22041..22529
                     /locus_tag="PKV97_00480"
     CDS             22041..22529
                     /locus_tag="PKV97_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HNB04377.1"
                     /translation="MKRLMIVAAAMVLAGCTTLPTGPSMLVLPGTGRNFDEFRADDAQ
                     CRQYAQDQVSGVTAERASVDAGVKSAVVGTVIGAAAGAAIGGHRGAGVGAGTGLLIGS
                     AAGAESASLSGYGAQRRYDNAFIQCMYAHGHRVPVSGNYSPERPRQPYYPPPPPPGYQ
                     QK"
     gene            22901..23494
                     /locus_tag="PKV97_00485"
     CDS             22901..23494
                     /locus_tag="PKV97_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNB04378.1"
                     /translation="MFQVRIHGRGGQGVVTGAEMLSIAAFLGGRHAQAFPSFGSERTG
                     APVVAFCRMDDKPIRLREPIMEPDALIIQDPTLLHQVDLFAGLKRDGYILINTNRSFD
                     ELGLGEFVKGFRHDRLLTVPATEIALKHVGRPVPNVPLLGAFAALGGLVTIDAVHAAI
                     REKFSGKLAEGNVAAADAACQFVREEEEEAARHAQAN"
     gene            23478..24731
                     /gene="porA"
                     /locus_tag="PKV97_00490"
     CDS             23478..24731
                     /gene="porA"
                     /locus_tag="PKV97_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HNB04379.1"
                     /translation="MLKQTEGSRAVAEAVALCRPEVICAYPISPQTHIVEGLGEMVKS
                     GALSRCEFINVESEFAAMSVAIGASAAGARAYTATASQGLLFMAEAVYNASGLGLPIV
                     MTVANRAIGAPINIWNDHSDSMSQRDCGWIQLFAETNQEALDLHIQAFRLGEELSLPV
                     MVCMDGFILTHAYERVDMPTQQQVDAYLPPYEPRQVLDPDAPVSIGAMVGPEAFMEVR
                     YLAHAKQMQALELIPQHAAEFRAIFGRDSGGLVRPYRCEDAETIVVALGSVLGTVKDT
                     VDELRDSGERIGVLGIGSFRPFPLAAVRNALEAAKRVVVLEKSLAVGIGGIVSTNVRM
                     ALSGLHLHGYTVVAGLGGRAITRASLHRMLRDAIADRLEPLTFLDLDWKLVNNVLARE
                     KQQRRSGPIAEGMLRDVGVVASRVG"
     gene            24744..25745
                     /locus_tag="PKV97_00495"
     CDS             24744..25745
                     /locus_tag="PKV97_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNB04380.1"
                     /translation="MSTQTIKFYQTGTFTVGNRLLDPAQRSVQATVARTNSINSGHRA
                     CQGCGEALGARYAIDAAMAATDNRLIAANATGCLEVFSTPYPETSWQIPWIHSLFGNA
                     AAVATGMAAALKVKRRGDVRVVAQGGDGGTTDIGFGCLSGMFERNDDVLYICYDNQAY
                     MNTGVQRSSATPPAARTATTMSVGAHPGNVFGQGKDVPAIAMAHGIPYVATATVADLR
                     DLEAKVEKAMSIHGARYIHILVPCPLGWGTASHETIRIARLARETGLFPVFEAAFGEV
                     TGASKIRHRLPVEDYLKPQRRFAHLFAPRLDVETIARIQQMADRNIRKFGLVEGEVQ"
     gene            25742..27379
                     /locus_tag="PKV97_00500"
     CDS             25742..27379
                     /locus_tag="PKV97_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNB04381.1"
                     /translation="MSRVVQTKPFAITLDVGSSLANHTGSWRTMRPIYLDRIPPCNHQ
                     CPAGEDIQGWLFHAESGDYEKAWRHLVQNNPFPAIMGRVCYHSCESACNRGRLDEAVG
                     INSVERFLGDEAIRRGWAFDSPAAASGKRVLVVGAGPSGLSAAYHLRRLGHAVTVREA
                     GPLPGGMMRFGIPKYRLPRDVLDAEVKRITDMGVVIEYNARVGDILASMREGFDAAFL
                     AVGAHIAKRAFIPAGDSAKVLDAVSVLRSLEGEEQPMLGRRVVVYGGGNTAIDMARTA
                     KRLGATEAIIVYRRTRERMPAHDMEVEEALQEGVMVKWLSTIRQAGESSLTIEKMRLD
                     EKGNPVPTGEFETLEADSLVLALGQDVDLSLIERVPGLEIRDGVVQVDRQMMTAYPGI
                     FAGGDMVPAERNVTVAIGHGKKAARNIDVWLRGATREAPPRHELADFEKLNTWYYSDA
                     PKTVRPVLDIIRRQSTFDEVLGGLDESNALYEARRCLSCGNCFECDNCYGVCPDNAII
                     KLGPGRRFRFNYDYCKGCGICVSECPCGAIKMVPEDI"
     gene            27874..28758
                     /gene="folD"
                     /locus_tag="PKV97_00505"
     CDS             27874..28758
                     /gene="folD"
                     /locus_tag="PKV97_00505"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNB04382.1"
                     /translation="MQARLIDGNALSATLRSHFAERAQKLAGRGITPGLAVILVGENP
                     ASAVYVRNKVAACEKAGIRSLRFDYSESVDPQEVFAQIEALNADPGVHGILVQLPLPR
                     QFDPEAVLDAVSPDKDVDGFHAHNVGRLAQGTPAFVPCTPYGVMKMLEAEAVPIKGAE
                     AVVLGRSNIVGKPMARLLLQAGATVTVCHSQTRDIAFHTRRADILVVAVGRPRMVSGQ
                     MIKPGAVVIDVGINRLPAEQGGKLCGDVEFESARAVAGLITPVPGGVGPMTITMLLAN
                     TLESAERHAARFDVWGAE"
     gene            28755..29261
                     /gene="purE"
                     /locus_tag="PKV97_00510"
     CDS             28755..29261
                     /gene="purE"
                     /locus_tag="PKV97_00510"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNB04383.1"
                     /translation="MNAMHVDPVVGIVMGSSSDWATMKAAALVFEEFGVPHEARVVSA
                     HRTPDLLFRYAETAANRGLKAIIAGAGGAAHLPGMLAAKTTVPVLGVPVQSKALSGLD
                     SLLSIVQMPKGVPVATFAIGEPGAANAALFAVAMLAREDEGLAAKLDDFRRRQTQTVL
                     DMKLSEHE"
     gene            29258..30385
                     /locus_tag="PKV97_00515"
     CDS             29258..30385
                     /locus_tag="PKV97_00515"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNB04384.1"
                     /translation="MILPPATLAMLGGGQLGRFFVIAAHEMGYRVAVLDPDPHSPAGR
                     IADEHLVAAYDDNDALERLGTTCAAVTTEFENVPADTLSFLSKYVPVRPSAECVAVAQ
                     NREREKSFFRDGGFPVGPFAVIGSEADIDAVPDDLLPGVLKIARFGYDGKGQARVATR
                     AEIKDAFHALKSERCVLERFLPLDLEVSVVLARTQDGEARCFPVAENRHKNGILDVSI
                     VPARVDDRLRAEAEELALGVALHLGYVGVLGVEMFVSEGRLLLNEIAPRPHNSGHYTI
                     DACVIDQFEQQVRALCGLRLGDARLHTPAVMVNLLGDVWHGNEPHWGRLFDDAGLKLH
                     LYGKHHARPGRKMGHFTVLDEDAGRAVDRAYAARERIGIRD"
     gene            30394..31383
                     /locus_tag="PKV97_00520"
     CDS             30394..31383
                     /locus_tag="PKV97_00520"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNB04385.1"
                     /translation="MHSDVVRAAELLRAGELVAFPTETVYGLGADALNPSAIAKIFAA
                     KGRPADHPLIVHLPPSARLDDWAREVPSGARRLAEAFWPGPLTLILQRQSQVPDQVTG
                     GQDTVALRVPRHPVALDFLEAFGSGIAAPSANRYGRISPTLARHVVEELGSAVSMVLD
                     GGACEVGIESTIVDLSRGEAVLLRPGLIGAERIESVLGRPLSVADALAKPAPRVSGSH
                     AAHYAPGTPLRLVRPDRLREVLNAQRHAGHRCAVIAYSQPAFAESPHVWRMLAADPAG
                     YAHDLYSALRELDSMGLRAIVVELPPEGEAWRAILDRLFRSAAGSGIADDDRL"
     gene            31402..32160
                     /locus_tag="PKV97_00525"
     CDS             31402..32160
                     /locus_tag="PKV97_00525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB04386.1"
                     /translation="MASLPIDIQVVSHGHDDCLPLLLDDIEVGRCNLFVAENLASSKL
                     QISPRADLHFVRNESEVGFAENHNRLASLGHAEFIAILNPDLRIPAGVWEGLLPYFDD
                     PGVAVVAPRVYSPRGTVEDNARFVVTPLRFLRRVMRYPDVTLDYPDITRPCEPDWVAG
                     LFMLVRRRAFEDLGGFDVGFRLYCEDVDFCIRAWLGGCKILCVPGEGVTHAARRASRS
                     NFTHFLWHLSSMFRLWRSPGWRRFVGWNRGISEV"
     gene            32663..33121
                     /locus_tag="PKV97_00530"
     CDS             32663..33121
                     /locus_tag="PKV97_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04387.1"
                     /translation="MFLNGRLEGVRVLVSVVMFAVLAFVATGCATTKPEDSDKWYQSQ
                     RQELRSKVEARWAALIKGETETAYMYLSPAQRSVVSLQQYRRTLGGAVQWRVAQVDDI
                     RYDSPTVAVVSVALTYRFASSATGNKEIESVRQMQEKWLYKDGGWWYTTQ"
     gene            33218..34678
                     /locus_tag="PKV97_00535"
     CDS             33218..34678
                     /locus_tag="PKV97_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04388.1"
                     /translation="MKLLKNTRKKVVTLAVASALGGVAMMSAPAHAMNVAQDGKGEVL
                     LFPYYTVLNGFDTILSVTNTSDRTTVFKIRWREAKNSREVRDFNVALSPHDVWTAGVT
                     VNGSGALVRTFDKSCTSPILPDGPNGSTQIDFTNAAYTNAPPFEEDGAGTAISRVQEG
                     YFEIIEMAISTVPESAIGSTNTIEFATQHVAGVPRNCATFDAAMIDPANLTNAASGSG
                     VFSSFTAPANVLIGNATLINVSNGTAFDAAPTAIQNFQDTDTIIFRPQDLSPTLGDGE
                     IVPSAFQLIDGLPTTTAIAAVAAPSVDAISVLLQANNVINEYATGEAGQSSTKTDWVV
                     TFPTKHFYTDDGVTAALPPFHEVFAPAGRSCDPIALSIYDREEKTQVTVGQSQFSPRP
                     NGPGGATLCNEVNVLTFNNSALLGSGVQLSVDTSTVGTSGWANLSLTGAPGNNGITGL
                     PVIGFSAIGRNNAADAGNNRNYGSGMPHARTIVPTP"
     assembly_gap    35227..35326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            35404..35910
                     /locus_tag="PKV97_00540"
     CDS             35404..35910
                     /locus_tag="PKV97_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04389.1"
                     /translation="MFTGTWIPEGMTDVWVVDQSETGGVGGTTVIPGCANQKSQLNVC
                     GSSLVETRNGVTTTITMAQGHTMSIRYLSEATVVTGSYFKVSSGTGAGVPAAVQVSLS
                     SQPGDFTGPSTCNKTANSGGNPLIILNGTNCPVAPNTYYYLNIKTTTACSPTNCTFKI
                     AEPASFTN"
     gene            36038..36928
                     /locus_tag="PKV97_00545"
     CDS             36038..36928
                     /locus_tag="PKV97_00545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB04390.1"
                     /translation="MKLLRPLVFVVGTTVFSQSAFSADSTFTDGESSISLSELKTELL
                     SLPPDIRAKISADKKALARFAGGVLQDRRIADAAVAAGFTKTDEIKAKIRRAERDVVV
                     QSYLTAEAARTTSEAQDFRSLAREQFEVNRAAYAVPEAVRISHILVRVDPEDDRLQER
                     EMREKAESILSKLKEGADFSELAKQNSDDKGSANRGGELPGWVEKGKTVPPFEKAAFA
                     LKPGELSDLVRTRYGFHIIKQLEYRRAANQEFGAVEAQLTNKLRTDFLKQRQSEFVAR
                     FAGTQDIQIDDATFEAVTKN"
     gene            37118..38596
                     /locus_tag="PKV97_00550"
     CDS             37118..38596
                     /locus_tag="PKV97_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNB04391.1"
                     /translation="MLFNSAEYLLAFLPICSLVYFWLNSKRLAVAGKVWLVAASLFFY
                     AWWAPAYLVLMLASVLANFWLGVRLNADASPDRSAGSRLLVVGVIFNLGLLAWFKYAG
                     FVVENANLLLGVQFPVPNIVLPLAVSFFTFTQMAYLVDCRRRIAGEYSFSNYVLFVTF
                     FPHLIAGPILHHREMMPQFESRKNLRPRPHNLALGLSLISMGLFKKVVIADTFSVWAT
                     AGFDQPISLSFFEAWISSLSYTFQLYFDFSGYTDMALGSALLFNIRLPINFNSPYKAR
                     NIQEFWRRWHITLSRFLRDYLYAPLGGNRRGRARVLANLMITFVLGGLWHGASWMFVI
                     WGGLHGAAMIIHRAWVQLGIALPQWLAWLITFNFVNLAWVFFRARDMADAKRVLEGMA
                     GINGVVLPPAFGDWLPQLKSLGISFGPILRTGNDAFQMVLWMVGAFVIVLAFRNSNQL
                     APFTGEARAIGPGRAILCGLMASASLVGILASKYSEFIYFNF"
     gene            38598..39773
                     /locus_tag="PKV97_00555"
     CDS             38598..39773
                     /locus_tag="PKV97_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04392.1"
                     /translation="MRRNSLRHGARRFVQLYFLFLVLPLATGFVPLLVYDPLQIYHKP
                     WARPQTLHSNFRLQAAAIIRREPFDGLILGTSMTENTSAEEAGRLFDESMANLSITAS
                     DFIERSLILDDIFRRRQIRHVIYTLDHVYIGTRKGYSLYPLPTFDFLYDRNPLNDIRV
                     YLNEHFAKCLLHWSGAPDCVGTAVSLDRPNAWINQTEYAVRFGGIRNWCGAKDNYQIR
                     DAYMQLRKAIDNKASGVEQSPSAEELQQRAEAAKRYVDENVLRHVRRHPETRFDFFFP
                     PYYRATYGIWHQARPIHSAVHIAMIRYLAEQAEALPNMRVSGFETESFPDDIANYKDL
                     SHYADAIDSKILAALKAESHVITRRNVDAYVDASVARAREFDLEGLAASLRNCGAGN"
     gene            39789..40625
                     /locus_tag="PKV97_00560"
     CDS             39789..40625
                     /locus_tag="PKV97_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB04393.1"
                     /translation="MNPHDNLPVTPAAMARGLWRNRDLIRQMTWREVLGRYRGSLFGL
                     AWSFFNPVLMLVVYTFVFSGVFKARWMVEGDVQASHAQFALVLFVGLILNGLFAEILN
                     RAPSLVLGNVNYVKKVVFPLEILPVVAAGAAVFHGLVSTGVLVAAILLVNGSLPWTAV
                     FAPLVMVPLVVLALGFAWFLASLGVYLRDVGQTIGILTTVLMFLAPVFYPTAALPEFI
                     RPWLLLNPLTLVIEQARDVLIWGRLPSWRGLLVYSGVAVSCAWFGYVWFQRTRKGFAD
                     VL"
     gene            40690..42012
                     /locus_tag="PKV97_00565"
     CDS             40690..42012
                     /locus_tag="PKV97_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB04394.1"
                     /translation="MSPVVISARGISKCYPIYDKPRDRLKQFLLPRLRRYAGKAPGQY
                     HREFWALRDVSFDIFRGETVGIIGRNGAGKSTLLQIICGTLTPTSGVVETRGRIAALL
                     ELGAGFNPEFTGAENVFLNGALLGLSREQVEARFDAIAAFADIGEFLDQPVKTYSSGM
                     YVRLAFAVQACVDPEILVVDEALSVGDIGFQYKCFRRMEELRDRGVTILMVTHSTGSI
                     LQYADRCIVLDGGRVSHDTPQVLEAVLAYEKGMMAQQHLPESTVSRPSVVSAVTSQEL
                     AARQASQRNAALEEVRFGSARAIIDELHVHCGASSGDPDQPVIQSGDEVVFRFRILSS
                     DWIPDVVLGVSLSKTMGGDIWGDNNLNAGVRIDLPAGPTEVDYRVRLPVSAGEYLIHC
                     GLARFDKDAREELDQRRPMRKLTVWSPRSQVGVVHAPVTVTVRSGAGT"
     gene            42009..43229
                     /locus_tag="PKV97_00570"
     CDS             42009..43229
                     /locus_tag="PKV97_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04395.1"
                     /translation="MIAVFLVPFPVRAVPAPYLWVFYKMLSAYRERVLFLIGEDYIAD
                     PQRFAAEGRWEVGEHACNDYGYRIPSRSDLEQHALRFLPADLFHGLLRECLDNPLEAF
                     RRMLTRRIPQLEAAIDQALTAEIAGGAKIEAIVTWCNCPSLTAIANRHDIRVVHLEVG
                     PLRAPEYRPTAYFDFSGVNGHTEAAARFAAIGGEPPAFDLVALRGFFTLQSESPQPAM
                     GGRVGVALQVEDDSNLVAYGNGFDNQSLIVHAKLKYGGRAKLVVRSHPGSGYWLKPGQ
                     GFELDQSPDSIAFVRGCSRILTINSSVGLESVLWGVPVTVLGDTSYAFLLEARDNRAL
                     LARLAFYLYGYLVPRQALFDLAYLRFRLTQPSEARIVAYHLQFHVPDHDAAGADLEDN
                     PWRLIASVLGDSGR"
     gene            43387..44508
                     /locus_tag="PKV97_00575"
     CDS             43387..44508
                     /locus_tag="PKV97_00575"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNB04396.1"
                     /translation="MVALTGFRGLAALWVFLYHAWGQSGMAFVGFNLGEWQVDLTPFF
                     RMGGAGVSIFFVLSGFLLALPFAEWQAGRRDKPATWLYLQHRVLRVFPAYYVQLAILL
                     AYAYHLHGASALPTPTDLLQHLFMLFVPPPLGVEPRNGVWWTLPIEFSFYLVLPLLAP
                     LLRWNRLWLLVSLSLASMVLWRHGVVTVLAGSPVPVRVIASYQLPGSMDMFGIGMLAA
                     VVLVNRDALTRWTDVFANRDRLVFLGIGLLTVAIYWLSQQASLYWADNPIFYLWTPAL
                     SLAVAGILLAGASGGRLANALLGNRVMHFMGIVSYSMYLWHFPVLVALKEAGLFTGEG
                     VVRLGSALLVGLPLTLAVSAVSYYLVERPFMRMRRRERG"
     gene            44516..47512
                     /locus_tag="PKV97_00580"
     CDS             44516..47512
                     /locus_tag="PKV97_00580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB04397.1"
                     /translation="MIDVIVPVYRGHEQTRRCLDSVLANCDVRAVEVVVVDDASPEPA
                     LAEYLDALSRSGSITLLKNETNRGFVESVNRGMSLHRDRDVVLLNSDTEVAAGWLERL
                     AACAYREPDIGTVTPFSNNATICSYPFDGWAGGVPGTLGLSRLDRLIATTNAGRHIEL
                     PTAVGFCMYIRRASLDAVGNFDVERFGRGYGEENDFSLRARAVGWRSVLAADVFVFHQ
                     GSVSFGTERTGLQRAGLQRLLEAHPRYLDALGDFSRRDPVACVRRAIDDARYALGGDE
                     RDSVICEQRTRRGATPGVSGPVQLHFTHSWDGGTNRWIADFCAYDTVRRNLVLRSVSN
                     RDFAAWRIELCDPQFLDAPLMQWDLEKPIRATDTLNLEYRLILDEIVATFGVQAMVVS
                     SLVGHSLEALDTGLPTVVVLHDLYPFCPAMFATFGQPCTTCDSTALARCMQGNPHNAF
                     WHNTDAGFWLAFRESYARRLAQPWIRIAAPSASAWGRWMQLFPGIAGRSCATIPHGLP
                     PLPGIRESTISGEPVAGQKLRLVVPGRLLPHKGLALFAAALPRLVQHADILLLGCGRF
                     GAPFHDAEGVTVVSHYAHENLVRHIREFMPDAALLLSVVPESFSYTLSEMLEIGLPVI
                     ATRVGAFAERIRDGRDGLLVAPDPDAVVAAVERLGRDGDLLAALQRAVLSRPARTASQ
                     MVADYHALLPLARSAGADDTQCALQASIALTLSIRAAKERKRLEDAGHRLRASLIEAQ
                     DHGRELGGQLAAAHRRCGEAEGRASRAVADGEAMRRSHSWQLTAPLRKVASLLRSLVG
                     SASPRAGDGLHAEEDASPVNQTGAPRIVLSDDAEVRVAARHKLREQLAIPGCSRIVLG
                     WSSGADRAFAAHFLDLAAGFNAARNDVFFLLCGVAGGDPAMADRQIGLMVATRRLFVE
                     PFEESVADTLLGADVVLLDASDPLRSILTAESSRGGPKALVIAPPSVGRTGGETAMPN
                     DVSHVAPVDAARLLSTLLDQSGPEKA"
     gene            47509..>48141
                     /locus_tag="PKV97_00585"
     CDS             47509..>48141
                     /locus_tag="PKV97_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04398.1"
                     /translation="MKNRRKPLVSPVPKSAAVDQSAVGGCLRPVSATHPLIALSGLAA
                     FFAGIVVLSLLRPFQMVAYDAALLILVIALGVFVPDMLWRRVWRNPSAGLMRSPADPS
                     WDRTITKFAGFAGSLGFIAFLYWLFPEYSTRSPFYQHFWTLLRVAIPAVLVIALPYIY
                     LVDGRMQNPHDGLWQFGRVLTFQWSDVDGRTVGQHLLGWLIKGFFLPLMFG"
BASE COUNT         8358 a        14742 c        15631 g         9310 t
ORIGIN      
        1 agcgcttgcg ccgccagcgc tggcgaggtc attgccgggc cggcggcgca agcgctggcg
       61 cacgacatca acctgctcgc cgcgctcggc gtcaagctgg tgctggtgca cggcgcgcgt
      121 ccgcagatcg acgaggagat ggcgcggcgc ggccttacac cgcgctatca ccacggattg
      181 cgggtgaccg atgccgaggc gctggagtgc gtgaaatcgg cgatgggcgt cacccgtctc
      241 gaaatcgagg cgctgctctc ccaaggactg cccaatacgc cgatggcggg tgcatacgtg
      301 cgcgttaccg gtggcaactt catcaccgcc aagccggtcg gcgtggtcga tggtgtcgat
      361 taccagtaca ccggcgcggt gcgccgcgtc attgcggacg agatccgcgc cgatctcgag
      421 caagagaatg tcgttctgat ctcgccgatg ggtttttcgc cgtcgggcga gatattcaac
      481 ctcggcatgg aggaggtcgc cgcacaggtg gcgatggcga tcaaggccga gaagctggtt
      541 tttctgtgcg acgtcaacgg cattaccgac ggccgtggcc gcctcatacc ggcgatgacc
      601 gcggacgagg tcgagaaact gctcggcggg cggcgcaagc tgtcggagga catggggctc
      661 tacctgccgt gggcggtcaa ggcggtgcgc ggcggcgtca atcgtgtaca cctcgtcagc
      721 gaagacgtcg atggtgcgct gcttctcgaa ttcttcacgc gcgagggcgt cgggtcggtg
      781 ataacccgcg atgccctcgc ccgcgttcgc caggcgacgg tcgaggatgt cggtgcgatc
      841 atcggcgtta tcgcgcccct cgaagcggac ggcacgctcg tcccgcgcgg acgcgagcgc
      901 ctcgagatgg agatcgagcg cttctcggtt ctcgaagtgg acgacgtgat catcggttgc
      961 gcggcgcttt acccgttcaa caacgacaag gcgggcgaac tcgcctgcct cgcggtgatg
     1021 cccggatacc gtcgcgaagg ctacggcgaa gcgttgatgc accacatcga attgcgcgcg
     1081 cgtaagagca gactgcagga tttgttcgtg ctcaccacgc gcaccgcgca ctggttcgtc
     1141 gaacggggat tccgcgaagc gggcatcgag gcgctgccgc ggcgcaggcg cgagctatac
     1201 aacctgcagc ggcgctcgaa ggtgttgatc aagtccctgg ccgaaaccga ctgagcggag
     1261 agcacggact tgcgtcgcgg ccccacgcat ccgttcgagc agatccggcg tgccggacgc
     1321 gcacctttgc ggctgtggcg taccctgggc ttccgctttg ccgtggtcgt catgctggtt
     1381 gtcgggatca ccgtcaccct gctcacggtg aacggcatcc gcctgctcga tgccagcctg
     1441 atacggcgcg agaaccagcg tctggaagaa atccggtcgc tgatcaatgc cacgatcgcg
     1501 ttgcatctgt cccggaacga actggcaacg gttcggcacg agatacaggc aatccgctcc
     1561 gacagcggcc tgcagtacgt cgtgcttctg gatcgtgacc agcgcattct gaccgccgaa
     1621 ggctggccgg tcgaccgccc gcttcctgcg ccggtcgagc acgtcgactc catttcaccg
     1681 ccggccggcc gcatcgatac ctatgccgcg gtgacgcgcg aaggacgtgt ctgggggcac
     1741 gtcctgttcg gggtgtcgac cgagaacctg cagcgcgtgc ggcggcagat ggtgtggcag
     1801 aacctcgcca tcggtctgat cgccgcattg cttgcgggag gggcgagctg gctgctcggc
     1861 cggctggtaa cacgtgaatt gagggcgctg cgcgccgcga cggctgcggt cggcagcggc
     1921 gagacgcaga cgcggctgcc ggtgagccgg cacgacgaga tcggcgaact tgccacggca
     1981 tttaaccgga tggccggcgc gctcaacgac cgcatggagg cactggtcaa gagcgaggcg
     2041 cgcttccacg cgatcgcgga cttcacctac ggtgtcgaag catggttcaa cccggcgggc
     2101 aggctggtgt ggatcaatcg atcgatcgag cgcgttaccg gattcacgcc ggtggaatgc
     2161 gtgctcgcgc ccgatttgtt cgacctgctc gtctattcca aggatcggcg cgcggtgcag
     2221 ggggcgttcg ccctggcgat gcagggcaag ccgacgccga atttcgaggt tcgcatccag
     2281 cgcaagaacg gcacgacgat atgggcaggg ctgaactggc agcccatctt cggcccggac
     2341 ggcgagcatc tcggcttgcg cgtatcgggc gacgacatat cctcccgcaa gcaggcggag
     2401 atcaagctcc tcgatacggt cgtcgaattg cgacgcgagc agtcgctcaa ggagtactac
     2461 ctcaagcgca gcgaggagga gcgcttccgg ctcgccgcgc tgctggatgt gctgcaactc
     2521 ggcatcctct tcgtcggccg cgatcgccgc gtcgtctaca ccaaccgggc cttcaggcgc
     2581 gtctggggtt ttgccggaac ggacgatttc gacggcatgc gcgataccgt gctgatggag
     2641 cgcatggacg ggcacctcat cgatgccggc gccttccggc ttcatgtcga ctcggtggtc
     2701 aagtcgcgcg aagtcacgcg gccgttcgag ttcacgctca gggacggacg cacgctctcg
     2761 gatgcgtcgg cggtggtttc cggtccgtct ccgatcatgt atatcggccg gatctggatc
     2821 ttcgaggatg taaccgaacg caaccgcgtc gccgagcaac tgacccacct tgccgaacgc
     2881 gacccgctga ccgggctcta caaccgccga cgctttcacg aagaacttga ccggatggtg
     2941 gccgatgcga tgcggcgtcg taccgaactc ggcgtcctgg tgctggatct cgacggattc
     3001 aagccgatca acgacaacca cggccaccat gccggcgatc aggtcctggt caggattgcg
     3061 cgcgaactgg gcagcgtcgt ccggcgcagc gagatgttct ttcgcctcgg cggcgacgaa
     3121 ttcgcgatgc tggcgcagga tacttccgaa ttcgagatga tcggcctggc gcgccgcgtt
     3181 gcggccgcga tcgcggagtt gcgcttcacc ttcggcggcg atcgcgtgtc gcgcattacc
     3241 gcgagcctcg gcatcgcgat cttcccgcag catgcggaga acggcgaaca actcgtcacc
     3301 cacgccgaca gcgcgatgta ccaggccaag agcgcaggca agaatcactg gcgcatctat
     3361 cgcggcacat cgcgcgcttc ttgagcgcga cggcagcgcg agttccgcta aaatgcaatc
     3421 gtgcgccgtg gaagtccgcg gcatcgaact tcgttgcagg agaatacaga tggctcgcat
     3481 ggtcaaatgc gtcaaactcg ggcgtgaagc cgaaggactc gatttcccca cctacccggg
     3541 cgaactcggc aagcggatat tcgagaacgt ctcgaaagag gcatggcagc aatggatccg
     3601 acaccagacc atgctggtga acgagaaccg cctcaacctc gccgacgccc gtgcccgaaa
     3661 gtaccttgcc gagcagatgg aaaagcattt cttcggcgat ggtgcagacg gcattcaggg
     3721 ttacgtgccg ccaggtgcgt aagggcgagc gcgcaaatgc aaacggggcg ctgcggcgcc
     3781 ccgttctcca tcgtggtccc ggatctcagc ccgacgaggc ctcgcgctcg atctcttcca
     3841 ggctggtgtg gcgcacgtcg cgacccttga ccagatagac gacgtactcc gacatgttct
     3901 tcgagtggtc gccgatacgt tcgatcgact tggcgatgaa caggatctcg agcgaacgcg
     3961 agatcgtgcg cgggtcttcc atcatgaagg tgatgagctg gcgcatgatg gacttgaact
     4021 cgcggtccac ctcggaatct tcccgtacca ccgatgccgc cgcctggacg tcgaggcgcg
     4081 cgaaggcatc gagcgccttg tgcagcatgt cggcggcgag cccggccacg tggcgcaact
     4141 cgatgcgcgg cacgaagccg gcctcggctt cgtgaatgcg cttggccatc ttggcaatct
     4201 tctttgcctc gtcgccgatg cgctcgaggt cggtgattgt cttgatcacc gccatcacgg
     4261 tacgcaggtc gctggccgcc ggttgacgct tcgcgatgat ctggctgcac gcctcgtcga
     4321 gttcgacttc gagcagattg acgcgattgt cgttggcgat cacctgatcg atgacgaaca
     4381 tctcgcccga ggcgagtccc tccattgcct tggcgacctg ctgctcgacc agtccgccca
     4441 tctgcagcac cttggtgcga atgttctcga gttcggcatc gaactgcttc gaggtgtgtt
     4501 cgggcatttc cattcctagt tcctccggct acagccaagc aggctaacag ccgaaggtga
     4561 cagttttatt gcggtcgttt cagttcgatg acaccctcgg cgccgccgat caacgccaca
     4621 tcggcgccgc gccgtgcgaa caggccgttg gtgaccacgc cgacgatgtc gttgagccgc
     4681 gtttcgactt cggccggatt cgcgatctgc atgccatgca cgtcgagaat cacgttgccg
     4741 ttgtcggtgg tgaacccgct gcgcagccgc ggctcgtaac ccatggcgcg cacctgacgt
     4801 tcaacgtacg cgcgcgccat cgggatgact tcgatcggca gcggaaagcg cccgagcagc
     4861 gcgacgcgct tcgaggcgtc ggcgatgcag acgaaggttg ttgccaccgc ggcgacgatc
     4921 ttctcgcgcg tcaacgcacc gccgccgccc ttgatcatcg acagccccgc gtcgatctcg
     4981 tccgcgccgt caacatagac cggcagcgca tccacgtcgt tgagatcgat caccgcgatg
     5041 ccgtgcttgc gtagccgcgc cgccgtcgcc tcggaactcg cgaccgcgcc acggatcaac
     5101 ccctccgccg cgccgagctc atcgatgaag aaattggctg tcgagccggt tccgacgccg
     5161 atgatgctgc cggccggcac atacttgatg gccgcgcggg cgacggcctg tttcagttca
     5221 tcctgggtca tggtgatcgc atcttgggtt ggcagactcc gaaaccaatt gtaacccgcg
     5281 gcctccgcaa tgccgtcgcg cgccgattgc cccgccggcg atccaggcga ataatcgtcg
     5341 cgtaacgaaa cccctcgagg agagcgaaat gggattgctg gatggattgg cggggcaggt
     5401 gctgggcagc gtgctcggtg gcggcgcgca gggcgcgcaa ggcggccagg gtgccgcgca
     5461 actgatacag atcgtgatgg gcctgatcca gaatcagcaa gggggcctgg gcgggctgct
     5521 gcagcagttc cagcaggccg gcctcggcga gcaggccgcc tcttgggtga gcaccgggca
     5581 gaatctcccg atcgacgccg gtcagctcag cgccgcactg ggttccggca ccctgggcaa
     5641 catagcgagc caactcggca tgtcgccgaa cgacgcatcc ggtgcgctgg ccagcgttct
     5701 gccgcagatc atcgaccagc tcacgccgaa cgggcaggtc accgagcaac acaactcgct
     5761 cgacggactg gccgcgctgg cggggcaatt cttccgctga agcgtgcaat ggaacccgga
     5821 tgtaaaaaaa gggcgccacc ggcgcccttt tcacgtgaaa gctgccgaag tcacttcttc
     5881 cgaatcggcg gcaagtcggt gcacacgccc tcgaagactt ccgccgccat gccgagcgat
     5941 tcgccgagcg tcgggtgcgg gtggatggtc ttgccgatgt cgatcgggtc gcagcccatc
     6001 tcgatagcca gtgccgcctc gccgatcatg tcgccggcgt tggggccgac gatgccggca
     6061 ccgacgatgc gatgcgtctc ttcatcgaag atcagcttgg tgaagccgta gtcgcagccg
     6121 ttggcaatcg cccggcccga ggccgcccag gggaacttgc ccacccccac cttgcgcccc
     6181 tgctgcttcg cctcggcctc ggtgaggccc acccaggcca cctccgggct ggtgtaggcc
     6241 acgcccggta tcacgccggc gtcgaagctc gacttctgac cggccgccgc ctcggccgcc
     6301 acgtgcgcct catgcaccgc cttgtgcgcc agcatcgggt tgccgacgat gtcgccgatg
     6361 gcgtggatgt gcgccacgtt ggtacgcatc tgcgcatcga ccgggatgaa gccgcgctcg
     6421 gtcacggtca cgccagcctt gtcggcgccg atcttgcggc cgttggggct gcgcccggcc
     6481 gcctgcagga tcaggtcgta gcgctgcgct tcggccgggg cctgcctgcc ttcgaacttg
     6541 acccacagtc cgtcggggcg ggcctcgacg gccacggtct tcgtctccag catgatctgg
     6601 tcgaaacggt gggcgttctg cttctcccac accttgacca ggtcgcggtc cgggccctgc
     6661 atcaggccgt cgagcatttc aacgacgtcg atcttcgccc cgagggtgga atagacggtg
     6721 gccatctcca ggccgatgat gcccccgccg atgaccagca tgcgcccggg cacctggcgc
     6781 agttcgagcg cgccggtgga gtcgacgatc cgttcgtctt cgggcaggaa gggcaggcgc
     6841 acggcggcgc tgccggcggc gatgatgcat ttgcggaagc ggatgatttt cttctcgccg
     6901 ctcttgccct ggccgtcgcc ggtggtgagt tcgacttcga ggtgatgtgg gtcgaggaag
     6961 ctgccgtagc cgcgcacgat gtcgaccttg cgcgccttgg ccatgccggc caggccgctg
     7021 gtgagcttgt gcacgacctt ggccttgtgg gcgcgcaggc gctcgatgtc gactttcggg
     7081 gcgccgaatt cgacgcccag ggcggcgaag tgggccgctt cgtcgatcac ggcggccaca
     7141 tgcagcagcg ccttggaggg gatgcagccg acgttgaggc agaccccgcc cagggtggcg
     7201 tagcgttcga ccagtacggt cttcaggccc aggtcggcac tgcggaaggc ggccgagtag
     7261 ccgccagggc ccgcgcccag gacgagcatg tcggtgtcga cgtcaacttc gccggcgtag
     7321 tgtccggctg cagccggtgc ggctgaggca ggcgtggatg ctggtgcccg ggccggtgcc
     7381 ggctgcgagg caggtgcagc gggcttctcc gcggtgcctg cctcgagaac caataccagc
     7441 gagccttccg agaccttgtc gccgagctgc acgcgtattt ccatcaccgt gccggcggcg
     7501 ggcgagggca catccatcgt cgccttgtcg gattcgagcg tgaccagggg gtcttccgcc
     7561 ttcacggcat cgcccggctt gacgaggatc tcgatcaccg gcacgtcctt gaaatcgccg
     7621 atgtcgggca ccttcacttc gactgtctgg ctcatttctt ttccttgatc agtttgacgg
     7681 cgtgggtcac gatcggcccg ctcgacgcgg tatcgaactc cacgcccgcc gcgacgccga
     7741 tcccggcgat ttcggctgcg gacttggtct tgtcgtaggc ggcgtacatc gcgccgagtg
     7801 cgaagttgcg gcccgagccg accgcccaga agcggtcgta ggcgaacacc tcgcgatacg
     7861 aatacacgcc gtagatgccg gtgggattgg cgatcaatgc cgtgatctgc gacgactcgt
     7921 agggatcgtc ctcgtcttcc ttgggattta ggaaggcctg ttccttgagc atcgggtgca
     7981 gcttgcggaa ggtttcgaac acctcgctgc gcgaacccag ccggaggtct tcgatcttcg
     8041 ccagcaggtg ggcaagcacc agatggtgcg ccgaactgcc gcagacaccg atccacgaat
     8101 cgccgatgtg caggatcttg tcgtgctcgg ccttgtactc ggcaccgagg cgcgtatcgc
     8161 cgaaggtggt gagcgattcg gccgagatcg cgacctggcc gttcttcttg acgacggtga
     8221 tggtagtcat ggcaatggcg ggagaagttc gggagccgcg attatcgccc gcgcccgaaa
     8281 ttctcccgga gccctcacag catcatccgc cgcatgtcgg cgagcagctg cgcgacatgg
     8341 acgttgaagc gcgttgccag cgcaccgtcg atcacgcgat gatcggcggt cagcgacagc
     8401 ggcagcatca gccgcgggac gaactgcgtg ccgtcccaca ccggcttcat cgcgctcttg
     8461 ttcacgccga ggatcgccac ttccggcgca ttgacgatcg gcgcgaagta ggtaccgccg
     8521 atgccgccga ggctggaaat cgtgaagcat gcaccctgca tctcggtcgg ggcgagcttg
     8581 ccgtcgcgcg ccttcttcgc cagttcgccg gtttcggcgg cgatctcgaa cacacccttg
     8641 cggtcggcgt tcttcaccac cggcacgacg agtccgttcg gcgtgtcggc ggcgaacgcg
     8701 atgtggaagt atttcttgta gacgagattc tcgccatcga tgctggcgtt gaactcgggg
     8761 aatctctgca acgcattcac gcaggccttg atcaggaagg cgagcatcgt cagtttccgg
     8821 cccgacttct cgttctccct gttcacctgc acgcggaagg cttcgaggtc ggtgatgtcg
     8881 gcgtcttcgt ggtaggtgac ggccgggatc atcgcccagt tgcgcgacag gttctggccc
     8941 gagatcttct tgatgcgcga cagcggtttc gtttcgatct cgccgaactt ggcgaaatcg
     9001 accttcggcc acggcagcag gtcgaggccg ccgccgagcg aggtgccggc cgcggtcggt
     9061 gcagtaccgc tcgccacctg cgccatgacg cccttgacgt agcgctgcac gtcgtcgtgc
     9121 aggatgcggc ccttcggccc gctgccggca accttcgata cgtcgactcc gagttcgcgt
     9181 gcgaacttgc gcaccgacgg gctggcgtgc gccttgccgg ccggtgcggt ggcgagcggg
     9241 gaagctggtg gtggcgcagt cggggcggcg gcaatcggct gcgccgacac ggccggtgca
     9301 actgcgggcg cattggcggc accggttgca accgcagcac cggccgcagc gagcgtcagg
     9361 atcacgctgc cttccgagac cttgtcgccg agctgcacgc gcagtgcctg caccacgccg
     9421 gcggcggggg cgggcacgtc catcgttgcc ttgtcggatt cgagcgtgac cagggggtct
     9481 tccgccttca cggcatcgcc gaccttgacc atgatctcga tcaccggcac gtccttgaag
     9541 tcgccgatgt cgggcacctt cacctcgatc gtggcggcgg ctgcggcagg ggcggccggg
     9601 gccggcgtgg atgctggcgc ctgggccggt gccggctgcg aggcaggtgc agcgggcttc
     9661 tccgcagtgc ctgcctcgag aaccaatacc agcgtgcctt ccgagacctt gtcgccgacc
     9721 ttgaggcgca gttccttcac cgtgccggca gccggcgcgg gtacgtccat ggtcgccttg
     9781 tccgattcga gcgtgaccag cgcatcttcc gccttcacca cgtcgcccgg cttgaccatc
     9841 acctcgatga ccggcacgtc cttgtagtcg ccgatgtcgg ggaccttgac ttcgataatc
     9901 tggctcattg tcgtcgtccc gttctcagac cgtcatcgga ttcggcttgt tcacgtccag
     9961 gccatacttc gcgatcgcct cggccacctt gctgcggtcg agctggcctt cgtcggccag
    10021 cgccttgagc gcggccacgg tgacccagtt gcgatccacc tcgaagaagt ggcgcagctt
    10081 ctcgcgcgtg tcggagcggc cgaagccgtc ggtgccgagc acgacgtagc ggcgcggcac
    10141 gaaggcgcgg atctgctcgg cgaacagctt gacgtagtcg gttgccgcaa tgaccggacc
    10201 gcgcgtgtcc ttgaggcagg attcgacatg cgagagtttc tgcttttcgg tcgggtgcag
    10261 caggttccag cgcgcgttcg cctggccctc gcgcgccagt tccgtgaagc tcgggcagcc
    10321 ccacaggtcg gattcgacgc cccagtcctt gcgcagcatg tcggcggcag cgatcacctc
    10381 gcggaagatg gtgccgctgc ccagcaattg cacacgcggg ccgttgctgg ccgcgccctg
    10441 gcggaacgag tacatgccct tgaggatgtc ggcctctgcg ccggccggca tctcgggatg
    10501 ctcgtagttc tcgttcatca ccgtcaggta gtagtagatg tcctcctgct cggcgtacat
    10561 gcggcgcagg ccgtcctgca cgatcaccgc gagttcgtag gagaaggtcg ggtcgtagct
    10621 gacgcagttc gggatcgcgc ccgaccacag gtggctgtgc ccgtcttcgt gctgcaggcc
    10681 ttcgccgttg agcgtggtgc gcccggcggt gccgccgacg agaaagccgc gcgcgcgcat
    10741 gtcgcccgcc gcccagcaca ggtcgccggt gcgctgcagg ccgaacatcg aatagaagat
    10801 atagacggga atcatctgca ggccgtgcgt cgaatacgac gtcgccgcgg ctatccagtc
    10861 ggccatcgcg ccggcctcgt tgatgccctc ctgcaggatc tggccgtcct tcgattcctt
    10921 gtagaacatc agctggtcgt ggtcctgcgg cagatacttc tgtccttgct ggttccatat
    10981 gccgatctgg cggaacatgc cctccatgcc gaaggtgcgc gactcgtccg gcacgatcgg
    11041 cacgatgcgc ttgccgacct gcttgtcctt gagcaggatg ttgaggatgc gcacgaaggc
    11101 catcgtggtg gacacttcgc ggccttcgcc gctcgccttg agcagcggct cgaaggcgga
    11161 cagcggcggc accgcgaggg catcggcctt gcgccggcgc gccggcaggt agccgccgag
    11221 ttccatgcgc cgcgcgcgca tgtattcgag ttccttcgag ccttcgggga acttgaggaa
    11281 caccaggtca tcgacctgct ggtcggtgag cggcagcttg aagcgatcgc ggaatttcat
    11341 cagagaatcg cgcgacatct ttttcgcctg gtgggcgatg ttcgccgcct cgcccgacgc
    11401 gcccatgccg tagcccttga tggtcttggc gaggatcact gtcggtgcgc cctggtggtg
    11461 gatcgcggcg tggtaggcgg cgtagatctt gtgcacgtcg tggccgccgc gattgaggtt
    11521 ccagatctcg tcgtccgacc agtcggccac cagcgccttg agttccggcg tgttgaagaa
    11581 gtgctcgcgc acgaaggcgc cgtccttcgc cttgtaggtc tggtactcgc catcgacggc
    11641 ttccatcatg cgcttcatca ggatgccctt cttgtcgcgc gcgagcaggg cgtcccagtg
    11701 cgtgccccag accagtttga tgacgttcca gcccgagccg cggaactcgc cttcgagttc
    11761 ctggatgatc ttgccgttgc cgcgcaccgg accgtcgagg cgctgcaggt tgcagttgat
    11821 gacgaagacg aggttgtcga gcttctcgcg cccggccacg ctgatcgcac cgagagactc
    11881 gacctcgtcg gtttcgccgt cgccgaggaa tgcccacacc ttgcggccct cggtcttcgc
    11941 caggccgcga tcatgcaggt acttcatgaa gcgcgcctgg tagatcgcca tcagggggcc
    12001 gaggcccatc gacaccgtcg ggaactgcca gaagtccggc atcagccacg gatgcggata
    12061 gctcgagatg cccttgccgt cgacttcctg acggaagttg ttgagctgct cgtcggtcag
    12121 gcggccgagc atgtaggcgc gcgcgtagat gccgggcact gaatggccct gggcgaagat
    12181 caggtcgccg ccgtgattgt ccgacggcgc gtgccagaag tggttatagc cgacgtcgta
    12241 gagcgtcgcc gccgaggcat acgaggagat gtggccgccg acgttggttt cctcgttggc
    12301 gcgcagcacc atgaccatcg cattccagcg cgtgtagtcg cggatcgtgg tctcgatttc
    12361 ctggtcgccg ggaatgcgcg cctgctgctc gaccggaatg gtgttgatgt actgcgtggt
    12421 ggccgagaac ggcaggttga ggccgcgcag ccgcgcatcc tcgatcatgc gttcgagcag
    12481 gtaatgggcg cgttccggcc cttcggccgc catcacggcc tccagcgcgt cgagccattc
    12541 ctgggtttcg gcggtgtccg gatcggactg cagcagcaca ttgggtagcg cggacatcga
    12601 agcgtctcct cgtcagtgat gtggcacgga ctacagcgcc gcgacgcatc cggggcgcct
    12661 tttgggcgtc ggatcgtgcc gcgaacgcaa gcttggtggc gctttgtgcg ccgggctccc
    12721 gtttccgtgc aggtgagcga agcctagtac ggcttggcct gcaggactaa ggattttgcg
    12781 cattgtaaaa tctgattgcg tttagcgcaa ttgtggaaat ccccagtatg ggtcgttcgg
    12841 gcggatcggg agcggtcggg cgttgatggt tcgcgcgggg ccggcccgag gcttgcgccg
    12901 cggatcgact gcgtcacggc agatttggag acgggcaggg aaacccgggc ggtcggcgcg
    12961 tcagatacag cgccaggcgg gggcgagtcg gtccggacgc cggcgcaagg tatccatgac
    13021 gatcagcgtc tggacgatca agagtccttc gagcggcttg cggtagcggc cttcgttgat
    13081 ggccagcaga gatacggcct gcacgcaaaa ccaggtcgca atgatggcgg gaacgagcgg
    13141 ccgcgccagc gttgcgcgcc acgtgaccgc gccgacgacg agcagcgcga cgaacagcgg
    13201 cgcccagatc cagcgcagcg aactggcgag ccgggccgtc cagtgctgct ggttgctgtc
    13261 cggccaggtt tcgccggcca tcaccagggc gaggttctcc agccgaaagc gcacgagatc
    13321 ctccgctgat gcacgggtgc gttcggcagc ggcctgccag tcgcgcgcgc cctgcttcag
    13381 atcgacgttg acttcgaccg cgccctcgcg caccggcgtc cagtcggtga gcggctcgaa
    13441 ctgcttgtaa ctgagcgacg gcgatcggaa gccataggcc cacgatccat cgccccaacg
    13501 gaatttcagg ttgatttcgc gtttgccgct cgccgcgtaa atctcgttga gccagccaca
    13561 gccgaacggc gaccagaatc cgaaggtagt ctggttgcgg taggcgagcg ggccaaggat
    13621 gaccacggcg atcaatgtcg agcccgcagc tgcgcgccac ttgagcggat gccgatacca
    13681 aacgagaaat ccggcgacga cggcgatcgg tgccgcgatg ccacgcgtca gtgcggtgaa
    13741 tatccacagt acagccatca cgcagaacga gacggtactc tgcttcctgc gcgcccgcgc
    13801 cgttgcccac aggctcgcgc cgagcagtgg cagaaacagt gtttcggtca tgaaatagcc
    13861 gaagatgtcg aaccatgacg gcaaccaggc gaacagtgcc catcccagca gcgccagccg
    13921 gcgcgatgac agcatctcgc gcaggaagcg gtaccacagc cacggcgtga gcagcgacat
    13981 ggttgccgca tagaccgcga tcaacgcggg gtgcccgagc gtccacttct gcacgatcga
    14041 cagccagacc tggaagaacg gtgggtcgag cagcgccatc ggccccgggg cgagcggcat
    14101 gcgcgcgttg tcccagtgtc gcagcgggtc gctgaaaagg tggaatgcag ggttgtggta
    14161 gaaggggtcg agcagccgca gcaggcagcc agccagtatc agtgcaccca gcacgacgcg
    14221 cggccagtcc caggcttcat cacggggcaa cagtggcgct tggatcgggg agcgatgaaa
    14281 catgggtcga tgctcgggag cgggccggag cagactcgca tatcatggcg ccggctggcg
    14341 aatgcaaatt taacggcttc gtggttttac acaattaagg gctttgtcac ccgtgatatc
    14401 catcatctgt cctttgtaca acgaggaaga tgcggtcggc cccttcctgg aacgcctgat
    14461 tccggtcatg gagtcggtcg gcgaatcctt cgagatcctg ttcgtcaatg acggcagcaa
    14521 cgaccgcacg cttgagtgcg tgcttgcagc ccggcaggcc gacggacgga ttcgcgcggt
    14581 cgacctgtcg cgaaacttcg gcaaggaggc ggcgctcacc gccgggctgg atttcgcgcg
    14641 aggcgacgcg gtgattccca tcgacgtcga tctgcaggac ccgcccacgg tcatcgtgga
    14701 gttcgtgcgg aaatggcgcg agggattcaa gatggtcgtg ggcaagcgcg gcgaccgacg
    14761 cgccgattcg tggccgaagc gcgtctcggc cggcttgttc tatcggatcc acaaccgcat
    14821 ctccgatctg cagattcccg aggacgtggg cgatttccgg ctcatcgatc gtagcgtggt
    14881 cgatgcgttg cgccagtttc cggagcggcg ccggttcatg aagggactgt tcgaactcgt
    14941 cggtttcgat actgccgtgg tgacgtatgc gcgcgaatcg aggctggctg ggacatcgaa
    15001 gttcagcggc tggaagctct ggaattttgc gctcgagggc atcacctcct tcagcaccgc
    15061 gccgctgcgc atctggacct acatcggctt gctgatgtcc ctggcggcct tcctgtatgc
    15121 ggcgttcatc gtcatcaaga cgctggttct cggcatcgac gttccgggct atgcgtccct
    15181 gctgaccgtc atgctgttga tcggcggcct gcagctcgcg ggactcggtg tgatcggcga
    15241 gtacatcggc cggctctacc tcgaatccaa gcggcggccg atctatctcg tcaagggcgt
    15301 gttcggggaa gccaagcggc cagtgaccgc aggcgaagcg cccttgccgg gcgattgatg
    15361 cgggcggctg gccccagcac ggatcccctt gctaacatgg ccccgcgcgt cgggctggca
    15421 agggtgccgg ggacgcgtac gcatagatct tagggtgagc cctaatggcg cagtccgggc
    15481 gcgcggacga taaagcgcga ttccggtcac gagtgctggt tctgacatgg atagatcgcc
    15541 tttccatcgt gtgattcccc gcgtgtcgag ctcgcgcgaa tggctgtggt atttgcccta
    15601 catggcggtc gggctgttcg cgctggcgat gttcgccctg gtctgggggc tgcagaagcg
    15661 ggaaatcgat ctcgagcgca atgcgctcgc gcgcgacgtg caatgggccg agcagacgat
    15721 gcgcctgcac ttgcaggcca accaggagtt cctcgtccag ctagcgcggg atttcgccca
    15781 gggtgcgctg accgaaccgc agttcagtgc gaaaacgcga cagcatgtcg ctggcaatcc
    15841 cgaattgacg agcgttgcct gggtcgatta cgacgaaaag atcatctggg cggcgccttt
    15901 tgacacggcc gactggatgt cgggcgagcg cctcaatgcc aaccaggcgc tggcgttcat
    15961 caacgcgcgc gaagcggccc gtccgacata cggcgagccc tacgtcgatg accgcggcaa
    16021 gaaactgctc gacatctatg tgccgctgtt cgacgggcgg cagcatgtcg gcgccatggt
    16081 cggcgtgtac tcgatagaag gcatgctgcg ccacctggtg ccgacgtggt tcaccgagaa
    16141 gtaccagctc tcgatggtca tcggcgagga caaggttctc gcatccagca gcgcgacgcg
    16201 accgatggag gacgcgatcg cttacgtgat tccgatcgag ccgatcggcc atgacctgaa
    16261 actgcgcacc gccggctatc gcaccacttc gcagctgtcg cgactggttc ccgcggttct
    16321 catcgtgggc ctgtcgctgg tcatcgtcgg atcgctctgg gcgttgcgca tgcacctgcg
    16381 ccgccgcgtg caggtcgaga aggagcgcga ccgcctgttc aacctgtcgc tcgacatcct
    16441 gtgcattgtc gggctagacg gtacattccg gcgcatcaat ccggcgttcg agcgcattct
    16501 cggctatccg ccggacgaac tcgccggcac gccgctgctc gagctggcgc accccgaaga
    16561 catggcggcg accgtcgagc acctgcgcgc cctctcggcc ggcgtgccgg gcactttcga
    16621 gaaccgcgtg cgggcggcgg acggccgcta ccgctggctc gcgtggagcg ccaacccggt
    16681 gcccgaggag aagctgatct atgccgtcgc gcacgatgtc acccagcgcc gcgccaacga
    16741 ggaggcgctg cgcgccgaga cgtcgttccg caaggcgatg gacgagtcgc tcgtatcggg
    16801 tctgcgggca atcgacatgg aggggcggat catctacgcc aacgcggcgt tctgccgcat
    16861 gaccgggttc tcgcaggcgg aactcgtcgg caccgcgccg ccgtttccct actggcctcc
    16921 cgaggatctc gatgtctgcg agcgcaatct ggcgctgacc ctgtcgggcc acatcccgtc
    16981 cggcggcttc gagacgcgcg tcaagcgcaa gaatggcgag cgtttccatg cgcgcttcta
    17041 tgtctcgccg ctgatcgaca gcgaaggcag gcagaccggc tggatggcat cggtcaacga
    17101 catcaccgaa ccgcgccgtg cacgcgcggc gctcgaggcc tcgcaggaac gcttcattgc
    17161 cgtgctcgac ggcctcgatg cggcggtgtt cgtggccgac gccaataccg acgaagtgct
    17221 gttcgccaac cggaccttca aggcgatcta cggtttcgat gtggtccggc gcagcgcgga
    17281 ggaagtcgcc cccggactgt cgccgcagcg caaccagtat gcggtcgatc cgcgcgcgct
    17341 gtcggccgac gacctgccgc gcgagctgtt cgacggtgag gtgcagaatc ccctgtccgg
    17401 ccgctggtat cacctgcgcg aacgggcgac gcgctgggtg gacgggcgtg tggtgcgcat
    17461 ggcgattgca accgacatta ccgaccgcaa gctgatcgac gaagtgaacc tgcagcagca
    17521 gcagcgtctg caacagacgt cgcggctgat cacgatgggc gaaatggcgt cgagcctcgc
    17581 acacgaactg aaccagccgc tttctgccat cgccaactac aacatgggtt gcgtcaaccg
    17641 gctgcagtcg ggcgagtacc gcgtcgagga catcctggcg gcgatgcaga agtccagcca
    17701 ccaggcggag cgggccggca agatcatccg gcgggtacgc gagttcgtac gcaagagcga
    17761 gccgcagcgt gcgccgtgcc ggctcgccga gatcgtcgag gatgcggtcg ggtttgccga
    17821 gatcgatgcg cgcaaggccg gcgtcagcat cacgatcgac gttccgcgcg agctgccgct
    17881 ggttttcgtc gaccgcatca tggtcgagca ggtcgtgttg aacctggtga agaacggcat
    17941 cgaagcgatg cagggcccgg tttccgatcg gcgcgaactg gacgtggagg cacatgtggc
    18001 ggacgatcgg catgtcgaac tgtcggtcag cgaccgcggc catggcctgc ctgccgacgc
    18061 gggcaacaag ctgttcgtgc ctttctacac caccaagacc cagggcatgg gcatgggcct
    18121 gaacatctgc cgctccatcg tcgagtacca cgacggccgg ctgtgggcgg aagatcgccc
    18181 cggcggcggc agcacgttcc gcctgaccct gccgatccac ctggaggaat gacgatggtc
    18241 gatgtgcaat ccgggcgcgc aggcggcgtg cagtgcgtgc acctggtcga tgacgacgag
    18301 gccctgcgcg attcgctggt gtggcttacg gaatcgcagg gattcaaggt cgccgcctac
    18361 gattcagccg aagcctttct tgccgcatgg tcgccggata tggcggggtg cctggtgctc
    18421 gacgtgcgga tgcccggcat gagcggtctg gagctgtacg agcggctcac gcagatgcac
    18481 tcgacgctgc cggtgatttt catcaccggc catggcgacg tgcctatggc ggtttccgcg
    18541 ctcaagaagg gggccgtcga tttcatcgag aagccgttca acgacaagga catgctgcgc
    18601 ctgatcgaaa cctgcctggc gctcgaccgc gagcagcgcg aaaagcgctc ccatacggcc
    18661 gagatccgcc gccagctcga gcaactgacc cagcgcgagc acgaggtgct cgacctgatc
    18721 atcgccggaa agctcaacaa gcagattgcc gacgtgctct cgatcagtat caagaccgtc
    18781 gaggtccatc gtgcgcgggt gatggagaag atgggcgcgc gaacgctggc ggaccttgtc
    18841 cagcgcgtgc tgtcggccgg cgcgggctga tccggggggc ggcggcctgt tcggatcgcg
    18901 cgtgttgccg ccgcaggtca gatttcgacc tgggtaccca tttcgaccac gcgattggtg
    18961 ggaatcttga aaaagtccgt cgcgctcatc gcattgcgcg acatggcgca gaagagccgt
    19021 tcgcgccacg gcgccatgcg gcgcttcagg ctgggtatca cggtctcgcg cgacacgaag
    19081 aacgaggtcg acatcatgtc gaacgtcagg ccatgcggcg cgcagagttc gagcgcggcc
    19141 ggcacgtcgg gcgcctgcat gaagccgtag tgaacgatca cgcggtggaa gcccttgccg
    19201 agatccgtga cgtcgacgcg ttcctcggcc gaaacgtacg gccggtcttc ggtgaccacg
    19261 gtcagcagca cgttgcgctc gtgcatgacc tgattgtgct tgacattgtg caacagggca
    19321 gacggcgcgc cctcgtcgtg gccggtcatg aacacggcgg tgccgaacgc gcggttgggg
    19381 cagtgctcca gcccttcgat caccgcttcg agcggaatgc tctggcgctt catttcgttg
    19441 aacaggaggc gccggccgcg cttccacgtg gtcagaatgg cgaacgagac cagtcccatc
    19501 gccaggggaa accagccgcc ttgcggaatc ttgatggcgt tggcgaagaa gtaggcgagg
    19561 tccacgatca ggaagccgcc gatcgcgacg atggccagcg ccttcggcca gcgccagccg
    19621 agaatgatga cgaagccgat cagcacggtg tcgatggtca tcgtgccggt caccgcgatg
    19681 ccgtaggccg cggcgaggtt gctcgagttg ccgaacccga tcaccagccc catcaccgca
    19741 aggtagagcg tccagttggt gaagggcacg tagatctgcc cctgcgcctt gtccgaggtc
    19801 tggcggatct ccatgcgcgg gaggaatccc atctgcaccg attgccgtgc cacggaaaat
    19861 gcgcccgaga tcacggcctg cgaagcaatc acggcggctg ccgtggcgag aagcaccatc
    19921 ggcatttgcc cccactcggg ggccagcagg tagaacggat tctcgatcgc ctgcggattc
    19981 tcgagcagca gcgccccctg gccgaagtaa ttgatcacca gcgccggcag cacgaaaccg
    20041 aaccaggtta ggcgaatcgg aaagcggccg aaatggccca tgtcggtgta cagcgcctcg
    20101 ccgccggtca ccgcgagcac caccgtgccg agggcgagaa acgcgacgtg aaaatccgtc
    20161 acgaggaaat ggagtgcgta cgccggattc agtgccgcga gcacgtgcgg agaccggatg
    20221 atcgcctgga agccgagcaa cgccagcacg ccgaaccacg cgatcatgat ggggccgaag
    20281 gccatgccga cgacaccggt gccgcgcttc tgtaccacga acagcgcggt cagcacgccg
    20341 gctgtgatcg gcagcacgaa cttgctcagc tcgggctcga cgacgttcag tccttccacc
    20401 gccgacagca ccgagattgc cggcgtgatc atgctgtcgc cgaagaacag tgcggcggcg
    20461 aaaatgccca gcatggtcac cgtccagcgc attcgcttgt tggcgcgggt gagttcggaa
    20521 accagggcga gcagcgcgag cgagccgcct tcgccgcggt tgtccgcacg catgatgatc
    20581 gcgacgtact tgagtgacac cagcgccatg atggtccaga acatcagcga caggacgccg
    20641 aggatgttcg cctccacgac ggcgatcggg tgatgaccgg cgaaggtttc cttcatcgcg
    20701 tagagcggac tggtgccgat gtcgccgtaa acgacgccga tggcgccgat cacgagcgcc
    20761 gacaggttct tgtttgaact cgattgcgac atggtcgcag ctccctgaat cgcaacccga
    20821 ccgttctgcg cgtcctgcgc cgacagcttc tatgttgcgg tgcgattctg acgcatccgg
    20881 ggtccgatgg ggcgggtttc agcgaattgc cgcggtctgg tcgcgccagt cgtgcgcaaa
    20941 tgcaacggct gcgcccatga atgtgccgaa atcggcttcc agttcagcgt aatgcgacag
    21001 caattcctcc acgccgccgg cgagcgggtt ggctcgggtc agccgatggc gggaaatgcc
    21061 gtccagcgcc gcgccgatgc cggcggcgtc ggcgtaggaa accagccagt tctgctcgcg
    21121 catgtgcggc agcaagccgc gcagtctggg cggcaggagc gcggcgtggt cggccagcag
    21181 cgcgtaggtg tcggcggcga actcgtggag cgtctgatcg ctgtagtgcg gccactggag
    21241 ggcgaggaaa tggtcgtaga acatgtcgat catgatcccc gcgtagcgcc tgcgcagtgc
    21301 gctcacgcgg ccgcggctgg cctggaacgc gggatgcgtg tcggcgtagc ggtcgatctg
    21361 ccggtgcagg gcaattccgg cgacgacgcc cggcggaagg cgcgggtcga acaaagcggg
    21421 cgtgcccttg acgaaatcgc ccagcaggcc gcccaggcgg gattcgggat cgggcccgga
    21481 gagcagtgcg tgggccaggt agttcatgcg aggcgtcgaa atcgtgcgaa gggaattgtg
    21541 taagcaaagt ttgccacacc gtccggcgcg cgacgcgggg gctatcatgc aaatgagagt
    21601 aggtcgtttg catgcgctgc ctgcgacggt acgtcgcggg ttgctgcaag gaggaagcga
    21661 tgaggacgag tcgcaagatt gtggtgggtg tcgcgctggc cctggctgcc gggatggtgc
    21721 aggccgaccg tggcgggcgt cacggcggtc attggcacgg gccgcgcgtc ggtgtggtga
    21781 tcggtgcgcc gtttggatgg tatggaccgc cgttctggta cggcccgcct tactggtacg
    21841 agccgccggt ggtcgtgcag tcggcgccgc cggtgtatat cgagcgcggc gccgacgtcg
    21901 ccggcagcga gccggaaccg tattactggt accactgcgg caagccggaa ggctactacc
    21961 cgtatatcaa ggattgtccg ggcgggtgga agaaggtcgt gcccacgcca ccgtccaact
    22021 gaaaggaagg caggaacaac atgaagcgcc tgatgatcgt ggcggccgcg atggtgctcg
    22081 ccggctgcac cacactgccg acagggccga gcatgctggt gttgcccgga acgggtcgca
    22141 atttcgacga gtttcgcgcc gacgatgcgc aatgccgaca gtatgcgcag gatcaggtgt
    22201 ccggcgtgac ggcggaacgc gcatcggtcg acgcgggtgt caagagtgcc gtagtcggca
    22261 cggtgatcgg tgcggcggcc ggcgcggcca tcggcgggca tcgcggggcc ggcgtggggg
    22321 ccggaaccgg gttgctgatc ggcagcgccg ccggggcgga atcggccagc ctgtcgggtt
    22381 acggcgcaca gcggcgttat gacaacgcat ttattcagtg catgtatgcc cacggccatc
    22441 gcgtgccggt gagcggcaac tattcgcccg agcggccgcg ccagccctac tatccgccac
    22501 cgccgccgcc cgggtaccag cagaagtagg ctccagccaa cggccggatt tccccggccg
    22561 ttcttttgtg ccatgcctgg agagcgcatg aataggcgat ctcggggtat gcacctctgg
    22621 aaagcccgcc ctgtctgcct ttcagggccc cgtgtttttc gccctgccgc gtgcggtcgg
    22681 gcgtttccac gatggtcagc ccggttcgct tcgactaaag tccacccaaa agcgaacccg
    22741 ccgatggagg agatgcagcg ggtcgcgcac gcggagcgcc gcgtccggac cgaagccggc
    22801 ttcggccccg gctgcggcga ttctgtcgcg tcccccaacg agatccggct gccacgccag
    22861 tgcgtcgcgg ccgtgcatgc cgttcgacaa gggaggatgc atgttccagg ttcgcataca
    22921 cgggcgtggc gggcagggcg tcgtgaccgg cgcagaaatg ctctcgatcg ccgcgtttct
    22981 cggggggcgc cacgcgcagg cctttccgag cttcggttcc gagcgcaccg gggcgccggt
    23041 ggtcgcgttc tgccgcatgg acgacaagcc gatccggttg cgcgagccga tcatggagcc
    23101 cgatgcgctg atcatccagg atccgacgct gctgcatcag gtcgacctgt tcgccgggct
    23161 caagcgcgac ggctacatcc tgatcaacac caaccgcagt ttcgacgaac tcggcctcgg
    23221 cgaattcgtg aagggctttc gccacgatcg cctgctcacc gtgccggcga ccgagattgc
    23281 cttgaagcac gtcggccgtc cggtgccgaa cgtgccgctg ctcggcgcct tcgcggcgct
    23341 cggcgggctg gtcacgatcg atgccgtgca tgcggcgatc cgcgaaaagt tctccggcaa
    23401 gcttgcagaa ggcaacgttg ccgcagccga tgcggcctgc cagttcgtgc gcgaagaaga
    23461 agaggaggct gcgcgccatg ctcaagcaaa ctgaaggatc gcgcgccgtc gccgaagctg
    23521 tcgcactgtg ccggccggaa gtgatctgcg cctacccgat cagcccgcag acgcacatcg
    23581 tcgaggggct gggcgagatg gtgaaatccg gcgcgctgtc gcgctgcgag ttcatcaacg
    23641 tcgaatcgga gttcgccgcg atgagcgtgg cgatcggtgc ctccgctgcc ggcgcgcgcg
    23701 cctacaccgc gaccgcatcg cagggtctgc tgttcatggc cgaggcggtc tacaacgcat
    23761 ccggccttgg attaccgatc gtgatgacgg tggccaaccg cgcgatcggc gcgccgatca
    23821 acatctggaa cgaccattct gattcgatga gccagcgcga ctgcggctgg atccagttgt
    23881 tcgccgaaac caaccaggag gcgctcgatc tgcacatcca ggccttccgc ctcggcgagg
    23941 aactttcgct gccggtgatg gtgtgcatgg acggcttcat cctgactcac gcctacgagc
    24001 gtgtcgacat gccgacgcag caacaggtcg acgcctacct tccgccctac gagccgcgcc
    24061 aggtgctcga ccccgacgcg ccggtgtcga tcggcgcgat ggtcggcccc gaggcgttca
    24121 tggaagtgcg ctatctcgcc catgccaaac agatgcaggc gctcgaactg attccgcagc
    24181 atgcggccga gttccgtgcc atcttcggcc gcgattcggg cgggctggtg cggccctacc
    24241 gctgcgagga tgccgagacg atcgtcgttg ccctcggctc ggtgctgggc acggtcaagg
    24301 acacggtcga cgaactgcgc gactcgggcg agcgcatcgg cgtgcttggc atcggctcgt
    24361 tccggccgtt tccgctcgct gcggtgcgca atgcgctgga agccgcgaag cgcgtggtgg
    24421 tgctcgaaaa gagcctcgcc gtcggcatcg gcggcatcgt gtcgaccaac gtgcgcatgg
    24481 cgctctccgg cctgcacctg catggctaca cggtggtggc tggcctgggc ggccgggcga
    24541 tcacgcgcgc aagcctgcac cgcatgctgc gcgatgcgat cgcggaccgc ctggagccgc
    24601 tgaccttcct cgacctcgac tggaagctcg ttaacaacgt gctggcgcgc gagaagcagc
    24661 aacgtcgctc gggaccgatc gccgaaggca tgctgcgcga cgttggcgtc gtcgcatcgc
    24721 gagtgggctg aggagcgcgt gaaatgagca cacagaccat caagttctac cagaccggca
    24781 ccttcaccgt cggcaaccgg ctgctcgatc cggcgcagcg cagcgtgcag gccacggtgg
    24841 cgcgcaccaa ctcgatcaac tccggccatc gcgcctgcca gggctgcggc gaggcgctcg
    24901 gtgcacgcta cgcgatcgat gcagcgatgg cggcgacgga caaccgcctg atcgccgcca
    24961 atgcaaccgg ctgcctcgaa gtgttttcga cgccgtaccc ggaaacctcg tggcagatcc
    25021 cgtggatcca ttccctgttc ggcaacgccg cggccgttgc cacgggcatg gccgcggcgc
    25081 tcaaggtgaa gcggcgtggc gacgtgcgcg tggttgcgca gggcggtgac ggcggcacca
    25141 cggacatcgg cttcggctgc ctctccggca tgttcgagcg caacgacgac gtgctctaca
    25201 tctgttacga caaccaggcc tacatgaaca ccggcgtgca gcgttcgtcc gccacgccac
    25261 ctgcagcgcg caccgcgacg acgatgtcgg tcggcgcgca cccgggcaac gtgtttggtc
    25321 agggcaagga tgtgccggca atcgccatgg cgcacggcat accctatgtc gcgacggcga
    25381 ccgtcgccga cctgcgcgac ctcgaggcga aggtggagaa ggcgatgtcg atccacggcg
    25441 cgcgctacat ccacatcctc gtgccctgtc cgctcggctg gggaacggcg tcgcacgaga
    25501 cgatacgcat cgcccgcctc gcgcgagaaa ccggcttgtt cccggtgttc gaggcggcgt
    25561 tcggcgaagt caccggcgcc tcgaagatcc gccatcgcct gccggtcgag gattacctga
    25621 agccgcagcg gcgtttcgcg catctgtttg cgccccgcct cgatgtcgag acgattgcgc
    25681 gaatccagca gatggcggac cgcaacatac gcaagttcgg attggtcgag ggagaagtgc
    25741 aatgagcagg gtcgtgcaaa ccaagccgtt cgccatcacg ctcgacgtcg gatcgtcgct
    25801 tgccaaccat accgggtcgt ggcgcacgat gcgtccgatt tacctggatc gcattccgcc
    25861 gtgcaatcac cagtgccccg cgggtgagga catccagggc tggctgttcc atgccgaaag
    25921 cggcgattac gagaaggcct ggcgccatct ggtgcagaac aatccgtttc cggcgatcat
    25981 ggggcgggtc tgctaccact cctgcgaatc ggcctgtaat cggggcaggc tcgatgaggc
    26041 cgtgggcatc aactcggtcg agcgcttcct gggcgacgag gcgatccggc gcggctgggc
    26101 gttcgactca ccggcggcgg cgtccggcaa acgggtgctg gtggtcggcg ctggtccgtc
    26161 cggtttgtca gctgcgtacc acctacggcg gttgggccac gctgtgacgg tgcgcgaggc
    26221 agggccgctg ccgggtggga tgatgcgctt cggcattccg aagtatcgcc tcccgcgcga
    26281 cgtgctggat gccgaggtca agcggattac cgacatgggc gtggtgatcg agtacaacgc
    26341 cagggtgggc gacatcctgg cctcgatgcg cgaaggcttt gacgcggcct tcctcgcggt
    26401 cggcgcacat atcgccaagc gggccttcat ccccgccggc gattcggcca aggtgctcga
    26461 cgccgtgtcg gtgctgcggt cgctcgaggg cgaggaacag ccgatgctcg gccgccgcgt
    26521 ggtggtctat ggcggcggca acacggcgat cgacatggcg cgaaccgcga agcgcctcgg
    26581 cgcgacggag gcgatcatcg tctatcgacg cacgcgcgaa cggatgccgg cacacgacat
    26641 ggaggttgag gaagcgctgc aggaaggcgt gatggtcaag tggctctcga ccatccggca
    26701 ggctggtgaa tcgagcctga cgatcgagaa aatgcgcctg gacgagaaag gcaacccggt
    26761 gccgaccggc gaattcgaaa ccctggaagc cgattcgctg gtgcttgcgt tgggacagga
    26821 tgtcgatctg tcgctgatcg agcgggtgcc cgggctggaa attcgcgatg gtgtggtaca
    26881 ggttgaccgg cagatgatga ccgcctaccc gggaatattc gcaggtggcg acatggtgcc
    26941 ggcggagcgc aacgttaccg tggccatcgg acacggcaag aaggccgcgc gcaacatcga
    27001 cgtctggttg cgcggcgcga cccgtgaagc gccgccgagg catgaactgg ccgacttcga
    27061 gaaactgaat acctggtatt acagcgatgc gccgaaaacg gtacgcccgg tgctcgatat
    27121 catccgccgc caatcgacct tcgacgaagt gctgggcggg ctcgacgaaa gcaatgcact
    27181 ctacgaggcg cgtcgctgcc tgtcctgcgg caactgtttc gagtgcgaca actgctacgg
    27241 cgtttgcccg gacaacgcaa tcatcaagct cggaccgggc aggcgtttcc gctttaacta
    27301 cgattactgc aagggctgcg gaatctgcgt gtccgaatgt ccctgcgggg ccatcaagat
    27361 ggtgccggag gatatctgac cgccagaaag aaaaaagccg cccaagcttg cgctgggcgg
    27421 ctggaacccc ttgcgtagca aggaggaggt cagccggtgc cgaacacctt caaggcattc
    27481 gcgattgatt cgattgtaga cgaaacccgg tacggaagaa aacagtttcg atatcgctcc
    27541 ggatgtccaa agaacacggt gcttcgcctg gaattggagg aaagtgaagc agtattcatg
    27601 cctcgttgga ggccggtggt actttgttgc ttgattggca aagaaaacct cgagtcgacg
    27661 tcaggaatcg ggactgcctt gaagggtttg agggtcccca tctggttccg ggttgtcgtg
    27721 aaatggagac gcatcgtctc ggacgttgcc gcaaattgac gctccggtct ggcaacagca
    27781 ccgtcggcga gcgcccttgt ggcgacgcga aacgctgaaa tgctagaatt ccgccttttt
    27841 tcgggacggg ctcatgtatc ggcgagacga gatatgcagg cgcgtttgat cgacgggaat
    27901 gccctgtctg caaccctacg gtcgcatttc gccgagcgtg cccaaaagct cgccgggcgg
    27961 ggcattaccc ccgggctggc ggtcatcctg gtgggggaaa accccgcgtc cgctgtctac
    28021 gtgcgcaaca aggttgccgc gtgcgaaaag gccggaatcc gttccctccg cttcgattat
    28081 tccgagtctg tcgatccgca agaggttttc gcgcagatcg aagcactcaa cgcggacccg
    28141 ggcgtgcacg gcatcctggt tcaattgccg ctgccgcggc agttcgaccc cgaggccgtg
    28201 ctggatgcag tgtcgcccga caaggacgta gacgggtttc acgcccacaa cgtgggccgg
    28261 ctggcgcagg gaacccccgc cttcgtgcct tgcacaccct acggcgtgat gaagatgctc
    28321 gaagccgagg cggtgccgat caagggggcc gaagcagtcg tgctggggcg gtccaacatc
    28381 gtcggaaaac cgatggcgcg gttgctgctc caggcaggcg caacggtcac cgtctgccat
    28441 tcacagacgc gcgatatcgc attccacacg cgccgggccg acatactggt cgtcgctgtc
    28501 ggacggccca gaatggtttc cggccagatg atcaagcctg gtgcggtggt gatcgacgtg
    28561 ggcatcaatc gtcttccggc cgagcagggc ggaaaattgt gcggcgatgt cgaattcgag
    28621 tcggcgcgtg ccgtggctgg gttgatcacg ccggtacctg gtggtgtggg gccgatgacg
    28681 atcacgatgc tgctcgccaa tacgcttgaa tctgccgagc gacacgcggc aaggttcgat
    28741 gtatggggcg ccgaatgaac gcgatgcatg tcgatccggt ggtgggaatc gtgatgggct
    28801 ccagctccga ctgggcgacg atgaaagcgg ctgctctggt cttcgaggaa ttcggcgtgc
    28861 cgcacgaggc gcgcgtggtg tcagcacatc ggacgccgga tctgctgttc cggtacgccg
    28921 aaacggccgc taatcggggc ttgaaggcaa tcatcgccgg cgccggtgga gcggcgcacc
    28981 tgccgggcat gctggccgcc aagacgacgg tgccggttct cggggttccc gtccagtcca
    29041 aggcgttgtc ggggctggat tctctgctgt cgatcgtgca gatgccgaag ggtgttccgg
    29101 tcgcgacgtt tgcgatcggc gagcccggcg cggcgaacgc tgcgctgttt gccgtggcga
    29161 tgctggcgcg cgaggatgag gggcttgcgg cgaagctcga tgactttcgc cgcaggcaga
    29221 cccaaaccgt actggacatg aaactttccg aacacgaatg atcctgccac ctgcaacgct
    29281 ggccatgctc gggggcggcc aattgggccg cttcttcgtt attgccgcac atgagatggg
    29341 ctatcgggtt gcggtgctcg acccggatcc gcacagtccg gcgggccgta ttgccgacga
    29401 acacctggtg gcggcctacg acgacaacga tgcgcttgag cggctcggca cgacctgcgc
    29461 tgcggtgacc actgaattcg agaatgttcc ggctgacacc ttgtcgttcc tgtcgaaata
    29521 tgtgccggtc cggccctctg cagaatgtgt cgcggtcgcg cagaaccgtg agcgggagaa
    29581 gtcgtttttc cgcgacggcg gatttccggt cgggccattt gccgttatcg gcagcgaggc
    29641 cgacatcgac gccgtgcccg acgatctgtt gccgggcgtg ctgaagatcg cacggttcgg
    29701 ctacgacggc aaagggcagg cgcgggtagc gacgcgcgcg gagatcaagg acgcgtttca
    29761 tgccctcaag agcgagcgct gcgtgctcga gcgcttcctg ccgctcgatc tggaggtttc
    29821 cgtcgtgctc gcacgcacgc aggacggcga ggcgaggtgc tttccggttg cagagaatcg
    29881 gcacaaaaac ggcatcctcg acgtgagcat cgttccggcg cgcgtcgatg accgcctgcg
    29941 ggcggaggcg gaggagcttg cccttggcgt tgcattgcat ctcgggtacg tcggtgtgct
    30001 cggcgtcgaa atgttcgtca gcgaggggcg acttctgctc aatgaaatcg cgcctcggcc
    30061 gcacaacagc gggcactaca ccatcgatgc ctgcgtgatc gaccagttcg agcagcaagt
    30121 gcgcgcgttg tgtggactcc gtctgggcga tgcccgactg catacgccgg ccgtcatggt
    30181 caatctcctc ggtgatgtct ggcacggcaa tgaaccgcac tgggggcgac tgttcgacga
    30241 cgccgggctg aagctgcacc tgtatggcaa gcatcatgcg cgtcccggaa gaaagatggg
    30301 ccacttcacc gtgctcgacg aagatgcggg gcgtgccgtc gatcgggctt atgcggcccg
    30361 cgagcgcatc gggattcgcg attgaccgtt gtcatgcaca gcgatgtcgt ccgggccgca
    30421 gaactcttgc gtgccggtga actcgtcgcg tttccaaccg aaacggtcta cggactgggc
    30481 gcggacgcgc tgaatccttc agctatcgcg aagatcttcg cggcgaaagg ccggccagcc
    30541 gatcatcccc tgatcgtcca tttgccgcct tctgcgcgcc tggacgactg ggcgcgagag
    30601 gttccgtccg gcgcacggcg cctggctgag gcgttctggc ctggcccctt gacgctgatc
    30661 ctgcagcgcc aatcgcaagt tcccgatcag gttaccggag gccaggatac agtcgccttg
    30721 cgtgttccgc gccacccggt tgcgctggac ttcctcgaag ccttcggcag cggcattgct
    30781 gcgccgtccg cgaaccgtta cgggcgcatc agcccgaccc tggcgcgaca cgtcgtcgaa
    30841 gaactcggca gcgcggtttc catggtgctg gacggcgggg cctgcgaagt cggcatcgaa
    30901 tcgaccatcg tcgatttatc gcgcggagag gcggtcctgc tccggccggg gttgatcggc
    30961 gccgaacgga tcgagagtgt tctcggacga cccctgtcgg tcgccgacgc gttggcgaag
    31021 ccggcgccac gggtgtcggg ctcgcatgcc gcgcactacg cgcctggaac gccgttgcga
    31081 ctcgtccgcc cggatcgcct gcgcgaagtg ctgaatgcac aacgacacgc cgggcatcga
    31141 tgtgcggtca ttgcctattc ccagcctgcc tttgccgagt cgccgcatgt ctggcgcatg
    31201 ctcgccgcgg atcctgcggg gtacgctcac gacctgtatt cggccctgcg cgaactcgat
    31261 tcgatggggt tgcgtgcgat cgtcgtcgaa cttccccccg aaggcgaagc ctggcgtgcg
    31321 attctcgatc gattgtttcg ctcggccgcc ggcagcggta tcgccgacga cgatcgtctc
    31381 tgacgcgcgg tgggctcggg cgtggcttcg ctgccaattg acatccaggt tgtatcgcac
    31441 gggcatgatg actgcctgcc gttgttgttg gacgatatcg aggttggacg atgcaacctg
    31501 tttgtcgccg agaatctggc ctcgtcgaaa ttgcagattt cgccacgcgc cgatctccat
    31561 tttgttcgca acgaaagcga ggtgggcttt gccgaaaacc ataaccggct ggcttcgctt
    31621 ggccatgctg aattcatcgc aatactgaat ccggacctgc gcatacccgc tggagtttgg
    31681 gaggggctgc tgccttactt cgacgacccc ggcgtagccg tggtggcgcc gcgggtctac
    31741 tccccccggg ggacggtcga ggacaatgcc cgtttcgtgg tgaccccgct acgcttcttg
    31801 aggcgcgtca tgcgctatcc cgacgtgacg ctcgattatc cggacattac ccggccgtgc
    31861 gaaccggact gggttgccgg gcttttcatg cttgtccgcc ggcgcgcatt cgaggatctg
    31921 ggcggattcg acgtcggctt ccggctctat tgcgaagatg tcgatttttg cattcgcgca
    31981 tggctggggg gctgcaagat actgtgtgtt ccgggtgaag gtgtgactca cgccgcgcgg
    32041 cgtgcgagtc ggagcaattt cacgcacttt ctgtggcacc tgtccagtat gttccggctc
    32101 tggcgatcgc cgggctggcg gcgtttcgtg ggttggaatc gtggcatttc cgaggtgtag
    32161 gcatcccgcg atttcttgcc ggttttggtc aaacttgtgc ggaacgggat ggagtctgac
    32221 atcttgtcga agtgggcgat caaacggacg cggcggcttc ggtcgatgat gaatcctgcg
    32281 agaagtcgat ccgctgccga aaaacgtcat ttcaatgact ttttgcggtt tcagcatatc
    32341 gattcggccc ggcagcgatc tctctgtctt caagagatcg ccatgcacac gaagttggcc
    32401 aggcagagag ccgatttccc gtgcggcaat tgttggacat gcgtgagcga tgcggctgcg
    32461 gagcaggtgg cgggctactt gcttctagat cgtcgatttg ggagcggcgc gctttgcttc
    32521 atcaagatac gagactgcag ttcgtttcat ttccttgacg aaatttcgtt cccttgctaa
    32581 cgttcgcgcc gcctgccgta aggccttcga gggcggcagt cgtttgtttg tttttcgaag
    32641 ggttgtgctg gagagttaag ggttgttctt aaacggtcgt ttggaaggtg ttcgggttct
    32701 ggtcagcgtc gtcatgttcg cggtcttggc ctttgtggca acaggttgtg cgacgacgaa
    32761 gccggaggac tcggacaagt ggtaccagtc tcagcggcaa gagttgagat ccaaggtcga
    32821 ggcgcggtgg gctgccttga tcaaagggga aaccgaaacc gcgtacatgt atctgtctcc
    32881 ggctcagcga tctgttgttt ctttgcaaca gtatcgccgc accttgggtg gggcagtaca
    32941 gtggcgggtg gcacaagtag atgacattcg gtatgattct cctactgtag cggtcgtctc
    33001 ggttgcgttg acctatcgtt tcgcttcctc ggcaacgggg aataaagaaa tcgagtcggt
    33061 tcgtcagatg caggagaagt ggctttacaa agatggggga tggtggtaca caacccagta
    33121 gattggagac catgttggtt tcccggtctt ctggtgaaag tgtcggccca ggcgcctggc
    33181 gtcgaatttt tttgaccttg caaggagttg gataaatatg aagctgctca aaaacacgcg
    33241 caagaaggtt gtgactctgg cggtcgcctc ggcgctcggc ggtgtcgcga tgatgtcggc
    33301 gcctgctcac gccatgaacg tggcccagga tggcaagggc gaagtgctgc tcttcccgta
    33361 ctacaccgtt ctgaacgggt tcgatacgat cctgtccgtc acgaatactt ctgatcgtac
    33421 gacggtcttc aagatccgct ggcgcgaagc caagaacagt cgcgaagtgc gcgacttcaa
    33481 cgtcgcgttg tcgccgcacg acgtctggac tgccggcgtg actgtcaacg gctcgggagc
    33541 tttggttcgt accttcgata agtcctgcac gtcgccgatt ttgccggacg gtccgaacgg
    33601 ttcgacccag atcgacttta cgaatgctgc ctatacgaat gccccgccgt tcgaggaaga
    33661 tggtgccggt actgcaataa gccgggttca ggaggggtac ttcgaaatca ttgagatggc
    33721 aatctcgacg gttccggaat cagcgattgg ttcgacaaac acgatcgaat tcgctacgca
    33781 acacgtcgct ggcgttccgc gcaattgcgc aacgttcgac gcggcgatga ttgatcccgc
    33841 aaaccttacc aatgccgcct ccggttcggg cgttttctca agcttcactg ctccggccaa
    33901 tgtgctcatc gggaatgcca ccctgatcaa cgtttcgaac ggcacggcat ttgacgccgc
    33961 gccgacggcc atccagaact tccaggatac cgacacgatc atcttcaggc cgcaggattt
    34021 gagcccgacc ttgggtgatg gtgagatcgt accgtcggcg ttccaactga tcgacggttt
    34081 gccaactacg acggcgatcg ccgctgttgc cgctccctca gtcgatgcaa ttagcgtact
    34141 gttgcaggcc aacaatgtga tcaatgagta cgccacaggg gaggctggcc aaagttcgac
    34201 caaaaccgac tgggttgtca cgttccccac caagcacttc tacacggacg atggcgtgac
    34261 tgcggctttg ccgccgttcc atgaagtgtt cgcgcctgct ggccggtctt gtgatcctat
    34321 cgcgctgtcg atctacgatc gcgaggaaaa gacgcaggtt acggttgggc aatcacagtt
    34381 ctcgccgcgc cccaatggcc ctggcggtgc gaccctgtgt aacgaagtca acgtgcttac
    34441 cttcaacaat tcggcattgc tgggtagcgg agtgcagttg agcgtcgaca caagcactgt
    34501 cggtacgtct ggttgggcaa accttagtct gaccggagct cctggtaata atggaatcac
    34561 cggcctacca gtgatcggct tctcggcgat cggtcgaaac aatgccgccg atgcaggaaa
    34621 caaccgcaac tacggttcgg gcatgccgca tgcccgcacc attgttccta cgccctaagg
    34681 ctgcatgact ttggtccgga gtggcgcaag tcattccggc tgtcattcaa caatggcccg
    34741 cccgcgggcc attgttttct tctggagaag ccccattctg cgggaggggc gcagtacgta
    34801 gggcatccaa ttgctcgcga tccgcaataa agtcacatca ggtcaggcgt gtcacaaatt
    34861 tttcctcgcg aggcctaaag ttggggagta gacttaacgt taacttctcg aagcaagttg
    34921 ttgtctagcc aaggctcgca ttttcaagga ggtgcattcc atgaccgttt cacaaagatt
    34981 tctacgcgcg gcagtaggtg gcgtgatcgc cggtacagtc gtcgtttccg tctgggcagc
    35041 caacgtttcg atcaatggca cgatgtattc gtgtacgggc ggtaccgttg ccggtgacgg
    35101 tagcatcagc tacacgggtt gtaatggcgg tacgagtacg agcggtacga gtacgagcgg
    35161 tacgagtacg agcggtacga gtacgagcgg tacgagtacg agcggtacga gtacgagcgg
    35221 tacgagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgg tacgagtacg
    35341 agcggtacga gtacgagcgg tacgagtacg agcggtacga gcggcgcagc caacgacccg
    35401 ggtatgttta ccggcacctg gataccggaa gggatgacag atgtttgggt tgttgaccag
    35461 tccgaaaccg gcggtgtggg cggtactaca gtgattcccg gttgtgccaa tcagaagtcc
    35521 caactcaatg tttgtggctc gtcgctggtg gaaacccgga acggagtgac gaccacaatc
    35581 actatggcgc aaggacacac catgtcgatc cgttacttga gcgaagcaac ggtcgtaaca
    35641 ggttcatatt tcaaagtttc gagtggcacg ggcgcgggtg tgcctgcggc ggttcaggtt
    35701 tcgctgagct cgcaacccgg cgattttacg ggcccgtcga cgtgcaacaa gacggccaat
    35761 tccggtggta accccctgat cattctcaac ggaaccaact gcccggttgc tccgaacacc
    35821 tactactacc tgaacattaa aacgacaacg gcatgttcac ctacgaactg cacgttcaaa
    35881 attgcggaac ctgcttcgtt caccaactaa cgaattcttt gtagggtaat tagcgcccgg
    35941 atgttactca tccgggcgtt ttgcgtttgc cggatagaac ttagtgcttt gtccacgagg
    36001 cgtaaccagt gttccgcaac ctttttttcg ccagacaatg aaactcctca gacccctcgt
    36061 gtttgtcgtt ggcacgaccg ttttttcaca aagtgccttt tctgctgact cgacgtttac
    36121 cgacggcgaa tcgtcgatct cgctttcaga actcaagact gagttgctgt cgctgccgcc
    36181 cgatatccgc gcaaagattt ccgccgacaa gaaagctctc gcgcgttttg cagggggcgt
    36241 gctgcaggat cgtcgcattg ccgatgcggc tgtggccgcc ggattcacca agacagacga
    36301 aatcaaggca aaaatacgga gggctgagag ggacgtggtc gtgcagagtt atctcactgc
    36361 ggaagcggct cgaacaacga gcgaggcgca ggactttcgc tcgcttgcac gcgagcagtt
    36421 cgaggtaaat cgcgcagcgt atgcagttcc cgaggcagtt cgcatttccc acatcttggt
    36481 ccgagtggac ccggaagacg atcgcttgca agaacgggag atgcgcgaaa aggccgaatc
    36541 gatactgtcg aaactgaagg agggcgccga cttttcagaa ttggccaaac agaactcgga
    36601 cgacaagggc tctgcgaatc gcggcgggga actccctggt tgggtcgaaa aagggaagac
    36661 cgttccgccg ttcgaaaagg ccgcgttcgc cctcaaaccc ggcgaactga gtgatctcgt
    36721 gcgtacccgg tacggtttcc acatcatcaa gcaactcgag tatcggcggg cagccaatca
    36781 agagttcggc gcggtcgagg cgcaactgac gaacaagctc cgaacggatt tcctgaaaca
    36841 gcgacagagc gaattcgtgg cccggtttgc cggcacccag gacatccaga ttgatgacgc
    36901 aaccttcgag gcggtgacaa agaactagtg tgagcaagtc tcgcggcgta tccttcaata
    36961 tgggtcgccg ctgcaaaggc tgacgctgta agttcacggg ccatgtggcg ctgtaacggt
    37021 tgttcgtgat gatctttttg caaagttcgg tcaaattggg ctcagtggca aaatggcagt
    37081 ttccgcccga gctagcggac accacaaccc tacataaatg ctgttcaact cagcggagta
    37141 tctgctcgca tttctcccga tctgctcgct tgtctacttc tggctgaatt ccaagcgact
    37201 ggctgtggct ggcaaagtct ggctcgtcgc ggcaagcctg ttcttctatg cgtggtgggc
    37261 gccggcatat ctcgtgctga tgctggcttc cgtcttggcc aatttctggt tgggcgtccg
    37321 tctgaatgca gacgcctccc cggatcgatc cgccggatcc cgattgctcg tcgtcggcgt
    37381 gatattcaat ctcggccttc tcgcctggtt caaatacgcg ggattcgtgg tcgagaacgc
    37441 caacctccta ttgggagttc agttcccggt gccgaatatc gtgctgcctc ttgcggtgag
    37501 cttctttacg tttacgcaaa tggcatatct cgtcgactgc cgtaggagaa ttgcggggga
    37561 atacagcttc tcgaactatg tcctcttcgt gacgttcttt ccgcacctga ttgccggccc
    37621 aatcctgcac cacagggaaa tgatgcccca gttcgagtcg cggaagaacc tccgtcctcg
    37681 accgcacaac cttgcactcg gcctgagcct gatcagcatg ggactgttca agaaggtcgt
    37741 gatcgccgat acgttcagcg tctgggcaac ggccggattc gatcagccga tcagtctgtc
    37801 cttcttcgag gcctggatct cgagcctttc ctatacgttt cagctctact tcgacttcag
    37861 cggctatacc gacatggcat tgggctcggc gctgctgttc aacatccgct tgccgatcaa
    37921 cttcaattcg ccctacaagg ctcggaacat ccaggagttc tggcggcgct ggcacattac
    37981 attgagccgc ttcctgcgcg attatctgta cgcccccctg ggcggaaacc ggcgaggtcg
    38041 cgcccgggtg ctggccaatc tgatgatcac cttcgttctc ggtgggctat ggcatggcgc
    38101 gagctggatg ttcgtcatct gggggggact gcatggcgct gcaatgatca ttcaccgagc
    38161 ctgggtgcag cttggcatcg cgctacccca atggcttgcg tggttgatca cgttcaactt
    38221 cgtcaacctt gcctgggtat tcttccgcgc ccgcgacatg gctgatgcga agcgcgttct
    38281 cgaaggcatg gcaggtatca acggcgtcgt gcttccaccc gcattcggcg actggttgcc
    38341 tcaactgaag tctttgggaa tcagcttcgg acccatcctg cgcacgggca acgacgcctt
    38401 tcaaatggtg ctctggatgg ttggcgcctt cgtgatcgtc ctcgcattcc gtaacagcaa
    38461 ccagctggcg ccatttaccg gcgaagcgcg cgcgatcggg ccgggaaggg ccatcctttg
    38521 tggcctcatg gcatcggcat cactggtcgg catactcgct tcgaaataca gcgagttcat
    38581 ctatttcaac ttctgacatg cgccgcaatt cgcttcgaca cggtgcacgt cggttcgtgc
    38641 aactctactt tctgttcctc gtcctgccac ttgcgacagg attcgtaccg ctcttggttt
    38701 atgaccctct ccagatctat cacaaaccct gggcgcgacc ccaaacgctg cacagcaact
    38761 ttcgtcttca ggcagccgca atcatcaggc gcgagccatt cgacggactg atactcggga
    38821 cgagcatgac agagaacacc tcggccgagg aagcaggcag gctgttcgac gaaagcatgg
    38881 cgaacctgtc gatcaccgcg tcggacttca tcgagcgttc cctcatcctg gacgacatct
    38941 tccgccgccg gcagatccgc cacgtcatct acacgctcga ccacgtctac atcggcaccc
    39001 ggaaggggta ttctctgtac ccgttgccaa ccttcgactt cctgtatgac cggaatccgc
    39061 tcaacgacat ccgggtctat ctcaacgaac acttcgcgaa gtgcctgctc cactggtcgg
    39121 gtgctccaga ctgcgtcggc acggccgtct cgctcgacag gcccaatgcg tggatcaatc
    39181 aaacggaata cgccgtgcgc ttcggcggga ttcgtaactg gtgcggcgcc aaagataatt
    39241 accagatccg cgacgcctac atgcaactgc gcaaggccat cgataacaag gcgagcgggg
    39301 ttgagcaatc tccatcggca gaggagctgc agcagcgagc cgaggccgca aagcgttatg
    39361 tggacgagaa tgtactccgg cacgtcaggc gccatcccga gacccggttc gatttcttct
    39421 ttccgcccta ttaccgcgcg acgtatggaa tctggcacca ggcacgtccg atccattccg
    39481 ctgttcacat cgcgatgatc cgatatcttg cggagcaggc cgaagccctg cccaacatgc
    39541 gcgtttccgg atttgaaacc gaatcgtttc cggacgacat cgcaaattac aaggatctct
    39601 cgcattacgc ggatgcgatc gattcgaaaa tccttgctgc gctgaaagcg gaatcgcacg
    39661 tcattacgcg ccgaaacgtt gacgcgtacg tcgacgcgtc ggtggcacgc gcaagggagt
    39721 tcgatctgga ggggctcgcc gcctcgcttc gcaattgcgg tgcaggcaac tgaaccggaa
    39781 cgcaatccat gaatccgcac gataaccttc ccgtgacgcc agctgcgatg gctcgcggcc
    39841 tgtggcgcaa ccgcgacctg attcgacaga tgacgtggcg agaagtcctc gggcgatatc
    39901 gtggttctct ctttggtctc gcatggtcgt tcttcaatcc ggtgttgatg ctcgttgtct
    39961 acacgttcgt cttctcgggc gtgttcaagg cgcgctggat ggtcgaaggt gatgtgcagg
    40021 ccagccatgc ccagttcgcg ctcgtactgt tcgtggggtt gatcctcaac ggactgtttg
    40081 cagagatact gaaccgggca ccgtcgctcg tactcggcaa cgtcaactac gtcaagaagg
    40141 tggttttccc gctcgagatc ttgccggtcg tcgcagccgg tgcagcggtc tttcatggcc
    40201 tcgtcagtac gggcgtcctt gttgcggcca ttctgctggt caatggaagc ctgccctgga
    40261 cagcagtatt cgcaccactt gtaatggttc cgctcgtcgt tctcgcgctc ggcttcgcat
    40321 ggtttcttgc gtcactgggc gtctatctgc gcgacgtcgg ccagacgata ggcatactga
    40381 cgaccgttct gatgttcctc gcacccgtct tctatcccac tgcagcgctt cccgagttca
    40441 tcagaccctg gctactgctg aaccccctta cactcgtcat cgagcaggca cgagacgtac
    40501 tgatctgggg gcggctgccg tcgtggcgtg gtctgcttgt ctactccggc gtggcggtgt
    40561 cgtgtgcgtg gttcggttac gtctggtttc agcgaacccg aaaggggttc gcagacgtac
    40621 tctaagatgt cctctgcgat cgcgaacgag atgagcggga acgaagcggc gagcgcgcgc
    40681 ggagcggaga tgtcccccgt cgtgatcagc gctcgcggca tctcgaagtg ttatccgatc
    40741 tacgacaagc cgcgagatcg attgaagcag tttctcctgc cccgtctgcg acgctatgcc
    40801 ggaaaagcgc cgggacaata tcaccgcgag ttctgggcgc ttcgcgacgt gtcattcgac
    40861 atctttcgtg gcgagaccgt cggcatcatc ggcaggaacg gcgcgggaaa atcgacgttg
    40921 ctgcagatca tttgcggcac cctcacgccg acgtccggcg tcgtcgagac gcgcggcagg
    40981 attgctgcgc tgctcgaact tggcgcgggg ttcaatcccg aattcacggg cgccgagaac
    41041 gtgttcctca atggtgctct gctgggattg agccgggagc aggtggaggc ccggttcgat
    41101 gccattgccg cattcgccga catcggcgaa tttctcgacc agcctgtcaa gacctattcg
    41161 agcggcatgt atgtgcggct tgccttcgcc gtacaggcat gtgtcgatcc cgagattctt
    41221 gtcgtggacg aggccctgtc cgtcggggat atcggttttc agtacaaatg tttccggcgc
    41281 atggaggaac tgcgcgatcg cggcgtgacc atcctcatgg tcacgcattc gacgggcagc
    41341 attctccagt acgccgatcg ctgcatcgtg ctcgacggcg gcagggtttc ccacgacacg
    41401 ccgcaggtgt tggaggcggt gcttgcctac gagaagggca tgatggcgca acagcacctg
    41461 cctgaatcga cggtgtctcg gccatcggtt gtatcggccg tgacgtcgca ggaactggcg
    41521 gcgaggcagg cgagccagcg caatgcggca ttggaagagg tgcggttcgg gtcggcgaga
    41581 gcgatcatcg atgagctcca cgtacactgt ggcgccagtt ctggcgaccc ggaccaaccg
    41641 gtcattcagt cgggtgacga ggtcgtcttc cgattccgga tactctcctc cgattggatt
    41701 ccggatgtcg tgctcggcgt atcgctttcg aagaccatgg gaggcgacat ctggggcgac
    41761 aacaacctga atgcaggcgt gcggatcgac ctgcccgcag ggccgacgga ggtcgactac
    41821 cgcgtcagac tgccggtcag cgcgggcgag tatctgattc actgcgggct ggcccgcttc
    41881 gacaaggatg ccagagagga actggatcaa cggcgcccca tgcgcaagct gacggtctgg
    41941 tcgccacgca gccaggtcgg cgttgtccat gcgcccgtga ctgtgaccgt tcggtcggga
    42001 gcgggcacgt gatcgcggtc tttctcgtgc cgttccccgt gcgggctgtt ccggcgccgt
    42061 atctctgggt gttctacaag atgctctcgg cctatcgcga gagagtcctg ttccttatcg
    42121 gagaggacta cattgccgac ccgcagcgct tcgctgcgga aggccgctgg gaggtcggtg
    42181 aacatgcctg caacgattac ggctatcgca taccgtcccg atcagacctg gagcagcatg
    42241 cgctgcgctt cctgccggca gacctctttc acggtctgct gcgcgaatgt ctggacaatc
    42301 ccctagaggc gtttcgcagg atgcttaccc ggcgcatccc gcagctcgag gcggcaatcg
    42361 accaggcgct gacggcagag atcgcgggtg gcgcgaagat cgaagcaatc gtgacctggt
    42421 gcaactgccc gtcactgacc gcgattgcga atcgccatga catcagggtt gttcacctcg
    42481 aggttggacc gctgcgtgca ccggagtatc gaccgaccgc ctatttcgat ttcagcggcg
    42541 tcaatggaca caccgaggcg gcggcccgat tcgccgcgat cggtggcgaa ccgccggcgt
    42601 tcgatctcgt tgccttgcga gggttcttca ccctgcaaag tgagtcaccg cagccggcta
    42661 tgggcggacg cgtcggggtg gcgttgcagg tcgaggacga ttccaatctt gtcgcgtatg
    42721 gcaacggctt cgacaaccag tcgctgatcg ttcatgcgaa gctgaagtat ggggggcggg
    42781 cgaagcttgt cgtacgcagc catccgggga gcgggtactg gctcaaaccg gggcagggat
    42841 tcgagctcga tcagtcgccg gacagcattg cgtttgtgcg cggttgcagc cggatattga
    42901 cgatcaactc cagcgtcggt ctcgagtcgg tgctttgggg cgtgccggtt acggtgctcg
    42961 gcgatacgtc gtatgcattc ctgctggagg cccgtgataa tcgcgcgctg ctcgcacgcc
    43021 ttgccttcta tctgtacggt tacctcgtgc cacgacaagc cctgttcgat ctcgcctacc
    43081 tgcgattccg gctgacgcag ccatcagagg cgcgaatcgt cgcttatcac ttgcagttcc
    43141 atgtgccgga ccacgatgcg gcgggggccg accttgaaga caatccatgg cgccttatcg
    43201 catcggtgct tggcgattcg ggaaggtaat gcccttggac tcatcaaggt ttcgccggcc
    43261 cgatgaccgc ttcacgcggc ctcctgaccg gatgatcacg agtctcgtga ccccgagtgc
    43321 gccccaatga cagacgggtc gcttcgtgtc ggcctcgccg tgcaacgggg cactcgaact
    43381 ggcgaaatgg ttgcgctgac ggggttccgc ggcctggctg ccctgtgggt gtttctctac
    43441 cacgcctggg gtcaatcggg gatggccttc gtcggtttca acctcggcga atggcaggtc
    43501 gacctgacgc ccttctttcg catgggaggc gccggggtct cgatattctt cgtcctgtcc
    43561 gggttccttc tcgcactgcc gtttgccgaa tggcaggccg ggcggcgcga caagcccgcg
    43621 acgtggctgt acctgcaaca ccgcgtgttg cgggttttcc ccgcatatta tgtccagctt
    43681 gcgattctgc tcgcgtacgc ataccacctt cacggagcgt cggcattgcc cacgccgacc
    43741 gacctgctgc agcatctatt catgctcttc gtaccgccgc cgctgggtgt cgagccgcgc
    43801 aacggtgtct ggtggacgct gccgatcgag ttttccttct atctggttct gccgctgctt
    43861 gcgccgttgc taagatggaa tcgactctgg ttgctggtgt cgctcagcct tgcatcgatg
    43921 gtgctgtggc gccatggtgt cgtcaccgtc ctggccggca gccccgttcc agtgcgggtg
    43981 attgcgtcgt accaattgcc gggcagcatg gacatgttcg gcatcggcat gctggctgcg
    44041 gtggttcttg tgaacagaga tgcgctgacg cgatggaccg acgtgttcgc caaccgggat
    44101 cgcttggtct ttctgggaat cgggctgctg actgtggcca tctactggct gagtcagcag
    44161 gcaagcctct actgggccga caatcccatc ttctatctct ggacgccagc gctgagtctt
    44221 gccgtcgcag gcatcctgct cgcgggggcg agcgggggaa ggttggccaa tgcgctgctt
    44281 ggcaaccggg tgatgcattt catgggaatc gtcagctaca gcatgtacct ctggcacttt
    44341 ccggtactcg tcgcactcaa ggaggcgggc ttattcaccg gcgagggcgt cgttcggctc
    44401 ggatcggcgc tcctcgtcgg tttgccgctg acgcttgcag tatcggcggt ttcctactat
    44461 ctggtcgagc gccccttcat gcgcatgcgg cggcgggaac gaggctgaac ggcagatgat
    44521 cgacgtgatc gttccggtct atcgtggcca tgaacaaacg cggcgatgcc tcgacagcgt
    44581 tctcgcgaat tgcgatgtcc gcgcagtcga ggtcgttgtt gtcgatgacg catcgccgga
    44641 acccgcactc gcggaatacc tcgacgcgtt gagtcgaagc ggctcgatca cgttgctgaa
    44701 gaacgaaacc aatcgcggtt tcgtcgaaag cgtgaatcgc gggatgtcac ttcaccgcga
    44761 tcgcgatgtc gtactcctga acagcgacac cgaagtggct gccggctggc tggagaggtt
    44821 ggccgcatgc gcgtatcgcg agccggatat cggcaccgtg acgccgttct cgaacaacgc
    44881 aacgatctgt tcctacccgt tcgatggatg ggccggtggc gtgccgggaa cgctcggcct
    44941 gtcccggctc gatcggctga tcgccacgac aaacgccggc aggcacatcg agcttcctac
    45001 cgcagtcggc ttctgcatgt acatccggcg agcgtctctc gatgccgtcg ggaatttcga
    45061 cgtcgaacgc ttcggtcgcg gctatggcga ggaaaacgat ttctcgttgc gcgcccgtgc
    45121 cgtgggatgg cgcagcgtac ttgccgcgga cgtattcgtc ttccatcagg gcagcgtcag
    45181 tttcggcacg gaacgcaccg ggttgcagcg cgccggcctg caacgcctgc tggaagcgca
    45241 tccccgatat ctcgacgctc tcggggactt ctcccgtcgc gatccggttg cctgcgtgcg
    45301 gcgtgccatt gacgacgccc gatacgcgct cggtggcgac gaacgggatt ccgtcatttg
    45361 cgagcagcgt acgagacggg gcgctacgcc cggagtgtcc ggtccggttc agctgcattt
    45421 cacgcacagc tgggatggcg gcaccaaccg ctggatcgcg gatttctgtg cgtacgatac
    45481 tgtccggcgc aatctggttc tgcgttcggt tagcaatcgc gattttgcag cgtggcgcat
    45541 agaactgtgc gatccgcagt tcctcgacgc ccccctgatg cagtgggatc tcgaaaagcc
    45601 gattcgggca acggatacgt tgaatctcga gtatcgcctc atcctggacg aaatcgttgc
    45661 gaccttcggc gtgcaggcca tggtcgtgtc ctcgctcgtc ggccactcgc tcgaggcgct
    45721 cgacaccgga ttgccgacgg ttgtcgtact tcacgacctg tatccgttct gtccggcgat
    45781 gttcgcgaca ttcggccagc cctgcaccac ctgcgactca acggcactcg cgcgatgcat
    45841 gcaaggcaat ccccacaacg cgttctggca caacaccgat gccggattct ggctggcctt
    45901 ccgcgaatcc tatgcaaggc gcctggcgca accgtggatc aggatcgcgg cgccgtcggc
    45961 aagcgcatgg ggccggtgga tgcagctatt ccccgggatc gcgggccggt cctgcgcgac
    46021 cattccgcac ggattgccgc cgttgccagg aatccgcgaa agcacaattt ccggggaacc
    46081 cgttgccggg caaaaactgc gactggtcgt tcctggccgc ttgctgccgc acaaggggct
    46141 ggcgttgttt gccgcggcac tgccgcgatt ggtgcagcat gcggacatcc tgcttctcgg
    46201 ttgcggccgg ttcggcgcgc cgttccatga tgctgaaggc gtgacggtcg tgagtcacta
    46261 tgcgcacgaa aatctcgtcc gacatatccg tgaattcatg cccgatgccg cgctgctgct
    46321 atcggtcgtc cccgagtcct tcagctacac gctgagcgaa atgctcgaaa tcggcttgcc
    46381 tgtgatcgca acccgcgtcg gtgccttcgc ggagcggatt cgcgatggcc gagatggcct
    46441 gctcgtcgcg ccggatcccg atgcggtcgt tgccgcggta gagcggctcg ggcgcgacgg
    46501 cgatctgctt gccgcgctac aacgagccgt tctgtcaagg ccggcgcgca ccgcgtcgca
    46561 aatggtcgcc gattatcatg cgctgcttcc gctggcccgg agtgccggtg cggacgatac
    46621 gcaatgtgca ttgcaggcct cgattgccct gaccttgtcg atccgcgcag caaaggagag
    46681 aaagcgcctg gaggacgccg gccatcgttt gcgagcatca ctcatcgaag cacaggacca
    46741 tgggcgcgaa ctcggtgggc aactcgccgc ggcgcaccgt cgctgcggcg aggccgaagg
    46801 tcgtgcgagc cgtgcagtgg cggatggcga agcgatgcgc cgttcccact catggcagct
    46861 cacggcaccg ctgcgaaagg tagccagctt gctgcgcagt ttggtcggat cggcgtcacc
    46921 ccgcgccggt gacgggctgc atgccgagga agatgcgagc cccgtgaacc aaaccggcgc
    46981 gccccggatc gtgttgtccg acgatgccga agtccgtgtc gcggcacgcc acaagctgcg
    47041 cgaacagttg gcgattcccg gctgctcgag gatcgtcctg ggctggtcgt cgggcgcaga
    47101 cagggcgttc gccgcgcact tcctcgacct ggcggcgggc tttaacgcgg cgcgcaacga
    47161 cgtgttcttc ttactctgtg gcgtagcggg gggagatccg gccatggcgg atcggcaaat
    47221 cgggttgatg gtcgcaacac gcaggctgtt tgtcgagcca ttcgaagagt cggtggccga
    47281 caccctgctt ggcgccgatg ttgtccttct ggacgcgagc gatccgcttc ggagtatcct
    47341 gactgccgag tcgtcgcgcg gcggaccgaa agcattggtt attgcgccgc cgagcgtcgg
    47401 cagaacgggg ggcgagaccg cgatgccgaa cgatgtttca cacgttgcgc ctgtcgacgc
    47461 ggcacgtctg ctcagtacgc tgctcgatca gtccggcccg gagaaggcgt gaagaaccgt
    47521 cgcaagccgc tcgtgtcgcc ggtgccgaaa tcggccgctg tcgaccaatc tgctgtcggt
    47581 ggttgcctga gacccgtctc ggcaacgcat ccgctgattg cgctcagcgg gttggcggct
    47641 ttttttgcgg ggatcgtggt gctctccctg cttcggccct tccagatggt ggcctatgat
    47701 gcggcgttgc tgatactcgt catcgcattg ggtgtcttcg tgccggacat gctgtggcgg
    47761 cgcgtgtggc gcaacccatc ggccggattg atgcgtagtc cggccgaccc ttcgtgggac
    47821 cgcacgatca ccaagtttgc cggattcgcc ggcagcctcg gtttcattgc ctttctctac
    47881 tggctgtttc cggagtactc gaccaggtcg ccgttctacc agcatttctg gacactgctg
    47941 cgtgtcgcga ttccggcggt gctcgtcatc gcactgccct acatctacct tgtcgatggc
    48001 cgcatgcaaa atccgcacga cgggttgtgg cagttcgggc gcgtcctcac cttccagtgg
    48061 tcggatgtcg atggccgcac ggtggggcag cacctgctcg gctggctgat caagggcttc
    48121 ttcctgccgc tgatgttcgg c
//
LOCUS       DAOQNN010000003        34478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_327_length_34478_cov_11.046539, whole genome shotgun sequence.
ACCESSION   DAOQNN010000003 DAOQNN010000000
VERSION     DAOQNN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 34478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34478
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_327_length_34478_cov_11.046539"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PKV97_00590"
     CDS             complement(<1..585)
                     /locus_tag="PKV97_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNB04399.1"
                     /translation="MKAQTLYEKLWSSHVVHEEADGTALIYIDRHLVHEVTSPQAFEG
                     LKLAGRKPWRIGSIVATADHNTPTDHWDLGIQDPVSRQQVETLDANIREVGSLAYFPF
                     KDARQGIVHVIGPENGATLPGMTVVCGDSHTSTHGAFGCLAHGIGTSEVEHVLATQCL
                     LQKKSRTLLIRVDGQLGRGVTAKDVVLAIIGRIGT"
     gene            complement(662..1477)
                     /locus_tag="PKV97_00595"
     CDS             complement(662..1477)
                     /locus_tag="PKV97_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB04400.1"
                     /translation="MQSIFKIALPVLLAGTIATACQTVQTTHGGAVGVQRSQLMMVSA
                     QEVEQASNQQYQQMVAEARSKNALDRDPATVQRVRRIVSRLAAQTGAFRTDAPSWSWE
                     VHVFSSSELNAWCMAGGKMAIYTGLIERLALTDDEIAAVMGHEIAHALREHARERVSK
                     SMATGLGISVAGALLGVGQLGQDLMGQVAKVTFELPNSREHETEADRMGVELAARAGY
                     DPRAAVTLWNKMAGQSAGAPPQWLSTHPSHATRQRDLAEYAARVIPLYEGARR"
     gene            complement(1485..2654)
                     /locus_tag="PKV97_00600"
     CDS             complement(1485..2654)
                     /locus_tag="PKV97_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB04401.1"
                     /translation="MIPYWRLSAYYFFYFAFVGAFSPYFTLYLQSIALSATDIALLMS
                     LMQLMRVLAPNLWGWLAERLGMRIAIVRLSALASLAGFSVFFLTTEFAGLFAAMALMA
                     FFWSAVLPLIEGLAFAHLGEASHRYGRIRVWGSVGFIVAVLALGHSLDRLPIEAVLWI
                     TMSILVGIVLCSFIVPEAPRPPLQRDAASFGDTLRRPEVRALLGACFLMSAAHGALYV
                     FYSIHLVGIGYDKGVVGWMWTLGVLAEIGVFMWMPRISVRFSLRAILLFSFACAVARF
                     LMIGWGAHSLALLLLAQVLHGATFGAYHAAAIAVVNEWFPGRLQSRGQALYGSISFGA
                     GGMLGGLLSGYTWEGIGPAWTYTIGAGFALAGLLWLLLGWKGEPRPAHALHEPRR"
     gene            complement(2742..3872)
                     /gene="aroC"
                     /locus_tag="PKV97_00605"
     CDS             complement(2742..3872)
                     /gene="aroC"
                     /locus_tag="PKV97_00605"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNB04402.1"
                     /translation="MSGNTLGTLFTVTSFGESHGPAIGCIVDGCPPGLAICEADIQAE
                     LDRRKPGTSRHVTQRREPDTVEILSGVFEGVTTGTPISLLIRNQDQRSKDYGNIADTF
                     RPGHADYAYLQKYGLRDHRGGGRSSARETAVRVAAGAIAKKWLKERHGIVIRACMGAL
                     GPIEIPFVSWDEVDGNPFFAPNAAIVPELEAYMDALRKSGDSIGARIDVVASGVPVGW
                     GEPVYGRLDADIAYAMMGINAVKGVEIGAGFKSVAQRGTEHGDEMTPAGFLSNHAGGV
                     LGGISTGQDILASIAIKPTSSIRLERRSIDRAGNPVMVATEGRHDPCVGIRATPIAES
                     MLALVLIDHALRHRAQCGDVRTDTPRIPALAPAGSQRLPSPR"
     gene            complement(3968..4285)
                     /gene="ilvN"
                     /locus_tag="PKV97_00610"
     CDS             complement(3968..4285)
                     /gene="ilvN"
                     /locus_tag="PKV97_00610"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNB04403.1"
                     /translation="MMIEQVSDPLPQAGYAKVVLELDVNNHPGVMSHICNLFARRAFN
                     VEGILCMPVSDGKRSRIWLLVFEDQRLEQMVRQLEKLEDVLNVRRHGAEHEVFERLEG
                     FFH"
     gene            complement(4285..5952)
                     /gene="ilvB"
                     /locus_tag="PKV97_00615"
     CDS             complement(4285..5952)
                     /gene="ilvB"
                     /locus_tag="PKV97_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HNB04404.1"
                     /translation="MMQMTGAQLMVRLLERQGVRTVAGIPGGAILPFYDALSGSEHIH
                     HVLARHEQGAGFIAQGMARVSGVPQVCIASSGPGATNLVTAIADACLDSIPMVVITGQ
                     VPQAMIGTDAFQEVDIYGITVPITKHNFLVRSAQELLEVIPDAFRIAMSGRPGPVLID
                     VPKDVQNQLVTVAEFPAPAVADPAPQLDLAAVELAAKMINEAEQPVLYLGGGVVASGA
                     APLAVQLAEQAGLPTTMTLMALGAMPMDHPLSIGMLGMHGARYTNFVLEEADVLVCVG
                     ARFDDRAIGRAAQFCPNAKIVHIDIDRSELHKIKNAHVAIHANVKCALEALLPRVQAT
                     LRKRWLSHVDSLKTRFPMQFPGQDDPRTHYGLIQAVAAALDDRAIVATDVGQHQMWVA
                     QAYPFRRPRQWLTSGGLGTMGFGLPSALGAALAEPGRTVVCFTGDGSFKMNIQELATL
                     AEEGLNVKIVLMNNNALGLVYQQQTLFYGQRLFASKYRTEPDFVKIAEGFGVPAVDLD
                     LADNPRAALAEALHRPGPCLIHATIDREQFVYPMVPPGAANTEMIGG"
     assembly_gap    6209..6308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6319..7980)
                     /locus_tag="PKV97_00620"
     CDS             complement(6319..7980)
                     /locus_tag="PKV97_00620"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HNB04405.1"
                     /translation="MEKIWLKSYPPGIPAEIDVDEFRSLGDLFERSVARYADKTAYHC
                     MGKSITYAELDALSARFGAWLQGALKLPRGARVALMMPNVLQYPIALFGTLRAGYTVV
                     NVNPLYTARELEHQLKDSGAEVIVILENFAHTLEHVRAQVPIRHIVVTSLGEMLGLVK
                     GAIVNFVVRKVRKLVPPWRLDGHVRFSTALQRGAAHELQPAQVGHEDVAFLQYTGGTT
                     GVAKGAMLTHRNVIANLQQAHAWIAPYVGEGKEIIITALPLYHIFSLTANCLTFLKIG
                     AKNVLIPNPRDIPGFIKELGQHRFTAITGVNTLFNALLNNPEFAKLDFSTLRVSLGGG
                     MAVQQVVAEKWKKVTGVSLVEAYGLTETSPAVTINPLDLAEFNHSIGLPVSSTEISIR
                     DDAGHEVPLGQRGELCVRGPQVTPGYWQRPEETARIFTHDGFLRTGDIATVDERGFVR
                     LVDRKKDMILVSGFNVYPNEVEDVVAAHPGVLEVAAIGVPDERSGEAVKVFVVRKDPE
                     LTAEALIAFCRENLTGYKVPHHVEFRDELPKSNVGKILRRELRPK"
     gene            complement(8194..8643)
                     /locus_tag="PKV97_00625"
     CDS             complement(8194..8643)
                     /locus_tag="PKV97_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB04406.1"
                     /translation="MLVSEILAIKGKVLFTIGPNRPIAEAVEIMNEQDVGSLVVFSRG
                     EMVGMLTFRQVLQAVQQGGADWQSLQVEAVMLREPRVASPDMEMDELRRLMVEHHQRY
                     LPVMDGNTLLGVVSFHDVAKAVLEEQSFENRMLKNYIRNWPAGEGEE"
     gene            complement(8736..9059)
                     /locus_tag="PKV97_00630"
     CDS             complement(8736..9059)
                     /locus_tag="PKV97_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNB04407.1"
                     /translation="MTYVVTERCIRCKYTDCVDVCPVDCFREGPNFLVIDPEECIDCT
                     LCVAECPVEAIYSEDDVPDDQKGYIALNAELAKQWKPIVERKDPLPDADHWARIKGKL
                     GELKR"
     gene            9434..9760
                     /gene="trxA"
                     /locus_tag="PKV97_00635"
     CDS             9434..9760
                     /gene="trxA"
                     /locus_tag="PKV97_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HNB04408.1"
                     /translation="MSEHIHHVTDGNFDDEVLQSQTPVLVDYWAEWCGPCKMIAPILD
                     DVAKDYAGKLKVAKLNIDDNQETPAKYGIRGIPTLMLFKGGNVEATKVGALSKSQLTA
                     FIDSNI"
     gene            9939..11198
                     /gene="rho"
                     /locus_tag="PKV97_00640"
     CDS             9939..11198
                     /gene="rho"
                     /locus_tag="PKV97_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNB04409.1"
                     /translation="MSELKALHVSQLLQMAMDAGVEGANRLRKQELVFALLRNRARRG
                     EPICGDGALEVLPDGFGFLRSPDTSYLAGTDDIYVSPSQIRRFNLRTGDTIEGEIRTP
                     KDGERYFALTKLDKINGRPPEECKTKILFENLTPLHPQECLKLEREVRGEENVTSRII
                     DMIAPIGKGQRGLLVAPPKSGKTVMLQHIAHAITANHPDVKLIVLLIDERPEEVTEML
                     RSVKGEVVASTFDEPATRHVQVAEMVIEKAKRLTEHKYDVVILLDSLTRLARAYNTVV
                     PASGKVLTGGVDANALQKPKRFFGAARNIEEGGSLTIIATTLVDTGSRMDDVIYEEFK
                     GTGNMELHLDRRMAEKRVYPAINVNRSGTRREELLLKADVLQKVWILRKLLYGMDDID
                     AMEFLLDKVKATKSNAEFFDAMRTGRG"
     gene            complement(11280..12131)
                     /locus_tag="PKV97_00645"
     CDS             complement(11280..12131)
                     /locus_tag="PKV97_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNB04410.1"
                     /translation="MSIGKIGVVGAGTMGNGITQAFAVAGRKVVMMDVAEAQVQRGLA
                     TISASLDRLIKKDRMSAPQKDAAMACITTVSSLDALADCDLVIEAATENVDLKLRIFA
                     DLDRIVKPEAILASNTSSISITRLAAGTQRPGQVIGMHFFNPVPVMALVELINGLQTS
                     AATYSAVEDVAKAIGKTPIKVKNSPGFVVNRMLCPMINEAVFALGEGLATAAEIDEAM
                     KLGCNHPIGPLALCDLIGLDVELAVMQVLYEGTKDPKYRPAPLLVEMLEAGYLGRKSG
                     KGFYDYK"
     gene            12433..13146
                     /locus_tag="PKV97_00650"
     CDS             12433..13146
                     /locus_tag="PKV97_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNB04411.1"
                     /translation="MLHTSPPYAVRQETEVAEPKTLVEAAYRSLRRNIIEGRLSPGEK
                     LRIEHIKNDYGVGGATLREALSLLTSDALVVSQGQRGFRVAPVSLEDLADITETRVML
                     ECSALRQSITLGDEAWEAELTAAFHRLTRAEEKLAEGNNREQWEERNRIFHEVLIAAC
                     PSRWTKHFLSILYHQAERYRHLSLRRSDGTRRLHDEHAALFEAAMGRDAETATRLLGE
                     HIRLTLQSVSLDKAATASH"
     gene            complement(13350..13925)
                     /locus_tag="PKV97_00655"
     CDS             complement(13350..13925)
                     /locus_tag="PKV97_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNB04412.1"
                     /translation="MSNKSLDIIHDEHRALAAMLSGLRGLVASIEAGRLKPDFELMAS
                     MIEYIEMVPEKVHHPKEDSYLFARLRERSAEALPIIERLEAEHREGDARMATLRAALE
                     AYRREGESGLAGFQGALKTYIEHEWQHMNTEEHLIFPLARKHLSAEDWAHIDAAFLAN
                     DNPWQGPAGKYAALFTRIVNTAPAPVGLGGG"
     gene            complement(13946..14659)
                     /locus_tag="PKV97_00660"
     CDS             complement(13946..14659)
                     /locus_tag="PKV97_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB04413.1"
                     /translation="MSNMLLEAKEIHTYYGASHILHGIDFNIREGEGIALMGRNGMGK
                     STLIKSMLGIVRPRHGRVLVRGDDYTRARTFQTAQQGIAYVPEGRGIFKSLSVRENLL
                     MSARAGVDGRREWTFDRVMETFPRLGERINNGGWQLSGGEQQMLTIGRALMTNPDLLI
                     LDEATEGLAPLIAMEIWRIVKEIRKTGIATVIVDKNHGAVTSICDRAMILVKGQVVFD
                     GTSDEIRAQPELIQKHLGV"
     gene            complement(14652..15428)
                     /locus_tag="PKV97_00665"
     CDS             complement(14652..15428)
                     /locus_tag="PKV97_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB04414.1"
                     /translation="MSVTTRNEVLLQADKLTRRFGGLVANQDISVTLERQMIHVLLGP
                     NGAGKSTCINMLSGDLPPSEGRIHYLGRDITALTAAQRSQVGIGRSYQRTNIFPQFTV
                     LENVRLAAQSRRQQPWRLFSKAMEQKWALEKARACIEEAGLGKRVDWVSGVLSHGEQR
                     QLEIAMVLATDAQVLLLDEPLAGMGSEESASMVEVLKRLRQTRGILLVEHDMDAVFAV
                     ADMITVMVNGQLLECGPPAQIKASVAVQQAYLGTEDSFHV"
     gene            complement(15425..16390)
                     /locus_tag="PKV97_00670"
     CDS             complement(15425..16390)
                     /locus_tag="PKV97_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB04415.1"
                     /translation="MNANASLIPKLVALAIVACVPLSGESFYIEMVGKVLVMAIFAMS
                     LDLLVGFTGLVSFGHAAYFGIAAYAVAILSPEYEAASLWYVLPASVGLSALAAFVIGL
                     FVLRTKGIYFIMVTLAFAQMAYFIFHDTPLGGGSDGKYLNNKPSASIFGWEPFDLTNE
                     VHMFYFVLAVLVLVYLFLARVLQSPFGRVLVGIKSNEHRMQSLGYYTFRYKLGAFTLA
                     GALGGLAGFLYAVLSGFVTPELLSWHESGNVLLMVILGGMGNLAGAIAGAFAFEAMRE
                     IFADITKYWQLLMGGVIVAVVLFLPGGLAGVPGRIKRALQGGDAK"
     gene            complement(16395..17261)
                     /locus_tag="PKV97_00675"
     CDS             complement(16395..17261)
                     /locus_tag="PKV97_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB04416.1"
                     /translation="MDFASFLIQTLNSLQYGLLLFLVASGLTLVFGIMGIINLAHGSF
                     YMIGAYLAFVLTSATGSLVLAVVLGIPLALAFGAFLEWALFSHLYKRDHLEQVLLTYG
                     LILIFEQLRSLLVGDDVHGVAIPAFLDASIQLTEVMSYPVYRLAISAICIVLAVALYY
                     LIQRTRLGMMIRAGNDDRGMVEALGININMIYRVVFALGVALAALAGMLAAPISSVFP
                     NMGSHVLIISFVIVVIGGIGSVWGALVAALIVGFADTFGKVLVPELSGIVVYVVMAVV
                     LLWRPEGILKKG"
     gene            complement(17368..18540)
                     /locus_tag="PKV97_00680"
     CDS             complement(17368..18540)
                     /locus_tag="PKV97_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB04417.1"
                     /translation="MKSTFKLAGNLALTLALAAAGIGAANAADKVKVGLMLPYTGTYA
                     ALGTAITNGFKQFVDENGGKLGGREVEYFTVDDESNPAKATENAKKLVSRDKVDVLVG
                     TVHSGVALAMAKVARDTKTLMIVPNAGANELTGPLCSPYIFRSSFSAWQPSYAMGEAL
                     AKKGIKNVATVTWKYSFGEESVAGFKEAFEKGGGKLVKEMTLPFPNVEFQPFLTEIAA
                     AKPDAVFVFFAGAGAAKFVADYAAAGLKNSIPLYGPGFLTDGNLGAMGGAGEGLLTTL
                     HYADGLTHAKDAAFRTKYATTYKLQPDVYAVQGYDAAQMYEAGLKAAGGDPEKQEEII
                     KGMESAKIDSPRGAFTLSKAHNPVQDIYMRQVKGDQNVMLEVVSKGLEDPARGCKM"
     gene            complement(18696..19484)
                     /gene="dmpH"
                     /locus_tag="PKV97_00685"
     CDS             complement(18696..19484)
                     /gene="dmpH"
                     /locus_tag="PKV97_00685"
                     /EC_number="4.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047437 - 4-oxalocrotonate decarboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-3-hexenedioate decarboxylase"
                     /protein_id="HNB04418.1"
                     /translation="MKLNQQTIEQLAEHLENAELQAFEVVKITEAHPDMDWDDAYAIQ
                     AEILRRKLARGNRVVGLKAGLTSRGKMKQMGVDTPVFGWLVDYFSVPDGGEIKTSELI
                     HPKVEPEIVFVTKRALKGPGCHIGAVLAATDFVMPGIEIIDSRYKDFKFDLKSVIADN
                     CSSTRFVLGGQAAPVAGLDLRTLGVVLEKNGEPVAVGAGAAVLGHPAAAIAALANHLG
                     ARGEEIPAGTMILSGGVTEAVSVQAGDSVNLRIQSLGSVSTRFI"
     gene            complement(19514..20296)
                     /gene="dmpE"
                     /locus_tag="PKV97_00690"
     CDS             complement(19514..20296)
                     /gene="dmpE"
                     /locus_tag="PKV97_00690"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxopent-4-enoate hydratase"
                     /protein_id="HNB04419.1"
                     /translation="MDQKTIEALGDSLYDALVARTPIAPLSAAHPDMTIEDAYHVQQR
                     MIARRLEQGDRVIGKKIGVTSKAVMNMLGVHQPDFGYLLDSMVFNEGESVDMDTLIQP
                     KAEGEIAFLLKKDLQGPGVTAADVLAATEGVMACFEIVDSRIQDWKIKIQDTVADNAS
                     CGVFVLGDQLVDIADLDLALAGMVLEKNGEIVVTGAGAATMAHPVNAMVWLANMLGSL
                     GIALKAGDIVLSGAMGAMVPVARGDNLRMTIGGIGGCSVRFV"
     gene            complement(20311..21771)
                     /locus_tag="PKV97_00695"
     CDS             complement(20311..21771)
                     /locus_tag="PKV97_00695"
                     /EC_number="1.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconic semialdehyde dehydrogenase"
                     /protein_id="HNB04420.1"
                     /translation="MIADKILNFIDGEYVATDKWYENRNPINNKVIGMVAEAGEKEVD
                     AAVKAAKAALKGPWGSMSLQKRIELLEALVVEINNRFDDFLEAECADTGKPKSMASHV
                     DIPRGAANFKVFADMVKNVPTEFFEMTTPDGGKAINYGYRRPVGVVGVICPWNLPLLL
                     MTWKVGPALACGNTVVVKPSEDTPRTAALLGEVMNKVGIPKGVYNVVNGFGANSAGAF
                     LTAHPDVDALTFTGETRTGEVIMKAAANGSRPVSLEMGGKNAAIVFADCDFDKAIEGT
                     LRSVFLNCGQVCLGTERVYVERPIFDKFVAALKAGAEGMKIGVPDDPAANFGPLVSKK
                     HQEKVLSYYKVAVEEGATVVTGGGVPQMPGELADGCWVQPTIWTGLPETARVIKEEIF
                     GPCCHIAPFDTEEEVLEKANDNKYGLACAIWTQDVSRAHRVAQKMEVGISWVNSWFLR
                     DLRTPFGGSKQSGIGREGGVHSLEFYTDLKNVCIKL"
     gene            complement(21917..22846)
                     /locus_tag="PKV97_00700"
     CDS             complement(21917..22846)
                     /locus_tag="PKV97_00700"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HNB04421.1"
                     /translation="MATTGVMRPGHIQLRVLDLDESVAFYKDVLGLVETGRDAAGRVY
                     FKAWDERDHNSVILRQADRAGVDLIGFKVKDKATLEQLDKDLQAYGVATERIPAGELL
                     ETGERVRFTIPTGHVMELYAEKTDVGNGQPYTNPDPWIAASEHGIAPHRFDHCLLYGP
                     NLEENLKLFTEVLGFHLVERVLLEDGKSLLATFISCSHKAHDLAFVAHPEPGKLHHLS
                     FLLDSWEKVLRAADIMSMNRVSIDIGPTRHGITRGSTIYAFDPSGNRFETFCGGYETY
                     PDHAPITWTFDEVGAGIFYHDRKLNERFLTVVT"
     gene            complement(22944..23303)
                     /locus_tag="PKV97_00705"
     CDS             complement(22944..23303)
                     /locus_tag="PKV97_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNB04422.1"
                     /translation="MNAPAPKVFHQVTIVETGEVYRCREDETLLAGMERLGKRGIPVG
                     CRGGGCGVCKVQVEAGEYSKRVMSRAYVSAEEEAAGIVLACRVKPLSDLRLAVLGKMK
                     KNVCATPSAAAADARQS"
     gene            complement(23305..26847)
                     /locus_tag="PKV97_00710"
     CDS             complement(23305..26847)
                     /locus_tag="PKV97_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNB04423.1"
                     /translation="MSSGKQISNTFTQEEGDIHLGGIDAIVRLTLDQVRTDARRGLKT
                     GMFVSGYRGSPVGMLDAALIKQQKLLVQNHIHFVDGINEDLAATAVWGTQMLHTVGKQ
                     KFDGVTGMWYGKAPGVDRSGDALKHANYTGIAKNGGVLAIAGDDPSCKSSSLCSQSEP
                     MLFHVGIPSLYPGNVQEILDYGLHGYQMSRLAGVWMGMKIVTNVADGTGTASVSPERL
                     NFVTPDLEFDGKLFTPNMNLGMNVRVEALEMEQSLYTRRLEVVKRYARANNLNNVVFP
                     NPDAWIGILTAGKTYNDLNQAFLEMGLDDAALRRYGIRILKMGMLFPMEPSIVREFAQ
                     GLEEIFVIEEKRPFLEMFAKQVLYGMANAPRIVGKFDEEEKELLPHYGEFESDVIVRA
                     LLKRLSRKTRIESAENWLKRLDEIHSRNKLPTAVRTAWFCSGCPHNSSTQAPDGSIVS
                     AGIGCHTMAMWMGRNVVMGTHMGAEGTQWIGMAPFTEAQHIFQNMGDGTYAHSGSLAI
                     RYAASTGVNITFKLLRNAHTSMTGGQAIQGEVPVTNMVSDLLANGVKKIIVTTDDPSR
                     FNGVQLPGGTEVWHRDRIEEAQRELAATPGCTVLLHDQECAAELRRARSRGKADEPVE
                     VTVINERVCEGCGDCGEKSNCMSVEPVQTEFGRKTRIHQSSCNKDFSCVKGFCPSFLT
                     ITPHPAPAAEGAPKKKKGRIPALERELPPPVKKVDDSLGFGIHVMGIGGTGSVTVVAT
                     LARAARLEGKHVIGLDQTGLAQKGGAVISDIKITHAPFKGSNKISDGRADLYLGFDIL
                     NATDPKNLDKCNPERTIAIVSTTQTPTGQMVTNRKALFPATSGLTGGIDRVTRKEHNV
                     FLDGEALAAGLFGDAMATNNFMVGVAFQAGTIPLQAESIEDAIRQSGVAVDMSLAAFR
                     WGRMAVVDRAFVEAEVARQKTGGQVVVLNKAPQLSPAARAIVDSIGASGEVKRLVEIR
                     VPELIAFQDEAYAKRYAEVIKRVVAGEQKAVSSSALAEAAARYLYKLMAYKDEFEVAR
                     LHTDPAFLAELDAQFPHGYSVKYNLAPPLLSKTDPVTGHPQKKQYGEWMFKAFKRLSG
                     LKRFRGSALDVFGKTEERQTERKLIEEYVQLLDQILARLNPVNHAAAVALASVPDEIR
                     GFGHVKEKNLVAARELQAARLKAFNEAQQERQVA"
     gene            27121..28455
                     /gene="benA"
                     /locus_tag="PKV97_00715"
     CDS             27121..28455
                     /gene="benA"
                     /locus_tag="PKV97_00715"
                     /EC_number="1.14.12.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase large subunit"
                     /protein_id="HNB04424.1"
                     /translation="MITTEYLDSLIEKDKEKGLFRCKREMFTNRELFDLEMEHIFEGN
                     WIYLAHESQLPKINDYYTTKIGRQPVFITRNKDNQLNCFINACAHRGATLARFKHGNK
                     ATYTCPFHGWTFNNSGKLLKIKDPSETGYPESFNCEGSHDLKKIARFESYRGFLFGSL
                     KADVQPLEDFLGESKKIIDMVVDQSPEGLEVLRGSSTYVYEGNWKLQAENGADGYHVT
                     ATHWNYAATQAQRKSRDAGDDIKAMSAGGWAKKGGGSYSFENGHLLLWTRWDNPEDRP
                     LMEQRDRLVKEFGEAKADWMIGNSRNLCLYPNVYLMDQFSSQIRVFRPLDVNRTEVTI
                     YCIAPKGESAEARARRIRQYEDFFNASGMATPDDLEEFRACQEGFMGRALEWNDMSRG
                     ATHWVEGPDEEADKIDLKPILSGVKTEDEGLYVAQHTYWLEEIRKAAAAKNA"
     gene            28471..28959
                     /gene="benB"
                     /locus_tag="PKV97_00720"
     CDS             28471..28959
                     /gene="benB"
                     /locus_tag="PKV97_00720"
                     /EC_number="1.14.12.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase small subunit"
                     /protein_id="HNB04425.1"
                     /translation="MSLSYENLCAFLYREARHLDDREWDEWLSMYDENCTFWMPAWDD
                     DGELTEDPQTEISLIWYGRRAGLEDRVFRIRTERSSATIPDTRTNHHISNVEVVEQND
                     KTVKLRFNWHTMSVRYNHTDHFCGTSFYTLDVSGASPLIKEKKVVLKTDYIRHVIDVY
                     HV"
     gene            29028..30038
                     /gene="benC"
                     /locus_tag="PKV97_00725"
     CDS             29028..30038
                     /gene="benC"
                     /locus_tag="PKV97_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase electron transfer
                     component BenC"
                     /protein_id="HNB04426.1"
                     /translation="MTFKIALNFEDGVTRFIDCNATETVADAAYRQGVNVPIDCRDGA
                     CGACKCKAESGKYELGFYIDDAMSEEEAKLGYVLTCQMHPKSDCVVNVPASSEVCKIK
                     HATLTATVAEVRQLSPSTLSLVLQGEGIGKLAFLPGQYANLTVPGTEQTRAYSFSSMP
                     ADSKVSFLIRNVPNGLMSGYLTGAAKPGDTIKLAGPIGSFYLRDVARPVLMLAGGTGL
                     APFLAMLDKLVAAGGSAHPVHLIYGVNTDADVVELERLDAFKAALPNFTYDVCVVADD
                     SSWPKKGYVTAHIEPAHLNEGNVDIYLCGPPPMVEAVAHDLRERKVEPNSFHYEKFAA
                     SV"
     gene            30066..30842
                     /locus_tag="PKV97_00730"
     CDS             30066..30842
                     /locus_tag="PKV97_00730"
                     /EC_number="1.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate
                     dehydrogenase"
                     /protein_id="HNB04427.1"
                     /translation="MANRFKNKVAVITGAAQGIGKRVAEKMAKEKGTLVLVDRSELVH
                     EVAKELSGKVEVLSLTADLEKFADCKRVMEAAVEKFGRIDILINNVGGTIWTKPFEHY
                     EEDEIEAEVRRSLFPTLWCCRAALPYMQEQGKGAIVNVSSIATRGVNRVPYGAAKGGV
                     NALTACLAFENAERGIRVNATAPGATEAPPRLIPRNTKEQSEQEKLWYKQIYTQSIDS
                     SLMKRYGTLDEQADPILFLASDDAAYITGTIVPVGGGDLG"
     gene            31149..32120
                     /locus_tag="PKV97_00735"
     CDS             31149..32120
                     /locus_tag="PKV97_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB04428.1"
                     /translation="MPIRFLNGQSNVFAAANPFEVSEYVRANVGSHSLRLPRASDASA
                     SLTHRRAGTLDLCRLSYGAQARVLSESLGDIYHAQFILQGYCSYTLANRTLDLPAGHV
                     LVLNPDEPVDLTYSDNCEKFIVRIPSAMLDDACTEHRWFKPNERIKFSPEPQRFEDID
                     SLLLLLRLLCEEAESELATPQMLQHYCRVVTTKLMVMLKHNVSMVAPTRHAPSFERLV
                     NYIERNIKLDLSAEDLAHYAGLSLRSLYLLFEKNVKTTPKNFVRQKKLEKVHSILSDP
                     GQACPNVTAVALEYGFSHLGRFSELYKSTYGVLPSQSIRCRQPQPGR"
     gene            32227..32625
                     /locus_tag="PKV97_00740"
     CDS             32227..32625
                     /locus_tag="PKV97_00740"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNB04429.1"
                     /translation="MERIAINPPELFSGKAFSQTFSISTPQRLIFVSGQVDCDREGKV
                     RNPDDLEAQLKGTLDNLEIALRAQGASMRDLVQVTIYVVGLRPEDTTRIREIRSAYFD
                     AERPPAVTLLGIERLAFPGLRVEIDIIAAQ"
     gene            complement(32697..33263)
                     /locus_tag="PKV97_00745"
     CDS             complement(32697..33263)
                     /locus_tag="PKV97_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNB04430.1"
                     /translation="MNAPAATPARITRIARRRARPGHEAEYEVMLREMLAKMREHKGF
                     LGGDLIPPEAAGEEYQLVIRFASEAELQAWDMSEARHELLERMKAVAEGEPEFRKLSG
                     LEAWFEPAVVPATMHPPRVRMAVVTWLGIFPTVSFFLWFVLPWIQPLPFLPRTAVLTA
                     LIVVTMTWVVMPRLTRLMRGFLNPPRKG"
     gene            complement(33488..34414)
                     /locus_tag="PKV97_00750"
     CDS             complement(33488..34414)
                     /locus_tag="PKV97_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNB04431.1"
                     /translation="MNISKIAAAVALGTLAIGGAHAKEGGDQYPNGAETWLAGAVPPP
                     GNYFINYFGYYGGDLVDGNGKKAPTGEVDAWFNAFRFVHTSEHKILGGNWGWHVIVPV
                     VHQEVDLGGSKSVSGLGDVTVNPFIVSWHEKNWHWAIGLDVNLPVGRYKEGDARQSIG
                     ANYWSFEPLFAFTYMGDTGWEVSAKVMYNIKTENSDFRSVPGGAKMDYQSGDEFHFDY
                     LVGKRFGPWGVGLSGYYLKQTTDDEINGQTIAANPGFWSEGRKGEVFAIGPTVSYTTK
                     GGVHFTGQWNRETGAENRFEGDKFILKLIMPL"
BASE COUNT         5786 a        11540 c        11347 g         5705 t
ORIGIN      
        1 ggtgccgatc ctgccgatga tggcgagcac cacgtccttg gcggtgacgc cgcggccgag
       61 ctggccgtcg acgcggatca gcagggtcct ggacttcttc tgcagcaggc attgggtggc
      121 gagcacgtgc tcgacctcgg aggtgccgat gccgtgcgca aggcagccga aggcgccgtg
      181 cgtggaggtg tgggagtcgc cgcagacgac ggtcatgccc ggcagcgtgg cgccgttctc
      241 cggcccgatc acgtgcacga tgccctggcg cgcgtccttg aacgggaaat aggcgagcga
      301 gccgacctcg cggatgttgg cgtccagcgt ctcgacctgc tggcgcgaca ccgggtcctg
      361 gatgcccagg tcccagtggt cggtgggcgt gttgtggtcg gcggtggcga cgatggagcc
      421 gatgcgccag ggcttgcgcc cggcgagctt gaggccttcg aaggcctgcg ggctggtcac
      481 ttcgtgcacc aggtggcgat cgatgtagat cagcgccgtg ccgtcggcct cctcgtggac
      541 cacgtggctg gaccagagct tttcatacag cgtttgagct ttcatcccgg agtgttccgc
      601 gtctgaaaaa ggaatcgatt atttcacaaa gcctgcaaaa ctgtgtaggc acgccccgcc
      661 ctcagcgccg cgcgccctcg tagagcggga tcacgcgtgc ggcgtactct gccaggtcgc
      721 gctgccgcgt cgcatgcgag gggtgggtgg acagccactg cggcggcgcg ccggccgact
      781 gtccggccat cttgttccac agcgtcaccg cggcacgcgg atcgtagccg gcgcgtgcgg
      841 cgagttccac gcccatgcgg tccgcctcgg tctcgtgctc gcgcgagttg ggcagctcga
      901 aggtcacctt ggcaacctgg cccatgaggt cctggccgag ctggccgacg ccgagcaggg
      961 cgcccgcgac cgagatgccc agcccggtcg ccatcgactt cgacacccgc tcgcgcgcgt
     1021 gctcacgcag cgcatgcgcg atctcgtggc ccatcaccgc ggcgatctcg tcgtcggtga
     1081 gcgcgaggcg ctcgatcagc cccgtgtaga tcgccatctt gccgccggcc atgcaccagg
     1141 cgttgagctc gctcgagctg aacacatgca cctcccagga ccacgacggg gcatcggtgc
     1201 ggaaggcgcc ggtctgcgcg gcgagccgcg agacgatgcg gcgcacgcgc tgcacggtgg
     1261 cggggtcgcg atcgagcgcg ttcttgctcc gcgcctcggc gaccatctgc tggtactgct
     1321 ggttcgaggc ctgttcgacc tcctgcgccg agaccatcat cagctgcgag cgctgcacgc
     1381 cgaccgcgcc gccgtgcgtg gtctgcacgg tctggcaggc ggtggcgatg gtgccggcga
     1441 gcagcaccgg cagggcgatc ttgaagatgg attgcatggg tcgattacct cctcggttcg
     1501 tggagtgcgt gcgcggggcg gggttcccct ttccagccca gcagcaacca gagcaggccg
     1561 gcgagcgcga agccggcgcc gatcgtatat gtccacgccg gcccgatgcc ctcccaggtg
     1621 taaccgctca gcaagccgcc cagcatgccg ccggccccga aggagatgct gccgtagagc
     1681 gcctggccgc gcgactgcag gcggcccgga aaccactcgt tgaccaccgc gatcgccgcg
     1741 gcgtggtagg cgccgaaggt ggcgccgtgc agaacctggg cgagcagcag cagcgccagg
     1801 ctgtgcgcgc cccagccgat catcaggaag cgcgccaccg cgcaggcgaa ggagaacagc
     1861 aggatcgcac gcagcgaaaa gcgcaccgag atgcgcggca tccacatgaa gaccccgatc
     1921 tcggcgagca cccccagcgt ccacatccag cccaccacgc ccttgtcgta accgatgccg
     1981 accaggtgga tcgaatagaa cacgtagagc gcgccgtgtg ccgccgacat caggaagcag
     2041 gcgccgagca gggcacgcac ctcgggacgg cgcagggtgt cgccgaagct cgcggcgtcg
     2101 cgctgcagcg gcgggcgcgg cgcctcgggg acgatgaagc tgcacagcac gatgccgacg
     2161 aggatggaca tggtgatcca cagcaccgcc tcgatcggca ggcggtcgag cgaatgcccg
     2221 agcgcgagca cggcgacgat gaagccgacc gagccccaca cgcggatgcg gccatagcgg
     2281 tgcgaagcct cgccgaggtg ggcgaaggcg agaccctcga tcagcggcag caccgcgctc
     2341 cagaagaagg ccatcagcgc catcgcggcg aacagcccgg cgaactcggt ggtcaggaag
     2401 aacaccgaga agcccgccag gctggcgagc gccgacagac gcacgatcgc gatacgcatc
     2461 cccaggcgtt ccgccagcca gccccacagg ttgggagcca acacgcgcat cagctgcatc
     2521 agcgacatca gcagcgcgat gtcggtggcc gacagcgcga tcgactgcag gtagagcgtg
     2581 aagtagggcg agaacgcgcc gacgaaggcg aagtagaaga agtagtacgc cgacaggcgc
     2641 cagtagggaa tcatcgcgcc cactccgggt acgcgcgctc accccctcca gcccctgcgc
     2701 ccgggcggcg ccccgcaccg ccccgccctg cccgcatcgc ctcagcgcgg cgaaggcagg
     2761 cgctggctcc ctgcgggcgc gagcgcgggg atgcgcgggg tgtcggtgcg cacgtcgccg
     2821 cactgcgcgc gatggcgcag cgcgtggtcg atcagcacca gtgcgagcat ggattcggcg
     2881 atcggcgtcg cgcggatgcc cacgcagggg tcgtggcgac cctcggtggc gaccatgacg
     2941 ggattgcctg cgcggtcgat cgagcgacgc tcgaggcgga tgctcgaggt cggcttgatc
     3001 gcgatgctgg cgagaatgtc ctgtccggtg gagatgccac cgagcacgcc gcccgcatga
     3061 ttggacagga agcccgcggg cgtcatctcg tcgccgtgct cggtgccgcg ctgcgcgacc
     3121 gacttgaacc cggcgccgat ctccacgccc ttgaccgcgt tgatgcccat catcgcatag
     3181 gcgatgtcgg cgtccaggcg gccatacacg ggctcgcccc agccgaccgg gacgccgctg
     3241 gcgaccacgt cgatgcgcgc gccgatcgag tcgcccgact tgcgcagcgc gtccatgtag
     3301 gcctcgagct cgggcacgat cgcggcgttg ggcgcgaaga aggggttgcc gtcgacctcg
     3361 tcccaggaca cgaagggaat ctcgatcggg ccgagcgcac ccatgcaggc gcggatgacg
     3421 atgccgtggc gctccttcag ccacttcttc gcgatcgcgc cggccgccac ccgcaccgcg
     3481 gtctcgcgcg ccgacgagcg cccgccgcca cgatggtcgc gcaggccgta cttctgcagg
     3541 taggcgtagt cggcgtggcc ggggcggaag gtgtcggcga tgttgccgta gtccttgctg
     3601 cgctggtcct ggttgcggat cagcagcgag atcggggtgc cggtggtgac gccctcgaac
     3661 acgccggaga ggatctcgac ggtgtcgggc tcgcggcgct gggtgacgtg gcgcgaggtg
     3721 cccggcttgc ggcggtcgag ctcggcctgg atgtctgcct cgcagatcgc cagtcccggc
     3781 gggcagccat cgacgatgca gccgatcgcc gggccgtggg attcgccgaa ggaggtgacg
     3841 gtgaagagcg tgccgagggt gttgccggac atggatttcc tgcctgatcg cgatgacgga
     3901 acggggacgg gtcgcgagtc tagcacgcgt gccgtcccct tctgcttggg cgccggagcg
     3961 ccgcccctca gtggaagaag ccctccaggc gctcgaagac ctcgtgctcg gcgccatgcc
     4021 ggcgcacatt gagcacgtcc tcgagcttct cgagctggcg caccatctgc tcgaggcgct
     4081 ggtcctcgaa caccagcagc cagatgcgcg agcgcttgcc gtcggacacc ggcatgcaca
     4141 ggatgccctc gacgttgaac gcgcgacgcg cgaagaggtt gcagatgtgg ctcatcaccc
     4201 cggggtggtt gttcacatcc agttccagaa cgaccttggc atagccggct tgcggcagcg
     4261 ggtcggaaac ctgttcgatc atcatcagcc tccgatcatc tcggtgttgg cggcacccgg
     4321 cggcaccatg gggtagacga attgttcgcg gtcgatcgtc gcgtggatca ggcacggacc
     4381 ggggcggtgc agcgcctcgg cgagcgcggc gcgcggattg tcggcgagat cgagatccac
     4441 cgccggcacg ccgaagccct cggcgatctt cacgaagtcg ggctcggtgc ggtacttcga
     4501 agcgaacagg cgctgaccgt agaacagcgt ctgctgctgg tacaccaggc cgagcgcgtt
     4561 gttgttcatc agcacgatct tgacgttcag accctcctcg gccagcgtcg caagctcctg
     4621 gatgttcatc ttgaagctgc cgtcgccggt gaagcagacc acggtgcgcc cgggctcggc
     4681 gagcgcggcg ccgagcgcgc tcggcaggcc gaagcccatc gtgcccaggc cgcccgaggt
     4741 gagccactgg cgcggccggc ggaagggata ggcctgcgcc acccacatct gatgctggcc
     4801 gacgtcggtg gcgacgatgg cgcggtcgtc gagcgcggcg gcgaccgcct ggatgaggcc
     4861 gtagtgggtg cgcgggtcgt cctggccggg gaactgcatc gggaagcgcg tcttcaggct
     4921 gtcgacatgc gacagccagc gcttgcgcaa ggtcgcctgc acgcgcggca gcagcgcctc
     4981 gagcgcgcac ttgacgttgg cgtggatggc gacgtgggcg ttcttgatct tgtgcagctc
     5041 ggagcggtcg atgtcgatgt ggacgatctt cgcgttcggg cagaactgcg cggcgcggcc
     5101 gatcgcgcgg tcgtcgaagc gtgcgcccac gcacaccagc acatcggcct cctcgagcac
     5161 gaagttggtg tagcgcgcgc cgtgcatgcc gagcatgccg atcgacagcg ggtggtccat
     5221 cggcatcgcg ccgagcgcca tcagcgtcat cgtggtgggc aggccggcct gctcggccag
     5281 ctgcaccgcc agcggcgcag cgccggaggc gaccaccccg ccgccgaggt agagcaccgg
     5341 ctgctcggcc tcgttgatca tcttcgccgc cagctccacc gccgccaggt cgagctgcgg
     5401 ggccggatcg gcgaccgccg gggcgggaaa ctccgcgacc gtgacgagct ggttctgcac
     5461 gtccttgggc acgtcgatca gcaccgggcc ggggcggccg gacatggcga tgcggaaggc
     5521 gtcggggatc acctcgagca gctcctgcgc cgagcgcacg aggaagttgt gcttggtgat
     5581 cggcaccgtg atgccataga tgtcgacctc ctggaaggcg tcggtgccga tcatcgcctg
     5641 cggcacctgg ccggtgatga cgaccatcgg gatggagtcg aggcaggcgt cggcgatcgc
     5701 ggtgaccagg ttggtcgcgc ccggaccgct cgaggcgatg cacacctgcg gcacgcccga
     5761 gacgcgcgcc atgccctggg cgatgaagcc cgcgccctgc tcgtggcggg cgagcacgtg
     5821 gtggatgtgc tcgctgcccg agagcgcatc gtagaagggc aggatcgcgc cgccgggaat
     5881 gccggcgacg gtgcgcacgc cctggcgctc gagaaggcgc accatcagtt gcgcgccggt
     5941 catttgcatc atgttgctct ccttggcttc gggttcgacg gccggaccgg cgggcacaaa
     6001 agcaagaccc ccgccgggct tgcgcgccga cgggggtctg gtattcctga ttctcgagtg
     6061 tccggtcccg ttacgtcgcg cgcatgccta cgcctactac gcgtactaca acgaccagcg
     6121 acaggagcga gggacggccg gaaacggaca ttggaacgga tctgcaagaa agcgtaaaca
     6181 acgtgacggc gagtttaatc acgaaaagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnncc gcgcgcgctc acttcggccg cagctcgcgg cgcaggatct tgccgacgtt
     6361 gctcttgggc agttcgtcgc ggaactcgac gtggtgcggc accttgtagc cggtgaggtt
     6421 ctcgcggcag aaggcgatca gggcctcggc cgtcagctcc ggatccttgc gcaccacgaa
     6481 gaccttcacc gcctcgccgc tgcgctcgtc gggtacgccg atcgcggcga cctcgagcac
     6541 gcccggatga gcggcgacca cgtcctcgac ctcgttcggg tacacgttga agccggacac
     6601 caggatcatg tccttcttgc gatcgaccag gcgcacgaag ccgcgctcgt cgacggtggc
     6661 gatgtcgccc gtgcgcagga aaccgtcgtg cgtgaagatg cgcgcggtct cctcggggcg
     6721 ctgccagtag cccggcgtga cctgcggacc gcgcacgcag agctcgccgc gctggccgag
     6781 cggcacctca tgcccggcat cgtcgcggat gctgatctcg gtggaggaca ccggcaggcc
     6841 gatcgaatgg ttgaactcgg cgaggtcgag cgggttgatc gtcaccgccg gcgaggtctc
     6901 ggtgaggccg taggcctcga ccagagacac cccggtgacc ttcttccact tctcggcgac
     6961 cacctgctgc accgccatgc cgccgcccag gctcacccgc agcgtggaga agtccagctt
     7021 ggcgaactcg gggttgttga gcagtgcgtt gaacagcgtg ttgaccccgg tgatcgcggt
     7081 gaagcggtgc tggccgagct ccttgatgaa gcccgggatg tcgcgcgggt tggggatcag
     7141 cacgttcttg gcgccgatct tcaggaaggt gaggcagttc gcggtgagcg agaagatgtg
     7201 gtagagcggc agcgcggtga tgatgatttc cttgccctcg cccacgtagg gggcgatcca
     7261 cgcatgcgcc tgctgcaggt tggcgatgac gttgcgatga gtgagcatgg cccccttggc
     7321 cactccggtc gtgccgccgg tgtattgcag gaaggcgaca tcctcatggc ccacctgcgc
     7381 cggctgcagc tcgtgggcag cgccgcgctg caaggccgtg gaaaagcgca cgtggccgtc
     7441 gagccgccac ggcggcacca gcttgcgcac cttgcgtacg acgaagttca cgatcgcgcc
     7501 cttgaccagg ccgagcatct cgcccaggct ggtgaccacg atgtggcgta tcggcacctg
     7561 cgcccgcaca tgctcgagcg tgtgggcgaa gttctccagg atcacgatga cctcggcgcc
     7621 ggagtccttc aactggtgct cgagctcgcg cgcggtgtag agcggattga cgttgaccac
     7681 cgtgtagccc gcgcgcagcg tgccgaagag cgcgatcggg tactgcagca cgttgggcat
     7741 catcagcgcg acgcgcgcac cgcggggcag cttcagcgcg ccctgcagcc aggcgccgaa
     7801 gcgcgcactg agcgcgtcga gctccgcgta ggtgatcgac ttgcccatgc agtgataggc
     7861 ggtcttgtcc gcatagcgcg ccacgctgcg ctcgaacagg tcacccaggg aacggaattc
     7921 gtcgacgtcg atttccgccg gaatgcccgg ggggtagctc ttcagccaga ttttttccat
     7981 ggtctcctcc ttttgctgga aataccgccg gagcgccgcg atggcgcccc ggctccgccc
     8041 tcccaaggcg tcggacgggc aagcggccct tccgttcatt ccaaccatgc cgcacccgat
     8101 cttggtgtgg gtgtcgaccg aacgggcgca gcccgttcct ggaggtgcgg cggttcgacc
     8161 gccgctgccg ggcgcggggc ccggcgatgc tgcttattcc tcgccctcgc ccgcgggcca
     8221 gttgcggatg tagttcttga gcatgcggtt ctcgaagctc tgctcctcga gcaccgcctt
     8281 ggcgacgtcg tggaaactca ccacaccgag cagcgtgttg ccgtccatca ccggcaggta
     8341 gcgctggtgg tgctcgacca tcagccggcg cagctcgtcc atctccatgt cgggcgaggc
     8401 cacgcgcggc tcacgcagca tgaccgcctc gacctgcagc gactgccagt ccgccccgcc
     8461 ctgctgcacc gcctgcaaca cctgccggaa ggtgagcatg ccgaccatct cgccacgcga
     8521 gaacacgacc agcgagccga cgtcctgctc gttcatgatc tcgaccgcct cggcgatggg
     8581 tcggtttggg ccgatggtga ataacacctt gcccttgatc gcgagaatct cgctcaccag
     8641 catgtcggtc tccgtgccgt tgcacttaca ggtttgatga cgctcaccat cttagtgcat
     8701 cgccgcgtgc gcgcaaagcc agccgcggcg tgccttcagc gcttcagctc gccgagcttg
     8761 cccttgatgc gggcccagtg gtcggcatcc ggcagaggat ccttgcgctc gacgatcggc
     8821 ttccattgct tggccagttc ggcgttgagc gcgatgtaac ccttctggtc gtccggcacg
     8881 tcgtcctcgg aatagatcgc ctcgaccgga cactcggcca cgcacaaggt gcagtcgatg
     8941 cactcctccg gatcgatgac aaggaagttg ggcccctcgc ggaaacagtc gaccggacag
     9001 acgtccacgc agtcggtgta tttgcagcgt atgcagcgct cggtaacgac gtaggtcatc
     9061 tcgactctcc aggcaggatc ggccgccgcc cgctgcggct ggccgaaggg gggacaatat
     9121 agaccagccc ttggcttttt tgccacggca agcaggcgct tgcccgtccg ccagcagcga
     9181 tttccttgac ggcgaccccg gcatcgcata agtttcggaa aaatttcttt acggaaggcc
     9241 tctccttccg ctggatcgtc gtgcatcgca gcagcgcctc ccgctcgcgg cgagatcgca
     9301 gggggtccgc cccttcccgc acgcagcatc cgttcgcatt accccgcaac accccatacc
     9361 ccgcagcacc cgttacccgg caacgagggc ttccgccttc gtcaccaccc cctgccagcc
     9421 ttagtgagga atcatgagcg agcacatcca ccatgtgacc gacggcaact tcgacgatga
     9481 agtgctgcag tcccagaccc ctgtcctggt cgattactgg gccgagtggt gcggcccctg
     9541 caagatgatc gcgccgatcc tggacgacgt cgccaaggac tacgcgggca agctcaaggt
     9601 cgccaagctc aacatcgacg acaaccagga gacgcccgcg aagtacggca tccgcggcat
     9661 cccgacgctg atgctgttca agggcggcaa tgtcgaggcg accaaagtgg gcgccctttc
     9721 caaatctcaa ctgaccgcct tcattgacag caacatctaa gccggaccag gctccggccc
     9781 gttcccgcgg ttcctcgcgc gcccgtcgcc gcggcgcgcg caaccgggac ggcaattccc
     9841 cccagaatcc gcagaccgaa gacgatctgt tcgacgtcga gaacggcggc gaggaaggtt
     9901 cttccacgcc gcgcgacccc gacgcgcccg cgctccacct gtccgaactc aaggccctgc
     9961 acgtcagcca gctgctgcag atggcgatgg acgccggtgt cgagggcgcg aacaggctgc
    10021 gcaagcagga actcgtcttc gcgctgctgc gcaaccgtgc ccgcaggggc gaaccgatct
    10081 gtggcgacgg cgcactcgaa gtgctgcctg acggcttcgg cttcctgcgc tctcccgaca
    10141 cctcgtatct cgcggggacg gacgacatct acgtgtcgcc ctcgcagatc cggcgcttca
    10201 acctgcgcac cggcgacacc atcgagggcg agatccgcac gcccaaggac ggcgagcgct
    10261 acttcgcgtt gaccaagctc gacaagatca acgggcggcc gccggaagag tgcaagacca
    10321 agatcctctt cgagaacctc acgcccctgc atccgcagga gtgcctgaag ctcgagcgcg
    10381 aggtccgtgg cgaggagaac gtcaccagcc gcatcatcga catgatcgcg ccgatcggca
    10441 agggccagcg cggcctgctc gtcgccccgc ccaagagcgg caagacggtc atgctgcagc
    10501 acatcgcgca cgcgatcacg gccaaccatc ccgacgtcaa gctgatcgtg ctgctgatcg
    10561 acgagcgccc ggaggaagtg accgagatgc tgcgttcggt gaagggcgag gtcgtggcct
    10621 ccaccttcga cgagcccgcc acccgtcacg tgcaggtcgc cgagatggtg atcgagaagg
    10681 ccaagcgcct gaccgagcac aagtacgacg tcgtgatcct gctcgactcg ctgacccgtc
    10741 tggcgcgcgc ctacaacacc gtggtgccgg cctccggcaa ggtgctcacc ggtggtgtcg
    10801 acgccaacgc gctgcagaag cccaagcgct tcttcggtgc ggcgcgcaac atcgaggaag
    10861 gcggctcgct gaccatcatc gccaccacgc tggtcgacac cggcagccgc atggacgacg
    10921 tgatctacga ggaattcaag ggcaccggca acatggagct ccacctcgac cgccgcatgg
    10981 cggagaagcg cgtgtatccg gcgatcaacg tcaaccgctc cggcacccgc cgcgaggagc
    11041 tcctgctcaa ggccgacgtg ctgcagaagg tgtggatcct gcgcaagctg ctgtacggca
    11101 tggacgacat cgacgcgatg gaattcctcc tcgacaaggt caaggccacc aagagcaacg
    11161 ccgagttctt cgacgcgatg cgcaccggcc gcggctgatt ccgcgctcga cagcaaaacg
    11221 ccaccttgga ggtggcgttt tttcttgtgc ggccgcgccg cgccgtgcgc ggccgacgct
    11281 cacttgtagt cgtagaagcc cttgccgctc ttgcgaccga ggtagcccgc ctcgagcatc
    11341 tcgaccagca gcggcgccgg gcggtacttg gggtccttgg tgccttcata gaggacctgc
    11401 atgaccgcga gctcgacgtc caggccgatg aggtcgcaca gcgcgagcgg gccgatgggg
    11461 tggttgcagc cgagcttcat cgcctcgtcg atctcggctg cggtcgccag gccctcgccg
    11521 agcgcgaaca ccgcctcgtt gatcatcggg cacagcatgc ggttgaccac gaagcccgga
    11581 ctgttcttga ccttgatcgg cgtcttgccg atcgccttcg cgacgtcctc gaccgcggag
    11641 taggtcgccg cggaggtctg caggccgttg atcagctcga cgagcgccat caccggcacc
    11701 gggttgaaga agtgcatgcc gatcacctgg ccgggacgct gggtgccggc ggccaggcgg
    11761 gtgatggaga tcgacgaggt gttgctggcg agaatcgcct ccggcttcac gatacggtcg
    11821 aggtcggcga agatgcgcag cttgagatcg acattctcgg tcgccgcctc gatcaccagg
    11881 tcgcagtcgg cgagcgcatc gagcgagctc acggtggtga tgcaggccat cgcggcgtcc
    11941 ttctgcggcg cgctcatcct gtccttcttg atgaggcggt cgaggctggc cgagatggtc
    12001 gcgagcccgc gctgcacctg cgcctcggcg acgtccatca tcaccacctt gcgccccgcg
    12061 accgcgaacg cctgggtgat gccgttaccc atcgtgcccg cacccaccac tccgatcttg
    12121 ccgatactca tcgccttcgt tctccgcaca ggattttggt cgacgcaacg gccgccggac
    12181 gacggtctcc ataccgctgc gtatgcgcgg ggactttagc ggattccgcg ggcgggtgcc
    12241 agcgtcgcgc ggccgcacag cgggaggatc gaaaggcgct ccacccaagc gtttctcgat
    12301 gctcggcagc tcgggcgaag cgctgcaagc aagccggttt ttcgatcatt tctcgatatt
    12361 tttcttgtga gtcccccggg gcagcggcta cattagagag gcctccatcc gtcccccttc
    12421 ctctccaaga tcatgctcca cacctcgcct ccctacgccg tccgccaaga aaccgaggtc
    12481 gccgaaccga agacgctggt cgaggcggcc tatcgcagcc tgcgccgcaa catcatcgag
    12541 ggccgcctgt cgccgggcga gaagctgcgc atcgagcaca tcaagaacga ctacggcgtg
    12601 gggggcgcga cgctgcgcga ggccctctcg ctgctcacct cggatgcgct ggtggtgtcg
    12661 cagggccagc gcggcttccg cgtcgccccg gtctcgctcg aggacctcgc cgatatcacc
    12721 gagacgcggg tgatgctcga gtgcagcgcg ttgcgccagt cgatcacgct cggcgacgag
    12781 gcctgggagg ccgagctcac cgccgccttc caccgcctca cgcgcgcgga ggagaagctc
    12841 gccgaaggca acaatcgcga gcagtgggaa gagcgcaacc gcatcttcca cgaggtgctg
    12901 atcgccgcct gcccctcgcg ctggaccaag cacttccttt ccatcctcta tcaccaggcc
    12961 gagcgctacc gccacctctc gctgcgtcgc agcgacggta cgcgccggct gcacgacgag
    13021 cacgccgccc tgttcgaggc cgcgatgggg cgtgacgccg agactgccac ccgcctgctc
    13081 ggcgaacaca tccgcctgac gctgcagtcg gtcagcctcg acaaggccgc caccgccagc
    13141 cactgagccg gccacccggg cttgccgccg cacagcggcg aaaagtcaaa aaaatggccc
    13201 gccgaagcgg gccaaacccg aaatcacctc tgcgcgggcg cgcagaaggc aaccacgacg
    13261 aactgctcga tttcggggag ggagagcctg ggatggagag ggccgcgggg cgcgcgaagc
    13321 cctgcggcca tctccgccgg agcgtgtgct caaccgccgc ccagcccgac cggagccggg
    13381 gcggtgttga cgatgcgggt gaacagcgcc gcgtacttgc ccgccggtcc ctgccagggg
    13441 ttgtcgttgg cgaggaaggc ggcgtcgatg tgcgcccagt cctccgcgct caggtgcttc
    13501 cgggcgagcg ggaagatcag gtgctcctcg gtgttcatgt gctgccactc gtgctcgatg
    13561 taggtcttga gcgcgccctg gaagcccgcc aggccggatt cgccttcgcg ccggtaggcc
    13621 tcgagcgccg cacgcaaggt ggccatgcgc gcatcgccct cgcggtgctc ggcctccagg
    13681 cgctcgatga tcggcagcgc ctccgcgctg cgctcgcgca ggcgggcgaa gaggtagctg
    13741 tcctccttgg ggtggtgcac cttctccggg accatctcga tgtactcgat catcgacgcc
    13801 atcagctcga aatcgggctt cagccgcccc gcctcgatcg aggcgacgag cccgcgcaac
    13861 ccgctcagca tcgcggcgag ggcgcggtgc tcgtcatgga tgatgtccag cgacttgttg
    13921 ctcatgcttg gtttcctcct tgctctcaga cgccgagatg cttctggatc agctccggct
    13981 gggcgcggat ctcgtcgctg gtgccgtcga atacgacctg ccccttgacc aggatcatcg
    14041 cgcggtcgca gatgctggtg accgcgccgt ggttcttgtc cacgatcacg gtggcgatgc
    14101 cggtcttgcg gatctccttg acgatgcgcc agatctccat cgcgatcagc ggcgcgaggc
    14161 cctcggtggc ctcgtcgagg atcagcagat cggggttggt catcagcgca cggccgatgg
    14221 tgagcatctg ctgctcgccg cccgagagct gccagccgcc gttgttgatg cgttcgccca
    14281 ggcgcgggaa ggtctccatc acgcggtcga aggtccactc gcggcgacca tcgacgccgg
    14341 cgcgcgccga catcagcagg ttctcgcgca cgctcaggct cttgaagatg ccgcgccctt
    14401 ccggcacgta ggcgatgccc tgctgcgcgg tctggaacgt gcgcgccctg gtgtagtcgt
    14461 cgccgcgcac gagcacccga ccgtgcctgg ggcggacgat cccgagcatg ctcttgatca
    14521 gcgtggactt gcccatgccg ttacggccca tgagggcgat gccctcgcct tcgcggatgt
    14581 tgaagtcgat gccgtgcagg atgtgactcg caccgtagta ggtgtggatc tccttggctt
    14641 cgagcagcat gttagacatg gaagctgtcc tccgtaccca gataggcctg ctggacggcc
    14701 acgctggcct tgatctgcgc gggcggcccg cactccagca gctggccgtt gaccatcacc
    14761 gtgatcatgt cggcgaccgc gaacaccgca tccatgtcgt gctccacgag caggatgccg
    14821 cgcgtctggc gcaggcgctt gagcacctcg accatgctcg ccgactcctc cgaacccatg
    14881 ccggcgagcg gctcgtcgag cagcagcacc tgcgcgtcgg tggccagcac catggcgatc
    14941 tcgagctggc gctgctcgcc atggctgagc acgcccgaca cccagtcgac gcgcttgccc
    15001 aggccggcct cctcgatgca ggcgcgcgcc ttctccagcg cccacttctg ctccatcgcc
    15061 ttgctgaaga ggcgccacgg ttgctggcgg cgcgactgcg cggccaggcg cacgttctcg
    15121 agcacggtga actgcgggaa gatgttggtg cgctggtagc tgcgcccgat ccccacctgc
    15181 gagcgctgcg ccgcggtaag cgccgtgatg tccctgccga ggtagtggat gcgcccctcg
    15241 gacggcggca ggtcgcccga aagcatgttg atgcaggtcg acttgccggc accattgggg
    15301 ccgagcagca catggatcat ctggcgctcg agcgtcacgc tgatgtcctg gttcgccacc
    15361 agaccgccga agcggcgggt cagcttgtcc gcctgcagca ggacttcgtt cctcgtcgtc
    15421 acgctcattt ggcgtctcct ccctgcaggg cgcgcttgat acggcccgga acgccggcca
    15481 ggccacccgg caggaacagc acgaccgcga cgatcacgcc acccatcagc agctgccagt
    15541 acttggtgat gtcggcgaag atctcgcgca tcgcctcgaa ggcgaaggcc cccgcgatcg
    15601 cgccggcgag attgcccatg ccgccgagga tcaccatcag cagcacgttg cccgactcgt
    15661 gccaggacag cagctccggg gtcacgaagc cggacagcac ggcgtacaga aagcccgcca
    15721 gcccgccgag cgcgccggcc agcgtgaagg cgccgagctt gtagcggaag gtgtagtagc
    15781 cgagcgactg catgcggtgc tcgttgctct tgatgcccac cagcacgcgg ccgaagggcg
    15841 actgcagcac gcgcgccagg aacagataca ccagcaccag caccgcgagc acgaaataga
    15901 acatgtgcac ttcgttggtg aggtcgaacg gctcccagcc gaagatggac gccgagggct
    15961 tgttgttgag gtacttgccg tccgaaccgc cgccgagcgg ggtgtcgtgg aagatgaagt
    16021 aggccatctg cgcgaaggcc agcgtcacca tgatgaaata gatgcccttg gtgcgcagca
    16081 cgaagagccc gatcacgaag gcggcgagcg cggacaggcc gaccgaggcg ggcagcacgt
    16141 accacaggct ggccgcctcg tactcggggc tcaggatcgc caccgcgtag gcggcgatgc
    16201 cgaagtaggc cgcatggccg aagctgacca ggccggtgaa gcccaccagc aggtccaggc
    16261 tcatggcgaa gatcgccatc accagcacct tgccgaccat ctcgatgtag aaggactcgc
    16321 ccgacagcgg cacgcaggcc acgatcgcga gcgcgaccag cttgggaatc agggaagcat
    16381 ttgcgttcat ggtctcagcc cttcttcagg atgccttcgg ggcgccacag caggaccacc
    16441 gccatgacca catacaccac gatgccggag agctcgggga ccaggacctt gccgaaggtg
    16501 tcggcgaagc ccacgatcag cgcggcgacg agcgcgcccc acaccgagcc gatgccgccg
    16561 atgaccacga tcacgaagga gatgatgagc acgtggctgc ccatgttcgg gaacaccgac
    16621 gagatcggcg cggccagcat gccggcgagc gcggcgagcg cgacgccgag cgcgaacacg
    16681 acccggtaga tcatgttgat gttgatgccg agcgcctcga ccatgccgcg gtcgtcgttg
    16741 ccggcgcgga tcatcatgcc caggcgggtg cgctggatca ggtagtagag cgcgaccgcg
    16801 agcacgatgc agatcgccga gatggccagc cggtataccg gatagctcat gacctcggtg
    16861 agctggatcg aggcgtcgag gaaggcggga atcgccaccc cgtgcacatc gtcgcccacg
    16921 agcaggctgc gcagctgctc gaagatgaga atgaggccgt aggtgagcag cacctgctcg
    16981 aggtggtcgc gcttgtacag gtgggagaac agcgcccact ccaggaaggc gccgaaggcc
    17041 agcgccagcg ggatgccgag caccaccgcc agcaccaggc tgccggtcgc gctggtgagc
    17101 acgaaggcca ggtaggcgcc gatcatgtag aaactgccgt gcgccagatt gatgatgccc
    17161 atgatgccga acaccagcgt cagtccgctt gccaccagaa aaagcagcaa tccgtactgc
    17221 agcgagttca gcgtctggat caggaagctt gcgaaatcca tgacactccc cctcccggca
    17281 ggcatgcgcc tgtcaggatc gatcagggtt caagaaggaa cgggattggg gggcggcccg
    17341 agcggccgcc cctgcgaggc tgaaggctta catcttgcag ccgcgggcgg gatcctcgag
    17401 gcccttggag acgacctcga gcatcacgtt ctgatcaccc ttgacctggc gcatgtagat
    17461 gtcctgcacc gggttgtgcg ccttggacag cgtgaaggcg ccgcgcgggc tgtcgatctt
    17521 ggcggactcc atgcccttga tgatctcttc ctgcttctcc ggatcgccgc cggcggcctt
    17581 caggccggcc tcgtacatct gcgcggcgtc gtagccctgc accgcataga cgtcgggctg
    17641 gagcttgtag gtcgtggcgt acttggtgcg gaaggccgca tccttggcgt gggtcaggcc
    17701 gtcggcgtag tgcagcgtgg tgagcaggcc ctcgcccgct ccgcccatcg caccgaggtt
    17761 gccgtcggtg aggaagcccg ggccgtacag cgggatgctg ttcttgagac cggccgcggc
    17821 ataatcggcg acgaacttgg cggcgccggc gccggcgaag aagacgaaca ccgcatcggg
    17881 cttggccgcg gcgatctcgg tcaggaaggg ctggaactcg acgttgggga acggcaaggt
    17941 catttccttg acgagcttgc cgcccccctt ctcgaaggct tccttgaagc cggcgaccga
    18001 ctcctcgccg aaggagtact tccaggtcac cgtggcgacg ttcttgatgc ccttcttcgc
    18061 aagcgcctcg cccatcgcgt aggacggctg ccaggccgag aaggacgagc ggaagatgta
    18121 gggcgagcac agcgggccgg tcagctcgtt ggcgccggcg ttggggacga tcatcagggt
    18181 cttggtgtcg cgggccacct tggccatggc gagcgcgacc ccggagtgga cggtaccgac
    18241 cagcacgtcg accttgtcgc gcgagaccag cttcttggcg ttctcggtcg ccttggccgg
    18301 gttggattcg tcgtcgacgg tgaagtattc gacctcgcgg ccgccgagct tgccgccgtt
    18361 ctcgtcgacg aactgcttga agccgttggt gatcgcggtg ccgagcgcgg cgtaggtgcc
    18421 ggtgtagggc agcatcaggc cgaccttgac cttgtcggcc gcgttcgcgg cgccgatgcc
    18481 ggcagcggcc agcgcgaggg tcagggcgag gttgccggcg agtttgaacg tgctcttcat
    18541 gatgtctctt ccttgcaggt gggttggatt cgtgcgtctt ctggacgacg catcgtcttg
    18601 cggattcggt cggccccgtc gcggcacggg ctgtgccgcg acggggctgg aaacgcggcc
    18661 gcctcggcgg ccgcgcgccg acgagggcgg aggcctcaga tgaaacgggt gctgacgctg
    18721 ccgagcgact ggatgcgcag gttgacgctg tcgccggcct gcaccgagac cgcctcggtg
    18781 acgccgcccg acaggatcat ggtgcccgcg gggatctcct cgccgcgcgc gccgaggtgg
    18841 ttggccaggg ccgcgatcgc cgccgccggg tgaccgagca ccgcggcgcc ggcaccgacc
    18901 gccaccggct cgccgttctt ctcgaggacc acacccaggg tgcgcagatc caggccggcg
    18961 accggcgccg cctggccgcc gagcacgaag cgggtcgacg agcagttgtc cgcgatcacg
    19021 ctcttgaggt cgaacttgaa gtccttgtag cgcgaatcga tgatctcgat gccgggcatg
    19081 acgaagtcgg tcgccgcgag caccgcgccg atgtggcagc cggggccctt gagcgcgcgc
    19141 ttggtgacga acacgatctc gggctcgacc ttcgggtgga tcagctcgct ggtcttgatc
    19201 tcgccaccgt ccggcacgct gaagtagtcg acgagccagc cgaacaccgg ggtgtcgacg
    19261 cccatctgct tcatcttgcc gcgcgaggtc aggccggcct tgaggccgac cacgcggttg
    19321 ccgcgtgcca gcttgcggcg caggatctcg gcctggatcg cgtaggcgtc gtcccagtcc
    19381 atgtcgggat gggcctcggt gatcttcacc acttcgaagg cctgcagttc ggcgttctcg
    19441 aggtgttcgg ccagttgttc gatggtttgc tgattcagtt tcattggaag tttcctgttg
    19501 gattgggggc gggtcagacg aagcgcaccg agcagccgcc gatgccgccg atcgtcatgc
    19561 gcaggttgtc gccgcgcgcc accgggacca tggcgcccat ggcacccgag agcacgatgt
    19621 cgccggcctt gagcgcgatg ccgagcgagc cgagcatgtt ggccagccac accatggcgt
    19681 tgaccggatg ggccatggtg gccgcgccgg cgccggtgac gacgatctcg ccgttcttct
    19741 ccagcaccat gccggcgagc gcgaggtcga ggtcggcgat gtcgaccagc tggtcgccga
    19801 gcacgaacac gccgcacgag gcgttgtcgg ccacggtgtc ctggatcttg atcttccagt
    19861 cctggatgcg cgaatcgacg atctcgaagc aggccatcac gccttcggtc gcagcgagca
    19921 cgtcggccgc ggtgacgccc ggaccctgca ggtccttctt gagcaggaag gcgatctcgc
    19981 cctcggcctt gggctggatg agcgtgtcca tgtcgaccga ctcgccctcg ttgaagacca
    20041 tgctgtcgag caggtagccg aagtcgggct gatgcacgcc cagcatgttc atcaccgcct
    20101 tgctggtgac gccgatcttc ttgccgatga cgcggtcgcc ctgctcgagg cggcgcgcga
    20161 tcatgcgctg ctgcacgtgg taggcgtcct cgatcgtcat gtcgggatgc gcggcgctca
    20221 gcggcgcgat cggcgtgcgc gcgacgagcg cgtcgtagag cgaatcgccc agggcttcga
    20281 tggttttctg gtccatgtac gatcttccta ttacagcttg atgcagacgt tcttgaggtc
    20341 ggtgtagaac tcgagcgagt gcacgccgcc ttcacggccg atgcccgact gcttggagcc
    20401 accgaagggg gtgcgcaggt cgcgcaggaa ccagctgttc acccacgaga tgcccacttc
    20461 catcttctgc gcgacgcggt gggcgcgcga gacgtcctgc gtccagatcg cgcaggccag
    20521 gccgtacttg ttgtcgttgg ccttctccag cacttcctct tcggtgtcga agggggcgat
    20581 gtggcagcac ggcccgaaga tctcttcctt gatcacgcgg gcggtttccg gcaggccggt
    20641 ccagatggtc ggctgcaccc agcagccatc ggcgagttcg cccggcatct ggggcacgcc
    20701 gccaccggtc accacggtcg caccttcttc cacggcgacc ttgtagtagg acagcacctt
    20761 ctcctgatgc ttcttgctga ccagcgggcc gaagttggcg gccggatcgt ccggcacgcc
    20821 gatcttcatg ccttcggcgc cggccttcag ggcggcgacg aacttgtcga agatcgggcg
    20881 ctcgacatac acacgctcgg tgcccaggca gacctggccg cagttcagga agacggaacg
    20941 cagggtgcct tcgatggcct tgtcgaagtc gcagtcggcg aacacgatcg cggcgttctt
    21001 gccgcccatt tccagcgaca ccgggcgcga gccgttggcc gccgccttca tgatgacctc
    21061 gccggtgcgg gtctcgccgg tgaaggtgag cgcgtcgacg tccgggtggg cggtcaggaa
    21121 ggcgccggcg gagttggcgc cgaagccgtt gaccacgttg tagacaccct tggggatgcc
    21181 gaccttgttc atcacctcgc cgagcagcgc ggcggtgcgc ggggtgtctt ccgagggctt
    21241 gacgaccacg gtgttgccgc aggccagcgc cgggccgacc ttccaggtca tcagcagcag
    21301 cggcaggttc cacgggcaga tcacgccgac cacgccgacc ggacggcgat agccgtagtt
    21361 gatcgccttg ccgccgtcgg gcgtcgtcat ctcgaagaac tcggtcggaa cgttcttgac
    21421 catgtccgcg aagaccttga agttggccgc accgcgcggg atgtccacat gcgaggccat
    21481 gctcttgggc ttgccggtgt cggcgcactc ggcctcgagg aagtcgtcga agcggttgtt
    21541 gatctcgacc accagggcct cgagcagctc gatgcgcttc tgcagcgaca tcgaacccca
    21601 ggggcccttc agcgcggcct tggcggcctt gacggcggcg tcgacttcct tctcgccggc
    21661 ttcggcgacc atgccgatca ccttgttgtt gatcgggttg cggttctcgt accacttgtc
    21721 ggtggcgacg tattcgccgt cgatgaagtt gaggatcttg tcagcgatca tgatttctcc
    21781 cgttacgttt tcgaacgttg ctggattgtg ttgccgcttt tcgtcgtggc ctcgtcgggc
    21841 gggcggcgtc ggggatgggg tcggggggtg cgtcctggcg catgcccggc gcgatgcgcc
    21901 gggcatgcgg accaggtcag gtcacgacgg tcaggaagcg ctcgttgagc ttgcggtcgt
    21961 ggtagaagat gccagcgccg acctcgtcga aggtccaggt gatcggcgcg tggtcggggt
    22021 aagtctcgta gccgccgcag aaggtctcga agcggttgcc cgagggatcg aaggcgtaga
    22081 tcgtcgagcc gcgggtgatg ccgtggcggg tggggccgat gtcgatcgac acccggttca
    22141 tcgacatgat gtcggccgcg cgcagcacct tctcccagct gtcgagcagg aaggacaggt
    22201 ggtgcagctt gcccggctcg gggtgggcga cgaaggccag gtcgtgcgcc ttgtgcgagc
    22261 acgagatgaa ggtggcgagc agggacttgc cgtcctccag cagcacgcgc tcgacgaggt
    22321 ggaagccgag cacctcggtg aacagcttga ggttctcctc gaggttcggg ccgtagagca
    22381 ggcaatggtc gaagcggtgc ggagcgatgc cgtgctcgga ggcggcgatc cacggatccg
    22441 ggttggtgta gggctggccg ttgccgacgt cggtcttttc ggcatacagc tccatgacgt
    22501 ggccggtcgg gatggtgaag cgcacgcgct cgccggtctc gagcagttcg ccggccggga
    22561 tgcgctcggt ggcgacgccg taggcctgca ggtccttgtc gagttgctcg agcgtcgcct
    22621 tgtccttgac cttgaagccg atcaggtcca cgccggcacg gtcggcctgg cgcaggatca
    22681 cgctattgtg gtcgcgctcg tcccaggcct tgaaatacac acggccggct gcatcgcggc
    22741 cggtctcgac gagcccgagc acgtccttgt aaaaggccac gctttcatcc agatccagca
    22801 cgcgcagctg gatgtggcca ggacgcatca ctccggtagt tgccatcttg catctcctct
    22861 caggggtcac ggacggggtt gcgggcgcct cgtgcgaccc gcgctccccg ggttctcatg
    22921 cggcgggtcg cgcccgcctg tttctacgac tgccttgcgt ccgcggccgc cgctgacggc
    22981 gtcgcgcaca cgttcttctt catcttgccg agcaccgcca ggcggaggtc cgacagcggc
    23041 ttcacgcggc aggcgaggac gatgccggcg gcctcctcct cggcactgac gtaggcccgg
    23101 ctcatcacgc gcttgctgta ctcgccggcc tcgacctgca ccttgcacac gccgcaaccg
    23161 ccgccgcgac agcccaccgg gatgccgcgc ttgccaagcc gctccatgcc cgcgagcagg
    23221 gtctcgtcct cacggcagcg gtaaacctcg ccggtctcga cgatcgtcac ctggtggaag
    23281 accttcggcg cgggggcgtt catgtcaggc cacctgccgt tcctgctgcg cctcgttgaa
    23341 ggccttcagg cgcgcggcct gcagttcgcg ggccgcgacg aggttcttct ccttgacgtg
    23401 gccgaagccg cggatctcgt cgggcacgct cgccagcgcc accgccgcag cgtgattcac
    23461 cgggttgagc ctggcgagga tctggtcgag cagctggacg tactcctcga tcagcttgcg
    23521 ctcggtctgg cgctcttcgg tcttgccgaa gacatcgagc gcgctgccac ggaagcgctt
    23581 caggccggac aagcgcttga aggccttgaa catccactcg ccgtactgct tcttctgcgg
    23641 gtggccggtg accggatcgg tcttggagag cagcggaggc gcgaggttgt acttcaccga
    23701 gtagccatgc gggaactgcg cgtcgagctc ggcgaggaag gccgggtcgg tgtgcaggcg
    23761 ggcgacctcg aactcgtcct tgtaggccat cagcttgtac aggtagcggg cagccgcctc
    23821 ggcgagcgcg gaggaggaaa ccgccttctg ctcgcccgca accacgcgct tgatcacctc
    23881 ggcgtagcgc ttggcgtagg cctcgtcctg gaaggcgatg agctcgggca cgcggatctc
    23941 gaccaggcgc ttgacctcgc cgctggcgcc gatcgaatcg acgatcgcac gcgcggccgg
    24001 cgacagctgc ggcgccttgt tcagcacgac cacctggcca ccggtcttct ggcgcgcgac
    24061 ctcggcctcg acgaaggcgc gatcgaccac cgccatgcgg ccccagcgga aggcggccag
    24121 actcatgtcc accgcgacgc cggactggcg gatggcgtcc tcgatcgact cggcctggag
    24181 cgggatcgtg ccggcctgga aggccacgcc gaccatgaag ttgttggtcg ccatcgcatc
    24241 gccgaacagg cccgccgcca gcgcctcacc gtcgaggaac acgttgtgct ccttgcgggt
    24301 gacgcggtcg atgccgccgg tgaggccgct ggtggccggg aacagcgcct tgcggttggt
    24361 caccatctga ccggtcgggg tctgggtggt ggacacgatc gcgatggtgc gctccggatt
    24421 gcacttgtcg aggttcttcg ggtcggtggc gttgaggatg tcgaagccca gatacaggtc
    24481 ggcacggccg tcggagatct tgttcgaacc cttgaacggg gcgtgggtga tcttgatgtc
    24541 ggagatcacc gcgccgccct tctgcgccag accggtctgg tcgaggccga tcacgtgctt
    24601 gccttcgagg cgggcggcac gcgccagcgt cgccacgacg gtcaccgagc cggtgccgcc
    24661 gatgcccatg acgtggatgc cgaagcccag gctgtcatcg accttcttga ccggcggcgg
    24721 cagctcgcgc tcgagcgccg ggatgcgacc ctttttcttc ttgggcgcgc cctcggcggc
    24781 cggggccgga tgcggggtga tggtcaggaa ggacgggcag aagcccttca cgcagctgaa
    24841 gtccttgttg cacgaggact ggtggatgcg ggtcttgcga ccgaactcgg tctgcaccgg
    24901 ctcgacagac atgcagttcg acttctcgcc gcagtcgccg cagccttcgc agacgcgctc
    24961 gttgatgacg gtgacctcga ccggctcgtc ggccttgccg cggctgcgcg cacggcgcag
    25021 ctcggcggcg cactcctggt catgcagcag cacggtgcag cccggggtgg cggcgagctc
    25081 gcgctgcgcc tcctcgatgc ggtcgcggtg ccagacctcg gtgccgccgg gcagctgcac
    25141 gccgttgaag cgcgaggggt cgtcggtggt gacgatgatc ttcttgacgc cgttggcgag
    25201 gaggtcggag accatgttgg tcaccgggac ctcgccctgg atggcctggc cgccggtcat
    25261 cgaggtgtgc gcgttgcgca gcagcttgaa ggtgatgttc accccggtcg aggccgcgta
    25321 gcggatcgcc aggctgcccg agtgcgcgta ggtgccgtcg cccatgttct ggaagatgtg
    25381 ctgcgcctcg gtgaagggcg ccatgccgat ccactgcgtg ccctcggcgc ccatgtgggt
    25441 gcccatgacc acgttgcggc ccatccacat cgccatggtg tggcagccga tgccggccga
    25501 cacgatgctg ccgtcggggg cctgggtcga ggagttgtgc ggacagcccg agcagaacca
    25561 ggcggtgcgc accgcggtcg gcagcttgtt gcgcgagtgg atctcgtcca ggcgcttgag
    25621 ccagttctcg gccgactcga tgcgggtctt gcgcgacagg cgcttcagca gcgcgcgcac
    25681 gatgacgtcg gactcgaact cgccgtagtg cggcagcagc tccttctctt cctcgtcgaa
    25741 cttgccgacg atgcgcggcg cgttggccat gccgtagagc acctgcttgg cgaacatctc
    25801 caggaaggga cgcttttcct cgatgacgaa gatctcttcc agtccctgcg cgaactcgcg
    25861 cacgatgctc ggctccatcg ggaacagcat gcccatcttg aggatgcgga tgccgtagcg
    25921 gcgcagcgcg gcatcgtcca ggcccatctc gaggaaggcc tggttgaggt cgttgtaggt
    25981 cttgcccgcg gtgaggatgc cgatccaggc gtcggggttg gggaagacga cgttgttgag
    26041 gttgttggcg cgggcgtagc gcttcaccac ctcgaggcgg cgggtgtaca gcgactgctc
    26101 catctccagc gcctcgacgc gcacgttcat gccgaggttc atgttgggcg tgaacagctt
    26161 gccgtcgaac tcgaggtcgg gggtgacgaa gttcaggcgc tcgggcgaca cgctggcggt
    26221 gccggtgccg tcggcgacgt tggtgacgat cttcatgccc atccacacgc ccgccagacg
    26281 cgacatctgg tagccgtgca ggccgtagtc gaggatctcc tgcacgttgc ccgggtacag
    26341 cgaggggatg ccgacgtgga acagcatggg ctcggactgc gagcacagcg aggagctctt
    26401 gcagctcggg tcgtcgcccg cgatcgcgag cacgccgccg ttcttggcga tgccggtgta
    26461 gttggcgtgc ttgagcgcgt cgccggagcg gtccacgccc ggcgccttgc cgtaccacat
    26521 gccggtgacg ccgtcgaact tctgcttgcc gacggtgtgc agcatctgcg tgccccacac
    26581 cgcggtggcg gcgagatcct cgttgatgcc gtccacgaag tggatgtggt tctgcaccag
    26641 cagcttctgc tgcttgatca gcgcggcgtc gagcatgccg acgggagagc cgcggtagcc
    26701 ggagacgaac atgcccgtct tgagcccgcg acgggcgtcg gtgcgcacct gatcgagggt
    26761 cagacggacg atcgcgtcga tgccgccgag gtggatgtct ccttcttcct gggtgaacgt
    26821 gttggagatt tgcttgccgg atgacatgag tgtccttccc cttttatcag cccgtctccg
    26881 ggcgctttgt ccgtctcgtc ccccacgcgc cgggcggcat ggcggaacga tctggggcgc
    26941 agattagggg gagcagccat acggcaatat ccacttcgtg cacaaccgct atccgctttg
    27001 tgcagccggg accaccctgc agagagtgga tactgatgtt gcaagaactg gatatttcgc
    27061 gccttttgac ctccctacga tgcgaactcc gcgcatcaga caacacagga gactctggcg
    27121 atgatcacca cggaatatct ggactcactc atcgaaaagg acaaggaaaa gggccttttc
    27181 cgctgcaagc gtgagatgtt caccaacagg gagctgttcg acctcgagat ggaacacatc
    27241 ttcgagggca actggatcta cctcgcccac gagagccagc tgccgaagat caacgactac
    27301 tacacgacca agatcggtcg tcagccggtg ttcatcaccc gcaacaagga caaccagctc
    27361 aactgtttca tcaacgcctg cgcccaccgc ggcgccacgc tggcacgctt caagcacggc
    27421 aacaaggcca cctacacctg cccgttccac ggctggacct tcaacaactc gggcaagctg
    27481 ctgaagatca aggacccgtc cgagaccggc taccccgaga gcttcaactg cgagggctcg
    27541 cacgatctca agaagatcgc gcgcttcgag tcctaccgcg gcttcctgtt cggcagcctg
    27601 aaggccgacg tgcagccgct ggaagacttc ctcggcgagt cgaagaagat catcgacatg
    27661 gtcgtcgacc agtcgcccga gggcctggaa gtgctgcgcg gctcctcgac ctacgtctat
    27721 gaaggcaact ggaagctgca ggccgagaac ggcgccgacg gctaccacgt caccgccacg
    27781 cactggaact acgccgccac ccaggcgcag cgcaagagcc gcgacgccgg cgacgacatc
    27841 aaggccatga gcgccggcgg ctgggccaag aagggcggcg gctcgtactc gttcgagaac
    27901 ggccacctgc tgctgtggac gcgctgggac aaccccgaag accgtccgct gatggaacag
    27961 cgcgaccgtc tggtcaagga attcggcgaa gccaaggccg actggatgat cggcaactcg
    28021 cgcaacctgt gcctgtaccc gaacgtgtac ctgatggacc agttcagctc gcagatccgc
    28081 gtgttccgcc cgctcgacgt caaccgcacc gaagtcacca tctactgcat cgcgcccaag
    28141 ggcgagtcgg ccgaggcgcg tgcccgccgc atccgccagt acgaggactt cttcaacgcc
    28201 tcgggcatgg ccacgccgga cgacctcgaa gaattccgcg cctgccagga aggcttcatg
    28261 ggccgtgcgc tggagtggaa cgacatgtcg cgcggcgcca cgcactgggt cgaaggcccg
    28321 gacgaagaag cggacaagat cgacctcaag cccatcctgt cgggcgtgaa gaccgaggac
    28381 gagggcctct acgtcgccca gcacacctac tggcttgaag agattcgcaa ggctgccgcg
    28441 gccaagaacg cataaccgga gacccaagag atgagccttt cctacgagaa cctgtgcgcc
    28501 ttcctgtacc gcgaagcccg tcacctcgac gaccgcgagt gggacgagtg gctgtcgatg
    28561 tacgacgaga actgcacctt ctggatgccc gcctgggacg atgacggcga gctgaccgaa
    28621 gacccgcaga ccgagatctc gctgatctgg tacggccgcc gtgccggcct ggaagaccgc
    28681 gtgttccgca tccgcaccga gcgctcgagc gcgaccatcc ccgacacccg cacgaaccac
    28741 cacatcagca acgtcgaggt ggtcgagcag aacgacaaga cggtcaagct gcgcttcaac
    28801 tggcacacca tgagcgtgcg ctacaaccac accgaccact tctgcggcac gagcttctac
    28861 accctggacg tgtccggtgc gagcccgctg atcaaggaaa agaaggtcgt cctgaagacc
    28921 gactacatcc gccacgtcat cgacgtttac cacgtctgac aacgagggcg gggcaccgcg
    28981 cgccggcaac ggcacgggcg accccgcccc aaccgggaga gacaaccatg acattcaaga
    29041 tcgcgctgaa cttcgaggac ggcgtcaccc gcttcatcga ctgcaacgcc accgagaccg
    29101 tggccgatgc ggcctaccgc cagggcgtga acgtccccat cgactgccgc gacggcgcct
    29161 gcggcgcctg caagtgcaag gccgaatcgg gcaagtacga gctcggcttc tacatcgacg
    29221 acgccatgag cgaggaagag gccaagctcg gctacgtcct cacctgccag atgcacccca
    29281 agagcgactg cgtggtgaac gtgcccgcct cgtccgaggt gtgcaagatc aagcacgcca
    29341 ccctcaccgc caccgtggcc gaagtgcgcc agctctcgcc aagcacgctc tccctggtgc
    29401 tgcagggcga aggcatcggc aagctcgcct tcctgcccgg ccagtacgcc aacctcaccg
    29461 tgcccggcac cgagcagacg cgcgcctact cgttcagctc catgcccgcc gacagcaagg
    29521 tgagcttcct catccgcaac gtgcccaacg gcctgatgag cggctacctc accggcgccg
    29581 cgaagcccgg cgacaccatc aagctcgccg gcccgatcgg cagcttctac ctgcgcgacg
    29641 tcgcccgccc ggtgctgatg ctcgccggcg gcaccggcct cgcgcccttc ctcgccatgc
    29701 tcgacaagct cgtcgcggcc ggcggcagcg cgcacccggt gcacctgatc tacggcgtga
    29761 acaccgatgc cgatgtggtc gagctcgagc gcctggacgc cttcaaggcc gcactgccca
    29821 acttcaccta cgacgtctgc gtggtggccg acgacagcag ctggccgaag aagggctatg
    29881 tcaccgccca catcgagccc gcccacctca acgaaggcaa cgtggacatc tacctgtgcg
    29941 gccccccgcc catggtcgag gccgtcgccc acgacctgcg cgagcgcaag gtcgaaccga
    30001 acagcttcca ttacgagaag ttcgccgcca gcgtctgagc gctgcccatc ccgtcaggag
    30061 cgacaatggc aaaccgtttc aagaacaaag tggccgtcat caccggcgcc gcgcagggca
    30121 tcggcaagcg cgtcgccgag aagatggcca aggagaaggg caccctggtg ctggtcgacc
    30181 gctccgagct cgtgcatgag gtggccaagg agctgtccgg caaggtcgaa gtcctcagcc
    30241 tcaccgccga cctggagaag ttcgccgact gcaagcgggt catggaggcc gcggtcgaga
    30301 agttcggccg catcgacatc ctgatcaaca acgtcggcgg cacgatctgg accaagccct
    30361 tcgagcacta cgaggaggac gagatcgagg ccgaggtgcg gcgctcgctc ttccccacgc
    30421 tgtggtgctg ccgcgccgcc ctgccctaca tgcaggaaca gggcaagggt gcgatcgtga
    30481 acgtgtcctc gatcgccacc cgcggcgtca accgcgtgcc ctacggcgcg gccaagggcg
    30541 gcgtcaatgc gctcaccgcc tgcctggcgt tcgagaacgc cgagcgcggc atccgcgtca
    30601 atgccaccgc gcccggcgcc accgaggccc cgccgcgtct gatcccgcgc aacaccaagg
    30661 aacagagcga gcaggagaag ctctggtaca agcagatcta cacccagtcg atcgacagca
    30721 gcctgatgaa gcgctacggc acgctcgacg agcaggccga cccgatcctg ttcctcgcct
    30781 ccgacgacgc cgcctacatc accggcacca tcgtgccggt gggcggtggc gacctgggct
    30841 gattttcccg cgtcctctcc ctcccctcgg acgcctcggc cgcgctgcag cgatgcagcg
    30901 cggctttttt tcgtccccct gtcttgcggc ctcagaatta aaaaacttta tccatagaac
    30961 catagacagc gcttttgctt cccaataaaa tcgagattct taagtttctc gaagaatggc
    31021 cgatcaagaa gccgacttcg tccgacagag agcgagacag cctgcacgcc aggcggagag
    31081 gagaagcgca tgagcaagga acgccggttt cagacgcacg tgagcaaacc cgaatggagg
    31141 accgcgtgat gcccatccgc ttcctcaacg gccagagcaa tgtcttcgcc gccgccaacc
    31201 ccttcgaggt ctccgagtac gtgcgcgcga acgtgggctc gcacagcctg cgcctgccgc
    31261 gcgccagtga cgccagcgcc tcgctcaccc accgccgcgc cggcaccctc gacctgtgcc
    31321 gtctcagcta cggcgcgcag gcccgcgtgc tgtccgagag cctcggcgac atctaccacg
    31381 cccagttcat cctgcaaggc tattgcagct acacgctcgc caaccgcacg ctcgacctgc
    31441 ccgccggcca cgtgctggtg ctcaacccgg acgagccggt ggacctcacc tactcggaca
    31501 actgcgagaa gttcatcgtc cgcatcccct cggcgatgct cgacgacgcc tgcaccgagc
    31561 accgctggtt caagcccaac gagcgcatca agttcagccc cgagccgcag cgtttcgagg
    31621 acatcgacag cctgctgctg ctgctgcgcc tgctctgcga ggaggccgaa tccgagctgg
    31681 cgacgccgca gatgctgcag cactactgtc gcgtggtcac caccaagctg atggtgatgc
    31741 tcaagcacaa cgtcagcatg gtcgccccca cccggcacgc gcccagcttc gagcgcctgg
    31801 tgaactacat cgagcgcaac atcaagctcg atctcagcgc cgaggatctc gcccactacg
    31861 ccgggctgag cctgcgctcg ctctacctgc tgttcgagaa gaacgtgaag acgacgccga
    31921 agaacttcgt gcgccagaag aagctcgaga aggtgcattc gatcctgagc gacccgggcc
    31981 aggcctgtcc gaacgtcacc gcggtcgcgc tcgagtacgg cttctcgcac ctgggccgct
    32041 tctccgaact ttacaagtcc acctacggcg tgctgccctc gcagtcgatc cgctgccgcc
    32101 agccccagcc cgggcgctga acagcgaccg gcgcccgcag ccgggcgccg catcttccgt
    32161 accccagcct gcccccgccc tcctccgggc ggggcgcggt cacgacccca cctggagaaa
    32221 tgcgccatgg aacgcatcgc gatcaatccc cccgagctct tcagcggcaa ggccttcagc
    32281 cagacattct cgatcagcac gccgcaacgg ctgatcttcg tgtccggcca ggtcgactgc
    32341 gaccgcgagg gcaaggtgcg caaccccgac gacctcgagg cgcaactgaa aggcacgctc
    32401 gacaacctcg agatcgcact gcgcgcgcaa ggcgcgagca tgcgcgacct ggtgcaggtg
    32461 acgatctacg tcgtcgggct gcgccccgag gacaccacgc gcatccgcga gatccgctcc
    32521 gcctacttcg acgccgagcg cccgccggcg gtgaccctgc tcggcatcga gcgcctcgcc
    32581 ttccccgggc tgcgcgtcga gatcgacatc atcgcggcgc agtgacgcaa aggcccggcg
    32641 cgccgcgctc cggcgccgcc gggcaggcag ggcacgcgca tgccgtcggc gcgcgatcag
    32701 cccttgcgcg gcggattcag gaaaccgcgc atcaggcggg tgaggcgcgg catcaccacc
    32761 caggtcatcg tcaccacgat cagcgcagtg agcaccgcgg tgcgcggcag gaagggcagc
    32821 ggctggatcc acggcagcac gaaccacagg aagaaggaca cggtggggaa gatccccagc
    32881 caggtcacca ccgccatgcg cacgcgcggc ggatgcatcg tcgcgggcac caccgcgggc
    32941 tcgaaccagg cttcgagccc gctcagcttg cggaattcag gctcgccctc ggccaccgcc
    33001 ttcatgcgct cgagcagctc gtgtcgtgcc tcgctcatgt cccaggcctg caactcggcc
    33061 tccgaggcga agcggatcac gagctgatac tcctcgcccg ccgcctcggg cggaatcaga
    33121 tcgccgccca gaaaaccctt gtgctcgcgc atcttggcga gcatttcgcg cagcatcact
    33181 tcgtactcgg cctcgtgccc gggacgtgcg cggcggcggg cgatgcgggt gatgcgggcg
    33241 ggagtggcgg caggtgcatt catcgaggac tctcctgttc tgacggggcg gtgaagccgg
    33301 attgcgcatc accgcgggta gagctctact ctaacgcata attctcgaga tttttataaa
    33361 taatcgtgat taatattttg catggtcacg atcggccgct ccgcgcacac ggcagacgaa
    33421 aaaccccggc caccaggacg gtgtgccggg gttgtgcgcg cagctccggg aaaggccccg
    33481 ccgcctctta cagcggcatg atcagcttga ggatgaactt gtcgccctcg aagcggttct
    33541 ccgcgcccgt ttcgcggttc cactggccag tgaaatgcac gccgcccttg gtcgtgtagc
    33601 tgacggtggg gccgatggcg aagacctcac ccttgcgtcc ctcggaccag aatccaggat
    33661 tcgcggcaat cgtctgaccg ttgatctcgt cgtccgtggt ctgcttcagg tagtagccgg
    33721 acagacccac accccacgga ccaaagcgtt tcccgaccag atagtcgaag tggaattcgt
    33781 cgcccgactg gtaatccatc ttcgcgccac ccggtacgct ccggaaatcg ctgttctcgg
    33841 tcttgatgtt gtacatcacc ttggccgaca cctcccagcc cgtgtcgccc atgtaggtga
    33901 aagcgaagag cggttcgaag ctccagtagt tggcgccgat gctctggcgc gcatcgcctt
    33961 ccttatagcg cccgaccggc aggttgacgt ccagcccgat cgcccagtgc cagttcttct
    34021 catgccagga gacgatgaag ggattgacgg taacgtcgcc gagcccactg accgacttcg
    34081 agccacccag gtcgacctcc tggtgcacca ccggcacaat cacatgccag ccccagttgc
    34141 cgccgagaat cttgtgctca ctggtatgga cgaagcggaa agcgttgaac caggcatcca
    34201 cctccccagt cggcgccttt ttgccattac cgtcgaccag atcgccaccg tagtagccga
    34261 aatagttgat gaaatagttc cccggcggcg gcaccgcgcc ggccagccag gtctcggccc
    34321 cgttcggata ctggtccccg ccttccttgg cgtgcgcgcc gccgatcgcg agcgtaccga
    34381 gcgcgaccgc agccgcaatc ttgctgatgt tcatcccttc atctcctcct tgattggtct
    34441 tgcgacgagt gtgctgctcc tggaccggcg ccctctgc
//
LOCUS       DAOQNN010000004        29596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_438_length_29596_cov_10.254426, whole genome shotgun sequence.
ACCESSION   DAOQNN010000004 DAOQNN010000000
VERSION     DAOQNN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 29596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29596
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_438_length_29596_cov_10.254426"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PKV97_00755"
     CDS             <1..571
                     /locus_tag="PKV97_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HNB04432.1"
                     /translation="GGGLGDLLGGLLGGSAGGGTGTRSGGGNAGMAILATIAMAALKN
                     WTDGRRAQAAMAPDTAGFAAPELETMTEPATAELIVRAMISAAKADGEVSEDEIQRIV
                     GRIGADGLSEEEKQFMIAELRRPLDLAALVAEVPNEMVAAEVYAASLLAIELDTPAEA
                     AYLRQLAQALRLDGATLSRLHEITGATAI"
     gene            complement(629..841)
                     /locus_tag="PKV97_00760"
     CDS             complement(629..841)
                     /locus_tag="PKV97_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNB04433.1"
                     /translation="MSDRVYKLVEIVGTSHESTDAAIRNAIETAARTIRHIDWFEVVE
                     TRGHVVDGKVAHFQVTLKLGFRLESE"
     gene            complement(883..1029)
                     /locus_tag="PKV97_00765"
     CDS             complement(883..1029)
                     /locus_tag="PKV97_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04434.1"
                     /translation="MAFKLAVFTAIVVAIAVLGVRGYAASVRGFEAARALRGEDRSAD
                     PDAD"
     gene            complement(1182..1844)
                     /locus_tag="PKV97_00770"
     CDS             complement(1182..1844)
                     /locus_tag="PKV97_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNB04435.1"
                     /translation="MKSKVDEVFVGKVRLLSIEGERSGIVKSPAAGPVMLTPTGLEGD
                     EQADLRHHGGLEKALHHYPVEHYGVLAAEWPQCAAALGAGFLGENISTHGLDEHVLCI
                     GDVLRIGAARVQVSQTRSPCWKIDLRLQVEGASRFVEAAGITGWYYRVLQPGRIAAGD
                     TIELLERPNPWLTLADYWDTVSAHRPDAGLLARIAGAPGLAADKATRWRERAAWLRAQ
                     GG"
     gene            complement(1960..3267)
                     /locus_tag="PKV97_00775"
     CDS             complement(1960..3267)
                     /locus_tag="PKV97_00775"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNB04436.1"
                     /translation="MKPINVGLLGIGTVGGGTYTVLKRNAEEITRRAGRPIRIVTVAD
                     KNLELARKVTGGEVKLTDDAFSVVTDPGIDIVVELIGGYGVARELVLKAIENGKHVVT
                     ANKALLAVHGNEIFAAAQKKGVMVAFEAAVAGGIPIIKALREGLTANRIEWLAGIING
                     TTNFILSEMRDKGLPFAEVLKEAQALGYAEADPTFDVEGVDAAHKATIMSAIAFGIPM
                     QFDKAYIEGISKLDSVDIGYAEQLGYRIKLLGIARRRENGVELRVHPTLIPAKRLIAN
                     VEGAMNAVLVQGDAVGATLYYGKGAGAEPTASAVIADLVDVTRLHTSDPEHRVPHLAF
                     QPDQVHDVPVLPIEEVVTSYYLRMRVEDKPGVLADITRILADSGISIEALIQKQAAEG
                     EAHTDIIMLTHQTAEKNANAAIVRIEALPVVQGKVVKLRMEAL"
     gene            complement(3334..4641)
                     /locus_tag="PKV97_00780"
     CDS             complement(3334..4641)
                     /locus_tag="PKV97_00780"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNB04437.1"
                     /translation="MKAVKTLELAAASPEPVIKAEAGAAAAPRPIRKSAKLAEVCYDI
                     RGPVLVRAKQMEDEGHKIIKLNIGNLAAFGFDSPEEIQMDMIRNLPNAAGYSDSKGIF
                     SARKAVMHYTQQKGIKGVTIEDIYIGNGVSELIVMAMNALLDAGDEVLVPAPDYPLWT
                     AAVSLSGGKPVHYLCDESKGWLPDIDDMRAKINANTRAIVVINPNNPTGAVYPDETLK
                     QIVALAREHDLILYADEVYDKVLYEGVRHTSMAALSEDVLTIIFNGLSKNYRSCGYRA
                     GWMVVCGDKRRANDYIEGLNMLASMRLCANVPGQYAIQTALGGYQSIDDLVAEGGRMR
                     RQRDLAWELINQIPGVSCVKPQATLYMFPRLDPKVYPIEDDQAFIAELLEEERVLLVQ
                     GSGFNWPHPDHFRLVFLPHEDDLRDAIGRIARFLENYRKRHGT"
     gene            4794..5177
                     /locus_tag="PKV97_00785"
     CDS             4794..5177
                     /locus_tag="PKV97_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HNB04438.1"
                     /translation="MKLYQDKHANTNVITGYGDDHVMIGKTRHEGNVLVSAGRIVGDW
                     APQAGGDLAGLTAEDLGRAAELGAEILIVGTGRRQRFPQPPLLRGLVEARIGFEFMDF
                     AAACRTYNILVAEGRAVVLGLLHER"
     gene            5323..5778
                     /locus_tag="PKV97_00790"
     CDS             5323..5778
                     /locus_tag="PKV97_00790"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNB04439.1"
                     /translation="MSAPLAPDFSLPATAGQTVTLSALRGRKVVIYFYPKDNTPGCTN
                     ETRDFGALHAKFAAADCAVFGISRDSLKSHENFKAKLELPFELVSDPEEQACEAFGVM
                     KLKNMYGKQVRGIERSTFLIDAAGAIAREWRGVKVPGHAEEVLAAARAL"
     gene            5864..7342
                     /locus_tag="PKV97_00795"
     CDS             5864..7342
                     /locus_tag="PKV97_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNB04440.1"
                     /translation="MSALRKSATSPSLPADAGEPAIKRVRRTRKAPAACKLFVLDTNV
                     LMHDPTSLYRFEEHDLYVPIMTLEELDNNKKGTSEVARNARQASRMLDEIVCASEDGI
                     AAGIALEGPSRGQATGRLHLQTEAIDIKLPPALPTARGDNQILAVVMFLVEKHPGREV
                     ILVSKDINMRIKARALGLAAQDYFNDKVLEDSDLLYTGTCELPADFWDSHARGMQSWK
                     EEGRSYYRVSGPLAAEMLVNEFVYQDGDNPLQAWVKEKEGRSALIETLTDYSHHKNNV
                     WGITARNREQNFALNLLMNPEIDFVTLLGQAGTGKTLLTLAAGLTQVLESKRYSEIIM
                     TRVTVPVGEDIGFLPGTEEEKMAPWMGALEDNLDVLNGTAGEGGDWGRAATRDLIRSR
                     IKIKSLNFMRGRTFLNKFLIIDEAQNLTPKQMKTLITRAGPGTKVVCMGNIAQIDTPY
                     LTEGSSGLTFVVDRFKGWAHSGHITLQRGERSRLADHAAEVL"
     gene            7417..9318
                     /locus_tag="PKV97_00800"
     CDS             7417..9318
                     /locus_tag="PKV97_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HNB04441.1"
                     /translation="MSNDAADLLAGASMDFLRNFSPFNRMEPGALAFLAERAVLAFHA
                     RGSEILTPESGVPSHFYIVQRGKIQARQTGQTTVTEYTSLTLGPGECFPIGAISARRP
                     STNAYVAVEDSFCFQISADDFLHLMQISPVFHLFCTQYIASLLSQSRQQLQSTFAQRA
                     AEQQTMTTALGKLIEKEAVFVTPDTPTRSALERMSELHLGCMVVVDAERRPVGILTQS
                     DLLPRVILAGFDLARPIGELMTANPHQLPASASAYDAALEMATHGVRHLLVVDSDSVL
                     LGVVSERDLFSLQRISLRQIRAGIDNAADIEALQRASKDIRQLALNLIAQGIGAEQLT
                     QFISALNDALTRRIIILARERHELVGIDFAWLAFGSEGRHEQTLSTDQDNGIIFRCAE
                     PKQLKDTRARLVEFARTVNEDLAACGFPLCKGNIMASNPELCLTLDEWKSRFGDWIRE
                     PDPQALLNASIFFDFRVLYGNERFGDQLRSALNKGAKGNSAFLRMMAANALKVMPPLG
                     RIRDFVVDEDGTIDMKKSGARLFVDVARILALRTGVESSSTVQRLRQGSTKIGGMSPE
                     EVSAIIDGFNFIQLLRLRSQHLETEHDSAEDNRIDPESLNELDRRILKEAFRQARKLQ
                     LRLKLDYQL"
     gene            9315..9956
                     /locus_tag="PKV97_00805"
     CDS             9315..9956
                     /locus_tag="PKV97_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB04442.1"
                     /translation="MSWLSRLKNARGNQPPLSAEAERALEAWHALAESAHEQPHFESR
                     YVVLNTEATGLDLERDRLLAVGAIAVDGGLLHPQDAYHAQLEPLPGDALAGLLGFARN
                     SPLVVFNATFNRTMLERAFSRMLGFTPEFLWIDLYFLLPSLFPERLERPGRLADWMAA
                     FEIETFQRHHALGDAWAIAQLFLAAQSRALSLAVTSPRALAEHAQAYRQKIKV"
     gene            10045..10596
                     /locus_tag="PKV97_00810"
     CDS             10045..10596
                     /locus_tag="PKV97_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNB04443.1"
                     /translation="MFFRTSLPALLASFALALPAQGAEPAPAQREHAFQASSAFEEYT
                     TAAEQLVDEALSYLGIRYRFGGTSPATGFDCSGLVLNVFRNAVGLDLPRSASEMASLG
                     DKIGKQDLKPGDLVFFNTMRRTFSHVGIYLGDGKFVHAPSSGGKVRVENISATYWAKR
                     FNGARRLVDEESLGLSAQVMRTH"
     gene            complement(10654..11013)
                     /locus_tag="PKV97_00815"
     CDS             complement(10654..11013)
                     /locus_tag="PKV97_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HNB04444.1"
                     /translation="MNALLVGADRLGNIPDVLHNFGIRIAAHVSGRDTTHQRRSTPLP
                     SGIGLVILFTDFLGHNVMQRFREAAGKQGVQVVCCRRSTCALQQALDRQMGSRCDECP
                     RRCEKASGGRRRASSAC"
     gene            11131..12144
                     /locus_tag="PKV97_00820"
     CDS             11131..12144
                     /locus_tag="PKV97_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB04445.1"
                     /translation="MSKKVLIASIIAAFGLTGTVTAAELNVYSARHYQTDEELYGNFT
                     KQTGIKINRIEAKEDELLERIRNEGANSPADIFVTVDASRLAKADELGIFRPVKSAAL
                     EARIPAHLRAPNWFSYSTRARVIVYNPDMVKAEQVQTYEQLADPALKGQVCTRSGSHP
                     YNLSLGAAMIKHNGAEATENWARGIVANFARAPKGGDTDQIRAVAAGECGVAIANSYY
                     LARLMNSDKREDQAVVAKIKAVWPNQATWGTHINVSGAGMLEHAPNKEAAVKFLEYLA
                     SDQAQEYFANGNNEWPAVPSVKVDNPALKKLGEFKADTLPIGELADTVAEAQRIFDRA
                     GYR"
     gene            complement(12135..14267)
                     /locus_tag="PKV97_00825"
     CDS             complement(12135..14267)
                     /locus_tag="PKV97_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HNB04446.1"
                     /translation="MATSVLHRFRRPLQATGLLGVLFVLSVFMPPVRLFADARDYLPL
                     HSGLEIFSLVVAGMVFALGWNLRRSARGARLVWLGAACLAVGLLDFAHLMSFPGMPPF
                     FTPNSADKAIAFWLMARLLGVLGLLAHAVLPERDGGGPAAAWAVLAAIGVAFGWSWLV
                     LAHPQVLPATFIEGQGVTPLKSVLEGGLVLLHLAAAILLARRALADGESAAGWLAAAA
                     ATMALAELHFARYAAVADLYNVLGHAYKVAAFAMIYRAVFVSGVRAPQRALAAERALL
                     RGLIDSVPDLIAFRDREGRYLGCNKAFAEAYGIREAALVGRSDGEVFAGRLPLAGAGQ
                     DTVSCAENHEEWLEAADGTLRLLDTLRTPYFDLDGVQLGEIRVSRDFTERRRVREQIA
                     ERERRLMLALEGAELGLWDWDVPSGRANFSPMWCRMLGYAADELPANVSSWESLVHPD
                     DWYDIRDSLEPHLRGTKDAYRAEYRLKHKSGHWVWVLAAGRVLDRGAGGEALRMVGIH
                     QDISQRKAMEESLLQLATSDPLTGLWNRRHFVEVVRGELGRVRRNQAPAALLLMDLDF
                     FKRINDTHGHAAGDEVLRHFTATVSAQLREADVFARLGGEEFAVLLPSIDALGAVRAA
                     DRLRRVVADSPASVDSGALHYSVSIGATMLDAADEGYEAAYARADEALYAAKHAGRNR
                     VVLAPASAGAPCGEPVSEGAAAGAADQR"
     gene            complement(14477..16117)
                     /locus_tag="PKV97_00830"
     CDS             complement(14477..16117)
                     /locus_tag="PKV97_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNB04447.1"
                     /translation="MRIRFSSLTVLAVVVATLIAAPVLSVFSNVLAGDTGATWSHLAD
                     TVLGEFVFNTVVLCIGVGLGVSSIGVTSAWLTTMLDFPGRRFLEWALVLPLAMPAYVM
                     AYVYTDFLQFVGPVQGWLRETFGWGRGDYWFPDVRTVGGAVAMFMFVLYPYVYMIART
                     AFLERAGGMLEAGRTLGLGPWGSFFRVSLPLARPAVVAGTALALMETLADFGTVSYFG
                     VQTFTTGIYRAWFSLGDRIAAAQLSAALLAFVVLVLTLERMSRGRARFNNTSRQVAMP
                     VRLRLPLALGVLAAFACFMPLLLGFLLPAALLLRMAFLEGDAQFGARFVELAHNSFVL
                     AAITAVVAVLLAVLLAYAARLARSRLPQVLNRIVGLGYAVPGSVIAVGVLIPLTRVDR
                     WLAQGWEALWGTNPGLLLTGGIAALIYAYLARFLAVALQTVESSLGKITPNMDDASRS
                     LGFGKWATLRRVHMPMLRGSLLTAGLLVFVDVMKELPATLVIRPFNFDTLATQAHTLA
                     ADERLAEASTAALIIVAVGLLPMFWISRQILRVRRREG"
     gene            16271..17380
                     /locus_tag="PKV97_00835"
     CDS             16271..17380
                     /locus_tag="PKV97_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB04448.1"
                     /translation="MSHLDLSRIDLAFGSHLVVRQLSLQLEKGHIGCLLGPSGCGKTT
                     VLRCIAGFERLAAGEIRLDGEVVSTPHHMLPPERRRIGMVFQDYALFPHLSVADNIGF
                     GLRRDSAAVRNARVDELLALVGLAGQGRKYPHEMSGGQQQRVALARALAPKPSLLLLD
                     EPFSNLDVELRERLSYEVREIIKAADTTAILVTHDQHEAFAVADEIGIMHEGRIQQWD
                     TPYNLYHRPANRFVADFIGQGAFLRGKVLDDHRVQIELGVLDSTQPLVCCADCAGCVH
                     GSPVDVLLRPDDLVHDDASALRAEVVHKAFRGADILYTLRLAGGARVLSLVPSHHNHA
                     IGERIGIRLDVDHVVTFSDERADECAPAHPAASLA"
     gene            17377..18084
                     /gene="aat"
                     /locus_tag="PKV97_00840"
     CDS             17377..18084
                     /gene="aat"
                     /locus_tag="PKV97_00840"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNB04449.1"
                     /translation="MIPWLGAEPGFPADERALREPNGLLAAGGALTPPWLLAAYRRGI
                     FPWFSEGEPILWWTPDPRLVLVPDEFHISHSLRKTLRRRRFEVRTDTAFAEVLLACAA
                     ARAGGPGTWITPAMRAAYQRMHELGWAHSVECWREGRLVGGLYGMAIGRVFFGESMFA
                     RETDASKVALAHLARLLAERGYGMIDCQMTTAHLCSLGAREVPRARFTAALRQWAHDG
                     PAPQRWAERTMQDFDWN"
     gene            18115..18843
                     /locus_tag="PKV97_00845"
     CDS             18115..18843
                     /locus_tag="PKV97_00845"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNB04450.1"
                     /translation="MKSDYPYALIQFYATAPYACSYLAGRSARSQVATPGHLIDPQVY
                     SELVRRGFRRSGVFTYRPYCDHCQACVPVRVPVERFQPDRSQRRTWARHADLIPIERP
                     LEFVEEHYQLYQRYQTARHAGGGMDLDNREQYAHFLLQSHVDTRLVEFRSYGSLRMVS
                     VIDRLTDGLSSVYTFYDPDQPRSAFGTWGVLWQIEACRVLGLPHVYLGYWIGESPKMA
                     YKARFRPLEALIDGDWQPFPGDFA"
     gene            18886..19893
                     /locus_tag="PKV97_00850"
     CDS             18886..19893
                     /locus_tag="PKV97_00850"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNB04451.1"
                     /translation="MLYELARPLLFALDPETAHNLTLHGLHYAGKLLPAAEPEPASAV
                     EVMGLRFPNRVGLAAGLDKNGEAIDGLARLGFGFLEIGTITPRPQPGNPRPRMFRLPE
                     VQGIINRMGFNNHGVDVLLAHVRAAKYRGILGINIGKNFDTPIERAADDYLACLEKVY
                     ALASYVTVNISSPNTKNLRQLQGESELDDLLGRLKAAQTRLTEQHGRYVPLTLKIAPD
                     LDDAQVSNIADALRRHRIDGVIATNTTIARDKVQGIAHGNEQGGLSGAPVFEASTAVV
                     RKLAHALAGELPIIAAGGVLEGAQARAKLEAGAALVQLYSGLIYRGPALVRECVRATA
                     G"
     gene            19991..21079
                     /locus_tag="PKV97_00855"
     CDS             19991..21079
                     /locus_tag="PKV97_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB04452.1"
                     /translation="MTHRRQFVLSAAALAVAATLPFAAHAADAAKVGFVYVSPIGDAG
                     WTYQHDLGRKELEKTLGGRIETRYVENVAEGADAERVIREFAASGSKIVFATSFGYMN
                     YVERVARQFPNVTFLHATGYKSAKNFAPYNARFYEGRYLNGVIAGKMTKSNVLGYVGA
                     FPIPEVLQGINAFTLGARSVNPNVEVRVIWANSWYDPGKEREAAMTLISQGADMLTHH
                     TDSTATVQAAEEKGVYAFGYHSDMSKYAPKSQLTATTHHWGEFYARTVDAVLGGTWKP
                     EGVWGGMKDGMIRLAPLNPAIPADVQAMVKELQGRITAGSLHPFTGPVLDQAGKERLA
                     AGAVMDDATLGKMDYFVQGVGSRLPTAK"
     gene            complement(21156..22205)
                     /locus_tag="PKV97_00860"
     CDS             complement(21156..22205)
                     /locus_tag="PKV97_00860"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNB04453.1"
                     /translation="MFRAIDFVKIPLMEYSSSLVSFCQEGLGSPAWVRGTTVHGNADG
                     GRIVTAGTRALHDLNQDVARVELFHPHGRSLCGSLQASGHARTGDAWRRAPRHGLLAL
                     VLAAIAGGALAGGEGPGPVAVDVGHTLDAPGATSARGRSEFEFNRDLSVHVVDALHAR
                     GWAVSTINADGRIASLRARPQAAGEAGAAFLLSLHHDSVNAVELRPWTWEGRALDYND
                     EFAGHSLFVSRDNPDTAMSILCARAIGARLQRMGFEPTHKNGRRRSYADREHAVHHYD
                     GLAVLRHARMPAVLFEAGILKNRDEELLLRDPERQARMADAIATGISACLRNGLPADD
                     EAWADRPDRADPPTP"
     gene            22168..22593
                     /locus_tag="PKV97_00865"
     CDS             22168..22593
                     /locus_tag="PKV97_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB04454.1"
                     /translation="MSGIFTKSMARNIFYGGTTFFFLLFLALTFDTERELPNRDNRAA
                     ITPEVAAGKHIWETRNCLGCHTLLGEGAYFAPELGNVYKRRGGDFIKGWMQAMPTGAP
                     GRRQMPQFNLTDEELDQLVAFLKYTSEINTANWPPNIEG"
     gene            22625..24007
                     /locus_tag="PKV97_00870"
     CDS             22625..24007
                     /locus_tag="PKV97_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB04455.1"
                     /translation="MQYKSQAVAMPYFIAAIGLFVGQIIFGLILGLQYVVGDFLFPEI
                     PFNVARMVHTNLLIVWLLFGFMGAAYYLIPEETETELFSPKLALAMFWIFLVAGALTI
                     VGYLLVPYSTLASMTGNDILATMGREFLEQPLLTKIGIVIVALAFLFNITMTVLKGRK
                     TVISIVLLLGLWGLAIFFLFSFYNPVNLVRDKFYWWWVVHLWVEGVWELIMAAMLAFV
                     LIKVTGVDREVIEKWLYVIITLALVTGIIGTGHHYFWIGTPEYWQWWGSIFSALEPIP
                     FFAMTVFAFNMVNRRRREHPNKAAVLWALGTGVMAFLGAGVWGFLHTLAPVNYFTHGS
                     QITAAHGHMAFYGAYVMVVITMISYAMPILRGRAANSTKAQVLEMWAFWLMTIAMVFI
                     TLFLTAAGILQVWLQRVSDTPMSFMATQDQLMLFYWMREWAGVMFFIGLLAYLASFFV
                     KGDAKGVVHG"
     gene            24273..25319
                     /locus_tag="PKV97_00875"
     CDS             24273..25319
                     /locus_tag="PKV97_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNB04456.1"
                     /translation="MHPSGVERPFRIAINGYGRIGRCYLRALHEAGLAERYRIVAINE
                     PADLASIAYLTRFDSTHGRFPGVVERSERELIVDGHAIHVSHASTPEEVDWTVLGLDL
                     VVECSGQYGGRRDLQRFIAAGCPRLLLSHPGNGAEDVDVTVVFGINEDELHGPERLVS
                     NASCTTNAVVPVLDCLHREIGIEQALLTTLHSAMNDQPLIDGYHHADLRRTRSAMQSI
                     IPVSTGLARGVERLLPALAGRVQAKAIRVPTHNVSAIDMVLSFGREVSAEAVNRLLRE
                     TAAGPQRGLLACSDEAHASIDFNHDPHSAIIDCSQTRAVGAKMLNLVVWFDNEWGFAS
                     RMVDVTRRWLAAAG"
     gene            25386..26000
                     /locus_tag="PKV97_00880"
     CDS             25386..26000
                     /locus_tag="PKV97_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNB04457.1"
                     /translation="MNTVTTPPSSLPAGGPAQAHGPRLAGDLAIWFFILAELLAFGVF
                     FAAYAFARAKNIELFAAEQAALNRNAGALNTVLLLTASYFVVRAVQAAEAQASRQCAN
                     WLGGAILTGLGFIVVKLSEYAAAFEHNISLSSSTFHMFYLSLTFFHFMHVILGLVILI
                     AMWNGARQGRYRPGEMNGLETGAAYWHMVDLVWLILFPLVYVIH"
     gene            26011..26292
                     /locus_tag="PKV97_00885"
     CDS             26011..26292
                     /locus_tag="PKV97_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNB04458.1"
                     /translation="MSARMHGTPRGSTILFATLILATVATWGMGSLGVTGTWGVAILA
                     AISFWKGAVVILDFMALRHAPLLWRAITMGWIIVVWALIAIAYIKGLAQ"
     gene            26289..27089
                     /locus_tag="PKV97_00890"
     CDS             26289..27089
                     /locus_tag="PKV97_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNB04459.1"
                     /translation="MKPDTPNLDIPFYQPAGDEVDIFQHAWKNRLPLLIKGPTGCGKT
                     RFVSHMAARLGLPLYTVACHDDLTAADLVGRHLIGDGKTMWCDGPLTRAVREGGICYL
                     DEVVEARKDTTVVLHPLADDRRVLPIDRTGELLEAPPSFMLVVSYNPGYQNLLKGMKP
                     STRQRFVSLRFDFPRPEQEEAVLIGETACAPDLAKRLVAIANALRALKDRDLEEAAST
                     RLLVYTALLVKDGMDPVAASRVGIVESLTDDADVAEGLMEVVAATFGR"
     gene            27106..27513
                     /locus_tag="PKV97_00895"
     CDS             27106..27513
                     /locus_tag="PKV97_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04460.1"
                     /translation="MEIKRADPLRHVMPGPRLAVVAEALFLINLMLLPGLAFLALMVL
                     WYLHRGHPDPLVRNHLRQTGWTCIWGGLLLVVISVAIFLLGGFDNPWTWVIGVLYFTF
                     VHSSLILLGVMGLSRAINGRSWRFPVFGPREPS"
     gene            27531..28550
                     /locus_tag="PKV97_00900"
     CDS             27531..28550
                     /locus_tag="PKV97_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB04461.1"
                     /translation="MNTGAPARKPQRVIPIATAPAGDAAAAAAMQRRRRAFQAGFFAL
                     FVLAPVFDLFRYDLDADHAWLLGMEWRLGLDPFLAGQASALEAAGSIILNLFVPLLAL
                     AALVIGAAWKWGRLYCGWLCPHFSVVESINRLMARASGKASVWDRHLLPAWKPDGRPA
                     RVDARWWGVVVPAAVGFAFLWAVVLLTYLLPPAQVYGNLFAGELTRNQAIFLTAATVV
                     LSLEFLFARHLFCRFVCAVGLFQSLAWMGNRDALVVGFERERASDCSNCLPDRQSACD
                     AACPMRLRPRNIKRHMFTCTQCRQCVDACATTQHANPQGPLLSWVAGEAARQNEAGFR
                     ALKDR"
     gene            28555..29187
                     /locus_tag="PKV97_00905"
                     /pseudo
     CDS             28555..29187
                     /locus_tag="PKV97_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
     gene            29294..>29596
                     /locus_tag="PKV97_00910"
     CDS             29294..>29596
                     /locus_tag="PKV97_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04462.1"
                     /translation="MRELVVQHVLQQAPGFLAQRRALPRRRDDASEHVRPQHVVGVEF
                     VAPLEPDHPRAQVGELPRSAVHDREREARTEDLGLYRVVVDERHGRLSEQAGGKLRA"
BASE COUNT         4806 a        10851 c         9436 g         4503 t
ORIGIN      
        1 cggaggcggc ttgggcgatc tgctcggcgg cctgctcgga ggcagcgccg ggggcggcac
       61 gggcacgcgc agcggcggcg gcaacgccgg catggccatc ctcgccacca tcgcgatggc
      121 ggcgctgaag aactggaccg acggccgccg cgcgcaggcc gcgatggcac ccgacacggc
      181 gggcttcgcc gcccccgaac tcgaaaccat gaccgagccc gcgaccgcgg agctgatcgt
      241 gcgcgcgatg atctcggcgg ccaaggccga cggcgaagtg agcgaggacg agatccagcg
      301 catcgtcggc cgcatcggcg ccgacggcct gagcgaggag gagaagcagt tcatgatcgc
      361 cgaactgcgt cgcccgctcg acctcgccgc gctggtcgcc gaggttccca acgagatggt
      421 ggcggccgag gtctatgccg cctcgctgct cgcgatcgaa ctcgacaccc cggccgaggc
      481 ggcctacctg cgccagctcg cccaggcact gcgcctggac ggtgcgacgc tgtcccgcct
      541 gcacgagatc accggcgcca ccgcgatctg agcgcgggta tcgggccgca ccgccgcacg
      601 ccaagctggc gcggcggcgc gactcgcctc actcgctctc gagccggaag cccagcttca
      661 gcgtgacctg gaagtgcgcg accttgccgt cgaccacgtg gccgcgcgtc tccaccacct
      721 cgaaccagtc gatgtggcgg attgtgcgcg cagcggtctc gatcgcattg cggatggcgg
      781 catcggtgct ctcgtgcgag gtgccgacga tttcgacgag cttgtagacg cgatcggaca
      841 tcttgtcctc ctcttcggtg ccggccagcg ccggatggct gttcagtccg cgtccggatc
      901 cgcggaccga tcctccccgc gcaacgcccg cgcagcctcg aagccgcgca ccgaagccgc
      961 atagccacgg acgccgagca cggcgatcgc aaccacgatg gcggtgaaga cggcgagctt
     1021 gaaggccatg cgaaggcgtc ctgcaggcaa gggttgggaa tcgcggcacg cgcctcgcgc
     1081 gcgccgccgt gcttcagtct aggcgcaaac gcctgtggtg tcgcgcgtgc ccggagctcc
     1141 cgccaccgcc cacgcgccct cccatctcgc cggccacctc ctcacccccc ctgcgcgcgc
     1201 agccacgccg cacgctcgcg ccagcgcgtc gccttgtcgg cggcgagccc gggcgcgccg
     1261 gcgatgcgtg cgagcagccc ggcatcgggc cggtgcgcgc tcaccgtgtc ccagtagtcg
     1321 gcaagcgtga gccagggatt gggacgctcg agcagctcga tcgtgtcgcc cgcggcgatc
     1381 ctgcccggct gcagcacgcg gtagtaccag ccggtgatcc ccgccgcctc gacgaaacgc
     1441 gaggcgccct cgacctgcag acgcaggtcg atcttccagc acggcgagcg cgtctggctc
     1501 acctgcacgc gcgccgcgcc gatgcgcagc acgtcgccga tgcacagcac gtgctcgtcc
     1561 agcccgtgcg tgctgatgtt ctcgccgagg aaacccgcac ccagcgcggc tgcgcactgt
     1621 ggccactcgg cggccagcac cccgtagtgc tcgaccggat agtggtgcag cgccttctcc
     1681 aggccgccgt ggtggcgcag gtcggcctgc tcgtcaccct ccagcccggt cggcgtcagc
     1741 atgaccgggc ccgcggccgg cgacttgacg atgccgctgc gctcgccctc gatcgacagc
     1801 aggcgcacct tgccgacgaa gacctcatcc accttgctct tcatgtcccc catcctccct
     1861 gccttcccgg tggcagcaat ggcgacccgg gatgacgtga acgcaagagg ccgcacccgg
     1921 gggcacggcc tcgccttcga tcacgactcg aaactgcgct cagagcgctt ccatacgcag
     1981 cttcacgacc ttgccctgca ctacgggcag cgcctcgatg cgcacgatgg cggcattggc
     2041 gttcttttcc gcggtctggt gggtcagcat gatgatgtcg gtgtgcgcct cgccctcggc
     2101 cgcctgcttc tggatcagcg cctcgatcga gatcccgctg tcggccagga tgcgggtgat
     2161 gtcggcgagc acgcccggct tgtcctcgac ccgcatgcgc aggtagtagg aggtcacgac
     2221 ctcctcgatc ggcagcaccg gaacgtcgtg cacctggtcg ggctggaagg ccaggtgggg
     2281 cacgcggtgc tcggggtcgg aggtgtgcag gcgggtgacg tcgaccaggt cggcgatcac
     2341 cgccgaagcg gtgggctcgg cgcccgcgcc cttgccgtag tagagcgtcg cgccgacggc
     2401 gtcgccctgc accagcaccg cattcatcgc accctcgacg ttggcgatga ggcgcttcgc
     2461 cggaatcagc gtggggtgca cgcgcagctc gacgccgttc tcgcggcggc gggcgatgcc
     2521 gagcagcttg atgcggtagc cgagctgttc ggcgtagccg atatccaccg agtcgagctt
     2581 gctgatgccc tcgatatagg ccttgtcgaa ctgcatcggg atgccgaagg cgatcgcgct
     2641 catgatcgtc gccttgtgcg cggcgtcgac gccctcgacg tcgaaggtcg gatcggcctc
     2701 ggcgtagccg agcgcctgcg cttccttgag cacctcggcg aagggcaggc ccttgtcgcg
     2761 catctcggac aggatgaagt tggtggtgcc gttgatgatg ccggccagcc actcgatgcg
     2821 gttggcggtg aggccttcgc gcagcgcctt gatgatcggg atgccgcccg cgaccgcagc
     2881 ctcgaaggcc accatcacgc ccttcttctg cgccgcggcg aagatctcgt tgccatgcac
     2941 cgcgagcagc gccttgttgg cggtgaccac gtgcttgccg ttctcgatcg ccttcagcac
     3001 cagctcccgg gccacgccgt agccgccgat cagctcgacg acgatgtcga tgcccggatc
     3061 ggtgaccacc gagaaggcgt cgtcggtgag cttgacctcg ccgccggtca ccttgcgcgc
     3121 gagctcgagg ttcttgtcgg ccaccgtgac gatgcggatc ggacggccgg cgcgccgggt
     3181 gatctcctcg gcgttgcgtt tgaggacggt gtaggtgccg ccaccgacgg taccgatgcc
     3241 aaggaggcca acgttgatag gtttcatttt gtccgtgagg tgtgaggtgt caggagggag
     3301 gagcaggcgg cgcagccgcc ttccggtggc ggctcaggtg ccgtggcgct tgcggtagtt
     3361 ctcgaggaag cgggcgatgc ggccgatggc gtcgcgcagg tcgtcctcgt gcggcaggaa
     3421 gaccaggcgg aagtggtcgg gatgcggcca gttgaagccg gagccctgca ccagcagcac
     3481 gcgctcctct tcgagcagtt cggcgatgaa ggcctggtcg tcctcgatcg gatagacctt
     3541 gggatccagg cgcgggaaca tgtacagcgt ggcctgcggc ttcacgcagc tcacgccggg
     3601 gatctggttg atcagttccc aggcgaggtc gcgctggcga cgcatgcgcc cgccctcggc
     3661 gaccaggtcg tcgatgctct ggtagccgcc gagcgcggtc tggatcgcgt actggccggg
     3721 cacgttggcg cacaggcgca tcgaggccag catgttgagg ccctcgatgt agtcgttggc
     3781 gcggcgcttg tcgccgcaca ccaccatcca gcccgcgcgg tagccgcagg agcggtagtt
     3841 cttcgacagg ccgttgaaga tgatcgtcag cacgtcctcg gacagcgccg ccatcgaggt
     3901 gtgcctgacg ccctcgtaga gcaccttgtc gtagacctcg tcggcgtaga ggatcaggtc
     3961 atgctcgcgg gcaagcgcga cgatctgctt cagggtctcg tccggataca ccgcgccggt
     4021 cggattgttc gggttgatga ccacgatcgc gcgcgtgttg gcgttgatct tcgcgcgcat
     4081 gtcgtcgatg tccggcagcc agcccttgga ctcgtcgcac aggtaatgca ccggcttgcc
     4141 gcccgacagg ctcaccgcgg cggtccacaa cgggtagtcg ggcgccggca ccagcacctc
     4201 gtcgccggcg tcgagcagcg cgttcatcgc cattacgatg agctcggaca caccgttgcc
     4261 gatgtagatg tcctcgatgg tgacaccctt gatgcccttc tgctgggtgt agtgcatcac
     4321 cgccttgcgc gcggagaaga tgcccttgga gtccgagtag cccgccgcgt tgggcaggtt
     4381 gcggatcatg tccatctgga tctcttcggg cgaatcgaag ccgaacgcgg cgaggttgcc
     4441 gatgttcagc ttgatgatct tgtgcccctc gtcctccatc tgcttggcgc gcaccagcac
     4501 cgggccgcgg atgtcgtagc agacctcggc gagcttggcg gacttgcgga tcggacgcgg
     4561 cgcggcggct gcgcccgcct cggccttgat caccggttcg ggggatgccg ccgcgagctc
     4621 gagggtcttc accgctttca tgccagtcct tcctgagagg tggaaacgcg cgccggagtt
     4681 ttcccgcacg ccaaaaggtt gatatcttag ccgcgctccc gcactgcggc aatttgcccg
     4741 ggcatcgcgg gcaaccgccg gcgtgcgccg catgccgcga ccaggaacgg acgatgaagc
     4801 tctaccagga caagcacgcc aacaccaacg tgatcaccgg ctacggcgac gaccacgtga
     4861 tgatcgggaa gacccgccac gagggcaacg tgctcgtcag cgccgggcgg attgtgggcg
     4921 attgggcccc gcaggccggt ggcgacctcg caggactgac ggccgaggat ctcgggcgcg
     4981 cagccgaact cggcgccgag atcctcatcg tcggcaccgg gcgccgccag cgcttcccgc
     5041 agccaccgct gctgcgcggg ctggtcgagg cgcgcatcgg cttcgagttc atggacttcg
     5101 ccgccgcctg ccgcacctac aacatcctcg tggccgaagg ccgcgcggtc gtgctcggac
     5161 tgctccacga acgctgagcg gcgcaacgcg gcggtaacgc cgccgcaata cgctcttcac
     5221 ccgggcaggc gctttcggct accctgaggc atacgaagca cgtgcgccgg tgcgctcgcc
     5281 ccgagcgcgc agccccgccg ccccgacgaa caggagaccc gattgagcgc ccccctcgcc
     5341 cccgactttt ccctgcccgc caccgccggc caaaccgtca ccctctccgc gctgcgcggc
     5401 cgcaaggtgg tgatctactt ctaccccaag gacaacaccc ccggctgcac caacgagacg
     5461 cgcgacttcg gcgcgctgca cgccaagttc gccgccgccg actgcgccgt cttcggcatc
     5521 agccgcgaca gcctcaagag ccacgagaac ttcaaggcca agctcgaact gccctttgaa
     5581 ctcgtctccg accccgagga acaggcctgc gaggccttcg gcgtcatgaa gctcaagaac
     5641 atgtacggca agcaggtgcg tggcatcgag cgcagcacct tcctcatcga cgccgcgggc
     5701 gcgatcgcgc gcgaatggcg tggcgtgaag gtgcccggcc acgccgagga agtgctcgcc
     5761 gccgcgcgcg cgctctgatc cttgcggcgc cggccgcgca ccgcgccccg gcgccgcctc
     5821 gcctcttcct gcggccgtcc tgccgccctc ccggatccac gtcatgtccg ccctccgcaa
     5881 atccgccacc tccccgtccc tccccgcgga cgcgggcgag cctgccatca agcgtgtccg
     5941 ccgtacccgc aaggccccgg cagcgtgcaa gctcttcgtg ctcgacacca acgtgctgat
     6001 gcacgacccg accagcctgt accgcttcga ggagcacgac ctctacgtgc cgatcatgac
     6061 gctggaggaa ctcgacaaca acaagaaggg caccagcgag gtggcacgca acgcccgcca
     6121 ggccagccgc atgctcgacg agatcgtatg cgcctcggaa gacggcatcg ccgcgggcat
     6181 cgcgctcgag ggcccctcgc gcggccaggc caccggacgc ctgcacctgc agaccgaggc
     6241 gatcgacatc aagctgccgc cggccttgcc gaccgcgcgc ggcgacaacc agatcctcgc
     6301 ggtggtgatg ttcctggtgg agaagcaccc cgggcgcgag gtcatcctgg tgtcgaagga
     6361 catcaacatg cgcatcaagg cccgtgcgct cgggctcgcc gcgcaggact acttcaacga
     6421 caaggtgctc gaggacagcg acctgctgta caccggcacc tgcgagctgc ccgccgactt
     6481 ctgggacagc cacgcgcgcg gcatgcagtc gtggaaggag gaaggccgca gctactaccg
     6541 cgtcagcggg ccgctcgccg ccgagatgct ggtgaacgag ttcgtctatc aggacggcga
     6601 caacccgctg caggcctggg tgaaggaaaa ggaaggccgc agcgccctca tcgagaccct
     6661 caccgactac agccaccaca agaacaacgt ctggggcatc accgcgcgca accgcgagca
     6721 gaacttcgcg ctcaacctgc tgatgaaccc cgagatcgac ttcgtcaccc tgctcggcca
     6781 ggccggcacc ggcaagaccc tgctgacgct cgccgccggg ctcacccagg tgctggagtc
     6841 gaagcgctac agcgagatca tcatgacccg cgtcaccgtt ccggtgggcg aggacatcgg
     6901 cttcctgccc ggcaccgagg aagaaaagat ggcaccctgg atgggcgcgc tcgaggacaa
     6961 cctcgacgtg ctcaacggca ccgccggcga gggcggcgac tggggccgcg cggccacccg
     7021 cgacctcatc cgcagccgca tcaagatcaa gagcctgaac ttcatgcgcg gccgcacctt
     7081 cctcaacaag ttcctcatca tcgacgaggc gcagaacctc acgcccaagc agatgaagac
     7141 cctgatcacg cgcgccggcc ccggcaccaa ggtggtctgc atgggcaaca tcgcgcagat
     7201 cgacacgccc tacctcaccg agggttcttc gggcctcacc ttcgtggtcg accgtttcaa
     7261 gggctgggcc cactccggcc acatcaccct gcagcgcggc gaacgctccc gcctcgccga
     7321 ccacgccgcc gaggtgctct gacgcagccg tcgcggctcg ccgctcccgc gatgcgcgcg
     7381 cctataatgt gcgattgctt cgatcccggt ccgcccatga gcaacgacgc cgccgacctg
     7441 ctggcgggag ccagcatgga tttcctgcgc aacttctccc ccttcaaccg catggagccg
     7501 ggggcgctcg cctttctcgc cgagcgcgcg gtgctcgcct tccatgcccg tggcagcgag
     7561 atcctgacgc ccgaatcggg tgtgccgtcg cacttctaca tcgtccagcg cggcaagatc
     7621 caggcccggc agaccggcca gaccacggtc accgagtaca ccagcctcac cctcgggccg
     7681 ggcgagtgct tcccgatcgg cgccatctcc gcgcgccgcc cctcgaccaa cgcctacgtg
     7741 gcggtcgagg acagcttctg cttccagatc agcgccgacg acttcctgca tctgatgcag
     7801 atcagcccgg tgttccacct cttctgcacg cagtacatcg ccagcctgct cagccagtcg
     7861 cgccagcagc tgcagagcac gttcgcccag cgcgccgccg agcagcagac catgaccacg
     7921 gcgctcggca agttgatcga gaaggaggcg gtcttcgtca cccccgatac gccgacgcgc
     7981 agcgcgctcg agcgcatgtc cgagctgcat ctcggctgca tggtggtggt ggacgcggaa
     8041 cgccgcccgg tcggcatcct gacccagagc gacctgctgc cgcgcgtgat cctcgccggc
     8101 ttcgacctcg cgcgcccgat cggcgagctg atgaccgcca atccgcacca gctgccggcg
     8161 agcgcctcgg cctacgacgc ggccctggag atggccaccc acggcgtgcg ccacctgctc
     8221 gtggtcgact ccgacagcgt gctgctgggc gtggtctccg agcgcgacct gttctcgctg
     8281 cagcgcatca gcctgcgcca gatccgcgcc ggcatcgaca acgccgccga catcgaggcc
     8341 ctgcagcgtg cgagcaagga catccgtcag ctggcgctga acctgatcgc ccagggcatc
     8401 ggcgccgagc agctcacgca gttcatctcg gcgctcaacg acgcgctgac gcgccgcatc
     8461 atcatcctgg cgcgtgaacg ccacgagctg gtggggatcg acttcgcctg gctcgccttc
     8521 ggctccgagg gccgccacga gcagaccctg tccaccgacc aggacaacgg gatcatcttc
     8581 cgctgcgccg agccgaagca actcaaggac acgcgcgccc gcctggtcga gttcgcgcgc
     8641 acggtcaacg aagacctcgc ggcctgcggc ttcccgttgt gcaaaggcaa catcatggcg
     8701 agcaaccccg agctctgcct gaccctcgac gagtggaagt cgcgcttcgg cgactggatc
     8761 cgcgagcccg acccgcaggc cctgctcaac gcctcgatct tcttcgactt ccgcgtgctc
     8821 tacggcaacg aacgcttcgg cgaccagctg cgcagcgcgc tcaacaaggg cgccaagggc
     8881 aacagcgcct tcctgcgcat gatggccgcc aacgcgctca aggtcatgcc cccgctcggg
     8941 cgcatccgcg acttcgtggt ggatgaagac ggcaccatcg acatgaagaa atccggcgcc
     9001 cgcctgttcg tcgatgtggc ccgcatcctc gccctgcgca ccggcgtgga gtcgagcagc
     9061 acggtgcagc gcctgcgcca gggcagcacc aagatcggcg gcatgagtcc ggaggaagtc
     9121 tcggccatca tcgacggctt caacttcatt cagttgctgc gcctgcgttc gcagcacctc
     9181 gagaccgagc acgactccgc cgaggacaac cgcatcgacc ccgaatcgct caacgaactc
     9241 gaccgccgca tcctcaagga agccttccgc caggcgcgca agctccagct gcggcttaaa
     9301 ctcgattacc aactatgagc tggctctctc gcctgaagaa cgcccgcggc aaccagccgc
     9361 cgctgagcgc cgaggccgag cgtgcgctcg aggcttggca tgcgctcgcc gagtccgcgc
     9421 acgaacagcc tcatttcgag tcgcgctacg tggtgctcaa caccgaggcc accggcctcg
     9481 acctcgaacg cgaccgcctg ctcgcggtcg gcgccatcgc cgtcgacggc ggcctgctgc
     9541 acccgcagga cgcctatcac gcgcagctcg agcccttgcc cggcgatgcg ctcgccgggc
     9601 tgctcggctt cgcccgcaac agcccgcttg tcgtgttcaa cgccaccttc aaccgcacga
     9661 tgctcgagcg cgcgttctcg agaatgctcg gcttcacgcc agagttcctc tggatcgacc
     9721 tctatttcct gctgccctcg ctgtttcccg agcggctgga acgccccggt cggctcgccg
     9781 actggatggc cgccttcgag atcgagacct tccagcgcca ccacgcgctc ggcgacgcct
     9841 gggcgatcgc gcaactcttc ctcgcggcgc agtcgcgcgc actctcgctc gcggtcacca
     9901 gtccgcgcgc gcttgccgag cacgcccagg cctaccggca aaagatcaag gtctgaaagc
     9961 aggccgacaa gaaccgggac gaatgcgtcc gacaggcgca gatttccctt tacaccggat
    10021 agctcgatgc gtatatttcc gcgaatgttt ttccggacat ccctacctgc cctgctcgcc
    10081 tccttcgcgc tcgcccttcc cgctcaaggc gcggagccgg ccccggcgca gcgcgagcac
    10141 gccttccagg cgtcttcggc gttcgaggag tacaccacgg ccgccgaaca actggtcgac
    10201 gaggcgctct cctacctggg tatccgctat cgcttcggag gcacttcgcc ggcgaccggc
    10261 ttcgattgca gcggactcgt gctcaacgtg ttccgtaatg ccgtcgggct cgacctgccg
    10321 cggagcgcga gcgagatggc gagcctgggc gacaagatcg gcaagcagga cctcaagccg
    10381 ggcgacctgg tgttcttcaa caccatgcga cgcaccttct cgcacgtcgg catctatctc
    10441 ggcgacggca agttcgtgca tgccccctcg agcggcggca aggttcgcgt cgagaacatc
    10501 tccgcgacgt actgggccaa acgcttcaac ggcgcccgcc ggctggtcga tgaggaatcg
    10561 ctcggtctca gcgcccaggt gatgcgcacg cactgagcgc cggcgaggaa cacgccgagg
    10621 aagaggcccc gcatgccggg gcctcttgct ttttcagcag gcagatgacg cccgccggcg
    10681 cccaccggat gccttctcgc agcgccgcgg gcattcgtcg cagcgcgaac ccatctgccg
    10741 atcgagcgcc tgctgcagcg cgcaggtgga gcgccggcag cacaccacct gcacgccctg
    10801 cttgcctgcg gcctcacgga agcgctgcat gacgttgtgg ccgagaaaat cggtgaacag
    10861 gatcaccagc ccgatcccgc tcggcaaggg cgtgctgcgg cgctggtgcg tcgtgtcgcg
    10921 gccgctgacg tgggcggcga tgcggatgcc gaagttgtgc agaacatccg ggatgttgcc
    10981 gaggcggtcg gcaccgacga gcagcgcatt catggaatct ccttgttgag aacatttctc
    11041 aacaataatg aagtgaatgc gatccatttg caactgcgaa cctttatcgt tagtatcgtc
    11101 gcaccgcaaa cacaccaagg ttcgcacacc atgagcaaga aagtcctcat cgcctcgatc
    11161 atcgccgcct tcggtctcac cggcaccgtc accgccgccg agctgaacgt ctactccgcc
    11221 cgccactacc agacagacga agagctctac ggcaatttca ccaagcagac cgggatcaag
    11281 atcaaccgca tcgaggcgaa ggaagacgaa ctgctcgagc gcatccgcaa cgagggcgcc
    11341 aacagtccgg ccgacatctt cgtcaccgtc gatgcctcgc gcctggcgaa ggccgacgaa
    11401 ctcggcatct tccggccggt gaagtccgcc gcgctcgagg cccgcatccc cgcccacctg
    11461 cgcgcaccga actggttttc ctactcgacc cgcgcacgcg tgatcgtcta caacccggac
    11521 atggtcaagg ccgagcaggt gcagacctac gagcagctcg ccgaccccgc gctcaagggc
    11581 caggtgtgca cccgctcggg cagccacccc tacaacctct cgctcggcgc cgccatgatc
    11641 aagcacaacg gcgcggaagc aaccgagaac tgggcccggg gcatcgtcgc caacttcgca
    11701 cgcgcgccca agggcggcga caccgaccag atccgcgccg tggccgcggg cgagtgcggc
    11761 gtggccatcg ccaacagcta ctacctcgcc cgcctgatga actcggacaa gcgcgaagac
    11821 caggccgtgg tcgcgaagat caaggcggtg tggccgaacc aggcgacctg gggcacccac
    11881 atcaacgtgt ccggcgccgg catgctcgag cacgcgccga acaaggaggc cgcggtcaag
    11941 ttcctcgagt atctcgcctc ggaccaggcg caggaatact tcgccaacgg caacaacgaa
    12001 tggccggcgg tgccgagcgt gaaggtggac aaccccgcgc tgaagaagct cggcgagttc
    12061 aaggccgaca ccctgccgat cggcgagctc gccgacacgg tggccgaggc ccagcgcatc
    12121 ttcgaccgcg ccggctaccg ctgatccgca gcccctgcgg cggccccctc gctgacgggc
    12181 tcgccgcagg gcgcaccggc actcgccggc gccagcacga cgcggttacg cccggcgtgc
    12241 ttggccgcgt acaaggcctc gtcggcgcgc gcataggcgg cctcgtagcc ctcgtcggcc
    12301 gcgtccagca tggtcgcgcc gatgctgacc gaatagtgca gcgcccccga gtcgacgctg
    12361 gccggcgaat ccgccaccac cctgcgcagc cggtccgccg cccgcaccgc gccgagcgcg
    12421 tcgatgctgg gaagcaggac cgcgaactcc tccccgccca ggcgtgcgaa cacgtcggcc
    12481 tcgcgcaact gcgcgctcac cgtcgcggtg aaatggcgca gcacctcgtc acccgcggca
    12541 tggccgtggg tgtcgttgat gcgcttgaag aagtcgaggt ccatcagcag cagcgctgcc
    12601 ggcgcctggt tgcggcgcac gcgtccgagt tcgccgcgca cgacctcgac gaagtggcgg
    12661 cgattccata gcccggtgag cggatcgctg gtggccagct gcagcaggct ctcctccatc
    12721 gccttgcgct gcgagatgtc ctggtggatg ccgaccatgc gcagcgcctc cccgcccgcg
    12781 ccgcgatcga gtacgcggcc cgccgcgagc acccagaccc aatgcccgct cttgtgcttg
    12841 agccggtatt ccgcgcgata ggcgtccttg gtgccgcgca ggtgcggctc gagcgagtcg
    12901 cggatgtcgt accagtcgtc cggatgcacc agggactccc agctcgatac attcgccggc
    12961 agctcatccg ctgcatagcc gagcatcctg caccacatcg ggctgaaatt cgctcgcccg
    13021 ctcggcacgt cccagtccca caaaccgagc tcggcacctt cgagtgcgag catcaggcgg
    13081 cgttcgcgct cggcgatctg ctcgcgcacc cggcgccgct cggtgaagtc ccggctgacg
    13141 cggatctcgc cgagctgcac gccgtcgagg tcgaaatagg gggtgcgcaa ggtgtcgagc
    13201 agacgtagcg ttccgtccgc cgcctccagc cactcctcgt ggttctctgc gcagctcacg
    13261 gtgtcctggc cggccccggc gagcggcagc cgccccgcga acacctcgcc gtcgctgcgc
    13321 ccgaccaggg ccgcctcgcg gatcccgtag gcctcggcga aggccttgtt gcagcccagg
    13381 taacggcctt cccgatcgcg gaaggcgatc aggtcgggca ccgaatcgat cagcccgcgc
    13441 agcagcgccc gctcggccgc cagcgcccgc tgtggcgcac gcacgccgga cacgaacacc
    13501 gcgcgataga tcatcgcgaa cgccgccacc ttgtaggcgt gcccgagcac gttgtaaagg
    13561 tcggcgaccg cggcataacg cgcgaaatgc agctcggcca gcgccatcgt cgccgcggcg
    13621 gcggccagcc agccagcggc ggattcgccg tccgccagcg cgcggcgcgc gagcaagatc
    13681 gcggcagcaa ggtgcagcag cacgagccca ccctccagca cgctctttaa cggggtgacg
    13741 ccctgcccct cgatgaaggt cgccggcaag acctgcgggt gcgcgaggac gagccagctc
    13801 cagccgaagg cgacgccgat cgcagcgagg acggcccagg ccgcggcggg tccgccgcca
    13861 tcgcgctccg gcagcacggc atgggcgagc aggcccagca caccgagcag acgcgccatc
    13921 agccagaagg cgatcgcctt gtccgcgctg ttgggcgtga agaagggcgg catgcccgga
    13981 aaggacatca ggtgggcgaa atcgagcagc cccaccgcga ggcaggctgc gccgagccag
    14041 accagccgtg ccccgcgcgc gctgcggcgc aggttccagc caagcgcgaa caccatcccg
    14101 gcgaccacga gcgagaagat ctccagccct gaatgcagcg ggaggtaatc gcgcgcatcg
    14161 gcgaacaggc gcaccggcgg catgaagacg ctgaggacga agagcacccc gagcaggccg
    14221 gtggcctgca gcggacgacg gaagcggtgc agcaccgagg tcgccaagat caacgcgcct
    14281 cgggcacacc gcaggagcgt ggtttgcccc cccagccgga aggcatggta tggacgaccc
    14341 gggcagcttg ccgagcgctg cacgggacga agattgaagg acgcggggac atcgcgtaat
    14401 tatgcatgat cgacccgcga ccgccgccgc ggcagcgcac cccgcgtgca ttatttcgcg
    14461 tcggcaggca cctcgctcag ccctcccggc ggcgcacgcg caggatctgg cgcgaaatcc
    14521 agaacatcgg cagcaagccc accgccacga tgatcagcgc cgcggtcgag gcttcggcca
    14581 ggcgctcgtc ggccgcaagc gtgtgcgcct gggtggcgag ggtgtcgaag ttgaagggtc
    14641 ggatcaccag cgtggcgggc agctccttca tgacatcgac gaacaccagc aggccggcgg
    14701 tgagcaggct accgcgcagc atcggcatgt gcacccgcct cagcgtcgcc cacttgccga
    14761 agcccaggct gcgcgaggcg tcgtccatgt tgggggtgat cttgcccagg ctggactcga
    14821 ccgtctgcag cgccactgcg aggaagcgcg ccaggtacgc gtagatcagc gcggcgatgc
    14881 cgccggtgag cagcagcccg gggttcgtcc cccacaacgc ctcccagccc tgggccagcc
    14941 agcgatccac ccgggtcagc gggatcagca cgcccaccgc gatcaccgag ccgggcaccg
    15001 cgtagcccag tccgacgatg cggttgagca cctgcggcag gcgcgagcgc gccaggcgtg
    15061 cggcgtaggc gagcagcact gcgagcaaca ccgccaccac cgcggtgatc gccgcgagca
    15121 cgaagctgtt gtgcgccagc tcgacgaagc gcgcgccgaa ctgggcatcg ccctcgagga
    15181 aggccatgcg cagcagcagc gccgccggca gcaggaagcc gagcagcagc ggcatgaagc
    15241 aggcgaacgc ggcgagcaca ccgagcgcca gcggcaggcg cagccgcacc ggcatcgcca
    15301 cctggcgcga ggtgttgttg aagcgcgccc gcccgcgcga catgcgctcc agggtcagca
    15361 ccaggaccac gaaggcgagc agcgcggccg acagctgcgc ggcggcgatg cggtcgccga
    15421 gcgagaacca ggcgcgatag atgccggtgg tgaaggtctg cacgccgaag taggacacgg
    15481 tgccgaagtc ggccagcgtc tccatcagcg ccagcgcggt gccggccacc accgccgggc
    15541 gcgccagcgg cagggagacg cggaagaacg agccccacgg acccagcccg agcgtgcgcc
    15601 cggcctcgag catcccgccc gcacgctcca ggaaggcggt gcgcgcgatc atgtagacat
    15661 acgggtagag cacgaacatg aacatcgcca ccgcgccgcc gacggtgcgc acgtcgggga
    15721 accagtagtc gccgcgcccc catccgaagg tctcgcgcag ccagccctgc accgggccga
    15781 cgaactggag aaaatcggta tacacgtagg ccatcacata ggccggcatc gccagcggca
    15841 gcaccagcgc ccactccagg aagcgccgcc cgggaaagtc cagcatcgtc gtcagccagg
    15901 ccgaggtcac gccgatcgac gacaccccca ggccgacgcc gatgcatagc accacggtgt
    15961 tgaacacgaa ctcgccgagc accgtgtcgg ccagatgcga ccaggtcgcc ccggtgtcgc
    16021 cggcgagcac gttggagaac accgacagca ccggcgccgc gatcagcgtg gccaccacga
    16081 ccgccagcac tgtcagcgaa gaaaaccgaa tgcgcatcgc gccctgccct gccgtcgaat
    16141 ccatgccgtt cccatgattg cgccccggcg ccggaaatcg gcccccgcga actgagcgaa
    16201 ttataattga tcgcatttgg aacaccgggg tgcaataatt ccccacgccc ataggccctc
    16261 gacgaccgcg atgtcccacc tcgacctttc ccgcatcgac ctcgccttcg gcagccacct
    16321 cgtggtccgc cagctctcac tgcagctcga gaagggccac atcggctgcc tgctcgggcc
    16381 ttccggctgc ggcaagacca cggtgctgcg ctgcatcgcc ggcttcgaac ggctggcagc
    16441 cggcgagatc cgcctcgacg gcgaggtcgt cagcacgccg caccacatgc tgccgcccga
    16501 gcgccggcgc atcggcatgg tgttccagga ctatgcgctc ttcccgcatc tctcggtggc
    16561 ggacaatatc ggcttcggcc tccggcgcga cagcgccgcc gtgcgcaacg cgcgggtgga
    16621 cgagctgctc gcgctggtcg gcctcgccgg acagggccgc aagtatccgc acgagatgtc
    16681 cggcggccag cagcagcgcg tcgcgctcgc gcgtgcactc gctcccaagc ccagcctgct
    16741 gctgctcgac gagcccttct ccaatctcga cgtcgaattg cgcgaacgcc tgtcgtacga
    16801 ggtgcgcgag atcatcaagg ccgccgacac caccgcgatc ctggtcaccc acgaccagca
    16861 cgaggccttc gcagtggccg acgagatcgg catcatgcac gagggtcgca tccagcaatg
    16921 ggatacgccc tacaacctct accaccgccc ggccaaccgc ttcgtcgccg acttcatcgg
    16981 ccagggtgcc ttcctgcgcg gcaaggtgct cgacgatcac cgcgtgcaga tcgagctcgg
    17041 cgtgctcgac agcacgcagc cgctggtgtg ctgcgccgac tgcgccggtt gcgtgcatgg
    17101 cagcccggtc gacgtgctgc tgcgccccga cgacctcgtg cacgacgacg ccagcgcgct
    17161 gcgcgccgag gtggtgcata aggctttccg cggcgcagac atcctgtaca ccctgcgcct
    17221 ggcgggcggc gcgcgcgtgc tctcgctggt gcccagccac cacaaccacg ccatcggcga
    17281 gcgcatcggc atccgcctcg acgtcgacca cgtcgtcacc ttctccgacg agcgcgccga
    17341 cgagtgcgcg cccgcccatc ccgcagcgtc tctcgcatga tcccctggct gggcgcggag
    17401 ccgggcttcc ccgccgacga gcgcgctttg cgcgaaccca acgggctgct cgccgccggt
    17461 ggcgcgctca ccccaccctg gctgctcgcc gcctaccgcc gcggcatctt cccctggttc
    17521 agcgagggcg aacccatcct gtggtggaca cccgaccctc gcctggtgct cgtgcccgac
    17581 gaattccaca tcagccattc gctgcgcaag accctgcgcc gacggcgctt cgaggtcagg
    17641 acggacaccg ccttcgccga ggtgctgctg gcctgcgccg cggcgcgcgc cggcggcccg
    17701 ggcacctgga tcacccccgc gatgcgcgcc gcctaccagc gcatgcacga actgggctgg
    17761 gcgcacagtg tcgaatgctg gcgagagggc cggctggtgg gcggactcta cggcatggcg
    17821 atcggccgcg tgttcttcgg cgagtcgatg ttcgcgcgcg agaccgacgc ctccaaggtg
    17881 gcgctcgccc atctcgcgcg gctgctcgcc gaacgcggct atggcatgat cgactgccag
    17941 atgaccaccg cccacctgtg ctcgctcggt gcacgcgagg tgccgagggc gcgtttcacc
    18001 gccgccctgc gccagtgggc gcacgacggc ccggcgccgc agcgctgggc cgagcgaaca
    18061 atgcaggact tcgactggaa ctgacgcagg acggcaagtt gcacgacgga cgcgatgaaa
    18121 tcggactatc cctacgccct catccagttc tacgccacgg cgccctacgc atgctcctac
    18181 ctggccgggc ggagcgcgcg ctcgcaggtc gccaccccgg gccatctcat cgacccgcag
    18241 gtctatagcg aactcgtgcg ccgcggcttt cgccgcagcg gggtgttcac ctaccgcccc
    18301 tattgcgacc actgccaggc ctgcgtgccg gtgcgcgtgc cggtcgagcg cttccagccc
    18361 gaccgcagcc agcgcaggac atgggctcga catgcagatc tgatcccgat cgagcgtccc
    18421 ctcgaattcg tcgaggagca ctaccagctc taccagcgct accagaccgc ccgccacgcc
    18481 ggcggcggca tggacctcga caaccgcgag cagtacgccc atttcctgct gcagagccat
    18541 gtcgacaccc gcctggtgga gttccgcagc tatggcagcc tgcgcatggt gagcgtgatc
    18601 gatcgcctca ccgacggcct gtcgagcgtc tacaccttct acgatccaga ccagccgcgc
    18661 agcgccttcg gcacttgggg cgtgctctgg cagatcgagg cgtgcagggt gctcggactg
    18721 ccgcacgtgt acctgggcta ctggatcggc gagagcccga agatggccta caaggcgcgc
    18781 ttccgcccgc tcgaggcgct gatcgacggc gactggcagc cctttccggg cgatttcgcc
    18841 tgatcacggc gcccggcggt cgggtcgact cgctacaatc tcgcgatgct ctacgaactc
    18901 gcccgccccc tcctgttcgc gctcgacccc gagaccgccc acaacctcac cctgcatggc
    18961 ctgcactatg ccggcaagct gctgcccgcc gccgagccgg agccggcgag cgcggtcgag
    19021 gtcatgggcc tgcgctttcc caaccgcgtc ggcctcgccg ccgggcttga caagaacggc
    19081 gaggcgatcg acggcctcgc ccgcctcggt ttcggcttcc tcgagatcgg cacgatcacg
    19141 ccgcgcccgc agccgggcaa cccccgcccg cgcatgttcc gcctgcccga ggtgcagggc
    19201 atcatcaatc gcatgggctt caacaaccac ggcgtcgacg tcctgctcgc gcacgtgcgt
    19261 gcggcgaagt accgcggcat cctcggcatc aacatcggca agaacttcga caccccgatc
    19321 gagcgcgccg ccgacgacta cctcgcctgc ctggaaaaag tgtacgccct ggcgagctac
    19381 gtcacggtga acatctcctc gcccaacacc aagaacctgc gccagctgca gggcgaatcc
    19441 gaactcgacg acctcctcgg ccgcctcaag gcggcgcaga cccgcctcac cgagcaacat
    19501 gggcgctacg tcccgctcac cctcaagatc gcccccgacc tcgacgacgc gcaggtgagc
    19561 aacatcgccg acgcgctgcg ccgccaccgc atcgacggcg tgatcgccac caataccacg
    19621 atcgcgcgcg acaaggtgca gggcatcgcc cacggcaacg aacagggcgg cctgtcgggc
    19681 gcgccggtgt tcgaggcttc caccgccgtg gtgcgcaagc tcgcgcacgc gctcgccggc
    19741 gagttgccga tcatcgccgc cggaggcgtg ctcgagggcg cacaggcgcg cgccaagctc
    19801 gaggccggcg ccgctttggt tcagctctac agcggactca tctaccgcgg cccggcactg
    19861 gtgcgcgaat gcgtgcgcgc caccgcgggg tgagtccttc cccgacctct tccttcaccc
    19921 ggcgagcagc acgccgcgct cgtccgcagc ggcgcccgtg ccgaccttcc gaccctccga
    19981 ggagcccaag atgactcatc gccgccagtt cgtcctctcc gccgccgcgc ttgcggtcgc
    20041 agccaccctg cccttcgccg cccacgccgc ggacgccgcg aaggtcggct tcgtctatgt
    20101 gagcccgatc ggcgatgccg gctggaccta ccagcacgac ctcggtcgca aggagctcga
    20161 gaagacgctc ggcggcagga tcgagacgcg ctacgtcgag aacgtcgcgg aaggtgccga
    20221 tgccgagcgc gtgatccgcg agttcgccgc cagcggcagc aagatcgtgt tcgccaccag
    20281 cttcggctac atgaactacg tcgagcgcgt cgcccgccag ttccccaacg tcaccttcct
    20341 gcacgccacc ggctacaagt cggcgaagaa cttcgcgccc tacaacgcgc gcttctacga
    20401 aggccgctac ctcaacggcg tgatcgccgg caagatgacc aagtccaacg tcctcggcta
    20461 cgtcggcgcc ttcccgatcc ccgaggtgct gcagggcatc aacgcgttca ccctgggcgc
    20521 gcgcagcgtg aacccgaacg tcgaggtgcg ggtgatctgg gccaacagct ggtacgaccc
    20581 gggcaaggag cgcgaggcgg cgatgaccct gatctcgcag ggcgccgaca tgctcaccca
    20641 ccacaccgac tccaccgcca ccgtccaggc cgcggaagaa aagggcgtgt acgccttcgg
    20701 ctaccactcc gacatgtcga agtacgcgcc caagtcccag ctcaccgcca ccacccacca
    20761 ctggggcgag ttctacgccc gcaccgtcga cgcggtgctc gggggcacgt ggaagccgga
    20821 gggcgtgtgg ggtggcatga aggacggcat gatccgcctc gccccgctca acccggcgat
    20881 tccggccgac gtgcaggcca tggtcaagga actccagggc cgcatcaccg ccggcagcct
    20941 gcatcccttc accggcccgg tgctcgacca ggccgggaag gaacgcctcg ccgccggtgc
    21001 ggtgatggac gatgccaccc tgggcaagat ggactacttc gtccagggcg tcggttcgcg
    21061 cctgcccacc gccaagtaag cctgcttccc caccacgtcg gttccatccg gcgacgagac
    21121 cgccctcgtg cggagcgggc agccccccgc tcgcttcacg gcgtgggcgg gtcggcgcga
    21181 tccgggcggt ccgcccacgc ctcgtcgtca gccggaaggc cgttgcgcag gcaggccgag
    21241 atcccggtgg cgatcgcgtc ggccatgcgc gcctggcgtt cggggtcgcg cagcaacagt
    21301 tcctcgtcgc ggtttttcag gatgccggct tcgaacagca ctgccggcat gcgcgcgtgg
    21361 cgcagcaccg ccagcccgtc gtagtggtgc acggcgtgct cgcggtcagc gtagctgcgc
    21421 cggcggccgt tcttgtgcgt gggctcgaaa cccatgcgct gcaggcgggc gccgatcgca
    21481 cgcgcacaca ggatgctcat ggccgtatcg gggttgtcgc gcgagacgaa gagcgaatgc
    21541 ccggcgaact cgtcgttgta gtcgagcgca cgcccctccc aggtccacgg gcgcaactcg
    21601 acggcattga ccgagtcgtg atgcagcgag agcaggaagg ccgctccggc ctcgccggcg
    21661 gcctgcggcc gcgcgcgcag gctcgcgatg cggccgtcgg cgttgatggt gctcaccgcc
    21721 cagccgcgcg cgtgcagtgc atcgaccaca tgcacggaaa gatcgcggtt gaactcgaac
    21781 tcgctgcggc cgcgcgcgct cgtcgccccg ggcgcgtcca gggtgtggcc gacgtcgaca
    21841 gcgaccggcc cgggcccctc gccgccagcc agcgcccccc ccgcgatcgc cgccagcacg
    21901 agcgcaagca gcccgtggcg cggcgcacgc cgccacgcat ccccggtccg tgcgtgcccc
    21961 gaggcctgca aagacccgca aagcgagcgc ccatgcggat ggaaaagttc cactcgggct
    22021 acatcttgat tcaaatcatg gagagcgcgg gtgcccgccg tgacaatccg cccaccatcg
    22081 gcattgccgt ggacagttgt acccctgacc cacgcgggcg aaccgagacc ttcctgacaa
    22141 aacgagacaa gcgatgagga gtactccatg agcggtattt tcacaaaatc gatggcgcgg
    22201 aacatcttct acggggggac gacgttcttc ttcctgttgt tcctggcgct caccttcgac
    22261 accgagcgcg agctgcccaa ccgggacaac cgcgccgcca tcacccccga ggttgccgcc
    22321 ggcaagcaca tctgggaaac ccgcaactgt ctgggctgcc acacgctgct cggcgagggc
    22381 gcctacttcg ctcccgaact cggcaacgtc tacaagcgcc gcggcggcga cttcatcaag
    22441 ggctggatgc aggccatgcc caccggagcc cccggccgtc gccagatgcc gcagttcaac
    22501 ctcaccgatg aagagctcga ccagctggtg gccttcctga agtacacctc ggaaatcaac
    22561 accgccaact ggccgcccaa catcgagggc tgatcggaac tctggagacc acaaggggat
    22621 atccatgcaa tacaaatcac aagcggtcgc catgccctac ttcatcgcgg cgatcggtct
    22681 gttcgtgggg caaatcatct tcggcctgat tctgggcctg cagtacgtcg tcggggactt
    22741 cctgttcccg gagatcccgt tcaacgtcgc gcggatggtt cacaccaacc tgctgatcgt
    22801 gtggctgctg ttcggcttca tgggtgcggc ctactacctg atcccggaag agaccgagac
    22861 cgaactcttc agcccgaagc tggcgctggc gatgttctgg atcttcctgg tggccggtgc
    22921 gctgaccatc gtgggctacc tgctcgtgcc ttacagcacg ctggcgtcca tgacgggcaa
    22981 cgacatcctc gccaccatgg gccgggagtt cctcgagcag ccgctgttga ccaagatcgg
    23041 catcgtgatc gtcgcgctcg ccttcctgtt caacatcacg atgaccgtgc tcaagggccg
    23101 caagaccgtc atcagcatcg tgctgctgct cggcttgtgg ggtctggcga tcttcttcct
    23161 gttctccttc tacaacccgg tcaacctcgt ccgcgacaag ttctactggt ggtgggtggt
    23221 tcacctctgg gtggaaggcg tgtgggaact gatcatggcc gcgatgctcg ccttcgtgct
    23281 catcaaggtc accggtgtag accgtgaagt gatcgagaag tggctgtacg tcatcatcac
    23341 cctggcgctt gtgaccggca tcatcggtac cggccaccac tacttctgga tcggcacgcc
    23401 ggaatactgg cagtggtggg gttcgatctt ctccgcgctg gagccgatcc ccttcttcgc
    23461 catgaccgtc ttcgcgttca acatggtcaa ccgtcgtcgt cgcgagcacc cgaacaaggc
    23521 tgccgtgctg tgggcgctcg gtaccggcgt gatggccttc ctcggcgctg gcgtgtgggg
    23581 cttcctgcac accctggctc cggtgaacta cttcacccac ggctcgcaga tcaccgccgc
    23641 gcacggtcac atggcgttct acggcgccta cgtgatggtc gtgatcacca tgatcagcta
    23701 tgccatgccg atcctgcgcg gccgcgccgc caactcgacc aaggcgcaag tgctggagat
    23761 gtgggcgttc tggctgatga ccatcgcgat ggtcttcatc accctgttcc tgaccgccgc
    23821 cggcatcctg caggtctggc tgcagcgcgt gtccgacacc ccgatgtcct tcatggccac
    23881 ccaggatcag ctgatgctgt tctactggat gcgcgaatgg gcgggcgtga tgttcttcat
    23941 cggcctgctg gcctatctcg ccagcttctt cgtgaagggc gatgccaagg gggtcgtgca
    24001 cggctgagcc ctgccgaccg aaatgcggta ataagcctgg atcatccagg gtaaggggtt
    24061 ggggcggcgc gagccgcccc tctttttttg cgtgccacgc tcgcagcatg gagcggccgc
    24121 tagaatgtcg ggcttcgtcc gcgcccgaac ggcactccgc tgccaccgga tccccccggc
    24181 gcgcgcgtcc catcgcctcc gctcgctccc ctccgcccgt gcccaacgcc tcccttccgc
    24241 cctccccgtc gtcccacgac gcacctcccg tcctgcaccc gagcggcgtc gagcgcccct
    24301 tccgcatcgc gatcaacggc tacggccgca tcggtcgttg ctacctgcgt gccctgcacg
    24361 aagccggcct cgccgagcgc taccgcatcg tcgcgatcaa cgagcctgcg gacctcgcca
    24421 gcatcgccta cctcacccgc ttcgactcga cccatggcag gtttccgggg gtggtggagc
    24481 gcagcgagcg cgagctgatc gtggacggtc atgcgatcca cgtgagccac gcgagcaccc
    24541 cggaagaggt cgactggacc gtgctggggc tcgatctcgt cgtcgagtgc tccggacagt
    24601 acggcggacg ccgcgacttg cagcgcttca tcgctgccgg ctgcccgcga ctcctgctgt
    24661 cgcaccccgg caacggcgcc gaggatgtgg atgtcacagt ggtgttcggc atcaacgagg
    24721 acgagctgca cggccccgag cgcctggtgt ctaacgcgtc gtgcaccacc aacgccgtgg
    24781 ttccggtgct cgactgcctg caccgcgaga tcggcatcga gcaggccctg ctcaccacac
    24841 tgcattcggc gatgaacgac cagccgctga tcgacggcta ccaccacgcc gacctgcgcc
    24901 gcacgcgctc ggcgatgcag tcgatcatcc cggtgtcgac cggcctggcg cgcggcgtcg
    24961 aacgcctgct cccggcgctg gccggccgcg tgcaggcgaa ggcgatccgc gtgcccaccc
    25021 acaacgtctc cgcgatcgac atggtgctga gcttcggccg cgaggtctcg gccgaggccg
    25081 tcaaccgcct gctgcgcgag accgccgccg ggccgcagcg tggcctgctc gcgtgctccg
    25141 acgaggcgca cgcctcgatc gacttcaacc acgacccgca ctcggcgatc atcgactgca
    25201 gccagacgcg cgcagtcgga gcgaagatgc tcaacctggt ggtgtggttc gacaacgaat
    25261 ggggcttcgc cagccgcatg gtcgacgtca cccggcgctg gctggcggcg gcgggctgaa
    25321 cacgaacgaa tcccaattaa ccacgatcaa gttaaacccg cgcccttctg cctagactgc
    25381 gccgcatgaa taccgtcacc actccgccga gttccctgcc ggccggcgga ccggcacagg
    25441 cgcacggccc ccgccttgcg ggcgacctcg cgatctggtt cttcatcctc gccgagctgc
    25501 tcgccttcgg ggtcttcttc gcggcctacg ccttcgcgcg cgccaagaac atcgagctct
    25561 tcgccgccga gcaggccgcg ctcaaccgca acgcgggcgc gctcaacacc gtgctgctgc
    25621 tcactgcgag ctacttcgtg gtgcgcgccg tgcaggccgc cgaggcccag gcctcgcgac
    25681 agtgcgccaa ctggctgggc ggcgcgatcc tcaccggcct cggcttcatc gtcgtcaagc
    25741 tgagcgagta cgccgcagcg ttcgagcaca acatcagcct ctcgagcagc accttccaca
    25801 tgttctacct gtcgctgacc ttcttccact tcatgcatgt gatcctgggt ctggtaatcc
    25861 tgatcgcgat gtggaacggc gcgcgccagg gccgctaccg cccgggcgag atgaacggac
    25921 tggagaccgg cgccgcctac tggcacatgg tcgacctggt ctggctgatc ctcttcccgc
    25981 tcgtctacgt catccactga ggagcccgcc atgagcgcac gcatgcacgg aacgccccgc
    26041 ggctccacga tcctgttcgc caccctgatc ctcgccaccg tcgccacctg gggcatgggc
    26101 tcgctcggcg tgaccggcac ctggggcgtc gccatcctcg ccgcgatctc gttctggaag
    26161 ggcgcggtgg tcatcctcga tttcatggcg ctgcgccatg caccgctgct ctggcgcgcg
    26221 atcacgatgg gctggatcat cgtcgtctgg gcgctgatcg ccatcgccta catcaaggga
    26281 ctcgcccaat gaagcccgat acgcccaacc tcgacattcc cttctaccaa cccgccggcg
    26341 acgaggtgga catcttccag cacgcctgga agaaccgcct gcccctgctg atcaagggcc
    26401 ccaccggctg cggcaagacg cgcttcgtct cgcacatggc cgcacgcctc ggcctgcccc
    26461 tgtacaccgt ggcctgccac gacgacctca ccgccgccga cctggtcggc cgccacctga
    26521 tcggcgacgg caagacgatg tggtgcgacg gcccgctcac gcgcgcggta cgcgaaggcg
    26581 gcatctgcta cctcgacgag gtggtcgagg cacgcaagga caccaccgtg gtgctgcacc
    26641 cgctcgccga cgaccgtcgc gtgctgccga tcgaccgcac cggcgagctc ctcgaggcgc
    26701 caccctcctt catgctggtg gtgtcctaca accccggcta ccagaacctg ctcaagggca
    26761 tgaagccttc gacccgccag cgcttcgtca gcctgcgctt cgacttcccg cgccccgagc
    26821 aggaagaggc ggtgctgatc ggcgagaccg cctgcgcacc ggacctggcc aagcgcctgg
    26881 ttgccatcgc caacgcgctg cgcgcgctca aggaccgcga cctggaagag gccgcgagca
    26941 cccgcctgct cgtgtatacc gccctgctcg tcaaggacgg catggacccg gtcgccgcca
    27001 gccgggtcgg catcgtcgag agcctgaccg acgacgccga cgtcgccgag ggactgatgg
    27061 aggtggtcgc cgccaccttc ggccgctgac ggccgggaca tcgagatgga aatcaagcgc
    27121 gccgatccgc tccgccacgt catgcccggc ccgcgcctgg cggtcgtcgc cgaggcgctg
    27181 ttcctgatca acctgatgct cctgcccggg ctggcattcc tcgcgctgat ggtgctgtgg
    27241 tacctgcatc gcgggcaccc cgatccgctg gtgcgcaatc acctgcgcca gaccgggtgg
    27301 acctgcatct ggggcgggct gctgctggtc gtgatcagcg tcgcgatctt cctgctcggc
    27361 ggcttcgaca atccgtggac ctgggtgatc ggggtgctct atttcacctt cgtccactcg
    27421 agcctgatcc tgctcggcgt catgggcctg tcgcgcgcga tcaacggccg cagctggcgc
    27481 ttcccggtgt tcggaccgcg cgagccctcc tgagccggcc ggtgaccacg atgaacaccg
    27541 gagcccctgc gcgcaaaccg cagcgcgtca tcccgatcgc caccgcccct gcgggcgatg
    27601 cggccgccgc cgcggcgatg cagcgccgcc ggcgtgcgtt ccaggccggc ttcttcgcgc
    27661 tcttcgtgct cgcgccggta ttcgatctct tccgctacga cctcgacgcc gaccacgcct
    27721 ggctgctcgg catggaatgg cggctcggcc tcgacccctt cctcgccggc caggcgagcg
    27781 cactcgaggc ggccgggagc atcatcctga atctcttcgt gcccctgctc gcgctcgccg
    27841 cgctggtcat cggcgcggcg tggaagtggg gacggctgta ctgcggctgg ctgtgcccgc
    27901 acttctcggt ggtggagtcg atcaaccggc tgatggcgcg cgcttcgggc aaggcctcgg
    27961 tatgggaccg ccacctgctg ccggcgtgga agcccgacgg ccgccccgcg cgggtggacg
    28021 cgcgctggtg gggcgtcgtc gtgccggcgg cggtgggctt cgccttcctg tgggcggtgg
    28081 tgctgctcac ctacctgctg ccgcccgcgc aggtgtacgg caacctcttc gccggcgagc
    28141 tcacgcgcaa ccaggcgatc ttcctcaccg cggccacggt ggtgctgagc ctcgagttcc
    28201 tcttcgcacg tcacctgttc tgccgcttcg tgtgcgccgt cgggctgttc cagagcctgg
    28261 cctggatggg caaccgcgac gcgctggtgg tcggcttcga gcgcgagcgc gcgagcgatt
    28321 gctcgaactg cctgcccgac cgccagtccg cctgcgacgc cgcctgcccg atgcggctgc
    28381 gcccgcgcaa catcaagcgc cacatgttca cctgcaccca gtgccgccaa tgcgtcgacg
    28441 cctgcgcgac cacccagcac gccaacccgc aggggccgct gctgtcctgg gtggccggcg
    28501 aggcggcgcg ccagaacgag gccgggttcc gcgcactcaa ggatcgctga cgccatggaa
    28561 gaaaccgtag gcctgctctg gcaccgcctc atcacccgcg ccgccggcgg acacttcccc
    28621 aaggcggcgg tccgcctgcg cgacatcgag aagaccgcgg gcgtgttctt ccgcgccctc
    28681 ggcggcgacc ccggcctgcg cctggcggcc gccaccaccg acgagcatgg cgcgcggcgc
    28741 agcctgctgc agcgcatcgc cggcgccgac gaacgcgtcg cgcgcgcgcg catggacgtg
    28801 tccacgctgc gcctgccacc cgagctcgac gtgctgcccg agcgcggcct caaccgcgac
    28861 ctctacctct ggctcgccgc cgtggccgcc gcgccccccg cggaggatgg cgacggccac
    28921 cccgcgctcg gccccgtgcc cgacgagccg atcgacgagg ccgccacccg cgagctgcgc
    28981 gagaaccagg ccgccaccct gcgcgccctc gcacgctggc ccgggatcga ggcgcgctat
    29041 cgccgcctcg tcgacgcggt gatcgcccag cgccccaggc tcgacaagct gccgcccgcc
    29101 gaggccgaac gcgagaagct gatccgcctc gccctgcacg cccccggcag cgtcgccgcc
    29161 ctgccccgcc tgcccgccaa ggcgcgcgcc acccgcctgc aagcggagct gcgtcgctcc
    29221 gcaggtcgac ctcgggcagc acgcgctgct gcatcagcca gcggttggtg tcgtgatacg
    29281 cctcctcggt gaagtgcgcg aactcgtcgt gcagcacgtc ctgcagcagg cgccaggttt
    29341 cctggctcag cgccgcgctc tgccacgccg acgcgacgat gcgagcgagc acgtgcggcc
    29401 gcagcacgtc gttggtgtcg agttcgtcgc gccgctcgag ccagatcatc cgcgagcgca
    29461 ggtcggtgaa ctcccacgaa gcgcggtcca tgatcgcgag cgcgaggcgc gaactgagga
    29521 tctcggcctc tatcgtgtcg ttgtcgacga gcgtcatggc cgcctatccg agcaagccgg
    29581 cgggaagctg cgtgcc
//
LOCUS       DAOQNN010000005        26929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_535_length_26929_cov_10.347920, whole genome shotgun sequence.
ACCESSION   DAOQNN010000005 DAOQNN010000000
VERSION     DAOQNN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 26929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26929
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_535_length_26929_cov_10.347920"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /gene="carB"
                     /locus_tag="PKV97_00915"
     CDS             complement(<1..1727)
                     /gene="carB"
                     /locus_tag="PKV97_00915"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNB04463.1"
                     /translation="MPKRTDIKTILIIGAGPIIIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPETADVTYIEPITWQVVERIIDKERPDAILPTMGGQTALNCALDL
                     GRHGVLAKYGVELIGASEEAIDKAEDRLKFKDAMTRIGLGSARSGIAHSMEEALQVQG
                     GIGFPVIIRPSFTLGGTGGGIAYNMEEFQEICKRGLEASPTNELLIEESLLGWKEYEM
                     EVVRDRADNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQIMRNASIAVLREIG
                     VDTGGSNVQFAINPQDGRMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LRNDITGGATPASFEPSIDYVVTKIPRFAFEKFPQANDRLTTQMKSVGEVMAMGRSFQ
                     ESFQKALRGLEVGVYGLDEVEADREDLEHEIASPGAQRIWYVGQAFREGMSLEQVFNL
                     SKIDPWFLAQIEDIVLSEKALAGRSLKALQAAELRELKKKGFSDRRLAKLLNADETAV
                     RLHRHTLGVRPVFKRVDTCAAEFATSTAYLYSSYEDECEAMPTERKKIMVLGGGPNRI
                     GQGIEFDYC"
     gene            complement(1813..2955)
                     /gene="carA"
                     /locus_tag="PKV97_00920"
     CDS             complement(1813..2955)
                     /gene="carA"
                     /locus_tag="PKV97_00920"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNB04464.1"
                     /translation="MSAFPPALLALADGTVFHGKGIGAVGSTVGEVVFNTSMSGYQEI
                     LTDPSYCRQIVTLTYPHIGNTGINPEDIESSRVQAAGLVIRDLPILPSNFRMTQRLDA
                     YLRAEKVVAIAGLDTRKLTRVLREKGAQAGCIVTAAAGQALDAEAAVAQARAFPGLAG
                     MDLAKVVSVAERYEWKQAEWALGSGYSDRADGRFHVVAYDFGVKYNILRMLVERGCRL
                     TVVPAQTPASEVLALNPDGVFLSNGPGDPEPCDYAIAAIRELVAARMPTFGICLGHQL
                     LALASGAKTMKMPTGHHGANHPVKDLDTGKVLITSQNHGFAVDPATMPANLKVTHVSL
                     FDGTNQGIARTDVPAFSFQGHPEASPGPHDVAYLFDRFIGLMEAAK"
     gene            complement(3116..5617)
                     /locus_tag="PKV97_00925"
     CDS             complement(3116..5617)
                     /locus_tag="PKV97_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB04465.1"
                     /translation="MSGAAESRGSPPDPGSVEAELSRLRAERDAALRSLARARRHLDL
                     VSANAGAGVWYMDLRGGSIRLDERWRRLLGYDGGDAGDWMALVDEEGRAALQQALRAH
                     LGGEAAELRAEIRIRAQDASWHWIEVCGNASGRGEDGRWARVEGTYRDVTERKLREIE
                     LLQAKEAAEAASRAKGDFLANMSHEIRTPMNGIIGMTDLLLDSTIAGEQREYLQTVKS
                     SAEALLTIINDILDFSRIEAGRLSVEQIDFSLHSVVSETCRALALRASQKGLELYYDV
                     APDVPSVLRGDPTRLRQILVNLIGNAIKFTEHGEVEVRVRCVQRNGGAASIEFLVRDT
                     GCGIAPDKLEAIFGAFSQADNSTTRKYGGTGLGLAICRQLTELMGGRIGVDSVPGAGS
                     RFEVTLPLRAVADPTPLRVGALAGTRVLVASANAAFADFLCATLQEAALRAEAACSGV
                     AVEQALARAADGSDPFAFLLMDADLPEPGGFALAQRFAAEWPRPDRIIPMMASHSQRN
                     DALRCEELGLGYRLAKPFSAEDLLAVLRMAQGGEREAAEPPPFELAPVELRSTVGMEP
                     PARVLEVLVVEDNPVNQTVAKRMLEHVGHSVTVANNGEEALEAIDNRHFDVVLMDVQM
                     PVMGGIEATQAIRAREARRSWVLAGDWKPVPIIAMTAHAMEGDRERCLQAGMDDYVAK
                     PVRPEALFAAIERVLGPADADEVAGDITLLELDPADRRRIACLEDARAMFAGEDGVVE
                     QLVEFFLRDLPRTVADLHKGAADMDFARLRELGHMIKGSVGVFSAQRAVDAARRLEEL
                     AAAGDPRALTSQAGLAAREIELLGEVLREETACGR"
     gene            complement(5626..6432)
                     /gene="dapB"
                     /locus_tag="PKV97_00930"
     CDS             complement(5626..6432)
                     /gene="dapB"
                     /locus_tag="PKV97_00930"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNB04466.1"
                     /translation="MSQIRVAIAGASGRMGRMLIEAALKEEGVVLAAAFDRSDTPFIG
                     RDAGEMAGVATGVLITDDAAAAIGAADCVIDFTRPEGTLAHLALARAAGKAMVIGTTG
                     FDAAGKTAIAAAAEHVPVVFAPNMAVGVNAVFRLLEVAARILSEGYDVEVIEAHHRFK
                     VDAPSGTALRMGEVVARELGRDLETCAVYGREGHTGERSAETIGFSTIRGGDVVGDHT
                     VLFAGIGERIEITHKSGSRMPYALGSMRAARFLAGRAKGLYDMQDVLGLR"
     gene            complement(6499..6906)
                     /gene="bamE"
                     /locus_tag="PKV97_00935"
     CDS             complement(6499..6906)
                     /gene="bamE"
                     /locus_tag="PKV97_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNB04467.1"
                     /translation="MRSLLAALIVSSGLLAGCSFDSVVGLVDPYRIDIRQGNYVDQEM
                     VAQLRRGMTRDQVRYVLGSPLVVDMFRKDRWDYVYRFRPGSGDAQQRVISLFFAGDVL
                     DRIEGDVAAASGAPAAAAQERSRVVEIPPASAR"
     gene            7156..7596
                     /gene="fur"
                     /locus_tag="PKV97_00940"
     CDS             7156..7596
                     /gene="fur"
                     /locus_tag="PKV97_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HNB04468.1"
                     /translation="MSDNSRNLKSIGLKATYPRLKILDLFQSSEQRHLTAEDVYRTLM
                     NEGMDIGLATVYRVLTQFEQAGLLERHYFESGKAVFELAEGGHHDHLVCMQCGKVEEF
                     FDAEIERRQNQIALERGFAIREHALYIYADCQRENCPNLGREKD"
     gene            complement(7665..9320)
                     /gene="recN"
                     /locus_tag="PKV97_00945"
     CDS             complement(7665..9320)
                     /gene="recN"
                     /locus_tag="PKV97_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNB04469.1"
                     /translation="MLRRLTIRDFVIVDRLDLEFAAGFGALTGETGAGKSILLDALGL
                     ALGGRGEAAMVRSGRERADVAAEFDLPAGEGLAAWLVEQELPADEGMLILRRTIDAGG
                     RSRAWINGVAATLAQLRTAGEWLADIHGQHAHHALLRADAQRALLDAQAGAGALVAEV
                     AARHREWQRLLRLQREAEADSASSARERELLAWQLRELEQVGFDIEEWAEINAEHARL
                     AHAAGLIEGAEAAVVALGEGELAVCSVLGRLDGRIAALADFDAGLADVRELISAAAIQ
                     ADEALHALRRYRDRLDLDPGRLAELERRIAAVMEAARKYRVQPEELPALAQQWRERLD
                     TLEATADPARLAAAAQAARAAFESVAAELTAARLPAAHRLSEEIAAAMQDLAMAGGRF
                     EVALTPCAPSAAGVEEVEFRVAANAGQPLRSLAKVASGGELSRIGLAIQVITSRDSAV
                     PTLIFDEVDVGIGGRVAEIVGKLLARLGQDRQVLCVTHLPQVAACADWQWRIAKREQG
                     GETLSEVAPLDDAARVEEVARMLGGVNITATTREHAAEMLGRR"
     gene            complement(9403..10284)
                     /locus_tag="PKV97_00950"
     CDS             complement(9403..10284)
                     /locus_tag="PKV97_00950"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNB04470.1"
                     /translation="MSTPFRTLALIGKYQSPDVAESVLSIARFLRDRGLAVLIEQGTA
                     SSIGGAHDFPVASYEHIGASADLAVVIGGDGTMLHTARRLIEHGVPLVGVNLGRLGFL
                     TDIARSSATERLAEILDGAFTAEDRFMLDVEVLRGGARVFHTLALNDVVVNKGELGRM
                     IEFELSIDEEFVYTQRSDGMIVSTPTGSTAYALSANGPILHPSVGGIALVPLCPHALT
                     ARPITLPDSCRIDIVLLPPHDARVHFDGQTRFDLRAGDCVRMTRSSRSLRLLHPEGYS
                     YFAMLRQKLHWSAAPRV"
     gene            10404..11432
                     /gene="hrcA"
                     /locus_tag="PKV97_00955"
     CDS             10404..11432
                     /gene="hrcA"
                     /locus_tag="PKV97_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNB04471.1"
                     /translation="MNPLDARSQILLKTLIERHIADGQPVGSRALSRYSGLELSPATV
                     RNVMSDLEEMGFIASPHTSAGRIPTALGYRFFVDSLLTVQPLENARVRALEGQLLPNQ
                     PQRLISSASHLLSELTQFAGVVVAPRKDAVRIRQFEFISLAENRILLIIVTTGGDVQN
                     RLLITRRAYTPTELNGAAAYLNEHFAGLSFEEIRRRLGDELRQLRSDMSELMTATVDA
                     GSEAAEDNAASYVLSGETKLLDVEDLSSNMSQLRELFRLFEQRTGLLQLLDLSNRAEG
                     VQIFIGGESGLAPLDGCSVITAPYEVDGQVVGSLGVIGPTRMAYDRVIPIVDITARLL
                     SNALSSKH"
     gene            11484..12581
                     /gene="hemH"
                     /locus_tag="PKV97_00960"
     CDS             11484..12581
                     /gene="hemH"
                     /locus_tag="PKV97_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNB04472.1"
                     /translation="MPRYRPEPIHRHDSTPRTGVLLVNLGTPAAPTAAALRPYLKQFL
                     SDPRVVEIPRLVWWPILNGIILNTRPKASAQKYASIWTDEGSPLLVHTVRQTKLLAGY
                     LAKDGYDGLDIDYAMRYGAPAIGDTLDAMRARGCTRILVVPMYPQYSASTTATVVDEV
                     GSSLARWRNQPEMRFVRNFHDDPGYIDALVKSVREHWAANGEPERLLLSFHGVPRRSL
                     DLGDPYHCECHKTGRLVAEGLGVARERVLVTFQSRFGKAEWLKPYTQPTLEQLAAEGV
                     RRVDVMCPGFVADCLETLEEIALECKEAFLEKGGQAFHYIPCLNERDAWIKALAALVR
                     KHLGNWLEIAAPDAAARSAALERARTLGAGQ"
     gene            complement(12669..14033)
                     /locus_tag="PKV97_00965"
     CDS             complement(12669..14033)
                     /locus_tag="PKV97_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNB04473.1"
                     /translation="MNRIPERIGKYRIIKEIGRGATAVVYLAETPDYPEPVALKHVRF
                     DDKARDEAKWNRRLIKLLKAERVVSGRLDHPNIIRIHDASIEPDEAYVVMEYFPGGSL
                     ERYCSFQNLLPIHRTIGIVFKCCMALDHAYRQGIVHRDIKPANILVDEQDNVKITDFG
                     LALNINKKLDTDSTFIMGVGSPAYMSPEQIKGYPLNQKTDLYSLGVLLFHMLTGRLPF
                     RASNPAQLVYKIINADPPSVSQLNPDVPEQMDAVIRKALEKDLYSRYRNGAEFAKDLA
                     AVRYQILDEGHVHHDAERFARLRKLAFFTEFEDVDLWEVMRICAWRKVGSDLGLMREG
                     DLDQRFGIVLEGKVELSIGGRRVLEVGPGEVFGEQAWLDQLEHKQLLSAVSLDELSYL
                     EVNPAALALASEEVLERFRRQLGTVAVRRLAAVSRQAAEHAPAAVRAVRAAPVVHDLQ
                     IVDY"
     gene            14203..15522
                     /locus_tag="PKV97_00970"
     CDS             14203..15522
                     /locus_tag="PKV97_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HNB04474.1"
                     /translation="MSEAVDLLINARWIVPVEPRDAVLADHAVAIRGGRIVDLLGQDE
                     ARTRYVADEVVELPRHLLIPGLVNLHTHAAMSLLRGIADDLPLMRWLEEAIWPAESRH
                     VSAAFVRDGTLLAAAEMIRGGITTCSDMYFHPEAAAEAFAAAGMRAVVGAVVLEFPTS
                     YASDPEDYLRKGLAARDRWQGHPRLGFSIAPHAPYTVSDDSFHQVQTLADELGLPIHV
                     HIHETAQEIADSLAVHGCRPLARLARLGVLGSNLIGVHAVHLDEADIELLARHGCSVA
                     HCPTSNMKLASGIAPVPRLLAAGVPVGLGTDGAASNNRLDLLQEMRHAALLAKVGSLD
                     ATAVPAHAALRMATLGGARALGMDDRIGSIEKGKCADLCALDLSAPQCRPCFDPVSHL
                     VYVCGRENVSHVWIDGETRVDKGVSLLHINDTELLRLVSMWQTKLGN"
     gene            15623..16321
                     /locus_tag="PKV97_00975"
     CDS             15623..16321
                     /locus_tag="PKV97_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB04475.1"
                     /translation="MIKQSKKQMLMLAAIASIGLSAPSAFAQVSDVVVAGKGEIPYVI
                     DARNVVARSGTGLCWRTGYWSPAAASTALAGDLPVGCACDKDIVPADKCTVKAAAAAP
                     GPKPTADKVKLSADALFDFDKAVLKAEGKAKLDQLAAQAKSLKLEVILAVGHTDRLGS
                     NTYNQRLSERRAAAVKTYLVSKGVDANRVYTEGKGETQPVTGNKCDKVGGRAAQISCL
                     QPDRRVEVEVIGSK"
     gene            16429..17133
                     /gene="ubiG"
                     /locus_tag="PKV97_00980"
     CDS             16429..17133
                     /gene="ubiG"
                     /locus_tag="PKV97_00980"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HNB04476.1"
                     /translation="MNTVNADPAELQKFSDLAHRWWDPASEFRPLHEINPLRLGWIDG
                     KAALAGKKVLDIGCGGGILAEGMAALGAEVTGIDLSEKALSVARLHLFESGHKVDYRH
                     MSAEALAEESPGRFDVVTCMEMLEHVPDPASIVTACARLVKPGGHVFLSTLNRNPKSY
                     LFAIVGAEYLLKLLPAGTHEYARFIKPSELSRHCRNAHLDVQEIIGLSYNPLAKTYSL
                     GRDTDVNYLMHTVAAG"
     gene            17274..>19819
                     /locus_tag="PKV97_00985"
     CDS             17274..>19819
                     /locus_tag="PKV97_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNB04477.1"
                     /translation="MSFRHLKSLFNPRSIAVIGASARERRMGNVLMRNLLSGQFAGPI
                     MPVNPRRTSVAGVLTYPSIAALPQTPDLAIICTPAPLVPRLVEELGERGARAVMVMAN
                     QLDTTLGADGRPIERSILEITRRYEMRLLGGGTLGILVPGLGLNATFSQIAARPGKLG
                     FVSQRDTVGTMVLDWALRKKVGFSHFVSLGDSLDIGFGEVLDFLAADSGTRAILLYIE
                     SIQDRRAFMSAARAAARNKPVIAIKAGRSPDNPLMGVSDPLFLEMPNLVSHDDVHDAA
                     LRRAGILRVDQLEEMFGAAETVLRARPLRGNRLVVLSNGGGAGLMVEDSLYLAGYSLP
                     ALKEQTIERLRRFMSPAWNGRNPLEIRVDSEPACYERLLKILQEERDGDAVLLIHTPN
                     ALSSSIDTAQQVIATARETGINLMTCWVGDESVEKERNLFINAGIATFDSPEHAASAF
                     LHMHRHRIASEVLMEVPTSEPEAFKPDAARARAAIQGALAQSRASLTESESKDVLAAY
                     GVPVIQTHLAATPEEAAQIAGRIGLPVALTLMSRDIRRKWDVGGVALNLESTEAVQVA
                     ARGMIERAGRAEPPVSVSGFTVQRMVMRGHARQLLIGVSTDRLFGPVIVFGEGGRALE
                     IFRGLAVGLPPLNKPLADDLITRSKAAALLEARGHLPAADRDALALTLTKVSQIVVDH
                     PEIHEMDINPVFVDEHGVQALDAHMRLRPATAHEHRLAIQPYPKALEEPSRLRDGRPI
                     LLRPIRPEDEPAHYAFLSRLTREDFIYRFFNYIPEFPRREMARLTQIDYDREMAFIAT
                     ATGPDGKPETLGVSRAVADPDNHTAEFALVIRSDLKRQRLGSILMDKLVR"
     assembly_gap    19820..19919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20173..20844)
                     /locus_tag="PKV97_00990"
     CDS             complement(20173..20844)
                     /locus_tag="PKV97_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNB04478.1"
                     /translation="MNAKERILGKLRASLAGTTAVADDYDIGLVTDPWRYAADERIAR
                     LRRMLEAVHGETLVTTVAQWPQRVLEALAARGIDELLVSPATAHGRRLAEHVRAQDAA
                     LRLKGYENPVEAWKDELFFHTPASLTGTLGGIAATGSLVLWPSPQEPRLMSLVPPLHI
                     ALLNASEVRDNLFELMHAQRWTDGLPTNALLVSGPSKTADIQQTLAYGAHGPKELLVL
                     VIEDA"
     gene            complement(20868..22307)
                     /locus_tag="PKV97_00995"
     CDS             complement(20868..22307)
                     /locus_tag="PKV97_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HNB04479.1"
                     /translation="MSHVADTSAQPIAFTRNAREALADAQLRRNFRGAMDALMDKRRN
                     QFPDGDELERLRAFGNRVRARALSKLPDLLERLEANLVRNGVQVHWAETVDEANAIVH
                     GIAERHAASKVIKGKSMVSEEMEMNHYLGARGVDCLESDMGEFIVQLRAEKPSHIIMP
                     AIHLNAGQVAKLFHDKLAVEYTEDVDRLIQIGRETLRRNFFEADIGVSGVNFAIAETG
                     TLLLVENEGNGRMSTTVPPVHIAVTGIEKVVENLRDVIPLLSLLTRSATGQPITTYVN
                     MISGPRKADELDGPQEVHLVLLDNGRSQAFADGELRQTLNCIRCGACMNHCPVYTRIG
                     GHAYGTVYPGPIGKIVTPHMLGLETTRDLPTASSMCGACGEVCPVKIPIPSLLRRLRE
                     EAVRAPAAEPHHVRGQGAKHSRKETLVWRAWRTLNTSPGLYRAAMYAGTRLRGLMPAD
                     IGPWTRYRSAPKPAARSLHELAREHLGEQ"
     gene            complement(22304..23137)
                     /locus_tag="PKV97_01000"
     CDS             complement(22304..23137)
                     /locus_tag="PKV97_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNB04480.1"
                     /translation="MSPLSSAPANATRVAPPLEAARSYPAKPADVYLFGTCVIDVFFP
                     GAGMDAIRLLEREGIRVHFPQAQSCCGQPAYTSGYTDEAREVARAQLALFAGDWPVVI
                     PSGSCAGMFRHHYFELFKDEPETLQQVASLSARTFELAEFLLEVCKVRFEHTGAPLKL
                     ALHTSCSARREMNTHLHGRALLAQLAGVERVEHDHESECCGFGGSFSVRMPEISGAMV
                     QDKTAALKGSGAAEVVSADCGCLLNINGCFEKQGDTLRGEHLASFLLRRTTSASTGGV
                     R"
     gene            complement(23251..24951)
                     /locus_tag="PKV97_01005"
     CDS             complement(23251..24951)
                     /locus_tag="PKV97_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HNB04481.1"
                     /translation="MQPGTLAFLAFSPILLAAVLLVGLRWPAKRAMPVVYFLTAGIGL
                     YVWEMSLNRVLASTLQGLVITIGVLWIIFGAILLLNTLKYSGGITAIRAGFAKISPDR
                     RIQAIIIGWLFGCFIEGASGFGTPAAIAAPLLVAIGFPALAAVLLGMLVQSTPVSFGA
                     VGTPIIIGVNSGLDSATIGAQLAARGSSWDVFLQVITSNVAIIHAMVGTVIPLIMVMM
                     LTRFFGREKSWKAGFEVLPFAIFAGLAFTVPYAFTGVFLGPEFPSLLGGLVGLALVTT
                     AARFGFLVPKKTWDFADPKEWPSEWMGSVEMKLDQLPRHQPISIFRAWLPYVLVGALL
                     VLSRVFPEIGGAMKSVVIVFADILGEKGIKADFQPLYLPGGILVAVVLITVFLHGMHA
                     RSLVKAVGESSRVLLGAGFVLLFTVPMVRILINSGVNAADLPSMPIAMAKWVADSVGN
                     VYPLLAPSVGALGAFIAGSNTVSNMMLSQFQFGVATSLGISTAMVVAVQAIGAAAGNM
                     VAIHNVVAASATVGLLGREGAILRKTFWPTVYYVLMTGTVAMIAIYVLGVSDPLLAAS
                     "
     gene            25391..25612
                     /locus_tag="PKV97_01010"
     CDS             25391..25612
                     /locus_tag="PKV97_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04482.1"
                     /translation="MHEISPSDLKTILHSKRANLYYLEHCRVLVNGGRVEYVTDEGNR
                     SAYWNIPIANTKTIAAQAQSVRLHSAQNV"
     gene            25638..>26929
                     /locus_tag="PKV97_01015"
     CDS             25638..>26929
                     /locus_tag="PKV97_01015"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HNB04483.1"
                     /translation="MTASYFGYWGKARPHAAQGDECHLLPYHCLDVAAVGVTLLKRSP
                     ALRTLFTDALALDVDERLYSWFAFCLAIHDLGKFAESFQGQRSDLVYRLRGRVADPGK
                     PYGVRHDTLGWLLWDQHLKDRAVDQCWFGRHSEEMLEGTDSWLRATMGHHGLPPAAQG
                     YWRHHFAPDDVRAIEGFLGDLRVLFSTDAIWPTDHAIDPRTFDATSRLLSWWIAGVTV
                     LADWIGSNTDFFTYRSDQCPLDDYWLRAQERAVSALDTAGVLPVPCQERSFTALFPEI
                     AAPSPLQAWAIGTPIHSGPQLHLLEDVTGAGKTEAAVMLAHRMMTTGCADGFFIALPT
                     MATANAMYSRIADVYRMLFEGNPSLMLAHGQRNLVEAFAQSVLPAGQEEADVAQQDDT
                     ATARCNAWLADHNKRALLAPAGVGTIDQVLLATLYSKH"
BASE COUNT         4346 a         9479 c         8920 g         4084 t
ORIGIN      
        1 cagcagtagt cgaactcgat gccctggccg atgcggttgg gaccgccgcc gaggaccatg
       61 atcttcttgc gctcggtcgg catcgcctcg cactcgtcct cgtaggacga atacaggtag
      121 gcggtggagg tggcgaactc ggccgcgcag gtgtcgacgc gcttgaacac cgggcgcacg
      181 cccagcgtat gccgatgcag gcgcaccgcg gtctcgtcag cattgagcag cttggccagg
      241 cggcggtcgg agaagccctt cttcttgagc tcgcgcagct cggcggcctg cagcgccttc
      301 agcgagcgcc cggcgagcgc cttctcggag agcacgatgt cctcgatctg ggcgaggaac
      361 caggggtcga tcttggacag gttgaaaacc tgctcgaggc tcataccctc gcggaaagcc
      421 tggcccacgt accagatgcg ctgcgcgccc gggctggcga tctcgtgctc gagatcctcg
      481 cggtcggcct cgacctcgtc caggccatac acgccgacct ccagcccacg cagcgccttc
      541 tggaaggatt cctggaagct gcggcccatc gccatgacct cgcccaccga cttcatctgc
      601 gtggtcaggc ggtcgttggc ctgggggaac ttctcgaacg cgaagcgcgg gatcttggtg
      661 acgacgtagt cgatcgaagg ctcgaaggac gccggggtgg cgccaccggt gatgtcgttg
      721 cgcagctcgt cgagggtgta gcccacagcc agcttggccg cgaccttggc gatcgggaaa
      781 ccggtggcct tggaggccag cgccgacgaa cgcgacacgc gcgggttcat ctcgatgacg
      841 atcatgcgac cgtcctgggg attgatggcg aactgcacgt tcgagccgcc cgtgtccacc
      901 ccgatctcgc gcagcaccgc gatcgaggcg ttgcgcatga tctggtattc cttgtcggtg
      961 agcgtctgcg ccggcgcgac ggtgatcgag tcgccggtgt gcacgcccat cgggtcgagg
     1021 ttctcgatcg agcacacgat gatgcagttg tcggcgcgat cgcgcacgac ctccatctcg
     1081 tattccttcc agcccagcag cgactcctcg atcagcagct cgttggtcgg actcgcctcg
     1141 aggccacgct tgcagatctc ctggaactct tccatgttgt aggcgatgcc gccgccggtg
     1201 ccgccgagcg tgaagctcgg gcggatgatc accgggaagc cgatgccacc ctggacctgg
     1261 agcgcctctt ccatgctgtg ggcgatgccc gagcgcgcgg agccgagacc gatcctggtc
     1321 atcgcgtcct tgaacttgag gcggtcctcc gccttgtcga tcgcctcttc cgaagcgccg
     1381 atgagctcga cgccgtactt ggcgagcacg ccgtggcgac cgaggtcgag cgcgcagttg
     1441 agcgcggtct ggccgcccat ggtcggcagg atcgcgtcgg gacgctcctt gtcgatgatg
     1501 cgctcgacca cctgccaggt gatcggttcg atgtaggtga cgtcggccgt ctccgggtcg
     1561 gtcatgatgg tcgccgggtt cgagttgacc aggatgacct tgtagccttc ctcgcgcagg
     1621 gccttgcagg cctgggcgcc ggaatagtcg aactcgcagg cctggccgat gatgatcggg
     1681 ccggcgccga tgatcaggat ggtcttgatg tctgtacgtt tcggcattgt cttgcctcga
     1741 tgattcctcg acgccgcgac tgtcgcgcgc gtcaaaaact ggggggcagg ccgcagcctg
     1801 ccgcgggccg ggttacttgg cggcttccat cagaccgatg aagcggtcga acaggtaggc
     1861 gacgtcatgc ggcccggggc tcgcttccgg gtgtccctgg aaggagaagg cgggcacgtc
     1921 ggtgcgcgca atcccctgat tggtgccatc gaacaaggat acgtgcgtca ccttcaggtt
     1981 cgccggcatg gtcgccggat cgaccgcgaa gccgtggttc tggctggtga tcagcacttt
     2041 gccggtgtcg agatccttga ccggatggtt ggcgccatgg tgaccggtgg gcatcttcat
     2101 ggtcttcgcg cccgaggcca gcgccagcag ctggtggccg aggcagatgc cgaaggtcgg
     2161 catgcgcgcg gcgacgagct cgcggatcgc ggcgatcgcg tagtcgcagg gctcggggtc
     2221 gccggggccg ttggacagga acaccccatc cggattcagc gccagcactt cggatgccgg
     2281 cgtctgcgcc ggcaccaccg tgaggcggca gccgcgctcg accagcatgc gcaggatgtt
     2341 gtacttgacg ccgaagtcgt aggcgacgac gtggaagcgc ccgtcggcgc ggtcgctgta
     2401 gccgctcccg agcgcccact cggcctgctt ccattcatag cgctcggcga cactgacgac
     2461 cttggcgagg tccatgcccg cgaggccggg aaaggcgcgc gcctgggcca cggcagcctc
     2521 ggcgtcgagc gcctggcccg cggccgcagt gacgatgcag cccgcctgcg cacccttctc
     2581 gcgcagcacg cgggtgagct tgcgggtgtc gaggcctgcg atcgcgacca ccttctcggc
     2641 gcgcaggtag gcgtccaggc gctgggtcat gcgaaagttg gacggcagga tgggcaggtc
     2701 gcggatgacc agaccggccg cctgcacgcg cgaggactcg atgtcctcgg ggttgatgcc
     2761 ggtgttgccg atatgcggat aggtcagggt gacgatctgg cggcaatagc tcgggtcggt
     2821 caggatttcc tgataccccg acatcgaggt gttgaacacc acctcgccga ccgtgctgcc
     2881 aaccgcgccg atacccttgc cgtggaagac ggttccatcg gcgagcgcaa gaagggcggg
     2941 cgggaatgca ctcacgagaa gctcctggaa tctgcttggg aagtcatgcg ggcacgcgaa
     3001 cgacgcgccc gatatgtaca aaaagcggga cgggcctccg ggccgatccc gcttcgaaaa
     3061 cctttcaatt gtatatttcc ggcggattca aggcaaatcg cccgcgcacc tccgctcacc
     3121 tgccgcaggc cgtttcttcc cgcagaacct cgccgagcag ctcgatctcg cgcgccgcaa
     3181 ggcccgcctg gctggtcagc gcgcgcggat cgcccgccgc ggccagctcc tccagacggc
     3241 gcgcggcatc caccgcacgc tgcgccgaga acaccccgac cgagcccttg atcatgtgcc
     3301 cgagctcgcg caggcgcgcg aaatccatgt cggcggcgcc cttgtgcaag tcggcgaccg
     3361 tgcgcggcag atcgcgcagg aagaactcga cgagttgctc gaccaccccg tcctcgcccg
     3421 cgaacatcgc gcgcgcgtct tcgaggcagg cgatgcggcg acggtcggca ggatcgagct
     3481 cgagcagggt gatgtcgccg gcgacctcgt cggcatccgc cggacccagc acccgctcga
     3541 tcgcggcgaa cagcgcctcg gggcggaccg gcttggcgac gtagtcgtcc atgcctgcct
     3601 gcaggcagcg ctcgcggtcg ccctccatcg cgtgcgcggt catcgcgatg atcggcaccg
     3661 gcttccagtc gcccgcgagc acccagctgc gccgcgcctc gcgcgcgcgg atggcctgcg
     3721 tggcctcgat gccgcccatc acgggcatct gcacgtccat cagcaccacg tcgaagtgac
     3781 ggttgtcgat cgcctcgagg gcctcctcgc cgttgttcgc caccgtcacg ctgtggccga
     3841 cgtgctcgag catgcgcttg gccacggtct ggttgaccgg gttgtcctcg accaccaata
     3901 cctcgagcac gcgcgccggc ggctccatcc ccaccgtgga gcgcagctcg accggtgcga
     3961 gctcgaaggg cggcggctcg gcagcctcgc gctcgccgcc ctgcgccatg cgcagcacgg
     4021 ccagcaggtc ctcggccgag aagggcttgg cgaggcggta gccgagcccg agctcctcgc
     4081 agcgcagggc atcgttgcgc tgcgagtggc tcgccatcat cggaatgatg cggtccggcc
     4141 gcggccactc ggcggcgaag cgctgcgcca gcgcgaagcc gcccggctcc ggcaggtccg
     4201 catccatcag caggaacgcg aagggatcgc tgccgtccgc ggcgcgcgcc agcgcctgct
     4261 ccaccgccac gccactgcag gccgcctcgg cacgcagggc ggcctcctgc agggtcgcgc
     4321 acaggaaatc ggcgaaggcg gcgttcgcgc tcgccaccag cacccgcgtc ccggcgagtg
     4381 cgccgacgcg cagcggggtc gggtcggcga ccgcgcgcag cggcagggtc acctcgaagc
     4441 ggctgcccgc acccggcacg ctatcgacac cgatccggcc ccccatcagc tcagtgagct
     4501 ggcggcagat cgccagaccg aggccggtgc cgccgtactt gcgcgtggtc gaattgtccg
     4561 cctgcgagaa ggcaccgaag atcgcctcga gcttgtcggg cgcgatgccg cagccggtgt
     4621 cgcgcacgag gaactcgatg ctggccgcgc cgccgttgcg ctggacgcag cgcacgcgca
     4681 cctcgacctc gccgtgctcg gtgaacttga tcgcgttgcc gatgagattc accaggatct
     4741 ggcgcagccg ggtggggtcg ccgcgcagca ccgagggcac gtcgggcgcg acgtcgtagt
     4801 agagctccag ccccttctgc gaagcacgca gcgcgagcgc gcgacaggtc tccgacacca
     4861 ccgagtgcaa cgagaagtcg atctgctcga cgctcaggcg gccagcctcg atgcgcgaga
     4921 agtcgaggat gtcgttgatg atggtcagca aggcctcggc cgaggacttg accgtctgca
     4981 ggtactcgcg ctgctcgccc gcgatcgtgg agtcgagcag caggtcggtc atgccgatga
     5041 tgccgttcat cggcgtgcgg atctcgtggc tcatgttggc gaggaagtcg cccttggccc
     5101 ggctcgccgc ctcggccgcc tccttggcct ggagcagttc gatctcgcgc agcttgcgct
     5161 cggtgacatc acggtaggtg ccctcgacgc gcgcccagcg cccatcctcg ccgcggccgc
     5221 tcgcgttgcc gcacacctcg atccagtgcc agctcgcgtc ctgcgcgcga atgcggatct
     5281 ccgcgcgcag ctcggccgct tctccgccga ggtgggcgcg cagcgcctgc tgcagcgccg
     5341 ccctgccctc ctcgtccacc agcgccatcc agtcgcccgc atccccgccg tcgtaaccga
     5401 gcagcctgcg ccagcgttcg tcgagccgga tcgagccgcc acgcaggtcc atgtaccaca
     5461 cgccggcacc cgcattcgcc gacaccaggt cgaggtgccg gcgcgcgcgc gccaggctgc
     5521 gcagcgccgc atcgcgctcg gcgcgcaggc gggagagctc cgcctcgacg gaaccgggat
     5581 cgggaggact cccgcgcgat tcggccgcgc cgctcatggc aggcctcagc gcaggccgag
     5641 cacgtcctgc atgtcgtaga gccccttcgc acgaccggcg agaaagcgcg ccgcccgcat
     5701 cgagccgagc gcatagggca tgcggctgcc cgacttgtgg gtgatctcga tgcgctcgcc
     5761 gatgccggcg aacagcaccg tgtggtcacc caccacatcg ccgccacgga tcgtcgaaaa
     5821 gccgatggtc tcggcgctgc gctcgccggt gtggccctcg cggccataga cagcgcaggt
     5881 ctcgaggtcg cgtccaagct cgcgggcgac gacctcgccc atgcgcagag ccgtgccgga
     5941 gggggcatcg accttgaagc ggtggtgggc ctcgatgacc tcgacgtcgt agccctcgga
     6001 gaggatgcgc gcggcgacct cgagcaagcg gaacaccgcg ttcacgccca ccgccatgtt
     6061 gggcgcgaag accacgggaa cgtgctcggc agcggcggcg atcgcggtct tgccggcggc
     6121 atcgaaaccg gtcgtgccga tcaccatcgc cttgccggcg gcacgcgcga gcgcgaggtg
     6181 cgcgagcgtg ccctcgggtc gggtgaagtc gatcacgcaa tcggccgcgc cgatcgcggc
     6241 ggcggcatcg tcggtgatga gcacgccggt ggccacgccg gccatctcgc cggcatcgcg
     6301 accgatgaag ggggtgtcgc tgcgatcgaa ggcggcggcg agcacgacgc cctcctcctt
     6361 cagcgcggcc tcgatcagca ttctgcccat gcgcccggag gcaccggcga tcgcgactcg
     6421 aatctgggac atcggattgt tttcctgcat caatgggccg gcacgagcgg ccgccccgga
     6481 atggacccgg gcgccgcctc agcgcgcgct ggcggggggg atctcgacca cgcgcgagcg
     6541 ctcctgcgcc gccgcggcgg gcgcgcccga ggctgcggcg acatcgccct cgatgcggtc
     6601 gagcacgtcg cccgcgaaga agagcgagat cacgcgctgc tgcgcgtcgc cactgcccgg
     6661 ccggaagcga tagacgtagt cccagcggtc cttgcggaac atgtcgacca ccagggggga
     6721 gccgagcacg tagcgcacct gatcgcgggt catgccgcgg cgaagctgcg cgaccatctc
     6781 ctggtcgacg taattgcctt ggcggatgtc gatccggtag gggtcgacca ggccgacaac
     6841 ggagtcgaag gagcagcctg caagcaggcc ggaagagacg atgagcgcag cgaggaggga
     6901 acgcatggga gagtgggcgc cgggatgcga ccggggtcgc agttctcagt taggggcaaa
     6961 aaatataaca tagccgcgat cgacggcgct cgaccgccat tgccggagac tgcccgcggg
     7021 ggctcctgcc tgctgcggcg cacaccgtaa cgggctggca tcgacctccc gcgagtccct
     7081 ggacaacggt cggccaaacg gccttcgcag gctcccgcaa cccgatctcc cgcaagtcaa
     7141 cctcatccag agcccatgtc cgacaactcc aggaacctga aaagcatcgg cctgaaggcg
     7201 acctaccccc gcctgaagat cctcgacctc ttccagagct cggagcagcg ccacctgacc
     7261 gcggaggatg tataccgcac gttgatgaac gaaggcatgg acatcggctt ggccacggtc
     7321 taccgcgtac tgacgcagtt cgagcaggcc ggactgctcg agcgccacta cttcgagtcg
     7381 ggcaaggcgg tgttcgaact cgccgaaggc ggccatcacg accatctggt gtgcatgcag
     7441 tgcggcaagg tcgaggagtt cttcgacgcc gagatcgaac gtcggcagaa ccagatcgca
     7501 ctcgaacgcg gcttcgcgat ccgcgagcac gcgctctaca tctacgccga ctgccagcgc
     7561 gagaactgcc ccaacctcgg gcgcgagaag gactgaagcc gcgccggacc gaggcatcgc
     7621 gacggatgcg cgccagcgcc gagggcgcgt ggacgacgcg aaaatcagcg ccggccgagc
     7681 atctcggccg cgtgttctcg ggtggtcgcg gtgatattca cgccgccgag catgcgcgcg
     7741 acctcctcca cccgcgccgc gtcgtcgagc ggcgcgacct cgctcagcgt ctcgccgccc
     7801 tgctcgcgct tggcgatgcg ccactgccag tccgcgcagg ccgccacctg gggcaggtgg
     7861 gtgacgcaaa gcacctggcg atcctgcccg aggcgggcga gcagcttgcc gacgatctcg
     7921 gcgacgcgac cgccaatgcc cacgtccact tcgtcgaaga tcagcgtggg caccgcgctg
     7981 tcgcggctgg tgatgacctg gatggccagc ccgatgcgcg acagttcgcc gccggaagcc
     8041 accttggcga ggctgcgcag tggctggccg gcattcgcgg caacacggaa ctccacctcc
     8101 tcgacgcctg ccgcggaggg tgcgcaaggt gtgagcgcga cctcgaagcg gccacccgcc
     8161 atcgccaggt cctgcatcgc ggcggcgatc tcctccgaca gccggtgcgc ggcggggagg
     8221 cgcgccgcgg tgagctcggc cgcgacggac tcgaaggcgg cccgcgccgc ctgcgccgcc
     8281 gcggcaaggc gcgccggatc ggcggtcgct tccaatgtgt ccaggcgctc gcgccactgc
     8341 tgcgcgagcg cgggcagctc ctcgggctgg acgcgatact tgcgcgccgc ctccatgacc
     8401 gcggcgatgc ggcgctcgag ctcggcgaga cgtcccggat cgaggtcgag ccggtcacga
     8461 tagcggcgca gggcgtgcag ggcctcgtcg gcctggatgg cggcggcgga gatcagttcg
     8521 cgcacatccg ccagcccggc gtcgaagtcg gccagcgcag cgatgcggcc gtcgaggcgg
     8581 cccagcacgg agcacaccgc gagttccccc tcgccgagcg ccaccaccgc cgcctccgcc
     8641 ccctcgatca ggcccgcggc gtgggccagg cgagcgtgct cggcattgat ctccgcccat
     8701 tcctcgatgt cgaagccgac ctgctcgagc tcgcgcagct gccaggcgag cagctcgcgc
     8761 tcgcgcgccg agctcgccga gtcggcctcc gcttcgcgct gcaggcgcag caggcgctgc
     8821 cactcgcggt ggcgcgcggc cacctcggcg accagcgccc ccgcgcccgc ctgggcgtcg
     8881 agcagggcac gctgcgcgtc ggcgcgcagc aacgcgtggt gggcgtgctg accgtggatg
     8941 tcggccagcc actcgccggc ggtgcgaagc tgagcgagcg tggccgccac gccgttgatc
     9001 cacgcgcggc tacgaccacc ggcgtcgatg gtgcggcgca ggatcagcat gccctcgtcg
     9061 gcaggcagtt cctgctcgac gagccaggca gcgagtccct cgccggcggg caggtcgaat
     9121 tcggcggcca cgtcggcgcg ctcgcggccg ctgcgcacca tggctgcctc gccgcgcccg
     9181 ccgagcgcga gcccgagcgc atcgagcagg atcgacttgc cggcgccggt ctccccggtc
     9241 agcgcaccga agccggcggc gaattcgagg tcgaggcgat cgacgatgac gaagtcacgg
     9301 atggtcaggc ggcggagcat ggcagcggcg gtcgcgggtg gggttgggac gggacgacgg
     9361 ggcctgagga gtggcggcgc ggagatcggg cggcgggtgc gctcacaccc tcggcgccgc
     9421 gctccagtgc agcttctggc gcagcatcgc gaagtaggaa tagccctcgg gatgcagcag
     9481 gcgcaagctg cggctcgagc gtgtcatgcg cacgcagtcg cccgcgcgca ggtcgaaacg
     9541 ggtctggcca tcgaagtgca cccgggcgtc gtgcggcggc agcagcacga tgtcgatgcg
     9601 gcaggagtcg ggcagcgtga tcgggcgtgc ggtgagcgcg tgcgggcaca gcggcaccag
     9661 cgcgatcccg cccacgctcg ggtgcaggat cgggccgttg gccgacagcg cgtaggcggt
     9721 cgagccggtg ggggtggata cgatcatgcc gtccgagcgc tgggtgtaga cgaactcctc
     9781 gtcgatcgac agctcgaact cgatcatgcg cccgagctcg cccttgttca ccaccacgtc
     9841 gttgagggcg agcgtgtgga acacgcgtgc gccgccgcgc agcacctcga cgtcgagcat
     9901 gaaacggtcc tcggcggtga aggcgccgtc gaggatctcg gcgaggcgct cggtggccga
     9961 gctgcgcgcg atgtccgtga ggaagccgag gcggccgagg ttgacgccca ccagcgggac
    10021 gccgtgctcg atcagccggc gcgcggtgtg cagcatggtg ccgtcgccgc cgatcaccac
    10081 cgcgaggtcg gcgctcgcgc cgatatgctc gtagcttgcc accgggaagt cgtgggcgcc
    10141 gccgatggaa ctcgcggtgc cctgctcgat caacaccgca aggccgcgat cgcgcaggaa
    10201 gcgggcgatc gacagcaccg actcggccac gtcggggctc tgatatttgc cgatgagggc
    10261 aagcgtgcgg aatggcgtgc tcatgggcgc cgattaaacc acaatcgtcc ccgcgcttga
    10321 caccgccgcg cccgcgcgcg gtggcccgca gtggccagcg cgggcgtgct tcaatagaat
    10381 cggcctctcg actccaggca gccatgaatc cgctcgacgc acgctcccag atcctcctca
    10441 agaccctgat cgaacgccac atcgcggacg gccagccggt cggctcgcgc gcgctgtcgc
    10501 gctattccgg tctggagctg tcgccggcga cggtgcgcaa cgtgatgagc gacctcgagg
    10561 agatgggctt catcgccagc ccgcacacct cggcaggccg catcccgacc gcgctcggct
    10621 atcgtttctt tgtcgactcg ctgctcaccg tgcagccgct cgagaacgcc cgcgtgcgcg
    10681 ccctcgaagg ccagctgctg cccaaccagc cgcagcgcct gatcagctcg gcctcgcacc
    10741 tgctctccga actcacccag ttcgccggcg tggtggtggc accgcgcaag gacgcggtgc
    10801 gcatccgcca gttcgagttc atcagcctgg ccgaaaatcg catcctgctg atcatcgtca
    10861 ccaccggcgg cgacgtgcag aatcgcctgc tgatcacccg gcgcgcctac acgccgaccg
    10921 agctcaacgg cgctgccgcc tacctcaacg agcacttcgc cggcctgtcc ttcgaggaga
    10981 tccgccgccg cctcggcgac gagctgcgcc agctgcgcag cgacatgagc gaactgatga
    11041 ccgcgaccgt cgacgccggc tcagaagccg ccgaagacaa cgcggcgagc tacgtacttt
    11101 cgggcgaaac caagctgctc gacgtcgagg acctgtcgag caacatgtcg cagctgcgcg
    11161 agctcttcag actcttcgag cagcgcaccg gcctactcca gctgctcgac ctgtccaacc
    11221 gcgccgaggg cgtgcagatc ttcatcggtg gcgaatccgg cctcgccccg ctcgacggct
    11281 gcagcgtgat caccgcgccc tacgaggtcg acggccaggt ggtcggctcg ctcggtgtca
    11341 tcggccccac ccgcatggcc tacgaccgcg tgatcccgat cgtcgacatc accgcccggc
    11401 tgctctcgaa cgccctctcc tccaagcact gattccggcg tgcgcctgcc ttgccgtgcg
    11461 cacgccccca cccgaggacc acgatgcccc gttaccggcc cgaaccgatc caccgtcacg
    11521 acagcacgcc gcgcaccggc gtgctgctgg tcaacctggg cacccccgcc gcccccaccg
    11581 ccgccgcgct gcgtccctac ctcaagcagt tcctttccga cccgcgcgtg gtcgagatcc
    11641 cgcgcctggt gtggtggccg atcctcaacg gcatcatcct caacacgcgg cccaaggcct
    11701 cggcacagaa atacgccagc atctggaccg acgagggctc accgctgctg gtgcacaccg
    11761 tacgtcagac caagctgctc gccggctatc tcgccaagga cggctacgac gggctcgaca
    11821 tcgattacgc gatgcgctac ggcgcacccg cgatcggcga caccctggac gcgatgcgcg
    11881 cgcgcggctg cacccgcatc ctggtggtgc cgatgtaccc gcagtattcc gcgagcacca
    11941 ccgccaccgt ggtcgacgag gtcggcagca gcctggcgcg ctggcgcaac cagcccgaga
    12001 tgcgcttcgt gcgcaatttc cacgacgacc ccggctacat cgatgcgctg gtgaagagcg
    12061 tgcgcgagca ctgggcggcc aacggcgagc ccgagcgcct gctgctgagc ttccacggcg
    12121 tgccgcggcg ctcgctcgat ctcggcgacc cctatcactg cgagtgccac aagaccggcc
    12181 gcctcgtcgc cgaaggcctc ggcgtggcac gcgagcgcgt actggtcacg ttccagtcgc
    12241 gcttcggcaa ggcagaatgg ctcaagccct acacccagcc caccctggaa cagctcgccg
    12301 ccgagggcgt gcgccgcgtc gacgtgatgt gcccgggctt cgtcgcggac tgcctggaga
    12361 cgctggagga gatcgcgctc gagtgcaagg aagccttcct ggagaagggc ggccaggcct
    12421 tccactacat cccctgcctg aacgagcgcg acgcctggat caaggcgctg gccgcgctgg
    12481 tgcgcaagca cctgggcaac tggctggaga tcgccgcgcc ggacgccgcc gcgcgcagcg
    12541 ccgcactcga gcgcgcacgc acgctcggcg ccgggcagta gccgcggtac ccgcccgcac
    12601 aggctggccg aaacagcccg gctcggaggg attcaggcgg gtgcgggcgg actccacccg
    12661 caattcattc agtagtcgac gatctgcagg tcgtgcacga ccggcgccgc gcgcaccgca
    12721 cgcaccgccg cgggggcgtg ctcggccgcc tggcgcgaca ccgccgcaag ccggcgcacg
    12781 gcgacggtgc caagctggcg acggaagcgc tccagcactt cctcgctcgc aagcgcgagc
    12841 gcggcggggt tgacttcgag atagctgagc tcgtcgagcg acaccgcgct cagcagctgc
    12901 ttgtgctcga gctggtcgag ccaggcctgc tcgccgaaaa cctcgcccgg ccccacctcc
    12961 agcacccgcc gcccgccgat ggagagctcg accttgccct cgagcacgat cccgaagcgc
    13021 tgatcgaggt cgccctcgcg catcaggccg aggtcgctgc cgaccttgcg ccaggcgcag
    13081 atccgcatca cctcccacag atccacgtcc tcgaactcgg tgaagaaggc cagtttgcgc
    13141 aggcgcgcga agcgctcggc gtcgtggtgc acgtgacctt cgtcgaggat ctggtagcgg
    13201 accgcggcga ggtccttggc gaactccgcg ccgttgcggt agcgcgagta gaggtccttc
    13261 tcgagcgcct tgcggattac cgcgtccatc tgctcgggca cgtccgggtt gagctgcgag
    13321 accgagggcg gatcggcgtt gatgatctta tagacgagct gcgccggatt gctcgcgcgg
    13381 aagggcagcc gcccggtcag catgtggaac agcagcacgc cgagcgaata caggtcggtc
    13441 ttctggttga gcggatagcc cttgatctgc tccgggctca tgtaggccgg ggagcctacg
    13501 cccatgatga aggtcgagtc ggtgtcgagc ttcttgttga tgttgagcgc gaggccgaag
    13561 tcggtgatct tcacgttgtc ctgctcgtcg accaggatgt tcgccggctt gatgtcgcgg
    13621 tgcacgatgc cctggcggta agcgtggtcg agcgccatgc agcacttgaa cacgatcccg
    13681 atcgtgcgat ggatgggcag caggttctgg aagctgcagt agcgctccag cgagccgccc
    13741 gggaagtact ccatcaccac gtaggcctcg tcgggctcga tcgaggcatc gtggatacgg
    13801 atgatgttgg ggtggtcgag gcggcccgac accacgcgct cggccttcag cagcttgatc
    13861 aggcggcggt tccacttcgc ctcgtcgcgc gccttgtcgt cgaagcgcac gtgcttgagc
    13921 gccaccggct cggggtagtc cggggtctcg gcgagataga ccaccgcggt cgcgccgcgg
    13981 ccgatctcct tgatgatcct gtacttgccg atgcgttcgg gaatccggtt catgctgtct
    14041 tgcagggcgg gggcgcccgc gcggccggcg ggcttcggcg cgattgttgc acgatccggt
    14101 gccggcgtgc gcctccacac ccgcagcgac gcgcgggatt gtccgcccgg gccgcgcatg
    14161 cttgaatgag gctctccccg caactgcccg ccaggagccg ccttgagcga agccgtagac
    14221 ctgctgatca acgcccgctg gatcgtcccc gtcgaaccgc gcgacgctgt gctcgccgac
    14281 cacgcggtgg cgatccgcgg cggacgcatc gtcgacctcc tcggccagga cgaggcgcgc
    14341 acgcgctacg tcgcggacga ggtggtcgag ctgccgcggc acctgctgat ccccggcctg
    14401 gtgaacctgc acacccacgc cgcgatgagc ctgctgcgcg gcatcgccga cgacctgccg
    14461 ctgatgcgct ggctggagga ggcgatctgg ccggccgaga gccgtcacgt ttccgccgcc
    14521 ttcgtgcgcg acggcaccct gctcgccgcg gccgagatga tccgtggcgg catcaccacc
    14581 tgcagcgaca tgtacttcca ccccgaggcg gcggcggaag ccttcgccgc cgcgggcatg
    14641 cgcgcggtgg tcggcgcggt cgtgctggaa ttccccacct cgtacgcgag cgaccccgag
    14701 gactacctgc gcaagggcct ggccgcccgc gaccgctggc agggccaccc gcggctcggc
    14761 ttctcgatcg ccccgcacgc gccctacacc gtctcggacg acagcttcca ccaggtgcag
    14821 accctcgcgg acgaactcgg cctgccgatc catgtgcaca tccacgagac cgcacaagag
    14881 atcgccgact ccctcgccgt ccacggctgc cggccgctcg ctcgcctcgc gcgcctcggc
    14941 gtgctcggca gcaatctgat cggcgtccat gcggtgcatc tggacgaggc cgacatcgaa
    15001 ctgctcgccc gccacggctg cagcgtcgcg cactgcccca cctcgaacat gaagctcgcc
    15061 agcggcatcg cgccggtgcc gcgcctgctc gccgccggcg tcccggtcgg cctcggcacg
    15121 gacggcgcgg cgagcaacaa ccgcctcgac ctgctccagg agatgcgcca cgccgcgctg
    15181 ctcgccaagg tcggcagcct ggatgccacc gcggtaccgg cgcatgccgc cttgcgcatg
    15241 gccacgctcg ggggcgcgcg cgcattgggc atggacgatc gcatcggctc gatcgaaaaa
    15301 ggcaaatgcg ccgatctttg cgcactcgac ctttccgcac cgcaatgccg gccctgtttc
    15361 gatccggtgt cgcatctcgt ctacgtatgc ggtcgcgaaa acgtctccca cgtgtggatc
    15421 gacggcgaaa cccgcgtgga caaaggcgtc tcgctgttgc atattaacga caccgaattg
    15481 ctccggctcg tgtcgatgtg gcaaactaag ctcggtaatt gaactggata tactggtaca
    15541 aaccgacacg aagacggcag attcgccagt cttcatttcg ttcagtggca attccggcga
    15601 ctcgcccttc aaagaggaaa ccatgatcaa acaatccaag aagcagatgc tcatgctggc
    15661 cgccatcgcc tcgatcggcc tgtccgcccc gagcgctttc gctcaggtca gcgacgtcgt
    15721 cgtcgccggc aagggtgaaa tcccctacgt gatcgacgcg cgcaacgtcg tcgctcgcag
    15781 cggcaccggc ctgtgctggc gcaccggcta ctggagcccc gccgccgcct cgaccgccct
    15841 cgccggcgac ctgccggtcg gctgcgcttg cgacaaggac atcgtccccg ccgacaagtg
    15901 caccgtgaag gctgctgccg cggctccggg tccgaagccc accgccgaca aggtcaagct
    15961 gtcggccgac gccctgttcg acttcgacaa ggccgtcctg aaggctgaag gcaaggccaa
    16021 gctcgaccaa ctcgccgccc aggccaagtc gctcaagctg gaagtgatcc tcgccgtcgg
    16081 ccacaccgac cgcctgggtt ccaacaccta caaccagcgc ctgtccgagc gtcgcgccgc
    16141 cgccgtcaag acctacctgg tctcgaaggg cgtcgatgcc aaccgcgtct acaccgaagg
    16201 caagggcgaa acccagcccg tgaccggcaa caagtgcgac aaggtcggtg gccgcgccgc
    16261 gcagatctcc tgcctgcagc cggatcgtcg cgtcgaagtg gaagtcatcg gctccaagta
    16321 attccggagt cggtaacccg aaagccctgc ctcggcgggg ctttttcgtt tgtgccgggc
    16381 gcgcgcgcac ccggtgcccg tgcgtgcaag caaggaccct cgaacgccat gaacaccgtc
    16441 aacgccgatc ccgccgaact gcagaaattc agcgacctcg cccaccgctg gtgggacccc
    16501 gcctccgagt tccgtccgct gcacgagatc aacccgctgc gcctgggctg gatcgacggc
    16561 aaggccgcgc tcgccggcaa gaaggtgctc gacatcggct gcggcggcgg catcctcgcc
    16621 gagggcatgg ccgcgctcgg cgccgaggtg accggcatcg acctatccga gaaggcgctc
    16681 agcgtcgccc gcctgcacct tttcgagagc ggccacaagg tcgactaccg ccacatgagc
    16741 gcagaggcgc tcgccgagga atcgcccggc cgcttcgacg tcgtcacctg catggaaatg
    16801 ctcgagcacg tccccgatcc ggccagcatc gtcaccgctt gcgcccggct ggtaaaaccc
    16861 ggcggccacg tcttcctgtc cacgctcaac cgcaatccga agtcctacct gttcgcgatc
    16921 gtcggcgccg aatacctgct caagttgctc ccggccggca cccacgagta cgcccgcttc
    16981 atcaagccct ccgaactcag ccgccattgc cgcaatgcgc acctggacgt gcaggagatc
    17041 atcggcctgt cctacaaccc gctcgccaag acctactcgc tcggccgcga cacggacgta
    17101 aactatctga tgcacaccgt cgccgccggc tgaacctcgt ccggacagct cgagttcgac
    17161 gcggagaacc gggcgctggc gggccgctcg acccggccgc aattcagttc atgcatgtgc
    17221 ggcaggcatg cattaaactg cagacgagcg attcctcccc ggggagcaag gcgatgagct
    17281 tccgccacct gaaaagcctg ttcaatccac gctcgatcgc cgtgatcggc gcgagcgcgc
    17341 gcgagcgccg catgggcaac gtgctcatgc gcaacctgct gtcgggccag ttcgccggcc
    17401 cgatcatgcc ggtcaatccc cgccgcacct cggtggcggg cgtgctcacc tacccgagca
    17461 tcgccgcgct gccgcagacg cccgacctgg cgatcatctg cacccccgcg ccgctcgtgc
    17521 cccgcctggt ggaggagctc ggcgaacgcg gcgcgcgtgc cgtgatggtg atggccaacc
    17581 agctcgacac cacgctcggc gccgacggcc gtccgatcga gcgcagcatc ctcgagatca
    17641 cccgccgcta cgagatgcgc ctgctcggtg gcggcacgct gggcatcctg gtacccggac
    17701 tcgggctcaa cgcgaccttc tcgcagatcg ccgcgcgccc cggcaagctc ggcttcgtct
    17761 cccagcgcga cacggtcggc accatggtgc tcgactgggc gctgcgcaag aaggtcggct
    17821 tctcgcactt cgtctcgctc ggcgacagcc tggacatcgg cttcggcgag gtgctcgact
    17881 tcctcgccgc cgactcgggc acgcgcgcga tcctgctcta catcgaatcc atccaggatc
    17941 gccgcgcctt catgtcggcc gcgcgcgccg ccgcgcgcaa caaaccggtg atcgcgatca
    18001 aggccggacg ctcgcccgac aatccgctga tgggcgtctc cgacccgctc ttcctcgaga
    18061 tgcccaacct ggtcagccac gacgacgtgc acgacgcggc gctgcgccgc gccggcatcc
    18121 tgcgggtgga tcagctcgaa gaaatgttcg gcgccgccga gaccgtgctg cgcgcccgcc
    18181 cgctgcgcgg caatcgcctg gtcgtgctga gcaacggcgg cggtgccggc ctgatggtgg
    18241 aggacagcct ctaccttgcc ggctacagcc tgccggcgct caaggagcag accatcgagc
    18301 gcctgcgccg cttcatgtcg ccggcctgga acggacgcaa tccgctcgag atccgcgtcg
    18361 actccgagcc ggcctgctac gagcgcctgc tcaagatcct gcaggaagag cgcgacggcg
    18421 acgccgtgct gctgatccac accccgaacg cgctctccag cagcatcgac accgcgcagc
    18481 aggtcatcgc caccgcgcgc gagaccggca tcaacctgat gacctgctgg gtgggcgacg
    18541 agagcgtgga gaaagagcgc aacctcttca tcaacgccgg catcgccacc ttcgactcgc
    18601 ccgagcacgc ggccagcgcc ttcctgcaca tgcaccgcca ccgcatcgcc tccgaggtgc
    18661 tgatggaggt gccgacctcc gaacccgaag ccttcaagcc cgacgccgcg cgcgcgcggg
    18721 cggcgatcca gggcgcgctc gcccaatcgc gtgccagcct gaccgagtcc gaatccaagg
    18781 acgtcctcgc ggcctacggc gtgcccgtca tccagaccca cctcgccgcc acccccgaag
    18841 aggccgcgca gatcgccggc cgcatcggcc tgccggtggc cttgaccctg atgtcgcgcg
    18901 acatccggcg caagtgggac gtcggtggcg tcgcgctcaa cctcgagagc accgaggcgg
    18961 tccaggtcgc tgcacgcggc atgatcgagc gcgccggccg cgccgagccg ccggtgtcgg
    19021 tgagcggctt caccgtgcag cgcatggtga tgcgcgggca cgcgcgccag ctgctgatcg
    19081 gcgtcagcac cgaccggctg ttcggaccgg tgatcgtgtt cggcgaaggc ggtcgcgcac
    19141 tggagatctt ccgtggccta gcggtcggcc tgccgccgct caacaagccg ctcgccgacg
    19201 acctcatcac ccgcagcaag gcggcagcgc tgctcgaggc gcgcggccac ctccccgcgg
    19261 ccgaccgcga cgcgctcgcg ctcaccctca ccaaggtctc gcagatcgtc gtcgaccatc
    19321 ccgagatcca cgagatggac atcaacccgg tgttcgtcga cgagcacggc gtgcaggcgc
    19381 tcgacgccca catgcgcctg cgtccggcca ccgcccacga gcaccggctg gcgatccagc
    19441 cctacccgaa ggcgctcgag gagccctctc ggctgcgcga cggccgcccg atcctgctgc
    19501 gcccgatccg ccccgaggac gagccggcgc actacgcctt cctgtcgcgg ctcacccgcg
    19561 aggacttcat ctatcgcttc ttcaactaca tccccgagtt cccgcgtcgc gagatggcgc
    19621 gcctgacgca gatcgactac gaccgcgaga tggccttcat cgccaccgcc accggccccg
    19681 acggcaagcc cgagaccctg ggcgtttcgc gcgcggtggc cgacccggac aaccacaccg
    19741 ccgagttcgc cctcgtcatc cgttccgacc tcaagcgcca gcgcctgggc tcgatcctca
    19801 tggacaagct ggtgcgctan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    19921 cgggtgcggg aagcgcggcg atgggccgat ctcggacatc gcgacttgcg tcgctcctac
    19981 ggaagcggct gcgggtttgg ggtgcgtggc gagggaccgg tcgccacgat cgcgcctgga
    20041 cggacccgcg gaaacacctc cgcatcgccg gagccgcgcg gtaggagcga cgcgagtcgc
    20101 gattccgccg ctccggcaag ccgcggcgcg gatcgtcggc acggccgggc gcgcccacga
    20161 tgcgcgccgc gctcaggcgt cctcgatcac cagcaccagc agctccttcg gcccgtgcgc
    20221 accgtacgcc agcgtctgct ggatgtcggc cgtcttggac ggccccgaca ccagcagcgc
    20281 gttggtcggc aggccgtcgg tccagcgctg cgcgtgcatg agctcgaaga ggttgtcgcg
    20341 cacctcgctc gcgttcagca gcgcgatgtg cagcggcggc accaggctca tcaggcgcgg
    20401 ctcctgcggg ctcggccaca gcaccaggct gccggtggcg gcgatgccgc cgagcgtgcc
    20461 ggtaaggctg gcgggcgtat ggaagaagag ctcgtccttc caggcctcca ccgggttctc
    20521 gtagcccttc aggcgcagcg ccgcatcctg tgcacgcacg tgctccgcca ggcggcggcc
    20581 gtgcgcggtg gcaggcgaaa ccagcagctc gtcgatgccg cgcgcggcga gcgcctccag
    20641 cacgcgctgc ggccactgcg ccaccgtggt gacgagcgtc tcgccatgca ccgcctcaag
    20701 catgcggcgc aggcgcgcga tgcgctcgtc cgccgcatag cgccagggat cggtgaccag
    20761 gccgatgtcg tagtcgtcgg cgaccgcggt cgtgcccgcg aggctggcgc gcagcttgcc
    20821 gaggatccgt tctttcgcat tcatgggggt ttcgctcctg gcgtcgttca ttgctcgccc
    20881 agatgctcgc gggcgagctc gtgcaggctg cgcgcggccg gtttcggcgc gctgcggtag
    20941 cgcgtccacg gcccgatgtc ggccggcatc agcccgcgca ggcgggtgcc cgcgtacatc
    21001 gccgcgcggt acaggcccgg agaggtgttg agcgtgcgcc acgccctcca caccagggtc
    21061 tccttcctgg agtgcttggc gccctgcccg cgcacgtgat gcggctcggc cgcgggcgca
    21121 cgcaccgcct cctcgcgcag gcggcgcagc agggacggga tggggatctt caccgggcac
    21181 acctcgccgc aggcgccgca catcgacgag gccgtgggca ggtcgcgggt ggtctccagc
    21241 ccgagcatgt gcggggtgac gatcttgccg atcggcccgg gatacaccgt gccgtacgcg
    21301 tggccgccga tccgcgtata caccgggcag tggttcatgc acgcgccgca gcggatgcag
    21361 ttgagcgtct ggcgcagctc gccgtcggcg aaggcctggc tgcggccgtt gtcgagcagc
    21421 accaggtgga cttcctgcgg gccgtcgagc tcgtcggcct tgcgcgggcc ggagatcatg
    21481 ttgacgtaag tggtgatcgg ctggccggtg gccgagcgcg tcagcagcga cagcagcggg
    21541 atcacgtcgc gcaggttctc gaccaccttc tcgatgccgg tgacggcgat gtgcaccggc
    21601 ggcaccgtgg tcgacatgcg gccgttgccc tcgttttcca ccagcagcag cgtgccggtc
    21661 tcggcgatcg cgaagttaac ccccgagacg ccgatgtcgg cctcgaagaa gttgcgccgc
    21721 aaggtctcgc ggccgatctg gatgaggcga tcgacgtcct cggtgtactc gaccgcgagc
    21781 ttgtcgtgga acagcttcgc gacctggcct gcgttgaggt ggatcgccgg catgatgatg
    21841 tgcgagggct tctccgcgcg cagctgcacg atgaactcgc ccatgtcgga ttcgaggcag
    21901 tcgacgccgc gcgcaccgag gtaatggttc atctccattt cctcggacac catcgacttg
    21961 cccttgatca ccttactcgc cgcgtggcgc tcggcgatgc catgcacgat ggcgttggcc
    22021 tcgtccaccg tctccgccca gtgcacctgc acaccattgc ggacgagatt ggcctcgagg
    22081 cgctcgagca gatcgggcag cttggacagc gcgcgcgcgc gcacgcggtt gccgaaggcg
    22141 cgcaggcgct cgagctcgtc gccgtcggga aactggttgc ggcgcttgtc catcagcgca
    22201 tccatggcgc cgcgaaagtt gcgccgcagc tgcgcgtcgg cgagcgcctc gcgcgcgttg
    22261 cgggtgaagg cgatcggctg cgcggaggtg tcggcgacgt ggctcatcgc acccctcccg
    22321 tgctcgcgga agtcgtgcgg cgcagcagga agctcgccag gtgctcgccc cgaagcgtgt
    22381 cgccctgctt ctcgaagcag ccgttgatgt tgagcaggca gccacagtcc gcgctcacca
    22441 cctcggccgc gcccgaaccc ttcagcgcgg cggtcttgtc ctgcaccatg gcgcccgaga
    22501 tctcgggcat gcgcacgctg aagctgccgc cgaagccgca gcactcgctc tcgtggtcgt
    22561 gctcgacgcg ctcgaccccc gccagctgcg cgagcagcgc gcggccatgc aggtgggtgt
    22621 tcatctcgcg ccgcgccgaa caggaggtgt gcagcgcgag cttgagcggc gcgccggtgt
    22681 gctcgaagcg gaccttgcac acctccagca ggaactcggc gagctcgaag gtgcgcgcgg
    22741 acagcgacgc gacctgctgc agcgtctcgg gctcgtcctt gaagagctcg aagtagtgat
    22801 ggcggaacat gccggcgcac gagcccgacg ggatcaccac cggccagtcg ccggcgaaga
    22861 gcgcgagctg cgcgcgcgcc acctcgcgcg cctcgtcggt gtaccccgag gtgtaggcgg
    22921 gctgcccgca gcagctctgc gcctgcggaa aatgcacccg gatgccctcg cgttcgagca
    22981 ggcggatcgc gtccatcccg gcaccgggaa agaacacgtc gatcacgcag gtgccgaaca
    23041 ggtacacgtc ggccggcttg gccggatagc tgcgcgcggc ctcgagcggc ggcgcgacac
    23101 gcgtggcgtt ggcgggtgcg gaggacagag gactcatgcg cttcggctcc ggaatttcat
    23161 cgttctgaag tgcgacgcag ccgcggccgg gccgcgacgg caggaaggac ggatgcgcgg
    23221 gggacgccga tgcgtccctc cgcgcgggac tcagctcgcg gcgagcagcg gatcggacac
    23281 cccgaggacg tagatcgcga tcatcgccac cgtgccggtc atcagcacgt aatacacggt
    23341 cggccagaag gtcttgcgca ggatcgcgcc ctcgcggccg agcagaccca ccgtggccga
    23401 ggccgccacg acgttgtgga tcgccaccat gttgccagcg gccgcgccga tcgcctgcac
    23461 cgccacgacc atcgccgtgg agatgccgag cgaggtcgcc acgccgaact ggaactggct
    23521 cagcatcatg ttgctgaccg tgttcgagcc ggcgatgaag gcaccgagcg cgcccaccga
    23581 tggtgccagc agcggataca cgttgcccac gctgtcggcc acccacttgg ccatcgcgat
    23641 cggcatgctg ggcaggtcgg cggcgttcac ccccgagttg atcaggatgc gcaccatcgg
    23701 cacggtgaac agcagcacga agccggcgcc gagcagcacg cgcgaagact cgcccaccgc
    23761 cttcaccagc gagcgcgcgt gcatgccatg caggaacacg gtgatcagca ccaccgccac
    23821 caggatgccg ccgggcaggt agagcggctg gaagtccgcc ttgataccct tctcgccgag
    23881 gatgtcggcg aacacgatca ccaccgactt catcgccccg ccgatctcgg ggaacacgcg
    23941 gctcagcacg agcagcgcgc ccaccagcac gtagggcagc caggcgcgga agatgctgat
    24001 cggctggtga cgcgggagct gatcgagctt catctcgacg ctgcccatcc actcgctcgg
    24061 ccactccttc gggtcggcga agtcccaggt cttcttcggc accaggaaac cgaaacgcgc
    24121 cgcggtggtg acgagggcca ggccgaccag gccgccgagc agcgagggga actcaggccc
    24181 gaggaagacg ccggtgaagg cgtacgggac ggtgaaggcc agacccgcga agatcgcgaa
    24241 gggcagcacc tcgaagccgg ccttccagct cttctcgcgg ccgaagaagc gcgtcagcat
    24301 catgaccatg atcagcggaa tgacggtgcc gaccatggca tggatgatcg ccacgttgct
    24361 ggtgatcacc tgcaggaaga cgtcccagct cgagcccctg gcggcgagct gcgcgccgat
    24421 cgtcgcactg tcgaggccgc tattgacgcc gatgatgatc ggcgtgccca cggcaccgaa
    24481 ggacaccggc gtgctctgca ccagcatgcc gagcagcacc gccgccagcg ccgggaagcc
    24541 gatcgccacc agcagcggcg cggcgatcgc cgccggcgtg ccgaagcccg aggcgccctc
    24601 gatgaagcag ccgaacagcc agccgatgat gatcgcctgg atgcgacggt cggggctgat
    24661 cttggcgaag ccggcacgga tcgcggtgat gccgcccgag tacttcaggg tgttgagcag
    24721 caggatggcg ccgaagatga tccacagcac cccgatcgtg atcaccagcc cctgcagggt
    24781 cgaggcgagc acgcggttca ggctcatctc ccacacatac aggccgatgc ccgcagtgag
    24841 gaaatagacg accggcatcg cgcgcttggc cggccagcgc aggcccacca gcagaaccgc
    24901 ggcgagcagg atcggagaaa aggcgaggaa tgcgagcgta ccaggctgca tgggagtccc
    24961 cgcctcagac cggcgcgcgc gtcacgcgcg cagccatgaa catcgatcga accatcattg
    25021 cctcttcccc cttcggatgg atttcatgaa atctgaatga ctgcgcttcc gtcttttcgg
    25081 taaattggta ttaccacttt accgaaagtt gcaccagact aagggagccc gcgaggcatg
    25141 tcaatggaaa tcatcagatt tatcgatggg aacatgagag gcggattcgg cgggcgcccg
    25201 gaggcagcgg cgggcggggg tgccgcaccg cacaggcgct ggtccgatgc tgcgcggcgt
    25261 cgaatgcggg gaatcaccgg acccggtgcc gccccccgag cagctcgcac gtgtcggaag
    25321 caggtcggca gcgccatgca tctatacttg tccggcaatg ccagttgctt cacctggaga
    25381 ccgatctgcg atgcacgaaa tctcgccatc cgacctcaag accatcctcc actccaagcg
    25441 cgccaacctg tactacctgg agcattgccg cgtgctggtg aatggcggcc gggtggaata
    25501 cgtcaccgac gagggcaacc ggtcggcgta ctggaacatc ccgatcgcca acaccaaaac
    25561 gatcgctgcg caggctcagt ctgtgagact gcactcagcc caaaatgtgt gagaactgca
    25621 acctcggaag aatccagatg acagcttctt acttcggcta ctggggcaaa gcacggccac
    25681 acgcggcgca aggtgacgaa tgccacctgc tgccctatca ctgcctcgat gtggctgcgg
    25741 taggagtcac cctgctgaag cgctcgcctg cactgcgcac acttttcaca gacgcactag
    25801 ccttggatgt ggatgagcgc ttgtattcgt ggttcgcttt ctgcctcgcg atacacgacc
    25861 tgggcaagtt cgccgagtcc ttccagggac agcgttccga tctcgtttac cgcctacgcg
    25921 gacgggtggc cgatccaggc aaaccatacg gtgttcgtca tgacaccctt ggctggctgc
    25981 tctgggacca acacctcaag gaccgggcgg tcgatcaatg ctggttcggc cgacacagcg
    26041 aggaaatgct cgaaggtacg gattcgtggt tgcgcgccac catgggacac cacggactgc
    26101 cccctgccgc tcaggggtac tggcgccacc acttcgcccc tgacgacgtt cgcgcgatcg
    26161 agggcttcct gggggacctc cgcgtccttt tctcaacgga tgcgatctgg ccgaccgacc
    26221 acgcaatcga cccgcggacc ttcgacgcaa ccagcagatt gctgtcgtgg tggatcgccg
    26281 gagtcacggt actcgcggat tggatcgggt cgaatacgga cttcttcacc tatcgttcgg
    26341 atcagtgccc actggacgac tactggctga gagcgcagga gcgcgccgtg tcagcactcg
    26401 ataccgcagg tgtattgccg gtgccatgcc aagaacgcag tttcaccgcg ttgttcccgg
    26461 aaatcgcagc gccttctccc ctgcaggctt gggcaatcgg cacgcccatc cattcagggc
    26521 cacaactcca tctgctggag gacgttacgg gcgccggaaa gaccgaggcg gccgtcatgc
    26581 tcgcacaccg gatgatgacc actggctgtg ccgacggctt cttcatcgcg ctaccgacca
    26641 tggccacggc caatgcaatg tacagccgca tcgccgatgt ctatcgcatg ttgttcgagg
    26701 gcaaccccag cctgatgctg gcacacgggc agcgcaacct ggtcgaggcc tttgcccagt
    26761 cggttctgcc agcgggccag gaggaggccg acgtggcgca gcaagacgat acagccaccg
    26821 ctcgctgcaa tgcctggctt gccgaccaca acaagcgtgc cctgctggcc ccggccgggg
    26881 tcggcacgat cgatcaggta ttgcttgcca cgctctacag caagcacca
//
LOCUS       DAOQNN010000006        25786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_585_length_25786_cov_9.485873, whole genome shotgun sequence.
ACCESSION   DAOQNN010000006 DAOQNN010000000
VERSION     DAOQNN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 25786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25786
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_585_length_25786_cov_9.485873"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PKV97_01020"
     CDS             complement(<1..1028)
                     /locus_tag="PKV97_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB04484.1"
                     /translation="MNEDKPSNRTARPRWGRRALLILVPLLLAGAWYGRSAFIGERDP
                     TTNYQFTTVQRGDVEDVVTATGTLQPRDYVDVGAQVSGQLRKIHVEVGDTVEAGQLLA
                     EIDPTVYRARVDASRAQLKNLRAQLKDREAQLALAQIQQRRQRALMAEDATTKESLQT
                     AEASAMSAEAQLEALRAQIEQIESTLRADEANLQYARILSPMAGTVVSITARQGQTLN
                     TNQQAPVVMRIADLSTMTVQTQVSEADIGRLKLGMEAYFTTLGGSGKRWQGKLEKIEP
                     TPTVTNNVVLYNALFDVLNPDGRLMTQMTAQVFFVVAQAKDALLIPMAALAQGQARPP
                     QAGAGPAPA"
     gene            1313..2314
                     /locus_tag="PKV97_01025"
     CDS             1313..2314
                     /locus_tag="PKV97_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNB04485.1"
                     /translation="MMKLKATSVAVALVVAGSAWAAVAVRDEPIKPIEPAKVENAAKV
                     ELGKKLWFEPRLSMSGIISCNTCHNLSMGGTDNLKTSIGHGWKAGPVNSPTVYNSSLA
                     IAQFWDGRAADLKEQAGGPIQAGVEMNMPHTLAVGILQSIPGYVEEFESVYGKNKIDI
                     DMITDSIAEFEKTLVTPDSRFDKWLRGDDKALSEKELAGYTLFKESGCVACHNGPAAG
                     GTSFQRMGVVEPYKSSSPAEGRSAVTGADADRFNFKVPTLRNVALTYPYFHDGEAATL
                     EQAVDTMGRLQLGRTYSKDEIGQIVAFLKTLTGELPQIAMPVLPPSSNATPRPDPFK"
     gene            2487..2906
                     /locus_tag="PKV97_01030"
     CDS             2487..2906
                     /locus_tag="PKV97_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNB04486.1"
                     /translation="MMNTYASSDAIACLRQAGIPVTHQRIEIARALFSAPVHLSADQI
                     WAKVLEASPDVSRATVYNTLRLFSEKKMIRELFVDPQRVVYDSTTAPHHHLYNIDSGE
                     VRDISDDELRVIGQPRLPAGVVLDAIDVIVRVRNRAA"
     gene            complement(3012..3428)
                     /locus_tag="PKV97_01035"
     CDS             complement(3012..3428)
                     /locus_tag="PKV97_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB04487.1"
                     /translation="MAFGGFSQGETRAPQSDINMVPLIDVMLVLLIVFMITAPLLTHS
                     VKIDLPQASSAPTEQKPETVALSIDGDGRVFWNNEAVDEVALPARLSAAAAQKPQPEL
                     HLRADRNTRYEVIAEVMAEARSAGIEKMGFVTVPER"
     gene            complement(3428..4189)
                     /locus_tag="PKV97_01040"
     CDS             complement(3428..4189)
                     /locus_tag="PKV97_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB04488.1"
                     /translation="MDSSQAFGLAHLWAQSDNIIRAVALILLVMSITSWYLILSRSLR
                     QLRARSHSGAVEAFWAAPDLDAGLRVLAERAPESPFEALAQQGAAAAQHVRRHTHSNT
                     LGGKLDTDEVITRALRKSIALSTAALESGLTVLASIGSTAPFVGLFGTVWGIYHALVN
                     ISVSGMATLDKVAGPVGEALIMTAFGLFVAIPAVLAYNGITRANRVELSELDAFAHDL
                     HAWFATGARIAAVPGAAPRAAEVATGTPAGAHGAA"
     gene            complement(4192..4953)
                     /locus_tag="PKV97_01045"
     CDS             complement(4192..4953)
                     /locus_tag="PKV97_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNB04489.1"
                     /translation="MRAPASGAEVAPRPTAAARGSSPLRRGALLGLIVAAHLGGLAML
                     ARLAGERPVPAELMPIQVALIAAPAVAEPLPAPPEPVVEPPRPPEPRPVARPPEPTPP
                     TPPMVKKPTPKPVVKKPAPVTESPTALSAAEEPAAPPPPAPAPPAAAPAATAAPATVT
                     AARFDAAYLNNPHPAYPALSRRLREEGQVTLRVLVSPDGQPAQVELRTSSGSERLDRA
                     AREAVARWRFVPARRGDSAIESWVLVPIVFKLQGN"
     gene            complement(5104..5583)
                     /gene="bfr"
                     /locus_tag="PKV97_01050"
     CDS             complement(5104..5583)
                     /gene="bfr"
                     /locus_tag="PKV97_01050"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNB04490.1"
                     /translation="MKGDKKVIQYLNKQLTIELTAINQYFLHARMYKNWGFTRLGKHE
                     YDESIEEMKHADMLIERVLFLEGLPNLQNLDKLLIGENVPECMQGDLKLELGGRANLI
                     EAIAYCESSKDYVSRELFEHILEDTEEHIDYLETQLGLVDSVGLQNYLQSQMDPGAS"
     gene            complement(5580..5819)
                     /locus_tag="PKV97_01055"
     CDS             complement(5580..5819)
                     /locus_tag="PKV97_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNB04491.1"
                     /translation="MYVCVCNAVTERQIHHAAAQGARTLRDLRHSLGVTAECGRCARC
                     AHECLRSAVPATPRPGVTALFAGIAPAFTASLEAT"
     gene            complement(5870..6064)
                     /locus_tag="PKV97_01060"
     CDS             complement(5870..6064)
                     /locus_tag="PKV97_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HNB04492.1"
                     /translation="MQPCEFPVSAAMPASRPDADSTAQRRPLLTSKELLGGRRELRIE
                     HEGSEYILRTTRNGKLILTK"
     gene            complement(6156..6563)
                     /locus_tag="PKV97_01065"
     CDS             complement(6156..6563)
                     /locus_tag="PKV97_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04493.1"
                     /translation="MLTLVLALWQAGAHAQGIVLVSAHAGGVAEVDRETAAQLYLGRR
                     TTLPDGRGVRLLDLPAGAERDLFYQRLTGKNPSQIRAYWSRMVFTGRAHPPHEAADAA
                     DALGRVLADPNALAYLPAASAANQPLNVLMLLE"
     gene            complement(6601..7803)
                     /locus_tag="PKV97_01070"
     CDS             complement(6601..7803)
                     /locus_tag="PKV97_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04494.1"
                     /translation="MTPRRTLLLLAGIAALASSPGTGAAPDLQVSGFATAGAVYSRAD
                     DLEFARVGIDAPGGNRVDTGPDSVLGVQLGWQSGDGTGAVLQVVTRETQRGDYTPRPA
                     LAFLSHALSPALTVRAGRLRSPFFMLSETADINYSQPWVRPPAEVYALNPFADMDGID
                     LLHRSPIGGSFIELHPYLGSSRIPVIGGGRANLRRLRGLTVTLERDELSFSASHAEAE
                     LAVIRTSQSYRDLVDTWRIPPDLQARLSGKGAHASFSSLGMQWDDGRWRVIAELGRLQ
                     ADAFVNSATGAYVAVGRRFGAWMPYFGLARQRQDAPLADPGLADSPQSAAAVAVFNRS
                     RNQAQRSVTLGTRWDFTPNSAVKAEFSHIETDRGAHGSFIARGNPFAPGLDDRSINLL
                     SVSVDVVF"
     gene            complement(7800..10265)
                     /locus_tag="PKV97_01075"
     CDS             complement(7800..10265)
                     /locus_tag="PKV97_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB04495.1"
                     /translation="MPPETSPSSFALRPSSIRIRLLLASTVVQVVLLSMLLANSVRLM
                     NNAMSASLETTIGQTASMLHAMATAYGEQGRLDGLEDVLGELLADADEGLLYVRIRDA
                     RGHLLVSAGMPGLEQVPEPDEQRHRFFETLLADDLVHVRRQLLLPRNQVGVLQFGVSV
                     SVLAAAREAIMQQGLAIAIAEILLTFVLLSSLGYLLTRNLRLLLEGSRAIANGFLAHR
                     VESRGKDELAVIANHFNLMAEALQNRIAELQDTAVRLRASEERHALALRGSNDGLWDW
                     DIDADDVYYSERFCQILGVQPGTLSNRPEDMLAYLHPYERDAYRQRLVEHLKGDSNHF
                     IMELRVRLPDGAYRWVLMRGVADRDENLHAYRMAGSLGDIDLRKRAEQQLVHDALHDG
                     LTGLPNRAMFIEHANRALAQRRRGDLTQIAILAINLERFSLVNDSYGHLVGDELLRRI
                     GAHIAALMRDGDVCARVGGDQFAVLLNGVESSIDALRICEQLVALPAFAPDDDGQQLH
                     PRCRVGVAISDNEREDAESLLRDADNALHKARRSERGPIEFFHTEMHAAAVRALQVEA
                     ELRKALTQGGLQVWYQPIVELANLRTTSFEALVRWPHPTLGLLSPGEFIPLAEALDLI
                     HDIGTLVLERVCADLREWAGRLPADAPLRVSVNLSARQLARPDLAAELLAAIDRVGVA
                     RERIRFEVTESVLAHPDGPAIDHLHALRAAGLDILIDDFGTGYSTLSYLHTMPCNQVK
                     LDGSFVRSLPYDQRLRAIVRHSIELAHDLGMTVVAECIEDEAQLEILRAMGCDFGQGY
                     LFSRPLDRDATFNRLHNPAPERR"
     gene            complement(10273..11655)
                     /locus_tag="PKV97_01080"
     CDS             complement(10273..11655)
                     /locus_tag="PKV97_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HNB04496.1"
                     /translation="MGGAGLVSRGLNRVRELVASAAHLGKPPDESDLERLRTQLQECA
                     EGRGGEVSTRQRAARLAETYLRLDDVGRHAFLRLIAHEFGPDPARVAQAHAAYQAAVG
                     TPRQWDAEAELRAALRSRRLRILTQFNAIPQGVKFLVDLRADLLRFIADDPLLKPLDR
                     ELETRLSAWFDVGFLELQRITWSSPAALLEKLVEYEAVHEIRSWKDLKNRLDSDRRCY
                     AFFHPRMPLEPLIFVQVALLEEIADDVQRLLDIEAPLADASRATTAIFYSISATQKGL
                     RGVSFGNFLLKRVIDDLKRDFPRLKTFATLSPMPGLVRWAQRDPEAFAEAFTATDWKR
                     LATLGIEGVDSTRLAAVLAGDPAWVEDEALAESLREPLLRLGARYLSEARRGDRPVDT
                     VARFHLGNGARIERLNWLADRSDKGLRQSWGLMVNYLYDPDRLEANLESFAANGSIAA
                     SSAVRRLARR"
     gene            complement(11777..12574)
                     /gene="lgt"
                     /locus_tag="PKV97_01085"
     CDS             complement(11777..12574)
                     /gene="lgt"
                     /locus_tag="PKV97_01085"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNB04497.1"
                     /translation="MLVHPQFDPIAFSLGPLSVRWYGLMYLVAFVLFVVLGRLHARRR
                     PELGWDEQGIDDLLMYGVIGVIVGGRLGEVLFFQPGYYLQHPGEILAVWKGGMSFHGG
                     FLGVLVAMWLYGRRHGKGFWQVTDFIAPLVPTGLAAGRLGNFINGELWGRPAETELPW
                     AMIFPWVDALPRHPSQLYQAAGEGLLLFLLLWSYSASPRPLRAVSAMFLVGYGALRFV
                     AEFFRTPDPGIFGTLSLGLSTAQWLCVPMVLAGLLMLAAARTRGTGR"
     assembly_gap    12590..12689
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12724..13401)
                     /locus_tag="PKV97_01090"
     CDS             complement(12724..13401)
                     /locus_tag="PKV97_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HNB04498.1"
                     /translation="MQDTTPTTEAGFDLDPAEIRVLGVLIEKSFITPDAYPLSINAIT
                     TGCNQLTAREPVMSLSEAEVQDAVDRLIARRLVSKRDQQSARVAKYEHLVRLRHSLPP
                     AEQAVLAILLLRGAQTAGELRQRCERLHRFDDVAAVDAVLEHLAEKYPPLVAELPRAP
                     GTKETRHVHLLGGDAAVQEMAEAQSVGTAGGGRGRIGELEDEVRRLREELEALRGAFA
                     EFRKQFD"
     gene            13550..15400
                     /gene="ilvD"
                     /locus_tag="PKV97_01095"
     CDS             13550..15400
                     /gene="ilvD"
                     /locus_tag="PKV97_01095"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNB04499.1"
                     /translation="MPQYRSRTSTAGRNMAGARALWRATGMKDGDFEKPIIAIANSFT
                     QFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMCNAHTADALVCISNCDKITPGMLMAALRLNIPAIFVSGGPMEAGKVKWEAKVI
                     SLDLVDAMVKAADKSCSDEEVDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GTTLATHADRERLFKEAGRRIVDLARRYYEKDDASVLPRSIASFQAFENAMSLDVAMG
                     GSTNTVLHLLAAAREAGVDFTMKDIDRVSRRVPCLCKVAPAIADVHIEDVHRAGGIMS
                     ILGELDRAGLLHTDVPTVHSASLAEALDKWDIKRTEDEAVHTFFRAAPGGVPTQVAFS
                     QDRRWKSLDADREHGIIRNKEHAFTADGGLAVLYGNIAEKGCIVKTAGVDESIWKFTG
                     KARVYESQEDAVEGILGEQVQAGDVVVIRYEGPKGGPGMQEMLYPTSYLKSRGLGAQC
                     ALLTDGRFSGGTSGLSIGHASPEAACGGAIALVEDGDTIEIDIPARRIHLAIADAELA
                     RRRAAMEAKGNAAWKPVKRERVVSAALQAYAALTTSADTGAVRDVTQVQR"
     gene            15451..15645
                     /locus_tag="PKV97_01100"
     CDS             15451..15645
                     /locus_tag="PKV97_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04500.1"
                     /translation="MDTELAQLEAQLEHLISHYERLKADNAALRGRVVQLEGENRRLS
                     DKVRVAADRLAGLLDKLPEA"
     gene            15650..15952
                     /locus_tag="PKV97_01105"
     CDS             15650..15952
                     /locus_tag="PKV97_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNB04501.1"
                     /translation="MSADHLDMMLLGKEYRISCTPESRDELLATVAFLDDKLREIGAR
                     TGSSGEKLAVMTALNIAHEFLLHQRGNGFDMPGMKRRIGLMKARLDGVLGQQDKLF"
     gene            15976..16159
                     /gene="ssrS"
                     /locus_tag="PKV97_01110"
     ncRNA           15976..16159
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKV97_01110"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            16338..17795
                     /locus_tag="PKV97_01115"
     CDS             16338..17795
                     /locus_tag="PKV97_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNB04502.1"
                     /translation="MQAYFPVLGVLGMMLMLFGLVMGFPLAVSFALEDGATEAYDLAI
                     LATFATGLLMWAAGRRARRDLHVSDGFLLVAATWLITPVFGALPLLFYLPELSFTDAY
                     FEAASGLTTTGATVLTGLDTMPVSINLWRCFMHWVGGMGVIVLMVAVLPLLGIGGRQI
                     FRAETPGPMKESSLTPRIAETAKGLWGVYVVLTAGCTLALSQAGMAPWDAVIHAFSIM
                     GLGGFSTRDASLGAFDSVPIETVTIVFALLSGINYATHYLALVRRTPRPYGEDPELRW
                     FFGVLGVTVVFLTVYLLESGAYRDGALAFRHVSFHAVSLATSLGLATYDYTVWPMFAQ
                     VWILFLSSFAACSGSTGGGIKMMRAIILYKQVHREIVRSLHPSAVVPLRLGRQPVSDN
                     VLHAVLAFSFMYMVSIVSLTLILAASGLDLITAFSGVVACINNAGPGLAALGPAGNYQ
                     GLGDFQTWVMSFAMILGRLEIFTLLVVLTPAFWRR"
     gene            complement(17752..18498)
                     /locus_tag="PKV97_01120"
     CDS             complement(17752..18498)
                     /locus_tag="PKV97_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB04503.1"
                     /translation="MKSAQPPSPVVLLVEDEAGIRRFVRAALESEGCTVREAAALERG
                     RIELASRRPDLLVLDLGLPDGDGTALLAELRGWSGMPVLVLSARSDEADKVAALDAGA
                     DDYLAKPFGVAELLARVRALLRRALRPPQATACIAFGDVELDLEQRRVTRGGAPVHLT
                     PIEFRLLGLLATAAGRVVTQRQLLAGAWGPNHVEHAHYLRVYMAGLRRKLEPDPRLPV
                     HILTEPGVGYRLVPEPLSAARTPASAPPAG"
     gene            complement(18495..19658)
                     /locus_tag="PKV97_01125"
     CDS             complement(18495..19658)
                     /locus_tag="PKV97_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNB04504.1"
                     /translation="MALPTPRDRLPDRAPLRNYLLAALAAGAVALVGAPLLGQFDLAN
                     IAMLFPLAVLFAAIRLGRGPAVFAAFLAVALFDFFFVHPHFTLAVSDLQYLLTFAVLL
                     AVALTTAELAARLRRERDSAEARAEEAAQARLAADTERLRNTLLASLSHDLRTPLTAL
                     AGLAESLPLAGPPLPPAQAELAEAIRAEALRTHALARDLLDLSRLQSGAPRLALDWLA
                     VDELLGSALQARAHALRAHVFALELPEELPLLHADAVLMERVVCNLLDNAIAHTPEGG
                     HIVLAAQVGAGWIRISLCDDGCGLPPGREQAIFERFVRAPHPRGAADATTATGDAGTG
                     LGLAIVHAIVAAHGGSVSAHNRAGGGACFVLQLPLPPQPDRPPPEDEAGAPPQ"
     gene            19817..20623
                     /locus_tag="PKV97_01130"
     CDS             19817..20623
                     /locus_tag="PKV97_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB04505.1"
                     /translation="MTFDLWWLAYPVLGAAVGFFAGLLGVGGGGIMVPMLTTFFLAQG
                     FPQSQVVHMALGTSMAAIVLTSVSSLRAHHARGAVRWDVVRAITPGILVGTFAATFIA
                     ARVSTVPLAIFFAVFMAYVSIQMLLNIKPKPARELPGALGMSAAGLGIGGVSALVAIG
                     GGSLSVPFMTWCNVKMQNAIGTSAAIGLPIALAGTVGYLVNGWGAPDMPALTFGYVYL
                     PALVLVSVVSTFFAPLGAKLAHRLPVATLKKVFAGMLVLLCAKMLHSIFA"
     gene            20658..21281
                     /gene="rhtB"
                     /locus_tag="PKV97_01135"
     CDS             20658..21281
                     /gene="rhtB"
                     /locus_tag="PKV97_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HNB04506.1"
                     /translation="MSWTMWLGFLAAALVIAVSPGPGAVLSMSTGLRYGYTAALRAIT
                     GLEIALLLQLGVVALGLGAVLATSETAFAVVKFAGAAYLIWLGVQKWRSPPEPIDGDG
                     EGVRVQSRRQLYAQGILVNLTNPKAIVFIAALVPQFIVPDRPQWPQFAIIGATMVGVD
                     VMVMSCYALLATRFRPLLRDPRLLRLQNRLFGGLFVGAGAMLAASSR"
     gene            complement(21456..22394)
                     /locus_tag="PKV97_01140"
     CDS             complement(21456..22394)
                     /locus_tag="PKV97_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNB04507.1"
                     /translation="MRFPYRLRRLRILAACAALLLGGSAHAQALEVATSFSILGDLVA
                     QVGGERIKVRTLVGPDEDAHAFQPRPSDAREIGKAALVVVNGLGFDDWMTRLARAGGF
                     KGAVVVASAGISTLEMRRDDGHDHGHDHGKGKAVDPHAWQDVANVRRYVANIAAALAT
                     ADPDGAATYRAAAARYDGELEALDAEIRAAFAALPAERRKVVSSHAAFGYFARAYDIR
                     FLSPVGVANNAEPTAKGVAGLIRQLAAEKVPAVFIENIADPRLIERIRSESGAVVGGT
                     LYSDALSKADGPAPSYVRMMRANLATLQKALAAPER"
     gene            complement(22641..23507)
                     /locus_tag="PKV97_01145"
     CDS             complement(22641..23507)
                     /locus_tag="PKV97_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNB04508.1"
                     /translation="MFDLLISPFTEFAFMRRALAGCLALALGATPVGVFLLLRRMSLM
                     GDAMSHAILPGAALGYLAFGLSLGAMTVGGIVAGLVVVFAAGLVTRASVLKEDASLAA
                     FYLLSLAAGVMIVSLRGQNLDLLHVLFGSVLGLDDASLLLIASIASVTLASLALLFRP
                     LVMECFDPAFLRGVSRWSPVAHYGFLMLVVVNLVSGFHALGTLMAVGIMILPAAAARL
                     WTNGLPMLLLLAVLFAFGSGASGLLLSFHADVPAGPAIVLVCGVVYFVSLLAAPGGLL
                     AGRLPVRHHLES"
     gene            complement(23543..24439)
                     /locus_tag="PKV97_01150"
     CDS             complement(23543..24439)
                     /locus_tag="PKV97_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB04509.1"
                     /translation="MNRLALPMIRLDNLTLGYDRHPAVHHLSAEIPAGALVAVVGPNG
                     AGKSTLLKGLAGELRPIGGRILLPELPAGGLAYMPQRGEIDHSFPVSVFDVVAMGLWH
                     EIGAFGGVSREQRRRVRAALEAVGMAGFESRPIGSLSGGQLQRARFARLMLQDAPLIL
                     LDEPFAAIDSRTVDDLVELILGWHDEGRTILTVVHDLEQVRRHFPTALLLSREPVAFG
                     PTAEVLTPERLARARRLSEAFDEHAHECHIEELTVAVAPDAAAHPHAHDHGHAHDEGH
                     GHAHPHPAAHTAPRPRRGLPRH"
     gene            complement(24436..24603)
                     /locus_tag="PKV97_01155"
     CDS             complement(24436..24603)
                     /locus_tag="PKV97_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04510.1"
                     /translation="MKRAPPVLHGTDPQADDDTRPFPARSLLGAGLAARLALVAGALA
                     LLWATVLWALQ"
     gene            complement(24600..25058)
                     /locus_tag="PKV97_01160"
     CDS             complement(24600..25058)
                     /locus_tag="PKV97_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNB04511.1"
                     /translation="MPLHEHPAATDAARELIAARGGRVTRTRVAVIQALQRSAHPLSH
                     DELGAALDGAGIAHDRVTLYRALDWLVEQGIAQRIAGGERAWRFEIRRDGDHRHAHFH
                     CERCGQIVCLEDVSAAAPTLPAGFAVARAELVLHGACADCGREGVERKER"
     gene            25228..>25786
                     /locus_tag="PKV97_01165"
     CDS             25228..>25786
                     /locus_tag="PKV97_01165"
                     /inference="COORDINATES: protein motif:HMM:NF022439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HNB04512.1"
                     /translation="MRPILLSSVIVLALALPVAAPAQHAHGAHQHGVAELTVVLEGRM
                     LQLELISPLDNLVGFEHAPANETQRGALADAERLLLDSEAVFALPPEAGCVIREVGIE
                     SPWRDSGREHSHGQTRDHNRPPRGDHEDMVASYGFECAHPEALRRLELRVFKHFPRLQ
                     RVRAEFATPRGQGGGVLTSGAAALAL"
BASE COUNT         3865 a         8705 c         9202 g         3914 t
ORIGIN      
        1 gcgggggcgg ggccggcccc tgcctggggc gggcgcgcct ggccctgggc gagtgcggcc
       61 atcgggatca gcagcgcatc cttggcctgc gcgaccacga agaagacctg tgcggtcatc
      121 tgcgtcatca gccggccgtc gggattgagg acgtcgaaga gcgcgttgta gagcaccacg
      181 ttgttggtca ccgtgggcgt gggctcgatc ttctcgagct tgccctgcca gcgcttgccg
      241 ctgccgccca gggtggtgaa gtaggcctcc atgccgagct tcaggcggcc gatgtcggct
      301 tccgatacct gggtctgcac ggtcatcgtg gacaggtcgg cgatgcgcat caccaccggt
      361 gcctgctggt tggtgttgag cgtctggccc tggcgcgcag tgatcgacac cacggtgccg
      421 gccatcggcg acaggatgcg ggcgtattgc aggttggcct cgtcggcgcg cagggtcgat
      481 tcgatctgct cgatctgcgc gcgcagcgcc tcgagctgag cctccgccga catcgccgag
      541 gcttcggcgg tctgcaggct ctccttggtg gtggcgtcct cggccatcag cgcgcgctgg
      601 cggcgctgct ggatctgcgc gagggcgagc tgggcctcgc ggtccttcag ctgcgcacgc
      661 aggttcttca actgggcgcg tgaggcgtcg acgcgggcac gatagaccgt ggggtcgatc
      721 tccgcgagca gctggccggc ctcgaccgtg tcgccgacct cgacgtggat cttgcgcaac
      781 tgaccggaga cctgcgcgcc cacgtccaca tagtcgcgcg gctgcagggt gccggtggcg
      841 gtgacgacgt cctcgacgtc gccgcgctgc acggtggtga actggtagtt ggtggtgggg
      901 tcgcgctcac cgatgaaggc gctgcgaccg taccaggcgc cggcgagcag cagcggcacc
      961 aggatcagca gggcgcggcg tccccagcgc gggcgggcgg ttcggttgga gggcttgtct
     1021 tcgttcattg ggctcagggc ttgccggcgg accgccgggc gctgcatcga ttcgtgggca
     1081 agagcatagc ggcccgcgag acctgcgctt gtatcggatt gaagccgcgt cgtttacggc
     1141 tggagagacc gcccgtggga cgcatcgttc cggcgcgcga ctccaaaacg ttgaccgtag
     1201 tcaagaaatg gcttgtttag acagagtcta attagcgcgt cggaacatcc tgctccgggc
     1261 agggtgggaa accaagaagc gacaattcgc cgaactggca aggagaagca tcatgatgaa
     1321 gttgaaggca acgagcgtgg cggtggcatt ggtggtggct ggcagcgcct gggcggccgt
     1381 ggcggtgcgg gacgaaccga tcaagccgat cgagccggcg aaggtggaga acgccgccaa
     1441 ggtcgagctc ggcaagaagt tgtggttcga gccgcgcctg tcgatgtcgg gcatcatctc
     1501 ctgcaatacc tgccacaacc tgtcgatggg gggcaccgac aacctcaaga cctcgatcgg
     1561 ccacggctgg aaagccggtc cggtgaactc gccgaccgtc tataactcca gcctggcgat
     1621 cgcccagttc tgggacggcc gtgcggccga cctcaaggag caggccggcg gcccgatcca
     1681 ggccggcgtc gagatgaaca tgccgcacac cctggcggtc ggcatcctgc agtcgatccc
     1741 gggctacgtc gaagagttcg agtcggtgta cggcaagaac aagatcgaca tcgacatgat
     1801 caccgactcg atcgccgagt tcgagaagac cctggtcacg cccgattcgc gcttcgacaa
     1861 gtggctcagg ggcgacgaca aggcgctgtc cgagaaagag ctcgccggct acaccctatt
     1921 caaggaaagc ggctgcgtgg cctgccacaa cggtccggcg gccggcggca cgtcattcca
     1981 gcgcatgggc gtggtcgagc cgtacaagag cagctcgccc gccgaaggcc gctcggcggt
     2041 caccggtgcc gatgccgacc gcttcaactt caaggtgccg accctgcgca acgtcgcgct
     2101 gacctacccg tacttccacg acggcgaggc ggcgacgctc gagcaggcgg tggataccat
     2161 ggggcgcctg cagctcggcc gcacctacag caaggatgag atcggacaga tcgtcgcctt
     2221 cctgaagacg ctgaccggcg aactgccgca gatcgcgatg ccggtgctgc cgccgtcgag
     2281 caacgcgacg ccgcgtcccg atccgttcaa gtaatccgaa aagccatccg gcttgtcgca
     2341 ggaatgccgg ggcacggacg caggttcgtg ccccggtact agttctcatg gattattgac
     2401 acgatccggg acccggtcca gcattcagac agggtccaat gtaaatccgc acagggtgcg
     2461 gatgcgcaac cagaaggtcg actgccatga tgaacaccta cgcatcctcc gacgccatcg
     2521 cctgcctgcg ccaggccggc attccggtca cgcaccagcg catcgagatt gcacgcgccc
     2581 tgttttcggc gccggtgcat ctctcggcgg accagatctg ggcgaaggtg cttgaggcct
     2641 cgcccgacgt ctcgcgcgcc acggtgtaca acaccttgcg gctgttcagc gagaagaaga
     2701 tgatccgcga gctgttcgtc gatccccagc gcgtggtgta cgactccacg accgcgccgc
     2761 accaccacct ctacaacatc gacagcggcg aggtgcgcga catctcggac gacgagttgc
     2821 gcgtgatcgg ccagccccgc ctgcccgcgg gcgtggtgct cgacgcaatc gacgtcatcg
     2881 tgcgcgtgcg caaccgcgcc gcctgacccc gcgtcccggg ggccgggacc gcggagtcgg
     2941 caatcaaaag aagcgcctgg gcgcggaacc gttccgcgcc caggcgcttt tgtccgtcca
     3001 ggcctcgacg cttagcgttc cggcacggtc acgaagccca tcttctcgat gccggcgctg
     3061 cgcgcctcgg ccatcacctc cgcgatcacc tcgtagcgcg tgttgcggtc ggcgcgcagg
     3121 tgaagctccg gctggggctt ttgcgccgcg gcggcgctca gacgggcggg aagggccacc
     3181 tcgtcgaccg cctcgttgtt ccagaacacg cggccgtcgc cgtcgatcga cagtgccacc
     3241 gtctccggct tctgttcggt cggcgcgctg gaggcctgcg gcaggtcgat cttgaccgag
     3301 tgggtcagca gcggcgcggt gatcatgaac acgatcagca gcaccagcat gacgtcgatc
     3361 agcggcacca tgttgatgtc gctctgcggg gcgcgcgtct cgccttggct gaatcccccg
     3421 aaggccatca cgccgctccg tgcgcgccgg ccggcgtgcc cgtggcgact tcggccgcgc
     3481 gtggcgcggc gccgggcact gcagcgatgc gtgcgccggt ggcgaaccag gcgtgcaggt
     3541 cgtgggcgaa ggcgtcgagt tcggagagct cgacgcggtt ggcgcgcgtg atgccgttgt
     3601 aggccagcac cgccgggatg gcgacgaaga ggccgaaggc ggtcatgatc agcgcctcgc
     3661 cgaccgggcc cgccaccttg tccagcgtcg ccatgcccga aacgctgatg ttcaccagcg
     3721 cgtggtagat gccccacacc gtgccgaaga ggccgacgaa gggggcggtc gagccgatcg
     3781 aggcgagcac ggtgaggccg gactcgagcg ccgcggtcga cagcgcgatc gacttgcgca
     3841 gcgcgcgggt gatgacctcg tcggtgtcga gcttgccgcc gagggtgttg ctgtgggtgt
     3901 gccggcgcac gtgctgggcg gcggcggcgc cctgctgggc aagcgcctcg aagggcgact
     3961 ccggggcgcg ctcggcgagc acgcgcagac cggcgtcgag gtcgggggcg gcccagaagg
     4021 cctctacggc gccgctgtgg ctgcgcgcac gcagctgacg cagggagcgg ctgaggatca
     4081 ggtaccagct ggtgatcgac atcaccagca ggatcagcgc caccgcgcgg atgatgttgt
     4141 cggattgcgc ccacaggtgg gcgaggccga aggcctgcga actgtccatg ctcagtttcc
     4201 ttgaagtttg aagacgatgg gcaccaggac ccaggattcg atcgcgctgt cgccgcgccg
     4261 tgcgggcacg aagcgccagc gcgccaccgc ctcgcgcgcg gcgcggtcga ggcgctcgga
     4321 gccggagctg gtgcgcagct cgacctgggc gggctggccg tcgggcgaca ccaacacgcg
     4381 cagcgtcacc tggccctcct cgcgcaggcg gcgcgacagc gccgggtagg ccggatgcgg
     4441 gttgttcaga taggcggcgt cgaagcgcgc ggcggtgacc gtggcgggcg cggccgtcgc
     4501 tgcgggtgct gcggcgggtg gtgccggcgc gggcggcgga ggggctgcgg gttcttcggc
     4561 cgcgctcagc gcggtgggcg actcggtgac tggcgccggc ttcttcacca cgggcttggg
     4621 cgtcggcttc ttgaccatcg gcggggttgg cggcgtgggc tcgggcgggc gcgccaccgg
     4681 ccgaggttca ggcgggcgcg gcggctccac gacgggttcg ggaggcgccg gtaagggctc
     4741 ggcgactgcg ggcgctgcga tgagtgcgac ctggattggc atcagctcgg cgggcacggg
     4801 gcgctcgccg gcgagcctgg cgagcatcgc cagcccgccg aggtgggcgg cgacgatcag
     4861 gccgaggagg gcgccgcgcc gcagcggcga gctcccgcgc gctgccgcgg tcgggcgcgg
     4921 agcgacttcc gctccggagg caggggcgcg catgggctgc gcctcaggat ccgtggacag
     4981 cgacggcgaa ggggaaggca acagcgacaa tgcttgggca gctgggtgca tcggggcgga
     5041 ggcgacgagc cgcctccgcg gaggcagcgt tgggttggtc gggagcgatc gggcgggccg
     5101 ggatcaggag gcgccggggt ccatctgcga ctgcaggtag ttctgcaggc cgacgctgtc
     5161 gaccagaccg agctgcgtct ccaggtagtc gatgtgttcc tcggtgtctt ccaggatgtg
     5221 ctcgaagagc tcgcgtgaca cgtaatcctt gctggactcg cagtaggcga tcgcctcgat
     5281 caggttggcg cgtccgccga gctcgagctt gagatccccc tgcatgcatt cgggcacgtt
     5341 ctcgccgatc agcagcttgt cgagattctg caggttgggc agcccctcca ggaagagcac
     5401 gcgctcgatc agcatgtccg cgtgcttcat ctcctcgatc gactcgtcgt actcgtgctt
     5461 gccgagccgc gtgaaacccc agttcttgta catgcgcgca tgcaggaagt actggttgat
     5521 cgcggtgagt tcgatggtga gctgcttgtt gaggtactgg atgactttct tgtcgccttt
     5581 catgttgcct ccagggatgc ggtgaatgcc ggagcgatgc ctgcgaacag cgcggtcacg
     5641 cccgggcggg gcgtggcggg tacggccgag cgcaggcact cgtgcgcgca gcgggcgcag
     5701 cgaccacact ctgcggtcac gccgagcgaa tggcgcaggt cgcgcagggt ccgcgcgccc
     5761 tgcgccgcgg cgtggtggat ctggcgttcg gtgaccgcgt tgcatacgca cacgtacata
     5821 gtctagcctc ctgacggggt tttgccccga tatggcatcg cctggaaggt catttggtga
     5881 ggatcagctt gccgttgcgc gtcgtgcgca agatgtactc gctgccctcg tgctcgatcc
     5941 gcaactcgcg ccggccgccg aggagctcct tgctggtgag cagggggcgc cgctgtgcag
     6001 tcgaatccgc atcgggccgg ctggccggca tggcggcgga aacggggaat tcgcaaggtt
     6061 gcatgatcga acgacgttaa aaatacgaat gattcgcatt aaatcacgag tcgccgacgc
     6121 atgcaatgcc ggcgcgcatt cgctgcccgc tggcttcact ccaggagcat caggacgttc
     6181 aggggctggt tggcggcaga ggcggcaggg aggtaggcga gcgcgttcgg gtcggcgagc
     6241 acgcggccga gcgcgtcggc tgcgtcggcc gcctcgtggg gcggatgggc gcgcccggtg
     6301 aagaccatcc gcgaccagta ggcgcggatc tggctggggt tcttgccggt caggcgctga
     6361 tagaagaggt cgcgttcggc gccggccggc aggtcgagca ggcggacgcc acgcccgtcg
     6421 ggcagggtgg tgcggcggcc gaggtagagc tgggcggcgg tctcgcggtc gacttcggcg
     6481 acgcctccgg cgtgggcgct gaccaggacg atgccttgcg cgtgcgctcc ggcctgccag
     6541 agggcgagca cgagcgtcag catcggcgcg agaccgagcc gtcggagcag gcgcgccgga
     6601 tcagaacacc acatcgaccg acacgctcag cagattgatg ctgcgatcgt cgagaccggg
     6661 ggcgaagggg tttccccgcg cgatgaagct gccgtgcgcg ccgcgatcgg tttcgatgtg
     6721 gctgaattcc gccttcaccg ccgaattcgg cgtgaagtcc caacgcgtgc cgagggtgac
     6781 gctgcgctgg gcctggttgc gcgagcggtt gaagaccgca accgctgcgg cgctttgtgg
     6841 cgagtccgcc agcccggggt cggccagcgg cgcgtcctgg cgctggcggg cgagaccgaa
     6901 ataaggcatc catgcgccga agcgccgccc gaccgcgaca taggctccgg tcgcgctgtt
     6961 gacgaaggcg tccgcctgca ggcgcccgag ctcggcgatc acccgccagc gcccgtcgtc
     7021 ccactgcatg ccgagggagc tgaagctggc gtgggcaccc ttcccgctca atcgcgcctg
     7081 caggtcgggc gggatccgcc aggtgtcgac gaggtcccgg tacgactgcg aggtccggat
     7141 caccgccagc tccgcctccg cgtggctcgc gctgaagctc aactcgtcgc gctccagcgt
     7201 cacggtgagg ccgcgcagcc ggcgcagatt cgcgcgtccg ccaccgatca cggggatgcg
     7261 gctgctgcca agatacgggt gcagctcgat gaaggatccg ccgatcgggc tgcgatgcag
     7321 caggtcgatg ccatccatgt cggcgaaggg gttgagcgcg tagacctctg ccggcgggcg
     7381 gacccagggc tggctgtagt tgatgtcggc ggtctcggac agcatgaaga aggggctgcg
     7441 caggcgcccg gcacgcacgg tcagcgcggg gctcagggcg tggctcagga aggccagggc
     7501 aggccgcggc gtgtagtcgc cgcgctgggt ctcgcgcgtc acgacctgca gcaccgctcc
     7561 ggtgccgtca ccggactgcc agccgagctg taccccgagc accgagtccg gcccggtgtc
     7621 gacgcggttg ccgcccggcg cgtcgatccc gacgcgggcg aactccaggt cgtccgcgcg
     7681 gctgtagacc gccccggcgg tcgcgaagcc cgacacctgc aggtcgggtg cggcgcccgt
     7741 gccggggctg gacgccagcg cggcgatgcc ggcgagcagc aacaaggtgc ggcgaggggt
     7801 catcggcgct ccggagcggg attgtgcaag cggttgaagg tggcgtcgcg gtcgagcggc
     7861 cgcgagaaca ggtagccctg tccgaagtcg cagcccatcg cgcgcaggat ctcgagctgg
     7921 gcctcatcct cgatgcactc ggcgacgacg gtcatgccga ggtcgtgggc gagttcgatg
     7981 ctgtgccgca cgatcgcgcg caggcgctgg tcgtacggca gcgagcgcac gaaggagccg
     8041 tcgagcttga cctggttgca cggcatcgtg tgcaggtagc tcagcgtcga gtagccggtg
     8101 ccgaagtcgt cgatgaggat gtcgagtccg gcggcgcgca gggcatggag gtggtcgatc
     8161 gccgggccgt ccggatgggc gagcacgctc tccgtgacct cgaagcggat gcgttcgcgc
     8221 gccacgccga cacggtcgat ggcggcgagc agttcggcgg cgagatccgg gcgtgcgagc
     8281 tggcgcgcgg acaggttcac gctgacgcgc agcggcgcgt cggctggcag ccggcccgcc
     8341 cattcgcgca ggtcggcgca cacgcgctcg agcacgagcg tgccgatgtc gtggatcagg
     8401 tcgagcgcct cggcgagcgg gatgaactcg cccggcgaga gcaggccgag cgtcgggtgc
     8461 ggccagcgca ccagggcctc gaagctggtc gtacgcaggt tcgcgagctc gacgatgggc
     8521 tggtaccaca cctgcagccc gccctgggtg agcgccttgc gcagttcggc ctcgacctgc
     8581 agcgcgcgca ccgcggcggc gtgcatctcg gtgtggaaga attcgatcgg cccccgctcg
     8641 ctgcgacggg ccttgtgcag tgcgttgtcg gcatcgcgca gcagcgattc ggcgtcctcg
     8701 cgctcgttgt cgctgatggc gacgcccacc cggcagcgcg ggtgcagttg ctgaccgtcg
     8761 tcgtcgggtg cgaaggccgg cagcgcgacc agctgctcgc agatgcgcag cgcgtcgatc
     8821 gagctctcca caccgttgag cagcaccgcg aactggtccc cccccacccg cgcgcacacg
     8881 tccccatcgc gcatcagggc cgcgatgtgg gcaccgatcc ggcgcagcag ttcgtcgccc
     8941 acgaggtgcc cgtagctgtc gttgaccagg ctgaagcgct ccaggttgat cgccaggatc
     9001 gcgatctggg tgaggtcccc gcgccggcgc tgggcaagcg cgcggttggc gtgctcgatg
     9061 aacattgccc ggttgggcag gccggtgagc ccgtcgtgca aggcatcgtg caccagctgc
     9121 tgttcggcgc gcttgcgcag gtcgatgtcg ccgagcgaac ccgccatgcg gtaggcatgc
     9181 aggttctcgt cgcgatcggc gactccgcgc atcagcaccc agcggtaggc gccgtcgggc
     9241 aggcggacgc gcagttccat gatgaaatgg ttgctgtcgc ccttgaggtg ttcgaccagg
     9301 cgctggcggt aggcgtcgcg ctcgtagggg tggaggtagg cgagcatgtc ctccggccgg
     9361 ttcgacaggg ttcccggctg gaccccgagg atctggcaga agcgctcgga gtaatagacg
     9421 tcgtcggcgt cgatgtccca gtcccacagg ccgtcgttgg acccgcgcag ggccagggca
     9481 tggcgctcct cgctcgcccg caggcgcacc gccgtatcct gcagctcggc gatccggttc
     9541 tgcagcgcct cggccatcag gttgaagtgg ttggcgatca ccgccagctc gtccttgcca
     9601 cgcgactcga cgcggtgcgc gaggaagccg ttggcgatcg cacgactgcc ctccagcagc
     9661 aggcgcaggt tgcgggtgag cagatagccg agcgaggaga gcaggacgaa ggtgagcagg
     9721 atctccgcga tcgcgatcgc caggccttgc tgcatgatcg cctcgcgggc cgcggcgagt
     9781 accgagaccg agactccgaa ctgcagcacg ccgacctggt tgcgcggcag cagcagttgc
     9841 cggcgcacat gcacgaggtc gtcggcgagc agggtctcga agaaacggtg gcgttgttcg
     9901 tccggctccg gcacctgctc cagcccgggc atgccggcgc tcaccagcag atgaccgcgc
     9961 gcgtcgcgga tgcgcacata gagcaggcct tcgtcggcgt cggcaagaag ctcgccgagc
    10021 acgtcctcga gcccgtcgag ccggccctgc tcgccgtagg cggtggccat cgcgtgcagc
    10081 atgctcgcgg tctgaccgat cgtcgtctcc aggctcgccg acattgcgtt gttcatcagc
    10141 cgcacgctgt tcgcgagcaa catgctcagc aacaccacct gcaccaccgt actcgcgagc
    10201 aacaggcgga tgcggatcga cgaggggcgg agggcgaacg agctcggcga tgtttcgggc
    10261 ggcacgcgaa cctcagcgcc gggcgagcct gcgcaccgcg gacgaggctg cgatgctgcc
    10321 gttggcagcg aagctctcca ggttggcttc caggcggtcc gggtcgtaca gataattgac
    10381 catcaatccc caagactggc gcaggccctt gtccgagcgg tcggcgagcc agttcaggcg
    10441 ctcgatgcgg gcaccgttgc cgaggtggaa gcgcgccacc gtgtcgaccg ggcggtcgcc
    10501 gcggcgtgcc tcgctcaggt agcgtgcgcc cagccgcagc aggggctcgc gcaaggactc
    10561 ggcgagcgcc tcgtcctcga cccacgccgg gtcgccggcg agcaccgcgg cgaggcgcgt
    10621 cgagtcgacg ccttcgatgc cgagcgtcgc caggcgcttc cagtcggtgg cggtgaaggc
    10681 ctcggcgaag gcctccgggt cgcgctgcgc ccagcgcacc aggcccggca tcggcgacag
    10741 cgtggcgaag gtcttcaggc gcggaaagtc gcgcttgagg tcgtcgatca cccgcttgag
    10801 caggaagttg ccgaagctca ccccgcgcag gcccttttgc gtggcgctga tcgaatagaa
    10861 gatcgcggtc gtcgcccggc tcgcatcggc gagcggcgcc tcgatgtcga gcaggcgctg
    10921 cacgtcgtcg gcgatctcct cgagcagtgc gacctgaacg aagatcagcg gctcgagcgg
    10981 catgcgcgga tggaagaagg cgtagcagcg gcggtcggag tcgagccggt tcttgaggtc
    11041 cttccaggag cggatctcgt gcaccgcctc gtactcgacg agcttctcga gcagggcggc
    11101 gggcgaactc caggtgatgc gctgcagttc caggaagccg acgtcgaacc acgccgacag
    11161 gcgcgtctcg agctcgcggt cgagcggctt gagcagcgga tcgtcggcga tgaagcgcag
    11221 caggtcggca cgcaggtcga cgaggaactt gaccccctgc gggatcgcgt tgaactgggt
    11281 gaggatgcgc agccggcgcg agcgcagcgc ggcgcgcagc tcggcttccg cgtcccattg
    11341 ccgcggcgtg ccgaccgcgg cctggtaggc ggcgtgcgcc tgcgccaccc gcgccggatc
    11401 ggggccgaac tcgtgggcga tcaggcgcag gaaggcgtgg cggccgacgt cgtccaggcg
    11461 cagataggtc tcggccaggc gggcggcacg ctggcgcgtc gatacttcgc cgccgcggcc
    11521 ctcggcgcac tcctgcaact gcgtgcgcag gcgctcgaga tcgctctcat ccgggggctt
    11581 gcccaggtgc gcggcgctcg cgacgagctc gcgaacgcgg ttcagtcccc gggagaccag
    11641 tcctgcaccg cccatgtcgt cctcctgtcc gattcagttc ggatatcgtg cttacatgat
    11701 agcccgcttt ggggagtcgc ttgtgcagcc tgcgaatgga ctgtgtgcga tattcacgtc
    11761 cctgccgcag ccccgcctac cttccggtcc cgcgcgtgcg cgcggcggcg agcatcagca
    11821 gcccggcgag caccatgggc acgcaaagcc attgtgcggt ggacaggccg agcgacaagg
    11881 tgccgaagat gcccggatcg ggcgtacgga agaactccgc gacgaagcgc agcgcgccat
    11941 agccgaccag gaacatcgcc gacaccgcgc gcagcgggcg cgggctggcc gagtaagacc
    12001 acagcagcag gaacagcagc aaaccctcgc cagcggcctg gtagagctgc gagggatggc
    12061 gcggcagcgc atccacccag gggaagatca tggcccacgg cagctccgtc tccgccggcc
    12121 ggccccacag ttcgccgttg atgaagttgc cgagccgccc ggccgccagc ccggtcggca
    12181 ccagcggcgc gatgaagtcg gtgacctgcc agaagccctt gccgtgccgg cgaccgtaca
    12241 gccacatcgc caccagcacg cccaggaagc cgccgtggaa gctcatcccg cccttccaca
    12301 ccgcgaggat ctcgcccggg tgttgcagat agtagccggg ctggaagaac aacacctcgc
    12361 cgaggcgccc gcccacgatc accccgatca caccgtacat caggaggtcg tcgatcccct
    12421 gctcgtccca gcccagctcc ggacggcgcc gcgcgtggag gcggccgagc acgacgaaga
    12481 gcacgaaggc gaccaagtac atcagcccgt accagcgcac cgaaagcggg ccgagggaga
    12541 aggcgatcgg gtcgaattgg gggtggacga gcatggagtg tgcgaggcgn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ggtgtgaggc gccgagcgca gggctgccgg
    12721 ccttcagtcg aactgcttgc ggaactccgc gaacgctccg cgcagtgcct cgagttcctc
    12781 gcgcagacgg cggacctcgt cctcgagttc cccgatgcgc ccgcgcccgc cgcctgccgt
    12841 gccgacggac tgcgcctcgg ccatctcctg caccgcggcg tcgccgccga gcaggtgaac
    12901 gtggcgggtc tccttggtgc cgggcgcacg cggcagctcg gcgaccagtg gcgggtactt
    12961 ctcggccagg tgctcgagca ccgcatccac cgcggcgacg tcgtcgaaac ggtgcaggcg
    13021 ctcgcagcgc tggcgcagct cgcccgcggt ctgcgcgccg cgcagcagca ggatcgccaa
    13081 tacggcctgt tcggccggcg gcaacgagtg gcgcaggcgg accaggtgct cgtacttggc
    13141 cacgcgcgcg ctctgctggt cgcgcttgga cactaggcgg cgggcgatca ggcggtcgac
    13201 cgcgtcctgc acctcggcct cggacaggct catcaccggc tcgcgcgcgg tgagctggtt
    13261 gcatccggtg gtgatggcgt tgatcgacag cggataggcg tcgggggtga tgaaggactt
    13321 ctcgatcagc acgccgagca cgcggatctc ggccgggtcg aggtcgaagc cggcctcggt
    13381 cgtgggggtc gtgtcctgca tggagcctcc tgcggtgtcg gacttcggat gatacgccag
    13441 ccggatcgtc cgccccaccg cggcgtcccc gcatgcaccg cagcttgccg acgctcgccc
    13501 cacgcgcgtt agaatcgaac cctttcccgc cgatttcccg gagcgcccca tgccccagta
    13561 ccgttcccgc acgtccaccg ccggccgcaa catggcgggc gcccgcgccc tgtggcgcgc
    13621 caccggcatg aaggacggcg acttcgaaaa gccgatcatc gcgatcgcca acagcttcac
    13681 ccagttcgtg cccggccacg tgcacctgaa ggatctcggt cagctcgtcg cgcgcgagat
    13741 cgaggccgcc ggcggcgtcg ccaaggaatt caacaccatc gcggtcgatg acgggatcgc
    13801 catgggccac ggcggcatgc tgtattcgct gccctcgcgc gagctcatcg ccgacagcgt
    13861 cgagtacatg tgcaacgcgc acacggcgga cgcgctggtg tgcatctcga actgcgacaa
    13921 gatcaccccg ggcatgctga tggccgcgct gcgcctgaac atcccggcga tcttcgtctc
    13981 cggcggtccg atggaggccg gcaaggtcaa gtgggaagcc aaggtgatct cgctcgacct
    14041 cgtggatgcg atggtcaagg cggccgacaa gtcgtgttcg gacgaggaag tcgacgccat
    14101 cgagcgctcg gcctgcccga cctgcgggtc gtgctcgggc atgttcacag ccaactcgat
    14161 gaactgcctc accgaggcgc tcggcctgtc gctgcccggc aacggcacca cgctcgccac
    14221 ccacgccgac cgcgagcgcc tgttcaagga agccggccgc cgcatcgtcg atctcgcgcg
    14281 tcgttactac gaaaaggacg acgcctcggt gctgccgcgc tcgatcgcca gcttccaggc
    14341 cttcgagaac gcgatgagcc tggacgtggc catgggcggc tcgaccaaca ccgtgctgca
    14401 cctgctcgcc gccgcgcgcg aggccggcgt ggactttacg atgaaggaca tcgaccgcgt
    14461 cagccgccgc gtgccctgcc tgtgcaaggt cgcgcccgcg atcgccgacg tgcacatcga
    14521 ggacgtgcat cgtgccggcg gcatcatgtc catcctcggt gaactcgacc gcgccggcct
    14581 gctgcacacc gatgtgccga ccgtgcacag cgcgagcctg gccgaagccc tggacaagtg
    14641 ggacatcaag cgcaccgaag acgaagcggt gcacaccttc ttccgtgcag cgccgggcgg
    14701 ggtgccgacc caggtcgcct tcagtcagga ccggcgctgg aagtcgctcg acgccgaccg
    14761 cgagcacggc atcatccgca acaaggaaca tgccttcacg gccgacggag ggctggccgt
    14821 gctctacggc aacatcgccg agaagggctg catcgtgaag accgccgggg tggatgaatc
    14881 catctggaag ttcaccggca aggccagggt gtacgagagc caggaagacg cggtggaggg
    14941 catcctcggc gagcaggtgc aggcgggcga cgtggtggtg atccgctacg aaggcccgaa
    15001 gggcggcccc ggcatgcagg agatgctcta tcccacctct tacctgaaga gccgcggcct
    15061 gggcgcgcag tgcgcgctgc tcaccgacgg acgcttctcg ggcggcacct cgggcctgtc
    15121 gatcggccat gcgtcgcccg aggcggcctg cggcggcgcg atcgcgctgg tcgaggacgg
    15181 cgacacgatc gagatcgaca tccccgcgcg ccgcatccat cttgccatcg ccgacgccga
    15241 gctcgcccgc cggcgtgccg cgatggaggc gaagggcaat gcggcctgga agccggtgaa
    15301 gcgcgaacgc gtcgtctccg ccgcgctgca ggcctacgcc gcgctcacca cctcggccga
    15361 caccggcgcg gtgcgggacg tcacccaggt ccagcgctga tcgaccccgg ccgattgacc
    15421 cgtcatcggc cgctcactac catcgacgcc atggataccg aactcgcaca gctcgaagcc
    15481 cagctcgagc acctgatcag ccactacgag cgcctgaagg ccgacaacgc cgccctgcgc
    15541 ggtcgcgtcg tgcagctcga gggcgagaat cgccggctct ccgacaaggt ccgcgtcgcc
    15601 gcggaccgcc tcgcgggcct gctcgacaaa ctgccggagg cttgaagcga tgtccgccga
    15661 tcacctcgac atgatgctgc tgggcaagga gtaccgcatc tcctgcacgc ccgaatcgcg
    15721 cgacgaactg ctcgccaccg tcgccttcct cgacgacaag cttcgtgaga tcggtgcccg
    15781 caccggctcc tccggcgaaa agctggcggt catgaccgcg ctcaacatcg cccacgagtt
    15841 cctcctgcac cagcgcggca acgggtttga catgccgggc atgaagcgta gaatcggtct
    15901 catgaaagcg cgcctggacg gtgttctggg gcagcaggac aagcttttct gaaggcagtt
    15961 cgagcagtac cggtcaatcc cctgcagtgt tcgtcgaagt cgtagattcc ttgaaccaat
    16021 agtcgagaga ctcggtcgcc aaccatcgat gccgctggtg tgaacgcccc tcgccgggtg
    16081 atcctgaagc ggaagggacg cgacctccct gaacccaagg ttcaggatgc ccggcggaga
    16141 cggcacaggc ggggggcctt acaagagggc gcggtgagcg atcaccgcgc cctcttcacg
    16201 tccacgcgcc cgacacctcg ctccctgcgc ctcacgctca tgctgcactg caaactctta
    16261 acgcaatctt tacgcatggc cgcctagtat ccgcagcgtc catacacagt ctcgcggcgt
    16321 ttgccgtcct tgtcgcgatg caagcctatt tccccgtcct cggtgtgctc ggcatgatgc
    16381 tgatgctgtt cgggctggtg atgggctttc cgctcgcggt gtccttcgcg ctcgaagacg
    16441 gtgccaccga ggcctacgac ctcgccatcc tcgccacctt cgcgaccggg ctgctcatgt
    16501 gggccgcggg ccggcgggcg cggcgcgacc tgcacgtctc cgacggcttc ctgctcgtgg
    16561 ccgcgacctg gctgatcacg ccggtgttcg gggcgctgcc gctgctgttc taccttccgg
    16621 aactctcgtt caccgacgcc tacttcgagg ccgcctccgg gctcaccacg accggtgcca
    16681 cggtgcttac cgggctcgac acgatgccgg tgtcgatcaa cctgtggcgc tgcttcatgc
    16741 actgggtggg cggcatgggc gtgatcgtgc tgatggtcgc ggtgctgccg ctgctgggca
    16801 tcggcgggcg gcagatcttc cgcgccgaga cgcccgggcc gatgaaggag tccagcctca
    16861 ccccgcgcat cgccgagacc gccaagggcc tgtggggcgt ttatgtagtg ctcaccgcag
    16921 gctgcaccct cgcgctctcg caggccggca tggcgccgtg ggatgccgtg atccatgcct
    16981 tctcgatcat ggggctgggc ggcttctcca cccgggacgc cagcctcggc gccttcgaca
    17041 gcgtgccgat cgagaccgtc accatcgtgt tcgcgctgct ctcgggcatc aactatgcca
    17101 cccactacct cgccctggtg cggcgcacgc cgcgccctta cggcgaggac cccgagctac
    17161 gctggttctt cggcgtgctc ggggtcaccg tggtctttct gaccgtgtat ctgctcgaat
    17221 cgggcgccta tcgggatggc gcgctcgcgt tccgccacgt ctccttccac gccgtctcgc
    17281 tggcgacctc gctcggcctg gcgacctacg actacacggt gtggccgatg ttcgcgcagg
    17341 tgtggatcct gttcctgagc agcttcgccg cctgctccgg ctcgaccggt ggcggcatca
    17401 agatgatgcg cgcgatcatc ctgtacaagc aggttcaccg cgagatcgtg cgctcgctgc
    17461 atccgagcgc ggtggtgccg ctgcgtctgg gcaggcagcc ggtgtccgac aacgtgctgc
    17521 acgcggtact cgcgttcagc ttcatgtaca tggtcagcat cgtcagcctg acgctgatcc
    17581 tcgccgcttc cgggctggac ctgatcaccg cgttctcggg cgtggtggcc tgcatcaaca
    17641 atgccgggcc agggctggcc gcgctggggc cggcgggcaa ctaccagggc ttgggcgatt
    17701 tccagacttg ggtgatgagc tttgcgatga ttctcggccg cctggagatc ttcaccctgc
    17761 tggtggtgct gacgccggcg ttctggcggc gctgagcggc tcgggcacca gccggtagcc
    17821 cacgccgggt tcggtgagga tgtggacggg caggcgcggg tcgggttcga gcttgcggcg
    17881 caaaccggcc atatagacgc gcaggtaatg tgcgtgctcg acgtggttcg ggccccaggc
    17941 gccggccagc agctggcgct gcgtcaccac gcgacccgct gcggtggcga gcaggccgag
    18001 caggcggaac tcgatcgggg tcaggtggac cggcgcgccg ccacgcgtca cccggcgctg
    18061 ctcgaggtcg agttccacgt cgccgaaggc gatgcacgcc gtggcctgcg gcggacgcag
    18121 cgcgcggcgc agcagggcgc ggacgcgggc gagcaactcg gcgacgccga agggcttggc
    18181 gagatagtcg tcggcgccgg cgtcgagcgc cgcgaccttg tcggcctcgt cgctgcgcgc
    18241 cgacagcacc agcaccggca tgccgctcca tccgcgcagc tcggccagca gcgccgtgcc
    18301 gtcgccgtcg ggcaggccga ggtcgagcac cagcaggtcg ggccggcggc tggcgagctc
    18361 gatgcggccg cgctcgagcg cggcggcctc gcgcaccgtg cagccctcgg attccagcgc
    18421 cgcgcgcacg aaacggcgga tgccggcctc gtcctcgacc agcagcacga ccggggaggg
    18481 gggctgggcg ctcttcattg tgggggcgct ccggcctcgt cctcgggagg cggtcggtcc
    18541 ggctggggtg gcagcggcag ctgcagcacg aagcaggcgc cgccgccggc gcggttgtgt
    18601 gcgctcacgc tgccgccgtg tgccgcgacg atggcgtgca cgatcgccag acccagcccg
    18661 gtgccggcat cgccggtcgc cgtcgttgcg tcggccgcgc cgcgcggatg gggggcgcgc
    18721 acgaagcgct cgaagatcgc ctgctcgcgc cccggcggca ggccgcaacc gtcgtcgcac
    18781 agactgatgc ggatccagcc ggcgccgacc tgcgccgcca gcacgatgtg ccctccctcc
    18841 ggggtgtgcg cgatcgcgtt gtcgagcagg ttgcacacca cccgctccat cagcaccgcg
    18901 tcggcgtgca gcagtggcag ttcctcgggc agctcgagcg cgaacacgtg cgcgcgcagg
    18961 gcgtgggcgc gcgcctgcag cgcgctgccg agcagctcgt ccaccgccag ccagtcgagc
    19021 gcgaggcgcg gcgccccgga ttgcaggcgg gagaggtcga gcaggtcgcg cgcgagcgca
    19081 tgggtgcgca aggcctcggc gcggatcgcc tcggccagct cggcctgcgc cgggggcagc
    19141 ggcggtccgg ccagcgggag cgactcggcg agtccggcga gtgcggtcag cggtgtgcgc
    19201 aggtcgtgcg acagcgaggc gagcaaggtg ttgcgcaggc gctcggtgtc ggcggcgagg
    19261 cgcgcctgtg ccgcctcttc cgcacgcgcc tcggcagagt cgcgttcgcg gcgcaggcgc
    19321 gcggccagct ccgcggtggt cagtgccacc gcgagcagca cggcgaaggt cagcaggtac
    19381 tgcaggtccg agaccgccag cgtgaagtgc ggatgcacga agaagaagtc gaacagcgcc
    19441 accgccagga aggcggcgaa cacggccggt ccgcgaccga gccggatcgc tgcgaacagc
    19501 accgcgagcg ggaacagcat ggcgatgttg gcgagatcga actgcccgag cagcggcgca
    19561 ccgaccagcg ccaccgcacc cgccgccagc gcggcgagca ggtaattgcg cagcggggcg
    19621 cggtcgggaa ggcggtcgcg tggtgtcggc agggccatgc cgcgatggta ccagcgtgcc
    19681 catcccgccg cgtccgcgcc ggccctgccc gggaggattc ccacggtccg cttgcactgc
    19741 ccctcggctt tgaccgatag ttccgctcct tgcgcactgc aacacgcggc gtgcctcgaa
    19801 cccgaacgga aacgcaatga ccttcgacct ctggtggctc gcctacccgg tgctcggcgc
    19861 ggcggtgggc ttcttcgccg gcctgctcgg tgtgggcggc ggcggcatca tggtgccgat
    19921 gttgacgacc ttcttccttg cccagggctt cccgcagtcc caggtggtgc acatggcgct
    19981 cggcacctcg atggcggcca tcgtgctgac ctcggtgtcc agcctgcgtg cgcaccacgc
    20041 gcgtggcgcg gtgcgctggg acgtggtgcg cgcgatcacg cccggcatcc tcgtcggcac
    20101 cttcgccgcc actttcatcg ccgcgcgcgt gtccaccgtg ccgctggcga tcttcttcgc
    20161 cgtcttcatg gcctacgtgt cgatccagat gctgctcaac atcaagccca agcccgcgcg
    20221 cgagctgccc ggggcactcg gcatgagcgc ggccggcctc ggcatcggcg gtgtgtcggc
    20281 gctggtcgcg atcggcggcg gctcgctgtc ggtgcccttc atgacctggt gcaacgtcaa
    20341 gatgcagaac gcgatcggta cctccgcggc gatcggcttg ccgatcgcgc tcgccggcac
    20401 ggtcggctac ctggtcaatg gctggggcgc acccgacatg ccggcgctca ccttcggcta
    20461 tgtgtacctg ccggcgctgg tgctggtctc cgtggtcagc accttcttcg ccccgctcgg
    20521 cgcgaagctg gcccaccgcc tgccggtggc gacgctgaag aaggtgtttg ccgggatgct
    20581 ggtcctgctg tgtgccaaga tgctgcacag catcttcgcc tgaacgaact cgctggacga
    20641 atctgccgga gacttccatg agctggacga tgtggcttgg ctttcttgcc gccgcactgg
    20701 tgatcgcggt ctcgccggga ccgggcgccg tgctgtcgat gtcgaccggc ctgcgctacg
    20761 gctacaccgc cgcgctgcgc gcgatcaccg gcctggagat cgcgctgttg ctgcaactcg
    20821 gcgtcgtggc gctcggcctc ggtgcggtgc tcgcgacctc ggagacggcg ttcgcggtgg
    20881 tgaaattcgc cggcgcggcc tacctgatct ggctcggcgt gcagaagtgg cgctcgccgc
    20941 ccgagccgat cgacggggac ggcgagggcg tgcgggtgca gtcgcgccgc cagctctacg
    21001 ctcagggcat cctggtgaac ctgaccaacc cgaaggcgat cgtgttcatc gccgcgctgg
    21061 tgccgcagtt catcgtgccc gaccggccgc agtggccgca gttcgcgatc atcggcgcaa
    21121 ccatggtcgg ggtcgacgtg atggtgatgt cctgctacgc gctgctcgcc acgcgtttcc
    21181 gccccctgct gcgcgacccg cgcctgctgc gcctgcagaa ccgcctgttc ggcggcctct
    21241 tcgtcggcgc cggggcgatg ctggcggcct cgagccgctg agccgccccg ggtccgggcc
    21301 ggcaatgggc gatggcgcga acggcggcgc cgtaatcgcg gcttgcgtcg ctcccacaag
    21361 aacgctccgc cgcccccgag ccccggcagg agcgacgcaa gtcgcgatcc cccgcgcccc
    21421 gagacccgcc gccgctccgt ggaaaccgat ccggttcagc gctcgggtgc cgcgagtgcc
    21481 ttctgcagcg tcgccaggtt ggcgcgcatc atgcgtacgt agctgggcgc cgggccgtcg
    21541 gccttcgaca gtgcgtcgga atacagcgtg ccgcccacga ccgcgccgct ttcgctgcgg
    21601 atgcgctcga tcaggcgagg atcggcgatg ttctcgatga acaccgccgg caccttttcc
    21661 gccgcgagct ggcggatcag gccggccacg cccttggcgg tgggctcggc gttgttcgcc
    21721 acgccgacgg gcgacaggaa gcggatgtcg taggcgcgcg cgaagtaccc gaaggccgcg
    21781 tgcgaactca ccaccttgcg gcgctcggcg ggcagcgccg cgaaggcggc acggatctcc
    21841 gcgtcgagcg cctccaactc gccgtcgtag cgtgcggccg cggcgcggta ggtggccgcg
    21901 ccgtccgggt cggcggtggc gagggcggcg gcgatgttgg cgacgtagcg gcgcacgttc
    21961 gccacgtcct gccaggcgtg cgggtcgacc gccttgccct tgccatgatc gtggccatgg
    22021 tcgtgcccgt cgtcgcgcct catctccagc gtcgagatcc ccgcgctcgc caccaccacc
    22081 gcccccttga atccgccggc gcgcgccagc cgcgtcatcc agtcgtcgaa gccgaggccg
    22141 ttgaccacca ccagcgcagc cttgccgatc tcgcgcgcgt ccgagggacg cggctggaag
    22201 gcgtgggcgt cctcgtcggg accgaccagg gtgcgcacct tgatgcgctc gccgccgacc
    22261 tgggcgacga ggtcgccgag gatgctgaag ctggtcgcca cttcgagcgc ctgggcgtgg
    22321 gcgctgccgc ccaggagcag ggcggcgcag gccgcgagga tgcgcaggcg gcgcaagcgg
    22381 taggggaatc tcatcgtcgt cgggtccggg ttgaagaaag gaaagaaggt gtactcgagc
    22441 cgcagggccc caccgcggat ttccgccgcg atcgctttcg taggagcgac gcaagtcgcg
    22501 actccggtcg cagggcggtc gctcgtgtcg gcgactgacc aagcgctgcg atcgcgactt
    22561 gcgtcgcgcc cacaagagcc atcacgtctc cgcgtcgctc ctgccggagc agtcgagctg
    22621 cccgcaggcg acatcgggcc tcacgactcg aggtggtgcc gtaccggcag gcgtccggcg
    22681 agcaggccac cgggggccgc gagcagcgac acgaaataga ccacgccgca caccagcacg
    22741 atcgccggcc cggcaggcac gtcggcatgg aaggacagca gcagcccgct cgcgccgctg
    22801 ccgaaggcga acagcaccgc gagcagcagc agcatcggca ggccgttcgt ccacaggcgc
    22861 gccgcggccg cgggcaggat catgatgcct accgccatca gcgtgccgag cgcgtggaag
    22921 ccgctcacca ggttcaccac caccagcatc aggaagccgt agtgcgccac cggcgaccac
    22981 cggctcaccc cgcgcaggaa ggcggggtcg aagcactcca tgacgagcgg gcggaacagc
    23041 agcgccaggc tcgccagggt cacgctggcg atgctcgcga tcagcagcag cgaggcgtcg
    23101 tcgagcccga gcacggagcc gaagaggacg tgcagcaggt cgaggttctg cccgcgcagc
    23161 gacacgatca tgacccccgc ggcgagcgag agcaggtaga aggcggccag gctggcgtcc
    23221 tccttgagca ccgaggcgcg cgtcaccagc cccgcggcga acaccaccac caggccggcg
    23281 acgatgccgc ccaccgtcat cgcgcccagt gacaggccga aggcgaggta gccgagcgcg
    23341 gcgccgggca ggatggcgtg gctcatcgcg tcgcccatca ggctcatgcg gcgcagcagc
    23401 aggaacacgc ccaccggcgt ggcgccgagc gcgagcgcca ggcagccggc gagcgcgcgg
    23461 cgcatgaagg cgaactcggt gaagggggag atgaggaggt cgaacactgc gaaggcctgg
    23521 tttgctggcg cgggttgcgg attcagtggc ggggaaggcc gcggcgcgga cgcggcgcgg
    23581 tgtgtgccgc ggggtgcgga tgggcgtggc cgtggccttc gtcgtgcgca tgtccatggt
    23641 catgcgcgtg cggatgtgcc gccgcatcgg gcgccaccgc gaccgtcaac tcctcgatgt
    23701 ggcattcgtg cgcgtgctcg tcgaaggctt ccgacaggcg gcgcgcgcgc gccaggcgct
    23761 cgggcgtcag cacctcggcg gtggggccga aggccaccgg ctcgcgcgac agcagcagtg
    23821 cggtcgggaa gtggcggcgc acctgctcga ggtcgtgcac cacggtgagg atggtgcggc
    23881 cctcgtcgtg ccagcccagg atcagctcca cgagatcgtc caccgtgcgg ctgtcgatgg
    23941 ccgcgaaggg ctcgtcgagc aggatcagcg gcgcgtcctg caacatcagg cgggcgaaac
    24001 gcgcgcgctg cagttggccg cccgagagcg atccgatggg gcgcgactcg aagcccgcca
    24061 tgcccaccgc ctcgagcgcg gcgcgcacgc gtcggcgctg ctcgcgcgag acgccgccga
    24121 aggcgccgat ctcgtgccac aggcccatcg cgaccacgtc gaacaccgac accgggaagc
    24181 tgtggtcgat ctcgccgcgc tgtggcatgt aggccagtcc ccccgcgggc agctcgggca
    24241 gcaggatgcg cccgccgatc ggccgcagct cgccggcgag gcccttgagc agggtggatt
    24301 tgcctgcgcc gttggggccc accacggcga cgagcgcgcc cgcgggaatc tcggccgaca
    24361 ggtgatgcac cgccgggtgg cggtcgtagc ccagggtgag gttgtcgagg cggatcatcg
    24421 gcagcgcgag gcggttcatt gcaaggccca cagcacggtc gcccatagca gcgccagcgc
    24481 gccggcgacc agggcgaggc gcgcggccag gcccgcgccc agcaggctac gtgccgggaa
    24541 ggggcgcgtg tcgtcgtcgg cttggggatc ggtgccgtgc aggacggggg gcgcgcgctt
    24601 catcgttcct tcctctccac gccctcgcgg ccgcaatcgg cgcacgcgcc atgcagcacc
    24661 agctcggcgc gtgcgaccgc gaagccggcg ggcagggtcg gcgcggcggc ggacacgtcc
    24721 tccaggcaca cgatctggcc acagcgctcg cagtggaaat gggcgtggcg gtggtcgccg
    24781 tcgcggcgga tctcgaagcg ccaggcgcgt tcgccccccg cgattcgctg cgcaataccc
    24841 tgttccacca gccagtcgag cgcgcggtag agggtgacac ggtcgtgggc gatgcccgcc
    24901 ccgtcgagcg cggcgccgag ctcgtcgtgc gagagcggat gggcgctgcg ctgcaaggcc
    24961 tggatcaccg ccacccgcgt gcgcgtgacg cggccgccgc gtgcggcgat gagctcgcgg
    25021 gcagcgtcgg tggcggcagg gtgttcgtgc aggggcatat cgaagcctgt tgcggcgggg
    25081 cgcggggaaa cggctcttgt ggcagggcgg tcgcttttga tgcaacattg tagcattaga
    25141 cttgcgtgcc ccgcgctgca tggttttcag cactcgtcac gcgccccgaa tcgcttgtct
    25201 ccacttccca cgaccatgcc agcctgcttg cgtccgatcc tcctctccag cgtcatcgtc
    25261 ctggccctcg cgctccctgt cgccgcgccg gcacagcatg cgcatggcgc acaccagcac
    25321 ggcgtcgccg aactgaccgt tgtactggaa ggccgcatgt tgcagctgga gctgatctcg
    25381 ccgctcgaca acctcgtcgg cttcgagcat gcgccggcga acgagacgca gcgcggtgcg
    25441 ctcgccgatg cggagcgcct cctgctcgac agcgaggcgg tgttcgctct ccccccggaa
    25501 gctggctgcg tgatccgtga ggtcggcatc gaatcgccgt ggcgggacag cgggcgcgaa
    25561 cacagtcacg gccaaacacg cgaccacaac cggccgcccc gcggcgatca cgaggacatg
    25621 gtggcaagct acggtttcga gtgcgcgcat cccgaggcgc tgcgtcggct ggagttgcgc
    25681 gtcttcaagc acttcccgcg cctgcagcgc gtgcgcgccg agttcgccac cccgcgcggc
    25741 caggggggcg gtgtgctcac ctcgggcgcc gccgcgctcg cgctgt
//
LOCUS       DAOQNN010000007        25598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_595_length_25598_cov_10.865208, whole genome shotgun sequence.
ACCESSION   DAOQNN010000007 DAOQNN010000000
VERSION     DAOQNN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 25598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25598
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_595_length_25598_cov_10.865208"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(459..1592)
                     /locus_tag="PKV97_01170"
     CDS             complement(459..1592)
                     /locus_tag="PKV97_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNB04513.1"
                     /translation="MILTQEQELIRDSMRAFAQERLAPFAAEWDRNHTFPREALNELA
                     ELGALGMVVPEEWGGAGMDYMSLVLTLEEIAAGDGATSTIVSVQNSLPCGILNRFGSD
                     AQKEAWLKPLARGEKLGCFCLTEPHVGSDASAIRTSAVRDGNEWVLNGVKQFITTGKH
                     ADVAIVFAVTDKAAGKKGITCFLVPANAPGYQVGRIEEKMGQKASDTTQILFEDCRIP
                     ADSVVGKEGEGYRIALSNLEAGRIGIAAQCLGMARAALEAAVKYAQERESFGKPIFEH
                     QAVNFRLADMATQLEAARQLVWHAASLKDAGRPCLKEASMAKLFASEMAERVCSDAIQ
                     VHGGYGYVTDFPVERIYRDVRVCQIYEGASDIQKLVIGRALAG"
     gene            complement(1726..2934)
                     /locus_tag="PKV97_01175"
     CDS             complement(1726..2934)
                     /locus_tag="PKV97_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNB04514.1"
                     /translation="MSDPVVIVSVARTPLGGFQGDLSSLTTPQLGAIAIKAAVERAGV
                     APAQVQEVIMGCVLPAGLGQAPARQAALGAGLPLSAGCTTINKVCGSGMKATMLAHDL
                     ILAGSNEVMVAGGMESMSNAPYLLPKARAGYRLGHGQMFDHMFLDGLEDSYSKENKGR
                     LMGTFAEDCAGHFNFTRQAQDEFAIASTTRAQAAINNGDFSWEVVPVTVASRKGDVVV
                     DKDEQPLKAQIDKIPSLKPAFKKDGTVTPANSSSISDGAAALVLMRKSTAEKLGVKPV
                     ATIIGHATHAQEPALFTTAPVGAMQKVLAKAGWSTEQVDLWEINEAFAVVTMAAMHEM
                     KLPHDKVNVNGGACALGHPIGASGARILVSLIGALRKRGLKRGVASLCIGGGEATAMA
                     IEVDSSVACG"
     gene            complement(2981..4123)
                     /gene="can"
                     /locus_tag="PKV97_01180"
     CDS             complement(2981..4123)
                     /gene="can"
                     /locus_tag="PKV97_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNB04515.1"
                     /translation="MPTRLCALGANDTVELEHVRGFFRQYAAWLGVDLSFQGFADEIA
                     NLPGAYGAADGRLFYAEVDGKPAGCVGIRRFSEGVCEMKRLYVDPAFRGGGVGRKLAL
                     AAIKAARLFGYRRILLDTIPSMRIAVKLYRELGFKEVPACYPSPIEGAIFLTLDLENW
                     SEDEVSNENLFHLFDYNQAWSRQMQQLDPGFFGKLAQLQAPEYLWIGCSDSRVPANQI
                     VGLLPGEVFVHRNIANVIVHTDLNALAVIQYAVDVLQVKHIMVVGHYGCGGVKAALER
                     ARVGLVDLWLRHVQDVHVRHLKAVDGLAPELRHDRLCELNVIEQVANVAQTVVVQDAW
                     RRGQRLTVHGWIYGLQDGLIRDLGMNLSRSDDLMPRYVAALEALGN"
     gene            complement(4183..4650)
                     /locus_tag="PKV97_01185"
     CDS             complement(4183..4650)
                     /locus_tag="PKV97_01185"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNB04516.1"
                     /translation="MRPELPQFQPRDPGYAERVRASFALQQAMRLIGAEIPVVEPGYT
                     EIHLPFKAEISQQHGFVHGGVVGMIADSAAGYAANTLTPADTSVLTVEYKLNLVAPAD
                     GERLVARGEVVKPGRTLIITKAEVFAIRDGKWTLCALMQQTIMAMHGKKERAA"
     gene            4802..5836
                     /locus_tag="PKV97_01190"
     CDS             4802..5836
                     /locus_tag="PKV97_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB04517.1"
                     /translation="MSPPDPTVLEYPFDSLPSPGHSIEIAPGLRWVRMRLPFALDHIN
                     LWLLDEGEALAVVDCGFARDETREAWDAVLAADGRPLRRVVVTHHHPDHIGLATWLAA
                     RDDAAIHMTQGEFFAAQAVWHQLPGYCATDMVEQFRRHGLDEMRRDALLTRGNAYRIG
                     APQVPGRYERMFAGDELRIGAHTWRVIVGFGHAPEHASLYCAELGVLIAGDMLLPRIS
                     TNVSVYAATPHGDALGWFLHSLREFCTLPDDTLVLPSHGKPFKGIRARVEQLVEHHRE
                     RCEVLVAACAMPQSAAALLPTLFPRELDTHQVMFAMGEAIAHLNHLEKRGELSRRVDA
                     DGLIRFTHCP"
     gene            5886..7709
                     /gene="phaC"
                     /locus_tag="PKV97_01195"
     CDS             5886..7709
                     /gene="phaC"
                     /locus_tag="PKV97_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNB04518.1"
                     /translation="MAAPSNETPACDLPDPQEVAKTYAEVARRASHLISDHVHRQLKK
                     GVSTPADELGIAQAFMDMMAKLLANPYKLAQAQMNLVWDYFSLWQHSMMRVAGLSASP
                     VAAPEKSDKRFKDEEWEQHFLFDFIKQSYLIAARHIHDTVSGVDGLDEQTQKKVTFYT
                     RQYIDALSPSNFAMTNPEVFRETVKSHGQNLLKGLNNLLRDVEDGGGNLRVKMTDTSA
                     FELGKNVATTPGKVVFQTEMMQLLQYTPSTEKVLKKPLLIVPPWINKFYILDLREKNS
                     YIKWAVDQGHTVFVISWVNPDERLAQKSFDAYVKEGVIAALDAIERQTGEKEVNAAGY
                     CLGGTLLATTLAYLAAKRQKRILSATFFTTMTDFTDPGELGVFIDEGQVSSLEKKMFE
                     RGYLEGSEMAGTFNMLRANDLIWSFVVNNYLLGKDPFPFDLLYWNSDSTRMPAAMHSF
                     YLRTMYMENRLIEPGGIEIDGTPIDLGKIKVPCYFISTIEDHIAPWKSTYMGARRFGG
                     PTRFVLGGSGHIAGIVNPPAANKYGYWLNPSAKLAETADAWLEGAQQHPGSWWTDWQA
                     WVTAHDSEQTEARDPVNGKLEVLEDAPGSFVKIRIDAKAAA"
     gene            7807..8283
                     /locus_tag="PKV97_01200"
     CDS             7807..8283
                     /locus_tag="PKV97_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNB04519.1"
                     /translation="MTEPSSQAPLPPTLVLDTNTVLALWMFRDPALEALRAWIDAGSC
                     RLVSRADALEELRRVLAYAQFGLDPGRQDGLRARYIDRIAVLADDADAGTLPSCRDPD
                     DQKFLEIAGAAGMATLITRDKALLRLARHRLVRERFAILTPERVQHTLAQPRATPA"
     gene            complement(8293..9132)
                     /locus_tag="PKV97_01205"
     CDS             complement(8293..9132)
                     /locus_tag="PKV97_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNB04520.1"
                     /translation="MHSKASQDGCPTWIDAAGRLHSPAGADARIVSLVPSLTELVFDL
                     GLGERLVGRTGFCVHPREALRAVPKVGGTKDVNLARIRELAPTHVLVNMDENRRETVD
                     ALAAFVPHIVVTHPNTPQDNLVLFALLGGVFGAEGAAARLAHELRAVLDEAAALRAAL
                     ADERVLYLIWREPWMTVARDTYIAATLAEVGWSTLPAVEGGETGAARYPAFDWNAPWL
                     AGVERVLLSTEPYRFRAAHLAEVARLAARPALLVDGEMVSWYGSRAARGLRWLMALRR
                     GLA"
     gene            9404..10372
                     /locus_tag="PKV97_01210"
     CDS             9404..10372
                     /locus_tag="PKV97_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNB04521.1"
                     /translation="MKRLTALAAAFAIAGSLGAAPAHAQQKFITIGTGGVTGVYYAAG
                     GAICRLINKDRAQHGVRCSVESTGGSVYNVNTIKAGELDFGVSQSDIQYNALKGEVQF
                     KDGGAFADLRSVFSLHGEPLTVLARKEANIGKMEDFKGKRFNIGNPGSGQRATLEVLL
                     PAMGMSFADFSLASELKPDEHGAALCDNKIDGFAFVVGHPAANIQDPTTTCGAKLVPI
                     TGAGVDKILAQYPYFAPVTIPGGMYANNPDATKTFGVVASIVTSAKVPVESVYIMVKA
                     VFENFEDFKKLHPAFANLQPQDMIKNGMSAPLHEGAVKYYKEKGWM"
     gene            10496..13102
                     /locus_tag="PKV97_01215"
     CDS             10496..13102
                     /locus_tag="PKV97_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HNB04522.1"
                     /translation="MASPEKQEHGFELTDEQLRQIVAEADTGGRKPTGWPARLIVLVA
                     LAWSLMQLWIASPLPFSLGMFVFNDTEARSIHLALAVFLAFLAYPALKRSPRDRIPLG
                     DWVLAAVGAFCMGYLFLFYRELAARPGLPTTTDLAVAVCGLILLLEAARRALGLPMVV
                     LSVVFIGYVFLGPYMPEMIAHRGASLAKGMSHLWLTTEGVFGVALGVSTSFIFLFVLF
                     GSLLETAGAGNYFIKSAISLLGHLRGGPAKAAVVASASTGLISGSSIANVVTTGTFTI
                     PLMKRVGYAPKKAAAVEAAASVDGQIMPPVMGAAAFLMVEYVGIPYTEVIKHAFLPAL
                     ISYIALFYVVHLEALKADIRGLPRRVTKSAGHRLLSFGLTVSGLIVLAGAVYYGMGWI
                     KALAGDASAIIVAVLMFGCYIGLLWYATRHPELHMDDPSSPLTELPETGPTLKTGLYY
                     LLPVVALIWNLMVEELSPALSAFWATLFMIFILVTQRPLKAIFRNTGNVGEATRQGFA
                     DLHTGLVTGARNMIGIGIATATAGIIVGTVTLTGVGLVLTEFVEAVSGGNLMVMLVMV
                     AVICLILGMGLPTTANYIVVSTLMAPVVVEVGAQSGLLVPLIAVHMFVFYFGLMADVT
                     PPVGLASYAAAGIARCDPIAAGVQAFLYSMRTAILPFMFIFNTQLLMIGVDSVWLFAL
                     TVSSAIVANLMFAAATQGWFITRNRVHESILLLLVTFTLFRPGFWMDMLYPPYQELPA
                     SELTRLVEEAPRNDNLRIWVEGISLEGEEVSKGMLLPLGDTAPSARERLGRVGLTLMS
                     LGDEVQVAAVRFGSQSEKLGLEQGYKIARIEVPSVDRPSREWMFVPALALLGFVWFAQ
                     TRRARRQQPTRA"
     gene            complement(13179..13607)
                     /locus_tag="PKV97_01220"
     CDS             complement(13179..13607)
                     /locus_tag="PKV97_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNB04523.1"
                     /translation="MSDRGASAVPQAVEAAERRRIQRFLAQRRGQPWFWVMIGDERIE
                     LRDISVDGFAIAAETPPAGGEPFDFVLQREGVPDHIRGRAQAVNFIAASGQAGCRFRA
                     LEGDGAERLRDWLIALVIMNASVRISEKDAAAIVEGPSLI"
     gene            complement(13610..14236)
                     /locus_tag="PKV97_01225"
     CDS             complement(13610..14236)
                     /locus_tag="PKV97_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HNB04524.1"
                     /translation="MNRRDALKLAGLALALPAASVVQANEPFQIIEPAQKADDPSKIE
                     VLEFFHYGCPHCRDFHPLITAWKKKLPADVAFRAVPAVWNNAQLSGLARLFYAAQITG
                     DLTALEPAIFAAVQDDKRQLFNEQQVSEWVAGKVGDAAKFVETYKSFGVGSMVQRADQ
                     LARALKIQGVPSMVVGGRYLTSASLTGSHENTLKVADELIARVRKERG"
     gene            complement(14310..14978)
                     /locus_tag="PKV97_01230"
     CDS             complement(14310..14978)
                     /locus_tag="PKV97_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB04525.1"
                     /translation="MSRDRGPVRKPARAPAKSAGGTLIGIFIGLVFGALIAAGAAWYF
                     TRSNPFQAAPVAPRVQAPAEQGPAALPGKPGDRPVAKQDFEFYRILPQGDNVPSSAAQ
                     VVPVPAPQVQAPPPPVPAQQAPAPVFEASPQPAAVTPPAPQRNERIYLQVGSFENPSE
                     ADNLKARLALSGIQASAQRTQLPDGRIVHRVRVGPFAKPEDMNPMRARLSDAGFNASV
                     SRNQ"
     gene            complement(14995..>16632)
                     /gene="argS"
                     /locus_tag="PKV97_01235"
     CDS             complement(14995..>16632)
                     /gene="argS"
                     /locus_tag="PKV97_01235"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNB04526.1"
                     /translation="ATNLALQLAKPLKRNPRELAGLLLAELPPSKLVAKAEVAGAGFI
                     NFTLASDAKTAVVREVLARGADFGRGAGKGIKVQVEFVSANPTGPLHVGHGRGAAYGA
                     SLSDVLAFAGYEVSREYYVNDAGRQMDILALSTWLRYLAFFGIEIPFPPNAYQGDYVV
                     DMGREMRDAHQDRFAKVTAMQILAGTPGLPPSDRKDDEAKQQREEHLDALIANAKKLL
                     GEDYPWVHGFALNEQLGDGREDLQEFGVRFDKWFSEQSLFDTGLVERAVIQLEKAGHI
                     YLQDGAKWFRSTAFGDEKDRVVQRENGLFTYFASDIAYHLNKYERGFDRMIDIWGADH
                     HGYIPRVKGAIAALGLDPARLDVALVQFAVLYRDGQKTSMSTRSGEFVTLRELRREVG
                     NDACRFFYVLRKSDQHLDFDLDLAKSQSNENPVYYVQYAHARVCSVLDQWGGEAAELA
                     GADLGLLQNERELALCARLASFAELVQDAAAGYAPHQIAFYLKDLAADFHSWYNAERM
                     LVEDEALKLARLALAAAVRQVLVTGLKMLGVSAPESM"
     assembly_gap    16633..16732
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16733..16913)
                     /gene="argS"
                     /locus_tag="PKV97_01240"
     CDS             complement(<16733..16913)
                     /gene="argS"
                     /locus_tag="PKV97_01240"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939794.1"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNB04527.1"
                     /translation="MSADPKVLLTDLLQTALKSVAPDLAATPILLERPKQASHGDFAT
                     NLALQLAKPLKRNPRE"
     gene            17074..18603
                     /gene="ubiB"
                     /locus_tag="PKV97_01245"
     CDS             17074..18603
                     /gene="ubiB"
                     /locus_tag="PKV97_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HNB04528.1"
                     /translation="MRLFRLAKIVSVSLRFGLDRMVLDADSSGRLAHAWHKVFFWRTF
                     SESRGARLRQALESLGPIFVKFGQMLSTRRDLLPPDLADELALLQDRVPPFPTEQALE
                     VLEGFYGRPIDEVFDDFERTPVASASVAQVHFARLPDGTDVAVKVLRPGIERVIEHDL
                     ALLEVAAVLLEKIWPEGRRLKPREVVAEFSKYLHDELDLMREAANCSQLRRNFTDSSL
                     LIVPEVYWDWCGSKVMVMERMRGVPISQTPALLAQGTDLKALSRAGVEIFFTQVFRDG
                     FFHADMHPGNIFVHSDGRYIALDFGIMGTLNEVDKNYLATNFLAFFKRDYRRVALAHI
                     EAGWVPAKTRVDEFEAAIRTVCEPIFDKPLKDISFGKTLLRLFQTARRFEMEVQPQLV
                     LLQKTLLNIEGLGRQLDPELDLWKTAKPFLERWMDERMGVRALVRGVKEEAPAWAGTL
                     PQLPRLVHQALTESARHQSAQQQRLEELAAGQRTQGRLLLFIGAVAMGLLGLELYRLF
                     G"
     gene            18770..20230
                     /locus_tag="PKV97_01250"
     CDS             18770..20230
                     /locus_tag="PKV97_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004378848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNB04529.1"
                     /translation="MLLWLVVAYLVVSIGIGLYAATRVHNARDYITAGRNLPMIVVLA
                     MVFATWFGAETVLGISATFLEEGFHGLISDPLGASACLVLFGLVFARPLYRMNLLTLG
                     DFFRVRYNRTTELVLSICIVLSYLGWVAAQVTALGLVFNVLSGDAVTMNQGMLIGAGV
                     VLVYTLFGGMWSVAMTTFVQMIVIVIGLVWVSWIAGDMAGGFDNVIAKAAAEGKFEFL
                     PALDAVEIVAWIAAFATMALGSIPQQDVFQRVNSSKNERVAIWGTTLGGVSYFFFAAV
                     PLFLAYTANIIDPGMVERLMEQDSQLILPTLILQYMPFYAQVIFFGALLSVIMSTASG
                     TLLAPSVTFSENVLRGFFPGMGDRAFLLSTRVTVVLFTAFVTWYATSTESSIHEMVEN
                     AYRVTLAGAFVPLAAGLFWKRANNLGAALAIILGLSTWILFELFVPEGDIEPQLYGVA
                     ASALGMLAGGLIGRQPHHRPHIGGHHAAAGTHHTHR"
     gene            20357..21373
                     /locus_tag="PKV97_01255"
     CDS             20357..21373
                     /locus_tag="PKV97_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNB04530.1"
                     /translation="MSVSSPIELVCPAGSLPALKTAIDHGADCVYLGFKDATNARNFS
                     GLNFDPAQVREGVAYAHARKRKVLLALNTYPQTDNWSSWTQAIDRAADYGVDAVILAD
                     PGLMAYAAKTHPQLRLHLSVQGSATSYEAINWYRERFGIQRAVLPRVLSMAQVESVVA
                     NTPVEIEVFGFGGLCVMVEGRCALSAYATGESPNCNGACSPGKHVRWEDTPRGMETRL
                     NGILIDRFAEGERAGYPTLCKGRFEVNDETYYALEEPTSLNTLELLPELARIGIRAIK
                     IEGRQRSPAYVAQVTKVWRAALDKLNSGGAAGFSVLPAWMAELNKVSEGQSHTLGAYY
                     RPWK"
     gene            21491..22387
                     /locus_tag="PKV97_01260"
     CDS             21491..22387
                     /locus_tag="PKV97_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNB04531.1"
                     /translation="MKLALGPLLYYWPHQSVLDFYADLAEAPLDSVYLGETVCSRRHE
                     LRLDDWLEVGKVLAAAGKEVVISSQALIESESDLKTLRRIVRQHDFRVEANDMGAVRL
                     LVEAGVDNWIAGPTLNIFNPTTLQLMIDSGATRWAVAPEMSGAALAEVRAALARPIET
                     EVFAYGRLPLAHSARCFTARHFNLQKDTCEFRCLSIMDGITLRTREGAPFLTLNGVQT
                     QSARVHNLLGDLPAVRASAEVLRISPQGAHTRQIVELFRAALDEQRGPADALAASREW
                     MVEEPCNGFWHGRAGVEQYVAA"
     gene            22424..22960
                     /locus_tag="PKV97_01265"
     CDS             22424..22960
                     /locus_tag="PKV97_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNB04532.1"
                     /translation="MPALPALPRPPLPSHLLPGSLRQRIGERLATLRIPDFTVPAPVA
                     KLVSRLPQQPPTHVLTLALNLALDRILPRDTLEPLSGRHLQMRVLDAGLTLDFTLGER
                     GFRRATPAAGARPDLILSATTRDFMALALREEDPDTLFFSRRLRMEGDTDLGLLVKNT
                     LDAVDWDALKAKLLGRHG"
     assembly_gap    22968..23067
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23169..24128
                     /gene="thiL"
                     /locus_tag="PKV97_01270"
     CDS             23169..24128
                     /gene="thiL"
                     /locus_tag="PKV97_01270"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNB04533.1"
                     /translation="MPSEFDLIRRHFTRPARHTELAVGDDAALLAPRPGMRLAVSTDM
                     LVAGTHFFADTDPEGLGWKTLAVNVSDLAAMGAEPRWAFLALALPQADEAWIAAFARG
                     FFACADAFAIDLAGGDTTRGPLTLSVTIVGEVPAGAAITRGGARAGDDLWISGQPGRA
                     ALGLAALRGETGLDAIHHAEFVDALQRPQPRVALGLALRSLASAMLDVSDGLLGDLRH
                     LLECSGTGAIVEVAALPLAALAASGADPALCRRCLLAGGDDYELLFAAAPRHRAEIDA
                     LSARFDLPLHRIGEFTPARERLLLREADGGLAAPAACGYDHFA"
     gene            24160..24642
                     /locus_tag="PKV97_01275"
     CDS             24160..24642
                     /locus_tag="PKV97_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNB04534.1"
                     /translation="MRPTARLLMSHPAHFISLGFGAGLSPWAPGTVGTLLGWAIYPLI
                     RTPVSEFVFLALLASFFLAGVLAAERTGQALGVPDHGGIVWDEMVAIWLVLLFTPAGL
                     LWQAIAVALFRFFDIVKPPPVRWADRSFKGGFGVMFDDLVAAGYTLLALAVLVHLLGS
                     "
     gene            24647..25135
                     /gene="pncC"
                     /locus_tag="PKV97_01280"
     CDS             24647..25135
                     /gene="pncC"
                     /locus_tag="PKV97_01280"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HNB04535.1"
                     /translation="MTDHELDALARRVGEALANRRLWLATAESCTGGWIAEAVTAIAG
                     SSGWFDRGFVTYSNDAKADMLGVRATTLETHGAVSEATVGEMAAGALDHSRADLAVAV
                     SGVAGPGGGSPAKPVGTVCLAWARRDGPTGVVTVHLDGDRAAVRRQTVILALQGVLEH
                     CV"
     gene            25332..>25598
                     /locus_tag="PKV97_01285"
     CDS             25332..>25598
                     /locus_tag="PKV97_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HNB04536.1"
                     /translation="MDENKAKALSAALSQIEKQFGKGSIMRMGDGSIERDIQTVSTGS
                     LGLDIALGLGGLPRGRVVEIYGPESSGKTTLTLQVIAEMQKLGGT"
BASE COUNT         3919 a         9034 c         8355 g         4090 t
ORIGIN      
        1 cctcgccacg cgcggccgcg ctcagcgtga ccaccgcgct cggctgcgcg acctggcttg
       61 ccgaggcgtc acgtgctggg ggttcctgcg cacgcgccgc ctgttcgggc gcggcggcct
      121 gcgcctgacg cggctcgctt ggccgcatca gctcgcgggt gacggaattc attgcagggg
      181 acacggcttc catgacgctc gcctcgctga tcgttcttca agcttcttag cgcgcccggg
      241 cctgcaagca caaagccatt cattcgcagc ccgctccgac gctcctgcag cggatgttct
      301 cacgaacgcg gccgctgcgg ctgtggcacg ggtcacgacc gcccggcaga ttgcccacga
      361 ggggcgacgg cggtgcagga aacagcttcc cgagccgcac aggacgcaag gccgcccccc
      421 gcccagggaa gccggcctgc agccgcgcca gcgcaggctc agcccgccag cgcgcgcccg
      481 atcaccagct tctggatgtc gctggcgcct tcgtagatct ggcacacgcg cacgtcgcgg
      541 tagatgcgct cgaccgggaa gtcggtgacg tagccatagc cgccgtgcac ctggatggcg
      601 tccgagcaca cgcgctcggc catctcggag gcgaacagct tggccatcga ggcctccttg
      661 aggcaggggc ggcccgcgtc cttcaggctg gcggcgtgcc acacgagctg gcgcgcagcc
      721 tccagttgcg tggccatgtc ggcgaggcgg aagttgaccg cctggtgctc gaagatcggc
      781 ttgccgaagc tctcgcgctc ctgggcgtac ttgaccgcag cctccagcgc cgcacgcgcc
      841 atgccgagac actgcgcggc gatgccgata cggccggcct cgaggttcga cagcgcgatc
      901 ctgtagccct cgccttcctt gccgaccacc gaatccgcgg ggatgcggca atcctcgaag
      961 aggatctggg tggtgtcgga ggccttctgc cccatcttct cctcgatgcg gcccacctgg
     1021 tagccgggcg cattggccgg caccaggaag caggtgatgc ccttcttgcc ggcggccttg
     1081 tcggtcaccg cgaacacgat ggcaacatcg gcgtgcttgc cggtggtgat gaactgcttg
     1141 acgccgttca acacccactc gttgccgtcg cggaccgcgc tggtgcggat cgccgaagcg
     1201 tccgagccca catgcggctc ggtcagacag aagcagccga gcttctcgcc gcgggcgagc
     1261 ggcttcaacc acgcctcctt ctgcgcgtcg ctgccgaagc ggttgaggat gccgcagggc
     1321 agcgagttct gcacgctgac gatggtcgag gtggcgccgt cgccggcggc gatctcttcc
     1381 agcgtgagca ccaggctcat gtagtccatg ccggcgccgc cccattcctc gggcaccacc
     1441 atgccgagcg cgccgagttc ggcgagctcg ttcagcgcct cgcgcgggaa ggtgtggtta
     1501 cggtcccatt cggcagcgaa gggggccagg cgctcctgcg cgaaggcacg catggagtcg
     1561 cggatgagtt cctgttcctg ggtgagaatc attgcactgc tgtccttgta gaaatccttg
     1621 ctggtgtggc gttgcgcgtt gcgggggcgc cccggcgggt cgcccctaac gaaacgaggg
     1681 tcctggcagg gtcgtctcgc gagcggccgc agcgcttgat ccgattcagc cgcaggccac
     1741 cgacgaatcg acctcgatcg ccatcgcggt ggcttcgccg ccgccgatgc acaggctggc
     1801 cacgccgcgc ttgaggccgc gcttgcgcag cgcgccgatc agcgagacca ggatgcgcgc
     1861 gcccgaagcg ccgatcgggt ggccgagcgc gcaggcgccg ccgttcacgt tgaccttgtc
     1921 gtgcggcagc ttcatctcgt gcatcgccgc catggtgacg accgcgaagg cctcgttgat
     1981 ctcccacagg tcgacctgct cggtgctcca gccggccttc gccagcactt tctgcatcgc
     2041 gcccaccggc gcggtggtga acagcgccgg ttcctgcgca tgcgtggcgt ggccgatgat
     2101 ggtcgcgacc ggcttcacgc cgagcttctc ggcggtggac ttgcgcatca gcaccagcgc
     2161 ggcggcgccg tccgagatgg agctcgagtt ggccggggta acggtaccgt ccttcttgaa
     2221 cgccggcttg agcgagggga tcttgtcgat ctgcgccttc agcggctgct cgtccttgtc
     2281 gaccaccacg tcgcccttgc ggctggccac ggtgaccggc accacctccc agctgaagtc
     2341 gccgttgttg atcgcggcct gcgcgcgcgt ggtcgaggcg atcgcgaact cgtcctgggc
     2401 ctggcgggtg aagttgaagt ggccggcgca atcctcggcg aaggtgccca tgaggcggcc
     2461 cttgttctcc ttggagtagc tgtcttccag gccgtcgagg aacatgtggt cgaacatctg
     2521 gccgtggccg aggcgatagc cggcacgcgc cttgggcagc aggtaggggg cgttggacat
     2581 cgactccatg ccaccggcga ccatgacctc gttgctgccg gcgaggatga ggtcgtgcgc
     2641 cagcatggtg gccttcatgc ccgagccgca caccttgttg atcgtggtgc agccggccga
     2701 cagcggcagg ccggcgccga gcgcggcctg gcgggcaggc gcctggccga gaccggcggg
     2761 cagcacgcag cccatgatca cttcctgcac ctgcgcgggc gcgacgccgg cacgctcgac
     2821 cgcggccttg atcgcgatgg cgccgagctg cggcgtggtg aggctggaca ggtcgccctg
     2881 gaaaccaccc agcggggtgc gggcgacgga aacgatgacg acgggatcgg acatggaact
     2941 ctcctgttat gggtgttgat cgattcgatt tgcgcggcgc tcagttgccg agcgcctcca
     3001 gcgccgcgac gtagcgcggc atgagatcgt cggaacggct gaggttcatc ccgaggtcgc
     3061 ggatcagacc gtcctgcagg ccgtagatcc agccatgcac ggtgaggcgc tggccgcgcc
     3121 gccaggcgtc ctgcacgacc acggtctgcg ccacgttggc cacctgctcg atgacgttga
     3181 gctcgcacag gcgatcgtgg cgcagttcgg gcgccaggcc atcgacggcc ttcagatggc
     3241 gcacatggac gtcctgcacg tggcgcagcc aaagatcgac gaggccgacg cgcgcacgtt
     3301 cgagcgcggc cttgacgccg ccgcagccgt agtggccaac gaccatgatg tgcttcacct
     3361 gcagtacatc gaccgcgtac tggatcaccg ccagcgcgtt gaggtcggtg tgcacgatca
     3421 cgttggcgat gttgcggtgg acgaagacct cgcccggcag caggccgacg atctggttgg
     3481 ccggcacgcg cgagtcggaa cagccgatcc agaggtactc cggggcctga agctgcgcca
     3541 gcttgccgaa gaagccgggg tcgagctgct gcatctggcg cgaccacgcc tggttgtagt
     3601 cgaagaggtg gaagaggttc tcgttgctga cctcgtcctc cgaccagttc tccaggtcca
     3661 gggtcaggaa gatcgcaccc tcgatcggcg aggggtagca ggcgggcact tccttgaagc
     3721 cgagctcgcg gtagagcttg accgcgatcc gcatcgacgg gatggtgtcg agcaggatgc
     3781 ggcggtagcc gaacagacgc gccgccttga tggcggcgag cgcgagcttg cgccccacgc
     3841 cgccaccacg gaaggccgga tcgacgtaga gccgcttcat ctcgcacacg ccctcggaga
     3901 agcggcggat gcccacgcag ccggccggct tgccatcgac ctcggcgtag aacaggcggc
     3961 cgtcggcggc gccgtaggcc cccggcaggt tggcgatctc gtcggcgaac ccctggaagc
     4021 tcaggtccac gccgagccag gcggcgtact ggcggaagaa gccgcgcacg tgctcgagct
     4081 cgacggtgtc gttggcgccg agggcgcaca gacgggtggg catggcagga ctccgggaag
     4141 gttcggggac ggatctctcg ggggaggggg aagagcaagc cttcaggcgg cgcgctcctt
     4201 cttgccgtgc atggccatga tcgtctgctg catcagcgca cacaaggtcc acttgccgtc
     4261 gcggatggcg aagacctcgg ccttggtgat gatcagcgtg cgccccggct tgaccacctc
     4321 gccgcgcgcc accaggcgct cgccgtcggc gggggcgacg agattgagct tgtactcgac
     4381 ggtgagcacg ctggtgtcgg ccggggtcag ggtgttggcc gcgtagcccg cggccgagtc
     4441 ggcgatcatg ccgaccaccc cgccgtggac gaagccgtgc tgctgggaga tctccgcctt
     4501 gaagggcagg tggatctcgg tgtagccggg ctcgaccacc ggaatctcgg cgccgatcag
     4561 gcgcattgcc tgctgcaggg cgaagctggc gcgcacgcgc tcggcgtagc cggggtcgcg
     4621 gggctggaac tggggcaatt cggggcgcat cttcgtgtct ccgtcgagtg tgtaattatg
     4681 ccagccaata cgttaacgtt aacgtaatcg atcccattcc ggaacggccc gacggcgtga
     4741 gcgccgcccg cgcacgccgg tccgcacgct gaacgtgtcc gcccccagta gaggagaccc
     4801 catgagcccc cccgacccca cggtcctcga ataccccttc gacagcctgc cctcgcccgg
     4861 tcactcgatc gagatcgccc ccggcctgcg ctgggtgcgc atgcgcctgc ccttcgcgct
     4921 cgaccacatc aacctgtggc tgctcgacga aggcgaggcg ctcgcggtgg tggactgcgg
     4981 cttcgcccgc gacgagacgc gcgaggcgtg ggacgccgtg ctcgccgcgg acggccggcc
     5041 actgcgccgc gtcgtggtga cccaccacca ccccgaccac atcggcctcg ccacctggct
     5101 ggccgcgcgc gacgacgcgg cgatccacat gacccagggc gagttcttcg ccgcccaggc
     5161 agtgtggcac cagctgccgg gctattgcgc caccgacatg gtcgagcagt tccgccgcca
     5221 cggcctcgac gagatgcgcc gcgacgccct gctgacccgc ggcaacgcct accgcatcgg
     5281 tgcgccgcag gtgcccggcc gctacgagcg catgtttgcc ggcgacgagt tgcgcatcgg
     5341 cgcccatacc tggcgcgtca tcgtcggctt cggccacgca cccgagcacg ccagcctgta
     5401 ctgcgcggag ctcggcgtgt tgatcgcggg cgacatgttg ctgccgcgca tctccaccaa
     5461 cgtcagcgtt tatgctgcaa cgccgcatgg cgacgccctg ggatggtttc tccactcatt
     5521 gcgagaattc tgcaccttgc ccgacgacac gctcgtgcta ccatcacatg gcaaaccttt
     5581 caagggtatc cgggcacgcg tggagcagct cgtcgagcat catcgcgaac gttgcgaggt
     5641 gcttgtggcg gcttgtgcca tgccgcaaag cgccgcagca ttgctgccga cgctgtttcc
     5701 gcgcgagctc gatacccatc aggtcatgtt cgccatgggg gaagcgatcg ctcacctcaa
     5761 ccatctcgag aagaggggag agctctcgag acgcgtcgat gcggacggcc tgatccgttt
     5821 cacccattgt ccgtaacatt gtccgcaaca gaccgccccg ccgcaaaaac aggagcccga
     5881 acaagatggc cgcacccagc aacgagaccc ccgcctgcga cctgcccgac ccgcaggaag
     5941 tcgccaagac ctatgccgaa gtcgcccgcc gcgcctcgca cctgatcagc gaccacgtgc
     6001 accgccaact caagaagggc gtgagcaccc ccgccgacga gctcggcatc gcccaggcct
     6061 tcatggacat gatggccaag ctgctggcca acccgtacaa gctcgcccag gcgcagatga
     6121 atctggtgtg ggactacttc tcgctgtggc agcactcgat gatgcgtgtc gccggcctga
     6181 gcgcctcgcc cgtcgccgcc cccgagaaga gcgacaagcg cttcaaggac gaggagtggg
     6241 agcagcactt cctgttcgac ttcatcaagc agtcctacct gatcgccgcg cgccacatcc
     6301 acgacaccgt cagcggcgtc gacggcctcg acgagcagac gcagaagaag gtcaccttct
     6361 acacccgcca gtatatcgac gcgctgtcgc cgtcgaactt cgcgatgacc aaccccgagg
     6421 tcttccgcga gacggtcaag agccacggcc agaacctgct caagggcctc aacaacctgc
     6481 tgcgcgacgt cgaggacggc ggcggcaacc tgcgcgtcaa gatgaccgac acctcggcct
     6541 tcgagctggg caagaacgtc gccaccacgc cgggcaaggt cgttttccag accgagatga
     6601 tgcagctgct gcagtacacc ccgagcaccg aaaaggtcct caagaagccg ctgctgatcg
     6661 tgccgccctg gatcaacaag ttctacatcc tcgacctgcg cgagaagaac tcctacatca
     6721 agtgggcagt ggaccagggc cacaccgtgt tcgtgatctc ctgggtcaac cccgacgagc
     6781 gcctggcgca gaagagcttc gacgcctacg tcaaggaagg cgtcatcgcc gccctcgacg
     6841 cgatcgagcg ccagaccggc gagaaggaag tgaatgccgc gggctattgc ctgggtggga
     6901 ccctgctggc gaccacgctc gcctacctcg ccgccaagcg ccagaagcgc atcctctcgg
     6961 cgaccttctt caccaccatg accgacttca ccgatccggg cgaactcggc gtgttcatcg
     7021 acgaaggcca ggtttccagc ctcgagaaga agatgttcga gcgcggctac ctggaaggct
     7081 cggagatggc cggcaccttc aacatgctgc gcgccaacga cctcatctgg tccttcgtgg
     7141 tcaacaacta cctgctcggc aaggatccgt tcccgttcga cctgctgtac tggaactcgg
     7201 actcgacccg catgccggcg gccatgcaca gcttctacct gcgcacgatg tacatggaaa
     7261 accgcctgat cgagccgggc ggcatcgaga tcgacggcac cccgatcgac ctcggcaaga
     7321 tcaaggtgcc gtgctacttc atttccacga tcgaggatca catcgcgccg tggaagagca
     7381 cctacatggg cgcccgtcgc ttcggcggcc cgacccgctt cgtgctcggc ggctccggcc
     7441 atatcgccgg catcgtgaac ccgcccgccg ccaacaagta cggctactgg ctcaacccga
     7501 gcgcgaagct cgccgaaacc gccgacgcct ggctggaagg cgcgcagcag cacccgggct
     7561 cgtggtggac cgactggcag gcctgggtca ccgcacacga cagcgagcag accgaggcgc
     7621 gcgatccggt gaacggcaag ctcgaggtcc tcgaagacgc cccgggcagc ttcgtcaaga
     7681 tccgcatcga cgccaaggcc gccgcctgag ccttgtcgcg acgagtacgg ggaggggaag
     7741 ccctccccgt ttttcgttca ccacgctgca tcgccgcgcc ggcgatccgg ctcacccact
     7801 gcgaacgtga ccgaaccctc ctcgcaagca cccctgccgc ccaccctcgt gctcgacacc
     7861 aacacggtgc tcgcgctgtg gatgttccgc gaccccgcgc tcgaggcatt acgcgcgtgg
     7921 atcgacgcag gatcgtgccg cctggtctcg cgtgcagatg cgctggaaga gttgcgccgc
     7981 gtgctcgcct atgcgcagtt cggtctcgac ccgggccgcc aggacggcct gcgcgcacgc
     8041 tacatcgacc gcatcgccgt gctggcggac gatgccgacg ccggcacgct ccccagctgt
     8101 cgcgaccccg acgaccagaa gttcctcgaa atcgccggcg ccgccggcat ggcgacgctg
     8161 atcacccgcg acaaagccct gctgcgcctg gcgcgccacc gcctcgtacg cgagcgcttc
     8221 gccatcctca ccccggaacg agtgcagcac acgctcgcac agccgcgcgc aacgccggcc
     8281 tgagcccccg gctcacgcca gcccgcgccg cagcgccatc agccagcgca ggccgcgcgc
     8341 ggcacggcta ccgtaccacg acaccatctc gccatcgacc agcagcgccg ggcgcgcagc
     8401 caggcgcgcg acctcggcga gatgcgccgc gcggaagcgg tagggctcgg tggacagcag
     8461 cacgcgctcg actccggcga gccagggcgc attccagtcg aaggcgggat agcgcgccgc
     8521 tccggtctcg ccaccttcca ccgctggcag ggtgctccag ccgacctcgg cgagcgtggc
     8581 ggcgatgtag gtgtcgcgcg cgaccgtcat ccagggctcg cgccagatca ggtagagcac
     8641 gcgctcgtcg gcgagcgccg cgcgcaacgc cgcggcctcg tcgagcactg cgcgcagttc
     8701 gtgggcgagc cgggcggcgg ctccttcagc gccgaagacg ccgccgagca gcgcgaagag
     8761 gacgaggttg tcctgcggcg tgttgggatg ggtgacgacg atgtggggca cgaaggcggc
     8821 gagcgcgtcg acggtctcgc ggcggttctc gtccatgttg acgagaacat gcgtgggcgc
     8881 gagctcgcgg atgcgcgcga ggttgacgtc cttcgtcccg ccgaccttgg gaacggcgcg
     8941 caaggcctcg cgcgggtgta cgcagaagcc ggtgcgcccg acgaggcgct cgcccaggcc
     9001 gaggtcgaac accagttcgg tcagggaggg caccagggag acgatgcgcg cgtccgcgcc
     9061 ggcgggcgaa tgcagccgcc cggcagcgtc gatccaggtc gggcacccgt cctgtgaagc
     9121 cttggaatgc atggtcaatg ctcggatgaa acgtgcggag ccgcatctta tcgcgccaca
     9181 ccctcccgcc ctcgcaccct gcggggaatt catgcgcaag ccgcgcacct cgggaaatcc
     9241 cgagtgcagg caggaatcgt tttgtgccaa gcttggccat ccttggtggg cgctcttggc
     9301 cgctttcgcg gccacccggc tcccgccccg atccgcagca cgtgtcctcc ctgtcggcgc
     9361 cccttcggcc agccggcgtt tcaccttcac ggagaaagct cacatgaaaa gactgactgc
     9421 cctggctgcc gccttcgcaa tcgccggctc gctcggcgcc gcccccgcgc acgcccagca
     9481 gaagttcatc acgatcggta ccgggggtgt cacgggcgtc tactacgccg cgggcggcgc
     9541 gatctgccgc ctgatcaaca aggaccgcgc ccagcacggc gtgcgctgct cggtcgaaag
     9601 caccggcggt tcggtgtaca acgtgaatac catcaaggcc ggcgaactcg acttcggcgt
     9661 ctcgcagtcg gacatccagt acaacgcgct caagggcgag gtccagttca aggacggcgg
     9721 cgccttcgca gacctgcgct cggtgttctc gctgcatggc gagccgctga ccgtgctggc
     9781 gcgcaaggaa gccaacatcg gcaagatgga ggacttcaag ggcaagcgct tcaacatcgg
     9841 caacccgggt tcgggccagc gcgcgaccct cgaggtgctt ctgccggcga tgggcatgag
     9901 cttcgccgac ttctcgctcg cctccgagct caagcccgac gagcacggcg ccgcgctatg
     9961 cgacaacaag atcgacggct tcgccttcgt ggtcggccac ccggcggcca acatccagga
    10021 tccgaccacc acctgcggcg ccaagctggt gccgatcacc ggcgcgggcg tggacaagat
    10081 cctcgcccag tacccctact tcgctccggt caccatcccg ggcggcatgt atgccaacaa
    10141 cccggatgcg accaagacct tcggcgtggt ggcgagcatc gtgacctcgg ccaaggtgcc
    10201 ggtggagtcg gtctacatca tggtcaaggc ggtgttcgag aacttcgagg acttcaagaa
    10261 gctgcacccg gccttcgcca acctgcagcc gcaggacatg atcaagaacg gcatgtcggc
    10321 cccgctgcac gagggcgcgg tgaagtacta caaggaaaag ggctggatgt aagtccaccg
    10381 cttgccacat ccctggcgag caagcccgat gtcacgagaa cggcggcggt cccgaggccc
    10441 tcgccgtttt ctttccatcg tccgtcgaat gcggactttc acgacgaggt aggccatggc
    10501 cagcccggaa aaacaagaac acggtttcga gctcaccgac gaacaactcc gccagatcgt
    10561 cgccgaggcc gacacgggtg ggcgcaagcc gaccggatgg ccggcgcggc tcatcgtgct
    10621 ggtcgcgctg gcatggtccc tgatgcagct ctggatcgcc tcgccgctgc cgttctcgct
    10681 cggcatgttc gtcttcaacg acaccgaggc gcgctcgatc cacctcgcac tggccgtgtt
    10741 tctcgccttt ctcgcctacc cggcactgaa gcgctctcca cgcgaccgga tcccgctcgg
    10801 ggactgggta cttgcagcgg tcggggcgtt ctgcatgggc tacctgttcc tcttctacag
    10861 ggaactggcc gcccgccccg gcctgccgac cacgacggac ctcgcggtcg ccgtgtgcgg
    10921 cctgatcctg ctgctcgaag ccgcacgccg cgcactcggc ctgcccatgg tcgtgctgtc
    10981 ggtggtgttc atcggctacg tctttctggg cccctacatg ccggagatga tcgcccaccg
    11041 cggcgcctcg ctggcgaagg ggatgagcca cctgtggctg accaccgagg gcgtgttcgg
    11101 ggtggccctc ggggtatcga ccagcttcat cttccttttt gtgctgttcg gctccctgct
    11161 cgagaccgcg ggcgccggca actacttcat caagtcggcg atctcgctgc tcgggcatct
    11221 gcgcggcggc ccggccaagg ccgccgtggt ggcttccgcg agcacgggat tgatctccgg
    11281 ctcctcgatc gccaacgtcg tcaccaccgg caccttcacg atcccgctga tgaaacgcgt
    11341 ggggtatgcg cccaagaagg cggcggcggt cgaggccgcg gcctcggtcg atggccagat
    11401 catgccgccg gtcatgggcg ctgcggcctt cctgatggtc gagtacgtgg gcattcccta
    11461 caccgaggtc atcaagcatg ccttcctgcc ggcgctgatc tcctacatcg cgctcttcta
    11521 cgtggtgcac ctcgaggcgc tcaaggccga catccgcggg ctgccgcgcc gggtgacgaa
    11581 atccgccgga catcgcctgc tgtccttcgg cctcaccgtg tcgggcctga tcgtccttgc
    11641 cggggcggtc tattacggca tgggctggat caaggcgctg gcaggcgacg cctcggcgat
    11701 catcgtcgcg gtgctcatgt tcggctgcta catcggcctg ctgtggtacg cgacgcggca
    11761 ccctgagctg cacatggacg accccagcag ccccctgacc gagctgcccg agaccggccc
    11821 gacgctgaag acgggcctgt actacctgct gccggtggtc gcgctgatct ggaacctgat
    11881 ggtggaggag ctctcgcctg cgctgtcggc gttctgggcg accctgttca tgatcttcat
    11941 cctggtcacg cagcgcccgc tcaaggcgat cttccgcaac accggcaacg tgggtgaggc
    12001 aacccggcag ggcttcgccg acctccacac cggcctggtc accggggcgc gcaacatgat
    12061 cggcatcggc atcgcgaccg cgaccgccgg catcatcgtc ggcaccgtca cgctgaccgg
    12121 tgtcggcctg gtgctcaccg agttcgtcga ggcggtgtct ggcggcaatc tgatggtgat
    12181 gctggtcatg gtcgcggtca tctgcctgat cctcggcatg ggcctgccca ccacggcgaa
    12241 ctacatcgtg gtgtcgaccc tgatggcgcc ggtcgtggtc gaggtcggcg cgcaaagcgg
    12301 cctgctcgtc ccgctgatcg cggtgcatat gttcgtcttc tacttcggcc tgatggccga
    12361 cgtcacccca ccggtcggcc tcgcctccta cgccgccgcg gggatcgcac gctgcgaccc
    12421 gatcgctgcg ggcgtgcagg ccttcctgta ctcgatgcgg acggcgatcc tgcccttcat
    12481 gttcatcttc aacacccagt tgctgatgat cggcgtcgat tcggtgtggc tgttcgccct
    12541 gacggtatcg agtgcgatcg tggcgaacct gatgttcgcg gcggccaccc agggctggtt
    12601 catcacccgc aaccgcgtgc acgagtcgat cctgctgttg ctggtgacct tcacgctgtt
    12661 ccgcccgggc ttctggatgg acatgctgta cccaccctat caggagctgc cggcctcgga
    12721 gctgacccgc ctggtcgagg aggctccacg caacgacaac ctgcgcatct gggtggaggg
    12781 gatttcgctc gagggcgagg aagtctcgaa aggcatgctg cttccgctcg gcgacactgc
    12841 gcccagcgcc agggagcgcc tgggcagggt cgggctgacc ttgatgagcc ttggagatga
    12901 agtccaggtc gccgcggtgc gcttcgggag ccagagcgag aagctggggc tggagcaggg
    12961 gtacaagatc gcccgcatcg aggtaccgtc cgtcgaccgt ccgtcgaggg aatggatgtt
    13021 cgtgcccgcg ctcgcgctgc tgggcttcgt ctggttcgcg cagactcgcc gcgcgcgacg
    13081 gcagcagccc acgcgcgcct gaaagaccgt gggggacgga cgtcgctacc tccagagcga
    13141 cgtccgtccc ccacgactgc cgggcacgtc aggaagtttc agatcagcga cggcccctcg
    13201 acgattgccg ccgcgtcctt ctcgctgatg cgcacgctcg cgttcatgat caccaatgcg
    13261 atcagccagt cgcgcaagcg ctcggcgcca tcgccctcga gcgcgcggaa gcggcagccc
    13321 gcctgcccgc tcgcagcgat gaaattcacc gcctgcgccc gaccgcggat gtggtcggga
    13381 acgccctcgc gctgcagcac gaagtcgaaa ggctcgccgc ccgccggagg cgtttcggcg
    13441 gcgatggcga aaccatcgac cgagatgtcg cgcagctcaa tgcgctcgtc gccgatcatc
    13501 acccagaacc aaggctggcc acggcgctgg gccaggaagc gctgaatgcg gcggcgctcg
    13561 gcggcctcga ccgcctgcgg cacggcgctg gcgccacgat cgctcatctt cagccgcgct
    13621 ccttgcgtac gcgggcgatc aactcgtcgg cgaccttgag cgtattctcg tggctgccgg
    13681 tgagcgaggc cgaggtcagg tagcgtccgc cgaccaccat ggacggcacg ccctggatct
    13741 tcagcgcgcg cgcgagctgg tcggcgcgct gcaccatcga acccacgccg aaagacttgt
    13801 aggtctcgac gaacttcgcc gcatcgccca ccttgccggc gacccactcg gaaacctgct
    13861 gctcgttgaa cagctgacgc ttgtcgtcct gcaccgcggc gaagatcgcc ggttcgagcg
    13921 cggtgagatc gccggtgatc tgcgcggcgt agaacagccg ggcgagaccg ctcagctggg
    13981 cgttgttcca caccgccggc accgcgcgga aggcgacgtc ggccggcagc ttcttcttcc
    14041 acgcggtgat cagcggatga aagtcgcggc agtgcgggca gccgtagtgg aagaactcga
    14101 gcacctcgat cttcgaggga tcgtccgcct tctgtgccgg ctcgatgatc tggaagggct
    14161 cgttcgcctg cacgaccgag gcggcgggaa gggcgagggc caggccggcc agcttgaggg
    14221 catcacgacg attcatgcgg aactcctttg actgaggctg ggtgagacgc ggacggcgca
    14281 cggacgttca atccgcagcg tgtccaggct cactggttgc ggctcaccga ggcattgaaa
    14341 cccgcatcgg agaggcgggc ccgcatcggg ttcatgtcct cgggcttggc gaaggggccg
    14401 acgcgcacgc ggtgcacgat gcgcccgtcg ggcagctggg tgcgctgcgc gctcgcctgg
    14461 atgcccgaga gcgccagccg cgccttgagg ttgtccgctt cggacgggtt ctcgaacgag
    14521 cccacctgca ggtagatgcg ctcgttgcgc tggggcgcgg gcggcgtcac cgccgcgggc
    14581 tgcggcgagg cctcgaacac cggcgcgggg gcctgctgcg cgggcacggg cgggggcggc
    14641 gcctgcacct ggggcgcggg caccggcacg acctgcgccg ccgacgaagg cacgttgtcg
    14701 ccctgcggca ggatgcggta gaactcgaaa tcctgcttgg ccaccgggcg gtcgcccggc
    14761 ttgccgggca gcgctgccgg cccctgctcc gccggcgcct gcacgcgcgg cgccaccggt
    14821 gcagcctgga agggattgct gcgggtgaag taccacgccg cgccagccgc gatcagcgca
    14881 ccgaagacga ggccgatgaa gatgccgatc agcgttccgc cggcgctctt ggccggtgcg
    14941 cgcgccggct tgcggaccgg gccgcgatct cgactcacga ttgccccccc tgcatcacat
    15001 cgactcgggc gcggacacgc ccagcatctt caggccggtg accagcacct ggcgcaccgc
    15061 cgcggcgagc gcgaggcggg cgagcttgag cgcctcgtcc tcgaccagca tgcgctcggc
    15121 gttgtaccag ctatggaagt cggcagcgag atccttgagg tagaacgcga tctggtgcgg
    15181 cgcgtagccg gcggcagcgt cctgcaccag ctcggcgaag ctcgccagac gcgcgcacag
    15241 cgcgagctcg cgctcgttct gcagcaggcc gaggtcggca ccggccagct ccgcggcctc
    15301 gccgccccac tggtcgagca ccgagcacac gcgggcgtgg gcgtactgga cgtaatacac
    15361 cggattctcg ttgctctggc tcttggcgag gtcgaggtcg aagtcgaggt gctggtcgga
    15421 cttgcgcagc acgtagaaga agcggcaggc atcgttgccc acctcgcggc gcagctcgcg
    15481 cagggtgacg aactcgcccg agcgggtgga catcgaggtc ttctggccgt cgcggtagag
    15541 caccgcgaac tgcaccaagg caacgtccag tcgggccgga tcgagcccga gcgcggcgat
    15601 cgcgcccttc acgcgcggga tgtagccgtg gtggtcggcg ccccagatgt cgatcatgcg
    15661 gtcgaagccg cgctcgtact tgttgaggtg gtaggcgatg tcggaggcga agtaggtgaa
    15721 gaggccgttc tcgcgctgca cgacgcggtc cttctcgtcg ccgaaggcgg tggagcggaa
    15781 ccacttcgcg ccgtcctgga gatagatgtg accggccttc tcgagctgga tcaccgcgcg
    15841 ctcgaccagg ccggtgtcga acaggctctg ctccgagaac cacttgtcga agcgcacgcc
    15901 gaactcctgc aggtcctcgc gaccatcgcc gagctgctcg ttgagcgcga agccgtgcac
    15961 ccaagggtag tcctcgccga gcagcttctt ggcgttggcg atcagcgcgt cgaggtgttc
    16021 ctcgcgctgt tgcttggcct cgtcgtcctt gcgatcggaa ggcggcaggc cgggcgtccc
    16081 ggccaggatc tgcatggcgg tgaccttggc gaagcggtcc tggtgggcgt cgcgcatctc
    16141 gcggcccatg tcgaccacgt agtcgccctg gtaggcgttg ggcgggaagg gaatctcgat
    16201 gccgaagaag gcgaggtagc gcagccaggt cgacagcgcc aggatgtcca tctggcggcc
    16261 ggcatcgttg acgtagtact cgcggctgac ctcgtagccg gcgaaggcga gcacgtccga
    16321 gagcgaggcg ccataggcgg cgccgcgacc gtggccgacg tggagcgggc cggtggggtt
    16381 ggcggagacg aactccacct gcaccttgat gcccttgcct gccccgcggc cgaaatccgc
    16441 gcccctggcc agcacctcgc gcaccaccgc ggtcttggcg tcggaggcga gcgtgaagtt
    16501 gatgaagccg gcgccggcga cctcggcctt ggcgaccagc ttcgagggcg gcagctcggc
    16561 gagcagcagg ccggcgagct cgcgcgggtt gcgcttgagc ggcttggcga gctgcagcgc
    16621 gaggttggtg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngctcgcgc
    16741 gggttgcgct tgagcggctt ggcgagctgc agcgcgaggt tggtggcaaa gtcgccgtgg
    16801 ctcgcctgct tcgggcgttc cagcaggatg ggggtggcgg cgagatccgg cgcgacgctc
    16861 ttcagcgcgg tctggagcag gtcggtcagc aggactttgg gatcggcgct catggcactc
    16921 ggaatgaatt cgaaacgcgc gattatagcg gatcggcacg gtccgccccg gcgaggatgt
    16981 gagcgcaggt tgcgctgcgg tgcgctcccg cccgttcccc cgcaggcgct ttcgggctaa
    17041 agtccgcggt ttgcgtctgc ccggcctgtc aacgtgcgcc tcttccgcct cgccaagatc
    17101 gtctccgtca gcctgcgctt cggcctcgac cgcatggtgc tcgacgccga cagcagcggc
    17161 cggctggcgc acgcgtggca caaggtcttc ttctggcgca ccttttccga gtcgcgcggc
    17221 gcgcgcctgc gccaggcgct ggagtcgctc gggccgatct tcgtcaagtt cggccagatg
    17281 ctgtcgaccc ggcgcgacct gctgccgccc gacctcgccg acgagctcgc cctgctgcag
    17341 gaccgcgtcc cgcccttccc gaccgagcag gcgctcgaag tgctggaagg cttctacggt
    17401 cgtccgatcg acgaggtctt cgacgacttc gagcgcacgc cggtggcctc ggcctcggtg
    17461 gcgcaagtgc atttcgcgcg cctgcccgac ggcaccgacg tcgcggtcaa ggtgctgcgc
    17521 ccgggcatcg agcgcgtcat cgagcacgac ctcgccctgc tcgaagtcgc cgcggtgctg
    17581 ctggagaaga tctggcccga aggccgccgc ctcaagccac gcgaggtggt cgcagagttc
    17641 agcaagtacc tgcacgacga gctcgacctg atgcgcgagg cggccaactg ctcgcagctg
    17701 cggcgcaact tcaccgactc ctcgctgctg atcgtgcccg aggtgtattg ggactggtgc
    17761 ggcagcaagg tcatggtgat ggagcgcatg cgcggcgtgc cgatctcgca gacgccggcg
    17821 ctgctcgccc agggcaccga tttgaaagca ctctcccgtg ccggcgtgga gatcttcttc
    17881 acccaggtct tccgcgatgg cttcttccac gccgacatgc acccgggcaa catcttcgtg
    17941 cactccgacg ggcgctacat cgcgctcgac ttcggcatca tgggcaccct taacgaggtg
    18001 gacaagaact acctcgcgac caacttcctc gccttcttca agcgcgacta ccggcgcgtg
    18061 gcgctcgccc acatcgaggc cggctgggtg ccggcgaaga cgcgggtgga cgagttcgag
    18121 gcggcgatcc gcaccgtgtg cgaaccgatc ttcgacaagc ccttgaagga catctccttc
    18181 ggcaagaccc tcctgcgcct gttccagacc gcgcgccgct tcgagatgga ggtgcagccg
    18241 cagctcgtgc tgctgcagaa gaccctgctc aacatcgagg gtctgggccg ccagctcgac
    18301 cccgagctcg atctgtggaa gaccgccaag cccttcctcg agcgctggat ggacgagcgc
    18361 atgggcgtgc gcgcgctggt gcgcggggtc aaggaagagg cgccggcctg ggccggcacc
    18421 ctgccgcagc tgccgcgcct ggtgcatcag gcgctcaccg aatcggcccg tcaccagagc
    18481 gcccagcagc agcgcctcga agagctcgcc gccgggcaac gcacccaggg ccgcctgctg
    18541 ctcttcatcg gcgccgtcgc catgggcctg ctcggcctgg agctctaccg cctgttcggc
    18601 tgacgcgcgc cgcggcattc aacgccgacg ttgcgcccat gcaataaagc gcgaacgatc
    18661 aagcccttgg cgcgcgcatc ccgccccccg cggcggcgtt ctgttggtac actcgcgccc
    18721 tcttttttcg ctcaccgcgc gcggatgcgc caacacgaaa ggcccaacca tgctgctctg
    18781 gcttgtcgtc gcctacctgg tcgtctccat cggcatcggc ctgtatgccg ccacccgggt
    18841 gcacaatgcg cgtgactaca tcaccgcagg gcgcaacctg ccgatgatcg tcgtgctggc
    18901 gatggtgttc gccacctggt tcggcgccga gaccgtgctc ggcatctccg ccaccttcct
    18961 cgaggaaggc ttccacggcc tgatctccga cccgctcggc gcctcggcct gcctggtgct
    19021 gttcggcctc gtcttcgccc gcccgttgta ccgcatgaac ctgctcacgc tgggcgactt
    19081 cttccgcgtg cgctacaacc gcacgaccga actcgtgctg tcgatctgca tcgtgctctc
    19141 ctacctgggc tgggtggcgg cgcaggtcac cgcgctcggg ctggtgttca acgtgctctc
    19201 gggcgacgcg gtgacgatga accagggcat gctgatcggc gccggcgtcg tcctcgtcta
    19261 taccctgttc ggtggcatgt ggtcggtggc gatgaccacc ttcgtgcaga tgatcgtgat
    19321 cgtgatcggc ctggtctggg tgagctggat cgccggcgac atggcgggcg gcttcgacaa
    19381 cgtgatcgcc aaggccgcgg ccgaaggcaa gttcgagttc ctgcccgcgc tggacgcggt
    19441 cgagatcgtc gcctggatcg ccgcgttcgc caccatggca ctcggctcta tcccgcagca
    19501 ggacgtgttc cagcgcgtga attcctccaa gaacgagcgc gtcgcgatct ggggcacgac
    19561 gctcggcggg gtgagctact tcttcttcgc cgcggtgccg ctcttcctcg cctacaccgc
    19621 caacatcatc gatcccggca tggtcgagcg cctgatggag caggactcgc agctcatcct
    19681 gccgacgctg atcctgcagt acatgccctt ctacgcccag gtgatcttct tcggcgcgct
    19741 gctgtcggtc atcatgtcga ccgcctccgg caccctgctc gccccctcgg tgaccttctc
    19801 cgagaacgtg ctgcgcggct tcttcccggg catgggcgac cgcgccttcc tgctcagcac
    19861 gcgcgtcacc gtcgtgctgt tcaccgcctt cgtcacctgg tacgccacca gcaccgaatc
    19921 ctcgatccac gagatggtcg agaacgccta ccgcgtcacc ctggccggcg ccttcgtgcc
    19981 gctcgccgcc ggcctgttct ggaagcgcgc caacaacctc ggcgccgcgc tggcgatcat
    20041 cctcggcctg tcgacctgga tcctcttcga gctcttcgtc cccgaaggcg acatcgagcc
    20101 ccagctctac ggcgtggcgg cgagcgcgct cggcatgctg gcgggtggac tgatcggccg
    20161 gcagccgcac caccgcccgc acatcggcgg ccaccatgcg gcggcgggca cccaccacac
    20221 gcatcgctga ccgtcccgcg cccgcccggg tgcgtgcagc ccgcaccggc ggccgatgcg
    20281 tttgatggtg atcaacgcgt cggtcgccgg tctcgtttat ggttgcggcg tttttttcct
    20341 ctggaaaccc cgctccatgt ccgtgtcttc cccgatcgaa ctcgtctgcc ccgcgggcag
    20401 cctcccggcc ctcaagacgg ccatcgatca cggcgccgac tgcgtctatc tcggtttcaa
    20461 ggacgccacc aacgcacgca acttttccgg gctcaacttc gatccggcgc aggtgcgcga
    20521 gggtgtcgcc tatgcgcacg cacgcaagcg caaggtgctg ctggcgctca acacctatcc
    20581 ccagaccgac aactggtcgt cctggacaca ggcgatcgac cgcgccgccg actacggcgt
    20641 tgacgcggtg atcctcgccg atcccgggct catggcctac gccgcaaaga cccacccgca
    20701 gctgcgcctg cacctgtcgg tgcagggctc ggcgaccagc tacgaggcga tcaactggta
    20761 ccgcgaacgc ttcggcatcc agcgcgcggt gctgccgcgc gtgctgtcga tggcccaggt
    20821 cgagagcgtg gtcgccaaca ccccggtcga gatcgaggtg ttcggcttcg gcggcctgtg
    20881 cgtgatggtc gagggccgct gcgcactgtc ggcctacgcc accggcgagt cgcccaactg
    20941 caacggcgcc tgctcgccgg gcaagcacgt gcgctgggaa gacaccccgc gcggcatgga
    21001 gacgcggctc aacggcatcc tcatcgaccg cttcgccgag ggcgagcgcg ccggctaccc
    21061 gacgctgtgc aaggggcgct tcgaggtcaa cgacgagacc tactacgcgc tcgaggagcc
    21121 caccagcctc aacaccctcg agctgctgcc agagctcgcc cgcatcggca tccgcgcgat
    21181 caagatcgag ggccgccagc gcagcccggc ctacgtcgcc caggtcacca aggtgtggcg
    21241 tgccgcgctc gacaagttga acagcggcgg cgccgcaggt ttcagcgtgc tgccggcgtg
    21301 gatggccgag ctcaacaagg tctccgaagg ccagagccac accctcggcg cctactaccg
    21361 cccgtggaag tgagcctcgc gccgaccgcc cgcgccgtcc cgacccgatc cgatcgccac
    21421 cgcgcggagc aggcgcccgc agcgcgcccg cccgcggcgt cgagcccccc atccgaatga
    21481 gaaccgcccg atgaaactcg ccctcggccc cctgctctac tactggcccc accagagcgt
    21541 gctcgacttc tacgccgacc tggccgaagc cccgctcgac agtgtttacc tcggcgagac
    21601 ggtgtgctcg cgccgccacg aactgcgcct ggacgactgg ctggaggtcg gcaaggttct
    21661 cgccgccgcc ggcaaggagg tggtgatcag ctcgcaggcc ctgatcgaat ccgagtccga
    21721 cctcaagacc ctgcgccgca tcgtccggca gcacgacttc cgcgtcgagg ccaacgacat
    21781 gggcgcggtg cgcctgctcg tcgaggccgg cgtggacaac tggatcgccg gccccacgct
    21841 gaacatcttc aaccccacga cgctgcagct catgatcgac tcgggcgcga cgcgctgggc
    21901 ggtcgccccc gagatgtcgg gcgcggcgct cgccgaggtg cgcgccgcgc tcgccaggcc
    21961 gatcgagacc gaagtcttcg cctacggtcg cctgccactg gcgcactcgg cgcgctgctt
    22021 caccgcccgt cacttcaacc tgcaaaagga cacctgcgag ttccgctgcc tgtcgatcat
    22081 ggacgggatc acgctgcgca cccgcgaggg cgcgcccttc ctgacgctca acggcgtgca
    22141 gacccagtct gcacgggtgc acaacctgct cggcgacctg cccgccgtgc gcgccagcgc
    22201 cgaggtgctg cgcatcagcc cgcaaggggc gcacactcgc cagatcgtcg aactcttccg
    22261 cgccgcgctc gacgagcagc gcggtccggc ggacgcgctc gccgccagcc gcgagtggat
    22321 ggtcgaggag ccctgcaacg gcttctggca cggccgcgcc ggcgtcgaac agtacgtcgc
    22381 cgcctgaccc ctttccgacc tgctgcaaac ccggagtccg cacatgcccg cactgcctgc
    22441 cctgccccgc ccaccgctgc cttcgcactt gctgcccggc agcctgcgcc agcgcatcgg
    22501 cgaacgcctc gccaccctgc gcatccccga cttcaccgtg cccgccccgg tggcgaagct
    22561 ggtgtcgcgc ctgccccagc agccgccgac ccacgtgctc acgctcgcgc tcaacctcgc
    22621 gctcgaccgc atcctgccgc gcgacaccct cgaaccgctg agcggccgcc acctgcagat
    22681 gcgtgtgctc gacgccgggc tgacgctcga cttcaccctc ggcgagcgcg gcttccgccg
    22741 tgccaccccg gccgcgggcg ccagaccgga cctgatcctc tccgccacca cgcgcgactt
    22801 catggcgctg gcactgcgcg aggaagaccc cgacaccctt ttcttcagcc gccgcctgcg
    22861 catggaaggc gacaccgatc tcggcctgct ggtcaagaac acgctcgacg cggtggactg
    22921 ggacgccctc aaggcgaagc tgctcggccg ccacggctga ccccctgnnn nnnnnnnnnn
    22981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23041 nnnnnnnnnn nnnnnnnnnn nnnnnnnaag ccgcgaaccg gcccctccgg ccaccgccgc
    23101 gcatcgaccg cagcgcgtcc gtccgcatcc gcggtaagct cgcggtccgc tgcccgacgt
    23161 ttgccgccat gccctccgaa ttcgacctga tccggcgcca cttcacccgc cccgcgcgcc
    23221 acaccgagct cgccgtgggc gacgacgccg ccctgctcgc gccgcgcccc ggcatgcggc
    23281 tggcggtgtc caccgacatg ctggtggccg gcacccactt cttcgccgac accgacccgg
    23341 aagggctagg ctggaagacg ctcgcggtca acgtctccga cctcgccgcc atgggcgccg
    23401 agccgcgctg ggccttcctc gcgctcgcgc tgccgcaggc ggacgaggcc tggatcgctg
    23461 ccttcgcgcg cggcttcttc gcctgcgccg acgccttcgc gatcgacctc gccggcggcg
    23521 acaccacgcg cggaccgctg acgctttcag tgaccatcgt cggcgaggtc ccggccggcg
    23581 ccgcgatcac ccgtggcgga gcgcgcgcgg gcgacgactt gtggatctcg ggccagcccg
    23641 gccgcgccgc gctcgggctc gcggcgctgc gcggcgaaac cggcctggac gccatccacc
    23701 acgccgaatt cgtcgacgcc ctgcaacggc cgcagccgcg cgtagcgctc gggctcgcgc
    23761 tgcgcagcct ggcgagcgcg atgctggacg tctccgacgg cctgctcggc gatctgcgac
    23821 acctcctcga atgctcgggc acgggcgcca tcgtcgaggt cgcagcgctg ccgctcgccg
    23881 cgctcgccgc cagcggcgcc gatcccgcgc tgtgccggcg ctgcctgctc gcgggcggag
    23941 acgactacga gctgctgttc gcggccgcgc cccgacaccg tgccgagatc gatgccttgt
    24001 cggcccgatt cgacctcccc ctgcatcgca tcggcgaatt cacccccgca cgcgagcgcc
    24061 tgctgctgcg cgaggccgac ggcggcctgg cggccccggc cgcatgcggc tacgatcact
    24121 tcgcctgagg cccgatccgg cccacctctc gaaacgacca tgcgccccac cgcccgcctg
    24181 ctgatgagcc accccgccca cttcatctcg ctgggcttcg gcgccgggct gtcgccgtgg
    24241 gcgccgggca cggtcggcac cctgctcggg tgggcgatct acccactgat ccgcaccccg
    24301 gtgtcggagt tcgtcttcct cgcactgctc gccagcttct tcctcgccgg cgtgctcgcc
    24361 gccgagcgca ccgggcaagc gctgggggtg ccagaccacg gcggcatcgt ctgggacgag
    24421 atggtggcga tctggctggt gctgctgttc acccccgccg ggctgctgtg gcaggcgatc
    24481 gcggtcgcac tgttccgctt cttcgacatc gtcaaaccgc ctccggtgcg ctgggccgac
    24541 cgcagcttca agggcggctt cggggtcatg ttcgacgacc tcgtcgccgc cggctacacc
    24601 ctgctggcgc tcgcggtgct ggttcacctg ctggggagtt gagaccatga ccgatcacga
    24661 actcgacgcc ctcgcgcgca gggtgggcga agccctcgcc aaccgcaggc tgtggctggc
    24721 caccgcggag tcctgcaccg gcggctggat cgccgaggct gtcaccgcga tcgccggcag
    24781 ctcgggctgg ttcgaccgcg gcttcgtgac ctactcgaac gacgccaagg ccgacatgct
    24841 cggcgtgcgc gccacgacgc tggagacgca cggcgcggtc agcgaagcca ccgtgggcga
    24901 aatggccgcg ggcgcgctcg accacagccg cgccgacctc gcggtggccg tgtccggggt
    24961 cgcgggaccg ggtggaggca gccccgccaa gccggtcggc acggtctgcc tcgcttgggc
    25021 acggcgcgac ggccccaccg gggtcgtcac cgtccatctc gacggcgacc gcgccgcggt
    25081 gcgccggcag acggtgatcc tcgccctgca aggcgtactc gaacactgcg tctgaggggc
    25141 gcagggcgca cggggccggg aaatccgaac caccaccctt gccacttcgc cggacgcagc
    25201 cccgcgccgg aaatccccat gacatgtttg ctgtaattgc atacaggctc tacacttacg
    25261 gcatcaggcc tgcaccccgc atgtctgcaa tctttgtgcc ataatcaggc aatcaaacag
    25321 gacgcacttc catggacgaa aacaaggcca aagccctctc cgcggcgctg tcgcagatcg
    25381 aaaagcagtt cggcaagggt tcgatcatgc gcatgggcga cggcagcatc gagcgcgaca
    25441 tccagaccgt ctccaccggc tcgctcggac tcgacatcgc gctcgggctc ggcggtctgc
    25501 cgcgcggtcg ggtggtcgag atctacggcc ccgaatcctc cggcaagacc acgctcaccc
    25561 tgcaggtcat cgccgagatg cagaagctcg gcggcacc
//
LOCUS       DAOQNN010000008        24790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_630_length_24790_cov_9.235456, whole genome shotgun sequence.
ACCESSION   DAOQNN010000008 DAOQNN010000000
VERSION     DAOQNN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 24790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24790
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_630_length_24790_cov_9.235456"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PKV97_01290"
     CDS             complement(<1..589)
                     /locus_tag="PKV97_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04537.1"
                     /translation="MLLADKRIELDARPTARRLAPVEVVLIGHCPSLDGRARWDRLAR
                     DPAVLGEIAALGFGAEVERLRALAAGATAGEAGDEALLALYRLPIPIRSLGHFQALFP
                     EAFAVACGYRSTLAGDAAWLPMAVQDFFATDERGFEDTRALWVIRVPEAEGFAAFLPR
                     PDASLAEPAGLGAFERALLVERAGILALPDLERLLI"
     gene            complement(594..1052)
                     /locus_tag="PKV97_01295"
     CDS             complement(594..1052)
                     /locus_tag="PKV97_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNB04538.1"
                     /translation="MAVFRETPYSAFNFLVDLEPGQGEEVQAGFSEVSGLNAEITVAE
                     YRAGNDRVNYVRKVPGIHKAGDVTLKRGVIGAQNLYEWLELGRTGKLAQVKRDIVVKL
                     QSEDRAGTVVSWKLRGAMPIKWTGPTLTAKGGGDIAIEELVLSVETIEQE"
     gene            complement(1070..3244)
                     /locus_tag="PKV97_01300"
     CDS             complement(1070..3244)
                     /locus_tag="PKV97_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HNB04539.1"
                     /translation="MPEYLAPGVFVEETSFRNKSIEGVGTSVAALVGPTRSGPLRGLP
                     EVLTSYADFERIYGDAHDLAFGADGKAVLNHTAHAARAFFDNGGKQLFVARVMQGVNG
                     LAADAAGVWPGAAMKADAGNTLRFFSRFPGRMGRYTLELRWRDSENLLRSETAASARA
                     GALYFLEARGVTLAAKAAGPIDDAAFPVDLRAIVRLDGANLAIQDGRATIVSRADEDA
                     PAALAANQITSLIPAQLPAGARLTRVFARPPASGALAAGTPAELRLAEVVDLAAFTGG
                     AGWGSRKAVRGTLDAAGEVFTATPALNPGLAEAVTLPLAALAAAPGAARALFVLRSFD
                     VDVRNGGKDGEVIRTYPGLTTAATGPTSLAAGLAVTPDRRAEALSSPVACRLAANATD
                     GAILAALLSICAPAARDPGEASLDEPRYLIELEGGSDGDEPGAIDYAGEADETKGSTG
                     LKALEDVEDVAIVMTPAAAGSAEDVHKAVVMEVQKHCRRMRYRIGIVDARAGQSLGEL
                     RAFAGNFDDSRLALYHPWVVIPDPTRTRRDITVPPAGFIAGVYARTDVDRGVHKAPAN
                     EIVMGALRFDQQINAFQQELLNPNGINCLRSFAGRGHRVWGGRTLSSDPEWKYVNVRR
                     YFLYLERSIEKSTQWAVFEPNGEALWANIRSSVEDFLFAEWRNGRLLGATPKEAFFVR
                     CDRSTMTQNDIDNGRMVCLVGVAALKPAEFVIFRIGQKTADA"
     gene            complement(3289..4056)
                     /locus_tag="PKV97_01305"
     CDS             complement(3289..4056)
                     /locus_tag="PKV97_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04540.1"
                     /translation="MIVETLVLHASDFIEWEDASGAQGGARRPVCVRAFRIQFDDAPA
                     DLELVHKNTGTALWRRVPAGLRQVIDGEADAGALQPPDEPPYALSGALLDPAGHYLPR
                     RFALDAGRRTAHKLRLYRSPLGTRFGRAGGLVGRTALEDGSAVPWALLALRVTPPLAP
                     PFDFVAQSDARGEFRLTLERLPALTKDAPAASYPGLLEVRAAAAGTNPDTLPVARIRG
                     NGSGDGAFKTQLAVDIAPGRIATLASPGREALVLRFP"
     gene            complement(4053..4649)
                     /locus_tag="PKV97_01310"
     CDS             complement(4053..4649)
                     /locus_tag="PKV97_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HNB04541.1"
                     /translation="MASHLGILSALRALRERLAIRMAVRLGGNPQVEILGSQTLAGKP
                     QANTDTLGLYLHRISVDPFSRNRHLPPQPGRHLPRPELPVNLHVLLVAWCTHTEQEIE
                     YLAAAIQIIGAGLVLESADMSLADPDWGSEDVVQVLPEEMSTEDLMRLWDSLPDDYRL
                     SAPYLVKTVRLAPDLEREEGPPVKTLVFPMHVPDGSAR"
     assembly_gap    4840..4939
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4940..5407)
                     /locus_tag="PKV97_01315"
     CDS             complement(<4940..5407)
                     /locus_tag="PKV97_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04542.1"
                     /translation="MHELYTRADPAYTGRATVPVLRDRSRHTIVKNESADILQILNSD
                     FGAMAHNDIDLRPLSADPNMMAYLRRLLALPAFAGSTRADHIKAGYYSIRALNPTGIV
                     PAGPLLDDLCAGSLSALPRARRDDALPFGPGRRRVRQAARAGSSSRPGRNRRHR"
     gene            complement(5525..6424)
                     /locus_tag="PKV97_01320"
     CDS             complement(5525..6424)
                     /locus_tag="PKV97_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB04543.1"
                     /translation="MDRFVEMQTFCAVVDAGSFVGAAEALGLSKATTSRYVGELETRL
                     GVRLLHRTTRRLSLTEDGERFYARSREVLAGLEEAEAELHSRSGAARGLLRINAPVTF
                     GIRHLAPLWGAFRDLHPQVRLEVTLADRTVDLVEEGFDIAIRIAALPSSTLVCRQLAR
                     TRIVLCAAPAYLAAHGAPRLPADLASHTVIAYSYWSGGDEWQFDGPDGRVSVRTRPAV
                     HTNSGDTCRAMALAGQGVILQPGFLVAEDLAAGALVELMPAYRSVTLGIYALYPTRKF
                     VPPKVRALVDFLAERFAAPPPGW"
     gene            6704..7531
                     /locus_tag="PKV97_01325"
     CDS             6704..7531
                     /locus_tag="PKV97_01325"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNB04544.1"
                     /translation="MQMLPTLFVSHGAPTFALDPGLAGANLATLGRRLPRPHAVLVVS
                     PHWMTRQPQVTLSLRPATIHDFGGFDATLYTLQYPAEGAHLAARRALELLQAAGLPAT
                     PNADRGLDHGAWVPLSHLYPKADIPVFQVSLPEWLDADGAYDYGRVLAPLAGEGVLIV
                     GSGSLTHNLYEFRLGDPRAQAYAAEFAHWVRDAVLAGDHPRLRQALEIAPHARRAHPT
                     AEHYLPLLVAAGAAAQAVPASVIEGGIVHGVLSMDSFLFGADLAAMKGADANQALPD"
     gene            7541..8161
                     /gene="ypfH"
                     /locus_tag="PKV97_01330"
     CDS             7541..8161
                     /gene="ypfH"
                     /locus_tag="PKV97_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HNB04545.1"
                     /translation="MSDQDLIVQQASAPAARLILLFHGVGSRPEDLVPLGRLLAEADP
                     GAWVVSVRSPDPSDLGGGWQWFSVRGVTEDSRPARIAEAMPSFVRQVRDWQARAGVDA
                     AATTLVGFSQGAIMALESTQLPETVAARVISIAGRFALPPRVAPANTQVHLLHGEQDP
                     VIDARYSLMAAETMQALGAAVSVELFPDLGHGVDARVAGRVKALAA"
     gene            complement(8316..8391)
                     /locus_tag="PKV97_01335"
     tRNA            complement(8316..8391)
                     /locus_tag="PKV97_01335"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8356..8358),aa:His,seq:gtg)
     gene            complement(8435..8511)
                     /locus_tag="PKV97_01340"
     tRNA            complement(8435..8511)
                     /locus_tag="PKV97_01340"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8475..8477),aa:Arg,seq:tct)
     gene            complement(8539..8615)
                     /locus_tag="PKV97_01345"
     tRNA            complement(8539..8615)
                     /locus_tag="PKV97_01345"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8579..8581),aa:Pro,seq:tgg)
     gene            complement(8687..9985)
                     /locus_tag="PKV97_01350"
     CDS             complement(8687..9985)
                     /locus_tag="PKV97_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04546.1"
                     /translation="MMGPGLSYDDTPPFSAPIRFFLTAPLFGVAAGLTLIFGGEILVS
                     RWTPGALAITHLFAAGFMLQVMLGALLQVMPVVAGASMPAPLRIAGITHLAMALGAAG
                     LAFGLGAGAPGVLLGGAVLLVGGLFVFLSAAALGLSRGARTGNNTRTPRDLRMAFAGL
                     VVTAVLGFSLVLVLTRGVALPVPLPTVVNLHAGWGWMGWAAVLLAAASWVVVPMFQIT
                     AAYPQRFTTLWAPAVTATLVLWTLAEYLAIDAARFIAILALGLLGAGYAGTTLYLQAH
                     SRRSKPDTPFLAFREAMYSALAGVLVLAISLWSDADCWPILAGVLILHGGFGGTITAM
                     LYKIVPFLAWLHLTQAGLKAPNMKKLLPDTPIRRQLRVRTTSLATLCVAVFVPVLAPL
                     AGVVLAVEFGWLFANLLRVVRARRDAAKNAGPRPGSHASA"
     gene            complement(9982..10200)
                     /locus_tag="PKV97_01355"
     CDS             complement(9982..10200)
                     /locus_tag="PKV97_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNB04547.1"
                     /translation="MSGRVVDARGLEPPEPFERAMEGLMALGKDEEMLLILDRMPHPL
                     LRILDRDGYRHAEAFRDDGAVEIRIRQR"
     gene            complement(10197..10655)
                     /locus_tag="PKV97_01360"
     CDS             complement(10197..10655)
                     /locus_tag="PKV97_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNB04548.1"
                     /translation="MNTISELMTHDHRDCDGIFARAEELASDGDWADAAEALKKFADA
                     LNAHFDAEESTLFPAFEQATGMTQGPTMVMRHEHQNMRDTLAALQYALDKQDADDFAG
                     EAETLLIMMQQHNMKEESVIYPMLDSRLPGDEQERLSASVGKHLGEARAA"
     gene            10910..12106
                     /locus_tag="PKV97_01365"
     CDS             10910..12106
                     /locus_tag="PKV97_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB04549.1"
                     /translation="MFVPFALGYYLSYLLRTVNAVISPRLTDELGLSAASLGLLTSAY
                     FLAFGAAQIPVGIALDRFGPRRVEAALLLLAALGSALFAMADSLGALAGARAMVGLGV
                     SACLMAALKGFTLWYPPERQSSMIGFIMASGSLGALTASVPLAAVLPVLGWRGAFWII
                     AAVSLFAAVLALRALPDSPAARSEASLAAALRVVAGIFAAPAFLRHAAASVFFVGGFM
                     ALQSLWVVPWVMNVNGFDIAGAATVLVALNLGNLAGQLAVGVFGVRLARAGVQPLGLL
                     RAGYGMMLVVQALILWSDLPVLPLWTAFGLLTAANSQIYLSAARAFPPALFGRVSTAL
                     NLMAFAGAFCVQWGFGIGLDLMRGMGVELARALAWGFGALLALQALAYLPLLGRPAAR
                     PSKGEA"
     gene            12276..16076
                     /locus_tag="PKV97_01370"
     CDS             12276..16076
                     /locus_tag="PKV97_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNB04550.1"
                     /translation="MSEPTTDSTVQPPRRKGLRRTLWTVAILLALSAAAYLAVPPVAR
                     HYAQKLLGETLGREVVVERVLINPFRLTAEVGGLRIMEADGSAEALGFESLRANLELE
                     SLLRKGIVLHELALDKPRVHVALEAEGRHNWTDVLERIAALGGEEPAEDTPPTLFSIG
                     NIRISDGLVRVDDRVRGITHELSELGVGVPFVSNLPVRVDVFVQPSLSAKLNGDALLL
                     NARTKPFAESQETILDVSLKEFDLTPWIAYLPIEPRFRLPSALLTTNLELSFSQTVEG
                     APVLSLRGPLQVHKLVLQDREGAPVATADEVELEFADVQPMIGRWHFTRLRLARPELD
                     LVRLKDGGLNVLGLISPPAAEGARKAKGKAAKGGKAAAQVAQKAASGPAAGAAQADAG
                     VTTPAAPADGKTVADARDATPAGADFLLALTRIRDGVVRFEDRGLAEPFRARIEAINL
                     DMRDLATEGELPAEIRLDYVTDAGEKFTHEDVLRLAPFEYSGSLQFESIRLPRYAPYL
                     AAAMPGASIGEGSLSGGAQYRVTLGKDGAPAVQVSAEALALRDFALLLKDAKTPALSL
                     PALDVRSATVDLAGRSVRIGGVELQGAAVSAVRTKDGEFDLVRALVPPPAKGAAPAPA
                     AKGKSDPDWVIAVDKFALRGASVRLDDRSVARPVLTTLEGIDFELDGFSTAKGDAIQF
                     KLDSMLNKRGKVAASGPLTIAPFKADLALDLRNVDLLPLQPYVLEQTKIAISRGNLTT
                     KGRLKLDTDRRGKLLASFSGDVGVANFASVDRLNATDFLRWRTLAIGGIDARLEPLSL
                     GVKRIALDDFYTRLILDSQGRLNLREIGGVKGESEAAAKPAAAVAHTKEVEQGEMQGG
                     AAADGQRTSPIAPPGPPPPPVRIDRIELKRGNVAFSDRFVRPNYDANLTGLAGTITGF
                     STSSDKLARVEIVGKVDKSAPLSITGELNPFRQDAHLDILASVKDFELTGLSSYSGKY
                     VGYGIAKGKLSAELNYKIIDRQLTATNRIFLDQLTFGDAVDSPDAVNLPVQLAVSLLK
                     NSRGEIDLNLPVSGTLDDPQFSVFGLVMKMLFNLIGKAITSPFALLGSALGGGEELSQ
                     LDLAAGTATLGEAEQGRLKTLAQALVDRPTLRLDIVGRADPASDLDGLRQAALTNAVR
                     AQKLKAMIAKGQAAPSIEEVEVGEAEYAELLRKAYRAADFKKERNLVGMVKDIPVPEM
                     EALMRANAKVGDDDLLALARARAQVVRDWLAGEGGVPGERVFVLEPKVEALGEGGEVQ
                     FSLR"
     assembly_gap    16333..16432
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16436..17005)
                     /gene="sufT"
                     /locus_tag="PKV97_01375"
     CDS             complement(16436..17005)
                     /gene="sufT"
                     /locus_tag="PKV97_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HNB04551.1"
                     /translation="MGDHYGETDSRVLQRDCAAVSVPWGKAETLPEGSYALVTQRLGG
                     SITVMCGGNLYRIDERNADALGLEPQLSAAPPADADGEQTAERVEQAAWAQLATCYDP
                     EIPIDIVNLGLVYACTAEPLADGRFRIAAAMTLTAPGCGMGTLIADEARDKLLQIPGV
                     ADAEVTLVWDPPWSREMMSEAARLEMGMF"
     gene            complement(17024..17488)
                     /locus_tag="PKV97_01380"
     CDS             complement(17024..17488)
                     /locus_tag="PKV97_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNB04552.1"
                     /translation="MSGMQDSLRELYQEVIFDHNRNPRNYRPLPAANHHADGHNPLCG
                     DQLTVYLQVEDGIVRDASFVGHGCAISTASASLMTEAVKGKPVAEVEALFRDVHALLT
                     EGREAADPARDFGKLEVLSGVKEFPARVKCATLAWHTLHNALVGEHETAHTE"
     gene            complement(17485..18768)
                     /locus_tag="PKV97_01385"
     CDS             complement(17485..18768)
                     /locus_tag="PKV97_01385"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNB04553.1"
                     /translation="MNAPCDPGAQTPILDLAADFPILSRPVHGRRLAYLDNGATTQKP
                     AAVIEAEARFYRESNANIHRGVHWLSQHATELYDGARATVQRFLNAARADEIVFTRGT
                     TEAINLVAQSWGRPRLAAGDEILLSTMEHHSNIVPWQLVCEQTGAVLKVIPVRDNGEL
                     DMAAFAGLLGERTRLLAITHVSNALGTVNPVAEMTRRAHEMGAIVLVDGAQAVAHQAV
                     DVQAIGCDFYAFSGHKLYGPTGIGALYGRTELLRHMPPWQGGGDMIRTVAFDNTTFAP
                     PPQRFEAGTPNIAGAIALAAAIDYVQGVGLARIHVHEQALLDYGTRALAAIPGVRLVG
                     TAERKAGILSFLVDGIHPHDLGTILDAEGVAIRAGHHCAMPLMTRFGIPGTARASLGL
                     YNGLADLDALVAAIHKAQELFGTRGGTASGNGRVA"
     gene            complement(18765..20129)
                     /gene="sufD"
                     /locus_tag="PKV97_01390"
     CDS             complement(18765..20129)
                     /gene="sufD"
                     /locus_tag="PKV97_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNB04554.1"
                     /translation="MSAVDFWLGRQRDTAADLPGADLPWLVRLREDAIDRFADEGWPT
                     TRRENWRHTSLAFMAQQALAVREAVAPEAVVADLRARYRAALGTAQDPARKAVESAGD
                     DGHWVVFVDGHFAPALSAIGGLPGGVRIGSLADALGQTPEAVEAAFGQAGDGEAPAAL
                     NAALAADGAWIRLGRGVAVEPPIHLVFLTATVADQHLRNLVVAEPGAQATIVEHYPAG
                     AAGSTLTTAVTRVLAAQDSHVTHLKLQQEAPEAIHLATIEAEQARGAVFASHSLSFGA
                     RLARNDIRTRLAGEGAEVLLNGLYHADGRRHVDHHTRIDHARPQGTSREFYRGLIDGS
                     ARGVFTGRILVAQDAQRTDAMQRCDNLLLSRLAEADARPELEIYADDVKCAHGATVGQ
                     LDEDALFYLRSRGVDAVHARQLLTYAFAAEVLDRIAHASLRAFGRAALLERLPDGARL
                     EELL"
     gene            complement(20126..20911)
                     /gene="sufC"
                     /locus_tag="PKV97_01395"
     CDS             complement(20126..20911)
                     /gene="sufC"
                     /locus_tag="PKV97_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNB04555.1"
                     /translation="MLKINNLHAAIDGKAILKGLNLEVADGEVHAIMGPNGSGKSTLA
                     QVLAGRETFTVTDGSVDWNGADLLALPAEERARAGLFLAFQYPVEIPGVSNAYFLKAA
                     VNAVRRHRGLPEYDAMDFLARVKAEMKAVGMKEEFLYRSVNEGFSGGEKKRNEVLQMA
                     LLEPRLAVLDETDSGLDIDALKIVAEGVNRLRSPARSMIVITHYQRLLDYIVPDKVHV
                     LSQGRIVRSGGRELALELEEHGYGWIDAAQGGKVGGAGTGARS"
     gene            complement(21060..22568)
                     /gene="sufB"
                     /locus_tag="PKV97_01400"
     CDS             complement(21060..22568)
                     /gene="sufB"
                     /locus_tag="PKV97_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNB04556.1"
                     /translation="MMNAPTSAPTSTPSSASIASPARRGDAIGAFLEQDYAAGFETTL
                     DTDTVPKGLSEDIIRIISARKGEPEWLLDWRLEAYRHWLTMSPPTWAAVQFPPVDFQD
                     IVYYSAPKSKKDGPMSLDEVDPELLRTFEKLGVPLHERARLAGVAVDAVFDSVSVATT
                     FKKELGAHGVIFCSFSEAVKEHPELVRKYLGTVVPPGDNFYAALNSAVFSDGSFVFIP
                     KGVRCPMELSTYFRINARDTGQFERTLIIAEEGASVSYLEGCTAPMRDENQLHAAVVE
                     LIALDDAKIKYSTVQNWYPGDKDGKGGIYNFVTKRGDCRGARSHISWTQVETGSAITW
                     KYPSVILRGDDSVGDFHSVALTNNLQQADTGTKMIHMGRNTRSTILSKGISAGRGSNT
                     FRGLVKITPKAEGARNYTQCDSLLIGDRCAAHTFPTIEVRHPTAHVEHEATTSRIGED
                     QLFYAMQRGISAEDAVSMIVNGFCREVFKELPMEFAVEAQKLLGVSLEGSVG"
     gene            complement(22568..23083)
                     /locus_tag="PKV97_01405"
     CDS             complement(22568..23083)
                     /locus_tag="PKV97_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HNB04557.1"
                     /translation="MLRISKLTDYGTLILTHMAGHPDRLHSAAGLAEALGLGLPTVSK
                     VLKTLGRHELVSSQRGAHGGYALARPPDRITVADVIDALEEQAFGLTECSSSSGVCAI
                     ESDCRIRENWMRINTVVRQALQAVNIADMVHPVHGAAFPAPHTTEHRMHGPARSAAAC
                     LPAARADEQEI"
     assembly_gap    23310..23409
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23410..23770
                     /locus_tag="PKV97_01410"
     CDS             <23410..23770
                     /locus_tag="PKV97_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04558.1"
                     /translation="RGLEARRFSWERRKSRSALQSPAQGFRGASRWRGGAARLSFHQG
                     ARLRRHDDCEDFDTMEPVGGCSYVIRLCSGELRRWRFEGVDQRGFGWWLDEETGLGFS
                     DASLMYAWEILERADDG"
     gene            23763..24314
                     /locus_tag="PKV97_01415"
     CDS             23763..24314
                     /locus_tag="PKV97_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNB04559.1"
                     /translation="MAEPWTLRLALAYPASLVVPCAGLVVLAMSSQPTAAAGPGLAAV
                     LVVAGLAAWTLIEYVLHRWMLHGIEPFQRWHLAHHRHAGVTIRVPVLFSVLLVLTVAG
                     LPALISGGSAYAAPLSAGMLLGNLLQEAVHHRLHDTRPAGRWLEARRRLHGFHHFCDE
                     RRGYGTVTDLWDRVFGTLPPPHR"
     gene            complement(24397..24771)
                     /locus_tag="PKV97_01420"
     CDS             complement(24397..24771)
                     /locus_tag="PKV97_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNB04560.1"
                     /translation="MTDPASRAPLLAGALLAALGVALGAFGAHALRGALDAAAQGWWQ
                     TAVQYQMWHALGLLAIGAARVQGGSLPASLLAGGTLIFCTTLYAMALGAPRWLGAVTP
                     IGGSLMIAGWLVLVWRVARPRR"
BASE COUNT         3511 a         8245 c         8871 g         3863 t
ORIGIN      
        1 ggatcagcag ccgctcgagg tcggggagcg cgaggatgcc ggcgcgctcc accagcagcg
       61 cgcgttcgaa ggcgcccagc ccggcgggct cggccaggct cgcgtcgggg cgtggcagga
      121 aggcggcgaa gccctcggcc tcgggcacac ggatcaccca cagcgcgcgc gtgtcctcga
      181 agccgcgctc gtcggtggcg aagaaatcct gcaccgccat cggcagccac gccgcatcgc
      241 ccgccagggt gctgcggtag ccgcaagcga ccgcgaaggc ctcggggaag agcgcctgga
      301 agtggccgag cgagcggatc gggatcggca gccgatacag ggcgagcaag gcctcgtcgc
      361 cggcttcgcc cgcggtggcg cccgccgcga gggcgcgcag gcgctcgacc tccgcgccga
      421 agcccagcgc cgcgatctcg ccgagcacgg cgggatcgcg cgcgagcctg tcccagcgcg
      481 cacgcccgtc gagcgagggg caatggccga tcagcacgac ctcgaccggc gccagtcgcc
      541 gcgccgtggg acgtgcatcg agctcgatgc gcttgtcggc gagcagcacc gcctcactcc
      601 tgctcgatgg tctcgaccga gaggaccagt tcctcgatgg caatgtcgcc gccgcccttg
      661 gcggtgagcg tcgggccggt ccacttgatc ggcatcgcgc cgcgcagctt ccagctcacc
      721 acggtgccgg cgcgatcctc ggattgcagc ttgacgacga tgtcgcgctt gacctgggcg
      781 agcttgccgg tgcgtccgag ctcgagccac tcgtagaggt tctgcgcgcc gatcacgccg
      841 cgcttgaggg tcacgtctcc cgccttgtgg atgccgggga ccttgcgcac gtaattcacg
      901 cggtcgtttc cggcacggta ctcggcgacc gtgatctcgg cgttgaggcc cgagacctcg
      961 gagaagccgg cctgcacctc ttcgccttgc ccgggttcga ggtcgacgag gaagttgaat
     1021 gccgaatacg gggtttcgcg aaacacggcc atgacgatct ctccttgtct caggcgtcgg
     1081 cggtcttctg accgatgcgg aagatgacga attcggcggg cttgagcgcg gcgacgccga
     1141 cgaggcacac catgcggccg ttgtcgatgt cgttctgggt catcgtcgag cggtcgcagc
     1201 gcacgaagaa ggcctccttc ggcgtggcgc ccagcaggcg accgttgcgc cattcggcga
     1261 agaggaagtc ctccacgctg gaacggatgt tggcccacag tgcctcgccg ttcggctcga
     1321 acaccgccca ctgggtggat ttctcgatcg agcgctcgag atagaggaag tagcgccgca
     1381 cgttgacgta cttccactcg ggatcgctgg agagcgtgcg tccgccccac acgcggtggc
     1441 cgcgtccggc gaaggagcgc aggcagttga tgccgttcgg attgaggagc tcctgctgga
     1501 aggcgttgat ctgctggtcg aagcgcagcg cacccatgac gatctcgttc gcgggcgcct
     1561 tgtgcacgcc gcggtcgaca tcggtgcgcg catacacgcc ggcgatgaag ccggcgggcg
     1621 gcaccgtgat gtcgcgccgg gtgcgggtcg ggtcggggat caccacccag gggtggtaga
     1681 gcgcgaggcg ggagtcgtcg aagttgccgg cgaaggcgcg cagctcgccg aggctctgcc
     1741 cggcgcgcgc gtccacgatg ccgatgcggt agcgcatgcg ccggcagtgc ttctgcacct
     1801 ccatgacgac cgccttgtgc acgtcctcgg cgctgcccgc ggctgcgggc gtcatcacga
     1861 tggcgacgtc ctcgacgtcc tcgagcgcct tcagtccggt gctgcccttg gtctcgtcgg
     1921 cttcgccggc gtagtcgatc gcgcccggct cgtcgccgtc gctgccgccc tcgagctcga
     1981 tcaggtagcg cggttcgtcg agcgaggcct cgcccgggtc gcgcgcggcg ggggcgcaga
     2041 tggacagcag cgcggcgagg atcgcgccat cggtcgcgtt cgcggcgagg cggcaggcga
     2101 ccggcgagct cagcgcctcg gcgcgacggt cgggtgtgac cgcaagcccg gcggcgaggc
     2161 tggtcggtcc cgtggcggcg gtcgtcaggc cggggtaggt gcggatcact tcgccgtcct
     2221 tgccgccgtt gcgcacatcg acgtcgaagc tgcgcagcac gaacagcgcg cgcgcggcac
     2281 cgggcgccgc ggcgagtgcg gcgaggggca gcgtgacggc ttcggcgagg cccggattga
     2341 gcgcgggtgt ggccgtgaag acctcgcccg cggcatccag tgtgccgcgc actgccttgc
     2401 ggctgcccca gcccgcaccg ccggtgaagg cggcgagatc gacgacctcg gccaggcgca
     2461 attcggccgg cgtgcccgcc gccagtgcgc cggatgcggg cggacgcgcg aacacgcggg
     2521 tgagcctcgc gccggcgggc agctgggccg ggatcaggct ggtgatctgg tttgccgcga
     2581 gcgccgcggg ggcatcctcg tcggccctcg acacgatcgt ggcgcgcccg tcctggatcg
     2641 ccaggttggc gccgtcgagc cggacgatgg cccgcaggtc gaccgggaag gccgcgtcgt
     2701 cgatcggacc ggctgccttc gcggcgagcg tcacgccccg cgcctcgagg aagtagagcg
     2761 cgcctgcgcg ggcacttgcc gcggtctcgc tgcgcagcag gttctcgctg tcgcgccagc
     2821 gcagctcgag ggtgtaccgt cccatccggc ccgggaagcg gctgaagaac ctcagcgtgt
     2881 ttccggcgtc ggccttcatg gcggcaccgg gccacacgcc ggccgcgtcc gccgcgaggc
     2941 cgttgacgcc ctgcatgacg cgggcgacga agagctgctt gccgccgttg tcgaagaagg
     3001 cgcgtgccgc gtgcgcggtg tggttgagca cggccttgcc gtccgcgccg aaggccaggt
     3061 cgtgcgcgtc gccgtagatg cgttcgaagt ccgcgtagct cgtcagcacc tcggggagac
     3121 cgcggagcgg gccgctgcgg gtggggccca ccagcgcggc gacgctggtg ccgaccccct
     3181 cgatggattt gttgcggaag ctcgtctctt cgacaaagac gcctggagcg aggtactcgg
     3241 gcatgtcgct tctccttcgt tgccggtgag gcctgtccac ccggcgggtc agggaaaccg
     3301 caggacgagg gcttcccgtc ccgggctggc cagggtggcg atgcgcccgg gcgcaatgtc
     3361 caccgcgagt tgtgtcttga atgcgccgtc gccgctgccg ttgccgcgga tgcgcgcgac
     3421 ggggagggtg tcggggttgg tgccggcggc ggcggcgcgc acctccagca ggccggggta
     3481 gctcgccgcc ggggcgtcct tggtgagcgc cgggagccgc tcgagcgtca gccggaactc
     3541 gccgcgcgcg tcgctctggg cgacaaagtc gaaggggggg gccagcggcg gggtcacgcg
     3601 gagcgcgagc agggcccagg gcactgcgct gccatcctcg agtgcggtgc gtccgacgag
     3661 cccgccggcg cgaccgaagc gcgtgccgag cggcgagcgg tacagccgca gcttgtgagc
     3721 ggtgcggcga ccggcatcga gcgcgaaacg acgcggcagg tagtgccccg cggggtcgag
     3781 cagcgctccg ctgagcgcat agggcggctc gtccggaggc tgcagcgccc ccgcgtccgc
     3841 ttcaccgtcg atcacctgcc gcagcccggc cggcacgcgg cgccacaggg ccgtgccggt
     3901 gttcttgtgg accagctcga gatcggcagg cgcgtcgtcg aactggatgc ggaacgcgcg
     3961 cacgcacacc ggccgccgtg ctcccccttg ggcgccgctc gcgtcttccc attcgatgaa
     4021 gtcgctggca tgcagcacga gggtctcgac gatcatcgcg cgctcccgtc gggcacgtgc
     4081 atcgggaaga cgagggtctt caccggcggt ccctcctcgc gctcgagatc gggggcgaga
     4141 cgcacggtct ttaccaggta gggggcggac agccggtagt cgtccggcag gctgtcccac
     4201 agccgcatca ggtcctcggt gctcatctcc tccgggagca cctgcacaac gtcctcggac
     4261 ccccagtccg gatccgcgag gctcatgtcg gcgctctcga gcacgagccc ggcgccgatg
     4321 atctggatcg cggcggcgag gtattcgatc tcctgctcgg tgtgcgtgca ccaggccacc
     4381 agcagcacgt gcagattgac cggaagttcc gggcggggaa ggtggcgtcc cggctgcggc
     4441 ggaaggtggc ggttgcggct gaagggatcg accgagatgc gatgcaggta gagccccagc
     4501 gtgtcggtgt tggcctgcgg cttgccggcg agcgtctgcg aacccaggat ctcgacctgc
     4561 gggttgccgc cgaggcgcac ggccatgcgg atcgccaagc gttcccgcaa tgcccgcaag
     4621 gccgacagga ttccgagatg cgacgccacg cttacctcgc ttcgaggccg gagccttggg
     4681 gggacggcgt tgccgggtgc agatcaccga tcaggaaggg accggcctgt tctgcgattc
     4741 actctgaaac gtggcacatc gcgcgaagcg cgctaaagga cgaaatgtaa gtaaatgttt
     4801 tcagagcggc ctcgcccggc cgggacctgc atccgggcgn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnna cggtgacgtc ggtttcgccc tgggcggctt gacgatcctg
     4981 ctcgagccgc ttgccggacc cggcggcggc ccggaccaaa aggcagggcg tcatcgcgcc
     5041 gtgctcgggg aagcgcacta aggctgcccg cgcagagatc gtcgaggagc ggccctgccg
     5101 gcacgatgcc cgtcgggttc agcgcccgga tggagtaata cccggccttg atgtgatcgg
     5161 ctcgcgtgct gccggcaaac gcgggcagcg ccagcaagcg gcgcaggtag gccatcatgt
     5221 tcggatcggc ggagagcggg cgcaggtcga tgtcgttgtg tgccatggcg ccgaaatcgc
     5281 tgttcaggat ctgcaggatg tcggcagact cgttcttgac gatggtgtgg cgcgaccggt
     5341 cccgcagtac gggcacggtt gcacgtcccg tgtaggccgg gtccgcgcgg gtgtacaact
     5401 cgtgcatgtg atgcgcagcc ttgacgtcgt cgaggtcggt gccgagctct ccgctgaagg
     5461 cccagccctg tccggcggat ggactgttcc gccgagcgca acgaccgcgg cgatctgcgc
     5521 ggcctcacca gcccggcggc ggcgcggcga agcgttcggc gaggaagtcg accagcgcgc
     5581 gcaccttggg cgggacgaac ttgcgcgtcg ggtacagagc atagatgccg agcgtgacgg
     5641 atcgatatgc gggcatcagc tccaccagcg cgcccgccgc cagatcttcg gcgaccagga
     5701 atccgggctg caggatcacc ccttggccgg cgagtgccat cgcgcggcag gtgtcgccgc
     5761 tgttggtgtg tacggcgggt ctcgtgcgca cgctcacgcg cccgtccggt ccgtcgaact
     5821 gccactcgtc gcctccggac cagtagctgt aggcgatgac cgtgtggctg gcgaggtctg
     5881 ccggcaaccg gggcgcgcca tgggctgcca gataggcggg cgcggcgcac agcacgatgc
     5941 gcgtgcgggc gagctgccgg cagacgaggg tggaactggg cagcgcggcg atccggatcg
     6001 cgatatcgaa gccttcctcg acgagatcga cggtacggtc cgccagcgtc acctcgagcc
     6061 gcacctgcgg gtgcagatcc cggaacgcgc cccacagggg cgcgaggtgc cggatgccga
     6121 aggtgaccgg ggcgttgatg cgcaacagcc cgcgtgccgc gccactgcgc gaatgcagct
     6181 cggcttccgc ttcctcgagc ccggcgagca cttcccggct gcgggcgtag aagcgttcgc
     6241 cgtcctcggt caatgacagg cgccgggtgg tgcggtggag caggcgcaca ccgagtcgcg
     6301 tctccagttc gcccacatag cgcgaggtgg tcgccttcga caagccgagc gcctcggccg
     6361 cgcccacgaa gctgccggca tcgaccaccg cgcagaaggt ctgcatctcg acaaatcggt
     6421 ccattgtctc ttcatgtgga acagtttctc gtctggatgg ctgtttatct caatcgtgat
     6481 ggcccgtaaa gtgttgccat ggtcagttca tcgcaacagg acgccagacc agcacaaggc
     6541 gacggaaccc gcggagaaac accatgacgc ctcgcccgct cgagcgcctg ttgcccacct
     6601 acgccggctg ggccgcgcac gcgctgtcgt cccgggcccc cgggggcccg ggacgacagc
     6661 ggccggtcgg cccgcgtcat cgccgtccac ctggagacct ctgatgcaaa tgctgcctac
     6721 cctgttcgtc tcgcacggcg cgcccacctt cgcgctcgac cccggcctcg ccggcgcgaa
     6781 tctcgccacg ctcggccgcc ggctgccgcg tccgcacgcg gtgctggtgg tgtcgccgca
     6841 ctggatgacg cgccagccgc aggtcacgct gtcgcttcgg cccgcgacca tccacgattt
     6901 cggcggcttc gatgcgaccc tctacacgct gcagtacccg gccgaaggcg cccatctcgc
     6961 tgcgcgccgt gcgctcgaac tgctgcaggc ggcgggtctg cccgccaccc cgaatgccga
     7021 tcgcggcctc gaccacggtg cctgggtgcc cctgtcgcac ctttacccca aggccgacat
     7081 cccggtgttt caggtatcgc tgccggaatg gctcgacgcc gatggcgcct acgactacgg
     7141 gcgggtgctc gcgccgttgg caggcgaagg cgtgctgatc gtgggctcgg gcagcctgac
     7201 ccacaacctg tacgagttcc gcctcggtga tccacgcgcc caagcctatg cagcggagtt
     7261 cgcgcactgg gtgcgggacg cggtgctggc gggcgaccac ccgcgcctgc gccaggcgct
     7321 tgagatcgca ccgcacgccc gccgtgcgca tcccaccgcc gagcattacc tgccgctgct
     7381 ggtggccgcc ggcgcggcgg cgcaggccgt gccggcgagc gtcatcgaag ggggcatcgt
     7441 gcatggcgtg ctgtcgatgg attccttttt gttcggtgcc gacctcgccg cgatgaaggg
     7501 tgccgacgcg aaccaggcat tgcccgactg aggcgcgacc atgtccgatc aggatctcat
     7561 cgttcaacaa gcgtccgccc cggcggcgcg cttgatcctg cttttccatg gtgtgggatc
     7621 acgccccgag gatctcgtgc cgctcggccg cctgctcgcc gaggccgacc cgggcgcctg
     7681 ggtggtcagc gtgcgctcac ccgaccccag cgatctgggc ggcggctggc agtggttctc
     7741 ggtacgcggg gtgaccgagg acagccgccc ggcgcgtatc gccgaggcca tgccatcgtt
     7801 cgtgcgccag gtgcgcgact ggcaggcgag ggcaggggtg gatgccgccg ccaccacgct
     7861 ggtcggcttc tcgcagggtg cgatcatggc gctggagtcc acccagttgc cggaaacggt
     7921 cgccgcgcgc gtcatctcga tcgccggccg ctttgccttg ccaccgcgcg tggcgccggc
     7981 gaacacgcag gtgcatctgc tgcacggcga gcaggatccg gtgatcgatg cgcgctacag
     8041 cctgatggcc gccgagacga tgcaggcgct cggtgcggcc gtcagcgtcg agctcttccc
     8101 ggatctgggg catggtgtgg atgctcgtgt cgccggtcgc gtgaaggcct tggctgcctg
     8161 agtggcccgg ctctgctcca tggagcatgg atgcggtagc gaacagcgtg tccggaaacg
     8221 aaaaaaccca ccgcttcggg tgggttttca tgcttgcagc gtaccttgcg ggactcttcg
     8281 gacgctggcc gggtcccgtc gggacatcga atcgatgggg cgacataccg gaatcgaacc
     8341 ggtgacacct ggagccacaa tccagtgctc taccaactga gctaatgccg ccactgtggg
     8401 accccggaaa accggactcc cgaaaaactt ttcctggcct gcccggcagg gatcgaacct
     8461 gcaacccccg gcttagaagg ccggtgctct atccaattga gctacgggca gcttccggcg
     8521 ggcagtcggg atgtgagctg gtcggggtgg agggattcga acccccgaca tcttgctccc
     8581 aaagcaagcg cgctaccgga ctgcgctaca ccccgagagc cgcgaactat acagatgcct
     8641 ttttcgttcg tcaaacaaaa ttcgcgcgtc gcaaaaaaaa ccggaatcac gcgctggcgt
     8701 ggcttccggg gcgcggcccg gcgtttttcg ctgcatcgcg gcgggcgcgc accacacgca
     8761 gcaggttggc gaagagccaa ccgaactcga ccgccagcac gacgccggcg agcggggcga
     8821 gcaccggcac gaagaccgcg acgcagagcg tggcgagact cgtggtgcgc acgcgcagct
     8881 ggcggcggat cggggtgtcg ggcagcagct tcttcatgtt cggtgccttg agcccggcct
     8941 gggtgaggtg cagccaggcg aggaagggca cgatcttgta gagcatcgcg gtgatggtgc
     9001 cgccgaagcc gccgtgcagg atcagcacgc cggcgaggat cggccagcag tcggcgtcgg
     9061 accacagcga gatcgccagc accaggacgc cggcgagcgc cgagtacatg gcctcgcgga
     9121 aggcgaggaa aggcgtgtcg ggcttgctgc gccgcgagtg tgcctgcagg tacagcgtgg
     9181 tgcccgcgta gccggcgccg agcagcccga gtgcgaggat ggcgatgaag cgcgcggcat
     9241 cgattgcgag gtattcggcg agggtccaca acacgagggt ggcggtgacc gccggtgccc
     9301 acagcgtggt gaagcgctgc gggtaggcag cggtgatctg gaacatcggt accacgaccc
     9361 agctcgcggc ggcgagcagc actgcggccc agcccatcca gccccagccg gcgtgcaggt
     9421 tcaccacggt cggcagcggc accggcaggg cgacgccgcg cgtgagcacg agcaccaggc
     9481 tgaagccgag gaccgcggtc acgacgaggc cggcgaaggc catgcgcaga tcgcgcgggg
     9541 tgcgggtgtt gttgcccgtg cgtgcgccgc ggctgaggcc cagcgcggcg gcggagagga
     9601 acacgaagag tccgcccacg agcagcacgg cgccgccgag caggacgccg ggtgcgccgg
     9661 cacccagtcc gaaggcgagc cccgcggcgc cgagcgccat cgccaggtgg gtgatgccgg
     9721 cgatgcgcag cggcgcgggc atgctcgcgc ccgccaccac cggcatgacc tgcagcaacg
     9781 cgccgagcat cacctgcaac atgaagccgg cggcgaagag gtgcgtgatg gcgagtgcgc
     9841 cgggcgtcca ccgcgagacg aggatctcgc cgccaaagat cagcgtcagc cccgccgcca
     9901 cgccgaacag cggggcggtg aggaagaagc ggatcggtgc cgagaacggc ggcgtgtcgt
     9961 cgtaggagag gcccggcccc atcatcgctg gcggatccgg atctcgaccg cgccgtcgtc
    10021 gcggaaggcc tcggcgtggc ggtagccgtc gcgatcgagg atgcgtagca gcgggtgcgg
    10081 catgcggtcg aggatgagca gcatttcctc gtccttgccg agggccatca ggccctccat
    10141 ggcacgctcg aagggctcgg gcggctcgag gccgcgggca tcgacgacac gtccgctcat
    10201 gctgcgcggg cctcgccgag gtgcttgccg accgaggccg acaggcgctc ctgctcgtcg
    10261 ccgggcaggc ggctgtcgag catcgggtag atcacgctct cttctttcat gttgtgctgc
    10321 tgcatcatga tcagcagcgt ctcggcctcg ccggcgaagt cgtcggcgtc ctgcttgtcg
    10381 agggcgtact gcagggcggc cagggtgtcg cgcatgttct ggtgttcatg gcgcatcacc
    10441 atggtgggac cttgcgtcat gcccgtcgcc tgctcgaagg cgggaaagag ggtgctctcc
    10501 tcggcgtcga agtgggcgtt gagcgcgtcc gcgaacttct tcagcgcttc ggcagcgtcg
    10561 gcccagtcgc catcggaggc aagctcctcg gcgcgggcga agatgccgtc gcagtcgcgg
    10621 tggtcgtggg tcatgagttc ggaaatggta ttcatcggag tctcctctct ggcgggtgta
    10681 tcgaattctc cgcagggggc gacaaacggc attgatgcaa gtcaagggca tcccggctgc
    10741 gttggactga caactgtttg tcgtgcatca aagtccgcat cgcttgggcg gcacggcgct
    10801 tgcaggcaca atgcgcgctt tttcgcaggg gacgcgggtg gtccggacga ggcggtcggg
    10861 cggggcggag agcggctgca agggcggctc gacgagcggc tgggtacggg tgttcgtgcc
    10921 gttcgcgctc ggctactacc tgtcctacct gctgcgcacg gtcaacgcgg tgatttcgcc
    10981 gcggctcacc gacgagctcg gcctgtcggc ggccagcctg ggcctgctga ccagcgcgta
    11041 tttcctcgcc ttcggcgcgg cgcagattcc ggtcgggatc gcgctcgacc gcttcggtcc
    11101 gcggcgggtg gaggccgcgc tgctgttgct cgcggcgctc ggcagcgcgc tgttcgccat
    11161 ggccgactcg ctcggcgcgc tcgcgggcgc acgcgccatg gtgggcctgg gggtgtcggc
    11221 ttgcctgatg gcggcgctga aggggttcac gctgtggtat ccgcccgagc gccagagctc
    11281 gatgatcggt ttcatcatgg cctcgggctc gctcggcgcg ctcaccgcca gcgtgccgct
    11341 cgcggcggtg ctgccggtgc tgggctggcg cggggcgttc tggatcatcg ccgcggtgtc
    11401 cctgtttgcc gcggtgctgg cgctgcgcgc gctgcccgat tcgccagcgg cgcgtagcga
    11461 ggcctcgctg gcggccgcgc tgcgcgtcgt ggcgggcatc ttcgccgcgc ccgccttcct
    11521 gcgccatgcg gcggcctcgg tgttcttcgt cggcggcttc atggcgctgc agagtctgtg
    11581 ggtcgtgccg tgggtgatga acgtgaacgg cttcgacatc gccggtgcgg cgacggtact
    11641 ggtggccctc aacctcggca acctcgccgg acagctcgcg gtgggcgtgt tcggcgtgcg
    11701 cctggcgcgc gccggagtcc agccgctcgg cctgctgcgc gcgggctacg gcatgatgct
    11761 ggtggtgcag gcgctgatcc tgtggtccga cctgccggtg ctcccgctgt ggaccgcatt
    11821 cggcctgctc accgcggcca actcgcagat ctacctgtcg gcggcgcgcg cctttccgcc
    11881 cgcgctgttc ggccgcgtca gcaccgcgct caacctgatg gcgttcgccg gcgccttctg
    11941 cgtgcagtgg ggcttcggca tcggcctcga tctcatgcga ggcatgggtg tggagcttgc
    12001 gcgcgcgctg gcctggggct tcggagcctt gctcgcgctg caggcgctcg cctatctgcc
    12061 gctgctcgga cgcccggcgg cgaggccgtc caagggcgag gcttgaatgc ccgcatggac
    12121 cgtctcaccg tgttgcgtgt gcacagcttc cttacccgag cgccacaaag cggcgcaaaa
    12181 taacgcccgg ccgggctccg gcgcatgcca ggcgggctcc cgccgcatga tgtccgggca
    12241 gttccggcga cgaattcatc ctgatggagt ttcccatgtc tgaacccacg accgactcga
    12301 ccgtgcagcc gcctcggcgc aaggggctgc gtcggacgtt gtggacggtc gcgatcctgc
    12361 tcgcgctctc ggccgccgct tacctcgccg tgccgccggt ggcgcggcac tacgcgcaga
    12421 agctgctcgg cgagacgctc gggcgcgagg tcgtcgtcga gcgcgtgctg atcaacccct
    12481 tccgcctaac tgccgaggtg ggcggactgc gcatcatgga ggccgatggc agcgccgagg
    12541 cgctcggctt cgaatcgctg cgcgccaacc tcgagctcga gtcgctgctg cgcaagggca
    12601 tcgtgctgca cgagctcgcc ctcgacaagc cgcgcgtcca tgtcgcgctc gaagccgagg
    12661 gtcgccacaa ctggacggac gtgctcgagc gcatcgccgc gctcggtggc gaggaacccg
    12721 ccgaggacac gccgccgacg ctgttctcga tcggcaacat ccgcatcagc gacggccttg
    12781 tgcgcgtcga cgaccgcgtg cgcggcatca cccacgagct ctccgaactg ggcgtcggcg
    12841 tgcccttcgt ctccaacctg ccggtgcggg tggatgtctt cgtgcaaccc tcgctctcgg
    12901 ccaagctcaa cggcgacgcg ctgctgctca acgcgcgcac caagcccttc gccgaatcgc
    12961 aagaaaccat cctcgacgtc tcgctgaagg agttcgacct cacgccctgg atcgcctacc
    13021 tgccgatcga gccgcgcttc cgccttcctt cggcgctgct gacgaccaat ctcgagctct
    13081 cgttcagcca gacggtggag ggtgcgccgg tgctctccct gcgcggtccg ctgcaggttc
    13141 acaagctggt gctgcaggat cgcgagggcg cgccggtcgc gaccgcggac gaggtggagc
    13201 tcgaattcgc cgacgtgcag ccgatgatcg ggcgctggca cttcacccgg ctgcgcctgg
    13261 cacgtccgga gctcgacctg gtgcggctga aggacggcgg gctcaacgtg ctcgggctga
    13321 tatcgcctcc ggccgccgaa ggcgccagga aggcaaaggg caaggctgcg aaagggggca
    13381 aggccgccgc gcaggtcgcg cagaaggcgg cttccggacc tgccgcgggt gcagcccagg
    13441 cagacgcagg cgtcaccacg ccggccgccc cggcggacgg caagacggtg gccgatgccc
    13501 gcgacgcaac cccggccgga gcggatttcc tgctcgcgct cacgcgcatc cgcgacgggg
    13561 tggtgcgctt cgaggaccgc ggcctggccg agcccttccg cgcgcgcatc gaggcgatca
    13621 acctcgacat gcgcgatctc gccaccgaag gcgagctgcc ggccgagatc cgcctcgatt
    13681 acgtcaccga tgccggcgag aaattcaccc acgaggacgt gctgcgcctg gcgcccttcg
    13741 agtactcggg cagcctgcag ttcgagtcga tccgcctgcc gcgctatgcg ccctacctgg
    13801 cggcggcgat gcccggcgcc tcgatcggcg agggcagtct cagcggcggt gcgcaatacc
    13861 gcgtgacgct cggcaaggac ggcgcgccgg cggtgcaggt tagcgccgag gcgctcgcac
    13921 tgcgcgattt tgcgctgctg ctgaaggatg cgaagacgcc ggcgctgagc ctgccggcgc
    13981 tcgacgtgcg cagcgccacg gtcgacctcg ccgggcgcag cgtgcgcatc ggcggggtgg
    14041 agctccaggg ggcggcggtc tccgccgtgc gcaccaagga tggcgagttc gacctcgtga
    14101 gggcgctcgt gccgccgccg gcgaagggtg cagctcccgc gccggcggcc aagggcaagt
    14161 ccgatcccga ctgggtgatc gcggtggaca agttcgcgct gcgcggcgcc tcggtgcggc
    14221 tggacgatcg cagcgtggcg cggccggtgc tcaccacgct cgagggcatc gacttcgagc
    14281 tcgacggctt ctccaccgcc aagggcgacg ccatccagtt caagctcgac tccatgctga
    14341 acaagcgggg caaggtggcg gcctcggggc cgctgacgat cgcgccgttc aaggccgatc
    14401 tcgccctcga tctacgcaac gtcgacctgc tgccgctgca gccctacgtg ctcgagcaga
    14461 ccaagatcgc gatctcgcgc ggcaacctca ccaccaaggg ccggctcaag ctcgacaccg
    14521 accgccgcgg caagctgctc gcgagcttca gcggcgacgt cggggtggcg aacttcgcct
    14581 cggtcgatcg cttgaacgcg accgacttcc tgcgctggcg cacgctcgcg atcggcggta
    14641 tcgacgcgcg ccttgagccc ctctcgctcg gcgtcaagcg catcgcgctc gacgatttct
    14701 acacccgcct gatcctcgac agccagggcc gcctgaacct gcgcgagatc ggcggcgtca
    14761 agggtgagtc cgaagcggcc gcgaagccgg cggctgccgt cgcgcacacg aaggaggtcg
    14821 agcagggcga gatgcagggt ggggcggccg ccgacggcca gcgtacttcc ccgatcgcgc
    14881 ccccaggccc gccgcccccg ccggtgcgca tcgaccgcat cgagctcaag cgcggcaacg
    14941 tcgccttcag cgaccgcttc gtccgcccca actacgacgc caacctgacc gggctcgccg
    15001 gcaccatcac cggcttctcc acgagctcgg acaagctcgc cagggtggag atcgtcggca
    15061 aggtggacaa gagcgcgccg ctgtcgatca ccggcgagct caaccccttc cgccaggatg
    15121 cccacctcga catcctcgcc tcggtgaagg atttcgagct caccgggctg tcgagctact
    15181 ccggcaagta cgtcggctac ggcatcgcca agggcaagct gtcggcggag ctcaactaca
    15241 agatcatcga ccgccagctc accgcgacca accgcatttt cctcgaccag ctcaccttcg
    15301 gtgacgcggt ggacagcccg gatgcggtga accttccggt gcagctggcg gtgtcgctgc
    15361 tcaagaacag ccgcggcgag atcgacctca acctgccggt gagcggcacg ctcgacgatc
    15421 cgcagttcag cgtgttcggc ctggtgatga agatgctgtt caacctgatc ggcaaggcga
    15481 tcacctcgcc ctttgcgctg ctcggctcgg cgctcggcgg cggcgaggag ctctcgcagc
    15541 tcgacctcgc cgcgggcacg gccaccctgg gcgaggcgga gcagggcagg ctgaagacgc
    15601 tcgcgcaggc gctggtcgat cgccccacct tgcgcctgga catcgtcggc cgtgccgacc
    15661 ccgcctccga cctcgacggc ctgcgccagg cggcgctgac caacgcggtg cgcgcgcaga
    15721 agctcaaggc gatgatcgcc aagggtcagg ccgcgccttc gatcgaggag gtcgaggtgg
    15781 gcgaggcgga gtatgccgag ctgctgagga aagcctaccg tgccgccgac ttcaagaagg
    15841 agcgcaacct ggtcggcatg gtcaaggaca ttcctgtgcc cgagatggag gcgctgatgc
    15901 gcgccaacgc gaaggtcggt gacgacgact tgctggctct ggcgcgcgca cgtgcgcagg
    15961 tcgtgcggga ttggctggcg ggcgaggggg gcgtgcccgg cgagcgcgtc ttcgtgctcg
    16021 agccgaaggt ggaggcgctc ggcgagggcg gcgaggtgca gttctcgcta cgctgatcgg
    16081 ccgtgaaaat cgcgacttgc gtcgctccta cgggagggtg tccaggcgtg acgccttccc
    16141 gacgcggagg ttttccacgc gcgtgccgtt tcctccatgc gtgcgatgtc ttttccacgc
    16201 tcgatgcttt ctccccgcgc gacgttttcg caggagcgac gcaagtcgcg atgccgcagg
    16261 agcgacgcaa gtcacgatgc cgcaggagcg acgcaagtcg cgatgccgta ggagcgacgc
    16321 aagtcgcgat gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccctcaga
    16441 acatccccat ctccagccgc gcggcctcgc tcatcatctc gcggctccac ggcgggtccc
    16501 agaccagggt gacctcggca tcggcgacgc cgggaatctg caacagcttg tcgcgcgcct
    16561 cgtcggcgat cagggtgccc atgccgcagc cgggcgccgt gagggtcatc gccgcggcga
    16621 tgcggaacct gccgtccgcc agcggctctg ccgtgcatgc atagaccagg cccaggttca
    16681 cgatgtcgat cgggatctcg gggtcgtagc aggtggcgag ttgcgcccac gccgcctgtt
    16741 cgacgcgctc tgcggtctgc tcgccgtccg cgtcggccgg cggggctgcc gacaactgcg
    16801 gctcgaggcc gagcgcgtcg gcgttgcgct cgtcgatgcg gtagaggttg cccccgcaca
    16861 tcaccgtgat cgagccgccc aggcgctggg tcaccagcgc gtaggacccc tcggggagcg
    16921 tctccgcctt gccccagggc acgctcaccg ccgcgcagtc gcgctgcagc acgcgggaat
    16981 cggtttcgcc gtagtggtcg cccatgatgt gctcctcgag gtcttattcg gtgtgtgcgg
    17041 tttcgtgctc gccgacgagc gcgttgtgca gggtgtgcca ggcgagcgtg gcgcacttga
    17101 cccgcgcggg aaactccttg accccggaga gcacttccag cttgccgaag tcgcgcgccg
    17161 ggtcggcggc ctcgcggcct tcggtgagca gcgcgtggac gtcgcggaac agcgcctcga
    17221 cctctgcgac cggcttgccc ttgaccgcct cggtcatcag cgacgccgaa gcggtggaga
    17281 tcgcgcagcc gtggccgacg aagctcgcgt cgcgcacgat gccgtcctcg acctgcaggt
    17341 acaccgtgag ctggtcgccg cacagcgggt tgtggccgtc ggcgtggtga ttggcggcgg
    17401 gcagcgggcg gtagttccgc gggttgcggt tgtgatcgaa gatcacctcc tggtacagct
    17461 cgcgcagcga atcctgcatc ccgctcatgc cactctcccg ttgccagacg ccgtgccgcc
    17521 gcgcgtgccg aacagttcct gcgccttgtg gatcgccgcg accagcgcgt cgaggtcggc
    17581 gaggccgttg tagaggccga gcgaggcgcg cgcggtgccg gggatgccaa agcgcgtcat
    17641 cagcggcatc gcgcagtggt gcccggcgcg gatcgccaca ccctcggcgt cgaggatggt
    17701 gccgaggtcg tgcgggtgga tgccgtcgac caggaaggac aggatgccgg ctttgcgctc
    17761 cgcggtgccg accaggcgta ccccggggat ggcggcgagc gcgcgcgtgc cgtagtcgag
    17821 cagggcctgc tcgtggacgt ggatgcgcgc gagcccgacg ccttggacgt agtcgatcgc
    17881 cgcagccagc gcgatcgccc cggcgatgtt gggcgtgccg gcctcgaagc gctgcggcgg
    17941 cggggcgaag gtggtgttgt cgaaggcgac ggtacggatc atgtcgccgc caccctgcca
    18001 ggggggcatg tggcgcagca gctcggtgcg gccgtagagc gcgccgatgc cggttgggcc
    18061 gtagagcttg tggcccgaga aggcgtagaa gtcgcatccg atcgcctgca cgtccaccgc
    18121 ctggtgtgcc accgcctgcg cgccgtccac gagcacgatc gcgcccatct cgtgcgcgcg
    18181 ccgcgtcatc tcggccaccg gattgaccgt gccgagcgcg ttcgacacat gggtgatcgc
    18241 cagcagccgc gtgcgctcgc cgagcaggcc cgcgaaggcc gccatgtcga gctcgccgtt
    18301 gtcccgcacc ggaatcacct tgagcaccgc gccggtctgc tcgcacacga gctgccaggg
    18361 cacgatgttg gagtggtgct ccatggtgct gagcaggatc tcgtcaccgg ccgcgagccg
    18421 tggccggccc cagctctgcg ccaccaggtt gatcgcctcg gtggtgccgc gggtgaacac
    18481 gatctcgtcg gcgcgcgcgg cgttgaggaa gcgctgcacc gtggcgcggg cgccgtcgta
    18541 gagttcggtg gcgtgctgcg acagccagtg cacgccgcgg tggatgttgg cgttggactc
    18601 gcggtagaag cgcgcctcgg cctcgatcac cgcggcgggc ttctgcgtgg tggcgccgtt
    18661 gtccaggtag gccaggcggc gaccatgcac cggccgcgac aagatgggga agtcggcggc
    18721 aaggtccagg atgggcgtct gcgcgccggg gtcacacgga gcgttcacag cagttcctcc
    18781 agtcgggcgc catcgggcag gcgttcgagc agcgcggcgc ggccgaacgc gcgcagcgac
    18841 gcgtgggcga tgcggtcgag cacctcggcg gcgaaggcgt aggtcagcag ctggcgtgcg
    18901 tgcaccgcat cgacgccccg gctgcgcagg tagaagaggg cgtcctcgtc gagctggccg
    18961 acggtggcgc cgtgggcgca cttgacgtcg tcggcgtaga tctcgagctc ggggcgggcg
    19021 tcggcctcgg ccaggcggga cagcagcagg ttgtcgcagc gttgcatggc gtcggtgcgc
    19081 tgggcgtcct gggccaccag gatgcgcccg gtgaacaccc cgcgcgcgct gccgtcgatc
    19141 aggccgcgat agaactcgcg gctggtgccc tgcgggcggg cgtggtcgat gcgggtgtgg
    19201 tggtcgacat ggcggcggcc gtcggcgtgg tagaggccgt tcagcagcac ctcggcgccc
    19261 tcgccggcga gccgggtgcg gatgtcgttg cgcgccaggc gggcgccgaa ggacagcgag
    19321 tgcgaggcga acaccgcgcc gcgggcctgc tcggcttcga tcgtcgccag gtggatggct
    19381 tcgggcgcct cctgctgcag cttgaggtgg gtgacgtggc tgtcctgcgc ggcgagcacg
    19441 cgggtgacgg cggtggtcag cgtgctgccg gcggcgccgg ccgggtagtg ctcgacgatc
    19501 gtcgcctgcg cgccgggctc ggcgacgacg aggttgcgca ggtgctggtc ggcgacggtc
    19561 gcggtgagga agacgaggtg gatgggcggc tccacggcga cgccgcgacc caggcggatc
    19621 caggcgccgt cggcggcgag cgcggcgttg agcgcggcgg gggcctcgcc gtcgccggcc
    19681 tggccgaagg cggcctcgac cgcctcgggg gtctgcccga gcgcatcggc caggctgccg
    19741 atgcgcacgc cgccgggaag cccgccgatg gcggacagcg cgggggcgaa gtgcccatcc
    19801 acgaagacca cccagtggcc gtcgtcgccg gcggactcca cggccttccg cgcggggtcc
    19861 tgtgctgtgc ccagcgctgc ccggtagcgc gcgcgcaggt ccgccacgac cgcctcgggc
    19921 gcgaccgcct cgcgcaccgc cagcgcctgc tgcgccatga aggcgagcga ggtgtgtcgc
    19981 cagttctcgc gccgcgtcgt cggccagcct tcgtcggcga agcggtcgat ggcgtcctcg
    20041 cgcaggcgga cgagccaggg caggtcggcg ccgggcagat cggcagcggt gtcgcgctgg
    20101 cgtccgagcc agaagtcgac cgcgctcatg accgtgctcc cgtgcccgcg ccgccgacct
    20161 tgccgccctg cgccgcgtcg atccagccgt agccgtgctc ttccagctcg agggcgagct
    20221 cgcgcccgcc cgagcgcacg atgcggccct gcgacagcac gtgcaccttg tccggaacga
    20281 tgtagtcgag caggcgctgg tagtgggtga tgacgatcat cgagcgcgcg ggcgagcgca
    20341 ggcgattgac gccctcggcg acgatcttca gcgcatcgat gtccaggccc gagtcggtct
    20401 cgtccagcac cgcgagcctg ggctcgagca gcgccatctg cagcacctcg ttgcgcttct
    20461 tctcgccgcc ggagaagcct tcattcaccg agcggtagag gaattcctcc ttcatgccga
    20521 ccgccttcat ctcggccttc acccgcgcga ggaagtccat cgcgtcgtat tccggcagcc
    20581 cgcggtggcg gcgcaccgcg ttgactgcgg ccttgaggaa gtaggcgttc gacacgccgg
    20641 ggatctcgac cgggtactgg aaggcgagga agagcccggc gcgcgcgcgc tcctcggcgg
    20701 gcagggcgag caggtcggcg ccgttccagt cgacgctgcc gtcggtgacg gtgaaggtct
    20761 cgcgtccggc cagcacctgg gcgagcgtgc tcttgcccga gccgttggga cccatgatcg
    20821 cgtgcacctc gccgtccgcg acctcgaggt tcaggccctt gaggatcgcc ttgccgtcga
    20881 tcgcggcgtg caggttgttg attttcagca tgtttgttct ccggatttcc atctcgatgt
    20941 cgcaagtggc gcccccgtag gagcgacaca agtcgcgatc gtgcttctcg ggccttggat
    21001 cgcgacttgc gtcgctccca cgaaaaaacg cacggccctg tttccatgtg ggcgccggct
    21061 cagccgacgc tgccttcgag gctcacgccc agcaactttt gtgcttccac cgcgaattcc
    21121 atcggcagct ccttgaacac ctcgcggcag aagccattga cgatcatcga caccgcgtcc
    21181 tcggcgctga tgccgcgctg catcgcgtag aagagctggt cctcgccgat gcgcgaggtg
    21241 gtggcctcat gctcgacgtg cgccgtgggg tggcggacct cgatggtcgg gaaggtgtgc
    21301 gccgcacagc ggtcgccgat cagcagcgag tcgcactggg tgtagttgcg cgcgccctcg
    21361 gccttggggg tgatcttgac caggccgcgg aaggtgttgc tgccgcgccc ggccgagatg
    21421 cccttgctga ggatggtgct gcgggtgttg cgccccatgt ggatcatctt ggtgccggtg
    21481 tcggcctgct gcaggttgtt ggtcagtgcc accgagtgga agtcgcccac cgaatcgtcg
    21541 ccgcgcagga tcacgctcgg gtacttccac gtgatcgccg agccggtctc gacctgggtc
    21601 caggagatgt gcgagcgcgc gccgcgacag tcgccgcgct tggtgacgaa gttgtagatg
    21661 ccgcccttgc cgtccttgtc acccgggtac cagttctgca cggtggaata cttgatcttg
    21721 gcgtcgtcga gcgcgatcag ctcgaccacc gcggcgtgga gctggttctc gtcacgcatc
    21781 ggcgcggtgc agccttcgag gtaactgacg gacgcgcctt cctcggcgat gatcagcgtg
    21841 cgctcgaact ggccggtgtc gcgggcgttg atgcggaagt aggtggacag ctccatcgga
    21901 cacctcacgc ccttggggat gaagacgaag gagccgtcgg agaacaccgc ggagttgagc
    21961 gcggcgtaga aattgtcgcc cggcggcacc acggtgccga ggtacttgcg caccagctcg
    22021 gggtgttcct tcaccgcctc ggagaaggaa cagaagatca cgccgtgggc gcccagctcc
    22081 ttcttgaacg tggtcgccac gctcaccgag tcgaacaccg catccaccgc cacgccggcc
    22141 aggcgcgcgc gttcgtgcag cggcacgccg agcttctcga aggtgcgcag cagctccgga
    22201 tcgacctcgt ccaggctcat gggaccgtcc ttcttcgact tcggcgccga gtagtacacg
    22261 atgtcctgga agtccaccgg cgggaactgc accgcagccc aggtcggcgg gctcatcgtc
    22321 agccagtgac ggtaggcctc gaggcgccag tcgagcagcc attcgggttc gcccttgcgt
    22381 gccgagataa tccggatgat gtcttccgac agacccttgg gcacggtgtc ggtgtcgagc
    22441 gtggtctcga agccggcggc gtagtcctgc tcgaggaagg caccgatcgc atcgccgcgg
    22501 cgggcagggg aggcgatgga ggccgaggaa ggggtcgaag tgggggcaga ggtgggggcg
    22561 ttcatcatca gatctcctgt tcgtctgcgc gcgctgcggg gaggcaggcc gcggcgctgc
    22621 gcgcggggcc atgcatgcgg tgttcggtgg tgtgcggggc ggggaaggcg gcgccgtgga
    22681 cggggtggac catgtcggca atattcaccg cctgcagcgc ctggcgcacc acggtgttga
    22741 tccgcatcca gttctcgcgg atacggcagt cggattcgat cgcgcacacg ccgctgctcg
    22801 agctgcactc ggtgagcccg aaggcctgct cctcgagcgc gtcgatcacg tcggccacgg
    22861 tgattcggtc gggcggacgg gcgagcgcgt aaccgccatg cgcgccgcgc tggctgctca
    22921 ccagctcgtg gcggccgagc gtcttgagca ccttgctgac cgtgggcaga ccgaggccga
    22981 gcgcttcggc gaggccggcg gcgctgtgca gtcgatccgg atgcccggcc atgtgggtca
    23041 ggatcagggt gccgtaatcg gtgagtttgc tgatgcgcag catgtcgatg tgctcgcgtg
    23101 ggtggtgtcg gaattaaaag taccaaaacg gtactgtata gtcaagccgt ggcatctcga
    23161 ctgcggggac cacgacttgc gtcgcccagg tgtcccgcgc cctgcctgcc gtaggagcga
    23221 cgcaagtcgc gattgcagcg caccgggtct gcgccagcgc cgcggggcca cccgatcgcg
    23281 acttgcgtcg ctcctaccgg acgccccggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23401 nnnnnnnnnc cgggggctgg aggcgcgacg gttttcgtgg gagcgacgca agtcgcgatc
    23461 tgcgctgcaa tcccccgcgc aggggttcag gggtgcgtcg cgctggcgtg gtggtgcagc
    23521 gcggctatca ttccatcagg gcgcgcgctt gcgccggcat gacgattgcg aggacttcga
    23581 caccatggaa cccgtcggcg gctgcagcta tgtcatccgc ctgtgcagcg gcgaactgcg
    23641 ccggtggcgc tttgaaggcg tggatcaacg cggattcggc tggtggctgg acgaggagac
    23701 cggactcggc ttttcggacg cgagcctgat gtacgcctgg gaaatcctgg agcgcgccga
    23761 cgatggctga accctggacc ttgcgcctgg cgctcgccta tccggcctcg ttggtggtgc
    23821 cgtgcgccgg tctggtcgtg ctggcgatga gttcgcagcc gaccgccgct gcgggcccgg
    23881 gcctcgccgc cgtgctggtg gtggcgggct tggccgcgtg gacgctgatc gaatacgtcc
    23941 tccaccgctg gatgctgcat gggatcgagc ccttccagcg ctggcacctg gcccatcacc
    24001 gccatgccgg ggtgacgatc cgcgtaccgg tgctgttcag cgtgctgctg gtgctgacag
    24061 tggccggctt gccggcgctg atttcgggtg gcagcgcata cgcggcgccc ttgtcggcgg
    24121 gcatgctgct cggcaacctg ctgcaggagg cggtgcacca ccgcctgcac gacacccgcc
    24181 cggcggggcg ctggctggag gcgcggcggc ggctgcacgg cttccaccat ttttgcgacg
    24241 agcgccgcgg ctacggcacg gtgacggatc tttgggaccg cgtcttcggc acgctgccgc
    24301 cgccacaccg ctgaagagcg ggcgctgcaa gccgctgcgg cggctcccaa agtgcccgtg
    24361 tccttgaaag acgctttcca agctgcggcc gtgtcctcag cgccgcgggc gcgccactcg
    24421 ccagaccagc accagccagc cggcgatcat caggctgccg ccgatcgggg tgaccgcccc
    24481 cagccagcgc ggcgcaccca gcgccatcgc gtagagcgtg gtgcagaaga tcagcgtgcc
    24541 gccggcgagc agcgaagccg ggaggctgcc gccctgcacg cgcgccgcgc cgatcgcgag
    24601 cagcccgagc gcgtgccaca tctggtactg caccgcggtc tgccaccagc cttgggcggc
    24661 ggcatcgagc gccccgcgca gcgcatgcgc accgaaggcg ccgagcgcga cgccgagggc
    24721 agcgagcaga gcgccggcga ggaggggagc gcgggaggcg ggatcggtca tggagagggg
    24781 tgcgagctag
//
LOCUS       DAOQNN010000009        23890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_679_length_23890_cov_9.864191, whole genome shotgun sequence.
ACCESSION   DAOQNN010000009 DAOQNN010000000
VERSION     DAOQNN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 23890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23890
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_679_length_23890_cov_9.864191"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKV97_01425"
     CDS             complement(<1..540)
                     /locus_tag="PKV97_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNB04561.1"
                     /translation="MLAKRIIPCLDVKSGRVVKGVNFVELRDAGDPVEIARRYDEQGA
                     DEITFLDITASSDDRDIILHVVEQVAEQVFIPLTVGGGVRVVEDVRRLLNAGADKVSM
                     NTAAVNNPQLVFEAASKVGSQCIVVAIDAKQTAPGKWEVFTHGGRNNTGLDAIEWARK
                     VESLGAGEILLTSMDRDGTK"
     gene            complement(563..1312)
                     /gene="hisA"
                     /locus_tag="PKV97_01430"
     CDS             complement(563..1312)
                     /gene="hisA"
                     /locus_tag="PKV97_01430"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNB04562.1"
                     /translation="MLLIPAIDLKDGQCVRLKQGEMDDATVFSSDPGAMARHWLEAGA
                     RRLHLVDLNGAFAGKPKNGAAIRSITDVVGDDIPVQLGGGIRDLDTIEHYLDNGISYV
                     IIGTAAVKNPGFLHDACSAFPGHIIVGLDAKDGKVAVDGWSKLTGHDVVDLARKFEDY
                     GVESVIYTDIGRDGMLSGVNIEATVRLARALRIPVIASGGITDLRDIDALCAVEDEGI
                     MGAITGRAIYEGTLDFAAAQARADELEGARE"
     gene            complement(1357..1995)
                     /gene="hisH"
                     /locus_tag="PKV97_01435"
     CDS             complement(1357..1995)
                     /gene="hisH"
                     /locus_tag="PKV97_01435"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNB04563.1"
                     /translation="MTSVAIIDYGMGNLRSVAKAIEHVAPGHEVFVTSDPARVAAAER
                     VVFPGQGAMPDCMRELDARGLRPAVRAAAAEKPFLGICIGQQMLFQHSAEGDVPGLGI
                     LSGEVVRFPETGMFAADGSRLKVPHMGWNEVRQCGPHPMWDGIPDGERFYFVHSYFVV
                     PAEASLTAAETDYGVRFTSAVARANIFAAQFHPEKSAAAGLRLLANFIRWQP"
     gene            complement(2057..2644)
                     /gene="hisB"
                     /locus_tag="PKV97_01440"
     CDS             complement(2057..2644)
                     /gene="hisB"
                     /locus_tag="PKV97_01440"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNB04564.1"
                     /translation="MRQAEVTRNTLETKITVRIDLDGSGQGRLDTGVPFFDHMLDQIV
                     RHGLFDLDIHCEGDTHIDDHHTVEDVGITLGQAFAKALGDKKGLRRYGHAYVPLDEAL
                     SRVVVDFSGRPGLHYFVNYTRARIGNFDVDLAREFFQGFVNHAGVTVHIDNLRGDNAH
                     HQCETVFKAFARALRMAAEHDERAAGTIPSTKGAL"
     gene            2773..3846
                     /gene="hisC"
                     /locus_tag="PKV97_01445"
     CDS             2773..3846
                     /gene="hisC"
                     /locus_tag="PKV97_01445"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNB04565.1"
                     /translation="MSRFWSAVVHGLTPYVPGEQPKLDNLVKLNTNEHPCGPSPKVLE
                     AIRAATGDGLRLYPDPNADALKAALARHHGVRPEQIFVGNGSDEVLAHAFMALLKHER
                     ALWFPDITYSFYPVYCGLYDIQPRIVPLAEDFSIRVEDYLPRGDERAGAIIFPNPNAP
                     TGRLLALAEVERIVAANPEAVVLVDEAYVDFGGDSAIALVDRHPNLLVTRTFSKSRAL
                     AGLRVGYAVGDAALIEALERVKNSFNSYPLDRLAIAGAVASVEDEDYFRASCAQVIET
                     RARLVADLQALGFEVLPSAANFIFARHPTRDGAELTAELRRRAIIVRHFKAARIDQFM
                     RITVGTDAQCAILVAALREILAG"
     assembly_gap    3886..3985
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4099..5043)
                     /locus_tag="PKV97_01450"
     CDS             complement(4099..5043)
                     /locus_tag="PKV97_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB04566.1"
                     /translation="MKARPASAQGHGRFWVGLLPWVWMLFFFVLPFALIVNVSLSTPD
                     LAVPPYVSPFAGAAADGAPAFRPDPASYARLGGELAALGTLAADRPYLAAYANSLLLA
                     TLTTLCCLVLGYPFAYRLARAPEAMRNLLLMLVMLPFWTSFLLRVYAWMGLLDSSETG
                     LINQVLLGLGLVAEPLPLLYNTGAVLVGMVYSYLPFMVLPLYANLVKLDQRLPEAARD
                     LGARPATVFLRITLPLSSRGIVAGSMLVFIPAVGEFVIPDLLGGGSVMTIGRSIWDDF
                     GPNQDWPMAAAVAVLMVAMLIAPIVLLQRHGRGAGRDE"
     gene            complement(5040..6149)
                     /locus_tag="PKV97_01455"
     CDS             complement(5040..6149)
                     /locus_tag="PKV97_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB04567.1"
                     /translation="MAGERVHLRIESLGKRFGDTWAVREVSLDIGEREIFALLGSSGC
                     GKSTLLRMIAGLETPSCGRILLDGEDLAAVPAHRRPTNMMFQSYALFPHMTVAGNVAF
                     GLRQLRPRMARAAIAARVDEMLELVQMRAYAGRRPHALSGGQQQRVALARSLAREPRL
                     LLLDEPLAALDKRIRQQTQFELVGLIRRVGVTCILVTHDQEEAMTMADRIGILSEGGL
                     VQVGTPGEIYERPNCRFSASFIGETNLFHGRIEGDRLVGGEFPQPIRFVATVALQQGG
                     EAWLSLRPECVRVHHRAAGDACVPEQPDDNAAPAEVAEIAYLGGRSIYHLRLPGGGSM
                     IASVPAAHWGEAAPPARGEPVRVSWPAPDGVLLLR"
     gene            complement(6214..7302)
                     /locus_tag="PKV97_01460"
     CDS             complement(6214..7302)
                     /locus_tag="PKV97_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB04568.1"
                     /translation="MTLKFGWMAVLALAAGAAVAAEEPILNVYNWNDYIAADTIPAFE
                     KASGIEVRYDLYDSNATLQGKLLTGGSGYDVVYPSVEYAGKQIQAGIFQPLDKSRLPN
                     LVHIDPLILEAVAVADPGNRYLVPYMWFTIGVAINVDKVMKALDGKLPDNAWDLLFDP
                     ALTARLKGCGIALMDEASDVIPAAMLDAGLDPVKMDPADIRAAVEHIRPVRKDIRSFN
                     TAPIEQMAKGSLCVAMMFSGDARIAARRAQIGGSEVRLQYLIPAVGAMMSVDVMAIPR
                     DAPHPHNAHRWIDAMMDPATVARISNETFYISANTAALALTDATLRDDPAINVPEAVK
                     RTLRAKPVLGKDVQRELTQALNRFKAAR"
     gene            complement(7374..8681)
                     /gene="hisD"
                     /locus_tag="PKV97_01465"
     CDS             complement(7374..8681)
                     /gene="hisD"
                     /locus_tag="PKV97_01465"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNB04569.1"
                     /translation="MSQTPIRRLAAREPEFLSTLDALLAFEAEADGRIDAAVTEILQA
                     VRTTGDAAVVEYTRRFDGLDVQSMVALELPRSELLLALDSLRPEQREALTIAADRVRV
                     YHERQKGESWEFTEADGTRLGQKVTPLDRVGLYVPGGRASYPSSVLMNAIPAKVAGVG
                     ELIMVVPTPRGEKNPLVLAAAAITGVDRVFTIGGAQAVAALAYGTQTIPQVDKIVGPG
                     NAYVAEAKRRVFGTVGIDMVAGPSEVLIISDGSGHADWVAMDLFAQAEHDELAQSILL
                     CTDAGFIDAVHDAIDRLLPTMPRRDTIARSLANRGALIHVDSLEQACALANRIAPEHL
                     ELALEDAEDWIAHIRHAGAIFVGHWAVEALGDYCAGPNHVLPTMRSARFSSPLGTYDF
                     QKRTSIVHISQAGAQHLGKVASILAHGEGLQAHARSAEMRLKV"
     gene            complement(8695..9339)
                     /gene="hisG"
                     /locus_tag="PKV97_01470"
     CDS             complement(8695..9339)
                     /gene="hisG"
                     /locus_tag="PKV97_01470"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNB04570.1"
                     /translation="MSSITLALSKGRIFEETLPLLAAAGIVPTDNPESSRKLIIGTNR
                     PDVRLVIVRATDTPTYVQYGAADLGIAGKDVLVEHGGAGLYQPLDLEIAKCRLCVAVQ
                     KGFDYAAATRPGGRIRVATKYMNAAKAHFAGKGMHVDLIKLYGSMELAPLVGLADAIV
                     DLVSTGGTLRANNLEEVEDIMPISSRLIVNQASLKLKRELLQPVLDAFAGAIQS"
     gene            9492..9899
                     /gene="rnk"
                     /locus_tag="PKV97_01475"
     CDS             9492..9899
                     /gene="rnk"
                     /locus_tag="PKV97_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNB04571.1"
                     /translation="MKPSIIVSSSDLERLEGLLTLPAFRSRSDLDGLRAELERADVRE
                     PQDMPADVITMNSRARFVEEGTGREYELTLAYPKDANAEAHRVSIFSPAGSALLGLSA
                     GQSIDWKSADGKEIRLKVLEVTWQPEANGQFEL"
     gene            complement(10017..11267)
                     /gene="murA"
                     /locus_tag="PKV97_01480"
     CDS             complement(10017..11267)
                     /gene="murA"
                     /locus_tag="PKV97_01480"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNB04572.1"
                     /translation="MDRLLIEGGARLAGEIAISGAKNAALPILCAALLTAEPVTFTNV
                     PRLNDIDTLLALLAQMGVRVKREADVVMLDASALDNPVAPYEMVKTMRASILVLGPLV
                     ARCGEARVSLPGGCAIGARPVDQHIKGLQAMGAAVGVEHGYVHAAVPRLRGARLFTDM
                     VTVTGTENLMMAAALADGETVIENAAREPEVVDLANCLVAMGAQISGAGSDVIRIRGV
                     ERLHGATHRIMPDRIETGTYLCAAAVTGGSVRLTGTSSCYLDAVIDKLMDAGCEVVSE
                     RDAIRLTAPARLNAVSLRTAPYPAFPTDMQAQFMALNAVAKGVAMIRETIFENRFMHA
                     VELQRLGADIRIDGNTAVVQGVDRLQGATVMATDLRASASLVVAGLVAEGDTTIERIY
                     HLDRGYERLEEKLAALGARVRRLG"
     gene            complement(11275..11532)
                     /locus_tag="PKV97_01485"
     CDS             complement(11275..11532)
                     /locus_tag="PKV97_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HNB04573.1"
                     /translation="MFEASEVKRLIEQGLPCELVVIEGEDGVHFRGIVVSAAFEGKMK
                     VRQHQAVYATLGRLMGNEIHALQLQTFTPAQWEEGRGELGM"
     gene            complement(11543..12316)
                     /locus_tag="PKV97_01490"
     CDS             complement(11543..12316)
                     /locus_tag="PKV97_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB04574.1"
                     /translation="MAAESPLAGFRTLLYKEVLRFWKVSFQTVAAPVLNALLFLLIFS
                     HVLDRHVTVYGEVAYTSFLVPGLVMMSVLQNAFANSSSSLIQSKITGNIIFVLLPPLS
                     YREFYAAYVIASILRGLMVGAGVLAVSAAFVVVPMAHPLWVLGFAVLGGAILGSLGVI
                     AGIWADKFDQLAAFQNFLIMPLTMLSGVFYSIHSLPRFWQDVSHFNPFFFMIDGFRYG
                     FFGQSDTSPWLSLGVVGLCFVLLAALTLAMLARGYKLRS"
     gene            complement(12351..13247)
                     /locus_tag="PKV97_01495"
     CDS             complement(12351..13247)
                     /locus_tag="PKV97_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB04575.1"
                     /translation="MSTPAIRIQGVSKHYGSLKALGGVDLEVGQGEFFGLLGPNGAGK
                     TTLISALSGLVRPDSGTLAIMGHDVVADYRNARRNLGVVPQELVFDPFFSVRELLRIQ
                     SGYFGIRSNDDWIDEILASLDLTHKASANMRMLSGGMKRRVLVAQALVHRPPVIVLDE
                     PTAGVDVELRQGLWQFIRKLNRDGHTIVLTTHYLEEAEALCGRIAMLKAGRIVALDTT
                     ANLLRRFATHSLSVRLEHPARGVELGGSAGEGGWVDFAFDSYGEVETLLARLREGGAG
                     LVEMRLGEPDLERVFVEVMNRA"
     gene            complement(13244..13528)
                     /locus_tag="PKV97_01500"
     CDS             complement(13244..13528)
                     /locus_tag="PKV97_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB04576.1"
                     /translation="MSDPALFAPAGALTMQSAPALLAEGQRRARAGDLVVDLAAVSAA
                     DSAALALILDWMRCARAAGHALMLRNPPAGLTSLAALYDIDGLLPLERAA"
     gene            complement(13525..14187)
                     /locus_tag="PKV97_01505"
     CDS             complement(13525..14187)
                     /locus_tag="PKV97_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB04577.1"
                     /translation="MNKILDFLRAFLLCALLGAALPATASADKAPDVLVRDVTTEVLE
                     IVRADKAIQAGDTQRVIALVDAKVLPHFDFRRMTMLAVGRDWRQASAEQQTRLTDAFR
                     TLLVRTYSNALTQYRDQRIEFRPSRFAPADTRVQVRTEVVQAGAQPIGIDYMLEKGEQ
                     GWKVFDVIVAGVSLVTNYRSSFGQEIARGGIDGLIRSLEERNRALAEQQGMSQGDAGR
                     GA"
     gene            complement(14197..15048)
                     /locus_tag="PKV97_01510"
     CDS             complement(14197..15048)
                     /locus_tag="PKV97_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNB04578.1"
                     /translation="MRNVFRSSRAGLVAAALSAALATGCATTANPDDPLEGYNRAMFG
                     FNEQVDKVVIKPAAQVYEAVLPHPVRTGVGNVLGNLGDPWVALNNLLQGKVAEGMGDL
                     MRFALNSTWGLLGLLDVAGEAGLPKHDEDLGQTLGRWGVGEGAYIVLPFFGPRTVRDA
                     VALPADFIANEPFSIDHVATRNTVLGTRVVHLRSTLLGADRTLEEAALDRYVFVRDFY
                     LEQRRYKVSDGRQERVYEDFDEQSAAPPGGDVDTAAASAVGNLEHADIGASPATAGRR
                     VARQSVQ"
     gene            complement(15078..15545)
                     /gene="mlaD"
                     /locus_tag="PKV97_01515"
     CDS             complement(15078..15545)
                     /gene="mlaD"
                     /locus_tag="PKV97_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNB04579.1"
                     /translation="MSRTALDLWVGLFVAIGFAALVFLAMKVGNFSGLDGAETYRVEA
                     PFDNIGGLKVRAPVKSSGVLVGRVAVIRFDPAIYRAVVELEIDKRYAFPADTTASILT
                     SGLLGEQYVGLEAGADTAMLKGGDRVLITQSAVVLEKLIGQFMFDRAADAPAQ"
     gene            complement(15548..16336)
                     /gene="mlaE"
                     /locus_tag="PKV97_01520"
     CDS             complement(15548..16336)
                     /gene="mlaE"
                     /locus_tag="PKV97_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HNB04580.1"
                     /translation="MSAIASALRRIGALTIDGIWRLGFATRFLFAVLLNSGQSLRRIH
                     LTIRELYFSGVLSLLIIMVSGLFVGLVLGLQGYDILQRYGSADAVGVMVALSLLRELG
                     PVIAALLFASRAGSAITAEIGLMKTTEQLKAMDMMAVNPIARVVAPRFWGGVLSMPLL
                     AALFSAMGVFGGWVITVVVIGVDDGAFWSQMQAAVEFRYDVVNGVIKSAVFGTVVSLI
                     AVFEGYDCQPTAEGVSRAITRTVVTSALAILALDFVLTSFMFRG"
     gene            complement(16333..17154)
                     /locus_tag="PKV97_01525"
     CDS             complement(16333..17154)
                     /locus_tag="PKV97_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB04581.1"
                     /translation="MTVSAPHPSPLVSLRDVRFAYGEREVLRGIDLSVHRGQVVAIMG
                     GSGCGKTTLLRLIGGQLRATGGTVEVEGRDVARLSGSELYALRRRMGMLFQFGALFTD
                     MSVFDNVAFPLREHTDLPAGMVRDLVLMKLQAVGLRGAAKLHPNELSGGMARRVALAR
                     AVALDPLLILYDEPFAGLDPISLGIIGQLIRKLNDALGASSVMVTHDVHESLAIVDYI
                     YFVSDGRIVAQGTPDEIRASRDPFVHQFVHAEADGPVPFHYPAEPIERLLGGEGA"
     gene            17352..19229
                     /locus_tag="PKV97_01530"
     CDS             17352..19229
                     /locus_tag="PKV97_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNB04582.1"
                     /translation="MTFTLTDLRRLLATAALLATAACAPLGRPPALEGHLDKRTAIER
                     TAPVGTATRSLAPPIAESPSSSRGAAASAATGAPPPAPARGRAAPAVRGYSIDVFDIP
                     AEQLLLAIGRDAGLELDLHAGITGNVSLQAHDRPLGELLERIARQVDLRYELRGRHLR
                     VRPDTPYIRSYAVDYVNLSRDMRGTVATSTQIATSGSAGGAGQRASSTGGAGNASITQ
                     IETRSANDFWNTLEHNLAAILAGPGRAPDCGGERRGESPAGCRNADLMLNRESGIVMA
                     RATQRQHEQLEAFLAGVQRAARRQVMIEATIVEVTLADGYRQGIDWTRLGVPSWSVRP
                     RGSGDATPGQPTLSYLSANSDIRLELLETFGTVKVLSSPRLSVLNNQTAMLKVVEEVV
                     YFLVDSTTTEYDDRKQARISATTTPQSVSVGMVMSVTPQISADGDITLNVRPTISGIS
                     GFKDDPNPSLGDIPNRVPQIRTREIESMLRLRSGEVAVLGGLMEDRIDHDTGRIPLFG
                     DLPVVGELFTRRDNNVRRTELLIFLRPLLIDEPGMRGGYAEYAGHLPDDDFLLDSPSP
                     GQRNFPHLPLRPLLAPAGAVSAAEGFSEAAYPDPADPRSPAASSTSASAANADAGTSP
                     P"
     gene            19226..20941
                     /locus_tag="PKV97_01535"
     CDS             19226..20941
                     /locus_tag="PKV97_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNB04583.1"
                     /translation="MNAPLPIGQILIAAGLIGEDQLRIALHEQRGRARPLGRVLVELG
                     FVSEAALREALAARSGLPCVDLASALADPDAIARVPQALARRHRLLPLQYDAARHRLI
                     VAMADAHDIVALDRLRAELGPDVHVELRLAGDNELGRAIEQHYGQASSIEDMVRELER
                     RAGQPIAGARDPQLVVRLVDALLAEAAARGASDLHLEPEAGFLRVRHRIDGTLRQVRA
                     MHKSCWAELAVRIKVLAGMDIAESRSPQDGRIGLALGGRPIDFRVATQPTLHGENIVL
                     RILDRDKGIVPLDALGLDPAQRAALDRILARPEGLILVVGPTGSGKTTTLYSMLGHLN
                     TEAVNIMTLEDPVEYPLALIRQTQVGDASRLDFADGVRALLRQDPDVILIGEIRDADT
                     ATMALRAALTGHQVFATLHANSIFGALPRLHEIGIGAELLAGNLCGILAQRLVRRLCP
                     TCREPVPDDAPERHLPGLAADAPPAPSWRARGCPECDFRGYRGRLAIMEILPFDAELD
                     ELVARRASPGELRAAARTRGHRSLAEDGLRRVLDGSTSLAELARVVDLSALAATAKTA
                     HGGEA"
     gene            21021..22223
                     /locus_tag="PKV97_01540"
     CDS             21021..22223
                     /locus_tag="PKV97_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNB04584.1"
                     /translation="MSTWRYRALAADGRRVRGELDAADLGELERLLEARALLLIDAQP
                     RGVRGLLPRRRIARRELIPFCYHLEQLLAAGVPPFESLALLRDAGAEPRLQQVIGALI
                     ADVERGLPLSGAAARQPEAFSAVAVSLLRAGEQAGRLPEAIRDIGAALAQEEELASHA
                     RRIAIYPAIVATVLLAAVVVALIHVVPELERLLRGTGQRLPLQTRILVGLSAAVRDWG
                     WAMLLVTLAGAASSAHALARSEALRTRVHALLLQLPLVGGILHKLALARFAALFATLY
                     GAGINVIDALHTSTGATANLALRAGLREATGAIEQGRPISVAFEATRVFPPLVIRMLR
                     LGEHTGALDDALAKVASLYRRDVAEGIARLQAAAEPALTILMGGLLLWIASAVLGPIY
                     ELITRLPV"
     gene            22226..23728
                     /locus_tag="PKV97_01545"
     CDS             22226..23728
                     /locus_tag="PKV97_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04585.1"
                     /translation="MRREPLLLAVAPSGLLACIGHGDGITLLAAFTCGEEAAFASWLA
                     RRPPDEPCRMLVDLPDESYQIEDLPRVRGSDRRALFARRLAHWFPEPRFARATPLGAL
                     PDGRQGAERVLFAGMESSTDLLPWLDRLAADGRRPQILVPASALLPRLPLPGARQRRH
                     GKAPPRPRLLATHGRAGLRISLLAGEHTLFSRLVRGHADSLADPQALATELERTRDYL
                     AAQHRIASDAPLDRIVADLPAQTGTEHATLPPIGVAAADTLAGCGLPNSYDAHLLLAL
                     RHAPTTIGWPLAAGARRWPRLPERRVLGALALVASTILGALVWMEHQAQVEAAALAAT
                     ERARAARAAALAAEEAELAAREAERAALAALDAAPPPLPTPAPAPAEPAPAACPPASS
                     PPPGPIARRIDGVLRRPDGEILLWVEGTWQSARALGLHPIAGDAAVVVAAGRRTQLRS
                     GELVPTAVATVTAGPGQAYPERDDAAPADRAAGPADSAARPSVDMRGARP"
BASE COUNT         3692 a         8616 c         8090 g         3392 t
ORIGIN      
        1 cttggtgccg tcgcggtcca tgctggtgag caggatctcg cccgccccca gcgactcgac
       61 cttgcgcgcc cactcgatgg cgtccagccc ggtgttgttg cgcccgccgt gggtgaacac
      121 ctcccacttg ccgggcgcgg tctgcttggc gtcgatcgcc accacgatgc actggctgcc
      181 gaccttgctc gcggcctcga agaccagctg cgggttgttc accgccgcgg tgttcatgct
      241 gaccttgtcg gcgccggcgt tgagcagccg gcgcacgtcc tcgaccacgc gcacaccacc
      301 gccgacggtg agcgggatga agacctgctc ggcgacctgc tcgaccacgt gcaggatgat
      361 gtcgcggtcg tcggagctcg cggtgatgtc gaggaaggtg atctcgtcgg cgccctgctc
      421 gtcgtagcgg cgggcgatct cgaccggatc gccggcgtcg cgcaactcga cgaaattgac
      481 ccccttgacc acgcgcccgg acttcacgtc caggcaggga atgatgcgct tggctagcat
      541 cggctgcccc tgcgggagaa gctcattcgc gcgcgccctc gagctcgtcc gcgcgcgcct
      601 gcgcggcggc gaagtcgagc gtgccctcgt agatcgcgcg cccggtgatc gcgcccatga
      661 tgccctcgtc ctcgaccgcg cacagcgcgt cgatatcgcg caggtcggtg atgccgccgc
      721 tggcgatgac cgggatgcgc agcgcgcgcg ccagacgcac ggtggcctcg atattgacgc
      781 ccgaaagcat gccgtcgcgg ccgatgtcgg tgtagatcac cgactccacg ccatagtcct
      841 cgaacttcct cgccagatcg accacgtcgt ggccggtgag cttggaccat ccgtccaccg
      901 ccaccttgcc gtccttggcg tcgaggccga cgatgatgtg gccggggaag gcgctgcagg
      961 cgtcgtgcag gaagccgggg ttcttgaccg cggcggtgcc gatgatgacg tagctgatgc
     1021 cgttgtcgag gtaatgctcg atcgtgtcga ggtcgcggat gccaccgccg agctggaccg
     1081 ggatgtcgtc accgacgacg tcggtgatcg aacggatcgc ggcgccgttc ttcggcttgc
     1141 cggcaaaggc accgttgagg tccaccaggt gcaggcggcg cgcgccggcc tcgagccagt
     1201 ggcgcgccat ggcgcccgga tcggaggaga acaccgtggc gtcgtccatc tcgccctgtt
     1261 tcaggcgaac acattgaccg tccttgaggt cgatggcggg aatgagcagc ataccgggat
     1321 cagcagtgga gaaacgtaga gggaatcacc ggccgctcag ggctgccagc ggatgaaatt
     1381 ggcgagcagg cgcaggccgg ccgcggcgct cttctcgggg tggaactgag ccgcgaagat
     1441 attagcccgc gctaccgcac tggtaaagcg aacgccatag tcggtctcgg ccgcagtcag
     1501 cgaggcctcc gcaggcacca cgaagtagct gtgcacgaag tagaagcgct cgccgtcggg
     1561 gatgccgtcc cacatcgggt gcggtccgca ctgacgtact tcgttccagc ccatgtgtgg
     1621 caccttgagc cggctgccgt cggccgcgaa catgccggtc tcgggaaagc gcacgacctc
     1681 cccggacagg atgcccaagc ccggcacgtc gccctcggcg ctgtgctgga acagcatctg
     1741 ctggccgatg cagatgccga ggaagggttt ttcggccgcc gcggcgcgta ccgcaggtcg
     1801 caggccgcgc gcgtcgagtt cgcgcatgca gtcgggcatc gcgccctgcc ccggaaacac
     1861 gacccgctcg gcggcggcga cacgcgccgg gtcggaggtg acgaagacct catgaccggg
     1921 cgccacgtgt tcgatcgcct tggcgaccga gcgcaggttg cccatgccgt aatcgatgat
     1981 ggccacgctg gtcatcggct tgactcgctt ttcgacagga tggcggccgc gcgacgcgac
     2041 cgcgggaagg gaggaatcag agcgcgccct tggtcgaggg gatggtcccg gcggcgcgct
     2101 cgtcgtgctc ggccgccatg cgcagcgcgc gggcgaaggc cttgaacacg gtctcgcact
     2161 gatggtgggc gttgtcgccg cgcaggttgt cgatgtgcac cgtgacgccg gcgtgattga
     2221 cgaagccctg gaagaactcg cgcgccagat ccacgtcgaa gttgccgatg cgggcgcggg
     2281 tgtaattcac gaagtagtgc agccccgggc ggccggagaa atcgaccacc acgcgcgaca
     2341 gcgcctcgtc gagcggcacg taggcatggc cgtagcggcg cagccccttc ttgtcgccga
     2401 gcgccttggc gaaggcctgc ccgagcgtga tgccgacgtc ctctacggtg tggtggtcgt
     2461 cgatgtgggt atcgccctcg cagtggatgt cgaggtcgaa caggccgtgg cggacgatct
     2521 ggtcgagcat gtggtcgaag aagggcacgc cggtgtcgag ccggccctgc ccgctgccat
     2581 cgaggtcgat gcgcacggtg atcttggttt cgagggtgtt gcgcgtgact tctgcttgcc
     2641 gcatgggagc taccggccaa aatgggttgt ctgcccgtca tgataacatc gcgcagcgaa
     2701 ctcgccgcga tgcgccgcaa ggtgcgttgt gcgcgtccaa gcctccgttc caccccaacc
     2761 gcagacaccc gcatgagccg cttctggagc gccgtcgtcc acggcctgac cccctacgtg
     2821 ccgggcgagc agcccaagct cgacaacctc gtcaagctca acaccaacga acacccctgc
     2881 ggcccctcgc cgaaggtgct ggaggccatc cgcgccgcca ccggcgacgg cctgcgcctc
     2941 taccccgacc ccaacgccga cgcgctcaag gccgcgctcg cccgccatca cggcgtgcgc
     3001 ccggagcaga tcttcgtcgg caacggctcc gacgaggtgc tcgcgcacgc cttcatggcg
     3061 ctgctcaagc acgagcgcgc gctgtggttc ccggacatca cctacagctt ctatccggtg
     3121 tattgcgggc tgtacgacat ccagccgcgc atcgtgccgc tggccgagga cttctcgatc
     3181 cgcgtggaag attacctgcc gcgcggcgac gagcgcgccg gcgcgatcat cttccccaac
     3241 ccgaacgcac ccaccggacg gctgctcgcg ctcgccgagg tcgagcgcat cgtcgccgcc
     3301 aaccccgagg ccgtggtgct ggtcgacgag gcctacgtgg actttggcgg cgacagcgcg
     3361 atcgcgctgg tggatcgcca ccccaacctg ctggtcacgc gcaccttctc caagagccgc
     3421 gcgctggcgg gcctgcgcgt gggctacgcg gtgggcgacg cggcgctgat cgaggcgctg
     3481 gagcgggtca agaacagctt caactcctac ccgctcgacc gcctcgcgat cgccggcgcg
     3541 gtggcctcgg tggaggacga ggactacttc cgcgctagct gcgcccaggt gatcgagacg
     3601 cgcgcgcggc tggtcgccga cctgcaggcg ctcggcttcg aggtgctgcc ctcggcggcg
     3661 aacttcatct tcgcccgcca cccgacgcgc gacggcgccg aactcaccgc cgaactgcgc
     3721 aggcgcgcga tcatcgtgcg ccacttcaag gcggcgcgca tcgaccagtt catgcgcatc
     3781 accgtcggca ccgacgcgca gtgcgcgatt ctggtggcgg ccctgcgcga gatcctcgcc
     3841 ggctgaagcc gatcagccgc cgcgcgccac cgctggcgcc gacgcnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnccggg tggtgatctc cccggcggtg aaggagaaca
     4021 cgatcaggca caggatgggc acgtagagga aggcgtacac gccgaccacc cacatccgtc
     4081 cgccccagcc gccgccgttc attcgtccct cccggccccg cgcccatggc gctgcagcag
     4141 cacgatcggc gcgatcagca tcgccaccat gagcaccgcg accgccgccg ccatcggcca
     4201 gtcctggttg gggccgaagt cgtcccagat gctgcgcccg atcgtcatca cgctgccgcc
     4261 gccgagcaga tcggggatga cgaactcacc caccgccggg atgaacacca gcatcgagcc
     4321 ggcgacgatg ccgcgcgagg acagcggcag ggtgatgcgc aggaacaccg tggccggccg
     4381 cgcgccgagg tcgcgcgcgg cctcgggcag gcgctggtcg agcttgacca ggttggcgta
     4441 gagcggcagc accatgaagg gcaggtagct gtagaccatg ccgaccagca ccgcgccggt
     4501 gttgtagagc agcggcagcg gctccgccac caggccgagg ccgagcagca cctggttgat
     4561 cagcccggtc tcgctgctgt cgagcaggcc catccaggca tagacgcgca gcaggaagga
     4621 ggtccagaac ggcagcatca ccagcatcag caggaggttg cgcatcgcct cgggcgcacg
     4681 cgcgaggcgg taggcgaagg gatagccgag aaccagacag cacagcgtgg tgagcgtcgc
     4741 gagcaggagc gaattggcgt aggcggcgag atagggccgg tccgcggcga gcgtgccgag
     4801 tgccgccagc tcgccgccga ggcgggcgta gctggcggga tcgggacgga aggcaggcgc
     4861 cccgtccgca gccgcgccgg cgaagggcga cacgtagggc ggcacggcga ggtccggcgt
     4921 ggacaggctg acgttcacga tcagcgcgaa gggcagcacg aagaagaaca gcatccacac
     4981 ccacggcagc aggccgaccc agaagcggcc atgcccttgc gcagaagccg gacgcgcctt
     5041 catcgcagca gcagcacgcc gtcgggcgcc ggccacgaca cccgcaccgg ctcgccgcgc
     5101 gccggcggcg cggcctcgcc ccagtgcgcc gcgggcacgc tggcgatcat cgagccaccg
     5161 ccgggcaggc gcaggtggta gatggagcgg ccgccgagat aggcgatctc ggcgacctcg
     5221 gccggcgcgg cgttgtcatc gggctgttcc ggcacgcacg catcgccggc ggcgcgatga
     5281 tgcacgcgca cgcactcggg gcgcagcgac agccaggctt cacccccctg ctgcagcgca
     5341 acggtggcca cgaagcgaat cggctgcgga aactcgccgc cgaccaggcg atcgccctcg
     5401 atgcggccgt ggaagaggtt ggtctcaccg atgaagctcg cgctgaagcg gcagttgggg
     5461 cgctcgtaga tctcacccgg cgtgccgacc tgcaccagcc cgccctcgga caggatgccg
     5521 atgcggtcgg ccatggtcat cgcctcctcc tggtcgtggg tgacgaggat gcacgtgacc
     5581 cccacacgcc ggatcaggcc gacgagctcg aactgggtct gctggcggat gcgcttgtcg
     5641 agcgcggcga gcggctcgtc gagcagcagc aggcgcggct cgcgcgccag gctgcgcgcc
     5701 agcgccacgc gctgctgctg tccgccggag agcgcgtgtg ggcggcgccc ggcgtaggcg
     5761 cgcatctgca ccagctcgag catctcgtcg acgcgggccg cgatcgccgc gcgcgccatg
     5821 cgcggccgca actggcgcag gccgaaggcg acgttgcccg ccaccgtcat gtgcgggaac
     5881 agcgcgtagg actggaacat catgttggtc ggacgacggt gcgcgggcac cgcggcgaga
     5941 tcctcgccgt cgagcaggat gcgcccgcaa ctcggcgtct ccaggccggc gatcatgcgc
     6001 agcaaggtgg acttgccgca gcccgagctg cccagcagcg cgaagatctc gcgctcgccg
     6061 atgtcgagcg agacctcgcg caccgcccag gtgtcgccga agcgttttcc caggctttcg
     6121 atgcgcagat gaacgcgctc gccggccaag ctttcgggcc catcctgcgg catgcgctcg
     6181 ctcattgcat cacccgctgc gcccgcccgc cgctcagcgt gccgccttga agcggttcag
     6241 cgcctgggtc agctcgcgct gcacgtcctt gccgagcacc ggcttggcgc gcagcgtacg
     6301 cttgaccgcc tccggcacgt tgatcgccgg gtcgtcgcgc agcgtggcgt cggtgagcgc
     6361 gagcgccgcg gtgttggcac tgatgtagaa ggtctcgttg gagatgcgcg ccaccgtggc
     6421 cgggtccatc atcgcgtcga tccagcggtg ggcgttatgc gggtgcggcg cgtcgcgcgg
     6481 gatcgccatc acgtccaccg acatcatcgc gcccacggcc gggatcaggt actgcagcct
     6541 gacctctgag ccgccgatct gcgcgcgccg cgcggcgatg cgggcgtcgc ccgagaacat
     6601 catcgccacg cacaggctgc ccttggccat ctgctcgatg ggcgcggtgt tgaagctgcg
     6661 gatgtccttg cgcaccgggc ggatgtgctc gaccgcggcg cggatgtcgg ccgggtccat
     6721 cttcaccggg tcgaggccgg cgtcgagcat ggccgcgggg atcacgtcgc tggcctcgtc
     6781 catcagcgcg atgccgcagc ccttcaggcg cgcggtcagc gcagggtcga agagcaggtc
     6841 ccaggcgttg tcgggcagct tgccgtcgag cgccttcatg accttgtcga cgttgatcgc
     6901 caccccgatg gtgaaccaca tgtagggtac gaggtagcgg ttgccggggt cggcgaccgc
     6961 caccgcctcg aggatcagcg ggtcgatatg gacgagattg ggcaggcgcg acttgtcgag
     7021 tggctggaag atgccggcct ggatctgctt gccggcgtat tccacgctcg ggtacacaac
     7081 gtcgtagccg ctgccgccgg tcagcagctt gccctgcagc gtggcgttgc tgtcgtagag
     7141 gtcgtagcgc acctcgatcc cgctcgcctt ctcgaaggcg gggatggtgt cggcggcgat
     7201 gtagtcgttc cagttgtaga cgttgaggat gggctcctcg gccgccaccg ccgccccggc
     7261 ggccagggcc agcaccgcca tccagccgaa cttcagcgtc atgcccgcct ccccgcgatc
     7321 gacaagggaa caccgcctgc ccgcccgggt cgggggaccc gggcgggcag gcgtcaaacc
     7381 ttcagccgca tctccgccga gcgggcgtgc gcctgcagac cctcgccatg ggccaggatg
     7441 gaagccacct tgcccaggtg ctgcgcgccc gcctgcgaga tgtggacgat gctggtgcgc
     7501 ttctggaagt cgtaggtgcc gagcggcgag gagaaacgcg cgctgcgcat ggtcggcagc
     7561 acgtggttgg ggccggcgca gtagtcgccg agcgcctcca ccgcccagtg gccgacgaag
     7621 atcgcgccag cgtgacggat gtgggcgatc cagtcctcgg catcttccag cgccagctcg
     7681 aggtgctcgg gcgcgatgcg gttggcgagc gcgcaggcct gctccaggct gtcgacgtgg
     7741 atcagcgcgc cgcggttggc aagcgacctg gcgatcgtgt cgcggcgcgg catggtgggc
     7801 agcaggcggt cgatcgcgtc gtgcaccgcg tcaatgaagc cggcgtcggt acacagcagg
     7861 atggactgcg cgagctcgtc gtgctcggcc tgggcgaaga ggtccattgc cacccagtcg
     7921 gcgtggccgg aaccatcgga gatgatcagc acttcagacg ggccggcgac catgtcgatg
     7981 ccgacggtgc cgaacacgcg gcgcttggcc tcggccacgt aggcattgcc cgggccgacg
     8041 atcttgtcca cctgcgggat ggtctgcgtg ccgtaggcca gcgccgccac cgcctgcgcg
     8101 ccgccgatgg tgaacacgcg gtcgacgccg gtgatcgccg ccgccgccag caccagcgga
     8161 ttcttctcgc cacgcggggt gggcacgacc atgatcagtt cgccgacacc ggcgaccttg
     8221 gccgggatcg cattcatcag caccgagctc gggtaggagg cgcgcccgcc cggcacgtag
     8281 aggccgacgc ggtcgagcgg ggtgaccttc tggcccaggc gggtgccgtc ggcctcggtg
     8341 aattcccagg actcgccctt ctggcgctcg tgatagacgc gcacgcggtc ggcggcgatg
     8401 gtgagcgcct cgcgctgctc ggggcgcagg ctgtcgagcg cgagcagcag ctcgctcctg
     8461 ggcagctcga gcgcgaccat ggattgcacg tcgagcccgt cgaagcggcg ggtgtattcg
     8521 accaccgcgg cgtcgccggt ggtgcgcacc gcctgcagga tctcggtgac cgccgcgtcg
     8581 atgcggccgt cggcctcggc ctcgaaggcg agcagggcat cgagggtgga caggaattcg
     8641 ggttcgcgcg cggccaggcg gcggatcggg gtctggctca tcggtaggga ctcctcagga
     8701 ctggatcgcg ccggcgaagg cgtcgagcac cggctgcagc agttcgcgct tgagcttgag
     8761 cgaggcctgg ttgacgatca ggcgcgagct gatcggcatg atgtcctcga cctcttcgag
     8821 gttgttggcg cgcagcgtgc cgccggtcga caccaggtcg acgatggcgt cggcgagccc
     8881 gaccaggggt gcgagctcca tcgagccgta gagcttgatc aggtccacat gcatgccctt
     8941 gccggcgaag tgggccttgg ccgcgttcat gtacttggtg gcgacgcgga tgcgcccgcc
     9001 cgggcgggtc gcggcggcgt agtcgaagcc cttctgcacc gccacgcaca gccggcactt
     9061 ggcgatctcg aggtcgagcg gctggtagag acccgcaccg ccgtgctcga cgagcacgtc
     9121 cttgccggcg atgccgaggt cggcggcgcc gtactggacg taggtgggcg tatcggtggc
     9181 gcgcacgatg accaggcgca catccggccg gttggtgccg atgatcagct tgcgcgagga
     9241 ctcggggttg tcggtgggca cgatgcccgc tgcggcgagc aacggcaggg tctcttcgaa
     9301 gatgcggccc ttggacaggg cgagcgtgat gctggacacg tgaaaagcct tgatcggggg
     9361 gactcgtcat tgtaacccgg cgccgcttct gttaaccttc gacgcgactg gctgaaccgc
     9421 acgcggcggt tcggcctttt cttttgtttc gccgcagcgt cggcgcgcac ctcaggcagg
     9481 aaacccgcac catgaaaccg tcgatcatcg tctccagcag cgacctcgag cgcctcgagg
     9541 gtctgctcac cctccccgcc ttccgcagcc gcagcgacct cgacggcctg cgcgcggaac
     9601 tcgagcgtgc cgacgtgcgc gagccgcagg acatgccggc cgacgtcatc acgatgaaca
     9661 gccgcgcgcg cttcgtcgag gaaggtaccg gccgcgaata cgagctgacc ctggcctacc
     9721 ccaaggacgc caacgccgag gcgcaccgcg tgtcgatctt ctcgcccgcg ggcagcgccc
     9781 tgctcggtct ttcggccggc cagtcgatcg actggaagtc ggccgacggc aaggaaatcc
     9841 gcctgaaggt actggaagtc acctggcaac ctgaagccaa cggacagttc gaactctaag
     9901 cgctcccgca gcgttccgcg gctgccctgc gggagcgacg caagtcgcag ttcggcgacg
     9961 gcacgacggc cgcgtgcacg cgagcgcatg ccgccgtcgc agctctcaaa ctcctctcaa
    10021 ccgagccgcc gcaccctcgc cccgagcgcg gcgagcttct cctccagacg ctcgtagccg
    10081 cgatcgaggt ggtagatgcg ctcgatcgtg gtgtcgccct ccgccaccag gccggctacg
    10141 accaggctcg ccgacgcgcg caggtcggtc gccatcacgg tcgcgccctg cagcctgtcc
    10201 acgccctgca ccaccgcggt gttgccgtcg atgcggatgt cggcgccgag gcgctgcaac
    10261 tcgaccgcgt gcatgaagcg gttctcgaag atggtctcgc ggatcatcgc cacgcccttg
    10321 gcgaccgcat tgagcgccat gaactgcgcc tgcatgtcgg tcgggaaggc cggataaggc
    10381 gcggtgcgca ggctcacggc attgagccgt gccggcgcgg tcaggcggat cgcgtcgcgc
    10441 tccgacacca cctcgcagcc ggcgtccatg agcttgtcga tcaccgcgtc gaggtagcag
    10501 gacgaggtgc cggtcaggcg caccgaacca ccggtgaccg ccgccgcgca caggtaggta
    10561 ccggtctcga tgcgatcggg catgatgcgg tgggtcgcgc cgtggagccg ctcgacgccg
    10621 cggatgcgga tcacgtcgct gccggcgccc gagatctgcg cacccatcgc caccaggcag
    10681 ttggcgagat cgaccacctc gggctcgcgc gcggcgttct cgatcacggt ttcaccatcg
    10741 gccagcgccg cggccatcat caggttctcg gtaccggtca ccgtcaccat gtcggtgaac
    10801 aggcgcgcgc ccctcaggcg cggcacggcc gcatgcacat agccgtgctc gacacccacc
    10861 gccgcgccca tggcctgcag gcccttgatg tgctggtcca ccggacgcgc gccgatcgca
    10921 cagccgcctg gcagcgacac ccgcgcctcg ccgcagcgcg cgaccagcgg gccgagcacc
    10981 aggatggagg cgcgcatggt cttgaccatc tcgtaaggtg cgaccgggtt gtcgagcgcg
    11041 gaggcgtcga gcatcaccac gtcggcctcg cgcttgacgc gcacgcccat ctgagcgagc
    11101 agcgccagca gggtatcgat gtcgttgaga cgcggaacgt tggtgaaggt gaccggctcc
    11161 gcggtcagca aggccgcgca caggatgggc agggcggcgt tcttggcgcc ggaaatggcg
    11221 atctcgcccg ccaggcgggc gccgccttcg atcagcagtc tgtccatgcg cggctcacat
    11281 cccgagttcg ccgcggcctt cttcccactg cgcgggcgtg aaggtctgca gctgcagggc
    11341 gtggatctcg ttacccatca ggcggcccag ggtggcatac accgcctggt gctggcgcac
    11401 cttcatcttg ccctcgaagg cagcgctgac gacgatgccg cgaaaatgca caccatcctc
    11461 gccctcgatg accaccagct cgcagggcag gccctgctcg atcaggcgct tgacttcact
    11521 tgcttcgaac atttggaatc ctttacgaac gcaacttgta accgcgcgcg agcatcgcca
    11581 gggtgagcgc cgcgagcagg acgaaacaca ggcccacgac gccgagcgaa agccagggcg
    11641 aggtgtccga ttgaccgaag aaaccgtagc ggaacccgtc gatcatgaag aagaacgggt
    11701 tgaaatgcga cacgtcctgc cagaaccgtg gcagcgagtg gatggagtag aacacccccg
    11761 acagcatcgt cagcggcatg atgaggaagt tctggaaggc ggcgagctgg tcgaacttgt
    11821 ccgcccagat ccccgcgatc accccgagcg aaccgaggat cgcgccgccg agcacggcaa
    11881 agcccagcac ccacagcgga tgcgccatcg gcaccacgac gaaggcggcc gacaccgcga
    11941 gcacgcccgc ccccaccatc aagccccgca ggatcgaggc gatcacgtag gcagcgtaga
    12001 actcgcggta ggacaagggc ggcagcagca cgaagatgat gttgccggtg atcttgctct
    12061 ggatcagcga ggacgagctg ttggcgaaag cgttctgcag caccgacatc atcaccaggc
    12121 cgggcaccag gaagctggtg taggcgacct cgccgtagac cgtgacgtgg cggtcgagca
    12181 catgggagaa gatgagcagg aacagcagcg cgttgaggac cggcgcagcg acggtctgga
    12241 agctgacctt ccagaagcgc agcacttcct tgtagaggag ggtgcggaag ccggcgagcg
    12301 gcgactcggc cgccatcggc tcgatgcgcg ggacggcgtg gggagattgt tcaggcacgg
    12361 ttcatcacct cgacgaagac gcgttcgaga tcgggctcgc cgagccgcat ctcgacgagg
    12421 ccggctccac cctcgcgcag acgggcgagc aaggtctcca cctcgccata gctgtcgaag
    12481 gcgaagtcca cccagccgcc ctcgccggcg ctgccgccca gctcgacgcc gcgcgcgggg
    12541 tgctccaggc gcacggacag gctgtgggtg gcgaagcggc gcagcaggtt ggcggtcgtg
    12601 tcgagcgcga cgatgcgccc ggccttgagc atcgcgatgc gtccgcacaa ggcctcggcc
    12661 tcttccagat aatgcgtggt gagcacgatg gtgtggccgt cgcggttgag cttgcggatg
    12721 aactgccaca ggccctggcg gagctccaca tccacgcccg cggtgggctc gtcgagcacg
    12781 atcaccggcg gccggtggac cagcgcctgt gccaccagca cgcgccgctt catgccgccc
    12841 gagagcatgc gcatgttggc actcgccttg tgggtcaggt cgaggctggc gaggatctcg
    12901 tcgatccagt cgtcgttgct gcggatgccg aagtagcccg actggatgcg cagcagctcg
    12961 cgcaccgaga agaagggatc gaagacgagt tcctgcggca ccacgccgag gttgcggcgg
    13021 gcgttgcgat agtcggcgac gacgtcgtgc cccatgatcg cgagcgtccc gctgtcgggc
    13081 cgcaccagac cggagagtgc cgagatcagc gtggtcttgc ccgcaccgtt ggggccgagc
    13141 aggccgaaga actcgccctg gccgacctcg aggtcgaccc cgccgagcgc cttcaggctg
    13201 ccgtagtgct tgcttacacc ctggatacgg atcgccggcg tgctcatgcg gcgcgctcca
    13261 gcggcagcag gccgtcgata tcgtagagcg cagccaggct ggtcagcccg gcaggcgggt
    13321 tgcgcagcat cagcgcgtgg ccggccgcac gcgcgcagcg catccagtcg aggatcagcg
    13381 cgagcgccgc ggagtccgcg gcggaaaccg ccgcgagatc gaccaccagg tcgcccgcgc
    13441 gcgcgcgccg ctgcccctcg gcgagcagcg ccggcgcgct ctgcatggtc agcgcgcccg
    13501 cgggcgcgaa cagcgcggga tcgctcacgc gccgcgcccc gcgtcgccct ggctcatgcc
    13561 ctgctgctcg gcgagcgcgc ggttgcgctc ctcgagcgag cggatcaggc catcgatgcc
    13621 accgcgcgca atctcctgtc cgaagctgct gcggtagttg gtgaccaggc tgacgccggc
    13681 gacgatcacg tcgaacacct tccagccctg ttcgcccttc tccagcatgt agtcgatgcc
    13741 gatcggttgc gcaccggcct gcaccacctc ggtgcggacc tgcaccctgg tgtctgcagg
    13801 ggcgaagcgc gagggacgga actcgatgcg ctggtcgcgg tactgcgtca gcgcgtttga
    13861 ataggtacgc accagcaggg tgcggaaggc atcggtcagc ctggtctgtt gctcggcgct
    13921 ggcctggcgc cagtcgcgcc cgaccgcgag catggtcatg cggcggaagt cgaagtgcgg
    13981 cagcaccttg gcgtcgacca gcgcgatcac gcgctgggtg tcgccggcct ggatcgcctt
    14041 gtcggcgcgc acgatctcga gcacttcggt ggtgacgtcg cgcacgagca catcgggcgc
    14101 cttgtcggcg ctggccgtcg cgggcagcgc ggcgccgagc aaggcgcaaa gaaggaaggc
    14161 gcggaggaaa tcgaggatct tgttcatggt tctcgcttac tggacggatt ggcgggcgac
    14221 gcggcggccg gccgtggccg ggctcgcacc gatgtccgcg tgttcgaggt tcccgaccgc
    14281 agaggccgcg gccgtatcga cgtcgccacc gggcggtgcg gccgactgtt cgtcgaaatc
    14341 ttcgtacacg cgttcctggc ggccatcgct gaccttgtaa cggcgttgtt cgagatagaa
    14401 gtcgcgcacg aagacatagc ggtcgagcgc cgcctcctcg agcgtgcggt cggcaccgag
    14461 cagcgtggag cggaggtgca caacgcgcgt gcccagcacg gtgttgcgcg tcgccacgtg
    14521 atcgatgctg aaaggctcgt tggcgatgaa gtcggcgggc agcgcgaccg catcccgcac
    14581 ggtccgcgga ccgaagaagg gcagcacgat gtaggcgccc tcgcccacgc cccaacggcc
    14641 gagcgtctgc ccgaggtcct cgtcgtgctt gggcaggccc gcctcgccgg cgacgtcgag
    14701 caggccgagc aggccccagg tggaattgag cgcgaagcgc atcaaatcgc ccatgccctc
    14761 ggcgaccttg ccctgcagca ggttgttgag cgcgacccag ggatcgccga ggttgcctag
    14821 cacgttgccg acaccggtac gcaccggatg cggcagcacc gcctcgtaga cctgggctgc
    14881 gggcttgatc accaccttgt cgacctgttc gttgaagccg aacatcgcgc ggttgtagcc
    14941 ctcgagtgga tcatcgggat tcgcggtggt cgcacagccg gtggcgagcg cggccgacag
    15001 cgcggccgcg accaggcctg cacgggagga gcggaagacg tttcgcattg cagttttctc
    15061 cggagttcct tgccgaccta ctgggcgggc gcgtcggcgg cgcggtcgaa catgaactgg
    15121 ccgatgagtt tctccagcac caccgcggac tgcgtgatca gcacgcggtc gccacccttg
    15181 agcatcgcgg tgtcggcacc ggcctccagc cccacgtact gctcgccgag caggccggag
    15241 gtgaggatgg acgcggtggt gtcggcggga aacgcatagc gcttgtcgat ctcgagctcg
    15301 accaccgcgc ggtagatcgc gggatcgaag cggatcaccg cgacccggcc cacgaggacg
    15361 ccggagctct tgaccggcgc gcgcaccttg agcccgccga tgttgtcgaa gggggcctcg
    15421 acgcgatagg tctcggcgcc gtcgaggccg gagaaattgc cgaccttcat ggcgaggaac
    15481 accagcgcgg caaagccgat cgccacgaag aggccgaccc acaggtcgag cgcggtacga
    15541 ctcatcatca acccctgaac atgaaggagg taagcacgaa atccagcgcc aggatcgcca
    15601 gcgcggaggt caccacggtg cgggtgatcg cgcgcgacac gccctcggcg gtcggctggc
    15661 aatcgtagcc ctcgaacacc gcgatcagcg acaccacggt gccgaacacc gcggacttga
    15721 tcacgccgtt gacgacgtcg tagcggaact cgaccgcggc ctgcatctgc gaccagaacg
    15781 cgccgtcatc gacgccgatc acgaccacgg tgatcaccca gccgccgaac acgcccatcg
    15841 ccgagaacag cgccgccagc agcggcatcg acagcacgcc accccagaag cgcggcgcga
    15901 ccacgcgcgc gatcgggttg accgccatca tgtccatcgc cttcaactgc tcggtggtct
    15961 tcatcaggcc gatctcggcg gtgatcgccg agccggcgcg gctggcgaac agcagcgcgg
    16021 cgatcaccgg accgagctcg cgcagcagcg acagcgccac catcaccccc accgcgtcgg
    16081 ccgagccgta gcgctgcagg atgtcgtagc cctgcagccc gagcacgaga ccgacgaaga
    16141 gccccgagac catgatgatc agcagcgaca gcacgccgct gaaatagagc tcgcggatgg
    16201 tgagatggat gcgccgcagc gactgtccgg agttcagcag caccgcgaac aggaagcggg
    16261 tggcgaagcc caggcgccag atgccatcga tggtcagcgc accgatgcgg cgcagcgcgg
    16321 aggcgatcgc gctcatgcgc cctccccgcc gagcaggcgc tcgatcggct cggccggata
    16381 gtggaagggc accggcccgt cggcctcggc gtggacgaac tgatgcacga agggatccct
    16441 cgaggcgcgg atctcgtccg gcgtgccctg cgccacgatg cggccgtcgg agacgaagta
    16501 gatgtagtcc acgatcgcca gcgactcgtg cacgtcgtgg gtcaccatca ccgagctcgc
    16561 gccgagcgcg tcgttgagct tgcggatgag ctggccgatg atgccgagcg agatcgggtc
    16621 gaggccggcg aagggctcgt cgtacaggat cagcagcggg tcgagcgcca ccgcgcgcgc
    16681 cagcgccacc cgccgcgcca tgccgcccga gagctcgttg ggatgcagct tcgccgcgcc
    16741 gcgcagcccc accgcctgca gcttcatcag caccaggtcg cgcaccatgc ccgcgggcag
    16801 gtcggtgtgc tcgcgcagcg ggaaggccac gttgtcgaac acgctcatgt cggtgaacag
    16861 cgcgccgaac tggaacagca tgcccatgcg ccgacgcagc gcgtagagct cgctgcccga
    16921 cagccgcgcc acgtcgcgcc cctccacctc cacggtgccg ccggtcgcgc gcagttgccc
    16981 gccgatcagg cgcagcagcg tggtcttgcc gcagccgctg ccgcccatga tggcgaccac
    17041 ctggccgcga tgcacgctca ggtcgatgcc gcgcagcacc tcgcgctcgc cataggcgaa
    17101 gcgcacgtcg cgcagcgaaa ccagcgggga gggatgtggg gcggacacgg tcacgtcgat
    17161 cggccgaatg tgaaaactct tgaatatatc agagatttgc gcccgctggc ggactgatgc
    17221 tgcagcgcag caagcaggtc cgggccattc ccccgcaaga cacctcctcc gctccggtga
    17281 cgaccgcggc acccagaaaa taattccatg cgcttattca cattttcttt caatacgaat
    17341 agcatgtttg catgacattc acattgaccg acctgcgcag gctgctcgcg accgcggcgc
    17401 ttctggcgac cgctgcatgt gcgccgctcg gccgcccgcc cgcactcgag gggcatctcg
    17461 acaagcgcac cgcgatcgag cgcaccgcgc ctgtcggcac ggcgacaaga agcctcgcgc
    17521 cgccgatcgc ggagtcgcct tcttccagcc gaggcgctgc agcgagcgcg gcgacgggcg
    17581 cgcctccgcc cgctccggcc cgcgggcgag cagctcccgc ggtgcgcgga tactcgatcg
    17641 acgttttcga catcccggcc gagcagctgc tgctcgccat cggccgcgac gccgggctgg
    17701 agctcgacct ccatgccgga atcaccggaa acgtcagcct gcaggcccac gaccgcccgc
    17761 tcggcgagct gctggagcgc atcgcccgcc aggtcgacct gcgctatgag ctgcgcggcc
    17821 gccacctgcg cgtgcgcccc gacaccccct acatccgcag ctatgcggtg gactatgtga
    17881 acctgagccg cgacatgcgc ggcaccgtcg ccaccagcac gcagatcgcc acctcgggca
    17941 gcgccggcgg cgccggtcag cgcgcgtctt cgacaggcgg cgctggcaac gcctcgatca
    18001 cccagatcga gacgcgctcg gccaacgact tctggaacac gctcgagcac aacctcgccg
    18061 ccatcctcgc cgggccgggg cgcgcgcccg actgcggggg cgagcgccgc ggcgagtccc
    18121 ccgccggctg ccgcaacgcc gacctgatgc tcaaccgcga gagcggcatc gtcatggcgc
    18181 gcgccaccca gcgccagcac gagcagctgg aggccttcct cgcgggggtg cagcgcgcgg
    18241 cgcggcgcca ggtgatgatc gaagccacga tcgtcgaggt caccctcgcc gacggctacc
    18301 gccagggcat cgactggacc cggctcggcg tgccgagctg gagcgtgcgc ccgcgcggca
    18361 gcggcgacgc cacacccggc cagcccacgc tgagctacct gtcggccaac tccgacatcc
    18421 gtctcgaact gctggagacc ttcggcaccg tcaaggtgct ctccagcccg cgcctgtcgg
    18481 tgctcaacaa ccagaccgcg atgctcaagg tcgtcgagga ggtcgtctac ttcctcgtag
    18541 acagcaccac caccgagtac gacgaccgca agcaggcccg catctccgcg accaccacgc
    18601 cgcaatcggt ctcggtcggc atggtcatgt cggtcacgcc acagatctcc gccgacggcg
    18661 acatcaccct caacgtgcgg ccgaccatct cgggcatctc cggcttcaag gacgacccca
    18721 acccctcgct cggcgacatc cccaaccgcg tgccgcagat ccgcacacgc gaaatcgagt
    18781 ccatgctgcg cctgcgcagc ggcgaggtcg ccgtcctcgg cgggctgatg gaggaccgca
    18841 tcgaccacga caccggccgc atcccgctgt tcggcgacct gcccgtggtc ggcgaactat
    18901 tcacccgccg cgacaacaac gtgcgccgca ccgagctgct catcttcctg cgccccctgc
    18961 tgatcgacga gccgggcatg cgcggcggct acgccgaata cgccggccac ctgcccgacg
    19021 acgacttcct cctcgactcg ccctcgcccg gccagcgcaa cttcccccac ctgccgctgc
    19081 gcccgctgct cgcccccgca ggcgcggtct ccgccgccga aggcttttcg gaagcggcct
    19141 atcccgaccc tgccgatccc cgctctcctg ccgcctcctc cacgtccgcc agcgccgcca
    19201 acgcggatgc cggcacgagc ccgccatgaa cgctccgctc cccatcggcc agatcctcat
    19261 cgccgccggc ctgatcggcg aggaccagct ccgcatcgcg ctccacgagc aacgcggccg
    19321 cgcccggccg ctcggccgcg tgctggtcga actcggcttc gtcagcgagg ccgcgctgcg
    19381 cgaggcgctc gccgcacgca gcggcctgcc ctgcgtcgac ctggccagcg cgctcgccga
    19441 ccccgacgcg atcgcgcgcg tgccgcaggc gctcgcaagg cgccaccgcc tgctgccgct
    19501 ccagtacgac gccgcccgcc accgcctgat cgtcgcgatg gccgacgccc acgacatcgt
    19561 cgccctcgac cgtctgcgtg ccgagctcgg gccggacgtg cacgtcgaac tgcgcctggc
    19621 cggcgacaac gaactcggcc gcgcgatcga acagcactac ggtcaggcga gctcgatcga
    19681 ggacatggtg cgcgagctcg aacgccgcgc cggacagccc atcgctggcg cgcgcgaccc
    19741 gcaactcgtc gtgcgcctgg tcgacgccct gctcgccgag gccgccgcgc gcggcgcctc
    19801 cgacctgcac ctggagcccg aggccggctt cctgcgcgtg cgccaccgca tcgacggcac
    19861 gctgcgccag gtgcgcgcga tgcacaagtc gtgctgggcg gagctcgccg tgcgcatcaa
    19921 ggtgctcgcc ggcatggaca tcgccgagtc gcgcagcccg caggacgggc gcatcggcct
    19981 tgcgctcggc ggccggccga tcgacttccg cgtcgccacc cagcccacct tgcacggcga
    20041 gaacatcgtg ctgcgcatcc tcgaccgcga caagggcatc gtcccgctcg acgcgctcgg
    20101 cctcgacccc gcgcaacgcg ccgccctcga ccgcatcctc gcccgccccg agggcctgat
    20161 cctggtggtc ggtcccaccg gcagcggcaa gaccacgacg ctgtactcca tgctcggcca
    20221 cctcaacacc gaggcggtca acatcatgac tctggaagat ccggtcgagt atccgctcgc
    20281 gctgatccgc cagacccagg tcggcgacgc ctcacggctc gacttcgccg acggcgtgcg
    20341 cgcgctgctg cgccaggacc ccgacgtgat cctgatcggc gagatccgcg acgccgacac
    20401 cgccaccatg gccttgcgcg ccgcactcac cggccaccag gtgttcgcca ccctgcacgc
    20461 caactcgatc ttcggcgcgc tgccgcgcct gcacgagatc ggcatcggcg ccgaactgct
    20521 cgccggcaac ctgtgcggca tcctcgccca gcgcctggtg cgccggctgt gccccacctg
    20581 ccgcgagccg gtgccggacg acgcccccga gcgtcacctg cccggcctcg ccgccgatgc
    20641 cccgccagcg ccgagctggc gcgcgcgcgg ctgccccgaa tgcgacttcc gcggctatcg
    20701 cggccgtctc gcgatcatgg agatcctgcc cttcgacgcc gagctcgacg agctcgtcgc
    20761 acgccgcgcc agcccgggcg agctgcgtgc agccgcacgc acgcgcggcc atcgcagtct
    20821 ggccgaggac ggactgcgcc gggtgctcga cggcagcacc agcctcgccg agctcgcccg
    20881 cgtggtcgac ctctccgcgc tcgcggctac ggcgaagacc gcgcacggag gtgaggcatg
    20941 agcgtgcccc tgcggccgcc cgaagggcag cgcgctcgtc cgccgcggag gatgtcgtgc
    21001 agcgacggga ggaaggcccc atgagcacct ggcgctaccg cgcgctcgct gccgacggcc
    21061 gccgcgtgcg cggcgagctc gatgcggccg acctcggcga actcgagcgc ctgctcgaag
    21121 cgcgcgccct gctgctgatc gacgcccaac cacgcggcgt gcgcggcctg ctgccgcgcc
    21181 ggcgcatcgc gcgccgcgag ctgattccct tctgctacca cctcgagcaa ctcctcgccg
    21241 ccggcgtgcc gcccttcgag agcctggcct tgctgcgcga cgccggcgcc gagccgcggc
    21301 tgcagcaggt gatcggcgca ctgatcgcgg acgtcgagcg cggcctgccg ctgtcgggcg
    21361 ccgccgctcg ccagcccgag gccttctccg cggtcgcggt gagcctgctg cgcgcgggcg
    21421 agcaggccgg ccgcctgccc gaggcgatcc gcgacatcgg cgccgcgctc gcgcaggaag
    21481 aagaactcgc cagccacgcg cgccgcatcg cgatctatcc cgccatcgtc gccacggtgc
    21541 tgctggccgc ggtggtggtc gccctcatcc acgtggtgcc cgagctggag cgtctgctgc
    21601 gcggcaccgg ccagcgcctg ccgctgcaga cgcgcatcct ggtgggactg tccgcggcgg
    21661 tgcgcgactg gggctgggcc atgctgctcg tcacgctcgc cggcgcggcg agcagcgccc
    21721 acgcgctcgc ccgctccgaa gcgctgcgca cccgcgtgca cgccctgctg ttgcaactgc
    21781 cgctggtcgg cgggatcctg cacaagctcg ccctcgcgcg cttcgccgcg ctcttcgcca
    21841 ccctctacgg cgccggcatc aacgtcatcg acgcgctcca caccagcacc ggcgccaccg
    21901 ccaacctcgc cctgcgcgcc ggactgcgcg aggccacggg cgcgatcgag cagggccgcc
    21961 cgatctcggt cgccttcgag gcgacccggg tcttcccgcc cctcgtcatc cgcatgctgc
    22021 gcctgggcga acacaccggc gcgctcgacg acgcgctcgc caaggtcgcc agcctctacc
    22081 gccgcgacgt cgccgagggc atcgcccgcc tgcaggccgc ggccgaaccc gcactcacca
    22141 tcctcatggg cggcctgctg ctgtggatcg cgagcgcggt cctggggccg atctacgagc
    22201 tcatcacccg cctgccggtc tgaccatgcg tcgcgaaccc ctgctcctcg ccgtggctcc
    22261 gtccggcctc ctcgcatgca tcgggcatgg cgacggcatc accctcctcg ctgccttcac
    22321 ctgcggcgag gaggccgcct tcgcaagctg gctcgcccgc cgcccgccgg acgagccctg
    22381 ccggatgttg gtcgacctgc ccgacgagtc ctaccagatc gaggacctgc cgcgcgtgcg
    22441 cggcagcgac cgtcgcgccc tgttcgcacg ccgactcgct cactggttcc ccgagccgcg
    22501 cttcgcacgc gccacgccgc tcggcgccct gcccgacgga cgccagggcg ccgagcgggt
    22561 gcttttcgcc ggaatggaga gcagcaccga cctcctgccc tggctggatc gcctcgcggc
    22621 cgacggacgc cgcccccaaa tcctcgtccc cgccagcgcc ctcctgccac gcctgcccct
    22681 gccaggagca cgccagcgcc gccacggcaa ggcgccgcca cggccgcgcc tgctcgccac
    22741 ccacgggcgc gcgggcctgc gcatttcgct gctggccggc gaacacaccc tgttctcccg
    22801 cctcgtgcgc ggccacgccg actccctcgc cgatccgcaa gcgctggcga cggagctcga
    22861 gcgcacccgc gactacctgg ccgcccagca ccgcatcgcc agcgatgcgc cactcgatcg
    22921 catcgtcgcg gaccttccgg ctcagaccgg gacggagcac gccacactgc ctccgatcgg
    22981 cgtcgccgcc gccgacacgc tcgcgggctg cggactcccg aacagctacg acgcccattt
    23041 gctgctcgcc ctgcgccacg cccccacgac gatcggctgg ccgctcgcgg ccggcgcacg
    23101 gcgctggccc aggctgcccg aaaggcgcgt gctcggcgcg ctcgcgctcg tcgcctcgac
    23161 gatcctggga gccctcgtct ggatggagca ccaggcgcag gtcgaggccg cagccctggc
    23221 cgcgaccgag cgcgcccgtg cggcgcgtgc cgccgcgctt gcggccgagg aggcggaact
    23281 cgccgcacgc gaggccgaac gcgccgcact cgccgccctc gatgcagcgc cgccgcccct
    23341 tccgacccca gcgccggccc ccgccgagcc tgcaccggca gcctgtccgc ctgcctccag
    23401 cccgccgccc gggcccatcg ccaggcgcat cgacggcgtc ctgcgtcgcc cggatggcga
    23461 gatcctgctc tgggtggagg gaacctggca gtccgcgcgc gcactcggcc tgcacccgat
    23521 cgcaggcgac gccgcggtgg tagtggcggc cggccggcgc acgcagctac gcagcggaga
    23581 gctcgtgccg accgccgtcg cgacggtcac ggccgggcct gggcaggcct accccgagcg
    23641 cgacgatgcg gcacccgcag atcgagcagc gggccccgcc gacagcgctg caaggcccag
    23701 tgtcgacatg cgcggagcgc ggccatgatc cggagggccc gcctgccctg ccgggagcgg
    23761 cgccgccagc gggatccgca ctccgctcgc gtgcccgcgc gggtgatgcg tccccggcga
    23821 cccggcgcga tccgcggtca ggccctgatc gccgccctgc tggtgctcac cctgctcgcg
    23881 gcgggcagcc
//
LOCUS       DAOQNN010000010        21672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_828_length_21672_cov_11.839016, whole genome shotgun sequence.
ACCESSION   DAOQNN010000010 DAOQNN010000000
VERSION     DAOQNN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 21672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21672
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_828_length_21672_cov_11.839016"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PKV97_01550"
     CDS             <1..942
                     /locus_tag="PKV97_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB04586.1"
                     /translation="LAKALAQAALAWLLAFVVLASLVWRGSGLRWGEVAAALLAGHTV
                     LAWRAALVTLAVLLAVVGVCVELAPLYHVLGTDKVGQDVLYLALKSVRTALVIGTLTT
                     LVMMPFAVALGIAAGYLGGWVDDAVQYVYTVLNAIPGVLLIAAAVLMMQVVIDTNPHW
                     FDTAAERSDARLLALCFILGITSWTGLARLLRGETLKLRELEYVQAARAFGVRTPAIL
                     RRHILPNLMHIVLIALVMDFSGLVLAEAVLSYVGIGVDPSTISFGTMINMARAELARE
                     PMVWWSLAAAFVFMFVLVLAANLFADVVRDAFDPRRA"
     gene            992..2566
                     /locus_tag="PKV97_01555"
     CDS             992..2566
                     /locus_tag="PKV97_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNB04587.1"
                     /translation="MRALRFDNRFVRELPADPEAENHVRPVHGACYSRVMPTPVRAPR
                     LLAWSREVAHILGLEEADVRSAEFARVFGGNGLLPGMEPYAACYGGHQFGNWAGQLGD
                     GRAITLGESINARGERWELQLKGAGPTPYSRFADGRAVLRSSLREFLCSEAMHHLGVP
                     TTRALSLVGTGETVVRDMLYDGNPRPEPGAVVCRVAPSFIRFGNFEIFASRGEEALLE
                     RLIDFTIARDFPELAAEPDAAARRIRWFDEVCRRTAVLVAHWMRVGFVHGVMNTDNMS
                     ILGLTIDYGPYGWVDDFDPDWTPNTTDAGGRRYRFGNQPFIAHWNLWQLANAIYPVVR
                     EVEPLERALAAYADVHDSSYRDMMRAKLGLAEWRGGEEGDDGLLERLHRLLAAGEVDM
                     SLFFRRLADVDPAAPTLEPLAEAFYDPTRRAAVEAELLAWLRAHGARVLGDGRLAAAR
                     RAEMNRVNPLYVPRNYLAQQAIDAAEGGDMSELEALLEVLRRPYDEQPGRERFAARRP
                     DWARDRPGCSMLSCSS"
     gene            2810..3532
                     /locus_tag="PKV97_01560"
     CDS             2810..3532
                     /locus_tag="PKV97_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB04588.1"
                     /translation="MRPALARKAASPREALARMPILLGSGPEVLDELARGAEFVRLSS
                     SATVFERGSHPSGLFFVCNGAVRLMVSGPEGKDKVVELFEPGGMFGEIGVFTGERYRT
                     WTQAVGAVVLVHVPRERVLAAVAMDNSLSNRMLVAVCARIQRLIDAIGSTVGGPAAVR
                     VASYLLEQLERGARSDGCIVLAAPKKTIASLLNLTPETLSRVLRSLVEEGVLTVSGRR
                     IRVRNRAQLARLLTHDEAVLPL"
     gene            3683..4945
                     /locus_tag="PKV97_01565"
     CDS             3683..4945
                     /locus_tag="PKV97_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HNB04589.1"
                     /translation="MATRKFQQMSVLIMSTTAFTVCFAVWMLFAVIGIPIKEVLALNE
                     TQFGLLAATPVLTGSLIRLPLGMLTDKFGGRIVFFVLMLCTVLPIWLIGHATEYWQFL
                     VAGLFVGFAGGSFSVGIAYCARWFERQNQGFAMGVFGAGNSGAAVTKFVAPTLVVMFG
                     WQMVPQVYAAAMLITAVAFWFFTYDDPEHRVHSSVTFRDQLRVLKDPRVWKYCQYYSI
                     VFGGYVGLSLWLTKYYVNEFGMGIQAAALLAACFSLPGGVLRAVGGWLSDRYGAHKVT
                     WWVMWASWICLFLLSYPNTDFTVQTVEGPRTFSIHLNVWVFTILLFIVGIAFAFGKAS
                     VFKYISNEFPKEIGAVSGIVGLVGGMGGFLLPIMFGALVDLTGVRSSAFMLLYGVTWV
                     SLIWMYLTEVRRVDMFDGHDTAVDSSKA"
     gene            4973..6628
                     /locus_tag="PKV97_01570"
     CDS             4973..6628
                     /locus_tag="PKV97_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB04590.1"
                     /translation="MQASNVAAPSPAITSSVDLVDWRPEDEAFWKEKGSRIANRNLWI
                     SIPSLLCGFAVWLMWGIITVQMANAGFPFAPDQLFSLAAIAGLSGATTRIPASFFIRI
                     CGGRNTVWLTTALLMIPAVGTGIALQSLETPLWVFQLMALLSGMGGGNFACSMANIST
                     FFPKRLQGTALGLNAGLGNFGVTTSQLLIPAVMTMGVFGALGGDAIPLVKDSATLIGK
                     IAAGTPTYVQNAGFVWLLLLVPLAVVGWFGMNNLLTVSPNIGSTGAALGKIILFYGIA
                     LVVSAIGLYLYLPSGLGVLAGGGMWIMLPLVILGTLYGMKFAASGEMKTNLDKQFAIF
                     RNKHTWSMTALYVVTFGSFIGFSMALPLSITVIFGNTHVLDPATQAWVHAKNPNAPSA
                     LMFAWIGPFVGALIRPVGGWISDKLGGSIVTQWISIVLVIASAVTGYVMFLAYHSATP
                     EQYFFMFMALFVLLFAASGIGNGSTFRTVGFVFDKDERGPVLGWTSAVAAYGSFIAPV
                     VIGGQIKAGTPEYAMYGFAVFYALCVVLNWWFYLRKDAYIQNP"
     gene            6718..10473
                     /locus_tag="PKV97_01575"
     CDS             6718..10473
                     /locus_tag="PKV97_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HNB04591.1"
                     /translation="MSHFLDRLRFFDKVKETFSNGHGIVTNEDRNWEDAYRNRWRHDK
                     VVRSTHGVNCTGGCSWKIFVKNGLVSFEMQQTDYPRTREDLPNHEPRGCQRGASFSWY
                     LYSPHRIKYPMIRGRLLDLYRAERKAGRDPVEAWEAIQKDPAKREKYVHVRGLGGFVR
                     TSWDEVTEIVAAANVYTIKKWGPDRIYGFSPIPAMSMISYAAGARYLSLIGGACGSFY
                     DWYCDLPAASPQTWGEQTDVPEAADWYNSTYLIITGANLPMTRTPDAHFATEVRYKGT
                     KIVSMAPDYAEYVKFADLWMPVKQGTDAAAFLAMGHVALKEFLVEKQDPYFTEYARKY
                     TDLPMQVVLRKHGEGYVSDRNLRASDFDNNLGESNNPEWKTIVFDKKSGRFIAPNGSI
                     GFRWGEDGKWNLLEKAGADQAETEAELTCIGSQDEVVSVGFPHFVPGEPELLYRNVPA
                     RRLKLASGEEAFVTSVFDMQVAQYGIDRGLGGANVASSYDDANVAYTPAWAAKQTGVK
                     AADLERTGREFAENASKTKGKSMVIMGAAINHWYHNDLAYRSIMNLLHMCGCVGQTGG
                     GWAHYVGQEKLRPQAGWAPIAFALDWQRPPRHMNSTSFWYFHTDQWRYETVDADSLLS
                     PAGPCRNKGSSLADYNVKAVRMGWLPSAPHFNRNPIELAEEAARAGATDEASVSKYVV
                     EQLKSGALDVSFADPDAEENWPRNLIVWRANLIGSSAKGHEYFLKHLLGAQNGVMQEG
                     GAGNNCKEVKWREHGPTGKLDLMVDINFRLNSTGAYSDIILPTATWYEKNDLNTTDMH
                     PFIHPLGEAVTPGWESKSDWQIFRTLAKTFTALGAKHLGTRKDVVSLPMQHDSPMELA
                     TPMGVGKDWKHGECDPIPGKTMPMLKLVERDYAQIYNKYIALGPLMVKLGNNVKGIDW
                     DTTQEYEELKKCNYPVQVEGVSKGMPSLEEDIYVCDSVMRMAPETNGEVAHKSWSALS
                     KKTGIDHHHLYAGRHEDKITFRDIVAQPRKIITAPTWSGIESETVSYTAGYTNIHEHI
                     PFRTLTGRAHFYQDHEWFLDFGEGFCAYKPPVDLKAHEKVPDSVRNKPHLTLSWITPH
                     SKWGIHSSYQDNLRMLNLFRGGPYVWLSEEEAATIGIRDNDWVEAINANGATVARAVV
                     SQRIPRGMAIMYHAQEKNVNVPGSNTTGKRGGILNSVTRVIVKPTNMVGGYAQLAYSF
                     NYYGTVGCQRDEVVVIHKIEDQDIDWLERPLTPEREAQRNPVGIGKK"
     gene            10509..12080
                     /gene="narH"
                     /locus_tag="PKV97_01580"
     CDS             10509..12080
                     /gene="narH"
                     /locus_tag="PKV97_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HNB04592.1"
                     /translation="MKIRAQFAMVFNLDKCIGCHTCSVTCKNVWTNRKGVEYAWFNNV
                     ESKPGIGYPKEWENQDKWKGGWTKLNGKLELKAGGRLKKIVQIFSNPNLPEIDDYYEP
                     FTYDYARLQNAPLSEAAPTARPVSQITGEKMDKITWGPNWEDDLAGEFASRARDKNFS
                     NMEKEMYKQFENTFHMYLPRICNHCINPACVASCPSGALYKREEDGIVLADQDRCRGW
                     RMCISGCPYKKVFFNWESSKAEKCVGCYPRVENGMPTVCSESCVGRIRYNGIILYDAD
                     KISAAASADNVQDLYQEHLDMFVDPFDPAVIEQAKKDGITDDWILAAQKSPIYKMAVQ
                     WKIAFPLHPEYRTLPMIWYVPPLSPVQSQIDQKALPTEADGVIPKASAMRLPVKYLAN
                     LMTAGKEQPIIDALNRMIAMRSYQRSVHVEGTPDTRALEAVGMTEDMAKEMYRYLAIA
                     NYEDRFVIPTSKEEMRLEDYHGFQGQAGFSFGSASSVGISPNSLFPERRKESMEPRKP
                     VPHADELHDVTGERY"
     gene            12093..12641
                     /gene="narJ"
                     /locus_tag="PKV97_01585"
     CDS             12093..12641
                     /gene="narJ"
                     /locus_tag="PKV97_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HNB04593.1"
                     /translation="MQTYKLLAMLLDYPGSEIVGDLRAAVSEQGGARGLVRSVDTDQV
                     FTEAEHGSIAGFIDWMLAQDQTELEGRFVKTFDLTPEHSLHLTHHVFGDDKNRGPALI
                     DLSEFYKSYGLQHDEHEIPDYLPMMMEFVSQLGETEARVFLTDAVKVFNVLATNLEKA
                     ESPYAALVRVIENHSSLARAAA"
     gene            12652..13344
                     /gene="narI"
                     /locus_tag="PKV97_01590"
     CDS             12652..13344
                     /gene="narI"
                     /locus_tag="PKV97_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNB04594.1"
                     /translation="MSLTNLFFGVYPYIALTVFVIGSWIRYDNEQYTWKTDSSQLLSK
                     QGMMLASNLFHVGVIMIFFGHFAGLVLPHGLWLGLGISDLAHQWIAIIAGVIFGIMCL
                     VGGSMLWYRRMYNPRVRINSRRSDVFIITWLMATLILGLLTVPFSIGHAVKGDPAVML
                     ALAEWVKSILLLNPQPELMTKVDPIFRAHIFFGMTVFLVFPFTRLVHILTAPFSYLTR
                     AYQIVRTKRRLA"
     gene            13449..15392
                     /locus_tag="PKV97_01595"
     CDS             13449..15392
                     /locus_tag="PKV97_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNB04595.1"
                     /translation="MFIRGSGLSVKITGMLLGFFLVALAAIALTLFISWRLEGAAAAI
                     NDAGSLRMRLYRVAHHLARADLHTSDRQGFAGDLRARVDDFDRILGVLRRGDPSRPLF
                     VPREAGIQQVLSGMSEEWSVQLRPILLAIAADPDPANLHRRGLEFDRAARDYVEDIND
                     FVLRMEQSYARNTNILRTSQVILVALAVIGTILLLRFFFLTVIRPVFELHEGVKRMGQ
                     EDFDARVPVLSQDEFGELSAGFNRMAAHLQNVYDTLEERVVAKTRSLEEKNRELEILY
                     EIVAFLREPNDVDTLCKGFLERVQKTMGASASSARLLDLESRNLCMIVHDGLGDDFLD
                     REEVLACGECVCGEAAQGSGLSLTLDVRNAALALTRDACRRAGFRTVSATSITASKRA
                     LGVFNLYFDQSDAIGDADRQLLEALGQQLGIAIENQRLHARERELAVSEERNLLAREL
                     HDSIAQGLAFLNLQVQMLERALAENRAADVSTTVEMLRRGIQESYEDVRELLVHFRTR
                     VEQQDLDAAIAAALRRLAEQTGIATDLDVQGDGAPLDPEAETQVLYIIQEALSNVRKH
                     AGASTVSVGVRRGRDGLAVTVRDDGVGFAEDRDASDSEAHIGLQIMRERAMRIGGRFA
                     VRSSPGRGTEIRLELPRTNEVLA"
     gene            15392..16123
                     /locus_tag="PKV97_01600"
     CDS             15392..16123
                     /locus_tag="PKV97_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB04596.1"
                     /translation="MAEEKIRVLLVDDHTLFRSGIRSLLQSYVCFEVVGEAGDGREGI
                     RLAGQLRPDVVLLDNNMPGLSGREAARLLLEEVPETRVLMLTVSEDADDLIETLRAGA
                     CGYLLKNIQAETLVSAIKTAAQGESVVSPQMMGKLLSGVRSSASRAAMGGAIAPAPGA
                     LARSAPAAGAEPAELEKLSPREREILRFIARGQSNKEIARALELAESTVKIHVQNMLR
                     KLNLSSRVQAAVFAVENGLGQGPEG"
     gene            complement(16161..16568)
                     /locus_tag="PKV97_01605"
     CDS             complement(16161..16568)
                     /locus_tag="PKV97_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNB04597.1"
                     /translation="MNTPLDKSEFEALADFRYQLRRFLRFSELLTRRHGITNLQYLLL
                     LQVKGYPGREWACIGELAERLQAHQHGVVSLVSRCEKLGLVERRPGREDRRAVEVHLT
                     AEGERLAATIALHHREEVKLLGGVFRDLASQLR"
     gene            16746..17318
                     /locus_tag="PKV97_01610"
     CDS             16746..17318
                     /locus_tag="PKV97_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNB04598.1"
                     /translation="MDVMQFPRMVVSSRDGWDLVARERPPMDWLFRRLVLPMSLLPPL
                     MILLASGGVGARVFPDAGFGNWLIAAVLFFVAEHFSVPIMANSIRQASMAKDGSSRLE
                     DAYTVAAIAPVPLWLSSLAVVFGSAWFVVLVGLVALGASAMLIRHGVDRLLGIDEDVE
                     ASEVAMQVTSFGVLTWLVLLAVAGGAVTLL"
     gene            complement(17325..17771)
                     /gene="smpB"
                     /locus_tag="PKV97_01615"
     CDS             complement(17325..17771)
                     /gene="smpB"
                     /locus_tag="PKV97_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNB04599.1"
                     /translation="MSIIENRKALHDYFIEEKHEAGIVLEGWEVKAIRAGRSNIKESY
                     VVIRAEEIFVFGMHITPLSTASSHVRHDPTRTRKLLLHKKEIARLIGKVERAGYTLVP
                     LDLHYKNGLVKLEIGLAKGKKLHDKREDEKKKDWEREKQRLMRVKA"
     gene            17894..18346
                     /locus_tag="PKV97_01620"
     CDS             17894..18346
                     /locus_tag="PKV97_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HNB04600.1"
                     /translation="MAEVNKLVLIEFTPAQMFELVDRCEEYPLFLPWCGGVDLIGRTE
                     TITAATLHINYHGIKAHFSTENAKRYPQEMKLRLTDGPFTHLDGHWRFTALGETACKV
                     EFQLRYQFASKLLEKVLGPVFNHIANTFVDSFVKRAGQVYRAGEGSAA"
     gene            18343..18669
                     /locus_tag="PKV97_01625"
     CDS             18343..18669
                     /locus_tag="PKV97_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNB04601.1"
                     /translation="MSGMINVEVVYALPQRQELVHVRLAEGASVRDALEASGLLQKHP
                     EIQPDRANKLGIYARLVKLDTEVRDRDRVEIYRPLIADPKAVRKKRADEGKVMKKGGG
                     PAEAAE"
     gene            complement(18756..19256)
                     /locus_tag="PKV97_01630"
     CDS             complement(18756..19256)
                     /locus_tag="PKV97_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNB04602.1"
                     /translation="MTHPFAPVAILALLLTALPAAAQVYSWKDKDGRIHYGDMPPAGV
                     EATVIRGARPGAAARKTGETAPSADMPPAATPTSPSSLAEREQAFRERRAAAAEAEAK
                     AAREAESRGETERFCTDAQHELAALRGGQRMVRFNAKGEREFLDDRARAEQAEALERK
                     IAEHCR"
     gene            19339..20802
                     /gene="guaB"
                     /locus_tag="PKV97_01635"
     CDS             19339..20802
                     /gene="guaB"
                     /locus_tag="PKV97_01635"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNB04603.1"
                     /translation="MRVIQKALTFDDVLLVPAHSTVLPRDVSLQSQVTRRIRLNVPLV
                     SAAMDTVTESRLAIALAQEGGIGVVHKNLTPAQQAAEVLKVKRHESGILKDPITIPPT
                     MTVGEVIALQRQNRFSGVPVVEGGKVVGIVTNRDTRFETKLDQLVSEIMTPQDRLVTV
                     REGASLEEARELLRVHRLERVLVLNDAGELCGLITVKDMMKSTEHPLAAKDEHGRLRV
                     AAAIGVGAGTEERAERLADAGVDMIVVDTAHGHSQGVLDRVGWVKKHFPHIEVVGGNI
                     ATADAARALVDAGADGVKVGIGPGSICTTRIVAGVGVPQISAIDNVANALLGTGVPMI
                     ADGGIRFSGDIAKAIAAGADVVMLGGLFAGTEEAPGETVLFQGRSYKSYRGMGSLGAM
                     EKGAADRYFQEENTGNIDKLVPEGIEGRVPYKGSVTAVIHQLVGGLRASMGYLGCADI
                     PTMHARAEFVQISSAGMRESHVHDVQITKEAPNYQIS"
     gene            20875..>21672
                     /gene="guaA"
                     /locus_tag="PKV97_01640"
     CDS             20875..>21672
                     /gene="guaA"
                     /locus_tag="PKV97_01640"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNB04604.1"
                     /translation="MAHQKILILDFGSQVSQLIARRVREQQVYCELHPFDVSDEFVRN
                     FGAQGVILSGGPNSVYEAEDWRAPQAVFELGVPVLGICYGMQTMASQLGGKVESSAKR
                     EFGYAEMRARGHSKLFAGIEDRSNAEGHGLLDVWMSHGDKVTALPPGFKIIGSNESTP
                     IAAMADEARGFYGVQFHPEVTHTIKGKEMIGRFVHDICGCGHDWNMPDYIEEAVQKIR
                     EQVGDEEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEG"
BASE COUNT         3559 a         7233 c         7151 g         3729 t
ORIGIN      
        1 ctggcgaagg cgctcgcgca ggcggcgctc gcgtggctgc tcgccttcgt cgtgctcgcc
       61 agcctggtat ggcgcggcag cgggctgcgc tggggcgagg tggccgcggc cttgctggcc
      121 ggacacaccg tgctggcctg gcgcgcggcg ctggtgacgc tggcggtgct gctcgcggtg
      181 gtgggggtgt gcgtggagct ggcgccgctc taccacgtgc tcggcaccga caaggtcggg
      241 caggacgtgc tctacctggc gctcaagagc gtgcgcacgg cgctggtgat cggcaccctg
      301 acgacgctgg tgatgatgcc cttcgcggtc gcgctcggca tcgccgcggg ttaccttggc
      361 ggctgggtgg acgatgccgt gcagtacgta tacaccgtgc tcaacgcgat ccccggagtg
      421 ctgttgatcg ccgccgcggt gctgatgatg caggtcgtca tcgacacgaa tccgcactgg
      481 ttcgacaccg cagccgagcg ctcggatgcg cgcctgctgg cactgtgctt catcctcggc
      541 atcaccagct ggacggggct ggcgcgcctg ttgcgcggcg agacgctcaa gctgcgcgag
      601 ctcgagtacg tgcaggcggc gcgcgccttc ggggtgcgca ccccggcgat cctgcgccgc
      661 cacatcctgc ccaacctgat gcatatcgtg ttgatcgcgc tggtgatgga cttctccggg
      721 ctggtgctgg ccgaggcggt gctgtcctac gtcggcatcg gcgtcgaccc gtcgacgatc
      781 agcttcggca ccatgatcaa catggcgcgt gccgaactcg cgcgcgagcc gatggtctgg
      841 tggtcgttgg cggcggcctt cgtgttcatg ttcgtgctcg tgctcgccgc caacctgttc
      901 gccgacgtgg tgcgcgacgc cttcgacccg cgccgcgcct gagcgcgccc tggcccgcac
      961 ccgccgggcc gaccatgacg aggatttccc gatgagagcg cttcgcttcg acaaccgctt
     1021 cgtgcgcgag ctgcccgccg atcccgaagc ggagaaccac gtccgtccgg tccatggcgc
     1081 ctgctactcg cgggtgatgc cgacgccggt gcgggcgccg cgcctgctgg cgtggtcgcg
     1141 cgaggtggcg cacatcctgg gcctggagga ggccgacgtg cgcagcgccg agttcgcccg
     1201 cgtcttcggc gggaacggcc tgctgcccgg catggagccc tacgccgcct gctacggcgg
     1261 ccaccagttc ggcaactggg cggggcagct cggtgacggg cgcgcgatca cgctcggcga
     1321 gtcgatcaat gcgcgtggcg aacgctggga gctgcagctc aagggcgccg ggccgacgcc
     1381 gtattcgcgt ttcgccgacg gccgcgcggt gctgcgctcc tcgctgcgcg agttcctgtg
     1441 cagcgaggcg atgcaccacc tgggcgtacc gaccacgcgc gcgttgagcc tggtgggaac
     1501 cggcgagacc gtggtgcgcg acatgctcta cgacggcaat ccccgacccg agccgggcgc
     1561 ggtggtgtgc cgggtggcgc cgtccttcat ccgcttcggc aacttcgaga tcttcgcctc
     1621 gcgcggcgag gaggcgctgc tcgagcgcct gatcgacttc accatcgcgc gcgatttccc
     1681 cgaactcgcc gccgagcccg atgcggcggc gcggcgcatc cgctggttcg acgaggtctg
     1741 ccggcgcacc gcggtgctgg tcgcgcactg gatgcgggtg ggcttcgtgc atggggtcat
     1801 gaacacagac aacatgtcca tcctcggcct caccatcgat tacggcccct acggctgggt
     1861 cgacgacttc gaccccgact ggacgcccaa taccaccgac gccggcgggc gccgctaccg
     1921 cttcggcaac cagccgttca tcgcgcattg gaacctgtgg cagctcgcca acgcgatcta
     1981 tccggtggtg cgcgaggtcg agccgctgga gcgcgcgctc gccgcgtacg cggacgtcca
     2041 tgattcgagc tatcgcgaca tgatgcgcgc caagctcggc ctcgccgaat ggcgtggtgg
     2101 cgaggagggc gacgacggcc tgctcgagcg cctgcaccgc ctgctcgcgg caggcgaggt
     2161 ggacatgagc ctgttcttcc gccgcctcgc cgacgtcgat ccggcggcgc ccacgctgga
     2221 gccgctcgcc gaggccttct acgaccccac gcgccgcgcc gcggtggagg cggagctgct
     2281 cgcctggttg cgagcgcatg gcgcgcgcgt gctcggcgac ggtcggctgg ccgcggcgcg
     2341 acgggcggag atgaaccgcg tcaatccgct ctacgtgccg cgcaactacc tggcccagca
     2401 ggcgatcgac gccgcggagg gtggggacat gagcgagctc gaggccctgc tcgaggtact
     2461 gcgccgaccc tacgacgagc agcccgggcg cgagcgcttc gcggcgcgcc ggccggactg
     2521 ggcgcgcgat cgccccggct gctccatgct ctcctgcagt tcctgaacgt gcaggcggca
     2581 tcgcgcgtgg tcgccgccgg gcgtgcctgc gccatccggg ttcgccgctt cccgagggct
     2641 ccttgaggcg agggtagtcc tttcggcgta gcgctccgtc gatcggccaa tggccagcgg
     2701 ccgtgccagg gtctaggatg gaactggagg aggaaccgtc gcgacccatg ccggcggata
     2761 aaacagacct gtgtccacca tcgtcctgac tcatgcggag gagtcgcgcg tgcgtcctgc
     2821 gctcgcgcgc aaggccgctt cgcctcgcga ggccttggcg cgcatgccga tcctgctcgg
     2881 cagcgggccc gaggtgctcg acgagcttgc gcgcggcgcg gagttcgtcc gcctgtcctc
     2941 gtcggccacg gtgttcgagc gcggctccca ccccagcggc ctgttcttcg tctgcaacgg
     3001 cgcggtccgc ctgatggtca gcgggcccga gggcaaggac aaggtggtcg agctcttcga
     3061 gccgggcggc atgttcggcg agatcggcgt gttcaccggc gagcgctatc gaacgtggac
     3121 gcaggcggtc ggcgcggtcg tgctggtgca tgttccgcgc gagcgcgtgc tggccgcggt
     3181 ggcgatggac aattcgctct ccaaccgcat gctggtcgcg gtgtgcgcgc gcatccagcg
     3241 cctgatcgac gcgatcggca gcaccgtcgg cggcccggcg gcggtgcgcg tggcgagcta
     3301 tctgctcgaa cagctcgagc gcggtgcgcg cagcgatggt tgcatcgtcc tggccgcgcc
     3361 caagaagacc atcgcctcct tgctcaacct cacgcccgag acgctctcgc gggtgttgcg
     3421 cagcctggtc gaggagggcg tgctcaccgt gagcggccgt cgcatccgcg tgcgcaaccg
     3481 tgcgcagctc gcccgcctgc tgacgcacga cgaggcggtc ctacccctct gagctcgtcc
     3541 gggctagtca ctaaggacta gtccaccttc gtgccggcag cgccgatccc gtgcttttgg
     3601 cgaatggtgc ggggcgccgc ccgagcctat cctcaccccg gaaaattcgt gaccgtcgga
     3661 cctttccgga gcgcagtggg aaatggctac tcgcaagttt cagcagatgt ccgtgctcat
     3721 catgagcacc accgcattca ccgtctgttt tgccgtgtgg atgctgttcg cggtgatcgg
     3781 tatcccgatc aaggaggttc tcgccctcaa cgagacccag ttcggcctgc tggcggctac
     3841 cccggtgctg accggatcgc tgatccgtct gcccctcggc atgctcaccg acaagttcgg
     3901 cggccgcatc gtcttcttcg tgctgatgct gtgcaccgtg ctgccgatct ggctgatcgg
     3961 ccatgcgacc gaatactggc agttcctcgt cgccggcctc tttgtcggct tcgcgggcgg
     4021 ctccttctcg gtcggcatcg cctactgcgc gcgctggttc gaacgccaga accagggctt
     4081 cgcgatgggg gtgttcggtg ccggcaactc gggcgccgcg gtcaccaagt tcgtcgcgcc
     4141 cacgctggtg gtgatgttcg gctggcagat ggtgccgcag gtctatgcgg cggcgatgct
     4201 gatcaccgcg gtcgcgttct ggttcttcac ctacgacgac cccgagcacc gggtgcactc
     4261 ctcggtgacc ttccgcgacc agctgcgcgt gctcaaggat ccgcgcgtgt ggaagtactg
     4321 ccagtactac tcgatcgtgt tcggcggcta cgtcggcctg tcgctgtggc tcaccaagta
     4381 ctacgtcaac gagttcggca tgggcatcca ggcggcggcg ctgctcgctg cctgcttctc
     4441 cctgcccggc ggcgtgctgc gcgcggtggg cggctggctg tccgaccgct acggcgcgca
     4501 caaggtcacc tggtgggtga tgtgggcgtc gtggatctgc ctgttcctgc tctcctatcc
     4561 gaacaccgac ttcaccgtgc agaccgtcga ggggccgcgc accttcagca tccacctcaa
     4621 cgtgtgggtg tttacgatcc tgctcttcat cgtcggcatc gccttcgcct tcggcaaggc
     4681 ctcggtgttc aagtacatct ccaacgagtt ccccaaggag atcggcgcgg tctccggcat
     4741 cgtcggcctg gtgggcggca tgggcggctt cctgttgccg atcatgttcg gcgccctggt
     4801 cgacctcacc ggcgtgcgct cgagcgcctt catgctgctc tacggcgtga cctgggtttc
     4861 cctgatctgg atgtacctga cggaagtccg tcgtgtcgac atgttcgacg gtcacgacac
     4921 cgcggtcgac tcgtccaagg cctgattcgc aagacaacga ggagtgggtt tcatgcaagc
     4981 aagcaatgtc gcggcgccga gcccggcgat caccagcagc gtcgacctcg tcgattggcg
     5041 ccccgaggac gaggccttct ggaaggagaa gggctcgcgg atcgccaacc gcaacctgtg
     5101 gatctccatc cccagcctgc tgtgcggctt cgccgtgtgg ctgatgtggg ggatcatcac
     5161 ggtgcagatg gccaacgcgg gcttcccctt cgcccccgac cagctcttct cgctcgccgc
     5221 gatcgccggc ctgtcgggtg ccaccacgcg catcccggcc tccttcttca tccgcatctg
     5281 cggcggtcgc aacacggtgt ggctgaccac cgcgctgctg atgatccccg ccgtgggtac
     5341 cggcatcgcg ctgcagagtc tggagacgcc gctgtgggtg ttccagctca tggcgctgct
     5401 gtcgggcatg ggcggcggca acttcgcctg ctcgatggcc aatatctcca ccttcttccc
     5461 caagcgcctg cagggcaccg cgctcggcct caacgccggc ctgggcaact tcggcgtgac
     5521 cacctcgcag ctgctgattc cggcagtgat gaccatgggg gtgttcggcg cactcggcgg
     5581 cgacgcgatc ccgctggtca aggattccgc cacgctgatc ggcaagatcg ccgccggcac
     5641 gccgacctac gtgcagaacg ccggcttcgt ctggctgctg ctgttggtgc cgctcgcggt
     5701 ggtcggctgg ttcggcatga acaacctcct caccgtgtcg cccaacatcg gctccacggg
     5761 cgcggcgctc ggcaagatca tcctgttcta cggcatcgcc ctggtggttt cggcgatcgg
     5821 tctctacctc tacctgccga gcggcctcgg cgtgctcgcg ggcggcggga tgtggatcat
     5881 gctgccgctg gtcatcctcg gcacgctgta cggcatgaag ttcgccgcca gcggcgagat
     5941 gaagaccaat ctcgacaagc agttcgcgat cttccgcaac aagcacacct ggtcgatgac
     6001 cgcgctctac gtggtgacct tcggctcctt catcggtttc tcgatggcgc tgccgctgtc
     6061 gatcacggtg atcttcggca acacccacgt gctcgatccg gccacccagg cctgggtgca
     6121 tgcgaagaac ccgaacgcgc cctccgcgct gatgttcgcc tggatcggcc ccttcgtcgg
     6181 cgcgttgatc cgaccggtgg gcggctggat ctcggacaag ctgggcggct cgatcgtgac
     6241 ccagtggatc tcgatcgtgc tcgtgatcgc ctccgcggtg accggctacg tgatgttcct
     6301 cgcctaccac tcggcgacgc cggaacagta cttcttcatg ttcatggcgc tgttcgtgct
     6361 gctgttcgcg gcctccggca tcggcaacgg ctcgaccttc cgtaccgtgg gcttcgtgtt
     6421 cgacaaggac gagcgcggcc ccgtgctggg ctggacctct gcggtggcgg cctacggctc
     6481 cttcatcgct ccggtggtga tcggcggcca gatcaaggcc ggcacgcccg agtacgcgat
     6541 gtacggcttt gcagtgttct acgccctgtg cgtggtcctc aactggtggt tctacctgcg
     6601 caaggacgcg tacatccaga acccctgatt cccgcccgac cgacggattc cctttttcaa
     6661 ccagactttt accgatcgcg cttgacgaac gaagcgacga cttgagagga gcaaaccatg
     6721 agtcattttc tcgatcgcct gaggtttttc gacaaggtca aggagacctt ctcgaacgga
     6781 cacggtatcg tcaccaatga agaccgcaac tgggaggatg cctaccgcaa ccgctggcgc
     6841 cacgacaagg tggtgcgctc cacccacggt gtgaactgca ccggcggctg ttcctggaag
     6901 atcttcgtca agaacggcct ggtgtcgttc gagatgcagc agaccgacta cccgcgcacc
     6961 cgcgaggacc tgcccaacca cgagccgcgc ggctgccagc gtggtgcctc gttctcgtgg
     7021 tacctgtact cgccgcaccg catcaagtac ccgatgatcc gcggccgcct gctcgacctg
     7081 taccgcgccg agcgcaaggc cggacgcgac ccggtcgagg cctgggaggc gatccagaag
     7141 gatcccgcca agcgcgagaa gtacgtgcac gtgcgcggcc tgggtggctt cgtgcgcacc
     7201 agctgggacg aggtcaccga gatcgtcgcc gcagccaacg tctacacgat caagaagtgg
     7261 ggcccggacc gcatctacgg tttctcgccg attcccgcga tgtcgatgat ctcgtacgcc
     7321 gccggcgcgc gttacctgtc gctgatcggt ggtgcctgcg gctcgttcta cgactggtat
     7381 tgcgacctgc cggccgcgtc gccgcagacc tggggcgagc agaccgacgt gcccgaggcc
     7441 gccgactggt acaactcgac ctatctgatc atcaccggcg ccaacctgcc gatgacccgc
     7501 accccggacg cccacttcgc caccgaagtg cgctacaagg gcaccaagat cgtgtcgatg
     7561 gcgccggact acgccgagta cgtcaagttc gccgacctgt ggatgcccgt caagcagggc
     7621 accgacgccg ccgccttcct ggccatgggc cacgtcgcgc tgaaggaatt cctcgtcgag
     7681 aagcaggacc cgtacttcac cgagtacgcg cgcaagtaca ccgacctgcc gatgcaggtc
     7741 gtgctgcgca agcacggcga gggctatgtt tcggatcgca acctgcgcgc ttccgacttc
     7801 gacaacaacc tcggcgagtc caacaacccc gagtggaaga ccatcgtctt cgacaagaag
     7861 agcggccgct tcatcgcgcc caacggctcg atcggcttcc gctggggcga ggacggcaag
     7921 tggaacctgc tcgagaaggc cggcgccgac caggccgaga ccgaagcgga gctcacctgc
     7981 atcggcagcc aggacgaggt cgtctcggtg ggcttcccgc acttcgtgcc gggtgaaccc
     8041 gagctgctgt accgcaacgt gccggcgcgt cgcctcaagc tcgccagcgg cgaagaagcc
     8101 ttcgtgacct cggtgttcga catgcaggtc gcgcagtacg gcatcgaccg cggcctgggt
     8161 ggcgccaacg tggcctcgtc ctacgacgac gccaatgtcg cctacacccc ggcctgggct
     8221 gccaagcaga ccggcgtgaa ggccgccgac ctcgagcgta ccggccgcga gttcgccgag
     8281 aacgcctcga agacgaaggg caagtccatg gtgatcatgg gcgctgcgat caaccactgg
     8341 taccacaacg acctcgccta ccgctcgatc atgaacctgc tgcacatgtg cggctgcgtg
     8401 ggtcagaccg gtggtggctg ggcgcactac gtgggccagg aaaagctgcg tccgcaagcg
     8461 ggctgggcgc cgatcgcgtt cgcgctcgac tggcagcgtc cgccgcgcca catgaactcg
     8521 acctcgttct ggtacttcca caccgaccag tggcgctacg agacggtgga tgccgactcg
     8581 ctgctgtcgc ccgccggtcc ctgccgcaac aagggttcct cgctcgccga ctacaacgtc
     8641 aaggcggtgc gcatgggctg gctgccgtcc gccccgcact tcaaccgcaa cccgatcgaa
     8701 ctcgccgaag aagccgcgcg tgcgggtgcg accgacgagg cgagcgtgtc gaaatacgtg
     8761 gtcgaacagc tcaagtcggg cgcgctcgac gtgtccttcg ccgacccgga tgccgaggaa
     8821 aactggccgc gcaacctgat cgtgtggcgc gccaacctga tcggctcgtc ggccaagggc
     8881 cacgagtact tcctcaagca cctgctgggc gcgcagaacg gcgtgatgca ggaaggcggc
     8941 gcgggcaaca actgcaagga agtgaagtgg cgcgagcatg gcccgaccgg caagctcgac
     9001 ctgatggtgg acatcaactt ccgcctcaac tcgaccggcg cgtactccga catcatcctg
     9061 ccgacggcga cctggtacga gaagaacgac ctcaacacca ccgacatgca ccccttcatc
     9121 cacccgctgg gtgaggcggt gaccccgggc tgggagtcga agtccgactg gcagatcttc
     9181 cgcactctgg cgaagacctt caccgcgctc ggcgccaagc acctcggcac ccgcaaggac
     9241 gtggtctcgc tgccgatgca gcacgactcg ccgatggaac tcgcgactcc gatgggcgtg
     9301 ggcaaggact ggaagcacgg cgagtgcgat ccgatcccgg gcaagaccat gccgatgctc
     9361 aagctcgtcg agcgcgacta cgctcagatc tacaacaagt acatcgcgct cggcccgctg
     9421 atggtgaagt tgggcaacaa cgtcaagggc atcgactggg ataccaccca ggagtacgaa
     9481 gagctcaaga agtgcaacta cccggtgcag gtcgaaggtg tttcgaaggg catgccgtcg
     9541 ctcgaggaag acatctacgt ctgcgactcg gtcatgcgca tggcgcccga gaccaacggc
     9601 gaagtggcgc acaagtcgtg gagcgcgctg tcgaagaaga ccggcatcga tcaccaccac
     9661 ctgtacgccg gccgtcacga ggacaagatc accttccgcg acatcgtcgc ccagccgcgc
     9721 aagatcatca ccgcgcccac ctggtccggc atcgagtcgg agaccgtgtc ctacaccgcg
     9781 ggctacacca acatccacga gcacattccg ttccgtaccc tgaccggtcg tgcgcacttc
     9841 taccaggacc acgagtggtt cctcgacttc ggtgaaggct tctgcgccta caagccgccg
     9901 gtcgacctca aggcccacga gaaggtgccc gacagcgtgc gcaacaagcc gcacctgacc
     9961 ctgtcgtgga tcaccccgca ctcgaagtgg ggcatccact ccagctacca ggacaacctg
    10021 cgcatgctca acctgttccg cggcggcccg tacgtctggc tgtccgagga agaggccgcg
    10081 accatcggca tccgcgacaa cgactgggtc gaggcgatca acgccaacgg cgccacggtg
    10141 gcccgtgcgg tggtgtcgca gcgcatcccg cgtggcatgg cgatcatgta tcacgcccag
    10201 gaaaagaacg tgaacgtgcc cggctcgaac acgaccggca agcgtggcgg catcctgaac
    10261 tcggtcaccc gcgtcatcgt caagcccacc aacatggtcg gcggctatgc gcaactggcc
    10321 tacagcttca actactacgg caccgtcggt tgccagcgtg acgaagtcgt ggtcatccac
    10381 aagatcgagg accaggacat cgactggctg gagcgtccgc tcacgcccga acgcgaagcc
    10441 caacgcaacc ccgtgggcat cggcaagaag taagcgccga ctcgagacca gagattcagg
    10501 agaaacaaat gaaaatccgc gcgcaattcg cgatggtgtt caacctcgac aagtgcatcg
    10561 gttgccacac ctgctcggtg acctgcaaga acgtgtggac caaccgcaag ggcgtcgagt
    10621 acgcctggtt caacaacgtc gagtccaagc cgggcatcgg ttacccgaag gaatgggaga
    10681 accaggacaa gtggaagggt ggctggacca agctcaacgg caagctcgag ctcaaggccg
    10741 gcggccgcct gaagaagatc gtgcagatct tctccaaccc gaacctgccc gagatcgacg
    10801 actactacga gcccttcacc tacgactacg cccgtctgca gaacgcgccg ctgtccgagg
    10861 ccgcgccgac cgcgcgtcca gtgtcgcaga tcaccggcga gaagatggac aagatcacct
    10921 ggggcccgaa ctgggaagac gacctcgccg gcgagttcgc ctcccgtgct cgtgacaaga
    10981 acttctccaa catggagaag gaaatgtaca agcagttcga aaacaccttc cacatgtacc
    11041 tgccgcgcat ctgcaaccac tgcatcaacc cggcttgcgt ggcctcgtgc ccgtccggcg
    11101 cgctctacaa gcgcgaggaa gacggcatcg tgctcgccga ccaggaccgc tgccgcggct
    11161 ggcggatgtg catctcgggc tgcccgtaca agaaggtgtt cttcaactgg gagtcgtcca
    11221 aggccgagaa gtgcgtgggc tgctacccgc gcgtcgagaa cggcatgccg accgtctgtt
    11281 cggagtcctg cgtcggccgc atccgctaca acggcatcat cctgtacgac gccgacaaga
    11341 tctcggccgc ggccagcgcc gacaacgtcc aggacctgta ccaggagcac ctggacatgt
    11401 tcgtcgatcc cttcgacccg gccgtgatcg agcaggccaa gaaggacggc atcaccgacg
    11461 actggatcct cgccgcgcag aagtcgccga tctacaagat ggccgtgcag tggaagatcg
    11521 ccttcccgct gcaccccgag taccgcacgc tgccgatgat ctggtacgtg ccgccgctct
    11581 cgccggtgca gtcgcagatc gaccagaagg cgctgccgac cgaggccgac ggcgttatcc
    11641 cgaaggcctc cgcgatgcgc ctgccggtca agtacctggc caacctgatg accgcgggca
    11701 aggagcagcc gatcatcgac gcgctcaacc gcatgatcgc gatgcgctcc taccagcgct
    11761 cggtgcacgt cgagggcacg cccgacacgc gcgcgctgga ggcggtgggc atgaccgagg
    11821 acatggccaa ggagatgtat cgctacctgg cgatcgcgaa ctacgaagac cgcttcgtga
    11881 ttccgacctc caaggaagag atgcgcctcg aggactacca cggcttccag ggtcaggcgg
    11941 gcttcagctt cggcagcgcg tcgtcggtcg gcatctcgcc gaactcgctg ttccccgagc
    12001 gccgcaagga gtcgatggag ccgcgcaagc cggtcccgca cgccgacgag ctgcacgacg
    12061 tcaccggcga acgctactga ggagactgaa ccatgcagac ctacaagctg cttgccatgc
    12121 tgctcgacta cccgggcagc gagatcgtcg gcgacctgcg cgccgcggtc tcggagcagg
    12181 gcggtgcgcg gggtctggtg cgctcggtgg acaccgacca ggtgttcacc gaggctgagc
    12241 acgggagcat cgccggtttc atcgactgga tgctcgctca ggaccagacc gagctcgaag
    12301 gccgtttcgt caagaccttc gacctcaccc cggagcacag cctgcacctg acccaccatg
    12361 tgttcggcga cgacaagaac cgcggtccgg cgctgatcga cctgtcggag ttctacaagt
    12421 cctacggact gcagcacgac gagcacgaga tcccggacta cctgccgatg atgatggagt
    12481 tcgtgtcgca actcggggag accgaggcgc gcgtgttcct gaccgacgcg gtgaaggtgt
    12541 tcaacgtgct cgcgaccaac ctggaaaagg cggagagccc ctatgcggcg ctcgttcgcg
    12601 tgatcgaaaa ccacagctcg ctggcccgcg cggccgcctg aggagaagac gatgtccctg
    12661 accaacctgt ttttcggggt ctatccctac attgcgctga ccgtgttcgt gatcggcagc
    12721 tggatccggt acgacaacga gcagtacacc tggaagaccg attcgtcgca gctgctgtcc
    12781 aagcaaggca tgatgctggc gagcaacctg ttccacgtcg gcgtcatcat gatcttcttc
    12841 ggccatttcg ccggtctcgt gctgccgcac ggcctgtggc ttggcctggg gatctccgac
    12901 ctcgcccacc aatggatcgc gatcatcgcc ggcgtgatct tcggcatcat gtgcctggtc
    12961 ggtggttcca tgctgtggta tcgccgcatg tacaacccgc gcgtgcgcat caacagccgt
    13021 cgcagcgacg tgttcatcat cacctggctg atggccacgc tgatcctcgg cctgctgacc
    13081 gtgccgttct cgatcggtca cgccgtcaag ggtgatccgg ccgtgatgct ggcgctggcc
    13141 gagtgggtca agagcatcct gctgctgaac ccgcagcccg agctgatgac gaaggtcgat
    13201 ccgatcttcc gtgcccacat cttcttcggc atgacggtgt tcctggtgtt ccccttcacc
    13261 cgcctggtgc acatcctgac cgcgccgttc agctacctga cgcgcgccta ccagatcgtc
    13321 cggaccaagc gccgcctggc ctgaggctga agcgagaggc agggctggca gccatcgagg
    13381 ctgccggcct tgcctttcgg tccttttgcc cctagcttgc gcggatgcgc cagaaagcac
    13441 cgattctcat gttcatccgc ggctcgggcc tgtcggtcaa gatcaccggc atgctgctcg
    13501 gctttttcct cgtggcactt gccgcgatcg cgctgacgct gttcatctcg tggcgcctgg
    13561 aaggcgcggc ggctgcgatc aacgatgccg gcagcctgcg gatgcgcttg taccgcgtgg
    13621 cgcatcacct cgctcgtgcg gatctgcaca ccagcgaccg gcagggtttc gctggcgacc
    13681 tgagggcgag ggtcgacgac ttcgaccgca tcctcggcgt gctgcgccgg ggagatccgt
    13741 cccgcccctt gttcgtgccg cgcgaagccg gtatccagca ggtgctctcc gggatgtcgg
    13801 aagaatggag cgtgcagctg cgcccgatcc tgctcgcgat cgccgcggac ccggatccgg
    13861 ccaacctgca tcgcaggggc ctggagttcg accgcgccgc ccgcgactac gtcgaggaca
    13921 tcaacgattt cgtgctgcgc atggagcaga gctatgcgcg caacaccaac atcctgcgca
    13981 cctcgcaggt gatcctggtt gccctcgcgg tgatcggcac catcctgctg ctgcgcttct
    14041 tcttcctcac cgtgatccgc cccgtgttcg agctccacga gggcgtcaag cgcatggggc
    14101 aggaggattt cgatgcccgc gtgccggtac tgtcgcagga cgagtttggc gagctctcgg
    14161 ccggcttcaa ccgcatggca gcccacttgc agaacgtcta tgacacgctc gaggagcgtg
    14221 tcgtcgccaa gacccgcagc ctcgaagaga agaaccgcga gctcgagatc ctctacgaga
    14281 tcgtcgcctt cctgcgcgaa cccaacgacg tcgacacgct ttgcaagggc ttcctcgagc
    14341 gggtgcagaa aacgatgggt gcgagtgcga gctccgcgcg cctgctcgat ctcgaatcgc
    14401 gaaacctgtg catgatcgtc cacgacgggc tgggcgacga cttcctcgat cgcgaggaag
    14461 tgctggcctg cggcgaatgc gtctgcggcg aggcggcaca gggcagcggg ctttcgctga
    14521 ccctggatgt acgcaatgcc gcgctcgcgt tgacgcgcga tgcctgtcgg cgcgccgggt
    14581 tccgtacggt gtcggccacc tcgattacgg ccagcaagcg cgcgctcggc gtgttcaacc
    14641 tgtatttcga ccagtcggat gcgatcggcg acgccgatcg ccagctgctc gaggccctcg
    14701 ggcagcagct cggcatcgcg atcgagaacc agcgcctgca cgcgcgcgag cgcgaactcg
    14761 ccgtctccga agagcgcaac ctgctcgcgc gcgaactgca cgactcgatc gcccagggcc
    14821 tggccttcct gaacctgcag gtgcagatgc tggaacgcgc gctcgccgag aaccgcgccg
    14881 cggacgtgag tacgaccgtg gagatgcttc gtcgcggtat ccaggagagc tatgaggatg
    14941 tgcgcgagtt gctggtgcac ttccgcaccc gggtcgagca gcaggatctc gatgccgcca
    15001 tcgccgcagc cctgcgccgg ctcgcagagc agaccggcat cgccaccgac ctcgacgtgc
    15061 agggcgacgg cgcaccgctc gatcccgagg ccgagaccca ggtgctctac atcatccagg
    15121 aagcgctctc caacgtgcgc aagcacgccg gtgcaagcac cgtttccgtg ggtgtgcggc
    15181 gaggtcgcga cgggctcgcg gtgacggtgc gcgacgacgg cgtgggcttt gcggaagacc
    15241 gtgacgcgtc cgacagcgag gcgcacatcg gcctgcagat catgcgcgag cgcgccatgc
    15301 ggatcggtgg ccgctttgcg gtgcgctcgt cgcccgggcg gggcaccgag atccgactgg
    15361 aactgccgcg aacgaacgag gtgctggctt gatggctgaa gagaagatcc gtgtccttct
    15421 ggtcgacgac cacacgctgt tccgcagcgg catccgttcg ctgctgcaga gctacgtctg
    15481 cttcgaggtg gtgggcgagg ccggcgacgg gcgcgagggc atccgcctcg ccgggcagct
    15541 gcgacccgac gtggtgttgc tcgacaacaa catgcccggg ctctccgggc gcgaggccgc
    15601 ccgcctgctg ctcgaggagg tgcccgagac gcgtgtgctg atgttgacgg tctcggagga
    15661 cgccgacgac cttatcgaga cgctgcgcgc cggggcctgc ggctacctgc tgaagaacat
    15721 ccaggccgaa accctggtat cggcgatcaa gacggccgcg cagggcgaat cggtggtttc
    15781 gccccagatg atgggcaagc tgctgagcgg cgtgcgcagc agcgccagcc gagcggcgat
    15841 gggcggtgcg atcgcgcctg cgccgggagc gctggcgagg agtgcccctg ctgccggtgc
    15901 ggagccggcc gaactcgaga agctgtcgcc gcgcgaacgc gagatcctgc gcttcatcgc
    15961 gcgcggacag agcaacaagg agatcgcccg tgcactcgag ctcgccgaga gcacagtgaa
    16021 gatccacgtg cagaacatgt tgcgcaagct caacctgagc agccgggtgc aggcggcggt
    16081 attcgcggtg gagaacggct tggggcaggg accggagggc tgagctccgc cagcgggctt
    16141 gcagcaggct tccgaaggct tcagcgcaat tgcgaggcga ggtcgcggaa cacaccgccg
    16201 agcagcttga cctcctcgcg gtggtgcagt gcgatcgttg ccgcgaggcg ctcgccttcg
    16261 gcggtcaggt gcacctcgac cgcgcgccga tcctcgcgac ccggacggcg ctcgaccagt
    16321 ccgagtttct cgcaacgcga aaccagcgac accactccat gctggtgggc ctgcaggcgc
    16381 tcggcgagct cgccgatgca ggcccattcg cgtccgggat agcccttgac ctggagcagc
    16441 agcaggtatt ggaggttggt gatgccgtgg cgccgggtga gcaactcgga gaagcgcagg
    16501 aagcgccgca gctggtagcg gaagtcggcg agggcctcga attcggactt gtcgagtgga
    16561 gtgttcatcg tgcgcggatt ttagcttctt ccgccctcgc ttcggcagtc cgatcgagag
    16621 atcgtccttg caatccgacg attgcggatt aatatcaaag tgtgatgtaa aaagagcgat
    16681 ggcccacgcg cgcaggtgga tcgtggcggc ggccggattt ccaccccgaa agaggagacc
    16741 gaatcatgga cgtcatgcaa tttccccgca tggtggtttc gagccgcgat ggctgggact
    16801 tggtcgcgcg cgaacggcca ccgatggact ggctgttccg tcgcctggtc ctgccgatgt
    16861 cgctgctgcc gccgctgatg atcctgctgg catcgggggg cgtgggcgcc cgggtgtttc
    16921 ccgacgcggg cttcggcaac tggttgatcg cggccgtgct gttcttcgtc gccgaacact
    16981 tcagcgtgcc gatcatggcg aactcgatcc gccaggcctc gatggccaag gacggcagca
    17041 gccggctcga ggatgcctac acggtggcgg cgatcgcgcc ggtgccactg tggctgtcct
    17101 cgctcgcggt ggtgttcggc agcgcgtggt tcgtggtgct cgtcggcctc gtcgcgctcg
    17161 gcgcctcggc catgctgatc cgccacggcg tcgaccgcct gctcggcatc gacgaggacg
    17221 tcgaggcgag cgaggtggcc atgcaggtga ccagcttcgg cgtgctgacc tggctggtgc
    17281 tgctggcggt cgcggggggg gcggtgacgc tgctgtgagg gcgctcaggc cttgacccgc
    17341 atgaggcgct gcttctcgcg ctcccagtcc ttcttcttct cgtcctcgcg cttgtcgtgc
    17401 agcttcttgc ccttggcgag cccgatctcg agcttcacga gcccattctt gtagtgcagg
    17461 tcgagcggca ccagcgtata gccggcgcgc tcgaccttgc cgatcaggcg ggcgatctcc
    17521 ttcttgtgca gcagcagttt gcgcgtgcgc gtcgggtcgt ggcgcacatg gctggaggcg
    17581 gtgctgagcg gggtgatgtg catgccgaaa acaaagatct cctcggcgcg gatcaccacg
    17641 taggattcct tgatgttcga gcgaccggcg cggatcgcct tgacctccca gccttcgagg
    17701 acgatgccgg cttcgtgctt ctcctcgatg aagtagtcgt gaagggcctt gcggttttcg
    17761 atgatgctca tggttcggcg tcccgtggcg gcgcggccct gagccggcgc gtccgtatgc
    17821 ggtagaatcg cggattttaa cagttcggcc gcacctccgt tccgtcatgg tggtggcgcc
    17881 gaccaacttg cccatggccg aagtcaacaa gctcgtcctg atcgaattca ccccggcgca
    17941 gatgttcgag ctcgtcgacc gctgcgagga atacccgctg tttctgccct ggtgcggcgg
    18001 cgtcgatctg atcgggcgca ccgagacgat caccgcggcg acgctgcaca tcaattacca
    18061 tggcatcaag gcgcatttca gcaccgagaa cgccaagcgc tacccgcagg agatgaagct
    18121 gcgcctgacc gacgggccct tcacccatct cgacggacac tggcgcttca ccgcgctcgg
    18181 cgagaccgcg tgcaaggtcg agttccagct gcgctaccag ttcgcgagca agctgctgga
    18241 gaaggtgctc gggccggtgt tcaaccatat cgcgaacact ttcgtcgatt ccttcgtcaa
    18301 gcgtgccggc caggtctatc gggccgggga ggggagtgct gcatgagcgg gatgatcaac
    18361 gtggaagtgg tgtacgcgct gccgcagcgc caggagctgg tgcacgtgcg gctggcggaa
    18421 ggcgcctcgg tgcgcgatgc gctggaggct tccggcctgt tgcagaagca tccggagatc
    18481 cagcccgacc gtgcgaacaa gctggggatc tatgccaggc tggtcaagct cgacaccgaa
    18541 gtgcgcgacc gcgaccgggt ggagatctat cgtccgctga tcgccgaccc gaaggcggtg
    18601 cgaaagaagc gtgccgacga gggcaaggtc atgaaaaagg ggggtgggcc cgccgaagcg
    18661 gccgagtgat ggatcggcgc gacccggcgc agcggaattt ccgctcgcgc gacgttcggc
    18721 ttgcgagcct gctggcgtcg acctgcgggt cggatctacc tgcaatgctc tgcgatcttg
    18781 cgctccagag cctcggcctg ctccgcgcgc gcacgatcgt cgaggaactc gcgttccccc
    18841 ttggcgttga agcggaccat gcgctggccg ccacgcaagg cggcgagttc atgctgcgcg
    18901 tccgtgcaga aacgttccgt ctcgcctctc gattccgctt cccgagcggc cttcgcctcg
    18961 gcctccgccg cggcggcgcg gcgttcccgg aaagcctgtt cgcgctcggc gagcgaactc
    19021 ggggaggtgg gagtggcagc aggcggcatg tctgccgacg gcgcggtctc tccagtcttg
    19081 cgcgcggcgg cacccggtcg tgctccgcgg atcaccgtgg cctcgacgcc tgcgggaggc
    19141 atgtcgccgt agtggatgcg cccgtccttg tccttccagc tgtagacctg cgcggccgcg
    19201 ggaagggcgg tcagcaacag ggcgaggatc gcgaccgggg cgaacggatg ggtcatcggc
    19261 acaattctcc gcggcgagcc gcttacgtgg tgcaggagcg ctctgtataa tacggatttg
    19321 gtcgaaagga aaaaagccat gcgagtgatc cagaaggcgc tgaccttcga tgacgtcctt
    19381 ctcgttcccg cccactccac ggtcctccct cgcgacgtga gcctgcagag ccaggtcacg
    19441 cgccgcatcc gcctcaacgt cccgcttgtc tcggccgcca tggacacggt gaccgagagc
    19501 cgtctcgcga tcgcgctcgc gcaggaaggc gggatcggcg tggtgcacaa gaacctcacc
    19561 ccggcccagc aggccgccga ggtgctcaag gtcaagcgcc acgagtcggg catcctcaag
    19621 gatccgatca ccatcccgcc cacgatgacg gtgggcgagg tcatcgcact gcaacgccag
    19681 aaccgcttct ccggcgtgcc ggtggtcgaa ggcggcaagg tggtcggcat cgtcaccaac
    19741 cgcgataccc gcttcgagac caagctcgac cagctcgtct ccgaaatcat gaccccgcag
    19801 gaccgccttg tcaccgtgcg cgaaggcgcc agcctcgaag aggcgcgcga gctgctgcgt
    19861 gtgcaccgcc tcgagcgcgt gctggtgctc aacgatgccg gcgagctgtg cggtctcatc
    19921 accgtcaagg acatgatgaa gtccaccgag cacccgctcg ctgccaagga cgagcatggt
    19981 cgcttgcgcg tggccgctgc gatcggcgtg ggggccggca cggaggaacg cgcggagcgc
    20041 ctcgccgacg cgggcgtgga catgatcgtg gtcgacaccg cccacggtca ctcgcagggc
    20101 gtgctcgacc gcgtcggctg ggtcaagaag cactttccgc acatcgaggt ggtgggcggc
    20161 aatatcgcca ccgccgacgc cgcgcgtgcg ctggtcgatg ccggtgccga cggtgtcaag
    20221 gtcggcatcg gtccgggctc gatctgcacc acccgcatcg tcgccggcgt gggcgtgccg
    20281 cagatcagcg cgatcgacaa cgtcgccaac gcgctgctgg gcacgggcgt gccgatgatc
    20341 gccgacggcg gcatccgctt ctcgggcgac atcgccaagg cgatcgccgc gggcgccgac
    20401 gtggtcatgc tcggtggact gtttgccggc accgaggagg cgccaggcga gactgtgctg
    20461 ttccagggcc gttcctacaa gtcgtaccgc ggcatgggct cgctcggcgc gatggagaag
    20521 ggcgcggccg accgctactt ccaggaagag aacaccggca acatcgacaa gttggtgccc
    20581 gagggcatcg agggtcgcgt gccctacaag ggctcggtca cggcggtgat ccatcagctc
    20641 gtcggcggcc tgcgcgcctc gatgggctac ctcggctgcg ccgacatccc gaccatgcat
    20701 gcgcgcgcgg agttcgtgca gatcagctcg gccggcatgc gcgaatcgca tgtgcacgac
    20761 gtgcagatca ccaaggaagc gcccaactac cagatcagct gagcgcgctc cttcgagaca
    20821 tccgggcggc tgcgaggccg cccttccttt ttcgacgttc gagattcccg ccccatggcc
    20881 caccagaaga tcctcatcct cgatttcggc tcccaggtct cccagctcat cgcgcgccgc
    20941 gtgcgcgagc agcaggtgta ctgcgagctg catcccttcg acgtctcgga cgagttcgtg
    21001 cgcaacttcg gcgcgcaggg cgtgatcctc tccggcgggc ccaactcggt ttacgaggcc
    21061 gaggactggc gcgcaccgca ggcggtgttc gagcttggcg tgccggtgct gggcatctgt
    21121 tacggcatgc agacgatggc cagccagctc ggcggcaagg tcgagagctc ggccaagcgc
    21181 gagttcggct atgcggagat gcgcgcgcgc gggcactcga agctgttcgc cggcatcgag
    21241 gaccgcagca acgccgaagg ccacggcctg ctcgacgtgt ggatgagcca cggcgacaag
    21301 gtcaccgcgc tgcccccggg cttcaagatc atcggcagca acgaatcgac tccgatcgcg
    21361 gcgatggccg acgaggcgcg cggcttctac ggcgtgcagt tccaccccga ggtcacgcac
    21421 acgatcaagg gcaaggagat gatcgggcgc ttcgtgcacg acatctgcgg ctgcggccac
    21481 gactggaaca tgcccgatta catcgaggaa gcggtgcaga agatccgcga gcaggtgggt
    21541 gacgaggagg tcatcctcgg cctgtcgggc ggcgtggact cctcggtggc ggcggcgctg
    21601 atccaccgcg cgatcggcga ccagctcacc tgcgtgttcg tcgaccacgg cctgctgcgc
    21661 ctgaacgaag gc
//
LOCUS       DAOQNN010000011        21081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_883_length_21081_cov_11.199990, whole genome shotgun sequence.
ACCESSION   DAOQNN010000011 DAOQNN010000000
VERSION     DAOQNN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 21081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21081
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_883_length_21081_cov_11.199990"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PKV97_01645"
     CDS             <1..689
                     /locus_tag="PKV97_01645"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNB04605.1"
                     /translation="LLTANGAIFTVQGKAIAENAKEDVRVLVVGNPCNTNAYIARAAA
                     IKVGRTNPNNYHGMLRLDHNRALSQLAAKSGREVSSLKKMVVWGNHSPSMYADYRFCE
                     SNGDSVKALINDHAWNNDVFLPTVGKRGAAIIEARGLSSAASAANAAIDHVHDWVLGS
                     SDWVTMGVPSDGSYGIPEGVVFGFPCECKGGQFKIIQGLEIDEYSKGKIAITLKELED
                     ERAAVADMLK"
     gene            834..1832
                     /locus_tag="PKV97_01650"
     CDS             834..1832
                     /locus_tag="PKV97_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNB04606.1"
                     /translation="MSLHPAQVLFAGEKPFPALPAVDHYAGAEKLMMKALKLQQELGP
                     VFDLTCDCEDGAQAGAEAEHAQMVVEVVNGAENHFGRVGARIHDVTHPHWQRDLEILV
                     GGAGRRLAFVTIPKVRSAADAAEQIRGLRRVEAACGLERALPVHVLIETHGALREAWE
                     IAALDGVESIDFGLMDFVSGHHGAIPGNAMKSPGQFEHPLVVRAKADIAAAALANGVV
                     PSHNVTTELQDVAVIRNDAERARREFGFLRMWSIHPNQILPIIAAMQPDFSEVEEAAA
                     ILCAAQDNAWGPIRHQGRLHDRASYRYYWELLQRARNTGMELPGETRPRFFTGAGH"
     gene            complement(1813..2739)
                     /locus_tag="PKV97_01655"
     CDS             complement(1813..2739)
                     /locus_tag="PKV97_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB04607.1"
                     /translation="MELRHLRYFIAVAEELNFTRAATRLHIGQPPLSMQIRDLEEEIG
                     VRLFERGPRRVALSTAGERFLLHARRILDGVEEAVAEARRAARGELGELRVGFTSSLP
                     FTDLLPDALNAYRRRFPQVRLQLREMFTPEQFQALVRGELDVGFVRLQAGTPPAGIVL
                     REVARNPLRLVVNAAHRLAGAAQVHLAELAGEDFISFPADVGTDLPGVLRALCRQAGF
                     EPRIVQTAREATTQIGLVAAGLGVAVLPAPLESVRIARVRYLALAEPEAQFRLALAIR
                     EGEPVPTLGGFLEVLESLRAGEPPLALSVPRQ"
     gene            2934..4082
                     /locus_tag="PKV97_01660"
     CDS             2934..4082
                     /locus_tag="PKV97_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB04608.1"
                     /translation="MFVGGFATFAMVYSTQPLLPLLAAEFGVGAASASLTVSATTAGL
                     ALMLIPASVLADRLGRQQVMKAALVIAALFALATAFAPDFGSLLILRTLLGGVIAGLP
                     AAAMAYIGEEIAPGAQARAMGLYIAGNALGGMSGRFVAALLTDWSSWRVALGMIGVLG
                     AVSALVFWRRLPASRNFRARAATPARIFADTLAIYRDPGLPALFLVAFLAMGGFVGLY
                     NFLGFRLLEPPYGLGQSAIGAIFLLYLVGTWASAASGRLAERRGRRKVLWPMVAVMGA
                     GLALTLARPLWLIIAGVGVFTFGFFAAHALASGWVGRRAGERRALASALYLSSYYLGA
                     SVLGSLAGTAWADWRWAGVAALVGLCVLFTGACALWLRRLPAPAEGAD"
     gene            complement(4100..5008)
                     /locus_tag="PKV97_01665"
     CDS             complement(4100..5008)
                     /locus_tag="PKV97_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB04609.1"
                     /translation="MKRAQANGLLLVAALIWGTAFVAQQTGMRDVGPFTFTGARFLFG
                     AVMVAPLAVLELRRLGRRGTFLDARDLLACALLGAVLFLGAALQQIGIMSTTVSNAGF
                     LTALYVPLVPIAGALLLRERSHWSVWPASLGCVAGTYLLGGGSLAALSVGDLWVIASA
                     LFWAAHVMLVGRVAARKGASVTVAMLQFLVVGGLGMAVGFGTEEVSSGALERALPSIA
                     YAGILSVGVGFTLQVVAQRHTPAADAAIVLSCETLFAALAGRFVLGETLTAEQMAGGL
                     LIFACVLAVQILPLWRERRTVSVEAG"
     gene            5241..6173
                     /gene="rocF"
                     /locus_tag="PKV97_01670"
     CDS             5241..6173
                     /gene="rocF"
                     /locus_tag="PKV97_01670"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNB04610.1"
                     /translation="MENLKVSLIGVPTDVGAGVRGASMGPEALRVAGLGRAIAAFGAE
                     VRDCGNLAGPSNPELPAVNGFRHLAEVAQWNLAVHQAVHAELSEGRVPIMLGGDHCLA
                     IGSISAVARHCRETGRKLRVLWFDAHADLNTATMTPSGNIHGMPVACLCGHGPEMLTG
                     IGGEMPAIRPEQIRQIGIRSVDEGEKRFLHDLCVEVFDMRYIDEIGMRATMEQALAGV
                     DQDTHLHVSLDVDFLDPTIAPGVGTTVRGGPTYREAELCMEMIADTGQLASLDIVELN
                     PALDHCNMTAELAVDLVESLFGKSTLMRMHRNYR"
     gene            complement(6291..7073)
                     /locus_tag="PKV97_01675"
     CDS             complement(6291..7073)
                     /locus_tag="PKV97_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB04611.1"
                     /translation="MNHKASVEQVRVEDLHKSYGDHEVLKGVSLTANSGDVISIIGSS
                     GSGKSTWLRCINFLECPTAGRILVGGKEVELVRDRKGELVVKDAHQLQQIRTRLSMVF
                     QHFNLWSHMTVLENVVEAPVHVLGLSKAAARERAERYLERVGVWKFRDAYPAHMSGGQ
                     QQRVAIARALAMEPAVLLFDEPTSALDPELVGEVLKVMRSLAEEGRTMMVVTHEMGFA
                     REVSNHVIFVHQGRVEEEGDPKEVLVRPRSERLQQFLSGNLK"
     gene            complement(7186..7920)
                     /locus_tag="PKV97_01680"
     CDS             complement(7186..7920)
                     /locus_tag="PKV97_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB04612.1"
                     /translation="MEFFDFKVITGSLPEFWRGLWMTVQLTGLALLFGFFVAIPLAVM
                     RLSPNRLVSAPVAAYTYFFRGTPMLVQLLLVYYGASQWEWMRAAWEAGHPLWSLFREP
                     YFCALFAFGLNTCAYTVEILAGSMRNTPHGELEAAKAMGMTRLKALQRIVIPSALRRM
                     IPSYSNEVILMLQGSAIASAVTLVDLTGAARNVYSRHFAPFESFVFVGLIYLALTFAL
                     VGLFKLAEHRWLAHLAPRKAEAGRQS"
     gene            complement(7929..8621)
                     /locus_tag="PKV97_01685"
     CDS             complement(7929..8621)
                     /locus_tag="PKV97_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB04613.1"
                     /translation="MDALIEYSPSLLAGAWVTLKVALMSVLLALATGLIGAACKLSGF
                     LPLRLWTMLYTTVMRGVPDLVMMLLVFYGGQLLLNAVIDYFGWDFVEIDPFVAGVLTI
                     GLIFGAYFTETFRGAFLAVSAGQLEAGRAYGMSAWQVFRRILFPQMLRFALPGIGNNW
                     LVLLKSTAIVSMIGLSDMTRIADQAGRSTHQPFTFYLAVCVLYLAMTALSSYGLGVLT
                     RRYSAGVRETRV"
     gene            complement(8728..9519)
                     /locus_tag="PKV97_01690"
     CDS             complement(8728..9519)
                     /locus_tag="PKV97_01690"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB04614.1"
                     /translation="MKKYALLCAVLALHAGAGFAKDWNEIRLASEGAYPPFNLIAADG
                     SLQGFDIDIGNALCEEMKAKCTWVKQEWDGMIPALVSRKFDAIIASMSITDERKAKVD
                     FTEKYYASPLALIAKKGSTLRPDLPSLAGKKVGVQRGTVSDNFATKYWDGKGMQIIRY
                     AKQDEAYLDLRAGRLDAAFSDYLEAYGGFLTKPEGAGYDVAGERLFGKDADEKAVIGE
                     GIGIAVRKRDKELTEKLNQALGAIRANGKYDAIQKKYFPMDIYGN"
     gene            9823..10839
                     /locus_tag="PKV97_01695"
     CDS             9823..10839
                     /locus_tag="PKV97_01695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNB04615.1"
                     /translation="MPSSAPLSVLDLAPVAAGSTPADALRNTRELAQLADRCGYTRYW
                     LAEHHNMTGIASAATAVVIGHIAGATRRIRVGAGGIMLPNHAPLVIAEQFGTLESLFP
                     GRIDLGLGRAPGTDQTTARALRRNLAGSDDQFPHDVLELQSYFQPASPGQAVRAVPGE
                     GLQVPIWLLGSSLYGAELAAHLGLPFAFASHFAPRFLFQAVEIYRSNFRPSAQLDKPW
                     FAVAANAIAADTDADAERLLRSLRLRFAAMARGVRGQLQAPEAFIEADWSPAELAFAE
                     QSLACSAVGAPDTVRQQLEALIERTGANELILTAQIFDHGARLRSMELVAQAWGLAVE
                     EAAA"
     gene            complement(10976..13399)
                     /gene="lon"
                     /locus_tag="PKV97_01700"
     CDS             complement(10976..13399)
                     /gene="lon"
                     /locus_tag="PKV97_01700"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNB04616.1"
                     /translation="MSGPAALPNEAMELPLLPLRDVVVFPHMVIPLFVGRPKSIKALE
                     SAMEDGKSILLVAQKSAAKDEPAVEDLYDIGCIANILQMLKLPDGTIKVLVEGVQRAR
                     IERVEDIRSLFVASVRPVPVAEAPSHELEAMRRAIIAQFDQYVKLNKKIPPEILGSLA
                     GIEEAGRLADTIAAHLPLKLEQKQEVLEMFDTGARLEKLLGQLEGELDILQVEKRIRG
                     RVKRQMEKSQREYYLNEQVKAIQKELGEGEEGADLEEMDKKIKAAGMPKEALAKAEAE
                     FKKLRLMSPMSAEATVVRNYIDTLISLPWKKKSRVSKDLGEAEKVLDKDHYGLERVKE
                     RILEYLAVQQRVDKVKAPILCLVGPPGVGKTSLGQSIAKATNRKFIRMALGGVRDEAE
                     IRGHRRTYIGSMPGKILQNMSKVGVKNPLFLLDEVDKLGMDFRGDPSSALLEVLDPEQ
                     NHTFQDHYVEVDFDLSDVMFVATANTLNIPAALLDRMEVIRLSGYTEDEKINIAQRYL
                     LPKQMKNNGLKREELSVTEEAVRDIVRYYTREAGVRSLEREISKVCRKVVKQLVLKSR
                     TSKVVVNARNLDKFLGVRKYSFGMAEKENQIGQVTGLAWTEVGGELLTIEAVQLPGKG
                     KVVTTGKLGEVMQESIQAALSVVRRRARSLGIDPDFYQKSDLHIHLPEGAIPKDGPSA
                     GIAISTALVSVLTGIPVRCDVAMTGEITLRGEVLAIGGLKEKLLAAVRGGIRTALIPE
                     ENVKDLAEIPDNIKNAIEIVPVKWIDRVLELALERKPEPLPEEPAPAAPATPAAEGVE
                     GDQANLRPH"
     gene            complement(13507..14775)
                     /gene="clpX"
                     /locus_tag="PKV97_01705"
     CDS             complement(13507..14775)
                     /gene="clpX"
                     /locus_tag="PKV97_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNB04617.1"
                     /translation="MTDKKAGGDKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCN
                     DIIRDEVVGAEDGEGIKGTLPTPHEICSILDQYVIGQSLAKRSLAVAVYNHYKRLRHL
                     DARQDDVELAKSNILLIGPTGSGKTLLAQTLARLLDVPFVMADATTLTEAGYVGEDVE
                     NIVQKLLQKCDYDVDKAQQGIIYIDEIDKISRKADNPSITRDVSGEGVQQALLKLVEG
                     TVASIPPQGGRKHPNQDFIQVDTTNVLFICGGAFDGLEKVIRNRTEKVGIGFGAEVKS
                     KEGKNLTDTFRQVEPEDLIKFGLIPELIGRLPVVATLQELDEAALIQILIEPKNALVK
                     QYQKLFSMEGVELEIRPNALHAVARKAIKRKTGARGLRSILEVALLDIMYDLPTLDNV
                     AKVVVDESTIEDGGKPLLIYAEQQKVSGAN"
     gene            complement(14794..15432)
                     /gene="clpP"
                     /locus_tag="PKV97_01710"
     CDS             complement(14794..15432)
                     /gene="clpP"
                     /locus_tag="PKV97_01710"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNB04618.1"
                     /translation="MMQGTPQHSSDWNPVGLGLVPMVVEQSGRGERAYDIYSRLLKER
                     VVFLVGPVNDVTANLIVAQLLFLESENPDKDIYFYINSPGGSVTAGMAIYDTMQFIKP
                     NVSTLCIGQAASMGAFLLAAGEKGKRFILPNSRVMIHQPLGGFQGQASDIEIHAREIL
                     YLRSRLNDMLSKHTGQALERIEKDTDRDNFMSATAAVEYGLVDKVLTSRDDT"
     gene            complement(15453..16760)
                     /gene="tig"
                     /locus_tag="PKV97_01715"
     CDS             complement(15453..16760)
                     /gene="tig"
                     /locus_tag="PKV97_01715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNB04619.1"
                     /translation="MEQNQVTPSALERRIDISVSLADIEKEVDARLKRMARTVKMPGF
                     RPGKVPMKIVAQTHGGQARGEAVGAAVEKAFGEKVRADSLRVAGYPSIEPKDGAAEGT
                     LEFTAVFEVYPDVVLGEFAGRKIERPVLEVGDAEVDKTIEVLRKQRTSFKAVERAAAQ
                     GDRVVIDFTGRMGGEVFDGGQAQDFPFVVGAGSMLKDFEDAVVGLAAGEGKTFDMTFP
                     EDYHAANLAGKQVQFEITVKAVEEPVLPEVDADFAKSLGVADGDVAKLREEVKGNLER
                     EVKRRIQSRLKEQVMDALIEVAPIEVPKALVQAESNQLADNARRDLEMRGLKTKDIPV
                     DPSWFKDQAERRVKLGLIMAELVKTSELHAKPEQIRALVEEMAQSYEDPSELVRWYYA
                     QPERLAQAEAVVIEDNVVEWVCAKADTSDKDVAFDELMGNNAA"
     gene            complement(16996..17127)
                     /locus_tag="PKV97_01720"
     CDS             complement(16996..17127)
                     /locus_tag="PKV97_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04620.1"
                     /translation="MDMVVSSSGAMRRERGCRKVKQVRVVRLLGGARLAVTGYRVCT"
     gene            17120..19042
                     /locus_tag="PKV97_01725"
     CDS             17120..19042
                     /locus_tag="PKV97_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HNB04621.1"
                     /translation="MSIFNAYQARFEAAREEEMSLQDYLELCRTDRMAYATAAERMLA
                     AIGEPELVDTRLDPRLSRIFSNKILKLYPAFRDFYGMEEVIENIVSYFRHAAQGLEEK
                     KQILYLLGPVGGGKSSLAEKLKSLIERIPFYAIKGSPVNESPLGLFNAAEDAHILEDD
                     FGIPRRYLGGIMSPWAVKRLHEYGGDITRFRVVKLGPSVLRQIAVAKTEPGDENNQDI
                     SSLVGKIDIRKLEQYSQDDPDAYSYSGGLCLANRGLLEFVEMFKAPIKVLHPLLTATQ
                     EGNYKGTEGFGAIPFDGIVMAHSNESEWVAFKNNRNNEAFLDRIYTVKVPYCLRVSDE
                     VRIYEKLLVHSSLAEAPCAPDTLKMMAQFAVLSRLKEPENSSIFSKMRVYDGENLKDT
                     DPKAKSYQEYRDYAGVDEGMTGLSTRFAFKALSKIFNFDHREVAANPVHLMYVLEQQI
                     EAEQFPPETEARYLGYIKEYLAPRYAEFIGKEIQTAYLESYSEYGQNIFDRYVIYADF
                     WIQDQEFRDPNTGEILDRAALNEELEKIEKPAGISNPKDFRNEVVNFVLRARAKHEGR
                     NPSWTSYEKLRAVIEKKMFSNTEDLLPVISFNAKASADELKKHQDFVDRMIDKGYTEK
                     QVRLLCEWYLRVRKAS"
     gene            19119..20387
                     /locus_tag="PKV97_01730"
     CDS             19119..20387
                     /locus_tag="PKV97_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HNB04622.1"
                     /translation="MVRIIDRRFDSKNKSAVNRQRFMRRFKQQIRKAVSEAIHGRSIR
                     DLENGEQISIPARDLSEPSLHHGKGGIWEQVFPGNDQFSTGDRIKRPLGGAGDGAGKG
                     KASQDGEHEDDFVFQLSREEFLDLFFEDLELPRLIRTQLAKVTDYKTRRAGFTSDGVP
                     ANINIVRSMRGALGRRLALGSPWAARIRALQQELDEALARAGEDSEEVRDLREELAAL
                     RARIERIPFIDRFDLRYNNRVKEPRPTTQAVMFCVMDVSGSMDEERKSMAKRFFMLLY
                     LFLTRSYEHIEVVFIRHHTVAKEVDEDEFFHSRESGGTVVSSALELMRNILRERYANG
                     QWNVYGAQASDGDNWDNDSPVCGRLLGKEILPWCQYFAYVEITAGEPQNLWREYAKLE
                     AAHDNFAMQRIESPADIYPVFRELFKKTIA"
     gene            20384..>21081
                     /locus_tag="PKV97_01735"
     CDS             20384..>21081
                     /locus_tag="PKV97_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNB04623.1"
                     /translation="MKTPRPRTRGRAAKAPLPSGSEWTVEAIDRYNEEIARAAAHYKL
                     DTYPVQVEIITAEQMMDAYASVGMPVNYHHWSFGKHFLATEKGYKRGQMGLAYEIVIN
                     SNPCIAYLMEENTLTMQGLVIAHAAYGHNSFFKGNYLFRTWTNADAIIDYLLFARNYI
                     TQCEERHGEEAVELLLDSCHALMNLGVDRYKRPPRLSLAKEKLRQQEREEYLQSQVND
                     LWRTLPAHELRTDAR"
BASE COUNT         3501 a         7376 c         6802 g         3402 t
ORIGIN      
        1 acctgctgac cgccaacggc gccatcttca ccgtccaggg caaggccatc gccgagaacg
       61 ccaaggaaga cgtgcgtgtg ctggtcgtgg gcaacccctg caacaccaac gcctacattg
      121 cacgcgccgc tgccatcaag gtcggtcgca ccaacccgaa caactaccac ggcatgctgc
      181 gcctggacca caaccgcgcg ctgtcgcagc tcgccgccaa gtccggccgt gaagtgtcct
      241 cgctcaagaa gatggtcgtg tggggcaacc actcgccgtc gatgtacgcc gactaccgct
      301 tctgcgagtc gaacggcgac agcgtcaagg cgctgatcaa cgaccacgcc tggaacaacg
      361 acgtgttcct gcccaccgtc ggcaagcgcg gcgccgccat catcgaggcc cgtggcctgt
      421 cgtcggccgc ctcggccgcc aacgccgcca tcgaccacgt gcacgactgg gtactcggct
      481 cgagcgactg ggtcaccatg ggcgtcccgt ccgacggctc ctacggcatc cccgagggcg
      541 tcgtgttcgg cttcccctgc gagtgcaagg gcggccagtt caagatcatc cagggtctgg
      601 agatcgacga gtactccaag gggaagatcg ccatcaccct gaaggaactc gaggacgagc
      661 gcgcggccgt ggccgacatg ctcaagtaag acgcgattgc gggcggccgc gtgccgccct
      721 cgcgacaggc cacggggtag aatccgtggc ctttttcatt tccgcgccgc gccggagccc
      781 cgcgcactgc ccgccctatg gccgccgacc cccccctaac cgaggagcct gaaatgtcgc
      841 ttcaccccgc ccaagtcctg ttcgccggcg agaagccctt ccctgccctg cccgccgtcg
      901 accattacgc cggcgccgag aagctcatga tgaaggcgct caagctgcag caggagctcg
      961 gcccggtgtt cgacctcacc tgcgactgcg aggacggcgc ccaggctggc gccgaggccg
     1021 agcatgcgca gatggtggtc gaggtggtca atggcgcgga gaaccacttc ggccgcgtgg
     1081 gcgcccgcat ccacgacgtc acccatccgc actggcagcg tgacctcgag atcctcgtcg
     1141 gcggcgccgg ccggaggctg gccttcgtga cgatccccaa ggtgcgctcg gccgcggatg
     1201 ccgcggaaca gatccgcggg ctgcgccgcg tcgaggccgc ctgcggcctc gagcgcgccc
     1261 tgcccgtaca cgtgctcatc gagacccacg gcgcgctgcg cgaggcctgg gagatcgccg
     1321 cgctcgacgg cgtggagtcg atcgacttcg gcctcatgga cttcgtctcc ggccaccacg
     1381 gcgccattcc cggcaacgcc atgaagagcc ccggccagtt cgagcacccg ctggtggtgc
     1441 gcgccaaggc cgacatcgct gccgccgcgc tcgccaacgg cgtggtgccc tcgcacaacg
     1501 tcaccaccga gttgcaggac gtcgccgtga tccgcaacga cgccgagcgc gcccggcgcg
     1561 agttcggctt cctgcgcatg tggagcatcc accccaacca gatcctgccg atcatcgcgg
     1621 cgatgcagcc cgacttctcc gaggtcgagg aagccgcggc gatcctgtgc gccgcgcagg
     1681 acaatgcctg gggcccgatc cgccaccagg ggcgcctgca cgaccgcgcg tcctaccgct
     1741 actactggga gctgctgcag cgcgcgcgca acaccggcat ggagttgccc ggcgagaccc
     1801 gcccgcgctt cttcactggc gcgggacact gagcgcgaga ggcggctcgc ccgcgcgcag
     1861 gctctccagc acctccagga agccgccaag cgtgggcacc ggctcgccct cccggatcgc
     1921 cagcgcaaga cggaactgcg cctccggctc ggccagcgcc aggtagcgca cgcgcgcgat
     1981 ccgcaccgac tcgagcggcg ccggcaacac cgccacgccc agcccggcgg ccaccaggcc
     2041 gatctgggtc gtggcctcgc gcgcggtctg cacgatgcgc ggttcgaatc ccgcctgccg
     2101 gcacagcgca cgcagcacgc cgggcaagtc tgtgccgacg tcggccggga agctgatgaa
     2161 gtcctctccg gcgagttcgg caaggtgcac ttgcgcagcg cccgccagcc ggtgggcggc
     2221 attcacgacc aggcgcagcg gattgcgcgc gacctcgcgc agcacgatcc ccgccggcgg
     2281 agtccctgcc tgcaggcgca cgaagccgac gtcaagctct ccgcgtacca gggcctggaa
     2341 ctgctccggc gtgaacatct cccgcaactg caggcgcacc tgcgggaagc gccggcggta
     2401 ggcgttgagt gcatcgggga ggaggtccgt gaagggcagc gaggaggtga agccgacgcg
     2461 cagctcgccc agctccccgc gcgcggcacg gcgggcttcg gccacggcct cttccacccc
     2521 atcgaggatg cggcgcgcgt gcagcaggaa gcgctcccct gcagtgctca gcgcaaccct
     2581 gcgcgggccg cgctcgaaca ggcgcacgcc gatctcctcc tccaggtcgc ggatctgcat
     2641 cgacagcggc ggctggccga tgtgcaggcg tgtcgcggcg cgggtgaagt tgagttcctc
     2701 ggcgacggcg atgaaatagc gcaggtggcg cagctccatc attcatttcc atatatggat
     2761 caagtgctca gaatatattg gacgatgggt tcggcgccat cctaagatcg ctgcagatcc
     2821 gctcggccta tcgccaccgc tacgccatgc ccgcacactc cgccgctcgt cccggcccct
     2881 cgcccgacca gctcgaggcc ggtacgcccg gctaccgccg cgccaacctg gcgatgttcg
     2941 tcggcggctt cgcgaccttc gccatggtct atagcaccca gcccctgctg cccctgctcg
     3001 ctgccgaatt cggtgtgggt gcggcgagtg ccagcctgac ggtgtcggcc accactgccg
     3061 gactcgcgct gatgctgatt ccggccagcg tgctcgccga ccgcctcggc cgccagcagg
     3121 tcatgaaggc ggcgcttgtg atcgccgccc tgttcgcgct ggccaccgcc tttgcgcccg
     3181 acttcggctc gctgctgatc ttgcgcacac tcctcggcgg ggtcatcgcc gggctccccg
     3241 ccgccgccat ggcctacatc ggcgaggaga tcgcccccgg cgcgcaggca cgcgcgatgg
     3301 ggctctacat tgccggcaac gccctcggcg gcatgagcgg acgcttcgtc gccgcgctgc
     3361 tgaccgactg gagctcctgg cgggtcgcgc tcgggatgat cggcgtgctg ggggcggtct
     3421 ccgcactggt gttctggcgc cgcctgccgg cctcgcgcaa cttccgcgcg cgcgccgcca
     3481 cgccggcgcg gatcttcgcg gacactctgg cgatctaccg cgatcccggc ctgcccgcgc
     3541 tcttcctggt cgccttcctc gccatgggcg gcttcgtcgg gctgtacaac tttctcggct
     3601 tccgcctgct cgagccgccc tacgggctgg gccagagcgc gatcggcgcg atcttcctgc
     3661 tctacctggt cggcacctgg gcctcggcgg cgagcggccg cctcgccgag cgccgcggcc
     3721 ggcgcaaggt gctgtggccg atggtcgcgg tgatgggcgc cgggctcgcg ctcaccctgg
     3781 cgcggccgct gtggctgatc atcgccggcg tcggcgtgtt caccttcggc ttcttcgcgg
     3841 cgcacgccct cgccagcggc tgggtgggcc ggcgcgccgg cgaacggcgc gcactcgcct
     3901 cggcccttta tctgtcgagc tactacctcg gcgccagcgt gctcggcagc cttgccggca
     3961 cggcctgggc cgattggcga tgggcgggcg tggccgcgct ggtggggctg tgcgtgctgt
     4021 tcaccggagc ttgcgcgctg tggctgcggc gactgccggc gcccgcggaa ggtgcggact
     4081 gaaggacgcg caggccgcct cagcccgcct ccaccgacac ggtccgtcgt tcccgccaca
     4141 aaggcaggat ctgcaccgcc agcacgcagg cgaagatcag caggccgccg gccatctgct
     4201 cggcagtgag cgtctcaccc agcacgaagc ggccggccag cgcggcgaac agggtctcgc
     4261 agctcagcac gatggcggcg tcggccgccg gggtgtggcg ttgcgccacc acctgcaggg
     4321 tgaagcccac gcccaccgac aggatgccgg cgtaggcgat cgacggcagc gcacgctcga
     4381 gcgcgcctga cgagacctcc tctgtgccga agcctaccgc catgccgagc cccccgacga
     4441 cgaggaactg cagcatcgcc accgtcaccg aggccccctt gcgcgccgcc acgcgcccca
     4501 ccagcatcac gtgggcggcc caaaacagcg cgctcgcgat cacccacaag tcgcccaccg
     4561 agagcgcggc caggctgccg ccgccaagca ggtaggtgcc cgcgacgcag cccaggctcg
     4621 ccggccacac cgaccaatgc gagcgctcgc gcagcagcag cgcgccggcg atcggcacga
     4681 gggggacgta gagcgcggtg aggaaaccgg cattgctcac cgtggtgctc atgatgccga
     4741 tctgctgaag cgccgcgccg aggaacagca ccgcgccgag cagcgcacaa gcaagcaggt
     4801 cgcgcgcgtc gaggaaggtg ccacgacgac cgaggcgtcg cagttcgagg acggcgagcg
     4861 gcgccaccat caccgcaccg aagaggaagc gcgcgccggt gaacgtgaac ggcccgacgt
     4921 cgcgcatgcc ggtctgttgc gcgacgaagg cggtgcccca gatgagcgcc gcgacgagca
     4981 gcaagccgtt ggcctgcgcg cgcttcatct cgtcgcctcg acggcgggcc gggtgaaaac
     5041 acgggaaaga cgaattgaaa cagccgacac ggatactccc aagggcacgg gccccatcga
     5101 tcgagagccg gggcaccgcc gcgcgcgcgg cagatcaccc ggcccggcaa aaaaggcgca
     5161 agtgttacac gctcgggggc gcccccgtca atcccgaccg cagctgccat cgcggcaaca
     5221 cctgaggtaa tctcgcggcc atggaaaatc tcaaagtcag tctgatcggg gtgcccaccg
     5281 acgtcggcgc aggcgtgcgc ggcgcgagca tgggaccgga ggccttgcgc gtagccggcc
     5341 tggggcgggc catcgccgcc ttcggcgccg aggtgcgcga ctgcggcaac ctcgccggcc
     5401 cgtccaaccc ggagctgccg gcggtaaacg gcttccgcca ccttgccgaa gtggcgcagt
     5461 ggaacctcgc ggtccatcag gccgttcacg cggaactgtc cgaagggcgc gtaccgatca
     5521 tgctcggcgg cgatcattgc ctcgcgatcg gctcgatcag cgcggtggcc cgccactgcc
     5581 gcgagaccgg gcgcaagctg cgcgtgctgt ggttcgacgc ccatgccgac ctcaacaccg
     5641 ccaccatgac gccgtcgggc aacatccatg gcatgccggt cgcctgcctg tgcggccatg
     5701 gccccgagat gctgaccggc attggcggcg agatgccggc gatccggccc gagcagatcc
     5761 gccagatcgg catccgcagc gtggacgagg gcgagaagcg ctttctgcac gatctctgcg
     5821 tcgaagtctt cgacatgcgc tacatcgatg agatcggcat gcgcgcgacc atggagcagg
     5881 cgctcgccgg cgtggaccaa gacacgcacc tccacgtgag cctggacgtg gacttcctcg
     5941 acccgacgat cgccccgggc gtcggcacca cggtgcgcgg cggaccgacc taccgcgagg
     6001 ccgagctgtg catggagatg atcgccgaca ccggccagct cgcctcgctc gacatcgtgg
     6061 agctcaaccc ggcgctcgac cactgcaaca tgaccgccga gctggcggtc gatctggtcg
     6121 agagcctgtt cggcaagagc acgctcatgc gcatgcaccg caactaccgc tgaacgcagc
     6181 gggacacccg ccggcgcggc ttgtcacggt gcttgtgcgc gacggccttc aaggcgtcgg
     6241 ccggagagcg tgggctgccg tgccgtaaca cgatcggcgc accggtccga tcacttcaga
     6301 ttgcccgaga ggaactgctg caggcgttcg ctgcgcgggc gcaccagcac ctccttgggg
     6361 tcgccctcct cctcgacgcg cccctggtgc acgaagatca cgtggttgga cacctcgcgc
     6421 gcgaagccca tctcgtgcgt caccaccatc atcgtgcggc cttcctcggc gagcgagcgc
     6481 atcaccttga gcacctcgcc gacgagttcg gggtcgagcg ccgaggtcgg ctcgtcgaac
     6541 agcagcaccg caggctccat cgccagcgcg cgcgcgatcg ccacgcgctg ctgctggccg
     6601 cccgacatgt gcgcaggata ggcatcgcgg aacttccaca cccccacccg ctccaggtag
     6661 cgctcggcgc gctcgcgggc cgcggccttc gacagtccga gcacatgcac cggcgcctcg
     6721 acgacgttct cgagcaccgt catgtggctc cacagattga agtgctggaa caccatcgac
     6781 aagcgcgtgc ggatctgctg caactggtgc gcgtccttga ccaccagctc gcccttgcgg
     6841 tcacgcacca gctcgacctc cttgccgccc accaggatgc gcccggcggt cgggcactcg
     6901 aggaagttga tgcagcgcag ccaggtgctc ttgccggagc cggaggaccc gatgatgctg
     6961 atcacgtcgc ccgagttcgc ggtcagcgag acgcccttga gcacctcatg gtcgccgtag
     7021 gatttgtgca agtcttcgac gcggacctgt tcgacgctgg ccttgtggtt catgttgttc
     7081 tcttgcatcg gggcttgcgc ggcacccggc atgtcgccac gagccgcggc gaaccgccgc
     7141 caggaatcgg cagcgttcgc cgcttcgggc tcgcggcagg gttgatcagg actgcctgcc
     7201 cgcctccgcc ttgcgcggag ccaggtgggc gagccagcgg tgctcggcga gcttgaacag
     7261 ccccaccagg gcgaaggtga gcgcgaggta gatcaggccg acgaagacga aggactcgaa
     7321 gggtgcgaag tggcgcgaat acacgttgcg cgccgcaccg gtgaggtcca ccagggttac
     7381 cgcgctggcg atcgccgacc cctgcagcat caggatcacc tcgttgctgt agctcgggat
     7441 catgcgccgt agcgccgacg ggatcacgat gcgctgcagg gccttcaagc gcgtcatgcc
     7501 catcgccttg gcggcctcga gctccccgtg cggcgtgttg cgcatgctgc cggcgaggat
     7561 ctcgacggtg taggcacagg tgttgaggcc gaaggcgaac agcgcgcaga agtagggttc
     7621 gcggaacagc gaccacagcg gatgcccggc ctcccacgcc gcgcgcatcc attcccactg
     7681 cgaggcgccg tagtacacca gcagcagctg cacgagcatc ggcgtgccgc ggaagaagta
     7741 ggtgtaggcc gcgaccgggg ccgacaccag acggttgggc gacagccgca tgaccgcgag
     7801 cgggatcgcc acgaagaaac cgaacagcaa cgccaagccg gtgagctgga cggtcatcca
     7861 cagcccgcgc cagaactcgg gcaagctgcc ggtgatgact ttgaagtcga agaattccat
     7921 gtccgccctc agacccgggt ctcgcgcacg ccggcactgt agcgccgtgt gagcacgccg
     7981 aggccgtagc tcgacagcgc ggtcatcgcc aggtagagga cgcagaccgc aagatagaag
     8041 gtgaatggct ggtgcgtcga acgcccggcc tggtcggcga tccgcgtcat gtcggagagg
     8101 ccgatcatgg acacgatcgc cgtgctcttg agcagcacca gccagttgtt gccgatgccc
     8161 ggcagcgcga agcgcagcat ctgcgggaac aggatgcggc ggaacacctg ccaggcgctc
     8221 atgccgtagg cgcgcccggc ttcgagttgg cccgccgaga cggccaggaa ggcgccacgg
     8281 aaggtctcgg tgaaataggc gccgaagatc agtccgatcg tcagcacgcc ggcaacgaag
     8341 gggtcgatct cgacgaagtc ccagccgaag tagtcgatca ccgcattgag cagcaactgc
     8401 ccgccgtaga acacgaggag catcatcacc agatcgggca cgccgcgcat caccgtcgtg
     8461 tacagcatcg tccacagccg cagcggcagg aaacccgaca gtttgcaggc agccccgatc
     8521 aggccggtcg ccagcgcgag cagcaccgac atcaaggcca ccttcagggt gacccaggcg
     8581 ccggcgagca ggctggggga gtattcgatc agggcatcca tggcgcaccg attcgcaaag
     8641 gtaaacgcgc acgctcccgg tccagggagc gtgcgcgcgg cagggtaacc gcaggggccg
     8701 cgctggcttg accgcagccc ggcatggtca gttgccgtag atgtccatcg ggaagtactt
     8761 cttctggatc gcatcgtact tgccgttggc gcggatcgcg ccgagtgcct ggttcagctt
     8821 ctcggtgagc tccttgtcgc gcttgcgcac cgcgatgccg atgccctcgc cgatcaccgc
     8881 cttctcgtcg gcgtccttgc cgaagaggcg ttcgccggcg acgtcgtagc ccgcgccctc
     8941 gggtttggtc aggaaaccgc cgtaggcctc gaggtaatcg gagaaggccg catcgagacg
     9001 tccggcgcgc aggtcgaggt aggcctcgtc ctgcttggcg tagcggatga tctgcatgcc
     9061 cttgccgtcc caatacttgg tggcgaagtt gtccgatacc gtgccgcgct gcacgccgac
     9121 cttcttcccg gccagggagg ggagatcggg ccgcagggtc gagcccttct tcgcgatcag
     9181 ggccagcggc gacgcatagt acttctcggt gaaatccacc ttggccttgc gctcgtcggt
     9241 gatcgacatc gaggcgatga tcgcatcgaa cttgcgcgag accagcgccg ggatcatgcc
     9301 gtcccactcc tgcttcaccc aggtgcactt ggccttcatc tcctcgcaca gggcgttgcc
     9361 gatgtcgata tcgaaacctt gcaggcttcc gtcggcggcg atgaggttga agggagggta
     9421 ggcgccctcg gacgccaggc ggatctcgtt ccagtccttg gcaaaaccgg cgccggcgtg
     9481 aagggcgagc accgcgcaca gaagggcgta ttttttcatc cagaactcct cgattgtctt
     9541 gttgtcggtt cttccgcgcc gctgcggcgg agcctcacgc acagcgacag atcgaccggc
     9601 agtgcagagg gggcgaatgg tgtcaaaaaa cgccctccac ctcaattgcc gtattccacg
     9661 atctgtgccc gttccggggt cctcggggcg ccacgatcga caccccgagc aagtttccgg
     9721 caagcgtatc acgccacgca atccgactgg cgctcgcccc cgggctggca ccggcggcgg
     9781 ctctatggcg taatggcgga cctcgttcca accgactttt ccatgccctc ctccgccccc
     9841 ctctcggtgc tcgacctcgc accggtcgcc gccggcagca cgccggccga tgcgctgcgc
     9901 aacacgcgcg aactcgccca gctcgccgac cgctgcggct acacccgcta ctggctggcg
     9961 gaacaccaca acatgaccgg tatcgccagc gccgccaccg cggtggtgat cggccacatc
    10021 gccggcgcca cccggcgcat ccgcgtcggt gccggcggca tcatgctgcc caaccacgca
    10081 ccgctggtta tcgccgaaca gttcggcacc ctggagtcgc tctttccggg ccgcatcgac
    10141 ctcggtctcg ggcgtgcgcc gggcaccgac cagaccacgg cgcgcgcgct gcgccgcaat
    10201 ctcgccggca gcgacgacca gttcccgcac gacgtgctcg agctgcagtc gtacttccag
    10261 cccgccagcc ccggacaggc cgtgcgcgcg gtgcccggcg aagggctgca ggtgccgatc
    10321 tggctgctcg gctctagcct ctacggtgcc gagctcgccg cccacctcgg cctgcctttc
    10381 gccttcgcct cgcacttcgc accgcgcttc ctgttccagg cggtcgagat ctatcggagc
    10441 aatttccgtc cgtccgccca gctcgacaag ccttggttcg cagtcgccgc caacgcgatc
    10501 gccgccgaca ccgacgccga tgcggagcgc ctcttgcgct cgctacggct gcgcttcgcg
    10561 gcgatggcgc gcggcgtgcg cgggcagttg caggcgccgg aagccttcat cgaggcggac
    10621 tggtcaccgg ccgagctcgc cttcgcggag cagtcgctcg cgtgctcggc ggtgggtgcg
    10681 cccgacacgg ttcgccagca gctcgaggcg ctgatcgagc gtaccggcgc gaacgaactc
    10741 atcctcaccg cacagatctt cgatcatggc gcgaggctgc gctcgatgga actcgtggca
    10801 caggcctggg gcttggcggt ggaggaggcc gcggcctgag cgcacacctg ctgggcggcc
    10861 accgacggag gtcgaaggcc gtgcaggtgc gacgcaaagc aataaagccg cgggcccttg
    10921 cggggcgcca cggcttattg gatccggcac cggcccgagg gccgggacgg caggtttagt
    10981 gcggacgcag gttggcctgg tcgccttcga caccttcggc cgccggcgtg gcgggcgccg
    11041 ccggggccgg ctcctcgggc agcggctcgg gcttgcgctc gagggcgagt tcgagcacgc
    11101 ggtcgatcca cttcaccggc acgatctcga tcgcgttctt gatgttgtcg gggatctcgg
    11161 cgagatcctt gacgttctcc tccggaatca gcgcggtgcg gatgccacca cggacggcgg
    11221 cgagcagctt ctccttgagg ccgccgattg ccagcacctc gccgcgtagc gtgatctcgc
    11281 cggtcatcgc cacgtcgcaa cgcaccggga tgccggtgag caccgacacc agcgcggtcg
    11341 aaatcgcgat acccgccgac ggaccgtcct tggggatcgc gccctccggc aggtggatgt
    11401 gcagatcgct cttctggtag aagtccgggt cgataccgag cgaacgcgca cgccggcgca
    11461 ccaccgacag cgcggcctgg atggactcct gcatcacctc gccgagcttg ccggtggtca
    11521 cgaccttgcc cttgccgggg agctgcaccg cctcgatggt cagcagttcg ccacccacct
    11581 cggtccatgc gagaccggtg acctggccga tctggttctc cttctccgcc atgccgaagc
    11641 tgtacttgcg cacgccgagg aacttgtcga ggttacgggc gttgaccacc accttgctcg
    11701 tgcggctctt gagcaccagc tgcttgacca ccttgcggca gaccttggag atctcgcgct
    11761 ccaggctgcg cacgccggcc tcgcgggtgt agtaacgcac gatgtcgcgc accgcctcct
    11821 cggtgaccga gagctcctcg cgcttgaggc cgttgttctt catctgcttg ggcagaaggt
    11881 aacgctgggc gatgttgatc ttctcgtcct cggtgtaacc cgacagacgg atcacctcca
    11941 tgcggtcgag cagcgcagcc gggatgttga gggtgttggc ggtggccacg aacatcacgt
    12001 ccgacaggtc gaaatcgacc tcgacgtagt ggtcctggaa ggtgtggttc tgctccgggt
    12061 cgagcacctc gagcagcgcg gacgacggat cgccacggaa atccatgccg agcttgtcga
    12121 cctcgtcgag caggaacagc gggttcttga ccccgacctt gctcatgttc tgcaggatct
    12181 tgcccggcat cgagccgata taggtgcggc ggtggccgcg gatctcggcc tcgtcgcgca
    12241 cgccacccag tgccatgcgg atgaacttcc gattggtggc cttggcgatc gactggccga
    12301 gcgaggtctt gcccacgccg ggggggccga ccaggcacag gatcggcgcc ttgaccttgt
    12361 ccacacgctg ttgcacggca aggtactcga ggatgcgttc cttgacgcgc tcgaggccgt
    12421 agtggtcctt gtcgagcacc ttctcggcct cgccgaggtc cttgctgacg cgcgacttct
    12481 tcttccacgg caggctgatc agggtgtcga tgtagttgcg caccacggtg gcctcggccg
    12541 acatcggcga catcaggcgc agcttcttga actcggcctc ggccttggcg agcgcctcct
    12601 tgggcatgcc ggcggccttg atcttcttgt ccatctcctc gagatcggca ccctcctcgc
    12661 cctccccgag ctccttctgg atggccttga cttgctcgtt gaggtagtac tcgcgctggc
    12721 tcttctccat ctggcgcttg acgcggccgc ggatgcgctt ttcgacctgc aggatgtcga
    12781 gctcgccctc gagctggccg agcagcttct cgaggcgtgc gccggtgtcg aacatctcga
    12841 ggacttcctg cttctgctcg agcttgagcg gcaggtgggc ggcgatggtg tcggcgaggc
    12901 gcccggcctc ctcgatgccc gcgagcgagc cgaggatctc gggcgggatc ttcttgttga
    12961 gcttaacgta ctggtcgaac tgggcgatga tcgcgcgccg catggcctcc agttcgtggc
    13021 tcggcgcctc ggcgaccggc accgggcgca cgctggcgac gaacaggctg cggatgtcct
    13081 cgacacgctc gatgcgcgcg cgctgcacgc cctcgaccag caccttgatc gtaccgtcgg
    13141 gcagcttgag catctgcagg atgttcgcga tgcagccgat atcgtagaga tcctcgaccg
    13201 cgggctcgtc cttggcggcg gacttctgcg ccaccagcag gatgctcttg ccgtcttcca
    13261 tggcgctctc gagcgccttg atgctcttcg ggcggccgac gaagagcggg atcaccatgt
    13321 gcgggaagac caccacgtcg cgcaggggca acagcggcag ttccatcgct tcgttgggga
    13381 gggcagcggg acccgacatt ttctaggttt cctcaagcaa aaggtcacac ccatatgagg
    13441 gcgtgacccg gaaattcaag cggccggccg gagccggcct cgccggatcg ggcgacggta
    13501 gcgcgctcag ttggcgcccg acaccttctg ctgctcggcg tagatcagca agggcttgcc
    13561 gccgtcttcg atggtcgatt cgtccaccac caccttggcc acgttgtcga gcgtcggcag
    13621 gtcgtacatg atgtccagca gcgccacctc gaggatggag cgcaggccgc gcgccccggt
    13681 cttgcgcttg atcgccttgc gcgccacggc atgcagcgcg ttgggacgga tctcgagttc
    13741 gaccccctcc atggagaaga gcttctggta ttgcttgacc agcgcgttct tgggctcgat
    13801 gaggatctgg atcagcgccg cctcgtcgag ttcctgcagc gtggcgacca ccggaaggcg
    13861 gccgatcagc tcggggatga ggccgaactt gatcaggtcc tccggctcca cctgacggaa
    13921 ggtgtcggtg aggttcttgc cttccttgct cttgacctcg gcgccgaagc cgatgccgac
    13981 cttctcggtg cggttgcgga tcaccttttc caggccgtcg aaggcgccac cgcagatgaa
    14041 cagcacattg gtggtgtcga cctggatgaa gtcctggttc ggatgcttgc gcccgccctg
    14101 cggcggaatc gacgcaaccg tgccttcgac cagcttgagc agcgcctgct gcacgccctc
    14161 gcccgacacg tcgcgggtga tcgaggggtt gtcggccttg cgcgagatct tgtcgatctc
    14221 gtcgatgtag atgatgccct gctgcgcctt gtcgacgtcg tagtcgcact tctgcagcag
    14281 cttctggacg atgttctcga cgtcctcgcc gacgtagccg gcctcggtca gcgtggtggc
    14341 gtcggccatc acgaaaggca cgtcgagcag gcgcgcgagc gtctgcgcga gcagggtctt
    14401 gcccgagccg gtggggccga tcagcaggat gttgctcttg gcgagttcga catcgtcctg
    14461 gcgcgcatcg aggtggcgca ggcgcttgta gtggttatac accgcgacgg cgagactgcg
    14521 cttggccagc gactgcccga tcacgtactg gtcgaggatg gagcagatct cgtgcggggt
    14581 gggcagtgtg cccttgatcc cctcgccatc ctccgcgccg acgacctcgt cgcgaatgat
    14641 gtcgttgcag agctcgatgc actcgtcgca gatgaagacc gaagggccgg cgatcagctt
    14701 gcgcacctcg tgctggctct tgccgcagaa ggagcagtac agcagcttgt cgccgccagc
    14761 cttcttgtcc gtcatgtggg ggttctccag aaatcaggtg tcgtcgcggc tggtgagcac
    14821 cttgtcgacc aggccgtact cgacggcagc cgtcgccgac atgaagttgt cgcgatcggt
    14881 gtccttctcg atgcgctcga gcgcctgtcc ggtgtgcttg gacagcatgt cgttgaggcg
    14941 cgagcgcagg tagaggatct cgcgggcgtg gatctcgatg tccgaggcct ggccctggaa
    15001 gccgccgagc ggctgatgga tcatcacgcg cgagttcggc aggatgaagc gcttgccctt
    15061 ctcgcccgcc gccagcagga aggcgcccat gctcgccgcc tggccgatgc acagcgtgct
    15121 gacgttcggc ttgatgaact gcatggtgtc gtagatcgcc atgccggcgg tcaccgaccc
    15181 gccgggcgag ttgatgtaga agtagatgtc cttgtccggg ttctcggact cgaggaacag
    15241 caactgggcg acgatcaggt tggcggtgac gtcgttgacc gggccgacga ggaacaccac
    15301 ccgctccttc agtaggcgcg aatagatgtc gtaggcgcgt tcgccgcgac cactctgttc
    15361 gaccaccatc ggaaccaggc cgagaccgac cgggttccag tcgctgctgt gctggggtgt
    15421 tccttgcatc attcgtcaga ccctctgcga cctcaggccg cgttgttgcc catcagttcg
    15481 tcgaacgcca cgtccttgtc ggaagtgtcg gccttcgcgc acacccactc gacgacgttg
    15541 tcttcgatga ccaccgcctc ggcctgcgcc agacgttccg gttgagcgta ataccagcgc
    15601 acgagttccg aaggatcctc gtaactttgc gccatttcct cgaccagggc gcggatctgc
    15661 tcgggcttgg cgtgcagctc gctggtcttg accagctcgg ccatgatcag gcccagcttg
    15721 acgcggcgct cggcctggtc cttgaaccac gacggatcga ccgggatgtc cttggtcttg
    15781 aggccgcgca tctcgaggtc gcgacgggcg ttgtcggcga gctggttcga ctcggcctgc
    15841 accagcgcct tgggcacctc gatcggggcg acctcgatca gcgcgtccat cacctgctcc
    15901 ttgaggcggg actggatgcg gcgcttgacc tcgcgctcga ggttgccctt gacctcctcg
    15961 cgcagcttgg cgacgtcgcc gtcggccacg cccagcgact tggcgaagtc ggcgtccacc
    16021 tcgggcagca ccggctcctc gacggccttg acggtgatct cgaactgaac ctgcttgcct
    16081 gccagattgg cggcgtgata gtcctcgggg aaggtcatgt cgaaggtctt gccctcgccg
    16141 gccgcgaggc cgacgacggc atcctcgaag tccttgagca tggagccggc gccgaccacg
    16201 aaggggaagt cctgcgcctg gccgccatcg aacacctcgc cacccatgcg gccggtgaag
    16261 tcgatcacca cgcggtcgcc ctgcgcggcg gcgcgctcga ccgccttgaa gctggtgcgc
    16321 tgcttgcgca ggacctcgat ggtcttgtcg acctcggcgt cacccacctc gagcaccggg
    16381 cgctcgatct tgcgtccggc gaactcgccc aggaccacgt cggggtacac ctcgaacacg
    16441 gcggtgaatt ccagcgtccc ctcggcggcg ccgtccttcg gctcgatgct cggatagccg
    16501 gcgacgcgca ggctgtcggc acgcaccttc tccccgaaag ccttctcgac cgccgcaccg
    16561 acggcttcgc cgcgggcctg gccgccgtgg gtctgggcga cgatcttcat cggcaccttg
    16621 cccgggcgga accccggcat cttcaccgtg cgcgccatgc gcttgaggcg ggcgtccacc
    16681 tctttctcga tgtccgcgag cgaaacggag atgtcgatac ggcgctcgag cgcgctcggg
    16741 gttacctggt tctgctccat tgagtcgatc ctgagaagtc tgaaaaacgc tcccgccggg
    16801 cgcttgcggc acactcgcgg ggaccggagg cgcactttgc cagcgtgagc acattgaact
    16861 gagtcgggca ttctagcaca ggcgtcgcgc ttgtcccgtg cccggaaaag gccctgtagc
    16921 gggtcgtgac acagtccacg cccgctcacg aaaaaagcgc cgcaaggggt ggaactccca
    16981 cccatcatgg ggttctcatg tacagacccg gtaccccgtc acggcgagcc gggcaccgcc
    17041 gagcaggcgg acgacgcgaa cctgcttcac cttgcggcag cccctttcgc gccgcattgc
    17101 gcccgacgag gagacgacca tgtccatctt caacgcctac caggcacgct tcgaagcggc
    17161 ccgcgaggaa gagatgtccc tgcaggacta tctcgaactc tgccgcaccg accgcatggc
    17221 ctacgccacc gccgccgagc gcatgctcgc ggccatcggc gaacccgaac tggtcgacac
    17281 ccggctcgat cctcgcctgt cgcggatctt ctccaacaag atcctcaagc tctatccggc
    17341 cttccgcgac ttctacggca tggaagaggt catcgagaac atcgtgtcct acttccgcca
    17401 cgccgcccag ggcctggaag agaagaagca gatcctctac ctgctcgggc cggtgggcgg
    17461 gggcaagtcc tcgctcgccg aaaagctcaa gagcctgatc gagcgcatcc ccttctacgc
    17521 gatcaagggc tcgccggtga acgaatcccc gctcggcctc ttcaacgccg ccgaggatgc
    17581 gcacatcctc gaggacgact tcggcatccc gcgccgctac ctcggcggca tcatgagccc
    17641 gtgggcggtc aagcgcctgc acgagtacgg cggcgacatc acgcgcttcc gcgtggtcaa
    17701 gctcggtccc tcggtgctgc gccagatcgc ggtcgccaag accgagccgg gcgacgagaa
    17761 caaccaggac atctcctccc tggtcggcaa gatcgacatc cgcaagctcg agcagtactc
    17821 gcaggacgac cccgacgcct acagctactc cggcggcctg tgcctggcca accgcggcct
    17881 gctcgagttc gtcgagatgt tcaaggcgcc gatcaaggtg ctgcacccgc tgctgaccgc
    17941 aacccaggag ggcaactaca agggcaccga gggcttcggc gcgatcccct tcgacggcat
    18001 cgtgatggca cactccaacg agtccgagtg ggtcgcgttc aagaacaacc gcaacaacga
    18061 ggccttcctc gaccgcatct acaccgtcaa ggtgccctac tgcctgcgcg tctccgacga
    18121 agtgaggatc tacgagaagc tgctcgtcca cagctcgctc gccgaggcgc cgtgcgcacc
    18181 cgacacgctc aagatgatgg cgcagttcgc ggtgctgtcg cgcctcaagg aaccggagaa
    18241 ctcgagcatc ttctccaaga tgcgggtgta tgacggcgag aacctcaagg acaccgaccc
    18301 caaggccaag agctaccagg agtaccgcga ctacgccggg gtggacgaag gcatgaccgg
    18361 gctgtcgacc cgcttcgcct tcaaggcctt gtcgaagatc ttcaacttcg accatcgcga
    18421 agtcgccgcc aacccggtgc acctgatgta cgtgctcgag cagcagatcg aggccgagca
    18481 gttcccgccc gagaccgagg cgcgctatct cggctacatc aaggaatacc tcgccccgcg
    18541 ctacgccgag ttcatcggca aggagatcca gaccgcctat ctcgagagct actccgagta
    18601 cgggcagaac atcttcgacc gctacgtgat ctacgccgac ttctggatcc aggaccagga
    18661 gttccgcgac ccgaacaccg gcgagatcct cgaccgcgca gcgctcaacg aggaactgga
    18721 gaagatcgag aagcctgccg gaatcagcaa cccgaaggac ttccgcaacg aggtggtcaa
    18781 cttcgtgttg cgcgcgcgcg cgaagcacga aggccgcaac ccgagctgga cctcgtacga
    18841 gaagctgcgc gcggtgatcg agaagaagat gttctccaac accgaggatc tgctgccggt
    18901 gatcagcttc aacgccaagg ccagcgccga cgagctgaag aagcaccagg acttcgtcga
    18961 ccgcatgatc gacaagggct acaccgagaa gcaggtgcgc ctgctctgcg agtggtacct
    19021 gcgcgtgcgc aaggcgtcct gattcgcgag ccacggtccg gcccggcccg cgccatcgct
    19081 gcgcggagcc tggccggacg cggacaccgg gaggagacat ggtccgcatc atcgatcggc
    19141 gcttcgacag caagaacaag agcgcggtga accgccagcg cttcatgcgc cgcttcaagc
    19201 agcagatccg caaggcggta tccgaggcga tccacggccg ctccatccgc gacctggaga
    19261 acggcgagca gatctccatt cccgcgcgcg atctctccga gccctctctg caccacggca
    19321 agggcggcat ctgggagcag gtcttccccg gcaacgacca gttcagcacc ggcgaccgca
    19381 tcaagcgccc actcggcggc gctggcgacg gtgccggcaa gggcaaggcg agccaggacg
    19441 gcgagcacga ggacgacttc gtcttccagc tctcgcgcga ggagttcctc gacctcttct
    19501 tcgaggacct cgagctcccc cgcctgatcc gcacccagct cgccaaggtc accgactaca
    19561 agacgcggcg cgccggcttc acctccgatg gcgtacctgc gaacatcaac atcgtgcgct
    19621 cgatgcgcgg agcgctcggg cgcaggctcg cgctcggctc gccctgggct gcacgcatcc
    19681 gcgcgctgca gcaggaactc gacgaagcgc tcgcgcgcgc cggcgaagac agcgaggaag
    19741 tgcgcgacct gcgcgaggaa ctcgccgcgc tgcgcgcgcg catcgaacgc atccccttca
    19801 tcgaccgctt cgacctgcgc tacaacaacc gcgtcaagga gccccgcccc accacccagg
    19861 cggtgatgtt ctgcgtgatg gacgtctccg gctcgatgga cgaggagcgc aagtccatgg
    19921 ccaagcgctt cttcatgctg ctctacctgt tcctcacgcg cagctacgag cacatcgagg
    19981 tcgtcttcat ccgtcaccac accgtggcca aggaagtcga cgaggacgaa ttcttccact
    20041 cgcgcgaatc cggcggcacc gtggtctcca gcgcgctcga gctgatgcgc aacatcctgc
    20101 gcgagcgcta cgccaacggg cagtggaacg tctacggcgc ccaggcctcc gacggcgaca
    20161 actgggacaa cgactcgccg gtgtgcgggc gcctgctcgg caaggagatc ctgccctggt
    20221 gccagtactt cgcctacgtc gagatcaccg ccggcgagcc gcagaacctg tggcgcgagt
    20281 acgccaagct cgaggccgcg cacgacaact tcgcgatgca gcgcatcgag tcgccggccg
    20341 acatctaccc ggtgttccgc gaactgttca agaagacgat cgcatgaaga ccccccgccc
    20401 ccgcacgcgc ggccgcgccg ccaaggctcc gctcccctcc ggctcggaat ggaccgtcga
    20461 ggccatcgac cgctacaacg aggagatcgc ccgggccgcc gcgcactaca agctcgacac
    20521 ctacccggtg caggtcgaga tcatcaccgc cgagcagatg atggacgcct acgcctcggt
    20581 gggcatgccg gtcaactacc accactggtc cttcggcaag cacttcctcg ccaccgagaa
    20641 ggggtacaag cgcgggcaga tgggcctggc ctacgagatc gtgatcaact ccaacccctg
    20701 catcgcctac ctgatggagg agaacacgct caccatgcag ggcctggtga tcgcccacgc
    20761 cgcctacggc cacaacagct tcttcaaggg caactacctg ttccgcacct ggaccaacgc
    20821 cgacgccatc atcgactacc tgctgttcgc gcggaactac atcacccagt gcgaggagcg
    20881 ccacggcgag gaagccgtcg agctgctgct cgactcctgc cacgccttga tgaacctcgg
    20941 cgtggaccgc tacaagcgcc cgcccaggct ctcgctcgcc aaggagaagc tgcgccagca
    21001 agagcgcgag gagtacctgc agagccaggt caacgacctg tggcgcacac tgcccgccca
    21061 cgaactgcgc accgatgccc g
//
LOCUS       DAOQNN010000012        20935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_897_length_20935_cov_11.587835, whole genome shotgun sequence.
ACCESSION   DAOQNN010000012 DAOQNN010000000
VERSION     DAOQNN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 20935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20935
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_897_length_20935_cov_11.587835"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PKV97_01740"
     CDS             complement(<1..1019)
                     /locus_tag="PKV97_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HNB04624.1"
                     /translation="MSTAQQTTALATTSAKTPQAIFKAYLEKSAPAVQQVITKGMALS
                     TDRIIKVMLIAAAKNPKLYDCYQPSIVKSIMDAAALGLELGSPLGHAYPVPYYNKKAN
                     RYDCQLVIGYKGLLKLALGDSIKKIEARVVYENDFFEVELGLREDLRHRPNFDEPGPM
                     IAVYAIATLANGEKQFEVMTKAQVEAIRKRSQNPDSGPWSTDYNEMARKTVTKKLCKY
                     LDLSVQSAEAIAKADEYEEPGEIADPLPDLSQRLGDGATSQGVIDADEVPLSTFDPAP
                     AAAPPPAAAAPAPAAPPVEPPRSAPAVVPQAAAAPAPAASVPPVAPPPAAAVVSAPPP
                     PPVAAP"
     gene            complement(1022..1810)
                     /locus_tag="PKV97_01745"
     CDS             complement(1022..1810)
                     /locus_tag="PKV97_01745"
                     /inference="COORDINATES: protein motif:HMM:NF021128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HNB04625.1"
                     /translation="MDARTFEERRRYLGSSEVPAVCGVDPFKCAADIWAHKMGYVDQD
                     DSDAAAIGNLLEAPLIEEYARRVGAKTIDKPGTIRRWYRQATPDAIVDGINTQVKVVG
                     FHMRHHWDEGCPDYVQAQVQSEMDLTGLGLTDVVVLLGTEFRVYRIERDDAAIRKISA
                     ICDSFWRRFIVGREQPPIQKSRNANSILARIHPKHKGEVSAVQGVIDAAKEYESLGAQ
                     IRDLTEKQKDLGILLKLAVGDNKSLTWPGGRVSWSQKLTVTIKE"
     gene            complement(1823..2362)
                     /locus_tag="PKV97_01750"
     CDS             complement(1823..2362)
                     /locus_tag="PKV97_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04626.1"
                     /translation="MTPDQLQSLLDALAGDFRALSIWPEWIYAFCFLNKRIENRGPTV
                     ARDARRHIGEWIGLHGSKRIGGKPPNFYGNWNQNHANAVKEMTATARAAGEKPGQVPL
                     RDIIDCRGVRAIGYLSEVLPPPRQRSGYHMPDCYGLRFSEVIVLPSAVEAKGALGFWR
                     VPNEVKAEIRNHLLATKQV"
     gene            complement(2584..3222)
                     /locus_tag="PKV97_01755"
     CDS             complement(2584..3222)
                     /locus_tag="PKV97_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04627.1"
                     /translation="MQKRISERRHAQRRGLQRAGIWYDGNDLAEMERMIRAGQFIEAH
                     RDTNTRSIITLIFRERTHRVVYSKNTKQIITFLDGLKPRQAESTALQAFKILLADSAL
                     LASNSIDGSALKWSYIASSLELITMLPFPVFFEAGHVLAGMRAQKNETCDALAAMLEE
                     RQIKPLGGARDLWAHIVRVARRAANEAEDKALSRRLMNVSEVYRSGMENLDE"
     gene            complement(3225..3590)
                     /locus_tag="PKV97_01760"
     CDS             complement(3225..3590)
                     /locus_tag="PKV97_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04628.1"
                     /translation="MTEIQLGQEWREKKYAQRLMRVEELKLDQKGSLTHVKLLLLEGQ
                     GRPSTFIQPYILRSSFVLEKGAPDAPRRKVVLELADNTTEKDCGACKYLNADAENNCH
                     VFGGIEGTKRSSLCIAAEK"
     gene            complement(3594..4526)
                     /locus_tag="PKV97_01765"
     CDS             complement(3594..4526)
                     /locus_tag="PKV97_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04629.1"
                     /translation="MSSSTADQECKNRIQRAKELSEGATADDFDFIDDEITTRRGGHL
                     IAKIKQHGTSGDGRLFAEARTLLPAIAADYEKLQEDLGKVATEASDLADNCKEHRLNH
                     EAEKERAEGLRRLLERTSDDLKAALAREQERAQKLHRRLITAEAALGTKREIAGVMEE
                     NRELRENNRRLKAYAERTQKQAADDRVALDKARAEIEALHRKEQAQCIYCDALLPKAE
                     LIAHLRNCGMQSEAMAAREREISKLRQDLLIVKNSQDAEKTELHADLNATTKAVSAVR
                     FVRGCKCGFAARGNCEHYHGLFGFPLPPLPVGKE"
     gene            complement(4529..4696)
                     /locus_tag="PKV97_01770"
     CDS             complement(4529..4696)
                     /locus_tag="PKV97_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04630.1"
                     /translation="MRIPKGKKITPEDHAEIAKFRAFLAAGKPQQQAYAERYGEVVFE
                     AGADDKAPETE"
     gene            complement(4912..5295)
                     /locus_tag="PKV97_01775"
     CDS             complement(4912..5295)
                     /locus_tag="PKV97_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04631.1"
                     /translation="MRHHLGLNAAMLATLTRMAPRPWNPHVERLEAAGFINPWGVYED
                     LIGIAENDRLGRSVTRFVDICIEVFARAAEMRERRFVLTAPKFEAPPLPEPHEIRMPR
                     WRNFYESPNRGRGAQRRREKPWSRR"
     gene            complement(5259..5930)
                     /locus_tag="PKV97_01780"
     CDS             complement(5259..5930)
                     /locus_tag="PKV97_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB04632.1"
                     /translation="MTTTALQQRQHELWQLRRDSSAMKELIETMQGGIIDLARRFSVM
                     SDMDRHDLESECYMAFLHWVERWNPDGGKPLFGFLYERMAWRLMRYTKEQRHTIRLPE
                     STFHKKHVVVKCRSMDARIKSDDGERQNLSDVLFDAEVEPADVTCQRAFNVAKVREIL
                     ATMSEREQWILQERADDRTLEEIGKDLGLCRERVRQIEEQALDKLRRRYRKACAITSA
                     SMQRC"
     gene            complement(6310..7233)
                     /locus_tag="PKV97_01785"
     CDS             complement(6310..7233)
                     /locus_tag="PKV97_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04633.1"
                     /translation="MVAAARCAASPETEEPPQHRDPREEKEPSIMGDKLKDLGFNAGR
                     KDVDTIDPKDLSLVWHKKTPEMGPNDFVVCPDLEWAFDASIENPINPEHVELALTKQG
                     GKYVGILEPIGYRKVEYKGKLRLFVVFGRGRTRMAREANARIKKLGGSEADFIFVPAI
                     VKTITDTEAAILRDVENLHRKILKPIDLARLAQQHIDQGTPEPIVLKLFGVKSMQQVK
                     NYAALLKQPEPVQRLIADGKIPMSAKIEPKQAAKVVEAAEKSEAPLSMRDIAEIQGTA
                     KSKMMTRGAIEKWHEKASGIEKATLAKVLGL"
     gene            complement(7424..7612)
                     /locus_tag="PKV97_01790"
     CDS             complement(7424..7612)
                     /locus_tag="PKV97_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04634.1"
                     /translation="MQTARKMVLLRGDQREWLAKFPWAGCKMVRACIDAGRVLFELGF
                     WKGKDFDLDRLREKLVKK"
     gene            7656..8060
                     /locus_tag="PKV97_01795"
     CDS             7656..8060
                     /locus_tag="PKV97_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNB04635.1"
                     /translation="MSSVNKVILLGNLGKAPELKNLPSGQTVCNFSIATSETYKDKNE
                     QKQERTEWHNIVVWGKTGENCAKYLDKGSKVYIEGKIQTRTWDDKEGKKQYRTEINAM
                     SIVFLDGRSGGGGPRPSNAPDNLPPADDEIPF"
     gene            8080..8532
                     /locus_tag="PKV97_01800"
     CDS             8080..8532
                     /locus_tag="PKV97_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04636.1"
                     /translation="MENENQTPPQTPAPGLTSGLDPEPPRYEFVELFGHNCLGPCEVR
                     EVSRYGATFCEARPLKQDGTVGASQLIAGSAIYRVQPLSEQMAKLIAARRLGYVPLAL
                     PAVSETGGDGTRPDPPDGDKDGNRRDFDDDDGHDEDEADRGNDDDIPM"
     gene            8546..8911
                     /locus_tag="PKV97_01805"
     CDS             8546..8911
                     /locus_tag="PKV97_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNB04637.1"
                     /translation="MPAPLPDRSRAESISTLQALLPLHLGLYLAAKQAHWAVRGSLFL
                     QLHKLFDKVAKTAANGADTIAERIRQLGGETTPVTTDPLIPGTDGSNSRANSARHWPT
                     SAPRTSPRSRCLSAARTMR"
     gene            complement(8987..9214)
                     /locus_tag="PKV97_01810"
     CDS             complement(8987..9214)
                     /locus_tag="PKV97_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04638.1"
                     /translation="MTRELLALMTVAIPPLLFGLRTGAYASGALVILCLIRAVVLVVA
                     RRIEKERRLRWIDEKLRQAERETPPATKPQQ"
     gene            complement(9211..9501)
                     /locus_tag="PKV97_01815"
     CDS             complement(9211..9501)
                     /locus_tag="PKV97_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04639.1"
                     /translation="MTIVTGLVWFLGTGGVLLADPEPPPKPQAHIPFGPLAPSCDLCD
                     QSDRAACGWRPSLCALQSSATVAIYCCVSSAVAAPEAPPVDYTPAAPEPISQ"
     gene            complement(9536..10489)
                     /gene="rdgC"
                     /locus_tag="PKV97_01820"
     CDS             complement(9536..10489)
                     /gene="rdgC"
                     /locus_tag="PKV97_01820"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HNB04640.1"
                     /translation="MNLTRFAAKGTLPKNVERRIAAHAAVPIQPETNTERAVGWCQHG
                     DELNLHPAWNYNNIVLMDLRIETLKPAGKDLKRLVRQRVAELEAELKEPLPKFRKIEI
                     RDVLKMDLRKKIPSKISTVPVLWDLARNRVYLYTQSQSVIDSFLLVFGQTFEVPTDIE
                     GTLAWNDKADLHTAPKFLTWLFYKSSQNNGEWMLPGLDRFVVQMGEKFLLGHGEAVAA
                     FNGDADQARRAIAEGYSVRELPLCIFIGDKLWMGTLNTALQWKGVKLPTILGETPEET
                     AMETSELLAELDGMLRGMFAAFLENAIDENAIINWSKADRT"
     gene            10592..13558
                     /locus_tag="PKV97_01825"
     CDS             10592..13558
                     /locus_tag="PKV97_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HNB04641.1"
                     /translation="MSAIDLRRQLTDVQQVLDGCGYRGEIVKEGRGVKIRCPWHADDG
                     RPNLQISMRSDGTLGVKCFSCQKGGDILHLIGELTGKGDPKTNFKAVAQEAERIAGTR
                     MTSAPAPRTSSTADNFPDKLEVAKFWNLTVDLQTNADLCNRLTARHIDPQLIKELARA
                     LPDLSKNPRWGWVRNPDPSGDPLTWAKAGYRLIVPMYDEAGELVSVHARRFLGADDKQ
                     KGTSPSRCRIAGSMMLSPAALQSAVWDDVIFVEGVPNYLILASLYPTRPVVGVISGSI
                     KGCTFPRIPRGASVTVWMDADAQRNRAGQEIEGAGQIYTNHALAALRGRRIRVVKLAI
                     PEGKKKAPDANDIYIAGGGQMISDVLSQAETNAAPLDPKAEAMWNRGAGVYGMQRPPL
                     NMDRLEDREIVRLIDSKLETRRWNEFGELIGIGEGPGGWFANQQGLHFLRTGEAPPAG
                     IVVTQGFENFLALSQLWDDDAVAMEPAILAGLPPKTTSRLVLWPSQPCEQDHEVVRAS
                     EPRVAAERGNKAMFACLSAAQPVPTVLPIPPSEKEAKQFMRTDLGNAERLAHLYGDDI
                     HYVKDWDSFLVWDGKRWARDRTFEVDRRAAYVVRQLYDGDEKDRAWAHDSESAGKLSA
                     MVSIVRKLPGVPILHEQLDADLYLLSVANGTLDLRTGELLTHSRDHLHTKRADVIYDR
                     KAKCPKWHEFLRTIFDGNTNLISYIQRAAGYSLTGDVGEHVLFMLYGTGRNGKSTFLM
                     TLQSLLADYSKAISSEVLMSTGRKLDAGQQSAIASLRGARFVVSSETEEGAKFSESVA
                     KQLTKGDIIQAKFMGRDVFDFTPTHKLWLATNHRPKTRGKDDGLWTMLIPVPFTVTIP
                     IEKRDRTLPAQLLAERSGILNWALDGCLDWQVNRLGRPAEIDAAVNSYRKEMDQIGPF
                     IEQCCIVGADHQVKISALRKAYESWCSETSKEPLSVRTFGSILEEKFPADSTGDYRFR
                     RGLMLRGEWVATNERN"
     gene            13545..14195
                     /locus_tag="PKV97_01830"
     CDS             13545..14195
                     /locus_tag="PKV97_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04642.1"
                     /translation="MSETDLARLALRQLEEMGFDCYQEVAHGAHRADIVGVRRGIVCI
                     VECKTALSFDVVAQADDWLPVAHWRFIAVPMARYAGRGRELAYAICEQRGIGVLEISE
                     QHDRVWIRRYPALNRAAKPQRLLDALRPEHKTYAAAGSSGSYWSPWKSSVQSLVLEVN
                     RKPGRPLRDVIAHIKHHWVSNKSAVSCVSRQIADGAIEGLRLEKKGRAVLVFPTAK"
     gene            14277..14621
                     /locus_tag="PKV97_01835"
     CDS             14277..14621
                     /locus_tag="PKV97_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04643.1"
                     /translation="MTAAIKATRAAGYKLLGKLAEVMNITAHLGHLTEYPLVVANLQK
                     LRGIVISHISDIGDPERWHTFEDVPNLDRLHVVDSIEELNRHPAKHEALLAQLAAEAA
                     AANEILFGKGAA"
     gene            14622..14837
                     /locus_tag="PKV97_01840"
     CDS             14622..14837
                     /locus_tag="PKV97_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04644.1"
                     /translation="MKTVFTIVYDAPELGPGPNADDTHTKRFTNKKQAETFAAGKTYY
                     GQPVTVQEDNNVPPKLFQRWKREGKIG"
     gene            14834..15025
                     /locus_tag="PKV97_01845"
     CDS             14834..15025
                     /locus_tag="PKV97_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04645.1"
                     /translation="MKVLALPYRLEAVPDPVLGKIGIAVGTFASAKDAANFFQRYPNA
                     ATPCWQRMEVIDGELVQVR"
     gene            15030..15776
                     /locus_tag="PKV97_01850"
     CDS             15030..15776
                     /locus_tag="PKV97_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04646.1"
                     /translation="MNLGHINFHRLTDSDLASIWAAIETQPARPEALEMIDAPPRCTM
                     KGLLKRLAQAEPQHEAKAPLVVSCGIGVDSVAMLVGLWRRGIRPDLILFADPGSEWPE
                     TYLYVLVLAAWLAEVGFPPLTIVRRDQGRTSYTTLEGNCLVNRTLPSMAFGYGGCSQK
                     WKREPMDKLVGRWYPRCARYELVSRPSARSATTLAPKTAGAATSKTAPATTTFTSCAS
                     GAGTATAAKLRSSPPACRCRARAHASSAQR"
     gene            15773..15952
                     /locus_tag="PKV97_01855"
     CDS             15773..15952
                     /locus_tag="PKV97_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04647.1"
                     /translation="MKAWELRDLIRLHPALADRVIAIESGAAKALIKVEGLWRKATKE
                     KPGSMTAFINAERAN"
     gene            15949..16188
                     /locus_tag="PKV97_01860"
     CDS             15949..16188
                     /locus_tag="PKV97_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04648.1"
                     /translation="MTKEPNILSVRVGGAGGTVYHNVRLHGSGGLCPDREAAIKLLEK
                     RQKRGEKIMLHHNYSAQGSDKTHHDAVDVEILPKE"
     gene            16275..17546
                     /locus_tag="PKV97_01865"
     CDS             16275..17546
                     /locus_tag="PKV97_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04649.1"
                     /translation="MTQIAVVYPTSEDAIATLRERYTGLSADTPKGYEEVRVAIGELR
                     KLRVAVEERRKELKKDSLEYGRRVDSVAKALTEQLEAIENPLKLLKEAADEEKARAKA
                     EKEAAERREFEEKLRAEQEAREQRLAEIRAQEEARIRAEAEQLAQERAAFEAAERERA
                     AAMEAQLLEAERQEAERQRLAQIEREQIEAERQRIAEEAAEMQRQQEAIVAERQRLAD
                     EQAAAEARAQEELRRQQEELAAERAEIERQRAELAKQAEEEAAQRELERQRRAAQEEA
                     DRQAAAQLAEQQERERVAQEQAQREAAEAAEAAEAAAPQAVPVPIDNDDETFSIADLG
                     LGSSSTSAKICEHEKFKTSVDVTRLTSGGDSEPTSYTAEIRIHCAACGVPMKFLGLQG
                     GSHPERPTVSFDGLELRCPVIPSSDKAEGQA"
     gene            17549..17917
                     /locus_tag="PKV97_01870"
     CDS             17549..17917
                     /locus_tag="PKV97_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04650.1"
                     /translation="MASREGRLVAAAPAELRRQVVATGDTELFGGFAIDPFRVPTTDE
                     EIAHKRNAMAFIEGNYPLSIGDCEVVGISGGCGEGCPILKRGACETQAEMEHALAQLD
                     LNRLSRAWLRDCLQAAMEMR"
     gene            17914..18201
                     /locus_tag="PKV97_01875"
     CDS             17914..18201
                     /locus_tag="PKV97_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB04651.1"
                     /translation="MKTTTAATQASAQARLERELCEPPTDPAPGAAGAGVRIRTARLL
                     RGISQAQLGEAARVSEPTIIGIEQGRSWRGDVLERIAKALDMRAGELAGWL"
     gene            18192..18983
                     /locus_tag="PKV97_01880"
     CDS             18192..18983
                     /locus_tag="PKV97_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04652.1"
                     /translation="MAVILRVGIPKPNGSMTRELHRRRQPVLISAGALWDPRTEAFRC
                     SGLLLLRMDCALDSAGFVRGLSGYPWSVEQYVELAAGSSWAWWAQMDLPCEAAIAPDR
                     ETVVRRVIESAALFRRCAAAAETARQASAFLRETFADPMPVLQGRRLEDYQRSIELLS
                     PLPALVGVGSMCTRPIHGPEGLVPIVEGLHQLLPASVQLHLFGVKGPALAALERFGDR
                     ILSVDSMAYDIHARKTAHREQVSNTVARRTSVMLDWISKQGPCQR"
     gene            complement(18984..19220)
                     /locus_tag="PKV97_01885"
     CDS             complement(18984..19220)
                     /locus_tag="PKV97_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04653.1"
                     /translation="MTRLFFALFLSGCATAPTTTPTVEYFGRCTERVKSGTPDIRDMD
                     YRRSTLAAAAADIAAFRQNFPGYACVIMEAPRTE"
     gene            complement(19259..19435)
                     /locus_tag="PKV97_01890"
     CDS             complement(19259..19435)
                     /locus_tag="PKV97_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04654.1"
                     /translation="MSDRKIPDRCPEIDAAEAQLGRHLTSAEKADLLEGRAAVVQGQL
                     VESSTGTHANDARK"
     gene            complement(19425..19937)
                     /locus_tag="PKV97_01895"
     CDS             complement(19425..19937)
                     /locus_tag="PKV97_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04655.1"
                     /translation="MDAGQVVKQAWGRYIQPYVASVQSARQGNPVDAAAERWLRKMAE
                     RPKDAETWAEWGRQTARRHCLHDSLHVQMAIDFDHRPRLLLYPQTELEEVLRIAGEAC
                     CEELTRLWPAISAARDHLGDKEAPAFVALLDLVSLEHQTEFLQRISGYAAELSVDAEF
                     RLRAEVRHVG"
     gene            complement(19919..20503)
                     /locus_tag="PKV97_01900"
     CDS             complement(19919..20503)
                     /locus_tag="PKV97_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04656.1"
                     /translation="MSKDQKPTPATEKTPAASTSATTTATSLETSSTTTAAETKPAGE
                     KPAETKSTSLPATTPTGESPKAPQFEKLPDDRWPLNRQEPVDPKGDAELSAEQVTLVD
                     KGVAFLLMSKDHWKMGVPEPLSAWTTLRELLPSGTRAQVESDIDLSALRRILSDQIIC
                     RIYHGITSKERLLVVRCVGTKYSRMEIIPWTLGK"
BASE COUNT         3875 a         6568 c         6529 g         3963 t
ORIGIN      
        1 ggcgcagcca cgggcggcgg cggcggcgcg ctgacgacag cagcagccgg cggcggagca
       61 acggggggaa ccgacgccgc aggcgccggt gcagcggcag cctggggcac cacggccggc
      121 gcggaacgcg ggggctcgac cggcggtgcg gcaggagccg gggcggcggc agcgggcggg
      181 ggagcagcag ccggcgcagg atcgaaggtc gacaacggga cctcatcggc atcgatcacg
      241 ccctggctgg tagctccatc acccaggcgc tgcgatagat ctggcagcgg atcggcgatc
      301 tcgcccggct cttcgtactc atcggccttt gcgatggctt cggcgctctg caccgacaga
      361 tcgaggtact tgcagagctt cttcgtcacc gtcttgcggg ccatctcgtt gtagtcggtc
      421 gaccacgggc cgctgtcggg gttctggctg cgcttgcgga tcgcttcgac ttgagccttg
      481 gtcatcacct caaactgctt ctcgccgtta gccagcgtag cgatggcata gaccgcgatc
      541 atcgggccag gctcatcgaa gttcgggcgg tggcgcagat cttcgcgaag gccaagctcg
      601 acctcgaaaa agtcgttttc gtacacgacg cgcgcttcga tcttcttgat cgaatcgccg
      661 agagcaagct tgagcaggcc cttgtagccg atgacaagct ggcagtcgta gcggttcgct
      721 ttcttgttgt agtacggcac cgggtaggcg tggccaagcg gcgaacccag ttcgagcccc
      781 aacgctgccg catccatgat gctcttcacg atgctgggct gatagcagtc gtagagcttt
      841 gggttcttgg ccgccgcgat cagcatcact ttgatgatgc gatcggtcga cagtgccatc
      901 cccttggtga tcacttgctg cacggccggc gcgctcttct cgagatacgc cttgaaaatc
      961 gcttgaggcg tctttgcgga agtggttgca agggcggtgg tttgctgtgc ggtactcatg
     1021 tttattcctt gatggtgacg gtgagtttct ggctccaact gacgcggcca cctggccatg
     1081 tcaggctttt gttatcgcca acggcgagct tcaaaagaat gccgagatcc ttctgtttct
     1141 cggtgaggtc acgtatctga gcgcctagcg attcgtattc cttcgcggcg tcgattactc
     1201 cctgcactgc ggagacctcg cctttatgct tcggatgaat ccgagcaagg atgctgttcg
     1261 cgttacgact tttttgaatc ggtggctgct cgcggccgac aatgaaccgc cgccagaagc
     1321 tgtcgcagat cgcggagatc ttccgaatcg ctgcatcgtc cctctcgatg cggtacacgc
     1381 ggaactcggt gccgagcagc accaccacat cggtgagccc aaggccagtg agatccattt
     1441 cggattgcac ctgcgcctgc acgtagtcgg ggcagccctc atcccagtgg tgacgcatat
     1501 gaaagcccac taccttcacc tgcgtgttga tgccgtccac gatggcgtct ggcgtggcct
     1561 gcctgtacca gcggcggatg gtgccgggct tgtcgatggt cttggcgccg acgcggcgcg
     1621 cgtactcttc gatcagcggg gcttcgagca ggttcccgat ggcggcggca tcgctgtcgt
     1681 cttgatcgac gtagcccatc ttgtgcgccc agatatcggc ggcgcacttg aacgggtcca
     1741 ccccgcagac tgctggcacc tcggaagacc cgaggtagcg tcgcctctct tcgaacgtcc
     1801 gcgcatccac aaaaccactt tactagacct gtttagttgc tagcaagtga tttctgattt
     1861 cggccttcac ttcgttgggt acgcgccaga acccgagcgc gcccttcgct tcaaccgcag
     1921 agggcagcac gatgacttcc gagaagcgca gcccgtagca gtcgggcatg tggtagcccg
     1981 agcgctggcg cggtggtggc agcacttcgg agaggtagcc gatcgcgcga acaccgcggc
     2041 agtctatgat gtcgcgcagt ggaacctgcc caggcttttc accggccgct ctggcggtgg
     2101 cggtcatctc tttcacggca ttggcgtggt tctggttcca gttgccatag aagttcggag
     2161 gcttgccacc aatgcgcttg cttccatgca gcccgatcca ttcaccgatg tgccgtcgag
     2221 catcgcgtgc gaccgttgga cctcggttct cgatgcgctt gttcaaaaaa cagaacgcat
     2281 agatccactc gggccaaatc gacagcgctc ggaagtcacc ggcaagagca tcgaggaggg
     2341 attgaagctg atcaggtgtc atctcacttc gcctttgtgc agggccttgg ccgccgctcg
     2401 tcgtactttg ggatcagcca gtgctcatcg accgcaggac ccagcactac ggcagcgcat
     2461 tctggattgg gacacggccc atggccgttg ccatagatca cggtcccctt tgccgagcag
     2521 cgaactaatt cagatggtgg accgtcctct cggcgccgtt gcttctgggc cttcggattc
     2581 tttttactca tccaggtttt ccatgccaga tcgatagact tcgctgacat tcatcaggcg
     2641 ccggctcagc gctttgtctt cggcctcatt ggctgctctt cgtgcgaccc taacgatatg
     2701 agcccataga tcccgagctc cgccgagcgg cttgatctgc cgctcctcga gcatcgcggc
     2761 cagcgcgtca caggtttcgt ttttctgcgc tcgcatccca gcgaggacat gccctgcctc
     2821 gaagaagacg ggaaacggta gcatcgtgat gagttcgaga ctggatgcga tgtagctcca
     2881 cttgagcgcg ctgccatcta tgctgttgct ggcgagtagc gcggaatcgg cgagcaggat
     2941 cttgaacgct tgcagcgccg tgctctcggc ctgtctcggt tttaggccat cgaggaaggt
     3001 gatgatctgc ttcgtatttt tgctgtagac aacccggtgc gtgcgctcgc gaaatatcag
     3061 cgtgatgatg ctgcgcgtgt tcgtatccct atgcgcttcg atgaactggc cggcgcggat
     3121 catccgctcc atctcggcaa ggtcgttgcc gtcgtaccag atgccagcgc gctgaagtcc
     3181 gcggcgctgc gcatggcgcc gttcgctgat tcgtttttgc atcgctactt ctcagcggcg
     3241 atacagagag atgaccgctt cgtgccttcg ataccaccga agacgtggca gttattctcg
     3301 gcgtcggcgt tgaggtattt acaggcaccg cagtctttct ctgtggtgtt gtctgcaagc
     3361 tcaagcacga cttttcgccg tggtgcgtcg ggtgcgccct tttccaagac gaaggacgag
     3421 cgcaagatgt agggctgaat gaaagtgctg ggcctgcctt ggccttcgag cagcagcaac
     3481 ttgacatggg tcaacgagcc cttctgatcg agcttcagtt cttccactcg catcagccgc
     3541 tgcgcatact tcttctcgcg ccattcttgc ccaagttgga tctctgtcat cgtctactct
     3601 ttccccaccg gcagcggtgg cagcgggaac ccgaagaggc cgtgatagtg ctcgcagttg
     3661 ccgcgggccg cgaagccgca cttgcagccc cggacgaacc gcaccgcgct cacagccttg
     3721 gtggtggcat tgagatcggc gtgcagctcg gtcttttcgg catcctgcga gtttttgacg
     3781 atgagaagat cttgccgcag cttcgagatc tcacgttctc gcgcggccat ggcctcggac
     3841 tgcatgccgc agtttcgaag atgcgcgatc aactcagcct tcggcaaaag cgcgtcgcag
     3901 tagatgcact gggcttgttc tttccggtgc agcgcttcga tctcggcgcg ggccttgtcc
     3961 agagcaactc tgtcatcggc tgcctgcttc tgcgttcgct ctgcatatgc cttcagccgg
     4021 cggttgttct cgcgcaactc gcggttttct tccatgacgc cagcgatctc gcgcttggtg
     4081 ccgagcgcgg cctcagcagt gatgaggcgg cgatgcagtt tctgcgcgcg ttcctgttcg
     4141 cgggcaaggg cggctttcaa atcatccgag gtgcgctcaa gcaggcggcg caacccctcg
     4201 gcgcgttctt tttctgcctc gtgattgagg cggtgctctt tgcagttgtc ggcgaggtcg
     4261 ctggcttcgg tcgcgacctt ccccagatct tcctgcaact tctcatagtc cgccgcgatg
     4321 gccggcagca gggtgcgggc ctcagcgaac aaccggccgt cgccgcttgt cccgtgctgc
     4381 ttgatcttgg cgatgaggtg gccaccacgg cgggtagtga tctcgtcgtc tatgaaatcg
     4441 aagtcgtctg cggtagcgcc ttcgctcagc tccttcgcgc gctggatgcg gtttttgcac
     4501 tcttggtctg ccgtgctgct cgacatgatc actccgtctc tggcgctttg tcgtctgcgc
     4561 ctgcttcaaa cacgacctcg ccataccgct cagcataggc ttgctgctgc ggcttgccgg
     4621 ccgccaggaa ggcgcggaac ttggcgatct ccgcatggtc ttctggcgtg atctttttac
     4681 ccttcgggat cctcatcgtt cctcggtgcc acttccggcg cgctcattgg caatctctcc
     4741 ttcgtggccc cttgtcctgc caccgtttcc aaagccagcg caccgtcttg atttgggcct
     4801 tcgtgcagcc ggcttgccgt gctcgcatcg ccgacccata gagcgatccg tctacatagg
     4861 ccgataggtc gcgcaccata cgacgtttct gcttgtccca cgccttcggc atcatcggcg
     4921 agaccacggc ttctcgcgtc ggcgctgtgc tccgcggcct ctgtttggag attcgtagaa
     4981 gttcctccag cgcggcatgc ggatctcatg gggttccggc agcggcggcg cctcgaactt
     5041 cggcgccgtc agcacgaacc gccgctctcg catctcagcg gcgcgcgcaa agacctcgat
     5101 acagatgtcg acaaatcgcg tcaccgagcg gccaaggcga tcgttttccg cgattccaat
     5161 cagatcctcg tatacgcccc atggattaat aaagcccgcc gcctcgagcc gctcgacgtg
     5221 tgggttccat gggcgcggag ccatgcgagt gagggtcgct agcatcgctg cattgaggcc
     5281 gaggtgatgg cgcatgcttt tcgatacctc cgtcgtaact tgtcgagggc ctgttcttcg
     5341 atctgccgga ctcgttcgcg gcacaggccc agatctttac cgatctcttc gagcgtcctg
     5401 tcatcggccc gctcttgcaa aatccactgc tcgcgctccg acatcgttgc gagaatctct
     5461 cgcaccttgg cgacattgaa cgcgcgctgg cacgtcacat ccgcgggctc gacctctgca
     5521 tcaaacagga catcgctcag gttctgccgc tcgccgtcgt cgcttttgat ccgcgcgtcc
     5581 atggatctgc acttcaccac gacgtgcttt ttgtgaaacg tcgactcggg tagccggata
     5641 gtatggcgtt gttctttggt gtagcgcatc aggcgccacg ccatgcgctc gtatagaaaa
     5701 ccaaacaatg gctttccgcc atcggggttc cacctctcga cccagtgaag gaaggccatg
     5761 tagcattccg actcgaggtc gtgacggtcc atgtccgaca tcaccgagaa acgacgcgca
     5821 aggtcgatga tgccgccctg catcgtttcg atgagctcct tcatcgcact tgaatcgcgg
     5881 cggagctgcc agagctcgtg ctggcgctgc tgaagtgcgg tggtggtcat acgacccacg
     5941 agaacggaaa aatctcaggg ttcggcttca tctctttttt ggtgagccgc tgctgtccct
     6001 tcggatgaac tccgctgcca tcgcaggggc gccacttcgt tttgtttttc ttcggccgag
     6061 tgccgcaggg ctcgcatgcc acgcacatat gcccaggccc atgtcctgtg gcgcagcggc
     6121 ccgtgttctg accccaacac agcgcgcaga gtgagagacg ctcaccgatg cgatagatgt
     6181 ctcgcagtag cttgagcttg gcagggtccg cctttttgcc gtccagcagc ttcacaacgg
     6241 ctgcgcatgc gcgagctgcc gcccgcttcc gcgcgccctg cgtcatgccg atgagggacc
     6301 gcggatccac tacagcccca gcaccttggc aagcgtcgcc ttctcgatgc cgctcgcctt
     6361 ctcgtgccac ttctcgatcg cgccgcgggt catcatcttc gacttcgcgg tgccttggat
     6421 ctcggcgatg tcgcgcatcg agagcggggc ctcggacttc tcggcggctt ccaccacctt
     6481 cgccgcctgc tttggctcga tcttcgccga catcgggatc ttgccgtccg cgatgaggcg
     6541 ctgcactggc tcaggctgct tgagcaaggc cgcgtagttt ttcacctgct gcatcgactt
     6601 cacgccgaac agcttcagca cgatcggctc gggcgtgccc tggtcgatgt gctgctgcgc
     6661 gagccgcgcg agatcgatcg gcttgaggat cttgcgatgc aagttctcga catcgcgcag
     6721 aatcgctgcc tcggtgtcgg tgatggtctt gacgatcgcg ggaacgaaaa tgaaatccgc
     6781 ctcgctgcca ccgagctttt tgatgcgggc gttggcttcg cgggccatcc gggtgcgacc
     6841 acggccgaag acgacgaaga gccgcagctt gcccttgtac tcgaccttgc gatagccgat
     6901 cggctcgagg atgccgacgt acttaccgcc ttgcttggtc agcgcgagct cgacgtgttc
     6961 ggggttgatc gggttttcga tcgacgcatc gaacgcccac tcgagatcgg ggcagaccac
     7021 gaagtcgttt ggccccatct cgggcgtctt cttgtgccag accagcgaca gatcttttgg
     7081 atcgatcgtg tctacgtcct tgcggccggc gttgaagccg aggtctttga gcttgtctcc
     7141 cattattgaa ggctcctttt cttcccgagg atctcgatgt tggggtggtt cttcggtttc
     7201 aggtgacgct gcacaacgtg cagccgcgac caccgagagg ccaactggtt cgagcggaag
     7261 aagtcatcgt cggtcttccg cgatcgatct gcgtcgagtc gctggttgtg ccgccacgct
     7321 ttgcgcaacc gagtgatggc gcgcgcgagc tggccgctgt ggtgccgcag atcgcgcagg
     7381 gtcttgccct tctgcttggc cagcttcctc gctatgcgca gtctcatttt ttcaccagct
     7441 tctcgcgcaa ccgatcaaga tcgaagtcct tgcccttcca gaaccccagc tcgaacagca
     7501 cgcggccagc atcaatacag gcgcgaacca ttttgcaccc cgcccacgga aacttcgcga
     7561 gccactctcg ctggtctcct cgaagcaaaa ccatcttgcg tgccgtctgc ataaaagtta
     7621 tataccgcta gcaactaaaa ggagcaacct gaaaaatgag cagcgtcaac aaagtcattt
     7681 tgctgggcaa tcttggcaag gcgcccgaac tcaaaaacct gccgagcggg cagacggtct
     7741 gtaacttttc gatagcgaca agcgagacct ataaggacaa gaacgaacag aagcaggagc
     7801 gcacggagtg gcacaacatc gtggtctggg gcaagaccgg cgagaactgc gccaagtacc
     7861 ttgacaaagg cagcaaggtc tacatcgagg ggaagattca gacccgaacg tgggacgaca
     7921 aagaaggcaa gaagcagtac cgcaccgaga tcaacgcgat gagcatcgtc tttctcgatg
     7981 ggcgcagcgg cggcggtggc ccgcggccct cgaacgcacc cgataactta ccgccggccg
     8041 acgacgagat cccgttctag gaaaaggaaa ccgagcccta tggaaaacga aaaccagact
     8101 cccccgcaga ccccagcccc tggcctgact tcgggcctcg atcccgagcc gccccgctac
     8161 gagttcgtag agctctttgg gcacaactgc ctcggtccct gcgaagtgcg cgaagtcagc
     8221 cgctacggcg ccaccttctg cgaagcgcgc ccgctcaagc aggatggcac ggtcggggcc
     8281 tcgcagctca tcgccggcag cgcgatctac cgcgtgcagc cgctgtccga gcagatggcc
     8341 aagctgatcg cggcgcggcg cctcggctat gtgcccttgg cgctgccggc cgtgtccgaa
     8401 accggcggcg acggaacccg tcccgatcct cccgacggag acaaggacgg caaccgccgg
     8461 gatttcgacg acgatgacgg gcacgacgaa gacgaagccg accgcggcaa cgacgacgat
     8521 atccccatgt agtaagctcg gccgcatgcc ggccccactc cccgatcgtt ctcgcgccga
     8581 atccatttcc acgctgcaag cgctcctgcc gctccatctc ggtctctacc tcgccgcaaa
     8641 gcaagcccac tgggcggtgc gcggatcact cttcctgcaa ctgcacaagc tcttcgacaa
     8701 ggtggccaag accgcggcaa acggcgctga caccatcgcc gaacggatcc ggcagcttgg
     8761 cggcgagacc accccggtca cgaccgatcc gttgataccg ggcaccgatg gctcgaactc
     8821 gcgcgccaac tcagccaggc actggccaac atctgcgccg cgaaccagtc cgcgatcgcg
     8881 atgcttatca gcagcgcgca cgatgaggtg agtgcaaact tactcaccga tgttgagggg
     8941 aagctcgaga agatgctgtg gatgttggaa agcgtggtgg cgcctgctac tgctggggct
     9001 tcgtagcggg cggtgtctcg cgctcggcct gcctgagctt ctcatcgatc cagcgcagcc
     9061 ggcgttcctt ctcgatgcgc cgcgctacca ccaagaccac agcacggatc aagcagagaa
     9121 tcaccagcgc gcctgaagcg taggcacccg tgcgcaggcc aaacagcagc ggcgggatcg
     9181 ctaccgtcat gagcgcgagc agctcccgcg tcattgcgaa atcggctcag gcgcagcggg
     9241 cgtgtaatcg accggcggag cttccggcgc cgcgacggcg gacgacacgc agcagtagat
     9301 cgccacggtc gcggagctct gaagcgcgca caggctcggc cgccagccgc aggccgctcg
     9361 atcgctctgg tcgcagagat cacaggacgg cgcgagcggc ccgaagggga tatgcgcctg
     9421 cggcttcggc ggcggctcgg ggtctgccag cagaacgccg ccagtgccga ggaaccagac
     9481 gagcccggtg acgatggtca ttccaatctt cgttgaagtc ttcatctgct cctccctacg
     9541 tccgatccgc cttggaccag ttgatgatcg cgttttcgtc gatagcgttc tcgaggaacg
     9601 ccgcgaacat gccgcgcagc atcccatcga gctcggcaag cagctccgag gtctccatcg
     9661 cggtttcttc gggggtctcg ccgaggatcg tcggcagctt cacgcccttc cactgaagcg
     9721 cggtgttgag cgtgcccatc cagagcttgt cgccgatgaa gatgcacagc ggcagttcgc
     9781 ggacgctgta gccttccgcg atcgcgcgcc gcgcctggtc cgcgtcccca ttgaacgctg
     9841 ccaccgcctc gccgtgcccg agcaagaact tttcgcccat ctgcacgacg aatcgatcga
     9901 gacccggcaa catccattcc ccgttgttct ggctgctctt gtagaagagc caagtgagga
     9961 acttcggcgc ggtgtggaga tcggccttgt cgttccaggc gagcgtgccc tcgatgtcgg
    10021 tgggaacctc gaaggtctgg ccgaagacga gcaggaagct gtcgatgacg ctctggctct
    10081 gggtgtagag gtagacgcgg tttcgcgcga gatcccagag caccggaacc gtcgagatct
    10141 tcgacggaat cttcttccga agatccatct tcagaacatc ccggatctcg atcttgcgga
    10201 acttgggaag cggctccttc agttccgctt cgagttcggc cacgcgctgg cgcaccagcc
    10261 gcttcagatc cttgccggcc ggcttcagcg tctcgatgcg gaggtccatc aggacgatgt
    10321 tgttgtagtt ccaggcgggg tgcagattca gctcatcccc atgctggcac cagccaaccg
    10381 cgcgctcggt gttggtctcc ggctgaatcg gcacggcagc atgagccgcg atccggcgct
    10441 cgacgttctt gggcagcgtt ccctttgcag cgaaccgggt caaattcatg gtctctcctg
    10501 ttgtgttttg ctagtgacta ttcagagcta gcagtatatc tttttgcagt caagctagtt
    10561 tctagctgga ccgattgata caagaggctc aatgtccgca atcgacctgc gtcgacagct
    10621 aaccgacgtg caacaagtcc tcgacggctg tggctaccga ggcgaaatcg tcaaggaagg
    10681 gcgcggcgtg aagatccgct gcccttggca cgccgacgac gggcgcccga atcttcagat
    10741 ctcgatgcgc agcgatggca cgctcggggt gaagtgtttc tcctgccaga agggcggcga
    10801 catccttcac ctgatcggcg agctcactgg caagggcgat ccgaaaacca acttcaaggc
    10861 ggtcgcacag gaagccgagc gcatcgccgg cacccggatg acctcagcgc cagcgcccag
    10921 gacttcgagt actgcggata actttccaga caagctcgaa gtcgcgaagt tttggaacct
    10981 caccgtcgat ctgcaaacca acgccgatct ctgcaaccgc ctcaccgcac gccacatcga
    11041 tccgcagctc atcaaagagc ttgcgcgcgc cctgcccgac ctatcgaaga atccccgctg
    11101 gggatgggtt cgcaatcctg accccagcgg cgatccgctg acgtgggcaa aagctggcta
    11161 ccggctgatc gtgcccatgt acgatgaggc gggcgagctg gtttctgttc acgcgcgccg
    11221 ctttctcggt gcagacgaca aacagaaagg cacatccccc agccgctgcc ggatcgcggg
    11281 ctcgatgatg ctgtcgccgg ccgcactgca aagcgcggtc tgggacgatg tgatctttgt
    11341 cgagggtgtt ccgaactatt tgatccttgc gtcgctttat ccaacgcgcc ccgtcgtcgg
    11401 agtgatttcg ggttcgatca agggctgcac cttcccccgg atcccacgcg gcgccagcgt
    11461 cactgtgtgg atggatgcgg atgcgcagcg gaatcgagcc gggcaagaaa tcgagggagc
    11521 tggccagatc tacacgaacc acgcgcttgc cgcgctgcgc ggtcggcgca tccgggtcgt
    11581 aaaactcgcg atccccgaag ggaaaaagaa agctcccgac gccaacgaca tctacatcgc
    11641 gggcggcggc cagatgatca gcgatgtgct gtcgcaggcg gagacaaacg cggccccgct
    11701 agaccccaag gccgaggcga tgtggaatcg cggcgctggg gtctatggga tgcagcggcc
    11761 cccgctcaac atggaccgtc tcgaagatcg cgagatcgtg cggctcatcg acagcaaact
    11821 cgagacacgg cgctggaacg agttcgggga gctcataggt attggcgagg gaccaggcgg
    11881 ctggttcgcg aatcagcagg gcctgcactt cctgcgcacc ggcgaagcgc caccggccgg
    11941 catcgtggtg acgcagggct tcgaaaattt tctggctctg tcgcagctct gggacgatga
    12001 cgcggtggcg atggagcccg cgattctggc gggcctgccg ccaaagacta catcgcgcct
    12061 tgtgctctgg ccctcgcagc cctgcgagca agaccacgaa gtggtacggg cctccgagcc
    12121 gcgggtcgca gcggagcgcg ggaacaaggc gatgttcgcg tgcctgtccg cggcgcagcc
    12181 agttccgacc gtgctgccga ttccgccgtc ggaaaaagag gcgaagcagt tcatgcggac
    12241 cgacctcggg aacgcggaac ggctcgcgca cctctatggc gacgatatcc actacgtcaa
    12301 agactgggat agcttcctcg tctgggatgg caagcgctgg gcgcgcgatc ggacgttcga
    12361 ggtcgacagg cgcgccgcct atgtggtgcg ccagctttac gacggagacg agaaggatcg
    12421 cgcctgggcg cacgactccg agagcgcggg caagctctcc gcgatggtca gcatcgtccg
    12481 aaagctgccc ggcgtgccga tccttcacga gcagctcgac gccgatctgt acctgctcag
    12541 cgttgccaac ggcacgctcg atctgcgcac cggggaactg ctcacccaca gccgcgacca
    12601 cctgcacaca aagcgcgccg atgtcatcta cgaccggaag gcgaagtgtc cgaagtggca
    12661 cgagttttta cgcaccatct tcgacggaaa cacgaacctc atctcgtaca tccagcgcgc
    12721 cgccggctac tcactgaccg gcgatgtggg tgagcacgtc ctgttcatgt tgtatggcac
    12781 tggccgcaac ggcaagagca cgttcttgat gacgctacag tcgctgctcg ccgactactc
    12841 gaaagcaatc agctctgaag tgctgatgag cacggggcgg aaactcgacg ccggccagca
    12901 gtccgcgatc gcgagcttgc gcggagcgcg ctttgtcgtg tcctcggaaa cggaggaagg
    12961 agccaagttt tcagagtcag tagcaaagca gctcaccaag ggcgacatca tccaggcgaa
    13021 gttcatgggc cgcgacgtgt tcgatttcac gccgacgcac aagctctggc tcgccacaaa
    13081 ccacaggccc aagacccgcg gcaaagacga cggcctgtgg accatgctga tcccggtgcc
    13141 gttcaccgtc acgatcccca tcgagaagcg agatcgcacg ctgcccgcgc agctcctcgc
    13201 cgagcgctcc ggcattctca actgggcgct cgacggctgc ctcgactggc aggtaaaccg
    13261 cctcgggcgc cccgctgaga tcgatgccgc ggtgaacagc taccggaaag agatggatca
    13321 gattggaccc ttcatagagc agtgctgcat cgtgggcgcc gatcatcagg tgaagatctc
    13381 ggcgctgcgt aaggcatacg agagctggtg cagcgaaacc agcaaggaac cgctcagcgt
    13441 ccgcaccttc ggatcgattc tcgaagagaa attccccgcg gacagcaccg gcgactatcg
    13501 gttccgccgc ggtctcatgc tccggggcga atgggtagcg accaatgagc gaaactgatc
    13561 tcgcgcggct cgcgctgcgg cagctcgaag agatgggctt cgactgctat caagaggtcg
    13621 cgcacggcgc ccaccgcgcc gatatcgtcg gcgtccggcg cggcatcgtt tgcatagtgg
    13681 agtgcaagac cgcactcagc ttcgatgtgg tggcgcaggc cgacgactgg ttgccggttg
    13741 cgcactggcg attcatcgcg gtgccaatgg cgcgctacgc aggccgtggg cgcgagctcg
    13801 cctatgcgat ctgcgagcaa cgcgggatcg gcgtcctcga gatctccgag cagcatgatc
    13861 gggtttggat ccgccggtac cccgcgctca accgggccgc gaagccccag cgcctgctcg
    13921 atgcgctgcg ccccgaacac aagacctatg ccgccgccgg cagctccggt agctattggt
    13981 ccccgtggaa gtcgagcgtg cagagtctgg ttctcgaggt caaccgaaag ccagggcgcc
    14041 cgctgcgcga tgtgatcgcc cacatcaaac accactgggt gagcaataag agcgcggtga
    14101 gctgcgtcag ccggcagatc gccgatggcg cgattgaagg gctgcggctt gagaaaaaag
    14161 ggcgagcggt cttggttttt ccgacggcga aataaatatt tatcggaaga taaaaaaagt
    14221 attgcttccg ttttgaaaat gaatagactg ggtttcagaa agcgagacga acccagatga
    14281 ctgccgccat caaagccacg cgcgccgccg gatacaagct gcttggcaag ctcgccgagg
    14341 tcatgaacat caccgcgcac ctcggccacc tcacggaata cccgctggtc gtggccaatc
    14401 tccaaaagct gcgcggcatc gtgatcagcc acatcagcga catcggagat cccgagcgct
    14461 ggcacacctt cgaagatgtg ccgaacctcg atcggcttca cgtcgtcgac agcatcgaag
    14521 agttgaatcg ccacccggcg aagcacgaag ccctgcttgc gcagctcgcg gccgaagctg
    14581 ctgctgccaa tgagatcctt tttggtaagg gagcggcgta aatgaaaacc gtcttcacca
    14641 tcgtctacga cgcgcccgaa ctcggccccg gtcccaacgc cgacgacacc cacaccaagc
    14701 gcttcacgaa taagaagcag gccgagacct tcgcggccgg caagacctac tacggccagc
    14761 ccgtgacggt gcaggaagat aacaacgtgc cgccgaagct gttccagcgg tggaagcgcg
    14821 aagggaaaat cggatgaaag tcctagcgct gccataccgc ctcgaagcgg ttcccgatcc
    14881 cgtcctcggt aagattggga tcgcggttgg aaccttcgca agcgccaaag atgcagcgaa
    14941 cttctttcag cgctatccga acgcggcaac cccgtgctgg cagcggatgg aagtgatcga
    15001 cggcgaactt gtgcaggtgc ggtgaaatta tgaacctcgg ccacatcaac ttccaccgcc
    15061 tcaccgatag cgacctcgcc tcgatctggg ctgcgatcga gacgcagccg gcgcggcccg
    15121 aagcgcttga gatgatcgac gcgccgcccc gctgcaccat gaagggcttg ctcaagcggc
    15181 tggcgcaggc cgagccgcag cacgaggcca aggcgccgct tgtcgtctcg tgcggcatcg
    15241 gcgtcgattc cgtggcgatg ctggttggcc tgtggcgccg tggcatccgg ccggatctga
    15301 ttctgtttgc cgaccccgga tcggagtggc ccgagaccta tctctatgtg ctggtgctgg
    15361 cggcatggct cgccgaagtc gggtttccgc cgcttaccat cgtgcgccga gaccaggggc
    15421 gcaccagcta caccacgctc gaaggtaact gcttggtcaa ccgcacgctg ccctcaatgg
    15481 ccttcgggta cggcggctgc tcgcagaagt ggaagcgcga gcccatggac aagctcgtgg
    15541 gccggtggta cccgcgctgc gcgcgctacg agctggtgtc aagacccagc gcgcgatcgg
    15601 ctacgacgct ggccccaaag actgccggcg cggcaacatc aaagactgcg cccgctacca
    15661 ctacgtttac ttcctgcgcg agtggggctg ggaccgcgac cgctgcgaag ctgagatcat
    15721 cgccgccggc ctgccgctgc cgcgcaagag cgcatgcttc ttctgcccag cgatgaaggc
    15781 gtgggagttg cgagacctaa tccggctgca cccagccctt gccgatcgcg tgatcgccat
    15841 cgagtccggc gcagccaagg cgctgatcaa agtcgagggc ctgtggcgca aggccaccaa
    15901 agagaagccg ggcagcatga cggcgtttat caacgcggag cgagcaaatt gaccaaagaa
    15961 ccaaacattc tctcggtgcg agtcggcggc gcaggcggaa ccgtctatca caacgtccgg
    16021 ctacatggca gcggcggtct atgtccagat cgagaggccg cgatcaagct actcgagaag
    16081 cggcagaagc gcggtgaaaa gatcatgctg catcacaact acagcgcgca gggcagcgac
    16141 aagacccatc acgatgcggt ggatgtagag attctgccga aagagtagat ctcccttggt
    16201 gcggccccta cgggggcgcg acggtagcca gtccgtcgat cctgccgcgg tggcaggttg
    16261 gcccggagaa tcccatgact cagatcgctg ttgtctaccc gacctctgaa gacgcgattg
    16321 ccacgctccg cgagcgctat accggcctca gcgccgacac acccaagggc tacgaagaag
    16381 tgcgcgtggc gattggcgag ctgcgcaagc tgcgggtcgc ggtggaagag cgccggaaag
    16441 aactcaagaa agactcgctc gaatatgggc gccgcgtcga ttccgtggcc aaggcactga
    16501 ccgagcagct cgaagcaatc gagaacccgc tcaagctcct gaaagaagct gccgacgaag
    16561 aaaaggcgcg cgcgaaggcg gagaaggaag ccgcggagcg gcgcgagttc gaagagaagc
    16621 tgcgggccga gcaggaagcc cgcgagcagc gcctcgccga gatccgcgcc caagaggaag
    16681 ccaggatccg cgcagaggcc gagcagcttg cgcaggagcg cgccgccttc gaagctgcgg
    16741 agcgggagcg cgcagcggcc atggaagccc agctccttga agcggagcgg caggaagcag
    16801 agcgccagcg ccttgctcag atcgagcgcg agcagattga ggcggagcgg cagcggatcg
    16861 ccgaggaagc cgccgagatg cagcgccagc aggaagccat cgttgcggag cggcagcgcc
    16921 tggccgatga gcaggccgct gccgaagccc gcgcgcaaga ggagctgcgc cggcagcagg
    16981 aagagctggc ggccgagcgg gctgagatcg agcggcagcg ggcggaactc gccaaacagg
    17041 ccgaggaaga agcggcgcag cgggagctgg aacggcagcg gcgcgccgct caggaagaag
    17101 cagatcggca ggcggcagcc cagctcgccg agcagcagga gcgcgagcgc gtggcgcagg
    17161 agcaggccca gcgcgaagcg gccgaagcgg ccgaagcggc cgaagctgcg gcgccccaag
    17221 cggtgccggt tcccatcgac aacgacgacg agacgttctc gatcgctgac ctcggtctcg
    17281 ggtcgagctc gacgagcgcg aagatctgcg aacacgagaa gttcaaaacc tcggtcgatg
    17341 tcacccgcct gaccagcggc ggtgatagcg agccgacctc gtacaccgcc gagattcgga
    17401 tccactgcgc ggcctgcggc gtccccatga agttcttggg tctgcaaggc ggcagccacc
    17461 ccgagcgccc gaccgtcagc ttcgatgggc tcgagctgcg ctgccccgtc attccgagca
    17521 gcgacaaggc cgaagggcag gcgtaaccat ggcgtcccgc gaaggtcggt tggtcgccgc
    17581 cgcgccggcc gagctgcggc gccaagtggt ggccactggt gacaccgagc tcttcggcgg
    17641 cttcgcaatc gaccctttcc gggtgcccac caccgacgaa gagatcgcgc acaagcgcaa
    17701 cgcgatggcc tttatcgagg ggaactatcc gctgagcatc ggcgattgcg aggtggtggg
    17761 catctcaggc ggctgcggcg aaggctgccc aatcctcaag cgcggcgcgt gcgagaccca
    17821 ggccgagatg gaacacgcac tcgcgcagct cgacctcaac cgcctgagcc gtgcatggct
    17881 gcgggactgc ctgcaagccg cgatggagat gcgatgaaaa ccaccaccgc agccacccaa
    17941 gcatccgcgc aggcgcggct cgagcgcgaa ctctgcgaac cgcctacaga tcctgcgccg
    18001 ggggcagccg gtgccggcgt gcgcatccgc acggcgcggc tgctccgggg gatcagccag
    18061 gcgcagctcg gggaagcggc cagagtaagc gagcccacga tcatcggaat cgagcagggg
    18121 cggagctggc gcggtgacgt gctcgagcgg atcgccaagg cgctggacat gagggccggc
    18181 gagctcgcgg ggtggctgtg attctccgcg tcggcatccc gaagcccaac gggtcgatga
    18241 cgcgcgagct gcaccgccgc cggcagcccg tactgatctc ggcgggcgcg ctctgggatc
    18301 cgaggacgga ggcgtttcgc tgctcgggcc tgctgctgct tcggatggat tgcgcgcttg
    18361 attcggccgg ctttgtccgg gggctgtctg gctatccctg gtccgtcgag caatatgtcg
    18421 agctcgcggc gggatcctcg tgggcatggt gggcgcagat ggatctgccc tgcgaagccg
    18481 cgatcgcgcc ggaccgcgag accgtggtgc gccgcgtcat cgaaagcgcc gcgctgtttc
    18541 gccgctgtgc cgccgccgcc gaaacggccc ggcaggcttc cgcctttctg cgcgagacct
    18601 tcgcggatcc gatgccagtg ctacaggggc ggcggctcga ggattaccag cgctcgatcg
    18661 agcttctcag cccgctgcct gcgctggtcg gggtcggatc gatgtgtacc cgccccatcc
    18721 acggacccga gggtctggtg ccaattgtag agggcctgca ccagcttctt cctgcctcgg
    18781 tgcagcttca tctctttggt gtgaaaggcc cagcactggc ggcgctcgag cgctttggcg
    18841 accggatcct gtcggttgac tcgatggcct atgacatcca cgcgcgcaaa accgcgcacc
    18901 gcgagcaggt ttcgaacacg gtggcgcgac gcaccagcgt gatgctcgac tggatcagta
    18961 agcaggggcc gtgccagcga tgatcattcc gtccgcggtg cctccataat cacgcaggcg
    19021 tagccaggga agttctgtcg aaacgcagcg atgtcagcag cggcagcggc gagagtgctc
    19081 cgtcgatagt ccatgtcgcg aatatcgggc gtgccgcttt tcactctctc agtgcagcgg
    19141 ccgaagtatt cgacggtggg cgtggtggtg ggtgctgtcg cgcagccgct gaggaacaaa
    19201 gcgaaaaaga gtctggtcat atgaagccct acgcttccgc gcaggacatc tgaccgaact
    19261 attttcgcgc gtcgttcgcg tgggtgccgg tgctggattc aacgagctgg ccctgcacca
    19321 cagcggcgcg gccctcgagc aagtccgcct tctcagcgct ggtcaagtgc cggccgagct
    19381 gggcctcggc ggcatcgatc tctgggcagc ggtctgggat ctttctatcc gacatggcgc
    19441 acctcggcac ggagtctgaa ttcagcgtcg accgacaact ctgccgcata gcccgagatc
    19501 cgctgcaaaa actcagtctg gtgctcgagc gagacgaggt cgagcagcgc aacgaaggcc
    19561 ggcgcttctt tatccccgag atgatctcgc gcagcagaga tcgcgggcca gaggcgggtg
    19621 agttcttcgc agcaggcttc cccggcgatg cgcagcactt cctcgagctc ggtctgggga
    19681 tagagcagca gccggggcct gtggtcgaag tcgatcgcca tctggacatg cagcgagtcg
    19741 tgcaggcagt gccggcgcgc ggtctgccgc ccccactccg cccaggtttc ggcatccttc
    19801 ggccgctccg ccatcttccg cagccagcgc tcggcagcgg catcgacggg gtttccctgc
    19861 cgcgcgctct gcaccgaagc aacatagggc tggatgtagc ggccccacgc ctgcttgact
    19921 acttgcccag cgtccacggg atgatctcca tcctgctgta cttggtgccg acgcagcgga
    19981 cgacaagcag ccgctctttc gacgtgatgc cgtggtagat gcggcagatg atctggtcgg
    20041 agaggatccg gcgcaacgcg ctgagatcga tgtcgctctc tacctgcgcc cgcgtgccgc
    20101 tcggcagtag ctcgcggagc gtcgtccagg ccgacagcgg ctcgggaacg cccatcttcc
    20161 agtggtcttt gctcatgagc agaaacgcga cgcccttatc gaccagcgtc acctgctcgg
    20221 cggatagttc cgcgtcgccc ttcggatcga cgggttcctg acggttgagt ggccagcgat
    20281 cgtcgggcag cttttcgaac tggggcgcct tcggcgattc ccccgtcgga gtcgtggcgg
    20341 gaaggctcgt cgacttggtc tcggcgggct tttcccccgc gggcttggtc tcggccgccg
    20401 tcgtagtcga cgaggtttcc aaagaggttg cagtggtcgt ggcggaagtt gacgcggcag
    20461 gcgtcttttc cgtcgctggg gtcggttttt ggtctttgga catgaccgat atagttgcta
    20521 ccggcaagat tcaaaatcaa cagaaatcgg gggcagattg gagatttccg acgggggaca
    20581 aggggggaga gagggggacg gctcggtctc cgcggatttt tttggcatcg aggcaacggg
    20641 cgccccgctg tcaagcggaa gatttggagc atcggcgttt agcgagagcg cccacaaaaa
    20701 atgagcggcc cgcagaattt gtagaatttt tcggcctgtg gtttgcaagg cgtttgtctc
    20761 tctttctttt ttctgtatga aaccagtagt tctgacacat atcgtgtcag actgacacaa
    20821 gaaagaaatc ttatgtcagc cgtcgctgac tgacatgact tacacaaatt acatgagatc
    20881 ttaaatctcg cgcttacgcg cgcgcatttg cgcgcacgac ccggattcga tctga
//
LOCUS       DAOQNN010000013        20685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_917_length_20685_cov_9.852981, whole genome shotgun sequence.
ACCESSION   DAOQNN010000013 DAOQNN010000000
VERSION     DAOQNN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 20685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20685
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_917_length_20685_cov_9.852981"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PKV97_01905"
     CDS             complement(<1..472)
                     /locus_tag="PKV97_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNB04657.1"
                     /translation="MQTLNVALGERAYPIHIGRGLLGRADLILPHLKTQRVAIVTNEV
                     VGPLYLERLRAGLEDAGVRTAAVVLPDGEAHKDWETLARIFDMLLAERCERSTTLVAL
                     GGGVVGDMGGFAAACYQRGMPFIQVPTTLLSQVDSSVGGKTAINHPLGKNMIGAF"
     gene            complement(460..990)
                     /locus_tag="PKV97_01910"
     CDS             complement(460..990)
                     /locus_tag="PKV97_01910"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNB04658.1"
                     /translation="MSCLILIGMMGAGKTTVGKELARRRRMRFADCDQAIVARTGVSI
                     PTIFELEGEAGFRRRETQMIDELTREPDLVLATGGGVVVTPLNRELMRERGIVIYLDV
                     PTQVLFERTRHDRNRPLLQVENPRQRIEELHRARDPLYRETAHIILAGGRGNPCNMVR
                     QIEEALARYKADTCRP"
     gene            complement(1079..>2548)
                     /locus_tag="PKV97_01915"
     CDS             complement(1079..>2548)
                     /locus_tag="PKV97_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ family protein"
                     /protein_id="HNB04659.1"
                     /translation="LRRRSDVTDFATPVTSMTAQQVGDRVRLTVTPNGLWEHNAYQSD
                     NRFVLEVRRVAEDPTKLGGGRSDYKGDKLSLNFQNVDVRSVLQVIADFTDFNIITSDS
                     VQGNLTLRLKDVPWDQALDIILQAKGLDMRKNGNVIWIAPGDELAAREKLQLEAKAQI
                     VDLEPLQTESFQINYHKAKEIFDFLKSKDQTMLSKRGSVVVDERSNKVFVTDVATRLQ
                     ALRRLVQEIDVAPRQVLIEARIVEANKTFARDLGVRLGFGSKGVASLDGARLGFGSSS
                     LTGGGSNNPNTFTPTVAQSGTGFMPVGYGSLGLSLFNSRATRFLNLELQALETDGRGR
                     VVSSPRVLTANQVEASIEQGTEIPYQEASSSGATSVSFKKAVLSLKVKPQITPDGRLQ
                     LSIEVNKDRPLYEQTLLGVPPIETKNVKSEVLIENGGTVVIGGIYEEEESTGEDKVPV
                     LGDVPVLGHLFKSQSKVSNRKELLVFITPRIVSDALTLR"
     assembly_gap    2549..2648
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2673..3197)
                     /locus_tag="PKV97_01920"
     CDS             complement(2673..3197)
                     /locus_tag="PKV97_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HNB04660.1"
                     /translation="MMNRAVLIGACVLLAGCAGGGQDDLEAWMGEQAKTMRGAVRPLP
                     ELRQVAVVAYAGTQGDDPFRSARMEPERRASASALRPDLDRRREPLEAFPLESLQMVG
                     VLMQGNNTHALVKAGTVLHQVKAGNYMGQNFGVITLVTENEIKLRELVEDVYGDWVER
                     TSTLTLQERQEAGK"
     gene            complement(3194..3868)
                     /locus_tag="PKV97_01925"
     CDS             complement(3194..3868)
                     /locus_tag="PKV97_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNB04661.1"
                     /translation="MSFDLGALRDGIDFRRLAEDFRGLDPQDPGMWPAAPRVVVAVCL
                     LLLTVAGFWWFDWQGQVETLEARQTEEVQLRESWVTKKRQAVNLEEHRRQLAEIDRQF
                     GALLKQLPNRAEMDSLLSDINQAGLGRGLQFELFKPGADVIKEFYAEMPIDVRVTGVY
                     HDLGEFVADLASMPRVVTLGDVGIEADKDGRLKLQAKAVTYRYLDEEELAQQRKAAQA
                     AKQGNK"
     gene            complement(3865..4470)
                     /locus_tag="PKV97_01930"
     CDS             complement(3865..4470)
                     /locus_tag="PKV97_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNB04662.1"
                     /translation="MIRINLLPHRIAKRRQRRRQFYALAGSMVLLGALIGFGVHMVYT
                     AHIEDQEARNAFLKEEIVRLDREIAEIRRLREQIDAMLARKQVIESLQGTRAETVHLF
                     NELAQRLPDGVYLKSVKQTGRQVRVVGLAQSNARVSHFMRNVEASVFLEQPRLIEAKA
                     VTQDNRRLSEFSLDIAIRPPVQAEPEGAAGAAKAAAKGTQK"
     gene            complement(4467..5543)
                     /locus_tag="PKV97_01935"
     CDS             complement(4467..5543)
                     /locus_tag="PKV97_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNB04663.1"
                     /translation="MIDLPFFSPSARQLVGLDISSSSVKLVELSAGEKDAYKVERYAV
                     ETLPRDAVVDGNIANLDAVSESVRRALRRFGSGVRNVALALPSSSVITKKIILPEGLR
                     ETEMEFAVEAEANQYIPFALEEVNLDFQRIGPAPGSPGDVEVLIAASRKDKVEDRVAV
                     AQAAGLKAVIVDVESLATESALELVCRQLPRGGADQVIALVDIGASVMNVTMFRNGHS
                     IYSREQAFGGSQLTQDIARQYGMSFEEAESAKRAGSLPDSYPRDLMRPFMDSLALEVS
                     RALQFFFTSTPFNQVDHVVLAGGCAVLPGLGDVVAARTQVDTLIANPFASMTLSGKVR
                     PKALLADAPALMVACGLALRRFDA"
     gene            5731..8154
                     /locus_tag="PKV97_01940"
     CDS             5731..8154
                     /locus_tag="PKV97_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNB04664.1"
                     /translation="MRWVLYPIAALLALAILGIATLAAMAFFAWPNLPALDTLTDYRP
                     RVPLRVYTADGHLIGEFGEERRDVVRIAEVPSHLKHAILAAEDERFYEHRGIDPLGLA
                     RAALANLSSGGRGQGASTITMQVARNFFLSREKTYNRKLYEILLSLKIEQNLGKDQIL
                     ELYINQIFLGQRAYGFAAAARAYYGKTLEQLTLPEAAMLAGLPKAPSAFNPIVNPARA
                     AVRQQYVLRRMLEAGFIDDAQYQHALAFATPKQSQRDIAAARRVQDIVVPGDYVAEMA
                     RQIAVEQFGEQAYELGIRIITTVTRKDQEAAYAALRRGVLDYDRRHGYRGPEAFVELP
                     SGQVPDEQLDEALGETNDHDDLLAALVLRASPKEVLAYRRGQEIRITGKGLSFVAPML
                     SDKAPQARRVRRGAIVRVRNTGKDGWELAQLPEVQAALVAIDSHSGAVRALIGGFDFH
                     RNKFNHVTQAVRQPGSSFKPFIFSAALDRQFGPGSVLEDNPLYYPAGVTGSKAWEPKN
                     YDGRYAGPMTLRDGLARSKNMISIRLLEKITPDYARDYLARFGFDPAQNPPYLTMALG
                     AGAATPWQMAAGYAVFANGGYRVDPFVVKEMYDGSGRLIARTDPPVAGQSAPRVIDAR
                     NAWLMDSMMRDVVQRGTATRARSLKREDIAGKTGTTNDYVDAWFCGYNPDLVAVSWVG
                     HSQPRNLGKGETGGAAALPIWINYMASALEGAPQVELPRPDGLMQVQNGLGTSQDWQY
                     AEYAPPAPPPVPDWLRDMFANDSAPAMEAESEAIPPGPAPLILPPPSPARAQAPAPVE
                     ERRPMPIRP"
     gene            complement(8289..8615)
                     /gene="cyaY"
                     /locus_tag="PKV97_01945"
     CDS             complement(8289..8615)
                     /gene="cyaY"
                     /locus_tag="PKV97_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HNB04665.1"
                     /translation="MEESVFNALAEAELARIETALEDCGAELDVEPKPGGILEIEFDN
                     GSKMIINRHTAAREIWVAAKSGGFHFRPLDGGWVNTRDGRELWAMLAALVGEQSGEAV
                     RLLRPN"
     gene            8703..8870
                     /locus_tag="PKV97_01950"
     CDS             8703..8870
                     /locus_tag="PKV97_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNB04666.1"
                     /translation="MQFPAIALACALLTACGIKGPLYLPAPGTASAPPAGADHSKPVV
                     PKDLIKESPLE"
     gene            8867..10138
                     /gene="lysA"
                     /locus_tag="PKV97_01955"
     CDS             8867..10138
                     /gene="lysA"
                     /locus_tag="PKV97_01955"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNB04667.1"
                     /translation="MNAPASRWPAPTLSARDGALWLEELPLAEIAARFGTPTYVYSRA
                     ALEAAFAAWKDALAGRRALVCYAVKANSNLGILSVFARLGAGFDIVSGGELARVLAAG
                     GRADKVVFSGVGKTRAEMRQALQAGIRCFNVESAAELEHLDAVAGELGVRAPVSLRVN
                     PDVDPKTHPYISTGLKGNKFGVAFADAPALYRRAAALPNLRITGVACHIGSQLLDRAP
                     IAEAAQKLRGLVEGLAADGIALEHIDLGGGLGIRYRDETPPPVAEYLAPLLEVFADRE
                     EELCFEPGRSLVGNAGLLLTRIEYLKPGETKNFAIVDAAMNDLARPALYDAWHEVVEV
                     TPRALAPTTYDVVGPICESGDFLARARSLAVAEGDLLAILSAGAYGMTMASNYNTRPR
                     AAEILIDGASAHVVRERETVEELFARERTPG"
     gene            complement(10222..10572)
                     /locus_tag="PKV97_01960"
     CDS             complement(10222..10572)
                     /locus_tag="PKV97_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04668.1"
                     /translation="MSGFGHYTRTADELEREIVKRGIAIGIDWGDASRMRELAHRALT
                     CTPACMMKLLRSPVRQDKLTGELFALSELMLQNMRQSAEIGFETHGGPAWKAFGRALN
                     EEYDAGARPPVASA"
     gene            10716..12149
                     /gene="dacB"
                     /locus_tag="PKV97_01965"
     CDS             10716..12149
                     /gene="dacB"
                     /locus_tag="PKV97_01965"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNB04669.1"
                     /translation="MKNPSLCVLFIAGLLLAPITHAGVAGLPPGVRLALDSARIPAEA
                     VSLWTRPVDHATPTLAINATAPMNPASVMKLVTAFSALERLGPAHTWTTRIARDGPIR
                     AGVLEGNLYLVGGADPVLGYDRLWKVLRRLRALGIERVAGDIVLDASVLRLPPHDPDA
                     FDGRGVRPYNSGPYGLLLQYNTLLLGLTPGKAANAPVALAAEPPLAGLVIDNRVLTSD
                     ASCGSWYQDLQAVVEPGRRLVLSGSLPAACGPRTWSAAPLPPAEFGSALVAALWSEVG
                     GHLGGTVREGRLPTGAEELFSDDSDPLADVVRDMNKWSSNVIARQLLATLGRVSLRED
                     ATEDMVTAGARAAAATLAAAGIDTDGLVIENGSGLSRIERIRADSLGMLLLAAWQRPW
                     MPEFIAALPVAGVDGTARRRLGDSPARGQAHIKTGTLNGVRAMAGYLLDRNGQRHAVV
                     MMVNHPEAAASAAAQDALLEWLWSGGE"
     gene            complement(12323..12736)
                     /locus_tag="PKV97_01970"
     CDS             complement(12323..12736)
                     /locus_tag="PKV97_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04670.1"
                     /translation="MNSPYPAPLEAGQPSENMVTVTHLVYALHAFAVFSAVVGSATII
                     FSFVASLPSIAAVILNYWNQNAVRGTWLESHFRWQIRTFWFAVLWIAVSVLMVVTIIG
                     VPFALALLLIAGLWILYRVVRGWWNLMQRQTMPMA"
     gene            complement(12848..14338)
                     /gene="ubiD"
                     /locus_tag="PKV97_01975"
     CDS             complement(12848..14338)
                     /gene="ubiD"
                     /locus_tag="PKV97_01975"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HNB04671.1"
                     /translation="MKYDDLRDFLAQLEARGELRRIKTPVDTHLEMTEIADRVLRAGG
                     PALLFERPVTRGVAQAIPVLANLFGTPQRVAMGMGEEVADGDWSTPLREVGKLLAYLK
                     EPEPPKGLKDAWDKLPVLKQVLNMAPKEVRSAPCQQVVWSGDEVDLAKLPIQHCWPGD
                     AAPLITWGLVVTRGPHKKRQNLGIYRQQVIGRNRVIMRWLAHRGGAIDFLEHQRAHPG
                     EPFPVAVVLGCDPATILGAVTPVPDSLSEYQFAGLLRGAKTELVKCLGSDLQVPASAE
                     IVLEGAIHPGDMAPEGPYGDHTGYYNEVSDFPVFTIERITMRRDPIYHSTYTGKPPDE
                     PAMLGVALNEVFVPLLQKQFTEIVDFYLPPEGCSYRLAVVSIRKQYPGHAKRVMFGIW
                     SFLRQFMYTKFIIVVDEDVNIRDWKEVIWALTTRMDATRDTTLVDNTPIDYLDFASPV
                     AGLGSKMGLDATNKWPGETSREWGTPIVMDAAVKAKVDAMWGELGL"
     gene            complement(14426..15250)
                     /gene="pyrF"
                     /locus_tag="PKV97_01980"
     CDS             complement(14426..15250)
                     /gene="pyrF"
                     /locus_tag="PKV97_01980"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNB04672.1"
                     /translation="MHFMTALRAAWQQRDSLLCVGLDPDPARFPAHLQGRSDAILRFC
                     TQIVDATADLVCCFKPQIAYFAAHRAEDQLEALVEHIHARHPGTPVVLDAKRGDIGST
                     AEQYAIEAFERFKADAITVNPYMGRDSVDPYLAYPDKGVILLCRTSNPGGSDLQFLEV
                     DTSKGRMKLYEHVARTVAEDWNASGNCALVVGATFPAEIARVRALVGELPLLVPGIGA
                     QGGDIAATLAAGRTENGTGLMINSSRAVLYAGKGEDFAEAARRVAEETRDAINAYR"
     gene            complement(15298..16176)
                     /gene="ubiA"
                     /locus_tag="PKV97_01985"
     CDS             complement(15298..16176)
                     /gene="ubiA"
                     /locus_tag="PKV97_01985"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNB04673.1"
                     /translation="MTPAMSLSDKLPHYARLMRIDKPIGTLLLLWPTLWALWIAAAGW
                     PPLHILVIFIVGTFLMRSAGCVINDYADRDFDGHVERTRTRPLATGVVSPGEALALAA
                     ALSAAAFVLVLPLNTLTILLSVPALLLAGSYPFTKRFFAIPQAYLGIAFGFGIPMAFA
                     AVTGTVPATGWLMLLANIFWAVAYDTEYAMVDRPDDLKIGIKTSAITFGRHDVLAVML
                     CYAATFVLLTVVGALAGLGFAWYAGLVVACAIAGYHYTLIRARDRADCFKAFRHNNWV
                     GGAIFAGLFIDLLLRA"
     gene            complement(16173..16766)
                     /locus_tag="PKV97_01990"
     CDS             complement(16173..16766)
                     /locus_tag="PKV97_01990"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HNB04674.1"
                     /translation="MRPLTAFPLHTGPRHETWLAHPPRVLLPPALLPWLKDAGSLTAR
                     IRARCTRFEVRVLCQRLARVRRDEAWLLGLRVGELAWLREVLLVADGRPVVFARSLLP
                     RRNLRGAWNLFHGIGARPLGAALFSDPAIARLPLACRRLDGRDARYHRAAAALARQSR
                     APALPRALWARRSVFLLRGRALMVSEAFLPAILELRK"
     gene            complement(17044..19167)
                     /gene="recG"
                     /locus_tag="PKV97_01995"
     CDS             complement(17044..19167)
                     /gene="recG"
                     /locus_tag="PKV97_01995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNB04675.1"
                     /translation="MPADQRRARETCCPFPPSEKDRRLNATQPPRGWPGVGAQLAGRL
                     ARLDLRGPRDVLLHLPLRYEDETRLTPIARLRPGFPAQVEGEVLGSEVVLRPRRQLVA
                     RIADDSGTLVARWLNFYPSQQKPLAPGGRVRLFGEARGGFFGLEMVHPRLRAVAPGEA
                     LPDALTPVYPTTAGVGQITLRKLVAQALQSEPLDELLPAAWLHRLGLPGFAEALHALH
                     HPAPDADPFALEQREHPAWQRMKFEELLVQQLSLRRAWLARRAQAAVALPARALLTAA
                     LLESLPFRPTGAQARVGAEIAADLAAPHPMQRLLQGDVGSGKTLVAALAMLQAAENGW
                     QAAMMAPTEILAEQHWKKLSAWLEPLGIGVAWLSGSRRKRAREAELARLASGEVLLAV
                     GTHALIEDPVTLPRLALVVVDEQHRFGVRQRLALREKGEGGRRPHMLMMSATPIPRTL
                     AMTHYADLDVSVLDELPPGRTPIRTRLVSDVRRDEVVARVRAACLEGRQAYWVCPLIE
                     ESEALQLQTAQDTFATLVEALPELRVGLVHGRLRNEEKSATMAAFSAGALDLLVATTV
                     IEVGVDVPNASLMVIEHAERFGLAQLHQLRGRVGRGTAESVCILIYAQPLSQTGRERL
                     KVIYENTDGFAIAREDLRIRGPGEFVGARQSGVPLLRYADLELDAGLVEPARELAEHL
                     LAHDPAAANALMRRWLGGREALLRA"
     gene            complement(19262..19642)
                     /locus_tag="PKV97_02000"
     CDS             complement(19262..19642)
                     /locus_tag="PKV97_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNB04676.1"
                     /translation="MARQIISTPKAPAAIGPYSQAVKVGNQVFLSGQIGLDPVTMEMV
                     EGVEAQARRVFDNLAAVAEAAGGSFADVVKITIYLTDLANFGVVNEIMKSCFAEPYPA
                     RATVGVRELPRGALVEADLVLVLG"
     gene            complement(19724..20161)
                     /gene="fliW"
                     /locus_tag="PKV97_02005"
     CDS             complement(19724..20161)
                     /gene="fliW"
                     /locus_tag="PKV97_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNB04677.1"
                     /translation="MQIKSPLFGSAEIAEDKIIEFPKGLPGFEECRRFTLLHEEGREA
                     HVFLLQSVDEPDVAFSVTAPERLGVHLEFPLSDEEVAMLRLRTPEDAAVAVIVRKDEG
                     ESPAGTGLRANFMAPLVINTGERVGLQKLIDRLGCEITLRAGG"
     gene            complement(20236..20466)
                     /locus_tag="PKV97_02010"
     CDS             complement(20236..20466)
                     /locus_tag="PKV97_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB04678.1"
                     /translation="MAPAGSGYLVQLGVFAAPDNALRIYQQAAAAGQPAHIQSRVVLG
                     PFVDREAAERARKRLQAAGAGPGVLIPPERPR"
BASE COUNT         3198 a         7265 c         7070 g         3052 t
ORIGIN      
        1 agaaggcgcc gatcatgttc ttgccgagcg ggtggttgat cgcggtcttg ccgcccaccg
       61 aggagtcgac ctgcgacagc agcgtggtcg gcacctggat gaagggcatg ccgcgctggt
      121 agcaggccgc ggcgaagccg cccatgtcgc ccaccacccc gccgccgagc gcgaccaggg
      181 tggtggagcg ctcgcagcgc tcggcgagca gcatgtcgaa gatgcgggcg agcgtctccc
      241 agtccttgtg cgcctcgcca tcgggcagca ccaccgcggc ggtgcgcacg cccgcgtcct
      301 cgaggccggc gcgcaggcgt tcgaggtaga gcgggcccac cacctcgttg gtgacgatgg
      361 cgacgcgctg cgtcttcagg tgaggcagga tcaggtcggc gcgcccgagc aggccgcggc
      421 cgatgtggat gggataggcg cgctcgccga gcgccacgtt cagggtctgc atgtgtcggc
      481 cttgtagcgg gcgagcgcct cctcgatctg gcgcaccatg ttgcaggggt tgccgcggcc
      541 gcctgcgagg atgatgtggg cggtctcgcg atagaggggg tcgcgcgcgc ggtggagttc
      601 ctcgatgcgc tggcgcggat tttcgacctg cagcagcggg cggttgcggt cgtgacgggt
      661 gcgctcgaac agcacctgcg tcggcacgtc gaggtagatc acgatgccgc gctcgcgcat
      721 cagctcgcgg ttgagcggcg tgacgaccac cccgccgccg gtggcgagca cgaggtcggg
      781 ctcgcgggtg agctcgtcga tcatctgcgt ctcgcgacgg cggaatcccg cctcgccctc
      841 gagctcgaag atggtcggaa tgctgacgcc ggtgcgcgcc acgatcgcct ggtcgcaatc
      901 ggcgaagcgc atccggcgcc tgcgtgccag ttccttgccg accgtggtct tgccggcgcc
      961 catcatgccg atcagtatca gacagctcac gcgtgactcg ccggaaatcc tgatgaaaaa
     1021 gggcgggcat ccatgccgga tgcccgcccc cgggattgta tcagcagaag cgccggactc
     1081 agcgcagcgt gagcgcgtcg gagacgatgc gcggggtgat gaagacgagg agctccttgc
     1141 ggttcgacac cttgctctgc gacttgaaca gatggccgag caccggcacg tcgccgagca
     1201 ccggcacctt gtcctcgccg gtcgactcct cttcctcgta gatgccaccg atcacgacgg
     1261 tcccgccgtt ctcgatcagc acctcgctct tgacgttctt ggtctcgatc ggcggcacgc
     1321 cgagcagggt ctgctcgtag agcgggcggt ccttgttgac ctcgatcgac agctgcaggc
     1381 ggccgtcggg ggtgatctgc ggcttgacct tgagcgacag caccgccttc ttgaaggaca
     1441 cgctggtcgc gccggagctc gacgcctcct ggtaaggaat ctcggtgccc tgctcgatcg
     1501 aagcctcgac ctggttggcg gtcagcacgc gcgggctgga aaccacccgc ccacggccat
     1561 cggtctcgag agcctgcagc tcgaggttga ggaaacgggt ggctcggctg ttgaacaggc
     1621 tgagcccgag ggagccgtag ccgaccggca tgaaccccgt ccccgactgc gcgacggtcg
     1681 gcgtgaaggt gttgggattg ttgctccccc cccctgtcag cgacgacgaa ccaaagccga
     1741 ggcgtgcacc atccagcgag gcgacgccct tgctgccgaa accgagccgg accccgagat
     1801 cgcgcgcgaa ggtcttgttg gcctcgacga tgcgcgcctc gatcagcacc tggcgcggcg
     1861 ccacgtcgat ctcctgcacc agccggcgca aggcctgcaa gcgggtggcg acgtcggtca
     1921 cgaagacctt gttgctgcgc tcgtccacca ccacgctgcc acgcttcgag agcatggtct
     1981 ggtccttgct cttgaggaaa tcgaagatct ccttggcctt gtggtagttg atctggaagc
     2041 tctcggtctg cagcggctcg aggtcgacga tctgcgcctt ggcctcgagc tgcagcttct
     2101 cgcgcgcggc gagttcgtca cccggggcga tccagatgac gttgccgttc ttgcgcatgt
     2161 ccaggccctt ggcctgcagg atgatgtcga gcgcctggtc ccagggcacg tccttcaggc
     2221 gcagggtcag gttgccctgc accgagtcgg acgtgatgat attgaagtcg gtgaagtcgg
     2281 cgatgacctg cagcaccgag cgcacatcca cattctggaa gttcagcgag agcttgtcgc
     2341 ccttgtagtc gctgcgcccg ccgccgagct tggtcgggtc ctcggcgacg cgccgcacct
     2401 cgagcacgaa gcggttgtcg ctctggtagg cgttgtgctc ccacagcccg ttcggggtca
     2461 cggtcaggcg tacgcggtcg cccacctgct gggccgtcat cgaggtgacc ggggtggcga
     2521 agtcggtgac gtcggagcgg cggcgcagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnntc agcagcactc cgatgcggtg tctcattttc ctgcctcctg gcgctcctgc
     2701 aatgtcagcg tgctggtccg ttcaacccag tcgccataga catcctcgac cagttcgcgc
     2761 agcttgatct cgttctcggt gaccagagtg atcacgccga aattctgtcc catgtagttc
     2821 ccggccttca cctgatggag cacggtcccc gccttgacca gcgcatgggt gttgtttccc
     2881 tgcatcagca cgcccaccat ctgcagcgat tcgagcggga aggcctcgag cggctcgcgg
     2941 cggcgatcga ggtcggggcg cagcgcgctc gccgaggccc gccgctcggg ctccatgcgc
     3001 gcactgcgga agggatcgtc tccctgcgtt cccgcatagg cgacgaccgc gacctggcgc
     3061 aattcgggca ggggccgcac cgcgccgcgc atggtcttgg cctgctcgcc catccaggcc
     3121 tcgaggtcgt cctggccacc cccggcacag ccggcgagca gtacacaggc gccgatcaac
     3181 acggccctgt tcatcatttg ttcccctgct tcgcggcctg cgccgccttg cgttgctgcg
     3241 cgagctcttc ctcgtcgagg tagcggtagg tgaccgcctt ggcctgcagc ttgaggcgtc
     3301 catccttgtc ggcctcgatg ccgacgtcgc ccagggtcac cacccgcggc atgctggcga
     3361 ggtcggcgac gaactccccg agatcgtgat agacgccggt cacgcgcacg tcgatcggca
     3421 tctcggcgta gaactccttg atcacgtccg cgcccggctt gaagagctcg aactgcaggc
     3481 cacgcccgag gccggcctgg ttgatgtccg aaagcagcga atccatctcg gcgcggttgg
     3541 gcaactgctt gagcagggcg ccgaactggc gatcgatctc ggcgagctgg cggcggtgct
     3601 cctcgagatt gactgcctgg cgcttcttcg tcacccagct ctcgcgcagc tgcacctcct
     3661 cggtctggcg cgcctccagg gtctcgacct ggccctgcca gtcgaaccac cagaacccgg
     3721 cgaccgtaag caacagcaga cagaccgcga ccaccacgcg cggcgcggcg ggccacatgc
     3781 cgggatcctg cggatcaagg ccgcggaaat cctccgccag gcggcgaaag tcgatgccgt
     3841 cgcgcagcgc gcccagatcg aagctcattt ttgcgtcccc ttcgcggccg ccttcgcggc
     3901 gccggcggcc ccctcgggct ccgcctgcac cggcgggcgg atggcgatgt cgagggagaa
     3961 ctcgctcaag cggcggttgt cctgggtgac ggccttggcc tcgatcaacc ggggctgctc
     4021 caggaagacg gaggcctcga cgttgcgcat gaagtgcgac acccgcgcat tcgactgcgc
     4081 cagcccgacg accctcacct gcctgccggt ctgcttgacc gatttcagat atacgccgtc
     4141 gggcaggcgc tgggcgagct cgttgaacag atgcacggtt tcggcccggg tcccctgcag
     4201 cgactcgatc acctgcttgc gcgccagcat cgcatcgatc tgttcgcgca ggcggcggat
     4261 ctccgcgatt tcgcggtcga gccggacgat ctcctccttg aggaaggcgt tgcgcgcctc
     4321 ctggtcctcg atgtgtgcgg tgtaaaccat gtgcacgccg aagccgatca gcgcccccag
     4381 cagcaccatg ctgccggcga gcgcgtagaa ctgccgcctg cgctgcctgc gcttggcaat
     4441 ccggtgcggc agcagattga tgcggatcat gcgtcgaacc tccgcagcgc cagcccgcac
     4501 gcgaccatca gcgccggcgc gtcggcgagc agcgccttag ggcggacctt gccgctcaat
     4561 gtcatgctcg cgaagggatt ggcgatgagc gtatcgacct gcgtacgcgc cgcgacgacg
     4621 tcgccgagtc ccggcagcac cgcgcagccg cccgcaagca cgacgtggtc gacctggttg
     4681 aacggcgtgg aggtgaagaa gaactgcaag gcgcgcgaga cctcgagcgc gaggctgtcc
     4741 atgaaaggac gcatcaggtc gcgcgggtag ctgtcgggca gcgagccggc gcgcttggcg
     4801 gattccgctt cctcgaagct catgccgtac tggcgtgcga tgtcctgggt caactggctg
     4861 ccgccgaagg cctgctcgcg cgaatagatg gagtggccgt tgcggaacat cgtcacgttc
     4921 atgaccgagg cgccgatgtc gaccagcgcg atcacctggt cggctccgcc gcgcggcaac
     4981 tgccggcaca ccaactccag ggcggactcg gtggccagcg actccacatc gacgatcacc
     5041 gccttgagcc cggccgcctg cgccaccgcg acgcgatcct ccaccttgtc cttgcgcgag
     5101 gcggcgatca ggacctcgac atccccggga ctgccgggtg cggggccgat cctctggaag
     5161 tcgagattga cctcctcgag cgcgaaaggg atgtactgat tggcctcggc ctcgaccgcg
     5221 aactccatct cggtctcgcg caggccttcg gggagaatga tcttcttggt gatgaccgag
     5281 gacgacggca acgcgagcgc cacgttgcgc acgccggagc cgaagcggcg cagcgcgcgc
     5341 cgcacggatt cgctcaccgc gtcgaggttg gcgatgttgc catcgaccac cgcatcgcgc
     5401 ggcagggtct cgaccgcata gcgctccact ttgtaagcgt ccttttcccc ggccgatagc
     5461 tcgacgagct tcacggatga agacgagatg tcgagcccga cgagctggcg cgccgaaggg
     5521 ctgaaaaagg ggaggtcgat cacaaagaaa acccttgaat ttctttatgt tacgcgctat
     5581 tgctcagcaa cgctctagca tgctagcaac aagccattgg ggtgtaaagc gaaccccgtc
     5641 acggtcgaga ccggctcatc cacggacaca cgagccacgc gggcgcatcc cgtataatgc
     5701 cgctccgctc gaaaccatcc tggattgccc atgcgctggg tcctgtatcc gatcgcagcg
     5761 ctgctcgcgc tggccattct cggcatcgcc acgctcgcgg cgatggcgtt cttcgcctgg
     5821 cccaacctgc ccgcgctcga cacgctcacc gactaccggc cgcgtgttcc gctccgagta
     5881 tatacggccg atggccacct catcggcgag ttcggcgagg agcgccgtga cgtagttcgc
     5941 atcgcggagg ttccctccca cctcaagcac gccatcctcg ccgccgagga cgagcgcttc
     6001 tacgaacacc gcggcatcga cccgctcggc ctggcccgcg ccgcactcgc caacctgtcg
     6061 tccgggggcc gcggccaggg ggcctcgacg atcaccatgc aggtcgcgcg caatttcttc
     6121 ctctcccggg agaagaccta caaccgcaag ctctacgaga tcctgctctc gctcaagatc
     6181 gagcagaacc tcggcaagga ccagatcctc gagctctaca tcaaccagat cttcctcggc
     6241 cagcgcgcct acggcttcgc cgccgccgca cgcgcctact acgggaagac actcgaacag
     6301 ctcaccctgc ccgaagcggc gatgctcgcc ggccttccca aggccccctc ggccttcaac
     6361 ccgatcgtga accccgcgcg cgccgcagtg cggcagcaat acgtcctgcg ccgcatgctc
     6421 gaagccggct tcatcgacga cgcccagtat cagcatgcgc tcgccttcgc cactccgaag
     6481 caaagccagc gcgacatcgc cgcggcccgc cgcgtccagg acatcgtcgt cccgggagac
     6541 tacgtcgccg agatggcgcg ccagatcgcg gtggaacagt tcggcgagca ggcctacgag
     6601 ctcggcatcc gcatcatcac cacggtcacc cgcaaggacc aggaagcggc ctacgccgcg
     6661 ctgcgccgcg gcgtgctcga ctacgaccgc cgccacggct atcgcggccc cgaggccttc
     6721 gtcgagctgc cctccgggca ggtgcccgac gagcaactcg acgaggcact cggcgagacc
     6781 aacgaccacg acgacctgct tgccgcactg gtgctcaggg ccagcccgaa ggaggtgctc
     6841 gcctaccgcc gcggccagga gatccgcatc accggcaagg gcctgtcctt cgtcgcgccg
     6901 atgctctcgg acaaggcccc gcaagcccga cgcgtgcgcc gcggggcgat cgtgcgcgtg
     6961 cgcaataccg gcaaggatgg ttgggagctc gcacagctgc cggaagtgca ggccgcgctg
     7021 gtggcgatcg actcgcacag cggcgcggtg cgcgcgctga tcggcggctt cgacttccac
     7081 cgcaacaagt tcaaccatgt gacccaggcg gtgcgccaac ccggatcgag cttcaagccc
     7141 ttcatcttct cggccgcgct cgaccgccag ttcggtccgg gaagcgtgct cgaggacaac
     7201 ccgctctact acccggcggg ggtcacgggc agcaaggcct gggagccgaa gaactacgac
     7261 gggcgctatg cgggaccgat gaccctgcgc gacggcctgg cgcgctcgaa gaacatgatc
     7321 tcgatccggc tgctggagaa gatcaccccc gactacgcgc gcgactacct cgcccgcttc
     7381 ggcttcgacc ctgcgcagaa tcccccctac ctgaccatgg cgctcggcgc cggcgccgcc
     7441 accccctggc agatggcggc gggctacgcg gtgttcgcca acggcggcta ccgggtcgat
     7501 cccttcgtgg tcaaggaaat gtacgacggc agcggccgcc tgatcgcgcg taccgacccg
     7561 ccggtggcgg gccagagcgc gccgcgcgtg atcgatgcgc gcaacgcctg gctgatggac
     7621 tcgatgatgc gcgacgtggt ccagcgcggc accgcgacac gcgcgcgcag cctcaagcgc
     7681 gaggacatcg ccgggaagac cggaaccacc aacgactacg tcgacgcctg gttctgcggc
     7741 tacaacccgg acctcgtcgc ggtgagctgg gtcggccaca gccagccgcg caacctcggc
     7801 aagggcgaga ccggcggcgc ggccgcgctg ccgatctgga tcaactacat ggcctccgcg
     7861 ctcgaaggcg cgccgcaggt cgaactgccg cgccccgatg gcctgatgca ggtgcagaac
     7921 ggtctgggca ccagccagga ctggcagtat gccgaatacg ctccgcccgc gccgccgccg
     7981 gttccggact ggctgcgcga catgttcgcc aacgacagtg ccccggcgat ggaggccgaa
     8041 agcgaggcga tcccgcccgg cccggcaccg ctgatcctgc cgccgcccag cccggcgcgc
     8101 gcacaggcgc ccgccccggt cgaggaacgc cggccgatgc cgatccggcc ttgagcaggc
     8161 gggcgctcgc gggatggcga ctcgcgtcgg tcctgcaagg ggcggaaccc gcgatgacga
     8221 ctccgcggcc gccgtgcagg aacgacgcaa gtcgccatcg ggcgccccgg aaggagcgcc
     8281 ccttccgctc agttcggccg cagcagcctc accgcttcgc cggactgctc gcccaccagc
     8341 gcggcgagca tcgcccacag ctcgcgacca tcgcgggtgt tgacccagcc cccgtccagc
     8401 ggacggaagt ggaagccgcc cgacttcgcc gccacccaga tctcgcgcgc cgcggtgtgg
     8461 cggttgatga tcatcttgct gccgttgtcg aactcgatct cgaggatccc acccggcttg
     8521 ggctcgacgt cgagctcggc cccgcaatcc tccagggccg tctcgatgcg cgccagctcg
     8581 gcctcggcga gcgcattgaa cacagactct tccatgctga ttccttctgt tagcattccg
     8641 gttttttgcg aagaacggcg atgcgagtcc cacagcgagt cccgttgcga gtccccctac
     8701 gcgtgcaatt ccccgcgatc gcgctcgcct gcgcgctgct gacggcctgc ggcatcaagg
     8761 gcccgctcta tctgccggcg cccggcaccg cctcggcgcc cccggcgggc gccgatcata
     8821 gcaaacccgt cgtgccgaag gatctgatca aggaatcgcc gctcgaatga acgcccccgc
     8881 ctcccgctgg cccgccccca ccctgtccgc gcgcgacggc gcgctctggc tggaggagct
     8941 gccgctcgcc gagatcgccg cgcgcttcgg cacccctacc tatgtgtact cgcgcgccgc
     9001 gctcgaggcc gccttcgcgg cctggaagga cgcgctcgcc gggcgccggg cgctggtgtg
     9061 ctacgcggtc aaggccaact ccaacctcgg catcctgtcg gtgttcgcgc gcctgggcgc
     9121 aggcttcgac atcgtctcgg gcggcgagct cgcgcgcgtg ctggccgccg gcgggcgtgc
     9181 cgacaaggtg gtgttctcgg gcgtcggcaa gacccgcgcc gagatgcgcc aggcgctgca
     9241 ggcgggcatc cgctgcttca acgtcgaatc ggccgccgag ctcgagcacc tcgacgcggt
     9301 ggcaggcgag ctcggcgtgc gcgcgccggt gtcgctgcgg gtcaatccgg acgtcgaccc
     9361 caagacccac ccctacatct ccaccggcct caagggcaac aagttcggcg tcgccttcgc
     9421 cgacgcaccg gcgctctacc gccgcgccgc ggcgctgccc aacctgcgca tcaccggcgt
     9481 cgcctgccac atcggctcgc agctgctcga ccgcgcgccc atcgccgagg ccgcacagaa
     9541 gctgcgcggc ctggtcgaag gcctcgccgc cgacggcatc gcgctggagc acatcgacct
     9601 cggcggcggc ctgggcatcc gctatcgcga cgagacccca ccaccggtcg cggaatacct
     9661 cgccccgctg ctggaggtct ttgcggatcg cgaggaagag ctctgcttcg agcccggccg
     9721 ctccctggtc ggcaacgccg gtctgctgct gacccgcatc gaatacctca agccgggcga
     9781 aacgaagaac ttcgcgatcg tcgatgcggc catgaacgac ctcgcccgcc ccgcgctcta
     9841 cgacgcctgg cacgaggtgg tcgaggtcac gccgcgcgcc ctcgccccca ccacctacga
     9901 cgtggtcggg ccgatctgcg agagcggcga cttcctcgcc cgcgcgcgca gcctcgcggt
     9961 ggccgagggc gacctgctcg cgatcctgtc ggcaggcgcc tacggcatga ccatggcttc
    10021 aaactacaac acccgcccac gcgcggccga gatcctgatc gacggcgcca gcgcgcacgt
    10081 ggtgcgcgag cgtgagacgg tggaagagct gttcgcgcgc gagcgcacgc cggggtaaga
    10141 cggcgggagc cctgtggcga gagcagcagc cgcagatccc gcgatcggac cgacgcacct
    10201 gctcggcgta tggggcccgc ctcaggcgct cgccaccggc gggcgcgccc cggcgtcgta
    10261 ttcctcgttg agcgcgcgcc cgaaggcctt ccaggccggt ccgccgtgcg tttcgaagcc
    10321 gatctcggcg ctctggcgca tgttctgcag catcagctcg gagagcgcga agagctcgcc
    10381 ggtgagcttg tcctggcgca ccgggctgcg cagcagcttc atcatgcagg cgggcgtgca
    10441 agtcagcgca cggtgtgcca gctcgcgcat cctcgacgcg tcgccccagt cgatgccgat
    10501 cgcgatgccg cgcttgacga tttcacgctc cagttcatcc gcggtgcgcg tgtagtgtcc
    10561 gaatccgctc atctttcccg tgtatgcctg ccgcgtgact gcagatgcgc ggcggatgtg
    10621 ccgctccgaa tggaaaccgc cgcgcagctg cgccggcaaa gcgcgcattc tagcgcctcg
    10681 cccgcctgca ctcgatcagg atccctgagt cgcccatgaa gaatccttcc ctttgcgtgc
    10741 tattcatcgc cggcctgctg ctggccccga tcacccacgc cggcgtcgcc ggcctgccgc
    10801 ccggggtgcg ccttgcactc gacagcgcgc gcatcccggc agaggcggtg agcctctgga
    10861 cccgccctgt ggaccacgcc acgcccacgc tcgcaatcaa cgccaccgca ccgatgaatc
    10921 cggcatcggt gatgaagctc gtcaccgcct tcagcgcgct cgaacgcctc ggccccgccc
    10981 acacctggac gacccgcatc gcccgcgacg gcccgatccg tgccggggtg ctggaaggca
    11041 acctctacct cgtcggcggc gccgatccgg tactgggcta cgaccgcctg tggaaggtgc
    11101 tgcgccgctt gcgcgcgctc ggcatcgagc gcgtggcggg cgacatcgtg ctcgatgcct
    11161 cggtgctgcg cctgccgccg cacgaccccg acgccttcga tggccgcggc gtgcgcccct
    11221 acaacagcgg tccgtacggc ctgttgctgc aatacaacac cctgctgctc ggcctgaccc
    11281 cgggcaaggc ggccaacgcg ccggtcgccc tggccgccga accgccgctc gccgggctcg
    11341 tcatcgacaa ccgcgtcctg acctcggacg cctcttgcgg cagctggtac caggacctgc
    11401 aggccgtggt cgaacccggt cgccgcctgg tactgagcgg cagcctgccc gccgcctgcg
    11461 gcccgcgcac ctggagcgcc gcgccgctgc cgccagccga attcggcagc gcactggtcg
    11521 ccgcgttgtg gagcgaggtc ggcggacacc tgggcgggac ggtgcgcgaa ggccgcctgc
    11581 cgaccggggc ggaagaactg ttctccgatg actcggatcc actcgcggac gtggtgcgcg
    11641 acatgaacaa gtggtcgagc aatgtgatcg cgcgccagct gcttgccacg ctgggccggg
    11701 tgagcctgcg cgaggacgcg accgaggaca tggtgaccgc gggcgcacgc gcggccgccg
    11761 cgacgctggc cgccgccggc atcgacaccg acgggctggt gatcgagaac ggctcggggc
    11821 tgtcgcggat cgagcgcatc cgcgccgaca gcctgggaat gctgctgctg gccgcctggc
    11881 agcggccgtg gatgccggag ttcatcgccg ccctgccggt ggcgggcgtc gacggcaccg
    11941 cccgccgccg cctcggcgac agccccgcac gcggccaggc ccacatcaag accggcacgc
    12001 tcaatggcgt gcgcgcgatg gccggctacc tgctcgaccg caatggccaa cgccacgcgg
    12061 tggtgatgat ggtcaaccac cccgaggccg ccgccagcgc cgcggcgcag gacgccctgc
    12121 tggagtggct gtggagcggc ggggaatgag gcccacgcgc aacggcggcg ccgcattcgc
    12181 gccttccggc gctcctgcgg gtcaggaagc accgcggctc ctgtaggagc ggcgcaagcc
    12241 gcgaatgcgg cgctccggca agcgctgcgg caagcacagg cgcccgctgg ctcgcgccag
    12301 ccacgactcc tccatcgcga cgtcacgcca tcggcatcgt ctgccgctgc atcaggttcc
    12361 accacccgcg caccacgcgg tagaggatcc acagcccggc gatcaacagc agcgcgagcg
    12421 cgaagggcac gccgatgatg gtcaccacca tcagcaccga caccgcgatc cacagcaccg
    12481 cgaaccagaa ggtgcggatc tgccagcgga agtggctctc cagccaggta ccccgcaccg
    12541 cattctgatt ccagtagttc aggatcaccg cagcgatcga gggcaggctg gccacgaagc
    12601 tgaagatgat ggtggccgag cccacgaccg ccgagaacac cgcgaaggcg tgcagcgcat
    12661 acaccaggtg ggtgacggtg accatgttct cggacggctg gccggcctcg agcggggcgg
    12721 ggtagggact gttcatcgac tttcctcgaa cggaagcccg cgccggggac atccgcagcg
    12781 catgacaaag gcttcgaccg cggaccatga cgggtgttcg gctgcagtgc aacaacaaaa
    12841 cgtaagccta cagccccagc tcgccccaca tcgcatccac cttcgccttc acggccgcat
    12901 ccatcacgat cggcgtcccc cactcgcggc tggtctcgcc cggccacttg ttggtcgcgt
    12961 ccagccccat cttgctgccc agccccgcca ccggactggc gaagtcgaga tagtcgatcg
    13021 gcgtgttgtc gaccagcgtg gtgtcgcgcg tggcgtccat gcgcgtcgtg agcgcccaga
    13081 tcacctcctt ccagtcgcgg atgttcacat cctcgtccac cacgatgatg aacttggtgt
    13141 acatgaactg gcgcaggaag ctccagatgc cgaacatcac ccgcttggcg tggcccgggt
    13201 actgcttgcg gatgctgacc accgccaggc gatacgagca gccctccggg ggcaggtaga
    13261 agtcgacgat ctcggtgaac tgcttctgca gcagcggcac gaagacctcg ttcaaggcga
    13321 cgccgagcat cgccggctcg tcgggcggct tgccggtgta ggtgctgtga tagatcggat
    13381 cgcgccgcat ggtgatgcgc tcgatcgtga acaccgggaa atcggagacc tcgttgtagt
    13441 agccggtgtg gtcgccatag gggccttcgg gcgccatgtc gcccgggtga atcgcgccct
    13501 cgagcacgat ctcggccgac gccggcacct gcaggtcgct gccgagacat ttcaccagct
    13561 cggtcttggc accgcgcagg aggccggcga actggtactc cgagagcgaa tcgggcaccg
    13621 gcgtcaccgc gccgaggatg gtcgccggat cgcagcccag caccaccgcg accgggaaag
    13681 gctcgcccgg atgcgcgcgc tggtgctcga ggaagtcgat cgccccgccc cggtgcgcca
    13741 gccagcgcat gatcacccgg ttgcggccga tcacctgctg gcggtagatg cccaggttct
    13801 ggcgcttctt gtgcggcccg cgcgtcacca ccaggcccca ggtgatcagc ggcgcggcgt
    13861 cgcccggcca gcagtgctgg atcggcagct tcgccaggtc gacctcgtcg cccgaccaca
    13921 ccacctgctg gcagggcgcg gagcgcacct ccttgggcgc catgttgagc acctgcttca
    13981 gcaccggcag cttgtcccag gcgtccttca gccccttggg cggctcgggc tccttgagat
    14041 aggcgagcag cttgccgacc tcgcgcaggg gcgtgctcca gtcgccgtcg gcgacctcct
    14101 cccccatgcc catcgccacc cgctgcggcg tgccgaagag gttggcgagc acggggatcg
    14161 cctgcgccac gcccctggtc accggcctct cgaacagcag cgccggcccg ccggcgcgca
    14221 gcacgcggtc ggcgatctcg gtcatctcga ggtgggtgtc gacgggggtc ttgatgcgcc
    14281 ggagctcgcc acgggcttcg agctgggcga ggaagtcgcg gaggtcgtcg tatttcattc
    14341 gtgcgtctcg tgatgggtgc gaagcagcgg ccggcgttga cgcacccgcc gccggtaagt
    14401 tgccgccgcg agcgatcgga ctcgatcagc ggtacgcatt gatcgcgtcc cgcgtctcct
    14461 ccgccaccct ccgcgccgct tccgcgaagt cttccccctt ccccgcatac agcaccgcgc
    14521 gcgaggaatt gatcatcagc ccggtgccgt tctccgtgcg ccccgccgcc agggtcgcgg
    14581 cgatgtcgcc gccctgggcg ccgatgcccg gcaccagcag cggcagctcg ccgacgagcg
    14641 cacgcacgcg tgcgatctcg gccgggaagg tggcgccgac gacgagcgcg cagttgccgc
    14701 tggcgttcca gtcctcggcg acggtgcgcg cgacgtgttc atacagcttc atgcggccct
    14761 tcgacgtatc gacctccaga aactgcaggt ccgaaccgcc cgggttggag gtgcggcaga
    14821 gcaggatcac gcccttgtcg gggtaggcca ggtagggatc gaccgagtcg cggcccatgt
    14881 agggattgac cgtgatggcg tcggccttga agcgctcgaa ggcctcgatg gcgtactgct
    14941 cggcggtgct gccgatgtcg ccgcgcttgg cgtcgaggac gaccggggtg ccgggatggc
    15001 gggcgtggat gtgctcgacc agcgcctcga gctgatcctc ggcgcggtgc gcggcgaagt
    15061 aggcgatctg cggcttgaag cagcacacca ggtcggcggt ggcgtcgacg atctgcgtgc
    15121 agaaccggag gatggcgtcg gagcgcccct gcaggtgggc ggggaacctg gcggggtcgg
    15181 ggtcgaggcc gacacagagc aggctgtcgc gctgctgcca ggcggcgcgg agggcggtca
    15241 tgaagtgcat ggggatcggt ccggtgtgag gcagagcgcg ccgcgcgccg acagggctca
    15301 agcgcgcagc aggaggtcga tgaagaggcc ggcgaagatc gcgccgccga cccagttgtt
    15361 gtggcggaag gccttgaagc agtcggcgcg gtcgcgcgcg cggatcaggg tgtagtggta
    15421 gccggcgatg gcgcaggcga cgaccaggcc tgcgtaccag gcgaagccca gcccggcgag
    15481 cgcgccgacg acggtgagca gcacgaaggt ggcggcgtag cacagcatga ccgccagcac
    15541 gtcgtggcgg ccgaaggtga tcgccgaagt cttgatgccg atcttgaggt cgtccgggcg
    15601 atcgaccatc gcgtattcgg tgtcgtaggc gacagcccag aagatgttgg ccagcagcat
    15661 cagccagccg gtcgccggca ccgtgccggt gacggcggcg aaggccatcg ggatgccgaa
    15721 gccgaaggcg atgccgaggt aggcctgcgg gatcgcgaag aagcgcttgg tgaagggata
    15781 gctgccggcg agcagcagcg cgggcaccga gagcaggatg gtgagggtgt tgagcggcag
    15841 cacgaggacg aaggcggccg ccgacagcgc ggcggcgagc gcgagcgcct cgccggggct
    15901 gaccacgccg gtggcgagcg ggcgagtgcg ggtgcgctcg acgtggccgt cgaagtcgcg
    15961 gtcggcgtag tcgttgatga cgcagccggc ggagcgcatc aggaaggtgc cgacgatgaa
    16021 gatcaccagg atgtgcagcg gcggccagcc tgccgcggca atccacagcg cccacagcgt
    16081 gggccacagc agcagcaggg tgccgatcgg cttgtcgatc cgcatcaggc gggcatagtg
    16141 gggaagcttg tcgctcaggc tcatggcggg ggtcatttgc gcagttccag gattgcgggg
    16201 aggaaggcct cgctcaccat cagcgcgcgg ccccgcagca ggaagaccga gcgccgcgcc
    16261 cacagcgcgc gcggcagtgc cggcgcgcgg gactggcggg cgagcgccgc ggccgcgcga
    16321 tgataccgcg catcgcgccc gtccagacgc ctgcaggcga gcggcaggcg cgcgatcgcc
    16381 gggtcggaga acagcgccgc gccgagcggg cgggcgccga tgccgtggaa gaggttccac
    16441 gccccgcgca ggttgcggcg gggcagcagc gagcgcgcga acaccaccgg acggccgtct
    16501 gccaccagca gcacctcgcg cagccaggcc agctcgccga cgcgcaggcc gagcagccac
    16561 gcctcgtcgc gccgcacacg ggcgaggcgc tggcacagca cgcgcacctc gaagcgggtg
    16621 cagcgcgcgc ggatgcgcgc ggtgagcgag ccggcatcct tcagccaggg cagcagcgcg
    16681 ggcgggagca agacgcgcgg cggatgggcg agccaggtct cgtggcgcgg gccggtgtgc
    16741 agggggaagg cggtcagggg gcgcatcggg tcaggagcgg gtttcggttt cttcgtgcag
    16801 gagcaggccg cttgcggcgc aagggcacgc gaggggtgtc cggacggcag caatcgcgac
    16861 ttgcgtcgct cccacgggaa acgcggcgcg tctgggccgc agcaatcgcg acttgcggtg
    16921 ctcctgcgtc ccacaccggg cgctccgggt tttccgtggg agcgacgcaa gtcgcgatct
    16981 gccgttctcc acccgatgcg cggcgaaaag tgccacaggg caccgacagc actcgccagc
    17041 ccatcatgcg cgcagcaagg cttcgcgccc gcccagccag cgccgcatca gcgcgttggc
    17101 ggcggcgggg tcgtgggcga gcaggtgctc ggcgagctcg cgtgcgggct cgaccagccc
    17161 ggcgtcgagt tcgagatcgg cgtagcgcag cagcggcacg ccgctctggc gggcgccgac
    17221 gaactcgccc gggccgcgga tgcgcagatc ctcgcgcgcg atcgcgaagc cgtcggtgtt
    17281 ctcgtagatc accttgaggc gctcgcggcc ggtctgcgat agcggctggg cgtagatcag
    17341 gatgcatacc gactccgccg tgccgcgccc cacccggccg cgcagctggt gcagctgggc
    17401 gagcccgaag cgctcggcgt gctcgatcac catcaggctg gcgttgggca catccacccc
    17461 gacctcgatc accgtggtgg ccaccagcag atcgagcgcg ccggcggaga aggcggccat
    17521 ggtggcggat ttctcctcgt tcctgaggcg gccgtgcacc aggcccacgc gcagctcggg
    17581 gagcgcctcg accagcgtcg cgaaggtgtc ctgcgcggtc tgcagttgca gcgcctcgga
    17641 ctcctcgatc agcgggcaca cccagtaggc ctggcggccc tccagacagg ccgcgcgcac
    17701 gcgcgcgacg acctcgtcac gacgcacgtc ggagaccagg cgggtgcgga tcggcgtgcg
    17761 cccgggcggc agctcgtcga gcaccgacac gtcgagatcg gcgtagtggg tcatcgccag
    17821 cgtgcgcggg atcggcgtcg ccgacatcat cagcatgtgc ggccgccgcc cgccctcgcc
    17881 cttctcgcgc agcgccaggc gctggcgcac gccgaagcgg tgctgctcgt cgaccaccac
    17941 gagcgccagc cgcggcagcg tcaccgggtc ctcgatgagc gcatgggtgc cgaccgcgag
    18001 cagcacctcg ccgctcgcca ggcgggcgag ctcggcctcg cgcgcgcgct tcctgcggct
    18061 gcccgacagc caggcgacgc cgatgcccag gggctccagc caggccgaga gcttcttcca
    18121 gtgctgctct gcgaggatct cggtgggcgc catcatcgcc gcctgccacc cgttctcggc
    18181 cgcctgcagc atcgccagcg cggcgaccag ggtcttgccc gagccgacat cgccctgcag
    18241 caggcgctgc atcggatgcg gcgccgcgag gtcggcggcg atctccgcgc ccacccgcgc
    18301 ctgcgccccc gtgggacgga aaggcagcga ttcgagcaaa gccgcggtca gcagggcgcg
    18361 cgccggcagc gccaccgccg cttgggcacg gcgcgcgagc caggcgcggc gcagcgagag
    18421 ctgctgcacc agcagctcct cgaacttcat gcgctgccag gcgggatgct cgcgctgctc
    18481 gagcgcgaag gggtcggcgt cgggcgcggg atggtgcagc gcatgcaagg cctcggcgaa
    18541 gccgggcagc cccaggcggt gcagccaggc ggccggcagc agctcgtcga gcggctcgct
    18601 ctgcagcgcc tgtgcgacca gcttgcgcag ggtgatctgg ccgacgccgg cggtggtggg
    18661 atagaccggg gtgagcgcat cgggcagcgc ctcgccgggc gccacggcgc gcaggcgcgg
    18721 atgcaccatc tcgagcccga agaagccgcc gcgcgcctcg ccgaacaggc gcacgcgccc
    18781 gcccggcgcg agcggtttct gctgcgaggg gtagaagttg agccagcgcg ccaccagggt
    18841 gccgctgtcg tcggcgatcc gcgccaccag ctggcggcgc ggacgcagca ccacctcgct
    18901 accgagcacc tcgccctcga cctgggcggg aaagccgggg cgcaaccggg caatcggcgt
    18961 caagcgggtc tcgtcctcgt agcgcagcgg caggtggagc aggacatcgc gtggcccgcg
    19021 caggtcgagc ctggccagcc gccccgccag ctgcgccccg acccccggcc agccgcgcgg
    19081 cggctgcgtc gcgttcaatc gtcgatcctt ctcggaagga ggaaaggggc aacaggtctc
    19141 gcgcgcccgc cgttgatcgg ccggcacgcg accgggacac cgaagcggta tgccaaggag
    19201 gcagcgctcc gagcgccccg ccccttaagt ttcacacctc gtgccttgcc ccttgcaggc
    19261 ctcagccgag cacgagcacc agatccgcct cgaccagcgc gccgcgcggc agctcgcgca
    19321 cgccgacggt ggcacgtgcc ggatagggct cggcgaagca ggacttcatg atctcgttga
    19381 ccacgccgaa gttggccagg tcggtgagat agatggtgat cttgaccacg tcggcgaagg
    19441 agccgccggc cgcctcggcc accgcggcga ggttgtcgaa cacgcgacgc gcctgcgcct
    19501 cgacgccctc gaccatttcc atggtgacgg ggtcgagacc gatctggccg gagaggaaga
    19561 cctggttgcc gaccttgacc gcctgcgaat aggggccgat ggcggcgggt gccttggggg
    19621 tggagatgat ctgacgggcc atggttttgc ctctgcttgg ggaaagccgg cattgtaccc
    19681 gcgggcgcgg gcggacatgc cgcccgcagg gcacccgggc ggctcagccg ccggcgcgca
    19741 gggtgatctc gcagccgagg cggtcgatca gtttctgcaa gccgacacgc tcgccggtgt
    19801 tgatgaccag cggcgccatg aaattggcgc gcaggccggt gccggccggg ctttcgcctt
    19861 cgtccttgcg cacgatcacc gcgaccgcgg catcctcggg cgtgcgcagg cgcagcatcg
    19921 cgacttcctc gtcgctcagc gggaattcca ggtgaacgcc gaggcgctcg ggcgcggtca
    19981 ccgagaaggc gacatcgggc tcgtccacgc tctgcagcag gaacacatgc gcctcgcgtc
    20041 cttcctcgtg cagcagggtg aagcggcggc actcctcgaa gccgggcagg cccttgggaa
    20101 actcgatgat cttgtcttcg gcaatctcgg cgctgccgaa cagcggactc ttgatctgca
    20161 tgactggaac ctcgcttcaa aaaaacatca tcatccgagg cacggcggac ccgccgtgac
    20221 acgcttcacg tccgctcaac gcgggcgttc tggggggatc aacacgccag gcccggcacc
    20281 cgccgcctgc agccgcttgc gcgcacgctc ggcggcctcg cggtcgacga agggaccgag
    20341 caccacccgg ctctggatgt gcgccggctg ccccgcggct gcggcctgct ggtagatgcg
    20401 cagcgcgttg tcgggggcgg cgaagacgcc gagctggacg agatagccgg agccggcggg
    20461 cgccacggcg gcacggggaa gcaggacacc atcgagcttt gcggtgggaa ccggaagcgg
    20521 agtgcatgcc ggcgcaggtg gcggagtcgg gcaggagggt gggggcgcgg atgggggcgc
    20581 gcatgcagcc ccctgcccgg ccccggccgt gagcggcacg gcggcctcgt acgctggcgc
    20641 accggggccg ggctttccgg ccgccggcag cgggttcgcg ggcat
//
LOCUS       DAOQNN010000014        20228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_956_length_20228_cov_7.510831, whole genome shotgun sequence.
ACCESSION   DAOQNN010000014 DAOQNN010000000
VERSION     DAOQNN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 20228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20228
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_956_length_20228_cov_7.510831"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PKV97_02015"
     CDS             <1..1085
                     /locus_tag="PKV97_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04679.1"
                     /translation="RALLVERAGILALPDLERLLIPHALPDLPRVRLANPEPAFLPCG
                     TEVDDTHRERRRTGERPPAETPRVPADIVPPILRTLARLRPDVQCLLTLPLDHQPQDE
                     LPAPARDFLAHVARIVGDTGAEPAGEADDAPHAELAGKLRHLQLLWPYLRSADRPLAP
                     ACGLIAGMQAAVSLRYGAWRSIAGRPLPGKALPWPPPTQQAATALRATPGATVVLTRA
                     GRLQVDDEALCVPCLPGADLQRMTKAQRGLEHWRSAEVMRFMGWLRRELQALGERLLF
                     DTDPGDPRPELALRGFFGRLHEAGALRGAQPEHAYRIRELPAVDSAIAFEIEIAPAYP
                     IDHLRLTFLHDRHAAVTETRIEAIDG"
     gene            1078..1542
                     /locus_tag="PKV97_02020"
     CDS             1078..1542
                     /locus_tag="PKV97_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNB04680.1"
                     /translation="MADSAGEFIPFRFRVALYDGEGGRLLCRGAFSEVSGLEATMAPK
                     TLREGGRNWGEVQLAGPTSFAPVILKRGITEVADLWNWFDVSTRQSNHALRLSGTIDV
                     YHPQDPDKPLLRWRITNALPTRFKGPDLSSTASQVAIEELQLVHEGLELERG"
     gene            1557..2276
                     /locus_tag="PKV97_02025"
     CDS             1557..2276
                     /locus_tag="PKV97_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04681.1"
                     /translation="MPAPQIARAKLIPMNGDQVETDESKHIDVQFNPATLRVTLSNTL
                     KADNRAGSGGSGAAAQYIDKSESTLAVELVFDTSVAAKLGSDARANRETVAADSDVRT
                     LTKRIAETFMKPQDAGSNRPRAPGRCRFQWGSFQFTGMLSSYSETLDFFAPEGIPLRA
                     TLALTFKEDRYQFALDPTVRADARPMPGFAAGGEGRDAASAAQSAGRDPRDWRVIAEL
                     NRLDNPRFTVPGALLIPTGGT"
     gene            2276..2440
                     /locus_tag="PKV97_02030"
     CDS             2276..2440
                     /locus_tag="PKV97_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04682.1"
                     /translation="MPLIIDELVAEVQAEPNPAQASATEARPPAGPPGDTLLDLLALA
                     GEREARLAID"
     gene            2469..3476
                     /locus_tag="PKV97_02035"
     CDS             2469..3476
                     /locus_tag="PKV97_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HNB04683.1"
                     /translation="MRVDGDDRPALGTAVQALSVHLPASGMASAEARVVNWAAGEGAA
                     PDFAFQDLRLGSSIEILLGEHASGAVFVGEITALEERYGDGAPQLVILAEDALHRLAR
                     RRASRVFENRGLAAVVRAIADDAGLQADVGIEVGSADWLQHNESDLAFLLRVLGPHDI
                     PLRMQDGRLHARAEEADPDPVRIDAGANARRIRIIADLNQQPREVLAHGYNLATDSST
                     QGSSAALAPAPPGRSAATTLAELGWEGRSPRPHPFPRTQAEAESLARGAFRRDARRFL
                     HGEIVCTGTPELGVGRELELTGVSPRLAGRYRIAECWHHFDNTQGLSTRLRVERPDWT
                     P"
     gene            3490..4128
                     /locus_tag="PKV97_02040"
     CDS             3490..4128
                     /locus_tag="PKV97_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HNB04684.1"
                     /translation="MSRHSPGPLQELHLGVVLDNDDPDDRGRVKLHLAATGLETWAAV
                     MVPSAGGGYGVSLLPRVGEQVVVAFVAPDLPIVLGAVWSGGASQPEDARPVDERYLVQ
                     SPAGLKVLLDDRQPSVTIETPAGNRLVITDQGGGKVTIEQGGERVDIAPGTVKLTGSG
                     IVSIEAAQVTISAGLVKVDAGLSKFSGVVQCDTLISNAVVSTSYSPGAGNLW"
     gene            4122..5774
                     /locus_tag="PKV97_02045"
     CDS             4122..5774
                     /locus_tag="PKV97_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04685.1"
                     /translation="MVSIDSALRAERPMRPARGAPSAAPHQPQVIAPFFAGEGEGLTL
                     HRYFDEDFVARFLNDAQAGRLTGTAAQPWFRADRFGRFGAEPTLRLPLHRCFYLVCAE
                     VRCMAAGGPAFDPRKILGAGLVVRRVAADGSAQRWMVADGQPLGWRDGRIPDHDPDDV
                     HRLRARGLLPVRFPEPPYSGEEVAPLHTLLATRRDARGVERKHTLLWGYLPLSGSARV
                     QAEAPLPQTDGGDAPDFGLEHAWPLGTRDARPWVDGDGLVVTEGVASVGFVELLQTLV
                     AQFRIDDATDPDNAGLRTLLGQMRFHHFEVRLVEGEGFPQRVPVFGDTALDWLDRSAA
                     ALVEFFARIDRGEFVAGRTALPDGTGGSRKDRLLLTEQQAENLRSLVGLRLARAQVRF
                     DDGLPMPRYGQGDDDRFTALPFIRWHDECGCERVAWGPASHVFRVASPFDPEAQRPTT
                     VVLPALDDLKRGLPRGVAMLAPKSLADVLRKISPDADMKGDGPGNRSGACWSFSFSLP
                     AITLCATMLLMVVINLLNLFLGWLPRVFLALPRLCLKALRGK"
     gene            5794..6183
                     /locus_tag="PKV97_02050"
     CDS             5794..6183
                     /locus_tag="PKV97_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HNB04686.1"
                     /translation="MPTPESLLTSFPLLASPVAGGRQHWQTGNASVREVMLNILLTRP
                     GERLMRPEFGAGIRSFIHHPNNETTRALIADAALRALRRWEPRVSIEAVRAVADPERL
                     SQVNLEVRYRLLADERRETLQLALDLG"
     gene            6301..8493
                     /locus_tag="PKV97_02055"
     CDS             6301..8493
                     /locus_tag="PKV97_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HNB04687.1"
                     /translation="MSPPKPFPVPDLDDRRFDELVAELQARLARHVPELAPLAPGDPV
                     FALVDLFAWLTETILYRANRIPDRQRLAFLNLLQIPLRPARPAGGIVSIDSTAHALPL
                     PIPPEALLKAGTTSFSTIGEVQPTPLTLRIVVKRRLDEAALAAEGISLDQLRAQYGVE
                     PAAFRPLTLHPGRDRPSAAGTCDGALHLGICLARPAPATQAERVRRALGGVLLNVGLA
                     PDTELEGGLATGLRARRLTWELAWWPDPEDAPQRVQYLPLEVVADSSAGGRRTGVARL
                     RLPRDAEMLRATSALDPQFAGLGDTPPEAPADLRPDQLLFWLRLSCAEEPDLTLGYLG
                     LNAVEVIGQGIARDVLLGAGTGRPDQRLELPDPDVDERSVEIEVEARRAFRAWTRVAH
                     FAGSGPDDTVFVVDSADRSVQFGDGVRGMRPPAGARIRAAFYRHGGGSGGNLPPGSIR
                     ELHGASEKLAVRHEWPTRGGVDAESLDEAERRIPAFLSHRDRAVTAEDFAVLARDNPI
                     NPVARAEAVAGFFPGASLAAVRRNVPGVVSVFVMPPAPQAMAAAPRPNAGLLADVHAY
                     LSARCMLGTELYVLSPRFVPIALALSLEVVDPTSEQQVHAEVERALLRYLWALPPGGP
                     RGAGWPRGKAVEINELRTQAGRVEGVEAVNALRLFYRTTEQDPPSWRELTGAQALTLL
                     DFELPELMAVSIRPGEDRPAPPRGFGPEAGAAADGPRPVPVPVIPDLC"
     gene            8508..10937
                     /locus_tag="PKV97_02060"
     CDS             8508..10937
                     /locus_tag="PKV97_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04688.1"
                     /translation="MNSNGTRFLLLDGAADFHNTSRQCSWDHEQRAFTLTRQDAPRLP
                     RLAAARARERLLAATPWMLDEHGQLGRLSDDGLRLECAPSWPPRTWQPVRATLDETHA
                     DASALEVLVLDPVDAPAGRFTDLAFGGSGLVVLPWSDGGVQHGLTAVHLRRRWQARCA
                     LPFAPRRAWVEAASGTDRVWLLGETQLGLAVGAPLPQPYRGRPERFEPLQTNPDPLRL
                     AWTLALPPHGGLLGLCTDEDHLFVLGETPDSTADAPRMQIFMRALAAAPGEGFDIRRL
                     PDGLPLATDLAAAGEGRLLLLPPIDEGAEPGTRRDCPLISLREDAPTAELVPERWPRR
                     PAAALPAADRFVRHRDGRPRALSADGPVRLYRLAQARFAPNGTVTLGTPLDSAMPDTL
                     WDRIFIDACIPPGCRIDFAVQAGDDRENLPAEWIAQPQPVLTAVSSELPFASGRAPRS
                     GDHADRSGLFELLIQRASGAVREVRGRYLRLRITMHGDGRHSPAIFALRVQYPRFSWQ
                     THYLPEHFQQQERPLASAEANQAEANGADFRERLLASFEGLLTPIEDRIAAAEILLDP
                     AVAPVAHLPGLAAMLGTTLPPHWPEARRRRWLGAQGMLQQSHGSYRGLLLALDILTDG
                     AVARGAVIPVEHFRLRRTMATILGVDMDDRDHPLTLGTGLSGNSLVGDSLILSDDLAR
                     EFLALFAPEVAEAKGEAAVVERFFEEAARRMTVILHGPARRLAAVVRDALPALVPATV
                     QWAIRSSEHPFVPGLSPLLGIDTWLEASPPARPVVLDRTRLGRGDLLHNPVALDPEHA
                     VPIDATVLDAP"
     gene            10960..11898
                     /locus_tag="PKV97_02065"
     CDS             10960..11898
                     /locus_tag="PKV97_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04689.1"
                     /translation="MSEPDDKRDTSLASDGRFEEMLKRVNYAPGMLLGIEATLAEQDY
                     HRRRATRHGYWLHGAGTVAGLRVSLQSKDPGNDTENVRVRLVVSPGIGVDGLGRELSV
                     AEPYCVDLGAWLTTQHEEPERWNALIRDGYAADDNLLWLKVTMRYQDCASGLQPVLAT
                     ELNAGTDPVQPSRVADCVLFELVAERPDDAPAEEHLFAAHARIRPYDEIEDKLGERER
                     AQVEAATGGARAQLELGARLLHSLGDDNRALVARQAFLTDPAALARTLLARIAIRLAP
                     PAPDPDLIVNPRRIEIDNLVRPFLFNAVALARLIRA"
     gene            11908..14097
                     /locus_tag="PKV97_02070"
     CDS             11908..14097
                     /locus_tag="PKV97_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HNB04690.1"
                     /translation="MANIHFTPLRIGTSNAAGERISSVDPLLSRTNYFDGQLLKAADL
                     ARDQIYLDERLLELGQVFGAGIVRGLEPSLHSGHLLEVSPGIAIAPSGRVLQLSNTAL
                     RIDLLDSGLIATLNDGRFRGPARGLHALALTHTQVVDGVAEAFPKDLSTARVPHVAAW
                     AEGVELRLMPLPLPLPRQDEIAVRASLARELLSGGERLALPSDDAVALGLIAVERGRI
                     LWLDRGLLRRPQRPPNTPDAIQQDLATHYQELMQAVLAARRSAGLRGAFAAGQYFRVL
                     PPWGPLPQEAIDPVGGRQQFFPQDYEVAIAPVRRSELAAVLADSARLAPIDLERDADC
                     DVMVLVPMSDSAFALRARQLEAPAEFRPRALGRLANLDRLALRILPRPPIHRVDTDAD
                     VWRAIWAEADPAELMYVRRPPRTAETGVSALVLALGASLPPPGSALPPDVAALEERLD
                     AALEAVDAAAQARTTLENEAARLRLRIAKLEKALAATGTGVPDDLLAEVERLGLALET
                     ARKEIAALEAEDRNAAILANALEAASDRIDALKLALDAANAEIERLKTASAPVDETLE
                     ARIEELVAALGSERTRAATLEQERTALSTRLAVAEATIGRLEREAAQAGDLATQLPLA
                     LLAQARGADDAGIKAATGADKLIGADARARIAAVQIVGMADRRLDPALWPTLPALARL
                     DPAVFVKLRDHFLAAAGQLDLAKFFLEEGPGLGLSGALLELWKRVLG"
     gene            14154..15893
                     /locus_tag="PKV97_02075"
     CDS             14154..15893
                     /locus_tag="PKV97_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04691.1"
                     /translation="MATLSFIPPGLLAALASRPAAALLPRTPPSIRLHEPQRRPDDPR
                     RINLFPGRALGEREFERLQAYVDDRIAPLLASLAPGIVEGLACALFGTGTDTRVRVGP
                     GLAVGAAGQLVRLFYPLDQAWSELVAQAERDQDAPLRDGLFLLTIRSAVEEIDDATAA
                     EPCTRSEPDALRERRIETVSLLGLQRIAGSPRMMAMPQARAANRIGVRLLRESPFRAD
                     SGGVPLGLAKIVGKLPVWFDACAGRLLAEPDAAAHSLLAHAVATLEAWQRAHPAPLPA
                     VATQTLDELLGLDFLPAAGPLPAALLRDPAARSPSLAFLPGDLRVELAPVPAGTVEEG
                     VVGELGRGPVDLIHGLGEHLRLLLAIPDLDYRRDLFDLPQRDTRLEDELFRRGERAAH
                     AYQAWKARWYALFHGLSGAQLEALRAPVLLTKAPPEPGQYRTKLVTDRAKTLRPAPQG
                     TTSHPLADLATEGTAALPEPYASHLADPHPAPDDYQPVPDIQPAGNGLLHRREALRAD
                     IRHLEAALDKSFRLLEEVGDFLGLQRQQLDALTVSFSTLAGGVPGDGLGSQLVRWTAK
                     ANFVPKPPPENPS"
     gene            15890..17782
                     /locus_tag="PKV97_02080"
     CDS             15890..17782
                     /locus_tag="PKV97_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04692.1"
                     /translation="MSDILKRFTTDASAFTELKFVDAKGTAAAVDLADATRGEATISA
                     GMLAALADLQPKAAPDEPLTEPVFSKATSTGRGMLAHVERVHDSFLNVRRDLLELRAR
                     ANARQNQAKSLIDRVCDFLDDYLKPEELRRLGVIDDEAQARPFRLLVAPDLHNAEGVL
                     LRPRLDLALPRPGEDDIEGVKHNDESALFHAGKVVADDIRQVEALRGALTTLRRRHQE
                     ALEDLRTRLVALEAELPGELRRLDVLERERTETLDDYAVAQRLLAEHWREVEAAHAER
                     RRIIEAHQGLFYVKVRETPLGRSLPDPLELRPSSPDELVPGCAGRDTALPAALAPFIE
                     AVYDIPAADWAHLRPLGHLLPGRTILAGLVEMRRQKLALRLNRPPDAGLALLSGLVQQ
                     NKALVRDIAARPFSAGALGELQRQAGAILALDDLLAIPSPQLRDPARALHQRLDTAAG
                     CLLERLRTISPSIRLNWASAAEADRLAVEAPERWPGLAEAEERDFNGVRTLVELVAWW
                     FRQLDADASAAAHGAMRNLVRACLLLAASDDPQQLVQGRLQSIPGRFRLGEALRLKLD
                     REAAPGTLLQLFDDDQRVIATLRVDDHDDQGTVASVAAILDPELERNPGAVLATGLHI
                     SGVDRG"
     gene            17788..19476
                     /locus_tag="PKV97_02085"
     CDS             17788..19476
                     /locus_tag="PKV97_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04693.1"
                     /translation="MIALADLHIGRLTLRGSATAIAHARATLPPALARARWPDEEAIL
                     VVRRIAVGGTGTELPARAAALAGQLAGRAVDPWTPAADGAEVVRFRDARDYRACLVRD
                     LLAGTAARRWLWRHRAALLAHPTATALAELLGEDALALPALLEHPPLRACLPTLWCRL
                     DAAAARSLLRAIGAATGWHGAISDALASRAAEAAGSGADLMTEAQTHHERISGDEIRP
                     GPEPGPSPPPTGPAATRAGSPRRPAPPGAAAHGTLPASDPLVVLEALLTLWRCTPAAL
                     AGPQGGEVLRGAAAALAELGGGQDAESATARASGPGRARAAGGDLPDARRRHDAGAGS
                     IPAGSPASRGFAPPASIRVGAPPRAIAAINARPAAPLRAGAPSEVTDPFAATVTATQA
                     EVDFHTGRGGLFFLLNVLNHPGLRAWRGTLHEPHAGWRELVRLALRLELAPDAPLAGF
                     LAEACALEGEQDPVAKLAGLAKLPARADPVAVDRAVLRHVGDAALRALRAERPARVRA
                     SPSHVDVHLRLAEVDLELRRGGLDLDPGWLPWLGRVVRFHYDRSDAADRGGSSP"
     gene            19473..>20228
                     /locus_tag="PKV97_02090"
     CDS             19473..>20228
                     /locus_tag="PKV97_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB04694.1"
                     /translation="MNAPASLRPESAPASEGARLSRAFVHAGIAEFARLRVGAPSAVF
                     EALAASQGGEDLATLLGTPPQDWRAACIALAQAAPELHGPLGRLLADLKLQLHEWFAM
                     ALCGENESSYLLNLALAELQSPGALRPGLHLLSAASAALFGAPLAPLALPNHRLVRAG
                     VLEIVGEGPMPTRSLAVAPELWALLCGDPTAWRDTAPLADGDTMLPRGFREQIPTLAT
                     MLRSGATRHVMFRGARAAGLAAAARLAAAAGRPP"
BASE COUNT         2787 a         8061 c         6743 g         2637 t
ORIGIN      
        1 aacgcgcgct gctggtagag cgcgccggca tcctcgcgct ccccgacctc gagcggctgc
       61 tgatcccgca cgccctgccg gacctgccgc gcgtgcgcct ggccaacccc gagcccgcgt
      121 tcctgccctg cggcaccgaa gtcgacgaca ctcatcgcga gcgccgccgc acgggcgagc
      181 gtccgcccgc tgaaacgccc cgcgtgcccg ccgacatcgt cccgcccatc ctgcgcacgc
      241 tggcgcggct gcgtccggac gtgcagtgcc tgctgaccct gccgctggac caccagccgc
      301 aggacgagct gcccgcgccc gcgcgcgact tcctcgccca cgtcgcccgc atcgtcggcg
      361 acaccggcgc ggaacccgcg ggcgaagcgg acgacgctcc gcacgccgag ctggccggca
      421 agctgcgcca tctgcagctg ctgtggccct acctgcgcag cgcggaccgc ccgctcgcac
      481 cggcctgcgg gctgatcgcc ggcatgcagg cggcggtctc gctccggtac ggcgcctggc
      541 gctcgatcgc cgggcgcccg ctgcccggca aggcgctgcc gtggccgcca ccgacgcagc
      601 aagcggccac cgccctgcgc gcaacgcccg gcgcgaccgt ggtgctgacg cgcgcgggcc
      661 ggctgcaggt ggacgacgag gccttgtgcg tgccctgcct gcccggcgcc gacctgcagc
      721 gcatgacgaa ggcgcaacgc gggctggagc actggcgttc ggccgaggtg atgcgcttca
      781 tgggctggct gcggcgcgag ctgcaggccc tgggcgagcg gctgctgttc gacaccgatc
      841 cgggcgaccc gcggcccgag ctcgccctgc gcggcttttt cgggcgcctg cacgaggccg
      901 gcgccttgcg cggcgcgcag cccgagcacg cctaccgcat ccgcgaattg ccggccgtcg
      961 attccgcgat cgcgttcgag atcgagatcg cgccggccta cccgatcgac cacctgcgcc
     1021 tgaccttcct gcacgaccgc cacgcggccg taacggaaac ccgcatcgag gccatcgatg
     1081 gctgactccg ccggcgaatt catccccttc cgcttccgcg tcgcgctcta cgacggcgaa
     1141 ggcggccggc tgctgtgccg cggcgccttc agcgaggtca gcggcctgga ggccaccatg
     1201 gcgcccaaga ccctgcggga aggcgggcgc aactggggcg aggtgcagct cgccggcccc
     1261 acgagcttcg cgcccgtcat cctcaagcgc ggcatcaccg aggtggcgga cctgtggaac
     1321 tggttcgacg tgagcacgcg ccagtccaac catgccttgc gcctgtccgg cacgatcgac
     1381 gtctatcacc cgcaggaccc cgacaagccg ctgctgcgct ggcggatcac gaacgccctg
     1441 ccgacccgct tcaagggccc cgacctgtcc tccaccgcga gccaggtcgc gatcgaggag
     1501 cttcagctcg tacacgaagg gctggagctc gagcgcggct gaatggagac gacgacatgc
     1561 ctgccccgca gatcgcccgt gccaagctga tcccgatgaa cggcgaccag gtcgagaccg
     1621 acgaatcgaa gcacatcgac gtccagttca accctgcgac gctgcgcgtg accctgtcga
     1681 acacgctgaa ggccgacaac cgggccggca gcggcggcag cggcgcggcg gcgcaataca
     1741 tcgacaagag cgaatcgacc ctcgcggtcg agctcgtgtt cgacaccagc gtggccgcga
     1801 agctcggctc cgacgcccgg gcgaatcgcg agacggtcgc cgccgacagc gacgtgagga
     1861 cgctcaccaa acgtatcgcc gaaaccttca tgaagccgca ggacgccggg tcgaaccgcc
     1921 cgcgggcccc cgggcgctgc cgtttccagt ggggcagctt ccagttcacc ggaatgctgt
     1981 cgagctactc ggagaccctc gacttcttcg cgcccgaggg catcccgttg cgcgccaccc
     2041 tggcgctcac cttcaaggag gaccgctacc agttcgcgct cgaccccacg gtgcgtgcag
     2101 acgcacgccc gatgcccggc ttcgctgccg gcggcgaagg ccgtgatgcc gcgagtgccg
     2161 cgcagtctgc cggccgcgat ccgcgcgact ggcgcgtcat cgccgaactc aaccgcctcg
     2221 acaacccgcg cttcaccgtt ccgggcgcct tgctgattcc gactggaggc acctgatgcc
     2281 gctgatcatc gacgaactcg tcgccgaggt gcaggccgag ccgaaccccg cgcaagcttc
     2341 ggccaccgag gcgcgcccac cggccggtcc gcccggcgac accctgctcg acctgcttgc
     2401 cctggccggc gaacgggagg ctcgccttgc catcgactag cgccgcgctc ctcagcgtcc
     2461 gtccgcgcat gcgcgtcgat ggagacgacc gccccgcgct cggcaccgcg gtgcaggcgc
     2521 tctcggtgca tctgcccgcg agcggcatgg cgagcgccga ggcgcgcgtg gtcaattggg
     2581 ccgcggggga aggcgcggca ccggacttcg cgttccagga tctgcgcctg ggcagcagca
     2641 tcgagatcct cctcggggag cacgccagcg gcgccgtctt cgttggcgag atcaccgcgc
     2701 tggaggagcg ttacggcgac ggcgcgccgc aactcgtgat cctcgccgag gatgcgctgc
     2761 accggctggc gcgccggcgc gccagccggg tgttcgagaa ccgtggcctg gccgcggtcg
     2821 tccgtgcgat cgccgacgac gccggactgc aggccgacgt aggcatcgag gtcggcagtg
     2881 cggactggct gcagcacaac gaaagcgacc tcgccttcct gctgcgcgtg ctgggcccgc
     2941 acgacatccc gctgcgcatg caggacgggc gcctgcacgc gcgcgccgag gaggcggacc
     3001 cggacccggt ccgcatcgac gccggagcga acgcgcgccg catccgcatc atcgccgacc
     3061 tcaaccagca gccacgggaa gtgctcgcac acggctacaa cctcgccacc gactccagca
     3121 cccagggcag cagcgccgca ctcgcgcccg cgccccccgg acgcagcgcc gcgacgacgc
     3181 tggccgagct cggctgggag gggcgctcgc cacgccccca ccccttcccg cgcacccagg
     3241 ccgaggccga atcgctcgcg cgtggcgcgt tccgccgcga tgcacgacgc ttcctccacg
     3301 gcgagatcgt ctgcaccggc acgcccgagc tgggagtcgg gcgcgagctc gaactcaccg
     3361 gggtatcgcc gcggctcgcc ggacgttacc gcatcgccga gtgctggcat cacttcgaca
     3421 acacccaggg cctgtcgaca cgactgcgcg tcgaacggcc cgactggacc ccctgaggaa
     3481 gcgcccgcca tgtcgcgcca tagccccggt ccgctgcagg aactgcacct cggcgtcgtc
     3541 ctcgacaacg acgacccgga cgaccgcggt cgcgtgaagc tgcacctggc ggcgaccggc
     3601 ctggagacct gggccgccgt gatggtgccg agcgcgggcg gcggctacgg cgtcagcctc
     3661 ctgccacgcg tgggcgaaca ggtcgtggtc gccttcgtcg ctcccgacct gccgatcgtg
     3721 ctcggcgcgg tgtggagcgg tggcgccagc cagcccgagg acgcgcgccc ggtcgacgaa
     3781 cgctacctcg tgcagagccc tgccggcctg aaggttctgc tcgacgaccg gcagccgagc
     3841 gtgacgatcg agaccccggc cggaaaccgc ctcgtgatca ccgaccaggg cggcggcaag
     3901 gtcacgatcg aacagggcgg cgagcgggtg gacatcgcgc cgggcacggt caagctcacc
     3961 ggctcgggga tcgtcagcat cgaggccgcc caggtcacga tctccgccgg cctggtcaag
     4021 gtcgacgccg gcctgagcaa gttctccggc gtcgtccagt gcgacacgct gatcagcaac
     4081 gcggtggtgt cgacgagcta ttcgccggga gcggggaacc tatggtgagc atcgattccg
     4141 ccctgcgcgc cgagcgcccg atgcgtcccg cccgcggcgc cccctccgcg gctccccacc
     4201 agccgcaggt catcgccccc ttcttcgccg gcgaaggcga ggggctgacc ctgcatcgct
     4261 acttcgacga ggacttcgtc gcgcgtttcc tcaacgacgc ccaggctggc cggctcaccg
     4321 ggactgccgc gcagccatgg ttccgcgccg accgtttcgg ccgcttcggc gccgagccga
     4381 ccctgcgcct gcccctgcac cgctgcttct acctcgtgtg cgccgaggtg cgctgcatgg
     4441 ccgcaggcgg ccccgccttc gatccgcgca agatcctcgg cgccggcctc gtggtgcgcc
     4501 gggtagcggc cgacggctcg gcgcagcgct ggatggtcgc cgacggccag ccattgggct
     4561 ggcgcgacgg caggatcccc gatcacgacc ccgacgacgt gcaccgcctc cgcgcgcgcg
     4621 ggctgctgcc ggtgcgcttc cccgaaccgc cctattccgg ggaggaggtc gcccccctgc
     4681 acacgctgct ggcgacccgc cgcgacgcac gcggagtcga gcgcaagcac accctgctgt
     4741 ggggctacct gccgctctcg ggcagcgccc gcgtgcaggc tgaggcgccg ctgccgcaga
     4801 ccgacggcgg cgacgccccc gacttcgggc tggagcatgc atggccgctc ggcacccgcg
     4861 acgccaggcc ctgggtggac ggcgacggcc tcgtcgtcac cgagggtgtc gccagcgtcg
     4921 gcttcgtcga gctgctgcag accctggtcg cgcagttccg catcgacgac gccaccgacc
     4981 ccgacaacgc cggcctgcgc accctgctcg gccagatgcg cttccaccac ttcgaggtcc
     5041 gcctcgtcga gggcgaaggc ttcccgcaac gggtgccggt gttcggcgac acggcactcg
     5101 actggctgga ccgcagcgcg gcggccctgg tcgagttctt cgcccgcatc gaccgcggcg
     5161 agttcgtcgc cggccgcacc gcgctgcccg acggcacggg cggaagccgc aaggaccgcc
     5221 tgctgctcac cgaacagcag gccgagaacc tgcgcagcct ggtcgggctg cgcctggcac
     5281 gcgcccaggt gcgcttcgac gacggcctgc ccatgccgcg ctacggccag ggcgacgacg
     5341 accgcttcac cgccctgccc ttcatccgct ggcacgatga gtgcggttgc gagcgcgtcg
     5401 cctggggccc ggccagccac gtcttccgcg tcgcctcgcc cttcgacccc gaggcgcagc
     5461 gtccgaccac ggtggtcctg cccgcactcg acgacctcaa gcgcgggctt ccgcgcggcg
     5521 tggcgatgct ggcgcccaag tcgctcgccg acgtgctgcg caagatcagt ccggacgccg
     5581 acatgaaggg cgacggcccg ggcaaccgca gcggcgcgtg ctggagcttc agcttcagcc
     5641 tgccggcgat cacgctgtgc gcgaccatgc tgctgatggt cgtgatcaac ctgctgaacc
     5701 tcttcctcgg ctggctgccg cgggttttcc tcgcgctgcc gcgcctgtgc ctgaaggcgc
     5761 tccggggcaa gtgagaccaa cgaggagacc gcgatgccga ccccggagtc cctgctcacg
     5821 agcttccccc tcctcgccag cccggtcgct ggtggccgcc agcactggca gaccggcaac
     5881 gcgagcgtgc gcgaagtgat gctcaacatc ctgctgaccc gccccggcga gcgcctgatg
     5941 cggcccgagt tcggcgccgg catccgcagc ttcatccacc accccaacaa cgagaccacg
     6001 cgcgcgctga tcgccgacgc cgcgctgcgc gcgctgcggc gctgggaacc gcgcgtgagc
     6061 atcgaggcgg tgcgcgccgt ggccgacccc gagcgcctgt cgcaggtgaa cctcgaggtg
     6121 cgctaccggc tgcttgccga cgagcggcgc gagacgctgc aactggcgct ggacctggga
     6181 tgactgccat gcacgccgca cgccggtcga tcgcccccac cccgtctcga cgccgccagg
     6241 cgaagggtgc tgccttcgcc cacgcccgcc gcgcacccga atcctgaggg aaccgaacaa
     6301 atgtcgcccc ccaagccgtt ccccgtcccc gaccttgacg accgccgctt cgacgaactc
     6361 gtcgccgaac tgcaggcacg cctcgcgcgc cacgtgccgg aactcgcccc gctcgcgccc
     6421 ggcgacccgg tgttcgcgct ggtggatctg ttcgcctggc tcaccgaaac catcctttat
     6481 cgcgccaacc gcattccgga ccgccagcgc ctggccttcc tcaacctgct gcagattccc
     6541 ctgcgcccgg cacgcccggc gggcggcatc gtcagcatcg actcgaccgc gcacgcgctg
     6601 ccgctgccga ttccacccga agcgctgctc aaggcgggga cgacgagttt ctcgaccatt
     6661 ggcgaggtgc agcccacgcc actcaccctg cgcatcgtcg tcaagcgcag gctcgacgag
     6721 gccgcgcttg ccgccgaggg catcagcctc gaccagttgc gcgcacagta cggggtagag
     6781 ccggccgcct tccggccgct cacgctgcac cccgggcgcg accggccgag cgccgccggg
     6841 acctgcgacg gcgcactgca tctgggcatc tgcctcgcca gaccagcccc ggcgacgcaa
     6901 gccgaacgcg tgcgccgggc actcggtggc gtcctcctga acgtcgggct ggcgccggac
     6961 acggagctgg agggcgggct cgccaccggc ctgcgtgcgc gcaggctgac gtgggagctg
     7021 gcatggtggc ccgaccccga agatgcaccg caaagggtgc agtacctccc gctcgaagtc
     7081 gtcgccgaca gttcggcggg cggacgcagg accggcgtcg cacgtctccg cctgccgcgc
     7141 gacgcggaga tgctgcgcgc gacgagcgcc ctcgatcccc agttcgccgg actcggcgac
     7201 acgccgcccg aggcacccgc cgacctgcgc cccgatcagc ttctgttctg gctgcgcctg
     7261 tcctgcgcgg aggagcccga tctcacgctc ggctaccttg gcctcaacgc ggtcgaggtg
     7321 atcggccagg gcatcgcgcg cgacgtcctg ctcggcgccg gcaccggccg tcccgaccag
     7381 cgcctcgaac tgcccgaccc ggacgtggat gagcgcagcg tcgagatcga ggtggaggcc
     7441 cgacgcgcct ttcgcgcctg gacccgggtc gctcatttcg ccggcagcgg tcccgacgac
     7501 accgtcttcg tcgtcgactc cgccgaccgc agcgtgcagt tcggcgacgg cgtccgcggc
     7561 atgcgtccgc ccgccggggc ccgcatccgc gccgccttct accgccacgg cggcggcagc
     7621 ggcggaaacc ttccacccgg cagcatccgg gaactccatg gcgcgagcga aaagctcgcc
     7681 gtacgccacg agtggccgac ccgcggcggc gtcgacgccg agtcgctgga cgaagccgag
     7741 cgccgcatcc cggccttcct gtcccaccgc gaccgggcag tcacggcgga ggacttcgcc
     7801 gtgctcgcgc gcgacaaccc gatcaacccg gtcgcgcgcg ccgaggccgt ggcaggattc
     7861 ttccccggcg caagcctcgc cgcggtgcgg cgcaacgtcc ccggcgtcgt cagcgtgttc
     7921 gtgatgccgc cggcaccgca ggcgatggcg gccgcaccgc gtccgaacgc cgggctgctc
     7981 gcggacgtgc acgcgtacct ctcggcgcgc tgcatgctcg gcaccgagct ctacgtgctg
     8041 tcgccacgct tcgtaccgat cgcgctcgca ctctcgctcg aggtggtcga tccgacgagc
     8101 gagcagcagg tccatgcgga ggtcgagcgc gccctgctgc gctacctgtg ggccttgccg
     8161 ccgggtgggc cgcgcggtgc gggctggcca cgcggcaagg ccgtcgagat caacgagctg
     8221 cgcacccagg ccgggcgcgt cgagggcgtg gaggcggtca atgcgctgcg cctcttctac
     8281 cggacgacgg agcaggaccc gccgagctgg cgcgagctca ccggtgcgca ggcacttacg
     8341 ctgctcgact tcgaactccc cgagctgatg gcggtctcca tccgccccgg cgaggaccgc
     8401 cccgccccgc cgcgcggctt cggacccgag gcgggcgcgg cagcggacgg cccgcgcccg
     8461 gtgccggtac cggtgattcc ggatctgtgc tgatcgaggc cacgtccatg aacagcaacg
     8521 gcacccgctt cctgctcctc gacggcgcgg ccgacttcca caacacaagc cggcaatgca
     8581 gctgggacca cgagcagcgc gccttcacgc tgacccgcca ggacgccccg cgcctgccac
     8641 gcctggcggc cgcgcgcgca cgcgagcgcc tgctcgcggc gacgccctgg atgctcgacg
     8701 agcacggcca gctcggtcgc ctgtcggacg acggcctgcg cctggagtgc gcgccgtcct
     8761 ggccgccacg cacctggcag ccggtgcgcg ccacgctcga cgaaacgcac gccgatgcct
     8821 ccgccctcga ggtgctggtg ctcgatccgg tcgatgcgcc agcgggccgc ttcaccgacc
     8881 tcgccttcgg cggcagcggc ctggtcgtgc tgccgtggag cgacggtggc gtacagcatg
     8941 gtctcaccgc ggtgcatctg cgccggcgct ggcaggcccg ctgcgcgctg cccttcgcgc
     9001 cgcgccgcgc ctgggtcgag gccgccagcg gcaccgaccg cgtgtggctg ctcggcgaga
     9061 cgcagctggg cctcgccgtg ggggcgcccc tgccgcagcc ctaccgcggc cgccccgagc
     9121 gcttcgagcc gctgcagacc aatcccgatc cgctgcgcct ggcgtggacg ctggccctgc
     9181 cgccccacgg cgggctgctc gggctgtgca ccgacgaaga ccatctcttc gtgctcggcg
     9241 aaacccccga cagcacggcg gacgccccgc gcatgcagat cttcatgcgt gcgctcgccg
     9301 ccgcaccggg cgagggcttc gacatccggc gcctgccgga cgggctgccg ctcgcgaccg
     9361 acctcgccgc ggccggcgag ggccggctcc tgctcctgcc gcccatcgac gaaggcgcgg
     9421 aacctggaac gcggcgcgac tgcccgctca tctccttgcg cgaggacgcg cccaccgcag
     9481 agttggtgcc cgagcgctgg ccgcgccgtc ccgccgcagc gctgcccgcc gcggaccgct
     9541 tcgtgcgaca ccgcgacggc cggccgcgcg ccctgagcgc agacggcccg gtccggctct
     9601 accgcctcgc gcaggcccgc ttcgcgccca acggcacggt cacgctgggc acgccgctcg
     9661 actcggcgat gcccgacacg ctgtgggacc gcatcttcat cgacgcctgc atcccccccg
     9721 gctgccggat cgatttcgcc gtgcaggcag gcgacgaccg cgagaacctg cccgcggagt
     9781 ggatcgcgca gccgcagccg gtgctgaccg cggtgtcgtc ggagctaccc ttcgcgtccg
     9841 ggcgcgcacc ccggagtggg gatcacgcag atcgttccgg cctgttcgag ctgctgatcc
     9901 agcgcgccag cggcgcggtg cgcgaggtgc gcgggcgcta cctgcgcctg cgcatcacga
     9961 tgcacggcga cggccgccac agcccggcga tcttcgcgct gcgcgtgcag tacccgcgct
    10021 tctcctggca gacccactac ctccccgagc atttccagca gcaggagcgt ccgctcgcga
    10081 gcgcggaggc caaccaggca gaggcgaacg gcgccgactt ccgcgagcgt ctgctggcga
    10141 gtttcgaggg tctgctgacc ccgatcgagg accgcatcgc cgcagccgag atcctgctcg
    10201 accccgcggt cgcgcccgtg gcgcacctgc ccggcctcgc cgcgatgctc ggcaccacgc
    10261 tgccgcccca ctggccggaa gcacgccgcc ggcgctggct gggcgcgcag ggcatgcttc
    10321 agcagagcca cggcagctac cgcggcctgc tgctcgcgct cgacatcctc accgatggag
    10381 cggtggcgcg gggcgcggtg atcccggtcg agcacttccg cctgcgccgc acgatggcaa
    10441 cgatcctcgg tgtggacatg gatgaccgcg accacccgct gaccctgggc accggtcttt
    10501 ccggcaacag cctcgtcggt gacagcctga tcctgtccga cgacctcgcc cgcgagttcc
    10561 tcgccctctt cgccccggag gttgccgaag cgaagggcga ggccgcggtg gtcgaacgct
    10621 tcttcgaaga agccgcgcgg cgcatgacgg tgatcctgca cggaccggcg cgacggctcg
    10681 cagccgtcgt gcgcgacgcc ctgcccgcgc tcgtgcccgc gaccgtgcaa tgggcgatcc
    10741 gcagcagcga gcaccccttc gtgccgggtc tgtcgccgct gctgggcatc gacacctggc
    10801 tggaagcctc gccaccagcg cggcccgtgg tgctcgaccg cacacggctg ggtcgcggtg
    10861 acctgctgca caacccggtc gccctcgacc ccgagcacgc cgtgccgatc gacgcgaccg
    10921 tcctggacgc accgtgaagg acaacacagg agaagggcca tgagcgagcc cgacgacaag
    10981 cgcgacacga gcctcgccag cgacggccgc ttcgaggaaa tgctgaagcg cgtgaactac
    11041 gcacccggca tgctgctcgg catcgaggcg acgcttgccg aacaggatta ccaccgccgc
    11101 cgcgccaccc gccacggcta ctggctgcat ggcgccggca ccgtcgcagg tctgcgcgtg
    11161 agcctgcagt caaaggaccc cggcaacgac accgaaaacg tgcgcgtgcg cctggtggtg
    11221 agccccggga tcggcgtcga cggcctcgga cgcgagctaa gcgtggccga gccctactgc
    11281 gtcgatctcg gcgcctggct caccacccag cacgaggagc ccgagcgctg gaacgcgctg
    11341 atccgcgacg gctacgccgc agacgacaac ctgctgtggc tcaaggtcac gatgcgctac
    11401 caggactgcg cgagcggcct gcagccggtg ctcgcaaccg agctcaacgc gggcaccgat
    11461 ccggtgcagc cgagccgcgt cgccgactgc gtgctgttcg agctcgtcgc cgagcgcccc
    11521 gacgacgctc cggccgagga gcacctcttt gccgcccacg cgcgcatccg cccctacgac
    11581 gagatcgagg acaagctggg cgagcgcgaa cgcgcccagg tcgaggctgc caccggcggc
    11641 gcgcgcgcgc agctcgagct cggcgcccgc ctgctgcatt cgctcggcga cgacaaccgg
    11701 gccctggtcg cacgccaggc cttcctgacc gacccggccg cactcgctcg caccctgctc
    11761 gcgcgcattg cgatccgcct cgcgccgccc gcgcccgatc ccgacctgat cgtcaatccg
    11821 cgccgaatcg agatcgacaa cctcgtgcgc cccttcctct tcaacgccgt cgcgcttgct
    11881 cgcctgatcc gcgcctaagg aaccgccatg gccaacatcc acttcacccc gctccggatc
    11941 ggcacgagca acgccgccgg cgagcgcatc tccagcgtcg atcccctgct gtcgcgcacg
    12001 aactacttcg acggccagct cctgaaggcg gccgacctcg cacgcgacca gatctacctc
    12061 gacgaacgcc tgctcgagct cggccaggtc ttcggcgccg gcatcgtgcg cggcctcgaa
    12121 cccagcctgc attcgggcca cctgctggaa gtctctcccg ggatcgcgat cgcgccctcg
    12181 ggccgcgtgc tgcaactctc gaacaccgcg ctgaggatcg acctgctcga cagcggcctg
    12241 atcgcgaccc tcaacgacgg ccgcttccgc ggccccgcac gcggcctaca cgccctcgcg
    12301 ctgacccaca cccaggtcgt cgatggcgtc gccgaggcct tccccaagga cctctccacc
    12361 gcgcgggttc cccatgtcgc ggcatgggcc gagggcgtcg agctgcgcct gatgccgctg
    12421 ccgctgccgc tgccacgcca ggacgagatc gccgtgcgcg ccagcctcgc gcgcgagctg
    12481 ctctccggtg gcgagcgcct cgcgctgccc tcggacgacg ccgtcgcgct cggcctcatc
    12541 gcggtcgagc gcggacggat cctgtggctc gaccgcggcc tgctgcgccg gccgcagcgc
    12601 ccgccgaaca cccccgacgc gatccagcag gacctcgcca cgcattatca ggagctgatg
    12661 caggccgtgc tcgcggcgcg ccgcagcgcc ggcctgcgcg gagccttcgc ggccggccag
    12721 tatttccgcg tgctcccccc ctgggggccg ctgccgcagg aagcgatcga cccggtcggc
    12781 ggccgccagc agttcttccc gcaggattac gaggtcgcca tcgccccggt gcggcgcagc
    12841 gagctcgccg ccgtgctcgc cgactccgcc cggctcgcac cgatcgacct cgaacgcgac
    12901 gccgactgcg acgtgatggt gctcgtgccg atgagcgaca gcgccttcgc gctgcgcgcc
    12961 cgccagctcg aggcaccggc cgagttccgc ccgcgcgccc tcgggcggct cgccaacctc
    13021 gaccgcctcg ccctgcgcat cctcccgcgc ccgccgatcc accgcgtgga caccgacgcg
    13081 gacgtgtggc gcgcgatctg ggccgaagcg gatccggccg agctgatgta cgtacgccgc
    13141 ccgccgcgca ccgcggaaac cggggtgagc gcgctggtac tcgccctcgg ggcgagcctg
    13201 ccgccgcccg gcagcgcgct gccgcccgac gtcgcagcac tcgaggaacg gctcgatgcg
    13261 gccctcgaag cggtcgacgc cgccgcgcag gcgcgcacca cgctcgagaa cgaggcggcg
    13321 cgcctgaggc tgcgcatcgc aaagctcgag aaggccctcg ccgcaaccgg caccggcgta
    13381 cctgacgacc tgctcgcgga ggtggagcgc ctcgggctcg cgctcgagac ggcccgcaag
    13441 gagatcgccg cgctcgaggc cgaagaccgc aacgccgcca tcctcgccaa cgcgctcgag
    13501 gcggcaagcg accggatcga cgcgctgaag ctcgcgctcg acgccgccaa tgccgagatc
    13561 gagcgcctga agaccgccag cgccccggtc gatgagaccc tcgaggcgcg catcgaggag
    13621 ctcgtcgccg cactcggcag cgagcgcacc cgggcggcca cactggaaca ggaacgcacc
    13681 gcgctctcca cccggctcgc ggtcgcggag gccaccatcg gcaggctcga gcgcgaggct
    13741 gcgcaagccg gcgacctcgc cacccagctt cccctcgcgc tgctcgcaca ggcccgcggc
    13801 gcagacgacg ccggcatcaa ggccgcgacc ggcgccgaca agctgatcgg cgccgacgcg
    13861 cgggcgcgca tcgccgcagt gcagatcgtg ggcatggcgg accgccgcct cgaccccgcg
    13921 ctgtggccga ccctgccagc gctggcgcga ctcgaccccg ccgtcttcgt gaagcttcgc
    13981 gaccacttcc tcgccgcggc cggccagctc gatcttgcga agttcttcct cgaggaaggc
    14041 cccggcctcg gtctttccgg tgcgctcctc gaactctgga aacgcgtcct cggctgacga
    14101 ccatggacac tccgggcgtc gcatcccgaa cccacacgag gcgttgagca atcatggcca
    14161 ccctgtcctt catcccgccc ggcctcctcg ccgcgctcgc gtcgcgaccg gccgcggccc
    14221 tgctgccccg cacgccaccg tccatccgcc tgcacgagcc gcagcgacgc ccggacgacc
    14281 cgcgccggat caaccttttc cccgggcggg cgctcggcga gcgtgagttc gagcgcctgc
    14341 aggcctacgt cgacgatcgc atcgcaccgt tgctggcgag cctcgcgcca ggcatcgtcg
    14401 agggcctggc gtgcgcgctc ttcggcaccg gcaccgacac ccgcgtacgc gtcgggcctg
    14461 gcctcgccgt cggcgcggcc ggccagctcg tacgcctgtt ctacccgctc gaccaggcat
    14521 ggagcgagct cgtcgcccag gccgagcgcg accaggacgc cccgctgcgc gacggcctct
    14581 tcctgctcac catccgcagc gcggtcgaag agatcgacga cgcgaccgca gcggaaccct
    14641 gcacccgcag cgaaccggat gcgctgcgcg agcgccgcat cgagacggtc agcctcctgg
    14701 gcctgcagcg catcgcgggc agcccgcgca tgatggcgat gccgcaggcg cgcgccgcca
    14761 accgcatcgg cgtccgcctg ctgcgcgaat cgcccttccg cgcggactcg gggggcgtgc
    14821 cgctcggact cgcgaagatc gtcggcaagc tgccggtctg gttcgacgcg tgcgccggac
    14881 gcctgctcgc cgagccggat gcggccgccc atagcctgct cgcccatgcc gtcgccacgc
    14941 tcgaggcctg gcagcgcgcg catcccgccc ccctccccgc ggtggcgacg cagacgctcg
    15001 acgaactcct cgggctcgat ttccttcccg ctgccggccc gctcccggcc gcgctgctgc
    15061 gcgaccccgc cgcgcggagc ccgagcctcg ccttcttgcc tggcgacctg cgcgtcgagc
    15121 tcgcgccggt gccggcgggc accgtggagg agggggtcgt cggcgagctc gggcgcggtc
    15181 ccgtcgacct gatccacggg ctcggcgaac atctccgcct gttgctggca atccccgatc
    15241 tcgactaccg ccgcgacctc ttcgacctgc cacagcgcga cacccggctc gaagacgaac
    15301 tgttccgccg cggcgaacgc gcggcgcacg cctaccaagc gtggaaggca cggtggtatg
    15361 cgctcttcca cggtctgagc ggcgcccagc tggaggcgct gcgcgcgccg gtgctgctca
    15421 ccaaggctcc ccccgagccc gggcaatacc gcacgaagct ggtcaccgac cgcgcgaaga
    15481 cgctgcggcc cgctccgcag ggcacgacgt cccatcccct cgcggacctc gccacggagg
    15541 gcacggcagc gctgcccgaa ccctacgcca gccacctcgc cgacccccac cccgccccgg
    15601 acgactacca gcccgtcccc gacatccagc ccgccggcaa cggcctgctg caccggcgcg
    15661 aggcgctgcg cgccgacatc cgccacctcg aggccgcgct cgacaagagc ttccgcctgc
    15721 tcgaggaggt cggcgacttc ctcggcctgc agcgccagca actcgacgcg ctgacggtgt
    15781 cgttcagcac gcttgcgggc ggcgtaccgg gcgacggcct cggttcccag ctcgtcaggt
    15841 ggacggcgaa ggcgaacttc gtgcccaagc cccccccgga gaacccgtca tgagcgacat
    15901 cctcaagcgc ttcaccaccg acgcgagcgc attcaccgaa ctcaagttcg tggacgcgaa
    15961 gggcacggca gccgcggtcg atctcgccga cgcgacacgg ggcgaggcca cgatctccgc
    16021 cggcatgctg gccgccctcg ccgacctgca gccgaaggcc gcgcccgacg agccgctcac
    16081 cgagccggtc ttcagcaagg caacgagcac aggcagggga atgctggcgc atgtcgagcg
    16141 agtgcacgac agcttcctca acgtgcgccg cgacctcctc gagctgcgcg ccagggccaa
    16201 cgctcgccag aatcaggcga aatcgctcat cgaccgcgtg tgcgacttcc tcgacgacta
    16261 tctcaagccg gaagagctgc gccggctcgg cgtcatcgac gacgaggccc aggcacgtcc
    16321 cttccgcctg ctcgtcgcgc ccgatctgca caacgccgag ggcgtgctgc tgcgcccccg
    16381 actcgacctc gcccttcccc gcccgggcga ggacgacatc gaaggcgtca agcacaacga
    16441 cgaatcggcg ctcttccatg ccggcaaggt cgttgccgac gacatccgcc aggtcgaggc
    16501 gctgcgcggc gccctgacca cgctgcgccg ccgccatcag gaggcgctcg aggacctgcg
    16561 cacccgcctg gtcgcactcg aggccgagct acccggcgag cttcgccggc tcgacgtcct
    16621 ggagcgcgaa cgcaccgaga ccctggacga ctacgcggtt gcacaacgcc tgctggccga
    16681 acactggcgc gaggtcgaag ccgcccacgc cgagcgccgg cgaatcatcg aggctcatca
    16741 aggcctcttc tacgtgaagg tgcgcgagac cccgctcggc cgcagcctgc ccgacccgct
    16801 cgagctgcgc ccgagcagcc ccgacgagct cgtgcccggc tgcgcaggac gcgacaccgc
    16861 gctgccggct gcgctcgcgc ctttcatcga ggcggtgtac gacatcccgg cggccgattg
    16921 ggcacacctg cggccactcg gccacctgct gccggggcgc acgatcctcg ccggcctggt
    16981 cgagatgcgc cgccagaagc tcgcactgcg cctgaaccgc cccccggacg ccggcctcgc
    17041 cttgctgtcc ggcctggtgc agcagaacaa ggccctggtg cgcgacatcg ccgcgcgccc
    17101 cttcagcgcc ggcgcactcg gcgagttgca gcgccaggca ggcgcgatcc tcgccctcga
    17161 cgacctgctc gcgatcccct cgccacagct gcgcgacccg gcgcgtgccc tgcaccagcg
    17221 cctggatacc gccgccggct gcctgctcga gcgtctgcgc acgatctctc cctcgatccg
    17281 gctgaactgg gcgagcgcgg ccgaggctga ccggctcgcc gtcgaggccc ccgagcgctg
    17341 gcccggcctc gccgaggcgg aggagcgcga cttcaacggc gtgcgcacgc tggtcgagct
    17401 cgtcgcctgg tggttccgcc agctcgatgc cgacgcctcc gccgccgcgc acggcgcgat
    17461 gcgcaacctg gtgcgcgcct gcctgctgct cgcggccagc gacgacccgc aacagctcgt
    17521 gcagggccgc ctgcagagca tcccggggcg cttccgcctc ggcgaggcgc tgcgcctgaa
    17581 gctcgaccgc gaggccgcgc ccggcaccct gctccagctc ttcgacgacg accagcgcgt
    17641 gatcgcgacc ctgcgtgtcg acgaccatga cgaccagggc accgtcgcct cggtcgccgc
    17701 catcctcgac cccgagctcg aacgcaaccc cggcgccgtg ctcgccacgg gactgcacat
    17761 cagcggagtc gatcgaggct gagcccgatg atcgcgctcg ccgacctcca catcggtcgc
    17821 ctgacgctgc gcggcagcgc cacggcgatc gcgcacgcac gcgcgaccct gccgcccgca
    17881 ctcgcccgcg cacgctggcc agacgaggaa gcgatcctcg tcgtgcggcg catcgccgtc
    17941 ggcggcactg gcaccgagct gcccgcgcgc gccgccgcgc ttgccggaca gcttgccggc
    18001 cgggcggtcg atccgtggac gccggccgcc gatggcgccg aggtggtgcg ctttcgcgac
    18061 gcacgcgact accgcgcctg cctcgttcgc gacctcctcg ccggcaccgc cgcacgccgc
    18121 tggctatggc gtcatcgagc ggcccttctc gcccacccca ccgcgaccgc gcttgccgag
    18181 ctgctcggcg aggatgccct cgcactcccc gccctgctcg agcacccgcc gctgcgcgcg
    18241 tgcctgccga ccctgtggtg ccgtctcgat gccgccgcgg cacgcagcct cctgcgcgcg
    18301 atcggggccg cgaccggctg gcacggcgcc atctcggacg cgctcgcttc tcgtgccgcg
    18361 gaggcggcag ggagcggcgc ggacctcatg acggaggccc aaacccacca tgagcgcata
    18421 tccggagacg aaatccgccc aggccccgag cccggcccgt cgcccccgcc caccgggccg
    18481 gcggcgacaa gggcgggttc gccgcgccgg ccagccccgc ccggcgcggc ggcgcatggc
    18541 acgctgcccg ccagcgatcc gctcgtcgtg ctggaggccc tgctcacctt gtggcgctgt
    18601 acccccgcag cgctggcggg accgcagggg ggcgaggtcc tgcgcggtgc ggccgcagcc
    18661 ctggcggaac tcggcggcgg gcaggatgcg gagtcggcga cggcacgggc atccgggcct
    18721 ggtcgcgcac gcgctgcagg cggagaccta cccgacgcca gacggcggca cgacgccggt
    18781 gcaggttcga tacccgccgg ctcccccgct tcccgagggt tcgcaccccc cgcctccatc
    18841 cgcgtcggag cccctccccg cgccatcgct gcgataaacg cgcgccccgc cgctccgctc
    18901 cgggcaggag ccccgagcga agttaccgac ccgttcgcgg cgacggtgac ggccacgcag
    18961 gccgaggtcg acttccacac cggccgcggc gggctttttt tcctgctcaa cgtgctgaac
    19021 catccgggat tgcgcgcctg gcggggcacc ctgcacgaac cccacgccgg ctggcgcgaa
    19081 ctcgtccgtc tcgcgctccg gctcgagctc gctcccgacg ccccgctcgc cggcttcctg
    19141 gccgaggcct gcgcactgga gggcgagcaa gaccccgtcg ccaagcttgc gggccttgcg
    19201 aagctgcccg cgcgcgccga tcccgtcgcg gtcgaccggg ccgtgctgcg tcacgtcggt
    19261 gacgcggccc tgcgcgcgct gcgcgccgag cgccccgctc gcgtgcgcgc aagtccgagc
    19321 catgtcgacg tccacctgcg cctcgccgag gtcgacctgg agctgcgtcg gggcggcctc
    19381 gacctcgacc ccggctggct gccctggctc gggcgggtgg tgcgctttca ttacgaccgc
    19441 agcgatgccg ccgatcgcgg aggatcctca ccatgaacgc ccccgccagc ctgcgcccgg
    19501 aatccgcccc ggcaagcgag ggcgcgcgcc tgtcgcgcgc cttcgtccat gccggcatcg
    19561 ccgagttcgc ccgcctccgc gtcggcgccc cgtccgcggt cttcgaggcg cttgcggcga
    19621 gccagggggg cgaagacctc gccaccctgc tcggtacccc gcctcaggac tggcgagccg
    19681 cctgcatcgc actcgcgcaa gccgcgccgg agctgcacgg ccccctcggg cgcctgcttg
    19741 ccgacctgaa gctgcagctg cacgagtggt tcgccatggc gctgtgcggc gagaacgagt
    19801 cgagctacct gctcaacctg gcgctcgccg agttgcagtc gcccggtgcg ctccgccccg
    19861 gccttcatct cctctccgcc gcgagcgcgg ccttgttcgg cgctccgctc gcgcccctcg
    19921 cgctgcccaa ccaccgcctc gtgcgggcgg gggtcctcga gatcgtgggc gaggggccga
    19981 tgcccacccg cagcctggcg gttgcccccg agctgtgggc gctgctctgc ggcgacccca
    20041 ccgcgtggcg cgacaccgcg ccgctcgccg atggcgacac catgctgccg cgcggcttcc
    20101 gcgaacagat ccccacgctc gcgaccatgc tgcgcagcgg cgccacccgc cacgtgatgt
    20161 tccgcggcgc acgcgcggcc ggacttgccg ccgccgcgcg cctggcagcg gcggcgggac
    20221 gcccgccg
//
LOCUS       DAOQNN010000015        19774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_999_length_19774_cov_10.723921, whole genome shotgun sequence.
ACCESSION   DAOQNN010000015 DAOQNN010000000
VERSION     DAOQNN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 19774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19774
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_999_length_19774_cov_10.723921"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PKV97_02095"
     CDS             <1..795
                     /locus_tag="PKV97_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNB04695.1"
                     /translation="AKTLSMDMRANDRGWLKLKLDTLARTVGERSFEMPFPPHGEKKL
                     VPGVVSAFAQVVNYRCEKLGVFDKEDESNGVLDTLFSLEEPKTGTDGTLSWTVDIYNP
                     ATGEDFVLGLKEITLPDGVTRPYSVWLSGNYPRALDGLTRILSLDMRVMDPAWIGMKL
                     RKLLDYPEPLGDFMAFVPGTRRQQNWPSTVAYLAQLIIHRYAMLGVLDERGYPTREMG
                     ILESPRDDNEPKLMQGALCNECGNHTVIRKDGCDFCTACGAVGTCG"
     gene            982..1221
                     /locus_tag="PKV97_02100"
     CDS             982..1221
                     /locus_tag="PKV97_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04696.1"
                     /translation="MPTCPRCDGTDCRESPWKSADERLAHPGERAWRCMSCVHRFHAP
                     EPRRLLRDNPIVAAVGGSTLLLTIAVIAILWFWKS"
     gene            1235..2011
                     /locus_tag="PKV97_02105"
     CDS             1235..2011
                     /locus_tag="PKV97_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HNB04697.1"
                     /translation="MSTRAHPACPACGSTDCRRSRWRSHEEHAGHPGLHPWRCLACDQ
                     RFLAPDDALRPRSRWPLVVGILSALLLLGIGIASVRMLDLDESGDAPGETRSAAAEPS
                     APTDALDARFRAARAALLDTARGREVSASAVSQLREAAEGGHTGAMVLLGKLYRSGIG
                     MPQNYELAARWLNQAAHAGDPEGMVEFGRLHRSGIGVERDAVQAYVWFNRAAALLNMD
                     GVQERDSIAVKLDADELRRAQALSLEAAEEAARQAAERSR"
     gene            2079..3674
                     /locus_tag="PKV97_02110"
     CDS             2079..3674
                     /locus_tag="PKV97_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB04698.1"
                     /translation="MPIPYPPLQPDTAAGPARLVLQGISKAYPTVVANHDIGLVVRAG
                     EIRAILGENGAGKSTLMKIIYGVTRPSSGRMFWEGEEVAVASPAHARSLGIGMVFQHF
                     SLFETLTVVENVALALPGKPALDALARRIVEVSERYGLPVDPRRHIHALSVGERQRVE
                     IIRCLLQDPRLLIMDEPTSVLTPQAVRKLFETLRRLADEGCSILYISHKLDEIRTLCH
                     TATVLRGGKVTGECIPAQETPASMARLMIGKELPRCEHDARQAGSEVRLRLAGLSHAA
                     DDPFGTDLRDLHLDVHAGEIVGIAGVSGNGQQELLRVISGEAPLAEKFPVQICGVEAG
                     RLSPGRRRRLGMAFVPEERLGRGAVPTMSLADNALLTAAHNQGMVAAGFLRFGRARAF
                     ARRCIDEFDVRCGGTAAAAKSLSGGNLQKFIVGREILQAPRLLVCAQPTWGVDVGAAA
                     FIRQRIIDLRNRGCAVLVISEELDELFEICDRIAVIAKGRLSPVRAARDTSIEDIGVW
                     MSGLWPGAEHTAGAPLATETPHA"
     gene            3667..4731
                     /locus_tag="PKV97_02115"
     CDS             3667..4731
                     /locus_tag="PKV97_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB04699.1"
                     /translation="MLELEARAEPSRLMSWASPLLAAALMLVGGLAVFAALGKDPVEG
                     FRVFFLNPVKDLYALSELLLKATPLMLCAIGLAIGFRANVWNIGAEGQFMLGAVAATG
                     VALYFGDAESALVLPAMVLAGAAGGAAWAAIPALLRTRFNANEILVSLMLVYVAQLFV
                     SWLVFGPWKDPDGFNFPQTRMFAEAATLPMVIEGTRLHFGFAIALAALVAGYAFMNRS
                     HAGFRMRVAGEAADAARYAGFSATRMIWVGLLLGGAAAGLAGMGEVAGPMGQLTDKVG
                     SGYGFAAIIVAFVGRLNAFGILLASLLMALLYIGGEQAQQYLNLPNSISMVFQGMLLF
                     FLLGADVFIHYRLRLRRRGT"
     gene            4742..5674
                     /locus_tag="PKV97_02120"
     CDS             4742..5674
                     /locus_tag="PKV97_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB04700.1"
                     /translation="MDASLFTSMLFATVVAGTPLIIVALGLLVNEKAGVLNLGAEGMM
                     AMGAVAAFAVTHESGNPWLGVAAGMGAGMAMAALFGLLVLTLMANQVASGLALAIFGV
                     GLAAFVGKPYEAVALQAVPPIRIPFIADLPLIGEALYNQQALVYFSWALFWGLLWFLH
                     RSRAGLVLRAVGESPASAHAIGYPVIRIRYLAVLFGGAMAGIGGAFLSVFYTPMWAEG
                     MVAGRGWIALALVVFATWRPLRVMVGAYLFGGVMVTQLFIQGSGLQIDFPSQLLSSLP
                     YWATIVVLVAISRNGNTIRLNSPVSLGKPYRPEA"
     gene            5836..8151
                     /locus_tag="PKV97_02125"
     CDS             5836..8151
                     /locus_tag="PKV97_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB04701.1"
                     /translation="MILDLLQATALLLALCLLQGGLARRCAHGGPAFAVGSGLIYGLA
                     CIAGMMMPITLAPGVIVDPRSVVLSVGALFGGPLAGAVAGLAAAAYRIWLGGSGVFIG
                     VTTITLAVLLGLAFRLGAARGWLRPAPLQLFLFGVFTHATSLLLIATVPNQSLAGLFA
                     NLGLPYLLLMGAATLLLGALLRDLEQRRATECALAASEARLRAITAAIPDLVFVVDED
                     GRYLEVITRQENLLAAPPEQVIGRRLEEVLPAAPAALLRAALEQALASPERTCARYAL
                     DTPAGHREFQAWIQAIDAPIGGRRAAAILVQDITANVAAEERIRKLAYYDPLTTLPNR
                     RLLVERLGHAIATNARTGLHGALMFIDLDDFKDINDLLGHHNGDVLLREAGARLRGVV
                     RESDTVARFGGDEFVVLLELLAESADEALLAARQVADKLLEQLGHPYRLDGLDRKCTA
                     SLGLVIFNGHEHTADELMQRADLSMYEAKRSGKNTARVFDPHMRTELADRLALETELR
                     GAIGTGAFLLHLQPQLDAGGRLIGAEALLRWNREGHGPTPPALFIPIAEQAGLMPALG
                     REAMALACEELARWVALPGADGLVMAVNVSAAQLYEPGFVEEVLALLARTGAPAERLK
                     LELTESLLLTDIERAIEAMTRLREMGIRFSIDDFGTGYSSLGYLQRLPLDELKIDRTF
                     VRDLPANASSLAIVRTIMALADTLRIRVVAEGVETSAQHACLLDNGCDAFQGFLFARP
                     EAVESFRARMSATDALHGSGAQPQGGADRDR"
     gene            8208..11372
                     /locus_tag="PKV97_02130"
     CDS             8208..11372
                     /locus_tag="PKV97_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB04702.1"
                     /translation="MSPSAAAIELFIEACPFAVIEWGRDMRVLRWSPEAERVFGWSAA
                     EVLGRRPNEWSFTHPDDAAAVKRAIGQAITADGPTPPVIGRNFTRDGRLLHCEWHNRA
                     NRDPQGRLVSLLSFAKDLTRQLEAERARDLSEARYAHIFNNSHAVMLILDPESGRILD
                     ANPAAEEFYGWSRETLQTMQIGDINTLSPQALLVELKAAHAEERKHFEFRHRRADGSV
                     RDVEVHSGPTEDGNRSMVFSIVHDITERKQAEALSRRWERFFRLSNLGLAMHDVSDNT
                     IIDVNATYASQHGYSIEELRGMRIDELYPEDEREQLHAHLAEADRIGNASFETVHLRK
                     DGSRLPLVIGVTALLDDRGRAVARFAFGLDISARKAAEDELRKLSRAVEESPESIVIT
                     NTRAEIEYVNQAFIDKTGYSRAEAIGQNPRLLQSGRTTPATYADLWNTLTHGRSWQGE
                     FFNRRKDGSEYLERVTITPIHDESGHITHYVAVKQDITAQRRMEEELLRYHEHLEGLV
                     ESRTAELQHALDAANIASRAKSEFLTTMSHEIRTPMNGVIGLLDVLSHSQLSTEQVEM
                     VGIMRESAETLLRLIDDILDFSRIESGNLELDVGPASIPDLIARVVGILQTVANRKSV
                     RLSTRIDPDVPAVVRTDALRLQQILCNLVGNAVKFSSGLDRPGRVEIRVETAGAGRIR
                     FMVTDNGIGIAPEAIEKIFDPFSQAESSTTRRFGGSGLGLSICTRLVRMMKGRMEVNS
                     LPGRGSRFVVTLPLAATNGSATRSEPARSTALARPSPTPPLATPGAGEAGRRILVAED
                     NDINRRVIARQLALLGLQCDTAEDGFEALERWRQGQYSLLLTDLHMPGMDGYELTARI
                     RSEEAPGRRTPIVAVTANALRGEKERCIDAGMDDFILKPVQVAALQEVLARWLQPDTE
                     QRTPAGTAPAAPAAPPAVFDPQALPGLIGNDAALIAEFLGEYRLSACDTVRSIRKACE
                     DGDWRRAGELAHRLKSSSRSVGAMQLGEICAALEQAGRDDDGDQVQRQGGLLEAALAA
                     TLTAMQGTQAAGVPPARGDTPRPG"
     gene            11411..12643
                     /locus_tag="PKV97_02135"
     CDS             11411..12643
                     /locus_tag="PKV97_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB04703.1"
                     /translation="MNLETLKVLVVTDVAADAAQIARLLADHFEHIETSTIESRFIAD
                     FERVQPDVVVLAFTSLERAERYSLGLYRHSEVVHSLPHRSIVLCDKDNVHRAFELCRK
                     EYFDDYVLYWPMVFDTPRLAMSVLIAGRSLQAQRDTPNAREVAAHARSISQLETVLDD
                     RIEVGRQHADALERALQAAEIEASSVQPRPAAPASPSSDSATIPDAGIDALPAFTDSF
                     ELDVSDVPSGLDRPTQFAVTPQEDVIQEAKLRVDEAKKKVVPLTEWIGGLKADVKPQL
                     DAARQLGELAGRTPPLIVVVDDDAFQCKLLERLLGNSGYRSIIAHSGAEALAVLGRQP
                     PDLILMDVALPDFNGVELTRRLKAIPRFAAIPVVMITGHSERQVLEASLKAGAVDFLV
                     KPFDREILLQKLARHLAP"
     gene            12783..14573
                     /locus_tag="PKV97_02140"
     CDS             12783..14573
                     /locus_tag="PKV97_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNB04704.1"
                     /translation="MITEMEIFPWDSNFATGIAVIDAQHQQLVALLNRLVTHLAFQSD
                     APTLNSVFDQLRDYVEVHFTTEEALWQAHLGEYAGVAGHHESHVRFIDEVLRLKAEES
                     TKPFETVIENIVNFLTRWLALHILEADRRMALVVHGVQRGLTLEQARTQADETMSGSM
                     RVLVETVLVMYDKLANRTMAMTREINRRRQAEAELQKAHEALKRAKENAESVSEAKSS
                     FLASMSHEIRTPLAAILGMSHLMHQEELPASQRERLDVIENAGRHLLSIINGILDLSK
                     IEAGKFTLDEAALDLRGVVEEALAMLAHQAEAKGLSLRAEFDLPPGTFLGDATRLRQA
                     LVNYLINALKFTERGGITVRCSASAVDTDAVDVRFVVEDTGPGIAAAELRRLFKPFTQ
                     TEATSTGEHGGTGLGLAITARLAELMGGQAGAESEPGRGSRFWFTTRLRKVKADEVVA
                     PTGSPLEIRSALAERHGGACVLLADDDKVNREIARVLLSRGGLAPECAADGEEAAALA
                     ERCKPALILMDMQMPVVDGIAATRRIRTQDWGREVPIIALTANAFAEDRVRCLEAGMD
                     DFLTKPIMPAVLYSKLLYWLDRSRRERARS"
     gene            complement(14596..15216)
                     /locus_tag="PKV97_02145"
     CDS             complement(14596..15216)
                     /locus_tag="PKV97_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNB04705.1"
                     /translation="MKLYVSPRAPNPRRVQMFIAEKGIRGIEEVAVDLVKGEHRSSDH
                     RARSPLAKVPVLELDDGRYLGETRAICSYLEGLYPEPNLMGESFEERAFVEMTDRRVE
                     LYLFGGIMNCVRHTHPGLAPLEQPQFEDFGRSQGEKVREVARWLDEELGRQPFVAGER
                     FTIADITAFCAIEFARGLMRFRPGAEGMANLQDWRDRIAERPSARV"
     gene            complement(15261..15971)
                     /locus_tag="PKV97_02150"
     CDS             complement(15261..15971)
                     /locus_tag="PKV97_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNB04706.1"
                     /translation="MIDLYTAATPNGHKVSIALEELGLAYSLRVLDLSANEQKEPWFL
                     AINPNGRIPAIVDHDEGDFAVFESGAILIYLAEKTGRLMPQDARGRSRVLQWLMFQMG
                     GIGPMMGQANVFFRYFPEKIQPAIDRYQGEVKRLFRVLDGHLAKNEYLAGDYSIADIA
                     NWAWVRTHNWSGVAIDDLPDLARWRDQIRARPAVQRGIEMPPSSFDRDGDGEEQAKAF
                     SAKVRNMVETGQSRSGGV"
     gene            16248..16721
                     /locus_tag="PKV97_02155"
     CDS             16248..16721
                     /locus_tag="PKV97_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB04707.1"
                     /translation="MSYEIRSILYASDLTPRSPAVFRHAVGLAMKFNAKLHAVTVTLP
                     SSSLPYEEFLSEEKMDEIKLAGHKKAEALLRHRIDVFAEANPELDVKSVLASVRALEG
                     DPSRRILDVAKHVLADVIVMGSRGHSTIGELLLGSVAHKVTMKADIPVMLVPIDR"
     assembly_gap    16835..16934
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16968..17906)
                     /locus_tag="PKV97_02160"
     CDS             complement(16968..17906)
                     /locus_tag="PKV97_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB04708.1"
                     /translation="MSLRRSDDPLDTYLLRVFVLLISERSVSRTALRLNQSQPAVSAA
                     LKRLREILGDPLLVREKGGMVPTERALALMSHAKGALAEIDRMTDAPESFEPHSTRHE
                     FRVGSPDYLAPVFVASVAERFRREAPQARLSLHALGPSFDFERSLAEGDLDVVIGNWP
                     EPPHRMHLSMLLEDEIVCLVAASHPCAKKGMSVDDYLRAAHVVPMPYSISQRGVIDSV
                     LASMRINRDERVAVQSFTAAPYLLQGTDLVFTTTRHFAQFYANLLPLAIIPSPIAFPP
                     MRFYQLWHERNHHSTGHRWLRRLLSDCGRRIAPPHA"
     gene            18085..18435
                     /gene="uraH"
                     /locus_tag="PKV97_02165"
     CDS             18085..18435
                     /gene="uraH"
                     /locus_tag="PKV97_02165"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HNB04709.1"
                     /translation="MGRLTTHVLDTAHGKPGTGITVTVYRLDGERREVARSVTNHDGR
                     CDKPLLEGAALEAGKYEIVFAAGDYFRALGLNLPEPPFVDEVALRFGIADVAEHYHVP
                     LLVSPWSYSTYRGS"
     gene            18489..19682
                     /locus_tag="PKV97_02170"
     CDS             18489..19682
                     /locus_tag="PKV97_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HNB04710.1"
                     /translation="MEAYLLDWANLLVRWLHLIAGIAWIGASFYFVMLDNSLKPPKNP
                     NDAKRGVFGELWAVHGGGFYHSQKYLTGPKGEPLTQDLHWSKWEAYTTWLSGMGMMAV
                     IYWIGASSYLIDSSVMALSPAAAVGISIAFLVAGWVFYDLLCRNMIGKDGVLAAIVFV
                     FVMVANYVLHQVFSARGAYIHVGAMLGTMMAANVFFHIIPGQKKMVEQIRRGEQVDPM
                     PGTVGKQRSVHNTYFTLPVLFIMISNHYPMTYANAKGWLVLGVLMLAGVLIRQFFVLR
                     HRGQVKWWLPAAGVALIALLMVLMAPKQVDAGGEKVSFATVKAIMAQRCIACHADKPT
                     FEGFAQAPKGVMLQTPEQIAAHAVMSAQTVASGYMPLGNLTAITEEEREKIAIWFAQG
                     ARTAD"
BASE COUNT         3107 a         7114 c         6479 g         2974 t
ORIGIN      
        1 gccaagaccc tgtcgatgga catgcgcgcc aacgaccgcg gctggctcaa gctcaagctc
       61 gacaccctgg cccgcaccgt gggcgagcgc tccttcgaga tgcccttccc gccgcacggc
      121 gagaagaagc tggtgcccgg cgtggtgtcg gccttcgccc aggtggtgaa ttaccgctgc
      181 gagaagctcg gcgtgttcga caaggaagac gagagcaacg gcgtcctcga cacgctgttc
      241 agcctggaag aacccaagac cggcaccgac ggcacgttgt cgtggacggt ggacatctac
      301 aacccggcca ccggcgaaga cttcgtgctc ggcctcaagg aaatcaccct gccggacggc
      361 gtcacccgcc cgtactcggt gtggctgtcg ggcaattatc cgcgcgcgct ggacggcctg
      421 acccgcatcc tgtcgctcga catgcgcgtc atggacccgg cctggatcgg catgaagctg
      481 cgcaagctcc tcgactaccc cgagccgctc ggcgacttca tggccttcgt gcccggcacc
      541 cgccgccagc agaactggcc cagcaccgtg gcctacctgg cgcagctcat catccaccgc
      601 tacgccatgc tcggcgtgct cgacgagcgc ggctacccga cgcgcgagat gggcatcctg
      661 gagtcgccgc gcgacgacaa cgagcccaag ctgatgcagg gcgcgctgtg caacgaatgc
      721 ggcaaccaca ccgtgatccg caaggacggc tgcgacttct gcaccgcctg cggcgcggtc
      781 gggacctgcg gctgagcgcg cgcgccggca tcgcgcgaac gtcatattca gcaaaaaagg
      841 gcagccctgc gggctgccct tttttgcgct cggtgtgatc cgtcgatcgt ttcttggacg
      901 cagacgctct atataatcga gaaaatcttc agaaacggtg cgcagcccgc cctgcgacca
      961 cggcacgccc gagcgatcac catgcccacc tgcccgcgtt gcgacggaac cgactgccgc
     1021 gagtccccgt ggaaatccgc ggacgagcgc ctcgcccacc cgggcgagcg cgcatggcgc
     1081 tgcatgtcct gcgtgcaccg cttccacgcc ccggagccgc gccgcctgct gcgcgacaac
     1141 ccgatcgtcg ccgcggtggg cggcagcacc ctgctgctga cgatcgccgt catcgcgatc
     1201 ctgtggttct ggaagagctg agcggggagc gcgcatgagc actcgcgccc atccggcctg
     1261 cccggcctgc ggatccaccg actgccggcg ttcgcgctgg cgctcgcacg aggaacacgc
     1321 cggccatccc gggctgcacc cctggcgctg cctggcctgc gaccagcgct tcctggctcc
     1381 cgacgacgcg ctccggccgc gcagcagatg gccgctggtg gtggggatcc tgtcggccct
     1441 gctgctcctg ggcatcggca tcgcgagcgt gcgcatgctg gacctcgacg agtccggcga
     1501 tgcgccgggc gagacccgat ccgccgcggc cgagccctcg gcgccgacgg acgcgctcga
     1561 cgcccgcttc cgcgccgcac gtgccgcgct gctcgacacc gcgcgcgggc gcgaagtgtc
     1621 cgccagcgcg gtgagccagt tgcgcgaggc tgccgaaggc ggccacaccg gtgccatggt
     1681 gctgctcggc aagctctacc gcagcggcat cggcatgccg cagaactacg agctcgccgc
     1741 gcgctggctg aaccaggcgg cgcatgcggg cgatcccgag ggcatggtgg agttcggccg
     1801 cctgcatcgc agcgggatcg gcgtcgaacg ggatgcggtg caggcctatg tgtggttcaa
     1861 tcgcgcagcc gccctgctca acatggacgg cgtgcaggag cgcgacagca tcgcggtcaa
     1921 gctcgacgcg gacgagctcc ggcgcgcgca ggccctgtcg ctcgaagccg ccgaagaggc
     1981 cgcccggcag gcggccgagc gcagccgctg agcccccggg cgtccacgac gccgcacgcg
     2041 cccgctttcc gaccgacgac acaccgcctc gaatcgacgt gcccatcccg tatccccccc
     2101 tgcagccgga caccgccgcc ggccctgccc gactggtcct gcagggcatc agcaaggcct
     2161 acccgacggt ggtcgccaac cacgacatcg gcctcgtcgt gcgcgcgggc gagatccgcg
     2221 ccatcctcgg cgagaacggc gccggcaagt cgacgctgat gaagatcatc tacggcgtca
     2281 cccggccgag cagcgggcgc atgttctggg agggcgagga ggtcgcggtc gccagcccgg
     2341 cgcatgcgcg cagcctgggc atcggcatgg tgttccagca cttctcgctg ttcgagacgc
     2401 tgaccgtggt ggaaaacgtc gccctggccc tgcccggcaa gcccgcgctc gacgcgctcg
     2461 cacggcgcat cgtcgaggtc tcggagcgct acggcctgcc ggtcgatccg cgccgccaca
     2521 tccacgccct gtcggtgggc gagcgccagc gcgtcgagat catccgctgc ctgctccagg
     2581 acccgcgcct gctgatcatg gacgagccca cctcggtgct cacgccgcag gcggtgcgca
     2641 agctcttcga gaccctgcgc cggctcgccg acgaaggctg ctcgatcctc tacatcagcc
     2701 acaagctcga cgagatccgc acgctctgcc acaccgccac cgtgctgcgc ggcggcaagg
     2761 tcaccggcga gtgcatcccg gcgcaggaga cccccgcctc gatggcgcgc ctgatgatcg
     2821 gcaaggaact gccgcgctgc gagcacgacg cgcggcaggc cggcagcgaa gtccgcctgc
     2881 gcctggccgg gctctcgcac gccgcggacg accccttcgg caccgacctg cgcgacctcc
     2941 acctcgacgt ccatgccggc gagatcgtcg gcattgccgg agtctcgggc aacggccagc
     3001 aggagctgct gcgcgtgatc tcgggcgagg cgccgctggc ggagaaattc cccgtgcaga
     3061 tctgcggtgt cgaggccggc cggctgtcgc cggggcggcg gcggcggctc ggcatggcct
     3121 tcgtgcccga ggagcgcctc ggccgcggcg cggtgccgac gatgtcgctc gccgacaacg
     3181 cgctcctcac cgccgcgcac aaccagggca tggtggccgc cggcttcctg cgcttcgggc
     3241 gtgcgcgcgc gttcgcacgg cgctgcatcg acgagttcga cgtccgctgt ggcggcacgg
     3301 ccgccgcggc caagtcgctg tcgggcggca acctgcagaa gttcatcgtc ggccgcgaga
     3361 tcctgcaggc cccgcgcctg ctcgtatgcg cgcagcccac ctggggcgtg gacgtcggcg
     3421 ccgccgcctt catccgccag cggatcatcg acctgcgcaa ccgcggctgc gcggtgctgg
     3481 tgatctcgga ggagctcgac gagctgttcg agatctgcga ccgcatcgcg gtgatcgcca
     3541 agggccggct gtcgccggtg cgcgccgcgc gcgacaccag catcgaggac atcggcgtgt
     3601 ggatgagcgg gctgtggccg ggcgccgagc acactgccgg cgcgcccctc gcgacggaga
     3661 ccccgcatgc ttgagctcga agcccgtgcc gaaccctcgc ggctgatgtc ctgggcctcg
     3721 ccgctgctcg cggcggcact gatgctcgtc ggcggcctgg cggtgttcgc cgcgctcggc
     3781 aaggacccgg tcgaaggctt ccgcgtcttt ttcctcaacc cggtcaagga cctctacgcg
     3841 ctctccgaac tgctgctcaa ggccacgccg ctgatgctgt gcgcgatcgg cctggcgatc
     3901 ggcttccgcg ccaacgtgtg gaacatcggc gccgagggcc agttcatgct cggcgcggtg
     3961 gccgccaccg gcgtggcgct gtacttcggc gacgccgaga gcgccctggt gctgccggca
     4021 atggtcctcg ccggcgccgc gggcggggcg gcttgggcgg cgatcccggc gctgctgcgc
     4081 acgcgcttca acgccaacga gatcctggtg tcgctgatgc tggtctatgt ggcccagctc
     4141 ttcgtgtcct ggctggtgtt cgggccatgg aaggaccccg acggcttcaa cttcccgcag
     4201 acgcggatgt tcgccgaggc ggcgaccctg ccgatggtga tcgagggcac ccgcctgcat
     4261 ttcggcttcg cgatcgcgct cgccgccctg gtggccggct acgccttcat gaaccgcagc
     4321 cacgcgggct ttcgcatgcg ggtcgccggc gaggccgccg acgccgcgcg ctacgccggc
     4381 ttctcggcca cccgcatgat ctgggtgggc ctgctcctcg gcggcgccgc cgccgggctg
     4441 gccggcatgg gcgaggtggc cgggccgatg ggccagctca ccgacaaggt cggcagcggc
     4501 tacggcttcg ccgccatcat cgtcgccttc gtcgggcggc tcaacgcctt cggcatcctg
     4561 ctcgccagcc tgctcatggc cctgctctac atcggcggcg agcaggcgca gcaatacctg
     4621 aacctgccca actcgatctc gatggtgttc cagggcatgc tgctcttctt cctgctcggc
     4681 gccgacgtct tcatccacta ccgcctgcgc ctgcgccggc gtggcacttg aggaggccgc
     4741 catggacgcg agtctcttca cctcgatgct gttcgccacc gtggtggcgg gcacgccgct
     4801 gatcatcgtc gccctcggcc tgctggtgaa cgagaaggcc ggggtgctca acctcggcgc
     4861 cgagggcatg atggcgatgg gcgcggtcgc tgccttcgcc gtcacccacg agagcggcaa
     4921 cccttggctg ggcgtggccg ccggcatggg cgcgggcatg gcgatggcgg cgctcttcgg
     4981 cctgctcgtg ctcaccctga tggccaatca ggtcgcctcc ggcctggccc tggcgatctt
     5041 cggcgtcggc ctggcggcct tcgtcggcaa gccctacgag gcggtggcgc tgcaggcggt
     5101 gccgccgatc cgcatcccct tcatcgccga cctgccgctg atcggcgaag cgctctacaa
     5161 ccagcaggcg ctggtgtatt tctcctgggc gctgttctgg ggcctgctgt ggttcctgca
     5221 ccgcagccgc gccgggctgg tgctgcgcgc ggtgggcgaa tcgcccgcgt cggcgcacgc
     5281 gatcggctac ccggtgatcc gcatccgtta cctcgcggtg ctgttcggcg gcgccatggc
     5341 cggcatcggc ggcgccttcc tgtcggtgtt ctacacgccg atgtgggccg agggcatggt
     5401 cgccgggcgc ggctggatcg cgctcgcgct ggtggtgttc gcgacctggc ggccgctgcg
     5461 ggtgatggtc ggggcctatc tgttcggtgg cgtgatggtc acccagctct tcatccaggg
     5521 ctcggggctg cagatcgact tcccctcgca gctgctgtcg tccctgccct actgggcgac
     5581 catcgtggtg ctggtggcga tctcgcgcaa cggcaacacg atccggctca actcgccggt
     5641 ctcgctcggc aagccctacc ggcccgaggc ctgacgcctt tcgccgcgac cgcgacggct
     5701 cggcagccct ccagcgcggg acaacccatc tcgggtcgcg cgtgacgcag tcggcagcag
     5761 ccgggcggag cgcgggctag catctgcggc atccgaacat gctccggcgc aggtccgcct
     5821 gcccgtaccc gcccgatgat cctcgacctg ctccaggcca ccgccctgct gctcgccctc
     5881 tgcctgctgc agggcggttt ggcacgccgc tgcgcgcacg gcggcccggc attcgcggtc
     5941 gggtccgggc tgatctacgg gctggcctgc atcgccggca tgatgatgcc gatcacgctg
     6001 gcgcccggcg tcatcgtgga tccgcgctcg gtggtgttga gcgtgggcgc actcttcggc
     6061 ggtccgctcg ccggcgccgt ggccggcctg gccgccgcag cctatcgcat ctggctgggc
     6121 ggcagcggcg tcttcatcgg cgtgacgacg atcacgctgg cggtgctgct cggcctcgcc
     6181 ttccgcctcg gcgccgcgcg cggctggctg cggccggcgc cgctgcagct cttcctcttc
     6241 ggcgtattca cccacgccac cagcctgctg ctgatcgcaa cggtccccaa ccagtccctc
     6301 gccggtctct tcgccaacct cggcctgccc tacctgctgc tgatgggggc cgccacgctg
     6361 ctgctcggcg ccctgctgcg cgacctcgag cagcgccgcg cgaccgaatg cgcgctggcg
     6421 gcgagcgagg cgcgcctgcg cgcgatcacc gcggccatcc ccgacctggt cttcgtggtc
     6481 gacgaggacg gccgctacct cgaggtcatc acccgccagg aaaacctgct cgccgcaccg
     6541 cccgagcagg tgatcggtcg acgtctggag gaagtcctgc ccgccgcgcc ggccgcgctg
     6601 ctgcgcgcgg cgctggagca agccctggcc agcccggagc ggacctgcgc ccgctatgcg
     6661 ctcgacacgc ccgccggcca ccgcgaattt caggcctgga tccaggccat cgacgccccg
     6721 atcggcgggc gccgcgcggc ggccatcctg gtgcaggaca tcacggccaa cgtggccgcc
     6781 gaggaacgca tccgcaagct cgcctactac gacccgctca ccacgctgcc caaccgccgc
     6841 ctgctcgtcg agcgcctcgg ccacgcgatc gccaccaatg cgcgcaccgg cctgcacggc
     6901 gcgctgatgt tcatcgacct ggacgacttc aaggacatca acgacctgct cggccaccac
     6961 aacggcgacg tgctgctgcg cgaggccggt gcacgcctgc gcggcgtggt gcgcgaatcc
     7021 gacacggtgg cacgcttcgg cggcgacgag ttcgtggtcc tgctcgagct gctcgccgaa
     7081 tctgccgacg aggccttgct cgccgcgcgt caggtcgcgg acaaactgct cgaacagctc
     7141 ggccatccct accgtctcga cggactcgac cgcaaatgca ccgcgagcct gggcctggtg
     7201 atcttcaacg gccacgagca cacggccgac gaactgatgc agcgcgccga cctgtcgatg
     7261 tacgaggcca agcgctcggg caagaacacc gcccgcgtct tcgatccgca catgcgtacc
     7321 gagctggccg accggctcgc gctggagacc gagctgcgcg gcgcgatcgg caccggcgcg
     7381 ttcctgctcc acctgcagcc ccagctcgat gccggcggac gcctgatcgg cgccgaggcc
     7441 ctgctgcgct ggaaccgcga ggggcacgga ccgaccccgc ccgccctgtt catcccgatc
     7501 gccgagcagg cgggcctgat gcctgcgctc ggacgcgagg cgatggcgct cgcctgcgag
     7561 gaactcgcgc gctgggtcgc gctccccggc gccgacggcc tggtgatggc ggtcaacgtc
     7621 agcgccgccc agctctacga gcccggcttc gtcgaggagg tgctcgcgct gctggcgcgc
     7681 accggcgccc ccgccgagcg gctcaagctc gagctcaccg aatcgctgct gctcaccgac
     7741 atcgagcgcg ccatcgaggc catgacccgc ctgcgcgaga tgggcatccg cttctcgatc
     7801 gacgacttcg gcaccggcta ctcctcgctc ggctacctgc agcgcctgcc gctcgacgag
     7861 ctcaagatcg accgcacctt cgtgcgcgac ctgcccgcca acgccagcag cctggcgatc
     7921 gtgcgcacca tcatggcact cgccgacacc ctgcgcatcc gcgtcgtcgc cgagggcgtg
     7981 gaaacctccg cacagcacgc ctgcctgctc gacaacggct gcgacgcctt ccaaggcttc
     8041 ctgttcgccc gccccgaggc ggtggagagc ttccgcgcac ggatgtccgc gacggacgcc
     8101 ttgcacggga gcggcgcgca gccccagggc ggggccgatc gcgaccgatg aacgatccgc
     8161 acccggcaca gcgcgccacc gtgagcctcc ctgccccgac gccagccttg tccccctccg
     8221 cggcagcgat cgagcttttc atcgaggcct gcccgttcgc ggtcatcgag tggggccgcg
     8281 acatgcgcgt cctgcgctgg tcgcccgagg ccgagcgggt tttcggctgg tccgctgcgg
     8341 aggtgctcgg caggcgcccc aacgaatgga gcttcaccca tccggacgac gcggcggcgg
     8401 tcaagcgcgc gatcggccag gccatcaccg cggacggtcc cacaccgccg gtgatcggcc
     8461 gcaacttcac gcgcgacggc cgcctgctgc attgcgaatg gcacaaccgg gcgaaccgcg
     8521 acccgcaagg ccggctcgtc tcgctgctct ctttcgcgaa ggacctcacc cgacagctcg
     8581 aggccgagcg cgcgcgcgac ctcagcgaag cccgctacgc gcacatcttc aacaacagcc
     8641 acgcggtgat gctgatcctc gacccggaga gcggtcgcat cctcgacgcc aaccccgccg
     8701 cggaggagtt ctacggctgg tccagggaaa cgctgcagac gatgcagatc ggggacatca
     8761 acaccctgtc cccgcaggcg ctgctcgtcg aactgaaggc ggcccatgcg gaagagcgca
     8821 agcacttcga gttccgccat cgccgcgccg acggctccgt ccgcgacgtc gaggtccaca
     8881 gcggaccgac cgaggacggc aaccgctcca tggtcttctc gatcgtgcac gacatcaccg
     8941 agcgcaagca ggccgaagcg ttgtcgcgac gctgggagcg cttcttccgc ctgtcgaacc
     9001 tcggcctcgc gatgcacgac gtgtccgaca acacgatcat cgacgtcaat gccacctatg
     9061 cgagccagca cggctactcg atcgaggagc tgcgcggcat gcggatcgac gaactttacc
     9121 ccgaggacga gcgcgagcaa ctccatgctc acctcgccga ggccgatcgc atcggcaacg
     9181 ccagtttcga gaccgttcac ctgcgcaagg acggcagccg gctgccgctc gtgatcggag
     9241 tgaccgccct gctcgacgac cgcggccgcg ccgtcgcgcg cttcgcattc ggactcgaca
     9301 tcagcgcgcg caaggcggcc gaggatgagc tgcgcaagct gtcgcgcgcc gtcgaggaga
     9361 gcccggagag catcgtcatc accaacaccc gggccgagat cgagtacgtc aaccaggcct
     9421 tcatcgacaa gaccggctat tcccgtgccg aggccatcgg ccagaacccg cgcctgctgc
     9481 agtccggacg caccaccccg gccacctatg cggacctgtg gaacacgctc acccacggcc
     9541 gctcctggca gggcgagttc ttcaaccgcc gcaaggacgg cagcgagtac ctcgagcgcg
     9601 tgacgatcac gccgatccac gacgaaagcg ggcacatcac ccactacgtc gcggtcaagc
     9661 aggacatcac cgcgcaacgg cgcatggaag aggaactgct gcgctaccac gaacacctcg
     9721 agggcctcgt cgagagccgg acggccgaac tgcagcacgc actcgatgcg gccaacatcg
     9781 ccagccgcgc aaagagcgag ttcctcacca cgatgagcca cgagatccgc acgccgatga
     9841 acggggtgat cggcctgctc gacgtgctga gccattcgca gctctccacg gaacaggtcg
     9901 agatggtcgg catcatgcgc gaatccgccg agaccctgct gcggctgatc gacgacatcc
     9961 tcgacttttc caggatcgag tcgggcaatc tcgagctcga cgtcggccct gcatcgatcc
    10021 cggacctgat cgcgcgtgtc gtcggcatcc tgcagacggt cgcgaaccgc aagtcggtcc
    10081 ggctgagtac ccgcatcgac cccgacgtgc ccgcggtcgt acgcaccgac gcgctgcgcc
    10141 tgcagcagat cctgtgcaac ctcgtcggca acgcggtgaa gttctcctcc ggcctcgacc
    10201 gccccggccg cgtcgagatt cgcgtcgaga ccgccggcgc aggccggatc cgcttcatgg
    10261 tcaccgacaa cggcatcggc atcgcccccg aggcgatcga gaagatcttc gaccccttct
    10321 cgcaagccga atccagcacc acccgccgct tcggcggcag cggactcgga ctgtcgatct
    10381 gcacgcgcct cgtgcgcatg atgaagggcc ggatggaggt gaacagcctt cccgggcgtg
    10441 gcagccgctt cgtggtcacg ctcccgcttg ccgcgaccaa cggctcggcc acgcgcagcg
    10501 agccagcccg cagcacggcg ctcgctcgcc cctcgcccac gccgccgctt gcgacacccg
    10561 gagccggcga ggccggtcgc cgcatcctcg tcgccgagga caacgacatc aatcgtcgcg
    10621 tcatcgcgcg acagctcgca ctcctcgggc tgcaatgcga caccgccgag gatggcttcg
    10681 aggcactcga gcgctggcgg cagggtcaat acagcctgct gctgaccgat ctccacatgc
    10741 ccgggatgga cggctacgaa ctcacggcga ggatccgcag cgaggaagcg ccgggccggc
    10801 gtacgccgat cgtggcggtc accgcaaacg ccctgcgcgg cgagaaggag cgctgcatcg
    10861 acgccggcat ggacgacttc atcctcaagc cggtacaggt tgcggcgctg caggaggtgc
    10921 tcgcgcgctg gctgcaaccg gacaccgagc agcggactcc cgccggcacc gcgccggcgg
    10981 ccccggcggc gcccccggcg gtgttcgatc cgcaggcctt gccgggactg atcggcaacg
    11041 atgcggcgct gatcgcggag tttctcggcg aataccggct ctccgcatgc gacaccgtgc
    11101 gcagcatccg caaggcgtgc gaggacggcg actggcgccg ggccggcgag ctcgcccacc
    11161 gcctgaagtc ctcctcgcgc tcggtcggcg ccatgcagct cggcgaaatc tgcgccgcgc
    11221 tcgaacaggc cggtcgcgac gacgatggcg accaagtgca gcgccagggc gggctgctcg
    11281 aggcggccct ggcagccacg ctcaccgcga tgcaaggcac gcaggctgca ggagttccgc
    11341 ctgcgcgtgg cgataccccc cggcctgggt agccgctaga atacggatcc caccgacaga
    11401 gccggaaccg atgaacctcg agaccctcaa ggtcttggtc gttaccgacg tcgcggccga
    11461 tgcggcccag atcgcgcgcc tgctcgcgga ccatttcgag cacatcgaga cgtcgacgat
    11521 cgagagccgc ttcatcgccg atttcgagcg cgtccagccc gacgtcgtgg tgctggcctt
    11581 caccagcctc gagcgcgccg agcgctactc gctcggcctc tatcgtcaca gcgaggtggt
    11641 gcactccctg cctcaccgca gcatcgtgct gtgcgacaag gacaacgtcc atcgcgcatt
    11701 cgagctgtgc cgcaaggagt acttcgacga ctacgtcctg tactggccca tggtgttcga
    11761 cacgccacgc ctggccatgt cggtcctcat cgccggccgc tcgttgcagg cccagcggga
    11821 cacgccgaac gcacgcgagg tcgccgcgca tgcacgctcg atcagccagc tggagaccgt
    11881 gctggacgat cgcatcgagg tcggtcgcca gcatgcggac gcgctcgagc gcgcacttca
    11941 ggcagcggaa atcgaagcct ccagcgtgca gccccgccct gcggcgcccg catcgccctc
    12001 cagcgacagc gccaccatcc cggacgcagg catcgacgcc ctgcccgcat tcacggacag
    12061 cttcgagctc gacgtcagcg acgtgccctc gggcctggat cggccgaccc agttcgccgt
    12121 tacaccgcaa gaagacgtca tccaggaagc gaagctgcgt gtggacgagg cgaaaaagaa
    12181 ggtggtgccg ctcaccgaat ggatcggcgg gctcaaggcg gacgtcaagc cgcaactcga
    12241 tgcggctcgc caactcggcg aactcgctgg caggacaccg cccctcatcg tggtggtcga
    12301 cgacgacgcc ttccagtgca agctgctcga acgccttctc ggcaattccg gctaccgttc
    12361 gatcatcgcg cactcgggtg ccgaagccct tgcggtgctc ggccgccagc cccccgatct
    12421 catcctgatg gatgtcgcgc tgcccgactt caacggtgtg gaactcaccc gacgcctgaa
    12481 ggccatcccg cgttttgcgg ccatcccggt ggtgatgatc accggtcaca gcgaacgcca
    12541 ggttctcgaa gccagcctca aggccggtgc ggtggacttc ctggtgaagc cgttcgaccg
    12601 cgagatcctg ctgcagaagc tcgctcgcca cctcgccccg tgaggagggc gtttgagggc
    12661 aaggccggca gccggcagaa ttcgcgtcga cgcgtgaaat atccaccatc gtcgccatat
    12721 ctggaatatt tacaatattc gccaccttca agatgccatc ctctgcccgg agcaccgcag
    12781 caatgatcac cgagatggag atcttcccgt gggacagcaa cttcgccacc ggcatcgcgg
    12841 tcatcgatgc acagcaccag cagctcgtgg cactgctgaa ccgtctcgtc acgcatctcg
    12901 ccttccaatc cgacgccccc acgctcaatt cggtgttcga ccagctgcgc gattacgtcg
    12961 aggtccattt caccaccgag gaggcgctct ggcaggcgca tctgggcgag tacgccggcg
    13021 tcgcgggcca ccatgaatct cacgtccgtt tcatcgacga ggtcctgcgt ctgaaggcgg
    13081 aggaatccac caaacccttc gagacggtga tcgagaacat cgtcaacttt ctgacccgct
    13141 ggctggcact gcacatcctc gaggcggacc gccgcatggc cctggtcgta cacggcgtgc
    13201 agcgcggatt gacgctcgag caggcgagga cgcaagccga cgaaaccatg tcgggttcga
    13261 tgcgcgtcct ggtcgagacc gtgctggtga tgtacgacaa gctcgccaac cgcacgatgg
    13321 cgatgacgcg ggagatcaac cgtcgccgcc aggcggaagc cgaactgcaa aaggcgcacg
    13381 aggcgctcaa gcgcgccaag gagaacgcgg aaagtgtcag cgaggcgaaa tcgtctttcc
    13441 tcgccagcat gagccacgag atccgcacac cgctcgctgc aatcctgggc atgtcgcacc
    13501 tgatgcacca ggaggagttg ccggcgtccc agcgggagcg gctcgacgtg atcgagaacg
    13561 caggcaggca cctgctctcg atcatcaacg gcatcctcga cctgtcgaag atcgaagcgg
    13621 ggaagttcac gctggacgag gccgcactcg acctgcgcgg cgtggtcgag gaggcgctgg
    13681 cgatgctcgc ccaccaggcc gaagccaagg ggctgagcct gcgtgcagag ttcgacctgc
    13741 cgccgggcac cttcctcggc gacgcgacgc gtctgcgcca ggcgctggtc aactacctca
    13801 tcaacgcgct gaaattcacc gagcggggtg gcatcaccgt gcgctgcagc gccagcgcgg
    13861 tcgacacgga cgcggtggac gtccgcttcg tggtcgagga caccgggccg ggcatcgctg
    13921 ccgccgaact gcggcgtctg ttcaagccct tcacccagac cgaggcgacc tcgacgggcg
    13981 agcacggcgg caccgggctc gggctggcga tcaccgcgcg tctcgccgag ctgatgggcg
    14041 ggcaggcggg cgccgagagc gagcctggtc gcggcagtcg attctggttc accacgcgcc
    14101 tgcgcaaggt gaaggccgac gaggtcgtgg cgcccacggg ttcgcccctg gagatccgca
    14161 gcgcgctcgc cgagcggcat ggcggcgcat gcgtgctgct ggccgacgac gacaaggtca
    14221 atcgggaaat cgcccgggtg ctgctgtcgc gcggcgggct ggcgccggaa tgcgcagccg
    14281 acggagaaga ggccgcggcg ctcgccgaac gctgcaagcc ggccctgatc ctcatggaca
    14341 tgcagatgcc ggttgtcgac gggatcgccg cgacgcgcag gatccggacc caggactggg
    14401 ggcgcgaggt gccgatcatc gccctgacgg ccaatgcctt cgccgaggac cgggtccgct
    14461 gcctcgaagc cggcatggac gacttcctga ccaagccgat catgcccgcc gtgctgtact
    14521 cgaagctgct ctactggctg gaccgcagcc gccgcgaacg cgcgcgttcc tgatccttcc
    14581 cgcgcagccg cgcacctaga cccgcgcgct cgggcgctcg gcgatgcggt cgcgccagtc
    14641 ctgcaggttg gccatgccct cggcgccggg cctgaacctc atcagcccgc gcgcgaactc
    14701 gatcgcgcaa aaggccgtga tgtcggcgat ggtgaagcgc tcgccggcga cgaagggctg
    14761 gcggccgagc tcctcgtcga gccagcgcgc cacctcgcgc accttctcgc cctgcgagcg
    14821 gccgaaatcc tcgaactgcg gctgctcgag cggcgccagc ccgggatggg tgtggcggac
    14881 gcagttcatg atgccgccga agaggtagag ctcgacgcgg cggtcggtca tctcgacgaa
    14941 ggcacgctcc tcgaagcttt cgcccatcag gttgggctcg gggtagaggc cttcgaggta
    15001 gctgcagatc gcccgcgtct cgccgaggta gcggccgtcg tcgagttcca gcaccggcac
    15061 cttggcgagc gggctgcgtg cgcggtggtc cgagctgcgg tgctcgccct tgacgaggtc
    15121 gaccgcaacc tcctcgatgc cgcggatgcc cttctcggcg atgaacatct gcacgcggcg
    15181 cggattgggc gcccggggtg atacatagag cttcatgggg tctcctggaa tgtcctgaac
    15241 cggcagggcc gcgggggcac tcagactcca ccgctgcgcg actggccggt ctcgaccatg
    15301 ttgcgcacct tcgccgagaa cgccttggcc tgttcctcgc cgtcaccgtc gcggtcgaag
    15361 ctggacggcg gcatctcgat gccgcgctgc acggcggggc gggcgcggat ctggtcgcgc
    15421 cagcgcgcga ggtcgggcag gtcgtcgatc gccacccccg accagttgtg cgtgcgcacc
    15481 caggcccagt tggcgatgtc ggcgatggaa tagtcgccgg cgaggtactc gttcttcgcc
    15541 agatggccgt cgagcacgcg gaacaggcgc ttgacctcac cctggtagcg gtcgatcgcg
    15601 ggctggatct tctccgggaa atagcggaag aagacgttgg cctggcccat catcgggccg
    15661 atgccgccca tctggaacat cagccactgc agcacgcgcg agcgccccct ggcgtcttgc
    15721 ggcatcagcc ggccggtctt ctcggcgagg tagatgagga tcgcgcccga ctcgaagacc
    15781 gcgaagtcgc cctcgtcgtg gtcgacgatc gccgggatgc gaccgttggg gttgatggcg
    15841 aggaaccagg gctccttctg ctcgttggcg gacaggtcga gcacgcgcag cgagtacgcc
    15901 aggccgagct cctcgagcgc gatcgagacc ttgtggccgt tgggggttgc ggcggtgtac
    15961 agatcgatca tgcgattctc catggctgcg ggccgcggcc cgttcttgca tcatcatccc
    16021 gcccgtgccg gcccggccgc aagcaggccg cacgtgtgag cgtcatcacg cattccgtat
    16081 atgcgctatc gggcggcgcg gctgcgccgg cgcggcgcac gcctctacac tttgtccagg
    16141 acgcctgcgc aggcatcgcc ggatccaccg gccacgagaa tgcaggatgc ccgccgggac
    16201 accgcgttcc ggcaccgacc acaccaccgc aaccgggagt tccctcgatg agctacgaaa
    16261 tccgctcgat cctttacgcc tccgacctga ccccgcgctc gcccgcggtg ttccgccacg
    16321 ccgtgggcct ggcgatgaag ttcaacgcca agctgcacgc ggtcaccgtg acccttccat
    16381 cgagcagcct gccctacgag gagttcctca gcgaggagaa gatggacgag atcaagctcg
    16441 ccggccacaa gaaggccgag gccctgctgc gccatcgcat cgacgtcttc gccgaggcca
    16501 accccgagct cgacgtgaag agcgtgctcg ccagcgtgcg cgcgctcgag ggcgacccct
    16561 cgcgccgcat cctcgacgtc gccaagcatg tgctcgccga cgtgatcgtg atgggctcgc
    16621 gcggccacag caccatcggc gagctgctgc tcggctcggt ggcacacaag gtcaccatga
    16681 aggccgacat cccggtgatg ctggtgccga tcgaccgctg agcgctgcgg tgcttccggc
    16741 gtgggagcga cgcaagtcac gatccggcgc atggaattcg caccgcggcc gatccagcgc
    16801 ggcaatcgcg acttgcgtcg ctcctacgtc cctcnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16921 nnnnnnnnnn nnnnaggcac cacgcggacc gcaagccccc atcccgctca cgcatgcggt
    16981 ggcgcgatcc tgcgcccgca atccgacaac agtcggcgca gccagcggtg gccggtggaa
    17041 tggtggttgc gctcgtgcca gagctggtag aagcgcatcg gtggaaaggc gatcggcgag
    17101 gggatgatcg ccagcggcag caggttggcg tagaactgcg cgaagtgccg ggtggtggtg
    17161 aacaccagat cggtgccctg cagcaggtag ggtgcggcgg tgaaggactg caccgccacg
    17221 cgctcgtcgc ggttgatgcg catcgacgcc agcacgctgt cgatgacccc gcgctggctg
    17281 atcgaatacg gcatcggcac cacgtgcgcg gcgcgcaggt agtcgtcgac gctcatcccc
    17341 ttcttcgcgc acgggtggct ggccgccacc aggcacacga tctcgtcctc gagcagcatc
    17401 gacaggtgca tgcggtgcgg cggctcgggc cagttgccga tcaccacgtc caggtcccct
    17461 tccgcgagcg agcgctcgaa gtcgaagctc ggccccagcg cgtgcaggct caggcgcgcc
    17521 tgcggcgcct cgcggcggaa gcgctcggcc acgctggcga cgaacaccgg cgcgagatag
    17581 tcgggcgagc cgacgcggaa ctcgtggcga gtgctgtgcg gctcgaagga ctccggcgca
    17641 tcggtcatgc ggtcgatctc ggcgagcgcg cccttggcgt gcgacatcag cgcgagcgcg
    17701 cgctcggtcg gcaccatgcc gcccttctcc cgcaccagca gcggatcgcc gaggatctcg
    17761 cgcagacgct tcaaggccgc gctcaccgcg ggctgcgact ggttcaggcg cagcgcggtg
    17821 cgcgacacgc tgcgctcgct gatcaggagc acgaacacgc gcaacaggta ggtgtcgagc
    17881 gggtcgtcac tgcgccgcag gctcatggtg ggtctcctcg tcgttcttgt gtggatccgg
    17941 ggcgggagcg actggccgcc agcagacaga tacgcgagag catatatcac atataaggac
    18001 atatgcgttc cgttaacacg gacccggctt acactgcgtc tcacacgatt cccccagccc
    18061 cgcaccagac caggagacaa gcgcatgggc cgcctgacca cccacgtcct cgacaccgcc
    18121 cacggcaagc ccggaaccgg catcaccgtc accgtgtacc ggctcgacgg cgagcgccgc
    18181 gaagtcgcgc gcagcgtcac caaccacgac ggccgctgcg acaagccgct gctcgaaggc
    18241 gccgcactcg aggccggcaa gtacgagatc gtcttcgccg ccggcgacta cttccgcgcg
    18301 ctgggcctga acctgcccga gccgccgttc gtggacgagg tcgcgctgcg cttcggcatc
    18361 gccgatgtcg ccgagcacta ccacgtgccc ctgctggtgt cgccgtggag ctattccacc
    18421 taccgcggca gctgaacccg gcgcttccac ctccacaccg aaccgaatcg agaacaagag
    18481 aacagaccat ggaagcctac ctgctcgact gggccaacct gctggtccgc tggctgcacc
    18541 tgatcgccgg catcgcctgg atcggcgcgt ccttctactt cgtcatgctg gacaactcgc
    18601 tgaagccgcc gaagaacccg aacgacgcca agcgcggcgt gttcggcgag ctgtgggcgg
    18661 tgcatggcgg cggcttctac cacagccaga aatacctcac cggccccaag ggcgagccgc
    18721 tcacccagga cctgcactgg tcgaagtggg aggcctacac cacctggctg tcgggcatgg
    18781 gcatgatggc cgtcatctac tggatcggcg cctcgagcta cctgatcgac agcagcgtga
    18841 tggcgctctc gcctgccgcc gcggtcggca tctcgatcgc cttcctggtc gccggctggg
    18901 tgttctacga cctgctgtgc cgcaacatga tcggcaagga cggcgtgctc gccgcgatcg
    18961 tgttcgtctt cgtgatggtg gcgaactacg tgctgcacca ggtgttctcg gcccgcggcg
    19021 cctacatcca cgtcggcgcc atgctcggca ccatgatggc ggccaacgtc ttcttccaca
    19081 tcatccccgg gcagaagaag atggtcgagc agatccgccg cggcgagcag gtcgatccga
    19141 tgccgggcac cgtcggcaag cagcgctcgg tgcacaacac ctatttcacg ctgccggtgc
    19201 tgttcatcat gatcagcaac cactacccga tgacctacgc caacgccaag ggctggctgg
    19261 tgctcggcgt gctgatgctg gccggggtgc tgatccgcca gttcttcgtg ctgcgccacc
    19321 gcggccaggt caagtggtgg ctgccggcgg ccggcgtcgc gctgatcgcg ctgctgatgg
    19381 tgctgatggc accgaagcag gtcgatgccg gcggcgagaa ggtgagcttc gccaccgtca
    19441 aggcgatcat ggcccagcgc tgcatcgcct gccacgccga caagccgacc ttcgagggct
    19501 tcgcccaggc gcccaagggc gtgatgctgc agacgcccga gcagatcgcc gcgcacgccg
    19561 tgatgagcgc gcagaccgtg gccagcggct acatgccgct cggcaacctc accgccatca
    19621 ccgaagagga gcgcgagaag atcgccatct ggttcgccca gggcgcgcgc accgcggact
    19681 gaacgacagg aacaggacag gaagagacac catgagcacc cccgccaccg ccccaaccac
    19741 ctgactcacc caattgcggg ggagcattcg cggc
//
LOCUS       DAOQNN010000016        19722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1004_length_19722_cov_10.009407, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000016 DAOQNN010000000
VERSION     DAOQNN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 19722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19722
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1004_length_19722_cov_10.009407"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1957)
                     /gene="parC"
                     /locus_tag="PKV97_02175"
     CDS             complement(<1..1957)
                     /gene="parC"
                     /locus_tag="PKV97_02175"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNB04711.1"
                     /translation="MSTDTPDLFDAPGVPESVAPSTPAGAAPSAASTTRGAAPATASD
                     ADIPPPPAPPADPEPEPEDDGTLALDRYAERAYLAYAMSVVKSRALPQVEDGLKPVQR
                     RILFAMNEMRLSSTSKHVKSARVVGDVIGKYHPHGDSSVYDAMVRVAQDFSLRYPLVD
                     GQGNFGSRDGDSAAAMRYTECRLTPIAELLLSEIDRGTVDFIPNYDGAFEEPQLLPAR
                     LPFVLLNGASGIAVGMATEIPPHNLREVAEATCHLIRHPEATLDDVLAMLPGPDFPGG
                     GQLISTPQTIREAYATGRGSLRLRARWKIEELARGQWRAIVEELPHGVSAAQVLSEIE
                     TLTNPQPRAGKKEVSQEQKNLRQLVLGALDTVRDESSDKAPVRIVLEPKSSRQNRDEF
                     MAVLLAHTSLETSASVNMTMIGRDGRPQQKNLVQILREWIDFRYVTVERRTRHRLDEV
                     DRRIHILEGRMIAFLHIEEVIRVIRESDEPKPALIAAFGLSDVQAEDILEIRLRQLAR
                     LEGFKIEKELAELKDERAGLQHLLDSRAAMTKLILKEIQDDARKYGDDRRTLVEAVAA
                     VAPAEISVPDEPVTVIVSKNGWVRSRQGHGIDPAGIAYKAGDFGFAVLETRTTWPLIV
                     IDGNGRAYTVKVSELPGGRGDGAPITTL"
     gene            complement(1954..2406)
                     /locus_tag="PKV97_02180"
     CDS             complement(1954..2406)
                     /locus_tag="PKV97_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNB04712.1"
                     /translation="MKIALDTNILAYAEGAGEDARCAAARALIEDLAASEVLIPAQAL
                     GELHRILTGKLRRDAETVRGALLDWSDAYEVADSTWPSFQAAIDLACDHRMQIWDGLV
                     LSVAAENRCRLLLSEDLQHGFTWRGVTVINPFEHVAHPLLLAACNSSS"
     gene            complement(2403..2654)
                     /locus_tag="PKV97_02185"
     CDS             complement(2403..2654)
                     /locus_tag="PKV97_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNB04713.1"
                     /translation="MHTISAAEANRQFSSLLRQVSQGETITVLSHGRPVATISPATEA
                     GESGHDRANARARLLARLNREPAAGERSWNRDELYDRDQ"
     gene            complement(2743..4731)
                     /locus_tag="PKV97_02190"
     CDS             complement(2743..4731)
                     /locus_tag="PKV97_02190"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNB04714.1"
                     /translation="MAGTQGTPKQYDESSFRVLKGLEPVRERPGMYTRTDSPAHIIQE
                     VIDNAADEALGGFAKKIHVTLHVDGSVTVADDGRGIPVGLHPEEGVPVVVLAYTRLHA
                     GGKFDKREGNSAYAFSGGLHGVGVSVTNALSTRIEVEVRREGKIHRIDFSDGGETIGP
                     VRVEGDCGRQTGTRVRVWPDGKYFESPRVPMNELERLLRSKAVLLSGVAVRLDIEQAN
                     GPALTKTWSYPDGLAGYLKELAGDVEPVAPIFTAEKYAAKDDPTFAAGEGAAWALAWF
                     ESPVPSESYVNLIPTVNGGTHESGLRAGVFEAMKSFIDHHTLLPRGVKLQQEDVCGRM
                     SFVLSARLLDPQFQGQVKEKLNSREAVKLVSSQLRDPFEIWLNNHVEAGRAIAELSIK
                     QALARQKSAQKVEKKKTSGVAVLPGKLSDCESEDLADNELFLVEGDSAGGSAKMARNK
                     ETQAILPLRGKVQNAWEIDPDRLFANAEIHDIAVALGVDAHTADSPIDLSGLRYGKVV
                     IMSDADVDGAHIQTLLLTLFFRHFPKLIERGHVYVAQPPLYRIDVPAQGKKRPPRRLY
                     ALDEGELAAMRERLEKEGFKPDAIEIGRFKGLGEMNPDQLRETTMDPATRRVLPVKVR
                     SGALQDTLKMFTLLMGKGEASGRRAWMEEKGDSVEADV"
     gene            complement(4796..5287)
                     /locus_tag="PKV97_02195"
     CDS             complement(4796..5287)
                     /locus_tag="PKV97_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNB04715.1"
                     /translation="MNRPAVPPSSADRQRLALLIEAEAIQLGEFVELLAREEALLIAG
                     EVDALVVLCQDKNERYRRLQRLSDDRSLLLGRFGQAVSDTVIRDWLAGLPRVLARWDE
                     VLGLARTTRERNALNGKLITERMAHNQAALSLLLSAADQPPLYDAEGHTRAIGGGRML
                     GSA"
     gene            complement(5302..5601)
                     /gene="flgM"
                     /locus_tag="PKV97_02200"
     CDS             complement(5302..5601)
                     /gene="flgM"
                     /locus_tag="PKV97_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNB04716.1"
                     /translation="MKIESSGKAIAPSTAAEARPRSPASPGATVSAGSEKVQLSSLSS
                     SLAKAEASIAQTPVVDAQRVEEIKQAIANGEFRIDAGRIADGLIDSVREMLAGQR"
     gene            complement(5734..6462)
                     /gene="flgA"
                     /locus_tag="PKV97_02205"
     CDS             complement(5734..6462)
                     /gene="flgA"
                     /locus_tag="PKV97_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNB04717.1"
                     /translation="MTTPLHRRLAAGRLRGPAAALCAALVLLPSPAAIAQHAEGAVEA
                     RVRQFLQRQVAGLPGEVSIELAPLDPNSQLPACAALEPFLPAGTRAWGRFSVGVRCDS
                     PVTWTAYLQARVAVVADYLIAARPLRAGQVLGPADLGQRRGDLTALPDNLLIDPTQAS
                     GHHTRIAVAAGSPLRGDMLRVPHAVRQGQTVSVLGVGAGFRVASEGRAMNNAAPGEKV
                     RVRLADGQVVTGTAQAGGAVALEF"
     gene            6659..7063
                     /gene="flgB"
                     /locus_tag="PKV97_02210"
     CDS             6659..7063
                     /gene="flgB"
                     /locus_tag="PKV97_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNB04718.1"
                     /translation="MKTLFDRQIQFHQTALNLQAHRQQLIASNIANADTPHYKARDVD
                     FRAALQGALNASREGVQMATTQAGHIGSAPAPALESLTGYRSELQSAVDGNTVNMDVE
                     RAAFAENALHYEASVTFINGMLRSMNTAITGQ"
     gene            7075..7479
                     /gene="flgC"
                     /locus_tag="PKV97_02215"
     CDS             7075..7479
                     /gene="flgC"
                     /locus_tag="PKV97_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNB04719.1"
                     /translation="MSMLNVFQIAGSALNAQSLRLNATASNLANADSVTSEDGQPYRA
                     KQVVFAARPVAGPAAVGVQVTQVVESAAPLRLVYEPNNPAANPDGYVEMPNVNVVEEM
                     VNMISASRSYQNNAEVMSTARTLLQRTLQIGQ"
     gene            7530..8204
                     /locus_tag="PKV97_02220"
     CDS             7530..8204
                     /locus_tag="PKV97_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HNB04720.1"
                     /translation="MSSVTNNRDIAASVLAKINTREESKASASGDMNSRFLTLLTTQL
                     KNQDPLNPMENAEVTSQLAQMSTVDGIERLNKMFQSFVDGQSAYDSMQAAALVGRGVL
                     VEGKKLTLTEAGAVGGFELDAAADSVVLSIKDAAGLEIKKIELADVKAGSQNFIWDGT
                     ASDGSRAVDGAYTVELSALAEGKKVTGRTLEFGPVTSVIRGASGTDFQVGSLGIFKYD
                     DIKQIL"
     gene            8220..9572
                     /gene="flgE"
                     /locus_tag="PKV97_02225"
     CDS             8220..9572
                     /gene="flgE"
                     /locus_tag="PKV97_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNB04721.1"
                     /translation="MSFQQGLSGLSVSSKALDIISNNVANTNTIGFKTGSAIFSDVFA
                     ASLTGSISGKQVGVGATLGAVRQTFTQGNLTTTNNPLDLAINGDGFFVVSRENGPDVY
                     TRNGQFELDKEGFIRTPTGERLKGFQSPVTPGALPTPIGGLKDVQVPIVGSQPMPTSR
                     VEIGGNLDANDLSAAQRYPQLFGSTFTFPLPAGENWQDARTYNFSTSIKAYDSLGKSH
                     ELTYYFAKVTPTPNDPVNTNENTWKVFTSVDGGYPLGVGTDGSIEDPSVIWSLTFNEK
                     GTVNTPIVGPTAANPLAIPASYTPGADRIRFHVDFSDMRQIGGSYLITELTQDGYTGG
                     ELAGVSVGRDGIVTGRYTNGETRELAQLALQTFRNPNALLSIGNNFWEATTESGLTAP
                     SKAGEGVAGVVSAGMIEDANVELTNELVQMIVQQRNYQANAQSIKAQDQVLQTLVNLR
                     "
     gene            9605..10345
                     /gene="flgF"
                     /locus_tag="PKV97_02230"
     CDS             9605..10345
                     /gene="flgF"
                     /locus_tag="PKV97_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HNB04722.1"
                     /translation="MDRLIYTAMTGAKGTMDQQAAVAHNLANVDATGFRAELHKLRAV
                     EVQTEALRTRAFTVDASVANDFTEGPLQFTGRPFDVALAGKGWLAVQMPDGSEAYTRN
                     GSLAVSANGVLQTRDGRPVLGDGGPVTIPPDNEITIGADGSVSALQPDQPGVVNVVAQ
                     LKLVNPPEAQLVRGDDGLFRLQGGAPAPVDPNVRVAGGYLEGSNVNVVDQMVQMISLA
                     RQFEMQTRMLSTAEQNDRSAARVIGGQG"
     gene            10594..11376
                     /gene="flgG"
                     /locus_tag="PKV97_02235"
     CDS             10594..11376
                     /gene="flgG"
                     /locus_tag="PKV97_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNB04723.1"
                     /translation="MIRSLWTARTGLDAQQTSLDVISNNLANVSTNGFKRQRAIFEDL
                     LYQTIRQPGAQSTQQTQISSGLQIGTGVKPVATERIFTQGTLQQTGGSLDVAVQGNGF
                     FQVAMPDGTTAYTRDGAFQLDSQGNVVTASGYTLADNINIPADALSITIGKDGTVSVL
                     QAGQTAPVEIGAIQLATFINSGGLQAAGENLFYETASSGVATPTQPGTNGAGVLNQGY
                     VEVSNVNVAEELVSMIVTQRAYELNSRAISTSDQMLGRLTQL"
     gene            11385..12062
                     /locus_tag="PKV97_02240"
     CDS             11385..12062
                     /locus_tag="PKV97_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNB04724.1"
                     /translation="MKLARHSIAVGLLALLSGCAAIQTTPPSAVHQPMSVRPEAMATA
                     LPANGAIYQTVQARPLFEDRRARRIGDTITINLVERNTAQKSANANATRNGNMSAGIG
                     PINRLPLSGLNGLELEADAESDFNGKGAAAANNVFNGTITVTVIDVYPNGNLLVSGEK
                     MVAINQGNEFIRFSGVINPNTVTTANTVQSTQVADARIEYRGSGFIDESNTMGWLQRF
                     FVAIMPF"
     gene            12073..>12464
                     /locus_tag="PKV97_02245"
     CDS             12073..>12464
                     /locus_tag="PKV97_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNB04725.1"
                     /translation="MSCLASLLLRAAVVSLAVLILHPAQAYAAAERIKDLASVAGVRD
                     NQLVGYGLVVGLDGSGDQTTQTPFTVQSIVNMLGNLGVSLPPGTNLQLKNVAAVMVTA
                     NLPAFARPGQAIDVTVSSIGNAKSLRGGT"
     assembly_gap    12465..12564
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12565..13070
                     /locus_tag="PKV97_02250"
     CDS             <12565..13070
                     /locus_tag="PKV97_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNB04726.1"
                     /translation="ARRVVDAINLVYPGAASALDGRSIQVRAPTDSSARVSFLGHIEN
                     LEVTPVQQAAKVIVNSRTGSVVMNQRVMLQNCAVAHGSLTVTVNNEAQVSQPPPLSGG
                     QTVVTQSSQVDIAQEGGTLFNVRGGANLADVVKALNTLGAKPMDLVSILQAMKAAGAL
                     RAELEVI"
     gene            13081..14139
                     /gene="flgJ"
                     /locus_tag="PKV97_02255"
     CDS             13081..14139
                     /gene="flgJ"
                     /locus_tag="PKV97_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HNB04727.1"
                     /translation="MNKGFQLNALDPNSLGDLKRLARSEGGSDAALRAAAKQFEGLFL
                     QMVMKSMRATTGQNGMMDSEQTRSWQGMLDQQMALEMSQSGGTGLSEMLYRQLGGKAA
                     VRPDAAGEAGKTGFDLADVRRRPAIPAVLARDAESAAAPRLAALPAEAGVPALGAAAT
                     AGEFSALSVYERAALQTRLDAAVRSARDAGGEVSKSAQDFVTEVWPHAQEASRRTGIP
                     AEFMVAQAALETGWGQKQLRHEDGSPSHNLFNIKAGSAWSGRTVAREVTEYADGQAYT
                     EAAHFRSYGSYAESFRDYAQLMTRSPRYAGVLGQTDAAAFARGLQDAGYATDPMYADK
                     LTRIIGGNTLRSALAMAG"
     gene            14221..16203
                     /gene="flgK"
                     /locus_tag="PKV97_02260"
     CDS             14221..16203
                     /gene="flgK"
                     /locus_tag="PKV97_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNB04728.1"
                     /translation="MASLLNIGLTGLSASQAYLNTTSHNIANAATPGFHRQTVTQEAR
                     DPQYSGGAFFGTGTGVTGVARSYSQLLEGQVLSADSRRAEYAAYSQFISQLDNVLADA
                     DTGLTPALNEFFGALQNVTSNPTNVAARQALLSSGDTLSARFRTLDGRISEIAEGVET
                     EMRSTLESINGYAAAIAELNERMVISRTGGAGSAANDLLDQRDQAIAELNRLVKVSTV
                     EQRDGSTSVFIGSGQSLVMGNRAKQLELGDQLDENGRSMITVRANSGVVEPLAESLVG
                     GGTLGGLLAARREGTASAQRSLGLLATTLATEFNSQHALGLDLEGRLGGDFFRIGGVR
                     VDPSDSGLSVRIDPGQVSSLTGSDYSLRYDGAAFQLTDLATGNAVPAAQMQLVTEADG
                     STTIRAQGLVFTGARSTLTEPAFVYPTRYTAGAIEVANRDPRAVAAAAPVLASPTRGD
                     GSSLRIEQSGLLSRGSAAAGTELGLPALKYTFNAVDKRFELDAAAPGAAGWPATLAYD
                     PATSAAGESFTLEGPGGVRLELKISGTPAANDVIALADNKSGVADNRNAALLGALQTD
                     KLLFGSGGQATATLNNAYAQLVAKVGSKTREVQAGERTQTSLLDQAKASRDAVSGVNL
                     DEEAANLVRFQQSYQAAAKVMGVAQRLFDEILAIAR"
     gene            16218..17126
                     /gene="flgL"
                     /locus_tag="PKV97_02265"
     CDS             16218..17126
                     /gene="flgL"
                     /locus_tag="PKV97_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNB04729.1"
                     /translation="MRVSTNMIYDQGVIALQKQSAALLHTGQQIATGRKILTPSDDPV
                     ASARALDVSQSKSVNQQFITNQGYADDALKLVDGQLDGAGEIIQYARERVLAAGNPAL
                     SPSDRQSIASDLRSQFDAMLAIANTRDANGDYLFAGYRSNTPPFEKTVTAGTLGSDYK
                     GDQGSRTMQVSSSRFMPISFGGESVFGSEGDGVFKVLGEFISALEGGGDVSGAVSTAL
                     GGLDDSLDRTLTVRAQIGSQQVEVEQLGFIGSDLDLQYQTTLSNLQDLDYNEAISRLT
                     QQRTFLEAAQQSFLKTTGLSLFNYLS"
     gene            17132..17608
                     /locus_tag="PKV97_02270"
     CDS             17132..17608
                     /locus_tag="PKV97_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04730.1"
                     /translation="MRRALLVLAACSVLGCASEREGTGLLDRAAAAPGPIAGAVGAVW
                     MASELSYLEPEYLAGMLVAYGIYDPLAPTWSIEVSRSGEDRVRMDLHMKRMTTGGDGE
                     ARRVFLRNAREIVRAGGFAGFDELSWEEGIESTRPFARRVASGEIRLVRSRTFPEF"
     gene            complement(17620..18417)
                     /gene="fliR"
                     /locus_tag="PKV97_02275"
     CDS             complement(17620..18417)
                     /gene="fliR"
                     /locus_tag="PKV97_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNB04731.1"
                     /translation="MLSVTAAQLDAWLAALMFPLGRLLGLFASAPVFSNRGVSVRIRL
                     AIGLGVAMAVLPVLPPMPQVPPGGGLGLAIFAQQILIGIAIGFVMRIVFAAVDMAGAL
                     IGMQMGLSFAVFFDPDAGGQTAVLSDFLNLLATLIFLAIDGHLLMIDLLVRSFEWLPV
                     ATDAVDAGGWAFLARSGITVFSAGLLLSLPVIAVLLVANIALGVLTRAAPQLNLFAVG
                     FPITLTAGFIGVLLIMSNFAPVLQSLFERSYDTIGLLLDALAPRPTP"
     gene            complement(18452..18721)
                     /gene="fliQ"
                     /locus_tag="PKV97_02280"
     CDS             complement(18452..18721)
                     /gene="fliQ"
                     /locus_tag="PKV97_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNB04732.1"
                     /translation="MTPTTVIELGRQAIEVTLMVSAPLFLAALVTGLIISIFQAATQI
                     NEMTLSFVPKLVAIFVTLVLAGPWMITLLTDFIRRLFESIPAMIG"
     gene            complement(18747..19445)
                     /gene="fliP"
                     /locus_tag="PKV97_02285"
     CDS             complement(18747..19445)
                     /gene="fliP"
                     /locus_tag="PKV97_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766452.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNB04733.1"
                     /translation="MWPALDTQALPALTTSPAAGGGTTYSLTIQTLMLMTLLSFIPAL
                     VLMTTCFTRIVIVFSLLRQAMGTQTAPPNQVLLGLALFLTFFVMAPVGERVYTEAYLP
                     MSEGRIQFDEALERASVPVKGFMLKQVREPDLALFAGLAKLPPVDKAEDLPMRVVIPA
                     FVTSELKTAFQIGFIVFIPFIIIDMVVASVLMSMGMMMMSPVIVSLPFKIMLFVLVDG
                     WTLLIGSLVQSFAV"
     gene            complement(19482..19661)
                     /locus_tag="PKV97_02290"
                     /pseudo
     CDS             complement(19482..19661)
                     /locus_tag="PKV97_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3227 a         6734 c         6710 g         2951 t
ORIGIN      
        1 ccagcgtggt gatcggcgcg ccgtcgccgc gcccgccggg cagctcggac acctttaccg
       61 tgtaggcgcg gccgttgccg tcgatgacga tcagcggcca ggtggtgcgt gtctcgagca
      121 ccgcgaagcc gaagtcgcca gccttgtagg cgatgccggc agggtcgatg ccgtggccct
      181 ggcgcgagcg cacccagccg tttttcgaca cgatcacggt gaccggctcg tccggcacgc
      241 tgatctcggc gggtgcgacc gccgccacgg cctccacgag cgtgcggcgg tcatcgccgt
      301 acttcctggc gtcgtcctgg atctccttca ggatcagctt cgtcatcgcc gcgcggctgt
      361 cgaggaggtg ctgcagtccg gcgcgctcgt ccttgagctc ggcgagctcc ttctcgatct
      421 tgaaaccttc caggcgcgca agctggcgca ggcggatctc gaggatgtcc tcggcctgga
      481 cgtcggacag cccgaaggcc gcgatcagcg ccggcttggg ctcgtccgac tcgcggatca
      541 cgcggatcac ttcctcgatg tgcaggaagg cgatcatgcg gccttcgagg atgtggatgc
      601 gccgatcgac ctcgtccagc cggtggcggg tgcggcgctc gacggtcacg tagcggaagt
      661 cgatccattc gcgcaggatc tgcaccaggt tcttctgctg cggccgcccg tcgcggccga
      721 tcatggtcat gttgaccgag gccgaggtct ccaggctggt gtgggcgagc agcaccgcca
      781 tgaactcgtc gcggttctgc cggctcgact tgggctccag cacgatgcgc accggcgcct
      841 tgtcgctgga ctcgtcacgt accgtgtcga gcgcgccgag cacgagctgc ctgaggttct
      901 tctgctcttg cgagacctcc ttcttgccgg cgcgcggctg cgggttggtc agggtctcga
      961 tctcggacag cacctgtgcg gcggacacgc cgtgcggcag ctcctcgacg atggcgcgcc
     1021 actggccgcg ggcgagctcc tcgatcttcc agcgcgcgcg caggcgcagg ctgccgcggc
     1081 cggtcgcgta ggcctcgcgg atggtctggg gcgtggagat gagctggcca ccaccgggga
     1141 agtcggggcc gggcagcatg gccagcacgt cgtcgagcgt ggcctcgggg tggcggatca
     1201 ggtggcaggt ggcctcggcc acctcgcgca ggttgtgcgg cgggatctcg gtcgccatgc
     1261 ccaccgcgat gcccgaggcg ccgttcaaca gcacgaaggg caggcgcgcg ggtagcagtt
     1321 gcggctcctc gaaggcgccg tcgtagttgg ggatgaagtc caccgtgccg cggtcgatct
     1381 cggacagcag cagctcggcg atcggggtca ggcggcattc ggtgtagcgc atcgccgccg
     1441 ccgagtcgcc gtcgcgcgag ccgaagttgc cctggccgtc gaccagcgga tagcgcagcg
     1501 agaagtcctg cgccacgcgg accatggcgt cgtacacgct ggagtcgccg tgcgggtggt
     1561 acttgccgat cacatcgccg accacgcgcg ccgacttcac gtgcttggag gtggacgaca
     1621 gccgcatctc gttcatcgcg aacaggatgc ggcgctgcac gggcttcagg ccgtcctcga
     1681 cctgcggcag cgcgcgcgac ttcaccacgc tcatcgcgta ggcgaggtaa gcgcgctcgg
     1741 cgtagcggtc gagcgccagc gtgccgtcgt cctcgggctc gggttccggg tcggcgggcg
     1801 gggcaggggg cggggggatg tctgcgtcgg aggcggtcgc aggcgctgcg ccccgagttg
     1861 tcgaggcagc actcggggct gcgccggccg gggtcgaggg ggcgacggat tcgggcacgc
     1921 caggcgcgtc gaagaggtcg ggagtgtctg tgctcatgag ctcgaattgc atgctgcgag
     1981 cagtagcggg tgcgcgacat gctcgaaagg attgataacg gtgacgccgc gccaggtgaa
     2041 gccgtgctgc aggtcttcgc tcagcagcag gcggcaacgg ttttccgcag ccaccgaaag
     2101 gaccaggcca tcccagatct gcatgcggtg atcgcaggcc aggtcgatgg cggcctggaa
     2161 gctcggccag gtcgaatcgg ccacctcgta ggcatcggac cagtcgagca gggcgccgcg
     2221 cactgtctcg gcgtcgcgcc tgagcttgcc ggtgaggatc cggtgcaact cacccaaggc
     2281 ctgcgccggg atcagcacct cgctcgccgc gagatcctcg atcagcgcac gagccgctgc
     2341 acaacgcgcg tcctctccgg cgccctctgc gtaggcgagg atgttggtgt ccagcgcgat
     2401 cttcattggt cgcggtcgta gagttcgtcg cggttccagc ttcgttcgcc tgcggcaggt
     2461 tcccgattga ggcgggcgag cagacgggcg cgtgcgttgg cacggtcgtg accggattcg
     2521 cccgcttctg tggcggggct gatcgtggcg acgggacggc cgtgggacag gaccgtgatg
     2581 gtctcgccct ggctgacctg gcggagcagg ctggagaact gtcgattcgc ttcggcggcg
     2641 gaaatggtgt gcatggcagg tccgtagctt gcatcggtgt agtgaatatc actaccttat
     2701 tccaggtgat ggcgtcgggc aattcgattt gccttggggg gctcagacat ccgcctcgac
     2761 gctgtccccc ttttcttcca tccacgcccg ccgccccgat gcttcgccct tgcccatcag
     2821 cagggtgaac atcttcagcg tgtcctgcag cgcgccgctg cgcaccttca ccggcagcac
     2881 ccgacgggtg gccgggtcca tggtggtctc gcgcagctgg tcggggttca tctcgcccag
     2941 gcccttgaag cggccgatct cgatcgcgtc gggcttgaag ccttccttct ccaggcgctc
     3001 gcgcatcgcg gcgagctcgc cctcgtcgag cgcgtagagg cggcgcggcg ggcgcttctt
     3061 gccttgtgcc gggacatcga tgcggtacag gggcggctgc gccacataca cgtggccgcg
     3121 ctcgatgagc ttggggaagt ggcggaagaa cagcgtcagc agcagggtct ggatgtgggc
     3181 gccgtcgacg tcggcgtcgg acatgatgac gaccttgccg tagcgcagcc cggagaggtc
     3241 gatcgggctg tcggcggtgt gcgcgtccac gccgagggcc accgcgatgt cgtggatctc
     3301 ggcgttggcg aagaggcgat ccgggtcgat ctcccaggcg ttctgcacct tgccgcgcaa
     3361 gggcaggatg gcctgggtct ccttgttgcg cgccatcttg gccgagccgc cggcggagtc
     3421 gccctcgacc aggaagagct cgttgtcggc gaggtcctcg gactcgcagt ccgacagctt
     3481 gccgggcagc accgccacgc ccgaggtctt cttcttctcg accttctgcg cgctcttctg
     3541 gcgcgcaagc gcctgcttga tggaaagctc ggcgatcgcg cggccggcct cgacgtggtt
     3601 gttgagccag atctcgaagg ggtcgcgcag ctggctggaa acgagcttga ccgcctcgcg
     3661 cgagttgagc ttttccttca cctggccctg gaactgcggg tcgagcaggc gcgccgagag
     3721 cacgaagctc atgcgcccgc acacgtcctc ctgctgcagc ttcacgccgc gcggcagcag
     3781 ggtgtggtgg tcgatgaagg acttcatcgc ctcgaacacg ccggcgcgca ggccggattc
     3841 gtgggtgccg ccgttcacgg tcgggatcag gttgacgtag gactcgctcg gcaccgggga
     3901 ttcgaaccat gccagcgccc acgccgcgcc ttcaccggcg gcgaaggtcg gatcgtcctt
     3961 ggcggcgtat ttctcggcgg tgaagatcgg cgccaccggc tcgacgtcgc ccgcgagctc
     4021 tttcagataa ccggcgaggc cgtcggggta ggaccacgtc ttggtcagcg ccgggccgtt
     4081 cgcctgctcg atgtcgagcc gcaccgcgac ccccgacagc agcaccgctt tcgagcgcag
     4141 caggcgctcg agctcgttca tcggcacgcg cggagattcg aagtacttgc cgtccggcca
     4201 cacccgcacc cgggtgccgg tctggcgtcc gcagtcgccc tcgacgcgca ccgggccgat
     4261 cgtctcgccg ccgtcggaga agtcgatgcg gtggatcttg ccctcgcgcc tgacctcgac
     4321 ctcgatccgg gtcgacagcg cgttggtcac cgacacgccg acgccgtgca ggccgcccga
     4381 gaaggcatag gccgagttgc cctcgcgctt gtcgaacttg ccgccggcgt gcagccgggt
     4441 gtaggccagc accaccaccg ggacgccttc ctcggggtgc aggccgaccg ggatgccgcg
     4501 gccatcgtcg gccacggtga ccgagccgtc gacatggagc gtgacgtgga tctttttcgc
     4561 gaagccgccg agcgcttcgt cggcggcgtt gtcgatcacc tcctggatga tgtgcgccgg
     4621 gctgtcggtg cgggtgtaca tgccggggcg ttcgcgcacc ggctcgagcc ctttcaggac
     4681 gcggaaggag gattcgtcgt actgctttgg agtgccttgc gtgccggcca tgctgactgc
     4741 ccgttcgaac ttgcgaaggg cgcaaggata gcagagcgaa gggctgtatg tatgttcagg
     4801 cgctgccgag catgcgcccg ccgccaatgg cgcgggtatg gccttcggcg tcgtagagcg
     4861 gcggctggtc ggcggccgag agcagcaggc tgagcgcggc ctgattgtgc gccatgcgct
     4921 cggtgatgag cttgccgttg agggcgttgc gctcgcgcgt ggtgcgggcc aagccgagga
     4981 cctcgtccca gcgcgccagc acgcgcggca agccggcgag ccagtcgcgg atcacggtgt
     5041 cggagaccgc ctggccgaag cggccgagca gcaggctgcg gtcgtcggag agccgttgca
     5101 ggcggcggta gcgttcgttc ttgtcctggc agagcaccac cagtgcatcg acctccccgg
     5161 cgatcagcag cgcctcctcg cgcgcgagca gctcgacgaa ctctccgagc tggatcgctt
     5221 ccgcttcgat cagcagcgcg aggcgctggc ggtcggcgga ggagggcggg acggcaggac
     5281 ggttcatgcg cggtgaagcg ttcagcgctg gcctgcgagc atctcgcgca cgctgtcgat
     5341 cagcccgtcg gcgatgcgtc cggcgtcgat cctgaattcg ccgttggcga tcgcctgctt
     5401 gatctcctcg acgcgctgcg cgtcgacgac cggggtctgg gcgatgctcg cctcggcctt
     5461 ggcgaggctg gacgacagcg aggagagctg gaccttttcg ctgccggcgg agaccgtggc
     5521 gccggggctc gcggggctgc gtgggcgggc ctccgctgcg gtggaggggg caatcgcctt
     5581 gcccgagctt tcgattttca tggcgcagtt cccatacaga gtgactctga cgttctcttc
     5641 ggcgcgatgg cgtggaactt gagcgattaa agcatgaaat ctttcacgct cggccatccg
     5701 ctcgcggccg tctccgttcg gcacgcagcc gcctcagaac tccagcgcca ctgcgccgcc
     5761 cgcctgtgcg gtcccggtga cgacctggcc gtcggccagg cgcaccctca ccttctcacc
     5821 cggggcggcg ttgttcatcg cacggccttc gctcgccacc cggaagcccg cgcccacgcc
     5881 gagcacgctc acggtctggc cctggcgcac cgcgtggggt acgcgcagca tgtcgccgcg
     5941 caaggggctg ccggcggcga ccgcgatgcg ggtgtggtgg ccgctggcct gggtggggtc
     6001 gatgaggaga ttgtcgggga gcgcggtgag gtcgcccctg cgctgcccga ggtcggcggg
     6061 gccgagcacc tggccggcgc gcagcgggcg ggcggcgatc aggtagtcgg cgaccacggc
     6121 gacgcgcgcc tgcaggtagg cggtccacgt caccggtgag tcgcagcgca caccgacgct
     6181 gaagcgtccc cacgcgcgcg tgcccgcggg caggaagggt tcgagcgcgg cgcaggcggg
     6241 caactggctg ttgggatcga gcggcgcgag ctcgatcgac acctcgccgg gcaggccggc
     6301 gacctggcgc tgcaggaact ggcgcacgcg ggcctcgacc gccccctcgg cgtgctgggc
     6361 gatcgcggcg ggggagggaa gcagcacgag ggccgcgcac agcgctgcgg ccgggccgcg
     6421 cagtcgccct gcggcgagcc tgcggtggag gggagtcgtc atggcgggat tgcaccatga
     6481 cccccgccgg cctggcaacc ggcagcgtcc gccggcaatc cttgccggcg ggtggcggcg
     6541 aaattggccg ggctttgccc cccttatcgg cgccagggtc ttgccggccc gcgcctacca
     6601 tcgctggcat ggaaactgcg tccggaacgt ccgggcgcac cgtcccggga gcacagcgat
     6661 gaagaccctt ttcgaccggc agatccagtt ccaccagacc gcgctgaacc tgcaggcgca
     6721 ccgccagcag ctgatcgcct cgaacatcgc caacgccgac acgccgcact acaaggcgcg
     6781 cgacgtggat ttccgcgccg cgctgcaggg cgcgctcaat gcctcgcggg agggtgtgca
     6841 gatggcgacc acgcaggcgg gccacatcgg cagcgcaccc gcgcctgcgc tcgaaagcct
     6901 gaccggctac cgcagcgagc tgcagtccgc ggtggacggc aacacggtga acatggacgt
     6961 cgagcgcgcc gccttcgccg agaacgcctt gcactacgag gccagcgtga ccttcatcaa
     7021 cggcatgctg cgctcgatga acaccgcgat caccggccag taaggagagc cgccatgagc
     7081 atgctgaacg tcttccagat cgccggctcg gcgctcaatg cgcagtcgct gcgcctcaat
     7141 gccaccgcct ccaaccttgc caacgccgac agcgtcacct ccgaggacgg ccagccctac
     7201 cgcgccaagc aggtggtgtt cgccgcgcgc ccggtcgccg gcccggcggc ggtcggcgtg
     7261 caggtgaccc aggtggtcga gagcgccgcg cccctgcggc tggtctacga gcccaacaac
     7321 cctgccgcca accccgacgg ctacgtcgag atgcccaacg tcaacgtggt cgaggaaatg
     7381 gtgaacatga tctccgcctc gcgttcctac cagaacaacg ccgaggtgat gagcacggca
     7441 cgaaccttgc ttcagcgtac cttgcagatc ggccagtaag caccccgcag gcgccgtgac
     7501 gcggaaaacg ccgacagcag gagagaggaa tgagcagcgt aacgaacaac cgggacatcg
     7561 cggcgagcgt gctcgccaag atcaacacgc gcgaagagtc gaaggcttcg gcctccggcg
     7621 acatgaactc gcgcttcctc accctgctca ccacccagct gaagaatcag gatccgctca
     7681 acccgatgga gaatgcggag gtcacctcgc agctcgcaca gatgagcacg gtcgacggca
     7741 tcgagcgcct gaacaagatg ttccagagct tcgtcgatgg ccagagcgcc tacgactcga
     7801 tgcaggctgc cgcgctcgtc gggcgcggcg tgctggtgga ggggaagaag ctgaccctca
     7861 ccgaggccgg cgcggtaggc ggtttcgagc tcgatgccgc ggccgacagc gtggtgctgt
     7921 cgatcaagga cgctgccggc ctcgagatca agaagatcga gctcgcggac gtcaaggcgg
     7981 gcagccagaa cttcatctgg gacggtaccg ccagcgacgg ttcgcgtgcc gtcgacggcg
     8041 cctacacggt cgagctgagc gcgctcgccg agggcaagaa ggtcacgggt cggacgctcg
     8101 agttcggccc ggtcaccagc gtgatccgcg gcgcctcggg taccgacttc caggtcggct
     8161 cgctcggcat cttcaagtac gacgacatca agcagattct ctgatccgga gccccgatca
     8221 tgtctttcca gcaaggtttg agcggcctga gcgtttcctc caaggccctg gacatcatct
     8281 ccaacaacgt cgccaacacc aacacgatcg gcttcaagac cggctccgcg atcttctcgg
     8341 acgtctttgc ggcctcgctg accggctcga tctcgggcaa gcaggtgggt gtgggcgcca
     8401 cgctcggtgc ggtgcggcag accttcaccc agggcaacct caccaccacc aacaacccgc
     8461 tcgacctggc gatcaacggc gacggcttct tcgtggtcag ccgcgagaac ggcccggacg
     8521 tgtatacccg caacggccag ttcgagctcg acaaggaggg tttcatccgc acccccaccg
     8581 gcgaaaggct caagggcttc cagagtccgg tcaccccggg cgccctgccc acgccgatcg
     8641 gcggcctcaa ggacgtccag gtgccgatcg tcggttccca gcccatgccg accagccggg
     8701 tggagatcgg cggaaacctc gatgcgaacg acctgagcgc agcccagcgc tacccacagc
     8761 tgttcggctc gaccttcacg tttccgctcc ctgcgggcga gaactggcag gacgcgcgca
     8821 cctacaattt ctcgacctcg atcaaggcgt acgacagcct cgggaagtcg cacgagctca
     8881 cctactactt cgccaaggtg accccgaccc cgaacgatcc ggtcaacacg aacgagaaca
     8941 cctggaaggt cttcaccagc gtggacggcg gctatccctt gggggtcggg accgatggct
     9001 cgatcgagga cccgagcgtg atctggagcc tgaccttcaa cgagaagggt acggtgaaca
     9061 cgcccatcgt cgggccgacg gctgcgaatc cgctggcgat cccggcttcc tacacgccgg
     9121 gtgcggaccg catccgtttc cacgtcgatt tcagcgacat gcgccagatc ggtggctcct
     9181 acctgatcac cgagctcacc caggacggct acaccggcgg cgagctcgcc ggagtcagcg
     9241 tggggcggga cggcatcgtg accggtcgct acaccaatgg cgagacgcgc gaactcgccc
     9301 agctcgcgct gcagaccttc cgcaatccga acgcgctgct gtcgatcggc aacaacttct
     9361 gggaagcgac caccgaatcc ggcctcactg cgccgtccaa ggccggcgaa ggggtcgcgg
     9421 gcgtggtctc ggcgggcatg atcgaggacg ccaacgtgga gctcaccaac gagctggtgc
     9481 agatgatcgt gcagcagcgc aactaccagg ccaacgcgca atccatcaag gcgcaggacc
     9541 aggtcctgca gaccctggtg aacctgcgct gatcctgaaa cccgcctgac acgagaggcc
     9601 agcgatggac cgcctgatct acaccgcgat gaccggcgcc aagggcacca tggaccagca
     9661 ggcggcggtc gcgcacaacc tcgccaacgt cgacgccacg ggtttccgcg cagagttgca
     9721 caagctgcgc gcggtcgagg tgcagaccga ggcgctgcgc acgcgcgcct tcacggtcga
     9781 cgccagcgtc gccaacgact tcaccgaggg gccgctgcag ttcaccggcc ggcccttcga
     9841 cgtcgcgctc gccggcaaag gctggctggc ggtgcagatg ccggacggca gcgaggccta
     9901 cacccgcaac ggcagcctcg cggtgagcgc caacggcgta ctgcagacgc gcgacggcag
     9961 gccggtgctg ggcgacggcg ggccggtcac gatcccgccc gacaacgaga tcaccatcgg
    10021 tgccgacggt tcggtctcgg ccttgcagcc cgatcagccg ggtgtggtca acgtggtcgc
    10081 gcagctcaag ctggtgaatc cgcccgaggc gcagctggtg cgtggcgacg acggcctctt
    10141 ccgcctgcag ggcggggcgc ccgcgccggt cgatcccaat gtgcgcgtcg ccgggggcta
    10201 tctcgagggc agcaacgtca acgtggtcga ccagatggtg cagatgatct cgctcgcccg
    10261 tcagttcgag atgcagaccc gcatgctgtc gaccgccgag cagaacgacc gctcggccgc
    10321 gcgggtcatc ggcggccagg gctgatcgag gcgcgctccc tgccgagccg ggctgcgcgg
    10381 gccgggttgc gtggggcgcc cggcctttct tgtgcacccg gccgcttcga ttagcacggt
    10441 ttttgcgttg cttatcgcag gatggtcggt gacggcaccg gcctagactg atcccatcga
    10501 aaaagcggca gtacggcggc aatttcagct gggtattcca accccgcacg gcaaatcctg
    10561 ccgccccggc aacgcaaggc aaaggaaccc atcatgatcc gctccctgtg gaccgcccgt
    10621 accgggctgg atgcccagca aacctcgctg gacgtgatct ccaacaacct ggccaacgtc
    10681 tccacgaacg gcttcaagcg tcagcgtgcg atcttcgagg acctgctcta ccagaccatc
    10741 cgccagcctg gcgcacagtc gacccagcag acgcagatct ccagcggcct gcagatcggt
    10801 accggcgtca agccggtggc gaccgagcgc atcttcaccc agggcacatt gcagcagacc
    10861 ggtggctcgc tcgacgtcgc ggtgcagggc aacggctttt tccaggtcgc catgcccgat
    10921 ggcaccaccg cctacacccg tgacggcgct ttccagctcg acagtcaggg caacgtcgtc
    10981 accgccagcg gttacaccct ggccgacaac atcaacatcc ccgccgacgc gctctcgatc
    11041 accatcggca aggacggcac ggtcagcgtg ctgcaggccg ggcagaccgc gccggtggaa
    11101 atcggcgcca tccagctcgc caccttcatc aactcgggcg gcctgcaggc cgcgggcgag
    11161 aacctgttct acgagaccgc ctcgagcggc gtggccacgc ccacccagcc gggcaccaac
    11221 ggcgcgggcg tgctcaacca gggctatgtc gaggtctcca acgtcaacgt cgccgaggaa
    11281 ctcgtgagca tgatcgtgac ccagcgcgcc tacgagctca actcgcgcgc gatctcgacc
    11341 tccgaccaga tgctcggccg gctgacccag ctgtgaggcg cacgatgaag ctcgccaggc
    11401 attcgatcgc cgtcggcctg ctcgcgctgc tgtcgggctg cgccgcgatc cagaccaccc
    11461 cgccgtctgc ggtgcatcag ccgatgagcg tgcgccccga ggcgatggcg accgcgctgc
    11521 ccgccaatgg cgcgatctac cagaccgtgc aggcacgccc cttgttcgag gatcggcgcg
    11581 cgcgccgcat cggcgacacg atcacgatca acctggtcga gcgcaacacc gcgcagaaga
    11641 gcgccaacgc caacgccacc cgcaacggca acatgagcgc cggcatcggc cccatcaacc
    11701 gcttgccctt gagcggcctg aacggcctgg agctcgaggc cgatgccgag tccgacttca
    11761 acggcaaggg cgcggccgcg gccaacaacg tcttcaacgg cacgatcacg gtcaccgtga
    11821 tcgacgtcta ccccaacggc aacctgctgg tgtcgggcga gaagatggtg gcgatcaacc
    11881 agggcaacga gttcatccgc ttctcgggcg tgatcaaccc gaacaccgtc accaccgcca
    11941 acaccgtgca gtcgacccag gtggcggatg cgcgcatcga gtaccgcggc agcggcttca
    12001 tcgacgagtc caacaccatg ggctggctgc agcgcttctt cgtggcgatc atgccgttct
    12061 gagggccgcg ccatgtcctg tctcgcctcc cttctgctgc gcgccgcggt cgtctccctc
    12121 gccgtgctga tcttgcatcc ggcgcaggcg tacgccgcgg ccgagcgcat caaggacctc
    12181 gcctcggtgg ccggcgtgcg cgacaaccag ctcgtcggct acgggctggt cgtcggcctg
    12241 gacggttcgg gcgaccagac cacgcagacc cccttcaccg tgcagagcat cgtcaacatg
    12301 ctcggcaacc tcggcgtgag cctgccgccc ggcaccaacc tgcagctcaa gaacgtcgcc
    12361 gcggtcatgg tcaccgccaa cctgccggcc ttcgcgcgcc cggggcaggc gatcgacgtc
    12421 accgtgtcct cgatcggcaa cgccaagagc ctgcgcggcg gcacnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnccgcgc gccgggtggt cgatgcgatc aacctggtct
    12601 atcccggcgc ggcgagcgca ctcgacgggc gcagcatcca ggtgcgcgcg ccgaccgact
    12661 ccagcgcccg tgtttccttc ctcggccaca tcgagaacct cgaggtcacg cctgtgcagc
    12721 aggccgccaa ggtcatcgtc aactcgcgca ccggctctgt ggtcatgaac cagcgcgtga
    12781 tgctgcagaa ctgtgcggtc gcccatggca gcctcacggt cacggtcaac aacgaggccc
    12841 aggtcagcca gccgccgccg ctctcgggcg ggcagacggt ggtcacccag agcagccagg
    12901 tcgacatcgc ccaggagggc ggcaccttgt tcaacgtgcg cggcggcgcc aacctcgccg
    12961 acgtggtgaa ggcgctcaac acgctgggcg ccaagccgat ggacctcgtc agcatcctgc
    13021 aggccatgaa ggcggccggc gcgctgcgcg ccgagctcga ggtcatctga ggaggccgcc
    13081 atgaacaagg gcttccagct caacgcgctc gaccccaatt cgctcggcga cctcaagcgc
    13141 ctggcgcgca gcgagggcgg ctcggacgcc gccctgcgcg ccgcagccaa gcagttcgag
    13201 ggcctcttcc tgcagatggt catgaagtcg atgcgcgcga ccaccgggca gaacggcatg
    13261 atggacagcg agcagacacg ctcgtggcag ggcatgctcg accagcagat ggcgctggag
    13321 atgtcgcagt cgggcggcac cgggctgtcc gagatgctct accgccagct tggcggcaag
    13381 gctgcggtgc ggcccgacgc ggcaggcgag gcaggcaaga ccggcttcga tcttgccgac
    13441 gtgcgtcgtc gcccggcaat tcctgccgtg ctcgcgcgcg atgcggaaag tgctgccgcc
    13501 ccgcgccttg ccgccttgcc ggccgaggcg ggtgtaccgg cactgggcgc cgccgcgacc
    13561 gcaggggagt tttccgcgct gtcggtgtac gagcgtgctg cgctgcagac ccgcctcgac
    13621 gccgcggtgc gcagcgcgcg cgatgccggc ggggaggtgt cgaagtccgc gcaggacttc
    13681 gtcaccgagg tgtggccgca cgcgcaggaa gccagccgcc gcaccggcat cccggccgaa
    13741 ttcatggtcg cccaggcggc gctcgagacc ggctggggac agaagcagtt gcgtcacgag
    13801 gacggcagcc cgagccacaa cctcttcaac atcaaggccg gcagtgcgtg gtcggggcgt
    13861 accgtcgcgc gcgaggtcac cgagtacgcc gacggccagg cctacaccga ggctgcgcac
    13921 ttccgttcct acggctcgta tgccgagtcc ttccgcgact atgcccagct gatgacgcgc
    13981 agcccgcgct acgccggcgt gctcggccag accgacgcgg cggccttcgc gcgcggcctg
    14041 caggacgccg gctacgccac cgacccgatg tatgccgaca agctcacccg catcatcggc
    14101 ggcaatacct tgcgcagtgc actggcaatg gcgggctgag gaccggcggt cgaggtgcgt
    14161 atcgagcgct ggcaaggatc ttgcatgtag aggctcgaac cagcgcatca gggaaacatc
    14221 atggcgtcct tgctcaatat cggactgacc gggctcagcg cctcccaggc ctacctcaac
    14281 accaccagcc acaacatcgc caacgcggcg acgccggggt tccatcgcca gaccgtcacc
    14341 caggaagcgc gcgacccgca atacagcggc ggcgccttct tcggcaccgg taccggcgtc
    14401 accggcgtgg cgcgcagtta cagccagctg ctcgagggcc aggtgctgag cgccgacagc
    14461 cggcgtgcgg aatacgccgc ctacagccag ttcatcagcc agctcgacaa cgtgctcgcc
    14521 gacgccgaca ccggcctcac gcccgcgctc aacgaattct tcggtgcgct gcagaacgtc
    14581 acctccaacc cgaccaacgt ggccgcgcgc caggcgctgc tctccagcgg cgataccctg
    14641 tcggcgcgct tccgcaccct ggacgggcgt atctcggaga tcgcggaagg cgtcgagacc
    14701 gagatgcgca gcacgctgga gagcatcaac ggctacgccg ccgcgatcgc cgagctcaac
    14761 gagcgcatgg tgatctcgcg caccggcggc gccggttcgg ccgccaacga cctgctcgac
    14821 cagcgcgacc aggcgatcgc cgagctcaac cgcctcgtca aggtgagcac ggtggagcag
    14881 cgcgacggtt cgacgtcggt cttcatcggt tcggggcaga gcctggtcat gggcaatcgc
    14941 gccaagcagc tcgaattggg cgaccagctc gacgagaacg ggcgctcgat gatcacggtg
    15001 cgcgccaact cgggggtggt ggagccgctc gccgagagcc tcgtcggtgg tggcacgctc
    15061 ggcggcctgc tggcggcgcg ccgcgagggc acggcctcgg cgcagcgcag cctcggcttg
    15121 ctggcgacca cgctggcgac cgagttcaat agccagcacg cgctcggcct cgacctggaa
    15181 ggccggctgg ggggagattt cttcaggatc ggcggggtgc gcgtcgatcc gtcggacagc
    15241 ggcctgagcg tccggatcga ccccgggcag gtctcctcgc tcaccggcag cgactactcg
    15301 ctgcgctacg acggcgcggc cttccagctc accgatctcg ccactggcaa cgccgtgccc
    15361 gccgcgcaga tgcagctcgt gaccgaggcg gacggttcga ccaccatccg ggcgcagggg
    15421 ctcgtcttta ccggcgcgcg gagcaccctg accgagccgg ccttcgtcta tcccacccgt
    15481 tacaccgcgg gggcgatcga ggtggcgaac cgggacccgc gcgccgtcgc ggccgcggcg
    15541 ccggtgctgg ccagtccgac caggggcgac ggcagctcgc tgcggatcga gcaatccggg
    15601 ctgctcagcc gcggcagtgc ggcggccggc accgagctcg gcctgcccgc cctgaagtac
    15661 accttcaacg cggtggacaa gcgcttcgaa ctcgacgcgg ctgcgccggg ggcggccggc
    15721 tggcctgcga ccctggccta cgacccggcg accagcgccg cgggcgagtc cttcacgctg
    15781 gaggggcccg gaggcgtccg gctcgagctc aagatctccg gcacccctgc cgctaacgat
    15841 gtcatcgcgc tcgccgacaa caagagcggc gtcgccgaca accgcaacgc tgcgctgctc
    15901 ggcgcgctgc agaccgacaa gctcttgttc ggcagcggcg gccaggccac cgccacgctc
    15961 aacaacgcct atgcgcagct ggtggccaag gtcggcagca agacgcgcga ggtgcaggcc
    16021 ggcgaacgca cccagacctc gctgctcgac caggccaagg cttcgcgcga cgcggtctcg
    16081 ggcgtgaacc tggacgagga ggcggccaac ctggtgcgct tccagcagag ctaccaggct
    16141 gcggccaagg tgatgggcgt ggcgcagcgc ctgttcgacg aaatactggc gatcgcccgc
    16201 tgaggagaga gcacaccatg cgcgtatcga ccaacatgat ctacgaccag ggcgtcatcg
    16261 ccctgcaaaa gcaatccgcc gcgctgttgc ataccggcca gcagatcgcc accggccgca
    16321 agatcctcac cccttcggac gatccggtgg cctccgcgcg cgcgctcgat gtcagccagt
    16381 cgaagagcgt caaccagcag ttcatcacca accagggcta tgccgacgat gcgctcaagc
    16441 tggtcgacgg ccagctcgac ggagccggcg agatcatcca gtacgcgcgc gagcgcgtgc
    16501 tcgcggctgg caatcccgca ctgtcgccgt ccgaccgcca gtcgatcgcc tccgacctgc
    16561 gcagccagtt cgatgccatg ctggcgatcg ccaacacgcg cgacgccaac ggcgactacc
    16621 tgttcgccgg ctaccggtcg aacacgccgc cgttcgagaa gacggtcact gccggcacgc
    16681 tcggcagcga ctacaagggc gatcagggtt cgcgcaccat gcaggtctcg tcttcccgct
    16741 tcatgccgat cagctttggg ggcgagtcgg tgttcgggag cgagggcgac ggggtgttca
    16801 aggtgctggg cgagttcatc tccgccctcg aaggcggagg cgatgtgagc ggcgcggtga
    16861 gcacggcgct gggcggtctc gacgacagcc tggaccgcac gctcaccgtg cgcgcgcaga
    16921 tcggctcgca gcaggtcgag gtcgagcagc tcggcttcat cggcagcgat ctcgacctgc
    16981 agtaccagac cacgctgtcc aacctgcagg acctcgacta caacgaggcg atctcgcgcc
    17041 tgacccagca gcgcaccttc ctcgaagccg cccagcagtc cttcctcaag acgaccgggc
    17101 tgtcgctgtt caactacctc agctgaccgc catgcgccgc gcgctcctcg tccttgccgc
    17161 ctgttccgtc ctcggctgcg ccagcgagcg cgagggcacc ggcctgctcg accgcgccgc
    17221 cgcggcccca gggccgatcg ccggtgcggt gggtgcggtg tggatggcga gcgagctttc
    17281 ctacctcgag ccggagtacc tcgccggcat gctggtggcc tacggcatct acgatccgct
    17341 cgcgccgacg tggtcgatcg aggtcagccg cagcggcgaa gaccgtgtgc gcatggacct
    17401 gcacatgaag cggatgacca ccggcggcga cggcgaggca cgccgggtct tcctgcgcaa
    17461 cgcgcgcgag atcgtgcgtg cgggcggttt cgccggcttc gacgagctga gctgggagga
    17521 gggcatcgag tccacccggc ctttcgcccg ccgcgtcgcc agcggcgaga tccgcctggt
    17581 gcgttcgcgc accttcccgg agttctgagc gcggccctct cagggtgtgg ggcgtggcgc
    17641 gagcgcgtcg agcagcaggc cgatggtgtc gtagctgcgc tcgaagaggc tttgcagcac
    17701 cggcgcgaag ttgctcatga tcagcagcac gccgatgaag cccgcggtca gcgtgatcgg
    17761 aaagcccacc gcgaagaggt tgagctgcgg cgccgcgcgg gtcagcacgc cgagcgcgat
    17821 gttggccacc agcaagaccg cgatcaccgg cagcgacagc agcagccccg ccgagaacac
    17881 cgtgatgccg ctgcgcgcga ggaaggccca gccgcctgca tccaccgcat cggtcgccac
    17941 cggcagccac tcgaagctgc gcaccaggag gtcgatcatc agcaggtggc cgtcgatggc
    18001 gaggaagatc agggtcgcga gcaggttgag gaagtcggac agcaccgcgg tctgcccgcc
    18061 cgcgtcgggg tcgaagaaga ccgcgaacga gaggcccatc tgcatcccga tcagggcgcc
    18121 ggccatgtcc accgcagcga atacgatgcg catcacgaag ccgatggcga tgccgatcag
    18181 gatctgctgc gcgaagatcg ccaggcccag tccgccgccg ggcggcacct gcggcatcgg
    18241 cggcaggacc ggcagcaccg ccatcgccac gcccagcccg atcgccaggc ggatgcgcac
    18301 cgacacgcca cggttggaga acaccggcgc cgacgcgaac agcccgagca ggcgcccgag
    18361 cgggaacatc agcgccgcca gccaggcgtc gagctgggct gcggtgacgg agagcatggt
    18421 ggcggggaag ggcgaggggc ggggaggagg ctcagccgat catcgccggg atcgactcga
    18481 acagccggcg gatgaagtcg gtgagcaggg tgatcatcca cggcccggcc agcaccaggg
    18541 tcacgaagat cgccaccagc ttgggcacga aggagagcgt catctcgttg atctgggtgg
    18601 cggcctgaaa gatgctgatg atcaggccgg tgacgagcgc ggcgaggaag agcggcgccg
    18661 acaccatcag ggtgacctcg atggcctggc ggccgagctc gatgacggtg gtgggggtca
    18721 tggcgtttgt cctcgggcgg gcgcggtcac accgcgaagc tctgcaccag cgagccgatc
    18781 agcagcgtcc agccgtcgac cagcacgaac agcatgatct tgaaaggcag cgacacgatc
    18841 accggcgaca tcatcatcat gcccatggac atcagcaccg aggccacgac catgtcgatg
    18901 atgatgaagg ggatgaagac gatgaagccg atctggaagg cggtcttgag ctcggaggtg
    18961 acgaaggcgg ggatcaccac ccgcatcggc aggtcctcgg ccttgtccac cggcggcagc
    19021 ttggccagcc cggcgaagag cgcgaggtcg ggctcgcgca cctgcttgag catgaagccc
    19081 ttgaccggca ccgaggcgcg ctcgagcgcc tcgtcgaact ggatgcggcc ttcggacatg
    19141 ggcagatagg cctccgtgta tacgcgctcg ccgaccggcg ccatcacgaa gaaggtgagg
    19201 aagagggcga ggcccagcag cacctggttg ggcggcgcgg tctgcgtgcc catggcctgg
    19261 cgcagcagcg agaacacgat gacgatgcgg gtgaagcagg ttgtcatcag caccaacgcc
    19321 gggatgaagc tcaggagcgt catcagcatt agcgtctgga tggtcaggct gtaggtcgtg
    19381 ccgccgcccg ccgccgggct ggtggtgagc gcgggcaggg cctgcgtgtc cagcgccggc
    19441 cacaagaagc gaccctggtt gaggcggcgc gtcgcacacc agacaccgaa gccgtcgtgc
    19501 acgacaagct tcacccgcgt gccgcgcgcg ttggcgaaga ggtagccgtg atgcgcttgc
    19561 gcgcggccga acacctgcac cgggtccttg ccagtggtcc acagctcgtg attgccgggc
    19621 acccagatca ccttggcgaa gcgccggcgc agcagatcca gcgaccagcg gatgtcgtcg
    19681 gtgcgctcgg caacgtcgcc ggcgacgatc agccagtcgt cc
//
LOCUS       DAOQNN010000017        19003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1068_length_19003_cov_10.032088, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000017 DAOQNN010000000
VERSION     DAOQNN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 19003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19003
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1068_length_19003_cov_10.032088"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /gene="bcrB"
                     /locus_tag="PKV97_02295"
     CDS             complement(<1..1064)
                     /gene="bcrB"
                     /locus_tag="PKV97_02295"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HNB04734.1"
                     /translation="MSAATSPEVIKEPSMVKQKEMIAGNYDRLTGTKESGEKVVSTFV
                     PGNLNELIMCFDMVNNLPETNAIQNGMRKVSGGMIMDAEKAGHSEDVCTYVKADIGMM
                     GRGNIAPNGKPMPAPDMLLLSYTGCFTFMKWFELLRHEYKCPTVMLQIPYQGDGKITK
                     NMRDFVVKQLKEEVIPMFEKVSGVKFDIDRLREYLKNSAKAEDNLVWVLESAKNRPSP
                     IDAYFGGVYYIGPMFTAFRGTTDAVEYYDLVRKEIEQRIREGKGPITPEGDMKEEKYR
                     LVVEGPPNWTSFREFWKLFYDEGAVVVASSYTKVGGLYDQGFRHDPNDPLGTLADYCL
                     GCYTNNNLPQRVELLEKYMN"
     gene            complement(1067..2227)
                     /gene="bcrC"
                     /locus_tag="PKV97_02300"
     CDS             complement(1067..2227)
                     /gene="bcrC"
                     /locus_tag="PKV97_02300"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit C"
                     /protein_id="HNB04735.1"
                     /translation="MSTADIIARCEALYEDLDFTAARKWKEADPSRKVIAYMPVYVPR
                     EIIHAAGMLPLGIMGGGDGLEVIHGDAYYQSYICRIPRSTIELGLSKRMDFVDGMLFP
                     SICDVIRNLSGMWKLLFPGKYVRYFDVPQNYRDDVGGSYYTNELNELREGLEHLSGRK
                     ITDDALRASIAVYNENRRLVQAVYGMRSREPWKVPSSEVYLLMRAGLVLPVEEHNQML
                     SEYLAAAENVKASKRDNCRVVINGSFCEQPPLNLIKSIELAGCYIVDDDYMIVHRWLR
                     NPVSTGGDPMQNLSLAFLHESIATAAKYDDKEEDKGKYLLEQVRSNAAEGVIFAAPSF
                     CDPALLERPMLADRCSEHNVPYISFKYAENSGQMQPIREQAGTFADSIKLWS"
     gene            complement(2230..3006)
                     /locus_tag="PKV97_02305"
     CDS             complement(2230..3006)
                     /locus_tag="PKV97_02305"
                     /EC_number="4.2.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexa-1,5-dienecarbonyl-CoA hydratase"
                     /protein_id="HNB04736.1"
                     /translation="MGEAINPLKAWLERDGGLLRLRLARPKANIVDAAMIGALQQALG
                     AHLGSPALRAVLLDAEGPHFSFGASVDEHMPEQCAQMLKTLHALVRQMLDSPVPILVA
                     IRGQCLGGGLEVAAAGNLLFVAPDARLGQPEIRLGVFAPAASCLLPPRVGQSRAEELL
                     FSGRSIDGAEAHRIGLADVLADDPEAAALKWYDEHLAALSASSLRFAVRAARCDRVPA
                     IKQKLETVEAMYLEELMASHDAVEGLRAFLEKRSANWENR"
     gene            complement(3006..4139)
                     /gene="oah"
                     /locus_tag="PKV97_02310"
     CDS             complement(3006..4139)
                     /gene="oah"
                     /locus_tag="PKV97_02310"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HNB04737.1"
                     /translation="MKETTQRVIDQTAPKHLVDHNLVPETVVPGVIYEKRPARNLQGE
                     VVPGLYNAWIWLDNPKQYNSYTTDMVKGLILAFRAASCARDVATVVFTAVGDKAFCTG
                     GNTKEYAEYYAGNPQEYRQYMRLFNDMVSAILGCDKPVVCRVNGMRIGGGQEIGMAAD
                     FTVAQDLANFGQAGPKHGSAAIGGATDFLPVMIGCEQAMVSGTLCEPFSAHKAYRLGI
                     SSQIVPALKIDGKFVANPLVITDRYLDEFGRIIHGEFKTGDELAAGKELLKRGEIDLS
                     LLDEAVEKLCAKLISTFPECLTKSFEELRKPKLDAWNRNKENSRAWLALNMMNEARTG
                     FRAFNEGTKETGREIEFTDLRQALAVGTPWTPELIESLMPGAK"
     gene            complement(4192..5259)
                     /gene="had"
                     /locus_tag="PKV97_02315"
     CDS             complement(4192..5259)
                     /gene="had"
                     /locus_tag="PKV97_02315"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HNB04738.1"
                     /translation="MSTKPYRWMMVEPGRPMVRTEFEIGELAADQVVVEVAGCGVCHT
                     DLGYYYDGVRTNQPLPLALGHEISGRVVGAGSAAGQWVGRAVVVPAVMPCGTCELCTS
                     GHGTICRAQVMPGNDIQGGFASHIVVPARGLCAVDEKRLAAAGLQLADVSVVADAVTT
                     PYQAVTQAGVEPGDVAIVIGVGGVGGYAVQIANAFGASVVAIDVDPVKLELMSKYGAA
                     LTLNAREVTGRDLKKAIEAHAKANGLRTTRWKIFECSGSGAGQTNAYSLINHGATLAV
                     VGFTMDKVEVRLSNLMAFHARALGNWGCLPEYYPAALDLVLDGRINLANFVERHPLAQ
                     INEVFEAAHAHKLSRRAILTP"
     gene            5578..7296
                     /locus_tag="PKV97_02320"
     CDS             5578..7296
                     /locus_tag="PKV97_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNB04739.1"
                     /translation="MTARSVSITFAGSGGAGVMTAGNMLLDAAGRAGWYAYMTRSSGA
                     QIRGGEAAAMMRLSTSPVQSHDDQYDLLVAIDWENVGRFSAEIPMTADSLVVGDPDGG
                     EFPEAIRAKGTRSADIPFKKMAKTIEGGRPNMIALGAVAGLVGLPEDAVLGVVRDSLA
                     KKGEAARTASEASVRAGMAFAADLPPCPRLATAQGQSERLWSITGNEAAGLGAVRGGI
                     RFVAAYPITPGTEVLEWLAPNLAKLGGVLVQAEDELASINQIIGASYAGVPSLTATSG
                     PGLALMTESLGLAVASETPITVVNVMRGGPSTGIPVKSEQSDLNIALYGLHGDAPHLV
                     VAPNSLADCAFATQWAVHLADTLQTAAIVLSDQSLGQSRATISPPADPGLRAVRLMPE
                     GEAAERYRRYTNTASGVSPMAVPGMKGYQYTADGLEHNEFGTPSSGAADHSAQLDKRL
                     RKLALHDYGTHWADIEGDGDIAVLTWGSTTGPVREALERFRASGGRARLVSIRLISPV
                     RPEQLAAALAGVARVLVVEQSHGAQFHRYLRAHYDLPGSVRAFHRPGPLPIRPNEIFR
                     QLADWS"
     gene            7299..8138
                     /locus_tag="PKV97_02325"
     CDS             7299..8138
                     /locus_tag="PKV97_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNB04740.1"
                     /translation="MEATSTCPSYSARDYKSEVKPVWCPGCGDYSVLGSITKALAELS
                     IPPERVALVSGIGCSSRLPAYTNVFGFHGVHGRALPIATGIKVSRPELTVIASGGDGD
                     GFSIGGNHFMHACRRNVDMTYIVMDNEVYGMTKGQASPTTAPDWEKSKLTPQGTGINP
                     FHPLVIALAAGANFIARGFSGDQNGTAKLITEAIRHPGFSFVQLLSPCVTFRPEQRDW
                     KDMVHPAAVDPTDDPARAARRLMSDDGFNVGVLFRGSRKPFQPGQPASVDSVADLEKE
                     FVL"
     gene            8135..8674
                     /locus_tag="PKV97_02330"
     CDS             8135..8674
                     /locus_tag="PKV97_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNB04741.1"
                     /translation="MSKPDPQETGIHSVEHLLAHALAMEVEAVERYEALADQMETHNN
                     RDVAVLFRKLAEIEKLHVDNVNDISEGHTLPHIAPWEYSWEAPESPEAPSVSAEGLHY
                     MMHPYQAIAMALEAERRGVDFYDRLVAQSEREDIRKLAAELCETEREHVTLLEGWLAR
                     FQPPPKGWNEDPDPPLLQG"
     gene            complement(8921..9574)
                     /locus_tag="PKV97_02335"
     CDS             complement(8921..9574)
                     /locus_tag="PKV97_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HNB04742.1"
                     /translation="MFVENIMTREVLHVGPEASFSQVSEIMRLKKVRHVPVIDQDRKV
                     LGIISHRDVQRAQPSMITTLDVGEVKYLLSKITAADIMHKSVVSCSPRTQIEEAARMM
                     RPKKLGCLVVVDDAGRLVGIVTSVDLLDFFLDITGCWVEGATRITVKLQDRAGQLAAL
                     LASVNAQGGYIVSVVSPRTPQSEGLRTATIRFEAKDAGAVVQGLREAGYELSVDQTA"
     gene            9920..12661
                     /locus_tag="PKV97_02340"
     CDS             9920..12661
                     /locus_tag="PKV97_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNB04743.1"
                     /translation="MTVSSPQGTTKVATYCYQCVAGPDLLKVKVEDGVATAVEPNFDA
                     EDVHPAAGRVCVKAFGLVQKTYNPNRVLQPMKRTNPKKGRHEDPRFVPISWDEALDTI
                     AAKLRGIRETGLLDASGYPRVAASFGGGGTPTAYMGTFPALLAAWGPVDMSFGSGQGV
                     KCYHSEHLYGELWHRAFIVCPDTPRSKYILSFGSNIEASGGVCGVWRHAAARVEQGVK
                     RVQVEPHLSVTGGCSAEWLPIKPKTDAACMHAMIHVMLFENARSRLDIDFLKHMTASP
                     YLVAPNGFYLRDPDTRKPLVWDLKSGKAVVFDTPGIDPALDGDFLASGLEVLPDQELV
                     THEAAPVRSAFGKLLDHERTFTPEWAQGICDVPAATIRRIANEYLDHAEIGATIEIEG
                     RTLPYRPVAVTLGKTVNNGWGGYDCCWARTLMACLVGALDVPGGTIGTAVRLNRPAND
                     RQSSAKPGVDGFMDYPFNPTDKENWISRPQIRNANRTLVPLVANSAWSAALGPTHLAW
                     MQQRHGFESFPEPTQPDVWFFYRTNPVISFWDTPQVAEAVAKFPFVVAFTYTRDETNH
                     FADILLPDCTDLEGLQMLRIGGTKYVEQFWDHQGFALRQPVVPTQGDTRDFTWISTEL
                     ARRAGILEPYNKAINRGAAGVPLKGPNYDFSLQPDHAYGVEEIWDASCRAASAELTEG
                     AEDHGLEWWKEHGFRTIEYPRLQWYLYPHLVDQGLRFEMPYQERIYRIGEELGRRLHE
                     SGIDWWDRQLTEYRPLPEFHDFSHLIKHAVISNLGGRDEDFPFWLLTARSMQYSWGGN
                     VSLQMVREVAANVKGHRGVIMNPTAARKLGIEDGDLVEVRSPLRETRGRVVLRQGIRP
                     DTLLMVGQFDHWITPYAKDFDVPSMNALVPMLMDLTDATGSAADIVPVSIKRIGGAQ"
     gene            12658..13308
                     /locus_tag="PKV97_02345"
     CDS             12658..13308
                     /locus_tag="PKV97_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB04744.1"
                     /translation="MTRYAMVVDLRRCVGCQTCTAACKHTNATPPGVQWRWVLDVEAG
                     EFPDVTRSFVPVGCQHCDEPPCETVCPTTATKKRADGLVTIDYDLCIGCAYCSVACPY
                     NARYKVTRDTPAYGEGKMANEVKRTDPARIGVATKCTFCSDRIDYGHAHGLVPGVDPD
                     ATPACANACIASALTFGDIDDPDSKASRLLRENEHFRMHEELGTGPGFFYLWEKKQ"
     gene            13305..14186
                     /locus_tag="PKV97_02350"
     CDS             13305..14186
                     /locus_tag="PKV97_02350"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HNB04745.1"
                     /translation="MRGVEPELQPFWDERAAGNFIGGGTGAGLLLWSAIAASGHGTAI
                     GLPLFLALAFIGVGLFCVWMEIGRPLRALNVFFHARTSWMTREAIVAGPLFLCGLIAL
                     LSGWLFFAWLAALIGLVFLYCQARILQAARGIPAWREPALLPLIVVTGLAEGAGMLAL
                     LSPLLVRSPGAEFAFPLLVLVVLRFWLSRRYAAHLARPNNAPLKTLRALRENDRHFLL
                     LGHTLPAAVLLIALFAGGSFGTALLALAGIAVAASGWLFKLALITRIAYTQGFALQHV
                     PARTPGYSHPGVKPGWN"
     gene            14252..14824
                     /locus_tag="PKV97_02355"
     CDS             14252..14824
                     /locus_tag="PKV97_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNB04746.1"
                     /translation="MYEVRIHGRGGQGAVLAAQLLATALVFDGKYAVSIPAFGFERRG
                     APVVSFIRASEQPIRQLTNIYAPDCIVCVDPALARTVNIFAGLKPGGTLVQATSRPLA
                     EIDVPDSVGTVGLCDAVRIATEIFGRPITNTLMLGAFARSTGQVSLEALKKVIEQSDF
                     RDAGLRQNLDALERGYAETTVHTLERRITA"
     gene            14821..15123
                     /locus_tag="PKV97_02360"
     CDS             14821..15123
                     /locus_tag="PKV97_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB04747.1"
                     /translation="MSERHQHYPAWHLKEAGVPDDLCPVATEQSPMLPGDWRSFRPVV
                     DRDKCVKCAVCWLYCPVQCVEEHAAWFDFDMKTCKGCGICAHECPQRAITMIPEAQ"
     gene            15120..16376
                     /locus_tag="PKV97_02365"
     CDS             15120..16376
                     /locus_tag="PKV97_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylglyoxylate dehydrogenase"
                     /protein_id="HNB04748.1"
                     /translation="MNTTTLDTAPTTEKRPAPQLMLCEGNEAAALGVALARPDMVAVY
                     PITPQTSLVEKLAKLIADGRMDADIVDAEGEHSVLSVLQGGALAGARTYTATCGPGLA
                     FMFEPYFRTSGMRLPLVMTIVTRDGITPQSVWGGHQDAMTVREAGWIQMYCETVQEVL
                     DTTVMAFRLAEHHDVMLPVNVCLDGNYLSYGTSRIEMPDQAEVDDFMGHKDVNWHVAL
                     DPLRPMAVDPLTGGSGGNGPETFVRYRRGQCAGMKNALHVITEMHEDWARRFGEAHRF
                     APLVEEYRLDDAEYAIMTLGSMTGAAKDAVDEARAAGEKVGLIKIKTFSPFPVEALQH
                     ALRGVRALGVVDRSVNFRWNCGPMFQETLGVLYRLGRQVPAQSFIGGLAGADLTVTTF
                     RRVIDETRALLERAPSDEPVWLNAKD"
     gene            16401..>17041
                     /locus_tag="PKV97_02370"
     CDS             16401..>17041
                     /locus_tag="PKV97_02370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNB04749.1"
                     /translation="MHTTDYLIAGSSHAALEAINAIRMHDGEGSITVVTRDPHRPYSP
                     TVLPYVVSGRSTPERVFLRDDDFFTANKVDYRSGCALAGLDPAAHQARLADGSTIGYR
                     KLLLATGASPIVPPIPGIERVDYHVLRTLDDATRLRAAMAASKNAVVLGAGLVGMHAA
                     ENLVKAGASVTIVEMSKQLTSGYFDEVAAGMIEQAFLGNGAKIRTGHRVVGLEP"
     assembly_gap    17042..17141
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17142..17437
                     /locus_tag="PKV97_02375"
     CDS             <17142..17437
                     /locus_tag="PKV97_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB04750.1"
                     /translation="PLNTYHFFGRHAISVGSAAVPEGGRAQVRFDAASGRYLRAVFDR
                     DERLTGIFGVNEFFDAGVMAQLILRRTDLGPLRERFFAQPLATGRELMSQLWR"
     gene            17448..18803
                     /locus_tag="PKV97_02380"
     CDS             17448..18803
                     /locus_tag="PKV97_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNB04751.1"
                     /translation="MGQAYSTIAFDPAKCDGCGKCMTACASVKFDSTDTTRSLIQIVR
                     SGGARIEPPRPGEFELALCRQCADPKCVTVCPAGALGKNGATGVIDWDASKCVDCLLC
                     TIGCTYAGIALDEHSGRVSKCDTCEGKPACVPACPTGALRHITTARIYNEVGSWEDLF
                     APGLAGCQGCNTELLMRHALRRVGPDTVLATPPGCVPGMGSVGFNGTTGTKVPVFHPL
                     LTNTASMLAGVKRQYERVGRKVTAMAIAGDGGASDVGFQSLSGAAERGERILFMVVDN
                     EGYMNTGMQRSSCTPYGAWTSTTPIGAHGRGKTQDAKNLPMIMVNHRCEYVATASTAY
                     MEDLYEKLDKALAASERGFAYLHVYSPCTTGWRFQSALNIEVARKAVESNFVTLWEYT
                     PEAGLHFTRPVDKPLPVADYLKAMGRFRHLSPEQVEHIQNKVAENLRFVRKIAEQADE
                     Q"
     gene            18793..>19003
                     /locus_tag="PKV97_02385"
     CDS             18793..>19003
                     /locus_tag="PKV97_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNB04752.1"
                     /translation="MSNEKPSPPEQMSRAELQALQLERMRWSLQHAWDNVPHYRHAFE
                     AAGVRPSDLRGLADLARFPFTAKQDL"
BASE COUNT         3088 a         6796 c         6055 g         2964 t
ORIGIN      
        1 tcgttcatgt acttctcgag cagttcgacg cgctgcggca ggttgttgtt ggtgtagcaa
       61 cccaggcagt agtcggccag cgtgcccagc ggatcgttcg ggtcgtgacg gaagccctgg
      121 tcgtagaggc cgcccacctt ggtgtaggag ctcgccacca ccaccgcgcc ctcgtcgtag
      181 aagagcttcc agaactcgcg gaagctggtc cagttcggcg gaccctcgac gaccaggcgg
      241 tacttctctt ccttcatgtc gccttcgggc gtgatcgggc ccttgccctc gcggatgcgc
      301 tgctcgatct ccttgcgcac caggtcgtag tactcgaccg cgtcggtggt gccgcggaag
      361 gcggtgaaca tcgggccgat gtaatacacg ccgccgaagt aggcatcgat cggcgaggga
      421 cggttcttcg cgctctccag cacccagacc aggttgtcct cggccttggc cgagttcttc
      481 aggtattcgc gcagacggtc gatgtcgaac ttgacgcccg agaccttctc gaacatcggg
      541 atgacttctt ccttgagctg cttgaccacg aagtcacgca tgttcttggt gatcttgccg
      601 tcgccctgat acgggatctg cagcatcacc gtcggacact tgtactcgtg gcgcagcagc
      661 tcgaaccact tcatgaaggt gaagcagccg gtgtaggaca gcagcagcat gtccggcgcg
      721 ggcatcggct tgccgttggg cgcgatgttg ccgcggccca tcatgccgat gtcggccttg
      781 acgtaggtgc acacgtcctc ggagtggccc gccttctcgg cgtccatgat catgccgccg
      841 gaaaccttgc gcatgccgtt ctggatggcg ttggtctcgg gcaggttgtt gaccatgtcg
      901 aagcacatga tcagctcgtt caggttgccc gggacgaagg tcgacacgac cttctcgccc
      961 gactccttgg tgccggtgag acgatcgtag ttgccggcga tcatctcctt ctgcttcacc
     1021 atcgagggtt ctttgatgac ttcgggggag gtggctgcgc tcatgattag ctccagagtt
     1081 tgatggagtc ggcgaaggtg ccggcctgct cgcggatcgg ctgcatctgg cccgagtttt
     1141 ccgcgtactt gaacgagatg taggggacgt tgtgctcgga gcagcggtcg gccagcatcg
     1201 gccgctcgag cagggccggg tcgcagaagc tgggcgcggc gaagatgaca ccctccgcgg
     1261 cgttgctgcg aacctgctcg agcaggtact tgcccttgtc ctcttccttg tcgtcgtact
     1321 tcgcggcggt ggcgatcgac tcgtgcagga aggcgagcga caggttctgc atcggatcac
     1381 cgccggtcga gaccggattg cgcagccagc gatgcacgat catgtagtcg tcatcgacga
     1441 tgtagcagcc ggcgagctcg atcgacttga tcaggttgag cggcggctgc tcgcagaacg
     1501 agccgttgat gaccacgcgg cagttgtcgc gcttggatgc cttcacattc tcggctgcgg
     1561 cgaggtattc gctcagcatc tggttgtgct cttccaccgg cagcacgagg ccggcgcgca
     1621 tcagcagata gacttcggac gagggcacct tccacggctc gcgggaacgc atgccgtaca
     1681 ccgcctgcac cagcctgcgg ttctcgttgt agaccgcgat cgaggcacgc agcgcgtcgt
     1741 cggtgatctt gcgcccggag aggtgctcga ggccttcacg cagttcgttc agctcgttgg
     1801 tgtagtagct gccaccgacg tcgtcgcgat agttctgcgg cacgtcgaag tagcgcacgt
     1861 acttgcccgg gaacagcagc ttccacatgc cggacaggtt gcggatcacg tcgcagatgc
     1921 tgggaaacag catgccgtcg acgaaatcca tgcgcttgga cagccccagc tcgatggtcg
     1981 agcgtgggat gcgacagatg tagctctggt agtaggcgtc gccgtggatc acctccaggc
     2041 cgtcgcctcc gcccatgatg ccgagcggga gcatgcccgc ggcgtggatg atctcgcgcg
     2101 gcacatacac cggcatgtag gcgattacct tgcgcgaggg gtcggcctcc ttccacttcc
     2161 ttgcggcggt gaaatcgagg tcttcgtaga gcgcttcaca gcgcgcgatg atgtctgccg
     2221 tactcatggt caacggttct cccagtttgc agaacgcttc tcaagaaagg cccgtagacc
     2281 ttccaccgca tcgtggctgg ccatcagctc ctcgagatac atggcctcca cggtttccag
     2341 tttttgcttg atcgccggca cccggtcgca gcgtgcggcc cgaaccgcga aacgcagcga
     2401 gctggcgctc agcgcagcaa gatgttcgtc gtaccacttc agcgcggccg cctcggggtc
     2461 gtcggcgagc acgtcggcca gcccgatccg gtgcgcctcg gcgccgtcga tgctgcgccc
     2521 cgagaacagc agctcctcgg cacgcgattg cccgacgcgc ggcggcagca ggcaggaggc
     2581 cgcaggcgcg aacacgccga ggcggatctc cggctgcccc aggcgcgcat ccggcgccac
     2641 gaagagcagg ttgcccgccg ccgccacctc gagcccgccg ccgaggcact ggccgcggat
     2701 cgcgaccagg atcggcaccg ggctgtcgag catctgccgc accagcgcat gcagggtctt
     2761 cagcatctgc gcgcactgct cgggcatgtg ctcgtccaca ctggcgccga agctgaaatg
     2821 cgggccctcg gcatcgagca gcaccgcgcg cagcgcgggc gaaccgaggt gggcgccgag
     2881 cgcctgctgc agcgcgccga tcatcgcggc atcgacgatg ttcgccttgg gccgcgccag
     2941 gcgcaggcgg agcaggccgc cgtcgcgctc cagccaggcc ttgagcgggt tgatcgcctc
     3001 gcccatcact tcgcccccgg catcaggctt tcgatcagct cgggcgtcca gggcgtgccg
     3061 acggcgagcg cctggcggag gtcggtgaac tcgatctcgc ggccggtctc cttggtaccc
     3121 tcgttgaagg cgcggaagcc ggtgcgggcc tcgttcatca tgttgagcgc gagccaggcg
     3181 cggctgttct ccttgttgcg gttccaggca tcgagcttgg gcttgcgcag ctcctcgaag
     3241 ctcttggtca ggcactcggg gaaggtggag atcagcttgg cacacagctt ctcgaccgcc
     3301 tcgtcgagca gggaaaggtc gatctcgccg cgcttgagca gctccttgcc ggcggccagt
     3361 tcgtcgccgg tcttgaactc gccgtggatg atgcggccga actcgtcgag gtagcggtcg
     3421 gtgatgacca gcgggttggc gacgaacttg ccatcgatct tgagcgcggg gacgatctgc
     3481 gaggaaatgc cgaggcggta ggccttgtgc gccgagaacg gctcgcacag ggtgccggac
     3541 accatcgcct gctcgcagcc gatcatcacc ggcaggaagt cggtcgcacc gccgatcgcg
     3601 gccgagccgt gcttggggcc ggcctggccg aagttggcca ggtcctgggc gacggtgaag
     3661 tccgccgcca tgccgatctc ctggccgcca ccgatgcgca tgccattgac gcggcacacc
     3721 accggcttgt cgcagcccag gatcgccgac accatgtcgt tgaaaagacg catgtactgg
     3781 cggtattcct gcgggttgcc ggcgtagtac tcggcgtatt ccttggtgtt gccgccggtg
     3841 cagaaggcct tgtcgccgac cgcggtgaac accaccgtgg cgacgtcgcg ggcgcaggac
     3901 gcggcacgga aggcaaggat caggcccttc accatgtcgg tggtgtagga gttgtactgc
     3961 ttggggttgt ccagccagat ccaggcgttg tacaggcccg gcaccacctc gccctggaga
     4021 ttgcgcgcgg ggcgcttctc gtagatcacg cccggcacca ccgtctcggg caccaggtta
     4081 tggtcgacca agtgcttggg cgcagtctga tcgatcacgc gttgcgtcgt ttctttcatc
     4141 tcgtatggct cctctgtctg ctctcttgtc ggattggtag ggggtgcggg atcagggggt
     4201 caggatcgcg cggcggctga gcttgtgcgc gtgagccgcc tcgaagacct cgttgatctg
     4261 cgcgagcggg tggcgctcga cgaaattggc gaggttgatg cggccgtcga gcaccaggtc
     4321 gagcgcggcc gggtagtact cgggcaggca gccccagttg ccgagcgcgc gggcatggaa
     4381 ggccatcagg ttggagaggc gaacctcgac cttgtccatc gtgaagccga ccaccgcgag
     4441 cgtcgcgccg tgattgatca ggctataggc gttggtctgg ccggcaccgc tgcccgagca
     4501 ttcgaagatc ttccagcgcg tggtgcgcag gccgttggcc ttggcgtgcg cctcgatcgc
     4561 cttcttgagg tcgcgtccgg tcacctcgcg cgcattgagc gtcagcgccg caccgtactt
     4621 cgacatcaat tcgagcttga ccggatcgac gtcgatcgcc accacgctcg caccgaaggc
     4681 gttggcgatc tgcaccgcgt agccgccgac gccgcccacc ccgatcacga tcgcgacgtc
     4741 gccaggctcg accccggcct gcgtgaccgc ctggtagggc gtggtgaccg cgtcggccac
     4801 caccgacacg tcggcgagtt gcagtccggc ggcggcgagg cgcttctcat cgaccgcgca
     4861 caggccgcgc gccggcacca cgatatggga ggcgaagccg ccctggatgt cgtttccggg
     4921 catcacctgg gcgcggcaga tggtgccgtg tcccgacgtg cagagctcgc aggtcccgca
     4981 cggcatcacc gccggcacga ccaccgcgcg gccgacccac tgccccgccg cgctgcccgc
     5041 gcccaccacc cggccgctga tctcgtggcc gagcgcgagc ggcagcggct ggttggtgcg
     5101 cacgccgtcg tagtagtagc cgagatcggt gtggcagacg ccgcagccgg cgacctcgac
     5161 caccacctgg tcggcggcca gctcgccgat ctcgaactcg gtgcggacca tcggccgacc
     5221 cggctccacc atcatccagc gatacggttt ggtgctcatc tacccactcc ttccgtttac
     5281 gttcgcgtca actttgatcg atctgattgg cgactggatc gactgcttcc tgccgccgcg
     5341 ttggatgcaa agttaaatca ggccggcagg agggcagttg accaaagtca acagattgac
     5401 gtttgcgtca acgtaaactt cgacacatcg gaggtgagcg ctgcgaaggc ggcgctgcaa
     5461 cccaaccagc ctccggtcgg cgccgtgccc gcttcacccg agggggacgc gcgcatgggc
     5521 cctgaacagg accgcgccgg cacccgccgg tcgaaggtcg aacattggag aacgaggatg
     5581 acagcccgat cggtatcgat aacattcgcc ggcagcggcg gcgcgggggt gatgaccgca
     5641 ggcaacatgc tgctcgacgc cgccggccgt gccggctggt atgcctacat gacccgctcg
     5701 tcgggcgcgc agatccgcgg cggcgaggcc gccgcgatga tgcgtctgtc tacgtcgccg
     5761 gtgcagtcgc acgacgatca atatgatctg ctggtcgcca tcgactggga gaacgtcggg
     5821 cgcttctccg ccgagatccc gatgaccgcc gatagcctgg tcgtcggcga tcccgacggc
     5881 ggcgaattcc ccgaggccat ccgcgccaag ggcacacggt cggcagacat cccgttcaag
     5941 aagatggcca agacgatcga gggcgggcgc cccaacatga tcgccctggg cgcggtcgcc
     6001 ggcctcgtcg gccttcccga ggacgccgtg ctcggtgtgg tccgcgactc gctcgccaag
     6061 aagggagagg ccgcgcgcac cgccagcgag gcatcggtgc gtgcaggcat ggccttcgcg
     6121 gccgacctgc cgccctgccc ccgcctggcc accgcccagg gccagagcga gcgcctgtgg
     6181 agcatcaccg gcaacgaggc cgccggtctc ggcgcggtgc gcggcggcat ccgcttcgtc
     6241 gccgcctatc cgatcacccc gggcaccgag gtgctggagt ggctcgcacc caacctcgcc
     6301 aagctcggcg gcgtgctggt gcaggccgag gacgagctcg cctcgatcaa ccagatcatc
     6361 ggcgcttcct acgccggcgt gccctcgctg accgccacct ccggccccgg cctggccctg
     6421 atgaccgaat cgctcggtct cgccgtcgcc tcggagaccc cgatcaccgt ggtcaacgtg
     6481 atgcgcggcg gcccctccac cggcatcccg gtcaagtccg agcagagcga cctcaacatc
     6541 gcgctctacg gcctgcacgg cgacgccccg cacctggtgg tggcgcccaa ctcgctggcc
     6601 gactgcgcct tcgccacgca gtgggcggtg cacctcgccg ataccctgca gaccgccgcg
     6661 atcgtgctct ccgaccagtc cctcggccag agccgggcga cgatctcgcc gcccgccgat
     6721 cccggcttgc gcgcggtccg cctgatgccc gagggcgagg cggccgaacg ctaccgccgc
     6781 tacaccaaca ccgcgtcggg cgtctcgccg atggccgtac ccggcatgaa gggctaccag
     6841 tacaccgccg acggcctcga gcacaacgag ttcggcacgc cgtccagcgg cgcggccgac
     6901 cacagcgctc agctcgacaa gcgtctgcgc aagctcgccc tgcacgacta cggcacgcac
     6961 tgggccgaca tcgagggcga cggcgacatc gccgtgctga cctggggttc caccaccggg
     7021 ccggtgcgcg aggcgctcga gcgcttccgc gccagcggcg ggcgtgcccg cctggtgtcg
     7081 atccgcctga tctcgccggt gcgcccggaa cagctcgccg ccgcgctcgc gggcgtggcg
     7141 cgcgtgctcg tggtcgagca gagccatggc gcccagttcc accgctacct gcgcgcccac
     7201 tacgacctcc cggggagcgt gcgcgccttc caccggcccg gcccgctgcc gatccgtccg
     7261 aacgaaatct tccgccaact ggccgactgg agctgaacat ggaagccaca tccacctgcc
     7321 cgagctactc cgcgcgcgac tacaagtccg aggtcaagcc cgtctggtgt ccgggctgcg
     7381 gcgattactc ggtgctcggc tcgatcacca aggccctcgc cgagctgtcg atcccgcccg
     7441 agcgcgtcgc cctcgtttcc ggcatcggct gctcctcgcg cctgcccgcc tacaccaacg
     7501 tgttcggctt ccacggcgtg catggccgcg ccctgccgat cgccaccggc atcaaggtat
     7561 cccgccccga gctcaccgtg atcgcctccg gcggcgacgg cgacggcttc tcgatcggcg
     7621 gcaaccactt catgcacgcc tgccggcgca acgtcgacat gacctacatc gtcatggaca
     7681 acgaggtcta cggcatgacc aagggccagg cctcgccgac caccgcgccc gactgggaga
     7741 agagcaagct caccccgcag ggcaccggca tcaacccctt ccacccgctg gtgatcgcac
     7801 tcgccgcggg cgcgaacttc atcgcccgcg gcttctccgg cgaccagaac ggcaccgcga
     7861 agctcatcac cgaggcgatc cgccatcccg gcttctcctt cgtgcagctg ctcagcccct
     7921 gcgtcacctt ccgtcccgag cagcgcgact ggaaggacat ggtgcatccc gccgcggtcg
     7981 acccgaccga cgaccccgcg cgcgccgcgc gccgcctgat gtccgacgat ggcttcaacg
     8041 tcggcgtgct cttccgcggc agccgcaagc ccttccagcc cgggcagccg gcgagcgtcg
     8101 attcagttgc agatctcgag aaggagttcg tgttatgagc aaacccgatc cgcaggaaac
     8161 cggcatccac tcggtggagc acctgctggc ccatgcgctg gcgatggaag tcgaggcggt
     8221 cgagcgctac gaggcactcg ccgaccagat ggagacccac aacaaccgcg acgtcgccgt
     8281 gctcttccgc aagctggccg agatcgagaa gctgcacgtc gacaacgtca acgacatcag
     8341 cgagggacac accctgcccc acatcgcacc gtgggaatac tcctgggaag cccccgagag
     8401 cccggaggcg ccgtcggtgt cggcagaagg cctgcactac atgatgcatc cctaccaggc
     8461 gatcgcgatg gcgctcgagg ccgagcgccg cggggtggac ttctacgacc gcctcgtcgc
     8521 ccagtccgag cgcgaggaca tccgcaagct cgccgccgaa ctgtgcgaga ccgagcgcga
     8581 gcacgtcacc ctgctcgaag gctggctcgc ccgcttccag cccccgccca agggctggaa
     8641 cgaggacccc gatccgccct tgctgcaggg ttgaggagca aggggtaagg cgccagacat
     8701 gaggcgccag gagcgatgcg cgcgctggga gaccagcgcg cgcatcggct tttggaggtc
     8761 gggcgaaaga tgcctgcgga gaggatgaag ggaaatcccg agcgaaggat ctcgagccgc
     8821 gcccgtccgc cgtcgaccga cgacactgcg accggatgtg acgaggcgcc aggccgagag
     8881 gtcttcaccc ccccaggcct gacgcctcac atctcacatc tcacgccgtc tgatcgacgc
     8941 tcagctcgta gcccgcctcg cgcaggccct gcacgaccgc gccggcatcc ttggcctcga
     9001 agcggatggt cgcggtgcgc aagccctcgg actgcggcgt gcgcggcgac accaccgaga
     9061 cgatgtagcc gccctgcgca ttgaccgagg cgagcagcgc ggcgagctgg ccggcgcgat
     9121 cctgcaactt cacggtgatc cgcgtcgcac cctcgaccca gcagccggtg atgtcgagga
     9181 agaaatcgag caggtccacg ctggtgacga tgcccaccag gcgcccggcg tcgtccacca
     9241 cgacgaggca gcccagcttc ttgggccgca tcatgcgcgc ggcctcctcg atctgcgtgc
     9301 gtggcgagca gctcaccacc gacttgtgca tgatgtccgc cgcggtgatc ttggacagca
     9361 ggtacttcac ctcgccgacg tcgagcgtcg tgatcatgga cggctgggcg cgctgcacgt
     9421 cgcgatgcga gatgatgccc agcaccttgc gatcctggtc gatcaccggc acgtggcgca
     9481 ccttcttgag ccgcatgatc tcggagacct gcgagaaaga ggcctccgga ccgacgtgca
     9541 gcacctcccg ggtcatgatg ttttcgacga acatgggtca ctccagttgg aagaagaaac
     9601 agcggtaaac agcgggttcg agacagcaca agaggaatag cgggcacgcg acgcggcccc
     9661 ggccggacac ggaccagcgc gcatgcggtc gccggatctg cggctggaac gcagtttatc
     9721 ggaaacccac atccccaagc agcgcctata gttaacgcta acgttaactc tacacaaaaa
     9781 aattacgttc acgtcaacgt tactgtttga caaatatcaa ccgaccggtc agtcataagt
     9841 tctaggattc atcccataag ccaagaaggg ggagcgggcg gcctcagcct cgcccgcaca
     9901 ccaccggagg agacacctga tgaccgtcag cagcccacag ggcacaacca aggtcgccac
     9961 ctactgttac cagtgcgtgg cggggccgga cctgctcaag gtgaaggtcg aggacggcgt
    10021 ggcgaccgcc gtcgagccga acttcgacgc cgaggacgtg catccggccg cgggccgggt
    10081 ctgcgtgaag gccttcggcc tcgtgcagaa gacctacaac ccgaaccgcg tcctgcagcc
    10141 gatgaagcgc accaacccga agaaggggcg ccacgaggac ccgcgtttcg tgcccatctc
    10201 ctgggatgag gcgctcgaca ccatcgccgc caagctgcgc ggcatccgcg agaccggcct
    10261 gctcgacgcc tcgggctacc cgcgcgtggc ggcgagcttc ggcggcggcg gcacgcccac
    10321 cgcctacatg ggcaccttcc ccgccctgct ggcggcctgg ggcccggtgg acatgagctt
    10381 cggcagcggc cagggcgtca agtgctacca ctccgagcac ctctacggcg agctctggca
    10441 tcgcgccttc atcgtctgtc cggacacgcc gcgcagcaag tacatcctgt ccttcggcag
    10501 caacatcgag gcctcgggcg gcgtatgcgg cgtttggcgc cacgccgccg cacgcgtcga
    10561 gcagggcgtc aagcgcgtgc aggtcgagcc gcacctgtcg gtcaccggcg gttgctccgc
    10621 cgagtggctg ccgatcaagc ccaagaccga cgccgcctgc atgcacgcga tgatccacgt
    10681 gatgctgttc gagaacgcac gctcgcggct ggacatcgat ttcctcaagc acatgacggc
    10741 gtcgccctac ctggtggcgc ccaatggctt ctacctgcgc gatcccgaca cccgcaagcc
    10801 gctggtgtgg gacctcaagt ccggcaaggc cgtcgtcttc gacacccccg gcatcgatcc
    10861 cgcgctcgac ggcgacttcc tcgcctccgg cctcgaggtg ctgcccgacc aggaactcgt
    10921 cacccatgag gccgcgccgg tgcgcagtgc cttcggcaag ctgctcgacc acgagcgcac
    10981 cttcaccccg gagtgggcgc agggcatctg cgacgtaccg gcggcgacca tccgccgcat
    11041 cgccaacgaa tacctcgacc atgccgagat cggcgcgacg atcgagatcg agggccgcac
    11101 cctgccctat cgcccggtgg cggtcacgct cggcaagacg gtgaataacg gctggggcgg
    11161 ctacgactgc tgctgggcgc gcacgctgat ggcctgcctg gtcggcgcgc tcgacgtgcc
    11221 cggcggcacc atcggcaccg cggtgcgcct gaaccgccca gccaacgacc gccagagcag
    11281 cgccaagccg ggcgtcgacg ggttcatgga ctatccgttc aacccgaccg acaaggaaaa
    11341 ctggatctcg cgcccgcaga tccgcaacgc caaccgcacc ctggtgccgc tggtggccaa
    11401 ctcggcgtgg agcgccgcgc tcggccccac ccacctggcc tggatgcagc agcgtcacgg
    11461 cttcgagagc ttccccgagc ccacccagcc cgacgtctgg ttcttctacc gcaccaaccc
    11521 ggtgatctcg ttctgggaca cgccccaggt cgccgaggcg gtggcgaagt tccccttcgt
    11581 ggtcgccttc acctacaccc gcgacgagac caaccacttc gccgacatcc tgctgcccga
    11641 ctgcaccgac ctcgaaggcc tgcagatgct gcgcatcggt ggcaccaagt acgtcgagca
    11701 gttctgggac caccagggct tcgcgctgcg ccaaccggtc gtgccgacgc agggcgacac
    11761 ccgcgacttc acctggatca gcaccgaact ggcccgccgc gcgggcatcc tcgagccgta
    11821 caacaaggcg atcaaccgcg gcgccgcagg cgtgccgctg aaggggccga actacgattt
    11881 ctcgctgcag cccgaccacg cctacggggt cgaggagatc tgggacgcga gctgccgcgc
    11941 ggccagcgcc gagctcaccg aaggcgccga ggaccacggg ctggagtggt ggaaggagca
    12001 cggcttccgc accatcgagt atccgcgcct gcagtggtat ctctatcccc acctggtcga
    12061 ccaggggctg cgcttcgaga tgccctacca ggagcgcatc taccgcatcg gcgaggaact
    12121 cggccgccgc ctgcacgaat ccggcatcga ctggtgggac cgccagctca ccgaataccg
    12181 cccgctgccc gagttccacg acttctccca cctcatcaag cacgcggtga tctccaacct
    12241 gggcgggcgc gacgaggact tcccgttctg gctgctaacc gcgcgcagca tgcagtactc
    12301 ctggggcggc aacgtcagcc tgcagatggt gcgcgaggtg gccgccaacg tgaagggcca
    12361 ccgcggcgtg atcatgaacc cgacggccgc acgcaagctc ggcatcgagg acggcgacct
    12421 cgtcgaggtg cgctcgccgc tgcgcgagac ccgcggtcgc gtcgtgctgc gccagggcat
    12481 ccgccccgac accctgctga tggtgggtca gttcgaccac tggatcacgc cctacgcgaa
    12541 ggacttcgac gtccccagca tgaacgccct ggtgccgatg ctgatggacc tgaccgacgc
    12601 caccggttcg gccgccgaca tcgtgccggt gtcgatcaag cgcatcggag gtgcccaatg
    12661 acccgctacg ccatggtggt cgacctgcgc cgctgcgtcg gctgccagac ctgcaccgcc
    12721 gcctgcaagc acaccaacgc caccccgccg ggcgtgcaat ggcgctgggt gctcgacgtc
    12781 gaggcgggcg aattccccga cgtcacccgc agcttcgtcc cggtgggatg ccagcactgc
    12841 gacgagccgc cgtgcgagac ggtgtgcccc accaccgcca ccaagaagcg cgccgacggt
    12901 ctggtcacca tcgactacga cctgtgcatc ggctgtgcct actgttcggt cgcctgtccg
    12961 tacaacgcac gctacaaggt cacccgcgac acccccgcct atggcgaggg caagatggcc
    13021 aacgaggtga agcgtaccga cccggcgcgc atcggcgtgg ccaccaagtg caccttctgc
    13081 tccgaccgca tcgactacgg ccacgcccac ggcctcgtgc ccggcgtcga tcccgacgcc
    13141 accccggcct gcgccaacgc ctgcatcgcc agcgcgctca ccttcggcga catcgacgac
    13201 cccgacagca aggcctcgcg cctgctgcgc gagaacgagc atttccgcat gcacgaggag
    13261 ctcggcaccg gccccggctt cttctatctc tgggagaaaa agcaatgaga ggcgtcgaac
    13321 ccgaactgca gcccttctgg gacgaacgtg ccgcgggcaa cttcatcggc ggcggcaccg
    13381 gcgccggact gctgctgtgg agcgcgatcg cggccagcgg ccacggcacc gcgatcggct
    13441 tgccgctctt cctcgcgctc gccttcatcg gcgtcggcct gttctgcgtg tggatggaga
    13501 tcggtcgtcc gctgcgcgcg ctcaacgtct tcttccacgc gcgcacctcg tggatgaccc
    13561 gcgaggcgat cgtcgccggc ccgctcttcc tgtgcggcct catcgcgctg ctcagcggct
    13621 ggttgttctt cgcctggctc gccgccctga tcggcctcgt cttcctttac tgtcaggcgc
    13681 gcatcctgca ggccgcgcgc ggcatcccgg cctggcgcga gccggcgctg ctgccgctga
    13741 tcgtggtcac cgggctcgcc gaaggcgcgg gcatgctcgc gctgctctcg ccgctgctcg
    13801 tccgctcgcc cggcgccgag ttcgccttcc cgctgctggt gctggtcgtg ctgcgcttct
    13861 ggctgtcgcg gcgctacgcc gcccatctcg cccgcccgaa caacgccccc ctgaagacgc
    13921 tgcgcgcgct gcgcgagaac gatcgccact tcctcctcct cggccacacc ctgccggcgg
    13981 cggtgctgct gatcgcgctc ttcgccggcg gcagcttcgg caccgcactg ctcgcgctcg
    14041 cgggcatcgc ggtcgccgcc agcggctggc tgttcaagct cgcgctcatc acccgcatcg
    14101 cctacaccca gggtttcgcg ctgcagcacg ttcccgcgcg cacccccggc tattcgcacc
    14161 ccggcgtcaa gccgggctgg aactgaaccc ctccctccac cccggggcgg cggaccatgc
    14221 cggaccgccg ccgaaatcag gagcaaagcg catgtacgag gtcaggatcc atggtcgtgg
    14281 cgggcagggc gcggtgcttg cggcccagct gctcgcgacc gcactcgtgt tcgacggcaa
    14341 gtacgccgtc tccattcccg ccttcggctt cgagcgccgc ggcgcgccgg tggtgtcctt
    14401 catccgcgcg agcgaacagc cgatccgcca gctcaccaac atctacgcac ccgactgcat
    14461 cgtctgcgtc gatcccgcgc tcgcccgcac cgtgaacatc ttcgccggcc tcaagcccgg
    14521 cggcaccctg gtgcaggcga cctcgcgccc gctcgccgag atcgacgtgc ccgacagcgt
    14581 cggcaccgtc ggcctgtgcg acgcggtgcg catcgccacc gagatcttcg gccgcccgat
    14641 caccaacacc ctgatgctcg gcgccttcgc gcgcagcacc ggccaggtct cgctcgaggc
    14701 gctgaagaag gtgatcgagc aatccgattt ccgcgacgcc ggcctgcggc agaacctcga
    14761 cgccctcgag cgcggctatg cggaaacgac cgtgcacacc ctcgaacgga ggatcacagc
    14821 atgagcgaac gccaccaaca ctacccggcc tggcacctca aggaggccgg ggttcccgac
    14881 gacctctgtc cggtcgccac cgagcagagt ccgatgttgc cgggcgactg gcgcagcttc
    14941 cgcccggtcg tcgatcgcga caagtgcgtc aagtgcgcgg tgtgctggct ctactgtccg
    15001 gtgcagtgcg tcgaggagca cgccgcctgg ttcgacttcg acatgaagac ctgcaagggc
    15061 tgcggcattt gcgcgcacga atgcccgcag cgggcgatca ccatgattcc ggaggcgcaa
    15121 tgaacaccac cacgctcgac accgctccca ccaccgaaaa gcgcccggca ccgcagctga
    15181 tgctgtgcga gggcaatgag gccgccgcgc tcggcgtcgc gctcgcccgc cccgacatgg
    15241 tcgcggtgta cccgatcacg ccgcaaacct cgctggtcga gaagctcgcc aagctcatcg
    15301 ccgacggcag gatggacgcc gacatcgtcg atgccgaggg cgagcattcg gtgctgtcgg
    15361 tgctgcaggg cggcgcgctc gccggcgcac gcacctacac cgcgacctgc ggccccggcc
    15421 tcgccttcat gttcgagccc tatttccgca cctcgggcat gcgcctgccg ctggtgatga
    15481 ccatcgtcac ccgcgacggc atcaccccgc agtcggtgtg gggtggccac caggacgcga
    15541 tgacggtgcg cgaggcgggc tggatccaga tgtactgcga gaccgtccag gaagtcctcg
    15601 acaccacggt gatggccttc cgcctcgccg agcatcacga cgtgatgctg ccggtgaacg
    15661 tctgcctcga cggcaactac ctctcctatg gcacctcgcg catcgagatg cccgatcagg
    15721 ccgaggtgga cgacttcatg ggacacaagg acgtcaactg gcacgtcgcc ctcgacccgc
    15781 tgcgtccgat ggcggtcgac ccgctcaccg gcggctcggg cggcaacggt ccggagacct
    15841 tcgtgcgcta ccggcgcggc cagtgcgccg gcatgaagaa cgcactgcac gtgatcaccg
    15901 agatgcacga ggactgggcg cgccgcttcg gcgaagccca ccgcttcgca ccgctggtcg
    15961 aggaataccg gctcgacgac gccgagtacg caatcatgac cctgggcagc atgaccggcg
    16021 ccgccaagga cgcggtcgac gaggcccgcg cggccggcga gaaggtgggc ctcatcaaga
    16081 tcaagacctt cagccccttc ccggtcgagg cgctgcagca cgcgttgcgc ggcgtgcgtg
    16141 cgctcggcgt ggtcgaccgc tcggtcaatt tccgctggaa ctgcggcccg atgttccagg
    16201 aaaccctcgg cgtgctctac cgcctcggcc gccaggtgcc ggcgcaaagc ttcatcggcg
    16261 gcctggccgg cgccgacctc accgtcacca ccttccgccg cgtcatcgac gagacccgcg
    16321 ccctgctcga gcgagcgccc tccgacgagc cggtgtggct caacgccaaa gactgagcgc
    16381 caaggacaga ggaaaccacc atgcacacca ccgactacct gatcgccggc agcagccacg
    16441 ccgcgctcga ggcgatcaac gcgatccgca tgcacgacgg cgagggcagc atcaccgtcg
    16501 tcacccgcga cccgcaccgc ccctactcgc ccaccgtgct gccctacgtg gtctcggggc
    16561 gctcgacgcc cgaacgcgtg ttcctgcgcg acgacgactt cttcaccgcg aacaaggtcg
    16621 attaccgcag cggctgcgcg ctcgccggcc tcgaccccgc ggcgcatcag gcgcgcctgg
    16681 ccgacggcag caccatcggc taccgcaagc tgctcctcgc caccggcgcc agcccgatcg
    16741 tgccgcccat ccccggcatc gagcgcgtcg actaccacgt gctgcgcacg ctcgacgacg
    16801 ccacccggct gcgtgcggcg atggccgcgt cgaagaacgc ggtcgtgctc ggtgccggcc
    16861 tggtcggcat gcacgccgcc gaaaacctgg tcaaggccgg ggccagcgtc accatcgtcg
    16921 agatgagcaa gcagctcacc tccggctact tcgacgaggt cgccgccggc atgatcgagc
    16981 aggccttcct cggcaacggc gcgaagatcc gcaccggcca ccgcgtcgtc ggcctcgagc
    17041 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgccgctca acacctacca
    17161 cttcttcggc cgccacgcga tctcggtggg cagcgccgcg gtccccgagg gcgggcgcgc
    17221 gcaggtgcgc ttcgacgccg cgagcgggcg ctacctgcgc gcggtgttcg accgcgacga
    17281 gcgcctgacc ggcatcttcg gggtgaatga attcttcgat gccggcgtga tggcccagct
    17341 catcctgcgc cgcaccgatc tcggaccgct gcgcgaacgc ttcttcgcgc agccgctcgc
    17401 caccggccgc gagctcatgt cgcagctctg gcgctaaggg gaagatcatg ggacaagcct
    17461 actccaccat cgccttcgac cccgccaagt gcgacggctg cggcaaatgc atgacggcgt
    17521 gcgcgagcgt caagttcgac tccaccgaca ccacgcgctc gctgatccag atcgtgcgca
    17581 gcggcggcgc ccgcatcgag ccgccccgcc cgggcgagtt cgagctcgcg ctgtgccgcc
    17641 agtgcgccga tcccaagtgc gtcaccgtgt gcccggcggg cgcgctcggc aagaacggcg
    17701 ccaccggcgt gatcgactgg gacgcctcga agtgcgtcga ctgcctgctg tgcaccatcg
    17761 gctgcaccta cgccggcatc gcgctcgacg agcacagcgg ccgtgtcagc aagtgcgaca
    17821 cctgcgaggg caagccggcc tgcgtgcccg cctgccccac cggcgcgctg cgccacatca
    17881 ccaccgcgcg catctacaac gaggtcggca gctgggaaga cctgttcgca cccggcctgg
    17941 ccggctgcca gggctgcaac accgagctgc tgatgcgcca cgcgctgcgc cgcgtcggcc
    18001 ccgacaccgt gctcgccacc ccgccgggct gcgtgcccgg catgggctcg gtgggcttca
    18061 acggcaccac cggcaccaag gtcccggtct tccacccgct gctcaccaac accgcgtcga
    18121 tgctcgccgg ggtcaagcgc cagtacgagc gcgtcggccg caaggtcacc gcgatggcga
    18181 tcgccggcga cggcggcgcc tcggacgtcg gcttccagtc gctctcgggc gcggccgagc
    18241 gcggcgagcg catcctcttc atggtggtcg acaacgaggg ctacatgaac accggcatgc
    18301 agcgctcgag ctgcacgccc tacggcgcat ggacctcgac caccccgatc ggcgcacatg
    18361 gccgcggcaa gacgcaagac gccaagaacc tgccgatgat catggtgaac caccgctgcg
    18421 aatacgtcgc caccgcgtcg accgcgtaca tggaggacct ctacgagaag ctcgacaagg
    18481 ccctcgccgc ctccgagcgg ggcttcgcct acctgcacgt gtattcgccc tgcaccaccg
    18541 gctggcgctt ccagagcgcg ctcaacatcg aggtcgcgcg caaggcggtg gagtcgaact
    18601 tcgtcaccct gtgggaatac acccccgagg ccgggctgca cttcacccgc ccggtggaca
    18661 agccgctgcc ggtggccgac tacctcaagg ccatggggcg cttccgccac ctcagcccgg
    18721 agcaggtcga gcacatccag aacaaggtgg ccgagaacct gcgcttcgtg cgcaagatcg
    18781 cggagcaggc cgatgagcaa tgagaagccg tcccccccgg agcagatgag ccgcgccgag
    18841 ctgcaggcgc tgcagctcga gcgcatgcgc tggtcgctgc agcacgcctg ggacaacgtg
    18901 ccgcactacc ggcacgcctt cgaggccgcc ggcgtgcggc catccgacct gcgcggcctg
    18961 gccgacctgg cgaggttccc cttcaccgcc aagcaggacc tgc
//
LOCUS       DAOQNN010000018        18556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1110_length_18556_cov_12.546781, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000018 DAOQNN010000000
VERSION     DAOQNN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 18556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18556
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1110_length_18556_cov_12.546781"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKV97_02390"
     CDS             <1..308
                     /locus_tag="PKV97_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB04753.1"
                     /translation="AAPAAAPVAAAPAAAPAGAAGEGTYKKVCALCHASGVAGAPIPG
                     NKADWEPRVAQGKDTLYKHAIEGFTGSKGMMPPRGGGASLTDDEVKAAVDFMVGKLK"
     gene            complement(396..1028)
                     /locus_tag="PKV97_02395"
     CDS             complement(396..1028)
                     /locus_tag="PKV97_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNB04754.1"
                     /translation="MSIVRGRRRLLLAGAAVSLAAGSALLWDYTTRAGRSFEPPAEDI
                     CVVTPTRAAQAHPYDPASGLGKYDARPIPADARCPVCGMYPARFPRWAAQIVFEDGAA
                     HFFDSPVDLFMFLDKPASFGSTRTAAEAAAMYVADFRNGTWLPARQAVFVRDSRARGP
                     MRGPDLPAFADTAAAQAFIAEQGGRALTFEQVDASVVTGLRDANHAHHMH"
     gene            complement(1025..1399)
                     /locus_tag="PKV97_02400"
     CDS             complement(1025..1399)
                     /locus_tag="PKV97_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04755.1"
                     /translation="MSLHTELLDYVERAHEGALLAPARLSQDALNLVLRNGVVLTVRY
                     AAPDAYSLRWRTSADIDGVELGIDTAPVHPQLETTPNHLHRADGSVAADPLTDPRRSP
                     QENLARVIDVLGRDPQLADFGT"
     gene            complement(1399..1668)
                     /locus_tag="PKV97_02405"
     CDS             complement(1399..1668)
                     /locus_tag="PKV97_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04756.1"
                     /translation="MNPIFDAIPPEQALELEQIARLIYEARENRRKVLAAIGANDEAE
                     LLARIAAGAVDEHPGYEHYLAARILADTHEAARQVVGERLREINR"
     gene            complement(1686..2231)
                     /locus_tag="PKV97_02410"
     CDS             complement(1686..2231)
                     /locus_tag="PKV97_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNB04757.1"
                     /translation="MCTHHACPSRRRFLIAGVAAGLLAACGSGKQGAGSGTVTPLEID
                     RATSCALDGMLLADYPGSKVQMHYAGVERPDWFCDTIEMFNVYLNPEQARLVTAIFVQ
                     DMAKASWNAPQGHWIDAKKAWYVFGSRRMGAMGPTAASFANEADAKAFAAENGGKVLR
                     FDEVTPDMAILDGGALHDQSM"
     gene            complement(2270..3085)
                     /locus_tag="PKV97_02415"
     CDS             complement(2270..3085)
                     /locus_tag="PKV97_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB04758.1"
                     /translation="MEFSQIATIAGKEFWDRIRNRWVLAVALVFTVFALSIAYFGSAQ
                     QGAVGFRSIEVTIASLVSLVIYLIPLIALVLGFDAIVGERERGSLDLLLSMPITRLEL
                     LLGKYLGLAGALTFSTVAGFGLVGVVLSAQLDLGSLFHYFGFMLSSVLLGCAFLSIAV
                     MLSVFAADRTRASGLAIATWFFFVLIFDLLVLGVLVVTAGRWGGEALPYALLFNPADV
                     FRILNIFSMEDVRTLYGLSTVFPPALARPWLLGLVMAAWIAGPLAIATWRFRR"
     gene            complement(3096..4037)
                     /locus_tag="PKV97_02420"
     CDS             complement(3096..4037)
                     /locus_tag="PKV97_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB04759.1"
                     /translation="MADISPAQNDPGAPVIVARGVRKHYGPIHAVDGVDLEVRSGELF
                     GLIGHNGAGKSTMFKMMLGLIPLTAGEIRIDGAPVPGSDFRTVRRKIGYLPENVVLYD
                     NLTGTETLDFFARLKGVSTANNAAVLERVGLMHAAKRRVREYSKGMRQRLGFAQALLG
                     KPRILFLDEPTTGLDPEAIRGFYAILRQLKSEGVTMVITSHILAEIQERVDRLAIMAA
                     GKVQATGTVQALREQMDLPLWFDVRVAPEDFEAVRNALGGLPVGAIEARDGHVAVQCA
                     RDAKMAVIAALATLNGKVRDLTVREPSLEDVFFGFSD"
     gene            complement(4009..5295)
                     /locus_tag="PKV97_02425"
     CDS             complement(4009..5295)
                     /locus_tag="PKV97_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HNB04760.1"
                     /translation="MRSLHRLPRLLAAAALLAALATPAAATPWRVTPGESIQAAIDRA
                     APGDVIEIERGRYAGQLLVDKPLTLRGIERPTIDGELKENTISIKAESVTIEGLIVAN
                     SGGDTLYQHAGVYIYPGSHKAVVRNCDFSYTLFGLWIEKANDVLIENNLITGKRDFDS
                     PKRGNGIQLYNTQRARIIGNNISFVRDAIYVDVSHDALFRGNKLHHSRYGTHYMTSYR
                     NVWEDNETWYNRGGLALMEVRDQVVRNNRAWGNSDHGIMLRSIQDAVIENNVVAGNQR
                     GFFIYDAEYNELRGNQIIDNVVGIHLWAGSKNNKVERNDFIGNREQIRYVAAHDMIWG
                     EKEGNHWSNYLGWDRNGDGLGDVRYEANDLVDRLTWRHPMMKLLLGSPAIQTLRMVAQ
                     QFPLLRAPSIVDPRPRMEPNKPDWSQWRGRHFPRSE"
     gene            complement(5448..5873)
                     /locus_tag="PKV97_02430"
     CDS             complement(5448..5873)
                     /locus_tag="PKV97_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription regulator"
                     /protein_id="HNB04761.1"
                     /translation="MVDPRILTEQVEPPYASRGSASRLPAEIWDHLWPWSRNGFQRQR
                     VVQAAGLALAAAASVAWILAAMGNLTPGAIIGWWFGWSVFEVAVRLGSKPYVKDGPWW
                     GSRYRRASIMDMICYVGFKNLLIGAALFIVLKSMGLVQV"
     gene            complement(5905..8088)
                     /locus_tag="PKV97_02435"
     CDS             complement(5905..8088)
                     /locus_tag="PKV97_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB04762.1"
                     /translation="MKPLSALRGIAMAALLAVTTLLPQSASAESAYEAKLPPEVNTAP
                     ALCDYVPCKEVLPGATSFSERKGQPPYVEGYAEENGQRKLVGYVVLSTDVTDTPAYSG
                     KPVITLIGLTPQGNFAGIKILKHSEPILLLGIPESALVKFNNQYLGKFVGANIEIGRS
                     RPEEEIIGLDAISGATVTVIAQNQVMMMSGAAVGRQVGILEKTVRPQARFVDSGRKLD
                     WATLVKEGVVQRLTVMPEQVGLERGKDPFIDLWYGYLNHPEVGRAIMGETGWRDLMAR
                     IKEGEHALFVIRTAGRESFKGSGFVRGGIYDRVQLRQGQDVFTFRDLDYYNLYGISAP
                     GAPSFTESAIFIVRSSSFSGAFPWKFIFLGNRIDRETGARTFINFDREYWLPDAYIEG
                     GRPEVKKPDAPWVRVWKTRALEIVLFSALLVAVAVVYAKRDELTRRSTRKNKWPVNAF
                     KYSAWIISIGFVGFGLLAQPSITQVLTWFHSLLFKWEWELFLTDPFIFLFWIFIIVTV
                     FVWGRGLFCGWLCPFGSLSELLYKVGGAIGLKRFQFALPMKWHNRLKWVKYAVFFGLL
                     AVSMFSMGLAEKLAEVEPFKTTFLVGMFNRSWPYTLFAAGLLGLSIFMERPFCKYLCP
                     LGASLAMPSTFRWFGLRRKQECDSCKACAVGCGSLAIDEKGRIDHRECMLCLDCMVLY
                     TDDHACPPLAQERKRRTKDGLPLTPIGKDGYYIPIKPVAPAAPRA"
     gene            complement(8276..10234)
                     /gene="nosZ"
                     /locus_tag="PKV97_02440"
     CDS             complement(8276..10234)
                     /gene="nosZ"
                     /locus_tag="PKV97_02440"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
                     /protein_id="HNB04763.1"
                     /translation="MNDAKMNPVEAMPDPSRRKFLNTAALAGLAGAGMSVGLSACNKE
                     TAPAPAAAPATAPAAAPAAAHAASDLKLHMAPGELDTYYGVWSGGHSGECRVLGLPSG
                     REIKRIPTFNIDCMSGWGITNESKKVIGTRPDGQLKYKTGDTHHVHGSYTDGTYDGKY
                     FWVNDKIHGRLGRIRGDIFECDAITEIPNIQGFHGIFSDKRDPVDQAINHTTRVFCGN
                     EFHIPQPNDGRDLDDPTKYYCLFTCVDAETMEVRWQCKVDGNMDLVATSYDGRFACSN
                     QYNTEGGARYEDMMSAEMDACVFFDVARIEKAIKDGKSFTVGSSKVPVVDGTKAANPD
                     PKTALTAYVPVPKNPHGVNASPDGKYMICSGKLSPTCTVIEVAKVADFLDGKLDDIRK
                     AVVAEVEVGLGPLHTTYDGRGNAFTTLFLDSQIVKWNIAKAIQFHNGDKAAQYVVDRI
                     DVHYQPGHISANMGETKEASGQFLAVGCKFSKDRFLPVGPMHPENEQLIDIRGEKMVL
                     LADHPVHPEPHDFIIVNRDIIKTRQVGVLEAHPLAIKDAKESGVTRDGNKVTVKIASQ
                     APAYSLREFELKVGDEVTLILTNLDKVEDLSHGFAIPKYDINFVVNPLETKSVTFKAD
                     KPGVFWCYCTHFCHALHLEMRTRMLVRP"
     gene            10447..11775
                     /locus_tag="PKV97_02445"
     CDS             10447..11775
                     /locus_tag="PKV97_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB04764.1"
                     /translation="MQPIEQETEPAEDLDNPNPVLMPARVLNAVRTDRGRLLQLRWVS
                     LLAMVLMSLVVFPWLAPAQPVGPLAGVTLCLLAVNLALLGGAARWLVGRWGAFLQLAV
                     DLLAWAAFLYFTGGVTNPAISLLLPVVAVGASILPAAQAWALAALAVALYSVLWQFHQ
                     PVRLADADQAMYWHLAGMWISFAVSAATVVWFIVRLNVELARRDNELAAVNAARARDT
                     YAVGLGKLAAGAAHRLGTPLGTLRILADDLASRPELASGLREDVQLMREQVDYCRDIL
                     NGLTREAGQQRAESGGAVEVRAWVTEVGGRWQRLRPGAGLHIDAEAADGQAVIVADAS
                     LGDALHNLIDNAANANERAGARARAVELGIRREGARVVVEVLDRGPGLPPDRVEAARL
                     ARLGERAQGMGVGLMLAHAAVEHHGGHLEFRSRPGGGTIARMLIPRQSVR"
     gene            11772..12314
                     /locus_tag="PKV97_02450"
     CDS             11772..12314
                     /locus_tag="PKV97_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB04765.1"
                     /translation="MTPDNTPCLLVVDDDPAFNRVLVRALGQRGFDTYGATDSEGALE
                     LARDHEPEFIVLDLNIGGESGLKLIRPLLEASPGARILVLTGYASIATAVDAVKLGAV
                     QYLAKPADIDSIIRALRAEGVRPEDHAPGAPLSVDRLEWEHIQRVLAEHHGNISATAR
                     ALQMHRRTLQRKLARPPAGA"
     gene            12418..12936
                     /locus_tag="PKV97_02455"
     CDS             12418..12936
                     /locus_tag="PKV97_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HNB04766.1"
                     /translation="MRRLLLLLTPVLAATAHASPVPAEAPIRMAGLWLMNTTPVGAAA
                     QTSSFHICIGAPARDDVLAHPGSAVQNCREERWSKDEHYTYYQASCEAKGGNAAVEAR
                     FAGDFKYNFQGEVTMTIASQAGAAEVTRMEIDGRRLAPCRGGLPEGRFLIKGQDGVGN
                     LNLGEPIRQPAR"
     gene            13056..14231
                     /locus_tag="PKV97_02460"
     CDS             13056..14231
                     /locus_tag="PKV97_02460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HNB04767.1"
                     /translation="MPPRRQHYRGRNPARAHSIAELRAMAARRLPDFCLEYLDGGADD
                     ELALARNRRVLDEILLLPRTLVDVSQRELAVPLFGTDIALPAVIAPTGFNGLLTHAGD
                     RVLAEAAHAAGIPFCQSMVSTVALEDIAATGVRHWMQIYPFKDRDNLAAVVKRAEHAG
                     CEAIVLTTDASVFGNREWDRRNYRAPMKLAWRKLLDVGLHPRWVLDVLVPHGMPRFRN
                     LGDFLPPGMDSAKNAAAFLAAQMDTSLTWEDLRRLRDLWPRRLLVKGVLLPEDALRAQ
                     EAGADGVVVSNHGGRQLDCAPAPIETLAAVRQAVGAEMTVIVDSGFRRGSDFVKARAL
                     GADAAMSGRATLYGLAAAGAAGAARALEILRGEMERTLGLIGCARMDEVDARYVGRG"
     gene            complement(14256..14603)
                     /locus_tag="PKV97_02465"
     CDS             complement(14256..14603)
                     /locus_tag="PKV97_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HNB04768.1"
                     /translation="MLVTFKSHASADVIMFGDAAKKLIGILGKDPADAKGIVTVQQLP
                     EAIARLKAAIEEERARQAELERQRSHADEEAEKEAGNTGMAAPVGLAQRAWPLLAMLE
                     ESLREETPVVWGV"
     gene            complement(14622..15179)
                     /locus_tag="PKV97_02470"
     CDS             complement(14622..15179)
                     /locus_tag="PKV97_02470"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HNB04769.1"
                     /translation="MSLNLEEIKRAREEADCLADAATVEAALDRMAAEISARLADANP
                     LVYAVMNGGLILAGRILPRLPFPLEVAYLHATRYGHAIQGTLLDWRVRPTQDLRGRTV
                     LVLDDILDEGHTLEAIIAHLKEEGAAEVLSAVLVHKLHERKARPGMRADFTGLDIPDR
                     FLFGCGMDYKGYWRNAPGIYAVKGL"
     gene            15379..16011
                     /gene="upp"
                     /locus_tag="PKV97_02475"
     CDS             15379..16011
                     /gene="upp"
                     /locus_tag="PKV97_02475"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNB04770.1"
                     /translation="MPIHEIRHPLVRHKIGLMREGDISTKKFRELTAEIARLLAYEAC
                     QDFPLETVEIRGWAGPVEIEQIKGKKVTVVPILRAGLGMLDGVLDMVPSAKVSVVGIA
                     RDEETLQPVPYFEKFVGQLDERMALIIDPMLATGGSMVATVDMLKRKGCTHVRALVLV
                     AAPEGIALMDEKHPDVDIYTASIDSHLNEHGYIIPGLGDAGDKIFGTRHN"
     gene            16124..17371
                     /locus_tag="PKV97_02480"
     CDS             16124..17371
                     /locus_tag="PKV97_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HNB04771.1"
                     /translation="MREIPIQSAEPLWRQAISGAQILFVAFGALVLVPLLTGLNPSLA
                     LLGAGVGTLLFQLLTGRQVPIFLGSSFAFIAPIIFSIQTWGIPATMGALACVSLMYFV
                     FAGLVWKHGAEIVHRYMPPVVVGPIIMIIGLSLAAVGVNMAMGRSGDGKLELVPYATA
                     LMVAGISFGTTVLVAVFARGFLKLVPILCGVAAGYVAAILFGLVDFAAVGAAPWFVLP
                     QFVTPSFELAAILFMLPVALAPAIEHVGDVLAIGGVTGKDYTEKPGLHRTLAGDGAGV
                     LFAGLVGGPPITTYSEVTGALTLTKNYNPAIMTWAAVFAVILAFFGKFNALLASIPVP
                     VMGGIMMLMFGSVAAVGLNTLIKARVDLEEPRNLVIVSSVLVMGIGGLALNFDGLTLQ
                     GVSLCAVVAVVLNLILPNARAGA"
     gene            complement(17304..17705)
                     /locus_tag="PKV97_02485"
     CDS             complement(17304..17705)
                     /locus_tag="PKV97_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HNB04772.1"
                     /translation="MDPRQVELALRKQKLQLRAEAQRADITQRLAGIDGALDRVDALR
                     DHLAWAKDKVPVLSVALLVVLAARPRLTLRLAKRAWVGWLLLRQARGGRLSALLPTAV
                     PLLRGLLALLRRRLERSAGSGSAPPRRRRTS"
     gene            complement(17719..18111)
                     /locus_tag="PKV97_02490"
     CDS             complement(17719..18111)
                     /locus_tag="PKV97_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNB04773.1"
                     /translation="MSTEPRPPRPRLAESLHGIVDAGLQTAQTRLELLAVELQEEKLR
                     LTGLALNTVLAGLLLGFGLVFLMVFLTVLFWDEHRLLALGISTAICLGGGLLAASNAA
                     RAFRSGTKLFSASLAELARDRAALKKPD"
     gene            complement(18116..18427)
                     /locus_tag="PKV97_02495"
     CDS             complement(18116..18427)
                     /locus_tag="PKV97_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HNB04774.1"
                     /translation="MATTLQPSKDKIVDDLKSLISDAEELLKLTADQQGGKLDDLRAR
                     INGRVAVAKDRLADAEAAIVDSGKKAVRATDDYVHENPWQSVGVAAGVAFLLGLLVSR
                     R"
BASE COUNT         2847 a         5979 c         6586 g         3144 t
ORIGIN      
        1 ccgcagctcc cgctgccgcc cctgttgcgg ccgcgcccgc cgccgctccg gccggtgccg
       61 cgggcgaggg cacctacaag aaggtttgtg cgctttgcca cgcatccggt gttgccgggg
      121 ctccgatccc gggcaacaag gccgactggg agccgcgcgt cgcacagggc aaggacacgc
      181 tctacaagca cgcgatcgag ggcttcactg gctcgaaggg catgatgccc ccgcgcggtg
      241 gcggcgcttc cttgaccgac gatgaagtca aggctgcggt cgacttcatg gttggcaagt
      301 tgaagtaagc cggactgcgg cagtccgctg cggcggacga cgatgtgttt ttcgcggggc
      361 ctgcgggccc cgctttccat cgcgcgccgg tgcgctcagt gcatgtgatg ggcgtggttg
      421 gcgtcgcgca ggccggtgac gacgctggcg tccacctgct cgaaggtcag tgcgcggccg
      481 ccttgctcgg cgatgaaggc ctgcgctgcg gcggtgtcgg cgaaggccgg cagatcgggg
      541 ccgcgcatcg gcccgcgcgc gcgcgagtcg cgcacgaaga cggcctggcg agcgggaagc
      601 caggtgccgt tgcggaagtc ggcgacgtac atcgccgcgg cctcggcggc ggtgcgcgtg
      661 ctgccgaagc ttgccggttt gtccaggaac atgaacaggt cgaccggcga gtcgaagaag
      721 tgcgccgcgc cgtcctcgaa gacgatctgc gccgcccagc gcgggaagcg tgccggatac
      781 atgccgcata cggggcagcg tgcgtccgcg gggatgggtc tggcgtcgta tttgcccagg
      841 ccgctcgcag ggtcgtacgg atgggcctgc gccgcccgcg tcggggtgac gacgcagatg
      901 tcttccgcgg gcggctcgaa gctgcggccg gcgcgcgtcg tgtagtccca cagcagcgcg
      961 ctgcccgcgg cgagcgagac ggcggcgccg gcgagcagga gccggcggcg accgcgtacg
     1021 atgctcacgt gccgaaatcc gcgagttgcg gatcgcgacc gaggacgtcg atcacccgcg
     1081 cgaggttttc ctgcggactg cgccgcggat cggtgagcgg atcggccgcg acgctgccgt
     1141 cggcccgatg cagatggttg ggggtggtct cgagttgcgg atgaacgggc gcggtgtcga
     1201 tgcccagttc gactccgtcg atgtccgcgc tcgtgcgcca gcgcagcgag taggcatcgg
     1261 gtgcggcgta gcgcacggtg aggaccactc cattcctgag caccaggttc aatgcatcct
     1321 gcgacaaccg tgcgggagca agcagtgcac cttcgtgtgc gcgctcgacg tagtcgagca
     1381 gttcggtgtg caggctcatc agcggttgat ctcccgcagg cgctcgccca cgacctggcg
     1441 tgcggcctcg tgggtatcgg ccaggatgcg cgcggcgagg tagtgttcgt agccgggatg
     1501 ttcgtcgacc gcgccggcgg cgatgcgggc gagcaactcg gcttcgtcgt tcgcgccgat
     1561 ggcggcgagg accttgcggc ggttttcgcg cgcctcgtag atgagccggg cgatctgctc
     1621 gagctcgagg gcctgctcgg gggggatggc gtcgaagatc ggattcatcg tggtgcctgt
     1681 cctgcttaca tggactgatc gtggagggcg ccgccgtcga ggatcgccat gtcgggcgtg
     1741 acctcgtcga agcgcagcac cttgccgccg ttttccgcgg cgaaggcctt ggcgtcggct
     1801 tcgttggcga acgaggccgc ggtggggccc atcgcaccca tgcgccggga gccgaacacg
     1861 taccaggcct tcttcgcatc gatccagtgc ccctgggggg cgttccacga ggccttggcc
     1921 atgtcctgca cgaagatcgc ggtgaccagg cgggcctgct ccgggttcag gtatacgttg
     1981 aacatctcga tggtgtcgca gaaccagtcg gggcgctcca cgccggcgta gtgcatctgg
     2041 accttggagc cggggtagtc ggccagcagc atgccgtcga gcgcgcaact ggtggcgcgg
     2101 tcgatctcga gcggcgtcac ggtgccgctg ccggctccct gcttgccgct gccgcaggcc
     2161 gcgagcagcc cggcggcaac accggcgatc aggaagcggc gacggctcgg gcaggcgtga
     2221 tgtgtgcaca tggggaaatc actcgatacg gttcgtttgg aaggagtgtt cagcggcgga
     2281 agcgccacgt cgcgatcgcc agcggacccg cgatccatgc cgccatgacc agcccgagca
     2341 gccagggccg cgccagcgcg ggcgggaaaa cggtcgacag gccgtagagc gtgcggacgt
     2401 cctccatcga gaagatgttc aggatgcgga agacgtcggc cgggttgaac agcaaggcgt
     2461 agggcagtgc ttcgccgccc caccggccgg cggtgacgac cagcacgccg agcaccagga
     2521 ggtcgaagat cagcacgaag aagaaccagg tggcgatcgc caggccggag gcgcgggtac
     2581 ggtcggcggc gaataccgaa agcatcaccg cgatgctcag gaaggcgcag ccgagcagaa
     2641 ccgagctgag catgaagccg aagtaatgga acagcgagcc gaggtcgagc tgcgccgaca
     2701 gcaccacgcc gacgaggccg aagccggcga cggtggaaaa ggtgagcgcg cccgcgaggc
     2761 cgaggtattt gccgagcagg agttcgaggc gggtgatcgg catcgacagc agcaggtcga
     2821 gcgagccgcg ttcgcgctcg ccgacgatgg cgtcgaagcc gagcaccagc gcgatcaggg
     2881 gaatcaggta gatgaccagg ctgaccaggc tggcgatggt gacctcgatc gaacggaagc
     2941 ccacggcgcc ctgttgcgcc gagccgaagt aggcgatcga cagcgcgaac acggtgaaca
     3001 ccagcgccac ggccaggacc cagcggttgc ggatgcggtc ccagaactcc ttgccggcga
     3061 tggtggcgat ctgtgaaaac tccatggtcc gcctctcagt ccgaaaagcc gaagaacacg
     3121 tcttcgagcg aaggttcgcg caccgtgagg tcgcggacct tgccattcag ggtggccaag
     3181 gcggcgatca ccgccatctt ggcatcacgc gcgcactgca ccgcgacatg gccgtcgcgg
     3241 gcttcgatgg cgcctaccgg caggccgccg agggcgttgc gcaccgcctc gaaatcctcg
     3301 ggcgcgacgc gcacgtcgaa ccacagcggc agatccatct gctcgcgcag ggcctgcacc
     3361 gtgccggtgg cctgcacctt gcccgcggcc atgatcgcaa ggcggtccac gcgttcctgg
     3421 atctcggcga ggatgtgcga ggtgatcacc atggtgactc cctcgctctt caattgccgc
     3481 aggatggcgt agaagccgcg gatcgcctcc gggtccagac cggtcgtcgg ctcgtcgagg
     3541 aacaggatgc gcggcttgcc gaggagggcc tgggcgaagc cgaggcgctg gcgcatgccc
     3601 ttggagtatt cgcgcacgcg acgctttgcc gcgtgcatca ggccgacacg ctcgagcact
     3661 gcggcgttgt tcgccgtcga gacgcccttg agacgggcga agaagtcgag cgtttcggtg
     3721 ccggtgaggt tgtcgtagag cacgacgttc tcgggcaggt agccgatctt gcgtcgcacc
     3781 gtgcggaagt cgctgcccgg caccggcgcg ccgtcgatgc ggatctcgcc ggcggtgagc
     3841 gggatcagtc ccagcatcat cttgaacatg gtgctcttgc ccgcgccgtt gtggccgatg
     3901 aggccgaaca gctcgccgct gcggacctcc aggtccacgc catccaccgc gtggatgggg
     3961 ccgtagtgct tgcgaacgcc gcgggcgacg atcaccggtg cgccgggatc attctgagcg
     4021 ggggaaatgt ctgccacgcc attggctcca gtcaggtttg ttcggttcca tgcgcggcct
     4081 ggggtccacg atgctgggcg cacgcagcag gggaaactgc tgggccacca tgcgcaaggt
     4141 ctggatggct gggctgccga gcagcagctt catcatcggg tgacgccagg tcaggcggtc
     4201 gaccaggtcg ttggcctcgt accgcacgtc gcccaggccg tcgccgttgc ggtcccagcc
     4261 gaggtagttg ctccagtggt tgccttcctt ctcgccccag atcatgtcgt gcgcggccac
     4321 gtagcggatc tgctcgcggt tgccgatgaa gtcgttgcgc tccaccttgt tgttcttgga
     4381 ccccgcccac aggtggatcc cgaccacgtt gtcgatgatc tggttgccgc gcagctcgtt
     4441 gtactcggca tcgtagatga agaagccgcg ctggttgccg gcgaccacgt tgttctcgat
     4501 cacggcgtcc tggatcgagc gcagcatgat gccgtggtcc gagttgcccc aggcgcggtt
     4561 gttgcgcacc acctggtcgc gcacttccat cagcgcgaga ccgccgcggt tgtaccaggt
     4621 ctcgttgtcc tcccagacgt tgcggtagga ggtcatgtag tgcgtgccgt agcggctgtg
     4681 atgcagcttg ttgccgcgga agagcgcgtc gtgggagacg tccacgtaga tcgcgtcgcg
     4741 cacgaagctg atgttgttgc cgatgatgcg cgcgcgctgg gtgttgtaga gctggatgcc
     4801 gttgccgcgc ttaggcgagt cgaagtcgcg cttgccggtg atgaggttgt tctcgatcag
     4861 gacgtcgttg gccttctcga tccacagtcc gaacagggtg taggagaagt cgcaattgcg
     4921 tacgactgcc ttgtgcgagc cggggtagat atagacgccg gcgtgctggt agagggtgtc
     4981 gccgcccgag ttggcgacga tgagcccctc gatggtgacg ctctccgcct ttatgctgat
     5041 ggtgttttcc ttcagctcgc cgtcgatcgt ggggcgctcg atgccgcgca gggtcagcgg
     5101 cttgtcgacc agcaactggc cggcgtagcg tccgcgctcg atttcgatca cgtcgcccgg
     5161 cgctgcgcgg tcgatcgctg cctggatgga ctcgcccggc gttacccgcc acggcgtggc
     5221 tgcagccggc gtggccagtg cggcgagcag ggctgcggca gccaggaggc gaggaagacg
     5281 gtggagggag cgcattgcgg gggggcgttt gacagtgggg cagagtatgc gacgcaatgt
     5341 cgcagcgaga cttgatctag gtgaagtgcc cggcggcggc gggcccgcgg aaaacggcgc
     5401 gccgccccgg aggcggcgcg caatgtaacc cgggcgggcc ccggatgtca gacctgcacc
     5461 aggcccatgc tcttcagcac gatgaacagg gcggcgccga tcagcaggtt cttgaagccc
     5521 acgtagcaga tcatgtccat gatgctggcg cggcggtagc ggctgcccca ccacggaccg
     5581 tccttcacgt agggcttgga acccaggcgc accgccacct cgaacacgct ccagccgaac
     5641 caccagccga tgatggcacc gggcgtcagg ttgcccatcg ctgccaggat ccacgccacg
     5701 ctcgcggccg cggcgagcgc caggccggcg gcctggacga cgcgctggcg ctggaagccg
     5761 ttgcggctcc acggccacag gtgatcccag atctcggcag gcaggcggct ggcggagccg
     5821 cggctggcat agggcggctc cacctgctcg gtcagaatgc gcggatcgac catgatcgaa
     5881 tcctcctcga atgcagtgtc ttgatcaggc ccgcggcgct gcgggcgcca ccggcttgat
     5941 cgggatgtag taaccgtcct tgccgatcgg ggtcagcggc aggccgtcct tggtgcgacg
     6001 cttgcgttcc tgtgccagcg gcgggcaggc gtggtcgtcg gtgtagagga ccatgcagtc
     6061 caggcagagc atgcattcgc gatggtcgat gcgacctttc tcgtcgatcg ccagcgagcc
     6121 gcagccgacc gcgcaggcct tgcagctgtc gcattcctgc ttgcggcgca ggccgaacca
     6181 gcggaaggtc gagggcatgg ccagcgaggc gccgagcggg cacaggtact tgcagaacgg
     6241 ccgctccatg aagatcgaca ggccgagcag cccggcggcg aacagcgtat acggccagct
     6301 gcggttgaac attccgacca ggaaggtggt cttgaagggt tcgacctcgg cgagcttctc
     6361 ggccaggccc atcgagaaca tcgacaccgc gagcaggccg aagaacaccg catacttcac
     6421 ccacttcagg cggttgtgcc acttcatcgg cagcgcgaac tggaagcgct tgaggccgat
     6481 cgcgccgccc accttgtaga gcagttccga cagcgagccg aacgggcaca gccagccgca
     6541 gaacaggccg cggccccaaa cgaacacggt gacgatgatg aagatccaga acaggaagat
     6601 gaacggatcg gtgaggaaca gctcccattc ccacttgaac agcagcgagt ggaaccaggt
     6661 cagcacctgc gtgatcgagg gctgggcgag caggccgaag ccgacaaaac cgatgctgat
     6721 gatccacgcg ctgtacttga aggcgttgac cggccacttg ttcttgcgcg tcgagcggcg
     6781 ggtcagttca tcgcgcttgg catagaccac cgccacggcg acgagcaagg ccgagaacag
     6841 gacgatctcg agcgcgcgcg tcttccacac gcggacccag ggcgcatcgg gcttcttgac
     6901 ctcgggccgg ccaccctcga tataggcgtc gggcagccag tattccctgt cgaagttgat
     6961 gaaggtgcgc gcaccggtct cgcggtcgat gcggttgccg aggaagatga acttccatgg
     7021 gaaggcgccg gagaacgagg acgagcgcac gatgaagatc gccgattcgg tgaaggacgg
     7081 cgcgcctggt gcggaaatcc catagaggtt gtagtagtcg aggtcgcgga aggtgaacac
     7141 gtcctgcccc tggcgaagct ggacacggtc gtagatgccc ccgcgcacga agcccgagcc
     7201 cttgaacgac tccctgcctg cggtgcggat gacgaaaagc gcgtgttcgc cctccttgat
     7261 gcgtgccatc aggtcgcgcc agccggtttc tcccatgatc gcgcggccga cctcgggatg
     7321 gttcagatag ccgtaccaga ggtcgatgaa cgggtccttg ccacgctcga ggccgacctg
     7381 ctcgggcatc acggtcaggc gttgcaccac gccttccttg accagtgtcg cccagtcgag
     7441 cttgcggccg ctatcgacga acctggcctg agggcggacg gtcttttcaa ggatgccgac
     7501 ctggcgcccg actgcagcgc ccgacatcat catcacctgg ttctgcgcga tgaccgtgac
     7561 ggtggcgccg gagatggcat cgaggccgat gatctcttcc tcggggcgcg agcggccgat
     7621 ctcgatgttg gcgccaacga acttgcccag gtattggttg ttgaacttga ccagcgcgga
     7681 ttcggggatg ccgagcagca ggatcggttc ggagtgcttg aggatcttga tgccggcgaa
     7741 gttgccttgc ggggtaagcc cgatcagcgt gatcacgggc ttgccggagt aggccggggt
     7801 gtcggtgacg tcggtcgata gcaccacgta gcccaccagc ttgcgttggc cgttttcctc
     7861 ggcgtagccc tcgacgtagg gcggctgacc cttgcgctcc gagaagctgg tcgcaccggg
     7921 caggacctcc ttgcagggca cgtagtcgca cagtgcgggc gcggtgttga cctcgggcgg
     7981 taacttggcc tcgtaggcac tttcggcgga ggcgctctgc ggcaggaggg tcgtgacggc
     8041 gagcagggcc gccatcgcaa tgccccgcag ggcggagagg ggcttcatga tgcgctccat
     8101 tagtaaatta cgatggagtt taagcggcga caccccgtac gtatttgtcc agggtcaaat
     8161 tgccgcaggg tgccgcagcg atcggcgaga gacgggcggc ggaaagcgat cgaggggctt
     8221 gcgcccctcg atcgtcagac ggcgccaccg acgagtggcg cggcgcagtg cgagcttacg
     8281 gacgcacgag catgcgcgta cgcatctcca ggtgcagcgc gtggcagaag tgcgtgcaat
     8341 agcaccagaa cacgcccggc ttgtcggcct tgaaggtgac ggacttcgtc tccagcgggt
     8401 tcaccacgaa gttgatgtcg tacttcggaa tcgcgaagcc gtgcgacagg tcttccacct
     8461 tgtcgaggtt ggtgaggatc agggtgacct cgtcgcccac cttgagctcg aactcgcgca
     8521 ggctgtaggc cggtgcctgc gaggcgatct tcaccgtgac cttgttgccg tcgcgggtca
     8581 cgcccgattc cttcgcgtcc ttgatggcca gcgggtgcgc ctcgagcacg ccgacctgac
     8641 gggtcttgat gatgtcgcgg ttgacgatga tgaagtcgtg cggctccggg tgcaccgggt
     8701 ggtcggccag cagcaccatc ttctcgccac ggatgtcgat cagctgctcg ttttccgggt
     8761 gcatcgggcc gaccggcagg aagcgatcct tcgagaactt gcaacccacc gccaggaact
     8821 ggccgctggc ttccttcgtc tcgcccatat tcgcgctgat gtggccgggc tgataatgca
     8881 cgtcgatgcg gtcgaccacg tattgcgcgg ccttgtcgcc attgtggaac tggatcgcct
     8941 tggcgatgtt ccatttgacg atctggctgt ccaggaagag cgtggtgaag gcgttgccgc
     9001 ggccgtcgta ggtggtgtgc agcgggccga ggccgacttc gacttcggcg accaccgcct
     9061 tgcggatgtc gtcgagcttg ccgtcgagga agtcggcgac cttggcgact tcgatgaccg
     9121 tgcaggtggg cgacagcttg cccgagcaga tcatgtactt gccgtccggg ctggcattga
     9181 cgccgtgcgg gttcttcggc accggcacgt aagcggtcag cgcggtcttc ggatcggggt
     9241 tggcggcctt ggtgccgtcg accaccggga ccttggaact gccgacggtg aaggacttgc
     9301 cgtccttgat ggccttctcg atgcgggcga cgtcgaagaa cacgcaggcg tccatctcgg
     9361 ccgacatcat gtcttcgtag cgcgcgccgc cctcggtgtt gtactggttg gagcaggcga
     9421 agcggccgtc ataggaagtg gcgacgaggt ccatgttgcc atcgaccttg cactgccagc
     9481 gcacttccat ggtctcggcg tcgacgcagg tgaacaggca gtagtacttg gtcggatcgt
     9541 cgaggtcgcg gccgtcgttg ggctgcggga tgtggaactc gttgccgcag aacacgcggg
     9601 tggtgtggtt gatcgcctga tcgaccgggt cgcgcttgtc cgagaagatg ccgtggaagc
     9661 cctggatgtt ggggatctcg gtgatcgcgt cgcattcgaa gatgtcgccg cggatacggc
     9721 cgaggcggcc gtggatcttg tcgttcaccc agaagtactt gccgtcgtag gtgccgtcgg
     9781 tgtacgaacc gtggacgtgg tgtgtgtcgc cggtcttgta cttgagctga ccgtccgggc
     9841 gggtgccgat gaccttcttc gactcgttgg tgatgcccca gccgctcatg cagtcgatgt
     9901 tgaaggtcgg gatgcgcttg atctcgcggc ccgagggcag gccgagcacg cggcattcac
     9961 cggagtggcc gccggaccac acgccgtagt aggtgtcgag ttcgcccggc gccatgtgca
    10021 gcttgaggtc ggaggcagca tgcgcggcgg cgggcgcggc ggccggtgct gtggcggggg
    10081 ccgcggccgg agcgggagcg gtttccttgt tgcacgcgga cagccccacc gacatgccgg
    10141 cgccagcgag gccggccagc gctgcggtgt tgaggaactt gcggcggctc ggatcgggca
    10201 tggcttcgac cgggttcatc tttgcgtcgt tcatggttct ttgctcccgt aggcaggtgt
    10261 agtcgtttct cacagacccg gcatcgcgac cttgcgcccc tcgcgtcggt tcttcatgcg
    10321 tgtcggaggc ctgtgacaaa ttgtcctgtg acgttttgtc gcaccccaat tctatgaagg
    10381 tggaaatcag taaaccttga tataaggcaa agatgagtcc ttcagttctt gtttgcgaag
    10441 ggaagaatgc agccgatcga gcaggaaacc gagcccgccg aggacctcga caaccccaat
    10501 ccggtactga tgcctgcgcg ggtgctcaac gccgtgcgca ccgatcgcgg gcgcctgctg
    10561 cagttgcgct gggtttccct gctcgcgatg gtgctgatga gcctggtcgt cttcccctgg
    10621 ctcgcgcccg cgcagccggt cgggccgctc gccggggtca ccttgtgcct gctcgcggtc
    10681 aacctcgccc tgctcggtgg ggcggcacgc tggctggtgg ggcgctgggg cgccttcctg
    10741 caactcgccg tggatctgct ggcatgggcg gcattcctct atttcaccgg tggcgtgacc
    10801 aaccctgcga tctcgctgct gctgccggtg gtggcggtgg gcgcttcgat cctgcccgcg
    10861 gcacaggcct gggcgctggc ggccctcgcg gtcgccctgt attccgtgct gtggcagttt
    10921 caccagccgg ttcgcctggc cgacgccgat caggcgatgt actggcacct ggccggcatg
    10981 tggatcagct tcgcggtctc cgccgccacc gtggtctggt tcatcgtgcg gctcaacgtg
    11041 gagctggcgc ggcgcgacaa cgaactcgcg gcggtcaacg cggcgcgtgc gcgcgacacc
    11101 tacgcggtcg gactgggcaa gctcgccgcc ggtgccgccc accgcctcgg cacaccgctc
    11161 ggcacgctgc gcattctcgc cgacgatctg gcgagccgac ccgagctcgc ctccgggctg
    11221 cgtgaagacg tgcagctgat gcgcgagcag gtcgactact gccgcgacat tctcaacggg
    11281 ctcacgcgcg aggccggcca gcagcgggcg gagagcggcg gggcggtcga ggtgcgggcc
    11341 tgggtgacgg aggtgggcgg gcgctggcag cgtctgcgcc ccggggcagg cttgcacatc
    11401 gatgcggagg cggcggacgg gcaggcggtc atcgtcgccg acgcatcgct cggcgatgcc
    11461 ctgcacaacc tcatcgacaa cgccgccaat gccaacgagc gcgcgggagc gcgcgctcgc
    11521 gcggtggagc tcgggatccg ccgcgagggc gcgcgggtgg ttgtcgaggt gctggatcgc
    11581 gggcccgggt tgccgccgga ccgcgtggag gcggcgcgtc tggcgcggct cggggagcgt
    11641 gcgcaaggca tgggcgtcgg cttgatgctg gcgcacgccg ccgtcgagca tcatggcggg
    11701 catcttgaat tccgttccag accgggtggt ggcacgattg cgcgcatgct gattccgcga
    11761 cagtccgtac gatgaccccc gacaacacgc cctgcctcct cgtcgtcgat gacgatccgg
    11821 ccttcaatcg cgtcctcgtg cgcgccctcg gccagcgtgg cttcgatacc tacggggcga
    11881 ccgacagcga gggcgcgctc gagctcgccc gcgaccacga gcccgagttc atcgtgctcg
    11941 acctcaacat cggcggcgag tcgggcctga agctcatccg ccccctgctc gaggcgagcc
    12001 ccggggcgcg tatcctcgtg ctcaccggtt acgccagcat cgccacggcg gtcgatgcgg
    12061 tgaagctggg ggccgtccag tacctggcca agccggccga catcgacagc atcatccgcg
    12121 ccttgcgcgc cgagggcgtg agaccagagg atcacgcgcc cggggctccg ctttcggttg
    12181 accgcctcga gtgggaacac atccagcgcg tgctcgcgga gcaccacggc aacatctcgg
    12241 caaccgcgcg tgcgctgcag atgcaccggc gcacgctgca acgcaagctc gcgcgcccgc
    12301 cggcgggggc ctgaccggca ggcgggtcgc gccggttccc ctctgttcaa ggacggcctt
    12361 ccgctctaac cttcgttccc gatcgaacct cccccagacc cccacaagga tcccatcatg
    12421 cgccgcttgc tcctgctgct gacgcccgtc ctcgctgcca ccgcgcatgc cagccccgtg
    12481 cctgccgagg cgccgatccg catggccggt ctgtggctga tgaacacgac ccccgtcggg
    12541 gccgcggcgc agacctccag cttccacatc tgcatcggcg cgcccgcgcg cgacgacgtg
    12601 ctcgcccacc ccggcagcgc cgtgcagaac tgccgcgaag agcgctggag caaggacgag
    12661 cactacacct actatcaggc gagctgcgag gccaagggcg gcaacgcggc ggtcgaggcg
    12721 aggtttgccg gtgacttcaa gtacaacttc cagggggagg tcaccatgac gatcgcgtcg
    12781 caagccggcg ctgccgaggt gaccaggatg gagatcgatg ggcggcggct ggcgccttgt
    12841 cgcggtggcc tgccggaagg caggttcctg atcaaggggc aggatggggt gggcaacctg
    12901 aatctgggcg aaccgattcg ccagccggcg cgctgaagca cgagccgttg ccgaccacgg
    12961 gagtcggtcg tcacagccac ggtccgcaga tcaccgagcg ggcagcggac gacctcgccg
    13021 ggtcctgcac cgcctcccca cggagcgatc acgacgtgcc tccgcgacga cagcattacc
    13081 gcggccgcaa cccggcgcgc gcccacagca tcgccgagct gcgcgcgatg gcagcgcgcc
    13141 ggctccccga cttctgcctc gaatacctcg atggcggcgc cgacgacgag ctcgcgctcg
    13201 cgcgcaaccg gcgcgtgctc gacgagatcc tgctgctgcc gcgcaccttg gtcgatgtgt
    13261 cgcagcgcga gcttgcggtg ccgctcttcg gcaccgacat cgcgctgccg gcggtgatcg
    13321 cgcccaccgg cttcaacggc ctgctcaccc acgcgggcga ccgcgtgctc gccgaggcgg
    13381 cgcacgcggc gggcatcccg ttctgccaga gcatggtgtc gaccgtggcg ctggaggaca
    13441 tcgccgccac cggtgtgcgc cactggatgc agatctatcc cttcaaggac cgcgacaacc
    13501 tcgccgcggt ggtgaagcgg gccgagcacg ccggctgcga ggcgatcgtg ctcaccaccg
    13561 acgcctcggt gttcggcaac cgcgaatggg accgccgcaa ctaccgcgcg ccgatgaagc
    13621 tggcctggcg caagctgctc gatgtcggcc ttcatccgcg ttgggtcctc gacgtgctgg
    13681 tgccgcacgg catgccgcgc ttccgcaacc tcggcgactt cctgccgccg ggcatggaca
    13741 gcgcgaagaa cgccgccgcc tttctcgccg cgcagatgga cacctcgctg acctgggagg
    13801 acctgcgccg cctgcgcgac ctgtggccgc gccggctgct ggtgaagggc gtgctgctgc
    13861 ccgaggatgc gctgcgtgcg caggaggcgg gtgccgacgg cgtggtggtg agcaaccacg
    13921 gcgggcgtca gctcgactgc gcgccggcgc cgatcgagac cctggccgcg gtgcgccagg
    13981 cggtgggggc ggagatgacg gtgatcgtgg atagcggctt tcgccgcggc agcgacttcg
    14041 tcaaggcgcg cgcgctcggt gccgatgcgg cgatgtcggg gcgggcgacg ctgtacggcc
    14101 ttgccgccgc gggtgccgcg ggcgcggcgc gcgcgttgga gatcctgcgc ggcgagatgg
    14161 agcgcacgct cgggctgatc ggctgtgcgc ggatggacga ggtggacgcg cgctacgtcg
    14221 gtcgcggctg aaggcctcgt ggggaggcgt gtgcctcaga ccccccagac caccggcgtc
    14281 tcctcgcgca gcgattcttc cagcatggcc agcagcggcc acgcgcgctg ggcgaggccg
    14341 accggggcgg ccatgccggt gttcccggct tccttctctg cctcctcgtc ggcgtggctg
    14401 cgctggcgtt cgagctcggc ctggcgggcg cgctcctcct cgatcgcggc cttgaggcgg
    14461 gcgatggcct cggggagttg ttgcacggtg acgatgccct tggcgtcggc ggggtccttg
    14521 ccgaggatgc cgatgagctt cttcgcggcg tcgccgaaca tgatcacgtc ggcgctcgcg
    14581 tgggacttga aggtgaccag catggggtgt cgctccttgt cttacaggcc cttgacggcg
    14641 tagatgccgg gcgcgttgcg ccagtagccc ttgtagtcca tgccgcagcc gaacaggaag
    14701 cggtcgggga tgtcgagccc ggtgaagtcg gcgcgcatgc cgggcctcgc cttgcgctcg
    14761 tgcagcttgt gcaccagcac cgcggacagc acctcggcgg cgccttcttc cttcaggtgc
    14821 gcgatgatgg cctcgagcgt gtggccttcg tcgaggatgt cgtcgagcac cagcaccgtg
    14881 cgtccgcgca ggtcctgggt cggacgcacg cgccagtcga gcagggtgcc ctggatggcg
    14941 tggccgtagc gcgtggcgtg caggtaggcg acttcgagcg ggaagggcag gcgcggcagg
    15001 atgcggccgg cgaggatcag cccgccgttc atcaccgcat agacgagcgg gttggcgtcg
    15061 gcgaggcgtg cgctgatctc ggccgccatg cggtcgagcg cggcctccac ggtggcggca
    15121 tcggcgaggc agtcggcctc ttcgcgggcg cgcttgattt cttccagatt cagggacatg
    15181 tcgatgaact gcgggcgctc gcccggtgcg tttgcggacg catcattcta gcgccgatcc
    15241 ccgcaaggcg ccgccttcga gggcgtgcgc ggatgagcgg cttgccgcga tgccgcgggc
    15301 tgtggtttgt gcgcttgtgc gcggaccagc ggcgcggtaa aagaggcggg tcttcgtcct
    15361 gccgccccgg aaaccgccat gcccatccat gaaatccgcc atccgctcgt gcgccacaag
    15421 atcggcctga tgcgcgaagg cgacatcagc accaagaagt tccgcgagct cactgccgag
    15481 atcgcccgcc tgctggctta cgaggcgtgc caggacttcc cgctcgagac ggtcgagatc
    15541 cggggctggg ccggtccggt cgagatcgag cagatcaagg gcaagaaggt caccgtggtg
    15601 cccatcctgc gcgccgggct gggcatgctc gacggcgtgc tcgacatggt gccaagcgcc
    15661 aaggtcagcg tggtcggcat cgcgcgtgac gaggagaccc tgcagccggt gccctacttc
    15721 gagaagtttg tcggccagct cgacgagcgc atggcgctga tcatcgaccc gatgctcgcc
    15781 accggcggct ccatggtcgc caccgtagac atgctgaagc gcaagggctg cacccacgtg
    15841 cgcgcgctgg tgctggtggc ggcgcccgag ggcatcgcgc tgatggacga gaagcatccc
    15901 gacgtggaca tctacaccgc gagcatcgac agccacctca acgagcacgg ctacatcatc
    15961 cccgggctgg gagatgccgg cgacaagatc ttcggcacgc ggcacaactg atcctgctgc
    16021 gggcggccgt gccgccgcca tcgcgtcctc actgcttcat cgataacgac aaccatgccg
    16081 gctgcgcgcc acgccgaccg cgtgcgcccg gcggagaacc ctcatgcgtg aaattcccat
    16141 ccagtctgcc gagccgctat ggcggcaggc gatctccggc gcccagatcc tcttcgtcgc
    16201 cttcggcgcg ctggtgctgg tgccgctgct caccggactg aacccttccc tggcgctgct
    16261 cggcgccggc gtcggcaccc tgctgttcca gctcctcacc gggcgccagg tgccgatctt
    16321 cctcggctcg agcttcgcct tcatcgcgcc gatcatcttc agcatccaga cctggggcat
    16381 cccggccacc atgggcgcgc tcgcctgcgt gtcgctgatg tacttcgtct tcgccgggct
    16441 ggtgtggaag cacggcgccg agatcgtgca ccgctacatg ccgccggtgg tggtggggcc
    16501 gatcatcatg atcatcggcc tgtcgcttgc cgcggtgggc gtcaacatgg cgatggggcg
    16561 cagcggcgac ggcaagctcg agcttgtgcc ctacgccacc gcgctcatgg tggcggggat
    16621 ctcgttcggc accaccgtgc tggtggccgt gttcgcgcgc ggcttcctca agctggtgcc
    16681 catcctgtgc ggcgtggcgg cgggctacgt cgcggcgatc ctgttcggcc tggtggattt
    16741 cgccgcggtg ggtgcggcgc cctggttcgt gctgccgcag ttcgtcacgc cgagcttcga
    16801 gctcgcggcc atcctgttca tgttgccggt ggccctggcg ccggcgatcg agcacgtcgg
    16861 cgacgtgctc gcgatcggtg gggtgaccgg caaggactat accgagaagc ccggcctgca
    16921 ccgcaccctg gccggcgacg gcgccggcgt gctgttcgcc ggcctggtcg gcggcccccc
    16981 gatcaccacc tattccgagg tcaccggcgc gctgaccctg accaagaact acaacccggc
    17041 gatcatgacc tgggccgcgg tgttcgcggt gatcctcgcc ttcttcggca agttcaacgc
    17101 gctgctggcg tcgatcccgg tgccggtgat gggcggcatc atgatgctga tgttcggctc
    17161 ggtggcggcc gtcgggctga acaccctgat caaggcgcgt gtcgacctgg aggagccgcg
    17221 caacctggtg atcgtgtctt cggtgctggt gatgggcatc ggcggtctgg cgctgaactt
    17281 cgacggcctg accctgcagg gcgtcagctt gtgcgccgtc gtcgcggtgg tgctgaacct
    17341 gatcctgccg aacgctcgag ccggcgcctg agcagcgcga gcaggccgcg caggagcggg
    17401 acggcggtgg gcagcagcgc cgagagccgc ccgccgcgcg cctggcgaag cagcagccag
    17461 ccgacccagg cgcgcttggc gagccgcagc gtgagtcgcg gccttgcagc gagcacgacg
    17521 agcagtgcga ccgacagcac ggggaccttg tccttcgccc acgcgaggtg atcgcgcagc
    17581 gcatcgacgc gatcgagcgc gccgtcgatg ccggcgaggc gctgcgtgat gtcggcgcgc
    17641 tgggcctcgg cgcgcagctg cagcttctgc ttgcgcagcg cgagttcgac ctggcgggga
    17701 tccatgcgcg gcgcgccttc agtcgggttt cttcaaggcc gcgcggtcgc gggcgagctc
    17761 ggccaggctg gccgagaaca gcttcgtgcc gctacggaag gcgcgcgccg cgttactcgc
    17821 cgcgagcagc ccgccgccga ggcagatcgc agtggagatg ccgagcgcga gcaggcgatg
    17881 ttcgtcccag aacagcacgg tcaggaacac catcagaaac accagcccga agccgagcag
    17941 cagccccgcc agcaccgtgt tgagcgcaag cccggtgagg cggagctttt cttcctgaag
    18001 ctcgaccgca agcagttcca gccgcgtctg cgccgtctgc agcccggcat cgacgatgcc
    18061 gtgcaggctt tcggcgagac gcggtctggg cggcctgggc tcggtgctca tcgaatcagc
    18121 gacggctgac cagcaggccg agcaggaagg cgacgccggc ggcgacgccg accgactgcc
    18181 aggggttttc gtgcacgtag tcgtcggttg cgcgcacggc cttcttgccg gagtcgacga
    18241 tcgcggcctc ggcatcggcc aggcggtcct tggcgaccgc gacgcgaccg ttgatgcgcg
    18301 cgcgcagatc atcgagcttg ccgccctgct ggtcggcggt gagcttgagg agttcctccg
    18361 cgtccgaaat cagggacttg aggtcgtcga cgatcttgtc cttggagggc tgaagggtcg
    18421 ttgccatggt ggttctcctg taagtggtcc gggaaaagtg gcctgaagcg cttcgtgcca
    18481 tgcctgaagc aagcctaccc cgcggggcgt ccggccgcaa tgaacgatgc gtaagcgagt
    18541 gctgaagccg ctgcag
//
LOCUS       DAOQNN010000019        18150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1145_length_18150_cov_10.466593, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000019 DAOQNN010000000
VERSION     DAOQNN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 18150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18150
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1145_length_18150_cov_10.466593"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PKV97_02500"
     CDS             <1..1065
                     /locus_tag="PKV97_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNB04775.1"
                     /translation="DSEPAAVAEAPMLDIARINQQSLRLLHLALLSGLAVALYWVWAD
                     LLTVVSYLDNITLYEYASGSGETAGVVPISLADLLGALVIAAIAFMLATNLPGLLEVL
                     VLSRLQLAQGSAYAITTLLSYVIVGVGIASTLGTLGVSWDKLQWLVAALSVGLGFGLQ
                     EIFANFVSGLIILFERPLRIGDVVTIGNVSGTVSRIQIRATTITDFDRKEMIVPNKAF
                     ITGQLTNWSLTDTVTRVTIKLGVAYGADLETTRRLLLAIAAENARVLKDPEPLVFFLN
                     FGPSTLDHELRVHVRELGDRNAAIDEINREIDRRFREAGIEIAFNQMDVHIRSIADGR
                     EALLQQTAVTPPAGSPGQQG"
     gene            complement(1104..1490)
                     /locus_tag="PKV97_02505"
     CDS             complement(1104..1490)
                     /locus_tag="PKV97_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04776.1"
                     /translation="MYRDPRFSGIFQTLGGPAAKPPTLLQKVVGAVVMAGVFMLALTF
                     SVAFFAVLLTAGVAIWGWVWWKTRALRKAMRAQMDAQAAAGGARAGGYGPAAGNRGLI
                     IEGEVIREVEIEEDPRRDGPGGRTPS"
     gene            1684..2361
                     /locus_tag="PKV97_02510"
     CDS             1684..2361
                     /locus_tag="PKV97_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB04777.1"
                     /translation="MPALALHQLRTRHIGPVDLDVAAGECVCIRGASGSGKSLLLRAI
                     ADLDPHAGEALLDGRACSSMPGPAWRRRVALVMAEGQWWADTVGEHFPAGCTPAWLDR
                     VGLPAGALDWQVARCSTGERQRLALLRTLMQGPAVLLLDEPTGNLDEDSTARVEAVLD
                     DYRREHAAALLWVSHDPRQAARVARRRFLLEDGKLVEEEEGGKAAADKGKAAGADRPP
                     DAEGAAW"
     gene            2355..3155
                     /gene="fetB"
                     /locus_tag="PKV97_02515"
     CDS             2355..3155
                     /gene="fetB"
                     /locus_tag="PKV97_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HNB04778.1"
                     /translation="MVMELSVLDLALAALLILALAGLSLRLGLGIEQRLVVAAARTTI
                     QLLLIGLVLKLLFDNAHFLWVAAMAAVMITAAGREVMARQKRRFVGWWGFGLGTLSMF
                     VSSFTVSVLALLVVIGNTPWYAPQYAIPLLGMVLGNTMNGIALGLDRLTQEASARRTV
                     LETRLALGQNWNETIADTRREAIRVGMIPIINAMAAAGIVSLPGMMTGQILAGTAPVE
                     AVKYQILVMFLIAGGTGFGTVVAVSLGARRLFDERQRLRLDRLRTGAD"
     gene            3169..4512
                     /locus_tag="PKV97_02520"
     CDS             3169..4512
                     /locus_tag="PKV97_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNB04779.1"
                     /translation="MNGPGARTATSRRPLSSAQAAAGATPRVGQPSPLILFGAFDRHN
                     LGDLLLGRIAAELAARLLPGRELRFAGLAVRDLHADGGARVEALAELAAEWKPPRRDT
                     PPPGLLQVGGEILDCSAWEAAVMLLDNAGAARAIALYDHDPAARAAWARHTLGVPRGL
                     PYLAAHAALPPGTRSVHTGIGGVGFAALPAALRAEALAELRAAAGVQVRDRCIRDALA
                     AAGLDAALVPDPAVLVERLLGAEVAHHAATGEVAALRARFARGYVAVQLAAEFGDDAT
                     LATLAGELAAIQRERDLGIVLFCAGRAPWHDDPDVLQRLRRRLPDPEAAVIAASPHVM
                     DLCALIANAALCCASSLHARIVADAFARPAIGLLPGGAPQAAKLRAWLDTWHPPRRHH
                     LAASGNLRALARSVLDEAPAERKVQAVRLADLAETAARAAFARLFDPIDPEDKTP"
     gene            4509..5585
                     /locus_tag="PKV97_02525"
     CDS             4509..5585
                     /locus_tag="PKV97_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HNB04780.1"
                     /translation="MTDLFTPLQAGDLALPNRVLMAPLTRCRAEDGHVPGALMATHYA
                     QRASAGLLIAEATMAMEGNSAFWHEPGIHSEAQVAGWKKVTDAVHAAGGRIVLQIWHG
                     GRACHPLLNDGRQPVAPSALAITGDEVHTPQGKQPYVVPRELRDDELPGIVAGFARAA
                     ENARAAGFDGVEVHGANGYLLDEFLRDGSNQRGGPYGGPRENRARLLFEVLDAVCKVW
                     GGGRVGVRLSPLNSYNDMIDSDPIGLASWLAGCLNDWRLAYLHLMRGDFFGQQHGDVV
                     TPVRAAYRGVLIGNMGYSADEANAAIAAGKLDAVAFGTAFLANPDLPERIRRKAALNA
                     PDPATFYTPGPKGYTDYPTLADGA"
     gene            complement(5625..6683)
                     /locus_tag="PKV97_02530"
     CDS             complement(5625..6683)
                     /locus_tag="PKV97_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HNB04781.1"
                     /translation="MSALSTLRLAAWRQILPTLALATLAGWLAERLHLPLPWMIGPLF
                     AVAGARMADRDLRPLPGGRQTGQWAIGAALGLYFSPEVVAQLSLHAPLVIAAALGSLV
                     LGMAYAFILLRLGGVDAPTAFFAALPGGASEMANLADRHGGAVDRIAAAHAIRVMMVV
                     SVLPFALTFAGVQGSDVYVPLARDVDAQHFPLLVGASLLGVGAFKLLRIANAWVLGPL
                     LGVALATLAGVPLSALPPWVVNGGQLLIGCALGCRFSRAFFRAAPRFMSVAALTGAMS
                     IVLAFALATLLGLVAEVPLSTLALATAPGGVSEMCITAKVLQLGVPLVTVCHVLRVVV
                     LTVGAQWSFGIFRRLVAV"
     gene            complement(6809..7915)
                     /locus_tag="PKV97_02535"
     CDS             complement(6809..7915)
                     /locus_tag="PKV97_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB04782.1"
                     /translation="MRIRGGRAALKTVLAVVAAVAIAPVRAEPIADRFDQARLEGVVV
                     VYAATDLEVVKPVIDDFEALHPGVRVQYHDMHSAELHARVVDEARRGLAGADVVWSSA
                     MDLQVKLVNDGHAQPHRSAETAALPRWAVWKDEAFGTTYEPAVIVYNKRLLGEADVPD
                     SHAELIRLLDRDPAPLRGRIATYDPERSGLGLLLHTQDAQANPIVFWQLARGMGRQGL
                     EQHAASSEMLDRVAAGKLVLAYNVLGSYAHRRARSDPALGVALPRDYTLVLSRVAFIM
                     RGARHPAAARLWLDHLLSTRGQALLAANLGLLPVRTDAGTAGADSAAALLHHNLQHAF
                     RPIRIGSGLLAYQDQAKKQAFLRQWDAATRPVSE"
     gene            8059..8748
                     /locus_tag="PKV97_02540"
     CDS             8059..8748
                     /locus_tag="PKV97_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB04783.1"
                     /translation="MRLLLVEDHAELAEWVARSLRHAGFVLDVLNRGDHADQSLRTER
                     YDLVILDLSLPGLDGLEVLRRLRNRESGPDKPAPTPVLVLTARGSTEERVHGLNLGAD
                     DYLPKPFELAELEARIKALLRRAGNQVPLVRLGRLEFDTTTRMAAVDGQALALTPREL
                     AVLEALLARVGRPVAREALFEKVFEFDADARVEAIEIYVSRLRKKLEGAGVAINTLRG
                     LGYLLAEKPAA"
     gene            8750..10240
                     /locus_tag="PKV97_02545"
     CDS             8750..10240
                     /locus_tag="PKV97_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HNB04784.1"
                     /translation="MDGASGSGPANPGTDDREHGTAARAVAAPGHGGSLRQRVLVWLL
                     PPLAVLLLINAFFTHRSALDAVNQAYDRTLTASVRAIGERTHSLGGQIQVDIPYAAFE
                     GFEADAAERIFHAVFDPAGGLVTGYADLRPPELPADDGSVRIFDLTHAGETVRLAAMR
                     KRLYDPALRGGDAVLIVFAETTGARRALAHDLFVASLQRQSLLVAVGAILVLAALGTA
                     FRPLLRVRDHMRARDEEDLTPVRMERVPSEIAPLIDAINHHMERLSAMLAARRRFLAD
                     AAHQIRTPLAVLTTQAEYGLRQTDPEEMLATFASLLRSTRAARRLANQMLTLSRAEAV
                     NGLIGERAALDLAGLVKEVAMELAPLALKRDIDLAYEGIGHALTMQGNAPMLREMVSN
                     LIDNAIRYSPPGSAVTVAVAAFGEDAVVSVCDQGPGIPTAERERVFQRFYRILGQGDS
                     EGSGLGLAIVREIALAHGGRVTLRDGAGGRGLCVEVELPLGSTPSA"
     gene            10363..11406
                     /locus_tag="PKV97_02550"
     CDS             10363..11406
                     /locus_tag="PKV97_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNB04785.1"
                     /translation="MQRLSAPALVAAALALPSLALAQSNVTLYGLIDLNLGYEKSGSQ
                     RYEGIGHGELNGSRLGFRGTEDLGNGNKALFVLESGFDPSTGVAEQGGRLFGRQSFVG
                     LENGFGRVTLGRQYSPAFDALDPFDATGNADRSAGLLIRKAGAVKPAYETRFDNMIKA
                     RSAKLAGFELEGGYWFGKESGTDSTARQEGDGHGIAMLYANGALAGSLVTQTVQRDAS
                     GGKVRTDGFALSYDFGIVKPYFAWSQDKERGSVGNGKARSWDLSAEIRLDPANTVAIS
                     YADRDESDGAASEDASGWSAYYLHALSKRTTLYAGYSQLDNEVGANYAIGNLTPAAGD
                     KHQVMMAGMRHRF"
     gene            11607..12023
                     /locus_tag="PKV97_02555"
     CDS             11607..12023
                     /locus_tag="PKV97_02555"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB04786.1"
                     /translation="MNPHETPSQPRGEPAIRTIAMPADANPAGDIFGGWLMAQMDLAA
                     GNVAARRARSRCATVSVDRITFLRPVYIGDEVSLYADIVRVGRSSMTIQVEAWRRSRD
                     GEEQVKVTDASFVFVALDQGGRPRVIDPVSGPQAEH"
     gene            complement(12162..13664)
                     /locus_tag="PKV97_02560"
     CDS             complement(12162..13664)
                     /locus_tag="PKV97_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNB04787.1"
                     /translation="METLSLLMDGFAVAGTPENLMIALIGCFIGTVVGLLPGLGPING
                     VAILLPIAFAMKLPPESALILLCAVYAGCEYGGRISAILINVPGDAGAVMTTLDGYPM
                     AKKGLAGPALSMSALASFTGALLATIGIVAFAPLLAEWAIAFGPAEYFALMVLALSCL
                     GGLVGDSLPKAMTACLIGLLLSTVGIDANSGVYRYTFDMLHLQDGIPFIVIVIGLFAV
                     SELMLMLQNEHTASEPITITGRILFNMKELMLAKWTMLRSAFSGFFIGVLPGAGATIA
                     SAMCYAMERRMAGKDHQFGNGDIRGVAAPEAANNASANGSLVPMLTLGVPGSGTTAVM
                     IGALTLYNITPGPTLFQDQPVIVWGLIASLFIANVMLLVLNIPLIRVFVKFLSIPNWL
                     LVPGVAAISLVGVYAVHGTTFDLILGTLIGGVGYLLRVMNFPIAPFILGFVLGDMMEQ
                     NLRRALSISDGDVGVLFASPISIGLWVAAAAMLLGPRLMRMMEQRKAVAA"
     gene            complement(13668..14111)
                     /locus_tag="PKV97_02565"
     CDS             complement(13668..14111)
                     /locus_tag="PKV97_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HNB04788.1"
                     /translation="MSERIFGGVLLLVSLAGIWIGWDLQAPMSYEPVGPRAFPLLVLF
                     LLGLCAIALMLEKTSETVWAPPPVLKRIGLLFLIVLAYALLFDKLGFTIATALMTVPV
                     ARFFGGTWKQGVVGGIGLGVGMFFFFDRLLDVALPTGLWLNGILG"
     gene            complement(14186..15166)
                     /locus_tag="PKV97_02570"
     CDS             complement(14186..15166)
                     /locus_tag="PKV97_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNB04789.1"
                     /translation="MLLKKTLFAAATAVLATLAAGVVHAAPEKPECIAPAKPGGGFDL
                     TCKLVQSALQNGYLEAPMRVTYMPGGIGAVAYNTVIANRPAEAGTVVAFSGGSFLNLA
                     IGKFGKYTEDDVKWVAAVGADYGAIIVRADSPFQSLKDLIEATRANPGKVVYGAGGSV
                     GSQDWMKSALVAREAGVDPKSMRYVAFEGGGEAMTALMGGHVQVYSGDASEVAAHLKG
                     GRVRVLAVLAPERLPGELAAIPTAIEQGYKVDWTIARGYYVGPKVADADYAWWVATFD
                     KMLASPEFDKLRAERGLFPFARTGAAATEYVKQEVQRFKKLAVELGLTAQ"
     gene            complement(15305..16255)
                     /locus_tag="PKV97_02575"
     CDS             complement(15305..16255)
                     /locus_tag="PKV97_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNB04790.1"
                     /translation="MSGFLELLDFSLGVTGPIFTLLGLGVLLRRVGLITEGFIAAGSR
                     LVFVVGLPTMLFVNIARTRLEDSASAGMLVYGLAATVAIWLLMEWVASFVVQPERDRG
                     VVVQGAFRSNFGVVGLAYCGNAYGEAGLAMASLYVGVLTILVNILGVITLSRSLHRSQ
                     GPGKVLRGIATNPLILGILLALPVSAFALPLPAVAMKSAAYIADMTLPLALLCTGATL
                     DFRSLRSEAGNTALATFGKLVAMPLVFTLGAWAAGFRAMELGVLMLMASSPSAAASYS
                     MVRAMGGNATLAANIIALTTLGSLFTTSVGVSLLRGVGLM"
     gene            complement(16255..17190)
                     /locus_tag="PKV97_02580"
     CDS             complement(16255..17190)
                     /locus_tag="PKV97_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNB04791.1"
                     /translation="MDIIFGIILPVFGTLGLGYVAARSGVFDEAANRGLSVFVFNFAL
                     PLMLFRAIAQAELPDAMPWGYLLSYYIGAFGVYALAMLGGRVLFGRRLGEQAVLGLGA
                     GFSNTGMLGIPLVMTAYGPPAALPLFVMIACHSLLMLPPTTALIEAAQGAREGLGPML
                     LKLTKSVLGTPIIWGLSCGLAFALLGIDIPGPLDAVAKGLGSAATPCALFALGASLTR
                     YSLGGNLREPALLVALKAVVHPAIVWLLATQVFAVPPLWVATGVLLAALPAGITPYLF
                     AQRYGACQSTVASAVFLSTLVSVGTLSVLLFVLRP"
     gene            complement(17375..>18150)
                     /locus_tag="PKV97_02585"
     CDS             complement(17375..>18150)
                     /locus_tag="PKV97_02585"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNB04792.1"
                     /translation="AGARHLIVGIGGSATNDGDAGMGQALGARLLDAQGGELGPGGGA
                     LAALERIDVSALDPRLAQCRIEVACDVDNPLTGPRGASAVFGPQKGASVEMVQALDAN
                     LARFARIVERDLGVAVEAVAGAGAAGGMGAAMLAFFGATLKPGIEIVTAAVGLDAHVR
                     DADLVITGEGRIDFQTVHGKTPIGVARVAKRHGIPVIGIAGSLGADVGVVHAHGIDAV
                     FSVLGKPCTLDEALHDAAANVELTARNVAAVLRIGLARP"
BASE COUNT         2856 a         6619 c         6275 g         2400 t
ORIGIN      
        1 gacagcgagc cggctgcggt ggccgaggcg ccgatgctcg acatcgcccg catcaaccag
       61 cagtcgctgc gcctgctcca cctggccctg ctgtccggcc tcgcggtggc gctgtactgg
      121 gtgtgggccg acctgctgac ggtggtgtcc tacctcgaca acatcaccct gtacgagtac
      181 gccagcggca gtggcgagac cgccggcgtg gtgccgatca gtctcgccga cctgctcggc
      241 gcgctggtga tcgcggcgat cgccttcatg ctcgccacca acctgccggg tctgctcgag
      301 gtgctggtgc tgtcgcggct ccagctcgcc cagggcagcg catatgcgat caccacgctg
      361 ctctcctacg tcatcgtcgg cgtcggcatc gcttccacgc tgggcaccct gggggtgagc
      421 tgggacaagc tgcagtggct ggtggcggcg ctgtcggtgg gcctgggctt cggcctgcag
      481 gagatcttcg ccaacttcgt ctccggtctg atcatcctgt tcgagcgccc gctgcgcatc
      541 ggcgacgtgg tgacgatcgg caacgtctcg gggacggtga gccgcatcca gatccgcgcc
      601 accacgatca ccgacttcga ccgcaaggag atgatcgtgc ccaacaaggc cttcatcacc
      661 ggccagctca ccaactggtc gctgaccgac acggtgacgc gggtgacgat caagctcggc
      721 gtggcctacg gcgccgacct ggagacgacg cggcgcctgc tgctcgcgat cgcggccgaa
      781 aacgcgcgcg tgctgaagga tccggaaccg ctggtgttct tcctcaactt cggccccagc
      841 acgctcgacc acgagctccg ggtgcacgtg cgcgagctcg gcgaccgcaa cgccgcgatc
      901 gacgagatca accgcgagat cgaccgccgc ttccgtgaag cgggaatcga gatcgccttc
      961 aaccagatgg atgtgcacat ccgcagcatc gccgacggcc gcgaggcctt gctgcagcaa
     1021 acggcggtga ctccgcccgc cggttcaccg ggtcaacaag ggtagatggg aacgcggctg
     1081 cgccgggcgt ggggcgactg gcgtcacgac ggggtgcggc cgccggggcc gtccctgcgc
     1141 ggatcctcct cgatctccac ctcgcggatc acctcgccct cgatgatcag cccgcgattg
     1201 ccagcggcgg gaccgtagcc gcccgcacgg gcaccgcctg cggccgcctg cgcgtccatc
     1261 tgcgcccgca tcgccttgcg cagggcacgc gtcttccacc acacccagcc ccagatcgcc
     1321 acgccggcgg tcagcagcac cgcgaagaag gcgaccgaga aggtcagcgc gagcatgaag
     1381 acgcccgcca tgacgaccgc gcccaccacc ttctgcagca gcgtgggcgg cttggcggcc
     1441 ggcccgccga gggtctggaa gatgccggag aatcggggat ccctgtacat gagtcggtcc
     1501 tgtcgggcgg cgcggaccgt ccgcgccgca tgcgggggtg tgagcccctg gcggaaaaaa
     1561 gattccatgc aggcgaagat gtgcatggat tgcttgcaca ttctcgccca atcggagcgc
     1621 gcttgccgag cgcggcgagc cacccgcatt gaaaaccccg cccggacacg cgaacatccg
     1681 tccatgcccg ccctcgccct gcaccagctc cgcacccgcc acatcggccc ggtcgatctc
     1741 gacgtcgccg ccggcgaatg tgtctgcatc cgcggcgcct cgggcagcgg caagtccctg
     1801 ctgctgcgcg cgatcgccga tctcgacccg cacgcgggcg aggccctgct ggatgggcgc
     1861 gcgtgctcgt ccatgccggg cccggcctgg cggcgccggg tggcgctggt gatggccgag
     1921 ggccagtggt gggccgatac cgtcggcgag cactttcccg ccggctgcac gcccgcctgg
     1981 ctggaccgcg tgggcctgcc cgccggcgcg ctcgactggc aggtggcgcg ctgctccacg
     2041 ggcgagcgcc agcgcctggc actgctgcgc acgctgatgc aggggccggc ggtgctgctg
     2101 ctcgacgaac ccaccggcaa cctcgacgag gacagcaccg cgcgcgtcga ggccgtgctc
     2161 gacgactacc ggcgcgagca cgccgccgcg ctgctgtggg tcagccacga cccgcgccag
     2221 gccgcacggg tggcgcggcg ccgcttcctg ctcgaggacg gcaagctggt ggaggaagag
     2281 gaaggcggga aggcggccgc ggacaaagga aaggctgcag gcgcggacag gccccccgac
     2341 gccgagggcg cggcatggtg atggagctct cggtcctcga ccttgcgctc gccgcgctgc
     2401 tgatcctggc gctggccggg ctgtcgctgc gcctgggcct gggcatcgag cagcgcctgg
     2461 tggtggcggc cgcgcgcacc acgatccagt tgctgctgat cggtctggtg ctgaagctgc
     2521 tgttcgacaa cgcgcacttc ctgtgggtgg cggcgatggc cgcggtgatg atcaccgccg
     2581 ccgggcgcga ggtgatggcg cggcagaagc gccgcttcgt cgggtggtgg ggcttcggcc
     2641 tgggcacgct gtcgatgttc gtgtcctcct ttaccgtcag cgtgctggcg ctgctggtgg
     2701 tgatcggcaa cacgccgtgg tatgcgcccc agtacgcgat cccgctgctc ggcatggtgc
     2761 tcggcaacac catgaacggc atcgccctcg ggctggaccg cctgacccag gaggcgagcg
     2821 cgcggcgcac ggtgctcgag acccggctcg ccctcggcca gaactggaac gagaccatcg
     2881 ccgacacccg gcgcgaggcg atccgcgtcg gcatgatccc gatcatcaac gccatggccg
     2941 ccgccggcat cgtcagcctg cccggcatga tgaccggcca gatcctcgcc ggcaccgcgc
     3001 cggtggaggc ggtgaaatac cagatcctgg tgatgttcct gatcgccggt ggcaccggct
     3061 tcggtaccgt ggtggcggtg agcctgggcg cgcgccggct cttcgacgag cgccagcgcc
     3121 tgcgcctcga ccgcctgcgc accggcgcgg attgaggcag ggggggcgat gaacgggccc
     3181 ggcgcgcgca ccgcgacatc ccgccgcccc ctctcgtccg cgcaggcggc ggccggcgcc
     3241 acgccccggg tcgggcagcc ctcgccgctg atcctgttcg gtgccttcga ccgccacaac
     3301 ctcggcgacc tgctgctcgg ccgcatcgca gcggaactgg ccgcgcgcct cctgcccgga
     3361 cgggagctgc gcttcgccgg cctcgccgtg cgcgatctgc atgcggacgg cggtgcccgc
     3421 gtggaagcgc tcgccgaact cgccgcggaa tggaagccgc cccgtcgcga cacgcccccg
     3481 ccggggctgc tgcaggtggg tggcgagatc ctcgactgca gcgcctggga ggcggcggtg
     3541 atgctgctcg acaacgccgg cgccgcacgc gcgatcgcgc tatacgacca cgaccccgcc
     3601 gcgcgcgcgg cctgggcacg gcacacgctc ggggttccgc gtggtctgcc ctacctcgct
     3661 gcgcatgccg cgctgccgcc cggcacgcgc agcgtgcaca ccggcatcgg cggcgtcggc
     3721 ttcgcggccc tgcccgcggc gctgcgtgcc gaggcgcttg ccgagctgcg cgcagccgca
     3781 ggggtgcagg tgcgcgaccg ctgcatccgc gacgccctcg ccgccgcggg cctcgacgcg
     3841 gcgctggtcc ccgaccccgc ggtgctggtc gaacgcctgc tcggcgccga ggtggcgcac
     3901 cacgccgcga cgggcgaggt cgcggcgctg cgggcacgct tcgcacgagg ctacgtggcg
     3961 gtgcagctcg cggcagagtt cggcgacgac gccaccctcg ccacgctcgc aggcgagctt
     4021 gcggcgatcc agcgcgagcg cgacctgggc atcgtgctgt tctgcgccgg gcgtgcgccc
     4081 tggcacgacg accccgacgt actgcagcgc ctgcgccgcc gcctgcccga tccggaggcg
     4141 gccgtcatcg ccgcctcccc ccacgtcatg gatctgtgcg cgttgatcgc caacgccgcg
     4201 ctatgctgcg cgagcagcct gcacgcacgc atcgtcgccg acgccttcgc gcgtcccgcg
     4261 atcggcctcc ttccaggcgg ggcaccgcag gccgcgaagc tgcgcgcctg gctcgacacc
     4321 tggcacccgc cacgacgcca tcacctggct gcgtcgggga acctccgcgc gctcgcgcgc
     4381 tccgtgctgg acgaagcgcc cgccgagcgc aaggtgcagg ccgtgcgtct cgccgacctc
     4441 gccgagacgg cggcgcgcgc agccttcgcc cgacttttcg atccgatcga ccccgaggac
     4501 aagaccccat gaccgatctc ttcaccccgc tgcaggcggg cgatctcgcc ctgcccaacc
     4561 gcgtcctgat ggccccgctc acgcgctgcc gcgccgagga cggccacgtg cccggcgcgc
     4621 tgatggccac ccactacgcc cagcgcgcca gcgccggcct gctgatcgcc gaggcgacga
     4681 tggcgatgga gggcaactcc gccttctggc acgagcccgg catccactcc gaggcccagg
     4741 tcgcaggctg gaagaaggtc accgacgcgg tgcatgccgc cggcgggcgc atcgtattgc
     4801 agatctggca cggcggccgc gcctgccacc cgctgctcaa cgacggccgc cagccggtcg
     4861 cgcccagcgc gctcgcgatc accggcgacg aggtgcacac gccccagggc aagcagccct
     4921 atgtggtgcc gcgcgaactg cgcgacgacg agctgccggg catcgtcgcc ggcttcgcac
     4981 gcgccgccga gaacgcccgc gccgccggct tcgacggcgt ggaagtgcac ggcgccaacg
     5041 gctacctgct cgacgagttc ctgcgcgacg gcagcaacca gcgcggcggt ccctacggcg
     5101 gaccgcgcga gaaccgcgcc cgcctgctct tcgaggtgct cgacgcggtg tgcaaggtgt
     5161 ggggcggcgg ccgcgtcggc gtgcgcctgt cgccgctcaa cagctacaac gacatgatcg
     5221 acagcgaccc gatcgggttg gcaagctggc tcgccggctg cctgaacgac tggcggctcg
     5281 cttacctgca cctgatgcgc ggcgatttct tcggccagca gcacggcgac gtggtgacac
     5341 cggtgcgcgc cgcctaccgt ggcgtgctga tcggcaacat gggctactcg gcggacgaag
     5401 cgaacgccgc gatcgccgcc ggcaagctcg acgcggtcgc cttcggcacc gccttcctcg
     5461 ccaaccccga cctgcccgaa cgcatccgcc gcaaggccgc gctcaacgcg cccgatccgg
     5521 cgaccttcta cacccccggg ccgaagggat acaccgatta cccgacgctg gcggacggcg
     5581 cctgatcccg cgccggtacg cccgcgcggg cgtaccggcg ccgctcagac ggccaccagg
     5641 cggcggaaga tgccgaagct ccactgcgca cccacggtca gcaccaccac gcgcaacaca
     5701 tggcacacgg tgaccagggg tacgcccagc tgcagcacct tggcggtgat gcacatctcc
     5761 gacactccgc cgggagcggt cgccagcgcc agcgtcgaca gcggcacctc cgcgaccagg
     5821 ccgagcaggg tcgcgagggc gaacgcaagc acgatcgaca tcgccccggt gagcgccgcg
     5881 acgctcatga agcgtggcgc ggcgcggaag aaggcacgcg agaagcgaca gccgagggca
     5941 cagccgatca gcagctgacc gccattgacg acccagggcg gcagcgccga cagcggcacg
     6001 ccggccagcg tcgccagcgc cacgccgagc agcggcccga gcacccacgc attggcgatg
     6061 cgcagcagct tgaacgcccc caccccgagc aggctcgcgc ccaccagcag cgggaagtgc
     6121 tgcgcgtcca catcccgcgc caggggcacg tagacgtcgc tgccctgcac cccggcgaag
     6181 gtgagcgcga agggcagcac cgacaccacc atcatgaccc gaatggcgtg cgccgcggcg
     6241 atgcggtcca ccgcaccgcc gtggcgatcg gcgaggttgg ccatctccga ggccccgccc
     6301 ggcaaggcgg cgaagaacgc ggtcggcgca tccacgcccc ccagccgcag cagaatgaag
     6361 gcgtaggcca tgccgagcac cagcgagccc agcgcggccg cgatcaccag cggcgcatgc
     6421 aggctcagtt gcgccaccac ctccggactg aagtacagcc cgagcgcggc gccgatcgcc
     6481 cattgtcccg tctgccgtcc accggggagc gggcgcaggt cccggtcggc catgcgtgcg
     6541 ccggccacgg cgaacagcgg gccgatcatc cacggcagcg gcaggtgcag gcgttcggcc
     6601 agccagccgg cgagcgtggc gagcgcgagc gtgggaagga tctggcgcca ggcggcgaga
     6661 cggagcgtgg agagggcgga catcggcagg agatcggcga gagatcggga cgaaacggag
     6721 aaagggccgc tggggacgca ccgcgatccg ggaccacgct gcgcggcggt gggggagagg
     6781 accggggatc cggtcggccg ggcgggcctc actcggaaac cggccgcgtc gccgcatccc
     6841 actggcgcag gaaggcctgc ttcttggcct ggtcctggta ggcgagcagt ccggagccga
     6901 tgcggatcgg gcggaaggca tgctggaggt tgtggtggag cagcgcggca gcgctgtccg
     6961 cgcccgcggt gccggcgtcg gtgcgcaccg gcagcaggcc gaggttggcg gcgagcaggg
     7021 cctggcctcg ggtcgacagc agatgatcga gccacaggcg cgccgccgcc gggtgacggg
     7081 cgccgcgcat gatgaaggcg acccggctga gcaccagggt gtaatcccgc ggcagcgcca
     7141 caccgagcgc cgggtcgctg cgcgcccgcc ggtgcgcgta cgagcccagc acgttgtagg
     7201 cgagcaccag cttgcccgcg gcgacgcggt cgagcatctc gctgctcgcc gcgtgctgct
     7261 cgaggccctg ccggcccatg ccgcgcgcga gctgccagaa cacgatcggg ttggcctgcg
     7321 cgtcctgcgt gtgcagcagc aggccgaggc cggagcgctc ggggtcgtag gtggcgatgc
     7381 gcccgcgcaa cggcgccggg tcgcgatcga gcaggcggat cagctcggca tggctgtcgg
     7441 gaacatccgc ttcgccgagc aggcgcttgt tgtagacgat caccgcgggc tcgtaggtgg
     7501 tgccgaaggc ctcgtccttc cacaccgccc agcgcggcag cgccgcggtc tcggcggagc
     7561 ggtgcggctg ggcgtggccg tcgttgacca gcttcacctg caggtccatc gccgagctcc
     7621 acaccacgtc ggcaccggcc agcccgcgcc gggcctcgtc gaccacgcgc gcatggagtt
     7681 cggccgagtg catgtcgtgg tactgcaccc gaacgccggg atggagggcc tcgaagtcgt
     7741 cgatgaccgg cttgaccacc tcgaggtcgg tcgccgcata gacgaccacc acgccctcca
     7801 ggcgtgcctg atcgaagcga tcggcgatcg gctcagcccg caccggggcg atcgccaccg
     7861 ccgccacgac cgccagcacc gtcttcagcg cggccctccc acccctgatc ctcatcaccc
     7921 ctcctcctcg tgctccgcgt gcccccgcgc ccgcaccgcc atgcggggaa tgcccggcat
     7981 gtgccgcctt ggacgcaccc cggctttgcg ccgataatcc cgcacacgcc tttcgattca
     8041 ctttcagcgg gaacaccgat gcgcctgttg ctggtcgagg accacgccga actcgccgaa
     8101 tgggtggcgc gctcgcttcg gcatgccggt ttcgtgctgg atgtgctcaa tcgtggcgac
     8161 catgccgatc agtccttgcg cacggaacgc tacgacctgg tgatcctcga tctgtccctg
     8221 ccggggctgg acggcctcga agtgctgcgc cgcctgcgca accgcgagag cggccccgac
     8281 aagcctgctc ccacaccggt gctggtgctc accgcgcgcg gcagcaccga ggagcgggtg
     8341 cacgggctca acctcggcgc cgacgattat ctgcccaaac ccttcgagct cgccgaactc
     8401 gaggcacgca tcaaggcgct gctgcgccgc gccggcaacc aggtgccgct ggtgcgcctg
     8461 ggccggctcg agttcgacac cactacccgc atggcggcgg tggacggaca ggcgctggca
     8521 ctgactccgc gcgagctggc cgtgctggaa gcgctgctcg cacgggtcgg tcgcccggtc
     8581 gcccgcgagg ccctgttcga gaaggtgttc gagttcgatg ccgacgcacg cgtggaggcg
     8641 atcgagatct acgtctcgcg cctgcgcaag aagctcgaag gcgcgggcgt ggcgatcaac
     8701 acgctgcgcg ggctcggcta cctgctcgcc gagaagccgg cagcctgaca tggacggcgc
     8761 ctccgggtcc ggaccggcaa acccgggcac ggacgatcgc gagcacggca cggctgccag
     8821 ggcggtagct gcgcccggac acggcggcag cctgcgtcag cgcgtgctcg tctggctgct
     8881 gccgcccctg gccgtgctgc tgctgatcaa cgccttcttc acccatcgca gcgcgctcga
     8941 cgcggtgaac caggcctacg accgcacgct taccgcctcg gtgcgcgcga ttggcgaacg
     9001 cacccactcg ctcggcggcc agatccaggt ggacatcccc tacgccgcct tcgaaggctt
     9061 cgaggccgat gccgccgagc gcatcttcca tgccgtattc gaccctgccg gcgggctggt
     9121 caccgggtat gccgacctgc gcccaccgga gctgcccgcg gacgacggca gcgtacgcat
     9181 cttcgacctc acccatgccg gcgagacggt gcgcctggcg gcgatgcgca agcgcctcta
     9241 cgaccccgcg ctgcgcggcg gcgacgcggt gctgatcgtg ttcgccgaga ccaccggcgc
     9301 gcgccgggcg ctcgcgcacg acctgttcgt cgccagcctg cagcgccagt cgctgctggt
     9361 cgcggtgggg gcgatcctgg tgctggccgc gctcggcacc gccttccgcc ccctgctgcg
     9421 cgtgcgcgac cacatgcgcg cgcgcgacga ggaggatctc accccggtgc gcatggaacg
     9481 cgtgccgagc gagatcgcgc cgctgatcga cgcgatcaac caccacatgg aacgcctttc
     9541 ggcaatgctc gccgcgcgcc ggcgcttcct cgccgacgcc gcgcaccaga tccgcacccc
     9601 gctcgcggtg ctcaccacgc aggccgaata cgggctgcgt cagaccgacc ccgaggagat
     9661 gctggcgacc ttcgccagcc tgctgcgcag cacccgcgcg gcgcgccggc tggccaacca
     9721 aatgctgacc ctgtcgcgcg ccgaagcggt caacggcctg atcggcgaac gcgccgcgct
     9781 cgacctcgcc ggcctggtga aggaggtggc gatggaactc gccccgctcg cgctgaagcg
     9841 cgacatcgac ctcgcctacg agggcatcgg acatgccctg acgatgcagg gcaacgcccc
     9901 gatgctgcgc gagatggtct ccaacctgat cgacaatgcc atccgctaca gcccgcctgg
     9961 ctcggcggtg accgtcgccg tcgccgcctt cggcgaggac gcggtggtga gcgtctgcga
    10021 ccagggaccc ggcatcccca ccgccgagcg cgagcgcgtg ttccagcgct tctaccgcat
    10081 cctcggccag ggcgacagcg aaggcagcgg gctggggctg gcgatcgtgc gcgagatcgc
    10141 gctcgcccac ggcggacggg tgacgctgcg cgacggtgcc ggcggccgcg ggctgtgcgt
    10201 ggaggtcgaa cttccgctcg gcagcacgcc aagcgcctga acgtcgctct tctcctcgtc
    10261 tccggatcgc gctgaaagcc gatcgaaagg cgtcggcgct gcaatgcgcg ccacgcgccc
    10321 gatccatccg gcgcgcacat ccgaacgatc gaggagactc ccatgcaacg cctttccgcg
    10381 ccggccctgg tggccgccgc cctcgccctg ccatcgctcg cgctggccca atcgaacgtc
    10441 accctctacg gcctgatcga cctcaacctc ggctacgaga aatccggctc gcagcgctac
    10501 gagggcatcg gccacggcga gctcaacggc tcgcggctcg gcttccgcgg cacggaggat
    10561 ctcggcaacg gcaacaaggc cctgttcgtg ctcgagagcg gcttcgaccc ctcgaccggc
    10621 gtggccgagc aaggcgggcg actgttcggc cgccagtcct tcgtcggcct ggagaacggc
    10681 ttcggccgcg tgaccctggg ccgccagtat tccccggcct tcgacgcgct cgatcccttc
    10741 gacgccacgg gcaacgccga ccgcagcgcc ggcctgctga tccgcaaggc gggcgcggtc
    10801 aagcccgcct acgagacgcg cttcgacaac atgatcaagg cacgctcggc gaaactcgcc
    10861 ggcttcgagc tcgagggcgg ctactggttc ggcaaggaga gcggaaccga ctcgaccgcg
    10921 cgccaggagg gcgacggcca tggcatcgcg atgctctacg ccaacggcgc gctcgccggc
    10981 tccctggtca cgcagaccgt gcagcgcgac gccagcggcg gcaaggtgcg caccgacggc
    11041 ttcgccctgt cctacgactt cggcatcgtg aagccctatt tcgcctggtc gcaggacaag
    11101 gagcgcggca gcgtgggcaa cggcaaggcg cgcagctggg acctcagcgc ggagatccgc
    11161 ctcgatccgg cgaacacggt ggcgatcagc tacgccgacc gcgacgaatc cgacggcgcc
    11221 gcgagcgagg acgccagcgg ctggtcggcc tattacctgc acgcgctgtc caagcgcacc
    11281 acgctgtacg ccggctactc gcagctcgac aacgaggtcg gcgccaacta cgcgatcggc
    11341 aacctgacgc cggccgccgg cgacaagcac caggtgatga tggcgggcat gcgccaccgc
    11401 ttctgatcca tccgccaggg cgacgctttc acgcagggca ggtgcacgcg gaaatcttcc
    11461 gctgcacctg ccttttgcgt cgacgcgcgg ccttctgcgc tacggtgcac ggcgacctcg
    11521 accgcagccg ccttgcgctg cggctgtctc cgcttcctgt ctccgcttcc tgtctccatt
    11581 ccctttccca atcagcgagc cctgccatga acccgcatga gaccccctcc caaccccgcg
    11641 gcgaacccgc catccgcacc atcgccatgc cggccgacgc caaccccgcc ggcgacatct
    11701 tcggcggctg gctgatggcc cagatggacc tcgccgccgg caacgtcgcc gcccgccgcg
    11761 cccgcagccg ctgcgcgacg gtctcggtgg accgcatcac cttcctgcgc ccggtgtata
    11821 tcggcgacga ggtcagcctg tacgccgaca tcgtgcgcgt cggacgcagc tcgatgacca
    11881 tccaggtcga ggcctggcgc cgctcgcgcg acggcgagga acaggtcaag gtcaccgacg
    11941 cgagcttcgt cttcgtcgcc ctcgaccagg gcggccggcc gcgggtgatc gaccccgtgt
    12001 ccggcccgca ggcggagcac tgagcgcgca ccctccggca caaaaaaaga gcgcccttgg
    12061 ggcgctcttc gaaaggcggc gggccgggag gggcccgccg cgctcgcgga ttccggtcat
    12121 cgcaggatga atcccggaat cccggaaggt gatcttcgcg ctcaggcggc caccgccttg
    12181 cgctgctcca tcatgcgcat caggcgcggg ccgagcagca tcgccgccgc cgccacccac
    12241 aggccgatcg agatcgggct ggcgaacagc acgccgacgt cgccatcgct gatcgacagg
    12301 gcgcggcgca ggttctgctc catcatgtcg ccgagcacga agccgaggat gaagggcgcg
    12361 atcgggaaat tcatcacccg cagcaggtag ccgacgccac cgatcagggt gccgaggatg
    12421 aggtcgaagg tcgtgccatg caccgcatac acgcccacca gcgagatcgc cgccacgccc
    12481 ggcaccagca gccagttggg gatcgagagg aacttcacga acacgcggat cagcgggatg
    12541 ttgagcacca gcagcatcac gttggcgatg aagagcgagg cgatgaggcc ccacacgatc
    12601 accggctggt cctggaacag ggtgggcccc ggcgtgatgt tgtacagcgt gagcgcgccg
    12661 atcatcaccg cggtggtgcc cgagcccggc acgcccaggg tcagcatcgg caccagcgag
    12721 ccgttggccg aggcgttgtt ggccgcctcc ggtgcggcca cgccgcggat gtcgccgttg
    12781 ccgaactggt ggtccttgcc cgccatgcgg cgctccatcg cgtagcacat cgccgaggcg
    12841 atggtggcgc cggcacccgg cagcacgccg atgaagaagc cggagaaggc cgagcgcagc
    12901 atcgtccact tcgccagcat cagctccttc atgttgaaca ggatgcggcc ggtgatggtg
    12961 atcggctcgc tcgcggtgtg ctcgttctgc agcatcagca tcagctcgct gaccgcaaac
    13021 aggccgatca cgatgacgat gaaggggatg ccgtcctgca ggtgcagcat gtcgaaggtg
    13081 tagcgataga cgccgctgtt ggcgtcgatg cccacggtcg acagcagcag gccgatcagg
    13141 caggcggtca tcgccttggg cagcgagtcg ccgaccaggc cgccgaggca ggacagcgcc
    13201 agcaccatca gcgcgaagta ctccgccggg ccgaaggcga tcgcccactc ggcgagcagc
    13261 ggcgcgaagg cgacgatgcc gatggtggcg agcagcgcgc cggtgaagga agcgagcgcc
    13321 gacatcgaca gcgcggggcc ggcgaggccc ttcttcgcca tcgggtagcc gtcgagcgtg
    13381 gtcatcaccg cgccggcgtc gccgggcacg ttgatcagga tggcggagat gcggccgcca
    13441 tactcgcaac ccgcatacac cgcgcacagc aggatcagcg cggactcggg cggcagcttc
    13501 atcgcgaacg cgatcggcag caggatggcg acgccgttga tcgggccaag gccgggcagc
    13561 aggccgacca cggtgccgat gaagcagccg atcagcgcga tcatcaggtt ttccggcgta
    13621 ccggcgacgg cgaagccgtc catcagaagc gacaaggttt ccatggttta ccccaggatg
    13681 ccgttcagcc acagcccggt gggcaatgcg acgtcgagca gacggtcgaa gaagaagaac
    13741 atgcccacgc ccagaccgat gccgccgacg acgccctgct tccaggtgcc gccgaagaag
    13801 cgcgccaccg ggacggtcat cagcgcggtc gcgatcgtga agccgagctt gtcgaagagc
    13861 agggcatagg cgagcacgat caggaacagc aggccgatgc gcttgagcac cgggggcggc
    13921 gcccacaccg tctcgctcgt cttctccagc atcagcgcga tcgcgcacag gccgagcagg
    13981 aacagcacca gcagcgggaa ggcgcgcggc cccaccggct cgtaggacat cggcgcctgc
    14041 aggtcccagc cgatccagat gccggccagg ctcaccagca gcagcacgcc gccgaagatt
    14101 ctttcactca tggtcgaact cccgtgtgcc gcctgctgcg gcgccccctc gggcgccggc
    14161 aggcgggcag ggtttgcgga ggaggttatt gcgcggtgag gccgagctcg acggcgagct
    14221 tcttgaagcg ctgcacttcc tgcttcacgt actcggtcgc cgccgcgccg gtgcgggcga
    14281 aggggaacag gccgcgctcg gcgcgcagct tgtcgaactc cggcgaggcc agcatcttgt
    14341 cgaaggtcgc cacccaccag gcgtagtccg catcggcgac cttgggaccg acgtagtagc
    14401 cgcgggcgat ggtccagtcg accttgtaac cctgctcgat cgcggtcggg atcgccgcca
    14461 gctcgcccgg caggcgctcg ggagcgagca ccgcgagcac gcgcacacgg ccgcccttca
    14521 ggtgcgccgc cacctcggag gcgtcgcccg agtaaacctg cacatggccg cccatcagcg
    14581 cggtcatcgc ctcgccaccg ccctcgaagg cgacgtagcg catcgacttg ggatccacgc
    14641 ccgcctcgcg cgcgaccagg gccgacttca tccagtcctg gctgccgacc gaaccgcccg
    14701 cgccgtacac caccttgccc gggttggcgc gggtggcctc gatcaggtcc ttgaggctct
    14761 ggaagggcga gtccgcgcgc acgatgatcg cgccgtagtc ggcacccacc gcggcgaccc
    14821 acttcacgtc atcctcggtg tacttgccga acttgccgat cgccaggttc aggaaggagc
    14881 cgccggagaa ggccaccacc gtgccggcct cggccgggcg gttggcgatc acggtgttgt
    14941 aggccaccgc gccgatgccg ccgggcatgt aggtgacgcg catcggcgcc tcgaggtagc
    15001 cgttctgcag cgcgctctgc acgagcttgc aggtgaggtc gaagccgccg ccgggcttgg
    15061 cgggcgcgat gcattcgggc ttctcggggg cggcatggac gacaccggcg gcgagcgtgg
    15121 caagcaccgc ggtggcggcg gcgaacaggg tcttcttgag cagcatggcg atctcctcca
    15181 tatcggaata tgttcgctgc gcggcggaca cgtgccgcct cgcagggtga ggcggacgat
    15241 acggaggagg gctttcgaat gcctttcagt gaagccgctc ggcagaagtg ccgggcaagc
    15301 aggatcacat cagcccgaca ccacgcagga gcgagacgcc gacgctggtg gtgaagagcg
    15361 agccgagcgt ggtcagcgcg atgatgttgg cggccagcgt ggcgttgccg cccatcgccc
    15421 gcaccatcga atagctcgcc gccgccgaag gcgaggacgc catcagcatc agcacgccga
    15481 gttccatggc gcggaatccc gccgcccatg cgccgagcgt gaacacgagc ggcatcgcca
    15541 ccagcttgcc gaaggtggcc agcgcggtgt tgccggcttc gctgcgcagg ctgcggaaat
    15601 ccagcgtcgc gccggtgcac agcagcgcga gcggcagcgt catgtcggcg atgtaggcgg
    15661 ccgacttcat cgccaccgcg ggcagcggga gggcgaaggc cgacaccggc agcgcgagca
    15721 ggatgccgag gatcagcggg ttggtggcga tgccgcgcag gaccttgccc gggccctggc
    15781 tgcggtgcag cgaacggctg agcgtgatca cgccgaggat gttcaccagg atggtgagca
    15841 cgccgacgta gagcgaggcc atcgccagcc ccgcctcgcc gtaggcgttg ccgcagtagg
    15901 ccaggccgac cacgccgaaa ttggagcgga aggcgccctg caccaccacg ccgcggtcgc
    15961 gctcgggctg cacgacgaag ctcgccaccc actccatcag cagccagatc gccaccgtcg
    16021 ccgccaggcc atacaccagc atgccggcgc tcgccgagtc ctccaggcgc gtgcgcgcga
    16081 tgttgacgaa cagcatggtc ggcaggccga ccacgaacac cagccgcgag cccgccgcga
    16141 tgaagccctc ggtgatcagc ccgacccgac gcagcagcac gccgaggccg agcagcgtga
    16201 agatcggccc ggtgaccccg agggagaagt cgagcagttc gaggaatccg gacatcaggg
    16261 ccgcaacacg aagagcagca cagacagcgt gcccaccgac accagggtcg acaggaacac
    16321 cgccgaggcc acggtggact ggcaggcgcc gtagcgctgc gcgaacagat agggcgtgat
    16381 gcccgccggc agcgccgcca gcagcacgcc ggtggccacc cacagcggcg gcacggcgaa
    16441 cacctgggtg gcgagcagcc acacgatcgc cggatgcacc accgccttca gcgccaccag
    16501 cagcgccggc tcgcgcagat tgccgcccag gctgtagcgc gtcagcgagg cgccgagcgc
    16561 gaacagcgcg cacggcgtcg ccgcgctgcc caggcccttg gccaccgcgt cgagcgggcc
    16621 ggggatgtcg atgccgagca gcgcgaacgc cagcccgcag gacaggcccc agatgatcgg
    16681 cgtgccgagc acgctcttcg tgagcttgag caacatcggc cccagcccct cgcgcgcgcc
    16741 ctgcgcggcc tcgatcagcg cggtggtggg cggcagcatc agcaggctgt ggcaggcgat
    16801 catcacgaac agcggcagcg ccgccggcgg gccgtaagcg gtcatcacca gcggaatgcc
    16861 gagcatcccg gtgttggaga agcccgcccc cagcccgagc accgcctgct cgccgagccg
    16921 gcgcccgaac agcacccgcc cgcccagcat cgccagcgca tacaccccga aggcgccgat
    16981 gtagtaggac agcagatagc cccagggcat cgcgtcgggc agctcggcct gcgcgatcgc
    17041 gcggaacagc atcaagggca gcgcgaagtt gaacacgaac acggacagcc cgcggttggc
    17101 cgcctcgtcg aacacgccgg agcgcgccgc gacgtagccg aggccaagcg tgccgaagac
    17161 cggcaggatg atgccgaaga tgatgtccat acaggccttc gtgcggggcg cgcgacggac
    17221 gcgctggaag gatcgggcgg ctctcgggag ccagatgcga acccgcatga tgcccgcaca
    17281 ccgtggcacc gtgcagtcgt agcaacacgg atgagggcgt tcccccgcct gcaccgcccc
    17341 ggcctgcgtc cggcctcgag tggagactac cggatcaggg tcgcgccagc ccgatgcgca
    17401 gcaccgccgc cacgttgcgc gcggtcagtt cgacgttggc cgcggcatcg tgcaaggcct
    17461 cgtccagcgt gcagggcttg cccagcacgc tgaacaccgc atcgatgccg tgcgcgtgca
    17521 ccacgccgac gtccgcgccg agcgagccgg cgataccgat caccgggatg ccgtggcgct
    17581 tggcgacgcg ggcgacgccg atcggggtct tgccatgcac ggtctggaag tcgatgcggc
    17641 cttcgccggt gatcaccagg tcggcgtcgc gcacgtgggc gtccagcccc acggcggcgg
    17701 tgacgatctc gatgcccggt ttcagcgtgg cgccgaagaa ggccagcatc gccgcgccca
    17761 tgccgccggc cgcaccggcg cccgctaccg cctcgaccgc gacgccgagg tcgcgctcga
    17821 cgatgcgggc gaagcgggcg agattggcat cgagcgcctg caccatctcg acgctggcac
    17881 ccttctgcgg gccgaacacc gccgaggcgc cgcgtggccc ggtgagcggg ttgtccacat
    17941 cgcaggcgac ctcgatgcgg cattgcgcca ggcgtggatc gagcgcggag acgtcgatgc
    18001 gctcgagggc ggcgagcgca ccgccgccgg ggccgagctc gccgccctgc gcgtcgagaa
    18061 ggcgggcgcc gagcgcctgc cccatgccgg cgtcgccgtc gttggtggcg ctgccgccga
    18121 tgccgacgat cagatgacgg gcccccgcat
//
LOCUS       DAOQNN010000020        17793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1190_length_17793_cov_9.971023, whole genome shotgun sequence.
ACCESSION   DAOQNN010000020 DAOQNN010000000
VERSION     DAOQNN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 17793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17793
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1190_length_17793_cov_9.971023"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="PKV97_02590"
     CDS             <1..99
                     /locus_tag="PKV97_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNB04793.1"
                     /translation="INKDPEAPIFQVADYGLVGDLFQVVPELTGAL"
     gene            121..786
                     /locus_tag="PKV97_02595"
     CDS             121..786
                     /locus_tag="PKV97_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNB04794.1"
                     /translation="MDLPAALLSAGIQGVVFALAALGLLALARKLPWRELAGGVHLNL
                     LLGFAVCLTLIWSMKAGVKPGLNLHLLGAMAATLALGPWKAIAALALALCGITLNGAL
                     EWGDWPANFVLMVLVPVSVASGLHRLIERLLPAHFFIFIFVTGFAGSALTAIAQGLTA
                     SSVLALSGAYSADFLWSDYLPFFLLLGFSEGWISGAIITLMVVYRPEWVSAFDDRRYL
                     LDK"
     gene            816..>2348
                     /locus_tag="PKV97_02600"
     CDS             816..>2348
                     /locus_tag="PKV97_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB04795.1"
                     /translation="MSQYNAPIRDMQFVMRELAGLDEVMQLPGNEEVSADLVDAILEE
                     ADKFASGVLAPLNWTGDQEGAKWNNGEVATAPGWKDAYTQFAESGWTALPCDPEYGGQ
                     GLPKLLATAVMEMWKSANMAFSLCPMLTSGAIEALMLRGTDEQKNLYLPKMIAGEWTG
                     TMNLTEPNAGSDLAAVRTKAEPQGDGTYKIFGQKIFITYGEHDLTDNIIHLVLARLPD
                     APEGVKGISLFVVPKFMVNADGSLGARNDVHCVSIEHKLGIHASPTAVLAFGDKGGAI
                     GTLVGEPNRGLEYMFIMMNEARFAVGMEGLALSERSYQHALQYAKDRIQGTDAGVRGG
                     PKVNILHHADVRRLLMNMKSKTEAMRALAYVVGAAFDKAHHHADEAVRAQNQAFVDLM
                     IPVVKGWCTENSIDVTSDGVQVHGGMGFIEETGAAQHFRDSRITTIYEGTTAIQANDL
                     IGRKIARENGATVGAVVKMMRAVEAEVAGVQDEAFAAIARALGKGIAAVEEAVAYILA
                     TYS"
     assembly_gap    2349..2448
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2449..2767
                     /locus_tag="PKV97_02605"
     CDS             <2449..2767
                     /locus_tag="PKV97_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNB04796.1"
                     /translation="KGIAAVEEAVAYILATYSQDIKAASVGSVPFLKLLGIVAGGWQM
                     ARAALVAKRKLEAGEGDAGFCKAKIVTARFYADHVLAQAGGLAYTVVNGAPGALELTE
                     ELF"
     gene            complement(2929..3711)
                     /locus_tag="PKV97_02610"
     CDS             complement(2929..3711)
                     /locus_tag="PKV97_02610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB04797.1"
                     /translation="MKHFPSRLAITLLAGFLALPALAASPVAKVNGVAIPGERSDAML
                     AEQRAQGAPDGEQLRNAVREELIRREVLSQEAGKRGLDKKADVKAQMEMARQAILIRA
                     YLQDYVRTNPVDDAELKKEYEAIKGRMGDKEYKPRHVLVETEDQAKAIIARLQGGTAF
                     EEVAKESRDPGSKERGGELGWSNPAMYVKPFSDAMVQLQKGKYTTTPVKSDFGYHVIQ
                     LDDVRDVQPPPFEEVKPQLQQRLQQQKIERHVLDLREKAKVE"
     gene            complement(3722..4009)
                     /locus_tag="PKV97_02615"
     CDS             complement(3722..4009)
                     /locus_tag="PKV97_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNB04798.1"
                     /translation="MSATRVERIRAALHERFAPTRLEIGDDSALHAGHAGAREGGGHY
                     RVFIVSEAFQGCSRVVRQRMIYDAVGDMMHKDIHALAIRALTAAEAESQGH"
     gene            complement(4006..4305)
                     /locus_tag="PKV97_02620"
     CDS             complement(4006..4305)
                     /locus_tag="PKV97_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNB04799.1"
                     /translation="MLYALIGEDAPGALETRLAVRPQHVARLEALRDDGRLLLAGPMP
                     AIDSPDPGPAGFAGSLIVAEFDSLASAEAWLRDDPYVRQGVFVRTTVRPFRKTLP"
     gene            complement(4305..4913)
                     /locus_tag="PKV97_02625"
     CDS             complement(4305..4913)
                     /locus_tag="PKV97_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HNB04800.1"
                     /translation="MKFLFDLLPVILFFAAYKIGGANPEASHAFAAGWLGDGIAVSQA
                     PILIATFVAILATFLQIALVWLKHRKVDTMLWISLAIIVVFGGATLFFHNPTFIKWKP
                     TALYWLFGVVLLGSASLLGRNLIRTMLEAQIKLPDPVWARLNLAWAGFFIAMGVLNLW
                     VAYNFSEEAWVDFKMFGGMGLMLAFVLAQGFYLSRHMEEESN"
     gene            complement(4910..6325)
                     /locus_tag="PKV97_02630"
     CDS             complement(4910..6325)
                     /locus_tag="PKV97_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB04801.1"
                     /translation="MAGTVEAAGGPARPRLLPSTLLWQTFLLVALLLIVALGSWVQIF
                     RYFQEPARSRDVAQMVVSVINLTRTALINADFDRRMDLLIDLAALEGIRIYPAEATDE
                     LAPLADTRPLRLLTADVRRQLGDHTRFASRWKTLDGFWVSFRLDPDDANDEYWVMLPP
                     ERIENPDDFGWLGWGGGALLAALLGAFLIVARVSAPLRQLARAARMVGSGERPPPQPE
                     SGPQEIALVARAFNQMAGNLARMDEDRALILAGVSHDLRTPLARLRLGIEMSGAPDDE
                     VTAMVADIGEMDRIIGQFLDFGRGDAQEPTAAIDLATLAREVTEPYRLRGVDLRLEVP
                     ESLFVQARALSIRRALANLIDNALRYAGGNAPLEVTVSIEDGQARIEVADRGPGIPES
                     EIERMRQPFTRLEKARSNTKGAGLGLAIVDRVMRAHQGRLELSARDGGGLRAALCLPL
                     AAPAGEQDRMRRSAHSEPDRP"
     gene            complement(6325..7098)
                     /gene="ompR"
                     /locus_tag="PKV97_02635"
     CDS             complement(6325..7098)
                     /gene="ompR"
                     /locus_tag="PKV97_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HNB04802.1"
                     /translation="MNQKNRYRILVVDDDARLRELLSRYLQEQGFSVKAVGDAPMMDR
                     ALHREHYDLIVLDLMLPGEDGLSICRRLRAAENQIPVIMLTAKGDDVDRIVGLEMGAD
                     DYVAKPFNPRELVARIQAVMRRQPHTLPGAPTPEDEIVAFGRVKVNLGTRVLVRDGEE
                     IQLTTGEFSLLKVLLTHPRQPLSRDKLMELARGREYGVFDRAIDVQVSRLRKLVEDDP
                     AKPRYIQTVWGFGYVFVPDEQKGGEDNGGQSDTPAGGED"
     gene            7426..7698
                     /locus_tag="PKV97_02640"
     CDS             7426..7698
                     /locus_tag="PKV97_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04803.1"
                     /translation="MLAFFVFAPTTASAQLRFIVGGEEQAQRLRPAGAEDRQEWRDTL
                     SDQRNEREGARERRRMSEEERSDLRRHMRDAARGAYREESGRKPQR"
     gene            7831..8523
                     /gene="tsaB"
                     /locus_tag="PKV97_02645"
     CDS             7831..8523
                     /gene="tsaB"
                     /locus_tag="PKV97_02645"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNB04804.1"
                     /translation="MNILSIETATEHGSVALLHGDELLVRRIQGAANHSEAVLRDLRE
                     LLAETGLAVARLDAVAFGAGPGAFTGLRLACGVAQGIALAADLGVAVVGSLQALALQV
                     GAPRVLAATDARMGEIYWASFVTDTEGVPQPQGMPRCCAPLDLELPPGEWSAAGSAFR
                     AWPEELEARTAGRLVACQPDLHPRAEEIARLGAIQAREGGLVAPELAAPLYVRDKVAF
                     TTAERLARGGRA"
     gene            8523..8969
                     /gene="rimI"
                     /locus_tag="PKV97_02650"
     CDS             8523..8969
                     /gene="rimI"
                     /locus_tag="PKV97_02650"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNB04805.1"
                     /translation="MLALAFAPMGEGDLDWVGAREAELHAHPWSRGNFADSLASGYDA
                     WILSCDGVPSGYAVVMNVLDEAHLLDIGIVGELQGRGLGKRFLDWLAEGAKTRGARDF
                     YLEVRPSNAAALRLYARSGFVEIGRRRGYYPAAGGREDAIVMRRAL"
     gene            8969..9883
                     /locus_tag="PKV97_02655"
     CDS             8969..9883
                     /locus_tag="PKV97_02655"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNB04806.1"
                     /translation="MSPRLPSRRDAVLREMGLGPIWRLRTRKAEVSEADDAEREAAEP
                     ASTETDATASPRVEVPRTPNPPPAAPAPAAGPRSSVAFQAGPHRAARPEPAAASTPAA
                     VRTAPAAPDPARGARISTLEWDALEAEIRDCKACGLCERRKQAVPGVGDRQARWMLVG
                     EAPGAEEDQRGEPFVGQAGRLLDNMLAAIGLKRGEGVYIANAVKCRPPHNRTPERGEI
                     AACQPYLDRQIALVQPQLLVALGRPAAQALLDREIAISAARGKRFERAGTPVVVTYHP
                     AYLLRNPQDKAKAWEDLCFARRLIAETG"
     gene            complement(9932..11182)
                     /gene="lplT"
                     /locus_tag="PKV97_02660"
     CDS             complement(9932..11182)
                     /gene="lplT"
                     /locus_tag="PKV97_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HNB04807.1"
                     /translation="MPLGFYIIMAAQFFSALADNALLIIAIAMLRDMAAPSEYEPLLK
                     LFFTVSYVALAAFVGAFADSMPKWRVMLISNTIKIGGCLMLFLGTHPLFAYAVIGLGA
                     AAYSPAKYGILTEYLPHRLLVVANGWIEGLTVAAIIVGTVIGGIMIRGDVSTWLMGLG
                     LPGAGSAFGAAVLVVGTLYLVAAIFNMYIPDTGVDHKPLKSNPLYLLHDFNHCLRLLW
                     RDKLGQISLAVTTLFWGAGATLQFIVIKWAEHNLGMNLSQASMLQGVVALGVATGAVL
                     AARYITLRRAVNVIPLGIAMGLGVMIMVGVTEAWVAMALMVIVGGLAGFFVVPMNALL
                     QHRGHILMGAGHSIAVQNFNENLSILMMTGTYALLIVLGLSINTVIVLFGLFVAGTML
                     LVKRRHEANQREFDNVALLEDRPH"
     gene            11400..12311
                     /locus_tag="PKV97_02665"
     CDS             11400..12311
                     /locus_tag="PKV97_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB04808.1"
                     /translation="MADRRLQVFHAVAKHGSFTRAAEHLHMTQPAVTFQIKQLEEHFD
                     TRLLDRGHGKITLTAAGELVLAYAERILGLSAELDSRVSELTDELAGQLRIGTSTTIA
                     AYWLPQVLESFKRRYPGVLPRVVVGTSKLTEDRVAARDLDLGLIEIATEQPSIERRSA
                     ARDDLLVICSPGHPLAKFKELTARDLVGHPFIDRDPGNGIRQIADEFFEAAGIAGSQI
                     TLCAELGSLAAVKQLAAAGLGFAIASGRAIRRDVEEGRLVAIPLQPRLHTPLEVILPR
                     DKFRSRLINAFADHVCEEFARIATEQD"
     gene            12373..13791
                     /gene="radA"
                     /locus_tag="PKV97_02670"
     CDS             12373..13791
                     /gene="radA"
                     /locus_tag="PKV97_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNB04809.1"
                     /translation="MARQKSAFVCTECGAQAVRWQGQCPQCQAWNTLVETVLEPAAPA
                     AGSRFAALAGETSRLQPLSALEPREEPRQPTGLDEFDRVLGGGLVAGGVVLIGGDPGI
                     GKSTLLLQALSNLAATSASAGSEGATGKGTKAGGVIYVSGEESGEQVALRAQRLQLPQ
                     SPLQMLAEINLERILHTLREARPRVAVIDSIQTVYSETLQSAPGSVAQVRECAAQLTR
                     FAKQSGTSLILVGHVTKDGTLAGPRVLEHIVDTVLYFEGDTHSSFRLIRAFKNRFGAV
                     NELGVFAMTERGLRGVSNPSAIFLSQHSQQVAGSCVLITQEGTRPLLVEVQALVDSSQ
                     SPNPRRLSVGLEQNRLAMLLAVMHRHAGVVCFDQDVFVNAVGGVKITEPAADLAILFA
                     IVSSLRDRPLRRGLAVFGEVGLAGEIRPAPRGQERLKEAAKLGFDTAIVPRANAPKHP
                     IEGLKVIAVERVEEALEQLRGL"
     gene            complement(14020..14463)
                     /locus_tag="PKV97_02675"
     CDS             complement(14020..14463)
                     /locus_tag="PKV97_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNB04810.1"
                     /translation="MSAPVLVFGWGNPSRGDDALGPLFVEAVEAMKLPGIECLTDFQL
                     QVEHALDLRRRSRILFVDASGTARPPFEFQPIAAARDDSFTTHSVSPQSVLQVFEDLE
                     GRPAPPSWLLAIRGDSWELGTAPAPSANANLRAAIAAFTAWLPDL"
     gene            complement(14460..15926)
                     /locus_tag="PKV97_02680"
     CDS             complement(14460..15926)
                     /locus_tag="PKV97_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNB04811.1"
                     /translation="MNFELETAPADAGTLRRVAIDPVSRVEGHGKVTILLDEDNKVHQ
                     VRLHIVEFRGFERFIQGRPYWEVPVMVQRLCGICPVSHHLAASKALDLVVGAARVTPT
                     ADKMRRLMHYGQILQSHALHFFHLSSPDLLFGFGSELGRRNIVGVAQQYPDIAKKGIL
                     LRKFGQEVIRLTAGKRVHGTGAVPGGVNRALSADERKLLLADAYQMIAWSRDAVRIIK
                     ELHQQDRALYDHFGTVRSSFMGLVAPDGSLDLYHGVLRAKDADGRILFDQVDYQTYDR
                     LITEEVKPWSYMKFPYFTSMGPEAGWYKVGPLARVQNCDSIPTPFAEHERRELVAYGG
                     GKPLHGSLAFHWARMIEMLFAAETIKDLLHDDDLSGTELMTSGERRREGVGVIEAPRG
                     TLFHHYQVGDDDLVTMANLIVSTTNNNQAMNTAVREVAKQYLHGQEITEGLLNHIEVA
                     IRAFDPCLSCATHALGQMPLEVTLVDAEGTELDRKYKA"
     gene            complement(16035..16613)
                     /locus_tag="PKV97_02685"
     CDS             complement(16035..16613)
                     /locus_tag="PKV97_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNB04812.1"
                     /translation="MDIDIAKIDAGGAPRKLKVATVSLAGCFGCHMSFLDIDERLLPL
                     LDLIEFDRSPLTDIKHCGPCDIGLIEGGVCNAENVHVLREFRANCKVLIALGACAVNG
                     GLPAQRNHLELADILQEVYRTGHGLAEGSRIPDDPELPLPLNQVHPVHEVVRIDHFLP
                     GCPPSGDAIWAFLTQLIEGRDPSLGHGLLHYD"
     gene            complement(16613..17344)
                     /locus_tag="PKV97_02690"
     CDS             complement(16613..17344)
                     /locus_tag="PKV97_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNB04813.1"
                     /translation="MNDSFLLDGEDIPFTPGQTVMQAAAAAGKYIPHLCWHPDFAAHG
                     SCKLCTVKIGGRFATSCTVRAAAGQEVENRTAELDAKRRTLLQLLFVEGNHFCPSCEK
                     SGDCALQATAYEMGMLTPHFDHFYPDRPVDASHPDVLLEFNRCILCELCVRASRDVDG
                     KNVFALAGRGTQSHLVVNSDTGRLADTDFAVTDRAAAVCPVGVILRKRVGFAVPIGQR
                     TYDRRPISASTSPEDKAGAGIEEAR"
     gene            complement(17341..>17793)
                     /locus_tag="PKV97_02695"
     CDS             complement(17341..>17793)
                     /locus_tag="PKV97_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNB04814.1"
                     /translation="HFFAHESCGFCTPCRVGTAVNARLLDKLAADHGSPYDLDEIAKM
                     HRLMQGASHCGLGNTATIAIDDMLAKFRPAFERRLHSPDYEPAFDLDAALSQARRMTG
                     RDDPGAHLGDNHEALVEERPHAQGATTAGAAQSVPSSAEAASAKDLQP"
BASE COUNT         2748 a         5968 c         6175 g         2802 t
ORIGIN      
        1 atcaacaagg atcccgaagc gccgatcttc caggtcgccg actacggcct cgtgggcgat
       61 ctgttccagg tggtgccgga actgaccggg gcgctctgac cgggcgcgag gagcgcgggc
      121 atggatctcc cggcggcgct gctttcggca ggcattcagg gagtggtgtt cgcgctcgcc
      181 gcgctgggac tgctcgcgct cgcgcgcaag ctgccgtgga gggagctcgc cggcggggtg
      241 catctgaacc tgctgctcgg cttcgcggtg tgcctcacgc tgatctggag catgaaggcc
      301 ggggtcaagc cggggcttaa cctgcacctg ctcggggcga tggcggcaac cctcgcgctc
      361 gggccgtgga aggcgatcgc cgcgctcgcg ctcgcgctgt gcgggatcac cctcaacggc
      421 gcgctggagt ggggcgactg gcccgccaac ttcgtgctca tggtgctggt tccggtttcc
      481 gtcgcgagcg gtctgcatcg cctgatcgag cgcctgctcc cggcgcattt cttcatcttc
      541 attttcgtga ccggctttgc cggttccgcg ctgaccgcaa tcgcacaggg gctgaccgcc
      601 tcgtccgtgc tggcgctgtc gggcgcgtat tccgcagatt tcctgtggag cgactacctg
      661 ccgttcttcc tgctgctcgg gttttccgag ggctggatca gcggcgcgat catcacgctg
      721 atggtggtgt atcggcccga atgggtgagt gcattcgacg atcgccgcta tctgctcgac
      781 aagtagggct gttcaacctt ttccggagcg caacaatgag tcaatacaac gcccccatcc
      841 gcgacatgca gttcgtgatg cgtgagctcg ccggtctcga cgaagtcatg cagttgcccg
      901 gcaacgagga ggtctcggcc gacctcgtgg acgcgatcct ggaagaagcc gacaagttcg
      961 ccagcggcgt gctcgctccg ctgaactgga ccggcgatca ggaaggcgcc aagtggaaca
     1021 atggcgaggt cgccaccgcg ccgggctgga aggacgccta cacgcagttc gccgagtcgg
     1081 gctggaccgc gctgccgtgc gatccggaat acggcggcca gggcctgccc aagctgctcg
     1141 ccaccgcggt catggaaatg tggaagtcgg ccaacatggc cttctcgctg tgcccgatgc
     1201 tgaccagcgg cgccatcgag gccctgatgc tgcgcggcac cgacgagcag aagaacctct
     1261 acctccccaa gatgatcgcc ggcgagtgga ccggcacgat gaacctgacc gagccgaacg
     1321 ccggctccga cctcgccgcg gtgcgcacca aggccgagcc gcagggcgac ggcacctaca
     1381 agatcttcgg gcagaagatc ttcatcacct acggcgagca cgacctcacc gacaacatca
     1441 tccacctcgt gctcgcccgt ctgccggacg cgcccgaggg cgtgaagggc atctcgctgt
     1501 tcgtcgtgcc caagttcatg gtcaacgccg acggcagcct gggcgcgcgc aacgacgtgc
     1561 attgcgtgtc gatcgagcac aagctcggca tccacgccag ccccaccgcc gtgctcgcct
     1621 tcggcgacaa gggcggcgcg atcggtaccc tggtcggcga gcccaaccgc ggcctcgagt
     1681 acatgttcat catgatgaac gaagcccgct tcgcggtcgg catggaaggc ctcgcgctgt
     1741 ccgagcgctc ctaccagcac gcgctgcagt acgccaagga tcgcatccag ggcaccgacg
     1801 ccggcgtgcg cggcggtccg aaggtcaaca tcctgcatca cgccgacgtg cgtcgcctgc
     1861 tcatgaacat gaagagcaag acagaggcca tgcgtgcact cgcctacgtc gtcggcgccg
     1921 ccttcgacaa ggcgcatcac cacgccgacg aggctgtgcg tgcgcagaat caggccttcg
     1981 tcgacctgat gatcccggtg gtcaagggct ggtgcaccga gaactcgatc gacgtgacct
     2041 cggacggcgt gcaggtgcac ggcgggatgg gcttcatcga ggagaccggc gcggcgcagc
     2101 acttccgcga ttcgcgcatc accacgatct acgagggcac gaccgccatc caggccaacg
     2161 atctgatcgg ccgcaagatc gcccgtgaga acggcgccac cgtcggtgcc gttgtgaaga
     2221 tgatgcgcgc ggtcgaggcc gaagtggccg gcgtgcagga cgaggccttc gcagccatcg
     2281 cgcgcgcgct cggcaagggc atcgcggccg tggaagaggc tgtggcctac atcctcgcca
     2341 cctactccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca agggcatcgc
     2461 ggccgtggaa gaggctgtgg cctacatcct cgccacctac tcccaggaca tcaaggcggc
     2521 ctcggtcggt tcggtgccct tcctgaagct gctcggcatc gtggctggcg gctggcagat
     2581 ggcgcgcgcc gcgctggtgg ccaagcgcaa gctcgaggcg ggcgagggcg atgccggctt
     2641 ctgcaaggcc aagatcgtca ccgcgcgctt ctatgccgac cacgtgctcg cgcaggccgg
     2701 cggtctggcc tacacggtcg tcaatggcgc cccgggcgcg ctggagctga ccgaagagct
     2761 gttctgaccg gatattgccc ggcgtggaag cgccggggcg accgagcgaa aaaaacccgc
     2821 tgccgggcaa gtgcccgcag cgggtttttc ttgtgttcgt gttcgcgcct tccagcgccg
     2881 cagcgccttc cacgcaggag cgtcggaagg cgcggacagg acgggcgctt actcgacctt
     2941 ggccttctcg cgcagatcga gcacgtggcg ctcgatcttc tgctgctgca ggcgttgctg
     3001 cagttgcggc ttgacctcct cgaacggcgg cggttgcacg tcgcgcacgt cgtcgagctg
     3061 gatcacgtga tagccgaaat cgctcttgac gggcgtcgtc gtgtatttgc ccttctggag
     3121 ctggaccatc gcatccgaga acggcttcac gtacatggcg gggttgctcc agccgagctc
     3181 gccgccgcgc tccttggagc ccgggtcgcg cgactccttg gcgacttcct cgaaggcggt
     3241 accgccctgc aggcgggcga tgatcgcctt ggcctggtct tcggtctcga ccagcacgtg
     3301 gcggggcttg tattccttgt cgcccatgcg gcccttgatg gcctcgtatt ccttcttcag
     3361 ctcggcgtcg tcgaccgggt tcgtgcgcac gtagtcctgc aggtaggcac ggatcaggat
     3421 ggcctggcgg gccatctcca tctgcgcctt cacgtccgcc ttcttgtcca ggcccctctt
     3481 gccggcttcc tggctcagta cctcgcggcg gatcagttcc tcgcgcaccg cattgcgcag
     3541 ctgttcaccg tcgggcgcgc cctgggcgcg ctgctcggcc agcatggcgt cggaacgctc
     3601 gccgggaatc gcgactccat tgaccttggc gaccggggat gcggcgaggg cgggcagcgc
     3661 gaggaagccg gcaagcaggg tgatggcgag acggctcggg aaatgtttca tggagattcc
     3721 ttcaatgtcc ttgtgattcg gcctctgcgg ccgttagcgc gcggatggcg agggcatgga
     3781 tgtccttgtg catcatgtcc ccgaccgcgt cgtagatcat acgctgccgg acgactctgc
     3841 tgcagccctg gaaagcttcc gagacgatga aaacccgata atgtccgcct ccctcgcgcg
     3901 cacccgcgtg cccggcgtgc agcgcgctgt cgtcgccgat ctcgagccgt gtcggtgcga
     3961 agcgttcgtg cagcgcggcg cggatgcgct cgaccctcgt cgcgctcacg gcagcgtctt
     4021 ccggaacggg cgcacggtgg tccgtacgaa cacgccctgg cgcacgtagg ggtcgtcccg
     4081 aagccaggcc tcggcgctgg cgagcgagtc gaactcggcg acgatcaggc tgccggcgaa
     4141 gcccgccggg ccggggtcgg gcgagtcgat cgcgggcatc ggcccggcca gcagcaggcg
     4201 gccgtcgtcg cgcaatgcct caaggcgggc gacatgttgc gggcgcaccg cgaggcgcgt
     4261 ctcgagcgcg ccgggagcgt cctctccgat cagggcgtag agcatcagtt gctttcctct
     4321 tccatgtgac gggacaggta gaaaccctgg gcgagcacga aggcgagcat cagccccatg
     4381 ccgccgaaca tcttgaagtc gacccaggct tcttcgctga agttgtaggc gacccaaagg
     4441 ttgagcacgc ccatcgcgat gaagaaaccg gcccaggcga ggttgagccg cgcccaaacc
     4501 gggtccggta gcttgatctg ggcctccagc atggtgcgga tgaggttgcg tccgagcagc
     4561 gaggccgagc cgagcagcac cacgccgaac agccagtaga gcgcggtcgg cttccacttg
     4621 atgaaggtgg ggttgtggaa gaagagggtg gcgccgccga atacgacgat gatcgccagg
     4681 ctgatccaca gcatggtgtc caccttgcgg tgcttgagcc acaccagtgc gatctgcagg
     4741 aaggtggcga ggatggcgac gaaggtcgcg atcaggatgg gcgcctgcga gaccgcgatg
     4801 ccgtcgccca gccagccggc agcgaaggca tgcgaggctt cggggttggc gccgccgatc
     4861 ttgtaggcgg cgaagaaaag gatcaccggc aggagatcga agaggaattt catggtcggt
     4921 ccggttcgct gtgcgcggag cgccgcattc tatcctgttc cccggcggga gccgcgagcg
     4981 gcaggcagag cgccgcgcgc agaccgccgc catcgcgggc ggacagctcg aggcggccct
     5041 ggtgggcgcg catcacccga tccacgatcg ccaggcccag gccggcgccc ttcgtgttgc
     5101 tgcgcgcctt ctcgagccgg gtgaagggct ggcgcatgcg ctcgatctcg ctctcgggga
     5161 tgcccggtcc gcggtcggcg acctcgatgc gtgcctgccc gtcctcgatg gagaccgtca
     5221 cctccagcgg cgcgttcccg cccgcgtagc gcagggcgtt gtcgatcagg ttggcgagcg
     5281 cgcgccggat cgacagggcg cgcgcctgta cgaacaggct ctccgggacc tccaggcgaa
     5341 gatccacgcc gcgcaaccgg tacggttcgg tgacctcgcg cgccagggtg gcgagatcga
     5401 tggccgcggt gggttcctgc gcgtcgccgc ggccgaagtc gaggaactgg ccgatgatgc
     5461 gatccatttc gccgatgtcc gcgaccatcg cggtgacctc gtcgtcgggc gcacccgaca
     5521 tctcgatacc caggcgcagg cgcgcgagcg gcgtgcgcag gtcgtgggaa acgccagcga
     5581 ggatcagcgc gcggtcctcg tccatgcgcg cgaggttgcc cgccatctgg ttgaaggcgc
     5641 gcgccaccag cgcgatctcc tgcgggccgc tctcgggctg cggaggcggg cgctcgccgc
     5701 tgcccaccat gcgcgccgcg cgcgcgagct ggcgcagggg cgcgctcacc cgggcgacga
     5761 tcaggaaggc gccgagcagg gcggcgagca gcgcgccccc cccccagccg agccagccga
     5821 aatcgtccgg gttctcgata cgctcgggcg gcagcatcac ccagtattca tcgttggcgt
     5881 cgtccggatc gagacggaag ctcacccaga agccgtccag cgtcttccag cgcgaggcga
     5941 agcgggtatg gtcgccgagc tgccgacgta cgtcggcagt gagcaagcgc aagggccggg
     6001 tgtcggcgag cggcgcgagc tcgtcggtgg cctcggcggg atagatgcgg atgccttcca
     6061 gggcggcgag gtcgatcagc agatccatgc gacggtcgaa gtcggcgttg atcagcgcgg
     6121 tgcgggtgag gttgatcacc gaaaccacca tctgcgccac gtcgcgcgag cgcgccggct
     6181 cctggaagta gcggaagatc tgcacccagc tgccgagggc gacgatcagc agcagcgcga
     6241 cgagcaggaa ggtctgccag agcagggtgc tcggcagcag gcggggccgc gccggcccgc
     6301 ccgctgcctc gaccgtgccc gccatcagtc ctcgccgccg gccggggtgt ccgactgccc
     6361 gccgttgtcc tcgccgccct tctgctcatc gggcacgaag acgtagccga aaccccacac
     6421 ggtctggatg tagcgcggct tggcgggatc gtcctcgacc agcttgcgca ggcgcgagac
     6481 ctggacgtcg atcgcccggt cgaacacacc gtactcgcgc ccgcgcgcga gttccatcaa
     6541 cttgtcgcgg gacagcggct ggcgcgggtg ggttagcagc accttgagca gcgagaattc
     6601 gccggtggtg agctgaatct cctcgccatc gcggacaagc acgcgggtgc ccaggttcac
     6661 cttgacccgg ccaaacgcga cgatctcgtc ctcgggggtg ggcgcgcccg gcagggtgtg
     6721 gggctggcga cgcatcaccg cctggatacg cgccaccagc tcacgcgggt tgaagggctt
     6781 cgcgacgtag tcgtcggcgc ccatctccag cccgacgatg cgatccacgt cgtcaccctt
     6841 ggcggtgagc atgatgaccg ggatctggtt ctcggccgcg cgcagccggc gacagatcga
     6901 cagcccgtcc tcgccgggca gcatcaggtc gagcacgatc aggtcgtaat gctcgcggtg
     6961 cagggcgcga tccatcatcg gcgcatcgcc gacggccttc accgaaaagc cttgttcctg
     7021 aaggtatcgg gacagcaact cgcgcaggcg cgcgtcgtca tccaccacga gaatgcgata
     7081 gcggtttttc tggttcatga acggaatgct agcctgactc gcggcagggt gccagccttc
     7141 ccggggccgg tcgcgggggg ctcccggtaa gccttcgtaa ggcggggacg gagaggcaat
     7201 atcggcttac aaagcagtcg cgatcaggcc cgcgaaggct ttgcgcaccg ggcggtttgc
     7261 cgtaaggtgc ggtcatcgct ttcctgcgtc ggttccgtgc ctcctcgagg ccgtaggggc
     7321 cgacgtcgtg ctctcatgac ccctttccgc gtttcccgcg ccttccggca tcccgcgcag
     7381 caccttgcct gccggccgtg cgtgcgcgct tccctcgttt gcggcttgct ggcgttcttc
     7441 gtcttcgcgc ccaccacggc cagcgcgcag ctacgcttca tcgtcggtgg agaggagcag
     7501 gcgcaacgct tgcgtccggc gggagccgag gaccggcagg aatggcgcga caccctgagc
     7561 gaccagcgca acgagcgcga gggcgcgcgc gagcgccggc gcatgagcga ggaggagcgc
     7621 agcgacctgc gccgccacat gcgcgacgcc gcgcgcggcg cctaccgcga ggaatcgggg
     7681 cgcaagccgc agcgctgagc acgcggcaat ccggcagtcg ggatacccgc ccccgcatgc
     7741 ggtcgcggtc ttcgttcgcc cctgtcgatg ccgccgcgtg acaaccgcgg tcgcaacgaa
     7801 ccggcgcgct cccggtagaa tggtggcccc atgaacatcc tctccatcga aaccgccacc
     7861 gaacacggca gcgtcgcgtt gctgcatggt gacgagctgc tcgtccgccg catccagggc
     7921 gcggccaatc actccgaggc ggtgctgcgc gacctgcgcg aactccttgc cgagaccggg
     7981 ctcgccgtgg cgcggctgga cgcggtcgcc ttcggcgccg gtcccggtgc cttcaccgga
     8041 ctgcggctgg cttgcggtgt cgcgcaaggg atcgcgctgg cagcggatct cggcgtggcc
     8101 gtggtcggca gcctgcaggc gctcgcgctc caggtcggcg cgccgcgggt gctggcggcc
     8161 accgatgcgc gcatgggcga gatctactgg gcgagcttcg tgaccgacac cgaaggggtg
     8221 ccgcagccgc agggcatgcc gcgctgttgc gcgccgctgg atctcgagct gccccccggc
     8281 gagtggagcg ctgccgggtc ggccttccgc gcctggccgg aggaactcga ggcgcgcact
     8341 gcagggcgtc tcgtcgcctg ccagcccgac cttcacccgc gtgcggaaga gattgcccgc
     8401 ctcggggcga tccaggcgcg cgaaggcggg ctggtggccc ccgagctggc cgcgccgctc
     8461 tacgtgcgcg acaaggtcgc cttcaccacc gccgagcgac tcgcgcgcgg agggcgtgcc
     8521 tgatgctggc gctggccttt gcgccgatgg gcgagggaga cctcgactgg gtcggcgcgc
     8581 gcgaggcgga gctccatgcc catccctggt cgcgcggcaa tttcgccgac tcgctggcca
     8641 gcggctacga cgcctggatc ctgtcctgcg acggtgtccc ttccggctac gcggtcgtga
     8701 tgaacgtgct cgacgaagcg catctgctcg acatcggcat cgtcggcgag ctgcagggca
     8761 gggggctggg caagcgcttc ctcgactggc tggccgaagg ggcgaagacg cgcggcgcgc
     8821 gggatttcta cctcgaggtg cggccttcca acgccgcggc gctgcgcctc tacgcgcgca
     8881 gcggcttcgt cgagatcggt cgccggcgcg ggtattaccc cgcggcgggt gggcgcgagg
     8941 acgcgatcgt gatgaggcgg gcgctgtgat gtcgccgcgc ctgccctccc gccgcgacgc
     9001 ggtgctgcgc gagatgggac tcggcccgat ctggcgcctg cgcacgcgga aggcggaggt
     9061 gtccgaagcg gatgatgcgg agcgtgaagc ggccgagcct gcatcgaccg aaaccgacgc
     9121 gaccgcctcg ccccgggtcg aggtgccgcg tacgccgaat cctcccccag ccgcaccggc
     9181 cccggctgcc ggaccgcgat cgagcgttgc gttccaagcc ggcccgcacc gtgccgcgcg
     9241 ccccgagccc gcggccgctt cgacgcccgc agccgttcgc actgcgcctg cggcgccgga
     9301 tccggctcgc ggcgcccgca tctccacgct cgaatgggac gcgctcgaag ccgagatccg
     9361 cgattgcaag gcctgtgggc tctgcgagcg ccgcaagcag gccgtgcccg gcgtgggcga
     9421 ccgccaggca cgctggatgc tggtgggcga ggcgccgggc gcggaggagg accagcgtgg
     9481 cgagcccttc gtcggccagg ccgggcgcct gctcgacaac atgctcgcgg cgatcgggct
     9541 gaagcgcgga gaaggcgtct acatcgcaaa tgcggtcaag tgccgaccgc cacacaaccg
     9601 cactccagaa cgcggcgaga tcgccgcctg ccagccgtat ctcgatcgcc agatcgcgct
     9661 cgtgcagccg cagctgctgg tcgcgctcgg ccgtccggcc gcccaggccc tgctcgatcg
     9721 cgagatcgca atctcggccg cgcgcggcaa gcgcttcgag cgcgcgggca ctccggtcgt
     9781 ggtcacctac catccggcct atctgctgcg caatccgcag gacaaggcca aggcatggga
     9841 agacctctgc ttcgcgcgcc ggctgatcgc cgagacgggg tgaaggcggg ggcagggcgc
     9901 cgggcgggcc gggggcgaag gccgcccggc ctcagtgcgg gcgatcctcg agcagcgcca
     9961 cgttgtcgaa ctcgcgctgg ttggcctcgt gccggcgctt caccagcagc atggtgccgg
    10021 cgacaaagag cccgaacagc acgatcacgg tgttgatcga caggccgagc acgatcagca
    10081 gcgcataggt gccggtcatc atcaggatcg acaggttctc gttgaagttc tgcaccgcga
    10141 tcgagtgccc ggcgcccatc aggatgtggc cgcggtgctg cagcagggcg ttcatcggca
    10201 ccacgaagaa gcctgccagc ccgcccacga tcaccatcag cgccatcgcg acccaggcct
    10261 cggtgacgcc gaccatgatc attactccga ggcccatggc gatgccgagc gggatcacgt
    10321 tcaccgcgcg ccgcagcgtg atgtagcgcg ccgccagcac cgcgccggtg gccacgccga
    10381 gcgccaccac gccctgcagc atcgaggcct gcgacaggtt catgcccagg ttgtgctcgg
    10441 cccacttgat cacgatgaac tgcagcgtcg cgcctgcgcc ccagaacagc gtggtgaccg
    10501 cgagcgagat ctgcccgagc ttgtcgcgcc agagcaggcg caggcagtgg ttgaagtcgt
    10561 gcagcaggta gagcggattg ctcttgagcg gcttgtggtc caccccggtg tcggggatgt
    10621 acatgttgaa gatcgccgcc accaggtaca gggtgccgac caccagcacc gccgcgccga
    10681 aggccgagcc ggcgccgggc aggcccagcc ccatcagcca ggtggagacg tcgccacgga
    10741 tcatgatccc gccgatcacg gtgccgacga tgatcgccgc caccgtgagg ccctcgatcc
    10801 agccgttggc caccaccagc aggcggtggg gcagatactc ggtaaggatg ccgtacttcg
    10861 ccggcgagta ggccgccgcg cccagcccga tcaccgcgta ggcgaagagt ggatgcgtac
    10921 ccaggaaaag catcaggcag ccgccgatct tgatcgtgtt gctgatcagc atcactcgcc
    10981 acttgggcat cgaatcggcg aaggcgccga cgaaggcggc gagcgcgacg taggagacgg
    11041 tgaagaagag cttgagcagc ggctcgtatt cggagggggc cgccatgtcc cggagcatgg
    11101 cgatggcgat gatcagcaag gcgttgtcgg ccagcgcgga gaagaactgc gctgccatga
    11161 tgatgtagaa gccgagcggc atcgtcgggt cgcggcggaa ggtcgaagcg cggtttatat
    11221 cacgaagcgg attgccctgt gatgatctcg cgcagcgctt gtgcgagggc gcgcagatgc
    11281 ccgtatggca acggtttgag gccgcagcga gcgggttctc gggatgaacg cgcagggatc
    11341 cacctgaatg cgccggtttg tgctttaatt cacgtaaata aaacgtactt atccacgcca
    11401 tggcagatcg cagactccag gttttccacg cggttgcgaa gcacggctcg ttcacccgag
    11461 ccgcggaaca cctccacatg acgcaaccgg ccgtcacctt ccagatcaag cagctcgagg
    11521 aacacttcga cacccgcctg ctcgatcgcg ggcacggcaa gatcacgctc accgccgcgg
    11581 gcgagctcgt gctcgcctac gccgagcgca tcctcggtct gtcggccgag ctcgactcgc
    11641 gcgtctccga actcaccgac gagctcgccg gccagctgcg catcggcacc agcacgacga
    11701 tcgccgccta ctggctgccg caggtgctgg agagcttcaa gcgccgctac cccggcgtgc
    11761 tgccgcgcgt ggtggtcgga acctccaagc tcaccgagga tcgcgtcgcc gcgcgcgatc
    11821 tcgacctcgg cctcatcgag atcgccaccg agcagcccag catcgaacgc cgcagcgccg
    11881 cgcgcgacga cctcctggtg atctgcagcc ccggccatcc gctggcgaag ttcaaggagc
    11941 tcaccgcccg cgacctggtc ggccacccct tcatcgaccg cgacccgggc aacggcatcc
    12001 gccagatcgc cgacgagttc ttcgaggccg ccggcatcgc cggcagccag atcaccctgt
    12061 gcgccgagct cggcagcctc gccgcggtca agcagctcgc ggccgcgggc ctcggcttcg
    12121 cgatcgcctc cgggcgcgcg atccggcgcg acgtggaaga gggcaggctg gtggcgatcc
    12181 cgctgcagcc gcggctgcac accccgctcg aggtcatcct gccacgcgac aagttccgct
    12241 cgcgcctgat caacgccttc gccgaccacg tctgcgagga gttcgcccgc atcgcgaccg
    12301 agcaggactg attccccgca agcttttgct ccggccgcgg ccggagccct gtcgcagtta
    12361 cggaagacct tgatggcacg gcagaaatcc gctttcgtct gcaccgaatg cggtgcgcag
    12421 gccgtgcgct ggcaaggcca gtgcccgcag tgccaggcct ggaacaccct tgtcgagacc
    12481 gtgctcgagc ccgcagcgcc ggctgcgggc agccgcttcg ccgcgctcgc cggcgagacc
    12541 agccggctgc agcccctgtc cgcgctcgaa ccccgcgaag aaccccgcca gcccaccggt
    12601 ctcgacgagt tcgaccgcgt gctcggtggc ggcctggtcg ccggcggcgt ggtgctgatc
    12661 ggcggcgatc cgggtatcgg aaaatccacc ctgctgctgc aggcgctctc caacctggcc
    12721 gcgacgagtg cgtcggctgg cagcgagggc gcgacgggca agggcaccaa ggcgggcggc
    12781 gtcatctacg tcagcggcga ggagtcgggc gagcaggtcg cgctgcgtgc ccagcgcctg
    12841 cagttaccgc agtcgcccct gcagatgctc gccgagatca acctcgaacg catcctccac
    12901 accctgcgcg aggcgcgccc gcgcgtggcg gtcatcgact cgatccagac cgtctattcc
    12961 gagaccctgc agtccgcccc cggctcggtc gcccaagtgc gcgaatgcgc cgcccagctc
    13021 acccgctttg ccaagcagag cggcaccagc ctgatcctgg tcggccacgt caccaaggac
    13081 ggcacgctcg ccggcccgcg cgtgctcgag cacatcgtag acaccgtgct gtacttcgag
    13141 ggcgacacgc actccagctt ccgcctgatc cgcgccttca agaaccgctt cggtgcggtc
    13201 aacgagctcg gcgtgttcgc catgaccgag cgcggcctgc gcggtgtgtc caatccctcg
    13261 gcgatcttcc tgtcgcagca ctcgcagcag gtcgccggca gctgcgtgct gatcacccag
    13321 gagggcacgc gcccgctgct ggtcgaggtg caggccttgg tcgattcgtc gcagagcccc
    13381 aacccgcgcc ggctgtcggt gggcctggag cagaaccgcc tggcgatgct gctcgcggtc
    13441 atgcaccgtc acgccggcgt ggtctgcttc gaccaggacg ttttcgtgaa tgcggtcggc
    13501 ggcgtcaaga tcaccgagcc ggccgccgac ctcgcgatcc tgttcgcgat cgtctcctcg
    13561 ctgcgcgacc gcccgcttcg gcgcggcctc gcggtgttcg gcgaggtcgg tctcgccggc
    13621 gagatccgcc ccgcaccgcg tggccaggag cgcctgaagg aagccgctaa gctcggcttc
    13681 gacaccgcca tcgttccgcg cgccaatgcg ccgaagcatc cgatcgaggg cctgaaggtg
    13741 atcgcggtcg agcgcgtgga ggaggccctg gagcagttgc gcgggttgta agcgggaagg
    13801 taagtcgcat ccttgtacga gcgccgccag gcgcgaatgc ggcgtcgacc gtcctaccca
    13861 cgtcgactgc ccgcacgcag gaatcgcggc tcgcgccgct cccacacgac acccgcaagc
    13921 gcgccgtttc ttgcaggagc gccgccaggc gcgaatgcgg cgtcggccgt cccacccacg
    13981 tcgataatga gtccgccgag ttcgcggctt gcgccgcttc tacaggtcgg ggagccaggc
    14041 cgtgaaggcc gcgatggcgg cgcgcaggtt ggcgttggcg gagggggctg gcgcggtgcc
    14101 gagctcccat gagtcaccgc ggatggcgag caaccaactg ggcggcgcgg gccggccctc
    14161 gaggtcctcg aacacctgta gcacgctctg cggcgagacc gagtgggtgg tgaagctgtc
    14221 gtcgcgggcc gctgcgatcg gctgaaattc gaaaggcggc cgtgccgtac cggatgcatc
    14281 gacgaacagg atccggctac ggcgacgcag atccagcgca tgctcgacct ggagctggaa
    14341 atcggtcagg cactcgatcc ccggcagctt catcgcctcg accgcctcca cgaacagcgg
    14401 cccgagcgcg tcgtcgccgc ggctcggatt tccccatccg aagaccagca ccggcgcgct
    14461 catgccttgt atttccggtc cagctccgtg ccctcggcat ccaccagcgt gacctccagc
    14521 ggcatctgcc cgagcgcgtg ggtggcgcag gacaggcagg ggtcgaaggc gcggatggcg
    14581 acctcgatgt ggttgagcag gccctcggtg atctcctgac cgtggaggta ttgcttcgcc
    14641 acctcgcgca ccgccgtgtt catcgcctgg ttgttgttgg tggtggagac gatcaggttg
    14701 gccatggtga cgaggtcgtc gtcgcccacc tggtagtggt ggaagagtgt gccgcgcggg
    14761 gcctcgatca cgcccacacc ctcgcgccgg cgctcgccgc tcgtcatgag ctcggtgccg
    14821 gagaggtcgt cgtcgtgcag caggtccttg atggtctcgg cggcgaagag catctcgatc
    14881 atgcgcgccc agtggaaggc gaggctgcca tgcagcggct tgccgccgcc gtaggcgacg
    14941 agctcgcggc gctcatgctc ggcaaacggc gtggggatac tgtcgcagtt ctgcacgcgc
    15001 gcgagcgggc cgaccttgta ccagcccgcc tccgggccca tcgacgtgaa gtagggaaac
    15061 ttcatgtagc tccagggctt gacctcctcg gtgatgaggc ggtcgtaagt ctggtagtcc
    15121 acctggtcga agaggatcct gccgtcggcg tccttggcgc gcagcacgcc gtggtagagg
    15181 tcgaggctgc cgtcgggcgc gaccaggccc atgaagctcg agcgcacggt gccgaagtgg
    15241 tcgtagagcg cgcggtcctg ctggtggagt tccttgatga tgcgcaccgc gtcgcggctc
    15301 caggcgatca tctggtaggc atcggcgagc aggagcttgc gttcgtcggc gctcagcgcg
    15361 cgattgacgc cccccggtac cgcgccggtg ccatgcacgc gcttgccggc ggtgaggcgg
    15421 atcacctcct ggccgaactt gcgcagcagg atgcctttct tcgcgatgtc ggggtattgc
    15481 tgtgccacgc cgacgatgtt gcgccgaccg agctcggagc cgaagccgaa cagcaggtcg
    15541 ggcgaggaca ggtggaagaa gtgcagcgcg tgcgactgca ggatctggcc gtagtgcatc
    15601 aggcggcgca tcttgtcggc ggtcggcgtg acgcgtgcgg cgccgacgac gaggtcgagc
    15661 gccttgctcg cggcgaggtg gtgcgacacc gggcagatgc cgcacaggcg ctgcaccatc
    15721 accggcactt cccagtacgg ccgcccctgg atgaagcgct cgaagccgcg gaactccacg
    15781 atgtgcaggc gcacctggtg caccttgttg tcctcgtcga gcaggatggt gaccttgccg
    15841 tggccttcca cgcgcgagac ggggtcgatt gcgacgcggc gcagggtacc ggcgtcggcg
    15901 ggggcggttt cgagttcgaa gttcatgtgg atatcgtcgt cagtgagagg ctcgcggctt
    15961 gcgccgctcc tacgaagaac ccaggcgctc gaactcctgg taggagcggc gcaagccgcg
    16021 agcctttcga ggtctcagtc atagtgcagc agcccatgtc ccaggctcgg gtccctgcct
    16081 tcgatcaact gggtcaggaa ggcccagatc gcgtcgcccg agggcgggca gccagggagg
    16141 aagtgatcga tcctcaccac ctcgtgcacc ggatggacct ggttcagcgg cagcggcagc
    16201 tcggggtcgt cgggaatgcg gctgccctcg gcgagaccgt ggccggttcg atagacctcc
    16261 tgcaggatgt cggcgagctc gagatgatta cgctgcgcgg gcaggccgcc attgaccgcg
    16321 cacgcgccga gcgcgatcag caccttgcag ttggcgcgga attcgcgcag cacatgcacg
    16381 ttctcggcgt tgcacacccc gccctcgatg aggccgatgt cgcagggacc gcagtgcttg
    16441 atgtcggtga gcggcgagcg gtcgaactcg atcagatcga gcagcggcag caggcgctcg
    16501 tcgatgtcga ggaaggacat gtggcagccg aagcagcccg cgagcgagac ggtggcgacc
    16561 ttgagcttgc gtggcgcgcc gccagcgtcg atctttgcga tgtcgatgtc catcatcgag
    16621 cctcctcgat gccggcaccg gccttgtctt cggggctggt gctcgcgctg atcggccggc
    16681 ggtcgtaggt ccgctgcccg atcggcacgg cgaagcccac ccgtttcctg aggatcacgc
    16741 ccaccggaca gaccgcggcg gcgcggtcgg tgacggcgaa gtcggtgtcg gcgagccgcc
    16801 ccgtgtcgct gttgaccacc aggtgcgact gcgtgccgcg gccggcgagc gcgaacacgt
    16861 tcttgccgtc gacatcgcgg ctggcgcgca cgcacagctc gcacaggatg cagcggttga
    16921 actcgagcag cacgtcgggg tgcgaggcgt ccaccgggcg atcggggtag aagtggtcga
    16981 aatgcggcgt cagcatgccc atctcgtagg cggtggcttg cagtgcgcag tcgccgctct
    17041 tctcgcacga agggcagaag tggttgcctt cgacgaagag cagctgcagc agcgtgcggc
    17101 gcttggcgtc gagctctgcc gtgcggttct ccacctcctg gccggcggcg gcgcgcaccg
    17161 tgcaggacgt ggcgaagcgc ccgccgatct tcaccgtgca cagcttgcac gagccgtgcg
    17221 cggcaaagtc ggggtgccag cacaggtgcg ggatgtactt gcccgcagcg gcggcggcct
    17281 gcatcacggt ctggccgggg gtgaagggga tgtcctcgcc gtcgagcagg aaggaatcgt
    17341 tcatggctgc agatcctttg ccgaggcggc ctcggcggag gaggggacgg actgcgcggc
    17401 gcccgctgtg gtggcgccct gcgcatgggg gcgctcctcg accagggcct cgtggttgtc
    17461 gccgaggtgg gcgccggggt cgtcgcggcc ggtcatccgg cgcgcctgcg acagcgccgc
    17521 gtcgaggtcg aaggcgggct cgtagtcggg cgagtgcagg cggcgctcga aggcggggcg
    17581 gaacttggcc agcatgtcgt cgatcgcgat cgtcgccgtg ttgcccaggc cgcagtggct
    17641 ggcgccctgc atcaggcggt gcatcttcgc gatctcgtcg aggtcgtagg gcgagccgtg
    17701 gtcggcggcg agcttgtcca gcaggcgggc gttgaccgcg gtgccgacgc ggcagggcgt
    17761 gcagaagccg cagctctcgt gggcgaagaa gtg
//
LOCUS       DAOQNN010000021        17720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1194_length_17720_cov_11.395641, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000021 DAOQNN010000000
VERSION     DAOQNN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 17720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17720
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1194_length_17720_cov_11.395641"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /gene="yhbY"
                     /locus_tag="PKV97_02700"
     CDS             complement(<1..418)
                     /gene="yhbY"
                     /locus_tag="PKV97_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HNB04815.1"
                     /translation="MTELSPAERSELRARAHHLNPVVTVAGNGLTPAVLAEIERALQA
                     HELIKVKVQGAEREQREALMQELCAAVDAAPVQHIGSILVVWRARREDDKSSSPAEKK
                     PVRASTAKSAAAFAAAARRAALAKASTESRRSPARAP"
     gene            501..1124
                     /locus_tag="PKV97_02705"
     CDS             501..1124
                     /locus_tag="PKV97_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HNB04816.1"
                     /translation="MKKNKTSRAWVHDHVNDPWVQRAQAEGYRARAAFKLLEIDERDH
                     LLRPGAVVVDLGAAPGSWCQLAVKRCGPKGRIFALDLLPMEPVAGVEFLLGDFTEDAV
                     LEEFEARLGGASVDVVLSDMAPNLSGVATVDQARSVHLCELALDFAVRHLKPGGQFVV
                     KVFQGEGFMEYRKQMEAAFVSVQVRKPKASRDRSAEVYLVGRGPRRP"
     gene            1247..3139
                     /gene="ftsH"
                     /locus_tag="PKV97_02710"
     CDS             1247..3139
                     /gene="ftsH"
                     /locus_tag="PKV97_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNB04817.1"
                     /translation="MNNLFKNLAIWMVIGVVLMTVFNQFNTRQTPASTMEYSQFLEEA
                     KAGRIARATVDGRVLKATTQEGRTITVYTPGVQDIWMISDLMRYGVQINASKPEEEQS
                     FLASVFVSWFPMLLLIGVWIFFMRQMQGGGKGGAFSFGKSKARMLDESANSVTFADVA
                     GCDEAKEEVAELVEFLRDPSKFQKLGGRIPKGVLMVGSPGTGKTLLAKAIAGEAKVPF
                     FSISGSDFVEMFVGVGAARVRDMFEQAKKQAPCIIFIDEIDAVGRQRGAGLGGGNDER
                     EQTLNQLLVEMDGFEGQTGVIVIAATNRPDVLDPALLRPGRFDRQVVVALPDIRGREQ
                     ILKVHMRKVPIAPDVDPQVLARGTPGFAGADLANLVNEAALFAARANKRLVDMEDFER
                     AKDKIMMGAERRSVVMPEEERRNTAYHESGHAVVARLLDKTDPVHKVTIIPRGRALGV
                     TMQLPTEDRYSQDRDRLLQTIAVLFGGRIAEEVFMKQMTTGASNDFARATDLARRMVT
                     QWGMSDTLGPMVYGEEEGEIFLGRQVTTHRNVSEATMQKVDAEIRRIIDQQYSLARRL
                     LEENSDKVEAMTAALLEWETIDAEQVNDIMAGRPPRPPKPASAPSSRPKDDAPGAAPT
                     APAPAA"
     gene            3240..4070
                     /gene="folP"
                     /locus_tag="PKV97_02715"
     CDS             3240..4070
                     /gene="folP"
                     /locus_tag="PKV97_02715"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNB04818.1"
                     /translation="MSVLQCGRFRLDLSQPRVMAIINLTDDSFSGDGLRDDLARALAR
                     AERALEEGAEMLDIGAESTRPGSDPVPEAQELERVVRFVEAARGWKVPLSIDTLKPTV
                     MRAALAAGADMINDINAFRASGAIEAVAAGESGLCVMHMLGQPRTMQQDPRYADVVGE
                     VTGFLDERVAALVAAGVARERIVLDPGFGFGKSVGHNYALLRELARVGVGGLPVLAGL
                     SRKSMLGAVTGRAVGERLAASVAGALIAVQNGARIVRVHDVAATRDALAVWKATVTGC
                     "
     gene            4127..5479
                     /gene="glmM"
                     /locus_tag="PKV97_02720"
     CDS             4127..5479
                     /gene="glmM"
                     /locus_tag="PKV97_02720"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNB04819.1"
                     /translation="MARKYFGTDGVRGKVGDTPITPEFVMRLGYAAGVTLVAREKLPA
                     GEHPAVLIGKDTRISGYMLEAALEAGFAAAGVDVMLAGPIPTPAVAYLTRALRLQAGV
                     VISASHNPFYDNGIKFFSADGSKLPDAVEAEIEAHLDEPMGCVDSARLGKARRIDDAA
                     GRYIEFCKSTFPNELDLRGLRIAVDCAHGAAYQIAPKVFHELGAEIIPVGVEPNGLNI
                     NDGVGATRPENLRQAVLKHGADLGIALDGDADRLIMVDHRGEIYDGDKLLYVVACARH
                     AEGRLEGVVGTLMSNLGFEHAIQRLGTPFARAKVGDRYVLELLQERGWKIGGENSGHI
                     ICLDRHTTGDGIVSALQVLAALKQRGLSLADACADLVFYPQRLINVRMPAGFDWRADG
                     SIASAQARAEQTLGDSGRVLLRPSGTEPLLRVMVEGRDGELVERLAREIAGVVEEAIA
                     "
     gene            5632..5872
                     /locus_tag="PKV97_02725"
                     /pseudo
     CDS             5632..5872
                     /locus_tag="PKV97_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765126.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
     gene            5995..6732
                     /gene="tpiA"
                     /locus_tag="PKV97_02730"
     CDS             5995..6732
                     /gene="tpiA"
                     /locus_tag="PKV97_02730"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNB04820.1"
                     /translation="MTRLVAGNWKMNGSLAANRELLGGLVSIPGVEIAVCVPYPYLAQ
                     AQQRPEGVALGAQDVSEYAVGAYTGEVSAAMLAEFGCRYAIVGHSERRALFGEEDATV
                     GRKALAALQAGLVPIVCVGETLAERDEARAVEVIGRQLEAVRAVLGAAAMGAVVLAYE
                     PVWAIGTGRSASVAQVAEVHAAIRAWLKVHGVAAGGVRILYGGSVKADNAAALFAVED
                     VDGGLIGGASLVAADFLAICRAAAGGV"
     gene            6765..7115
                     /gene="secG"
                     /locus_tag="PKV97_02735"
     CDS             6765..7115
                     /gene="secG"
                     /locus_tag="PKV97_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNB04821.1"
                     /translation="MGEYLFSLVLTVHVLVGLGVIGLVLVQHGKGADMGAAFGSGASG
                     SLFGASGSANFLSRTTAVLATVFFLTSLGLSFLASNKPAAPGSVMEGAQTAPATNPAP
                     AAPVESSPAQSIPK"
     gene            7181..7265
                     /locus_tag="PKV97_02740"
     tRNA            7181..7265
                     /locus_tag="PKV97_02740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7215..7217,aa:Leu,seq:gag)
     gene            7373..7747
                     /gene="ndhC"
                     /locus_tag="PKV97_02745"
     CDS             7373..7747
                     /gene="ndhC"
                     /locus_tag="PKV97_02745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNB04822.1"
                     /translation="MLENYFPVLMFMLVGLGFGLAPVILGRLLAPYRPDSEKNSPYEC
                     GFEAFEDARMKFDVRYYLIAILFILFDLEIAFLFPWATVFQEFIAAGEIAWFVFGSVM
                     VFLAILVIGYIVEWKNGALDWE"
     gene            7751..8227
                     /locus_tag="PKV97_02750"
     CDS             7751..8227
                     /locus_tag="PKV97_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNB04823.1"
                     /translation="MSIEGVFREGFVTTSLDAVINWTRTGSLWPMTFGLACCAVEMIH
                     AGCSRYDLDRFGVVFRPSPRQSDLMIVAGTLCNKMAPALRKVYDQMSEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGILQLQNKIKRTNTIAR"
     gene            8242..8847
                     /locus_tag="PKV97_02755"
     CDS             8242..8847
                     /locus_tag="PKV97_02755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNB04824.1"
                     /translation="MSAKLERLSQTLRDVFGNRIQSLVLDRGEVTIEVSAAEYPRVAR
                     QLRDHADLRFEQLIDLSGLDYSAYGNAIWEGKRFASVAHLTSVKHNWRLRVRVFADDD
                     AFPVIDSVVDVWPSANWYERESFDLYGILYSGHPDLRRILTDYGFVGHPFRKDFPVSG
                     YVEMRYDPEQGRVVYQPVSIEPRENTPRIVREEKYGDVGHG"
     gene            8840..10093
                     /locus_tag="PKV97_02760"
     CDS             8840..10093
                     /locus_tag="PKV97_02760"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNB04825.1"
                     /translation="MAEIRNYTINFGPQHPSAHGVLRLVLELDGEVVERADPHIGLLH
                     RGTEKLAETRTWVQSVPYMDRLDYVSMMCNEHAYCMAIEKLLGVEVPLRAQYIRVMFD
                     EITRVLNHLLNIGTHALDIGAMTMVLYTFREREDLMDVYEAVSGARMHAAYYRPGGVY
                     RDLPDRMPKYEVNKFKNEKTVRELNANREGSMLDFLEDFTNRFPTYCDEYETLLTDNR
                     IWKQRTVGIGVVSPEQALAWGFTGPMLRGSGIAWDLRKKQPYEVYDRVDFDVPIGKNG
                     DCYDRYLCRMEEMRQSNRIIKQCIDWLRKNPGPVITDNHKVAPPSREAMKTNMEELIH
                     HFKLFTEGMHVPKGEVYAAVEHPKGEFGVYAVSDGANKPYRLKLRAPGFAHLAAMDDI
                     ARGHMIADVVAIIGTMDVVFGEIDR"
     gene            10182..10661
                     /gene="nuoE"
                     /locus_tag="PKV97_02765"
     CDS             10182..10661
                     /gene="nuoE"
                     /locus_tag="PKV97_02765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNB04826.1"
                     /translation="MLSQESLQQIDREIAKYPPDQTQSAVMAALRIAQVEKGWLPKEL
                     IEFVARYLGMPPIAAFEVASFYNMYDLAPVGRHKITVCTNLPCALSGGVHAADYIKEK
                     LGIDFNETTPDGKFTLKEGECMGACGDAPVLLVNNHHMCSWMTTEKIDQMLAELDKK"
     gene            10673..11977
                     /gene="nuoF"
                     /locus_tag="PKV97_02770"
     CDS             10673..11977
                     /gene="nuoF"
                     /locus_tag="PKV97_02770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNB04827.1"
                     /translation="MILAGCDGDRTWRLQDYVARGGYSALRKILAEKIPQDAIIAELK
                     TSVLRGRGGAGFPTGLKWSFMPRSFPGDKYLACNSDEGEPGTFKDRDILRYNPHSVIE
                     GMAIAAYAMGAARGYNYIHGEIFETYSRFEEALAEAREAGFIGRNILGSDFSFELFAH
                     HGYGAYICGEETALLESIEGKKGQPRFKPPFPASYGLYGKPTTINNTETFASVPFIMN
                     MGGEAFLNLGKPNNGGMKLFSVSGHVNRPGNYEIPLGTPFAELLEMAGGMRGGRKIKA
                     VIPGGSSAPVVPGGVMMDCTMDYDSISKAGSMLGSGAVIVMDETTCMVKALERLSYFY
                     FEESCGQCTPCREGTGWLYRVVHRIEHGLGRPDDLDLLNSVTTNIMGRTICALGDAAS
                     MPVQSFVKHFGSEFEYHIENKKCLVPPEVQRAGSQIYVSPSC"
     gene            11971..14310
                     /gene="nuoG"
                     /locus_tag="PKV97_02775"
     CDS             11971..14310
                     /gene="nuoG"
                     /locus_tag="PKV97_02775"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNB04828.1"
                     /translation="MLEIEIDGKQVQVNDGSTVIEAAAVAGSYIPHFCYHKKLSIAAS
                     CRMCLVQVEKAPKPLPACATPVTNGMKVWTHSELAVKAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGSTASRYQEEKRVVFNKNLGALVSTDMTRCINCTRCVRFTTEIA
                     GEMELGQAFRGEHAEIMPFIEKTVDSELSGNIIDLCPVGALTSKPFRFAARTWELSRR
                     KSVSPHDSLGANLIVQTKHDVVKRVLPFENEDVNECWLSDKDRFSYEALSSEQRLGAP
                     MIRQGGQWKQVEWQAALEFVATGLRGVERDHGAASIGALASPHATVEELHLLQKLMRG
                     LGSENIDFRLRQVDFRADGKRAGAPWLGMPIAGVKDLTRLLVVGSFLRKDAPLLAQRV
                     RQAAKKGLHVSAIGPNAEEWLLPVRNRALVAPSAMVATLGQVVVALAKEKGAEVSVAL
                     LGKLPETVSDEALAIAQSLASGRKVAVWLGNLAVQHARASELQMLAQEIARLTDGSFG
                     FIGEAANSVGGYLAGAVPGAGGLNAAAMIEQPRAAYVTLNLEPDLDLANPAAAMAALD
                     GAQLVVALTSFDSPSLRQVADVMLPIAPFTETSGTFVNCEGRAQSFTAVAKPFADVRP
                     GWKVLRVLGNLLALEGFAHADSEAVRAEALAGDLASRLDNGVEGIVVSSATGPAGVLE
                     RVADVPIHFADPIARRAPSLQKTRDAAAPTARIAPATLAALGLAAGDRVRVAQGTASV
                     ELVAQADDGLAAGCVRVAAAHPSTAALGAMSGDLSVERA"
     gene            14310..15359
                     /gene="nuoH"
                     /locus_tag="PKV97_02780"
     CDS             14310..15359
                     /gene="nuoH"
                     /locus_tag="PKV97_02780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNB04829.1"
                     /translation="MEAMLQPVADFFGPSWTAIWTLLKIVAIIAPLMICVAYLTLAER
                     KVIGYMQVRIGPNRVGPKGLLQPIADGMKLLFKEIIVPSGASKGLFILGPILAIAPSL
                     AAWAVVPFDDGLVLADVNAGLLFLLAITSMEVYGVIVAGWASNSKYPFLGSMRAAAQM
                     VSYEVSMGFALICVLLISASLNLTDIVMSQNEGRFADMGLGVLSWNWIPLFPMFVVYV
                     ISGIAETNRAPFDVVEGEAEVVAGHMVEYSGMTFALFFLAEYANMILVSILTSILFLG
                     GWLSPVGFLPDGFHWLAVKTAFILLLFLWARATFPRFRYDHIMRLGWKVFIPVTLVWL
                     FVVTVWMMSPLSIWS"
     gene            15367..15852
                     /gene="nuoI"
                     /locus_tag="PKV97_02785"
     CDS             15367..15852
                     /gene="nuoI"
                     /locus_tag="PKV97_02785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNB04830.1"
                     /translation="MGAKDYIGSLFLKELVKGMALTGRHFFQRKITILFPEEKTPQSN
                     RFRGLHALRRYPNGEERCIACKLCEAICPAMAITIESDQRDDGSRRTTRYDIDLTKCI
                     FCGFCEEACPVDAIVETRVLEYHGEQRGDLYYTKQMLLAVGDRHESQIAADRQQDAKY
                     R"
     gene            15886..16491
                     /locus_tag="PKV97_02790"
     CDS             15886..16491
                     /locus_tag="PKV97_02790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNB04831.1"
                     /translation="MEFKTFVFYFLAAIMVLAALRVITARNPVHAALFLVLTFFNAGG
                     LWLLLQAEFLAVTLVMVYVGAVMVLFLFVVMMLDINLDRLREGFWSYLPVGALVGILM
                     LVEMVMVLGGGYFGLEAMPAPAPVAADYSNTRELGRVLYTDYVYAFELASLVLLVAMV
                     SAVALTLRKRKGLKSIPPSEQVAVRREGRVELVKMQAEKDD"
     gene            16555..16860
                     /gene="nuoK"
                     /locus_tag="PKV97_02795"
     CDS             16555..16860
                     /gene="nuoK"
                     /locus_tag="PKV97_02795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNB04832.1"
                     /translation="MLSLSHFLILGAILFAISVVGIFLNRKNLIVLLMAIELMLLSVN
                     MNFVAFSHYLGDIAGQVFVFFILTVAAAESAIGLAILIVMFRNMRTIHVDDLDSLKG"
     gene            16873..>17720
                     /locus_tag="PKV97_02800"
     CDS             16873..>17720
                     /locus_tag="PKV97_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNB04833.1"
                     /translation="MSDMKAIYLLVPLAPLAGAILAGLFGKQIGRAGAHVVTILGVAI
                     AFVASLFIYQDVAAGNTFNGTVYTWMQAGGIDFEVGFLIDSLTVMMMLVVTFVSLMVH
                     IYTIGYMSEDPGYQRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLLIGFWY
                     ERPTAIYANMKAFLVNRVGDFGFLLGIGLVAAYAGSLDYAEVFAKAKELSVTEMAVTG
                     WPLLTAICICLFIGAMGKSAQVPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVARM
                     SPLFELS"
BASE COUNT         2940 a         5673 c         5897 g         3210 t
ORIGIN      
        1 cgggcgcacg tgccggcgag cggcgcgact ccgtgctcgc cttggcgagc gcggcacgac
       61 gcgcggcggc ggcgaaggcg gcggccgact tggcggtgga agcgcgcacc ggcttcttct
      121 ctgcgggcga ggagctcttg tcgtcctcgc ggcgcgcacg ccagacgacg aggatgctgc
      181 cgatgtgctg caccggggcg gcgtccaccg cggcgcacag ctcctgcatc aaggcctcgc
      241 gctgctcgcg ttcggcgccc tggaccttga ccttgatgag ctcgtgggcc tgcagggcgc
      301 gttcgatctc ggcgaggacg gccggcgtga ggccgttgcc cgctacggtc acgacggggt
      361 tcaggtggtg ggcacgggcg cgcagctcgc tgcgctcggc cggggaaagc tcggtcattg
      421 caaatctctg gatgggtaaa gcccgggacg cggattctac cccgtacgtg ccaaggatgg
      481 ccgtcgcgga tggagtgagg atgaagaaga acaagaccag ccgtgcctgg gtgcacgatc
      541 atgtgaacga tccctgggtg caacgcgccc aggccgaggg ttatcgcgca cgcgccgcct
      601 tcaagctgct cgagatcgac gagcgcgacc acctgctgcg tccgggcgcg gtggtcgtcg
      661 acctgggagc ggcgcccggc tcgtggtgcc agcttgcggt gaagcgctgc gggcccaagg
      721 gacgcatctt cgcgctcgac ctcctgccca tggagccggt ggcgggggtg gagttcctgc
      781 tcggcgactt taccgaggat gccgtgctcg aggagttcga ggcgcgcctt ggtggcgcga
      841 gcgtggatgt ggtcctgtcg gatatggcgc cgaacctttc aggggttgcg acggtcgacc
      901 aggcacgttc ggtacatctt tgcgaattgg cgctggactt cgcggtgcgt catctcaagc
      961 cgggtggaca gttcgtggtc aaggtgttcc agggcgaggg cttcatggag tatcgcaagc
     1021 agatggaggc ggcgttcgtc tcggtgcagg tgcgcaagcc caaggcttcg cgcgaccgta
     1081 gcgccgaggt ctatctcgtc ggccgggggc cgcgccggcc ttgagttcgc atgcgggggt
     1141 ggcgattgag ttgcgctatg taccccattg gtttgcccgt gggaacaatc gccactagaa
     1201 tcggtcagtc gcgtacaggc cggaatggct ttcggggaac agaaagttga acaatctctt
     1261 caagaatctc gcgatctgga tggttatcgg cgtcgtgctg atgacggtat tcaaccagtt
     1321 caacacgcgc cagacgccgg ccagcaccat ggagtattcc cagttcctgg aggaggccaa
     1381 ggcgggacgg atcgcgcgcg cgaccgtcga tggccgcgtg ctcaaggcca ccacccagga
     1441 agggcgcacg atcacggtgt atacgccggg cgtgcaggac atctggatga tttcagacct
     1501 gatgcgctac ggcgttcaga tcaacgccag caagcccgag gaggagcagt ccttcctcgc
     1561 cagcgtattc gtgtcgtggt tcccgatgtt gctgctgatc ggcgtgtgga tcttcttcat
     1621 gcgccagatg cagggcgggg gcaagggcgg ggcgttctcc ttcggcaaga gcaaggcgcg
     1681 catgctcgac gagtcggcca attcggtgac ctttgccgac gttgccggct gcgacgaggc
     1741 caaggaagaa gttgccgagc tggtcgagtt cctgcgtgac ccgtccaagt ttcagaagct
     1801 cggcggccgc attcccaagg gcgtgctgat ggtcggttcg ccgggtaccg gcaagaccct
     1861 gctcgccaag gcgatcgccg gagaggccaa ggtgcccttc ttctcgatct ccggctccga
     1921 cttcgtcgag atgttcgtcg gcgtaggcgc ggcgcgcgtg cgcgacatgt tcgagcaggc
     1981 caagaagcag gcgccctgca tcatcttcat cgacgagatc gacgcggtcg gccgccagcg
     2041 tggtgcgggt ctgggcggcg gcaatgacga gcgcgagcag acgctcaacc agttgctggt
     2101 cgaaatggac ggtttcgagg gccagaccgg cgtgatcgtg atcgccgcga ccaaccgccc
     2161 cgacgtgctc gacccggctc tgctgcgccc gggccgcttc gaccgccagg tggtggtggc
     2221 gctgcccgac atccgtggtc gcgagcagat tctcaaggtg cacatgcgca aggtgccgat
     2281 cgcgcccgac gtcgatccgc aggtgctggc gcgcggcacg ccgggctttg ccggtgccga
     2341 cctcgccaac ctggtgaacg aggctgcgct gttcgccgcg cgcgccaaca agcgtctggt
     2401 cgacatggag gacttcgagc gcgccaagga caagatcatg atgggcgccg agcgccgctc
     2461 ggtggtcatg cccgaggagg agcgccgcaa caccgcctac cacgagtccg gtcacgcggt
     2521 ggtggcgcgt ctgctcgaca agaccgatcc ggtgcataag gtgaccatca tcccgcgcgg
     2581 ccgtgcgctg ggtgtgacca tgcagctgcc gaccgaggat cgctacagcc aggaccgcga
     2641 tcgcctgctg cagaccatcg ccgtgctgtt cggtggccgc atcgccgaag aggtcttcat
     2701 gaagcagatg acgaccggtg cgtcgaacga cttcgcccgt gccactgacc tcgcgcgccg
     2761 gatggtgacg cagtggggca tgtccgacac gctcggaccg atggtgtatg gcgaggagga
     2821 gggcgagatc ttcctcggcc gccaagtcac cacgcaccgc aacgtgtccg aggcgacgat
     2881 gcagaaggtc gacgcggaga tccgccgcat catcgatcag cagtactcgc tggcgcggcg
     2941 cttgctcgaa gagaacagcg acaaggtcga ggcgatgacg gccgcgttgc tggaatggga
     3001 gaccatcgac gccgagcagg tgaatgacat catggccggc cgccctccgc gtcccccgaa
     3061 gccggcatcg gcgccgtcca gccgtccgaa ggacgacgcg ccgggtgcgg cgcccaccgc
     3121 gccagcacct gccgcctgag ttcgacaact tccgtttcaa ggggccggcc ggaccggccc
     3181 ctttccattc cggcgtccgc gcgcctgcgc ccggacgcct tgcttttccc gaggtctcga
     3241 tgtccgtcct gcaatgcggc cgcttccggc tcgatctgtc ccagcccagg gtgatggcaa
     3301 tcatcaacct gaccgacgac tccttctccg gcgacggtct gcgtgacgac ctcgcccgtg
     3361 cgctcgcgcg cgccgagcgt gcgctggagg aaggggcgga gatgctcgat atcggcgccg
     3421 agtcgacccg gccgggatcg gacccggtgc ccgaggcgca ggagctcgag cgcgtggtgc
     3481 gcttcgtcga ggccgcgcgc ggctggaaag tgccgctctc gatcgacacc ctgaagccga
     3541 cggtgatgcg cgccgcactc gcggcgggtg cggacatgat caacgacatc aacgcgttcc
     3601 gcgcatcggg tgcgatcgag gcggtcgccg cgggcgagtc cggcttgtgc gtgatgcaca
     3661 tgctcggcca gccgcgtacg atgcagcagg atccgcgcta cgccgacgtg gtcggcgagg
     3721 tgaccggctt cctcgacgag cgcgttgccg ccctcgtggc cgcaggcgtg gcgcgcgagc
     3781 gcatcgtgct cgaccctggc ttcggcttcg gcaagagcgt cggccacaac tatgcgctgc
     3841 tgcgcgagct cgcgcgtgtc ggcgtcggcg ggttgccggt gctggcgggg ctgtcgcgca
     3901 agtccatgct cggcgcagtc accggtcgcg cggtcggcga gcggcttgcg gccagcgtcg
     3961 ccggggcgct gatcgcggtg cagaacggcg cgcgtatcgt gcgggtccat gacgtcgccg
     4021 ccactcgcga cgcgctcgcg gtgtggaagg cgaccgtgac gggatgctga gcaaggcacc
     4081 gcccgcggtg tcataatccg cgctttcgaa agctgctgga gcgaagatgg cgcgcaagta
     4141 tttcggcacc gatggtgtgc gtggcaaggt cggcgatacg ccgatcacgc ccgagttcgt
     4201 gatgaggctg ggctatgcgg ccggcgtgac cttggtcgcg cgcgagaagc tgcccgcggg
     4261 cgaacacccc gcggtgctga tcggcaagga cacgcgcatc tccggctaca tgctcgaagc
     4321 cgcgctcgag gccgggttcg ccgcggccgg tgtggacgtg atgctggccg gaccgatccc
     4381 caccccggcg gtagcctatc tcacgcgcgc actgcgcctg caggccggcg tggtgatctc
     4441 ggcctcgcac aaccccttct acgacaacgg gatcaagttc ttctccgccg acggcagcaa
     4501 gctgcccgat gcggtcgagg ccgagatcga ggcccacctc gacgagccga tgggttgtgt
     4561 ggactccgcc cgcctgggca aggcgcgccg catcgacgac gccgccgggc gctacatcga
     4621 gttctgcaag agcacctttc ccaacgagct cgacctgcgc ggcctgcgca tcgcggtgga
     4681 ttgcgcgcac ggcgcggcct accagatcgc ccccaaggtc ttccacgagc tcggcgcgga
     4741 gatcatcccg gtgggcgtcg agcccaacgg cctcaacatc aacgacggcg tcggtgcgac
     4801 gcggccggag aacctgcggc aggcggtgct caagcatggc gcggacctgg gcatcgcgct
     4861 cgacggcgac gccgaccgcc tgatcatggt ggaccatcgg ggtgagatct acgatggcga
     4921 caagctgctc tacgtggtgg cctgtgcgcg ccacgccgaa ggccggctgg agggtgtggt
     4981 cggcacgctg atgagcaacc tcggcttcga gcacgcgatc cagcgcctcg gcacgccctt
     5041 cgcacgcgcc aaggtgggcg accgctacgt gctcgagctg ctgcaggagc gcggctggaa
     5101 gattggcggc gagaactccg gtcacatcat ctgcctcgac cgccacacga ccggcgacgg
     5161 catcgtgtcg gcgctgcaag tgctcgccgc gctcaagcag cgcggacttt cgctggcgga
     5221 cgcctgcgcc gatctggtgt tctaccccca gcgcctgatc aacgtgcgca tgcctgccgg
     5281 cttcgactgg cgtgcggacg gctcgatcgc gagcgcacag gccagagccg aacagacgct
     5341 cggcgatagc gggcgcgtgc tgctgcgccc ttcgggcacc gagcccctgc tgcgcgtgat
     5401 ggtggagggc cgcgacggcg agctggtcga gcgtctcgcg cgcgagatcg ccggcgtcgt
     5461 cgaggaagcg atcgcctgat tcgggctgca tgagatggga ggacggggcg caaggctccg
     5521 tccggacgcg ggaagaaggc agatctgccc gttcgatgac acgcagcgtg gctgcgacaa
     5581 tcgctggcta cctgacgggc tcggtcttca accccgaggg gcatcccggc agaggagccg
     5641 acttcggcgc tcgatccgat ctcgacggcg cgtatcgggg atgctgatcg acgaactgga
     5701 ggaagagttc atcatcgtgg tcgtcaccca cagaatgcag caggcggtgc ggagctcgga
     5761 ctacaccgcc tgcatgtacc tgggcgagat ggtggagctc gacgtcaccg acgagcgctt
     5821 cgtgaagccg aacaagaagg agaccgagga cgacatcacc ggtcgctgcg gccgagcaac
     5881 gatcccgggc ggaagagtgc gcgccccgcg ctgcacgagg gctggtcgca ttgcgggtga
     5941 tcgtccgcaa gccgtataat cctgcggttt atctgcacgg atattccctc tcccatgacc
     6001 agactcgttg ccggtaactg gaagatgaac ggcagcctcg ccgccaatcg cgagctgctc
     6061 ggcggcctcg tgtcgatccc gggcgtggaa atcgccgtgt gtgtgccgta tccctatctc
     6121 gcccaggcgc aacaacggcc cgagggcgtc gccctcggtg cgcaggacgt gtcggagtac
     6181 gcggtcggcg cctataccgg cgaggtgagc gcggccatgc tcgcggagtt cggctgccgc
     6241 tacgccatcg tcggtcactc cgagcgccgt gccctcttcg gcgaggaaga tgccacggtc
     6301 gggcgcaagg cgctcgccgc gctgcaggct ggcttggtgc cgatcgtgtg cgtgggcgag
     6361 acgctcgccg agcgcgacga ggcgcgtgcg gtcgaggtga tcggccgcca gctcgaggcg
     6421 gtgcgcgcgg tgctcggcgc cgcggcgatg ggtgcggtgg tgctagccta cgaacccgtg
     6481 tgggcgatcg gcaccgggcg ctccgcgagc gtggcgcagg tggccgaggt gcatgctgcg
     6541 atccgcgcgt ggctgaaggt acacggcgtg gcggcgggtg gcgtccgcat cctctacggc
     6601 ggcagcgtca aggcggacaa tgccgcggcg ctcttcgccg tggaggatgt tgacggtggg
     6661 ctgatcggcg gcgcctcgct cgtcgctgcg gatttcttgg ccatctgccg cgccgcggcg
     6721 ggtggggtct gagtttcaac gattcgtatc tttcaggatt caagatgggt gaatatcttt
     6781 tctctctcgt gcttaccgtg catgtgctgg tgggcctcgg cgtgatcggg ctcgtgctcg
     6841 tgcagcacgg caagggtgca gacatgggtg cagccttcgg cagcggcgca tcgggcagtc
     6901 tcttcggtgc atcgggttcg gcgaacttcc tcagtcgcac cactgcggta ctcgccacgg
     6961 ttttcttcct gaccagcctc gggctcagct tccttgccag caacaagccg gcggcaccgg
     7021 gcagcgtgat ggaaggggcg cagactgcgc ccgcaacgaa cccggctccg gccgcgcccg
     7081 tcgagtcctc gccggcccag tcgatcccga aataattggt tgcgagccct ttgcgcagtg
     7141 cgggaaaaca cttataatta tcgcgctgtc agaacgaagc gccgacgtgg tgaaattggt
     7201 agacacgcta tcttgagggg gtagtggcga aagccgtatg agttcgagtc tcatcgtcgg
     7261 caccacattc cgggaacgcc cgcagaagcg ggcgtttcca cgcagagctg agcaataaca
     7321 ataattgcgg cgcgccaatg acgccgcgga cttcaaatgg gggtttccgg acatgctgga
     7381 aaattatttt cccgtcctga tgttcatgct cgtcggcctc ggcttcggcc ttgcgccggt
     7441 catcctcggg cgactcctcg ccccctatcg tcccgattcc gaaaagaatt ctccctacga
     7501 gtgcggcttc gaggcgtttg aagacgcccg catgaagttc gatgtccgct actacctgat
     7561 cgccattctc ttcatcctgt ttgacctcga aatcgccttc ctctttccgt gggcgacggt
     7621 cttccaggag ttcatcgccg ccggcgaaat cgcttggttc gtattcggct cagtgatggt
     7681 ttttctggcg attctggtca ttggctatat cgtcgagtgg aaaaacggcg cactcgactg
     7741 ggagtaatgc atgagcattg agggcgtctt ccgcgagggg ttcgtcacca cctcgctcga
     7801 tgcggtcatc aactggacgc gcaccggctc gttgtggccg atgaccttcg gtctggcctg
     7861 ttgcgcggtc gagatgatcc acgcgggctg ttcccgctac gacttggatc gcttcggtgt
     7921 tgtgttccgg ccgagtccgc gccagtccga cctgatgatc gtcgccggaa ccttgtgcaa
     7981 caagatggcg ccggcactgc gcaaggtcta tgaccagatg tcggagccgc gctgggtcat
     8041 ctccatgggc tcctgtgcca acggcggtgg ctactaccac tattcgtact cggtcgtgcg
     8101 cggttgtgac cgcatcgtgc cggtcgacgt ctatgtgccg ggctgtccgc ccaccgccga
     8161 ggcgctgatc tacggcattc tccagctcca gaacaagatc aagcgcacga acacgattgc
     8221 gcgctgacgg gtgtcccaga catgagtgcc aagctagaac gcctcagcca gaccttgcgc
     8281 gacgttttcg gtaaccggat ccagtcgctc gtactcgatc gcggtgaagt gacgatcgag
     8341 gtctccgctg ccgaatatcc ccgcgtcgca cgccagcttc gcgaccatgc cgatttgcgc
     8401 ttcgagcagt tgattgacct ttcgggtctc gactactccg cctacggtaa cgcgatctgg
     8461 gagggcaagc gcttcgcttc cgtcgcccac ctgacctcgg tcaagcacaa ctggcgcctg
     8521 cgtgttcgcg tgttcgccga cgacgacgcc ttcccggtga tcgattcggt ggtcgatgtg
     8581 tggccgagcg ctaactggta cgagcgcgag tccttcgatc tgtacgggat cctgtattcg
     8641 ggccacccgg atctgcgccg catcctgacc gactacggtt tcgtcggtca tccgttccgc
     8701 aaggacttcc cggtttcggg gtacgtcgaa atgcgctacg acccggagca gggcagggtc
     8761 gtctatcagc cggtgagcat cgagccgcgc gagaacacgc cgcgcatcgt gcgcgaagag
     8821 aaatacgggg acgtcggcca tggctgagat ccgcaactac acgatcaact tcggtccgca
     8881 gcacccctcg gcgcacggcg tgctccgcct cgtgctcgaa ctcgacggcg aagtcgtcga
     8941 gcgcgccgac ccgcacatcg gcctcctgca ccgcggcacc gagaagctcg ccgagacgcg
     9001 cacctgggtg cagtccgtgc cgtacatgga ccgtctcgac tacgtgtcca tgatgtgcaa
     9061 cgagcacgcc tactgcatgg cgatcgagaa gctgctcggg gtcgaggttc cgctgcgggc
     9121 ccagtacatt cgggtgatgt tcgacgagat tacccgcgtc ctgaaccacc tgctcaacat
     9181 cggtacccat gccctcgaca tcggcgcgat gaccatggtg ctgtatacgt tccgcgagcg
     9241 cgaagacctg atggacgtct atgaggcagt ctcgggtgcg cgcatgcatg cggcgtacta
     9301 ccgtccgggc ggcgtctatc gcgatctgcc ggaccgcatg cccaaatacg aggtcaacaa
     9361 gttcaagaac gagaagacgg ttcgcgagct caacgccaac cgcgaaggct cgatgctcga
     9421 cttcctcgag gacttcacca accgcttccc gacctactgc gacgaatacg agacactgct
     9481 gaccgacaac cgcatctgga agcagcgtac cgtcggcatc ggcgtggtat cgcctgagca
     9541 ggcgctggcc tggggtttca ccggtccgat gctgcgcggc tcgggcatcg cctgggatct
     9601 gcgcaagaag cagccctacg aggtctatga tcgcgtcgat ttcgatgtgc cgatcggcaa
     9661 gaacggcgac tgctacgacc gttacctgtg ccgcatggaa gagatgcgcc agtcgaaccg
     9721 catcatcaag cagtgcatcg actggctgcg caagaacccc ggtccggtca tcaccgacaa
     9781 ccacaaggtc gcgccgcctt ctcgcgaggc gatgaagacc aacatggaag agctgatcca
     9841 ccatttcaag ctctttaccg aaggcatgca cgtgccaaag ggcgaggtgt acgcggcggt
     9901 cgagcatccg aagggcgagt tcggcgtgta tgcggtgtcg gacggggcga acaagcccta
     9961 ccggctcaag ctgcgcgcgc cgggcttcgc gcacctcgcg gccatggacg acatcgcccg
    10021 tggccacatg attgccgacg tggtcgcgat catcggcacg atggacgtgg tgttcggcga
    10081 gatcgaccgc tgagcgcgcc cgcgcgcaac agcggatggg cgcctgcggg cgccgcggta
    10141 tcaaacaaga atcggcaggc tatccggaag atcaagacga catgctgagc caggaatcgc
    10201 tgcaacagat cgatcgagag atcgccaagt atccgcccga tcagacgcag tctgcggtca
    10261 tggccgcgct gcggatcgcg caggtcgaga agggctggct gcccaaggaa ctcatcgagt
    10321 tcgtggccag gtacctcggc atgccgccga tcgccgcctt cgaggtcgcg agcttctaca
    10381 acatgtacga cctcgcgccg gtcggacggc acaagatcac cgtgtgcacg aacctgccgt
    10441 gcgcgctgtc cggcggcgtc catgcggcgg actacatcaa ggaaaagctc ggcatcgact
    10501 tcaacgagac cacgcccgac ggcaagttca cgctgaagga aggcgagtgc atgggcgcct
    10561 gtggcgacgc cccggtgctg ctggtgaata accatcacat gtgcagctgg atgaccaccg
    10621 agaagatcga ccagatgctg gccgagctgg acaagaaatg agcacgcaag gaatgatcct
    10681 cgccggctgc gacggcgacc gcacctggcg gctccaggac tacgtcgccc gcggcggtta
    10741 ttcggcgctc cgcaagatcc tcgccgagaa gataccgcag gacgcgatca tcgccgagct
    10801 caagacctcg gtgctgcgcg gtcgtggcgg agcgggcttc ccgaccggac tgaagtggag
    10861 tttcatgccg cgctccttcc cgggcgacaa gtacctcgcg tgcaactccg acgagggcga
    10921 gccggggacc ttcaaggacc gcgacatcct gcgctacaac ccgcacagcg tcatcgaggg
    10981 catggcgatc gccgcctacg cgatgggtgc tgcgcggggt tacaactaca tccacggcga
    11041 gatcttcgag acctactcgc gcttcgagga ggcgcttgcg gaggcccgcg aggccggttt
    11101 catcggccgc aacatcctcg gcagcgactt ctccttcgaa ctcttcgcgc atcacggcta
    11161 cggcgcctac atctgtggcg aggagaccgc gctgctcgag tcgatcgagg gcaagaaggg
    11221 ccagccgcgc ttcaagccgc ctttcccggc gagctacggc ctctatggca agccgaccac
    11281 gatcaacaac accgagacct tcgcgtccgt cccgttcatc atgaacatgg gcggcgaggc
    11341 cttcctgaac ctcggcaagc ccaacaacgg cggcatgaag ctcttctcgg tctcgggcca
    11401 cgtcaatcgc ccgggaaact acgagatccc gctcggcacg cccttcgccg agctgctcga
    11461 gatggctggc ggcatgcgcg gcgggcgcaa gatcaaggcg gtgatccctg gtggttcgtc
    11521 cgctccggtc gttcccggcg gcgtgatgat ggactgcacg atggactacg actcgatctc
    11581 caaggcggga tcgatgctcg gttccggcgc ggttatcgtg atggacgaga ccacctgcat
    11641 ggtcaaggcc ctcgagcgac tgtcctactt ctacttcgag gagtcctgcg gccagtgcac
    11701 gccctgccgc gagggcaccg gctggctgta ccgtgtggtg catcgcatcg agcacggtct
    11761 tggccgcccg gacgacctcg acctgctgaa ctcggtgacg accaacatca tgggccgcac
    11821 gatctgcgcg ctcggtgacg ccgcctcgat gccggtgcag agcttcgtca agcacttcgg
    11881 cagcgagttc gaataccaca tcgaaaacaa gaagtgcctc gtgccgcccg aagtgcagcg
    11941 cgctggcagt caaatctacg tgagcccgtc atgctagaga tcgaaatcga cggcaagcag
    12001 gtccaggtca acgacggcag cacggtgatc gaggctgcag cggttgccgg gtcctacatt
    12061 ccgcacttct gttaccacaa gaagctgtcc atcgcggcta gctgtcggat gtgcctggtg
    12121 caggtcgaga aggcgcccaa gccgctgccc gcctgcgcga cgcccgttac caatggcatg
    12181 aaggtatgga cgcactccga gctcgccgtg aaggcgcaga agggggtgat ggagttcctg
    12241 ctgatcaacc acccgctcga ctgtccgatc tgtgaccaag gcggcgagtg ccagctgcag
    12301 gacctcgcag tgggctacgg ttcgaccgcc tcgcgctacc aggaagagaa gcgcgtggtg
    12361 ttcaacaaga accttggcgc gctggtgtcg accgacatga cgcgctgcat caactgcacg
    12421 cgctgtgttc gcttcaccac cgagatcgcc ggcgagatgg agctcggcca ggccttccgc
    12481 ggcgagcatg cggagatcat gcccttcatc gagaagacgg tcgattccga gctctccggc
    12541 aacatcatcg acctctgccc ggtcggtgcg ctgacctcga agcctttccg cttcgccgct
    12601 cgtacctggg agctctcgcg ccgcaagtcg gtgagcccgc acgactccct cggcgccaac
    12661 ctgatcgtcc agaccaagca cgacgtggtg aagcgcgtgc tgccgttcga gaacgaagac
    12721 gtcaacgagt gctggctgtc cgacaaggac cgcttctcct acgaggccct gtccagcgag
    12781 cagcgcctcg gtgcgccgat gatccgccaa ggcgggcagt ggaagcaggt cgagtggcag
    12841 gccgccctag agttcgtcgc caccggcctg cgcggcgtcg agcgcgatca tggtgccgcc
    12901 tcgatcggcg cgctggcctc gccgcatgcc acggtcgaag agctccacct gctgcagaaa
    12961 ctgatgcgcg ggctcggtag cgagaacatc gatttccgcc tgcgccaggt cgacttccgt
    13021 gccgacggca agcgcgccgg cgctccgtgg ctggggatgc cgatcgccgg cgtcaaggat
    13081 ctgacccgcc tgctcgtggt cggcagcttc ctgcgcaagg atgcgccgct gctggcgcag
    13141 cgcgtgcgcc aggccgcaaa gaaaggcctg cacgtcagcg ccatcggccc gaacgccgaa
    13201 gagtggttgc tcccggtgcg caaccgcgcg ctggtcgcgc cttcggcgat ggtcgccacg
    13261 ctcggccagg tcgtggtcgc gctggcgaag gagaagggcg ccgaggtcag cgtcgcgctg
    13321 ctcggcaaac tgcccgagac agtgagcgat gaagcgctcg ccatcgcgca gagtctcgcc
    13381 agcggccgca aggttgcggt gtggctcggc aatctcgccg tccagcacgc ccgcgcttcc
    13441 gagctgcaga tgctcgcgca ggagatcgcg cgcctcaccg acggtagctt cggcttcatc
    13501 ggcgaggctg ccaacagcgt cggcggctat cttgccggcg cggttcccgg cgcgggcggc
    13561 ctgaacgccg ccgcgatgat cgagcagcca cgtgcggcct acgtgaccct gaacctcgag
    13621 cccgacctcg accttgccaa tccggcggcg gcgatggcgg cgctcgacgg tgcccagctc
    13681 gtggtggcgc tcacgagctt cgactcgccg tcgctgcggc aggtggccga cgtgatgttg
    13741 ccgatcgccc ccttcaccga gacctcgggc accttcgtga actgcgaggg ccgcgcgcag
    13801 agcttcaccg cggtggccaa gcccttcgcc gatgtccgtc cgggctggaa ggtgcttcgc
    13861 gtgctcggta acctgctcgc gctcgagggt ttcgcccacg ccgactccga ggccgtgcgt
    13921 gccgaggcgc tcgcgggtga tctcgccagc cggttggaca atggcgtgga aggtatcgtg
    13981 gtctcttcgg ctaccggtcc ggcaggcgtg ctcgagcgtg tagccgacgt gccgatccac
    14041 ttcgccgatc cgatcgcgcg ccgcgccccg tcgctgcaga agacgcgcga cgcagcggca
    14101 cccacggcgc gaatcgcgcc ggcaacgctc gcggcgctcg gcctcgcggc cggggaccgt
    14161 gtgcgcgtgg cacagggtac ggcgagcgtg gaactcgtgg cccaggcgga tgatggattg
    14221 gctgcaggat gcgtgcgcgt cgccgccgcg catccctcga ccgcggcgct cggcgcgatg
    14281 agcggtgatc tcagcgtgga gcgtgcgtga tggaagcgat gctgcaaccc gtggcggatt
    14341 tcttcggccc ctcctggacg gccatctgga cgctgctcaa gatcgtcgcg atcatcgcgc
    14401 cgctgatgat ttgcgtcgcc tacctgacgc tcgccgagcg caaggtcatc ggctacatgc
    14461 aggtccgcat cggccctaac cgggtcggtc cgaagggcct tctgcagccg atcgccgacg
    14521 gcatgaagtt gctgttcaag gagatcatcg tcccgagcgg ggcgagcaag ggcctcttca
    14581 tcctcggtcc catcctggcg atcgcgccct cgctggcagc ctgggcagtg gtgcccttcg
    14641 acgacggcct ggtgctcgcg gacgtcaatg ccggtctgct cttcctgctc gccatcactt
    14701 cgatggaggt gtacggggtg atcgtcgcag gttgggcgtc gaactcgaag tatcccttcc
    14761 tcggttcgat gcgtgccgcg gcgcagatgg tgtcctacga ggtgtcgatg ggtttcgcgc
    14821 tgatctgcgt gctgctcatc tcggccagcc tcaacctcac cgacattgtc atgtcgcaga
    14881 acgaaggccg cttcgctgac atgggcctgg gcgtcctgtc gtggaactgg atcccgctct
    14941 ttcccatgtt cgtggtgtac gtgatctcgg gcatcgccga gaccaaccgt gcgcccttcg
    15001 acgtggtcga aggcgaggcc gaggtggtcg cggggcacat ggtcgagtac tcgggcatga
    15061 ccttcgcgct gttcttcctc gccgaatacg cgaacatgat tctggtgtcg atcctgacct
    15121 cgatcctgtt cctcggtggc tggctgtcgc ccgtgggctt cctgcccgac ggtttccact
    15181 ggctggcggt caagaccgcg ttcatcctgc tgctgttcct gtgggcgcga gccaccttcc
    15241 cgcgcttccg ctacgaccac atcatgcgtc tgggctggaa ggtgttcatc ccggtcaccc
    15301 tcgtgtggct gttcgtggtg accgtgtgga tgatgtcgcc gctgtcgatc tggagttgag
    15361 aagaccatgg gtgccaagga ttacatcggc agtctgttcc tgaaggaact tgtcaagggc
    15421 atggccctga ccggacggca tttctttcag cgcaagatca ccatcctctt cccggaagag
    15481 aagacgccgc agagcaaccg tttccgtggc ctgcacgcgc tgcgccgcta cccgaacggc
    15541 gaggagcgct gcatcgcgtg caagctgtgc gaggcgatct gcccggcaat ggcgatcacg
    15601 atcgagtccg atcagcgtga cgacggatcg cgccgcacga cgcgctacga catcgacctg
    15661 accaaatgca tcttctgcgg cttctgcgag gaagcctgtc cggtggatgc gatcgtcgag
    15721 actcgcgtgc tggagtacca cggagagcag cgcggcgatc tgtactacac gaagcagatg
    15781 ctgctcgcgg tgggggatcg acacgagtcg cagattgcgg cagaccgcca acaggacgcc
    15841 aagtaccgct aaggggaggg cgcctcggcg ccacgagtca agaacatgga attcaagacc
    15901 ttcgtcttct actttctcgc cgcgatcatg gtgctcgcgg cgctgagggt gatcaccgcg
    15961 cgcaacccgg tacacgccgc gctcttcctc gtgctcacct tcttcaacgc tggcggtctc
    16021 tggctgttgc tgcaggccga gttcctcgcg gtcacgctcg tgatggtata cgtcggcgcg
    16081 gtgatggtgc tcttcctgtt cgtggtcatg atgctcgaca tcaacctcga ccgtctgcgt
    16141 gaaggcttct ggagctatct cccggtggga gccctggtgg gcatcctgat gctggtcgag
    16201 atggtgatgg tgctcggcgg cggctacttc ggtctcgagg cgatgcctgc gcctgctccg
    16261 gttgccgcgg actacagcaa cacccgcgaa ctcggccgcg ttctctacac cgattatgtg
    16321 tatgccttcg aactcgcctc gctcgtgctg ttggtggcga tggtctccgc agtggcgctg
    16381 acgttgcgca agcgcaaggg gctgaagtcc atcccgccgt ccgagcaggt cgccgtcagg
    16441 cgcgagggcc gggtcgagct ggtgaagatg caggccgaga aagacgactg acaacgtacg
    16501 cgaacaaaac aagaaccgcg ccggcaggca gccggcgcga tcagggggtc actgatgctt
    16561 tcgctttccc atttcctcat cctgggcgcg atcctgttcg cgatcagcgt ggtcgggatc
    16621 ttcctcaacc ggaagaacct gatcgtgctg ctgatggcca tcgagctgat gctgctctcg
    16681 gtcaacatga acttcgttgc gttctcgcat tacctcggcg acatcgccgg ccaggtcttc
    16741 gttttcttca tcctcaccgt ggcggcggcc gaatcggcga tcggcctcgc gatcctgatc
    16801 gtgatgttcc gcaacatgcg cacgatccac gtggatgatc tggacagcct caagggttaa
    16861 ggaagcgtca cgatgtcgga catgaaagca atctatctcc tcgtgccgct ggcgccgctg
    16921 gcgggagcga tcctcgcagg cctgttcgga aagcagatcg ggcgtgccgg ggcgcatgtg
    16981 gtcacgatcc ttggtgtggc gatcgccttc gtggcctcgc tcttcatcta ccaggatgtg
    17041 gcggccggaa acaccttcaa cggcaccgta tatacctgga tgcaggcggg tggcatcgac
    17101 ttcgaggtcg gtttcctgat cgactcgctg acggtgatga tgatgctggt cgttaccttc
    17161 gtgtcgctga tggtgcacat ctacaccatc ggctacatgt cggaagatcc gggctaccag
    17221 cgcttcttca gctacatctc gttgttcacc ttctcgatgc tgatgctggt gatgtccaac
    17281 aacttcctcc agctcttctt cggctgggag gcggttggcc tggtgtccta cctgctgatc
    17341 ggcttctggt acgagcgtcc gacggcaatc tacgccaaca tgaaggcctt cctggtcaac
    17401 cgcgtgggcg acttcggctt cctgctcggc atcggcctcg tcgccgccta tgccggcagc
    17461 ctcgactacg ctgaggtttt cgccaaggcg aaggaactct ccgtgaccga gatggcggtg
    17521 accggctggc cgctgctcac cgcgatctgc atctgcctgt tcatcggtgc gatgggcaag
    17581 tccgcccagg tgcctctgca cgtgtggctg cccgactcga tggaaggccc gaccccgatc
    17641 tccgcgctga tccacgcggc gaccatggtg accgcgggca tcttcatggt ggcgcgcatg
    17701 tcgccgctgt tcgagctgtc
//
LOCUS       DAOQNN010000022        17682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1200_length_17682_cov_9.669484, whole genome shotgun sequence.
ACCESSION   DAOQNN010000022 DAOQNN010000000
VERSION     DAOQNN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 17682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17682
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1200_length_17682_cov_9.669484"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..821
                     /locus_tag="PKV97_02805"
     CDS             228..821
                     /locus_tag="PKV97_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB04834.1"
                     /translation="MENIVKKPRTQLDRAAWIAGATEVLAEEGIAGLRVEVLAKRLKV
                     TKGSFYWHFTDRRDLLMAVLAQWKEGRIRDIIKQTRAQPGRELEQIYHVIDVYSASRS
                     RRGMMIELAVRDWARRDAEAAAIVAEVDDVRLRCARDLFLACGVPMDEASSRCMLLYA
                     YVFGVSLMIYEKFDRDVVRLKRDIADLIARSPSRVVA"
     gene            complement(880..1752)
                     /locus_tag="PKV97_02810"
     CDS             complement(880..1752)
                     /locus_tag="PKV97_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB04835.1"
                     /translation="MKNGMALLAAIGIATLSASAQAALIRLEVSGNLAYGTDRTNVFG
                     LGANTSLTGRAATFSWLMDSASLGSDRNADPTLSAYGCGSAAPGCTYFMSASVTINGV
                     TRTLTGRGSDLTNDLRMYADTNGPALGSADGYYLRMGQTRDVGNWYLDEGRNWYRAAT
                     RITSKVEIGVNDAVDSILAGLDPAELHGWSTSLLTGTNDDGSTVFVFSETDLNGVQIF
                     EYATGGLLLTDRSTRWWTDTAPSSGSGTGGTNNPGGGTNAPAITVPEPATIALLGLGL
                     GLLRRKSRRHSGRA"
     gene            complement(1984..2760)
                     /locus_tag="PKV97_02815"
     CDS             complement(1984..2760)
                     /locus_tag="PKV97_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNB04836.1"
                     /translation="MLIDTHVHLDAAEFDDDREQVIARARAAGVGRFVVPAVERRGFD
                     AVERLADDHRGIVHALGIHPLYTMRAAREDLDLLDQRLNRGRAIAVGEIGLDHYVTDV
                     DRGVQLEFFTAQLRLAARHGLPVILHVRRAIDPILAQLRRIPVPGGIAHAFNGSRQQA
                     DILIGMGFKLGFGGSMSYGGSTRIRELAASLPLEAIVLETDAPDIAPEWARGRRNEPA
                     NLARYAGILAELRGMAPEEVADVTTANAIQVLHWPPLEEE"
     gene            complement(2804..3613)
                     /locus_tag="PKV97_02820"
     CDS             complement(2804..3613)
                     /locus_tag="PKV97_02820"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNB04837.1"
                     /translation="MNAEFRSDAAAEAPILRREDAGGVTTLTLNRPQQFNALSFEMLE
                     ALIAAVDEIARDETVRVVVIAGEGKAFCAGHDLKEMRGNHTLEFQQRLFRLCGKFMMK
                     LTELPQPVIARVHSIATAAGCQLVSMCDLAVAAEHARFAVSGINVGLFCATPGVGLSR
                     NMGRKEAFEMLVTGDFIDAREAQRRGLINRVVPADQLDEEVGRLAASILAKSPVAIRM
                     GKQMFYKQLEMGLDAAYQLASETMACNAMCEDAAEGIDAFIAKRKPEFKGR"
     gene            3837..5528
                     /locus_tag="PKV97_02825"
     CDS             3837..5528
                     /locus_tag="PKV97_02825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB04838.1"
                     /translation="MNLRTRFLLLISALIVVGSVSAWAVFHHLARQIVMQWGQRVADI
                     QVRYDRARLLQPLEREIALARQMADSLVLKRWARSPVDPALEAEAVREMENFRRNFSS
                     GNYFVALRTSGAYYHNNAGDEFAGRQLRYHLDPAKPADAWFYKLVQVGPDFHLNVNPD
                     AELGVTKLWIDVLIRDGDEILGVVGTGIELTRFLDEIVDLHQDGVSTLFADLSGAIQL
                     HRDAKLIDFASLVKPEGQKSLADQLLDRESDRVALRETMSALAASGDGVPRVLDRFVE
                     SGGRRTLLGIAFVPEIGWYEITLLDLDVLMPVAALTPVAVAFVLTLLVALVLVHLVLR
                     RLILDPLGRLEQAMLRVRDGDLSPAPLAQASGEIGRLTQHFEAMAGAIRAHTAELEAK
                     VRERTDALHRLARIDPLTNLTNRRGMSEMLEAEIIRAERQGSRFGLLWLDVDHFKEIN
                     DRRGHGAGDEALTRLARVLEGCLRPYDRAARWGGDEFLVLLSPCDAATLEAIAHRVRA
                     DIEHGSAACGERVTVTIGACLAEPGETLGVVLQHADDALYRAKDAGRNQVVIAAR"
     gene            complement(5601..6239)
                     /locus_tag="PKV97_02830"
     CDS             complement(5601..6239)
                     /locus_tag="PKV97_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNB04839.1"
                     /translation="MQPRHPDCHRVLPRLSACIATALLVAACQVLPERPRAAATYDLG
                     TGERAALAHGLAPARVAVSSPPWLQTTTMHYRDARVDPGRRRAYADNRWAAPPPAMLG
                     LVLERTLGAGEGGGCRLQVQLDEFEQVFTAGERSEARLLARFALLPVRGAAVIASASL
                     VVAEPATTADASGGASAFRAATERLALAIAQWIAETDRALSGGLGIAGRCQR"
     gene            complement(6257..7174)
                     /locus_tag="PKV97_02835"
     CDS             complement(6257..7174)
                     /locus_tag="PKV97_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNB04840.1"
                     /translation="MENRAHALAAGLFAILLGAGVVFAIWWFSGQRVPMREIVLEARG
                     DINGLGAQSRVRYRGMAVGSVRAVAIDPEDLRTLLVRIAVPADLPLTRGTTAALGTLG
                     VTGLAFVQLDDRGVDRRPLAAADGEVPRIALQPGLVEALSGRALAALDQFQALGERLQ
                     GALASLESAAAGVDRGVAELPATLAALRAALSAGNIARITSVLGNLERGSAEAVPAVA
                     ELRRLIVRIDQAAARLELRAAAAGDDLVERTLPQLDVLLGELTSTSQRFGHLVEELDA
                     SPQLLLTGRDRPLPGPGEAGHEGFAGSDR"
     gene            complement(7176..7940)
                     /locus_tag="PKV97_02840"
     CDS             complement(7176..7940)
                     /locus_tag="PKV97_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB04841.1"
                     /translation="MVCLEGIVTRFGRNIVHEGLDLTIRAGDIVALVGGSGSGKTTLL
                     RHLVGLSRPAAGRVEVFGEALDAGGAAARLARQRRMGVLFQHGALFSAFSVAQNIAFP
                     LRELGVISRPEIDRLVALKLAMVEMEPAHAPLMPAELSGGMVKRVALARALALEPELL
                     LLDEPTAGLDPDRSASFIRLIRALHRELGLTVVLVTHDLDTLAQMATSVAVLAERRIV
                     SHASLADTLAYDHPFVARFFSGLQARCGQPRGAGAG"
     gene            complement(7964..9079)
                     /locus_tag="PKV97_02845"
     CDS             complement(7964..9079)
                     /locus_tag="PKV97_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB04842.1"
                     /translation="MAGRVLDEVFTAGAEGIRLAGDWTLRGLDECLPTLRRALATVPP
                     DARWSLRAVRRMDSFGALLLWQAWGGRWPAALEADDAQRAIIGRIENASARALPEPPR
                     ASALDAVALLGKGLFAFAGHLSGFVALLGRLVLDLGHLLHHPRDWPLLEISANLHKVG
                     VKAMPVSALVGFLIGVVLSYLSALQLRTFGADAFIVNILGMGIIRELGPVLVSVLVAG
                     RSGSAMTAQLGVMRVTEEIDALAAMGVSRSLRLVLPKVVALSLAMPLLVLWTSAIALF
                     GGWVSAWVELGIGLQFFLRALPDAVPVANLYIGLAKGAVFGFLIALVACHFGLRVKPD
                     TESLSANTTASVVAAITVVILVDAVFAIATRSIGLPL"
     gene            complement(9086..10585)
                     /gene="ppx"
                     /locus_tag="PKV97_02850"
     CDS             complement(9086..10585)
                     /gene="ppx"
                     /locus_tag="PKV97_02850"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNB04843.1"
                     /translation="MQDLIAAIDLGSNSFRLQVGRIVNDQIYPLDGLKEAVRLAAGLS
                     PDKMLDLASQQRGVGALQRFHERLRDFPPTRVRAVATNTLRVAKNAPEFLIRAEAALG
                     FPIEIIAGREEARLIYVGVAHTLPDPHRQQLVVDIGGGSTEFIIGKSFESQLLESRYM
                     GCVGYSLRYFPEGRIDKRAFKEAELAARRELQTISAAYREAGWEEAVGSSGTAKAIVE
                     ILEQNGLSASGITREGLEKLKALLLRAGSLDNIDLAGLKGDRLPVILGGFAIMSAIFK
                     EFELEHMVFSEGALRLGVLYDLLGRYHHYDLRDATVSAFVRRYGVDLRQAQQVADTAC
                     HLLAQLEPETADPEHDDHRFLRWAAMLHEIGISVAHSSYHKHSAYIVGNADMPGFSRM
                     DQARLARLVLAHRGKLERVASIDPGSVDWLLIACMRLAVVIHRARDARGLPPVTLRRE
                     GRGFSLTAAPGWLQKLPLTAAALDEEQRQWQGLGRALYIRALSLRTPVA"
     gene            10768..12849
                     /gene="ppk1"
                     /locus_tag="PKV97_02855"
     CDS             10768..12849
                     /gene="ppk1"
                     /locus_tag="PKV97_02855"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNB04844.1"
                     /translation="MPVDRMTLNYPPEHFLNRELSLLQFQRRVLAQAADPTVPLLERL
                     RFLCIVSSNLDEFFEIRVSGIKEQIRIGSRKSGEDGIAPVELLERVSDEVHEIISEQY
                     QLLNGDILPALEKEGVVFLRRSLWTEAQRAWIHDYFMREVMPVLTPIGLDPAHPFPRV
                     LNKSLNFAVELEGRDAFGRDSGAAIVQAPRALPRVIRLPRELCDQEYGFVFLSSVLHA
                     HVGELFSGMNVLGCYQFRVTRNSDLFVDEEEVKDLRASLKGELQQRHFGDAVRLEVAD
                     NCSEEMAGFLLQHFHLTPDDLYRTPGIVNLVRLMQVPDWVERPDLKYAPFVPGLPKVL
                     DKRRQVFTAIRGGDILLHHPFQSFAPVIEMLRAAADDPQVLAIKMTVYRTGTDSVLME
                     HLARAAQKGKEVTVVLELMARFDEEANITLANRLEEVGAHVVYGVFGYKTHAKLLMIV
                     RREEDGLRRYVHMGTGNYHPRTTRFYTDFGLLTCNAEIGEDVATVFKQLTGLGKATEL
                     RHLWQAPFTLQPNVVAAIRREAEIARAGRRGRVIAKMNALLEPETIEALYEASQAGVE
                     IDLIVRGPCALRPGVPGLSDNIRVRSVIGRFLEHHRIFHFHADGAEHMYLSSADWMDR
                     NFFRRIEIAFPVLDPRLKRRVMKEGLRPYLGDNCQAWDMQADGKYRRKHPRSIRRAAQ
                     LILLKELAASS"
     gene            complement(12964..15168)
                     /locus_tag="PKV97_02860"
     CDS             complement(12964..15168)
                     /locus_tag="PKV97_02860"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNB04845.1"
                     /translation="MVSVTHAISQGDTAPPIDLLAAGLEPTEREHIEAALEWIADLYE
                     GKVLGTGEPTWTHAIGAALIAASLRLDAETRVAALLFAAWEELEAPAEEIEARFGPAI
                     TALVRGLHKLNGLRVLTRLTTNASAPEIRAQTEVLRKMLLAMVEDIRVVLVRLASRTQ
                     TLRFYTDLPGEARVEVARESLDIYAPLANRLGVWQIKWELEDLSFRFIEPDTYKRIAK
                     MLDERRVEREQFIRDSIERLKSELAAVGIEAEITGRAKHIYSIYNKMRKKRLDFSQVF
                     DIRALRVLVPEIKDCYTVLGIVHQMWQPIAKEFDDYITKPKGNNYQSLHTAVLAGDGR
                     ALEVQIRTFDMHKHAELGVAAHWRYKEGARQGGDYDEKIALLRSLLSWRDEVTDAAHW
                     VEQLKRASLDDTLYVLTPQGRVVDLPRGATPVDFAYRLHTDVGHHCRGAKVDGHLIPL
                     NTQLESGQTVEIVTAKEGGPSRDWLDTRQGYVATSRARSKIKQYFAQLDEEEILSRGR
                     SFVTKEMQRDGHGQANIDGLAERLGFKNAEALYLAAGRGEVGPRAMQTALREGSAPEP
                     AAEAEPEFVVSRSRSGDNQDKILIVGVGKLMTSLSRCCKPAPPDAIEGFVTRGRGISI
                     HRVDCADFQDLARKHPERVIPAEWGEKAHDTRNALYQVDINVQASDRQGLLRDISEVL
                     SREKLNVIAVNTLTKKGTAYMRFTMEVGGIKQVQRAITLVREVPGVIDAQRK"
     gene            complement(15252..15989)
                     /locus_tag="PKV97_02865"
     CDS             complement(15252..15989)
                     /locus_tag="PKV97_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB04846.1"
                     /translation="MNRPLALDRIRVVLSRTTHPGNIGAAARAMKTMGLRELWLVAPE
                     SFPDEVATARASGAADLLDAARVVGTLEEALADTVFSAALTARRRELSLPRLQARDAA
                     LELVRRTQDGAVALVFGNETSGLTNEEAALCSLPVTIPTDAGFSSLNLGAAVQVLAYE
                     LRMAALGDAAAPADPQPEPATHADFEGFMAHLERAVTASGFHDPANPKRLLPRMRRLF
                     NRVRLEREEVALLRGMLTTFETPRRRG"
     gene            16133..16933
                     /locus_tag="PKV97_02870"
     CDS             16133..16933
                     /locus_tag="PKV97_02870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNB04847.1"
                     /translation="MHPTLNIAVKAARRAASVINRASTQLDLLTVQSKSPNDFVTEVD
                     RAAEQAIIEVLRDAFPGHGILAEESGESGPESEYTWIIDPLDGTTNFIHGMPQYAVSI
                     AQTKNGVLEHAVVYDPNTNEMFTASRGAGAFLNDRRIRVSRRTRLNEALIGTGFPFRQ
                     FDHVDAYLAMFKELTQKTAGIRRPGAASLDLAYVASGRFDGFWEMGLSPWDMAAGVLL
                     IQEAGGLVSDLSGEANYLTTGNLVAGTPKIFGQLLPIIQAWRPANLRA"
     gene            complement(16955..17476)
                     /locus_tag="PKV97_02875"
     CDS             complement(16955..17476)
                     /locus_tag="PKV97_02875"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HNB04848.1"
                     /translation="MNTPDADTRRIGRRLGLLATLAFLALGWVSFDGALERREDPNRN
                     LVVAPGASELVLQRNRAGHYLLPGEINGRPVRLLLDTGATQVSVPARLAAELGLQAGA
                     PASVLTANGAVVVRQTRIPRLAFGPFEFSDVAGHLNPGMQGDEVLLGMSALRHLEFTQ
                     RGDTLTLRAPGSG"
     gene            17469..>17682
                     /locus_tag="PKV97_02880"
     CDS             17469..>17682
                     /locus_tag="PKV97_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04849.1"
                     /translation="MFKSVFPSIHGDNPAFADDSTATGRFLQLAIRKPPFGDTAIRPA
                     ALSEAEIGAHTPMMQQYLRIKQQHPDT"
BASE COUNT         2792 a         6182 c         6055 g         2653 t
ORIGIN      
        1 acgcgaacct tgacgttgta atccaccacg cgctttacgg gtacgagaat cttcactctg
       61 acgcgctcct cttctttgca ggggatggta ggcccgaaac gcggacccga ctttcggatt
      121 atgtcaccgc ccttggccgg tcgcactgct gcgcagcagc atttccatac ggccaagtat
      181 ggcgcatacg gtagcgtatg gtcaccgaat cgtcaatact acgccgtatg gaaaacattg
      241 tgaaaaagcc gcgtacccag ctcgaccgtg ccgcctggat cgcaggcgcc accgaggtgc
      301 tcgccgagga aggcatcgcg ggcttgcgcg tggaggtgct ggcgaagcgg ctcaaggtca
      361 ccaagggcag tttctactgg catttcaccg accgccgcga cctgctgatg gccgtcctcg
      421 cgcagtggaa ggaaggtcgc atccgcgaca tcatcaagca gacgcgggca cagcctggcc
      481 gcgagctcga gcagatctac cacgtcatcg acgtctacag cgcgagccgc agccgtcgcg
      541 gcatgatgat cgagcttgcc gtgcgcgact gggcgcgtcg ggacgccgag gccgcggcga
      601 tcgtcgccga ggtcgacgac gtccgcctgc gctgcgcacg cgacctcttt ctcgcctgcg
      661 gggtgccgat ggacgaggcc tcgagccgct gcatgctgtt gtacgcttac gtgttcgggg
      721 tctcgctgat gatctacgag aaattcgatc gcgacgtcgt ccggctcaag cgcgacatcg
      781 ccgacctgat cgcgcgctcg ccgtcgaggg tggtcgctta gcgcctgcgc ggcagcgcac
      841 gcgccgagcg atgcgcgcgc gcaggcgtcg agggcggaat caggctcgac cggaatggcg
      901 cctggatttc ctgcgcaaca atccgagccc caagccgagc agggcgatcg ttgcgggctc
      961 cggcaccgtt atcgcgggag cgttcgtgcc accgcccggg ttgttggtcc ccccggtgcc
     1021 cgaacccgaa ctgggcgcag tgtcggtcca ccaccgcgtg gagcggtccg tcaacaacag
     1081 cccaccagtt gcgtactcga aaatctgcac gccgttgaga tcggtctcgc tgaagacgaa
     1141 gaccgtgctc ccgtcatcgt tcgtgcccgt cagcaggctg gtggaccagc cgtggagctc
     1201 cgccggatcg aggccggcga ggatggagtc gacggcatcg ttcacgccga tctccacctt
     1261 ggacgtgatc cgcgtcgcag cccgatacca gttgcggccc tcgtccagat accagtttcc
     1321 gacatcccgc gtctgcccca tccgcaggta gtagccgtcc gccgagccga gagccgggcc
     1381 attggtgtcc gcgtacatcc gcaggtcgtt cgtgaggtcg ctcccccgcc ctgtaagggt
     1441 gcgcgtgacc ccgttgatgg tgaccgatgc gctcatgaag taggtgcagc cgggggctgc
     1501 ggatccgcat ccgtaggcgg agagggtcgg atcggcgttg cgatcggatc ccagcgaagc
     1561 agagtccatg agccacgaga aggtcgcggc gcgtccggtg aggctggtgt ttgcccccag
     1621 gccgaagacg ttggtccgat cggtcccgta ggcgagattg cccgaaacct cgaggcggat
     1681 gagcgcagct tgcgcgctcg ctgaaagggt ggcgatgccg atggcggcga gcagggccat
     1741 tccgtttttc atgttgtttt ctcccggttg cgggcgtacg aggcccgtcc aataagcgtt
     1801 aactcgaaac ctcgcggcac aagggtatgc attcatcacc gcgacggttc ggctaaagca
     1861 aatttcgaac caggaagctc ttccccatca atacagacac ttgccgagtc gatcgagtgg
     1921 ctcgcgaaaa aaatgtaaaa aaaatcgaca agcgggcgcc ggggatctcg gcgcccgcgt
     1981 cgcctattct tcttcgaggg gtggccagtg caggacctgg atggcgttgg ccgtggtcac
     2041 gtccgccacc tcttcgggcg ccatccctcg cagctccgcc aggataccgg cgtaacgggc
     2101 gaggttcgcc ggctcattgc gcctgccgcg cgcccattcc ggcgcgatgt cgggtgcatc
     2161 ggtctcgagg acgatcgcct ccaagggcag cgaggcggcg agctcgcgga tccgggtgga
     2221 tccgccgtag ctcatcgatc cgccaaaacc cagcttgaac cccatcccga tcaggatgtc
     2281 cgcctgctgg cggctgccgt tgaacgcatg tgcgattcca cccgggaccg ggatgcggcg
     2341 cagttgcgcg agtatcggat cgatcgcacg ccgcacgtgc aggatcaccg gcagtccgtg
     2401 gcgagcggcc aggcggagtt gtgcggtgaa gaactcgagc tgtacgccgc gatcgacgtc
     2461 ggtcacgtaa tggtcgaggc cgatctctcc gacggcgatc gccctgccac ggttcaggcg
     2521 ctggtcgagg aggtcgaggt cttcccgtgc cgcccgcatc gtgtagagcg ggtggatgcc
     2581 gagcgcatga acgatgccgc gatggtcgtc ggcgagccgc tcgaccgcat cgaacccccg
     2641 gcgctcgact gcaggaacga cgaagcgccc tacccccgcg gcgcgcgcac gcgcgatgac
     2701 ctgctcgcgg tcgtcgtcga actccgcagc atcgaggtgg acgtgggtgt cgatcagcac
     2761 cggtcgcctt cgcattgctc gacggggatg atcccgacgc cgctcagcgc cccttgaact
     2821 cgggtttgcg cttggcgatg aaggcatcga tgccctcggc cgcgtcctcg cacatcgcgt
     2881 tgcaggccat ggtctcggag gcgagctggt aggcggcgtc caggcccatc tcgagctgct
     2941 tgtagaacat ctgcttgccc atgcggatcg ccaccggcga tttggccagg atggacgcgg
     3001 ccaggcggcc gacctcctcg tcgagctggt cggccggcac cacgcggttg atcagcccgc
     3061 gccgctgcgc ctcgcgggcg tcgatgaaat cgcccgtcac cagcatctcg aaggcctcct
     3121 tgcgccccat gttgcgcgac aggccgacgc cgggcgtggc gcaaaacagg ccgacattga
     3181 tgcccgatac cgcgaagcgc gcgtgctcgg ccgccactgc gagatcgcac atcgacacca
     3241 gctggcagcc cgcggcggtg gcgatgctgt gcacccgcgc gatcaccggc tggggcagct
     3301 cggtcagctt catcatgaac ttgccgcaca ggcggaacag gcgctgctgg aattccagcg
     3361 tatggttgcc gcgcatctcc ttgaggtcgt ggccggcgca gaaggccttg ccttcgccgg
     3421 cgatgaccac cacgcgcacc gtctcgtcgc gtgcgatctc gtcgaccgcg gcgatcagcg
     3481 cctcgagcat ctcgaacgat agcgcgttga actgctgcgg acggttcagc gtgagggtgg
     3541 tcacgccgcc ggcatcctcg cggcgcagga tgggggcttc ggctgcggca tcggagcgga
     3601 attcggcgtt catggttctc tcctcgtggt ttcgtgttcg cagggccatg tggccacggg
     3661 cggcaacgtt aacgtcaaca agcgcgccac gccagcgctt cgcgcagcgc tccgcccatc
     3721 acgattcgtc gcgccatgga tccgggtgtg agaaaacgca cacctgcgcc cgcgccggca
     3781 gcccaaactg agcaagaagc cacggacagc gacccgaacg cagggagtgc cggcgcgtga
     3841 acctgagaac tcgatttctc ctcctcatct ccgcgctcat cgtggtcggc tcggtatccg
     3901 catgggcggt gttccatcat cttgcccggc agatcgtcat gcagtgggga caacgcgtcg
     3961 ccgacatcca ggtgcgctat gaccgcgccc gcctcctgca accgctcgaa cgcgagatcg
     4021 cgctggcgcg gcagatggcc gactcgctcg tgctcaaacg ctgggcacgc tcgcccgtcg
     4081 atccggcgct cgaagccgag gcagtgcgcg aaatggagaa cttccgccgc aacttcagct
     4141 ccggcaacta tttcgtcgcg ctgcgcacct ccggggccta ttaccacaac aacgccgggg
     4201 acgagttcgc cggccgccag ctgcgctacc acctcgaccc cgccaaaccc gccgacgcgt
     4261 ggttctacaa gctcgtccag gtcgggcccg acttccacct caacgtcaat cccgatgccg
     4321 aactgggcgt caccaagctg tggatcgacg tcctcatccg cgacggcgac gagatcctcg
     4381 gcgtggtcgg caccggcatc gagctgacac gctttctgga cgagatcgtg gacctgcacc
     4441 aggacggcgt gagcacgctg ttcgctgatc tttccggtgc gatccagctt caccgcgacg
     4501 cgaagctcat cgacttcgcc agcctggtca agccggaagg gcagaagagc ctggcggacc
     4561 agctgctcga tcgcgagtcc gaccgcgtgg cactgcgcga gacgatgagt gccctggcgg
     4621 cgtcgggcga cggcgttccg cgggtcctgg accgctttgt cgagtccggc gggcggcgga
     4681 ccctgctcgg aatcgccttc gtgcccgaga tcggctggta cgagatcacc ctgctcgacc
     4741 tcgacgtact gatgccggtg gcagccctca ccccggtcgc agtcgccttc gtcctcaccc
     4801 tgctggtcgc gctcgtcctc gtccatctgg tcctgcgtcg cctcattctc gaccctctgg
     4861 gcaggctgga gcaggccatg ctgcgggtgc gcgacggcga cctcagcccc gcgccgctgg
     4921 cgcaggcgag tggagagatc gggcgtctca cccagcactt cgaggcgatg gcgggcgcga
     4981 tccgggcgca caccgcggag ctcgaggcca aggtgcgcga gcgcaccgac gccctgcacc
     5041 ggctggcgcg gatcgatccg ctcaccaacc tgaccaaccg ccgcggcatg agcgaaatgc
     5101 tggaggccga gatcatccgc gcggaacgcc agggcagccg cttcggcctg ctgtggctgg
     5161 atgtcgatca cttcaaggag atcaacgacc gccgcggcca tggtgccggc gacgaggcgc
     5221 tgacgaggct ggcgcgtgtc ctggaaggat gcctgcgccc ctacgaccgc gccgcacgtt
     5281 ggggtggcga cgaattcctc gtgctgctct cgccttgcga cgcggccacg ctggaggcga
     5341 tcgcccaccg cgtccgcgcc gacatcgagc acggcagcgc ggcctgcggc gaacgcgtca
     5401 cggtcaccat cggcgcctgc ctggcggaac cgggcgagac cctcggcgtg gtgctgcagc
     5461 acgccgacga tgcgctctat cgtgccaagg acgccgggcg caaccaggtc gtgatcgccg
     5521 cccgctgaaa ccgagcgccc cactccacca ggccgccgcc ggtgcgctcg agacggccgc
     5581 cccccgcggc atcgccttcc tcagcgctgg cagcgacccg cgatgccgag cccgccggag
     5641 agcgcgcggt cggtctcggc gatccactgc gcgatggcaa gcgccagccg ctcggtcgcc
     5701 gcacggaagg cgctcgcccc accgctcgca tcggccgtcg tggccggctc cgccacgacg
     5761 agcgaagcgc tggcgatgac cgcggcgccg cgcaccggca gcagggcaaa gcgagccagg
     5821 agacgagcct cgctgcgttc gcccgcggtg aagacctgct cgaactcgtc gagctggacc
     5881 tgcagccggc acccgccacc ctcgccggca ccgagcgtgc gctccagcac gagtccgagc
     5941 atggcgggag gtggagcggc ccagcggttg tcggcatagg cgcggcgacg ccctggatcg
     6001 acccgggcat cgcgataatg catcgtcgtc gtctgcagcc atgggggcga ggacaccgcc
     6061 acccgcgcgg gcgcgagccc gtgggcgagc gcggcgcgct cgccggtgcc gagatcgtag
     6121 gtcgccgccg cacgcggacg ctcggggagc acctggcagg cggccaccag cagcgcggtc
     6181 gcgatacacg cggacaggcg cggcaggacg cggtggcagt cgggatgacg tggctgcatt
     6241 cctttctccc ttcggctcat cggtcgctcc ccgcaaagcc ctcgtggccc gcctcaccgg
     6301 gcccgggcag aggacggtcg cgcccggtca gcaggagctg cggcgaggcg tcgagctcct
     6361 cgaccaggtg gccgaagcgt tgcgaggtgc tcgtgagctc accgagcagg acatcgagct
     6421 gcggcagcgt gcgctcgacg aggtcgtccc ctgcggcggc ggcgcggagc tccagacgcg
     6481 cggccgcctg atcgatgcgg acgatcaggc ggcgcagctc ggcaacggcg ggcacggctt
     6541 cggccgagcc gcgctcgagg ttcccgagca cggaggtgat gcgcgcgatg ttcccggcac
     6601 tcaaggcggc ccgcagcgcg gccagggtcg ccggcagttc cgccaccccg cgatcgacgc
     6661 ccgcggccgc tgactccagg ctggccagcg ccccctgcag gcgctcgccg agcgcctgga
     6721 actggtcgag ggcggcgagc gcccgcccgg agagcgcctc caccagcccg ggttgcaagg
     6781 cgatgcgcgg cacctcgcca tcggcggcag ccaagggccg gcggtccacc ccgcgatcgt
     6841 cgagctgcac gaaggccagg ccggtcacgc ccagcgtgcc gagcgcggcg gtggtgccgc
     6901 gggtcagggg cagatcggcg ggcaccgcga tgcgcaccag cagcgtgcgc aggtcctcgg
     6961 ggtcgatcgc gacggcgcgc acgctcccca ccgccatgcc gcggtagcgc acgcgcgact
     7021 gtgcgcccag gccgttgatg tcgccgcgtg cctcgagcac gatctcgcgc atcggcacgc
     7081 gctggcccga aaaccaccag atcgcgaaca cgacgccggc accgagcaag attgcgaaca
     7141 gcccggcggc aagcgcgtgg gcacggtttt ccatttcagc ctgctcctgc gccgcgcggc
     7201 tggccgcagc gcgcctgcag cccactgaaa aagcgggcga cgaaggggtg gtcgtaggcg
     7261 agcgtgtcgg cgagcgaggc gtggctgacg atgcggcgct cggcgagcac cgccacgctc
     7321 gtggccatct gcgccagtgt atcgagatcg tgcgtgacca gcaccacggt gagtccgagc
     7381 tcgcgatgca gcgcgcggat caggcggatg aagctcgcgc tgcggtcggg gtcgagcccg
     7441 gcggtgggct cgtcgagcag cagcaactcg ggctcgagcg cgagtgcgcg cgcgagcgcc
     7501 acccgcttga ccatcccgcc ggacagctcg gcaggcatca gtggcgcatg cgccggctcc
     7561 atctccacca tcgccagctt gagggcgacc aggcggtcga tctccggccg gctgatcacg
     7621 ccgagctcgc gcagcggaaa ggcgatgttc tgcgccacgc tgaacgccga gaacaaggcg
     7681 ccgtgctgga acaacacccc catgcggcgc tggcgggcga gccgcgcggc cgccccgccg
     7741 gcatcgagcg cctcgccgaa cacctccacc cggccggcgg cgggccggct cagcccgacg
     7801 aggtggcgca gcagtgtggt cttgccactg cccgagccgc ccacgagggc gacgatgtcg
     7861 cccgcgcgga tggtgaggtc cagcccctcg tgcacgatat tgcgcccgaa acgggtgacg
     7921 atgccttcga ggcagaccac ggcgtcgctc gtcgcccgct cgctcacagc ggcagcccga
     7981 tcgaacgggt ggcgatcgcg aacaccgcgt ccaccaggat caccaccgtg atcgccgcca
     8041 ccaccgaggc ggtggtgttc gcggacaggc tctcggtgtc gggcttcacc cgcaagccga
     8101 aatggcaggc cacgagcgcg atcaggaaac cgaacaccgc ccccttggcg agcccgatgt
     8161 agaggttggc caccggcacc gcatcgggca gcgcccgcag gaagaactgc agcccgatgc
     8221 cgagctccac ccaggccgac acccagccgc cgaaaagggc gatcgccgag gtccacaaca
     8281 ccagcagcgg catcgcgagg gaaagcgcca ccaccttggg cagcaccagg cgcaggctgc
     8341 gcgagacgcc catcgccgcg agcgcatcga tctcctcggt cacccgcatc accccgagct
     8401 gcgccgtcat cgccgaaccg gagcgccccg ccaccagcac cgacaccagc acggggccga
     8461 gctcgcggat gatgcccatg ccgaggatgt tgacgatgaa ggcgtccgcg ccgaaggtgc
     8521 gcagctgcag cgcggacaga taggacagca ccacaccgat caggaagccg accagggccg
     8581 agaccggcat cgccttgacg cctaccttgt ggaggtttgc cgagatctcc agcagcggcc
     8641 agtcgcgtgg atggtgcagc aggtggccga gatcgagcac gagacgaccg aggagcgcga
     8701 cgaagccgga aagatggcca gcgaatgcga acaggccctt gccgagcagc gccaccgcgt
     8761 cgagcgcgga agcgcgcggc ggctcgggca gcgcacgggc ggaggcgttc tcgatacggc
     8821 cgatgatggc gcgctgggcg tcatcggcct cgagcgccgc aggccagcgt ccgccccagg
     8881 cctgccacag cagcaaggcg ccgaagctgt ccatgcggcg caccgcgcgc agcgaccagc
     8941 gcgcgtcggg aggcaccgtg gccagcgccc ggcgcagcgt cgggaggcac tcgtcgagac
     9001 cgcgcagggt ccagtcgccg gcgaggcgga tgccctcggc tccggccgtg aagacctcgt
     9061 cgagcacacg tcctgccatc tgcgctcagg ccacaggcgt gcgcaggctg agcgcccgga
     9121 tgtagagcgc gcgcccgaga ccctgccact ggcgctgctc ctcgtcgagc gcggccgcgg
     9181 tgagcggcag cttctgcagc cagcccggcg cggcggtgag gctgaagccg cggccctcgc
     9241 gacgcagcgt gacgggcggc aggccgcgcg cgtcgcgggc gcggtggatc accaccgcca
     9301 ggcgcatgca ggcgatcagc agccagtcca cgctgccagg gtcgatcgag gcgacacgtt
     9361 cgagcttgcc acgatgcgcc agcaccaggc gcgccaggcg ggcctggtcc atgcgcgaga
     9421 aaccgggcat gtcggcgttg ccgacaatgt aggcgctgtg cttgtggtag ctcgagtgcg
     9481 ccaccgagat gccgatctcg tgcagcatcg ccgcccagcg caggaagcgg tggtcgtcgt
     9541 gctcgggatc ggcggtctcc ggctcgagct gggcgagcag gtggcaggcc gtgtcggcga
     9601 cctgctgggc ctggcgcagg tcgacgccgt agcgccgcac gaaggcgctt accgtggcgt
     9661 cgcgcagatc gtagtggtgg tagcgaccga gcaggtcgta gagcacgccc aggcgcaacg
     9721 cgccttcgga gaacaccatg tgctcgagct cgaactcctt gaagatcgcg ctcatgatcg
     9781 cgaagccgcc caggatgacg ggcaggcggt cgcccttgag gccggcgagg tcgatgttgt
     9841 ccaggctgcc ggcgcgcagc agcagcgcct tgagcttctc caggccctcg cgggtgatcc
     9901 cgctcgccga caggccgttc tgctcgagga tctccacgat cgccttggcg gtgccgctcg
     9961 aacccaccgc ctcttcccag ccggcctcgc ggtaggccgc cgagatcgtc tgcagctcgc
    10021 ggcgggcggc gagttcggcc tccttgaagg cgcgcttgtc gatgcggccc tccggaaagt
    10081 agcgcaggct gtagccgacg cagcccatgt agcgcgactc cagcagctgc gactcgaagc
    10141 tcttgccgat gatgaattcg gtcgaaccgc cgccgatgtc gaccaccagc tgctggcggt
    10201 gcggatccgg cagcgtgtgt gcgacgccga cgtagatcag gcgggcctcc tcgcggccgg
    10261 cgatgatctc gatcgggaaa ccgagcgcgg cctcggcgcg gatcaggaac tcgggcgcgt
    10321 tcttggccac gcgcaaggtg ttggtcgcca cggcgcgcac ccgggtgggc gggaagtcgc
    10381 gcaggcgctc gtggaagcgc tgcagggcgc cgaccccgcg ctgctgcgag gcgaggtcga
    10441 gcatcttgtc gggcgacagg ccggcggcca ggcgcaccgc ctccttgaga ccgtcgagcg
    10501 gatagatctg gtcgttgacg atgcgcccca cctgaagccg gaagctgttg gaaccgaggt
    10561 cgattgcggc gatcagatct tgcatggttt tgtcggcggc tggcacccgc ggggcgccgg
    10621 aaccctggat tctagcaccc cgcaaaaggc ttccgagccg cgccggcatg gcgccgggga
    10681 aggccccgcg ggcgcggccc gacgtcatca aaccgaaatg gaaatgtcac ataatcgcga
    10741 gcgaacttcc gcgcacagga atcccgaatg cccgtcgacc gcatgaccct gaactacccg
    10801 cccgagcact tcctcaaccg cgagctttcg ctgctgcaat tccagcgccg cgtgctggcg
    10861 caggccgccg accccaccgt gccgctgctc gagcgcctgc gcttcctgtg catcgtgtcg
    10921 agcaacctgg acgagttctt cgagatccgc gtctccggca tcaaggagca gatccgcatc
    10981 ggcagccgca agtcgggcga ggacgggatc gcgcccgtcg agctgctcga gcgcgtcagc
    11041 gacgaggtgc acgagatcat ctccgagcag taccagctgc tcaacggcga catcctcccg
    11101 gcactcgaga aggaaggcgt ggtcttcctg cgccgcagtc tgtggaccga ggcccagcgc
    11161 gcatggatcc acgactactt catgcgcgag gtcatgccgg tgctgacccc gatcgggctg
    11221 gacccggcgc atcccttccc acgcgtgctc aacaagagct tgaacttcgc cgtcgagctc
    11281 gaaggccgcg acgccttcgg gcgcgactcg ggcgcggcga tcgtgcaggc gccgcgcgcg
    11341 ctgccgcggg tgatccgcct gccgcgcgag ctgtgcgatc aggagtacgg cttcgtgttc
    11401 ctgtcctcgg tgctgcacgc ccacgtgggc gagctgttct cgggcatgaa cgtgctcggc
    11461 tgctaccagt tccgcgtcac gcgcaactcc gacctgttcg tcgacgagga agaggtcaag
    11521 gatctgcgcg cctcgctcaa gggcgagctg cagcaacgtc acttcggcga cgcggtgcgc
    11581 ctggaggtcg ccgacaactg ctccgaggag atggcgggtt ttctcctgca gcacttccac
    11641 ctcacccccg acgacctcta ccgcacgccc ggcatcgtga acctggtgcg cctcatgcag
    11701 gtccccgact gggtcgagcg cccggacctc aagtacgcac ccttcgtgcc cggcctgccc
    11761 aaggtgctcg acaaacgccg ccaggtcttc accgcgatcc gcggcggcga catcctgctc
    11821 caccacccct tccagagttt cgccccggtg atcgagatgc tgcgcgcggc agctgacgac
    11881 ccgcaggtgc tggcgatcaa gatgacggtg taccgcaccg gcaccgactc ggtgctgatg
    11941 gagcacctcg cccgcgccgc gcagaagggc aaggaagtca ccgtggtgct cgaactgatg
    12001 gcgcgcttcg acgaggaggc caacatcacg ctggccaacc gcctggaaga ggtcggtgcg
    12061 cacgtcgtgt atggcgtgtt cggctacaag acccacgcca agctgctgat gatcgtgcgc
    12121 cgcgaggaag acggcctgcg ccgttatgtg cacatgggca ccggcaacta ccacccgcgc
    12181 accacacgct tttacaccga cttcggcctg ctcacctgca acgcggagat cggcgaggac
    12241 gtcgccaccg tgttcaagca gctcaccggc ctgggcaagg ccaccgagct gcgccacctg
    12301 tggcaggcgc ccttcaccct gcagcccaac gtggtcgcgg cgatccggcg cgaggccgag
    12361 atcgcgcgcg cgggccggcg cggtcgggtg atcgcgaaga tgaacgcgct gctcgaaccc
    12421 gagaccatcg aggcgctcta cgaggcctcc caggccgggg tcgagatcga cctcatcgtg
    12481 cgcggcccgt gcgcgctgcg cccgggcgtg cccgggctgt cggacaacat ccgcgtgcgc
    12541 tcggtgatcg gccgcttcct cgagcaccac cgcatcttcc acttccacgc cgacggcgcc
    12601 gagcacatgt acctgtcgag cgcggactgg atggaccgca acttcttccg ccgcatcgag
    12661 atcgccttcc cggtgctcga cccgcgcctc aagcgccgcg tcatgaagga aggcctgcgc
    12721 ccctacctcg gggacaactg ccaggcctgg gacatgcagg cagatggcaa ataccgccgc
    12781 aagcacccgc gcagcatccg ccgcgcggcg cagctcatcc tgctgaagga gctcgcggcg
    12841 agcagctgag gcgtcgatac gggaaggcgt gctgcgcgag tcggcgatgg gggggcgccg
    12901 ctgaagcgct cgccggacca agcccgatgc cgcgcaagcc cgccgcaggg ccgggggcgg
    12961 cgctcacttg cgctgcgcgt cgatgacgcc gggcacctcg cgcaccagcg tgatcgcgcg
    13021 ctgcacctgc ttgatgccgc ccacctccat ggtgaagcgc atgtaggcgg tgcccttctt
    13081 ggtcagggtg ttgacggcga tcacgttgag cttctcgcgc gacagcacct cggagatgtc
    13141 gcgcagcagt ccctggcggt cgctcgcctg gacgttgatg tcgacctggt agagcgcgtt
    13201 gcgggtgtcg tgcgccttct cgccccactc ggccgggatc acgcgctcgg ggtgcttgcg
    13261 cgcgaggtcc tggaagtcgg cgcagtcgac gcggtggatc gagatgccgc ggccgcgcgt
    13321 gacgaagccc tcgatcgcat ccggcggcgc gggcttgcag cagcgcgaca gcgaggtcat
    13381 cagcttgccc acgccgacga tcaggatctt gtcctgattg tcgcccgagc gactgcggct
    13441 gaccacgaac tcgggttcgg cctccgccgc cggctcgggg gcgctgccct cgcgcagcgc
    13501 ggtctgcatc gcgcgcgggc ccacctcgcc acgcccggcg gccaggtaca gcgcctcggc
    13561 gttcttgaag ccgaggcgct cggccaggcc gtcgatgttg gcctggccgt ggccgtcgcg
    13621 ctgcatctcc ttggtgacga agctgcgccc gcgcgagagg atctcctcct cgtccagttg
    13681 tgcgaagtac tgcttgatct tgctgcgcgc acgcgaggtg gcgacgtagc cctggcgcgt
    13741 gtccagccag tcgcgcgagg ggccgccctc cttggcggtg acgatctcca ccgtctgccc
    13801 gctctccagc tgggtgttca ggggtatgag gtggccgtcg accttggcgc cgcggcagtg
    13861 gtggccgacg tcggtgtgca ggcggtaggc gaagtcgacc ggcgtggcgc cgcgcggcag
    13921 gtcgaccacc ctgccctgcg gcgtcagcac gtagagcgta tcgtcgagcg aggcgcgctt
    13981 gagctgctcc acccagtgcg ccgcatcggt gacctcgtcg cgccacgaca acaggctgcg
    14041 cagcagcgcg atcttctcgt cgtagtcgcc gccctgcctg gcgccctcct tgtagcgcca
    14101 gtgcgcggcc acgccgagct cggcgtgctt gtgcatgtcg aaggtgcgga tctgcacctc
    14161 gagcgcgcgc ccgtcgcccg ccagcacggc ggtgtgcagc gactggtagt tgttgccctt
    14221 gggcttggtg atgtagtcgt cgaactcctt cgcgatcggc tgccacatct ggtgcacgat
    14281 acccagcacc gtgtagcagt ccttgatctc gggcaccagc acgcgcagcg cgcggatgtc
    14341 gaagacctgc gagaagtcga gccgcttctt gcgcatcttg ttgtagatgc tgtagatgtg
    14401 cttggcgcgg ccggtgatct cggcctcgat tccgaccgcg gcgagctcgc tcttcaggcg
    14461 ctcgatggag tcgcggatga actgctcgcg ctccacccga cgctcgtcga gcatcttcgc
    14521 gatgcgcttg taggtgtcgg gctcgatgaa gcggaaggag agatcttcca gctcccactt
    14581 gatctgccac acccccagcc ggttggcgag cggcgcgtag atgtccaggc tctcgcgcgc
    14641 gacctcgacg cgggcctcgc ccggcaggtc ggtgtagaag cgcagggtct gggtgcgcga
    14701 ggccagccgc accagcacga cgcggatatc ctcgaccatc gccagcagca tcttgcgcag
    14761 gacttcggtc tgcgcgcgga tctcgggcgc gctcgcgttg gtggtgaggc gggtgagcac
    14821 gcgcaggccg ttgagcttgt gcaggccgcg caccagcgcg gtgatcgccg gaccgaagcg
    14881 cgcctcgatc tcctcggcgg gggcctcgag ttcctcccag gctgcgaaca acagcgcggc
    14941 cacgcgcgtc tcggcgtcca aacgcagcga ggccgcgatc aacgcggcac cgatcgcgtg
    15001 cgtccaggtc ggctcgccgg tgccgagcac cttgccctcg tagaggtcgg cgatccattc
    15061 gagcgcggct tcgatgtgct cgcgttcggt cggctccagg ccggccgcga gcagatcgat
    15121 cgggggggcg gtgtcgccct gggagatggc gtgggtgacg gataccatgg ctgcaattat
    15181 cccacatcgg gcagtccatc gtgctgcatg cccgtgctcg cagccccttc gccgggtgcg
    15241 actccccggg gtcagccgcg ccggcgcggc gtctcgaagg tggtgagcat gccgcgcagc
    15301 agggcgacct cctcgcgctc gaggcgcacg cggttgaaca agcgccgcat gcgcggcaac
    15361 aggcgcttgg ggttggcagg atcgtggaag ccgctggcgg tgaccgcacg ctcgaggtgc
    15421 gccatgaagc cctcgaagtc ggcatgcgtg gccggctcgg gctgcgggtc ggcgggcgcg
    15481 gcggcgtcgc cgagcgcggc catgcgcagc tcgtaggcca gcacctgcac cgccgcgccg
    15541 aggttcaggg aggagaaccc ggcatcggtg gggatggtca ccggcaggct gcacaaggcg
    15601 gcctcctcgt tggtgagccc gctggtctcg ttgccgaaca ccagcgcgac ggcgccgtcc
    15661 tgcgtgcgcc gcacgagctc cagcgccgcg tcgcgcgcct gcaggcgcgg cagcgagagt
    15721 tcgcgccggc gcgcggtcag cgccgcggaa aagaccgtgt cggcgagcgc ctcctccagc
    15781 gtgccgacca cccgcgcggc gtccagcagg tcggccgcgc ccgaggcgcg cgcggtggcg
    15841 acctcgtcgg gaaaggactc gggcgcgacc agccagagct cgcgcaggcc catggtcttc
    15901 atggcgcgcg cggcggcgcc gatgttgccg ggatgggtgg tacgggacag cacgacgcgg
    15961 atgcggtcga gcgcgagggg gcgattcata ttaaaattcc gcgttttccg aatttgccgg
    16021 gctgcctgcc cggaacgtgg cccggcggtc gcgcggcggc tcgatgaagc gggcggcggc
    16081 gcgcgcccga cgcacgtggc aggccccgat tctttaaccg agaccgctcc gcatgcatcc
    16141 caccctgaac atcgccgtca aggccgcccg tcgcgccgcg tccgtcatca accgcgcgtc
    16201 gacccagctc gatctgctga ccgtccagtc caagtcgccc aacgacttcg tcaccgaggt
    16261 cgaccgcgcc gccgaacagg cgatcatcga ggtgctgcgc gacgccttcc cggggcacgg
    16321 gattctagca gaggagtccg gcgagtccgg acccgagagc gaatacacct ggatcatcga
    16381 cccgctcgac ggcaccacca atttcatcca cggcatgcca cagtacgcgg tctcgatcgc
    16441 gcagacgaag aacggcgtgc tcgagcacgc ggtggtctac gaccccaaca ccaacgagat
    16501 gttcaccgct tcgcgcggcg ccggcgcctt cctcaacgac cgccgcatcc gcgtctcgcg
    16561 ccgcacccgc ctcaacgagg cgctgatcgg caccggcttc cccttccgcc agttcgacca
    16621 cgtcgacgcc tacctggcga tgttcaagga gctcacccag aagaccgccg gcatccgccg
    16681 cccgggcgcc gcctcgctcg acctcgccta cgtggcgtcc ggccgcttcg acggcttctg
    16741 ggagatgggc ctgtcgccct gggacatggc ggccggcgtg ctgctgatcc aggaggccgg
    16801 cggcctggtg tccgacctgt cgggcgaagc caactacctc accaccggca acctggttgc
    16861 cggcacgccg aagatcttcg gccagctgct gccgatcatc caggcctggc gcccggccaa
    16921 cctgcgggcc tgaggaccga gaaaacgcgg cccgctagcc ggaacccggc gcgcgcaggg
    16981 tgagcgtgtc gccgcgctgg gtaaactcca ggtgacgcag cgccgacatg ccgagcagca
    17041 cctcgtcgcc ctgcatgccc gggttcaggt ggccggccac gtcactgaac tcgaagggcc
    17101 cgaaggccag acgcgggatg cgtgtctggc gcaccacgac cgcgccgttg gcggtcagca
    17161 ccgaggccgg cgcgcccgcc tgcaggccca gttccgcggc cagccgcgcc ggcacagaga
    17221 cctgggtcgc gccggtgtcg agcagcaggc gcaccggacg gccgttgatc tcgcccggca
    17281 gcaggtagtg cccggcgcgg ttgcgctgca gcaccagctc gctggcgccg ggggcgacca
    17341 cgagattacg gttggggtcc tcgcggcgct cgagcgcgcc gtcgaagctc acccagccca
    17401 gcgcgagaaa ggcgagcgtc gccagcaggc ccagcctgcg gccgatgcgg cgggtgtcgg
    17461 cgtcgggtgt gttcaagtcg gtctttcctt cgatccacgg tgataatcct gcctttgctg
    17521 acgacagcac ggcaaccggg aggtttcttc agttggcaat ccgcaagccg cccttcggcg
    17581 acacagccat ccgccccgcc gcgctgagcg aggccgagat cggcgcccac actccgatga
    17641 tgcagcagta cctccggatc aagcagcagc atccggacac cc
//
LOCUS       DAOQNN010000023        17485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1229_length_17485_cov_9.506713, whole genome shotgun sequence.
ACCESSION   DAOQNN010000023 DAOQNN010000000
VERSION     DAOQNN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 17485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17485
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1229_length_17485_cov_9.506713"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /gene="xseA"
                     /locus_tag="PKV97_02885"
     CDS             complement(<1..837)
                     /gene="xseA"
                     /locus_tag="PKV97_02885"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNB04850.1"
                     /translation="MPANPLLPANGAATPAAQVLSVSELNRMARELLESALPLMWVGG
                     EISNLVRAASGHVYFTLKDASAQVRCAMWRNRAQLLAFRPENGMRVEARALVTLYEAR
                     GDYQLSVEALRPAGIGSLFEAFNRLKAKLAAEGLFDEAGRRALPRYPRALGIVTSPQA
                     AALRDVLVTLRRRAPHLPVVLYPAPVQGADAPARLLEAVRCAGRRAAEDGVDVLLLVR
                     GGGSIEDLWAFNDEALARTLRACPLPVVCGVGHETDFTIADFAADLRAPTPSGAAELA
                     SAG"
     gene            1200..1808
                     /locus_tag="PKV97_02890"
     CDS             1200..1808
                     /locus_tag="PKV97_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB04851.1"
                     /translation="MFAIIQAVGWPIWPLILASVIAVALIIERSITLRRSRIVPPGLL
                     DKVLDDLRQIGPSAEMAERVAAHSPLGRVLAAGLRNVHSPREVMKESIEETGRAVVHD
                     LERFLTTLGTIASISPLMGLLGTIIGMIDIFASQGVSGANPAQLAQGISIALYTTGLG
                     LIIAIPAMIFWRHFRALIDSFVIDMEQQAIKLVEVSHGERRA"
     gene            1819..2244
                     /locus_tag="PKV97_02895"
     CDS             1819..2244
                     /locus_tag="PKV97_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB04852.1"
                     /translation="MNFRRGSRNGEPEINLIPLIDVVLVIIIFLMLTTTFSKVSGLEI
                     KLPTAEAEGSETEAREIVVAVTAAGEVLVNRQAVGERSVEAIAAALGKSAPADGDTPP
                     VVVINADARTPHQSVVDVMQAAQRAGLPHITFATQSSAQ"
     gene            2279..3286
                     /gene="lpxK"
                     /locus_tag="PKV97_02900"
     CDS             2279..3286
                     /gene="lpxK"
                     /locus_tag="PKV97_02900"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNB04853.1"
                     /translation="MAAQAPAFWQRRGLLAQALRPLSLLFGFLARRRRALTQPQRLPV
                     PVIVVGNVAVGGSGKTPVVDWLAGVLRVAGHHPGIVSRGHGGTASAQGGLALVPADGD
                     PGRYGDEPVLLARLTACPLVVGQDRPAAARALLRLHPECDVLIADDGMQHYHLARDLE
                     IAVVDAATLGNRLLLPAGPLREPLARLAEVDLVLAHGELDAELRAAIGGVPVFPMRLF
                     GEEVVALADPACRLPLAALRGRRVHAVAGIGRPQRFFDQLAAAGLEVVAHAFPDHHRF
                     VAADLVFGDASPILMTAKDAVKCRAFAPADCWEYPVRAQIGSGAAERILEKLEDGRTS
                     A"
     gene            3267..3458
                     /locus_tag="PKV97_02905"
     CDS             3267..3458
                     /locus_tag="PKV97_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNB04854.1"
                     /translation="MDARLLEILVCPLCKGPLDYLKDKQELVCKADRLAYPIRDGIPV
                     MLDEEARAMSAEEVDALRK"
     gene            3455..4240
                     /gene="kdsB"
                     /locus_tag="PKV97_02910"
     CDS             3455..4240
                     /gene="kdsB"
                     /locus_tag="PKV97_02910"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNB04855.1"
                     /translation="MSGGMSTDFRIVVPARYASTRLPAKPLADIGGKPMIVRVLERVR
                     HAGASETWVATDHEGVREVVQAAGGAVVMTRPDHPSGTDRLAEVALARGWADEDIVVN
                     VQGDEPLIDPEIVAAVARALAADGEAAIATAAHAIHDPVEVFNPNVVKVVCDAAGRAL
                     YFSRAPIPWARDAWGDGARDLPAGLPVLRHVGLYAYRVGFLRRYAALAPSPLEHWEAL
                     EQLRALWHGYRIRVLELAQAPAAGVDTQEDLERVRAIFAAGGV"
     gene            4328..4981
                     /gene="adk"
                     /locus_tag="PKV97_02915"
     CDS             4328..4981
                     /gene="adk"
                     /locus_tag="PKV97_02915"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNB04856.1"
                     /translation="MRLILLGPPGAGKGTQANFIKEKFGIPQISTGDMLRAAVKAGTP
                     LGLEAKKVMDAGGLVSDDIIIGLVKDRLQQEDCKSGYMFDGFPRTIPQADAMKDAGVP
                     IDFVLEIDVPDAEIVERMSGRRAHLASGRTYHVKYNPPKVAGKDDVTGEDLVQRDDDK
                     EETVKKRLDVYHSQTKPLVEYYSKWAASGDAKAPKVRKISGLGAVDAITANAFAALK"
     gene            5120..6373
                     /locus_tag="PKV97_02920"
     CDS             5120..6373
                     /locus_tag="PKV97_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNB04857.1"
                     /translation="MRQQNLLYAHSGGVTAVINASAAAVVAAARASGRVGKVFAAWRG
                     IVGVLEEALIDTAGLDEAALVRLARTPGGAFGSCRFDLPHDDGGAAMDRLFAVFDAHD
                     IGWLLYNGGNGSMDAVARIQAEAARRGHALVCVGVPKTVDNDIVGTDCCPGFGSAARY
                     LATSMLEAGLDTASMVGSKGSVFVMEVMGRNTGWLAAATALAAQGDPDRAPHIVLVPE
                     ARFDEAAFLARVREIVERLGYCAVTVAEGIRRPDGSLLMEKSRDARGYVQLGGAGLAV
                     ARRINERLGYKFHWAIPDYLQRSAGHWLSATDRAQAEAVGRVAVEYALAGQGGTMPAI
                     RRLQDAPYRWDVVAVDTAAVANLERSLPDGFVRADGLHVTTAACDYMRPLIEGEIPPP
                     VAGGLPDYGRWDWAPVARRLAAWQA"
     gene            6454..>7592
                     /locus_tag="PKV97_02925"
     CDS             6454..>7592
                     /locus_tag="PKV97_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HNB04858.1"
                     /translation="MRNLNLVVAGVLFLGLLWGLEGLPDRAGAGLALLATIAWLWLTE
                     ALHVSVTALLVPLLAAASGVLDFPEALLQFADPVVFLFLGGFALAAGLQRQGLDRWMA
                     GQVLRRAGASFGRAVRWLFWLTAFLSMWISNTATAAMMLPLALGLLAPLPLDEHRRTW
                     MYVLLGVAFSANIGGIGTLVGSPPNAIAAAAVGMDFAEWMSWGLPLVLVMMPLMEIGL
                     RLVLRPRLDVAVTPPPPPGGWTPAQRKMLALFGFTVLLWVFGAPLGAALGFAQGYDAA
                     VALFALVLLHGLRLAGWQDVERSTEWGVLLLFGGGLTLSKALAASGAAAWLATQLGGP
                     LAALPAWGCIALMVLFALLLTEVTSNTASAALLIPLFLGLAPHVER"
     assembly_gap    7593..7692
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7693..10220)
                     /locus_tag="PKV97_02930"
     CDS             complement(<7693..10220)
                     /locus_tag="PKV97_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB04859.1"
                     /translation="MKNAASFAIRILTAIAIAAVVAFAQYWIWQQLNRSTEFIGTNQS
                     IKGFAYNGFQRDQSPLKGTYPTRAELASDLDLLGRYSDGLRTYGVNDMPELLDLAGER
                     DMLVTAGAWIDARPESNAREVAALIEAARKMRHIERVMVGNEAILRGDVTVDELIVYL
                     DEVRKAIRKPVSTAEPWHVWLRYPELAKHVDYITVHLLPYHEGLPVDKAVEYAFQRYD
                     EVARAHPRKKIVVGEVGWPSRGPTIDAAIPSLDNQARFVREFLAHPRTARIDYFLMEA
                     IDQPWKVDVEGWAGPYWGMFNADREPKYQLEGVVERDPHWSHKASNAAALAFIPMMLA
                     AFFLPGWSIGGRLFLAALIQACISTLIIGINVPVEYYLTQRDLIGLVLLIGATCMTAA
                     VLLSHGFEFGEVLFKKKWARRFTPLPPHPPEQQPFVSIHLACYNEPPEMVIATIDSLA
                     QMNYQNFEVLILDNNTRDEALWKPLERRCAELGPRFRFFHLANWPGFKAGALNYGLKV
                     TDPRAEVVGVVDADYVVDPDWLACLVPHFDQPEVAVVQAPQAHRDWEGQPFKRMCNWE
                     FDGFFRIGMHHRNERNALIQHGTMTMVRRRALEEVGGWSEWCICEDTELGLRLIEKGY
                     DTRYIDHILGRGLTPSGFAAIKSQRFRWAFGAMQILKAHLPHMIGRSTLNLAQRYHFL
                     TGWFAWLGDALQLVFAFGSLLWTLGILLFPKAFGLPVVSLALPIFGFMIFKAALGPIL
                     YRRTMDCPWKDILGASILSVGLAHAIARGVFAGLVKKKGVFVVTPKGWRGGGALAFFN
                     PIREEIGMLVALLMGAATLVAQRGANNLETQLWVGILCLQCIPYV"
     gene            10525..10959
                     /locus_tag="PKV97_02935"
     CDS             10525..10959
                     /locus_tag="PKV97_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04860.1"
                     /translation="MPTNLLKYGVARPLLAGLALAALALWLRHGVLEAGVLPRDCTAA
                     DAPALACLFKQAFVHSFLDQRIGWVSLVAGVLAFVRASRGLAWAGWLCGVAGLVLYSY
                     DPAAVGALLALLVLVRAHQQHGQGQREPGEQPGDRLGVGRLA"
     gene            complement(10852..12474)
                     /locus_tag="PKV97_02940"
     CDS             complement(10852..12474)
                     /locus_tag="PKV97_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-glucan synthase"
                     /protein_id="HNB04861.1"
                     /translation="MIFAPRTNRSQLWLALLIASTTMLLLLAAMLWLQTRPVRMHDLH
                     LAEGEKLRCVSYAPYHRPGQTPLLEDTRIEREQIAADLAALAKITDCVRLYSIDQGLE
                     HVPELARPLGLKVLLGAWIGYEKTKNARELERAIALANANADVVRALIVGNEVLLRRE
                     RTEDEMRELIRHAKAHAKVPVTYADVWEFWTRHDSLANEVDFVTVHILPFWEDEPVDI
                     ELALQHVAETRRHVGEHFAGKKILIGETGWPSAGRQREESKPSRVNQARYIREFVHRA
                     HAEGWDYNLIEAIDQPWKRRLEGTVGGFWGMLDADSLEPKFPLAGAVAEREGVAGPVG
                     GAAVGGVLAFLLATTGRRTRCLRASAIAAAGTLGGMITVLHWEHAHLAYRDALEWSLL
                     GGVAALGALLPLTLARWHGEPVPATGRAARILRHGEPLRAKAGALGLLRGLLLFAAAV
                     AALLLWVDPRYRDFPTLLYLVPAVVLGVVGWWRADTTRSEITLALVILIGVVARWSSE
                     PANPQAVAWLLTGLALALPVLLVRSHQHEQRE"
     gene            complement(12624..15518)
                     /gene="gcvP"
                     /locus_tag="PKV97_02945"
     CDS             complement(12624..15518)
                     /gene="gcvP"
                     /locus_tag="PKV97_02945"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNB04862.1"
                     /translation="MTDTLLSAPLARLEQRDAFVHRHLGPDAGEIGRMCASIGVADVD
                     ALIAQTVPAGIRLASPLPLAGAMPEHEALARLKAIAGHNVVKKSLIGQGYYGTHVPAV
                     VLRNVLENPGWYTAYTPYQAEISQGRLEALLNYQQMVIDLTGLELANASLLDEATAAA
                     EAMTMARRVSKSKSNVFFIDEACFAQTIDVVRTRAHYFGYELVYGKAAEAGSHDCFGA
                     LLQYPNARGEVVDLTAPIAELKAKGAVVAVASDLMALVLLKSPGAMGADIALGSAQRF
                     GVPMGFGGPHAAFFATRESYVRSMPGRIIGVSKDARGKTALRMTLQTREQHIRREKAN
                     SNICTSQVLLANLSGFYAVYHGPQGLRTIAARIHRLAAVLAAGLREAGFGLHDRAFFD
                     TVEVEVGARAPTILRAAEEAGFNLRSVSGTTLGLSVDETTTREDIATILGCFGASTDV
                     SAIDARVAAAGGALPAELLRSDAVLAHPVFNTHHTEHEMLRYLKKLQNRDLALDHSMI
                     SLGSCTMKLNATSEMIPITWPEFANLHPFAPREQAAGYLEMIEGLADYLRAVTGFPAI
                     SMQPNSGAQGEYAGLVAIARYHASRGEANRKICLIPKSAHGTNPATAQMCGMQVVVVE
                     CDDNGNVDVADLKAKAEKHAADLAALMITYPSTHGVFEESIREICTIVHQYGGQVYMD
                     GANLNAQVGLTSPATIGADVSHMNLHKTFCIPHGGGGPGMGPIGLAAHLAPFMADHVI
                     QPTGGAGRPNLGQGAVSAAPFGSASILPISWMYITMMGGEGLKQATEVAILNANYLAA
                     RLKDHYPVLYTGSKGRVAHECILDIRPIKATTGISEVDIAKRLMDYGFHAPTMSFPVA
                     GTIMVEPTESEDLGELERFVAAMIAIRDEIRQIEAGNWPAEDNPLKHAPHTQADFLGD
                     WKRPYSREQAVFPLPWVAENKFWPSVNRIDDVYGDRNLFCACVPMGDYAE"
     gene            complement(15521..15907)
                     /gene="gcvH"
                     /locus_tag="PKV97_02950"
     CDS             complement(15521..15907)
                     /gene="gcvH"
                     /locus_tag="PKV97_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNB04863.1"
                     /translation="MSNVPSTLKYTKSHEWIRVEDDGTLTIGVTDHAQEALGDIVFLE
                     LPEAGRALSAGETCAVIESVKAASDIYAPVAGEVIANNQDAIDAPESVNADAYSAWLF
                     KLAPANAADVAALMDAAAYEAEIAQA"
     gene            complement(16029..17120)
                     /gene="gcvT"
                     /locus_tag="PKV97_02955"
     CDS             complement(16029..17120)
                     /gene="gcvT"
                     /locus_tag="PKV97_02955"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNB04864.1"
                     /translation="MTIAAKQTPLHAAHVAAGARMVDFAGWDMPVNYGSQIEEHHAVR
                     RDAGMFDVSHMLALDLAGPDATTWLRGLLANDVAKLKDNGKALYSCMLNERGGVIDDL
                     IVYRFSDADYRIVVNAGTADKDVAWMRQRIAATGANVTLQARRDLAMVAVQGPRAIER
                     TALSLPELSTANGVPAPFFGARVGDLLIARTGYTGEDGLEIAVPASRAEAMWNALAAA
                     GVRPCGLGARDTLRLEAGMNLYGQDMDDHVSPLNAGLSWTVDMKDEARDFLGRAALQA
                     NPASQRLLGLILEDKGVLRSHMKVFTSGGEGETTSGSFSPTLEKSIAFARVPLATQPG
                     EAVEVDIRGKRLKARTVKLPFVRNGKALV"
     regulatory      complement(17327..17413)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
BASE COUNT         2456 a         5705 c         6411 g         2813 t
ORIGIN      
        1 gccggcgctg gcgagctcgg cggcgccgct cggcgtgggt gcgcgcaggt cggcggcgaa
       61 gtcggcgatg gtgaagtcgg tctcgtggcc cacgccgcac accaccggca gcgggcaggc
      121 gcgcagggta cgcgccaggg cttcgtcgtt gaatgcccac aggtcctcga tgctgccgcc
      181 gccacgcacc agcagcagca catcgacgcc atcttcggcg gcgcgccggc cggcgcagcg
      241 gaccgcctcg agcaggcgcg cgggagcgtc ggcgccctgc accggcgcgg ggtagagcac
      301 caccggcaga tgcggcgcgc gccggcgcag cgtcaccagc acgtcgcgca gcgcggcggc
      361 ctgcggcgag gtgacgatac cgagcgcgcg cgggtagcgc ggcagcgcgc gccggccggc
      421 ctcgtcgaag aggccttcgg cggcgagctt ggccttgagc cggttgaagg cctcgaacag
      481 gctgccgatg ccggccgggc gcagcgcctc gacgctcagc tggtagtcgc cgcgcgcctc
      541 gtacagggtg acgagcgcgc gcgcctcgac gcgcatgcca ttctcggggc ggaaggcgag
      601 cagctgcgcg cggttgcgcc acatcgcaca gcgcacctgg gcggaggcgt ccttgagggt
      661 gaagtagaca tggccggagg cggcgcggac gaggttggag atctcgccgc ccacccacat
      721 caggggcagc gcggattcga gcagctcgcg cgccatacgg ttgagttcgg agacgctgag
      781 gacctgcgcg gcgggcgtcg cggcgccgtt tgcgggcaag agggggttcg cgggcatggc
      841 cggattgtag cggaatccac aggctttcga ccttgtcaag ggtgtgtttg caagtccttg
      901 atcgataagg ggtgattctg caagttattg attcgtaaag gttcgatggc ttgcacaatt
      961 ttgcatcgga gtgagggaaa gcctgccgtt gcgggctttg cgggcaggat tccgaaatcg
     1021 tccacaaact tatccacagc ttctgtggat tgccgcgtgc gctcccgatc tcctcgtgcg
     1081 caggccatgc ccgtgcccgc agatgcccgg tgcccgcgtg cgcagcgtga tgcgtgcggc
     1141 ttgccgggcg gggggcgcgg cgctagagtc tcgcctttcc aatcgagagg tgagcccgcg
     1201 tgttcgcgat catccaggcc gttggctggc cgatctggcc cctgattctg gcgtcggtca
     1261 tcgcggtggc gctcatcatc gagcgctcga tcaccctgcg ccgttcgcgc atcgtgccac
     1321 cggggctgct cgacaaggtc ctcgacgatc tgcgccagat cgggccctcg gccgagatgg
     1381 ccgagcgcgt cgcggcgcac tcgccgctcg gccgcgtact cgccgccggc ctgcgcaacg
     1441 tgcacagccc gcgcgaggtg atgaaggagt cgatcgagga gaccgggcgc gcggtggtgc
     1501 acgacctcga gcgcttcctc accacgctcg gcaccatcgc ctccatcagc ccgctgatgg
     1561 gcctgctcgg caccatcatc ggcatgatcg acatcttcgc ctcgcagggc gtgagcggcg
     1621 ccaacccggc ccagctcgcc cagggcatct cgatcgcgct gtataccacc gggctgggcc
     1681 tcatcatcgc cattccggcg atgattttct ggcgccactt ccgcgcgctg atcgacagct
     1741 tcgtgatcga catggagcag caggcgatca agctcgtcga ggtctcccac ggcgagcgcc
     1801 gggcgtaagg gaggggcgat gaacttccgt cgcggcagcc gcaacggcga gcccgagatc
     1861 aacctgatcc cgctcatcga cgtggtgttg gtgatcatca tcttcctgat gctgaccacc
     1921 accttctcca aggtctccgg gctggagatc aagctgccca ccgccgaggc cgagggcagc
     1981 gagaccgagg cgcgcgagat cgtggtcgcg gtaaccgccg ccggcgaggt cctggtgaat
     2041 cgccaggcag tgggtgagcg cagcgtcgag gcgatcgccg ccgcgctcgg caagtcggcc
     2101 ccggccgacg gcgacacgcc gccggtggtg gtgatcaacg ccgacgcgcg caccccgcac
     2161 cagagcgtgg tggacgtgat gcaggccgcg cagcgcgcgg ggctgccgca catcaccttc
     2221 gccacccagt cgtcggcgca gtgaggtcct ccggcccgcg ccgccccgga gactgaacgt
     2281 ggctgcgcag gccccagcgt tctggcagcg tcgcggcctg ctcgcgcagg ccttgcgtcc
     2341 gctctccctg ctgttcggtt tcctcgcccg ccgccgccgc gcgctcacgc agccgcagcg
     2401 cctgccggtg ccggtgatcg tggtcggcaa cgtcgcggtc ggcggcagtg gcaagacgcc
     2461 ggtggtcgac tggctggccg gggtgctgcg tgtcgctggc caccaccccg gcatcgtcag
     2521 ccgcggccat ggcggtacgg cgagtgcgca gggcgggctc gcccttgtgc ccgcagacgg
     2581 cgaccccggc cgctatggcg acgagccggt gctgctcgcc cgtcttaccg cttgcccgct
     2641 ggtggtgggg caggaccgtc ccgccgctgc gcgcgcgctg ctgcgcctgc atcccgagtg
     2701 cgacgtgctc atcgccgacg acggcatgca gcactaccat ctcgcaagag acctggagat
     2761 cgccgtggtg gacgcggcca cgctgggcaa ccgcctgctg ctgcccgccg ggccgctgcg
     2821 cgagccgctc gcgcgcctcg ccgaagtcga tctggtgctg gcccacggcg agctggatgc
     2881 cgagctgcgc gcggcgatcg gcggcgtgcc ggtgtttccg atgcggctct tcggcgaaga
     2941 ggtggtcgcg ctcgccgacc ctgcgtgtcg cctcccgctc gcggcgctgc gtggtcgccg
     3001 ggtgcacgcg gtggccggga tcggcaggcc gcagcgcttc ttcgaccagc tcgccgccgc
     3061 cggcctggag gtggtcgccc acgccttccc cgatcatcat cgcttcgtcg ccgccgatct
     3121 cgtcttcggc gacgcgtcgc cgatcctgat gaccgcgaag gatgcggtaa aatgccgggc
     3181 tttcgcgcct gccgactgct gggaataccc ggtgcgggcg cagatcggtt ccggcgccgc
     3241 cgaacgcatc ctggagaaac tcgaagatgg acgcacgtct gcttgaaatc ctcgtctgcc
     3301 cgctgtgcaa ggggccgctc gactatctga aggacaagca ggaactggtg tgcaaggccg
     3361 accgcctcgc ctatccgatc cgcgacggca tcccggtgat gctggacgag gaggcgcgcg
     3421 ccatgagcgc cgaggaagtc gacgccctgc gcaaatgagc ggcggcatgt ccaccgactt
     3481 ccgcatcgtc gtccctgcgc gctacgcctc gacccggctg cccgccaagc cgctcgccga
     3541 catcggcggc aagccgatga tcgtgcgtgt gctcgagcgc gtgcggcatg ccggcgccag
     3601 cgagacttgg gtcgccaccg accacgaggg cgtgcgcgag gtcgtgcagg ctgcgggcgg
     3661 tgccgtggtg atgacgcgcc cggaccatcc gagcggcacc gaccgcctcg ccgaggtcgc
     3721 gctcgcgcgc ggctgggccg acgaggatat cgtggtgaat gtgcagggcg acgagccgct
     3781 gatcgacccg gagatcgtcg ctgcggtggc gcgcgcgctc gccgccgacg gcgaggctgc
     3841 catcgccacc gccgcgcacg ccatccacga tcccgtcgag gtcttcaacc ccaacgtggt
     3901 caaggtggtg tgcgatgccg ccgggcgcgc gctgtacttc tcgcgcgcgc ccatcccctg
     3961 ggcccgcgac gcctggggcg acggtgcgcg cgacctgccc gcagggctgc cggtgctgcg
     4021 ccatgtcggc ctgtacgcct accgcgtcgg cttcctgcgc cgctatgcgg cgctcgcgcc
     4081 ctcgccgctg gagcactggg aggcgctcga gcagttgcgc gcgctctggc acggctatcg
     4141 catccgcgtg ctcgaactcg cccaggcacc ggcggccggg gtcgacacgc aggaggacct
     4201 ggagcgggtg cgcgcgatct tcgccgccgg aggggtttga ccacccggcg cgcgtcggtt
     4261 aagtttcggc tgcttattcg ataaggaaac ggccattttc gatggccggc cattcacggg
     4321 agggagtatg cgtctgattc tgttgggacc gccgggagcc ggcaagggta cgcaagccaa
     4381 cttcatcaag gagaagttcg gcattccgca aatctccacc ggcgacatgc tgcgcgccgc
     4441 ggtgaaggcg ggcaccccgc tgggcctgga ggcgaagaag gtcatggatg ccggcggcct
     4501 cgtgtccgac gacatcatca tcggcctggt gaaggaccgc ctgcagcagg aagactgcaa
     4561 gtccggctac atgttcgacg gcttcccgcg caccatcccg caggccgacg cgatgaagga
     4621 cgccggcgtg ccgatcgact tcgtgctcga gatcgacgtg ccggacgccg aaatcgtcga
     4681 acgcatgagc gggcgccgcg cccatctggc ttcgggccgc acctaccacg tcaagtacaa
     4741 cccgcccaag gtcgcgggca aggacgacgt caccggcgaa gacctggtgc agcgcgacga
     4801 cgacaaggaa gaaaccgtca agaagcgcct cgacgtctac cactcgcaga ccaagccgct
     4861 ggtcgagtac tacagcaagt gggcggcctc gggcgacgcc aaggcgccca aggtgcgcaa
     4921 gatctccggc ctcggcgcgg tggatgcgat caccgcgaac gcctttgcag cgctgaagta
     4981 ggcagcaccc cggccgggca tgcccggcct catccggcgc ggccgcgccc cccacggggc
     5041 gacggccgcg cgtgcttttg aactcgccgc aatctccgcc cggcgcggcg cgcggcaacg
     5101 ctcgaacgag gcccggccga tgcgccagca gaaccttctg tatgcccatt ccggtggcgt
     5161 aaccgccgtc atcaacgcca gcgccgcggc ggtcgtcgcg gccgcgcgcg cctcgggtcg
     5221 ggtcggcaag gtgttcgccg cgtggcgcgg catcgtcggc gtgctcgagg aggccctgat
     5281 cgataccgcc ggcctggacg aggcggcgct cgtccgcctg gcgcgcaccc cgggcggggc
     5341 cttcggctcc tgccgcttcg atctgccgca cgacgacggc ggcgcggcga tggatcggct
     5401 gttcgcggtt ttcgacgcgc acgacatcgg ctggctgctc tacaacggcg gcaacggctc
     5461 gatggacgcg gtcgcgcgca tccaggccga ggccgcgcgg cgcggccatg cgctggtgtg
     5521 cgtgggcgtg cccaagacgg tggacaacga catcgtcggt accgactgct gtcccggctt
     5581 cggctcggcc gcgcgctacc tcgccacttc gatgctggag gcggggctgg acaccgcgtc
     5641 gatggtgggc agcaagggca gcgtcttcgt catggaggtg atggggcgca acaccggctg
     5701 gctggccgcg gccaccgcgc tcgccgcgca gggcgacccg gaccgcgccc cgcatatcgt
     5761 gctggtaccg gaggcgcgct tcgacgaagc ggccttcctc gcccgcgtgc gcgagatcgt
     5821 cgaacggctg ggctattgtg cggtgacggt ggcggagggc atccgccgac cggatggcag
     5881 cctgctgatg gagaagtcgc gcgatgcgcg cggctacgtc cagctcggcg gcgcgggtct
     5941 ggcagtggcg cggcggatca acgagcgcct cggctacaag ttccactggg cgatccccga
     6001 ctacctgcag cgctcggccg gacactggct gtcggcgacc gaccgcgcgc aggccgaagc
     6061 ggtcggaagg gtggcggtgg agtacgcgct cgccggccag ggcgggacca tgcccgcgat
     6121 ccgccgcctg caggatgcgc cctaccgctg ggatgtggtc gcggtggaca ccgccgcggt
     6181 ggccaacctc gagcgcagcc tgccggacgg tttcgtgcgc gccgacgggc tgcacgtgac
     6241 cacggcggcc tgcgactaca tgcgtccgct gatcgagggc gagattccgc cgccggtggc
     6301 gggcggcctg cccgactacg ggcgctggga ctgggccccg gtggcgcgcc ggctggcggc
     6361 gtggcaggcc tgacgcggat cggcgtgcaa cgccgcgtac gtacgggtgt gtgctcgagc
     6421 cgatagcgcc actgcgcagg ggtggatcaa ttgatgcgga acctcaatct agtcgtcgcg
     6481 ggcgtactct tcctcggcct gctctggggc ttggagggtc tgccggaccg cgccggagcc
     6541 gggcttgcgc tgctcgccac gatcgcctgg ctgtggctga ccgaagccct gcacgtgagc
     6601 gtgaccgcgc tgctggtgcc cctgctcgcc gccgcgagcg gagtgctgga ttttcccgag
     6661 gccttgctgc aattcgccga cccggtagtc ttcctcttcc tcggcggctt cgcgctcgcc
     6721 gccggcctgc agcgccaggg gctggaccgc tggatggcgg gccaggtcct gcgccgcgcg
     6781 ggggccagct tcgggcgcgc ggtgcgctgg ctgttctggt tgaccgcctt cctgtcgatg
     6841 tggatcagca acaccgcgac cgcggcgatg atgctgccgc tggcgcttgg cctgctcgcc
     6901 ccgctgccgc tcgacgaaca tcgccgcacc tggatgtacg tgctgctcgg cgtggccttc
     6961 tccgccaaca tcggcggcat cggcaccctg gtcggcagcc cgcccaacgc gatcgccgcg
     7021 gcagcggtgg gcatggattt cgccgaatgg atgagctggg gtctgccgct ggtgctggtg
     7081 atgatgccgc tgatggagat cggtctgcgc ctggtgctgc gaccgcggct cgacgtggcg
     7141 gtgacgccgc caccgcctcc cggaggatgg actccggcgc agcgcaagat gctcgcactc
     7201 ttcggcttca ccgtgctgct gtgggtgttc ggcgcgcccc tcggcgccgc gctcggcttt
     7261 gcccagggct acgacgccgc ggtggcgctg ttcgcgctcg tgctgctgca cggcttgcgg
     7321 ctggcgggct ggcaggacgt ggagcgcagc accgagtggg gcgtgctgtt gctcttcggc
     7381 ggcgggctca cgctgagcaa ggcgctcgcg gccagcggcg cggcggcctg gctcgccacc
     7441 cagctcggtg gaccgctcgc cgcactgccg gcctggggct gcatcgcgct gatggtgctg
     7501 ttcgcgttgc tgctcaccga ggtcaccagc aacaccgcga gtgcagccct gctgatcccg
     7561 ctcttcctcg gtctcgcccc gcatgtcgag cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnacgtaggg gatgcactgc aggcacagga tgccgaccca gagctgggtc
     7741 tcgaggttgt tggcgccgcg ctgggcgacc agggtggcgg cgcccatcag cagggcgacc
     7801 agcatgccga tctcctcgcg gatcgggttg aagaaggcga gcgcaccgcc gccgcgccag
     7861 cccttgggcg tcaccacgaa cacacccttc ttcttcacca ggccggcgaa cacgccgcgc
     7921 gcgatcgcat gtgcgaggcc caccgacagg atcgaggcgc cgaggatgtc cttccacgga
     7981 cagtccatgg tgcgccggta caggatgggg ccgagcgcgg ccttgaagat catgaagccg
     8041 aagatcggca gcgcgagcga caccaccggc aggccgaagg ccttggggaa gagcaggatg
     8101 cccagggtcc acagcaggct gccgaaggcg aacaccagct gcagcgcgtc gcccagccag
     8161 gcgaaccagc cggtgaggaa gtggtagcgc tgggcgaggt tgagggtgct gcgcccgatc
     8221 atgtgcggca ggtgggcctt gaggatctgc atcgcgccga aggcccagcg gaagcgctgg
     8281 ctcttgatcg cggcgaagcc cgagggggtg aggccgcggc cgaggatatg gtcgatgtaa
     8341 cgcgtgtcgt agcccttctc gatcaggcgc aggccgagct cggtgtcctc gcagatgcac
     8401 cactcggacc agccgccgac ctcctcgagt gcgcgccggc gcaccatggt catggtgccg
     8461 tgctggatca gcgcgttgcg ctcgttgcgg tggtgcatgc cgatgcggaa gaagccgtcg
     8521 aactcccagt tgcacatgcg cttgaagggc tggccctccc agtcgcggtg ggcctgcggg
     8581 gcctgcacga cggcgacctc gggctggtcg aagtgcggca ccaggcaggc cagccagtcg
     8641 ggatcgacca cgtagtcggc gtcgacgaca cccaccacct cggcgcgcgg gtcggtgacc
     8701 ttgaggccgt agttgagcgc gccggccttg aagccgggcc agttggcgag gtggaagaag
     8761 cggaagcgcg gaccgagctc ggcgcagcgg cgctcgagcg gcttccacag ggcctcgtcg
     8821 cgggtgttgt tgtcgaggat caggacctcg aagttctggt aattcatctg cgccaggctg
     8881 tcgatggtgg cgatcaccat ctcgggcggc tcgttgtagc aggccaggtg gatggacacg
     8941 aagggctgct gctcgggcgg gtgcggcggc agcggggtga agcggcgtgc ccatttcttc
     9001 ttgaacagca cctcgccgaa ctcgaagccg tgcgacagca gcaccgccgc ggtcatgcag
     9061 gtggcaccga tcagcagcac cagaccgatg aggtcgcgct gggtgaggta gtactcgacc
     9121 ggcacgttga tgccgatgat cagcgtcgag atgcaggcct ggatcagcgc ggcgaggaag
     9181 aggcggccgc cgatgctcca gccgggcagg aagaaggcgg cgagcatcat cgggatgaag
     9241 gcgagcgcgg cggcgttgct ggccttgtgc gaccagtgcg ggtcgcgctc gaccacgccc
     9301 tcgagctggt acttgggctc gcggtcggcg ttgaacatgc cccagtaggg cccggcccag
     9361 ccttcgacgt ccaccttcca cggctggtcg atcgcttcca tcaggaagta gtcgatgcgt
     9421 gcggtgcgcg gatgggcgag gaactcgcgc acgaagcgcg cctggttgtc gagcgagggg
     9481 atggcggcgt cgatggtcgg gccgcggctc ggccagccca cttcgccgac cacgatcttc
     9541 ttgcgcgggt gtgcgcgcgc gacctcgtcg tagcgctgga aggcgtactc caccgccttg
     9601 tccaccggca ggccctcgtg gtacggcagg aggtgcacgg tgatgtagtc gacgtgcttg
     9661 gcgagttccg gatagcgcag ccagacgtgc cagggctcgg cggtcgagac cggcttgcgg
     9721 atcgccttgc gcacctcgtc gagatagacg atgagctcgt cgaccgtgac gtcgccgcgc
     9781 aggatggcct cgttgccgac catgacgcgc tcgatgtggc gcatcttgcg tgccgcctcg
     9841 atcagggctg cgacttcgcg cgcgttggac tcggggcggg cgtcgatcca ggcgccggcc
     9901 gtgaccagca tgtcgcgctc gccggcgaga tcgagcagct cgggcatgtc gttgacgccg
     9961 taggtgcgca ggccgtcgga gtagcgtccg agcaggtcga ggtcggaggc gagttcggcg
    10021 cgcgtggggt aggtcccctt gagcgggctc tggtcgcgct ggaagccgtt gtaggcgaag
    10081 cccttgatgc tctggttggt gccgatgaac tcggtgctgc ggttgagctg ctgccagatc
    10141 cagtattgcg cgaaggcgac caccgcggcg atggcgattg cggtgaggat gcggattgcg
    10201 aaggaggcgg cgttcttcat cgtggttcct gggcgccgtc ctgcaggtac ggcgcggatg
    10261 tattgcgagg gcctcgtggg ccgcgtgtcg gggtgacacc gggcggctgc ggcgggcagg
    10321 ccatgaaggc gctgcgccgc cacgatcgcc gacccggccg cactgtgaca aaatgcgcgc
    10381 gcccggcagg gcgtccgccc ttcacaccga cggcgcgcat tttattcgcc atttcagccc
    10441 cttacgagcc gctcggggtg gcaatttgca acactttgca cgtcttgtcg gcgtgtgctg
    10501 attttgaagg gaaagcgtat cccaatgcca actaatttgc tcaaatatgg cgtcgcccgt
    10561 ccgctccttg ccggactcgc gctcgccgca ctcgcgctct ggttgcgcca cggcgtgctc
    10621 gaggccgggg tgctgccgcg cgactgcacg gccgccgatg cgcccgcgct ggcctgcctg
    10681 ttcaagcagg ccttcgtgca cagcttcctc gaccagcgca tcggctgggt gagcctcgtc
    10741 gcgggcgtac tcgcctttgt gcgtgccagt cgcggcctgg cctgggcggg ctggctgtgt
    10801 ggagtggccg gcctggtgct gtacagctac gacccggccg cggtcggcgc cctactcgcg
    10861 ctgctcgtgc tggtgcgagc gcaccagcag cacgggcagg gccagcgcga gcccggtgag
    10921 cagccaggcg accgcctggg ggttggccgg ctcgcttgac cagcgcgcga ccacgccgat
    10981 gaggatgacc agcgcgaggg tgatctcgct gcgcgtggtg tcggcgcgcc accagccgac
    11041 gacaccgagc accaccgccg gcaccaggta gagcagggtg gggaagtcgc ggtagcgcgg
    11101 atcgacccac agcagcagcg cggccaccgc ggctgcgaag agcagcaggc cgcgcagcag
    11161 gccgagcgcg ccggccttgg cgcgcagggg ttcgccatgc cgcaggatgc gcgcggcgcg
    11221 gccggtggcg ggcacgggct cgccatgcca gcgcgcgagc gtgagcggca gcagggcgcc
    11281 gagcgcggcg acaccgccga gcaggctcca ctccagcgca tcgcggtagg ccaggtgggc
    11341 gtgttcccag tgcagcaccg tgatcattcc gccgagtgtg cctgcggcgg cgatcgcgct
    11401 tgcgcgcagg cagcgcgtgc gtcggccggt ggtcgcgagc aggaaggcga gcacgccgcc
    11461 gaccgcagcg ccgccgaccg gaccggccac gccttcgcgc tcggccaccg caccggcgag
    11521 cgggaacttg ggctccaggc tgtcggcgtc gagcatgccc cagaagccgc ccaccgtgcc
    11581 ttcgaggcgg cgcttccagg gctggtcgat ggcctcgatg aggttgtagt cccagccttc
    11641 ggcatgcgcg cgatggacga actcgcggat gtagcgcgcc tggttgaccc gggagggctt
    11701 cgattcctcg cgctggcggc ccgcgctcgg ccagccggtc tcgccgatga ggatcttctt
    11761 gccggcgaag tgctcaccga cgtggcgccg cgtttcggcc acgtgctgga gcgcgagctc
    11821 gatgtcgacc ggttcgtcct cccagaaggg caggatgtgc acggtgacga agtcgacctc
    11881 gttggcgaga ctgtcgtggc gggtccagaa ctcccacacg tcggcgtagg tgaccggcac
    11941 cttcgcatgc gccttggcgt gacggatgag ttcgcgcatc tcgtcctcgg tgcgctcgcg
    12001 gcgcagcagg acctcgttgc cgacgatgag cgcacgcacg acgtcggcgt tggcgttggc
    12061 gagcgcgatc gcgcgctcga gttcgcgggc gttcttcgtc ttctcgtagc cgatccaggc
    12121 gccgagcagc actttcagac cgagcggacg ggcgagctcg gggacgtgct cgaggccctg
    12181 gtcgatcgag tacaggcgca cgcagtcggt gatcttcgcc agcgcggcga ggtcggcggc
    12241 gatctgctcg cgctcgatgc gggtgtcttc gaggagcggg gtctgccccg ggcgatgata
    12301 gggggcgtag gacacgcagc gcagcttctc gccctcggcg aggtgcaggt cgtgcatgcg
    12361 caccgggcgc gtctgcagcc acagcatggc ggcgagcagc aggagcatgg tggtgctggc
    12421 gatgagcagg gcgagccaga gctggctgcg gttggtgcgc ggagcgaaga tcaccgtgtc
    12481 cggttccttc caggttcggg tggtggtggt ggtggggagg gctggcggca aaaaaagagc
    12541 cacacccgcg ggcgtggctc ttgctgccga ggctggcgga ttctaccagc gctcggggcg
    12601 tggttcgccg ctgcgggcgg cgctcactcg gcgtagtcgc ccatcggcac gcaggcgcag
    12661 aacaggttgc ggtcgccata gacgtcgtcg atgcgattga cgctgggcca gaacttgttc
    12721 tcggccaccc agggcaacgg gaacaccgcc tgctcgcgcg agtagggccg cttccagtcg
    12781 ccgaggaagt cggcctgggt gtgcggcgcg tgcttgagcg ggttgtcctc ggccggccag
    12841 ttgccggctt cgatctggcg gatctcgtcg cggatcgcga tcatcgcggc gacgaagcgc
    12901 tcgagctcgc cgaggtcttc cgactcggtc ggctcgacca tgatcgtgcc tgccaccggg
    12961 aacgacatgg tcggcgcgtg gaagccgtag tccatcaggc gcttggcgat gtcgacctcg
    13021 gagatgccgg tggtcgcctt gatcggacgg atgtcgagga tgcactcgtg cgcgacccgg
    13081 cccttgctgc cggtgtagag caccgggtag tgatccttca ggcgtgcggc caggtagttg
    13141 gcgttgagga tcgccacctc ggtcgcctgc ttgaggcctt cgccgcccat catcgtgatg
    13201 tacatccacg agatcggcag gatgctcgcc gagccgaagg gcgcggccga gactgcgccc
    13261 tggcccaggt tgggccggcc ggcgccgccg gtgggctgga tcacgtggtc ggccatgaag
    13321 ggcgcgaggt gcgccgccag gccgatcggc cccatgcccg gaccgccgcc gccgtgcggg
    13381 atgcagaagg tcttgtgcag gttcatgtgc gacacgtcgg cgccgatcgt cgccggcgag
    13441 gtcaggccaa cctgcgcgtt gaggttggcg ccgtccatgt acacctgacc tccgtactgg
    13501 tggacgatgg tgcagatctc gcggatcgac tcctcgaaca cgccgtgggt ggacgggtag
    13561 gtgatcatca gtgcggccag gtcggcggcg tgcttctccg ccttggcctt gaggtcggcg
    13621 acgtcgacgt tgccgttgtc gtcgcactcg accaccacca cctgcatgcc gcacatctga
    13681 gcggtcgccg ggttggtgcc gtgggcggac ttcgggatca ggcagatctt gcggttggcc
    13741 tcgccgcggc tggcgtggta gcgcgcgatc gccaccaggc cggcgtactc gccctgcgca
    13801 cccgagttcg gctgcatgct gatcgccggg aagccggtga ccgcgcgcag gtagtcggcc
    13861 aggccctcga tcatctcgag gtagccggcg gcctgctcgc gcggcgcgaa cgggtgcagg
    13921 ttggcgaact ccggccaggt gatcgggatc atctcgctgg tggcgttcag cttcatcgtg
    13981 cacgagccga gcgagatcat cgagtggtcg agcgcgaggt cgcggttctg cagcttcttg
    14041 aggtagcgca gcatctcgtg ctcggtgtgg tgcgtgttga acaccgggtg cgcgagcacg
    14101 gcgtcgctgc gcagcagttc cgccggcagg gcgccgcctg cggccgcgac gcgggcgtcg
    14161 atcgcgctga cgtcggtgga ggcgccgaag cagccgagga tcgtggcgat gtcctcgcgg
    14221 gtggtggttt catccaccga cagaccgagc gtggtgccgg agacgctgcg caggttgaag
    14281 ccggcttcct cggccgcgcg caggatggtg ggcgcgcgcg cgccgacctc gacctcgacg
    14341 gtgtcgaaga aggcacggtc gtgcaggccg aagccggcct cgcgcaggcc ggcggcgagc
    14401 accgcggcga ggcggtggat gcgcgcggcg atggtgcgca ggccctgcgg gccgtgatag
    14461 acggcgtaga agccggagag gttggccagc agcacctgcg aggtgcagat gttggagttc
    14521 gccttttcgc ggcggatgtg ctgctcacgg gtctgcagcg tcatgcgcag cgcggtcttg
    14581 ccacgcgcgt ccttggagac gccgatgata cggccgggca ttgagcgcac gtagctttcg
    14641 cgggtcgcga agaaggcggc atgcgggccg ccgaagccca tcggcacgcc gaagcgctgc
    14701 gccgaaccga gcgcgatgtc ggcgcccatg gcgccggggc tcttgagcag caccagggcc
    14761 atcagatcgc tggcgaccgc gaccacggcg cccttggcct tgagttcggc gatcggggcc
    14821 gtgaggtcga cgacctcgcc gcgtgcgttc gggtactgca gcagcgcgcc gaagcagtcg
    14881 tggctgccag cctcggcagc cttgccgtag accagctcgt agccgaagta gtgcgcacgg
    14941 gtgcgcacca cgtcgatggt ctgcgcgaag caggcctcgt cgatgaagaa cacgttcgac
    15001 ttcgacttcg atacgcgccg ggccatcgtc atcgcctcgg cggcggcggt ggcctcgtcg
    15061 agcagcgagg cgttggcgag ctccaggccg gtgaggtcga tcaccatctg ctggtagttc
    15121 agcagggctt cgaggcggcc ttgggagatc tcggcctggt agggcgtgta ggcggtgtac
    15181 cagcccgggt tctcgagcac gttgcgcagc acgaccgcgg ggacgtgggt gccgtagtag
    15241 ccctgaccga tcagggactt cttcaccacg ttgtggccgg cgatggcctt caggcgggca
    15301 agcgcctcgt gctcgggcat cgcgccggcg agcggcagcg gcgacgcgag gcggatgccg
    15361 gcaggcacgg tctgcgcgat cagggcgtcg acgtcggcga cgccgatgct cgcgcacatg
    15421 cgcccgatct cgcccgcatc ggggccgagg tggcggtgga cgaaggcgtc gcgctgctcg
    15481 aggcgggcga gcggggcgga aagcagggtg tcggtcatgg tcaggcctgg gcgatttccg
    15541 cttcgtaggc tgcggcgtcc atcagcgcgg cgacgtcggc ggcgttggcc ggcgcgagct
    15601 tgaacagcca ggcggagtaa gcgtcggcgt taacgctctc gggggcgtcg atcgcgtcct
    15661 gattgttggc gatcacttca cctgccacgg gggcgtagat gtcggaggca gccttgaccg
    15721 attcgatcac cgcgcaggtt tcgccggcgg acagcgcacg gccggcttcc ggcagttcga
    15781 ggaagacgat gtcgccgagg gcctcctggg cgtggtcggt gacgccgatg gtcagggtgc
    15841 cgtcgtcctc gacgcggatc cattcgtggg acttggtgta cttgagggtg ctgggaacgt
    15901 tgctcatggt gctctccggt cgggaagatg gggtgtgcgg gtgaagaaat gcagtgttgc
    15961 gtccggctcg tcggtgatgt gcggctgcga catgaagggc ggctcgatgg ccgccctcgg
    16021 acatcgggtc agacgagggc cttgccgtta cgtacgaagg gcagcttgac ggtgcgtgcc
    16081 ttcaggcgct tgccgcgaat gtccacctcg acggcttcgc ccggctgcgt cgcgagaggg
    16141 acgcgtgcga aggcgatcga cttttccagc gtgggcgaga agcttccgct ggtggtctcg
    16201 ccttcgccgc cggaggtgaa gaccttcatg tgcgagcgca gcacgccctt gtcctcgagg
    16261 atgaggccaa gcaggcgctg actggcaggg ttggcctgca gcgcggcgcg accgaggaag
    16321 tcgcgcgcct cgtccttcat gtccaccgtc cacgacaggc cggcattcag gggggaaacg
    16381 tggtcgtcca tgtcttggcc gtagaggttc atgccggctt ccagccgtag ggtgtcgcgc
    16441 gcgcccaggc cgcagggacg cacaccggcg gcggcgagcg cattccacat cgcctcggcg
    16501 cggctcgcgg ggacggcgat ctccagtccg tcctcgccgg tgtagccggt acgtgcgatc
    16561 agcaggtcgc ccacgcgagc gccaaagaag ggggcgggca cgccgttggc ggtggagagc
    16621 tccggcaggg acaaggcggt gcgctcgatc gcgcgcggcc cctggacggc gaccatcgcc
    16681 aggtcgcggc gcgcctgcag ggtcacgttg gcgccggtcg cggcgatgcg ctggcgcatc
    16741 caggcgacgt ccttgtcggc ggtgccggcg tttacgacga tgcggtagtc ggcatcgctg
    16801 aagcggtaga cgatcaggtc gtcgatcacg ccgccgcgct cgttgagcat gcaggagtag
    16861 agcgccttgc cgttatcctt gagcttggcg acgtcgttgg cgagcagacc gcgcagccag
    16921 gtggttgcgt cggggccggc gaggtcgagg gcgagcatgt gcgagacgtc gaacatgccg
    16981 gcgtcgcggc gcacggcgtg atgctcctcg atctgcgaac cgtagttcac cggcatgtcc
    17041 cagccggcga agtcgaccat gcgtgcgccg gcggcgacgt gggcggcgtg gaggggggtc
    17101 tgtttggcag cgatagtcat cgcgaaggcg ctccgtcagg gtgaacgaga aaggggcggg
    17161 tacggtttgt caccgagcac gccccatctg tccttttacc tgagagattt tcccgtgatc
    17221 gcgtggcatc tgcagacata tatgtatgcg atgttacggc tgacgacggt gtgccccttc
    17281 ggtggatacg ccggcgcctt gcgcccgtac cgctctccag acttttgctg cgcacgcggt
    17341 ccttttgcct gagcggttcc gggggttgcg ccttcggcga ctccatcgag gagttctctc
    17401 ccgcgtgtcg cgcggacgat atcacttgcc ccacggtgcc gcaaccgcga cggatgcgtc
    17461 cgcgcgcgcc atcgtcgcga agctt
//
LOCUS       DAOQNN010000024        16964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1290_length_16964_cov_8.859069, whole genome shotgun sequence.
ACCESSION   DAOQNN010000024 DAOQNN010000000
VERSION     DAOQNN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 16964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16964
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_16964_cov_8.859069"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKV97_02960"
     CDS             complement(<1..444)
                     /locus_tag="PKV97_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNB04865.1"
                     /translation="MSAHRGSTDLDFIVPGDPAQRTGGYLYDARIVEALRHLGWTVTV
                     HGLPGRFPDADPTARDALHDTLATLPAGRRVVIDGLALGGLPEVAIEHAGRLALVALV
                     HHPLAEERGLDPVLRRCLLASERAALAAVRLAITTSAFTARRLLDF"
     gene            complement(441..839)
                     /locus_tag="PKV97_02965"
     CDS             complement(441..839)
                     /locus_tag="PKV97_02965"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNB04866.1"
                     /translation="MYSLTVREHIMIAHSFRGEIFGPAQRVHGATYLVDACFQRSELD
                     ADGLVVDIGLAAATLKAVLAEFNFRNLDELEEFRGRNTTTEFMARVIFDRLAATIRAG
                     RLGPGAQGLERLKVSLHESHVAWASYEGAL"
     gene            complement(870..1868)
                     /locus_tag="PKV97_02970"
     CDS             complement(870..1868)
                     /locus_tag="PKV97_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HNB04867.1"
                     /translation="MSSTNLAFWTVRPGYGELRPAPLRPPADGELRVRNLFGAVSRGS
                     ESLVFRGEVPESEYERMRAPFQEGDFPGPLKYGYIGVGVVEDGVGTAATALRGRTVFC
                     LHPHQQRYVVPAGAVVPLPAGVPAARAVLAANLETAINACWDGAPALGDRIAVVGAGV
                     VGSLVAWLCARLPGVELELIDTDPGRAGLAAALGLVHRLPEQARGNCDLVFHASGNPA
                     GLVRALELAGQDATVVEMSWYGRRSVELPLGAAFHARRLRLQSSQVGRLPPPRSPRWD
                     YRRRMELALALLVDPRLDALISGETDFTDLPALMQRLAEAPAGALCERIRYASPST"
     gene            1982..2746
                     /locus_tag="PKV97_02975"
     CDS             1982..2746
                     /locus_tag="PKV97_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNB04868.1"
                     /translation="MGKGRKGSMAMEQGGSCVRRGGLPRLWLDLAGGFGGLVALVALA
                     SSTLALPLAAAWAAGAVLLAIAALIRHFWSDPRRGLGPANRVTLARALLVALLAGMLA
                     APAWVDAHPVQVAALAALALLLDGVDGWVARRWRCESDFGARFDMELDAFLILVLCVH
                     LLAMDKAGPWVLAIGAMRYVFVAAMRPWPWLERPLRESRRRKLVCVWQVACLLLCLLP
                     AIGGALATALLVLALALLAWSFAVDVRWLYHTRPQS"
     gene            2789..3406
                     /locus_tag="PKV97_02980"
     CDS             2789..3406
                     /locus_tag="PKV97_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB04869.1"
                     /translation="MESRRRFPRMLAAAVLAGVLGAAPAWAEPRSYTIDPEHFAIGFQ
                     VEHLGYADIIGLFLKASGRFEYDEAARTLKSGRVVVAADSVFSNHEARDRHVRDSDFL
                     DARKHPEIVFEASGFEAGAERDGRLHGKLAGRLTLLGQTHPVVLDVTLNKAASYPFGH
                     RKHTLGISARTTIERSRWGMDYGVSRGMVGDAVALRFELEAMRDD"
     gene            complement(3456..4535)
                     /gene="moaA"
                     /locus_tag="PKV97_02985"
     CDS             complement(3456..4535)
                     /gene="moaA"
                     /locus_tag="PKV97_02985"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNB04870.1"
                     /translation="MKVIPIQQAASPLAPAAPAPVAAPGAALVDRRARSVRDLRISVT
                     DRCNFRCVYCMPREVFGDDYAFLPRRQLLSFEEILRVARLFVARGVRKIRITGGEPLL
                     RKDVDRLIGMLAALDGVEVTLTTNGVLLPKLARRLKDAGLHRVTVSLDALDDALFRRM
                     NDADYPVEKVLEGIEAAKVVGLGPIKVNMVVKRGTNDVDIEPMAAHFRNSGHILRFIE
                     FMDVGASNGWKMDEVLPSREIVERIDRLFPLEPVDPNYEGEVAERWRYKDGAGEIGVI
                     SSVTQAFCSSCTRIRLSTEGQLYTCLFAQQGHDLRALLREGADDARIDAAIAQVWQGR
                     EDRYSEIRTAETAALRKIEMSYIGG"
     gene            4790..7219
                     /locus_tag="PKV97_02990"
     CDS             4790..7219
                     /locus_tag="PKV97_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB04871.1"
                     /translation="MADLVPLPATALRKRCDPACFSFDTTESLADHAGDLGQERAVEA
                     IRFGLAMGHTGYHVFVLGDAGSGKHATTFRLLRERAAREDVPPDLCYLHNFDEPLKPR
                     LLTLPAGRGAALRTRMQTFIRDLGPALGAALDSDAHVERVESLQSAHKAREEGALREL
                     GKACNAEGISLLQTPEGFVFAPTKDGEPLSPEAFEALPEDERKAIEARIGAWSDRLED
                     LLNDFPGWRKSVRESIDRAEREVLGPAVAHLLREVREAHEDLPEVLAFLDAVERDVID
                     SAVRGTMLEEEDDEAPEPEENTRYHRYQVKLLVDHSGSQGAPVVFENNPGYGNLIGRI
                     EHIVQQGNQVSHFNLIRAGALHRANGGYLVLDAERLLSQPFAWEGLKRSLRAGEIRIE
                     PPAEAQGWSGALTLEPEAVPSALKVVLIGDREVYYLLMEHDPEFADLFKVAADFNDDI
                     LRTPENECEYARLMALLARGAKLLPVERSGVARLVEEASRLAEDSGRLSLNTRALSDL
                     MREADFHARQKGLAATTRAEVEEALAARARRANRYPTRVLESILDGTTLISTTGERAG
                     QINGLVVIELAGELFGYPVRITATVRMGEGDVVDIERETELGGAIHSKGVLILSAFLA
                     ARYARHQPLSLSASLVFEQSYAPVEGDSASLAELCALLSALTQVPIQQRFAITGSVNQ
                     FGEVQVIGGVNEKIEGFFDLCSARGLDGTQGVVIPAASVRHLMLREDVVQAAAEGRFH
                     IHAVKTVDEAMTVLTGVEAGEPDAKGVIPKGTLNHKIATVLAEMTAARHAYSDADGAA
                     GARQHRRRHGV"
     gene            complement(7242..8624)
                     /locus_tag="PKV97_02995"
     CDS             complement(7242..8624)
                     /locus_tag="PKV97_02995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNB04872.1"
                     /translation="MNQPTSNPRRRFLTAAGGLATAAVAGPALAQSAPPAASTERSLP
                     FLIGRATDTLLPPGKQRRVVICGGGWGGVTAAKYLRQLAPQLDVVLLERNPVFFSCPM
                     SNKWLVDQVDTRYLTHDYLAVAEKYAYRFIQTEILEIEREGKRVITAAGAIHYDYLIV
                     APGIRYNYEAWFGNDRRMAEATRARFPAAYIPSAEHFHLKNALQSFKGGDLVMTLPPP
                     PQRCPPSPYERACLIASMFKQKKIPGRIYILDHKDGARPIGPGFRSTFEELYKDIITY
                     VPDARIEEVDPFKRRIRTEAGDFDFQHAILMAPHQAGDLAWKAGLVPRPGAGNPSGGW
                     ADVDPLFLHDRTDKEVFVIGDAVGFVSPQFGFYPKAGHVANRHARIVAYYISEREAGR
                     EPDYRLPDNLCFMMVNIAPQEGISVQFDYKVNAQGIIEQTQIDYNERTAATISQDFGW
                     AQTIYEDMFS"
     gene            complement(8764..9378)
                     /locus_tag="PKV97_03000"
     CDS             complement(8764..9378)
                     /locus_tag="PKV97_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNB04873.1"
                     /translation="MKLRLALLILVLAALLASGIAPYDRGVWWAEVAPVLIALPILLA
                     SAQRFPLTPLTYGLIAFFSLILILGGAYTYARVPVGFVVQDWFELARNPYDRFGHFFQ
                     GVTPAILGRELLLRTSPLRPGKWLFALLTLSCTGISALYELVEWASAELWAGGAVEFL
                     GTQGDPWDAQADMLMALLGAIIAQIVFGRLHDRQLARLAAQPST"
     gene            9598..10167
                     /locus_tag="PKV97_03005"
     CDS             9598..10167
                     /locus_tag="PKV97_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNB04874.1"
                     /translation="MKHRPLMLAASVLALLAQVGPVAAADAPSAPVEPIAALDVPRYM
                     GSWYEIARYPNWFQKKCVGDSRADYSLLESGEVRVVNRCREADGSVSEAVGVARQIGG
                     ADSPRLEVRFAPAWLSFLPIVWGNYWVLDLDPDYRLVAVGEPSREYLWILSRTPTVEA
                     RAYAELLERLAARGFDVGRLVRGEQGGGG"
     assembly_gap    10228..10327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10524..13433
                     /locus_tag="PKV97_03010"
     CDS             10524..13433
                     /locus_tag="PKV97_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HNB04875.1"
                     /translation="MLLASLVLLPFLGSVLAALMPQDARNRESWLAIGVAVGGLAIAA
                     ALFPQVAAGEVVRAEFAWLPAEGLDFVLRVDGLAWVFSMLVLAIGLLVVLYARYYMSP
                     EDPVPRFFAFLLAFMGSMLGVVLSGNLVQLVVFWELTSLTSFLLIGYWHHRADARRGA
                     RMAFTVTATGGLCLMAGVLVLGHIVGSYDLDVVLAAGDQIRGHELYLPALVLVGLGAL
                     TKSAQFPFHFWLPHAMAAPTPVSSYLHSATMVKAGVFLLVRLWPALAGTPEWTWIIGG
                     AGVCTLLIGAFVAIFQHDLKGLLAYSTISHLGLITVLIGIGTPLAIVAAIFHILNHAT
                     FKASLFMAAGIIDHETGTRDLRVLRGLRHKLPITATLAIVASAAMAGVPLLNGFLSKE
                     MFFAETLHVGGDRNWWMSYAAVAMGMFSVAYSLRFISVFFGTPAQGLPRDPHEPPRWM
                     RFPVELLVLACLVVGVAPGVSIEPLLHLAVVATLGEDAPAYDLAVWHGFNLPLLMSVC
                     ALVGGMLLYGVLRRHFDLARRERVPLLHRLNGAQAYETTMLQLTRGAEWLLRRVGTRR
                     LQPQLMLMMLALLLIPLLLAGAPPAWTVIPVRGFDPIFAGMWLIGAGCALAAAWMAKY
                     HRLAALALVGGTGIVTAATFLWLSAPDLALTQLMVETVTTVLILLGLRWLPPRIESIA
                     GPVQPPWQVWTRRTRDLALAVGGGLALAALTHAVLVHPASDTIADFFLLNALGGGGGS
                     NVVNVLLVDFRSFDTLGEITVLAVVALTVYALLRRFRPAPESAGIPPQQRFEGDPASR
                     QSPAEQAGSGYLLVPGIYLRLLLPFMGVIAVYFFMRGHNLPGGGFVAGLIFAVAILVQ
                     YMLAGTVWVESRLNLRPHRWLGFGLLLACTTGLGAWWFGHPFLTTHTAHVEWPLVGEL
                     HLPSAFVFDLGVFAVVVGTTMLMLVALAHQSLRAHRHPGEDLTADGQGGAEGRRI"
     gene            13433..13774
                     /locus_tag="PKV97_03015"
     CDS             13433..13774
                     /locus_tag="PKV97_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HNB04876.1"
                     /translation="MELVLAISVGVVFGAGIWLILRPRTFQLITGLLLMSYAVNLFIF
                     AMGRLWVDRPPITPTPAIDPAQFADPLPQGLVLTAIVIGFATTALYLVMMIGARGLSG
                     SDHVDGEEPRE"
     gene            13771..15396
                     /locus_tag="PKV97_03020"
     CDS             13771..15396
                     /locus_tag="PKV97_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HNB04877.1"
                     /translation="MTVLPWLNHLIIVPVLLPLIVGALMIPINQKRHGLKFGLGLASG
                     MLLWAVAVVLLVLADGGYWPGGIGVYLAANWAAPFGIALVADRLSALMLVLTATIALA
                     VLVFSARRWDRVGVSFHPLFQFLLMGLNGAFLTNDLFNLFVFFEVMLAASYGLVLHGY
                     NLRRIQAGMQYIAVNLVASLVFLIGVSLIYAASGTLNIADLGARVALLGAQDAWLLQV
                     GAAVLALAFLTKGAMWPLGFWLPTTYASASAPVGAMLVLMTKVGVYAVLRVWLAVFGE
                     GAGAAEGFGFAALTLGGMATVLFGAIGMLASQDGGRMAGYGAIVSSGTLLAVIGHSGG
                     AVLASGLYYLLGSTLAMAAFMLLIELTERIRPPGAALIAITMEAFAIEDKPEDPVGVG
                     IPGTLAFLGLSFAACALVIAGMPPLAGFVAKFSLFHAMLAAAPEGVPATTWGLIGLMI
                     LAGLAAIIALMRLGVRTFWAAGAVTPPRLQFSEAVPVGALVILCVLLTVQAGTVFDYL
                     DRTTAGLLRSGDYAARVLGEPPVLRAPGAEGAR"
     gene            15393..15911
                     /locus_tag="PKV97_03025"
     CDS             15393..15911
                     /locus_tag="PKV97_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNB04878.1"
                     /translation="MRRWLPSPLLSASLLLMWLMLNQSLEPAHWLLGGALGVLAPLLS
                     RPLQPHGHARIRRPLALFRLLWMAAIEIVRSCFNVTQIILLRRSADVNSQFIRVPLDL
                     KSPHGLALLSCLINSTPGTVWVEILPDGHELLLHVFDLHDEAWWVHTIKTRYEQPIIE
                     VFETPEPGGGRT"
     gene            15908..16192
                     /locus_tag="PKV97_03030"
     CDS             15908..16192
                     /locus_tag="PKV97_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HNB04879.1"
                     /translation="MTTATLLSAATLFALACVVLAMLLCTLRLMIGPSAQDRVLALDT
                     LWMCVMVLALLLGIRYGSQIYFEAALIIALLGFVSTIALAKFLMRGEVIE"
     gene            16272..16628
                     /gene="mnhG"
                     /locus_tag="PKV97_03035"
     CDS             16272..16628
                     /gene="mnhG"
                     /locus_tag="PKV97_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HNB04880.1"
                     /translation="MSGAESLPWWAAIPVALLLVLGGIVTLVGTLGLARLQSFYQRIH
                     GPAISIALGAGCILVASMLYFTVTQSRLVIHELLISVFVLMTAPVVSMLIMRAAVYRD
                     LRAAREMDPGNDAGNS"
BASE COUNT         2229 a         5691 c         6218 g         2726 t
ORIGIN      
        1 gaagtcgagc agccggcgcg cggtgaaggc gctggtggtg atcgcgaggc gcacggcggc
       61 gagcgcggcc cgttcgctcg ccagcaggca gcgccgcagc acgggatcga ggccgcgctc
      121 ttcggccagg gggtggtgca ccagcgcgac cagtgccagc cgtccggcgt gctcgatcgc
      181 gacctcgggc aggccgccga gcgcgagccc gtcgatgacc acgcgccgac cggcgggcag
      241 ggtggcgagg gtgtcgtgca gggcgtcgcg cgccgtgggg tcggcgtcgg ggaagcggcc
      301 gggcaggcca tggacggtga cggtccagcc gaggtggcgc agcgcctcga cgatgcgggc
      361 atcgtagaga tagccgccgg tgcgctgggc ggggtcgccg gggacgatga aatcgaggtc
      421 tgtgctcccc ctgtgagcgc tcacagcgcg ccctcgtagc tcgcccatgc gacgtgcgac
      481 tcgtgcaggc tcaccttcag ccgctccagc ccctgcgccc cggggcccag ccggccggcg
      541 cggatcgtgg cggcgaggcg gtcgaagatc accctggcca tgaactcggt ggtggtgttg
      601 cggccgcgga attcctcgag ctcgtcgagg ttgcggaaat tgaactcggc gagcaccgcc
      661 ttcagcgttg cggcggcgag gccgatgtcg accaccaggc cgtcggcgtc gagctcgctg
      721 cgctggaagc aggcgtcgac caggtaggtc gcgccatgca cccgttgcgc cgggccgaag
      781 atctcgccgc ggaagctgtg ggcgatcatg atgtgttcgc ggacggtgag gctgtacatg
      841 ggggctccgg atcgatgctc gggtggatct caggtgctcg gactggcata gcggatgcgc
      901 tcgcacagcg ccccggcggg ggcttcggcg aggcgctgca tcagcgcggg caggtcggtg
      961 aagtcggtct cgccgctgat cagcgcgtcc agacgtggat cgacgagcag cgcgagcgcg
     1021 agttccatgc ggcgacggta gtcccagcgc gggctgcgtg gcggcggcag gcggccgacc
     1081 tggctggact gcaggcgcag gcggcgggca tggaaggcgg cgccgagcgg cagctccacg
     1141 ctgcgccggc cgtaccagct catctccacg acggtggcgt cctgtccggc gagttcgagc
     1201 gcgcgcacca ggccggcggg gttgccgctg gcgtggaaga cgaggtcgca gttgccacgc
     1261 gcctgctccg ggaggcggtg aacgaggccg agcgcggcgg cgaggccggc gcggccgggg
     1321 tcggtgtcga tcagctcgag ctcgacgccg gggaggcgcg cgcacagcca ggccaccagg
     1381 ctgccgacca cgccggcgcc gaccaccgcg atgcggtcgc ccagcgcggg ggcgccgtcc
     1441 cagcaggcgt tgatcgcggt ctcgaggttg gcggccagca ccgcgcgcgc ggccggcacg
     1501 ccggcgggga gggggacgac ggcgccggcg ggtaccacat agcgctgctg atgcgggtgc
     1561 aggcagaaca ccgtgcggcc gcgcaaggcg gtggccgcgg tgccgacgcc gtcctccacc
     1621 acgccgacgc cgatgtagcc gtacttgagc ggcccgggga agtcgccctc ctggaagggg
     1681 gcgcgcatgc gttcgtattc gctttcgggc acctcgccgc ggaacaccag gctctcgctg
     1741 ccgcggctga ctgcgccgaa gaggttgcgc acccgcagct cgccgtctgc gggtgggcgc
     1801 agcggcgccg gacgcaattc gccgtagccc gggcggacgg tccagaaggc gaggttggtg
     1861 ctggacatgc cgagctcctt gtgcgaaccc tttcgcgcgg cggatgatcg aagcaggcat
     1921 ggtagcaggg cgctgcagcc actgtaggcg cgccgccagg cgcgaatggg gcctgaacga
     1981 cgtgggaaag gggcgcaagg gcagcatggc gatggagcag ggcggatctt gtgttcggcg
     2041 cggcggactt ccccgcctct ggctcgatct ggccgggggc ttcggcggcc tcgtcgcgct
     2101 cgtcgcgctg gcgtcgagca cgctcgcgct gccgctcgcg gctgcttggg cggcgggcgc
     2161 ggtgttgctc gccatcgctg ccttgatccg gcacttctgg tccgacccgc gccgcggctt
     2221 gggtccggcg aaccgggtga cgctggcgcg cgccctgctg gtcgccctcc tcgccgggat
     2281 gcttgccgcg cccgcatggg tggatgcaca tcccgtgcag gtggccgcgc tcgccgcgct
     2341 cgcgctgctg ctcgatggcg tggacggctg ggtggcgcgg cgctggcgct gcgagagcga
     2401 cttcggcgcg cgcttcgaca tggagctcga cgccttcctg atcctggtcc tgtgcgtgca
     2461 tctgctggcg atggacaagg cgggaccctg ggtgctggcg atcggtgcga tgcgctacgt
     2521 cttcgtcgcc gcgatgcggc cctggccttg gctggagcgg ccgctgcggg agagccgccg
     2581 gcgcaagctg gtctgcgtct ggcaggtcgc ctgcctgctg ctatgcctgc tgccggcgat
     2641 cggcggcgcg ctggcgacgg cgctgctggt gctggcgctg gcgctgctgg cgtggtcctt
     2701 cgcggtcgat gtgcgctggc tgtaccacac gcgtccgcaa tcctgaccgc ggaagccaca
     2761 ttcgcaagtc cctggaggag aaaggaagat ggaatcgcgc agacgttttc cccgcatgct
     2821 cgccgcggcc gtgctggcgg gcgtcctcgg tgccgcgccg gcctgggcgg agccgcgcag
     2881 ctacacgatc gacccggagc atttcgccat cggcttccag gtcgagcacc tcggctacgc
     2941 cgacatcatc ggcctgttcc tgaaggcgag cgggcgcttc gaatacgacg aggccgcgcg
     3001 cacgctgaag tcggggaggg tggtggtggc cgccgacagc gtgttcagca accacgaggc
     3061 gcgcgaccgc cacgtgcgcg acagcgactt cctcgacgcc aggaagcatc ccgagatcgt
     3121 cttcgaggcc agcggcttcg aggccggcgc cgagcgcgac gggcgcctgc acggcaagct
     3181 cgccggccgt ctgaccctgc tcgggcagac ccacccggtc gtgctcgacg tcacgctcaa
     3241 caaggccgca agctatccct tcggccaccg caagcacacg ctcggcatct cggcgcgcac
     3301 gaccatcgag cgcagccgct ggggcatgga ctacggcgtc agccgcggca tggtgggcga
     3361 tgcggtggcg ctgcgcttcg agctggaggc gatgcgcgac gattgaggcg acgatcgagg
     3421 tggcgcaggc cgcggtggcg gcggccgcga ccgcttcagc cgccgatgta ggacatctcg
     3481 atcttgcgca gcgcggcggt ctcggcggtg cggatctcgg aataacggtc ctcgcgcccc
     3541 tgccacacct gggcgatcgc cgcgtcgata cgggcatcgt cggcaccttc gcgcagcagg
     3601 gcgcgcagat cgtggccctg ctgcgcgaac aggcaggtgt agagctgtcc ctcggtggac
     3661 agacggatgc gcgtgcagct cgagcagaag gcctgggtca ccgaggagat cacgccgatc
     3721 tcgccggcgc cgtccttgta gcgccagcgc tcggcgacct cgccctcgta gttggggtcg
     3781 accggctcga gcgggaagag gcggtcgatg cgctcgacga tctcgcgcga gggcagcacc
     3841 tcgtccatct tccagccgtt ggaagcgccc acgtccatga actcgatgaa gcgcaggatg
     3901 tggccgctgt tgcgaaagtg cgcggccatc ggctcgatgt cgacgtcgtt ggtgccgcgc
     3961 ttgaccacca tgttgacctt gatcgggccc aggcccacca ccttggccgc ctcgatgccc
     4021 tcgagcacct tctccaccgg gtagtcggcg tcgttcatgc gccggaagag cgcatcgtcg
     4081 agcgcgtcca ggctcacggt gacgcggtgc aggccggcgt ccttgagccg gcgcgcgagc
     4141 ttgggcagca gcacaccgtt ggtggtgagc gtgacctcga cgccgtcgag tgcggcgagc
     4201 atgccgatca ggcggtcgac gtccttgcgc agcagcggtt cgccgccggt gatgcggatc
     4261 ttgcgcacgc cgcgcgcgac gaacagccgc gccacgcgca ggatctcctc gaagctgagc
     4321 agctgccgcc gcggcaggaa ggcgtagtcg tcgccgaaga cctcgcgcgg catgcagtac
     4381 acacagcgga agttgcagcg gtcggtgacc gagatgcgca ggtcgcgcac gctgcgcgcc
     4441 cggcggtcga ccagcgcagc gccgggcgcc gcaacgggcg cgggggccgc gggggcgagg
     4501 ggagacgcgg cctgctggat cgggatgact ttcatggtcg gctgcggctg cgggctgggg
     4561 ttcgggttgc gggattatcg agtcgtggac agcgcggcgc aaggcgaggt tcgcgcaggc
     4621 gtgcatctcg agcttcgtcg caagcccgcg gccgatcgcg actcgcgtcg ctcccacgag
     4681 aagcggagcc gccgtagccg ggggcagtgg gcgtggatca cggggcctgc ggggcggggt
     4741 gtatcattgc gctccctgcc gctccggcac cagaagaaag atcaaatcca tggccgatct
     4801 cgtgccactg ccggcgacgg ctttgcgcaa gcgctgcgac cccgcctgct tcagcttcga
     4861 caccaccgaa tcgctcgccg accacgccgg cgaccttggc caggagcgcg ccgtcgaggc
     4921 gatccgcttc ggcctcgcga tgggccacac cggctaccac gtgttcgtgc tcggggatgc
     4981 cggcagcggc aagcacgcga ccaccttccg cctgctgcgc gagcgcgcgg cgcgcgagga
     5041 cgtgccgccc gatctctgtt atctccacaa cttcgacgag ccgctcaagc cccgcctgct
     5101 gaccctgccg gccgggcgcg gcgcggcgct gcgcacgcgc atgcagacct tcatccgcga
     5161 cctcggcccg gcgctcggcg ccgcgctcga cagcgacgcc cacgtcgagc gtgtcgagtc
     5221 gctgcagagt gcgcacaagg cgcgcgagga aggggcgctg cgcgaactcg gcaaggcctg
     5281 caacgccgaa ggcatctcgc tgctgcagac ccccgagggc ttcgtgttcg cgcccaccaa
     5341 ggacggcgag ccgctgtcgc ccgaggcctt cgaggccttg ccggaggacg agcgcaaggc
     5401 catcgaggcg cgcatcggcg cgtggagcga ccgcctcgag gatctgctca acgacttccc
     5461 cggctggcgc aagtcggtgc gcgagtcgat cgaccgtgcc gagcgcgagg tgctcggccc
     5521 cgcggtcgcg cacctgctgc gcgaggtgcg cgaggcgcac gaggacctgc ccgaggtgct
     5581 cgccttcctc gacgcggtcg agcgcgacgt catcgacagc gcggtgcgcg gcaccatgct
     5641 ggaggaagaa gacgacgagg ccccagagcc cgaggagaac acgcgctacc accgctacca
     5701 ggtcaagctg ctggtggatc attccggctc gcagggtgcg ccggtggtgt tcgagaacaa
     5761 ccccggctac ggcaacctga tcggccgcat cgagcacatc gtgcagcagg gcaatcaggt
     5821 cagccacttc aacctgatcc gtgccggcgc gctgcaccgc gccaacggcg gctacctggt
     5881 gctcgacgcg gagcgcctgc tgtcgcagcc cttcgcctgg gaaggcctca agcgcagcct
     5941 gcgcgcgggc gagatccgca tcgagccgcc ggccgaggcg cagggctgga gcggggcgtt
     6001 gaccctggag ccggaggcgg tgccgagcgc gctcaaggtg gtgctgatcg gcgaccgcga
     6061 ggtctactac ctgctgatgg agcacgatcc ggagttcgcc gacctcttca aggtggcggc
     6121 cgacttcaac gacgacatcc tgcgcacgcc cgagaacgaa tgcgagtacg cccgcctgat
     6181 ggcgctgctc gcgcgcggcg cgaagctgct gccggtcgag cgcagcggtg tggcgcgcct
     6241 ggtcgaggaa gcctcgcgcc tggccgagga ctccgggcgc ctgtcgctca acacccgcgc
     6301 gctctccgac ctcatgcgcg aggccgactt ccacgcccgc cagaagggcc tcgccgccac
     6361 gacgcgcgcc gaggtcgagg aggcgctcgc cgcgcgcgcg cggcgggcca atcgttaccc
     6421 gacccgggta ctggagtcca tcctcgacgg caccacgctg atctccacca ccggcgagcg
     6481 cgccggccag atcaacggct tggtggtgat cgagctcgcc ggggaactct tcggctaccc
     6541 ggtgcggatc accgccacgg tgcgcatggg cgagggcgac gtggtcgaca tcgagcgcga
     6601 gaccgaactc ggcggtgcga tccactccaa gggcgtgctg atcctgtcgg ccttcctcgc
     6661 cgcgcgctac gcccgccacc agccgctgtc gctgtcggcg agcctggtgt tcgagcagtc
     6721 ctacgcgccg gtcgagggcg actcggcctc gctcgccgag ctgtgcgcac tgctctcggc
     6781 gctgacccag gtgccgatcc agcagcgctt cgcgatcacc ggctcggtga accagttcgg
     6841 cgaggtgcag gtgatcggcg gtgtgaacga gaagatcgaa ggcttcttcg acctgtgcag
     6901 cgcgcgcggg ctggacggca cgcagggcgt ggtgatcccg gcggcgagcg tgcgccacct
     6961 gatgctgcgc gaggacgtgg tgcaggccgc ggcggaaggc cgcttccaca tccacgcggt
     7021 gaagacggtg gatgaggcga tgaccgtgct gaccggcgtc gaggccggcg agcccgacgc
     7081 caagggcgtg attcccaagg gcacgctcaa ccacaagatc gccaccgtgc tcgccgagat
     7141 gaccgcggcg cgccacgcct attcggacgc cgacggcgcc gccggcgccc gccagcaccg
     7201 ccgccggcac ggggtgtgag ccccgcgccg gacgggccgg cttacgagaa catgtcctcg
     7261 tagatggtct gcgcccagcc gaagtcctgc gagatcgtcg ccgcggtgcg ctcgttgtag
     7321 tcgatctggg tctgctcgat gatgccctgg gcgttgacct tgtagtcgaa ctgcaccgag
     7381 atgccttcct gcggggcgat attcaccatc atgaagcaga ggttgtccgg gagccggtag
     7441 tcgggttccc gcccggcctc gcgctcggag atgtagtagg ccacgatgcg ggcgtggcgg
     7501 ttggcgacgt ggccggcctt ggggtagaag ccgaactgcg gcgacacgaa gccgaccgcg
     7561 tcgccgatga cgaagacctc cttgtccgtg cggtcgtgca ggaagagcgg atcgacgtcg
     7621 gcccagccgc cgctcgggtt gcccgcacct ggacgcggca ccagacccgc cttccaggcg
     7681 aggtcgccgg cctggtgcgg ggccatgagg atggcgtgct ggaagtcgaa atcgcccgcc
     7741 tcggtgcgga tcctgcgctt gaacggatcg acctcctcga tgcgcgcgtc ggggacgtag
     7801 gtgatgatgt ccttgtacag ctcttcgaag gtggaacgga agcccgggcc gatcgggcgc
     7861 gcgccgtcct tgtggtcgag gatgtagatg cgccccggga tcttcttctg cttgaacatc
     7921 gacgcgatca ggcaggcgcg ctcgtagggg ctgggcgggc agcgctgcgg cgggggcggc
     7981 agggtcatca cgaggtcgcc gcccttgaag gactggagcg cgttcttgag gtggaagtgc
     8041 tcggcgctcg ggatgtaggc cgccggaaag cgcgcccggg tggcctcggc catgcggcgg
     8101 tcgttgccga accaggcctc gtagttgtag cggatgccgg gggccacgat caggtagtcg
     8161 tagtggatgg cgccggccgc cgtgatcacc cgcttgccct cgcgctcgat ctcgaggatc
     8221 tcggtctgga tgaagcggta ggcgtacttc tcggcgacgg cgaggtagtc gtgggtgaga
     8281 taccgggtat ccacctgatc gaccagccac ttgttgctca tcgggcagga gaagaacacg
     8341 gggttgcgct ccagcagcac gacgtcgagc tggggggcga gctggcgcag gtacttggcg
     8401 gcggtcaccc cgccccagcc gccaccgcag atcacgacgc ggcgttgctt gccgggtggc
     8461 agcagggtgt cggtggcgcg gccgatcagg aagggcaggc tgcgctcggt gctcgcggcg
     8521 gggggcgcgg actgcgccag ggccgggccg gcgacggcgg cggtggcgag cccgccggcc
     8581 gcggtgagga agcgccggcg cgggttcgag gtaggctggt tcatgaggtc tcccgatagg
     8641 tgagccgccc cggtgcggga agccgccggg gcttgatgag ccgggcgcga cggcccggca
     8701 gggcttggcg accgcagtcg ccgcgacgga cggccgatat gaagatcggc cggatgcgtc
     8761 cgttcaggtg gagggctgcg cggcgaggcg tgcgagctgg cggtcgtgca gccgtccgaa
     8821 cacgatctgc gcgattatcg ctccgagcag cgccatcagc atgtcggcct gggcgtccca
     8881 ggggtcgccc tgggtgccga ggaattccac cgcgccgccg gcccacagct cggccgaggc
     8941 ccattcgacg agctcgtaca gcgcgctgat gccggtgcag ctcagggtga gcagggcgaa
     9001 cagccatttt cccgggcgca gcggcgaggt gcgcagcagc agctcgcggc cgaggatggc
     9061 cggggtgacg ccctggaaga agtggccgaa gcggtcgtag gggttgcgcg cgagctcgaa
     9121 ccagtcctgc accacgaagc cgaccggtac gcgcgcgtag gtgtaggcgc cgccgaggat
     9181 gaggatcagc gagaagaacg cgatcagacc gtaggtgagc ggggtgagcg ggaagcgctg
     9241 cgcgctcgcg agcaggatgg gcagcgcgat caggaccggg gccacctcgg cccaccacac
     9301 gccacggtcg tagggggcga tgcccgaggc gaggagggcg gcgaggacga ggatcagcag
     9361 ggcgagacgc agcttcatgt ggggctcccg ggggcaggtg cagggcgtgg gtttgcgcgc
     9421 cgcgtgcgga gcctgggggt ggcccggcgg gcgatcgcca gtgcgtgcaa tcctgagccc
     9481 ttgcgggctt ccccgccagc atggcccgct tgcgccggcg cagcggctgc gtacggcaag
     9541 agaaacttcg cgctcctcgg gaagtcgtac cacccccatt tgcatgcagg atctccgatg
     9601 aagcaccgtc cgctcatgct cgccgccagt gtgctggcgc tcctggcgca ggtcggcccc
     9661 gttgccgcgg ccgacgcgcc atccgcgccc gtggagccga tcgccgcgct cgacgtgccg
     9721 cgctacatgg gctcctggta cgagatcgcg cgctatccga actggttcca gaagaagtgc
     9781 gtgggggatt cgcgcgccga ctacagcctg ctggagagcg gcgaggtgcg cgtggtgaac
     9841 cgctgccgcg aggccgatgg cagcgtctcc gaggcggtcg gcgtggcgcg gcagatcggt
     9901 ggtgccgatt cgccgcggct ggaggtgcgc ttcgcgccgg cctggctgtc cttcctgccg
     9961 atcgtatggg gcaactactg ggtgctcgac ctcgaccccg actatcgcct ggtcgcggtc
    10021 ggtgagccga gccgggagta cctgtggatc ctgtcgcgca cgccgacggt ggaggcgcgt
    10081 gcctacgccg agctgctcga acgcctcgcg gcgcgcggct tcgatgtcgg gcggctcgtg
    10141 cgcggggagc aaggtggcgg cgggtgaggg cggtgcgagc gtcatcgcac ccttcagcgg
    10201 cgatcgcgac ttgcgtcgct cccacaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnntcg aaagcgctgg agcgcgccgc tcccgcgctc ccagaacccg acagacccct
    10381 gcgggagctc cgccttttca tgcgtgcccg gtaatctggc accaaatttg ctgtggtaac
    10441 ctcggcaggg tttcgccgga ccgccgcacg tcgtcctggc ccccgccccg tccctgaacg
    10501 cctttccgcc ccgatccgcc tcgatgctgc tcgcctcgct cgtcctcctc ccctttctcg
    10561 gtagcgtgct cgccgcgctg atgccgcagg acgcgcgcaa ccgcgagtcc tggctggcga
    10621 tcggggtggc ggtgggcgga ctggcgatcg ccgcggcctt gttcccgcag gtcgccgccg
    10681 gcgaggtcgt gcgtgcggag ttcgcctggc tgcccgccga gggcttggac ttcgtgctgc
    10741 gggtcgacgg cctggcctgg gtcttcagca tgctggtgct ggcgatcgga ctgctggtgg
    10801 tgctgtatgc gcgctactac atgtcgcccg aagacccggt gccgcgcttc ttcgccttcc
    10861 tgctcgcctt catgggctcg atgctggggg tcgtgctgtc gggcaacctg gtgcagctgg
    10921 tggtgttctg ggagctcacc agcctcacct ccttcctgct catcggctac tggcaccacc
    10981 gcgcggacgc tcgccgcggc gcgcgcatgg cgttcaccgt gaccgccacc ggcggcctgt
    11041 gcctgatggc cggggtgctg gtgctggggc atatcgtcgg cagctacgac ctcgacgtcg
    11101 tgctcgccgc cggcgaccag atccgcggcc acgagctcta cctccccgcg ctggtgctgg
    11161 tcggactcgg tgcactcacc aagagtgcgc agtttccctt ccacttctgg ctgccgcatg
    11221 cgatggcggc gcccacgccg gtgtcgtcct acctgcactc ggccacgatg gtgaaggccg
    11281 gggtgttcct gctcgtgcgc ctgtggccgg cgctcgccgg cacgccggaa tggacctgga
    11341 tcatcggcgg cgccggcgtg tgcaccctgc tgatcggcgc cttcgtggcc atcttccagc
    11401 acgacctcaa gggcttgctg gcctactcga cgatcagcca cctcggcctc atcacggtgc
    11461 tgatcggcat cggcacgccg ctggcgatcg tcgcggcgat cttccacatc ctcaaccatg
    11521 cgaccttcaa ggcttcgctg ttcatggccg ccgggatcat cgaccacgag accggcaccc
    11581 gcgacctgcg cgtgctgcgc ggcctgcgcc acaagctgcc gatcaccgcc acgctggcca
    11641 tcgtcgccag cgcggcgatg gcgggggtgc cgctgctcaa cggcttcctg tccaaggaga
    11701 tgttcttcgc cgagacgctg cacgtcgggg gggatcgcaa ctggtggatg tcgtacgccg
    11761 cggtggcgat gggcatgttc agcgtggcct attcgctgcg cttcatctcg gtcttcttcg
    11821 gaacgccggc gcagggcctg ccgcgcgacc cccacgagcc gccgcgctgg atgcgctttc
    11881 cggtcgagct gctggtgctg gcctgcctgg tggtgggcgt cgcccccggc gtgagcatcg
    11941 agccgctact gcacctggcg gtggtcgcca ccctgggcga ggacgcgccg gcctacgacc
    12001 tggcggtgtg gcacggcttc aacctgccgc tgctgatgag cgtgtgcgcg ctcgtgggcg
    12061 gcatgctgct ttacggggtg ctgcgccgcc atttcgacct ggcgcggcgc gagcgcgtgc
    12121 cgctgctcca ccgcctcaac ggcgcgcagg cctacgagac gacgatgctg cagctcaccc
    12181 gcggtgcgga gtggctgctg cgccgcgtcg gcacgcgccg gctgcagccg cagctgatgc
    12241 tgatgatgct cgcgctgctg ctgatcccgc tcctgctggc gggcgctccg ccggcgtgga
    12301 ccgtgatccc cgtgcggggc ttcgacccca tcttcgccgg catgtggctg atcggcgccg
    12361 gctgcgcgct ggcggcggcg tggatggcga agtaccaccg cctggccgcg ctcgcgctcg
    12421 tcggcggcac cggcatcgtc accgcggcga ccttcctgtg gctgtctgcg cccgacctgg
    12481 cgctgactca gctgatggtg gagacggtga ccacggtgct gatcctgctc ggcctgcgct
    12541 ggctgccgcc gcggatcgag tcgatcgccg gcccggtgca gccgccgtgg caggtatgga
    12601 cgcgacgcac gcgcgacctc gcgctcgccg tcggcggcgg cctcgcgctg gcggcgctga
    12661 cccatgcggt gctggtgcat ccggcctcgg acacgatcgc ggacttcttc ctgctcaacg
    12721 ccctcggcgg cggcgggggc agcaacgtgg tgaacgtgct gctggtggat ttccgcagct
    12781 tcgacaccct gggcgagatc accgtgctcg ccgtcgtcgc gctcaccgtg tatgcgttgc
    12841 tgcgccgctt ccgcccggcg ccggaaagcg ccggcatccc gccgcagcag cgcttcgagg
    12901 gcgatccggc gagccggcag agccccgccg agcaggccgg cagcggctac ctgctggtgc
    12961 cggggatcta cctgcgcctg ctgctgccct tcatgggggt gatcgcggtg tatttcttca
    13021 tgcgcgggca caacctgccg ggcggcggct tcgttgccgg actgatcttc gcggtggcga
    13081 tcctggtgca gtacatgctc gccggcacgg tgtgggtgga gagccgcctg aacctgcgcc
    13141 cgcatcgctg gctgggcttc ggactgctgc tggcctgcac caccggcctg ggggcgtggt
    13201 ggttcgggca tcccttcctc accacccaca ccgcgcacgt ggagtggccg ctggtgggcg
    13261 agctgcacct gccgagcgcc ttcgtgttcg acctcggcgt gttcgcggtg gtggtcggca
    13321 ccacgatgct gatgctggtc gcgctcgcgc accagtcgct gcgcgcgcac cgccacccgg
    13381 gcgaggacct gacggcggac ggacagggcg gtgccgaggg gaggcggatc tgatggaact
    13441 ggtactggcg atttccgtag gcgtggtgtt cggcgcgggg atctggctga tcctgcgtcc
    13501 gcgcaccttc cagctcatca ccgggctgct gttgatgtcg tacgcggtga acctgttcat
    13561 cttcgcgatg gggcggctgt gggtggacag gccgccgatc acgcccacgc ccgccatcga
    13621 tccggcgcag ttcgccgacc cgctgccgca gggcctcgtg ctcaccgcga tcgtgatcgg
    13681 cttcgccacc accgcgctct acctggtgat gatgatcggc gcgcgcgggc tgtcgggcag
    13741 cgaccacgtc gatggcgagg agccgcgcga atgacggtgt tgccctggct caaccacctg
    13801 atcatcgtgc ccgtgctgct gccgctgatc gtcggcgcgc tgatgatccc gatcaaccag
    13861 aagcgccacg ggctcaagtt cggcctcggc ctcgcgagcg gcatgctgct gtgggcggtg
    13921 gcggtcgtgc tgctggtgct ggccgacggc gggtactggc ccggcggcat cggcgtgtat
    13981 ctggcggcca actgggctgc gcccttcggc atcgcgctgg tggccgaccg cctctccgcg
    14041 ctgatgctgg tgctgacggc gacgatcgcg ctcgccgtgc tggtgttctc ggcgcggcgc
    14101 tgggaccgcg tcggcgtgag cttccacccg ctcttccagt tcctgctgat ggggctcaac
    14161 ggcgccttcc tgaccaacga cctgttcaac ctcttcgtgt tcttcgaggt catgctggcg
    14221 gcgtcctacg ggctcgtgct gcacggctac aacctgcgcc gcatccaggc cggcatgcag
    14281 tacatcgcgg tgaacctggt ggcttcgctg gtgttcctga tcggggtgtc gctgatctac
    14341 gccgcctccg gaacgctcaa catcgccgac ctcggcgccc gcgtggccct gctcggcgcg
    14401 caggacgcct ggttgctgca ggtcggggcg gcggtgctgg cgctcgcctt tctgaccaag
    14461 ggggcgatgt ggccgctcgg gttctggttg cccaccacct acgcctcggc ctcggcgccg
    14521 gtgggggcga tgctggtgct gatgaccaag gtgggcgtgt atgccgtgct gcgggtgtgg
    14581 ctggcggtgt tcggcgaggg ggcgggcgcc gcggagggct tcggcttcgc cgcgctgacc
    14641 ctcggcggca tggcgaccgt gctgttcggc gccatcggca tgctcgccag ccaggacggc
    14701 gggcgcatgg cgggctatgg cgcgatcgtg tcctcgggca cgctgctggc ggtgatcggg
    14761 cattccgggg gagcggtgct ggcctcgggc ctctactacc tgctcggctc cacgctggcg
    14821 atggcggcct tcatgctgct gatcgagctc accgagcgca tccgtccgcc gggcgcggcg
    14881 ctgatcgcga tcaccatgga ggcctttgcg atcgaggaca agccggagga tccggtcggc
    14941 gtcggcattc ccggcacgct cgccttcctc ggcctgagct tcgccgcgtg cgcgctggtg
    15001 atcgccggga tgccgccgct ggcgggcttc gtcgccaagt tcagcctctt ccacgccatg
    15061 ctcgcggccg cgcccgaagg cgtcccggcg acgacctggg gcctgatcgg gctgatgatc
    15121 ctcgccgggc tggcggcgat catcgcgctg atgcgcctgg gggtgcgcac cttctgggct
    15181 gcgggcgcgg tgacgccgcc gcggctgcag ttctccgagg cggtgccggt gggggcgctg
    15241 gtgatcctgt gcgtgctgct gacggtgcag gcgggcacgg tgttcgacta cctcgaccgc
    15301 accaccgcgg gcctgctgcg cagcggcgac tacgcggcgc gcgtgctcgg cgagcctccg
    15361 gtgctgcgcg cgccgggcgc ggagggtgcg cgatgagacg ctggctgcct tcgccgctgc
    15421 tgtcggcgag cctgctgctg atgtggctga tgctcaacca gagcctggag cccgcgcact
    15481 ggctgctcgg cggcgcgctc ggcgtgctcg cgccgttgct gtcgcggccg ctgcagccgc
    15541 acggccacgc ccgcatccgg cgcccgctgg cgcttttccg gctgttgtgg atggcggcga
    15601 tcgagatcgt ccgctcctgc ttcaacgtca cccagatcat cctgctgcgg cgctcggcgg
    15661 acgtgaactc gcagttcatc cgcgtgccgc tcgacctgaa gagtccgcac gggctggccc
    15721 tgctgtcctg cctgatcaac tcgacgccgg gcacggtgtg ggtggagatc cttcccgacg
    15781 gccacgagct gctgctccac gtcttcgatc tgcacgacga ggcgtggtgg gtgcacacca
    15841 tcaagacgcg ttacgaacag ccgatcatcg aggtgttcga gacgcccgaa cccggaggag
    15901 gccggacatg acgaccgcga cgctgctctc ggcggcgacc cttttcgcgc tggcctgcgt
    15961 ggtgctggcc atgctgttgt gcacgctgcg gctgatgatc ggcccctcgg cacaggaccg
    16021 cgtgctggcg ctcgacacgc tgtggatgtg cgtgatggtg ctggcgctgc tgctgggcat
    16081 ccgctacggc agccagatct atttcgaggc ggcgctgatc atcgcgctgc tcggcttcgt
    16141 gtccaccatc gcgctcgcca agttcctgat gcgcggggag gtgatcgaat gagcgcgtcc
    16201 gcacgaccgc tcgaaggaaa gcgctcccct ccctcgggga ggaggtcgcg cagcgacagg
    16261 agggcagccc aatgagtggt gccgaatccc tgccgtggtg ggcggcgatc ccggtggcct
    16321 tgctgctggt gctgggcggc atcgtcaccc tcgtcggcac gctcggcctg gcgcgcctgc
    16381 agagcttcta ccagcgcatc cacggccccg cgatcagcat cgcgctcggc gccggctgca
    16441 tcctggtggc atcgatgctg tacttcaccg tgacacagtc gcgcctggtg atccacgagc
    16501 tgctgatcag cgtcttcgtg ctgatgaccg cgccggtggt gtcgatgctg atcatgcgtg
    16561 ccgcggtgta ccgcgacctg cgtgccgcgc gcgagatgga cccggggaac gatgcgggca
    16621 attcgtgagg gctcgcggca cggcggcgaa cccggtttca gtgctcgttg acgcatcgtg
    16681 gcgcggggcc ggacgccggt gctcgttcgc gcgccgtatc gcgacttgcg tcgctcccac
    16741 cgcgggctcg gtcgtcatcg cttccgtgag ccgtcggggg actgcccgcc tgtaggagcg
    16801 acgcaagtcg cgataggcga ggtcgatccg gcgcggaata gtgccgcgtc gcgctggcat
    16861 cgccaacaaa aaagccaacc ccgaaggatt ggctttcttg ttggcgatgc tggctcctcg
    16921 acctgggctc gaaccaggga cctacggatt aacagtccgg cgct
//
LOCUS       DAOQNN010000025        16429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1368_length_16429_cov_8.272322, whole genome shotgun sequence.
ACCESSION   DAOQNN010000025 DAOQNN010000000
VERSION     DAOQNN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 16429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16429
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1368_length_16429_cov_8.272322"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..175
                     /locus_tag="PKV97_03040"
     CDS             8..175
                     /locus_tag="PKV97_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HNB04881.1"
                     /translation="MQRLPPAVRARHTHFSACAGCGRVYWEGSHWQRLRARIDALLAQ
                     ARAAPQDEAAD"
     gene            complement(181..657)
                     /locus_tag="PKV97_03045"
     CDS             complement(181..657)
                     /locus_tag="PKV97_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04882.1"
                     /translation="MRAVYLSGLLVVVLAVVTFWMTAGLQPGVTVLQFAWTPRGFGEI
                     VHVWSPGDLARYRLHLPVDSLLLLAYGSFGWLLATRTALYASLPGRARVAARFVLPLA
                     AVFDAVENALHAWLTEMPRFGMHGLYLLSTTCSALKWALLFGFAALVLWALAREAD"
     gene            838..2040
                     /locus_tag="PKV97_03050"
     CDS             838..2040
                     /locus_tag="PKV97_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB04883.1"
                     /translation="MTGPHLLPLLLLYALMLPVTGMVPVLPEFTAQRFPGLGQFASHF
                     FMSINMIGALLGAPIAGLLSDRLGKRRLLAVGALAVNGIALLGIAWAWRSTESYALLL
                     ALRLVEGFAHMSALSLLMALAADHAGKAGLGARMGAVGASISLGVATGAPLGGIIGDI
                     DPFWVPLGGGLLSLAMAALGFVRLAEGGGTRPRMAASEIVDTLRNRRQLLIPLAFSFA
                     DRLTVGFIVSTLSLYLGLVIGFDARQIGIAMAAFLIPFSVLTWPAGHLSRHWDPLWMM
                     VIGSVLYGVFLAVLGFVPGDRVVATMAAGGVIAALMYAPSLVLAAQYGGSDCRASALA
                     AFNMAGSLGFAAGPLLSSALLAFFGLVLERPYPPVFVAIGLIEVVLALAVLLLVRRGR
                     LQAGAATA"
     gene            complement(2051..2494)
                     /locus_tag="PKV97_03055"
     CDS             complement(2051..2494)
                     /locus_tag="PKV97_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNB04884.1"
                     /translation="MALLNWKADYCTGNAKIDEDHRTLFERVNDFHFAWQQNRDRKDI
                     ARVLNRLVEYAEEHFAREEEIMRASGYPGLARHQAIHEALFEQIFVLQGHFEDGRIRI
                     DRDTVDFLRGWLVDHIVGEDMKFAAYVESGDEPAPARQASDIDVV"
     gene            complement(2651..4024)
                     /locus_tag="PKV97_03060"
     CDS             complement(2651..4024)
                     /locus_tag="PKV97_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNB04885.1"
                     /translation="MTGKGAGNAQSGWSARLAARPLFMCGFRPFFLLTALHAVLVLLA
                     WGGFLGFGLALPAVAGGPFVWHAHELMFGFGLAAVAGFVLTAVPEFTATPAFAPRVVL
                     RLVLLWLAARAAFWLSGGLAAVAESWLGGEEGQAVARWIGHLPAALLEIGFACALLAA
                     VAPRLWRDPERRHLGFQWGLAAMAMAVAGFHLAVLRGEYPMRWVLVGLGVMMTLVVVA
                     MSRISMRVMNDALDAARARRLPAHTLRRDEEAELELPSYRARPPRRNLAIAAIAVHTV
                     AEYLLPGSPITGWLGLGAAAAVFNLLNDWHVGRALFTRWAFLLYAVYWLLALGYLLLG
                     LARLGAPLAPSAGTHLLAIGAMGLSILAVLCIAGRTHAGYPLDERPWVGAAAVSIVLA
                     ALLRAAAGLPDMPASALQLLATLAWITAFALAAWRLGVLFVAARVDGGAGCEEYHAPR
                     AAEAVPP"
     gene            complement(4063..4977)
                     /locus_tag="PKV97_03065"
     CDS             complement(4063..4977)
                     /locus_tag="PKV97_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB04886.1"
                     /translation="MNTISTAELRVHGLSHAFARDEVLSGIDFRVRAGEVVALVGPSG
                     CGKTTLLHLAAGLLTVQQGRVDSGFASTAFMFQQPRLLPWKSALDNVALGLKAAGVKR
                     AERHFRARALALRLGLAHRDLDKFPHQLSGGMQSRVALARALVLAPELLLLDEPFSAL
                     DVGLKEELYRLLLDHQAERGMGVLMITHDLMEAVRLSDAILVMAPAPGRIVCRFELDR
                     PAARRDDAWVYRHTAELLQVPDVRASFGLPPAVAVARDRAGSAPRPEFVRQGGLAAAA
                     RAEPLSGLRLVSPTSRPNPVQGGKRCGA"
     gene            complement(4974..5765)
                     /locus_tag="PKV97_03070"
     CDS             complement(4974..5765)
                     /locus_tag="PKV97_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB04887.1"
                     /translation="MNLLRAWLAGLPAYLWSGWGAIASLCLLLAAWEAVSLAYGPLVM
                     PDPRAAFATLWCLIDSGAAWPELAATARRALTGYALSVVAGSVLGLVAGLSMTASMMA
                     RPLVTVLMGTPPIAWLVLAMLWFGASDGTPVFTVFVASFPVIFIGALQGTRTLDNQLH
                     GMAAAFRLPRRMMLTDVYLPHVVSYLFPAWIAALGTAWKVVVMAELLATQDGVGAALA
                     VSRSHLDTAATMAWIAAVVGLLLAVEYLVLEPIKREVERWREQGA"
     gene            complement(5762..6808)
                     /locus_tag="PKV97_03075"
     CDS             complement(5762..6808)
                     /locus_tag="PKV97_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNB04888.1"
                     /translation="MGQDRRRPMAPPAWMRAVMHVCVVCAFLLVTGIVHAQEQKLAKL
                     TLAGPSAAVSNALIHMVDSGALADLAEVVEFVPWRDPDQLRVMALDGRADVLAMPTNV
                     AANLYNRGAKLQLINVSTWGVLWLVSRDPALKSLADLRGKELAMPFRGDMPDIVLQLL
                     AEGQGMDVRKDLNLRYVASPVDAMQLLVMRRVDHALLAEPAASMALRKTGSFPLSVIA
                     PELHRSVDLQKEWGRVFARAARIPQAGIAVMGALRERPELVARIEAEYARALAWCKLN
                     AAACGKAVAARIDVLSAEAVADSIAVSQLEVVPSRDARAELEHLFAKLVARNPALVGG
                     KLPDDGFYGGAKAP"
     gene            complement(6810..8861)
                     /locus_tag="PKV97_03080"
     CDS             complement(6810..8861)
                     /locus_tag="PKV97_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB04889.1"
                     /translation="MLRLRPLAVAVLGLSAFPVSSLVHAETLLGAQTVTAKGYAADAL
                     ETPQAVEILQAPAAGGTVAGALLRGKPGLAAQSDGAWGQNPVLRGLKKESVVVMVDGV
                     RMNSAQPQGAIASFLDLGLLERAEVVKGPGSVLYGSGAMGGVVNLITPDVGFSAEEAV
                     GGRVGARFSSVDRGVAGAALLTRSSAHGALMLGVAGRDVDDYRSPDGREDRTGYQSDT
                     LLAKYKHKLSEDLTLRVNLQRHEDEDVWYPGSARTGAGIPKPLGTATIHSPEQRRELY
                     ELGVDAKLGEGTLSAELYRQEVFRQIRAFSSAKQRDYVRNDVRFVTDGAKAKYLFPLG
                     ADHLLTVGADLWRMRGDPERYIDNNPPAFDNNLRNDPFDKGEIESAGVFVQDEFSLGD
                     TRIVAGARFDRVTGDAEGKGMTQTTGLENADNNLSWSLGAIQPLSERLSAYANLGRAY
                     RAADMRERFEDSARGDGYFHVGNPQLDPEVSTSLELGLKRGGVGLRYQLAAFYTRIDD
                     YIAGRVTGAVHPQNGLPFKLTENLDKVTIYGIEGSASMPVGAFVADAGFTWLRGENHQ
                     DDEPLFQMPAPELTLGLGQAAERGFWWRAQLRAVAEQDRIATRFSNGTENPTAGFVTA
                     DAEFGWRFGKLGAFESAGLAVQLSNLADKRYHEHQTEGVSGNELAAPGRGVALSLNGS
                     F"
     gene            9064..9273
                     /locus_tag="PKV97_03085"
     CDS             9064..9273
                     /locus_tag="PKV97_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04890.1"
                     /translation="MKLSRLYRPQDRRFWLMIVLNLLSAILAWLLRSWPLVPLATVVV
                     ALFALGNAALGMFIAWDLLRDERED"
     gene            complement(9426..10280)
                     /locus_tag="PKV97_03090"
     CDS             complement(9426..10280)
                     /locus_tag="PKV97_03090"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNB04891.1"
                     /translation="MSSVRAIFLSDVHLGTRACQAERLLDFLREHESDYLYLLGDIVD
                     FWAMKRSVQWAPSHNTVVQKVLRRARHGCQVIFIPGNHDEALREHAGVAFGDIRVESE
                     WIHHTADGRRYWLVHGDEYDQVTRHHRWVALLGDVAYNALVRINLLLSRARRLLGVPG
                     YWSLAGYAKQKVKKAVSFIFDFEDAVAHAAQQRGVDGVICGHIHWASDRRIGDIRYLN
                     CGDWVDSCSAVVEHHDGRIEVLQWGALGAHALAPLPALPAPRPASVRPQSAPVPAGAE
                     RIYLMPDI"
     gene            complement(10284..11510)
                     /locus_tag="PKV97_03095"
     CDS             complement(10284..11510)
                     /locus_tag="PKV97_03095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNB04892.1"
                     /translation="MRTLDYTTEDLCRAPRLRIALVTETWAPEVNGVAMTLGRMVDGL
                     IRRGHGVQLIRPRQRPGETAAHGEGLEEVLARGLRLPRYDGLKLGLPARVRLVREWSR
                     QRPDLVHVATEGPLGWTAVTAANKLRIPVSSDFHTNFDHYSGHYGMGWLRQPVAAYLR
                     RFHNRSAVTFVPTAALAAQLSAQGYRSVEVISRGVDTALYSPARRDEALRRAWGLPPG
                     GLAVISVGRLAPEKNLGLAMRAFAAIRRLRPDARMVLVGDGPQRAALARAHPDAVFVG
                     MRHGEDLAAHYASADLFLFPSLTETFGNVTLEAMASGVCPVAYDYAAAAEVIRDLGNG
                     ASVACGDEEGFIARAVQMAGADALRAELARAARRSAEAIDWERVNDRFAAALLRVWRA
                     GSGRPLDLPEPRPEET"
     gene            complement(11600..13249)
                     /locus_tag="PKV97_03100"
     CDS             complement(11600..13249)
                     /locus_tag="PKV97_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNB04893.1"
                     /translation="MQSKPGLGKCFVTAAALAALALMASGVHATAAGEAAVVAVEPAT
                     SDSALALRIEQRLRERAATADDPIALFYLARAYRSVWTEPARVHALLAAVEAVRGHGL
                     DAADFAPARLRAGAVPAADPERAAERELLLTDTLAALLFQLRHGKVDPRALYREWNFT
                     PPPSPYERAAELARVLQAPDLAAAVDAYAPDLPLYRALRAELLAQQGRLAVGDWPKVA
                     AGPTLKPGASSSRVASLRARLAAAGERVSEARDKSHYDEALVEAVKRFQAAHGLQADG
                     VLGAQTLEALKASPAQRVAQIRANLERLRWVASDLQGDRLLVDIVGYHADLVLDGQPV
                     WSSRVIVGKPKRRTPSLLDSVTHLVLNPKWVVPPTILREDVIPGAARNPSYLANRRLR
                     VVDRSGQTVDPATIDWSGARQSGFPYRVEQQSGADGSLGRIKFSLSNPYVIYLHDTNA
                     RSLFKRAERALSSGCVRVEKPEELAVLLLADSGRWSAQALQAALDSGRTRTVDVGRDV
                     KVLLHYATAALDEAGRVLLRNDIYGYDAAIVAALDAPAPAR"
     gene            13293..13856
                     /locus_tag="PKV97_03105"
     CDS             13293..13856
                     /locus_tag="PKV97_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNB04894.1"
                     /translation="MSKIVRNQVVPHRRLLLKGLATLPLGLSAAAQAAQRDPQLDFRH
                     THTDERLSVAFRNRQGYIQPALQRIDWLLRDFRTGESTRMDPRLYDMLHALSLACGGN
                     TFEIISGYRSPTTNTMLRKTRGGEVARRSLHMEGKAIDIRLVGVDTARLRDAALALGG
                     GGVGYYPDSDFVHIDTGPVRSWGPRSA"
     gene            complement(13887..14252)
                     /locus_tag="PKV97_03110"
     CDS             complement(13887..14252)
                     /locus_tag="PKV97_03110"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNB04895.1"
                     /translation="MESPFKGKTGLRRIFNAARYSMDGLRAAYRHEDAFRQEIWLTLV
                     AVPLALWLGEGAVERALMIGSVLLVLVVELLNSAVEATVDRVSLERHELAKRAKDVGS
                     AAVLLALLHAAVVWGLLLF"
     gene            14376..>16429
                     /locus_tag="PKV97_03115"
     CDS             14376..>16429
                     /locus_tag="PKV97_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB04896.1"
                     /translation="MTRTIGRFEIRRELGRGAQSVVHLAWDPQLEREVALKALHLGPR
                     PGHDNASLLAEARAVSRLRHPGIVPVFEAGEDGGDPYLVFEYVPGESLAALLDRRGPL
                     PAGEAVERMLEILAALAEAHAQGVIHRDLKPSNVLLDGGGHARVMDFGIARRIEAAGA
                     SEGLSGTPGYMAPEYIERREIGPRNDVFAAGVLLIELLAGRPLAREGGVKRVLERTRS
                     TPVTLPADCPPVDEALAAIALRAAALDPLARFADAAEFRLALEAWRQPAETDPEAGSE
                     ARPGAVDFLMRRMKHRGDFPALSESVVAINRIAASESESVGTLSALILRDFGLTNKLL
                     RVVNSAHFRPAGGGRISTISRAVVVLGFDAVRNIAITVLLFEHLQNKSNASQLQEEFL
                     RACLAGLFARELARRLRLRDGEQSYICAVFHNLGRLLCQFYFPEESEDIRRVVQQHGC
                     SEESAAQRVLGAGYEELGIALARAWGFPELIQNSMRTLPEGPVHAPRSADERLQSLAA
                     CANACCDAVSRLAPAERERALADIAARFEEAVPVPAREAREHLRSAVDEIGEFARVVK
                     VSLAQTRFGRNLRHFIDGSTPAEGARVDATLMPASALLEQSLPAEGGAEGQRAADPQA
                     VLSAGIQDISNSLVEDFRLNDVLRIILETMYRAMGFKRVLLCVRDARSNAMCGRFGFG
                     PEV"
BASE COUNT         2457 a         5977 c         5718 g         2277 t
ORIGIN      
        1 ggcggtggtg cagcgcctgc cgcccgcggt gcgcgcgcgt cacacacact tttccgcctg
       61 cgccggctgc ggccgggtgt attgggaagg ctcgcactgg cagcgcctgc gcgcgcgcat
      121 cgacgccctg ctcgcgcagg cgcgcgcggc gccgcaggac gaagccgcgg actgagcgcc
      181 tcagtctgct tcgcgcgcca gcgcccacaa caccagcgcg gcgaagccga acagcagcgc
      241 ccacttgagc gccgagcagg tggtcgacag caggtacagc ccatgcatgc cgaagcgcgg
      301 catctcggtc agccaagcgt ggagcgcatt ctccaccgcg tcgaacacgg ccgcgagcgg
      361 caggacgaag cgtgccgcaa cgcgtgcccg cccgggcagc gacgcgtaca gcgccgtgcg
      421 ggtcgcgagc agccagccga agctgccgta ggccagcagc agcaggctgt cgaccggaag
      481 gtggaggcgg tagcgggcaa gatccccggg cgaccacaca tggacgatct cgccgaagcc
      541 gcgcggcgtc cacgcgaact gcagcacggt cacgcccggc tgcagccccg cggtcatcca
      601 gaaggtgacg acggcgagca caaccaccag cagtcccgac agatacacgg cgcgcatgcg
      661 cgaacctccg aagtgagacg cacacggggt gccgacacga acccggccgc ggcggcggcg
      721 gcgggtcgcc aggattgtag ccgggccttg atcggcaaca gacgacggtc gcggaggtcg
      781 aagtatcatg cggcgcaccc gcctccgaac ccagcgcgcc gacaccgcgc cgacaccatg
      841 accggacctc atctcctgcc gctgctgctg ctctacgccc tgatgctgcc ggtcaccggc
      901 atggtgccgg tgctgcccga gttcaccgcg cagcgctttc ccggtctggg ccagttcgcc
      961 agccacttct tcatgtcgat caacatgatc ggcgcgctgc tcggcgcgcc gatcgccggc
     1021 ctgctctccg accgcctcgg caagcgccgc ctgctcgcgg taggcgcgct cgccgtgaac
     1081 ggcatcgcgc tgctgggcat cgcctgggcc tggcgcagca cggagagcta cgcgctgctc
     1141 ctcgcgctgc gcctcgtcga gggcttcgcc cacatgtcgg cgctgtcgct gctgatggcg
     1201 ctcgccgccg accacgccgg caaggccggg ctgggcgcgc gcatgggcgc ggtgggcgcc
     1261 tcgatcagcc tgggtgtggc caccggcgcg ccgctcgggg gcatcatcgg cgacatcgac
     1321 cccttctggg tgcccctcgg cggcggcctg ctctcgctgg cgatggccgc gctcggcttc
     1381 gtccgcctcg ccgagggcgg cggcacgcgg ccgcgcatgg ccgcatccga gatcgtcgac
     1441 accctgcgca accgtcgcca gttgctgatc ccgctcgcgt tctccttcgc cgaccgcctc
     1501 accgtaggct tcatcgtgtc gacgctgtcg ctctacctcg gcctggtgat cggtttcgat
     1561 gcgcgccaga tcggcatcgc gatggcggcc ttcctgatcc ccttctcggt gctcacctgg
     1621 cccgccggcc acctgtcgcg gcattgggat ccgttgtgga tgatggtgat cggcagcgtg
     1681 ctctacggcg tcttcctcgc cgtgctcggc ttcgtcccgg gcgatcgggt ggtggcgacg
     1741 atggccgcgg gcggcgtgat cgcggcgctg atgtatgcgc cctcgctggt gctggccgcg
     1801 caatatggcg gcagcgactg ccgcgccagc gcgctggccg ccttcaacat ggcgggctcg
     1861 ctcggctttg ccgccggccc gctgctcagc agcgcgctgc tcgccttctt cggcctggtg
     1921 ctggaacgcc cctacccgcc ggtcttcgtg gcgatcgggc tgatcgaggt cgtgctcgcc
     1981 ctcgcggtgc tgctgctggt gcgccgcggc cggctgcagg ccggcgccgc gacggcctga
     2041 gggcccgggt tcagacgacg tcgatgtcgc tcgcctgccg ggccggcgca ggttcgtcgc
     2101 cgctctcgac ataggccgca aacttcatgt cctcgccgac gatgtgatcc accagccagc
     2161 cgcgcaggaa gtcgaccgtg tcgcgatcga tgcggatgcg cccgtcctcg aagtggccct
     2221 gcagcacgaa gatctgctcg aacagtgcct cgtggatcgc ctggtggcgc gccagtcccg
     2281 ggtagccgct ggcgcgcatg atctcttcct cgcgcgcgaa gtgttcctcg gcgtactcga
     2341 ccaggcggtt gagcacgcgc gcgatgtcct tgcggtcgcg gttctgttgc cacgcaaagt
     2401 gaaaatcgtt gacgcgctcg aacaaggtgc ggtgatcctc gtcgatcttc gcgttaccgg
     2461 tgcaatagtc ggctttccag ttgagcagcg ccatcgtcgg gtctccaggc tcggacggcg
     2521 attccgctgc ggagccatcc acggccacca gcttagcgcg ttcggtgcca gcactgccac
     2581 gaacccggtc acgctggcct gcgtcggtcc gacccgagcg cggcgccatc accgcaggcc
     2641 cgtgcccgca tcagggcggg acggcctcgg ctgcccgggg agcgtggtat tcctcgcacc
     2701 cggcaccgcc atccacccgc gcggccacga acaacacgcc caaccgccac gcggccagcg
     2761 cgaaggctgt gatccaggcc agcgtggcaa gcaactgcaa ggcggatgcg ggcatgtcgg
     2821 gcaggccggc ggccgcgcgc agcaaggccg cgagcacgat cgagactgcc gccgccccca
     2881 cccacggacg ctcgtcgagc gggtagccgg cgtgcgtgcg cccggcgatg cagagcaccg
     2941 ccaggatgga caggcccatc gcgccgatcg ccagcaggtg agtcccggcg ctcggtgcca
     3001 gcggcgcccc cagacgggcg aggccgagca gcaggtagcc gagcgccagc agccagtaca
     3061 ccgcatagag caggaaggcc cagcgcgtga acaaggcgcg cccgacgtgc cagtcgttga
     3121 gcaggttgaa caccgccgcc gccgcgccca gccccagcca accggtgatc ggcgagcccg
     3181 gcaggaggta ttcggccacc gtatggaccg cgatcgccgc gatcgccagg ttgcgccgcg
     3241 gcggccgcgc gcggtaggaa ggcagttcca gctcggcctc ctcgtcccgg cgcaacgtat
     3301 gcgcgggcag gcggcgcgcg cgcgcggcgt cgagcgcgtc gttcatgacg cgcatcgaga
     3361 tccggctcat cgccaccacc accagcgtca tcatcacgcc cagccccacc agcacccagc
     3421 gcatcgggta ctcgccgcgc agcacagcga ggtggaagcc cgccaccgcc atcgccatcg
     3481 ccgccaggcc ccactgaaag ccgaggtggc gacgctcggg gtcgcgccac agccgcggcg
     3541 ccaccgcggc cagcaatgcg caggcgaagc cgatctcgag cagcgccgcg ggcaggtgcc
     3601 cgatccatcg cgcaacggcc tgcccctcct ccccgccgag ccagctctcc gcgacggcgg
     3661 cgagcccgcc cgacagccag aaggccgcgc gcgccgccag ccacagcagt accagacgca
     3721 gcacaacccg cggcgcgaac gccggcgtgg cggtgaactc cggtaccgcg gtgagcacga
     3781 agcccgccac cgcggcgagc ccgaagccga acatcagctc gtgcgcgtgc cacacgaagg
     3841 ggccgcccgc gaccgccggc agcgccaggc cgaagccgag gaagccgccc caggcgagca
     3901 gcaccagcac cgcatggagc gcggtgagga ggaagaaggg ccggaagccg cacatgaaca
     3961 gtggccgtgc cgcaagccgc gcgctccagc ccgattgcgc attgcccgca cccttcccgg
     4021 tcacggcccc gcgtccagca ttcatcgtcc tgtcacccat gctcatgccc cgcaacgctt
     4081 gccgccctgc accgggttcg gtctggaagt cgggctcacc aggcgcagcc cggacagcgg
     4141 ctcagcgcgt gcggcagcgg ccagcccgcc ttgccgcacg aactctggcc gcggcgcaga
     4201 tcctgctcga tccctcgcca ccgccaccgc tggtggcaaa ccgaagctcg cgcgtacgtc
     4261 cggcacctgc agcagctctg cggtgtgccg gtacacccag gcgtcatcgc gccgggccgc
     4321 cgggcggtcg agctcgaagc ggcacacgat gcgcccgggc gcgggcgcca tcaccaggat
     4381 cgcatccgac agccgcaccg cctccatgag gtcgtgggtg atcatcagca cgcccatgcc
     4441 gcgctcggcc tggtggtcga gcagcaggcg ataaagctct tccttgaggc cgacgtccag
     4501 tgccgagaac ggctcgtcga gcagcagcag ctcgggcgcg agcaccagcg cgcgcgccag
     4561 cgccacccgg ctctgcatgc cgcccgagag ctggtggggg aacttgtcga ggtcgcgatg
     4621 ggcgagtccg aggcgcaagg ccagcgcgcg cgcgcggaag tggcgctcgg cgcgcttgac
     4681 gccggccgcc ttgaggccga gcgcgacgtt gtcgagcgcg ctcttccagg gcagcaggcg
     4741 cggctgctgg aacatgaagg cggtggaggc gaagccgctg tccacccggc cttgctgcac
     4801 cgtcagcagg ccggccgcca ggtgcagcag cgtggtcttg ccgcagcccg agggtccgac
     4861 cagggccacg acctcgcccg cacgcacgcg gaagtcgatg ccagagagca cctcgtcgcg
     4921 cgcgaaggca tgggagagcc cgtgcacgcg cagctcggca gtcgaaatcg tgttcatgcg
     4981 ccctgctccc gccagcgttc gacctcacgc ttgatcggct ccagcaccag gtactccacc
     5041 gccagcagca ggccgaccac cgcggcgatc caggccatgg tcgcggcggt gtccaggtgc
     5101 gagcgcgaca ccgcgagcgc cgcgcccacg ccgtcctggg tggcgagcag ctcggccatc
     5161 accaccacct tccaggccgt gccgagcgcg gcgatccagg ccgggaacag gtaggacacc
     5221 acgtgcggca ggtacacgtc ggtgagcatc atgcggcgcg gcaggcggaa ggccgcggcc
     5281 atgccgtgga gctggttgtc gagcgtgcgc gtaccctgca gtgcaccgat gaagatcacc
     5341 gggaaggacg ccacgaacac ggtgaacacg ggtgttccat cactcgcgcc gaaccacagc
     5401 atcgccagca ccagccaggc gatcggcggc gtgcccatga gcacggtgac gagcggacgc
     5461 gccatcatcg aggcggtcat cgacagcccg gcgaccaggc cgagcacgct gccggcgacc
     5521 accgacagcg cgtagccggt aagcgcgcgg cgtgcggtgg cggccagttc cggccacgcc
     5581 gcgccactgt cgatcaggca ccacagcgtg gcgaaggccg cgcgcgggtc gggcatgacg
     5641 agcgggccgt aggcgagact caccgcctcc caggcggcga gaagcagaca gaggctggcg
     5701 atcgcgcccc agccactcca caggtaggcc ggcaagccgg ccagccaggc gcgcagcagg
     5761 ttcatggcgc cttcgctccg ccgtagaagc catcgtccgg cagcttgccg ccgaccagtg
     5821 cgggattcct cgccaccagc ttggcgaaca ggtgctcgag ctcggcacgc gcatcacggc
     5881 tcggcaccac ctcgagctgg ctcaccgcga tcgaatcggc caccgcctcg gcgctgagca
     5941 cgtcgatgcg cgccgccacc gccttgccgc aggccgcggc gttgagcttg caccacgcga
     6001 gcgcgcgcgc gtactcggcc tcgatgcgcg ccaccagctc ggggcgctcg cgcagcgcgc
     6061 ccatgaccgc gatgcccgcc tgcgggatgc gtgccgcacg cgcgaacacc cggccccact
     6121 ccttctgcag gtccacgctg cggtgcagct cgggcgcgat cacgctgagc gggaaggacc
     6181 cggtcttgcg cagcgccatc gaggccgccg gctcggcgag cagcgcgtgg tcgacgcggc
     6241 gcatcaccag cagctgcatc gcgtccaccg ggctggcgac gtagcgcagg ttcaggtcct
     6301 tcctcacgtc catgccctgc ccttcggcaa ggagttgcag cacgatgtcg ggcatgtcgc
     6361 cgcgaaacgg catcgccagc tccttgcccc gcaggtcggc cagcgacttc agcgccggat
     6421 cgcgcgacac cagccacagc acaccccagg tcgacacgtt gatcagctgc agtttcgcgc
     6481 cgcggttgta gaggttggcg gcgacgttgg tcggcatcgc cagcacgtcg gcgcgcccgt
     6541 cgagcgccat cacgcgcaac tggtcggggt cgcgccacgg cacgaactcg accacctcgg
     6601 cgaggtcggc gagcgcaccc gagtcgacca tgtggatgag cgcgttcgac accgcggccg
     6661 acggaccggc cagggtcagt ttggcaagct tctgctcctg cgcatggacg atgccggtca
     6721 cgagcaggaa ggcgcacacc acgcagacgt gcatgaccgc gcgcatccac gccgggggcg
     6781 ccatcgggcg gcgacgatcc tggcccatgt cagaagctcc cgttcaggct gagcgccacg
     6841 ccacggccgg gcgcggcgag ttcgttgccg gacacgccct cggtctggtg ctcgtgatag
     6901 cgcttgtcgg cgaggttgct cagttgcacg gccagcccgg cggattcgaa ggcgccgagc
     6961 ttgccgaagc gccagccgaa ctcggcgtcg gcggtgacga agccggcggt ggggttttcg
     7021 gtgccgttgg agaagcgggt cgcgatgcgg tcctgctcgg cgaccgcgcg caattgggcg
     7081 cgccaccaga agccacgttc ggccgcctgg ccaagcccca gggtcagctc gggcgcaggc
     7141 atctggaaca gcggctcgtc gtcctgatgg ttctcgccgc gcagccaggt gaagcccgcg
     7201 tcggcgacga aggcgccgac cggcatgctg gcgctgccct cgatgccgta gatcgtgacc
     7261 ttgtcgaggt tctcggtgag cttgaagggc aggccgttct gtggatgcac ggccccggtg
     7321 acacggccgg cgatgtagtc gtcgatgcgc gtatagaacg cggcaagctg gtaccgcagg
     7381 ccgacgccgc cccgcttcag gccgagctcg agactggtcg acacctccgg gtcgagctgc
     7441 gggttgccga catggaagta gccgtcgccg cgcgccgaat cctcgaagcg ctcgcgcatg
     7501 tccgccgcgc ggtaggcgcg gccgaggttg gcgtaggcgc tcaggcgctc gctcagcggc
     7561 tggatcgcac cgagcgacca cgacaggttg ttgtcggcgt tctccagccc ggtggtctgc
     7621 gtcatgccct tgccttcagc gtcaccggtg acgcgatcga aacgcgcccc agcgacgatc
     7681 cgggtatcgc cgagactgaa ctcatcctgc acgaagacgc ccgccgactc gatctcgccc
     7741 ttgtcgaaag gatcgttgcg caggttgttg tcgaaggccg gcggattgtt gtcgatgtag
     7801 cgctccggat cgccgcgcat gcgccagagg tcggcaccca cggtcagcag gtggtcggcg
     7861 ccgagcggga acaggtactt cgccttcgcg ccatcggtga cgaagcggac atcgttacgc
     7921 acatagtcgc gctgcttggc cgacgagaac gcgcggatct ggcggaacac ttcctgacgg
     7981 tagagctcgg cggacagcgt gccctcgccc agcttcgcgt ccacgccaag ctcgtagagc
     8041 tcgcggcgct gctcgggaga atggatggtc gcggtgccga gcggcttggg gattccggcg
     8101 ccggtgcgcg ccgagcccgg ataccacaca tcctcgtcct catgccgctg caggttcacc
     8161 cgcagggtca ggtcctcgct cagcttgtgc ttgtacttcg ccagcagcgt gtcggactgg
     8221 taaccggtgc gatcctcgcg gccgtccggg ctgcggtagt cgtccacgtc acgcccggcc
     8281 acgcccagca tcagcgcgcc gtgcgcgctc gagcgcgtca gcagcgcggc accggcgacg
     8341 cccctgtcca cgctggagaa gcgcgcgccg acgcgcccgc ccaccgcctc ttcggcgctg
     8401 aagccgacat cgggcgtgat caggttgacc accccgccca tcgcgccgct gccgtacagc
     8461 accgagcccg gccccttgac cacctcggcg cgctcgagca ggccgaggtc gaggaaggag
     8521 gcgatcgcgc cctgcggctg cgccgagttc atgcgcacgc cgtcgaccat caccaccacg
     8581 ctttccttct tgagcccgcg cagcaccggg ttctgccccc aggcaccgtc cgactgcgcc
     8641 gccaggccgg gcttgccgcg cagcagcgcg cccgcgaccg tgccgccggc cgccggcgcc
     8701 tgcaggatct cgaccgcctg cggggtctcc agggcgtcgg cggcgtagcc cttggcggtg
     8761 acggtctggg cgccgagcag ggtctcggca tggacgaggc tggaaacggg gaaggcggac
     8821 aggccgagga cggccaccgc gagcgggcgc agacgaagca cgataaaaag ctccaggaag
     8881 tcgggttccc gggctggctg gcgacggctg gcttgcccga acaggaagat cagggagcga
     8941 tattatcgta atgcgaattg ttcgcacttg cgctttatca aattccgccc gcgcgacccg
     9001 gcttttcgcc agccgctatc atgcgtacgc aaagcctccc ctcgatctct cccgcccccg
     9061 ccgatgaagc tctctcgcct ctaccgcccg caagaccgcc gcttctggct gatgatcgtg
     9121 ctcaacctgc tctcggccat ccttgcctgg ctcctgcgca gctggccgct ggttccgctg
     9181 gcgaccgtcg tggtggcgct gttcgcgctc ggcaacgccg cgctcggaat gttcatcgcc
     9241 tgggatctgc tgcgcgacga gcgcgaggat tgagccgact ggccgctggc cccagtcagc
     9301 gggaagcggt acatcgcggc ggccatattc gcaccttgcg gcgctcctac cgaaaaccgc
     9361 cacggtccct gccgatgcag gagcgccgca aggcgcgaat gcggcggccg aggggcctcg
     9421 cgggttcaga tgtccggcat caggtagatg cgctcggcac ccgccggaac cggcgccgat
     9481 tgcggtcgaa cggacgcggg cctcggtgca ggcagcgccg gcagcggagc gagcgcgtgc
     9541 gcgcccagcg cgccccactg cagcacctcg atgcggccgt cgtggtgctc gacgacggcg
     9601 ctgcagctat ccacccagtc gccgcaattg aggtagcgga tgtcgccgat gcggcgatcc
     9661 gaggcccagt ggatgtggcc gcagatcacc ccgtccaccc cgcgctgctg cgcggcgtgg
     9721 gcgaccgcat cctcgaagtc gaagatgaag ctcaccgcct tcttcacctt ctgcttggcg
     9781 tagcccgcga gcgaccagta tcccggcacg ccgagcaggc gacgcgcacg cgacagcagc
     9841 aggttgatgc gcaccagcgc gttgtaggcg acgtcaccca gcagcgccac ccagcggtgg
     9901 tggcgggtga cctggtcgta ctcgtcgccg tgcaccagcc agtagcgccg gccgtcggcg
     9961 gtgtggtgga tccattccga ctcgacgcgg atgtcgccga aggcgacgcc ggcatgctcg
    10021 cgcaaggcct cgtcgtggtt gcccggaatg aagatcacct ggcagccgtg gcgggcgcgg
    10081 cgcagcacct tctgcaccac ggtgttgtgg ctgggcgccc actgcacgct gcgcttcatc
    10141 gcccagaagt cgacgatgtc gccgagcagg tagagatagt ccgactcgtg ctcgcgcagg
    10201 aagtcgagca ggcgctcggc ctgacaggca cgggtgccga gatggacatc ggagaggaag
    10261 atcgcgcgca cgctgctcat ggttcaggtc tcctccgggc ggggttcggg caaatcgagg
    10321 ggccggccgc tgcccgcccg ccacacgcgc agcagggccg cggcgaagcg gtcgttcacc
    10381 cgctcccaat cgatcgcctc ggcgctgcgg cgcgctgcgc gcgcgagctc cgcgcgcagc
    10441 gcatcggccc cggccatctg tacggcacgc gcgatgaagc cctcttcgtc gccacaggcc
    10501 acgctggcac cgttgccgag gtcgcggatc acctcggcgg cggcggcgta gtcgtaggcc
    10561 accgggcaca cgccgctcgc catcgcctcg agggtgacgt tgccgaaggt ctcggtgaga
    10621 ctggggaaca ggaacagatc ggccgacgcg tagtgcgcgg cgaggtcctc gccgtgacgc
    10681 atgccgacga agacggcatc ggggtgggcg cgcgccagcg ccgcacgctg ggggccgtcg
    10741 ccgaccagga ccatgcgcgc gtccgggcgc aggcggcgga tcgctgcgaa ggcgcgcatc
    10801 gcaaggccga ggttcttctc cggcgccagg cggccgacgc tgatcaccgc cagcccgccc
    10861 gggggtaggc cccaggcacg gcgcaaggcc tcgtcgcggc gcgccggcga atacagcgcg
    10921 gtgtctaccc cgcgcgagat cacttccacg ctgcggtaac cctgcgccga gagttgcgcc
    10981 gcgagcgccg cggttggcac gaaggtcacc gcgctgcggt tgtggaagcg gcgcaggtag
    11041 gccgccaccg gctggcgcag ccagcccatg ccatagtggc cgctgtagtg gtcgaaattg
    11101 gtgtggaagt cggagctgac cgggatgcgc agcttgttgg ccgcggtgac cgcggtccac
    11161 ccgagcggcc cctcggtcgc cacgtgcacc aggtccgggc gctggcgcga ccactcgcgc
    11221 acgagacgca cccgcgccgg cagcccgagc ttgaggccgt cgtagcgcgg caggcgcagg
    11281 ccgcgggcga gcacctcttc caggccctcg ccgtgcgccg cggtctcccc ggggcgctgg
    11341 cgcgggcgga tgagctgtac gccgtggccg cggcggatga ggccgtcgac catccgcccc
    11401 agggtcatgg ccacgccatt gacctcgggc gcccaggtct cggtcacaag cgcgatgcgc
    11461 aggcgcggcg cgcggcagag gtcttcggtg gtgtagtcga gcgttctcat ggccccgcag
    11521 actagggggg ccgcaagaca gcggtgttac gccggcattg ccgcgagctt accgcggcgc
    11581 gtgccggggg ggcacagctt cagcgcgccg gcgcgggcgc atcgagcgcg gccacgatcg
    11641 ccgcgtcgta gccgtagatg tcgttgcgca gcagcaccct gcccgcctcg tcgagcgccg
    11701 cggtggcgta gtgcagcaac accttgacgt cgcgccccac gtccacggtg cgcgtgcgcc
    11761 cgctgtcgag cgccgcctgc agcgcctgcg cgctccagcg cccgctgtcg gcgagcagca
    11821 gcaccgccag ctcctcgggc ttctccacgc gcacgcagcc cgagctgagc gcacgctcgg
    11881 cgcgcttgaa cagggagcgc gcgttggtgt cgtgcaggta gatcacgtag gggttggaga
    11941 gcgagaactt gatccgccca agcgagccgt cggcaccgga ctgctgctcg acgcgatagg
    12001 gaaaaccgct ctggcgcgcc ccgctccagt cgatggtggc ggggtccacc gtctgcccgc
    12061 tgcgatcgac cacgcgcagg cgccggttgg cgagatagga cgggttgcgc gctgcgcccg
    12121 gaatcacgtc ctcgcgcagg atggtgggtg gcaccaccca cttcgggttg agcaccagat
    12181 gggtgacgct gtcgagcagc gagggggtgc gccgcttggg cttgccgacg atcacccgcg
    12241 aggaccacac cggctggccg tcgagcacga ggtcggcgtg gtagccgacg atgtccacca
    12301 gcaggcggtc gccctgcagg tcgctcgcca cccagcgcag gcgctcgagg ttggcgcgga
    12361 tctgcgccac gcgctgcgcc gggctggctt tgagcgcctc cagggtctgc gccccgagca
    12421 cgccgtcggc ctgcaggccg tgcgcggcct ggaagcgctt gaccgcctcg accaaagctt
    12481 cgtcgtagtg ggacttgtcg cgcgcctcgg acacgcgctc gcccgcggcg gccaggcgcg
    12541 cgcgcaacga ggccacgcgc gagctgcttg caccgggctt gagggtgggc ccggcagcga
    12601 ccttgggcca gtcgcccacg gcgagccggc cttgctgggc gagcagctcg gcgcgcagcg
    12661 cgcggtagag cggcaggtcc ggcgcgtacg cgtcgaccgc ggcggccaga tcgggcgcct
    12721 gcagcacgcg tgcaagctcg gccgcacgct cgtaggggct gggcggcggg gtgaagttcc
    12781 actcgcggta gagcgcgcgc gggtcgacct tgccatggcg gagctggaag agcagcgcgg
    12841 ccagggtgtc ggtgagcaag agctcgcgct cggccgcacg ctcgggatcg gccgcgggga
    12901 cggcaccggc gcgcaggcgg gcgggggcga aatccgccgc atccagccca tgcccgcgta
    12961 ccgcctcgac cgccgcgagc agggcgtgca cgcgcgcagg ttcggtccac accgaccggt
    13021 aggcacgcgc gaggtagaac agcgcgatgg ggtcgtcggc cgtcgcggcg cgctcgcgca
    13081 ggcgctgctc gatgcgcagc gcgagcgcgg agtcgctcgt ggccggctcg accgcgacca
    13141 ccgcggcctc gccagccgct gtcgcatgca cgccggacgc catgagggcc agcgcggcga
    13201 gggccgcggc agttacaaaa catttgccca gcccgggctt gctttgcatt aggattccgg
    13261 cctcaagtac ttgttcggat tgcttattat ccatgtctaa gatcgtccgg aatcaggtcg
    13321 tgccgcaccg ccgcctgtta ctcaagggcc tcgccacgct gccgctgggg ctttcggccg
    13381 ccgcccaggc cgcccagcgc gacccgcaac tcgacttccg ccacactcac accgacgagc
    13441 gcctgagcgt cgccttccgc aaccgccagg gttacatcca gcccgccctg cagcgcatcg
    13501 actggctctt gcgcgacttt cggaccggag agtctacacg gatggatccg cgcctctatg
    13561 acatgcttca cgcattgagc ctcgcgtgcg gcggaaacac cttcgagatc atctcgggct
    13621 accgctcgcc gaccaccaac accatgctgc gcaagacacg cggcggcgag gtggccagac
    13681 gcagcctgca catggaaggc aaggcgatcg acatccgcct ggtgggcgtc gataccgccc
    13741 gcctgcgcga cgccgcgctc gcgctcggcg gcggcggcgt cggctactac cccgactccg
    13801 acttcgtgca catcgacacc ggtccggtgc gcagctgggg tccgcggtcg gcctgatccc
    13861 gtcgcacccg gcccgcatcg gccctttcag aacagcagca agccccacac cacggcagcg
    13921 tggagcagtg cgagcagcac cgccgcgctg ccgacgtcct tcgcgcgctt ggccagctcg
    13981 tgccgctcca gcgacacccg gtcgaccgtc gcctccaccg cagagttcag caattccacc
    14041 accagcacca gcaggacgct gccgatcatc agcgcgcgct cgaccgcacc ctcgcccagc
    14101 cacagcgcga gcggaaccgc gaccagggtg agccatattt cctggcggaa ggcatcctcg
    14161 tgccggtagg ctgcgcgcag tccgtccatc gaatagcggg ccgcgttgaa gatccggcgc
    14221 agaccggtct tgcctttgaa gggactttcc atcctggctc atccatccgt gctcgaaagg
    14281 ttcgcgatca tgccacgaaa gcgcggcacg acccgcgccg tggcgcggct ccgacgctcc
    14341 cgatccggcg ccggcacggc ggcggagggg gcacgatgac gcgcacgatc ggtcgcttcg
    14401 agatccgccg cgaactcggc cgcggcgcac agagcgtggt gcacctggcg tgggacccgc
    14461 agctcgagcg cgaggtggcg ctcaaggcgc tgcacctcgg cccccgcccc ggccacgaca
    14521 acgcctcgct gctcgccgag gcgcgcgcgg tgagccggct gcgccacccc ggcatcgtcc
    14581 cggtgttcga ggccggcgag gacggcggcg atccttacct ggtgttcgag tacgtgcccg
    14641 gcgaaagcct ggccgcgctg ctcgaccggc gcgggccgct gcctgccggc gaggccgtgg
    14701 agcgcatgct ggagatactc gccgcgctcg ccgaggcgca cgcgcagggc gtgatccatc
    14761 gcgacctcaa gcccagcaac gtgctgctcg acggcggcgg ccatgcgcgc gtgatggatt
    14821 tcggcatcgc ccgccgcatc gaggccgcgg gggccagcga gggcctctcg ggcaccccgg
    14881 gctacatggc gccggagtac atcgagcgcc gcgagatcgg cccgcgcaac gacgtcttcg
    14941 ccgccggcgt gctgctgatc gagctgctcg ccggtcgtcc gctggcgcgc gagggcggcg
    15001 tgaagagggt cctcgagcgc acgcgcagca cacccgtcac cctgcccgcg gactgccccc
    15061 cggtcgacga ggccctggcc gcgatcgcgc tgcgtgccgc cgcgctcgac ccgctcgcgc
    15121 gctttgccga cgccgccgag ttccgcctcg cgctcgaagc ctggcgccag cccgccgaga
    15181 ccgacccgga ggccggcagc gaggccaggc cgggcgcggt ggatttcctg atgcgccgca
    15241 tgaagcaccg cggcgacttt cccgcactct ccgaatcggt agtggcgatc aaccgcatcg
    15301 cggccagcga atcggagagc gtgggcaccc tgtcggcact gatcctgcgc gacttcggcc
    15361 tcaccaacaa gctgctgcgg gtggtgaact ccgcgcattt ccgccccgcc ggcggcgggc
    15421 gcatcagcac gatctcgcgc gcggtggtgg tgctcggctt cgatgcggtg cgcaacatcg
    15481 cgatcaccgt gctgctcttc gagcacctgc agaacaagtc caacgccagc caactgcagg
    15541 aggagttcct gcgcgcctgc ctggcgggcc tgttcgcgcg cgaactcgcc cggcgcctgc
    15601 gcctgcgcga cggtgagcag agctacatct gcgcggtgtt ccacaatctg ggcaggctgc
    15661 tgtgccagtt ctacttcccg gaggagagcg aggacatccg ccgcgtcgtg cagcagcacg
    15721 gctgcagcga ggagagcgcg gcgcagcgcg tgctcggcgc cggctacgag gaactcggca
    15781 tcgcgctcgc gcgcgcctgg ggcttccccg agctgatcca gaacagcatg cgcaccctgc
    15841 ccgagggccc ggtccatgcg ccgcgcagtg ccgacgagcg cctgcagagc ctggccgcct
    15901 gcgccaacgc gtgttgcgat gcggtgtcgc gcctcgcccc cgcggagcgc gagcgtgcgc
    15961 tggccgacat cgccgcgcgc ttcgaagagg ccgtgccggt gcctgcgcgc gaggcgcgcg
    16021 agcacctgcg cagcgcggtc gacgagatcg gcgaattcgc ccgcgtggtc aaggtaagcc
    16081 tcgcgcagac gcgcttcggg cgcaacctgc gtcacttcat cgacggcagc acgccggccg
    16141 agggcgcacg ggtcgacgcc acgctgatgc ccgcctccgc gctgctcgag cagtcgctgc
    16201 ccgccgaggg cggtgcggaa ggccagcgcg ccgccgaccc gcaggccgtg ctgagcgcag
    16261 gcatccagga catcagcaac agcttggtcg aggatttccg cctcaacgac gtgctgcgca
    16321 tcatcctcga gaccatgtac cgcgcgatgg gcttcaagcg cgtgctgctg tgcgtgcgcg
    16381 acgcgcgcag caacgccatg tgcgggcgct tcggcttcgg ccccgaggt
//
LOCUS       DAOQNN010000026        16233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1397_length_16233_cov_10.709358, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000026 DAOQNN010000000
VERSION     DAOQNN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 16233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16233
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1397_length_16233_cov_10.709358"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..940)
                     /locus_tag="PKV97_03120"
     CDS             complement(26..940)
                     /locus_tag="PKV97_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB04897.1"
                     /translation="MRRISLEDLHIFRCVAVEGGVLRAADRLNRVPSNVTTRIKQFES
                     RLGTQLFRRQGRNVVLTDAGHRLLGHAERLLRLADEAEEDLCSGVVAGSLRLGSLESA
                     ASVRLPPILSRYHAAHPRTHVELQTGTTAALLRRLENHRIDAAFVSEPFDKGSLSAVA
                     VFAEELVLITARGGPRIGGAQDLADQTVVAFPHGCSYRRLLVDWFAAAGVTPRRFLDL
                     GSYHAIVACVAAGTGVAIMPASVLDNAVMGDSIQRHELPEAMRTNRTNLVWAGEPGGP
                     LSALLELLRVGAQAGACGRHGRTSKSMP"
     gene            1080..2213
                     /locus_tag="PKV97_03125"
     CDS             1080..2213
                     /locus_tag="PKV97_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HNB04898.1"
                     /translation="MGIGRFAFTPLMPLMVRDGSLNTVTGTEWATANYVGYLLGAISA
                     SWFARSPRLGLQVGLLGVAATTLAMAWGNAALPWFGLALRASAGVFSAWVLVCASSWC
                     LPELARRSSTSLGGWIYTGVGVGIAATGVLTWLGGAQAAAVLWVELGLLAVVGAVHVI
                     WRLRGQSAAASPAGTPDAPKPPPPAVASGNLGIVLCYSAFGFGYIIPATFLPTMARQL
                     VSDPLVFGLTWPIFGAAAALSVAFAAHRLHGWPRRKVWAIAQGVMAAGTLLPLLQPAL
                     WVLAASAVLVGGTFMVTTMAGLQLARERAPANPTALLARMTTGFAAGQIAGPLLVRLV
                     GDTRIAGLDALGLASATAGLLLAMTAIWLWRDDIAAATKPRSA"
     gene            2378..2764
                     /gene="sdhC"
                     /locus_tag="PKV97_03130"
     CDS             2378..2764
                     /gene="sdhC"
                     /locus_tag="PKV97_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNB04899.1"
                     /translation="MDKPPHRLPVFLNLAQIRFPIGAIASIAHRVSGVLLFIALPVLA
                     ALLHASLRSEADFASVRGLLSSPLSLVVAGVLAWALAHHVLAGIRHLLMDVGIGSELE
                     RARASARLILVAAPALALLLLVWGLS"
     gene            2761..3102
                     /gene="sdhD"
                     /locus_tag="PKV97_03135"
     CDS             2761..3102
                     /gene="sdhD"
                     /locus_tag="PKV97_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNB04900.1"
                     /translation="MRLFTGQRAWVVQRLSALLLLVLILLAMLALPFASPLDFSAWRA
                     LAGGALGGPLIALLFGALCAHAWVGMRDIVLDYLQPRGLRLAVLGLITVVLLGVLARV
                     LLSLAMVALAA"
     gene            3120..3815
                     /locus_tag="PKV97_03140"
     CDS             3120..3815
                     /locus_tag="PKV97_03140"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNB04901.1"
                     /translation="MRLNIYRYDPERGDPPRMQAYDVQPEAGDKKLLDLLMRLRVQDD
                     SLSFRRSCREGVCGSDAMNINGRNGLACLTALDGLKEPIVLRPLPGFPVIRDLVVDMT
                     QFFAHYHSIRPWLINDEPLPERERLQTPAERERLDGLYECILCACCSAFCPSYWWNPD
                     KFIGPSGLLQAYRFIADSRDTASAERLDFLDDVYRLYRCRTIMNCTEVCPKGLSPSHA
                     IERIRLALLQRSS"
     gene            complement(3873..4448)
                     /locus_tag="PKV97_03145"
     CDS             complement(3873..4448)
                     /locus_tag="PKV97_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1282 family protein"
                     /protein_id="HNB04902.1"
                     /translation="MSSLHLPPMLWSSDAAWTELSRRPPSLATLVFGVALPAAMLPPA
                     MLSHATDVGSAHYLPALSASAWQFVEFALFAAQLVGFLMIRWLIGAVAATHGVRASAR
                     DACLLATVSLLPLWLSSFALLQDGTLAVLVLPVLAIGASVALLKRGVRCMLGVREEIV
                     ALEIGIVAIHCGVLAWAATMAVFLVPGLPAL"
     gene            complement(4631..5068)
                     /locus_tag="PKV97_03150"
     CDS             complement(4631..5068)
                     /locus_tag="PKV97_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNB04903.1"
                     /translation="MPERRITIKEVAERFDISRSHLMKIVTELVAKGFVDGARGKGGG
                     LRLARASSEIGLGNVVRQLEPSLALVECFGTQSHCLLDPACRLKGALDGALQAFLAAL
                     DRVSLADLVGSDARLHEALGLQAAARAIAPITIVRPPAGRERR"
     gene            5393..7069
                     /locus_tag="PKV97_03155"
     CDS             5393..7069
                     /locus_tag="PKV97_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04904.1"
                     /translation="MAEHAAEPTVKHFRLALLWPLRLLTATGALGPQQRPWQVLRDLG
                     EASPWREVEHDLEVEGSVFQERHYNEFVTFLPYVQQFLYGEGRAQRGDSADAGPAMRV
                     FRRRDIAKLRVAARDGDAPVTLDVVHVHLYFFFDVDVVILNVEVAGGDLPLSAVQELM
                     YRIGRAYPSGWDEHGRALHSLAVAELIGRDGSVLARTDAHEREAFLTHVSRHRAPRIA
                     AHWAWLMQPLASAYSAQPGTLRYRQIEYHRMPLTAYLALDDPQALTRRDFVRLGLVTG
                     AGPHTAQAEREGGGNALPYAEPHLDDFERRHCYDRFWSASGAAPWTRYLCSGHALVVV
                     GDAKSAYFTDRERGVLAQFRHQHFLLFLIAHFQKSALLMFSERLVEALKRLHVTDPES
                     VRRFKRAIRASFEGFLRFTHRYWFHDIAEQAQTRALFRMTSTHLGTDALYAEIKERTA
                     DMNGYLEADSLRRQANTVVRLTVVTIFGLIGTITTGFLGMNLLAEAESPLAERLAYFA
                     LVFGVSTALTLYTMVKSKRLSDFLDAVSDERLSTWNKLRALAAVWRRGAD"
     gene            complement(7079..8227)
                     /locus_tag="PKV97_03160"
     CDS             complement(7079..8227)
                     /locus_tag="PKV97_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNB04905.1"
                     /translation="MAIAVVLRSLLLLACLLFAHASPGADAGTAAPALDLPAPGQLHV
                     YVREGCPHCAEAKRFLAGLADARPGLQVVLREVDRDPVARDALMAISAATGAWPPGVP
                     TFVYGGRVLVGFDDAEHVGAELLRLLDEAPPPRRDRVESGLFGTLSASELGLPLFTLA
                     LGLLDGFNPCAMWLLLFLLSLLVRLQDRRRMAMVAGTFVLASGAVYYAFMAAWLNVFL
                     LVGMTAALRVGLAVLAMLIGLINVKDFFAFRRGVSLSIPESAKPGIYARARAILKAES
                     LPASLAAVAVLAVVVNFVELLCTAGLPAIYTAVLTSQDLSPLAHYGYLGLYILAYIAD
                     DALMVGTAVLALGSGKLGERGGRMLKLLAGVVMLALGAVMLLRPEWLM"
     gene            complement(8257..8829)
                     /locus_tag="PKV97_03165"
     CDS             complement(8257..8829)
                     /locus_tag="PKV97_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04906.1"
                     /translation="MNASRLLLALAASVLVVACAPEPAPAPKVEQAAPAPEAPRGEAA
                     EARREAAEAAREAMQKAGEAARKIGEAGSAAVEAAVRTAAEKAASEFAKDASRESSDA
                     GALNQAPDEDRGGDGRSPETVRDAKEAAEQAAHRIAEATRDAAERLREVGKGALESMR
                     ARTEPTTQEAAPVEERAAVPPGGGKEPAGR"
     gene            8828..8980
                     /locus_tag="PKV97_03170"
     CDS             8828..8980
                     /locus_tag="PKV97_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04907.1"
                     /translation="MKDESGFDGDSLQGTQSRREEELQDARRNKRKNDGAHMGPIVSV
                     TPEPLT"
     gene            complement(8973..9530)
                     /gene="efp"
                     /locus_tag="PKV97_03175"
     CDS             complement(8973..9530)
                     /gene="efp"
                     /locus_tag="PKV97_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNB04908.1"
                     /translation="MKTAQELRSGNVIMVGSDPLVVQKAEYNKSGRNSAVVKMKFKNL
                     LTGAPSESVYKADDKFEVVVLDHKEVTYSYFADPMYVFMDADYEQYEVEADNMTDALK
                     YLEDGLQCEVVFYNGKAISVELPNSVVREVVYTEPAVKGDTSGKVMKPAKISTGFELP
                     VPAFVEIGDKIEIDTRTDEYKNRVK"
     gene            complement(9596..10783)
                     /gene="earP"
                     /locus_tag="PKV97_03180"
     CDS             complement(9596..10783)
                     /gene="earP"
                     /locus_tag="PKV97_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HNB04909.1"
                     /translation="MHRFRSPARPRWEIFCQVVDNFGDIGVCWRLATDLARRGGGAVR
                     LWVDEWEALRRICPAAAAVDERAGGSVQGVELRRWTSAFVPLVPGEIVVEAFACELPA
                     AQLEAMAALRQAPLWINLEYLSAEAWVAGCHGMASPHPRLPLVKHFFFPGFDAGTGGL
                     PREADLLARRDAWRARPEGRAAWLAARGIASSPDALRVSLFAYEQPELPALFDAWTGS
                     GREMLVLVPESRVLGDVQRALGRERLQAGDRVVRGALTACVLPFTDQAGYDELLWACE
                     LNFVRGEDSFVRAQWAARPFVWQIYPQADAAHHDKLEAFLARYLQGLPAAEADALARF
                     WRAWNGCAPDRATPAEAWPALAAALPGLDVHARRWCDVQAALPDLATALDTFCSHIAH
                     AAR"
     gene            complement(10893..11132)
                     /locus_tag="PKV97_03185"
     CDS             complement(10893..11132)
                     /locus_tag="PKV97_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04910.1"
                     /translation="MGLAASRVVHGRGSDHPWPASCRHSRALGKLGGPAVAAAGLFFC
                     FSIDLNNKEVNSGAFLVDKYPETLPAKRSCSSKGG"
     gene            11151..12113
                     /locus_tag="PKV97_03190"
     CDS             11151..12113
                     /locus_tag="PKV97_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04911.1"
                     /translation="MHGRKLLPLALILAFPALAIAQSAQQQGLPSPEAAQEQALLEQS
                     RTQVRGFSVWLGETVNDWFGDRPFEEGGKVTNGRLGLRNLWRQDEGWNTNVRFRARFD
                     LPNLRDKAYVFIGRQNERELVTDQSEAFTREQLLLEERRKEDQTGFAGVGVALRKNLD
                     FRVGVRGGMKFYTQARYRKQWALTELDRIEFRETVFWNNKDKFGSTTALDYEHAFTPS
                     LSLNWQNATTISEKTDDFAWGSSLGLFKRFGDDRILSGEMLVTGETGAEENVDEYGVR
                     MKWTQPVYRDWLIGEFIVGHYWPQREDRERERAWGVGVNLEMRF"
     gene            complement(12377..13123)
                     /locus_tag="PKV97_03195"
     CDS             complement(12377..13123)
                     /locus_tag="PKV97_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HNB04912.1"
                     /translation="MRPMQLERLLHAQGFGSRKDCRALIRAGYVSVAGEVCDDPTEEF
                     DPGSRDGSPGLEFTVDDQPWRFRAQAYVVLHKPAGYECSQKPTFHPSVFTLLPPQLLA
                     RGVQCVGRLDQDTTGLLLLSDDGQFIHQWSSGKKRTPKVYEVTLKHAVEQDFADRLVA
                     GVELNDEPVPIAAAACELVSPTTLRLTICEGKYHQVKRMVGAAGNRVEVLHRSRVGGL
                     DLPAGLAPGQWRWLEAEDLTRLADYADATS"
     gene            13251..13718
                     /locus_tag="PKV97_03200"
     CDS             13251..13718
                     /locus_tag="PKV97_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB04913.1"
                     /translation="MKFEHLIEVNDLANPLLTPLTREELWFGLLCRAEDARAFLPGLE
                     ACRIVERGEGSLVRDLHFGHAVIRDRVNFDTLRWIRFETEANAQHAGGSLTITIEEPE
                     PGALFLRFAYHTSLPDQGEDARYAEFVRSAYHQSDLDTVRIIRMIAASGGLEQ"
     gene            13910..14920
                     /locus_tag="PKV97_03205"
     CDS             13910..14920
                     /locus_tag="PKV97_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HNB04914.1"
                     /translation="MWFKNLQVYRLPANWDMSAERLEEQLAKKPFHPCGSQDMESRGW
                     LSPLGNEVLVHAVGGQWLVCLGFEHRLLPSAVVKQEADERAEELAEQQGYKLGRKQLK
                     ELREQVTQELMPRAFTRRRRMFAWIDPINGWLVVDAASQSKAEDMLEQLRHTLDSFPL
                     TLLRTERSPMSAMADWLAGGEAPDGFTIDQDCELRSVAEDKAAVRYARHPLEGDDIKG
                     HLEAGKLPTRLALTFDDRISFVLTEKLEIKRLDFLDIVRDQVGEADKDDAEALFNAEF
                     ALMTGELAHLLPAVVAALGGELAGAAVETGVSAELPRVQKEMAAARPVSAQTVAADDP
                     PF"
     gene            15413..15868
                     /locus_tag="PKV97_03210"
     CDS             15413..15868
                     /locus_tag="PKV97_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNB04915.1"
                     /translation="MNDTMFLEWWHWEIAGIALVLLELALPSFFIIWFGLGAMLTGFV
                     LLAMPDLALSQQIAVWIIASMAMTVLWFSFAKRARVRTLIGTAAGEVIGEVGLLVSAV
                     APFERGKVRFQRPVLGADEWACVAESAIAAGERVKVVSVEGSYVKVTKA"
     gene            15889..>16233
                     /locus_tag="PKV97_03215"
     CDS             15889..>16233
                     /locus_tag="PKV97_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNB04916.1"
                     /translation="MIMSEGLAIAIAVLVFVVITIAKGVRLVAQGEEWVVERLGKYHA
                     TLRPGLNILIPYLDRVAYKLVTKDIILDVQEQEVITRDNAVILTNAIAFVKVTDPVKA
                     VYGVTDFSEAIRN"
BASE COUNT         2632 a         5560 c         5507 g         2534 t
ORIGIN      
        1 cgtcggcggg gatgccgggc gggcctcaag gcatcgactt gcttgttcga ccatggcgcc
       61 cgcaggcacc agcctgggcg ccgacccgca gcagctccag cagcgcgctc agtggcccgc
      121 cgggctcgcc cgcccagacc aggttcgtcc ggttcgtccg catcgcctcc ggcagttcgt
      181 ggcgttggat cgaatcgccc atgacggcat tgtcgaggac gctcgccggc atgatggcga
      241 cgccggttcc ggccgcgacg caggccacga tagcatggta ggagccgagg tcgaggaacc
      301 ggcgcggggt gacaccggcc gcggcgaacc agtcgaccag caggcgccgg tatgagcagc
      361 catgcgggaa ggcgacgacc gtctggtcgg cgagatcctg cgcaccgccg atgcgggggc
      421 cgcctcgcgc ggtgatgagg accagctctt ccgcgaagac ggccacggcc gagaggcttc
      481 ccttgtcgaa gggttcggag acgaaggcgg cgtcgatccg gtgattctcg agtcgccgca
      541 gcagcgccgc ggtcgttccg gtctgcagct ccacgtgcgt tcggggatgc gcggcgtggt
      601 accgcgagag gatgggcggc aggcgcacgc tggccgcact ctccagcgag cccaggcgca
      661 gcgagcccgc gactaccccg ctgcagaggt cctcctcggc ttcgtcggcc agcctcaaca
      721 ggcgctcggc gtgcccgagc aggcgatgac ccgcatcggt gagcaccacg ttccgtcctt
      781 gccgccgaaa gagttgcgta cccagtcggg actcgaactg cttgatgcgg gtcgtgacgt
      841 tggaaggcac ccgattgagc cggtccgccg cacgcagcac accgccttcc accgccacac
      901 atcggaagat gtgcaggtct tccaggctga tccttctcat atcgagaacg ttatttctta
      961 aaaattcact attcgattct atccgacgag cgtctaatcc cgccatggaa aaaaatcctc
     1021 cgtcgtctct gtcgccgggc gccatcgtct tcatcggcct ccttgcgctg gccatcgcga
     1081 tgggcatcgg tcgcttcgca tttacgccgc tgatgccgct catggtgcgc gatggcagcc
     1141 tgaataccgt caccgggacg gaatgggcga cggcgaacta cgtcggctac ctgctcggtg
     1201 cgatcagcgc ctcgtggttc gcgcgcagtc cccggctggg cttgcaggtc gggttgctcg
     1261 gcgtcgccgc gaccaccctg gcgatggctt ggggtaacgc cgccttgccg tggttcggct
     1321 tggccctgcg ggcgagcgcg ggcgtcttca gcgcctgggt gctcgtgtgc gccagcagct
     1381 ggtgcctgcc cgaactggcg cggcggagtt cgacctcgct gggcggatgg atctacaccg
     1441 gggtgggcgt gggcatcgcg gccacgggtg tactgacctg gctgggcggc gcacaggcgg
     1501 ccgcggtgct gtgggtggag ctcgggctgc tcgccgtcgt cggcgccgtc catgtcattt
     1561 ggcgtctgcg cgggcaatcc gccgcagcga gccctgccgg cacgcccgac gcgccgaagc
     1621 ccccaccgcc ggcggtcgcg agcgggaacc tgggcatcgt gctgtgctac agcgcgttcg
     1681 gcttcggcta catcattccc gcgaccttcc tgcccaccat ggcgcggcag ctggtgtccg
     1741 acccgctcgt gttcgggctc acctggccga tcttcggcgc ggcggcggcg ctgtccgtgg
     1801 ccttcgccgc gcaccgtctg cacggctggc cgcgcaggaa ggtgtgggcg atcgcccagg
     1861 gggtgatggc ggcaggcacc ctgctgccgt tgctgcagcc ggcattgtgg gtactggccg
     1921 cctcggcggt gctggtcggc ggcaccttca tggtgaccac catggcaggc cttcagctgg
     1981 cgcgcgaacg cgcgcccgcg aacccgacgg cgctgctggc ccgcatgacc accggcttcg
     2041 ctgccggaca gatcgccggt ccgctgctcg tgcgcctggt cggcgatacg cgcattgccg
     2101 gcctcgacgc gctgggcctg gcgagcgcga ccgccggcct cctgctggcg atgaccgcca
     2161 tctggctctg gcgcgacgac atcgcggccg caacgaagcc gcgatccgct tgagacgcgg
     2221 acgaacgccc gagcacgatc gcggcgtcgg attccgccgg gcccggcacc ccttgcttca
     2281 gcacgcatca cgcctgcgct gcgccgggct ttggtctatg gttccggtac gagccgctcc
     2341 ggcgccgccg gcgggctcgt actgcccaag agcccgcatg gacaagccgc ctcatcgctt
     2401 gccggtattc ctgaatctgg cacagatccg ctttccgatc ggtgcgatcg cctcgattgc
     2461 gcaccgggtg tcgggggtct tgctgttcat cgccctgccc gtcctcgcgg cgctgctcca
     2521 cgcctcgctg cgcagcgaag cggatttcgc ctcggtgcgc ggtctgctct cgtcgccgct
     2581 ctcgctcgtg gtggccggtg tgctggcgtg ggcgctcgcg catcacgtgc tggccgggat
     2641 ccgccacctg ttgatggacg tcggcatcgg cagcgagctc gagcgtgcgc gcgccagcgc
     2701 acgcctcatc ctggtcgccg cgcccgcgct ggccctgctc cttctcgtgt gggggctgtc
     2761 atgaggttgt tcacagggca acgggcatgg gtggtgcaac gcctcagcgc cttgctgttg
     2821 ctggtgctga tcctgctcgc catgctcgcg ctgcctttcg cgtcgccgct cgatttctcc
     2881 gcgtggcgcg ccttggccgg cggcgcgctg ggggggccgc tgatcgcgct gctgttcggc
     2941 gccttgtgcg cgcacgcctg ggtaggcatg cgcgacatcg tgctcgacta cctccagccg
     3001 cgcggactgc ggctggcggt gctgggcctg atcacggtcg tgctgctcgg cgtgctggca
     3061 cgcgtgctgc tgagcctggc catggtggcg ctcgccgcct gagcggcgca ggggcggaca
     3121 tgagactgaa catctatcgc tacgacccgg agcgcggcga tccgccgcgg atgcaggcct
     3181 acgatgtgca gccggaggcg ggcgacaaga agctgctcga cctgctgatg aggctgcgcg
     3241 tgcaggacga cagcctgtcg ttccggcgct cgtgccgcga gggagtgtgc ggctcggacg
     3301 cgatgaacat caacggccgc aacggcctcg cctgcctgac cgcgctcgac gggctgaagg
     3361 agccgatcgt gctgcgcccg ctacccggct ttccggtaat ccgcgacctc gtcgtagaca
     3421 tgacgcagtt cttcgcccac taccactcga tccggccctg gctgatcaac gacgagcccc
     3481 tgcccgagcg cgagcggctg cagaccccgg ccgagcgcga gcgcctggac ggcctgtacg
     3541 aatgcatcct gtgcgcctgc tgcagcgcgt tctgcccgtc ctactggtgg aatccggaca
     3601 agttcatcgg gccgtcgggc ctgctgcagg cctatcgctt catcgccgac agccgcgaca
     3661 ccgcctccgc cgagcggctg gacttcctcg acgacgtcta tcgcctctac cgctgccgca
     3721 cgatcatgaa ctgcaccgag gtctgcccga agggactgag cccctcgcac gccatcgaac
     3781 ggatccgcct ggccttgctg cagcgttcat cctgaaaagg cagggcggga gcgcgccatt
     3841 cgccccgggg gccggaccgc gttctttccg cgtcaaagcg ccgggaggcc cgggacgagg
     3901 aacaccgcca tcgttgcggc ccaggccagc acaccgcagt ggatcgcgac gatgccgatc
     3961 tccagggcca cgatctcttc acgaaccccg agcatgcagc ggacaccccg cttcagcagc
     4021 gcgaccgaag cgccgatcgc cagcaccggc agtaccagga cggcgagcgt cccgtcctgc
     4081 aggagcgcga acgacgatag ccagagcggg agcagggata ccgtcgcgag cagacaagcg
     4141 tcgcgtgcgc tcgcccgcac cccatgggtc gccgcgacgg cgccgatcag ccagcggatc
     4201 atcaggaagc cgacgagctg cgcggcgaac agcgcgaact ccacgaactg ccaggcggaa
     4261 gcggagaggg cgggcagata gtgggccgat ccgacatcgg tcgcatggga caacatcgcg
     4321 gggggcaaca tggcggccgg cagggcgacc ccgaagacca gggtggccag cgagggcggg
     4381 cggcgggaga gttcggtcca ggcggcgtcc gaggaccaca gcatgggcgg cagatggagg
     4441 gaagacatga tcgatctctg ctggcgtgtt tgggcggctc gaggaattta tatcacaaca
     4501 tgatataaac tagcatggcc cgccccccgg cagcgaatac gttgcgacca gcgcgcggag
     4561 aacccgcccg cagctttgcg cggccacgaa gcacaggcag accgtcatcc ggtggcgcag
     4621 cgcgccccga ctatcgccgc tcccggccgg cgggcgggcg cacgatcgtg atcggcgcga
     4681 tggcgcgcgc cgccgcttgc aggccgagtg cctcgtgcag ccgtgcgtcg ctgccgacga
     4741 ggtcggcaag cgaaacgcgg tcgagtgcag ccaggaaggc ctgcagggca ccgtccagcg
     4801 cgcccttgag ccggcacgcc ggatccagca ggcagtgcga ctgcgttccg aagcactcga
     4861 cgagcgcgag gctcggttcg agctggcgga ccacgttgcc gagaccgatc tcgctgcttg
     4921 cacgcgcgag cctcaaaccc cctcccttgc cgcgtgcgcc gtcgacgaag cccttcgcca
     4981 ccagttcggt gacgatcttc atgaggtggc tgcgcgagat gtcgaaacgc tcggcgacct
     5041 ccttgatggt gatgcgccgc tcggggatgg ccccgagcta catgagtacc cggagcgcgt
     5101 aatcggtgtg ttgcgtgatg ttcatcggca gggcgtgcag aggagtcgtt gtcggctcca
     5161 tgctagcaac aatagattca tacagattga ctcttcaagg aggaacggcc atgacccacg
     5221 tccttaccga agcctgcatc cgctgcaaat acacggactg cgtaagcatg tacccagtcg
     5281 agacccatcc ggcgctgccc gccaccgccc cctggcacgc tcgcagcccg cctgccctcg
     5341 cctgtcgcta cactgcccgc acgagaaccc gcgaagcgaa gggagacagg tcatggccga
     5401 gcacgctgcc gagccgacgg tgaagcattt ccgcctcgcc ctgctgtggc cgctgcggct
     5461 gctgaccgcg acgggcgcgc tggggccgca gcagcggccg tggcaggtgc tgcgcgacct
     5521 gggcgaggcc tcgccgtggc gcgaggtcga gcacgacctg gaggtcgaag gcagcgtctt
     5581 ccaggagcgg cactacaacg agttcgtgac cttcctgccc tacgtgcagc agttcctcta
     5641 cggcgagggc cgtgcgcagc gcggcgactc ggcggacgcg gggccggcga tgcgcgtgtt
     5701 ccgtcgccgc gatatcgcga aactgcgcgt ggccgcacgc gacggcgacg cgccggtgac
     5761 gctcgacgtc gtccatgtgc atctgtattt cttcttcgac gtcgatgtgg tgatcctcaa
     5821 cgtcgaggtc gccggcggcg acctgccctt gtccgcggtg caggagctga tgtatcgcat
     5881 cggccgcgcc tacccgtcgg gctgggacga gcacggccgc gcgctgcaca gcctcgccgt
     5941 cgccgagctg atcgggcgcg acgggtcggt gctggcgcgc accgacgcgc acgagcgcga
     6001 ggccttcctg acccacgtca gccgccatcg cgcaccgcgc atcgccgcgc actgggcctg
     6061 gctgatgcag ccgctggcga gcgcctactc ggcccagccc ggcacgctgc gctatcgcca
     6121 gatcgagtat caccgcatgc cgctgacggc ctatctcgcc ctcgacgacc cgcaggcgct
     6181 gacgcgcagg gatttcgtcc gcctcggcct ggtgaccggc gcgggaccgc acacggcaca
     6241 agccgaacgc gagggcggcg gcaacgccct gccctacgcc gaaccgcatc tggacgactt
     6301 cgagcgccgc cactgctacg accgcttctg gtccgcgagc ggcgcggcgc cctggacccg
     6361 ctacctgtgc agcggccacg cgctggtggt ggtgggcgat gcgaagtcgg cctatttcac
     6421 cgaccgcgag cgcggcgtgc tggcgcagtt ccgccaccag cacttcctgc tgttcctgat
     6481 cgcgcacttc cagaagtcgg cgctgctgat gttctccgag cgcctggtcg aggcgctcaa
     6541 gcgcctgcac gtcaccgacc cggagagcgt gcgccgcttc aagcgcgcga tccgcgccag
     6601 cttcgagggc ttcctgcgct tcacgcatcg ctactggttc cacgacatcg ccgagcaggc
     6661 gcagacacgc gcgctgttcc gcatgaccag cacacacctg ggcaccgacg cgctgtacgc
     6721 cgagatcaag gagcgcaccg cggacatgaa cggctatctc gaggccgaca gcctgcgccg
     6781 ccaggccaac accgtggtgc gcctgaccgt ggtgacgatc ttcggcctca tcggcaccat
     6841 caccaccggc ttcctgggca tgaacctgct cgccgaggcg gaatcgccgc tcgccgagcg
     6901 cctggcctac ttcgcgctgg tgttcggcgt gtcgacggcg ctcacgctgt acacgatggt
     6961 caagtccaag cgcctgtcgg acttcctcga cgcggtgtcg gacgagcgcc tgtcgacctg
     7021 gaacaagctc cgcgcgctcg ccgccgtgtg gcgtcgcggc gcggactgaa cggccccgct
     7081 acatcagcca ctccggccgc agcagcatca ccgcaccgag cgccagcatc accacgcccg
     7141 caagcagctt gagcatgcgg ccgccgcgct cgccgagctt gccgctgccg agggcgagca
     7201 ccgcggtgcc gaccatcagc gcgtcgtcgg cgatgtaggc gaggatgtag aggccgaggt
     7261 agccgtagtg ggcgagcggc gagaggtcct ggctggtgag caccgcggtg tagatcgccg
     7321 gcaggccggc ggtgcatagc agctcgacga agttcaccac caccgccagc accgcgaccg
     7381 cggcgagcga ggcgggcagg gattcggcct tcaggatggc gcgggcacgc gcatagatgc
     7441 ccggcttggc ggactccggg atggacagcg agacgccgcg gcggaaggcg aagaagtcct
     7501 tcacgttgat caggccgatg agcatggcca gcacggcgag gccgacgcgc agcgcggccg
     7561 tcatgccgac gagcaggaag acgttgagcc aggccgccat gaaggcgtag tacaccgcgc
     7621 cgctggcgag cacgaaggtg ccggcgacca tcgccatgcg gcggcggtcc tgcagcctca
     7681 ccagcagcga cagcaggaac agcagcaacc acatcgcgca ggggttgaag ccgtcgagca
     7741 ggccgagcgc cagggtgaac agcggcaggc cgagctcgct cgcgctcagg gtgccgaaca
     7801 ggcccgactc cacccgatcg cgccgcggcg gcggggcctc gtcgagcagg cgcagcagct
     7861 cggcgccgac gtgctcggcg tcgtcgaagc cgaccagcac ccggccgcca tagacgaagg
     7921 tgggcacgcc cggcggccac gccccggtcg ccgcgctgat cgccatcagg gcgtcgcgcg
     7981 cgacggggtc gcgatcgacc tcgcgcagca cgacctgcag cccggggcgg gcgtcggcga
     8041 gaccggcgag aaagcgcttg gcctccgcgc aatgcgggca gccttcgcgc acatagacgt
     8101 ggagctggcc cggcgcgggg agatcgagcg ccggcgcggc ggtgccggca tcggcgcccg
     8161 ggctcgcatg ggcgaacagc aggcatgcca gcagcaacag gctacgaaga accaccgcga
     8221 tcgccatgga tgcgcccctc gccggcgccg tgccgctcag cggccggcgg gctccttgcc
     8281 gcctccgggc ggaacggcgg cgcgctcctc gaccggcgcg gcctcctgcg tcgtcggctc
     8341 ggtgcgtgcg cgcatgctct cgagcgcccc cttgccgacc tcgcgcaggc gctccgcggc
     8401 gtcgcgggtg gcctcggcga tgcggtgcgc cgcctgctcg gcggcttcct tggcgtcgcg
     8461 cacggtctcg ggcgagcggc catcaccgcc acggtcttcg tcgggcgcct gattcagcgc
     8521 tcccgcgtcg gatgattcgc gcgaagcgtc cttggcgaat tcgctcgcgg ccttctccgc
     8581 cgcggtgcgc accgccgctt ccaccgcggc gctgcccgcc tcgccgatct tgcgcgcggc
     8641 ctcgccggcc ttctgcatcg cctcgcgtgc cgcctcggcg gcttcgcgcc gggcctcggc
     8701 agcctcgcca cgcggcgcct cgggcgccgg agcggcttgt tcgaccttgg gcgccggcgc
     8761 gggctcgggt gcgcaggcga cgacgagcac ggaggcggcg agggcgagga gcaggcggga
     8821 cgcgttcatg aaggacgaga gcggattcga cggcgacagc ctgcagggta cgcaatcccg
     8881 tcgcgaagaa gaactgcaag atgcacgccg caacaagcgg aaaaacgatg gggcccacat
     8941 gggccccatc gtatccgtca caccggaacc gcttacttga cgcggttctt gtactcgtcg
     9001 gtgcgggtgt cgatctcgat cttgtcgccg atctcgacga aggccggcac gggcagctcg
     9061 aagccggtgc tgatcttggc cggcttcatg accttgcccg aggtgtcgcc cttgacggcg
     9121 ggctcggtgt agacgacctc gcgcaccacg ctgttgggca actcgaccga gatcgccttg
     9181 ccgttgtaga acaccacctc gcactgcagg ccgtcctcga ggtacttgag cgcgtcggtc
     9241 atgttgtccg cttcgacttc gtactgctcg tagtcggcat ccatgaacac gtacatcggg
     9301 tccgcgaagt aggagtaggt gacttccttg tggtcgagga cgacgacctc gaacttgtcg
     9361 tcggccttgt agaccgactc cgacggcgca ccggtgagca ggttcttgaa cttcatcttc
     9421 acgaccgcgg agttgcggcc ggacttgttg tattcggcct tctgcaccac caacgggtcg
     9481 ctgccgacca tgatgacgtt gcccgagcgg agttcctgag cggttttcat gctgtttcct
     9541 gaatggttcg acgcgactcg tggtcgcatg tccttgaaaa aattagcgcg cgattctacc
     9601 gcgcggcgtg cgcgatgtgg gaacagaatg tatcgagtgc ggtcgcgaga tcgggcagcg
     9661 ccgcctgcac atcgcaccag cggcgagcat ggacatcgag cccgggcagc gccgcggcga
     9721 gggccggcca ggcctcggcc ggcgtcgcac gatccggcgc gcagccgttc caggcgcgcc
     9781 agaagcgggc gagcgcatcc gcctcggccg ccggcagccc ctgcaggtag cgcgccagga
     9841 aagcctcgag cttgtcgtga tgggcggcat ccgcctgcgg atagatctgc cacacgaagg
     9901 gtcgcgccgc ccattgcgcg cgcacgaagg agtcctcgcc gcgcacgaaa ttcagctcgc
     9961 aggcccacag cagctcgtcg tagcccgcct ggtcggtgaa gggcagcacg caggcggtga
    10021 gcgcgccgcg caccacccga tcgcccgcct gcaggcgctc gcgaccgagc gcacgttgca
    10081 cgtcgccgag cacgcgcgac tcgggcacca gcaccagcat ctcgcgaccg ctgccggtcc
    10141 aggcgtcgaa cagggcgggg agctccggct gctcgtaggc gaacagcgac acccgcagcg
    10201 catccgggct gctcgcgatg ccgcgcgcgg ccagccaggc ggcgcggccc tcgggacggg
    10261 ctcgccaggc gtcgcggcgt gcgagcaggt cggcctcgcg cggcaatccg ccagtgcccg
    10321 catcgaagcc cggaaaaaag aaatgcttca ccagcggcag gcgcggatgc ggcgaggcca
    10381 tgccgtgaca gcccgccacc caggcttcgg ccgacaggta ctcgaggttg atccacaacg
    10441 gcgcctgccg cagcgccgcc atggcctcga gctgggcggc cggcagctcg caggcgaagg
    10501 cctcgacgac gatctcgccc ggcacgagcg gaacgaacgc gctcgtccag cgccgcaact
    10561 ccaccccttg cacgcttccg cccgcccgct catccaccgc ggcggcggcc ggacagatgc
    10621 gccgcaaggc ctcccattca tccacccaga gccgcaccgc tccacctcca cggcgtgcca
    10681 gatcggtcgc cagccgccag cacacgccga tatccccaaa attatccaca acctgacaga
    10741 agatctccca gcggggacgg gcgggcgacc ggaatcgatg catggtgggg acaatcctgg
    10801 ataaacaatg gggacagctt gtgaacaagc cgacttcacg cgatcggccg gaaaaggctc
    10861 aacaaaacgc gcacagccga ccaaggggct tctcaaccgc cttttgaaga acacgaacgc
    10921 tttgccggca aggtctccgg gtacttatcc acaagaaacg cgccactgtt aacttctttg
    10981 ttatttaaat caatagaaaa acagaagaac aaacccgcag ccgccacggc cgggccacca
    11041 agcttgccca gcgcacgaga gtgccggcat gatgccggcc atgggtggtc gcttccccga
    11101 ccatgaacca cccgagaggc cgccaggccc atgaatcagg atccttcccc atgcatggcc
    11161 gcaaacttct tccgctcgcc ctgatcctcg cctttcccgc gctcgcaatc gcccaatcgg
    11221 cgcaacagca aggacttccc tcccccgagg cggcgcagga acaggctctg ctggagcaga
    11281 gccgcacgca ggtgcgcggt ttctcggtat ggctggggga aacggtgaat gactggttcg
    11341 gcgatcgtcc cttcgaggag ggcggcaagg tgaccaatgg gcgtctgggg ctacgcaacc
    11401 tgtggcgtca ggacgaaggc tggaatacca acgtgcgttt ccgtgcgcgt ttcgatctcc
    11461 ccaacctgcg cgacaaggcc tacgttttca tcggccggca gaacgagcgc gaactcgtga
    11521 ccgaccaatc cgaagccttt acgcgcgaac agctcctgct cgaagaacgc cgcaaggaag
    11581 accagaccgg ttttgccggc gtgggggtgg ccttgcgcaa gaacctcgat ttccgtgtcg
    11641 gtgtgcgcgg cggaatgaag ttctacaccc aggcgcgtta tcgcaagcag tgggcgctga
    11701 ccgaactgga ccggatcgaa ttccgcgaaa cggtgttctg gaacaacaag gacaagttcg
    11761 gctcgaccac cgcgctggac tacgagcacg ccttcacgcc ctcgctctcg ctgaattggc
    11821 agaatgcaac gacgatttcg gaaaagaccg acgatttcgc ctggggtagc agcctggggc
    11881 tgttcaagcg cttcggtgac gatcggatcc tgtcgggcga gatgctggtg accggagaaa
    11941 ccggtgccga agaaaacgtg gatgaatacg gcgtgcggat gaaatggacc cagccggtct
    12001 atcgcgactg gttgatcggc gagttcatcg tcggccacta ctggccgcag cgcgaggacc
    12061 gcgagcgcga gcgcgcctgg ggggtcgggg tgaacctcga gatgcgcttc tgagcttccg
    12121 aggcctcgta ggagcgacgc aagtcgcgat cggcgcgccg gttcgtgcgg cactcgtctc
    12181 gccaccgcaa tcgcgacttg cgtcgctccc acgggagaca gcgcggcgta tcggggccgt
    12241 aggagcgacg caagtcgcga tcgggcctcg atagcaccgt ccgctcgagg gcgagcgtcc
    12301 aatcgcgact tgcgtcgctc ctacggaaac cggtgcggag gccggcgcat gcggcggagc
    12361 gcgatggcag gcaggctcag gaagtcgcgt cggcgtagtc ggccaggcgg gtcaggtcct
    12421 cggcctcgag ccagcgccac tgcccgggcg ccagcccggc cggcaggtcg agcccgccca
    12481 cgcggctgcg gtgcagcacc tcgacacggt tgccggcggc gccgaccatg cgcttgacct
    12541 gatgatactt gccctcgcag atcgtcagcc gcagcgtggt cggactgacc agctcgcacg
    12601 ccgcagccgc gatcggaacg ggctcgtcgt tcagttcgac gccggcgacc agccggtcgg
    12661 cgaagtcctg ttccaccgca tgcttgagcg tcacctcata gaccttgggc gtacgcttct
    12721 ttcccgagct ccactggtgg atgaactggc cgtcgtcgga gagcagcagc aggccggtcg
    12781 tgtcctggtc gagtcggccc acgcactgca cgccgcgcgc gagcagctgc ggtggcagca
    12841 gggtgaacac cgagggatgg aaggtcggct tctgcgagca ttcgtagcct gcaggcttgt
    12901 gcaggaccac gtaggcctgg gcgcggaaac gccagggttg atcatcgacg gtgaattcga
    12961 gcccgggcga accatcgcgg ctgccggggt cgaattcctc ggtggggtcg tcgcagactt
    13021 cgccggcgac gctgacgtag ccggcacgga tgagggcacg gcaatccttg cggctgccga
    13081 agccctgggc gtggaggagg cgttcgagtt gcatgggacg catgttagca gggcgagcgc
    13141 cgcggcaaga tcgacgcccc ggctcaccgt gcaggatggg gagcgaccga ggtcgtgccg
    13201 tgcgcgggcc gagcgggtaa actgccgccc tgcccgctcg ggagaagcac ttgaaattcg
    13261 aacacctgat cgaggtcaat gatctcgcca acccgctgct gaccccgctc acccgcgaag
    13321 agctgtggtt cggcctgctc tgccgcgccg aggacgcgcg cgccttcctg cccggcctgg
    13381 aggcctgccg catcgtcgag cgcggcgagg gcagcctggt ccgcgatctg cacttcggcc
    13441 acgccgtgat ccgcgaccgg gtgaacttcg acaccttgcg ctggatccgc ttcgaaaccg
    13501 aggccaacgc acagcatgcc ggcggcagcc tcacgatcac catcgaggaa cccgagcccg
    13561 gcgccctctt cctgcgtttc gcctaccaca cctcgctgcc cgaccagggc gaggacgcgc
    13621 gctacgcgga attcgtgcgc tcggcctacc accagtccga cctcgacacc gtgcgcatca
    13681 tccgcatgat cgccgcgtcc ggcgggctcg aacagtaggg ccccaaggac tcccgacggc
    13741 gctgcggcgg ccggcttgcc atggcagcgt gctgccgtgc gcgagtggcc tggccagcga
    13801 acttcccgtg cggtggcgtt gcatgtgcgg gccggacggc tcgcacagcc gtttttcgct
    13861 cccgagcgtc gtctcatctt ccctctcacc aacaggaaac cccttcgtca tgtggttcaa
    13921 gaatctgcag gtctatcgcc ttcccgccaa ctgggacatg agcgccgagc gcctcgagga
    13981 acagctcgcg aagaagccct tccacccctg cggcagccag gacatggaga gtcgcggctg
    14041 gctgtcgccg ctgggcaacg aggtgctggt gcacgccgtg ggcggccagt ggctggtctg
    14101 cctcggcttc gagcaccgcc tgctgccctc cgcggtggtc aagcaggagg ccgacgagcg
    14161 cgccgaggag ctggccgagc aacagggcta caagctcggt cgcaagcagt tgaaggagct
    14221 gcgcgagcag gtgacgcagg aactgatgcc gcgcgccttc acccgccgcc gccgcatgtt
    14281 cgcctggatc gacccgatca atggctggct ggtggtggat gccgccagcc agagcaaggc
    14341 cgaggacatg ctcgagcagc tgcgccacac cctcgacagc ttcccgctga ccttgctgcg
    14401 caccgagcgc tcgccgatgt cggcgatggc cgactggctg gccggtggcg aggcgccgga
    14461 cggcttcacc atcgaccagg actgcgagct gcgctcggtg gccgaggaca aggccgcggt
    14521 gcgctatgcg cgtcacccgc tcgaaggcga cgacatcaag ggccacctgg aagccggcaa
    14581 gctgccgacc cggctggcgc tgaccttcga cgaccgcatc agcttcgtgc tgaccgagaa
    14641 gctagagatc aagcgcctcg atttcctcga catcgtgcgc gatcaggtgg gcgaagccga
    14701 caaggacgac gccgaggcgc tgttcaacgc cgaattcgcg ctcatgaccg gcgaactcgc
    14761 ccacctgctg ccggcggtcg tggccgcgct cggcggcgag cttgcaggcg ccgcggtgga
    14821 aaccggcgtg agcgccgagc tgccgcgtgt gcagaaggaa atggccgccg cgcggccggt
    14881 gtccgcgcag accgtcgccg cggacgatcc gccgttctga gcctcgcagc gcctggcagg
    14941 tttggcacgg gcgcacgggc cgggctgctg catcgcgact tgcgtcgctc ccacggtacg
    15001 gctccagaag agcggtggat tctgtaggag cgacgcaagt cgcgatacag gccgtcgaga
    15061 tggaaatgct gcaccgatgg ggggttcgag ccgcaagcag gggtccgctt cttggtgtac
    15121 acagaattta aagatttaaa agatcataaa gatcatgtac cgcccgattc tgtggacaac
    15181 ccggtttttc atggctgaat cagcgtcttg catcaagcaa aacccggtgc acgatggccg
    15241 cgggaaacga gtacggtttg tggatgaaaa acccgccgtc gccgccttca cgacttatcc
    15301 acaatttccg atccccttcg gtcacagctt tcccactgcc cgaaaaacgc cacacgggaa
    15361 ccgatcctca cccgcgaacc cgttccccgg cgagctgcgc taaagtctgc ccatgaacga
    15421 cacgatgttc ctcgaatggt ggcactggga gatcgccggc atcgcgctgg tgctgctcga
    15481 gcttgccctg ccctccttct tcatcatctg gttcgggctc ggtgcgatgc tcaccggatt
    15541 cgtgctgctc gcgatgccgg acctggcgct gtcgcagcag atcgcggtct ggatcatcgc
    15601 ctcgatggcg atgaccgtgc tgtggttcag cttcgcgaag cgcgcgcgcg tcaggaccct
    15661 gatcggcacc gccgccggcg aggtgatcgg ggaagtgggc ctgctggtga gcgcggtcgc
    15721 gcccttcgag cgcggcaagg tgcgcttcca gcgcccggtg ctcggcgccg acgaatgggc
    15781 ctgtgtggcc gagagcgcga tcgcggccgg cgagcgcgtg aaggtggtga gcgtggaggg
    15841 cagctacgtc aaggtgacga aggcctgagc aacaaggaaa gcaaggaaat gatcatgtcg
    15901 gaaggactcg cgatcgcgat cgcggtgctg gtgttcgtgg tgatcacgat cgccaagggg
    15961 gtgcgtctgg tggcgcaggg cgaggaatgg gtggtcgagc gcctgggcaa gtaccacgcc
    16021 acgctgcgcc ccgggctcaa catcctcatc ccctacctcg accgcgtcgc ctacaagctg
    16081 gtgaccaagg acatcatcct cgacgtgcag gagcaggagg tcatcacccg cgacaatgcg
    16141 gtgatcctca ccaacgcgat cgccttcgtg aaggtcaccg acccggtcaa ggcggtgtat
    16201 ggcgtgaccg acttctccga ggcgatccgc aac
//
LOCUS       DAOQNN010000027        15717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1468_length_15717_cov_10.007726, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000027 DAOQNN010000000
VERSION     DAOQNN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 15717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15717
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1468_length_15717_cov_10.007726"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKV97_03220"
     CDS             complement(<1..323)
                     /locus_tag="PKV97_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB04917.1"
                     /translation="MSGDAMPRGNLYLVPVALGEHTPWAHFLPAQAQAIAAGLRHFVV
                     ETARAARAHLKQLDYPHPLRDTDIRELPAEAADAALDALLAPALAGTDLGLLSDAGCP
                     AVADPG"
     gene            complement(320..895)
                     /locus_tag="PKV97_03225"
     CDS             complement(320..895)
                     /locus_tag="PKV97_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNB04918.1"
                     /translation="MKIVLASTSAYRRMLLERLQLPFETDRPETDESPLPGEAPAATA
                     ERLALEKARAVAARWPGALIIGSDQVAHLGDEVFGKPGTEARAIAQLQRMRGQTVVFH
                     TALALLDTRSGHAQVEGVPTHVRFRELSDDEIRRYVERERPLDCAGSAKSEGLGIALL
                     EAMRGDDPTALIGLPLIALARMLRTAGVHLP"
     gene            1037..1561
                     /locus_tag="PKV97_03230"
     CDS             1037..1561
                     /locus_tag="PKV97_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HNB04919.1"
                     /translation="MSQHGDPSRIISDPFRFAAEARTLEGVTAVAEMLRLSDVLASSG
                     GQVSWRIEGSMVEGVEAREPRLHLMIEGELQLCCQRCLGGMAWPVQVDSVLQPVRSGQ
                     PIPEEELEDDEIDAIELDGELDVIALVEDEILLALPIAPRHQVCDAPRPDGGASKESP
                     FAALASLRGSHSAK"
     gene            1591..1770
                     /gene="rpmF"
                     /locus_tag="PKV97_03235"
     CDS             1591..1770
                     /gene="rpmF"
                     /locus_tag="PKV97_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNB04920.1"
                     /translation="MAVQQNKKSPSKRGMHRAHDFLTGPALAVEPTTGETHLRHHVSP
                     SGFYRGKKVAKGKGE"
     gene            1866..2882
                     /gene="plsX"
                     /locus_tag="PKV97_03240"
     CDS             1866..2882
                     /gene="plsX"
                     /locus_tag="PKV97_03240"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNB04921.1"
                     /translation="MGVTIAIDCMGGDHGPVVTVPAALEFLRSHPQASAILVGREEAI
                     APLLGNATTEFGARIRVRAASEVVAMDDPPAIAMRNKKDSSMRVAVDLVKAGEASAAV
                     SAGNTGALMAISRFVLKTLPGIDRPAIATVLPSMKGHTYVLDLGANVDCSAEHLLQFG
                     VMGAMLVAAIEHKDRPTVGLLNIGEEVIKGNDVVKEAGELLRNSGLNFQGNVEGNDIY
                     EGTVDVVVCDGFVGNVALKTSEGLAQMVGAFLKQEFKRTLFTKLLAVAAMPVLKRFKK
                     RVDHRLYNGAPLLGLRGVVLKSHGSADVLAFGNALSRAAEAASNRLIERISERMASMN
                     EAKA"
     gene            2879..3844
                     /locus_tag="PKV97_03245"
     CDS             2879..3844
                     /locus_tag="PKV97_03245"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNB04922.1"
                     /translation="MIYARIAGTGSYLPGEPVSNDDLVKRGIDTSDEWIVSRSGIRSR
                     HLAAADTGASDLARVAAERAIEAAGCEAEDIDLIIVATSTPDYIFPSTAALVQSKLGI
                     RNGGAAFDVQAVCSGFIYALSVAEKFIRSGSNKRALVIGAEVFSRILDWSDRGTCVLF
                     GDGAGAVVLEASERPGILASALHADGSHHPILCVPGTVASGQVIGDPFLRMDGQAVFK
                     FAVKVLGDVANEVLDAAGVQASSVDWLIPHQANIRIIQATAKRLGVPMERVVTTLDHH
                     GNTSAASIPLALDEAVRAGRLRPGHRIVVEGVGGGFTWGAALIDF"
     gene            3890..4819
                     /gene="fabD"
                     /locus_tag="PKV97_03250"
     CDS             3890..4819
                     /gene="fabD"
                     /locus_tag="PKV97_03250"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNB04923.1"
                     /translation="MAFALVFPGQGSQSVGMMAAYGESAVIRDTFAEASEALGEDLWA
                     MVCDGPAERLALTVNTQPLMLTAGVAAWRAWRAAGGPKPALVAGHSLGEYSALVAAGA
                     LAFKDAVPLVRFRAQAMQEAVPAGEGAMAAVMGLEPDAVHAACAEAAQGQVVEAANLN
                     APGQIVIAGAKAAVERAGEIAKAKGAKRAVLLPVSAPFHCALMRPAAERLAERLAGVA
                     IVKPEIDVINNVDVAVYDDPEKIRDALVRQAYSPVRWIELVQAMGARGMSHVIECGPG
                     KVLAGMTKRIAKEVEGGSAHDAASLEQAIAAVR"
     gene            4823..5572
                     /gene="fabG"
                     /locus_tag="PKV97_03255"
     CDS             4823..5572
                     /gene="fabG"
                     /locus_tag="PKV97_03255"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNB04924.1"
                     /translation="MSFSLEGQVALVTGASRGIGRAVALELGRLGATVVGTATSESGA
                     ADIGKALADAGIQGAGMALDVTDAAACEALVGEVEKRFGAVGILVNNAGITRDNLAMR
                     MKDDEWDAVVDTNLRAVFRMSKLVMRGMMKARHGRIINITSVVASSGNPGQANYAAAK
                     AGVAGMTRALARELGSRNITVNCVAPGFIDTDMTRALPEAARDALLGNIALGRLGRPE
                     EIAGAVAFLASPAAAYVTGTTLHVNGGMYMS"
     gene            5661..5900
                     /gene="acpP"
                     /locus_tag="PKV97_03260"
     CDS             5661..5900
                     /gene="acpP"
                     /locus_tag="PKV97_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNB04925.1"
                     /translation="MENIEQRVKKIVAEQLGVNESEIKTESSFVDDLGADSLDTVELV
                     MALEEEFECEIPDEEAEKITTVQQAIDYVNAHLKK"
     gene            6000..7235
                     /gene="fabF"
                     /locus_tag="PKV97_03265"
     CDS             6000..7235
                     /gene="fabF"
                     /locus_tag="PKV97_03265"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNB04926.1"
                     /translation="MSRRRVVVTGLGVISPVGNTVTEAWSNLIAGKSGIGPITRFDAS
                     NFAARIAGEVKDFDVSAYLSPKEARRMDVFIHYGMAAGIQAIRDSGIEVTEANADRIG
                     INIGSGIGGLPMIEDTHNDFLGGGPRKISPFFIPGTIINMISGNLSIMFGMKGPNLAV
                     VTACTTGLHAIGMGARMIGYGDADVMVCGGAESTVTNLAIGGFGSAKALSTRNDDPAT
                     ASRPWDKGRDGFVLGEGAGVMVLEEYEHARRRGAKIYAEVAGFGMSGDAYHMTAPDTD
                     GPRRSMVNAMKDAGVNVDQVQYLNAHGTSTPLGDKNETDAIKLAFGVEAAKQLVVNST
                     KSMTGHLLGGAGGLESVVTALAVYHQISPPTINIFEQDPECDLDYCANVAREMKIDVA
                     LKNNFGFGGTNGTLVFRRV"
     gene            7258..7755
                     /locus_tag="PKV97_03270"
     CDS             7258..7755
                     /locus_tag="PKV97_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04927.1"
                     /translation="MQFPVELKLRGCSLVKASVWVVHAAAALALFHVPVFTDWEAGDV
                     DRAIAACAWALIFLSLFRGLRAQARLDGCTLWLERDGALELLQEPDGEGGLYRVRERS
                     QVVLPMAAWFTLVPAQISAPGQGAPVARTLFLVPGNLSAGSFRLLRVWLRHRSGRVGP
                     EAAAR"
     gene            complement(7804..9402)
                     /gene="nadB"
                     /locus_tag="PKV97_03275"
     CDS             complement(7804..9402)
                     /gene="nadB"
                     /locus_tag="PKV97_03275"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNB04928.1"
                     /translation="MLKQYDVLILGSGTAGQSMALRLADRLRVALVTKRELADSASNW
                     AQGGIAAVLDNTDSIEAHIQDTFVAGAGLCNPTSTRFVVENGKRAIEWLIDRGVPFTR
                     EADSQLGYHLTREGGHSHRRIIHAADATGAAVQATLGDHVRRHPNIDIYEHHIAVDLI
                     TADKLGQSGAGCLGAYVLDIQGERVLTFAARNTVLATGGTGKVYLYTTNPDVATGDGV
                     AMAWRAGCRVGNMEFIQFHPTCLYHPQAKSFLISEAVRGEGGLLKLPDGTRFMPEHDP
                     REELAPRDIVARAIDFEMKKRGIDCVYLDISHKPADWLRGHFPNIHARCLELGIDITK
                     EPIPVVPAAHYSCGGIMTDLAARTGVPGLYAVGESACTGLHGANRLASNSLLECLVFG
                     EAAAEDILSKAQEPAPVLPAWDESRVTDADEEVVISHNWAELRRFMWDYVGIVRTNKR
                     LRRAQHRIRLLSREIHEFYSNFRVSNDLIELRNLVTTADLIVRCALKRKESRGLHHSR
                     DYPETLPRARDTVLRPPARTPRKR"
     gene            9620..10219
                     /gene="rpoE"
                     /locus_tag="PKV97_03280"
     CDS             9620..10219
                     /gene="rpoE"
                     /locus_tag="PKV97_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HNB04929.1"
                     /translation="MSDRELDQQLVERVQRGDKQAFGLLVSKYQRKLNRLLSRLIRDS
                     AEVEDVAQETFIKAYRALGSFRGDSAFYTWLYRIGINTAKNYLVSQGRRAPTSTSFDS
                     EEAETFEEGDQLRDINTPERLLMTRQIGETVEDAMAALPEELRTAIMLRELEGLSYEE
                     IAGIMECPIGTVRSRIFRAREAIAERLRPLLDTAPDKRW"
     gene            10228..10767
                     /locus_tag="PKV97_03285"
     CDS             10228..10767
                     /locus_tag="PKV97_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HNB04930.1"
                     /translation="MKDKVSALFDGDLDDQGMRAVFDGMRKDASLRKEWDTYCLIGDT
                     LRGDSAGSSDFVSRVMVSLDDEPTLLAPGVPAAGLAQRGLVHRLMPLAASVMGVAAVG
                     LVAASLYRQDAPAPQMAVAASPIQFVGSSNVRPVAAPAADPMREYLFAHQGLSRGGPM
                     PAGVQYVRTVSDTREGGAR"
     gene            10782..11732
                     /locus_tag="PKV97_03290"
     CDS             10782..11732
                     /locus_tag="PKV97_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HNB04931.1"
                     /translation="MLGLCALLAQGPASAEHPTDPLSWLGRISTAGQRLNYVGTFVYQ
                     SGSHVETSRIAHRVDASGEYERLEVLDGSPREIIRRGAEVRCVLPDERTVIIDEAQGR
                     RAFPARLPASPTGLAENYRVRKGELGRVAGLEAQALVLEPKDELRYGHVLWADVQSGL
                     LLKSRTLDQHGAVIEQFSFSELRIGGEVENSLLESRFKTDEGWRMVNARGVSMSVEAA
                     GWVLEPAVPGYALTSAMKRPLGRDRGEAVHMVFSDGLAAISVFIEPIGEGSRAGLGPL
                     SSGAINIFKRAIDGHLVTALGEAPEAAVRRIGEGIRPEAR"
     gene            11729..12199
                     /locus_tag="PKV97_03295"
     CDS             11729..12199
                     /locus_tag="PKV97_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNB04932.1"
                     /translation="MMEARARILRIENGQAWVKLSGKQGGCGRCDEPGGCGSVQISHV
                     FGMPRDEFSIPADARFAAGDAVVIRIPDGAPLRAALVSYGLGTLLLLVGAAFGSLLGG
                     VQHGDAWGLAGGVAGLALAWVVNRMLSRSRNWRNSLSMELSVEENCSRRLRVLQ"
     gene            12214..13659
                     /locus_tag="PKV97_03300"
     CDS             12214..13659
                     /locus_tag="PKV97_03300"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNB04933.1"
                     /translation="MRTGAARALALVLCLLLMLCAPKGFAAGTAERAMLPDFTRLVAT
                     QGAAVVNISATQVAAQPQKQPFRLPELDESDPMFEFFRKFIPRMPEYPGAEPDDKSLG
                     SGFIISADGFILTNAHVVEAAESIVVRLADKREFDATVIGADARSDVALIRIEAKDLP
                     HVVLGDPEALAVGEWVLAIGSPFGFEQSVTAGIVSAKGRSLPDENFVPFIQTDVAINP
                     GNSGGPLFNLRGEVIGINSQIYSRTGGFMGLSFAIPIDVAMDVQQQLREKGRVERGRI
                     GVSIQEITRDLADSFGLPRPAGALVSSVEAGGPAALGGVVQGDVIVRFNQRNVENSAD
                     LPRIVAAARPGSKVEVEIYRDGAPRSLSLTLGEWRDPEEEVEPVAVGLATGATNRLGL
                     ELVAPTAQQRRERGLAHGLLVQRAEKSAARAQIVPGDLVLAIVVEGRQVRLDRIEDFE
                     RVVAALKPGQQVTLLVGRGESASYVSLRADK"
     gene            13671..13931
                     /locus_tag="PKV97_03305"
     CDS             13671..13931
                     /locus_tag="PKV97_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNB04934.1"
                     /translation="MERRFTVMSREWCHLCHVLVDALRPLAEAAGWGIEVIDVDQHPE
                     LEARWGEWVPVLLHGEHELCHYHLDEAAVRAYLGGFPLESRA"
     gene            14010..>15717
                     /gene="lepA"
                     /locus_tag="PKV97_03310"
     CDS             14010..>15717
                     /gene="lepA"
                     /locus_tag="PKV97_03310"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNB04935.1"
                     /translation="MKHIRNFSIIAHIDHGKSTLADRLIHFCGGLSEREMEEQVLDSM
                     DIERERGITIKAQTAALHYKAKDGQVYNLNLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DASQGVEAQTVANCYTALDLDVEVVPVLNKIDLPAADPENARGEIEDVIGVDASEAIL
                     CSAKTGLGVEEVLEAIVARVPPPQGEPDGPLKALIIDSWFDNYVGVVMLVRVVDGELK
                     PKDRILLMSNGLQYPCDQVGVFTPRSVGRDALRAGEVGFVIAGIKEIEAAKVGDTITL
                     ANKPASAPLPGFKEIKPQVFAGLYPVESSEYDQLRDSLEKLRLNDSSLQFEPEVSQAL
                     GFGFRCGFLGLLHMDIVQERLEREFDMDLITTAPTVVYEVVLNDNSIIRIENPAKLPE
                     PGKYQEIREPIITATIFLPQDYVGPVITLCNQKRGNQIDMRYHGRQVQLIYEMPMNEV
                     VMDFFDRLKSVSRGYASLDYDFKEYRTADLVKLDLMVGGEKVDALSVIVHRASAQYRG
                     RELAAKLRGLIPRQMFDVPVQAAIGAHIIARETIKALRKNVLAKCYGGDITRKKKLLE
                     KQK"
BASE COUNT         2490 a         5073 c         5510 g         2644 t
ORIGIN      
        1 ccggggtcgg ccaccgccgg gcagccggcg tccgagagca ggccgaggtc cgtgccggcg
       61 agcgcggggg cgagcagcgc gtcgagcgcc gcgtcggcgg cctcggcggg cagctcgcgg
      121 atgtccgtgt cgcgcagcgg gtggggatag tcgagctgct tgaggtgggc gcgcgccgcg
      181 cgcgcggtct cgacgacgaa gtggcgcagg ccggctgcga tcgcctgtgc ctgcgcaggc
      241 aggaagtgtg cccacggtgt gtgctcgccc agggccacgg ggaccaggta gaggttgccg
      301 cgcggcatgg cgtcgccgct catgggagat gcactccggc cgtgcgcagc atgcgcgcga
      361 gggcgatcag cggcaggccg atcagtgcgg tggggtcgtc gccccgcatc gcttcgagca
      421 gcgcgatgcc gaggccttcg gacttggcgc tgccggcgca gtcgagcggc cgttcgcgct
      481 cgacgtagcg gcggatctcg tcgtcggaga gttcgcggaa gcggacgtgc gtcggcacgc
      541 cctcgacctg ggcgtggccg ctgcgggtgt cgagcagggc gagtgcggtg tggaagacca
      601 cggtctgtcc gcgcatgcgc tgcaactgcg cgatcgcacg cgcctcggtg cccggctttc
      661 cgaagacctc gtcgccgagg tgggcgacct ggtccgagcc gatgatcagc gcgccgggcc
      721 agcgcgcggc gacggcacgg gccttctcca gcgccaggcg ttcggcggtg gcggcgggtg
      781 cctcgccggg cagaggcgat tcgtccgtct ccgggcggtc ggtctcgaag gggagttgca
      841 ggcgctcgag cagcatccgg cggtaggcgg aggtggaggc gaggacgatc ttcatgggtc
      901 cggtgcgaac acgacccgcc ggagaccggt cgagttgttc gcgcgactgt gctggagagg
      961 agggatcggg gtggggcttc ccgaatcgaa tgaagccttt acaggccgat ccgaaaaata
     1021 ttatcatgcg cggcttatgt cgcaacatgg cgatccatcc aggatcatct ccgacccgtt
     1081 tcgatttgcc gcggaggcgc gcacgctgga aggcgtgacc gctgtcgcgg agatgctgcg
     1141 tctgtccgac gtgctcgcgt cgtccggcgg gcaggtgtcg tggcggatcg aggggtcgat
     1201 ggtcgaaggc gtcgaggcgc gtgaaccgcg gctgcacctg atgatcgagg gcgagctgca
     1261 gctttgctgc cagcgctgcc tcggcgggat ggcatggccg gtccaggtgg actcggtgct
     1321 gcagccggtg cgttcgggtc agcccatccc cgaggaagag ctcgaggatg acgagatcga
     1381 tgcgatcgag ctcgatggcg agctcgacgt gatcgccctg gtcgaggacg agatcctgct
     1441 cgccctgccg atcgccccgc gacaccaggt ttgtgacgcg ccacggcccg atggtggtgc
     1501 gtcgaaggaa tcgcctttcg ctgcgctggc gtcgctgcga ggcagtcaca gcgcgaaata
     1561 ggcgcgatat ttgttttagg agctttactc atggccgttc agcagaacaa gaagtccccg
     1621 tccaagcgtg gcatgcaccg tgcccacgac ttcctcaccg gtccggcgct ggccgtcgag
     1681 cccaccaccg gcgagaccca cctgcgccac cacgtgagcc ccagcggttt ctaccgtggc
     1741 aagaaggtcg ccaagggcaa gggcgagtaa tcctgctgcc tgaatcgaga agggcggcgt
     1801 cgggcgcatt gcgccggcgc cgcctttttt ccggctcccg aaacctgtaa ttacgcgaga
     1861 acccgatggg tgtcaccatt gcgatcgact gcatgggtgg cgatcacggc ccggtcgtga
     1921 ccgtgccagc cgcgctggaa ttcctgcgca gccatcccca ggccagcgcg atcctcgtcg
     1981 ggcgggagga ggcgatcgcg cccctgctcg gcaacgcgac gaccgagttc ggcgcgcgca
     2041 tccgcgtgcg cgcggcctcc gaggtggtgg ccatggacga tccgcccgcg atcgccatgc
     2101 gcaacaagaa ggattcgtcg atgcgcgtcg cggtcgacct cgtcaaggcg ggcgaggcga
     2161 gcgctgccgt ctcggcgggc aataccggcg cgctgatggc gatctcgcgc ttcgtgctga
     2221 agaccctgcc cgggatcgat cgccctgcga tcgcgaccgt gctgccgtcg atgaaggggc
     2281 acacgtatgt gctcgacctc ggcgccaacg tcgattgcag cgccgagcat ctgctccagt
     2341 tcggtgtgat gggcgcgatg ctcgtcgctg cgatcgagca taaggaccgt cctacggtcg
     2401 gcctgctcaa catcggtgag gaggtcatca agggcaacga cgtcgtcaag gaagcgggcg
     2461 agctgctgcg taacagcggc ctcaacttcc agggcaacgt cgagggcaac gacatctatg
     2521 aaggcacggt ggatgtggtc gtctgcgacg gcttcgtcgg caacgtcgcg ctcaagacct
     2581 ccgagggtct ggcgcagatg gtcggcgcct tcctgaagca ggagttcaag cgcacgctgt
     2641 tcaccaagct gctggcggtg gcggcgatgc cggtgctcaa gcgcttcaag aagcgcgtcg
     2701 atcatcgtct ttacaatggt gcgccgctgc tcggcctgcg cggcgtggtg ctcaagagtc
     2761 acggctcggc cgacgtgctc gcattcggca atgcgctttc gcgcgctgcc gaagcggcgt
     2821 cgaaccggct catcgagcgc atctccgaac gcatggcgag catgaacgag gcgaaggcat
     2881 gatctatgca cggattgccg gtaccggcag ctacctgccc ggcgaaccgg tcagcaacga
     2941 cgacctggtc aagcgtggca tcgatacctc cgacgagtgg atcgtcagcc gctccggcat
     3001 ccgcagccgc catctcgcgg cggccgacac cggggcgagc gacctcgccc gcgtcgccgc
     3061 cgagcgcgcg atcgaggccg ccggctgcga agcggaggac atcgacctca tcatcgtcgc
     3121 cacctccacg cccgactaca tcttcccgag cacggcggcc ctcgtgcagt ccaagctcgg
     3181 catccgcaac ggcggcgccg ccttcgacgt gcaggcggtg tgcagcggct tcatctatgc
     3241 gctcagcgtg gccgagaagt tcatccgctc gggcagcaac aagcgcgcgc tggtcatcgg
     3301 cgccgaggtg ttctcgcgca tcctcgactg gtccgaccgg ggcacctgcg tgctcttcgg
     3361 cgacggcgca ggcgcggtcg tgctcgaggc ctcggagcgg ccgggcatcc tggcttcggc
     3421 gctgcacgcc gacggcagcc accaccccat cctgtgcgtg ccgggcactg tggcctcggg
     3481 gcaggtgatc ggcgatccct tcctgcgcat ggacggccag gccgtgttca agttcgcggt
     3541 caaggtactc ggcgatgtgg ccaacgaggt gctagatgcc gcgggtgtgc aggcgtccag
     3601 tgtcgactgg ctgatcccgc atcaggccaa catccgcatc atccaggcca ccgccaagcg
     3661 gctcggtgtg ccgatggagc gcgtcgtgac cacgctcgat catcacggca acacctcggc
     3721 ggcgtcgatc ccgctcgcgc tcgacgaggc ggtgcgtgcg gggcggttgc gcccggggca
     3781 tcgcatcgtg gtcgagggcg tcggtggcgg cttcacctgg ggcgcggccc tgatcgattt
     3841 ctgagcttcg tttcccgatt tcacactttc gacacaagcg gagaacagca tggcttttgc
     3901 gctggttttt cccggccagg gttcgcaatc ggtcggcatg atggccgcct acggcgagtc
     3961 cgccgtcatc cgtgacacct tcgcggaggc ctccgaggcc ctcggcgagg atctctgggc
     4021 gatggtctgc gacggccctg ccgagcgcct cgccctcacc gtcaacaccc agccgctgat
     4081 gctgaccgcc ggcgtggcgg cctggcgtgc ctggcgggcc gcgggcgggc cgaaaccggc
     4141 cctggtcgcc ggccacagcc tgggcgagta ctcggcgctg gtcgccgcgg gcgcgctggc
     4201 cttcaaggac gccgtgccgc tggtgcgttt ccgcgcccaa gccatgcagg aggccgtgcc
     4261 ggcgggagag ggcgcgatgg cggcggtgat gggcctggag cccgacgcgg tgcatgcggc
     4321 ctgcgccgag gccgcgcagg gccaggtggt cgaggccgcc aacctcaacg cgccggggca
     4381 gatcgtgatc gcgggcgcca aggcggcggt cgagcgcgcg ggcgagatcg ccaaggccaa
     4441 gggggccaag cgtgccgtgc tgctgccggt ttccgcgccc ttccattgcg cgctgatgcg
     4501 cccggccgcc gagcgcctgg ccgagcgctt ggccggtgtg gcgatcgtca agccggagat
     4561 cgacgtcatc aacaacgtcg acgtcgcggt ttacgatgat cccgagaaga tccgcgacgc
     4621 cctggtgcgg caggcgtatt cgccggtgcg ctggatcgag ctcgtgcagg cgatgggcgc
     4681 ccgcggcatg agccacgtca tcgagtgcgg ccccggcaag gtgctcgccg gcatgaccaa
     4741 gcgcatcgcc aaggaagtcg agggtggctc ggcgcatgac gcggcgagcc tggaacaagc
     4801 cattgcggcg gtgaggtaag acatgagttt ttcgctcgaa ggccaggtcg ccctggtcac
     4861 cggcgcgtcg cgcggcatcg gccgtgcggt ggcgctcgaa ctcggccgcc tgggtgcgac
     4921 ggtggtcggc accgccacct cggaatccgg cgccgccgat atcggcaagg cgctcgccga
     4981 cgccggcatc cagggggccg gcatggcgct ggacgtgacc gacgcggcag cctgcgaggc
     5041 gctggtcggc gaggtcgaga agcgcttcgg tgcggtcggc atcctggtca acaatgccgg
     5101 catcacccgc gacaaccttg ccatgcgcat gaaggacgac gagtgggatg cggtggtgga
     5161 caccaatctg cgcgcggtct tccgcatgag caagctggtc atgcgcggca tgatgaaggc
     5221 ccgccacggc cgcatcatca acatcacctc ggtggtggcc agctccggca acccgggcca
     5281 ggccaactac gccgcggcca aggctggcgt ggcgggcatg acgcgcgcgc tggcgcgcga
     5341 actcggcagc cgcaacatca cggtcaactg cgtcgccccc ggtttcatcg acaccgacat
     5401 gacgcgcgcg ctgcccgaag ccgcgcgcga cgccttgctc ggcaacatcg cgctgggccg
     5461 tctcggccgt ccggaagaaa tcgccggcgc cgtggcgttc ctcgcctccc ccgctgccgc
     5521 ctatgtgacg gggaccaccc tgcacgtcaa tggcggcatg tacatgtcct gatttgcgtc
     5581 gggatagcgg ttccgcggcg ttttggtaga atacgccggc tttttacatt cacctgtcac
     5641 cctcaggaag gagttggttc atggagaaca tcgaacagcg cgtcaagaag atcgtcgctg
     5701 agcaacttgg cgtgaacgaa tcggagatca agaccgagtc ttcgttcgtc gatgatctgg
     5761 gcgcggattc gctggacacc gtggaactgg tcatggcgct ggaagaagaa ttcgagtgcg
     5821 agatcccgga cgaagaggcc gagaagatca ccaccgtcca gcaggcgatc gactacgtca
     5881 acgcccacct caagaagtaa tccccgcgca ttttcccgcg cgtcctgtcc gcgttaccgc
     5941 gcatcgttgc ggcaaccgga caggtttgaa tttctccaac gatttcaacg gagtcgcctg
     6001 tgtcgcgtcg tagagtcgtc gtcaccggtc tgggcgtgat ctcgcctgtc ggcaatactg
     6061 ttaccgaagc ctggtccaac ctgatcgccg gcaagagcgg catcggtccg atcacccgtt
     6121 tcgacgcgag caacttcgct gcccgcatcg ccggcgaggt gaaggacttc gacgtgtccg
     6181 cctacctgtc gcccaaggaa gctcgccgca tggatgtgtt catccactac ggcatggccg
     6241 ctggcatcca ggcgatccgc gactccggca tcgaggtcac cgaggccaac gctgaccgca
     6301 tcggcatcaa tattggttcg gggatcggcg gcctgccgat gatcgaggat acgcacaacg
     6361 acttcctcgg tggcggcccg cgcaagatct cgcccttctt cattccgggc accatcatca
     6421 acatgatctc gggcaacctc tcgatcatgt tcggcatgaa ggggcccaat ctcgcggtgg
     6481 tcaccgcctg cacaaccggc ctgcacgcga tcggcatggg cgcgcgcatg atcggctacg
     6541 gtgatgccga cgtgatggtg tgcggtggcg cggaatccac cgtgaccaac ctcgccattg
     6601 gcggcttcgg ctcggccaag gcgctgtcca cccgcaacga cgatcccgcc accgccagcc
     6661 ggccgtggga caaggggcgt gacggcttcg tgctcggcga gggtgccggc gtgatggtgc
     6721 tcgaggagta cgagcacgcc aggcgccggg gcgcgaagat ctacgccgag gttgccggct
     6781 tcggcatgag cggcgacgcc taccacatga ccgcgcccga caccgacggc ccgcgccgca
     6841 gcatggtcaa tgcgatgaag gacgccggcg tgaacgtcga tcaggtgcag tacctcaacg
     6901 cgcacggtac ctcgaccccg ctcggcgaca agaacgagac ggacgcgatc aagctcgcct
     6961 tcggcgtcga agcggcgaag caactggtgg tgaactccac caagtcgatg accggtcacc
     7021 tgctcggtgg cgccgggggg ctggagtcgg ttgtgaccgc gctggcggtg taccaccaga
     7081 tttcgccgcc gacgatcaac atcttcgagc aggatccgga gtgcgacctc gactactgcg
     7141 ccaacgtcgc ccgcgagatg aagatcgacg tggccctgaa gaacaatttc ggcttcggcg
     7201 gaaccaacgg caccctggtg ttccgccggg tgtgagcctt tcgttctgct ccgcggcatg
     7261 cagttccccg tcgaactgaa gctgcgcggc tgcagtctcg tgaaagcgtc ggtgtgggtc
     7321 gtgcatgcag cggccgcact ggcgcttttt cacgtgcctg tcttcacgga ctgggaggcg
     7381 ggagacgtgg atcgggcgat cgccgcctgc gcctgggcgc tgatcttcct gtcgctgttt
     7441 cgcggcttgc gtgcgcaggc tcgtctggat ggctgcacgc tctggctgga gcgcgacggc
     7501 gcgctcgaac tgttgcagga accggacggt gaaggtgggc tgtatcgggt gcgcgaacgc
     7561 tcgcaggtgg tgttgccgat ggccgcctgg ttcacgctgg tgcccgcgca gatatcggcg
     7621 ccggggcagg gcgccccggt tgctcgaacg ctgttcctcg ttcccggcaa tctatccgcc
     7681 ggcagtttcc gcctcctcag ggtctggctg cgccatcgct cggggcgcgt cgggcccgaa
     7741 gccgctgcgc gctgacgcgg tctcgtccgc ggacggatcg cgccggcgcg acagggctga
     7801 cgctcagcgt ttgcggggcg tgcgcgccgg cggacgaagc acggtgtcgc gtgcgcgtgg
     7861 aagtgtctcg ggatagtcgc gcgagtggtg caggccgcgg ctttccttgc gcttgagcgc
     7921 gcagcgcacg atcaggtccg cagtggtcac cagattgcgc aactcgatca ggtcgttgct
     7981 gacgcggaag ttcgagtaga actcgtggat ttcgcgcgac agcaggcgga tgcggtgctg
     8041 ggcgcggcgc aggcgcttgt tggtgcgcac gatgccgacg tagtcccaca tgaagcggcg
     8101 cagttccgcc cagttgtgcg agatcaccac ctcctcgtcg gcgtcggtga cgcggctctc
     8161 gtcccaggcc ggaagcacgg gtgcaggctc ttgcgccttg gagaggatgt cctcggccgc
     8221 ggcttcgccg aacaccaggc attcgagcag cgagttgctc gcgaggcggt tggcgccgtg
     8281 caggccggtg catgcagatt cgcccacggc gtagagcccg ggaacgccgg tgcgcgcggc
     8341 aaggtcggtc atgatgccgc cgcaggagta gtgcgccgcg ggcacgaccg ggatcggctc
     8401 cttggtgatg tcgatgccca gctccaggca gcgcgcgtgg atattgggga agtggccgcg
     8461 cagccagtcg gccggcttgt ggctgatgtc gaggtagacg cagtcgatgc cgcgtttctt
     8521 catctcgaaa tcgatcgcgc gcgcgacgat gtcgcgcggt gcgagctcct cgcgcgggtc
     8581 gtgctcgggc atgaagcgcg tgccgtcggg cagcttcagc aggcctccct cgccgcgcac
     8641 cgcctcggag atcaggaagg acttcgcctg cgggtggtac aggcaggtgg ggtggaactg
     8701 gatgaattcc atgttcccca cccggcagcc cgctcgccac gccatcgcga cgccgtcgcc
     8761 ggtggcgacg tccgggttgg tcgtgtagag atagaccttg ccggtgccgc cggtggcgag
     8821 cacggtgttg cgcgcggcga aggtcagcac gcgctcgccc tggatgtcga gcacgtaggc
     8881 gcccaggcag cctgcgccgg actgccccag cttgtccgcg gtgatgagat cgaccgcgat
     8941 gtggtgctcg tagatgtcga tgttcgggtg tcggcgcacg tggtcgccga gtgtcgcctg
     9001 caccgccgcg ccggtggcgt ccgcggcgtg gatgatgcgc cggtgggaat ggccgccctc
     9061 gcgggtgagg tggtagccga gctgggagtc ggcctcgcgg gtgaagggta cgccgcggtc
     9121 gatcagccat tcgatcgcgc gcttgccgtt ctcgaccacg aagcgcgtcg acgtggggtt
     9181 gcacaggccg gcgccggcga cgaaggtgtc ctggatatga gcttcgatgc tgtcggtgtt
     9241 gtcgagcacc gcggcgatgc cgccctgcgc ccagttgctc gcgctgtcag ccaactcgcg
     9301 cttggtcacg agcgccacgc gcaggcggtc cgccagacgg agggccatcg actggccggc
     9361 ggtaccgctg ccaagaatga gtacgtcgta ctgtttcagc aagatgtcgc tcgggcagga
     9421 tttgcgggag agggaatata gcatgcggtg acaggccgtc gggcgcgccc ggtcgtccgt
     9481 cctgcccgtc cgcgcgcctg aactatttcc acccgaccct gtcagttcca gtgtcttcga
     9541 tttctgctga gtacgtggcc cgctcgctta taatccggga tcttcgaatc tttgaattcc
     9601 ccggaatcaa caggaaccca tgagcgatcg ggaactcgat cagcaactcg tcgagcgtgt
     9661 tcaacgtggc gacaagcagg cgttcggtct gctggtatcc aagtaccagc gcaagctgaa
     9721 cagactgctg tcgaggctga tccgcgactc ggcagaagtc gaggacgtgg cgcaagagac
     9781 cttcatcaag gcttatcgag cgctgggttc gtttcggggt gacagtgcct tctatacctg
     9841 gctgtaccgc atcggcatca acacggcgaa gaactacctg gtctcccagg ggcgcagggc
     9901 cccgacctcg acgagtttcg attctgaaga ggcggagacc ttcgaggaag gcgatcagtt
     9961 gcgcgacatc aacacgcccg agcggctgct gatgacccgc cagatcggcg aaaccgtgga
    10021 ggacgcgatg gccgcactgc ccgaggaact gaggacggcg atcatgcttc gcgagctcga
    10081 agggctcagt tacgaggaga tcgccggcat catggaatgc ccgattggca cggtgcgctc
    10141 gcggatcttc cgtgcgcgcg aagcgatagc agaacgtctg cggcccctgc tggacacggc
    10201 cccggacaaa cgttggtagg tgcaagcatg aaggacaaag tgtcggcgct gttcgacgga
    10261 gatctcgacg accagggcat gcgtgcggtg ttcgacggca tgcgcaagga tgcttcgctg
    10321 cgcaaggagt gggacaccta ttgcctgatc ggcgacaccc tgcgtggcga ctccgccggt
    10381 tcaagtgatt tcgtttcccg tgtcatggtc agcctggatg acgagccgac gctgctcgcg
    10441 cctggggtcc cggcggcagg cctcgcgcag cgcggcctcg tgcatcgcct gatgccgctg
    10501 gccgcttcgg tgatgggggt ggccgcggtg gggttggtgg ccgcgagtct gtaccggcag
    10561 gacgcgccgg cgccgcagat ggcggtcgcc gcgtcgccga tccagttcgt cggttcctcg
    10621 aatgtgcgcc cggtcgccgc tccggcggcc gatccgatgc gcgagtatct cttcgcccat
    10681 cagggcctca gccgcggcgg cccgatgccg gccggcgtgc agtacgtccg caccgtatcc
    10741 gacacgcgcg agggcggcgc tcgatgaggt ggttccccgc catgctcggt ctctgcgcgc
    10801 tgctcgcgca gggaccggcg tctgccgaac acccgaccga ccccttgtcc tggctcggac
    10861 gcatctcgac cgcggggcaa cgactcaatt acgttggcac cttcgtgtat cagtccggca
    10921 gccatgtcga gacctcgcgg atcgcgcatc gtgtcgatgc cagcggcgag tacgagcgcc
    10981 tcgaggtcct cgatggcagt ccgcgcgaaa tcatccgccg tggcgccgag gtgcgttgcg
    11041 tattgcccga cgagcgcacg gtgatcatcg acgaagcgca gggccgtcgc gccttcccgg
    11101 ctcgcctgcc ggcgtcgccg accgggctgg ccgaaaacta ccgtgtgcgc aagggcgaat
    11161 tgggcagggt ggccgggctt gaggcccagg cgctggtcct cgagcccaag gacgaactgc
    11221 gctacggcca cgtgctgtgg gccgacgtgc agtcgggcct gctgctgaag tcgcgtacgc
    11281 tcgatcagca cggtgctgtc atcgagcaat tcagcttcag cgagctgcgc atcgggggcg
    11341 aggtggagaa cagcctgctc gagtcgcgct tcaagaccga cgaaggctgg cggatggtca
    11401 atgcgcgcgg tgtcagcatg tccgtcgagg ctgccggctg ggtgctcgag ccggcggttc
    11461 cgggttacgc gctcacctcg gccatgaagc gccccctggg gcgggatcgc ggtgaagcgg
    11521 tgcacatggt gttcagcgac gggctggcgg cgatctccgt gttcatcgaa ccgatcggcg
    11581 agggttcgcg agccggcctc ggcccgctct ccagcggcgc gatcaacatt ttcaagcgcg
    11641 cgatcgacgg tcaccttgtc accgcactcg gcgaggcgcc agaggcggcg gtgcgtcgca
    11701 tcggcgaggg cattcggccg gaggcacgat gatggaagcg cgcgcccgta tcctgcggat
    11761 cgagaatggt caggcctggg tcaagctcag tggcaagcag ggcggctgcg gacgctgtga
    11821 cgagccgggt ggttgcggct cggtgcagat ctcccacgtc ttcggcatgc cccgggacga
    11881 attctccatc cccgcggacg cccgcttcgc agctggcgat gcggtggtga tccgcatccc
    11941 ggacggtgcg ccgctgcgcg cggcgctggt cagctacggg ctggggacgc tgctgcttct
    12001 ggtcggcgcc gcgttcggca gcctcttggg cggcgtgcag cacggcgatg cctggggtct
    12061 ggccgggggc gtcgcggggc tcgcgctggc ctgggtcgtc aaccggatgc tgtcgcgcag
    12121 ccgcaactgg cggaacagtc tcagcatgga gctctcggtc gaggagaatt gcagccgccg
    12181 gctgcgcgtc ctgcaatgaa ccccgaagtc cgtatgcgta cgggcgccgc gagggcgctc
    12241 gccttggtgc tctgtctcct gctgatgctc tgcgcgccga agggcttcgc ggccggcacg
    12301 gcagagcggg cgatgctccc cgatttcacc cgcctcgtgg cgacacaggg cgccgccgtg
    12361 gtcaatatca gcgccaccca ggtggctgcg cagccgcaga agcaaccctt ccgtcttccg
    12421 gaactcgacg agtcggatcc gatgttcgag ttcttccgca agttcatccc gcgcatgccc
    12481 gagtacccgg gggcggagcc cgacgacaag tcgctcggct cgggcttcat catcagcgcc
    12541 gacggtttca tcctcaccaa tgcgcatgtc gtggaggccg cagagagcat cgtggtccgc
    12601 ctcgccgaca agcgcgagtt cgacgccacg gtgatcggcg ccgatgcgcg cagcgacgtc
    12661 gcgctgatcc gcatcgaggc caaggacctg cctcatgtcg tgctcggcga ccccgaggcg
    12721 ctcgcggtgg gcgagtgggt gctggcgatc ggttcgccct tcggcttcga gcagtcggtc
    12781 accgccggca tcgtcagcgc caaggggcgc agcctgcccg acgagaactt cgtgcccttc
    12841 atccagaccg acgtcgcgat caatccggga aattcgggcg ggccgctgtt caacttgcgt
    12901 ggcgaggtga tcggcatcaa ttcgcagatc tacagtcgta cgggcgggtt catgggtctg
    12961 tccttcgcga tcccgatcga tgtcgcgatg gacgtgcagc agcaactgcg cgagaagggc
    13021 agggtggagc gcgggcgcat cggggtgtcg atccaggaga tcacccgcga cctcgccgac
    13081 agtttcgggc tgccgcgccc tgcgggtgcg ctggtgagca gtgtggaggc cggtggtccg
    13141 gcggcgctcg gcggcgtcgt ccagggcgac gtgatcgtgc gcttcaacca gcgcaacgtc
    13201 gagaattcgg ccgaccttcc gcgcatcgtc gccgcggcgc gcccgggcag caaggtcgag
    13261 gtcgagatct atcgcgacgg ggcgccgcgt tccctgagct tgacgctggg cgaatggcgc
    13321 gacccggagg aagaggtcga gcccgtggcg gtcggtctgg ccacgggcgc gaccaaccgc
    13381 ctcggcctcg aactcgtcgc gcccacggcg cagcagcggc gcgagcgcgg gcttgcgcac
    13441 ggcctgttgg tgcagcgtgc cgaaaagtcc gccgcgcggg cccagatcgt tcccggcgac
    13501 ctcgtgctgg cgatcgtcgt cgaagggcgc caggtcaggc tcgatcgcat cgaggatttc
    13561 gaacgcgtgg tcgccgcact caagcccggg cagcaggtca ccctgctggt agggcgcggc
    13621 gagagcgcgt cctatgtcag cctgcgcgcc gacaagtgag tccggtcgcg atggaacggc
    13681 gttttaccgt gatgagccgg gaatggtgcc acctgtgcca tgtactcgtg gacgccctgc
    13741 gcccgctcgc cgaagcggcc ggctggggca tcgaggtgat cgacgtcgac cagcatcccg
    13801 agctcgaggc gcgctggggt gaatgggtgc ccgtgctgct gcacggggag cacgaacttt
    13861 gccactatca cctcgacgag gccgcggtac gcgcttatct gggcggtttt ccgctagaat
    13921 cgcgggcctg acgcgctgga ccgaacccgc ccggcgacca accgaatcct ttccgaaacg
    13981 ggtgctgaaa ggcacctttt tcgtttcaaa tgaagcacat ccgaaacttc tccatcattg
    14041 cccacatcga ccacggcaaa tcgaccctgg ccgaccgact catccacttc tgcggcggcc
    14101 tgtccgagcg cgagatggag gagcaggtgc tcgactcgat ggatatcgag cgggagcgcg
    14161 gcatcacgat caaggcgcag accgcggccc tgcactacaa ggccaaggac gggcaggtct
    14221 acaacctcaa cctcatcgat acgccgggac acgtcgactt ctcctacgag gtcagccgct
    14281 cgcttgccgc ctgcgagggc gcgctgctgg tggtcgatgc ctcgcagggc gtcgaggcgc
    14341 agaccgtggc caactgctac accgcgctcg acctcgacgt cgaggtggtg ccggtgctca
    14401 acaagatcga cctgcccgcg gccgaccccg agaacgcgcg cggcgagatc gaggatgtca
    14461 tcggagtcga tgcctccgag gccatcctgt gctcggccaa gaccggcctg ggcgtggagg
    14521 aggtgctcga ggccatcgtc gcccgggtgc cgccgcccca gggcgagccc gacgggccgc
    14581 tgaaggcgct gatcatcgac tcctggttcg acaactacgt cggcgtggtc atgctggtgc
    14641 gcgtcgtcga cggcgaactc aagcccaagg accgcatcct gctgatgtcg aacggcttgc
    14701 agtatccctg cgaccaggtg ggcgtgttca cgccgcgctc ggtgggtcgg gatgcgctgc
    14761 gcgcgggcga ggtgggcttc gtcatcgccg gcatcaagga gatcgaggcg gccaaggtgg
    14821 gcgacacgat cacgctcgcc aacaagccgg cgagtgcgcc gctgcccggc ttcaaggaga
    14881 tcaagccgca ggtcttcgcc ggcctgtatc ctgtggaatc ttccgaatac gaccaactgc
    14941 gcgactcgct cgaaaagttg cgcttgaacg actcctcgct gcagttcgag cccgaggtct
    15001 cgcaggcgct cggcttcggc ttccgctgcg gcttcctcgg cctgctgcac atggacatcg
    15061 tgcaggagcg cctcgagcgc gagttcgaca tggacctcat caccaccgcg ccgacggtgg
    15121 tctacgaggt ggtgctgaac gacaactcga tcatccgcat cgagaatccg gccaagctgc
    15181 cggagcccgg caagtaccag gagatccgcg aaccgatcat caccgcgacc attttcctgc
    15241 cgcaggacta cgtcggcccg gtgatcacgc tgtgcaacca gaagcgcggc aaccagatcg
    15301 acatgcgcta ccacggccgc caggtgcagc tgatctacga gatgccgatg aacgaagtcg
    15361 tgatggactt cttcgacagg ctcaagtcgg tgtcgcgcgg ctacgcctcg ctcgactacg
    15421 atttcaagga gtaccgcacg gccgacctcg tcaagctcga cctcatggtg ggcggcgaga
    15481 aggtggacgc gctgtccgtc atcgtgcacc gcgccagcgc gcagtatcgc ggccgcgagc
    15541 tcgccgccaa gctgcgcggc ctgatcccgc ggcagatgtt cgacgtgccg gtgcaggcgg
    15601 cgatcggtgc gcacatcatc gcgcgcgaga ccatcaaggc cctgcgcaag aacgtgctcg
    15661 ccaagtgcta cggcggcgac atcacgcgca agaagaagct gctcgagaag cagaagg
//
LOCUS       DAOQNN010000028        15566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1493_length_15566_cov_11.770808, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000028 DAOQNN010000000
VERSION     DAOQNN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 15566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15566
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1493_length_15566_cov_11.770808"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..2246
                     /locus_tag="PKV97_03315"
     CDS             261..2246
                     /locus_tag="PKV97_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB04936.1"
                     /translation="MDTRTSASEPGQGGAGPDTFPRWLMHHAKARPQQPAMREKEYGI
                     WQTYTWAQVAENVRAIACGLAQLGFKRGDRLAVVGDNRPRLYWSVAACQCLGGIPVMM
                     YQDAVAQEMAYVLQDAEIKFAVVEDQEQVDKMVEIQPDAPLLAHVIYDDPRGMRHYTQ
                     TLLMGLDELQEMGRIHDRNQPDFLDGEIDKGASDDISIMLYTSGTTGKPKGVCQTHGA
                     FIAAARGAIQFDKLTDKEDILSYLPMAWVGDHLFSFAQATVAGFTINCPESGETVMND
                     LREIGPTYYFAPPRVFENLLTQVMIRMEDAGSLKRKIFHHFMDVARRCGADILDGKPV
                     SGGDRFQYWLGNLLVYGPLKNVLGMSRIRVAYTAGAAIGPDLFRFYRSIGINLKQLYG
                     QTETCAYVCLQPDGEIKLDSVGKPAPFVEVKLADNGEILVKGPMLLKAYYKRPDATAE
                     SINAEGYFMTGDAGFFDADGHLKIIDRAKDVGKMADGTMFAPNYIENKLKFFQHIKEA
                     VTFGAGKEFATAFINIDLEAVGNWAEKKGMAYSGYTDLAQQAAVYELIRDCVEKVNAD
                     LAADPNMSGSQIKRFLILHKELDADDGELTRTRKVRRNFIAEKYGVLIEAMFEGRKTQ
                     FIETQVKYEDGRTGKVSADLRIEEVKTFAPQAGKRAA"
     gene            2264..3082
                     /locus_tag="PKV97_03320"
     CDS             2264..3082
                     /locus_tag="PKV97_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB04937.1"
                     /translation="MTDMNSAATTTPSGRRQGEVILDLQNISLRFGGVKALTDISFNI
                     REHEVRAIIGPNGAGKSSMLNVINGVYHPQEGRIMFRGQQRQKMEPHMAAQQGIARTF
                     QNIALFKGMSVLDNIMTGRNLKMKCNLFQQALWLGPALREEIAHREKVEEIVDFLEIQ
                     HIRKTPVGALPYGLQKRVDLGRALAMEPQILLLDEPMAGMNVEEKQDMCRFILDISDQ
                     FGTTIVLIEHDMGVVMDISDRVVVLDYGKKIGDGTPDEVRNNQDVINAYLGSGH"
     gene            3095..4024
                     /locus_tag="PKV97_03325"
     CDS             3095..4024
                     /locus_tag="PKV97_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB04938.1"
                     /translation="MAFFIETMLGGLMTGMLYSLVALGFVLIFKASGVFNFAQGAMVL
                     VAALAMARFSEWSNAALGGDSLFLANVIGIVGAGAVMFVVAWAVERFVLRKLVNQEGA
                     TLLMVTLGIAYVLEGLGQTFFGSEIYSIDVGMPKDPVIMFEGLFEGGVLINKEDFIAA
                     ALAAVLVAGLTVFFQKTAIGRALRAVADDHQAAQSVGIPLNKTWVIVWCVAGLVALVA
                     GIIWGSKMGVQFSLVTVALRALPVVILGGLTSVPGAIVGGLIIGVGEKLSEVYLGPYV
                     GGGIEIWFAYVMALIVLLFRPQGLFGEKIINRV"
     gene            4040..5116
                     /locus_tag="PKV97_03330"
     CDS             4040..5116
                     /locus_tag="PKV97_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB04939.1"
                     /translation="MFYRENGQFKTSYAADQQIFTIAQDKWFVWGLIAFAALAVPMLA
                     DEYMFRAILIPFLVLSLAALGVNLLVGYCGQITLGAGAFMAIGAYAAYNFMVRIDGMP
                     LVLAMLLGGVSAAAFGILFGIPSLRVRGLYLAVATLAAQFLCDWAFLRIGWFTNHSSS
                     GSVSVAKLEMLGWVIDSPTEKYLFCLAFVIAFAFIAKNLTRAAIGRQWMAIRDMDVAA
                     EVIGIRPMFAKLSAFAVSSFFVGVAGALWGFVHLGSWEPAAFSVDRSFQLLFMVIIGG
                     MGSIMGSFFGAAFIVILPIFLNQFLPLLGSLVGVTVSTAGISHAELIIFGSLIIFFLI
                     VEPHGLARLWSIAKQKLRLWPFPH"
     gene            5168..6499
                     /locus_tag="PKV97_03335"
     CDS             5168..6499
                     /locus_tag="PKV97_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB04940.1"
                     /translation="MKFRNLALAAAIASVGLSSAVFSTAAQAQAQDQFFPVLVYRTGA
                     YAANGNPWANGYVDYLKLINAQGGINGVKLTYEECEFGYATDRGVECYERLKGKNATL
                     FQSLSTGVTFALTDKATVDKIPLITAGYGRSESQDGSVFTWNFPLLGSYWVGADVAIQ
                     HIGKQEGGLDKLKGKKIALVYHDSPFGKEPIPLLQERAKMHGFELQLLPVAHPGVEQK
                     ATWLQIRRDRPDHVLLWGWGVMNSTAIKEAQATGYPREKMLGIWWAGAEPDVKDVGAG
                     AKGYSALTLQHGAEPNSKVVKDMLAMVHDKGQGTGPKEEVGSVLYMRGLISAMLGVEG
                     VRQAQERYGKGKVMTGEQVRWGLENLNLDQGKLDAMGFAGVMRPVQTSCTDHMGSSWV
                     RVHTWDGSKWAFSSDWYQADDKVLRPMVLEAASKYAAEKNLQRRTAEQCAQ"
     gene            6565..7380
                     /locus_tag="PKV97_03340"
     CDS             6565..7380
                     /locus_tag="PKV97_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB04941.1"
                     /translation="MNAPNPAFLSVNNIEVIYNHVILVLKGVSLSVQEGSIVALLGGN
                     GAGKTTTLRAVSSLLKGERGEVTKGSVDYNGERVDALTPADLVKRGVVQVMEGRHCFG
                     HLTIEENLRTGAYTRSDGKAAVEETLEKVYNYFPRLKTRRNSQAAYTSGGEQQMCAIG
                     RALMAKPKLVLLDEPSMGLAPLIVEEVFEIVKDLNTKERVSFLLAEQNTMVALRYADF
                     GYIMENGRIVMEGAAKDLASNEDVKEFYLGLAGEGRKSFRDIKHYRRRKRWLS"
     gene            7434..8669
                     /locus_tag="PKV97_03345"
     CDS             7434..8669
                     /locus_tag="PKV97_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB04942.1"
                     /translation="MNFHDTRETRDPEARERELLSRLPAQIAHARAAAPAFGRLLADV
                     DPDSVTSREALATLPVTRKSELLELQKAARPFGGFSAVGWGAACRRVFASPGPLYEPE
                     GARPDYYRLARALFAAGFRAGDLVHNTFSYHFTPAGSMMETAAHALGCTVFPAGVGQT
                     EQQLAAIADLQPNAYVGTPSFLRILLDKADELGVQVSFAKAFVSGEAFPPSMREVFAA
                     RGIAAYQAYATADIGLIAYETPARQGMVADEDILLEIVRPGTGDPVAPGEVGEVVVTT
                     FNPDYPLIRFGTGDLSAVLPGASPCGRTNLRIKGWMGRADQTTKVKGMFVHPGQIAQV
                     VRRHPEITRARLVVDNPELNDRMTLMCEVAGGGSEALAGAVAASIRELTKLRGEVAFL
                     PTGALANDGKVIDDVRAYE"
     gene            8747..9937
                     /locus_tag="PKV97_03350"
     CDS             8747..9937
                     /locus_tag="PKV97_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNB04943.1"
                     /translation="MNPASNAPARAPLAGVRILDLTRLLPGPLATQHLADYGAEVIKV
                     EDTGAGDYARTMGAMGGDTSWFYQLVNRNKRSLRLDLKQEAGRALFLRLVESADVVVE
                     GFRPGVMERLGLGYAVLAERNPRVVLCSISGYGQTGPYALRAGHDINYLGYAGVLDQI
                     GCAGGAPALSNLQIGDLLGGTMGAVMGILVALFDARRSGRGREVDVSMTDAAFAHMIF
                     PLAEVLAHGGVRPRGEDLLTGGVPCYGVYETADGRYMAVGALEEKFWGLLCTTLGRAD
                     LIPGHLATGADGARVRGELATIFRGRSQREWVEVFDPVDCCVTPVLRLEESLRDPQLR
                     ARGMVVETNGLCHAGAAVRLGGMPPIPDAPAPACAGADTDAILEALGVDAEELARLRG
                     AGVV"
     gene            complement(9953..10384)
                     /locus_tag="PKV97_03355"
     CDS             complement(9953..10384)
                     /locus_tag="PKV97_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04944.1"
                     /translation="MSNDQNAQDPLEFVRGMWNQMGFSLPGMVTPTLDVDELDKRITD
                     MRAVEGWLKMNLNMLQMSIQGLEMQRAALAAVKAMSQQAQQAGEGGGATEPGANPFAA
                     AAAMWPWNLMGAQAAATPAPATPAEAKEGARGTRRKPATDK"
     gene            complement(10425..12326)
                     /locus_tag="PKV97_03360"
     CDS             complement(10425..12326)
                     /locus_tag="PKV97_03360"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNB04945.1"
                     /translation="MIHRWGIRARVTLVAFIPMLVLAGLMTATHTTLRLGELDNALRA
                     RAQAYVRQTAVASEYPLFIGDHIALQQLLDSLLLEEDMAALALIAPDRSLIARAGSFE
                     HTTPPQAAVAGTTRSAGRLRIVEPIRPRPLPIDAVSDPVPADELLATLIIEMSLERLD
                     QRRAELLWIAAGWMLMVAVGSLALARGMSYSVSGPIRNIADTVLRIGRGELDRRVPVV
                     GGGTLRILAEGVNEMAGRLSTAHASMSRQIEEATAELRARRDEAERASIAKSRFLAAA
                     SHDLRQPLHALGLFVSELSQHELDAGSRQILERITAAAETMDGLLGSLLDISRLDSGA
                     LTPVPQSFDLQARLERLVAGQSAVAAERGLDLHLRCAPCRAHTDPLLLDRILANLLSN
                     AIRHTPHGRVLVACRRRGDRLRVEVRDNGPGIAPESQELIFHEFVQLDNPERGQDKGL
                     GLGLAIVRRLSTLLRLPLDLRSRPGHGTVFAVEVPAAQPEGASRAHTPGSFEGVRVQL
                     AGADPAALEATAELLADWGCTVDDGTRTAPADDRPHGTELLILAPVPAGTADGLVLLA
                     ALRMRPDLADVPAILLVVDSTDEQRDRARALNAHLLARPLRPSRLRALMNRLLGARDG
                     DGAQGSGIG"
     gene            12586..13212
                     /locus_tag="PKV97_03365"
     CDS             12586..13212
                     /locus_tag="PKV97_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB04946.1"
                     /translation="MTKILIVEDHALVREAMAQRIGRLEPDLVCLESARADEALALLE
                     AHDDVDLAIVDLMLPGASGFSLLGVLAKRYPDLPAIVVSAMDDDAAVRRALAAGASGF
                     VSKASSGEVLAQAVREVLEGGMPTPRASPSATSARTRRSAERYGVTLAQARVMELLAT
                     GGSNREIASRLGLTEGTVKVHMTAIYRALGVSSRSQALVLLSRRNEES"
     gene            complement(13240..13947)
                     /locus_tag="PKV97_03370"
     CDS             complement(13240..13947)
                     /locus_tag="PKV97_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04947.1"
                     /translation="MAEIICVIGNKGGTGKTTLSHMLCQGLGLLGQRAACVLTDTSRE
                     PLVPAGRRYVLADARTRDSLTKVVDKLRTLENWIGVIDGGGNRTEMDRKLYALSDLVL
                     LPFRESHEDIRTVLRDLEMFPRAYAIPTQWPTNPWQREAADRSVARLLGAHRDRILRP
                     VNALSSTKLLLQKQLPEQLPTPLANACRAVARQLLEVLEIDFIDHNEDTAGEEQDERL
                     SPAEMPMRLVGGARRSH"
     gene            complement(14047..14802)
                     /locus_tag="PKV97_03375"
     CDS             complement(14047..14802)
                     /locus_tag="PKV97_03375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB04948.1"
                     /translation="MPPALKALLAQLAGLAAAVALARTGALVGLWPLVSVQALVAATV
                     AAGLRSARWWLPIHLGFLPLAVVAHGAGIHPGWYLAAFVILVLVYWTSFRTQVPLYLS
                     NPATAAAVGKLLPAHPARMLDIGAGTGALLRPLAQARPDCRFTGIELAPATWAIGRLL
                     LRRHANIEWLRGDLFAADWSGYDLVYAFLSPVPMAAVWAKASAEMPPDSTLVSNSFEV
                     PGHEPDAVIDVGDRRGTRLFLYRMGARGERRSG"
     gene            15018..15102
                     /locus_tag="PKV97_03380"
     tRNA            15018..15102
                     /locus_tag="PKV97_03380"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15052..15054,aa:Tyr,seq:gta)
     gene            15216..15289
                     /locus_tag="PKV97_03385"
     tRNA            15216..15289
                     /locus_tag="PKV97_03385"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15248..15250,aa:Gly,seq:tcc)
     gene            15320..15394
                     /locus_tag="PKV97_03390"
     tRNA            15320..15394
                     /locus_tag="PKV97_03390"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15352..15354,aa:Thr,seq:ggt)
     gene            15479..>15566
                     /locus_tag="PKV97_03395"
     CDS             15479..>15566
                     /locus_tag="PKV97_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNB04949.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLT"
BASE COUNT         2512 a         5083 c         5497 g         2474 t
ORIGIN      
        1 gtggcgtcac gcgcgggatc ggccgacagg ccggcaagtc gcagtacggc ttcgcgttcc
       61 cggtctgagg gccggccgcg gatacgcagg tggcctgcaa caattcagta cattccacaa
      121 acagttgcgc aaaagtggct tgccacactc cagcctgaac tggaacggac gcgttcgcgc
      181 gtcccgcccg ggccctgaca aaaagcacca ggggcccggg tgcggagaca gacaggacaa
      241 gaccaaagga ggagtgtggc atggacacac gcacgagcgc gtccgagccc ggacagggcg
      301 gagcagggcc ggacaccttt ccgcgctggc tgatgcacca cgccaaggcg cgcccccagc
      361 agccggcgat gcgcgagaag gaatacggca tctggcagac ctatacctgg gcgcaagtgg
      421 ccgaaaacgt gcgcgcgatc gcctgcgggc tggcgcagct cggcttcaag cgcggtgacc
      481 gcctcgcggt ggtgggcgac aaccgcccgc ggctgtactg gtcggtggcg gcctgccagt
      541 gcctgggcgg catcccggtg atgatgtacc aggacgcggt ggcccaggag atggcctacg
      601 tgctgcagga cgccgagatc aagttcgccg tggtcgagga ccaggagcag gtggacaaga
      661 tggtcgagat ccagcccgat gcgccgctgc tcgcgcatgt gatctacgac gacccccgcg
      721 gcatgcgcca ctacacccag accctgctga tgggcctgga cgagctgcag gagatggggc
      781 gcatccacga ccgcaaccag cctgacttcc tcgacggcga gatcgacaag ggcgcctccg
      841 acgacatctc gatcatgctg tacacctcgg gcaccaccgg caagcccaag ggcgtgtgcc
      901 agacccacgg cgccttcatc gccgcggcgc gcggcgcgat ccagttcgac aagctcaccg
      961 acaaggagga catcctgtcc tacctgccga tggcctgggt gggcgaccac ctgttctcgt
     1021 tcgcgcaggc cacggtggcg ggcttcacga tcaactgccc ggagtcgggc gagacggtga
     1081 tgaacgatct gcgcgagatc ggacccacct attacttcgc cccgccgcgc gtgttcgaga
     1141 acctgctgac ccaggtgatg atccgcatgg aagacgccgg cagcctcaag cgcaagatct
     1201 tccatcactt catggacgtg gcgcggcgct gcggcgccga catcctggat ggcaagccgg
     1261 tgtcgggtgg cgatcgcttc cagtactggc tgggcaacct gctggtgtat gggccgctga
     1321 agaacgtgct cggcatgagc cgcatccgcg tggcctacac ggccggcgcg gcgatcgggc
     1381 ccgacctgtt ccgcttctac cgctcgatcg gcatcaacct caagcagctc tacggccaga
     1441 ccgagacctg cgcctacgtg tgcctgcagc ccgacggcga gatcaagctg gattcggtcg
     1501 gcaagccggc acccttcgtc gaggtcaagc tcgccgacaa cggcgagatc ctggtcaagg
     1561 ggccgatgct gctcaaggcc tactacaagc gccccgacgc gaccgccgag tcgatcaacg
     1621 ccgagggcta cttcatgacc ggcgacgccg gcttcttcga cgccgacggc cacctgaaga
     1681 tcatcgaccg tgccaaggac gtcggcaaga tggccgacgg cacgatgttc gcgcccaact
     1741 acatcgagaa caagctcaag ttcttccagc acatcaagga ggcggtgacc ttcggtgccg
     1801 gcaaggaatt cgctaccgcc ttcatcaaca tcgacctcga ggcggtgggc aactgggccg
     1861 agaagaaggg catggcctac tccggctaca ccgacctcgc ccagcaggct gcggtgtacg
     1921 agctgatccg cgactgcgtc gagaaggtca acgccgacct cgccgccgac cccaacatga
     1981 gcggctcgca gatcaagcgc ttcctgatcc tgcacaagga gctcgacgcc gacgacggcg
     2041 agctcacgcg cacgcgcaag gtgcggcgca atttcatcgc cgagaagtac ggcgtgctga
     2101 tcgaggcgat gttcgaaggc cgcaagaccc agttcatcga gacccaggtc aagtacgagg
     2161 acggccgcac cggcaaggtc tcggccgacc tgcgcatcga ggaggtgaag accttcgcgc
     2221 cgcaggccgg caagcgcgcg gcctgagggg acgaagaatg gacatgaccg acatgaattc
     2281 cgctgccacc accacgcctt cgggccgtcg ccagggcgag gtcatcctcg acctgcagaa
     2341 catctcgctg cgcttcggcg gtgtaaaggc gctgaccgac atcagcttca acatccgcga
     2401 gcatgaggtg cgcgccatca tcggccccaa cggcgcgggc aagagctcga tgctcaacgt
     2461 gatcaacggc gtgtatcacc cgcaggaagg caggatcatg ttccgcgggc agcagcgcca
     2521 gaagatggag ccgcacatgg cggcccagca gggcatcgcg cgcaccttcc agaacatcgc
     2581 gctgttcaag ggcatgagcg tgctcgacaa catcatgacc gggcgcaatc tcaagatgaa
     2641 gtgcaacctc ttccagcagg cgctgtggct cggcccggcg ctgcgcgagg agatcgccca
     2701 ccgcgagaag gtcgaggaga tcgtcgactt cctcgagatc cagcacatcc gcaagacccc
     2761 ggtgggcgcg ctgccctacg gcctgcagaa gcgcgtcgac ctcggccgcg cgctggcgat
     2821 ggagccgcag atcctgctcc tcgacgagcc gatggcgggc atgaacgtcg aggaaaagca
     2881 ggacatgtgc cgcttcatcc tcgacatcag cgaccagttc ggcaccacga tcgtgctcat
     2941 cgagcacgac atgggcgtgg tgatggacat ctccgaccgc gtcgtggtgc tcgattacgg
     3001 caagaagatc ggcgacggca cacccgacga ggtgcgcaac aaccaggatg tcatcaacgc
     3061 ctatctcggc agcgggcatt gagcggagcg agtcatggca tttttcattg aaacgatgtt
     3121 gggcgggctg atgaccggca tgctgtattc gctggtggcc ctgggcttcg tgctcatctt
     3181 caaggcctcg ggcgtgttca acttcgccca gggcgcgatg gtgctggtcg ccgcgctggc
     3241 gatggcgcgc ttctccgagt ggagcaacgc cgcactgggc ggcgacagcc tcttcctcgc
     3301 caacgtgatc ggcatcgtcg gcgccggcgc ggtgatgttt gtggtggcct gggcggtgga
     3361 gcgcttcgtg ctgcgcaagc tggtgaacca ggagggcgcc acgctgctga tggtcacgct
     3421 gggcatcgcc tacgtgctcg agggcctcgg ccagaccttc ttcggcagcg agatctactc
     3481 gatcgacgtc ggcatgccca aggatccggt gatcatgttc gaaggcctct tcgagggtgg
     3541 cgtgctgatc aacaaggaag acttcatcgc cgcggcgctc gctgccgtgc tggtcgccgg
     3601 gctgacggtg ttcttccaga agaccgcgat cggccgcgcg ctgcgcgcgg tggccgacga
     3661 ccaccaggcc gcgcaatcgg tgggcatccc gctcaacaag acctgggtga tcgtgtggtg
     3721 cgtggccggc ctggtggccc tggtggccgg catcatctgg ggctcgaaga tgggcgtgca
     3781 gttctcgctc gtcaccgtgg cgctgcgcgc gctgccggtg gtgatcctcg gcggcctgac
     3841 ctcggtgccg ggcgcgatcg tcggcggcct catcatcggc gtcggcgaga agctctccga
     3901 ggtctatctc ggaccctacg tcggcggcgg catcgagatc tggttcgcct acgtgatggc
     3961 gttgatcgtg ctgctgttcc ggccgcaggg cctgttcggc gaaaagatca tcaaccgcgt
     4021 ctgaccggga gacaacacag tgttctatcg cgaaaacggc cagttcaaga cgagctacgc
     4081 ggccgaccaa cagatcttca ccatcgccca ggacaagtgg ttcgtctggg ggctgatcgc
     4141 ctttgccgca ctcgccgtgc cgatgctggc cgacgagtac atgttccgcg ccatcctgat
     4201 ccccttcctg gtgctgtcgc tcgccgcgct cggggtcaat ctgctggtgg gctattgtgg
     4261 ccagatcacc ctgggggcgg gggccttcat ggcgatcggg gcatatgcgg cgtacaactt
     4321 catggtgcgc atcgacggca tgccgctggt gctggcgatg ctgctgggcg gcgtgtcggc
     4381 tgcagccttc ggcatcctgt tcggcattcc cagcctgcgc gtgcgcggcc tctacctcgc
     4441 ggtggcgacg ctggcggcgc agtttctgtg cgactgggcc ttcctgcgca tcggctggtt
     4501 caccaaccat tcgtcgtccg gctcggtgtc ggtcgccaag ctggagatgc tgggctgggt
     4561 gatcgactcg ccgaccgaga agtatctgtt ctgcctcgcc ttcgtgatcg cgttcgcgtt
     4621 catcgccaag aacctcaccc gcgccgcgat cggccgccag tggatggcga tccgcgacat
     4681 ggacgtggcg gccgaggtga tcggcatccg cccgatgttc gccaagctgt cggcgttcgc
     4741 ggtgagctcc ttcttcgtcg gcgtggccgg tgcgctgtgg ggcttcgtgc acctgggttc
     4801 gtgggagccg gcggcgttct cggtcgatcg ctccttccag ctgctgttca tggtgatcat
     4861 cggcggcatg ggctcgatca tgggcagctt cttcggcgcc gccttcatcg tgatcctgcc
     4921 catcttcctc aaccagttcc tgcccctgct cggaagcctg gtcggtgtca ccgtgtcgac
     4981 cgccggcatc tcgcatgccg aactcatcat cttcggttcg ctgatcatct tcttcctgat
     5041 cgtcgagccg catggcctcg cccgactgtg gtcgatcgcg aagcagaagc tgcgcctgtg
     5101 gccgttcccg cactgatgca gcgccatttc ccgcagtgcc caagaagcct ataaaaggag
     5161 gagactcatg aaattccgca acctggccct cgcggccgcc atcgcctcgg tgggcctgtc
     5221 gtccgccgtg ttctccaccg ccgcgcaagc tcaggcgcag gatcagttct tcccggtgct
     5281 ggtgtatcgg accggcgcct atgccgccaa cggcaaccca tgggcgaacg gctatgtcga
     5341 ttacctcaag ctgatcaatg cccagggcgg catcaacggc gtcaagctga cctacgagga
     5401 gtgcgagttc ggctacgcca ccgaccgcgg ggtggagtgc tacgagcgct tgaagggcaa
     5461 gaacgccacc ctgttccaga gcctgtcgac cggcgtgacc tttgcgctca ccgacaaggc
     5521 caccgtggac aagatcccgc tgatcaccgc cggctacggc cgcagcgagt cgcaggacgg
     5581 ctcggtgttc acctggaact tcccgctgct cggctcgtac tgggtgggcg ccgacgtcgc
     5641 gatccagcat atcggcaagc aggagggcgg gctggacaag ctcaagggca agaagatcgc
     5701 gctggtctat cacgacagcc ccttcggcaa ggagccgatc ccgctgctgc aggagcgcgc
     5761 gaagatgcat ggcttcgagc tgcagctgct gccggtagcc caccccggcg tggagcagaa
     5821 ggcgacctgg ctgcagatcc gccgcgatcg tcccgaccat gtgctgctgt ggggctgggg
     5881 cgtgatgaac tcgaccgcga tcaaggaggc ccaggccacg ggctacccgc gcgagaagat
     5941 gctcggcatc tggtgggcgg gcgccgagcc ggacgtcaag gacgtcggcg cgggtgccaa
     6001 gggctacagc gccctgacgc ttcagcacgg cgcggagccc aacagcaagg tcgtcaagga
     6061 catgctggcg atggtgcatg acaagggcca gggcaccggc ccgaaggagg aggtgggctc
     6121 ggtgctgtac atgcgcggcc tgatctcggc gatgctgggt gtcgaagggg tgcggcaggc
     6181 gcaggagcgc tacggcaagg gcaaggtcat gaccggcgag caggtgcgct ggggcctgga
     6241 gaacctcaac ctcgaccagg gcaagctcga tgcgatgggt tttgccggcg tgatgcgccc
     6301 ggtgcagacg tcgtgcaccg accacatggg ctcgtcgtgg gtgcgtgtgc atacgtggga
     6361 tggcagcaag tgggcgttca gctccgactg gtatcaggcc gacgacaagg tgctgcggcc
     6421 gatggtgctc gaggcggcct cgaagtacgc cgccgagaag aatctgcagc gccgtaccgc
     6481 ggagcagtgc gcgcagtaaa ttgaaactga gttgaaacgt gcctccgcgc cgcgcggtgc
     6541 ggaggccttt tcctggaatc gaacatgaac gctcccaatc cggccttcct cagcgtcaac
     6601 aacatcgagg tcatctacaa ccacgtcatc ctggtgctga agggtgtgtc gctgtcggtg
     6661 caggagggca gcatcgtcgc cctgctcggt ggcaacggcg ccggcaagac caccacgctg
     6721 cgcgcggtga gcagcctgct caagggcgag cgtggcgagg tgaccaaggg ctcggtggat
     6781 tacaacggcg aacgcgtgga cgcgctgacg ccggcggacc tcgtcaagcg cggtgtcgtc
     6841 caggtcatgg aggggcggca ttgcttcggc cacctcacca tcgaagaaaa cctgcgcacc
     6901 ggcgcctaca cccgcagcga cggcaaggcc gcggtcgaag agacgctgga gaaggtctac
     6961 aactatttcc cgcgcctgaa gacgcgacgg aacagccagg ccgcctacac ctcgggtggc
     7021 gaacagcaga tgtgcgcgat cggtcgtgcg ctgatggcca agcccaagct ggtgctgctc
     7081 gacgagccct cgatgggcct tgcgccgctg atcgtggaag aggtgttcga gatcgtcaag
     7141 gatctcaaca ccaaggaaag ggtgagcttc ctgctcgcag aacagaacac catggtcgcg
     7201 ctgcgctatg ccgacttcgg ctacatcatg gaaaacggcc gcatcgtgat ggaaggtgcc
     7261 gccaaggatc tggccagcaa cgaggacgtc aaggagttct acctcggtct ggcgggcgag
     7321 ggccgcaaga gcttccgcga catcaagcac tatcgccgcc gcaagcgctg gctgagctga
     7381 gcgccaccgc cgccgcgaca tcacccgaca atgcatcgag caggggagcc gagatgaact
     7441 tccacgacac gcgcgagacc cgcgatcccg aggcgcgcga gcgcgagctg ctgagccgcc
     7501 tgccggcgca gatcgcccat gcgcgcgccg ccgcgcccgc cttcggccgc ctgctggccg
     7561 acgtcgaccc ggattcggtc accagtcgcg aggcgctcgc cacgctgccg gtcacacgca
     7621 agtcggaact gctcgagctg cagaaggcgg cgcgcccctt cggcggcttt tcggcggtgg
     7681 gctggggtgc ggcgtgcagg cgggtgttcg cctcgcccgg cccgctctac gaacccgaag
     7741 gcgcacggcc cgactactac cgcctggcgc gcgcgctgtt cgccgcgggc ttccgtgccg
     7801 gcgacctggt gcacaacacc ttctcgtacc acttcacgcc cgccggctcg atgatggaga
     7861 cggccgcgca cgcgctcggc tgcaccgtct ttcccgccgg cgtcggccag accgagcagc
     7921 agctcgcggc gatcgccgac ctgcagccca acgcctacgt gggcacgccg tccttcctgc
     7981 gcatcctgct cgacaaggcg gacgagctcg gcgtgcaggt gtcgttcgcc aaggccttcg
     8041 tctccggcga ggccttcccg cccagcatgc gcgaggtctt cgccgcgcgt ggcatcgcgg
     8101 cctaccaagc ctacgccacc gccgacatcg gcctgatcgc ctacgagacc cctgcgcgcc
     8161 agggcatggt ggcggacgag gacatcctgc tcgagatcgt tcgcccgggc accggcgacc
     8221 cggtagcgcc gggcgaggtg ggcgaggtgg tggtgaccac cttcaacccg gactatccgc
     8281 tgatccgctt cggcaccggc gacctctccg cggtattgcc cggcgcctcg ccctgcgggc
     8341 gcaccaacct gcgcatcaag ggctggatgg gacgcgccga ccagaccacc aaggtcaagg
     8401 gcatgttcgt gcatccggga cagatcgcgc aggtggtgcg ccgccacccc gagatcaccc
     8461 gcgcgcgcct ggtggtggac aaccccgagc tcaacgatcg catgacgctg atgtgcgagg
     8521 tcgccggcgg tggcagcgag gcactcgccg gagcggtcgc ggcgagcatc cgcgagctga
     8581 ccaagctgcg cggcgaggtg gcctttcttc ccaccggcgc cctcgccaac gacggcaagg
     8641 tcatcgacga cgtgcgcgcg tacgagtagc cggaggggct ccccggcctc tccctgcggc
     8701 gcgcgctggc atactgccgt cttcccgcaa aacgatggac gcgcacatga accccgcatc
     8761 gaacgctccc gcacgcgcgc cgctcgccgg cgtgcgcatc ctcgacctca cccggctgct
     8821 gcccgggccg ctcgccaccc agcacctggc cgactacggc gccgaggtga tcaaggtgga
     8881 agacaccggt gccggcgact acgcgcgcac gatgggcgcg atggggggcg acaccagctg
     8941 gttctaccaa ctggtgaacc gcaacaagcg cagcctgcgg ctcgacctca agcaggaggc
     9001 ggggcgtgcg ctgttcctgc gcctggtgga aagcgccgat gtggtggtcg agggcttccg
     9061 cccgggggtg atggagcgcc tcggcctcgg ctacgccgtg ctcgccgagc gcaatccgcg
     9121 cgtagtgctt tgcagcatca gcggctacgg acagaccggg ccgtacgcac tgcgcgccgg
     9181 ccacgacatc aattatctcg gctacgccgg cgtgctcgac cagatcggct gcgccggcgg
     9241 cgcgccggcg ctgtcgaacc tgcagatcgg cgacctgctc ggcggcacca tgggcgcggt
     9301 gatgggcatc ttggtggcgc tgttcgacgc gcgccgcagc ggccgcggcc gcgaggtcga
     9361 cgtgtcgatg accgacgccg cgttcgcgca catgatcttc ccgctcgccg aggtgcttgc
     9421 ccacggtggc gtgcggccgc gcggcgagga cctgctcacc ggcggggtgc cctgctatgg
     9481 cgtgtatgag accgcggacg gccgctacat ggcggtgggt gcgctggagg agaagttctg
     9541 ggggctgctg tgcactacgc tcgggcgtgc ggacctgatc ccggggcatc tcgctaccgg
     9601 ggcggacgga gcacgcgtgc ggggcgagct tgcgacgatc ttccgcggac gcagccagcg
     9661 cgagtgggtg gaggtgttcg acccggtcga ttgctgcgtg acgcccgtgt tgcgcctgga
     9721 ggagagcctg cgcgacccgc agctgcgcgc acgcggcatg gtggtggaga cgaacggcct
     9781 gtgccacgcc ggcgcggcgg tgcgtctggg cgggatgccg ccgatacccg acgcgccggc
     9841 accggcgtgt gcgggggcgg atacggatgc gatcctggag gcgctcggcg tggatgccga
     9901 ggagcttgcc cggctgcggg gcgcgggcgt ggtgtagccc gcggcgggtc gattacttgt
     9961 cggtggccgg cttgcggcgg gtgccacgcg cgccttcctt cgcttcggcc ggggtcgcgg
    10021 gagcgggcgt ggcggccgcc tgcgcgccca tcaggttcca cggccacatc gccgccgcgg
    10081 cggcgaaggg attggctcca ggctcggtcg cgccgccgcc ttcgcctgcc tgctgggcct
    10141 gttggctcat cgccttgacc gcggcgagcg cggcgcgctg catctcgagt ccctggatgc
    10201 tcatctgcag catgttcagg ttcatcttga gccagccctc gaccgcgcgc atgtcggtga
    10261 tgcgcttgtc cagctcatcg acatcgagcg tgggcgtgac catgccgggc agggagaaac
    10321 ccatctggtt ccacatgccg cgcacgaact cgagcggatc ctgggcgttc tggtcattgg
    10381 acatcgggct gctccgttcg gggggttgag gtgacggaac aatattaccc gatgccgctg
    10441 ccctgcgctc catcgccgtc gcgcgcgccg agcaggcggt tcatgagggc gcgcaggcgc
    10501 gaggggcgca gcggacgggc gagcagatgg gcattcaggg cgcgggcgcg atcgcgctgc
    10561 tcgtcggtgc tgtcgacgac gagcaggatc gcgggcacgt cggccaggtc ggggcgcatg
    10621 cgcagggctg cgagcaggac aaggccgtcg gccgtgccgg cgggcaccgg cgcgaggatc
    10681 agcagctcgg tgccgtgggg gcggtcgtcc gccggcgccg tgcgggtgcc gtcgtccacc
    10741 gtgcagcccc agtcggcgag cagctcggcg gtcgcctcga gggcggcggg atccgcgccg
    10801 gcaagctgta cccggacccc ttcgaagctg cccggcgtat gcgcccggga ggcgccttcc
    10861 ggctgtgcgg cggggacctc caccgcgaac acggtcccgt ggccgggccg cgagcgcagg
    10921 tcgagcggca gccggagcag ggtgctgagg cggcgcacga tcgccagccc gagcccgagg
    10981 cccttgtcct ggccgcgctc cggattgtcg agctggacga actcgtgaaa gatcagttcc
    11041 tgcgattccg gcgcgatgcc ggggccgttg tcgcgtacct ccacgcgcag ccggtcgccg
    11101 cgacgccggc aggcgaccag cacgcggccg tgcggggtgt ggcggatggc attcgacaac
    11161 agattggcga ggatgcggtc gaggagcagc ggatcggtgt gggcgcgaca gggcgcacag
    11221 cgcaggtgca ggtcgagccc gcgctcggcg gcgacggccg actgaccggc gaccagacgc
    11281 tcgaggcgcg cctgcaggtc gaaggattgc ggcaccgggg tgagcgcacc cgaatcgagt
    11341 cgcgagatgt ccagcaggct gccgagcagg ccgtccatgg tctcggccgc ggccgtgatg
    11401 cgctcgagga tctgccggct gcccgcgtcg agctcgtgct gggagagctc ggacacgaac
    11461 agcccgagcg catgcagcgg ctggcgcagg tcgtggctgg cggcggcgag gaagcgcgac
    11521 ttggcgatgc tggcgcgttc ggcttcgtcg cgacgtgcgc gcagctcggc ggtcgcctct
    11581 tcgatctggc gcgacatcga tgcgtgcgcg gtgctcaggc gcccggccat ctcgttcacg
    11641 ccctcggcca ggatgcgcag cgtgccaccg ccgaccaccg ggacacggcg gtccagttcg
    11701 ccgcggccga tgcggagcac ggtgtccgcg atgttgcgga tcggaccgct gacgctgtag
    11761 ctcatccccc ttgcgagcgc gagactgccg acggcgacca tcagcatcca gccggcggcg
    11821 atccacaaca gctcggcgcg gcgctggtcg aggcgttcca gggacatctc gatgatgagg
    11881 gtggcgagga gttcgtccgc cggcacggga tcggacacgg cgtcgatcgg cagcgggcgc
    11941 gggcggatcg gctcgacgat gcgcaggcga cctgcgctcc gcgtcgtgcc cgcgacggcc
    12001 gcctgtggcg gcgtggtgtg ctcgaagctg cccgcccggg cgatcaggct gcggtccggt
    12061 gcgatcagcg cgagtgccgc catgtcctcc tcgagcagca ggctgtcgag cagctgctgc
    12121 agggcgatgt ggtcgccgat gaacagcggg tactcgctcg ccaccgcggt ctggcggacg
    12181 taggcctgag cgcgtgcgcg caaggcgttg tcgagttcgc ccaggcgcag cgtggtgtgg
    12241 gtcgccgtca tcaggccggc caggaccagc atcgggatga aggcgaccag cgtcacgcgg
    12301 gcgcggatgc cccaacggtg gatcaaggcc gcctcctcgg cacggcattg cggggttcgg
    12361 cagggcagga aggggcgggc atggcgaggg cctccggtgg ggcggggcga gccgtggacg
    12421 gcaagaccgt tctgcgaggc ttgccgaccg tgggactccg cccggggagt cgattctcct
    12481 gcgtttatcg gcgccggcgt agcgcttgcg ctctcatgga gcgtgaagcc cggcggccgc
    12541 ctcatgctat gttcggtgcg gaaagaggca cgcggagggg agggtatgac gaagatcctg
    12601 atcgtggaag atcatgcgct ggtgcgcgag gcgatggcgc agcgcatcgg gcgcctggag
    12661 cccgacctgg tgtgtctcga gtcggcgcgc gcggacgagg cgctggcctt gctcgaggcg
    12721 catgacgacg tcgatctggc gatcgtcgac ctgatgctgc cgggggcgag cgggttctcg
    12781 ctgctcggcg tgctggcgaa gcgctacccc gacctgccgg cgatcgtggt gtcggcgatg
    12841 gacgacgacg ccgcggtgcg ccgcgccctc gcggccggcg cctctggctt cgtctccaag
    12901 gcgagttccg gcgaggtgct ggcccaggcc gtgcgcgaag tcctggaggg cggcatgccg
    12961 acgccgcgtg ccagcccgtc cgcgacgagc gcccgcacgc gtcgctccgc cgagcgctac
    13021 ggggtgaccc tcgcccaggc cagggtgatg gaactcctcg ccacaggggg cagcaatcgc
    13081 gagattgcca gtcggctggg cctcaccgag gggacggtga aggtgcacat gacggcgatc
    13141 taccgcgcgc tgggggtgag cagccgcagc caggcgctgg tgctgctctc gcgcaggaac
    13201 gaggagtcct gagagaatcg tcgtgccgcg attcgcggct cagtgcgatc gtcgagcgcc
    13261 gccgaccagg cgcatcggca tctcggcggg cgacaggcgc tcatcctgct cctcgccggc
    13321 ggtgtcctcg ttgtggtcga tgaagtcgat ctccagcact tccagcagtt ggcgcgcgac
    13381 cgcgcggcag gcgttggcga gcggggtcgg cagctgctcg ggcagttgct tctgcagcag
    13441 cagcttggtg gaggagagtg cgttgaccgg gcgcaggatg cgatcgcggt gcgcgccgag
    13501 caggcgcgcc acgctgcggt cggcggcctc gcgctgccag gggttggtgg gccactgcgt
    13561 ggggatcgcg taggcgcgcg ggaacatctc gaggtcgcgc agcacggtgc ggatgtcttc
    13621 gtgcgactcg cggaagggca gcagcacgag gtcggagagc gcgtagagct tgcggtccat
    13681 ctcggtgcgg ttgccgccgc cgtcgatgac cccgatccag ttctccagcg tgcgcagctt
    13741 gtcgaccacc ttggtcagcg agtcgcgtgt gcgcgcgtcg gccagcacgt agcgccgtcc
    13801 ggcgggtacc agcggctcgc gcgacgtgtc ggtgagcacg caggcagcac gctggccaag
    13861 caggccgagg ccctggcaca gcatgtgcga gagggtggtc ttgccggtgc cgcccttgtt
    13921 gccgatcacg cagatgatct cagccatgac gcttcagtcc tttgcatgca gtccttgcat
    13981 gacggggatt atcgacgccg cacgcaagcg ggcgagcgcg gaaaagcgcc gcttcgcggg
    14041 gccaattcag ccgcttcggc gctctccgcg tgcgcccatg cgatagagga agaggcgcgt
    14101 gccacggcgg tcgccgacgt cgatcaccgc gtccggctcg tggccgggca cctcgaagct
    14161 gttgctgacc agggtgctgt ccgggggcat ctcggcggac gccttggccc agaccgccgc
    14221 catcggcacc ggcgagagga aggcgtacac cagatcgtag ccgctccagt cggcggcgaa
    14281 gaggtcgccg cgcagccact cgatgttggc gtgacggcgc agcagcaggc gtccgatcgc
    14341 ccacgtcgcc ggcgccagct cgatgccggt gaagcggcag tcgggacgcg cctgcgcgag
    14401 cgggcgcagc agcgcaccgg tgccggcgcc gatgtcgagc atccgcgccg gatgggccgg
    14461 cagcagcttc cctaccgccg ctgcggtggc cgggttgctg aggtagagcg ggacctgggt
    14521 gcggaagctc gtccaataca ccagtacgag gatgacgaag gcggcgagat accacccggg
    14581 atggatgccc gcgccgtgcg cgaccaccgc cagcggcaag aagccgagat ggatcggcag
    14641 ccaccagcgc gcgctgcgca agccggccgc gaccgtcgcc gcgacgagcg cctgcacgct
    14701 caccagcggc cacagtccga cgagcgcgcc ggtgcgcgcg agcgcgaccg cggcggccag
    14761 accggcgagc tgggcgagga gggccttcag ggcggggggc atggcgtgtc tgccgaaagg
    14821 atccgaaccg gggggatcat accgccccag ttcgatgatc cgtcgccgag ccgcgtgggt
    14881 tgtcgacgcc ggattcgcgc cagccgcgcc tccggcgaag cgcaggcggc gcagctcgca
    14941 catcgaggct cgaaagtttt tttcaaaagg ctttgacttc tccggggaag gcctctatag
    15001 tgcggggctt cccggacgga gggataccca agcggtcaac gggatcagac tgtaaatctg
    15061 acggctcagc cttcgaaggt tcgaatcctt ctccctccac caagtttcag tcgtgccgtg
    15121 ctcgtcacgg tcggcgtggc aaatgcagtg tatggctgcg agttgtgata ggcgcggagt
    15181 tgcgcgcagg caagagaaca cagttgcagg cgtgtgcggg tgtagctcaa tggtagagca
    15241 gaagccttcc aagcttatga cgagggttcg attcccttca cccgctccag gttcgctgcg
    15301 gtttgcccga ggtttcgggg cccatgtagc tcagtggtag agcactccct tggtaaggga
    15361 gaggccacgt gttcaatcca cgtcatgggc accatccatt ttctgcctgg cctgggcggc
    15421 ggccaacgag tgtggtcgcc gcgccggttt ttatggttct gattttaagg atagcgatat
    15481 ggctaagggt aagttcgagc gcaccaagcc tcacgtcaac gtcggcacca tcggccacgt
    15541 cgaccatggc aagaccacgc tgaccg
//
LOCUS       DAOQNN010000029        15462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1514_length_15462_cov_10.590121, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000029 DAOQNN010000000
VERSION     DAOQNN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 15462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15462
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1514_length_15462_cov_10.590121"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /gene="nth"
                     /locus_tag="PKV97_03400"
     CDS             complement(<1..541)
                     /gene="nth"
                     /locus_tag="PKV97_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNB04950.1"
                     /translation="MKREAIREFFRRLHEADPHPQTELEYASPYQLLVAVVLSAQATD
                     RSVNLATRKLFALAPTPEAMVSLGEEGIADCIKSIGLFRNKAKNTLALSRLLLERHGG
                     EVPAVREALEALPGVGRKTANVVLNTVFRQPAMAVDTHIFRLANRTGLAPGKDVLEVE
                     KALLRRVPKDYLLDAHHWLI"
     gene            complement(559..1278)
                     /locus_tag="PKV97_03405"
     CDS             complement(559..1278)
                     /locus_tag="PKV97_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNB04951.1"
                     /translation="MSPQQFREIAWNGLWKQNTGLAQLLGLCPILAISTSMVNAVSLG
                     LATILVMAMSNLAVSALRNFIPYEIRIPVFVLIIAALVTVVDLSFNAFFHDLYLVLGI
                     FIPLIVTNCIVLARVEAYAAKNDPLTSTVDGIMMGIGLVWVLAVLGGMRELLGAGTLF
                     SGIEMILPGASAWNVFGDGYPGFLIAILPPGAFFALGVLIAAFNAINVRLAARARRNP
                     PPAPTQTAEPKEDAAEPAAAG"
     gene            complement(1281..1949)
                     /locus_tag="PKV97_03410"
     CDS             complement(1281..1949)
                     /locus_tag="PKV97_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNB04952.1"
                     /translation="MSARYSATRTALRTGAIMVVFTLVFTALMAATHRVTQPAIEASA
                     QDAQRRLIDEVLPPGSYDNDLLADVVHFGPAPQIGMDEGGRVWRARKGGEPVALIAEG
                     AATDGYSGRIALLVAVNTNGLLSGVRVTQHKETPGLGDYIDPKKDRRKDMPWIGQFAI
                     ATWKDVNTAAWTVRKDGGTFGYRTGATISARAVVRAAGRSTAFVVERMDALFAAPFGG
                     VLGE"
     gene            complement(1946..2962)
                     /locus_tag="PKV97_03415"
     CDS             complement(1946..2962)
                     /locus_tag="PKV97_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNB04953.1"
                     /translation="MIHSPYVRKAVSVQRTMGLVLLALIPGIVAHALLIGPGVLVNLA
                     ISTITAVGAEALMLHLRKRPVAIAVGDLSAVVTAWLVTLCFPPIVPWWLTVCGALVAI
                     VAVKHLYGGLGQNPFNPAMIAYCSMIVAFPALMSQWPPAGQLSFDTQLALILGGARDI
                     DGITGATALDALRTGLRSGTQTVDAVLQGSAFGALGGRGWEWISVGYVAGGLFLLAKR
                     IITWHMPLAFIGVLAGISTLFWLLDPGRFASPLFHLASGGAMLAAFFIVTDPVSGATT
                     PRGKLLFAGGIALIAWLIRNFGAYPEGIAFGVLLMNMCVPLLDMKTQPPVFGHHKNAK
                     GGGK"
     gene            complement(2970..4691)
                     /gene="rsxC"
                     /locus_tag="PKV97_03420"
     CDS             complement(2970..4691)
                     /gene="rsxC"
                     /locus_tag="PKV97_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNB04954.1"
                     /translation="MLRELFRFHGGVKPATNKSASTTSPIAKAPIPARLVVPLHQSLG
                     SPAIALVEPGQRVLKGERIGAAGGRLSAAIHAPTSGVVRAVEPALVPHASGLREPAVI
                     IESDGEDRWAEPEHINYRITPPDEVRDFVRDAGVVGLGGAVFPTHQKMRPGSGGKTRT
                     LVLNGAECEPFITCDDMLMRERADGIVSGAAILRHMLQAEEVLIGIEDNKPEAIAAMR
                     QAVAAAGEHMEVVAVPTRYPAGGAKQLIRVLTGIEVPHGERSTDYGVQCFNVGTAYSV
                     HEALELGRPLVSRIVTVAGNVERPGNYEVLIGTPMDELVRLCGPRTDTRFLVMGGPMM
                     GVRMPAFDVPVVKATNCVLAADDKLFPPAPPEQPCIRCTECAKACPVELQPFELYWFS
                     RSKNFGKAQEYHLFDCIECGCCAYVCPSHIPLVDYYRFSKGEIWAREREKAAADQARE
                     RFEFRNFRQEREKAEKAAKLAAKAAETRAKLAEAGTAEAPAAAEAPAAEDPKKALIAA
                     ALARAKAQKAEIQPQNTDNLSPQTQAEIADIETRRKALEAAAVPASTAPAPQAADAAD
                     LPDEPRP"
     gene            complement(4691..5242)
                     /gene="rsxB"
                     /locus_tag="PKV97_03425"
     CDS             complement(4691..5242)
                     /gene="rsxB"
                     /locus_tag="PKV97_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HNB04955.1"
                     /translation="MITAILVMAGIAIVLGLTLGYAAIRFKVEGDPLVEKIDAILPQT
                     QCGQCGFPGCKPYAEAIANGEADINQCPPGGEEGVRKLADLLGREFKPLSEEHGVEKP
                     KSVAVIDEQVCIGCTLCIQACPVDAIVGAAKQMHTVVEPLCTGCELCVAPCPVDCIAM
                     VPLAETVHTWKWKYPVVEMRRVA"
     gene            complement(5256..5840)
                     /gene="rsxA"
                     /locus_tag="PKV97_03430"
     CDS             complement(5256..5840)
                     /gene="rsxA"
                     /locus_tag="PKV97_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNB04956.1"
                     /translation="MTSYIFVVIGAVLVNNVVLVRILGLCPFMGVSKKLETAIGMGAA
                     TTFVLTVGAGTSYLIDHYLLQPFDLGYLRTLAFIVVIAAIVQLTELIIQKTSPLLHQV
                     LGIYLPLITTNCAVLGVPLLNVGLKHDLLESLLFGFGSSLGFTLALVLFAGIRERLDG
                     ADVPIAFKGTAIAMITAGFMSLAFMGFAGLDRFH"
     gene            complement(5937..6485)
                     /locus_tag="PKV97_03435"
     CDS             complement(5937..6485)
                     /locus_tag="PKV97_03435"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNB04957.1"
                     /translation="MKYCSNCGATVAFRIPPGDTLPRHVCEQCGTIHYQNPKLVVGAL
                     AEWEDRVLLCRRAIEPRHGMWTLPAGFMENEETTAQAAARETLEEACARIEVGELYTL
                     IDVPHISQVHIIYRARLLDLDFRPGEESLEVALLREDEIPWERIAFRSIAISLRHFFD
                     DRRSGRWQLHTASLAPPPPDWR"
     gene            6630..8558
                     /locus_tag="PKV97_03440"
     CDS             6630..8558
                     /locus_tag="PKV97_03440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB04958.1"
                     /translation="MPQPIDMKRYAQLKAAGDLPSPRGVALAIIRLTQAPDVSLAELG
                     RVIKGDPAFVGRLIKAANGLIAENRRAIVSVQEALMVLGLPAVRTLALGFSLLSTYRK
                     GTCQGFDYPRFWSSALLMAISMQALTQRVRVVAPDEAFSLGLLARVGELSLATLYPEA
                     FSRLLAELKRDPALQQVVLEDQAFAMNHRELGAAMLADWGVPDALVQPVRFHEQPELA
                     GYAEDSREAGIVQCLVLARAVAQLCLAPDSEHVRLMAPMLRLAGRLGLGRGEFVSLCE
                     RVGRDWAEWGKLLQIRTEDAPRFAEVGSAAVDAVGEAPKVAAEAAGPQPMRALVVAGA
                     REDRELLRGELEAAGIVPFTAAGADAALEAALDVQPQLLVADWSEEGERVVRGLRDMR
                     LGRGVYVICMLGSDDEARLLAASGAGVDDFLVRPLRRAALTVRLRACVRTLVLQQELE
                     HEREELRNFAAELSISNRRLQEAALTDPLTGLPNRRRAIEAMEAEWATATRHGRAFSV
                     MIVDLDGFKTINDTHGHDVGDMALRQAAATLRGALRAQDLICRTGGDEFLVLCPDTDL
                     KAAMLVAERLRVAVQASPVETGGPQIFLTASVGVAMRVPAMGSVDALIKCADDSVYRA
                     KQRGRNCIAALQLQSGAG"
     gene            8715..9032
                     /gene="flhD"
                     /locus_tag="PKV97_03445"
     CDS             8715..9032
                     /gene="flhD"
                     /locus_tag="PKV97_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HNB04959.1"
                     /translation="MDRPDFQEEIRELNLAYLMLAQQMLRDDRATALFRLGIGEAFAD
                     LIESMSAAKLVRMASSPTLLPCFRFDDAQLARLMAGEGRDAASAGLHAAILAAGKAAA
                     DAR"
     gene            9043..9630
                     /gene="flhC"
                     /locus_tag="PKV97_03450"
     CDS             9043..9630
                     /gene="flhC"
                     /locus_tag="PKV97_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HNB04960.1"
                     /translation="MKNKSVIEEADDVRRAVEMVQLGARMQMLEVETRLSREKLLRIY
                     KEVRGASPPKGMLPFSTDWFMTWQPNVHSSLFMNLYRYFTGPAGVHGLDAILKSYHLY
                     LDHIETGVIEPALSLTRAWTLVRFFDADLLQMAACTRCGGEFVAHAHDPVHDYVCGLC
                     NMPSRAGSARRKTTTRKAVAPTHKTTAASRKAVVA"
     gene            9823..10677
                     /gene="motA"
                     /locus_tag="PKV97_03455"
     CDS             9823..10677
                     /gene="motA"
                     /locus_tag="PKV97_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HNB04961.1"
                     /translation="MFLIIGYVIILAASLGTYAVHGSLAALWVPMEYLAIIGLMIGGF
                     VAGNGPKAIKGTLGSLSLAVKGSKYNKALYVDLLALLFEILAKVRKEGLMSIENDIEN
                     PDSSPIFSKYPSVAADHHAVEFMTDYLRMMVGGNLNAFEIENLMDMEIETHHQEAHQA
                     PHVVSKVADAVPAFGIVVAVMGVVNVMGSVGQPPAVLGKMIGGALVGTFLGILVSYGF
                     VAPIASQLEQKVEESGKIYQVIKVVLLASMNGYAPQVAVEFGRKVLYSTDRPGFAELE
                     QEIKNRKG"
     gene            10754..11746
                     /gene="motB"
                     /locus_tag="PKV97_03460"
     CDS             10754..11746
                     /gene="motB"
                     /locus_tag="PKV97_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNB04962.1"
                     /translation="MSDDTQQPIVVKRIKKGGGGHHGGAWKIAYADFVTAMMAFFLLM
                     WLLGSTAQGDLEGIADYFQNPLKLAMQGGEGTGDSSSIIKGGGEDLSRKVGQVRRGDT
                     PSTRAINLDAAPARVKSEAEATAQAIAERRERARLIDLKGKLEAVIEANPSLRQFKNQ
                     LLIDIVSEGLRIQLVDAQNRPMFTSASAELRPYTRELLREIAAALNDVDNRVSMSGHT
                     DSAQYAGGERGFSNWELSSNRANAARRELVAGGLEAGKMLRVVGLADTAHLNPGDPLD
                     PANRRISIVVLNRAAEEAMRSQQAPGATPPPGGAQVGRNPADMRGSPGIVPAPN"
     gene            11763..12731
                     /locus_tag="PKV97_03465"
     CDS             11763..12731
                     /locus_tag="PKV97_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNB04963.1"
                     /translation="MSELLKNIDARTRLAGTNKLEILLFTLGVDARSGCRETYGINVF
                     KVREVMRRPTITAAPDMPDAVQGMVSLRGALVPVVDLGRYVGVGDDAAREIMIVTEYN
                     GHTQGFLVEGVDTILRLDWSQMRVPPEMLTARLGGLVTAVTSLADGRLVMMLDVERVL
                     SETSRYDDAFLFRDIAPLPGADAHVVYFADDSSVARKQISQTLDAMGLRHVGAVNGRQ
                     AWEELSRVAARAEANGRKVADFVSLVLTDVEMPEMDGFMLTRQIKADRRFAGIPVIMH
                     SSLSGSSNHQLGLSVGVDEYVSKFEPQRLSQVLTRLLGMAEAASGR"
     gene            12741..13700
                     /locus_tag="PKV97_03470"
     CDS             12741..13700
                     /locus_tag="PKV97_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNB04964.1"
                     /translation="MEHNRHEESALFDAVDGRATLAGSNRMEILLFSLGTGEHFGINV
                     FKVREVSRTPFITRTPNMPRGVEGLISLRGNVIPVLSLGVILGLTSAGAPLGEAMMVT
                     EYSKRTLGFLVDSVDRIVRVDWERVRAPENVSTGTQGFITAITELDDGRLVSILDVES
                     ILADTFGEAVVGDIAPIGNGHEVNVFFVDDSMVARRKITEVLDRLGVRHKHATNGMEA
                     WKRLDSLAAHAENTGHRLIDEIDLIMLDAEMPEMDGYVLTRRIKSDTRFAGIPVVMHS
                     SLSSEANRAMGTRVGVDAYVAKFDAEVLADTIRPMLQRARRTS"
     gene            13731..14129
                     /gene="cheY"
                     /locus_tag="PKV97_03475"
     CDS             13731..14129
                     /gene="cheY"
                     /locus_tag="PKV97_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HNB04965.1"
                     /translation="MSDPKTRFLVVDDFSTMRRIVRNLLKELGFANVDEAEDGAVALQ
                     KLKSSPYDFVVTDWNMPNMDGLTLLQSIRRTPELKHLPVLMITAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLAEKLEKIFEKMGRGRA"
     gene            14161..15015
                     /gene="cheZ"
                     /locus_tag="PKV97_03480"
     CDS             14161..15015
                     /gene="cheZ"
                     /locus_tag="PKV97_03480"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HNB04966.1"
                     /translation="MSKKSKVDSMGDSDELQALFDSIAADPFAAAGAGAEPAASILPV
                     DESGDNDELQALFDAVAEQAGAAAPVAGGTVENAGAAAGEGVVYNRLGQLARQVHDAL
                     RDLGLDTTLHRAAEAVPDARQRLTYVARTTEQAASRVLNATDVAGPIQTELANGADAL
                     CARWDELYAGRLSPEAFRELSADTRAFLGTVGDGARSTGAQLHEIMMAQDFQDLTGQV
                     IKRLVELTRTLETQLLQVLIEAAPAQVLEPEQAAGLLNGPVIDPAGRSDVVQDQAQVD
                     ELLERLGF"
     gene            15091..>15462
                     /locus_tag="PKV97_03485"
     CDS             15091..>15462
                     /locus_tag="PKV97_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNB04967.1"
                     /translation="MSDFAGMEDLLQDFLVEAGDLLSDVDNKLVELEHSPDDPGLLND
                     IFRGFHTIKGGAGFLGAAELVTLCHLTESLFDRLRKHELEVTPERMDVILSATGAVRD
                     MFVDLGRGSRPGPAAPGLLDAL"
BASE COUNT         2423 a         5209 c         5471 g         2359 t
ORIGIN      
        1 ggatcaacca gtggtgggcg tcgagcaggt agtccttggg cacgcgccgc agcagcgcct
       61 tctcgacctc gagcacgtcc ttgccggggg ccagcccggt gcggttggcg aggcggaaga
      121 tgtgggtgtc gaccgccatc gccggctggc ggaagacggt gttgagcacc acgttggcgg
      181 tcttgcggcc gacaccgggc agggcttcga gcgcctcgcg caccgccggc acctcgccgc
      241 cgtggcgttc gagcagcagg cgcgacagcg cgagggtgtt cttcgccttg ttgcggaaca
      301 ggccgatgct cttgatgcag tcggcgatgc cttcctcgcc gagcgagacc atggcctcgg
      361 gcgtgggggc gagcgcgaac agcttgcgcg tggccagatt gacgctgcga tcggtggcct
      421 gggcggacag caccaccgcg accagcaact gatagggcga cgcgtactcg agttcggtct
      481 gcgggtgcgg gtcggcctcg tgcaggcggc ggaaaaactc gcggatcgcc tcgcgcttca
      541 tcgctgccat gcgcgtcctc agcccgcggc ggccggctcg gcagcgtctt ccttcggctc
      601 ggctgtctgt gtcggcgcgg gtggcggatt gcgccgcgcg cgcgccgcca ggcgcacgtt
      661 gatggcgttg aatgcggcga tcagcacgcc gagcgcgaag aaggcgccgg gcggcaggat
      721 ggcgatcagg aaaccggggt agccgtcgcc gaacacgttc cacgccgagg cgccggggag
      781 gatcatctcg atgcccgaga acagcgtgcc ggcgccgagc agctcgcgca tgccgccgag
      841 caccgcgagc acccacacca ggccgatgcc catcatgatg ccgtccaccg tcgaggtgag
      901 cgggtcgttc ttggccgcat aggcctcgac gcgcgcgagc acgatgcagt tggtgacgat
      961 cagcgggatg aagatgccga gcaccaggta gaggtcgtgg aagaaggcgt tgaaggagag
     1021 atccaccacg gtgaccagcg cggcgatgat caggacgaag accgggatgc ggatctcgta
     1081 ggggatgaag ttgcgcagcg ccgacacggc gaggttggac atcgccatca cgaggatggt
     1141 ggccagcccg aggctgaccg cattgaccat gctggtgctg atcgcgagga tcgggcacag
     1201 gccgagcagc tgcgccaggc cggtgttctg cttccacagg ccgttccagg cgatctcgcg
     1261 gaattgttgc gggctcatgc tcattctccc agcacgccgc cgaagggggc ggcgaagagc
     1321 gcgtccatgc gttcgaccac gaacgccgtg ctgcgtccgg ccgcgcgcac cacggcgcgg
     1381 gcgctgatgg tggcgccggt gcggtagccg aaggtgccgc cgtccttgcg cacggtccat
     1441 gcggcggtgt tcacgtcctt ccaggtggcg atcgcgaact ggccgatcca gggcatgtcc
     1501 ttgcggcggt ccttcttcgg gtcgatgtag tcgcccaggc ccggggtttc cttgtgctgg
     1561 gtgacgcgca cgcccgacag caggccgttg gtattgaccg ccaccagcag cgcgatgcgt
     1621 ccggagtagc cgtcggtggc cgcgccctcg gcgatcagcg ccaccggctc gccacccttg
     1681 cgcgcgcgcc acacccgccc gccttcgtcc atgccgatct gtggcgccgg gccgaagtgc
     1741 accacgtcgg cgagcaggtc gttgtcgtag ctgccgggcg gcagcacctc gtcgatcagg
     1801 cggcgctggg catcctgtgc cgaggcctcg atcgcgggct gggtgacacg gtgggtggcg
     1861 gccatcaagg cggtgaacac cagcgtgaac accaccatga tcgccccggt gcgcagggcg
     1921 gtgcgggtcg cggaatagcg tgcgctcatt tgccgcctcc cttcgcgttc ttgtggtggc
     1981 cgaagacggg cggctgggtc ttcatgtcga gcaggggtac gcacatgttc atcagcagca
     2041 ccccgaaggc gatgccctcc gggtaggcgc cgaagttgcg gatcagccag gcgatcaacg
     2101 cgatgccgcc ggcgaagagc agcttgccgc gcggggtggt cgcacccgag accgggtcgg
     2161 tgacgatgaa gaaggccgcc agcatcgcgc cgccgctggc gagatggaac agcggcgagg
     2221 cgaagcggcc cggatcgagc agccagaaca gggtcgagat gccggcgagc acgccgatga
     2281 aggcgagcgg catgtgccag gtgatgatgc gcttggcgag caggaagagg ccgccggcga
     2341 catagccgac cgagatccac tcccagccgc gcccgccgag cgcgccgaag gccgagccct
     2401 gcagcaccgc atcgacggtc tgggttcccg agcgcagccc ggtgcgcagg gcgtcgagcg
     2461 cggtggcgcc ggtgatgccg tcgatgtcgc gcgctccgcc gagaatcagc gcaagctggg
     2521 tgtcgaagga cagctggccg gccggcggcc actgcgacat cagcgccggg aaggcgacga
     2581 tcatcgagca gtaggcgatc atcgccgggt tgaacgggtt ctggccgagg ccgccataga
     2641 ggtgcttgac ggccacgatc gccaccaggg cgccgcacac cgtgagccac cacggcacga
     2701 tcggcgggaa gcacaaggtg accagccagg cggtgacgac ggccgagagg tcgcccaccg
     2761 cgatcgccac cgggcgcttg cgcagatgca gcatcagcgc ctctgcgccc accgccgtga
     2821 tcgtggagat cgccaggttc accagcacgc cggggccgat gagcagtgcg tgggcgacga
     2881 ttccggggat cagcgcgagg agcacgaggc ccatcgtgcg ctgcacgctg accgccttgc
     2941 ggacgtaggg agagtggatc atgcggggct caggggcgag gctcgtcggg gaggtccgcc
     3001 gcgtcggcgg cttgcggcgc gggtgcggtc gaagccggga ctgcggccgc ctcgagcgcc
     3061 ttgcggcggg tctcgatgtc ggcgatctcg gcttgcgtct gcgggctgag gttgtcggtg
     3121 ttctgcggct ggatctcggc cttctgggcc ttggcacggg cgagcgcggc ggcgatcagg
     3181 gccttcttgg gatcttccgc ggcgggggcc tcggccgccg ccggcgcttc ggccgtgccg
     3241 gcctcggcca gcttggcgcg cgtctcggcc gccttggcgg cgagcttggc ggccttctcg
     3301 gccttctcgc gctcctggcg gaagttgcgg aactcgaagc gctcgcgtgc ctggtccgcg
     3361 gccgccttct cgcgctcacg cgcccagatc tcgcccttgg agaagcggta gtagtcgacc
     3421 agcgggatgt gcgaggggca cacgtaggcg cagcagccgc actcgatgca gtcgaagagg
     3481 tggtattcct gtgccttgcc gaaattcttc gagcggctga accagtacag ctcgaagggc
     3541 tgcaactcga ccgggcaggc cttggcgcac tcggtgcagc ggatgcaggg ttgctcgggc
     3601 ggcgccggcg ggaaaagctt gtcgtcggcc gcgagcacgc agttcgtcgc cttcaccacc
     3661 ggcacgtcga aggcgggcat gcgcacgccc atcatcggtc cgcccatgac gaggaagcgg
     3721 gtgtccgtgc gcgggccgca caggcgcacc agctcgtcca tcggcgtgcc gatgaggacc
     3781 tcgtagttgc ccgggcgctc gacgttgccc gcgaccgtga cgatgcgcga caccagcggg
     3841 cggccgagct cgagcgcctc gtggacgctg taggcggtgc cgacgttgaa gcactgcacc
     3901 ccgtagtcgg tggagcgctc gccgtgcggc acctcgatgc cggtgagcac gcggatcagc
     3961 tgcttggcgc ccccggcggg atagcgggtg gggacggcga ccacttccat gtgctcgccc
     4021 gccgccgcga ccgcctgccg catcgccgcg atcgcctcgg gcttgttgtc ctcgatgccg
     4081 atcaggactt cctcggcctg cagcatgtgg cgcaggatcg cggccccgga gacgatgccg
     4141 tcggcgcgct cgcgcatcag catgtcgtcg caggtgatga agggctcgca ctcggcgccg
     4201 ttgagcacca gcgtgcgggt cttgccgccg ctgccggggc gcatcttctg gtgggtgggg
     4261 aacaccgcgc cgccgaggcc gaccacgccg gcatcgcgca cgaagtcgcg cacctcgtcg
     4321 ggcggggtga tgcggtagtt gatgtgttcg ggctcggccc agcggtcctc gccgtcgctc
     4381 tcgatgatca ccgcgggttc gcgcaggccc gaggcgtgcg ggaccagcgc cggctcgacc
     4441 gcacgcacca cgccggaggt gggtgcgtgg atcgcggcgg acaggcgtcc gcccgccgcg
     4501 ccgatgcgtt cccccttgag cacgcgctgg ccgggctcga ccagggcgat ggcggggctg
     4561 cccaggctct ggtgcagggg gacgaccagc cgcgcgggga tgggggcctt agcgatcggc
     4621 gaggtcgtcg aggcgctctt gttggttgcg ggcttcacgc cgccatggaa gcgaaaaagc
     4681 tcacgcagca tcaggcgacc ctccgcatct cgaccaccgg gtatttccat ttccatgtgt
     4741 gcacggtctc ggccagcggc accatcgcga tgcaatcgac cgggcagggc gccacgcaca
     4801 gctcgcagcc ggtgcacagc ggctcgacca cggtgtgcat ctgcttggcc gcgcccacga
     4861 tcgcatccac cggacaggcc tggatgcaca gcgtgcagcc gatgcagacc tgctcgtcga
     4921 tgaccgcgac cgacttcggc ttctcgacac cgtgctcttc ggagagcggc ttgaactcgc
     4981 gtccgagcag gtcggcgagc ttgcgcacgc cctcctcgcc gccgggcgga cactggttga
     5041 tgtcggcctc gccgttggcg atcgcctcgg cgtagggctt gcagccgggg aagccgcact
     5101 ggccgcactg ggtctgcggc aggatggcgt cgatcttctc gaccagcggg tcgccctcga
     5161 ccttgaagcg gatcgcggcg tagcccaggg tgaggccgag cacgatggcg atgccggcca
     5221 tcacgagaat cgcggtgatc atggtggagc gggcctcagt ggaagcggtc gaggccggcg
     5281 aagcccatga acgcgaggct catgaaaccg gcggtgatca tcgcgatcgc ggtgcccttg
     5341 aaggcgatcg gcacgtcggc gccgtccagg cgctcgcgga tgccggcgaa cagcacgagc
     5401 gcgagggtga agccgagcga ggagccgaag ccgaacaaca gggattcgag caggtcgtgc
     5461 ttcaggccga cgttgagcag gggcacgccg agcacggcgc agttggtggt gatcagcggc
     5521 aggtagatgc ccagcacctg gtgcagcagc gggctggtct tctggatgat cagctcggtg
     5581 agttggacga tcgccgcgat cacgacgatg aaggccagcg tgcgcaggta gccgagatcg
     5641 aagggctgca gcaggtagtg gtcgatcagg taactggtgc cggcgccgac ggtgagcacg
     5701 aaggtcgtgg ccgcgcccat gccgatggcg gtctcgagct ttttcgacac gcccatgaac
     5761 gggcacagcc cgaggatgcg gacgagtacg acgttgttca ccaggacggc gccgatgacg
     5821 acgaagatgt agctggtcat gatccgcgcg cggaagggaa ttgcgggatt atcctccgcg
     5881 cccgagtccc gcatcaaccc tgatgcggga gcatgtatcg gccgggcctc cgggcctcag
     5941 cgccaatccg gtggcggcgg ggcgaggctg gcggtgtgca gctgccagcg gccgctgcga
     6001 cggtcgtcga agaagtggcg caggctgatc gcgatcgaac ggaaggcgat ccgctcccag
     6061 gggatctcgt cctcgcgcag cagcgcgacc tcgagcgact cctcgccggg gcggaagtcg
     6121 aggtcgagca ggcgggcgcg gtagatgatg tggacctggc tgatgtgcgg cacgtcgatc
     6181 agcgtgtaca gctcgcccac ctcgatgcgc gcgcaggcct cctccagggt ctcgcgggcg
     6241 gcggcctggg cggtggtttc ctcgttctcc atgaagccgg cgggcagcgt ccacatgccg
     6301 tggcgcggct cgatcgcacg ccggcacagc agcacgcggt cttcccactc ggccagcgcg
     6361 cccaccacca acttggggtt ctggtagtgg atggtgccgc actgctcgca gacgtggcgg
     6421 ggaagggtgt ccccgggcgg gatgcggaag gcgacggtgg cgccgcaatt cgagcagtat
     6481 ttcatggtcg cggacaatgg ttttcggcga ttctagcctg ctttgacccg tctgccggca
     6541 gcacggccta aagaatgacg ccagtcggcc gatgaagcgg gctctgtgcc agaatcggcc
     6601 ggcgacagcg agcgagggag tgtaaaggga tgccgcaacc gatcgacatg aagcgatatg
     6661 cgcagctcaa ggcggcggga gacctgcctt cgccgcgcgg cgtggcgctc gcgatcatcc
     6721 ggctcacgca ggcgcccgac gtctcgctgg ccgagctcgg ccgcgtgatc aagggcgatc
     6781 ccgccttcgt cgggcgcctg atcaaggcgg ccaatggcct catcgcggag aaccggcgcg
     6841 cgatcgtgtc ggtgcaggaa gcgctgatgg tgctcggcct gcccgccgtg cgcacgctgg
     6901 cgctgggctt ctcgctgctg tcgacctacc gcaagggtac ctgccagggc ttcgactacc
     6961 cgcgtttctg gtcctccgcg ctgctgatgg cgatctcgat gcaggcgctc acccagcgcg
     7021 tgcgcgtggt cgcacccgac gaggcgttca gcctcggcct gctcgctcgc gtgggcgagc
     7081 tctcgctcgc gaccctctat cccgaggcct tctcccgcct gctcgcggaa ctcaagcgcg
     7141 atcccgccct gcagcaggtg gtgctcgagg accaggcctt cgcgatgaac caccgcgagc
     7201 tcggtgccgc catgcttgcc gactgggggg tgccggacgc gctggtgcaa ccggtgcgct
     7261 tccacgaaca gcccgaactg gccggctacg cggaggattc gcgcgaggcc gggatcgtgc
     7321 agtgcctcgt gctggcgcgc gcggtggcgc agctgtgcct cgcgcccgat tccgaacacg
     7381 tgcgtctgat ggcgccgatg ctgcgcctgg cgggccgcct cggcctcggg cgcggcgagt
     7441 tcgtctcgct gtgcgagcgc gtcgggcgcg actgggcgga gtggggcaag ctgctgcaga
     7501 tccgcaccga ggacgcgccc cgcttcgccg aggtgggcag cgcggcggtg gatgcggttg
     7561 gcgaggcgcc caaggtggcc gccgaggccg ctggtccgca gccgatgcgt gcgttggtcg
     7621 tggccggcgc gcgcgaggac cgcgagctgc tgcgcggcga gctcgaagcc gcggggatcg
     7681 tcccgttcac cgctgcgggc gcggacgccg cgctcgaggc cgcgctcgac gtccagccgc
     7741 aactgctggt cgcggactgg tcggaggagg gggaacgggt ggtgcgcggc ctgcgcgaca
     7801 tgcgcctcgg acgcggcgtg tacgtgatct gcatgctcgg cagcgacgac gaggcgcgcc
     7861 tgctggccgc ctccggcgcc ggggttgacg acttcctcgt ccgtccgctg cgacgtgcgg
     7921 cgctgaccgt gcgcctgcgt gcctgcgtgc gcacattggt gctgcagcag gaactcgagc
     7981 acgagcgcga ggagctgcgc aatttcgccg ccgagctgtc gatctcgaac cgccgcctgc
     8041 aggaggccgc gctgaccgac ccgctcaccg gtctgcccaa ccgccgtcgg gcgatcgagg
     8101 cgatggaggc cgagtgggcc acggcgacgc gtcacggtcg cgccttctcg gtcatgatcg
     8161 tcgacctcga cggcttcaag accatcaacg acacccatgg gcacgacgtc ggcgacatgg
     8221 ccctgcgcca ggccgcggcg accctgcgtg gcgcgctgcg cgcccaggac ctgatctgcc
     8281 gtaccggtgg cgacgagttc ctggtgctgt gccccgatac cgacctgaag gcggcgatgc
     8341 tggtcgcgga gcgcctgcgc gtggccgtgc aggccagtcc ggtcgagacc ggcggcccgc
     8401 agatcttcct cactgccagc gtcggcgtgg ccatgcgcgt gcccgccatg ggcagcgtcg
     8461 atgcactgat caagtgtgcc gacgacagcg tgtatcgtgc caagcagcgt ggccgcaact
     8521 gcatcgccgc actccagctc cagtccggcg cgggctgaat gccggcggga aggcgcggcc
     8581 gccccgccgg caggtgttgt tcgggggcga caccttgtgc gtgaaatgcg gcacggagcc
     8641 ggggggagcg gcttgccgct cctgagaaac tactttaact tgcagacgtt gatccccgtg
     8701 cgggatggaa aaccatggat cgccccgact tccaggaaga aatccgcgaa ctcaacctcg
     8761 cctacctgat gctggcacag cagatgctgc gcgacgaccg cgcgaccgcg ctgttccggc
     8821 tcgggatcgg cgaggccttc gcggacctga tcgagtcgat gtcggcggcc aagctggtca
     8881 ggatggcgtc cagtccgacg ctgctgccct gcttccgctt cgacgatgcc cagttggccc
     8941 ggctcatggc gggcgaaggg cgggatgccg ccagcgcggg cctgcacgcg gcgatcctcg
     9001 ccgccggcaa ggcggcggcc gatgcgcgct gaggagtgcc gaatgaagaa caagagtgtg
     9061 atcgaagagg ccgacgacgt gcgtcgtgcg gtcgagatgg tgcaactcgg tgcgcgcatg
     9121 cagatgctcg aggtcgagac caggctgagc cgcgagaagc tgctcaggat ctacaaggaa
     9181 gtccgcggtg cctcgccccc caagggcatg ctgccgttct ccaccgactg gttcatgacc
     9241 tggcagccga acgtgcattc ctcgctgttc atgaacctgt accgctactt caccggcccg
     9301 gccggcgtgc acggcctgga cgcgatcctc aagtcctatc acctctatct cgaccacatc
     9361 gagaccggcg tgatcgagcc cgcgctgagc ctcacgcgcg cgtggaccct ggtgcgcttc
     9421 ttcgacgccg acctgctgca gatggccgcg tgtacccgct gcggcggcga gttcgtcgcc
     9481 cacgcccacg atccggtgca cgactacgtg tgcggcctgt gcaacatgcc ctcgcgcgcc
     9541 ggctccgcgc gccgcaagac caccacccgc aaggcggtcg cgcccacgca caagacgacg
     9601 gccgccagcc gcaaggcggt cgtcgcctga ccccggcggg ccctccggcc cgcatgcagg
     9661 ttcctacatg ggccgcgctg gaaacaggcg gcccatgtca cgtccgcgca cggcgtggcg
     9721 tcgcgccccg gcgcgttcaa gttcctcccg ccgccgccgt tatcccctgc gagagccgtg
     9781 ccggtccgtg cgcggcgcag cgaaacgctt cggagtttcc ccgtgttcct gatcatcggc
     9841 tacgtcatca tcctcgccgc ctcgctgggc acctacgcgg tgcatggcag cctggccgcg
     9901 ctgtgggtgc ccatggagta cctcgcgatc atcggcctga tgatcggtgg cttcgtcgcc
     9961 ggcaacggcc cgaaggcgat caagggcacg ctcggctcgc tgtcgctggc ggtgaagggc
    10021 tcgaagtaca acaaggcgct ctatgtcgac ctgctcgcgc tgctgttcga gatcctcgcc
    10081 aaggtgcgca aggaaggcct gatgtcgatc gagaacgaca tcgagaaccc cgactccagc
    10141 ccgatcttct ccaagtaccc gagcgtcgcc gccgaccatc acgcggtcga gttcatgacc
    10201 gactacctgc gcatgatggt gggcggcaac ctcaacgcct tcgagatcga gaacctgatg
    10261 gacatggaga tcgagaccca ccaccaggag gcgcaccagg cgccgcacgt ggtctccaag
    10321 gtcgccgatg cggtgcccgc cttcggcatc gtggtggcgg tgatgggcgt ggtgaacgtg
    10381 atgggctcgg tgggtcagcc gcccgcggtg ctgggcaaga tgatcggcgg cgcgctggtc
    10441 ggcaccttcc tcggcatcct ggtctcgtat ggtttcgtcg cgccgatcgc cagccagctc
    10501 gagcagaagg tcgaggagag cggcaagatc taccaggtca tcaaggtggt gctgctggcg
    10561 agcatgaacg gctatgcgcc gcaggtcgcg gtggagttcg gccgcaaggt gctgtactcg
    10621 accgaccgcc cgggcttcgc cgagctcgag caggaaatca agaaccgcaa gggctgaacg
    10681 cgatgagccc ccgcgtccct gactcccgtc gtccccaggc gccgcgccct gcgccggcgt
    10741 gcggagttgc gcgatgagcg acgataccca gcaaccgatc gtcgtcaagc gcatcaagaa
    10801 gggcggtggc gggcaccacg gcggtgcatg gaagatcgcc tacgcggact tcgtcaccgc
    10861 gatgatggcc ttcttcctgc tgatgtggct gctcggctcg accgcgcagg gcgacctcga
    10921 gggcatcgcc gactacttcc agaacccgct caagctcgcc atgcagggcg gcgaaggcac
    10981 cggcgacagc tcgagcatca tcaagggcgg tggcgaggac ctctcgcgca aggtcggcca
    11041 ggtgcgccgg ggcgataccc ccagcacgcg cgcgatcaat ctcgacgccg cgcccgcgcg
    11101 cgtcaagagc gaggccgagg ccaccgcgca ggcgatcgcc gagcgccgcg aacgcgcgcg
    11161 cctgatcgac ctcaagggca agctcgaggc ggtcatcgag gccaacccct cgctgcgcca
    11221 gttcaagaac cagctcctca tcgacatcgt cagcgagggc ttgcggatac agctggtcga
    11281 cgcgcagaac cggccgatgt tcacctcggc gagcgccgag ttgcgcccct atacgcgcga
    11341 gctgctgcgc gagatcgccg ctgcgctcaa cgatgtcgac aaccgtgtca gcatgtccgg
    11401 ccacacggac tcggcccaat acgcaggcgg cgagcgcggc ttctcgaact gggagctgtc
    11461 ctccaaccgc gccaacgccg cgcgccgcga gctcgtggcg ggcgggctgg aggcgggcaa
    11521 gatgctgcgc gtggtcggcc ttgccgacac cgcgcacctg aatccgggcg atccgctcga
    11581 ccccgcgaac cgacgcatca gcatcgtggt gctcaaccgt gcggccgagg aggcgatgcg
    11641 cagccagcag gcgcccggcg ccacgccgcc gccgggcggt gctcaagtcg ggcgcaatcc
    11701 ggccgatatg aggggaagcc cggggattgt cccggcgccc aattgaatcc aaggctgcag
    11761 ccatgtccga actgctgaag aacatcgacg cccgcacccg gctcgccggc accaacaagc
    11821 tcgagatcct gctgttcacg ctcggcgtgg acgcgcgcag cggctgccgc gagacttacg
    11881 gcatcaacgt cttcaaggtg cgcgaggtga tgcgccggcc gaccatcacc gccgcgcccg
    11941 acatgcccga cgcggtgcag ggcatggtga gcctgcgcgg cgcgctcgtc ccggtggtcg
    12001 atctcggccg ctacgtcggg gtcggtgacg atgctgcgcg cgagatcatg atcgtcaccg
    12061 agtacaacgg ccacacccag ggcttcctgg tcgagggtgt cgacaccatc ctgcgcctgg
    12121 actggtcgca gatgcgggtg ccgcccgaga tgctcaccgc gcgcctgggc gggctggtga
    12181 ccgcggtgac ttcgctcgcc gacggccgcc tggtgatgat gctcgacgtc gagcgcgtgc
    12241 tctccgagac ctcgcgctac gatgacgcct tcctgttccg cgacatcgcg ccgctgccgg
    12301 gtgccgatgc ccacgtggtg tatttcgccg acgactcctc ggtggcgcgc aagcagatct
    12361 cccagaccct ggatgccatg ggcctgcgcc acgtcggcgc ggtcaacggc cgccaggcct
    12421 gggaggaact ctcccgggtc gcggcgcgcg ccgaggccaa tggtcgcaag gtcgccgatt
    12481 tcgtcagcct ggtgctgacc gacgtcgaga tgcccgagat ggatggcttc atgctcaccc
    12541 gccagatcaa ggccgaccgc cgcttcgccg gcatcccggt gatcatgcac tcgtcgctgt
    12601 cgggcagctc gaaccaccag ctcggcctct ctgtcggggt ggatgaatac gtgtccaagt
    12661 tcgaaccgca gcgcctgtcc caggtgctga cccggctgct cggcatggcc gaggccgcga
    12721 gcggacgctg aggaagagcc atggaacaca accgtcacga agaatccgcc ctgttcgacg
    12781 cggtcgatgg ccgtgccacg ctggccggct cgaaccgcat ggagatcctg ctgttctcgc
    12841 tcggtaccgg cgagcatttc ggcatcaacg tcttcaaggt gcgcgaggtc tcgcgcacgc
    12901 ccttcatcac ccgcacaccc aacatgccgc gcggcgtcga gggcctgatc tcgctgcgcg
    12961 gaaacgtcat cccggtgctc tcgctcggcg tcatcctcgg gctgacctct gcgggggcgc
    13021 cgctcggcga ggcgatgatg gtcaccgaat acagcaagcg cacgctcggt ttcctggtcg
    13081 attcggtcga ccgcatcgtg cgcgtggact gggagcgcgt gcgtgcgccc gagaacgtgt
    13141 cgaccggcac gcagggcttc atcaccgcga ttaccgaact cgacgacggc cgcctggtgt
    13201 cgatcctcga tgtcgagtcc atcctcgccg acaccttcgg cgaggcggtg gtcggcgaca
    13261 tcgcgccgat cggcaacggc cacgaggtga atgtgttctt cgtcgacgac tccatggtcg
    13321 cgcgccgcaa gatcaccgag gtgctcgacc gcctcggtgt gcgccacaag cacgcgacca
    13381 acggcatgga ggcgtggaag cggctcgaca gcctggccgc ccacgccgag aacaccgggc
    13441 accggctgat cgacgagatc gacctcatca tgctcgacgc cgagatgccc gagatggacg
    13501 gctacgtgct cacccgccgc atcaagagcg acacgcgctt cgccggcatc ccggtggtca
    13561 tgcactcctc gctgtcgtcc gaggccaacc gcgccatggg cacgcgggtc ggggtggacg
    13621 cctacgtggc aaaattcgac gccgaagtgt tggccgacac catccgcccg atgctgcaac
    13681 gggcgcgtcg gacttcctga ccgaacaggc aaagaacccg gagacatctg atgtccgatc
    13741 ccaagacccg cttcctcgtc gtcgacgatt tctcgaccat gcgccgcatc gtgcgcaacc
    13801 tgttgaagga gctcggcttc gccaacgtcg acgaggccga ggacggcgcg gtggcgctgc
    13861 agaagctcaa gtccagcccc tacgacttcg tcgtcaccga ctggaacatg cccaacatgg
    13921 acggcctgac cctgctgcag agcatccgcc gcacgcccga gctcaagcac ctgccggtgc
    13981 tgatgatcac cgccgaggcc aagaaggaaa acatcatcgc cgccgcgcag gccggtgcca
    14041 gcggctacgt ggtcaagccc ttcaccgcgg cgacgctggc cgagaagctg gagaagattt
    14101 tcgagaagat gggccgcggc cgggcatgaa cctcgtccgc agcgcagacg gagcatggat
    14161 atgagcaaga agtccaaggt ggactcgatg ggcgattcag acgaattgca ggcgctcttc
    14221 gacagcattg ccgccgaccc cttcgccgcg gcgggtgcgg gtgcggagcc ggcggcatcg
    14281 atcctgccgg tcgacgagtc cggggataac gacgagttgc aggcgctctt cgacgccgtc
    14341 gccgagcagg ctggcgccgc ggcgccggtt gcgggcggca cggtcgagaa tgccggcgcg
    14401 gcggctggcg agggcgtggt gtacaaccgt ctcggccagc tcgcccgcca ggtccatgac
    14461 gcgctgcgcg atctcggcct cgacaccacg ctgcaccggg cggccgaggc cgtgcccgac
    14521 gcgcgccagc gcctcaccta cgtcgcccgc accaccgagc aggcggccag ccgggtgctc
    14581 aacgccaccg acgtggcagg gccgatccag acggaactcg cgaacggggc cgatgcgctg
    14641 tgcgcgcgct gggacgaact ctacgccggc cgcctgagtc ccgaggcctt ccgcgaactc
    14701 tccgccgaca cgcgcgcctt cctcggcacg gtgggcgacg gcgcgcgcag caccggcgcg
    14761 cagctgcacg agatcatgat ggcgcaggac ttccaggacc tcaccggaca ggtgatcaag
    14821 cgcctggtcg agctcacccg cacgctcgag acccagctgc tgcaggtgct gatcgaggcc
    14881 gcgccggcgc aggtgctcga gcccgagcag gcggccgggc tgctcaacgg accggtgatc
    14941 gacccggccg ggcgcagcga cgtggtgcag gaccaggccc aggtcgacga gctgctcgag
    15001 cgtctcggat tctgaccaag ccgcggggcc ggaccccgcg cgaaccggtg atcgtcccgc
    15061 gtggggtcgc accgcaacca ggagcaagcc gtgagtgatt ttgccgggat ggaggatctg
    15121 ctgcaggact tcctcgtcga agccggcgac ctgctgtccg acgtcgacaa caagctcgtc
    15181 gaactcgagc acagcccgga tgaccccggc ctgctcaacg acatcttccg tggcttccac
    15241 accatcaagg gcggcgcggg cttcctcggc gcggccgagc tggtgaccct gtgccacctc
    15301 accgagagcc tattcgatcg cctgcgcaag cacgagctcg aggtcacccc cgagcgcatg
    15361 gacgtgatcc tgtcggcgac cggtgcggtg cgcgacatgt tcgtcgacct cggccgcggc
    15421 agccggcccg gtccggcggc gcccggcctg ctcgatgcct tg
//
LOCUS       DAOQNN010000030        15347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1534_length_15347_cov_9.665773, whole genome shotgun sequence.
ACCESSION   DAOQNN010000030 DAOQNN010000000
VERSION     DAOQNN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 15347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15347
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1534_length_15347_cov_9.665773"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKV97_03490"
     CDS             complement(<1..325)
                     /locus_tag="PKV97_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04968.1"
                     /translation="MVLTRSFVRAKRPCTDGFRWFVRQFGEGGDYQAMLDEMVAAGRV
                     GDACWLLEQFGPTDELRVVDAIEADAIVFAGTLEVRGGVDVDGVLRVGRSLLAGAGVR
                     AGGEVH"
     gene            complement(545..724)
                     /locus_tag="PKV97_03495"
     CDS             complement(545..724)
                     /locus_tag="PKV97_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04969.1"
                     /translation="MTLPARLRSAHRRAALRAGVRVRARGIVSPLRDLATQLAFKVTG
                     SPAVLSQAVRGHQRG"
     gene            1009..2307
                     /locus_tag="PKV97_03500"
     CDS             1009..2307
                     /locus_tag="PKV97_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB04970.1"
                     /translation="MSGQFRLLRERRFLPFFLTQFLGAFNDNVYKNALVVLITFQAAR
                     LGADSPGVLVNLAAGVFILPFFLFSATAGQLADKYEKSRLIRATKLLEIAVMALAVVG
                     FAQMSLPLLLVVLFLMGAQSALFGPVKYAIIPQQLADDELVGGNALVEAATFVAILAG
                     TIVGGLLVAGDAGPGRVAVAVLAIALLGWWSSRAIPPAAAADPGLRVNWNPVTQTREM
                     LRFMLEARAVFVAIVGISWFWFYGAVFLSQFPGFAADHLGGDERAVTLLLALFSVGIG
                     AGSLLCGRLSRGRVEPRMVLPGAIGLSLFALDLWWASPAAGAFPPGQGLDALFARAEV
                     WRVVFDLVMIGVCGGFFIVPLYALVQQRSAPAHRARVIAGNNILNALFMVAAAAMGVG
                     LLAAGFAVPQLFLATALLNALVAALLFAREPAFRQRGGPP"
     assembly_gap    2413..2512
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2611..4794
                     /gene="ovoA"
                     /locus_tag="PKV97_03505"
     CDS             2611..4794
                     /gene="ovoA"
                     /locus_tag="PKV97_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HNB04971.1"
                     /translation="MNPASQATPGDRPSPASSSAGSPAPLFPRTPLLSGTDVEAKRAE
                     LLHYFHATFDRYESLFEVLTCDEAYYRKPISLRHPLIFYFGHTATFFINKFMLAGLIE
                     QRIDPRLESMFAVGVDEMSWDDLDDARYDWPSVAEVAAYRGKVRAVVERVIRDTPFTL
                     PIGWKDPFWAVVMGIEHERIHLETSSVLMRQHALQYVKPHPAWQPCAEHGEPPANALV
                     DIPAGTVTLGRSFDEPIYGWDNEYGTHRAEVPAFQAARHLVTNREYLGFVEAGGYADD
                     SVWDEEGLGWRRFARAEHPTFWVPDAAGWKLRLMTEEVPMRWDWPVEVNCLEARAFCR
                     WKARETGQPVRLPTEDEWNRIHDHAGLADVPHDAPANANLHLDHWASSCPVTRFAHGE
                     LFDVVGNVWQWCETPTYPFDGFDVHPIYDDFTTPTFDDRHAIIKGGSWISAGNESRHA
                     SRYAFRRHFFQHAGLRYVVSEAPVINPASSYETDTLLSQYAEFHYGDEAFGVPNFPKA
                     LADIAIAAQRRFGKGRFGRALDLGCATGRASFELARAFERVVGIDFSARFIQAGVKLA
                     ETGVLRYTMPDEGELVTYHERRLDALELAGTAGRVEFWQGDACNLKDVFTGFDLILAA
                     NLIDRLYSPRRFLADVPRRLKPGGLLLLASPYTWLEEHTKREEWIGGFKKDGESYTTL
                     DGLKDLLAADFELVQGPQAVPFVIRETRRKHQHTLSELTVWRKRS"
     gene            4973..5284
                     /locus_tag="PKV97_03510"
     CDS             4973..5284
                     /locus_tag="PKV97_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04972.1"
                     /translation="MNPLVDSILRGGALAVLALAGGCAHVWVDAEGQRHLLGWMHLTL
                     PPAQGAVAGETLRVQTLGLGWTRAEAGSALVLGYADTTLGFLRDGACLAPAADRTEVV
                     R"
     gene            5281..>6185
                     /locus_tag="PKV97_03515"
     CDS             5281..>6185
                     /locus_tag="PKV97_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04973.1"
                     /translation="MSTRRVSILLSLALMVAGCASRPDTVVFVTKSSLGVDVEQTPPS
                     ASIAYDRVEGYFGPRYESGAVPPVLAVFMTNGQLLDRQVKQLYATGDAARALLAPAAP
                     LPSDPEQGGDFKPMFFGSSTTVGLKIGYEAGGSTSTFTLGYKRKELSVIPVNAGRFPS
                     VLATLQTDVDARSATDSRFGAGQLFATGAAATALAARPEVRDVFLTKADAALNAYRSE
                     ERQQSRHALVTLGCLAALPDAKLPAVWRNAEALGVLDQEAGAGAFASLGPDAARERYT
                     RYLALLDADSADATTASPEHPQWEK"
     assembly_gap    6186..6195
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6263..7405
                     /locus_tag="PKV97_03520"
     CDS             6263..7405
                     /locus_tag="PKV97_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNB04974.1"
                     /translation="MERIIPCTDCAEKTLDVEQLGFRVTSCDPHPERPGFCVLRFEDR
                     SATPAAGASLAAPAAAARAGRGAVAGGVTATQAAVAKAIVNLFETGEVLGQYGKVTLI
                     PGDTGHLTFGRSQTTLGSGNLAKLLQQYCANPGARFAGRLASYLPRFLAIDESLDDDP
                     RLHNVLRATADDPVMRDTQDAFFDRTYWEPALRAAASLGVHTPLGVAVVYDSAVHGSW
                     LAMRDRTTRAVGEPAAVGEQAWIDAYVRTRRAWLEGHARADLRQTVYRMEAFGRLIDQ
                     GFWGLEMPLVVRGREISSVTLAALPPGCYDGPQPGSRPLTLATPLARGLDVRLLQLGL
                     SDRGVDILADGIFGRTSFNLLKAWQAQHGLAATGIADPALIGELTA"
     gene            7418..8041
                     /locus_tag="PKV97_03525"
     CDS             7418..8041
                     /locus_tag="PKV97_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04975.1"
                     /translation="MKRAKTLWMVALAVLLLVAGLYAGERRGVPAPAPAPARTAVEEI
                     VLVANEVVEAFARRDGERLAGRAHPHKGVRFSPYAYVDVEEDRVFLPAQLRHFWDDAT
                     VHVWGHADGSGEPIALTAAQYVERFVLDRDYRGAGRIGVNVDQQRGNTADNAAQAYPQ
                     GTRVEYYVEPAPGGGAAAFDWSALRLVFEQVDGRWLLVGVIHDQWTT"
     gene            8153..8278
                     /locus_tag="PKV97_03530"
     CDS             8153..8278
                     /locus_tag="PKV97_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04976.1"
                     /translation="MNGTTEKREPDEQEIDVCDRPVQETEATPDEDLPAAEGGVA"
     gene            8376..9104
                     /locus_tag="PKV97_03535"
     CDS             8376..9104
                     /locus_tag="PKV97_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNB04977.1"
                     /translation="MGVVKGVICHHTAGPRSGNMPSLGVLKNGRADLAGPLAQLGLGR
                     DGTFYVIAAGRANHAGRGEWKGITDGNGSFIGIEAESTGIGDPTKPGFEPWPEVQLDA
                     YRRGVAAILAHLGQDAGMCCGHKEYALPKGRKSDPHTLDMERFRSEVAALMSGAAAPV
                     PSIPRKDAEDRPTIRRGATGNPIFLVKEVQRKLGFGAQQVDGAFGPLTEAAMRRFQRA
                     HGLVPDGIVGPRTWKALDTVAIGP"
     gene            9104..10216
                     /locus_tag="PKV97_03540"
     CDS             9104..10216
                     /locus_tag="PKV97_03540"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNB04978.1"
                     /translation="MSADPVSVAGIDGPAALRDRALGALLGAFIGDALALGPHWYYDL
                     DELRARYGPWITGYTAPMPDHYHAGMKAGESSQAGVLLRLTMESLAECGGYDEADFCR
                     RMDEDFFPRLDGTPMQGPGGYTSQSIREAWRKRIRQGLPWGRVAGNADTTEAAERTLA
                     IAVRYALDPAALADAVTRNTVLTQTDGTVVAMTVAFGAVLGMLVEGHKFDAAISAKLM
                     ARVKSGALPFHTVTAGNLQAPRPGAAEAPTAGRFPSPDALLTQSSIARAAHDPDVRIE
                     PAWKVALVYGMPCAVYHQFPAAYYLAARFADDFESAVLHALNGGGQNLARTMLTGALV
                     GAQVGLAGIPTRFVKGLVDHAALLALAGRIGPGRVL"
     gene            10433..10981
                     /locus_tag="PKV97_03545"
     CDS             10433..10981
                     /locus_tag="PKV97_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HNB04979.1"
                     /translation="MTPIRAAALALVLTCAVPAAHALDDLRPGLWEFRSTRLSLAGLP
                     DMAAQMGMLQQHIKGLPADTRRMLEQQMQQRGVSLGADGSVRSCITPAQARQDNVFAG
                     KVEGNCTLTRVERSGNTIRGSLRCTDPDAEGDFHARVEGPEHFTTRVKLASVRGDLDL
                     ETDARWLGAGCGAAAVAPKPAR"
     gene            11128..11403
                     /locus_tag="PKV97_03550"
     CDS             11128..11403
                     /locus_tag="PKV97_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04980.1"
                     /translation="MKSHLAEDIRIASPRFHLPTRDGLYAPIAFLFVTERMRDDILNE
                     RSLLVASLPPALRARQQKLFDRYDPVAGARSFTELLELYRYPFAGSH"
     gene            11568..11843
                     /locus_tag="PKV97_03555"
     CDS             11568..11843
                     /locus_tag="PKV97_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB04981.1"
                     /translation="MKSHLAENIQIAHPRYHLSSDDGLYRPIPFLFVSPRMRDDILDE
                     REMLLSAQPAALHERQQKLFASYDPAVSMEAFRQLLRLYGYPFNNRR"
     gene            complement(11874..12734)
                     /locus_tag="PKV97_03560"
     CDS             complement(11874..12734)
                     /locus_tag="PKV97_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNB04982.1"
                     /translation="MHSLLEFPSPFPAEPGVVRLLEPPGSDAAALAARLVAGDYGKPF
                     VLENGGLRALHFSLGYVQSVMDIANPSALVLAYARAMMAFLLFNPRPRRIGMIGLGGG
                     SLANFCHRHLAAAELTVVEIDADVIALRRHFGVPADDARFRVLHADGVRWLGETQTRS
                     DVLLIDAFGAEGVAHTLLDTDFHANAFRHLGGHGVLVMNLAGARESFAPHVARLLEIF
                     DERVIAIPVREDGNHILFAFRDPAFTPRWNWMRSQAHALQARTGLDFPNLARALEAGE
                     RQGHAWRLAR"
     gene            complement(12818..14263)
                     /locus_tag="PKV97_03565"
     CDS             complement(12818..14263)
                     /locus_tag="PKV97_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HNB04983.1"
                     /translation="MEAFANQVVDLLNGLLWGKILIWLLVGTGIYFTLRLGFIQLRHF
                     GHTFTVLRGSRQSDASGISSFQALCTSLAARVGTGNIAGVAVAITLGGPGAIFWMWVI
                     ALVGMATGFAEATLAQLFKQRDDKGQFRGGPAYYMEKGLGQRWAGTMFSIFLIIAFGF
                     AFNSVQSNTIAGAMVGAFELDMGSFTLGGNEVAVAALVVGVAVTVLTAVIIFGGLRSI
                     ARFSELAVPFMAVGYLVVAVGILLVNLGEVPGVLAMIVKSAFGFHEAAAGGIGAAILN
                     GFKRGLFSNEAGMGSAPNAAAAATPYPPHPASQGYVQMAGVFIDTILICTASAAIILL
                     AGPVEGTGVGLVQNALTSEVGGWGKYFLAVVVLFFAFTSIVANYFYAENCLVFIEHNH
                     PAGLLIFRLIVLAMVMFGAIGSLPFVWNFADVAMGLMAITNLVAILLLSGLVVKLAKD
                     YNAQRAAGKLPTFDARAYPEIRSKLTPGIWD"
     gene            14607..15347
                     /locus_tag="PKV97_03570"
     CDS             14607..15347
                     /locus_tag="PKV97_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB04984.1"
                     /translation="MAGGVALVELGSALVAPQASLDGVPVLVAGRPGGWTAVVGIALE
                     AEPGSVDLEVRAEGEPPRRVPVAVGPVRYAEQRLKVAPGKVDLSPADLARHERERLRS
                     AEIVATWSTLDPGSLRMLQPVPGRRSGSFGLRRVFNDQPRKPHGGMDIAAPTGTPVVA
                     AAAGRVIDTGDYFFNGNTVWLDHGRGLLTMYCHLSSIAVKAGDTVKAGARIGAVGATG
                     RVTGPHLHWSVMLNRSMVDPALFLPGGG"
BASE COUNT         2213 a         5357 c         5410 g         2257 t
ORIGIN      
        1 tgtggacttc gccacccgcg cgcaccccgg caccggccag cagcgagcgg ccgacacgca
       61 gcacaccgtc gacgtccacg ccgccgcgca cctcgagggt gccggcgaac acgatcgcgt
      121 cggcctcgat cgcgtcgacc acgcgcaact cgtcggtggg gccgaactgc tcgagcagcc
      181 agcaggcgtc gccgacgcgg ccggcggcga ccatctcgtc gagcatggcc tggtagtcgc
      241 cgccttcgcc gaactggcgg acgaaccagc ggaatccatc ggtacagggc cgcttcgccc
      301 tgacgaaact tcttgtgaga accatcgcat caccctcgtg catcgcatcg ccacgcggcg
      361 ggcgtggcgt tccgggtgca gccccgtggg gcagtgccga tggccgggag ggtccggatg
      421 gaccggggcc ggaccgtcag tctggtcgat gggcgggacg ctggagaaag acccgcggcg
      481 gctgcacgtt gaacgggagg cgcggcgtgc gcgtcccggg caaggtgccg tgcgggggtg
      541 gggctcagcc cctttggtgg ccccgcacgg cctgggaaag cacggcggga gaccctgtga
      601 ccttgaatgc gagctgggtc gcgagatcgc gcagcggcga cacgatgccg cgcgcgcgca
      661 cacgcacgcc cgcgcgcaac gcggcgcggc gatgggcgga acgaaggcgg gcgggcaggg
      721 tcatgcggcg ctcgatcggt ggaaagaggc gacattgtag gctaggaggt ggggcattgt
      781 cccccccgcc cggtccgcgc cgccccggct cggcggattc ggcgccgaag atcgcgaatg
      841 cgccgttcct tcgacaagga tgcatcccgc gtcgccgcgg ggcaggtgac gcggcggcgg
      901 tggcgaccgg gcccatttcc gattaccttg ccgggcgatg aagactcccg ccaatcccat
      961 ctccgcgagc ggccgcacgg cgcttgccca tttccgcgag gccgcacgat gagcggccag
     1021 ttccgcctgc tgcgggagcg ccgcttcctg cccttcttcc tgacccagtt cctcggtgcc
     1081 ttcaacgaca acgtctacaa gaacgcgctg gtggtgctga tcaccttcca ggccgcgcgt
     1141 ctgggggcgg actcgcccgg ggtgctggtg aacctcgccg cgggtgtctt catcctgccc
     1201 ttcttcctgt tctcggccac cgccggccag ctcgccgaca agtacgagaa gagccgcctg
     1261 atccgcgcca ccaagctgct cgagatcgcc gtaatggcgc tcgccgtcgt cgggttcgcg
     1321 cagatgtcgc tgccgctgct gctcgtcgtg ctgttcctga tgggggcgca gtcggcactg
     1381 ttcgggccgg tcaagtacgc gatcatcccg cagcagctcg ccgacgacga gctggtgggc
     1441 ggcaacgcgc tggtggaggc ggcgaccttc gtcgccatcc tcgccggcac catcgtcggc
     1501 ggcctgctgg tggcggggga tgccgggccc ggccgtgtgg cggtcgcggt gctcgcgatc
     1561 gcgctgctcg gctggtggtc cagccgcgcc atcccgcccg cggcggcggc cgaccccggg
     1621 ctgcgggtga actggaaccc ggtgacgcag acgcgcgaga tgctgcgctt catgctcgag
     1681 gcgcgcgcgg tcttcgtcgc catcgtcggc atctcgtggt tctggttcta cggtgccgtg
     1741 ttcctgtcgc agtttcccgg cttcgccgcg gatcatctcg gcggcgacga gcgcgcggtg
     1801 acgctgctgc tggcgctgtt ctcggtcggc atcggcgcgg ggtcgctgct gtgcgggcgc
     1861 ctgtcgcgcg ggcgggtgga gccgcgcatg gtgctgccgg gggcgatcgg gctgagcctg
     1921 ttcgcgctcg acctgtggtg ggcgagcccg gcggcgggcg ccttcccgcc cggccagggg
     1981 ctggatgcgc tgtttgcccg cgccgaggtg tggcgggtgg tgttcgacct cgtgatgatc
     2041 ggggtgtgcg gcggcttctt catcgtgccg ctgtatgcgc tcgtgcagca gcgctccgcg
     2101 ccggcgcacc gcgcgcgcgt gatcgccggc aacaacatcc tcaacgcgct cttcatggtc
     2161 gccgccgcgg cgatgggcgt cgggctgctg gccgcgggct tcgcggtgcc gcagctcttc
     2221 ctggccacgg ccttgctcaa cgcgctggtc gccgcgctgc tgttcgcccg cgagcccgcc
     2281 ttccgccagc gcggcggccc cccgtaggag cgacgcgagt cgcgatcgca gcgctcgatc
     2341 ggcgagggcg cgaaagttct gggcggcctg atcgcgactt gcgtcgctcc cacaaaacca
     2401 ccgcatccgg tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcgtgtcg
     2521 ctgctgcgca ggatgccggc ccacgctaca ttcccgcgcc gaaacgtgga aagatgcagc
     2581 ccatccgtcc caatgacgag gacccccgca atgaaccccg catcgcaagc gacccctggc
     2641 gaccgtcctt cccccgcttc ctcttccgcc ggcagccctg cgcccctctt cccgcgcacc
     2701 ccgctgctct ccggcaccga tgtcgaggcc aagcgcgccg agctgctgca ttacttccac
     2761 gccaccttcg accgctacga gtcgctgttc gaggtgctca cctgcgacga ggcctactac
     2821 cggaaaccga tcagcctgcg ccacccgctg atcttctact tcggccacac cgccaccttc
     2881 ttcatcaaca agttcatgct cgccggcctg atcgagcagc gcatcgaccc gcgcctggaa
     2941 tccatgttcg ccgtcggcgt cgacgagatg agctgggacg acctcgacga cgcgcgctac
     3001 gactggccga gcgtggccga agtggccgcc taccggggca aggtgcgcgc ggtggtcgag
     3061 cgcgtgatcc gcgacacgcc cttcaccttg ccgatcggct ggaaggaccc gttctgggcg
     3121 gtggtgatgg gcatcgagca cgagcgcatc cacctcgaga cctcctcggt gctgatgcgc
     3181 cagcacgcgc tgcagtacgt gaagccgcat cccgcctggc agccctgcgc cgagcatggc
     3241 gagccgccgg ccaacgcgct ggtcgacatc cccgccggca cggtcacgct cggccgcagc
     3301 ttcgacgagc cgatctacgg ctgggacaac gagtacggca cgcaccgcgc cgaggtgccg
     3361 gccttccagg ccgcccgcca tctggtgacc aatcgcgaat acctcggctt cgtcgaggcc
     3421 ggcggctacg ccgacgacag cgtctgggac gaggagggcc tgggctggcg caggttcgcc
     3481 cgcgccgagc acccgacctt ctgggtcccc gacgccgcgg gctggaagct gcgcctgatg
     3541 accgaggaag tgccgatgcg ctgggactgg ccggtggagg tcaactgtct ggaggcgcgc
     3601 gccttctgcc gctggaaagc gcgcgagacc ggccagccgg tgcgcctgcc caccgaggac
     3661 gagtggaacc gcatccacga ccacgcgggc ctcgccgatg tgccgcacga cgcccccgcc
     3721 aacgccaacc tgcatctgga ccactgggcc tcgagctgtc cggtgacgcg cttcgcccac
     3781 ggcgagctct tcgacgtggt cggcaacgtc tggcagtggt gcgagacccc gacctatccc
     3841 ttcgacggct tcgacgtgca cccgatctac gacgacttca ccacgccgac cttcgacgac
     3901 cgccatgcca tcatcaaggg cggcagctgg atttccgccg gcaacgagtc gcgccacgcc
     3961 tcgcgctacg ccttccgccg ccatttcttc cagcacgcgg gcttgcgcta cgtcgtcagc
     4021 gaggcgccgg tgatcaaccc ggcctcgagc tacgagaccg acaccctgct gtcgcagtac
     4081 gccgagttcc actacggcga cgaggccttc ggcgtgccca acttcccgaa ggcgctcgcc
     4141 gacatcgcga tcgcggccca gcgccgcttc ggcaagggcc gcttcggccg cgcgctcgac
     4201 ctcggctgcg ccaccggtcg cgccagcttc gagctggcgc gcgccttcga gcgcgtggtc
     4261 ggcatcgact tctcggcgcg cttcatccag gctggcgtca agctcgccga gaccggcgtg
     4321 ctgcgctaca cgatgcccga cgagggcgag ctggtcacct accacgagcg ccggctcgac
     4381 gccctcgagc tggccgggac cgccggccgc gtcgagttct ggcaaggcga cgcgtgcaac
     4441 ctcaaggacg tgttcaccgg cttcgacctc atcctcgccg ccaacctgat cgaccgcctg
     4501 tacagcccgc gccgcttcct cgccgacgtg ccgcggcggc tcaagcccgg cggcctgctg
     4561 ctgctcgcct cgccctacac ctggctggaa gagcacacca agcgcgagga gtggatcggc
     4621 ggcttcaaga aggatggcga gtcgtacacg accctcgacg ggctcaagga cctgctcgcc
     4681 gccgacttcg agctggtgca ggggccgcag gcggtgccct tcgtgatccg cgagacacgg
     4741 cgcaagcacc agcacacgct gtcggagctg accgtctgga ggaagcgctc ctgagcctcc
     4801 ccgccgcgct cgtccgtcgt gccatgcggc ggggcgcggc cggcttgcaa gcccacgacc
     4861 gcgggctatg ctggaagcgt gacccgcatg gcggtgctcc cggagggccg gggatcgtca
     4921 ccgggcgaag cgcccacggt ggcttccgcc ggcgtggttc atcgagcctg acatgaatcc
     4981 cctggtcgat tcgatcctgc gaggtggagc gctggccgtg ctggccttgg caggcgggtg
     5041 cgcgcacgtg tgggtcgatg ccgaggggca gcggcatttg ctcggctgga tgcatctgac
     5101 cctcccgccg gcgcaaggtg cggtcgccgg ggagaccttg cgcgtgcaga ccctgggcct
     5161 gggctggact cgtgccgagg cgggcagcgc gctcgtgctg ggctatgcgg acaccacgct
     5221 cggattcctg cgcgatggcg cctgcctcgc gcccgctgca gatcgaacgg aggtcgtcag
     5281 atgagcactc gccgcgtttc gatcctcctg tcgctcgcgc tgatggtcgc cggctgcgcg
     5341 agtcgaccgg acaccgtggt cttcgtgacc aagagctccc tcggcgtgga cgtcgagcag
     5401 acgccgccat cggccagcat cgcctacgat cgcgtcgagg gttatttcgg cccgcgctac
     5461 gagagcggcg cggtgccgcc cgtgttggcg gtgttcatga ccaacgggca gctgctcgat
     5521 cgccaggtga agcagctcta cgccaccggc gacgccgccc gcgcgttgct cgcgcccgcg
     5581 gcgccgctgc cgtccgatcc ggagcagggg ggcgacttca agccgatgtt cttcggcagc
     5641 tcgaccacgg tgggcctgaa gatcggctac gaggccggcg gcagcacctc caccttcacc
     5701 ctcggctaca agcgcaagga gctgtcggtg atcccggtga acgcaggccg cttcccctcg
     5761 gtgctggcga ccctgcagac cgatgtcgac gcccgcagcg cgaccgactc gcgcttcggc
     5821 gccgggcagc tgttcgcgac cggcgccgcc gcgaccgccc tcgccgcgcg gccggaggtc
     5881 cgggacgtct tcctgaccaa ggccgacgcc gcgctgaacg cctatcgcag cgaggaacgc
     5941 cagcagtccc ggcacgccct ggtcacgctg ggctgcctgg ccgcgctgcc cgatgcgaag
     6001 ctgcccgcgg tatggcggaa cgccgaggcg ctgggggtcc tggaccagga agccggtgcc
     6061 ggcgcgttcg ccagcctggg cccggatgcc gcgcgcgagc gctatacccg ctacctggcg
     6121 ctgctggacg ccgattccgc ggacgccacg acggcatcgc ccgaacaccc gcagtgggaa
     6181 aaaaannnnn nnnnngtccc tgcaccgcca gtacgtgtgc ggccttgccg ggcgttgatc
     6241 cccgaacccg aaggagcgag ccatggaacg catcattccc tgcacggatt gcgccgagaa
     6301 gacgctcgac gtcgagcagc tcggcttccg ggtcaccagc tgcgatcccc atcccgagcg
     6361 gcccgggttc tgcgtgctgc gcttcgagga tcgcagcgcc acgccggcgg ccggggcaag
     6421 cctcgcagcc cccgccgcag cggcgcgcgc ggggaggggg gcggtcgccg gtggcgtcac
     6481 cgccacccag gcggcggtcg ccaaggcgat cgtcaacctg ttcgagaccg gggaggtgct
     6541 gggccagtac gggaaggtga cgctgatccc gggcgatacc ggccacctga ccttcggccg
     6601 atcgcagacc acgctcggct ctggcaacct cgccaagctg ctgcagcaat actgcgccaa
     6661 ccccggggca cgcttcgccg gccggctggc gtcctacctg ccgcgcttcc tggccatcga
     6721 cgagagcctc gacgacgatc cccgcctgca caacgtgctg cgtgcgaccg ccgacgatcc
     6781 ggtgatgcgc gatacgcagg atgcgttttt cgatcggacc tactgggagc ctgcgctgcg
     6841 cgcggccgca agcttgggcg tgcacacccc gctcggcgtg gcggtggtgt acgacagcgc
     6901 cgtgcacggc tcctggctgg cgatgcgcga ccgcaccacg cgcgcggtcg gcgagcccgc
     6961 ggcggtgggc gagcaggcct ggatcgacgc ctatgtgcgc acgcgacgag cctggctgga
     7021 gggccacgcg cgcgccgacc tgcgccagac ggtgtatcgc atggaagcgt tcggacgcct
     7081 catcgaccag ggcttctggg ggctcgagat gccgctcgtg gtgcgcggca gggagatctc
     7141 gagcgtgacg ctcgccgcct tgccgcccgg ctgctacgac gggccgcagc cgggttcgcg
     7201 ccccttgacg ctggcgaccc cgctggcgcg cgggctggat gtccgcctgc tgcagctcgg
     7261 cctgtccgac cggggcgtgg acatcctcgc cgatggcatc ttcgggcgga ccagcttcaa
     7321 cctgctcaag gcctggcagg cgcagcacgg gctggcggcc accggcatcg cggaccccgc
     7381 cctgatcggc gagttgacgg cctgaggggg cgggtcgatg aagcgcgcga agacgctgtg
     7441 gatggtggcg ctggccgtgc tgctgctcgt ggccggcctg tacgcgggcg aaaggcgcgg
     7501 cgtgccggcg ccagcgccgg ctccggcccg cactgcggtc gaggagatcg tcctggtcgc
     7561 gaacgaggtc gtggaggcct ttgcgcgacg cgacggcgaa cgcctcgcgg gcagggcgca
     7621 tccgcacaag ggcgtgcgct tctcgcccta tgcctacgtc gatgtcgagg aggatcgcgt
     7681 cttcctgccc gcacagctgc gccacttctg ggacgacgcc acggtccacg tgtggggcca
     7741 tgccgacggc agcggcgagc cgatcgcctt gacggccgcg cagtatgtcg agcgcttcgt
     7801 gctcgaccgc gactatcgcg gggcgggcag gatcggtgtg aatgtcgacc agcagcgcgg
     7861 caacactgcc gacaacgctg cccaagcgta tccgcaggga acacgggtgg agtactacgt
     7921 cgagcccgcg ccgggtggcg gggccgcggc cttcgactgg agcgccttgc gcctggtctt
     7981 cgagcaggtg gacggacgct ggctgctggt gggggtcatc cacgaccaat ggacgacctg
     8041 atcgggcctg gaatccgctt gtcggggcgc atcgacggcc tatattgaag ggtatcccgc
     8101 atgggcgtca ccgtcctgcg ggcgcaccca ggacaggaag gagggctgca agatgaacgg
     8161 aacgaccgaa aaacgcgaac ccgacgagca ggagatcgac gtgtgcgacc gcccggtcca
     8221 ggagaccgag gcgacgcccg acgaagacct tcccgccgcc gaaggggggg tagcctgaca
     8281 tgccattcca gctgacatgg ttgccggtcg ttctgaggga cgccggcttg aaggtcgcgg
     8341 aggtcgatgg ctggccgaac cgcggacgga gcgaaatggg tgtcgtcaag ggcgtgatct
     8401 gccatcacac cgccggtccg cggagcggaa acatgcccag cctcggggta ctcaagaacg
     8461 ggcgtgccga tctggcgggg ccgctggcgc agctcggtct cgggcgggac ggcacgttct
     8521 acgtgatcgc cgcgggaagg gccaatcacg cggggcgggg cgagtggaag ggcatcaccg
     8581 acggcaacgg cagcttcatc ggcatcgagg ccgagagcac cggaatcggt gatccgacca
     8641 aacccgggtt cgaaccctgg cccgaggtgc agctcgacgc ctatcgtcgc ggcgtggccg
     8701 ccatcctggc gcatctcggc caggatgccg ggatgtgctg cggtcacaag gaatatgcgc
     8761 tgccgaaagg gcgcaagagc gacccgcaca cgctcgacat ggagcgtttc cgcagcgagg
     8821 tcgcggccct catgagcggc gcggctgcgc ccgtgccctc gatcccgcgc aaggatgcgg
     8881 aggatcgacc caccatccgg cgcggcgcga cgggcaatcc gatctttctg gtgaaggaag
     8941 tccagcgcaa gctcggcttc ggcgcacagc aggtggatgg cgccttcggc cccctgaccg
     9001 aggcggcgat gcggcgcttc cagcgcgcgc acggcctggt gccggacggc atcgtcggtc
     9061 cgcggacctg gaaggcgctc gacaccgtgg cgatcggccc ctgatgtctg ccgacccggt
     9121 ttccgtcgcg ggcatcgacg ggcccgccgc cctgcgggac cgtgccctcg gtgccctgct
     9181 cggagccttc atcggcgacg cgctggcgct cggcccgcac tggtactacg acctcgacga
     9241 gctgcgcgcg cgctacgggc cgtggatcac cggctatacc gcgcccatgc ccgaccacta
     9301 ccatgcgggc atgaaggcgg gcgaatcctc gcaggccggc gtgctgctga ggctcacgat
     9361 ggagtcgctg gccgaatgcg gcggctacga cgaggccgac ttctgccgcc gcatggacga
     9421 ggatttcttt ccacgactcg acggcacgcc gatgcagggg cccggcggct acaccagcca
     9481 gtcgatccgc gaggcctggc gcaagcgcat ccggcagggc ctgccctggg gccgggtggc
     9541 cggcaacgcc gacaccaccg aggccgccga gcgcacgctg gcgatcgcgg tgcgctacgc
     9601 cctcgacccc gccgcgctcg ccgacgccgt cacccgcaac acggtgctga cccagaccga
     9661 cggcaccgtg gtggcgatga ccgtcgcctt cggcgcggtg ctcggcatgc tggtcgaggg
     9721 ccacaagttc gacgcggcga tctcggccaa gctgatggcg cgggtgaagt ccggcgcgct
     9781 gccctttcac accgtgaccg cgggaaacct ccaggcgccg cgccccggtg ccgccgaggc
     9841 cccgaccgcc ggccgcttcc cgtcgccgga tgcgctgctc acgcagtcgt cgatcgcgcg
     9901 cgcggcgcac gacccggacg tgcgcatcga gccggcgtgg aaggtcgcgc tggtgtatgg
     9961 catgccctgc gcggtgtatc accagtttcc ggcggcgtat tacctcgcgg cgcgcttcgc
    10021 cgacgacttc gagtcggcgg tgctgcatgc gctcaatggc ggcgggcaga acctcgcgcg
    10081 caccatgctg accggtgcgc tggtgggggc gcaggtcggt ctggcgggga tccccacgcg
    10141 cttcgtgaag gggctcgtgg atcacgcggc gctgcttgcg ctggcgggac ggatcgggcc
    10201 gggccgggtc ctgtaggcgc gacgcaagtc gcgatgcatg gcgtgccgat acccgccacg
    10261 ccgggctcct ccgccgctgt ccgccgcacc gcgctccttc gtgccgccgc aaaatccgct
    10321 cacgatccgg ggggctgcga ggcttcgtcg cccgcccccg gcctgcgata atgcaggcgt
    10381 cgatgtcggg cgcgcttccg cgcccgcacg ccgaaccacg aggaccgccc cgatgacccc
    10441 gatccgcgct gctgcgctcg ctcttgtcct gacctgcgcc gtgccggccg cgcacgcgct
    10501 ggacgacctc cgccccggcc tgtgggagtt ccgctccacc cgcctgagct tggccggcct
    10561 gcccgacatg gcggcgcaga tgggcatgct gcagcaacac atcaagggcc tgccggccga
    10621 cacccggcgc atgctggagc agcagatgca gcagcgcggg gtgagcctgg gcgcggacgg
    10681 cagcgtgcgc agctgcatca cacccgcgca ggcgcgccag gacaacgtct tcgccggcaa
    10741 ggtcgagggc aactgcacgc tgacgcgggt cgagcgtagc ggcaacacca tccgcggcag
    10801 cctgcgctgc accgatccgg acgccgaggg cgatttccac gcgcgcgtcg aaggcccgga
    10861 gcatttcacg acccgcgtga agctcgcctc ggtgcgcggc gacctcgatc tcgagacgga
    10921 tgcgcgctgg ctcggtgcgg gctgcggggc ggcggcggtc gcgccgaagc cggcgcgtta
    10981 gcgcgctgct gacggaaatc cgcgctcgcc ttttgcgtgc gagcgtgatg gattgcggcc
    11041 acaccgcgct ttcatgctac gcgttgcggg gcgtatgagg gatgattcca ggaaggacgg
    11101 agacccactg cctgcgagcc tggaaccatg aaaagtcatc tcgccgaaga catccgcatc
    11161 gccagcccgc gttttcacct gcccacccgc gacggactct atgcaccgat cgccttcctg
    11221 ttcgtgaccg agcggatgcg cgacgacatc ctgaacgagc gctcgctgct cgtcgcttcc
    11281 ctgcccccgg ccctgcgcgc acgccagcag aagctcttcg atcgctacga cccggtggcg
    11341 ggggcgcgca gcttcaccga gctgctcgag ctctatcgct accccttcgc cggctcgcat
    11401 tgagcggatc cacgccgctg ccgccgcgca cgcagcgtgt gcggcgggat cttttttccg
    11461 cttccatggt cgttcagacg gacgcgccgg ccctgccgcc cgcgtcgaca gctttccgag
    11521 cccgcgtggt gcgggacgga catcgatttc gaccgtacga cggagccatg aagagtcatc
    11581 tcgccgagaa catccagatc gcccaccccc gttatcacct ttcgtccgac gacggcctgt
    11641 accggccgat ccccttcctg ttcgtgagtc cgcggatgcg cgacgacatc ctcgacgagc
    11701 gcgagatgct gctgtcggca cagcccgcgg cgctgcacga gcgccagcaa aagctgttcg
    11761 ccagctacga ccccgcggtc agcatggagg ccttccgcca gctgctgcgc ctgtacggct
    11821 acccgttcaa caaccggcgc tgagtcggcg tcgagccgac ggcagccgcc cgctcagcgt
    11881 gcgagccgcc aggcatggcc ctgacgttcg ccggcttcga gcgcgcgggc gagattggga
    11941 aagtccagtc cggtgcgcgc ctgcagggcg tgggcttggc tgcgcatcca gttccagcgc
    12001 ggcgtgaagg cgggatcgcg gaaggcgaac aggatgtggt tgccgtcctc gcgcaccggt
    12061 atcgcgatca cgcgttcgtc gaagatctcg agcaggcggg cgacgtgggg ggcgaaggat
    12121 tcgcgtgcgc cggcgaggtt catgaccagc acaccgtgcc cgcccaggtg acggaaggcg
    12181 ttggcgtgga aatcggtgtc gagcagggtg tgggcgacgc cctcggcgcc gaaggcgtcg
    12241 atcagcagca cgtcgctgcg cgtctgcgtc tcgcccagcc agcgcacgcc gtcggcgtgg
    12301 aggacgcgaa agcgcgcgtc gtcggcgggc acgccgaagt gtcggcgcag ggcgatcacg
    12361 tcggcgtcga tctccaccac ggtcagctcg gccgccgcca gatggcggtg gcagaagttg
    12421 gccagcgagc ccccgccgag gccgatcatg ccgatccggc gtgggcgcgg attgaacagc
    12481 aggaaggcca tcatcgcgcg tgcataggcg agcacgagcg cgctgggatt ggcgatgtcc
    12541 atcacgctct gcacgtagcc gaggctgaag tgcagcgcgc gcaggccgcc attctcgagc
    12601 acgaagggct tgccgtagtc gcctgcgacg aggcgggcgg cgagcgcggc ggcgtcgctc
    12661 ccgggcggct cgagcaggcg caccacgccg ggctcggcgg ggaaggggct cgggaactcg
    12721 aggaggctgt gcatcggatc ggcggctgcg cggcgcgccc gctccgggac gcgcctggca
    12781 tccggccggg gtggcgccgc tcggggcgtg gccgggctca gtcccagatg cccggcgtga
    12841 gcttgctgcg gatctcgggg tacgcgcggg cgtcgaaggt gggcagcttg ccggcggcgc
    12901 gctgggcgtt gtagtccttc gccagcttca ccaccaggcc cgagagcaac aggatggcga
    12961 cgaggttggt gatcgccatc aggcccatcg ccacgtcggc gaagttccac acgaagggca
    13021 gcgagccgat cgcgccgaac atcaccatgg ccagcacgat caggcggaag atcagcaggc
    13081 ccgcggggtg gttgtgctcg atgaacacca ggcagttctc ggcgtagaag tagttggcga
    13141 cgatcgaggt gaaggcgaag aacagcacca cgacggcgag gaagtacttg ccccagccgc
    13201 ccacttcgct ggtcagggcg ttctgcacca ggccgacgcc ggtgccttcg accggaccgg
    13261 cgagcaggat gatggcggcg gacgcggtgc agatcaggat cgtgtcgatg aacacgccgg
    13321 ccatctgcac gtagccctgc gaggccgggt gcggcgggta gggcgtggcc gcggcggcgg
    13381 cgttgggcgc cgagcccatg ccggcctcgt tggagaacag gccgcgcttg aagccgttga
    13441 ggatggccgc gccgatgccg ccggcggcgg cttcgtggaa gccgaaggcg ctcttgacga
    13501 tcatggccag cacgcccggc acctcgccga ggttgacgag gagaatgccg accgccacca
    13561 ccaggtaacc gaccgccatg aagggcacgg cgagctcgga gaagcgcgcg atcgagcgca
    13621 ggccgccgaa gatgatcacc gcggtgagca cggtgaccgc cacgccgacc accagcgccg
    13681 cgacggcgac ctcgttgccg cccagcgtga agctgcccat gtcgagctcg aaggcgccga
    13741 ccatcgcgcc ggcgatcgtg ttcgactgca ccgagttgaa ggcgaagccg aaggcgatga
    13801 tcaggaagat cgagaacatc gtgcccgccc agcgctggcc gagacctttc tccatgtagt
    13861 aggcggggcc gccgcggaac tgccccttgt cgtcgcgctg cttgaagagc tgcgcgagcg
    13921 tggcctcggc gaagccggtg gccatgccca cgagcgcgat cacccacatc cagaagatcg
    13981 cgcccgggcc gccgagcgtg atcgccaccg ccacgccggc gatgttgccg gtgcccacgc
    14041 gcgcagcgag cgaggtgcac agggcctgga aggacgagat gccggaggcg tcggactggc
    14101 ggctgccgcg cagcaccgtg aaggtgtggc cgaagtggcg caactggatg aagcccaggc
    14161 gcagggtgaa atagatgccg gtgccgacca gcagccagat caggatcttg ccccagagca
    14221 ggccgttgag caggtcgacg acctggttgg cgaatgcttc catgtttggt ctccagccgc
    14281 gggtggcggc gtttcgtcat gaagcccgga tcgcgggcgg aaaaagcgag ggcgcgattc
    14341 tagcgtgcgc atcggcgatc tccgcgcttt tcgccgctat catcgcggca tcggtcgtcg
    14401 tcgcgatacc gtccgcagct tcatgctgca ggcgtggcac cgcgccggca cggccccccg
    14461 aatccaggaa tccgatgatg cgctcgttcc cttcgtgcgg gtggcgtata cgccttgccc
    14521 gcgttcgcct tgcccgcgtt ctggccgctg cggcggcctg cgttgccctg ccggtcgcgg
    14581 cgctggaact gccgcgcgag cgcgcggtgg cgggcggggt cgccctggtc gaactcggat
    14641 ccgcactggt cgcgccccag gccagcctgg acggcgtgcc ggtgctggtc gccgggcgcc
    14701 cgggcgggtg gaccgcggtg gtgggcattg cgctcgaagc cgagccgggc agcgtggacc
    14761 tggaggtgcg cgcggaaggc gagccgccgc gccgggtgcc ggtcgcggtg gggccggtgc
    14821 gctacgccga gcagcgcctg aaggtggcgc cgggcaaggt cgacctgtcg cccgccgacc
    14881 tcgcccgcca cgagcgcgag cgcctgcgct cggccgagat cgtcgccacc tggagcacgc
    14941 tcgatcccgg cagcctgcgc atgctgcagc cggtaccggg gcggcgctcg ggctccttcg
    15001 ggctgcgccg ggtgttcaac gaccagccgc gcaagccgca tggcggcatg gacatcgccg
    15061 cgcccacggg caccccggtg gtggccgccg cggcgggccg ggtgatcgac accggcgact
    15121 acttcttcaa cggcaacacg gtatggctcg accatggccg cggcctgctc accatgtatt
    15181 gccacctgtc ctcgatcgcg gtgaaggcgg gcgacacggt gaaggccggc gcacgcatcg
    15241 gcgcggtggg cgccaccggg cgcgtcaccg gcccgcacct gcactggtcg gtgatgctca
    15301 accgcagcat ggtcgacccg gcgctctttc tgcccggcgg cggctag
//
LOCUS       DAOQNN010000031        15071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1584_length_15071_cov_12.252064, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000031 DAOQNN010000000
VERSION     DAOQNN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 15071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15071
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1584_length_15071_cov_12.252064"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKV97_03575"
     CDS             complement(<1..301)
                     /locus_tag="PKV97_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNB04985.1"
                     /translation="MSRLKNDTFLRALLRQPTEYTPVWLMRQAGRYLPEYCETRKRAG
                     SFLQLCKSPAMACEVTLQPLARYDLDAAILFSDILTVPDAMGLGLYFAEGEGPRFE"
     gene            complement(402..1877)
                     /locus_tag="PKV97_03580"
     CDS             complement(402..1877)
                     /locus_tag="PKV97_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNB04986.1"
                     /translation="MSSDARARAYYPALNALGLIIMIFGLLMSFPLVVSAALGDGATL
                     AYDQGLFVTFVAGLLLWAATRSKRRDLRVHDGFLLVAATWVVLPLFGALPLLAYIPDL
                     GFTDAYFEAVSGLTATGATVLTGLEHLPISINLWRTFMHWVGGMGVIVLVVAILPLLG
                     IGGRQLYKAEVPTPMKDSSLTPRIAETAKGLWLTYVLLTIACGFSLWWAGLAPWDAVI
                     HAFSVAGLGGFSNRDASLGHFDSVGVELVTAGFALLAGLNYATHYLALVRRSSAPYRR
                     DPEIPFYFGVLALSTLMLTAYLLAQGIFTDFGEALRYVAFHVVSISTSLGLATHDYTL
                     WPMFAQIWILFLGSFVACSGSTGGGIKMMRAMILYKQVIREIVRSLHPNAVRPVRMGR
                     NPVPEHILHAVLGFSFMYMVSIVSLTLVLSAAGLELVTAFSAVVACLNNTGPGLGLVG
                     PASNYQGLDDLHKWVLCFAMILGRLEIFTFLVVLTPTFWRR"
     gene            complement(1879..3282)
                     /gene="trkA"
                     /locus_tag="PKV97_03585"
     CDS             complement(1879..3282)
                     /gene="trkA"
                     /locus_tag="PKV97_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNB04987.1"
                     /translation="MKIIILGAGQVGASVAENLVSEANDITLVDTSEEYLGALRERLE
                     IRTVCGNGASPRVLREAGADDADLLIAVTQSDQTNLCACRIAKALFNLPTRIARLRST
                     DYVDHPELLDDDNFAVDFSICPEQVVTDYIKRLIAFPEALQVLEFADGVVSLIGVRAF
                     EGGPLVGHPLKALRFHLPNVEVRIAAIYRNGEPIMPEGDTIILPGDEVFCLAATVHIR
                     QVMRELRRSDRPMRRIMIAGGGNIGFRLAQSIEENHHVKILELNRTRAEMLASHLSNA
                     LVLRGDSTDEKLLETEGIDETDLFVALTNDEEDNIMSASLAKRMGARRTLALINRKSY
                     VDLVQGGPIDIAISPAHTSIGTLLAHVRRGDVVAVHSLRRGAAEALEIIAHGSSKDSK
                     VVGRPIEQIALPRGASIGAVVRDEKNEEGRVVRREVIIPHHDTVIREDDHVIVFCTNK
                     KLVQKVEKLFQVGFHFL"
     gene            complement(3413..4660)
                     /locus_tag="PKV97_03590"
     CDS             complement(3413..4660)
                     /locus_tag="PKV97_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB04988.1"
                     /translation="MAKILIVDDEIGIRELLSEILRDEGHDILLAENATAARAARNAV
                     RPDMVLLDIWMPDTDGITLLKEWAANGQLNMPVVMMSGHGTIDTAVEATRIGAIDYLE
                     KPIALQKLLAAVKRGLQRPQAPGVQSAPTLAAFHRSVPLRELRRRLEQIVGSSRVLLL
                     RVGAGSLAELVARSVQPANAPWLDLAAVTAPLDITQLQSTQGGVVFVGELARLSRAQQ
                     KNLAFALDRLERYDLRLVVATDRSLEALVGEGWEEGLLARLFEVSLAPPSIADVREDL
                     PELASQMLLHLVEAGEVPLRRFSTAALNQLRNLPWPGGYPELRAAVKSLALGTLEEEI
                     GAGEVRRLLPPDLGAGACGVSLDQPLREAREAFERMYFEHHLRLEGGNMTRLAEKTGL
                     ERTHLYRKLKQLGLQAGRRHEES"
     gene            complement(4670..6787)
                     /locus_tag="PKV97_03595"
     CDS             complement(4670..6787)
                     /locus_tag="PKV97_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB04989.1"
                     /translation="MKRVLLAVAAALSGISLFLLTSASSNTTLFATSYPWLLALNGGM
                     AVVLAALVGMQLHRLLREHREGQFGSRLKMRLVLMFALMGVVPGLVIYGVSLQFVVRS
                     IESWFDVRVDSALEGGIALGQNALDYLVSQVRDKADDMVLELEGAGQGAGTRLNRLRE
                     QAGIASATILAPAGQVLATAVESGRTLLPELPGTAELRQARQSRHFHSVIAEPDGRLV
                     IRVLTPIPVRTLAGESNLLMLQQPVPESFGRNAEAVQEAYREYQQLTLGRSGLKRIYT
                     VTLSLTLLLALLGALAVAVLIARRLAQPLLILAEGTQAVAQGDFSPRQALPANDELGV
                     LTQSFNRMTRQLQEARAAADRNRAEVEAARAYLESVLANLSTGVLAFSADGHLRAANH
                     GALQILEDDLGGFEDIPLEAWPRQAELGHMLREGFTANTGDWQKQMELPVRDRPPRTL
                     LIRGSRLPESTGGGLVVVFDDISSLIAAQRTAAWGEVARRLAHEIKNPLTPIQLSAER
                     LSFKLADRLDDNGREMLERATTTIVNQVEAMKNLVNAFRDYARLPAPQLAPLDLNALI
                     REVLHLYESAAVQIRTELGGALPPVQGDPTQIRQVIHNMLQNAQDALGAQEDAEISVI
                     TRRDGESALLLCRDNGPGFPPEVLARAFEPYFTTKSRGTGLGLAMVKKIVDEHGGEVR
                     LSNREGGGAEVRIRLRLAENKEA"
     gene            complement(6784..7323)
                     /locus_tag="PKV97_03600"
     CDS             complement(6784..7323)
                     /locus_tag="PKV97_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HNB04990.1"
                     /translation="MAALLLLWALSVRAESVIRSAEIVPGDGGYVLNADIAIDLNPRL
                     IDVVTRGVSLYFTTEVLIERPRWYWLNETVARRSLDYRLYYHAITRSFRLSVGNLHQN
                     FDELDEALRTMQRVRNWLVAETGVLQSGVSHEVSLRFRLDTSQLPKPFQVTAIGSNDW
                     NVETEWVRWTFLPGAPGFR"
     gene            complement(7343..8650)
                     /gene="rsmB"
                     /locus_tag="PKV97_03605"
     CDS             complement(7343..8650)
                     /gene="rsmB"
                     /locus_tag="PKV97_03605"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNB04991.1"
                     /translation="MRGAPRRAPEQPIDSLGYALARATELVAGVIDGANLTDAFERMQ
                     AGHPEWPEGTRGAVRDLAWSTLREFGRGDAILSRLLHSPPPVEIRALLLVALQRLTQR
                     PEQAHTVVDQAVGATAVAMPGLRNLVNGVLRNALRRQPEWQGWIEAEPEARHAFPAWW
                     VERVRSAHPQAWQDLLAAGNTRPPMALRVNPRRATLAEVEAELAAAGLEFRRLDNDAL
                     VLARPLAVARLRGYAEGRLSVQDAGAQWAAQLLDVRAGERVLDACAAPGGKTAHILER
                     ADADLLALELDPLRAGRVARNLDRLGLRAELKVADCRRLAAWWDGRPFDRILADVPCS
                     ASGVVRRHPDIKWLRRDSDIANFAAQQAEILEALWRTLAPGGTMLYVTCSVFDEENAG
                     QVARFCVRHADAERLPIRGSSDLQLLPCADHDGFYYALLRKRP"
     gene            complement(8759..9649)
                     /gene="speE"
                     /locus_tag="PKV97_03610"
     CDS             complement(8759..9649)
                     /gene="speE"
                     /locus_tag="PKV97_03610"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNB04992.1"
                     /translation="MSGASWFADLPKDVLAELLGEDAGYFLRGARLLESGRSEWQDYE
                     VWETPRFGKLFRLDGCFMTSERDEFHYHENLIHVPGVAHPGLRRALLIGGGDGGSAEE
                     LLKYPGIERIVIAELDAKVVDIARRYLDAVHHGALDDPRVEIRIGDGMAHVCEHAPAA
                     GERYDLIVLDLTDPIGPAEALYTADFFAACKALLAEGGALTLHIGTPVFQPERVRELL
                     ARLRRVFARVRPYFLYIPLYGSLWGMACASDTLDPAALDANEVDRRLAERAIRELQHF
                     NGAVHCAQFALPNHLRALLA"
     gene            complement(9787..10557)
                     /locus_tag="PKV97_03615"
     CDS             complement(9787..10557)
                     /locus_tag="PKV97_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNB04993.1"
                     /translation="METEFLFLALGVAALFAGFVDSIVGGGGLIQLPALFAAFPTTAP
                     ATLFGTNKLASIVGTTSAAVQYSRRVEIPWRVAAPGALAALVGSWYGAKAVAYLDPAI
                     LRPLILALLVLVAVYTFLRKDLGAVSREPEHGARSVAIGLGVGGVIGFYDGFFGPGTG
                     SFLIFLFIRLLGMDFLRASVSAKIVNVATNLAAISFFVGNVELMWKLAALMAVCNLSG
                     SILGSRMALRHGTGFVRKMFLAVVAVLILRLAYDTFLR"
     gene            complement(10731..12077)
                     /gene="mnmE"
                     /locus_tag="PKV97_03620"
     CDS             complement(10731..12077)
                     /gene="mnmE"
                     /locus_tag="PKV97_03620"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNB04994.1"
                     /translation="MESRPASPPDTIAAIATAPGRGGIGVVRVSGAGLGAFALALCGR
                     TPQPRIAHFTRFLDEHERPIDEGILLYFAAPASFTGEDVLELQGHGGPVVMQMLLERC
                     LQLGARLAEPGEFTRRAFLNGKLDLAQAEGVADLIEASTAAAARSAVRSLSGQFSEEV
                     HRIVDALIDLRMLVEATLDFPEEEIDFLERARAMPRLESIREALEGVLERARQGALLR
                     SGLDVVLVGAPNVGKSSLLNQLAGEERAIVTDIAGTTRDALRETIQVEGIPLHIIDTA
                     GLRETEDRVERIGIERTWREIARADVILHLVEAGVASEGDLDERLPQGVERIVVANKI
                     DLAGLPAGRVEAGGRVRLQVSARSGEGVELVRQELLRIAGWHAHGEDVILARERHLLA
                     LREALRHVEAAGTQLGALELFAEELRLAQEELGTITGEFSADDLLGVIFARFCIGK"
     gene            complement(12049..13707)
                     /gene="yidC"
                     /locus_tag="PKV97_03625"
     CDS             complement(12049..13707)
                     /gene="yidC"
                     /locus_tag="PKV97_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNB04995.1"
                     /translation="MDQRRLILFLIFSFSLVMLWDGWIKQNQPPVAQQQAAAGSAAPA
                     DGAVPTPTLSATPGQPVVPGEQAAQAAAAPRAVVETDLLRAEVSAQGGDIVRLELKQH
                     KASGEAGGNFVLLDDGTTRHQYAAQSGLIGEGLPTHKTLFQLPAGELRLKDGENGVEL
                     RLQAPEQNGVQVTKVMRFARGSYLIDVSYEIRNGSGQALAPHAYFQLTRDGNPAEQVE
                     AFGVRTFTGPAFYTDAAKFQKVTFEHITDGSAKFAKTASDGWVAMVQHYFVGAWLPEQ
                     GLQREYFARALGNNLFSAGVILPVTAIAPGAEGTVSNRLYAGPQEQDKLEGIAPGLDL
                     VVDYGWLTVIAAPLFWVLSWFHNLTGNWGWAIILVTVAIKAIFFPLSAASYKSMAKMR
                     VLGPRLQRMKEMYGNDKAKMQQEMMNLYRTEKINPLGGCLPILVQIPVFIALYWVLLG
                     SVEMRHAPWLGWIQDLSAKDPYFILPIIMGVSMLVQMKLNPTPPDPIQAKVMMAMPII
                     FTFMFLWFPSGLVLYWVVNNCLSIAQQWQITRMIESGKSAGKPA"
     gene            complement(13728..13937)
                     /gene="yidD"
                     /locus_tag="PKV97_03630"
     CDS             complement(13728..13937)
                     /gene="yidD"
                     /locus_tag="PKV97_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNB04996.1"
                     /translation="MKTVLIALLRFYRYAISPMLGRNCRFHPTCSEYAIEAVQRHGAL
                     RGGWMAAKRVGRCHPFNPGGYDPVP"
     gene            complement(13934..14296)
                     /gene="rnpA"
                     /locus_tag="PKV97_03635"
     CDS             complement(13934..14296)
                     /gene="rnpA"
                     /locus_tag="PKV97_03635"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNB04997.1"
                     /translation="MVSPTGADQGFRSAHRLHKTDEYSSVFAFRRALRGRFYMLHYRP
                     NGLDTARLGVVVAKKLAKRANVRNLVKRIAREIFRRQRIELPAMDLVVRLNAPVAKAT
                     RAELNLDLLSLLQRLPRQ"
     gene            complement(14298..14432)
                     /gene="rpmH"
                     /locus_tag="PKV97_03640"
     CDS             complement(14298..14432)
                     /gene="rpmH"
                     /locus_tag="PKV97_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNB04998.1"
                     /translation="MKRTYQPSVVRRKRTHGFLVRMKTRGGRAVIRARRAKGRHRLAV
                     "
     gene            14751..>15071
                     /locus_tag="PKV97_03645"
     CDS             14751..>15071
                     /locus_tag="PKV97_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HNB04999.1"
                     /translation="MTQDFWTFCLSRLEQELPQQQFNTWIKTLRAEPGEGGWALIAPN
                     RFVLQWVRERYLRRVEELGEEFAGAPLAIELQLPPAGAARPARAAATADVAPARASAP
                     RSTIE"
BASE COUNT         2601 a         5088 c         5147 g         2235 t
ORIGIN      
        1 gctcgaagcg cgggccctcg ccctcggcga agtacaggcc gaggcccatc gcgtcgggca
       61 cggtgaggat gtccgagaac aggatcgcgg cgtcgaggtc gtagcgcgcg agcggctgca
      121 gggtcacctc gcaggccatc gccgggctct tgcacagctg caggaagctg cccgcgcgct
      181 tgcgcgtctc gcaatattcg gggaggtagc gcccggcctg gcgcatcagc cagacggggg
      241 tgtattcggt gggctggcgc agcagggcgc gcaggaaggt gtcgttcttc aggcggctca
      301 cgtcgagtcc tctatcgggg cgttcatgca caagccggga ttatcgcctg tccggccccc
      361 gaacgggctt gtcgtcggtc aagcagtcgc ggcagggccg ctcagcgccg ccagaacgtg
      421 ggggtgagca ccaccaggaa ggtgaagatc tccaggcggc cgaggatcat cgcgaaacac
      481 agcacccact tgtggaggtc gtcgagcccc tggtagttgg acgccggccc caccaggccg
      541 agccccgggc cggtgttgtt gaggcaggcc acgaccgccg agaacgccgt caccagctcc
      601 agcccggcgg cggagagcac cagggtcagg ctgacgatgc tgaccatgta catgaagctg
      661 aagccgagca cggcgtgcag gatgtgctcg ggcaccgggt tcctgcccat gcgcaccggg
      721 cgcaccgcgt tcggatgcag cgagcgcacg atctcgcgga tgacctgctt gtacaggatc
      781 atcgcccgca tcatcttgat gccgccgccg gtcgagcccg aacaggcgac gaagctgccc
      841 aggaagagga tccagatctg cgcgaacatc ggccacaagg tgtagtcgtg cgtggcgagc
      901 ccgagcgagg tcgagatcga gacgacgtgg aaggcgacgt agcgcagcgc ctcgccgaag
      961 tcggtgaaga tgccctgggc gaggaggtac gcggtgagca tcagcgtgga gagcgcgagg
     1021 acgccgaaat agaaggggat ctcgggatcg cgccgataag gcgccgacga gcgccggacc
     1081 agcgcgagat agtgggtcgc gtagttgagt ccggcgagca acgcgaagcc ggcggtgacc
     1141 agctccaccc cgacgctgtc gaaatgcccg agcgaggcgt cgcggttgga gaagccgccc
     1201 aggccggcga cggagaacgc gtggatcacc gcatcccagg gcgccaaccc tgcccaccac
     1261 aggctgaatc cgcaggcgat ggtcagcaac acgtaggtca gccacagccc cttcgccgtc
     1321 tcggcgatgc gtggggtcag gctggagtcc ttcatcggcg tcggcacctc ggccttgtag
     1381 agctgacgac caccgatgcc gagcagcggc aatatcgcca ccacgagcac gatcaccccc
     1441 atgccgccga cccagtgcat gaaggtgcgc cacaggttga tcgagatcgg caggtgctcg
     1501 agcccggtga gcaccgtggc gccggtcgcg gtgagtccgg agaccgcctc gaagtaggcg
     1561 tcggtgaagc cgagatcggg gatgtaggcc aacagcggca gcgcgccgaa gagcggcagc
     1621 accacccagg tcgccgccac cagcaggaaa ccgtcatgca cccgcagatc acgccgcttg
     1681 ctgcgcgtcg ccgcccacag cagcaggccg gcgacgaagg tgacgaacag gccctggtcg
     1741 taggccaggg tcgcgccgtc gcccagcgcg gccgacacga cgagcgggaa gctcatcagc
     1801 agcccgaaga tcatgatgat caggccgagg gcgttgagcg cggggtagta cgcgcgggcg
     1861 cgggcgtcgg acgacatgtc agaggaagtg gaagccgacc tggaacagct tctcgacctt
     1921 ctgcaccagc ttcttgttgg tgcagaacac gatcacgtgg tcatcctcgc ggatcaccgt
     1981 gtcgtggtgc gggatgatca cctcgcggcg gaccaccctg ccctcctcgt tcttctcgtc
     2041 gcgcaccacc gcgccgatgc tcgcgccacg cggcagggcg atctgctcga tcgggcgccc
     2101 gaccaccttg gagtccttgc tgctgccgtg ggcgatgatc tccagggcct cggcggcgcc
     2161 gcggcgcagg ctgtgcaccg cgaccacgtc gccgcggcgc acgtgggcaa gcaacgtccc
     2221 gatcgaggtg tgcgcgggcg agatcgcaat gtcgatcggt ccgccctgca ccaggtcgac
     2281 atagctcttg cggttgatga gcgcgagcgt gcggcgcgcg cccatgcgct tggcgagcga
     2341 ggcggacatg atgttgtctt cctcgtcgtt ggtgagcgcg acgaagaggt cggtctcgtc
     2401 gatgccctcg gtctcgagca gcttctcgtc ggtggagtcg ccgcgcagca cgagcgcatt
     2461 cgacagatgc gaggcgagca tctcggcgcg ggtgcgattg agctcgagga tcttgacgtg
     2521 gtggttctcc tcgatcgact gcgcgagccg gaagccgatg ttgccgccgc ccgcgatcat
     2581 gatccgccgc atcggccggt cggagcggcg cagctcgcgc atgacctggc ggatgtgcac
     2641 cgtggcggcg aggcagaaca cctcgtcccc tggcaggatg atggtgtcgc cctcgggcat
     2701 gatcggttcg ccgttgcgat agatcgcggc gatgcggacc tcgacgttgg gcaggtggaa
     2761 gcgcagcgcc ttgagcggat ggccgaccag cggcccaccc tcgaaggcgc gcacgccgat
     2821 caggctcacc acgccgtcgg cgaactcgag cacctgcagc gcctcgggga aggcgatcag
     2881 gcgcttgatg tagtcggtga cgacctgctc gggacagatg gagaaatcca ccgcgaagtt
     2941 gtcgtcgtcg agcagttcgg gatggtcgac gtagtcggtc gagcgcaagc gcgcgatccg
     3001 cgtcggcagg ttgaacaacg ccttggcgat gcggcaggcg cacaggttgg tctggtcgga
     3061 ctgggtcacc gcgatgagca ggtcggcatc gtccgcgccg gcctcgcgca gcactcgcgg
     3121 cgaggcgccg ttgccgcaca cggtgcggat ctcgaggcgc tcgcgcagcg cgccgagata
     3181 ctcctcgctg gtgtcgacca gggtgatgtc gttcgcttcg gagacgaggt tctcggccac
     3241 cgaggcgccg acctggcctg cacccagaat gatgatcttc aacgttgtat ccggaagtgt
     3301 ccaagccgcc gattctacgc cccccacccg gcccggcaaa cgcagacgtt gcgccgcagc
     3361 aaccgccgga tcctgccgag ccgtggcgca gcggcaggag agcgcgcagg cttcaggact
     3421 cctcgtgccg gcgcccggcc tgcaggccga gttgcttgag cttgcggtag aggtgggtgc
     3481 gctccaggcc ggtcttctcc gccaggcggg tcatgttgcc gccctcgagg cgcagatgat
     3541 gttcgaagta catgcgctcg aaggcctcgc gcgcctctcg cagcggctgg tcgagcgaca
     3601 cgccgcaggc gccggcaccc agatccggtg gcagcaggcg acgcacttcg ccggcgccga
     3661 tctcctcctc cagcgtgccg agcgcgagcg acttcaccgc ggcgcgcaac tcggggtagc
     3721 cccccggcca cggcaggttg cgcagctggt tgagcgcggc ggtggaaaag cggcgcagcg
     3781 gcacctcgcc cgcctccacc agatgcagca gcatctggct ggcgagctcg ggcaggtcct
     3841 cgcggacgtc ggcgatcgag ggcggcgcga ggctcacctc gaacaggcgg gcgagcagcc
     3901 cctcctccca gccctcgccg accagggcct cgagactgcg gtcggtggcg acgacgaggc
     3961 gcaagtcgta gcgctcgagg cggtcgagcg cgaaggcgag gttcttttgc tgcgcgcgcg
     4021 acaggcgagc cagctcgccg acgaacacca cgccgccctg ggtggactgc aactgggtga
     4081 tgtcgagcgg cgcggtgacg gcggcgaggt cgagccaggg ggcgttggct ggttgcacgc
     4141 tgcgtgcgac cagttcggcg agcgaccccg cgccgacgcg cagcagcagc acgcgagaac
     4201 tgccgacgat ctgctcgagc cggcggcgca gctcgcgcaa gggcacggag cgatgaaagg
     4261 cggcgagcgt cggcgccgac tgcacgccgg gcgcctgcgg acgctgcagc ccgcgcttga
     4321 ccgcggcgag cagcttctgc agcgcgatcg gcttttccag atagtcgatc gcgccgatgc
     4381 gggtggcctc gaccgcggtg tcgatggtgc cgtgccccga catcatcacc accggcatgt
     4441 tgagctggcc attggccgcc cattccttga gcagggtgat gccgtcggtg tcgggcatcc
     4501 agatgtcgag caacaccatg tccggacgca ctgcgttgcg tgctgcgcgc gccgcggtgg
     4561 cattctcggc gagcaggatg tcgtgcccct cgtcgcggag gatctccgag agcagttcgc
     4621 ggatgccgat ttcgtcgtca acgatcagaa tctttgccat ttttcttcgt catgcttcct
     4681 tgttttcggc cagccgcagg cggatgcgca cttcggcgcc accgccctcg cggttgctga
     4741 ggcggacctc gccgccatgc tcgtcgacga tcttcttgac catcgccaag cccaggccgg
     4801 tcccgcgcga cttggtagtg aaatagggct cgaaggcgcg cgccaggact tcgggcggaa
     4861 aacccgggcc attgtcgcgg cacagcagca gcgcgctctc gccgtcacga cgggtgatca
     4921 ccgagatctc cgcgtcctcc tgcgcgccaa gcgcgtcctg cgcattttgc agcatgttgt
     4981 ggatcacctg ccggatctgc gtcggatcgc cctgcaccgg aggcagcgct ccccccagct
     5041 cggtgcggat ctgcaccgcg gcactctcgt acaggtggag cacctcgcgg atcagcgcgt
     5101 tgaggtcgag cggcgcgagc tggggcgccg gcagccgcgc atagtccctg aaggcgttga
     5161 ccaggttctt catcgcctcg acctgattca cgatggtggt ggttgcgcgc tcgagcatct
     5221 cacgcccgtt gtcgtcgagg cgatcggcga gcttgaagga gagacgctcg gccgaaagct
     5281 ggatcggcgt cagcgggttc ttgatctcgt gcgcgagccg gcgcgcgacc tcgccccacg
     5341 ccgccgtacg ctgcgcggcg atcaggctgg agatgtcgtc gaacaccacc accaagccgc
     5401 cccccgtgct ctcgggcagg cgcgagccgc gaatcagcag ggtgcgcggc gggcggtccc
     5461 tgaccggcaa ctccatctgc ttctgccagt ctcccgtgtt ggcggtgaag ccctcgcgca
     5521 gcatgtggcc aagctcggcc tggcgtggcc aggcctcgag cgggatgtcc tcgaatccgc
     5581 ccagatcatc ctcgaggatt tgcagcgcac catggttggc ggcgcgcagg tgaccgtcgg
     5641 ccgagaaggc gaggacgccg gtggacagat tcgccagcac cgactccagg taggcgcgcg
     5701 cagcctcgac ctcggcgcga ttgcggtcgg ccgccgcgcg cgcctcctgc agctggcgcg
     5761 tcatgcggtt gaaggactgc gtcagcacgc cgagttcgtc gttcgccggc aaggcctggc
     5821 gcgggctgaa atcgccctgc gccaccgcct gcgtgccctc ggccaggatc agcagcggtt
     5881 gcgccaatcg acgcgcgatc agcaccgcga ccgcgagcgc gccaagcagc gcgagcagca
     5941 gggtcaggct caaggtgacc gtgtagatgc gcttgagccc gctgcgtccg agcgtcagct
     6001 gctggtactc gcggtatgcc tcctggaccg cctcggcatt gcgaccgaag gattcgggga
     6061 ccggctgctg cagcatcagc agattggact cgcccgccag cgtgcgcacc gggatcggcg
     6121 tcagcacgcg gatcaccagg cgaccgtcgg gttcggcgat cacagaatga aaatggcggc
     6181 tctgacgggc ctgtcgcagt tcggccgtgc ccggaagctc gggaagcagc gtccgacccg
     6241 actcgaccgc ggtggcgagc acctggcccg cgggggcaag gatggtggcg ctcgcgatcc
     6301 cggcctgctc gcgcagccga ttcagccggg tacccgcgcc ctgcccggcc ccctcgagct
     6361 ccagcaccat gtcgtccgcc ttgtctcgca cctggctcac caggtaatcg agcgcgttct
     6421 ggccgagcgc gatcccgcct tcgagcgccg aatcgacccg cacgtcaaac caggattcga
     6481 tgctgcgcac cacgaactgc agggacacgc cgtagatcac cagccccggc accacgccca
     6541 tcagcgcgaa catcagcacc aggcgcatct tgagacggga accgaactgc ccctcgcggt
     6601 gctcgcgcag caggcgatgc agctgcatgc cgacgagcgc ggcgaggacc acggccatgc
     6661 cgccgttgag cgcgagcaac caaggatagc tggtcgcgaa cagggtggtg ttcgaactcg
     6721 ccgaggtgag caggaacaac gagatccccg acagcgctgc ggcgacggcg agcaggacgc
     6781 gcttcatcgg aaccccggcg cccccggcag gaaggtccag cgcacccact cggtctcgac
     6841 gttccagtcg ttgctgccga tcgcggtgac ctgaaagggc ttgggaagct ggctggtgtc
     6901 gagccggaag cgaagggaaa cctcgtgcga gaccccggac tggaggactc cggtctccgc
     6961 gacaagccag ttgcgcacgc gctgcatggt gcgcagcgcc tcgtcgagtt cgtcgaagtt
     7021 ctggtgcaga ttgccgaccg agagccggaa gctgcgcgtg atcgcgtggt aatagagccg
     7081 gtagtccagg ctacggcgcg ccaccgtctc gttgagccag taccagcgcg ggcgctcgat
     7141 cagcacctcg gtggtgaagt aaagcgagac gccgcgcgtg accacgtcga tcaggcgggg
     7201 attgagatcg atcgctatat ccgcgttgag gacataaccg ccgtcaccgg gcacgatctc
     7261 ggcgctgcgg atgacgctct ccgcgcgtac gctcagcgcc cacaacaaga gcagcgcggc
     7321 cacggcgaag cgaacggcgc gctcagggcc gcttgcggag gagcgcgtaa tagaagccgt
     7381 catggtcggc acaaggcagc agctgcaggt cggaagatcc gcgaatcggg agtcgctccg
     7441 cgtcggcatg gcggacgcag aagcgggcga cctgaccggc gttttcctcg tcgaacaccg
     7501 agcaggtgac gtagagcatt gtgccacccg gggcgagcgt gcgccaaagt gcctccagga
     7561 tttccgcctg ctgtgcggca aagttggcga tatcgctgtc ccggcgcaac cacttgatgt
     7621 ccggatgtcg gcgcaccacg ccggatgccg agcacggcac atcggccagg atgcggtcga
     7681 agggacgacc atcccaccac gctgccaggc ggcggcagtc ggcgaccttc agctccgcgc
     7741 gcaggccgag gcggtcgagg ttgcgcgcca cccgacccgc ccgcagcgga tcaagctcca
     7801 gcgcgagcag gtccgcatcg gccctctcga ggatgtgtgc agtcttgccg ccgggggctg
     7861 cgcaggcgtc gagcacacgc tcgccggctc gaacatcgag cagttgcgcc gcccactgtg
     7921 cccccgcgtc ctgcaccgac aagcgccctt cggcgtaccc gcgcaggcgt gcgaccgcca
     7981 gcggacgcgc gagcaccagc gcgtcgttgt cgagtcgccg gaactccagc ccggctgcgg
     8041 caagctccgc ctcgacctcg gccaacgtgg cacggcgcgg attgacccgc aaggccatgg
     8101 ggggacgcgt attgccggcc gcgagcagat cctgccaggc ctgaggatgc gcgctgcgca
     8161 cacgctccac ccaccaggcc ggaaaggcat gacgcgcctc gggctcggcc tcgatccagc
     8221 cttgccactc cggctgccga cgcagcgcat tgcgcagcac accgttgacc aggttgcgca
     8281 ggccgggcat cgccacggcc gtggcgccca cagcctgatc gaccaccgtg tgggcttgct
     8341 cggggcgctg cgtcaggcgc tgcagggcaa ccagcagaag cgcccgaatc tccaccggag
     8401 gaggactatg cagcaggcgg gaaaggatcg catcaccgcg cccgaactcg cgcaaggtcg
     8461 accaggcgag gtcgcgcacg gcgccacgcg tgccttccgg ccactccgga tgcccggcct
     8521 gcatgcgctc gaaggcatcg gtcaggttgg cgccgtcgat cacgccagcg accagttcgg
     8581 ttgcccgtgc gagcgcgtag ccgaggctgt cgatgggctg ttccggagca cggcgtgggg
     8641 caccacgcac accggctgtg cgggagcggg gcgagccggc ggccggcagg gcggaccgtc
     8701 ggtctttggg gtgggctggg gtcaaggtct ggagaacggc cgaagaagcg gcactggctc
     8761 aggccagcaa ggcacgcaga tgattgggca gggcgaactg cgcacagtgt accgcaccat
     8821 tgaagtgctg cagttcgcgg atggcgcgct cggcaagacg gcggtccacc tcgttcgcgt
     8881 cgagcgcagc cgggtcgagc gtatcggacg cgcaggccat gccccagagg ctcccataga
     8941 gcggaatgta gaggaagtac ggccgcaccc gcgcaaacac ccgacgcagg cgggcgagca
     9001 actctcgcac acgctcgggc tggaataccg gcgtaccgat atgcagcgtc agcgccccgc
     9061 cctcggccag cagggccttg caggctgcga agaagtcggc ggtgtagagc gcttccgccg
     9121 gcccgatcgg gtcggtcaga tcgagcacga tcaggtcgta gcgctcgccc gcggcgggag
     9181 catgctcgca cacatgggcc atcccgtccc cgatgcggat ctccacgcgg ggatcgtcga
     9241 gggcgccatg atgcacggca tcgaggtagc gccgggcgat gtcgaccacc ttcgcgtcga
     9301 gctcggcgat cacgatacgc tcgatgcccg gatacttgag cagctcttcg gccgaaccgc
     9361 catcccctcc cccgatgagc agcgcccggc gcaggccagg atgggcaacc ccgggaacgt
     9421 ggatcaggtt ctcatggtag tggaactcgt cgcgctcgga cgtcatgaaa cagccatcga
     9481 ggcgaaagag cttgccgaag cgcggcgttt cccagacctc gtagtcttgc cactcggagc
     9541 ggccggactc gagcaggcgc gcaccacgga gaaagtagcc ggcatcctcg ccgaggagct
     9601 cggcgaggac atccttcggc agatcggcaa accaggacgc tccgctcatg atcttctgca
     9661 ctccacagct ttcgatttca ggcagccacg accaccgccc gccgccaccc cggcggcgtc
     9721 aagccatcct tccatccaaa cacgaggtgc atccgcacgc gcggctgttc cacgtgaaac
     9781 agcccgtcag cgcaggaagg tatcgtaggc aaggcgaagg atcagcaccg ccaccactgc
     9841 aaggaacatc tttcgcacaa aacccgtacc gtgcctcagc gccatccggg aaccgaggat
     9901 cgagccgctc agattgcaca ccgccatgag cgccgccagc ttccacatca gctccacatt
     9961 gccgacaaag aaactgatcg ccgcgaggtt ggtcgccaca ttcacgatct tggccgaaac
    10021 cgatgcgcgg agaaagtcca tccccagcaa gcggatgaaa aggaagatca ggaagctccc
    10081 ggttccaggc ccgaagaagc catcgtagaa cccgatcact ccacccacgc cgagcccgat
    10141 cgcgaccgaa cgcgccccgt gctccggctc gcgcgagacc gcgccgagat ccttgcgcag
    10201 gaaggtgtac acggccacca acaccaacag tgccagaatc aacggacgca ggatcgcggg
    10261 atccaggtag gccaccgcct tggctccgta ccacgacccg acaagcgcag ccagggcacc
    10321 cggcgccgcg acgcgccacg gaatctcgac ccgtcggctg tactggacgg cggcactggt
    10381 cgtgccgacg atgctcgcga gcttgttggt accgaagagc gtcgcaggcg cggtcgtcgg
    10441 aaaggccgca aaaagtgcgg gcaactggat caagccgcca ccgcccacga tggagtccac
    10501 gaagccggca aacagcgcgg cgacgccgag ggcaaggaag aggaattcgg tttccatggg
    10561 aggggtgttt cacgtggaac agagagagac ggccgaggag gatcgcgacg gtttgacgca
    10621 acggcggccg acggcgcgca cgcgacaccg gatcccggca tgaacgccgg ctgtcgggcg
    10681 cgcctctgtt tgcgacttcc gccgcaccaa ccgaccgacg gtcagcccac ttactttccg
    10741 atgcagaagc gggcgaagat caccccgagc aggtcgtcgg cgctgaactc gccggtgatc
    10801 gtgccgagtt cctcctgcgc gaggcgcagc tcctcggcaa agagttcgag cgcaccgagt
    10861 tgcgtgcctg cagcctccac atggcggagc gcctcgcgca gcgcgagcag atgacgctcg
    10921 cgcgcaagaa tcacatcctc gccgtgcgca tgccagccgg cgatccgcaa cagctcctgg
    10981 cgcaccagct cgacaccctc cccgcttcgc gccgacacct gcaggcgaac ccgtccgccc
    11041 gcctccacgc ggcccgcggg caatcctgcg agatcgatct tgttcgccac cacgatacgc
    11101 tcgacccctt gcggcagacg ctcgtcgaga tccccctcgg acgccacccc ggcctcgacc
    11161 aggtgcagga tcacgtcggc gcgcgcgatc tcgcgccagg tgcgctcgat gccgatgcgc
    11221 tcgacccggt cctcggtctc gcgcaggccg gcggtgtcga tgatgtggag cgggataccc
    11281 tcgacctgga tcgtctcgcg cagcgcgtcg cgggtggtgc cggcgatgtc ggtgacgatc
    11341 gcgcgctcct cgccggcgag ctggttgagc aggctggact tgccgacgtt gggcgccccc
    11401 accagcacca cgtcgagccc gctgcgcagc agcgcaccct ggcgcgcgcg ctcgagcaca
    11461 ccctcgagcg cctcgcggat ggattcgagg cgcggcatcg cgcgcgcgcg ctcgaggaag
    11521 tcgatctctt cctcgggaaa atccagcgtt gcctcgacca gcatgcgcag gtcgatgagc
    11581 gcgtcgacga tgcgatggac ttcctcggag aactgcccgg acagcgagcg cacggccgag
    11641 cgcgcagccg ccgcggtcga ggcctcgatc aggtcggcga caccctcggc ctgagcgaga
    11701 tcgagcttgc cgttgagaaa ggcgcgacgg gtgaattcgc cgggctcggc caggcgggcg
    11761 ccgagttgca ggcagcgctc gagcagcatc tgcatgacca ccgggccgcc gtggccctgc
    11821 agctcgagca cgtcctcgcc cgtgaaggac gccggcgcgg cgaagtagag caggatcccc
    11881 tcgtcgatcg gccgctcgtg ctcgtcgagg aagcgggtga agtgcgcgat gcgcggctgc
    11941 ggcgtacggc cgcacagcgc gagcgcgaag gcgccgagac cggcacccga gacgcggacg
    12001 acaccgatgc cgccgcggcc cggtgccgtc gcgatcgcgg cgatcgtgtc aggcgggctt
    12061 gccggccgac tttccacttt cgatcatccg cgtgatctgc cactgctggg cgatcgacag
    12121 gcagttgttc accacccagt acagcaccag gccggacggg aaccacagga acatgaaggt
    12181 gaagatgatc ggcatcgcca tcatgacctt ggcctggatc ggatccggcg gcgtcgggtt
    12241 gagcttcatc tgcacgagca tggacacgcc catgatgatc ggcaggatga agtagggatc
    12301 cttggccgag aggtcctgga tccagcccag ccacggcgcg tggcgcatct cgacgctgcc
    12361 gagcagcacc cagtacaggg cgatgaacac cgggatctgc accaggatcg gcaggcagcc
    12421 gccgagcggg ttgatcttct cggtgcggta gagattcatc atctcctgct gcatcttggc
    12481 cttgtcgttg ccgtacatct ccttcatgcg ctgcaggcgc gggccgagca cgcgcatctt
    12541 ggccatcgac ttgtagctcg ccgccgacag cgggaagaag atcgccttga tcgcgacggt
    12601 gaccaggatg atcgcccagc cccagttgcc agtcaggttg tggaaccagg acagcaccca
    12661 gaacagcggc gccgcgatca cggtcagcca gccgtagtcg accaccaggt cgaggcccgg
    12721 ggcgatgcct tcgagcttgt cctgctcctg cgggccggca tacagacgat tggacaccgt
    12781 accctcggca cccggggcga tcgcggtcac cggcaggatc acgcccgccg agaacaggtt
    12841 gttgcccagc gcgcgcgcga agtactcgcg ctgcaggccc tgctccggca gccaggcgcc
    12901 gacgaagtag tgctgcacca tcgccaccca gccgtcgctg gccgtcttcg cgaacttggc
    12961 gctgccgtcg gtgatgtgct cgaaggtaac cttctggaac ttggccgcat cggtgtagaa
    13021 ggccgggccc gtgaaggtcc gcacgccgaa ggcctcgacc tgctcggccg gattgccgtc
    13081 gcgggtgagc tggaagtagg cgtgcggcgc gagcgcctgg cccgatccgt tgcggatctc
    13141 gtaggacacg tcgatcaggt agctgccgcg cgcgaagcgc atcaccttgg tgacctgcac
    13201 gccgttctgc tcgggcgcct gcagacgcag ctccacgccg ttctcgccgt ccttcaggcg
    13261 cagctcgcca gcgggcagct ggaacagggt cttgtgggtg ggcaggccct cgccgatcag
    13321 gcccgactgc gcggcgtact ggtgccgcgt ggtgccgtcg tcgagcagca cgaagttgcc
    13381 gcccgcctcg ccgctcgcct tgtgctgctt gagctcgagg cgaacgatgt caccgccctg
    13441 cgccgagacc tcggcacgca gcagatcggt ctcgaccacg gcacgcggcg cggccgcagc
    13501 ctgggcggcc tgctcgcccg gcaccaccgg ctggccgggc gtggcgctca acgtgggcgt
    13561 gggcaccgcg ccgtcggccg gcgccgcgct tccggcggcg gcctgctgct gggccacggg
    13621 aggctggttc tgcttgatcc agccgtccca cagcatgacg agcgagaagg agaaaatcag
    13681 aaagaggatg aggcggcgtt gatccatcgg aatcgttctg tctcaggtca gggaacggga
    13741 tcatacccgc ccggattgaa gggatgacag cgccccaccc gtttcgcggc catccagccg
    13801 ccacgcaacg caccgtggcg ctgcacggcc tcgatcgcgt actcggagca ggtcgggtgg
    13861 aagcggcaat tgcggccgag catcgggctg atcgcgtagc ggtagaaacg cagcagggcg
    13921 atcagcacgg tcttcattgc cggggcagcc tttgcagaag ggagagcagg tcgaggttga
    13981 gctcggcacg cgtggccttg gccaccggcg cgttcaggcg cacgaccaag tccatcgccg
    14041 gaagctcgat gcgctggcgc cggaagatct cgcgcgcgat gcgcttgacc aggttgcgca
    14101 cgttcgcgcg cttggccagc ttcttcgcca ccaccacgcc gaggcgggcg gtgtccagac
    14161 cattgggccg gtagtgcagc atgtagaacc ggccgcgcag cgcccgccga aaagcaaaaa
    14221 cggatgaata ctcatccgtt ttgtgcaatc tgtgagcgct gcggaaaccc tggtcagcgc
    14281 ccgtgggcga aaccacctca gacggcgagg cggtgacgac ccttggcacg gcgtgcgcgg
    14341 atgaccgcac ggccgccacg ggtcttcatg cggaccagga aaccatgggt gcgcttgcgg
    14401 cggacaacgg agggctgata agtgcgtttc atgatcggac tgccagtgtt aggtcaaacg
    14461 cgagagttaa atggataatg ccatgtttgt caataagaat ttttgcccga gcctgtggat
    14521 aagtctgcct ccgcagttta gaatgctgtc tttcgacgcc acgctcccat gcgctgctgc
    14581 gcggggcgag gcgcgcacag tgcgccgctc gagtgcaccg tcggtacaac tccccgactc
    14641 gtacagcaac ccggccgcgc ccccaggcgc cgcgcatcag gaatcccgct gcagcctgac
    14701 ccgccgcacg ctttcccgcc atcctgcccg acgacatcca agctcccgcc gtgactcaag
    14761 atttctggac cttctgcctg tcgcgccttg aacaggagct gccccagcag cagttcaaca
    14821 cctggatcaa gaccctgcgg gccgaacccg gcgagggtgg ctgggcgctc atcgcgccca
    14881 accgcttcgt gctgcaatgg gtgcgcgagc gctacctgcg ccgcgtcgag gaactcggcg
    14941 aagagttcgc cggcgccccg ctcgccatcg agttgcagct gccaccggcc ggcgccgcac
    15001 gcccggcgcg cgccgcagcc acggcggatg tcgctccggc gcgcgcgagc gcgccgaggt
    15061 cgacgatcga g
//
LOCUS       DAOQNN010000032        14886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1619_length_14886_cov_9.698132, whole genome shotgun sequence.
ACCESSION   DAOQNN010000032 DAOQNN010000000
VERSION     DAOQNN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1619_length_14886_cov_9.698132"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="PKV97_03650"
     CDS             <1..1346
                     /locus_tag="PKV97_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNB05000.1"
                     /translation="TLAWLGRLSQESVELAGRIRGDFTVGGTIESPRASGRIEGRELG
                     FTLVDQGLMLAGGELDVDFDQERVRLERLEFVSENRVRPRENRIPFERLTATPGRFTA
                     RGELALDSGAGDFRFDADRLPLLQRDDRWMLLSGGGTARSTFESLALDAEFRADAGYL
                     EFAEALPPSLSDDVVVLGREPAPAQQGGGFAVNADVSVGLGDALYLSALGLETRLAGG
                     LRIRLQPGRPLSAVGTIETVGGGYRGYGQNLTLERGLINFQGPLDTPGLNIVALRKGL
                     EVEAGVAITGSAKRPQIKLVSDPEVPDPTKLSWIVLGRAPDAGSGADLGLLLPAAAAL
                     LGGPGGGMTEELSRSLGFDSFSIGQGELNSTTRTRSSRVLGSGQTVSGGPSVAGQVLS
                     LGKRLGPDLFLNFEQGLGGAATLVKLTYQVSRRLSVIARGGTDTALDLQYGFSFR"
     gene            1425..1994
                     /gene="msrA"
                     /locus_tag="PKV97_03655"
     CDS             1425..1994
                     /gene="msrA"
                     /locus_tag="PKV97_03655"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNB05001.1"
                     /translation="MTESKNDPVRETAILGGGCFWCLEAVFDLVAGVDEVVPGYCGGH
                     VELPSYRDVCSGGTGHAEVVKIVFDPAKVSYRELLDVFFTIHDPTTVDRQGNDVGPQY
                     RSVIFATSEAQLHTALATIEALGERRAFPKAIVTRVERAPRFWPAEPEHHDYYVNNSD
                     QPYCQYVVAPKITKFRSHFDLRRWPEGSR"
     gene            2028..2372
                     /locus_tag="PKV97_03660"
     CDS             2028..2372
                     /locus_tag="PKV97_03660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNB05002.1"
                     /translation="MTQTTTASGLVIDELELGSGDTAEKGRMVSVHYTGWLTDGRKFD
                     SSKDRNDPFVFPLGAGHVIRGWDEGVQGMQVGGKRKLTIPPELGYGARGAGGVIPPNA
                     TLVFEVELLKVL"
     gene            2473..2931
                     /locus_tag="PKV97_03665"
     CDS             2473..2931
                     /locus_tag="PKV97_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004386182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNB05003.1"
                     /translation="MKLDPAQRHILTLLQKDSTLSTQALADKVGMSPTPCWRRVREME
                     EAGVIRGYVALVDRHKVGLGTCVWVRVKLKQHSADVLDRFERVVKACEQVVECYELLG
                     ETDCLLKLYLPNLEALSVFMHEFLLKIPEIDVTHSAVALREIKNETALPL"
     assembly_gap    3025..3124
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3212..4552)
                     /locus_tag="PKV97_03670"
     CDS             complement(3212..4552)
                     /locus_tag="PKV97_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNB05004.1"
                     /translation="MAKHAHSQWSSRMGFVLAATGSAVGLGNIWKFPYMVGQSGGAAF
                     VLVYLACIAFIGVPILVAEWMIGRRGQKNPINTMAQVARDNGHSTNWAVVGAIGVLAA
                     FLILSFYSVIGGWALAYMRDAATGAFIGLDKAAIGGAFEGFLARPAELLTWHSIFMLL
                     TVVVVALGVSAGLERGTKLMMPALGVILLVLVGYAMTTGSFGQGLAYLFNPDWSKLDG
                     KVLLAALGHAFFTLSLGMGIMMAYGSYLGQEVNLLRAARTVVIMDTVFALCAGMAIFP
                     IVFANGLDPAAGPGLVFVTLPLAFGHMGGGLVIGALFFLLLTFAALTSSISLLEPVVE
                     LIEERTPLGRVAATLIAGITIWALGIAALLSFNVWSDVKLLGMNIFDLLDYATSKFML
                     PLAGLGAIVFAAWKLDQQGVKAELGLGDATFGLWTLLSRYVAPVGVLFVFWSNL"
     gene            5028..8624
                     /locus_tag="PKV97_03675"
     CDS             5028..8624
                     /locus_tag="PKV97_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNB05005.1"
                     /translation="MSNIAPTVSLDDKYTLKTGRAWMTGIQALVRLPMMQKLRDEAAG
                     LNTAGFVTGYRGSPLGSVDQEFWKAKRFLEPMNIRFQPGLNEDLAATSVWGTQQLGLD
                     PNAKVDGVFSIWYGKGPGVDRTCDVFRHANAAGTSQHGGVLVIAGDDHAAKSSTLPHQ
                     TEHVFKALMMPVLAPAGIQEYLEYGLHGFAMSRYSGCWVAFKALTDTVETSSSVDIDP
                     FRVDIRVPSAQEFPLPLDGLNLRWPDPPLVQEERLLHHKLYAALAYGRKNGLDRMVID
                     PPQARLGILTSGKSYLDVRQALDDLGIDETTAAAIGLRLYKVGMVWPLDAEGVRRFAD
                     GLDEILVIEEKRQFLEYQLKEELYNWKDEVRPRVIGKFDEKGEWSLPHADWLLPAAGE
                     LTPAMIARAIAGRIARFHTSDRIKARLAFIEAKEAALARPRLSIQRVPHYCSGCPHNT
                     STKVPQGSRAIAGIGCHYMATWLSPEHTQTFCQMGGEGVPWIGQAPFTNTPHVFANLG
                     DGTYMHSGILAIRAAVAAKVNITYKVLYNDAVAMTGGQPHDGTLSVPIIARQLLAEGV
                     GTVVVVNDGTPRAYGPADLPHGVAVRHRDELDAVQRELRTAPAVTAIIYDQTCAAEKR
                     RRRKRGKFPDPAKRVVINDLVCEGCGDCSDKSNCMSVGAVETEFGRKRFIDQSSCNKD
                     YSCVKGFCPSFVTVEGGKLRRGKASGASALGGRLADELPEPQLPDTAAPWGILITGVG
                     GTGVVTIGALLGMAAHLEGKGISVLDMAGLAQKGGAVWSHVRIADRQEQLHAARVAAG
                     EANAVIGCDLVVAASDESLAKMQHGLTRVLINRDQTNTSEFVRGFAAQARSGDVLANP
                     DPQFPAAAMEAQIVDAVGADKAEFLDATALATELLGDSIATNLFMLGFAWQRGLVPLS
                     RQAIERAIEINGTAIEANKAAFLWGRRAAVDLRAVREAAKPRFGTPAHHRLSTSVDEL
                     IARRKTHLTDYQDAAYAERYLRLVERVRVAEAKVAPGITLLTEAVARGYHKLLAYKDE
                     YEVARLYTDSDFLARVEEQFEGDYKLVFHLAPPMLADKDPASGELKKKAYGPWMLKAM
                     RVLAKLRHLRGSRLDPFARSHDRKLDRELIADYERVVDEIVVNLDRANHEAAIELAAL
                     PEQIRGYGHVRERYLAGARKRQAALLEDFRHRRAPGTPLATKARKTIAIIPG"
     gene            9055..10104
                     /locus_tag="PKV97_03680"
     CDS             9055..10104
                     /locus_tag="PKV97_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNB05006.1"
                     /translation="MAVFSLSDFADHEQVVFVSDDKSGLKAIIAVHNSNLGPALGGCR
                     MWPYASEEEAVRDVLRLSRGMTYKSAMARLKLGGGKSVIIGNPRTDKTPALLAAFARA
                     LEQLNGRYIAAEDSGTSVADMKYMTQFTRHVAGIHDKPSDEGTRSGDPSPATAYGTFV
                     GIQTAVKERLGRDSLEGLRVAVQGVGNVGFDLARQLGEAGVKLWVTDIHREPLVQAGK
                     ELGATVVAPEEIFGLDVDVFAPCALGAILNDSTIPQLKAKIVAGAANNQLAEPRHGLE
                     LMKRGILYAPDYVINAGGIIDVYHERIGFERAALIRHIEGIRDNLMEVFERAREEERP
                     TAEVADAIAEERFRR"
     gene            10643..11740
                     /gene="coxB"
                     /locus_tag="PKV97_03685"
     CDS             10643..11740
                     /gene="coxB"
                     /locus_tag="PKV97_03685"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNB05007.1"
                     /translation="MLVAGVPAGAQDMRLNMTRGATELSRQVFDLHMTIFYICVAIAL
                     VVFGLMFWSIVRHRKARGAVPAQFHGSLKVEILWTAIPVLILVAMAVPATRTLIAMED
                     TSASELTVLVTGSQWKWHYKYLDYPIEFYSVLATPRAQIANAADKTPDYLRAVDKPLV
                     LPSGKKVRFLLTADDVIHSWWVPDFAIKKDAVPGFINETWARIDKPGLYYGKCAELCG
                     RDHGFMPVAVEVKTPADFEAWAAQQVAAAEQAKAEAESAGPMSMDEQMALGRQVYERA
                     CVACHQAGGEGLPGVFPALKGSAIATGEPAAHLHIVLFGKTGTAMQAFGKQLGVKELA
                     AVITYERNAWGNAKGDQVQPADIAAAIAKGE"
     gene            11744..13354
                     /gene="ctaD"
                     /locus_tag="PKV97_03690"
     CDS             11744..13354
                     /gene="ctaD"
                     /locus_tag="PKV97_03690"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HNB05008.1"
                     /translation="MSTQVTGTLDAHDAAGRPRDHDHKPRGLARWLFSTNHKDIGTLY
                     LLFSLAMLFTGGSLAMVIRAELFQPGLQFVDPHFFNQMTTVHGLVMVFGAVMPAFVGL
                     ANWMIPLMIGAPDMALPRINNWSFWILPCAFAILLSTLFMEGGAPAAGWTFYAPLSTK
                     YSGDSTAFFVLAVHLMGVSSIMGAINVIVTIWNMRAPGMGWMKLPLFVWTWLITAFLL
                     IAVMPVLAGVVTMVLTDKYFGTSFFDAAGGGDPVMFQHIFWFFGHPEVYIMILPAFGI
                     VSTIIPTFARKPLFGYESMVIATASIAFLSFIVWGHHMFTTGMPVVAELFFMYATMLI
                     AVPTGVKVFNWVATMWRGSMTFEVPMMFSLAFIVLFTIGGFSGLMLAIIPADFQYQDT
                     YFVVAHFHYVLVTGAVFGIIAAVYYWIPKWTGVMYDERLAQVHFWCSLVSVNMLFFPM
                     HFVGLAGMPRRIPDYALQFADLNAFMSIGGFLFGLSQLLFLWGVVRCMRGIGDKATDR
                     VWEGAQGLEWEVPSPAPYHTFDTPPVVK"
     gene            13383..13910
                     /locus_tag="PKV97_03695"
     CDS             13383..13910
                     /locus_tag="PKV97_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HNB05009.1"
                     /translation="MSAHRSLINKLLLACAAMFAFGFALVPLYDVFCDITGINGKTDK
                     VAAAAPDRVDRGREVSVEFLATHDPAVAVDFAPETARLRLNPGELQLVSFVVENRSDQ
                     PIVTTAVPSVSPGEAARHFMKIECFCFQEQPLAPGERKLMPVQFYVDPELPERFKNLT
                     LSYRLYRSVGQRASR"
     gene            13957..14841
                     /locus_tag="PKV97_03700"
     CDS             13957..14841
                     /locus_tag="PKV97_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNB05010.1"
                     /translation="MATTTQQKYYVPHDSAWPIVGALALLLIGYGAATWISQLDQPEA
                     RSGPWVFGAGFALLVVTLFGWFGKVIDESQRGLYSAQLERSFRQCMSWFIFSEVMFFL
                     AFFGALFYARVVAVPWLDGAGDNAMTAAILWPKFEAAWPLLQTPGGTATQAMPWTGIP
                     LLNTLILLASSVTLQVAHVAIEQGKRLRLKLFLSITVLLGVVFLFLQGYEYMHAYRDL
                     GLRLDSGVYGNTFYLLTGFHGLHVTIGALILLVMLLRIVIKDHFTAQRHFGFQAAAWY
                     WHFVDVVWLNLFVFVYVL"
BASE COUNT         2332 a         5192 c         4985 g         2277 t
ORIGIN      
        1 cgacgctcgc ctggctcggg cgcctctcac aggagagcgt cgagctcgcc gggcgcatcc
       61 ggggcgactt caccgtgggc ggcacgatcg agtcgccgcg tgcgagcggc cgcatcgagg
      121 ggcgcgagct cggcttcacc ctcgtcgacc agggcctgat gctcgccggt ggcgagctcg
      181 atgtcgactt cgaccaggag cgcgtgcgcc tggagcggct cgagttcgtc tccgagaacc
      241 gcgtgcgtcc gcgcgagaat cgcatcccct tcgagcgcct caccgccacg cccgggcgct
      301 tcaccgcgcg cggcgagctt gcgctcgaca gcggcgcggg cgatttccgc ttcgacgccg
      361 accgcctgcc gctgctgcag cgcgacgacc gctggatgct gctgtcgggc ggcggcacgg
      421 cgcgcagcac cttcgagtcg ctggcgctgg acgcggagtt ccgcgccgac gccggctacc
      481 tcgagttcgc cgaggcgctg ccgcccagcc tctccgacga cgtcgtcgtg ctcgggcgtg
      541 agcccgcacc ggcgcagcag ggcggcggct tcgcggtgaa tgcggatgtc agcgtcggcc
      601 tcggcgacgc gctctacctc tcggcgctcg gcctcgagac gcggctcgcc ggcgggctgc
      661 gcatccgcct gcagccgggg cggccgctgt cggcggtggg cacgatcgag accgtgggcg
      721 gcggctaccg cggctatggg cagaacctca ccctggaacg cggcctgatc aacttccagg
      781 gcccgctcga cacgcccgga ctcaacatcg tcgcgctgcg caagggcctg gaggtggagg
      841 ccggggtggc gatcaccggc agcgccaagc gcccgcagat caagctggtg tccgatcccg
      901 aggtgcccga ccccaccaag ctgtcgtgga tcgtgctcgg gcgcgcgccg gacgccggct
      961 ccggcgccga cctcggcctg ctgcttccgg ccgccgcggc cctgctcggc gggccgggtg
     1021 gcggcatgac cgaggagctc tcgcgcagcc tcggcttcga ctccttctcg atcggccagg
     1081 gcgagctcaa cagcaccacg cgcacgcgca gcagccgggt gctcggcagc ggtcagacgg
     1141 tctcgggcgg gccctcggtc gccggccagg tgctgagcct gggcaagcgc ctcgggccgg
     1201 accttttcct caacttcgag cagggcctgg gcggggccgc caccctggtc aagctgacct
     1261 accaggtctc gcgccgcctg tcggtgatcg cccgcggcgg caccgacacc gccctcgacc
     1321 tccagtacgg cttctccttc cgttgaggcg cgtggcgggc ggggaatgat tcccctcccg
     1381 tgcggtctct ccgatgccat gaccaacccc gatggagcga acagatgaca gagagcaaga
     1441 acgatccggt acgcgagacc gccatcctgg gcgggggctg tttctggtgc ctggaggcgg
     1501 tgttcgacct ggtggccggc gtggatgagg tcgtgcccgg ctattgtggc ggccacgtcg
     1561 agctgccgag ttaccgcgac gtgtgctccg gcggcaccgg tcacgccgaa gtggtgaaga
     1621 tcgtgttcga tccggcgaag gtgagctacc gtgagctgct cgacgtcttc ttcaccatcc
     1681 acgatcccac cacggtcgac cgccagggca acgacgtggg cccgcagtac cgctcggtga
     1741 tcttcgccac ctccgaggcg cagctgcaca ccgcgctcgc caccatcgag gcgctcggcg
     1801 agcgccgcgc gttcccgaag gcgatcgtca cccgggtcga gcgtgcgccg cgcttctggc
     1861 cggccgagcc ggagcaccac gactactatg tgaacaatag cgaccagccg tattgccagt
     1921 acgtggtggc ccccaagatc acgaaattcc gcagccactt cgacctgcgc cgctggcccg
     1981 aaggcagcag gtagaatgcg cgctttcact cacggcagga gaacggcatg acccagacca
     2041 ccaccgccag cgggctcgtc atcgatgaac tcgagctcgg cagcggcgat accgccgaga
     2101 agggccgcat ggtgtcggtg cattacaccg gctggctcac cgacgggcgc aagttcgact
     2161 cgagcaagga tcgcaacgat cccttcgtct tcccgctcgg cgccggccac gtgatccgcg
     2221 gctgggacga gggcgtgcag ggcatgcagg tgggcggcaa gcgcaagctc accatcccgc
     2281 ccgagctcgg ctacggcgcg cgcggcgccg gcggcgtgat cccgcccaac gccaccctgg
     2341 tgttcgaggt cgaactgctc aaggtgctgt gatccctcgc gggggcgggc gcccccgccg
     2401 cgaggcggcg cggccgcctc gccatcgaca ccaggagaac ccggcgtttc gagccgggca
     2461 aggcagggag acatgaaact cgacccggcg cagcgtcaca tcctgaccct gctgcaaaag
     2521 gactccaccc tcagcacgca ggcgctcgcc gacaaggtcg gcatgtcgcc gacgccgtgt
     2581 tggcggcgtg tgcgcgagat ggaggaggcg ggcgtgatcc gcggctacgt cgcgctggtc
     2641 gaccgccaca aggtgggcct gggcacctgc gtgtgggtgc gcgtcaagct caagcagcac
     2701 agcgccgacg tgctcgatcg cttcgagcgc gtggtcaagg cctgcgagca ggtggtggag
     2761 tgctacgagc tgctcggcga gaccgactgc ctgctcaagc tctacctgcc gaacctcgag
     2821 gccttgtcgg tgttcatgca cgaattcctg ctcaagatcc ccgagatcga cgtcacccat
     2881 tccgcggtgg ccctgcgcga gatcaagaac gagaccgccc tgccgctgtg aggcgggcag
     2941 ggcggtcgcg gtgtcggcac gatgcaggct tcgcgccgcg cttgctggag cgccggatcg
     3001 cgactcgcgt cgctcctacg ggcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnatcgcg actcgcgtcg ctcctacggg cggggccgga cgcgccgcga ttttgtagga
     3181 gcgacgcaag tcgcgatcgg tgcgtcggtt cctacaggtt gctccagaac acgaacagca
     3241 cgcccaccgg cgcgacgtag cgcgacagca gggtccacaa gccgaatgtg gcatcgccaa
     3301 ggcccagttc cgccttcacg ccctgctggt ccagcttcca cgccgcgaac acgattgcac
     3361 ccaggccggc gagcggcagc atgaacttgc tggtcgcata gtcgagcagg tcgaagatgt
     3421 tcatgccgag cagcttgacg tcgctccaca cgttgaagga cagcagcgcg gcgatgccca
     3481 gcgcccagat ggtgatgccg gcgatcagcg tggcggcgac gcggccgagc ggggtgcgct
     3541 cctcgatcag ctccaccacc ggctcgagca gcgagatcga cgaggtcagc gcggcgaagg
     3601 tcagcagcag gaagaacagt gcgccgatga ccaggccgcc gcccatgtgg ccgaaggcca
     3661 gcggcagggt cacgaacacc aggccggggc cggccgcggg gtccaggccg ttggcgaaca
     3721 cgatcgggaa gatcgccatg ccggcgcaca gcgcgaacac cgtgtccatg atcaccacgg
     3781 tgcgcgcggc gcgcagcagg ttcacctcct gcccgaggta ggagccgtag gccatcatga
     3841 tgcccatgcc cagcgacagg gtgaagaagg cgtggccgag cgcggcgagc agcaccttgc
     3901 cgtcgagctt gctccagtcc gggttgaaga ggtaggcgag gccctggccg aagctgccgg
     3961 tggtcatcgc gtagccgacc agcaccagca ggatcacccc gagcgccggc atcatcagct
     4021 tggtgccgcg ctccaggccg gcggacacgc cgagcgccac gacgacgacg gtgagcagca
     4081 tgaagatcga gtgccaggtc agcagctcgg ccgggcgggc gaggaagccc tcgaaggcgc
     4141 cgccgatcgc cgccttgtcc aggccgatga aggcgccggt ggcggcgtcg cgcatgtagg
     4201 ccagcgccca gccaccgatc accgagtaga aggacaggat caggaaggcg gcgagcacgc
     4261 cgatcgcgcc caccacggcc cagttggtgc tgtggccgtt gtcgcgcgcg acctgggcca
     4321 tggtgttgat cgggttcttc tgcccgcgcc ggccgatcat ccactccgcg accaggatgg
     4381 gcacgccgat gaaggcgatg caggccaggt agaccagcac gaaggcggcg ccgccgctct
     4441 ggccgaccat gtagggaaac ttccagatgt tgccgaggcc gacggcggag ccggtggcgg
     4501 cgagcacgaa gcccatccgc gacgaccatt ggctgtgtgc gtgcttggcc atgtcagcgt
     4561 tccctgcgga gagtgggtgc gacgggggcg tgcggccgcg cgggggcggc gccgcgggtg
     4621 gcggcgttcg ggttcatgca ggtctcctcg gatcgtttca cgctcgtggc gtgtggaggg
     4681 aaaaggcgcg gattctaaac gtccgaggcc tgccgcccaa gcggggatgc ctgcgccgcg
     4741 acgtgtgcgg atccgtgccg accccttttg tggtcgccgg acggttgggt ccggatggcg
     4801 cgcatcgtac gacttgatga gtgaaataat ttttcttata tcggcgtccg cagccccgat
     4861 tgcgcaaaat cttttccgcc gctcgcccta gcatgtgcat ccacgagcgc cgcaggccga
     4921 gccggccgcg gttgcgcacc gacccacccg cagaggagac cacgccgacc cgccggcagc
     4981 ccgcgccaga cgcgcaggcc tgccgaccga cggcggttca cgccaccatg tcgaacatcg
     5041 cccccaccgt cagcctcgac gataaataca ccctcaagac cggccgcgcc tggatgaccg
     5101 gcatccaggc cctggtacgc ctgccgatga tgcagaagct gcgcgacgag gccgccggcc
     5161 tgaacaccgc cggcttcgtc accggctacc gcggctcgcc gctcggcagc gtcgaccagg
     5221 agttctggaa ggcgaagcgc ttcctcgagc cgatgaacat ccgcttccag cccgggctca
     5281 acgaggacct cgccgcgacc tcggtgtggg gcacgcagca gctcggcctc gaccccaacg
     5341 ccaaggtcga tggcgtgttc tccatctggt acggcaaggg acccggcgtg gaccggacct
     5401 gcgacgtctt ccgccacgcc aacgccgccg gcacctcgca gcatggcggc gtgctggtga
     5461 tcgccggcga cgaccacgcc gccaagtcct cgaccctgcc gcaccagacc gagcacgtgt
     5521 tcaaggcgct gatgatgccg gtgctcgccc ccgccggcat ccaggaatac ctggaatacg
     5581 gcctgcacgg cttcgcgatg tcgcgctact cgggctgctg ggtggcgttc aaggcgctga
     5641 ccgacacggt ggagacctcg tcctcggtcg acatcgatcc cttccgcgtg gacatccggg
     5701 tcccgagcgc gcaggagttc ccgctgccgc tcgacggcct caacctgcgc tggcccgacc
     5761 ccccgctggt gcaggaggag cgcctgctcc accacaagct ctacgccgcg ctcgcctacg
     5821 gccgcaagaa cgggctggac cgcatggtca tcgacccgcc gcaggcgcgc ctgggcatcc
     5881 tcaccagcgg caagagctac ctcgacgtgc gccaggcgct cgacgacctc ggcatcgacg
     5941 agaccaccgc cgcggcgatc ggcctgcgcc tgtacaaggt cggcatggtg tggccgctcg
     6001 acgccgaggg cgtacgccgc ttcgccgacg gcctcgacga gatcctcgtg atcgaggaga
     6061 agcgccagtt cctcgagtac cagctgaagg aagagctcta taactggaaa gatgaggtgc
     6121 gcccgcgcgt gatcggcaag ttcgacgaga agggcgagtg gagcctgccg cacgccgact
     6181 ggctgctgcc cgccgccggc gagctcaccc cggcgatgat cgcgcgcgcg atcgccggcc
     6241 gcatcgcgcg cttccacacc tccgaccgca tcaaggcgcg gctcgccttc atcgaggcca
     6301 aggaggccgc gctcgccagg ccgcgcctgt ccatccagcg cgtgccgcat tactgttccg
     6361 gctgcccgca caacacctcg accaaggtgc cgcagggctc gcgcgcgatt gccggcatcg
     6421 gctgccacta catggcgacc tggctgtcgc ccgagcacac gcagaccttc tgccagatgg
     6481 gtggggaggg cgtgccctgg atcggccagg cgccgttcac caacaccccg cacgtgttcg
     6541 ccaacctcgg cgacggcacc tacatgcact cgggcatcct cgccatccgc gccgcggtgg
     6601 cggcgaaggt gaacatcacc tacaaggtgc tctacaacga cgcggtggcg atgaccggcg
     6661 gccagcccca tgacggcacg ctgtcggtgc cgatcatcgc ccgccagctg ctcgccgagg
     6721 gcgtgggcac ggtggtggtg gtcaacgacg gcacgccgcg cgcctacggc ccggccgatc
     6781 tgccgcacgg cgtggcggtg cgccaccgcg acgagctcga tgccgtgcag cgcgagctgc
     6841 gcaccgcgcc ggccgtcacc gcgatcatct acgaccagac ctgcgccgcc gagaagcgcc
     6901 gccgccgcaa gcgcggcaag ttccccgatc ccgccaagcg cgtggtgatc aacgacctcg
     6961 tgtgcgaggg ctgcggcgac tgcagcgaca agtcgaactg catgtcggtg ggcgcggtcg
     7021 agaccgagtt cggccgcaag cgcttcatcg accagtcctc gtgcaacaag gactattcct
     7081 gcgtgaaggg cttctgcccg agcttcgtca ccgtcgaggg cggcaagctt cggcgtggca
     7141 aggcgagcgg cgcgagcgcc ctcggcggcc gtctcgcgga cgagctcccc gagccgcagc
     7201 tgcccgacac cgccgcgccc tggggcatcc tcatcaccgg tgtcggcggc accggcgtgg
     7261 tgaccatcgg cgccctgctc ggcatggccg cgcacctgga aggcaagggc atctcggtgc
     7321 tcgacatggc ggggctggcg cagaagggcg gcgcggtgtg gtcgcacgtg cgcatcgccg
     7381 accgccagga gcagctccac gccgcgcgcg tcgccgccgg cgaggccaac gccgtgatcg
     7441 gctgcgacct cgtggtggcg gccagcgacg agtcgctcgc caagatgcag catgggctca
     7501 cccgcgtcct catcaaccgc gaccagacca acaccagcga gttcgtgcgc ggcttcgccg
     7561 cccaggcgcg cagcggcgat gtgctcgcca accccgaccc gcagttcccg gcggcggcga
     7621 tggaggcaca gatcgtcgac gcggtcggtg cggacaaggc cgagtttctc gacgccaccg
     7681 cgctcgccac cgagctgctc ggcgattcga tcgccaccaa cctgttcatg ctcggcttcg
     7741 cctggcagcg cggcctggtg ccgctctcac ggcaggcgat cgagcgcgcg atcgagatca
     7801 acggcaccgc gatcgaggcc aacaaggccg ccttcctgtg gggccggcgc gccgcggtcg
     7861 acctgcgtgc ggtgcgtgag gccgccaagc cgcgcttcgg cacgcccgcg caccaccgcc
     7921 tgtcgaccag cgtcgacgag ctgatcgcgc gtcgcaagac ccatctgacc gactaccagg
     7981 atgccgccta cgccgagcgc tacctgcgcc tggtcgagcg cgtgcgggtc gcggaggcga
     8041 aggtcgcgcc cggcatcacc ctgctgaccg aggcggtggc gcgcggctac cacaagctgc
     8101 tcgcctacaa ggacgagtac gaggtcgcgc gcctgtatac ggacagcgac tttctcgcgc
     8161 gcgtcgagga gcagttcgag ggcgactaca agctcgtctt ccacctcgcg ccgccgatgc
     8221 tggccgacaa ggacccagcc agcggcgagc tgaagaaaaa ggcttacggt ccctggatgc
     8281 tgaaggcgat gcgtgtgctg gcgaagctcc gccacctgcg cggatcgagg ctcgacccct
     8341 tcgcccgcag ccacgaccgc aagctcgacc gcgagctgat cgccgactac gagcgtgtgg
     8401 tcgacgagat cgtcgtcaac ctcgaccgcg ccaaccacga ggccgcgatc gagctggcgg
     8461 ccctccccga gcagatccgc ggctacggcc acgtgcgcga gcgctacctc gccggcgcac
     8521 gcaagcgcca ggccgcgctg ctggaggact tccgccaccg ccgcgcaccg ggcacgccgc
     8581 tcgccaccaa ggcgcgcaag accatcgcga tcatcccggg ctgaggcacg ccgccgtaat
     8641 cgcgacttgc gtcgctccta cgaagatcgc cgcgctggcg caggggctgc gggagcgacg
     8701 ccagtcgcga tcgacgatcc cgaatcgagc gcggtgcaga ccggcagcgc cgcaatcgcg
     8761 actggcgtcg ctcctacgga aaaccggcgc gctggcggag gcgctgtggg agcgacgcaa
     8821 gtcgcgatcg acgatcgcga atcgagcgcg gtacagaccg gcagcgccgc aatcgcgact
     8881 ggcgtcgctc ctacggaaaa ccggcgcgct ggcggaggcg ctgtgggagc gacgcaagtc
     8941 gcgatcgacg gcgaaccgga acccctcttc acccctgccg cagcgccggc gtcgccaccc
     9001 ctggcgccgg gactgcggaa atcatccacg ttcccacaag gaaggagaca cagcatggcc
     9061 gtattttccc tgagcgactt cgccgaccac gagcaggtgg tcttcgtcag cgacgacaag
     9121 agcggactga aggcgatcat cgccgtccac aattccaacc tcggccccgc gctcggcggc
     9181 tgccgcatgt ggccctatgc cagcgaggag gaggcggtgc gcgacgtgct gcgcctgtcg
     9241 cgcggcatga cctacaagtc ggcgatggcc aggctcaagc tcggtggcgg caagtcggtg
     9301 atcatcggca acccgcgtac cgacaagacg cccgcgctgc tcgccgcctt cgcgcgcgcg
     9361 ctcgagcagc tgaacggccg ctacatcgcc gccgaggact ccggcaccag cgttgccgac
     9421 atgaagtaca tgacgcagtt cacccgtcac gtcgccggca tccacgacaa gccttcggac
     9481 gagggcacgc gcagcggcga cccgtcgccg gccaccgcct acggcacctt cgtcggcatc
     9541 cagacggcgg tgaaggagcg tctcggccgc gactcgctcg aaggcctgcg cgtggccgtg
     9601 cagggcgtgg gcaacgtcgg ctttgacctc gcccgccagc tcggggaggc gggcgtgaaa
     9661 ttgtgggtca ccgacattca ccgcgagccg ctggtgcagg cgggcaagga actgggtgcc
     9721 accgtggtcg cgccggagga gatcttcggg ctcgacgtcg atgtgttcgc gccctgcgcg
     9781 ctcggcgcca tcctgaacga ttcgaccatc ccgcagctga aggcgaagat cgtcgccggt
     9841 gccgccaaca accagctcgc cgagccccgc cacggcctcg agctgatgaa gcgcggcatc
     9901 ctctatgcgc ccgactacgt catcaacgcc ggcggcatca tcgacgtgta tcacgagcgc
     9961 atcggcttcg agcgcgccgc actgatccgc cacatcgagg ggatccgcga caacctcatg
    10021 gaagtcttcg agcgcgcgcg cgaggaggaa cgtcccaccg ccgaggtcgc cgacgcgatc
    10081 gccgaggagc gcttccggcg ctgagccgcg ggcgtcgcgc ggggcggttg cacggcacgg
    10141 cacgtccacc atgccccgag cgaaggcacg atcgaaggct cggaggcgtt cggatcgcgc
    10201 ccgggcacgg caaagaacgc ctccgggcat tcggccatgc ccgaggctcg gtgcagcccc
    10261 cagcggcccg gccgtgcgcg tccctgtgct gcgcgcgcga ggcatcgtct tgcgcttgcc
    10321 gatgcgcctg catgcagtgc gaatgcgaat ggctcgcgct gccgttcgca tccatccttg
    10381 cctttcgtat tttcccgcat atattgacgt aagagttccg cccgtcgtga tggcggccgc
    10441 ccaggcaagg cctggcggcc cgggtccgtt ccccaggacc gcgcaccacg ccatgacgcc
    10501 gactcgggac ggcggacctt cgtgcacgca ttcgcggcgg tttcacgccc ataacgataa
    10561 aggggggtcg gcagtgagac accattcatc cggggcgagg cccgggacgc tccctcccgg
    10621 ccgtgccctg gtcgcggcct cgatgctggt ggccggcgtt cctgcgggcg cgcaggacat
    10681 gcgcctgaac atgacgcgcg gcgccaccga gctcagccgg caggtcttcg acctgcacat
    10741 gaccatcttc tacatctgcg tcgccatcgc gctggtcgtg ttcggcctga tgttctggtc
    10801 catcgtgcgt caccgcaagg ccaggggggc agtgccggcg cagttccacg gcagcctgaa
    10861 ggtcgagatc ctgtggaccg cgatcccggt gctgatcctc gtggcgatgg cggttccggc
    10921 cacccgcacg ctgatcgcga tggaagacac ttccgcatcc gagctcaccg tgctggtgac
    10981 cggttcgcag tggaagtggc actacaagta cctcgactac ccgatcgagt tctactcggt
    11041 gctcgccacg ccgcgcgcgc agatcgccaa cgcggcggac aagacgccgg actacctgag
    11101 ggcggtggac aagccgctgg tgctgcccag cggcaagaag gtgcgcttcc tgctgaccgc
    11161 cgacgacgtc atccactcgt ggtgggtacc cgacttcgcg atcaagaagg acgcggtgcc
    11221 cggcttcatc aacgagacct gggcgcgcat cgacaagccc ggcttgtact acggcaagtg
    11281 cgccgaactg tgcgggcgcg accacggctt catgccggtg gcggtcgagg tgaagacgcc
    11341 cgcggacttc gaggcctggg cggcgcagca ggtggccgcc gccgagcagg ccaaggccga
    11401 ggccgagagc gccggaccga tgagcatgga cgaacagatg gcgctcggcc ggcaggtcta
    11461 tgagcgggcc tgtgtcgcct gccaccaggc cggcggcgag ggcctgcccg gcgtgttccc
    11521 ggcattgaag ggcagcgcga tcgccaccgg cgagccggcg gcccacctcc acatcgtgct
    11581 gttcggcaag accggcaccg cgatgcaggc tttcggcaag cagctcggtg tcaaggagct
    11641 ggccgcggtc atcacgtacg agcgcaacgc ctggggcaac gccaagggcg accaggtgca
    11701 gccggccgac atcgcggccg cgatcgcgaa gggggagtga gtcatgagca cccaggtcac
    11761 cggcaccctc gatgcacacg acgctgcagg gcgtccgcgc gaccacgacc acaagccgcg
    11821 cggcctcgcg cgctggctgt tcagcaccaa ccacaaggac atcggcacgc tctacctgct
    11881 gttctcgctc gccatgctgt ttaccggcgg cagcctggcg atggtgatcc gcgccgagct
    11941 cttccagccc gggctgcagt tcgtcgatcc gcacttcttc aaccagatga cgacggtgca
    12001 cggcctggtg atggtgttcg gcgcggtgat gccggccttc gtcggcctgg cgaactggat
    12061 gatcccgctg atgatcggcg cgcccgacat ggcgctgccg cggatcaaca actggagctt
    12121 ctggatcctg ccgtgcgcgt tcgccatcct gctgtccacg ctgttcatgg aaggcggcgc
    12181 gccggccgcc ggatggacct tctacgcgcc gctgtcgacc aagtacagcg gagattcgac
    12241 cgccttcttc gtgctcgcgg tgcacctgat gggggtgtcc tcgatcatgg gggcgatcaa
    12301 cgtcatcgtc accatctgga acatgcgcgc gccgggcatg ggctggatga agctgccgct
    12361 gttcgtgtgg acctggctga tcaccgcctt cctgctcatc gcggtgatgc cggtgctggc
    12421 cggcgtggtc accatggtgc tgaccgacaa gtacttcggc accagcttct tcgacgccgc
    12481 gggcggcggc gacccggtga tgttccagca catcttctgg ttcttcggcc atccggaggt
    12541 gtacatcatg atcctcccgg ccttcggcat cgtctccacc atcatcccca ccttcgcgcg
    12601 caagccgctg ttcggctacg agtcgatggt gatcgccact gccagcatcg ccttcctgtc
    12661 cttcatcgtg tggggtcacc acatgttcac caccggcatg ccggtggtcg ccgagctgtt
    12721 cttcatgtat gccaccatgc tgatcgccgt gcccaccgga gtgaaggtgt tcaactgggt
    12781 ggcgacgatg tggcgcggct cgatgacttt cgaggtgccg atgatgttct cgctcgcctt
    12841 catcgtgctg ttcaccatcg gcggcttctc cgggctgatg ctggccatca tccccgccga
    12901 cttccagtac caggacacct acttcgtcgt cgcccacttc cactacgtgc tggtgaccgg
    12961 cgcggtgttc ggcatcatcg ccgcggtgta ctactggatc ccgaagtgga ccggcgtgat
    13021 gtacgacgag cgcctcgccc aggtgcactt ctggtgctcg ctggtgtcgg tgaacatgct
    13081 gttcttcccg atgcacttcg tcggcctcgc cggcatgccg cggcgcattc ccgactacgc
    13141 gctgcagttc gccgacctca acgccttcat gagcatcggc ggcttcctgt tcggcctgtc
    13201 gcagctgctc ttcctgtggg gcgtggtgcg ctgcatgcgc ggcatcggcg acaaggccac
    13261 cgatcgcgtg tgggagggcg cacaggggct ggagtgggag gtgccgtcgc ccgcgcctta
    13321 ccacaccttc gacactccgc cggtggtcaa gtgagcgcac ggcggcacag ggaggacgcg
    13381 agatgtctgc ccaccgtagt ttgatcaaca agctgctgct ggcctgtgcg gcgatgttcg
    13441 ccttcggctt cgcgctcgtg ccgctgtacg acgtgttctg cgacatcacc ggcatcaacg
    13501 gcaagaccga caaggtggcg gcggcggccc cggaccgcgt cgaccgcggg cgcgaggtca
    13561 gcgtcgagtt cctagccacc cacgaccccg cggtggcggt ggacttcgcg cccgagaccg
    13621 cgcgcctgcg gctcaacccg ggggagctgc agctcgtgtc cttcgtggtc gagaaccgca
    13681 gcgaccagcc gatcgtgacc accgccgtac cctcggtgtc gcccggcgag gccgcccgcc
    13741 acttcatgaa gatcgaatgc ttctgcttcc aggagcagcc gctggcgccg ggcgagcgca
    13801 agctcatgcc ggtgcagttc tacgtcgatc cggaactgcc cgagcgtttc aagaacctga
    13861 cgctgtcgta tcgcctgtat cgcagcgtcg gccagcgcgc gagccgctga cgcggcgcgg
    13921 cccgggcgcg cgcgaccgca gcaagggaga agcaccatgg cgacgaccac gcagcagaag
    13981 tactacgtcc cgcacgacag cgcctggccg atcgtcgggg cgctcgccct gctgctgatc
    14041 ggctacggcg cggcgacctg gatcagccag ctcgaccagc ccgaggcgcg tagcggcccc
    14101 tgggtgttcg gcgctggctt cgcgctgctg gtggtgacgc tgttcggctg gttcggcaag
    14161 gtcatcgacg aatcgcagcg cgggctctac agcgcgcagc tcgagcgttc cttccgccag
    14221 tgcatgagct ggttcatctt ctccgaggtc atgttcttcc tcgccttctt cggcgcgctg
    14281 ttctatgcgc gcgtggtggc ggtgccgtgg ctggacggtg ccggcgacaa cgcgatgacc
    14341 gccgcgatcc tgtggccgaa gttcgaggcc gcctggccgc tgctccagac ccccggcggc
    14401 accgcgaccc aggccatgcc atggaccggc attccgctgc tcaacaccct gatcctgctc
    14461 gcctcgtcgg tgacgctgca ggtggcgcac gtggcgatcg agcagggcaa gcgcctgcgc
    14521 ctgaagctct tcctgtcgat caccgtgctg ctcggcgtgg tgttcctgtt cctgcagggc
    14581 tacgagtaca tgcacgccta tcgggatctc ggcctgcgcc tggattcggg cgtgtatggc
    14641 aacaccttct acctgctcac cggcttccac ggcctgcacg tgaccatcgg cgcgctgatc
    14701 ctgctcgtga tgctgctgcg catcgtgatc aaggaccact tcaccgccca gcgccacttc
    14761 ggttttcagg ccgcggcgtg gtactggcat ttcgtggacg tggtgtggct caacctcttc
    14821 gtcttcgtct atgtgctgtg aggcccggcc atgcggactc agtacggacg tgcatgcggc
    14881 gtgatc
//
LOCUS       DAOQNN010000033        14469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1702_length_14469_cov_11.741709, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000033 DAOQNN010000000
VERSION     DAOQNN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 14469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14469
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1702_length_14469_cov_11.741709"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PKV97_03705"
     CDS             <1..203
                     /locus_tag="PKV97_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258716.1"
                     /note="CcoN; FixN; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C oxidase Cbb3"
                     /protein_id="HNB05011.1"
                     /translation="ATNPDGTLTYSFVESVKASYPFWTIRFIGGAMFLGGMLIMFYNM
                     VKTIAGQKAYDAPVLAPAAAHA"
     gene            219..854
                     /gene="ccoO"
                     /locus_tag="PKV97_03710"
     CDS             219..854
                     /gene="ccoO"
                     /locus_tag="PKV97_03710"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNB05012.1"
                     /translation="MAQSKHEKIERNVFLMIVLTLMTVSVGGLVEIVPLFFQHSTTTA
                     IDHKGNALDVKPYDPVRLVGRDIYVREGCYNCHSQMIRPFRAETERYGHYSVAGEYIY
                     DHPFQWGSKRTGPDLARVGGRYSDEWQRVHLLNPRDVVPESNMPAFPWLDRPVRAADI
                     QDRMRALNKVGLHKYSDEEIAAAPAALEGKTELDAVVAYLQGLGTAISHFR"
     gene            870..1058
                     /locus_tag="PKV97_03715"
     CDS             870..1058
                     /locus_tag="PKV97_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HNB05013.1"
                     /translation="MDINDFRSLITVLGLLCFLGICAWAYSKQAKAGFDEAARLPLSD
                     DDLPAPRGRQDQEGKTNG"
     gene            1051..1941
                     /gene="ccoP"
                     /locus_tag="PKV97_03720"
     CDS             1051..1941
                     /gene="ccoP"
                     /locus_tag="PKV97_03720"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HNB05014.1"
                     /translation="MADFISGFWNMYVMVLVALSILFCVFVLASNMTKREKGPVQLHG
                     HVWDETLAEYNNPLPRWWMYLFWITIVFAVVYLAIYPGFGRSNQSRGQWAQYDAEMQA
                     AQERFGPVFAKYREMDVMAVAADPEANAMGKRLFLTYCQQCHGAAAQGAKSFPNLTDS
                     EWLWGGAPENIKETITNGRNGVMPPFGPALGADGVKDVANYVRSMNGLAHDSVRAQRG
                     KEAFESNCVACHGADAKGNPMLGAPNLTDNRWLYGSSEAVIIETVTNGRNNKMPAFGA
                     FLGEDKVHLLAAYVLSLSKK"
     gene            2045..3478
                     /gene="ccoG"
                     /locus_tag="PKV97_03725"
     CDS             2045..3478
                     /gene="ccoG"
                     /locus_tag="PKV97_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNB05015.1"
                     /translation="MKSASPEPQKAARPSNPESSQDTLYAARKKLYVRSVTGLFANWR
                     WALVWITQILFYGLPWLSWNDRQAVLFHLTERKFYIFGWVFWPQDVFFLAILLIISAY
                     ALFFFTAIAGRLWCGYACPQTVYTEIFMWIEEKIEGDHTRRQKLDRAPMSSGKALRRA
                     VKFLVWGIVALWTGFTFVAYFSPLDELLASFNGLTFGPWETFWILFYAGFTYLFAGVL
                     REQVCKYMCPYARFQAVMFDPDTLVITYDEARGEPRGTRKKGIDPRSVSKGDCIDCGI
                     CVQVCPTGIDIRDGLQYECIGCAACIDACDQVMDKMNYPRGLIRYSTENAMKRNWGSK
                     EILAHVFRPRTLIYGTILAAICAAFVWGLAAREPLRVDVLRDRSSLGQEIEGGLIENV
                     YQLQVMNMTESPRAFSIGVSGLNGIRIKGPQRVEVAPAGVQSVTVQVHVPYDEGKPGA
                     NTIFFDVAAEDDPALTLKEESTFLVPR"
     gene            3480..3989
                     /locus_tag="PKV97_03730"
     CDS             3480..3989
                     /locus_tag="PKV97_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNB05016.1"
                     /translation="MRSKKPEAPVEPWYRQGWPWFLIALPATAVVAGIATLVIAAKTF
                     DGMVVDDYYKEGQAIVQTLGRVEHARELGLRAALRIRSDSIHVELTATAPGSLPDSIR
                     LTVAHPTRSGLDQELLVRGRSGVYEATLAPLSTGRWLFVIQDEARSWRMDGSAYLPAE
                     MEIRMDPNV"
     gene            4014..4163
                     /locus_tag="PKV97_03735"
     CDS             4014..4163
                     /locus_tag="PKV97_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HNB05017.1"
                     /translation="MAWELLFKSDIGLLSLFTIVFILVMAVYLYRFARRHMAEDEQKA
                     RSARS"
     gene            complement(4245..4688)
                     /locus_tag="PKV97_03740"
     CDS             complement(4245..4688)
                     /locus_tag="PKV97_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB05018.1"
                     /translation="MFKHILVPTDGSALSDSTVARAVSFAKETGARITFFYSQPDFPM
                     PIYGEGALIDPTTPEQFAKSAASEAEAILSKARGAAEAAGVACDSDTAVNEVPYEAII
                     DAADRHGCDLIFMASHGRRGIAGLLLGSETQKVLTHSKTPVLVYR"
     gene            4824..6233
                     /locus_tag="PKV97_03745"
     CDS             4824..6233
                     /locus_tag="PKV97_03745"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB05019.1"
                     /translation="MMLTFLGAAGEVTGSCLRVDHERGAFLVDCGLFQGGREADRKNR
                     DALDFELRDIDFVLLTHAHLDHSGLLPRLVALGYRGRIFATTATVDLLGVMLMDSAHI
                     QEKEAEWALRDTRSRKMARRAEVAPLYTVEQARTSLGRFVPTDYDDWIEPGPGVRCRF
                     RDAGHILGSAILEIDVGERGHTRRLVASGDIGQPMRPVLRDPVRIARADYLCVESTYG
                     NRAHRSFAATCDELALALRRTLEVDGGNVIIPAFAVGRTQEVLFVLADLVRAGRITRL
                     DVVVDSPMASAVTALTVRHAALWDDETRALYAWAEANSGRFRVRFVQDVEESKALNAV
                     PGGLVVISASGMCDAGRIKHHLRHNLGRRECAVVIAGFQAAGTLGRRLVDGAHQVTLF
                     GERIPVRARIHTIGGLSAHADQPALLDWLRGFGEAPRRTFVAHGEPAASAAFVEAVER
                     ELGWSRVEAARPGVPVVLE"
     gene            complement(6275..6958)
                     /locus_tag="PKV97_03750"
     CDS             complement(6275..6958)
                     /locus_tag="PKV97_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB05020.1"
                     /translation="MPNTTPLDIPSLLRRIPLFSELSEADIQLVARYTRERQVARGEV
                     LFQRGDLPHGFYFVVSGQVKLAFSSSQGTEKVVEIIGPMQSFGEAVMFMNHPYPVFAE
                     ALSETVLVHVGQAVVSELIDQDSTFARKLLAGMAIRLHGLIQDVETYSLRSSTQRVIG
                     YLLQVADNDAPCEIALPTSKQVIASRLNLTPETLSRIFHDLSDAGLITVQGKRVSLHD
                     PVRLSRYNA"
     gene            7041..7421
                     /locus_tag="PKV97_03755"
     CDS             7041..7421
                     /locus_tag="PKV97_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HNB05021.1"
                     /translation="MYYLIKNLHVTCVVLSAAGFLLRGTWMLTGNALLQHRLTRLLPH
                     LVDSTLLLSAITLAVMIGQYPFSAGWVTAKVIGLLAYILLGTLALKRGRTRGVRLAAF
                     AGAILVYAWIVSVALSKNVAGYLA"
     gene            complement(7458..8198)
                     /gene="fnr"
                     /locus_tag="PKV97_03760"
     CDS             complement(7458..8198)
                     /gene="fnr"
                     /locus_tag="PKV97_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HNB05022.1"
                     /translation="MRASVPITVDGLKSACSQCNLVELCLPFGMSDRELSRLDELVGA
                     RRKVKRQHNLYRAGDSFEAIYAIRAGSFKTDVLLEDGREQVTGFQMTGEILGLDGIST
                     EAHSCNAVALEDSEVCVIAYDKLEQMSHEIEGLQLQFHKVMSREIVRDHGVMMLLGSM
                     RAEERLAAFLLNMSQRFTARGFSPSEFNLRMTREEIGSYLGLKLETVSRAFSRFQEDG
                     LIAVQQKHVHLLDLAGLKRLIQHQHGAR"
     gene            8339..9730
                     /gene="hemN"
                     /locus_tag="PKV97_03765"
     CDS             8339..9730
                     /gene="hemN"
                     /locus_tag="PKV97_03765"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNB05023.1"
                     /translation="MKSQTDLIFDPQLIRKFDINGPRYTSYPTADRFVEAFNAEALRA
                     WLAKRAVGGVSKPLSLYFHIPFCNTICYYCACNKIITKDHGRSAKYLKYLAKEIEMQA
                     ACLEGSRQVTQLHLGGGTPTFLSHDEMRELMAAVREHFTLVPNGEYSIEVDPRKVDFD
                     TVKLLAELGFNRMSVGVQDFAEDVQQAVNRIQSFDETKLVIDAARANGFKSVSMDLIY
                     GLPRQNPTSFDRTLDQVLEISPDRISLYSYAHLPGLFKPQRRINSGDMPTADTKLALL
                     QLGIRRLTEAGYVYIGMDHFAKPDDELTIAQRQGRLHRNFQGYSTHAECDLLAFGVSA
                     IGKIGPVYAQNVKTLDEYYDALDRDELPVLRGIELTADDLLRRAIIQALMCHFELSMQ
                     SIEIAYLVNFREYFAEELADLQEMEKAGLVKIDGDWISVQPGGRLLVRGIAMVFDRYL
                     RADRERQRYSRVI"
     gene            9802..10512
                     /locus_tag="PKV97_03770"
     CDS             9802..10512
                     /locus_tag="PKV97_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB05024.1"
                     /translation="MPETGYLAVFLIGLLGGTHCVSMCGGIVGALSMQVQVPGSRPQW
                     PLHLAYNLGRIGTYTLLGAGVGLLGSVGLLYEGMLPVQMTLYVLANLMLVALGLYLTG
                     FTRLLAPIERVGHLLWRRIQPATRRFLPARSVGQALPLGMLWGFIPCGLTYSILATAL
                     VTGSGARGAGLMLAFGLGTLPNLLLAGMLFKRFRDIARHPRVRLASGLLVLGFGLFGL
                     YHAPSLGGDLWRGVVCVT"
     gene            10559..10768
                     /locus_tag="PKV97_03775"
     CDS             10559..10768
                     /locus_tag="PKV97_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNB05025.1"
                     /translation="MNETTIKVEGMSCGGCVKNVTGVLKALPGVEDVRVSLEGASATV
                     RHDPAKVSIDALRAAVEGAGFDSPA"
     gene            10859..11386
                     /gene="ppa"
                     /locus_tag="PKV97_03780"
     CDS             10859..11386
                     /gene="ppa"
                     /locus_tag="PKV97_03780"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNB05026.1"
                     /translation="MGFEQVSAGKDVPNDINVIIEISAQGDPIKFEVDKDSGAVFVDR
                     FMGTSMRYPLNYGYVPHTIAGDGDPVDVLVVTPFPLQPGVVIRCRPVGVLKMEDDGGV
                     DAKVVAVPVSKLTPLYDKVQTTDDLPELLMKQTVHFFEHYKDLEPGKWVKVLGWGTVE
                     DAKAEIVNGLAAAKK"
     assembly_gap    11463..11562
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11563..12548
                     /locus_tag="PKV97_03785"
     CDS             <11563..12548
                     /locus_tag="PKV97_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HNB05027.1"
                     /translation="MLALLMKINKNLILAIPVAMVLGFGFGLLAPVGWLKSLIVPLTF
                     LMVYPMMVNLKLSKVLEGGDLKAQALAQAINFALIPFVAYALGKAFFPDQPAYALGLL
                     LAALLPTSGMTISWTGFAKGNLGAAVKMTVLGLILGSLATPLYVRWLMGAEVPVDMGA
                     VSMQIVVIVFLPMLAGHYTQKWLVARHGQAEYQKEWGPRFPPFSTLGVLGIVFVALAL
                     KAKDLVAQPGELIVVLVPLLCLYAINYVVSTVVARSLLPRGDAIALVYGTVMRNLSIA
                     LALAMNVFGASSSGAALVIALAYILQVQSAAWYVKLTDRFFGPAAPAKAGG"
     gene            complement(12675..14093)
                     /gene="pyk"
                     /locus_tag="PKV97_03790"
     CDS             complement(12675..14093)
                     /gene="pyk"
                     /locus_tag="PKV97_03790"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNB05028.1"
                     /translation="MKRERSARILATLGPASSTRERIRALAEAGADVFRLNFSHGSHE
                     DHAERYRLIRAVEAEIGRPIGVLMDLQGPKLRVGRFAEGKVALVPGARFRLDLDPTAG
                     DAKRANLPHPEIFAALEAGTELLLDDGKLRLRVDACGADFADTTVLVGGTLSDRKGVN
                     VPGVVLPISPLTTKDRADLDFGLALGVDWVALSFVQRPEDLREARELIGDRAWIMAKL
                     EKPAAIDALDAIIALADGVMVARGDLGVELPPQQVPVLQRRIVRAARAAGRPVVVATQ
                     MLESMISSPVPTRAEASDVATAIYDGADAVMLSAESASGQYPVEAVAIMHGIVCEVER
                     DPAWRAGLEASHTAAAANTPDAICCALRRVAGLLEPAATVAYTSSGFSALRASRERPV
                     APILALTPQPTTARRLALVWGVHAVPFEEVHDVAEMVEHAARAVQNLGFGSAGDAVVV
                     IAGLPFGQRGSTNLLHVARIPG"
     gene            complement(14111..>14469)
                     /locus_tag="PKV97_03795"
     CDS             complement(14111..>14469)
                     /locus_tag="PKV97_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNB05029.1"
                     /translation="NGWAVSIAICDAGGHALWLQRMDGAPLMSAIVAPEKARTCVLSG
                     KPSKAFEDMVNNGRFAALAMPVVPLEGGEPVVVDGRVLGAVGVSGVKAGEDARVARAG
                     VDALLAHALPHPRSAA"
BASE COUNT         2311 a         4868 c         4750 g         2440 t
ORIGIN      
        1 gcgcgaccaa cccggacggc acgctgacct actccttcgt cgagtcggtc aaggccagct
       61 acccgttctg gacgatccgc ttcatcggcg gcgcgatgtt cctcggcggc atgctgatca
      121 tgttctacaa catggtgaag acgatcgccg gccagaaggc ctatgacgcc ccggtgctcg
      181 cgcccgcggc tgcccacgcc tgatcggaga acaacaacat ggctcaatcg aaacacgaaa
      241 agatcgaacg caacgtattc ctcatgatcg tgcttacgct gatgaccgtc agcgtggggg
      301 gtctggtgga gatcgtgccg ctgttcttcc agcactcgac caccacggcg atcgaccaca
      361 agggcaatgc gctcgacgtc aagccgtacg atccggtacg tctcgtgggg cgcgacatct
      421 acgtccgcga aggctgctac aactgccact cgcagatgat ccgtccgttc cgcgccgaga
      481 ccgagcgcta cggccactat tcggtcgccg gcgagtacat ctacgaccac ccgttccagt
      541 ggggctccaa acgcaccggt ccggacctcg cccgcgtcgg tggccgctac tccgacgaat
      601 ggcagcgtgt gcacctgctc aatcctcgcg acgtcgtgcc cgagtcgaac atgccagcct
      661 tcccctggct cgatcgcccg gtcagggcgg cggacatcca ggaccgcatg cgcgcgctca
      721 acaaggtcgg tctgcacaag tacagcgacg aggagatcgc cgccgcgccg gccgcgctcg
      781 agggcaagac cgaactcgac gccgtcgtgg cctacctcca ggggttgggc accgcgattt
      841 cgcacttcag gtaaggaagc actcgcgccg tggatatcaa cgatttccgt agcctgatca
      901 ccgtgctggg gctgctctgc ttcctgggta tctgtgcctg ggcgtacagc aagcaggcga
      961 aggccggttt cgacgaggcg gcgcgtctgc ccctgagcga cgacgaccta ccggccccgc
     1021 gcggccggca agaccaagag ggaaaaacaa atggctgact tcatcagcgg tttctggaac
     1081 atgtatgtga tggtcctcgt ggccctctcc atcctgttct gtgtgttcgt gctggcgtcg
     1141 aacatgacca agcgcgagaa agggccggtg caattgcatg gccatgtgtg ggatgaaacg
     1201 cttgcggagt acaacaaccc cctgccgcgg tggtggatgt acctgttctg gatcaccatc
     1261 gtgttcgcgg tggtttacct ggcgatctat cccggctttg gccgcagcaa ccagagccgc
     1321 ggtcagtggg cgcagtacga tgcggagatg caggccgccc aggagcgttt cggtcccgtg
     1381 ttcgccaagt atcgcgagat ggacgtgatg gcggtcgccg ccgatcccga agccaacgcg
     1441 atgggcaagc gcctgttcct gacctactgc cagcagtgcc acggcgcggc ggcgcagggt
     1501 gcgaagagct tccccaacct gaccgacagc gagtggctgt ggggcggtgc gccggaaaac
     1561 atcaaggaga ccatcacgaa cgggcgtaac ggcgtgatgc ctccgttcgg tcccgcgctc
     1621 ggcgccgacg gtgtcaagga tgtcgccaac tacgtgcgtt cgatgaacgg cctcgcccac
     1681 gactccgtgc gcgcgcagcg cggcaaggaa gccttcgagt ccaactgcgt ggcttgccac
     1741 ggtgcggatg ccaagggcaa cccgatgctc ggcgcgccga acctgaccga caaccgctgg
     1801 ctgtacggtt cgtccgaggc ggtgatcatc gagaccgtca ccaacggccg caacaacaag
     1861 atgcccgcct tcggtgcctt cctcggcgag gacaaggtgc acctgctcgc cgcctacgtg
     1921 ctgagcctga gcaagaagta agcgtcaccc gcagtagctg cagcagcatc gcatccccgg
     1981 gaggaagggg ccctcccggg gttctcgata acaacaagaa caagaattca tcggtaacaa
     2041 ggccatgaaa agcgcatccc ccgagccgca gaaggcggca agaccctcca accccgagtc
     2101 ctcccaggac acgctctacg cggcacgcaa gaagctctac gtgcgctccg tcaccggcct
     2161 gttcgccaac tggcggtggg cgttggtgtg gatcacccag atcttgttct acggtctgcc
     2221 ctggctgagc tggaacgacc gccaggccgt gcttttccat ctgaccgaac gcaagttcta
     2281 catcttcggc tgggtcttct ggccgcagga cgtgttcttt ctcgcgatcc tgttgatcat
     2341 ctcggcctac gccctgttct tcttcaccgc gatcgcgggc cgcctgtggt gcggatatgc
     2401 ctgcccgcag acggtgtata ccgagatctt catgtggatc gaggagaaga tcgagggcga
     2461 ccatacccgc cgccagaagc tcgaccgcgc gccgatgagc tccggcaagg cgctgcgccg
     2521 cgccgtcaag ttcctcgtct ggggcatcgt cgcgctgtgg accggcttca ccttcgtggc
     2581 ctatttctcc ccgctcgacg aattgctggc ctccttcaac ggcctcacct tcgggccctg
     2641 ggaaacgttc tggatcctgt tctacgccgg cttcacctac ctcttcgccg gcgtgctgcg
     2701 cgagcaggtg tgcaagtaca tgtgcccgta cgcgcgcttc caggcggtga tgttcgaccc
     2761 cgacaccctg gtgatcacct acgacgaggc acgtggcgag ccgcgcggaa cgcgcaagaa
     2821 gggcatcgat ccgcgctccg tgtccaaggg tgactgcatc gactgcggca tctgcgtgca
     2881 ggtttgcccg accgggatcg acatccgcga tggcctgcag tacgaatgca tcggctgtgc
     2941 cgcctgcatc gacgcctgcg accaggtcat ggacaagatg aactacccgc gcggcctgat
     3001 ccgctactcg accgagaacg cgatgaagcg caattggggc tccaaggaga tcctcgcgca
     3061 cgtgttccgg ccgcgcacgc tgatctacgg caccatcctg gccgcgatct gcgctgcctt
     3121 cgtgtggggc ctggctgcgc gcgaacctct gcgcgtcgac gtgctgcgcg accgctcctc
     3181 gctcggacag gagatcgagg gtggcctgat cgagaacgtg taccagctcc aggtcatgaa
     3241 catgaccgag tcgccgcgtg cgttcagcat cggcgtctcc ggcctgaacg gaatccgcat
     3301 caagggtccg cagcgggtcg aggtcgcgcc ggccggcgtg cagtcggtca ccgtacaggt
     3361 gcacgtcccc tacgacgaag gcaagccggg ggccaacacg attttcttcg acgtcgctgc
     3421 ggaggatgat cccgccttga cgctcaagga agagtccact ttcctggtac cgcgctgaca
     3481 tgcgaagcaa gaagcccgaa gccccggtcg agccctggta ccgccagggc tggccctggt
     3541 tcctcatcgc cctgcccgcg accgcggtgg tcgccggcat cgccaccctg gtgatcgccg
     3601 cgaagacctt cgacggcatg gtggtcgacg actactacaa ggaaggccag gcgatcgtgc
     3661 agaccctcgg ccgcgtcgag cacgcccgcg agctcggctt gcgcgccgcc ttgcgcatcc
     3721 gcagcgactc gatccacgtg gagctgacgg ctaccgcgcc gggcagcctg cccgacagca
     3781 tccgcctgac cgtcgcccac cccacgcgga gtggcctcga ccaggagctg ctcgtccgcg
     3841 ggcgctccgg tgtgtatgag gccacgctcg caccgctgag caccggtcgc tggttgttcg
     3901 tgatacaaga cgaggcccga agctggagaa tggacgggtc cgcttacctc cctgcagaga
     3961 tggagatccg gatggatccg aatgtctgag tttcttgcaa agggagaagc accatggcct
     4021 gggaattgtt gttcaagagc gatatcggtc tgctgagcct cttcaccatc gtcttcattc
     4081 tggtgatggc cgtctacctt taccgcttcg cccgccgcca tatggcggag gacgagcaga
     4141 aggcccgtag cgcccgctca tgagcgatcc gcaccgcgga tgaaaacggc ccgcacgagc
     4201 gggccgcatg ctttgggggg ccccgcgcgg ggccgatgcc ggtgtcagcg gtagaccaga
     4261 accggcgtct tgctgtgggt cagcaccttt tgcgtctcgg agccgagcag caggcctgcg
     4321 atgccgcgac ggccgtgcga ggccatgaag atgaggtcgc agccatggcg gtcggcggcg
     4381 tcgatgatcg cctcgtaggg cacctcattg accgcggtgt cgctgtcgca ggccacgccc
     4441 gccgcttccg ccgcaccgcg cgcctttgaa aggatcgcct cggcttcgga tgcagccgat
     4501 ttcgcgaact gctcgggcgt ggtcggatcg atcagcgcgc cctcgccgta gatcggcatc
     4561 gggaagtccg gctgcgaata gaagaaggtg atgcgcgcgc cggtctcctt cgcgaaggag
     4621 acggcacggg cgaccgtgct gtccgagagt gccgagccgt cggtcggtac caggatgtgc
     4681 ttgaacatga cgtacgctcc ctgtggtgtg tcgtgcttcg attatagcga gcggatgggg
     4741 tccgccatga ttgatcatga tcaagccgtc gagcgatcac gaagggcaca gtgggaaccc
     4801 ctgaaagatc ggcgaggcca gcgatgatgc tcaccttcct tggcgctgcg ggcgaggtca
     4861 ccggctcctg cctgcgggtc gatcacgagc gcggtgcatt cctggtggat tgcggccttt
     4921 tccagggcgg gcgcgaagcc gaccgcaaga accgcgacgc cctcgatttc gagctgcgcg
     4981 acatcgattt cgtgctcctc acccacgccc atctcgatca ctccggcctg ctgccgcgcc
     5041 tcgtggcact gggatatcga gggcgcatct tcgccaccac ggccactgtc gatctgctcg
     5101 gcgtgatgtt gatggattcc gcccatatcc aggagaagga ggccgagtgg gcgctgcgcg
     5161 acacgcgctc gcgcaagatg gcacgccggg cggaggtggc cccgctctac accgtggagc
     5221 aggcgcgtac cagtctgggc cgtttcgtcc ccaccgacta cgacgactgg atcgagcccg
     5281 gcccgggcgt gcgctgccgg ttccgtgacg ccgggcacat cctcggttcg gcgatcctcg
     5341 agatcgacgt cggcgagcgc ggccacaccc gccggctggt cgcctcgggc gacattggcc
     5401 agccgatgcg cccggtgctg cgggacccgg tgcggatcgc ccgggccgac tacctgtgcg
     5461 tcgagtcgac ctacggcaac cgcgcccacc gctccttcgc cgccacctgc gacgagctcg
     5521 cgctcgcatt gcggcgcacg ctcgaggtcg atggcggcaa cgtgatcatc ccggcgttcg
     5581 cggtcgggcg tacccaggaa gtgctgttcg tgctcgccga cctggtgcgc gccggacgca
     5641 tcacccggct cgacgtcgtg gtcgactcgc cgatggcctc tgcggtcacc gcgctcaccg
     5701 tgcgccacgc cgccctctgg gacgatgaga cgcgtgcgct ctacgcctgg gccgaggcca
     5761 actcgggacg cttccgtgtg cggttcgtgc aggacgtcga ggaatcgaag gcgctcaatg
     5821 cggtgccggg cgggctggtc gtgatctcgg caagcggcat gtgcgatgcc ggtcggatca
     5881 agcaccacct gcgccacaac ctcggccggc gcgagtgcgc agtggtcatc gcaggcttcc
     5941 aggctgccgg cacgctcggc cgccggctgg tcgacggtgc gcaccaggtc accctgttcg
     6001 gcgaacgtat cccggtacgt gcgcgcatcc ataccatcgg cgggctctcc gcccacgccg
     6061 accagcccgc actcctcgac tggctgcgcg gcttcggcga ggcgccgcgg cgcaccttcg
     6121 tggcgcacgg cgagccggct gcctccgccg ccttcgtcga ggcggtcgag cgcgaactgg
     6181 gctggagcag ggtcgaggcg gcgcggcccg gcgtgccggt cgtgctcgag tgagcgtccg
     6241 cgcgcaagcg gaccgagagg gcggcgaggg ccgctcaggc gttgtagcgc gacagccgta
     6301 ccggatcgtg cagactcaca cgcttgcctt gcacggtgat caggccggcg tcgctgaggt
     6361 cgtggaagat gcgcgacagg gtctcgggcg tcaggttgag gcgcgaggcg atcacctgct
     6421 tgctcgtcgg cagcgcgatc tcgcagggtg cgtcgttatc ggccacctgc agcaggtagc
     6481 cgatcacgcg ctgggtgctc gagcgcaagg agtaggtctc cacgtcctgg atcagcccgt
     6541 gcaggcggat cgccatgccg gcgagcagct tgcgtgcgaa ggtggagtcc tggtcgatga
     6601 gttcgctcac caccgcctgg ccgacgtgca ccagcacggt ctcgctcagg gcctcggcga
     6661 agaccgggta ggggtggttc atgaacatca cggcctcgcc gaagctctgc atcgggccga
     6721 tgatctcgac caccttctcg gtgccttgcg aggacgagaa ggcgagcttg acctggccgg
     6781 agaccacgaa gtaaaagccg tgcggcaagt cgccgcgctg gaacagcact tcgccgcggg
     6841 cgacctggcg ctcgcgggtg tagcgcgcga ccagctggat gtccgcctcc gacagttcgc
     6901 tgaacagcgg gatgcggcgc agcagggagg ggatgtcgag cggggtcgtg ttgggcatct
     6961 ggaactctcc gtctggggac gcaatttcaa ttcggccggt tgtcgagcgg cgccctgacc
     7021 ggctttcgtt aggatcgtgg atgtactacc tgatcaagaa cctgcatgtg acctgcgtcg
     7081 tgctgagcgc cgccggcttc ctgctgcgcg gcacctggat gctcaccggc aacgccctgc
     7141 tgcagcaccg cctgacgcgg ctcctgccgc acctcgtcga cagcacgctg ctgctgtctg
     7201 cgatcacgct ggcggtaatg atcgggcaat accccttcag cgccggctgg gtcacggcga
     7261 aggtgatcgg cctgctcgcc tacatcctgc tcggcacgtt ggcgctcaag cgcggacgta
     7321 cccgtggcgt gcggctcgcc gccttcgccg gtgcgatcct ggtctatgcg tggatcgtgt
     7381 cggtggcgct gagcaagaac gtcgccggct acctcgcctg agccgtcgag ccccgggtcg
     7441 tcccggccac ggcgtgctca gcgcgcgccg tgctggtgct gaatcaggcg cttgaggccg
     7501 gcgaggtcaa gcaggtgtac gtgcttctgc tgcaccgcga tcagcccgtc ctcctggaag
     7561 cgcgagaagg cgcgcgacac cgtctcgagc ttgagtccga ggtaggagcc gatctcctcg
     7621 cgcgtcatgc gcaggttgaa ctcgctcggc gagaaaccgc gcgccgtgaa gcgctgcgac
     7681 atgttcagca ggaaggcggc caggcgctct tcggcgcgca tcgagccgag cagcatcatc
     7741 acgccgtgat cgcgcacgat ctcgcggctc atgaccttgt ggaactgaag ttgcaagccc
     7801 tcgatctcat gcgacatctg ctcgagcttg tcgtaggcga tcacgcaaac ctcgctgtcc
     7861 tcgagcgcca ccgcgttgca gctgtgggcc tcggtgctga tgccgtcgag accgaggatc
     7921 tcgccggtca tctggaagcc ggtcacctgt tcgcgaccgt cctcgagcag cacgtcggtc
     7981 ttgaacgagc ccgcgcggat cgcgtagatc gcctcgaaag agtcaccggc gcggtacaga
     8041 ttgtgctggc gcttgacctt gcggcgcgcg cccaccagtt cgtcgagccg gctcagctca
     8101 cgatcggaca ttccgaaggg gaggcacaac tcgacgaggt tgcactgcga acaggccgat
     8161 ttgagcccat ccacggtaat tggcacgcta gccctcatct gcacgataat cctgctttcc
     8221 tgacattaga gacgcctgcg ctggagtggc gggattgcta atcacacccg ttgacccaag
     8281 tcaaccaaca ttacggaggc ttctgccatt gttcggcaga cagcccgcca gcaagatcat
     8341 gaaaagccag accgacctca tcttcgaccc gcaactgatc cgcaaattcg acatcaacgg
     8401 gccgcgctac acgtcctatc ccacggcgga ccgcttcgtc gaagccttca atgcagaagc
     8461 gctgcgggcc tggctggcca agcgggcggt gggcggcgta agcaaaccgc tctcattata
     8521 cttccacatc cccttctgca acacgatctg ctactactgc gcctgcaaca agatcattac
     8581 caaggatcat gggcgctcgg ccaagtacct gaaatatctc gcgaaagaaa tcgagatgca
     8641 ggccgcttgc ctggagggga gccgccaggt cacccagctc cacctcggcg ggggcacgcc
     8701 gacctttttg tcgcacgacg agatgcgcga gctcatggcg gcggtgcgcg agcacttcac
     8761 gctggtgccc aacggcgaat actcgatcga ggtcgacccg cgcaaggtcg atttcgacac
     8821 cgtgaagctg ctcgccgagc tcggcttcaa ccgcatgagc gtgggcgtgc aggatttcgc
     8881 cgaggacgtg cagcaggcgg tcaaccgcat ccagagcttc gacgagacca agctggtgat
     8941 cgacgcggcg cgcgccaacg gcttcaaatc ggtgagcatg gacctgatct acggcctgcc
     9001 gcggcagaac ccgaccagct tcgaccgcac gctcgaccag gtcctcgaga tctcgcccga
     9061 ccgcatctcg ctgtacagct acgcccacct gccgggcctg ttcaagccgc agcgccgcat
     9121 caacagcggc gacatgccca cggccgacac caagctcgcg ctactgcagc tcggcatccg
     9181 ccggctcacc gaggcgggct acgtgtatat cggcatggac cacttcgcca agcccgacga
     9241 cgagctcacc atcgcccagc gccagggccg cctgcaccgc aacttccagg gctattccac
     9301 ccacgccgaa tgcgacctgc tcgccttcgg ggtgtcggcg atcggcaaga tcggtccggt
     9361 gtacgcgcag aacgtgaaga cgctcgacga gtactacgac gcgctcgacc gcgacgagct
     9421 gccggtgctg cgcggcatcg agctcaccgc cgacgacctg ctgcggcgcg cgatcatcca
     9481 ggcgctgatg tgccacttcg agctctcgat gcagtcgatc gagatcgcct acctggtcaa
     9541 cttccgcgag tacttcgccg aggaactcgc cgatctgcag gaaatggaga aggccgggct
     9601 ggtcaagatc gacggcgact ggatcagcgt gcagcccggc ggccgcctgc tggtgcgcgg
     9661 catcgcgatg gtgttcgacc gctacctgcg cgccgaccgc gagcgccagc gctactcgcg
     9721 ggtgatctga acgccgcacg gattgcgcac cgcagcgtag aatgagggcc tcgcatttcc
     9781 cgcggggccc ttttcttctt catgcccgaa accggttatc tcgccgtctt cctgatcggc
     9841 ctgcttggcg gcacccactg cgtgtcgatg tgcggcggca tcgtcggcgc gctcagcatg
     9901 caggtgcagg tgcccggcag ccggccgcag tggccgctgc acctggccta caacctcggg
     9961 cgcatcggca cctacacctt gctcggtgcc ggtgtcggcc tgctcggctc ggtcggcctg
    10021 ctctacgagg gcatgctgcc ggtgcagatg acgctgtacg tgctcgccaa cctgatgctg
    10081 gtggcgctcg ggctctacct caccggcttc acccgcctgc tcgcgcccat cgagcgcgtc
    10141 ggtcacctgc tgtggcggcg catccagccc gccacccggc gcttcctgcc ggcgcgctcg
    10201 gtcgggcagg cgctgccgct cggcatgctg tggggcttca tcccctgcgg gctcacctat
    10261 tccatcctcg ccactgcgct cgtcaccgga tcgggtgcac gcggcgccgg cctgatgctg
    10321 gccttcgggc tgggcacgct gcccaacctg ctgctggcag gcatgctctt caaacgcttc
    10381 cgcgacatcg cccgccaccc cagggtgcgc ctggcctccg ggctcctcgt gctcggcttc
    10441 ggcctgttcg ggctgtacca cgccccctcg ctcggtggcg acctgtggcg cggcgtggtg
    10501 tgcgtgacct gagggtgcga cggcacgccc ctttcaaccc atcgggagta ctggataaat
    10561 gaacgaaacg acgatcaagg tcgaaggcat gagctgcggc ggctgcgtga agaacgtcac
    10621 cggcgtgctg aaggcgctgc ccggcgtgga ggacgtgcgg gtgtcgctgg aaggcgcatc
    10681 cgccaccgtg cgccacgatc cggccaaggt gtcgatcgac gcgttgcgcg ctgcggtcga
    10741 gggcgcgggc ttcgattccc cggcctgatc ggcagccttc gccgggggca tcgtcgcagt
    10801 gcaagacatt gtggtttccc cctataatcc gcgcacaatt tttccacggg agggatgtat
    10861 gggattcgaa caggtcagcg ccggcaagga cgtgccgaac gacatcaacg tcatcatcga
    10921 gatctccgcc cagggcgatc cgatcaagtt cgaggtggac aaggacagcg gcgccgtctt
    10981 cgtcgaccgc ttcatgggca cctcgatgcg ctacccgctg aactacggct acgtcccgca
    11041 caccatcgcc ggcgatggcg acccggtcga cgtgctggtc gtgaccccgt tcccgctgca
    11101 gccgggcgtg gtcatccgct gccgtccggt cggcgtgctg aagatggaag acgacggcgg
    11161 tgtcgacgcc aaggtcgttg ccgtgccggt gtcgaaactg accccgctgt acgacaaggt
    11221 gcagaccacc gacgacctgc ccgagctgct gatgaagcag acggtgcatt tcttcgagca
    11281 ttacaaggac ctcgagcccg gcaagtgggt caaggtcctg ggctggggca cggtcgagga
    11341 tgccaaggcc gagatcgtga acggcctggc ggctgccaag aagtaatccc gggaacgcac
    11401 gtttccggat gacccggggc ccacgcatgc gtgggccccg gccgtttacg gcgcggctga
    11461 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccatgctt gcgctgctga
    11581 tgaagatcaa caagaacctg atcctcgcca tcccggtggc gatggttctc ggcttcggct
    11641 tcgggctgct cgcgccggtc ggctggctga agtcgctgat cgtgccgctg accttcctga
    11701 tggtctatcc gatgatggtc aacctgaagc tctccaaggt cctcgaaggc ggcgacctca
    11761 aggcgcaggc gctcgcgcag gcgatcaact tcgccctcat ccccttcgtc gcctatgcgc
    11821 tcggcaaggc cttcttccct gaccagcccg cctacgcgct cggtttgctg ctcgcggcgc
    11881 tgctgcccac cagcggcatg acgatctcgt ggaccggctt cgccaagggc aacctcggtg
    11941 cggcggtcaa gatgacggtg ctcgggctca tcctcggctc gctcgccacg ccgctctacg
    12001 tgcgctggct gatgggggcc gaggtgccgg tcgacatggg cgcggtgtcc atgcagatcg
    12061 tggtcatcgt cttcctgccc atgctggccg gccactacac gcagaaatgg ctggtcgcgc
    12121 gccacggcca ggccgaatac cagaaggaat gggggccacg cttcccgccg ttctccaccc
    12181 tcggcgtgct cggcatcgtc ttcgtcgcgc tcgccctcaa ggcgaaggac ctcgtcgccc
    12241 agccgggcga gctgatcgtg gtgctggtgc cgctgctgtg cctgtacgcg atcaactacg
    12301 tggtgagcac ggtggtcgcg cgcagcctgc tgccgcgcgg cgacgccatc gcgctcgtgt
    12361 acggcacggt gatgcgcaac ctgtcgatcg cgctcgcgct ggcgatgaac gtcttcggcg
    12421 cctcgagctc gggtgcggcg ctggtgatcg cgctggccta catcctgcag gtgcagtcgg
    12481 cggcctggta cgtgaagctc accgatcgct tcttcggccc cgccgcaccc gcgaaggcgg
    12541 ggggctgagc gctggtgcgg acggagcgcc cgggcttccg gccgcacttc gatccctcgc
    12601 ccggggaggt aggtgccacc ggcactcccg cgcgctgaca acgcttacgc caaacgaccg
    12661 gcccggcccg cgattcagcc cggaatccgc gccacgtgca gaaggttggt gctgccgcgc
    12721 tggccgaagg gcaggccggc gatcaccacc accgcgtcgc cggcgctgcc gaagcccaga
    12781 ttctgcaccg cgcgcgcggc atgctcgacc atctcggcaa cgtcatgaac ctcctcgaag
    12841 ggcaccgcat gcacgcccca caccagcgcc aggcggcgcg cggtggtggg ctgcggcgtc
    12901 agcgccagga tgggcgcgac cggacgttcg cggctggcgc gcagggcgct gaagcccgag
    12961 ctcgtgtagg ccaccgtggc cgccggttcg agcaggccgg cgacgcggcg cagcgcgcag
    13021 cagatcgcgt ccggggtgtt cgccgcggcc gccgtgtggc tggcttccag tccggcgcgc
    13081 caggcggggt cgcgctccac ctcgcagacg atgccgtgca tgatggccac cgcctccacc
    13141 gggtactggc ccgaggccga ctcggccgac agcatcaccg cgtcggcgcc gtcgtagatc
    13201 gcggtggcca cgtccgaggc ctcggcgcgg gtgggcacag gcgaggagat catcgactcc
    13261 agcatctgcg tcgccaccac caccggacgg ccggctgcgc gtgcggcccg cacgatgcgg
    13321 cgctgcagca ccggcacctg ctgcggtggc agctccacgc cgaggtcgcc gcgcgcgacc
    13381 atgacgccgt cggcgagcgc gatgatcgcg tcgagcgcgt cgatcgccgc cggcttctcg
    13441 agcttggcca tgatccaggc gcggtcgccg atgagttcgc gcgcctcgcg cagatcctcg
    13501 gggcgctgca cgaaggacag cgccacccag tccacgccca gcgccaggcc gaagtcgagg
    13561 tcggcgcggt ccttcgtggt gagcggcgag atcggcagca ccacaccggg cacgttcacg
    13621 cccttgcggt cggacagcgt cccgcccacc agcacggtgg tgtcggcgaa gtcggcgccg
    13681 caggcgtcca cccgcaggcg cagcttgccg tcgtcgagca gcagctcggt gccggcctcc
    13741 agcgcggcga agatctcggg gtggggcagg ttggcgcgct tggcgtcgcc ggccgtgggg
    13801 tcgaggtcga ggcggaagcg tgcgccgggc accagggcga ccttgccctc ggcgaagcgg
    13861 ccgacgcgca gcttgggccc ctgcaggtcc atcagcacgc cgatcgggcg gccgatctcg
    13921 gcctcgaccg cgcggatcag gcggtagcgt tcggcgtggt cctcgtggct gccgtggctg
    13981 aagttgagcc ggaacacgtc cgcgccggcc tcggccaggg cgcggatgcg ttcgcgggtg
    14041 gagctcgccg gcccgagggt ggcgaggatg cgggcgctgc gttcgcgttt catgtcgggt
    14101 cctcggcggt tcaggcggcg gatcgggggt gggggagggc gtgtgcgagc agggcgtcga
    14161 ccccggcgcg ggcgacgcgg gcgtcctcgc cggccttcac gccggagacg cccaccgcac
    14221 cgagcacgcg gccatcgacc accaccggct cgccgccttc gagcggcacc accggcatcg
    14281 ccagcgcggc gaagcggccg ttgttgacca tgtcctcgaa ggccttgctc ggcttgccgc
    14341 tgagcacgca ggtgcgcgcc ttctcggggg cgacgatggc gctcatcagg ggtgcgccgt
    14401 ccatgcgctg cagccacagg gcgtggccgc ccgcatcgca gatcgcgatg ctgaccgccc
    14461 agccgttgg
//
LOCUS       DAOQNN010000034        14466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1703_length_14466_cov_6.640067, whole genome shotgun sequence.
ACCESSION   DAOQNN010000034 DAOQNN010000000
VERSION     DAOQNN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 14466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14466
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1703_length_14466_cov_6.640067"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKV97_03800"
     CDS             <1..372
                     /locus_tag="PKV97_03800"
                     /inference="COORDINATES: protein motif:HMM:NF022181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 family protein"
                     /protein_id="HNB05030.1"
                     /translation="AADAAAVAQQREAALAATIAESQRRTAAVEESAHVADLAASAAR
                     SDAARSAALARRLREQLAAGRAASAPAGDHPASAGAGQADRLADVLGQCVERYRAVAA
                     DADAAVVAGRACERAYDALTR"
     gene            463..696
                     /locus_tag="PKV97_03805"
     CDS             463..696
                     /locus_tag="PKV97_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05031.1"
                     /translation="MRLYLRLHAHRVRTGARGYLSFRRSDLAKMSPHIVYTELTRSGR
                     VRMVSYVPVEPVRRILPPVIFRGRVRWGDGHDG"
     gene            complement(1021..1281)
                     /locus_tag="PKV97_03810"
     CDS             complement(1021..1281)
                     /locus_tag="PKV97_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05032.1"
                     /translation="MQSLRSPETARYHALRIGRWLEGRRASEARAFAAVACSRPGGPV
                     SKPKPTSARKARPYQRSRTASPVLGASLTRLSLATSPNERDT"
     gene            complement(1377..1658)
                     /locus_tag="PKV97_03815"
     CDS             complement(1377..1658)
                     /locus_tag="PKV97_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB05033.1"
                     /translation="MTTHSMLTAAELAAHLRTSERTVARLVADGCPSLKVRGRRIFDL
                     ATVLTWLAEAEACRSEETLKATGTPRLASAVVAFTDACRRVQVRATPSN"
     gene            complement(1655..3025)
                     /locus_tag="PKV97_03820"
     CDS             complement(1655..3025)
                     /locus_tag="PKV97_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB05034.1"
                     /translation="MSRPFVPRPYQRAMVEHAHAHARGALFAGMGLGKTVATLTALAD
                     LELTEPGPALVVAPLRVAQSTWPDEAAKWGHLAGFEVVPVVGSLSQRRAALRRRADVY
                     TTNYEQTPWLVEELGGRWPFRKIVADESTRLKGFRLRQGTRRAKALAKVAHEHCTHWV
                     NLTGTPAPNGLQDLWGQTWFLDRGQRLGRTFDSFAQRWFRPAYNGFGVEPLAHARGEI
                     EERLRDVCLTIDARDHFDLAEPIVSTIRVELPAKARALYRDMEREMFAQIGEHEVEAF
                     SAAARTIKCLQLASGAAYVDDSATEWVEVHDAKLQALDSIVAEAAGASVLVAYHFRSD
                     LARLQRAFPRGRHLDQDTQTIRDWNAGRIPVLFAHPASAGHGLNLQDGGNILVFFSHW
                     WDLEQYQQIIERIGPTRQAQAGHERPVFVHHIVAADTVDEIVMARRESKREVQDLLLE
                     AMKARG"
     gene            complement(3022..3342)
                     /locus_tag="PKV97_03825"
     CDS             complement(3022..3342)
                     /locus_tag="PKV97_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05035.1"
                     /translation="MKALRERDIERHLVLRVKAAGGEVRKVQWIGRRGAPDRLVMLPA
                     RRLSNTLDCAWCNPRGLAIWVELKAPGVQPEPHQRREHERMRAMGQRVEVIDSLQAVE
                     ALLP"
     gene            complement(3339..3524)
                     /locus_tag="PKV97_03830"
     CDS             complement(3339..3524)
                     /locus_tag="PKV97_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05036.1"
                     /translation="MKRFCCDGLCAQGRDCPVVDERRPPMSRAEAWVAVAIGALSAAV
                     SIAIVVALAGVVLGWLS"
     gene            complement(3528..5633)
                     /locus_tag="PKV97_03835"
     CDS             complement(3528..5633)
                     /locus_tag="PKV97_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05037.1"
                     /translation="MTTLFLDLETYSETPIRNGTYAYAERAEVMLWAWALDDGPVSVW
                     DLTTSPAPPTPLLLALEDGRVEIWAHNAQFDRTVLRLAAPFQGARIAAQDISRWRDTR
                     AKALCHGLPGELAALCSILDVPVDQAKDKAGRQHMLLFCKPRADGTRATARTHPVEWQ
                     RFVEYAGQDIVAMRAVDAKLPQWNYRGAELALWHLDQTINDRGVAVDLELTEAAVAAV
                     AAAQKVLAKRTQALTAGDVTSATQRDAMLAHILAEYGVGLPDLRADTVERRAADPDLP
                     PELRELLALRLQASTTSAAKYATLLRGASIDGRLRGTLQYSGAQRTRRWSGRLFQPQN
                     MPRESMPRDDIAQGIEALKAGCADLVTDNVMALASNAVRGCLVAPAGRKLVVSDLANI
                     EGRVAAWLGGEQWKLDAFARYDAGEGPDLYVVAYARAFAVPPESVGKAERQIGKVMEL
                     MLQYEGGVGAFVTGAATYGVDLDELAERAWGSLPAQVREEATEFLAWCRREKRSTYGL
                     SDGAFIVCDALKRMWRAQHEGIVGLWGDLRSAAINATQWPGQTYRAGPRRVDADGKTR
                     AAFAVRRDGNWLRILLPSGNYLCYPAPEVRDGALAYMGVDQYSRKWSRLATYGGKLFE
                     NACQSLARDVLAGAMPHIEAAGYEIVLTVHDEVICEAPDTDDYSVAGLSALLAANPPW
                     ASGLPLAADGFEAHRYRKG"
     gene            complement(5630..6094)
                     /locus_tag="PKV97_03840"
     CDS             complement(5630..6094)
                     /locus_tag="PKV97_03840"
                     /inference="COORDINATES: protein motif:HMM:NF019106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 motif-containing HNH endonuclease"
                     /protein_id="HNB05038.1"
                     /translation="MAKDHDRELKHQQQSEHGEMQVEIWKDVPGYEGRYQVSDEGRVR
                     SLAHHVRLVAHGKETVRLSPGRVLKPGPQQSGHLSVAIGKGNSRQVHQLVLEAFVGPC
                     PEGCEVLHLNHNPADNRLANLRYGTRSENLRMDYAAGRRTVAQNFIGARWRA"
     gene            complement(6182..6733)
                     /locus_tag="PKV97_03845"
     CDS             complement(6182..6733)
                     /locus_tag="PKV97_03845"
                     /inference="COORDINATES: protein motif:HMM:NF022444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HNB05039.1"
                     /translation="MRLALKNVRLAFPSLFEAKTVNGEGEPAFSAVFLIEPADPQTKA
                     VEQAIAAVAKDKFGAKTDAVLKAMKAADKTCLHDGNTKAEYAGFADMLFVSARGKVRP
                     LVLDSDKSPLTAADGRPYAGCYVNASIDLWAQDNSFGKRVNAQLRGVQFLRDGDAFAG
                     GGGAATEDEFDDVAEGATADDLT"
     gene            complement(6745..8001)
                     /locus_tag="PKV97_03850"
     CDS             complement(6745..8001)
                     /locus_tag="PKV97_03850"
                     /inference="COORDINATES: protein motif:HMM:NF022379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HNB05040.1"
                     /translation="MSRALLPPSSAHRWLRCPGSVAMEAGLPDTSSAFADEGTAAHTL
                     AAMCLRDGVGASEYLGEVIIAGERRFQVDSDMAEHVQRYLELVRDYALLGDLLIEQRV
                     EFGRHIGEASAFGTSDAIVVAEDGDELIIIDLKYGRGVKVDAEGNEQLMLYALGALDL
                     IEPLGYEVKRVRLVIHQPRLAHASEWECGVAALLAFAERAKAAASHAMACIQLGVDEA
                     EDLMPGEKQCRWCKAKATCGALRDAVAEPVFGTAPATPEEFDEAPVPGAKHIEPTTDE
                     WLSAAMGKVELIEGWCKAVRAEAERRLLAGQPVQGWKLVEGRRGSRKWADEKAAEEAL
                     KAMRIKHDQMYDYSVISPTSAEKLAKAEVIGPRQWPKLQALITQSDGKPSVAPEADKR
                     PALVVTPTADEFEAVNTTAATADDLV"
     gene            complement(7998..8423)
                     /locus_tag="PKV97_03855"
     CDS             complement(7998..8423)
                     /locus_tag="PKV97_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05041.1"
                     /translation="MYPMTITIANAAQFDAVRRALDADAVRQSMHDEATAEAPIGAFD
                     APAPSPAAKGKERKSAPKAEPTKAKAEQPTPAAEPHAEQPVATYEQVKTAILEVSRAK
                     GRDAAVALLAQFGAARGPELQDKPETFGPFVAAAKALLS"
     gene            complement(8428..8817)
                     /locus_tag="PKV97_03860"
     CDS             complement(8428..8817)
                     /locus_tag="PKV97_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05042.1"
                     /translation="MNLGRLTDEELLRHAHVTLDGLTSTALEVELLLRLERHVAAAAE
                     QEAYRALLDKNDIDSVADLKAQLDKAEALDEHIDGLALLGVLRDFDIDDPAALTTILK
                     RDAALGNVLDDLAQPLASLQALITTEQ"
     gene            complement(8814..8996)
                     /locus_tag="PKV97_03865"
     CDS             complement(8814..8996)
                     /locus_tag="PKV97_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05043.1"
                     /translation="MSDLPVPYLPYFTELALARDWPPGWLPHGEVEGIEAAVEKKEAK
                     LQRARESERQRRRETP"
     gene            complement(8993..9178)
                     /locus_tag="PKV97_03870"
     CDS             complement(8993..9178)
                     /locus_tag="PKV97_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05044.1"
                     /translation="MNALPEHRRMLAGRTWSRSYSRSHYAPLSGPWRRHERTADVLLA
                     VALGVVVALSLLHWWAA"
     gene            complement(9175..9573)
                     /locus_tag="PKV97_03875"
     CDS             complement(9175..9573)
                     /locus_tag="PKV97_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05045.1"
                     /translation="MSATLENAMVSGLGGYYARQERRDALAESAYVSLRDELIDALTL
                     DPAREVSTPGFGKRRSTATAIDVIGEAIDSKGGEQVLHELLRIVAMCAARAADHELHL
                     RASAWIAARAAAHAAYHVDDLVAEIEEGDA"
     gene            complement(9570..10322)
                     /locus_tag="PKV97_03880"
     CDS             complement(9570..10322)
                     /locus_tag="PKV97_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05046.1"
                     /translation="MAAAKKAEVAVDANPAQTQEPTETIVAYKGFDRNMQCRGYQFAI
                     GQTYEHDGKVEACASGFHACEHPLDVLEYYAPSESRYAVVEQRGDLSRHGEDTKVASR
                     LITIKSEIDIAGLVRAAIEWTTKRCLPIDPASPASAAGYRGAASATGDRGAASATGYQ
                     GAASATGYRGAASATGDQGAASATGNRGVAVACGAFGRAMAAASGAIVLSARNADGSI
                     RYIRASKVGDNGVEPDVWYSLNEAGEFVAVPQ"
     gene            complement(10447..10893)
                     /locus_tag="PKV97_03885"
     CDS             complement(10447..10893)
                     /locus_tag="PKV97_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05047.1"
                     /translation="MAADFSVYSDSAQVNTALVSTKVRLVDDPTDAEQEERRVRLRAW
                     VADHGGHAKVTEQFHLTASQASFLSQVTNGYSFASRAARNMERRLRMPVRWLDGTVVT
                     ARPGAAVASGASTTITAPTAPTLEEALRVALAALRDLPPTSTPART"
     gene            10892..11146
                     /locus_tag="PKV97_03890"
     CDS             10892..11146
                     /locus_tag="PKV97_03890"
                     /inference="COORDINATES: protein motif:HMM:NF027273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HNB05048.1"
                     /translation="MDFPSWLDADRGRLTSVAEHFGLTQSAVSQWRQNGVPPGRMKAV
                     RELTGGAVTLDDMLPDADRPHPQGRPALDVDAPKHAATAV"
     gene            11168..11416
                     /locus_tag="PKV97_03895"
     CDS             11168..11416
                     /locus_tag="PKV97_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05049.1"
                     /translation="MTSTGDPFVELRGEILREHVDVIDAVVQATPGASRMSVLRRIVA
                     EWADRKVHEASLIHRVAGNNGTAPQACRHRNGSRAETP"
     gene            11413..13380
                     /locus_tag="PKV97_03900"
     CDS             11413..13380
                     /locus_tag="PKV97_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB05050.1"
                     /translation="MSVVAIKPHLTAMWAPDELRRLVGWLVWRYELRDGEAKPRKVPY
                     YAGGGKRHGVNGCPEDRAQLVTFDAACAAAAKRGFDGVGLALMPEFGVCVLDFDRCLA
                     AGGLHPEVLALVQVTYAEFSPSGQGVHAFFTGDLGNHKDHGEPFGFEVFSSRGFVTFT
                     GNSLPHVEGLDLIGPPVAPVTDAVRALCARRFGPRAVPDDDDPLMSYSPPLGLTEGQL
                     REALDVLDAGMRHDPWLHVGMALHHETGGEGFDLWDQWSSTAPSYPGRENLLKRWESF
                     GQRAGKVVTARSLVKLANEHGARINVATSLAEFDAPAPHKPDRAQRFPLVQAAQFADG
                     AAPRWIIKDVLPQAELVVLFGESGAGKSFAALDMAGAIARGVPWRGHKVRKGRVVYVA
                     AEGSGGMRKRLKAYARHHNLDLADIELHVVPAAPNLLLKEDALALARVIATLGPVDVI
                     VIDTLAQTTPGGNENAAEDMGRALAHCRGIHRATGAVVVLVHHAGKDTSKGARGWSGL
                     RAAADAELEVVRLGAARVLQVTKQKDGDDHGRWAFDLVEVALGMDEDGDVVSSCVVVE
                     AAMPATGQVGKPLGKIEQVVNEVIQEFAQAQTAGIEVAAVLAEAVKRLPEPEDGKRDT
                     RKQHAKRALAALCEGDEAAYWMRDDGTLEVM"
     gene            13901..14155
                     /locus_tag="PKV97_03905"
     CDS             13901..14155
                     /locus_tag="PKV97_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05051.1"
                     /translation="MTGRVDWECVLADLAWVLGEPMPGSAERTPLGTRALAERLRVSR
                     GALRNWCAGTEPRHSDALRLLQRWSELTGKPASMAPVRRA"
     gene            14190..>14466
                     /locus_tag="PKV97_03910"
     CDS             14190..>14466
                     /locus_tag="PKV97_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05052.1"
                     /translation="MCNPLLVVAAASVASAAYSADQQRKAVHSQQDALAASQAEDARK
                     AAEAETGAQVAANANLADAKRRRRTSALGAGDPNAGTSDTLGGTSSVL"
BASE COUNT         2019 a         5009 c         5168 g         2270 t
ORIGIN      
        1 gcggccgatg ccgctgccgt ggcgcagcag cgcgaggcgg cgctcgccgc caccatcgcc
       61 gagtcgcaac gccgcaccgc ggccgtagag gaatccgccc atgtcgccga cctcgccgcg
      121 agcgctgctc gctctgacgc tgctcgctcc gctgctctgg ctcgccggct gcgcgagcag
      181 ctcgccgccg gccgtgctgc ctctgcacca gcaggcgatc atcccgcctc tgccggcgca
      241 ggccaggcag accggctcgc cgacgtactc ggccagtgcg tcgagcgcta tcgagcagtg
      301 gctgctgatg ctgacgccgc cgtcgtcgcc ggccgggcct gcgagcgcgc ctacgacgcg
      361 ctgacgcgct gacgtgcgat ggtggcgacg ggcgatgatg gcgctccgtg gtggcgccgg
      421 acgtggctgc tcgccaggcg cacgaactgc ctcctttggg gcgtgcggct gtatctgagg
      481 ctgcatgcgc accgggtgcg cactggcgcc aggggttatc tgagctttcg gcgcagcgac
      541 ctcgccaaga tgtcgccgca catcgtctat accgagctga cgcgcagcgg ccgggtgcgc
      601 atggtctcct acgtgccggt cgagccggtg cgccgcatcc tgccgccggt gatcttccgc
      661 ggccgcgtgc gctggggcga tggccacgac gggtgaatgc ccgctgtgcc gcgggccgca
      721 ccggctgagc cagtgcccgc gctggcggtt ctgtcggtgg gggactttct tcctactgcc
      781 gctcgcgcta cgccggtaga gcgcagcacg cgcatcctgt gccgcccatc gcgttgcgct
      841 tccacgccca cctgcacgcg gcgcgcgggt aggcgatgcg gttcttgatc gtggccgggg
      901 ccagcgcgcc ggactggtcg gccgcgtact cggcgcagac gtcgggcagc tcctcgatgc
      961 cgcgtccggc ccaccagtcg cgcgtcgcgt cgagttcgcg cgcgacgttg ccgccgctct
     1021 tcaggtgtcg cgctcgttcg gcgacgtagc gagcgacagc ctcgtcaatg aggcgccgag
     1081 gacgggcgat gccgtgcgcg agcgctgata gggccgcgct ttccttgcgc tcgtaggctt
     1141 cggcttgctc acgggtccac ccggccggga gcaggcgacg gcggcgaagg cgcgcgcctc
     1201 gctggcccgg cgaccctcga gccagcggcc gatgcgcagc gcgtggtagc gggccgtctc
     1261 gggactgcgc agggactgca cgtagttctc ggcgtagtcg gcgacgagcg cggtgagcag
     1321 aggatcgccg accgtcgctg gcgcggcggt gtgcagcgcg tggacgagct cggcctctaa
     1381 ttgcttggcg tcgcgcgcac ttgcacccgc cggcaggcgt cggtgaaggc gacgacggcg
     1441 gacgcaagcc tcggcgtgcc agtagccttg agcgtctctt cggatcggca tgcttcggcc
     1501 tccgcgagcc aggtgaggac tgtagcgagg tcaaagatgc gtcggccgcg caccttcaga
     1561 ctcgggcagc cgtcggcgac caggcgcgca acggtgcgct cgctcgtgcg caggtgtgcg
     1621 gcgagttcgg cagcggtgag catcgagtgc gtcgtcatcc cctggccttc atcgcttcga
     1681 gcagcaggtc ctgcacctcg cgcttgctct cgcggcgcgc catcacgatc tcgtcgacgg
     1741 tgtcggccgc gacgatgtgg tgcacgaaca ccggccgctc gtggccggcc tgcgcctggc
     1801 gcgtcgggcc gatgcgctcg atgatctgct ggtactgctc gaggtcccac cagtgcgaga
     1861 agaagacgag gatgttcccg ccgtcctgca ggttcaaccc gtgaccggcg ctggccgggt
     1921 gcgcgaagag caccgggatg cggccggcgt tccagtcgcg gatcgtctgc gtgtcctggt
     1981 ccaggtggcg gcctcgcgga aacgcgcgct gcagccgcgc caggtcggac cggaagtggt
     2041 acgccaccag caccgacgcg ccggcggcct cggccacgat gctgtccagg gcctgcagct
     2101 tggcgtcgtg cacctcgacc cactcggtgg cgctgtcgtc gacgtaggcg gcgccgctgg
     2161 cgagctgcag gcacttgatc gtgcgcgccg cggcgctgaa ggcctcgacc tcgtgctcgc
     2221 cgatctgcgc gaacatctcg cgctccatgt cgcggtacag cgctcgggcc ttggccggta
     2281 gctcgacgcg gatggtgctg acgatgggct cggctaggtc gaagtggtcg cgggcgtcga
     2341 tcgtcaggca cacgtcgcgc agccgctcct cgatctcacc gcgggcgtgc gccagcggct
     2401 cgacgccgaa gccgttgtac gcgggccgga accagcgctg cgcgaagctg tcgaaggtgc
     2461 gcccgaggcg ctggccgcgg tcgaggaacc aggtctgacc ccacaagtcc tgcaggccgt
     2521 tcggcgccgg cgtgccggtc aggttgaccc agtgcgtgca gtgctcgtgc gccaccttcg
     2581 ccagcgcctt cgcgcgccgc gtgccctggc gcagccggaa gcccttgagg cgcgtcgact
     2641 cgtcggccac gatcttgcgg aagggccagc gcccgccgag ctcttcgacg agccaggggg
     2701 tctgctcgta gttcgtcgtg tacacgtcgg cgcgccggcg cagcgccgcg cggcgctgcg
     2761 aaaggctgcc gacgacgggc acgacttcga agccggccag gtgaccccac ttggccgcct
     2821 cgtcgggcca cgtggactgc gccacgcgca gcggcgcgac gacgagcgcc gggccgggct
     2881 cggtcagctc gaggtcggcc agcgccgtga gcgtggcgac ggtcttgccc aggcccatgc
     2941 ccgcgaatag cgcgccgcgc gcgtgcgcgt gggcgtgctc gaccatcgcg cgctggtatg
     3001 ggcgagggac gaagggcctg ctcacggcag cagcgcctcc acagcctgca aggagtcgat
     3061 gacctccacg cgctggccca tcgcgcgcat gcgctcgtgc tcgcgccgct ggtgcggctc
     3121 gggctggacg cctggcgcct tgagctcgac ccagatcgcg aggcccctcg ggttgcacca
     3181 ggcgcagtcg agcgtgttcg acaggcgccg cgccggcagc atcaccagcc gatccggcgc
     3241 gccacggcgg cctatccact gcaccttgcg cacctcgccg cccgctgcct tgacgcgcag
     3301 cacgaggtgg cgctcgatgt cgcgttcgcg cagggccttc acgacagcca ccccaacacg
     3361 acgccggcca gcgccacgac gatggcgatc gacaccgccg cgctcaaggc tccgatggcg
     3421 acggcgaccc aggcctcggc gcggctcatc ggtgggcgcc gctcgtcgac cacagggcag
     3481 tcgcgaccct gagcgcacag gccatcacag cagaagcgct tcatccgtca ccctttgcgg
     3541 tagcggtgcg cctcgaagcc gtcggccgcg agcggcagac cggatgccca aggcgggttg
     3601 gcggcgagca gcgccgacag gcctgcgacg ctgtagtcgt cggtgtccgg cgcttcgcag
     3661 atcacctcgt cgtgcacggt cagcacgatc tcgtagccgg cggcctcgat gtgcggcatc
     3721 gcgccggcga gcacgtcgcg ggccaatgac tggcacgcgt tctcgaagag cttgccgccg
     3781 taggtggcca ggcgcgacca cttgcggctg tactggtcga cgcccatgta ggcgagcgcg
     3841 ccgtcgcgca cctcgggcgc cgggtagcac aggtagttgc ccgagggcag caggatgcgc
     3901 aaccagttgc cgtcgcggcg cacggcgaac gcggcgcgcg tcttgccatc ggcgtcgacg
     3961 cggcgagggc cggcgcggta ggtctgaccc ggccactgcg tggcgttgat ggcggcagag
     4021 cgcagatcgc cccagaggcc gacgatgccc tcgtgctgcg cgcgccacat gcgcttgagc
     4081 gcgtcgcaga cgatgaacgc cccatcgctc agtccgtagg tcgagcgctt ctcacggcgg
     4141 caccacgcca ggaactcggt cgcctcctcg cgcacctgcg cggggagcga accccaagcg
     4201 cgctcggcca gctcatcgag gtcgacgccg taggtggcag cgcccgtgac gaacgcgccg
     4261 acgccgccct cgtactgcag catgagttcc atcaccttgc cgatctggcg ctcggccttg
     4321 ccgacgctct cggggggcac cgcgaaggcc cgcgcgtagg cgacgacgta gaggtctggg
     4381 ccctcgccgg cgtcgtaccg ggcgaaggcg tcgagcttcc actgctcgcc gccgagccag
     4441 gccgcgacgc ggccctcgat gttggccagg tccgagacga cgagcttgcg ccccgcaggc
     4501 gccaccaggc agccgcgcac ggcgttgctg gccagggcca tcacgttgtc ggtgacgagg
     4561 tcggcgcagc cggccttgag cgcctcgatg ccttgcgcga tatcgtcgcg cggcatgctt
     4621 tccctaggca tgttctgcgg ctggaacagg cgcccagacc agcgcctggt gcgctgcgcg
     4681 ccgctgtact gcaacgtgcc gcgcaggcgg ccgtcgatgc tcgcgccgcg cagcagcgtg
     4741 gcgtatttcg cggcgctggt ggtgctggcc tgcagccgga gggcgagcag ctcgcgcagc
     4801 tcgggcggca ggtccgggtc ggcggcgcgc cgctcgacgg tgtcggcccg caggtcgggc
     4861 agaccgactc cgtactcggc cagaatgtgc gcgagcatcg cgtcgcgctg ggtggcgctg
     4921 gtcacgtcgc cggcggtcag cgcctgcgtg cgcttggcga ggaccttctg cgccgcggcc
     4981 acagccgcga ccgctgcctc ggtcagctcg agatcgacag ccacgccacg gtcgttgatg
     5041 gtctggtcaa gatgccacag cgccagctcg gcgccgcggt agttccactg cggcagcttc
     5101 gcgtcgacgg cgcgcatcgc cacgatgtcc tggccggcgt actcgacgaa gcgctgccac
     5161 tcgacggggt gcgtgcgcgc cgtggcgcgc gtgccgtcgg cgcgtggctt gcagaacagc
     5221 agcatgtgct gccgcccggc cttgtccttg gcctggtcga cgggcacgtc gaggatgctg
     5281 caaagcgccg cgagctcgcc gggcaggccg tggcacagcg ccttggccct cgtgtcgcgc
     5341 caacggctga tgtcctgcgc ggcgatgcgg gcaccctgga acggcgcggc taggcgcagc
     5401 acggtgcggt cgaactgggc gttgtgggcc cagatctcga cgcggccatc ctccaacgcc
     5461 agcaggagcg gagtcggcgg cgcgggggag gttgtcaggt cccacaccga cacgggcccg
     5521 tcgtcgagcg cccacgccca gagcatgacc tccgcgcgct cggcgtaggc ataagtaccg
     5581 ttcctgatcg gggtttcaga gtacgtttcc agatcaagaa aaagcgtggt catgcccgcc
     5641 accttgcgcc gatgaagttc tgcgcaacgg tgcgacgtcc tgctgcgtag tccatgcgca
     5701 ggttttcgct gcgcgtgccg tagcgaaggt ttgccaagcg gttgtcggcg gggttgtggt
     5761 tgaggtgcag tacctcgcag ccttccgggc aagggccaac gaaagcctcg aggacaagct
     5821 gatgcacctg gcgggagttg cctttaccga tagcaacaga gaggtggccg gattgctgag
     5881 gcccaggttt caacacgcgg ccgggtgata ggcgcaccgt ttccttgccg tgggcgacta
     5941 ggcgcacgtg atgcgccagt gaacgcacgc gcccttcgtc gcttacctga taccggcctt
     6001 catatccggg aacatccttc caaatctcca cttgcatttc gccgtgttcg ctttgctgct
     6061 ggtgtttcag ctctcggtca tggtctttcg ccaagagcca agggcacttg gcgaaaaacc
     6121 cccgggcacg aggcccgggg aaagcgcagc tcgtgccacg catgagggac tcacaaacgg
     6181 atcaggtgag gtcgtcggcc gtcgcgcctt cggccacgtc gtcgaactcg tcttcggtcg
     6241 cagcgccgcc gccgccggcg aaggcgtcgc cgtcgcgcag gaactgcacg ccgcgcaact
     6301 gcgcgttgac gcgcttgccg aagctgttgt cctgcgccca gaggtcgatc gaggcgttga
     6361 cgtagcagcc cgcgtagggc cggccgtcgg cggcggtgag cgggctcttg tcggagtcga
     6421 gcaccagcgg gcgcaccttg ccgcgcgcgc tcacgaacag catgtcggcg aaaccggcgt
     6481 actcggcttt cgtgttgccg tcgtgcaggc aggtcttgtc ggcagccttc atcgccttga
     6541 gcacggcgtc ggtcttggcg ccgaacttgt ccttggcgac cgcggcgatc gcctgctcga
     6601 cggccttggt ctgcgggtcg gccggctcga tcaggaagac ggccgagaag gcgggctcgc
     6661 cctcgccgtt gacggtcttc gcttcgaaca gggaggggaa cgctaggcgg acgttcttca
     6721 gtgcaagtct cacggtgttt cctttcagac gaggtcgtcg gccgtggcgg ccgttgtgtt
     6781 caccgcttcg aactcgtcgg cggtgggcgt gacgacgagc gcggggcgct tgtcggcttc
     6841 gggcgcaacc gagggcttgc catcggactg cgtgatgagg gcctgcagct tgggccactg
     6901 gcgcgggccg atgacctcgg ccttggcgag cttctcggcg ctggtggggc tgatgaccga
     6961 gtagtcgtac atctggtcgt gcttgatgcg catggccttg agcgcctcct cggcggcctt
     7021 ctcgtcggcc cacttgcgcg agccgcggcg accctcgacg agcttccagc cctggaccgg
     7081 ctggccggcc agcagccggc gctcggcctc ggcgcgcact gccttgcacc agccctcgat
     7141 cagttcgacc ttgcccatcg ccgccgagag ccattcgtcg gtggtcggct cgatgtgctt
     7201 ggcgccgggc accggggcct cgtcgaactc ttcgggcgtc gccggcgcgg tgccgaagac
     7261 gggctcggcg accgcgtcgc gcaaagcgcc gcacgtcgcc ttggccttgc accagcggca
     7321 ctgcttctcg cccggcatca ggtcctcggc ctcgtccacg ccgagctgga tgcacgccat
     7381 cgcgtgcgag gctgcggcct tggcgcgctc agcgaaggcg agcagagcgg cgacgccgca
     7441 ctcccactcg ctggcgtgcg ccaggcgcgg ctggtggatg accaggcgca cgcgcttgac
     7501 ctcgtagccc aacggctcga tcaggtcgag cgcgccgagc gcgtagagca tcaactgctc
     7561 gttgccctcg gcgtcgacct tgacgccgcg gccgtacttg aggtcgatga tgatcagctc
     7621 gtcgccgtct tcggcgacca cgatcgcgtc gctcgtgccg aacgcgctgg cctcgccgat
     7681 gtggcggccg aactcgacgc gctgctcgat cagcagatcg ccgagcagcg catagtcgcg
     7741 cacgagctcg aggtaccgct gcacgtgctc ggccatgtcg gagtcgactt ggaagcgccg
     7801 ctcgccggcg atgatcacct cgccgaggta ctcgctggcg cccacgccgt cgcgcaggca
     7861 catcgccgcc agggtgtgcg ccgcggtgcc ctcgtcggcg aacgcgctgc tggtgtcggg
     7921 cagcccggcc tccatggcga cagagccggg gcagcgcagc cagcggtgtg ccgacgaggg
     7981 cggcaacaag gcgcggctca cgacagcagc gccttcgcgg cagcgacgaa ggggccgaac
     8041 gtctcgggct tgtcctgcag ctcggggccg cgggcggcgc cgaactgcgc gagcagcgcc
     8101 accgccgcgt cgcggccctt ggcgcggctc acttcgagga tggcggtctt cacctgctcg
     8161 taggtggcca cgggctgctc ggcatggggc tcggcggccg gggtcggctg ctcggccttg
     8221 gctttggtgg gctcggcctt gggcgcgctc ttgcgctcct tgcccttggc ggccggcgag
     8281 ggggcaggcg catcgaacgc cccgatgggc gcctcggcgg tggcctcgtc gtgcatcgac
     8341 tggcgcacgg cgtcggcgtc gagcgcgcgg cgcaccgcgt cgaactgcgc ggcgttggcg
     8401 atcgtgatgg tcatggggta catgcggtca ttgctccgtg gtgatgaggg cttgcaggct
     8461 ggccagcggc tgggccaggt cgtcgaggac gttcccgagc gccgcgtcgc gcttgaggat
     8521 cgtcgtcagg gcggcggggt cgtcgatgtc gaagtcgcgc agcacgccga gcagcgcgag
     8581 gccgtcgatg tgctcgtcga gggcttcggc cttatcgagc tgcgccttca gatcggcgac
     8641 ggagtcgatg tcgttcttgt ccagaagggc gcgatacgcc tcttgctcgg ccgccgcggc
     8701 gacgtgcctc tccaagcgca gcagcagctc gacttcgagt gcggtgctcg taagcccgtc
     8761 gagtgtgacg tgcgcatggc gcagcagctc ttcgtcggtg agcctgccca ggttcatggg
     8821 gtttcccttc gtctttggcg ttcggactcg cgcgctcgct gcagcttggc ttccttcttt
     8881 tcgaccgctg cctcgatgcc ctcgacctcg ccgtgcggca gccagccggg cggccagtcg
     8941 cgagccaggg cgagctcggt gaagtacggc agatagggca ccggcaggtc gctcatgccg
     9001 cccaccagtg cagcagcgag agagcgacga cgacgccgag ggccacggcg agcagcacgt
     9061 cggccgtgcg ttcgtgccgg cgccacgggc cactgagcgg cgcgtagtgc gagcgcgagt
     9121 agctccgcga ccaggtgcgg ccggcgagca tgcggcggtg ctcaggcagc gcgttcatgc
     9181 gtcgccctcc tcgatctcgg ccaccagatc gtcgacgtga taggccgcgt gcgctgcggc
     9241 gcgcgccgcg atccacgccg aggcgcgcag gtgcagctcg tgatcggccg cgcgggcggc
     9301 gcacatggcg acgatgcgca gcagctcgtg cagcacctgc tcgccgccct tgctgtcgat
     9361 cgcctcgccg atcacgtcga tggccgtggc tgtggagcgc cgcttgccga agcccggcgt
     9421 actcacctcg cgtgccggat cgagcgtcag ggcgtcgatc agctcgtcgc gcagcgagac
     9481 gtaggcgctc tcggcgagcg cgtcgcggcg ctcctggcgg gcgtagtagc cgcctaggcc
     9541 ggacaccatt gcgttttcga gcgtggcgct cattgcggca ccgccacgaa ctcgccggcc
     9601 tcgttgagcg agtaccaaac atccggctcg accccgttgt cgccgacctt gcttgcgcgg
     9661 atatacctga tggatccatc ggcattgcgg gcgctcagca cgatggcgcc gctggccgca
     9721 gccatggcgc gaccgaaggc gccgcacgcc acggcaacac cccggttgcc ggtggcgctg
     9781 gccgcaccct ggtcgccggt ggcgctggcc gcacccctgt agccggtggc gctggccgca
     9841 ccctggtagc cggtggcgct ggccgcaccc cggtcgccgg tggcgctggc cgcaccccgg
     9901 tagccggcgg ccgacgcagg cgacgccgga tcgatcggca gacagcgctt ggtcgtccac
     9961 tcgatcgccg cgcgcacgag gccagcgatg tcgatctcgg acttgatcgt gatgaggcgg
    10021 ctcgctacct tggtgtcttc gccgtgtcgg ctcaggtcgc cgcgttgctc taccaccgcg
    10081 tagcgcgatt cgctgggtgc gtagtattcc aggacgtcga gcgggtgttc gcacgcgtgg
    10141 aagccgctgg cgcaggcttc gaccttgcca tcgtgctcgt aggtctggcc gatcgcgaac
    10201 tggtagccgc ggcactgcat gttgcggtcg aagcccttgt aggcgacgat ggtctctgtc
    10261 ggctcttgcg tctgcgcagg gtttgcgtct accgccacct cggccttctt cgctgccgcc
    10321 atttcgcttc cctctcgtct ccccttcggc accggcggcg cctggcgtgg ggagagtgca
    10381 cgagacttta gcgtttactg cgtacgtcgt caacagtaaa cactaaacca cgacctcgcg
    10441 caaaccctag gttcgcgctg gcgtgctcgt tggcggcagg tcgcgcagcg ccgccagcgc
    10501 aacccgcagc gcctcttcca gcgttggcgc ggtgggcgcc gtgatcgtgg tagaggcgcc
    10561 ggacgcgact gcggcgccag ggcgggccgt gacgacggtg ccgtcgagcc atcttaccgg
    10621 catgcgcaaa cgccgctcca tgttgcgcgc cgccctagac gcaaagctat agccgttggt
    10681 cacctgcgac aggaaactgg cctgtgaagc ggtcaggtgg aactgctccg tgaccttcgc
    10741 gtgccccccg tgatcggcca cccaagccct caagcgcacg cggcgctcct cctgttcggc
    10801 gtcggttggg tcatccacga gccgaacctt agtcgagact aaagcagtat tcacttgcgc
    10861 actgtctgag taaacactaa agtcggcagc catggacttc ccttcctggc tcgacgccga
    10921 ccgcggccgc ctcacatcgg tggccgaaca cttcggcctc acgcagtcgg ccgtctcgca
    10981 atggcgccag aacggcgtgc cgcccggccg catgaaggca gtgcgcgaac ttaccggcgg
    11041 tgccgtaacg ctcgacgaca tgctgcccga tgccgaccgg ccgcacccgc agggccgccc
    11101 ggccctcgac gtggatgcgc cgaaacacgc cgcgactgcg gtctgagtcg aacggcacct
    11161 gcgcgccgtg acatccactg gcgatccttt cgtggagttg cggggggaga tcctgcgcga
    11221 gcacgtcgac gtgatcgacg cggtcgttca ggccacaccc ggcgcgagcc gcatgagcgt
    11281 gctgcgccgg atcgtggcgg aatgggcgga tcgcaaggtc catgaggcca gtctgattca
    11341 ccgcgtggcg gggaacaacg gaaccgcgcc gcaggcatgc cggcaccgca acggaagcag
    11401 agcggagacg ccgtgagcgt ggtcgcgatc aaaccccact tgaccgccat gtgggccccc
    11461 gacgagctgc gccggctcgt cggctggctc gtctggcgct acgagctgcg cgacggggag
    11521 gccaagccgc gcaaggtgcc gtactacgcc ggcggcggca agcgccacgg cgtgaacggc
    11581 tgccccgaag accgcgcgca gctcgtcacg ttcgacgccg cgtgcgctgc cgcggccaag
    11641 cgcgggttcg acggcgtggg cctggccctg atgcccgagt tcggcgtctg cgtcctcgac
    11701 ttcgatcgct gcctggcggc cgggggcctg caccccgagg tgctcgccct ggtgcaggtc
    11761 acctatgccg agttcagccc gagcggccag ggcgtgcacg ccttcttcac cggcgatctg
    11821 ggcaaccaca aggaccacgg cgagcctttc ggcttcgagg tcttctcgag ccggggcttc
    11881 gtcaccttca ccggcaacag cttgccgcac gtcgagggcc tcgacctgat cgggcccccc
    11941 gtggcgcccg tcaccgacgc cgtcagggcg ctgtgcgcca ggcgcttcgg cccgcgggcc
    12001 gtgcccgacg acgacgaccc gctgatgagc tacagcccgc cgctcgggct gaccgagggc
    12061 cagctgcggg aggccctgga cgtgctggat gcgggcatgc gccacgaccc gtggctgcac
    12121 gtcggcatgg cgctgcacca cgagaccggc ggcgaaggat tcgatctgtg ggaccagtgg
    12181 agcagcaccg cgcccagcta ccccgggcgc gagaacctgc tcaagcgctg ggagtcgttc
    12241 ggccagcgcg caggcaaggt cgtcacggcc cgctcgctcg tcaagctggc caacgagcac
    12301 ggcgcgagga tcaacgtcgc gaccagcctg gccgagttcg acgcaccagc cccgcacaag
    12361 cccgacaggg cgcagcgctt cccgctggtc caggccgcgc agttcgccga cggcgctgcc
    12421 ccgcgctgga tcatcaagga cgtgttaccc caggccgagc tcgtcgtgct tttcggcgag
    12481 agcggtgcgg gcaagtcctt cgccgcgctc gacatggccg gcgcgatcgc gcgcggtgtg
    12541 ccatggcgcg gacacaaggt gcgcaagggc agggtggtct acgtggccgc cgagggctca
    12601 ggcggcatgc gcaagcgcct aaaagcctac gcccggcacc acaacctcga cctggccgac
    12661 atcgagctgc acgtcgtgcc tgcggcgccg aatctgctgc tcaaggaaga cgcgctcgcg
    12721 ctggcgcggg tgatcgccac gctcggcccg gtcgacgtga tcgtgatcga cacgctggct
    12781 cagaccacgc ccggcggcaa cgagaacgcg gccgaggaca tgggccgcgc cctggcgcac
    12841 tgccgcggca tccaccgcgc caccggcgcc gtggtggtgc tggtgcacca cgccggcaag
    12901 gacacgagca agggcgcgcg cggctggtcg ggcttgcgcg ccgcagccga cgccgagctc
    12961 gaggtcgtgc gcctcggcgc ggcccgggtc ctccaggtta cgaaacagaa ggacggtgac
    13021 gaccacgggc gttgggcctt cgacctggtc gaggtggcgc tcggcatgga cgaagacggc
    13081 gacgtggtgt cctcgtgcgt cgtggtcgag gccgcgatgc ctgccaccgg ccaggtgggc
    13141 aagccgctcg ggaagatcga gcaggtggtc aacgaggtga tccaggaatt cgcccaggcg
    13201 cagaccgccg gcatcgaggt cgcagcggtg ctggccgagg cggtgaagcg cctgcccgag
    13261 cctgaggatg gcaagcgcga cacacgtaag cagcacgcca agcgggccct cgcggcgctg
    13321 tgcgagggcg acgaagcggc ctattggatg cgcgacgacg gcactttgga ggtgatgtga
    13381 tgaagcagtc gactacgaac gtgcagggaa tagcgtgcac cacgcttgca ccactgcacc
    13441 acgtgcaaaa aaacgcacgt ggtgcacgtg gtgcaagctt gaaaacgcgg cataccccat
    13501 tgggtatacg agcgtgtggc tccgacgtga gtgtcgtgaa cgcgcaaatt tctgcatgcc
    13561 cgggcgtgaa cgtgcaaaaa aaagctgcac cacgtgcacc acggctgcac cacggtgcac
    13621 cgtggtgcaa ggtgcagtgc tcccaactgc accacggcgg ggggaggggg ggggagcgta
    13681 gctccttccc cccccgccct ggtgcagcag cacggggtgg tggtgggggt atccgacggc
    13741 gggccccttt ggccgaaccc gcaatttttt acgggacacc tggtgcagcg cggacgtgcg
    13801 aatttttgca cgttcgccga gtcgcttcgc cgaggtcccc tccggcgggt cgcaaggcgg
    13861 cgcctggcgc gaccggcgcc gcgcattttt acgaggccgc atgaccggcc gcgtcgactg
    13921 ggagtgcgtg ctggcggact tggcctgggt gctcggcgag ccgatgccgg gcagcgccga
    13981 gcgcacgcct ctgggcacgc gagcgctggc cgagcggctg cgcgtctcgc gtggtgcgct
    14041 gcgcaactgg tgcgccggca ccgagccgcg ccacagcgac gcgttgcggc tgctgcagcg
    14101 ctggtcggag ctcaccggca agcccgcgtc gatggcgccg gtgcggcggg cttgaaatcg
    14161 cccgccactt gtggcgatga tgcgcgacca tgtgcaatcc gctgctcgtc gtcgccgccg
    14221 cgtccgtcgc gtctgccgcc tactcggccg accagcagcg caaggccgtg cactcgcagc
    14281 aagacgcgct cgcggcatcc caagccgagg acgcgcgcaa ggcggccgag gccgagaccg
    14341 gtgcgcaggt cgcagccaat gccaacctgg ccgacgccaa gcggcgccgt cgcacgagcg
    14401 ccctcggcgc aggcgacccc aacgccggca cgagcgacac gctgggcggt acgtcgtcgg
    14461 tgctcg
//
LOCUS       DAOQNN010000035        14286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1741_length_14286_cov_9.876467, whole genome shotgun sequence.
ACCESSION   DAOQNN010000035 DAOQNN010000000
VERSION     DAOQNN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 14286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14286
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1741_length_14286_cov_9.876467"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /gene="flhF"
                     /locus_tag="PKV97_03915"
     CDS             complement(<1..221)
                     /gene="flhF"
                     /locus_tag="PKV97_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585750.1"
                     /note="positive regulator of class III flagellar genes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNB05053.1"
                     /translation="MNVKRYFAPTAREALRALKEALGPDAIVLSNRAVEGGVEILALP
                     GEAVGALQAANRAAVVARAGGEVRPAAVE"
     assembly_gap    425..524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(727..2829)
                     /gene="flhA"
                     /locus_tag="PKV97_03920"
     CDS             complement(727..2829)
                     /gene="flhA"
                     /locus_tag="PKV97_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNB05054.1"
                     /translation="MALNDTLKLRQLLAPANLRPLAAPLLILVILSMMVLPLPVFLLD
                     VFFTFNIAISVMVMLVAMYSTRPLDFSVFPTVLLVTTLLRLSLNVASTRIVLLEGHAG
                     PDAAGKVIEAFGNFLVGGNTAVGLVVFIILTVINFVVITKGAGRIAEVSARFTLDAMP
                     GKQMAIDADLNAGLVDEKEAKRRRKEIAQESDFYGAMDGASKFVRGDAVAGIIILAIN
                     IIGGLFVGIMQHDMAVGDAAHTYTLLTIGDGLVAQIPSLIVSVAAGLVVSRVGDEGDV
                     GGLVIGQLFSNPQVMVLTAAIIGVLGLIPGMPNLVFLLLAATLGGMAWMRRKRIAEEA
                     AAAAAPEAAAQVPMPAADNLEASWNDVSQVDVLGLEVGYRLIPMVDKGQDGELLKRIR
                     GLRKKFAQEVGFLSAPVHIRDNLELKPNGYRLALKGVEVGSGDAYPGQFLAINPGRVS
                     GPLAGRETTDPAFGLPAVWIDASMREQAHALGYTVVDASTVVATHLNHVILNHAAELL
                     GRQETQALLDHLGKESPKLVEDLVPKLLPLGTVQRVLQNLLEEGVNIRDMRSIIEVLA
                     ENAPRVQDPAQLTTRVREALGRAILQGLFPGNAEVQVMALEPGLERILMQAMAAGGEG
                     GAIEPGLADTLLRQTAQIAQRQEDVGLPPVLLVPAQLRLLLSRFLRRAVPSLKVVSNS
                     EVPENRTIRVTAMVGAQG"
     gene            complement(2840..3982)
                     /gene="flhB"
                     /locus_tag="PKV97_03925"
     CDS             complement(2840..3982)
                     /gene="flhB"
                     /locus_tag="PKV97_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNB05055.1"
                     /translation="MAEDSDLEKTEDPSSRRLEQAREEGQVPQSRELSTFLVTMTGAA
                     TLLLLGGWMAGRVMGLFRDGFAFDRRAAFDENVMLDMLERMLGGALLTLMPLFVALMV
                     AAVAAPMVLGGLVFAPKVLGLKFDRLDPLQGLGRMFSLHGLVEMFKAILKSVLVGGVA
                     ALVLWYEFEHLFSLMVEPLETGMADFSATIVFATLLIVLSLGLLAAIDVPFQLWQYHK
                     KLRMTKEEVKRESKEQEGDPMIKGRIRAMQREMARRRMMAEVPKADVVVTNPTHFSVA
                     LKYDAAKMGAPVVVAKGRGELALKIREIAKESEVPLLEAPPLARALYKHCELEQAIPA
                     ALYTAVAEVMAYVYQLDAWMKQGGLPPAAPTALPVPVGMDPGVPAE"
     gene            complement(4190..4657)
                     /locus_tag="PKV97_03930"
     CDS             complement(4190..4657)
                     /locus_tag="PKV97_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNB05056.1"
                     /translation="MKKRSSVLRSSIASVLCAAAFSAAAQGGGASLPVAELGAGMYRI
                     EAEVAHTAEARQTGLMHRVAMPLHRGMVFVFPEEGAHCMWMRNTHLPLSVAFIDARGK
                     ILNIERMAPRTEVNHCAAAPARFALEMNAGWFEERGIAAGALLRGIDRLPAPR"
     gene            complement(4813..5580)
                     /locus_tag="PKV97_03935"
     CDS             complement(4813..5580)
                     /locus_tag="PKV97_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB05057.1"
                     /translation="MATKNLSDRPVLRVEGLCVSYGKVEALTDASITVGEGQIVTVIG
                     PNGAGKTTMLSAIMGLLPSRGRIEFDSVAMVAPEVETMVASGMNLVPEKRELFGEMSV
                     EDNLVLGAFQRYRMGLRDHAQTMEEIFALFPRLRERRTQMAGTLSGGERQMLAVGRAL
                     MAKPKLLMLDEPSLGLAPLITREIFRIVTQLRSRGVSILLVEQNARAALQVADYAYVL
                     ETGGIAMEGPASELAGDRRVIDAYLGVGGKHQEMLAT"
     gene            complement(5580..7394)
                     /locus_tag="PKV97_03940"
     CDS             complement(5580..7394)
                     /locus_tag="PKV97_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HNB05058.1"
                     /translation="MKPALSPRLVMAAFVALLAVAPLVLSAFYVTLLNYIGLYAMVAL
                     GLVLLTGVGGLTSFGQAAFVGLGAYTTALLTTSAELPGWLAWAGGSPWLALLVGLVLT
                     VVVAFLIGKLTLRLSGHYLPLATIAWGLSLYFLFGTMGFLGGHTGLTGIPPISLFGYE
                     LRQGEEVYYLVWLFVLAGVLTTSNLLDSREGRAIRALKGGMVMAEAMGVDTARSRMVI
                     FILAALLACASGWLYAHMQRFVNPTPFGLHIGIEYLFMAVVGGAAHVWGALVGAGVIT
                     VLKQWLQDLLPKLFGTSGNFEVIFFGVLMVLVLHKARGGLWPILVSGFKRFIPVAAQR
                     RAVDRDALALPRRELPAAGSLLLEAKAVTRRFGGLLANNNMSLEVRAGEILALIGPNG
                     AGKSTMFNQVSGVDTPTSGEVRFLGESVVGKGSRTIARMGMSRTFQHVRLLPTMSVLE
                     NVAIGAHMRGTSGVLSAAWRMDRAEEARLLAEAARQIERVGLGEHMFDEAGSLALGQQ
                     RILEIARALASDPCLLLLDEPAAGLRYKEKEALGDLLRKLRGEGMGVLLVEHDMDFVM
                     GLVDRVVVMEFGEKIAEGLPEDVQRDPAVLAAYLGGED"
     gene            complement(7391..8428)
                     /locus_tag="PKV97_03945"
     CDS             complement(7391..8428)
                     /locus_tag="PKV97_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB05059.1"
                     /translation="MDFQIALILGQDGITNGAIYALLALALVLVFAVTRVIFIPQGEF
                     VAYGALTLAMIGNGVFPATAWLLLGAGVAVAALDGLGALRAGQARKLPSVLAVNIGYP
                     LAVLALLRLLPLTELGLAVQILLTMLVVVPLGPMMYRIVFQPIAEATVLVLLIVSVAS
                     HVAMVGLGLLFFGAEGYRTPPLSDASFEVGPLTVSGQTIWVIAASVVLIVALALFFGR
                     TIYGKALRATAINRNGARLMGIPPALAGKLTFLLAALIGVLSGVLISPITTIYYDSGF
                     LIGLKGFVAAIIGGLASYPLAAFGAVLVGLLESFSSFWASAYKEVLVFTLIIPVLVWR
                     SLTSHHVEEEE"
     gene            complement(8543..9691)
                     /locus_tag="PKV97_03950"
     CDS             complement(8543..9691)
                     /locus_tag="PKV97_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB05060.1"
                     /translation="MKLKKTLFVALGLAFAAGAAHAQLKVGITVSATGPAASLGIPER
                     NTVELMPKEIAGQKVTYVVLDDASDTTSAVKNIRKLVSEDKVDVVLGSTTSPNSLGMI
                     DVAAESQTPMIAWAASSRIVEPVDEKRRWVFKTPQNDSHMSTAIVSHMTSNNVKNVAF
                     IGFADAYGEGWYEQFKSVAEARGIKIVANERFTRTDTSVTGQVLKMMAAKPDAMFIAG
                     SGTPAALPQKTLKERGWSGKVYQTHGVANNDFLRVCGADCEGTLLPAGPVLVAEQLPD
                     NNPVKASAMTYIKAYEAAHGKGSVSTFGAHAWDSGVMLQAAVPAALKKAQPGTAEFRA
                     ALRDALENMKEVPGAHGVFTMGPNDHLGLDQRARVMVQIQKGAWKLVQ"
     gene            complement(9878..>10212)
                     /locus_tag="PKV97_03955"
     CDS             complement(9878..>10212)
                     /locus_tag="PKV97_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB05061.1"
                     /translation="VNITSINGLRGKFGQANYTAAKAGMIGLTKTAARELGPKGVTVN
                     AVAPGMVMTEMARALPAEVLERALQESVMRELATPDDIAAAVVFLLSDMARMITGEVI
                     RVDAGQYV"
     assembly_gap    10213..10312
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10313..10743)
                     /locus_tag="PKV97_03960"
     CDS             complement(<10313..10743)
                     /locus_tag="PKV97_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB05062.1"
                     /translation="MGFSGELAGKVAIVTGGARGIGRTITDALVASGARVHVFDMTDS
                     NESAGVLHRVNVADAQSVADAVAQLPEPATLLVNNAGITRDRSLLKMSDEEWRSVLDV
                     NLSGAFHMLRACAPGMIAAGRGRVVNITSINGLRGKFGQAN"
     gene            complement(10819..11364)
                     /locus_tag="PKV97_03965"
     CDS             complement(10819..11364)
                     /locus_tag="PKV97_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB05063.1"
                     /translation="MTTRPHPTTKQLLDQFNTEPDYSDASQDDAEALSRDRIPVRSLT
                     AADFDAVVRIDRRTTGHDRSAYYRRKFDEALGESGVRVSVVAEQDGMVAGFLMARVDF
                     GEFGRAATAAVIDTIGVDAAASGKHVGRALVSQLLANLTSLHVESVRTEVEWNRFDLL
                     RFLDRCGFRPGQQLSLSRPLV"
     gene            complement(11400..11864)
                     /locus_tag="PKV97_03970"
     CDS             complement(11400..11864)
                     /locus_tag="PKV97_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB05064.1"
                     /translation="MTANTEIKLRPLAAGDLAAVTRLDATNGGESRAGFFERRLQAEQ
                     QSPTGFFSCVAERGGEVAGFLLGHLLDGEFGGLERVAVLDAVAIDPAAQRQGLARKLV
                     GEFDRSARERGASEMRTQAQWDRPGLVEFFSAAGFRLSPRLVLERPTEYVNF"
     gene            complement(11923..12165)
                     /locus_tag="PKV97_03975"
     CDS             complement(11923..12165)
                     /locus_tag="PKV97_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNB05065.1"
                     /translation="MALLINDDCTACDACVEECPNEAITAGNPIYVIDPGKCSECVGA
                     FDEPQCKLVCPADCIKDHPDYRESQDELQAKYDSLH"
     gene            complement(12191..13039)
                     /gene="bcrD"
                     /locus_tag="PKV97_03980"
     CDS             complement(12191..13039)
                     /gene="bcrD"
                     /locus_tag="PKV97_03980"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HNB05066.1"
                     /translation="MTITAGIDIGTGAVKTVLFRVEGDKTEWLGKRNDRIRQRDPFKL
                     AEEAYHALLEEAGLKASDVDYVATTGEGESLHFHTGHFYSMTTHARGAVYLNPEARAV
                     LDIGALHGRAIRNDERGKVETYKMTSQCASGSGQFLENIARYLGIAQDEIGSLSNQAD
                     NPEVVSSICAVLAETDVINMVSRGITAPNILKGIHISMAGRLAKLLKSIGAREGVVLC
                     TGGLALDEGLLNTLNESVKEQKMNVTAFNHPDSPFAGAIGAALWGAFRHEKLARLGGQ
                     QLAAAA"
     gene            complement(13042..>14286)
                     /gene="bcrA"
                     /locus_tag="PKV97_03985"
     CDS             complement(13042..>14286)
                     /gene="bcrA"
                     /locus_tag="PKV97_03985"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit A"
                     /protein_id="HNB05067.1"
                     /translation="VLGRGITNSRSNYDTAARVSKLEAFIDARLSLIRRELEKVPAVA
                     GRVDEILAGLTRNFRREQFVEQLGDLEQTCIANVEGPRFAGKEKVIVGALTEVFRRLR
                     EEEADKLFAPTAQRKSDFFRDLAGSRFMQIGEEVARDAGIPFDTLLHMYDKSIIEVEN
                     RPPSGDMNRKFRTAMERVRGEMSSALDTAALGAPIDAALDVDMVERYVVGTGYGRVRL
                     PFPKEHIRSEILCHGLGAHLMYPKTRTVLDIGGQDTKGIQIDEKGIVVNFQMNDRCAA
                     GTGRYLGYVADEMNMGLHELGPLAMKATKSIRINSTCTVFAGAELRDRLALGDKREDI
                     LAGLHRAIMLRAMSIISRSGGITDEFTFTGGVAKNEAAVKELRQLVKENYGEVKINID
                     PDSIYTGALGASEFARRAVVEA"
BASE COUNT         2287 a         4764 c         4873 g         2162 t
ORIGIN      
        1 atctcgaccg ctgccggccg gacctccccg cccgcacgcg ccaccaccgc cgcccgattg
       61 gccgcctgca gcgcgccgac cgcctcgccc ggcagcgcga ggatctccac cccgccttcg
      121 acagcgcggt tggacagcac gatcgcatcc ggaccgaggg cttccttgag cgcgcggagc
      181 gcctcgcggg cggtgggggc aaagtagcgt ttgacgttca tggcgaatct cccggtggcc
      241 gtcgtcgacc actgcttgat gaggattatc gcgaggccgg gtcaagcgcc tggggtggaa
      301 taaagcgggt ttccggggtc aattcgggag acggggagcg cgcaccggcg gcagcgatcg
      361 tggccccgca gccgtgtcgg gaagcgcgag cattgtccgc aggcgcgccg cgagtcgcga
      421 tcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcgca cggaatccgc
      541 tccgacacgg acgaaccgcc tgatcgcgac ttgcgtcgcg cctgcggagg agactccgca
      601 cgcagtggtt cttcgtagga gcgacgcgag tcgcgatcgc ggcgcacgga atccgctccg
      661 acacggacga accgcctgat cgcgacttgc gtcgctccca cggcgactcc gcaggcgcgt
      721 gggtgctcag ccctgcgcgc ccaccatcgc ggtgacgcgg atggtgcggt tctccggcac
      781 ctcgctgttg gagacgacct tcagcgacgg cacggcgcgg cgcaggaagc gcgacagcag
      841 caggcgcagt tgcgcgggca ccagcagcac cggcggcaag ccgacatcct cctggcgctg
      901 ggcgatctgc gcggtctggc gcagcaaggt atcggccagg cccggttcga tcgccccgcc
      961 ctcgccgccc gcagccatgg cctgcatcag gatgcgctcc agcccgggct cgagcgccat
     1021 cacctggacc tcggcattgc ccgggaacag gccctgcagg atggcgcggc cgagcgcctc
     1081 gcgcacccgg gtggtgagct gggccggatc ctgcacgcgc ggcgcgttct cggcgagcac
     1141 ctcgatgatg ctgcgcatgt cgcggatgtt gacgccctcc tcgagcaggt tctgcaggac
     1201 gcgctgcacg gtgccgagcg gcagcagctt gggcacgagg tcctcgacca gcttgggcga
     1261 ttccttgccg aggtggtcga gcagggcctg ggtctcctgg cggccgagca gctcggcggc
     1321 gtggttgagg atgacgtggt tgaggtgggt cgccaccacg gtgctcgcgt ccaccacggt
     1381 gtagccgagc gcgtgcgcct gctcgcgcat cgaggcgtcg atccacaccg cgggcagccc
     1441 gaaggcgggg tcggtggtct cgcgtccggc aagcgggccg gacacgcgcc ccgggttgat
     1501 ggcgaggaac tggccaggat aggcatcgcc actgccgacc tcgacgccct tgagggcgag
     1561 ccggtaaccg ttgggcttga gctcgaggtt gtcgcggatg tgcacgggcg cggacaggaa
     1621 accgacctcc tgggcgaact tcttgcgcag gccgcggatg cgcttgagca gctcgccgtc
     1681 ctggcccttg tcgaccatcg ggatcagccg gtagccgacc tccagcccga gcacgtccac
     1741 ctgcgagacg tcgttccagc tcgcctccag attgtcggcg gcgggcatcg gcacctgcgc
     1801 cgcggcctcg ggcgcggcgg cggccgcggc ttcctcggcg atgcgcttgc gccgcatcca
     1861 ggccatgccg ccgagcgtgg ccgcgagcag caggaagacc aggttgggca tgcccggaat
     1921 caggccgagc acgccaatga tcgccgcggt cagcaccatg acctgcggat tggagaacag
     1981 ctggccgatc accagcccgc cgacatcgcc ctcgtcgccc acccgcgaca ccaccagacc
     2041 ggccgcgacc gagacgatca gcgaggggat ctgcgccacc aggccatcgc cgatggtgag
     2101 cagggtatag gtgtgggcgg cgtcgcccac cgccatgtcg tgctgcatga tgccgacgaa
     2161 gaggccgccg atgatgttga tcgccaggat gatgatgccg gcgaccgcgt cgccgcgcac
     2221 gaacttggac gcaccgtcca tggcgccgta gaagtccgat tcctgcgcga tctccttgcg
     2281 cctgcgcttg gcctccttct cgtcgaccag gccggcgttg aggtcggcgt cgatcgccat
     2341 ctgcttgccc ggcatcgcgt ccagggtgaa gcgcgccgac acctcggcga tgcggcccgc
     2401 gcccttggtg atcaccacga agttgatcac ggtgaggatg atgaacacca ccagcccgac
     2461 cgcggtgttg ccgccaacga ggaagttgcc gaaggcctcg atcaccttgc cggcggcatc
     2521 cggaccggcg tgaccctcga gcagcacgat gcgggtggag gcgacgttga gcgacaggcg
     2581 cagcagcgtg gtgaccagca gcacggtggg aaagacggag aagtccagcg gccgcgtcga
     2641 gtacatcgcc accagcatca ccatcaccga gatcgcgatg ttgaaggtga agaagacgtc
     2701 gaggaggaag accggcagcg gcagcaccat catcgacagg atgacgagga tcagcagcgg
     2761 cgcggcgagc gggcgcaggt tggcaggcgc gagcagctgg cgcagcttga gggtgtcgtt
     2821 cagggccatg gttcaggctt cattccgcgg gcacgccggg atccatcccg acgggcaccg
     2881 gcaaggcagt gggggcggca ggcggcaggc cgccctgctt catccaggcg tcgagctgat
     2941 agacataggc catcacctcg gcgaccgcgg tgtacagcgc ggccgggatc gcctgctcga
     3001 gctcgcaatg cttgtatagc gcgcgcgcaa gcggcggcgc ctcgagcagc gggacttcgc
     3061 tctccttcgc gatctcgcgg atcttgaggg cgagctctcc ccgccccttg gcgaccacca
     3121 ccggcgcgcc catcttcgcg gcgtcgtact tgagggcgac ggagaaatgg gtcgggttgg
     3181 tgaccaccac gtcggccttg ggcacctcgg ccatcatgcg acggcgcgcc atctcgcgct
     3241 gcatcgcgcg gatgcggccc ttgatcatcg ggtcgccttc ctgctccttg ctctcgcgct
     3301 tgacctcttc cttcgtcatg cgcagcttct tgtggtactg ccagagctgg aagggcacgt
     3361 cgatcgcggc gagcaggccg aggctgagca cgatcagcag ggtggcgaag acgatggtgg
     3421 cggaaaaatc cgccatgccg gtctcgaggg gctcgaccat caggctgaac aggtgctcga
     3481 actcgtacca cagcaccagg gcggccacgc cgccgaccag tacggacttc aggatggcct
     3541 tgaacatctc gaccagcccg tgcagcgaga acatgcgccc cagcccctgc agcgggtcga
     3601 ggcggtcgaa cttgagcccg agcaccttgg gtgcgaagac cagaccgccg agcaccatcg
     3661 gcgcagccac tgcagcgacc atcagcgcga cgaagagcgg catcagggtg agcagcgcgc
     3721 cgccgagcat gcgctcgagc atgtcgagca tgacgttctc gtcgaaggcg gcgcggcggt
     3781 cgaaggcgaa accgtcgcgg aacagcccca tcacccgccc cgccatccag cccccgagca
     3841 acagcaaggt cgccgcaccg gtcatggtga cgaggaaggt cgacagctcc cgcgattgcg
     3901 ggacctggcc ttcctcacgc gcctgttcca gcctgcgtga cgaggggtct tctgtcttct
     3961 cgaggtcgct gtcttcggcc acggccggct ccgtccgccg tcgcgccgca ccgcaggaat
     4021 acgggcgcgc ggcgacgtgt gcggattatc ggccgatgcc ggcaagggcc ggacgcagaa
     4081 aacgtggtgg aaagcggcgc aattcggcgc tttgtgcgag cgccgccgag accgcgccga
     4141 tcatcccggc cgtgtcccgg cgcgggcttt cgatccgccc gggtgccctt cagcgcggcg
     4201 cgggcaggcg gtcgataccg cgcagcagcg cacccgcagc gatcccgcgc tcttcaaacc
     4261 agccggcgtt catctcgagc gcgaagcgcg ccggcgcggc cgcgcagtgg ttgacctcgg
     4321 tgcgcggcgc catgcgctcg atgttgagga tcttccccct ggcatcgatg aaggcgaccg
     4381 agagcggcag atgcgtgttg cgcatccaca tgcagtgcgc accctcctcc ggaaacacga
     4441 acaccatgcc gcgatgcagc ggcatcgcca cgcggtgcat cagcccggtc tggcgtgcct
     4501 ctgcggtatg ggcgacctcg gcctcgatgc ggtacatgcc ggccccgagt tcggccaccg
     4561 gcaggctggc gccccccccc tgcgccgcgg cggagaaggc ggccgcgcac aggaccgagg
     4621 cgatcgagga acggagaacg gaggagcgtt tcttcatcgc ggaacaggcc cgaaaaagca
     4681 tggaggccgc gagtttagcg cgcgccgagg agtaaggcgc gagacgtgag gcgcaaggag
     4741 cgaaaccgcc acagccggga agtccgcaag tgcccccgga cccaccgcgc ctcgcacctc
     4801 acgcctcact ccttacgtcg ccagcatctc ctgatgcttg ccgcccacgc cgaggtaggc
     4861 atcgatcacg cgccggtcgc cggcgagctc ggaggcgggg ccctccatgg cgatcccgcc
     4921 ggtctcgagc acgtaggcgt agtcggccac ctgcagcgca gcccgggcgt tctgctcgac
     4981 cagcaggatg gacaccccgc ggctgcgcag ttgcgtgacg atgcggaaga tctcgcgcgt
     5041 gatcagcggc gccagcccca ggctgggctc gtcgagcatc agcagcttgg gcttggccat
     5101 cagcgcgcga ccgaccgcca gcatctggcg ttcgccgccc gagagggtgc cggccatctg
     5161 cgtgcgacgc tcgcgcaggc gcgggaagag cgcgaagatc tcttccatgg tctgcgcgtg
     5221 gtcgcgcaag cccatgcggt agcgctggaa ggcacccagc acgagattgt cttcgacgct
     5281 catctcgccg aaaagctcgc gcttctccgg gaccaggttc atcccgctcg cgaccatggt
     5341 ctcgacctcc ggcgccacca tcgccacgct gtcgaattcg atgcgaccac gcgagggcag
     5401 cagccccatg atcgccgaca gcatggtggt cttgccggcg ccgttggggc cgatcacggt
     5461 gacgatctgg ccctccccga ccgtgatgct ggcgtcggtg agcgcctcga ccttgccgta
     5521 ggaaacgcac aggccttcga cccgcaatac cgggcgatcg ctaaggttct tcgtcgccat
     5581 cagtcttctc ctccgagata ggccgcgagc acggccggat cgcgctgcac gtcctccggc
     5641 aggccctcgg cgatcttctc gccgaactcc atcaccacca cgcgatcgac caggcccatc
     5701 acgaagtcca tgtcgtgctc gaccagcagc acgcccatgc cttcgccgcg cagcttgcgc
     5761 agcaggtcgc cgagcgcttc cttctccttg tagcgcaaac cggcggcagg ctcgtcgagc
     5821 agcagcaggc aggggtcgga ggcgagcgca cgcgcgatct cgaggatgcg ctgctggccg
     5881 agcgcgaggc tgccggcctc gtcgaacatg tgctcgccca ggcccacgcg ctcgatctgg
     5941 cgcgcagcct cggcgagcag gcgcgcctcc tcggcgcggt ccatccgcca cgccgccgac
     6001 agcacgccgc tcgtgccgcg catgtgggcg ccgatggcga cgttctcgag tacgctcatg
     6061 gtgggcagca ggcgcacgtg ctggaaggtg cggctcatgc ccatgcgcgc aatcgtgcgt
     6121 gagcccttgc cgaccaccga ctcgccgagg aaacggactt cgcccgaggt cggcgtatcc
     6181 acgcccgaaa cctggttgaa catcgtgctc ttgcccgcgc cgttgggacc gatcagggcg
     6241 aggatctcgc ccgcccgcac ctccaggctc atgttgttgt tggcgagcag gccgccgaag
     6301 cgccgcgtga ccgccttggc ctcgagcagc aggctgccgg cggccggcag ctcgcgccgc
     6361 ggtagcgcca acgcatcgcg atcgaccgcc cgacgctgtg ccgcgaccgg gatgaagcgc
     6421 ttgaaccccg acaccaggat cggccacagc ccgccgcgtg ccttgtgcag caccagcacc
     6481 atcagcacac cgaagaagat cacctcgaag ttgccgctgg tgccgaagag cttgggcagc
     6541 aggtcctgca gccactgctt gagcacggtg atcacgcccg cgccgacgag cgcgccccag
     6601 acgtgggccg caccgccgac gaccgccatg aacagatact cgatgccgat gtgcaggccg
     6661 aatggcgtcg gattcacgaa gcgctgcatg tgcgcgtaca gccagcccga ggcgcaagcg
     6721 agcaaggcgg cgaggatgaa gatcaccatg cgcgagcgcg cggtgtcgac gcccatcgcc
     6781 tcggccatca ccatgccgcc cttgagtgcg cgaatcgccc tgccctcgcg cgagtcgagc
     6841 aggttgctgg tggtcagcac gccggcgagc acgaagagcc agaccaggta atacacctcc
     6901 tcgccctggc gcaactcgta gccgaacagc gagatcggcg ggatgccggt gaggccggtg
     6961 tggccgccca gaaagcccat ggtgccgaac aggaagtaca ggctcaggcc ccaggcgatg
     7021 gtcgccagcg gcaggtaatg cccggacagg cgcagggtga gcttgccgat caggaaggcc
     7081 accacgacgg tgagcaccag cccgacgagc agggcgagcc agggcgagcc gcccgcccag
     7141 gccagccagc cgggcagttc cgccgaggtg gtgagcagcg ccgtggtgta ggcgccgagg
     7201 ccgacgaagg cggcctggcc gaagctggtc aggccgccca caccggtgag cagcaccagc
     7261 ccgagcgcga ccatggcata caggccgatg tagttgagca gggtgacgta gaaggccgac
     7321 agcaccagcg gcgccaccgc cagcagtgcg acgaacgcgg ccatcaccag gcgcggagag
     7381 agcgcgggtt tcattcctct tcctccacgt ggtggctggt cagggagcgc cagaccaaaa
     7441 ccgggatgat cagcgtgaac accaggacct ccttgtaggc gctcgcccag aacgaactga
     7501 aggattcgag caggccgacc agcaccgcgc cgaaggctgc gagcggatag ctcgccagcc
     7561 cgccgatgat cgcggcgacg aaacccttga ggccgatcag gaagccgctg tcgtagtaga
     7621 tcgtcgtgat cggcgagatc agcacgcccg acagcacgcc gatcagcgcg gcgagcagga
     7681 aggtgagctt gccggcgagc gcgggcggga tccccatcag gcgggcgccg ttgcggttga
     7741 tcgcggtggc gcgcaaggcc ttgccgtaga tcgtgcggcc gaagaacagc gcgagcgcga
     7801 cgatcagcac gaccgaagcc gcaatcaccc agatcgtctg cccgctcacc gtcagcgggc
     7861 cgacctcgaa gctcgcatcc gagagcggcg gcgtgcggta accctcggcg ccgaagaaca
     7921 gcaggcccag gcccaccatc gcgacgtggg acgcgaccga gacgatcagc agcaccagga
     7981 ccgtggcctc ggcgatcggc tgaaagacga tgcggtacat catcggtccg agcggcacca
     8041 ccaccagcat ggtgagcagg atctgcaccg cgaggccgag ttcggttagc ggcaacaggc
     8101 gcagcagggc aaggaccgca agcgggtagc cgatattgac cgcgaggacg ctcggcagct
     8161 tgcgcgcctg cccggcacgc agcgccccga gtccgtcgag ggcggcgacg gcgaccccgg
     8221 caccgagcag cagccaggcc gtggcgggga agacaccgtt accgatcatc gcaagcgtga
     8281 gcgcgccgta ggcgacgaac tctccctgcg ggatgaagat cacccgggtg acagcgaaca
     8341 ccagtaccag cgcgagcgct agcagcgcat agatggcgcc gttggtgatg ccgtcctgac
     8401 cgaggatcag tgcaatctga aaatccatgg acgcgagcct ccgcgcgccc cggccggaag
     8461 ccgaagcgcg atcgagtgga ccgactgatg ggaacggggc ggccgccggg ccgcaagccc
     8521 ggcgaccgcg cgggagcacc gcttactgga cgagcttcca ggcgcccttc tggatctgca
     8581 ccatcacgcg cgcgcgctgg tcgaggccga ggtggtcgtt cggacccatc gtgaacacgc
     8641 cgtgggcccc ggggacctcc ttcatgttct cgagcgcgtc gcgcagcgcc gcacgaaact
     8701 cggcggtgcc gggctgggcc ttcttgagcg ccgcgggaac ggccgcctgc agcatcacgc
     8761 cggaatccca ggcgtgggcg ccgaaggtcg acacgctgcc cttgccgtgc gccgcctcgt
     8821 aggccttgat gtaggtcatc gcgctcgcct tgaccgggtt gttgtcgggc aactgctcgg
     8881 cgaccagcac cggaccggcg ggcagcagcg tgccctcgca gtcggcgccg cagacgcgca
     8941 ggaagtcgtt gttcgccaca ccgtgggtct ggtagacctt gcccgaccag ccgcgctcct
     9001 tgagcgtctt ctgcggcagc gccgccggcg tgcccgagcc cgcgatgaac atggcgtcgg
     9061 gcttggccgc catcatcttg agcacctgcc cggtaaccga ggtgtcggtg cgggtgaagc
     9121 gctcgttggc gacgatcttg atcccgcgcg cctcggcgac cgacttgaac tgctcgtacc
     9181 agccctcgcc ataggcgtcg gcgaagccga tgaaggccac gttcttgacg ttgttgctcg
     9241 tcatgtggga gacgatcgcg gtcgacatgt gggagtcgtt ctgcggcgtc ttgaacaccc
     9301 agcgccgctt ctcgtccacc ggctcgacga tgcggctgga ggccgcccag gcgatcatcg
     9361 gggtctgcga ctcggcggcg acgtcgatca tgccgagcga gttgggcgag gtggtcgagc
     9421 cgagcaccac gtcgaccttg tcctcggaga ccagcttgcg gatgttcttg accgcgctgg
     9481 tggtgtccga ggcgtcgtcg aggacgacgt aggtgacttt ctgcccggcg atctccttgg
     9541 gcatcagctc gacggtgttg cgctcgggga tgccgagcga ggcggccggc ccggtggccg
     9601 atacggtgat gcccaccttg agctgggcat gggcggcgcc cgccgcaaag gcgaggccga
     9661 gcgcgacgaa cagcgtcttc ttaagtttca tggtatctcc tccggtcgtt atggattagg
     9721 cgaccagcct gaccatgacg ggccgccctt cttgccaccc gatgaaaccc gatacctgca
     9781 gttccacgaa aagcgcctca atggtagacg attccgggcc ctgcgggcgt gatgcatgct
     9841 acgcgcaaag cggcttccca ggccgctttg cgatgtttca gacgtactgc ccggcgtcca
     9901 cccggatgac ctcgccggtg atcatgcgcg ccatgtccga gagcaggaag acgacggctg
     9961 cggcgatatc gtccggggtg gcgagctcgc gcatcaccga ctcctgcagc gcgcgctcga
    10021 gcacctcggc cggcagcgcg cgcgccatct cggtcatcac catgccgggc gcgaccgcgt
    10081 tgacggtgac tcccttgggg ccgagttcgc gcgcggcggt cttggtgagg ccgatcatgc
    10141 ccgccttcgc cgcggtgtag ttggcctggc cgaacttgcc gcgcaggccg ttgatcgagg
    10201 tgatgttgac gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntagttggc
    10321 ctggccgaac ttgccgcgca ggccgttgat cgaggtgatg ttgacgaccc ggccgcggcc
    10381 ggccgcgatc atgcccggcg cgcaggcgcg cagcatgtgg aatgcgccgc tcaggttgac
    10441 gtcgagcacc gagcgccatt cctcgtcgct catcttgagc aggctgcggt cgcgcgtgat
    10501 gccggcgttg ttgaccagca acgtcgccgg ctcgggcagc tgcgccaccg cgtcggccac
    10561 cgactgcgcg tcggccacat tcacccgatg cagcacgccc gccgactcgt tgctgtcggt
    10621 catgtcgaag acatgcacgc gcgcgccgga ggcgaccaac gcgtcggtga tcgtgcgtcc
    10681 gatcccgcgc gcgccgccgg tgacgatcgc caccttccca gccagttctc cgctaaaacc
    10741 catgagccgc ttcccctctg gatgtgcata tgcccggagc ccgcttgcgc ggaccccggg
    10801 ccggttgatg ccgagcgctc agacgagcgg gcgcgacagc gagagctgct ggccgggacg
    10861 gaagccgcag cggtcgagga agcgcagcag gtcgaagcgg ttccactcga cctcggtgcg
    10921 caccgactcg acgtgcagcg aggtcaggtt ggcgagcagc tgcgacacca gcgcacggcc
    10981 gacgtgcttg ccgctggccg ccgcatcgac gccgatggtg tcgatcacgg cagcagtggc
    11041 cgcgcggccg aactcgccga agtcgacccg cgccatcagg aagccggcga ccatgccgtc
    11101 ctgctcggcg acgaccgaga cgcggacgcc ggactcgccg agcgcctcgt cgaacttgcg
    11161 ccggtagtag gcgctgcggt cgtggcctgt ggtgcggcgg tcgatgcgca cgacggcgtc
    11221 gaagtcggcc gcggtcagcg agcgcaccgg gatgcggtcg cgcgacaagg cttcggcgtc
    11281 atcctggctg gcgtcgctgt agtcgggctc ggtgttgaac tggtccagca gttgcttggt
    11341 ggtgggatgc ggacgcgtgg tcatcgctga tctccaggtt gtcgtttccg gacgtgaact
    11401 cagaagttca cgtattcggt cgggcgctcg agcaccagcc gcggcgacag gcggaagccg
    11461 gccgcggaga agaactcgac cagaccggga cgatcccatt gcgcctgggt gcgcatctcg
    11521 ctggcgccgc gttcgcgcgc gctgcggtcg aactcgccga ccagcttgcg ggcgaggccc
    11581 tggcgctggg ccgcggggtc gatcgccacg gcgtcgagca ccgcgacgcg ttccaggccg
    11641 ccgaattcgc cgtcgagcag gtggcccagc aggaaaccgg cgacctcgcc gccacgctcg
    11701 gccacgcagg agaagaatcc cgtggggctc tgctgctcgg cctggaggcg gcgctcgaag
    11761 aagccggcgc ggctctcgcc gccattggtc gcgtcgaggc gggtgaccgc cgccaggtcg
    11821 ccggcggcca gcggccgcag cttgatttcg gtattcgcag tcattgcgtc tccctgagat
    11881 gactggggcg gagggccgcc atcggccccc tccgccggtg gatcagtgca ggctgtcgta
    11941 cttggcctgc agttcgtcct gcgattcgcg gtagtccggg tgatccttga tgcagtcggc
    12001 cgggcagacg agcttgcact gcggctcgtc gaaggcaccg acgcactcgg agcacttgcc
    12061 cggatcgatc acgtagatcg ggttgccggc ggtgatcgcc tcgttcgggc actcctcgac
    12121 acaggcatcg cacgcggtgc agtcgtcatt gatgagcagg gccatgatgg cttcctcctg
    12181 taggctggtt tcaggccgcg gcggcgagct gctgcccgcc caggcgcgcg agtttctcgt
    12241 ggcggaaggc accccacagg gccgcgccga tcgcaccggc gaagggcgag tccgggtggt
    12301 tgaacgcagt cacgttcatc ttctgctcct tgaccgactc gttgagggtg ttgagcaggc
    12361 cctcgtcgag cgccaggccg ccggtgcaga gcaccacgcc ctcgcgcgcg ccgatcgact
    12421 tgagcagctt ggccaggcga ccggccatcg agatgtggat gcccttcagg atgttgggcg
    12481 ccgtgatgcc gcgcgacacc atgttgatga cgtcggtctc ggccagcacc gcgcagatgc
    12541 tcgacaccac ctcggggttg tcggcctggt tcgacagcga gccgatctcg tcctgggcga
    12601 tgccgaggta gcgcgcgatg ttctcgagga actgccccga gcccgaggcg cactggctgg
    12661 tcatcttgta ggtctcgacc ttgccgcgct cgtcgttgcg gatcgcccgc ccgtgcaacg
    12721 cgccgatgtc gagcaccgcg cgcgcttccg ggttgaggta gaccgcgcca cgcgcatggg
    12781 tcgtcatgga gtagaagtgg ccggtgtgga agtggaggct ctcgccctcg ccggtggtgg
    12841 cgacgtaatc cacgtcggac gccttgaggc cggcctcctc gaggagcgcg tggtaggcct
    12901 cctcggcgag cttgaaggga tcgcgctgac ggatgcggtc gttgcgcttg cccagccact
    12961 cggtcttgtc gccctcgacg cggaagagga cggtcttgac ggcgccggtg ccgatgtcga
    13021 tgcctgcggt gatggtcatt ttcaagcctc cacgacggcg cgacgggcga attcggaagc
    13081 cccaagcgca ccggtataga tcgaatcggg gtcgatgttg atcttcacct ccccgtagtt
    13141 ttccttgacg agctggcgca actccttgac cgcggcctcg ttcttggcca cgccgccggt
    13201 gaaggtgaac tcgtcggtga tgccgcccga gcgcgagatg atcgacatcg cgcgcagcat
    13261 gatggcgcgg tgcaggccgg cgaggatgtc ctcgcgcttg tcgcccagcg ccaggcggtc
    13321 gcgcagctcg gcgccggcga acacggtgca ggtcgagttg atgcggatcg acttggtcgc
    13381 cttcatcgcg agcgggccga gttcgtgcag gcccatgttc atctcgtcgg cgacgtagcc
    13441 cagatagcgg ccggtgccgg cggcacagcg gtcgttcatc tggaagttca ccacgatgcc
    13501 cttctcgtcg atctggatgc ccttggtgtc ctggccgccg atgtcgagca ccgtgcgggt
    13561 cttcggatac atcaggtggg cgcccaggcc gtggcagagg atctcggagc ggatgtgctc
    13621 cttcgggaag ggcaggcgga cgcggccgta gccggtgccg accacatagc gctcgaccat
    13681 gtccacatcg agcgccgcat cgatcggcgc gccgagcgcg gcggtgtcga gggcgctgga
    13741 catctcccca cgaacccgct ccatcgcggt gcggaacttg cggttcatgt cacccgaagg
    13801 cggacggttc tcgacctcga tgatcgactt gtcgtacatg tgcagcaggg tgtcgaaggg
    13861 gatcccggca tcgcgcgcca cttcctcacc gatctgcatg aagcgcgaac cggccagatc
    13921 gcggaagaag tccgatttgc gctgcgccgt gggtgcgaac agcttgtcgg cttcttcctc
    13981 gcgcaggcgg cggaagactt cggtcagcgc accgacgatc actttttcct tgccggcgaa
    14041 gcgcgggcct tcgacgttgg cgatgcaggt ctgctcgagg tcgccgagct gctcgacgaa
    14101 ctgctcgcgg cggaagttgc gcgtcaggcc ggcgaggatc tcgtctaccc gaccggcgac
    14161 ggccggcacc ttctcgagct cgcgacggat caggctcagg cgcgcatcga tgaacgcctc
    14221 gagcttggag acgcgtgcgg cggtgtcata gttcgagcgc gagttggtga tgccgcgacc
    14281 gagcac
//
LOCUS       DAOQNN010000036        13798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1861_length_13798_cov_8.318489, whole genome shotgun sequence.
ACCESSION   DAOQNN010000036 DAOQNN010000000
VERSION     DAOQNN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 13798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13798
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1861_length_13798_cov_8.318489"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PKV97_03990"
     CDS             <1..832
                     /locus_tag="PKV97_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNB05068.1"
                     /translation="TTIIETGINIPTANTIIINRADRFGLAQLHQLRGRVGRSHHQAY
                     AYLLTQPGAKPSAQAQKRLEAIAMMEDLGSGFYLAMHDLEIRGAGEVLGENQSGEIQQ
                     VGFSLYTEMLKRAVKDLQAGKEPDLSQPLEVVSEINLHTPALLPTDYCPDVQERLTLY
                     KRLANCEMEEDLRALQEELIDRFGELPAQTAALIETHRLRMLVKDYGVHKLDASDAQI
                     SVQFAKDAPIDPVKVIFLIQKDRSTKMAGPEKLVRRASLPNLKERVKAVKDLLEAVKA
                     "
     gene            862..1827
                     /gene="corA"
                     /locus_tag="PKV97_03995"
     CDS             862..1827
                     /gene="corA"
                     /locus_tag="PKV97_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNB05069.1"
                     /translation="MLINCIAYQHGRKLADIPVEDISEYIQQPETFVWVALKDATAEE
                     LAVMKEEFGLHELAVEDANHRHQRPKVEEYDDELFVVMHLIEEREGKLEVGEVHVFVG
                     TNYILSVRNNHARDFLGVRARCEREPKMLAKGPGYVLYALMDAVVDGYFPLIDKLETE
                     VEALEERIFTKGAGNTNMRRLYRLKRKVTLLKHAVLPLMEAAGKLHSGRVPEVCAKSR
                     HYFRDVYDHLARIDASLDSVRETIGTAIQVNISLVTIDQTEVSKRLAAWAGIFAVATA
                     FAGIWGMNFNHMPELEWEYGYFAGLGIIVSASTFLWWRFRKARWL"
     gene            2312..3649
                     /locus_tag="PKV97_04000"
     CDS             2312..3649
                     /locus_tag="PKV97_04000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNB05070.1"
                     /translation="MSFFDDLARSLERNRITRRQALWLLGAGAAAGLSGCATSPVTGE
                     TILVGMSEAQEKQTDAQVAPHQFSQDLGAIQDEAVNRYVAGIGQRMGALTHRPQMPYS
                     YRVLNANYVNAYTFPGGAMGVTRGILADLDDEAQLAALLGHELGHVNARHAAQRQGQN
                     LVAQAALAGLNVAAQSSDWGGLMSMGGQIGASALLAGYSREHEREADALGQEYLVKAG
                     YPATGMVRLHQLLVAEEKSAPSLLQTMFSTHPMSSERMQAAQAAADARYRISNSLDAR
                     RERFMDSTASLRRIRPTIDACKNGETAMAARQYPKAQAEFQTALARTPRDYASNLRMA
                     QCLQAQGQTAKAVDYADNAREIYPQEAQAYKLAGVLALQQRDAGRAYQNLDRFDRLVP
                     GDAGITFLKGISLEGMGNRQAAAQHYAAYLRQSQQGNAAQYSYNRLKAWGMVK"
     gene            complement(3694..5091)
                     /gene="ccoG"
                     /locus_tag="PKV97_04005"
     CDS             complement(3694..5091)
                     /gene="ccoG"
                     /locus_tag="PKV97_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNB05071.1"
                     /translation="MNPPVSKVREAKLEFYRKKGKIHAKAVQGRFNGLRWAMVWLTQV
                     VFYGACWLPWTTEAGTRQAILFDIVDEKFYLFDLVLWPQDALLLAVVLILAATALFFT
                     TAIAGRLFCGFACPQTVYTSIFVWIEARIEGDHLARLRLDQGPMSARKLLIKSVKHGI
                     WALVAAWTAISFVGYFTPIRALLPAIVGLQTGPWETFWLLFYASFTYLQAGLAREAVC
                     QHMCPYSRFQGVMIDPDTANVAYDVKRGEPRGALRQSGDKGDCIDCGICVQVCPTGID
                     IRDGLQYQCIDCGLCADACDEVMDRIGAPRGLIRFMSERELAGQAQVAPAWSRPRTLV
                     YVGLLLVFAALGVWTLSERVLLRVEVHRDRGALMQETSAGRIENSYTLKLINMEETTR
                     QFRIGVSGLPGLRIEGAGEFTVGPGQVQSVTLTLSSPFEGMPSGVQPIVFETVALHDA
                     SLAVREKGTFILPFR"
     gene            5306..5899
                     /locus_tag="PKV97_04010"
     CDS             5306..5899
                     /locus_tag="PKV97_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HNB05072.1"
                     /translation="MSLPTTAGDAPPCTGRVPGNKGIWVGICCEFVEFLVLFIVYFVA
                     RAHFPDAFHTGAERLSQTAGVVITLLMVSSSFFIACSVTRLRTGRRRESIAWLIAGLI
                     VALGYPVAKYLEIQWNLAHGIDGESGIFFTVYYYLTFTHMVHAFWGILGILWVLARHG
                     FGIYTADEHSGLEALASYWHATDIIWLVIFPFFYVLA"
     gene            5902..6210
                     /locus_tag="PKV97_04015"
     CDS             5902..6210
                     /locus_tag="PKV97_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05073.1"
                     /translation="MDSIERKPATSLRPLVLTWLALAGLTLVSPQLGKGVWGQEAVQW
                     IVAAIIWIKGHLVARHFIEAERTHPFLRNVLRFFIAFTPIAIVLTTFFRDGFARLASL
                     "
     gene            complement(6205..7617)
                     /locus_tag="PKV97_04020"
     CDS             complement(6205..7617)
                     /locus_tag="PKV97_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNB05074.1"
                     /translation="MSLYQDFADRTAQRIAEGVLRPGDKLLSVRQACKTHGISPITVT
                     QAYHLLESRGLIEARPKSGYFVRARLGSRLPEPEMTHPAGGSTALEVSDFIFQILDSV
                     RDPAIVPLGSSFPDARLYPLDKLGRFLAAAARHLDPLATVTDLPPGNDELRRQLALRY
                     LAHGASVSPQEIVVTSGAMEGLNLCLQALTRPGDLIAIESPTFYAGLQASERLGLEVI
                     EIPSHPREGVSLDALADALRHHPIKACLFMLNFANPTGSRVPDDNKRALVELLHQHQV
                     PLIEDDVYAELYFGPEAPRCSKAMDTEGLVLHVSSFSKCLAPGYRLGWVAAGRYAKEV
                     QRQKYSTSLATAVPMQIALADYLKHGGFDNHLRHLRRQLARQEAQLLAGIEEHFPPGI
                     RLARPDGGYFLWLELPPRIDTLRLHEEALAHGVSIAPGPIFSAKREFSNYLRLNFGHP
                     ATTRQDDALATLGRLIKRQL"
     gene            complement(7756..8355)
                     /locus_tag="PKV97_04025"
     CDS             complement(7756..8355)
                     /locus_tag="PKV97_04025"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNB05075.1"
                     /translation="MSERCAWCGTDPLYVAYHDTEWGVPNHDDRHLFEMLVLEGAQAG
                     LSWITILRKREHYRRAFDGFDAERVARYGEADLARLLADAGIVRNRLKIESAVANARA
                     TLAAQERFGSLDALLWRFAEGSVPTAPPRSLAEIPAQTAASQAMSRELKRLGFRFVGP
                     TVCYAFMQAVGMVNDHVEGCFRQREIAAQRCVTSPPPRP"
     gene            8623..11253
                     /gene="cas3"
                     /locus_tag="PKV97_04030"
     CDS             8623..11253
                     /gene="cas3"
                     /locus_tag="PKV97_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HNB05076.1"
                     /translation="MRHNLSEAFAFGKLDRSEDAIKWHPLVDHLADVAAVFEALCACR
                     AIRRSIEAVAGRSLDHRDFARLAALAFLHDLGKANAGFQAKRWLNEERPRGRLTAGHS
                     AEAIALLEASNHSDEAEALLLHLPILEICGWGKPETLDNLLRASIAHHGRPIANAPDW
                     FNARIHWSRQGEYDPAEQLKSIGAALQRFVPEAFVEGGLPLPDTPRFAHYFAGLVQLA
                     DWLGSDTRFFPFTNVGDRDRIGNSRELASTAVREIGLDPEPCRKRFEQASPDFDDVFD
                     TTTPYPAQSAMSDQTLGQVVVLEAETGSGKTEAALWRYLHLFARGEVDGLYFALPTRV
                     AASQAYRRVREALSRAWPDGAPLAVRALAGYAAADGETARALPDFKVLWSDDPSDAEA
                     GRRWAGESSKRFLAAPVAVGTVDQALLGTLQVKHAHLRHALTARSLLVIDEVHASDAY
                     MGVLIEQLIDAQIALGGHTLLLSATLGAAARTRYLAAGNRRAQRLPSLAQASCLPYPA
                     ISDAAGLHATSGAPRAKQVAWQCEDAIDDPQRVAALALEAAEQGARVLVIRNTVPAAV
                     ATLAALEAATPNAAWLFNCDGVITLHHSRFSREDRPVLDATVEAQLGKGRPSGPLIVV
                     GTQTLEQSLDLDADFLITDLCPMDVLLQRIGRLHRHMRPVEARPNAFREARVLVLTPN
                     AGNLAPCLTRPRHGLGRFRDGGGVYADVRILEATRKLIHAESQVRIPADNRRLVEFAT
                     HPEALHAIESMGTAWATHGQQVDGDTGARRTSAKLGLLPLDTAFVELGFPDQVKLATR
                     LGAADRLLTFDPPLQGPFGQAVRELPIRHHLLPRALPLEALPEDIVVEHGGFRFRLAD
                     THYRYSRIGLEKIPQTAASE"
     gene            11257..12837
                     /locus_tag="PKV97_04035"
     CDS             11257..12837
                     /locus_tag="PKV97_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HNB05077.1"
                     /translation="MDRPHLNLLDEPLIRVRNLGGQTQRLTLPQLLAALGRDAVRDYP
                     ALRPHQRHPWHALLVQLAALALHAADQERPWDEATDWRAALLALTPDHPDGCAFCLVA
                     PHDRPAFLQPPVPSGKLDGWKYIATPDALDMLVTSKNHDLKSERMRHAEADDWLFALA
                     SLQTQEGFLGKGNYGISRMNGGFSSRPSLGVTPAGGVGVRWRRDIGALLTGRDDITET
                     IGFQRDGGMALLWIPPWDGTKSLAFTALDPFYIEICRRIRLVLDSDGRLKARGIGTSA
                     ARVDAAARKGVTGDAWTPVEDDKALTLGSDGFNYSLAAELAWGSKYRKTLAQALRAED
                     GTAGISLLAQGVVRGQGKTEGYHERRIPLTKKAIGYLRGGQTDLPAAIAAARVKAVAT
                     MRGSILRTALFYLLEAGTEKIDFDRRTAKQQIEAFIRTFERTEDARFFEDLNREIEAE
                     DRNAERLAWMLGLAERAEVVLRGAFTIGPQSGERRYRARSRALSYFHGALRSGKHPEL
                     VALAQHFRSYQAAEEAAS"
     gene            12834..13364
                     /locus_tag="PKV97_04040"
     CDS             12834..13364
                     /locus_tag="PKV97_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HNB05078.1"
                     /translation="MNQTDTTPPASPLGRLAQVITRRQHFRGDASALDTGEQAALARL
                     DPEAPRPHQVGALAHALVLAGIKPDDWQHTTWRRWALLAQGMALAGHDGSARLGTQLA
                     HAAVSESRVTRLLTARGDAFSQQVPRVVRLMASRGVSPNWLEFGALILATDHDEARAE
                     TLRLRIAGNYYSTTHS"
     gene            13403..>13798
                     /locus_tag="PKV97_04045"
     CDS             13403..>13798
                     /locus_tag="PKV97_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HNB05079.1"
                     /translation="MSLPRFIQIHTLHTYPAALLNRDDAGLAKRLPYGGAIRTRISSQ
                     CLKRHWRVADDAFSLAKLGVPMATRTRYVAELIRQRLIEQGIEEARAFATAEALLEAL
                     FGEKADKKKEGAKALQTGQAVLFGNEEIAY"
BASE COUNT         2468 a         4764 c         4306 g         2260 t
ORIGIN      
        1 caccaccatc atcgagaccg gcatcaacat ccccaccgcc aacaccatca tcatcaaccg
       61 cgccgaccgc ttcggcctgg cgcagctgca ccagctgcgc ggccgcgtcg ggcgcagcca
      121 ccaccaggcc tacgcctacc tgctgaccca gccgggcgcc aagcccagcg cgcaggcgca
      181 aaaacggctc gaagccattg cgatgatgga agacctcggc tccggcttct acctggcgat
      241 gcacgacctc gagatccgcg gcgccggcga ggtgctgggc gagaaccagt cgggcgagat
      301 ccagcaggtc ggcttcagcc tgtacaccga gatgctcaag cgtgcggtca aggacctgca
      361 ggccggcaag gagcccgacc tgtcgcagcc gctggaggtc gtctccgaga tcaacctgca
      421 caccccggcg ctgctgccca ccgactactg ccccgacgtg caggagcgcc tgacgctgta
      481 caagcgcctg gccaactgcg agatggaaga agatttgcgt gcgctgcagg aagagctcat
      541 cgaccgcttc ggcgagctcc cggcgcagac cgccgcgctg atcgagaccc accgcctgcg
      601 catgctggtg aaggactacg gggtgcacaa gctcgatgcg tccgatgcgc agatcagcgt
      661 gcagttcgcc aaggacgcgc cgatcgaccc ggtcaaagtc atcttcctga tccagaagga
      721 ccgcagcacc aagatggccg gccccgagaa gctcgtgcgc cgtgccagcc tgcccaacct
      781 caaggagcgc gtgaaggcgg tgaaggacct gctcgaggcg gtgaaggcct gatccggcga
      841 aggcgcaagc gacccacgac catgctcatc aactgcatcg cctaccagca cggccgcaag
      901 ctcgccgaca tcccggtcga ggacatcagc gagtacatcc agcaacccga gaccttcgtc
      961 tgggtcgcgc tcaaggacgc caccgccgag gaactcgcgg tgatgaagga ggagttcggc
     1021 ctccacgagc tcgccgtcga ggacgccaac caccgccacc agcgccccaa ggtcgaggag
     1081 tacgacgacg agctcttcgt cgtcatgcac ctgatcgagg agcgcgaggg caagctcgag
     1141 gtcggcgagg tgcacgtctt cgtcgggacc aactacatcc tgtcggtgcg caacaaccac
     1201 gcccgcgact tcctcggcgt gcgcgcacgc tgcgagcgcg agccgaagat gctcgccaag
     1261 ggcccgggct acgtgctgta cgcgctcatg gacgcggtgg tcgacggcta cttcccgctc
     1321 atcgacaagc tcgagaccga ggtcgaggcg ctcgaggagc gcatcttcac caagggtgcg
     1381 ggcaacacca acatgcgccg cctctatcgc ctcaagcgca aggtcaccct gctcaagcac
     1441 gccgtgctgc cgctgatgga ggccgccggc aagctgcaca gcgggcgtgt gcccgaggtc
     1501 tgcgccaaga gccggcacta cttccgcgac gtgtatgacc acctcgcccg tatcgacgcc
     1561 tcgctcgaca gtgtccgcga gaccatcggc accgcgatcc aggtgaacat ctcgctggtc
     1621 accatcgacc agaccgaggt cagcaagcgc ctcgcggcct gggccggcat cttcgcggtg
     1681 gccaccgcct tcgcggggat ctggggcatg aacttcaacc acatgcccga gctggagtgg
     1741 gagtacggct acttcgccgg cctcggcatc atcgtcagcg ccagcacctt cctgtggtgg
     1801 cgcttccgca aggcaaggtg gctgtagcgc gcagccgatg cgtaggagcg acgcaagtcg
     1861 cgattgccgc cgctccgccc cgccgacgcc ccttcccgct ccaccgcccg atcgcgactc
     1921 gcgtcgctcc tacaaatccc cggcacgccg gccgatcgcg actcgcgtcg ctcttccaaa
     1981 ttaccgcttg gcgtgccgta ggagcgacgc aagtcgcgat tgcagcgcct ccgtccccat
     2041 caacgcccat cgtagcccag ccgcccgatc gggactcgcg tcgctcctgc aggagggccg
     2101 cgacgcggtc gggtgcggcc gatcttgtcg gagcgacgcg agtcgggtgc ggcgggtctt
     2161 gtgggagcga cgcgagtcgg gtgcggcggg tcttgtggga gcgacgcgag tcgcgattca
     2221 gaccctccgc cctcgccctc gcccctgccc tgccccagac ctttcctccg cgccgctaca
     2281 atccccgcat gtccttcgac gagcgaatgc catgagcttc ttcgacgacc tcgcccgcag
     2341 cctcgaacgc aaccgcatca cccgccgcca ggcgctgtgg ctgctcggcg cgggcgcggc
     2401 ggccggcctg tcgggctgcg ccacctcccc ggtcaccggc gagaccatcc tcgtcggcat
     2461 gagcgaggcg caggagaagc agaccgacgc acaggtcgcg ccgcaccagt tctcgcagga
     2521 cctcggcgcc atccaggacg aggcggtcaa ccgctacgtc gccggcatcg gccagcgcat
     2581 gggcgcgctc acccaccgtc cgcagatgcc ctactcctac cgcgtgctca acgccaacta
     2641 cgtcaacgcc tacaccttcc ccggcggcgc gatgggcgtg acgcgcggca tcctcgccga
     2701 cctcgacgac gaggcccagc tcgccgccct gctcggccac gagctcggcc acgtcaacgc
     2761 ccgccacgcc gcccagcgcc agggccagaa cctggtcgcg caggcggcgc tcgccgggct
     2821 caacgtggcg gcacagagct ccgactgggg cgggctgatg agcatgggcg gacagatcgg
     2881 cgccagcgcc ctgctcgccg gctactcgcg cgagcacgag cgcgaggctg acgcgctcgg
     2941 gcaggaatat ctcgtcaagg ccggctaccc ggcgaccggc atggtgcgcc tgcaccagtt
     3001 gctggttgcc gaggaaaaat ccgccccctc gctgctgcag acgatgttct ccacccaccc
     3061 gatgagcagc gagcgcatgc aggccgcgca ggccgcggcc gacgcgcgct accgcatcag
     3121 caacagcctg gacgcccgcc gcgagcgctt catggacagc accgccagcc tgcgccgcat
     3181 ccgccccacc atcgacgcct gcaagaacgg cgagaccgcg atggccgcca ggcagtaccc
     3241 caaggcgcag gccgaattcc agacggcgct ggccaggacc ccgcgcgatt acgccagcaa
     3301 cctgcgcatg gcccagtgcc tgcaggccca gggccagacc gcgaaggcgg tggactacgc
     3361 cgacaacgcg cgcgagatct acccgcagga ggcgcaggcc tacaagctcg ccggcgtgct
     3421 cgccctgcag cagcgcgacg ccggccgcgc ttaccagaac ctcgaccgct tcgaccgcct
     3481 ggtccccggc gacgccggca tcaccttcct gaagggcatc tcgctcgaag gcatgggcaa
     3541 ccgccaggcc gccgcccagc actacgccgc ctacctgcgc cagagccagc agggcaacgc
     3601 cgcgcagtac tcgtacaacc ggctcaaggc ctgggggatg gtgaagtagc gcaggatcag
     3661 cccgccgcga tcccgcgcct gcgagccacc atttcaacgg aagggcagga tgaaggtgcc
     3721 cttctcacgc accgcaaggc ttgcgtcgtg cagcgccacc gtctcgaaca cgatcggctg
     3781 cacgccggac ggcatgcctt cgaacgggga tgacagggtg agcgtgacgc tctgcacctg
     3841 gcccggcccg accgtgaatt cgccggcgcc ctcgatccgc agcccgggca ggccgctcac
     3901 gccgatgcgg aactggcggg tcgtctcttc catgttgatg agcttgaggg tgtagctgtt
     3961 ctcgatccgg cccgcgctcg tctcctgcat gagggcgccg cgatcccggt gcacttccac
     4021 ccgcaacagc acgcgctcgc tcagtgtcca caccccgagc gcggcgaaca cgagcagcag
     4081 gccgacatag accagggtcc gcggccgcga ccaggctggg gcaacctgtg cctggccggc
     4141 aagctcgcgc tcgctcatga agcggatcaa gccgcgcggt gcgccgatcc tgtccatgac
     4201 ctcgtcgcaa gcgtcggcgc acaacccgca gtcgatgcac tggtattgca gtccgtcgcg
     4261 aatgtcgatg cccgtcggac acacctgcac gcagattccg cagtcgatgc agtcgccctt
     4321 gtcgccggac tgccgcaagg cgccgcgggg ctccccgcgc ttgacgtcgt aggcgacgtt
     4381 cgccgtatcc ggatcgatca tcacgccctg gaagcgcgag tacggacaca tgtgctggca
     4441 tacggcctcg cgtgcgagcc cggcctgcaa ataggtgaag ctcgcgtaga agagcagcca
     4501 gaaggtttcc cacgggccgg tctggaggcc gacgattgcc ggcagcagcg cgcggatggg
     4561 agtgaagtag ccgacgaagc tgatcgcggt ccacgccgcg accagcgccc agatgccgtg
     4621 cttgaccgac ttgatgagca gcttgcgcgc cgacatcggg ccctggtcga ggcgcaggcg
     4681 cgcgaggtgg tcgccctcga tcctggcctc gatccacacg aagatcgacg tatagaccgt
     4741 ctgcggacag gcaaagccgc agaacaggcg cccggcgatc gcggtggtga agaacagcgc
     4801 ggtcgcggcc aggatcagga cgacggccag cagcagcgcg tcctgcggcc acaggaccag
     4861 gtcgaacagg tagaacttct cgtcgacgat gtcgaacagg atcgcctgcc gcgtgccggc
     4921 ctccgtcgtc cacggcaacc agcacgcgcc gtagaacacg acctgggtga gccagaccat
     4981 ggcccagcgc aggccgttga agcggccctg caccgccttg gcgtggatct ttcccttctt
     5041 gcgatagaac tccagcttgg cttcgcgcac cttgctgacg ggtgggttca tgcgggctgc
     5101 tccggctctg acgatggcct ggagtacatc accccggacg gcgcaggttc agttgcagat
     5161 cgtacgattt ggaacaggta cagatccgcc gccacagctc gtctgtgccg gacgactatc
     5221 cactcgactg ttcctgtccg ctcatctgca aacgcccaaa atcgcgctat cgaatcagga
     5281 gtggaacacc gtgatcgcca atacgatgag tttgcccacc accgcgggtg acgcgccccc
     5341 ctgcaccggc agggtgcccg gcaacaaggg gatctgggtc gggatctgct gcgagttcgt
     5401 cgagttcctc gtgctgttca tcgtgtactt cgtcgcccgc gcgcactttc ccgacgcctt
     5461 ccacaccggg gcggagcggc tctcgcagac ggccggcgtg gtgatcacct tgttgatggt
     5521 gagcagcagc tttttcatcg cctgctcggt cacccgcctg cgcaccgggc gcaggcgcga
     5581 atcgatcgcc tggctgatcg cgggcctgat cgtggcgttg ggctatccgg tggcgaaata
     5641 cctcgagatc cagtggaacc tcgcgcacgg tatcgatggc gagtcgggca tcttcttcac
     5701 cgtctattac tacctgacct tcacccatat ggtgcacgcc ttctggggca tcctcggcat
     5761 cctctgggtc ctcgcccgac acggattcgg aatctatacc gccgacgagc acagcggtct
     5821 ggaagcgctc gccagctact ggcacgcgac tgacatcatc tggctggtca tctttccctt
     5881 cttctacgtg ctggcctgat catggactcc atcgagcgca aacccgccac ttcactccgg
     5941 cccctggtcc tgacctggct ggccctggcc gggctgaccc tcgtcagccc gcaactcggc
     6001 aagggtgtct gggggcagga agccgtccag tggatcgtgg ccgcaatcat ctggatcaag
     6061 ggccacctgg tcgcgcgcca cttcatcgag gcggaacgga ctcacccctt ccttcgcaac
     6121 gtgctgcggt tcttcatcgc cttcacgccg atcgcgatcg tgctgacgac cttcttccgc
     6181 gacggcttcg cccgcctcgc gagcctatag ctggcgcttg atcagccggc cgagcgtcgc
     6241 cagcgcatca tcctggcgag ttgttgcggg atggccgaag ttgagccgca ggtaattgga
     6301 gaactcgcgc ttggcggaaa agatcggccc cggtgcgatg ctgaccccgt gcgcgagcgc
     6361 ctcttcgtgc agccgcagcg tatcgatccg aggcggcagt tcgagccaga gaaaatagcc
     6421 gccgtccgga cgggccaggc ggataccggg gggaaagtgt tcttcgatgc cggccaggag
     6481 ttgtgcttcc tggcgcgcaa gttgccggcg caagtggcgc aggtgattgt cgaagccgcc
     6541 atgcttcagg tagtcggcca gcgcgatctg catggggacc gccgtcgcca ggctggtcga
     6601 gtatttctgc cgctgcacct ccttggcgta acgccccgcc gcgacccaac ccaagcggta
     6661 tcccggcgcg aggcacttcg aaaacgagga cacgtgcagc accagaccct ccgtgtccat
     6721 cgccttgctg catcgcggcg cctcgggtcc gaaatacaac tcggcgtaga catcgtcttc
     6781 gatcagcggg acctgatgct ggtgcagaag ctcgaccagc gcacgcttgt tgtcgtccgg
     6841 gacgcgactt cccgtcgggt tcgcgaagtt caacatgaac aggcaggcct tgatcggatg
     6901 atggcgcagc gcatcggcca gcgcatccag gctcacccct tcgcgcgggt ggctcgggat
     6961 ctcgatgacc tcgagtccca ggcgctcgct ggcctgcagc cccgcataga aggtgggcga
     7021 ctcgatggcg atcaggtcgc cgggtcgggt cagggcctgc aggcagaggt tgagcccttc
     7081 catcgccccc gacgtgacga cgatctcctg cggcgaaacc gaggcgccat gggccaggta
     7141 acgcagcgcg agttgtcggc gcagctcgtc gttgcccggg ggaaggtcgg tgaccgttgc
     7201 cagcggatcc aggtgacgcg ccgctgccgc gaggaagcgc ccgagcttgt cgagcgggta
     7261 gaggcgcgcg tcgggaaagc tcgaccccaa cggcacgatg gcgggatcgc gcacgctgtc
     7321 gaggatctgg aagatgaagt cgctgacctc gagcgccgtg gagccgcccg ctggatgggt
     7381 catttccggc tcgggcagtc tgctgccaag ccgggcgcgc acgaagtagc ccgacttcgg
     7441 ccgcgcctcg atcaggcctc ggctctcgag caggtggtag gcctgggtga ccgtgatcgg
     7501 gctgatgccg tgggtcttgc aggcctggcg gacggagagc agtttgtcgc ccgggcgcaa
     7561 gacaccctcg gcgatccgct gtgcggtccg gtcggcaaaa tcctggtaga ggctcatggg
     7621 gacgatccta gcgatgcgca gcgaacccgg ccttgccatg gatcacttcg atcgctgccg
     7681 cttcgcaagc aggcgatgcc gcctggctgc gccagagcct gcacggcaac accgcgaggc
     7741 attcctgcaa ccggctcaag gcctgggggg tggtgaagtc acgcagcgct gcgccgcgat
     7801 ctcgcgctgg cggaagcacc cctccacatg gtcattgacc atccccaccg cctgcatgaa
     7861 ggcatagcac accgtcggcc ccacgaagcg gaagcccaag cgcttcagct cacggctcat
     7921 cgcctgcgac gccgccgtct gcgccggaat ctccgccagg ctgcgcggcg gcgcggtcgg
     7981 caccgaaccc tcggcgaagc gccacagcag cgcatccagc gacccgaagc gctcctgcgc
     8041 cgccagcgtc gcgcgcgcgt tcgccaccgc cgactcgatc ttcagccggt tgcgcacgat
     8101 gcccgcgtcg gcgagcaggc gcgcgaggtc cgcctcgcca tagcgcgcga cacgctcggc
     8161 gtcgaagccg tcgaaggcgc ggcggtagtg ttcgcgcttg cgcaggatgg tgatccagct
     8221 cagccccgcc tgcgcaccct cgagcacgag catctcgaag aggtggcggt cgtcgtgatt
     8281 gggcacgccc cactcggtat cgtggtaggc gacgtagagc ggatcggtgc cgcaccaggc
     8341 gcagcgctcg gacatggatg gtgctcctcc tgagggagtc gtggattcta gtcccgggcg
     8401 ctgcgctcga ggtttagatg aagttcggcg actaatgaag ataccgtcgg agagtccttt
     8461 tgaagccatg tgctgttcgg ccaaagccct gttccatggg atcttgccag ccctgctgaa
     8521 ccggagcagg ccggtactcc cgaactagat attcagggca caatgcgttg tatcaccaac
     8581 gcatgttggt cggacatatc gaggctggat gaaacaaatc cgatgcgcca taacctgagt
     8641 gaagccttcg catttggcaa gctggaccgt tccgaggacg ctatcaaatg gcatcctctg
     8701 gtcgatcatt tagcggacgt cgcggccgtt tttgaggcgc tgtgcgcatg ccgagccatc
     8761 cgccgatcca tcgaggcagt tgctgggcgt agcctcgacc atcgtgactt cgcgcggctt
     8821 gctgccctcg cctttcttca tgaccttggc aaagcgaacg cggggttcca ggcaaagcgc
     8881 tggctaaacg aggaacggcc gcgcggcagg ctgaccgccg ggcacagcgc ggaggcaatc
     8941 gcgctgctcg aagcaagtaa tcacagcgac gaagccgagg cgctgctact gcacctgcct
     9001 attcttgaaa tctgcggctg ggggaagcct gagaccctgg acaacttgct acgcgccagc
     9061 attgcgcacc atggacggcc gatcgccaac gcacccgact ggttcaacgc caggatccat
     9121 tggtccaggc aaggtgagta tgaccccgcc gaacaactaa agagcattgg cgccgccctc
     9181 caacgcttcg ttccagaggc attcgtcgaa gggggcctgc cacttcccga tacgccgcgc
     9241 ttcgcgcatt acttcgccgg cctcgtccaa ttggccgact ggttgggttc ggacactcga
     9301 tttttcccct tcacgaatgt gggcgatcga gatcgcatcg gcaatagccg tgaactggca
     9361 tcgacagcgg tgcgcgagat cggcctcgat cctgagcctt gccgtaagcg gttcgagcag
     9421 gcatcaccag atttcgacga cgtattcgat acgaccactc cctatcccgc ccagtccgca
     9481 atgtccgacc agacgctcgg tcaggttgtc gtgctcgaag ccgagaccgg cagcggcaag
     9541 accgaagccg cgctgtggcg ctaccttcat ctgttcgcgc gcggcgaagt cgacggcctc
     9601 tacttcgccc taccgacccg cgtggccgct tcacaggcct accgccgcgt gcgcgaggcg
     9661 ctttcacgcg cttggcccga cggcgccccg ctcgcagttc gagccttggc cggttatgcc
     9721 gccgcggatg gggaaaccgc tcgggccctg cccgatttca aggtgttgtg gagcgacgat
     9781 ccgtcggatg ccgaagcggg tcggcgatgg gcgggcgaga gctcgaagcg cttcctcgct
     9841 gcaccggttg ccgtcggcac ggtcgaccag gcactgctcg gcacgctgca ggtcaagcat
     9901 gctcacctgc gccatgcgct gacagcgcgt tcgctgcttg tgatcgacga agtgcacgca
     9961 tcggacgcct acatgggcgt gttgatcgaa caactcatcg acgcacaaat tgcgctcggc
    10021 ggccacacgc tgctgttgtc cgcgacactc ggcgcagcgg cccggactcg ctacctcgct
    10081 gccggcaatc gtagggcgca gcgtctgccc tcactcgctc aagcgagctg tctgccctac
    10141 cctgcgataa gcgatgccgc gggccttcat gccacaagcg gcgccccgcg agcaaagcag
    10201 gtcgcttggc agtgtgagga cgccatcgat gacccgcagc gcgtcgccgc acttgcactc
    10261 gaagccgccg aacagggcgc ccgcgtgctg gtgatccgaa acaccgtgcc ggctgccgtc
    10321 gccacccttg cagcgctcga agcggccaca cccaacgcag cctggctctt caattgtgac
    10381 ggcgtcatca cgttgcatca cagccgcttc tcgcgcgaag atcggcccgt gctcgacgcg
    10441 acggtcgaag cccaactcgg aaaggggcgc cccagcggac cgctgatcgt ggtcggcact
    10501 cagacgctgg agcaaagcct ggacctcgac gccgacttcc tgatcaccga cctgtgcccg
    10561 atggacgtgc tgttgcagcg catcggccgc ttgcatcgcc atatgcgccc cgtcgaggct
    10621 cgacccaacg cgttcaggga ggcgcgtgtg cttgtgctga cgcccaacgc aggtaacctc
    10681 gctccatgcc tcacccgccc gcggcacggg ctcgggcgct tccgcgatgg gggcggcgtt
    10741 tatgccgacg tgcgcatcct tgaagctacg cgcaagctga ttcacgccga atctcaggtc
    10801 cgcatcccgg ccgacaaccg gcgcttggtc gagttcgcca cgcacccgga ggcgctgcac
    10861 gccatcgaat cgatgggcac agcttgggcg acgcatggtc agcaggttga cggcgatact
    10921 ggcgcccgcc gcactagcgc caagctagga ctacttcccc tcgacactgc gttcgtcgaa
    10981 ctcggctttc ccgaccaggt gaaactcgca acacgcctcg gcgctgccga ccgcttactc
    11041 actttcgacc cgccgctgca agggccattt gggcaggctg tgcgggagct tccgattagg
    11101 caccacttgc tgccgcgcgc ccttccgctt gaagcgttac cggaggatat cgtcgtcgag
    11161 cacggcggct ttcggtttcg cctcgccgat acgcactatc gctacagccg catcggcctc
    11221 gaaaaaattc cgcaaactgc cgcttcggag taatgcatgg atcgccccca tctgaacctg
    11281 ctcgacgagc ctctgattcg cgttcgcaat ctaggcgggc aaacgcagcg actcacatta
    11341 ccgcaactcc tggctgccct cggtcgtgac gcggtgcgcg actaccctgc actgcgtccc
    11401 caccagcgcc acccctggca cgcgctgctg gtgcagctgg ccgcgcttgc gctgcacgcc
    11461 gccgaccagg aacgtccgtg ggatgaggct accgattggc gcgccgcact gctcgcgctg
    11521 acgcccgatc acccggatgg ttgcgccttt tgcctcgttg cccctcacga ccgtccagct
    11581 ttccttcaac ccccagtgcc aagcggaaag ttggatggct ggaaatacat tgccacccct
    11641 gacgcactcg acatgctggt cacgtcgaag aaccatgatc taaagagcga gcgaatgcgg
    11701 cacgcggagg cggacgactg gctgtttgcc ttggcaagcc tgcaaacgca agaggggttc
    11761 ctcgggaaag ggaactacgg catttctcgc atgaacggag gtttttcgag ccgtccctcc
    11821 ctcggcgtca cacccgcggg cggagtcggt gtgcgatggc gacgcgatat tggcgcactt
    11881 ctcactggac gggacgacat aacggagacc ataggttttc agcgcgatgg cggcatggca
    11941 ctgctctgga tacccccgtg ggacggcaca aagagcctcg cattcacggc gctcgacccg
    12001 ttctacatcg aaatctgtcg gcggatcaga cttgtcctcg actcggatgg ccgcctgaag
    12061 gcgcgcggaa tcggaacctc tgcggctcgc gtagatgccg cggcgcgcaa gggtgtcact
    12121 ggcgacgctt ggacgccggt cgaagacgat aaagcgctta cgcttggttc cgacggtttc
    12181 aactacagcc ttgctgcgga gttagcgtgg ggctccaagt accgtaagac cctcgctcaa
    12241 gcacttcgcg cagaagatgg cactgcagga atttctctgc ttgcacaagg ggtggtacga
    12301 ggccagggaa agacggaggg ttaccacgag cgccggattc ctctcacgaa gaaagccatc
    12361 ggctacctgc gtggcggaca gactgacctg ccggcagcaa tcgccgctgc acgagtgaaa
    12421 gccgtggcga cgatgcgggg atctattttg cgcaccgcac tgttctacct gctcgaagcc
    12481 ggcactgaga agatcgattt cgaccgtcgc actgccaaac aacagatcga agccttcata
    12541 cgtacatttg aacgcaccga ggatgcgcgc ttttttgagg acctgaatcg agagattgaa
    12601 gctgaggaca ggaacgccga gcggctcgcg tggatgctcg ggctcgctga acgcgccgaa
    12661 gtcgtgctga gaggcgcttt cacgatcggc ccccaaagcg gcgagcgtcg atatcgtgcg
    12721 cggtcgcggg cgctgtccta ttttcatggc gcgctacgca gtggcaaaca ccctgaactc
    12781 gtcgcactcg cacagcactt tcgctcttac caagcggcag aggaggctgc gtcatgaacc
    12841 aaaccgacac gacaccgccc gcatcaccgc tgggtcggct tgcgcaagtc atcacgcggc
    12901 ggcagcactt ccgcggcgac gccagcgcac tcgacacggg cgagcaagcc gcgctcgcac
    12961 gtctcgaccc cgaggctccg cggcctcatc aggtcggtgc gctcgcccat gcgctggtcc
    13021 tcgctggaat caaaccggat gactggcagc acacaacctg gcgacgctgg gccttgctgg
    13081 cccaaggcat ggcgctggca ggtcacgacg gctccgcccg actcggcacc caactcgctc
    13141 acgctgcagt gtccgaaagc cgggtcaccc gcctgctcac tgcacgcggt gacgctttca
    13201 gccagcaggt gccgcgtgtg gtgcgtttga tggcgagccg aggcgtttca cccaactggc
    13261 ttgaattcgg tgcgctgatt ctcgccaccg accacgacga agcccgtgcg gagaccctac
    13321 gcttgcgtat cgccggcaat tactactcga ctacccattc ctgaaccgaa tccacccgat
    13381 cgaacaccga caggacatcg ccatgagcct cccgcgcttc atccagatcc acaccctgca
    13441 cacctacccc gctgccctgc tcaaccgtga cgatgccggg ctcgcaaaac gcctccccta
    13501 cggcggcgcg atccgaacgc gtatttcctc acagtgcctc aagcgccact ggcgcgtcgc
    13561 cgacgacgcg ttttcgctcg cgaagctggg cgtaccgatg gcgacgcgca cacgctatgt
    13621 cgccgaattg attcgccaac gccttatcga gcaaggcatc gaagaagcac gcgccttcgc
    13681 taccgccgag gccctgcttg aagccctgtt cggcgagaag gccgacaaga agaaggaagg
    13741 ggccaaggcc ctgcaaaccg ggcaggcggt gctcttcggc aacgaagaaa tcgcttac
//
LOCUS       DAOQNN010000037        13692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1880_length_13692_cov_10.573880, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000037 DAOQNN010000000
VERSION     DAOQNN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 13692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13692
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1880_length_13692_cov_10.573880"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PKV97_04050"
     CDS             complement(<1..408)
                     /locus_tag="PKV97_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB05080.1"
                     /translation="MSEPQLKALASRDNAFVKRLHALAHSARDRRKLDETVLDGAHLV
                     ESALAAGVALRALVVSESGLLREEHRRLLAAAPAEVPRYRLGDALFAHVSPVDSPAGL
                     LATIAMPASTPSAPGDASLIVVDGVQDPGNLGSL"
     gene            complement(401..1012)
                     /gene="rnhB"
                     /locus_tag="PKV97_04055"
     CDS             complement(401..1012)
                     /gene="rnhB"
                     /locus_tag="PKV97_04055"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNB05081.1"
                     /translation="MNAELFDPGLPSSSLICGVDEAGRGPLCGPVVAAAVILDPARPI
                     EGLADSKKLSEAARERLAPLIRARALAWAVAEASVEEIDRLNILHATMLAMQRAVMAL
                     GIAPAEVRIDGNRCPKLPYPARAIVKGDATEPSISAASILAKTARDAAMRELDAQWPQ
                     YGLAAHKGYPTAAHLAALAAHGVQAFHRRSFAPVKKLLERRDE"
     gene            complement(1009..2166)
                     /gene="lpxB"
                     /locus_tag="PKV97_04060"
     CDS             complement(1009..2166)
                     /gene="lpxB"
                     /locus_tag="PKV97_04060"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNB05082.1"
                     /translation="MKIRIAMVAGETSGDLLASHLIRALRRHLPDAEFFGIGGPKMQA
                     EGFDVRWPCELLAVHGYVDALKRYRELSGIRRALLAQIRAERPDAFIGVDAPDFNLWL
                     EGKVRDAGIPAIHFVGPSIWAWRGGRIKRIARSVSHMLCLFPFEPELYERAGVPVSYV
                     GHPLADEFPLEPDRAAARERLGIPLERGVVAMLPGSRQSEVRNLADIFIGTAKILHER
                     DPERLFLVPLATRETRQLFEEALHRQDASGLPIRMLFGHAVEAMTAADVVLVASGTAS
                     LEAALLKRPMVITYRIGKWQYRLMKRMAYLPWVGLPNILCGETVVPELLQDEADPEHL
                     AAAIDDWFADDARRAAVAARFSDLHRTLRQDTARRAAEAILPYLDKAGPRA"
     gene            complement(2207..2977)
                     /gene="lpxA"
                     /locus_tag="PKV97_04065"
     CDS             complement(2207..2977)
                     /gene="lpxA"
                     /locus_tag="PKV97_04065"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNB05083.1"
                     /translation="MIHPTAIVHPGAKLGANVSVGAYSLIGENVEIGDGTRIGPHVVV
                     EGHTRIGRDNEIFQFCSIGASPQDKKYDAEPTRLEIGDRNTIREFCSFNVGTSQDAHV
                     TRVGNDNWIMAYVHIAHDCQVGDHTIFANNATLAGHVHVGDWAILGGFTGVHQFCRVG
                     AHSFCGVGTVLLQDLPPFVTVAGNPAKPHGINSEGLKRRGYSAEGIAAIKRAYRALYR
                     SGLTLDEARQRVAEIAAGQAEAAPFAAFIADSGRGIVR"
     gene            complement(2974..3408)
                     /gene="fabZ"
                     /locus_tag="PKV97_04070"
     CDS             complement(2974..3408)
                     /gene="fabZ"
                     /locus_tag="PKV97_04070"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNB05084.1"
                     /translation="MDIKEILQYLPHRYPFLLVDRMLEIEPGVRARGLKNVTMNEPFF
                     PGHFPHHPVMPGVLIIEAMAQAAALLSFKSTGVKPDDNSVVYFAGIDNARFKRPVVPG
                     DQLIFDVEITHAKRNIYKYKARASVDGELAAEAELMCAIKTL"
     gene            complement(3415..4449)
                     /gene="lpxD"
                     /locus_tag="PKV97_04075"
     CDS             complement(3415..4449)
                     /gene="lpxD"
                     /locus_tag="PKV97_04075"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNB05085.1"
                     /translation="MFRLHELVDRLGGEIAGDGDVAVSRVGTLDKAGEGDLAFLANRK
                     YLSQVGECAASALIVTPDLREALAGRNLILTADPYLYFARVAQLFNPPPAVRPGIHAL
                     AAVASTLPASVQVGPGASIEEDVVLGEDVVIGPNCHVGRGTRIGRGTRLYANVSIYHD
                     CVIGEDCILHSGVVIGADGFGFAREKSGAWVKIPQTGRVVLGNDVEIGANTTVDRGAL
                     DDTVIGDGVKLDNLIQIAHNVRVGEHTIMAGCAGVAGSARIGARCMIGGQAGISGHLS
                     IADDVVVSAWTLVAKSIAKPGVYTGNLPLQTHGDWVKNFSHLRHLDALARRVRQLEQN
                     SDGPDDRSQG"
     gene            complement(4463..4963)
                     /locus_tag="PKV97_04080"
     CDS             complement(4463..4963)
                     /locus_tag="PKV97_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNB05086.1"
                     /translation="MKVSTLSLVALAVLGLSQTAFAETKIGFVNSDRVMREAAPAVRA
                     QQRLEKEFEKRDQELQRIARDLKSMQEDLERNGPTMGDSDRRNKERAFNELNRDFQRK
                     QREFREDLNQRRNEELASVLDKANRSVKQIAEQENYDIIFQEAVYANPRIDITDKVIK
                     TLSDAK"
     gene            complement(4998..7352)
                     /gene="bamA"
                     /locus_tag="PKV97_04085"
     CDS             complement(4998..7352)
                     /gene="bamA"
                     /locus_tag="PKV97_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNB05087.1"
                     /translation="MNRKLLPGLLVALFAAAPALAFDPFVVKDIRVEGIQRTEAGTVF
                     NYLPVRVGETFTEAQAADAIRALFATGFFSDVRIETEGNVIVVVVDERAAIADITFDG
                     VKEFDKDALKKGLREVGLAESRIFDRSLLERAEQELKRQYLSRGKYAATVTTTVTPLE
                     RNRVGINFKVDEGGVAKIRQIRIVGARAFEEEDLLDEMQLTTPGWLTWYTKNDQYSRQ
                     KLSADLETLRSWYLNQGYLDFNIDSTQVSITPDKQDIYITVNITEGEKYTVTGVRFTG
                     DLILPEAEYQALTKIRPGETFSRERLTETTKAITDRLGNEGYAFANVNAAPEVDKVKR
                     EVAFTVFVDPGRRVYVRRINVGGNTKTRDEVVRREMRQMEGGWYDAAAINRSRTRIDR
                     LGFFDEVTVETPAIPGTTDQVDVNFTVKERPTGNLMLGAGFSSSENIVLSASISQQNL
                     FGSGNALTLGLNTSQSSRTLALSFTNPYYTVDGISLGWDLYHRTYDADELDSVSPYKT
                     VSTGAGIRLGWPIAEDDHVNFGLTADRTEITTYNASPLLYKDFCKENGLDNPSGVGDC
                     TVDSLLLTVGWSRDGRDSFTYPRSGVYQRVYGEVTVPVGDLRYGKLGYQYQHWFPIGT
                     DYALMLNADVGYAKGFGGKDVPFYKNYYVGGIGSVRGFDQGSVGPKYKDTDGDISSTG
                     GTRKAVANAEFFFPMPGAGKDRSFRISAFLDAGYVWGRDEEPLGGGTFVDQNIRFSDL
                     RYSTGLAFSWSSPIGPLKFSFGHPLKKEENDEIQRFQFQLGSVF"
     gene            complement(7361..8725)
                     /gene="rseP"
                     /locus_tag="PKV97_04090"
     CDS             complement(7361..8725)
                     /gene="rseP"
                     /locus_tag="PKV97_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNB05088.1"
                     /translation="MTLLDYLVPFALALGLLILVHELGHYLVARWCGVKVLRFSIGFG
                     KPLLRHTVGSDGTEWVLAAFPLGGYVKMLDEREAPVAAPELHRAFNRQSVYRRFAIVA
                     AGPLANFLLAIALYWGLFVGGTEELKPRVALSDTPAIAQAAGVREGDLVIAVDEEPVR
                     SWQDLRWVLLRHALDNREVVLRVRTLEDVDAFRRMDLSGVAIDEGNTDLIARLGLRPW
                     RPALPAVIGRIADGSAAERAGLQVGDRVLAISGTAVAAWADLVRLVREAPGRALDFEI
                     DRAGGVVGLVVTPDAAEEGGARIGRIGVGVGEAATGGIEMFGEIRYGVLEGLARAVRQ
                     TWETSVLSLKMIGRMLTGEVSWKNLSGPVTIADYAGQTAQLGLAHYLKFVALISISLG
                     VLNLLPIPVLDGGHLLYYTVEIIKGGPIPERIMEVGQQIGLALLVMLMAFAFYNDLNR
                     LISG"
     gene            complement(8722..9924)
                     /gene="ispC"
                     /locus_tag="PKV97_04095"
     CDS             complement(8722..9924)
                     /gene="ispC"
                     /locus_tag="PKV97_04095"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNB05089.1"
                     /translation="MPDPAPQRLTVLGATGSIGQSTLDVVARHPDRFEVFALSAQRQD
                     DKLLEQCLRFSPRFAVMGDATAAERLRAGLRAAGRDTEVLCGEEALEHVAAADEVAMV
                     MAAIVGAAGLRPTLAAARAGKKVLLANKEALVLSGRLLIDAVAASGAVLLPIDSEHNA
                     IFQALPAGYAREPARAGVRRVLLTASGGPFRTAPIDSLANVTPEQACAHPNWVMGRKI
                     SVDSATMMNKGLEVIEAHWLFGAPADMIEVVVHPQSVIHSMVDYADGSVIAQLGNPDM
                     RTPIANAMAWPERIDAGVRPLDLFEIARLDFERPDFARFPCLKLAFDALRAGGTAPAV
                     LNAANEEAVAAFLDHRIGFLDIAAVIAASLERSRPGAVDSLEAVLAADAHARELARDE
                     ISRLRRDR"
     gene            complement(9970..10791)
                     /locus_tag="PKV97_04100"
     CDS             complement(9970..10791)
                     /locus_tag="PKV97_04100"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNB05090.1"
                     /translation="MLKDRVLTAIALLVGLLAAVFLLPASVWLVLCAVLCGAAAWEWG
                     ALARFERGLRVSTAVAFAAASAFLGWSAGLGSGELGGAAAVLPLYVFSALFWIFVVPM
                     WLRGKWALSNPMLASLVGFVVLVPTALAMMQLRTMGPGVLLGTMAVVWVADIAAYFSG
                     RAFGRHKLAPAISPGKTWEGAAGGVLAVLAFGFGVLAMRGAEFEGGAPVIAGVLLAWT
                     AVSIVGDLFESLLKRQAGLKDSGNILPGHGGILDRIDSLTSTLPLVALGAVWLAR"
     gene            complement(10799..11566)
                     /gene="uppS"
                     /locus_tag="PKV97_04105"
     CDS             complement(10799..11566)
                     /gene="uppS"
                     /locus_tag="PKV97_04105"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNB05091.1"
                     /translation="MIGGLFRSSTRDIPDVSQVPKHIAVIMDGNGRWARKRMLPRVAG
                     HARGVEALRATVRGCVDRGVEYLTVFAFSSENWRRPQDEVSFLMQLFLKSLQKEIRRM
                     HDNDMRFRVIGDLSKFDPRLVDMIREAETLTAGNGGLNFTVAANYGGRWDILDAMSRL
                     MAREPGRLERFTEDEIASELSMSFAPEPDLFIRTGGEKRISNFLLWQLAYTELYFTDV
                     LWPDFDDGALGQAIGSYQGRERRFGRTSEQLKADAGE"
     gene            complement(11619..12176)
                     /gene="frr"
                     /locus_tag="PKV97_04110"
     CDS             complement(11619..12176)
                     /gene="frr"
                     /locus_tag="PKV97_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNB05092.1"
                     /translation="MISELKKNIEQKMLKSVEALKTDLAKVRTGRAHTGLLDHVMVEY
                     YGSMVPVNQVANITLIDARTIGVQTWEKPMLGKVERAIRDSDLGLNPANMGELLRVPM
                     PALTEERRRDLTKVVRHEGETAKVAIRNLRRDGNQQLKDAVKDKAISEDDERRGEDEI
                     QKLTDRYVAEIDKLLAQKEQELMQI"
     gene            complement(12195..12908)
                     /gene="pyrH"
                     /locus_tag="PKV97_04115"
     CDS             complement(12195..12908)
                     /gene="pyrH"
                     /locus_tag="PKV97_04115"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNB05093.1"
                     /translation="MSAAYKRILLKLSGEALMGDDAYGINEEVVSRIVSEVAEISRLG
                     VQVGVVIGGGNIFRGMKGAASGMDRATADYMGMLATVMNAMALADAMRRMGLEARVQS
                     ALRIDQVVEPYIRGRAIRHLEEGRVVIFAAGTGNPFFTTDTAAALRGAEIGAQIVLKA
                     TKVDGVYTADPKKDPQGQRYHRISFDEAIGRNLQVLDSTAFALCRDQKLPINVFSIFK
                     PGALKRVVMGEDEGTLVHS"
     gene            complement(12919..>13692)
                     /gene="tsf"
                     /locus_tag="PKV97_04120"
     CDS             complement(12919..>13692)
                     /gene="tsf"
                     /locus_tag="PKV97_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNB05094.1"
                     /translation="RVKLGNKASKAAARIAAEGVVGIDISADGKLGSIIEVNCETDFV
                     AKNDDFLALVQNCAGLVAAKSPADVAALSALPLGEGTVESTRTALVGKIGENMSIRRF
                     ARIEAKGKLFSYIHGGAKIGVLLDVVGGDEQLGKDIAMHIAASKPKALDASGVSQDLI
                     DAERRIAIEKARADNKPEAMLEKIADGTVQKFLKEVTLLAQVFVKAEDGKQTIEQLLK
                     AKGASVAGFTLFIVGEGIEKKVTDFAAEVAEQAAAAAKK"
BASE COUNT         2158 a         4513 c         4743 g         2278 t
ORIGIN      
        1 caggctgccg aggttgccgg ggtcctgcac gccgtcgacc acgatcaggc tggcgtcgcc
       61 cggggcgctc ggcgtgctcg ccggcatggc gatggtggcc agcaagccgg cggggctgtc
      121 gaccgggctg acatgcgcga acaaggcatc gcccaggcgg tagcgcggca cctctgccgg
      181 cgccgccgcg agcaggcgac gatgctcctc gcgcagcagg ccggactccg acaccaccag
      241 cgcgcgcaag gccacgcccg ccgcgagtgc ggattcgacc aggtgggcgc cgtcgagcac
      301 ggtctcgtcg agcttgcggc ggtcgcgtgc cgagtgcgcg agcgcatgca ggcgcttgac
      361 gaaggcgttg tcacgcgatg ccagcgcctt gagctgcggc tcactcatcc cggcgctcga
      421 gcagcttctt caccggcgcg aagctgcgcc ggtggaaggc ctgcacgccg tgcgccgcga
      481 gcgccgcaag gtgggcggcg gtcgggtagc ccttgtgagc ggccagcccg tactgcggcc
      541 actgcgcgtc gagctcgcgc atggctgcat cgcgtgcggt cttggcgagg atggaggccg
      601 ccgagatcga tggctcggtg gcgtcgccct tgacgatcgc ccgcgccgga tagggcagct
      661 tggggcagcg gttgccgtcg atgcgcacct cggccggcgc gatcccgagc gccatcaccg
      721 cgcgctgcat ggccagcatg gtggcgtgca ggatgttgag gcggtcgatc tcctcgacgc
      781 tcgcctcggc caccgcccag gccagcgcac gcgcgcggat cagcggcgcg aggcgctcgc
      841 gcgcggcttc gctcagcttc ttggaatcgg cgaggccttc gatcgggcgc gcgggatcga
      901 ggatgaccgc agcggcgacc actggcccgc acagcggccc gcggccagcc tcgtcgaccc
      961 cgcagatcag gctggaggac ggcagtccgg gatcgaacag ctcggcgttc atgcccttgg
     1021 tcccgccttg tcgaggtagg gcaggatcgc ctccgcggcg cgccgcgcgg tgtcctgccg
     1081 gagggtgcga tgcaggtcgg agaagcgcgc cgcgaccgca gcgcgccgcg catcgtcggc
     1141 gaaccagtcg tcgatcgcgg cggcaaggtg ttcggggtcg gcctcgtcct ggagaagctc
     1201 cggcaccacg gtctcgccgc acaggatgtt gggcaggccc acccagggca ggtaggccat
     1261 ccgcttcatg aggcggtact gccacttgcc gatgcggtag gtgatcacca tcgggcgctt
     1321 gagcagcgcc gcctccaggc ttgccgttcc gctcgccacc agcaccacgt cggctgcggt
     1381 catcgcctcg acggcgtgac cgaacagcat gcggatcggc agcccgcttg catcctgtcg
     1441 gtgcagcgcc tcctcgaaga gctggcgcgt ctcgcgcgtg gccagcggga ccaggaagag
     1501 gcgctcggga tcgcgctcgt gcaggatctt cgcggtgccg atgaagatgt cggcgagatt
     1561 gcgcacttcg gactgccggc tccccggcag catcgccacc acgccgcgct cgagaggaat
     1621 gccgaggcgt tcgcgcgcag cggcgcggtc gggctcgagc ggaaattcgt cggcgagcgg
     1681 gtggccgaca tagctcaccg gcacgccggc acgctcgtag agctcgggct cgaaggggaa
     1741 gaggcacagc atgtgcgaca ccgagcgcgc gatgcgcttg atccgcccac cccgccaggc
     1801 ccagatcgac gggccgacga aatggatcgc cgggatgccg gcgtcgcgca ccttgccttc
     1861 gagccacagg ttgaaatcgg gcgcatccac gccgatgaag gcatcggggc gctcggcgcg
     1921 gatctgcgcg agcagcgcgc ggcggatgcc ggagagctcg cggtagcgct tgagcgcgtc
     1981 gacgtagcca tgcaccgcga gcagctcgca gggccagcgc acgtcgaagc cttcggcctg
     2041 catcttcggc ccgccgatgc cgaagaactc cgcgtcgggc agatggcggc ggagcgctcg
     2101 gatcaggtgg ctcgcgagca ggtcgccgga ggtctccccg gcgaccatgg cgatgcggat
     2161 cttcatgccg ttcgagacgc ggcccgcgcc gtgcagcgga tcatgctcag cgcacgatgc
     2221 cgcgaccgga gtccgcgatg aaggcggcga agggcgcggc ctcggcctgc ccggcggcga
     2281 tctcggcgac gcgctggcgc gcctcgtcga gcgtgaggcc gctgcggtag agcgcgcggt
     2341 aggcgcgttt gatcgcggcg atgccctcgg cactgtagcc gcggcgcttg aggccttcgc
     2401 tgttgatgcc gtgcggtttg gccgggttgc ccgccacggt cacgaagggc ggcagatcct
     2461 gcagcagcac ggtaccgaca ccgcagaagc tgtgcgcgcc gacgcggcag aactgatgca
     2521 ccccggtgaa gccgccgagg atcgcccagt cgcccacatg gacatggccg gcgagcgtgg
     2581 cgttgttggc gaagatggtg tggtcgccga cctggcagtc gtgcgcgatg tgcacgtagg
     2641 ccatgatcca gttgtcgttg ccgacgcggg tgacgtgcgc gtcctggctg gtgccgacgt
     2701 tgaacgagca aaattcgcgg atcgtgttgc ggtcgccgat ctcgagccgg gtgggctcgg
     2761 cgtcgtactt cttgtcttgc gggctggcac cgatcgagca gaactggaag atctcgttgt
     2821 cgcggccgat gcgggtgtgg ccctcgacca cgacgtgcgg gccgatgcgc gtgccgtcgc
     2881 cgatctcgac gttctcgccg atcagcgaat aggccccgac gctgacgttg gcgccgagct
     2941 tcgcacccgg atggacgatg gcggtcgggt ggatcatagc gtcttgatcg cgcacatcag
     3001 ctcggcttcg gcggcgagct cgccgtcgac gctggcacgt gccttgtact tgtagatgtt
     3061 gcgcttggcg tgggtgatct cgacgtcgaa gatcagttgg tcgcccggca ccaccggacg
     3121 cttgaagcgt gcgttgtcga tgcccgcgaa gtacaccacc gagttgtcgt cgggcttcac
     3181 gccggtgctc ttgaacgaca gcagcgccgc agcctgggcc atggcctcga tgatcagcac
     3241 gcccggcatc accgggtggt gcgggaagtg ccccggaaag aagggctcgt tcatggtcac
     3301 gttcttcagc ccgcgcgcac gcacgcccgg ttcgatctcc agcatgcgat cgacgagcag
     3361 gaagggatag cggtgcggca ggtactgcag gatttccttg atgtccatga tggctcagcc
     3421 ctgcgagcgg tcgtcgggac cgtcgctgtt ttgttcgagt tggcggacgc gcctggcgag
     3481 cgcgtcgagg tggcgcaggt gggagaagtt cttcacccag tcgccatgcg tctgcagcgg
     3541 caggttgccg gtgtagacgc ccggtttggc gatcgacttt gcgaccaggg tccacgccga
     3601 caccacgacg tcatcggcga tgctcaggtg tccggagatg ccggcctggc cgccgatcat
     3661 gcagcgcgcg ccgatccggg cgctgcccgc gaccccggcg cagccggcca tgatcgtgtg
     3721 ctcgcccacg cgcacgttgt gcgcgatctg gatgaggttg tcgagcttga cgccatcgcc
     3781 gatcacggtg tcgtcgagcg caccgcggtc caccgtcgtg ttggcgccga tctcgacgtc
     3841 gttgccgagg accacacggc cggtctgcgg gatcttgacc caggcgccgg acttctcgcg
     3901 cgcgaagccg aagccgtcgg cgccgatgac cacgcccgaa tgcaggatgc agtcctcgcc
     3961 gatcacgcag tcgtggtaga tgctgacgtt ggcatagagc cgggtgccgc gcccgatgcg
     4021 agtgccgcgc cccacgtggc agttgggccc gatcaccacg tcctcgccga gcaccacgtc
     4081 ttcctcgatc gaggcgccgg gtcccacctg cacgctggcc ggcagggtgc tggcgaccgc
     4141 ggcgagcgca tggataccgg gacgcaccgc ggggggcgga ttgaacagct gcgcgacgcg
     4201 tgcgaagtac aggtaggggt cggcggtcag gatcaggttg cgcccggcca gcgcctcgcg
     4261 caggtcgggc gtgacgatca gcgcacttgc cgcacactcg ccgacctgcg acaggtactt
     4321 gcggttggca aggaaggcga gatcgccctc ccccgccttg tccagcgtgc cgacgcgcga
     4381 aaccgcgaca tcgccgtctc ccgcgatctc gccgcccagg cgatcgacca gctcgtgcag
     4441 tcggaacatc gggcgaccag tcttacttgg cgtcggacag ggtcttgatg accttgtcgg
     4501 tgatgtcgat gcgcgggttc gcgtacaccg cttcctggaa gatgatgtcg tagttttcct
     4561 gctcggcgat ctgcttgacc gagcggttgg ccttgtcgag caccgaggcg agttcctcgt
     4621 tacggcgttg gttcaggtct tcgcggaact cgcgctgctt gcgctggaag tcgcggttga
     4681 gctcgttgaa cgcacgttcc ttgttgcgac ggtcgctgtc gcccatggtc gggccgttgc
     4741 gctcgaggtc ttcctgcatc gacttgaggt cgcgcgcgat gcgctgcagc tcctggtcgc
     4801 gcttttcgaa ctccttctcc agccgctgct gcgcccgcac ggcgggcgct gcttcgcgca
     4861 tgacgcgatc ggaattgacg aagccgatct tggtttcggc gaacgcggtc tgggaaaggc
     4921 cgagcacagc gagtgcgacc agggagaggg tactgacttt caattatgcc tccggatgaa
     4981 actgtttcgc ccgcagctca gaacacgctg ccgagctgga actggaaccg ctggatctcg
     5041 tcgttctctt ccttcttgag cggatggccg aagctgaact tcaacgggcc gatcggcgag
     5101 ctccacgaga atgccaagcc ggtactgtag cgcagatcgc tgaaacggat gttctgatcc
     5161 acgaaagtgc cgccgccgag cggttcttcg tccctgcccc agacgtagcc ggcatcgagg
     5221 aaggccgaga tgcggaagga acggtccttg cccgcgcccg gcatcgggaa gaagaactcg
     5281 gcgttggcga cggccttgcg ggtgccgccg gtcgagctga tgtcgccgtc ggtatccttg
     5341 tacttggggc cgaccgagcc ttggtcgaag ccgcgtaccg agccgatgcc gccgacgtag
     5401 tagttcttgt agaagggtac gtccttgccg ccgaagccct tggcgtagcc gacgtccgcg
     5461 ttgagcatca gggcgtagtc ggttccgatc gggaaccagt gctggtactg gtagccgagt
     5521 ttgccgtaac gcagatcgcc aaccgggacg gtgacctcgc catagacacg ctgatacacg
     5581 ccgctgcggg gataggtgaa gctgtcgcga ccgtcgcgcg accagccgac ggtcaacagc
     5641 aggctgtcaa ccgtgcagtc gccgacgccg ctggggttgt cgaggccgtt ttccttgcag
     5701 aaatccttgt agagcagcgg actcgcgttg tacgtggtga tctcggtgcg gtcggcggtg
     5761 agcccgaagt taacgtggtc gtcttccgcg atcggccagc cgagccgaat gccggcgccg
     5821 gtcgagacgg tcttgtacgg cgacaccgag tcgagttcgt cggcgtcgta ggtgcggtgg
     5881 tacaggtccc aacccaggct gatgccgtcc accgtgtagt aggggttggt gaaggacagc
     5941 gcgagcgtgc ggctggactg actggtgttg aggccgagcg tcagggcgtt gccgctgccg
     6001 aacaggttct gctgcgagat cgacgccgac agcacgatgt tttcggagct ggagaaaccg
     6061 gcgccgagca tcaggtttcc ggtcgggcgt tccttgacgg tgaagttcac gtcgacctgg
     6121 tcggtcgtgc ccggtatcgc cggcgtctcg acggtcacct cgtcgaagaa gccgaggcgg
     6181 tcgatgcgcg tacgcgagcg gttgatcgcc gccgcgtcgt accagccccc ttccatctgg
     6241 cgcatctcgc ggcgcaccac ctcatcgcgc gtcttggtgt tgccgccgac gttgatgcgg
     6301 cgcacataaa cccgccgacc cggatcgacg aatacggtga aggccacctc gcgcttgacc
     6361 ttgtcgactt ccggcgcggc attgacgttg gcgaaggcat aaccctcgtt gccaaggcgg
     6421 tcggtgatcg ccttggtggt ctccgtcagg cgttcgcgcg agaaggtttc gcccggtcgg
     6481 atcttcgtca gcgcctggta ttcggcctcg ggaaggatca ggtcgccggt gaagcgcacg
     6541 ccggtgacgg tgtacttctc gccctcggtg atgttcaccg tgatgtagat gtcctgcttg
     6601 tccggtgtga tcgagacctg ggtggagtcg atgttgaagt cgaggtagcc ctggttcagg
     6661 taccaggagc gcaaggtctc caggtcggcc gagagcttct ggcgggagta ctggtcgttc
     6721 ttggtgtacc aggtcaacca gccgggcgtg gtgagctgca tctcgtcgag gagatcttct
     6781 tcttcgaaag ccctcgcgcc gacgatgcgg atctggcgga tcttggccac gcccccctcg
     6841 tccaccttga agttgatgcc gacacggttg cgttcgagcg gcgtgacggt cgtcgtgacc
     6901 gtggccgcgt acttgccgcg cgagaggtac tggcgcttga gttcctgctc cgcgcgctcg
     6961 agcaacgagc gatcgaagat gcgcgattcg gcgaggccga cctcgcgcaa gcccttcttg
     7021 agcgcgtcct tgtcgaactc cttgaccccg tcgaaggtga tgtcggcgat cgctgcgcgc
     7081 tcgtccacca ccaccacgat gacgttgcct tcggtctcga tgcgaacgtc gctgaagaaa
     7141 cccgtggcga acagcgcgcg gatcgcatcg gcggcctgcg cctcggtgaa ggtctcgccg
     7201 acgcggaccg gcaggtagtt gaacacggtg cccgcctcgg tgcgctggat gccctcgacg
     7261 cggatgtcct tgaccacgaa gggatcgaag gcgagggccg gcgctgcggc aaaaagggcc
     7321 accaggagcc cgggcaggag cttgcgattc atgcgggagt tcagccggaa ataagacgat
     7381 tgagatcgtt gtagaaggcg aatgccatca acatcaccag gagggcgagg ccgatctgct
     7441 gaccgacctc catgatgcgc tccgggatcg gaccgccctt gatgatttcg accgtatagt
     7501 acaacaagtg ccccccatcc agaaccggga tgggcagcag gttcaggacc cccaggctga
     7561 tgctgatcaa ggccacgaac ttgaggtagt gcgccaaacc cagctgggcg gtctgacctg
     7621 catagtcggc gatcgtgacc ggtccggaga ggttcttcca ggacacttcg ccggtgagca
     7681 tgcggccgat catcttgagg ctcagcacgc tggtctccca ggtctgccgg accgcgcgcg
     7741 cgaggccttc gagcacaccg tagcggatct cgccgaacat ctcgatgccg cccgtcgcgg
     7801 cttcgcccac ccccacgccg atgcggccga tccgcgcccc gccctcctcg gccgcgtccg
     7861 gggtcaccac aagccctacc accccgcccg cacggtcgat ctcgaagtcg agcgcgcgtc
     7921 cgggcgcctc gcgcaccagg cgcacgaggt cggcccaggc cgcgaccgcc gtgcccgaga
     7981 tcgccagcac gcgatccccg acctgcagtc ccgcacgttc ggcagcgctg ccatccgcga
     8041 tcctgccgat caccgccgga agcgccggac gccaggggcg caggccgagg cgcgcgatca
     8101 ggtcggtgtt gccctcgtcg atcgccacgc ccgagagatc catgcggcgg aaggcatcga
     8161 cgtcctccag ggtgcgtacg cgcagcacca cctcgcggtt gtcgagcgcg tggcgcagca
     8221 gcacccagcg caggtcctgc cagctgcgca cgggctcctc gtccaccgcg atcacgaggt
     8281 cgccctcgcg cacccctgcc gcctgcgcga tcgccggcgt gtcggacaac gccacgcgag
     8341 gcttgagttc ctcggtgccg ccgacaaaga ggccccagta gagggcgatc gcgagcagga
     8401 agttggcgag cggcccggcg gcgacgatcg cgaaacgccg atacacgctc tggcggttga
     8461 aggcgcggtg cagctcgggt gccgctacag gcgcctcgcg ctcgtcgagc atcttgacgt
     8521 agccgccgag cgggaacgcg gcgagcaccc attcggtgcc gtcgctgccg acggtatggc
     8581 gcagcagcgg cttgccgaag ccgatcgaga agcgcagcac cttgaccccg caccaacgtg
     8641 ccacgaggta gtggccgagt tcgtggacga ggatgagcag gccgagagca agcgcgaagg
     8701 gaaccaggta atctaagaga gtcatcggtc gcggcgaagt cgggagatct cgtcccgcgc
     8761 gagttcgcgg gcgtgcgcgt cggcggccaa taccgcctcc agcgaatcga ccgcgcccgg
     8821 ccggctgcgt tccaggctcg ctgcgatcac ggcggcgatg tcgaggaagc cgatgcgatg
     8881 gtcgaggaag gcggcgaccg cctcctcgtt ggcggcgttg agcacggcgg gcgccgtgcc
     8941 gccggcgcgc agcgcgtcga aggccagctt caggcagggg aagcgggcga agtccggacg
     9001 ttcgaaatcg aggcgggcga tctcgaacag gtcgagcggg cgcacacccg cgtcgatgcg
     9061 ctccggccag gccatggcgt tggcgatcgg cgtgcgcatg tcggggttgc cgagctgtgc
     9121 gatcaccgag ccgtcggcgt agtcgaccat cgagtggatc acgctctgcg gatgcacgac
     9181 cacctcgatc atgtccgccg gggcgccgaa cagccaatgc gcctcgatga cctccaggcc
     9241 cttgttcatc atggtcgcgg aatcgaccga gatcttgcgc cccatgaccc agttgggatg
     9301 ggcgcaggcc tgctcggggg tgacgttcgc gagcgaatcg attggtgccg tgcggaaggg
     9361 gccgccagag gccgtcagca acaccctgcg cacgccggcg cgcgcgggtt cgcgcgcgta
     9421 gccggcgggc agggcctgga agatcgcgtt gtgctcgctg tcgatcggca gcaggaccgc
     9481 cccgcttgcg gccactgcat cgatcagcaa gcggcccgac agcaccaggg cctccttgtt
     9541 ggccagcagc accttcttgc ccgcgcgcgc ggcggccagg gtcgggcgca ggccggcggc
     9601 gcctacgatc gcggccatca ccatggccac ctcgtcggcg gcggcgacat gctcgagcgc
     9661 ctcttcaccg catagcacct cggtgtcgcg acccgctgcg cgcaatcccg cgcgcaggcg
     9721 ttcggcagcg gtcgcatcgc ccatcaccgc gaagcgcggc gaaaaacgca ggcactgctc
     9781 gagcagcttg tcatcctggc gctgcgcact gagcgcgaag acctcgaaac ggtcggggtg
     9841 acgggcgacg acatcgagcg tgctttggcc gatcgagccg gttgcgccca ggacggtcag
     9901 gcgctgcggt gcggggtcag gcatggggag aatccgttgc ggaaggagca cggcggaagc
     9961 cgtggaggat cagcgcgcga gccacaccgc gccgagcgcc acgagcggga gcgtcgacgt
    10021 caggctgtcg atgcggtcga ggatgccgcc gtggccgggc aggatgttgc cactgtcctt
    10081 gaggccggcc tgacgcttga gcagcgactc gaagaggtcg ccgacgatgc tcaccgcggt
    10141 ccacgccagc aggacgccgg cgatcaccgg cgcgccgccc tcgaattccg cgccgcgcat
    10201 cgcgagcacg ccgaagccga aggccagcac cgccagcacg ccacctgcgg cgccctccca
    10261 agtcttgccc gggctgatcg ccggagccag cttgtgacgg ccgaaggcgc ggccgctgaa
    10321 ataggcggcg atgtcggcca cccagacgac ggccatggtt ccgagcagca ccccggggcc
    10381 catcgtgcgc agctgcatca ttgccagcgc ggtcggcacg agcacgacga agccgaccag
    10441 cgaagccagc atcgggttgg acaaggccca cttgccgcgc agccacatcg gcacgacgaa
    10501 gatccagaat aacgcgctga agacatagag cggcaacacg gccgcagcac cgccgagctc
    10561 gccgctgccc agccccgcgc tccaacccag gaacgcggat gcggcggcaa aggcgaccgc
    10621 cgtggacacg cgcaacccgc gctcgaagcg ggcgagcgcg ccccactccc acgccgccgc
    10681 accgcacaag actgcgcaga gcacgagcca gaccgatgcc ggcagcaaga acaccgccgc
    10741 cagcaggccg accagcaagg cgattgcggt gaggacgcga tccttgagca tggaggccct
    10801 attccccggc gtcggccttg agctgctcgc tcgtgcggcc gaagcgccgc tcccgcccct
    10861 gataggagcc gatggcctgc ccgagcgcac cgtcgtcgaa gtcgggccag agcacgtcgg
    10921 tgaagtagag ctcggtatag gcgagctgcc agagcaggaa gttgctgatg cgcttctcgc
    10981 cacccgtgcg gatgaagaga tcgggctccg gcgcgaagct catcgacagc tcggaggcga
    11041 tctcatcttc ggtgaagcgt tccaggcgcc ccggttcgcg cgccatcagg cgcgacatcg
    11101 catcgagaat gtcccagcga ccgccgtagt tggccgcgac ggtgaagttg agtccgccat
    11161 tgccggcggt gagcgtctcg gcctcgcgga tcatgtccac cagccgcgga tcgaacttcg
    11221 agaggtcccc gatcacgcgg aagcgcatgt cgttgtcgtg catgcggcgg atctccttct
    11281 gcagcgactt cagaaagagc tgcatcagga aagaaacctc gtcctgcggg cggcgccagt
    11341 tctcggagct gaacgcgaac acggtgaggt actccacccc gcgatcgacg cagccgcgca
    11401 cggtggcgcg cagcgcctcg acaccgcgcg catgccccgc gacgcgaggc agcatgcgct
    11461 tgcgcgccca gcgcccattg ccgtccatga tgacggcaat gtgcttcggc acctgcgaga
    11521 cgtcgggaat gtcgcgggtg gaactcctga acagccctcc gatcatgctc cgcctcctgt
    11581 cgtgcccgtt gacgatacag ccgatccgcg cacgcggatc agatctgcat gagttcctgc
    11641 tctttctggg cgagcagctt gtcgatctcg gctacatagc ggtcggtgag cttctggatc
    11701 tcgtcctcgc cgcgacgctc gtcgtcctcg gagatcgcct tgtccttgac cgcatccttg
    11761 agctgctggt tgccgtcgcg gcgcaggttg cggatcgcca ccttggcggt ctcgccctcg
    11821 tggcgcacca ccttggtgag gtcgcgacgg cgctcctcgg tgagcgcagg catcggcacg
    11881 cgcagcagtt cgcccatgtt cgcggggttg aggccgaggt cgctgtcgcg gatcgcgcgc
    11941 tcgaccttgc cgagcatcgg cttttcccag gtctgcacgc cgatcgtgcg ggcgtcgatc
    12001 agggtgatgt tggcgacctg gttcaccggc accatcgaac cgtagtactc gaccatcacg
    12061 tgatcgagca ggccggtgtg ggcgcggccg gtgcgcacct tggcgaggtc ggtcttgagc
    12121 gcctcgaccg acttcagcat cttctgctcg atgttcttct tgagttcgga aatcatgcaa
    12181 gccctctccg gaggtcagga atggaccagc gtgccttcgt cctcgcccat caccacgcgc
    12241 ttgagcgcgc cgggcttgaa gatcgagaac acgttgatcg ggagcttctg gtcgcggcac
    12301 aacgcgaacg cggtcgagtc gagcacctgc agattgcgcc cgatcgcctc gtcgaagctg
    12361 atgcgatggt agcgctgccc ctgcgggtcc ttcttcggat ccgcagtata gacgccgtcc
    12421 accttggtcg ccttgagaac gatctgggca ccgatctcgg cgccgcgcag cgccgcggcg
    12481 gtgtcggtgg tgaagaaggg gttgccggta ccggccgcga agatgaccac gcggccttcc
    12541 tcaagatgac ggatcgcgcg cccgcggatg tagggctcga ccacctggtc gatgcgcagt
    12601 gcggactgca cgcgcgcttc gagccccatg cgtcgcatcg cgtcggcgag cgccatcgcg
    12661 ttcatcaccg tggccagcat gcccatgtag tcggcggtgg cgcggtccat gccggaggcc
    12721 gcgcccttca tgccgcggaa gatgttgccg ccgccgatca ccacaccgac ctggacaccg
    12781 agacggctga tctcggcgac ctcggacacg atgcggctga cgacctcctc gttgatgccg
    12841 taggcgtcgt cgcccatgag ggcttcgccg gagagcttga gcaggatgcg cttgtaggcg
    12901 gcggacacgt tcttctcctt acttcttggc ggcagcggcg gcctgttcgg ccacttcggc
    12961 ggcgaagtcg gtgaccttct tctcgatgcc ttcgccgacg atgaacagcg tgaagccggc
    13021 gaccgaggcg cccttggcct tgagcagctg ctcgatggtc tgcttgccgt cctcggcctt
    13081 cacgaagacc tgggccagca gggtcacttc cttgaggaac ttctgcacgg tgccgtcggc
    13141 gatcttttcc agcatcgctt cgggcttgtt gtcggcgcgg gccttctcga tcgcgatgcg
    13201 acgctcggca tcgatcaggt cctgcgacac gcccgaggcg tcgagcgcct tcggcttgga
    13261 cgcggcgatg tgcatcgcga tgtccttgcc gagctgctcg tcgccgccga ccacgtccag
    13321 cagcacgccg atcttggcgc cgccgtggat gtaggagaac agcttgccct tggcctcgat
    13381 gcgggcgaag cggcggatcg acatgttctc gccgatcttg ccgaccagcg cggtgcgggt
    13441 cgactcgacg gtgccctcgc ccagcggcag cgccgacagg gcagcgacgt cggccgggct
    13501 cttggcggcg acgaggccgg cgcagttctg caccaaggcc aggaagtcgt cgttcttggc
    13561 gacgaagtcg gtctcgcagt tgacctcgat gatcgagccg agcttgccgt cggccgagat
    13621 gtcgatgccg accacgcctt cggcggcgat gcgggcggcg gccttggagg ccttgttgcc
    13681 gagcttgacg cg
//
LOCUS       DAOQNN010000038        13652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1887_length_13652_cov_10.869971, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000038 DAOQNN010000000
VERSION     DAOQNN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 13652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13652
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1887_length_13652_cov_10.869971"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PKV97_04125"
     CDS             <1..1235
                     /locus_tag="PKV97_04125"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNB05095.1"
                     /translation="VTAGIFMVARMSPLFELSDTALSFVLVIGATTALFMGFLGIVQN
                     DIKRVVAYSTLSQLGYMTVALGVSAYSAAVFHLMTHAFFKALLFLGAGSVIIGMHHDQ
                     DMRNMGGLRKYMPITWITSLLGSLALIGFPFFSGFYSKDSIIEAVHSSTIAGSGYAMF
                     CVLLGVFITAFYSFRMYFLVFHGDERFGKAHDHAHHDHHGDHDDEEPSADHHHGLAPG
                     QKPHESPWVVTLPLVLLAVPSVLIGFFTIDPMLFGEWFKGVILVGDNHVGLRELAANY
                     HGPVGMAIHGLQTAPFWLAMGGVALAWFFYMKRPDIPAAIQRTFKPIHTLLENKYYFD
                     RFNEIFFAAGARLLGRGLWKAGDQALIDGVAVNGSARLVGWVAQMSRLFQTGHLYQYA
                     FMMIIGVFVLLTYWFNRG"
     gene            1310..2791
                     /locus_tag="PKV97_04130"
     CDS             1310..2791
                     /locus_tag="PKV97_04130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNB05096.1"
                     /translation="MTDIPLLSLAIWVPIIGGLLVLATGSDRNAPLARMLALAVAVAG
                     FVVTIPLYTGFDVGTSAMQFVELQSWVPRFNINYHLGVDGISVLFVLLNAFITIIVVA
                     AGWQVIQTKVAQYMAAFLIMSGLMNGIFSALDAVLFYVFFEASLIPLYLIIGIWGGAN
                     RVYAAIKFFLYTLFGSLLMLIALLYLFMQSGGSFSILDWHQLPLPIEPQILIFLAFLI
                     AFGVKVPMWPVHTWLPDAHVEAPTGGSVVLAAIALKLGAYGFLRFSLPIVPDAANELA
                     PLVITLSLIAVVYIGFVALVQADMKKLVAYSSISHMGFVTLGFFMFNSLGVEGALVQM
                     ISHGFVSGAMFLCIGVLYDRLHSRQIADYGGVVHTMPKFAAFFMLFTMANSGLPATSG
                     FVGEFMVVLGAVQYNFWIGFVAATTLILGAAYSLWMYKRVVFGKVANPHVAELEDING
                     REFAFLAVLAACVLAMGLYPFPFTEVMHASVNELLRHVAVSKL"
     gene            2840..4309
                     /gene="nuoN"
                     /locus_tag="PKV97_04135"
     CDS             2840..4309
                     /gene="nuoN"
                     /locus_tag="PKV97_04135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HNB05097.1"
                     /translation="MNFVVPDFYPAAAEIFVAVMALVIMLASTFARSVARNLAYLLTQ
                     ATLIAAAFITIFTMEGEVVHTFSNLFISDLLGDVLKLMIYFSTAIALLYGRGYLADRK
                     IDKPEYYLLALLMTLGMMVMVTANHMLPMYIGLEMMSLALYTMVAFDRDSARSTEAAM
                     KYFVLGALASGLLLYGMSMVYGATGTLEFSGVAQALYNQTANQTVLMFGVVFLVAGIC
                     FKLGVVPFHMWVPDVYQGAPTAVTLVIATAPKLAAFAMAVRLLIWALFDIAEEWQMML
                     MLVAAASIVLGNLAAIAQQNIKRMLAYSGISHMGFMLLGLLAGVVEGDRHFALNAYSS
                     AMFYAISYVIMSLASFGMLILLSRAGFEAENIDDFKGLNKRSSWYALMMLFVMFSMAG
                     VPFFIGFFAKLSVLQAVVAAGYFWLAVLAVVMSVIGAFYYLRVVKVMYFDEPVDSSPI
                     HAAPEVRVMLSANGLAIAALGLAPQALMSLCAFALLRSL"
     gene            4317..4649
                     /locus_tag="PKV97_04140"
     CDS             4317..4649
                     /locus_tag="PKV97_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HNB05098.1"
                     /translation="MGADASVFLLLAIAFTMANLPFVFDRIFLIRKPPAGVRKGLGWR
                     FAELLVLYFVVGGLAASIEAQSHGRVYGQGWAFYATTFCLFVVFAFPGFTWAYLWRSA
                     RRTSEVGA"
     gene            4646..5215
                     /locus_tag="PKV97_04145"
     CDS             4646..5215
                     /locus_tag="PKV97_04145"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNB05099.1"
                     /translation="MSGLERDALHEHTLDSESVFDGALLKVWRDRVELPDGSRSVREY
                     IRHPGAVVVVALEESGALVFERQFRYPLQRAFLELPAGKIDAGEDILTCAQRELREET
                     GFAAANWEYVGVMHPCIGYSDERIEVFFAHELSQVGGALDDGEFLEVLSLRADEVEQA
                     IRSGRITDAKTITAYYLALPHIQARLRGR"
     gene            complement(5247..6527)
                     /locus_tag="PKV97_04150"
     CDS             complement(5247..6527)
                     /locus_tag="PKV97_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB05100.1"
                     /translation="MSQPDGFHLIPRIARIDARQWDALVDASCASAAPCIRHAFLHAL
                     EESGCVGGRSGWTPAHATLWRGGTLGAAMPLYVKTHSRGEYVFDWAWAEAYQRHGLDY
                     YPKWLAAVPFTPIPGPRLLGHDDGARRELLAALLAKALESGLSSLHLLFPLDRERPLL
                     EAAGLMIREDVQFHWRNPPASAETEPTPAESSAPISVRPRRWADFEHFLASLSGAKRK
                     KIRQERRRAAAHGLDLQWLDGHEARDEDWAFFAHCYANTYAQHRSTPYLAADFFVRLA
                     HSMPEAVRLLVARRDTQPIAAAFFLCDREALYGRYWGCVEDLPFLHFELCYYQAIEYC
                     IEHGLTRFEGGAQGEHKLARGLLPVRTFSAHWLADPRFRGAVDDWLARERAGVGHYLD
                     ELAEHSPFLREKGPGGIETGAAGAAKPQRGSRRQ"
     gene            6823..8451
                     /locus_tag="PKV97_04155"
     CDS             6823..8451
                     /locus_tag="PKV97_04155"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNB05101.1"
                     /translation="MNAHAQAPVSIAVAQLNLTVGDLVGNADHIIAALDDARAAGAQL
                     LLTPELALSGYPPEDLLLRPDFYRACAREVERIAAAARGVTVVLGHPVEDGGERYNAA
                     SVLRDGAVLTRYHKSLLPNYEVFDEERYFEAGGTACVFECAGVRFGVNICADVWERGP
                     AEAARAAGAQVLLALNASPYHMNKQAQRLEVLRARVAETGLPVLYCNMVGGQDELVFD
                     GASFALDAGGAVGLQLGSFVERVAIVDYASGGWSGGEHVPARTLEAEVYEALRIGVRD
                     YLAKNRFPGAIIGLSGGIDSALTLAIAVDALGADKVRAVMMPSPYTAKMSLDDSRDMV
                     ARLGVRYDEIAIEPAMKVFAELLADQFAGLPADTTEENLQARIRGMLLMALSNKTGAI
                     VLTTGNKSEMATGYATLYGDMAGGFAVLKDLYKTFVFRLSNWRNTVSPVIPQNIIDRP
                     PSAELKPDQKDQDSLPPYDMLDAIIQAYMERDESPRDIVAAGFPEGEVRRVVGMLKRN
                     EYKRRQAPVGIRVTQRGFGRDWRYPITSRYQDEF"
     gene            8468..8806
                     /locus_tag="PKV97_04160"
     CDS             8468..8806
                     /locus_tag="PKV97_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNB05102.1"
                     /translation="MKKIEAIIKPFKLDEVREGLSEVGITGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIKVEVVLADDIVDQAVEAIVKAAHTGKIGDGKIFITPVEQVIRI
                     RTGETNEAAI"
     gene            complement(8824..9930)
                     /locus_tag="PKV97_04165"
     CDS             complement(8824..9930)
                     /locus_tag="PKV97_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNB05103.1"
                     /translation="MSRRAPAPGPAGTPGRDAQGAVPKPASPFAALRKQLQQRALPAP
                     VSSPAPAKRIRHPLEEDSSSAGADRAQEPDAEALELFRRSVGAVRPVRGTDRVEIHRP
                     RPAPRPRTQAVEDEETAEPVRARPETDPLRAAYEGVMPLRDTGRVALDTPLRHHARHA
                     GGTHPAPALRPDAIVLPADADVSDPAALFRAVVGSARPVTDRNRVELERPQPAPAPLK
                     REEDERAALGESLAAPLTFEDRLDMGDEAAFLRTGLPRRVLTDLRRGRWVLQGQIDLH
                     GLTRDEARAALANFLHDALAQGKRCIRVIHGKGHGSPGKVSILKQLSRGWLAQREEIL
                     AFCQAGPHDGGGGALLVLLRAQNAAPRARMPLPA"
     gene            complement(9939..10892)
                     /gene="trxB"
                     /locus_tag="PKV97_04170"
     CDS             complement(9939..10892)
                     /gene="trxB"
                     /locus_tag="PKV97_04170"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNB05104.1"
                     /translation="MTTKHARLLILGSGPAGYTAAVYAARANLNPVLITGLAQGGQLM
                     TTTEVDNWPADADGVMGPDLMARFQRHAERFNTEMIFDHIHTVKLGEKPFRLIGDSGE
                     YTCDALIISTGATAKYLGLPSEEKFAGRGVSACATCDGFFYRNQDVAVIGGGNTAVEE
                     ALYLANIARKVTLVHRREKFRAEKIMIDKLMEKVAAGRIELALNATLDEVLGDNTGVT
                     GMRIKGVNNGATREVALQGVFIAIGHKPNTDIFEGQLEMEGGYIVTQGGRNGNATQTS
                     VPGVFAAGDVQDHIYRQAVTSAGTGCMAALDAERYLDALGV"
     gene            11162..11833
                     /locus_tag="PKV97_04175"
     CDS             11162..11833
                     /locus_tag="PKV97_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB05105.1"
                     /translation="MTQATAVSVVALKTFPLFHGLSDESLASIARVSMMRRFPRGQSV
                     VCAGDRPDYVYLVLTGSLKVVVSDEDGREVILSILGQGELFGEMGMFDERPRSASVIA
                     VVPADLVLISKQDFRRIMQDSFDVSWRIMCNLAERLRNADRKIESLALMDVYGRVARL
                     LIEMAEDVGGQSVVVRRISKQDIAKMIGASREMVSRVMKDLGQQGLIEEGPDGILLRE
                     RLNDV"
     gene            11944..>13652
                     /locus_tag="PKV97_04180"
     CDS             11944..>13652
                     /locus_tag="PKV97_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNB05106.1"
                     /translation="MLSRSSSRSQPLPEKISLLLQEARWLILGVVSLYVGMILVGYSE
                     SDPGWSHASDVTRVANPGGRFGAWLADLLLYLFGISSWWWVVFLGYSLLWGFRRLKNR
                     LELDHRSFIFVMIGFFVVLITSSALEFLRFHSHGAALPLEPGGLIGMELAQFVRRSFG
                     FTGGTLLLLALLATGLSLFTGVSWLALMERIGLGIEQAVLGVQQAWLRWQDRRAGREV
                     AHKREEVVQTRRKKVEKAEPAPLRIEPAVVAVTKSERVEKERQQTLFQDAVEGVIPPV
                     ALLDPASGGVEPPSPESLEFTSRLIETKLADFGVEVKVLAAYPGPVITRYEIEPATGV
                     KGSQVVNLAKDLARALSLVSIRVVETVPGKSCMALELPNPKRQMVRLSEIIGSKVYQD
                     AHSPLTVVLGKDIGGQPVVADLAKMPHLLVAGTTGSGKSVGINAMILSLLYKSEPDRV
                     RLIMVDPKMLELSIYEGIPHLLAPVVTDMKHAGNALNWCVAEMDKRYKLMAAVGVRNL
                     AGFNKAVQEARKAEQPLTNPFSISPENPEPLEPLPYIVVVVDELADMMMVVGKKVEEL
                     IARL"
BASE COUNT         2018 a         4406 c         4651 g         2577 t
ORIGIN      
        1 tggtgaccgc gggcatcttc atggtggcgc gcatgtcgcc gctgttcgag ctgtccgaca
       61 ccgcgctgtc cttcgtcctc gtgatcggtg cgaccaccgc gctcttcatg ggcttcctcg
      121 gcatcgtgca gaacgacatc aaacgcgtcg tggcctactc gacgctgtcg cagctcggct
      181 acatgacggt cgcgctcggc gtctcggcct actcggccgc ggtcttccac ctgatgacgc
      241 acgccttctt caaggcgttg ctgttcctcg gcgccggctc ggtgatcatc ggcatgcacc
      301 acgatcagga catgcgcaac atgggcggct tgcgcaagta catgccgatc acctggatca
      361 cctcgctcct gggttcgctt gcgctgatcg gttttccgtt cttctcgggc ttctactcca
      421 aggactcgat tatcgaagcg gtgcattcct cgacgatcgc gggctcgggc tatgcgatgt
      481 tctgcgtgct cctcggggtg ttcatcaccg cgttctactc cttccggatg tacttcctcg
      541 tgttccacgg cgacgagcgc ttcggcaagg cgcatgatca cgcacatcac gatcatcacg
      601 gcgaccacga cgacgaggaa ccctcggcgg atcatcacca cggcctggcg ccggggcaga
      661 agccgcacga gtcgccctgg gtggtgaccc tgccgctggt gctgctggcg gtgccctcgg
      721 tcctgatcgg cttcttcacc atcgacccga tgctgttcgg cgagtggttc aagggtgtga
      781 tcctcgtcgg cgacaaccac gtcggcctgc gggagctcgc agccaactac cacggcccgg
      841 tcggcatggc gatccacggt ctccagacgg cgccgttctg gctggcgatg ggcggagtcg
      901 cgcttgcctg gttcttctac atgaagcgtc cggatatccc ggcggcgatc cagcgcacct
      961 tcaagccgat ccacacgctg ctggagaaca agtactactt cgatcgcttc aacgagatct
     1021 tcttcgccgc aggtgcgcgt ctgctcggac gcggtctgtg gaaggcgggc gatcaggccc
     1081 tgatcgatgg tgtcgcggtg aacggctccg cgcgcctggt gggctgggtg gcgcagatgt
     1141 cgcgcctctt ccagacgggt cacctgtacc agtacgcctt catgatgatc atcggcgtgt
     1201 tcgtgctgct cacctactgg ttcaaccggg gctgagacgg gcacgcaggg acggaaacac
     1261 ataacaagat cgaacgcgcc gctcgcggcg cactgaacgg aaagcaacga tgacggacat
     1321 tcctctcctc agtctggcga tctgggtacc gattatcggt ggcctgctgg tgctcgcgac
     1381 cggctcggat cgcaacgctc cgctggcgcg catgctcgcc ttggcggtcg ccgtggctgg
     1441 cttcgtcgtg acgattccgc tctacacggg cttcgacgtc gggaccagcg cgatgcagtt
     1501 cgtggaactg cagtcctggg tgccgcgctt caacatcaac taccacctcg gcgtcgatgg
     1561 catctcggtg ctgttcgtgc tgctcaacgc cttcatcacc atcatcgtgg tggcggcggg
     1621 ctggcaggtg atccagacca aggtcgcgca gtacatggcc gcgttcctga tcatgtcggg
     1681 cctgatgaac ggcatcttct cggcgctcga cgcggtgctg ttctacgtgt tcttcgaagc
     1741 ctcgctgatc ccgctctacc tgatcatcgg catctggggc ggcgccaacc gggtgtacgc
     1801 cgcgatcaag ttcttcctct acacgctgtt cggttccctg ctgatgctga tcgcgctgct
     1861 ctacctcttc atgcagtcgg gcggcagctt cagcatcctc gactggcacc agttgcccct
     1921 gccgatcgag ccgcagatcc tcatcttcct cgccttcctg atcgcttttg gcgtcaaggt
     1981 gccgatgtgg ccggtgcata cttggctgcc cgatgcccac gtcgaggcac ccaccggcgg
     2041 atcggttgta cttgccgcga tcgccctgaa gctcggcgct tatggcttcc tgcgcttctc
     2101 cctgccgatc gtgcccgacg cggcgaacga gctcgcgccg ctggtgatca ccctgtcgct
     2161 gatcgcggtt gtgtatatcg gcttcgtcgc gctggtacag gccgacatga agaagctcgt
     2221 ggcctactcg tcgatctccc acatgggctt cgtgaccctg ggcttcttca tgttcaacag
     2281 cctcggcgtc gagggcgctc tggtgcagat gatctctcac ggcttcgttt cgggcgcgat
     2341 gttcctgtgc atcggcgtgc tctacgaccg cctgcactcg cgccagatcg ccgactacgg
     2401 tggcgtggtg cacaccatgc cgaagttcgc agccttcttc atgctcttca cgatggccaa
     2461 ctctggcctg ccggccacct ccggtttcgt cggcgagttc atggtggtgc ttggggccgt
     2521 gcagtacaac ttctggatcg gcttcgtcgc cgccaccacg ctgatcctcg gcgctgcgta
     2581 ttcgctctgg atgtacaagc gggtggtgtt cggcaaggtg gccaaccccc atgtcgccga
     2641 gctcgaggac atcaacggcc gcgagttcgc cttccttgcg gtgctggcgg cgtgcgtgct
     2701 ggcgatgggc ctgtatccct tccccttcac cgaagtgatg cacgcctcgg tcaatgaact
     2761 cctgcggcat gttgccgtga gcaagctctg agcgctgccc ggcgcgagcg cacgagacac
     2821 tttacggact ggtcagaaga tgaatttcgt cgtccccgac ttctaccccg cagcggccga
     2881 gatcttcgtg gccgtgatgg ccttggtgat catgctggcg agtaccttcg cgcgcagcgt
     2941 cgctcgcaac ttggcttatc tgcttactca ggcgaccctg atcgccgcgg ccttcatcac
     3001 gatcttcacg atggaggggg aggtggtcca taccttcagc aatctgttca tcagcgatct
     3061 gctgggtgac gtgctgaagc tgatgatcta tttctccacc gcgatcgcgc tgctgtacgg
     3121 gcgtggctac ctcgccgacc ggaagatcga caagcccgag tactacctgc tcgcgctgct
     3181 gatgacgctc ggcatgatgg tgatggtcac ggccaaccac atgctgccga tgtacatcgg
     3241 cctcgagatg atgtcgctag cgctgtatac gatggtggcg ttcgatcgcg actcggcgcg
     3301 ctcgaccgag gcggcgatga agtacttcgt actcggcgcg ctggcttcgg gcctgctgct
     3361 gtacggcatg tcgatggtgt atggcgcgac cggtacgctc gagttctcgg gtgtggcgca
     3421 ggcgctctac aaccagaccg ccaaccagac cgtgctgatg ttcggcgtcg tgttcctggt
     3481 cgcgggtatc tgcttcaaac tcggcgttgt gcccttccac atgtgggtgc ccgacgtcta
     3541 tcagggcgcg cccaccgcag tgacgctggt gatcgccacg gcgcccaagc tcgccgcgtt
     3601 cgcgatggcg gtgcgcctgc tgatctgggc gctgttcgac atcgccgagg aatggcagat
     3661 gatgctgatg ctggtggccg cagcctcgat cgtgctcggc aatctggccg cgatcgcgca
     3721 gcagaacatc aaacgcatgc tcgcctactc gggtatctcg cacatgggct tcatgctgct
     3781 cggcctgctc gcgggcgtgg tcgagggcga ccgtcacttt gcgctcaatg cctacagctc
     3841 ggcgatgttc tacgcgatct cttacgtgat catgagcctg gcttcgttcg gcatgctgat
     3901 tctgctgtcg cgtgcaggtt tcgaggccga gaacatcgac gatttcaagg ggctcaacaa
     3961 gcgcagttcg tggtacgcgc tgatgatgct cttcgtgatg ttctcgatgg cgggtgtgcc
     4021 cttcttcatc ggcttcttcg ccaagttgtc ggtgctgcag gcggtggtcg cggccgggta
     4081 cttctggctg gcggtgctgg cggtggtgat gtcggtgatc ggtgccttct actacctgcg
     4141 cgtggtcaag gtgatgtatt tcgacgagcc agtcgactcc tcgccgatcc atgctgcgcc
     4201 cgaagtgcgt gtgatgctct cggccaacgg tcttgcgatc gcggccctcg gcctcgcccc
     4261 gcaggcgctg atgtccttgt gtgcgttcgc gctgctgcgt tcgctctgat ccagcggtgg
     4321 gtgcggacgc cagcgtcttc ctgctgttgg ccatcgcctt cacgatggcc aacctgccct
     4381 tcgtcttcga ccgcatcttc ctcatccgca agccgccagc cggggttcgc aaggggctcg
     4441 gctggcggtt tgccgaactg ctcgtgctgt atttcgtggt gggtgggctc gcagcatcga
     4501 tcgaggcgca gtcccacggt cgggtgtatg ggcaggggtg ggcgttctac gcgaccacct
     4561 tctgtctctt tgtggtgttt gcctttccgg gcttcacttg ggcctacctg tggcgttcgg
     4621 cgcgccggac atccgaggta ggtgcgtgag cgggctcgag cgggacgcgc tacatgagca
     4681 caccctcgat agcgagtcgg ttttcgacgg tgcactgctc aaggtttggc gagaccgcgt
     4741 cgaattgccg gacggcagcc gcagcgtgcg cgagtacatc cgccatcccg gcgcggtggt
     4801 cgttgtcgcg ctggaggaga gtggggcgct ggtgttcgag cggcagttcc gctatccgct
     4861 gcaacgggcc ttcctcgagt tgcctgccgg gaaaatcgac gccggtgagg acatcctgac
     4921 ctgcgcgcag cgcgaactgc gcgaggaaac cggcttcgcc gctgcgaatt gggaatatgt
     4981 cggggtgatg cacccgtgta tcggctactc ggacgagcgc atcgaagtct tcttcgccca
     5041 cgagctctcg caggtggggg gggcgctcga tgacggcgag tttctcgaag tgctgagcct
     5101 gcgtgcggac gaggtggagc aggccatccg gagcggacgc atcaccgacg cgaagaccat
     5161 caccgcttat taccttgcgt tgccgcatat ccaggcgcgt ttgcggggac gctagcgcgc
     5221 ttcgccgccg ggtcgcgctt ctctcgtcac tgccggcgtg aaccccgctg gggcttcgcg
     5281 gctcctgctg cgccggtctc gatgccgccg ggtcccttct cgcgaaggaa tgggctgtgc
     5341 tccgccagtt cgtcgagata gtgccccacg cccgcacgct cccttgccag ccaatcgtcg
     5401 accgcgccgc gaaagcgcgg atccgcgagc cagtgcgcgg agaaggtacg caccgggagc
     5461 agaccgcggg cgagcttgtg ctccccctgt gcgccgccct cgaagcgggt cagaccgtgc
     5521 tcgatgcagt attcgatggc ctggtagtag cacagctcga agtgcaggaa gggcaggtcc
     5581 tcgacgcagc cccagtaacg cccgtacagc gcctcgcgat cgcacaggaa gaaggcggcg
     5641 gcgatcggct gggtgtcgcg gcgagccacc agcagacgga cggcctcggg catgctgtgc
     5701 gcgagccgca caaaaaaatc tgctgcgaga tagggcgtgg agcggtgctg cgcgtaggtg
     5761 ttggcgtaac agtgcgcgaa gaacgcccag tcctcgtccc gcgcttcatg cccgtccagc
     5821 cactgcagat ccagaccgtg tgcggctgcg cggcggcgtt cctggcggat cttcttgcgc
     5881 ttggcgccgg acaggctggc gaggaaatgc tcgaagtcgg cccagcgccg cggccggacg
     5941 gagataggtg cgctggattc cgcgggcgtc ggctcggtct cagcggaggc gggcggattt
     6001 ctccaatgga actgcacgtc ctcgcggatc attagcccgg cggcctcgag cagcgggcgc
     6061 tcgcgatcga gcgggaaaag caggtgcagc gacgacagcc cgctctccag cgccttcgcg
     6121 agcagggcgg cgagcagttc ccggcgtgcg ccgtcgtcgt ggccgagcag gcgtggcccg
     6181 gggatgggcg tgaagggcac ggccgcgagc cacttcgggt agtagtcgag gccgtggcgc
     6241 tggtaggcct cggcccaggc ccagtcgaag acgtactcgc cccgggaatg cgtcttcaca
     6301 tagagcggca tggccgcgcc caacgtgccg ccgcgccaca gggtggcgtg cgcgggcgtc
     6361 cagccgctgc gcccgcccac gcagccgctc tcttcgaggg cgtgcaggaa ggcgtggcgg
     6421 atacagggcg cggcgcttgc gcagctcgcg tcgaccaggg catcccactg gcgtgcgtcg
     6481 atacgggcga tgcgggggat gaggtggaat ccgtcgggct ggctcatggg ggctggggca
     6541 ggggcacggc tgcctgtttt ttgtgcagtg cgacgatacg ttgccgcgac agcgacttgc
     6601 aagcgctatc cacgccttgt ccacaggctt gcgcacccgg gctgtgggtt tgtacgcggg
     6661 catccacggt cgcgcttcgc tcgcgagacg gccgcacggt tcgagtcggg gcaaaccatc
     6721 gcgtagcgga gcgcgcttgg gcgcgagagc gtgctcaggc gcgcggtccg gcgcacgcag
     6781 agcctgtcgt ggagcttcgc cgaagcggta tagttccgct ccatgaacgc acatgcccaa
     6841 gcccccgttt ccatcgctgt cgcccagctc aacctgaccg tgggcgatct ggtcggcaac
     6901 gccgaccaca tcatcgccgc gctcgacgac gcacgcgcgg ccggcgcgca actgctgctc
     6961 acgcccgagc ttgcgctgtc cgggtatccg cccgaggact tgctgctgcg accggatttc
     7021 tatcgcgcct gcgcgcgtga ggtcgaacgc atcgctgctg ctgcgcgggg ggtcacggtg
     7081 gtgcttggcc acccggtgga ggacggcggc gagcgctaca atgcggcctc ggtgttgcgt
     7141 gacggcgcgg tgctgacgcg ctatcacaag agcctgctgc ccaactacga ggtcttcgac
     7201 gaggagcgct acttcgaggc cggcggcacg gcttgcgtgt tcgagtgcgc tggcgtgcgc
     7261 ttcggggtga acatctgcgc cgacgtctgg gagcgcggtc ccgcggaggc cgcgcgggcg
     7321 gccggggcgc aggttctgct ggcgctcaac gcttcgccct accacatgaa caagcaggct
     7381 cagcgcctgg aggtgctgcg cgcgcgcgtg gccgagaccg gcctgcccgt gctgtactgc
     7441 aacatggtgg gcggccagga cgagttggtc ttcgacggcg cctccttcgc gctcgacgcc
     7501 ggcggcgcgg tcgggctgca actcggcagc ttcgtcgagc gcgtcgcgat cgtcgactac
     7561 gcctcgggcg gttggtcggg cggtgagcac gttcctgcgc ggacgctcga ggccgaggtg
     7621 tacgaggcgc tgcgcatcgg ggtgcgcgac tatctcgcca agaaccgttt cccgggcgcg
     7681 atcatcggcc tgtcgggcgg gatcgactcg gcgctgaccc tggcgatcgc ggtcgatgcg
     7741 ctcggcgccg acaaggtgcg tgcggtgatg atgccttcgc cctacaccgc taaaatgagc
     7801 ctcgacgact cgcgcgacat ggtggcgcgc ctgggcgtgc gctatgacga gatcgcgatc
     7861 gagccggcga tgaaggtctt tgccgaactg cttgccgacc agtttgccgg cctgcccgcc
     7921 gacaccaccg aggagaacct gcaggcgcgc attcgcggca tgctgctgat ggcgctgtcg
     7981 aacaagaccg gcgcgatcgt gctcaccacc ggcaacaaga gcgagatggc caccggctac
     8041 gccacgctct acggcgacat ggccggcggc ttcgccgtgc tcaaggacct ctacaagacc
     8101 tttgtcttcc gcctgtcgaa ctggcgcaat acggtgagcc cggtgatccc gcagaacatc
     8161 atcgaccgac cgccctcggc cgagctcaag cccgaccaga aggaccagga cagcctgccg
     8221 ccctacgaca tgcttgacgc catcatccag gcctacatgg agcgcgacga gtcgccgcgc
     8281 gacatcgtcg ccgcgggctt ccccgagggc gaggtgcggc gcgtggtggg tatgctcaag
     8341 cgcaacgagt acaagcgccg ccaggccccg gtgggcatcc gtgtgaccca gcgcggtttc
     8401 ggcagggatt ggcgttatcc gataacatcc cgctaccagg acgaattctg aaagaggagc
     8461 aagccggatg aagaagatcg aggcgatcat caagccgttc aagctggacg aggtgcgcga
     8521 gggcctgtcc gaggtgggga tcacgggcct gacggtgacc gaggtcaagg gtttcggccg
     8581 ccagaagggc cacaccgagc tctatcgcgg tgctgaatac gtggtcgact tcctgcccaa
     8641 gatcaaggtc gaggtcgtgc ttgccgatga catcgtcgac caggcggtgg aggcgatcgt
     8701 gaaggccgcg cacacgggca agatcggcga cggcaagatc ttcatcacgc cggtggagca
     8761 ggtcatccgc atccgcactg gcgagaccaa cgaagcggcg atctgatatt cgggcggcgg
     8821 cactcaggcg ggtaacggca ttcgggcgcg cggcgcggcg ttctgcgcgc gcagcagcac
     8881 cagcagggcg ccgccgccgc catcgtgggg accggcctgg caaaaggcga ggatctcctc
     8941 gcgctgcgcc agccagccgc gcgacagctg tttcaggatc gacaccttcc cgggcgagcc
     9001 gtggcccttg ccgtggatca cccggatgca gcgcttgccc tgggcaagcg cgtcgtgcag
     9061 gaagttcgcc agcgcggcgc gcgcctcgtc gcgggtgagg ccgtggaggt cgatctggcc
     9121 ctgcagcacc cagcgcccgc ggcgcagatc ggtcagcacc cggcgcggca gcccggtccg
     9181 caggaaggcc gcctcgtcgc ccatgtccag gcgatcctcg aaggtgagcg gtgcggcgag
     9241 cgattcgccg agcgccgcgc gctcgtcctc ctcgcgcttg agcggcgcag gtgccggctg
     9301 cgggcgctcc agctccacgc ggttgcggtc ggtgaccggg cgggcactgc ccaccaccgc
     9361 acgaaagagc gctgccggat cgctgacgtc ggcatccgca ggcagcacga tcgcgtcggg
     9421 tcgcagcgcc ggcgcgggat gcgtaccgcc tgcatggcgg gcgtggtgac gcagcggtgt
     9481 gtcgagcgcc actcggccgg tatccctcag cggcatcacg ccctcgtagg ccgcgcgtag
     9541 cgggtcggtc tcgggtcgcg cacggacggg ctccgcggtt tcctcgtcct ccacggcttg
     9601 cgtgcgcggc cgcggggcag ggcgggggcg gtggatctcg acgcggtccg tgccgcgcac
     9661 cgggcgcacc gcgccgacgc tgcgccggaa gagctcgagc gcctccgcgt cgggctcctg
     9721 tgcacgatcg gcaccggcgc tggaggagtc ttcctcaagc gggtggcgga tacgcttcgc
     9781 cggggcgggc gacgacacgg gtgccgggag cgcgcgctgc tgcagctgct tgcgcagggc
     9841 tgcgaagggc gaggcgggct tcggcaccgc gccctgcgcg tcgcgtcctg gggtgccggc
     9901 cggacccggc gcgggagcgc ggcggctcat cggcctgctc agacgccgag ggcgtcgagg
     9961 tagcgctcgg cgtcgagtgc ggccatgcag ccggtgccgg ccgaagtcac cgcctggcgg
    10021 taaatgtggt cctgcacgtc gccggcagcg aacacgccgg gaacgcttgt ctgcgtcgcg
    10081 ttgccgttgc gcccgccctg ggtgacgatg tagccgccct ccatctcgag ctggccttcg
    10141 aaaatgtcgg tgttgggctt gtggccgatg gcgatgaaca cgccctgcag cgccacctcc
    10201 cgggtggcgc cgttgttcac gcccttgatg cgcatccccg tcacgccggt gttgtcgccc
    10261 agcacctcgt cgagcgtggc attgagcgcg agctcgatcc tgccggcggc gaccttctcc
    10321 atcagcttgt cgatcatgat cttctcggcg cggaacttct cgcggcggtg caccagggtg
    10381 acctttctcg cgatgttggc gaggtagagc gcctcctcga ccgcggtgtt gccgccgccg
    10441 atcaccgcga cgtcctggtt gcggtagaag aagccgtcgc aggtggcgca cgccgacacg
    10501 ccgcgcccgg cgaatttttc ctccgagggc aggccgaggt acttggcggt ggcgccggtg
    10561 gagatgatca gcgcatcgca ggtgtattcg ccgctgtcgc cgatcaggcg gaagggcttc
    10621 tcgcccagct tcacggtgtg gatgtggtcg aagatcatct cggtgttgaa gcgctcggcg
    10681 tgcctctgga agcgcgccat caggtccgga cccatcacgc cgtctgcatc ggccggccag
    10741 ttgtcgacct cggtcgtggt catgagctgg ccgccctggg cgaggccggt gatcagcacc
    10801 gggttcaggt tggcgcgcgc ggcatagacg gcggcggtgt agccggcggg gccggagccg
    10861 aggatcagca gacgggcgtg tttggtggtc atggacgagg cttcctgcgg gtggattgcg
    10921 aggcgccgat tatagcggag ggggcggcgg gggttggagg tggtcgccgg agtccgggcg
    10981 ctcgcgcgtg attccgatgg ggagacgcgc gttcctgtct gtggtgcagc gacacgcatc
    11041 gaactcccgc tggatgcgga aagtgtgatc tccgtcacga aatcgagaat aagcccgggg
    11101 tgatcgtata aacttgaaaa tgcatgtttc aactgttcca ctcctcgtgc ctggagcgcg
    11161 catgacccaa gccaccgccg tttccgtcgt cgccctgaag accttcccgc tgttccatgg
    11221 cctgtccgac gagtcgttgg ccagcatcgc gcgcgtgtcg atgatgcgtc gcttcccccg
    11281 cgggcagtcc gtggtgtgcg cgggcgaccg tcccgactac gtctatctcg tgctcaccgg
    11341 cagcctcaag gtcgtggtga gcgacgagga cggccgcgag gtaatcctgt ccatcctcgg
    11401 ccagggcgag ctcttcggcg agatgggcat gttcgacgag cggccgcgct cggcctcggt
    11461 gatcgccgtg gtgccggccg atctggtgct gatctccaag caggatttcc gccgcatcat
    11521 gcaggacagc ttcgacgtgt cctggcgcat catgtgcaac ctcgccgagc gcctgcgcaa
    11581 cgccgatcgc aagatcgaga gcctcgcgct catggatgtc tacggccgtg ttgcgcgcct
    11641 gctgatcgag atggccgagg acgtcggcgg ccagtcggtg gtggtccggc gcatctccaa
    11701 gcaggacatc gccaagatga tcggcgcctc gcgcgagatg gtgagccggg tgatgaagga
    11761 cctcggccag cagggtctga tcgaggaagg gcccgacggc atcctgctgc gtgagcgcct
    11821 caacgacgtg tgaacgcgcg ctcgccgctc ggccgtttgt gagcatccgc aagcgcggcg
    11881 gcggggtggg gaccgatccc gtataatccg gggttgttcc actctccctg cggccgcaac
    11941 gcaatgctgt ctcgttcgtc ctcccgttcc cagccgctgc ccgaaaagat ctcgctgttg
    12001 ctgcaggagg cccgctggct gatcctcggc gtggtctcgc tctacgtcgg catgatcctc
    12061 gtcggctaca gcgagtccga cccaggctgg tcgcacgcct ccgacgtcac ccgcgttgcc
    12121 aatccgggcg ggcgtttcgg cgcttggctg gcggacctcc tgctctatct cttcggcatt
    12181 tcctcgtggt ggtgggtggt cttcctcggc tacagcctgc tgtggggttt ccgccgcctc
    12241 aagaacaggc tcgagctcga ccaccgctcc ttcatcttcg tgatgatcgg cttcttcgtg
    12301 gtgctgatca ccagcagcgc gctcgaattc ctgcgtttcc actcccacgg tgcggcgctg
    12361 ccgctcgagc ccggcggctt gatcggcatg gaactcgccc agttcgtgcg gcgcagcttc
    12421 ggcttcaccg gcggcacgct gctgctgctc gcgctgctgg ccacggggct gtcgctgttc
    12481 accggggtgt cgtggctggc tctgatggag cgcatcggtc ttggcatcga gcaggcggtg
    12541 ctcggcgtgc agcaggcttg gctgcgctgg caggaccgcc gtgcgggtcg cgaggtggcg
    12601 cacaagcgcg aggaggtggt gcagacccgg cgcaagaagg tcgagaaggc cgagcccgcg
    12661 ccgctgcgca tcgagccggc ggtggtcgcc gtgaccaagt ccgagcgggt ggagaaggag
    12721 cgccagcaga ccctgttcca ggatgcggtc gagggcgtga tcccgccggt ggcactgctc
    12781 gatccggcca gcggtggcgt ggagccgcct tcgcccgaat ccctcgagtt cacctcgcgc
    12841 ctgatcgaga ccaagctcgc cgacttcggc gtcgaggtca aggtgctcgc cgcttacccc
    12901 ggcccggtga tcactcgcta cgagatcgag ccggcaaccg gggtcaaggg cagccaggtg
    12961 gtgaaccttg ccaaggacct cgcgcgcgcg ctgtcgctgg tgtcgatccg cgtcgtcgag
    13021 accgtgccgg gcaagtcctg catggcgctc gagctgccca acccgaagcg gcagatggtg
    13081 cgcctgtcgg agatcatcgg ttccaaggtg taccaggacg cgcattcgcc gcttaccgtg
    13141 gtgctcggca aggacatcgg tggccagccg gtggtggccg acctggccaa gatgccacac
    13201 ctgctggtgg cgggtacgac gggctcgggc aaatcggtcg gcatcaacgc gatgatcctg
    13261 tcgctgctct acaagagtga gcccgatcgg gtgcgcttga tcatggtcga cccgaagatg
    13321 ctcgagctgt cgatctacga gggcatcccg cacctgctcg cacccgtcgt caccgacatg
    13381 aagcacgccg gcaacgcgct caactggtgc gtggcggaga tggacaagcg ctacaagctg
    13441 atggcggcgg tgggcgtgcg caacctcgcc ggcttcaaca aggccgtgca ggaggcgcgc
    13501 aaggccgagc agccgctcac caacccattc tcgatcagcc ccgagaaccc ggagccgctc
    13561 gagcccttgc cctacatcgt ggtggtggtc gacgagcttg ccgacatgat gatggtggtc
    13621 ggcaagaagg tcgaggagct gatcgcccgc ct
//
LOCUS       DAOQNN010000039        13449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_1932_length_13449_cov_9.129162, whole genome shotgun sequence.
ACCESSION   DAOQNN010000039 DAOQNN010000000
VERSION     DAOQNN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1932_length_13449_cov_9.129162"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PKV97_04185"
     CDS             <1..1033
                     /locus_tag="PKV97_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNB05107.1"
                     /translation="VQEPDEALRWLAPHLPALLRSVELCALPALPARALPEPDRRFDA
                     GVPGRKRAQIEAFAARLPATGLPLLEWCAGKGHLGRRLALADGVPVRSLEIDPALCAD
                     AERLAERSRLPQRVVCADALDPASRPHLHGHAVLALHACGELHRSLVRSADRDDAAAY
                     AIAPCCYHRGANGGYRALGRDASLELDAATLRLAVTETVTAPRHDRRRLARDQAWKLG
                     FIALRQTAEGGPVPHFRPVPPAWLNGSFEDFCRRLAEREGLRLPPALDWAEWEAVGER
                     RRDEVRRLELVRHAFRRALEVWLALDLVLGFEARGFTARIGTFCERALTPRNLLILAS
                     RRGEGPLQP"
     gene            complement(1024..2283)
                     /locus_tag="PKV97_04190"
     CDS             complement(1024..2283)
                     /locus_tag="PKV97_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNB05108.1"
                     /translation="MTQPPRACGIDFGTSNSTVGWLRPDAPILVPLEDGRATLPSAVF
                     FNADEDSIQFGRAALAEYLEGYEGRLMRALKSLLGTSLIDGQTEVNGRALGFRELLAS
                     FIGELKARAERATGRTFDHAVFGRPVHFVDEDETADRKAQDTLEAIARQVGFREIGFQ
                     FEPIGAALHYERTLSREELVLIADIGGGTADFSIVRLSPQRARAVERREDLLGNAGIH
                     IGGTDFDRVLSLDCVMPVLGYRSRLRNGATMPASVYFQLAAWHTINFAYTRQVWADLQ
                     QVYRDALARTELDRLCRLINLREGHWLAMQVEEAKIALSAAERTQVVLDRIEPGLVLE
                     LSRAAFTAATQGLVERVGGCVAGLLAASGVDRERIDTLYFTGGASGVPQLRARIAAEL
                     PRARCVEGDLYGSIGAGLAVEAARRYG"
     gene            2469..2975
                     /locus_tag="PKV97_04195"
     CDS             2469..2975
                     /locus_tag="PKV97_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNB05109.1"
                     /translation="MNSKDTTPPASTFSTPIDDRYFEDYVPGDVHRFGEMRVTEQEII
                     EFARRYDPQDFHTDPAKAADTQFGGLIASGWMTCGMMMRMYSEHYLTHNASLASPGID
                     ELRWLEPVRPDDVLSTRVTIVEARRSASKPDRGLVRSKIEVLNQHDRVVLSMLAMNLI
                     GCRTPAAA"
     gene            complement(3060..3671)
                     /locus_tag="PKV97_04200"
     CDS             complement(3060..3671)
                     /locus_tag="PKV97_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB05110.1"
                     /translation="MEAVAVIDFETTGLSPAQGDRATEIAAVIVRDGRVVDRYQSLMN
                     AGVRIPAHIESLTGISNAMIRSAPTAAQVMREVADFIGGIPMVAHNAAFDAKFWDAEL
                     ARIERARKQDFVCSLLLSRRLFPLAPSHRLGALVEYARLPVAGRFHRALADAEMAASL
                     LLRLEEELRQRHRVAQVSVELLCRIQRASKAQLPKCLAAAREG"
     gene            complement(3689..3979)
                     /locus_tag="PKV97_04205"
     CDS             complement(3689..3979)
                     /locus_tag="PKV97_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HNB05111.1"
                     /translation="MRHDLKVPFAEKDQAKKLGARWDAARKRWYIDGGLDPAVFARWQ
                     PMPHDAAASRRGAPAGAEGKVQVGSRFVALPRVCDCLPWDDCDVCRLQAWPQ"
     gene            complement(4102..4195)
                     /locus_tag="PKV97_04210"
     tRNA            complement(4102..4195)
                     /locus_tag="PKV97_04210"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4159..4161),aa:Ser,seq:gct)
     gene            complement(4256..5488)
                     /locus_tag="PKV97_04215"
     CDS             complement(4256..5488)
                     /locus_tag="PKV97_04215"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNB05112.1"
                     /translation="MALIVQKYGGTSVGTPERMRSVARRVANFRAQGHRVVVVVSAMS
                     GETNRLIALARAVAPSPRTRELDVVVSTGEQVSIGLLCMALDDLGVPARSYTGAQVRI
                     LTDSTHTRARILDIDAAPIRKDLDAGGVVVVAGFQGVDAHGNITTLGRGGSDTTAVAL
                     AAALEADECQIYTDVDGVYTADPRIVPEARKLDAVRFEEMLELASLGSKVLQIRAVEF
                     AAKYKVKLRVLSSFEEDPGEGTFITVEESSNMERPVISGIAFVRDEAKLTLTGVPDRP
                     GIAYQILGPIADADIDVDMIIQNAPRDGSTDLSFTVARADLARAVKVLEGVQAHVGAR
                     SIESDDTMCKVSVVGVGMRSHPGVAAKMFRTLAEEGINIQMITTSEIRISVAIDEKHL
                     ERAVRALHKAYGLDVAAE"
     gene            5743..6036
                     /locus_tag="PKV97_04220"
     CDS             5743..6036
                     /locus_tag="PKV97_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB05113.1"
                     /translation="MKANEKLDVREIRRKLGLNQSQFWSKIGVTQSGGSRYESGRNIP
                     RPVQALLRLVHVEQIDINKVKRDDVEVAEYLKATNPELYKTLKKEARAKRKER"
     gene            complement(6054..6932)
                     /locus_tag="PKV97_04225"
     CDS             complement(6054..6932)
                     /locus_tag="PKV97_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB05114.1"
                     /translation="MNSSQGQPPGTLATLTVGKHDRDLAGLTYVYPVLSRRAGGVSVG
                     INLNPNNACNWHCAYCQVPDLVRGKAPEIDLARLESELRGFLADLIHGSWMERHVPEG
                     ARVIRDIAFSGNGEATSAEVFPEAVALVLRLRDEFGLSPQAVPVRLITNGSLLAQARV
                     QEGVRRLGEAGGEVWFKVDGGSVEAIERINGVRLEPAAVARNLTRCAELCPTWVQTCM
                     LRWDGALPSDADIDAWLAVLEAAGTERLRGVLLYGVARPSMQPEAPRIATLAAEELEA
                     IAERARKKGLTVRVSP"
     gene            7020..7463
                     /gene="queD"
                     /locus_tag="PKV97_04230"
     CDS             7020..7463
                     /gene="queD"
                     /locus_tag="PKV97_04230"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNB05115.1"
                     /translation="MRITRRLEFDAGHRIPDHASQCRHLHGHRYALEVTLSGDIIKAD
                     GVPVNGMVMDFADVKRIANEYVVGPWDHAFLAYRGDHLVLDLLAAIPGHKTVVLDVVP
                     TAENLAAEAFRILDPAYRDTYGNHLRLERVRLYETPNCWADALRD"
     gene            complement(7490..8659)
                     /locus_tag="PKV97_04235"
     CDS             complement(7490..8659)
                     /locus_tag="PKV97_04235"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNB05116.1"
                     /translation="MYNGLFDLPWWGYVLVALALTHVTIVSVTVFLHRHQAHRALELH
                     ALPSHFFRFWLWLTTGMVTKEWAAIHRKHHAKCETAEDPHSPQTRGLHKVLWEGAELY
                     RAEAKNVETLQRYGHGTPDDWLERKVYRHSILGVAIMLVIDLVAFGPLGLTIWAVQMA
                     WIPFWAAGVVNGVGHFWGYRNYDCDDASRNLVPWGILIGGEELHNNHHSFATSAKLSA
                     KWYEFDIGWMYIRLLELLGLAKVKKTIPRPRFGEVKPVPDLDTLQAIVTHRYDVMTRY
                     VRSLREVFVDEARRLGEVHGLELSARRLRRWGLGGELRGLDDTQRRRLEQAVEGSPAL
                     RTVAAMRDELAALWSRSNASREQLLAQLQDWVARAERSGIRPLQEFSLRLRSYAA"
     gene            complement(8809..9363)
                     /locus_tag="PKV97_04240"
     CDS             complement(8809..9363)
                     /locus_tag="PKV97_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HNB05117.1"
                     /translation="MLGWMAALALLALPGVHASERFAGKGEIELAFAPSDDTEKVVIN
                     LIRGARKSLEVHAYAFTSRNIADAMVAAQRRGVRVEVLADAQMNRREKGNAIPRLLAA
                     DVPVAFETRYNAAHNKVLIVDAEGPRCAVLTGSYNFTWSADNRNAENILIVRDHCELA
                     RAYRANWLRHRKDATRVTSLPWRE"
     gene            complement(9413..10756)
                     /locus_tag="PKV97_04245"
     CDS             complement(9413..10756)
                     /locus_tag="PKV97_04245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNB05118.1"
                     /translation="MKPKTEKPRTRAIKRQEPAAAERPAEGAVSRTLFIQATQALGAV
                     LAFDYPADAVLSRHFRDNRELGHRDRGFIAEAVYGVLRRLRWLRRIAGAEATPRMLLL
                     AWFARGEGWPMRNFEGLASATERDWITSIKAAEAGEPSLAERADLPDWLAERLLAQMD
                     GDELLALAHGLNRPAPLDLRVNLIKSDRDALLERLQADGVRAEPGRLSPQAIRLAGKP
                     ALQKHPLFLDGSFEVQDEGSQLLGLLVQPRRGELVVDFCAGAGGKTLQLGAMMRSTGR
                     LYAFDVSEKRLAKLKPRMARAGLSNVHPVLIAHEADAKVKRLAGKADRVLVDAPCTGL
                     GTLRRNPDLKWRQSPAAVEEMVAKQGAILAAAARVVRPGGRLVYATCSLLAEENDGIV
                     DAFLAAHPEFRALSAQEVLAAQGVALETGERLRLLPHVHDTDGFFAAVMERAKGG"
     gene            complement(10758..11495)
                     /locus_tag="PKV97_04250"
     CDS             complement(10758..11495)
                     /locus_tag="PKV97_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNB05119.1"
                     /translation="MRGLVVRLALAACASVAALPVGAAAEPPAQGHIVYDVHYGSSGF
                     KVGEARHEWKLDKGRYALSLALQAKGLAGLFGLEYEQRSSGTVDAKGMRPELFEVDQR
                     GRKPERAEFDWTAGKVSIRRGGEERRNGEIRAGDQDLLSLWHQARKVVESGKPARFAV
                     ITNKSIKQAVVEPRGEERLELAGEQLDTVRLHAFAEKDDLDIDIWLSRRHSLLPVRIR
                     ITDDKGGVLDQRAARIEAGVPAARNPN"
     gene            complement(11495..12151)
                     /gene="purN"
                     /locus_tag="PKV97_04255"
     CDS             complement(11495..12151)
                     /gene="purN"
                     /locus_tag="PKV97_04255"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNB05120.1"
                     /translation="MKSIVILISGRGSNMEAIVRAGIPGARIAAVISNRPGAGGLEFA
                     RAHGIATAVVDHKSHPDRAGFDQALAECIDAHAPDLVVLAGFMRVLGDGFVRRYEGRL
                     LNIHPSLLPAFPGLHTHRRALETGVKVHGASVHFVTAELDDGPIVIQAAVPVLTGDDE
                     DKLAARVLAQEHLIYPQAVRWFVEDRLELVAGRVSLRDAPAQDGEALLVPAPEGGGRA
                     "
     gene            12366..>13449
                     /gene="mutL"
                     /locus_tag="PKV97_04260"
     CDS             12366..>13449
                     /gene="mutL"
                     /locus_tag="PKV97_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNB05121.1"
                     /translation="MPHIHRLSDLLVNQIAAGEVVERPASVLKEVLENAVDAGARAIE
                     VQLEQGGVRRIRVADDGCGIARDELALALERHATSKIATLDDLERVGTMGFRGEALAA
                     IAAVARTGITSRAEGASHAWRIEAGREPEPAALDQGTVVDVADLYYNTPARRKFLKTE
                     STEFAHCDDMFRRVALARPDIGLQLAHNGRVIHRLPPSPPAARVAALMGDDFLQHARE
                     VQADAGILRLAGFASLPAYSRASRDAQYFFVNGRFVRDKLLAHAVREAYADILHGARH
                     PAYVLFLELDPAGVDVNVHPAKIEVRFRESRAVHQFVFHAVRRTLAESGAGRAAELHA
                     PAAIGVGAGPAASPASGTVPQGGFSSA"
BASE COUNT         2017 a         4867 c         4566 g         1999 t
ORIGIN      
        1 ggtgcaggag ccggacgagg ccctgcgctg gctcgccccc cacctgcccg cgcttctgcg
       61 cagcgtcgaa ctgtgcgcgc tgccggccct gccggcgcgc gcgctgcccg agcccgaccg
      121 ccgcttcgat gccggcgtgc ccggacgcaa gcgagcgcag atcgaagctt ttgccgcgcg
      181 tctgcccgcc accggcctgc cactgctcga gtggtgtgca ggcaagggcc acctcggccg
      241 ccgcctggcg ctcgccgacg gcgtgccggt gcgttcgctg gagatcgacc cggccctgtg
      301 cgcggatgcg gagcggctcg ccgagcgcag ccgcttgccg cagcgcgtgg tgtgcgcgga
      361 cgcgctcgat cccgcctccc gccctcacct gcacggccat gcggtgctcg cactgcacgc
      421 ctgcggggag ctgcatcgca gcctggtacg cagcgccgac cgcgacgacg cggccgccta
      481 cgccatcgcc ccgtgctgct accaccgcgg cgccaacggc ggctatcggg cactcggacg
      541 cgacgccagc ctcgagctcg atgccgcaac cctgcgcctg gcggtgaccg agaccgtgac
      601 cgcgccgcgc cacgaccgcc gccgcctcgc ccgcgaccag gcctggaagc tcggcttcat
      661 cgcgctgcgc cagaccgccg agggcggccc cgtgccccac ttccgcccgg tgccgccagc
      721 ctggctgaac ggcagcttcg aggacttctg ccgccgcctc gccgagcgcg agggcctgcg
      781 cctgccgcct gcgctggact gggcggaatg ggaggccgtc ggcgagcgcc ggcgcgacga
      841 ggtgcgccgc ctggagctgg tgcgtcatgc cttccggcgc gcgctcgagg tgtggctcgc
      901 gctcgacctc gtgctcggct tcgaggcgcg cggcttcacg gcgaggatcg gcaccttctg
      961 cgagcgcgcg ctgacgccgc gcaacctgct gatcctcgcc agccggcgtg gcgaggggcc
     1021 gcttcagcca tagcgccgcg ccgcctcgac cgcgaggccg gcgccgatgc tgccgtacag
     1081 gtcgccctcg acgcatcgcg cccgcggcag ctcggccgcg atgcgcgcgc gcagctgcgg
     1141 cacgccgctc gcaccgccgg tgaaatagag cgtgtcgatg cgctcccggt cgacaccgga
     1201 ggccgcgagc aggccggcca cgcagccgcc gacgcgctcg accagcccct gcgtcgccgc
     1261 ggtaaaggcg gcgcgcgaaa gctcgagcac cagcccgggc tcgatgcggt cgagcaccac
     1321 ctgcgtgcgc tccgccgcgg acagcgcgat cttggcctcc tcgacctgca tcgcgagcca
     1381 atgcccctcg cgcaggttga tcagccggca caggcgatcg agctcggtac gcgcgagcgc
     1441 atcgcggtag acctgctgca ggtcggccca gacctgacgg gtgtaggcga aattgatcgt
     1501 gtgccaggcg gcgagctgga aatacacgct cgccggcatc gtggccccgt tgcgcaggcg
     1561 gctgcggtag ccgagcaccg gcatcacgca gtccaggctc aggacgcggt cgaaatcggt
     1621 gccgccgatg tggatgcccg cgttgccgag cagatcctcg cggcgctcga cggcgcgggc
     1681 gcgctgcggc gacaggcgga cgatggagaa gtcggccgtg ccaccgccga tgtcggcgat
     1741 cagcaccagc tcctcgcggc tcagggtgcg ctcgtagtgc agcgcggcgc cgatcggctc
     1801 gaactggaag ccgatctcgc gaaagcccac ctggcgcgcg atggcctcga gcgtgtcctg
     1861 ggccttgcgg tcggcggtct cgtcctcatc gacgaagtgc accgggcggc cgaacaccgc
     1921 atggtcgaag gtgcgccccg tcgcacgctc ggcgcgtgcc ttgagttcgc cgatgaagct
     1981 cgcgagcagc tcgcggaagc cgagcgcgcg gccattcacc tcggtctgcc cgtcgatcag
     2041 gctggtgccg agcaggctct tcagcgcgcg catcagccgc ccttcgtagc cctccaggta
     2101 ttcggcgagc gccgcacggc cgaactggat cgaatcctcg tcggcgttga agaacaccgc
     2161 cgacggcagc gtcgcgcggc cgtcctcgag cgggacgagg atgggcgcgt cggggcgcag
     2221 ccagccgacg gtggagttgg aagtgccgaa gtcgatgccg caggcgcggg gaggctgggt
     2281 catggggtcg ctcgcggaac gggatgcact cggcgcgcac aaacggaaag gtcgcgatta
     2341 tgcgtcccgc gcggcccgac tgcgagcgct cgcgcggcac gatctcgcag gcaccggcca
     2401 cgacgatcgc ccgccctcgc cgtgcttcgg gctaacatcg ccccgatcca tcacacgagc
     2461 acggcaccat gaactccaag gacaccacgc cccccgccag caccttctcc accccgatcg
     2521 acgaccgcta tttcgaggac tacgtccccg gcgacgtgca tcgcttcggt gagatgcgcg
     2581 tcaccgagca ggagatcatc gagttcgcgc gccgctacga cccgcaggat ttccacaccg
     2641 accccgcgaa ggccgcggac acccagttcg gcggcctgat cgccagcggc tggatgacct
     2701 gcgggatgat gatgcggatg tactcggagc attacctcac ccacaacgcc agcctggcct
     2761 cgcccggcat cgacgagctg cgctggctcg agccggtgcg tccggacgac gtgctgagca
     2821 cgcgggtcac catcgtcgag gcccggcgtt cggcctccaa gcccgaccgt ggcctggtgc
     2881 gctcgaagat cgaggtgctc aaccagcacg acagggtggt cctgagcatg ctcgcgatga
     2941 atctgatcgg ctgccgcacc cccgccgcgg cctgagctgc gcggacacag cgcggcggat
     3001 gcggacaccg atgggagcgg gcgagccccg cgcccggccg ctcacccgtg cctgttcgct
     3061 cacccttcac gtgcagccgc caggcacttg ggcagttgcg ccttcgaggc gcgctggatg
     3121 cgacacagca gctccaccga cacctgcgcg acccggtggc gctggcgcag ctcctcttcc
     3181 aggcgcagca gcaggctggc cgccatctcg gcgtcggcga gcgcacggtg gaagcggccg
     3241 gccaccggca gcctggcgta ctccaccaac gcgccgagcc tgtggctggg ggcaagcgga
     3301 aacaggcggc gcgacagcag cagcgaacac acgaagtcct gcttgcgcgc gcgctcgatg
     3361 cgagcgagct cggcatccca gaacttcgca tcgaatgcgg cgttatgggc gaccatgggg
     3421 atgccgccga tgaagtccgc gacctcgcgc atcacctgcg ccgcagtcgg ggcgctgcgg
     3481 atcatcgcgt tggagatccc ggtcagggat tcgatatggg ccgggatgcg cacgcccgcg
     3541 ttcatcaggc tctggtagcg atccaccacc cggccgtccc gcacgatcac cgcggcgatc
     3601 tcggtcgcgc ggtcgccctg cgcaggcgac agccccgtgg tctcgaagtc gatcaccgcc
     3661 accgcttcca tgcctgcacc ctccccgtct attgcggcca cgcctgcagg cggcagacgt
     3721 cgcagtcatc ccagggcagg cagtcgcaca cccgcggcaa cgcgacgaag cggctgccga
     3781 cctgcacctt gccctcggca ccggcaggcg cgcctcgcct cgacgccgcc gcgtcgtgcg
     3841 gcatcggctg ccacctcgcg aacacggccg gatcgaggcc gccgtcgatg taccagcgct
     3901 tgcgcgcagc gtcccagcgc gcaccgagct ttttcgcctg gtccttttcg gcgaagggca
     3961 ccttcaggtc gtgtcgcatg ggggtcaccg ggcgggaagt tggaagtccg gcacaggccg
     4021 ccgcgggatg cctcgggcgc atccgctctg cgcactgtgg acgcaaaaaa gcccctttat
     4081 ggatcagggg cttgagtgct ctggcggaga cggagggatt cgaaccctcg atgcgagttt
     4141 tagcccgcat gctcccttag caggggagtg ccttcgacct ctcggccacg tctccatcag
     4201 gaaggcgcga tcatagcgag ttcgcgcctg ccggtcaaac gttgatcgaa cgcatctatt
     4261 cagcggccac atccaggcca taggccttgt gcagggcgcg caccgcgcgc tcgaggtgct
     4321 tctcgtcgat cgccaccgag atgcggatct cggaggtcgt gatcatctgg atgttgatgc
     4381 cctcctcggc cagggtgcgg aacatcttcg cggcgacgcc ggggtgcgag cgcatgccga
     4441 cccccaccac cgagaccttg cacatggtgt cgtcgctctc gatcgagcgc gcgccgacgt
     4501 gggcctgcac gccttcgagc accttcacgg cacgcgcaag atcggcgcgc gcgacggtga
     4561 aggagaggtc ggtcgagccg tcgcgggggg cgttctggat gatcatgtcc acgtcgatgt
     4621 cggcatcggc gatcggaccg aggatctggt aggcgatacc cggccggtcg ggcacgccgg
     4681 tgagggtgag cttggcctcg tcgcgcacga aggcgatgcc ggagatgacc ggtctttcca
     4741 tgttgctgct ttcctcgacg gtgatgaacg tgccctctcc cgggtcttcc tcgaaactgg
     4801 agagaacccg cagcttgacc ttgtacttgg cggcgaactc gaccgcgcgg atctgcagca
     4861 ccttcgagcc caggctggcg agctccagca tctcctcgaa gcggacggca tcgagcttgc
     4921 gcgcctcggg gacgatgcgc ggatcggcgg tgtaaacgcc gtccacgtcg gtgtagatct
     4981 ggcattcgtc cgcctcgagc gcggcggcca acgccacggc agtggtatcc gagccgccac
     5041 ggccgagggt tgtgatgttg ccgtgtgcgt ccacgccctg gaagccggcg accaccacca
     5101 ccccgcccgc gtcgaggtcc ttgcggatcg gcgccgcgtc gatgtcgagg atgcgcgccc
     5161 gggtgtgcgt cgagtcggtg agaatcctta cctgcgcgcc agtgtagctg cgtgccggga
     5221 cgccgaggtc gtcgagcgcc atgcacagca agccgatgct gacctgctcc ccggtggata
     5281 cgacgacgtc gagctcgcgc gtgcggggac ttggcgcgac tgcgcgggcg agcgcgatca
     5341 ggcggttggt ctcgccgctc atcgccgaca cgacgacgac cacgcgatgg ccctgcgccc
     5401 ggaagttcgc caccctgcgg gcgacgctcc gcatgcgttc gggggtcccc accgaggttc
     5461 caccgtactt ctgaactatc agcgccatcg tcgtatccaa aaccacaggt gaataaggtc
     5521 cgcgatgcta tccggcggcg tgaagttttg ctcgcccgat cgaaacaaaa tctccaggac
     5581 gtgttttttc ttacttattt gctgaatttg ctaaaatcta ttttgtatgt agagagaata
     5641 ctaacctata ctgcatgcgt tctatgacct tagtcatagg cagaatcctg tccgcatcga
     5701 gtccgggaag gcccgaacgg atacaaactc gaggaaatct ccatgaaagc aaatgaaaag
     5761 ctggatgtac gcgaaattcg ccgcaagctc ggcctgaacc agtcgcagtt ctggtccaag
     5821 atcggcgtga cccagagcgg tggctcgcgc tacgagagcg gccgcaacat tccccgcccg
     5881 gtgcaggcct tgctgcgcct ggtccatgtc gagcagatcg acatcaacaa ggtcaagcgc
     5941 gacgacgtcg aggttgccga gtacctcaag gcgaccaacc ccgagctcta caagaccctc
     6001 aagaaggaag cacgcgccaa gcgcaaggag cgctgagcgc atgccgcgcg cgatcaaggg
     6061 ctgacgcgca ccgtcagccc tttttttcgg gcgcgctcgg cgatcgcctc gagttcttcc
     6121 gccgccagcg tcgcgatccg cggcgcctcc ggctgcatcg acggccgcgc cacaccgtag
     6181 agcagcacgc cgcgcaggcg ctccgtcccg gcggcttcca gcaccgccag ccaggcgtcg
     6241 atgtccgcgt ccgacggcag cgcgccgtcc cagcgcaaca tgcaggtctg cacccaggtc
     6301 gggcacagct cggcgcagcg cgtcaggttg cgcgccaccg cggcgggttc cagccgcacc
     6361 ccgttgatgc gctcgatcgc ctcgacgctg ccgccatcca ccttgaacca cacttcgccg
     6421 cccgcctcgc ccaaccggcg cacgccctct tgcacgcgcg cctgcgcgag caggctgccg
     6481 ttggtgatca ggcgcaccgg caccgcctgc ggtgacagcc cgaactcgtc gcgcagccgc
     6541 agcaccagcg cgaccgcctc ggggaagacc tcggcgctgg tcgcctcgcc gttgccggaa
     6601 aaggcgatgt cgcggatcac ccgcgcgccc tcgggcacgt ggcgttccat ccagctgccg
     6661 tgaatgaggt cggcgaggaa gccgcgcagc tcgctctcga gccgggcgag gtcgatctcc
     6721 ggcgccttgc cgcgcaccag gtcgggcacc tgacagtagg cgcaatgcca gttgcaggcg
     6781 ttgttcgggt tcaggttgat gccgaccgac accccgccgg cgcggcgcga cagcaccgga
     6841 tagacgtagg tcagcccggc gaggtcgcga tcgtgcttgc ccaccgtcag ggtcgcgagc
     6901 gtgccggggg gctgtccttg ggaagaattc atcgtttgtt caaccacttg cggaaaatac
     6961 gcggagtcgg cgcgggcacg ccggtgctat actccggagc ctttccagag agacccgcca
     7021 tgcgcatcac ccgccgcctc gaattcgacg ccggacaccg gattccggac cacgccagcc
     7081 agtgccggca tctgcacggc caccgctacg cgctggaggt gacgctgtcg ggcgacatca
     7141 tcaaggccga cggcgtaccg gtcaacggca tggtgatgga cttcgccgac gtcaagcgca
     7201 tcgccaacga atacgtcgtc ggcccctggg accacgcctt cctggcctac cgcggcgacc
     7261 acctggtgct cgacctgctg gccgcgatcc ccggccacaa gaccgtcgtg ctcgacgtcg
     7321 tgcccaccgc cgagaacctc gccgcagagg ccttccgcat cctcgacccg gcctatcgcg
     7381 acacctacgg caaccacctc aggctggagc gcgtgcgcct gtacgagacc cccaactgct
     7441 gggccgacgc cctgcgcgac tgagtcgcgc ctgacgcccc acccctcgct cacgccgcat
     7501 agctgcgtag ccgcagcgaa aactcctgca gcggacggat gccgctgcgc tcggcgcgcg
     7561 ccacccagtc ctgcagctgc gcgagcagct gctcgcggct ggcgttcgag cgcgaccaca
     7621 acgcagcgag ctcgtcgcgc atcgccgcca ccgtgcgcag cgccgggctg ccctcgacgg
     7681 cctgctccag ccgcctgcgc tgggtgtcgt ccagcccgcg cagctcgcca cccagccccc
     7741 agcgccgcag ccgacgcgcg ctcagttcca gcccgtgcac ctcgcccagc cggcgcgcct
     7801 cgtcgacgaa gacctcgcgc agcgaacgca cgtagcgcgt catcacgtcg tagcggtgcg
     7861 tcacgatcgc ctgcagggtg tcgaggtcgg gcaccggctt gacctcgccg aacctgggcc
     7921 ggggaatcgt cttcttgacc ttggcgaggc cgagcagctc gagcaggcgg atgtacatcc
     7981 agccgatgtc gaactcgtac cacttcgccg acagcttggc ggaggtcgcg aagctgtggt
     8041 ggttgttgtg cagctcctcg ccgccgatca ggatgcccca gggcacgagg ttgcgcgagg
     8101 cgtcgtcgca gtcgtagttg cggtagcccc agaagtgccc cacgccattg accacccccg
     8161 ccgcccagaa cgggatccag gccatctgta ccgcccagat cgtcagcccg agcgggccga
     8221 aggcgacgag gtcgatgacc agcatgatgg ccacgccgag gatggagtgg cggtagacct
     8281 tgcgttcgag ccagtcgtcg ggcgtgccgt ggccgtagcg ctgcagcgtc tcgacattct
     8341 tcgcctcggc gcggtagagc tccgccccct cccacagcac cttgtgcagg ccgcgcgtct
     8401 gcgggctgtg cgggtcctcc gcggtctcgc acttcgcgtg gtgcttgcgg tggatcgccg
     8461 cccactcctt ggtgaccatg ccggtggtca gccatagcca gaagcggaag aaatggctgg
     8521 gcagcgcatg cagctcgagc gcgcggtgcg cctgatggcg atgcaggaag acggtgacgg
     8581 agacgatggt gacgtgggtg agcgcgagcg ccaccagcac gtagccccac caggggagat
     8641 cgaagagacc gttgtacatg cgaggaaacg tccacaagag aaaacagcag gacgattcta
     8701 cgcaaggccg ttccggattg gatgcagtcc agcctttgtt accccgccat accgcgggag
     8761 gggtcttccg gtgcccgcat cgcgacctgc gtcgctgcta cgagaacctt actcccgcca
     8821 cggcagggag gtgacgcggg tcgcgtcctt gcggtggcgc agccagttgg cgcggtaggc
     8881 gcgggcaagc tcgcagtggt cgcgcacgat gaggatgttc tcggcgttgc ggttgtcggc
     8941 cgaccaggtg aagttgtagg agccggtcag caccgcgcag cgcgggcctt cggcgtccac
     9001 gatcagcacc ttgttgtgcg cggcgttgta gcgcgtctcg aaggccaccg gcacgtcggc
     9061 ggcgagcagg cgcgggatcg cgttgccctt ctcgcggcga ttcatctgcg cgtcggcgag
     9121 cacctcgacg cgcaccccgc gccgctgcgc cgccaccatc gcgtcggcga tgttgcggct
     9181 ggtgaaggcg taggcgtgca cctccaggct cttgcgcgca ccgcggatca ggttgatcac
     9241 caccttctcg gtgtcgtcgc tcggagcgaa ggcaagttcg atctcgccct tgcccgcgaa
     9301 acgctcgctc gcgtgtacgc cgggaagcgc caggagggcg agcgccgcca tccacccgag
     9361 catcggcgcg cggcgcttgc ctgcccaccg atgcgcccgc tcatgtccgc gctcacccac
     9421 ccttggcacg ctccatgacg gcggcaaaga agccgtcggt gtcgtgcaca tgcggcagca
     9481 ggcgcaggcg ctcgccggtc tccagcgcca caccctgcgc cgccagcact tcctgcgccg
     9541 acagcgcgcg gaactcggga tgcgcggcca ggaaggcgtc gacgatgccg tcgttctcct
     9601 cggcgagcag gctgcaggtg gcatacacca ggcggccgcc cgggcgcacc acgcgcgccg
     9661 ccgcggcgag gatcgcgccc tgcttggcga ccatctcctc gaccgccgcc ggcgactggc
     9721 gccacttgag gtcggggttg cggcgcagcg tgcccagccc ggtgcagggc gcatcgacca
     9781 gcacgcggtc ggccttgccc gccaggcgct tgaccttggc gtcggcctcg tgggcgatca
     9841 gcaccggatg cacgttcgac aggccggcac gcgccatgcg cggcttgagc ttggcgagcc
     9901 gcttctcgga aacgtcgaag gcgtacaggc gcccggtcga gcgcatcatc gcgcccagct
     9961 gcagggtctt gccgccggcg ccggcgcaga agtccaccac cagttcgccg cggcgcggct
    10021 gcacgagcag gccgagcagc tggctaccct cgtcctgcac ctcgaagctg ccatcgagga
    10081 agagtggatg cttctgcagc gccggcttgc ccgccaggcg gatcgcctgc ggcgacagcc
    10141 ggccgggctc ggcacgcacg ccgtcggcct gcaggcgctc gagcagcgcg tcgcggtcgc
    10201 tcttgatcag gttcacccgc aggtcgagcg gcgccgggcg gttgagcccg tgcgcgagcg
    10261 cgagcagctc gtccccgtcc atctgcgcga gcaggcgctc ggccagccag tcgggcaggt
    10321 cggcgcgctc ggccaggctg ggttcgcccg cctcggcggc cttgatcgag gtgatccagt
    10381 cgcgctccgt ggcgctcgcc aggccctcga agttgcgcat cggccagccc tcgccgcgcg
    10441 cgaaccaggc cagcagcagc atgcgcggcg tggcctccgc gccggcgatg cggcgcagcc
    10501 agcgcagccg gcgcagcaca ccgtacaccg cctcggcgat gaagccgcgg tcgcggtgac
    10561 cgagctcgcg gttgtcgcga aaatggcgcg acagcacggc gtcggccggg tagtcgaagg
    10621 cgagcacggc gccgagcgcc tgcgtggcct ggatgaagag cgtgcgcgac accgcgccct
    10681 cggccgggcg ctcggctgcg gccggctcct gccgcttgat cgcccgggtc cggggctttt
    10741 cggtcttggg tttcatttca gttcgggttc ctggcggcgg gcacgcccgc ctcgatgcgc
    10801 gcagcgcgct ggtcgagcac gccgcccttg tcgtcggtga tgcgaatgcg gaccggcagc
    10861 aggctatggc gcctggacag ccagatgtcg atgtcgaggt cgtccttctc ggcaaaggcg
    10921 tgcaggcgca cggtgtcgag ctgctcgccg gcgagctcga ggcgctcctc gccgcgtggc
    10981 tcgaccaccg cctgcttgat gctcttgttg gtgatcacgg cgaagcgcgc cggcttgccg
    11041 gactcgacca ccttgcgcgc ctggtgccac aggctgagca gatcctggtc gccagcgcgg
    11101 atctcgccgt tgcgccgctc ctcgccgccg cggcggatgc tgaccttgcc cgcagtccag
    11161 tcgaactcgg cgcgctcggg cttgcgcccg cgctggtcga cctcgaacag ttccgggcgc
    11221 atgccctttg cgtccaccgt gccgctgctg cgctgctcgt attcgaggcc gaacaggccg
    11281 gcgagcccct tggcctggag cgcgagcgag agcgcgtagc gccccttgtc cagcttccac
    11341 tcgtggcgcg cctcgcccac cttgaagccg gacgagccat agtgcacgtc atagacgatg
    11401 tggccctgcg cgggcggctc ggccgctgcg ccgacgggca gcgccgccac gctcgcgcag
    11461 gcggcgagcg cgaggcgcac gaccagcccg cgcatcacgc ccgccctccg ccctcgggcg
    11521 caggcaccag cagcgcctcg ccgtcctggg ccggcgcgtc gcgcaggctc acgcggccgg
    11581 cgacgagttc gagccgatcc tcgacgaacc agcgtaccgc ctgcgggtag atgaggtgtt
    11641 cctgcgccag cacgcgcgcg gccagcttgt cctcgtcgtc gcccgtcagc accggcaccg
    11701 cggcctggat cacgatcggc ccgtcgtcga gctcggcggt gacgaaatgc acgctcgcgc
    11761 catgcacctt caccccggtc tcgagcgcgc ggcggtgggt gtgcagcccc gggaaggccg
    11821 gcagcagcga ggggtggatg ttgagcaggc ggccttcgta gcggcgcacg aagccgtcgc
    11881 cgagcacgcg catgaagccg gcgagcacga ccaggtcggg cgcatgggcg tcgatgcact
    11941 cggcgagcgc ctggtcgaag ccggcgcggt cggggtggga cttgtggtcg acgaccgcgg
    12001 tggcgatgcc gtgcgcgcgt gcgaactcga gcccgccggc gcccggccgg ttgctgatca
    12061 ccgcggcgat acgcgcgccc gggatgcccg cacgcacgat ggcttccata ttgctgccgc
    12121 ggccggaaat gaggatgacg atggacttca tgggatgctg gaacgatgaa aagatcctgg
    12181 cggaggacag ggcttgggcg cgcggaccgg cgggcgcccg ccccggagca tggccgcgat
    12241 gatacccgac ccgcgccacg ctcccggcac gggcaaggaa aatcgccggt tttctgcaca
    12301 agcggcgaaa ggctgtgccg cccccgcacc cccgtataat cccgcttttg cgtcgccgcc
    12361 cccccatgcc ccacatccac cgcctctccg acctgctggt caaccagatc gccgccggcg
    12421 aggtggtcga gcgcccggcc tcggtgctca aggaagtact cgagaacgcg gtcgatgctg
    12481 gcgcgcgcgc gatcgaggtg cagctcgagc agggcggcgt gcggcgcatc cgcgtcgccg
    12541 acgacggctg cggcatcgcg cgcgacgagc tcgcgctcgc gctcgagcgc cacgccacca
    12601 gcaagatcgc caccctcgac gacctcgagc gcgtgggcac gatggggttt cgcggcgagg
    12661 cgctggcggc catcgccgcg gtggcgcgca ccggcatcac cagccgcgcc gagggcgcga
    12721 gccacgcctg gcgcatcgag gccgggcgcg agcccgagcc ggcggcgctc gaccagggca
    12781 cagtggtcga cgtcgccgac ctctactaca acacccccgc gcggcggaag ttcctcaaga
    12841 ccgaatccac cgagttcgcc cactgcgacg acatgttccg ccgcgtcgcg ctcgcgcgcc
    12901 ccgacatcgg cctgcagctc gcccacaacg gccgcgtgat ccaccgcctc cctccctcac
    12961 cgcccgccgc gcgggtggcg gcgctgatgg gcgacgactt cctgcagcac gcccgcgagg
    13021 tccaggccga cgccgggata ctgcgcctgg ccggcttcgc gtcgctgccc gcctattcgc
    13081 gcgccagccg cgacgcgcag tatttcttcg tcaatggccg cttcgtgcgc gacaagctgc
    13141 tcgcccacgc ggtgcgcgaa gcctacgccg acatcctgca cggcgcgcgc catccggctt
    13201 acgtgctctt cctcgagctc gaccccgccg gggtggatgt gaacgtgcat ccggccaaga
    13261 tcgaggtgcg cttccgcgag tcgcgcgcgg tgcatcagtt cgtcttccac gccgtgcgcc
    13321 gcaccctggc cgagagcgga gcgggacgcg ctgcggagct gcacgcgccc gcagccattg
    13381 gcgtcggtgc cggccccgcg gcctccccgg catcgggcac cgtcccgcaa gggggcttct
    13441 cgagcgccc
//
LOCUS       DAOQNN010000040        13100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2012_length_13100_cov_5.939440, whole genome shotgun sequence.
ACCESSION   DAOQNN010000040 DAOQNN010000000
VERSION     DAOQNN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 13100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13100
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2012_length_13100_cov_5.939440"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PKV97_04265"
     CDS             <1..903
                     /locus_tag="PKV97_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05122.1"
                     /translation="KPHMQRMNATLANPLLPPPVRIALALMAAGAMSACAPSGLEARR
                     TEARSLAAHAGWERVAIDSGRFVLIAHRPHPVQRVDTLSVYIEGDGLAWLSRSEPSRD
                     PTPLDSVALGLALRQPSGAAVHLARPCQFTLDPERRNCSKQYWTSHRFAGEVVDSMSR
                     AIDRLKADQGAQGLELVGYSGGGAIAALLAARRGDVERLITVAGNLDPAAWTRLHRIT
                     PLEGSLDPVDVAPALSKLEQIHLAGAKDRELPAELAEGWLRRLRTPSAARLQVIPDFD
                     HHCCWVEAWPTLYPDPAPRPDPAR"
     gene            complement(864..1736)
                     /locus_tag="PKV97_04270"
     CDS             complement(864..1736)
                     /locus_tag="PKV97_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05123.1"
                     /translation="MDELIRALGGLDRVVGVGIGGSRGLRIASEASDFDLVLFRRGGE
                     RVDAREILDALRACAGVCELRDPAGFVSGRLGTHRFEIFQKDLLAIEREIEQAREGAF
                     TWSIRQLFPHGDLSTGLISHVVHLIVCSDPDGHLERLRAAATPFPVALRAALVDTFLQ
                     QADITVTHAAKISRRLDLQYLVGLCSGFVFFANLLVFAVNGVYPVLEKGGSLVIARLP
                     QRPAHYEQRIAQLFSCAAAGDYPAAVAMLETLLGELRFLAVEGRRALAADESVRTRPN
                     EAQRAGSGRGAGSG"
     gene            complement(1772..2287)
                     /locus_tag="PKV97_04275"
     CDS             complement(1772..2287)
                     /locus_tag="PKV97_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HNB05124.1"
                     /translation="MLKARAPRAIPLLLSLSLLLAPSMLRAQVAQGERFGDWVYECQA
                     LSGASKACALVQVHSFGEGDKRSVRFNISRPPQGGGVGMSVVVPLGIHLPNGIRGDVD
                     GKNGFAMTPLTCVAQGCIATVPLDAGMIEALKAGRVLNLEFYGNAGRQQVKVAGSLKG
                     ITSGLRASGLD"
     gene            complement(2432..3919)
                     /locus_tag="PKV97_04280"
     CDS             complement(2432..3919)
                     /locus_tag="PKV97_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNB05125.1"
                     /translation="MDALSGLMAGFEVALSPGNLWFCLIGVALGTLVGVLPGVSSLAA
                     VAMLLPFTHALSPTAALVLLAGVYYGSEYGGAIAAILLNVPGTPASAVSCLDGHPMAR
                     AGRAREALILSAGASFGGAVVGIGMMLAMAAPLAGLAFMLGPAEYFAIMIFGLVCTAA
                     VGRGRLAAGMVAMLAGVGLAMVGTDAQTGVARFTLGLTELRDGIPLVVLAMGLFGVSE
                     VMLSMFGALPVVRGGIGERSALPRWKAWRRAAGPVLRGGVLGGLFGALPGTGPTLASI
                     AAYALERRLSARPECFGRGAIAGLAAPEAANNAAAQTAFVPTLALGIPGSATMALMLG
                     AMSAHGVVPSPLLAVENPELFWGLMASFVIGNLLLLVLNVPLVGVWVRILRLPPQRLY
                     PLVLVLIAVATFGVRGSAFDVWAALAIGLVAYGMRRSGVHLAPVLIGFVLGPLVEDNF
                     RRALAISQGDFAIFVSSPIALAALGAVFVLLAVAVCRSMRSARAA"
     gene            complement(3907..4434)
                     /locus_tag="PKV97_04285"
     CDS             complement(3907..4434)
                     /locus_tag="PKV97_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HNB05126.1"
                     /translation="MTPTQDRRVRGQGPDRQARLWTLVLLALSGLGAWLVLGGAALGL
                     GSLRRLGPGAFPALAGAALLIAAALACLRPWSMSADDSSTPREGDDAGLRGVWAAVAG
                     LAGFASLAPVLGYVPAAFAAALLASAGDARWERRGKLLQAVVLAGLVAAMFGVGLELP
                     AASFRNPFAGVSWMP"
     gene            4523..5500
                     /locus_tag="PKV97_04290"
     CDS             4523..5500
                     /locus_tag="PKV97_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNB05127.1"
                     /translation="MNRRHFIGALAGLAAARARAGEGNGVRMIPERPTLLVAVAAGGG
                     PDSVARAIAGHLADRHGIHARIVNLPDANGERALADFLTLPADGSTWLIAADSLIVVN
                     PRLYPRESADPLHGLAPVAQVGSNFFFLAVGAEDPIRSMAELVLEARAAERPLNYASG
                     GIGSMHHLLMADLAARLGLRLNHVPYKGGGAAATGLARGDVRVAFAGASALPLVRAGK
                     LRLLAVAAPQRSPRFPDLPSLAEVAPGFDGTLWFGWYGHAGTPAPIRDAMRGLLQEAM
                     ASPQTSAALDRQHGGISPSFTSGPAFDALIERERERFARLISLLPEQTR"
     gene            5689..6411
                     /locus_tag="PKV97_04295"
     CDS             5689..6411
                     /locus_tag="PKV97_04295"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB05128.1"
                     /translation="MSGFSAEFVAAIRQAAQTHRGPSFRILISGCADYSAYAHVLAGC
                     AGLSVQPRVTALDLCATPLILSRWYARRHGGDLAAVCSDILDHRHPGGYELILTSSFL
                     GYFPPARRAELFSRYAALLAPGGRLIFTTRLRPGAEDQLAPFGPDQKEALVRTVVDSL
                     TELSGLGLDRREAAQLAAAYADLCASYPVNGEASLRRLAEAAGLLWLDCDRRRSEAAR
                     PGVTGPTVGAAEYLFVTLGRPA"
     gene            complement(6430..8214)
                     /locus_tag="PKV97_04300"
     CDS             complement(6430..8214)
                     /locus_tag="PKV97_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNB05129.1"
                     /translation="MVELVRVGTRFTWLRGAAFGLQQGALAGELVLTDEGRAGIVEEM
                     LAAFADEASTGGERAAGASGVECLIDCILRCVGALQRRAGIPVSWRSHRALIRTVADV
                     RVFRVALPSDQPAATERALRWVVAEVNRRATGGQAGSKDGRSSQESLEELDRMLGAHA
                     IPGINPINIALAAYRAGIPVGRLLHDVLVLGQGRKARWMRSTITDRTSGIGLGIARNK
                     VHTAEVLRAAGLPVPQQVVVRSPEGAVEAARALGYPVVVKPADQEQGAGVQAGLSDDA
                     MVRIAYDAASALSRTILVERHVEGYTHRLTVFQGRVVRVMRRVAGGVVGDGVSAVATL
                     VERRLEDEIHRRRAQRAGAPLLSLDAEALDLLARRGMGPASVPAAGEYIRLRRRDNIN
                     AGGENRPCALGDVHPANLQLAVDAAMALRLDLAGVDLIIEDVSRSWLEAGGAICEVNG
                     QPQMGGNSHAGVLDAVVRELVGGDGRIPVHLVVCPDREDWHAGLSARLLQHGEVDGVS
                     SRHGLVLRGARVSKPFEDALAAARALLCRADVGSAACLMSAPDILRLGLPSDVLDSLR
                     VADETAMSAAERGALAAIRPMIEPHLRR"
     gene            complement(8259..11570)
                     /locus_tag="PKV97_04305"
     CDS             complement(8259..11570)
                     /locus_tag="PKV97_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB05130.1"
                     /translation="MNVVDLLLQQASSRPEALAVRHGDEVLGYGALGRRVCSVAAGLA
                     NAGVARGDVLALCLDDPLDHLVASLAVAHRGATVVSVPRTLPDALRGRLLALTRAGRA
                     LADDGRRTGADLGGLPILAWSMLERSPPSMTPEPAREVADRPWIYVTGSGSTGRPKIM
                     PVTHRQQCLRSRLGPSWLPYGAQDVLCSMVSMHFYSAKQRCLEAMAIGAGIFLDTPGR
                     VDLRREVAEGEVTVIHAMVCHIETLLRRLPAEATGAFARLQALMVGGSTVSMALRDEI
                     RRRLTPRIHVFYGTNESHTATLTRLDEVHRTSGGVGRPFPGIRIEVVDERGQPVPAGV
                     DGRVRIASPMVIDGYLGDEEANRKAFAEGWFYPGDIGHLTTDGQLVHRGRMDDMMIVG
                     GVNLHPAEIEQCLRRHPGVADALATPLRHRVLQDVPVALVVPEAGTHPSAHELVAHVR
                     AALGRHALHDVIWVDRIPRSEQGKVQREAVEAVLERHDWAHEGGRAQGARRHWSRPRQ
                     LQTRFEIEFAPPIAPATNRLDAWMALLGEDAPLGTRPSEGCEDADTARYWLQQVLRLA
                     RLFLQLVRVPVFDVFELIDCAPVDEHRRRWRAAFLCPGPEFVSATACMEVVGIAFRIA
                     QLAQGADPGSPQDRERVFVAIGDELLQPHGKLMPAGKSTLEVLRAAYRLDIPFRPLGR
                     GVYQLGWGSRARRIERSTTDGDSVFGMRWTHDKWMASELLRAAGLPAPVNRLVMTAEE
                     ARAAARSLGYPVVVKPVDRDRGEGVSVDVGDDEGVARAYTTARECSPGKRVLVERQAP
                     GVCHRIFVAAGRLLYAVRRLPAGVYGNGRTSIGSLVDAECARRMGLPPWKRAPLCALD
                     DSALEMLARQGLGPEDVPGAGRFVALRRIETTASGGVDEEVTHALHPENVRAAIAAAR
                     LFGLEVAGVDMISPDITAPWHANGAILNEVNFAPLLGGGEISRRYVEEYLFRLVDGRG
                     RIPVEVFVGGAAAWEAAAARWRTLLQAGMAAVLSSDVRTLGGDGQPFGLAVDGLHARV
                     RALTLRAEVGALVLVVQTLELLRTGIPLDRVDRVVVIDGDIRTAGSPARQAADAEVAG
                     LIALCRAWDASGTAS"
     gene            11751..12821
                     /locus_tag="PKV97_04310"
     CDS             11751..12821
                     /locus_tag="PKV97_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05131.1"
                     /translation="MQIQLLIPGLLWPVATLLGPASGLALEGLATLLGRGRRELTAFE
                     PYDRQLARLFGVRGDTLPMATLRRLGEADAPAPEPGRHWLCADPVNLSFAREHLLLQA
                     FPDEELDAAESAELVAELNAIFADLGRFEACTPTRWYLRLHRPTAVTLYPLDDVTGRP
                     VKHFLPEGEDARLWQRTMNEAQIVLHNHARSRAREEAGRRAVNSVWLWGAGALDAPPR
                     APARLVQASDPVSIGLARATGVAFGAPDPAAALAQDTLVVLDELRKPAQQLDLDTWRR
                     GLEAMERDWFGPLAEAFRAGRIDTLRLTAPGDRGTLQLELRAGERWKFWRKPYAFDAL
                     LKSIAPAPMQMPDAPRPAHGAL"
BASE COUNT         2108 a         5010 c         4227 g         1755 t
ORIGIN      
        1 aaacctcaca tgcaacgcat gaacgcaaca ctcgcgaatc ctctccttcc tccgcccgtg
       61 cgcatcgcac tcgcgctcat ggcagccggc gccatgagcg cgtgcgcgcc gtccgggctc
      121 gaagcgcgcc ggaccgaagc gcggagcctc gccgcgcatg caggctggga gcgtgtcgcg
      181 atcgacagcg gacgcttcgt gctgatcgct caccgccccc atcccgtgca gcgcgtggac
      241 acgctcagcg tctatatcga gggtgacggg ctggcctggc tgagtcgctc ggagccatcg
      301 cgcgacccca cgccgctcga ttcggttgcc ctgggcctcg cgttgcgtca accctcgggg
      361 gccgcggtac atctggcccg accctgccaa ttcaccctcg accccgagcg ccgcaactgc
      421 agcaagcaat actggacctc ccatcgcttt gccggcgagg tcgtggattc gatgagccgc
      481 gcgatcgatc gcctcaaggc ggatcagggt gcgcaagggc tggaactggt ggggtattcg
      541 ggcggggggg cgatcgcggc gctgctcgcc gcgcgccgcg gcgatgtcga acgtctgatc
      601 accgtcgccg gcaacctcga ccctgcggcc tggacacgcc tgcaccggat caccccgctc
      661 gaaggatcgc tggatccggt cgatgtggcg cccgcgctct cgaagctgga gcagatccat
      721 ctcgcggggg cgaaggatcg cgaactgccc gctgagctcg ccgaaggatg gctgcgccgc
      781 ttgcgcaccc ccagcgcggc ccgcctgcaa gtgattcccg acttcgacca tcactgctgc
      841 tgggtcgagg cctggccgac gctctacccg gatccggcac cccgccccga cccggcgcgc
      901 tgagcctcgt tcggtcgcgt gcgcaccgac tcgtcggcgg cgagcgctcg acggccctcg
      961 acggccagga accggagttc cccgagcagg gtctcgagca tcgccaccgc cgccgggtag
     1021 tcccccgcag ccgcgcaaga aaagagctgc gcgatccgct gctcgtagtg cgccggacgc
     1081 tgcggcaggc gggcgatgac caggctgccc cccttctcca gaaccggata caccccattc
     1141 accgcaaaga cgaggaggtt ggcgaagaag acgaagcccg agcacagccc gacgaggtat
     1201 tgaagatcca gccgacggga gatcttcgcc gcatgcgtga cggtgatgtc ggcctgctgg
     1261 aggaaggtat cgacgagcgc ggccctgagc gcgaccggga aaggcgtggc ggcagcgcgc
     1321 aggcgctcca ggtgtccgtc ggggtcgctg cacacgatca gatggacgac atggctgatc
     1381 agcccggtgc tcaagtcgcc atgcggaaag agctgccgga tcgaccaggt gaatgcgccc
     1441 tcccgcgcct gctcgatctc gcgctcgatg gccaggagat ccttctggaa gatctcgaat
     1501 cgatgcgtac ccagcctccc cgacacgaat cccgcgggat ccctgagctc gcaaaccccg
     1561 gcgcacgcac gaagcgcgtc gaggatctcc cgcgcatcga cccgttcgcc gccacgccgg
     1621 aacagcacca gatcgaaatc cgaagcctcg gaagcgatcc gcagcccgcg cgagccgccg
     1681 atgccaaccc cgacgacccg atcgagcccc cccagcgcgc ggatcagctc atccatgccc
     1741 gaggcctccg ccggtccctg tcatgcccgg ctcaatccag cccgctcgcc cgcaagccgc
     1801 tggtgatgcc cttcagcgag ccggccacct tcacctgctg cctgccggcg ttgccgtaga
     1861 actcgaggtt gagcacgcgg ccggccttca gtgcctcgat catgcccgcg tcgagcggca
     1921 ccgtggcgat gcagccctgc gcgacacagg tcagcggtgt catggcgaag ccgttcttgc
     1981 catcgacgtc gccccggatg ccgttgggca ggtgaatgcc gagcggcacg accacactca
     2041 tgccgacccc gccgccctgg ggcggacggc tgatgttgaa gcgcaccgag cgcttatccc
     2101 cctcgccgaa ggaatgcacc tgcaccagcg cacaggcctt gctcgctccg gaaagcgcct
     2161 ggcattcgta gacccagtcg ccgaaacgct ccccctgcgc gacctgcgcg cgcaacatgg
     2221 agggggcgag caagagcgac agggacagca gcagcggaat cgcgcgcggg gctcgggcct
     2281 tgagcattgg acagctcctc tggtcggggt gaaagcgtgt tttcattctt accgaagctc
     2341 gcggccactg tacagcacat ccggcagccc gcccgccagc gccaggccac gtgcggcgcg
     2401 cttcccggcg gcgggcagcg ggcacgtcgc cctacgcggc cctcgcgctg cgcatcgacc
     2461 ggcagacggc gacggcgagc agcacgaata ccgcgccgag cgccgcgagg gcgatcgggc
     2521 tggagacgaa gatcgcaaag tcgccctgcg aaattgccag cgcgcggcgg aagttgtcct
     2581 cgaccagcgg cccgaggacg aagccgatga gcacgggtgc cagatgcacc cccgatcgcc
     2641 tcatcccgta ggccaccagg ccgatggcga gggccgccca gacgtcgaag gcactgccgc
     2701 gcacaccgaa ggtcgccacc gcgatcagca cgaggacgag tggatagagt cgctggggcg
     2761 gcagccgcag gatgcgaacc cagaccccca ccagcggaac gttgagcacg agcagcagca
     2821 ggttgccgat cacgaagctc gccatcagcc cccagaagag ctccggattc tcgaccgcca
     2881 gcagcgggct gggcaccacc ccatgggccg acatcgcgcc gagcatgaga gccatggttg
     2941 cgctgccggg gatgcccagc gccagcgtgg gcacgaaagc cgtctgcgcc gccgcgttgt
     3001 tggccgcctc gggggccgcc agcccggcga tcgcgccgcg gccgaagcac tccgggcgag
     3061 cagaaagacg acgctcgagc gcatacgcgg cgatcgacgc cagggtgggt ccggtgcccg
     3121 gcaaggcacc aaacaatccg cccagcacgc ccccgcgcag caccggccct gcggcacggc
     3181 gccacgcctt ccagcgcggg agcgcgctgc gctcgccaat cccgccacgc accacaggca
     3241 aggcgccgaa catcgacagc atcacctccg aaacgccgaa gagccccatg gcgagcacca
     3301 ccagcggaat gccgtcgcgc agttccgtca gcccgagggt gaaacgggcc acgccggtct
     3361 gggcgtcggt accgaccatc gcgaggccga ccccggcgag catcgccacc atgcccgcgg
     3421 ccagacgccc gcggcccacg gcggccgtgc agaccagtcc gaagatcatg atcgcgaagt
     3481 attcggccgg cccgagcatg aacgcgagcc cggcgagcgg ggcggccatg gcgagcatca
     3541 tgccgatgcc cacgaccgcg ccgccgaagg acgctcccgc actcaggatc agcgcctcgc
     3601 gggcacgccc cgcgcgcgcc atcgggtgac cgtcgaggca tgaaaccgcg gaagcgggcg
     3661 ttcccggcac gttgagcagg atcgccgcga tcgccccgcc gtactcggag ccgtaataga
     3721 cccccgccaa cagcaccagc gccgcggtcg gcgacaaggc atgcgtgaat ggcaacagca
     3781 ttgcgaccgc ggccagcgag ctgaccccgg gcaacacgcc gaccagggtt ccgagcgcca
     3841 ccccgatcag gcagaaccag agattgcccg gcgacagcgc gacctcgaac cccgccatca
     3901 ggccgctcaa ggcatccacg acacccccgc aaaaggattc ctgaaactcg ccgcggggag
     3961 ctccagcccg acgccgaaca tcgctgccac caggcccgcc agcactacgg cctgcaggag
     4021 cttcccacgc cgctcccagc gcgcatcccc agcgctcgca agcagcgcag ccgcgaaggc
     4081 ggccggcaca taacccagca cgggcgcgag cgaggcgaaa cccgccagtc cggcgaccgc
     4141 cgcccacacg ccacgcagac ccgcgtcgtc cccctcgcgc ggggtggagg aatcgtccgc
     4201 cgacatggac catggccgca agcacgccag ggctgcggcg atgagcagcg ccgcccccgc
     4261 gagcgcgggg aacgccccgg gcccgaggcg tcgcaaggag cccaggccca gcgcggcgcc
     4321 cccgagcacg agccacgcac cgaggcccga gagcgcaagc agcaccagcg tccacaggcg
     4381 cgcttggcga tccggcccct gcccccgcac gcggcggtcc tgcgtcggag tcatggccgg
     4441 cacgcccccg acggtggcgt gaggggccgc atggctctag agttcgactc tgcggtcatc
     4501 cacttcctcc cgagcgtgat tcatgaaccg acgacatttc atcggcgccc tcgccggcct
     4561 cgcggcagcg cgcgcccgag ccggagaagg aaacggcgtc cggatgatac cggagcggcc
     4621 gaccctcctc gtcgcggtcg ccgccggtgg cggccccgat tcggtcgcgc gtgcgatcgc
     4681 tggccatctc gccgaccggc acggcatcca tgcacgcatc gtcaacctcc cggacgccaa
     4741 cggcgagcgg gccctggcgg attttctcac cctgccggca gacggcagca cctggctgat
     4801 cgcggccgac agcctgatcg tggtgaatcc ccggctctac ccgcgcgagt ccgccgaccc
     4861 gctgcacggt cttgcaccgg tcgcccaggt cggcagcaat ttcttctttc tcgcggtcgg
     4921 ggccgaggac ccgatccgga gcatggccga gctcgtgctc gaggcgcggg cggcggagcg
     4981 cccactgaac tacgcgagcg gcggcatcgg cagcatgcac catctcctca tggccgacct
     5041 ggcggcacgc ctcggactgc ggctgaacca cgttccctac aagggcggcg gagccgccgc
     5101 gaccggtctc gcgcgcggcg acgtgcgagt cgcgttcgcc ggcgcctccg ccctcccgct
     5161 cgtgcgggcg ggcaagctgc ggctgctcgc agtcgcggcg ccgcaacgct cgccgcgttt
     5221 tccggatctg cccagcctcg ccgaggtggc gccgggtttc gacggcacgc tctggttcgg
     5281 ctggtacggc catgcgggca cgccggcacc gatccgggac gcgatgcggg gactgctgca
     5341 ggaggcaatg gccagtccgc aaacgagcgc cgcactcgac cgccagcacg gcggcatttc
     5401 ccccagcttc acttccgggc cggccttcga cgcgctgatc gagcgcgaac gggagcgctt
     5461 cgcccggctg atttccctcc ttccggaaca gacgcgatga gccatgcaca gcccccccgc
     5521 ccgctcgcgc ccgcaggcac cggtccgctc gccgaacctg tgctcgaatc ggctcccttt
     5581 gccctcgaga tctcgcgctt cgcctgtcgc tgcggcgcgc aaggggattc ctgccagccc
     5641 taccacggct tctggcagta ccttcgcctg ctcgggctgg gaaagacgat gagcggcttc
     5701 agcgccgaat tcgtcgccgc gatccggcag gcagcccaga cccatcgcgg cccctcgttt
     5761 cgcatcctga tcagcggctg cgccgactat tccgcctacg cccatgtgct tgccggctgc
     5821 gccggcctgt cggtgcagcc gcgcgtgacc gcgctcgacc tctgcgcgac cccgctgatc
     5881 ctgagtcgct ggtatgcacg ccggcacggc ggcgacctcg ctgcggtctg ctcggacatc
     5941 ctggaccatc gccacccggg aggctacgag ctgatcctca ccagcagctt cctgggctac
     6001 tttccccccg cccggcgcgc ggagctgttc tcgcgctacg cggcgctgct cgcgccgggc
     6061 ggtcgcctga tcttcaccac ccggctgcgt cccggcgcgg aagaccagct cgcacccttc
     6121 gggccggacc agaaggaagc gctcgtgcgc accgtcgtcg actcgctgac ggagctttcc
     6181 ggcctcgggc tggaccggag ggaagccgcg cagctcgccg ccgcttacgc cgacctttgc
     6241 gcctcgtatc cggtcaatgg cgaagcgagc ctgcgccggc tcgccgaagc cgcgggcctg
     6301 ctctggctgg actgcgaccg gcggcgctcc gaggcagccc ggcccggggt gaccgggccg
     6361 acagtcggcg cagcggaata cctcttcgtt actctgggac gtcccgcctg agcacgcggg
     6421 caaggcccct caccggcgca ggtgcggctc gatcatgggc cgtatcgcag cgagcgcgcc
     6481 gcgttcggcc gcgctcatcg ccgtctcgtc ggcgacccga aggctgtcga gcacgtcgct
     6541 cgggagaccc agccgcagga tgtcgggcgc cgacatcagg cacgccgcgg agccgacatc
     6601 ggcccggcac aacagggccc gcgccgccgc cagcgcgtcc tcgaacggct tgcttacccg
     6661 agctccgcgc aggacgaggc catgacgaga cgacacgcca tcgacctcgc cgtgctgcag
     6721 caaccgcgcc gacaggcccg cgtgccagtc ctcgcggtcc ggacagacga cgaggtggac
     6781 cgggatgcgt ccgtcgcccc cgacgagctc gcgcaccacc gcatcgagca cgcccgcgtg
     6841 ggaattgccc cccatctgcg gttggccgtt gacctcgcag atcgccccgc ccgcctccag
     6901 ccaggatctg gagacatcct cgatgatcag atccacgccg gccagatcca gccgcaacgc
     6961 catcgcggcg tccaccgcga gctgcagatt cgccggatgg acgtcgccga gcgcacaggg
     7021 gcggttttcc ccaccggcat tgatgttgtc gcgccgccgc aagcgaatgt actcgccggc
     7081 tgcgggcacc gatgcgggcc ccatgcctcg gcgcgccagc agatcgagcg cctccgcgtc
     7141 caggctcagc aggggagccc ccgcgcgctg cgcgcgccgc ctgtggatct cgtcctccag
     7201 gcgccgctcc acgagagtgg ccacagcgga cacgccatcg cccaccacgc cccccgccac
     7261 cctgcgcatc acgcggacca ccctgccctg gaagacggtc aggcggtgcg tgtagccctc
     7321 gacatggcgc tcgacgagga tcgtccgcga cagggcgctg gccgcgtcat acgcgatgcg
     7381 aaccatcgca tcgtcggaca gcccggcctg cacccccgcc ccctgctcct ggtccgcagg
     7441 cttgaccact acggggtagc ccagcgcacg ggcggcctcg accgcgcctt ccggcgagcg
     7501 cacgaccacc tgctgcggca ccgggaggcc cgcggcacgg agaacttccg cggtatgcac
     7561 cttgttgcgg gcgatcccga ggccgatgcc ggacgtccgg tcggtgatcg tgctcctcat
     7621 ccagcgcgcc ttccgcccct gaccgagcac cagtacatca tgcaggaggc gccccacagg
     7681 gatcccggcg cggtaagcgg caagtgcgat gttgatcggg ttgatgccgg ggatcgcatg
     7741 cgcgccgagc atgcgatcca gctcttccag cgactcctgc gagctgcgcc cgtccttgct
     7801 cccggcctgc ccgccggtcg cgcgtcgatt cacctcggcc accacccaac gcaacgcacg
     7861 ctcggtcgcc gcaggctggt ccgacggcag cgcgaccctg aagacgcgca catccgccac
     7921 ggtccggatc agggcacgat ggcttctcca cgacaccggg attcccgctc gtcgctgcaa
     7981 cgcgccgacg cagcgaagga tgcagtcgat caggcactcg acgcccgagg cgcccgccgc
     8041 ccgctccccg cctgtcgacg cttcgtcggc gaacgccgcc agcatctcct cgacgatgcc
     8101 ggcacgcccc tcgtccgtca acacgagttc tccggcgagt gcgccctgtt gcaaaccgaa
     8161 ggcggctccg cgcagccatg tgaagcgggt gcccacccga accaattcga ccatgatctc
     8221 ccctgccgtc cgatggaatc ctcacccgcc ccggcggttc aagacgcagt cccgctggcg
     8281 tcccaggcgc ggcacagggc gatcagcccg gccacctccg catccgcggc ctgccgcgct
     8341 ggcgaaccgg ccgtccggat gtcgccgtcg atcacgacga cgcggtcgac ccgatccagc
     8401 gggatccccg tccgcagcag ctcgagcgtc tgcacgacaa ggaccagcgc gcccacctcg
     8461 gcgcgcagcg tcaaggcacg cacccttgca tgcagcccat cgaccgccag gccgaagggt
     8521 tggccgtcgc cgcccagggt acgcacgtca ctgctcagga ccgcggccat gccggcctgg
     8581 agcaaggtcc gccagcgcgc tgcagccgcc tcccaggcgg ccgccccgcc cacgaatacc
     8641 tcgacgggaa tccgcccacg cccatcgacc aggcggaaca ggtattcctc gacgtagcgc
     8701 cgcgatattt cgccaccgcc cagcagggga gcgaaattca cctcgttgag gatggcgccg
     8761 ttggcatgcc acggcgcggt gatgtcgggg ctgatcatgt ccaccccggc aacctccagg
     8821 ccgaagaggc gtgcggcggc aatcgcggca cgcacgttct cgggatgcag cgcatgggtg
     8881 acttcctcat ccacgcctcc cgaggccgtg gtctcgatac gacgcaaggc gacgaaccgg
     8941 ccggcacccg gcacgtcctc cggccccagg ccctggcgcg cgagcatctc gagcgcgctg
     9001 tcgtccagcg cgcaaagcgg cgcgcgcttc cacggtggca aacccatccg acgcgcgcac
     9061 tcggcatcga ccaggctccc gatcgaagtc ctgccattcc cgtacacgcc ggccgggagc
     9121 cgcctcacgg catacagcag acgtcccgca gcaacgaaga tccgatggca gacccccggc
     9181 gcctgtcgct ccaccagaac ccgcttgccc ggcgagcatt cacgagccgt cgtgtaagcc
     9241 cgcgccactc cctcgtcatc gcccacatcc acgctcaccc cttcgccgcg atcccgatcg
     9301 acgggcttga cgaccaccgg ataacccagg ctgcgggctg ccgcgcgcgc ttcttccgcc
     9361 gtcatgacga gccggttcac cggcgccggc agtccggccg cacgcaacag ttcggacgcc
     9421 atccatttgt cgtgtgtcca gcgcatgccg aagacggagt cgccatcggt cgtgctgcgc
     9481 tcgatacgcc tcgccctgct gccccagccg agctgataca cgccccttcc aaggggccgg
     9541 aagggaatat ccagccgata ggctgcgcgc aagacctcga gggtcgactt tcccgcgggc
     9601 atcagctttc catggggctg cagaagctcg tctccgatgg ccacgaagac acgctcgcgg
     9661 tcctgcgggc tgcccggatc cgcgccctgt gcaagctgtg cgatgcggaa tgcgatcccg
     9721 accacctcca tgcacgccgt agcggagacg aactcgggtc ccggacacag gaacgccgcc
     9781 cgccaccgcc gccgatgttc gtcgaccgga gcgcagtcga tgagctcgaa gacgtcgaac
     9841 accggcacgc ggaccaactg caggaacagc cgcgccagac gcaggacctg ctgcagccag
     9901 taccgggccg tgtctgcgtc ttcgcacccc tcggacggcc tcgtcccgag cggggcgtcc
     9961 tcgcccagca aggccatcca ggcgtccaga cggtttgtgg cgggcgcgat cggcggtgcg
    10021 aactcgatct cgaagcgggt ctgcaactga cgcggtctcg accagtggcg gcgcgcccct
    10081 tgcgcccgac cgccctcgtg tgcccaatcg tggcgctcca gaaccgcctc gaccgcttcg
    10141 cgctgcacct tgccttgttc gctccgcggg atgcggtcga cccagatcac gtcgtgcagg
    10201 gcatggcgcc ccaaggctgc gcggacatgg gcgacgagtt catgcgcgct cggatgcgtt
    10261 ccagcttcgg gaaccaccag cgcgaccggg acatcctgca gcacgcgatg ccgcaggggc
    10321 gtggccagcg catcggccac gcccggatgc cgccgcaggc actgctcgat ctcggctgga
    10381 tggagattca cgccaccgac gatcatcatg tcgtccatgc gccctctgtg caccagctgc
    10441 ccgtcggtcg tcagatggcc gatgtccccg ggatagaacc agccctccgc gaaggccttg
    10501 cggttcgcct cctcgtcacc gagataaccg tcgatgacca tcggcgaggc gatccgcacc
    10561 cgtccgtcca cccctgccgg gaccggctgc ccgcgctcat cgaccacctc gatgcggatg
    10621 ccgggaaaag gacgccccac gccgccgctc gtccggtgaa cctcgtccag tcgagtcagg
    10681 gtcgcggtgt ggctctcgtt cgtcccgtag aagacatgga tgcggggggt caagcgtcgc
    10741 cggatctcgt cgcgcagcgc catcgacacc gtcgagcccc ccaccatcag cgcctgcagc
    10801 cgggcgaacg cgccggtcgc ctccgccgga agccgacgca ggagggtctc gatgtggcag
    10861 accatggcgt ggatcaccgt cacctcgccc tcggcgactt cacggcgaag gtccacgcgg
    10921 ccgggggtgt ccaggaagat tcccgccccg atcgccatcg cctccaggca gcgctgcttc
    10981 gcggaataga aatgcatgct caccatggag cacaagacgt cctgcgcgcc atagggcagc
    11041 cagctcggcc ccagcctcga gcgcaggcat tgctgccggt gcgtgaccgg catgatcttg
    11101 ggtcgccccg tggagccgga gcccgtcaca tagatccacg gccgatccgc cacctcgcgc
    11161 gccggctcgg gcgtcatcga cggcgggctt ctctcgagca tggaccaggc gaggatcggg
    11221 agcccgccga ggtccgctcc agttcgacga ccgtcgtcgg cgagcgcccg accagcccgc
    11281 gtgagcgcca acagtcgccc acgcaacgca tcgggcaggg tgcgcgggac actcaccacc
    11341 gtcgcccccc gatgagcgac cgccaaggag gccaccagat gatcgagggg gtcgtccaga
    11401 catagcgcga gcacgtcccc tcgcgccact ccggcgttcg ccaggcccgc ggccaccgag
    11461 cacaccctcc gccccaaggc cccatagccg agcacttcat cgccatggcg cacggcgagg
    11521 gcctccgggc gactcgaagc ctgctgcaga agcagatcga caacattcat gtcctgcgcg
    11581 aatccttgag cgggaaaaca aggctgcgtg cggctcagcc cgagcgcccg atcatatgcg
    11641 cattccaccg gctggagtgc cgcgaccgcc accgcaggcg acccgtcgcg gcggagagcc
    11701 cgcagcgcca cgcatggtag actcgcgccg ccttcagaca cccgacctcg atgcagatcc
    11761 agctcctgat ccccggcctg ctatggcctg tcgcaacctt gctcgggccg gcctccggac
    11821 tcgcgctcga gggtctcgcg accctgctcg gccgcggccg ccgggagctg accgccttcg
    11881 agccttacga tcgccagctc gcacgcctgt tcggcgtgcg cggcgacacc ctgccgatgg
    11941 cgaccctgcg ccgcctgggc gaggccgatg cgccggcgcc cgagcccggc aggcactggc
    12001 tgtgcgcgga tccggtgaac ctgtcgttcg cccgcgaaca cctgctgctg caggcttttc
    12061 cggacgaaga gctggacgcg gcggagagcg ccgagctggt cgccgagctc aacgcgatct
    12121 tcgccgacct cggccgcttc gaagcctgca cgcccacgcg ctggtacctg cgcctgcacc
    12181 gcccgaccgc ggtcacgctc tacccgctcg acgacgtgac cgggcgcccg gtcaagcact
    12241 tcctgcccga aggcgaagat gcccggttgt ggcagcgcac catgaacgag gcgcagatcg
    12301 tgctgcacaa ccacgcgcgc agccgagccc gcgaggaggc cggccgccgc gcggtcaaca
    12361 gcgtctggct atggggcgcg ggcgcgctcg atgcgccgcc gcgggcgccc gcccgcctgg
    12421 tccaggcgag cgatccggtc agcatcggcc tcgcgcgcgc taccggggtg gcgttcggtg
    12481 cgccggatcc cgctgcagcg cttgcgcagg acacgctggt cgtcctcgac gagctgcgca
    12541 agcccgcaca gcagctcgac ctcgacacct ggcggcgcgg cctcgaggcg atggagcgcg
    12601 actggttcgg cccgctcgcc gaggccttcc gcgccggtcg catcgacacc ctgcgtctga
    12661 ccgcccccgg cgatcgcggc acgctgcaac tcgagctgcg cgccggcgaa cgctggaagt
    12721 tctggcgcaa gccctacgcc ttcgacgcgc tgctgaagtc catcgccccc gcgccgatgc
    12781 agatgcccga cgccccgcgc cccgcccatg gcgccctgta gtcgcagccg atgatcgcga
    12841 cttgcgtcgc tcctgcggca gcccgaatgc gcgacacgtt ctgtaggagc ggcgcaagcc
    12901 gcgattgccg cggctccgga tgcgcggcgc cgaccgcaag gccggtgatc gcgacttgcg
    12961 tcgctcctgc ggcagtccgg atgcgcggcg ctttccgtag gagcggcgcg agccgcgatt
    13021 gccgcgccgc cgacgcgccg cggcttgtgc atgaacaccg gccacctaca agctttcttc
    13081 gaccgatgac ccgaatccag
//
LOCUS       DAOQNN010000041        12875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2077_length_12875_cov_11.026365, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000041 DAOQNN010000000
VERSION     DAOQNN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12875
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2077_length_12875_cov_11.026365"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKV97_04315"
     CDS             complement(<1..334)
                     /locus_tag="PKV97_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HNB05132.1"
                     /translation="MSLPPGYRFFAPGLLCPQWSLPPGVRAVVSTREGGASLPPWASF
                     NLGSHVGDDARAVAANRARLGAAIGAEPVWLEQVHGCAVADADRAAGVPEADAAVARP
                     VEQVVAEQA"
     gene            complement(331..1314)
                     /gene="rluD"
                     /locus_tag="PKV97_04320"
     CDS             complement(331..1314)
                     /gene="rluD"
                     /locus_tag="PKV97_04320"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HNB05133.1"
                     /translation="MNEHADYIPAALPADLPADDADAWEALTIPVELGGLRLDQALAR
                     LLPQHSRSRLQGWLKEGWIRVDGASPDARRKVCGGECVEIDAPPPPEVTAALPEDIPL
                     AVVYEDEDLLVIDKPVGLVVHPGSGNWSGTLLNALLHRNPALAGVPRAGIVHRLDKDT
                     SGLMVVAKTLAAQTELVRQLQARTVGRHYRALVQGALTAGGTVDAPIGRHPTQRTRMA
                     VVGNGRPAVTHYRVTERFPRATLVECRLETGRTHQIRVHMAHVGHALVGDPLYGARRC
                     GDERLDAFPRQALHAFRLGLTHPRSGEAMRWEVPLAADFAALLDELHGAPR"
     gene            1328..2137
                     /locus_tag="PKV97_04325"
     CDS             1328..2137
                     /locus_tag="PKV97_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNB05134.1"
                     /translation="MARFTPGSLTAKAALIGALLLGGCGLLPEQVDETAGWNAQKLYS
                     EAKASMSEGGYDRAVTLFEKLEARYPYGRFAQQAQLEVAYAYYKQGEQALALAAADRF
                     IKLHPNHPNVDYAYYLKGLVNFNEDLGLLAGLSRQDLSERDPKGAREAFDSFRELVER
                     FPESRYADDSRARMQYLINSLASHEVHVSRYYYNRGAYVAAINRAQTAVNNFPQAPAI
                     EEALFLMVMSYDKLGMAQLRDDADRVMRKNFPDSVYFRGGPEDTRPWWQLW"
     gene            complement(2327..4510)
                     /locus_tag="PKV97_04330"
     CDS             complement(2327..4510)
                     /locus_tag="PKV97_04330"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HNB05135.1"
                     /translation="MTERVSVQRLQVAANLKRFIEEEALPGSGVDAAAFWSGFDALVH
                     DLAPENAALLAERDRIQAEIDAWHRAHPGPIADMAAYKAFLSSIGYLLPVPAGAKATT
                     TNVDDELAVQAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAIPQKDGAELTKGY
                     NPVRGAKVIAFGRQVLDQAAPLAGASHADAAGYAVEAGRLVVKLQSGASTGLQQAEKF
                     VGFQGEAAAPRAVLLKNNGLHIEIQIDRSGAIGKSDAAGVNDLLMEAALSTIMDCEDS
                     VAAVDADDKVVVYRNWLGLMDGTLEDTFDKGGKPMTRRLNADREYTGADGKPVKLHGR
                     ALLFVRNVGHLMTNPAILWGEGKEIPEGIMDAVVTTLIAKRDLERRGNSRKGSIYIVK
                     PKMHGPAEIGFADELFTRVEQLLGLPANTVKLGIMDEERRTSVNLRACIAAAPARVAF
                     INTGFLDRTGDEMHTSMEAGAMMRKGDIKNSKWIAAYERRNVLIGLAAGLRGRAQIGK
                     GMWAMPDLMAAMLEQKIGHPKAGANTAWVPSPTAATLHALHYHQVSVAAVQQDIEKLD
                     LDKEAEALLDDLLTIPVVAKAEWSETERQQELDNNCQGILGYVVRWVEQGVGCSKVPD
                     INDIGLMEDRATLRISSQHIANWLRHGVVPAEQVEATLERMAAVVDGQNAGDPLYRPM
                     APNFDDSVAYQAARALIFEGCAQPSGYTEPLLHRYRQQFKAKVGA"
     gene            4802..5197
                     /locus_tag="PKV97_04335"
     CDS             4802..5197
                     /locus_tag="PKV97_04335"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNB05136.1"
                     /translation="MQILQPAGWKRPKGYANGVAASGRMLFVAGQVGWDADEVFRSED
                     LVEQIRQALRNVLAILDAGGARSTDIVRMTWFLGSAAEYNARLEEIGTVYRDLIGHHY
                     PAMSAVQVAGFVEAGAKVEIEVTAVLPDD"
     gene            complement(5288..6001)
                     /locus_tag="PKV97_04340"
     CDS             complement(5288..6001)
                     /locus_tag="PKV97_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB05137.1"
                     /translation="MAEMLLEARDLHTYYGSSHILHGVSFSIRKGEALGLMGRNGMGK
                     TTLIRSMLGLVRPRHGTVRVNGDDMTRAKPHRIALAGVAYVPEGRGIFPNLSVRENLV
                     MAARAGVDGRREWTFERVMETFPRLGERIDNGGGQLSGGEQQMLTIGRALMTNPDLLI
                     LDEATEGLAPLMVKEIWKVIRTIRDTGIATVVVDKNHSAVTALTNRSVILVKGEVVFD
                     GPSEQVRQDPALIRKHLGV"
     gene            complement(5994..6749)
                     /locus_tag="PKV97_04345"
     CDS             complement(5994..6749)
                     /locus_tag="PKV97_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB05138.1"
                     /translation="MSEAMLVADRLTRHFGGLTANLEVCITLERGKLHALLGPNGAGK
                     STCINMLSGDLPPSAGRILLEGQDVTGLDSAARSHRGIGRSYQRTNIFPQFTVLENCR
                     LAAQSRRPRPWRIFTDAMKTRDTVERAQAAIVEAGLGGRELRKAGLLSHGEQRQLEIA
                     MVLATDARVLLLDEPLAGMGSEESAQMVELLRKLKQNRAILLVEHDMDAVFAVADTIT
                     VMVEGSVLESGPPEQIRNSEDVQAAYLGEDEHG"
     gene            complement(6742..7710)
                     /locus_tag="PKV97_04350"
     CDS             complement(6742..7710)
                     /locus_tag="PKV97_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB05139.1"
                     /translation="MNPRVVLSFKLAVLAGLVLFPAIGSSFYVELVAKILVMSIFAMS
                     LALLVGFTGLVSLGHAAYFGIAAYTVALIAPEYEAANLWFVLAASVLAAGAAAFFIGL
                     FVLRTKGIYFIMVTLAFAQMAYYIFHDTALGRSSDGIYLNVKPDASLFGWMPFNLDEP
                     LHLYYFILAAMVAVFVLLAFVLRSPFGRVLAGIHSNEHRMKSLGYATQRYKLAAFTLA
                     GALAGVAGFLYAVLFGFVTPDYLSWHQSGNVLLMVILGGLGSLAGAVAGAFAFIGLQE
                     MFSDWTKHWQLLMGLVIVAAVLFMPSGLAGLPRRIFRTVNWGEDDE"
     gene            complement(7715..8581)
                     /locus_tag="PKV97_04355"
     CDS             complement(7715..8581)
                     /locus_tag="PKV97_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB05140.1"
                     /translation="MDFTSFLIQILNALQYGLLLFLVASGLTLVFGIMGIINLAHGSF
                     YMIGAYLAFALTSLTGNLMLAIVLGIPLAFGFGALLEKLLFVHLYKRDHLQQVLLTYG
                     LILIFEELRSLTVGDDVHGVAIPDLFSASIALSDNLSYPVYRLVISVVCIALAVGLWW
                     LMQRTRLGMMIRAGSFNREMVQSLGVNIDLLYRTVFALGVALAALAGMLAAPISSVYP
                     GMGGNVLIISFVVVVIGGIGSVWGALVAALLVGFADTFGTILLPELAGLAVYLLMAVV
                     LLWRPEGIFRKG"
     gene            complement(8695..9882)
                     /locus_tag="PKV97_04360"
     CDS             complement(8695..9882)
                     /locus_tag="PKV97_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB05141.1"
                     /translation="MVGKDRKLRLAVSALGLGLGMLLAQGAAEAADKVKVGLMLPYTG
                     TYASLGNAITNGFKQYVAEQGGKLGGREVEYFVVDDESDPAKATENANKLVKRDNVDV
                     LVGTVHSGVALAMAKVARDSKTLMIIPNAGADELTGPLCAPNVFRTSFSAWQPANAMG
                     KVVAERGHKNVVTLAWKYSFGEQSVAGFKESFEQAGGKVVKELYLPFPNVEFQPFLTE
                     IANLRPDAVFVFFSGAGAAKFVKDYEAAGLKAGLPLYAPGFLTDGTLEAMGGAGEGVL
                     TTLHYADGLDNAREKSFRSGYVAAYKAQPDVFAVQGYDSAQLLAAGLSGAPAGAFDKE
                     AVMKAMSAATIDSPRGSFTLSKANNPVQDIYLRKVEGGQNKVIGVAAPKLADPARGCK
                     LMN"
     gene            complement(9944..11530)
                     /locus_tag="PKV97_04365"
     CDS             complement(9944..11530)
                     /locus_tag="PKV97_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HNB05142.1"
                     /translation="MHTLSTADHGCTPPRIVIPDPYNAADDLLGRNLVAGRGSKVAYI
                     DDAGSYTYDQLAARANRCGNALLGTLGLARGDRVLMCVLDCIDFPAMFLGAIKAGVVP
                     IAVNTLLTERDYEYMLSDSGARVAVVSQALLANFAALPGRVPTLERLVVAGGDDDDGL
                     EALLAAQSEQLDAAPTRATDPCFWLYSSGSTGAPKGTVHIHSNLIHTAELYGQEVLGI
                     REQDVVFSAAKLFFAYGLGNGLTFPLSVGATAILMAERPTPDAVFKRLREHQPDIFYG
                     VPTLYAGMLAHTGCPTAGEMKRLRACTSAGEALPEGVGRRWKERIGVDILDGIGSTEM
                     LHIFLSNRAGDVQYGTSGTPVPGYRVRLIDEGGDEITAAEEPGELQISGPSSAVQYWN
                     QPEKSRATFLEGWTRSGDKYYRNRDGYYVYSGRSDDMLKVSGIYVSPIEVESALIAHE
                     AVLEAAVVGIEDEERLIKPKAFVVLKPGYGPGEAMRQELQAHVKGMLAPYKYPRWIEF
                     VEDLPKTATGKIQRFKLRGI"
     gene            11905..12837
                     /locus_tag="PKV97_04370"
     CDS             11905..12837
                     /locus_tag="PKV97_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB05143.1"
                     /translation="MNDQNPTLAASDAPVPVAHPAVRPDPRSDGEFLLALGRRVRETR
                     DRRGLTRKQLARDAGVSERHLAHLEAGEGNVSIVLLRHIASALGLSLPEMLNLGAEES
                     VEQRLVRRILEQLPRHRLEDVVFRLMRDYGQEEAARRNRIALIGLRGAGKTTLGRRLA
                     AELGFRFVELDAEIERETGMPMGEIFALYGQSGYRRIEQRCLRRALDSGERTVLATGG
                     GIVSQTETYDLLLSRCLTIWLRTSPEEHMQRVSAQGDLRPMAGSAEAMEDLRRILAAR
                     EPQYLRADHVVDTSGQGEDESFRALRAAVEDRGR"
BASE COUNT         2132 a         4386 c         4335 g         2022 t
ORIGIN      
        1 gcgcctgctc ggcgacgacc tgctcgaccg gccgcgccac ggccgcatcg gcctcgggca
       61 ctccggccgc gcgatcggcg tccgccaccg cgcagccatg cacctgctcg agccacaccg
      121 gctcggcgcc gatcgccgca cccaggcgtg cgcggttggc cgccaccgcg cgcgcatcat
      181 cgccgacatg gctgccgagg ttgaagctcg cccacggcgg caagcttgcg ccgccctcgc
      241 gggtgctcac cacagcccgt accccgggcg gcaggctcca ctgcgggcac agcaggccag
      301 gggcgaaaaa gcggtagccg ggcggcaggc tcatcgcggc gctccatgca gctcgtccag
      361 cagcgcggcg aaatccgccg ccagcggcac ctcccaacgc atcgcctcgc cgctgcgcgg
      421 atgcgtgagc cccagccgga aggcatgcag ggcctggcgc gggaacgcat cgaggcgctc
      481 atcgccgcag cggcgcgccc cataaagcgg atcgccgacc agcgcatggc cgacgtgcgc
      541 catgtggacg cggatctggt gggtacgccc ggtctccaga cgacattcga ccagggtcgc
      601 acgcgggaaa cgctcggtga cgcggtaatg cgtgaccgcc ggacggccgt tgccgacgac
      661 tgccatgcgc gtgcgctggg tagggtgacg cccgatcggc gcatcgaccg tgccgcctgc
      721 cgtcagcgcg ccctgcacca gcgcgcggta gtggcgcccg accgtgcgcg cctgaagctg
      781 acgcacgagc tcggtctgcg cggccagcgt cttcgccacc accatcagcc cgctggtgtc
      841 cttgtcgagc cggtgcacga taccggcccg cggcaccccg gcgagcgcgg ggttgcggtg
      901 cagcaaggcg ttgagcaggg tcccgctcca gttgccgctg ccgggatgca cgacgaggcc
      961 gacgggcttg tcgatcacca gcaggtcctc gtcctcgtag accaccgcga gcgggatgtc
     1021 ctcgggcagc gcggcggtga cctcgggcgg cggcggcgcg tcgatctcga cgcactcccc
     1081 gccgcacacc ttgcggcgcg catcggggct cgcgccatcg acgcggatcc agccctcctt
     1141 gagccagccc tgcaggcggc tgcgcgagtg ttgggggaga agccgtgcca gggcctgatc
     1201 gaggcgcagc ccacccagtt cgacgggaat cgtcagcgcc tcccacgcat cggcgtcgtc
     1261 ggcgggaaga tcggctggga gggcggctgg gatataatcg gcgtgttcat tcacgcgagt
     1321 gtcttcgatg gccaggttca cccccggaag tttaacggca aaagccgcgc tgattggcgc
     1381 cttgctgctc ggcggctgcg gcctcctgcc cgagcaggtc gacgagaccg ccggctggaa
     1441 cgcacaaaag ctgtattccg aggccaaggc ctcgatgagc gagggcggct acgatcgcgc
     1501 ggtgacgcta ttcgagaagc tcgaggcacg ctatccctac ggccgcttcg cccagcaggc
     1561 ccagcttgag gtggcctacg cgtactacaa gcagggcgag caggcgctcg cgctcgccgc
     1621 ggccgaccgc ttcatcaagc tccatcccaa tcacccgaac gtcgactacg cctactacct
     1681 caagggcctg gtcaacttca acgaagacct cggcctgctc gccggcctgt cgcgccagga
     1741 tctctccgag cgcgacccca agggcgcacg cgaggccttc gacagtttcc gcgaactggt
     1801 cgagcgcttc cccgagagcc gctacgccga cgactcgcgg gcgcgcatgc agtacctgat
     1861 caactcgctc gcctcgcacg aggtgcatgt ctcgcgctac tactacaacc gcggcgccta
     1921 cgtcgcggcg atcaaccgcg cgcagaccgc ggtgaataac ttcccgcagg caccggcgat
     1981 cgaggaggcg ctgttcctga tggtcatgag ctacgacaag ctcggcatgg cgcagctgcg
     2041 cgacgacgcc gaccgcgtca tgcgcaagaa cttccccgac agcgtgtatt tccgcggtgg
     2101 cccggaagac acgcggccgt ggtggcagct gtggtgaggg tctgatcccc cgatcatgca
     2161 gatcgggcgg agcaagatgt aaaaaggcgc cctgcgaggc gcctttttac atttccagtg
     2221 ccgtcgccgc gatgcacgcg tgcagctctc catcgccgga tcgcgagtcg cgtcgctcct
     2281 gcaggagcac aggagcgacg cgactcgcgc cgagcgtgga cgacgctcag gcgccgacct
     2341 tggccttgaa ctgctggcga tagcggtgca gcagcggctc ggtgtagccg ctcggctggg
     2401 cgcagccctc gaagatcagc gcgcgcgcgg cctggtaggc caccgagtcg tcgaagttcg
     2461 gcgccatcgg gcggtacagc gggtcgcccg cgttctggcc atcgaccacc gcggccatgc
     2521 gctcgagcgt ggcctcgacc tgctcggcgg gcaccacgcc atggcgcagc cagttggcga
     2581 tgtgctggct ggagatgcgc agcgtggcgc ggtcttccat caggccgatg tcgttgatgt
     2641 cgggcacctt cgagcagccc acgccctgct ccacccagcg caccacgtag ccgaggatgc
     2701 cctggcagtt gttgtcgagt tcctgctggc gctcggtctc gctccactcg gccttggcca
     2761 ccaccgggat ggtgagcagg tcgtcgagca gcgcctcggc ttccttgtcg aggtcgagct
     2821 tctcgatgtc ctgctgcacc gcggccacgc tgacctggtg gtaatgcagc gcatgcaggg
     2881 tggcggcggt cggcgacggc acccaggcgg tgttggcgcc cgccttggga tggccgatct
     2941 tctgctcgag catcgcggcc atcaggtcgg gcatggccca catgcccttg ccgatctggg
     3001 cgcggccgcg caggccggcg gccagaccga tgagcacgtt gcggcgctcg taggcggcga
     3061 tccacttgct gttcttgatg tcgcccttgc gcatcatcgc gccggcctcc atcgaggtgt
     3121 gcatctcgtc gccggtgcga tcgaggaagc cggtgttgat gaaggccacg cgcgccggcg
     3181 cggcggcgat gcaggcacgc aggttgacgc tggtgcggcg ctcctcgtcc atgatgccga
     3241 gcttgacggt gttggccggc agcccgagca gctgctcgac gcgggtgaac agctcgtcgg
     3301 cgaagccgat ctccgccggg ccgtgcatct tgggcttgac gatgtagatg ctgcccttgc
     3361 gcgagttgcc acggcgctcg aggtcgcgct tggcgatcag cgtggtgacc accgcatcca
     3421 tgatgccttc ggggatttcc ttgccctcgc cccacaggat ggccgggttg gtcatcaggt
     3481 ggccgacgtt gcgcacgaag agcagcgcgc gaccgtgcag ctttaccggc ttgccgtcgg
     3541 caccggtgta ctcgcggtcg gcgttcagac gccgcgtcat cggcttgccg cccttgtcga
     3601 aggtgtcttc cagcgtaccg tccatcaagc cgagccagtt gcggtacacc acgaccttgt
     3661 cgtcggcatc caccgcggcg accgagtcct cgcagtccat gatggtcgac agcgcggctt
     3721 ccatcagcag gtcgttcacg cccgcggcat cggacttgcc gatggcgccg ctgcggtcga
     3781 tctggatctc gatgtgcaag ccgttgttct tcagcagcac ggcgcgcggc gcggcggcct
     3841 cgccctggaa gccgacgaac ttctcggcct gctgcaggcc ggtgctggca ccgctctgca
     3901 gcttcaccac caggcggccg gcctcgaccg cgtagcccgc ggcgtcggca tgcgaggcac
     3961 cggcgagcgg cgcggcctgg tcgagcacct ggcggccgaa ggcgatgacc ttcgcgccgc
     4021 gcaccgggtt gtagcccttg gtgagctcgg cgccgtcctt ctgcgggatg gcgtcggtac
     4081 cgtagagcgc gtcgtagagc gagccccagc gcgcgttggc ggcgttgagc gcgtagcgcg
     4141 cgttcatcac cggcaccacg agctgcgggc cggcctgcac ggcgagctcg tcatcgacgt
     4201 tggtggtggt ggccttggca ccggcgggca ccggcagcag gtagccgatc gaggacagga
     4261 aggccttgta cgcggccatg tcggcgatcg gccccgggtg ggcgcgatgc caggcgtcga
     4321 tctcggcctg gatgcgatcg cgttcggcga gcagagcggc gttctccggt gcgagatcgt
     4381 gtaccagcgc atcgaacccg gaccagaagg cggcagcgtc gacaccgctg cccggcaggg
     4441 cttcttcttc aatgaaacgc ttcaggttcg ctgcgacctg cagacgctgg acactcactc
     4501 gttcggtcat ctttcgcact cactggctcg gttgttgagg aacacgcccg catgaagggg
     4561 cgcataacct ttgaagaatg ctttcgatcg cggcgtgggt caagggtgcg aagcaaaagt
     4621 cctctataag acttgcgcta tggctcggca cgccgccaaa gcggacacca tcgcatcgtt
     4681 agccgcccaa gttctgcatt ttattgcaga atacgtccgc gaactgcact atttgacatg
     4741 ccgcccagtc gcgcatcaag cgcgtacgcg cagcgccaga aacgcactcg aggagacaga
     4801 catgcagatt cttcaacccg cgggctggaa acggccgaag ggctacgcca atggagtggc
     4861 cgcgagcgga cggatgctgt tcgtcgccgg ccaggtgggc tgggacgcgg acgaggtctt
     4921 ccgcagcgag gacctcgtcg agcagatccg ccaggcgctg cgcaacgtgc tcgccatcct
     4981 ggacgccggc ggcgcgcgtt cgaccgacat cgtgcgcatg acctggttcc tgggcagcgc
     5041 cgccgagtac aacgcccgcc tcgaggagat cggcacggtc tatcgcgacc tgatcggcca
     5101 ccactacccg gcgatgagcg ccgtgcaggt ggctggcttc gtcgaggccg gcgcgaaggt
     5161 ggagatcgag gtcacggcgg tgctgccgga cgattgagga ggacggaccg ggacgggcgc
     5221 agcgccgcct tgggcggcgc acaacgcgcg gacggggcct gccgggcccc gcccgcgtat
     5281 cgaagcatca caccccgagg tgcttgcgga tcaaggccgg gtcctggcgc acctgttcgc
     5341 tcggaccgtc gaacaccacc tcgcccttga ccaggatcac cgagcggttg gtcagcgcag
     5401 tcaccgccga gtggttcttg tccaccacca ccgtggcgat gccggtgtcg cggatggtgc
     5461 ggatgacctt ccagatctcc ttgaccatca gcggcgcgag gccctcggtg gcctcgtcga
     5521 ggatcagcag gtcggggttg gtcatcagcg cgcgtccgat cgtgagcatc tgctgctcgc
     5581 cgcccgagag ctggccgccg ccgttgtcga tacgctcgcc caggcgcggg aaggtctcca
     5641 tgacgcgctc gaaggtccat tcgcgccgcc cgtccacgcc cgcgcgcgcc gccatgacga
     5701 gattctcgcg cacgctcagg ttgggaaaga tgccgcgccc ctcgggtacg taggccaccc
     5761 cggcgagcgc gatgcggtgc ggtttggcgc gcgtcatgtc gtcgccgttc acgcgcaccg
     5821 tgccgtggcg cggccgcacc aggccgagca tgctgcggat cagcgtggtc ttgcccatgc
     5881 cgttgcgccc catcaggccg agcgcctcac ccttgcggat cgagaagctc accccgtgca
     5941 ggatgtggct cgagccgtag taggtatgga ggtcgcgcgc ttcgagcagc atctcagcca
     6001 tgttcatcct ccccgagata ggccgcctgg acgtcctcgc tgttacggat ctgctcgggc
     6061 ggtccggatt cgagcaccga gccctcgacc atcacggtga tcgtgtcggc caccgcgaac
     6121 accgcatcca tgtcgtgctc cacgagcagg atcgcgcggt tctgcttgag tttgcgcaac
     6181 agctcgacca tctgcgccga ctcttccgag cccatgccgg cgagcggctc gtcgagcagc
     6241 aggacacgcg cgtcggtggc cagcaccatc gcgatctcga gttggcgttg ctcgccgtgc
     6301 gagagcaggc cggccttgcg cagctcgcgc ccgcccaggc ccgcctcgac gatcgccgcc
     6361 tgcgcgcgct cgacggtgtc gcgcgtcttc atcgcgtcgg tgaagatccg ccacggccgc
     6421 ggcctgcgcg actgcgcggc gagccggcag ttctccagca cggtgaattg cggaaagatg
     6481 ttggtgcgct ggtagctgcg gccgatgccg cggtgcgagc gtgccgccga atccagcccg
     6541 gtcacatcct gcccttcgag caggatgcgc cccgcggaag gcggcaggtc gcccgagagc
     6601 atgttgatgc aggtcgactt gcccgcaccg ttggggccga gcagcgcatg cagcttgccg
     6661 cgctcgagcg tgatgcacac ctcgaggttg gcggtcagcc cgccgaaatg gcgggtcagg
     6721 cggtccgcca ccagcatcgc ctcactcatc gtcttctccc caattgaccg tgcggaagat
     6781 gcgtcgcggc agtccggcca gtccgctcgg catgaacagc accgcggcga cgatcaccag
     6841 gcccatcagc agttgccagt gcttggtcca gtccgagaac atctcctgca ggccgatgaa
     6901 ggcgaacgcg cccgcgaccg cccccgccag gctgccgagg ccgccgagga tgaccatcag
     6961 caacacgttg cccgactgat gccacgacag gtaatcgggg gtcacgaagc cgaacagcac
     7021 cgcgtacagg aagcccgcca ccccggccag cgcaccggcc agggtgaagg ccgcaagctt
     7081 gtagcgctgc gtggcgtagc cgagcgactt catgcggtgc tcgttgctgt ggatgccggc
     7141 gagcacgcga ccgaagggcg agcgcagcac gaaggccagc agcacgaaca ccgccaccat
     7201 cgcggcgagg atgaagtagt acaggtgcag cggctcgtcg aggttgaacg gcatccagcc
     7261 gaagaggctg gcatccggct tgacgttgag atagatgccg tccgagctgc gcccgagtgc
     7321 ggtgtcgtgg aagatgtagt aggccatctg cgcgaaggcc agcgtcacca tgatgaaata
     7381 gatgcccttg gtgcgcagca cgaacagccc gatgaagaac gccgcggcgc ccgccgccag
     7441 caccgaggcc gcgagcacga accacaggtt ggccgcctcg tattcgggcg cgatcagcgc
     7501 gaccgtgtag gcggcgatgc cgaaatatgc ggcatggccg aggctcacca ggccggtgaa
     7561 gccgaccagc agcgccaggc tcatcgcgaa gatcgacatc acgaggattt tcgccaccaa
     7621 ctcgacatag aagctggatc cgatcgccgg aaacagcacc agccctgcca gcacggcgag
     7681 cttgaaagac agcacgactc gcgggttcat cgcctcaccc cttcctgaag atgccttcgg
     7741 ggcgccagag cagcacgacc gccatcagca gatagaccgc cagtccggcg agctcgggga
     7801 gcaggatcgt gccgaacgtg tccgcgaacc ccaccagcag cgccgccacc agcgcgcccc
     7861 acaccgagcc gatgccgccg atcaccacga ccacgaagga gatgatcagc acattgccgc
     7921 ccatgcccgg gtacaccgag gagatcggcg ccgcgagcat gccggccagc gcggccagcg
     7981 ccacgccgag cgcgaacacg gtgcggtaaa gcaggtcgat gttcaccccg agcgactgca
     8041 ccatctcgcg gttgaacgag ccggcgcgga tcatcatccc cagtcgggta cgctgcatca
     8101 gccaccacaa gccgacagcg agcgcgatgc acaccaccga gatcaccagc cggtacaccg
     8161 gataggagag gttgtcggag agcgcgatcg aagccgagaa caggtcgggg atggccacgc
     8221 cgtggacgtc gtcgccgacc gtgaggctgc gcagctcctc gaagatcagg atgagcccgt
     8281 aggtcagcag cacctgctgc aggtggtcac gcttgtacag atgcacgaag agcagcttct
     8341 cgagcagcgc gccgaagccg aaggcgagcg ggatgccgag cacgatcgcg agcatcaggt
     8401 tgccggtcag gctggtgagc gcgaaggcca ggtaggcgcc gatcatgtag aagctgccat
     8461 gcgccaggtt gatgatgccc atgatgccga acaccagggt cagcccgctc gccaccagga
     8521 aaagcagcag gccgtactgg agcgcgttca ggatctggat caggaacgag gtgaaatcca
     8581 tgattgaacc cttccctccc agacatgcgg aacacgccgg gatgcgatgg acagacgggt
     8641 ggaagcgggc tgcgacgcgg aggcggaggg cccgccaccg cctccgcgac ggaatcagtt
     8701 catgagcttg cagccgcgcg ccgggtcggc gagcttgggt gcggcgacgc cgatcacctt
     8761 gttctgcccg ccctcgacct tgcgcaggta gatgtcctgc accgggttgt tggccttgga
     8821 cagggtgaag ctaccgcgcg gactgtcgat ggtggcggcg ctcatcgcct tcatgaccgc
     8881 ctccttgtcg aaggctccgg ccggcgcacc cgagagcccc gcggcgagca gctgcgcgct
     8941 gtcgtagccc tgcaccgcga agacgtcggg ttgcgccttg taggccgcga catagccgct
     9001 gcggaaggac ttctccctgg cgttgtcgag gccgtcggcg tagtgcagcg tggtcagcac
     9061 gccctcgccg gcgccgccca tggcctccag cgtgccgtcg gtgaggaagc ccggggcgta
     9121 gagcgggagg cccgccttca ggcctgccgc ctcgtagtcc ttgacgaact tcgccgcccc
     9181 ggcgccggag aagaatacga acaccgcatc cggcctgagg ttggcgatct cggtcaggaa
     9241 gggctggaac tccacgttcg ggaagggcag gtagagctcc ttgaccacct tgccgccggc
     9301 ctgctcgaag gactccttga agccggcgac cgactgttcg ccgaaggagt acttccaggc
     9361 cagggtgacc acgttcttgt ggccgcgttc ggcgaccacc ttgcccatcg cattcgccgg
     9421 ctgccaggcc gagaacgagg tgcggaacac attcggcgcg cacagcggac cggtaagctc
     9481 gtccgcgccc gcgttcggga tgatcatcag ggtcttggag tcacgcgcga ccttggccat
     9541 cgccagcgcc acgcccgaat gcacggtgcc gaccagcacg tcgacgttgt cgcgtttgac
     9601 cagcttgttg gcgttctcgg tcgccttggc cggatcggac tcgtcatcga cgacgaaata
     9661 ctcgacctcg cgaccgccga gcttgccgcc ctgctccgcc acgtactgct tgaagccgtt
     9721 ggtgatcgcg ttgccgagcg aggcgtaggt gcccgtgtag ggcagcatca gccccacctt
     9781 gaccttgtcc gccgcctccg ccgcgccctg cgccagcaac atgcccaggc cgagcccgag
     9841 cgcgctcact gcgaggcgca gcttcctgtc ctttccgacc atgtccgtct cctctctttt
     9901 gatggtgtga acacgggcgg cgcccccgag cgccgccaaa gcactagatg ccgcgcagct
     9961 tgaaacgctg gatcttgccg gtggcggtct tgggcagatc ctcgacgaat tcgatccagc
    10021 gcggatactt gtagggcgcg agcatgccct tgacgtgcgc ctgcagctcc tgccgcatcg
    10081 cctcgccggg tccgtagccg ggcttgagca cgacgaaagc cttgggcttg atcaggcgtt
    10141 cctcgtcctc gatgccgacc accgccgcct cgagcaccgc ctcgtgggcg atcagcgccg
    10201 actcgacctc gatcggcgac acgtagatgc cgctcacctt gagcatgtcg tcgctgcgcc
    10261 cggagtagac gtagtagccg tcgcggttgc ggtaatactt gtcgccgctg cgcgtccagc
    10321 cctcgaggaa ggtggcacgg ctcttctccg gctggttcca gtactgcacc gccgagctcg
    10381 gcccggagat ctgcagttcg cccggctcct cggccgcggt gatctcgtcg ccgccctcgt
    10441 cgatcaggcg cacgcggtag cccggcaccg gcgtgccgct ggtgccgtac tgcacgtcgc
    10501 cggcgcggtt ggacaggaag atgtgcagca tctcggtgga gccgatgccg tcgaggatgt
    10561 cgaccccgat gcgctccttc cagcgccgcc ccacgccctc gggcagcgcc tcgcccgcag
    10621 aggtgcaggc gcgcagccgc ttcatctcgc ccgcggtcgg gcagccggtg tgcgcgagca
    10681 tgccggcgta gagcgtgggc acgccgtaga agatgtcggg ctggtgctcg cgcaggcgct
    10741 tgaacaccgc atccggcgtg ggccgctcgg ccatcaggat ggcggtggcg cccaccgaaa
    10801 gcggaaaggt gaggccgttg ccgaggccgt aggcgaagaa gagcttcgcg gccgagaaca
    10861 ccacgtcctg ttcgcggatg cccaggacct cctgtccgta cagctcggcg gtatggatga
    10921 ggttggagtg aatgtgcacc gtgcccttcg gcgcaccggt ggaaccggag gagtagagcc
    10981 agaaacaggg gtcggtcgca cgcgtcggcg cggcatcgag ctgctcggac tgcgcggcga
    11041 gcagcgcctc gagaccgtcg tcgtcgtcac caccggcgac gaccaggcgc tcgagcgtcg
    11101 gcacgcgccc gggcagcgcg gcgaaattgg ccaacagcgc ctgcgatacc accgccacac
    11161 gcgcaccgct gtcggagagc atgtactcgt agtcgcgttc ggtgagcagc gtattgactg
    11221 cgatcggcac caccccggcc ttgatcgcgc cgaggaacat cgcggggaaa tcgatgcagt
    11281 cgagcacgca catcaggacg cggtcgccgc gtgccagccc gagggtgccg agcaacgcat
    11341 tgccacagcg gttggcacgc gccgcgagct ggtcgtaggt gtagctgccg gcatcgtcga
    11401 tgtaggcgac cttgctgccg cgcccggcga cgaggttgcg cccgagcagg tcgtcggcgg
    11461 cgttgtaggg atccggaatc acgatccggg gcggcgtgca gccgtgatct gcggtgctga
    11521 gggtgtgcat cgtcctgtct ccatcctgat cccggaccgt accgaccggc atgcacacgg
    11581 aacggtacga cgcgagagaa ccttggcacg atcgccgagc ggactggctg gggtaccgca
    11641 caagccatcc aaaaaagtgc actaaagtgc atctttcggc gagacatgca aaagaatacc
    11701 cccaatccgg atgctgtcaa ccctgcatcg caccaaaaac aaggaaattc aattatttag
    11761 ataaagtcta aggtgcaata tactgcactt gccgctatgc ttgaatgcac ttcccgcaca
    11821 ccggccggga gcctccgatg tcgcgccgtt gacagcgcat cgccagcggc accataaaca
    11881 cgaatctccg acctccgctc cgtaatgaac gaccagaatc cgaccctcgc agcctccgac
    11941 gcgcccgtac cggtcgcgca cccggcggtg cgacccgatc cgcgcagcga cggggagttc
    12001 ctgctcgcgc tcggccggcg cgtgcgcgag acgcgcgacc ggcgtggcct gactcgcaag
    12061 cagctggctc gcgacgcggg ggtgtcggag cggcatctcg cccatcttga ggccggcgag
    12121 ggcaacgtct cgatcgtgct gctgcgccat atcgccagcg cattggggct gtcgctgccg
    12181 gagatgttga acctgggcgc cgaggagtcg gtcgagcagc gtctggtgcg gcgcatcctc
    12241 gaacaactgc cgcgccaccg gctggaggac gtggtcttcc ggttgatgcg cgactacggc
    12301 caggaagagg ccgcgcggcg caaccggatc gccctcatcg gcctgcgcgg cgccggcaag
    12361 accacactgg gccgcaggct ggcggccgaa ctcggcttcc gtttcgtcga gctcgatgcc
    12421 gagatcgaac gcgagaccgg catgcccatg ggcgagatct tcgccctcta cggccagtcg
    12481 ggatatcgcc gcatcgaaca gcgttgcctg cggcgcgcac tcgacagcgg tgaacgtacg
    12541 gtgctcgcca cgggcggcgg aatcgtctcg cagaccgaga cctacgacct gctgctgtcg
    12601 cgctgcctga cgatctggct ccgcacctcg cccgaggagc acatgcagcg cgtcagcgcg
    12661 cagggcgacc tgcgtccgat ggccggtagc gcggaagcga tggaagacct gcgccgcatc
    12721 ctcgccgcgc gtgaaccgca gtacctgcgc gccgaccatg tggtcgatac ctcgggccag
    12781 ggcgaggacg agagcttccg cgcgctgcgc gccgccgtgg aggaccgcgg ccgctgagcg
    12841 aaggcgtcgc gcgtgatcgg cttgatcggc aagac
//
LOCUS       DAOQNN010000042        12743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2114_length_12743_cov_10.233685, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000042 DAOQNN010000000
VERSION     DAOQNN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12743
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2114_length_12743_cov_10.233685"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PKV97_04375"
     CDS             complement(<1..893)
                     /locus_tag="PKV97_04375"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNB05144.1"
                     /translation="MTLLIGVPKETFAGEKRVATVPEVVAKLIKLGFSVAVESGAGAA
                     ANFSDDDYRAAGAEVSPGSAELLGRADIVFKVRPPTLDEVGMLREGATLIGFVWPAQH
                     PELMAALQARKTTVLAIDCLPRQLSRAQKMDALSSMAGISGYRAVVEAANAFGRFFNG
                     QITAAGKIPPAKVFIAGAGVAGLAAIGTAANLGAVVRANDTRAEVADQVVSLGGEFVK
                     VDYDEEGSGGGGYAKVMSEGFQAAQRQMYAREAKDADIVITTALIPGKPAPKLITAEM
                     VQSMKPGSVIVDLAAEQGGNC"
     gene            complement(1135..1431)
                     /locus_tag="PKV97_04380"
     CDS             complement(1135..1431)
                     /locus_tag="PKV97_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05145.1"
                     /translation="MDSPCSSIELQAGRIRFFGAAKQTIKNPPSAAHDGDLICFERHA
                     VPGADARTLTPGRGLASRCIGGGPGIALAIESQVGSGILAEGTNIGNRRECDRP"
     assembly_gap    1594..1693
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1762..2337
                     /locus_tag="PKV97_04385"
     CDS             1762..2337
                     /locus_tag="PKV97_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HNB05146.1"
                     /translation="MNPKPRHIERALRERVEIVPYDPAWPGLFAEEVARLRAALPAEL
                     IGRIEHFGSTAVPGLAAKPIIDMLVEVRSMEDVAERIAPLLRALGYEFFWRAPGHGLP
                     GMEYAWFIRRDAAGRRTHHIHFVEAGSSEWGRLRFRDYLRAHSEVARAYGALKQRIAA
                     QYPDDRIAYAKAKTCFVREVMRAVRRDHIPW"
     gene            complement(2322..3959)
                     /locus_tag="PKV97_04390"
     CDS             complement(2322..3959)
                     /locus_tag="PKV97_04390"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HNB05147.1"
                     /translation="MERESMEFDVLVVGGGPAGLAAAIRLKQLAEAKGSELSVCLIEK
                     GAEIGAHILSGAVIDPRSLNELIPDWKDKGAPLTTAVTEDQVLFLSETGVRRVPPALT
                     PDCFENHGNYIVRLGNLVKWLGEQAEAAGIEVYPGFAAAEILYDESGAVKGVATGDMG
                     LNRDGTPGPHHQAGMELHAKYTLFAEGCRGHLGKQLEAKYKLREGVDPQTYGIGIKEL
                     WEVKPEQHRPGLVVHTAGWPMDNRTYGGGFIYHLEDNLVALGFVVGLNYENPHLSPFD
                     EMQRWKTHAEVRKHIEGGKRLAYGARAIATGNIQSLPRLIFPGGGLIGDDAGFLNAAR
                     IKGTHTAIKSGMLAAEAAFDALAANRQRDALTAYPAAFRDSWLYAELHKYRNFKPLMK
                     KGLWMGSFLFGMDQMLFRGKAPWTLHNSSDHDKLKPASECPKIDYPKPDGVLTFDRLS
                     SVFLSNTNHEEEQPCHLQLKDPSVAIAVNLAKYDAPEQRYCPAGVYEIVREESGPRLQ
                     INAQNCIHCKTCDIKDPTQNINWVVPQGGEGPIYQGM"
     gene            4149..4568
                     /locus_tag="PKV97_04395"
     CDS             4149..4568
                     /locus_tag="PKV97_04395"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB05148.1"
                     /translation="MSPNAKLIYTTRMPVRWGDMDAYGHVNNTVYFRYFEQTRVEWLE
                     RMGSRVSVEEPVGPVIINASCTFLAPVNYPATVVIKMYAGDPGRSSVMTWYELFVDGD
                     ERLYAEGAAKTVWMDMRTGKSAPIPDVVRALFDQPVA"
     gene            4702..6690
                     /locus_tag="PKV97_04400"
     CDS             4702..6690
                     /locus_tag="PKV97_04400"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNB05149.1"
                     /translation="MYPTEQKPLWTPSPERIAAANVTAFRLAAEQRWKVSLPDYEALH
                     AWSVEHPEPFWTSVWEGDGVGGGVIGTRGERVLVDGHKMPGARWFPDARLNFAQNLLR
                     SRDAHDAIVFWGEDRVMNRMSHGELYRAVAHFAAALKEQGVVAGDRVAAYMPNLPETV
                     VAMLAAASLGAIFTSASPDFGVQGVLDRFGQTEPKVLVACDGYYYGGKTVDVLGKLGE
                     IVAQLPSVKRVVVVPYVHREHDLSHVPHARMLADFVAPFHFVDDIEFAQLPFDHPLYI
                     MYSSGTTGVPKCIVHCAGGALLQHLKEHKLHGDVKPGDRVFYFTTCGWMMWNWLVSAL
                     AAEATLLLYDGSPFAGDNQILFDYADAEAMTHFGTSAKFLDAAAKFGLKPRETHKLDT
                     VRAMMSTGSPLVPEGFDYVYRDIKPDLQLSSISGGTDIISCFVLGSPVLPVWRGEIQC
                     RGLGMAVDVWDDDGRPVRGEKGELVCAKPFPVMPIGFWNDADGSKYHAAYFDRFPNVW
                     CHGDFCEITAHGGLVIYGRSDATLNPGGVRIGTAEIYRQVEKLHEVVESLVIGQDWPP
                     QNPNDVRVVLFVKLREGLALDDDLTRRIRQTIRDNTTPRHVPAKVLQVADIPRTKSGK
                     IVELAVRNVVHGRPVKNQEALANPEALAHFRDRAELQG"
     gene            6705..7247
                     /locus_tag="PKV97_04405"
     CDS             6705..7247
                     /locus_tag="PKV97_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNB05150.1"
                     /translation="MLMNPKKSVLLVIDVQAKLAPFIHDNAAVVEACVWLARVAERIG
                     VPVVVTEHFPEKIGGTVEAVRAATAGAHYVGKQFFSAQADGCLDQTAVAGCEQVILCG
                     TEAHVCVQQTALDLRGAGKQVFIVAEASGSRAPANRDLAFERMRGHGIEIVSREMVAF
                     EWLQRGGTELFREVNRDFIR"
     gene            7464..12293
                     /locus_tag="PKV97_04410"
     CDS             7464..12293
                     /locus_tag="PKV97_04410"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HNB05151.1"
                     /translation="METREAGRLATQVEAVIEQIRTRMPAEQAEGVCAFASRFFAQVA
                     PEDLEDLPVGDLYGAVLSQWHFVARRQGGSKVRAFNPRLDEHGWECPHTVVEIAGEDM
                     PFLVDSITMEVARQGLTLHLIIHPVMNVARDAEGGFVRVAERGEQGEGVGFESIMHLE
                     VDRRTDPGDLVALQQGIEHVLADVRAAVRDWSAMRERLAGITAGLDELPVSIDAEETQ
                     EVRAFLDWLAADNFVLLGCRDYALVETGEGNELRIVPGSGLGLLRGDGDEGQSRSFAA
                     LPPQLRAQAHLPHLLTVTKSNSRSTVHRPGYLDFVGIKTFGADGRVNGERRVIGLLAS
                     TAYSASPRQIPLLRRKVEAVFERAGLLPGGHAAKALQTLLERYPRDELFQIETDELHA
                     HAMGILRLGERLRTRLFVRVDPFERFVSCLIYVPREYYNTDQRERMQAVLIDAFKGNA
                     AEFDVQFSDSALARILITVRTPEGRIPAFDVREIEQRLIRAARRWEDELQQALVEQCG
                     EERGLALMRRYGEGFPAGYREEYSARMAVFDIEQMEALADDAALGLNLYVPLEVRAGR
                     LNLRLYHLGSPVPLSQSLPMLEKMGVKVMDERPSEIERQDGSTVWLHDFGLQFAGAEN
                     LNIHDIRPLFQEAFLAAWRGAAENDDFNRLVLLAGLSWREVAVLRAYARHMRQAAFTF
                     SLAYMEQTLAAYPQFARALLQLFRARFDPALAGDREAACATQVAAIEAALDQVANLDE
                     DRILRQFLALMQATLRTNWFQRGADGAPKPYLSFKFLPSKIPNLPQPLPMFEIFVYSP
                     RFEGVHLRGGKVARGGLRWSDRMEDFRTEILGLVKAQIVKNAVIVPVGSKGGFVVKCP
                     PAEGGREALLAEGVACYRNFLRGLLDLTDNLVQGAVVPPADVVRHDEDDPYLVVAADK
                     GTASFSDYANQVSAEYGFWLGDAFASGGSVGYDHKKMGITARGAWEAVKRHFREMGKD
                     IQQEPFTVVGIGDMSGDVFGNGMLLSKQIRLVAAFDHRHIFIDPDPDPARSWEERARM
                     FALPRSSWDDYDRALISQGGGVWPRSAKSITLSPEARAALDIQAAPLGLTPTELIRAI
                     LTAPVELVYNGGIGTYIKAASQTDAAVGDRANDAVRVNGGELRCKVFGEGGNLGATQL
                     GRIEFALAGGRINTDAIDNSGGVDCSDHEVNIKILLGGVIAEGELTLKQRNELLADMT
                     DEVGILVLRDNYAQTQVLSVTRARGVALLDEQAEFIRRLVQAGRLNRKLEYLPDDDEI
                     AERKAAGGGLTSPELAVLLAYSKIELYDEVLASDVPEDPYIRTALERYFPAPLRERFP
                     AQIRIHPLRREIISTHVVNSMINRVGPTFVSRLRAELGASAADVVRAYMATREVFGLV
                     ALWREIEALDNRIADAVQTELIQESGRLVQRGTLWFLRHRRWLADLQATSAHFSPGVA
                     ALAEGLADYVAPAYRAELDAAVARRVEQGVPEALAKRVAALEELYSALDLVEVAVEQG
                     RDEATVARVYFALGGEFDLHWLGRQISGLPADTRWQSLARGALRSDLSSLARELTSAA
                     LRNAPEGADTEGVLNAGRERAAVPLERYQQLLAEIRSAPTIDMAMLSVLLRELRGMA"
     gene            12377..>12743
                     /gene="pyrG"
                     /locus_tag="PKV97_04415"
     CDS             12377..>12743
                     /gene="pyrG"
                     /locus_tag="PKV97_04415"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305926.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HNB05152.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTSAKMSKRNNFTTGQIYESVLKKE
                     RRGEYLGKTVQVIPHITDEI"
BASE COUNT         1900 a         4361 c         4365 g         2017 t
ORIGIN      
        1 tcgcagttgc cgccctgctc ggcggcgagg tcgacgatca cgctgcccgg cttcatgctc
       61 tgcaccatct cggcggtgat cagcttgggt gcgggtttgc ccgggatcag cgcggtggtg
      121 atcacgatgt cggcgtcctt cgcctcgcgc gcgtacatct ggcgctgcgc ggcctggaag
      181 ccctcgctca tcaccttcgc gtaaccgccg ccacccgagc cctcttcgtc gtaatcgacc
      241 ttgacgaact cgccgccgag cgacaccacc tggtcggcga cctcggcgcg ggtgtcgttg
      301 gcgcgcacga ccgcgcccag gttggccgcg gtgccgatcg ccgccaggcc ggcgacgccg
      361 gcgccggcga tgaagacctt ggccggcggg atcttgcccg cggcggtgat ctggccgttg
      421 aagaagcgcc cgaaggcgtt cgccgcctcg accaccgcgc ggtagccgct gatgccggcc
      481 atggaagaca gcgcgtccat cttctgcgcg cggctcagct gccgcggcag gcagtcgatc
      541 gcgagcacgg tggtcttgcg cgcctgcaag gccgccatca gttcggggtg ctgcgccggc
      601 cagacgaagc cgatcagcgt cgcgccctcg cgcagcatgc cgacctcatc cagcgtgggc
      661 gggcgcacct tgaagacgat gtcggcgcgg cccagcagct cggccgaccc gggcgacacc
      721 tccgcgccgg cagcgcggta gtcgtcgtcg gagaagttgg ccgccgcgcc ggcgccgctc
      781 tcgaccgcga cggagaagcc cagcttgatg agtttcgcca cgacctcggg aacggtcgcg
      841 acgcgctttt caccggcgaa ggtttccttc ggaactccga tcagcaaggt catgatcccg
      901 actccgcgca ggtttgattg tggaagcggg gcgcggccga gtggatggtg cgggcatgcc
      961 cggggaagtc ttgcgctgtc gctgcgcgcg ggctccccgg ggtgggtgcg aagcggattt
     1021 cacgaagcgt ggctccgcaa gccatggtcg ggtgcatcgg gttctccctg ttcggttttc
     1081 gttggtgccg gcgtgcgcgt tcgtcacgga catgcccgcc tggcccgggc gaactcatgg
     1141 gcgatcgcat tcgcgtcgat tgccgatgtt cgttccctcg gcgagtatgc cggatccgac
     1201 ctggctttcg atagccaggg cgatgcccgg gccaccgccg atgcaccgtg aagcgaggcc
     1261 gcgtcccggc gtcagcgtgc gtgcatccgc gcccggcacc gcgtggcgct cgaagcagat
     1321 caggtcgcca tcgtgcgctg ccgagggcgg gttcttgatg gtctgcttgg ccgcaccaaa
     1381 aaaccttatc ctgcccgcct gaagttctat cgagctgcaa gggctatcca tcgcacgagg
     1441 ctgcggtggt catggacgtg gaatgtgcgg cgtcccccgc gccacgcgaa cctgccggag
     1501 cagcgcggaa acgtcggaat ccaaggagat cgtcatcatg tcgatcggcg catcattcag
     1561 cgcagtcgtg cagccgaggc gggcgcgccg gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnncggtccc gatcgcgcgg ccgtctgccc ccgttggcac gctgcgaagc
     1741 ctcgaggtca cgatttcgag catgaacccc aagccccgcc acatcgaacg cgcgctccgg
     1801 gagcgcgtcg agatcgtgcc ctacgatccg gcctggccgg ggctcttcgc cgaggaggtg
     1861 gcgcggctgc gtgcggccct gccggcggag ctgatcgggc gcatcgagca cttcggcagc
     1921 accgcggtgc ccggcctggc ggccaagccg atcatcgaca tgctggtcga ggtacgctcg
     1981 atggaggacg tagcggagcg catcgcaccg ctgctgcggg cgctgggcta cgagttcttc
     2041 tggcgcgccc ccgggcacgg gctgcccggc atggagtacg cgtggttcat ccgccgcgat
     2101 gctgcgggcc ggcgcacgca tcacatccat tttgtcgagg cgggctcgtc ggagtggggg
     2161 cgcctgcggt tccgcgacta cctgcgcgcg cattccgaag tcgcgcgagc gtatggcgca
     2221 ctcaagcagc gcattgcggc ccaatatccc gacgaccgca tcgcctacgc gaaggcgaag
     2281 acgtgcttcg tgcgcgaggt gatgcgggcg gtgcggcggg atcacatccc ttggtagatc
     2341 ggcccttcgc caccctgcgg cacgacccag ttgatgttct gcgtcgggtc cttgatgtcg
     2401 caggtcttgc agtggataca gttctgcgcg ttgatctgca ggcggggccc ggattcttcg
     2461 cggacgatct cgtacacgcc ggcggggcaa tagcgctgct cgggagcgtc gtacttggcc
     2521 aggttcaccg cgatcgccac cgacggatcc ttcagctgca ggtggcaggg ctgctcttcc
     2581 tcgtggttgg tgttcgagag gaagaccgag gacaggcggt cgaaggtgag cacgccgtcg
     2641 ggcttcggat agtcgatctt cgggcactcg gaagccggtt tgagcttgtc gtggtccgac
     2701 gagttgtgca gcgtccaggg ggccttgccg cggaacagca tctggtccat gccgaagagg
     2761 aaggagccca tccacaggcc cttcttcatc agcggcttga agttgcggta tttgtgcagt
     2821 tcggcgtaca gccaggagtc gcggaaggcg gccgggtagg cggtgagcgc gtcgcgctgg
     2881 cgattcgcgg cgagcgcgtc gaaggccgct tcggcggcga gcatgcccga cttgatcgcg
     2941 gtgtgcgtgc ccttgatgcg ggcggcgttg aggaagccgg cgtcgtcgcc gatcaggccg
     3001 ccgcccggga agatcagccg cggcaggctc tggatgttgc cggtggcgat ggcgcgcgcg
     3061 ccgtaggcca ggcgcttgcc gccttcgatg tgcttgcgta cctcggcgtg ggtcttccag
     3121 cgctgcatct cgtcgaaggg cgacaggtgc gggttctcgt agttgaggcc gaccacgaag
     3181 ccgagcgcga ccaggttgtc ctcgaggtgg tagatgaagc cgccgccata ggtgcggttg
     3241 tccatcggcc agccggcggt gtgcacgacg agacccgggc gatgctgctc gggcttcact
     3301 tcccacagct ccttgatgcc gatgccgtag gtctgcgggt cgacgccctc gcgcagcttg
     3361 tacttcgcct ccagctgctt gccgaggtgg ccgcgacagc cctcggcgaa cagcgtgtac
     3421 ttggcgtgca gctccatgcc cgcctggtgg tgcgggccgg gggtgccgtc acggttgagg
     3481 cccatgtcgc cggtggcgac gcccttcacc gcgccggact cgtcgtagag gatctcggcc
     3541 gcggcgaagc cggggtagac ctcgatgccg gcggcctcgg cctgctcgcc cagccacttg
     3601 acgagattgc ccaggcgcac gatgtagttg ccgtggttct cgaagcagtc gggggtgagt
     3661 gcgggcggaa cgcgacgcac gccggtctcg gaaaggaaca gcacctggtc ctcggtgacc
     3721 gcggtggtga gcggggcgcc cttgtccttc cagtcgggga tcagctcgtt gagcgagcgc
     3781 ggatcgatga ccgcccccga caggatgtgg gcgccgatct ccgcgccctt ctcgatcagg
     3841 cacaccgaca gctcgctgcc cttggcttcg gcgagctgct tcaggcggat cgccgcggcc
     3901 aggcccgcag ggccgccgcc gacgaccagc acgtcgaatt ccatcgattc gcgttccata
     3961 ccgttctcct cgtatcttct ttcagtgggt cgcgtagcga gacgcgccat ccgcgcagtt
     4021 taacccttcg tgcgaatccc cgccgccggt gggcgcgcgc atgcgcgcgc ccaccggcgg
     4081 cggggcgaac aaacatacgg gtgcgtatgt tacattagcc cgccccatat acaagaggag
     4141 atcgtcggat gagtccgaac gcgaagctga tctacaccac ccgcatgccg gtgcgctggg
     4201 gggacatgga cgcctacggg catgtcaaca acacggtcta tttccgctat ttcgagcaga
     4261 cccgcgtcga gtggctcgaa cggatgggtt cccgcgtcag cgtcgaggag ccggtggggc
     4321 cggtgatcat caatgccagc tgcaccttcc tcgcgccggt gaattatcct gcgaccgtcg
     4381 tcatcaagat gtatgcaggc gatccggggc ggagcagcgt gatgacctgg tacgagctct
     4441 tcgtcgacgg cgacgagcgc ctgtacgccg aaggtgcggc caagacggtg tggatggaca
     4501 tgcgcaccgg caagtcggcg ccgattccgg atgtcgtgcg cgcgctgttc gaccagcccg
     4561 tggcctgaat tggcgccggc cgccgagtgc ccggccgcgc agtgcggtca cccgttccgt
     4621 ccgcgaagcg tggccgccca gtttcgaccc ccagagtttt gacccccaat catcgacaca
     4681 agaggatgga gaggagaccc gatgtatccg actgagcaga aacccctgtg gaccccgtcc
     4741 cccgagcgca tcgccgccgc caacgtgacc gccttccggc tcgcggccga gcagcgctgg
     4801 aaggtctcgc tgcccgatta cgaggcgctg cacgcctggt cggtggagca ccccgagccg
     4861 ttctggacga gcgtttggga gggcgacggc gtcggtggcg gcgtgatcgg cacgcgcggc
     4921 gagcgcgtgc tcgtcgatgg ccacaagatg cccggcgcgc gctggttccc cgacgcccgc
     4981 ctgaacttcg cgcagaacct gctgcgttcg cgcgacgcgc atgacgcgat cgtgttctgg
     5041 ggcgaggacc gggtgatgaa ccgcatgagc cacggcgagc tttaccgcgc cgtggcccac
     5101 ttcgccgcgg cgctcaagga gcagggcgtg gtggccggcg accgcgtcgc cgcctacatg
     5161 cccaacctgc ccgagaccgt cgtcgccatg ctggccgcgg ccagcctcgg cgccatcttc
     5221 acctcggcct cgcccgactt cggcgtgcag ggcgtgctcg accgcttcgg ccagaccgaa
     5281 cccaaggtgc tggtggcctg cgacggctac tactacggcg gcaagacggt ggatgtgctc
     5341 ggcaagctcg gcgagatcgt cgcccagctg ccctcggtca agcgcgtcgt ggtcgtgccc
     5401 tacgtgcatc gcgagcacga cctctcgcac gtgccgcacg cgcgcatgct cgccgacttc
     5461 gtcgcgccct tccacttcgt cgacgacatc gagttcgcgc agctgccctt cgaccacccg
     5521 ctctacatca tgtactcctc gggcaccacc ggcgtgccca agtgcatcgt gcactgcgcc
     5581 ggcggcgcgc tgctgcagca cctgaaggaa cacaagctgc acggcgacgt gaagccgggc
     5641 gaccgcgtgt tctacttcac cacctgcggc tggatgatgt ggaactggct ggtctccgcg
     5701 ctcgccgccg aggccacgct gctgctctac gacggctcgc ccttcgccgg cgacaaccag
     5761 atcctcttcg actacgccga cgccgaagcg atgacgcact tcggcacctc ggccaagttc
     5821 ctcgacgccg ccgccaagtt cgggctcaag ccgcgggaga cccacaagct cgacacggtg
     5881 cgcgcgatga tgagcaccgg cagcccgctg gtgcccgagg gcttcgacta cgtctatcgc
     5941 gacatcaagc ccgacctgca gctctcctcc atctcgggtg gcaccgacat catctcctgc
     6001 ttcgtgctcg gctccccggt gctgccggtg tggcgcggcg agatccagtg ccgcggcctc
     6061 ggcatggcgg tggacgtgtg ggacgacgac ggccgcccgg tgcgcggcga gaagggcgag
     6121 ctggtgtgcg cgaagccctt cccggtgatg ccgatcgggt tctggaacga cgccgacggc
     6181 agcaagtacc acgccgccta cttcgatcgc tttcccaatg tctggtgcca cggcgacttc
     6241 tgcgagatca ccgcgcacgg cggcctggtc atctacggca ggtcggacgc cacgctgaac
     6301 ccgggcgggg tgcgcatcgg caccgccgag atctaccgcc aggtcgagaa gctgcacgag
     6361 gtggtcgagt cgctcgtgat cggccaggac tggccgccgc agaaccccaa cgacgtgcgc
     6421 gtggtgctgt tcgtaaagct gcgcgagggg ctcgcgctcg atgacgacct gaccaggcgc
     6481 attcgccaga ccatccgcga caacaccacg ccgcgtcacg tgccggccaa ggtgctgcag
     6541 gtggccgaca tcccgcgcac caagagcggc aagatcgtcg aactggcggt gcgcaacgtg
     6601 gtgcacgggc gcccggtgaa gaaccaggag gcgctggcca accccgaggc actggcgcac
     6661 ttccgcgacc gcgccgagct gcagggctga ggaggatcga agacatgctg atgaacccga
     6721 agaagtcggt gctgctggtg atcgacgtgc aggcgaagct cgcccccttc atccacgaca
     6781 acgccgcggt ggtcgaggcc tgcgtgtggc ttgcgcgcgt cgccgagcgc atcggcgtgc
     6841 cggtggtggt caccgagcac ttccccgaga agatcggcgg caccgtcgag gcggtgcgcg
     6901 cggcgaccgc cggtgcgcat tacgtgggca agcagttctt ctccgcccag gccgatggct
     6961 gcctcgacca gaccgcggtc gccggctgcg agcaggtgat cctgtgcggc accgaggccc
     7021 atgtctgcgt gcagcagacc gcgctcgacc tgcgcggcgc cggcaagcag gtcttcatcg
     7081 tcgccgaggc ctcgggctcg cgcgctccgg ccaaccgcga cctcgccttc gagcgcatgc
     7141 gcggccatgg catcgagatc gtgtcgcgcg agatggtcgc cttcgagtgg ctgcagcgcg
     7201 gcggcaccga gctcttccgc gaagtcaatc gcgacttcat ccgctgagga tggccggcaa
     7261 ggccggccct gcaggatcac cccgtccggc ccctggcgcg tgcgcgtccc gaactaggga
     7321 aaacgtgcgt atccatgccg gacgggttgt tctagcctga ccgcttcgtt ccacggcgat
     7381 gcgatgtgcg ctccctcgac gctgtccata ccgagctcgc agaccgtccc gccgtatcca
     7441 aaagcgcaag cgggaggttg gagatggaga cacgggaagc aggccggctg gccacgcagg
     7501 tcgaggcggt gatcgagcag attcgcaccc ggatgcccgc cgagcaggcc gaaggggtgt
     7561 gtgcgttcgc gagccgcttt ttcgcccagg tggcgcccga ggacctggaa gacctcccgg
     7621 tgggcgatct ctacggcgcc gtgctcagcc agtggcactt cgtcgcccgc cgccagggcg
     7681 gcagcaaggt gcgcgcattc aacccgcggc tggacgagca cggctgggag tgcccgcaca
     7741 ccgtggtgga gatcgccggc gaggacatgc ccttcctcgt cgactcgatc accatggagg
     7801 tcgcgcgcca gggcctcacc ctgcacctga tcatccatcc ggtgatgaac gtcgcgcgcg
     7861 acgccgaggg cggcttcgtg cgtgtggcgg agcgcggcga gcagggcgag ggtgtcggct
     7921 tcgagtcgat catgcacctg gaggtcgacc gccgcaccga cccgggcgac ctcgttgcgc
     7981 tgcagcaagg catcgagcat gtgcttgccg acgtgcgcgc cgcggtccgc gactggtcgg
     8041 cgatgcgcga gcgcctggcc ggcatcaccg cagggctcga cgagctgccg gtcagcatcg
     8101 acgccgagga gacgcaggag gtgcgcgcct tcctcgactg gctcgccgcc gacaacttcg
     8161 tgctgctcgg ctgccgcgac tacgcgctgg tcgagaccgg cgagggcaac gagctgcgca
     8221 tcgtccccgg ctccggactg ggcctgctgc gcggcgacgg tgacgagggg cagtcgcgct
     8281 ccttcgccgc gctgccgccg cagttgcgcg cgcaggccca tctgccgcac ctgctcacgg
     8341 tcaccaagtc gaactcgcgc tcgaccgtgc atcgacccgg ctacctcgac ttcgtcggca
     8401 tcaagacctt cggggccgac ggccgggtga atggcgagcg tcgcgtgatc ggcctgctcg
     8461 cctccaccgc ctacagcgcc agcccgcgcc agatcccgct gctgcggcgc aaggtggagg
     8521 ccgtgttcga gcgtgccggc ctgctgcccg gcggccacgc cgccaaggcg ctgcagaccc
     8581 tgctcgagcg ctacccgcgc gacgagctgt tccagatcga gaccgacgag ctccacgccc
     8641 acgccatggg catcctgcgc ctcggcgagc gcctgcgcac gcgcctcttc gtgcgcgtcg
     8701 atcccttcga gcgcttcgtg tcgtgcctga tctacgtgcc gcgcgagtac tacaacaccg
     8761 accagcgcga gcgcatgcag gcggtgctga tcgatgcctt caagggcaac gccgccgagt
     8821 tcgacgtgca gttttcggat tcggcgctcg cgcgcatcct catcacggtg cgcacgcccg
     8881 agggtcggat ccccgccttc gacgtgcgcg agatcgagca gcgcctgatc cgtgcggcgc
     8941 ggcgctggga ggacgagctg cagcaggcgc tcgtcgagca gtgcggcgag gagcgcggcc
     9001 tggcgctgat gcggcgctac ggcgagggct tcccggccgg ctaccgcgag gaatactccg
     9061 cgcgcatggc ggtgttcgac atcgagcaga tggaggcgct cgccgacgac gcggcgctcg
     9121 ggctcaacct ctacgtgccg ctcgaggtgc gcgcgggccg gctcaacctg cggctctacc
     9181 acctgggcag cccggtaccg ctgtcgcaga gcctgccgat gctcgagaag atgggcgtga
     9241 aggtgatgga cgagcgcccc tccgagatcg agcgccagga cggcagcacg gtgtggctgc
     9301 acgacttcgg cctgcagttt gccggtgccg agaacctcaa catccacgac atccgcccgc
     9361 tctttcagga ggccttcctc gcggcctggc gcggcgccgc cgagaacgac gatttcaacc
     9421 gcctggtgct gctcgccggg ctgtcgtggc gtgaggtggc ggtgctgcgc gcctacgccc
     9481 gccacatgcg ccaggcggcg ttcaccttca gcctcgccta catggagcag acgctggcgg
     9541 cctatccaca gttcgcgcgt gcgctgctgc aactcttccg cgcgcgcttc gacccggcgc
     9601 tcgcgggcga tcgcgaggcc gcgtgcgcga cgcaggtggc ggccatcgag gccgcgctcg
     9661 atcaggtggc caacctcgac gaggaccgca tcctgcgcca gtttctcgcc ctcatgcagg
     9721 cgaccctgcg caccaactgg ttccagcgcg gcgcggacgg cgcccccaag ccctacctct
     9781 cgttcaagtt cctgccgtcg aagatcccca atctgccgca gccgctgccg atgttcgaga
     9841 tcttcgtgta ctcgccgcgc ttcgagggcg tgcacctgcg cggcggcaag gtcgcgcgcg
     9901 gcggcctgcg ctggtcggac cgcatggagg acttccgcac cgagatcctc ggcctggtga
     9961 aggcgcagat cgtcaagaac gcggtgatcg tgccggtggg ctccaagggc ggcttcgtgg
    10021 tcaagtgccc gcctgcggag ggcgggcgcg aggccctgct cgccgagggc gtggcctgct
    10081 accgcaactt cctgcgcggc ctgctcgacc ttaccgacaa cctggtgcag ggcgcggtgg
    10141 tgccgccggc ggacgtggtg cgccacgacg aggacgaccc ctacctggtg gtggcagccg
    10201 acaagggcac ggcgagcttc tccgactacg ccaaccaggt ctcggccgag tatggcttct
    10261 ggctgggcga cgccttcgcc tcgggcggct cggtgggcta cgaccacaag aagatgggca
    10321 tcaccgcacg cggcgcctgg gaggcggtca agcgccactt ccgcgagatg ggcaaggaca
    10381 tccagcagga acccttcacc gtggtcggca tcggcgacat gtcgggcgac gtgttcggca
    10441 acggcatgct gctgtcgaaa cagatccgcc tggtggcggc cttcgaccac cgccacatct
    10501 tcatcgaccc cgatcccgac ccggcacgct cgtgggaaga gcgcgcgcgc atgttcgcgc
    10561 tgccgcgctc gagctgggac gactacgacc gcgcgctgat ctcgcagggt ggcggagtgt
    10621 ggccgcgcag cgcgaagtcc atcacgctgt cgcccgaggc gcgcgcggca ctcgacatcc
    10681 aggccgcgcc cctgggcttg accccgaccg agttgatccg cgccatcctc accgcaccgg
    10741 tcgagctggt ctacaacggc ggcatcggca cctacatcaa ggccgccagc cagaccgacg
    10801 cggcggtggg cgaccgcgcc aatgacgcgg tgcgcgtcaa tggcggcgaa ctgcgctgca
    10861 aggtcttcgg cgagggcggc aacctcggcg ccacccagct cggccgcatc gagttcgcgc
    10921 tcgcgggcgg gcgcatcaac accgacgcga tcgacaactc cggcggggtc gattgctccg
    10981 accacgaggt caacatcaag atcctgctcg gcggggtgat cgccgagggc gagctcacgc
    11041 tcaagcagcg caacgagctg ctcgccgaca tgaccgacga ggtcggcatc ctggtgctgc
    11101 gcgacaacta cgcccagacc caggtgctgt cggtcacgcg cgcgcgcggc gtggcgctgc
    11161 tcgacgagca ggcggagttc atccgccgcc tggtgcaggc cgggcggctc aaccgcaagc
    11221 tcgagtacct gcccgacgac gacgagatcg ccgagcgcaa ggcggcgggc ggcgggctca
    11281 ccagccccga gctggcggtg ctgctcgcct acagcaagat cgagctctac gacgaggtgc
    11341 tcgcctccga cgtgccggag gacccctaca tccgcactgc gctcgagcgc tacttcccgg
    11401 cgccgctgcg cgagcgcttc ccggcgcaga tccggatcca cccgctgcgc cgcgagatca
    11461 tctcgaccca cgtggtcaac agcatgatca accgcgtcgg gcccaccttc gtcagccggc
    11521 tcagggccga gctcggcgcc tcggcggcgg acgtggtgcg cgcctacatg gccacccgcg
    11581 aggtcttcgg cctggtggcg ctgtggcgcg agatcgaggc cctcgacaac cgcatcgccg
    11641 acgcggtgca gaccgagctc atccaggagt cgggccgcct ggtgcagcgc ggcacgctgt
    11701 ggttcctgcg tcaccgccgc tggctggccg acctgcaggc gacgagcgcg catttctcgc
    11761 ctggcgtggc ggcgcttgcc gaggggctgg ccgactacgt cgcccccgcc tatcgcgccg
    11821 agctcgacgc cgcggtggcg cgccgggtgg agcagggcgt gcccgaggcg ctggcgaagc
    11881 gggtggccgc gctcgaagag ctttactcgg cgctcgacct ggtcgaggtg gcggtggagc
    11941 aggggcgtga cgaggccacg gtggcgcggg tgtatttcgc cctcgggggc gaattcgacc
    12001 tgcactggct cggccgccag atctccgggc tgcccgcgga cacgcgctgg cagagcctgg
    12061 cgcgcggcgc gctgcgcagc gatctgtcct cgctcgcccg cgagctcacc agcgcggcgc
    12121 tgcgcaacgc gcctgagggc gcggacaccg agggcgtgct gaatgccggg cgcgagcgtg
    12181 ccgcggtgcc gctcgagcgc taccagcagc tcctcgccga aatccgcagc gcgccgacga
    12241 tcgacatggc gatgctgtcg gtgctgctgc gcgagctgcg cggcatggcc tgagcgatcg
    12301 cgcgggaggg cacccggact gctgacgcgg ttcgggtgca tcggttatca tcgcgctttc
    12361 ccgcagtaca gctcccatga ccaaatacgt cttcgtcacc ggtggtgtcg tgtcctccct
    12421 gggcaagggc atcgccgcag cgtccctcgg ggcgatcctc gagtcccgcg gcatcaaggt
    12481 cacgcacctc aagctggacc cctacatcaa cgtcgatccg ggcaccatga gcccgttcca
    12541 gcacggcgag gtcttcgtca ccgaagacgg cgccgagacc gacctcgacc tcggccatta
    12601 cgagcgcttc accagcgcca agatgagcaa gcgcaacaac ttcaccaccg gccagatcta
    12661 cgagtcggtg ctgaagaagg agcgccgcgg cgagtacctg ggcaagaccg tgcaggtcat
    12721 cccgcacatc accgacgaga tca
//
LOCUS       DAOQNN010000043        12570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2171_length_12570_cov_12.806312, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000043 DAOQNN010000000
VERSION     DAOQNN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12570
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2171_length_12570_cov_12.806312"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /gene="uvrA"
                     /locus_tag="PKV97_04420"
     CDS             <1..1305
                     /gene="uvrA"
                     /locus_tag="PKV97_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNB05153.1"
                     /translation="VMYVLDEPSIGLHQRDNDRLLATLARLRDLGNTVIVVEHDEDAM
                     RSADYLVDMGPGAGEHGGEVVAHGTPDEVIASPRSVTGDYLSGRRSIAIPAQRTRPHP
                     ERQLRIVGARGNNLKTVEVSLPVGLLTCITGVSGSGKSTLINDTLARVAAHRLNGAAT
                     EPAPHDAIEGLQHFDKLIDVDQSPIGRTPRSNPATYTGLLTPIRELFAGVPEARARGY
                     SPGRFSFNVRGGRCEACQGDGLVRVEMHFLPDMYVPCDVCHGRRYNRETLEIRYKGRT
                     IDEVLGMTVEQALGFFSAVPNVARKLETLMDVGLGYIRLGQSATTLSGGEAQRVKLAL
                     ELSRRDTGRTLYILDEPTTGLHFQDIEMLIAVLQRLRAHGNTIIVIEHNLDVIKTADW
                     LIDMGPEGGGGGGMVVCCGTPEEVAAHPASHTGLHLRRVLGG"
     gene            complement(1376..2398)
                     /gene="galE"
                     /locus_tag="PKV97_04425"
     CDS             complement(1376..2398)
                     /gene="galE"
                     /locus_tag="PKV97_04425"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNB05154.1"
                     /translation="MAVTLVTGGAGYIGSHTCVELLGAGQDVVVVDDLSNASFEAVRR
                     IEELGGKPLTGFVEGDVRNHGVLARVFGEHEVAAVIHFAAKKAVGESVSQPLAYYDNN
                     LHGLLSVLQAMDAAGCRRMVFSSSATVYGDPASVPIREDFPTTATNPYGWTKLMGEQI
                     LRDLAASDPRWNVVLLRYFNPVGAHASGRIGEDPDGLPNNLMPFVSQVAVGRLPRLRV
                     FGDDYATPDGTGVRDYIHVVDLALGHLRALERIDELPGVTTLNLGTGRGYSVLEMVRA
                     FEAASGRPVPYDIVARRPGDIAACWADPARAEQVLGWKAQRDLQAMCVDAWRWQSGNP
                     QGYEAG"
     gene            complement(2509..2907)
                     /gene="rplQ"
                     /locus_tag="PKV97_04430"
     CDS             complement(2509..2907)
                     /gene="rplQ"
                     /locus_tag="PKV97_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNB05155.1"
                     /translation="MRHRNGLRKLNRTSSHRQAMFRNMANSLLRHELIKTTLPKAKEL
                     RRVVEPMITLGKKPSLSNRRLAFNRLRDRDMVVKLFDELGPRYATRNGGYLRILKCGF
                     RDGDNAPMAYVELLDRPDVEAVEVEEAEAA"
     gene            complement(2933..3919)
                     /gene="rpoA"
                     /locus_tag="PKV97_04435"
     CDS             complement(2933..3919)
                     /gene="rpoA"
                     /locus_tag="PKV97_04435"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNB05156.1"
                     /translation="MLMQSNALLKPRIIDVQSTSPVQARVTMEPFERGFGHTLGNALR
                     RILLSSLPGHAPTEVTIEGVLHEYSTLDGVREDIVDLLLNLKGVVFKLNGRSEVTVRL
                     AKAGEGVVTAADIEVGHDVEIINPDHVIAHLAPGGKLDMQIKVEEGRGYVPGNSRPEN
                     AESKSIGRVVLDASFGPVRRVSYLVESARVEQRTDLDRLIIDIETNGAVDPEEAIRYA
                     ARVLMDQLSVFADLEGTAPERVEAASPTIDPVLLRPVDDLELTVRSANCLKAENIYYI
                     GDLIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPAGLEKLG"
     gene            complement(3953..4582)
                     /gene="rpsD"
                     /locus_tag="PKV97_04440"
     CDS             complement(3953..4582)
                     /gene="rpsD"
                     /locus_tag="PKV97_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNB05157.1"
                     /translation="MARNLDPKCRQCRREGEKLFLKGEKCFTDKCSIERRAYAPGQHG
                     QRSGQRLSGYGVQLREKQKIRRIYGVLERQFRKVYAEADRRRGQTGENLLQLLEGRLD
                     AVAYRMGFGASRAEARQVVRHNGVLVNGKRVNIPSYVVRPGDVVELTERARGHLRVKA
                     ALEAAESRGFPEWLEVDAKAGKGTFKAYPVRSELPPTINEGLVVELYSR"
     gene            complement(4597..4986)
                     /gene="rpsK"
                     /locus_tag="PKV97_04445"
     CDS             complement(4597..4986)
                     /gene="rpsK"
                     /locus_tag="PKV97_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNB05158.1"
                     /translation="MAKTGTKVRKKVKKNVAEGIAHVHASFNNTIITITDRQGNALSW
                     ATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQECGIKNLEVRIKGPGPGRESSVRALNA
                     LGIKISSITDITPIPHNGCRPPKKRRI"
     gene            complement(4999..5361)
                     /gene="rpsM"
                     /locus_tag="PKV97_04450"
     CDS             complement(4999..5361)
                     /gene="rpsM"
                     /locus_tag="PKV97_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNB05159.1"
                     /translation="MARIAGVNIPNHKHAEIALTAIYGIGRSRAQKICDAAGIVRSVK
                     IKDLTESDMEKLRDEVARFVVEGDLRREVTMNIKRLMDLGCYRGVRHRRGLPLRGQRT
                     RTNARTRKGPRKPIAGKK"
     gene            complement(5415..5528)
                     /gene="rpmJ"
                     /locus_tag="PKV97_04455"
     CDS             complement(5415..5528)
                     /gene="rpmJ"
                     /locus_tag="PKV97_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNB05160.1"
                     /translation="MRVQASVKRLCRNCKIIRRKGVVRVICSDPRHKQRQG"
     gene            complement(5554..5772)
                     /gene="infA"
                     /locus_tag="PKV97_04460"
     CDS             complement(5554..5772)
                     /gene="infA"
                     /locus_tag="PKV97_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNB05161.1"
                     /translation="MTKEDVIEMQGEVTENLPNATFRVRLENGHMVLGHISGKMRMHY
                     IRILPGDKVTVQLTPYDLTKGRIVFRTK"
     gene            complement(5777..7102)
                     /gene="secY"
                     /locus_tag="PKV97_04465"
     CDS             complement(5777..7102)
                     /gene="secY"
                     /locus_tag="PKV97_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNB05162.1"
                     /translation="MANPAATLGSPGRFGDLKRRLLFLVGALIVYRIGAHIPVPGIDP
                     QGLAELFQSQQGGILGVFNLFSGGALSRFTIFALGIMPYISASIIMQLMTVAVPSLEA
                     LKKEGEAGRRKITQYTRYGTLVLALAQALGIAIALEGQAGLVLEPGLMFRFVTVATLV
                     TGTMFLMWLGEQITERGIGNGISMIIFAGIIAGLPAAIGGLFELVRTGAMHPFTALFI
                     CILVVLVTAFVVFVERGQRKILVNYAKRQVGNKVYGGQSSHLPLKLNMSGVIPPIFAS
                     SIILFPATLGQWFGSSEGMYWLRDIASTLSPGQPIYVMLYAVAIVFFCFFYTALVFNA
                     RETADNLKKSGAFVPGIRPGDQTARYIDKILMRLTLAGAIYITLVCLLPEFLILKWNV
                     PFYFGGTSLLILVVVTMDFMAQVQAFVMSHQYESLLKKANFKGAGLPGR"
     gene            complement(7118..7555)
                     /gene="rplO"
                     /locus_tag="PKV97_04470"
     CDS             complement(7118..7555)
                     /gene="rplO"
                     /locus_tag="PKV97_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNB05163.1"
                     /translation="MRLNSIKPGEGSKKEARRVGRGIGSGLGKTCGRGHKGQKSRAGG
                     FHKVGFEGGQMPLQRRLPKRGFVSLTRARNVEVRLSELDKLPVDEVDLLTLKQAGVIA
                     ADALSAKVVLSGSISRKVVLRGVGATQGAKAAIEAAGGSVAAE"
     gene            complement(7557..7739)
                     /gene="rpmD"
                     /locus_tag="PKV97_04475"
     CDS             complement(7557..7739)
                     /gene="rpmD"
                     /locus_tag="PKV97_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNB05164.1"
                     /translation="MSDKKIKVTLVKSVIGTKQSHRATVRGLGLRRLNHSVELADTPE
                     VRGMINKVSYLVKCEG"
     gene            complement(7743..8267)
                     /gene="rpsE"
                     /locus_tag="PKV97_04480"
     CDS             complement(7743..8267)
                     /gene="rpsE"
                     /locus_tag="PKV97_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNB05165.1"
                     /translation="MAKQQTKRAQASDERDDGLKEKMVAINRVTKVVKGGRILGFAAL
                     TVVGDGDGAIGMGKGKSREVPVAVQKAMDEARRKMRKVSLKNGTLQHTVTGKHGAASV
                     LMQPAPAGTGIIAGGPMRAVFEVMGVTDVICKCIGSANPYNVVRATINGLMAINTPAE
                     IAAKRGKSVEEILG"
     gene            complement(8280..8633)
                     /gene="rplR"
                     /locus_tag="PKV97_04485"
     CDS             complement(8280..8633)
                     /gene="rplR"
                     /locus_tag="PKV97_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNB05166.1"
                     /translation="MNKKETRLRRARKTRAKIAELKAVRLTVFRSNCHIYAQIISGCG
                     SRVLAAASTVEPDVRAQVANGGNKAAAEAVGKLIAERAKAAGVEQVSFDRSGFLYHGR
                     VKALAEAAREGGLKF"
     gene            complement(8645..9178)
                     /gene="rplF"
                     /locus_tag="PKV97_04490"
     CDS             complement(8645..9178)
                     /gene="rplF"
                     /locus_tag="PKV97_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004738786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNB05167.1"
                     /translation="MSRVAKNPVAIPQGVEVMISDAEIAVKGPLGTLRQPLSGEVSVV
                     REGDAVVCKAREGAENARAMSGTVRALVNNMVTGVTKGFERKLTLVGVGYRAQAQGDK
                     LNLSLGFSHPVVHQMPEGVKVETPTQTEIVIRGSDKQKVGQVAAEVRAYRAPEPYKGK
                     GVRYSDEVVVLKETKKK"
     gene            complement(9189..9584)
                     /gene="rpsH"
                     /locus_tag="PKV97_04495"
     CDS             complement(9189..9584)
                     /gene="rpsH"
                     /locus_tag="PKV97_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNB05168.1"
                     /translation="MSMSDPIADMLTRIRNGQQAQKASVSMPSSKLKVAIAKVLQDEG
                     YIDGFAVRDEAGKASLDVTLKYYAGRPVIERLERVSRPGLRIYKGSDDLPRVMNGLGV
                     AIVSTPRGVMTDRSARANRVGGEVICLVA"
     gene            complement(9598..9903)
                     /gene="rpsN"
                     /locus_tag="PKV97_04500"
     CDS             complement(9598..9903)
                     /gene="rpsN"
                     /locus_tag="PKV97_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNB05169.1"
                     /translation="MAKLALINREAKRAMLVEKYASKRAALVAKVKDTSLSEEERMAA
                     RLALQQLPRNANPTRQRNRCALTGRPRGVFRKFGLCRNKLREIAFRGEVPGMTKASW"
     gene            complement(9911..10450)
                     /gene="rplE"
                     /locus_tag="PKV97_04505"
     CDS             complement(9911..10450)
                     /gene="rplE"
                     /locus_tag="PKV97_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNB05170.1"
                     /translation="MARLQQVYKDEVAPALQQQFGYKSVMEVPRITKITLNMGVGEAV
                     GDKKILEHAIGDMTKIAGQKPVSTKARKSIAGFKIRDGYPIGCMVTLRGPRMFEFLDR
                     LITIALPRVRDFRGIAAKGFDGRGNYNLGVKEQIIFPEIEYDKIDALRGMNISITTTA
                     KTDEEARALLGAFKFPFKN"
     gene            complement(10460..10777)
                     /gene="rplX"
                     /locus_tag="PKV97_04510"
     CDS             complement(10460..10777)
                     /gene="rplX"
                     /locus_tag="PKV97_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNB05171.1"
                     /translation="MNKIRKGDEVLVLTGKDRGRRGTVLRRVDDERVVVEGVNRVKKH
                     VRPNPLKGEVGGIVEKEMPIHVSNVALFNPATQKGDRVGIKTLEDGRKVRFFKSNGEL
                     VNA"
     gene            complement(10789..11157)
                     /gene="rplN"
                     /locus_tag="PKV97_04515"
     CDS             complement(10789..11157)
                     /gene="rplN"
                     /locus_tag="PKV97_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNB05172.1"
                     /translation="MIQMQSKLDVADNSGARSVMCIKVLGGSKRRYAGVGDIIKVTVK
                     DAAPRGRAKKGDVYNAVVVRTAKGVRRPDGSLIKFDSNAAVLLNNKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            complement(11314..11583)
                     /gene="rpsQ"
                     /locus_tag="PKV97_04520"
     CDS             complement(11314..11583)
                     /gene="rpsQ"
                     /locus_tag="PKV97_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNB05173.1"
                     /translation="MSENVEKVRRALVGRVVSDKMQKTVTVLVERRVKHPLYGKIITR
                     SAKYHAHVEDGVAAQGDLVEIEECRPISRTKTWRVAKVLEKATII"
     gene            complement(11580..11774)
                     /gene="rpmC"
                     /locus_tag="PKV97_04525"
     CDS             complement(11580..11774)
                     /gene="rpmC"
                     /locus_tag="PKV97_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNB05174.1"
                     /translation="MKATELRAKSADELNKELIELLKAQFSLRMQHATQQLGNTSQIG
                     KVRRDIARVRTILREKAGQQ"
     gene            complement(11777..12193)
                     /gene="rplP"
                     /locus_tag="PKV97_04530"
     CDS             complement(11777..12193)
                     /gene="rplP"
                     /locus_tag="PKV97_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNB05175.1"
                     /translation="MLQPTRRKYRKEQKGRNTGVATRGAKVSFGEYGLKAIGRGRLTA
                     RQIESARRAMTRHIKRGGRIWIRIFPDKPISKKPAEVRMGNGKGNPEYWVAEIQPGKV
                     LYEMDGVDEALAREAFRLAAAKLPIETVFVTRLIGG"
     gene            complement(12193..>12570)
                     /gene="rpsC"
                     /locus_tag="PKV97_04535"
     CDS             complement(12193..>12570)
                     /gene="rpsC"
                     /locus_tag="PKV97_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNB05176.1"
                     /translation="RAMKRAMQNAMRLGAQGIKIMSSGRLNGAEIARTEWYREGRVPL
                     HTLRANIDYGVSEAKTTYGVIGIKVWVFKGEMLGRNEQPVAAEPEADNRRGRRGAPRD
                     GEGRPGRRPARRGEGRQEESRSE"
BASE COUNT         2185 a         4228 c         3837 g         2320 t
ORIGIN      
        1 gtgatgtacg tgctcgacga gccctcgatc ggcctgcacc agcgcgacaa cgaccgcctg
       61 ctggccacgc tcgcgcgcct gcgcgacctc ggcaacacgg tgatcgtggt cgaacacgac
      121 gaggacgcca tgcgcagcgc cgactatctg gtggacatgg gcccgggcgc gggcgagcac
      181 ggtggcgagg tggtggcgca cggcacgccg gacgaggtca tcgcctcgcc gcgttcggtc
      241 acgggcgact acctctccgg ccggcgcagc attgcaatcc cggcgcagcg cacgcgcccc
      301 cacccggagc gccagctgcg catcgtcggc gcgcgcggca acaacctgaa gacggtggag
      361 gtgtcgctgc cggtggggct cctcacctgc atcaccgggg tgtcgggctc gggaaagtcg
      421 acgctgatca acgacaccct cgcacgcgtc gcggcccacc ggctcaacgg tgccgccacc
      481 gagcccgcgc cacacgatgc gatcgagggc ctgcagcact tcgacaagct gatcgacgtc
      541 gatcagtcgc cgatcgggcg caccccacgc tccaacccgg cgacctacac cggcctgctg
      601 accccgatcc gcgagctctt cgccggcgtg cccgaggccc gcgcgcgcgg ctacagtccg
      661 gggcgcttct cgttcaacgt gcgcggcggg cgttgcgagg cctgccaggg cgacggcctg
      721 gtcagggtgg agatgcactt cctgcccgac atgtacgtgc cctgcgacgt ctgccacggt
      781 cgccgctaca accgcgagac cctcgagatc cgctacaagg ggcggacgat cgacgaggtg
      841 ctggggatga ccgtcgagca ggccctgggt ttcttctccg cggtgccgaa cgtggcgcgc
      901 aaactggaga cgctgatgga cgtcggcctg ggctacatcc gcctcggcca gagcgccacc
      961 accctgtcgg gcggcgaggc gcagcgcgtc aagctcgcgc tcgagctgtc gcgccgcgat
     1021 accgggcgca ccctgtacat cctcgacgag ccgaccaccg gcctgcattt ccaggatatc
     1081 gagatgctga tcgcagtgct gcagcgcttg cgcgcgcacg gcaacacgat catcgtgatc
     1141 gagcacaatc tcgacgtgat caagaccgcc gactggctga tcgacatggg cccggagggc
     1201 ggcggcggtg gcggcatggt ggtgtgctgc ggcacgcccg aagaggtcgc cgcgcatccg
     1261 gcgagtcata ccggcctgca cctgcgcagg gtgctcgggg gctgagcccc ggccgatcca
     1321 ccccaccggg cttccgcgcc cctttgcggc gcccgagcgg tgcgccgcga gttactcaac
     1381 cggcttcgta gccctgcggg ttgccgctct gccagcgcca cgcatccacg cacatcgcct
     1441 gcaggtcgcg ttgcgccttc cagccgagca cctgctcggc gcgcgcaggg tccgcccagc
     1501 acgcggcgat atcgcccgga cggcgcgcga cgatgtcgta ggggaccggt cgcccgctcg
     1561 ccgcctcgaa ggcgcgcacc atctccagca cgctgtagcc gcgcccggtg ccgaggttga
     1621 gcgtcgtcac gccggggagt tcgtcgatgc gctcgagcgc gcgcaaatgg ccgagcgcca
     1681 ggtcgaccac gtggatgtag tcgcgcaccc cggttccgtc cggcgtcgcg tagtcgtcgc
     1741 cgaaaacgcg caagcgcggc aggcggccga ccgcgacctg actgacgaaa ggcatcaggt
     1801 tgttgggcag gccgtcggga tcctcgccga tccgaccgct cgcgtgtgcc ccgacagggt
     1861 tgaagtaacg cagcagcacc acgttccagc gtggatccga ggcggcgaga tcacgcagga
     1921 tctgctcgcc catgagcttg gtccagccgt aaggattggt cgccgtagtc gggaaatcct
     1981 cgcggatcgg caccgaggcc gggtcgccat acaccgtcgc cgacgaactg aacaccatgc
     2041 gccggcatcc cgccgcgtcc atggcctgca gcaccgacag cagtccatgc aggttgttgt
     2101 cgtaataggc cagcggttgg gacaccgact cgcccacggc cttcttggcg gcaaagtgga
     2161 tcaccgccgc cacctcgtgc tcgccaaaga cccgcgcgag cacgccatgg ttccgcacgt
     2221 cgccctccac gaagccggtc agcggcttgc ctccgagctc ttcgatccgc cgcaccgcct
     2281 cgaaggacgc gttggagagg tcgtccacca ccaccacgtc ctgcccggcg ccgagcaact
     2341 cgacgcaggt atgcgagccg atgtagcccg caccgccggt caccaaggtc actgccatcg
     2401 ttccatcctc ctgtcctgtc cgcccgcggg cgcgctgcgc tcgaccctag cacgcacagg
     2461 cgaaaaaaaa ccgggcctga gcccggttct tcgtacgagt cgcgcgtttc acgcggcctc
     2521 ggcttcctcg acttccaccg cttccacgtc cggacggtcg agcaactcga cgtaggccat
     2581 cggggcgttg tcgccatcgc ggaaaccaca cttcaggatg cgcaggtagc cgccgttgcg
     2641 ggtggcgtag cgcggcccca gctcgtcgaa gagcttgacg accatgtcgc gatcgcgcag
     2701 gcggttgaag gccaggcggc ggttggagag gctgggcttc ttgcccaggg tgatcatcgg
     2761 ctcgaccacg cggcgaagtt ccttcgcctt cggaagggtc gtcttgatca gctcatgacg
     2821 cagcagcgag ttggccatgt tgcggaacat cgcctggcgg tggctgctgg tacggttcag
     2881 cttgcgaaga ccattacggt gacgcatttc aattcctcac ttgcccggcg actcaaccga
     2941 gcttttcaag cccggccggc ggccagtttt ccagtttcat gccaagcgtc agcccgcggg
     3001 aggccaacac ttccttgatc tcgttgagcg acttgcggcc caggttcggg gtcttcagca
     3061 gctcggtctc ggtacgctgg atcaggtcgc cgatgtagta gatgttctcg gccttcaggc
     3121 agttggccga gcgcaccgtg agctccagat cgtcgaccgg gcgcagcagc accgggtcga
     3181 tggtcggcga agccgcctcg acgcgctccg gtgccgtccc ctcgaggtcg gcgaacacgg
     3241 aaagctggtc catgagaacc cgggcggcgt agcgaatcgc ctcttccggg tcgaccgcac
     3301 cgttggtctc gatgtcgatg atcaggcgat ccaggtcggt acgctgctcg acgcgagcgc
     3361 tctcgacaag gtagctgacg cgacgcacgg gaccgaagga ggcatcgagc acgacgcgac
     3421 cgatggactt gctctccgcg ttctcgggac gcgaattgcc cggcacgtag ccgcggcctt
     3481 cctcgacctt gatctgcatg tcgagcttgc cgcccggcgc gaggtgggcg atcacgtggt
     3541 cgggattgat gatctcgacg tcgtgaccga cctcgatgtc ggccgcggtc accacaccct
     3601 cgcctgcctt ggcgagacgg acggtgacct cgctgcgccc gttgagcttg aacaccacgc
     3661 ccttcaggtt gagcagcaga tcgacgatgt cctcgcggac gccgtcgagc gtggagtact
     3721 cgtgaagcac cccttcgatc gtcacttcgg tcggcgcatg gccgggaagc gacgacagga
     3781 ggatgcgccg aagcgcattc cccagcgtgt gaccgaaacc gcgctcgaac ggctccatcg
     3841 tcacccgcgc ctgaacgggc gaggtgctct ggacgtcgat gatgcgcggt ttcagcagtg
     3901 cattgctttg catcagcatt tcctcggaac tagaagagga tcagcccccc gctcagcggg
     3961 agtacagttc cacgaccagg ccttcgttga tcgtcggggg cagttcgctg cgcaccggat
     4021 aggccttgaa cgtgcccttg ccggccttcg cgtccacctc gagccactcg gggaagccac
     4081 gcgactcggc ggcctcgagg gcggccttga cgcgcagatg accacgcgca cgctcggtca
     4141 gctccaccac gtcgcccgga cgaaccacat acgacggaat gttgacgcgc ttgccattga
     4201 ccagcacgcc gttgtgacgc accacctggc gcgcctctgc acgcgaggca ccgaagccca
     4261 tgcgatacgc gaccgcatcc aggcgacctt cgagcagctg cagaagattc tcgccggtct
     4321 ggccgcggcg gcggtcggct tcggcataca ccttgcggaa ctggcgctcg agcacgccgt
     4381 agatgcgccg gatcttctgc ttctcacgca gctggacgcc gtagcccgac aggcgctggc
     4441 cggagcgctg gccatgctgg ccgggcgcgt aggcgcgacg ctcgatggag cacttgtcgg
     4501 taaagcactt ttcgcccttc agaaacagct tttcgccttc gcgacggcac tgacggcact
     4561 tgggatcgag attacgagcc acttatcaac tccttgtcag atacggcgct ttttcggcgg
     4621 acggcagccg ttgtgcggaa tgggggtgat gtccgtgatg ctggaaatct tgatccccag
     4681 cgcattgagc gcacgaaccg aggattcgcg gcccgggccg gggcccttga tgcgtacctc
     4741 gaggttcttg atgccgcatt cctgcgccac cttgccggcc gcttcggccg caacctgcgc
     4801 ggcgaacggg gtgctcttgc gcgaaccctt gaagccggcg ccgcccgaag tcgcccacga
     4861 cagggcgttg ccctggcggt cggtgatggt gatgatggtg ttgttgaagc tcgcgtgaac
     4921 gtgcgcgata ccttcggcga cgttcttctt gaccttcttg cgaaccttgg ttccagtctt
     4981 agccattatc ggatcctatt acttcttgcc ggcgatcggc ttgcgcggac ccttgcgggt
     5041 acgggcattg gtgcgggtgc gctgaccgcg aagcggcagg ccacgacggt gacggacgcc
     5101 gcggtagcag cccaggtcca tcagtcgctt gatgttcatc gtgacttcac gacgcaggtc
     5161 gccttcgacc acgaagcgcg cgacttcatc acgcagcttc tccatgtcgg actcggtgag
     5221 atccttgatc ttcaccgagc gaacgatgcc ggcagcgtcg caaatcttct gagcacgcga
     5281 acggccgatc ccatagatgg cggtcagcgc gatctcggca tgcttgtgat tgggaatgtt
     5341 taccccagca atacgggcca tccgtatacc ccgaaacgaa aaacgttaaa ttctaatgcc
     5401 tgcggtcgag tagatcaacc ctggcgttgc ttgtgccgcg gatcgctgca aatgacgcga
     5461 accacaccct tgcggcggat gatcttgcag ttgcggcaga gccgcttgac tgaggcctgg
     5521 actctcatgg tcttgctcct gtttctttaa gcttcacttg gtccggaaaa cgatccggcc
     5581 cttggtcagg tcataggggg tgagctgcac ggtcaccttg tccccgggga ggatccggat
     5641 gtagtgcatc cgcatctttc cggagatgtg tccaaggacc atgtgaccgt tttcgagcct
     5701 gacgcggaac gttgcattgg gaaggttctc agtgacctca ccttgcatct cgatgacatc
     5761 ttccttcgtc atgcgctcac ctgccgggca gacccgcccc cttgaaattc gcctttttca
     5821 gcagactctc gtattggtgc gacatcacga acgcctggac ctgggccatg aagtccatcg
     5881 tgaccaccac gaggatcagc agcgaggtgc cgccgaaata gaacggtacg ttccacttca
     5941 ggatcaggaa ctccggcagc aggcagacga gggtgatgta gatcgccccg gccagcgtca
     6001 gccgcatgag aatcttgtcg atatagcgcg cggtttggtc gccgggacgg atcccgggca
     6061 caaaggcccc gctcttcttc aggttgtccg ccgtttcgcg ggcattgaac accagcgccg
     6121 tgtagaagaa gcagaagaac acgatcgcca ccgcatacaa catcacatag atcggctgac
     6181 cgggagacag cgtggatgcg atgtcgcgca accagtacat gccctccgag gaaccgaacc
     6241 actgccccag cgtcgccgga aacaggatga tgctcgatgc gaagatcggc gggatcacgc
     6301 ccgacatgtt caacttcagc ggcagatgcg agctttgccc cccatacacc ttgttcccga
     6361 cttgccgctt cgcgtagttc accaggatct tgcgttggcc acgctcgacg aacacgacga
     6421 aagccgtcac cagcaccacg aggatgcaga tgaagagcgc ggtgaaggga tgcatggcac
     6481 cggtccgcac cagttcgaac aagccaccga tcgcagccgg caagccggca atgatgcctg
     6541 cgaagatgat catcgagatg ccgttgccga tgcctcgctc ggtgatctgc tcacccagcc
     6601 acatcaggaa catcgtcccg gtcaccaagg tcgccaccgt cacgaagcgg aacatcagtc
     6661 ccggctcgag caccaggcca gcctgtcctt ccagcgcgat cgcgatcccc agcgcctgcg
     6721 caagcgcgag cacaagggtt ccatagcgtg tgtattgcgt gatcttgcgt cgccccgcct
     6781 cgccttcctt ctttagcgcc tccagcgaag gcaccgccac ggtcatcaac tgcatgatga
     6841 tggacgcaga gatgtagggc atgatgccca gcgcaaagat ggtgaagcgc gacagcgcac
     6901 cgcccgagaa caggttgaac acgcccagga tgccaccctg ctgggactgg aacagctcgg
     6961 ccagcccctg cgggtcgatt cccggaaccg ggatatgcgc accaatccga taaacgatca
     7021 gcgcgcccac caggaacagc aggcgtcgct tgagatcacc gaaccgtccg gggcttccca
     7081 gcgtggcagc aggattcgcc acaaccgttt ccttttgtta ctcggccgcc acggagccgc
     7141 cagcggcttc gatcgccgcc ttcgcaccct gggtggcgcc gacgccgcgc agaaccacct
     7201 tgcggctgat cgaaccggac agcacgacct tcgccgacag cgcgtcggcg gcgatcaccc
     7261 cggcctgctt cagcgtcagc agatcgactt catcgaccgg cagcttgtcg agctcggaca
     7321 ggcgtacctc gacgttgcgg gctcgcgtca gcgacacgaa tccacgcttc ggcagacggc
     7381 gctgcagcgg catctgaccg ccctcgaagc cgaccttgtg aaagccaccg gcgcgggact
     7441 tctgaccctt gtggccgcgg ccgcaggtct tgcccaggcc gctgccgata ccacggccga
     7501 ctcggcgcgc ttccttcttc gaaccctcac cgggcttgat gctattcagg cgcatttcaa
     7561 ccctcgcact tgaccaggta ggacaccttg ttgatcatcc cgcgcacctc aggggtatcc
     7621 gccagctcga ccgaatggtt gagccgacgc agaccgaggc cgcgcacggt ggcgcggtgg
     7681 gactgcttgg tgccgatcac gctcttgacg agcgtcacct tgattttctt gtccgacatc
     7741 tcttacccca ggatctcttc gacggacttg ccgcgtttcg cagcgatttc cgccggggtg
     7801 ttgatcgcca tcaggccgtt gatcgtggca cggacgacgt tgtacggatt ggccgaaccg
     7861 atgcacttgc agatcacgtc ggtaacgccc atgacttcga acacggcgcg catcggaccg
     7921 ccggcgatga tgccggtacc ggccggagca ggctgcatga gcaccgaagc ggcaccatgc
     7981 ttgcccgtca cggtatgctg aagggtgccg ttcttgagcg agaccttgcg catcttgcga
     8041 cgcgcttcat ccatcgcctt ctgcaccgcc accggcactt cgcgggactt gcccttgccc
     8101 atgccgatcg caccgtcgcc gtcaccgacc acggtcagcg cggcgaagcc gaggatacga
     8161 ccgcccttga cgaccttggt cacgcgattg atcgcgacca tcttttcctt caggccgtca
     8221 tcgcgctcgt cggaggcttg cgcacgtttg gtttgctgct tggccatacg attcctcgct
     8281 tagaacttga gaccgccttc gcgggcggcc tcggccagcg ccttcacgcg gccgtgatac
     8341 aggaagcccg agcggtcgaa ggacacctgc tcgacgccgg cagccttggc gcgttcggca
     8401 atcagcttgc cgaccgcttc ggccgcggcc ttgttgccgc cgttggcgac ctgggcacgg
     8461 acgtccggct ccacggtgga agccgccgcg agcacgcggg aaccgcaacc ggagatgatc
     8521 tgggcgtaga tgtggcagtt ggaccggaac accgtcaggc gaaccgcctt gagctccgca
     8581 atcttggcgc gagttttgcg cgcacggcga agacgagttt ctttcttgtt catgacgaac
     8641 cgccttactt cttcttggtt tccttgagca ccaccacctc gtccgagtaa cggacgccct
     8701 tgcccttata gggctcggga gcgcggtagg cacgcacctc ggcagcgacc tggccgacct
     8761 tttgcttgtc ggaacccctg atcacgatct cggtctgggt cggcgtctcg accttcacgc
     8821 cttccggcat ctggtgaacc accgggtgag agaaaccgag cgacaggttg agcttgtcgc
     8881 cctgagcctg cgcgcgataa cccacgccga ccagggtgag cttgcgctcg aagcccttgg
     8941 tcacgcccgt caccatgtta ttcaccagag cgcgcacggt gccggacatg gcacgggcgt
     9001 tctcggcgcc ctcacgcgcc ttgcacacca ccgcgtcacc ttcgcggacc acgctgacct
     9061 cgccgctcag cggctggcgc agggtaccca gaggcccctt cactgcgatc tcggcgtcgg
     9121 aaatcatcac ttccacgccc tgcgggatcg ccaccggatt cttggctaca cgagacatcg
     9181 tatccccctt aggcgacgag gcagatgact tcgccaccga cgcggttcgc acgcgccgag
     9241 cgatcggtca tcacgccacg cggcgtggaa acgatagcca cgccgaggcc gttcatcacg
     9301 cgcggcagat cgtcgctacc cttgtagatg cgcaggccgg gacggctcac gcgctccagg
     9361 cgctcgatga ccggacgacc agcgtaatac ttgagggtga cgtcgagcga agccttgcca
     9421 gcctcgtcac ggacggcgaa gccgtcgatg taaccctcgt cctgcaacac cttggcgatc
     9481 gcaaccttca gcttggacga aggcatcgac accgaagcct tctgggcctg ctggccgttg
     9541 cggatgcgcg tcagcatgtc ggcgatcgga tcggacatac tcatcttcga attctcctta
     9601 ccagctcgcc ttggtcatgc caggcacttc gccgcggaaa gcgatctcgc gcagcttgtt
     9661 gcggcacaga ccgaacttgc ggaagacacc acgcgggcga ccggtcagcg cgcagcggtt
     9721 gcgctggcga gtcgggttag cattgcgcgg cagttgctgc agggccaggc gggcagccat
     9781 gcgttcttct tcggacagac tggtgtcctt gaccttcgcg accagagccg cgcgcttgga
     9841 ggcatacttc tccaccagca tcgcacgctt cgcttcgcgg ttgatcagag cgagtttcgc
     9901 catatcaccc tcaattcttg aacgggaact tgaacgcacc gaggagagca cgggcttcct
     9961 cgtcggtctt ggccgtcgtc gtgatgctga tgttcatccc gcgcagcgcg tcgatcttgt
    10021 cgtactcgat ctcggggaag atgatctgct ctttcacgcc gaggttgtag ttgccacgac
    10081 cgtcgaaacc cttggcagcg ataccgcgga agtcgcgaac gcgcggcagc gcgatcgtga
    10141 tgaggcgatc gaggaattcg aacatgcgcg gaccacgcag ggtcaccatg cagccgatcg
    10201 gatagccgtc acggatcttg aaacccgcga tcgacttgcg cgccttggtc gaaaccggct
    10261 tctgacccgc gatcttggtc atgtcgccaa tggcgtgctc gagaatcttc ttgtcgccga
    10321 ccgcctcacc gacacccatg ttgagggtga tcttggtgat gcgcggcact tccatgacgg
    10381 acttgtagcc gaactgctgc tgcagcgccg gcgcaacttc gtccttgtaa acctgttgca
    10441 agcgagccat cgtcactcct taggcgttca ccagctcgcc gttcgacttg aagaaacgaa
    10501 ccttgcggcc gtcttcaagc gtcttgatcc cgacacgatc acccttctgg gtggcgggat
    10561 tgaacagcgc gacgttcgac acatggatcg gcatctcctt ctcgacgatg ccacccactt
    10621 cacccttcag cggattcggg cgcacgtgct tcttgacccg gttcacgcct tcgaccacga
    10681 cgcgctcgtc atcgacgcgg cgcagcacgg tgccgcgacg accacgatcc ttgcccgtca
    10741 gcaccaaaac ttcgtcaccc ttgcggatct tgttcatctc tggactcctc agagcacttc
    10801 gggcgccagc gagacgatct tcatgaaccg ctcggtgcgc agctctcgcg tgaccggccc
    10861 gaagatacgg gtgccgatcg gctcgagctt gttgttcaga agcacggccg cattgctgtc
    10921 gaacttgatc agcgaaccgt cgggacgacg aacgcccttg gcggtgcgaa cgacgaccgc
    10981 gttgtagacg tcgcctttct tggcacgacc acgcggagcg gcatccttca cggtcacctt
    11041 gatgatgtcg ccaacgccgg catagcgacg cttagaacca cccaggacct tgatgcacat
    11101 caccgagcgc gcgccggagt tatcggccac gtccagcttg gactgcattt gaatcatgaa
    11161 agtatctcca acttatcccg catgtgcgga cagtcttgga acccgtcagg gtaggatgcc
    11221 ccgagcgagc cccggagcaa agaggcaaaa ttataacagc ctggcacctg ggtgccaagc
    11281 tgtttgttta ctgctcgctg cttccaactc cgatcagatg atcgtcgcct tctcgagcac
    11341 cttggccaca cgccaggtct tggtgcgcga gatcggacgg cattcctcga tctcgaccag
    11401 gtcgccctgg gccgcgacgc cgtcctcgac gtgcgcgtgg tacttcgccg aacgggtgat
    11461 gatcttgccg tagagcgggt gcttgacccg gcgctcgacc agaacggtca ccgtcttctg
    11521 cattttgtca ctcaccacac gcccaaccag cgcgcggcga accttctcga cgttctcgct
    11581 cattgctgac ccgccttctc acgcaggatg gtgcgcacgc gcgcgatgtc gcggcggacc
    11641 ttcccgatct gactcgtgtt gccgagttgc tgcgtggcgt gctgcatgcg cagcgaaaac
    11701 tgggccttca gcagctcgat cagttccttg ttcaactcgt cggcgctctt ggcgcggagt
    11761 tccgttgcct tcatgttcaa cccccaatca gtcgggtgac gaacacggtc tcgatcggca
    11821 gcttggcagc cgcaaggcgg aacgcctcgc gcgccagggc ctcgtcaacc ccgtccatct
    11881 cgtacagcac cttgcccggc tggatctcgg caacccaata ttccgggttg cccttgccgt
    11941 tacccatgcg gacctcggcc ggcttcttcg aaatcggctt gtccgggaaa atccggatcc
    12001 agatgcggcc gccacgcttg atatgacggg tcatcgcacg acgggcggac tcgatctggc
    12061 gggcagtcag ccgaccgcga ccgatggcct tcagaccata ctcgccgaac gacaccttgg
    12121 cgccgcgcgt ggcgacgccg gtgttgcggc ccttctgctc cttccgatac ttccttctag
    12181 tcggctgcag catcattcgc tcctgctttc ttcttgccgg ccttcgccac gacgggcggg
    12241 acgacgaccc ggacggccct cgccgtcacg cggggcgcca cgacgaccgc gacggttgtc
    12301 ggcttcgggc tccgcagcca cgggctgctc gttgcgcccc agcatctcgc ccttgaacac
    12361 ccagaccttg atcccgatga caccataggt cgtcttggcc tcggagacac cgtagtcgat
    12421 gttggcgcgc agggtatgca gcggcacacg cccttcgcga taccactcgg tgcgggcgat
    12481 ctctgcgccg ttgaggcggc cggagctcat gatcttgatg ccctgggcac cgagacgcat
    12541 ggcgttctgc atcgcacgct tcatggcgcg
//
LOCUS       DAOQNN010000044        12543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2185_length_12543_cov_10.472534, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000044 DAOQNN010000000
VERSION     DAOQNN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12543
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2185_length_12543_cov_10.472534"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..660)
                     /locus_tag="PKV97_04540"
     CDS             complement(106..660)
                     /locus_tag="PKV97_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNB05177.1"
                     /translation="MSIYALGDRRPSFGEGSWIAHNATVIGSVTAGRNVNIWYNVVVR
                     GDNDPITIGDDTNIQDGSVLHNDDGIPLTIGTHVTVGHMAMLHGCTIGDGSLIGINAV
                     ILNNAVVGKQCIVGANTLIPEGKVIPDRSLVVGSPGRVIRTLSDTEVENLVVNAHNYV
                     DNARLYADRLCALEPSSVIAKPEQ"
     gene            848..1693
                     /gene="rlmJ"
                     /locus_tag="PKV97_04545"
     CDS             848..1693
                     /gene="rlmJ"
                     /locus_tag="PKV97_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HNB05178.1"
                     /translation="MLSYRHAFHAGNHADVLKHLVLIELLDYYGRKDKPWYYIDTHAG
                     GGCYALDSEQAGKTAEAADGIGRLWGHEDLPPAVAAYVAAVGQFNPQGRLLFYPGSPA
                     LAMTRARAQDRLRLFELHPADFESLQRTFASEQERVQVRRADGFAALKSLLPPPSRRA
                     VVLIDPPYEVKDDYRSVVDTVRDAMRRFPTGTYMVWYPMLARAEARQLPERLAALGAE
                     SWLDVRLAVRDAPRDGIGMFGSGLYVINPPWVLPQRLEAALPWLVEHLGVDGGAGFDL
                     EHHIE"
     gene            1801..2118
                     /locus_tag="PKV97_04550"
     CDS             1801..2118
                     /locus_tag="PKV97_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNB05179.1"
                     /translation="MRQFAAERAWEPFHTPKNLAMALAGEAGEVIEHFQWLTAEQSMA
                     LPPATRDEVALELADVLLYLVRLGDVLGVDLADAARRKMRINAERYPIERAHGRADKH
                     DRL"
     gene            2217..3797
                     /locus_tag="PKV97_04555"
     CDS             2217..3797
                     /locus_tag="PKV97_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNB05180.1"
                     /translation="MDLQHISDIEKAAHQGRGEVFRLGMGVLFIVGVMFYSALSGTGE
                     GSMTLVMAAMIGGYMAMNIGANDVANNVGPAVGSKALTLAGALVIAAIFEAAGALIAG
                     GEVIGTIRSGIIDPDLIKDSDTFVWIMLAALLAGALWLNLATAVGAPVSTTHSIVGAV
                     LGSGMAAAGPSIVDWSTMGEIAASWVISPLLGGIFAAAFLYLVKRSITYQVDMAAAAR
                     RTVPVLVGLMAWTFVSYLILKGLNRVWKVDFLPAVLYGLIFGALVFVLVRKALHGSSG
                     IVANTKASVNRLFTVPLIFAAALLSFAHGSNDVANAVGPLAAIVDVVSSGGVVHKDAA
                     IPTWVMMVGAIGISLGLALFGPKVIRTVGSEITELDQMRAYCIAMAATLTVIVASQLG
                     LPVSSTHIAVGGVFGVGFLREYLKSNYDRMVAEIKAHHPEGDLAAIEAFIARFEKASI
                     EEKGQMLADLKQRSKNADDPAHFSKGERKGLKKVYRRELVKRSQLMRIAAAWVITVPA
                     SALMAAVLFFMIRGMMLP"
     gene            3838..4197
                     /locus_tag="PKV97_04560"
     CDS             3838..4197
                     /locus_tag="PKV97_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HNB05181.1"
                     /translation="MSFVELLVLLALGAVVWFWFDSLKAREIGIEAARRACTREGVQF
                     LDETVAGQGLRLARDDNGRVLLRRGYAFEYSVGGDDRNAGSVVLEGREVVLVDVSAHR
                     LARRAVVIDLHPRDPSA"
     gene            complement(4194..4847)
                     /gene="folE"
                     /locus_tag="PKV97_04565"
     CDS             complement(4194..4847)
                     /gene="folE"
                     /locus_tag="PKV97_04565"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNB05182.1"
                     /translation="MSPPAHDPCGHDHPHAPRPAGVGIPRSGAFDAPAFEKAVGDLLR
                     ACGIAPDNAHTGRTAQRVRELWQRRLLGGYELDPAEVLGDGFEDARRDMVVIRGIAVH
                     GVCPHHLVPFRGVAHVAYLPGGRLHGFGRIARLVDAIGHRFTYQEWMTRDIADALMRH
                     GHAAGAACVIEAEQLCLLLGEDRRGDERVITQAFAGEFERSEQARNEFLRAIEERRR"
     gene            complement(4907..5503)
                     /locus_tag="PKV97_04570"
     CDS             complement(4907..5503)
                     /locus_tag="PKV97_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05183.1"
                     /translation="MIDMDAARAMLGRQAILLPDAVPSHPLLDGKTEIGRGEYSIVID
                     KGDGEHVYKIVSSPADYFLYTADDRPRGPHFPIVFADHGVIGRAQSGYPFHLIEMERL
                     YPLAEGSPAAELSRRLIECYWSACAQWSRLGADMGRIALYHLTVEPMKVGDSLQEALR
                     ALSDFVEEYQVLPDILNANNLMMRADGTLVFSDPVFIA"
     gene            complement(5518..6297)
                     /gene="fabI"
                     /locus_tag="PKV97_04575"
     CDS             complement(5518..6297)
                     /gene="fabI"
                     /locus_tag="PKV97_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HNB05184.1"
                     /translation="MTPIVNLEGKVGLITGIANEKSISTGVAEACAQAGATLIITYQN
                     EKTRAFIEPVIHRLGVEDVHLLDVTRPETMDAAFGAIEARHGKLDFAIHSMAFAKRDD
                     LHGRVVDTSADGFALAMDVSTHSFVRLAKKAEPLLEKAGGGALVTMTYLGSEKVIPNY
                     GVMGLCKAALEAATRYMAAELGAKGIRVNAVSPGPLMTRAASGIAGFDGLMAAAVERS
                     PMHALVTPEDIGALTAFLVSDAGRLISGGVHFVDAGYNIMA"
     gene            6485..6994
                     /locus_tag="PKV97_04580"
     CDS             6485..6994
                     /locus_tag="PKV97_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HNB05185.1"
                     /translation="MTRNAYIFMDFDGVTHPWGEVEDFRCLVHIEAVVREFPEARIVI
                     ASDWRMLFSLPKLVARFSEDIRPQIAGATPHLLPKTGAELHGLREREAMAWLRQHGAD
                     PGEAAWCALDDAPGNWLSRSRLVLTDFKRGFTSEDAVALRRMLQGFRDGVRNQVGQVA
                     HSLWARDSA"
     gene            7009..7779
                     /locus_tag="PKV97_04585"
     CDS             7009..7779
                     /locus_tag="PKV97_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05186.1"
                     /translation="MNPAEQTLPPDPLAHRVDASTQRQAARLAEESFARLFRLSVAEG
                     DAARLKGVEQLRVDLADWVSAAADPEAQALRLALLLSGMDQWGMAWSRAFGLVAIPAL
                     TELIGALRTGLDETAEARFLRHFEGIAAVEENAIDFKVELRRGLHLALWHASIATEHR
                     DEAMRLAGELGSQLLALARSMPVAGWRLVADALAFIQIRCLAEGLAAEGVAQEATQAL
                     FGALARELPAPQRDLVMAHSARAVMAWQQAGRASPQVH"
     gene            7779..8315
                     /locus_tag="PKV97_04590"
     CDS             7779..8315
                     /locus_tag="PKV97_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNB05187.1"
                     /translation="MALKATVFKADIQVADMDRHHYADYSLTLARHPSETDERMMVRL
                     LAFALFADPALAFGKGLCVDDEPDLWQKDLTGAIERWIDVGQPDDKWVRKACGRAREV
                     VVLAYGRALEVWWSGVRGRLERHGNLKVFELEREASAELGRLAERTMRLQFTIQDGHV
                     LVTNGRETVSLEPRKLTA"
     gene            complement(8364..9248)
                     /locus_tag="PKV97_04595"
     CDS             complement(8364..9248)
                     /locus_tag="PKV97_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB05188.1"
                     /translation="MKLQSWTHHTSAGFTLRGHHSPPSGRPVLHFLHGNGLSSLTYAP
                     MLAHLSAHFDLFLSDVQGHGDSDHGSRFRGWNHAAEMAAEAWLAHAPRFGTVPVHAAG
                     HSFGSVLTCLMLAHHPHAFSRGVLLDPVLFTPAMIGMMALSDLVGLASRNKLAAKART
                     RRHHWPDRAAAHAGLHGRGVFRGWSEDSFQAYIDHALRDVPGGGVELKCRPTREAEIF
                     ASYPRRLWSSLARIEQPVLALHGRDTFAFARRSVTRWAAGKANVQCVQVEGGHCFMLE
                     HPQATAERVAAFLRDSPR"
     gene            complement(9310..>9840)
                     /locus_tag="PKV97_04600"
     CDS             complement(9310..>9840)
                     /locus_tag="PKV97_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB05189.1"
                     /translation="PLMQAGFVASVRSALERHPDIPPERLELEILESTALDDMVLAMD
                     VLEACHRLGVRIALDDFGTGYASLRHFRQLPFDRLKIDRSFVLDMLEDGEARAIVGAV
                     VQLARTFGREVIAEGVETPAHAAALLALGCRLGQGFGIARPMPVEHIPGWVSWQTLAP
                     CADDAACASSGRCPPI"
     assembly_gap    9841..9940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9941..12186)
                     /locus_tag="PKV97_04605"
     CDS             complement(<9941..12186)
                     /locus_tag="PKV97_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNB05190.1"
                     /translation="MLIRRLLRTVVLLVCLSLGSTALHARTELDFGVYSARPKALVEA
                     SWQPFVDYLNQHLYGVEVRLHALDDAGLQRALAAGEIDLLLTNPVHFIELRASNPLSG
                     AIATVVADAGGQALSEFGGVILVRAGDASLKTLADLRGRRVGTTHANFLATYPAQALE
                     MKAAGIDPGSLRMFTLQQTQDSVVEAVLDGRVDAGFVRTGLFEALVAEGRDLSGLRVL
                     HPLQHPGFPLRSSTRLYPEWPLVALRQADPALMRKVAALALGLETAAPAARAAGIHGF
                     TIPADYARVEEMLRELRLPPFDATPPFTWNDVWARYHETIFVLGLATLAIVLLLVRLA
                     QRNLALSQANAAARRLAAEIELDRHHLRNVIEATQAGSWEWNLETGEWKMDARWAAML
                     GLAAADCDGEGRACWRKRVHPADLPEAERELDRHIRGETAFYEHDLRLRHRDGRWIWV
                     YDRALAVRRDADGRALLLTGAQIDIDRRKENEERLRLAASVFSSSYEAILITDVDNRI
                     VDVNPAFCRITGYSREESLGRDPGMLASGRQGREFYRAMWQAIEEHDHWQGELWNRRK
                     DGSEFAEVLSISRVRDARGRVIHHLAMFSDISRLKKHEEELNRIAYFDPLTGAPNRRL
                     LDDRLRQAIAHSRRTGKPLAVCVIDLDGFKPVNDQLGHEAGDQVLIGIVDRLGAILRA
                     SDTVARLGGDEFVLLLEDIEGERVLERVLEAIRTPIALASQQVCVSASIGVTLFPEDD
                     ADPDTLLR"
BASE COUNT         1814 a         4146 c         4525 g         1958 t
ORIGIN      
        1 cctcggccgc gagctccacg gtgggcgggg cgggagtgtc ggcggcaagc gccggcgccg
       61 actgcagcgc cgcgagcgcg gcgagggtgg cgagcgcggt gcgcctcact gttccggctt
      121 cgcgatcacg ctggacggct ccagcgcgca caggcggtcg gcgtagaggc gggcgttgtc
      181 cacgtagttg tgggcgttga ccaccaggtt ctcgacctcg gtgtcgctca gcgtgcggat
      241 cacccggcct ggagaaccga ccaccagcga gcggtcgggg atcaccttgc cctcggggat
      301 cagggtgttg gcgccgacga tgcactgctt gccgaccacc gcgttgttga ggatcaccgc
      361 gttgatgccg atcaggctgc cgtcgccgat cgtgcagccg tgcagcatcg ccatgtggcc
      421 cacggtgacg tgcgtgccga tcgtcagcgg gatgccgtcg tcgttgtgca ggacagagcc
      481 gtcctggatg ttggtatcgt cgccgatcgt gatcggatcg ttgtcgcccc gtaccacgac
      541 gttgtaccag atgttgacgt tgcggcccgc agtgaccgat ccgatgaccg tcgcgttgtg
      601 ggcgatccag cttccctcgc cgaagctcgg ccggcggtcg cccagggcgt agatgctcat
      661 tttcgttctc gctgtcgatg tctcgcgccg atgccgcgca gcgccagggg ggcaggcatc
      721 ataacaagcg cctcgcggcc tgcaaacagc gcccgtgccg tgaatgtcgc cgatccggcg
      781 ggtttttcgc gattttgagc cccctgccag cgggtgatag gatgcgcccg cccttcagga
      841 agaccacatg ctcagttacc gtcacgcctt tcacgccggc aatcacgccg atgtcctcaa
      901 gcacctcgtc ctgatcgagc tgctcgacta ctacggtcgc aaggacaagc cctggtacta
      961 catcgacacc cacgcggggg gcggctgcta tgcgctcgac agcgagcagg cgggcaagac
     1021 cgccgaggcg gcggacggca tcggccggct gtgggggcac gaggacctgc cgccagcagt
     1081 cgccgcctat gtcgcggcgg tcgggcagtt caatccgcag ggcaggctgt tgttctaccc
     1141 gggctcgccg gcgctggcca tgacgcgtgc gcgcgcccag gatcgcctgc gcctgttcga
     1201 gctgcatccg gccgacttcg aatccctgca gcgcaccttc gccagcgaac aggagcgcgt
     1261 gcaggtgcgc cgcgccgacg gcttcgccgc gctcaagagc ctgctgccgc cgccctcgcg
     1321 gcgggcggtg gtgctgatcg atccgcccta cgaggtcaag gacgactacc gcagcgtggt
     1381 ggacacggtg cgtgacgcga tgcggcgctt tcccaccggg acctacatgg tctggtatcc
     1441 gatgctcgcc cgcgccgagg cccgccagtt gcccgagcgc ctcgccgcgc tcggcgccga
     1501 gagctggctg gacgtgcgcc ttgcggtgcg cgacgccccg cgcgacggca tcgggatgtt
     1561 cggcagcggc ctttacgtga tcaaccctcc ctgggtgttg ccgcagcggc tcgaggcggc
     1621 gctgccctgg ctcgtcgagc acctgggggt cgatggcggt gcgggcttcg acctcgagca
     1681 ccacatcgaa tgagcccgtc ccggaatgcc gagggtgggc ccgccggccc gacggtcgcg
     1741 gagggcgctc cgagtgctgc aggcccgtcg cccgacgcgc tccgcgagct gcgcgacgcg
     1801 atgcggcaat tcgccgccga gcgcgcctgg gagcccttcc atacgcccaa gaacctcgcg
     1861 atggcgctcg ccggcgaggc gggcgaggtg atcgagcatt tccagtggct caccgccgag
     1921 cagtcgatgg cgctgccgcc ggcgacgcgg gacgaggtcg ctctcgagct cgccgacgtg
     1981 ctgctgtacc tcgtgcgcct cggcgacgtg ctcggtgtcg acctcgccga tgccgcacgg
     2041 cgaaagatgc gcatcaatgc ggagcgctac cccatcgagc gcgcgcacgg ccgcgcggac
     2101 aagcacgacc gtctgtgagg gtgcaagcgc ggcgttgcgg cggagtcttc attcacgttg
     2161 ggccgcggca agatttctga aacaatatcg tctttcttgt aacacggaat tccatcgtgg
     2221 acttgcagca catcagcgac atcgagaagg cagcccatca gggtcggggc gaggtcttcc
     2281 gcctcggcat gggcgtgctc ttcatcgtcg gggtcatgtt ctactcggcg ctcagcggca
     2341 cgggcgaggg cagcatgacc ctggtcatgg cggcgatgat cggcggctac atggcgatga
     2401 acatcggcgc caacgacgtc gccaacaacg tcggtccggc ggtgggctcc aaggcgctca
     2461 ccctcgcagg tgcgctcgtc atcgcggcga tcttcgaggc cgccggtgcg ctgatcgccg
     2521 gcggcgaggt gatcggcacc atccgcagcg gcatcatcga tcccgacctc atcaaggact
     2581 ccgacacctt cgtgtggatc atgctggccg cgctgctcgc cggcgccttg tggctgaacc
     2641 tggcgaccgc ggtcggggcg ccggtgtcga ccacgcactc gatcgtgggc gcggtgctcg
     2701 gctccggcat ggccgccgcc ggcccctcga tcgtcgattg gtccaccatg ggcgagatcg
     2761 cggcgagctg ggtgatctcg ccgctgctcg gcgggatctt cgccgccgcc ttcctgtatc
     2821 tcgtcaagcg cagcatcacc taccaggtgg acatggccgc ggccgcacgc cgcaccgtgc
     2881 cggtactggt cggcctgatg gcgtggacct tcgtcagcta cctgatcctg aaggggctca
     2941 accgggtctg gaaggtggac ttccttcccg ccgtgctgta cggcctgatt ttcggcgcgc
     3001 tggtgttcgt cctggtgcgc aaggccctgc acggcagcag cggcatcgtc gccaacacca
     3061 aggcgagcgt gaatcggctc ttcaccgtgc cgctgatctt cgccgccgcg ctgctcagct
     3121 tcgcgcacgg ctccaacgac gtcgccaacg cggtcggccc gctggccgcg atcgtcgatg
     3181 tggtcagttc cggcggcgtg gtgcacaagg acgcggcgat ccccacctgg gtcatgatgg
     3241 tcggcgcgat cgggatctcg ctcgggctgg cgttgttcgg gccgaaggtg atccgcacgg
     3301 tcggttccga gatcaccgag ctcgaccaga tgcgcgccta ttgcatcgcg atggcggcga
     3361 ccttgaccgt gatcgtcgcc agccagctcg gcctgccggt gagctccacg cacatcgcgg
     3421 tcggcggggt gttcggcgtc ggtttcctgc gcgaatacct caagagcaac tacgaccgca
     3481 tggtggccga gatcaaggcc caccaccccg agggcgacct cgccgcgatc gaggccttca
     3541 tcgcgcgttt cgagaaggcc tcgatcgagg agaaggggca gatgctcgcc gacctcaagc
     3601 agcgctccaa gaacgcggac gatccggcgc acttctccaa gggcgagcgc aagggactca
     3661 agaaggtcta tcgccgcgag ctggtcaagc gctcgcagct gatgcgcatc gccgccgcct
     3721 gggtcatcac cgtgcccgct tctgcgctga tggccgcagt gctgttcttc atgatccgcg
     3781 ggatgatgct gccctgatca ccctgttcgt catgatcgcg atgccggggg ccgcggcgtg
     3841 agcttcgtcg agctgctcgt cctgctcgcc ctcggggccg tggtgtggtt ctggttcgac
     3901 agcctgaagg cgcgcgagat cggcatcgag gccgcgcgcc gcgcctgcac gcgcgagggc
     3961 gtgcagttcc tcgacgagac cgtggcgggg cagggcctgc gcctggcgcg cgacgacaac
     4021 ggtcgcgtgc tgctgcggcg gggctatgcc tttgaatact cggtgggcgg cgacgaccgc
     4081 aatgccggca gcgtggtgct cgaaggccgc gaggtggtgc tggtggacgt ctccgcgcat
     4141 cggctcgcac gtcgcgcggt ggtcatcgac ctgcacccgc gcgacccgtc ggcctagcgc
     4201 cggcgctcct cgatcgcgcg caggaactcg ttgcgcgcct gctccgaacg ttcgaactcg
     4261 ccggcgaagg cttgggtgat cacgcgttcg tcgccacggc ggtcctcgcc gagcagcagg
     4321 cagagctgtt cggcctcgat cacgcaggcc gcgcccgcgg cgtggccgtg gcgcatcagc
     4381 gcgtcggcga tgtcgcgcgt catccactcc tggtaggtga agcgatggcc gatcgcgtcc
     4441 accaggcggg cgatgcggcc aaagccgtgc aggcggccgc ctggcaggta ggcgacgtgg
     4501 gcgacgccac ggaaagggac gaggtgatgc ggacacacgc catggaccgc gatgccgcga
     4561 atcaccacca tgtcgcggcg cgcatcctcg aagccgtcac cgagcacctc ggcggggtcg
     4621 agctcgtagc ccccgagcaa gcgccgctgc cagagctcgc gcacgcgctg cgcggtgcgt
     4681 ccggtatggg cgttgtcggg ggcgatgccg cacgcacgca gcaggtcgcc gacggccttc
     4741 tcgaaggcgg gcgcgtcgaa ggcgccgctg cgcggaatgc cgacgccggc ggggcgggga
     4801 gcgtgggggt ggtcatgacc gcaggggtcg tgcgcaggag ggctcatggt ggcgtccggt
     4861 gcggtgccct gcggcacccg agccggcatg atacccgcaa gcccgttcag gcgatgaaca
     4921 ccgggtcgga gaacaccagc gtgccgtcgg cgcgcatcat caggttgttg gcgttgagga
     4981 tgtcgggcag gacctgatat tcctcgacga agtcggacag ggcccgcaag gcttcctgca
     5041 ggctgtcgcc gaccttcatc ggctcgaccg tgaggtggta gagcgcgatc cgccccatgt
     5101 ccgcaccgag ccggctccac tgcgcgcacg ccgaccagta gcactcgatc aggcgacgcg
     5161 agagttcggc cgcgggggag ccctcggcga gcggatagag gcgctccatc tcgatcagat
     5221 ggaagggata gcccgactgt gcgcggccga tcaccccatg atcggcgaat acgatgggga
     5281 aatgcgggcc gcgcgggcgg tcgtcggcgg tgtagaggaa gtagtcggcg ggggagctga
     5341 cgatcttgta gacatgctcc ccgtcgccct tgtcgatcac gatcgagtac tcgccgcgac
     5401 cgatctcggt cttgccgtcg agcagcggat gcgacggcac ggcgtcgggc agcaggatcg
     5461 cctgccgtcc gagcatcgcg cgcgccgcat ccatgtcgat catgatctcc tcgctgctca
     5521 cgccatgatg ttatagccgg cgtcgacgaa atgcaccccg ccgctgatca gtcggccggc
     5581 gtccgagacc aggaaggcgg tcagcgcgcc gatgtcctcg ggggtgacca gtgcatgcat
     5641 cggcgagcgc tcgaccgcgg ccgccatgag gccgtcgaag cccgcgatcc cggaggcggc
     5701 gcgcgtcatc agcgggccgg gggagaccgc attgacgcgg atgcccttgg cgccgagctc
     5761 ggccgccatg tagcgggtgg cggcctcgag cgcggccttg cacaggccca tcacgccgta
     5821 gttggggatg accttctcgg agccgaggta ggtcatcgtc accagggcac caccgccggc
     5881 cttctcgagc agcggctcgg ccttcttggc caggcgcacg aaggagtggg tggacacgtc
     5941 catcgccagc gcgaagccgt cggccgaggt gtcgaccacg cggccgtgca ggtcgtcgcg
     6001 cttggcgaag gccatgctgt ggatggcgaa gtcgagcttg ccgtggcgcg cctcgatcgc
     6061 gccaaaggcg gcgtccatgg tttcggggcg ggtgacgtcg agcagatgaa cgtcctcgac
     6121 gccgaggcgg tggatcaccg gctcgatgaa ggcgcgcgtc ttctcgttct gataggtgat
     6181 gatcagcgtc gcgccggcct gggcgcaggc ctcggcgacg ccggtggaga tcgacttctc
     6241 gttggcgatg ccggtgatga ggccgacctt gccctccaga ttgacgatgg gggtcatgat
     6301 ggattccttt tctcgggtga gtgttgttgc ggcgcaacgg cagcgagcat aagccggcct
     6361 gtgcgacgcg aatgtgatgg ttcgtgaaat cggatacgcg gtggtatcgg tgccggtaca
     6421 gtatattgcg cattgcagca tccacaaatt gccggccgcg gcattcttcg ccggagcagg
     6481 gaacatgaca cgcaacgcat acatcttcat ggacttcgac ggcgtcaccc acccctgggg
     6541 cgaggtcgag gatttccgtt gtctcgtgca catcgaggcg gtggtgaggg agttccccga
     6601 agcgcgcatc gtgatcgcct ccgactggcg catgctgttc tcgctgccca agctggtcgc
     6661 tcgcttttcc gaggacatcc gcccgcagat cgccggcgcc acgccgcacc tgctgcccaa
     6721 gacgggcgcc gagctgcacg gcctgcgcga gcgcgaggcg atggcctggc tgcgccagca
     6781 cggcgccgat ccgggcgagg cggcgtggtg cgcgctcgac gacgcgccgg gcaactggct
     6841 gtcgcgttcg cgactggtgc tgaccgactt caagcgtggc ttcacctccg aggatgcggt
     6901 cgccctgcgc cgcatgctgc agggcttccg cgacggggtg cgcaaccagg tcggccaggt
     6961 cgcgcactcg ctgtgggcgc gcgacagcgc ctgatggggg gagacgccat gaaccccgcc
     7021 gagcagaccc tgccgcccga cccgcttgcg caccgagtcg acgcctcgac ccaacgccag
     7081 gcggcgcgct tggccgaaga gagcttcgcg cgcctgttcc ggctcagcgt cgccgaaggc
     7141 gatgctgcgc ggctgaaggg cgtggagcag ttgcgcgtcg acctcgccga ctgggtgtcc
     7201 gcggcggccg accccgaggc acaggcgctg cgcctcgcct tgctgctgtc cgggatggac
     7261 cagtggggga tggcctggag ccgggccttc ggcctggtgg cgatccccgc gctgaccgag
     7321 ctcatcggtg cgctgcgcac tggtctggat gaaaccgccg aggcgcgctt cctgcgccac
     7381 ttcgaaggga tcgccgccgt cgaggagaat gccatcgact tcaaggtcga gctgcgccgt
     7441 gggctgcacc tggcgctgtg gcatgcctcg atcgccaccg agcatcgcga cgaggccatg
     7501 cgcctcgccg gcgagctcgg cagccagctg ctcgcgctcg cgcgcagcat gccggtggcg
     7561 ggctggcggc tggtggccga cgcgctcgcc ttcatccaga tccgctgcct ggccgaaggg
     7621 ctcgctgccg agggcgtggc ccaggaggcc acacaggcct tgttcggcgc cttggcgcgc
     7681 gagctgccgg cgccgcagcg cgacctcgtg atggcgcatt ccgcgcgcgc ggtgatggcc
     7741 tggcagcagg ccggccgcgc ttcgccgcag gtgcactgat ggcgctcaag gccacggtct
     7801 tcaaggccga catccaggtc gccgacatgg atcggcacca ctacgccgac tattcgctca
     7861 ccctcgcgcg tcacccttcc gagaccgacg agcgcatgat ggtgcgcctg ctcgccttcg
     7921 cgctcttcgc cgacccggcg ctcgccttcg gcaaggggct atgcgtcgat gacgagcccg
     7981 acctgtggca gaaggacctc accggcgcca tcgagcgctg gatcgacgtc ggccagcccg
     8041 atgacaagtg ggtgcgcaag gcctgcgggc gcgcgcgcga ggtggtggtg ctggcctacg
     8101 ggcgggcgct ggaggtgtgg tggagcgggg tgcgcggccg cctcgagcgc cacggcaacc
     8161 tgaaggtgtt cgagctcgaa cgcgaggcca gcgccgaact cggccgcctc gccgagcgca
     8221 cgatgcgcct gcaattcacc atccaggacg gccacgtgtt ggtaaccaac gggcgcgaga
     8281 ccgtgtcgct cgagccgcgc aagctcactg cctgaacccc ggtgcgcggc gcgcgacagc
     8341 ccgcggcgcg gccgcccggg ccctcagcgc gggctgtcgc gcaggaatgc ggccaccctt
     8401 tcggcggtcg cctgcggatg ctcgagcatg aagcagtgtc cgccctccac ctgcacgcat
     8461 tgcacgttgg ccttgcctgc cgcccagcgc gtgaccgagc gccgcgcgaa ggcgaaggtg
     8521 tcgcgcccgt gcagggcgag caccggctgc tcgatgcggg caagcgagga ccacagccgg
     8581 cgcgggtagg acgcgaagat ctcggcctcg cgggtgggcc ggcacttgag ctccacgcca
     8641 ccgcccggca cgtcgcgcag cgcatggtcg atataggcct ggaaggagtc ctcgctccag
     8701 ccgcggaaca ccccgcgccc gtgcagcccc gcatgcgcgg cagcgcggtc cggccagtgg
     8761 tggcgtcgcg tgcgtgcctt ggcggcgagc ttgttgcgcg aggccagccc gacgaggtcg
     8821 gagagcgcca tcatgccgat catcgccggg gtgaacagca ccgggtcgag cagcacacca
     8881 cggctgaagg cgtgcggatg atgcgccagc atcaggcagg tgagcacgct gccgaagctg
     8941 tggccggcgg catgcacggg taccgtgccg aagcgcggtg cgtgcgcgag ccaggcctcg
     9001 gcggccatct cggcagcatg gttccagccg cggaagcggc tgccgtggtc gctgtcgcca
     9061 tgaccctgca cgtcggacag gaagaggtcg aaatgcgcgc tcaggtgcgc gagcatgggc
     9121 gcataggtga gcgagctgag cccgttgcca tggaggaaat gcagcaccgg gcgaccgctc
     9181 ggcgggctgt ggtggccgcg cagcgtgaag ccggcggagg tgtgatgcgt ccagctttgg
     9241 agcttcatga ttcggcgtat gccgcggctg cgagcgcggt cgtggcggag gtcggattgt
     9301 agggccagat cagattggcg ggcagcggcc ggagctcgcg cacgccgcat cgtcggcgca
     9361 gggtgcgaga gtctgccatg aaacccagcc ggggatgtgc tcgaccggca tcggccgggc
     9421 gatgccgaag ccctggccga gcctgcagcc gagggcaagc agggccgctg cgtgggcggg
     9481 cgtctccacg ccttcggcga tcacctcgcg gccgaaggtg cgcgcgagct gcaccacggc
     9541 gccgacgatc gcgcgcgcct cgccgtcctc cagcatgtcg agcacgaagc tgcggtcgat
     9601 cttgaggcgg tcgaagggca gctggcggaa gtggcgcagc gaggcgtagc cggtgccgaa
     9661 gtcgtcgagc gcgatgcgta cgccgaggcg atggcaggcc tcgagcacgt ccatcgccag
     9721 caccatgtcg tcgagcgcag tggattcgag gatctcgagc tccaggcgct cgggcgggat
     9781 gtcggggtgg cgctccagcg cgctgcgcac gctggcgacg aagcccgcct gcatcagcgg
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tggcgcagca gggtgtcggg
     9961 gtcggcgtcg tcctcgggga agagggtgac gccgatgctc gcggacacgc atacctgctg
    10021 gctggcgagc gcaatcggcg tgcggatggc ttcgagcacg cgctcgagca cgcgctcgcc
    10081 ttcgatgtcc tcgagcagca gcacgaactc gtcgccgccg aggcgggcca cggtgtcgct
    10141 ggcgcgcagg atcgcgccga gccggtcgac gatgccgatc agcacctggt cgccggcttc
    10201 gtggccgagc tgatcgttca ccggcttgaa gccgtcgagg tcgatcacgc agaccgcgag
    10261 cggcttccct gtgcgccgag agtgtgcgat cgcctggcgc aggcggtcgt cgagcaggcg
    10321 gcggttgggt gcgccggtga gcgggtcgaa gtaggcgatg cggttgagct cctcctcatg
    10381 cttcttgagc cggctgatgt cggagaacat cgccagatgg tggatcaccc gaccgcgtgc
    10441 gtcgcgcacg cggctgatcg agagcacctc ggcgaactcg ctgccgtcct tgcgccggtt
    10501 ccacagttcg ccctgccagt ggtcgtgctc ttcgatcgcc tgccacatcg cacggtagaa
    10561 ctcgcgcccc tggcggcccg aggcgagcat gccggggtcg cggccgaggc tctcctcgcg
    10621 gctgtagccg gtgatgcggc agaaggccgg gttgacgtcg acgatgcggt tgtcgacgtc
    10681 ggtgatcagg atcgcctcgt agctcgacga gaacacgctc gcggccaggc gcaggcgctc
    10741 ctcgttctcc ttgcgccgat cgatgtcgat ctgcgcgccg gtcagcagca gcgcgcgccc
    10801 gtccgcgtcg cggcgcacgg cgagcgcgcg gtcatacacc cagatccagc gcccgtcgcg
    10861 atggcgcagg cgcaggtcgt gctcgtagaa ggcggtctcg ccgcggatgt ggcgatcgag
    10921 ctcgcgctcg gcctcgggga ggtcggcggg atgcacgcgc ttgcgccagc acgcgcgccc
    10981 ttcgccgtcg cagtccgccg ccgccagccc gagcatcgcc gcccagcgcg cgtccatctt
    11041 ccactcgccg gtctccagat tccattccca gctgccggcc tgggtggcct cgatcacgtt
    11101 gcgcaggtgg tggcgatcga gttcgatctc tgctgccagg cggcgcgcgg cggcgttggc
    11161 ctgcgacagc gccaggttgc gctgcgccag acgcaccagc agcagcacga tcgccagggt
    11221 cgcgaggccg agtacgaaga tcgtctcgtg gtagcgcgcc cacacgtcgt tccaggtaaa
    11281 gggtggagtg gcgtcgaagg gcggcaggcg cagttcgcgc agcatctcct cgacgcgggc
    11341 gtaatcggcg gggatggtga agccgtggat gcctgcggcg cgcgcggccg gggcggcggt
    11401 ttcgaggccg agcgcgagtg ccgccacctt gcgcatcagg gccgggtcgg cctgacgaag
    11461 ggcgacgagg ggccattcgg ggtagaggcg tgtggaactg cgcagcggga agcccggatg
    11521 ctgcagggga tgcagcacac gcaacccgga gaggtcgcgc ccctcggcga cgagcgcctc
    11581 gaagaggccg gtgcggacga agccggcgtc gacgcggccg tcgagtaccg cttcgacgac
    11641 gctgtcctgg gtctgctgca gcgtgaacat gcgcagtgag ccggggtcga tgccggcggc
    11701 cttcatctcg agtgcctgcg ccggataggt ggcgaggaag ttggcatggg tggtgccgac
    11761 gcgcctgccc cgcaggtcgg cgagggtctt caggctggcg tccccggcgc gcaccaggat
    11821 cacgccgccg aactcgctca gggcctggcc gccggcatcg gcgaccacgg tggcgatggc
    11881 accggagagc ggattggatg cgcgcagctc gatgaaatgc accgggttgg tcagcagcag
    11941 atcgatctcg ccggcggcga gggcgcgctg caggccggcg tcgtcgagcg cgtgcaggcg
    12001 tacctcgacg ccgtacaggt gctggttcag gtagtccacg aagggctgcc acgaggcctc
    12061 gacgagcgcc ttcgggcgcg ccgaatagac gccgaaatcg agctccgtcc gggcgtgcag
    12121 agccgtgctg ccgaggctca ggcagaccag caggacgacg gtgcggagga gtcgtcgtat
    12181 cagcacggga tggccaccga gtccgtcgcg cgatgcgcca gcaggctcga cgtgcgcgca
    12241 cggcggcggc gcgcgggcga gtcttcgggc tggcggagag cgtcacgaca catcgacggg
    12301 agagctatgg gacaactacc cattctaggt ctttcgggat gcctgcggca ggcgattcgg
    12361 cgctgccgtg acgcagttcg cgcagcagcc gggctgcgtg ccccggggca ggggcacgcg
    12421 cccgcgctcc ggccttcagt cctcgcggcg gcggaagggc ttgccggcac gctcgccctg
    12481 gcgcttcacc ggcgcggagt ggccgccagc cttgtggtcg gtggccggcg cgcggcgttc
    12541 gtc
//
LOCUS       DAOQNN010000045        12343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2260_length_12343_cov_9.615641, whole genome shotgun sequence.
ACCESSION   DAOQNN010000045 DAOQNN010000000
VERSION     DAOQNN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2260_length_12343_cov_9.615641"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..681
                     /locus_tag="PKV97_04610"
     CDS             52..681
                     /locus_tag="PKV97_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HNB05191.1"
                     /translation="MFRSVKSASSAVLHFLVLAVAAGLLGGCASSFPPAPASAADADY
                     NYVIGPGDSVNIVVWRNPELSTSVPVRPDGKITAPLVEDLPAMGKDSSTLARDIEKQL
                     AKFIRDPVVTVIVGGFVGPYSEQIRVIGEAANPQTLAYKQKMTLLDVMIAVGGLTDFA
                     DGNGASILRTAEGNKQYSVRIRDLVKRGDVSANVEMRPGDVLIIPQSWF"
     gene            752..2296
                     /locus_tag="PKV97_04615"
     CDS             752..2296
                     /locus_tag="PKV97_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNB05192.1"
                     /translation="MEELVTQIIGYLRGMWRFRWWGLALAWVAGIAGSLAIYFMPDHY
                     ESSARIHVDTQSVLRPLMSGLAVQPNISQQIDLLSRTLISRPNVEKLITMADLDLTVQ
                     NAQQREALITSVTSRLRIQSGRGDNLFTLSYEDVEPHRAQRVVQSLLSLFVESGLGGK
                     RQDTDAARRFIEDQIRSYEQKLTDAENRLKDFRLRNMALLGTGARDYVTQIAETNEQL
                     REARLELREAENSRDALRQQLSAAPSDAGVAPPPMVATPEIDARIDLLKRNLDEMLQR
                     YTEMHPDVVGARRVIEDLEKQKKAQLAELSASMAGSTFGAPGAMSAGVEQTRLAVAQA
                     EARVASLRARVAEFESRLASLSEDAKRIPELETEMAQLNRDYSVHKSNYDQLVSRRES
                     ANIAAEMSTQSGIADFRIIDPPTLPTKPSAPNRLLLLPLAALVGLGAGFALTFLISQL
                     RPAFSDARQLREITGLPVLGTVSMLTTSERRRRRRNGLFAFGSGLVAYAGVFAAVTVA
                     IVFLQS"
     gene            2310..3254
                     /locus_tag="PKV97_04620"
     CDS             2310..3254
                     /locus_tag="PKV97_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HNB05193.1"
                     /translation="MSIIEKAVKRLDQLKSAAGEGAVESAPVPEALVLPEDKPLAPKV
                     ESNAPVVATPAPVRAQPKSVHIDLARLAARGLVTPDQPRSAIAEEFRLIKRPLLANAV
                     LEGPAAVDNGNLIMISSALPGEGKTFNAINLAISMAMELDYTVLLVDADVSRPSVLRE
                     FDLPPQPGLMDVLAGEVEDLSEVLLKTNIDKLSILPAGMPHQRATELLASDGMTQLLE
                     QMAHRYADRIIVFDSPPLLVTTESRVLATHMGQVVLVVEAERTTHAHVRQALAAIEPC
                     PIRMMVLNKSRDRSVGGYYGYGYGYGYGKAPEASSEAA"
     gene            3289..4734
                     /locus_tag="PKV97_04625"
     CDS             3289..4734
                     /locus_tag="PKV97_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HNB05194.1"
                     /translation="MLALLCPATGAVAQSTRVTPTFDTELTWTDNAGLDREGGRDWLL
                     EVSPGVSVSRSAGRLNGSLSARLRNLVHSSASEDDSTYLSFDGRGAYEALEDLLFLDF
                     SGGITRNNYSAFSGRAPGDPFGFDKDNEVRYWSITPRLESDLRFGEAARGSVSYSSSG
                     VRSNSSGLGDQSADTVEASLADPGGLRFFGWGLAYARTESDGGTAATGQRGEESVRGT
                     LYANVSPQLRLRGIVGHESNDYADGRKDSSGIVGAGVDWYPTDRTSLAATVEDRVFGR
                     GYDLALNHRMRRAVFFLGATRDVSSLAQTLGTVVLADRDCFMRVRDIADPIEREQALN
                     ECLTFGVLRSNAAYVERAFRGGVSLLGVRNTLSLSASRSDRSRIGSTTGLLPEDDLRD
                     TERVRITTTTLAWSHTLSGTSSLYATLARSDSESAADADLALRRLTGTVGVDRTLGPR
                     TRAGLRYRYEKTEGESDYTTNSVTAILGMRF"
     gene            4746..5840
                     /locus_tag="PKV97_04630"
     CDS             4746..5840
                     /locus_tag="PKV97_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated ATPase"
                     /protein_id="HNB05195.1"
                     /translation="MYETFFKLSGKPFQLNPDPAFFYGSRGHRRAMAYLEYGLHQSEG
                     FIVITGEVGAGKTTIVRSLLEQLDPAKVVAATIVSTQIDASDMLRMVAGAFGVPSRNL
                     DKAGLLLAIETYLVSLAAADKRALLIIDEAQNLSAAAVEELRMLSNFQLEDHALLQSF
                     LVGQPEFREMMQRAEMQQLRQRVIASYHLGPLDLQETRGYIEHRLRHVGWNEDPRFEP
                     GSFNAIYGHTGGIPRTINTLCDRLLLAAYLGESHVVTEAQVREVIAELKEEFAPPRTR
                     PVAGMGRAVLRDAEPAAAMPPVEALAVERLRVSPELADQVAGMAGSYDLQRIEARLAG
                     LEQSVSATLAVLNQLLQAVRRDAATEEEGR"
     gene            5837..7003
                     /gene="wecB"
                     /locus_tag="PKV97_04635"
     CDS             5837..7003
                     /gene="wecB"
                     /locus_tag="PKV97_04635"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNB05196.1"
                     /translation="MSAHRLSAAPVICIVGARPNYMKVAPIIRAFAAHQPPIPTLLVH
                     TGQHYDPAMKDRLFADLDLPEPDVNLAVGSGSHAVQTAEVMKRFEPVIDEHGARAVLV
                     VGDVNSTLACALVASKRHIPVVHVESGLRSNDRRMPEEINRILTDQISDVLYTTERSA
                     HDNLVREGIPAERAVFVGNVMIDSLRASLPRAVPADVLLAAAGLDASRIAGGYGVVTL
                     HRPSNVDSADTLGPIIDALRTVSEALPLVVALHPRTRNNLERFGMLDRLAGSGFLILP
                     PQGYLEMLGLMSGARMVLTDSGGIQEETTALGVPCVTIRENTERPITVEQGTNTLVGI
                     RPEAIVAAAREILATGGKAGRVPEYWDGRCAERIAAHLHDWLVEHETVHAAPGG"
     gene            7005..7844
                     /locus_tag="PKV97_04640"
     CDS             7005..7844
                     /locus_tag="PKV97_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HNB05197.1"
                     /translation="MAARITNAFTCDVEDYFQVSALAPHFPREQWDSVPCRVERNVDR
                     ILALLDGHGARGTFFTLGWLAERYPGLVRRIAAAGHEVASHGYAHQRASDLTPEAFTA
                     DIRLAKVILEDITGSAVTGYRAPSFSIGEANLWAHDCIAEAGYRYSSSVYPVRHDHYG
                     IPDAPRFPWRLPNGLVEVPITTVHMLGRNWPAGGGGFFRLLPYALSRWQIARVNAADR
                     RPAIFYFHPWEIDPEQPRVTAATAKTRFRHYINLQHTEARLDRLLSDFSWGRADEVFR
                     DAA"
     gene            7870..8919
                     /locus_tag="PKV97_04645"
     CDS             7870..8919
                     /locus_tag="PKV97_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HNB05198.1"
                     /translation="MDRLPIVVNLLKAEDALRWDEFVRRCPQATFFHLSAWRELMEEV
                     FDHRTFYLYAERAGDIVGVLPLAEVRSRLFGHALVSLPFCVYGGIAAAEDAGPETLYA
                     LESKAETLAQALGVQHLEFRNLKPRHEDWPRQELYVTFRKEILPEVEANLLAIPRKQR
                     AMVRKGIKNGLVSEVDDHVDRFFALYADNVLRHGTPALPRKFFEQLKERFGEACEVLT
                     VTSPEGKPLSSVLSFYFRDEVLPYYAGDDLAARELAANDFKYWELMRRACERGCTVFD
                     YGRSKVGTGPYAFKKNWGFEPQPLSYEFCLYKRDSIPQNNPMNPKYRAFIALWKRLPI
                     GVANRLGPHIVRNLG"
     gene            8924..10213
                     /locus_tag="PKV97_04650"
     CDS             8924..10213
                     /locus_tag="PKV97_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNB05199.1"
                     /translation="MQDSRPPLLYLTHRIPYPPNKGDKLRSFNILRQLARTHRVWLGS
                     FVDHADDRRHIPALRQWCEQTCVIPIEPGIRRFASLRGLLRGEALSLPYYRSPRLARW
                     VDQVVAENGIRSAVAFSGPMAQYLDVPGLSRRVVDFCDVDSAKWTQYAADRRWPMSWL
                     YRREGERLLDFERRAAAGVEASLFVTEAEAEVFRRAAPEVGDRVGVMQNGVDAEYFAP
                     ANAGASPYPAGAPVIAFSGAMDYWPNVDAVCWFATELLPAIRRAMPGVRFWIVGMNPA
                     PAVQALAGEDVVVTGTVPDVRPYIAHADLVVAPLRIARGIQNKVLEAMAMARPVVLSA
                     APAVGLAARDGVECSIADDGDELCRRVVELLGDPSRRAAIGAAARDCVLRAYSWSAHL
                     RTLDWLLDAQGTDAGLAQAVQAREGAAPNAPALVRTP"
     gene            10210..11376
                     /locus_tag="PKV97_04655"
     CDS             10210..11376
                     /locus_tag="PKV97_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNB05200.1"
                     /translation="MKDVARPLVLHLVYRFDVGGLENGVVNLINHLPAERFRHAVVAL
                     TECTEGFSRRIERADVRFVSLHKPPGHGLKLYPRLYRLFRALRPAIVHTRNLAALEAV
                     VPARLAGVPATVHGEHGWDVSDPGGTRRKFQWLRRAYSPFVNRYVALSGHIESYLVER
                     VGISPARVARICNGVDARRFHPRLSAQARSLAGLPEGFATPGTVIVGTVGRQQAVKDP
                     LALVRAFALARTHGEVGGRLRLLLAGDGPLRATLEAEIRSSGLEDAVWLAGERADVPA
                     VMRALDVFALPSRAEGISNTVLEAMASGLPVVATAVGGNAELVVPGETGALVAAEDPR
                     GMAEALLCYAADAAVRQEQGLAARRRVEAEFSIERMVGRYAELYESLLRPQGAA"
     gene            11392..>12343
                     /gene="asnB"
                     /locus_tag="PKV97_04660"
     CDS             11392..>12343
                     /gene="asnB"
                     /locus_tag="PKV97_04660"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNB05201.1"
                     /translation="MCGIAGLFDTRGKRDFERALMQRMNDVQHHRGPDEGGYHFEPGV
                     ALAHRRLSIIDLATGQQPLFNEDGSVAVVFNGEIYNFQELVPELEALGHVFHTRSDTE
                     VIVHAWEAWGEDCVQRFRGMFAFALHDRKRDTFFMARDRLAVKPMFYALLPDGTLAFG
                     SELKVLMQHPALDRRIDPLAVEEYFALGYVAEPRTIFLGARKLGPGESLCIRRGQPIP
                     APKQYWDVRFTLDNDIGLADATAELTERLRESVRLRMIAEVPLGAFLSGGVDSSAVVA
                     TMAGLSDDPVNTCSIAFDDPKYNESAFAQMVADRYRTRHFV"
BASE COUNT         1883 a         4442 c         4103 g         1915 t
ORIGIN      
        1 ttcgcgtcgt cgtatatcat tcgccgtccg ggacttacga aggggcttgt catgttcaga
       61 agcgtcaaaa gcgcatcatc cgcggtgttg catttcctcg ttctcgctgt ggcggcggga
      121 ctgctcggtg gttgtgcctc ttccttcccg cctgcccccg cgtccgcggc cgatgcggac
      181 tacaactacg tgatcgggcc cggggatagc gtcaatatcg tggtctggcg caaccccgag
      241 ctgtccacct ccgtcccggt ccggccggac gggaagatca cggcaccgct ggtcgaagac
      301 ctgcccgcga tgggcaagga ttcgagcacg ctggcccgcg acatcgagaa gcagcttgcg
      361 aagttcatcc gcgatccggt cgtgacggtg atcgtcggtg gcttcgtcgg tccttacagc
      421 gaacagatcc gcgtgatcgg cgaggcggcc aatccgcaga cgctggcgta caagcagaag
      481 atgaccctgc tcgacgtgat gatcgcagtc ggcgggctca cggacttcgc cgacggtaac
      541 ggcgcgagca tcctgcgcac cgccgagggc aacaagcaat acagcgtgcg catccgtgac
      601 ctcgtcaagc gcggcgacgt ctcggcgaac gtcgagatgc gccctggcga cgtgttgatc
      661 atcccgcaga gctggttctg acctccatcc atacacgccc gcaagccgat cgcagaaggt
      721 cggtggcggg cgctcagcac gaccgtgacc catggaagaa ctcgtaacac agatcatcgg
      781 ctacctgcgc ggcatgtggc ggttccgctg gtgggggctt gcgctcgcgt gggtcgcagg
      841 tatcgccggt agcctggcga tctacttcat gccggaccac tacgagtcgt ccgcgcgcat
      901 ccacgtggat acccagtcgg tgttgcgccc gctgatgtcc gggctcgccg tccagcccaa
      961 catcagccag cagatcgact tgctcagccg cacgctgatc agccggccca acgtcgagaa
     1021 gctgatcacc atggccgacc tcgacctcac ggtgcagaac gcacagcagc gcgaggccct
     1081 gatcacctcg gtgaccagcc ggctgcgcat ccagtccggc cgcggcgaca acctgttcac
     1141 gctctcgtac gaagacgtcg agccgcatcg tgcgcagcgc gtcgtgcagt cgctgctgtc
     1201 cctgttcgtc gaatccggtc tcggcggcaa gcgtcaggat accgacgcgg cacggcgctt
     1261 catcgaggac cagatccgca gctacgagca gaagctgacc gacgccgaga accggctcaa
     1321 ggacttccgc ctgcgcaaca tggcgctgct gggtaccggc gcgcgcgatt acgtcaccca
     1381 gatcgccgag accaacgagc aattgcgcga agcacgcctg gaactgcgcg aggccgagaa
     1441 ctcgcgcgac gcgctccgcc agcaactctc cgcagcgccg tccgatgcgg gcgtcgcgcc
     1501 gccgccgatg gtggcgacgc ccgagatcga tgcccgcatc gacctgctca agagaaacct
     1561 cgacgagatg ttgcagcgct acaccgagat gcacccggac gtggtcggtg cacgccgcgt
     1621 catcgaggat ctcgagaagc agaagaaggc gcagctggcc gagctgagcg cgagcatggc
     1681 gggcagtacc ttcggtgctc ccggtgccat gagtgcgggg gtcgagcaga cccggctcgc
     1741 ggtggcgcag gccgaggccc gggtcgcgtc gctgcgtgcg cgggtggccg agttcgagag
     1801 ccgcctggcg agcctctcgg aggacgccaa gcggatcccc gagctcgaga ccgagatggc
     1861 gcagctcaac cgcgattaca gcgtccacaa gagcaactac gaccagctgg tatcgcgtcg
     1921 ggagtcggcg aacatcgccg ccgagatgag cacccagtcg ggcatcgccg acttccgcat
     1981 catcgatccg cccaccctgc ccaccaagcc ctcggctccc aaccggctgc tgctgctgcc
     2041 gctcgccgcg ctggtgggtc tgggggcggg gttcgcgctc accttcctga tcagccagct
     2101 gcgaccggcg ttcagcgatg cacgccagct gcgcgagatc accggcctgc cggtgctcgg
     2161 caccgtctcc atgctgacca cttcggagcg tcgtcgtcgt cgccgcaacg ggctgttcgc
     2221 cttcggcagc ggtctggtgg cgtacgccgg ggtgttcgcc gcggttacgg tcgcgatcgt
     2281 gttcctgcag agttgaacgg ggtgcccgga tgagcattat cgaaaaagca gtcaagcgcc
     2341 tcgaccaact caagagtgcg gcgggcgagg gcgccgtcga atccgctccg gtgccggagg
     2401 cgctcgtcct gcccgaggac aagccgcttg cgcccaaggt ggagtcgaat gcgccggtgg
     2461 tcgcgacccc cgcgccggtg cgcgcccagc ccaagagcgt ccatatcgat ctcgcgcgcc
     2521 tggccgctcg cggcttggtc acgcccgacc agccgcgctc ggccattgcc gaggagttcc
     2581 gcctgatcaa gcgtcccttg ctggccaatg ccgtcctcga ggggccggcg gcggtcgata
     2641 acggcaacct gatcatgatt tccagcgcgc ttcctggcga gggcaagacc ttcaacgcga
     2701 tcaatctcgc gatcagcatg gcgatggagc tcgactacac ggtgctgctg gtggacgccg
     2761 acgtgtcgcg tccctcggtg ctgcgcgagt tcgatctgcc gccgcagccg ggactgatgg
     2821 acgtgctcgc cggcgaggtc gaggacctct ccgaggtcct gctcaagacc aacatcgaca
     2881 agctgtccat cctgccggcg ggcatgccgc accagcgcgc gaccgaactg ctcgcctcgg
     2941 acggcatgac gcagttgctc gagcagatgg cgcatcgtta cgcggaccgg atcatcgtgt
     3001 tcgactcgcc gccgctgctg gtcacgaccg aatcgcgcgt gcttgccacc cacatgggac
     3061 aggtggtcct cgtggtggag gccgagcgca ccacccacgc ccacgttcgc caggcgctcg
     3121 cagcgatcga gccctgcccg atccgcatga tggtgctcaa caagtcgcgt gatcgctcgg
     3181 tggggggcta ctacggctat ggatacggat acggatatgg gaaagcgccc gaagccagtt
     3241 ccgaggcggc ctgagccccg gcgtgccggc ctcgtcgccg gtgcgtgcgt gctcgcgctc
     3301 ctgtgcccgg ccaccggagc ggtcgcgcag tcgacccggg tcacccccac gttcgacacc
     3361 gaactcacct ggaccgacaa cgccggcctc gatcgcgagg gcggtcggga ctggttgctc
     3421 gaggtctcgc ccggcgtgtc ggtgagccgc agcgcagggc gtctcaacgg cagcctgagc
     3481 gcgcgcctgc gcaacctcgt ccattcctct gcgagcgagg acgactccac ttacctctcc
     3541 ttcgacggca ggggcgccta cgaggcgctg gaggatctgc tgttcctcga tttcagcggg
     3601 ggaatcaccc gcaacaacta ttccgccttc agcgggcggg cgcccggcga cccgttcggt
     3661 ttcgacaagg acaacgaagt ccgctattgg tcgatcaccc cgcggctcga gtccgacctg
     3721 cgcttcggcg aggctgcacg cggttcggtg agctattcgt ccagcggcgt gcgatccaac
     3781 agcagcggtc tcggcgacca gagcgcggac accgtggagg ccagcctcgc cgatccgggc
     3841 ggcttgcggt ttttcggctg gggcctggcc tatgcccgca ccgaaagtga tggcggcacc
     3901 gccgccaccg gccagcgtgg cgaggagtcg gtgcgtggca cgctgtacgc caacgtctcc
     3961 ccgcagctgc gcctgcgcgg catcgtcggg cacgagtcca acgactacgc ggacggcagg
     4021 aaggacagca gcgggatcgt tggcgccggg gtggactggt acccgaccga ccgcacctcg
     4081 ctcgccgcga cggtcgagga tcgggtgttc gggcgcggct acgatctcgc cctcaaccac
     4141 cgcatgcgcc gcgcggtctt cttcctcggt gcgacgcgcg acgtctcctc gctcgcacag
     4201 acgctgggca cggtggtgct cgcggatcgg gactgcttca tgcgcgtacg cgacatcgcc
     4261 gatccgatcg agcgcgagca ggccctcaac gaatgtctca ccttcggcgt gctgcgctcc
     4321 aatgccgcct acgtcgagcg cgcgttccgt ggcggcgtct cgctgctcgg ggtgcgcaac
     4381 accttgagct tgtccgccag tcgcagcgat cgcagccgca tcggttcgac caccggcctg
     4441 cttccggaag acgacctgcg cgacaccgag cgggtgcgca tcaccaccac cacgctcgcc
     4501 tggagccaca cgctcagcgg cacgagttcg ctgtatgcca cgctcgcgcg ctcggattcc
     4561 gagagtgcgg ccgacgccga cctcgcactg cgtcgcctga ccggcacggt gggcgtcgat
     4621 cgtacgctcg gccccaggac gcgcgcgggg ctgcgctacc gatatgaaaa gaccgagggc
     4681 gagagcgact acacgacgaa ttccgtcacc gccatcctcg gcatgcggtt ctgaacggac
     4741 acccgatgta cgaaaccttc ttcaaactca gcggcaagcc tttccagctc aaccccgacc
     4801 cggccttctt ctacggcagt cgggggcacc ggcgcgcgat ggcctatctc gaatacggcc
     4861 tgcaccagag cgagggcttc atcgtcatca ccggcgaggt gggcgcgggc aagaccacca
     4921 tcgtgcgcag cctgctcgag cagctcgatc cggccaaggt cgtcgccgcc accatcgtca
     4981 gcacccagat cgacgcctcc gacatgctgc gcatggtcgc cggcgccttc ggcgtaccca
     5041 gccgcaacct cgacaaggcc ggcctgctgc tcgcgatcga gacctacctc gtgtcgctcg
     5101 ccgccgccga caagcgcgcg ctgctgatca tcgacgaggc gcagaacctc tccgcagccg
     5161 cggtcgagga actgcgcatg ctgtcgaact tccagctcga ggatcatgcg ctgctgcaga
     5221 gtttcctcgt cggccagccc gagttccgcg agatgatgca gcgcgccgag atgcagcaac
     5281 tgcgccagcg cgtgatcgcc tcctatcacc tcggcccgct cgacctgcag gagacgcgcg
     5341 gctacatcga gcaccgcctg cgccacgtcg gctggaacga ggacccgcgc ttcgagcccg
     5401 ggtccttcaa cgcgatctac ggccacaccg gcggcatccc ccgcaccatc aacaccttgt
     5461 gcgaccgtct gctcctcgcc gcctatctcg gcgagagcca tgtggtcacc gaggcgcagg
     5521 tgcgcgaggt gatcgccgag ctcaaggaag agttcgcgcc gccgcgtacg cgcccggtgg
     5581 ccggcatggg acgcgcggtg ctgagggacg ccgagcccgc tgcggcgatg ccgccggtcg
     5641 aggcgctggc agtcgagcgc ctgcgcgtct cgcccgagct tgccgaccag gtcgccggca
     5701 tggccggcag ctacgacctg cagcgcatcg aggcacgtct ggccggcctg gagcagtcgg
     5761 tgtcggccac gctcgcagtg ctcaaccagt tgctgcaggc ggtgcgccgc gatgccgcga
     5821 ccgaggagga aggtcgatga gcgcgcatcg cctctcggcc gcgccggtga tctgcatcgt
     5881 cggcgcacgt cccaactaca tgaaggtggc gccgatcatc cgcgccttcg ccgcccacca
     5941 gccgccgatc cccacgctgc tggtgcacac cgggcagcat tacgatccgg cgatgaagga
     6001 ccgcctgttc gccgatctcg acctgcccga gcccgacgtc aacctcgcgg tcggctccgg
     6061 ctcgcacgcg gtgcagaccg ccgaggtgat gaagcgcttc gagccggtca tcgacgagca
     6121 tggcgcgcgc gcggtgctgg tggtgggcga tgtcaactcg acgctcgcct gcgcgctggt
     6181 ggcgagcaag cgccacatcc cggtggtcca tgtggagtcc ggtctgcgca gcaacgaccg
     6241 ccgcatgccc gaagagatca accgcatcct caccgaccag atctccgacg tgctctacac
     6301 caccgagcga tccgcccacg acaacctcgt ccgcgaaggc atccccgccg agcgtgcggt
     6361 tttcgtcggc aacgtcatga tcgacagcct gcgcgcgagc ctgccgcgcg cggtgccggc
     6421 cgacgtgctg ctcgccgcgg ccggcctgga cgcgagccgg atcgccggcg gctacggcgt
     6481 cgtcaccctg caccgtccgt ccaacgtcga cagcgccgac accctcggcc cgatcatcga
     6541 cgccctgcgc acggtgagcg aggcgttgcc gctggttgtc gcgctgcatc cgcgcacacg
     6601 caacaacctc gagcgcttcg gcatgctcga tcgcctcgcc ggctccggct tcctgatcct
     6661 gccgccgcag ggctacctcg agatgctcgg cctgatgtcg ggtgcgcgca tggtgctgac
     6721 cgactccggc ggcatccagg aagagaccac cgcgctcggc gtgccctgcg tgaccatccg
     6781 cgagaacacc gagcgcccga tcacggtgga gcagggcacc aacaccctgg tcggcatccg
     6841 ccccgaggcg atcgtcgccg cggcgcgcga gatcctcgcc accggtggca aggccgggcg
     6901 ggtgcccgaa tactgggatg gacgctgcgc cgagcgcatc gctgcccatc tgcacgactg
     6961 gctcgtcgag cacgagacgg tccacgccgc gccggggggt tgaggtggcc gcacgcatca
     7021 ccaacgcctt cacctgcgac gtcgaggact acttccaggt ctccgcgctc gcgccgcatt
     7081 ttccgcgcga gcagtgggac agcgtgccct gccgcgtcga gcgcaacgtc gatcgcatcc
     7141 tcgcgctgct cgacggccac ggcgcgcgcg gcaccttctt caccctgggc tggctcgccg
     7201 agcgctatcc cggcctggtg cgccgcatcg ccgccgcggg ccacgaggtg gcgagccacg
     7261 gctacgcgca ccagcgcgcg agcgatctga cgcccgaggc cttcacggcc gacatccgcc
     7321 tggccaaggt gatcctcgag gacatcaccg gtagtgcggt gaccggctac cgcgcgccca
     7381 gcttctcgat cggcgaggcc aacctgtggg cgcacgactg catcgccgag gcgggctacc
     7441 gctacagctc gagcgtgtat ccggtccgcc acgaccacta cggcatcccc gacgcgccgc
     7501 gcttcccgtg gcgcctgccc aacggcctgg tcgaggtgcc gatcaccacc gtgcacatgc
     7561 ttggacgcaa ctggcccgcg ggtggcggcg gtttcttccg cctgttgccc tatgcgctgt
     7621 cgcgctggca gatcgcgcgc gtcaatgccg ccgaccgccg cccggcgatc ttctacttcc
     7681 acccctggga aatcgatccc gagcagccgc gtgtgaccgc tgccacggca aagacccgct
     7741 tcaggcacta cattaacctg cagcacacgg aagcccggct cgaccggctt ctctccgatt
     7801 tctcctgggg gcgcgccgac gaagtcttcc gcgacgccgc ctgaacccga ctggatccgg
     7861 atgcgcgcca tggaccgatt gccgatcgtc gtgaacctgc tcaaggccga ggacgccctg
     7921 cgctgggacg aattcgtgcg ccgctgcccg caggccacct tcttccacct gtccgcctgg
     7981 cgcgagctca tggaagaggt gttcgatcac cgcaccttct atctgtatgc cgagcgcgcc
     8041 ggcgacatcg tcggcgtgct gccgctcgcc gaggtgcgca gccgcctgtt cggtcatgcg
     8101 ctggtgtcgc tacccttctg cgtctacggc ggcatcgcgg cggccgagga tgcaggcccg
     8161 gagaccctgt acgcgctcga atccaaggcc gagacgctgg cgcaggcgct cggcgtgcag
     8221 cacctcgaat tccgcaacct gaagccgcgc cacgaggact ggccgcgcca ggaactctac
     8281 gtcaccttcc gcaaggagat cctgcccgag gtcgaggcca acctgctcgc catcccgcgc
     8341 aagcagcgcg ccatggtgcg caagggcatc aagaacgggc tggtgagcga ggtggacgac
     8401 catgtcgacc gcttcttcgc gctctacgcc gacaacgtgc ttcgccacgg cacgccggcg
     8461 ctgccgagga agttcttcga gcagctcaag gagcgcttcg gtgaggcctg cgaagtgctc
     8521 acggtgacct cgcccgaggg caagccgctg tcgagcgtgc tcagcttcta cttccgcgac
     8581 gaggtgctgc cctactacgc gggcgacgac cttgcagcgc gcgagctcgc cgccaacgac
     8641 ttcaagtact gggagctgat gcggcgcgcc tgcgagcgcg gctgcacggt cttcgactac
     8701 ggtcgcagca aggtcggcac cggcccctat gcgttcaaga agaactgggg cttcgagccg
     8761 cagccgctgt cgtacgagtt ctgcctgtac aagcgcgaca gcatcccgca gaacaacccg
     8821 atgaacccca agtaccgggc cttcatcgcc ctctggaagc gcctgccgat cggcgtggcg
     8881 aaccggctcg ggccgcacat cgtgcgcaac ctggggtagg gcggtgcagg acagccggcc
     8941 gccgctcctc tacctcacgc accgcatccc ctacccgccc aacaagggcg acaagctgcg
     9001 gtcgttcaac atcctgcgcc agctcgcccg cacgcaccgg gtgtggctgg gcagcttcgt
     9061 cgaccatgcg gacgaccgcc gccacatccc ggcgctgcgc cagtggtgcg agcagacctg
     9121 cgtgatcccg atcgagccgg gcatccgccg cttcgcgagc ctgcgcggcc tgctgcgcgg
     9181 cgaggcgctg agcctgccct attaccgcag cccgcgcctg gcgcgctggg tggatcaggt
     9241 ggtggcggag aacggcatcc gttcggcggt ggccttctcg gggccgatgg cgcagtacct
     9301 cgacgtgccc gggctgagcc gccgcgtggt cgacttctgc gacgtcgatt cggccaagtg
     9361 gacgcagtac gccgccgacc ggcgctggcc gatgtcctgg ctgtaccggc gcgagggcga
     9421 gcgcctgctc gacttcgagc gccgcgccgc agccggcgtg gaggcgagcc tcttcgtcac
     9481 cgaggccgag gccgaggtct tccggcgcgc ggcgcccgaa gtcggcgacc gcgtcggcgt
     9541 catgcagaac ggggtcgacg ccgagtactt cgcgcctgcc aacgcggggg cttcgcccta
     9601 tccggcgggc gctccggtga tcgccttcag cggtgcgatg gactactggc ccaacgtcga
     9661 cgcggtgtgc tggttcgcca ccgagctcct gccggcgatc cgtcgcgcca tgcccggcgt
     9721 gcgcttctgg atcgtgggca tgaacccggc gccggccgtg caggcgctcg ccggcgagga
     9781 tgtcgtggtc accggtaccg tgcccgacgt gcgcccctac atcgcccacg ccgacctcgt
     9841 ggtggcgccc ctgcgcattg cgcgcggcat ccagaacaag gtgctggagg cgatggcgat
     9901 ggcgcgcccg gtggtgctct cggccgcgcc cgccgtcggt ctggcggcgc gcgacggggt
     9961 ggagtgcagc atcgccgacg atggcgacga gctgtgccgg cgcgtggtcg agctgcttgg
    10021 ggatccgtcg cgtcgcgccg cgatcggcgc ggcggcgcgg gactgcgtgc tgcgcgccta
    10081 cagctggtcg gcgcacctgc gcaccctgga ctggctgctc gatgcgcagg gcacggacgc
    10141 aggcctcgcg caggcggtgc aggcccgcga gggcgccgcg ccgaacgcgc cggcgctcgt
    10201 gcggacacca tgaaggacgt cgcccggccg ctggtgctgc acctcgtgta ccgcttcgac
    10261 gtcggcggcc tggagaacgg ggtggtgaac ctgatcaacc acctccctgc cgagcgcttc
    10321 cgtcatgcgg tggtggcgct caccgaatgc accgaaggct tctcgcgccg catcgagcgc
    10381 gcggacgtgc gcttcgtgtc gctgcacaag ccgcccggcc acggcctcaa gctctatccg
    10441 cgcctgtacc ggctgttccg cgcgctgcgt ccggcgatcg tgcatacgcg caacctcgcc
    10501 gcgctcgagg ccgtcgtgcc ggcgcggctc gccggcgtgc cggcgacggt gcatggcgag
    10561 cacggctggg acgtctccga ccccggcggc acgcggcgca agttccagtg gctgcggcgc
    10621 gcctacagcc ccttcgtgaa ccgctacgtg gcgctctcgg gccacatcga gtcctatctc
    10681 gtcgagcgcg tgggcatttc ccccgcgcgg gtcgcgcgca tctgcaacgg cgtggacgcg
    10741 cggcgcttcc acccccgcct gtcggcgcag gcgcgcagcc tcgccggtct gccggaaggc
    10801 ttcgccaccc ccggcacggt catcgtcggc acggtcgggc ggcagcaggc ggtcaaggat
    10861 ccgctcgccc tggtgcgcgc cttcgcgctc gcgcgcacac acggcgaggt tgggggcagg
    10921 ctgcgcctgc tgctcgcggg cgacgggccg ctgcgcgcca cgctcgaggc cgagatccgc
    10981 agcagcgggc tggaagacgc cgtctggctg gccggcgagc gcgccgacgt gcccgcggtg
    11041 atgcgcgcgc tcgacgtgtt cgcgctgccg tcgcgcgccg agggcatctc caacaccgtc
    11101 ctcgaggcga tggcgagcgg gctgccggtc gtcgccaccg cggtcggcgg caatgcggaa
    11161 ctggtcgtgc ccggcgagac cggcgcgctc gtggcggccg aagacccgcg cggcatggcg
    11221 gaggcgttgc tgtgctacgc tgcggatgct gcagtgcggc aagaacaggg cctggccgcg
    11281 cgccggcgcg tcgaggcgga gttcagcatc gagcgcatgg tgggccgcta cgccgagctc
    11341 tacgaatccc ttctgcgccc gcagggcgca gcctgacgag gacacgcgat catgtgcggc
    11401 attgccggcc tcttcgacac ccgtggcaaa cgcgacttcg agcgcgccct gatgcagcgc
    11461 atgaacgacg tccagcacca tcgcggcccg gacgagggcg gctaccactt cgagccgggc
    11521 gtcgcgctcg cccaccgccg cctgtccatc atcgacctcg ccaccggcca gcaaccgctg
    11581 ttcaacgagg acggctcggt ggcggtggtg ttcaacggcg agatctacaa cttccaggag
    11641 ctcgtacccg agctcgaggc gctcggtcac gtcttccaca cccgcagcga cacggaggtc
    11701 atcgtgcacg cctgggaggc ctggggcgag gactgcgtgc agcgcttccg cggcatgttc
    11761 gcgttcgcgc tgcacgaccg caagcgcgac accttcttca tggcgcgcga ccgcctcgcg
    11821 gtcaagccga tgttctacgc gctgctgccc gacggcacgc tcgccttcgg ctccgaactc
    11881 aaggtgctga tgcagcaccc ggcgctcgac cgccgcatcg acccgctcgc ggtcgaggag
    11941 tacttcgcgc tcggctacgt cgccgagccg cgcaccatct tcctcggcgc caggaagctc
    12001 ggccccggcg aatcgctgtg catccgccgc ggccagccga tccccgcgcc gaagcagtac
    12061 tgggacgtgc gcttcaccct cgacaacgac atcggcctcg ccgacgccac cgccgagctg
    12121 accgagcgcc tgcgcgaatc ggtgcggctg cgcatgatcg ccgaggtgcc gctgggtgcc
    12181 ttcctctccg gaggggtcga ttcgagcgcg gtggtggcga ccatggcggg gctctcggac
    12241 gatccggtca acacctgctc gatcgccttc gacgatccca agtacaacga gtcggcgttc
    12301 gcgcagatgg tcgccgaccg ctaccgcacc cgccacttcg tcg
//
LOCUS       DAOQNN010000046        12292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2276_length_12292_cov_8.889107, whole genome shotgun sequence.
ACCESSION   DAOQNN010000046 DAOQNN010000000
VERSION     DAOQNN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12292
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2276_length_12292_cov_8.889107"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PKV97_04665"
     CDS             <1..483
                     /locus_tag="PKV97_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNB05202.1"
                     /translation="VLVPLVVRDEVPSVLLTRRTDHLHHHPGEISFPGGRLEDDDPSP
                     VHAALRETEEEIGLARRHVELIGALPDYFTGTGFRVTPVVGVVHPPLELTLDAFEVAE
                     AFEVPLAHFLDPRNHQEHSILHEGRMRRFHAMPWHGYFIWGATAGILMSLYRLLNEEG
                     "
     gene            596..1519
                     /locus_tag="PKV97_04670"
     CDS             596..1519
                     /locus_tag="PKV97_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HNB05203.1"
                     /translation="MTFFSLILALVLEQLRPLPARGWTVRGVRGVVGGMRGRFEGGSA
                     AQGRLAWLLLVPAAGFASALAFWLLWEMHPALAFLFNVVALYLCIGFRHDTRFFSAIH
                     MALRLGEPEGARSLLEDWRGTRHEGASASEIARLAIEEALVVSHRRLFGVMFWFVLLP
                     GPSGAVMYRLAGLLAEEWGRPDGGDAGFGGFALRAREAIEWLPARITAIVFSIMGNFE
                     DAIYCWRSQAVLWPERSSAILIASGGGALGVRLGMPVHVAGGIVDRPELGLGSEAGVD
                     HMQNTVSLLWRALFVCLFLLFLLAAAGWVGR"
     gene            1549..2730
                     /locus_tag="PKV97_04675"
     CDS             1549..2730
                     /locus_tag="PKV97_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HNB05204.1"
                     /translation="MANREVVVLSAVRSAVGGFGGSLKDMEPSDLGAVVIKEAIARAG
                     VDPKAVSFASVGHCIPTDSRYAYVSRVATINAGMSMDSVAFQVSRLCGSAQQAIVSSA
                     QAIMLGDADFAIGGGVEVMSRGTYLLPALRSGARMGDTKAIDSMVAVLTDPFGVGHMG
                     ITAENLCTKHGITREEQDAFALESQKRAAAAIAEGRFKGQIVPITFETRKGTVVFDTD
                     EHPRMTTMEALGKMKPAFKKDGSVTAGNASGINDAAAFLVLADAAKAAAGGHKPIARL
                     VSYAIAGVPNDVMGEGPIPATKLALQRAGLKLDQIDVIESNEAFAAQSITVNRGLGLD
                     TAKVNPNGGAISLGHPVGATGSVIITKLLHELIRTNGRYGVATMCIGGGQGITTVWER
                     V"
     gene            complement(2964..3365)
                     /gene="msrB"
                     /locus_tag="PKV97_04680"
     CDS             complement(2964..3365)
                     /gene="msrB"
                     /locus_tag="PKV97_04680"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNB05205.1"
                     /translation="MAREVDKPDAEWRRQLSDLQYRVTREKGTERAFTGEYWDVWDKG
                     AYNCVCCGAPLFRSEHKFDAGCGWPSFWTAAEPDNVETAVDRSHFMVRTEVLCHQCGA
                     HLGHVFEDGPQPTGLRYCINSASVRLEKESD"
     gene            complement(3383..4552)
                     /locus_tag="PKV97_04685"
     CDS             complement(3383..4552)
                     /locus_tag="PKV97_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HNB05206.1"
                     /translation="MKRTRLKRRGGLGRTAEQLVWLAGGLAQSGSRIEDHYWETQLAR
                     LIDETLTDNDEEVLNAALDHLYSADARAYDELADNIESRAECAAGSLAEHDVVLIAAP
                     VLAWSRYRIPAANIPPAVLANLRVHLQAHVLAKGAKLSVADFLFSPDQLPQGYCATAE
                     FAKVICGAAADNLDLHIDTDGMPETAQFLSDTRYLLAAVAVPRGAALFRWQEPDGTRD
                     GALEQWRAQGGACLTPLLPGCAVDLVLPDSYFAASRAADKDSRPYSVRASVAFLATTL
                     ETPAANLRAVVAPFWERTLEEYRIGFTVRGRDEVVHGVVWPLLGAEDETTDCVAQIET
                     VLRESGLTDVVALEHHFPLEYCDDCGAPLYPSPEGEVVHAEMPEEQGVQAPRHLH"
     gene            complement(4659..5492)
                     /locus_tag="PKV97_04690"
     CDS             complement(4659..5492)
                     /locus_tag="PKV97_04690"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNB05207.1"
                     /translation="MNDIARRTVFFVSDGTGITAETLGHSLLAQFPEARFRQVRAPFI
                     DDIDKAIDCATQIREAAAEDGVRPIVFSTLVNQATVDALHKSDALFLDLFERFIGPLE
                     AELGQRSTHAVGRFHGIADSLNYKYRIEAINFAMAHDDGISSEGELAEADVILVGVSR
                     SGKTPTSLYLAMQFGVKAANYPLIPEDFERNKLPGELHKYRTKLFGLTIAPERLSQIR
                     QERRPNSRYASLENCRYEIDAAHKLMRRENIRCLDSTTKSIEEISATILQTIRVDRPG
                     F"
     gene            5630..7996
                     /gene="ppsA"
                     /locus_tag="PKV97_04695"
     CDS             5630..7996
                     /gene="ppsA"
                     /locus_tag="PKV97_04695"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNB05208.1"
                     /translation="MTRYVIPFNELRMSDVEKVGGKNASLGEMISQLPSSVRVPGGFA
                     TTAEAYREFLAHDGLADRINAALDALDVDDVDALAKTGAQIRQWIVDLPFPAKLEAEI
                     KTAYDAITAEGEGSFAVRSSATAEDLPDASFAGQQETFLNIHGYENILHAMKEVFASL
                     YNDRAIAYRVHKNFAHADVALSAGVQRMVRSDSGASGVMFSIDTESGFDQVVFITASY
                     GLGETVVQGAVNPDEFYVHKPTLALDKPAIIRRNLGSKLIKMVFTDKAVAGKSVRTVD
                     VPEADRNRFSLTDEDVLELARYAVIIEKHYGRPMDIEWGKDGVDGKLYILQARPETVK
                     SQDSGLVMEKYRLKQYGKALTHGRAIGQKIGAGTVRIVGDASEMNRVQAGDILVTDMT
                     DPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAIVGCGNATDVLHEGESVTVSCA
                     EGDTGYVYRGKLEFEVITTDMGNLPEIPVKIMMNVGNPELAFEFAQIPNGGVGLARLE
                     FVINNMIGIHPKAILDIGQVPASLRDEITRRSRGYATPKQFFIEKLVEGVATIAAAFY
                     PKPVIVRMSDFKSNEYRKLLGGEIYEPEEENPMLGFRGASRYIAHSFRDCFEMEVAAM
                     KKVRNELGLTNVQIMIPFVRNLEEAAGVVDLLAEHGLKRGANDLKLIMMCEIPSNAIL
                     AEQFLQYFDGYSIGSNDLTQLTLGLDRDSGLVAHSFDERDPAVKQLLSMSIQAANKLG
                     KYVGICGQGPSDHPDLAEWLMDEGIQTISLNPDTVVDTWLKLAAHGKR"
     gene            8145..11900
                     /locus_tag="PKV97_04700"
     CDS             8145..11900
                     /locus_tag="PKV97_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB05209.1"
                     /translation="MSGLRIESSVEVPARAGHGDVPPAPPPASVALADLERRVARQAQ
                     MLSVLSTLQRGMLVGRSSADTFSAMLDQLVALTGSEYGFIGEVLHDEAGESFLRMHGL
                     SDISWSPESRALFERMRTGGFEFHNLDTLFGSVVRTATPVICNAPAGDPRGAGVPPGH
                     PELRAFLGLPLFHGGQLIGMVGLANAAQGYDHALIVELEPMLVTCASMIIALRTEQDR
                     KAARRALEESERHFRELANSKSALIWTSDADGGCNYFNDTWLQFTGRTLEQSQGTGWV
                     ADVHADDLEGCLDTWQRAFARREPFRMEYRLRHAAGGYRWISDEGNPRRDADGAFVGY
                     IGYCYDITERKRGDLALDALAARYARLSGEGFYEAVCRHIVEALDLDIAFVGQLCADG
                     AHIEVRAGWGEGAAIGPFRYALADSPCDGTIGLGACIYPRGVHARFPRDRELVRMGIE
                     AYAGMPLFDCDGGVLGLIVALRRRPFDDEAAVRTLLEIFDDRVAAELARERAERVLNG
                     RIAFERLVGRISSDLILASPAELDLHLHRALGELGRFAEADRAYVFQVSEDGSLLDNT
                     HEWCAEGVEPQIHKLQGLPFDDSLLFLRTIKQLEIVDYPSVAALPAEAEADRVLLGAQ
                     SIRSVLGVPMVVDGQVRGVIGLDAVRSERTWTDEDKTLMTLVGNAFSGVIERKRAHDG
                     LQASEARYRSVVESVREVIFQLDGRGRWTFLNKAWADITGYALADSLGRHFLAHVHAD
                     HRDQHGEMIEQVLAGELESCTHAARYRCAGGGFRWLEMSARRAFDAQGRPAGLSGTLT
                     DITQQKEHESRLEYIAHYDALTGLPNRVLLSDRMQRGMAQARRRGQQLALAYIDLDGF
                     KAINDTLGHQAGDQLLAVVAARMKAALREGDSISRLGGDEFVALLLDLPDVEACELLV
                     RRLLGAVSQAVQLAGQEVKVSASIGLTLYPQAEEVDADQLLRQADLAMYQAKLAGKNR
                     YHLFDTAHDRSQRGRHQTLAAIRGALRTGEFRLLYQPKVNMRSGAVVGAEALIRWRHP
                     RQGELEPGGFLPVVADHPLAVEIGDWVIEQALAQVAAWQHAGLFLPVSVNVGARHLQA
                     ADFMPRLQAALARHPDLGPGALELEVLETSALESIAQVTRIVEACARIGVSFALDDFG
                     TGYSSLAYLKRLPAARLKIDQLFVRGMVEDAEDLAILKAIMGLADAFKREVIAEGVEE
                     VEHGSLLLQLGCEVAQGYGIARPMPAEEIPAWVANWSPPAAWRQARA"
     gene            complement(11991..>12292)
                     /locus_tag="PKV97_04705"
     CDS             complement(11991..>12292)
                     /locus_tag="PKV97_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HNB05210.1"
                     /translation="TGLISMIGKSERGPVAIEAIKDNQSAYLMAVGGAAYLVSKAIKE
                     AKVVGFADLGMEAIYEFTVQDMPVTVAVDAKGTSVHNTGPQEWQAKIGKIPVVVA"
BASE COUNT         1845 a         4093 c         4317 g         2037 t
ORIGIN      
        1 gtgctggtgc cgctcgtggt gcgcgacgag gtgccgtcgg tgctgctcac ccggcgcacc
       61 gaccacctgc accatcatcc gggcgagatc agctttcccg gcggccgcct cgaggacgac
      121 gacccctcgc cggtgcatgc cgcgctgcgc gagacggaag aggagatcgg gctcgcccgc
      181 cgtcacgtcg agctgatcgg cgcgctgccc gactacttca ccggtaccgg cttcagggtg
      241 accccggtgg tcggcgtggt gcatccgccg ctcgaactca cgctcgacgc cttcgaggtc
      301 gcggaggcct tcgaggtgcc gctcgcgcac ttcctcgatc cgcgcaacca tcaggagcac
      361 agcatcctgc acgaggggcg gatgcgacgc ttccacgcca tgccctggca cggctatttc
      421 atctggggtg cgaccgcggg gatcctgatg tcgctgtatc gcctgctgaa cgaggaaggc
      481 tgagcgcggc caagggtttc cggcaggcgg cgcgcgcttg cctttgtgat atcttgccgc
      541 accgatgact cggctctgtg cccggggcct tttcggcccg cgacgtcctg cctgaatgac
      601 cttcttttcc ttgatccttg cgctcgtcct cgaacagttg cgtccgctgc cggcgcgcgg
      661 gtggacggtg cgcggggtgc gcggcgtggt cggcggaatg cgagggcgct tcgagggcgg
      721 ctcggcggcg cagggacggc tcgcgtggct cctgctggtt cccgccgccg ggttcgcgag
      781 cgcgctcgcc ttctggctgc tgtgggagat gcatcccgcg ctggccttcc tgttcaacgt
      841 ggtggcgctc tacctctgca tcggcttccg ccacgacacc cgcttcttct cggccatcca
      901 catggcgctt cgcctcggcg agcccgaggg cgcgcgcagc ctgctcgagg attggcgggg
      961 gacccgccac gagggggcct ccgcgagcga gatcgcccgc ctcgcgatcg aggaggcgct
     1021 ggtcgtctcg caccgccggc tgttcggcgt gatgttctgg ttcgtgctgc tacccggccc
     1081 gagcggcgcg gtgatgtacc ggctcgccgg actgctggcc gaggagtggg ggcgcccgga
     1141 tgggggcgac gcgggcttcg gcggcttcgc gctgcgcgcg cgcgaggcga tcgagtggct
     1201 gccggcgcgg atcaccgcga tcgttttttc catcatgggc aatttcgagg atgcgatcta
     1261 ctgctggcgc tcgcaggcgg tgctgtggcc ggagaggtcg tcggctatac ttatcgctag
     1321 tggtggtggc gcgctgggcg tgcggctcgg catgccggtc catgtggccg ggggcatcgt
     1381 ggatcggccc gaactggggc tggggagcga ggcaggcgtc gatcacatgc aaaacacggt
     1441 gagcctgcta tggcgggctc tgttcgtgtg tctgttcctg ctcttcctgc tcgcggctgc
     1501 gggctgggtg gggcggtgac aagtctaatc tcaacaaggg agtgggttat ggcaaatcgt
     1561 gaagtcgtcg ttctgagcgc tgttcgttcc gccgtgggtg gtttcggtgg ctcgctgaag
     1621 gacatggagc cgtccgatct cggcgcggtg gtgatcaagg aagcgatcgc ccgcgcgggt
     1681 gtggacccga aggcggtttc gttcgcatcc gtcggccact gcatcccgac cgattcccgc
     1741 tatgcctacg tctcgcgcgt ggccacgatc aacgccggca tgtcgatgga ttcggtcgcc
     1801 ttccaggtca gccgtctgtg cggctcggcg cagcaggcca tcgtcagctc ggcccaagcc
     1861 atcatgctgg gcgatgccga cttcgccatc ggcggcggcg tcgaagtgat gtcgcgcggt
     1921 acctacctgc tgcccgccct gcgcagcggt gcgcgcatgg gcgacaccaa ggcgatcgac
     1981 tcgatggtcg ccgtgctgac cgacccgttc ggcgtcggcc acatgggcat caccgccgaa
     2041 aacctgtgca ccaagcacgg catcacccgt gaagagcagg atgccttcgc gctcgagtcg
     2101 cagaagcgcg ccgccgcggc gatcgccgag ggccgcttca agggccagat cgtgccgatc
     2161 accttcgaga cccgcaaggg caccgtcgtg ttcgacaccg acgagcatcc gcgcatgacc
     2221 accatggaag cgctgggcaa gatgaagccg gcgttcaaga aggatggttc ggtcaccgcc
     2281 ggcaacgcct cgggcatcaa cgacgccgcc gccttcctgg tgctggccga tgccgccaag
     2341 gcggctgcgg gcggccacaa gccgatcgcg cgcctggtgt cctacgcgat cgccggcgtg
     2401 cccaacgacg tgatgggcga aggtccgatc cccgccacca agctcgcgct gcagcgtgcc
     2461 ggcctgaagc tcgaccagat cgacgtgatc gagtccaacg aagccttcgc cgcgcagtcg
     2521 atcacggtga accgcggtct cggcctcgac accgccaagg tcaacccgaa cggcggcgcg
     2581 atctcgctcg gccaccctgt cggcgcgacc ggctcggtga tcatcaccaa gctgctgcac
     2641 gagctgatcc gcaccaacgg ccgctatggc gtcgccacca tgtgcatcgg cggcggccag
     2701 ggcatcacca cggtgtggga acgcgtgtaa acgtcgtcct cgtcgcaaga aaaacccgcg
     2761 cggccgagag gctggcgcgg gttttttcgt ttcgggcgcg ctgaactggc aatcgcgact
     2821 tgcgtcgctc ctacggggcg gtctgggggt ctcgatcgcg acttgcgtcg ctcccacggg
     2881 gggcctcgat cgcgacttgt gttgctcctc cggggggctg cggagctgtg cgggttttgt
     2941 gggagcgacg cgagtcgcga tcttcagtcc gactccttct ccaggcgcac cgacgccgag
     3001 ttgatgcagt agcgcagccc ggtgggctgc gggccgtcct cgaagacgtg gccgaggtgt
     3061 gcgccgcact ggtggcaaag gacctcggtg cggaccatga agtggctgcg gtctaccgcg
     3121 gtctcgacgt tgtcgggctc ggctgcggtc cagaagctcg gccagccgca gccggcgtcg
     3181 aacttgtgct cggagcggaa gagcggggca ccgcagcaca cgcagttgta ggcgcccttg
     3241 tcccagacgt cccagtattc gccggtgaag gcacgttcgg tgcctttctc gcgggtgacg
     3301 cggtactgca ggtcggagag ctggcggcgc cattcggcgt cgggtttgtc gacttcgcgg
     3361 gccatgggaa cctcgtgcgg gatcagtgga ggtggcgcgg cgcctgcacg ccctgttcct
     3421 cgggcatctc ggcatgcacg acctcgccct cgggcgaggg gtagagcggg gcgccgcaat
     3481 cgtcgcagta ctcgagcggg aagtggtgct cgagcgccac gacgtcggtg agaccggatt
     3541 cgcgcaatac ggtttcgatc tgcgcgacgc agtcggtggt ctcgtcctcg gcccccaaca
     3601 gcggccacac caccccgtgc acgacctcgt cgcggccgcg cacggtgaag ccgatgcggt
     3661 attcctcgag agtgcgctcc cagaacggcg cgaccaccgc gcgcaggttg gcggcaggtg
     3721 tttccagcgt ggtggcgagg aaggcgaccg aggcgcgcac cgagtagggc cggctgtcct
     3781 tgtcggcggc gcggctggcg gcgaagtagg agtcgggcag cacgaggtcg accgcgcagc
     3841 cgggcagcag cggggtcagg caggcgccac cctgggcgcg ccactgctcg agcgcgccgt
     3901 cgcgggtgcc gtccggctcc tgccagcgga acagcgcggc gccgcgcggc acggccaccg
     3961 cggcgagcag gtagcgggtg tcggagagga actgcgcggt ctccggcatg ccatcggtgt
     4021 cgatgtgcag gtcgaggttg tcggccgccg caccgcagat caccttggcg aactccgcgg
     4081 tggcgcagta gccctggggc agctggtcgg ggctgaagag gaagtcggcc accgacagct
     4141 tggccccctt ggcgagcacg tgggcctgca ggtgcacgcg caggttggcg agcactgcgg
     4201 gcgggatgtt ggcggcgggg atgcggtagc gcgaccaggc cagcaccggc gcggcgatca
     4261 gcacgacgtc gtgctcggcg aggctgccgg cggcgcattc ggcgcgcgac tcgatgttgt
     4321 cggcgagctc gtcgtaggcg cgcgcgtcgg cgctgtagag gtggtcgagc gcggcgttga
     4381 gcacttcctc gtcgttgtcg gtcagggtct cgtcgatcag cctggcgagc tgcgtctccc
     4441 agtagtggtc ctcgatccgg ctgccggact gggccagccc gccggcgagc cagacgagtt
     4501 gttcggcagt gcggccgaga ccgccgcggc gcttgaggcg cgttcttttc atgtcgggtt
     4561 cctgttgcgc tgttcggggt gccggcttgg gcggcacgca tgaccgcgta ttctaccaat
     4621 gccgcgggaa aacgccggtc gaagcgcccg gcgcgcgctc agaaaccggg gcggtcgacc
     4681 cggatggtct gcaggatggt ggcggagatc tcctcgatcg acttggtggt ggagtcgagg
     4741 cagcggatgt tctcgcggcg catcagcttg tgcgcggcgt cgatctcgta gcggcagttc
     4801 tccagcgagg cgtagcggct gttggggcgg cgttcctggc ggatctgcga caggcgctcg
     4861 ggggcgatgg tgaggccgaa gagcttggtg cggtacttgt gcagctcgcc ggggagcttg
     4921 ttgcgctcga agtcctcggg gatcagcggg tagttggccg ccttgacgcc gaactgcatc
     4981 gccaggtaga ggctggtggg ggtcttgccc gagcgcgaca cgccgaccag gatcacgtcg
     5041 gcctcggcga gctcgccctc gctggagatg ccgtcgtcgt gcgccatggc gaagttgatc
     5101 gcctcgatgc ggtacttgta gttgaggctg tcggcgatgc catggaagcg gcccacggca
     5161 tgggtggagc gctggccgag ctcggcttcg agcgggccga tgaagcgctc gaagaggtcc
     5221 aggaagagcg cgtcggattt gtgcagtgcg tcaacggtgg cctgattgac cagcgtgctg
     5281 aatacgatcg ggcgcacgcc gtcctcggcc gcagcctcgc ggatctgcgt cgcgcagtcg
     5341 atcgccttgt cgatgtcgtc gatgaaaggg gcgcgcacct ggcggaagcg cgcctcggga
     5401 aactgcgcca acaggctgtg gccaagggtt tcggcggtaa tgccggtgcc gtcggagacg
     5461 aagaagacgg tgcggcgggc gatgtcgttc atgatttcgg accttgcggc cccgaccagg
     5521 ggtgcggggc gtgtattggg gcaaaccctg tgatgcggca cagcacaaaa aatcgtacac
     5581 tgccgttcgc gatattttgc tttttcccca acatttgcgg aagccatcca tgacacgcta
     5641 cgtgatccct ttcaacgaac tgcgcatgtc cgacgtcgag aaggtcggcg gcaagaacgc
     5701 ctcgctcggc gagatgatca gccagctgcc ttccagcgtc cgtgtcccgg gcggcttcgc
     5761 caccaccgcc gaagcctatc gcgagttcct cgcccacgac ggcctggccg accgcatcaa
     5821 cgccgcgctt gacgcgctcg acgtggatga cgtcgatgcc ctggccaaga ctggcgcgca
     5881 gatccgccag tggatcgtgg acctgccctt cccggccaag ctcgaggccg agatcaagac
     5941 cgcctacgat gcgatcaccg ccgagggcga gggcagcttc gcggtgcgct cttcggcgac
     6001 cgccgaggac ctgccggatg cctccttcgc cggccagcag gaaaccttcc tcaacatcca
     6061 cggctacgag aacatcctgc atgcgatgaa ggaagtgttc gcgtcgctgt acaacgaccg
     6121 cgcgatcgcc taccgcgtgc acaagaactt cgcccacgcc gacgtcgcgc tgtcggccgg
     6181 cgtgcaacgc atggtgcgtt ccgattcggg cgcctcgggc gtgatgttct cgatcgacac
     6241 cgaatcgggc ttcgatcagg tggtgttcat caccgcctcc tatggcctgg gcgagaccgt
     6301 ggtgcagggc gcggtgaacc cggacgagtt ctacgtccat aagcctacgc tcgcgctcga
     6361 caagccggcg atcatccgcc gcaacctcgg ctccaagctc atcaagatgg tgtttaccga
     6421 caaggccgtc gccggcaagt cggtgcgcac ggtcgatgtg cccgaggccg atcgcaaccg
     6481 cttctcgctg accgacgagg acgtgctcga gctcgcccgc tacgcggtga tcatcgagaa
     6541 gcactacggc cgcccgatgg acatcgagtg gggcaaggac ggcgtcgatg gcaagctcta
     6601 catcctgcag gcgcgtcccg agacggtgaa gagccaggat tccggcctgg tgatggagaa
     6661 gtaccgcctc aagcagtacg gcaaggccct cacccacggc cgcgcgatcg gccagaagat
     6721 cggtgccggc acggtccgca tcgtcggcga cgcctccgag atgaaccgcg tgcaggccgg
     6781 cgacatcctc gtcaccgaca tgaccgaccc caactgggag ccggtgatga agcgcgccag
     6841 cgcgatcgtc accaaccgcg gcggccgcac ctgccacgcc gcgatcatcg cgcgcgagct
     6901 gggcatcccg gccatcgtcg gctgcggcaa cgccaccgac gtgctgcacg aaggcgaatc
     6961 cgtcaccgtg tcctgcgccg aaggcgacac cggctacgtg tatcgcggca agctggagtt
     7021 cgaggtcatc accaccgaca tgggcaacct gcccgagatt ccggtgaaga tcatgatgaa
     7081 cgtgggcaac cccgagctcg ccttcgagtt cgcgcagatc cccaacggcg gcgtcggcct
     7141 ggcgcgcctg gagttcgtca tcaacaacat gatcggcatc caccccaagg ccatcctcga
     7201 catcggtcag gtgccggcca gcctgcgcga cgagatcacc cgccgctcgc gcggctatgc
     7261 gacgcccaag cagttcttca tcgagaagct ggtcgagggc gtggccacca tcgccgcggc
     7321 cttctatccg aagccggtga tcgtgcgcat gtccgacttc aagtcgaacg agtatcgcaa
     7381 gctgctcggc ggcgagatct acgagccgga ggaagagaac ccgatgctcg gcttccgtgg
     7441 cgcctcgcgc tacatcgcgc acagcttccg cgactgcttc gagatggaag tcgccgcgat
     7501 gaagaaggtg cgcaacgagc tcggcctcac caacgtgcag atcatgatcc ccttcgtgcg
     7561 caacctcgag gaggccgcag gggtggtgga cctgctcgcc gagcacggtc tcaagcgcgg
     7621 cgcgaacgac ctcaagctga tcatgatgtg cgagatcccg tccaacgcca tcctggccga
     7681 gcagttcctg cagtacttcg acggctactc gatcggctcg aacgacctca cccagctcac
     7741 cctgggcctg gaccgcgact cggggctggt ggcgcattcc ttcgacgagc gcgatccggc
     7801 ggtcaagcag ctgctgtcga tgtccatcca ggcggccaac aagctcggca agtacgtggg
     7861 catctgcggc caggggccgt cggaccaccc cgacctggcc gagtggctga tggacgaggg
     7921 catccagacc atctcgctga acccggacac cgtggtcgac acctggctca agctcgccgc
     7981 acacggcaag cgctgaacgc cgcttcggca cgctgtcgta tcgagggcgt cgaccgcgag
     8041 ggtcggcgcc cttttttatg catccgggcg ttgcagtcat gccgctggaa agagcttccc
     8101 gctaacatgc gcgcttgtcg tgcatgcgga ttcgagggga tcggatgtcg ggcttgagga
     8161 tcgagtcgtc ggtggaggtg cccgcccggg caggacatgg ggatgtgccg ccggcgccac
     8221 cgcccgcatc cgtcgcgctc gccgacctcg agcgtcgcgt ggcgcgccag gcgcagatgc
     8281 tgtcggtgct gtccacgctg cagcgcggca tgctcgtggg gcgttcgtcc gccgacacct
     8341 tctccgcgat gctcgaccag ctggtggcct tgaccggcag cgagtacggc ttcatcggcg
     8401 aagtgctgca cgacgaggcc ggcgagtcct tcctgcgcat gcacggactc agcgacatct
     8461 cctggagtcc cgagtcgcgc gccctgttcg aacgcatgcg caccggcggt ttcgagttcc
     8521 acaacctcga caccttgttc ggttcggtgg tgagaaccgc cacgccggtg atctgcaatg
     8581 cgcccgcggg cgatccccgc ggcgcagggg tgccgcccgg ccaccccgag ctgcgcgcct
     8641 ttctcggcct gcccttgttc cacggcgggc agctgatcgg catggtcggc ctggcgaacg
     8701 cggcgcaggg ttacgaccac gccctgatcg tcgagctgga gccgatgctg gtcacctgcg
     8761 cgagcatgat catcgcgctg cgcaccgagc aggaccgcaa ggccgcacgt cgcgccctgg
     8821 aggagagcga acgccatttc cgcgagctcg ccaacagcaa gtcggcgctg atctggacct
     8881 cggacgcgga cggcggatgc aactatttca acgacacctg gctgcagttc accggccgca
     8941 cgctggaaca atcgcaaggc acgggctggg tggccgacgt gcatgcggac gacctggagg
     9001 gctgcctcga cacctggcaa cgagccttcg cccgccgcga gcccttccgc atggaatacc
     9061 gcctgcgcca cgccgcgggc ggctaccgct ggatttcgga cgagggcaat ccgcgccgcg
     9121 acgcggacgg cgccttcgtc ggctacatcg gctattgcta cgacatcacc gagcgcaagc
     9181 gcggcgacct cgcgctcgat gcgctcgcgg cgcgctacgc gcggctgtcg ggcgagggct
     9241 tctacgaggc cgtgtgccgg cacatcgtcg aggcgctcga tctggacatc gccttcgtcg
     9301 gccagctgtg cgcggacggc gcgcacatcg aggtgcgcgc gggctggggc gagggcgcag
     9361 cgatcgggcc gttccgctat gcccttgccg actccccctg cgatggcacg atcgggctgg
     9421 gcgcctgcat ctacccgcgg ggggtgcatg cgcgctttcc gcgcgaccgc gaactcgtgc
     9481 gcatgggcat cgaggcctac gccggcatgc cgctgttcga ttgcgatggc ggcgtcctcg
     9541 gcctcattgt cgcgctgcgt cgccgcccct tcgacgacga ggcggcggta cgcaccctgc
     9601 tggagatctt cgacgaccgc gtcgcggccg agctggcgcg cgagcgcgcg gagcgcgtgc
     9661 tgaacggacg gatcgccttc gagcgcctgg tcgggcggat ctcgtccgat ctgatcctcg
     9721 cctcgccggc cgagctcgac cttcatctcc atcgggcgct cggcgaactt gggcgcttcg
     9781 ccgaggcgga tcgcgcctac gtgttccagg tgtccgagga cggttccctg ctcgacaaca
     9841 cccacgagtg gtgcgccgag ggcgtcgagc cgcagatcca caagctgcag ggcttgccct
     9901 tcgacgactc gctgctcttc ctgcgcacga tcaagcagct cgagatcgtc gattacccca
     9961 gcgtcgccgc gctgccggcc gaggccgagg ccgaccgcgt gctgctcggg gcgcagtcga
    10021 tccgttcggt gcttggcgtg ccgatggtcg tggacggcca ggtgcgcggc gtcatcgggc
    10081 tcgacgcggt gcgcagcgag cgcacctgga ccgacgagga caagaccctg atgaccctgg
    10141 tgggcaatgc cttcagcggg gtcatcgagc gcaagcgcgc ccacgacggc ctgcaggcga
    10201 gcgaggcgcg ctaccgctcg gtggtcgaga gcgtgcgcga ggtcatcttc cagctcgacg
    10261 ggcgagggcg ctggaccttt ctcaacaagg cgtgggccga catcaccggc tacgcgctcg
    10321 cggatagcct cggccgccac ttcctcgccc acgtgcacgc ggaccatcgc gaccagcacg
    10381 gcgagatgat cgagcaggtg ctcgcaggcg agctcgagtc ctgcacccac gcggcacgct
    10441 accgctgcgc cggcggcgga ttccgctggc tggagatgag cgcgcggcgc gcgttcgacg
    10501 cgcaaggccg gcccgcgggc ctttccggga cgctgaccga catcacccag cagaaggagc
    10561 acgagtcgcg cctggaatac atcgcgcatt acgacgcgct caccgggctg cccaaccgcg
    10621 tcctgctcag cgaccgcatg cagcgcggga tggcgcaggc gcgccggcgc ggtcagcagc
    10681 tcgcgctcgc ctacatcgat ctcgacggct tcaaggccat caacgatacc ctcggccacc
    10741 aggccggcga ccagttgctc gcggtggtgg cggcgcgcat gaaggcggcg ctgcgcgagg
    10801 gcgacagcat ttcgcgcctg gggggggacg agttcgtcgc cctgctgctc gacctgcccg
    10861 atgtagaggc ttgcgaactg ctcgtgcgcc gcctgctcgg cgcggtctcg caggcggtcc
    10921 agcttgcggg gcaggaggtg aaggtctcgg cgagcatcgg cctgaccctg tacccgcagg
    10981 cggaggaggt cgacgccgac cagttgctgc gccaggccga cctcgccatg taccaggcca
    11041 agctcgccgg caagaaccgc taccacctct tcgacaccgc gcacgaccgc tcccagcgcg
    11101 ggcgccacca gaccctggcg gcgatccgtg gcgcgctgcg cacgggcgag ttccgcctcc
    11161 tgtaccagcc caaggtgaac atgcgcagcg gcgcggtggt gggtgccgag gcgctgatcc
    11221 gctggcggca tccgcgccag ggcgaactcg agccgggcgg cttcctgccc gtggtggcgg
    11281 accacccgct cgcggtcgag atcggcgact gggtgatcga acaggcgctc gcccaggttg
    11341 ccgcctggca gcacgcggga ttgttcctgc cggtgagcgt gaacgtgggc gcgcgtcacc
    11401 tgcaggcggc ggatttcatg ccgcgcctgc aggcggcgct ggcgcgccac cccgatctgg
    11461 ggccgggcgc gctggaactg gaggtgctgg agacgagcgc gctggagagc atcgcccagg
    11521 tcacgcgcat cgtcgaggcg tgcgcacgca tcggcgtgtc cttcgcgctc gacgacttcg
    11581 ggacggggta ttcctcgctc gcctatctca agcggctacc cgcggcgcgt ctcaagatcg
    11641 accagctgtt cgtgcgcggg atggtggagg acgccgagga cctcgccatc ctgaaggcga
    11701 tcatggggct ggccgatgcc ttcaagcgcg aggtgatcgc cgagggggtc gaggaggtcg
    11761 agcacggcag cctcttgctg caactgggct gcgaggtggc gcagggctac gggatcgcgc
    11821 ggccgatgcc ggcggaagag attccggcct gggtggcgaa ctggtcgccg cccgcggcgt
    11881 ggcggcaggc gcgggcctga gcccgcgccc tgcgtcctcc acccgcgccc cggcccctcg
    11941 atgcgggcgc tccgggaagc gctcgggggc agggcgcgcg tcgagggcgc tcaggcgacg
    12001 acgaccggga tcttgccgat cttcgcctgc cactcctgcg ggccggtgtt gtgcacgctg
    12061 gtgcccttgg cgtcgactgc gaccgtcacc ggcatgtcct gcacggtgaa ttcgtagatc
    12121 gcctccatgc cgaggtcggc gaagccgacg accttggctt ccttgatcgc cttcgacacc
    12181 aggtaggccg cgccgccgac cgccatcaga taggccgact ggttgtcctt gatggcctcg
    12241 atcgcgaccg ggccgcgctc ggacttgccg atcatcgaga tgaggccggt ct
//
LOCUS       DAOQNN010000047        12207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2302_length_12207_cov_10.617758, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000047 DAOQNN010000000
VERSION     DAOQNN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12207
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2302_length_12207_cov_10.617758"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..643)
                     /locus_tag="PKV97_04710"
     CDS             complement(35..643)
                     /locus_tag="PKV97_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HNB05211.1"
                     /translation="MREALWSLALLLAAYAAFEFLRALRLPREGASGLAVGLQPVTGT
                     RTAAAAAAHDARDDEDDDFLAYAPLPSSVSSAAPAGAAPSAPTSAPAASSGAAEAEAA
                     AAARFELELENSRLRQALQAQRDLVALQQTEIDRLVEDIAALRDELDAQASSVASSPE
                     YSEAMVLARQGLNAEMIAVRCGITLAEAELVLALGRGAEGHG"
     gene            808..1587
                     /locus_tag="PKV97_04715"
     CDS             808..1587
                     /locus_tag="PKV97_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNB05212.1"
                     /translation="MELDPDTLLFWLKKLVAVLALPPVLPLLPIVIGLITLRRRPRLG
                     LALAWAGVALNLLLIFPPSVGWLVARVEYPQMLDRARAREAQAIVVLGAGKRTHAPEF
                     GGETVNRLALERLRYAAALARETRLPVLVSGGAPAGDTPEAYLMEALLRDEYGVPPRW
                     VESASRDTRENARFSAVHLQAAGVKRILLVTHAMHMERARPEFEAAGLDVIPAPTAWL
                     GGGDSPGDAQPLPFAPSQNTAYAAWFALHELLGRLAYRWSR"
     gene            complement(1729..2952)
                     /gene="nqrF"
                     /locus_tag="PKV97_04720"
     CDS             complement(1729..2952)
                     /gene="nqrF"
                     /locus_tag="PKV97_04720"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HNB05213.1"
                     /translation="MNTEIILGIGMFTAIVLALAVFIIAARSKLVSSGDVTIDINGEK
                     KIVVPAGGKLLQTLAGNGLFLPSACGGGGTCAQCKCVVMEGGGSMLPTEESHFTKRDA
                     KEGWRLSCQTPVKQDMKIEVPEEVFGVKKWECTVESNPNVATFIKELTLRLPEGEDVH
                     FRAGGYVQLECPPHVVNYKDFDIQDEYRGDWDKFNLWRFVSKVDETTIRAYSMANYPD
                     EKGVVKFNIRVASPPPGRDDVPPGKMSSWVFNLKKGDKVTVYGPFGEFFARDTDHEMV
                     FVGGGAGMAPMRSHIFDQLKRLHSKRKISFWYGARSMREAFYVEEFDKLAAENPNFKW
                     HLALSDPLPEDNWTGYTGFIHNVLYENYLKDHPAPEDCEFYMCGPPMMNAAVIKLLLD
                     LGVERENIMLDDFGG"
     gene            complement(2968..3576)
                     /gene="nqrE"
                     /locus_tag="PKV97_04725"
     CDS             complement(2968..3576)
                     /gene="nqrE"
                     /locus_tag="PKV97_04725"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HNB05214.1"
                     /translation="MEHLISLFVRSVFIENMALSFFLGMCSFIAISKKVETAIGLGVA
                     VVVVQAITVPANNLMYNLLLREGALAWAGLPEVDLSFLGLLSYIGVIAAIVQILEMLL
                     DKYMPSLYNALGIFLPLITVNCVIMAGSLFMVERDYTLAESAVYGVGSGASWALAIAL
                     LAGIREKLKYSDVPEGLQGLGITFITIGLMSLGFMSFSGVQL"
     gene            complement(3578..4246)
                     /locus_tag="PKV97_04730"
     CDS             complement(3578..4246)
                     /locus_tag="PKV97_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HNB05215.1"
                     /translation="MSKPTVKEVLLNPILNNNPIALQILGICSALAVTSNLKTAVVMS
                     IALTLVTACSSMFISMIRSQIPSSIRMIVQMVIIASLVIVVDQLLRAYAFSLSKQLSV
                     FVGLIITNCIVMGRAEAFAMQNPPLLSFMDGIGNGLGYSAMLITLGVIRELFGAGKLF
                     GIEIIALAKDGGWYVPNGLLLLPPSAFFLIGLIIWGLRTWKKEQVEKPAFRMAPQVVE
                     KEAY"
     gene            complement(4249..5034)
                     /locus_tag="PKV97_04735"
     CDS             complement(4249..5034)
                     /locus_tag="PKV97_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HNB05216.1"
                     /translation="MSNRESTSRTLMVALAVSLVSSVFVAGAAVSLKPVQVENRLLDK
                     QRSILSIAGLGDAEMSSREVKDLFAGRIKARVVDLQTGEYTDAHDAQLFDPLKAARDP
                     KLSDALDGEEDIALIKRRERFTTVYMVEKDGKLESLILPVRGYGLWSTLHGFVALKGD
                     LNTVVGLGFYQHAETPGLGGEVDNPKWKALWPGKVLFDAQGKPAIHIVKGGVDPASPD
                     AKHQVDGLAGATLTSNGVDRLLQFWLGEQGFGPYLAKLRTQGV"
     gene            complement(5024..6238)
                     /locus_tag="PKV97_04740"
     CDS             complement(5024..6238)
                     /locus_tag="PKV97_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HNB05217.1"
                     /translation="MSLRSWLDSIEHHFEKGGKYEKYYALYEAVDTALYKPPLVTRNT
                     AHVRDGLDLKRMMITVWLCTFPAMFFGMWNVGYQANTILAGSPELMAAQEGWRIALTS
                     ALAGLDPASLWANFLHGATYFLPIYLTTFIVGGFWEVLFASIRKHEVNEGFFVTSVLF
                     ALTLPPDTPLWQVALGISFGVVIAKEVFGGTGKNFLNPALAGRAFLYFAYPAQMSGDA
                     VWTAVDGFTGATMLSLGAAGGIEAVTGAGIDWMRAFLGTVHGSIGETSTLAILIGGAV
                     LLVMKIASWRIVSGVMLGMVATSLLFNAIGSDTNPMFAVPWHWHLVMGGFAFGMMFMA
                     TDPVSASMTNTGKWIFGAVVGIMTVLVRVVNPAFPEGIMLAILFGNLCAPLIDHFVVT
                     ANIKRRLARNVQ"
     gene            complement(6241..7581)
                     /locus_tag="PKV97_04745"
     CDS             complement(6241..7581)
                     /locus_tag="PKV97_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HNB05218.1"
                     /translation="MIRIKRGLDLPIAGAPEQRIEAGRPVRSVAVVGFDYHGMKPTMA
                     VQVGDRVKLGQVLFSDKKTPGVVFTAPGAGTVSAIHRGEQRVLQSVVIDLDGDDAVEF
                     TRYTDAELDRLDDATVRSKLIDSGLWTALRTRPFSKVPAIDAKPHSIFVTAIDTHPLA
                     ADPTVVIAEHTDDFARGVRVLARIAPVVVCHADGTRMPCSGLADVRTETFAGPHPAGL
                     AGTHIHFLDPVHAEKSVWSLNYQDAIAIGKLFSTGRLWTERVVSIAGPMAAKPRLVRT
                     RVGANLDELVAGELKTGKKVRVISGSVFGGRTSRGACAFLGRYHLQVSCLEEGSEREM
                     LHYLRAGANKHSVMNLYISKLSPGRLFDFTTSTNGSPRAMVPIGNYEEVMPLDILPTQ
                     LLRALIVGDTDMAQKLGCLELDEEDLALCSYVCAGKYEYGPILRDNLTRIEKEG"
     gene            complement(7933..9132)
                     /locus_tag="PKV97_04750"
     CDS             complement(7933..9132)
                     /locus_tag="PKV97_04750"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNB05219.1"
                     /translation="MSKQIQDAYIVAAVRTPVAKRNGAFRAVRPDDLLARVLREVVAK
                     VPNLDAREIGDVVTGCAMPEAEQGMNVARIGLLLAGLPDSVPGITINRFCASGLQAVA
                     DAASRIRLGEADVMIAAGTESMSAMPQIMGNKVSLNPEIFARAENLSIAYGMGLTAEK
                     VAQQWKVSREDQDAFAVESHRRACAAIAAGQFADEILPYAVKSYAPGVDGTVRITERL
                     VEHDEGPRPDAAADRLGKLKPVFAARGSVTAGNSSQMSDGAAAVLLMSETALKRYDVT
                     PIARFSSFAVAGVPPEVMGIGPVEAIPRALARAGVALADIGWTELNEAFAAQALAVMR
                     QLGMDPAKVNPLGGAIALGHPLGATGAIRTATLMAAMQRDPGLRYGMISMCIGTGMGA
                     AGVFERV"
     gene            complement(9147..11528)
                     /locus_tag="PKV97_04755"
     CDS             complement(9147..11528)
                     /locus_tag="PKV97_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HNB05220.1"
                     /translation="MSRLIIRKVAVLGAGVMGAQIAAHCANADVPVILFDLPAKEGPA
                     NGVVDKALAGMKKLDPAPFAAKDRAQYVESANYGSDLARLGECDLVIEAIAEKMEWKL
                     DLYAKVAPHLKAGAIFASNTSGLSIEALATGMPADRRSQFCGIHFFNPPRYMPLVELI
                     PIAATDAALLDGLEAWLTTHLGKSIVRAKDTPNFVANRVGVFSILAVLHHTARLGLAF
                     DEVDLLTGPRIGRPKSATFRTADVVGLDTLVHVLGTMQATLPDDPWCAHFRTPEWLQA
                     LIAKGALGQKTKCGIYRKQGRQIQVLDLHLQDYRESGGEVLPEVAEILKLKNPAEKFA
                     QLAAHPHPQAQFLWSIFRDVFHYCAVHLGDIADSARDIDLAMRWGFGWAQGPFETWQA
                     AGWRAVAEMIRDDIAHGRAMSAVPLPAWVFERDGVHAPAGSYSAAEDSLKPRSALPVY
                     QRQLYPDRVLGEAPDDRGETLWENAGVRLWRRADQDARIGIVSITSKMHAVGEEVMDG
                     LLEAIARAETDLDGLVIWQPAPFAVGANLQQVGEACRAGQFELLERMVAKFQRTSMAI
                     KHAQVPVVAAVQGMALGGGCEFVMHAAHRVFALESYVGLVEAGVGLIPAGGGSKEFAL
                     QAHALAQRAAGGDVFPYIQNAFQTIAMATVAKSAQEAVQLGFGRTGDDILFNANEILY
                     AAIRRARALAESAWRPALVNPAVTVAGRNGIATCEMMLVNMAEGGFISAHDYRVAKAA
                     ATALCGGEVDTNRRVSEQWILEVERAQFVELLKTEKTQQRIAHMLETGKPLRN"
     gene            complement(11613..>12207)
                     /locus_tag="PKV97_04760"
     CDS             complement(11613..>12207)
                     /locus_tag="PKV97_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNB05221.1"
                     /translation="HELKGSLQFSGTAANLHPLTTGVSASADVELPDTFILSLSHKLD
                     SRWELLGDVSWTGWSSVDKVDIMRTSGLLLGTPLEKAQTLDTDFQDTWRVALGANYQL
                     NDAWKLKFGVAFDETPVKNPEKRLTSLPDNDRIWFSFGGQWKPSKSSALDMGVSYLYV
                     DDTKINNGNAADLSKGLVKGEYDSSVWILGAQYSMSF"
BASE COUNT         2018 a         4025 c         4199 g         1965 t
ORIGIN      
        1 agcgcggagc gggtggccgg aaagcgcggc agcttcatcc gtgcccttcc gcaccgcgcc
       61 cgagtgcgag gaccagctcg gcctcggcga gggtgatgcc gcagcgcacg gcgatcatct
      121 cggcgttaag cccctgcctg gcgagcacca tggcttcgga gtattcgggc gagctggcga
      181 cggacgacgc ctgggcgtcc agttcgtcgc gcaacgcggc gatgtcctcc accagacgat
      241 cgatctcggt ctgctgcagc gccaccaagt cccgctgcgc ctgcagtgcc tggcgcagcc
      301 gcgagttctc gagctcgagc tcgaagcgcg ctgcggcggc tgcctcggcc tcggcagcgc
      361 cggaagaggc ggcaggagcg gaggtggggg cggaaggcgc agcgcctgca ggcgccgcgg
      421 aggaaacgga ggaaggcaag ggtgcgtagg cgaggaagtc gtcatcctcg tcatctctcg
      481 cgtcgtgggc tgccgcggcc gccgccgtcc gcgtccccgt gacgggctgc agcccgacgg
      541 caagcccgga cgccccttcg cgcggcaggc gcagcgcgcg caggaactcg aacgcggcat
      601 aggccgccag caacagggcc agcgaccaca aggcctcacg catgagcggc gatcctccac
      661 gaggcacggt catccacgcg gaatcggcag cgatctcgca gcgggcgacg gcgggagttc
      721 aggagagggc gcatgagggc aacagaggct cgatgcaccg ataatacttg gccgcggcag
      781 ccgccgccaa acccgccccg accgaccatg gaactcgacc ccgacaccct gctcttctgg
      841 ctcaagaagc tggtcgcagt gctcgcgctg ccaccggtgc tgccgctgct gccgatcgtg
      901 atcggcctga tcaccctgcg ccggcggcct cggctcggcc tggcgctcgc gtgggcgggc
      961 gtggcgctga acctgctgct gatcttcccg ccgagcgtgg ggtggctggt ggcgcgcgtc
     1021 gaatacccac agatgctcga tcgcgcgcgg gcgcgcgagg cgcaggcgat cgtggtgctc
     1081 ggcgccggca agcgcacgca cgcgcccgag ttcggcggcg agacggtcaa ccggctcgcg
     1141 cttgagcgcc tgcgctacgc tgccgcgctc gcacgcgaga cccggctgcc ggtgctggtg
     1201 agcggcggcg caccggccgg cgacaccccc gaggcctacc tgatggaggc cttgctgcgc
     1261 gacgaatacg gcgtgccgcc gcgctgggtg gaatcggcct cgcgcgacac gcgcgagaac
     1321 gcacgtttct cggcggttca tctgcaggct gcgggggtga agcggatcct gctggtgacc
     1381 cacgcgatgc acatggagcg cgcgcgcccc gagttcgagg ccgccggact cgacgtgatc
     1441 ccggcgccga cggcctggct gggcggcggc gacagccccg gcgacgccca gcccctgcct
     1501 ttcgcgccaa gccagaacac cgcctacgcg gcctggttcg cgctgcacga gctgctgggc
     1561 cggctcgcct accgctggtc gcgctgagac ctgcggagag gtcggaacac gacgaccgcc
     1621 ccgtcgtacc gcagggcgcc ggcagaccgc ctcgccgttc atgaaaaaac ccgcgcccgt
     1681 ctctgaaggc ggatgcgcgg gcgagtgcgg tcgcggatgc ggcagccttc agccgccgaa
     1741 gtcgtcgagc atgatgttct cgcgctcgac accgaggtcg agcagcagct tgatcaccgc
     1801 ggcgttcatc atcgggggac cgcacatgta gaactcgcag tcttcggggg ccgggtggtc
     1861 cttgaggtag ttctcgtaca gcacgttgtg gatgaagccg gtgtagccgg tccagttatc
     1921 ctcgggcagc gggtcggaga gcgcgaggtg ccacttgaag ttggggttct cggcagcgag
     1981 cttgtcgaac tcctcgacgt agaaggcctc gcgcatcgag cgcgcgccgt accagaagct
     2041 gatcttgcgc ttgctgtgca gacgcttgag ctggtcaaag atgtgcgagc gcatcggcgc
     2101 catgcccgcg ccgccgccga cgaagaccat ctcgtggtcg gtgtcgcgcg cgaaaaactc
     2161 gccgaaggga ccgtacaccg tgaccttgtc gcccttcttg aggttgaaca cccaggacga
     2221 catcttgccg ggcgggacat cgtcgcgccc cggcgggggc gaagccacgc ggatgttgaa
     2281 cttcaccaca cccttttcgt cggggtagtt cgccatcgag taggcgcgga tcgtggtctc
     2341 gtcgaccttg gagacgaagc gccacaggtt gaacttgtcc cagtcgccgc ggtactcgtc
     2401 ctggatgtcg aagtccttgt aattgaccac gtggggcggg cactcgagct gcacgtagcc
     2461 gccggcgcgg aagtgcacgt cctccccttc gggcaggcgc agggtgagct ccttgatgaa
     2521 ggtggcgacg ttggggttgg actcgacggt gcactcccac ttcttgacgc cgaagacctc
     2581 ttccgggacc tcgatcttca tgtcctgctt gaccggggtc tggcaggaca gacgccagcc
     2641 ttccttggcg tcgcgcttgg tgaagtggga ctcctccgtg ggcagcatcg agccgccgcc
     2701 ttccatgacc acgcacttgc actgcgcgca ggtgccgccg ccgccgcagg ccgagggcag
     2761 gaagagaccg ttgccggcga gcgtctgcag cagcttgccg ccggcgggga cgacgatctt
     2821 cttctcgccg ttgatgtcga tggtgacgtc gcccgacgaa accagcttgg agcgggcagc
     2881 gatgatgaac acggccagtg cgagcacgat ggcggtgaac atcccgatgc cgaggatgat
     2941 ttcggtattc atcgttgcac ggtctcctta gagctgcacg cccgagaacg acatgaagcc
     3001 gagcgacatc aggccgatgg tgatgaaggt gatgcccagg ccctgcaggc cctcgggcac
     3061 gtcgctgtac ttgagcttct cgcggatgcc ggcgagcagc gcgatcgcca gcgcccagga
     3121 tgcgcccgag cccacgccat acaccgcgct ttcagcaagc gtgtagtcgc gttcgaccat
     3181 gaacagcgag ccggccatga tcacgcagtt gaccgtgatc agcggcagga agatgccgag
     3241 cgcgttgtag aggctgggca tgtacttgtc gagcagcatc tcgaggatct gcacgatcgc
     3301 cgcgatcacg ccgatgtagg acagcaggcc gaggaaggac aggtcgacct ccggcagccc
     3361 cgcccacgcc agcgcgcctt cgcgcagcag caggttgtac atcaggttgt tggccggcac
     3421 ggtgatcgcc tgcacgacga cgaccgccac gcccaggccg atcgcggtct cgaccttctt
     3481 ggagatcgcg atgaacgagc acatgccgag gaagaaggac agcgccatgt tctcgatgaa
     3541 cacggagcgc acgaacaggc tgatgaggtg ttccatttca gtaggcctcc ttttccacga
     3601 cctgcggcgc catccggaag gcaggcttct cgacctgctc cttcttccag gtgcgcaggc
     3661 cccagatgat caggccgatc aggaagaagg ccgagggcgg cagcagcagc agcccgttgg
     3721 gcacgtacca gccgccgtcc ttggcgagcg cgatgatctc gatgccgaac agcttgccgg
     3781 caccgaagag ctcgcggatc acgccgagcg tgatcagcat cgcgctgtag ccgagaccgt
     3841 tgccgatgcc gtccatgaag gacagcagcg gcgggttctg catcgcgaag gcttccgcac
     3901 ggcccatcac gatgcagttg gtgatgatca ggccgacgaa caccgaaagc tgcttggaca
     3961 ggctgaaggc gtaggcgcgc aggagctggt ccaccacgat gaccagcgag gcgatgatca
     4021 ccatctgcac gatcatgcgg atcgagctcg ggatctggct gcggatcatc gagatgaaca
     4081 tgctcgagca cgccgtcacc agggtcagcg cgatcgacat cacgaccgcg gtcttgaggt
     4141 tggaggtgac tgcgagcgcc gagcagatgc cgaggatctg cagcgcgatc gggttgttgt
     4201 tgaggatggg gttgaggagg acttccttga cggtgggctt ggacatgatc agaccccctg
     4261 ggtgcggagt ttggcgagat aggggccgaa gccctgttcg ccgagccaga actggagcag
     4321 ccggtcgacg ccgttgctgg tcagcgtggc accggcgagg ccgtcgacct ggtgcttcgc
     4381 gtcggggctg gccggatcga ccccgccctt gacgatgtgg atcgcgggct tgccttgcgc
     4441 gtcgaacagc accttgccgg gccacagcgc cttccacttc gggttgtcga cctcgccgcc
     4501 gaggcccggt gtctcggcgt gctggtagaa gccgaggccg accacggtgt tgaggtcgcc
     4561 cttcaaggcg acgaagccat gcagcgtgga ccacaggccg tagccgcgca ccggcaggat
     4621 cagcgattcg agcttgccgt ccttctccac catgtagacc gtggtgaagc gctcgcggcg
     4681 cttgatcagc gcgatgtctt cctcgccgtc gagggcgtcg gagagcttgg gatcgcgcgc
     4741 agccttgagc gggtcgaaaa gctgggcatc gtgggcgtcg gtgtattcgc cggtctgcag
     4801 atcgaccacg cgcgccttga tgcgcccggc gaagagatcc ttgacctcgc gcgaagacat
     4861 ctcggcatcg cccaggccgg caatcgaaag gatgctgcgc tgcttgtcga gcaggcggtt
     4921 ttccacctgc accggcttca gcgacaccgc cgcgccggcg acgaagaccg agctcaccag
     4981 actgaccgcg agcgccacca tcagcgttcg gcttgtggat tctctattgg acattgcgcg
     5041 caagcctccg cttgatgttg gccgtgacca cgaagtggtc gatcagcggc gcacaaaggt
     5101 tgccgaacag gatggcgagc atgatgcctt cggggaaggc ggggttcacc acgcgcacca
     5161 gcacggtcat gatccccacc accgcaccga agatccattt gccggtgttg gtcatcgagg
     5221 ccgacaccgg atcggtggcc atgaacatca tgccgaaggc gaagccgccc atcaccaggt
     5281 gccagtgcca gggcacggcg aacatcgggt tggtgtcgga gccgatcgcg ttgaacagca
     5341 ggctggtggc gaccatgccc agcatgacgc cggagacgat gcgccacgag gcgatcttca
     5401 tcaccagcag caccgcaccg ccgatcagga tggcgagcgt gctggtctcg ccgatcgagc
     5461 cgtgcaccgt accgaggaaa gcgcgcatcc agtcgatgcc cgcgccggtc accgcctcga
     5521 tgccgccggc ggcgccgagc gacaacatgg tggcgccggt gaagccgtcg accgcggtcc
     5581 acaccgcgtc gcccgacatc tgcgccgggt aggcgaaata caggaaggcg cggccggcga
     5641 gcgccgggtt gaggaagttc ttgccggtgc cgccaaagac ttccttcgcg atcaccacac
     5701 cgaagctgat gcccagcgcc acctgccaga gcggggtgtc gggcggcagg gtgagcgcga
     5761 acagcaccga ggtgacgaag aagccctcgt tgacctcatg cttgcggatc gaggcgaaca
     5821 gcacctccca gaagccgccg acgatgaagg tcgtcaggta gatcggcagg aagtaggtcg
     5881 ccccgtgcag gaagttggcc cacaggctgg cgggatcgag cccggccagc gcgctggtga
     5941 gcgcgatgcg ccagccttcc tgcgcggcca tcagctcggg gctgccggcg aggatggtgt
     6001 tggcctggta gccgacgttc cacatgccga agaacatggc cgggaaggtg cacagccaca
     6061 ccgtgatcat catgcgcttg aggtcgagac cgtcgcgcac gtgggcggtg ttgcgggtga
     6121 ccaggggcgg cttgtagagc gcggtgtcca cggcctcgta caaggcgtag tacttctcgt
     6181 acttgccgcc cttctcgaaa tgatgctcga tgctgtcgag ccaggagcgc agactcatgc
     6241 tcaaccctcc ttctcgatgc gggtcaggtt gtcccgcagg atgggaccgt attcgtactt
     6301 gcccgcgcac acgtaggaac acagcgcgag gtcttcctcg tccagctcga gacagccgag
     6361 cttctgcgcc atgtcggtgt cgcccacgat gagcgcgcgc agcaattgcg tgggcaggat
     6421 gtcgagcggc atcacctctt cgtagttccc gatcggcacc atcgcgcgcg gcgagccgtt
     6481 ggtcgaggtg gtgaagtcga acagcctgcc cggcgagagc ttggagatgt agaggttcat
     6541 caccgagtgc ttgttcgccc cggcgcgcag gtagtgcagc atctcgcgct cggagccttc
     6601 ttccaggcag gacacctgca ggtggtagcg cccgaggaag gcgcaggctc cgcgcgaggt
     6661 ccgccccccg aacaccgagc cggagatcac gcgcaccttc ttgccggtct tcagttcgcc
     6721 ggcgacgagc tcgtcgaggt tggcgccgac gcgggtgcgc acgaggcgcg gcttcgcggc
     6781 catggggccg gcgatcgaca ccacgcgctc ggtccacagg cggccggtgg agaagagctt
     6841 gccgatcgcg atcgcgtcct ggtagttgag cgaccacacc gacttctcgg catgcaccgg
     6901 atcgaggaaa tggatgtgcg tgccggcgag gccggccgga tgcgggccgg cgaaggtctc
     6961 ggtgcgcacg tcggccagcc cggaacaggg catccgggtc ccatcggcgt ggcacacgac
     7021 gaccggcgcg atgcgcgcga gcacgcgcac gccgcgagcg aagtcgtccg tgtgctcggc
     7081 gatcactacg gtcgggtcgg cggcgagcgg atgggtgtcg atcgcggtga cgaagatcga
     7141 gtgcggcttc gcgtcgatcg ccggcacctt gctgaagggc cgcgtgcgta gtgcggtcca
     7201 cagtccagag tcgatcagct tgctgcgcac cgtcgcgtcg tcgagcctgt cgagttcggc
     7261 gtccgtgtag cgcgtgaatt cgaccgcatc gtcgccgtcg aggtcgatca ccaccgattg
     7321 cagcacgcgc tgctcgccgc gatggatggc gctgacggtg ccggcacccg gcgcggtgaa
     7381 gaccacaccg ggcgttttct tgtcggagaa caggacctgg ccgagcttca cccggtcacc
     7441 gacctgcacc gccatggtcg gtttcatgcc gtgatagtcg aagccgacga cggccacgct
     7501 gcgcaccggc ctgccggcct cgatgcgctg ctcgggcgcg ccggcgatgg gcaggtccag
     7561 accgcgtttg attctgatca tgcgcttctc gcccgttgat tccactggag gcacgggcgc
     7621 cgtcaacgca tccgggagcg ccaggcgtcc ggacgccgga ggcggtcccg cgtcgataaa
     7681 cgcgcggatt ataaaagatt gttaccagac gatcacgccg gggaataccc gaaggcagcg
     7741 aactaggtga aatccacaat acgccgagcg cgccatcaac cggccaagaa attccgttcg
     7801 catgagaatg ctgcaaatgg gatgcgctcc cgaagcacgc gaggcgccct ccaaaacgac
     7861 agctcacgct cagcatggta gaagctcggc gcaagggaag tcggggatgg acttcccgtg
     7921 cgccggtcac gctcacacgc gctcgaacac ccccgccgca cccatgccgg tgccgatgca
     7981 catgctgatc atgccgtagc gcaggccggg gtcgcgctgc atcgccgcca tcagcgtggc
     8041 ggtgcggatc gcgccggtcg cgcccagcgg gtggccgagg gcgatcgccc cgccgagcgg
     8101 gttgaccttc gccggatcca tcccgagctg gcgcatcacc gccagcgcct gcgcggcaaa
     8161 ggcctcgttg agctcggtcc agccgatgtc ggccagcgcc acgccggcac gcgcaagcgc
     8221 acgcgggatg gcctcgacgg ggccgatccc catgacctcg ggcggcacgc cggcgaccgc
     8281 gaagctggaa aagcgcgcga tcggggtcac gtcgtagcgc tttaacgcgg tctcgctcat
     8341 cagcagcacc gccgcggcgc cgtcggacat ctgcgagctg ttgcccgcgg tcaccgagcc
     8401 gcgcgccgcg aacaccggct tgagcttgcc gagcctgtcg gcagcggcgt ccggacgcgg
     8461 gccctcgtcg tgctcgacca ggcgctcggt gatgcgcacc gtgccgtcga cacccggggc
     8521 ataggacttc accgcatagg gcaggatctc gtccgcgaac tggccggcgg cgatcgcggc
     8581 acaggcgcgc cggtgcgact cgaccgcgaa cgcatcctgg tcctcgcggc tcaccttcca
     8641 ttgctgtgcg accttctcgg cggtgaggcc catgccgtag gcgatggaga ggttctcggc
     8701 gcgcgcgaag atctcggggt tgaggctgac cttgttgccc atgatctgcg gcatcgcgct
     8761 catgctctcg gtgccggcgg cgatcatcac gtccgcctcg ccgaggcgga tgcggctggc
     8821 ggcatcggcc acggcctgca ggccggaggc gcagaagcgg ttgatggtga tgcccggcac
     8881 gctgtcgggc agcccggcca gcagcaggcc gatgcgtgcc acgttcatgc cctgctcggc
     8941 ctcgggcatc gcgcagccgg tgacgacgtc gccgatctcg cgcgcgtcga gattcggcac
     9001 cttggcgacc acctcgcgca gcaccctggc cagcaggtcg tcggggcgca cggcgcggaa
     9061 ggcaccgttg cgcttggcga ccggcgtgcg caccgccgcg acgatgtagg cgtcctggat
     9121 ctgtttgctc attgtctgtc tctccctcag ttgcgcaggg gcttgccggt ttccagcatg
     9181 tgcgcgatgc gctgctgggt cttctcggtc ttgagcagtt cgacgaactg cgcgcgttcg
     9241 acctcgagga tccactgctc gctcacgcgg cggttggtgt cgacctcgcc accgcacagc
     9301 gcggtggctg cggccttggc gacgcggtaa tcgtgcgccg agatgaaacc gccctcggcc
     9361 atgttcacca gcatcatttc gcaggtcgcg atgccgttgc gtccggccac ggtgaccgcc
     9421 ggattgacca gcgccggccg ccaggccgac tcggccagcg cgcgcgcgcg gcggatcgcg
     9481 gcgtagagga tctcgttggc gttgaagagg atgtcgtcgc cggtgcggcc gaagccgagc
     9541 tgcaccgcct cctgcgcgct cttcgccacg gtggccatgg cgatggtctg gaaggcgttc
     9601 tggatgtaag ggaagacgtc gcccccggcc gcgcgctgcg ccagcgcatg cgcctgcagg
     9661 gcgaactcct tgctgccgcc gcccgccggg atcaggccca cgcccgcctc caccaggccg
     9721 acgtagctct cgagcgcgaa cacccgatgc gccgcgtgca tcacgaactc gcagccccca
     9781 cccagcgcca tgccctgcac cgcggccacg acgggaacct gcgcgtgctt gatcgccatc
     9841 gaggtgcgct ggaacttggc caccatcctt tccagcagct cgaactgccc ggcacggcag
     9901 gcctcgccca cctgctgcag gttggcgccg accgcgaagg gcgccggctg ccagatcacc
     9961 aggccgtcga gatccgtctc ggcgcgcgcg atggcctcga ggaggccatc catgacctcc
    10021 tcgcccaccg cgtgcatctt cgaggtgatc gacacgatgc cgatgcgcgc gtcctggtcg
    10081 gcgcgccgcc acaggcgtac gccggcgttc tcccacagtg tctcgccgcg atcgtccggc
    10141 gcctcgccga gcacgcggtc ggggtagagc tggcgctggt acaccggcag cgcggagcgc
    10201 ggcttgagcg agtcctcggc cgcgctgtag gagccggccg gcgcatgcac gccgtcgcgc
    10261 tcgaacaccc aggccggcag cggcaccgcg gacatggcgc ggccatgcgc gatgtcgtcg
    10321 cggatcattt cggcgactgc cctccagccc gcagcctgcc aggtctcgaa cggcccctgc
    10381 gcccagccga agccccagcg catcgcgagg tcgatgtcgc gcgcgctgtc tgcgatgtcg
    10441 cccagatgca ccgcgcagta gtggaacacg tcgcgaaaga tcgaccacag gaactgcgcc
    10501 tgcggatgag ggtgcgcggc gagctgggcg aacttctccg ccgggttctt gagcttgagg
    10561 atctcggcca cctcggggag cacctcgccg ccgctctcgc ggtagtcctg cagatgcagg
    10621 tccagcacct ggatctgcct gccctgcttg cggtagatgc cgcatttggt cttctggccg
    10681 agcgcgccct tggcgatcag cgcctgcagc cactcgggcg tcctgaagtg cgcgcaccag
    10741 gggtcgtcgg gcagcgtcgc ctgcatggtg ccgagcacat gcaccagggt gtcgaggcca
    10801 accacgtcgg cggtgcggaa ggtggcgctc ttcggacggc cgatgcgcgg gccggtgagc
    10861 aggtccacct cgtcgaaggc cagcccgagg cgggcggtgt ggtgcagcac ggcgaggatg
    10921 gagaacacgc cgacgcggtt ggcgacgaag ttgggcgtgt ccttggcacg cacgatgctc
    10981 ttgcccaggt gcgtggtcag ccaggcctcg aggccgtcaa gcagcgccgc gtcggtggcg
    11041 gcaatgggga tcagctccac cagcggcatg tagcgcggcg ggttgaagaa gtggatgccg
    11101 cagaactggc tgcggcgatc ggccggcatg cccgtggcga gcgcctcgat cgacagtccg
    11161 gaggtgttgg aggcgaagat cgcccccgcc ttcaggtgcg gcgcgacctt ggcgtagagg
    11221 tcgagcttcc actccatctt ctcggcgatc gcctcgatga caaggtcgca ttcgcccagg
    11281 cgcgcgaggt cgctgccgta gttggcggac tcgacgtact gcgcgcggtc cttcgcggcg
    11341 aagggcgcgg ggtcgagctt cttcatgccg gcgagcgcct tgtcgacgac gccgttggcc
    11401 gggccttcct tggccggcag gtcgaacagg atcaccggca cgtcggcgtt ggcgcaatgg
    11461 gcggcgatct gcgcccccat gacgccggcg ccgagcacgg cgactttgcg gatgatgaga
    11521 cggctcatgc ggttctccgg atgacaggaa atgcaggacg caagagacac ggcccgcgcc
    11581 gggagcgacg cgggccgtga gcgcggcgtc gatcagaacg acatcgagta ctgcgcgccg
    11641 aggatccaga cgctggaatc gtattcgccc ttgacgaggc ccttgctcag atcggccgcg
    11701 ttgccgttgt tgattttcgt gtcgtctacg tacaggtagg acacgcccat gtcgagagcg
    11761 gaagacttgc tcggcttcca ctgcccaccg aacgagaacc agatgcggtc gttgtcgggc
    11821 agcgaggtca ggcgcttctc cgggttcttc accggtgtct cgtcgaaggc caccccgaac
    11881 ttcagcttcc atgcgtcgtt gagctggtag ttcgcgccga gggcgacgcg ccaagtgtcc
    11941 tggaagtcgg tatccagggt ctgcgccttc tcgagcgggg taccaagcaa gagcccggat
    12001 gtccgcatga tgtcaacttt gtcgacactg ctccagccag tccaggacac atcccccagc
    12061 agttcccagc gggaatcaag cttgtgcgac aagctcagga tgaacgtatc cggaagttcc
    12121 acgtcggcag atgcgctcac accggtagtc aacggatgca ggtttgcagc cgtaccactg
    12181 aactgcaagg accccttcaa ttcatgc
//
LOCUS       DAOQNN010000048        12204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2303_length_12204_cov_10.352045, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000048 DAOQNN010000000
VERSION     DAOQNN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12204
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2303_length_12204_cov_10.352045"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..1558
                     /locus_tag="PKV97_04765"
     CDS             383..1558
                     /locus_tag="PKV97_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB05222.1"
                     /translation="MRAPPLLVIVLAQLFGTSLWFSANAAGEDLLRTWALQPADIGRL
                     TIAVQLGFIAGTLGFSLSGLADRFPASRIFAVCAVIGAVANAGFALLAHGLGEGLAWR
                     FTVGLALAGVYPLGMKLVVSWVPQQAGAALAWLVGMLTLGTALPHGVRFLDAGWGWQA
                     TLLVSSLLALLAAAMIFTLGDGPHLARRAGAPRLRLGEGLYALRAPDFRASALGYFGH
                     QWELYAFWTLVPLLVSTACAAEAGSPSGPAFAVIGIGAVGCVLGGWLSRHIGSARVAA
                     LALAVSALCCALYPFATAWPPLALAGLMLLWGASVVADSPHFSALSARACPPESVGSA
                     LAFQNAIGFAITMVSIELVTRLWPSIGDRVAWLLLPGPVLGLLALRPLWSRSHVEAR"
     gene            1653..2057
                     /locus_tag="PKV97_04770"
     CDS             1653..2057
                     /locus_tag="PKV97_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05223.1"
                     /translation="MMKPCKRVFAALALLALAAPGWTQAVVEDAPMLRLECGGIGLEE
                     SERMRAEIGMHALTVFFSTTAGGWVTDVETRVDEPLSGQRVEASCGPIGQVDVPAPGR
                     YRISASSAGARQEHWVDLVPQGGARLSLRWQE"
     gene            2163..>3376
                     /locus_tag="PKV97_04775"
     CDS             2163..>3376
                     /locus_tag="PKV97_04775"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNB05224.1"
                     /translation="MSVQRVLTGITTSGTPHLGNYAGAIRPAVAASRQPGVESFYFLA
                     DYHALIKTTDPLRVQRSTLEIAATWIAAGLDTDRVWFYRQSDIPEIPELTWLLTCVCG
                     KGLLNRAHAYKAAVDKNTADGVDPDAGVNMGLYMYPVLMAADILMFKAHSVPVGRDQI
                     QHIEMARDMAGSFNHLYGEHFVLPEAAIDASVATLPGLDGRKMSKSYDNTIPLFAPTA
                     ELKKLIFSIVTDSTAPGEPKDADGSALFQLYQAFSTAEETRAMRTAFDEGIAWGEAKQ
                     ALFEKIEAAVAPMREHYLALIAEPARIEKHLHEGAAKARAIATPFLGELRRAVGLRNL
                     ASVAGEAKAEKAKTAQPQFKQYREADGRFHFKLIDADGRLLLQGEGFASPREAGAVVA
                     ALKAGSAFEMKEA"
     assembly_gap    3377..3476
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3669..4598)
                     /locus_tag="PKV97_04780"
     CDS             complement(3669..4598)
                     /locus_tag="PKV97_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HNB05225.1"
                     /translation="MNFQQLRIIRETVRRGFNLTEVANALFTSQSGVSKHIKDLEDEL
                     GIELFVRKGKRLLGLTPPGEELVVMVERMLLDAANIKRLADQYRGRDEGQLTVVTTHT
                     QARYALPKVVAAFKRAYPRVLLKLHQGSPHEIVQMLLDGEADIGVATEALAEVPELAS
                     FPYYGWQHAVVVPAGHPLEEAHPLTLEAIATHPLITYHEGFTGRTRIDQTFARAGLTP
                     DVVMSALDADVIKTYVELGLGVGILASMAFNPARDPGLRKLDSDHLFAENVTRIAVRR
                     GHYLRGFAYRFIELCAPELTETVVADAVGGTAG"
     gene            complement(4595..5692)
                     /locus_tag="PKV97_04785"
     CDS             complement(4595..5692)
                     /locus_tag="PKV97_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNB05226.1"
                     /translation="MSIQVRNIHKRFGSFVALDDVSLDFPTGELVALLGPSGCGKTTL
                     LRVIAGLEQADAGQVLLDGEDASGTHVRDRQVGFVFQHYALFRHMSVFDNVAFGLRMK
                     PRGQRPTEKQIAEKVHALLDLVQLDWLADRFPAQLSGGQRQRIALARALAVEPRVLLL
                     DEPFGALDAKVRKELRRWLRKLHDELHVTSIFVTHDQEEALEVADRVVLMNQGAVEQI
                     GTPEEVYRRPATPFVYGFLGAVNLFHGRVDGEGLRVGDARLPQQARGFGEGAEVLAFA
                     RPHELDIVPDAGAGEGIAARVSRVLAFGITARVELDGLNGAQGQHFEVEITRERVDRL
                     GLAEGQAVRLLPSHLSLFESRQAATTAGAAR"
     gene            complement(5689..6597)
                     /gene="cysW"
                     /locus_tag="PKV97_04790"
     CDS             complement(5689..6597)
                     /gene="cysW"
                     /locus_tag="PKV97_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNB05227.1"
                     /translation="MAGATTLRLGAAQAIGQTRFEARAATRETALVKWLILGTALTFF
                     AVFLLMPLIAVFVEALRKGWETYASALVDPDALSALRLTLLAAAIALPLNLVFGVCAA
                     WAIAKFEFRGKHFLITLIDLPFSVSPVIAGLIYVLVFGAQGWFGPWLAEHDVKIIFAV
                     PGIVLATVFVTFPFIARELIPLMQAQGKEEEEAAVVLGANGLQTFWHVTLPNVKWGLL
                     YGVILTNARAMGEFGAVSVVSGHIRGETNTLPLHVEILYNEYQFAAAFAVASLLALLA
                     LVTLGIKTWIEHRAAHTQHDIHEGQP"
     gene            complement(6599..7435)
                     /gene="cysT"
                     /locus_tag="PKV97_04795"
     CDS             complement(6599..7435)
                     /gene="cysT"
                     /locus_tag="PKV97_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNB05228.1"
                     /translation="MSTAKTFRVLPGFGPTLGYTLAYLSLLVLIPLAAVFLKTAELTF
                     AEFWNVVTAPRVVASYKLSFGMSLLAAAINAVFGLMLAWALVRYSFPGKKLIDALVDL
                     PFALPTAVAGIALTALYAKNGWIGQFLEPLGIQVAFKPLGVLVALVFIGLPFVVRTVQ
                     PILEDLDTELEEAATSLGAQRWQAFRHVILPIVFPALLTGFALAFARAVGEYGSVIFI
                     AGNLPMVSEITPLMIITKLEQYDYTGATAIATVMLILSFALLLLINGLQAWTAKRTGR
                     DR"
     assembly_gap    7662..7761
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7778..8035
                     /locus_tag="PKV97_04800"
     CDS             7778..8035
                     /locus_tag="PKV97_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05229.1"
                     /translation="MTDFFGNFLLALLLVGLIRFLYAVCALLVRTRDGGNIAFAIALP
                     VALLYIAVRNPVPVGSWAGLGAGTVLGAVLLLAFARWRRRS"
     gene            8154..8474
                     /locus_tag="PKV97_04805"
     CDS             8154..8474
                     /locus_tag="PKV97_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB05230.1"
                     /translation="MPIATRALAALAAAAALALPFSVQAAPDDRPVRVWAAACAACHG
                     TDGHAQAGMTSLAGRNADELYTLLIEFKRDVRPATVMHQHAKGYTDEELQRLAQWFAA
                     QAKR"
     gene            8485..9768
                     /locus_tag="PKV97_04810"
     CDS             8485..9768
                     /locus_tag="PKV97_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB05231.1"
                     /translation="MSTIDRRGFIRSAGGLALALGAGVGVGACATPVGRAAPGRVVVI
                     GGGFGGATAAKYLKKWEPRLEVTLVERNQNFVSCPISNLVLAGVKRIEDITLAYDGLA
                     RYGVRLVRDEAVAIDVDKREVRLAKGDPLPWDRLVVAPGIDFLYEQIPGLNNADARQQ
                     VLHAWKAGAQTVALRKRLEELPDGGVFAISIPKAPYRCPPGPYERASVVAHYFKQHKP
                     RSKVLILDGNPEITSKKALFLQAWEERYKGIIEYRPDSVLQDFDVRANVGKFEFESVR
                     AELFNVIPPQRSGLIASPFANVGGRWAGVDFRSFESTAVPGVHLIGDAIATAPGMPKS
                     GFMANNHAKVAADAVIASLTGLEPNPTPIIANTCYSFVSDRDVVHVASIHKFDTQENT
                     LLPVKGAGGLSAVANDLEGKYALAWARNIWADALT"
     gene            9810..10658
                     /locus_tag="PKV97_04815"
     CDS             9810..10658
                     /locus_tag="PKV97_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB05232.1"
                     /translation="MDQDPGGPSRALAERTRVFLDRLPLARDGGRQLRRLPEGGVVGD
                     WFGCELSSVFQPIVIPGTGAITGYEAYLRILGSGERSLSPWTLFSANADDDRLIALDR
                     LARTAHALNFVTSVDRDSLLFLNVHGRLLAAVSGDHGAAFRKVVDALGLAPERIVIET
                     PLAASSQPELLAFVLRNYRNNGFQVAINVDSPMQWQALSSLVPAQFVKIDSARLFIAD
                     DWRARLEWLATLRERADIVVTRLERKLDGALPEGVHLQGHAYGLPAQRAAIMRAERHV
                     LDRFAF"
     gene            10677..12074
                     /locus_tag="PKV97_04820"
     CDS             10677..12074
                     /locus_tag="PKV97_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNB05233.1"
                     /translation="MAASCPQEIFIIGGGFSGTLTAVHLLREARPGQLRVTLIEQHRR
                     AARGLAYRIWDDSMLLNVPAGNMSALADEPGHFLAYCRDIDPAFNEGSFVPRRLFGDY
                     LEHTLQVAERGSRTPFTRIRAQAVAVRRKQASPLLQIELDDGVTRQADQVVLALGHLG
                     AQPLHFADALAGSSRYIGNPWDYAAMDQIPAGGPVVVLGTGHTAIDALFRLTSRDDER
                     KVFLLSRRGLLPKGHRTLPQPPPTTTFPGYLEDLPPTTFAYLRAIRRQVRLRQRQGCN
                     WRDVVNALRPHTPEIWRRLPEMERRRFLSRVGPWWDIHRHRLAPTAYRRLRGMLASGQ
                     AAVVAGQVLQMHEHGPGVRVIVRPRGGSEAREWDVAAVVNCTGPDYDIDRLTASPLVA
                     QLRDEGCVRADPLHIGIDVDAGYHVVGADGLKVSGLHYVGPMLRARYWEAIAVPELRR
                     HAHNLARHLLTPGQA"
BASE COUNT         1773 a         4086 c         4244 g         1901 t
ORIGIN      
        1 cgaacaccgc gccgccttcg gggagggcga acatggtggc gagcaggcgg cccacgaggc
       61 cttgccaggg cgcctcggcg tccgggctgg tggggtgggg gcgcaggccc acggtgatga
      121 cggggacgcg gggacgttcg gtaagaggct gggtcacggt gtgggctgac aataagggaa
      181 ctggcaacga ggggccggcc gggaggctgg tctgcgcggg ccgcgcacag ccgcggtgtg
      241 catgcggccg ggaagcgcaa tgttaacccg ttcgggcagc cgcgcccacg gggccgccgc
      301 ggcacggcct ggcgggcgcg ccgtcctgcg cgtggatcgc cgtgcttgag cccccgcgag
      361 ccctgtcgca cactgctgcg ccatgcgcgc ccctccactc ctcgtcatcg tcctcgccca
      421 gctcttcggg acctcgctgt ggttttccgc gaacgccgcc ggcgaggacc tcctgcgcac
      481 ctgggcgctg cagccggccg acatcggcag gctgacgatc gcggtgcagc tcggcttcat
      541 cgccggcacc ctgggattct cgctcagtgg cctggccgac cgctttccgg cgagccgtat
      601 cttcgccgtc tgcgcggtca tcggggcggt ggccaacgcc ggcttcgcgc tgctcgcaca
      661 cgggctgggc gagggcctgg cgtggcgctt cacggtgggg ctcgcgctcg ccggcgtgta
      721 tccgctcggg atgaagctgg tggtgagctg ggtgccccag caggcgggcg cggcgctggc
      781 ctggctggtc ggcatgctga cgctgggcac ggcgctgccg cacggggtgc gctttctcga
      841 tgccggctgg ggctggcagg ccacgctgct ggtgtcctcg ctgctcgcgc tgctggcggc
      901 ggcgatgatc ttcacgctcg gcgacggccc gcacctcgcc cgtcgcgccg gtgcgccgcg
      961 gttgcgcctg ggcgaggggc tgtacgcgct gcgtgcgccg gacttccgtg cctccgccct
     1021 cggctatttc gggcatcagt gggaactgta cgccttctgg acgctggtgc cgctgctggt
     1081 gagcacggcg tgcgcggccg aggcaggttc gccgtccggg ccggccttcg cggtgatcgg
     1141 catcggtgcc gtcggctgcg tgctcggcgg ctggctcagc cgccacatcg gcagcgcgcg
     1201 ggtggctgcc ctggcgctgg cggtgtcggc gctgtgctgt gcgctgtatc ccttcgccac
     1261 cgcctggccg ccgctggcgc tggccgggct gatgctgctg tggggcgcga gcgtggtcgc
     1321 cgactcgccg cacttctccg cgctgtcggc gcgcgcctgc ccgcccgaga gcgtgggcag
     1381 cgcgctcgcc ttccagaacg cgatcggctt cgcgatcacc atggtgtcga tcgaactcgt
     1441 cacccggctg tggccctcga tcggcgaccg cgtcgcctgg ctgctgctgc cggggccggt
     1501 gctcggcctg ctcgcgctgc ggcccttgtg gtcgcgcagc catgtcgagg cgcgctgagc
     1561 ggagcggacc ttttccggct gtgcttgccg gcgcgcgggg agccgggaac aatgcgggtg
     1621 cccgatccgg gcggtaccgg gagtcgcacc agatgatgaa accctgcaaa cgcgtgttcg
     1681 ccgcccttgc cctgctcgcg ctcgccgctc ccggatggac gcaggcggtg gtcgaggatg
     1741 cgcccatgct ccggctcgag tgcggcggca tcgggctcga ggagtccgag cgcatgcgcg
     1801 ccgagatcgg catgcatgcg ctgaccgtgt tcttctccac caccgcgggc ggctgggtca
     1861 ccgacgtcga gacgcgggtc gacgagccgc tctccgggca gcgcgtcgag gcgtcgtgcg
     1921 ggccgatcgg gcaggtcgac gtgcccgcgc cggggcgcta ccggatctcc gcgtcctccg
     1981 ccggcgcgcg ccaggagcac tgggtcgatc tcgtgccgca gggcggtgcg cgtctctcgc
     2041 tgcgctggca ggagtgagcg cgatcgaccc ggcgcgcacg cccccgtagc gcatccgcct
     2101 tatccgcacg cgtcgaagcc gttaaactcg cgctccctga ccaattcgag ccggagcgat
     2161 ccatgtctgt ccagcgcgtc ctcaccggga tcaccacctc gggcacgccc cacctcggca
     2221 actacgccgg cgccatccgt ccggcggtcg ccgccagccg ccagcccggc gtcgagagct
     2281 tctacttcct ggccgactac cacgcgctga tcaagaccac cgacccgctg cgcgttcagc
     2341 gctcgacgct ggagatcgcc gccacctgga tcgccgccgg cctggacacc gaccgcgtgt
     2401 ggttctatcg ccagtccgac atccccgaga tccccgagct cacctggctg ctcacctgcg
     2461 tgtgcggcaa gggcctgctc aaccgcgccc acgcctacaa ggcggcggtg gacaagaaca
     2521 ccgccgatgg cgtcgatccg gacgccgggg tcaacatggg cctctacatg tacccggtgc
     2581 tgatggccgc cgacatcctg atgttcaagg cgcactcggt gccggtcggg cgcgaccaga
     2641 tccagcacat cgagatggcg cgcgacatgg cgggcagctt caaccacctc tacggcgagc
     2701 acttcgtgct gcccgaggcc gccatcgacg cctcggtcgc caccctgccc gggctggacg
     2761 gccgcaagat gagcaagagt tacgacaaca ccatcccgct gttcgcgccc actgccgagc
     2821 tgaagaagct gatcttctcc atcgtcaccg actccacggc gccgggcgag cccaaggatg
     2881 ccgacggctc ggcgctgttc cagctctacc aggccttctc gaccgccgag gagacgcgcg
     2941 ccatgcgtac cgccttcgac gagggcatcg cctggggcga ggccaagcag gcgctgttcg
     3001 agaagatcga ggccgcggtg gcgccgatgc gcgagcacta cctggcgctg atcgccgagc
     3061 cggcgcgcat cgagaagcat ctgcacgagg gcgccgccaa ggcgcgcgcg atcgccaccc
     3121 ccttcctcgg cgagctgcgc cgcgcggtgg gcctgcgcaa tcttgccagc gtggcgggcg
     3181 aggccaaggc cgagaaggcg aagaccgcac agccccagtt caagcagtac cgcgaggccg
     3241 acggccgctt ccacttcaag ctgatcgacg ccgacggccg cctgctgctg cagggcgagg
     3301 gcttcgcctc gccgcgcgag gcgggcgcgg tggtggcggc gctgaaggcg gggagcgcct
     3361 tcgagatgaa ggaggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggg
     3481 cgcggtggtg gcggcgctga aggcggggag cgccttcgag atgaaggagg cagcgctgtg
     3541 gctggccggc gtgccggtgg cggtgctggc cgacggcgtc gacgaggcca ccctgtgcgc
     3601 ggcgctgggc gccttcgccg actgagcctg cgggcggcag gcgcccgctc ggctggcgac
     3661 gcgccggttc agcccgccgt gccgcccacc gcgtccgcga ccaccgtctc ggtcagctcg
     3721 ggcgcgcaca gctcgatgaa gcggtaggcg aagccgcgca ggtagtggcc gcgacgcacc
     3781 gcgatgcggg tgacgttctc ggcgaacagg tggtcgctgt cgagcttgcg caggcccggg
     3841 tcgcgcgccg ggttgaaggc catcgaggcc aggatgccca cgcccagccc gagctccaca
     3901 taggtcttga tcacgtcggc gtcgagcgcc gacatcacca cgtcgggcgt cagcccggcg
     3961 cgggcgaagg tctggtcgat gcgggtgcgg ccggtgaagc cctcgtggta ggtgatgagc
     4021 ggatgcgtgg cgatcgcctc cagggtcagc gggtgcgctt cttcgagcgg gtgcccggcg
     4081 ggcacgacca ccgcgtgctg ccagccgtag taggggaagg aggccagctc cggcacctcg
     4141 gccagcgcct cggtggccac gccgatgtcg gcctcgccgt cgagcagcat ctgcacgatc
     4201 tcgtgcgggc tgccctggtg cagcttgagc agcacgcgtg ggtaggcgcg tttgaaggcc
     4261 gccaccacct tgggcagcgc gtagcgcgcc tgggtgtggg tggtgaccac cgtgagctgg
     4321 ccttcgtcgc gcccgcggta ctggtcggcc aggcgcttga tgttggcggc gtcgagcagc
     4381 atgcgctcga ccatcaccac cagctcctcg ccgggtgggg tgaggccgag caggcgcttg
     4441 cccttgcgca cgaagagctc gatgccgagc tcgtcctcga ggtccttgat gtgcttggac
     4501 accccggact gcgaggtgaa cagcgcgttg gccacctcgg taagattgaa cccgcgccgt
     4561 acggtctcgc ggatgatgcg caactgctgg aaattcatcg ggcggctccg gccgtcgtgg
     4621 cggcctgtcg cgattcgaac aggctcaggt gcgagggtag caggcgcacg gcctgtcctt
     4681 ccgcgagacc gaggcggtcg acgcgctcgc gcgtgatctc cacctcgaaa tgctggccct
     4741 gggcgccgtt caggccatcg agttcgacgc gcgccgtgat cccgaaggcc aggacgcggc
     4801 tcacccgtgc cgcgatgccc tcgcccgcgc cggcgtccgg cacgatgtcg agttcgtgcg
     4861 ggcgggcgaa ggccagcacc tcggcgccct cgccgaagcc gcgcgcctgc tgtggcaggc
     4921 gcgcgtcgcc gacgcgcagg ccctcgccgt cgacgcggcc gtggaacagg ttcaccgcgc
     4981 cgagaaaacc atatacgaag ggcgtcgccg gtcggcggta gacctcttcc ggcgtgccga
     5041 tctgctcgac cgcgccctgg ttcatcagca ccacgcggtc ggcgacttcg agcgcctctt
     5101 cctggtcgtg ggtgacgaag atcgaggtga cgtgcagctc gtcgtgcagc ttgcgcagcc
     5161 agcgccgcag ctccttgcgc accttggcgt cgagcgcgcc gaagggctcg tcgagcagca
     5221 gcacgcgcgg ctccaccgcg agcgcgcgcg cgagcgcgat gcgctggcgc tggccgccgg
     5281 agagctgggc cgggaagcgg tcggcgagcc agtcgagctg cacgaggtcg agcagcgcgt
     5341 gcaccttctc ggcgatctgc ttctccgtcg gccgctggcc gcgcggcttc atgcgcagcc
     5401 cgaaggcgac gttgtcgaac acgctcatgt ggcggaacag cgcgtagtgc tggaacacga
     5461 agcccacctg gcgatcgcgc acatgcgtgc ccgaggcgtc ttcgccgtcg agcagcacct
     5521 ggccggcatc ggcctgctcc agcccggcga tcacgcgcag cagcgtggtc ttgccgcagc
     5581 ccgaggggcc gagcagcgcc accagctcgc cggtgggaaa gtcgagcgac acgtcgtcga
     5641 gggcgacgaa gctgccgaag cgcttgtgga tgttgcgaac ctggatgctc atggctgtcc
     5701 ttcgtgaatg tcgtgttgcg tgtgcgcggc gcggtgctcg atccaggtct tgatgcccag
     5761 ggtgaccagc gccagcaggg cgagcagcga ggccaccgcg aacgcggcgg cgaactggta
     5821 ctcgttgtag aggatctcga cgtgcagcgg cagcgtgttg gtctcgccgc ggatgtgccc
     5881 ggacaccacc gacaccgcgc cgaactcgcc catcgcgcgt gcgttggtga ggatgacgcc
     5941 gtacagcagg ccccacttca cgttgggcag ggtgacgtgc cagaaggtct gcaggccgtt
     6001 ggcgccgagc accaccgcgg cttcctcttc ctccttgccc tgcgcctgca tcagcgggat
     6061 cagctcgcgg gcgatgaagg ggaaggtcac gaacacggtg gcgagcacga tgcccggcac
     6121 cgcgaagatg atcttcacgt cgtgctccgc cagccacggc ccgaaccagc cctgggcgcc
     6181 gaacaccagc acgtagatca ggccggcgat caccggcgac accgagaagg gcaggtcgat
     6241 gagcgtgatc aggaagtgct tgccgcggaa ctcgaacttg gcgatcgccc acgccgcgca
     6301 cacgccgaac accaggttca gcggcagcgc gatcgccgcg gcgagcaggg tcaggcgcag
     6361 cgcggacagc gcgtccgggt cgaccagcgc gctggcgtag gtctcccagc ccttgcgcag
     6421 cgcctcgacg aagaccgcga tcagcggcat cagcaggaat accgcgaaga aggtgagggc
     6481 ggtgccgagg atcagccact tcaccagcgc cgtctcgcgc gtcgccgcgc gcgcctcgaa
     6541 gcgcgtctga ccgatggcct gggctgcgcc caggcggagt gtggtggcgc cggccatgtc
     6601 agcggtccct cccggttcgt ttggcggtcc acgcctgcag gccgttgatg agcagcagca
     6661 gggcgaagga caggatcagc atcacggtgg cgatcgcggt ggcgccggtg tagtcgtact
     6721 gctcgagctt ggtgatgatc atcagcgggg tgatctccga caccatcggg aggttgccgg
     6781 cgatgaagat caccgagccg tattcaccca ccgcacgcgc gaaggcgagc gcgaagccgg
     6841 tcaggagcgc cgggaacacg atcggcagga tgacgtggcg gaaggcctgc cagcgctgcg
     6901 cgccgaggct ggtggcggct tcttcgagtt cggtgtcgag gtcttccagg atgggctgca
     6961 ccgtgcgcac cacgaagggc aggccgatga agaccagcgc caccagcacg ccgagcggct
     7021 tgaacgcgac ctggatgccg agcggctcga ggaactggcc gatccagccg ttcttcgcgt
     7081 acagcgcggt cagcgcgatg ccggccaccg cggtgggtag cgcgaagggc aggtcgacca
     7141 gggcgtcgat cagcttcttg ccggggaagg agtagcgcac cagcgcccat gccagcatca
     7201 gcccgaacac ggcgttgatc gcggcggcga gcagggacat gccgaaggac agcttgtagg
     7261 aggccaccac ccgcggtgcg gtgacgacgt tccagaactc ggcgaaagtc agctcggccg
     7321 tcttcaggaa caccgccgcg agcgggatca gcaccagcag cgacaggtag gcgagggtgt
     7381 agccgagcgt gggcccgaag ccgggcagta cgcggaaggt cttggcggtg gacatcagcc
     7441 ttgctccacg atcggctcga agcggtgggc gtggcgggcc tcgcgaccgt ctcggcgatt
     7501 ggcccggcgc gtggcgctgg cgcggcagcg gacgggtatc gggcgggctt tgcgagccat
     7561 gtcggactcc tttcaaaggg gatgaaagcg agtctagtca gttgctgcag ggcaaaaaaa
     7621 taataaaaac cgcgttgctt attcgctttt ctgctaatcg annnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn ngatcaagga gcacgtagtg acggacttct tcggcaattt
     7801 cctgctggcg ctgctgctgg tcgggctgat tcgctttctc tacgccgtct gcgcgctgct
     7861 cgtgcgcacc cgcgatggtg gaaacatcgc cttcgcgatc gccttgcccg tcgcgctgct
     7921 ctacatcgcg gtgcgcaatc ccgtgccggt cggcagctgg gcggggctgg gggccggcac
     7981 ggtcctcggt gcggtgctgc tgctggcgtt cgcacgctgg agacgtcgat cctgagaggg
     8041 ggcggcgcgg gcctggtctg cggaccagga taactaagca gaatttcttc tttcagtttc
     8101 gggggcggat cagcgagcat ccgcctcgtt caacccctgc ggagagaacc gacatgccca
     8161 tcgcaactcg cgcactcgcg gcccttgcgg ccgctgctgc gctcgctttg cccttttcgg
     8221 tgcaggccgc gcccgacgat cgccctgtgc gcgtatgggc ggccgcctgc gcggcctgcc
     8281 acggcaccga cggtcacgcc caggccggca tgacgagcct tgcaggacgg aacgccgacg
     8341 agctctacac gctgctcatc gagttcaagc gcgatgtgcg cccggcgacc gtcatgcatc
     8401 agcatgccaa gggctacacg gatgaagaac tgcaacggct ggcgcagtgg ttcgccgccc
     8461 aggcaaagcg ctgaggaggc gggcatgagc acaatcgatc gtcgcggttt catccggtcg
     8521 gccggtggcc tggcccttgc gctcggtgcc ggcgtgggcg tcggcgcatg cgcgaccccc
     8581 gtcggccgcg ccgcgccggg gcgcgtcgtc gtcatcggcg gcggcttcgg cggcgccacc
     8641 gccgccaagt atctgaagaa gtgggagccc cgcctcgagg tgaccctggt cgagcgcaac
     8701 cagaacttcg tctcctgccc gatcagcaac ctggtgctgg cgggtgtcaa gcggatcgag
     8761 gacatcaccc tcgcctacga tggcctggcc cggtacggcg tgcgcctggt gcgcgacgag
     8821 gcggtggcca tcgacgtgga caagcgcgaa gtccgtctgg ccaagggcga tccgctgccc
     8881 tgggaccggc tcgtcgtcgc gcccggcatc gactttctct acgagcagat cccgggcctg
     8941 aacaacgccg acgcccgcca gcaggtgctg cacgcgtgga aggccggtgc gcagaccgtg
     9001 gcgctgcgca agcgcctgga agaactgccg gatggcgggg tcttcgcgat ctcgatcccc
     9061 aaggcgccct accgctgccc gcccgggcct tacgaacgcg ccagcgtcgt cgcccactac
     9121 ttcaagcagc acaagccgcg ctccaaggtg ctgatcctgg acggcaaccc cgagatcacc
     9181 tccaagaagg cgctgttcct gcaggcctgg gaggagcgct acaagggcat catcgaatat
     9241 cgccccgaca gcgtgctgca ggacttcgac gtgcgcgcca atgtcgggaa gttcgagttc
     9301 gagagcgtcc gcgccgagct cttcaacgtc atcccgccgc agcgcagcgg cctcatcgcc
     9361 agccccttcg ccaatgtggg cgggcgctgg gcgggggtcg atttccgcag cttcgagtcg
     9421 accgcggtgc cgggcgtgca cctgatcggc gatgccatcg ccaccgcgcc cgggatgccc
     9481 aagtcgggct tcatggccaa caaccacgcc aaggtggcgg cggatgcggt catcgccagc
     9541 ctcaccgggc tggagccgaa cccgacgccg atcatcgcca acacctgcta cagcttcgtc
     9601 tccgaccgcg acgtcgtgca cgtggcgtcg atccacaagt tcgacacgca ggagaacacg
     9661 ctgctgccgg tcaagggcgc gggcgggctg tcggcagtgg ccaacgatct ggaaggcaag
     9721 tacgcgctgg cgtgggcacg caacatctgg gccgacgcct tgacctgaca ggtgggcccg
     9781 atcgggcccg tgcatgagga gaccagaaaa tggatcaaga tccgggcgga ccatcgcgcg
     9841 cgctggcgga gcgtacccga gtctttctgg accgcctgcc actggcgcgg gatggcgggc
     9901 ggcaactccg ccgtctgccg gaaggtggtg tggtcggcga ctggttcggc tgcgagttgt
     9961 cgagtgtctt ccagcctatc gtcatccccg gcaccggggc catcacgggc tacgaggcct
    10021 atctgcgcat cctgggcagc ggcgagcggt cgctttcgcc gtggaccctg ttttcggcca
    10081 acgccgacga cgaccgcctg attgcgctcg atcgtctggc gcgtaccgcg catgcgctga
    10141 acttcgtgac ctcggtggac cgcgacagcc tgttgttcct caacgtgcac gggcgcctgc
    10201 tcgccgccgt cagcggcgat cacggtgccg cgttccgcaa ggtggtcgat gccctcggcc
    10261 tggcgccgga gcgcatcgtc atcgaaacgc cgctcgctgc cagcagccag cccgagctgc
    10321 tcgccttcgt gctgcgcaac taccgcaaca atggtttcca ggtggcgatc aacgtcgatt
    10381 cgccgatgca gtggcaagcg ctctccagcc tggtgccggc tcagttcgtc aagatcgaca
    10441 gcgcgcgact gttcattgcc gacgactggc gggcgcgcct ggaatggctg gcaacgctgc
    10501 gcgagcgtgc ggacatcgtc gtcacgcggc tggagcgcaa actggacggt gccctgcccg
    10561 agggcgtgca cctccagggc catgcctatg gcctgccggc gcagcgggcg gcgatcatgc
    10621 gcgccgaacg ccatgtgctg gaccgcttcg ccttctgagt caccgagagt cgagcaatgg
    10681 ccgcatcgtg tccgcaggaa atcttcatca ttggtggtgg cttcagcggt acgctcaccg
    10741 ccgtccatct gctgcgcgag gcgaggccgg ggcagttgcg tgtcacgctg atcgaacagc
    10801 atcggcgtgc cgcgcgcggg ttggcgtacc gcatctggga cgacagcatg ctgctcaacg
    10861 tgccggcggg caacatgagc gcgctcgccg atgagccggg gcatttcctc gcctactgtc
    10921 gcgacatcga tcccgcgttc aatgagggat cgttcgtgcc gcggcggctt tttggcgact
    10981 atctcgagca cacgctgcag gtcgccgagc gggggagccg cacgcccttc acccgcattc
    11041 gcgcccaggc cgttgcggta cgccgaaaac aggcctcgcc acttctgcag atcgaactgg
    11101 acgatggcgt cacgcggcag gccgatcagg tggtgcttgc gctcgggcat ctgggcgcgc
    11161 agcccttgca cttcgccgat gccctcgccg gctcgagtcg ctatatcggc aacccatggg
    11221 attatgcggc gatggaccag atccccgcgg gcggacctgt tgtggtcctt ggcaccggac
    11281 acaccgcgat cgatgccctg tttcgtctca ccagtcggga cgatgagcgc aaggtgtttc
    11341 tgctgtcgcg acgtggtttg ctgccaaagg gccaccgtac gcttccgcaa ccgccgccaa
    11401 cgacgacttt cccgggctat ctcgaagacc tgccgccgac cacctttgcc tacttgcgag
    11461 ccatacggcg ccaggtccgc cttcggcagc gccagggctg caactggcgc gacgtcgtca
    11521 acgccttgcg cccgcatacg ccggagatct ggcgccgcct gccggagatg gagcgccggc
    11581 gttttctctc gcgtgtaggc ccgtggtggg acatccatcg ccatcgcctg gcacccaccg
    11641 cttaccggcg cctgcgcgga atgctggcgt ccggtcaggc cgcggtcgtt gcggggcagg
    11701 ttctgcagat gcacgagcac ggaccgggcg tgcgggtcat cgtcaggccg cgtggcggga
    11761 gcgaggcgcg cgagtgggac gtcgccgcgg tggtgaactg caccgggcct gactacgaca
    11821 tcgaccgtct caccgcttcg cccttggtcg cacaactgcg cgacgagggc tgcgtgcgcg
    11881 cggatcctct gcacatcggc atcgatgtgg acgccggcta ccatgtggtc ggtgcagacg
    11941 gtctcaaggt ctcgggcctg cactacgtcg ggcccatgct tcgcgcgcgc tactgggagg
    12001 ccatcgcggt gcccgaactg cggcgacacg cgcacaacct ggcgcgtcac ctgctgacgc
    12061 cggggcaggc atgagccaca agtaggcggc ttcttcctgc cggcgcaggg cccctcagag
    12121 cagggcctga acgagcccga cggtggcgca tgccgcaatc accttcatga catcctgctt
    12181 gtacttccac agcgcgatga aggc
//
LOCUS       DAOQNN010000049        11650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2511_length_11650_cov_9.157137, whole genome shotgun sequence.
ACCESSION   DAOQNN010000049 DAOQNN010000000
VERSION     DAOQNN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11650
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2511_length_11650_cov_9.157137"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2181
                     /locus_tag="PKV97_04825"
     CDS             <1..2181
                     /locus_tag="PKV97_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HNB05234.1"
                     /translation="LAPGETAVSALRIHGERGVELVAEGEAELQRDDTVLTAERVTYR
                     EPTDEVLAEGDVVLSRGGDTIRGPKATLVVGDRVGSFETPTYELSRARPPALAGDAPR
                     TVAGSGEADLLRLEGENQYRLANATWTTCSPQDPDWYIRARELELDYDREVGTARGSS
                     IVFMDTPIFWMPWAEFPLSGQRQSGLLPPTFGSSNKTGVDFTQPYYWNIAPNYDATLA
                     PRIMTRRGVQFGGELRYLGEDYQGTTRLEWMPNDKVTGEERRLGSIQHQHRFAPNFYG
                     TLDLNAVSDDAYFEDLSSNIGVASRVNLLREGRLIYAGGWWTASALAQRYQTLNPDPD
                     VRNVEPYRRLPQLRLDAVRADLAGGMAGLLETEFVRFAHRERDRDEASRFVAYPQLSL
                     PMQGAAWFVTPKAGVHYTSYDIDRAVPKAGKPDSITRSVPIVSLDSGLFFERESHYFG
                     KDYLQTLEPRLFYLRVPSRNQDDIPLFDSNRFDVGFAQIFAENRYSGSDRIGDANDLT
                     AAVTTRFIDAETGVERLRALIGQRYYFSDQEVYLYNEDELRRAGTGNEILAGLGGRVT
                     STVTVDSYAQYNTETSRTERLNASVRYQPGFARTLNLSYRYAPNLRVDDDIVGLKDID
                     VSGQWPLARNWYGVGRVTHSLKDDRITEAVAGIEYDGGCWVFRTAVHRFAIDEDDVTN
                     AIFVQLELNDLAGIGSNPLSLIKRSVPGYGKINDSSANRVFGED"
     gene            2213..3529
                     /locus_tag="PKV97_04830"
     CDS             2213..3529
                     /locus_tag="PKV97_04830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB05235.1"
                     /translation="MKQLLSRTRVAIAIGLAAATLALPGHAAPRAVEVDRIVAVVNNE
                     VITGLELRSRIEQTRRQLARQGVELPPEDVLQRQLLERLIVERAQLQLARESSLRVDD
                     ATLDRAIERIASNNKLSIEQLRATLEKDGVTWSRFRDEIRSEILLTRLREREVDSRIV
                     VTDAEIDNFIANNPDAFSGQEFAVAHILLRTPEGASPQQVEAVARRAEQVMARLRSGE
                     DFARVAAEVSDAPDGLQGGGLGWRPLDRLPALFADAVRRMRPGETSPVLRSAAGLHIV
                     RLVDARGGGAAAVQQLEQTRARHILIKTSEVLSDADAEARLLGIRERVINGADFAELA
                     KASSADLSAARGGDLGWLNPGDTVPEFERAMNALRPGEVSAPVRSPFGWHLIQLVERR
                     MQDVTDERKRAAARQALRERKAEQAYEDWLRQLRDSTYVDYRIERE"
     gene            3526..4542
                     /gene="pdxA"
                     /locus_tag="PKV97_04835"
     CDS             3526..4542
                     /gene="pdxA"
                     /locus_tag="PKV97_04835"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNB05236.1"
                     /translation="MNVAAEAGRRLVAITSGEPAGVGPELCLRLAERAWPLRPVVLAD
                     IGLMRARAAALDSAVGVVEFDAARPAPEGAVEVLHLPLAAPSRAGVLDPANGAYVLRL
                     LDRAIAGCLQGEFAAMVTAPVHKGVICEGLGAHETMPFTGHTEYLAEHTGTPRVVMML
                     VGGGLRVALATTHLPLAAVPAAITPQVLEETLRILHADLRRHFGLAAPRILVAGLNPH
                     AGEGGHMGREEIDVIIPVLERLCAEGMQLVGPLPADTLFVPHTLAQGDAVLAMYHDQG
                     LPVLKHASFGGGVNVTLGLPIIRTSVDHGTALDLAGSGRADPGSLFAALELAIAMTQA
                     RAAA"
     gene            4586..5404
                     /gene="rsmA"
                     /locus_tag="PKV97_04840"
     CDS             4586..5404
                     /gene="rsmA"
                     /locus_tag="PKV97_04840"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNB05237.1"
                     /translation="MHDFDTDGHRARKRFGQNFLSDPNIIRKIIDGIRPQPGELMVEI
                     GPGLGAMTDPLIERLGHLHVVEIDRDLIARLHERYTPAQLTVHEGDALKFDFGSLCAG
                     EEGGVGPRLRIVGNLPYNISTPILFHLAGFADQVKDMTFMLQKEVVMRMVAEPGTEEY
                     GRLSVMLQYRFRMGRLFDVPPGAFRPAPKVMSSIVRMAPLPAEQLGARDEALLGRIVA
                     AAFGQRRKTLRNTLREFIDDAGLQALGIDPGLRGEKLSVEQYVAIANACAGRQG"
     gene            complement(5472..5663)
                     /locus_tag="PKV97_04845"
     CDS             complement(5472..5663)
                     /locus_tag="PKV97_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004354734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNB05238.1"
                     /translation="MKTNVGGADKILRIVAGIALLAWAVFGGPVWAWIGIVPLATGLL
                     GWCPAYTMLGMNTCPLGKK"
     gene            complement(5809..6495)
                     /locus_tag="PKV97_04850"
     CDS             complement(5809..6495)
                     /locus_tag="PKV97_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB05239.1"
                     /translation="MSASTSPTDAGRLEARYPVLAELSAPARKHLLQSAHWMHVPAGA
                     MLFDDHQACEGFPFVVEGTVRVSKCAPSGRELPLYRVGPGETCIISSSCLLGHEDYNA
                     RGVTESDTLLMLLPKAVFDDMMGERPFRDFVFHLFAERIADLMQLIEEVAFRKLDQRL
                     AALLLGKGRVLHVTHQQLADELGSVREIVSRLLKGFAEQGLVKLAREQVEIIDPAGLR
                     RMAAEGRGAA"
     gene            complement(6500..7264)
                     /gene="xth"
                     /locus_tag="PKV97_04855"
     CDS             complement(6500..7264)
                     /gene="xth"
                     /locus_tag="PKV97_04855"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNB05240.1"
                     /translation="MKIATWNVNSLKVRLPQVLDWLAASQPDALCLQELKLEDKAFPL
                     AELEAAGYHAVFIGQKTYNGVAILSPQPIDAATVVRNIPGFEDEQKRIIAATIAGVRV
                     VCGYFPNGQAVGSDKFEYKLRWLAALTDWLREELKTHERLVLAGDFNIAPEDRDAHPD
                     WKDEIHVSEPERAAFRALQDLGLIDAFRLFEQAERAFSWWDYRMMAFRRNFGLRIDHL
                     LVSPALRAACRSCVVDKEPRRLERPSDHAPVVLELA"
     gene            complement(7334..7711)
                     /locus_tag="PKV97_04860"
     CDS             complement(7334..7711)
                     /locus_tag="PKV97_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05241.1"
                     /translation="MDDIWMRILDNMSDRVSGPLKFRLLLQPLMAAIFAIKAGLLDAR
                     TGKPPYFWALATSPEHRVDMLKDGWKSVGKVFILAVVLDVVYQIIVLRFVYPGEVILV
                     AFTLAILPYLLLRGLVTRIARRR"
     gene            complement(7746..8480)
                     /locus_tag="PKV97_04865"
     CDS             complement(7746..8480)
                     /locus_tag="PKV97_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05242.1"
                     /translation="MEAPSLLETGFAAVLLAAAFVVGERTHLLGRLLTPRSLTSFGGG
                     MAAAYVFLHVMPELHGARHAFTEAAAMALPYEGKAIYYLALAGFLSFYGIRHFHTRQL
                     SAREGKPDRLDFQVQIGGFAAYAWLMGYLLLRGLEAHHIAIGLYAVAMAFHCLAINRE
                     LSHEYGNDYRRTGRFILAGMALAGWASAALLDLPASLIPLLLAFLSGAVIMNSLILEL
                     PTEKDGRFWPFITGGLLYGLILLPLG"
     gene            complement(8489..8971)
                     /locus_tag="PKV97_04870"
     CDS             complement(8489..8971)
                     /locus_tag="PKV97_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HNB05243.1"
                     /translation="MGKFSGMLSGPRPGGPQATETKAVKALAHELKSSNELAEDRTLL
                     AIKRTVMGADRTLMAWLRTALSMISFGFTIYKVLEGLKGGDVGVVAPQYEPRTVGLFL
                     VGLGTLAMVMGTVEYWFSIKGMRPVASVAIWKRPSFIMALIISLVGLGVFVSIIAHLL
                     "
     gene            9302..11518
                     /locus_tag="PKV97_04875"
     CDS             9302..11518
                     /locus_tag="PKV97_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB05244.1"
                     /translation="MLNGTGAGAPDKEGGNLRAEEQGRATAAAVDDLSFLALASALSS
                     SLVRARGTQVDEQVRNALQRIAEFFDADRGILCAVAHADRRVLVRDCVHRVGANLPAS
                     GPDAAEIFPWLFDEVVVCGRVVSIPSIDRLPPAAVRDRAGLSGWQLRSALFVPVSHAD
                     AVEFVLGLGCSRDDPGWTDETIARLRLLGETLAGALRHQALEQKQADSLRFERLIADA
                     CARFAGAWSDQIDTEIERALKDVLDFTGADQAGFFATVETPGHLRLTHLNHAEGIVPA
                     EKQVVPYDRICPWLYDRVVNQRRLYCFHRWDEIPIEAEVDRRYLEMKGTRSGLYIPYV
                     VGGEVRHVFALISNTAEMRWAPVLVERIQALGGAFVSALARKSVFDALRRNEEGLREA
                     QRIAGLGSWEWDVQTDAVWTSPQTDLILGSKAGSFKAFMNRVPEDERPALTQAVEACL
                     SGGPDRQRIQHRILAPGGEERFVENHFERVPGGPASAPRIFGTLQDITLRRRDELELE
                     GLRAERWHADRVAQIAVMTSSLAHELSQPLTAILSNAQAGLRMLRAGALPAEEGEAIL
                     EDIVADDKRAAQVIESLRAMLRRRPTERQRVEIAQLVRDVMGLMHSELVMNDVRFELD
                     LLAEGCIEVDRAQIQQVLINLIMNGIEAMHELPPGGRLLSLTVTRDERGGVKFSVADV
                     GHGLDVEDPGELFQPYWTTKATGIGMGLAICRSIVEAQGGKIRGENRRGNSMRSSY"
BASE COUNT         1851 a         3987 c         4017 g         1795 t
ORIGIN      
        1 ctcgccccgg gcgagaccgc ggtcagcgcg ctgcgcatcc atggcgagcg cggggtcgag
       61 ctggtcgccg agggtgaggc cgagctccag cgcgacgaca ccgtgctgac cgcggagcgc
      121 gtcacctacc gcgagccgac cgacgaggtg ctcgccgagg gtgacgtggt gctgagccgg
      181 ggtggcgaca cgatccgcgg gccgaaggcc acgctggtgg tgggcgaccg tgtcggcagc
      241 ttcgagacac cgacctacga gctctcgcgg gcgcgtccgc ccgcgctcgc cggcgacgcc
      301 ccgcgcactg tggccggcag cggcgaggca gacctgctgc gcctggaggg cgagaaccag
      361 taccgcctcg ccaacgcgac gtggaccacc tgcagcccgc aggatccgga ctggtacatc
      421 agggcgcgcg agctcgagct cgactacgac cgcgaggtcg gcaccgcgcg cggcagctcg
      481 atcgtgttca tggatacgcc gatcttctgg atgccctggg ccgagttccc gctcagcgga
      541 cagcgccagt ccggcctgct gccgcccacc ttcggctcgt cgaacaagac cggggtggac
      601 ttcacccagc cttattactg gaacatcgca cccaactacg acgccaccct ggcgccacgc
      661 atcatgaccc gccgcggcgt ccagttcggc ggcgagctgc gctatctagg cgaggactac
      721 cagggcacga cccgcctcga gtggatgccg aacgacaagg tcaccggcga ggagcgcagg
      781 ctcggctcga tccagcacca gcatcgcttc gcgcccaact tctatggcac gctagacctc
      841 aatgcggtct ccgacgatgc ctacttcgag gacctgtcct ccaacatcgg cgtggcctcg
      901 cgggtcaacc tgctgcgcga ggggcggctg atctacgcgg gcggctggtg gaccgcctcc
      961 gcgctggcgc agcgctacca gacgctgaac ccggatccgg acgtgcgcaa cgtcgagccc
     1021 tatcgccgcc tgccgcagtt gcgcctggac gccgtgcgcg ccgacctcgc cggcggcatg
     1081 gccggcctgc tcgagaccga gttcgtccgt ttcgcccatc gcgagaggga ccgcgacgag
     1141 gcctcgcgct tcgtcgccta ccctcagctc tcgttgccga tgcagggcgc ggcctggttc
     1201 gtgacgccca aggcgggcgt gcattacacc agctacgaca tcgaccgtgc cgtgcccaag
     1261 gcgggcaagc ccgattcgat cacacgctcg gtcccgatcg tctcgctcga ctccggcctg
     1321 ttcttcgagc gcgagtcgca ctacttcggc aaggactacc tgcagacgct ggagccacgg
     1381 ctcttctacc tgcgcgtccc gagccgtaac caagacgaca tccccctctt cgacagcaac
     1441 cgcttcgacg tcggcttcgc gcagatcttc gccgagaacc ggtattcggg cagcgaccgc
     1501 atcggcgacg ccaacgacct caccgcggcg gtcaccacgc gcttcatcga tgccgagacc
     1561 ggggtcgagc gcctgcgcgc gctgatcggc cagcgctact acttctcgga ccaggaagtc
     1621 tatctctaca acgaggacga gctgcgccgt gccggcaccg gcaacgagat tctcgccggc
     1681 ctcggcggac gcgtcaccag cacggtgacg gtggactcct acgcgcagta caacaccgag
     1741 accagccgca ccgagcgcct caacgccagc gtgcgctacc agcccggctt cgcgcgcacg
     1801 ctcaacctga gctaccgcta tgcgcccaac ctgcgcgtcg acgacgacat cgtgggcctc
     1861 aaggacatcg acgtctccgg gcagtggccg ctcgcgcgca actggtacgg tgtcggtcgc
     1921 gtcacccact cgctcaagga cgaccgcatc accgaggcgg tcgccggcat cgagtacgac
     1981 ggcggctgct gggtgttccg gaccgcggtg catcgcttcg cgatcgacga ggacgacgtc
     2041 accaacgcca tcttcgtgca actggaactg aacgacctcg ccgggatcgg ctcgaacccg
     2101 ctcagcctca tcaagcgcag cgttcccggc tatggcaaga tcaacgactc ctcggccaac
     2161 cgcgtcttcg gtgaagactg aggctgatcc cgcgcattca tctcccaacg acatgaaaca
     2221 actcctctcc cgcacccgcg tcgccatcgc tatcggcctg gcggccgcca cgctcgcgct
     2281 gcctggacac gctgcgccac gcgcggtcga ggtcgaccgc atcgtcgcgg tcgtcaacaa
     2341 cgaagtgatc accggcctcg agctgcgctc gcgcatcgag cagacccgcc gccagctcgc
     2401 ccgccagggc gtcgagctgc cccccgagga cgtgctgcag cgccagctgc tcgagcgcct
     2461 gatcgtcgag cgcgcccagc tacagctcgc gcgcgagtcc tcgctgcggg tggacgacgc
     2521 cacgctcgac cgtgcgatcg agcgcatcgc ctcgaacaac aagctctcga tcgagcagct
     2581 gcgtgcgacg ctggaaaagg acggcgtcac ctggagccgc ttccgcgacg agatccgcag
     2641 cgagatcctg ctcacccgcc tgcgcgagcg cgaggtggac agccgcattg tggtcaccga
     2701 cgccgagatc gacaacttca tcgccaacaa ccccgacgcc ttctccggcc aggagttcgc
     2761 cgtcgcccac atcctgctgc gcacgcccga gggcgcctcg ccgcagcagg tcgaggcggt
     2821 ggccaggcgt gccgagcagg tgatggcgcg gctgcgatcg ggcgaggact tcgcccgcgt
     2881 cgccgccgag gtctccgacg cgcccgacgg cctgcagggc ggcggcctgg gctggcgccc
     2941 gctcgatcgc ctgcctgccc tgttcgccga cgccgtgcgc cgcatgcgcc cgggcgagac
     3001 ttcgccggtg ctgcgcagcg ccgccggcct gcacatcgtg cgcctggtcg acgcgcgcgg
     3061 cggcggtgcg gcggcggtgc agcagctcga gcagacgcgc gcgcgccaca tcctgatcaa
     3121 gacctccgag gtgctctccg acgccgacgc cgaggcccgc ctgctgggca tccgcgagcg
     3181 cgtgatcaat ggcgccgact tcgccgagct ggccaaggcg agctcggccg acctctccgc
     3241 cgcccgcggc ggcgatctcg gctggctcaa cccgggcgac accgtgcccg agttcgagcg
     3301 cgcgatgaac gcgctgcggc cgggcgaggt cagcgcgccg gtgcgctcgc ctttcggctg
     3361 gcatctgatc cagctcgtgg agcgccgcat gcaggacgtc accgacgagc gcaagcgcgc
     3421 cgccgcgcgt caggccctgc gcgaacgcaa ggccgagcag gcctacgagg actggttgcg
     3481 ccagctgcgc gacagcacct acgtcgacta ccgcatcgag cgcgaatgaa tgtcgccgcg
     3541 gaagcggggc gcaggctcgt cgccatcacc agcggcgagc ccgcgggagt cggccccgag
     3601 ctgtgcctgc gcctggccga gcgcgcctgg ccgctgcgcc cggtggtgct cgccgacatc
     3661 gggttgatgc gtgcgcgagc cgcggcgctc gacagcgctg tgggcgtggt cgaattcgac
     3721 gccgcgcggc ctgcgcccga gggcgcggtc gaggtgctgc atctgccgct cgctgcgcct
     3781 tcccgggccg gcgtgctcga tccggccaac ggcgcctacg tgttgcgctt gctcgaccgc
     3841 gcgatcgccg gctgcctgca gggcgaattc gccgccatgg tcaccgcgcc ggtgcacaag
     3901 ggcgtgatct gcgagggtct cggcgcccac gagaccatgc ccttcaccgg ccacaccgaa
     3961 tacctcgccg agcacaccgg cacgccgcgg gtggtgatga tgctggtggg cggcggcctg
     4021 cgcgtcgcgc tggcgaccac ccacctgccg ctcgccgcgg tgccggcggc gatcacgccg
     4081 caggtgctgg aagagaccct gcgcatcctg cacgccgacc tgcgccgcca cttcggcctc
     4141 gccgcgccgc gcatcctggt cgccggtctc aacccgcacg cgggcgaggg cggccacatg
     4201 gggcgcgagg agatcgacgt catcatcccg gtgctcgagc gcctgtgcgc cgagggcatg
     4261 cagctggtcg gcccgttgcc cgccgacacc ctgttcgtgc cgcacacgct ggcgcagggc
     4321 gatgcggtgc tggcgatgta ccacgaccag ggcctgccgg tgctcaagca cgccagcttc
     4381 ggcggcggcg tcaacgtcac gctcggcctg ccgatcatcc gcacttccgt agaccacggc
     4441 accgcgctcg acctcgccgg cagcggccgc gccgatccgg gcagcctgtt cgccgcgctc
     4501 gagctggcga tcgcgatgac gcaggcgcgc gccgccgcct gagcgcggcg cagccaggcc
     4561 gggctgcccc ggcgaaagga aatccatgca cgacttcgac accgacggcc atcgcgcgcg
     4621 caagcgcttc ggccagaatt tcctctccga ccccaacatc atccgcaaga tcatcgacgg
     4681 catccgcccg cagccgggcg agctcatggt cgagatcggc cccggcctgg gggcgatgac
     4741 cgacccgctg atcgagcgcc tcggccacct gcacgtggtc gagatcgacc gcgacctcat
     4801 cgcccgcctg cacgagcgct acacgccggc gcagctcacg gtgcacgagg gcgacgcgct
     4861 gaagttcgac ttcggcagcc tttgcgccgg cgaggagggc ggcgtcgggc ccaggctgcg
     4921 catcgtcggc aacctgccct acaacatctc cacccccatc ctgttccacc tcgccggctt
     4981 tgccgaccag gtcaaggaca tgaccttcat gctgcagaag gaggtggtga tgcgcatggt
     5041 ggcggagccc ggcaccgagg agtacggacg gctgtcggtg atgctgcagt accgcttccg
     5101 catggggcgc ctcttcgacg tgccgccggg cgccttccgt ccggcgccca aggtgatgtc
     5161 cagcatcgtg cgcatggcgc cgctgccggc ggaacagctc ggcgcgcgcg acgaggcctt
     5221 gctcgggcgc atcgtcgccg ccgccttcgg ccagcgccgc aagaccctgc gcaacacctt
     5281 gcgcgagttc atcgacgacg ccgggctgca ggcgctcggg atcgaccccg ggctgcgtgg
     5341 cgagaagctg tcggtggagc agtacgtcgc gatcgcgaat gcgtgcgcgg ggcgacaggg
     5401 gtgaggcgcc aagtcgagag gcgcgtaaaa aagccggcgg tgcgccggct ttttcttggg
     5461 gtggggcgtg cttacttctt gccgagcggg caggtgttca tgcccagcat cgtgtaggcc
     5521 gggcaccagc cgagcaggcc ggtggcgagc gggacgatgc cgatccaggc ccacaccggg
     5581 ccgccgaaca ccgcccaggc gagcagcgcg ataccggcca cgatgcgaag gatcttgtcg
     5641 gcgccaccaa cgttcgtttt catgtgtgtt ctccttggat cggatgaggt gagtggccgg
     5701 attggaccac gcccggccgg ctgcggtctg taaccgaggt tacacagccc gtacccgatc
     5761 cgggatgcta cgccggtccg cccgggagcg ggatcccgga aggggggctc aggccgcgcc
     5821 ccggccctcg gcggccatgc ggcgcagccc cgccgggtcg atgatctcga cctgctcgcg
     5881 cgcgagcttg accaggccct gctcggcgaa gcccttgagc aggcggctga cgatctcgcg
     5941 cacgctgccg agctcgtcgg cgagctgctg gtgggtgacg tgcagcacgc ggcccttgcc
     6001 gagcagcagc gccgccaggc gctggtcgag cttgcggaag gcgacctcct caatcaactg
     6061 catcaggtcg gcgatgcgct cggcaaacag gtggaagacg aagtcgcgga aggggcgttc
     6121 gcccatcatg tcgtcgaaca ccgccttcgg cagcagcatc agcagggtgt cggactcggt
     6181 gacgccgcgc gcgttgtagt cctcgtggcc gagcaggcag gacgaggaga tgatgcaggt
     6241 ctcgcccggg ccgacgcggt agagcggcag ctcgcgcccg ctcggcgcgc acttgctcac
     6301 ccgcaccgtg ccctcgacca cgaagggaaa gccctcgcag gcctgatggt cgtcgaacag
     6361 catcgcgccc gcgggcacgt gcatccagtg cgcgctttgc agcaggtgct tgcgcgccgg
     6421 tgccgacagc tcggcaagca ccgggtagcg ggcttcgagg cgcccggcgt cggtcgggga
     6481 ggtgctggcg ctcatcgctt caggccagtt ccagcaccac cggggcgtgg tccgacggcc
     6541 gctcgaggcg gcgcggttcc ttgtcgacca cgcagctgcg gcaggccgcg cgcaacgccg
     6601 gcgacaccag caggtggtcg atgcgcaggc cgaagttgcg ccggaaggcc atcatccggt
     6661 agtcccacca cgagaacgcg cgctcggctt gctcgaagag gcggaaggcg tcgatcaggc
     6721 cgaggtcctg cagcgcgcgg aaggcggcgc gctcgggctc cgacacgtgg atctcgtcct
     6781 tccagtcggg gtgggcgtcg cggtcttccg gtgcgatgtt gaagtcgccg gccagcacga
     6841 ggcgttcgtg cgtcttcagc tcctcgcgca gccagtcggt cagcgcggcg agccagcgca
     6901 gcttgtactc gaacttgtcc gagcccaccg cctggccgtt gggaaagtag ccgcacacca
     6961 cgcgcacacc ggcgatggtg gcggcaatga tgcgcttctg ctcgtcctcg aagccgggga
     7021 tgttgcgcac caccgtggcc gcgtcgatcg gctgcgggct caggatggcg acgccgttgt
     7081 aggtcttctg gccgatgaag acggcatggt agccggcagc ctcgagctcg gcgagcggga
     7141 aggccttgtc ctcgagcttg agctcctgca ggcacagcgc gtcgggctgg ctggcggcga
     7201 gccagtcgag gacctggggc aggcggacct tgagcgagtt gacgttccag gtggcgatct
     7261 tcatctgtcg taccggcgat tgaagcaagg cccgccattg taagccgcca agtcacaagt
     7321 cactggctcg cgatcagcgc ctcctggcaa tgcgggtgac cagcccgcgc agaagcaggt
     7381 agggcaggat cgccagcgtg aaggcgacga ggatgacttc gcccggatac acgaagcgca
     7441 gcacgatgat ctggtacacg acgtcgagca cgacggcgag gatgaacacc ttgccgacgc
     7501 tcttccagcc atccttcaac atgtctaccc ggtgctccgg gctggtcgcc agcgcccaga
     7561 agtagggcgg cttgccggtt cgggcatcga gcagcccggc cttgatggcg aagatcgccg
     7621 ccatgagcgg ttgcagcagc aggcggaact tcagcgggcc gctcacgcga tcgctcatgt
     7681 tgtcgaggat gcgcatccag atgtcgtcca tttctggttt ctccgtaaaa agaggaaggt
     7741 cgccgtcagc ccagcggcag caggatgagg ccgtagagga ggccgcccgt gatgaagggc
     7801 cagaagcggc catccttttc cgtcggcagt tccaggatca ggctgttcat gatcacggcg
     7861 cccgacagaa aggcgagcag cagcgggatc aggctcgccg gcaggtcgag cagcgccgcg
     7921 cttgcccagc cggcgagggc catgcccgcg aggatgaagc gaccggtgcg gcggtagtcg
     7981 ttgccgtatt cgtggctcag ttcgcggttg atcgcgagac agtggaaagc catggcgacc
     8041 gcgtacaggc cgatggcgat gtgatgcgct tcgagcccgc gcagcagcag atagcccatc
     8101 agccaggcgt aggcggcgaa gccgccgatc tggacctgga agtccaggcg atccggcttg
     8161 ccctcgcgcg cgctcaactg gcgcgtatgg aagtgccgga tgccgtagaa gctcaggaat
     8221 ccggccaggg ccaggtagta gatcgccttg ccttcgtacg gcagcgccat ggcggcagct
     8281 tccgtgaacg catggcgtgc gccgtggagt tccggcatga cgtgaagaaa gacgtaggcg
     8341 gccgccattc ctccgccgaa cgaggtgagg ctgcgcggcg tgagcagccg gccgagaagg
     8401 tgcgtgcgct cgccgacgac gaaggcggcc gcgagcagta ccgccgcgaa acccgtttcg
     8461 aggagactgg gggcttccat gccggtactc agagcaggtg cgcgatgatg ctcacgaaca
     8521 cccccaggcc cacgagggag atgatcagcg ccatgatgaa cgacgggcgt ttccagatgg
     8581 cgaccgaggc gacgggccgc atgcccttga tcgagaacca gtactcgacg gtgcccatga
     8641 ccatcgccag cgtgccgagg ccgacgagga agaggccgac ggtgcgcggt tcgtattgcg
     8701 gggcgaccac accgacgtcg ccgcccttga gcccttcgag caccttgtaa atcgtgaagc
     8761 cgaagctgat catcgacaat gcggtgcgca gccaggccat cagcgtgcga tcggccccca
     8821 tcaccgtgcg cttgatggcg aggagggtgc ggtcttcggc gagttcgttg gacgacttga
     8881 gctcgtgcgc gagcgccttg accgccttgg tttcggtagc ctgggggccg ccgggtctgg
     8941 gacctgaaag catgccgctg aactttccca tcagggacgg gtgggatggc ggcgtcttgc
     9001 cgctcgtggc cggttcgccg tccgctggtc gcttgtcgtc gtctgccaca tcggactcct
     9061 gcacagaggg ccatctgctt gtgataaaag cggatcttgc ggtgatccga gcgcatcgaa
     9121 cccgcagcgc ggcgcccggc gtgcgcatcg ataggcccgc gaatcgcatt gctcgcacct
     9181 tacgcgcagc gggacatgga gggcattgga ccttgggctt agtcgggctc cggcctggcg
     9241 cgcgcctctg cgtccggcgg gctcgtcaat cgggtcgctt tcttacgggg tcttggcatc
     9301 gatgttgaac gggacgggag ccggcgcgcc tgacaaggag ggcggcaatc tgcgcgccga
     9361 ggagcaaggg cgtgcgactg ccgctgccgt ggacgatctc agctttctag cgctggcgtc
     9421 ggccttgtcc tccagcctcg tgcgtgcccg cggcacccag gtcgacgagc aggtccgcaa
     9481 tgccttgcag cgcatcgccg agttcttcga cgcggatcgg ggcatccttt gcgccgtggc
     9541 gcacgcggac cgccgcgtgc tcgtccgcga ctgcgtgcac agggttgggg cgaacctgcc
     9601 cgcgagcggg ccggatgcgg cggagatctt cccctggctc ttcgacgagg tcgttgtctg
     9661 cggccgggtg gtgagcattc cgtcgatcga tcgccttccg cccgcggcag tgcgcgatcg
     9721 tgccgggctg agcggatggc agctgcggtc cgcgctcttc gttcccgtct cgcatgcgga
     9781 cgcggtggag ttcgtgctgg ggctggggtg ctctcgtgac gatccgggct ggaccgacga
     9841 gacgatcgcc cgcctgcgtc tgctcgggga aacgcttgcc ggtgcgctgc ggcatcaggc
     9901 cctcgaacag aagcaggccg actcgctgcg cttcgagcgg ctgatcgccg atgcgtgcgc
     9961 gcgcttcgca ggagcgtggt cggatcagat cgacacggag atcgagcgcg ccctgaagga
    10021 cgtgctcgat ttcaccgggg ccgatcaggc cggatttttc gccacggtgg agacccccgg
    10081 gcatctccgg ctgacccatc tgaaccatgc ggagggcatc gttccggcgg aaaagcaggt
    10141 ggtgccctat gatcgcatct gcccctggtt gtatgacagg gtcgtgaatc agcgccgcct
    10201 ttattgcttc caccgctggg acgagatccc catcgaggcc gaggtcgatc gtcgctatct
    10261 cgagatgaag gggacgcgct cgggcctcta cattccctac gtcgtcggcg gtgaggtgcg
    10321 gcacgtcttt gcgctgatca gcaacaccgc cgaaatgcgc tgggcgccgg tgctcgtcga
    10381 gcgcattcag gcattggggg gcgcttttgt gagtgcgctg gcgcgcaaaa gcgtcttcga
    10441 cgccctgcgg cgcaacgaag aaggcctgcg cgaggcgcag cgcatcgcgg ggctcggcag
    10501 ttgggagtgg gacgtgcaga ccgatgcggt gtggacctcg ccgcagaccg acctgatcct
    10561 cggctcgaaa gcggggtcgt tcaaggcctt catgaacagg gtccccgaag acgagaggcc
    10621 tgcattgacg caggccgtcg aggcctgcct gtcggggggg cctgaccgcc agcgcatcca
    10681 gcaccgcatc ctcgccccgg gtggggaaga gcgtttcgtc gagaaccatt tcgagcgggt
    10741 tccgggcggc ccggcgtccg ccccgcgcat cttcggcacg ctgcaggaca tcacgcttcg
    10801 ccgccgcgac gaactcgagc tcgagggcct gcgcgcggaa cgctggcatg ccgatcgcgt
    10861 cgcgcagatc gcggtgatga cctcttcgct ggcgcacgaa ctgagccagc cgcttaccgc
    10921 catcctcagc aatgcgcagg ccgggctgcg aatgctccgg gccggtgcct tgcccgccga
    10981 ggaaggcgag gcgatcctcg aggacatcgt ggccgacgac aagcgtgccg ctcaggtcat
    11041 cgaatccctg cgtgcgatgc tgcgccgccg gcccaccgag cgccagcgcg tggagatcgc
    11101 gcaactggtg cgggacgtca tgggcctgat gcatagcgaa ctggtcatga acgatgtgcg
    11161 gttcgagctc gatctgcttg ccgagggctg catcgaggtc gatcgggcgc agatccagca
    11221 ggtcctcatc aacctgatca tgaacggcat cgaggccatg cacgagcttc cgcccggcgg
    11281 ccgcctgctg agcctgacgg tgacgcgaga cgagcgtggg ggagtgaagt tttccgtcgc
    11341 ggacgtcggc catgggctgg acgtcgagga cccgggcgag ttgttccagc cctactggac
    11401 gaccaaggcg acgggcatcg gcatggggct tgcgatctgc cggtcgatcg tcgaggcgca
    11461 gggcgggaag atccggggcg agaacagacg tggaaactca atgagatcga gctactgaag
    11521 taggggaagt acaaagacgc ttcagataaa cgacccggcg acgcttgcgc gtcgccgatc
    11581 cggtcggcgc tcctggcgcc tgactgttat caacgtcggc aacgcgccca gtttatgcct
    11641 gcgccaccat
//
LOCUS       DAOQNN010000050        11544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2539_length_11544_cov_8.205414, whole genome shotgun sequence.
ACCESSION   DAOQNN010000050 DAOQNN010000000
VERSION     DAOQNN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11544
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2539_length_11544_cov_8.205414"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PKV97_04880"
     CDS             complement(<1..212)
                     /locus_tag="PKV97_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB05245.1"
                     /translation="MRFEDVRFRWSADRPMVFDGLDLDIPAGARIALLGPSGAGKSTL
                     AALALKVAAPVAGRITLGGVDLATLSA"
     gene            332..817
                     /locus_tag="PKV97_04885"
     CDS             332..817
                     /locus_tag="PKV97_04885"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05246.1"
                     /translation="MEVELVRADLFSSRASARLLVYGDDFNKAAWSAEHLRRIRCALA
                     DLATRFQADGRTVFMTALAPDKSSAYRPWARDPARLPAERLGELFDGLDVPDARLDLA
                     IRDAVERGVVDVYMPDDTHWSGAGHALAAQVIFERLSRLRAQADVATGEARAQGPEAP
                     K"
     gene            830..1648
                     /locus_tag="PKV97_04890"
     CDS             830..1648
                     /locus_tag="PKV97_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB05247.1"
                     /translation="MVKVLRAAVLLAGSIAMTGASAGTEADYQALRAWCVQRLQNQNN
                     AAWQAANNPQKYFHFHHFCFGMQWENKVVGSASQKERERYVGSIVNETSYIISHAARS
                     HFLMPEVYFLRGWAQYYGKQHIEAESALLNALQLDPRHARAAAQLATLYRATDRRAKA
                     AEVVRTALKLSPNDFRLRKMGEEFGVELPPLVRPPAPKPAAPAEPSQAEPADAAPATT
                     EATVTQEAATAARSAAQVDQDAGTAEGASQGCRFCPPAEIQKRWRESFGEPQKP"
     gene            1668..2630
                     /locus_tag="PKV97_04895"
     CDS             1668..2630
                     /locus_tag="PKV97_04895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB05248.1"
                     /translation="MSIDISAIVPVYRSQAMLPELHRRLVGALEQIGRPFEIILVEDC
                     GGDDSWSVIEQLAAADPRVLGLRLARNYGQHNALLCGIRAARGELVVTLDDDLQNPPE
                     EIHRLLARLDEGYDVVYGSPQAETHGFLRDQASRITKLALQGAMGVESASKVSAFRVF
                     RARLREAFAAYRSPSVNIDVLLTWGTTRFGSVLVRQDERAVGDSGYTLRKLINHAINM
                     MTGFSVLPLQVASVLGLAFGSMGFLVLLYVLLRYLVDGSAVPGFPFLASIIAIFSGVQ
                     LFALGIFGEYLARMHFRSMERPPYALRSSTAAQGDTETVHDQCC"
     gene            2614..3417
                     /locus_tag="PKV97_04900"
     CDS             2614..3417
                     /locus_tag="PKV97_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB05249.1"
                     /translation="MTSAAEAAPSHPLHGRIDTAALRLEWAEAPWDTAVFGHPVVQVS
                     RIEARTGASAADLAPFVAACEVGGVGLASCRLPHDRLRESMLLEEVGFRFIEMVFQPE
                     FGDLQSAGPRDAQGLVVERARPEDLEEIVEVAGGAFRNERFHVDPRLPSRLGDLRYQN
                     WVRSSLEHPSQRLHAVREGAELVAFFVTEQLADGTCYWHLNAVVPQRQGRGYGLRAWR
                     AMMNQARLEGATRVRTSIVARNHRVLNLYARLGFSFPAPSMTFHRVRVA"
     gene            3414..3836
                     /locus_tag="PKV97_04905"
     CDS             3414..3836
                     /locus_tag="PKV97_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNB05250.1"
                     /translation="MSAQATLGQFVRYATVGLASNVLLYLAYLALTATGVEAKLAMSV
                     LYLLGVVQTFVFNKRWSFRHGGMHGPAFVRYCLSYAAGYLLNLVALMVLVDRMGHPHQ
                     LVQGVSIVVLAILLFLLQKFWVFRPNSVPHSHSTGPQP"
     gene            3833..4957
                     /gene="rffA"
                     /locus_tag="PKV97_04910"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             3833..4957
                     /gene="rffA"
                     /locus_tag="PKV97_04910"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNB05251.1"
                     /translation="MNIPFNKPHMTGKELWYIAQAHANGHLAGDGNFTRQCSRWLEAR
                     TGASKALLTHSCTAALEMAAILADLEPGDEVIMPAYTFVSTANAFVLRGAVPVFVDVR
                     PDTLNIDERLIEAAITARTRAIVPVHYAGVACEMDTIMDIARRHDLLVVEDAAQAVMA
                     SYKGRPLGAIGHMGALSFHETKNIISGEGGALLVNAPGRAERAEIIREKGTNRSQFFR
                     GQVDKYTWVDIGSSYLPGELIAAFLWAQMEEADSITRRRLGIWNTYHQWFAEAERQGR
                     LRRPVIPAHCAHNAHMYYLLLPGLEQRTAFIERLRGLGIHCVFHYIPLHDAPAGRRFG
                     RAHGELSVTEDIADRLVRLPLWLGMEDAQAEIIQQVLAAL"
     gene            4989..>5860
                     /locus_tag="PKV97_04915"
     CDS             4989..>5860
                     /locus_tag="PKV97_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05252.1"
                     /translation="MSRHRACLPGAGGGCASGGGRGRVAGRTRVLWLAVGLAMLLVAL
                     SRAPLADVHWDAPIYLYQAKRFAETAYLVDFARNAQEIAAQLAGAWPEDESYSEAFWR
                     SSRLGHIALLGAPVAAFGSTDAAITAAHWMFVLAMPLGLLAWCRVSHLAGRALEPGAV
                     GAGGLASSALLFVLSDVHGYLSGNLVSEVLSFGLGAAAVWALLASTATAAGWLAAVSG
                     GLAFLGYTVRVESVWIWLAFMLAYLPFAGAAEARARTLRRFVLAGGTALLGYATYAFV
                     FHPLADPRSYLAFAA"
     assembly_gap    5861..5960
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5961..6554
                     /locus_tag="PKV97_04920"
     CDS             <5961..6554
                     /locus_tag="PKV97_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05253.1"
                     /translation="AAGWTFVLGARQRCAALGLAVVFVLMSRPPIYGWFVEQPGLWRI
                     QRLGAAMFVPRYERVDYVPREMAAISAQVFGGEGRPGILIASPSVKQEYLNLVRFYGP
                     AYPADAHLALAGDPTNPGRCELRRDRAHEPVRFCTGFGDAAALRASLVGHRVLHLRRV
                     GDPPLAGAAPLLQTTGFVLEQLGPLPVPDPSPEGSPP"
     gene            6551..8167
                     /locus_tag="PKV97_04925"
     CDS             6551..8167
                     /locus_tag="PKV97_04925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNB05254.1"
                     /translation="MRSWLPCALLFALAALFLAPALGGSGFWWTDETRHAMGGVFILD
                     LLRDLPLDDPMGYALRYFAQYPALALNWYLPGFYFVEAGFFALFGVSETVAHWTVFAF
                     VLLAAGLWFDWVRTGWNPLMAFMATALFIAVPEWNHWARSVMLEAPAIALLVLVVWTF
                     ERYLDRPAWSRAIAAGLAIAAALAVKQTVALLLPALLAYGLISSRRAALWRVQAVPAY
                     LIAALAIALVALHALKFGNLGLAATVGDSRVEVGQSAARLSLERWLTYPRALIEVWGW
                     PLALLSLAGALLPARQGEGRLALIHAWLLCWYVVMTLVLGGAGNAPRYTLYALPALAV
                     LAARPLFLLRGRRSHDGFLVLLVVMLAFQGWRTLTQPVPRIDGYREVAQAVHERRTGA
                     PVLFAGKHDGSFIFHLRQLDHAREAVVLRADKVLVSLAVHKYFGMSSHVSGIDDIRAL
                     IRRYGIEYVVIERPDIVGVPEFEMLAELLAQADFERVAEFPVRAAGGAEAPERIVLHR
                     YRDHVPAADAEIVIPLPHLGREIRFRRAGG"
     gene            complement(8120..11317)
                     /locus_tag="PKV97_04930"
     CDS             complement(8120..11317)
                     /locus_tag="PKV97_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNB05255.1"
                     /translation="MNVATAARSLTILICTHNRADLLARVIDSLESARQPAGWSVRLF
                     VVANACTDGTHEFLAGRSDRADRLALSWIAEPTPGKSHALNRALPLLEDELVAFVDDD
                     HRVDADYLLGVTAAAERWPEADLFCGRIVPDWDGSEPAWVHDEGPYRIYPLPVPRYDQ
                     GMEDFPIDLEGPIPGGGNLVARLPVIGATGPFAIELGPTGHDLGGSEDADWILRALRK
                     GARLHYAPQILQYHYVDSERLTLSYVARKGYQRSQSVTRVRAEFDRVPRYMWRKAAGY
                     ALGLAFSWRLQARRFYLVRLASSMGEISGIRDRHRRKRRRAALPMLGAEAGSAALLAA
                     AALTLALAAVLARHWLGEALLGAGVVGALTSAVLVAKSVRDFSRTGPGLREEILARYR
                     GYVVFAIARLGLAALGLAAFWAFPGTALWITAAEALGREPPLWTTAAGGALTLALATV
                     YAGCRALSQNPGLVIASWQYRTVRIHRLWRALSQRGLDLIARIVLATGIGLVGAIALL
                     RYHQGGSADAGAMLLVTCGYIALLAWAIWEPDGTHAPTPRRRARRNVLMIGSDTLRAD
                     RIGAQREGASITPNIDALAARGTRFGACYVPCARTAPSLISLFTATWPHHHGVRDNYV
                     AGAETRLEDKTLPNILRALGYRTAAVSDWCGADLGKFDFGFDITDLPEDQWNLKYLIR
                     QGPKDIRLFLSLFLHNRLGRHLLPEIHYLGGVPQTGMLGRRARRTLSRLAAGDEPFLL
                     NLFYSTTHPPFASEHPYYTRFSDPAYSGASKFAMARLTEPFEIIRRQGEPREEFDLDQ
                     ILDLYDGCVAQFDDEVGRLLRQLDDSGLAEDTIVVLYSDHGMEFFEHGTWGQGNSALG
                     DFSARVPLIVVDPARPGGQRVDQVVRSVDIMPTLLDLLGAPSVGCDGVSLRPAIADPA
                     TDLHLRAFNETGIWIAPVPGLPEGHLSYPNLLELLDVPDIAAGSLSLRERYRQTVLVA
                     KDRMVRDGRWKLVYQPLEHGRLLSLYDVENDPGCTADVASRHPAEVERLWAQLRAWMA
                     NDPALRGDPRLDLPPTPAATSAARAPEADLAPEMR"
     gene            complement(11314..>11544)
                     /locus_tag="PKV97_04935"
     CDS             complement(11314..>11544)
                     /locus_tag="PKV97_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB05256.1"
                     /translation="LAMTRKNRHLLPADKQGAYWRALYAGTLCDYAKWACREGDRGAA
                     LADLARAFACAPVGRGRLIASIALAVLAGGRL"
BASE COUNT         1616 a         3935 c         4026 g         1867 t
ORIGIN      
        1 gccgacagtg tcgccaggtc aacgccgccc agcgtgatgc gtcccgcgac tggcgccgcc
       61 accttcagcg ccagcgcggc cagggtcgac ttgccggcgc ccgaagggcc cagcagggcg
      121 atgcgggcgc cggccggaat gtccaggtcg agcccgtcga agaccatcgg ccggtcggcc
      181 gaccagcgga aacgcacatc ctcgaagcgc aacgcatgcc cgcgcggtgc cgcggccgca
      241 tgcgctggcg gaggccaacc ccgggttcgc gcgcagctgg ctctaccgca gtgccatgcg
      301 tggtctcttc ggtgtgcagg ccggcaacac ggtggaggtc gaactcgtgc gtgccgacct
      361 cttctcgtcg cgcgcatccg cccgcctgct ggtgtacggc gacgatttca acaaggccgc
      421 ctggagtgcg gaacacctgc gccggatccg ctgcgccctc gcagacctgg cgacgcgctt
      481 ccaggccgac ggacggacgg tgttcatgac cgcgctggcg ccggacaagt cgagcgcgta
      541 tcgtccctgg gcgcgcgatc ccgcccgact gccagccgag cggctgggcg agctcttcga
      601 cggactcgat gtgcccgacg cccggctcga cctcgccatt cgcgacgccg tggagcgcgg
      661 tgtcgtcgac gtctatatgc cggacgatac gcactggagc ggcgcagggc atgcgcttgc
      721 ggcgcaggtg atattcgagc gcctgtcgcg cttgcgtgcg caggcggatg tcgccacggg
      781 cgaagcgcgg gcgcaaggac ccgaagcgcc gaaatgacag gagacgatga tggttaaagt
      841 gctgcgagca gccgtgttgc ttgccgggtc gatcgcgatg acgggcgcga gcgccggaac
      901 ggaggccgac taccaggcgc tgcgcgcctg gtgcgtgcag cgcctgcaga accagaacaa
      961 tgccgcctgg caggcggcca acaatccgca gaaatacttc cacttccatc acttctgctt
     1021 cgggatgcag tgggagaaca aggtggtcgg ctccgcctcg cagaaggagc gcgagcgcta
     1081 cgtcggttcg atcgtcaacg agaccagcta catcatcagc catgcggcac ggtcgcactt
     1141 cctcatgccg gaggtctatt tcctgcgtgg ctgggcccag tactacggca agcagcacat
     1201 cgaggcggag tccgccctgc tcaatgccct gcaactggac ccccgccatg cacgggcggc
     1261 cgcgcagctg gccaccctgt accgggcgac cgatcggcgc gccaaggcgg ccgaggtcgt
     1321 ccgcacggcc ctgaagcttt cgccgaacga ctttcggctg cgcaagatgg gcgaggagtt
     1381 cggcgtcgag ctgccgccgc tggtgcgccc gcctgcaccg aagccggcag cgcccgccga
     1441 gccgtcgcag gcggagcctg cagacgccgc gccggccacc accgaggcga cagtgacgca
     1501 ggaggcggcg accgcggcgc gttcggcggc gcaggtcgac caggatgccg gcaccgccga
     1561 gggcgcttcc caaggctgcc ggttctgtcc gccggcggag atccagaagc gctggcgcga
     1621 gtcgttcggc gagccgcaga aaccctgacc gccccctgaa gacgaccatg tccatcgata
     1681 tttccgccat cgttccggtc tatcgctcgc aggccatgct gcccgaactg catcgacggc
     1741 tcgtcggcgc gctcgagcag atcggccggc cgttcgagat catcctcgtg gaagactgcg
     1801 gcggcgacga ctcctggagc gtcatcgagc agctggctgc ggccgatccc cgcgtgctcg
     1861 gcctgcgcct ggcgcgcaac tacggccagc acaacgccct gctgtgcggc attcgggccg
     1921 cgcgcggcga gctcgtcgtg acgctggacg acgacctgca gaatccgccg gaagagatcc
     1981 atcgcctgct ggccagactg gatgaaggct acgacgtcgt ctatggttcg ccacaggccg
     2041 agacgcacgg cttcctgcgc gaccaggcct cgaggatcac caagctcgcg ctgcaggggg
     2101 cgatgggcgt ggagtcggcg agcaaggtga gcgcgttccg cgtcttccgc gcccgcctgc
     2161 gcgaggcctt cgcggcctac cgcagcccgt cggtcaatat cgacgtgctg ctcacctggg
     2221 gcacgacgcg tttcggctcc gttctcgtcc gccaggacga gcgtgccgtc ggcgattccg
     2281 gctacaccct gcgcaagctc atcaatcatg cgatcaacat gatgaccggc ttctcggtgc
     2341 tgccgctgca ggtcgccagc gtgctcgggc tggctttcgg gtcgatgggc ttcctggtgc
     2401 tcctgtacgt gctcctgcgc tatctcgtcg acggaagcgc ggtgcccgga ttccccttcc
     2461 tggcctcgat catcgcgatc ttctcgggcg tccagctgtt cgcgctgggg atcttcgggg
     2521 agtacctggc gaggatgcat ttccgctcga tggagcgtcc gccctatgcg ctgcgatcct
     2581 ccaccgctgc gcagggcgac acggagactg ttcatgacca gtgctgctga agccgccccc
     2641 tcccaccccc tgcacggccg catcgacacg gctgcgctcc gcctcgaatg ggcggaggcc
     2701 ccctgggaca cggcggtatt cggccatccg gtggtgcagg tgtcgcgcat cgaagcccgc
     2761 acgggcgcca gcgcggcgga tctggcgccc ttcgtcgccg cctgcgaggt cgggggcgtg
     2821 gggctggcga gctgccggct cccccacgat cggctgcgcg aatcgatgct gctcgaggag
     2881 gtcggctttc gtttcatcga gatggtcttc cagcccgagt tcggcgacct gcagtcggcc
     2941 gggccgcgcg atgcacaggg actggtggtc gaacgagcgc gacctgaaga tctggaggaa
     3001 atcgtcgagg tggccggagg cgccttccgg aacgaacgtt tccacgtcga cccgcggctg
     3061 ccatcgcggc ttggcgacct gcgctaccag aactgggtgc gcagcagcct cgagcatccc
     3121 tcgcaacgcc tccatgccgt gcgcgaaggg gccgaactgg tcgccttctt cgtcaccgag
     3181 cagcttgccg acggcacctg ctactggcat ctcaacgcgg tcgtgccgca gcggcagggc
     3241 aggggctacg gcttgcgcgc ctggcgcgcg atgatgaacc aggcgcgcct cgaaggggcg
     3301 acccgggtgc gtaccagcat cgtggcgcgc aaccatcggg tgctcaacct ctatgcgcgt
     3361 ctcggcttca gcttcccggc gccctcgatg actttccaca gggttcgggt tgcatgagtg
     3421 cgcaagccac cctgggccag ttcgtccgct acgcgacggt ggggctggca tcgaacgtgc
     3481 tgctctacct ggcttatctc gcgctgacgg cgacaggcgt cgaggccaag ctcgccatga
     3541 gcgtgctcta tctgctcggg gtggtgcaga ccttcgtctt caacaagcgc tggtccttcc
     3601 gtcacggcgg gatgcacggg ccggcgttcg tgcgctactg tctctcctac gcagcgggtt
     3661 acctgctcaa cctggtcgcg ctgatggtgc tggtcgaccg catgggccat ccccatcagc
     3721 tcgtccaggg cgtgtcgatc gtcgtgctcg cgatcctgct gtttcttctg cagaagtttt
     3781 gggtcttccg tccgaattcc gttcctcact cgcactcgac cgggccgcag ccatgaacat
     3841 ccccttcaac aagccccaca tgacgggcaa ggaactctgg tacatcgccc aggcccacgc
     3901 caacggccac cttgcgggcg acgggaactt cacccgccag tgctcgcgct ggctcgaggc
     3961 gcgcaccggt gcctccaagg ccttgctgac ccattcgtgc accgccgcgc tcgagatggc
     4021 ggcgatcctc gccgacctgg agccgggcga cgaggtcatc atgccggcct acaccttcgt
     4081 gtccaccgcg aatgccttcg tgttgcgggg cgcggtgccg gtgttcgtcg acgtccgccc
     4141 ggacacgctc aacatcgacg agcgtctgat cgaggcggcg atcaccgcgc gtacgcgcgc
     4201 gatcgtgccg gtgcactacg cgggcgtcgc ttgcgagatg gacacgatca tggacatcgc
     4261 ccgccgccac gacctgctcg tcgtcgagga tgccgcgcag gcggtgatgg cgagctacaa
     4321 gggccggccg ctcggcgcga tcgggcacat gggggcgctc tccttccacg agaccaagaa
     4381 catcatctcg ggcgagggcg gcgccctgct ggtcaatgct ccggggcgcg ccgagcgcgc
     4441 ggagatcatc cgcgagaagg gtacgaaccg ctcccagttc ttccgtggcc aggtggataa
     4501 atacacctgg gtggacatcg gctcgtccta cctccccggg gagctcatcg ccgcctttct
     4561 gtgggcgcag atggaggagg ccgacagcat cacgcgtcgc cgcctgggca tctggaacac
     4621 ctaccaccag tggttcgccg aggccgagcg ccagggccgg ctgcggcgtc cggtgattcc
     4681 ggcgcattgt gcgcacaatg cgcacatgta ttacctgctg ctgcccgggc tggagcagcg
     4741 taccgccttc atcgagcgcc tgcgcggcct cgggatccat tgcgtcttcc actacatccc
     4801 gctgcacgat gcgccggcgg gtcgccgctt cggccgtgcc cacggcgaac tgagcgtcac
     4861 cgaggacatc gcggaccgcc tggtgaggct acccctgtgg ctgggcatgg aggacgcgca
     4921 ggccgagatc atccagcaag tgctcgccgc gctctgagcg ccgcctgcca ccggcgcgac
     4981 gcatccgcat gagccgccat cgagcttgcc tgccgggcgc gggcgggggc tgcgcgtcgg
     5041 gtggggggcg cggccgggtg gcgggcagga cccgcgtact ctggctagcg gtcgggctcg
     5101 cgatgctgct cgtcgcgctg tcgcgcgcgc cgctcgcgga cgtgcattgg gatgcgccga
     5161 tctacctcta tcaggccaag cgcttcgccg agaccgccta cctcgtcgat ttcgcacgca
     5221 acgcgcagga gatcgccgcc cagctcgccg gtgcctggcc cgaggacgag agctactcgg
     5281 aggctttctg gcgatcgagc cgtctcggcc acatcgcgct gctcggcgcg ccggtggccg
     5341 cgttcggaag cacggacgcg gcgatcacgg ccgcccactg gatgttcgtc ctcgccatgc
     5401 cgctcggcct gctggcctgg tgccgggtga gccatctcgc cggacgagcg ctcgagccgg
     5461 gtgcggtcgg ggccggtggg ctggcgtcga gcgcccttct gttcgtgctc tccgacgttc
     5521 atggctacct gagcggcaac ctggtcagcg aggtgctcag cttcgggctc ggcgctgcgg
     5581 cggtgtgggc cttgctggcg tcgaccgcca cggccgcggg ctggctggcg gcggtctccg
     5641 gagggttggc cttcctcggc tacaccgtgc gggtggagag cgtgtggatc tggctggcgt
     5701 tcatgctcgc ctatctgcct tttgccggcg ccgcggaggc acgcgcacgg acgctgcgcc
     5761 gcttcgtcct cgcagggggg acggccctgc tcggctacgc cacctatgcg ttcgtcttcc
     5821 acccgctggc ggacccgcgc agctatctgg ccttcgccgc nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn gcggcgggct ggacctttgt gctgggggcg cggcagaggt
     6001 gcgcggccct cggcctggcg gtggttttcg tcctgatgtc gagacccccg atctatggct
     6061 ggttcgtcga gcaaccgggt ctctggcgga tccagcgcct cggtgcggcg atgttcgtgc
     6121 cgcgctacga gcgcgtcgat tacgtccccc gggagatggc ggcgatcagc gcgcaggtgt
     6181 tcggcggaga ggggcggccg ggaatcctga tcgcatcccc gtccgtcaaa caggaatacc
     6241 tcaacctggt gcgcttctac gggccggcct atccggccga tgcgcacctg gcactggcgg
     6301 gcgatcccac caaccccggg cgctgcgagc ttcgccgcga ccgcgcccac gagccggtac
     6361 gcttctgcac cggcttcgga gatgccgcgg ccttgcgcgc cagcctggtc gggcatcgcg
     6421 tgctccatct gcggcgggtc ggcgatccgc ccctggccgg tgccgcgccg ctgctgcaga
     6481 ccaccggctt cgtgctcgag caactcgggc cgctgcccgt tcccgatcca tctcccgagg
     6541 gctccccgcc atgagatcct ggcttccctg tgcgctcctc ttcgccctcg ccgcgctctt
     6601 tctcgcgccc gctctgggtg ggagcggatt ctggtggacc gacgagacgc ggcatgcgat
     6661 gggcggggtg ttcatcctcg acctgctgcg cgacctgccg ctcgacgacc cgatgggcta
     6721 cgccctgcgc tatttcgcgc agtatcctgc gctcgcgctt aactggtacc tgcccggctt
     6781 ctatttcgtc gaggctggtt tcttcgcgct cttcggggtc agcgagacgg ttgcgcactg
     6841 gaccgtgttc gccttcgtcc tgctcgccgc cgggctgtgg ttcgactggg tgcgcacggg
     6901 ctggaacccc ctgatggcct tcatggcgac ggcactgttc atcgcggtgc cggagtggaa
     6961 ccattgggcg cgcagcgtca tgctcgaggc gccggcgatc gccttgctcg tcctcgtggt
     7021 gtggaccttc gagcgctatc tcgaccgccc ggcatggtcg cgggccatcg cggccgggtt
     7081 ggcgatcgcc gccgcactgg cggtcaagca gaccgtcgcg ctcctgctgc cggcgctgct
     7141 ggcgtacggc ctgatctcgt cgcggcgtgc agccctgtgg cgcgtgcagg ccgtgcccgc
     7201 ctatctgatc gcggcgctgg cgatcgccct ggtcgcgctg catgcgctca agttcggcaa
     7261 tctcggcctc gcggccaccg tcggggacag ccgggtcgaa gtcggacaaa gtgcggcccg
     7321 cctctcgctc gaacgctggc tgacctatcc gcgagccctg atcgaggtct ggggctggcc
     7381 gctggcgctg ctgtcgctcg cgggggcgct gcttccggca cgccagggcg agggccggct
     7441 ggccctgatc cacgcctggc tgctgtgctg gtacgtggtg atgacgctgg tgctcggcgg
     7501 tgcgggcaat gcgccgcgct acacgctgta tgccctgcct gcgctcgccg tgctggccgc
     7561 ccgtccgctg ttcctgctgc gggggcggcg atcgcacgac ggcttcctcg tcctgctcgt
     7621 ggtcatgctc gctttccagg gctggcgaac gctgacgcag ccggtcccgc ggatcgacgg
     7681 ctatcgcgag gtggcgcagg cggtccacga gcggcggaca ggcgctcccg tgctgtttgc
     7741 cggcaagcat gacggcagct tcatcttcca tctgcggcag ctcgaccatg cgcgcgaggc
     7801 cgtggtgttg cgcgcggaca aggtcctggt ctcgctcgcg gtacacaagt acttcggcat
     7861 gagctcgcac gtctccggca tcgacgacat ccgcgccctg attcgacgct acggaatcga
     7921 atacgtggtg atcgagcgcc cggacatcgt cggcgttccc gagttcgaga tgcttgccga
     7981 gctgctcgcg caagccgatt tcgaacgcgt ggccgagttt cccgtgcgtg ccgccggcgg
     8041 ggccgaggca ccggagcgga tcgtcctcca tcgctaccgc gatcatgttc ccgcagccga
     8101 tgcggagatc gtgatcccgc taccgcatct cgggcgcgag atccgcttcc ggcgcgcggg
     8161 cggctgaggt cgctgcgggc gtcggcggca ggtccaggcg gggatctccg cgcagcgccg
     8221 ggtcgttcgc catccaggcg cgcagctgcg cccacaggcg ctcgacctct gcgggatgcc
     8281 gggacgcgac gtccgcggtg cacccggggt cgttctcgac gtcgtacagg ctgagaagcc
     8341 tgccgtgctc gagcggctgg tagacgagct tccagcgccc gtcccgcacc atgcggtcct
     8401 tggcgaccag taccgtctgg cggtagcgct cgcgcagcga caggctgccc gccgcgatgt
     8461 ccggaacgtc gagcagttcc agcaggttgg gatagctcag gtgtccttcg ggtagaccgg
     8521 ggaccggtgc gatccagatc ccggtctcgt tgaacgcgcg caggtgcagg tcggtcgccg
     8581 gatcggcgat cgccgggcgc agcgacaccc cgtcgcagcc gaccgagggc gcaccgagca
     8641 gatcgagcag ggtcggcatg atgtccacgc tgcgcaccac ctggtcgacg cgctggccgc
     8701 ccggtcgggc cgggtcgacg acgatcagcg gcacgcgcgc gctgaagtcg cccagggcgg
     8761 agttgccctg gccccaggtg ccgtgctcga agaactccat gccgtggtcg ctgtagagca
     8821 ccacgatggt gtcctccgcg aggccgctgt cgtcgagctg gcgcagcagg cgcccgacct
     8881 cgtcgtcgaa ctgggccacg cagccatcgt agagatcgag gatctggtcg aggtcgaact
     8941 cctcgcgcgg ctcgccctgg cggcggatga tctcgaacgg ttcggtcagc cgggccatgg
     9001 cgaacttgga cgcgccggaa taggcaggat cggagaaccg ggtgtagtag gggtgctccg
     9061 atgcgaaagg cggatgcgtg gtcgaataga agaggttgag caggaagggt tcgtcacccg
     9121 cagccaaacg cgacagcgtg cgtcgcgcgc ggcgtccgag catgccggtc tggggcacgc
     9181 cgccgaggta gtgaatctcg ggaagaaggt gacggccgag acgattgtgc aggaacagcg
     9241 acaggaacag gcggatgtcc ttcgggccct ggcggatgag gtacttcagg ttccactgat
     9301 cctcgggcag atcggtgatg tcgaagccga aatcgaactt gcccagatcg gcgccgcacc
     9361 agtcggaaac cgccgcggtg cgatagccca gtgcgcgcag tatgttcggc agggtcttgt
     9421 cttcgagccg cgtctcggcg ccggccacgt agttgtcgcg caccccgtgg tggtgcggcc
     9481 aggtcgcggt gaacagcgag atcaggctcg gtgcggtgcg cgcgcagggg acgtagcagg
     9541 cgccgaagcg ggtgcctcgt gcggccagcg cgtcgatgtt cggggtgatc gacgcgcctt
     9601 cgcgttgcgc gccgatgcgg tcggcccgga gcgtatcgct gccgatcatc agcacgttgc
     9661 gccgcgcgcg ccgtcgcggg gtgggggcgt gtgtgccgtc gggctcccag atcgcccagg
     9721 cgagcagcgc gatgtagccg caggtaacga gcagcatggc ccccgcgtcc gcgctgccgc
     9781 cctggtggta gcgcagcaac gcgatcgccc cgaccaggcc gattccggtc gccagcacga
     9841 tgcgcgcgat gaggtcgaga ccgcgctgcg acagcgcgcg ccacaggcga tggatgcgga
     9901 cggtgcgata ttgccaggac gcgatcacca ggcccgggtt ttgcgaaagt gctcggcagc
     9961 cggcatacac cgtcgcgagc gcgagggtga gggcgccgcc cgcggcagtc gtccacagcg
    10021 gcggttcccg accgagcgct tccgccgcag tgatccacag cgcggtgccc gggaaggccc
    10081 agaacgcggc gaggccgagg gccgcgagcc ccaggcgggc gatggcgaag acgacgtagc
    10141 cgcgatagcg cgcgaggatc tcttcgcgca ggcccggccc ggtgcgcgag aagtcccgca
    10201 ccgacttggc taccagcacc gcgctcgtca gcgcgcccac cacgccggcg ccgagcaggg
    10261 cttcgcccag ccagtggcgc gcgagcaccg ccgccagcgc gagcgtgagc gcggcggcgg
    10321 ccagcaaggc cgccgatccc gcttccgccc cgagcatcgg cagtgccgca cgcctgcgct
    10381 tgcgccggtg gcggtcgcgg atgccggaga tctcgcccat cgaggaagcc aggcggacca
    10441 gatagaagcg ccgtgcctgc agccgccacg agaacgccag cccgagggcg tagcccgcgg
    10501 ccttgcgcca catgtagcgc ggcacccggt cgaactccgc gcgcacccgc gtgaccgatt
    10561 gcgagcgctg gtagcccttg cgtgcgacgt aggacaaggt caggcgttcg ctatcgacat
    10621 agtggtactg gaggatctgc ggtgcgtaat gcaggcgggc ccccttgcgc aaggcgcgca
    10681 ggatccagtc ggcgtcctcc gatccgccga gatcgtgtcc ggtcggcccg agttcgatcg
    10741 cgaacggtcc cgtcgcaccg atcaccggca ggcgggcgac gagattgccg cctcccggga
    10801 tcggcccctc gaggtcgatc gggaagtcct ccatgccctg gtcgtagcgc gggactggca
    10861 gcgggtagat gcgatagggg ccttcgtcgt gcacccaggc cggctcgctg ccgtcccagt
    10921 cgggcacgat ccgcccgcag aagagatcgg cctcgggcca gcgctccgcg gcggcggtga
    10981 ccccgagcag gtagtcggcg tccacgcggt ggtcgtcgtc gacgaaggcg accagctcgt
    11041 cctccagcaa gggcagggcg cggttgagcg catgcgactt tcccggcgtg ggctccgcga
    11101 tccacgacag cgccagccga tccgcgcggt cgctgcgccc ggccaggaac tcgtgcgtgc
    11161 cgtcggtgca ggcgttggcc acgacgaaca ggcggacact ccagccggca ggctggcggg
    11221 cggactcgag cgaatcgatg acgcgtgcga gcaggtccgc gcggttgtgc gtgcagatca
    11281 ggatggtgag gctgcgggcg gcggtggcga cgttcatagc cgtcctccgg cgagcacggc
    11341 gagggcgatc gaggcgatca gccgcccgcg cccgacgggc gcgcacgcga aggcgcgcgc
    11401 gagatcggcg agcgcagcgc cgcgatcgcc ctcgcggcag gcccacttgg cgtagtcgca
    11461 cagcgtgccc gcgtacagtg cgcgccagta ggcgccctgc ttgtcggcgg gtagcaggtg
    11521 acggttcttg cgcgtcatcg ccag
//
LOCUS       DAOQNN010000051        11541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2541_length_11541_cov_10.686923, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000051 DAOQNN010000000
VERSION     DAOQNN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2541_length_11541_cov_10.686923"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="PKV97_04940"
     CDS             <1..1593
                     /locus_tag="PKV97_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB05257.1"
                     /translation="ADAYDDWHGASGLELYRAAAAHAAAHLACTRQALSAEALNPLQM
                     ALIAVVEDARVEQLAIRKFPGLGPLWRKLHGATPDRCASIGDWLDRIARALLDPEYID
                     AAPLIAKARLLFATRSDHLGNNALSWEIGVALAHDLSHIGLTYHPRRDVQSAPYRDDN
                     RFIWEFDEVADFAAAVPWQEQQVVRHVDLMEFINEVDVETAGDDAQEVWILGSELYDD
                     DGTTWNAREGREPAPEPHHYPEWDYQIQLERPNWCTLLEKRVAPSDPADIEAIVTRHK
                     PLVSRLKFLIEALQPQGMQRLRKQEDGDEIDLNAAVRAMIDIKSGQMPDPRINIRHVR
                     KVRDIAVLLLIDLSESTNDAVAGSDSTVLQLAREATALLADAMNRIGDPFALHGFASD
                     GRHEIEYFRFKDFDEDYGELPRGRLAAMRSGKSTRMGTAIRHATRLLGRCPQQKKLLL
                     VLTDGEPADIDVRDPQYLRLDAKKAIDDAARGGVSTFCLNLDPQADQYVSRIFGANRY
                     LVLDHVERLPERLPLLYMGLTR"
     gene            1634..2719
                     /locus_tag="PKV97_04945"
     CDS             1634..2719
                     /locus_tag="PKV97_04945"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNB05258.1"
                     /translation="MSGHLKTLTRYLIETRQAHGAHSTGDLNGLLNAVTTAMKIIANQ
                     VNKGALVGALGNHSDDAINVQGEVQKKLDVMSNDAIISETEWVGHLAGMASEEMEDIM
                     PIPSKYKRGKYLLVFDPLDGSSNIDVNLTVGSIFSVLRAPEHKGAVTVDDFLQPGTQQ
                     VCAGFALYGPSTMLVLTTGHGVDGFTLDRDIGAFMLTHPQMRIPEDTKEFAINASNSR
                     FWEPPVSRYVEECLQGKTGPRGKDFNMRWIASMVAEAFRILTRGGVFMYPADNKDPGK
                     PGRLRLMYEANPIAFIIEQAGGAATTGRQRIMDVKPESLHQRIPLILGSRNEVERIER
                     YHREHEQGLDREFSSPLFNVRSLFSGQ"
     gene            2749..3630
                     /locus_tag="PKV97_04950"
     CDS             2749..3630
                     /locus_tag="PKV97_04950"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HNB05259.1"
                     /translation="MSAKHPVIAITGSSGAGTSTVTRTFQQIFRREHINAAIVEGDSF
                     HKYDRKSMKVAMAEAAKKGNNHFSHFGPESNLFEELETLFREYGASGSGKVRKYLHND
                     EEAAPYKQEPGTFTPWEDIEPGTDLLFYEGLHGAVKTDKVDIARHADLKIGVVPIINL
                     EWIQKLHRDKTQRGYTTEAVTETILRRMPDYVNFICSQFTHTHINFQRVPMVDTSNPF
                     IARSIPALDESMVVIRFSHPRGIDFPYLLSMIHDSFMSRPNTIVVPGGKMDLAMQLIF
                     TPMILRLVENARKHRAA"
     gene            3746..5770
                     /gene="tkt"
                     /locus_tag="PKV97_04955"
     CDS             3746..5770
                     /gene="tkt"
                     /locus_tag="PKV97_04955"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNB05260.1"
                     /translation="MSTFSDFEQPVFNNLTSAIRALAMDAVQKANSGHPGAPMGMAEI
                     AEVLWNHHLRHNPKNPKWADRDRFVLSNGHGSMLIYALLHLTGYDLSIDDLKQFRQLH
                     SRTPGHPEYGYTPGVETTTGPLGQGVTNAVGMALAEKMLAAEFNRDGFDIVDHYTYVF
                     LGDGCLMEGISHEACSLAGTWGLGKLICFYDDNGISIDGHVEGWFTDDTPKRFEAYGW
                     NVIPNVNGHDPEAIHAAINAAKAQGAKPTLICCKTVIGLGAPNKQGGHDVHGAPLGDA
                     EIAAARPHIGWNFGPFEIPQEVYEAWDGRRKGAELEAQWQRKFDQYAKHYPEQAAEFT
                     RRMSGELPAGWAEHARSVISKVNEKAETIATRKASQNCIEAYAPKLPELAGGSADLAG
                     SNLTLWSGSKAVSGKPGKEGGGNYIHFGVREFGMSAICNGVALHGGLIPYSATFLMFS
                     EYARNALRMAALMKIRQVFVYTHDSIGLGEDGPTHQPVEQTATLRMIPNMDVWRPCDT
                     VESAVAWASAIERKDGPSSLLFSRQNLAFQKRGAATIENIRRGGYVLSEAAGKPQAII
                     IATGSEVALAMSAQQTLAESGIHVRVVSMPSTNVFDRQDAAYRDSVLPRGVPRVAVEA
                     GVSDGWYKYVGLEGAVVGLDRFGESAPAGELFKAFGFTAANVAETVKRVL"
     gene            5877..6875
                     /gene="gap"
                     /locus_tag="PKV97_04960"
     CDS             5877..6875
                     /gene="gap"
                     /locus_tag="PKV97_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNB05261.1"
                     /translation="MAIKVGINGFGRIGRMVFRAAFRDFTDIEIVGINDLLEPDYLAY
                     MLQYDSVHGRFKGEVKVDGNTLVVNGKKIRLTAVKDPAELKWNEVGADVVIESTGLFL
                     TKETAEKHIAAGAKKVIMSAPSKDDTPMFVYGVNDKTYAGQAIISNASCTTNCLAPVA
                     KVLNDTFGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGK
                     VIPELNKKLTGMAFRVPTSDVSVVDLTVELNKEASYEQICAAMKAASEGPMKGVLAYT
                     EDKVVATDFRGESCTSVFDADAGIALDGTFVKVVAWYDNEWGYSCKVLEMARVISK"
     gene            6974..8140
                     /locus_tag="PKV97_04965"
     CDS             6974..8140
                     /locus_tag="PKV97_04965"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNB05262.1"
                     /translation="MNFLKMTDLDLAGKRVFIRADLNVPQDDAGNITDDTRIRASLPA
                     IERALKQGAAVMVTSHLGRPTEGEFKAEDSLAPIGTRMAELLGKPVRLVQNWVDGGFE
                     VKPGEVVLLENCRVNKGEKKSSDELSQKLAQLCDIWVHDAFGTAHRAEATTYGMGKYA
                     KVACAGPLVAGELDALGRALNQPARPLVAIVAGSKVSTKLTILASLADKVDQLVVGGG
                     IANTFLLASGKKIGKSLAEPDLVDEAKKVMAKTKVPLPVDVVCAKEFSLTAAATVKSI
                     DDVADDDMILDIGPQSAAQLAEVLKSAGTVVWNGPVGVFEFEAFAGGTKTVADAISSS
                     KAFSIAGGGDTIAAINKFGTKVSYISTAGGAFLEFLEGKTLPAVEILESRSRDR"
     gene            8222..9676
                     /gene="pyk"
                     /locus_tag="PKV97_04970"
     CDS             8222..9676
                     /gene="pyk"
                     /locus_tag="PKV97_04970"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNB05263.1"
                     /translation="MQRITKIVATLGPASSDPETLTRLIEAGVNVVRLNFSHGTADDH
                     RKRVETLRECAANLGKTVGIMADLQGPKIRVGRFAGSKVLLKNGQRFMLDTECPLGNS
                     ETVGLDYPELVNDVKAGDVLLLDDGRIVFDVESVGETEIACTVRHGGELSNNKGINRQ
                     GGGLSAPALTPKDMEDIRTAAQLNVDLVAVSFPKNGADMRQARELLRAAGSDALLIAK
                     IERAEAIPALEEIILSCDGIMVARGDLAVEVGDAAVPALQKRMIRMARELNRLTITAT
                     QMMESMISSPVPTRAEVSDVANAVLDGTDAVMLSAETASGSYPVQTVEAMARICLEAE
                     KSHEVTLDRDFLDRVFTRIDQSVAMAALFTAYHLKVKAIASLTQSGSTALWMSRLNCG
                     VPIYALTPEIRTRYKTALFRGVFPLMMLQYEDIDRDELLLQAERVLIDSGAVVKGDLI
                     LLTIGEPIGKSGGTNTLKIVRVGEQGEAPARTAN"
     gene            9707..10771
                     /gene="fba"
                     /locus_tag="PKV97_04975"
     CDS             9707..10771
                     /gene="fba"
                     /locus_tag="PKV97_04975"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988217.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HNB05264.1"
                     /translation="MPLVSMRQLLDHAAENGYGLPAFNVNNLEQVRAIMEAADETGSP
                     VIMQGSAGARKYAGEAFLRHLIQAAIEAYPHIPVVMHQDHGQSPAVCQGAIRSGFSSV
                     MMDGSLMEDGKTPSSYEYNVETSRRVVDFAHAIGVTVEAELGVLGSLETMKADKEDGH
                     GAEGTMTREDLLTDPDQAADFVQKTQCDALAIAIGTSHGAYKFTRKPTGDILAIDRIK
                     EIHARIPNTHLVMHGSSSVPQEWLEIIRKYGGQMKETYGVPVEEIVTGIKHGVRKVNI
                     DTDIRLAMTGAMRRHMAEKPSEFDPRKFLADAQKAAKEICKLRYEAFGCAGQAAKIKP
                     ISLEKMAERYKSGELNQIVK"
     gene            10879..11334
                     /locus_tag="PKV97_04980"
     CDS             10879..11334
                     /locus_tag="PKV97_04980"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNB05265.1"
                     /translation="MDINAKAFRHIQHFFRLGLDGVEYAGLLVIAFATSVAMYDEVMV
                     MIGRGKVTLTDLLLMFLYLEVLAMIGRYFSSGQLPVRFPLYIAMVALARYLILDLKEM
                     TEWRMLAVAAAIFLLTLGVLVIRYGHVKFPYKEDKETEVSDKAKVYVRE"
BASE COUNT         2320 a         3852 c         3516 g         1853 t
ORIGIN      
        1 gccgacgcct acgacgactg gcacggcgcg agcggcctcg agctttaccg cgccgccgcc
       61 gcacatgcgg cagcgcatct ggcctgcact cgccaggcgc tttccgcgga agcgctcaac
      121 ccgctccaga tggcgctgat cgccgtggtc gaggatgcgc gcgtcgagca actcgcgatc
      181 cggaagtttc ccgggctcgg cccgctatgg cgcaagctgc atggcgcgac acccgaccgt
      241 tgtgcgtcga tcggcgactg gctcgaccgc atcgcgcgtg cgctgctcga tcccgaatac
      301 atcgatgccg cgccactgat cgcgaaggcc cggctgttgt tcgcgacgcg cagcgaccat
      361 ctcggcaaca acgcgctgtc gtgggaaatc ggcgtcgcgc tcgcacacga cctgtcccat
      421 atcggcctga cttaccatcc tcgccgcgac gtgcagtctg cgccctaccg cgacgacaac
      481 cggttcatct gggaattcga cgaggttgcc gacttcgctg ccgcggtgcc ttggcaggaa
      541 caacaggtgg tccggcatgt cgacctgatg gagttcatca atgaggtcga cgtcgaaacg
      601 gccggcgacg acgcccagga agtctggatc ctcggcagcg aactctacga cgacgacggc
      661 acgacatgga atgcccgtga aggccgcgaa ccggcgcccg aacctcacca ctatccggag
      721 tgggactatc agatccagct cgagcgcccg aactggtgca ccctgcttga gaagcgcgtc
      781 gcgccgtccg acccggccga cattgaggcc atcgtcacgc gccacaaacc gctggtgtca
      841 cgcctgaagt tccttatcga ggcattgcag ccgcaaggca tgcagcgtct gcgcaaacag
      901 gaggacggcg acgagatcga tctcaatgcc gccgtgcgcg cgatgatcga catcaagagc
      961 ggccagatgc ccgatccgcg catcaacatc cgccacgtgc gcaaggtgcg cgacattgcg
     1021 gtgctgctgc tgatcgacct ttccgaatcg accaatgacg cggtggcagg cagtgacagc
     1081 accgtgctgc agcttgcgcg cgaagccacc gccctgctcg cggatgccat gaacaggatc
     1141 ggcgatccgt tcgccctgca tggctttgcc tcggatggcc gtcacgaaat cgagtacttc
     1201 cgcttcaagg atttcgacga ggattacggc gaactgccgc gcggacggct cgccgcgatg
     1261 cgaagcggca agtccacccg catgggtacg gcgatccggc acgcgacgcg cctgctcggg
     1321 cgttgtccgc agcagaagaa attgctgctg gtcttgaccg acggcgaacc ggcggacatc
     1381 gacgtgcgcg acccgcagta cctgcgactg gacgcaaaga aggcgatcga cgatgccgcg
     1441 cgtggcggcg tgtcgacctt ctgcctgaat ctcgatccgc aggccgacca gtatgtttcg
     1501 cgcattttcg gcgccaaccg ctatctggtt ctggatcacg tggagcggct gcccgaacgc
     1561 ctgccgctgc tctacatggg cctgacgcgc tgaaacacga gaccgacacc attactacaa
     1621 aagggagaac gacatgagtg gccacctcaa gaccctgacc cgctacctga tcgaaacacg
     1681 acaggcacac ggcgcacact cgaccggcga cctcaacggc ctgctcaatg ccgtcacgac
     1741 ggcgatgaag atcatcgcca accaggtcaa caagggtgct ctggtcggcg cgctcggcaa
     1801 tcacagcgac gacgcaatca acgttcaggg cgaggtgcag aagaagctcg acgtcatgag
     1861 caacgacgcg atcatcagcg agaccgaatg ggtcggacac cttgcgggca tggcgtccga
     1921 ggagatggaa gacatcatgc cgatcccgag caagtacaag cgcggcaagt acctgctggt
     1981 gttcgacccg ctcgatggat cctcgaacat cgacgtgaac ctcaccgtcg gttcgatctt
     2041 ttcggtgctg cgggcgccgg aacacaaggg agcggtgacg gtcgacgatt tcctgcaacc
     2101 cggcacgcag caggtgtgcg ccggcttcgc gctgtacggt ccctccacga tgctggtact
     2161 gaccaccggc cacggcgtcg acggcttcac gctcgaccgg gacatcggtg ccttcatgct
     2221 tacgcatccg cagatgcgca tccccgaaga cacgaaggaa ttcgcgatca acgcctcgaa
     2281 ttcgcgcttc tgggagccgc ccgtgagccg ctacgtcgag gaatgcctcc agggcaagac
     2341 cggcccgcgc ggcaaggatt tcaacatgcg ctggatcgcg tcgatggtcg ccgaggcgtt
     2401 ccgcatcctg acccgcggcg gcgtgttcat gtacccggcc gacaacaagg atccgggcaa
     2461 gccaggacga ttgcgcctga tgtacgaggc caacccgatc gccttcatca tcgaacaggc
     2521 gggtggcgca gcgaccaccg ggcgccagcg catcatggac gtcaagccgg aaagcctgca
     2581 ccagcgcatc ccgctgatcc tgggctcgag gaacgaggtc gagcgcatcg agcgctacca
     2641 ccgcgagcac gagcagggac tggatcgcga attcagctcg ccgctgttca acgtccgctc
     2701 gctgttctcc ggccaataag aaatttcacg catcgaagga accacaccat gtccgccaaa
     2761 catcccgtca ttgccataac tggctcttcc ggcgccggca ccagcaccgt cacccggacc
     2821 ttccagcaga tcttccgccg cgaacacatc aacgccgcga tcgtcgaggg cgactcgttc
     2881 cacaaatacg atcgcaagtc gatgaaggtc gccatggccg aggccgccaa gaagggcaac
     2941 aaccacttca gccacttcgg ccccgagtcg aacctgttcg aggaactgga aacgctgttc
     3001 cgcgagtacg gcgcaagcgg cagcggcaag gttcgcaagt acctgcacaa cgacgaagag
     3061 gccgcgccct acaagcagga gcccggcacc ttcacgccct gggaggacat cgaacccggc
     3121 accgacctgc tgttctacga aggcctgcac ggcgcggtga aaaccgacaa ggtcgacatc
     3181 gccaggcacg ccgacctcaa gatcggcgtg gtgccgatca tcaacctcga gtggatccag
     3241 aagctgcacc gcgacaagac ccagcgcggc tacacgaccg aagcggtcac cgaaaccatc
     3301 ctgcgtcgca tgcccgatta cgtgaacttc atctgctcgc agttcacgca cacacacatc
     3361 aacttccagc gcgtgcccat ggtggatacc tcgaacccgt tcatcgcgcg ttcgatcccg
     3421 gcgctcgacg aatccatggt cgtgatccgt ttctcccatc cgcgcggcat cgactttccc
     3481 tacctgctgt caatgatcca cgactccttc atgtcgcggc ccaataccat cgtggtgccg
     3541 ggcggcaaga tggatctcgc gatgcagttg atcttcacgc cgatgatcct tcgactggtc
     3601 gaaaacgcgc gcaagcaccg cgcagcctga cctcgcggcg ggcgcgccac gcatgcgccc
     3661 gccgcgtcca tcctgttcaa gggggccgga ttcggcgata atttcaggtt ttccgacctg
     3721 cctccccgcc ttctgggagc ctttgatgtc tacattcagc gatttcgagc agcccgtgtt
     3781 caacaacctt accagtgcaa tccgcgcgtt agccatggat gccgtgcaga aggcgaattc
     3841 ggggcacccc ggcgcgccga tgggcatggc cgaaatcgcg gaagtgcttt ggaatcatca
     3901 ccttaggcac aatccgaaga accccaagtg ggccgaccgc gaccgtttcg tgctgtccaa
     3961 cgggcacggc tcgatgctga tctacgccct gctgcacctc acgggctacg acctttcgat
     4021 cgacgatctc aagcagtttc gccagctcca cagcaggacg ccgggccacc ccgaatacgg
     4081 ctacacgccc ggcgtcgaga ccaccaccgg accgctcggc cagggcgtca ccaatgcggt
     4141 cggcatggcg ctggcagaaa agatgctcgc ggcggaattc aaccgcgacg gcttcgatat
     4201 cgtcgatcac tacacctacg tgttcctcgg cgacggctgc ctgatggaag gcatctcgca
     4261 cgaggcctgc tcgcttgccg gcacctgggg cctgggcaaa ctgatctgct tctatgacga
     4321 caacggcatc tcgatcgacg gccatgtcga aggctggttc accgacgaca cgccgaagcg
     4381 cttcgaagcc tacggctgga acgtgatccc gaacgtcaat ggtcacgatc ccgaggccat
     4441 ccatgccgcg atcaatgccg ccaaggcgca gggcgccaag ccgacgctga tctgctgcaa
     4501 gacggtgatc ggcctcggtg cgccgaacaa gcagggcggc cacgacgtcc atggcgcacc
     4561 gctcggcgat gccgagatcg ccgcggcccg tccgcacatc ggctggaatt tcggcccctt
     4621 cgagattccg caggaagtat acgaagcctg ggatggccgc cgcaagggcg ccgaactcga
     4681 ggcccaatgg cagcgcaagt tcgaccagta tgccaagcac tatcccgaac aagccgccga
     4741 gttcacgcgc cgcatgagcg gcgagctgcc cgccggctgg gccgagcatg ccaggtcggt
     4801 gatcagcaag gtcaacgaga aggccgaaac cattgccacg cgcaaggcaa gccagaactg
     4861 catcgaggcc tatgcgccga agctgcccga actcgccggc ggctcggccg acctcgccgg
     4921 ctccaacctg acgctgtggt cgggctcgaa ggctgtgagc ggcaagccgg gcaaggaagg
     4981 cggcggcaac tatatccact tcggcgtgcg cgaattcggc atgtcggcca tctgcaacgg
     5041 cgtcgcgctg cacggcggac tgatccccta ctcggccacc ttcctgatgt tctcggagta
     5101 cgcgcgcaac gcgctgcgca tggccgcgct gatgaagatc cgccaggttt tcgtctacac
     5161 ccacgattcg atcggcctcg gcgaggacgg cccgacgcac cagccggtgg aacagaccgc
     5221 cacgctgcgc atgatcccga acatggacgt ctggcgcccg tgcgatacgg ttgaatcggc
     5281 tgtcgcctgg gccagtgcaa tcgagcgcaa agatggtccg tccagcctgc tgttctcgcg
     5341 ccagaacctg gcgttccaga agcgcggcgc ggccaccatc gagaatatcc gtcgcggcgg
     5401 ttacgtgctg tccgaagccg ccggcaagcc gcaggcgatc atcatcgcga ccggctccga
     5461 agtggccctc gcaatgagtg cccagcagac gctggccgaa agcggcatcc acgtgcgcgt
     5521 cgtgtcgatg ccatcgacca atgtgttcga ccgccaggat gcagcctacc gggattcagt
     5581 actgccgcgt ggcgtgcctc gtgtggcggt cgaagccggc gtcagtgacg gctggtacaa
     5641 gtatgtcggc ctggaagggg ccgtggtcgg cctcgatcgc ttcggcgaat cggcaccggc
     5701 cggcgaactt ttcaaggctt tcggtttcac cgccgccaac gtggcggaga ccgtcaagcg
     5761 cgtgttgtaa cccgcatcgc tgcacgattc ggcgttgcgg cggggattgg gggctggctc
     5821 gcctgcgcgg accgcaacgg cgccagaggg agagtttttc ttgatcggag gttagcatgg
     5881 ccatcaaagt tggcatcaac ggattcggac gcatcggtcg catggtgttc cgcgcggcgt
     5941 tccgcgattt cacggacatc gaaatcgtcg gcatcaacga cctgcttgag ccggattacc
     6001 tcgcctacat gctgcagtac gactccgtgc atggccgctt caagggcgaa gtcaaggtgg
     6061 acggcaacac tttggtggtg aacggcaaga agatccgtct gaccgccgtg aaggacccgg
     6121 ccgagctgaa gtggaacgaa gtcggtgcgg acgtggtgat cgaatcgacc ggcctcttcc
     6181 tcaccaagga aaccgccgag aaacacatcg ccgccggtgc gaagaaggtc atcatgtcgg
     6241 ctccgtcgaa ggacgacacg ccgatgttcg tctacggcgt caacgacaag acctacgcag
     6301 gccaggcgat catctccaac gcttcgtgca ccacgaactg cctcgcaccg gtcgccaagg
     6361 tgctcaacga caccttcggc atcaagcgcg gcctgatgac gaccgtccat gccgcgacgg
     6421 ccacgcaaaa gaccgtcgac ggcccgtcca acaaggattg gcgcggtggc cgcggcatcc
     6481 tcgagaacat catcccgtcg tcgaccggcg cggccaaggc cgtcggcaag gtgatccccg
     6541 agctgaacaa gaaactgacc ggcatggctt tccgcgtgcc gacttccgac gtttcggtgg
     6601 tcgacctcac cgtcgaactc aacaaggaag ccagctacga gcagatctgc gccgcgatga
     6661 aggcagcctc ggaaggcccg atgaagggcg tcctcgcgta caccgaagac aaggtggtcg
     6721 ccaccgattt ccgcggcgag agctgcacct cggtgttcga cgccgatgcc ggcatcgcgc
     6781 tcgacggcac cttcgtcaag gtcgtcgcgt ggtacgacaa cgagtggggc tactcgtgca
     6841 aggtgctcga gatggcccgc gtcatctcga agtaatcggc gacgaagaac accgttcgta
     6901 tccggtgcgg cttcggccgc gccggcgcct gtccccgcag agccctcaat tcatcccgtt
     6961 tccggagctc agcatgaact ttctcaaaat gaccgacctc gacctggccg gcaagcgcgt
     7021 attcatccgt gccgatctga acgtgccgca ggacgacgcc ggcaacatca ccgacgacac
     7081 gcgcatccgt gcctcgctgc ccgcgatcga gcgcgcgctc aagcaaggcg ccgcagtcat
     7141 ggtgacctcc cacctcggcc gtccgaccga aggcgaattc aaggccgagg attctctcgc
     7201 gccgatcgga actcgcatgg ccgaactgct cggcaagccg gtgcgcctgg tgcagaactg
     7261 ggtcgacggg ggcttcgaag tgaagcccgg cgaagtcgtg ctgctggaaa actgccgcgt
     7321 caacaagggg gagaagaagt cctccgatga actgtcgcag aagctcgccc agctgtgcga
     7381 catctgggtg catgacgcct ttggcaccgc gcaccgcgcc gaggccacga cctacggcat
     7441 gggcaagtac gccaaggttg cctgcgccgg tccgctggtc gccggcgaac tcgacgccct
     7501 cggccgcgcg ctcaaccagc cggcacgtcc tctggtggcc atcgtcgccg gctccaaggt
     7561 gtcgaccaag ctgaccatcc ttgccagcct cgccgacaag gtggatcaac tggtggtcgg
     7621 cggcggcatc gccaatacct tcctgctcgc ctcgggcaaa aagatcggca agtcgcttgc
     7681 cgaacccgat ctcgtcgacg aggcgaagaa ggtcatggcc aagaccaagg tgccattgcc
     7741 ggtcgacgtg gtctgcgcga aggaattttc cttgaccgcc gccgcaaccg tgaagtcgat
     7801 tgacgacgtc gccgacgacg acatgatcct cgacatcggc ccgcaatccg ccgcacagct
     7861 cgccgaagtg ctcaaatccg ccggcacggt ggtctggaac ggcccggtcg gggtgttcga
     7921 gttcgaggcg ttcgccggtg gcacgaagac cgtcgccgac gcgatctcgt cgtcgaaggc
     7981 attctcgatc gccggtggcg gcgacaccat cgcagcaatc aacaagttcg gcacaaaggt
     8041 cagctacatc tcgaccgccg gcggcgcatt cctcgaattc ctcgaaggca agaccctgcc
     8101 tgccgtggag attctcgaat ccagaagtcg cgacaggtaa cacgcatcgc accgggtgtg
     8161 ccgctgtgag ggacggcaca ccccgattcc tgcagtacca ctcggaggtc gaagccggaa
     8221 catgcagcga ataacaaaaa tagttgccac actgggcccg gcgtcaagcg acccggaaac
     8281 tttgacccgt ctgatcgagg ccggcgtcaa tgtcgtgcgc ctgaacttct cgcacggcac
     8341 ggcggacgac caccgcaagc gtgtcgaaac cctgcgcgaa tgcgcagcga acctcggcaa
     8401 gaccgtcggc atcatggccg acctgcaggg gccgaagatc cgtgtcggca ggttcgccgg
     8461 aagcaaggtc ctgctgaaga acggccagcg cttcatgctc gacaccgaat gcccgttggg
     8521 taattccgag accgtcggac tcgactaccc ggaactggtc aacgacgtga aagccggcga
     8581 cgtgctgctg cttgacgacg gccgcatcgt gttcgatgtc gagagcgtgg gcgaaaccga
     8641 gatcgcctgc acggtgcgcc acggtggcga gctgtcgaac aacaagggca tcaatcgaca
     8701 gggcggcggc ctctcggcgc cggcgttgac ccccaaggac atggaagaca tccgcaccgc
     8761 ggcgcaactc aacgtcgacc tggtcgcagt gtccttcccg aagaacggcg cagacatgcg
     8821 tcaggcgcgc gaactgctgc gagctgccgg atcggacgcc ctgctgatcg ccaagatcga
     8881 gcgtgccgag gcaataccgg cgctcgaaga aatcattctc tcgtgcgacg gcatcatggt
     8941 ggcgcgcggc gacctcgcgg tggaagtcgg cgacgccgcc gtgccggcgc tgcagaagcg
     9001 catgatccgc atggcgcgcg agttgaaccg actcaccatc acggcgacgc agatgatgga
     9061 gtcgatgatc tccagcccgg tgccgacgcg tgccgaggta tcggacgtcg ccaacgcggt
     9121 gctcgacggc accgacgccg tgatgctgtc ggcggaaacc gcctcgggca gctatccagt
     9181 gcagaccgtc gaagccatgg cgcggatctg cctcgaagca gagaaatccc acgaagtcac
     9241 gctcgaccgc gacttcctcg accgcgtatt cacgcgcatc gaccagtcgg tcgcgatggc
     9301 agcgctattc acggcctatc acctgaaggt gaaggcaatc gcctcgctca cccagtcggg
     9361 atcgaccgca ctgtggatgt cgcgcctcaa ttgcggcgtg ccgatctacg ccctgacgcc
     9421 ggagattcgc acgcgctaca agaccgccct gttccgcggc gtgttcccgc tgatgatgct
     9481 gcagtacgaa gacatcgacc gcgacgagtt gctgctgcag gcagagcgcg tgctgatcga
     9541 cagcggcgcg gtggtcaagg gcgacctgat cctgctcacc atcggcgaac cgatcggcaa
     9601 gtccggcggc accaacaccc tgaagatcgt acgtgtcggc gagcaaggcg aggcgcctgc
     9661 ccgcacggcc aactgaccca cattcatcca cctcaaggag actgccatgc ccctcgtttc
     9721 gatgcgccag ttgctcgacc acgccgccga gaacggctac ggcctgccgg ctttcaacgt
     9781 gaacaacctc gaacaggttc gcgcgatcat ggaagcagcc gacgagaccg gcagcccggt
     9841 catcatgcag ggctcggccg gagcgcgcaa atacgccggc gaggccttcc tgcgccacct
     9901 gatccaggcc gcgatcgaag cctatccgca catcccggtc gtcatgcacc aggaccacgg
     9961 ccagtcgccc gcggtgtgcc agggggcgat ccgctccggc ttctccagcg tcatgatgga
    10021 cggctcgctg atggaagacg gcaagacccc gtcgtcctac gagtacaacg tcgagacctc
    10081 gcgccgggtg gtcgacttcg cccatgccat cggcgtgacg gtggaagccg aactcggcgt
    10141 gctcggctcg ctcgagacga tgaaggccga caaggaagac ggccacgggg ccgaaggcac
    10201 gatgacgcgc gaagacctgc tgaccgatcc cgaccaggcg gccgacttcg tgcagaagac
    10261 gcagtgcgac gcgctggcca tcgcaatcgg cacctcgcac ggcgcctaca agttcacccg
    10321 caagccgacg ggcgacatcc tcgccatcga ccgcatcaag gaaatccatg cccgcatccc
    10381 gaacacgcac ctggtgatgc acggctcctc gtcggtgccg caggagtggc tcgagatcat
    10441 ccgcaagtac ggcggccaga tgaaggagac ctacggcgtg ccggtcgagg agatcgtcac
    10501 cggcatcaag cacggcgtgc gcaaggtcaa catcgacacc gacatccgcc tcgcgatgac
    10561 cggcgcaatg cgccgtcaca tggccgagaa accgtcggaa ttcgatccgc gcaagttcct
    10621 cgccgacgcg cagaaggccg ccaaggaaat ctgcaagttg cgctacgaag ccttcggctg
    10681 tgccggccag gccgcgaaga tcaagccgat ctcgctcgag aaaatggcgg agcgctacaa
    10741 gtcgggcgaa ctcaaccaga tcgtcaagtg ataccctgaa gtccgcggag cgccccggcg
    10801 ctccgacgga cgcgcgggcg gcccggcgca agtcgtgccg cccgatgttt ttgcaagcct
    10861 gcaacggaac cgcggttcat ggacatcaac gccaaagcct tcagacacat ccagcatttc
    10921 ttccgcctcg gtctcgacgg cgtcgagtat gccggcctgc tggtcatcgc cttcgccacc
    10981 tcggtcgcca tgtacgacga agtcatggtg atgatcgggc gcggcaaggt caccctgacc
    11041 gacctgctgc tgatgttcct ctacctcgaa gtgctggcga tgatcggccg ctacttctcc
    11101 agcggccagt tgccggtgcg ctttccgctc tacatcgcga tggtcgcact cgcccgctac
    11161 ctgatcctcg acctcaagga aatgaccgag tggcgcatgc tggccgttgc cgcggcgatc
    11221 ttcctgctca cactgggcgt gctggtcatc cgctacgggc acgtcaaatt cccgtacaag
    11281 gaagacaagg aaaccgaggt cagcgacaag gccaaggtct atgtacgcga atgacgaatc
    11341 ctgcaacatg ccggcctgga aggcgcatgg ataggcggtc gcagacctgc catcctgtaa
    11401 acgaccaatc ggcgggcctt gcgcgacatg gccgccagag acgcccggcc catgcggcct
    11461 ccaggcctga ctgaccaacc gaaccaccat gccgatccaa tggtttcccg gccacatgac
    11521 gctcgcccgc aagcaggcgg c
//
LOCUS       DAOQNN010000052        11389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2606_length_11389_cov_9.311893, whole genome shotgun sequence.
ACCESSION   DAOQNN010000052 DAOQNN010000000
VERSION     DAOQNN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11389
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2606_length_11389_cov_9.311893"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..779)
                     /locus_tag="PKV97_04985"
     CDS             complement(171..779)
                     /locus_tag="PKV97_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB05266.1"
                     /translation="MIARILCSLLLFCIPFAAHAGAVFQERGDAGDFASPQDVVGTSI
                     ERIQGSIGGDDSIDVFRFHFAGGPLAILARLDGFDDEALPIRLVRESGLPDEACFVGS
                     AEYPPNPCISEGWLDFSVDMLAPGNYLLGVCASAQGCVADDPPFTIGFVTHPDASAPT
                     ATVGRPVPEPGVLALLGLALLGLWGLRMAIAAPPKGPPPSGG"
     gene            complement(939..2636)
                     /locus_tag="PKV97_04990"
     CDS             complement(939..2636)
                     /locus_tag="PKV97_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB05267.1"
                     /translation="MRFGIAARLGIVLALSGMLSAGITAFQAQRASETVLVDSARAEL
                     LTSTRVLAQRITQVREDISRTLLALARHPAALAALESDDPQAATQLATLLALPMAANR
                     AYFQLRLIGAADHGLERVRIDRDGDTLLRVDGDALQEKGHYPYVFDALALAAGEVYMS
                     RVSINRERGSHAALGRPAVQLATPVVDESGRPRGVIVGSIDLNGVFALLAADLPADFL
                     LYLANSAGDYLIHPDPARTFGFDKGRRVRVQDEFPATQAVIDGRSSESMIDSDAGEHA
                     QAPLVAAFIAARVQLRTEEGAFILGLARPRAAVLAELDPLRGIMLQIVIGMSLTGILL
                     AAILARAITHPIEVLGAAVQNFYDNGQAPALPVERQDEIGALARRFAELRTQIGQQLA
                     ELEHRRRELEHLARHDALTGLPNRALFDDRMAVALASARRDDGGLALLFVDIDDFKPI
                     NDRLGHVVGDRVLEGVAARIRQSIRESDTAARIGGDEFVVLLPGIASAEDARVVADKI
                     RAAIDRPFMIDGGELAVSVSMGLAHYPQDGSDSTTLARHADAAMYRDKAEGKARRHS"
     gene            complement(2633..3682)
                     /locus_tag="PKV97_04995"
     CDS             complement(2633..3682)
                     /locus_tag="PKV97_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNB05268.1"
                     /translation="MLRRSSLHRLPWLVLALALSGTACATEVLRVLSWPGYADADVVQ
                     AFEARTGARVEVTQVDSDERLWQKLSTNDATDYDVFAVNTAELQRYIDRGVAVAIDPA
                     ALPNLGAQLPRFRDPATLPGTTRDGKLYAIPYAWAEMGLIYDRRQFDAPPQSIAALWD
                     ARYRGKVLVYNSGSHNFSLAAQMLGKASPFRLDAADWAPAVERLVELRRNLLTFYAQP
                     EESAHLFVSRGAALMYANYGTQQLQLLRAAGADVGYAIPREGALAWLDCWVVTRGARN
                     QALALAWIDHLLGTGPAHVLSARHGLDNTRDPAPHQAETDRLVWLEPVEDVERRNLLW
                     ERILSGDRGARVLAP"
     gene            3932..4954
                     /locus_tag="PKV97_05000"
     CDS             3932..4954
                     /locus_tag="PKV97_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB05269.1"
                     /translation="MDPIISISALSKTYASGFSALKGVDLDIRRGEIFALLGPNGAGK
                     TTLISIVCGLVNASAGSVRVDGHDIVRDYRAARALVGLVPQELTTDAFETVWNTVSFT
                     RGLFGKPPDPAYIEQLLKDLSLWAKKDARLMMLSGGMKRRVLIAKALSHEPRILFLDE
                     PTAGVDVELRRDMWQLVRRLRDSGVTVILTTHYIEEAEEMADRIGVIAKGALILVEDK
                     AMLMTKLGGKQLTLQLAAPLAEVPPALARYGLVLGKGGKTLVYRYEVGRGEGGEGGEG
                     SDPAGDGAIAGLLQDLAAAGIMFTDLHTTQRSLEEIFVSLVSEREAPGTDERGNRAEA
                     AGEGAR"
     gene            4951..5712
                     /locus_tag="PKV97_05005"
     CDS             4951..5712
                     /locus_tag="PKV97_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB05270.1"
                     /translation="MNWHAVRAIYLFEMARTKRTLLQSVLAPVITTSLYFVVFGSAIG
                     ARIPEIEGVSYGAFIVPGLIMLNLLTQSVSNASFGIFFPKFSRTIYEILSAPVSHFEI
                     VLSYVGAAATKAVILSLIILATATFFVPLRIEHPLWMIAFLLLTAVSFSLLGFILGIW
                     ADNFEKLQLVPLLIITPLTFLGGSFYSIDMLPAGWRTVSLFNPVVYLVSGFRWSFYGS
                     ADVAVGVSLAMTLLFLAVFVGVATWMLRTGYRLKP"
     gene            complement(5967..7277)
                     /locus_tag="PKV97_05010"
     CDS             complement(5967..7277)
                     /locus_tag="PKV97_05010"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNB05271.1"
                     /translation="MSATPQDKAALDRVGGLSYLNPAAPEPWASFVEQIERVRPHLGS
                     LERWIETLKHPKRALIVDVPIERDDGTVAHYEGYRVQHNLSRGPGKGGVRFHPDVTLN
                     EVMALAGWMTIKNAAVGLPFGGAKGGIRVDPASVSKGELQRITRRYTSEIGIIIGPDK
                     DIPAPDVGTNAMTMAIMMDTFSMNRGGTATGVVTGKPIALGGSLGRQEATGRGVFIAA
                     REAARHLRLPIEGARVVVQGFGNVGGIGARMFHDAGARVIAIADHTAILVNEAGIDIP
                     AALEHTAANGGLKGFAGAAPIDPEAFWRLECEFLVPAALEGQLTVERAAGARARIVVE
                     GANGPTTPAADDILHERGILVVPDVLANAGGVTVSYFEWVQDFSSFFWTEDEINERLE
                     RIMVAAFTAIWKVAQEKQVSLRTAAFIIACARVLEARAERGLYP"
     assembly_gap    7450..7549
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7550..8450)
                     /locus_tag="PKV97_05015"
     CDS             complement(<7550..8450)
                     /locus_tag="PKV97_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB05272.1"
                     /translation="MELKWLEDFLSLADSGSFSRSAEQRHVSQPAFSRRIRALEAWLG
                     VALVDRSTFPTRLTEAGEAFKGPAADLIHRLHAARALVRGHQTTAGDALVFAVPHTLA
                     FAFFPTWLGRLKAEFGEVPTRLIAGNVHDVVTMLVEGGCDLLLCYHHPTHPVWLDPAR
                     FEGIQLAVECVRPYLPRRLARSAGWQLPGSAEAPIPFLRYGPNTYLRRMVDTILDRAA
                     HPAHLALQYESDMAESLKAMLLAGHGLAFLPASAVAREVERGEVVVAGGPEWSLEMEV
                     RLYRDRANTKPELLRLWQHLAAKP"
     gene            8763..>11389
                     /locus_tag="PKV97_05020"
     CDS             8763..>11389
                     /locus_tag="PKV97_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB05273.1"
                     /translation="MIRTSLNFRLWLTIAIAVLPVLLFVFFDFHERREQAVAETRAEI
                     ALRLADANREAQAAHRAVALVLRIMARSNDLQALDSAQCSGIAGRLLESLDGFANIGA
                     ALPDGEIFCSGRPMSAPVTVIDRAWFRTSLDSDEISPGEFVIGRVSGTPGMAFGYPLR
                     AADGTLRAVIFATITVAWFDRLIEGFRLPEGWEASVISNSGLVLAHQPERAPEGWRAH
                     KVPATTLARMAAVVRDEGGIAELAGIDGVQRLYGIGVPDFAPGSGFFAIGAPLERSLE
                     AAGQRLLMYLALVAAIALASALIARLYIYRLIEVWATRVRGAIAEIAAGRRDARIGQA
                     TGVAELDALSEGIDQMALEIGKREGELQRLSMVIEQSPESIVITDTAGCILYVNEAFQ
                     RITGYARGEVLGRNPRILNGGLTPRATYEQMWATLLAGEVWRGEFHNVRKDGSAYLEL
                     ATIAPIKDAGGTVTHYVAVKEDITQRKQSEALLHRLAYFDALTGLPNRALLHDRIAQA
                     IRSGVRRESFGMLMLVDIDRFRQLNDTLGHAAGDRLLCEVAARLRASVREEDTVARHG
                     DDDFAVLVERIGETEADALSHAEYVARKLQRTLQQPYVLGGTEGDRHFATLSFGISLF
                     HDGVSSLESLLKQAEVALYRAKQDGRDIVRFFNPEMQAVVDAHARLEARMHEALEANA
                     FRLFFQPQVNRRGVVIGAEALLRWPLDGGAMVSPAEFIPLAEDTGHIVRLGSWVLRSA
                     CAQLARWQMNETTRHLKIAVNVSARQFHQPDFVASVKDTVRAAGVDPCRLELELTESA
                     ILSDMDETIRRMNELRALGIRFALDDFGTGYSSLSYLKRLPLDQLKIDQSFVRDMAED
                     EGSEAIVLAILSLSHALGL"
BASE COUNT         1626 a         3772 c         4128 g         1763 t
ORIGIN      
        1 ccgacgcgcg ggtgttctcg tccgggcgtt gggacctgcg cgccgacaac gccgaactgc
       61 atacgctcca ccacctcggc gccggcaact tcgcccgcgc cgccgacgtc gtccgcgcac
      121 gccctctgta ggcgcgccgg caggcgcgat cggccggacg aaaccggtct ttacccgccg
      181 gaagggggcg gtcccttggg cggtgcggcg atcgccatgc gcagtcccca caggcccagc
      241 aaggcgaggc cgaggagcgc gagcacgccg ggctccggca cgggcctgcc gacggtggcc
      301 gtaggtgcgc tcgcatcggg gtgggtcacg aagccgatcg tgaagggcgg atcgtccgcc
      361 acgcagcctt gtgcggacgc acaaacaccc agcaggtagt tgcccggcgc cagcatgtcg
      421 acggagaagt ccagccagcc ctcggagatg cacgggttgg gcggatactc ggcgctgccc
      481 acaaagcagg cctcgtcggg caggccgctc tctcgcacca ggcggatcgg cagggcttcg
      541 tcgtcgaagc cgtcgaggcg ggcgaggatc gcaagcgggc cgccggcgaa gtggaagcgg
      601 aacacgtcga tgctgtcgtc gccgccgatg ctgccctgga tgcgttcgat ggaggttccg
      661 accacgtcct gcggcgaggc gaaatcaccg gcgtcgccgc gttcctggaa caccgcgccg
      721 gcgtgcgcgg cgaacgggat gcagaacaac agcaggctgc agaggatgcg cgcgatcatg
      781 ctggcctcct tgctccggag tgtcgggtac tgccctccct tggggggaag cattcgccgt
      841 gcccgacgat cagaccattg attatctgcg cttatttttg aaggttcggc accgggtgta
      901 aaaatttccg acacttccat gccggctggc ggccggtttc agctgtgccg ccgtgccttg
      961 ccttcggcct tgtcgcggta catcgcggca tcggcgtggc gggcgagggt ggtggagtcg
     1021 ctgccgtcct gcgggtagtg ggcgaggccc atgctcaccg aaaccgcgag ctctccaccg
     1081 tcgatcatga acgggcggtc gatcgccgcg cggatcttgt ccgcgaccac tctggcgtct
     1141 tcggccgacg cgatcccggg cagcagcacg acgaactcgt cgccgccgat gcgcgccgcg
     1201 gtgtcggact cgcggatgct ctggcggatg cgcgccgcca cgccttcgag cacgcggtcg
     1261 ccgacgacgt ggccgaggcg gtcgttgatc ggcttgaaat catcgatatc gacgaacagc
     1321 aaggccagcc cgccgtcgtc gcggcgggcg gacgcgagcg ccaccgccat gcggtcgtcg
     1381 aagagcgcgc ggttgggcag cccggtgagc gcgtcgtggc gggcgaggtg ctcgagctcg
     1441 cggcggcggt gttcgagctc ggccaactgc tgcccgatct gggtgcgcag ctcggcgaag
     1501 cggcgggcga gggcgccgat ctcgtcctga cgctcgaccg gcagcgccgg ggcctggccg
     1561 ttgtcgtaga agttctgcac cgcggcaccc agcacctcga tcggatgggt gatcgcgcgg
     1621 gcgaggatgg ccgcgagcag gatgccggtg aggctcatgc cgatgacgat ctgcagcatg
     1681 atcccgcgca gcggatcgag ctcggcgagc acggcggcgc gtgggcgcgc gaggccgagg
     1741 atgaaggcgc cctcttcggt gcgcagctgc acccgcgcgg cgatgaaggc ggcgaccagc
     1801 ggcgcctgcg cgtgctcgcc ggcatcgctg tcgatcatcg actcgctgct gcggccgtcg
     1861 atcacggcct gcgtggcggg gaactcgtcc tgcacccgta cccggcgtcc cttgtcgaag
     1921 ccgaaggtgc gcgcggggtc ggggtggatc aggtagtcgc ccgcgctgtt ggcgaggtag
     1981 agcaggaagt cggcgggcag gtcggcggcg agcagggcga acacgccgtt gaggtcgatg
     2041 ctgcccacga tcaccccgcg tggccggccc gattcgtcga cgaccggggt ggcgagctgc
     2101 accgcgggcc ggccgagggc ggcgtgcgag ccacgctcgc gattgatgct cacgcgcgac
     2161 atgtaaacct cgcccgcggc cagcgcgagg gcgtcgaaga cgtaggggta gtggcccttt
     2221 tcctgcagcg cgtcgccgtc cacgcgcagc agcgtgtcgc cgtcgcggtc gatccgcacg
     2281 cgctcgaggc cgtggtcggc ggcgccgatc aggcggagct ggaagtaggc gcggttggcc
     2341 gccatcggca gggcgagcag ggtcgcgagc tgcgtcgcag cctgcgggtc gtcgctctcc
     2401 agcgccgcga gcgcggccgg gtggcgggcg agcgcgagca gggtgcgcga gatgtcctcg
     2461 cgcacctgcg tgatgcgctg cgcgagcacc cgggtggagg tgagcagctc ggcacgcgcg
     2521 ctgtccacca gcacggtctc gctggcgcgc tgggcctgga aggcggtgat gccggcagag
     2581 agcatgccgg acagggcgag tacgatgcca agacgggcgg cgatgccgaa tctcatggcg
     2641 cgagcacccg tgcgccgcgg tcgccggaga ggatgcgctc ccacagcagg ttgcggcgtt
     2701 cgacgtcctc gaccggttcg agccagacca ggcggtcggt ttcggcctgg tgcggcgccg
     2761 ggtcgcgggt gttgtcgagg ccgtggcgcg cgctcagcac gtgcgccggg ccggtgccga
     2821 gcaggtggtc gatccacgcc agcgcgagcg cctggttacg tgcgccgcgc gtcaccaccc
     2881 agcagtcgag ccaggcgagc gcgccttcgc gcgggatcgc atagcccacg tccgcccccg
     2941 ctgcgcgcag gagctgcagc tgctgggtgc cgtaattggc gtacatcagc gccgcgccgc
     3001 ggctgacgaa cagatgcgcg gattcttccg gctgggcgta gaaggtcagc aggttgcggc
     3061 gcaactcgac cagacgctcg accgccggcg cccagtcggc ggcgtcgagg cggaagggcg
     3121 atgccttgcc cagcatctgt gcggcgagcg agaagttgtg cgagccgctg ttgtacacca
     3181 gcaccttgcc gcggtagcgg gcgtcccaca gcgcggcgat ggactggggt ggcgcgtcga
     3241 actggcgccg atcgtagatc aggcccattt cggcccaggc ataggggatg gcgtacagct
     3301 tgccatcgcg agtcgtgccg ggcagcgtgg ccgggtcgcg gaagcgcggc aattgcgcac
     3361 cgaggttggg tagcgcggcc ggatcgatcg ccacggccac gccgcggtcg atgtagcgct
     3421 gcagctcggc ggtattgacc gcgaacacgt cgtagtcggt cgcgtcgttc gtgctgagct
     3481 tctgccacag cctttcgtcg gaatcgacct gggtgacttc gacccgggcg ccggtgcggg
     3541 cctcgaaggc ctgcaccacg tcggcgtcgg catagcccgg ccagctcagc acgcgcaaca
     3601 cctcggtcgc gcaggccgtg ccggagagtg cgagcgcgag cacaagccag gggaggcgat
     3661 gcaggctcga gcgacgaagc atggcggatc ggctcctgga tctggcggcg gggaaggatc
     3721 ttggggggtg cgaggcagtt taagcgcgcc gagcccgccg cggaagcgcg cccgacggct
     3781 ggccgccagc cgccgccggg cggtggacgg gccgcccatg ctggagcgcc gcaagtcgcg
     3841 atcgcggcct tggggcactc cgctgccgct catcgtgccc ggtgccgctc gatcctgcgg
     3901 gccgcctcgt cgatctgtta tcttcgatcc catggacccc atcatctcga tctccgctct
     3961 gtccaagacc tacgcctccg gcttctccgc gctgaagggt gtcgacctcg acatccgccg
     4021 cggcgagatc ttcgcgctgc tcgggcccaa cggcgcgggc aagaccacgc tgatcagcat
     4081 cgtgtgcggc ctggtcaatg ccagcgcggg cagcgtgcgg gtggatggtc acgacatcgt
     4141 gcgcgactac cgcgccgcgc gcgccctcgt cggcctggtg ccgcaggaac tcaccaccga
     4201 cgccttcgag acggtgtgga acacggtgag cttcacgcgc ggcctgttcg gcaagccgcc
     4261 cgacccggcc tacatcgagc agctgctgaa ggacttgtcg ctgtgggcga agaaggatgc
     4321 caggctgatg atgctctcgg gcggcatgaa gcgccgcgtg ctgatcgcca aggcgctctc
     4381 gcacgagccg cgcatcctct tcctcgacga gcccaccgcc ggggtggacg tggagctgcg
     4441 ccgcgacatg tggcagctcg tgcgccgctt gcgcgacagc ggcgtcaccg tgatcctgac
     4501 cacgcactac atcgaggagg cggaggagat ggccgaccgc atcggcgtga tcgccaaggg
     4561 tgcgctgatc ctggtcgagg acaaggccat gctgatgacg aagctcggcg gcaagcagct
     4621 cacgctgcaa ctggcggcgc cgctcgccga ggtcccgccc gcgctcgcgc gctacgggct
     4681 ggtgctgggc aagggcggca aaacgctggt ctatcgctac gaggtcggca ggggcgaagg
     4741 cggggaaggg ggcgagggca gcgatccggc aggcgatggc gcgatcgcag gcttgctgca
     4801 ggacctcgcc gccgcgggga tcatgttcac cgacctgcac actacccagc gttcgctcga
     4861 agagatcttc gtcagcctgg tgagcgagcg cgaagcgccg ggtacggacg agcggggcaa
     4921 ccgggccgag gccgcagggg agggcgcacg atgaactggc acgcggtccg cgcgatctac
     4981 ctgttcgaga tggcgcgcac gaagcgcacg ctgctgcaga gcgtgctggc gccggtgatc
     5041 accacctcgc tgtatttcgt cgtcttcggc tcggcgatcg gcgcgcgcat ccccgagatc
     5101 gagggcgtga gctacggcgc cttcatcgtg cccggcctga tcatgctcaa cctgctcacc
     5161 cagagcgtgt cgaacgcctc cttcggcatc ttcttcccga agttctcgcg cacgatctac
     5221 gagatcctgt cggcgccggt gtcgcacttc gagatcgtgc tgagctacgt gggcgcggcg
     5281 gcgaccaagg cggtgatcct gagcctgatc atcctcgcca ccgccacctt cttcgtgccg
     5341 ctgcgcatcg agcatccgct gtggatgatc gccttcctgc tgctcaccgc ggtgagcttc
     5401 agcctgctcg gcttcatcct cggcatctgg gcggacaact tcgagaagct gcagctcgtg
     5461 ccgctgctga tcatcacccc gctgaccttc ctcggtggca gcttctactc gatcgacatg
     5521 ctgccggcgg ggtggcgcac ggtgtcgctg ttcaaccccg tggtgtacct ggtgagcggc
     5581 ttccgctgga gcttctacgg cagcgcggat gtggcggtgg gggtgagcct ggcgatgacg
     5641 ctgctgttcc tggcggtgtt cgtgggggtg gcgacctgga tgttgaggac ggggtatcgg
     5701 ctgaagcctt gaggggcgca ggcccgccgg ctggcgtcgc atccggggtg ccgcatcccg
     5761 ggcgtcggaa tcgcgacttg cgtcgctccc acgggagatt ccggaccggt gcgttttccg
     5821 gtcaccgcgg cgattccgtc taccgccgcg ggtccgtgct tctcccgtgg gagcgacgca
     5881 agtcgcgatt gcagcggtga cggatcgcca ccactgcaat cgcttgcgac ggaggtgccg
     5941 cggcgcaagg cccggcgcct cgccccttac gggtacagcc cgcgctccgc gcgcgcctcc
     6001 agcacccgcg cgcaggcgat gatgaaggcg gcggtgcgca gcgacacctg cttctcctgg
     6061 gccaccttcc agatcgcggt gaaggcggcg accatgatgc gctcgaggcg ctcgttgatt
     6121 tcgtcctcgg tccagaagaa gctggagaaa tcctgcaccc attcgaagta cgacacggtg
     6181 acgccgccgg cgttggcgag cacgtcgggc acgaccagga tgccgcgctc gtggaggatg
     6241 tcgtccgcgg ccggggtggt cgggccgttg gcgccctcga cgacgatgcg cgcacgcgcg
     6301 ccggcagcac gctcgacggt gagctggcct tcgagcgcgg cagggacgag aaactcgcac
     6361 tcgaggcgcc agaacgcctc ggggtcgatc ggcgcggcgc cggcgaagcc cttcaggccg
     6421 ccgttcgcgg cggtgtgctc gagcgcggcg gggatgtcga tgccggcctc gttcaccagg
     6481 atggcggtat ggtcggcgat cgcgatcacg cgcgcgccgg cgtcgtggaa catgcgcgcg
     6541 ccgatgccgc cgacgttgcc gaagccctgc acgaccacgc gcgcgccctc gatcggcagg
     6601 cgcaggtggc gggcggcctc gcgcgcggcg atgaagacgc cgcggccggt ggcttcctgg
     6661 cggcccaggc tgccgccgag cgcgatcggc ttgccggtga cgacgccggt ggcggtgccg
     6721 ccgcggttca tcgagaaggt gtccatcatg atcgccatcg tcatcgcgtt ggtgccgacg
     6781 tcgggcgcgg ggatgtcctt gtccgggccg atgatgatgc cgatctcgga ggtgtagcgg
     6841 cgggtgatgc gctgcagctc gcccttgctg acgctcgccg gatcgacgcg gatgccgccc
     6901 ttggcgccgc cgaagggcag gccgaccgcg gcgttcttga tcgtcatcca gccggccagc
     6961 gccatcacct cgttgagggt gacgtcgggg tggaagcgca cgccgccctt gcccgggccg
     7021 cgcgacaggt tgtgctgcac gcggtagccc tcgtagtggg cgacggtgcc gtcgtcgcgc
     7081 tcgatcggca catcgacgat cagcgcgcgc ttgggatgct tgagggtctc gatccagcgt
     7141 tcgagcgagc cgagatgggg gcgcacgcgc tcgatctgct cgacgaagct cgcccacggc
     7201 tcgggcgccg ccgggttcag gtaggaaagg ccgccgacgc ggtcgagggc cgccttgtct
     7261 tgtggtgtcg cactcatctg tctgcctcgt gtggtgtgag aaggtgcgcg cattgtgtgg
     7321 attgccctgg cccgaaggca tgccgttgct acatcgggtc atgcgtcttg cgcatagggt
     7381 ggccggaggg gccggtgagt cggtgtgcag ccgcgtgcct cggctacccg cggcgctcgg
     7441 gttctggtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aggctttgcg
     7561 gcgaggtgct gccacaggcg cagcagctcg ggcttggtgt tggcgcggtc gcggtagagg
     7621 cggacctcca tctccaggct ccactccggc ccgccggcga ccaccacctc gccgcgttcc
     7681 acctcgcgcg cgaccgcgct cgcgggcagg aaggcgaggc cgtgaccggc gagcagcatg
     7741 gccttgaggc tctcggccat gtcggactcg tactgcaggg cgaggtgggc cggatgggcg
     7801 gcgcggtcga ggatggtgtc gaccatgcgg cgcaggtagg tgttgggtcc gtagcgcagg
     7861 aaggggatgg gcgcctcggc cgaaccgggc agctgccagc ccgcgctgcg cgcgagccgg
     7921 cgcggcaggt aggggcgcac gcactcgacc gcgagctgga tgccttcgaa gcgcgcggga
     7981 tcgagccaca ccgggtgggt cggatggtgg tagcacagca gcagatcgca gccgccttcc
     8041 accagcatgg tcaccacgtc atgcacgttg cccgcgatca gccgggtggg gacctcgccg
     8101 aactcggcct tgagccgccc cagccaggtc gggaagaagg cgaaggcgag cgtgtgcggg
     8161 acggcgaaca cgagcgcgtc gcccgcggtc gtctggtggc cgcgcaccag cgcgcgcgcc
     8221 gcatgcaggc ggtggatgag atccgccgcc ggtcccttga aggcctcgcc cgcttcggtg
     8281 agccgggtcg ggaaggtgct gcggtcgacc agcgccaccc ccagccaggc ctcgagcgcg
     8341 cggatccggc gcgagaaggc gggctgcgag acgtggcgct gctcggccga gcgcgagaag
     8401 ctcccgctgt cggcgaggct gaggaaatct tccagccatt tgagttccat gggcgggccc
     8461 tgcaggggga gagaccccat tgtatgcagg aaatccgccg gcgttccccg cgtaccgccg
     8521 ctcggcgccc gtcgcccatc gggcccggtg ctcgccgggc cggggcagga cgcgcgcggg
     8581 cggcatgccc gggtatgcat tcgggattcg caggccccat aatggggcgt ttcgtgaact
     8641 ggttcgcatg attcaagtaa ccgggcgcga ctgcgccgca aaattgcggc agagttcgcg
     8701 cactgccccc caatccccca tgcgccggag agcccccgtc gacgcccgtc gcgggacgac
     8761 cgatgatccg tacctcgctc aatttccgcc tgtggctgac gatcgcgatc gcggtacttc
     8821 cggtgctgct gttcgtcttt ttcgacttcc acgagcgccg cgaacaggcg gtggccgaga
     8881 cccgtgccga gatcgcactg cgcctggcgg atgcgaaccg cgaggcgcag gccgcgcatc
     8941 gcgcggtcgc gttggtgttg cggatcatgg cgcgctccaa cgacctgcag gcgctggatt
     9001 ccgcgcaatg ctcgggcatc gccgggcgcc tgctcgagtc gctggacggt ttcgcaaaca
     9061 tcggcgccgc gctgcccgac ggggagatct tctgcagcgg gaggccgatg agcgccccgg
     9121 tgacggtgat cgatcgcgcc tggttccgca cttctctcga cagtgatgaa atttcccccg
     9181 gggagtttgt catcggcagg gtgtcgggaa cgccgggcat ggctttcggc tacccgctgc
     9241 gtgcggcgga cggcacgctg cgtgcggtga tcttcgccac catcacggtg gcgtggttcg
     9301 atcgcctgat cgaagggttc cgcctgcctg agggttggga ggcgagcgtg atcagcaatt
     9361 ccggcctggt gctcgcgcac cagcccgagc gtgcgcccga gggctggcgc gcgcacaagg
     9421 tccccgcgac gacgctcgcg cgcatggccg ccgtcgtgcg cgacgaaggc ggcatcgccg
     9481 aactcgccgg catcgatggc gtgcagcgcc tgtacggcat cggcgtgccg gatttcgccc
     9541 ctggcagcgg ctttttcgcg atcggtgcgc cgctggagcg ctcgctggag gcggcggggc
     9601 agcgcctgct gatgtacctg gccctggtcg cggccatcgc gctggcctcc gcgctgatcg
     9661 cgcgcttgta catctaccgg ctgatcgagg tgtgggcgac gcgggtgcgc ggtgcgattg
     9721 ccgagatcgc cgccgggcgg cgcgatgcgc gcatcgggca ggccaccggc gttgccgagc
     9781 tcgacgcgct ctccgagggc atcgaccaga tggcgttgga gatcggcaag cgcgagggcg
     9841 agctgcaacg cctgtcgatg gtgatcgagc agagcccgga gtcgatcgtc atcaccgaca
     9901 ccgcggggtg catcctgtat gtgaacgagg ccttccagcg catcacgggc tatgcgcgcg
     9961 gcgaggtgct cggacgcaac ccgcgcatcc tcaacggcgg gttgacgccg cgcgcgacct
    10021 acgagcagat gtgggcgacc ttgcttgcag gcgaggtgtg gcgcggcgag ttccacaacg
    10081 tccgcaagga cggctccgcc tatctcgagc tggcgacgat cgcgccgatc aaggatgcgg
    10141 gcggcacggt cacccactac gtggcggtca aggaggacat cacgcagcgc aagcaatccg
    10201 aggcgctgct gcaccggctg gcctacttcg atgcgctgac cgggctgccc aaccgcgcgc
    10261 tgctgcacga ccgcatcgcc caggccatcc gcagcggtgt gcgccgcgag agcttcggca
    10321 tgctgatgct ggtcgacatc gatcgcttcc gccagctcaa cgacaccctc ggtcacgccg
    10381 ccggagaccg cctgctgtgc gaggtcgcgg cgcgcctgcg cgcgagcgtg cgcgaggagg
    10441 acacggtcgc gcgccatggc gacgacgact tcgccgtgct cgtcgagcgc atcggcgaaa
    10501 ccgaggccga tgcgctgagc cacgccgagt acgtcgcacg caagctccag cgcacgctgc
    10561 aacaacccta tgtgctcggt ggtacggagg gcgaccgcca cttcgcgacg ctgagcttcg
    10621 gcatcagcct gttccacgac ggcgtgagct cgctcgagag cctgctcaag caggccgagg
    10681 tggccttgta ccgggccaag caggacgggc gcgacatcgt gcgcttcttc aaccccgaga
    10741 tgcaggcggt ggtcgatgcc cacgcccgtc tcgaggcccg catgcacgag gcgctggagg
    10801 cgaacgcctt ccggctcttc ttccagcccc aggtcaaccg gcgcggcgtg gtgatcggcg
    10861 ccgaggccct gctgcgctgg ccgctcgacg gtggcgccat ggtgtcgccg gccgagttca
    10921 tcccgctcgc cgaggacacc ggccacatcg tccgcctcgg ctcgtgggtg ctgcgcagcg
    10981 cctgcgccca gctcgcgcgc tggcagatga acgagaccac ccgccacctc aagatcgcgg
    11041 tcaacgtcag cgcgcgccag ttccaccagc ccgacttcgt cgccagcgtg aaggacaccg
    11101 tgcgcgccgc gggtgtcgac ccctgccgcc tggagctgga actcaccgag agcgccatcc
    11161 tgtcggacat ggacgagacc atccggcgca tgaacgagct gcgcgcgctc ggcatccgct
    11221 tcgcgctcga cgacttcggc accggctact cctcgctgtc ctacctcaag cgcctgccct
    11281 tggaccagtt gaagatcgac cagtccttcg tgcgcgacat ggccgaggac gagggcagcg
    11341 aggcgatcgt gctggcgatc ctgtcgctga gccacgcgct cggcctgga
//
LOCUS       DAOQNN010000053        11386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2607_length_11386_cov_9.138028, whole genome shotgun sequence.
ACCESSION   DAOQNN010000053 DAOQNN010000000
VERSION     DAOQNN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11386
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2607_length_11386_cov_9.138028"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..293)
                     /locus_tag="PKV97_05025"
     CDS             complement(63..293)
                     /locus_tag="PKV97_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNB05274.1"
                     /translation="MRTVAVVEAKSQFSALLAAVERGEEVAITRRGRVIARLIPDTPR
                     SAADLFRVCWQEGGLDLEAPPDAPAEPVAGWD"
     gene            complement(376..792)
                     /locus_tag="PKV97_05030"
     CDS             complement(376..792)
                     /locus_tag="PKV97_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNB05275.1"
                     /translation="MPARYLLDTNILSSLIKDPQGPVTKRLAQLDADAICTSIVVACE
                     LRYGAAKRGSVVLTQRVEDLLGAIEVMPFEGDADRQYAEIRATLERAGNTIGANDLLI
                     AAHARTLDLTLVTHNLREFERVPGLRLEDWLERKDE"
     gene            complement(792..1031)
                     /locus_tag="PKV97_05035"
     CDS             complement(792..1031)
                     /locus_tag="PKV97_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNB05276.1"
                     /translation="MNSERHVSLFRNGRNQAVRIPREFELEGTEAIIRKEGARLIIEP
                     IGPRHRLLALLAGWGPLQEAFPEIADPPPEPEDVF"
     gene            1368..1616
                     /locus_tag="PKV97_05040"
     CDS             1368..1616
                     /locus_tag="PKV97_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNB05277.1"
                     /translation="MSDDRQAHIAQKAPYAVEVEAGKKYFWCACGLSKNQPFCDGSHK
                     GGPFSPLAFTAEKDGTVHLCGCKHTAKAPYCDGTHTKL"
     gene            1792..2355
                     /locus_tag="PKV97_05045"
     CDS             1792..2355
                     /locus_tag="PKV97_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNB05278.1"
                     /translation="MRPLLTCLALLLPVLAFGADADEAALWKALRDGGHVALMRHAIA
                     PGVGDPAGFRLDDCTTQRKLSARGRAQARAIGERFRANGIATAAVFSSQWCRCIDTAR
                     ELALGEVVAFPGLNSFFADRGEEARHTAAVRALIGERARSPLPLVLVTHQVNITALTG
                     VFPASGEIIVLRVDGDTPSVAGRIRTD"
     gene            complement(2416..2988)
                     /locus_tag="PKV97_05050"
     CDS             complement(2416..2988)
                     /locus_tag="PKV97_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNB05279.1"
                     /translation="MSLPQPKIPFGPDDYLAWEQDQPTRSEYVDGEVFAMSGASDAHG
                     TAAGNLFAGLHAHLRGTPCKPFIADMKVHVEAANSFFYPDILVTCDPRDRSAEASHVK
                     RHPLLIVELLSPSTEAYDRGNKFAAYRTLAALQEYVLVSLEQRRIEVFRRDDSGHWVL
                     YPFALDEQLELASVGLRCPVAEVFEGVEGE"
     gene            complement(3102..3659)
                     /locus_tag="PKV97_05055"
     CDS             complement(3102..3659)
                     /locus_tag="PKV97_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNB05280.1"
                     /translation="MFLLDWLFGKHQAKAEAAPSSATVPAPEPAHSMEDSESFAPGTT
                     IRYSPELVERLVRDHRLMLERFGAIREAAHAGRFDEVVPLLERFRVDLHAHLLVENVR
                     LYVYLEHTLADDPVSHALMHEFRHDMDEIGKKVIAFLARYVRLGEDPALAASFAADLE
                     RVGAVLVDRIQREEDTLYPLYAPVS"
     gene            3874..4221
                     /gene="cutA"
                     /locus_tag="PKV97_05060"
     CDS             3874..4221
                     /gene="cutA"
                     /locus_tag="PKV97_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNB05281.1"
                     /translation="MPASSATTPRALLVFTNLPDAASAEALANALIDRQLAACVNMLA
                     PCTSVYRWQGATETATEVPLLIKTTPARYAALEAAIRAHHPYELPEIIAVPVTQGLPG
                     YLDWVAAGTRDDD"
     gene            4315..6159
                     /gene="dsbD"
                     /locus_tag="PKV97_05065"
     CDS             4315..6159
                     /gene="dsbD"
                     /locus_tag="PKV97_05065"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNB05282.1"
                     /translation="MHRLFLLLALIASLLLPAAARANPIEPEKAFAMRAQALDAQTIE
                     VVFEIAKDYYLYGDKFRFEAEPAGVGFGAMEKPAGKKHKDDFFGEVETHRGELRILVP
                     VQAPAGTTRFELFATSQGCWDGGICYPPTTQQASIDLAAPPKKAGGFLDSVLGGRAGT
                     GSAASPTPVALAAPEDGSGGGGAVSSDESGDIARLLSGASVPIILLSFFGFGLLLAFT
                     PCTFPMIPILSGIIVGQGNKVSHTRAFVLSLAYVLGMAVTYALAGVAAGMTGTMLSAA
                     LQNVWVLSTFALMFVLLSLSMFGFYELQLPSTLQSKLADTASHGKGGHLGGVTLMGVL
                     SALIVGPCVAAPLAGALLYIAQTGDAVLGGWALFAMGLGMGAPLLAVGVASRSLLPKV
                     GPWMEGVKKAFGVMLLAVALWMITPVIPPLATMLGWAALLLFSAIFLHAIDPLPPQAK
                     SWQRFWKGAGVVLLLAGAAILVGALAGSRDPLQPLSVLRAQAAAPVDVPQFEKVDSIA
                     ELEARLATTDRPVLLDFYADWCVSCKEMERFTFSDAAVAARMGHMLLLKADVTANTDE
                     HKALLKRFGLFGPPGILFFDGQGKERAGLRVVGFMKAEPFATVLDRAL"
     gene            6218..6826
                     /locus_tag="PKV97_05070"
     CDS             6218..6826
                     /locus_tag="PKV97_05070"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNB05283.1"
                     /translation="MFSGIVAAVGRIEHVAALADGVRITVDTNGLDLSDVIVGDSVAH
                     SGVCLTVVEMDGPRLRYDVSRETLNCTVGLDAVGGEVNLEKSLRLADRLGGHLVTGHV
                     DGVGEVVKFAPIGESHELVIRAPAAIAGYIAKKGSVTVNGVSLTVNRVEGRDFWINLI
                     PHTVAVTNLKHLAPGSKVNLEIDLIARYVERMLEWKNDAAQA"
     gene            6861..7952
                     /gene="ribBA"
                     /locus_tag="PKV97_05075"
     CDS             6861..7952
                     /gene="ribBA"
                     /locus_tag="PKV97_05075"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNB05284.1"
                     /translation="MSALAPITDIIADIKAGKMVILVDEEDRENEGDLVMAAEFVTPE
                     AINFMAKHGRGLICLTLTDERCRQLGLQQMVRDNRTPHGTAFTVSIEAATGVTTGISA
                     HDRARTVQVAVARHARPEDIVTPGHIFPLTAQKGGVLIRAGHTEAGCDLAQLAGLEPA
                     AVICEILKDDGTMARLPDLVEFGQAHGLRIGAIRDLIEYRAANDNLVDKVAEKDVDTA
                     HGRFRLTAFEDKTSGDVHFALSHGDIRPERETLVRVHEPISVLDFLDAGSGHHTFPVN
                     QALATLAAAEAGVIVLLYRPMSGRELLAGLDGGHDRPTAKWDARLFGVGAQILRALRV
                     GKMRLLASPRKIPSMTGFGLEITGFVDKN"
     gene            7970..8473
                     /gene="ribH"
                     /locus_tag="PKV97_05080"
     CDS             7970..8473
                     /gene="ribH"
                     /locus_tag="PKV97_05080"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNB05285.1"
                     /translation="MQRNQSENARQGARYDGIDEIEPNFAGAGVRVGIVMARFNLDIC
                     EGLLSACTDELLALGVAREDIRIVTVPGALEIPLVLQTMARSGRHDALVALGAVVRGE
                     TYHFELVSNEMGAGVTRVGLDTGLPIANGVLTTEDDDQAVARMHEKGADCARAAVEMA
                     NLLKVVR"
     gene            8470..8970
                     /gene="nusB"
                     /locus_tag="PKV97_05085"
     CDS             8470..8970
                     /gene="nusB"
                     /locus_tag="PKV97_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNB05286.1"
                     /translation="MSDIDNTQAEGAAGAPNPTSKMARRRARELALQGVYQWLLSGNN
                     ATTVQRHLESETENLDKIDRELFVSLLRGATGAADELRGCFEPLLSRPVAELSPIEHA
                     ILLLGTHELRHNLDTPYRVVINEAIELAKGYGGTDGHKFVNGVLDKLSTRLRPEEVEA
                     ARAKRG"
     gene            complement(9269..>9450)
                     /locus_tag="PKV97_05090"
     CDS             complement(9269..>9450)
                     /locus_tag="PKV97_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNB05287.1"
                     /translation="RLSPLGATKMACEPRAMEDEQRFMAAAARVTGFALAGDGRLELR
                     AGDRVVMRARRMGGR"
     assembly_gap    9451..9550
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9551..10443)
                     /locus_tag="PKV97_05095"
     CDS             complement(<9551..10443)
                     /locus_tag="PKV97_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNB05288.1"
                     /translation="MKTSSLLGLGLLAGLVGGCSLLSPAAPALAAEASPQAVAERPAD
                     PTPALDPQPPLEFRCGERRVELRHFGELATLGVDGKVFTLRPERSASGMRMVAVDDER
                     TGIWVKGNSARLSLAGVEQPECRRTAAKPLFRAVGNEPAWRLDIGAQGLELIADGGAS
                     RAFASAPLIVEVEGMRSYQGTSAGGELEALVFDRVCVDSMSGMTHPNSVEVRWQDRVL
                     RGCGGEPAQLLQGEPWAVVELDGRAVADPARVTLGFAADDRLAGLAACNRYFGSYVLS
                     GEGLRLSPLGATKMACEPRAM"
     gene            10651..10992
                     /gene="hypA"
                     /locus_tag="PKV97_05100"
     CDS             10651..10992
                     /gene="hypA"
                     /locus_tag="PKV97_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNB05289.1"
                     /translation="MHEMSLAEGVRTLFEDAAAADGFGHVRAVVLEIGELAAVEVEAL
                     RFCLDVVLADTIAAGAAIEVETLPGAGWCPACAQEVPIHQLYDPCPHCGGYRVQTTAG
                     REMRVKALEVD"
BASE COUNT         1775 a         4102 c         3764 g         1645 t
ORIGIN      
        1 gcaggatgcg ccttgagcgc cgcgatgaag atgttggtat cgagcagata gtgcatgccg
       61 cgtcaatccc agccggcgac cggctcggca ggcgcatcgg gcggcgcctc caggtccagc
      121 ccgccctcct gccagcagac ccggaacagg tctgccgcgg aacgcggcgt atccggtatg
      181 aggcgggcga tcacccgacc gcgccgggtg atcgccacct cctcaccacg ctcgaccgcc
      241 gccagcagcg ctgaaaactg gctcttggcc tcgacaactg caaccgtgcg catgacggct
      301 ctccagatga ccacatggac agatgatagc agcggttgtg ggcgagaaag aaacccccag
      361 ggtcaggtct ttcatctact catctttccg ctccagccaa tcctccagcc gcaagcccgg
      421 aacgcgctcg aattcacgga ggttatgcgt gacgagggtg aggtcgagcg tgcgagcatg
      481 cgccgcgatc agcaggtcgt tggctccgat ggtgttcccg gcccgctcca gcgtcgcgcg
      541 aatttcggcg tactggcgat cggcatcccc ctcgaagggc atcacctcga tggcaccgag
      601 caagtcttcc actcgctgcg tcaggacgac cgagccgcgc ttcgccgcgc cataacggag
      661 ctcacaagcc acgacgatgc tggtgcagat ggcgtcggcg tcgagttggg ccagccgctt
      721 cgttaccggc ccctgcggat ccttgatcag gctcgacagg atgttcgtgt ccagcagata
      781 acgcgccggc atcagaacac gtcctcgggt tcaggcggcg ggtcggcgat ttcggggaag
      841 gcttcctgca gcgggcccca gccggccagc aaggccagga gcctgtgacg cgggccaatg
      901 ggctcgatga tcaaccgcgc cccttccttg cggatgatgg cctcggtgcc ttcgagctcg
      961 aactcgcgcg gtatccggac cgcctggttg cgtccgttgc gaaaaaggga gacgtggcgt
     1021 tcgctgttca tggtgacccc ctaaaagacc tatgccaaag cctatgcctt tcagaagctc
     1081 gggtcaatca gcagaaacgc gagctcagga gcgaaattgc aacacaggga acagacccca
     1141 atttcctccc ggtgccatta ctacacgccc ccatgacgac gccagaactc ggcgggcccg
     1201 agacagcagg cgccctgatc gccaaatgct ggaagccgtc gccagttcat cgaaacgcgc
     1261 gaccgccgcg aaccgacgga cgattgactc gccccacgca gaggcgcacc gtttgtcgac
     1321 ctccggctgc cgtcggaggc gaacagcccg acagaggaga cgagaccatg tccgacgatc
     1381 gccaagccca tatcgcgcag aaggctcctt atgccgtcga ggtcgaggcc ggcaagaagt
     1441 acttctggtg tgcctgtggc ctgagcaaga accagccctt ctgcgacggc tcgcacaagg
     1501 gcggcccctt ctcgccgctg gccttcaccg cggagaagga cggcacggtg catctgtgcg
     1561 gctgcaagca caccgccaag gcgccgtatt gcgacgggac gcacacgaaa ttgtgagctc
     1621 gccggacccc gtgtaggagc ggcgccagca gcgaacaccg cgcttccgat gcgcgccacg
     1681 cccgtttcac caccgcattc gcgactggca gcgctcctac ataaagccgc ggcacttttg
     1741 cgccgcgacg cggtctctgc agccggttac gcctgccccg gacactcccc gatgagaccc
     1801 ctcctcacct gcctcgccct gctgctgccc gtcctcgcct tcggtgcgga tgccgacgag
     1861 gccgcgctgt ggaaggcctt gcgcgacggc ggacatgtcg cgctgatgcg ccatgcgatc
     1921 gcacccggcg tcggcgaccc ggcaggtttc cggctggacg actgcaccac ccagcgcaag
     1981 ctgtcggcgc ggggtcgggc gcaggcgcgc gcgatcggcg agcgcttccg cgccaacggc
     2041 atcgccaccg cggcggtgtt ctccagccag tggtgccgct gcatcgatac cgcgcgcgaa
     2101 ctggcgctgg gcgaggtggt ggcgttcccg gggctgaact cgttcttcgc cgaccgcggc
     2161 gaggaggcgc ggcacaccgc tgcggtccgc gcgctgatcg gggaacgcgc gcgcagtccc
     2221 ttgccgctgg tgctggtgac gcatcaggtg aacatcaccg cgctgacggg ggtgtttccg
     2281 gcctcgggcg agatcatcgt gctgcgggtc gacggcgaca cgccgagcgt ggccggccgc
     2341 atccggaccg attgagacag ccggctcgcc tcttcttgcc gtgcatcgcg gggacggcga
     2401 aaacgcccac ccgcgctact cgccctccac cccttcgaag acctcggcca ccgggcagcg
     2461 caggccgacg ctcgcgagtt ccagctgctc gtcgagggcg aaggggtaga gcacccagtg
     2521 cccactgtcg tcgcgccgga agacctcgat acgacgctgc tcgagcgaga cgaggacgta
     2581 ctcctgcagc gcagccagcg tgcggtaggc ggcgaacttg ttgccgcggt cgtaggcctc
     2641 ggtggacggc gagagcagct cgacgatcag cagcgggtga cgcttgacgt ggctggcctc
     2701 ggcgctgcgg tcgcgcgggt cgcaggtcac gaggatgtcg gggtagaaga agctgttggc
     2761 cgcctcgacg tggaccttca tgtcggcgat gaagggcttg cagggggtac cgcgcagatg
     2821 cgcatgcagg ccggcgaaaa ggttgccggc tgccgtaccg tgcgcgtccg acgcgccgct
     2881 catggcgaag acctcgccat cgacgtattc gctgcgggtg ggctgatcct gttcccaggc
     2941 caggtagtcg tcggggccga acgggatctt gggctggggc aggctcatcg ggcgtcctcg
     3001 ctgcaattcg ttgcggtttg cgcttcgagc atagcggcga gggacgggac aggcaatcga
     3061 gaggcccgtc gtccgggtgg tcggacgacg agtgcagcgg ctcagctcac cggtgcatag
     3121 agcggataca aggtgtcctc ctcgcgctgg atgcggtcga ccagcacggc gccgacgcgc
     3181 tcgaggtcgg cggcgaagct cgcggcgagc gccgggtctt cgccgaggcg gacgtagcgg
     3241 gcgaggaagg cgatcacctt cttgccgatc tcgtccatgt cgtggcggaa ctcgtgcatc
     3301 agcgcgtggc tcaccggatc gtcggcaagg gtgtgctcga gatacacgta caggcgcacg
     3361 ttctcgacca gcagatgggc gtgaaggtcg acgcggaagc gctcgagcag gggcacgacc
     3421 tcgtcgaagc gccctgcgtg cgcggcctcg cggatggcgc cgaagcgttc gagcatcaga
     3481 cgatgatcgc gcaccaggcg ctcgaccagt tcggggctgt agcggatggt ggtgccggga
     3541 gcgaagctct cggagtcctc catggaatgc gcgggttcgg gggcaggaac cgtggcggac
     3601 gagggcgcgg cctccgcctt ggcctggtgc ttgccgaaca gccagtcgag caggaacatg
     3661 atgtgctccg gatggagccg cggagggcgg ccgatgcgtg aaggattccg acgctaatac
     3721 aggtgcatga tggacttcca tggcacagat caaatccgcg ccggatccat ccgcgacacc
     3781 ggcgtcaccc ggaattttgt gcttgatccc gcgcagggcg tccgcgcccg cgctgcggga
     3841 taatcgcggg cgttccacga cggatccgcc accatgcccg ccagctcagc caccacgccg
     3901 cgcgccctgc tcgtcttcac gaacctgccc gacgccgcca gcgccgaggc cctggcgaac
     3961 gcgctgatcg accggcagct cgccgcctgc gtgaacatgc tcgcgccctg cacctcggtg
     4021 tatcgctggc agggcgcgac cgagaccgcg accgaggtac cgctgctgat caagaccacg
     4081 ccggcgcgct acgccgcgct ggaggcggcg atccgggccc accaccccta cgaacttccc
     4141 gagatcatcg cggtccctgt gacgcagggc ttgcccggct atctcgactg ggttgccgcc
     4201 ggaacgaggg acgacgactg aacgcccgcc ggcgcgtccc cggcatcgcc ccgcccgatc
     4261 gacccgtccg caggcgccgc agcgcgcccg cccaccgccg cctagaagac cacgatgcac
     4321 cgccttttcc tcctgctcgc cctgatcgcg agcctgctcc tgcccgccgc ggcccgcgcc
     4381 aacccgatcg agcccgagaa ggccttcgcg atgcgcgcgc aggcgctcga cgcgcagacc
     4441 atcgaggtcg tgttcgagat cgccaaggac tactacctct acggcgacaa gttccgcttc
     4501 gaggccgagc ccgccggcgt gggcttcggc gcgatggaaa agcccgcggg caagaagcac
     4561 aaggacgact tcttcggcga ggtcgagacc caccgcggcg agctgcgcat cctggtcccg
     4621 gtacaggcgc ccgccggcac cacccgcttc gagctcttcg ccaccagcca gggctgctgg
     4681 gatggcggca tctgctatcc gcccaccacg cagcaggcct cgatcgacct cgccgcgccg
     4741 ccgaagaagg cgggcggctt cctcgacagc gtgctcggcg gccgcgcggg caccggcagc
     4801 gccgcttcgc ccaccccggt cgcgcttgcc gcgccggagg acggcagcgg cggcggcggc
     4861 gccgtctcca gcgacgagag cggcgacatc gcccgcctgc tttccggcgc cagcgtaccg
     4921 atcatcctgc tgagcttctt cggcttcggc ctgctgctcg ccttcacgcc gtgcaccttc
     4981 ccgatgatcc cgatcctgtc gggcatcatc gtcggccagg gcaacaaggt ctcgcacacg
     5041 cgcgccttcg tgctctcgct cgcctacgtg ctgggcatgg cggtgaccta cgcgctcgcc
     5101 ggggtcgccg ccggcatgac cggcaccatg ctgtcggcag cgctgcagaa cgtgtgggtg
     5161 ctgtcgacct tcgcgctgat gttcgtgctg ctgtcgctgt cgatgttcgg cttctacgag
     5221 ctgcagctgc ccagcacgct gcagagcaag ctggccgaca ccgccagcca tggcaagggc
     5281 ggccacctcg gcggcgtcac cctgatgggc gtgctgtcgg ccctcatcgt cggcccctgc
     5341 gtggcggccc cgctcgccgg cgccctgctc tacatcgcgc agaccggcga cgccgtgctc
     5401 ggtggctggg cgctgttcgc gatggggctg ggcatgggcg cgccgctgct ggcggtgggc
     5461 gtggcctcgc gcagcctgct gcccaaggtc gggccgtgga tggaaggcgt caagaaggcc
     5521 ttcggcgtga tgctgctcgc ggtggcgctg tggatgatca ccccggtgat cccgcccctg
     5581 gccaccatgc tgggctgggc cgcgctgctg ctgttctcgg cgattttcct gcacgcgatc
     5641 gacccgctgc cgccgcaggc caagagctgg cagcgcttct ggaagggtgc gggcgtggtg
     5701 ctgttgttgg ccggcgcggc gatcctcgtg ggcgcgctcg ccggctcgcg cgacccgctg
     5761 cagcccttgt cggtgctgcg cgcgcaggcc gcggcgccgg tcgatgtgcc gcagttcgag
     5821 aaggtggact ccatcgccga actcgaggcc cggctcgcca ccaccgatcg cccggtgctg
     5881 ctcgacttct atgccgactg gtgcgtgtcg tgcaaggaga tggagcgctt caccttcagc
     5941 gacgccgcgg tcgccgcgcg catgggccac atgctgctgc tgaaggccga cgtcaccgcc
     6001 aacaccgacg agcacaaggc gctgctcaag cgcttcggcc tcttcggccc gcccggcatc
     6061 ctcttcttcg acggccaggg caaggagcgc gcgggcctgc gcgtggtggg cttcatgaag
     6121 gcggagccgt tcgcgaccgt gctggaccgg gcgctctgag cgaggccggg gtttttcccc
     6181 tacaatcccg cctttcccct tacggttcaa acccttcatg ttctccggca tcgtcgcggc
     6241 cgtcggccgc atcgaacacg tcgcggcgct cgcggacggc gtgcgcatca ccgtcgacac
     6301 caacggcctc gatctttccg acgtgatcgt cggcgacagc gtggcgcaca gcggcgtgtg
     6361 cctcaccgtg gtcgagatgg acggcccgcg gctgcgctac gacgtctcgc gcgagacgct
     6421 caactgcacc gtggggctgg acgccgtcgg cggcgaggtc aacctcgaga agtcgctgcg
     6481 cctggccgac cgcctcggcg gccatctcgt caccggccat gtggacggcg tcggcgaggt
     6541 ggtgaagttc gcgccgatcg gcgagagcca cgagctggtg atccgcgccc cggcggcgat
     6601 cgccggctac atcgcgaaga agggctcggt caccgtcaat ggcgtcagcc tcacggtcaa
     6661 ccgcgtcgag ggccgcgact tctggatcaa cctgatcccg cacacggtcg cggtcaccaa
     6721 cctcaagcac ctcgcgccgg gcagcaaggt caatctcgag atcgacctca tcgcacgcta
     6781 cgtcgagcgc atgctcgagt ggaagaacga cgccgcgcag gcctgacccg cgcgccccaa
     6841 cgcaacacaa aggaagcagc atgagcgcac tcgcgccgat taccgacatc atcgccgaca
     6901 tcaaggccgg gaaaatggtc atcctggtcg acgaggaaga ccgcgagaac gagggcgacc
     6961 tcgtcatggc ggccgaattc gtcacccccg aggcgatcaa cttcatggcc aagcacggcc
     7021 gcgggctgat ctgcctgacg ctcaccgacg agcgctgccg ccagctcggc ctgcagcaga
     7081 tggtgcgcga caaccgcacc ccgcacggca ccgcgttcac ggtgtcgatc gaggccgcca
     7141 ccggcgtgac caccggcatc tcggcgcacg accgcgcgcg caccgtgcag gtcgcggtcg
     7201 cccgccacgc caggccggaa gacatcgtca ccccgggcca catcttcccg ctcaccgcgc
     7261 agaagggcgg cgtgctgatc cgcgccggcc acaccgaggc cggctgcgac ctcgcccagc
     7321 tcgccgggct cgagcccgcc gcggtgatct gcgagatcct caaggacgac ggcaccatgg
     7381 cgcgcctgcc cgacctcgtc gagttcggcc aggcgcacgg gctcaggatc ggcgccatcc
     7441 gcgacctcat cgagtaccgc gccgccaacg acaacctggt cgacaaggtc gccgagaagg
     7501 atgtggacac cgcccacggc cgcttccgcc tcaccgcctt cgaggacaag acctcgggcg
     7561 acgtgcattt cgccctgtcg cacggcgaca tccgccccga gcgcgagacc ctcgtgcggg
     7621 tgcacgagcc gatctcggtg ctcgacttcc tcgacgccgg cagcggccac cataccttcc
     7681 cggtcaatca ggcgctcgcg accctggccg cggccgaggc cggcgtgatc gtgctgctct
     7741 accgcccgat gtccggccgc gagctgctcg ccggcctcga cggcggccac gaccgcccca
     7801 ccgcgaagtg ggacgcgcgc ctgttcggcg tcggcgcgca gatcctgcgc gcgctgcgcg
     7861 tcggcaagat gcgcctgctc gccagcccgc gcaagatccc gagcatgacc ggcttcggtc
     7921 tcgagatcac cggcttcgtc gacaagaact gaccacgaga tcccccagca tgcaacgcaa
     7981 ccaatccgag aacgcccgcc agggcgcccg ttacgacggc atcgacgaga tcgagcccaa
     8041 cttcgccggc gccggcgtgc gcgtcggcat cgtgatggcg cgcttcaacc tcgacatctg
     8101 cgagggcctg ctgtcggcct gcaccgacga gctgctcgcc ctcggcgtcg cgcgcgagga
     8161 catccgcatc gtcaccgtgc ccggcgccct ggagatcccg ctcgtgctgc agaccatggc
     8221 caggagcggc cgccacgacg ccctggtcgc gctcggcgcg gtggtccgcg gcgagaccta
     8281 ccacttcgag ctcgtctcca acgagatggg cgcgggcgtc acccgcgtcg gcctcgacac
     8341 cgggctgccg atcgccaacg gcgtgctcac caccgaggac gacgaccagg cggtcgcgcg
     8401 catgcacgag aagggcgccg actgcgcccg cgccgcggtc gagatggcca acctgctgaa
     8461 ggtggtgcga tgagcgacat cgacaacacg caagccgaag gcgccgccgg cgcgcccaac
     8521 cccaccagca agatggcccg ccgccgcgcg cgcgagttgg cactgcaggg ggtgtaccag
     8581 tggctgctgt cgggcaacaa cgccaccacc gtgcagcgcc acctcgagtc cgagaccgag
     8641 aacctcgaca agatcgaccg cgagctgttc gtgagcctgc tgcgcggcgc caccggcgcg
     8701 gccgacgagc tgcgcggctg cttcgagccg ctgctgtcgc gcccggtcgc cgagctgtcg
     8761 ccgatcgagc acgcgatcct gctgctcggc acccacgagc tgcgccacaa cctcgatacg
     8821 ccctaccgcg tggtgatcaa cgaggccatc gagctcgcca agggctacgg cggcaccgac
     8881 ggccacaagt tcgtgaacgg cgtgctcgac aagctctcca cccgtctgcg cccggaagag
     8941 gtcgaggcgg cgcgcgccaa gcgcggctga acgcgttcgc attcgcgcgc gagcggggcc
     9001 gcacggcacg tgcatagccc gcgcacggcc ccggcgccaa agatcgcgac ttgcgtcgct
     9061 cctacggaaa accggcacag cacctgaagc ccccgtagga gcgacgcaag tcgcgatcac
     9121 ggcgccgcat cgaccggagc cgcggatcgc ggcgcgagcc cgccccaccc ccgacgcccc
     9181 cgtgggagcg acgcaagtcg cgatctctgc gctgcagcaa gcgccgcacc ggtgaacatg
     9241 tgatcgaacg cggcttcgtc tcccgccctc aacgcccacc catgcgccgc gcgcgcatca
     9301 ccacccgatc gcccgcacgc agctccagcc ggccatcgcc cgcgagcgca aatcccgtca
     9361 ccctggcggc cgccgccatg aagcgctgct cgtcttccat cgcacgcggc tcgcaggcca
     9421 tcttcgtcgc ccccagcggc gacagccgca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn tccatcgcac gcggctcgca ggccatcttc gtcgccccca gcggcgacag
     9601 ccgcaagccc tcgcccgaca gcacatagct gccgaaatat cggttgcacg ccgccaggcc
     9661 ggcgagcctg tcgtcggccg cgaagccgag cgtcacgcgc gccggatcgg ccaccgcgcg
     9721 gccgtcgagc tccaccaccg cccagggctc gccctgcagc agttgcgcgg gctcgccacc
     9781 gcaaccgcgc agcacgcgat cctgccagcg cacctcgacg ctgttcgggt gcgtcatccc
     9841 gctcatcgag tctacgcaga cgcggtcgaa caccagcgcc tccagctcgc cgcccgcgct
     9901 cgtgccctga tagctgcgca tgccctcgac ctccacgatc agcggcgcgg aggcgaacgc
     9961 acgcgaggcg ccgccgtcgg cgatcagctc cagcccctgc gcgccgatgt cgagccgcca
    10021 cgccggctcg ttccccaccg cgcggaacag cggcttcgcc gccgtccgcc ggcactccgg
    10081 ctgttcgacc ccggcgaggc tcagccgcgc ggagttgccc ttgacccaga tgccggtacg
    10141 ctcgtcatcc accgccacca tccgcatgcc cgaggcgctg cgctcgggcc gcagcgtgaa
    10201 caccttcccg tcgacgccga gggtggcgag ctcgccgaag tggcgcagct cgaccctgcg
    10261 ctcgccgcag cggaactcga gcggcggctg gggatcgagc gccggcgtgg ggtcggccgg
    10321 gcgctcggcc acggcctggg gggatgcttc ggcggccagc gcaggcgcgg cagggctgag
    10381 caggctgcag ccgccgacca ggccggcgag gagaccgagg cccaacaggg aagaggtttt
    10441 catcgcgaga ctcgaggaca gggaaagacg gtccgtcatt ctaggggtcc gccgccccct
    10501 gtgccttccc tcgccttccc tcgcccatcc gcccaagcac tcgccgcccc gcgccgcccg
    10561 ccggctgccc ccggcgcttt gcgcagtccg ccttcgcgtt tcgcttctct ccgccgcctg
    10621 gggtaagctc gaagaccgag gagagtcacg atgcatgaga tgtccctggc cgagggtgtg
    10681 cgcaccctct tcgaggacgc cgcggcggcc gacggctttg gccacgtgcg cgcggtcgtg
    10741 ctggagatcg gggaactcgc cgcggtcgag gtcgaggcgc tgcgtttctg cctggacgtg
    10801 gtgctggccg acaccatcgc cgccggcgcc gcaatcgagg tcgaaaccct gcccggcgcc
    10861 ggctggtgcc ccgcttgcgc gcaagaggtg ccgatccacc agctctacga cccctgcccg
    10921 cactgcggcg gctaccgcgt gcagaccact gccggccggg agatgcgcgt gaaggcgctg
    10981 gaggtggact gaagatgggc cggcgctcga aggccagacg cggctccggt cacgcaaggc
    11041 ggcgccccgc ccggaccccc gatgtttaca cggggctcca gtacgcgatg gttgcaccgg
    11101 gctccggtgc gcgatggttt tttgtaggag cgacgcaagt cgcgatccag ccgcacgggc
    11161 aagcgcctgc gcccgccccc tcgccgcaat cgcgacttgc gtcgctcctg cgtccacctg
    11221 aacggccgcg gtcgggtcag gggcgtgatc cttacaggca gcaaggagaa acgagagatg
    11281 tgcacgacat gtggttgcgg cagcggtgaa acccggatcg gcgcgctcgg tggcgggcgc
    11341 cccgcgggtg gcgcacaccc gcggcccggc gcgacgcgca catggc
//
LOCUS       DAOQNN010000054        11358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2621_length_11358_cov_11.573034, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000054 DAOQNN010000000
VERSION     DAOQNN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2621_length_11358_cov_11.573034"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="PKV97_05105"
     CDS             <1..1310
                     /locus_tag="PKV97_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNB05290.1"
                     /translation="VVEHDEDTIRRADHVIDLGPGAGVRGGRVVAEGTIADLLAAPES
                     ATGECFRHPLQHPLRPRRAVALENPAIRIEGANLHNLRDIDVRIPLARLTVVTGVSGS
                     GKSSLARDVIHANLRARLGDPEATRARRRGQAQPEAVDQPLVGCAAMPGWHQVGRVLE
                     VDQTPIGKTPRSCPATYVGFWDAIRKLFADTFDARTRGWNASRFSFNTGAGRCPVCDG
                     AGQTTVEMSFLPDVKTPCEACGGARFNPETLSVRWKDRTVAEVLAMPVDEAVDFFSAH
                     PAIAHPLQLLQDVGLGYLTLGQPSPTLSGGEAQRIKLVTELAKVRRRPGDAEDTGGVP
                     LPADKHSLYVLDEPTVGLHMADVDKLIRVLHRLTDAGHTVLVIEHDLDVMAEADWLID
                     LGPEGGEGGGRVVAEGPPEVAMATPESHTGRHLREFLAQRKTE"
     gene            1379..2059
                     /locus_tag="PKV97_05110"
     CDS             1379..2059
                     /locus_tag="PKV97_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNB05291.1"
                     /translation="MRKVRVWDLPTRLFHWLLAILVVAAYVTAQIGGSLIEWHGRVGI
                     AITGLLAFRLVWGVIGSTYARFADFVPGPAHLWAHLRGHWSGLGHNPLGALSVLALLG
                     VMLYQAVTGLFSNDEIAFSGPLRALVSSETSDWLSGLHRQNYWAIIVLVGLHVSAVLF
                     YALVKKDNLLRPMVTGMKEVDEADAQPAQGGGALAFVIALAITAAVLYFATGSFIEPP
                     PPPPPAAW"
     gene            complement(2115..7751)
                     /locus_tag="PKV97_05115"
     CDS             complement(2115..7751)
                     /locus_tag="PKV97_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNB05292.1"
                     /translation="MSNQPKVTIVRIPGRVRPDEPLRLASWIGGDSPLFAGVAPARSF
                     RLGATGSRGTAEDTVAEAIPEDALVEIEFEGGACFWTVGADLRKRLQEAGGASARGGS
                     GADDGAWDLPSSIAAPVTGTRGLAGKLTIRALRWMGFDPVKAGLDKIVDLAETRSVPR
                     EGVFLVGDDGIRGEPLKTPIPDGERTLILLHGTASNTRGSFSKLWLRQPKQWARLRAC
                     YGERIFALEHCTLTRSPIENALKLARLLPRSGSLRLLSHSRGGLVGELLALDPARVDR
                     AGITAVLAAAQRENPPAAEALMQQEAQLFALLRELEARPALQIERFVRVACPVIGTSL
                     AGEKLDQWFSLLVLAAIADEMRRSNSRGMITSGLASLASHGLDMLAETAAAIIGSRKR
                     ADQLPGLEAQMPGSALVRILNGNPGRGELFVVAGDAAPREQDWLAELRFCLIDRYFEQ
                     DHDLVVDTRAMLDGPDRPVRKVFFRAGPTVNHFSYFGNEDSAAAVAAALTDAVDTATL
                     FHDYAGEWPLPAETARGLFAPRALADPPLQGARGIVVLVPGLCGSELQVAGREVWASV
                     PALACGGFRKTLRIDNPAVEPGDPLAGSYLALANALRSSGYVVRMHGYDWRKSVVDSI
                     KGLGDTLSTALGDATAGKLPVHAIVHSMGGVLIRAAFAQDRGLWKRWKEQPRPEARVI
                     MLGTPNQGSHAVTLLLTKRDEFFAKLAMLDLHNSERELLEIAQHFPGVLELLPNAADE
                     NGEEIRLPATWQRYCTADGQRWPVPPEAALTQSGELWRTLLAGDPLIDDERLIYVAGT
                     AKETPRLAEIAEDGRFRLLKTADGDGRVTWASGIPAKCREGGRLYYMNAVHGDMPAHT
                     PAFPALQELLEKGETGRSALTRKPVPIQARGAVEAPGEYAPVSPAMLSHYPTRSDLLA
                     AATGATLRAPRTEREEEMRTRVRVIHGDLIFVDAPVLVGHIQGTNNLEQAEYILDRAL
                     NGRMQNRLDAGIYAGPLGSYALFPPQPEERFAAGAIVIGIGRVGELSPGSLCDGVARA
                     LVAYAITRHEERKRTHDRQAATAGKPLQLPVCALLIGAAIGELSLRDSVTAILRGHRK
                     ARERLADAKLDDRIELTQLDFVELLEDRAIQALNAARDAVTLDGELRRHFCIEDTLAK
                     NEGGRQRAYAELGEDAWTRIAISAVRIADGSGTQLLFNVHGDLARTEQLYNGIALGSI
                     ERFTRQLIDTESDNEEVGRTLFELLLPNWLKDQAPDRRRAQLIIDSAVAGIPWELLRD
                     RMEDDRSDRGGAPLSVRSGLVRQLSTGRFRQRIQRADGFHALVVGDPDLGRLGDYFPQ
                     LAGAREEAAEVAQLLKDGGFDTPPALLGGSAEQIQIALYQRDWRILHLSGHGIYRETL
                     PTIDADHSAKPADEAVTATGMLIGENSVLTTAHIEQMRVVPDFVFINCCSLGRIEAER
                     AMLGENRPGNPMLAANLATQLIEMGVRGVIAAGWRVLDDAAKLFASTFYEAFLAGETF
                     GEAVLAARRATFDRFPTTNTWGAYQCYGDPSLLLRRPPSSHERRRHAARYDFVAPREV
                     IAELERLTQQSRYQAMESADDRKRREERIDALARLCERRGWSGHGDIQEAFGQLWAEF
                     GDHDRAIACYRAAQGAGDGSASMKATEQLINMLARRGSGLLAAGGPEAMAKGKTLLGE
                     AEQLITGVLAVAPNAERHCLLGSIWKRKLKLTADNQLDAALKSMAQAYRNAENCALEA
                     GGRLYYPALSVLHADLLRQLLGTLTPDEGKAARQRIAAVRKEIEDPAFQARDFWDRVA
                     PLDLALSEALFDTACEENADRQANDASFSKAQEEYQALLAYSSRRERESVVGTLDALK
                     TFLERLPAQRKGAHFARARELVGRLNTLADALRKLL"
     gene            complement(8120..8572)
                     /locus_tag="PKV97_05120"
     CDS             complement(8120..8572)
                     /locus_tag="PKV97_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB05293.1"
                     /translation="MKKAIALGILSLTLATAASAQVKPEDQIKFRQAGYSFMSWNMGK
                     IKANLEGEYNAQQVQAAATAIAGIAGSGMGALYGPGTEKDIGNLKTRVKPEFFQEMPN
                     VGKLATDFNNAAANLAKVAATGDKAAVQKAFADTGSTCKACHDKYRME"
     gene            8844..10466
                     /locus_tag="PKV97_05125"
     CDS             8844..10466
                     /locus_tag="PKV97_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB05294.1"
                     /translation="MIRFSDTPIWLRLTGAIWLMMVAAFGSMIVWETRVNRDMAIEQA
                     KDFAGTVNEMTMAGLTGMMITGTVAQRDVFLDQIKELSVVQDLEVIRGEAVSRVFGAG
                     GARDAARDADERLALVEGRSTMRVDHDAEHGEHLRVVIPSLASPAWLGKDCIACHQVP
                     QGTPLGAVSMRISLDKANAAVNAFRNQSAMFAALVSIPLILFIYLFITRFVTRPLDGL
                     NRGLGRIAEGGGDLRLQLPVRGRDEIGRSARTFNDMMGTLGGLVRHVGDSASAVTEAA
                     SRLATDAEAVAESSQRQNESSMGAARAVDEMVGSISAIAEHAEAVRERSHDSLERSRE
                     GQRSLERLVGEVGEVEAAVRQMADAVVAFVDSTQAISRMTSEVREIAEQTNLLALNAA
                     IEAARAGEQGRGFAVVADEVRKLAEKSARSAGEIDQITREVARRSSSVRGALDDGLGH
                     LASSRQMADAVMQVLTAANSLVVEVGEGLDRIAGATGAQRSASESVTARIGEIAGMAR
                     SNNDALAHTVRSARELGELALRLQESVSRFRF"
     gene            complement(10548..>11358)
                     /locus_tag="PKV97_05130"
     CDS             complement(10548..>11358)
                     /locus_tag="PKV97_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNB05295.1"
                     /translation="YVPFIQTDVAINPGNSGGPLFNLAGEVIGINSQIYSRSGGFMGI
                     SFAIPIDVAMNIKDQLVSHGRVQRGRLGIAIQNVDKDLAQSFGLTDPRGALVASVEPG
                     SAADKAGLQAGDVVLAVDGRRIDDSAELPRVIGEKRPGTRVKLELWRDGRSREVAATL
                     DELKAETVAGSAPAPSQVERIGEQFGLSARALTAEETARLQLRGGVVVEGADGAAARA
                     GLQRGDVILAINNQPVASVADLRAQLERAGKRFALLIQRGEARIFVPVRLE"
BASE COUNT         1616 a         3875 c         4078 g         1789 t
ORIGIN      
        1 tggtggtgga gcacgacgag gacaccatcc gccgcgccga ccacgtcatc gacctcggcc
       61 ccggcgccgg cgtgcgcggc ggccgggtgg tggccgaggg tacgatcgcc gacctgctgg
      121 cggcgcccga atcggccacc ggcgaatgct tccgccaccc gctgcagcac ccgctgcgcc
      181 cgcgccgcgc ggtggcgctg gagaacccgg cgatccgcat cgagggcgcc aacctgcaca
      241 acctgcgcga catcgacgtg cgcatcccgc tcgcccgcct cacggtggtg accggcgtgt
      301 ccggctcggg caagtccagc ctggcgcgcg acgtcatcca cgccaacctg cgcgcccgcc
      361 tcggcgaccc cgaggcgacc cgcgcccgcc gccgcggcca ggcgcagccg gaggcggtcg
      421 accagcccct ggtcggctgc gcggcgatgc ccggctggca ccaggtcggc cgcgtgctgg
      481 aggtcgacca gacgccgatc ggcaagacgc cgcgctcctg cccggcgacc tacgtcggct
      541 tctgggacgc gatccgcaag ctcttcgccg acaccttcga cgcccgcacg cgcggctgga
      601 acgcctcgcg cttctccttc aacaccggcg ccgggcgctg cccggtgtgc gatggcgcgg
      661 gccagaccac cgtcgagatg agcttcctgc ccgacgtcaa gacgccgtgc gaggcctgcg
      721 gcggcgcgcg cttcaacccc gagacgctgt cggtgcgctg gaaggacagg acggtggccg
      781 aggtgctggc gatgccggtg gacgaggcgg tggatttctt ctccgcgcat cccgcgatcg
      841 cccacccgct gcagctgctg caggacgtcg gcctcggcta cctgaccctg ggccagccca
      901 gcccgacgct gtcgggcggc gaggcgcagc gcatcaagct ggtgaccgag ctcgccaagg
      961 tgcgccgccg cccgggcgat gcggaagaca ccggcggcgt gccgctgccc gccgacaagc
     1021 acagcctgta cgtgctcgac gagcccaccg tgggcctgca catggccgac gtggacaagc
     1081 tgatccgggt gctgcaccgc ctgacggatg ccggccacac cgtgctggtg atcgagcacg
     1141 acctcgacgt gatggccgag gccgactggc tgatcgacct cggcccggag ggcggcgagg
     1201 gcggtggccg ggtggtggca gaaggcccgc ccgaggtcgc gatggcgacg ccggaatccc
     1261 acaccggccg ccatctgcgc gagttcctgg cgcagcgcaa gacggaatga tgcagtgcag
     1321 cggaacctgt gccggatggc gcagtcagtc aggctgaatc tcagggaagg aatacagcat
     1381 gcgcaaggtc agagtctggg atctgcccac tcgtcttttt cactggctgc tcgccatcct
     1441 ggtggtggcc gcctacgtca ccgcccagat cggcggcagc ctcatcgaat ggcacggccg
     1501 cgtcggcatc gccatcaccg ggctgctcgc cttccgcctg gtgtgggggg tgatcggctc
     1561 gacctacgcc cgcttcgccg acttcgtgcc cggcccggcc cacctgtggg cgcacctgcg
     1621 cgggcactgg agcgggctgg ggcacaaccc gctcggcgcc ttgtcggtgc ttgccctgct
     1681 cggggtcatg ctctatcagg ccgtcaccgg gctgttttcc aatgacgaga tcgccttctc
     1741 cggtccgctg cgcgcgctgg tgagcagcga gaccagcgac tggctgtccg ggctgcaccg
     1801 gcagaactac tgggcgatca tcgtgctggt gggcctgcac gtgtcggccg tgctgttcta
     1861 cgcgctggtc aagaaggaca acctgctgcg gccgatggtc accggcatga aggaagtcga
     1921 cgaagccgac gcccagcccg cgcagggcgg cggcgcgctg gccttcgtga ttgcgctggc
     1981 gatcaccgcc gcggtgctct acttcgccac cggcagcttc atcgagccgc ctccgcctcc
     2041 gccgcccgcc gcctggtgag gggcgacagc gcgcaccgac ggacggtggg tggtcggtgc
     2101 gcgccagaca tgcctcacag caactttcgc aaggcgtcgg ccagggtgtt cagtcgaccg
     2161 accagctccc tcgcgcgggc gaaatgagcg ccttttcgct gcgctggcag gcgttccagg
     2221 aaggtcttga gcgcgtccag cgtaccgacg accgactccc gttcgcggcg tgagctgtag
     2281 gcgagcaagg cctggtactc ctcctgcgcc ttgctgaagc tcgcatcatt cgcctggcga
     2341 tccgcatttt cttcgcaggc ggtgtcgaac aaggcttcgc tcagggccag gtcgaggggt
     2401 gcgacacgat cccagaaatc gcgcgcctgg aatgccgggt cctcgatctc cttgcgcacg
     2461 gcggcgatgc gttgccgtgc cgccttgccc tcgtccgggg tgagggtgcc gagcagctgg
     2521 cgcagcaggt ccgcatggag gacgctgagc gccgggtagt agagccgccc gcccgcctcg
     2581 agggcacagt tttcggcgtt gcggtaggcc tgtgccatgc tcttcagcgc tgcatccagc
     2641 tggttgtcgg cggtgagttt cagcttgcgc ttccagatgc tgccgagcag gcagtgacgt
     2701 tcggcattcg gagcgacggc gagcactccg gtgatgagct gctcggcctc gccgagcagg
     2761 gtcttgccct ttgccatggc ctcgggcccg cccgccgcga gcaggccgct gccccgtcgc
     2821 gcgagcatgt ttatgagttg ctcggtggcc ttcatcgagg cgctgccgtc cccggccccc
     2881 tgggctgccc ggtagcaggc gatggcgcga tcatggtcgc cgaattccgc ccacagctgc
     2941 ccgaaggcct cctggatgtc gccatgcccc gaccatccgc gccgctcgca cagcctcgcg
     3001 agcgcgtcga tgcgctcctc gcgacgcttg cggtcgtcgg cggattccat ggcctggtag
     3061 cgactctgct gggtcaggcg ctccagttcg gcgatcacct cccgcggtgc gacgaagtcg
     3121 tagcgtgcgg catggcggcg gcgctcgtgg ctgcttggcg gccgccgcag cagcaggctg
     3181 gggtcgccgt agcattggta ggcgccccag gtgttggtgg tggggaagcg gtcgaaagtg
     3241 gcccggcggg cggcgagcac cgcctcgccg aaggtctcgc cggcaaggaa ggcttcgtag
     3301 aaggtgctgg cgaacagctt cgccgcgtcg tccagtaccc gccatccggc ggcgatgacg
     3361 ccacggacgc ccatctcgat caactgggtc gcgaggttgg cggcgagcat cggattgccg
     3421 ggccggttct cgccgagcat cgcgcgctcg gcctcgatgc gaccgaggga gcagcagttg
     3481 atgaacacga aatcgggtac cacccgcatc tgctcgatgt gcgcggtggt gagcacgctg
     3541 ttctcgccga tcagcatgcc ggtcgcagtg acggcctcgt cggccggctt ggcgctgtgg
     3601 tcggcatcga tcgtcggcag ggtttcacga tagatgccat ggcccgacag gtgcaggatg
     3661 cgccagtcgc gctggtagag cgcgatctgg atctgctcgg cactgccacc gagcagcgcg
     3721 ggcggcgtgt cgaagccgcc gtccttgagc agctgggcca cctcggcggc ttcttcgcgc
     3781 gcaccggcca gctgcggaaa gtagtcgccc agacgcccga ggtcgggatc gcccacgacc
     3841 agcgcgtgaa agccgtccgc gcgctggatg cgttggcgga agcggcccgt ggagagttgg
     3901 cgtacgaggc cgctgcgcac cgacagcggg gcgccgccgc gatcgctgcg gtcgtcttcc
     3961 atgcgatcgc gcagcaactc ccagggaatg ccggcgacgg ccgaatcgat gatgagctgg
     4021 gcgcggcggc ggtccggtgc ctggtccttc agccagttgg gcagaagcag ttcgaacagg
     4081 gtgcggccga cttcctcgtt gtcgctttcc gtgtcgatca gctggcgggt gaagcgctcg
     4141 atgctgccga gcgcaatgcc gttgtagagc tgctcggtcc gcgcaaggtc gccatggaca
     4201 ttgaacagca gctgggtgcc gctgccgtcg gcgatcctga cggcgctgat cgcgatccgg
     4261 gtccatgcat cttcgccgag ctcggcgtag gcacgctggc gcccgccctc gttcttcgcc
     4321 agcgtgtcct cgatgcagaa atgcctgcgc agctcgccgt cgagcgtcac cgcgtcgcgt
     4381 gccgcgttga gcgcctggat cgcacggtcc tccagcagct cgacgaaatc cagctgcgtc
     4441 aactcgatgc ggtcgtcgag cttcgcgtcg gcgaggcgtt cgcgcgcctt gcgatggccg
     4501 cgcaggatgg cggtgaccga atcgcgcagg ctcagctcgc cgatcgccgc accgatcaac
     4561 agcgcacaca ccggcaactg cagcggtttg ccggcggtgg cggcctgccg gtcgtgtgtg
     4621 cgcttgcgct cctcgtgccg cgtgatcgca taggcgacca gcgcgcgcgc cacgccgtcg
     4681 cacaggcttc ccggcgagag ctcgccgacg cggccgatgc cgatgacgat ggcgccggcg
     4741 gcgaagcgct cctcgggctg gggtgggaag agggcatagc tgcccagcgg gcccgcatag
     4801 atgccggcat ccaggcggtt ctgcatgcga ccgttgaggg cgcggtcgag gatgtattcc
     4861 gcctgctcga gattgttggt gccctgtatg tggccgacca gaaccggtgc gtcgacgaag
     4921 atcaggtcgc cgtgaatgac ccggacccgc gtccgcatct cctcctcgcg ctcggtgcgg
     4981 ggggcgcgga gcgtggcgcc ggtcgccgcg gccagcaggt cggagcgcgt cgggtagtgt
     5041 gaaagcatgg ccgggctgac gggggcatat tccccggggg cttcgacggc gccgcgggcc
     5101 tggatgggga cgggcttgcg cgtcagcgcg gagcgcccgg tctcgccctt ctccagcagc
     5161 tcctgcaatg ccgggaaggc cggcgtgtgc gccggcatgt cgccatgcac cgcgttcatg
     5221 taatagagcc gtccgccttc ccggcacttc gcggggattc cgcttgccca ggtgacgcgg
     5281 ccatcgccgt cggccgtctt gagcaggcgg aaccgtccgt cctcggcgat ctcggcgagg
     5341 cggggcgttt ccttcgccgt gccggcgaca tagatcaggc gctcgtcgtc gatcagggga
     5401 tcgcctgcga gcagcgtccg ccacagttct ccggactggg tgagcgcagc ctccggcgga
     5461 acgggccagc gttggccgtc ggcggtgcag tagcgctgcc aggtcgcggg caggcggatc
     5521 tcctcgccgt tttcatcggc ggcgttcggc agcagctcga gcacaccggg gaagtgctgg
     5581 gcgatctcga gaagctcgcg ctcggagttg tgcaggtcga gcatcgccag cttggcgaag
     5641 aactcgtcgc gcttggtcag caacagcgtc accgcgtgcg agccctggtt gggcgtgccg
     5701 agcatgatga cgcgcgcctc ggggcgcggc tgttccttcc agcgcttcca gagcccgcgg
     5761 tcctgggcga aggcggcgcg gatcagcacg ccgcccatcg aatggacgat cgcgtgcacc
     5821 ggcagcttgc ccgccgtggc atcgcccagc gcggtggaca gggtgtcgcc gagccccttg
     5881 atcgagtcga cgacggactt gcgccagtcg tagccatgca tgcgtacgac atagccgctg
     5941 ctgcgcagcg cattggccag cgcgaggtag ctgccggcca gcggatcgcc cggctcgacc
     6001 gccggattgt cgatgcgcag cgtcttgcgg aagccgccgc aggccagggc cggcacgctc
     6061 gcccacactt cgcggccggc gacctggagt tcggagccgc acaggccggg tacgagcacc
     6121 acgatgcctc gcgcgccctg gagcgggggg tcggcgagcg cacgcggggc gaagaggccg
     6181 cgcgcggtct cggcgggcag gggccattcc cctgcgtagt cgtggaacag cgtggcggtg
     6241 tcgacggcgt cggtcagcgc ggcggcgacc gcggctgcgc tgtcctcgtt accgaagtag
     6301 ctgaagtgat tgacggtcgg gccggcgcgg aagaacacct tgcgtaccgg gcggtcgggg
     6361 ccgtccagca tggcgcgcgt gtcgaccacg aggtcgtggt cctgctcgaa gtagcggtcg
     6421 atcaggcaga agcgcagctc ggccagccag tcctgttccc ggggggcggc gtcgcccgcg
     6481 acgacgaaca gttctccgcg tcccgggttg ccgttgagga tgcggaccag cgcggagccg
     6541 ggcatctgcg cctcgaggcc gggcaactgg tcggcccgct tgcggctgcc gatgatggcc
     6601 gccgcggttt ccgccagcat gtccagccca tggctggcga gggaggcgag cccgctcgtg
     6661 atcatgccgc ggctgttgct gcggcgcatc tcgtcggcga tcgcggccag caccagcagc
     6721 gagaaccact ggtcgagctt ctccccggcg agggaggtgc cgatgaccgg gcaggccacg
     6781 cgcacgaaac gctcgatctg cagcgccggg cgcgcctcga gttcgcgcag cagcgcgaag
     6841 agctgtgcct cctgctgcat cagcgcctcc gccgcaggcg gattctcccg ctgcgccgca
     6901 gccagcaccg cggtgatgcc ggcgcgatcg acgcgagccg gatccagcgc gagcagttcg
     6961 ccgaccaggc caccacggga atgcgacagg aggcgcagcg aaccgctgcg tggcagcagg
     7021 cgcgcgagct tgagcgcgtt ctcgatcggg ctgcgggtca gggtgcagtg ctcgagcgcg
     7081 aagatgcgct cgccgtagca tgcgcgcagg cgcgcccact gtttgggctg gcgcagccac
     7141 agcttggaga agctgccgcg cgtgttcgat gccgtgccgt gaagcaggat gagggtgcgc
     7201 tcgccatcgg gaatcggcgt tttcaggggt tcgccccgga tgccgtcgtc gcccacgaga
     7261 aacacccctt cgcgcggcac cgagcgtgtc tcggccaggt cgacgatctt gtccagaccc
     7321 gccttcaccg gatcgaagcc catccagcgc agggcccgga tggtcagttt cccggcgagc
     7381 ccgcgcgtac ccgtgaccgg cgcggcgatg ctggacggca gatcccaggc gccatcgtcc
     7441 gcgccgcttc caccccgtgc gctggcgccg cccgcctcct gcaggcgctt gcgcagatcc
     7501 gcgcccacgg tccagaaaca tgcgccgcct tcgaactcga tctcgaccag ggcgtcctcg
     7561 ggaatcgcct cggcgaccgt gtcttcggcc gtgccgcgac tgccggtcgc gccgaggcga
     7621 aaggaacgcg ccggcgccac accggcgaac aagggcgaat cacccccgat ccagctcgca
     7681 agccgaagcg gttcgtcggg ccgcacccga cccggtatgc ggacgatggt gactttgggc
     7741 tgattgctca tggaatgggc tcccgctccg atcttcaagc gttgaacaga tgctcgtcgt
     7801 cggccgctgc cacggcagcc cgcgcgcgca ggccgcgggt cgaggcagat acccgggtgc
     7861 ggcgtgtgca gtccctgaag agaagcaccc gcctcgaccc atggcgacga atcctgattc
     7921 acatatagac gatgcgggta gcgttgtccg ctgtgaatcc gcggcgtatt tgcagccggc
     7981 attcggggat gttcgatggc ggggagtgtg gcgatgccgc cggcctgccg gcggcggctc
     8041 gatgccggcc taggcgcgca tccgtgccag gaacgacaaa gccacccgca ggtggctttg
     8101 tcgttcgata tctgacgcct cactccatcc ggtatttgtc gtgacaggcc ttgcaggtgc
     8161 tgccggtgtc ggcgaaggcc ttctgcaccg cggccttgtc gccggtggcg gcgaccttgg
     8221 cgaggttggc ggcggcgttg ttgaagtcgg tggcgagctt gccgacgttc ggcatctcct
     8281 ggaagaactc cggcttgacg cgggtcttga ggttgccgat gtccttctcg gtgcccgggc
     8341 cgtagagcgc gcccatgccc gagccggcga tgccggcgat cgcggtggcg gcggcctgga
     8401 cctgctgggc gttgtactcg ccctcgaggt tagccttgat cttgcccatg ttccagctca
     8461 tgaagctgta gccggcctgg cggaacttga tttggtcctc gggcttgacc tgggccgaag
     8521 ccgcggtggc gagggtaagg gagaggatgc cgagggcgat cgcttttttc atcgtgagcc
     8581 tcctgagtcg ggggttgaag gaaggaacaa accttgcggg gaactgcgca gaccaggatg
     8641 cgccgcgcca gttccatcgt gttgcatgcc gggatccgcc tgccggcacc cgacttataa
     8701 caaattcggt cgatattcct tcgtccgctc gtgttgcggc gcggaagaag tgcgctcaag
     8761 ccgggccgtg cgcggccgta aggaaggagt agaggctgcg tgtccccacg ccgccgaccg
     8821 tccgcaggac cgcgagaacc gacatgatcc gattttccga caccccgata tggctgcgtc
     8881 tcaccggcgc gatctggctg atgatggtgg ccgcgttcgg cagcatgatc gtctgggaaa
     8941 cccgggtgaa ccgcgacatg gcgatcgagc aggcgaagga cttcgccggc acggtcaatg
     9001 agatgaccat ggccggactc accggcatga tgatcaccgg cacggtggcg cagcgcgacg
     9061 tcttcctcga ccagatcaag gagctcagcg tggtgcagga cctcgaggtg atccgcggcg
     9121 aggcggtgag ccgggtgttc ggggcgggcg gtgcgcgcga cgcggcgcgc gacgccgacg
     9181 agcgcctggc cctggtcgag ggccgctcca caatgcgggt cgaccacgac gccgagcatg
     9241 gcgagcacct gcgcgtggtg attccctcgc tggcctcgcc cgcctggctc ggcaaggact
     9301 gcatcgcctg ccaccaagtg ccacagggca cgccgctcgg cgcggtgtcg atgcgcatct
     9361 cgctcgacaa ggcgaatgcc gcggtgaatg cgttccgcaa ccagagcgcg atgttcgccg
     9421 cgctggtgtc gatcccgctg atcctgttca tctacctctt catcacccgc ttcgtcaccc
     9481 gtccgctcga cggcctcaac cggggcctgg ggcggatcgc cgagggtggc ggcgacctgc
     9541 gcctgcaact gccggtgcgc ggccgcgacg agatcggccg cagcgcgcgc accttcaacg
     9601 acatgatggg cacgctcggc gggctggtgc gccacgtcgg cgactcggca agcgcggtga
     9661 ccgaggcggc gagccggctc gccaccgatg ccgaggcggt tgccgagagc tcgcagcgcc
     9721 agaacgagag ctcgatgggc gcggctcgcg cggtggacga gatggtcggc agcatctccg
     9781 cgatcgccga gcacgccgag gcggtgcgcg agcgctcgca cgacagcctg gaacgctcgc
     9841 gcgagggcca gcgcagcctg gagcgtctgg tcggcgaggt cggcgaggtc gaggccgcgg
     9901 tgcgccagat ggccgacgcg gtggtcgcct tcgtcgactc cacccaggcg atctcgcgca
     9961 tgaccagcga ggtgcgcgag atcgccgagc agaccaacct gctcgcgctc aacgctgcga
    10021 tcgaggccgc gcgcgccggc gagcagggcc gcggcttcgc ggtggtggcc gacgaggtgc
    10081 gcaagctggc ggagaaatcc gcgcgctcgg cgggcgagat cgaccagatc acccgcgagg
    10141 tggcccggcg ttcgagctcg gtgcgcggcg cgctcgacga cggcctcggt cacctcgcct
    10201 ccagccggca gatggccgac gcggtgatgc aggtgctgac cgcggcgaat tcgctcgtgg
    10261 tcgaggtggg cgaaggcctc gaccgcatcg ccggcgccac gggtgcgcag cgcagtgcga
    10321 gcgagtcggt caccgcgcgc atcggcgaga tcgccggcat ggcgcgcagc aacaacgacg
    10381 cgctcgccca caccgtgcgt tcggcgcgcg agctcggcga actcgccctg cgcctgcagg
    10441 aatccgtgtc gcgcttccgt ttctgagtgc gcccaggcgg acgtggaaag ggcgggaacc
    10501 gtcgccggtc ccgccctctt gcccgctcgc ccttcagcgc gctgccatca ctccaggcgt
    10561 accggcacga agatgcgtgc ctcgccacgc tggatcagca gcgcgaagcg cttgccggcg
    10621 cgctcgagct gggcgcgcag gtctgcgacg ctggcgaccg gctggttgtt gatcgccagg
    10681 atgacgtcgc cgcgctgcag gccggcgcgg gccgcggcgc cgtcggcgcc ctccaccacc
    10741 accccgccgc gcagctgcag gcgggcggtc tcttccgcgg tgagcgcacg cgcgctcagt
    10801 ccgaactgct cgccgatacg ctccacctgg ctgggcgccg gtgcgctgcc ggccacggtc
    10861 tcggccttga gctcgtccag cgtggcggcg acctcgcggc tgcggccgtc gcgccacagc
    10921 tccagcttca cgcgcgtgcc cgggcgcttc tcgccgatca cccgcggcag ctcggcagag
    10981 tcgtcgatgc ggcggccatc gaccgcgagc accacgtcgc ccgcctgcag cccggccttg
    11041 tcggcggcgc tgcccggctc gacgctggcg accagcgcgc cgcgcggatc ggtgaggccg
    11101 aaggattggg cgaggtcctt gtcgacgttc tggatggcga tgccgaggcg gccgcgctgc
    11161 acgcggccgt ggctgacgag ctggtccttg atgttcatcg cgacgtcgat cgggatcgcg
    11221 aacgagatcc ccatgaagcc gcccgagcgc gagtagatct gcgagttgat gccgatcacc
    11281 tcgccggcga ggttgaacag cggcccgccg gagttgcccg ggttgatcgc cacgtcggtc
    11341 tggatgaagg gcacgtag
//
LOCUS       DAOQNN010000055        11288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2653_length_11288_cov_10.748509, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000055 DAOQNN010000000
VERSION     DAOQNN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11288
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2653_length_11288_cov_10.748509"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PKV97_05135"
     CDS             complement(<1..732)
                     /locus_tag="PKV97_05135"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNB05296.1"
                     /translation="MSPSTTLDAVADILARAQRILFVTGAGISVDSGLPTYRGIGGLY
                     HDRLTEEGLTIEEALSGEMMATRPEIAWRYIAQIEASCRGAKPNAAHRLIAALEQEKA
                     LVCVLTQNVDGLHRAAGSRNVIEIHGTVHRLHCMSCGQGRRVEDYAGLSIPPECPRCG
                     GILRPDVVLFGEALPELAIERLESVLSQGMDAVVSIGTTSVFPYIAGPVLWAAREGTP
                     TVEINPGDTQVSQFVDHRLRMCAAEA"
     gene            complement(729..2807)
                     /locus_tag="PKV97_05140"
     CDS             complement(729..2807)
                     /locus_tag="PKV97_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HNB05297.1"
                     /translation="MPPSTPSEVRVRAACPHDCPDTCAMEVTVADGRAVRIRGAADMP
                     FTNGALCTKVAHYLERVYSERRLLHPMKRVGRKGEGRFERISWDEALDTIAAKYREIA
                     AEDPRAILPYSYCGTMGLVQGESLDRRFFHRLGASLLERTICATAGAHGWKATVGAML
                     GADPEAASEAGLILIWGANPVVSNLHGWRHLQEAKRRGARLVCIDPRRTDTAKKCDLH
                     LAPLPGTDGALALAMMQVLIAEGLLDREYIARHTLGFDALAVRVRACTPEWAAPLTGL
                     AAEAIRSLAIEYGRSARSLIRLNYGLNRCAGGGMAVRNVACLPALTGAWRHPGCGALL
                     STSGNFPVDVQALARPDLYPDARRFPPRALNMSFIGDVLLDSRDPPVRAIHVYNANPV
                     AVAPDSRRVRAGFAREDLFCVVHEQFQTDTADYADILLPATSQLEHRDLHKAYGHLYV
                     VDNRPAIAPLGEARSNTAVFRALARRLGFAAAALYESDDAIAAAAFRADDPRVAGLAE
                     GLQARGWARLALPAGHAPFADGGFRTPSGKCEFFSAALAHEGLDPLPAWHPPAESVPS
                     NPALGARYPLALITPPARNFLNSTFANQARFLADEGGPKLEIHPEDAVARGVVEGMRL
                     RIHNDRGDFIARAVVTTDVRPGVVCSPSVWWPKLCEGGHNANAVIAARPTDMGGGPTF
                     YDCLVEVEPA"
     gene            complement(2855..3529)
                     /locus_tag="PKV97_05145"
     CDS             complement(2855..3529)
                     /locus_tag="PKV97_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05298.1"
                     /translation="MGFPRAERVLAGFACTVALVASSGTLAAQQSGGASIYCCDVGGQ
                     PVCGDILPAACYGRAYREMSPSGTVRRTVAAPLTPDEIAQRAAAEHKRRAEEAERMRQ
                     LRLDQALLETYRSVQDLDSRRDRELRELDRSIRELRARESELIERQRVMIDEATHTDK
                     SDVAASLEADIRLLDSEIAAQSSVVAAKLRERNAVVERFEEHRRRYVELTSAPESARI
                     PPMPRR"
     gene            complement(3510..4154)
                     /gene="pyrE"
                     /locus_tag="PKV97_05150"
     CDS             complement(3510..4154)
                     /gene="pyrE"
                     /locus_tag="PKV97_05150"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNB05299.1"
                     /translation="MDFSRDFIALACRKGVLRFGAFVTKAGRNSPYFFNAGLFDDGAS
                     FRELCGYYARTIRAAGIACDMLFGPAYKGIPLVAGTAIRLAEDGVDLPFAFNRKEAKD
                     HGEGGTLIGAPLRGKVLILDDVISAGTSVRESVELIRAAGAEPAGVVIALDRMERGKS
                     ALSAVEEVKENFGIPVVAVATLEDLVAYLADSPELAANLDAVQAYREQYGISSR"
     gene            4194..4976
                     /locus_tag="PKV97_05155"
     CDS             4194..4976
                     /locus_tag="PKV97_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNB05300.1"
                     /translation="MLRIITLNLNGIRSACTKGLIDWLPAQQADFLCVQELKAQDADL
                     RPEMRAPAGMQGWFHAAEKKGYSGVGIYSRHAPDRVVEGLGIPAIDAEGRFLQLDFGK
                     LSVVSLYLPSGSSSAERQQVKFDFMDRFLPHMDQLFQSGQEVVVCGDWNIAHREADLK
                     NWKSNQKNSGFLPEERAWLGRLFDEQGWIDVYRRLHPEATADCYTWWSNRGQAWAKNV
                     GWRLDYQVATPGLAASAQAAEVYKAQRFSDHAPLTVDYDWTL"
     gene            5122..5604
                     /locus_tag="PKV97_05160"
     CDS             5122..5604
                     /locus_tag="PKV97_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05301.1"
                     /translation="MYNPDRRRFIARLGTGGLLLTPLAACSGEVWPEGMKPIAWDRDA
                     CVRCNMRIADHRFAAQLRGGPRDNTFTFDDVGCLVFWLEEQRTRFPWMDDVSTRMWVA
                     AWESPSVKAVTWLDPRQARYIVKRSPMRYDLAASAAPAADAHSLSYEEMRRKTLARGK
                     "
     gene            5618..5956
                     /locus_tag="PKV97_05165"
     CDS             5618..5956
                     /locus_tag="PKV97_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05302.1"
                     /translation="MPLERPSDRVLAATLLAGVLFAATFADAARQRSAREAALQRTTE
                     LVTGLRLSDVVLFTEARYTRHLSQADLHSAFQDHPMALEHFPSGSLLPPPRLLLDHHE
                     TLDRKAASPD"
     gene            5919..7055
                     /locus_tag="PKV97_05170"
     CDS             5919..7055
                     /locus_tag="PKV97_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNB05303.1"
                     /translation="MKPWIERQRHLIDFTAQSLARRKGKSLGLLFVYTLLVFVLASVA
                     LYTHALRNEATRVLAGAPEIVLQRLIAGRHDLVPPGYIERIGRIRGVQKIEGRLWGYY
                     YDSVLKANYTFMVPADREIVPGEIVVGPALERTRGLSAGNGISFRAYSGELHTFIVAA
                     VLAHESELVSADLVLMNEADFRRFFAYPDGHYTDIALWVANPLEVRNVGVKLLGALPD
                     SRPILREEVLRTYASIFDWREGMMLALLSAAILAFGIFAWEKAAGLSAEEKREIGILK
                     AIGWETGDVIAMKFWEGFLVSLFAFLVGYVAAYVHVFHFEFTLFAPVLEGWAVLYPSF
                     ALTPQIDGLQVATLFVFTVLPYTAATLVPIWRAATTDPDTVMRS"
     gene            7060..7758
                     /locus_tag="PKV97_05175"
     CDS             7060..7758
                     /locus_tag="PKV97_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB05304.1"
                     /translation="MLELANIRKVFNQGQHNEYWALKGIDLEIPPARVSVLKGPSGSG
                     KTTLLTILGCLGRPTEGRVRLNGEDISGLPERFLTEIRRRTFGFIFQQFNLIRGLSAV
                     ENIILPGYPGGTPRAQLLARAEALLADLQLAHRRDAKVEWLSGGEQQRVAICRALIND
                     PEVLIADEPTANLDSTLSAEFLAILRRLAEAGRTVILTSHDPLVVESEVVDRVYSLRD
                     GRLIEVVDTRKAWP"
     gene            7755..8765
                     /locus_tag="PKV97_05180"
     CDS             7755..8765
                     /locus_tag="PKV97_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05305.1"
                     /translation="MIFQPAIIALLLAAGLSVAMLAVASPFALQLIRHWDLGSGSERQ
                     LRLERLTYLFSMMVALVCGLQVLSTLLFVFNADRMSVMFVGAMCAVGTLNANAYGFPA
                     LYAQLVAFFFAATWLAINRLDNQARDYPLTRIKYRLLLAYAPLAAAVLYLQFNYFIRL
                     EADVITSCCGSLFSEEADTVASEVSALAPARAMSVFYATLGGAILLAAWHWKSDGHGL
                     WSGLAVGTASVAAFFAALAGVVAFVSLYIYEHPHHHCPFCILKPDYGYQGYLLYIPLF
                     AAAAAGLAAGVLRMFGGRPSLATLAPQASARLAGIAAAGFVLFMLVASVMVLRSNLIL
                     LD"
     gene            8777..9280
                     /locus_tag="PKV97_05185"
     CDS             8777..9280
                     /locus_tag="PKV97_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB05306.1"
                     /translation="MKNLLLVLACTLLAACGRGGSDAGPARGIAAPAFEAMGLDGGVR
                     RFPEDFADKPVIIDFWADWCRYCANSMTRIDRAWREHAASGLSVLAVNVAQDRATAAA
                     FIERLGVGYSAVLDPGARITRDYGVKGLPMTIFVDRNGLISGKVIGELDDRLLAIQLD
                     RILPETR"
     gene            9374..9985
                     /locus_tag="PKV97_05190"
     CDS             9374..9985
                     /locus_tag="PKV97_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNB05307.1"
                     /translation="MDRREALKMSVLASIGMATAGKAAAQSGCTGDGTPAQFIPKTAA
                     DPQPQVDDLAKHPKCPYCGMDRKQFHHSRMVVHYADDLADGTCSLHCAAISLALNIDR
                     DPKALWVGDNAAEGDTKPLVEVEKASFLVGSKLAGVMTARSKVAYGSAEAAKVSQAAN
                     GGELTDFDGALLAAYTDMSADVTRIRKNRAERRQKMMEQRNKG"
     gene            9982..10470
                     /locus_tag="PKV97_05195"
     CDS             9982..10470
                     /locus_tag="PKV97_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNB05308.1"
                     /translation="MKTLLAFVFAAWTGFALAQAPAAELPKPGTKDLCPVCGMLVSKY
                     PNWVATIVYKDGHAHFFDGAKDLFKFWHDPAKYAAGHSRDQIERISVTEYYGLTAIDA
                     RNAWYVVGSDVLGPMGHEFVPLASREDAADFLKEHKGARVLRFDEVGAGLPSMLDAGR
                     FE"
     gene            complement(10565..11251)
                     /locus_tag="PKV97_05200"
     CDS             complement(10565..11251)
                     /locus_tag="PKV97_05200"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB05309.1"
                     /translation="MLKPLQGYAESKRDVRLRRVFGGAPGAVAWQNRGIFRERAWRRP
                     ERLRMKTLQGQASHRQGCTMIRKFAPILCLGLLATTVHAAEDIDTAALFSASLTGLDG
                     QPVAMERFRGRPLIVNFWARWCAPCREEIPELIRLRDAQGADGIEVIGVALESQVEGV
                     REFATANDMRYPVVLAAKQGIPLMKALGNAAGGVPYTVYIGRDGKAVGSKLGIVRADD
                     LAAATKVLLK"
BASE COUNT         1697 a         3968 c         3913 g         1710 t
ORIGIN      
        1 ggcctcggcg gcgcacatgc gcaggcggtg gtcgacgaac tggctgacct gggtgtcgcc
       61 ggggttgatc tccaccgtgg gtgtgccctc gcgcgccgcc cacagcaccg ggccggcgat
      121 gtaggggaag acgctggtgg tgccgatcga caccaccgcg tccatgccct gcgacagcac
      181 gctctccagg cgctcgatcg cgagctcggg cagcgcctcg ccgaacagca ccacgtccgg
      241 gcgcaggatg ccgccgcaac gcgggcattc gggcgggatg gagaggccgg cgtagtcctc
      301 cacccggcgc ccctgcccgc aggacatgca gtgcaggcga tgcacggtgc cgtggatctc
      361 gatgacgttg cgcgaaccgg cggcgcggtg caggccatcg acgttctggg tgagcacgca
      421 caccagcgcc ttctcctgct cgagtgcggc gatcaggcgg tgggcggcgt tgggcttcgc
      481 accgcggcag ctggcctcga tctgggcgat gtagcgccag gcgatctcgg gccgggtcgc
      541 catcatctcg cccgacaagg cctcctcgat ggtgaggccc tcctcggtga ggcggtcgtg
      601 gtagaggccg ccgatgccgc ggtaggtggg aagccccgaa tcgaccgaga tgccggcgcc
      661 ggtgacgaag aggatgcgct gcgcgcgcgc gaggatgtcc gccaccgcgt cgagggtcgt
      721 gctcgggctc atgcgggctc cacctccacc aggcagtcgt agaaggtcgg cccgccgccc
      781 atgtcggtgg ggcgcgcggc gatgaccgcg ttggcgttgt ggccgccttc gcacagtttg
      841 ggccaccaca ccgaggggct gcacaccacg ccggggcgca cgtcggtggt gaccaccgca
      901 cgcgcgatga agtcaccgcg gtcgttgtgg atgcgcaggc gcatgccctc cacgacgccg
      961 cgcgcgacgg cgtcctcggg gtggatctcg agcttcggcc cgccctcgtc ggcgagaaag
     1021 cgcgcctggt tggcgaaggt ggaattgagg aagttgcgcg ccggcggggt gatcagcgcg
     1081 agcggatagc gcgcgcccag cgccgggttg ctgggcaccg actcggcggg cggatgccag
     1141 gccggcaaag ggtcgaggcc ctcgtgcgcg agcgcggcgg agaagaactc gcacttgccc
     1201 gagggcgtgc ggaagccacc gtcggcgaag ggcgcgtggc ccgcgggcag cgcgagccgc
     1261 gcccagcccc gcgcctgcag gccctcggcg aggcccgcca cccgcgggtc gtcggcgcgg
     1321 aaggccgccg ccgcgatggc gtcgtcgctt tcgtagagcg cggcagccgc aaagccgagg
     1381 cggcgggcga gcgcacggaa gaccgcggtg ttggagcgcg cctcgccgag cggcgcgatc
     1441 gccggccggt tgtccacaac gtagagatgg ccgtaggcct tgtgcaggtc acggtgctcg
     1501 agctggctgg tggcgggcag caggatgtcg gcgtagtcgg cggtgtcggt ctgaaactgc
     1561 tcgtgcacca cacagaagag gtcctcgcgc gcaaagccgg cgcgcacccg acggctgtcg
     1621 ggcgcgactg cgaccgggtt ggcgttatag acgtggatcg cgcgcaccgg cgggtcgcgg
     1681 ctgtcgagca gcacgtcgcc gatgaaggac atgttgagcg cccgcggcgg gaagcgcctc
     1741 gcgtcgggat agaggtcggg gcgggcgagc gcctgcacat ccaccgggaa gttgcccgag
     1801 gtcgacagca gcgcgccgca gccggggtgg cgccacgcgc cggtgagcgc gggcaggcag
     1861 gccacgttgc gcaccgccat gccgccaccg gcgcagcggt tgaggccgta gttgagccgg
     1921 atcaggctgc gcgcgctgcg cccgtactcg atcgccaggc tgcggatggc ctcggccgcg
     1981 aggccggtga gcggcgccgc ccactccggc gtgcaggcgc gcacgcgtac ggcgagcgcg
     2041 tcgaagccca gggtgtggcg ggcgatgtac tcgcggtcga gcaggccctc ggcgatcagc
     2101 acctgcatca tcgcgagcgc gagcgcgccg tcggtgccgg gcagcggggc gaggtgcagg
     2161 tcgcacttct tcgcggtatc ggtgcgccgc ggatcgatgc acaccaggcg ggcgccgcgc
     2221 cgcttcgcct cctgcaggtg gcgccagccg tgcaggttgg acaccaccgg gttggcgccc
     2281 cagatcagga tcaggccggc ctcgctggcg gcttcgggat cggcgccgag catcgcgccc
     2341 acggtcgcct tccagccgtg cgcgcccgcg gtggcgcaga tcgtgcgctc gagcagggag
     2401 gcgccgaggc gatggaagaa gcgccggtcc aggctctcgc cctgcaccag gcccatggtg
     2461 ccgcagtagc tgtagggcag gatcgcgcgc gggtcctcgg cggcgatctc gcggtatttc
     2521 gccgcgatgg tgtcgagggc ttcgtcccag gagatgcgct cgaagcgccc ctcgcccttg
     2581 cgccccaccc gcttcatcgg atgcagcagg cggcgctcgg aatacacccg ctcgaggtag
     2641 tgcgcgacct tggtgcacag cgcgccgttg gtgaagggca tgtcggcggc gccgcggatc
     2701 ctcaccgcgc ggccatcggc aaccgtgacc tccatcgcgc aggtgtcggg gcagtcgtgc
     2761 gggcaggccg cgcgcacgcg gacttcggag ggggtcgagg ggggcatcgc tgcctcgcgt
     2821 tgcggccgcc cggccgggcg gcacggcgga agactcaacg acgcggcatc ggcgggatgc
     2881 gcgcggactc cggcgccgag gtgagctcga cgtatcggcg acggtgttcc tcgaagcgct
     2941 cgaccaccgc attgcgctcg cgcagcttgg ccgcgaccac cgaggactgg gcggcgatct
     3001 cgctgtcgag caggcggatg tcggcctcga ggctggcggc gacgtcgctc ttgtccgtat
     3061 gcgtggcctc gtcgatcatg acgcgctggc gctcgatcaa ctccgactcg cgtgcgcgca
     3121 gctcgcggat gctgcggtcg agctcgcgca gctcgcgatc gcgccggctg tcgaggtcct
     3181 ggacgctgcg ataggtctcg agcagcgcct ggtcgaggcg cagctggcgc atgcgctcgg
     3241 cctcctcggc gcgacgcttg tgctcggcgg ccgcgcgctg ggcgatctcg tcgggcgtga
     3301 gcggcgccgc gaccgtgcgg cgcacggtgc ccgaggggct catctcgcgg taggcgcggc
     3361 cgtagcaggc ggcgggcagg atgtcgccgc acaccggctg accgccgaca tcgcagcagt
     3421 agatcgatgc gccgccagac tgctgcgccg ccagcgtgcc ggacgaggcc accagcgcga
     3481 cggtgcaggc gaagccggcg aggacgcgtt cagcgcgagg aaatcccata ctgttcccga
     3541 tatgcctgga ctgcgtcgag gttggccgca agctcgggac tgtccgccag gtaggcgacg
     3601 aggtcttcga gcgtggccac cgcgacgacc ggaataccga aattctcctt gacctcctcg
     3661 accgcggaca aggcactttt accgcgttcc atgcgatcga gcgcgatcac cacaccggcg
     3721 ggctcggcgc ccgcggcgcg gatcagctcg accgactcgc gcaccgaggt gccggccgag
     3781 atcacgtcgt cgaggatcag caccttgccg cgcagcggcg cgccgatcag cgtgccgccc
     3841 tcgccgtggt ccttggcctc cttgcggttg aacgcgaagg gcaggtccac gccgtcctcg
     3901 gcgagccgga tcgcggtgcc ggcgacgagc gggatgccct tgtaggccgg gccgaacagc
     3961 atgtcgcagg cgatacccgc ggcgcggatg gtgcgcgcgt agtagccgca cagctcgcgg
     4021 aacgaggcgc cgtcgtcgaa cagaccggca ttgaagaaat acggcgagtt gcgtcccgcc
     4081 ttggtgacga atgcgccgaa gcgcagcacg cccttgcggc acgcgagggc gatgaaatcc
     4141 cggctaaaat ccacggctct ttggctgcct ggcagcgaaa ctggaaaccg ccgatgttac
     4201 gcatcatcac cctgaacctc aacggcatcc gctcggcctg caccaagggc ctgatcgact
     4261 ggctgcccgc gcagcaggcc gacttcctgt gcgtgcagga actcaaggcg caggacgccg
     4321 acctgcgccc cgagatgcgc gcgcccgcgg gcatgcaggg ctggttccac gccgccgaga
     4381 agaagggcta cagcggcgtc ggcatctaca gccggcacgc gccggaccgc gtcgtcgagg
     4441 gcctcggcat ccccgcgatc gacgccgagg gccgcttcct gcagctcgac ttcggcaagc
     4501 tctcggtggt ttcgctctat ctgccctcgg gctcgagctc ggcggagcgc cagcaggtca
     4561 agttcgattt catggaccgc ttcctgccgc acatggacca gctcttccag agcggacagg
     4621 aggtcgtggt gtgcggcgac tggaacatcg cccaccgcga ggccgatctg aagaactgga
     4681 agtcgaacca gaagaactcc ggcttcctgc ccgaggagcg cgcgtggctc gggcgtttgt
     4741 tcgacgaaca gggctggatc gacgtctatc gccgcctgca ccccgaggcc accgcggact
     4801 gctacacgtg gtggtcgaac cgcggccagg cgtgggcgaa gaacgtggga tggcggctgg
     4861 actaccaggt cgccaccccg ggactggctg cgagcgccca ggcggcggag gtctacaagg
     4921 cgcagcgctt ctcggaccac gcgccgctga cggtggatta cgactggacg ctgtagcccc
     4981 gtggggtgcg acacgcggtc gcacccctgc cctcctccgg ggccatgcgg cccgggggcc
     5041 acgcgccggc gctatcatcc gggcattcga acccggcgtc cgcctccaag ggagatcctc
     5101 gagcggtcgg agatgagccc catgtacaac cctgacagac gtcgcttcat cgcccgcctc
     5161 ggcaccggcg gcctgctgct gacgccgctg gcggcctgct cgggagaggt gtggcccgag
     5221 ggcatgaagc ccatcgcctg ggatcgcgat gcctgcgtgc gctgcaacat gcgcatcgcc
     5281 gaccaccgct tcgccgcgca actgcgcggg gggccgaggg acaatacctt cacgttcgac
     5341 gatgtcggct gcctggtctt ctggctcgaa gagcagcgca cgcgctttcc gtggatggac
     5401 gacgtgtcca cccggatgtg ggtggcggcc tgggagagtc cgagcgtcaa ggcggtcacc
     5461 tggctggatc cgcgccaggc ccgctacatc gtcaagcgct cgccgatgcg ctacgacctc
     5521 gctgccagcg cagcgcccgc agccgatgcg cacagcctga gctacgagga aatgcgccgg
     5581 aagaccctgg cgcgcggcaa gtagcgggga ccttggcatg cccttggagc gcccctccga
     5641 ccgcgtcctc gccgccacgc tgctcgccgg cgtgctgttt gcggcaacct tcgccgacgc
     5701 cgccaggcag cgcagcgcac gcgaggcggc actgcagcgc acaacggaac tcgtcaccgg
     5761 cttgcgcctg tccgacgtgg tgctgttcac cgaggcacgc tacacccgcc acctgagcca
     5821 ggccgacctg cactccgcct tccaggacca cccgatggcg ctggagcact tcccctcggg
     5881 ctcgctgctg ccgccaccac gcctgctgct ggaccaccat gaaaccctgg atcgaaaggc
     5941 agcgtcacct gattgacttc accgcgcagt cgctcgcgcg gcgcaagggc aagagcctcg
     6001 ggctgctgtt cgtctatacg ctgctcgtgt tcgtgctcgc ctcggtggcg ctgtacaccc
     6061 acgcgctgcg caacgaggcc acgcgggtgc tggccggcgc gccggagatc gtgctgcagc
     6121 gcctcatcgc cggccgccac gacctggtgc cgccgggcta catcgagcgg atcggccgca
     6181 tccgcggcgt gcagaagatc gagggccggc tgtggggcta ctactacgac agcgtgctga
     6241 aggccaacta caccttcatg gttccggccg atcgcgagat cgtcccgggc gagatcgtcg
     6301 tcggcccggc gctggagcgc acgcgcgggc tgtcggcggg caacggcatc tccttccgcg
     6361 catattccgg cgagttgcac accttcatcg tcgccgcagt gctggcgcac gagtccgaac
     6421 tggtcagcgc cgacctggtg ctgatgaacg aggccgactt ccgccgcttc ttcgcctatc
     6481 cggacggcca ctacaccgac atcgcgctgt gggtggccaa cccgctcgag gtgcgcaacg
     6541 tcggtgtcaa gctgctcggc gccctgcccg attcgcgccc gatcctgcgc gaggaggtgc
     6601 tgcgcaccta tgcgtcgatc ttcgactggc gcgagggcat gatgctggcg ctgctgtcgg
     6661 ccgccatcct cgccttcggc atcttcgcct gggagaaggc tgccggcctg tcggcggaag
     6721 aaaaacgcga gatcggcatc ctcaaggcga tcggctggga gaccggcgac gtgatcgcga
     6781 tgaagttctg ggaaggcttc ctggtctcgc tgttcgcctt cctggtcggc tatgtcgccg
     6841 cctacgtgca tgtgttccac ttcgagttca ccctgttcgc gccggtgctc gagggctggg
     6901 cggtgctgta cccgagcttc gcgctcacgc cgcagatcga cggcctgcag gtggccacgc
     6961 tgttcgtgtt caccgtgctg ccctacaccg cggccaccct ggtgccgatc tggcgcgcgg
     7021 cgaccaccga ccccgacacc gtgatgcgga gctgacgcga tgctcgaact cgccaacatc
     7081 cgcaaggtgt tcaaccaggg ccagcacaac gaatactggg cgctcaaggg catcgacctg
     7141 gagatcccgc cggcgcgggt gagcgtgctc aagggcccct cgggctcggg caagaccacg
     7201 ctgctgacca tcctcggctg cctcggccgc cccaccgagg gccgcgtgcg cctgaacggc
     7261 gaggacatct ccggcctgcc cgagcgcttc ctgaccgaga tccgccgccg caccttcggc
     7321 ttcatcttcc agcagttcaa cctgatccgc ggcctgtcgg cggtcgagaa catcatcctg
     7381 cccggctacc ccggcggcac gccgcgcgcg cagttgctgg cgcgcgccga ggccctgctc
     7441 gccgacctgc agctcgcgca ccgccgcgac gccaaggtgg agtggctctc cgggggcgag
     7501 cagcagcgcg tggcgatctg ccgtgcgctc atcaacgacc ccgaggtcct gatcgccgac
     7561 gaacccaccg ccaacctcga cagcacgctc tcggccgaat tcctcgccat cctgcgccgc
     7621 ctcgccgagg ccgggcgcac ggtgatcctc accagccacg acccgctggt ggtcgagtcc
     7681 gaggtcgtcg accgggtgta ttcgctgcgc gacggccggc tcatcgaggt cgtcgacacg
     7741 cgcaaggcct ggccatgatc ttccagcccg ccatcatcgc cctgctgctc gccgccggcc
     7801 tgagcgtggc catgctggcg gtggcgtcgc cgttcgcgct gcagctcatc cgccactggg
     7861 acctcggcag cggcagcgag cgccagttgc ggctcgagcg cctgacctac ctgttctcga
     7921 tgatggtggc gctggtgtgc gggctgcagg tgctgtcgac gctgctgttc gtgttcaacg
     7981 ccgacaggat gtcggtgatg ttcgtcggcg cgatgtgcgc cgtcggcacg ctcaacgcca
     8041 acgcgtacgg ctttccggcg ctatacgcac agctcgtggc gttcttcttc gcggcgacgt
     8101 ggctggcgat caaccgcctc gacaaccagg cgcgcgacta tccgctcacc cgcatcaagt
     8161 accgcctgct gctcgcctac gcgccgctcg ccgcggcggt gctgtacctg cagttcaact
     8221 acttcatccg cctcgaggcg gacgtcatca cctcgtgctg cggcagcctg ttctcggaag
     8281 aggccgacac ggtcgcgagc gaggtcagcg cgctggcgcc ggcacgggcg atgagcgtct
     8341 tctacgccac gctgggcggc gcgatcctgc tcgctgcctg gcactggaag agcgacggac
     8401 acgggctgtg gagcgggctc gcggtcggca cggcctcggt cgccgccttc ttcgccgcgc
     8461 tcgccggggt ggtcgccttc gtgtcgctgt acatctacga gcatccccat caccactgcc
     8521 cgttctgcat cctgaaaccc gattacggct accagggcta cctgctctac atcccgctgt
     8581 tcgccgccgc cgcggccggg ctggcggctg gggtgctgag gatgttcggc ggccgcccca
     8641 gcctcgccac cctcgccccg caggccagcg cacgcctggc cggcatcgcc gcggcgggct
     8701 ttgtgctctt catgctggtg gcgagcgtga tggtgctgcg ctccaacctg atcctgctcg
     8761 actgaggcca ctgccgatga agaacctgct gctcgtcctt gcctgcaccc tgctcgccgc
     8821 ctgcggccgg ggcggatccg acgccggccc ggcgcgcggc atcgctgccc ccgccttcga
     8881 ggcaatgggc ctggacggcg gcgtacgccg gttcccggaa gactttgccg acaagccggt
     8941 gatcatcgat ttctgggccg actggtgccg ctactgcgcc aacagcatga cgcggatcga
     9001 ccgtgcctgg cgcgaacacg cggcgagcgg actgagcgtg ctggcggtca acgtcgccca
     9061 ggaccgcgcc accgccgccg ccttcatcga gcgcctcggc gtcggttact cggccgtgct
     9121 cgaccccggc gcgcgcatca cccgcgatta cggcgtgaaa ggcctgccga tgaccatctt
     9181 cgtcgatcgc aacgggctga tcagcggcaa ggtcatcggc gaactcgacg acaggctgct
     9241 ggccatccag ctcgatcgca tcctgccgga gacgcgatga cccggccccg ggccgcagcg
     9301 cctgccgcag gcgcagcccg attcgaatcg aaccctgccg gcacgcgccg gcaccacccc
     9361 gaaggagcag accatggatc gcagagaagc cctcaagatg tccgtcctcg ccagcatcgg
     9421 aatggccacc gccggcaagg ccgccgcgca gtccggctgc accggcgacg gcaccccggc
     9481 gcagttcatc cccaagaccg cagccgatcc gcagccgcag gtcgatgacc tcgccaagca
     9541 tcccaaatgc ccgtattgcg gcatggatcg caagcagttc caccactcgc gcatggtcgt
     9601 gcactacgcc gacgacctcg ccgacggcac ctgttcgctg cattgcgcgg cgatcagcct
     9661 ggcgctgaac atcgatcgcg accccaaggc gctgtgggtg ggcgacaacg cagccgaagg
     9721 cgataccaag ccgctggtcg aagtcgagaa ggccagcttc ctggtcggca gcaagttggc
     9781 cggcgtgatg accgcgcgca gcaaggtcgc ctacggcagc gccgaggccg ccaaggtctc
     9841 gcaggcggcg aacggcggcg agctcaccga cttcgacggc gcgctgctgg ccgcctacac
     9901 cgacatgtcg gccgacgtca cccgcatccg caagaaccgc gccgagcgtc gacaaaagat
     9961 gatggagcag cgcaacaaag gatgaagacc ctgctcgctt tcgttttcgc agcctggacg
    10021 gggttcgcgc tcgcgcaggc gccggccgcc gagctgccga agccgggcac gaaggatctg
    10081 tgcccggtgt gcggcatgct ggtgtcgaag tatccgaact gggtggcgac catcgtctac
    10141 aaggacggtc acgcccactt ctttgacggc gccaaggatc tgttcaagtt ctggcacgac
    10201 ccggcgaagt acgctgccgg ccacagccgc gaccagatcg agcgcatttc ggtgaccgag
    10261 tactacgggc tgaccgccat cgacgcgagg aatgcgtggt acgtcgtggg ctcggacgta
    10321 ctggggccga tggggcacga gttcgtgccc ctggccagtc gagaggacgc ggccgacttt
    10381 ctgaaggagc acaagggcgc gcgcgtcctg cgtttcgacg aggtcggcgc gggccttccg
    10441 tcgatgctcg acgccgggcg cttcgagtag cccacggccc gggcgccgat ggcgcccggc
    10501 gccgcccgtg tctgaaagct gcggcaacct ggccgcggca gtgcgggcat tcacccgcac
    10561 gcgctcactt cagcagcacc ttcgtcgccg cggcgagatc gtccgcgcgc acgatcccca
    10621 gcttgctgcc caccgccttg ccgtcgcggc caatatagac cgtgtagggc acgccgcccg
    10681 ccgcgttgcc gagcgccttc atcagcggga tgccctgctt cgccgccagc accaccggat
    10741 agcgcatgtc gttggcggtg gcgaactcgc gcacgccctc gacctggctc tcgagcgcaa
    10801 ccccgatcac ctcgatcccg tccgcgccct gcgcatcgcg caggcggatg agctcgggga
    10861 tctcttcgcg acagggcgcg caccagcgcg cccagaaatt gacgatgagc ggccgcccgc
    10921 ggaagcgttc catggccacc ggctgaccgt cgagcccggt cagcgaagcc gagaacaagg
    10981 ccgcggtgtc gatgtcttcg gcagcgtgga cggtcgtcgc caaaaggccg aggcaaagga
    11041 tcggggcgaa tttacggatc atggtgcaac cttgtcggtg agaagcttgt ccttggagcg
    11101 tcttcatccg cagccgttcc ggtcggcgcc aggcacgttc gcgaaagatg ccccgattct
    11161 gccaagcaac ggcacccggc gcaccgccga aaacacgccg caaccggaca tcgcgcttcg
    11221 attcggcata cccctgcagc ggcttcagca ctcgcttacg catcgttcat tgcggccgga
    11281 cgccccgc
//
LOCUS       DAOQNN010000056        11275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2659_length_11275_cov_11.038235, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000056 DAOQNN010000000
VERSION     DAOQNN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2659_length_11275_cov_11.038235"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1330
                     /locus_tag="PKV97_05205"
     CDS             137..1330
                     /locus_tag="PKV97_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HNB05310.1"
                     /translation="MRALIWLIGIFALAVGVTMLAGVNDGYVLVVLSPWRAQVSLNLF
                     IVVLVVGVALIHLLLRMVTRTVQLPGRVARWRERRRRDKADRALHGSVTALFEGRYSE
                     ALKSASTAYSASDGSPMAALVAARAAYGLQDDTRYREWLDHAAEQGKESEVARLMTEA
                     ELAIDARQFELAAARLAQLRETGQKHIAMLRLESKVAYELGRWEELVNNVRQLRKHKA
                     LTAEQAAPALRRAHVERMRQLVGDASALLGYWQEIPSEELADRGLIKEVLPLMARAGQ
                     AMHMRAQTETLLDEQWDSDLARLYASCANTMDDTNACLHKAEAWLEKQPRDAGLLFAL
                     GQLCRRVELWGKAQSYLEASLSVDPQVGTHLALAHLFETLERRDESQRHYRAAAEKMA
                     VGALA"
     gene            complement(1404..2312)
                     /gene="hemF"
                     /locus_tag="PKV97_05210"
     CDS             complement(1404..2312)
                     /gene="hemF"
                     /locus_tag="PKV97_05210"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNB05311.1"
                     /translation="MDPRSVKPWFTELQRHIVERLEAFDGQAFRSDGWDRPGGGGGLT
                     RVIEDGNFFERGGVNFSHVMGDGMPASATAHRPELAGRRFEAMGVSLVLHPRNPYCPT
                     VHMNVRCFVALAEGKDPVWWFGGGMDLTPYYPFEEDVRHFHRSCKAAVLPWGGEAEYA
                     RLKDWCDRYFFLKHRNEPRGVGGLFFDDLGADGKTGFDTAFGLTKAVGEAFLGGYLPI
                     VERRRDIPYGERERDFQAYRRGRYVEFNLVFDRGTLFGLQSGGRTESILMSMPPVVKW
                     RYDWRPQEGSPEARLYEEFLVPRAWA"
     gene            complement(2446..3726)
                     /gene="purD"
                     /locus_tag="PKV97_05215"
     CDS             complement(2446..3726)
                     /gene="purD"
                     /locus_tag="PKV97_05215"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNB05312.1"
                     /translation="MKVLVIGSGGREHALAWKLAQSPKVTRVLVAPGNPGTAAEKLLQ
                     NVPVTDIKVLVELAKHEHVAFTVVGPEAPLAAGVVDAFRAAGLPIFGPTKAAAQLESS
                     KDFAKQFLVRHNIPTAKYQTFADAAAAHAYVDAEGAPIVIKADGLAAGKGVVVAMTAD
                     EAHAAIDMMLLDNRMGDAGARVVIEEFMEGEEASFIVMADGKHALALATSQDHKRLHD
                     GDQGPNTGGMGAYSPAPVVTPDVHARVMREVINPTLTGMAAEGLPYTGFLYAGLMIDG
                     EGKPRVVEFNCRMGDPETQPIMVRLKTDLADLIEAAIAGKLDQTEAEWDRRFALGVVL
                     AAAGYPESPRKGDVITGLPASEQEDVHVFHAGTALQDSQVVTAGGRVLCVTALGDNVR
                     SAQKRAYEVADGIVFEGRQYRRDIGHRAIGRKTA"
     gene            complement(3761..5350)
                     /gene="purH"
                     /locus_tag="PKV97_05220"
     CDS             complement(3761..5350)
                     /gene="purH"
                     /locus_tag="PKV97_05220"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNB05313.1"
                     /translation="MNVTQALISVSDKRGVLDFSRKLSALGIKLLSTGGTASLLREAG
                     LPVTDVSEHTGFPEMLDGRVKTLHPKVHGGILARRDLAEHMDTIAAHDIGRIDLVVVN
                     LYPFQQTVAKPDCTLEDAIENIDIGGPTMVRAAAKNHGNEQGGVGIVTDPEDYGCIIE
                     ELEANAGKLSHKTRFALAVKAFTHTARYDSAISNYLTALVTNEAGDVSLQTYPERLQL
                     AFDKVQDLRYGENPHQTAAFYRQPGAAEGGVAGYTQLQGKELSYNNIADADAAWECVK
                     AFDGSAAACVIVKHANPCGVAVAASPLEAYKKAFSTDPTSAFGGIIAFNGEVDRAAAE
                     AVSAQFLEVLIAPSYTADALELLASKKNVRVLTCALGQPAGAFDYKRVGGGLLVQSAD
                     EARIQIADLKVVTKRAPTEAEMRDMLFAWRVAKYVKSNAIVYCKDGMTIGVGAGQMSR
                     VDSARIARIKAENAGLQIAGCVVASDAFFPFRDGLDVLAQAGATAVIQPGGSMRDEEV
                     IAAANEQDIAMVFTGFRHFRH"
     gene            complement(5397..5633)
                     /locus_tag="PKV97_05225"
     CDS             complement(5397..5633)
                     /locus_tag="PKV97_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB05314.1"
                     /translation="MSRSNEIADAVVRTLDQYFRDLDGEKPAAIHDMVIRNVERPMLA
                     FVLQQARGNQTVAAEMLGINRNTLRRKLTEYDLL"
     gene            complement(5630..6670)
                     /gene="dusB"
                     /locus_tag="PKV97_05230"
     CDS             complement(5630..6670)
                     /gene="dusB"
                     /locus_tag="PKV97_05230"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNB05315.1"
                     /translation="MQFAGFTLRNNLFVAPMAGVTDRPFRQLCKKLGAGVAVSEMVTS
                     NSLLYGSEKTRRRADHTGEVDPISVQIAGADPAMMAEAAKFNADNGAQIIDINMGCPA
                     KKVCNVMAGSALMQDEPLVARILEAVVKAVPDTPVTLKFRTGWNRANKNAPTIARIAE
                     ESGIRAIAIHGRTRADQYMGEAEYDTIAHIKTLVNIPVIANGDIGSPQKAKHVLDYTG
                     ADGVMIGRAAQGRPWIFREIEHFLATGELLPPPLVSEIHQVCREHLADLYAFYGDDRG
                     VKIARKHISWYTKGLVGSAAFRRAMNQIPDIPAQLAAVDNFFGQLAEAGARLSYEHER
                     APHAEYPQELAA"
     gene            complement(6944..8092)
                     /locus_tag="PKV97_05235"
     CDS             complement(6944..8092)
                     /locus_tag="PKV97_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNB05316.1"
                     /translation="MQHDAKLSPATLAAQGTGATPMPHRDIVPPIHLATTFERAEDGS
                     LPGGRIYARDASPAYDPAEALLCELEGGARAALFASGMAAASAVLLALKPGARIVAPH
                     DMYWSLRNWMIGFAANWQIALDFYADAAELAALLQRPADLVWVETPANPTWEILDIAR
                     AAELAHAAGARLVVDSTVPTPVFTRPLALGADVVMHSATKYLNGHSDVVAGVLVTRAE
                     DDFWARIKTARAAGGAVLGPFEAWLLNRGLRTLFPRVRTAAASALRIAQHFEHHPALL
                     AVLYPGLPSHRGHDIACRQMKGGFGAMLSLRVRGGEDAARRVAGAVKIFRRATSLGSV
                     ESLIEHRGPIEGPGTRCPLDLLRLSVGIEDSDDLIADLEQALQQANEP"
     gene            8245..9273
                     /locus_tag="PKV97_05240"
     CDS             8245..9273
                     /locus_tag="PKV97_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB05317.1"
                     /translation="MIRIENLRKTYRAADGREVEALADIELEIGAGEVFGIIGRSGAG
                     KSTLIRTLNLLERPSAGRVLIDGEDITTLDSEGLYALRRRVGMIFQHFNLLGAKTVAD
                     NIDWPLKATGHTNAAERAVRVKELLALVGLSEQGHKYPSQLSGGQKQRVGIARALANR
                     PQILLCDEATSALDPETTQSILRLLLDINRQLGLTIVLITHEMQVIRTICDRVAVIDG
                     GRIVEAGRVADVFLHPRHPVTRSMVAQSDALAAASFDPAHAYMKDKLRGTLVRLTYIG
                     DVTYQPILSRIMAGAKVQITILQGEVSSIKDVPFGQLLLELEGSEDDIREVFAELDRH
                     QIHHEVLQ"
     gene            9273..9932
                     /locus_tag="PKV97_05245"
     CDS             9273..9932
                     /locus_tag="PKV97_05245"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HNB05318.1"
                     /translation="MDLSVIDWSDIWIATWETLVMTGVSLLFTILLGLPLGILLFVTA
                     RRQLLEQGFVYTVLSFVVNVLRSVPFLILLIVMIPVTVILIGTSLGVEGAIPPLVVGT
                     APFFARLVENVLREVDRGVIEACQAMGIRTSRIIFGALLPEALPGLIAAVTVTAITLM
                     SYAAMSGVIGGGGLGDLAIRFGYQRFQTEVMVITVALLVVLVQIIQYSGDRLVLYFTR
                     K"
     gene            9956..10756
                     /locus_tag="PKV97_05250"
     CDS             9956..10756
                     /locus_tag="PKV97_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNB05319.1"
                     /translation="MSFTLRKLLAAALGTLTLAAGTAAVAADRLVIAATPVPHAEILE
                     FVKPMLAKEGVELEIKVFTDYVQPSMQTNEKRVDGNFFLHQPYLDAFKQKQKNDIQVV
                     VAKVHVEPLGAYSGKHKAVADIPEGATVAIPNDPSNSGRALLLMARNGLITLKDLNNI
                     SATQKDIAGNPKKLKIRELEAATLPRVLNQVDLAVINTNYAIEAKLNPLKDSLFIEDA
                     SSPYANLLVARVDNADSPAIRKLAAALNSPEVKQFLADKYQGAVVPAF"
     gene            complement(10767..>11275)
                     /locus_tag="PKV97_05255"
     CDS             complement(10767..>11275)
                     /locus_tag="PKV97_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HNB05320.1"
                     /translation="GGMLYIWMMSLIFYRYTFFRFSPADLSPPYWINMGAMAISTLAG
                     SQLILNAPHAPFLESLLPFIKGFTVFYWATGSWWIPMLIVLAVWRHGVMRFPLRYDPL
                     YWGAVFPIGMYSACTLRLHEAMALPFLDRIPPVFLAIALCAWALAFVGLAMDLRARVR
                     VPPCASRA"
BASE COUNT         1713 a         3692 c         4061 g         1809 t
ORIGIN      
        1 ccgacatccg cggcccaggg agcggcgacc cagcctgccg ctgccgaggc gcagcccgct
       61 gcgccggccg agcaggcgcc cgcgggttcc gtgaccgacg ctgccgcccc ggcggcgagc
      121 cagtgagggc cgaacgatgc gcgcactgat ctggctgatc ggcatcttcg ccctcgccgt
      181 cggcgtgacg atgctggccg gcgtgaatga cggctacgtt ctcgtcgtgc tgtcgccgtg
      241 gcgggcccag gtttcgctga acctcttcat cgtggtgctg gtggtgggcg tcgccctcat
      301 ccacctgctg ctgcgcatgg tgacccgtac cgtgcagctg cccggccggg tggcgcgctg
      361 gcgcgagcgc cggcgtcgcg acaaggccga ccgggcgctg cacggttcgg tgacggcgct
      421 cttcgagggg cgttactcgg aagccctcaa atccgcctcg accgcctatt cggcgagcga
      481 tggttcgccg atggccgcgc tggtcgcagc gcgcgccgcc tacggcctgc aggacgacac
      541 ccgctaccgc gagtggctgg accacgccgc cgaacagggc aaggagagcg aggtggcccg
      601 cctgatgacc gaggccgagc tcgccatcga tgcccgccag ttcgagctcg cggcggcacg
      661 cctggcgcaa ctgcgcgaga cggggcaaaa gcacatcgcg atgctgcgcc tggagagcaa
      721 ggtcgcgtat gagctcgggc gctgggaaga gctggtgaac aacgtgcgcc agctgcgcaa
      781 gcacaaggcg ttgaccgcgg agcaggctgc gcccgcgctg cgtcgtgctc acgtcgagcg
      841 catgcgccag ctcgtgggtg atgcgtccgc cttgctcggg tactggcagg agattccctc
      901 cgaggagctt gccgaccggg ggctgatcaa ggaggtgctg ccgctgatgg cgcgcgcggg
      961 tcaggcgatg cacatgcgcg cgcagaccga gaccttgctc gacgagcagt gggacagcga
     1021 tctggcacgc ctgtacgcga gctgtgcgaa caccatggac gacaccaacg cttgcctgca
     1081 caaggccgaa gcctggctgg agaagcagcc gcgcgacgcg ggcctgctgt tcgccctcgg
     1141 ccagctttgc cgtcgggtgg agctatgggg caaggcgcag agctatctcg aggcctcgtt
     1201 gagcgtcgat ccgcaggtcg gcacccatct ggcgctcgcg catctcttcg agaccctgga
     1261 gcgccgtgac gagtcccagc gccactaccg cgccgcggcc gagaagatgg cggtcggcgc
     1321 gctggcctga gcgaagaatc acgcagggag atcccggggc cgcgaggccc cgtttttttg
     1381 tccgctgctc gcgatccgac aaatcaggcc caagcgcgcg ggacgaggaa ttcctcgtac
     1441 agcctggcct cgggcgagcc ctcctgcggg cgccagtcgt agcgccactt caccaccggc
     1501 ggcatcgaca tcaggatgga ttcggtgcgt ccgcccgact gcaggccgaa tagcgtgccg
     1561 cggtcgaaga cgagattgaa ctcgacgtag cggccgcgcc ggtaagcctg gaagtcgcgt
     1621 tcgcgctcgc cgtaggggat gtcgcggcgg cgctcgacga tgggcaggta cccacccagg
     1681 aaggcctcgc ccaccgcctt ggtgaggccg aaggcggtgt cgaaaccggt cttgccgtcg
     1741 gcgccgaggt cgtcgaagaa cagcccgccc acgccgcgcg gctcgttgcg gtgcttgagg
     1801 aagaagtagc ggtcgcacca gtccttgagg cgggcgtatt cggcctcgcc gccccagggt
     1861 agcaccgccg ccttgcagct gcgatggaag tggcgcacgt cctcctcgaa ggggtagtag
     1921 ggcgtcaggt ccatgccgcc gccgaaccac cacaccggat ccttgccttc ggccagcgcg
     1981 acgaagcagc gcacgttcat atgcacggtg gggcaatacg gattgcgcgg atgcagcacc
     2041 agcgacacgc ccatggcctc gaagcggcgg ccggcgagct cggggcgatg cgccgtggcc
     2101 gaggcgggca tgccgtctcc catcacgtgc gagaagttaa ccccgccgcg ctcgaagaaa
     2161 ttgccgtcct cgatcactcg ggtgaggccg ccgccaccgc ccgggcggtc ccagccgtcg
     2221 ctgcggaagg cctggccgtc gaaggcctcg aggcgctcga cgatgtggcg ctgcagctcg
     2281 gtgaaccagg gtttgacgct gcgtggatcc atgcgggacc tccgtggcat tggcgcgcca
     2341 gcgccccgcg agagcttcgc gcgcggacgt cggcgctggg tgagagctac gccgtcgctc
     2401 gcggaggcgg agacggcgat ggggcgcctg cacggcgccc ggcgatcagg cggtcttgcg
     2461 gccgatcgcg cggtggccga tgtcgcggcg gtactggcgg ccttcgaaga cgatgccgtc
     2521 ggcgacctcg taggcgcgct tctgcgcgct gcgcacgttg tcgccgagcg cggtcacgca
     2581 cagcacgcgc ccgccggcag tgacgacctg gctgtcctgc agcgcggtgc cggcgtggaa
     2641 gacatgcacg tcctcctgct cggaggcggg cagcccggtg atgacgtcgc ccttgcgcgg
     2701 gctctccggg tagcctgcag cggcgagcac cacgccgagc gcgaagcggc ggtcccactc
     2761 ggcctcggtc tggtcgagct tgccggcgat ggcggcttcg atcagatcgg cgagatcggt
     2821 cttcagacgc accatgatcg gctgcgtctc gggatcgccc atgcgacagt tgaactcgac
     2881 cacgcgcggc ttgccctcgc cgtcgatcat cagcccggcg tacaggaagc cggtgtaggg
     2941 caggccctcg gcggccatgc cggtcagcgt cgggttgatg acctcgcgca tcacgcgcgc
     3001 atgcacgtcg ggggtgacca ccggggcggg cgagtaggcg cccatgccgc cggtgttggg
     3061 gccctggtcg ccgtcgtgca ggcgcttgtg gtcctggctg gtggcgagcg cgagcgcgtg
     3121 cttgccgtcg gccatgacga tgaagctggc ctcctcgccc tccatgaact cctcgatgac
     3181 gacgcgcgca cccgcatcgc ccatgcggtt gtcgagcagc atcatgtcga tcgcggcgtg
     3241 cgcctcgtcg gccgtcatcg ccaccaccac gcccttgccc gcggccaggc cgtcggcctt
     3301 gatcacgatc ggcgcaccct cggcgtcgac gtaggcgtgt gcggcggcgg catccgcgaa
     3361 ggtctggtac ttcgcggtcg ggatgttgtg gcggacgagg aactgcttgg cgaagtcctt
     3421 cgagctctcg agctgggcgg cggccttggt cggaccgaag atcggcaggc cggcggcgcg
     3481 gaaggcatcg accacgccag ccgccagcgg cgcctcgggg ccgaccacgg tgaaggcgac
     3541 gtgctcgtgc ttggcgagct cgaccagcac cttgatgtcg gtgacgggaa cgttctgcag
     3601 cagcttttcg gcagcggtgc cggggttgcc cggcgccacc agcacgcggg tgaccttggg
     3661 cgactgggca agtttccagg ccagcgcgtg ttcgcggccg ccggaaccga tgacgaggac
     3721 tttcatgtgc tgctccgaag ctctgctggg gtggtcgttc ttagtgacgg aagtgacgga
     3781 agccggtgaa caccatggcg atgtcctgct cgttggctgc cgcgatcacc tcttcgtcgc
     3841 gcatcgatcc gccgggctgg atcaccgcgg tcgcacccgc ctgggcgagc acgtcgaggc
     3901 cgtcgcggaa ggggaagaag gcgtccgagg cgaccacgca gccggcgatc tgcagaccgg
     3961 cgttctcggc cttgatcctg gcgatgcgcg ccgagtcgac gcggctcatc tggccggcac
     4021 cgacgccgat ggtcatgccg tccttgcagt acacgatggc gttggacttg acgtacttgg
     4081 ccacgcgcca ggcgaagagc atgtcgcgca tctcggcttc cgtcggcgcc cgcttcgtga
     4141 cgaccttgag gtccgcgatc tggatgcggg cctcgtcggc gctctgcacc agcaggccgc
     4201 caccgacgcg cttgtagtcg aaggcaccgg caggctggcc gagcgcgcag gtgagcacgc
     4261 gcacgttctt cttgctcgcg agcagctcga gcgcgtcggc ggtgtaggac ggcgcgatca
     4321 gcacctcgag gaactgtgcc gaaacggcct cggccgcggc acggtcgacc tcgccgttga
     4381 acgcgatgat gccgccgaag gccgaggtgg ggtcggtgga gaaggccttc ttgtaggcct
     4441 cgagcgggct ggcggcgacg gccacgccac agggattggc gtgcttgacg atgacgcagg
     4501 ccgccgccga gccgtcgaag gccttcacgc attcccacgc cgcgtcggcg tcggcgatgt
     4561 tgttgtagga cagctccttg ccctgcagct gggtgtagcc ggccacgccg ccctcggccg
     4621 cgccgggctg gcggtagaag gccgcggtct ggtgcgggtt ctcgccgtag cgcaggtcct
     4681 gcaccttgtc gaaggcgagc tgcaggcgct cggggtaggt ctgcagcgac acgtctccgg
     4741 cctcgttggt gacgagcgcg gtgaggtagt tggagatcgc cgagtcgtag cgcgcggtgt
     4801 gggtgaaggc cttcaccgcg agcgcgaagc gggtcttgtg gctgagcttg ccggcgttgg
     4861 cctcgagctc ttcgatgatg cagccgtagt cctcggggtc ggtgacgatg ccgacgccgc
     4921 cctgctcgtt gccgtggttc ttggccgcgg cgcgcaccat ggtggggccg ccgatgtcga
     4981 tgttctcgat cgcgtcttcc agcgtgcagt cgggcttggc cacggtctgc tggaaggggt
     5041 agagattgac caccaccagg tcgatgcggc cgatgtcgtg ggcggcgatg gtgtccatgt
     5101 gttcggcgag atcgcggcgg gcgaggatgc cgccatgcac cttcgggtgc agggtcttga
     5161 cccggccgtc cagcatctcg ggaaagccgg tgtgctcgga gacgtcggtc accggcaggc
     5221 cggcctcgcg cagcaggctg gcggtgccgc cggtcgacag cagcttgatg ccgagcgcgg
     5281 agagcttgcg ggagaagtcg agcacgccac gtttgtcgga gacgctgatc agggcttggg
     5341 tcacgttcat cgttgggccg gaagcggaag gatggaggaa aaggggaagc acccgctcag
     5401 agcaggtcgt attcagtcag cttgcgccgc agggtgttgc ggttgatgcc gagcatctcg
     5461 gcggccacgg tctggttgcc cctggcctgc tggaggacga aggccagcat cggacgctcg
     5521 acgttgcgga tcaccatgtc gtggatcgcc gccggcttct cgccgtcgag gtcgcggaaa
     5581 tactggtcga gggtgcggac gacggcgtcc gcgatctcgt tgctgcggct catgctgcga
     5641 gttcctgcgg atattcggcg tgcggcgcgc gctcgtgctc gtagctcagg cgggcgccgg
     5701 cctcggcgag ctggccgaag aagttgtcca ccgcggcgag ctgggcggga atgtcgggga
     5761 tctggttcat cgcgcggcgg aaggccgccg agcccaccag gcccttggtg taccaggaga
     5821 tgtgcttgcg cgcgatcttg acgccgcggt cgtcgccgta gaaggcgtag aggtcggcga
     5881 ggtgctcgcg gcacacttgg tggatctcgc tcaccagcgg cggcggcagg agctcgccgg
     5941 tggcgaggaa atgctcgatc tcgcggaaga tccacggccg gccctgcgcc gcgcgcccga
     6001 tcatgacgcc gtcggcgccg gtgtagtcga gcacgtgctt ggctttctgc gggctgccga
     6061 tgtcgccgtt ggcgatcaca gggatgttca cgagggtctt gatgtgggcg atggtgtcgt
     6121 actccgcctc gcccatgtac tggtcggccc gggtacggcc gtggatggcg atggcgcgga
     6181 tgccggactc ctcggcgatg cgcgcgatcg tcggcgcgtt cttgttggcg cggttccagc
     6241 cggtgcggaa cttgagcgtg accggggtat cgggcaccgc cttgactacg gcctcgagga
     6301 tgcgcgcgac cagcggctcg tcctgcatca gcgccgagcc ggccatcacg ttgcacacct
     6361 tcttggccgg gcagcccatg ttgatgtcga tgatctgcgc gccgttgtcg gcgttgaact
     6421 tcgcggcctc ggccatcatc gccgggtcgg cgccggcgat ctgcaccgag atcggatcga
     6481 cctcgccggt atgatcggcg cggcggcggg tcttctcgct gccgtagagc agcgaattgg
     6541 aggtgaccat ctcggatacg gcgacgcctg cgcccagctt tttgcagagc tggcggaagg
     6601 ggcggtccgt cacgccggcc atgggggcga cgaagaggtt gttgcgcagg gtgaagccgg
     6661 caaactgcat ggttggaagg acgaggggaa acgcagcatt ttaccctatc gatcctgggc
     6721 gtgcgcttgt tggggcgagt gtgcaacacg ctgatgctgc gcagctccct gcaggcgcct
     6781 gtcgggcgca cgacggatgg tgcgtcgggc gtggcgcgct ctgcgtgttg tggtgtgcgc
     6841 ggaggcaggg ggcggtgagt gcgaaagggc aactgctgca gtgcgcggcg cggggttgcg
     6901 gggccggggt gggggcggct tgcggacgaa acccgctcgg gcttcagggc tcgtttgctt
     6961 gctgcagcgc ctgttcgagg tcggcgatca ggtcgtcgct atcttcgatg cccaccgaga
     7021 ggcgcagcag gtcgagcggg cagcgcgtgc ccggaccctc gatcgggccg cggtgctcga
     7081 tcaggctctc gaccgagccg agcgaggtcg cgcggcggaa gatcttcacc gcgccggcga
     7141 cccggcgtgc ggcatcctcg ccgcctctca ctcgcagcga cagcatggcg ccgaagccgc
     7201 ctttcatctg gcggcaggcg atgtcgtggc cgcggtgcga gggcagcccg gggtagagca
     7261 cggcgagcag cgccgggtga tgttcgaaat gctgcgcgat gcgcagcgcc gaggcggccg
     7321 cggtgcgcac gcggggaaag agcgtgcgca ggccgcggtt gagcagccag gcttcgaacg
     7381 ggccgagcac cgcgccgccg gcggcccgtg cggtcttgat gcgcgcccag aagtcgtcct
     7441 cggcgcgcgt caccagaacg ccggcgacca cgtccgagtg gccgttcaga tatttggtcg
     7501 ccgagtgcat caccacgtcg gccccgagcg ccagcggccg ggtgaagacc ggtgtcggca
     7561 cggtggagtc caccaccagg cgcgcgcccg cggcgtgggc gagttcggcg gcgcgcgcga
     7621 tgtcgaggat ttcccaggtc gggtttgcgg gcgtctccac ccacaccagg tcggccgggc
     7681 gctgcagcag tgcggcgagc tccgcggcgt cggcgtagaa atcgagcgcg atctgccagt
     7741 tggcggcaaa gccgatcatc cagttgcgca gcgaccagta catgtcgtgc ggtgcgacga
     7801 tgcgcgcgcc cggcttcagc gccagcagca ccgccgaggc cgcggccatg ccggaggcga
     7861 acagcgccgc tcgagcgccg ccttccagct cgcacagcag ggcctcggcg gggtcgtagg
     7921 ccgggctggc gtcgcgcgcg tagatgcgcc cgccgggcag gctgccgtcc tcggcgcgct
     7981 cgaaggtggt cgccagatgg atcggcggca cgatgtcgcg gtgcggcatc ggggtggcgc
     8041 ccgtgccctg ggcggcgagg gtggcggggg agagcttggc gtcgtgctgc atgatgcgcg
     8101 gtccttcggg cgggggatgc ggtgaggatg agaaggaggg cgggcggctg cccgggtggc
     8161 ggcgagccta caagttcccc gtagtggtgt cgagtccggg ctgcgggtaa gatctcaggc
     8221 ttttttccag tccggaaagc tcccgtgatc cgcatcgaaa acctgcgcaa gacctaccgt
     8281 gccgccgacg gccgcgaggt ggaggcgctc gccgacatcg agctcgagat cggcgccggc
     8341 gaagtgttcg gcatcatcgg ccgctcgggc gcgggcaaga gcacgctgat ccgtaccctc
     8401 aacctgctcg aacgcccctc ggccgggcga gtgctgatcg acggcgagga catcaccacg
     8461 ctcgacagcg agggcctgta cgcgctgcgt cggcgcgtcg gcatgatttt ccagcacttc
     8521 aacctgctcg gcgccaagac cgtcgccgac aacatcgact ggcccctgaa ggcgaccggg
     8581 cacacgaatg ccgccgagcg tgcggtgagg gtgaaggagc tgctcgcgct ggtcggactg
     8641 tccgagcaag gccacaagta tccgtcgcag ctctccggcg ggcagaagca gcgcgtgggc
     8701 atcgcgcgcg cgctcgccaa ccggccgcag atcctgctgt gcgacgaggc gacctcggcg
     8761 ctcgaccccg agaccacgca gtccatcctg cgcctgctgc tcgacatcaa ccgccagctc
     8821 gggctcacca tcgtgctgat cacccacgag atgcaggtca tccgcaccat ctgcgaccgt
     8881 gtcgcggtga tcgacggcgg gcgcatcgtc gaggccggca gggtggccga cgtcttcctg
     8941 cacccgcggc atccggtcac gcgcagcatg gtggcgcaga gcgacgcgct cgcggcggcg
     9001 agcttcgatc cggcgcacgc ctacatgaag gacaagctgc gcggcacgct ggtgcgcctg
     9061 acctacatcg gcgacgtcac ctaccagccc atcctgagcc gcatcatggc gggcgcgaag
     9121 gtgcagatca cgatcctgca gggcgaggtc tcgagcatca aggacgtccc cttcggccag
     9181 ctgctgctcg agctcgaggg cagcgaggac gacatccgcg aggtgttcgc cgagctcgac
     9241 cgccaccaga tccaccacga ggtgctgcag taatggacct gtccgtcatc gactggagcg
     9301 acatctggat cgcgacctgg gagaccctgg tcatgaccgg ggtgtcgctg ctcttcacca
     9361 tcctgctcgg cctgccgctg ggcatcctgc tgttcgtcac cgccaggcgc cagctgctgg
     9421 agcagggctt cgtctatacg gtgctgtcct tcgtggtcaa cgtgctgcgc tcggtgccct
     9481 tcctgatcct cttgatcgtg atgatcccgg tcacggtgat cctgatcggc acctcgctcg
     9541 gggtcgaggg cgcgatcccg ccgctggtgg tgggcaccgc gcccttcttc gcgcgcctgg
     9601 tcgagaacgt gctgcgcgag gtcgaccgcg gcgtgatcga ggcctgccag gcgatgggca
     9661 tccgcacctc gcgcatcatc ttcggcgcgc tgctgcccga ggccctgccc gggctgatcg
     9721 ccgccgtcac cgtgacggcg atcaccttga tgtcctatgc tgcgatgtcc ggcgtgatcg
     9781 gcggcggcgg cctcggcgac ctcgcgatcc gtttcggcta tcagcgcttc cagaccgagg
     9841 tgatggtgat caccgtcgcc ctgctggtcg tgctcgtcca gatcatccag tactcgggcg
     9901 accgcctggt gctgtatttc acccgcaagt gatccccacg aaggagactt caacgatgtc
     9961 gtttaccctg cgcaaactcc tggcggccgc cctcggcacg ctcaccctgg ccgccggcac
    10021 cgccgccgtg gccgccgacc gcctggtcat cgccgccacc ccggtgccgc acgccgagat
    10081 cctcgagttc gtcaagccga tgctggccaa ggagggcgtg gaactcgaga tcaaggtgtt
    10141 caccgactac gtccagccct cgatgcagac caacgagaag cgcgtcgacg gcaacttctt
    10201 cctgcaccag ccctacctcg acgcgttcaa gcagaagcag aagaacgaca tccaggtcgt
    10261 ggtcgccaag gtgcacgtcg agccgctcgg cgcctactcg ggcaagcaca aggcggtggc
    10321 ggacattccc gagggcgcga cggtcgcgat cccgaacgac ccgtccaact ccggccgcgc
    10381 gctgctgctg atggccagga acggcctgat cacgctgaag gatctgaaca acatctcggc
    10441 cacccagaag gacatcgccg gcaacccgaa gaagctgaag atccgcgaac tcgaggccgc
    10501 gaccctgccg cgcgtgctga accaggtcga cctcgcggtc atcaacacca actacgcgat
    10561 cgaggccaag ctcaacccgc tgaaggactc cctcttcatc gaggatgcga gctcgcccta
    10621 cgccaacctg ctggtggcgc gcgtagacaa tgccgacagt ccggcgatca ggaagctcgc
    10681 cgccgcgctg aattcgcccg aggtgaagca gttccttgcc gacaagtacc agggcgcggt
    10741 ggtgccggcg ttctgatcgt ctcgcatcag gcccgcgatg cgcatggtgg tacgcgcacg
    10801 cgggcgcgca ggtccatcgc caggccgacg aaggccagtg cccaggcgca cagcgcgatg
    10861 gccaggaata cgggcggaat gcggtcgagg aagggcaggg ccatcgcctc gtgcaggcgc
    10921 agggtgcagg ccgagtacat cccgatcggg aacaccgcac cccagtacag cgggtcgtag
    10981 cgcagcggga agcgcatcac gccgtggcgc cacacggcga gcacgatcag catcgggatc
    11041 caccagctcc cggtcgccca gtagaacacc gtgaacccct tgatgaaggg cagcagcgat
    11101 tcgaggaagg gcgcgtgcgg ggcgttcagg atcagctggc tgccggccag cgtcgagatc
    11161 gccatcgcac ccatgttgat ccagtagggc ggcgacaggt cggcgggcga gaaacggaaa
    11221 aaggtgtagc ggtagaagat cagcgacatc atccagatgt acagcatgcc gcccc
//
LOCUS       DAOQNN010000057        11238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2672_length_11238_cov_11.916570, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000057 DAOQNN010000000
VERSION     DAOQNN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2672_length_11238_cov_11.916570"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PKV97_05260"
     CDS             complement(<1..412)
                     /locus_tag="PKV97_05260"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNB05321.1"
                     /translation="MKKIVIAPDSYKESLSALEVAQAIAAGFRQVFPVAEYVLVPVAD
                     GGEGTVDAMVAATGGRKEIVTVSGPLGQPVEAFYGLTGDGATAVIEMAAASGLALVPP
                     DRRNPLVTSSRGTGELIRAALDAGARRFILGIGGS"
     gene            complement(537..1679)
                     /locus_tag="PKV97_05265"
     CDS             complement(537..1679)
                     /locus_tag="PKV97_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HNB05322.1"
                     /translation="MFLLDASLAQSIVDRAMQILRTNVNVMDERGLIIASGDRARIGS
                     RHEGALLVLAQGRAVEVDAAVAQRLHDTRPGINLPLRAEGRVVGVVGLSGEPDAVRQQ
                     AELVRLAAETMLEQAQWMQMLARDQRLREELVLQLIGVSPPGAGLDDWASRLGVDLAR
                     PCVALLIEIADASLAADAVLAAQQRLRRLLAEQEPGLACAPVTLRELAVLMPLPAAES
                     DLRSRLQALHGRLGGEGSGQPCLALGGRFGGDDGLARSWQSARATLQAGRRHRPQAAI
                     HCYDELTLPVLLAGLGEGWRADELRRPLWRLAQQDRDGQLRRTLAAWFAHDMKAAPSA
                     GALHVHRNTLDYRLRRIGEITGLDLARLNDCLLLFIGLELDVAENS"
     assembly_gap    1760..1859
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1860..3071
                     /locus_tag="PKV97_05270"
     CDS             <1860..3071
                     /locus_tag="PKV97_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNB05323.1"
                     /translation="ARSGELPAEIARLIEQAADEILAGRHDAQFPLVVWQTGSGTQTN
                     MNVNEVVAGRANELAGQGRGGKAPVHPNDHVNRAQSSNDCFPTAMHLAAARAVHDELL
                     PAITELSNGLAIQAERHAGVLKTGRTHLMDATPITFGQELSAFVAQLNTAAHAIRAAL
                     PAVCELAQGGTAVGTGLNAPPGFGVEITAELAALTGLPLVSAPDKFAALAGHEPLVAL
                     SGALKTLAVALMKLANDLRLLGSGPRAGLAELKLPANEPGSSIMPGKVNPTQCEALSM
                     IACQVLGNDATIAFAASQGHLQLNVFKPVIAYNLLQSIRLLADGCRNFQRHCVAGLEA
                     DHARMSEHLERSLMLVTALNPHIGYDKAAQIAKKAWAEGTTLRTAALALGFTSAEEFD
                     AWVRPQDMLGR"
     gene            3308..3526
                     /locus_tag="PKV97_05275"
     CDS             3308..3526
                     /locus_tag="PKV97_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNB05324.1"
                     /translation="MKIRTLVPALLLAAAATGCATWDNLSSREKSAATGAAVGGVAGA
                     VITDGGILGTVGGAAIGGVIGDQVGKKK"
     gene            3663..5270
                     /locus_tag="PKV97_05280"
     CDS             3663..5270
                     /locus_tag="PKV97_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNB05325.1"
                     /translation="MTTTPFPPELLRDVEKRRTFAIISHPDAGKTTLTEKLLLFGGAI
                     QLAGTVKARKSARHATSDWMEVEKQRGISVTSSVMQFEYQGHTINLLDTPGHEDFSED
                     TYRVLTAVDAAVMVIDAAKGVEAQTIKLLEVCRLRNTPIITFVNKLDREVREPFELLA
                     EIEDVLKITCAPVTWPLGMGKAFRGVYHLLQHQVLTFTPGEERRSDGEIIKGLDNPEL
                     DKRFPMEVGQLREDVELIDGASPPFDLDDFLAGKQSPVFFGSGINNFGVQEILQSLID
                     WAPPPQARDGGSRMVQPAEPAFTGFVFKIQANMDPKHRDRIAFFRICSGRYSAGMKVR
                     HVRAGREMKLANVLTFMANERVIMEDGVAGDIVGIHNHGQLQIGDTLTEGESLGFKGI
                     PYFSPEMFRAARLRDPLKSKQLQKGLQELGEEGAIQVFELEGGQMLLGAVGVLQFEIV
                     AQRLKDEYKVDAIFEPADIHTARWLTFPDDVTRRNFEREQALRLGKDIDGNPVYLATS
                     RYNLAVTAEKWPKVTFHATREHGEVLG"
     gene            complement(5331..6221)
                     /locus_tag="PKV97_05285"
     CDS             complement(5331..6221)
                     /locus_tag="PKV97_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB05326.1"
                     /translation="MKEHQLRALVQVAECSSIRAAARSMHLSQSALTKSLRELEEDVG
                     EELMTRSYKGIEFTPAGSMLLSHARLALSILDKAAREIRLLRGGAGARVVLAVTPMAG
                     AVALPRVLREYEKLCPDAEVHLTEGLLSQVLPDLIEGRLDFAIAVADPAELPYEVIFE
                     PLGPANAVLEGREGHPLAAATTWAELRDAKFVMNLSPGSLGRALMSWMEQQGLPTPRH
                     VIHCSSTLLMLELMQRTDCIGIGPGRLFDDKFVGYGLQRIDVTPMPPPMCFGLLRLRA
                     LPLSTAAKPMATLFSRYLST"
     gene            6418..7887
                     /locus_tag="PKV97_05290"
     CDS             6418..7887
                     /locus_tag="PKV97_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB05327.1"
                     /translation="MTAVVGTPSTAYQGNDKLLYGIILGVITFWLFAQTVLNIAPDMQ
                     KDLGLDASMMNNAVAITALFSGIFIVVIGGLADRIGRVKTVQIGFLLSIVGSLLIALA
                     PTGSLATTFLLVGRALQGVSAACIMPASLALVKTYWDGPARQRAVSLWSIGSWGGSGV
                     CSLFGGLVAENLGWRYIFFASIVVAVIGMLMVKGTPESKAETKGAYKFDLPGVLTFMI
                     SMVALQIVVTQGNKMGWTSPLIMGLIAVVVIFGTLFFRIENTSGQPFVDFKLFQNPTY
                     TGATLSNFLLNGVAGTLLVSLQLVQLGGNMTAQQAGGLTLGYAIAIIAFIRVGEKLLQ
                     RFGARKPMIWGCLIVGVSIVMLSPANLLLEDYNTLAIIGYTLFGVGLAFYATPSTDAA
                     LSALPAAQAGSGAGIYKMASSLGAAFGVAISAAIFTALSANPESIAWLEGVITFAGRQ
                     DNLAVREAAIIALMFNVFMVVVAIMSIMLTVPKFVGKKN"
     gene            8032..9189
                     /locus_tag="PKV97_05295"
     CDS             8032..9189
                     /locus_tag="PKV97_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HNB05328.1"
                     /translation="MSTLLQELSANQEAMKTWRHHMHRHPEVAFDEHKTASYIAGLLR
                     EWGYEVTEGVGKTGVVARMRCGDSKKSIGLRADTDALAVQEANDSDHKSTIEGKSHTC
                     GHDGHSAMLLGAAQHLARTRAFNGTVNLIFQPAEEIMGGAVAMIQDGLFERFPMDAVF
                     GMHNMPALEQGKMYFTSGPLFAAADNWEIVLTGVGSHGSMPEKSIDPVVAGASLVMAL
                     QTIVSRNVAAKDSAVVSVGAFLAGDAGNVIPQTATLRLSTRTSKPETRQLVLAKIKSI
                     TAAQAEAFGVRYEIREGSPGAVLVNDPALTEECADVARKLLGEDQVVMPGPTYMGSED
                     FAFFAQLKPSTYCIIGNGDTAMVHHPKYDFDDDNLPVGAAYWVALTQHHLK"
     gene            complement(9465..9665)
                     /locus_tag="PKV97_05300"
     CDS             complement(9465..9665)
                     /locus_tag="PKV97_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05329.1"
                     /translation="MTENARAAEPIAGWALWRLDDNGNRFLVAVFACRDEAEAAVRAF
                     EAHGHKQTYWVEAAGDRGCPPV"
     gene            complement(9669..10190)
                     /gene="hemG"
                     /locus_tag="PKV97_05305"
     CDS             complement(9669..10190)
                     /gene="hemG"
                     /locus_tag="PKV97_05305"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HNB05330.1"
                     /translation="MRLLIVYSTIDGHTLEICERIAAIARATGHEVELAEVESASAEQ
                     LEACDRIVIGASIRYGHHRPAVKAFIDRHQAALEVRPNAFFSVNAVGRKPHKRQADTN
                     PYVRKFLGQLRWKPQQVEVFGGKIEYPRYGFFDRNMIRLIMWITGGPTDPRSVTDFTD
                     WTQVEAFAHQLLA"
     gene            complement(10211..10591)
                     /locus_tag="PKV97_05310"
     CDS             complement(10211..10591)
                     /locus_tag="PKV97_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05331.1"
                     /translation="MRKYSKPVRSLIAAVALVTVSTGALAQNFMKDKAEVEKLLTGAT
                     LIGVYLRTESAYTLKFGTDGTLTDTTGAKARWWVDDQARYCREWLDGKLKGNTGCMNL
                     ELEEGQVALYSEGKKVVEGIVTQK"
     gene            complement(10659..>11238)
                     /locus_tag="PKV97_05315"
     CDS             complement(10659..>11238)
                     /locus_tag="PKV97_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HNB05332.1"
                     /translation="DPVKAVYGVTDFSEAIRNLIMTTLRSIVGEMELDEALSSRDKIK
                     ARLRESIADEAVDWGLTVKSVEIQDIKPSESMQRAMELQAAAERERKAAVTKAEGAKQ
                     AAILEAEARLESAKRDANAQVMLAEASAESIRRVTAGIGDQAGPMMYLLGEKYIAALE
                     KLGDSGSAKIVVMPADLQETLRGLVGRLGARG"
BASE COUNT         1860 a         3838 c         3640 g         1800 t
ORIGIN      
        1 cgctgccacc gatgccgaga atgaagcggc gcgcaccggc gtcgagcgcg gcacggatca
       61 gctcgccggt gccgcgcgag ctggtgacga gcgggttgcg ccggtccggc ggcaccagcg
      121 ccaggccgct ggcggccgcc atctcgatca ccgcggtcgc gccgtcgccg gtgaggccgt
      181 agaaggcctc gacgggctgg ccgagcgggc cgctcaccgt gacgatctcc ttgcggccac
      241 cggtggcggc gaccatggcg tcgaccgtgc cctcgccgcc gtcggccacc ggcaccagca
      301 cgtactccgc gacggggaag acctggcgga agccggccgc gatcgcctgc gcgacctcca
      361 gcgcggacag gctctccttg taggaatcgg gggcgatgac gatcttcttc atgggcaggg
      421 tctccggtcg ctgcgggtcg aatgcccgct gcagaggagc ttagtccctg ggagccgaca
      481 atcaatcgtc ggatatcaca aaataacaac cttcaatcga tagtttatcg tgaaagttaa
      541 ctattttccg cgacatccag ttcgaggccg atgaacagca gcaggcagtc gttcagacgg
      601 gcgagatcca gcccggtgat ctcgccgatg cggcgcaggc ggtagtcgag ggtgttgcgg
      661 tgcacgtgga gcgcgccggc actcggcgcc gccttcatgt cgtgcgcgaa ccacgccgcc
      721 agcgtgcggc gcaactggcc gtcgcggtct tgctgcgcga ggcgccacaa cgggcgacgc
      781 agctcgtcgg cccgccagcc ctcgcccagg cctgcgagca gcaccggcag ggtgagttcg
      841 tcgtagcagt ggatcgccgc ctgcgggcga tggcgtcgcc cggcctgcag cgtggcgcgc
      901 gcggactgcc aggaacgagc caggccatcg tcgccaccga aacggccacc gagggcaaga
      961 catggctgcc cgctgccctc gccgcccagg cgtccgtgca gggcctggag gcgactacgc
     1021 agatcggatt ccgccgccgg caagggcatc agcacggcaa gctcgcgcaa cgtcaccggc
     1081 gcgcaggcaa ggccgggctc ctgctcggcg agcagccggc gcaggcgctg ctgcgcggcg
     1141 aggacggcat cggccgccag cgatgcgtcg gcgatctcga tcagcaaggc gacgcagggt
     1201 cgggcgagat cgacgccaag ccggcttgcc cagtcgtcca gcccggcgcc cggcggggag
     1261 acgccgatca actgcagcac cagctcctcg cgcaggcgct ggtcgcgcgc gagcatctgc
     1321 atccactgcg cctgctcgag catggtctcg gccgcgagac ggaccagctc ggcctgctgg
     1381 cggaccgcgt cgggttcgcc gctcaggccc accacgccca ccacccggcc ttccgcgcgc
     1441 aggggcaggt tgatgcccgg ccgggtgtcg tgcaggcgct gcgcgaccgc cgcgtccacc
     1501 tccaccgccc gcccctgcgc cagcaccagc agcgcaccct cgtggcggct gccgatgcgc
     1561 gcgcgatcgc cgctggcgat gatcaggccg cgctcgtcca tcacattgac gttggtgcgc
     1621 aggatctgca tcgcgcgatc gacgatggac tgggcgagcg aggcatcgag caggaacatg
     1681 gcggcgcacc ggtggcggat tggacataca ccggccacat gctgccgcgc ggcgtgctgc
     1741 ggaacaagcg cggcaccgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1861 cccgcagcgg cgaactgccc gcagaaatcg cccgcctgat cgaacaggcg gccgacgaga
     1921 tcctcgccgg ccgccacgac gcgcagtttc cgctcgtggt gtggcagacg ggcagcggca
     1981 cgcagaccaa catgaacgtc aacgaggtcg tcgccgggcg cgccaacgag ctcgccgggc
     2041 agggccgcgg cggcaaggcg ccggtgcatc cgaacgacca cgtcaatcgc gcgcagagct
     2101 cgaacgactg cttcccgacc gcgatgcacc tcgccgccgc gcgcgcggtg cacgacgagc
     2161 tgctgcccgc gatcaccgag ctgtcgaacg ggctggccat ccaggccgaa cgccatgccg
     2221 gggtgctcaa gaccggacgc acgcacctca tggacgccac gccgatcacc ttcggccagg
     2281 aactgtcggc cttcgtcgcc cagttgaaca ccgccgcgca tgcgatccgt gcggcgctgc
     2341 ccgcggtgtg cgagctcgcc cagggcggca ctgcggtagg caccggcttg aacgcaccac
     2401 cgggtttcgg cgtggaaatc accgccgaac tggcggcgct gaccggcctg cccctggtca
     2461 gtgcgcccga caagttcgcc gcgctcgccg gccacgaacc gctggtggcc ttgtcgggcg
     2521 cgctgaagac gctcgcggtg gcgctgatga agctcgccaa cgacctgcgc ctgctcggct
     2581 cgggcccgcg cgccggcctc gccgagctga agctgccggc caacgagccg ggcagctcga
     2641 tcatgcccgg caaggtcaac ccgacgcagt gcgaggcctt gtcgatgatc gcctgccagg
     2701 tgctcggcaa cgatgccacg atcgccttcg ccgccagcca gggacatctg cagctcaacg
     2761 tgttcaagcc ggtcatcgcc tacaacctgc tgcagtcgat ccgcctgctc gccgacggtt
     2821 gccgcaactt ccagcgccat tgcgtggccg gcctggaggc ggaccacgcg cgcatgtccg
     2881 agcacctcga gcgcagcctg atgctggtca ccgcgctcaa cccgcacatc ggctacgaca
     2941 aggccgcgca gatcgccaag aaggcctggg cggaaggcac cacgctgcgt accgcggcgc
     3001 ttgcgctcgg cttcaccagc gcggaggagt tcgatgcctg ggtgcgcccg caggacatgc
     3061 tcggcaggtg agacctgcat ccggatgtga tcgacgcgac cgccccgcag gcgtgcggac
     3121 ggcgcgccgg cgcgccggaa gggccggaaa tcggcgcttg ccgcgctgcc gcatgaaccg
     3181 ggaagtccga aacaagtcga aacctcgcga ccggatccct gcgtcggatg cgcactcata
     3241 ctgcacaccc ccgacggcgc ggcaatgtct ctgtgctccg cgcaccgacc atcacaagga
     3301 ggccatcatg aagatccgta ccctcgtccc cgccctgctc ctcgccgccg ccgccaccgg
     3361 ctgtgcgacc tgggacaacc tgtcctcgcg cgagaagagc gcggccaccg gcgccgcggt
     3421 cggcggtgtg gccggtgccg tgatcaccga cggcggcatc ctcggcaccg tgggcggtgc
     3481 cgcgatcggt ggcgtgatcg gcgaccaggt cggcaagaag aagtaagccc ccgtcgccgc
     3541 gcgatgccgc cgcgggtgtg tcccgcggcg cggcatcccc ctgattgctt ggtagaatcc
     3601 cgctcgtccc gccgcgcccg accccgggcg ggcggttttt gctttcctgc cgacgagccc
     3661 cgatgaccac cacccccttc ccgcccgaac tgctgcgcga cgtcgagaag cgccgcacct
     3721 tcgccatcat ctcccacccc gacgcgggca agaccacgct caccgaaaag ctgctgctgt
     3781 tcggcggcgc gatccagctc gccggcacgg tgaaggcgag gaagtcggcc cgccacgcca
     3841 cctcggactg gatggaagtc gagaagcagc gcggcatctc ggtgaccagc tcggtcatgc
     3901 agttcgagta ccagggccac accatcaacc tgctcgacac cccgggccac gaggacttct
     3961 cggaagacac ctaccgcgtg ctgaccgcgg tcgacgccgc ggtgatggtg atcgacgccg
     4021 ccaagggcgt ggaagcgcag acgatcaagc tgctcgaggt ctgccgcctg cgcaacacgc
     4081 cgatcatcac cttcgtcaac aagctcgacc gcgaggtgcg cgaacccttc gagctgctcg
     4141 ccgagatcga ggacgtgctc aagatcacct gcgcgccggt gacctggccg ctgggcatgg
     4201 gcaaggcctt ccgcggcgtg tatcacctgc tgcagcacca ggtgctgacc ttcactccgg
     4261 gcgaggagcg ccgcagcgac ggcgagatca tcaagggtct cgacaacccc gagctcgaca
     4321 agcgcttccc gatggaggtc ggccagttgc gcgaggacgt cgagctgatc gacggcgcct
     4381 ccccgccctt cgacctcgac gacttcctcg ccggcaagca gagcccggtg ttcttcggct
     4441 cgggcatcaa caacttcggc gtgcaggaga tcctgcagtc gctgatcgac tgggcgccgc
     4501 cgccgcaggc gcgcgatggc ggcagccgca tggtgcagcc ggccgagccc gcgttcaccg
     4561 gcttcgtgtt caagatccag gccaacatgg acccgaagca ccgcgaccgc atcgccttct
     4621 tccgcatctg ctccggccgc tacagcgccg gcatgaaggt gcgccacgtg cgcgccgggc
     4681 gcgagatgaa gctggccaac gtgctcacct tcatggccaa cgagcgcgtg atcatggagg
     4741 acggcgtcgc cggcgacatc gtcggcatcc acaaccatgg ccagctgcag atcggcgaca
     4801 ccctgaccga gggcgagagc ctcggcttca agggcatccc ctacttctcg cccgagatgt
     4861 tccgcgccgc gcgcctgcgc gatccgctca agtccaagca gctgcagaag ggcctgcagg
     4921 agctcggcga ggaaggcgcg atccaggtct tcgagctcga gggcggccag atgctgctcg
     4981 gcgcggtcgg cgtgctgcag ttcgagatcg tcgcccagcg cctgaaggac gagtacaagg
     5041 tcgatgcgat cttcgaaccg gccgacatcc acaccgcacg ctggctcacc ttccccgatg
     5101 acgtcacccg gcgcaatttc gagcgcgagc aggcgctgcg cctgggcaag gacatcgacg
     5161 gcaacccggt gtatctggcc accagccgct acaacctggc agtgaccgcg gagaagtggc
     5221 ccaaggtgac cttccacgcc acgcgcgagc atggggaagt gctgggctga gcgaggcaac
     5281 cgagcatcgc tgccattcat cgcgcagccc aggccgggct gcgtccccgc tcaggtgctc
     5341 agataacgcg aaaacagggt cgccatcggt ttggctgcag tcgacagcgg caaggcgcgc
     5401 aggcgcagca ggccgaaaca catcggcggc ggcatgggcg tcacgtcgat gcgctgcagg
     5461 ccatagccga cgaacttgtc gtcgaacaga cggcccggac cgatgccgat gcagtccgtg
     5521 cgctgcatga gctcgagcat gagcagggtc gatgaacagt ggatcacgtg cctgggcgtc
     5581 ggcaggccct gctgctccat ccagctcatc aaggcacgtc cgagactgcc ggggctcagg
     5641 ttcatcacga acttggcgtc gcggagttcc gcccaggtcg tggctgcggc cagtgggtgg
     5701 ccttcccgtc cttccagcac ggcattggcg ggtccgagcg gctcgaagat gacttcgtag
     5761 ggcagctcgg cgggatcggc gaccgcgatc gcaaagtcga ggcgcccctc gatcaggtcg
     5821 ggcagaacct ggctcagcaa gccttcggtc aggtgcacct cggcgtccgg acacaatttt
     5881 tcgtactcgc gcaacacgcg gggcagggct accgcccccg ccatgggcgt gacggcgagg
     5941 accacgcgtg cgcctgcacc gccccgcagc aggcggattt ccctcgccgc cttgtcgaga
     6001 atcgacagcg ccagccgtgc atggctgagc agcatgctgc ccgccggggt gaactcgatg
     6061 cccttgtagc tgcgggtcat gagttcctca cccacatctt cttccagttc gcgcagcgac
     6121 ttggtcaggg cgctctggct caggtgcatc gaacgggccg ccgcacgaat cgaactgcat
     6181 tccgcgacct gcaccagcgc tcgaagttga tgctctttca tcggtttcgt tcggaatatc
     6241 tcggagtaag tccagacaat cgactaagtc ttattgtcgg gatcataagc cgataaaccg
     6301 tccggggtga ttccaaacgg tcttcaccac agtcaatgaa atgccttttg ccgctcgagc
     6361 gaatgaataa catggccgcg tgaattccat ccagagccct tcattcagga gatcgacatg
     6421 acagccgtcg tgggtacacc aagcactgcc taccaaggca atgacaagct gctgtatggc
     6481 atcatcttag gcgtcatcac attctggctt tttgcacaga cggtcctcaa cattgcgccg
     6541 gacatgcaaa aggacctcgg tctcgatgcc agcatgatga acaatgcggt ggccatcacc
     6601 gcactctttt ccggcatctt catcgtcgtc atcggcgggc ttgcagaccg tatcggccgc
     6661 gtgaagaccg ttcagatcgg cttcctgctc agcatcgtcg gttccctgct gatcgccctc
     6721 gcccccaccg gcagcctggc gaccaccttc ctgctggtag gtcgtgccct gcagggggtt
     6781 tcggcagcct gcatcatgcc agccagtctc gcgctggtga agacgtactg ggacggtccg
     6841 gcccgtcagc gcgcggtgag cctgtggtcc atcggctcct gggggggttc gggtgtctgt
     6901 tcgctgttcg gcggcctggt cgcggagaac ctcggctggc gttacatctt cttcgcctcg
     6961 atcgtcgtcg ccgtgatcgg catgctgatg gtcaagggca caccggaaag caaggctgaa
     7021 accaaaggcg cctacaagtt cgacctgccg ggcgtgctga ccttcatgat cagcatggtg
     7081 gccctgcaga tcgtcgtcac tcaaggcaac aagatgggct ggaccagccc gctgatcatg
     7141 ggtctgatcg ccgtcgtggt gatcttcggc accctgttct tccgcatcga aaacaccagc
     7201 ggccagccct tcgtcgattt caagctgttc cagaacccga cctataccgg cgccaccctg
     7261 tccaacttcc tgctcaacgg tgtggccggc accctgctgg tttcgctgca actggttcaa
     7321 ctgggcggca acatgacggc gcagcaagcc ggtggcctga ccctgggtta tgcgatcgcg
     7381 atcatcgcct tcatccgtgt cggtgaaaag ctgctgcaac gcttcggcgc ccgcaagccg
     7441 atgatctggg gttgcctgat cgtgggcgtg tcgatcgtga tgctgtcccc ggccaacctg
     7501 ctgctcgagg actacaacac cctcgccatc atcggctaca ccctgttcgg ggtcggtctg
     7561 gccttctacg ccacgccttc gaccgacgcc gcgctgtccg cgctaccggc cgcccaggcc
     7621 ggttcgggcg cgggcatcta caagatggct tcctcgctgg gcgccgcctt cggtgtggcg
     7681 atctccgctg cgatcttcac cgcgctcagt gccaaccccg agagcatcgc ctggctcgaa
     7741 ggcgtgatca ccttcgccgg acgccaggac aacctggccg tgcgcgaggc agcgatcatc
     7801 gcgctgatgt tcaacgtctt catggtggtg gtggcgatca tgtcgatcat gctcaccgtg
     7861 ccgaagttcg tcggcaagaa gaactgaccc gcttccaggg caaaccctct ccgcgccgtg
     7921 cgcagtggat tccagcatgg cgcgtgcgca cagtcagccc gggccgatcg catcggtccg
     7981 cacggcccgg gcgtttcatc aaccggtttc agatttcgat cgggagtcat catgagcacg
     8041 cttttgcaag agttgtcggc gaatcaggaa gcgatgaaga cctggcgtca ccacatgcac
     8101 cgccaccccg aggtcgcgtt cgacgagcac aagacggcca gctacatcgc cggcctgctg
     8161 cgggagtggg gatacgaggt caccgaaggc gtcggcaaga cgggcgtggt ggccaggatg
     8221 cgctgcggcg acagcaagaa gtcgatcggc ctgcgtgccg ataccgacgc cctcgccgtg
     8281 caggaggcga atgacagcga ccacaagagc accatcgaag gcaagtcgca tacctgcggt
     8341 catgacggtc atagcgccat gctgctgggg gctgcgcagc acctggcgcg cactcgtgcg
     8401 ttcaacggca cggtcaacct gatcttccag cccgccgaag aaatcatggg cggcgcggtc
     8461 gccatgatcc aggacggtct gttcgagcgc ttcccgatgg atgcggtctt cggcatgcac
     8521 aacatgcccg cgctggagca gggcaagatg tacttcacct ccggcccgct gttcgccgcg
     8581 gcggacaact gggaaatcgt gctgaccggt gtcggcagcc acggctcgat gcccgagaaa
     8641 agcatcgacc cggtggtggc gggagcttcc ctggtcatgg cgctgcagac catcgtctcg
     8701 cgcaacgtcg cggccaagga ttcggccgtg gtgagcgtcg gcgccttcct ggcgggcgat
     8761 gccggcaacg tcattccgca gaccgcgacc ctgcgcctga gcacccgcac cagcaaaccg
     8821 gaaacgcgtc agctggtgct cgccaagatc aagtccatca ccgcggcaca ggccgaggcc
     8881 ttcggggtcc gctacgaaat ccgcgaaggt tcgccgggtg ccgtgctggt caatgatccc
     8941 gcgctgaccg aggagtgtgc cgacgtcgcc cgcaagctgc tcggcgagga tcaggtggtg
     9001 atgcccggac cgacctacat gggcagcgag gatttcgcct ttttcgccca gctcaagccg
     9061 agcacctact gcatcatcgg caacggcgac accgccatgg ttcaccatcc caagtacgac
     9121 ttcgacgacg acaacctgcc cgtgggcgcc gcctactggg tggcgctgac ccagcaccac
     9181 ctgaagtaag cgcggcaacc aggcgctgga aacgtctgca gcctcgacga gccggtcgcc
     9241 gaggccttgc aggcccgtgg cgcgcggaag tttccgcaaa tcgcggcgat tcgtccgccg
     9301 cggcctccgc aagcaatggg ctgtccacct ggccgcggcc gaagtgcccc ccggaccatc
     9361 cacaccaccg ctcgccatcg cccgtcgcga tggtctggat gcgctgctgc gagcatctcg
     9421 agcacaccct cgccgccatc ccggcgccgg tgtaggaagt cccgtcaaac gggtgggcaa
     9481 ccgcgatcgc cggccgcctc cacccagtag gtctgcttgt gtccgtgcgc ctcgaaggcg
     9541 cgcacggccg cctccgcctc gtcccggcag gcgaacaccg ccaccaggaa gcggttgccg
     9601 ttgtcgtcga gccgccacag tgcccagccc gcgatcggct ccgcggcgcg cgcgttttcc
     9661 gtcaccagtc aggcgagaag ctggtgcgcg aaggcctcga cctgcgtcca gtcggtgaaa
     9721 tccgtgaccg agcgcgggtc ggtggggccg ccggtgatcc acatgatcag gcggatcatg
     9781 ttgcggtcga agaagccgta gcgcgggtat tcgatcttgc cgccgaagac ctccacctgc
     9841 tgcggcttcc agcgcagctg gccgaggaac ttgcgcacgt aggggttggt gtcggcctgg
     9901 cgcttgtgcg gcttgcggcc gaccgcgttg accgagaaga aggcgttcgg gcgcacctcg
     9961 agcgccgcct ggtggcggtc gatgaaggcc ttcaccgcgg gacgatggtg accgtagcgg
    10021 atgctggcgc cgatcacgat gcggtcgcag gcctcgagct gttcagcgct ggccgactcc
    10081 acctcggcca gctcgacctc gtgaccggtc gcacgcgcga tcgcggcgat gcgctcgcag
    10141 atctcgagcg tatggccgtc gatggtggaa tagacgatca gcagtcgcat gcccgctccg
    10201 gtcgcgcagg tcatttttgg gtgacgatgc cctcgaccac cttcttgcct tcggagtaga
    10261 gcgctacctg gccttcctcg agctcgaggt tcatgcagcc ggtgttgccc ttgagcttgc
    10321 cgtcgagcca ttcgcggcaa tagcgcgcct ggtcgtccac ccaccagcgc gccttggcgc
    10381 cggtggtgtc ggtgagcgtg ccgtcggtgc cgaacttgag cgtgtaggcc gattcggtgc
    10441 gcagatacac gccgatcaag gtggcgccgg tgagcagctt ctcgacctcg gccttgtcct
    10501 tcatgaagtt ctgcgccagc gcgccagtgg agaccgtgac cagtgcgacg gcggcaatca
    10561 ggctacggac gggtttcgag tatttgcgca tcgttgtctt cctttgttgc gggtacggat
    10621 ccggggaaag cgatcggaga cgccgggctg gagcggattc agccgcgcgc gcccagccgg
    10681 ccgacgaggc cgcgcaaggt ctcctggagg tcggcgggca tcaccacgat cttggcgctg
    10741 ccggagtcgc ccagcttctc gagcgcggcg atgtacttct cgccgagcag gtacatcatc
    10801 gggcccgcct ggtcgccgat tccggcggtg acgcggcgga tcgactcggc cgaggcctcg
    10861 gcgagcatga cctgggcgtt ggcgtcgcgc ttggcggatt cgagccgcgc ctcggcctcg
    10921 aggatggcgg cctgcttggc gccctcggcc ttggtcaccg cggccttgcg ctcgcgctcg
    10981 gcggcggcct gcagctccat cgcccgctgc atggattcgg aaggcttgat gtcctggatc
    11041 tcgaccgact tcaccgtgag gccccagtcg accgcctcgt cggcgatgct ctcgcgcagg
    11101 cgcgccttga tcttgtcgcg cgaggacagc gcctcgtcga gctccatctc gccgacgatc
    11161 gagcgcagcg tggtcatgat caggttgcgg atcgcctcgg agaagtcggt cacgccatac
    11221 accgccttga ccgggtcg
//
LOCUS       DAOQNN010000058        11149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2712_length_11149_cov_10.431044, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000058 DAOQNN010000000
VERSION     DAOQNN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2712_length_11149_cov_10.431044"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2210
                     /gene="secA"
                     /locus_tag="PKV97_05320"
     CDS             <1..2210
                     /gene="secA"
                     /locus_tag="PKV97_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNB05333.1"
                     /translation="TYGTNNEFGFDYLRDNMVYAVGERVQRALAFAIVDEVDSILIDE
                     ARTPLIISGQAEDHTDLYLKMNQVAPMLKRQEGGLDDKDEVIEAGDYTVDLKAHQVLL
                     TEAGHETAEQILVRMGLLAEGGGLYDPGNILLVHHLYAALRAHALYHRDQQYVVQNGE
                     VVIVDEFTGRLMPGRRWSDGLHQAVEAKEGVRIQAENQTLASITFQNYFRMYGKLAGM
                     TGTADTEAFEFQQIYGLETVVIPTNRPTRRRDENDKVYRTASEKWNAVIEDIRACVER
                     GQPVLVGTTSIETNEFLSGVLKKAKIEHQVLNAKQHDSEAQIVAQAGRPGMVTIATNM
                     AGRGTDIVLGGNIEKPVAAVRDDAALDPAQREARIAALREAWQKVHDEVIAAGGLHII
                     GTERHESRRIDNQLRGRSGRQGDPGSSRFFLSLEDPLMKIFAGERLNAIMVRMKMPEG
                     EAIEHAMVTRSLESAQRKVEQRNFDIRKQLLEYDDVANDQRKVIYQQRNELLESEDIS
                     DTVRAMRQGVLHDIFRRYVPVDSVEEQWELPALEQALLAECQLKLAVAAWVTAEPSLD
                     DEAILERVVQAGEEAYAAKTAMVDPAAWHQFERNVMLQSLDTHWREHLAALDHLRQGI
                     HLRGYAQKNPKQEYKREAFELFESLLDAVRTDVTKILMTVQIRTEAQLEEAETPQQVE
                     NVQYQHADYDEALGGGAEQAGAQPATAGPKVGRNDPCPCGSGKKYKHCHGKLN"
     gene            2233..3471
                     /gene="argJ"
                     /locus_tag="PKV97_05325"
     CDS             2233..3471
                     /gene="argJ"
                     /locus_tag="PKV97_05325"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNB05334.1"
                     /translation="MAVNLATPNPAELLPVKGVRLGVTEAGIRKANRRDLTVIELSEG
                     SRVAGVFTQNRFCAAPVQVCRQHLTGGAIRALLINTGVANAGTGEPGLANANACCAAL
                     ARELGIEAGQVLPFSTGVILEPLPVDRLVAGLPGAVADLRADGWFDAAHAIMTTDTLA
                     KAVSRQVRIGERTVTLTGISKGAGMIRPNMATMLGFLACDAAIAQDLLDDLVREAADL
                     SFNSITVDGDTSTNDSFILIATGAAGNAEIADADSEDYRALRAAVIEVAVGLAQAIVR
                     DGEGATKFMTIAVEGGRDREECRKVGYAVAHSPLVKTAFFASDPNLGRILAAIGYAGI
                     DDLDVAKLRVWLGNPEESVLVAEHGGRAASYLEEAGSRIMKHAELTVRIALARGDAAA
                     TVWTCDFSYDYVKINADYRS"
     gene            3680..5734
                     /locus_tag="PKV97_05330"
     CDS             3680..5734
                     /locus_tag="PKV97_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB05335.1"
                     /translation="MQDPYTSRDPRTRGLASRAGPLVLLGLLALDARAEWGDPDPFFS
                     EIPMVLTASRLAQSPLDAPAPVTVIDREMIVASGFNEIHDLLRLAPGFLVADHPDGPP
                     TVATHGLGDAHARRIKVMVDGRTVNSPLWGNVIWDNLPLRVDDVERIEVVRGPNGAAY
                     GVNAFQGVVNIITRAPSTESGRAFVVRTGKNGFRDYGVRLNGAGGSAFDWRLSASRRE
                     LTTFEPHFRERLGDWHSSESLTRNTAHFSAVLQLSAAEELGLQVGVSEGDSKRGAQAY
                     LDYPPHIEEDRALHLQFGWRRQIDAESVLSVQYFHQGERAGGSWPVIQAQGTTTASFP
                     TSRASEARRHDLELQYDTRLGRRLTGMLGIGLRSEHARSLHLFGTTRWLDSTHGQVFG
                     SLTWNPLDPLKIDLGGTLEHHDYSGRLFSPRLAFNYALDEDSALRLSTGTAYRAPSLM
                     ESRAFQTDREGDTIRRIGFRVFDPLQSERMRFVDLGYVAQLRSFGLGFDARVFREYYR
                     RFIDDKSCVFGECPRPEPADYVPIDPGKKSFYFLNVDDFRMTGAEFTLDWRKPGWGRA
                     VLSQAFIRIDHGEPYSDRDIPNSAPHATTSLLLIKDFPGRWQASLGYYHNGLYYWMNG
                     GDIVPRTDRFDLRVARRFGPPGSDNEVSLTALSLKGRYADFHEDKFRHEPDLYLGVRI
                     GW"
     gene            5803..6693
                     /locus_tag="PKV97_05335"
     CDS             5803..6693
                     /locus_tag="PKV97_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNB05336.1"
                     /translation="MLALLPVLGLSVAHAMEVAVLVSEPGGRGQRFVEALDAALAPGV
                     HRLAGAGLAGGEADTALLGRADLVLAVGHAAAERALRDSSRPVLAVLVGLRDLDRLKR
                     AHPARDLGAVVLDQPLERHLRLVRAVLPDAKRVGVLLGPDSVVQLPGLQHAADALRLQ
                     ADVHEVREVADLVPALDHLLFAGDALLPLPDGLVSGPAAARTILLTSYRHRRPVFAHS
                     AAYVTAGALAAVFSTPEHVAVDVSDLLAAAKEPAALVRKIHSPRRFDVAVNRAVARAL
                     GLVVADEQVLRERIADGGAQ"
     gene            6690..8024
                     /locus_tag="PKV97_05340"
     CDS             6690..8024
                     /locus_tag="PKV97_05340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB05337.1"
                     /translation="MIAALSRYSLRRQMLLVLGLPSVVFVLCIAALFAYQSSRALDQA
                     LLERGRAMVRFLAPAAEYVLVSGNREALGALMARVQVQPDVVGVGFYDPGGDALATLG
                     EGLSPRLAAPSRAEGDEIRVDLADGAIGFEAPVVAVPATVDDYAEDSSSKTAPVVGQV
                     RVVLGTARLDAEKRGVFLTVSLLVLGVLATAMAIAVRLAGSVGEPVAALVDAVGRMAD
                     GDLAARVPARARNGELKALEAGFNAMAEAIAENQRTLQARIDEATVQLAWQARHDPLT
                     GLANRRAFEERIEQAVRAHRRSGDQITLFYIDLDHFKVVNDSCGHAAGDRLLSELGAL
                     LKGRLRSEDEVFRIGGDEFAAILRGCGREDARRIAEGLCAAIAAHAFESDGRAFRVGA
                     SIGLARMDPDLQEADALMIAADQACYAAKHGGRGHVVEYPLPFVAPGSARPG"
     gene            complement(8063..8446)
                     /gene="apaG"
                     /locus_tag="PKV97_05345"
     CDS             complement(8063..8446)
                     /gene="apaG"
                     /locus_tag="PKV97_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNB05338.1"
                     /translation="MSETTPYRVQVTAVAEYVADQSRPQDEHFVFAYHITVLNTGEVR
                     AQLLARHWVITDGNGKMQEVHGQGVVGEQPVLGPGQAFRYTSGCVLATPVGTMQGSYR
                     MRADDGHEFEAEIPAFMLAVPKALH"
     gene            8515..9891
                     /gene="purB"
                     /locus_tag="PKV97_05350"
     CDS             8515..9891
                     /gene="purB"
                     /locus_tag="PKV97_05350"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNB05339.1"
                     /translation="MSNAAPSPLTALSPLDGRYHGKVAGLREHFSEHGLIRNRVRVEV
                     EWLKALAAEPALAEIAPFSPATVVELDAVVAQFSTDDGEAVKAIEATTNHDVKAMEYW
                     LKKRLGHNAEVMKVSEFIHFACTSEDINNTSHALMLAEGRDRVLLPALDAVIARFREL
                     AHALADLPMLSRTHGQPASPTTLGKEMANIAARLMRARAAIAGVAMTAKFNGAVGNYN
                     AHLSAWPQFDWEGFNRRFIESLGLTFNEYTIQIEPHDAMAELFDAIARANTMLIDACR
                     DIWMYISLGYFKQKLKEGEVGSSTMPHKVNPIDFENAEGNFGLANAVLKHFSEKLPIS
                     RMQRDLTDSTVLRNMGVGFGHTVLALDSCLRGLGKLEAEPARLAADLDDCWEVLAEPV
                     QTVMRRYGIANPYEQLKAMTRGKGITREALQEFIRTLAIPAEARDHLLAMTPASYVGK
                     AAELARRI"
     gene            9976..10293
                     /locus_tag="PKV97_05355"
     CDS             9976..10293
                     /locus_tag="PKV97_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HNB05340.1"
                     /translation="MAFADNLKTLPGVSHLAALNLIDAADAVVATIENKPGQAGSLAV
                     YNHLAQLYGAISPEAAKKGLELYAEHTEDARANPGKHPNIDRLIGLIERGETLRVKQV
                     FAV"
     gene            complement(10601..>11149)
                     /gene="secF"
                     /locus_tag="PKV97_05360"
     CDS             complement(10601..>11149)
                     /gene="secF"
                     /locus_tag="PKV97_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNB05341.1"
                     /translation="QVGKELAADGSMALLLVIVGIMIYLALRFEWRFAVSAIIANLHD
                     VIIILGFFAFFQWEFSLPVLAAVLAVLGYSVNESVVVFDRVRETFKKKRNMTTPQVLD
                     HAITSTISRTVITHGSTQVMVLSMLVFGGETLYYFSLALTIGICFGIYSSVLVASPLV
                     MWLGVSREQFIKPKKEKEEAVV"
BASE COUNT         1773 a         3916 c         3769 g         1691 t
ORIGIN      
        1 tcacctacgg caccaacaac gagttcggct tcgactacct gcgcgacaac atggtgtatg
       61 cggtcggcga gcgcgtgcag cgcgcgctcg ccttcgcgat cgtggacgag gtcgactcca
      121 tcctcatcga cgaggcgcgc acgccgctga tcatctccgg ccaggccgag gaccacaccg
      181 acctctacct gaagatgaac caggtcgcgc cgatgctcaa gcgccaggaa ggcggcctcg
      241 acgacaagga cgaggtcatc gaggcgggcg actacacggt cgacctcaag gcgcaccagg
      301 tgctgctcac cgaggcgggc cacgagaccg ccgagcagat cctcgtgcgc atgggcctgc
      361 tcgccgaggg cggcggcctc tacgaccccg gcaacatcct gctggtgcat cacctctacg
      421 cggcgctgcg tgcccacgcc ctctaccacc gcgatcagca gtacgtggtg cagaacggcg
      481 aggtcgtcat cgtcgacgaa ttcaccggcc gcctgatgcc cggccggcgc tggtccgacg
      541 gcctgcacca ggcggtcgag gccaaggaag gcgtgcgcat ccaggccgag aaccagaccc
      601 tggcctcgat caccttccag aactacttcc gcatgtacgg caagcttgcg ggcatgaccg
      661 gcacggccga caccgaggcc ttcgagttcc agcagatcta tgggctcgag accgtggtga
      721 tcccgaccaa tcgcccgacc cggcgcaggg acgagaacga caaggtctac cgcaccgcga
      781 gcgagaagtg gaacgcggtc atcgaggaca tccgtgcctg cgtcgagcgc ggccagccgg
      841 tgctggtcgg cacgacctcg atcgagacca acgaattcct ttccggcgtg ctcaagaagg
      901 cgaagatcga gcaccaggtg ctcaacgcca agcagcacga cagcgaggcg cagatcgtcg
      961 cgcaggccgg ccggcccggc atggtcacga tcgcgaccaa catggccggc cgcggcaccg
     1021 acatcgtcct cggcggcaac atcgagaagc cggtcgccgc ggtgcgcgac gacgccgcgc
     1081 tcgaccccgc gcagcgcgag gccaggatcg ccgccctgcg cgaggcctgg cagaaggtgc
     1141 atgacgaggt gatcgccgcc ggcggcctgc acatcatcgg caccgagcgc cacgagtcgc
     1201 gccgcatcga caaccagctg cgcggccgct ccgggcgcca gggcgacccg ggctcgagcc
     1261 gcttcttcct ctcgctcgaa gatccgctga tgaagatctt cgccggcgag cgcctgaacg
     1321 cgatcatggt ccgcatgaag atgcccgagg gtgaggcgat cgagcacgcg atggtgacgc
     1381 gctcgctgga gtcggcgcag cgcaaggtcg agcagcgcaa cttcgacatc cgcaagcagc
     1441 tgctcgagta cgacgacgtc gccaacgacc agcgcaaggt gatctaccag cagcgcaacg
     1501 agctgctcga gagcgaggac atctccgaca ccgtgcgcgc gatgcgccag ggcgtgctgc
     1561 acgacatctt ccgccgctac gtgccggtcg acagtgtcga ggagcaatgg gagctgcccg
     1621 cgctcgagca ggcgctgctc gccgagtgcc agctcaagct ggcggtggcc gcgtgggtga
     1681 cggccgagcc cagcctggac gacgaggcca tcctcgagcg cgtggtgcag gccggcgagg
     1741 aggcctacgc cgccaagacc gcgatggtcg acccggcggc ctggcaccag ttcgagcgca
     1801 acgtgatgct gcagagcctc gacacccact ggcgcgagca cctcgcggca ctcgaccacc
     1861 tgcgccaggg catccacctg cgcggctatg cgcagaagaa ccccaagcag gaatacaagc
     1921 gcgaggcctt cgagctcttc gagagcctgc tcgacgccgt gcgcactgac gtcaccaaga
     1981 tcctgatgac ggtacagatc cgcaccgagg cgcagctcga ggaagccgag acgccgcagc
     2041 aggtcgagaa cgtgcagtac cagcacgccg actacgacga ggcgctcggc ggtggcgccg
     2101 agcaggcggg cgcgcagccg gccactgccg ggcccaaggt cggccgcaac gacccctgcc
     2161 cctgcggctc gggcaagaaa tacaagcact gccacggcaa actgaactga tccttccgtc
     2221 ccggagaacc gcatggccgt caatctcgcc accccgaacc ctgccgaact gctgcccgtg
     2281 aagggcgtgc gcctcggcgt gaccgaagcc ggcatccgca aggccaaccg gcgcgacctt
     2341 accgtgatcg agctgtccga gggcagccgc gtcgctggcg tgttcacgca gaaccgcttc
     2401 tgcgcggcgc cggtgcaggt gtgccgccag catctgacgg gcggggcgat ccgcgcgctg
     2461 ctcatcaaca ccggtgtcgc caacgccggc accggcgagc cgggcctggc caacgccaat
     2521 gcctgctgcg ccgcgctggc gcgcgaactg ggcatcgagg cgggtcaggt gctgccgttc
     2581 tcgaccggcg tgatcctgga gccgctgccg gtcgaccgcc tggtcgccgg gctgcccgga
     2641 gcggtcgcag acctgcgtgc cgacggctgg ttcgatgccg cgcacgcgat catgaccacc
     2701 gacaccctcg ccaaggcggt gtcgcgtcag gtccgcatcg gcgagcgcac ggtcaccctc
     2761 accggcatca gcaagggcgc aggcatgatc aggcccaaca tggccaccat gctcggcttc
     2821 ctcgcctgcg acgcggcgat cgcgcaggac ctgctcgatg acttggtgcg cgaggcggcc
     2881 gacctgtcct tcaacagcat caccgtcgat ggcgacacct cgaccaacga ttccttcatc
     2941 ctcatcgcca ctggcgccgc cggcaatgcc gagatcgccg acgccgacag cgaggactac
     3001 cgcgccctgc gcgcggcggt gatcgaggtc gcggtcgggc tcgcgcaggc gatcgtgcgc
     3061 gatggcgagg gcgcgaccaa gttcatgacg atcgcggtcg agggcggccg cgaccgcgag
     3121 gaatgccgca aggtgggcta tgcggtggcg cactcgcccc tggtcaagac cgcctttttc
     3181 gcctccgacc ccaacctcgg ccgcatcctc gcggcgatcg gctatgccgg catcgacgac
     3241 ctcgacgtcg ccaagctgcg cgtgtggctg ggcaaccccg aggagtcggt gctggttgcc
     3301 gagcacggcg gtcgtgcggc gagctacctc gaggaggcgg gctcgcgcat catgaagcac
     3361 gccgagctca cggtgcgcat cgcgctcgcc cgcggcgacg ccgccgccac ggtgtggacc
     3421 tgcgacttct cctacgacta cgtgaagatc aacgccgact accgcagctg agcgccttcc
     3481 ggcgtgcgcc acggccccgg cagccactcg gcgccggggc cgtgtcatgt catgcagggc
     3541 gggcggcgac cgtgtgctgg catgcgtgac gtggttcacc caaggcggcg cacgtatctt
     3601 gtcggcgccc ccggaatggg cagaatgcag gctggatggt gcgctcagtt cgccatggag
     3661 ccgtaggaat cgctttgtca tgcaggatcc gtacaccagt cgcgacccgc gcacgcgggg
     3721 tctggcctcg cgcgcggggc ccctcgtcct gcttggcctg ctcgcgctcg acgcgcgtgc
     3781 ggaatggggc gacccggacc cgttcttcag cgagattccg atggtgctga ccgcatcgcg
     3841 cctggcgcag tcgccgctcg acgcgcctgc gccggtgacc gtgatcgatc gcgagatgat
     3901 cgtcgcctcg ggtttcaacg agatccacga ccttctgcgc ctggcgccgg gcttcctcgt
     3961 ggccgaccat cccgatggtc cccccacggt ggcgacccat ggcctgggcg acgcccatgc
     4021 gcgccgcatc aaggtgatgg tggacgggcg cacggtcaac agcccgctgt ggggcaacgt
     4081 gatctgggac aacctgccct tgcgggtgga cgatgtcgag cgcatcgagg tggtgcgcgg
     4141 ccccaacggt gcggcctatg gcgtcaatgc cttccagggg gtggtcaaca tcatcacgag
     4201 ggctccctcc accgagagcg ggcgggcgtt cgtcgtgcgc actggaaaga acggtttccg
     4261 tgattacggc gtgcgcctga acggggctgg cggaagcgcc ttcgactggc gcctctccgc
     4321 ctcgcggcgc gagctgacga cgttcgaacc gcactttcgg gaacggctcg gggactggca
     4381 ctcttccgag tcgctcaccc gcaacaccgc ccacttcagc gcagtgctgc agttgtccgc
     4441 agccgaagag ctcggcctcc aggttggcgt cagcgagggc gacagcaagc gcggcgccca
     4501 ggcctacctc gactatccgc cccacatcga ggaggaccgc gcgcttcacc tgcaattcgg
     4561 ctggcggcgc caaatcgatg cggaatccgt gctgagcgtg cagtatttcc accagggcga
     4621 gcgtgccggc gggagctggc cggtgatcca ggcgcaaggc acgacgaccg cgagcttccc
     4681 gaccagccgg gccagcgagg cgaggcggca cgatctcgag ctgcaatacg acacccggct
     4741 cggccggcgt ctcaccggca tgctcggtat cgggctgcgc agcgagcatg cgcgctcgct
     4801 gcacctgttc ggcaccacac ggtggctgga ttcgacccat gggcaggtct tcggcagcct
     4861 cacgtggaac ccgctcgatc cgctcaagat cgatctcggc ggcacgctcg agcaccacga
     4921 ctacagcggc aggctgttct cgccccgcct tgcgttcaac tacgccctcg acgaggactc
     4981 ggccctgcgc ctgtccaccg ggacggcgta ccgcgccccc agcctgatgg agtcgcgagc
     5041 cttccagacc gaccgcgagg gcgacacgat ccggcgtatc ggcttccgtg tcttcgaccc
     5101 gctgcagagc gagcgcatgc gcttcgtcga tctgggctat gtcgcgcagc tgcgttcgtt
     5161 cggactcggc ttcgatgcgc gtgtctttcg cgagtactac cggcgtttca tcgacgacaa
     5221 gagctgcgtg ttcggagaat gcccgcgccc cgagccggcc gactatgttc cgatcgaccc
     5281 cggcaagaag agcttctatt tcctcaacgt cgacgacttc cgcatgaccg gtgcggaatt
     5341 cacccttgac tggcgcaagc cgggctgggg cagggcggta ctgtcccagg ccttcatccg
     5401 tatcgaccat ggcgagccct acagcgaccg cgacatcccg aacagcgcac cgcatgcgac
     5461 cacctcgctg ctgctgatca aggacttccc cggacgctgg caggcgagcc tgggctacta
     5521 ccacaacggc ctgtactact ggatgaacgg cggtgacatc gtgccgcgca ccgatcgctt
     5581 cgacctgcgc gtcgcgcgcc gcttcggtcc gcccggttcg gacaacgagg tctcgctcac
     5641 cgcgctgagc ctgaaggggc gctacgcgga cttccacgag gacaagttcc gccacgagcc
     5701 ggacctctat ctcggcgtgc gtatcggatg gtgacggcgc gcatgcgagc ctgcctgtcg
     5761 tgtctcttcc ccggcggcct gcgcgtcgca gcggcgctcg ccatgctcgc gctgctgcct
     5821 gtgctcggcc tgtccgttgc gcacgcgatg gaggtggcgg tgctggtttc cgagccgggg
     5881 gggcgcggcc agcgcttcgt cgaggcgctc gatgccgcgc tcgcgcccgg ggtgcatcgg
     5941 ctggcaggcg cggggctcgc cggcggggag gccgatacgg cgctgctcgg ccgcgccgat
     6001 ctcgtgctcg cggtcggcca cgcggcggcg gagcgcgccc tgcgggattc ctcgcggccg
     6061 gtgctggccg tgctcgtcgg cctgcgtgac ctggatcgcc tcaagcgggc gcatcccgcg
     6121 cgggatctcg gcgcggtggt gctcgaccag ccgctcgaac gtcatctccg tctcgtgcgc
     6181 gcggtcctgc ccgacgcgaa gcgcgtcggc gtgctgctcg gaccggacag cgtcgtgcaa
     6241 ctgcccggcc tccagcatgc agcggacgcg ctgcgcttgc aggccgacgt ccatgaggtg
     6301 cgcgaggtgg cggacctcgt gccggcgctc gatcacctgc tctttgccgg cgacgccctg
     6361 ctgcccctgc ccgacggcct tgtctcgggg ccggccgcgg cgcgcacgat cctgctcacc
     6421 agttaccgcc accgccggcc ggtgttcgcc cactcggccg cctatgtcac cgcgggtgcg
     6481 ctcgccgcgg tgttcagcac gccagagcac gtcgcggtgg atgtctcgga cctgctggcg
     6541 gcagccaagg agcctgcggc cctggtgcgc aagatccact cgccgcgccg cttcgatgtc
     6601 gcggtcaatc gcgcggtggc gcgggcgctg gggctcgtgg tcgcggacga gcaggtgctg
     6661 cgcgagcgga ttgccgacgg aggggcgcaa tgatcgccgc gctttcccgt tactcgctgc
     6721 ggcgacagat gctgttggtg ctcggcctgc cgagcgtcgt gttcgtgctc tgcatcgccg
     6781 cgctcttcgc ctaccagagc agccgcgcgc tcgaccaggc cctgctcgaa cgcggccgtg
     6841 ccatggtgcg cttcctcgca ccggcggctg aatacgtgct cgtgtcgggc aaccgcgaag
     6901 cgctcggcgc gctgatggcg cgggtgcagg tacagcccga cgtggtcggc gtcgggttct
     6961 acgatccggg cggcgacgcg ctggcgacgc tgggagaggg gttgtcgccc cggctggccg
     7021 cgccgtcgcg cgccgagggg gacgagatcc gtgtcgacct cgcggacggt gcgatcggct
     7081 tcgaggcgcc ggtggtggcg gtgcccgcga cggtggacga ctatgcggag gattcgtcgt
     7141 cgaagacggc gccggtcgtc ggccaggtcc gcgtggtgct cggcaccgcg cgtctggatg
     7201 ccgagaagcg cggggtcttc ctgaccgtga gcctgttggt gctgggcgtg ctcgcgacgg
     7261 cgatggcaat cgcggtgcgt ctggccggat cggtcggcga gccggtcgct gccctggtgg
     7321 atgcggtcgg gcgcatggcc gacggcgacc tcgcggcgcg cgtaccggcg cgtgcgcgca
     7381 acggcgagtt gaaggcgctc gaagcgggat tcaacgccat ggccgaggcg atcgccgaga
     7441 accagcgcac gctgcaggcc cgcatcgacg aggccaccgt gcaactggcc tggcaggccc
     7501 gccacgaccc gctgacgggg ctcgccaacc ggcgcgcctt cgaggagcgc atcgagcagg
     7561 cggtgcgtgc gcaccggcgc tcgggcgacc agatcaccct cttctatatc gacctcgatc
     7621 acttcaaggt ggtcaatgac agctgcggcc atgctgcggg agaccggctg ctgagcgagc
     7681 tcggcgccct gctcaagggg cgcctgcgct ccgaggacga agtgttccgc atcggtggcg
     7741 acgagttcgc cgcgatcttg cgcggttgtg gccgcgagga cgcgcgccgc atcgccgagg
     7801 ggctgtgcgc ggcgatcgcc gcgcatgcgt tcgagtccga cggcagggcc ttccgcgtcg
     7861 gcgccagcat cgggctcgcg cgcatggacc cggacctgca ggaggccgac gcgctgatga
     7921 tcgccgccga ccaggcgtgc tacgcagcca agcacggcgg ccgtggtcac gtggtggagt
     7981 atccgctgcc attcgtcgcc ccggggagcg cgcgcccggg ctagcagctg tgcacgatcg
     8041 tccgccgtga ccggcagcgc gctcagtgca gcgctttcgg caccgccagc atgaaggccg
     8101 gaatctcggc ctcgaactcg tggccgtcgt ccgcgcgcat gcggtagctg ccctgcatcg
     8161 tccccaccgg cgtggcgagc acgcagccgc tggtgtagcg gaaggcctgg ccggggccga
     8221 gcaccggctg ctcgccgacc acgccctggc cgtggacttc ctgcatcttg ccgttgccgt
     8281 cggtgatcac ccagtggcgc gcgagcagct gggcgcgcac ctcgccggtg ttgaggacgg
     8341 tgatgtggta ggcgaacacg aagtgctcgt cctgcgggcg cgactggtcg gcgacgtatt
     8401 cggcgaccgc ggtcacctgg acgcggtaag gcgtggtctc gctcatcgtc ttttgttctc
     8461 ggtggggaag aggggcaggc gttaaaataa cagtttttcc cggaagctcc cgccatgtcg
     8521 aacgccgccc cgtccccgct caccgccctg tctccgctcg atggtcgcta ccatggcaag
     8581 gtcgccggcc tgcgcgaaca cttttcggag cacggcctga ttcgcaaccg cgtgcgcgtc
     8641 gaggtcgaat ggctgaaggc actcgccgcc gagccggcac tggccgagat tgccccgttc
     8701 tcgccggcca cggtggtgga actcgacgcc gtggtggcgc agttctcgac cgacgacggc
     8761 gaagcggtga aggcgatcga ggccaccacc aaccacgacg tcaaggccat ggagtactgg
     8821 ctcaagaagc gcctcggcca caacgccgag gtgatgaagg tgtccgagtt catccacttc
     8881 gcctgcacct ccgaagacat caacaacacc tcgcacgcgc tgatgctggc cgaaggccgc
     8941 gaccgtgtgc tgctgccggc gctcgacgcg gtgatcgccc ggttccgcga gttggcccac
     9001 gcgctcgccg acctgccgat gctgtcgcgc acccacggcc agcccgccag cccgaccacg
     9061 ctcggcaagg agatggccaa catcgcggcg cgcctgatgc gggcgcgcgc agccatcgcc
     9121 ggcgtggcga tgaccgccaa gttcaacggc gcggtcggca actacaacgc ccacctgtcg
     9181 gcttggccgc aattcgactg ggagggcttc aaccgccgct tcatcgagtc cctggggctg
     9241 accttcaacg aatacaccat ccagatcgag ccgcacgacg ccatggccga gctcttcgac
     9301 gccatcgccc gtgccaacac catgctgatc gacgcctgcc gcgacatctg gatgtacatc
     9361 tcgctcggct acttcaagca gaagctcaag gaaggcgagg tcggctcctc gaccatgccg
     9421 cacaaggtca acccgatcga cttcgagaat gccgagggca acttcggcct cgccaacgcg
     9481 gtgctcaagc acttctccga gaagctgccg atctcgcgca tgcagcgcga cctcaccgac
     9541 tcgaccgtgc tgcgcaacat gggtgtgggc ttcggccaca cggtgctcgc actcgacagt
     9601 tgcctgcgcg gactgggcaa gctcgaggcc gagcccgcgc gcctggcggc ggacctggat
     9661 gactgctggg aggtgctggc cgagccggtg cagaccgtga tgcgccgcta tggcatcgcc
     9721 aacccctacg agcagctcaa ggccatgacg cgcggcaagg gcatcacccg cgaggcgctg
     9781 caggaattca tccgcaccct ggccatcccc gccgaggcgc gtgaccacct gctggcgatg
     9841 acgccggcga gctacgtcgg caaggcggcg gagctcgccc gccgcatctg agcgtcgccc
     9901 gaatccgccg gccgcgcgcc ggcgcccatc ccgacgccac ccttgcggtg gcgttttcaa
     9961 tccaggagaa accctatggc cttcgccgac aacctcaaga ccctgcccgg cgtgtctcac
    10021 ctcgccgcgt tgaatctgat cgacgccgca gacgcggtcg tcgccaccat cgagaacaag
    10081 cccggccagg ccggctcgct ggcggtctac aaccacctcg cccagctcta cggcgcgatc
    10141 agccccgagg cggcgaagaa gggtctggag ctctatgccg agcacaccga ggatgcccgc
    10201 gccaaccccg gcaagcatcc caacatcgac cgcctgatcg ggctgatcga gcgtggggaa
    10261 acgctgcggg tgaagcaggt gttcgcggtg tgaggttccg catggtgtga tcgcctgcgc
    10321 ggcggcggcg gcggcggcgg cgacgcaaac tcgtcgctgc gctcctcggg catgcgtcgc
    10381 cttgtttccc ccgcgacgcc ctccacctcg gcgctcctga agttggcgcc cgccgccccg
    10441 agccccgacc cgcagccttg cgttcggggg tgtcgcaggc tggcgttttc cagttgcgcc
    10501 gcgttcgcag ggacggaggc gttgccgagg cgctgattcc cacaggcaag aaaaaagcgc
    10561 cgcgcgggcg ccttttccgg tcgggaagcg cgcagcagcc tcagaccacc gcctcttcct
    10621 tctccttctt cggcttgatg aactgctcgc gcgacacgcc cagccacatc accagcgggc
    10681 tcgccaccag caccgaggag tagatgccga agcagatgcc gattgtcagc gcgagcgaga
    10741 agtagtacag cgtttcgccg ccgaagacga gcatcgacag taccatcacc tgcgtgctgc
    10801 cgtgggtgat cacggtgcgc gagatggtgc tcgtgatcgc gtggtcgagc acctgtggcg
    10861 tggtcatgtt gcgcttcttc ttgaaggtct cgcgcacgcg gtcgaacacc accaccgact
    10921 cgttcaccga gtagcccagc accgccagca ccgccgccag caccgggagc gagaactccc
    10981 actggaagaa ggcgaagaag cccaggatga tgatcacgtc gtgcaggttg gcgatgatcg
    11041 ccgacaccgc gaagcgccac tcgaagcgca gcgccaggta gatcatgatg ccgacgatca
    11101 ccagcagcag cgccatcgag ccgtcggcgg cgagctcctt gccgacctg
//
LOCUS       DAOQNN010000059        11032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2746_length_11032_cov_10.059033, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000059 DAOQNN010000000
VERSION     DAOQNN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11032
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2746_length_11032_cov_10.059033"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..76)
                     /locus_tag="PKV97_05365"
     tRNA            complement(1..76)
                     /locus_tag="PKV97_05365"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Thr,seq:cgt)
     gene            412..1194
                     /locus_tag="PKV97_05370"
     CDS             412..1194
                     /locus_tag="PKV97_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB05342.1"
                     /translation="MSGLTLFEIALIGAAAFAAGAVNSVAGGGTFFSFPALLAIGVPP
                     VVANASNSVSLWPGSLAGAWAFRRELKRFSRSLPMLSVIAFVGGIGGGLLLLATSNAA
                     FAQLIPWLLLLATVLFAFSAQISALVKKWKPSPEGVDERHIGPGGYVFQLAVSLYGGF
                     FGAGMGILMIAALAIQGFKDVHEINALKNWLSAVIYSVAVATFVIADAVSWPHTLVML
                     VTATIGGYWGAAVARKLPALWLRRFIIAVGGVLTVYYFGKTL"
     gene            1203..2186
                     /locus_tag="PKV97_05375"
     CDS             1203..2186
                     /locus_tag="PKV97_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB05343.1"
                     /translation="MLAGLSAALGAGLLWGLVFLTPLLLADYPPFMLAIGRYLAFGVL
                     ALGLAWFDRAALARLTRADWIEAVKLALVGNLVYYATLASAIQLAGGPVPTLIIGTLP
                     VVIAITANLLDRHHGGGEDSAPWIRLAAPLALILGGLFAVHSASAHALPADASADYLL
                     GIGFGLCALACWTWYPIRNSRWLRARGAGLSRAWATAQGLATLPVAIVAGIGYWLWDT
                     RLAPGGAGFAWPLGPRPEAFVGMCILLGLAASWLGTLLWNRASHLLPAALVGQLIVFE
                     TLAALLYAFLWYGRWPGMAESAGIALLIAGVLGGVRVFRPGAARPAVDGTA"
     gene            2289..3224
                     /locus_tag="PKV97_05380"
     CDS             2289..3224
                     /locus_tag="PKV97_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNB05344.1"
                     /translation="MDQLTRIILEAGRSGVELSLFVLLPIMVVMLSLMRLLEARGVLD
                     WVVARLAPLLRPLGLTGLGVFAALQINLVSFAAPVATLTMMEGRGASDRHLAATLAMV
                     MAMAQANVTFPMASMGLAFGPALVLSVLGGLAAAAVTYHGFGRGLSAAEAPLDETLHH
                     RVADDAKGVLDVINRAGAEAFKISVGAIPMLVLALVAVMALRASGGIDALTALLAPLL
                     RAFELDPALILLTLTKYIAGGTAMMGVMDEMLKAGTTTVATLNASAGFLIHPFDVAGV
                     AVLISAGRRVAAVWRPAALGAMVGIAVRAVGHALL"
     gene            3309..3674
                     /locus_tag="PKV97_05385"
     CDS             3309..3674
                     /locus_tag="PKV97_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HNB05345.1"
                     /translation="MIAALTLLLVFQLVGEVIARALALPIPGPVIGMALLFLALLVRG
                     GPGEDLRQTSGQLLQHLSLLFVPAGTGIVIYGDRIAAEWLPLVVALFLSTFLALAVTA
                     LVLQALTHKRPKPTTEAGK"
     gene            3671..4405
                     /locus_tag="PKV97_05390"
     CDS             3671..4405
                     /locus_tag="PKV97_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HNB05346.1"
                     /translation="MNPNLEEIWVYLSTSPLLGLTLTLLAYQAAFWIYKRVGFHPIAN
                     PVLISVAILASLLLATGTPYQRYFDGAQFVHFLLGPATVALAIPLHAQWPRLKSMAGP
                     LLIALVAGSLTAALSAWGIGALLGGSRESLMSLAPKSVTTPIAMGVAERIGGLPSLTA
                     VLVISAGIIGAVAGRSVLNRLHIGDHAVRGFAIGIASHGIGTARAFQVSEQAGAFAAL
                     AMGLNGLITAVSLPWIMPWVERLFAS"
     gene            4478..6103
                     /locus_tag="PKV97_05395"
     CDS             4478..6103
                     /locus_tag="PKV97_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05347.1"
                     /translation="MLHAIYRWFDRNLLELGRELRLSYLPPLMVYCAAGVSGLTGIVG
                     TFFVKDYLGLSAAFLAALGFWAGIPWALKMPIGHLVDLLWRYKAGLVYLGATLIAASL
                     LVMIGLIGNPDAMRAVMPIEAWFVLSVLLSPVGYVMQDAVADAMTVEAVPRLDARGEP
                     IPPEAVRLMHTTMQMLGRVAIIGGTVLVSLANVVMFQGSEALPEAEKVAIYLRIYELA
                     LIIPLLSVSGVLLGGFLKRREAKRLAALGRSRADIDRLLHDPEEKTTPNGWILGGSAV
                     FVALTITVGLSGIEYGQEIIFAASFTIIGFMISRLLRELSPEAARTLLGTVIIIFAFR
                     ALPGPGAGASWWMIDELGFDQSFLSRLDLITSALTLAGLFLFRRFMAEKSIAQIVIFL
                     TVATTALSLPIIGMFHGLHEWTAAHTGGVVDARFIAIANTALESPLGQVSMVPMLAWI
                     ANSAPPHLKATFFAVMASFTNLALSASQLGTKYLNQLYTIAREVKDAATGAITTPADY
                     GELGALLVSVTVIALVLPLAAILLTRLAGLRSA"
     gene            6517..8565
                     /gene="fusA"
                     /locus_tag="PKV97_05400"
     CDS             6517..8565
                     /gene="fusA"
                     /locus_tag="PKV97_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNB05348.1"
                     /translation="MTPRSVHDIRNLALFGHAGSGKTTLVEALLAAAGEIGAPGSVER
                     GDTVADFDPQEKAMGHSLHAALVHLEWAGHWINLLDTPGLPDLCGRALVALPAADTAA
                     VVINAAAGIESGTRRMMDAAKGKCRMIVVNKIDAPGADLGALMDAIAATFGRECLPIN
                     LPAADGSRVIDCFFAPDGSAATAFSSPSAAHDAIVDQVVELDEALMAQYLEQGESLDP
                     EQLHAPFEQALREGHLVPVCFVSARSGAGVRELLDILGKLAPDPTEANVPAFLKGEGT
                     AAQAVEVALDAERHVVAHVFQIANDPYRGKLGLFRVHQGQVTPNSQLYIGDGRKPFKV
                     AHLLRMQGKNTVETQLAVPGDLCAVARVDELTRDAVLHDSHDEDYYHLAPAAWPQPVF
                     GLALMTRKPADEQKLSEALARLMEEDPCLEVSFDPRNRRTVVRGLGEQHLKIVLEELS
                     TRWNLQLDTATPSIPYRETITVVAEARYRHKKQSGGAGQFGEVALRVEPLPRGAGIEL
                     GDEVKGGTIPTNFMPAVEKGVRQALAEGALAGFPLQDLRVVVTDGKHHAVDSNEVSFV
                     TAGRHALLEAVLGARPILLEPLLDVQVKVADALFGEVSAELAGRRGRLTATDSPAPGW
                     TLISARVPMAALEGFESRLKAICAGESEFVLEAGGHEPAPQDVQAELARVFSNGNGNH
                     "
     gene            8621..8881
                     /locus_tag="PKV97_05405"
     CDS             8621..8881
                     /locus_tag="PKV97_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HNB05349.1"
                     /translation="MELDERQRAYWRSNLRLTFTLLGVWFGVTFVGGYFASALNTVSF
                     LGFPLGFYLFAQGALLVYLAIIVIYVLAMNRLDRRYRFNSRH"
     gene            complement(8917..9300)
                     /locus_tag="PKV97_05410"
     CDS             complement(8917..9300)
                     /locus_tag="PKV97_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05350.1"
                     /translation="MILNASIALNWILFLALFPIAFFWFRRAWRILFRRDFSEVALKG
                     GMPPPNPERYAPYTLAINLIGGGVIVFVILGIVTGSMDFDTWTASAGVTIWMKFFLDF
                     ALGRHAHPIVPRRKKVEQGKPSAEH"
     gene            complement(9452..>11032)
                     /locus_tag="PKV97_05415"
     CDS             complement(9452..>11032)
                     /locus_tag="PKV97_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNB05351.1"
                     /translation="PYLRKFGKFTVPQFIGDRFYSKTASTVAVVCLLTASLTYIIGQM
                     TGVGVAFSRFLGVSSDTGIYIGQAIVFLYAVFGGMKGITYTQVAQYIVLIAAYLVPAF
                     FISLQLTGNPLPQLGLGSTMAGTDVSLLAKLDLIVKDLGFDQYTTSIPGSTLNMFMYT
                     LSLMIGTAGLPHVIMRFFTVPTVKDARSSAGWALIFIALLYTTAPAVGAMAKLNLHGT
                     VNSAVGLETNAAGKLAVNPATVKANADLYATESSLQYEQRPEWMKRWEKTGLLKFEDK
                     NGDGRIQYYNDKTKDADAKAKAAAAGWKGNELTVNADIMVMANPEIALLPNWVIALVA
                     AGGLAAALSTAAGLLMAISSAVSHDLVKNVFAPSISDKNELLVGKISMAVAIVISGYL
                     GLNPPGFAAGTVALAFGIAASSLFPAIMMGIFSKKMNKEGAIAGMLAGLFVTLFYVFA
                     HKGIFFVKGTEFIDLIGGPNSFFGITPEAFGAVGAMVNFIVAVVVDKVTKEPPEHIQH
                     MVESVRIPRGSKLVDGAH"
BASE COUNT         1602 a         3968 c         3668 g         1794 t
ORIGIN      
        1 tggtgccgga gaagagactc gaactcccga ccttcgcatt acgaatgcgc tgctctacca
       61 actgagctac accggccaag ggcgcgcatc ttaacccagc gcgaagggcg cggcaacacc
      121 gcatgcggcc cgccggggcg ggcggatcac cgggggaggt ggcggcagac gcgagggcgg
      181 gaagcatcgg ggagaacccg tattcgatag aaaattcttc tcccacacct acattttgct
      241 atttgcctcc attagaatgc gcgccaattc agggcggagg agaccagccc gagcaagaac
      301 gcagcgccac gggggtgcgc ccccgtggca gccgcgccat ttgcgggccg gccgcactcc
      361 ggcacggacc acgcctgtcg acccagccct ccccgacgtc ccgccctcca catgtccggc
      421 ctcaccctct tcgagatcgc cctcatcggg gccgccgcat tcgccgcagg cgcggtgaac
      481 tcggtcgccg gcggcggcac cttcttctcc ttccccgcct tgctcgcgat cggcgtgccg
      541 ccggtggtgg ccaacgcgag caactcggtc tcgctgtggc cgggcagcct tgccggcgca
      601 tgggcgttcc ggcgcgagct caagcgcttc tcgcgcagcc tgccgatgct ctcggtgatc
      661 gccttcgtcg gcggcatcgg cggcggcctg ctgctgctgg ccacttccaa tgccgcgttc
      721 gcgcagttga tcccctggtt gctgctgctt gccaccgtat tgttcgcctt cagcgcgcag
      781 atctccgcgc tggtgaaaaa atggaagcca tctccggaag gggtcgacga acgccatatc
      841 ggcccgggcg gctacgtatt ccagctcgcg gtttcccttt atggcggctt cttcggcgcc
      901 ggcatgggca tcctgatgat cgccgcgctc gccatccagg gcttcaagga cgtgcacgag
      961 atcaacgcgc tgaagaactg gctgtctgcg gtgatctaca gcgtcgcggt ggcgaccttc
     1021 gtgatcgccg acgcggtgtc gtggccgcac acgctggtca tgctggtgac cgcgacgatc
     1081 ggcggctact ggggtgcggc ggtggcgcgc aagctgccgg cgctgtggct gcgccgcttc
     1141 atcatcgcgg tcggcggcgt gctcaccgtc tactatttcg gcaagacgct ctgagcgcgc
     1201 cgatgctcgc cggtctctcc gccgcgctcg gcgcgggcct gctctggggc ctggtgttcc
     1261 tcaccccgct gctgctcgcc gactacccgc ccttcatgct cgcgatcggg cgctacctcg
     1321 ccttcggcgt gctcgcgctc gggctggcgt ggttcgatcg cgcggcgctc gcccgcctca
     1381 cgcgcgcgga ctggatcgag gcggtcaagc tcgcgctggt cggcaacctg gtctactacg
     1441 ccacgctggc gagcgcgatc cagctcgccg gcgggccggt gccgaccctg atcatcggca
     1501 ccctgccagt ggtgatcgcg atcaccgcga acctgctcga ccgccaccac ggcggcggcg
     1561 aggacagcgc gccgtggatc cgcctcgcag caccgctggc gctcatcctc ggcggcctgt
     1621 tcgcggtgca ttcggcgagc gcgcacgcac tgcccgccga cgcctccgcc gactacctgc
     1681 tgggcatcgg cttcgggctg tgcgcgctcg cctgctggac ctggtatccg atccgcaaca
     1741 gccgctggct gcgcgcacgc ggtgccgggc tgtcgcgcgc atgggcgacc gcgcaggggc
     1801 tggcgacgct gccggtggcg atcgtcgccg gcatcggcta ctggctgtgg gacacccggc
     1861 tcgcgcccgg cggagcgggc ttcgcctggc cgctcgggcc gcgccccgag gccttcgtcg
     1921 gcatgtgcat cctgctcggc ctggccgcgt cctggctcgg caccctgctg tggaaccgcg
     1981 ccagccacct gctgccggcc gcactggtgg gccagctgat cgtgttcgag acgctcgccg
     2041 cgctgctcta cgccttcctg tggtacggac gctggccggg catggccgag agcgcgggga
     2101 tcgcgctgct gatcgccggg gtgctcggcg gcgtgcgggt gttccgcccg ggcgctgcac
     2161 gccccgcagt ggacgggact gcgtagcgga gcatcggcgg tggccgtgct gccggcttgc
     2221 acgggcgtcc cgccggacac ggtatcattc tgttttttgc actgcagcaa accgcttgcc
     2281 caccgccgat ggaccaactc acccgcatca tcctcgaggc cggccgctcg ggggtcgagc
     2341 tctccctctt cgtgctgctg ccgatcatgg tggtgatgct gtcgctgatg cgcctgctcg
     2401 aggcgcgcgg tgtgctcgac tgggtggtcg cccgcctggc gccgctgctg cgcccgctcg
     2461 ggctcaccgg gctgggcgtg ttcgccgcgc tgcagatcaa cctggtgagc ttcgccgcgc
     2521 cggtggccac gctcaccatg atggaagggc gcggcgcgtc cgaccgccac ctcgccgcca
     2581 cgctggccat ggtgatggcg atggcgcagg ccaacgtgac tttcccgatg gcctcgatgg
     2641 gactcgcctt cgggccggcg ctggtgctgt cggtgctcgg cggactggcg gccgccgcgg
     2701 tgacctacca tggcttcggc cgcggcctgt cggcggccga agcgccactg gacgagaccc
     2761 tgcaccaccg cgtcgccgac gacgccaagg gtgtgctcga cgtcatcaac cgcgccggcg
     2821 ccgaggcctt caagatctcg gtcggcgcga tcccgatgct ggtgctcgcg ctggtggcgg
     2881 tgatggcgct gcgcgcaagc ggcggcatcg acgcgctcac cgcgctgctc gccccgctgc
     2941 tgcgcgcgtt cgagctcgac cccgcgctga tcctgctcac gctcaccaag tacatcgctg
     3001 gcggcaccgc gatgatgggc gtgatggacg agatgctgaa ggcgggcacg accaccgtcg
     3061 ccaccctgaa cgcgagtgcc ggcttcctga tccacccctt cgacgtcgcc ggcgtggcgg
     3121 tactgatctc ggccggccgg cgcgtggcgg cggtgtggag gccggccgcg ctcggcgcga
     3181 tggtcggcat cgcggtgcgc gcggtcggcc acgccctgct gtaagctcgt ttcgcctgcc
     3241 tcctccggcc cccgtccggc gcccccgttc ggcgccccct tccagtgact tcgcgaggga
     3301 ctccgaccat gatcgccgcc ctgaccctgc ttctcgtctt ccagctcgtc ggcgaggtga
     3361 tcgcgcgcgc gctggcgctg ccgattcccg gcccggtgat cggcatggcc ctgctcttcc
     3421 tcgcgctgct cgtgcgcggc ggtccgggcg aggatctgcg ccagacctcc ggccagttgc
     3481 tgcagcacct gtcgctgctg ttcgtgccgg cgggcaccgg catcgtcatc tacggcgacc
     3541 gcatcgccgc cgaatggctg ccgctggtgg tggcgctgtt cctcagcacc ttcctcgcac
     3601 tggcggtgac cgcgctggtg ctccaggcac tcacccacaa gcgcccgaag ccgacgacgg
     3661 aggccggcaa atgaacccga acctcgagga aatctgggtc tatctgtcca cctcgccact
     3721 cctcgggctg acgctgaccc tgctcgccta ccaggctgca ttctggatct acaagcgcgt
     3781 cggcttccat ccgatcgcca acccggtgct gatcagcgtg gccatcctcg ccagcctgct
     3841 gctcgccacc ggtacgccct accagcgcta tttcgacggc gcgcagttcg tgcacttcct
     3901 gctcggcccg gccacggtcg cactggcgat cccgctccac gcgcagtggc cgcgcctgaa
     3961 gtcgatggcc ggcccgttgc tgatcgcgct cgtcgccggc tcgctgaccg cggcgctgtc
     4021 ggcctggggc atcggcgccc tgctcggcgg cagccgcgaa tcgctgatgt cgctcgcccc
     4081 caagagcgtg accacgccga tcgccatggg cgtggccgag cgcatcggcg gcctgccctc
     4141 gctcaccgcg gtgctggtga tcagcgccgg catcatcggc gcggtggccg gccgcagcgt
     4201 gctgaatcgc ctgcacatcg gcgatcacgc cgtgcgcggc ttcgcgatcg gcatcgcctc
     4261 gcacggcatc ggcaccgcgc gcgccttcca ggtcagcgag caggcgggcg ccttcgccgc
     4321 gctggcgatg ggattgaacg gactgatcac cgcggtgtcc ctgccctgga tcatgccctg
     4381 ggtggagcgg ctgttcgcga gctgacggct gtccggggcg cgcaagacgc cagaacaaac
     4441 agaatccgac acgacccgac gacgaggggc cgaccaagtg ctgcacgcca tctaccgctg
     4501 gttcgaccgc aacctgctcg agctcggccg cgagctgcgg ctctcctacc tgccgccctt
     4561 gatggtgtat tgcgcggcgg gcgtctcggg cctcaccggc atcgtcggca ccttcttcgt
     4621 caaggactac ctcggcctgt cggccgcctt cctcgccgca ctcggcttct gggccggtat
     4681 cccgtgggcg ctgaagatgc cgatcgggca tctggtggac ctgctgtggc gctacaaggc
     4741 cgggctggtc tatctgggcg ccacgctgat cgccgccagc ctgctggtca tgatcgggct
     4801 catcggcaac ccggatgcga tgcgcgcggt gatgccgatc gaggcctggt tcgtgctgtc
     4861 ggtgctgctg tcgccggtgg gctacgtgat gcaggacgcg gtggccgacg ccatgacggt
     4921 cgaggccgtg ccgcggctgg acgcgcgcgg cgagccgatc ccgccggaag cagtccgcct
     4981 gatgcacacc accatgcaga tgctcggccg ggtggcgatc atcggcggca cggtgctggt
     5041 gtcgctcgcc aacgtggtga tgttccaggg ctcggaggcc ctgcccgagg cagagaaggt
     5101 ggcgatctac ctgcgcatct acgagctcgc gctgatcatc ccgctgctgt cggtgagcgg
     5161 cgtgctgctc ggcggcttcc tcaagcgccg cgaggcgaag cggctggccg cgctcgggcg
     5221 cagccgcgcc gacatcgacc gcctgctcca cgatccggaa gagaagacca cgcccaacgg
     5281 gtggatcctc ggcggcagcg cggtgttcgt ggcgctgacc atcactgtcg ggctgtccgg
     5341 catcgaatac ggccaggaga tcatcttcgc ggcctcgttc accatcatcg gcttcatgat
     5401 cagccgcctg ctgcgcgagc tctcgcccga ggccgcgcgc accctgctcg gcacggtgat
     5461 catcatcttc gccttccgcg ccctgcccgg cccgggcgcg ggcgcgagct ggtggatgat
     5521 cgacgagctc ggcttcgacc agagcttcct gtcgcggctc gacctcatca ccagcgcgct
     5581 gacgctcgcc ggcctgttcc tcttccgccg cttcatggcc gagaagtcga tcgcgcagat
     5641 cgtgatcttc ctcaccgtcg ccaccaccgc gctgtcgctg ccgatcatcg gcatgttcca
     5701 cggcctgcac gaatggaccg cggcgcacac cggcggcgtg gtggacgcgc gcttcatcgc
     5761 catcgccaac accgcgctcg agtcgccgct cggccaggtg tcgatggtgc cgatgctggc
     5821 ctggatcgcg aactcggcgc cgccgcacct gaaggcgacc ttcttcgcgg tgatggcctc
     5881 cttcaccaac ctcgcgctgt cggcctcgca gctcggcacc aagtacctca accagctcta
     5941 cacgatcgcg cgcgaggtca aggacgccgc caccggggcg atcaccacgc cggccgacta
     6001 tggcgagctc ggcgcgctgc tggtctcggt gaccgtgatc gcgctcgtgc tgccgttggc
     6061 agccatcctc ctcacccgcc tcgccggcct gcgcagcgcc tgagcccccg cgcgggccgg
     6121 aaggccggag atcgcgactc gcgtcgctcc tacggcgccg ttccggaaac gcggtggcct
     6181 tcgtacgagc gtcgcgagtc gcgatcacgg cgcagccccg gaagcgcagc cgctctcgtg
     6241 agagcgacgc aagtcgcgat cgcagcacca ccccggaagc gcagccgctc tcgtgagagc
     6301 gacgcaagtc gcgatcgcag caccgccccg gaagcgcagc cgctcccgtg ggagcgacgc
     6361 aagtcgcgat acaccgtagc tcgaacgcga acgatcccgc gcgatcggtt cgctacgccg
     6421 gctcccgccg actgcgatcg ccccggcgga ttgccttcgc accgcgacag gcacagactt
     6481 gagggcatcc gcattcaatc gcaagggagg cacacgatga caccccgctc ggttcacgac
     6541 atccgcaacc tggccctctt cggccacgcc ggcagcggca agaccaccct cgtcgaggcc
     6601 ttgctcgccg ccgccggcga gatcggcgcg cccggcagcg tggagcgcgg cgacacggtc
     6661 gcggacttcg acccgcagga aaaagcgatg ggccactcgc tgcatgcggc gctcgttcac
     6721 ctggagtggg cgggccactg gatcaacctg ctcgacaccc cgggcctgcc cgacctgtgc
     6781 ggccgcgccc tggtcgcgct acccgcggcc gacaccgccg cggtggtgat caacgccgcg
     6841 gccggcatcg agagcggcac ccggcgcatg atggacgccg cgaagggcaa gtgccgcatg
     6901 atcgtggtca acaagatcga cgccccgggc gccgacctcg gcgcgctgat ggacgccatc
     6961 gccgccacct tcggccgcga gtgcctgccg atcaacctgc ccgcagccga cggcagccgc
     7021 gtcatcgact gcttcttcgc gccggacggc agcgccgcca ccgccttctc cagcccctcg
     7081 gccgcccacg acgcgatcgt cgaccaggtc gtcgagctcg acgaggcgct gatggcgcag
     7141 tacctcgagc agggcgagtc gctcgacccc gagcagctcc acgccccctt cgagcaggcc
     7201 ctgcgcgagg gccacctggt gccggtgtgc ttcgtctccg cgcgcagcgg cgccggcgtg
     7261 cgcgagctgc tcgacatcct cggcaagctc gcccccgacc ccaccgaggc caacgtgccc
     7321 gccttcctca agggcgaggg cacggcggcg caagcggtcg aggtcgcgct cgatgccgag
     7381 cgccacgtcg tcgcccatgt cttccagatc gccaacgacc cctaccgcgg caagctcggc
     7441 ctgttccggg tgcatcaggg ccaggtcacg cccaactcgc agctctacat cggcgacggc
     7501 cgcaagccct tcaaggtcgc ccacctgctg cgcatgcagg gcaagaacac ggtcgagacc
     7561 caactcgcgg tgcccggcga cctgtgcgcg gtggcgcgcg tcgacgagct gacccgcgac
     7621 gcggtgctgc acgactcgca cgacgaggac tactaccacc tcgcccccgc cgcctggccg
     7681 cagccggtgt tcggcctcgc gctgatgaca cgcaagccgg ccgatgagca gaagctctcc
     7741 gaggcgctcg cccgcctgat ggaagaggac ccctgcctcg aggtcagctt cgatccgcgc
     7801 aaccgccgca ccgtggtgcg cggcctcggc gagcagcacc tcaagatcgt gctcgaggag
     7861 ctttccacgc gctggaacct gcagctcgac accgccacgc cgagcatccc ctaccgcgag
     7921 acgatcaccg tggtggccga ggcacgctac cgccacaaga agcagagcgg cggcgccggc
     7981 cagttcggcg aggtcgcgct gcgcgtcgag ccgctgccgc gcggcgccgg catcgaactg
     8041 ggcgacgagg tcaagggcgg cacgatcccg accaacttca tgcccgcggt cgagaagggc
     8101 gtgcgccagg cgctcgccga gggcgcgctc gcgggcttcc cgctgcagga cctgcgcgtc
     8161 gtggtcaccg atggcaagca tcacgcggtc gactcgaacg aggtctcctt cgtcaccgcc
     8221 ggccgccatg ccttgctcga agccgtgctg ggcgcgcgtc cgatcctgct cgagcccctg
     8281 ctcgacgtgc aggtgaaggt ggcggatgcg ctcttcggcg aggtgagcgc ggagctcgcc
     8341 ggccgccgcg ggcgcctgac cgcgaccgac agcccggcgc cggggtggac gctgatctcg
     8401 gcgcgcgtgc cgatggctgc gctggaaggc ttcgagagcc ggctgaaggc gatctgcgcc
     8461 ggcgagagcg agttcgtgct cgaggccggc ggccacgagc cggcaccgca ggacgtccag
     8521 gccgagctgg cgcgggtgtt ctccaacggc aacggcaacc actaagaagg cccgcagcgc
     8581 ccgactccgc gaggggtcgg gcgggccgcc tgcgactgga atggaactcg acgaacgcca
     8641 gcgcgcctac tggcgcagca atctccgcct caccttcacg ctgctcggcg tgtggttcgg
     8701 cgtgaccttc gtcggaggat atttcgccag cgcgctcaac acggtgagct ttctcggctt
     8761 tccgctgggt ttttatctgt tcgcccaagg ggcgctgctg gtgtatctcg cgatcatcgt
     8821 gatctacgtg ctcgccatga accggctcga ccggcgctac cgcttcaaca gccggcacta
     8881 aaggccggcg cccgctcgca gggcatgcgg cgtacttcag tgctctgcag agggcttgcc
     8941 ttgctcgacc ttcttgcgcc gcggcacgat cgggtgcgca tgccggccga gcgcgaaatc
     9001 caggaagaac ttcatccaga tcgtcacccc cgcgctcgcg gtccaggtgt cgaagtccat
     9061 gctgccggtc acgatgccga ggatcacgaa gacgatcacc ccaccgccga tcaggttgat
     9121 cgcaagcgtg tagggcgcat agcgctccgg gttcggcggc ggcatgccgc ccttcagcgc
     9181 gacctcggag aaatcccgcc tgaacaggat ccgccacgcc cgccggaacc agaagaaggc
     9241 gatcgggaac agggcgagga acagtatcca gttgagggcg atgcttgcgt tgaggatcat
     9301 tctcgactcc agcatgggcg gcgagtcgat cgccgtcctg cacggtacca ctttccggaa
     9361 ataaaaagcc ccgccaatcc gaccggcggg gctccgtccg caccatccgg cgcgcgccgg
     9421 atggtgctgc ccgacagttg ccggacgctg cttagtgggc gccgtccacc agcttcgaac
     9481 cgcgcgggat acgcaccgac tcgaccatgt gctggatgtg ctccggcggc tccttggtca
     9541 ccttgtcgac cacgaccgcg acgatgaagt tcaccatcgc acccaccgca ccgaaggctt
     9601 ccggcgtgat gccgaagaag ctgttcgggc cgccgatcag gtcgatgaac tcggtgccct
     9661 tcacgaagaa gatgcccttg tgcgcgaaca cgtagaacag cgtgacgaag aggccggcca
     9721 gcatgcccgc gatcgcgcct tccttgttca tcttcttgga gaagatgccc atcatgatcg
     9781 ccgggaacag cgacgaggcg gcgataccga aggccagcgc gaccgtaccg gcggcgaagc
     9841 ccggcgggtt gagaccgagg tagccggaga tcacgatcgc caccgccatc gagatcttgc
     9901 ccaccagcag ctcgttcttg tcgctgatgc tcggcgcaaa cacgttcttc accaggtcgt
     9961 gcgacaccgc ggacgagatc gccatcagca gaccggcggc ggtcgacaat gcggcagcca
    10021 gaccacccgc ggcgaccagc gcgatcaccc agttgggcag cagcgcgatc tcggggttcg
    10081 ccatcaccat gatgtcggcg ttcaccgtga gctcgttgcc cttccaaccc gcggccgcgg
    10141 ccttggcctt ggcatcggcg tccttggtct tgtcgttgta gtactggatg cggccgtcgc
    10201 cgttcttgtc ctcgaacttc agcagaccgg tcttctccca acgcttcatc cactcggggc
    10261 gctgctcgta ctgcaggctg gactcggtgg cgtacaggtc ggcgttggcc ttgaccgtgg
    10321 ccgggttcac cgccagcttg cctgcggcgt tggtctcgag accgaccgcc gagttcaccg
    10381 tgccgtgcag gttcagcttg gccatcgcgc cgaccgcagg tgcagtggtg tacagcaggg
    10441 cgatgaagat cagcgcccaa cccgccgagc tgcgcgcgtc cttcaccgtc ggaacggtga
    10501 agaagcgcat gatgacgtgc ggcaggcccg cggtgccgat catcagcgac agggtgtaca
    10561 tgaacatgtt gagcgtgctg cccgggatcg aggtcgtgta ctggtcgaag cccagatcct
    10621 tcacgatgag gtcgagcttg gcgagcagcg agacatcggt gccggccatg gtcgacccga
    10681 ggccgagctg cggcagcggg ttgccggtga gctgcagcga gatgaagaag gccggcacca
    10741 ggtaggcggc gatcagcacg atgtactgcg cgacctgggt gtaggtgatg cccttcatgc
    10801 cgccgaacac cgcgtacagg aacacgatcg cctggccgat gtagatgccg gtgtcgctcg
    10861 agacgccgag gaagcgcgag aaggccacgc cgacgccagt catctgaccg atgatgtagg
    10921 tcagcgacgc ggtcaacagg cacaccaccg ccaccgtcga agccgtcttc gagtagaagc
    10981 ggtcgccgat gaactgcggc acggtgaact tgccgaactt gcgcaggtac gg
//
LOCUS       DAOQNN010000060        11016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2754_length_11016_cov_8.468935, whole genome shotgun sequence.
ACCESSION   DAOQNN010000060 DAOQNN010000000
VERSION     DAOQNN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2754_length_11016_cov_8.468935"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PKV97_05420"
     CDS             complement(<1..813)
                     /locus_tag="PKV97_05420"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNB05352.1"
                     /translation="MSTPGAGRRGRIESSTPAAIAEAAALLRAGELVGMPTETVYGLA
                     ADALDPEAVKKIFAAKGRPADHPLIVHLPSAEHLPQWAAAIPKDALALARAFWPGPLT
                     LILKRTPDVPDEVTGGQDTVGVRVPAHPVALALLRAFDGGLAAPSANRFGRISPTTAA
                     HVHEELGERVAMILDGGACAVGIESTILDFSRDVPEILRPGAITPEDIARVIGRRPRV
                     PGESVAETASARAGQCGDASGLAEAAAPNAAADAAQPAPRVSGALAAHYAPRT"
     gene            complement(824..1990)
                     /locus_tag="PKV97_05425"
     CDS             complement(824..1990)
                     /locus_tag="PKV97_05425"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNB05353.1"
                     /translation="MILPPATLGMLGGGQLGRFFVSAAHEMGYKVWVLDPDPHSPAGL
                     VADRHLVAAYDDYVALDSLGNECAAVTTEFENVPADTLDYLTKFIPVHPAASAVAVCQ
                     NRIAEKTFLADNGLPHGPFAVVRSEDDVRAANAGLFPAILKVARFGYDGKGQARVADR
                     DEALAAFQHFKGEPCVLEKMLALDYEVSVVLARDEAGNVRAFPTGENRHRNGILDVTI
                     APARASAFPGEGTQLSAQEIAERIADKLGYVGTLGVEFFVCRGELVVNEMAPRPHNSG
                     HHTIDACDASQYEQQVRALCGLPLATPRQHSAAVMVNLLGELWYEDGKGHGAYREPDW
                     SVLHAVPGLRLHLYGKHHARPGRKMGHFVVIGDDAAVVLERALAARAAIGIRDE"
     gene            complement(2001..2492)
                     /gene="purE"
                     /locus_tag="PKV97_05430"
     CDS             complement(2001..2492)
                     /gene="purE"
                     /locus_tag="PKV97_05430"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNB05354.1"
                     /translation="MNETQPVVGIIMGSNSDWPTMKAAAKMLEDFGVPFEAKVVSAHR
                     TPDLMFEYAEAARSRGLKAIIAGAGGAAHLPGMVAAKTTLPVLGVPVQSKALSGQDSL
                     LSIVQMPKGIPVATFAIGEAGAANAGLFAVALLAVEDAVLAHKLADFRARQTQAVLDM
                     TLD"
     gene            complement(2503..3390)
                     /gene="folD"
                     /locus_tag="PKV97_05435"
     CDS             complement(2503..3390)
                     /gene="folD"
                     /locus_tag="PKV97_05435"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNB05355.1"
                     /translation="MTARIIDGNALSARVRGEIAERAAALTARGVQPCLAVMLVGADP
                     ASAVYVRNKVAGCEKAGIRSLRFDYPETATQAEVMAKLAELNADPAVHGILVQLPLPK
                     HLDETAVLEAIDLKKDVDGFHAENVGRLSQCREAFIPCTPHGVMKMLESSGTPVQGAE
                     AVVIGRSNIVGKPMATLLTNAGATVTVCHSRTKDLAFHTRRADILVAAIGKPRFVTGD
                     MIKPGATVIDVGINRLQDGPDAGKLCGDVDFESAREVAGAITPVPGGVGPMTITMLLE
                     NTVISAERAASDRKLAAGK"
     gene            complement(3491..5233)
                     /locus_tag="PKV97_05440"
     CDS             complement(3491..5233)
                     /locus_tag="PKV97_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNB05356.1"
                     /translation="MTGINALFLLTGLLLFISVLASTISARLGLPLLLLFLGVGMLAG
                     EDGPGGIVFEDFFIASLIAQAALSVILLDGGLRTRVSSFRVALRPAAVLASWGVIGTA
                     ALLGVFATWLLDVDWRLGMLLAAIVGSTDAAAVFALLRNSGVRLNERVQATLEIESGA
                     NDPMAILLVTAMVGTLLQPETATLGSFVWMLASQLGLGAVLGLAGGWVLSRLMMRLAL
                     PDGLYALLILSGGTLVFAATNLLNGSGFLAVYLAGIVVGNRRSHATEHVLRVMDGLAW
                     LAQAGMFVVLGLLVTPSKMIDHAAEALAMAVFLMLVARPLAVAIGLLPFRYPRNELLY
                     VSWVGLRGAVPVVLAIVPVVMGVPDSQLLFNVAFAVVLLSLLVQGATVPLAARWLGVE
                     VPPRDEPRDKREVWVGEAAAVDMLEFCVAAGAKADGRHPDELGTDHPGHDVRCVALVR
                     EGSLHRLTPDTRLRPGDSVWMIAPDEISEQLARTFAGSRGELTANASFFGEFVVDPDS
                     RASDLADAYGLRIAEAERELAMGELLAVRLGRPPVVGDRVDIGAFALTVREMGDKGRI
                     TALGLKCPPFGGGR"
     gene            complement(5379..6017)
                     /locus_tag="PKV97_05445"
     CDS             complement(5379..6017)
                     /locus_tag="PKV97_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB05357.1"
                     /translation="MSAVPAAPDQIVHVVDDDEALRDSLAWMLEANGHAVAVHESGEA
                     FLAACTPEMTGCVLLDVRMPGMSGLEVFEELGRRRCGLPVVFITGHGDVPMAVSALKQ
                     GAVDFVEKPFGEREMLRVVEQCLRLERETRDRRLQEADTARRLGLLTQREREVLELII
                     VGKLNKQIADVLGISIKTVEVHRARVMEKMGAHSLAELVQHVVVLEPGAVPR"
     gene            complement(5992..>7453)
                     /locus_tag="PKV97_05450"
     CDS             complement(5992..>7453)
                     /locus_tag="PKV97_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB05358.1"
                     /translation="VVTGLRAIDLSGRIIYVNSAFCRMIGFDEDELVGAGPPFPYWPQ
                     EQLAECERNLSMTLSGRAPRSGFEMRIRRKNGERFDARFYLSPLIDLTGRQTGWMASI
                     TDITEPKRVRAALESAHERFEAVLDGLDAAVFVADARTDEILFANLAFKRIHGFDAVG
                     RTVRGVAVPQPERGDYRVDPRNLSPADVPRELFDGELLHPQSGRWYHVREQATRWVDG
                     RVVRMGIATDITDRKQTAAVAREQEERLQRTSRLITMGEMASTLAHELNQPLAAIANY
                     CAGSVTRLQSGKGRTEDVLAAMQKASFQAERAGKIIRRVREFVKKSEPQRCAVDLVEV
                     LEDAIGFADIDAHRTRIRIHTELEPGLPPVYADRIMIEQVLLNLIRNGLDAMADALPE
                     ARVLTVRVRTVGADAVEVAVIDRGHGISEEGRAQLFTPFYTTKAEGMGMGLNICRSIV
                     EFHNGRLLVDANPEGGTIFTFTLPTESAIERSARSA"
     assembly_gap    7454..7553
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7554..8836)
                     /locus_tag="PKV97_05455"
     CDS             complement(<7554..8836)
                     /locus_tag="PKV97_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB05359.1"
                     /translation="MQASAAARATLSRWYWAMPYLAVTVLALSMLAVVWLLQARETAV
                     ERDALARDLQWTEQSIRRAMLTTEEFLVQLARDLSAGTLDHDDFQLRANEHLAINPAL
                     ANLAWVDREHVIAWSAPFDTRDLLAGEILVDDQVEAFQNSALSARLTYGRPYIDARGS
                     AVIEVYAPVLRRGDSLGAIVAVYSIERMLSRLVPARFAEKYRLAVLDRDGGVLTLSSA
                     LPPAQEALSLTLALDPPGNGLALQAMAFRPGGAVLRYLPAVLIVGLSLLVLWSLWTLR
                     RHVQRRVRVEKERDQLFDLSLDLLCVIDLDGRFGRCNPAFERVLGHDPRSLPGCSLID
                     FVHPEDVSETLAMLRRLAGGEPVRFETRCRCADGSYKWLMWSINPVREERLVYAVAHD
                     VTGRKATEDALRAESAFRKAMEDSVVTGLRAIDLSG"
     gene            9026..10273
                     /locus_tag="PKV97_05460"
     CDS             9026..10273
                     /locus_tag="PKV97_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB05360.1"
                     /translation="MKPFLALLGRAFVTLLLLAPQLSAQAVPAEAVGAGARPSGGERA
                     PIRVGVSGPFSGPSAPMGLSMREGIRIAAEELNAGGGLLGRRIELVERDDEASNELGA
                     QIVRDFIHRERVTAGLGIVNTGVALASQRHYQMARIPVITSVATGSLITKQFQPPDFT
                     ENYVFRVSASDTLQAAVIVEEAVGRRGLTRLAILHDATNYGVLGSQDLIAALGTRGRT
                     AVVVERFQLRETDMRPQLERARAAGAQAVLTYGIGPELAHIANSMARLGWQVPIIGSW
                     TLAMSSFIELAGRNAEGARMPQTFIAEARSPAQAAFLAAWERATGSVRIPVPPAAAQG
                     YDSMQLLAAAIRQAGSLDGPRIREALENLDAEVDGVIMRYRRPFSRDNHETLRSARQI
                     HLGEIRDGAVVFAHEATPGRPGP"
     gene            10457..>11016
                     /gene="aceE"
                     /locus_tag="PKV97_05465"
     CDS             10457..>11016
                     /gene="aceE"
                     /locus_tag="PKV97_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936832.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNB05361.1"
                     /translation="MTGISTVLPQTDTDAQETKEWLDALAAVVEHEGPERAHYLIETL
                     IATARQEGVNIPYSATTEYINTIPAERQPPYPGDPDLEIKIHSYIRWNAMAMVVRANK
                     DTNVGGHIASFASAAALYDVGFSHFWHAPSEAHDGDLIYFQGHSVPGVYARAFMLGRL
                     TEEQMDSFRQEVGGKGISSYPHPWLMP"
BASE COUNT         1712 a         3808 c         3860 g         1536 t
ORIGIN      
        1 ggtgcgcggg gcgtaatggg cggcgagtgc gccggagacg cgcggagcgg gctgcgctgc
       61 atcggctgcg gcgttggggg cggcggcttc cgcgagtccg gacgcgtcgc cgcactgccc
      121 tgcccgcgcg gaagcggttt ccgcgaccga ctcgcccggc acccgcggcc ggcggccgat
      181 gacgcgcgcg atgtcctcgg gcgtgatcgc gcccgggcgc aggatctccg gcacgtcgcg
      241 cgagaagtcg aggatggtgg attcgatgcc gaccgcgcag gcaccgccgt cgaggatcat
      301 cgccacgcgc tcgccgagtt cttcatgcac atgcgcggcg gtggtggggc tgatgcggcc
      361 gaagcggttg gcgctgggtg cggccagccc gccgtcgaag gcccgcagca gggcgagcgc
      421 gaccgggtgc gcgggcacgc gcacgccgac cgtgtcctgc ccgccggtga cttcgtcggg
      481 tacgtcgggt gtgcgcttca ggatcagcgt cagcgggccg ggccagaagg cgcgcgcgag
      541 cgcgagcgcg tccttgggga tggcggcggc ccactgcggc aggtgctcgg ccgagggcag
      601 gtgcacgatc agcgggtggt cggcgggacg gcccttggcg gcgaagattt tcttcaccgc
      661 ctcggggtcg agcgcgtcgg cggccagccc gtagaccgtc tcggtcggca tgccgacgag
      721 ttcgcccgca cgcagcaggg cagcagcctc ggcgatcgcg gcgggcgtgg aggattcgat
      781 gcggccgcgc cgtccggccc cgggggtact catcggccgg cgctcactcg tcgcggatgc
      841 cgatcgcggc acgcgcggcg agcgcgcgct cgagcaccac ggcggcatcg tcgccgatca
      901 ccacgaaatg gcccatcttg cgccccgggc gggcgtggtg cttgccgtag agatgcaggc
      961 gcaggccggg caccgcgtgc agcaccgacc agtcgggctc gcggtaggca ccatggccct
     1021 tgccatcctc gtaccacagc tcgccaagca gattcaccat caccgccgcc gaatgctggc
     1081 gcggagtggc cagcggcagg ccgcacagcg ccctcacctg ctgctcgtac tggctggcgt
     1141 cgcaggcgtc gatggtgtgg tggccgctgt tgtgcgggcg cggggccatc tcgttcacca
     1201 ccagctcgcc gcggcagacg aagaactcca cccccagcgt gccgacgtag ccgagcttgt
     1261 cggcgatgcg ctcggcgatc tcctgcgcgc tcagttgcgt accctcaccg gggaaggccg
     1321 acgcgcgcgc cggcgcgatg gtcacgtcga ggatgccgtt gcggtggcgg ttctcgccgg
     1381 tcgggaaggc gcgcacattg cccgcctcgt cgcgcgccag caccaccgac acctcgtagt
     1441 ccagcgccag catcttctcc agcacgcagg gctcgccctt gaagtgctgg aaggcggcga
     1501 gcgcctcgtc gcggtcggcg acgcgggcct ggcccttgcc gtcgtagccg aagcgggcca
     1561 ccttgaggat ggcggggaac agcccggcat tggctgcgcg cacgtcgtcc tcgctgcgga
     1621 cgaccgcgaa ggggccgtgc ggcaggccgt tgtcggcgag gaaggtcttt tcggcgatgc
     1681 ggttctggca caccgcgacc gcgctcgccg ccgggtgtac cgggatgaac ttggtcaggt
     1741 agtcgagcgt gtcggcgggg acgttctcga actcggtggt caccgcagcg cattcgttgc
     1801 ccaggctgtc gagggcgacg tagtcgtcgt aggcggcgac caggtggcgg tcggcgacga
     1861 ggccggcggg gctgtgcggg tcggggtcga gcacccagac cttgtagccc atctcgtgcg
     1921 cggccgaaac gaaaaagcgg ccgagctggc cgccgccgag catgcccagg gtggcaggcg
     1981 ggaggatcat gatgcgacgc tcaatccagg gtcatgtcga gcaccgcctg ggtctggcgg
     2041 gcacggaagt cggcgagctt gtgcgccagc acggcatcct cgaccgcgag cagcgccacc
     2101 gcgaagaggc ccgcgttggc cgcgcccgcc tcgccgatgg cgaaggtggc gaccgggatg
     2161 cccttgggca tctgcacgat ggagagcagc gagtcctgcc ccgacagcgc cttcgactgc
     2221 accggcacac cgagcaccgg cagcgtggtc ttggccgcga ccatgcccgg caggtgggcc
     2281 gcaccgccgg cgcccgcgat gatcgccttg agcccgcggc tgcgcgcggc ctcggcgtac
     2341 tcgaacatca ggtcgggggt gcggtgggcg gagacgacct tggcctcgaa gggcacgccg
     2401 aagtcttcca gcatcttcgc ggccgccttc atcgtgggcc agtcggagtt ggagcccatg
     2461 atgatgccga cgacgggttg ggtttcgttc atctcgcagt cctcacttgc cggccgcgag
     2521 cttgcgatcg ctcgcggcgc gttcggcgga aatcacggtg ttttccagca gcatggtgat
     2581 ggtcatcggc cccacgccgc ccggcaccgg ggtgatcgcg ccggcgactt ccctggccga
     2641 ctcgaaatcc acgtcgccac acagcttgcc ggcgtcgggg ccgtcctgga ggcgattgat
     2701 gccgacgtcg atcacggtgg cgcccggctt gatcatgtcg ccggtgacga agcgcggctt
     2761 gccgatcgcc gccaccagga tgtcggcgcg gcgggtgtgg aaggcgaggt ccttcgtcct
     2821 ggagtggcac acggtgacgg tggcgccggc gttggtcagc agcgtggcca tcggcttgcc
     2881 gacgatgttc gagcgcccga tcaccaccgc ctcggcgccc tgcaccggcg tgccgctgga
     2941 ctcgagcatc ttcatgacac cgtgcggcgt gcacgggatg aaggcctcgc ggcactgcga
     3001 caggcggccg acgttctcgg catggaaacc gtcgacgtcc ttcttgaggt cgatggcctc
     3061 gagcaccgcg gtctcgtcga gatgcttggg cagcggcagt tgcaccagga tgccatgcac
     3121 cgcggggtcg gcgttgagct cggcgagctt ggccatgacc tcggcctggg tggcggtctc
     3181 ggggtagtcg aagcgcagcg agcggatgcc ggccttctcg cagccggcga ccttgttgcg
     3241 cacgtagacc gccgaggccg ggtcggcgcc caccagcatc accgccaggc agggttgcac
     3301 gccacgcgcc gtgagcgcgg ccgcacgttc ggcgatttcg ccgcgcacgc gcgcggacag
     3361 cgcgttgccg tcgatgatgc gagcggtcat ccttcaagcc cccgaaaaaa accgcgattt
     3421 tacagcatcg cggcggcggc ggcccgtacg gcggattcgg gtcggcatcg tgcggccaat
     3481 gcggcccgcc tcaacgccct ccgccgaacg gcgggcactt gaggccgagg gcggtgatgc
     3541 ggcccttgtc gcccatctcg cgcaccgtga gcgcgaaggc accgatgtcg acgcggtcgc
     3601 cgaccactgg cgggcggccg aggcggacgg cgagcaactc gcccatcgcc agctcgcgct
     3661 cggcctccgc gatgcgcagg ccgtaggcgt cggcgaggtc gctcgcacgg ctgtcggggt
     3721 cgaccacgaa ctcgccgaag aagctggcgt tggcggtgag ttcgccgcga ctgccggcga
     3781 aagtgcgcgc gagctgttcg gagatttcgt cgggcgcgat catccacacc gagtcgccgg
     3841 gacgcaggcg ggtgtcgggg gtcaggcggt gcaggctgcc ctcgcgcacc aaggccacgc
     3901 agcgcacgtc gtggccggga tggtcggtgc cgagctcgtc gggatggcgc ccgtcggcct
     3961 tggcgccggc cgcgacgcag aactccagca tgtccaccgc cgccgcctcg cccacccaca
     4021 cctcgcgctt gtcacgcggc tcgtcgcgcg gcggcacctc gacacccagc cagcgcgccg
     4081 ccagcggcac ggtcgcgccc tgaaccagca aggacagcag caccaccgcg aaggcgacgt
     4141 taaacagcag ttgcgaatcg ggcacgccca tcaccaccgg cacgatcgcg agcaccaccg
     4201 gcaccgcgcc gcgcaggccc acccagctca cgtagaggag ctcgttgcgc ggatagcgga
     4261 agggcagcag cccgatcgcc accgccagcg gccgcgccac cagcatcagg aacaccgcca
     4321 tcgccagcgc ctcggcggca tggtcgatca tcttcgacgg tgtcaccagc agccccagca
     4381 cgacgaacat gcccgcctgg gcgagccagg ccaggccgtc catcacccgc aggacgtgct
     4441 cggtggcatg gctgcggcgg ttgccgacga cgatgcccgc gaggtacacg gcgagaaagc
     4501 cgctgccgtt cagcaggttg gtcgccgcga aaaccaaggt gccgccggac aggatcagca
     4561 acgcatacag cccgtcgggc agcgccagcc gcatcatcag ccgcgacagc acccagcccc
     4621 cggccaggcc gaggacggcc ccgagcccga gctggctcgc cagcatccag acgaaactgc
     4681 cgagcgtggc ggtctcgggc tgcagcagcg tgccgaccat ggcggtaacg agcaggatcg
     4741 ccatcggatc gttggcaccg gactcgatct ccagcgtggc ctggacgcgc tcgttgaggc
     4801 ggacgccgct gttgcgcagg agcgcgaaca ccgcggccgc atcggtcgag ccgacgatcg
     4861 ccgcgagcag catgcccagc cgccagtcga catcgaggag ccaggtggcg aacacgccga
     4921 gcagggccgc cgtaccgatc acgccccagc tggccagcac cgcggccggc ctcagcgcca
     4981 cccggaagct gctcacccgc gtgcgcaggc cgccgtcgag caggatcacc gagagcgccg
     5041 cctgcgcgat cagcgaggcg atgaagaagt cctcgaagac gatgccgccc ggcccgtcct
     5101 cgccggcgag catgccgacg ccgagaaaga gcagcagcag cggcaggccg agccgggcgg
     5161 agatggtgct ggcgagcacg ctgatgaaca gcagcaggcc ggtgagcagg aagagcgcgt
     5221 tgattccggt catgcggcgg cggggtccag ccggttggaa ggggcttttg gcaaaacggg
     5281 gcgatcgcac acggcacacc cccacagcgg agccgcccgg gcggaagaaa gttcctgcgc
     5341 acgcgggcgc ggagatcgcc cgtcggcacg cgccggggtc agcgcggaac ggcgccgggt
     5401 tcgagtacca ccacgtgctg gacgagttcg gcgagcgaat gcgcgcccat cttctccatc
     5461 acgcgcgcgc gatgcacctc gacggtcttg atgctgatgc cgagcacgtc ggcgatctgc
     5521 ttgttgagct tgccgacgat gatcagctcg agcacctcgc gctcgcgctg ggtaagcagc
     5581 ccgagccggc gcgcggtgtc ggcctcctgc aggcggcggt cgcgggtctc gcgctcgagg
     5641 cgcaggcact gctcgaccac gcgcagcatc tcgcgctcgc cgaagggttt ctcgacgaaa
     5701 tccaccgcgc cctgcttgag cgcggacacc gccatcggca cgtcgccgtg gccggtgatg
     5761 aacaccaccg gcagcccgca gcgccgccgc ccgagctcct cgaacacctc cagcccgctc
     5821 atgcccggca tgcgcacgtc gagcagcacg cagccggtca tctccggcgt gcaggcggca
     5881 aggaaggcct cgcccgactc gtgcaccgcg accgcgtggc cgttggcctc cagcatccag
     5941 gcgagcgagt cgcgcagggc ttcgtcgtcg tcgacgacat gtacgatctg gtcaggcgct
     6001 gcgggcactg cgctcaattg cggactccgt cggcagggta aacgtgaata tggtaccgcc
     6061 ttcggggttg gcgtcgacca gcaatctgcc gttgtggaac tcgacgatcg agcggcagat
     6121 gttgagcccc atgcccatgc cctcggcctt ggtggtgtag aagggcgtga acagctgcgc
     6181 cctgccctcc tcgctgatgc cgtggccgcg gtcgatcacc gcgacctcga ccgcgtccgc
     6241 gccgacggtg cgcacccgca ccgtcagcac gcgcgcctcg ggcaaggcgt cggccatggc
     6301 gtcgaggccg ttgcggatca ggttgagcag cacctgctcg atcatgatgc ggtcggcgta
     6361 caccggcggc agcccgggct cgagctccgt gtggatgcgg atgcgggtgc ggtgggcgtc
     6421 gatgtcggcg aagccgatcg cgtcctccag cacctcgacg aggtcgaccg cgcagcgctg
     6481 cggctcgctc ttcttcacga actcgcgcac gcgacggatg atcttgccgg cgcgctcggc
     6541 ctggaagctc gccttctgca tcgcggcgag cacgtcctcg gtccttccct tgcccgactg
     6601 caggcgcgtc accgagccgg cgcagtagtt ggcgatcgcc gccagcggct ggttgagctc
     6661 gtgcgccagc gtcgaggcca tctcgcccat ggtgatcagg cgcgaggtgc gctgcaggcg
     6721 ctcctcctgc tcgcgcgcca ccgcggcggt ctgcttgcgg tcggtgatgt cggtggcgat
     6781 ccccatgcgc accacgcgcc cgtccaccca gcgcgtggcc tgctcgcgca cgtggtacca
     6841 gcgccccgac tgcgggtgca gcagctcgcc atcgaagagc tcgcgtggca cgtcggcggg
     6901 cgacaggttg cgtggatcga cgcggtagtc gccgcgctcg ggctgcggca ccgccactcc
     6961 gcgcaccgtg cgcccgaccg cgtcgaaacc gtggatgcgc ttgaaggcaa ggttggcgaa
     7021 gaggatctcg tcggtgcgcg catcggcgac gaacaccgcg gcgtccaggc cgtcgagcac
     7081 cgcctcgaaa cgctcgtgcg ccgactcgag cgcggcgcgc acgcgcttgg gctcggtgat
     7141 gtcggtgatc gaggccatcc agccggtctg ccggccggtg agatcgatca acggcgacag
     7201 gtagaagcgc gcatcgaagc gctcaccgtt cttgcgccgg atgcgcatct cgaagccgct
     7261 gcgcggcgca cgccccgaca gcgtcatcga caggttgcgc tcgcactcgg cgagctgctc
     7321 ctgcggccag tacggaaacg gcggcccggc accgacgagc tcgtcctcgt cgaagccgat
     7381 catgcggcag aaggcggagt tgacgtagat gatccgcccc gacaggtcga tcgcgcgcag
     7441 gccggtgacg accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccgaca
     7561 ggtcgatcgc gcgcaggccg gtgacgaccg agtcctccat cgccttgcgg aaggcggact
     7621 cggcgcgcag cgcgtcctcg gtggccttgc gtccggtgac gtcgtgcgcc acggcgtaga
     7681 ccaggcgctc ctcgcgtacc gggttgatgc tccacatcag ccatttgtag ctgccgtcgg
     7741 cacagcggca gcgcgtctcg aagcgcaccg gttcgccacc ggcaaggcgg cgcaacatcg
     7801 ccagcgtctc ggaaacgtcc tcgggatgca cgaagtcgat cagcgagcac ccgggcaggc
     7861 tccgggggtc gtgcccgagc acgcgctcga aggccgggtt gcagcgcccg aaacgaccat
     7921 cgagatcgat gacgcacaag aggtcgagcg aaaggtcgaa gagctggtcg cgctccttct
     7981 ccacccgcac ccggcgctgc acgtggcggc gcagcgtcca caggctccac agcaccagca
     8041 gcgacagccc cacgatgagc accgccggca ggtagcgcag caccgcccca ccgggccgga
     8101 aggccatcgc ctgcagcgcg aggccgttgc ccggcggatc gagcgcgagg gtaagcgaga
     8161 gcgcctcctg cgcaggcggc agggccgagc tgagggtcag cacgccgcca tcgcggtcga
     8221 gcaccgcaag ccggtatttt tcggcaaagc gcgccggcac cagccggctc agcatgcgct
     8281 cgatcgaata cacggcgacg atcgcaccga gcgaatcgcc gcgccgcagc acgggcgcgt
     8341 acacctcgat caccgcgctg ccgcgcgcgt cgatgtaggg ccggccgtag gtcaggcggg
     8401 cggacagggc gctgttctgg aaggcctcca cctggtcatc cacgaggatc tcgccggcga
     8461 gcaggtcacg ggtgtcgaag ggcgccgacc aggcgatcac atgctcgcgg tccacccagg
     8521 cgaggttggc gagcgcgggg ttgatcgcca gatgctcgtt ggcgcgcagc tggaagtcgt
     8581 cgtggtcgag cgtgccagcc gacaggtcgc gtgcgagctg gacgagaaac tcctcggtgg
     8641 tgagcatggc gcgccggatc gactgctcgg tccactgcag gtcgcgcgcg agcgcgtcgc
     8701 gctcgaccgc ggtctcgcgc gcctgcagca gccagacgac cgcgagcatc gacagcgcga
     8761 gcacggtcac cgcgagatag ggcatggccc agtaccagcg cgacagggtc gcccgggcgg
     8821 cggcggaggc ttgcatggag gatggccgga cgagcggcgc ggggaggcgg atcgggagca
     8881 agattgttgt cgagcacgac cgcccgcgtc atccggtgta accctgatcg ggggacacat
     8941 acccggtgtg gatgcggcag cgcagcagac ggcgatgggg tttccgccaa tttgtgcgct
     9001 tgttcacgct gcctacagtg caaggatgaa acccttcctc gcgctgctcg ggcgcgcctt
     9061 cgtgacgctt ctgctcctgg cgccgcagtt gtcggcacag gccgtcccgg cagaggccgt
     9121 gggcgcgggc gcacgcccga gcggcggcga gcgggcaccg atccgggtgg gcgtgtcggg
     9181 gcccttctcc ggtccgtcgg cgccgatggg gctgtcgatg cgcgagggca tccgcatcgc
     9241 cgccgaggag ctcaacgccg gcggcggcct gctcggtcgg cgcatcgagc tcgtcgagcg
     9301 cgacgacgag gcgagcaacg agcttggcgc gcagatcgtg cgcgacttca tccatcgcga
     9361 acgcgtcacc gccggactgg gcatcgtcaa taccggtgtc gcgctcgcca gccagcgcca
     9421 ttaccagatg gcgcgcatcc cggtgatcac ctcggtcgcc accggctcgc tgatcaccaa
     9481 gcagttccag ccccccgatt tcaccgagaa ctacgtgttc cgcgtctccg ccagcgacac
     9541 cttgcaggcc gcggtgatcg tcgaggaggc ggtcggccgg cgcggcctga cccggctcgc
     9601 gatcctgcac gacgccacca actacggggt gctgggaagc caggatctca tcgccgccct
     9661 gggcacacgc gggcggaccg cggtggtggt cgagcgcttc cagttgcgcg agaccgacat
     9721 gcgaccgcag ctcgaacgcg cacgcgccgc cggtgcgcaa gccgtgctca cctatggcat
     9781 cggccccgag ctcgcccaca tcgccaactc gatggcccgc ctcggatggc aggtgccgat
     9841 catcggcagc tggacgctgg ccatgtcgag cttcatcgaa ctcgccggcc gcaacgccga
     9901 gggcgcacgc atgccacaga ccttcatcgc cgaggcacgc agcccggcgc aggcggcctt
     9961 cctcgcggcg tgggagcgcg ccaccggcag cgtgcgtatc ccggtgccac cggcggccgc
    10021 acagggctac gactcgatgc agctgctcgc cgcggcgatc cgccaggccg gcagcctcga
    10081 cggtccgcgc atccgcgagg cgctcgaaaa cctggatgcg gaggtcgatg gcgtaatcat
    10141 gcgctatcgc cgccccttct cccgcgacaa ccacgaaacc ctgcgcagcg cacgccagat
    10201 ccacctcggc gagatccgcg acggcgccgt ggtgttcgcc cacgaggcga cgccgggcag
    10261 gccgggacca tgagcgcgag cgatcaggga gccgatttcg tgttcaccag gtgaaaacat
    10321 attgcacatt gcgaaaagac gttttatatt ttgaaacgct gaccgtgcgg cagccggcat
    10381 ggtcgtgccc gtaaccaaca acccgcctgt cccgccagac aggaatcgcc aactgaacgt
    10441 catgaggaga caaaccatga ccggaatcag caccgttctc ccgcaaaccg acacggatgc
    10501 gcaggaaacg aaggaatggc tggacgcgct cgccgccgtc gtcgaacacg aaggccccga
    10561 gcgcgcccac tacctgatcg agaccctgat cgccacggcg cgccaggagg gggtgaacat
    10621 cccctactcg gccacgaccg agtacatcaa cacgattccg gccgagcgcc agccgcccta
    10681 cccgggcgat ccggatctgg agatcaagat ccactcctac atccgctgga acgcgatggc
    10741 gatggtggtg cgggcgaaca aggacaccaa cgtgggcggt cacatcgcct ccttcgcctc
    10801 ggcggcggcg ctctacgacg tgggcttctc gcacttctgg cacgcgccct ccgaggcgca
    10861 cgacggcgac ctgatctact tccagggcca ctcggtgccg ggcgtgtatg cgcgcgcgtt
    10921 catgctgggc cgtctgaccg aagagcagat ggacagcttc cgccaggaag tgggcggcaa
    10981 gggcatctcg tcctacccgc acccctggct gatgcc
//
LOCUS       DAOQNN010000061        11013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2756_length_11013_cov_9.938401, whole genome shotgun sequence.
ACCESSION   DAOQNN010000061 DAOQNN010000000
VERSION     DAOQNN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11013
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2756_length_11013_cov_9.938401"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PKV97_05470"
     CDS             complement(<1..559)
                     /locus_tag="PKV97_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNB05362.1"
                     /translation="MNILQINGSARSQAGNSTRLASEVVARLVAAEPAARVVVRDLAR
                     DPAPAIDEAALGALFTPAEQRSPEQVVRVAQSDALVAEVQAADVLVLGVPMYNFGIST
                     QLKNWIDAIARAGVTFRYTENGPEGLLKGKKAYVTFARGGRYRGTAADTQTPYLKTLL
                     GFLGITDVHFIYAEGLNMGEEATKQG"
     gene            655..1563
                     /locus_tag="PKV97_05475"
     CDS             655..1563
                     /locus_tag="PKV97_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB05363.1"
                     /translation="MEQIDPNDLLVFARVVAEGSLTAAADKLGLPKSTVSRRLSRLEE
                     QLGERLLLRTTRRLNITEFGAALLDHARQLESEVEAVAALAESRQARPSGRLRVSMPS
                     DFANLLLTDMLAAFAALHPAVTLELDLSPRRVDLLGENFDLAIRLGDLPDDALLAARR
                     LAVFPWGLYAAPAYLAEHGEPQAPDDLHRHRALRLLARTGDAIRWTLLCGDQRWEGVP
                     PGSITANSPELLIRFACAAAGITTVPEYFAAPHVQRGELRRVLPDWRPPSIAAWAVFP
                     GRRLMPAKTRAFLDMLQAALGGDQRG"
     gene            1742..2248
                     /locus_tag="PKV97_05480"
     CDS             1742..2248
                     /locus_tag="PKV97_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HNB05364.1"
                     /translation="MTLLHRILNLPARVLYLALFLVATGLLGFGLYLQHGLGLEPCPM
                     CIMQRYAFATAALIALIAGLHAPGRGGERIYAVLIGAAALTGGMIAARQSWMQLNPPL
                     IPECGPGLEFMLESFPLAQALPMIFRGAGDCTVIEWTFLGLSIANWSLVSFSATVLFA
                     GWILLRRS"
     gene            complement(2253..2474)
                     /locus_tag="PKV97_05485"
     CDS             complement(2253..2474)
                     /locus_tag="PKV97_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNB05365.1"
                     /translation="MTTNEYVRAFAGAFVLASLAFGVEASPLFVSKHFLWVTGFVGLN
                     LFQSAFTGICPLVNILHKLGVKDGPASCS"
     gene            complement(2471..5761)
                     /locus_tag="PKV97_05490"
     CDS             complement(2471..5761)
                     /locus_tag="PKV97_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB05366.1"
                     /translation="MEKSLGISGRIAAGFQRNAITPLLALVLLLAGIFATLITPKEEE
                     PQIDVTMANVLVPFPGASVRDVEALVARPAEQVLSRMAGVEHVYTVSRPGMAVITVQF
                     EVGVPNQTALVRLYDTVDSHADWLSPQLGVGQPLIKPKGIDDVPIVSFTLWTADPERA
                     AFSLQQVSRAIETELKRIPGTRDVATIGGPGHVIRVDMDIERMNAHGVTAQDLRGALQ
                     LANASQPAGSLVAGNKEVLVQTGTYLESAEDVRNLVVGVSERRPVFLRDVAEVVDGPD
                     QPAQYVWFGTGAAAAERGIDQHGLFPAVTLALSKKPGANAADVARAAMQRLENLRGSL
                     IPEGVELTVTRDYGKTANDKAKQLIGKLAFATAAVVALVFFALGRREAIIVGIAVSLT
                     LAATLFASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRWHTLEPDTALWKLIP
                     RAVDEVGGPTILATFTVIAALLPMAFVTGLMGPYMSPIPINASMGMFISLVVAFVVTP
                     WLAQKMLKNVDAHQHGGEDRMTKRLDGLFRRVMTPMFDPRRGARNRLMLWLAVFALIG
                     ASVALPVVQLVVMKMLPFDNKSEFQVVLDMPAGTPVEDTARVLNEIGAHLATVPEVTD
                     WQSYAGSAAPINFNGLVRQYYLRSAPEQGDIQVNLVDKTARERQSHEIAVSVRDAVTA
                     IALRNGGNAKVVEVPPGPPVLSPIVAEIYGPDYDGQVAVAKRVRAAFEDTPDIVGVDD
                     TVDEDAPKLVLRVDQAKAARMGVAQADIVEVVRLGLSGENVTPVHGGDNKYEIPVRIQ
                     LPAVRQSDLADLLAMQVRARGGDNAGQRVKISELVQVAETRREQILYRKDLLPVVYVT
                     GDMGGKLDSPLYGMFDIRSRVNGMALEGTRGLAGTLGEWFIRQPVDPYAGYQLKWDGE
                     WQITYETFRDMGAAYAVGLVLIYLLVVAQFRSYLVPLIIMAPIPLTIVGVMPGHALLG
                     AQFTATSMIGMIALAGIIVRNSILLVDFVNQQVRGVDGKPGMEFGEALIRAAAVRAKP
                     IGLTALAAMIGAVFILDDPIFNGLAISLIFGIFVSTVLTLVVIPVLYYAAMRGRIASF
                     ENPEENPA"
     gene            complement(5776..6852)
                     /locus_tag="PKV97_05495"
     CDS             complement(5776..6852)
                     /locus_tag="PKV97_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB05367.1"
                     /translation="MKPIMLSVPARLLAVLVLSLLTVTSGGVRAELPTVRVEARAVVP
                     AIVAEATIEAVRQSTLAAQMPGRILELSVDAGDRVRRGAVLARIDPAEVAAAVAAADA
                     GIAQAESALANARAEYQRARSLVERKFLSQSALDNARAQFQAAEAQVRAARAQREQAA
                     TVKGYTSVVSPLDGLVAARHIEPGEMAQPGRNLLTVYDPAQMRAVADLAPQRLAELGA
                     GPLRARVELPASRRIVEAAAVTVLPAADARTHTVRVRVDLPAGIEDVLPGSFARVHFQ
                     GTAAAASTAGPTVIPAAAVLRRGELTAVYAADGQGGFTLRQIRLGRALPGGEWVEVLS
                     GLGGDETLALDPVQAGIQAHAAAR"
     gene            complement(7004..7390)
                     /locus_tag="PKV97_05500"
     CDS             complement(7004..7390)
                     /locus_tag="PKV97_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HNB05368.1"
                     /translation="MNAANPSASAIVLFGHGARDPEWAGPMRRIRDAALARPGAPRVE
                     LAFLEFMSPTLPEAIAALAAEGAHKVAVVPIFLAQGGHLKRDLPVLLEEARAAHPGCE
                     ITLATAAGEADPVVAAMADYALGCLG"
     gene            complement(7387..8211)
                     /locus_tag="PKV97_05505"
     CDS             complement(7387..8211)
                     /locus_tag="PKV97_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNB05369.1"
                     /translation="MTSATTDRARRFGGIDRLYGEGAAARLAGAHACVIGIGGVGSWA
                     AEALARSGVGRLTLIDLDHVAESNINRQAHALEDTLGMAKVSAMATRIAGIDPACRVD
                     TIEDFLTPENAAELLHGFDLVIDAIDNVRAKVAIAVTCRERRIPLVMAGGAGGKRDPA
                     RIGMDDLARTLQDPLLAKVRARLRKEHGFPRDPKKKFGIEAVFSTEPLHFPAVDACDA
                     AHPGAASAPQGLACAGYGSSMAVTASVGLLCAARALEHLLRQADAPARDATPEPSA"
     gene            complement(8219..9181)
                     /locus_tag="PKV97_05510"
     CDS             complement(8219..9181)
                     /locus_tag="PKV97_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB05370.1"
                     /translation="MKDLIHYGDGVHAIDSGYGRPQLAAIHVIVQDGRAAIVDSASNA
                     SVPRILGALAALDIAPEAVDWVLLTHIHLDHAGGAGSLMCALPQARLLVHPRGLRHMI
                     DPGKLWEATCAVYGPERAFDLYGQLVPVPAERIVPATDGLEIHVGSRMLRVFDTPGHA
                     RHHICIWDSVARAFFTGDTFGLSYRELDVDGRAFVVPTTTPTQFDPEGMHASIDRMLA
                     MRPQAMYLTHYSRVGEVDRLGADLHRLIDTMLAVAEAARGDGVARHVEIVAGLEQIFR
                     EEAARQNWALGEEETLELLRMDLELNAQGLGVWLDSRQTGATGA"
     gene            complement(9298..9801)
                     /gene="ssb"
                     /locus_tag="PKV97_05515"
     CDS             complement(9298..9801)
                     /gene="ssb"
                     /locus_tag="PKV97_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNB05371.1"
                     /translation="MASVNKVILIGNLGADPESRFAPSGDAICNIRLATTETWRDKNT
                     GERREATEWHRVAFFGKLAEIAGQYLRKGSQVYIEGSLRTRKWQDQSGQDRYTTEIRA
                     DEMKMLGRREGGDAPMRGGEGGGWDAGAQSAPARQPAAPAQRAQPQSAPPQQSGGFGD
                     FDDDIPF"
     gene            complement(9871..>11013)
                     /locus_tag="PKV97_05520"
     CDS             complement(9871..>11013)
                     /locus_tag="PKV97_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB05372.1"
                     /translation="AIFALRMLGLFLILPVFAVHAATIPGGGDLTLVGLAIGAYGLTQ
                     ACLQIAYGAASDRFGRKPVIVFGLVLFVLGSVVAALADGIHMIIVGRVLQGAGAISAA
                     VTALAADLTRDQHRTKVMAMIGSSIGLVFALSMVAAPLLYAAVGMDGIFWLTAVLAAG
                     AIGVLLWAVPVAPPVPRATGRLIDVLRDGQLMRLNFGVFALHLIQTTMWVMVPSALVS
                     GGGLPVPEHWKVYLPAVLLSFVVMVPAVIVAERHDKLKPVFNAAIVLLAVVQIGLWLF
                     GDGLIPLALLLTLFFISFNVLEATQPSWISRIAPPGSKGTALGVYNTLQSIGLFLGGV
                     LGGWLGQTFGPAAVSLSCAALALLWLALASTMNPPPLRAAPAGAPR"
BASE COUNT         1680 a         3778 c         3963 g         1592 t
ORIGIN      
        1 agccctgctt cgtggcctct tcacccatgt tcaggccttc ggcgtagatg aaatgcacgt
       61 cggtgatgcc gagaaaaccg agcagggtct tcaggtaagg cgtctgggtg tcggccgcgg
      121 tgccgcggta gcgcccaccg cgcgcaaagg tgacataagc cttcttgccc ttgagcaggc
      181 cctcgggacc gttctcggtg tagcggaagg tgacgccggc gcgagcgatc gcgtcgatcc
      241 agttcttgag ctgggtggag atgccgaagt tgtacatcgg cacgcccagc accagcacat
      301 cggccgcctg tacctcggcg accagcgcgt cgctctgcgc cacgcgcacc acctgctcgg
      361 ggctgcgctg ctcggccggg gtgaacaggg cgccgagcgc ggcctcgtcg atcgccggcg
      421 cagggtcgcg ggcgaggtcg cgcaccacca cacgggcggc cggctcagcg gcgaccaggc
      481 gggcgacaac ttcgctggcg aggcgggtgg agttaccggc ttgggaacgg gcgctaccgt
      541 tgatctgcag gatgttcatg tgcttgtcct ctggcttggg gatgggatgg attgcagtgt
      601 attggatgcc ggggattgca ttaagcccgt tcgacgcaca tgattgttcc ctatatggaa
      661 caaatcgacc ccaacgacct gctcgtcttc gcccgcgtgg tggccgaggg cagcctgacc
      721 gccgcggccg acaagctcgg cctgcccaag tccaccgtct cgcgtcggct gtcgcggctg
      781 gaggagcagc tcggcgagcg cctgctgctg cgcaccaccc gtcgtctcaa catcaccgag
      841 ttcggcgccg ccctgctcga ccacgcacgc cagctcgaga gcgaggtcga ggcggtcgca
      901 gcgctcgccg agagccgcca ggcgcgcccg agcgggcggc tgcgggtgtc gatgccgagc
      961 gacttcgcca acctgctgct caccgacatg ctcgccgcct tcgccgcgct gcatcccgcg
     1021 gtgacgctgg agctcgacct gtcgccacgc cgggtggacc tgctcggcga gaacttcgac
     1081 ctcgcgatcc gcctcggcga cctgccggac gacgcgctgc tcgccgcgcg ccggctggcg
     1141 gtgtttccgt gggggctgta tgccgcaccc gcctatctcg ccgagcacgg cgagccgcag
     1201 gcgccggacg acctccaccg ccatcgcgcg ctgcgcctgc tcgcgcgcac cggcgacgcg
     1261 atccgctgga ccctgctgtg cggcgatcag cgctgggaag gcgtgccgcc gggctcgatc
     1321 acggcgaact cccccgagct gctgatccgc ttcgcctgcg cggctgcggg catcaccacc
     1381 gtgcccgagt atttcgccgc gccccacgtg cagcgcggcg agctgcgccg ggtgctgccc
     1441 gactggcgtc cgccctcgat cgccgcctgg gcggtgttcc cgggccggcg gctgatgccg
     1501 gcgaagacgc gggctttcct ggacatgctg caggcggcgc tgggcggcga tcagcgcggg
     1561 tgagagggac ccgggcttgc acagcctcgc gaagcgggct tgcctcgctc ccacggaatg
     1621 cacggcggcc cccgacctcc gccgcccacg gggaacaccg caagcatgca cgggtccgag
     1681 agcgcggacg ccgctcgcct gctcgcctac aatggcgccc tttcccggaa gatcgaatac
     1741 gatgaccctg ctacaccgca tcctgaacct gcccgcgcgg gtgctctacc tcgccctgtt
     1801 cctggtcgcc accgggctgc tcggcttcgg cctctacctg cagcacggcc tgggcctgga
     1861 gccctgcccg atgtgcatca tgcagcgcta cgccttcgcg accgccgcgc tgatcgcgct
     1921 gatcgccggc ctgcatgcac ctggacgcgg cggtgagcgc atctacgcgg tgctgatcgg
     1981 agcggccgcg ctcaccggcg gcatgatcgc cgcgcgtcag agctggatgc agctcaaccc
     2041 gccgctgatc ccggagtgcg gcccggggct ggagttcatg ctcgagagct tcccgctcgc
     2101 gcaggccctg ccgatgatct tccgcggcgc gggagactgc accgtgatcg aatggacttt
     2161 cctcggtctg tcgatcgcga actggtcgct ggtgagcttc agcgcgaccg tgctgttcgc
     2221 cggctggata ctgctgcggc ggagctgaac actcagctgc agctcgcggg tccgtccttc
     2281 acgcccagtt tgtgcaggat gttgaccagc ggacagatgc cggtgaaggc gctctggaag
     2341 aggttgaggc cgacgaagcc ggtcacccac aggaagtgct tgctgacgaa gagcggcgag
     2401 gcctcgacgc cgaaggccag cgaggccagg acgaaggcgc cggcgaaggc gcgcacgtat
     2461 tcattggtgg tcatgcggga ttctcctcgg ggttctcgaa gcttgcgatg cggccgcgca
     2521 tggccgcgta gtagagcacc gggatcacca ccagggtgag cacggtggac acgaagatgc
     2581 cgaagatcag gctgatcgcc agcccgttga agatcgggtc gtcgaggatg aacaccgcgc
     2641 cgatcatcgc cgccagcgcg gtgagcccga tcggcttggc gcgcaccgcg gcggcgcgga
     2701 tcagcgcctc gccgaattcc atccccggct tgccatccac ccctcgcacc tgctgattga
     2761 cgaaatccac cagcaggatc gagttgcgca cgatgatgcc ggcgagcgcg atcatgccga
     2821 tcatcgaggt ggcggtgaat tgcgcgccga gcagcgcatg accgggcatc acgccgacga
     2881 tggtgagcgg gatcggcgcc atgatgatca gcggcaccag gtagctgcgg aactgcgcca
     2941 ccaccagcag gtagatcagc accaggccca cggcgtaggc cgcgcccatg tcgcggaagg
     3001 tctcgtaggt gatctgccac tcgccgtccc acttcaactg atagccggcg taggggtcga
     3061 cgggctggcg aatgaaccac tcgccgagcg tgccggcaag cccccgggta ccttcgagcg
     3121 ccatgccatt cacgcgcgag cggatgtcga acatgccgta cagcggcgaa tccagcttgc
     3181 cacccatgtc gccggtcacg tacaccaccg gcagcaggtc cttgcggtag aggatctgct
     3241 cgcggcgcgt ctcggcgacc tgcaccagct cggagatctt cacccgctgg ccggcgttat
     3301 cgccgccgcg cgcgcgcacc tgcatggcga gcaggtcggc gaggtcggac tggcgcaccg
     3361 ccggcagctg gatgcgcacc gggatctcgt acttgttgtc gccgccatgc accggggtga
     3421 cgttctcgcc ggagaggccg aggcgtacga cctcgacgat gtcggcctgc gccacgccca
     3481 tgcgcgcggc cttggcctgg tcaacgcgca gcacgagctt gggcgcgtcc tcgtccaccg
     3541 tgtcgtccac gccgacgatg tcgggggtgt cctcgaaggc ggcacgaacc cgcttcgcga
     3601 ccgccacctg gccgtcgtaa tccggaccgt agatctcggc cacgatcggc gacagcacgg
     3661 gcggcccggg cggcacctcc accaccttgg cgttgccgcc gttgcggagg gcgatcgcgg
     3721 tgacggcgtc gcgcaccgac accgcgatct cgtggctctg gcgctcgcgc gcggtcttgt
     3781 cgaccaggtt cacctggatg tcgccctgtt cgggggcgct cctcaggtag tactggcgca
     3841 caaggccgtt gaagttgatc ggcgccgcgc tgccggcgta ggactgccag tcggtgacct
     3901 cgggcacggt ggccagatgt gcgccgatct cgttcaacac gcgcgccgtg tcctcgaccg
     3961 gcgtgccggc cggcatgtcg agcacgacct ggaactcgct cttgttgtcg aagggcagca
     4021 tcttcatgac cacgagctgc accaccggca gcgccaccga ggcgccgatg agcgcgaaca
     4081 cggccagcca cagcatcagg cggttgcgag cgccgcggcg ggggtcgaac atcggcgtca
     4141 tcacgcgacg gaagaggccg tcgagccgct tcgtcatccg gtcttcgcca ccgtgctgat
     4201 gggcgtcgac gttcttcagc atcttctgcg ccagccaggg cgtgaccacg aaggccacca
     4261 ccagcgagat gaacatgccc atcgaggcgt tgatcgggat ggggctcatg tagggaccca
     4321 tcagcccggt cacgaaggcc atcggcagca gcgccgcaat cacggtgaag gtggcgagga
     4381 tggtagggcc gccgacctcg tcgaccgccc tggggatcag tttccacagc gccgtgtcgg
     4441 gctcgagggt gtgccagcgg tggatgttct ccaccacgac gatggcgtcg tcgaccagga
     4501 tgccgatcga gaagatgagc gcgaacagcg acacccggtt gagcgtgaag ccccaggccc
     4561 aggaggcgaa cagcgtcgcc gcgagcgtga gcgacaccgc gatgccgacg atgatcgcct
     4621 cgcgccggcc gagcgcgaag aacaccagcg ccaccaccgc ggcggtggcg aaggcgagct
     4681 tgccgatgag ctgcttggcc ttgtcgttgg cggtcttgcc gtaatcgcgc gtgacggtga
     4741 gctccacgcc ctccgggatg aggctgccac gcaggttctc caggcgctgc atcgccgcgc
     4801 gggcgacatc ggctgcgttg gcgccgggct tcttcgacag cgccagggtg accgccggga
     4861 agaggccgtg ctgatcgatg ccgcgctccg cggcggcggc gccggtgccg aaccagacgt
     4921 actgcgcggg ctggtcgggg ccgtcgacga cctcggcgac gtcgcgcagg aagaccggtc
     4981 ggcgctcgga gacgcccacg accaggttgc gcacgtcctc ggcggactcg aggtaggtgc
     5041 cggtctgcac cagcacttcc ttgttgcccg cgaccaggct gcccgccggc tgcgaggcgt
     5101 tggcgagctg cagcgcgccg cgcaggtcct gcgcggtgac gccgtgcgcg ttcatgcgct
     5161 cgatgtccat gtccacccgg atcacgtggc ccgggccgcc gatggtggcg acgtcgcggg
     5221 tgccggggat gcgcttgagc tcggtctcga tcgcacgcga gacctgctgc agcgagaagg
     5281 cggcacgttc gggatcggcg gtccacagcg tgaagctgac gatgggcacg tcgtcgatgc
     5341 ccttgggctt gatcagcggc tggccgaccc cgagctgcgg cgacagccag tcggcatgcg
     5401 agtccaccgt gtcgtagagc cgcaccagtg ccgtctggtt gggcacgccg acctcgaact
     5461 gcacggtgat caccgccatg ccggggcgcg acacggtgta gacatgctcg acgcccgcca
     5521 tgcgcgacaa cacctgctcg gccggccgtg ccaccaaagc ctcgacgtcg cgcaccgagg
     5581 cccccgggaa gggcaccagc acgttggcca tggtgacgtc gatctgcggc tcttcttcct
     5641 tgggcgtgat cagcgtggcg aagatgccgg ccagcagcag caccagcgcc agcagcggcg
     5701 tgatcgcgtt gcgctggaaa ccggccgcga tgcggccgga gatgccgagg gacttttcca
     5761 tggtcgcctt ccggctcagc gcgcggccgc gtgggcctgg atgccggcct gcaccgggtc
     5821 gagggcgagg gtctcgtccc caccgagacc ggagagcacc tcgacccact cgccgccggg
     5881 gagcgcgcgg ccgagacgga tctggcgcag cgtgaagccg ccctgcccgt cggcggcgta
     5941 gacggcggtg agctcgccgc gacgcagcac ggcggcagcc gggatcacgg tcggcccggc
     6001 ggtgcttgca gcggcggccg tgccctggaa atgcacgcgc gcgaagctgc cggggagcac
     6061 atcctcgatg ccggccggca ggtccacgcg cacgcgcacg gtgtgggtgc gcgcgtcggc
     6121 ggcgggcagc accgtgaccg cggccgcctc gacgatgcgc ctgctggcgg gcagttcgac
     6181 cctggcgcgc agcgggccgg cacccagctc ggcgagacgc tgcggggcga gatcggccac
     6241 ggcgcgcatc tgcgcggggt catacacggt gagcaggttg cgcccgggct gtgccatctc
     6301 gccgggctcg atgtggcgcg cggcgacgag gccgtcgagc ggcgacacca cgctggtgta
     6361 gcccttcacg gtggcggcct gctcgcgctg ggcgcgcgcg gcgcgcacct gagcctcggc
     6421 ggcctggaac tgcgcgcgcg cgttgtcgag cgccgactgg ctgaggaact tgcgctcgac
     6481 caggctgcgc gcgcgctggt attcggcgcg cgcgttggcg agcgcgctct cggcctgggc
     6541 aatgccggca tcggccgcgg cgacggcggc ggcaacctcg gccgggtcga tgcgcgccag
     6601 caccgcgccc ctgcgcacgc gatcgccggc gtccacggag agctcgagga tgcgaccggg
     6661 catctgcgcg gccagggtgg actggcgcac ggcctcgatg gtggcctcgg cgacgatcgc
     6721 ggggacgacc gcgcgtgcct cgacgcggac ggtgggaagc tccgcacgca ccccgccgga
     6781 agtgacggtc aacaggctca ggacaagaac agccaggaga cgggcaggaa cggagagcat
     6841 gatcggcttc ataagaataa gtatattctt atattgaagg ccgtcaagga atacccctcg
     6901 agaaccgcga gagacagcac tactgcaggc aggagcgacg cgagtcacga tcccgccgac
     6961 accgcggcag cgccgcggat tgcgcgcgcc gtcgggtcgg gcctcagccc aggcagccga
     7021 gcgcgtagtc ggccatcgcg gcgaccaccg ggtcggcctc gcccgcggcg gtcgcgaggg
     7081 tgatctcgca gccggggtgg gcggcgcgcg cctcctccag caacaccggc agatcgcgct
     7141 tgaggtggcc gccctgggca aggaagatcg gcaccacggc cactttgtgc gcgccctcgg
     7201 cggcgagcgc ggcgatcgcc tcgggcaggg tcggggacat gaactcgagg aaggcgagtt
     7261 cgacgcgcgg cgcccccgga cgggcgagcg ccgcatcgcg gatgcgccgc atcggcccgg
     7321 cccactcggg gtcgcgggcg ccgtggccga agaggacgat ggcggaagcg gacggattgg
     7381 ctgcgttcat gcggaaggct ccggggtagc gtcgcgcgca ggcgcatcgg cctgacgcag
     7441 caggtgttcg agtgcacgcg ccgcacacag caggccgacg ctggcggtca ccgccatgct
     7501 cgagccgtat ccggcgcacg ccagcccctg aggggcggac gcggcgccgg ggtgggccgc
     7561 gtcgcaggca tcgaccgcgg gaaagtgcaa gggttcggtg gagaacaccg cctcgatgcc
     7621 gaatttcttc ttcgggtcgc gggggaagcc gtgctccttc cgcaggcgag cgcgcacctt
     7681 ggcgagaagc ggatcctgca gcgtacgcgc gaggtcgtcc atgccgatgc gcgcagggtc
     7741 gcgcttgccg ccggccccac ccgccatcac caaggggatg cggcgctccc tgcaggtcac
     7801 cgcgatcgcg accttggcgc gcacgttgtc gatcgcatcg atcaccagat cgaagccgtg
     7861 cagcaactcg gcggcgttct ccggggtgag gaaatcctcg atcgtgtcca cccggcaggc
     7921 cgggtcgata cctgcgatgc gcgtcgccat ggcgctcacc ttggccatgc cgagggtgtc
     7981 ctcgagcgca tgggcctggc ggttgatgtt ggattcggcg acatggtcga ggtcgatcag
     8041 ggtcagccgc cccaccccgc tgcgcgcgag cgcctcggcg gcccacgagc cgacgccgcc
     8101 gatgccgatc acgcaggcgt gcgcgcccgc gaggcgcgcg gccgcgccct cgccgtaaag
     8161 acggtcgatg ccgccgaacc ggcgcgcacg gtcggtcgtg gccgaagtca tggcaggctc
     8221 aggcaccggt ggcgccggtc tggcggctgt ccagccacac ccccaggccc tgggcgttga
     8281 gctcgaggtc catgcgcagc agctcgagcg tctcctcctc gccgagcgcc cagttctggc
     8341 gcgcggcctc ctcgcggaag atctgctcca gcccggcgac gatctcgacg tgacgggcga
     8401 cgccgtcgcc gcgcgcggcc tcggccaccg cgagcatggt gtcgatcagg cggtgcaggt
     8461 cggcaccgag gcgatcgacc tcaccgacgc ggctgtagtg cgtcaggtac atcgcctgcg
     8521 gccgcatcgc cagcatgcga tcgatcgagg cgtgcatgcc ctcggggtcg aactgcgtcg
     8581 gcgtggtggt cggcaccacg aaggcgcggc catccacgtc gagttcgcgg taagacaggc
     8641 cgaaggtgtc gccggtgaag aaggcacgcg cgaccgagtc ccagatgcag atgtggtggc
     8701 gggcgtggcc cggggtgtcg aacacccgca gcatgcggct gcctacatgg atttcgaggc
     8761 cgtcggtggc cggcacgatg cgctcggccg gcaccggcac gagctggcca tacaggtcga
     8821 aggcgcgctc cgggccatac accgcgcagg tggcctccca cagcttgccc gggtcgatca
     8881 tgtggcgcag accgcgcgga tgcaccagca gcctcgcctg cggcagcgcg cacatcaggc
     8941 tgccggcacc gccggcgtgg tcgaggtgga tgtgggtcag cagcacccag tccaccgcct
     9001 cgggggcgat gtcgagcgcg gcgagcgcac cgaggatgcg cggcaccgag gcgttgctcg
     9061 cgctatcgac gatggcggcg cgcccgtcct gcacgatcac gtggatggcg gcgagctgcg
     9121 gccggccgta gccggagtcg atcgcatgca cgccgtcgcc gtagtggatc aggtccttca
     9181 ttgttcctcc cgaatggccg gttcgctgcc gcgcctgtcg atgtgaaacg ggcgagcgta
     9241 cgcgtcgcgt cgcccgcccg gcagtcggcg gagcgcagga cgcgctccag gcatccctca
     9301 gaacggaatg tcgtcgtcga agtcgccgaa gccgcccgac tgctgcggcg gcgccgactg
     9361 cggctgcgcg cgctgcgcgg gggctgcggg ctggcgggcc ggcgcggatt gggcgccggc
     9421 atcccagcca ccgccctcgc cgccgcgcat cggcgcatcg ccgccttcgc ggcggccgag
     9481 catcttcatc tcgtcggcgc ggatctcggt ggtgtagcgg tcctggccgc tctggtcctg
     9541 ccacttgcgg gtgcgcaggc tgccctcgat gtacacctgg ctgcccttgc gcaggtactg
     9601 gccggcgatc tcggcgagct tgccgaagaa ggcgacgcgg tgccattcgg tggcctcgcg
     9661 gcgctcgcca gtgttcttgt cccgccaggt ctcggtggtg gccaggcgga tgttgcagat
     9721 cgcgtcgccg gagggggcga agcggctttc cggatcggcg ccgaggttgc cgatcaggat
     9781 cactttattg acggatgcca tccggatctc ctttgcatgc gtttgccggc cgcgtcgggc
     9841 gtgccggcgg tgagtatggt gaagccgggc tcagcgcggc gcgccggccg gagccgcgcg
     9901 cagcggcggg ggattcatcg tgctcgccag cgcgagccag agcagcgcga gcgccgcgca
     9961 cgacaggctc accgcggccg ggccgaaggt ctgccccagc cagccgccga gcacgccgcc
    10021 gaggaacagg ccgatcgact gcagggtgtt gtagacgccc agcgccgtgc ccttggagcc
    10081 gggcggcgcg atacgcgaga tccacgacgg ttgggtggcc tcgagcacgt tgaaggagat
    10141 aaagaagagc gtcagcaaca atgccaatgg tatcaatccg tcaccgaaaa gccacagccc
    10201 gatctgcacg acggcgagca ggacgatggc ggcattgaac accggcttga gcttgtcgtg
    10261 gcgctcggcg acgatcaccg ccggcaccat gaccacgaag gacagcagca ccgccggcag
    10321 gtagaccttc cagtgctcgg gcaccggcag gccgccgccg gagaccaggg ccgagggcac
    10381 catcacccac atcgtggtct ggatgaggtg gagcgcgaac acgccgaagt tgagccgcat
    10441 gagctggccg tcgcgcagca cgtcgatgag ccggccggtg gcgcgcggca ccggcggagc
    10501 gaccggcacc gcccacagca gaaccccgat cgcacccgcg gcaagcacgg cggtgagcca
    10561 gaagatgcca tccatgccga cggcggcgta gagcagcggt gccgccacca tcgagagcgc
    10621 gaacaccagg ccgatcgagg agccgatcat cgccatgacc ttggtgcggt gctggtcgcg
    10681 ggtgaggtcg gcggcgagcg cggtgaccgc ggcggagatc gcccccgcac cctgcagcac
    10741 gcggccgacg atgatcatgt ggatgccgtc ggcgagcgcc gcgaccacgc tgccgagcac
    10801 gaacagcacc aggccgaaga cgatcaccgg cttgcggccg aagcggtcgg aggcggcgcc
    10861 gtaggcgatc tgcaggcagg cctgggtgag gccgtaggcg ccgatcgcca ggcccaccaa
    10921 ggtgaggtcg ccgcccccgg ggatggtggc ggcgtgcacc gcgaacaccg gcaggatcag
    10981 gaacaggccg agcatgcgca gcgcgaagat cgc
//
LOCUS       DAOQNN010000062        10999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2760_length_10999_cov_10.731268, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000062 DAOQNN010000000
VERSION     DAOQNN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10999
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2760_length_10999_cov_10.731268"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..774)
                     /locus_tag="PKV97_05525"
     CDS             complement(52..774)
                     /locus_tag="PKV97_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNB05373.1"
                     /translation="MTARILIDAARELSAAVDALPFAPPVTHVYNPLDYAWEVHRRYL
                     ERYGDGRRRVVFVGMNPGPFGMAQIGVPFGEIASARDWLGLEGPVGQPLIVNPKRPVE
                     GFACARSEVSGQRLWGLFRARYGAPEAFFAEHFVANYCPLVFFEGGRNLTPDKLPVAE
                     QRPLLAACDAHLRILVGALQPEWVIGIGNWAEKRASEALAGLPGVKFGRVLHPSPASP
                     AANRGWAEAATRQLTELGVWRS"
     gene            complement(771..1100)
                     /locus_tag="PKV97_05530"
     CDS             complement(771..1100)
                     /locus_tag="PKV97_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNB05374.1"
                     /translation="MSTTALLLLLVLVGLLTYLYRYAMIGLFASRSLPRWLHALCLHI
                     APASFAALTASALFVAGGEVVFAFDTPRPWAALVAVLVAWRTRNVLATIGAGMAALYL
                     LKLLFPT"
     gene            complement(1097..1840)
                     /locus_tag="PKV97_05535"
     CDS             complement(1097..1840)
                     /locus_tag="PKV97_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNB05375.1"
                     /translation="MAHTGTSARREFLAGCRDEAPLLLGVAPFGMIYGIAALAAGVPA
                     WLAQLASAIVFAGAAQLVIVQMLAAGAGFIPIALTSGLLNLRHVLYSASMAEYVRHLP
                     RRWRLLLAYVLTDEAYAVAVLRYQQRHAAAADHAEHTNATADLRHWYFLGAGFTLWAG
                     WQLSTAAGLLFGTTIPPEWELDFAVPLTFIALLTLLLRERAGQAAALVAGLGALAFAA
                     LPYKLGLVAAIVLGLAAGAWTVRRLEQAR"
     gene            complement(1840..3369)
                     /locus_tag="PKV97_05540"
     CDS             complement(1840..3369)
                     /locus_tag="PKV97_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB05376.1"
                     /translation="MSAPPSPAFARAWRLSAILVLGFASGLPLALTGQAMQAWLTVDG
                     VDLATIGFFGLVGVPYTFKFLWAPLMDRFEPPWLGRRRGWLALTQLALAALLWWMASL
                     SPTATPGLFAIAAVAIAFLSASQDVVVDAYRTDLLPEAERGLGASVHVFAYRLAMILS
                     GGIALIWAGQWASWPRVYETMALIMAACAVVSLLALPRVSAALKPLDSDPRRELLGFA
                     AMLAGVAAGYWSARQALILLGLDPNDANRWIQLLFVMAEIALALPLAGWAARRAGFET
                     LNRSLSSYFAQQGAAAFLALIILYKLGDAFAGSLTTPFLIKGMGFSQEEVGIANKVIG
                     IWLTILGAFIGGLIMTRLALYRSLLLFGVLQLVSNFGFYLLAELGKGAWGAVMVPAFD
                     WGFVAIDTPAALDRLLLTVIAGENISGGMGTVAFVALLMGLCNQRFTATHYAMLSAFA
                     AVGRIYVSPLSGVLSQSIGWPAFFLFSIVVAVPGVVMVWWLRDALARLGRPQTDGMVD
                     D"
     gene            3524..3841
                     /locus_tag="PKV97_05545"
     CDS             3524..3841
                     /locus_tag="PKV97_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05377.1"
                     /translation="MSHTVTVVFGGEREYEFPLRDADVASTTKEQARSWLAREFEDLE
                     CTPSNPMGKVLVLDMVLNVAKYGGESRFEQAGEWARKYALHTAVALDRPTVRVDVSSF
                     VVG"
     gene            complement(3855..5540)
                     /locus_tag="PKV97_05550"
     CDS             complement(3855..5540)
                     /locus_tag="PKV97_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05378.1"
                     /translation="MQENSQAVLVATGALPALILIAAALTLPVCLTLLALYRRAVLRS
                     MALASPAAGLGGSRRPQTAPAAPPAAALRLELCGANAAHDSPTLAALRRSLRAAGLVH
                     LLAGLACALVLTAAWMQFTWGDGGFVLVRFLLVFACHAWPAVLAVGLVTAGTTRQRLA
                     LGLAYVLLMLALAAWALARNPELSALDLARFWASTNLPPTVLLLAFLHRRIRAVGPLV
                     LAFMLVAVIGAEAAVRLAGQSEATMRLAIGVGGALGLDGTQTFWALLLVGAAVAALLG
                     RRVLKWLGRRYVARRSSDQLLTLEAMWLLFAVVQSVGFAFEGLAWLAAGPLAFLAWKL
                     TTVAGFRLAGLGHAQAPAPGLLLLRVFALGARSERLFDAFGKRWLRIGNIDMIAGPDL
                     ATTAVEPHEFLDFVGGRLSRAFVRDEADLARRHAARALGPDPDGRHRVNEFFCHDDTW
                     RPTMLCLARAADAVLMDLRGFSPQNEGCRYELQQLLDHVALERVVVLVGRDTDRGFLE
                     STLAALWQSSRAESPNRDNPGPLLRMVEERGDETAARLVEALLEAPPRKAAVA"
     gene            complement(5621..6211)
                     /gene="metW"
                     /locus_tag="PKV97_05555"
     CDS             complement(5621..6211)
                     /gene="metW"
                     /locus_tag="PKV97_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNB05379.1"
                     /translation="MTSYRYDYDVITQWIEPGEKVLDLGCGDGELLRHLMDVRQVQGY
                     GVENDPDKLLASVNNGVNVIQMDLEKGLVGLEDGFFDHVIMSLSLQAMHNTLGILAEM
                     LRVGREAVVSFPNFGYWRHRQSILNGRMPVSESLPHQWFNTPNVRFFTIADFDALCEM
                     NGIAVRERLAFDEGKLVLDEANFLASVAVYRLGRNG"
     gene            complement(6295..7422)
                     /locus_tag="PKV97_05560"
     CDS             complement(6295..7422)
                     /locus_tag="PKV97_05560"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNB05380.1"
                     /translation="MIAPQSVGVVVPQRAEFTTPLALRSGGTLNNYHLVYETYGTLNA
                     DRSNAVLVCHALSGSHHVAGTYADAPHNVGWWDNLIGPGKPLDTRKFFVIGVNNLGGC
                     YGSSGPNQINPATGKLWGADFPFVTVEDWVESQARLADRLGIERFAAVVGGSLGGMQA
                     MSWALQYPDRVGHVAVIAAAPKLTAQNIAFNEVARQAILSDPEFHGGHYYAHGVVPTR
                     GLKLARMVGHITYLSDDSMAEKFGRSLRHGRNTYSYDVEFEIESYLRYQGDKFAGYFD
                     ANTYLLTTKALDYFDPAFEYGGHLPAALARASADFLVISFTTDWRFSPERSREIVYAL
                     LHNKRNVSYAEIDCPAGHDSFLLDETRYHKLLSAWFDRIEV"
     gene            7618..7821
                     /gene="thiS"
                     /locus_tag="PKV97_05565"
     CDS             7618..7821
                     /gene="thiS"
                     /locus_tag="PKV97_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNB05381.1"
                     /translation="MIQVLLNGQPESLQPDLSVLALLEARGLVGKRLAVERNGEIVPK
                     ARHAEVKLADGDKLEIVVAVGGG"
     gene            8018..8803
                     /locus_tag="PKV97_05570"
     CDS             8018..8803
                     /locus_tag="PKV97_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNB05382.1"
                     /translation="MNDPLVIAGKAYHSRLLVGTGKYKDFAETREAIDASGAAIVTVA
                     IRRTNIGQNPGEPNLLDVLPPEKFTILPNTAGCYTADDAVRTLRLARELLDGHALVKL
                     EVLGDPKSLFPNMPETLKAAETLVKDGFDVMVYCADDPIQAKMLEDIGCVAIMPLASL
                     IGSGMGILNPWNLRLIIDNAKVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAGK
                     PVLMASAMRKAVEAGREAFLAGRMPRKFYTADPSSPTQGLIGS"
     gene            8800..9543
                     /gene="trmB"
                     /locus_tag="PKV97_05575"
     CDS             8800..9543
                     /gene="trmB"
                     /locus_tag="PKV97_05575"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNB05383.1"
                     /translation="MSEVPDEPVAGVEIIGRDSPEHRFSSRSIRSFVLRQGRMSEAQQ
                     RYLDDMLPKVGIDYRAAPLDLDEAFGRKAPKILEIGFGMGETTAKIAAAMPDRDFLGI
                     EVHTPGVGALCKLIAEGHLGNLRIMQHDAVEVMRDMIGDGALAGVHLFFPDPWRKKRH
                     FKRRIVQPDFVALIARKLAPGGYFHCATDWEDYAHWMLGILSAEPALENTADGFAPRP
                     EHRPLTKFENRGLKLGHGVWDLVFRRRAG"
     gene            9569..>10999
                     /gene="sppA"
                     /locus_tag="PKV97_05580"
     CDS             9569..>10999
                     /gene="sppA"
                     /locus_tag="PKV97_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNB05384.1"
                     /translation="MIRGLFRFLLNVLRMFVRALDLVVRGVFYALLIFGLGVLVSFFF
                     HPEPEVQAGSALVLRPVGTIVEQAELEPPLALLRAGGAPAGQLRLADLVDAVRKARDD
                     ARIAALVIETDELVGGGFSKLAELRAAIADFKASGKPVLARGERFTQSQYYLASVADE
                     LHLSPDGFVLLRGLARYGTYFRDALDKLGVKVHVFRVGEYKSFSEPFTRSDMSDEDRE
                     ATRDLLDGLWRFMRDDIAASRKLAPAAIDAHVNDIRGALAAAGGDAAKAALAAGLVDR
                     FSTRDEWRARLIEAVGTDHEGKDVRTIEAEAYLALAADDTRHAAGSVAVIVAQGTIVD
                     GAEPAGVVAGDTFARLIREAREDEDIKALVLRIDSPGGSAWASELIRRELELTRQAGK
                     PVIASMSSVAASGGYWIATGADEIWAAPSTVTGSIGIFGLFPEFSEPLRRLGIGVDGV
                     ATAPLAGALDPRRPLDPAAAESMQLGI"
BASE COUNT         1784 a         3827 c         3870 g         1518 t
ORIGIN      
        1 tgcaggagcg gcgcaagccg cgaacgccac ccatcgaccg atcggcgccg gctagctgcg
       61 ccagaccccc agctccgtca gctgcctcgt cgctgcctcg gcccagccgc ggttggcggc
      121 agggctggcg gggctggggt gcagcacgcg gccgaacttc accccgggca ggccggcaag
      181 cgcctcgctg gcgcgctttt ccgcccagtt gccgatgccg atcacccact cgggctgcag
      241 cgccccgacc aggatgcgca gatgcgcgtc gcaggccgcc agcagcgggc gctgctcggc
      301 caccggcagc ttgtcggggg tgaggttgcg gccaccctcg aagaacacta gcgggcagta
      361 gttggcgacg aagtgctcgg cgaagaaggc ctcgggtgcg ccatagcggg cgcggaacag
      421 gccccacagg cgctggccgc tgacctccga gcgcgcgcag gcgaagccct ccaccggccg
      481 tttcgggttg acgatgagcg gctgcccgac cgggccctcc agccccagcc agtcgcgcgc
      541 gctggcgatc tcgccgaagg gcacgccgat ctgcgccatg ccgaaggggc cggggttcat
      601 gccgacgaag accacccgcc ggcggccgtc gccatagcgc tcgaggtagc gtcggtgcac
      661 ctcccaggcg tagtcgagcg ggttgtacac gtgggtcacg ggcggagcga aggggagtgc
      721 gtccaccgcc gcggagagtt cgcgggcggc gtcgatcagg atgcgggcgg tcatgtcggg
      781 aagagcagct tgagcaggta gagggcggcc atgccggcgc cgatggtggc gagcacgttg
      841 cgcgtgcgcc aggccaccag caccgcgacc agcgccgccc aggggcgcgg ggtgtcgaag
      901 gcgaaaacga cttcgccgcc ggccacgaag agggcactcg cggtcagcgc ggcgaagctt
      961 gccggtgcga tgtgaaggca caaggcatgc agccagcgcg gcaggctgcg gctggcgaag
     1021 aggccgatca tcgcgtagcg gtacaggtag gtcagcaggc cgaccagcac cagcagcagg
     1081 agcagggctg tcgtgctcat cgtgcctgct ccagccggcg caccgtccac gcgccagcag
     1141 cgagcccgag cacgatcgcc gccaccaggc cgagcttgta gggcagggcg gcgaaggcga
     1201 gggcgccgag gccggccacc agcgctgcgg cctggcctgc gcgctcgcgc agcagcaggg
     1261 tgagcaaggc gatgaaggtg agcggcacgg cgaagtcgag ttcccattcg ggcgggatgg
     1321 tggtgccgaa cagcaggccg gcggcggtgg acaactgcca gcccgcccac agcgtgaagc
     1381 cggcgccgag gaagtaccag tggcgcaggt cggcggtggc gttcgtgtgc tcggcgtggt
     1441 ccgccgctgc cgcatgacgc tgctggtagc gcagcaccgc caccgcatag gcctcgtcgg
     1501 tgagcacgta ggccagcagc aggcgccagc gccgcggcag gtggcgcacg tattcggcca
     1561 tcgaggcgct gtagagcacg tggcgcaggt tgagcaggcc ggaggtgagc gcgatgggaa
     1621 tgaagcccgc gccggcggcc agcatctgca cgatgacgag ctgggcggcg ccggcgaaga
     1681 cgatggccga ggcgagctgc gccagccagg ccggcacccc ggcggcgagc gccgcgatgc
     1741 cgtagatcat gccgaagggc gccacgccga gcagcagcgg cgcctcgtcg cggcagccgg
     1801 cgaggaactc gcggcgtgcg ctcgtgccgg tgtgcgccat cagtcgtcca ccatgccgtc
     1861 ggtctgcggg cggccgaggc gcgcgagcgc gtcgcgcagc caccacacca tcaccacgcc
     1921 cggtacggcg accacgatcg agaagaggaa gaaggccggc cagccgatgc tctgcgacag
     1981 cacgcccgac agcgggctga cgtagatccg ccccactgcg gcgaaggccg acagcatggc
     2041 gtagtgggtc gccgtgaagc gctggttgca cagccccatc agcagcgcga cgaaggccac
     2101 cgtgcccatg ccgccgctga tgttctcgcc ggcgatcacg gtcagcagca gccggtcgag
     2161 cgcggccggc gtgtcgatcg ccacgaagcc ccagtcgaag gccggcacca tgactgcgcc
     2221 ccaggcgccc ttgccgagct ctgcgagcag gtagaagccg aagttggaca ccagctgcag
     2281 cacgccgaac agcagcagcg agcggtacag cgccagccgc gtcatgatca gcccgccgat
     2341 gaaggcgccg aggatggtca gccagatgcc gatcaccttg ttggcgatgc cgacctcttc
     2401 ctgcgagaag cccatgccct tgatcaggaa gggcgtggtc aggctgccgg cgaaggcgtc
     2461 gccgagcttg tagaggatga tcagcgccag gaaggccgcc gcgccctgct gcgcgaagta
     2521 gctcgacagc gagcggttga gcgtctcgaa gccggcacga cgcgccgccc agcccgccag
     2581 cggcagcgcg agcgcgatct ccgccatcac gaacaggagc tggatccagc ggttggcgtc
     2641 gttcgggtcg agcccgagca ggatcagcgc ctggcgggcg ctccagtagc cggcggcgac
     2701 cccggcgagc atcgcggcga agccgagcag ctcgcgcctg gggtcggaat cgagcggctt
     2761 cagcgccgcc gacacgcgcg gcagcgccag cagcgacacc accgcgcagg ccgccatgat
     2821 cagcgccatg gtctcgtata cccgcggcca cgacgcccac tgcccggccc agatcagcgc
     2881 gatgccgcca gacaggatca tcgccaggcg gtaggcgaag acatgcaccg acgcgcccag
     2941 cccgcgctcg gcctcgggca gcaggtcggt gcggtaggcg tccaccacca cgtcctgcga
     3001 ggccgagagg aaggcgatcg ccaccgccgc gatggcgaac aggcccggcg tggccgtcgg
     3061 cgacaggctc gccatccacc acagcagcgc cgccagcgcg agctgcgtca gcgccagcca
     3121 gccgcgccgg cgccccagcc agggcggctc gaagcggtcc atcagcggcg cccacaggaa
     3181 cttgaaggta tacgggacgc cgaccaggcc gaaaaaaccg atcgtggcga ggtccacgcc
     3241 gtcgactgtc agccaggcct gcatggcctg gccggtcagc gccaacggca ggccggaggc
     3301 gaagccgagc accaggatgg cggacaggcg ccaggcacgc gcgaacgcag gagagggagg
     3361 tgcggacaag gaggagcggc cggggtgttg cagggccgcg cattatcgca gatcatccgc
     3421 gaccccccgg tttacacaat tcgatacttt gcttgcgtct gaaacgttct gactcaacaa
     3481 tgccggcaaa gcaaaattcc ccgattcgtg gaggtcgttt ttcatgtccc acaccgtgac
     3541 cgtggtcttc ggaggcgagc gcgagtacga gttcccgctg cgcgatgccg acgtcgcctc
     3601 gaccaccaag gagcaggccc gcagctggct cgccagggaa ttcgaggatc tcgagtgcac
     3661 cccgtccaac ccgatgggca aggtgctcgt cctcgatatg gtgctcaacg ttgccaagta
     3721 cggcggcgag agccgtttcg agcaggctgg cgaatgggcg cgcaagtacg cgctccatac
     3781 cgcggtcgcg ctcgatcgtc cgaccgtgcg ggtggacgtc tcgtccttcg tggtcggctg
     3841 atcctttcgt tcgttcaagc caccgccgcc ttccgcggcg gtgcttccag caaggcctcg
     3901 accaggcgcg cggcggtctc gtccccgcgc tcctccacca tgcgcagcag tgggcccggg
     3961 ttgtcgcggt tgggggactc tgcccgactc gactgccata gcgcggcgag cgtgctttcc
     4021 agaaagccgc ggtcggtatc gcgcccgacc agcaccacca cgcgctccag cgccacgtgg
     4081 tcgagcagct gctgcagctc gtagcggcag ccctcgttct gcggcgagaa gccgcgcagg
     4141 tccatcaata ccgcatccgc cgcccgcgcc aggcacagca tggtcgggcg ccaggtgtcg
     4201 tcgtggcaga agaactcgtt gacgcggtgg cggccgtccg ggtccggccc cagcgcgcgt
     4261 gccgcatggc gccgcgcgag gtcggcctcg tcgcgcacga aggcgcgcga cagccgtccg
     4321 ccgacgaaat ccaggaactc gtgcggctcc accgcggtgg tggcgaggtc ggggccggcg
     4381 atcatgtcga tgttgccgat gcgcagccag cgcttgccga aggcgtcgaa gaggcgctcg
     4441 ctgcgcgccc cgagcgcgaa cacgcgcagc agcagcagcc cgggtgccgg ggcttgcgcg
     4501 tgcccgagcc cggcgaggcg gaagccggcc accgtcgtca gcttccaggc gaggaaggcg
     4561 agcgggccgg ccgcgagcca ggcgagcccc tcgaaggcga agcccaccga ctgcacgacc
     4621 gcgaacagca gccacatcgc ctccagggtg aggagctggt cgctgctgcg gcgtgcgacg
     4681 tagcgcctgc ccaaccactt cagcacccgc cggccgagca gtgcggcgac ggcggccccg
     4741 acgagcagga gtgcccagaa ggtctgcgtg ccgtcgaggc cgagcgcgcc gccgacgccg
     4801 atcgccaggc gcatcgtcgc ctcgctctgc cccgccagcc ggaccgccgc ctctgcgccg
     4861 atcaccgcga ccagcatgaa ggcgagcacc agcggtccta ccgcacggat gcgccggtgc
     4921 aggaaggcga gcagcagcac ggtcggcggc aggttggtgc tggcccagaa acgcgcgaga
     4981 tcgagcgcgg aaagctccgg gttgcgcgcc agcgcccacg cggcgagggc gagcatgagc
     5041 aggacgtagg ccagtcccag cgccaggcgt tgtcgcgtcg tgcccgccgt caccagcccg
     5101 accgcgagca cggccggcca cgcgtgacag gcgaacacca gcaggaagcg gaccagcacg
     5161 aagccgccgt ccccccaggt gaactgcatc caggcggcgg tgagcaccag ggcgcaggcg
     5221 agcccggcga gcagatggac gaggccggcc gcgcgcaaag atcgccgcag cgccgccagc
     5281 gtcgggctgt cgtgcgcggc gtttgccccg cacaactcca ggcgcagcgc tgccgctggt
     5341 ggtgccgcgg gggcggtctg cggccggcgc gagccgccga gccccgcggc cgggctggcg
     5401 agcgccatcg agcgcagcac cgcccgtcgg tacaaggcga gcagcgtcag gcacaccggc
     5461 aaggtcagcg ccgcggcgat caggatcaac gcgggaagcg cgccggtggc gacgaggacg
     5521 gcctggctgt tctcctgcat cgctgcgcgt ctcctgtgcc cgtgaccgca gcgcggtcac
     5581 ggttcagtat gggagtggga ggcccgcggc agcgggcgcc tcagccgttg cgtcccaggc
     5641 ggtacaccgc cacgctggcg aggaagttgg cctcgtcgag taccagcttg ccttcgtcga
     5701 aggcgaggcg ctcgcgcacc gcgatgccgt tcatctcgca cagcgcgtcg aagtcggcga
     5761 tggtgaagaa gcgcacgttg ggggtgttga accactggtg cgggaggctc tccgacaccg
     5821 gcatgcggcc gttgaggatg ctctggcggt ggcgccagta gccgaagttg ggaaagctca
     5881 ccacggcctc gcggcccacg cgcagcatct cggcgagaat gcccagcgtg ttgtgcatgg
     5941 cctgcagcga caggctcatg atcacatggt cgaagaagcc gtcctccagg ccgacgaggc
     6001 ccttttcgag gtccatctgg atcacgttga cgccgttatt gacgctggcg agcagcttgt
     6061 cggggtcgtt ctcgacgccg tagccctgca cctggcgcac gtccatcagg tggcgcagca
     6121 gttcgccgtc gccgcagccc aggtcgagca ccttctcgcc gggctcgatc cactgggtga
     6181 tcacgtcgta gtcgtagcga taggaagtca tgtcagtgtg cgccgagccg tctcaagcgt
     6241 actgacgccc cctcgggggg cagcgaacgc agtgagcgtg ggggttgtat caatttaaac
     6301 ctcgatgcgg tcgaaccatg ccgacagcag cttgtggtag cgcgtctcgt cgagcaggaa
     6361 ggagtcgtgg ccggccgggc agtcgatctc ggcgtagctg acgttgcgct tgttgtgcag
     6421 cagcgcgtag acgatctcgc gcgaacgctc gggcgagaag cgccagtcgg tggtgaagga
     6481 aatcaccagg aagtcggcgc tggcgcgggc aagcgccgcg ggcaggtggc cgccatactc
     6541 gaaggcgggg tcgaagtagt cgagcgcctt ggtggtcagc aggtaggtgt tggcgtcgaa
     6601 gtagccggcg aacttgtcgc cctggtagcg caggtaggac tcgatctcga attcgacgtc
     6661 gtagctgtag gtgttgcggc cgtggcgcag gctgcggccg aatttttccg ccatcgagtc
     6721 gtcggacagg taggtgatgt gacccaccat gcgcgccagc ttgaggccac gcgtcggcac
     6781 cacgccgtgc gcatagtagt ggccgccgtg gaactcgggg tcgctcagga tggcctggcg
     6841 ggcgacctcg ttgaaggcga tgttctgcgc ggtgagcttg ggcgcggcgg cgatcaccgc
     6901 gacgtggccg acgcggtccg ggtactgcag cgcccacgac atcgcctgca tgccgccgag
     6961 cgagccgccg accaccgcgg cgaagcgctc gatgccgagc cggtcggcca ggcgcgcctg
     7021 cgattcgacc cagtcttcca cggtgacgaa ggggaagtcc gccccccaca gcttgccggt
     7081 ggcggggttg atctggttgg ggccagagga gccgtagcag ccgccgaggt tgttcacccc
     7141 gatcacgaag aacttgcgcg tgtcgagcgg cttgcccggc ccgatcaggt tgtcccacca
     7201 gccgacgttg tgcggcgcgt cggcgtaggt gccggcgacg tggtgcgagc ccgacagcgc
     7261 atggcacacc agcaccgcgt tgctgcggtc cgcattgagc gtgccgtagg tttcgtagac
     7321 gaggtggtag ttgttcagcg tgccgccgct gcgcagcgcg agcggcgtgg tgaactccgc
     7381 ccgctgcggc acgaccacgc cgaccgattg gggtgcgatc attttcgtca tctgcaaaac
     7441 aaaaaaccca gccggctgca aacgcggact gggtgccctc gctttagccg tatttgttga
     7501 gcgcccgcaa gctgttgatc aaatcggcgc taggtggctg gaacttagcg cccgcgaagg
     7561 gaaaagtcaa acggctggcg tgataagatt ttcggctccc gaacagcctc gaacgccatg
     7621 atccaggtcc tcctcaacgg tcagcccgaa tcgctccagc ccgacctctc ggtgcttgcc
     7681 ctgctcgagg cgcgcggcct cgtcggcaag cgcctcgcgg tcgagcgcaa cggcgagatc
     7741 gtgcccaagg cccgccacgc cgaggtgaag ctcgccgacg gcgacaagct cgaaatcgtc
     7801 gtcgcggtcg gcggcggctg aagcccccga cctgcgtgtc ccgtaggagc ggcgcaagcc
     7861 gcgattacgg ccgggcggcg gcgaccgcgc tccctcagca ccgcgccgag gtccggcgcc
     7921 ccgatcgcgg cttgcgccgc tcctgcaggg tcctgcaccg tgcggcggtg cctcgactgg
     7981 ctcccattcc tcagacaaac ccttacaggc tcccaacatg aacgaccccc tggtcatcgc
     8041 cggcaaggcc taccactccc gcctgcttgt cggcaccggc aagtacaagg acttcgccga
     8101 gacgcgcgag gccatcgacg ccagcggcgc tgccatcgtc accgtggcca tccgccgcac
     8161 caacatcggc cagaacccgg gcgaacccaa cctgctcgac gtgctgccgc cggagaaatt
     8221 caccatcctg cccaacaccg ccggctgcta caccgccgac gacgcggtgc gcacgctgcg
     8281 ccttgcgcgt gagctgctcg atggccacgc gctggtcaag ctggaggtgc tcggcgaccc
     8341 caagagcctg ttccccaaca tgcccgagac gctcaaggcc gccgagaccc tggtcaagga
     8401 cggcttcgac gtcatggtct actgcgccga cgaccccatc caggcgaaga tgctggaaga
     8461 catcggctgc gtcgcgatca tgccgctggc ctcgctgatc ggctccggca tgggcatcct
     8521 caacccgtgg aacctgcgcc tgatcatcga caacgccaag gtgccggtgc tggtcgacgc
     8581 cggcgtcggc accgcatcgg atgccgcgat cgcgatggag ctgggctgcg acggcgtgct
     8641 gatgaacacc gcgatcgccg ccgccggcaa gccggtgctg atggcgagcg cgatgcgcaa
     8701 ggcggtcgag gccggccgcg aggccttcct cgccgggcgc atgccgcgca agttctacac
     8761 cgccgacccg agctcgccga cccaggggtt gatcggttca tgagcgaagt gcctgacgaa
     8821 cccgtcgccg gtgtcgagat catcggccgc gacagccccg agcaccgctt ctccagccgc
     8881 agcatccgca gcttcgtgct gcgccagggg cgcatgtccg aggcgcagca gcgctatctc
     8941 gacgacatgc tgccgaaggt cggcatcgac taccgcgccg cgccgctcga tctggacgag
     9001 gccttcggcc gcaaggcgcc gaagatcctc gagatcggct tcggcatggg cgagaccacg
     9061 gcgaagatcg ccgcggcgat gccggatcgc gacttcctcg gcatcgaggt gcatacgccc
     9121 ggcgtcggcg cgctgtgcaa gctgatcgcg gaaggccatc tgggcaacct gcgcatcatg
     9181 cagcacgacg cggtcgaggt gatgcgcgac atgatcggcg acggcgcgct cgccggcgtg
     9241 catctgtttt tccccgaccc ctggcgcaag aagcgccact tcaagcgccg catcgtgcag
     9301 cccgacttcg tcgccctgat cgcgcgcaag ctcgcgcccg gcggctactt ccactgcgcc
     9361 accgactggg aggactacgc gcactggatg ctcgggatcc tctccgccga gccggcgctg
     9421 gagaataccg ccgacggctt cgccccgcgt cccgagcatc gtccgctgac gaaattcgaa
     9481 aaccgtggtc tgaagctggg acacggggta tgggacctcg tcttccgccg ccgcgcgggc
     9541 tgaaccatcc gtcacgagga caagcagcat gatccggggc ctgttccgct tcctattgaa
     9601 cgtgctgcgc atgttcgtgc gcgcgctcga cctcgtcgtg cgcggcgtct tctacgccct
     9661 gctgatcttc ggcctcggtg tgctggtgtc cttcttcttc caccccgagc ccgaggtgca
     9721 agccgggtcc gcgctggtgc tgcgaccggt gggcacgatc gtcgagcagg ccgagctcga
     9781 gccaccgctg gcgctgctgc gcgccggggg cgcaccggcc ggccaactgc gtctggcgga
     9841 tctggtggac gcggtgcgca aggcgcgcga cgacgcgcgc atcgccgcgc tggtgatcga
     9901 gaccgacgag ctggtcggcg gcggcttctc caagcttgcc gagctgcgcg ccgcgatcgc
     9961 cgatttcaag gcctcgggca agccggtgct ggcgcgcggc gagcgcttca cccagtcgca
    10021 gtactacctc gcctcggtgg ccgacgaact ccacctgtcg ccggacggct tcgtgctgct
    10081 gcgcggcctg gcgcgctacg gcacctactt ccgcgacgcg ctcgacaagc tcggggtcaa
    10141 ggtgcacgtg ttccgcgtcg gcgagtacaa gtccttctcc gagcccttca cccgcagcga
    10201 catgtccgac gaggaccgcg aggccacccg cgacctcctc gacgggctgt ggcgcttcat
    10261 gcgcgacgac atcgccgcca gccgcaagct cgcgcccgcg gcgatcgacg cgcatgtgaa
    10321 cgacatccgc ggcgcgctcg ccgccgccgg gggcgatgcc gccaaggccg cgctcgccgc
    10381 cggcctggtg gaccgcttca gcacccgcga tgaatggcgc gcccgcctga tcgaggccgt
    10441 cggcaccgac cacgagggca aggacgtgcg caccatcgag gccgaggcct atctcgcgct
    10501 cgctgcggac gacacccgtc acgccgccgg cagcgtggcg gtgatcgtcg cccagggcac
    10561 catcgtcgat ggcgccgagc cggccggcgt ggtcgccggc gacaccttcg cccgcctgat
    10621 ccgcgaggcg cgcgaggacg aggacatcaa ggcgctggtg ctgcgcatcg acagcccggg
    10681 cggcagcgcc tgggcctcgg agctcatccg tcgcgaactc gaactcaccc gccaggccgg
    10741 caagccggtg atcgcgtcga tgagctcggt ggcggcctcg ggcggctact ggatcgccac
    10801 cggtgccgac gagatctggg cggcgccttc cacggtgacc ggctcgatcg gcatcttcgg
    10861 cctcttcccg gagttctccg agccgctgcg ccgcctcggc atcggcgtcg atggcgtcgc
    10921 caccgcgccg ctcgccggcg cgctggaccc gcgtcgcccg ctcgacccgg ccgcggccga
    10981 atccatgcag ctcggcatc
//
LOCUS       DAOQNN010000063        10882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2804_length_10882_cov_9.791170, whole genome shotgun sequence.
ACCESSION   DAOQNN010000063 DAOQNN010000000
VERSION     DAOQNN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10882
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2804_length_10882_cov_9.791170"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..5687
                     /locus_tag="PKV97_05585"
     CDS             24..5687
                     /locus_tag="PKV97_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB05385.1"
                     /translation="MSYAKSVRLPCRLLPEAVSAVVLKLLAKDASERYQSAHGIVHDL
                     RECLQRREQPAAHDFVLASRDVSARFQVPRRLYGRTAQLAELEGRVEACAAGGRAIVL
                     ISGYTGVGKSSLVHELRRAVLERHGHFASGKFDQYRRNPPHSALLQALRELVRQHLTE
                     PGERLAALGSRLREQLGGYLGTLVRLLPELGLVVPDAGTSPAGQHRFDEQGRLHLFSR
                     LIDALTEPGQALTLFLDDLQWADPASLGLIESLATHAGLPRLLLVGSYRHNEIGPGHA
                     LAGFVEHLRRSALPPVELRLQPLDLAQVRQLLADTLRCSPIECARLAEACHEKTQGNP
                     FFLNQLLDAVHEEGLIRFHGQRGEWDEAAIRARALSTDVVELMVERLRRLPERTRAVL
                     PLAACIGGTFDLKTLSIVAEGRARQLAHDLVPALGEGLVVPLDDGDRPERDRLGRARY
                     RFAHDRVQQTAYSLLADDAREQLHLRVGRLLRQNLDTGALDRRVFEIAGHLNLARSAI
                     DDPDERIALATLNLCAGLRARESAAFESALDYLDSGLHALPADAWRTQHRLCLDLHLA
                     AADAANSRGDGERMQALLDAAQEHAHAPLDRVRCEQIRIQAHVAHNRFGAGLEIARDT
                     LARLGVHLPAAPGRLRIAAELLRTRLLIRHTAPEELLHMPAATEPRVLAAQALLGGMF
                     GIVKFSSSALRPLVMARQVELALRHGHTDASAQALAGFGGVLCGVLGAIDEGYRAGTL
                     ALALEAARPSRQGRQRTLSLFNCYVRHYREPLQCALDGLREAHAEALACGDLEYAAYS
                     LAAAIQYAVPLADDLEALARRMRGQLVQLEQTGQQQSLQYSYMALQAVTRLCEPSASP
                     TALDGEHYREAAMLEAHRRGNHLTAVCLHHFYKALLALVHADYPAALAECTLGEAHLP
                     YVGGTYTATWFRSLHALALLRTLPPPGNARRRALHTLRRLVRHVRMQARHAPANHAHR
                     VALIEAERLRVRARPGAAAAYDRAIALAEQGGFWLDAALACEFAARFHLDAGERAVAR
                     SCFDDACRRYQRLGAHSPVVHLHEQLEEYGLWPRPPGLAGAGEMQGGLDETNHSFDLS
                     SVIEASQAISDEIVLERLIERLLQLALTSAGAQRGALALSRDRHLRVEAIAGLEEGST
                     LMCSLALEAATSRVPVSVLNYVARTGEALVLGDAPRQQMFAHDPYIRSQAPRSLLCMP
                     ILYHGELTALLYLEHRLGRDIFDQQRLKTLRILAAQAAISIENAKLYLGLQQSEQAYR
                     SLYENAIEGIFRVDPDGRFLSANPALVRLLGHDSAAEFLTGITDVSTQCFARAEDQRR
                     FLGRLNMEEKVVGFETRWRRRDGSEVEVSISARRVLDDGGRLRCYEGSLTDIGERKAK
                     ERAELARERAEAASRAKSDFLAAMSHEIRTPMNGILGMAQLLARSGLAPGQAEWVDAI
                     RASGSTLLGILDDVLDFSKIEAGQLTLESAPFSPAEALAALRPMLATMSAQKGLVLRL
                     EIDPALPGGVLGDRRALHQIVLNLAGNAVKFTARGFVAVRARCLPARAGRCRLGLEVE
                     DSGIGIAEEAQRRIFTEFTQADGSITRRFGGTGLGLAICRRLVDLQGGSLGVRSRPGE
                     GSTFSCELEYPLATPAPRASPRPHAAIGEARLHVLVVEDVELNRCIAGTLLEDEGHTV
                     RFAADGYGALELHEREDFDTILLDIHLPDLDGMEVARRIRRHPDARKARARIVALTAS
                     ITTDEVARYRAAGVDAVVGKPFDFDELIRSLREPPVPPAAAPGSALMDTRLLATHFAR
                     LGDERVRPMMDMLATQGRAGLAEFAASADPATQRERLHRLVGMAANLGLAAFATRCRE
                     LEATPLPADLRAQAGALRTLFEDSIAALHAFDPRDAQAALEAAAR"
     gene            5856..7277
                     /locus_tag="PKV97_05590"
     CDS             5856..7277
                     /locus_tag="PKV97_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB05386.1"
                     /translation="MNKTPSLVIGGRKVAGDRGTLPVIDPALGEAFAECPNASPAQLD
                     EAVAAAARAFEQWQHSSCAERRALLEAIAARIEQNAPELAEIIVREQGKPLALAHMEV
                     GGAVAWTRATAALELPVEVIEDRPGKRIELHRRPLGVVGSITPWNWPLMIAVWHIMPA
                     LRAGNAVVIKPSELTPLNTLRLVELIDEVAPPGLVNAVAGGAALGRGISGHPGIHKIV
                     FTGSTRTGQDIMRNAADTLKRLTLELGGNDAGIVLPGTDIGAIAEGVFGSAFLNMGQT
                     CAALKRLYVHESQYEDMCRHLVAIAARQKLGSGLDEGTSFGPIQNRDQFERVCELVED
                     ARAAGARILCGGEPLPGKGYFYPPTIVADIADGTRLVDEEQFGPVLPVIRYRDVDEAL
                     RLANASTNGLGGSVWSGDLEAARALANRLECGTVWINGHAEVLPHCPFGGCKMSGFGV
                     EFGLEGLLEYTRPQLFNINLPAA"
     gene            7278..8252
                     /locus_tag="PKV97_05595"
     CDS             7278..8252
                     /locus_tag="PKV97_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05387.1"
                     /translation="MSAPLGFLRRPSAPASASVAASTASPPSLAALPATGVAIAVAMV
                     RPRALEHLGADTPARAAFWSATLHDEALAAWLAPLRPTRLRVGSEFCERLLPEPAQLA
                     RTLAEASVAGLEVCLLTPVASPEVLRALDALFALLPPGAEVVVNDWGVALRLRERHPL
                     LQAVAGRILCRMLKDPRLGNPQYAPPAAHGFASPWTRRLLARLGIARVEIDLPLFASA
                     DSFAGVPLPLGVHLPFAFVAKGRMCRTAGLAREGPERFAARPFCHHECLGLAARTERT
                     RDGTHDAWETLHVGNTVFCRHSAANLATLASAVAAGRVARLIVPGETL"
     gene            8263..9318
                     /locus_tag="PKV97_05600"
     CDS             8263..9318
                     /locus_tag="PKV97_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNB05388.1"
                     /translation="MKIVAPIRQLDEIAALARAGADELYCGVTPREWAERFGGASANR
                     RPGGNLPSLAALAEAVALAHRNGVQLSLVLNAQQYSVEQIEFALAIAHRYVDMGGDAV
                     IASDPGLLLALAEAEPELRIHVSSVATCRNADGARLYRELGARRLILPRDITLDEAAE
                     IAAAVPDLEIEAFVLNDGCVYEEGSCNTLHLPGALGGPICLDRYAYAHRHRDGRPLSA
                     ALAARLQENDEAYRRWLWYRFSCGFTTTADGLPFGPCGLCAIPAFGRGGIHALKIAGR
                     EGPPERKLASVRMVRRILDAHDNGEAPAAVMARARNLRPAHEHCATGFMCYYPEVVSR
                     ASEAAQPLCDGRAAGAQ"
     gene            complement(9312..9920)
                     /locus_tag="PKV97_05605"
     CDS             complement(9312..9920)
                     /locus_tag="PKV97_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNB05389.1"
                     /translation="MPNVTAVMSTPNPLANKFMLDAPLYTGAPRHFSAGVAVVGDTLG
                     ESLLAVPGVVDIYCTGGFITITRDPGTPWSAIEPAVTELIEGHKARRVIGIANPAGPG
                     GAAGAAIGAGEAVAEEPVEVEADDAELLMRINQILDEHIRPFLDQDGGGLDVVKLKEF
                     TLTVRYKGACGGCPSSSTGTLFAINNLLQNYVDDRLLVEPIY"
     gene            10107..10688
                     /gene="rsxA"
                     /locus_tag="PKV97_05610"
     CDS             10107..10688
                     /gene="rsxA"
                     /locus_tag="PKV97_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNB05390.1"
                     /translation="MKEYLLLLLGTALVNNVILVRFLGLCPFLGVSGKLETALGMGLA
                     TTFVLTLTSGAAWVVEHALLQPLDMLHLRILTYILVIAAVVQFTEMAIQKASPVLHHT
                     LGIYLPLITTNCAVLGTALLNTQQGNGLVASMLYGAGSALGFTLVILLFAGLRERIAL
                     ANVPAAFAGAPIALITAGLLSLAFMGFAGLPVR"
BASE COUNT         1522 a         4183 c         3655 g         1522 t
ORIGIN      
        1 atgaaagtta acgagttatt gatttgagct atgcgaagtc ggtgcgattg ccctgccgcc
       61 tgctccccga ggcggtctcc gccgtggtcc tgaagctgct cgccaaggac gcctccgagc
      121 gctaccagtc ggcgcacggc atcgtgcacg acctgcgcga gtgcctgcag cggcgggaac
      181 agccggcggc gcacgacttc gtgctcgcca gccgcgacgt ctccgcgcgc ttccaggtgc
      241 ccaggcggct ctacggccgc acggcccagc tcgccgaact cgagggtcgg gtcgaggcgt
      301 gcgccgccgg ggggcgcgcg atcgtgctga tcagcggcta caccggggtc ggcaagtcca
      361 gcctggtgca cgagttgcgc cgtgccgtcc tcgagcgcca cggccacttc gccagcggca
      421 agttcgacca gtaccgccgc aacccacccc actctgcact gctgcaggcg ctgcgcgagc
      481 tcgtccgtca gcatctcacc gagccgggcg aacgcctcgc ggccctcggc agccgtctgc
      541 gcgagcagct cggcggctac ctcggcacgc tggtgcgcct gctgcccgaa ctcggcctcg
      601 tcgtgcccga cgccggcacg tcgccggccg gccagcaccg cttcgacgag cagggccgcc
      661 tgcatctgtt ctcgcgcctg atcgacgcgc tcaccgagcc cggacaggcg ctcacgcttt
      721 tcctcgacga cctgcaatgg gccgacccgg cctcgctcgg actcatcgag tcgctcgcga
      781 cccacgccgg gctgccgcgc ctgctgctcg tcggcagcta ccggcacaac gagatcgggc
      841 cgggccatgc gctcgccggg ttcgtcgagc acctgcgcag gagcgcgctg ccgccggtgg
      901 aactgcgcct gcagccgctc gacctcgcgc aggtccgcca gctgctcgcc gacaccttgc
      961 gctgcagccc gatcgaatgc gcgcgcctgg ccgaagcctg ccacgagaag acgcagggca
     1021 atccgttctt cctcaaccag ctcctcgacg cggtgcacga ggaaggcctg atccgcttcc
     1081 acggccaacg cggggaatgg gacgaagcgg cgatccgcgc gcgcgcgctc agcaccgacg
     1141 tggtcgagct catggtcgag cgcctgcgcc gcctgcccga acgcacgcgc gcggtgctgc
     1201 cgctggcggc ctgcatcggc ggcaccttcg acctcaagac cctgtccatc gtcgccgagg
     1261 ggcgcgcgcg ccagctcgcg cacgacctcg tgccggcgct cggcgagggc ctcgtggtgc
     1321 cgctcgacga cggcgaccgc cccgagcgcg accgtctcgg gcgcgcgcgc tatcgcttcg
     1381 cgcacgaccg cgtgcagcag acggcctact cgctgctcgc ggacgacgcg cgcgagcagc
     1441 tgcacctgcg cgtcggccgc ctgctgcgcc agaacctcga cacgggcgcg ctcgaccgcc
     1501 gggtcttcga gatcgccggc cacctcaacc tcgcgcgcag cgccatcgac gaccccgacg
     1561 agcgcatcgc gctcgccacc ctcaacctgt gcgccgggct gcgcgcgcgc gaatccgccg
     1621 ccttcgagtc ggccctcgac tatctcgaca gcggcctcca cgcgctgccc gcggacgcct
     1681 ggcgcacgca gcaccgactg tgcctcgacc tgcacctcgc cgccgcggat gcggccaaca
     1741 gccgcggcga cggcgagcgc atgcaggccc tgctcgatgc cgcccaggag cacgcgcacg
     1801 ctccgctcga ccgcgtacgc tgcgagcaga tccgcatcca ggcgcacgtc gcgcacaacc
     1861 gcttcggcgc cgggctggag atcgcgcgcg acaccctcgc gcgtctgggc gtacatcttc
     1921 ccgcggcgcc cggccgcctg cgcatcgcgg cagaactgct gcgcacccgc ctgctgatcc
     1981 gccacaccgc gccggaggaa ctgctgcaca tgcccgcagc gaccgagcca cgcgtgctcg
     2041 ccgcgcaggc gctgctcgga ggcatgttcg gcatcgtcaa gttctccagc tccgccctgc
     2101 gcccgctggt gatggcgcgc caggtcgagc tcgcgctgcg ccacggccac accgacgcca
     2161 gcgcccaggc cctcgccggc ttcggcggcg tgctctgcgg cgtgctcggc gcgatcgacg
     2221 agggctaccg cgccggcacg ctggcactcg cactggaggc ggcgcgccct tcgcgccagg
     2281 ggcgccagcg caccttgtcg ctgttcaact gctacgtgcg ccactaccgc gaaccgctgc
     2341 agtgcgccct cgacggcctg cgcgaggcac acgccgaggc gctcgcctgc ggcgatctcg
     2401 agtacgccgc gtacagcctc gccgccgcga tccagtacgc ggtgccgctc gccgacgatc
     2461 tcgaggccct cgcccggcgc atgcgggggc agttggtgca gctcgaacag accgggcagc
     2521 agcagtccct gcaatacagc tacatggcgc tgcaggcggt cacccggctg tgcgagccgt
     2581 cggcctcgcc caccgcgctc gacggcgagc attaccgcga ggcggcgatg ctcgaggcgc
     2641 atcgccgcgg gaaccacctc accgcggtct gcctgcacca cttctacaag gccttgctcg
     2701 cgctggtgca tgcggactat cccgccgcac tcgccgaatg caccctgggc gaagcccacc
     2761 tgccctatgt cggcggcacc tataccgcga cctggttccg cagcctgcat gcgctcgcgc
     2821 tgctgcgcac cctgccgcca ccgggcaatg cgcgccggcg cgcgctgcac acgctgcgac
     2881 gcctggtgcg ccacgtgcgc atgcaggcga ggcatgcgcc cgccaaccac gcccaccgcg
     2941 tggcgctgat cgaggccgaa cgcctgcggg tgcgcgcccg ccccggggca gcggcggcct
     3001 acgatcgcgc catcgcgctc gcggagcagg gcggcttctg gctcgacgcc gcgctcgcct
     3061 gcgagttcgc cgcgcgcttc cacctcgatg ccggcgaacg cgcggtggcg cgcagctgct
     3121 tcgacgacgc ctgccgacgc taccagcgcc tcggcgccca ctcccccgtc gtccatctgc
     3181 acgagcagct cgaggaatac ggcctgtggc cccgaccgcc ggggctcgcg ggcgcgggcg
     3241 aaatgcaggg cggcctcgac gagaccaacc attccttcga cctctcctcg gtcatcgagg
     3301 cctcgcaagc gatttcggac gagatcgtgc tcgagcgcct gatcgagcgc ctgctgcagc
     3361 tcgcgctgac gagcgcgggc gcccagcgcg gcgcgctcgc cttgagccgc gaccggcatc
     3421 tgcgcgtcga ggcgatcgcc gggctggaag agggctcgac gctgatgtgc agcctcgccc
     3481 tcgaagccgc gacgagccgc gtgccggtca gcgtgctcaa ctacgtcgcc cgcaccggcg
     3541 aggcgctggt gctgggcgac gccccgcgcc agcagatgtt cgcgcacgac ccctacatcc
     3601 gcagccaggc gccgcgctcc ttgctgtgca tgcccatcct ctaccacggc gagctcaccg
     3661 ccctgctcta cctcgagcac cgcctcggcc gcgacatctt cgaccagcaa cgcctgaaga
     3721 cgctgcgcat cctcgccgcg caggcggcaa tctcgatcga gaacgccaag ctctacctcg
     3781 gcctgcagca atccgagcag gcctaccgct cgctgtacga gaacgcgatc gagggcatct
     3841 tccgggtcga tcccgacggg cgcttcctgt ccgccaaccc cgcgctggtc cgcctcctcg
     3901 gccacgacag cgcggcagag ttcctcaccg gcatcaccga cgtcagcacg cagtgcttcg
     3961 cccgcgccga ggaccagcgc cgcttcctcg gccgcctcaa catggaggag aaggtggtcg
     4021 gcttcgagac gcgctggcgc cggcgcgacg gcagcgaggt cgaggtctcc atctcggccc
     4081 gccgcgtgct cgacgacggc gggcggctgc gctgctacga gggctcgctc accgacatcg
     4141 gcgaacgcaa ggccaaggag cgcgccgaac tcgcacgcga gcgcgccgag gccgccagcc
     4201 gcgccaagag cgacttcctc gccgcgatga gccacgagat ccgcacgccg atgaacggca
     4261 tcctcggcat ggcgcagctg ctcgcgcgct ccgggctggc gccgggccag gccgaatggg
     4321 tggacgcgat ccgggcctcg ggcagcacgc tgctcgggat cctggacgac gtgctcgact
     4381 tcagcaagat cgaggccggg cagctcacgc tcgagtccgc gcccttctcg ccggccgagg
     4441 cgctcgccgc cctgcgtccg atgctggcga cgatgagcgc acagaaaggg ctagtgctgc
     4501 gcctggagat cgatccggcg ctccccggag gggtgctcgg cgaccgtcgc gcactgcacc
     4561 agatcgtgct caacctggcc ggcaacgcgg tgaagttcac cgcgcgcggc ttcgtcgcgg
     4621 tgcgcgcgcg gtgcctgccg gcgcgcgcgg ggcgctgccg cctgggcctg gaggtcgagg
     4681 acagcggcat cggcatcgcc gaggaggcgc agcgccgcat cttcaccgaa ttcacccagg
     4741 ccgacggttc gatcacccgc cgcttcggcg gcaccggcct ggggctggcg atctgtcggc
     4801 gcctcgtcga cctgcagggt ggaagcctgg gcgtgcgcag ccgcccggga gagggcagca
     4861 ccttctcctg cgaactggaa taccccctcg caaccccggc gccacgggca tcgccgcgtc
     4921 cgcacgctgc catcggcgag gcgcgcctgc acgtgctggt ggtcgaggac gtggaactga
     4981 accgctgcat cgccggcacg ctgctcgagg acgagggcca caccgtgcgc ttcgcggccg
     5041 acggctacgg cgcgctcgag ctgcacgagc gcgaggattt cgacacgatc ctgctcgaca
     5101 tccaccttcc cgacctcgac ggcatggagg tcgcacgccg catccgccgc caccccgacg
     5161 cgcgcaaggc gcgcgcgcgc atcgtcgcgc tcaccgcttc gatcaccacc gacgaggtgg
     5221 cgcgttaccg cgcggccggc gtggacgcgg tcgtcggcaa gcccttcgac ttcgacgagc
     5281 tgatccgcag cctgcgcgag ccccccgtac cgcccgccgc ggcaccgggg agcgccctga
     5341 tggacacgcg cctgctcgcc acccatttcg cccgcctcgg cgacgagcgc gtgcggccga
     5401 tgatggacat gctcgccacc cagggccgcg ccgggctcgc cgagttcgcc gcgagcgccg
     5461 atcccgcgac ccaacgcgag cgcctgcacc ggctcgtcgg catggccgcg aacctgggcc
     5521 tcgccgcctt cgccacgcgt tgccgggagc tcgaggcgac gcccctcccg gccgacctgc
     5581 gcgcacaggc cggcgcgctg cggacgctgt tcgaggacag catcgcggcg ctgcacgcgt
     5641 tcgacccccg ggacgcgcaa gctgcgctcg aagccgcggc gcgctgaggc cgcccgcctc
     5701 gcgccgcgca cgctacaact gcgcacaatt ccgcacaaat cgtgaaaagc tggtttacag
     5761 cgccgggcta gtcttcccat cgacgcaggc agggccgcgg cagccgaccc gcgagcccgt
     5821 caccacgcct tccaactcct gccaggatcc gatccatgaa caagacgccc tccctcgtca
     5881 tcggcggccg caaggtcgcc ggcgaccgcg gcaccctgcc ggtcatcgac cccgcgctcg
     5941 gcgaggcctt cgccgagtgc ccgaacgcct cccccgcgca gcttgacgag gcggtggcgg
     6001 cggccgcgcg cgcgttcgag cagtggcagc acagctcctg cgcggaacgc cgcgccctgc
     6061 tcgaagcgat cgccgcgcgc atcgaacaga acgcgcccga gctcgccgag atcatcgtcc
     6121 gcgagcaggg caagccgctc gcgctcgcgc acatggaggt cggcggcgcg gtcgcatgga
     6181 cgcgcgccac cgccgcgctc gagctgccgg tcgaggtgat cgaggaccgc ccgggcaagc
     6241 gcatcgagct gcaccgcagg ccgctcggcg tggtgggatc gatcacgccg tggaactggc
     6301 cgctgatgat cgcggtgtgg cacatcatgc ccgcgctgcg cgccggcaac gcggtggtga
     6361 tcaagccctc ggagctgacc ccgctcaaca ccctgcgcct ggtcgagctg atcgacgagg
     6421 tggcgccgcc cgggctggtg aatgcggtgg ccggcggcgc agcgctcggg cgcgggatct
     6481 cgggccaccc cggcatccac aagatcgtgt tcaccggctc gacgcgcacc ggccaggaca
     6541 tcatgcgcaa cgccgccgac acgctgaagc ggctcaccct ggaactgggc ggcaatgacg
     6601 ccggcatcgt gctgccgggc accgacatcg gcgcgatcgc cgagggggtg ttcggcagcg
     6661 ccttcctcaa catgggacag acctgcgccg cgctcaagcg cctctatgtg cacgagtccc
     6721 agtacgagga catgtgccgg cacctggtcg ccatcgccgc gcggcagaag ctcggcagcg
     6781 gcctggacga gggcacgagc ttcgggccga tccagaaccg cgaccagttc gagcgcgtat
     6841 gcgagctcgt cgaggatgca cgcgccgccg gcgcccgcat cctgtgcggc ggcgagccgc
     6901 tgcccggcaa gggctacttc tacccgccga ccatcgtcgc cgacatcgcc gacggcaccc
     6961 ggctggtcga cgaggagcag ttcggcccgg tgctgccggt gatccgctac cgcgacgtgg
     7021 acgaggcgct gcggctcgcc aacgccagca ccaacggtct cggcggctcg gtgtggtcgg
     7081 gcgacctgga ggccgcgcgc gcgctggcga accgcctcga gtgcggcacg gtgtggatca
     7141 acggccatgc cgaggtattg ccgcactgcc cctttggcgg ctgcaagatg tcgggcttcg
     7201 gcgtcgagtt cggcctcgag gggctgctcg aatacacccg cccgcagctc ttcaacatca
     7261 acctccccgc cgcctgaatg tccgcaccgc tcggcttcct gcgccgcccg tccgcgccgg
     7321 caagcgcgag cgttgctgcg agcacggcgt cgcctccgtc cctggctgcg ctgccggcga
     7381 ccggggtggc gatcgcggtg gcgatggtgc gaccgcgcgc actggagcac ctgggtgcgg
     7441 acacgcccgc acgcgcggca ttctggtccg ccaccctgca cgacgaggcg ctcgcggcct
     7501 ggctggcacc gctgcgcccg acgcggctgc gcgtcggcag cgaattctgc gagcgcctgc
     7561 tgcccgagcc cgcgcagctc gcgcgcacgc tcgccgaggc gtccgtggcc gggctggagg
     7621 tctgcctgct cacgccggtg gcctcgcccg aggtcctgcg cgccctcgat gcgctcttcg
     7681 ccctgctgcc gccgggcgcg gaggtggtgg tcaacgactg gggggtggcg ctgcgcctgc
     7741 gcgagcgcca tccgctgctg caggccgtcg ccggccgcat cctgtgccgc atgctcaagg
     7801 acccgcgcct cggcaacccg cagtacgcac cgcccgccgc gcacggcttc gcctcgccct
     7861 ggacccggcg cctgctcgcc cgcctgggga tcgcacgcgt cgagatcgac ctgccgctct
     7921 tcgcgtccgc cgacagcttc gcgggcgtgc cgctgccgct cggggtgcac ctgcccttcg
     7981 ccttcgtcgc caagggacgc atgtgccgca ccgcaggtct cgcgcgcgag ggcccggagc
     8041 ggttcgcggc gcggcccttc tgccaccacg aatgcctcgg actcgccgcc cgcaccgagc
     8101 gcacacgcga cggtacacac gacgcctggg agaccctgca cgtgggcaac accgtgttct
     8161 gccgccactc cgccgccaac ctcgccacgc tcgccagcgc tgtcgcggcg ggccgggtgg
     8221 cgcgcctgat cgtgcccggc gagaccttgt gaggaaacgc gcatgaagat cgtcgccccc
     8281 atccggcaac tcgacgagat cgccgcgctg gcgcgcgccg gggcggacga actctattgc
     8341 ggggtgacgc cgcgcgagtg ggccgagcgc ttcggtggcg ccagcgccaa ccgccgtccc
     8401 ggcggcaacc tcccctcgct ggccgcgctc gccgaggcgg tggcgctcgc ccaccgcaat
     8461 ggcgtacagc tctccctggt gctcaacgca cagcagtatt cggtcgaaca gatcgagttc
     8521 gcgctcgcga tcgcgcaccg ctacgtcgac atggggggcg atgcggtgat cgcgagcgac
     8581 cccggcctgc tcctggcgct ggccgaagcc gagccggagc tgcgcatcca cgtcagctcg
     8641 gtggccacct gccgcaatgc cgacggcgcc cgcctctacc gcgagctcgg cgcgcgccgg
     8701 ctgatcctgc cgcgcgacat caccctcgac gaggccgcgg agatcgccgc cgcagtgccc
     8761 gacctggaga tcgaggcctt cgtgctcaac gacggctgcg tctacgagga aggcagctgc
     8821 aacaccctgc acctcccggg cgcgctcggc gggccgatct gcctggaccg ctacgcctac
     8881 gcgcaccgcc accgcgacgg ccggccgctc tcggccgcgc tcgcggcccg cctgcaggag
     8941 aacgacgagg cctatcggcg ctggctgtgg taccgcttct cctgcggctt caccaccacc
     9001 gccgacggcc tgcccttcgg cccctgcggc ctgtgcgcga tcccggcgtt cgggcgcggc
     9061 ggcatccacg cgctcaagat cgccggccgc gagggtccgc ccgagcgcaa gctcgccagc
     9121 gtgcgcatgg tccggcggat cctcgacgcc cacgacaacg gcgaagcccc cgcggcggtg
     9181 atggcccgtg cgcgcaacct gcggcctgcg cacgaacact gcgcgaccgg cttcatgtgc
     9241 tactacccgg aggtcgtctc ccgcgcatcc gaagcggcgc agccgctgtg cgacggtcgc
     9301 gcagcaggtg ctcagtagat cggctccacg agcaggcggt cgtccacata gttctgcagc
     9361 aggttgttga tcgcgaacag cgtcccggtc gaggacgacg gacaacctcc gcaggcgccc
     9421 ttgtagcgca ccgtcagggt gaattccttg agcttgacca cgtcgaggcc gccgccgtcc
     9481 tggtcgagga agggccggat gtgctcgtcg aggatctggt tgatgcgcat cagcagttcg
     9541 gcatcgtcgg cctcgacctc caccggctcc tcggccacgg cctcgcctgc gccgatcgcg
     9601 gcgcccgccg ccccacccgg tcctgccggg ttggcgatgc cgatgacgcg gcgcgccttg
     9661 tgcccctcga tcagctcggt gaccgcgggc tcgatcgccg accagggcgt gcccgggtcg
     9721 cgggtgatgg tgatgaagcc gccggtgcaa tagatgtcga cgacgccggg caccgccagc
     9781 aggctctcgc ccagggtgtc gccgacgacc gcgacaccgg cgctgaaatg acgcggcgcg
     9841 cccgtataca gcggcgcatc gagcatgaac ttgttcgcca gcgggttggg cgtggacatg
     9901 acggcggtga cattgggcat ggcggactcc tccggcttgg tagggctgtg ctgcgactgg
     9961 gggaagcgat ccgtgagcac caggacaagg cggagtgcgg gacggattgc gacactgcgg
    10021 aacgaattct acttgtcgtt aacgtcaacg tcaattaaga tgaacctgca tgccactcga
    10081 accgcttgca ggaacctgat cccgacatga aggaatatct gctgctactg ctcggcacgg
    10141 ccctggtgaa caacgtgatc ctggtccgct tcctcggcct gtgccccttc ctcggggtct
    10201 cgggcaagct cgagaccgcg ctcggcatgg ggctcgccac caccttcgtg ctcaccctca
    10261 cctcgggcgc ggcgtgggtg gtcgagcacg ccctgctgca gccgctcgac atgctgcacc
    10321 tgcgcatcct gacctacatc ctggtgatcg ccgcggtggt gcagttcacc gagatggcga
    10381 tccagaaggc cagtccggtg ctgcaccaca ccctgggcat ctacctgccg ctgatcacca
    10441 ccaactgcgc ggtgctcggc acggcgctgc tcaacaccca gcaaggcaac ggcctggtcg
    10501 caagcatgct ctatggcgcc ggctcggcgc tcggcttcac gctggtgatc ctcctcttcg
    10561 ccggcctgcg cgagcgcatc gcgctcgcga acgtgcccgc cgccttcgcc ggcgcgccga
    10621 tcgcgctgat caccgccggc ctgctgtcgc tggccttcat gggtttcgcc ggcctgccgg
    10681 tgcgctgaaa cctccccacg aacgcgcaag gagcacgacg atgacgatga ttctctcggt
    10741 agtggcggtc gccgccctgg gcctcgccct gggctggctg ctcggtctcg cctcgcagtg
    10801 gctgggagcg accaccgacc cgggggtgga gcgcatcgcc gaggccctgc ccggttcgca
    10861 atgcggccag tgcggcttcg cc
//
LOCUS       DAOQNN010000064        10816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2837_length_10816_cov_11.231763, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000064 DAOQNN010000000
VERSION     DAOQNN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10816
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2837_length_10816_cov_11.231763"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1145)
                     /locus_tag="PKV97_05615"
     CDS             complement(120..1145)
                     /locus_tag="PKV97_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB05391.1"
                     /translation="MNERQHAPAVTVSIRMVCQILDRLGEHRPLALECVERAGIASAL
                     LEHESARVTVEQFALFYRTLAVELDDETPGLFSRPLRPGTLKFLCLGMLDAANLRVAL
                     HRFCWFFRLVLDDLHFELGEDEGLSRIALVERVALQPHRPLILELMLMLVQGIASWMI
                     ERKLLFARVDLAYPAPPHAGEYINMFAGPACFDRPLTALYIEPAFMDAPIRQDKAALS
                     AFLRKAPMDWIHVSVSERLFTHRVRDLLEAGLGSPQSVEDVARMLHISARTLARRLDA
                     EGTHFQAVKDALRRDVAIARISRTDEPIGSIGADLGFDDPAAFNRAFKQWTGSPPGSY
                     RRAGAGR"
     gene            1363..2196
                     /locus_tag="PKV97_05620"
     CDS             1363..2196
                     /locus_tag="PKV97_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB05392.1"
                     /translation="MHPGKASPILEVEGLTLSFGGIKALTDVGFEVAPGSITTVIGPN
                     GAGKTSLFNTISGFYKPSVGRIRFQGADITATRPPERARLGLARSFQNIALFRGMTVL
                     DNIKLGRHAHLRTNVFDALVYFGRARHEEMKLREEIEERIIDFLEIDHIRHMPVSVLS
                     YGLQKRVEMARALAMRPKVLMLDEPVAGMNREETEDMARFILDVREEWGVTVLMVEHD
                     MGMVMDLSDHVVVLNFGQVIADGTPAEVQADPEVIKAYLGSGEVSELRQRLHQALAVE
                     A"
     gene            2198..3088
                     /locus_tag="PKV97_05625"
     CDS             2198..3088
                     /locus_tag="PKV97_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB05393.1"
                     /translation="MSLEFDWLFLAEIVLAGLGAGGLYALTGLAFVMIYKATRVVNLA
                     IGEMLMIGAYLCFGLATAMNLPMWLAILVAVIGSGGAGALLERVAIRPMLGESPIAVF
                     MVTVGLASILVGVVELIWTSEPRRLPEFMPATPVMIGEAFVPPKVFYGFLVAAVLIGV
                     ALAVFRFWRGGVALRATASDQQAAYSMGIDVPKVFSLSWTVAAMIAAIAGVIVGAVGG
                     ISSTMGVFGLSVLVVVIVGGLDSVLGALVAGLLIGVVESLAGGFLGGEYKLLATFLIL
                     IVVLLVRPYGLFGTHEIERL"
     gene            3088..4128
                     /locus_tag="PKV97_05630"
     CDS             3088..4128
                     /locus_tag="PKV97_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB05394.1"
                     /translation="MRIGTARSSYRHDEALFDSRTQHAWLAILAVALIGFPFLADEYW
                     LYLACLVAINIASTTGLNILTGYTGLVSLGQAAFMGIGAYTVAALEQKLGAPVLLNLL
                     AGGFVAMLGGLVVGLPSLRVKGLYLAIATIAASVVLHFVFANWHAVTGGHAGITLVPA
                     EILGLSFDTSFRLYWLFVPITILMVAGAANLFRTRIGRAFIAIRDRDISAEVLGIPLL
                     RYKLLSFGLSSFYAGVAGGMWAYFFRVVTPESFPLILSIFFLAAVIVGGMGTILGAIL
                     GAVFMTMVPEALKLVVGWLPLSADATLILSPVRTIVFGVLIVGFLVFEPLGLQEIWRR
                     IRRFFHLWPFRA"
     gene            4178..5347
                     /locus_tag="PKV97_05635"
     CDS             4178..5347
                     /locus_tag="PKV97_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB05395.1"
                     /translation="MRRTVLKSLLATAILSATAGLPVARAADKEIVIGGSIPMSGVFA
                     FAGIGIHAGIQDYVKMVNAAGGIAGHTLRYVPEDSAYKVDQSVAAFKKITSQNKVNFY
                     YGDSTAFSKTINPELDRAGSMIMSGASFATELNDPKKYPYQFVAGPDYTQQFGILLRY
                     IAQQKPGAKVAFVHSDTEFGRDPIETSRGVAKALGLDVAIEIMTPPGAVDVSTEIAKL
                     RRARPDFTIFHGYILGPIPEFIGQARQMGLDTQFMGTFWTMDNSTVMQMGELAEGFMG
                     VMPYNYYYDQSAPAPMLDKIRAMRPEYQNVPYIQGFLSAMLYAESARRTLAAGKEMSA
                     ANLKAALNSIENFDTGGLIGVPVTLRGNTIPVGRIYRADTKARRMVPASDWIVLE"
     gene            5351..6163
                     /locus_tag="PKV97_05640"
     CDS             5351..6163
                     /locus_tag="PKV97_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB05396.1"
                     /translation="MSAAESVLEVNNIEVIYNKAVQVLRGLSLAVPRGQIVALLGSNG
                     AGKSTTLKTVSGLLGLENGELTRGEIRFEGRPLADSKPHELVRAGLFHVMEGRRVFED
                     LSVEENLVAATYALTGRKGEKADKELVYEYFPRLYERRNGLAGYLSGGEQQMLAIGRA
                     LIARPRLILLDEPSLGLAPLLVENIFRIIARINAEQKVSMLLVEQNASVALAVAHYGY
                     IMENGKVVTDGPAERLAGDQDVREFYLGMGGHGEMKSFRDIKHYKRRKRWLS"
     gene            6178..8007
                     /locus_tag="PKV97_05645"
     CDS             6178..8007
                     /locus_tag="PKV97_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB05397.1"
                     /translation="MSNNLPTLTLPQMLREQARLQPGRPAIRQKDFGIWQAIGWDDYY
                     RRASHVGLGLRALGLPAGGHVGVLAENRIEWVLAQMGCGLVGAIAVGVYPTSPAPEVA
                     YVLNHADVEVVVCEDQEQTDKVIEALDQLPHLRAIVVMETKGLRNADERVRDRILSFA
                     DLERLGVEYEATHRHLVEETLAAQRLDDLALMIYTSGSTGKPKGAMIRYANIRGVAPG
                     IIDRLGLAADTVHLSYLPLCHVAEQMLTTFCPIYLGSTVAFGESIRTVQEDLREIAPT
                     MFLGVPRIWEKLHSAITIRMQESGPVQRWLFARALAACAPFAYKSAAQRTAGERLTHA
                     ICYGLILRALQNFIGLRKARVALTGAAPIPPAVVHFFRTLGVPLVEVYGLTESTGMIT
                     GQPLDAVAFGSVGTPILGVEHRIGARGELLVHGEMVFAGYYKNPEATADAIRDGWLHT
                     GDVVEERDGQLYIVDRLKDIMITAGGKNLTPSEIEKAMKSSPYIKECIVVADGRKFVS
                     ALIQIDYETVSRWAEGRQIPFTHFRSLVEHPEVRALIEAEIARGNAQLAQVSHIRRFH
                     LLNKELDHDDGEVTATMKVRRASVYKTYADVIEAMYRGGAAHA"
     gene            8000..8815
                     /locus_tag="PKV97_05650"
     CDS             8000..8815
                     /locus_tag="PKV97_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB05398.1"
                     /translation="MPDPIHGDCVRLEREGGLAFITLDRPQARNALDLAMGLGLRDAV
                     RELAAAPPRVLVLRSSGAHFMVGGDVRRFDALLGESQAACTDEIGRLIDAVHEAVTGL
                     TRLPCPVLGLVGGSVAGFGLALAMACDLLVAADDARLVLAYGAIGATPDGGASWQLPR
                     RVGLQRALAIALLDPQLDAVQAREIGLVAEVVPAAQLAEAGLALARRLAAGPAAAQAG
                     IKRLLRAGLDTDLATALDAEKASFLALSATPDFAEGVRAFCERRPARFARPSG"
     gene            8934..10070
                     /locus_tag="PKV97_05655"
     CDS             8934..10070
                     /locus_tag="PKV97_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB05399.1"
                     /translation="MDFQLNPEQREIRDLARRFAEREIAPRAAQADLDKAFPTEVHQR
                     AQELGLLGINLPETVGGGGLGCLELVLVTEAFCRGCLGIGTALCVNALATEPIVIAGT
                     DAQRSRYLGAAARGALASFAMTEPGAGSDVAGIRTRAERVEGGYRLTGSKIWISNANL
                     AEFFVVFAKTAPDGGHRGMSAFIVPRASAGLTVGEPLGKLGQRCSPTCEVFLDQVFVP
                     EDHRLGEEGGGFALAMAVFDHSRPMVAAFGVGLIERCLDESLAYATQRSSMGKRLIDH
                     QAVAHKLAEMRMRLEAARLLTYQSAWLLDQGRPNTIEASIAKAFAADAAMWAATEAVQ
                     IFGGMGYSTEYPVEKLFRDAKVLQIYEGTSEIQRNIIARELQRA"
     gene            10087..>10816
                     /locus_tag="PKV97_05660"
     CDS             10087..>10816
                     /locus_tag="PKV97_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HNB05400.1"
                     /translation="MNPSNPSNPNEALILGAVRTPFGRRGGALRETRPDELLATAIGG
                     VLARTGLPGDKVGDVLAGCVSQAGEQGANIARQALLIAGLPAEVPGVSMNRMCGSSQY
                     ATHAAAQAVAAGDLDFAIGCGVESMSRVPMFLDLTLGRGDFRGFDNLHPKILERFAIP
                     HQVESAERIAGQWKISRTEMDDYAIESHRRAEAARAAGVHSEILPVAGVDKEGGAITL
                     AHDEGIRAVIDVERMRAMQPVFRQP"
BASE COUNT         1657 a         3861 c         3626 g         1672 t
ORIGIN      
        1 tggggcgtcg tgccgtctac gcggaacgca gagcttgagc ccccgcggat ccgtccccgg
       61 gtacgcgcgc cgcaaggcgc gaacgcggcc gccgtccgtt cggcgaccac gtcacaggct
      121 cagcggcccg cccccgccct gcggtagctg cctggcgggg atcccgtcca ttgcttgaag
      181 gcgcgattga aagccgcggg atcgtcgaag ccgagatcgg cgccgatgct gccgatcggc
      241 tcgtcggtgc gcgagatccg cgcgatggcg acgtcgcggc gcagcgcgtc cttgaccgcc
      301 tggaagtgcg ttccttccgc atcgagccgg cgggcgagcg tgcgcgccga gatgtgcagc
      361 atacgggcaa cgtcttccac ggactgcggg cttcccagcc cggcttcgag cagatcgcgc
      421 acgcggtgcg tgaacagccg ctcgctcacg gagacgtgga tccagtccat cggcgccttg
      481 cgcaggaagg ccgacagcgc ggccttgtcc tggcggatcg gggcgtccat gaaggccggc
      541 tcgatgtaga gcgcggtgag cgggcgatcg aagcaggccg ggccggcgaa catgttgatg
      601 tactcacccg catgcggcgg cgccggatag gcgagatcca cgcgcgcgaa caggagcttg
      661 cgctcgatca tccacgaagc gatgccctgc accagcatca gcatcaattc gaggatcagg
      721 gggcggtggg gttggagcgc aacgcgctcg acgagcgcga tgcggctcag gccctcgtcc
      781 tcacccagct cgaagtgcag atcgtccagc accaggcgga agaaccagca gaagcggtgc
      841 agcgcgacgc gcaggttggc ggcgtcgagc atgccgagac agaggaactt cagcgtgcca
      901 gggcgcagcg ggcgtgagaa cagccctggc gtctcgtcgt cgagttcgac cgcgagggtg
      961 cggtagaaga gcgcgaactg ctcgaccgtc acacgggccg attcgtgttc cagcagcgcg
     1021 gaagcgatcc cggcgcgctc gacgcattcc agggccagcg gcctgtgctc acccagccga
     1081 tccaggatct ggcagaccat gcggatggag acggtgaccg ccggtgcgtg ctgacgctcg
     1141 ttcatgtgca agagattggt ggcagaaaat gtcaatcgat cggccggttc tgtcatccac
     1201 aaggtcggcc atactgccta gtatggcgtg caaatagctg cagcgaaaag ttttatgtct
     1261 gcgtcggcgg ttcgaggcct ctcgtgtggt cgcgctcgct ggcaattaaa gcacagcacg
     1321 cacaggtcac ccgcaaagag gtggccggca ggaggagaca aggtgcatcc aggcaaggcc
     1381 agtccgatcc tcgaggtcga ggggctcaca ttgtcgtttg gcggcatcaa ggcgctgacc
     1441 gacgtgggct tcgaagtcgc tccggggtcc atcaccaccg tcatcggtcc gaacggcgcg
     1501 ggtaagacct cgctgttcaa cacgatctcc ggtttctaca agccctcggt ggggcgcatc
     1561 cgcttccagg gcgcggacat caccgccacc cgcccgcccg agcgcgcccg gctgggcctg
     1621 gcgcgcagct tccagaacat cgcgctgttc cgcggcatga ccgtgctcga caacatcaag
     1681 ctcggtcgtc acgcccactt gcgcaccaac gtcttcgatg cgctggtcta tttcggccgc
     1741 gcccggcacg aagagatgaa gctgcgcgag gagatcgagg aacgcatcat cgacttcctc
     1801 gagatcgacc acatccgcca catgccggtg tcggtcctct cctacgggct gcagaagcgg
     1861 gtggagatgg cgcgcgcgct ggcgatgcga cccaaggtgc tgatgctcga cgagcccgtg
     1921 gcgggcatga accgcgagga gaccgaggac atggcgcgct tcatcctcga cgtgcgcgag
     1981 gagtggggcg tgacggtact gatggtcgag cacgacatgg gcatggtcat ggacctctcc
     2041 gaccacgtcg tggtgctcaa cttcggccag gtcatcgccg acggcacgcc ggccgaggtg
     2101 caggccgacc ccgaggtgat caaggcctac ctgggctcgg gcgaggtctc cgagctgcgt
     2161 cagcgcctcc accaagcgct tgccgtggag gcctgagatg agcctggaat tcgactggct
     2221 gttcctcgcc gagatcgtgc tcgccggcct cggtgccggc ggcctgtacg cgctgaccgg
     2281 cctcgccttc gtgatgatct acaaggccac gcgggtggtg aacctggcca tcggcgagat
     2341 gctgatgatc ggcgcctacc tgtgcttcgg cctggccacc gccatgaacc tgccgatgtg
     2401 gctggcgatc ctggtcgcag tgatcggcag cggcggggcc ggcgcactgc tcgagcgcgt
     2461 ggcgatccgc cccatgctgg gcgagtcgcc gatcgcggtc ttcatggtca ccgtgggcct
     2521 ggcctccatc ctggtcggcg tcgtcgagct gatctggacc tcggagccgc gccgcctgcc
     2581 ggagttcatg cccgcgacgc cggtgatgat cggcgaggcc ttcgtgccgc ccaaggtgtt
     2641 ctacggcttc ctcgtcgcgg ccgtgctgat cggcgtggcg ctggcggtgt tccgcttctg
     2701 gcgcggcggc gtggcgctgc gggcgacggc ctcggaccag caggcggcct actcgatggg
     2761 catcgacgtg cccaaggtgt tctcgctgtc ctggacggtg gcggcgatga tcgcggcgat
     2821 cgccggcgtc atcgtcggcg cggtgggcgg catctcctcg acgatggggg tgttcggcct
     2881 gtcggtgctg gtggtggtga tcgtcggcgg cctcgacagc gtgctcggcg cgctggtcgc
     2941 cggcctcctc atcggtgtcg tcgagtcgct cgcgggcggc ttcctcggcg gtgaatacaa
     3001 gctgctggcc accttcctga tcctgatcgt cgtgctgctg gtccgtccct acggactgtt
     3061 cggcacccac gaaatcgaga ggctgtaatg cgcatcggaa ccgcacgctc cagctaccgc
     3121 cacgacgagg cgctgttcga cagccgcacc cagcacgcct ggctggcgat cctcgccgtc
     3181 gcgctgatcg gcttcccctt cctcgccgac gagtactggc tctacctcgc ctgcctggtg
     3241 gcgatcaaca tcgccagcac caccgggctc aacatcctca ccggctacac cgggctggtg
     3301 agcctgggcc aggccgcctt catgggcatc ggcgcctaca ccgtcgccgc gctggagcag
     3361 aagctgggcg cgccggtgct cctcaacctg ctcgccggcg gcttcgtcgc catgctcggc
     3421 gggctggtgg tgggcctgcc ctcgctgcgg gtcaagggcc tgtacctggc gatcgcgacc
     3481 atcgccgcct cggtggtgct gcacttcgtg ttcgccaact ggcatgcggt caccggcggc
     3541 catgcgggca tcaccctggt gccggcggag atcctcggcc tcagcttcga cacctccttc
     3601 cgcctgtact ggctgttcgt gccgatcacc atcctgatgg tggccggcgc ggccaacctc
     3661 ttccgcaccc gcatcggccg cgccttcatc gccatccgcg accgcgacat ctccgccgag
     3721 gtgctgggca ttccgctgct gcgctacaag ctgctgtcct tcggtctgtc gtccttctac
     3781 gccggggtgg cgggcgggat gtgggcctac ttcttccgcg tggtcacgcc ggagagcttc
     3841 ccgctgatcc tgtcgatctt cttcctcgcc gcggtcatcg tcggcggcat gggcaccatc
     3901 ctcggcgcca tcctcggcgc ggtcttcatg accatggtgc ccgaggcgct gaagctcgtc
     3961 gtcggctggc tgccgctgtc ggccgacgcc acgctgatcc tgtcgccggt gcgcaccatc
     4021 gtgttcggcg tgctgatcgt cggcttcctg gtgttcgagc cgctcggcct gcaggagatc
     4081 tggcgccgca tccgccgctt cttccacctc tggccgttcc gcgcctgagc tttcctcgcc
     4141 gcaccgcttc accccatatc aagagaagga gacaagcatg agaagaaccg tactcaagag
     4201 cctgctggcc accgcgatcc tctccgccac cgccggcctg cccgtagccc gggcggccga
     4261 caaggagatc gtcatcggcg gctccatccc gatgagcggc gtgttcgcct tcgccggcat
     4321 cggcatccac gccggcatcc aggattacgt gaagatggtc aacgccgccg gcggcatcgc
     4381 aggccacacg ctgcgctacg tgcccgagga cagcgcctac aaggtcgacc agtcggtggc
     4441 ggcgttcaag aagatcacca gccagaacaa ggtgaacttc tactacggcg actccaccgc
     4501 cttcagcaag accatcaacc ccgagctcga ccgcgccggc tcgatgatca tgtcgggcgc
     4561 ctccttcgcc accgagctca acgacccgaa gaagtaccct taccagttcg tcgccggccc
     4621 ggactacacc cagcagttcg gcatcctgct gcgctacatc gcccagcaga agcccggcgc
     4681 caaggtggcc ttcgtccatt ccgacaccga gttcggccgc gacccgatcg agaccagccg
     4741 cggcgtggcc aaggcactcg ggctcgacgt cgccatcgag atcatgaccc cgccgggcgc
     4801 ggtcgatgtg tccaccgaga tcgccaagct gcgccgcgcg cggcccgact tcaccatctt
     4861 ccacggctac atcctcggcc ccatccccga attcatcggc caggcgcgcc agatgggcct
     4921 ggacacccag ttcatgggca ccttctggac catggacaac tcgaccgtga tgcagatggg
     4981 cgagctcgcc gaaggcttca tgggcgtgat gccctacaac tactactacg accagagcgc
     5041 accggcgccg atgctcgaca agatccgcgc gatgcgtccc gagtaccaga acgtgcccta
     5101 catccagggc ttcctgtcgg ccatgctgta cgccgagtcg gcccgccgca cgctggccgc
     5161 aggcaaggag atgagcgcgg ccaacctcaa ggcggcgctc aactcgatcg agaatttcga
     5221 caccggcggc ctgatcggcg tgcccgtcac cctccgcggc aacaccatcc cggtcggccg
     5281 catctaccgc gccgacacca aggccaggcg catggtgccg gcctccgact ggatcgtgct
     5341 ggagtaagcg atgagtgcgg ccgaatccgt cctcgaggtg aacaacatcg aggtgatcta
     5401 caacaaggcg gtgcaggtgc tgcgcggcct ctcgctggcg gtgccgcgcg ggcagatcgt
     5461 cgccctgctc ggctccaacg gcgccggcaa gagcaccacg ctcaagaccg tctccggcct
     5521 cctcgggctg gagaacggcg agctgacgcg cggcgagatc cgcttcgagg gcaggccgct
     5581 ggcggattcc aagccgcacg agctggtgcg cgcggggctc ttccacgtga tggaggggcg
     5641 ccgcgtgttc gaggacctca gcgtcgagga gaacctcgtc gccgccacct acgcactcac
     5701 cgggcgcaag ggcgagaagg ccgacaagga gctcgtctat gaatacttcc cgcgcctgta
     5761 cgagcgccgc aacggcctcg ccggctatct gtcgggcggc gagcagcaga tgctggcgat
     5821 cggccgcgcg ctgatcgccc gccccaggct gatcctgctc gacgagccct cgctgggcct
     5881 cgcgccgctg ctggtggaga acatcttccg catcatcgcc cgcatcaacg ccgagcagaa
     5941 ggtgtcgatg ctgctggtgg agcagaacgc ctcggtggcg ctggcggtgg cgcactacgg
     6001 ctacatcatg gagaacggca aggtcgtcac cgacggcccg gccgagcgtc tcgccggcga
     6061 ccaggacgtg cgcgagttct acctcggcat gggtgggcac ggcgagatga agagcttccg
     6121 cgacatcaag cactacaagc gccgcaagcg ctggctgtcg tgatcgagag ggtggccatg
     6181 agcaacaacc tacccacgct gaccctgccg cagatgctgc gcgagcaggc gaggctgcaa
     6241 cccgggcggc ccgccatccg ccagaaggac ttcggcatct ggcaggccat cggctgggac
     6301 gactattacc gccgcgcgag ccatgtcggc ctcggcctgc gcgcgctcgg cctgcccgcc
     6361 ggcggccatg tcggcgtgct cgccgagaac cgcatcgaat gggtgctggc gcagatgggc
     6421 tgcggcctcg tcggcgccat cgcggtcggc gtctatccga ccagcccggc acccgaggtg
     6481 gcctacgtgc tcaatcacgc cgacgtcgag gtcgtggtgt gcgaggacca ggagcagacc
     6541 gacaaggtga tcgaggcgct cgaccagctg ccgcacctgc gcgccatcgt ggtgatggag
     6601 accaagggcc tgcgcaacgc cgacgagcgc gtgcgcgatc gcatcctgtc cttcgccgac
     6661 ctggagcggc tcggcgtcga atacgaggcc acccaccgcc acctggtcga ggagaccctg
     6721 gccgcgcagc ggctcgacga cctcgcgctg atgatctaca cctcgggctc caccggcaag
     6781 cccaagggcg cgatgatccg ctacgccaac atccgcggcg tcgcacccgg catcatcgac
     6841 cgcctcgggc tcgcggccga caccgtgcac ctgtcctacc tgcccctttg ccacgtcgcc
     6901 gagcagatgc tgaccacctt ctgcccgatc tacctcggct ccaccgtcgc cttcggcgag
     6961 tcgatccgca ccgtgcagga ggatctgcgc gagatcgcgc ccacgatgtt cctcggcgtg
     7021 ccgcgcatct gggagaagct gcactcggcg atcacgatcc ggatgcagga gagcgggccc
     7081 gtgcagcgct ggctgttcgc acgcgcgctg gcggcctgcg cgcccttcgc ctacaagtcg
     7141 gcggcgcagc gcacggcggg cgagcgcctg acccacgcca tctgctacgg cctcatcctg
     7201 cgcgcgctgc agaacttcat cggcctgcgc aaggcgcgcg tggcgctgac cggcgcggcg
     7261 ccgatcccgc ccgcggtggt gcacttcttc cgtaccctcg gggtgccgct ggtcgaggtc
     7321 tacggcctca ccgaatcgac cggcatgatc accggccagc cgctcgacgc ggtggccttc
     7381 ggttcggtcg gcacgcccat cctcggcgtc gagcaccgca tcggcgcgcg tggcgagctg
     7441 ctggtccacg gcgagatggt cttcgccggc tactacaaga acccggaggc gaccgccgac
     7501 gccatccgcg acggctggct gcacaccggc gacgtggtcg aggagcgcga cggccagctc
     7561 tacatcgtcg atcgcctcaa ggacatcatg atcaccgccg gcggcaagaa cctgacgccg
     7621 tccgagatcg agaaggcgat gaagtccagc ccctacatca aggagtgcat cgtcgttgcc
     7681 gacgggcgca agttcgtctc ggcgctgatc cagatcgact acgagacggt gtcgagatgg
     7741 gccgagggcc gccagatccc cttcacccac ttccgctcgc tggtcgagca ccccgaggtg
     7801 cgcgcgctga tcgaggccga gatcgcgcgc ggcaacgccc agctcgccca ggtctcgcac
     7861 atccgccgct tccacctgct gaacaaggag ctcgaccacg acgacggcga ggtcacggcg
     7921 accatgaagg tgcgtcgcgc gagcgtctac aagacctacg ccgacgtgat cgaagccatg
     7981 taccggggag gggccgccca tgcctgaccc gatccacggc gactgcgtgc gactcgagcg
     8041 tgaaggcggg ctcgccttca tcaccctcga ccgcccgcag gcgcgcaacg ccctcgacct
     8101 ggcgatgggg ctggggctgc gcgacgccgt gcgcgaactt gccgccgcgc cgccgcgcgt
     8161 gctcgtgctg cgcagcagcg gcgcgcactt catggtcggc ggcgacgtgc gccgcttcga
     8221 cgccctgctc ggcgagtcgc aggccgcctg taccgacgag atcggccgcc tcatcgatgc
     8281 ggtgcacgag gccgtcaccg gcctcacccg gctgccttgc ccggtgctgg gtctggtggg
     8341 cggctcggtg gcgggcttcg gcctggcgct ggccatggct tgcgacctgc tggtcgcggc
     8401 cgacgacgcg cgcctcgtgc tcgcctacgg tgccatcggc gccacgcccg acggcggcgc
     8461 gagctggcag ctgccgcgcc gcgtcggcct gcagcgggcg ctcgcgatcg cgctgctcga
     8521 cccgcagctc gatgcggtgc aggcgaggga gatcggcctg gtggccgagg tggtgccggc
     8581 ggcgcagctg gccgaggccg ggctggcgct ggcccgacgc ctggccgcgg gaccggccgc
     8641 cgcccaggcc ggcatcaagc gcctgctgcg cgcgggcctc gacaccgacc tggcgacggc
     8701 gctcgacgcc gagaaggcga gcttcctcgc cctctccgcg acgccggact tcgccgaggg
     8761 cgtgcgcgcc ttctgcgagc ggcggccggc gcgcttcgcg cgaccctccg gataggctcg
     8821 cccgcgacgc gctgcccgag cgccttccac ccgcaatccc cgtcggcgtg cccaggcatg
     8881 cccgcccctt tattgcccgt cgtctgcgca cggcggcttc gcatggagac accatggact
     8941 tccagttgaa cccggagcag cgcgagatcc gcgacctggc gcgccgcttc gccgagcgcg
     9001 agatcgcccc ccgcgccgcg caggccgatc tcgacaaggc tttcccgacc gaggtgcacc
     9061 agcgcgcgca ggagctcggc ctgctcggca tcaacctgcc cgagacggtc ggcggcggcg
     9121 gcctcggctg cctggagctg gtgctggtca ccgaggcttt ctgccgcggc tgcctgggca
     9181 tcggcaccgc gctgtgcgtg aacgcgctcg ccaccgagcc gatcgtcatc gccggaaccg
     9241 atgcgcagcg cagccgctac ctcggcgccg cggcccgcgg tgcgctcgcc agcttcgcga
     9301 tgaccgagcc cggcgccggc tccgacgtgg ccggcatccg cacccgcgcc gagcgcgtcg
     9361 agggcggcta tcgcctcacc ggcagcaaga tctggatctc caacgccaac ctcgccgagt
     9421 tcttcgtcgt cttcgccaag accgcgccgg acggcggcca ccgcggcatg agcgccttca
     9481 tcgtgccgcg cgccagcgcc ggcctgacgg tgggcgagcc cttgggcaag ctcggccagc
     9541 gctgttcgcc cacctgcgag gtcttcctcg accaggtctt cgtgcccgag gaccaccgcc
     9601 tcggcgagga gggcggcggc ttcgcgctgg cgatggcggt gttcgaccac tcgcgcccga
     9661 tggtggccgc cttcggcgtc ggcctcatcg agcgctgcct cgacgagtcg ctcgcctacg
     9721 ccacccagcg cagcagcatg ggcaagcgcc tgatcgacca ccaggccgtg gcccacaagc
     9781 tcgccgagat gcgcatgagg ctcgaagccg cgcgcctgct cacctaccag tcggcctggc
     9841 tgctcgacca gggtcggccg aacaccatcg aagcctcgat cgccaaggcc ttcgccgcgg
     9901 acgcggcgat gtgggcggcg accgaggcgg tgcagatctt cggcggcatg ggctactcga
     9961 ccgagtaccc ggtggagaag ctcttccgcg acgccaaggt gctgcagatc tacgaaggca
    10021 ccagcgagat ccagcgcaac atcatcgccc gcgaactgca gcgggcctga acccgaggac
    10081 ccggaaatga acccaagcaa tccatcgaac ccgaacgagg cgctgatcct gggcgccgtg
    10141 cgcaccccct tcggccgccg cggcggcgcc ctgcgcgaga cccgcccgga cgagctcctc
    10201 gccaccgcca tcggcggcgt gctggcgcgc accggcctgc ctggcgacaa ggtcggcgac
    10261 gtgctcgccg gctgcgtcag ccaggccgga gagcagggcg ccaacatcgc ccgccaggcg
    10321 ctgctgatcg ccggcctgcc cgccgaggtg ccgggcgtgt cgatgaaccg catgtgcggc
    10381 tccagccagt acgccaccca cgccgcggcg caggcggtgg ccgcgggcga cctcgacttc
    10441 gccatcggct gcggcgtcga gagcatgagc cgggtgccga tgttcctcga cctgaccctg
    10501 ggccggggcg acttccgcgg cttcgacaac ttgcacccga agatcctcga acgcttcgcc
    10561 atcccgcacc aggtcgagag cgccgagcgc atcgccgggc agtggaagat ctcgcgcacc
    10621 gaaatggacg actacgcgat cgagagccac cgccgcgccg aggccgcgcg tgcggccggc
    10681 gtgcacagcg agatcctgcc ggtggcgggg gtggacaagg agggcggggc gatcacgctc
    10741 gcgcacgacg agggcatccg cgcggtcatc gacgtcgagc gcatgcgcgc catgcagccg
    10801 gtgttccgcc agcccg
//
LOCUS       DAOQNN010000065        10650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2910_length_10650_cov_8.743275, whole genome shotgun sequence.
ACCESSION   DAOQNN010000065 DAOQNN010000000
VERSION     DAOQNN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2910_length_10650_cov_8.743275"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PKV97_05665"
     CDS             complement(<1..166)
                     /locus_tag="PKV97_05665"
                     /EC_number="5.4.2.2"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924611.1"
                     /note="catalyzes the interconversion of alpha-D-mannose
                     1-phosphate to alpha-D-mannose 6-phosphate and
                     alpha-D-glucose 1-phosphate to alpha-D-glucose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNB05401.1"
                     /translation="MRHPLPAAELFKAYDIRGIVDTVLTPTAVRAIGHAIGSEAAVRG
                     QRAIAVGRDGR"
     gene            complement(301..729)
                     /locus_tag="PKV97_05670"
     CDS             complement(301..729)
                     /locus_tag="PKV97_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB05402.1"
                     /translation="MSRPVFTTALEAEAAFYRALSEGDFEALMAVWSEEEEVVCVQPG
                     GPRIVGLAAVREIWRQILADGARIRIDISHAVTSSTAMMAMHCVLERLATDGPTPRTA
                     TIAATNVFVRGADGWRMVLHHASPVPEISGPEDSTPRVLH"
     gene            complement(829..1029)
                     /locus_tag="PKV97_05675"
     CDS             complement(829..1029)
                     /locus_tag="PKV97_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HNB05403.1"
                     /translation="MVENTDKTQAVVTTAKDLPLHCPPPGAPLWARHPRVFLDILKTG
                     EAVCPYCSAHYVFNGERPKGHH"
     gene            complement(1054..1974)
                     /locus_tag="PKV97_05680"
     CDS             complement(1054..1974)
                     /locus_tag="PKV97_05680"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNB05404.1"
                     /translation="MSMADRDGFIWQDGKLVPWREATTHVLTHSLHYGLAVFEGVRAY
                     NTVSGTAIFRLAEHTQRLINSGKIYMMDIPYSKDELMEAQKEVVRANKLESCYLRPIA
                     FYGSEKMGISTLGARVHVAIAAWPWGAYLGEEGLQKGIRVKTSSYTRHHVNSTMPRAK
                     LSATYPNSILANLEVTRMGYDEALLLDNQGFVAEGAGENLFIVKDGRIYEPEIASALT
                     GITRDSIHVIARELGYEVGTKRLTRDDIYLADEAFFTGTAAEVTPIRELDDRQIGEGR
                     RGPVTEKIQTRFFDVVNGRAPEYAHWLAHV"
     gene            2214..2666
                     /locus_tag="PKV97_05685"
     CDS             2214..2666
                     /locus_tag="PKV97_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNB05405.1"
                     /translation="MKDRPWKPNVTVAAIIERDGRFLLVEEETPEGLRFNQPAGHLEE
                     GESLLQATVREVLEETAHHFEPEYLVGIYQWTRPQGDITYLRFAYGGRLCGEESGRRL
                     DEGIVRAVWLTLDEVRACADRHRSPLILQCIEDWIAGQRHPLALVHHR"
     gene            2675..3358
                     /locus_tag="PKV97_05690"
     CDS             2675..3358
                     /locus_tag="PKV97_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05406.1"
                     /translation="MAPALRLLLPALLALPATTWAMAGAQGPAGGARVNICYNYGCAS
                     EGSVRVRESTLRRIGERLAAARSAGEERERLAEAVGGLYRVAATQTAIAADRAGNLLD
                     EGADGRMDCIDHSTSTTRLLQLLEARGALRFHRVVEPARRTRLILQHFSAVIEALSVE
                     ERFERLPPGQALAGCNCTEDGLVIGEMDGDDRPGQRYVVDSWFVDNGEPAVVLPLAEW
                     LNGGGPNVQ"
     gene            3387..4484
                     /gene="mnmA"
                     /locus_tag="PKV97_05695"
     CDS             3387..4484
                     /gene="mnmA"
                     /locus_tag="PKV97_05695"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNB05407.1"
                     /translation="MKVVVGMSGGVDSAVTALLLKRQGYQVTGLFMKNWEDDDDSEYC
                     STRQDLVDVASVCDVIGIDLEVVNFSAEYKDRVFADFLHEYEAGRTPNPDVLCNSEIK
                     FRCFLDHAMQLGAERIATGHYAQVREWANDGRSEYQLLKAEDGTKDQSYFLYRLNQEQ
                     LAKTMFPLGALYKREVRRIAAEAGLHVAEKKDSTGICFIGERPFREFLMRYLPTRPGE
                     IRALDDGRVLGEHQGLMYHTIGQRKGLHIGGLKGHQDEAGEHEAWYVAQKDVKANVLY
                     VVQGHAHPALLRDHLVAGELNWIAGRDPHTNWVYTAKPRYRTPDQPCEIDRIVDGRAE
                     IAFAQPQWAVTPGQSVVVYESKVCLGGGIIL"
     gene            complement(4566..5522)
                     /locus_tag="PKV97_05700"
     CDS             complement(4566..5522)
                     /locus_tag="PKV97_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05408.1"
                     /translation="MEECIPDGTVAIIDGVRRIYYDGYWLKAYEPPADTLDAKKRLIQ
                     ALTRRLFNHVEHGINIPGKRLDDARRAYETEEDPARKRVKGAMYAGALFNRATDIFTK
                     LVELQELGIEIATDNPLMRECGGCLQEALKLGRLVLHRSGDEGIDELWGEPFRAFSIP
                     VEAFYESRYIKIAQALREVDRIAAVMALTFAATPLFRGVEPLVFEFARIAKIKCETLR
                     TDPDIFDVWAEFVTASERLAALTPALGPGADECTRQLAADGLHLILQGRNLVTDIIRA
                     RVTMPKSAREFIERCETFAARAQAASHPPLAAADVGLGETRA"
     gene            complement(5575..6141)
                     /locus_tag="PKV97_05705"
     CDS             complement(5575..6141)
                     /locus_tag="PKV97_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNB05409.1"
                     /translation="MLERFLPTREAITQSRMLRWLGPRLHDPLLWHVNRRSVARGVAM
                     GVFFGLMIPIAQIPAAAIASLLLRGNLLIAAASTLVSNPLTYGPLYYFAYQLGAGVIG
                     RRTPAALTADDVEAPVRAIDSLAQAWAWIGGIGQPLLVGMVIMAVTGALVAYWGTQLV
                     WRLRVAARRRRQRRERSARASAQAIGSA"
     gene            complement(6341..6793)
                     /locus_tag="PKV97_05710"
     CDS             complement(6341..6793)
                     /locus_tag="PKV97_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNB05410.1"
                     /translation="MDLLLWRHAEAVDGSPDHTRELTPRGLKQARRMARWLEDNRPKN
                     LKVLVSPTLRTRQTATAFTDDFEIVGGLGPDGNVADILAATGWPEGNGAALVVGHQPA
                     LGRLCALLLSGSEADWTIKKGALWWFTNRVREDETQTVLRAVIPSELV"
     gene            complement(6889..8412)
                     /locus_tag="PKV97_05715"
     CDS             complement(6889..8412)
                     /locus_tag="PKV97_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNB05411.1"
                     /translation="MSHEIELKLALPRRALPALRRHPLVAAAEKCGNALTLDNTYYDT
                     PKLQLKARKVAVRTRRQGLLQTVKCAAVSSGGLSQRPEWETAWTGGFDFTPVDDPATA
                     RLLERHRAELVPVFTTRFRRETRRLVPQAGVSILLMIDTGAVHVRTLEGVEREAEICE
                     LELELESGRAQDLLDLACTLAQDLPLMPADLSKAERGYRLFLDTPAGALRSEISTLEP
                     GQNVVEAFQGLALSCVRQWQGNAATALAQGDPDAIDPDNIHQLRVAHRRLRALLKIFA
                     PALPETFAGTWNARLRDNANRFGDARDLDVFHAELLEPVTPEGLADAASMAALLDTVR
                     TARASARHHAGVSLDLATQGRLLLEFTAALHRLRADSLAEAADLRSFARLRLDRLRKR
                     ARRGARAAASLEPTRLHALRIDFKMLRYGVEFFAPLFGTRSITRYLDGVVRAQTTLGF
                     LQDVDTAHQRLADWSRTQPALAAAAAFVLGWHAPRYARLRRRVLRECEPLLWGGKPW"
     gene            complement(8624..>10650)
                     /gene="glnE"
                     /locus_tag="PKV97_05720"
     CDS             complement(8624..>10650)
                     /gene="glnE"
                     /locus_tag="PKV97_05720"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HNB05412.1"
                     /translation="YFVTQGREWERYAWIKARVMLGERWPELEQLARPFVFRKYLDFG
                     AINAMRELHAQIRREVARRERANNVKLGPGGIREIEFIAQVFQLIRGGRDTALQVRPT
                     LAVLARLPERGILSEEAVTELSNAYVFLRRLEHRLQYLDDAQTHDLPEKDEDRALIAE
                     AMGFADYAALLAVLDAHREVVSRHFDSVFGDPSEEDHSLDATWANARDTEAATGALGE
                     LGYRHPRMAAERLAAMHASPRYRQLPNNIKSRFDALMPRVLEAAASTPGADETLARML
                     DLIEAIGRRGAYLALLQQYPQALRRVADLMSASRWGAQFLTRHPILLDEMLDARSLDN
                     APDWKAFRASLAEELEALEPDMERQMDVMRERHHAQVFRLLTQDLAGLLTVEKLADHL
                     SELADIVLDLTLPLCWRRIKIRHREAPKFAVISYGKLGGKELGYASDLDIVFLHDDEA
                     GEAPEVYTRLAQRVSTWLSSQTAAGQLFETDLRLRPNGESGLLVSSLESFRSYQLESA
                     WVWEHQALTRARFSAGDRTIGEAFERIRCEVLCMRRDLPSLRAEVLAMRHKMRDAHGS
                     RSEEVFDLKHDEGGLIDVEFLIQYLVLGHSHDHPRLTGNLGNIALLGIAGELGLVPAD
                     LAAACADSYRELRRLQHRQRLNERPSRVDPAEAAAARKPVRALWRAVFGD"
BASE COUNT         1533 a         3494 c         4016 g         1607 t
ORIGIN      
        1 ggcggccgtc gcgacccacc gcgatcgcgc gctggccgcg caccgcggct tcggagccga
       61 tcgcgtggcc gatcgcgcgc accgccgtgg gagtcagcac ggtgtcgacg atgccgcgga
      121 tgtcgtaggc cttgaaaagc tctgctgcgg gcagggggtg gcgcatgggc ggttccaggc
      181 agggcgaagg cggggaagct cgcccggcga tgccggcccg ggcggcggtt ggcgcgattc
      241 taggccgcga agcgcgaccg ggtcacgccg ccgcggacga gtcgccgtgc gcgggcgggc
      301 tcagtgcagc acgcgcggcg tcgaatcctc gggacccgag atctcgggca ccggcgaggc
      361 gtggtgcagc accatgcgcc agccgtcggc gccgcgcacg aagacgttgg tcgcggcgat
      421 ggtcgcggta cgcggggtgg gaccgtcggt ggcgaggcgc tcgagcacgc agtgcatcgc
      481 catcatcgcg gtggagctgg tcaccgcatg gctgatgtcg atgcggatgc gggcgccgtc
      541 ggcgaggatc tgccgccaga tctcgcgcac cgcggccagg ccgacgatgc gcgggccgcc
      601 cggctggacg cagacgacct cctcctcttc cgaccagacg gccatcaagg cctcgaagtc
      661 gccctcggag agtgcgcggt agaaggcggc ttccgcttcg agggcggtgg tgaagacagg
      721 tctgctcaag gttgtgttgt tattgtgggt ggatcgtaaa gaccacgggg gccgcagccc
      781 ccgtggtccg gtcgggtcgc gggccattcc ggcccggcac ccgggttctc agtgatggcc
      841 cttggggcgc tcgccgttga acacgtagtg cgcgctgcag tacgggcaga ccgcctcgcc
      901 ggtcttcagg atgtcgagga acacgcgcgg gtggcgggcc cacagcggcg cgcccggcgg
      961 cgggcagtgc agcggcaggt ccttggcggt ggtgacgacg gcctgggtct tgtcggtgtt
     1021 ctcaaccatg acggaatcct cggagcgtgg aaatcagacg tgggcgagcc agtgcgcgta
     1081 ttcgggcgca cggccgttga cgacgtcgaa gaagcgggtc tggatcttct cggtgaccgg
     1141 gccgcgcctg ccttcgccga tctggcggtc gtcgagctcg cggatcgggg tgacctcggc
     1201 ggcggtgccg gtgaagaagg cctcgtcggc gaggtagatg tcgtcgcggg tcaggcgctt
     1261 ggtgccgacc tcgtagccga gttcgcgcgc gatcacgtgg atggagtcgc gggtgatgcc
     1321 ggtgagcgcg gaggcgatct cgggctcgta gatgcggccg tccttgacga tgaagaggtt
     1381 ctcgcccgcg ccctcggcga cgaagccctg attgtcgagc agcagggcct cgtcgtagcc
     1441 catgcgggtg acctcgaggt tggcgaggat cgagttcggg taggtcgccg acagcttggc
     1501 gcgcggcatc gtcgagttga cgtggtggcg ggtgtaggac gaggtcttca cgcggatgcc
     1561 cttctgcagg ccttcctcgc cgaggtaggc gccccagggc caggccgcga tcgcgacatg
     1621 gacgcgcgcg ccgagcgtgg agatgcccat cttttccgag ccgtagaagg cgatcgggcg
     1681 caggtagcag gactcgagct tgttcgcgcg cacgacctcc ttctgcgcct ccatgagttc
     1741 atccttggaa taggggatgt ccatcatgta gatcttgccg gaattgatca ggcgctgggt
     1801 gtgctcggcg aggcggaaga tcgcggtgcc gctgacggtg ttgtaggcac ggacgccctc
     1861 gaacacggcg aggccgtagt ggagcgagtg ggtgaggacg tgggtggtcg cctcgcgcca
     1921 cggaacgagc ttgccgtcct gccagatgaa gccgtcgcgg tcagccatgg acatgatgaa
     1981 gatctccgcc ttgaaaaatg ttcgctggat gggttcgtcg cgcaccctcg atcgtccacg
     2041 gggcgcaaag ccttcggatt ctagcgtaaa gccgtgccga ggacgcgggc cgcgcgcaaa
     2101 gccgcgaccg tcgtcgcaga ccgggcgcca gcgcggtcga cgggcaagcg gtgctcctcc
     2161 atccccggtg gtgcgcgcta gaatgcgggc cttcatgcgc ctggagcatc gaagtgaagg
     2221 atcgtccctg gaaacccaat gtcaccgtcg ccgccatcat cgagcgcgac ggccgcttcc
     2281 tgctcgtcga ggaggagacg cccgaaggcc tgcgcttcaa tcagcctgcc ggccacctcg
     2341 aggagggcga gtccctgctg caggccacgg tgcgcgaggt gctggaagag accgcccacc
     2401 acttcgagcc ggaatacctg gtcggcatct atcagtggac ccgcccccag ggcgacatca
     2461 cctacctgcg cttcgcctac ggcggccgtc tgtgcggcga ggagtccggg cgcaggctgg
     2521 acgagggcat cgtgcgcgcg gtgtggctga cgctcgacga ggtgcgcgca tgcgccgatc
     2581 gccaccgcag cccgctgatc ctgcagtgca tcgaggactg gatcgccggg cagcgccatc
     2641 cgctggcgct ggtgcatcac cgctgaacgg gatgatggcg cctgcgctcc gcctgctgct
     2701 gcccgcgctg ctcgccctgc cggcgacgac gtgggcgatg gcgggggcgc agggccccgc
     2761 cggcggtgcg cgcgtcaaca tctgctacaa ctacggctgc gcgagcgagg gcagcgtgcg
     2821 cgtgcgcgag tccaccctgc gccggatcgg cgaacgcctc gctgcggcac gcagcgccgg
     2881 ggaggagcgc gagcgcctcg ccgaggcggt gggcgggctg taccgggtgg ccgcgaccca
     2941 gacggcgatc gcggccgacc gcgccggcaa cctgctcgac gagggcgcgg acgggcgcat
     3001 ggactgcatc gatcactcga cctccacgac ccggctgctg cagctgctcg aggcgcgcgg
     3061 cgcgctgcgc ttccatcgcg tggtcgaacc ggcgcgccgc acccggctga tcctgcagca
     3121 tttcagcgcg gtgatcgaag cgctgtcggt ggaggagcgc ttcgagcgcc tgccgcccgg
     3181 ccaggcgttg gcgggctgca actgcaccga ggacgggctg gtgatcggag agatggacgg
     3241 cgacgaccgt ccgggacaac gttacgtggt cgacagctgg ttcgtcgaca atggcgagcc
     3301 ggcggtcgtg ctgccgctcg cggaatggct caatggaggt ggaccgaatg tccagtagtc
     3361 ggcatggaag gcaggacggg gcggggatga aggtggtggt cggcatgtcc ggcggggtgg
     3421 actcggcggt gaccgcgctg ctgctgaaac gccagggcta ccaggtcacc gggctgttca
     3481 tgaagaactg ggaggacgac gacgattcgg aatattgctc gacgcggcag gacctcgtcg
     3541 acgtggcttc ggtgtgcgac gtgatcggca tcgacctcga ggtggtcaac ttcagcgccg
     3601 aatacaagga ccgcgtgttc gccgacttcc tgcacgagta cgaggccggg cgcacgccca
     3661 acccggacgt gctgtgcaac tccgagatca agttccgctg cttcctcgac cacgccatgc
     3721 agctcggtgc cgaacgcatc gccaccggcc actacgccca ggtgcgcgag tgggccaacg
     3781 acggccgcag cgagtaccag ctgctcaagg ccgaggacgg caccaaggat cagagctact
     3841 tcctctaccg gctcaaccag gagcagctcg ccaagacgat gttcccgctc ggcgcgctgt
     3901 acaagcgcga ggtgcgccgg atcgccgccg aggccgggct gcacgtggcc gagaagaagg
     3961 actccaccgg catctgcttc atcggcgagc gccccttccg cgagttcctg atgcgctacc
     4021 tgccgacccg gccgggcgag atccgcgcgc tcgacgacgg ccgcgtgctc ggcgagcacc
     4081 agggcctgat gtaccacacc atcggccagc gcaagggcct ccacatcggc ggcctcaagg
     4141 gccaccagga cgaggcgggc gaacacgagg cctggtacgt ggcgcagaag gacgtcaagg
     4201 ccaacgtgct ctacgtggtc cagggccacg cccaccccgc gctgctcagg gaccacctgg
     4261 tcgccggcga gctgaactgg atcgccggac gcgacccgca caccaactgg gtgtataccg
     4321 ccaagccgcg ctaccgcacc cccgaccagc cctgcgagat cgaccgcatc gtcgacggcc
     4381 gcgccgagat cgccttcgcc cagccgcagt gggcggtgac ccccgggcag agcgtcgtgg
     4441 tctacgagtc gaaggtgtgc ctgggcggcg ggatcatcct gtgaggcgcg agcggcctct
     4501 cccgtaggag cgacgcaagt cgcgatgctt ctccgcgcac gcccccgctc gcgcgcgctg
     4561 cttgtttacg cgcgcgtctc gcccaggccc acgtcggcgg cagcgagcgg gggatgcgag
     4621 gccgcctgcg cgcgtgccgc gaaggtctcg cagcgctcga tgaactcgcg cgcgctcttg
     4681 ggcatggtca cgcgtgcgcg gatgatgtcg gtgaccaggt tgcgcccctg caggatcagg
     4741 tgcaggccgt cggcggcgag ctggcgggtg cactcgtcgg cgcccgggcc gagcgccggc
     4801 gtgagcgcgg cgaggcgctc cgaggcggtg acgaactccg cccacacgtc gaagatgtcc
     4861 gggtcggtgc gcaaggtctc gcacttgatc ttggcgatgc gcgcgaactc gaacacgagg
     4921 ggctcgacgc cgcgaaagag cggcgtcgcg gcgaaggtga gcgccatcac ggccgcgatg
     4981 cggtccacct cgcgcagcgc ctgggcgatc ttgatgtagc ggctctcgta gaaggcctcg
     5041 accgggatcg agaaggcgcg gaagggctcg ccccacagct cgtcgatgcc ctcgtcgccg
     5101 ctgcggtgca gcaccaggcg gccgagcttg agcgcctcct gcaggcagcc gccgcattcg
     5161 cgcatgagcg ggttgtcggt ggcgatctcg atgcccagct cctgcaactc caccagcttg
     5221 gtgaagatgt cggtggcgcg gttgaacagc gcgcccgcgt acatcgcgcc cttgacgcgc
     5281 ttgcgcgcgg ggtcctcctc ggtctcgtag gcgcggcgcg catcgtcgag gcgtttgccg
     5341 gggatgttga tgccgtgctc gacatggttg aacaggcgcc gggtgagcgc ctggatcagc
     5401 cgcttcttgg cgtcgagcgt gtccgccggc ggctcgtagg ccttgagcca gtagccgtcg
     5461 tagtagatgc ggcggacgcc gtcgatgatc gcgaccgtac cgtcggggat gcactcttcc
     5521 atggccatgg ccgtccgttg ggcggatgag gggatgcgaa ggatacgccc gcgttcaggc
     5581 gcttccaata gcctgcgcgg atgcgcgcgc gctgcgctcg cggcgctggc ggcgacgacg
     5641 ggcggcgacg cggagccgcc acaccagctg cgtgccccag taggcgacga gcgcgcccgt
     5701 caccgccatg atcaccatgc cgaccagcag cggctggccg atgccgccga tccaggccca
     5761 ggcctgggcg agcgagtcga tggcgcgcac cggcgcctcg acgtcgtccg cggtgagcgc
     5821 ggctggcgtg cggcggccga tcacgcccgc gccgagctgg taggcgaagt agtagagcgg
     5881 accgtaggtg agcggattgc tgaccagcgt ggaggctgcc gcgatcagca ggttgccgcg
     5941 cagcagcagc gaggcgatcg ctgccgccgg gatttgcgcg atcgggatca tcagcccgaa
     6001 gaacacgccc atcgccacgc cgcgtgccac cgagcgccgg ttgacgtgcc acagcagcgg
     6061 gtcgtgcagg cgcggcccca gccagcgcag catgcgactt tgcgtgatgg cctcgcgagt
     6121 gggcagaaag cgttcgagca tggtccgttc cggaaggaag acggcatctt acgcattgca
     6181 tcgcaaagca caagcgagcc ccccacgggc ttcgaggacc gtgcgcgagc ctgccgtccg
     6241 ggatatttca cggcaggttg gaccgtgctc gcggcgagga attcaatggg agcgccaggc
     6301 gtgagcagcg gggcgccccg cgctcctcac gcctggcgcc ttacaccagc tcgctgggaa
     6361 tcaccgcgcg cagcaccgtc tgcgtctcgt cctcgcgcac gcggttggta aaccaccaca
     6421 gcgcaccctt cttgatcgtc cagtcggcct cgctgcccga cagcagcagc gcgcacagac
     6481 ggccaagggc gggctggtgg ccgaccacca gggcggcgcc gttgccctcg ggccagccgg
     6541 tggcggcgag gatgtcggcg acgttgccgt ccgggcccag gccgccgacg atctcgaagt
     6601 cgtcggtgaa ggcggtcgcg gtctggcggg tgcgcagcgt ggggctgacc agtaccttca
     6661 ggttcttcgg ccggttgtct tccagccaac gcgccatgcg acgcgcctgc ttgagaccgc
     6721 ggggggtgag ttcgcgggtg tggtcggggc tgccgtccac cgcttccgcg tgacgccaga
     6781 gcagcaggtc catgtcgttc atccggggtt cgagggcgcg catcatcggc ggctgcggtt
     6841 acagcacgat gaccgcggtc tggcgcgccg ccgccgtggg gcgccgtctc accacggctt
     6901 gccgccccac agcagcggct cgcactcgcg cagcacgcgg cggcgcaggc gggcgtagcg
     6961 cggggcgtgc cagccgagca cgaaggccgc ggccgcggcg agcgcgggct gcgttcgcga
     7021 ccagtcggcc aggcgctggt gcgcggtgtc gacgtcctgc aggaagccga gcgtggtctg
     7081 cgcgcgcacc acgccgtcga gatagcgggt gatgctcctg gtgccgaaca gcggcgcgaa
     7141 gaactccacg ccgtagcgca gcatcttgaa gtcgatgcgc agcgcgtgca ggcgggtggg
     7201 ctccaggctg gctgccgccc gggcgccgcg gcgggcgcgc ttgcgcagcc ggtcgaggcg
     7261 cagacgggcg aagctgcgca ggtcggcggc ttcggcgagg ctgtcggcac gcaggcggtg
     7321 gagtgcggcg gtgaactcca gcagcagccg gccctgggtg gcgaggtcga ggctgacgcc
     7381 ggcgtggtgg cgggcgcttg cgcgcgccgt gcgcacggtg tcgagcaggg ccgccatcga
     7441 ggctgcgtcg gcgaggccct cgggggtcac cggctcgagc agctcggcgt ggaacacatc
     7501 caggtcgcgc gcatcgccga agcggttggc gttgtcgcgc aggcgggcgt tccaggtgcc
     7561 ggcgaaggtc tcgggcaggg cgggtgcgaa gatcttgagc agcgcgcgca ggcggcgatg
     7621 ggcgacgcgc agctggtgga tgttgtcggg gtcgatcgcg tcggggtcgc cctgcgcgag
     7681 cgcggtcgcg gcgttgccct gccactggcg cacgcaggac agtgccaggc cctggaaggc
     7741 ctcgaccacg ttctggccgg gctcgagcgt ggagatctcg gagcgcagcg cgcccgcggg
     7801 cgtgtcgagg aaaaggcggt agccgcgttc ggccttggag aggtcggccg gcatcagcgg
     7861 caggtcctgc gcgagcgtgc aggcgaggtc gaggaggtcc tgcgcgcgcc cgctctcgag
     7921 ttccagctcg agttcgcaga tctccgcctc gcgctcgacg ccctccaggg tgcgcacatg
     7981 cactgcgccg gtgtcgatca tcagcaggat ggacacgccc gcctgcggga cgagtcgccg
     8041 cgtctcgcgg cggaagcggg tggtgaacac cggcaccagc tccgcgcggt ggcgttcgag
     8101 cagacgcgcg gtggcggggt cgtcgaccgg cgtgaaatcg aagccgccgg tccacgcggt
     8161 ctcccactcc ggccgctgcg acagcccgcc gctcgatacc gccgcgcatt tcaccgtctg
     8221 cagcaggccc tggcggcggg tgcgcacggc caccttgcgc gccttgagct gcagcttcgg
     8281 ggtgtcgtag taggtgttgt cgagggtgag ggcgttgccg catttctccg ccgcggccac
     8341 cagcgggtgg cggcgcagcg ccgggagcgc gcgcctgggc agcgcgagct tgagttcgat
     8401 ttcgtggctc atggaggtcg cccgcgggcg ggcgctggaa gacaggtgcc ctggattcta
     8461 gacgccggcc cgcggggcgt catgcgcgct tttgcgacgt gaaatatttc actttcccgc
     8521 gctgggcctg cagcggatcg gtgcccgctg gcgtctcgcg ccgctcgacg gtcgatcgcg
     8581 actcgcgtcg ctcccacggg aaagcgctgc gcagccgggc ggcctagtcc ccgaacacgg
     8641 cccgccacag tgcgcgcacc ggcttgcgtg cggccgcggc ctcggcggga tcgacgcgcg
     8701 agggacgctc gttgaggcgc tggcggtgtt gcaggcggcg cagttcgcgg tagctgtcgg
     8761 cgcaggcggc ggcgaggtcg gcggggacca ggccgagctc gccggcgatg cccagcagcg
     8821 cgatgttgcc gaggttgccg gtgaggcgcg ggtggtcgtg gctgtggccg agcaccaggt
     8881 actggatcag gaactcgacg tcgatcagcc cgccttcgtc gtgcttgagg tcgaacacct
     8941 cctcgctcct gctgccgtgc gcgtcgcgca tcttgtgccg catcgccagc acctcggcgc
     9001 gcaggctggg caggtcgcgc cgcatgcaca gcacctcgca gcggatgcgc tcgaaggctt
     9061 ccccgatcgt gcggtcgccg gccgagaaac gcgcgcgcgt caaggcctgg tgctcccaca
     9121 cccaggccga ctcgagctga tagctgcgga aggactcgag cgagctcacc agcaggccgg
     9181 actcgccgtt cgggcgcagg cgcaggtcgg tctcgaagag ctggccggcg gcggtctggc
     9241 tcgacagcca ggtgctgacg cgctgcgcca gccgggtgta gacctcgggc gcctcccccg
     9301 cctcgtcgtc gtggaggaac acgatgtcga ggtcggaggc gtagccgagc tccttgccgc
     9361 cgagcttgcc gtagctgatc accgcgaact tcggcgcctc gcggtggcgg atcttgatgc
     9421 gccgccagca cagcggcagg gtgaggtcga gcacgatgtc ggcgagctcg gagaggtggt
     9481 cggcgagctt ctcgaccgtg agcaggccgg cgaggtcctg ggtgagcagg cggaagacct
     9541 gggcgtgatg gcgctcgcgc atcacgtcca tctggcgctc catgtcgggt tcgagcgcct
     9601 cgagctcctc ggccaggctg gcgcggaagg ccttccagtc gggggcgttg tccaggctgc
     9661 gcgcgtcgag catctcgtcg agcaggatcg gatggcgagt gaggaactgc gcgccccagc
     9721 gcgatgcgct catcaggtcg gcgacccggc gcagcgcctg cgggtattgc tgcagcagcg
     9781 cgagataggc gccgcggcgg ccgatcgcct cgatgaggtc gagcatgcgc gcgagcgtct
     9841 cgtccgcgcc cggcgtgctg gcggcggctt cgagcacgcg cggcatgagc gcgtcgaagc
     9901 ggctcttgat gttgttgggc agctggcgat agcgcgggct ggcgtgcatc gcggcgagac
     9961 gctcggcggc catgcgcggg tggcggtagc cgagctcgcc gagcgcgccg gtggcggcct
    10021 cggtgtcgcg cgcgttggcc caggtggcat cgaggctgtg gtcttcctcg gaggggtcgc
    10081 cgaacacgct gtcgaagtgc cggctcacca cctcgcggtg ggcgtcgagc accgcgagca
    10141 gcgccgcgta gtcggcgaag cccatggcct cggcgatcag cgcgcgatcc tcgtccttct
    10201 cgggcaggtc gtgggtctgg gcgtcgtcga ggtactgcag gcggtgctcg agccggcgca
    10261 ggaagacgta ggcgttcgac agctccgtca ccgcctcctc cgaaaggatg ccgcgctcgg
    10321 gcaagcgggc gagcaccgcg agcgtcggtc gcacctgcag cgcggtgtcg cgcccgccgc
    10381 ggatgagctg gaagacctgg gcgatgaact cgatctcgcg gatgccgccg gggccgagct
    10441 tgacgttgtt ggcgcgctcg cggcgggcga cttcgcggcg gatctgcgcg tgcagctcgc
    10501 gcatcgcgtt gatcgcaccg aagtcgaggt acttgcggaa cacgaagggg cgcgcgagct
    10561 gttcgagctc gggccagcgc tcgcccagca tcacccgcgc cttgatccag gcgtagcgct
    10621 cccactcgcg gccctgggtg acgaagtagt
//
LOCUS       DAOQNN010000066        10641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2915_length_10641_cov_9.772152, whole genome shotgun sequence.
ACCESSION   DAOQNN010000066 DAOQNN010000000
VERSION     DAOQNN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10641
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2915_length_10641_cov_9.772152"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /gene="ftsY"
                     /locus_tag="PKV97_05725"
     CDS             <1..667
                     /gene="ftsY"
                     /locus_tag="PKV97_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNB05413.1"
                     /translation="DLAGHRPFIIMIAGVNGSGKTTSIGKLAKYFQAHGKSVLLAAGD
                     TFRAAAREQLMTWGERNNVTVVAQDSGDAAAVIFDAINAARARKIDVVLADTAGRLPT
                     QLHLMEEIAKVRRVIAKADPSGPHEVLLVLDANIGQNALAQVKAFDKAIGVTGLVVTK
                     LDGTAKGGVLAAIARQCPKPLRFIGVGEGIDDLQPFKAREFVDALFEPGAGAVKAGAE
                     ARR"
     gene            664..1362
                     /locus_tag="PKV97_05730"
     CDS             664..1362
                     /locus_tag="PKV97_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB05414.1"
                     /translation="MIVFEDVAKRYAGGYTALAGVSFEIRHGELVVLSGHSGAGKSTL
                     FKLIPVIERPTAGRVLINGQDVSHLPRTAIPYLRRNLGLVLQESRLLFDRNVYDNVML
                     PLVITGHPAADAAKRVAAALERVGLDGRGKEMPAGLSGGEQQRVAIARAIVNRPSILI
                     ADEPTAHLDPAYAADIAALFRSFNQAGVTVLVSTHDASLFAASRPRRLVLAKGQLVED
                     SRPGGRPAQEEVAA"
     gene            1359..2255
                     /gene="ftsX"
                     /locus_tag="PKV97_05735"
     CDS             1359..2255
                     /gene="ftsX"
                     /locus_tag="PKV97_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNB05415.1"
                     /translation="MINWFYLHLRAIRHALHRLVSQPVGTLLSALVVGIALSLPGGGY
                     LLLDNLGSLARGVSGTPEISLFLDMNAGAAEVAAIEQRLKGESELASYRFVPRDEGLR
                     QLEAAGLGDVLGGLKANPLPDAFVLAPRREDPALFERMAERMRAWPKVAHVQLDSAWV
                     ERLHALLGLGRSAVLMLAGLLGFALVVVTFNTIRLQILTQRQEIAVSRLLGATDPFIR
                     RPFYWFGGLQGALGGLVALGTMALGIEALAAPVARLAETYGAVFALSGPEPEASLAIV
                     AFAAFLGWLGAAISVRRHLAGT"
     gene            2488..3051
                     /gene="ahpC"
                     /locus_tag="PKV97_05740"
     CDS             2488..3051
                     /gene="ahpC"
                     /locus_tag="PKV97_05740"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HNB05416.1"
                     /translation="MSLINTQVQPFKAQAFHNGKFVEVSDASMKGKWSVVIFMPAAFT
                     FNCPTEIEDAAEHYAEFQKAGAEVYIVTTDTHFSHKVWHETSPAVGKAKFPLVGDPTH
                     QLTRAFGVHIEEAGLALRGTFVIDPEGVIKTMEVHDNAIARDVTETVRKLKAAQYVAS
                     HPNEVCPAKWKEGEATLAPSIDLVGKI"
     gene            3183..4742
                     /gene="ahpF"
                     /locus_tag="PKV97_05745"
     CDS             3183..4742
                     /gene="ahpF"
                     /locus_tag="PKV97_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HNB05417.1"
                     /translation="MLDANIKTQLKAYLERVTQPIEIVASLDDGPKSKELLELLHDVT
                     EQSKLITLVERRDDDERKPSFSVGPRGGEARVRFAGLPMGHEFTSLILALLQAGGYPP
                     KVEAEVIEQIRNLDVELNFETYISLSCHNCPDVVQALNLMAVLNPKIRHTMIDGALFQ
                     KEVEERQIMAVPTVYLNGEEFDQGRLELGQILAKVDTGAAARDARKLSEKPAFDVLVV
                     GGGPAGAAAAIYAARKGIRTGVVAERFGGQVMDTLAIENFISVKYTEGPKLVASLEEH
                     VKEYEVDVMNLQRVAGVVPGENLHEVKMENGASLKAKTVIVATGARWREMNVPGEKEF
                     RGKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGVVAHVTLLEFADDLRADAV
                     LQKKLYSLPNVSVIKSAQTTEVTGADKVNGLVYKDRKTNAEHRLELEGIFVQIGLLPN
                     TDFLRGTLELSRFGEVIVDAKGQTSVPGIFAAGDCTTVPYKQIIIAMGEGAKASLSAF
                     DHLIRSSAPTM"
     gene            4880..6760
                     /locus_tag="PKV97_05750"
     CDS             4880..6760
                     /locus_tag="PKV97_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB05418.1"
                     /translation="MPFSWFERRVDPYPEAAPGPAPRGFFAFLWAGTEGMRPFILGMM
                     LLTATIGAFEALLFAMLGRVVDWLAAVEPARLWADEGGTLLLLAAIILGSIVLVAVQT
                     LLKHQSLAGNFPMRLRWRFHRLMLGQSMSFYQDEFAGRVSAKVMQTALAVRDTCLILT
                     DILVFVSIYFFTMTAVVASFDAWLLAPFLGWLALYVLALRWFVPRLSKVSRAQADARA
                     LMTGRITDAYTNIATVKLFSHAGREAGYARGAMQEFLGTVHAQMRLVSGIEIVNHSLS
                     MGLILATGGTTLWLWSQGQVGVGAVAAATAMALRLNGMSHWVMWEMASLFENVGTVQD
                     GINTLSRPHAVVDRSDAKALVVSRGEVRFERLVFAYGATGPQARRVIDDFTLTIRPGE
                     KIGVVGRSGAGKSTLVNLLLRFYDLEQGRILIDGQDIAGVTQDSLRAQIGMVTQDTSL
                     LHRSVRDNILYGRPDATDADMIAAAKRAEAHEFILGLTDPKGRSGYDAHVGERGVKLS
                     GGQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQASLYRLMEGKTVVAIAHRLS
                     TIAAMDRLVVMDKGRIVEEGDHHSLLARGGLYARLWAHQSGGFLGEEAEDGTPLHPAC
                     PA"
     gene            6828..8579
                     /locus_tag="PKV97_05755"
     CDS             6828..8579
                     /locus_tag="PKV97_05755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB05419.1"
                     /translation="MASESASGDAHPLRVVALIYLCGALLFGTLLVLEWRLIDRSVVA
                     IARERGAALFSLIQTTREWNARHGGVYVVVDESTQPNPWLKHPARDLQTTDGVRLTMV
                     NPAYMTRQIADLALHAEGARLHLTSLKPIRPENRPDAWEAMALARFEAGETEVLALVE
                     GGGEGGSGPAHRYMAPLLVREPCLQCHAEQGYQVGDIRGGISVTMPAEVLIARRDQLR
                     MRAVFVYLLAGLLAAGLMHGLLRANRRHVVQVRRINAEQERLIERRTVELAEANRRLA
                     GEVDLHRRSEQRLAASESRYRAIFDSAAEGIMLLDAALRIVQVNPAFCEITGYAAEEV
                     LGRKPSMLGSGRHDRAFFRELLATLAAAGRWQGEVWNRRKDGDLYVQWMSAVRIGEAD
                     APEGFVATMTDITSRKQTEEKLRFRANHDALTGLPNRRLFEDRLQSAIASALRHGERF
                     ALMLVDLDRFKGVNDRFGHLAGDALLVEVGRRLELCVRASDTVARLGGDEFAVILGEV
                     GGREDAAEVAERICAAMALPFELGEGVAHIGASVGIALGPQDDADAGELQRRADAALY
                     AVKHDGRGGFRFHGEPA"
     gene            complement(8685..10424)
                     /gene="ggt"
                     /locus_tag="PKV97_05760"
     CDS             complement(8685..10424)
                     /gene="ggt"
                     /locus_tag="PKV97_05760"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNB05420.1"
                     /translation="MKRQTLLATALMMAAFGALAKQPVDTGHGGAVATISHEASLAAM
                     QILDAGGNAIDAAVAAAAALGVTDPFSCGIGGGGFMLIYSAKDRRVIAIDHREVAPAS
                     FHPGVFLDAEGKEMDWKATVPNGISVGVPGTVRGWHEALTRYGTMGFDKVLAPAIRVA
                     EEGFAVGPNFHRINKINEAKFARFSTASALYLQDGKALPVGTRFRNPELAQTYRDIAR
                     GGVKAFYEGPIAASMVAAVNHPPVTPGTSVIAGGLTMADLKDYEARIRPPIRSTYRGY
                     EIHGMPAPSSGGIAIAQALNLLETFDLAGKPRAEIEHLYLEASRLAFADRNAYVADEE
                     FVDVPKDGLLSKAYARERARQIGAGAAPGKVAAGDPYPFEDDRSVPLRPQPKPLAQES
                     MHTTHLTVSDREGNVVAYTFTIEDWGGSGIVVPGRGFLLNNELTDFDFSGPHPNLPEA
                     FKRPRSSMSPTIVLKDGAPAFTIGSPGGSTIITTALQTIVNHVDLGMSLADALAAPRM
                     SQRNGETTLVETLLGFPGSEQAKALEAKGHKWRETDQIGAANAIRFNPDGTVTAVSEP
                     LRHGGGSAIVQKQ"
BASE COUNT         1594 a         3739 c         3697 g         1611 t
ORIGIN      
        1 cgacctcgcc ggccatcgcc ctttcatcat catgatcgcg ggcgtcaacg gctccggaaa
       61 gacgacctcg atcggcaagc tggcgaaata cttccaggcc cacggcaaga gcgtgctgct
      121 ggccgccggc gacaccttcc gcgccgccgc gcgcgagcag ctcatgacct ggggcgagcg
      181 caacaacgtc accgtggtgg cacaggacag cggcgacgcc gcggcggtga tcttcgacgc
      241 gatcaacgcc gcgcgggcgc gcaagatcga cgtcgtgctc gccgacaccg ccggccgcct
      301 gcccacccag ctccacctca tggaagagat cgccaaggtg cgtcgcgtga tcgccaaggc
      361 cgacccgagc gggccgcacg aggtgctgct ggtgctcgac gccaacatcg gccagaacgc
      421 gctcgcccag gtgaaggcct tcgacaaggc gatcggcgtc accggcctgg tggtcaccaa
      481 gctcgacggc accgccaagg gcggcgtgct ggcggcgatc gcgcgccagt gccccaagcc
      541 gctgcgcttc atcggcgtcg gcgagggcat cgacgacctg cagcccttca aggcgcgcga
      601 gttcgtcgat gcgctgttcg agcccggcgc cggcgcggtc aaggcaggag cggaagcgcg
      661 cagatgatcg tcttcgagga cgtggccaag cgctatgcgg gcggctacac ggcgctggcg
      721 ggcgtcagct tcgagatccg ccatggcgag ctggtcgtgc tctccggcca ttccggcgcg
      781 ggcaagagca cgctgttcaa gctcatcccg gtcatcgagc gcccgaccgc cggtcgtgtg
      841 ctgatcaacg gtcaggacgt gtcgcacctg ccgcgcacgg ccatccccta cctgcgccgc
      901 aacctcggcc tggtgctgca ggagagccgg ctgctgttcg accgcaacgt ctacgacaac
      961 gtgatgctgc cgctggtgat caccggccac cccgcggccg acgccgcgaa gcgggtggcg
     1021 gccgcgctcg agcgcgtggg cctggacggg cgtggcaagg agatgccggc gggtctgtcg
     1081 ggcggcgagc agcagcgcgt ggcgatcgcg cgcgcgatcg tcaaccggcc gtcgatcctg
     1141 atcgccgacg agcccaccgc ccacctcgac cccgcctacg ccgccgacat cgcggcactg
     1201 ttccgctcct tcaaccaggc cggcgtcacc gtgctcgtgt ccacccacga cgccagcctg
     1261 ttcgccgcca gccggccgcg ccggctcgtg ctggccaagg ggcagctggt cgaggactcg
     1321 cgcccgggcg gacggccggc gcaggaggag gttgcggcgt gatcaactgg ttctacctgc
     1381 acctgcgtgc gatccgtcac gcactgcacc ggctggtgtc gcagcccgta ggcaccctgc
     1441 tgtcggcact ggtggtcggc atcgcgctgt cgctgccggg cggcggctac ctgctcctcg
     1501 acaacctcgg ctcgctggcg cgcggcgtct ccggcacgcc cgagatcagc ctgttcctgg
     1561 acatgaacgc cggcgcggcc gaggtcgccg cgatcgagca gcgcctgaaa ggcgagagcg
     1621 aactcgccag ctaccgcttc gtgccgcgcg acgagggctt gcgccagctc gaggctgcag
     1681 gcctgggcga cgtgctcggc gggctcaagg ccaacccgct gcccgacgcc ttcgtgctcg
     1741 cgccgcgccg cgaggatccg gcgctgttcg agcgcatggc cgagcgcatg cgcgcctggc
     1801 ccaaggtggc gcacgtgcag ctcgactcgg cctgggtcga gcgcctgcat gcgctgctcg
     1861 ggcttggacg ttcggcggtg ctgatgctcg ccggcctgct cggcttcgcg ctggtggtgg
     1921 tcaccttcaa caccattcgc ctgcagatcc tcacccagcg ccaggagatc gcggtgagcc
     1981 ggctgctcgg cgccaccgat cccttcatcc gccgcccctt ctactggttc ggcggcctgc
     2041 agggcgcgct cggcggtctc gtcgcgctcg gcaccatggc gctcggcatc gaggcgctcg
     2101 ccgccccggt ggcgcggctc gccgagacct acggcgccgt cttcgcgctg agcgggcccg
     2161 agcccgaggc cagcctggcg atcgtcgcct tcgcggcctt cctcggctgg ctgggcgcgg
     2221 cgatctcggt gcggcggcat ctcgccggca cttgaacttt cgtcatccgt ccccaattac
     2281 actcgatcgt cgtttgcacg ctgcccgcgg acgttgtccc gggcggtggc ggcgcgatcg
     2341 acgatgcact ccggtaatcg cgagtccata gggacctcca atcgactgga tctccataat
     2401 caattagagc aattcaagga accggtctaa actttgctcc atcgagttgc accgaaccgt
     2461 tccaacaacc cttctggagg attacagatg tcgctgatca acacccaggt tcaaccgttc
     2521 aaagcccagg ccttccacaa cggcaagttc gtcgaagtct ccgatgcgag catgaagggc
     2581 aagtggtccg tggtgatctt catgccggcc gccttcacct tcaactgccc gaccgagatc
     2641 gaggacgcgg ccgagcacta cgccgagttc cagaaggccg gcgccgaggt ctacatcgtc
     2701 accaccgaca cccacttctc gcacaaggtc tggcacgaga cttcgccggc cgtcggcaag
     2761 gccaagttcc ccctggtcgg cgatccgacc caccagctga cccgcgcctt cggcgtgcat
     2821 atcgaggaag ccggcctggc cctgcgcggc accttcgtga tcgacccgga aggcgtcatc
     2881 aagacgatgg aagtgcacga caacgccatc gcccgtgacg tgaccgagac cgtgcgcaag
     2941 ctcaaggccg cccagtacgt cgcctcgcac ccgaacgaag tgtgcccggc gaagtggaag
     3001 gaaggcgaag ccacgctggc cccgtcgatc gacctggtcg gcaagatcta aggcactgct
     3061 gcacctttcc ccggaccgcc gtggtccggg gcgcaagctc cgcagccggc cacgcgccgg
     3121 ctgcgtcgta tccggactgc cgtaaacagc ggcggtcgca aaacagaagg ggagtgacca
     3181 tcatgctgga cgccaacatc aagactcaac tgaaagccta cctggagcgg gtgacgcagc
     3241 cgatcgagat cgtggcgtcg ctggatgatg gtccgaagtc gaaggaactg ctcgagctgc
     3301 tgcacgacgt caccgagcag tcgaagctga tcaccctggt cgagcgtcgc gatgacgacg
     3361 aacgcaagcc ctcgttctcg gtcggtccgc gcggcggcga ggcgcgcgtg cgcttcgcag
     3421 gcctgcccat gggccacgag ttcacctcgc tgatcctcgc cctgctgcag gccggcggct
     3481 atccgcccaa ggtcgaggcc gaggtgatcg agcagatcag gaacctcgac gtcgagctga
     3541 acttcgagac ctacatctcg ctgtcctgcc acaactgccc ggacgtggtg caggcgctca
     3601 acctgatggc ggtgctcaac ccgaagatcc gccacaccat gatcgatggc gcgctgttcc
     3661 agaaggaagt cgaggagcgc cagatcatgg cggtgccgac ggtgtacctg aacggcgagg
     3721 agttcgacca gggccgcctc gagctcggcc agatcctcgc caaggtcgac accggcgccg
     3781 cggcgcgcga cgccaggaag ctctccgaga agccggcctt cgacgtgctc gtcgtggggg
     3841 gcggaccggc cggcgcggcg gcggcgatct acgccgcgcg caagggcatc cgcaccggcg
     3901 tggtggccga gcgcttcggc ggccaggtca tggacacgct ggcgatcgag aacttcatct
     3961 cggtgaagta caccgaaggc cccaagctgg tcgccagcct ggaggagcac gtcaaggaat
     4021 acgaagtcga cgtcatgaac ctgcagcgcg ttgccggcgt ggtgccgggc gagaacctgc
     4081 acgaggtgaa gatggagaac ggcgccagcc tcaaggccaa gaccgtgatc gtcgccaccg
     4141 gtgcgcgctg gcgcgagatg aacgtgcccg gcgagaagga gttccgcggc aagggcgtgg
     4201 cctactgccc gcactgcgac ggcccgctgt tcaagggcaa gcgcgtggcg gtgatcggcg
     4261 gcggcaactc cggcgtcgag gccgcgatcg acctcgccgg cgtggtcgcc cacgtgaccc
     4321 tgctggagtt cgccgacgac ctgcgcgccg acgcggtgct gcagaagaag ctctacagcc
     4381 tgcccaacgt cagcgtgatc aagagcgcgc agaccaccga ggtcaccggt gccgacaagg
     4441 tgaatggcct ggtgtacaag gatcgcaaga ccaacgccga gcaccgtctc gagctcgaag
     4501 gcatcttcgt gcagatcggc ctgctgccca acaccgactt cctcaggggc acgctcgaac
     4561 tgtcgcgctt cggcgaggtg atcgtggacg ccaagggcca gacctcggtg ccgggcatct
     4621 tcgccgccgg cgactgcacc acggtgccgt acaagcagat catcatcgcc atgggcgaag
     4681 gggccaaggc ctcgctgtcc gccttcgacc acctgatccg cagcagcgcg ccgaccatgt
     4741 aagcccggcc cgccgccctc ctgcgcgggt ggcgacagcc gcgaaccccg ccccgccacg
     4801 cgcggggctt tttgctttgc cgcgggcacc cgcggcccat ccggccggtc acaggccgga
     4861 cccatcacgg agagccgccg tgcctttcag ctggttcgaa cgccgcgtcg acccctatcc
     4921 cgaagccgcc cccggccccg cgccgcgcgg cttcttcgcc ttcctgtggg cgggcaccga
     4981 gggcatgcgg cccttcatcc tcggcatgat gctgctcacc gcgaccatcg gcgccttcga
     5041 ggccctgctg ttcgccatgc tcggccgcgt ggtcgactgg ctggccgcgg tcgagcccgc
     5101 gcgcctgtgg gcggacgagg gcggcaccct gctgctgctc gcggccatca tcctcggcag
     5161 catcgtgctg gtggcggtgc agaccctgct caagcaccag agcctggcgg gcaacttccc
     5221 gatgcggctg cgctggcgct tccaccgcct gatgctcggc cagagcatga gcttctacca
     5281 ggacgagttc gccggccggg tgtcggccaa ggtcatgcag accgcgctcg cggtgcgcga
     5341 cacctgcctg atccttaccg acatcctggt gttcgtgagc atctacttct tcaccatgac
     5401 cgcggtggtg gcgagcttcg acgcctggct gctcgcaccc ttcctcggct ggctggcgct
     5461 ctatgtgctg gcgctgcgct ggttcgtgcc gcgcctgtcg aaggtgtcgc gcgcccaggc
     5521 cgacgcgcgc gcgctgatga ccgggcgcat caccgacgcc tacaccaaca tcgccaccgt
     5581 caagctgttc tcccacgccg gccgcgaggc cggctacgcg cgcggcgcga tgcaggagtt
     5641 cctcggcacc gtgcatgcgc agatgcgcct ggtgagcggc atcgagatcg tcaatcacag
     5701 cctgtcgatg gggctgatcc tcgccaccgg cggcaccacg ctgtggctgt ggagccaggg
     5761 ccaggtcggc gtcggcgcgg tggcggcggc gaccgcgatg gcgctgcgcc tgaacggcat
     5821 gtcgcactgg gtgatgtggg agatggcctc gctgttcgag aacgtcggca ccgtgcagga
     5881 cggcatcaac accttgtcgc gcccgcatgc ggtggtcgac cgcagcgacg ccaaggcgct
     5941 ggtggtgagc cgcggcgagg tccgcttcga gcggctcgtg ttcgcctacg gcgccaccgg
     6001 gccgcaggcg cggcgggtga tcgacgactt cacgctgacc atccgtccgg gcgagaagat
     6061 cggcgtggtc gggcgctcgg gcgcgggcaa gtcgaccctg gtgaacctgc tgctgcgctt
     6121 ctacgatctc gagcagggcc gcatcctgat cgacggccag gacatcgccg gcgtgacgca
     6181 ggacagcctg cgcgcgcaga tcggcatggt cacccaggac acctcgctgc tgcaccgctc
     6241 ggtgcgcgac aacatcctct acggccgccc cgacgccacc gacgccgaca tgatcgccgc
     6301 cgcgaagcgg gccgaggcgc acgagttcat cctcggcctt accgacccca agggtcgcag
     6361 cggctacgac gcccacgtcg gcgagcgcgg cgtgaagctc tccggcggcc agcgccagcg
     6421 catcgcgatc gcgcgcgtga tgctgaagga cgcgcccatc ctgctgctcg acgaggccac
     6481 cagcgcgctc gactccgagg tcgaggccgc catccaggcc agcctgtatc gcctgatgga
     6541 aggcaagacc gtggtggcga tcgcgcaccg cttgtccacc atcgcggcga tggatcgcct
     6601 ggtggtgatg gacaagggcc gcatcgtgga ggagggcgat caccacagcc tgctcgcccg
     6661 cggcggcctc tatgcgcggc tgtgggcgca ccagagcggc ggcttcctcg gcgaggaggc
     6721 cgaggacggc acgccgcttc atcccgcctg ccccgcgtga ggaaaggtgt cacgcttcca
     6781 tgcgcgcttt ctccatagaa tgaagcatga ccaccggagg cgatgacatg gcgtcggagt
     6841 ccgcatccgg cgatgcgcat cccctgcgcg tcgtcgcgct catctacctc tgcggcgcgc
     6901 tgctgttcgg aacgttgctg gtgctcgaat ggcggctgat cgaccgcagc gtcgtcgcga
     6961 tcgcccgcga acgcggtgcg gcgctgttct cgctgatcca gaccacccgc gagtggaacg
     7021 ccaggcatgg cggggtgtat gtcgtggtgg acgagagcac ccagccgaac ccttggctga
     7081 agcacccggc gcgcgacctg cagaccaccg acggcgtgcg cctgaccatg gtcaacccgg
     7141 cctacatgac gcggcagatc gccgacctcg ccctgcacgc ggagggcgcg cgcctgcacc
     7201 tcaccagcct caagccgatc cgacccgaga accgtcccga cgcctgggag gcgatggcgc
     7261 tcgcgcggtt cgaggccggc gagaccgagg tgctggcgct ggtggagggc ggcggcgagg
     7321 gcggaagcgg cccggcgcat cgctacatgg cacccttgct ggtgcgcgag ccctgcctgc
     7381 agtgtcatgc agagcagggt taccaggtgg gcgacatccg cggcggcatc tcggtgacga
     7441 tgcctgccga ggtgctgatc gcccggcgcg atcagctgcg catgcgcgcg gtcttcgtct
     7501 atctgctcgc cgggctgctc gccgccggcc tgatgcacgg cctgctgcgt gccaaccggc
     7561 gccatgtcgt ccaggtgcgg cggatcaacg ccgagcagga gcgtctgatc gagcggcgca
     7621 ccgtcgaact cgccgaggcc aaccgccgcc tcgccggcga ggtcgacctg caccggcgca
     7681 gcgagcagcg cctggcggcg agcgagtcgc gctaccgcgc gatcttcgac agtgccgccg
     7741 agggcatcat gctgctcgac gccgcgctgc gcatcgtcca ggtgaacccc gccttctgcg
     7801 aaatcaccgg ctacgcggcc gaagaagtcc ttggccgcaa gccgtccatg ctcggctcgg
     7861 ggcgccacga ccgggccttc ttccgcgagc tcctcgccac gctggccgcc gccggacgct
     7921 ggcagggcga ggtgtggaac cggcgcaagg acggcgacct gtacgtgcag tggatgtcgg
     7981 cggtgcggat cggcgaggcc gacgcgcccg agggcttcgt cgccacgatg accgacatca
     8041 cctcgcgcaa gcagaccgag gagaagctgc gcttccgcgc caaccacgac gcgctcaccg
     8101 gcctgcccaa ccgccgcctg ttcgaggatc ggctgcagtc ggcgatcgcc agcgcgctac
     8161 gccatggcga gcgtttcgcg ctgatgctgg tcgacctcga ccgcttcaag ggcgtcaatg
     8221 accgcttcgg ccacctcgcc ggcgacgcgc tgctggtcga ggtcggccgc cgcctggaac
     8281 tgtgcgtgcg cgcctccgac acggtggcgc gcctgggcgg cgacgagttc gcggtgatcc
     8341 tcggcgaggt cggcggccgc gaggatgccg ccgaggtggc cgagcgcatc tgcgccgcca
     8401 tggccttgcc cttcgagctc ggcgagggcg tcgcgcacat cggcgcctcg gtcggcatcg
     8461 ccctcggtcc gcaggacgac gccgacgccg gggaactgca gcgccgcgcg gacgccgcgc
     8521 tctatgccgt caagcacgat ggccgcggcg gcttccgctt ccacggcgag ccggcctgaa
     8581 ggttcggttt cgacaaggac gccggccctg caggcgcccc cggcaggccg cggccgcgat
     8641 tcgcgccgcg cggcctggtc ttcgcgcccg ggccggtgcc cgcctcactg cttctgcacg
     8701 atcgcgctgc cgccgccgtg gcgcagcggc tcgctgaccg cggtcaccgt gccgtcgggg
     8761 ttgaagcgga tggcgttggc ggcgccgatc tggtcggtct cgcgccactt gtggcccttc
     8821 gcctccagcg ccttggcctg ctcgctgccc gggaagccaa gcagggtctc gaccagggtc
     8881 gtctcgccgt tgcgctggct catgcgcggg gcggcgagcg cgtcggccag gctcatgccc
     8941 aggtcgacgt gattgacgat ggtctgcagc gcggtggtga tgatggtcga gccgccgggc
     9001 gagccgatcg taaacgcggg cgcaccgtcc ttcagcacga tggtcgggct catgctcgag
     9061 cgcgggcgct tgaaggcctc gggcaggttg gggtgggggc cgctgaagtc gaagtcggtg
     9121 agctcgttgt tgagcaggaa gccgcgcccc ggcaccacga tgccgctgcc gccccagtcc
     9181 tcgatggtga aggtgtaggc gaccacgttg ccctcgcggt cggacaccgt gaggtgggtg
     9241 gtatgcatcg actcctgcgc gagcggcttc ggctgcgggc gcagcggcac cgagcggtcg
     9301 tcctcgaagg ggtagggatc gcccgccgcg accttgcccg gcgccgcgcc ggcgccgatc
     9361 tgccgcgcgc gctcgcgcgc gtaggccttc gacagcaggc cgtccttggg cacatccacg
     9421 aactcctcgt cggccacgta ggcgttgcgg tcggcgaagg ccaggcgcga ggcctcgagg
     9481 tagaggtgct cgatctcggc gcgcggcttg ccggcgaggt cgaaggtctc gagcaggttg
     9541 agcgcctgcg cgatcgcgat gccgccgctc gagggcgcgg gcatgccgtg gatctcgtag
     9601 ccgcgatagg tcgaacgtat cggcggacgg atgcgcgcct cgtagtcctt caggtcggcc
     9661 atggtgaggc cgccggcgat cacgctcgtg ccgggcgtga ccggcgggtg gttcaccgcc
     9721 gccaccatgc tcgccgcgat cgggccctcg tagaaggcct tcaccccgcc gcgcgcgata
     9781 tcgcggtagg tctgcgcgag ctcggggttg cggaaacgcg tgcccaccgg cagcgccttg
     9841 ccgtcctgca ggtagagcgc cgacgcggtg gagaagcgcg cgaacttggc ctcgttgatc
     9901 ttgttgatgc ggtggaagtt ggggccgacc gcgaagcctt cttcggccac ccggatggcg
     9961 ggcgcgagca ccttgtcgaa gcccatcgtg ccgtagcgcg tcagcgcctc gtgccagccg
    10021 cgcaccgtgc cgggcacccc gaccgagatg ccgttgggca cggtggcctt ccagtccatc
    10081 tccttgccct cggcgtcgag gaagacgccg gggtggaagc tcgccggggc gacctcgcgg
    10141 tggtcgatgg cgatcacgcg ccggtctttc gcggagtaga tcagcatgaa gccgccgccg
    10201 ccgatgccgc agctgaacgg gtcggtgacg ccgagcgcgg ccgccgcggc caccgcggcg
    10261 tcgatggcgt tgccgccggc gtcgaggatc tgcatcgcgg ccaggctggc ctcgtgcgag
    10321 atggtggcga ccgcgccgcc gtggccggtg tcgaccggct gcttcgccag ggcgccgaac
    10381 gccgccatca tgagcgcggt ggcgaggagg gtttgccttt tcatggtgtg ctccgtcgtg
    10441 ctggggagca tgacggtagc accggccgca gggtatgccc aaggcgggaa tacccgggtg
    10501 gattggggcg aaaggatcgc gcggggaggg cggactgcgc gggccgagag gccgcgatcg
    10561 cgacttgcgt cgctcccaca gcgcccccga cccccgtgcc cgcatgcggg gcgtaggagc
    10621 gacgcaagtc gcgatccggc g
//
LOCUS       DAOQNN010000067        10637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2917_length_10637_cov_9.556700, whole genome shotgun sequence.
ACCESSION   DAOQNN010000067 DAOQNN010000000
VERSION     DAOQNN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10637
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2917_length_10637_cov_9.556700"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKV97_05765"
     CDS             complement(<1..392)
                     /locus_tag="PKV97_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNB05421.1"
                     /translation="MVLSGAEIVIRCLQEEKVEYVFGYPGGAVLFLYDALFQQETVRH
                     VLVRHEQAAVHAADGFARSTEKVGVALVTSGPGATNAVTGIATAYCDSIPMVIISGQV
                     PVAAIGQDAFQEVDTVGITRPCVKHNFLV"
     gene            643..1197
                     /locus_tag="PKV97_05770"
     CDS             643..1197
                     /locus_tag="PKV97_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNB05422.1"
                     /translation="MTRSKSRAGKAVPDGARAAQPPARDASSLKDTAGAGPADRPSAE
                     LAGLRRRLASMLYESMLLLGVLALTFLVPYLMLGIAAGFAPPGWLLWLHVFGVLGGYF
                     VWYWRRGGQTLAMQTWRLKIVTGADGRIASLGRCWLRYALAWPSVLLGGIGLAWALVD
                     RDRQFLHDRLAGTCIVLLPAPAKA"
     gene            1344..3131
                     /locus_tag="PKV97_05775"
     CDS             1344..3131
                     /locus_tag="PKV97_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNB05423.1"
                     /translation="MAGMLAHGPYAEFAVLLIVSALAGAFAVRLRQPVLIAYIVVGIL
                     VGPAVFGIVTAHDEVALLAEIGVTVLLFVVGLKLDLHHIRHIGPVALATGLGQLGFTI
                     VFGFLIIVLMGKSLMDALYVAVALTFSSTIIVVKLLSDKRELDSLHGRIAVGFLIVQD
                     VAVVLAMMTMSALRGHGDDAILAVAGHLALRLVGAGVLMFVLMRYVLPWLVHAMARTQ
                     ELLLIFAIAWGTGLAALGELAGFSKEAGAFLAGFSLASTSYREAMNARLTGIRDFMLL
                     FFFVDLGAKLDFSTLGGELWPAVILSLFVLIGNPLIVMAIMGYMGYRKRTGFLAGLTV
                     AQISEFSIVFVAMGITLGHVGVEALGLTTLVGLVTIMVSTYMILYSQPLYERLAPLLG
                     VFERARPFRELAVERQGRAAGQPDIVVFGLGRYGARLMRQLRQAGIAVIGVDFDPEIV
                     RQLRRQRLPVRFGDGEDPGFLESLPLADARWVVTTFPQWDSNRAFLHALGEAGFKGRI
                     AGVVRDDIHGAALGAAGVERVLNPFNDAADYAARAFAVEIAYEDAHRTDAPAAAGTQS
                     ATPAQDPAAAQPAASPRANAPDRPQETER"
     gene            3128..4024
                     /locus_tag="PKV97_05780"
     CDS             3128..4024
                     /locus_tag="PKV97_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB05424.1"
                     /translation="MNPIRTLVAPTDLSALARHAVVRACLLAAELGARVSLQHVVNSG
                     ALDTLRQLLDTDSVGLQEKLLEEVRGEVEALAAEMHKRHGVTPELHLAVGGVLGEIVA
                     HAEAIDADLLVMGARGAGFMRELLIGSTTERVLRKSVRPMLVVKQIAHEPYRRVLVPV
                     DFSARALEALEFARRVAPQAEFVLLHAFEVPFEGKLRYAGVEESALSSLRVNARREAG
                     AQMNELVARAGVDENRVRRIVVHGEATTQILEQEQEQDCDLIVIGKRGHGLLGEMLLG
                     SVTKHVLARSTADVLVCDRKPD"
     gene            4176..6854
                     /gene="pepN"
                     /locus_tag="PKV97_05785"
     CDS             4176..6854
                     /gene="pepN"
                     /locus_tag="PKV97_05785"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HNB05425.1"
                     /translation="MKTEHAPTIQRADYRPLAWTVETVDLHFRLDPEATLVTNRMSCV
                     RKRDAADGPIRLWSEALERVSLSVDGQAPQALREDGGVIEIDAAGDRVVVEVVTRVNP
                     RANTTLSGLYLSNGGFFTQCEAEGFRRITCFPDRPDVMARYTVTLEADREACPVLLSN
                     GNLVDAGLLPGGRHYAKWEDPFRKPSYLFALVAAKLVALERKVETMSGREVLLQVWVE
                     EGNLDRVEHAMDSLVHSMRWDEETFGLELDLDRFMIVAVADFNMGAMENKGLNIFNTK
                     FILAKPDTATDLDYENVESVVAHEYFHNWTGNRVTCRDWFQLTLKEGLTVFRDQQFSA
                     DMLARAAGPEGAASARAVKRIDDVRVLRAAQFPEDGGPMAHPIRPDSYQEINNFYTAT
                     VYEKGAEVIRMLHTLLGQEGFRNGMDLYFSYFDGQAVTCDDFVEVMSEANNGFDLDQF
                     MRWYEQAGTPRVKASGEWNAADGSYTLTLAQSTPATPGQHVKLPLVIPVALGLIGADG
                     RDLPLRLEGEAQAGATTRVLKLTEEAQVFRFVGLAAEPVPSLLRGFSAPVILELDEDD
                     ARLAFRMAHDADPCNRWDAAQRYAERVALALAADPAATVPEAFVRAFRALLNDDAALD
                     PAFRAQALALPGEAYLLERMSPADPAALRGALVRMMRALGATLAADWLEVVERLQVAG
                     EYRYHPGDAGRRALVNLALRYLAAAGVAEGLARAEARFAAATNMTERFGALAALVHST
                     SPARTAALQAFHDRHRDDALVLDKWFALQAGAWRWGEAAEPALARVRVLLSDPAFSLS
                     NPNKVYALLGTFFRANPGEFHAADGSGHAFWAEQVIALDARNPQVAARMARALENWRR
                     FTPALQASIRPQLKRVLATPGLSADVAEIVGKAVGE"
     assembly_gap    6996..7095
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7323..7769)
                     /locus_tag="PKV97_05790"
     CDS             complement(7323..7769)
                     /locus_tag="PKV97_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB05426.1"
                     /translation="MMTTVKQILEAKGSTFHAVRPTDSVFDALSLMAQFDIGCVLVTD
                     SDKLVGIFTERDYARKVVLKGLVSRDVKVGDLMTPNPYTVGLTGTADDVMATMTAKRF
                     RHIPVVEEGKVLGIVTIGDMVKSIVTQHEKTIKQLESYIAGDLAAE"
     gene            complement(7903..8730)
                     /gene="panC"
                     /locus_tag="PKV97_05795"
     CDS             complement(7903..8730)
                     /gene="panC"
                     /locus_tag="PKV97_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNB05427.1"
                     /translation="MQIHENIDSLRAARAGAGRVAFVPTMGNLHEGHIALMRQAREHA
                     DCVIASIFVNRLQFGAGEDFDRYPRTFAADCEKLAAAGVEHLFAPDESVMYPQPQRYH
                     VDPPAEQVSILEGEFRAGHFRGVATVVMKLLNIVRPEVALFGKKDYQQLMVLSNMVRE
                     LAMPIEVVPGETVRAEDGLALSSRNGYLSADERARAPLLHHLLAGIRAAVRTGERDFA
                     RLEADAMARLSAAGWAPDYVAVRRRLDLQPPVQEDDVLVVLAAAKLGRTRLIDNLEI"
     gene            complement(8760..9575)
                     /gene="panB"
                     /locus_tag="PKV97_05800"
     CDS             complement(8760..9575)
                     /gene="panB"
                     /locus_tag="PKV97_05800"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNB05428.1"
                     /translation="MSYLQDQKPVTLFELGRMRAEGRKIAMLTAYDASFAALLGRAGV
                     DSILIGDSLGNVLQGQKSTLPVTLEQMAYHTECVARAGARPFVVTDMPFGSYHESREQ
                     AMRNAARLMAAGAQMVKLEGGEFMAETVRFLVERGVPVCAHIGLTPQAVHQLGGYRVQ
                     GRSEEGAARLKADALVLEQAGAALMVMEMVPATLAGEITASLSSMATIGIGAGAACDG
                     QVLVLHDLLGVFPGKTARFVRNFMEGAASIEEAVARYAAAVKDGSFPAPEHCY"
     gene            complement(9688..10326)
                     /locus_tag="PKV97_05805"
     CDS             complement(9688..10326)
                     /locus_tag="PKV97_05805"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNB05429.1"
                     /translation="MLDKARYIVVEGPIGAGKTSLARRLADRLQAEAVFEQAELNPFL
                     GRFYQQSERWGLATQLSFLFQRIDQLSTLGPIEGERRVVADFLFDKDHLFAEQNLSPD
                     ELALYRRVHDGLQPANAPRPDLVIYLQARPDTLMERIRKRGLDAERRITELYLEQVAE
                     RYTRYFYQYDAAPLFIVDAGKLNPVDRDEDFELLLERLREMRSYREFFGYAD"
     gene            complement(10355..>10637)
                     /locus_tag="PKV97_05810"
     CDS             complement(10355..>10637)
                     /locus_tag="PKV97_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNB05430.1"
                     /translation="AWYGHLKNMQTKAWYVAALVSWGIALFEYLLQVPANRIGATTLS
                     LAQLKIMQEVITLLVFVPFVVLYMKQPLKLDYLWAALCMLGAVYFIFRN"
BASE COUNT         1480 a         3685 c         3650 g         1722 t
ORIGIN      
        1 acgaggaagt tgtgcttgac gcacgggcgg gtgatgccga ccgtgtcgac ttcctggaag
       61 gcgtcctggc cgatcgcggc gaccggcacc tggccggaga tgatcaccat cgggatcgag
      121 tcgcagtagg cggtggcgat gccggtgacg gcgttggtcg cacccgggcc ggaggtcacc
      181 agcgcgacgc cgaccttctc ggtgctgcgc gcgaaaccgt cggcggcgtg cacggcggcc
      241 tgctcgtggc gtaccagcac gtggcggacc gtttcctgct ggaagagcgc gtcgtagagg
      301 aagaggaccg cgccgcccgg gtaaccgaag acatactcga ccttttcttc ctgcaggcac
      361 ctgattacaa tttcggctcc ggacagcacc atcgcgaact cgctctattg gggtttggat
      421 aaacgcagaa cgttaccggc cgcttgcggt ttggtcaagg ctgcaggcgg tggccgggag
      481 cgcatgcggc gacgcgcgcg cgattgacgt tgtcctgcgg ggccgcgatc gcgatcccgc
      541 cgccggcggc cgcagcgcag gtcctcgcgc acgcgctcct ccccggttcc cctaccgttt
      601 tccctttttg cgccgccgcc atgcggcggc ctggatcgac tcatgacgcg cagcaagagc
      661 cgggccggca aggccgtccc cgacggggcg cgcgccgccc agcccccggc acgggatgct
      721 tcctccctca aggacacggc cggtgcaggc ccagccgacc gcccctccgc cgagctcgcc
      781 ggactgcgcc ggcgcctcgc ttcgatgctg tacgaatcga tgctgctgct cggcgtgctc
      841 gcgctcacct tcctcgttcc ctacctgatg ctcggcatcg ccgccgggtt cgccccgccc
      901 ggctggttgc tctggctgca cgtgttcggc gtgctcgggg ggtatttcgt gtggtactgg
      961 cggcgcggcg ggcagaccct cgcgatgcag acctggcgat tgaagatcgt caccggtgcg
     1021 gacgggcgca tcgcctcgct cgggcgctgc tggctgcgct atgcgctcgc ctggccctcg
     1081 gtgctgctgg gcggcatcgg cctggcgtgg gcgttggtcg atcgcgaccg ccagttcctc
     1141 cacgaccgcc tggccggcac ctgcatcgtg ctgctgcccg cacccgcgaa ggcctgatcc
     1201 ggcgctttcc cgagtttccg cgcgcgcctt ttcgtccgcc ggccctggcg gcgcggatcg
     1261 ggtaccattc gtgcgaaggc tttggttccg ctccgcgcgc ccccgtatcc gggtgtctcg
     1321 ccgggtgaca cgaggatcga acgatggccg ggatgctggc ccacgggccc tacgccgaat
     1381 tcgccgtgct gctgatcgtc tccgccctgg ccggagcgtt cgcggtgcgc ctccgccaac
     1441 cggtgctgat cgcctacatc gtggtcggca tcctggtggg cccggcggtg ttcggcatcg
     1501 tcaccgcgca cgacgaggtc gccctgctcg ccgagatcgg tgtcaccgtg ctgcttttcg
     1561 tggtcgggct caagctcgac ctgcatcaca tccgccacat cggcccggtc gcgctcgcca
     1621 ccggcctcgg ccagctcggc ttcaccatcg tgttcggctt cctcatcatc gtgctgatgg
     1681 gcaaaagcct gatggacgcg ctctacgtcg cggtggcgct gaccttctcc agcacgatca
     1741 tcgtggtcaa gctgctctcg gacaagcgcg agctcgactc cctgcacggg cgcatcgcgg
     1801 tgggtttcct catcgtgcag gacgtcgccg tggtgctggc gatgatgacc atgagcgcgc
     1861 tgcgcggcca cggcgacgac gccatcctcg ccgtggccgg gcacctggcg ctgcgcctgg
     1921 tgggcgccgg cgtgctgatg ttcgtgctga tgcgctacgt gctgccctgg ctggtgcacg
     1981 cgatggcgcg cacgcaggag ctgctgctga tcttcgccat cgcctggggc accgggctcg
     2041 ccgcgctcgg cgagctggcc ggtttcagca aggaggcggg cgccttcctg gccggcttct
     2101 cgctcgcctc gacgagctac cgcgaggcga tgaacgcgcg cctgaccggc atccgcgact
     2161 tcatgctgct gttcttcttc gtcgacctcg gtgccaaact cgatttctcc acgctcggcg
     2221 gtgagctctg gccggcggtg atcctgtcgc tcttcgtgtt gatcggaaac ccgttgatcg
     2281 tcatggcgat catgggctac atgggctacc gcaagcgcac cggcttcctt gccggcctca
     2341 ccgtggcgca gatcagcgag ttctccatcg tgttcgtggc gatgggcatc acccttggtc
     2401 acgtcggtgt agaggcgctc ggtctcacca ccctggtcgg gctggtcacg atcatggtgt
     2461 ccacctacat gatcctgtac tcgcagccgc tctacgagcg cctcgcgccg ctgctcggcg
     2521 tgttcgagcg cgcgcgcccc ttccgcgagc tcgcggtcga gcgccagggc agggcggcgg
     2581 ggcagcccga catcgtggtg ttcggcctcg gccgctatgg cgcgcgcctg atgcgccagc
     2641 tccgccaggc cggcatcgcc gtgatcggcg tcgatttcga ccccgagatc gtccgccagc
     2701 tgcgccgtca gcgtctgccg gtgcgcttcg gcgacggtga ggaccccggc ttcctcgaat
     2761 ccctgccgct cgccgatgcg cgctgggtgg tgaccacctt cccgcagtgg gattccaacc
     2821 gcgccttcct gcacgcgctc ggcgaggccg gcttcaaggg ccgcatcgcc ggcgtggtgc
     2881 gtgacgacat ccatggcgcc gcgctcggcg ccgccggggt ggagcgcgtg ctcaacccct
     2941 tcaacgatgc ggccgactac gcggcgcgcg ccttcgcggt ggagatcgcc tacgaggacg
     3001 ctcaccgcac ggacgcaccc gcagctgccg gcacgcagtc cgcaacgccg gcgcaggatc
     3061 cggccgccgc gcagcccgcg gcgagcccgc gggcgaacgc gcccgaccgg ccccaggaga
     3121 ccgaacgatg aacccgatcc gcaccctcgt cgcccccacc gacctctcgg cgctcgcccg
     3181 tcatgccgtg gtgcgtgcct gcctgctcgc ggccgagctc ggtgcgcggg tgtcgctgca
     3241 gcacgtggtc aattccggcg cgctcgacac cttgcgccag ctgctcgaca ccgactccgt
     3301 cgggctgcag gagaagctgc tcgaggaggt gcgcggcgag gtcgaagcgc tcgccgccga
     3361 gatgcacaag cgccacgggg tgacgcccga gctgcacctg gcggtgggtg gcgtgctcgg
     3421 cgagatcgtc gcccacgccg aggcgatcga cgccgacctg ctggtgatgg gggcgcgcgg
     3481 cgcaggcttc atgcgcgagc tgctgatcgg ctccaccacc gagcgcgtgc tgcgcaagtc
     3541 cgtgcgcccg atgctggtgg tcaagcagat cgcccacgag ccctatcgcc gcgtgctcgt
     3601 gccggtggac ttctcggcac gcgcgcttga ggcgctggag ttcgcgcggc gcgtggcccc
     3661 gcaggccgag ttcgtgctgc tgcacgcctt cgaggtgccc ttcgagggca agctgcgcta
     3721 tgccggcgtg gaggagagcg cgctgtcctc gctgcgcgtc aacgcgcgcc gcgaggcggg
     3781 tgcgcagatg aacgagctgg tcgcgcgcgc gggggtggac gagaaccgcg tgcgccgcat
     3841 cgtggtccac ggcgaggcca cgacgcagat cctcgagcag gagcaggaac aggattgcga
     3901 cctgatcgtg atcggcaagc gcggccacgg cctgctcggc gaaatgctgc tcggcagcgt
     3961 caccaagcac gttctcgcgc gttcgacggc cgatgtgctg gtctgcgacc gcaagcccga
     4021 ctgagccgcg ccgcgggcag cctggcgccg ctcgcgccgt ggtcccgtcg tcctgaccgc
     4081 agctttgcgc caggcatgct cgccctgtgc ggctcgcccg ccagggcgta cactcgcgcc
     4141 cttgccgcgc ccgcgaggcg ccgatggaga acataatgaa aaccgaacat gccccgacga
     4201 tccagcgcgc cgactaccgg ccgctggcgt ggacggtcga gaccgtcgac ctgcacttcc
     4261 gcctcgaccc cgaggccacc ctggtcacca accgaatgtc ctgcgtgcgc aagcgcgacg
     4321 ccgccgacgg cccgatccgg ctatggagcg aggcgctcga gcgcgtgtcg ctgagcgtag
     4381 atggccaggc cccgcaagcg ctgcgcgagg acggcggcgt catcgagatc gacgccgcgg
     4441 gcgatcgcgt cgtggtcgag gtcgtcaccc gggtgaatcc gcgcgccaac accacgctct
     4501 cgggcctcta cctgtcgaac ggaggcttct tcacccagtg cgaggccgag ggcttccgcc
     4561 gcatcacctg tttccccgac cgtcccgacg tgatggcgcg ctacaccgtc acgctcgaag
     4621 ccgaccgcga ggcctgcccg gtgctgctct ccaacggcaa cctggtcgac gcgggcctgc
     4681 tgccgggtgg gcgccactac gccaagtggg aggatccctt ccgcaagccc tcgtatctgt
     4741 tcgcgctggt cgcggcgaag ctggtcgcgc tcgaacgcaa ggtcgagacg atgtcgggcc
     4801 gcgaggtgct gctgcaggtg tgggtggagg agggcaacct cgaccgcgtc gaacacgcga
     4861 tggattcgct ggtgcactcg atgcgctggg acgaggagac cttcggcctg gagctcgacc
     4921 tcgaccgctt catgatcgtc gccgtggccg acttcaacat gggtgcgatg gagaacaagg
     4981 ggctgaacat cttcaacacc aagttcatcc tcgccaagcc cgacaccgcc accgacctcg
     5041 actacgagaa cgtcgagagc gtggtcgcgc acgagtactt ccacaactgg acgggcaacc
     5101 gcgtcacctg ccgcgactgg ttccagctca ccctgaagga aggcctcacc gtcttccgcg
     5161 accagcagtt ctccgccgac atgctggcgc gcgcggccgg ccccgagggc gcggcctcgg
     5221 cgcgcgcggt caagcgcatc gacgacgtgc gcgtgctgcg cgccgcgcag ttccccgagg
     5281 acggtgggcc gatggcgcac ccgatccgcc ccgacagcta ccaggagatc aacaatttct
     5341 acaccgccac ggtctacgag aagggcgccg aggtcatccg catgctgcac accctgctcg
     5401 ggcaggaggg cttccgcaac ggcatggacc tctacttcag ctatttcgac ggtcaggcgg
     5461 tgacctgcga cgacttcgtc gaggtcatgt ccgaggccaa caacggcttc gacctcgacc
     5521 agttcatgcg ctggtacgag caggccggca cgccgcgggt gaaggcgagc ggggagtgga
     5581 acgcggccga cggcagctac acgctgaccc tggcgcaatc gaccccggcc acgcccggcc
     5641 agcacgtcaa gttgccgctg gtaattccgg tcgcgctggg cctcatcggt gcggacgggc
     5701 gcgacctgcc gctgcggctc gagggcgagg cccaggccgg ggcgaccacg cgcgtactga
     5761 agctgaccga ggaggcgcag gtgttccgct tcgtcggcct cgccgccgag ccggtgccct
     5821 cactgctgcg cggcttctcc gcgccggtga tcctcgaact cgacgaggat gatgcgcgcc
     5881 tggccttccg catggcgcac gacgcagacc cctgcaaccg ctgggacgcc gcgcagcgct
     5941 acgccgagcg cgtcgcactg gcgctcgcgg ccgaccccgc ggcgacggtg ccggaggcct
     6001 tcgtccgcgc cttccgcgcc ctgttgaacg acgacgccgc gctcgacccc gccttccgcg
     6061 cccaggcgct cgcgctgccg ggcgaggcct atctgctcga gcgcatgtcg cccgccgatc
     6121 ccgccgcgct gcgcggcgcg ctggtgcgca tgatgcgcgc gctcggcgcc accctcgccg
     6181 ccgactggct ggaggtggtc gagcgcctgc aggtagcggg tgaatatcgc taccacccgg
     6241 gcgacgccgg ccgtcgcgcg ctggtgaatc tcgccctgcg ctacctcgcc gccgccggcg
     6301 tggccgaggg cctggcgcgg gccgaggcgc gtttcgccgc cgccaccaac atgaccgagc
     6361 gcttcggcgc gctcgccgcg ctggtgcaca gcacgagccc ggcgcgcacc gccgcgctgc
     6421 aggccttcca cgaccgccat cgcgacgacg ctctggtact cgacaagtgg ttcgcgctgc
     6481 aggcgggcgc ctggcgctgg ggcgaggccg ccgagccggc gctcgcgcgc gtgcgcgtgc
     6541 tcctcagcga ccccgccttc agcctgtcga acccgaacaa ggtctacgcc ctgctgggca
     6601 ccttcttccg cgccaacccg ggcgagttcc acgccgccga cggcagcggc cacgccttct
     6661 gggccgagca ggtgatcgcg ctcgacgccc gcaacccgca ggtcgccgcc cgcatggccc
     6721 gcgcgctgga gaactggcgt cgtttcacgc ctgcgctgca ggccagcatc cgcccgcagc
     6781 tcaaacgcgt gctcgccacg ccggggctgt cggcggacgt ggccgagatc gtcggcaagg
     6841 cggtggggga gtgagccacg aggcgtccgc tcggcccgac aaaccgaggg ggttcgtagg
     6901 agcgacgcca gtcgcgatcc gtgcgccggg gggccgcgcc ggcgcaactc gaccacagca
     6961 atcgcgactt gcgtcgctcc cacagcgcgg ctcgannnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnggcgc aactcgacca cagcaatcgc gacttgcgtc gctcccacag
     7141 cgcggctcga cgagccgagg cggtttctgt gggagcgacg caagtcgcga tgagcgcgct
     7201 tccgggccgc gctggcgcag ccttcccgcg cgccccgcga tgcatcccgc agacgacgac
     7261 gccggacccg actcccgtcg gctccggcgt ctgcttgttc ggcggcgatg tcgccgcgcg
     7321 gctcactccg cggcgaggtc gccggcgatg tagctctcga gctgcttgat ggtcttctcg
     7381 tgctgggtga cgatggactt caccatgtcg ccgatcgtca cgatgccgag caccttgcct
     7441 tcctcgacca ccgggatgtg gcggaagcgc ttggcggtca tcgtcgccat cacgtcgtcg
     7501 gccgtgcccg tcaggccgac cgtgtatggg ttgggcgtca tcaggtcgcc gaccttcaca
     7561 tcgcgcgaca ccagcccctt gagcacgacc ttgcgggcat agtcgcgttc ggtgaagatc
     7621 ccgaccagct tgtcgctgtc ggtgaccagc acgcagccga tgtcgaactg cgccatcagc
     7681 gacagcgcgt cgaagaccga gtcggtcggg cgcaccgcat ggaaggtgct gcctttcgcc
     7741 tcgaggatct gttttaccgt cgtcatcatc tttccactcc cccttcttcg ttgaaatgcg
     7801 cgccgcaggt cgcggccggg cctttatttg cgggttttac ggtttttaac cagatccgtc
     7861 cgccgatgca agcgcgggcg aacccggatc cagcctcccg cttcagattt ccaggttgtc
     7921 gatcagccgg gtgcggccaa gtttcgccgc ggcgagcaca acgagcacgt cgtcttcctg
     7981 caccggcggc tgcaggtcga ggcggcggcg cacggcgacg tagtcgggcg cccatcctgc
     8041 tgcgctcaag cgggccatcg cgtctgcctc cagccgcgcg aagtcgcgct cgccggtgcg
     8101 caccgcggcg cggatgcctg cgagcaggtg atgcagcagg ggcgcgcgcg cgcgctcgtc
     8161 cgcgctcagg tagccgttgc gcgaggacag cgcgaggccg tcctcggcac gcaccgtctc
     8221 acccggtacg acctcgatcg gcatcgccag ttcgcgcacc atgttcgaca gcaccatgag
     8281 ttgctggtag tccttcttgc cgaacagcgc gacctcgggg cgcacgatgt tgaggagctt
     8341 catgaccacg gtggcgacac cgcggaaatg gccggcgcgg aactcgccct ccaggatcga
     8401 cacctgctct gcgggaggat cgacgtggta gcgctgcggc tgcgggtaca tgaccgactc
     8461 gtcgggcgcg aacaggtgct cgactcccgc ggcggcgagt ttctcgcagt cggcggcgaa
     8521 ggtgcgcgga tagcggtcga agtcctcgcc ggcgccgaac tgcaggcggt tgacgaagat
     8581 gctcgcgatc acgcagtcgg cgtgttcgcg cgcctggcgc atcagggcga tatggccctc
     8641 gtggaggttg cccatggtgg gcacgaaggc gacgcgtccg gcgcccgcgc gtgcggcgcg
     8701 caggctgtcg atgttttcgt ggatctgcat ttgcggttcc tcgcgtgcgc gacgcgggct
     8761 cagtaacagt gctcgggagc ggggaagctg ccgtccttga ccgcggccgc gtagcgcgcg
     8821 accgcttcct cgatgctcgc cgcgccttcc atgaagttgc gcacgaagcg cgccgtcttg
     8881 ccggggaaca cgccgagcag gtcgtgcagc accagcacct ggccgtcgca ggcggcgccc
     8941 gcgccgatgc cgatcgtggc catgctggag aggctggcgg tgatctcgcc ggcgagcgtg
     9001 gcgggcacca tttccatgac catcagtgcg gcgccggcct gctcgagcac gagcgcgtcg
     9061 gccttgaggc gcgccgcgcc ttcctcgctg cggccctgca cgcgatagcc gccgagctga
     9121 tgcaccgcct gcggggtgag gccgatgtgc gcgcacaccg gcacgccgcg ctcgacgagg
     9181 aagcgcacgg tctcggccat gaattcgccg ccttcgagct tgaccatctg cgccccggcg
     9241 gccatcaggc gggcggcgtt gcgcatggcc tgctcgcggc tctcgtggta gctgccgaag
     9301 ggcatgtcgg tgacgacgaa ggggcgcgcc ccggcacggg ccacgcattc ggtgtggtag
     9361 gccatctgct cgagggtgac cggcagggtg gatttctgtc cctgcaggac gttgccgagc
     9421 gagtcgccga tcaggatgga atcgacgccg gcgcggccga ggagcgcggc gaagctcgcg
     9481 tcataggcgg tgagcatcgc gatcttgcgc ccttcggcgc gcatcctgcc gagttcgaag
     9541 agggtgacgg gcttctggtc ctggagatag ctcattcatc gctcctgttg ggaggcggca
     9601 gtgtcggcca aactggtgca ttgcacaagc ctcgcggccc gtgccgggca cgggcagcca
     9661 tcgtcggcgc gcgcccgccg actgcgctca atccgcgtag ccgaagaact cgcggtagct
     9721 gcgcatctcg cgcaggcgtt cgagcagcag ttcgaagtcc tcgtcgcggt cgaccgggtt
     9781 gagctttccg gcatccacga tgaagagcgg tgcggcgtcg tactggtaga aatagcgcgt
     9841 gtagcgctcg gccacctgct cgaggtagag ctcggtgatg cggcgctcgg catccaggcc
     9901 gcgcttgcgg atgcgctcca tcagggtgtc ggggcgcgcc tgcaggtaga tcaccaggtc
     9961 gggacggggc gcgttggcgg gctgcaggcc gtcatgcacg cggcgataga gcgcgagttc
    10021 gtcgggcgac aggttctgct cggcgaagag gtggtccttg tcgaacagga agtcggccac
    10081 cacacgacgc tcgccctcga tggggccgag cgtggagagc tggtcgatgc gctggaacag
    10141 gaaggagagc tgggtcgcca ggccccagcg ctcggactgc tggtagaagc ggccgaggaa
    10201 ggggttgagc tcggcctgct cgaacaccgc ctcggcctgg aggcggtcgg ccagacggcg
    10261 ggcgagggag gtcttgccgg cgccgatcgg gccttcgacg acgatgtagc gggccttgtc
    10321 gagcatgctg ggctgccttt ccggtgagcg tgcgtcagtt gcggaagatg aaatacaccg
    10381 cgccgagcat acacaaggcg gcccacaggt agtcaagctt gagcggctgc ttcatgtaga
    10441 gcacgacgaa gggcacgaag accagcaggg tgatcacctc ctgcatgatc ttgagctggg
    10501 cgaggctgag cgtggtggcg ccgatgcggt tggcgggcac ctgcagcagg tattcgaaga
    10561 gcgcgatccc ccagctcacc agtgcggcca cataccaggc cttcgtctgc atgttcttca
    10621 ggtggccgta ccacgcg
//
LOCUS       DAOQNN010000068        10602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_2930_length_10602_cov_10.436048, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000068 DAOQNN010000000
VERSION     DAOQNN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10602
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2930_length_10602_cov_10.436048"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /gene="cbiB"
                     /locus_tag="PKV97_05815"
     CDS             complement(<1..847)
                     /gene="cbiB"
                     /locus_tag="PKV97_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HNB05431.1"
                     /translation="MPSSLLLVLTLKMAAGLLADRVFGEARRFHPLVGFGRWAGWVER
                     ACRRLFFGTNETGMRLAGLLAWALAVLPWVALALWLRTLHPHAHWGVDSALLYFALGG
                     RSLAEHALAVATPLAAGDLDAARARVGWIVSRDTRALDAEGVAKAATESVLENGNDAV
                     FGALLWFVLGGGAGVVLFRLANTLDAMWGYRTPRHLHFGWAAARIDDLLGWLPARLTA
                     LTYAWLGDTRRALACWRAQAPAWDSPNAGPVMAAGAGALGVRLGGAATYHGRAEHRPA
                     LGEGRG"
     gene            1024..2559
                     /locus_tag="PKV97_05820"
     CDS             1024..2559
                     /locus_tag="PKV97_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNB05432.1"
                     /translation="MSPPITLMVQGTTSDAGKTTLVAGLARVLRRRGIKVAPFKPQNM
                     ALNSAVTIDGGEIGRAQALQALAAGVAPHTDFNPVLLKPSTDIGAQVVIHGRAVASLS
                     ARDYHAYKPTAMAAVMQSWDRLCSAYAAVLVEGAGSPAEINLRDRDIANMGFAEAADV
                     PVVLVADIDRGGVFAHLVGTLELLSPSEQARVKGFVINRFRGDLGLLQSGLDWLEART
                     GRPVFGVLPYLHGLFLDAEDALADSAADVRAGEAALRVIAPVYSRISNHTDLDPLRLH
                     PQVDFRWIGPGQAIPPADLIVLPGSKSVQADLAWLRAQGWETAIRRHLRYGGKLIGIC
                     GGFQMLGRELSDPLGLEGAPGTMAGLGLLEVDTVLEQHKQLRNLRGRLANLSREDGSE
                     CAGEPGAAVTGYEIHMGVSSGPALARPALWLAHGDEAMPTRPDGARSADGQILGTYVH
                     GLFDAPAALAALLAWAGLDAEAQEGSGPVDLAARREADLDRLADAIEAHVDLASLFAP
                     NRL"
     gene            2581..3426
                     /gene="queF"
                     /locus_tag="PKV97_05825"
     CDS             2581..3426
                     /gene="queF"
                     /locus_tag="PKV97_05825"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321992.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HNB05433.1"
                     /translation="MNTDTPRRHGAESSPLGQAVAYRDTYAPELLFPISRQLKRDELG
                     LRADMLPFCGEDLWNAYELSWLDARGKPVVALGEFRVPAASPRLIESKSLKLYLNSFN
                     QQRCASVEEVRATIARDLSAAAGAAVAVVLRPLQERPGRSFGYPEGVCIDALEIDIGR
                     YQPCPETLRAAGPEVEETLYSHLLKSNCLVTGQPDWGTLVVRYRGAAIDREGLLRYVV
                     SFRGHNEFHEQCVERVFCDLMARCKPQELAVWARYTRRGGLDINPFRASRAGLRADEA
                     MEVRQ"
     gene            3533..3841
                     /locus_tag="PKV97_05830"
     CDS             3533..3841
                     /locus_tag="PKV97_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB05434.1"
                     /translation="MKALVAAAVAAGLLSASPAFASEALAKSKNCLACHAVDKKLVGP
                     SYKEVAAKYAGQADAAAMLADKVQKGGVGAWGKVPMPPNPQVNAADAKALVDWILTLK
                     "
     gene            complement(3905..6109)
                     /locus_tag="PKV97_05835"
     CDS             complement(3905..6109)
                     /locus_tag="PKV97_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB05435.1"
                     /translation="MVTPADNGLRPRIDATAGDDADSVRILIVDDDPALRRTFPHALA
                     RPGRLFDECGSIGEAIGRLEKQRYALILLDYRLPDANGLALLDWLSDHQRDEAVIIIS
                     GEDAIDAAIGALRGGADDYVRKPYHVAQLQRAVDGALHKGTLERANKLMSQRLKDSER
                     LHRYLVESSPDMIFTLDGSGRFTYINPRIKALLGYDRSALVKRPFTTLVMPEDLDRID
                     TLLSQPSSLPGESFHVELRLRSRRDGGGEGGSVTVSLTGIPMVTQENDRRVIGLYGVA
                     RDISERKRAEEIISFQAYHDQLTHLPNRVLFKDRLELAIAQAQRRTGALAVMFIDIDR
                     FKLVNDTFGHAEGDLLLRAIASRLSATLRRGDTLARLGGDEFTVLLPDINQPEDAEVI
                     ARKVLDALATPVRLSQGEFRATVSVGIALFPRDGTTAEDLTRHADAAMYQVKRSGKNA
                     FRFFDPELNTRHRDRIALENDLRGAVARGELDLHYQPQVSLATRGIVGLEALLRWNHP
                     GLGAIPPATFIQVAEEVGLISEISNWVLETACAQLARWQAEGVGAPRVSINLSACDFQ
                     RGDVVGRIRACLDRHGVAPSCLEVEITEGLMMDDTADIAAKVRALREAGISVAIDDFG
                     TGYSALAYLQRFPVSTLKIDRSFVRDLEGPMTNPIISAITGIARGFDLDLVAEGVENL
                     EQADALRALGCEVMQGYLFARPAPAGEVPGWLRQPERLFLGAGSADLQAAPA"
     gene            complement(6162..8126)
                     /locus_tag="PKV97_05840"
     CDS             complement(6162..8126)
                     /locus_tag="PKV97_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB05436.1"
                     /translation="MFAANFSAFELQPLPDGASTILRMLAAQTPNWAEIALVAARDPA
                     LSLALLLADPLRAGELQDGLNAALRRRLERIGTDLLRAWLLGLGHVGNQPDETADAAL
                     LRAECALHLAIETEYPRPDEAYLAGLWFGLPRSAGWVSGRRPTLPALVADCGLPASLA
                     DALEFDGMDPACAGAMHPLLGLLAAARRLAGGDWQERITEVAELTGLESASVVALRTD
                     VGYILSGHASYPALPGLPSAGTGAACPTLRLTDDPYRYAGMLGLLTAAFVDLDATTIR
                     DRLAIACPLFGLHTPPVLLGSGEDGRLRPLLGAAAGSPDLLIGELRLRLDDETSCIAL
                     GARSEQPCSHFGDGGPPGRSVADWQVARWLGQPGFHVIPLGGAGDTSVALVATASAQA
                     LDSELRWRYAALLGAAARALRSHARQRDAIAEREAALHQRFRDHVRRIAHEASNPLTV
                     LKTRIDMLAQERSGDSTLQDEMSLLNAELDRIDKLLRSAAELPAETAEMRTCRVPELL
                     LDMRTLYGEPLFGSRQIQLELRAARDVPPAAIPASALKQVLLNLLRNASEALQPGQRL
                     VVSVLPLVNVDGRNCLEIRFVDNGPGLPVERAQDPLSPRPSAKGAEHQGLGLSVVREI
                     LAQWGGTLLCRTQAGAGTSFQIFVPLEQSA"
     gene            complement(8243..9379)
                     /locus_tag="PKV97_05845"
     CDS             complement(8243..9379)
                     /locus_tag="PKV97_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB05437.1"
                     /translation="MSQTTLLTREPVVNKNRAITANRLIAHGPNIASVVEALSAQAAV
                     WPTHHPVFVSLGRLVPTPEIMEWAIPGNVAIEIPVQTLAHPHTQALMARLRDAGIGMC
                     LTWFQPEVALPAGHDWRFVLIDARRHPAPTGSPGLSLAWGLADIGAFRQAVAAGYDGA
                     SGWFFLHGNPPAKSLTPGHAQIVRLLNLVRNNGDIREIEGVLKQDVALSFKLLRYINS
                     AGFGLMVEIQSFRHAVSILGYDALNKWLSLLLVTASRDPSAPALMQTAIARGRFMEEI
                     GGGFFDAVERDNLFITGAFSLLHTLLGTSIQTLLEEMNLPPRVSEALLYGQGDFAPFL
                     HLAQCCESFDPKALQAAVDQLHLSCEQVNRAQLVGLGFADSLHG"
     gene            complement(9393..10406)
                     /locus_tag="PKV97_05850"
     CDS             complement(9393..10406)
                     /locus_tag="PKV97_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HNB05438.1"
                     /translation="MSHRISLQPGNHSFEADDDQTVLEAALAAGLLLPHGCRDGACGA
                     CKGRVLEGEVEHGEHSPGALSEAERAEGLALFCCAKPLGDVMVQARSVTRAGDIQVKK
                     LPCRVQKLERLAEDVMRIELKLPASENFAFRAGQYIDILLADGQRRSFSIANAPHDAR
                     HLELHVRRIDGGRFTGHVFETMMEKEILRFEGPLGSFFLREDSARPIVLVAGGTGFAP
                     IKGIVEHAIRLGLQRPITLYWGARRRDGLYLDALARAWEEVLPGLRYVPVLSDEAWAG
                     RGGLVHQAVLEDFADLSAHEVYVCGAPAMVDAARASFHSERGLPEDAFFADAFTFAQP
                     SAR"
     gene            10428..>10602
                     /locus_tag="PKV97_05855"
     CDS             10428..>10602
                     /locus_tag="PKV97_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB05439.1"
                     /translation="MKRILIVGSGDVARRALPWLVRRFRVFALVRQPEAAAELRALGA
                     VPVAGDLDDRRSLR"
BASE COUNT         1530 a         3634 c         3926 g         1512 t
ORIGIN      
        1 cgccacggcc ctcgcccagt gccgggcggt gctccgcgcg gccgtggtag gtcgccgcgc
       61 cgcccaggcg cacgccgagc gcgcccgcac ccgccgccat caccggcccg gcgttcggac
      121 tgtcccaggc gggcgcctgc gcgcgccagc acgcgagcgc ccgccgggtg tctccgagcc
      181 aggcgtaggt gagcgcggtg aggcgcgccg gcagccagcc gagcagatcg tcgatgcgtg
      241 ccgcagccca gccgaagtgc aggtggcgcg gcgtgcggta gccccacatc gcatcgagcg
      301 tgttcgccag gcggaacagc accacccccg cgccgccacc gagcacgaac cacagcagcg
      361 cgccgaacac cgcgtcgttg ccgttctcca gcaccgactc ggtggccgcc ttggccacgc
      421 cctccgcgtc gagcgcgcgg gtgtcgcggc tcacgatcca gcccacccgc gcgcgcgccg
      481 cgtcaaggtc gccggcggcg agcggcgtcg ccaccgccag cgcgtgctcg gccaggctgc
      541 gtccgccgag ggcgaagtag agcagcgcgc tgtccacccc ccagtgcgcg tgcggatgga
      601 gcgtacgcag ccacagcgcg agcgccaccc aaggcagcac cgcgagcgcc caggcgagca
      661 atcctgccag gcgcatgccg gtctcgttgg tgccgaagaa cagccggcgg caggcccgct
      721 cgacccagcc cgcccagcgc ccgaagccga ccagcggatg gaagcgcctc gcctcgccga
      781 atacgcgatc ggcgagcagg ccggcggcca tcttgagcgt gaggacgagc agcaatgaag
      841 agggcatcgg aacagtcaat aaaggaactt gggcgagtca ccccaagcag cgaacacaac
      901 cgcaagcacc acggccccgc ccgcgccggg ccgggcagcc tgcgcaagcc cgggcgccca
      961 ccgtaccctc ccgaatcggc gataatcccg ccccttcgag caaaacccgc aggattctac
     1021 gccatgtccc ccccgatcac gctgatggtg caaggcacca cctccgacgc cggcaagacc
     1081 acgctggtcg ccggactcgc ccgcgtgctg cgccgacggg gcatcaaggt cgccccgttc
     1141 aagccgcaga acatggcgct caactccgcc gtcaccatcg acggcggcga gatcggccgc
     1201 gcccaggccc tgcaggcgct cgccgccggc gtcgccccgc ataccgactt caatccggtg
     1261 ctgctcaagc cttccaccga catcggcgcc caggtcgtca tccacggccg cgccgtcgcc
     1321 agcctgtcgg cgcgcgacta ccacgcctac aagcccaccg cgatggccgc ggtgatgcag
     1381 agctgggacc gcctgtgcag cgcctacgcg gcggtgctgg tcgagggcgc cggcagcccg
     1441 gccgagatca acctgcgcga ccgcgacatc gccaacatgg gtttcgccga agcagccgac
     1501 gtgccggtgg tgctggttgc ggacatcgac cgcggcggcg tgttcgccca cctcgtcggc
     1561 accctcgagc tgctctcgcc ctccgagcag gcgcgcgtca agggcttcgt catcaaccgc
     1621 ttccgcggcg acctcggcct gctgcagtcc ggcctcgact ggctggaggc gcgcacgggg
     1681 cggccggtgt tcggtgtgct gccttacctg cacgggctct tcctcgacgc cgaggacgcg
     1741 ctcgccgaca gcgccgccga cgtgcgcgcg ggcgaggctg cgctgcgcgt gatcgcgccg
     1801 gtgtactcgc gcatctccaa ccacaccgac ctcgatccgc tgcgattgca cccgcaggtg
     1861 gatttccgct ggatcggccc cgggcaggcg attccgcctg ccgacctgat cgtgctgccc
     1921 ggctccaaga gcgtgcaggc cgacctcgcc tggctgcgcg cgcagggctg ggagacggcg
     1981 atccgccgcc acctgcgcta tggcggcaag ctgatcggca tctgcggcgg cttccagatg
     2041 ctcggccgcg agctgtccga cccgctgggc ctcgaaggcg cgccgggaac gatggccggg
     2101 ctgggcctgc tcgaggtgga cacggtgctc gaacaacaca agcagctgcg caacctgcgc
     2161 ggccgcctcg cgaacctgtc gcgcgaagac gggtccgagt gtgcaggtga acccggtgcg
     2221 gccgtcaccg gctacgagat ccacatgggc gtgagctccg gcccggcgct ggcgaggccg
     2281 gcgctgtggt tggcgcacgg cgacgaggcc atgccgacgc ggcccgacgg cgcgcgctct
     2341 gcggacggtc agatcctcgg cacctacgtc cacggcctgt tcgacgcccc cgcggccttg
     2401 gcggcgctgc tcgcctgggc ggggctggac gcggaggcgc aggaaggatc cgggccggtc
     2461 gacctcgccg ctcgccgcga agccgatctc gaccgcctcg ccgacgcgat cgaggcccat
     2521 gtcgatctgg catccctgtt cgccccgaat cgcctctgaa gtcttgcggg atccgcacga
     2581 atgaacaccg acaccccgcg ccgccacggc gccgaatcct ccccgctcgg tcaggcggtc
     2641 gcctaccgcg acacctacgc gcccgagctg ctgttcccga tttcgcgcca gctcaagcgc
     2701 gacgagctcg gcctgcgcgc cgacatgctg cccttctgcg gcgaggactt gtggaacgcc
     2761 tacgaactct cctggctgga cgcgcgcggc aagccggtgg tggccctcgg cgagttccgc
     2821 gtgccggcgg cttcgccgcg actgatcgag tccaagtcgc tcaagctcta cctgaactcg
     2881 ttcaaccagc agcgctgcgc gagtgtcgag gaagtgcgcg cgaccatcgc gcgcgacctt
     2941 tcggcggcgg ccggcgccgc ggtggcggtg gtgttgcggc cgctgcagga acgccccggg
     3001 cgcagcttcg gctaccccga gggcgtctgc atcgacgcgc tcgagatcga catcgggcgc
     3061 taccagccct gcccggagac gctgcgcgcc gcgggaccgg aggtggagga gacgctgtac
     3121 tcgcacctgc tcaagtcgaa ctgcctggtc accggccagc ccgactgggg taccctggtc
     3181 gtgcgctacc gcggggcggc gatcgaccgc gagggcctgc tgcgctacgt ggtgtccttc
     3241 cgcggccaca acgagttcca cgagcagtgc gtggagcgcg tgttctgcga cctcatggcg
     3301 cgctgcaagc cgcaggagct tgcggtgtgg gcgcgctaca cccgccgcgg cgggctggac
     3361 atcaatccct tccgcgccag ccgggcgggg ctgcgggcgg acgaggccat ggaagtgcgc
     3421 cagtgagcgc aggcgcggca aatgtgcaac atcgtatgcg ggccctgccc aaaccggcgc
     3481 atgcggtggt ttaatacctg ccggtaacaa ccaacaccaa gaggtaagga atatgaaggc
     3541 tctcgttgct gctgcggtcg ctgctggtct cctctccgct tcgcccgctt tcgcgtccga
     3601 ggcgctggcc aagtccaaga actgcctggc ctgtcacgcg gtggacaaga agctggtcgg
     3661 cccttcctac aaggaagtcg ccgccaagta cgccggccag gccgatgccg cggcgatgct
     3721 cgccgacaag gtgcagaagg gcggcgtggg cgcctggggc aaggtcccga tgcctcccaa
     3781 cccgcaggtc aatgccgccg acgccaaggc gctcgtcgac tggatcctga cgctcaagta
     3841 atgccgcatt ccgggccggc gctgccggcc cgcgtgcctc cgaagccggc gcaagccggc
     3901 ttttttacgc cggtgcggcc tgcaggtccg cgctgccggc gccgaggaag agccgttcgg
     3961 gctggcgcag ccagcccggc acctcgccgg ccggggcggg gcgggcgaac aggtagccct
     4021 gcatcacctc gcagccgagc gcgcgcagcg cgtcggcctg ctcgagattc tccacgccct
     4081 cggcgacgag gtcgagatcg aagccgcgtg cgatgccggt gatggccgag atgatcgggt
     4141 tggtcatcgg gccttcgagg tcacgcacga agctgcggtc gatcttcagc gtgctgaccg
     4201 ggaagcgctg caggtaggcg agcgccgagt agccggtgcc gaagtcgtcg atcgccacgc
     4261 tgatgccggc ctcgcgcagc gcgcgcacct tggcggcgat gtcggcggtg tcgtccatca
     4321 tcaggccctc ggtgatctcc acctcgaggc atgacggtgc cacgccgtgg cggtcgaggc
     4381 aggcgcggat cctgccgacc acgtcgccgc gctggaagtc gcaggccgac aggttgatcg
     4441 acacgcgcgg ggcgccgacg ccctccgcct gccagcgtgc gagctgcgcg caggccgtct
     4501 ccagcaccca gttgctgatc tcgctgatga ggccgacctc ttcggccacc tggatgaagg
     4561 tcgcgggcgg aatcgcgccc aggcccggat ggttccagcg caacaaggcc tcgaggccga
     4621 cgatgccgcg cgtcgccagg ctgacctggg gctggtaatg caggtcgagc tcgccgcggg
     4681 cgaccgcgcc gcgcaggtcg ttctccagcg cgatgcggtc gcgatggcgg gtgttgagct
     4741 cggggtcgaa gaagcggaag gcgttcttgc cggagcgctt gacctggtac atcgccgcgt
     4801 cggcgtggcg ggtgaggtcc tcggcagtgg tgccgtcgcg cgggaagagc gcgatgccga
     4861 cgctgaccgt ggcacggaat tcgccctggc tgagccgcac cggggtggcg agtgcgtcga
     4921 gcaccttgcg cgcgatgacc tcggcgtcct cgggctggtt gatgtccggg agcagcacgg
     4981 tgaattcgtc gccgccgagg cgggcgagtg tatcgccgcg gcgcagcgtg gccgacagcc
     5041 ggctcgcgat cgcacgcagc agcaggtcgc cctcggcgtg gccgaaggtg tcattgacca
     5101 gcttgaagcg gtcgatgtcg atgaacatca ccgcgagcgc gccggtgcgg cgctgcgcct
     5161 gcgcgatcgc gagttcgagg cggtccttga acagcacccg gttgggcagg tgggtgagct
     5221 ggtcgtgata ggcctggaag gagatgatct cctcggcgcg cttgcgctcg gagatgtcgc
     5281 gcgccacgcc gtagaggccg atcacgcggc ggtcgttctc ctgcgtgacc atcgggatgc
     5341 cggtcagcga cacggtgacg ctgccgccct cgccaccacc gtcgcgccgc gagcgcaggc
     5401 gcagctcgac gtggaagctc tcgcccggca ggctgctcgg ctggctgagc agggtgtcga
     5461 tgcggtcgag gtcctcgggc atcaccaggg tggtgaaggg gcgcttgacc agcgcactgc
     5521 ggtcgtagcc cagcagggcc ttgatgcgcg ggttgatgta ggtgaagcgg ccgctgccgt
     5581 cgagggtgaa gatcatgtcg ggcgagctct ccaccaggta gcggtgcagg cgctccgagt
     5641 ccttcaggcg ctggctcatc agcttgttgg cgcgttcgag tgtgcccttg tgcagggcgc
     5701 cgtcgaccgc gcgctgcagc tgtgccacgt gatagggctt gcgcacgtag tcgtcggcgc
     5761 cgccgcgcag cgcgccgatc gccgcgtcga tcgcgtcctc gccgctgatg atgatcaccg
     5821 cctcgtcgcg ctggtggtcg gacagccagt cgagcagcgc gaggccgttg gcgtcgggga
     5881 gacggtagtc gagcaggatc agcgcgtagc gctgcttctc caggcggccg atcgcctcgc
     5941 cgatgctgcc gcattcgtcg aagaggcgcc cggggcgggc gagtgcatgc ggaaaggtgc
     6001 ggcgcagggc cggatcgtca tcgacgatca ggatgcggac gctgtcggcg tcgtcgccgg
     6061 cggtggcgtc gatccgaggc cttaacccgt tgtcggccgg agtgaccatg gctcccggag
     6121 gctgcgatgt ggagggtttg ttcggccgca tgatgcaaca ctcaggcgct ttgttccagc
     6181 ggaacgaaga tctggaagct cgtgccggcg cccgcctggg tgcggcacag cagggtgccg
     6241 ccccattgtg cgaggatctc gcgcaccacc gagaggccga ggccctggtg ctccgccccc
     6301 tttgcgctcg ggcgtgggct gagcggatcc tgggcgcgtt ccaccggcag gccggggccg
     6361 ttgtcgacga agcggatctc caggcagttg cggccgtcga cattgaccag cggcagcacc
     6421 gagacgacca ggcgctggcc gggctggagc gcttcggagg cgttgcgcag caggttgagc
     6481 agcacctgct tgagcgccga tgcggggatc gcggccggcg gcacgtcgcg cgcggcgcgc
     6541 aactcgagct ggatctggcg gctgccgaag agcggctcgc cgtacagcgt gcgcatgtcc
     6601 agcagcagct cgggcacccg gcaggtgcgc atctcggccg tctcggcagg cagttcggcg
     6661 gcgctgcgca ggagtttgtc gatgcggtcg agttcggcgt tgagcaggct catctcgtct
     6721 tgcagcgtgc tgtcgccgga gcgctcctgg gcgagcatgt cgatgcgggt cttgagcacc
     6781 gtgagcgggt tgctggcctc gtgggcgatg cggcgcacat ggtcgcggaa gcgctggtgc
     6841 agcgcggcct cgcgctcggc gatcgcatcg cgctggcggg cgtggctgcg cagcgcgcgc
     6901 gccgcggcac cgagcagggc ggcgtagcgc cagcgcagct cgctgtcgag cgcctgcgcg
     6961 gaggcggtgg cgacgagggc cacgctggtg tcgcccgcgc cgcccagagg gatgacgtgg
     7021 aagcccggct ggccgagcca gcgtgcgacc tgccagtcgg cgacgctgcg cccgggcggg
     7081 ccgccgtcac cgaaatgcga gcagggttgc tcgctgcgcg caccgagggc gatgcagctg
     7141 gtctcgtcgt ccaggcgcag gcgcagctcg ccgatcagca ggtcggggct gcccgccgcg
     7201 gcgccgagca gcgggcgcag gcgaccgtcc tcgccgctgc cgagcagcac cggcggggtg
     7261 tgcaggccga acagcgggca ggcgatcgcc aggcggtcgc ggatggtcgt cgcgtccagg
     7321 tcgacgaagg cggcggtgag caggccgagc atgccggcgt agcggtaggg gtcgtccgtg
     7381 aggcgcaggg tcgggcaggc cgcgcccgtg cccgcgctgg gcaggccggg cagggcgggg
     7441 taggacgcat gcccggacag gatgtagccg acgtcggtgc gcagcgccac cacgctcgcg
     7501 ctctcgagtc cggtcagctc ggcgacttcc gtgatgcgct cctgccagtc gccaccggcg
     7561 agccggcgtg cggcggcgag caggccgagc agcggatgca tcgcgcccgc gcaggccgga
     7621 tccatgccgt cgaactcgag cgcgtcggcc aggctcgcgg gcaggccgca gtcggcgaca
     7681 agggccggca gcgtcggccg gcgcccggaa acccaccccg ccgagcgcgg caggccgaac
     7741 cacaggccgg cgaggtaggc ctcgtcgggg cgcggatatt cggtctcgat cgcgaggtgg
     7801 agcgcgcatt cggcacgcag cagtgcggcg tcggcggttt cgtccggttg gttgccgacg
     7861 tggcccagac cgagcagcca ggcgcgcagc aggtcggtgc cgatgcgctc gaggcggcgc
     7921 cgcagggcgg cattgaggcc gtcctggagt tcgcccgcgc gcagcggatc ggccagcagc
     7981 agcgccaggc tcagcgcggg gtcgcgtgcg gccacgagcg cgatctccgc ccagttcggc
     8041 gtctgcgcgg cgagcatgcg caggatcgtc gacgcgccgt cgggcagggg ctggagctcg
     8101 aaggcgctga aattggcggc gaacatgcga tggacggttg gaaaaagggc cgacggattc
     8161 tagcccgtcg gcccttcccc ttgccgtgaa cgatgtggcg gtcgagcggg aatgacgaac
     8221 ggggtgcggc gctggacgag gttcagccgt gcaggctgtc ggcgaaaccg agcccgacca
     8281 gctgtgcgcg attgacctgc tcgcagctca ggtggagctg gtccaccgcc gcctgcaagg
     8341 ccttggggtc gaagctctcg cagcactgcg caaggtgcag gaagggggcg aagtcgcctt
     8401 ggccgtagag gagcgcctcg ctcacccgcg gcggcaggtt catctcttcg agcagggtct
     8461 ggatcgaggt gccgagcagg gtgtgcagca gcgagaaggc gccggtgatg aagaggttgt
     8521 cgcgctctac ggcatcgaag aagccgccgc cgatctcttc catgaagcgg ccgcgcgcga
     8581 tcgcggtctg catcagcgcg ggggcgctgg ggtcgcggct ggcggtgacc agcagcagcg
     8641 acagccattt gttgagggcg tcgtagccga ggatgctgac cgcgtggcgg aaggactgga
     8701 tctcgaccat gaggccgaag ccggccgagt tgatgtagcg cagcagcttg aaggagagcg
     8761 ccacgtcctg cttgaggacg ccctcgatct cgcggatgtc gccgttgttg cgcaccaggt
     8821 tgagcagccg cacgatctgc gcatgccccg gcgtgaggct cttggcgggc gggttgccgt
     8881 gcaggaagaa ccagcccgag gcaccgtcgt agcccgccgc gacggcctgg cggaaggcgc
     8941 cgatgtcagc caggccccag gccaggctca ggccgggcga gccggtgggc gcagggtggc
     9001 ggcgcgcgtc gatgagcacg aaacgccagt cgtggccggc gggcagggcg acctcgggct
     9061 ggaaccaggt caggcacatg ccgatgccgg catcgcgcag gcgcgccatc agcgcctggg
     9121 tgtgcggatg ggcgagcgtc tgcaccggga tctcgatcgc cacgttcccg gggatcgccc
     9181 actccatgat ctcgggcgtc ggcaccaggc ggccgaggct gacgaacacc ggatggtggg
     9241 tcggccacac cgcggcctgg gcggagagcg cctcgaccac cgaggcgatg ttggggccgt
     9301 gggcgatcag gcggttggcg gtgatcgcgc ggttcttgtt gacgaccggc tcgcgggtca
     9361 gcagggtggt ctggctcatc gggcgttccg ggtcatcggg cggagggctg ggcgaaggtg
     9421 aaggcgtcgg cgaagaacgc gtcctcgggc aggccgcgtt cgctgtggaa gctggcgcgt
     9481 gcggcatcga ccatcgcggg ggcgccgcag acatagacct cgtgcgcgga aaggtcggcg
     9541 aagtcttcga gtaccgcctg gtgcaccagg ccgccgcgtc ccgcccaggc ctcgtcggag
     9601 agcaccggga cgtagcgcag gccgggcagc acctcctccc aggcgcgtgc gagcgcgtcg
     9661 agatacagcc cgtcgcgccg gcgcgcaccc caatacagcg tgatcgggcg ctgcaggccg
     9721 agccggatcg cgtgctcgac gatgcccttg atcggtgcga agccggtgcc gccggccacc
     9781 agcacgatcg gccgcgcgga gtcctcgcgc aggaagaagc tgcccagcgg cccttcgaag
     9841 cgcaggatct ccttttccat catggtctcg aagacgtggc cggtgaaacg gccgccgtcg
     9901 atgcggcgca catgcagctc gagatggcgg gcgtcgtgcg gggcgttggc gatcgagaag
     9961 ctgcggcgct ggccgtcggc gagcaggatg tcgatgtact ggccggcgcg gaaggcgaag
    10021 ttctcgctcg cgggcagctt gagctcgatc cgcatcacgt cctcggccag gcgctcgagc
    10081 ttctgcacgc ggcagggcag cttcttcacc tggatgtcgc cggcgcgcgt cacgctgcgt
    10141 gcctgcacca tcacgtcgcc gagcggcttc gcgcagcaga acagcgccag cccctcggcg
    10201 cgctcggcct cgctcagtgc gccgggcgag tgctcgccgt gctcgacctc gccctcgagc
    10261 acgcgaccct tgcaggcgcc gcaggcgccg tcgcggcagc cgtgcggcag cagcaggccg
    10321 gcggcgagcg cggcctcgag cacggtctgg tcgtcgtcgg cctcgaagga gtggttgccg
    10381 ggctggaggg aaatccggtg gctcatggtg cgtgagataa tcgaacgatg aaaaggatac
    10441 tgatcgtcgg cagcggtgat gtcgcccgcc gcgcgctgcc ctggctggtg cgtcgctttc
    10501 gtgtcttcgc gctcgtccgc cagcccgagg cggccgccga actgcgtgcg ctcggcgcgg
    10561 tgccggtcgc gggcgacctc gacgaccgcc gcagcctgcg cc
//
LOCUS       DAOQNN010000069        10365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3031_length_10365_cov_11.820951, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000069 DAOQNN010000000
VERSION     DAOQNN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10365
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3031_length_10365_cov_11.820951"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PKV97_05860"
     CDS             complement(<1..1004)
                     /locus_tag="PKV97_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNB05440.1"
                     /translation="MKTSRRTFTMLRLAAALALLGQGAAASAQALDDATLQALATRFD
                     AIQPRMVEWRRDIHQHPELSGQELRTAALVAEHLRKLGMEVRTGVGGHGVVGLLQGGR
                     PGKVVALRADMDALPVAEVTGLPFASQVKQMNMGVESPVMHACGHDGHTAMLMAVAEV
                     LAGMREQIPGTVKFVFQPSEEGLSSAPVAGKSWGAKAMVEEGALDNPRPDVAFALHII
                     PNMPSGMIGYRSGPQFASGDTVRIKIKGKQTHGGFPWNGVDPIVVSAQVVTALQTIVS
                     RQLNISKEPAVLSIGQINGGNRENIVPEAVEMVGTLRAFDEAMRADAKERITRTAESI
                     A"
     gene            complement(1103..2395)
                     /locus_tag="PKV97_05865"
     CDS             complement(1103..2395)
                     /locus_tag="PKV97_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNB05441.1"
                     /translation="MTVGLLSLLAVLVLAFLRVPLAFALLSVSLGGIGVVMGWDVARS
                     LVPMTISEAVFSYELAVVPMFILMGNILARTGISDDLFRAANAFLGPVRGGLALSTMV
                     TCAGFSAVCGSSLATAATMSKVAYPSMKRYGYSDALASATIAAGGTLGILIPPSIILM
                     IYGILTQTNIGHLFVAGVVPGLLGLLMYLLVIYAVARISPQDAPRGERTTMAEKLHAL
                     RGVWPFTLLFVLIIGGLYGKLFTATEAAGMGAGLALILSMVQRRMSWQDFRHIFIETA
                     TTSVMLYSVLFGALLFAKLISFSGLGEGILELFNQAGLGPWGTIVAILVVFLLLGCVM
                     DSLAIILICVPLFVPILLAHGFDLVWFGIIVVVVTEIALITPPIGMNVFVLKATLPHV
                     RLGAIFRGLTPFIAIDLVRLALLVIFPSISLALVAFMK"
     gene            complement(2392..2904)
                     /locus_tag="PKV97_05870"
     CDS             complement(2392..2904)
                     /locus_tag="PKV97_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNB05442.1"
                     /translation="MDAYLSRIRKGVEALLILTLAGMVLLTFIDVIGRRLFGAPLYGA
                     HDATEHLMAIMVFCGLPLLTAARGHLTVDLFDKWLLRPGWVWWHRLVSALVALVLLLI
                     AYEFLNSAIEAGKIQEISQSLSIPRGGMYGFIAFTSLLSALAALLGCFAAPPSHPVTV
                     QSHATQERAK"
     gene            complement(2964..4001)
                     /locus_tag="PKV97_05875"
     CDS             complement(2964..4001)
                     /locus_tag="PKV97_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNB05443.1"
                     /translation="MKLSKLFATLSAGLVLATAAGLPAAQAATTLTMSSWVSPTHFLT
                     PEVFQPWIEDVKRVTEGRVDVRILPKPVGAPAQHWELARKGVADITWGNFTYEPDRFK
                     AVWFSEMPFIGTKAEASSVALWETYEKYLAGKPAFEGVVMLGVGLFGGGQIHHGSKNI
                     VEPDDLKNQKVRMGGPIQKRLLEDLGAVAVAGPGPKAYELLESGVVDASLHTIESVIN
                     FRVEDKLKHHTIVPGGFYDASFFVVINEGKWNKLSEADRKAIMSVSGEKLSRLWGQRF
                     DAQNKAGEAKLRAEGHVFNAPSKALFERIGAVRERMLADWAAEGPSFGVDKPMEMLEF
                     FEQRYKAHAGK"
     gene            complement(4020..5249)
                     /locus_tag="PKV97_05880"
     CDS             complement(4020..5249)
                     /locus_tag="PKV97_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HNB05444.1"
                     /translation="MVSRMTTQEAVAHEIDAEQMKRWRHAIHRNPELGFEEHATSALV
                     AEQLRAWGYEVHTGIAVTGVVGVLRVGDGSGRTIGLRADMDAIPVQEMTGLPWASIVP
                     GKMHGCGHDGHTAMLLGAARALAARVKGGALQGDGTLVLIFQPAEELGGGGGARRMIE
                     DGLFERFPCDAIFGMHNFPGIPVGQVHVRPGAFMASSDKVLIRLIGRGGHGGMPHMAV
                     DPTLPAAATVLGLQSIVGRNVNPNLAAVISVGRLQAGAAYNVIAESAELELSVRTLDP
                     EVRDLVERRIRELAENQARAYGCKTEIRYERGYPVLVNAARPTAFAVEVARQTFGAEH
                     VVADAQPLTGSEDFAFLLEQVPGCYLLVGNGDNGHAEGRWSGECMVHNPHYDFNDACL
                     AVGARLWIGLVEQYFHN"
     gene            5344..6249
                     /locus_tag="PKV97_05885"
     CDS             5344..6249
                     /locus_tag="PKV97_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB05445.1"
                     /translation="MKDHQLKALVQVAESGSIRAAARAMNLSQSALTKALRELEEDVG
                     AELLQRSYKGIGFTPEGSALLVRARLVLATIDKARAEIRQMRGGAGAHVKAAITPLLT
                     ATVLPAVLSAFRKAQPDAELTFHEGLLVQALPGLIEGKIDFAIALASPQDLPYEIEFE
                     PLADIDAVPMVRATHPLAEARDWNAVADAEWVLNLSAGSQSMNLVGWLRARGFRAPTR
                     ILHCSSPYLILDMVRRNDLIGYGPKVLVTDAQVGVGLRPLALQPLPPTMPLGLMTLRG
                     VPLGSAAKRLANLCRRELQSCLPRP"
     gene            complement(6259..7224)
                     /locus_tag="PKV97_05890"
     CDS             complement(6259..7224)
                     /locus_tag="PKV97_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB05446.1"
                     /translation="MDRYTELRSFVLVAEKGSFAAAALVEGVTPVVMGRRLDALEKRL
                     GVKLMHRSTRGLALTELGEQLLDQSRALLREFDELERAVGAGRDLVRGHLVVSAPAAF
                     GRRHVAPHATAFKALHPELRMSFNFTDSVVDLVREGYDMAIRIGEVTDPNYVAVRLFP
                     NRRVVCGTPDYFERHGIPRQPEDLARHNCLAFNLAGGQQRGWSFLRDGRLFAVRVEGD
                     LACNDGELLYGWVKQGLGVGWRSTWEIQAELKRGELVTVLDEFAVPSYDIQAVYPQQR
                     HLPAKVRRFIEHLRASYNRPGYWEERGEERGEERGEERGEERGEE"
     gene            7550..9325
                     /gene="gcl"
                     /locus_tag="PKV97_05895"
     CDS             7550..9325
                     /gene="gcl"
                     /locus_tag="PKV97_05895"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HNB05447.1"
                     /translation="MARMRAVDAAAAVMRKEGVSTVFGVPGAAINPLYSAMKKDGGFR
                     HILGRHVEGASHMAEGYTRANPGNIGVCIGTSGPAGTDMITGLYSASADSVPILCITG
                     QAPRARLHKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLI
                     DLPFDVQMAEIEFDIDTYESLGAYKPAATRAQAEKAMAMLNAAERPLIVAGGGVINAD
                     AADRLQEFAELVGVPVIPTLMGWGAIPDDHPLMAGMAGLQTSHRYGNATMLASDFVFG
                     IGNRWANRHTGSVEVYTRGRKFVHVDIEPTQIGRVFGPDYGIVSDAGAALDRLLEVAH
                     GMKAAGTLPDRGAWVAECQQRKRTLQRKTHFDQVPMKPQRVYEEMNLAFGKDACYVST
                     IGLSQIAAAQFLHVYKPRHWINCGQAGPLGWTIPAALGVCAADPGRKVVAISGDYDFQ
                     FMIEELAVGAQFKLPYLHVLVNNAYLGLIRQSQRGFDMDFCVQLAFENINAPETEGYG
                     VDHVGVVEGLGCKAIRVRTPEEIQPAFAQAKQWMEEFRVPVVVEIILERVTNIAMGTE
                     IDAINEFEELAERGEDAPTAISMLD"
     gene            9403..10182
                     /gene="hyi"
                     /locus_tag="PKV97_05900"
     CDS             9403..10182
                     /gene="hyi"
                     /locus_tag="PKV97_05900"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HNB05448.1"
                     /translation="MPKFNANLTMLFNEVPFLDRFQAAAEAGFKGVEFLFPYAFDKDA
                     LAERLARHGLVQVLHNLPAGDWEGGERGIACHPDRVGEFQDGVGRAIEYARALGCKQV
                     NCLAGIAPAGVEADKVHATFVANLRFAAARLEEAGIRLLVEPINTFDIPGFYLNRTAQ
                     AIALIEEVGSPNLWLQHDLYHMQRMEGELANTIAKHLPKIAHMQIADTPGRHEPGSGE
                     INYAWLFRFIDQLGYEGWIGCEYKPAAGTREGLGWIRALAG"
BASE COUNT         1744 a         3561 c         3503 g         1557 t
ORIGIN      
        1 gcgatcgact cggcggtgcg cgtgatgcgc tccttggcgt cggcgcgcat ggcctcgtcg
       61 aaggcgcgca gcgtgcccac catctccacc gcctcgggga cgatgttctc gcggttgccg
      121 ccgttgatct ggccgatcga cagcaccgcc ggctccttgc tgatgttgag ctggcggctg
      181 acgatggtct gcagcgcggt gacgacctgg gcggagacga cgatcggatc gacaccgttc
      241 cacgggaagc cgccgtgggt ctgcttgccc ttgatcttga tgcgcacggt gtcgccgctg
      301 gcgaactgcg ggccgctgcg gtagccgatc atgcccgagg gcatgttggg gatgatgtgc
      361 agcgcgaagg cgacgtcggg gcgcgggttg tcgagcgcgc cctcctccac catggccttg
      421 gcgccccagc tcttgccggc taccggcgcc gacgagaggc cttcttcaga gggctggaac
      481 acgaacttga ccgtgcccgg gatctgctcg cgcatgccgg ccagcacctc ggccaccgcc
      541 atcagcatcg cggtgtggcc gtcgtggcca caggcgtgca tcaccggcga ctccacgccc
      601 atgttcatct gcttgacctg cgacgcgaag ggcagcccgg tgacctcggc caccggcagc
      661 gcatccatgt cggcgcgcag cgcgacgacc ttgcccggcc gcccgccctg gagcaggccg
      721 accacgccgt ggccgccgac gccggtgcgc acctccatgc cgagcttgcg caggtgctcg
      781 gcgaccagcg ccgcggtgcg cagctcctgg ccggatagtt cggggtgctg gtggatgtcg
      841 cgccgccact cgaccatgcg cggctggatg gcgtcgaagc gggtggcgag cgcctgcagg
      901 gtggcgtcgt cgagcgcctg ggcggaggcg gcagcgccct ggccgagcag ggcaagggcc
      961 gcggcgaggc gcagcatcgt gaatgttcgc ctggatgttt tcattttgga ttctctctga
     1021 gcaaggggtg gggaaggccg gccacgatca cgcgcaacgg gcgcggccga cctggaaagc
     1081 gtcggtcgcg cccgctttca aactatttca tgaacgccac cagcgccagc gagatcgacg
     1141 ggaagatcac cagcagcgcc agccgcacca ggtcgatcgc gatgaagggc gtgaggccgc
     1201 ggaagatcgc gccgagccgc acgtggggca gcgtggcctt gagcacgaac acgttcatgc
     1261 cgatcggcgg cgtgatcagc gcgatctcgg tcacgacgac gacgatgatg ccgaaccaca
     1321 ccaggtcgaa gccgtgggcg agcaggatgg gcacgaacag gggcacgcag atcaggatga
     1381 tggccagcga atccatcacg cagcccagca gcaggaacac gaccaggatc gcgacgatgg
     1441 tgccccaggg gccgaggccc gcctggttga agagctcgag gatgccctcg cccaggcccg
     1501 agaacgagat cagcttggcg aacagcagcg caccgaacag caccgaatac agcatcaccg
     1561 aggtggtcgc ggtctcgatg aagatgtggc ggaagtcctg ccagctcatg cgccgctgca
     1621 ccatcgagag gatcagcgcc aggcccgcac ccatgcccgc cgcctcggtt gcagtgaaga
     1681 gcttgccgta gaggccgccg atgatgagca cgaagagcag cgtgaagggc cacaccccgc
     1741 gcagcgcgtg cagcttctcg gccatcgtgg tgcgctcgcc gcgcggcgca tcctgcgggc
     1801 tgatgcgggc gaccgcatag atcaccagga ggtacatcag cagcccgagc agcccgggga
     1861 ccacgccagc gacgaacagg tggccgatgt tggtctgggt caggatgccg tagatcatca
     1921 ggatgatcga gggcgggatg aggatgccga gcgtgccgcc ggcggcgatg gtggccgagg
     1981 cgagcgcgtc ggaatagccg tagcgcttca tgctcgggta ggcgaccttc gacatcgtgg
     2041 ccgcggtcgc caggctggag ccgcacaccg cgctgaagcc ggcacaggtg accatggtcg
     2101 acagcgcgag cccgccacgc accggcccga ggaaggcgtt ggcagcgcgg aagaggtcat
     2161 ccgaaatccc ggtacgggcg aggatgttgc ccatcaggat gaacatcggc accacggcca
     2221 gctcgtagga gaacaccgcc tccgagatcg tcatcggcac cagcgaacgc gcgacgtccc
     2281 agcccatgac cacaccgatg ccgccgagcg acaccgacag cagcgcgaag gcgagcggca
     2341 cgcgcaggaa tgccagcacc agcacggcca gcagggaaag aagtccgacg gtcatttggc
     2401 acgctcctgg gtggcgtggg actggacggt gacgggatgc gagggcggcg cggcgaaaca
     2461 gcccagcaac gcggccaggg ccgacagcag cgaggtgaag gcgatgaagc catacatgcc
     2521 gccgcgcggg atcgacagcg actggctgat ctcctggatc ttgccggcct cgatggccga
     2581 gttcaggaac tcgtaggcga tcagcagcag caccagcgcc accagcgccg acaccagccg
     2641 gtgccaccac acccagcccg ggcgtagcag ccacttgtcg aacagatcga cggtgaggtg
     2701 gccgcgcgcc gcggtaagca gcggcaggcc gcagaacacc atgatcgcca tcagatgctc
     2761 ggtggcgtcg tgcgcgccgt agagcggcgc gccgaacagg cggcggccga tgacgtcgat
     2821 gaaggtcagc agcaccatgc ccgccagggt caggatgagc agcgcctcga cgcccttgcg
     2881 gatgcgggac agatacgcgt ccatgtgtct ctccatgtgg gagtggccgg tccgggccgg
     2941 ccacctccgg gatcaagcag cggtcacttg ccggcgtggg ccttgtagcg ctgctcgaag
     3001 aactcgagca tctccatcgg cttgtcgaca ccgaagctcg ggccttcggc cgcccagtcg
     3061 gcgagcatgc gctcgcgcac cgcgccgatg cgctcgaaca gcgccttcga gggcgcgttg
     3121 aagacatggc cctcggcgcg cagcttggcc tcgccggcct tgttctgcgc atcgaagcgc
     3181 tggccccaca ggcgcgacag cttctcgccg gagacgctca tgatcgcctt gcggtcggcc
     3241 tcggagagct tgttccactt gccctcgttg atgacgacga agaagctcgc gtcgtagaag
     3301 ccgcccggga cgatggtgtg atgcttgagc ttgtcctcga cgcggaagtt gatgaccgac
     3361 tcgatggtgt gaagcgacgc atcgaccacc ccgctctcga gcagctcgta ggccttcggg
     3421 ccggggccgg cgaccgccac cgcgcccaga tcctcgagca ggcgcttctg gatcgggccg
     3481 cccatgcgca ccttctggtt cttgaggtcg tcgggctcga cgatgttctt gctgccgtgg
     3541 tgaatctggc cgccgccgaa gagcccgacg ccgagcatga ccacgccctc gaaggccggc
     3601 ttgccggcga ggtatttctc gtaggtctcc cacagcgcca ccgaggacgc ctcggctttg
     3661 gtgccgatga agggcatctc cgagaaccac accgccttga agcgatccgg ttcgtaggtg
     3721 aagttgcccc aggtgatgtc ggccacgccc ttgcgcgcca gctcccagtg ctgggcagga
     3781 gcgcccaccg gcttgggcag gatgcggacg tcgacgcggc cttcggtgac gcgcttgacg
     3841 tcctcgatcc agggctggaa gacctcgggg gtgaggaagt gggtgggcga cacccagctc
     3901 gacatggtca gggtggtggc ggcctgggcg gcgggaagac cggcagcggt ggcgaggacg
     3961 agtccggcgg acagggtggc gaacagcttg ctgagtttca tggtgtctcc ttgcaggatc
     4021 tagttatgga agtactgctc gaccaggccg atccacagcc gggcacccac cgcgaggcag
     4081 gcatcgttga aatcgtagtg cgggttgtgg accatgcatt cgcccgacca ccggccctcg
     4141 gcgtggccgt tgtcgccgtt gccgaccagc aggtagcaac ccggcacctg ctcgagcagg
     4201 aaggcgaaat cctcgctgcc ggtgagcggc tgggcatcgg ccaccacgtg ctcggcgcca
     4261 aaggtctggc gcgcgacctc gaccgcaaag gcggtgggcc gggcggcgtt gaccagcacc
     4321 gggtagccgc gctcgtagcg gatctccgtc ttgcagccat aggcacgcgc ctggttttcg
     4381 gcgagctcgc ggatgcggcg ctcgaccagg tcgcggacct cgggatcgag ggtgcgcacg
     4441 ctcaactcga gctcggcgga ttcggcgatg acgttgtagg ccgcgcccgc ctggaggcgg
     4501 ccgacgctga tcaccgcggc gaggttggga ttgacgttgc gcccgacgat ggattgcagc
     4561 ccgagcaccg tcgccgccgc cggcagggtc ggatcgaccg ccatgtgcgg catgccgccg
     4621 tgcccgccgc ggccgattaa gcggatcagc accttgtcgg acgaggccat gaaggcgccc
     4681 gggcgaacat ggacctggcc gaccgggatg ccgggaaagt tgtgcatgcc gaagatcgca
     4741 tcgcagggga agcgttcgaa gaggccgtct tcgatcatgc gccgcgcgcc gccgccgccg
     4801 ccgagctcct cggccggctg gaagatcagc accagcgtgc cgtcaccctg cagtgccccg
     4861 cccttcaccc gtgcggcgag cgcccgcgcc gcgccgagca gcatggcggt atggccgtcg
     4921 tggccgcagc cgtgcatctt gcccggaacg atgctggccc acggcaggcc ggtcatctcc
     4981 tgcaccggga tggcgtccat gtcggcacgc agcccgatcg tgcgcccgct tccatcgccc
     5041 acccgcagca cccccaccac gccggtgacg gcgatgccgg tgtgcacctc gtagccccag
     5101 gcgcgcagct gctcggcgac gagcgcgctg gtggcgtgct cctcgaagcc cagctcgggg
     5161 ttgcgatgga tggcatggcg ccaccgcttc atctgctccg catcgatctc gtgtgcgact
     5221 gcttcctggg tggtcatccg gctcaccata gatttagctc atgtccagat aataggaact
     5281 tgcaccgaac accgtaaggc gatatttttt cctggatgac aactttcaga ggtcacatga
     5341 accatgaagg accaccagct caaggcactc gtgcaggtcg ccgaaagcgg ttcgatccgc
     5401 gccgcggccc gcgccatgaa cctgagccag agcgcgctga ccaaggcgct gcgcgagctc
     5461 gaagaggacg tcggtgcgga gctgctgcag cgcagctaca agggcatcgg cttcacgccg
     5521 gagggcagcg cgctgctcgt gcgcgcccgc ctggtgctgg cgacgatcga caaggcgcgc
     5581 gcggagatcc gccagatgcg ggggggtgcg ggcgcgcatg taaaggcggc gatcacgccg
     5641 ctgctgacgg cgacggtgct gccggccgtg ctttccgcgt tccgcaaggc acagccggac
     5701 gccgagctga ccttccacga gggcttgctg gtgcaggcct tgcccgggct gatcgagggc
     5761 aagatcgact tcgcgatcgc gctcgcctct ccgcaggacc tgccctacga gatcgagttc
     5821 gaacccttgg ccgacatcga cgcggtaccg atggtgcgcg ccacgcaccc gctggccgaa
     5881 gcgcgcgact ggaacgcggt ggccgacgcc gaatgggtgc tcaacctctc cgccggcagc
     5941 cagagcatga acctggtcgg gtggctgcgc gcccggggtt tccgggcgcc gacccgcatc
     6001 ctgcactgct cctcgcccta cctcatcctc gacatggtcc gccgcaacga cctcatcggc
     6061 tacggcccga aggtactggt gaccgatgct caggtcggcg tcggcctgcg cccgctcgca
     6121 ctgcagccgc tgccgcccac catgccgctc ggcctgatga ccctgcgtgg cgtgccgctc
     6181 ggcagcgcgg cgaagaggct cgcgaacctg tgccgccgag agctgcagtc ctgcctgccg
     6241 cgcccctgat tccctgcctc actcttcccc gcgttcttcc cctcgttctt cccctcgttc
     6301 ttcccctcgt tcttcccctc gttcttccca ataccccggc cggttgtagc tcgccctgag
     6361 gtgctcgatg aaacgccgca ccttcgccgg cagatgacgc tgctgcggat acaccgcctg
     6421 gatgtcgtag ctcggcaccg cgaactcgtc gagtacggtg accagttcgc cgcgcttcaa
     6481 ctcggcctgg atctcccagg tcgagcgcca gcccacgccc agaccttgct tgacccagcc
     6541 gtagagcagt tcgccatcgt tgcaggccag gtcgccctcg acacgaacgg cgaacaacct
     6601 gccgtcacgc aggaaggacc agccgcgctg ctgcccgccg gcgaggttga aggcgaggca
     6661 gttgtggcgg gcgaggtcct cgggctgacg cgggatgccg tggcgctcga agtagtcggg
     6721 cgtgccgcac accacgcgac ggttggggaa caggcgcacg gcgacgtagt tggggtcggt
     6781 cacctcgccg atgcggatcg ccatgtcgta accctcgcgc acgaggtcga ccacgctgtc
     6841 ggtgaagttg aaggacatgc gcagctcggg gtgcagggcc ttgaaggcgg tcgcgtgcgg
     6901 ggccacgtgg cggcggccga aggcggcggg cgcggacacc accagatggc cgcgcaccag
     6961 gtcgcgcccg gcgccgaccg cgcgctcgag ttcgtcgaac tcgcgcagca gcgcgcggct
     7021 ctggtcgagc agttgctcgc caagctcggt cagcgccagg ccccgcgtcg agcggtgcat
     7081 cagcttgacc ccgaggcgtt tctccagcgc gtcgagccgc cgccccatga ccaccggggt
     7141 cacgccctcc accagcgccg cggccgcgaa gctgcccttc tcggccacca gcacgaagct
     7201 gcgcagttcg gtgtagcgat ccatttcctt ctccagggtc gaagggcgcc ggctcgaccg
     7261 ccggattgcg acttgcgtcg ctcctacagc gcggcgcccg ggggccttgc gcgcgggcgg
     7321 taggagcgac gcaagtcgcg attctcggcg gcgacccgtc tcggccatat ttccagtacc
     7381 gatactgcac ataagcgggc gtggatatcg gcccgaggaa gccatcaaac ctgcaactcg
     7441 atactttaag tatctacaga accgactgcc agcccaattc aagcggccct tcccgctccg
     7501 tatgctgcgt ccatcggttt caaggacatc caccccaagg agacagaaga tggcccgcat
     7561 gagagcagtc gacgccgccg ccgcggtgat gcgcaaggaa ggcgtgagca ccgtgttcgg
     7621 cgtgcccggc gccgcgatca acccgctgta ctcggcgatg aagaaggacg gcggcttcag
     7681 gcacatcctc ggtcgccacg tcgagggcgc ctcgcacatg gccgaaggct acacccgcgc
     7741 caacccgggc aacatcggcg tgtgcatcgg tacctcgggc ccggccggca cggacatgat
     7801 caccggcctg tattcggcct ccgccgactc cgtccccatc ctgtgcatca ccgggcaggc
     7861 gccgcgcgcg cgcctccaca aggaggactt ccaggccgtc gacatcgagt ccatcgccaa
     7921 gccggtgacc aagtgggcgg tgaccgtgcg cgagccggcg ctggtgccgc gcgtgttcca
     7981 gcaggccttc cacctcatgc gctccggccg tcccggcccg gtgctgatcg acctgccctt
     8041 cgacgtgcag atggccgaga tcgagttcga catcgacacc tacgaatcgc tcggcgccta
     8101 caagcccgcc gccacccgcg cccaggccga gaaggcgatg gccatgctga atgccgccga
     8161 gcgcccgctg atcgtcgccg gcggcggcgt gatcaacgcc gacgccgcag acagattgca
     8221 ggaattcgcc gagctcgtcg gcgtgccggt gatcccgacc ctgatgggct ggggcgcgat
     8281 ccccgacgac cacccgctga tggccggcat ggcgggtctg cagacctcgc accgctacgg
     8341 caacgccacc atgctggcat cggacttcgt gttcggcatc ggcaaccgct gggccaaccg
     8401 ccacaccggc tcggtggagg tgtacaccag gggacgcaag ttcgtgcatg tggacatcga
     8461 gcccacccag atcggccgcg tgttcggccc ggactacggc atcgtctccg atgcgggcgc
     8521 ggcgctcgac cgcctgctcg aggtggcgca cgggatgaag gccgcaggca ccctgcccga
     8581 tcgcggtgcc tgggtcgccg agtgccagca gcgcaagcgc acgctgcagc gcaagacgca
     8641 cttcgaccaa gtgccgatga agccccagcg ggtgtatgag gagatgaacc tcgccttcgg
     8701 caaggacgcc tgctacgtca gcacgatcgg cctgtcgcag atcgccgccg cgcagttcct
     8761 gcatgtgtac aagccgcgcc actggatcaa ctgcggccag gccggcccgc tcggctggac
     8821 catccccgcg gcgctgggtg tgtgcgcggc cgacccgggt cgcaaggtcg tggcgatctc
     8881 cggcgactac gacttccagt tcatgatcga ggagctggcg gtgggcgcgc agttcaagct
     8941 gccctacctc cacgtgctgg tgaacaatgc ctacctcggc ctgatccgcc agtcgcagcg
     9001 cggcttcgac atggatttct gcgtgcagct cgccttcgag aacatcaacg cccccgagac
     9061 cgaaggctac ggcgtcgatc acgtcggcgt ggtcgagggc ctgggctgca aggcgatccg
     9121 cgtgcgcacg ccggaggaga tccagcccgc cttcgcacag gcgaagcagt ggatggaaga
     9181 attccgcgtg ccggtggtgg tcgagatcat cctcgagcgc gtcaccaaca tcgccatggg
     9241 gaccgagatc gacgccatca acgagttcga ggaactcgcc gaacgtggcg aggacgcccc
     9301 gaccgcgatc tcgatgctcg actgaacgca ccgccccgag gacggggccg tcacggcaca
     9361 ggcaggccgc cccgtccgca accgacaacc ggagacgaag caatgcccaa gttcaacgcc
     9421 aacctgacca tgctcttcaa cgaagtgccc ttcctcgacc gcttccaggc ggcggccgaa
     9481 gccggcttca agggcgtgga gttcctcttc ccctacgcct tcgacaagga cgcgctcgcc
     9541 gaacgcctgg ccaggcacgg cctcgtgcag gtgctgcaca atctgccggc gggcgactgg
     9601 gagggtgggg agcgcggcat cgcctgccat ccggaccgcg tcggcgagtt ccaggacggc
     9661 gtcggacgcg cgatcgaata cgcccgcgcg ctcggctgca agcaggtgaa ctgcctcgcc
     9721 ggcatcgcgc cggccggcgt ggaggccgac aaggtgcacg cgaccttcgt cgccaacctg
     9781 cgcttcgccg ccgccaggct ggaggaggcc ggcatccgcc tgctggtcga gccgatcaac
     9841 accttcgaca tccccggctt ctacctcaac cgcacggcac aggcgatcgc gctgatcgag
     9901 gaggtcggct cgcccaacct gtggctgcag cacgaccttt accacatgca gcgcatggag
     9961 ggtgagctcg ccaacaccat cgcgaagcac ctgccgaaga tcgcccacat gcagatcgcc
    10021 gacaccccgg gccgccacga gccgggcagc ggcgagatca actacgcctg gctgttccgc
    10081 ttcatcgacc agctcggcta cgagggctgg atcggctgcg aatacaagcc cgccgccggc
    10141 acccgcgaag gactgggctg gatccgggcg ctcgccggct gagcgacctt cgcgccttgc
    10201 ggcgctccta cacggaccga cacggtcttg cgaaagcgcc gtaggagcgg cgcaagccgc
    10261 gaacgggccg tcggtctgcg acgagcggca tcgcggctcg acccgccgca ttcgcgcctt
    10321 gcggcgctcc tacacggacc gacacggtct tgcgaaagcg ccgta
//
LOCUS       DAOQNN010000070        10242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3075_length_10242_cov_11.541965, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000070 DAOQNN010000000
VERSION     DAOQNN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3075_length_10242_cov_11.541965"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PKV97_05905"
     CDS             complement(<1..140)
                     /locus_tag="PKV97_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNB05449.1"
                     /translation="MNLLQQLEQEEMARVSQGKTIPEFGPGDTVIVQVKVKEGNRERL
                     QA"
     gene            complement(238..1008)
                     /gene="trmD"
                     /locus_tag="PKV97_05910"
     CDS             complement(238..1008)
                     /gene="trmD"
                     /locus_tag="PKV97_05910"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNB05450.1"
                     /translation="MITLFPEMFAALTGSGITRRAIDRGLYALGFHNPRDFTSDPHRT
                     VDDRPYGGGPGMVMLAEPLMQAIRAARAAQERALGRSGRVIYLSPQGRRLEQEVVLEL
                     AAEPALVLLCGRYEGVDQRLIDREVDEELSLGDFVLSGGELPAMVLLDAIVRQLPGAL
                     NTAGSVQEDSFADGLLDCPHYTRPERYEDEGVPEVLLSGNHAAIRRWRLKQSLGRTWL
                     RRPDLLAARQLSKTELKLLEEFRQEQAGTQDALEPQRG"
     gene            complement(1107..1625)
                     /gene="rimM"
                     /locus_tag="PKV97_05915"
     CDS             complement(1107..1625)
                     /gene="rimM"
                     /locus_tag="PKV97_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300530.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNB05451.1"
                     /translation="MIVLGRIVAPFGVQGWIKIHPFGDDPASWRNMSHWWLCSDDDAP
                     AEAWKQYTLSACRPHGKGLVAALAEISDRGAAEAIDGFYIAAPREALPKPAEGEYYWG
                     DLVGLAVENEAGEAMGTVAGLISTGAHDVLQVREGEGEEGVERLIPFVAAYVLDVDLA
                     TRRIRVAWQKDW"
     gene            complement(1654..1905)
                     /gene="rpsP"
                     /locus_tag="PKV97_05920"
     CDS             complement(1654..1905)
                     /gene="rpsP"
                     /locus_tag="PKV97_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004884679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNB05452.1"
                     /translation="MVVIRLARGGAKKRPFFNMVVADSRNRRDGRFIERVGFYNPIAS
                     GAEKGLVINAERLAYWQQNGAQLSPTVARLVKQAGTAAA"
     gene            2101..3615
                     /locus_tag="PKV97_05925"
     CDS             2101..3615
                     /locus_tag="PKV97_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05453.1"
                     /translation="MEAPSTPSDAEQSLLRWLDSEPADAVEDDLAALRARMHDAFGGT
                     AAGPATSDALLCALAERMGDIGGRLRPLLLARQLPVDVPLFEANEQLCVLALDVATAV
                     SAAAGRAGRARPGGGAELCEYAIGVVTEIFVLSALAAAAPPAGTWRLAHDFAAHHEVA
                     AAAADDPLRCAYLRLLAVAVAQPEGLTGREIAWLYDFLDARVLGAAVLARGTGDTSAS
                     AWWIDLGADLGPQSVARRPAPSESVLGGDLWSFNPRPMAQKLGECIEWVTARMTAAEL
                     AGMQGEVESLDAEEALFPPGLSPLETVALLRRLRSHWMAAPVRGQPRRQHQYVVQVGI
                     GLQSVWDLGSGGRDDPALLQEWMVLNESPGGLAIMSVAGVRGEIEAGGALALRRKPEQ
                     AWSVCIVRWVRSDCPGQVEVGLQIIGQSFHPVMVGFRGRAVQQLSPALALPIMEPLRR
                     HPAMLAVAGTYASRRFVFVRGTQTVYVAQARALGLDLQTPSVELFQYEIDPYPI"
     gene            3702..4301
                     /gene="wrbA"
                     /locus_tag="PKV97_05930"
     CDS             3702..4301
                     /gene="wrbA"
                     /locus_tag="PKV97_05930"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNB05454.1"
                     /translation="MHEILVLYYSHRGSVRALAEHIAAGIHQVPGAVARVRTVPRVST
                     VCEAVEDDIPASGPPYVEVADLEECVGLALGSPTRFGNMAAPMKYFLDGLAGAWLNGA
                     LEGKPGCVFTSSSSLHGGQESTLLSMMLPLFHHGMLVLGLPFSEPALGETRSGGTPYG
                     ASHVSGSEGDSAPSAEEIALAQAQGRRLAEVAMRLAGSR"
     gene            4298..4645
                     /locus_tag="PKV97_05935"
     CDS             4298..4645
                     /locus_tag="PKV97_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HNB05455.1"
                     /translation="MNARMLSVVSSVALLALIALCVIWEGWLAPLRPGGSWMMLKALP
                     LLPAVFGVLRGRRYTSQWLSMLSLLYFTEGVLRATDPGLTGVFALVEVFLAVVLFTST
                     MLHARATAPSRAQ"
     gene            complement(4707..6242)
                     /locus_tag="PKV97_05940"
     CDS             complement(4707..6242)
                     /locus_tag="PKV97_05940"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNB05456.1"
                     /translation="MKDKLIIFDTTLRDGEQSPGASMTRDEKLRIARQLERMRVDVIE
                     AGFAAASNGDFEAVRSIAEAIKESTVCSLARANEHDIRRAGEAIRPAARGRIHTFIAT
                     SPIHMEKKLRMTPDQVVEQAVKAIGWAREYTDDVEFSAEDAGRSEIDFLVRIFEAVID
                     AGAKTINVPDTVGYNVPEQFAATVRTLIERVPNSDKVVWSVHCHNDLGLAVANSLAAV
                     RTGARQVECTINGLGERAGNASLEEIVMAVRTRADIFPVETGVDTTQIVPASKLVSQV
                     TGYPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWGANKLVLGKLSGR
                     NAFRARLQELGIEIESEEHLNQAFARFKELADRKSEIFDEDLTALMRDEAVTPAPEQY
                     RLVSTRFHSETGETPVAEITLSIGGAEKHARANGSGPVDAAFKAIETVATTGSELLLY
                     SVNAITSGTDAQGEVTVRLSRDGRVMDGQGADTDIIVASAKAYLNALNKLHVGAERLN
                     PQV"
     gene            complement(6239..7129)
                     /gene="pssA"
                     /locus_tag="PKV97_05945"
     CDS             complement(6239..7129)
                     /gene="pssA"
                     /locus_tag="PKV97_05945"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNB05457.1"
                     /translation="MTILESTEATPKVPDNEHAPLPLSDKRRRGIYILPNLFTTAALF
                     AGFYAIVQAMNQRFEYAAVAIFVAMVLDGLDGRIARLTNTQSEFGAEYDSLSDMVSFG
                     AAPALVMYEWALQDLGKLGWVAAFIYCAGAALRLARFNTNIDVVDKRYFQGLPSPAAA
                     ALVAGMVWVGIDNGFAGNELRWYAAVLTIFAGVSMVSNVLFWSGKSINLRKSVPFIVL
                     IALVLAFALISSYPPGILFGLFLAYAGSGYVVAALRWRRRRTHRAEPAAPQAAAYTAQ
                     AGDAAAAPVEGASSDPGSKP"
     gene            complement(7286..8302)
                     /gene="ilvC"
                     /locus_tag="PKV97_05950"
     CDS             complement(7286..8302)
                     /gene="ilvC"
                     /locus_tag="PKV97_05950"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNB05458.1"
                     /translation="MKVYYDKDADLSLIKGKQVTIVGYGSQGHAHAQNLRDSGVNVTV
                     GLRKDGSSWAKAEAAGLAVKEIGEAVKAADLVMILLPDETAPQVYRDDIEPNIKKGAV
                     LAFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYLRGGGVPTLIAIYQDRSGKA
                     KDIALSYSAANGGTKGGVIETNFREETETDLFGEQAVLCGGAVELVKMGFETLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPEVINEQSRAAMRN
                     ALKRIQTGEYAKMFILEGKTNYPSMTAYRRLNADHQIEKVGGQLRDMMPWIKAKALVD
                     KSKN"
     gene            complement(8344..8835)
                     /gene="ilvN"
                     /locus_tag="PKV97_05955"
     CDS             complement(8344..8835)
                     /gene="ilvN"
                     /locus_tag="PKV97_05955"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNB05459.1"
                     /translation="MRHVISLLIENESGALSRVSGLFSARGYNIESLTVAPTEDFSMS
                     RMTIVTTGSDEIIEQITKQLNKLVEVVKVVDISEAAHIERELMLVKVRATGKDREEIK
                     RTADIFRARIIDVTDASYVVELTGNQGKLDAFIGAIDPALILETVRSGVCGIGRGDRV
                     LKV"
     gene            complement(8867..>10242)
                     /gene="ilvB"
                     /locus_tag="PKV97_05960"
     CDS             complement(8867..>10242)
                     /gene="ilvB"
                     /locus_tag="PKV97_05960"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNB05460.1"
                     /translation="EVDTVGITRPCVKHNFLVRDVRDIAATMKKAFHLARTGRPGPVL
                     VDIPKDVSMQRCEYEYPKEISMRSYNPVTKGHSGQIKKAVQLLLEAKRPMIYTGGGVV
                     LSNAAEQLVKLTRLLGYPITNTLMGLGGYPSSDRQFLGMPGMHGTFEANMAMHYSDVL
                     LAVGARFDDRVIGNPAHFAEEPRKIIHVDIDPSSISKRVRVDTPIVGDVKEVLDEMIR
                     QIESGEARPNPEALASWWKQIEQWRERKCMVYKNSDEIIKPQFVIQKLWEVTGGDAIV
                     TSDVGQHQMWAAQYYGFDKPRRWLNSGGLGTMGVGMPYAMGAKLANPDMQVACVTGEA
                     SIQMNIQELSTCKQFRLPIKICNLNNRYLGMVRQWQELFHGGRYSESYMDSLPDFAKL
                     AESYGHVGIRVDRPADVEGALREAFTTHKDELVFLDFQIDQTENVYPMVQGGKGLTEM
                     ILSAEDL"
BASE COUNT         1442 a         3299 c         3665 g         1836 t
ORIGIN      
        1 taggcctgca gacgctcgcg gttgccttcc ttgaccttca cctggacgat cacggtgtcg
       61 ccgggaccga actcggggat ggtcttgccc tgcgagacgc gggccatttc ttcctgttcg
      121 agttgctgaa gcaggttcat tgcagatact ccgtcgatgt tatcggcctt cgcctcttgc
      181 gtcggcctgg ccgcagagca ggtcgcaacc tgatgcgcgc cgtttgtgaa aggacgctca
      241 gccgcgctgg ggttccagtg cgtcctgcgt cccggcttgc tcctgcctga actcttcaag
      301 cagtttcaat tcggtcttgc tgagctggcg cgccgcgagc aggtcggggc ggcgcagcca
      361 ggtgcggccg agcgactgct tcaggcgcca gcggcggatt gccgcatggt tgcccgacaa
      421 cagcacctcg ggcacgcctt cgtcttcgta ccgctccggc cgcgtgtagt gcggacagtc
      481 cagcaggccg tcggcgaagg aatcctcctg caccgagccg gcggtgttca gtgcgccggg
      541 cagctggcgc acgatcgcgt cgagcagcac catcgccggc agctcgccgc ccgacagcac
      601 gaagtcgccg agcgaaagct cttcgtcgac ctcgcggtcg atcaggcgct gatccacgcc
      661 ttcgtagcgc ccgcacagca acaccagcgc gggctcggcg gccagttcca gcaccacctc
      721 ctgctccagc ctgcgcccct gcggcgacag gtagatgacc cgcccgctac gtccgagcgc
      781 cctttcctgc gccgcgcgtg cggcgcggat cgcctgcatc agcggctcgg ccagcatcac
      841 catgccgggg ccgccgccgt aggggcggtc gtccaccgtg cgatgcggat cgctggtgaa
      901 gtcgcgcgga ttgtggaacc cgagcgcgta cagcccgcga tcgatcgccc tccgcgtgat
      961 gccgctgccg gtgagcgcgg cgaacatctc ggggaagagg gtgatcacgt cgtatcgacg
     1021 cgggccgcct gcaacgcccg aagggctcgc ctgctgtgtt tcgccttgcg ggctctgcgg
     1081 attcaccggc cttccacagt cccgtgttac cagtctttct gccaggcgac ccggatgcgc
     1141 cgcgtggcca ggtcgacgtc gagcacgtag gcggcgacga aggggatcag gcgttcgacc
     1201 ccctcttcac cttcgccttc acgcacctgc aacacgtcgt gcgcgccggt cgagatcaac
     1261 cctgcgaccg tgcccatcgc ttcacccgcc tcgttctcga ccgccaggcc gacgaggtcg
     1321 ccccagtagt actcgccctc ggcaggcttg ggcagcgcct cgcgcggcgc ggcgatgtag
     1381 aagccgtcga tcgcctcggc ggcgccccgg tcggatatct cggccagtgc ggccaccagc
     1441 cccttgccgt gcggccggca ggcgctcaac gtgtattgct tccacgcctc ggcgggcgcg
     1501 tcgtcgtcgc tgcacagcca ccagtgcgac atattccgcc acgaggcggg gtcgtcaccg
     1561 aagggatgga tcttgatcca gccctgaacg ccaaaagggg cgacgatgcg ccccagtacg
     1621 atcatgcgcc gctcggccgc tgatcgaagt cgatcaggcc gcggccgtgc cggcctgctt
     1681 gaccaggcga gcgacggtgg gcgagagctg ggcgccgttc tgctgccagt aggcgaggcg
     1741 ctcggcgttg atcacgaggc ctttctcggc gcccgaggcg atcgggttgt agaagccgac
     1801 gcgctcgatg aagcgaccgt cgcggcggtt gcgcgaatcg gcaacgacca tgttgaagaa
     1861 cgggcgcttt ttggcgccac cacgggcaag gcgaatgacg accatctgga tgttcctgat
     1921 cggtggatgc gaaaaaccgt ggattatagg ggagtcgccc gtcgcggaca aggttttatt
     1981 gcggccgacg agcgattcct cgacatcttc cgcgtaccgg cgccgggtgt ccgcagtgtt
     2041 gcggcgtgtg tgcgcgggat ggggatagaa tgtgacccga gcaatgtttc tccggggacc
     2101 atggaagcgc cttcgacacc gtccgatgcc gagcagtcgc tgctgcgctg gctcgacagc
     2161 gagcctgcag acgccgtgga ggatgacctc gccgcgctgc gagcgcgcat gcacgacgct
     2221 tttggcggca ccgctgccgg tccggcgacg agcgacgcgt tgttgtgcgc gctggccgag
     2281 cgcatgggcg acatcggtgg ccggctgcgg cccttgctgc tcgcgagaca gctcccggtg
     2341 gacgtgccgc tgttcgaggc gaacgagcag ttgtgcgtgc tcgcgctcga tgtcgccacg
     2401 gccgtctcgg ctgcagccgg gcgcgccgga agggcgcggc cggggggcgg agcggagctc
     2461 tgcgaatacg cgatcggcgt ggtgaccgag atcttcgtgc tgagcgcgct ggcggcggct
     2521 gcgcctcccg cggggacttg gcggttggcg cacgacttcg cggcgcacca tgaggttgcc
     2581 gcggctgccg cggacgatcc cctgcgctgt gcctacctgc gtctgctggc cgtcgcggtc
     2641 gcacagcccg aggggcttac cggccgcgag atcgcctggt tgtacgactt tctcgatgcg
     2701 cgtgttctcg gcgccgcggt gctcgcgcgc ggcaccggcg acacgagcgc atcggcgtgg
     2761 tggatcgacc tcggcgccga cctggggccg caatccgtgg cgcggcggcc ggcgccctcg
     2821 gagtcggtgc tcggcggcga tctgtggagc ttcaatccgc gcccgatggc gcagaagctg
     2881 ggcgagtgca tagagtgggt gacggcgcgc atgaccgcgg cagaactcgc gggcatgcag
     2941 ggcgaagtcg agtcgctgga tgccgaggag gcgctgtttc cgcctggcct ctcacccctc
     3001 gagacggtgg ccttgctgcg ccgccttcgc agccactgga tggcggcgcc ggtgcggggg
     3061 cagccgcggc gccagcatca atacgtggtt caggtcggga tcggcctgca atcggtgtgg
     3121 gatctgggca gtgggggaag ggatgaccct gcgttgctgc aggagtggat ggtcctcaac
     3181 gaaagcccgg gcggccttgc gatcatgagt gtggccggcg tgcgcggcga gatcgaggcg
     3241 ggcggcgcgc tggccctgcg gcgcaagccc gagcaggcct ggtcggtgtg catcgtgcgc
     3301 tgggtgcgca gcgattgccc ggggcaggtg gaggtcgggt tgcagatcat cgggcagtcc
     3361 ttccatccgg tcatggtcgg ctttcgcggg cgtgcggtgc agcagctctc gcccgcgctc
     3421 gcgctgccga tcatggagcc gctgcgccgc catccggcga tgctcgcggt cgccggcacc
     3481 tacgcctcgc gccgcttcgt cttcgtacgc gggacgcaga cggtctatgt cgcccaggca
     3541 cgcgcgctcg ggctcgacct gcagacgccg agcgtcgaac ttttccagta cgagatcgat
     3601 ccctatccga tctgagcgca ttgcccgcgt gcgccttcgg gctgcgggtg gccggcgacg
     3661 ccgcgcgata taatccgctc cccttccgga actggcatcc gatgcacgaa atcctcgtcc
     3721 tctactacag ccaccgcggt tcggtgcgcg cgctcgccga gcacatcgcc gccggcatcc
     3781 atcaggtgcc gggcgcggtg gcgcgggtgc gcacggtgcc gcgggtgtcc accgtgtgcg
     3841 aggcggtgga ggacgacatc ccggcgtccg ggccgcccta cgtcgaggtc gccgacctgg
     3901 aggagtgcgt cggcctggcg ctcggcagcc cgacgcgctt cggcaacatg gcggcgccga
     3961 tgaagtactt cctcgacgga cttgccggtg cctggctcaa cggcgccctc gagggcaagc
     4021 cgggctgcgt gttcacgtcc tcgtccagcc tgcatggcgg gcaggagagc acgctgctgt
     4081 cgatgatgct gccgctcttc catcacggca tgctggtgct cggcctgccg ttctccgagc
     4141 cggcgctcgg cgagacgcgc agcggcggca cgccctatgg cgcgagtcat gtctccggga
     4201 gcgagggcga ttctgcgccg agcgcagagg agatcgcgct cgcgcaggcg cagggccggc
     4261 gccttgccga ggtggcgatg cgcctggcgg ggtcgcgatg aacgcgcgca tgctgagcgt
     4321 ggtgtcgagc gtcgcgctgc tggcgctgat cgcgctgtgc gtgatctggg agggctggct
     4381 cgccccgctg cgtccgggcg gctcgtggat gatgctgaag gcgctgccgc ttctgccggc
     4441 ggtgttcggc gtgctgcgcg gccgccgcta cacctcgcag tggctgtcga tgctgtcgct
     4501 gctgtatttc accgagggtg tgctgcgcgc caccgacccc gggctcaccg gcgtgttcgc
     4561 cctcgtcgag gtcttcctcg cggtcgtgtt gttcaccagc acgatgctgc atgcccgtgc
     4621 gacggctccc tcccgcgcgc agtgaggcgg gcggggcggg gtcggcaagt tccgcacccg
     4681 cccttcgctc ctgacgcctt acgccctcat acctgcgggt tgagccgttc ggctcccacg
     4741 tgcagcttgt tgagcgcgtt caggtaggcc ttggccgagg cgacgatgat gtcggtgtcg
     4801 gcgccctgtc cgtccatcac gcggccgtcg cgcgacaggc gcacggtgac ctcgccctgg
     4861 gcgtcggtgc cgctggtgat cgcgttcacc gagtagagca gcagctcgct tcccgtcgtc
     4921 gccaccgtct cgatcgcctt gaaggcggcg tccaccgggc cggagccgtt ggcacgggcg
     4981 tgcttctcgg cgccgccgat cgacagggtg atctcggcca ccggcgtctc gccggtctcg
     5041 gagtggaagc gggtcgacac caggcggtac tgctcgggcg ccggggtcac cgcctcgtcg
     5101 cgcatcagcg cggtgagatc ctcgtcgaag atctccgact tgcggtcggc gagctccttg
     5161 aagcgcgcga aggcctggtt caggtgctcc tcgctctcga tctcgatgcc gagctcctgc
     5221 aggcgggcgc ggaaggcgtt gcgaccggag agcttgccga gcacgagctt gttggcgccc
     5281 cagccgacgt cctcggcgcg catgatctcg taggtctcgc ggtgcttgag cacgccgtcc
     5341 tggtggatgc cggactcgtg cgcgaaggcg ttggcgccga cgatggcctt gttgggttgc
     5401 accgggtagc cggtgacctg cgaaaccagc ttcgaggccg ggacgatctg cgtggtgtcg
     5461 acgccggtct cgaccgggaa gatgtcggcg cgggtgcgca ccgccatgac gatctcttcg
     5521 agcgatgcgt tgcccgcgcg ctcgcccagg ccattgatgg tgcactcgac ctggcgcgcg
     5581 cccgtgcgca ccgcggcgag cgagttggcc acggccagcc cgaggtcgtt gtggcaatgc
     5641 accgaccaca ccaccttgtc cgagttgggc acgcgctcga tcagcgtgcg cacggtggcg
     5701 gcgaactgct cggggacgtt gtagccgacc gtgtcgggca cgttgatggt cttcgcgccg
     5761 gcgtcgatca cggcctcgaa gatgcgcacc aggaagtcga tctcggagcg gccggcatcc
     5821 tctgcggaaa actcgacgtc gtcggtgtat tcgcgcgccc agccgatcgc cttgaccgcc
     5881 tgctcgacga cctgatcggg cgtcatgcgc agcttcttct ccatgtggat cgggctggtg
     5941 gcgatgaagg tgtggatgcg gccgcgcgcc gccggccgga tggcctcgcc ggcgcggcgg
     6001 atgtcgtgct cgttggcgcg cgccagcgag cacacggtgg attccttgat cgcctcggcg
     6061 atcgagcgca ccgcctcgaa gtcgccattg gaggcggcgg cgaagccggc ctcgatgacg
     6121 tcgacccgca tgcgttcgag ctggcgggcg atgcgcagct tctcgtcgcg cgtcatcgac
     6181 gcgccggggc tctgctcgcc gtcgcgcaag gtggtgtcga agatgatcag cttgtctttc
     6241 atggcttgct cccggggtcg gatgaagcgc cctcgacggg cgcggcggcg gcgtcgccgg
     6301 cttgtgcggt gtacgcggcc gcctggggtg cggcgggttc ggcgcgatgg gtgcggcgcc
     6361 ggcgccagcg cagcgcggcg accacgtagc ccgagccggc gtaggcgagg aagaggccga
     6421 agaggatgcc gggcgggtag ctggagatga gcgcgaaggc gagcaccagc gcgatcagca
     6481 cgatgaaggg cacgctcttg cgcaggttga tgctcttgcc gctccagaac agcacgttgc
     6541 tgaccatgga gacgccggcg aagatggtca gcaccgcggc gtaccagcgc agctcgttgc
     6601 cggcgaagcc gttgtcgatg cccacccaca ccatgccggc gaccagggcc gccgccgcgg
     6661 gactgggcag gccctggaag tagcgcttgt cgaccacgtc gatgttggtg ttgaagcgtg
     6721 ccaggcgcag cgcggccccg gcgcagtaga tgaaggcggc gacccagccg agcttgccga
     6781 ggtcctgcag cgcccactcg tacatcacca gcgccggtgc ggcgccgaag gagaccatgt
     6841 cggagagcga gtcgtactcg gcgccgaact cgctctgggt gttggtgagg cgggcaatgc
     6901 ggccgtcgag gccgtcgagc accatggcga cgaagatcgc caccgcggcg tactcgaagc
     6961 gctggttcat cgcctgcacg atcgcataga agccggcgaa cagcgctgcg gtggtgaaca
     7021 ggttcggcag gatgtagatg ccgcggcggc gcttgtcgga caagggcagc ggggcgtgtt
     7081 cgttatcggg caccttgggc gtcgcctccg tggattccag gatcgtcatt ttagcgtacg
     7141 cgcgcgccgt gccggctgca aaagcgcccg cgccgccgca tgacgatcct gcggtgcgtg
     7201 cgcttgcgcg ggcgatcggg cgcagcaatg aaaaaggccg gcaccgcgag tgcggtaccg
     7261 gcccttggac cgaacgccgg ggcgatcagt tcttcgactt gtcgaccagg gccttggcct
     7321 tgatccacgg catcatgtcg cgcagctggc caccgacctt ctcgatctgg tggtcggcgt
     7381 tgaggcggcg gtaggcggtc atgctcgggt agttggtctt gccctcgagg atgaacatct
     7441 tggcgtactc gccggtctgg atacgcttga gcgcgttgcg catggcagcg cgcgactgct
     7501 cgttgatgac ctcgggcccg gtcacgtact cgccgtactc ggcgttgttc gagatcgagt
     7561 agttcatgtt ggcgatgccg ccctcgtaca tcaggtcgac gatcagcttg agctcgtgca
     7621 ggcactcgaa gtaggccatc tcgggggcgt agccggcttc caccagggtc tcgaagccca
     7681 tcttgaccag ctcgacggcg ccgccgcaca gcacggcctg ctcgccgaag aggtcggtct
     7741 cggtctcttc gcggaagttg gtctcgatca cgccgccctt ggtgccgccg ttggcagcgg
     7801 agtacgacag ggcgatgtcc ttggccttgc ccgagcggtc ctggtagatc gcgatcaggg
     7861 tcggcacgcc gcccccgcgc aggtactcgg agcgcacggt gtggccgggg cccttggggg
     7921 cgaccatgat gacgtcgaga tcggcacgcg gcacgatctg gttgtagtgc acgttgaagc
     7981 cgtgggcgaa ggcgagcacg gcgcccttct tgatgttcgg ctcgatgtcg tcgcgataga
     8041 cctggggcgc ggtctcgtcc ggcagcagga tcatgacgag gtcggcggcc ttgacggctt
     8101 cgccgatttc cttgacggcc aggccggcgg cctcggcctt ggcccacgag ctgccgtcct
     8161 tgcgcaggcc gacggtgaca ttcacgccgg agtcgcgcag gttctgggca tgggcgtggc
     8221 cctgcgagcc gtagccgacg atggtgacct gcttgccctt gatcagcgag aggtcggcgt
     8281 ccttgtcgta gtaaaccttc atgtctttcc tttggttcga tgtggagcgg gggagggggg
     8341 agctcagacc ttgagcacgc ggtcgccgcg gccgatgccg cagacgcccg agcgcacggt
     8401 ctcgaggatc agtgccgggt cgatcgcgcc gatgaaggcg tcgagcttgc cctggttgcc
     8461 ggtgagttcg acgacgtagg aggcgtcggt gacgtcgatg atgcgggcgc ggaagatgtc
     8521 ggcggtgcgc ttgatctcct cgcggtcctt gcccgtggcg cgcaccttga ccagcatgag
     8581 ttcgcgctcg atgtgggcgg cctcggagat gtcgacgacc ttgacgacct cgaccagttt
     8641 gttcagctgc ttggtgatct gctcgatgat ctcgtcggaa ccggtggtga cgatggtcat
     8701 ccgcgacatc gagaaatcct cggtcggagc cacggtcagc gattcgatgt tgtagccgcg
     8761 cgccgagaac aggccggaca cgcgcgacag cgcgccggat tcgttttcga tcaggaggga
     8821 aatgacgtga cgcatggtga tgtgttccgg attcggtagg aggcgatcaa aggtcttcgg
     8881 ccgacaggat catctcggtg agacccttcc cgccctgcac catcggatag acgttttcgg
     8941 tctggtcgat ctggaaatcg aggaacacga gctcgtcctt gtgggtggtg aaggcctcgc
     9001 gcagcgcacc ctcgacgtcg gccggccggt cgacacggat gccgacgtgg ccgtaggact
     9061 cggccagctt ggcgaagtcg ggcagggagt ccatgtagga ctcggagtag cgcccgccgt
     9121 ggaacagctc ctgccactgg cgcaccatgc ccaggtagcg gttgttgagg ttgcagatct
     9181 tgatcggcag gcggaactgc ttgcaggtcg agagctcctg gatgttcatc tggatcgaag
     9241 cctcgccggt cacgcaggcc acctgcatgt cggggttggc gagcttggcg cccatggcat
     9301 agggcatgcc cacgcccatc gtgcccaggc cgcccgagtt gagccagcgc cgcggcttgt
     9361 cgaagccgta gtactgcgcc gcccacatct ggtgctggcc gacgtcggag gtgacgatgg
     9421 cgtcgccgcc ggtgacttcc cacagcttct ggatcacgaa ctgcggcttg atgatctcgt
     9481 ccgagttctt gtagaccatg cacttgcgct cgcgccactg ctcgatctgc ttccaccacg
     9541 aggccagcgc ctcggggttg gggcgcgcct cgccggattc gatctggcgg atcatctcgt
     9601 cgagcacttc cttgacgtcg ccgacgatcg gagtgtcgac gcgcacgcgc ttggagatcg
     9661 acgaggggtc gatgtccaca tggatgatct tgcgcggctc ttcggcgaag tgcgccggat
     9721 tgccgatcac gcggtcgtcg aagcgggcgc cgacggcgag cagcacgtcg gagtagtgca
     9781 tcgccatgtt ggcttcaaag gtgccgtgca tgcccggcat gccgaggaac tggcggtcgc
     9841 tcgaggggta gccgccgagg cccatgaggg tgttggtgat cgggtagccg aggagccgcg
     9901 tgagcttgac gagctgctcg gccgcgttgg agagcaccac gccgccgccg gtgtagatca
     9961 tcgggcgctt ggcttcgagc agcagctgca ccgccttctt gatctggccc gagtggccct
    10021 tggtcaccgg gttgtacgag cgcatcgaga tctctttcgg gtactcgtac tcgcagcgct
    10081 gcatcgacac gtccttcggg atgtcgacca ggaccgggcc ggggcggccg gtgcgggcga
    10141 ggtggaaggc cttcttcatc gtcgcggcga tgtcgcgcac gtccctgacg aggaagttgt
    10201 gcttgacgca cgggcgggtg atgccgaccg tgtcgacttc ct
//
LOCUS       DAOQNN010000071        10179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3102_length_10179_cov_12.445279, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000071 DAOQNN010000000
VERSION     DAOQNN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3102_length_10179_cov_12.445279"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PKV97_05965"
     CDS             <1..926
                     /locus_tag="PKV97_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB05461.1"
                     /translation="NVSTKLAGRIEEMLVDEGDFVEAGQALVRMQTDVLVAQRAEAVA
                     QLQQARQAVATATAQMAMREADLAASQALIAQRESELSYSRRRLTRYELAGEKSATSV
                     QEIDEERTRSLVAEATLTAAKAQATAAQAAIEAARAEIAGAESTVHAREATVQRIEAD
                     IKDSVLASPRAGRVQYRIAQPGEVLAAGGRALNVIDLADVYMTFFLPEQVAGRVAMGT
                     EVRLVLDAAPGFVIPAEVSFIASTAQFTPKTVETAEERQKLMFRVRARISPDLLRKHL
                     HQVKTGVPGVAWIKLDPAAHWPADLTVKVPE"
     gene            930..3701
                     /gene="rbbA"
                     /locus_tag="PKV97_05970"
     CDS             930..3701
                     /gene="rbbA"
                     /locus_tag="PKV97_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HNB05462.1"
                     /translation="MNDASPSSSVARLHDVALRYGRTIALSGVSLDIPARTMVGLIGP
                     DGVGKSTLLSLIAGARRVQQGMVEVLGGDICRSSHRRDVCTRIAYMPQGLGKNLYPTL
                     SIEENLQFFARLFGQDSAERRRRIDDLTRSTGLFRFLDRPAGKLSGGMKQKLGLCCSL
                     IHDPDLLILDEPTTGVDPLARRQFWDLIAHLRQDRPEMSVVVATAYMEEAERFDWLVS
                     MDEGKVLATGTPTELHERTGTDSLEAAFIELLPEEKRRGHKPVQIAPFVGGEADEIAI
                     ESRGLTKRFGNFVAVDHVTMRVRRGEIFGFLGSNGCGKTTTMKMLTGLLPASEGEAEL
                     FGRPLDAGDLATRGRVGYMSQSFSLYKELTVRQNLVLHARIFSLPAGEIPGRVAEMGE
                     RFGLTRVMDALPDNLPLGMRQRLSLAVAMVHGPELLILDEPTSGVDPIARDSLWALMI
                     ELSRRDRVTIFISTHFMNEAERCDRISFMHAGKVLVSDAPADIVRERGVASLEAAFIA
                     YLVDAGAETTHAGAAAAATPRTAPATDELPTSRTAVHRSDRSMFSVQRALSYSLREAL
                     ELIRDPVRGALALLGSIVLLLVIGYGITMDVENLSYAVLDRDQTVLSRDYTINLSGSR
                     YFTQRPPLADYAELDHRMRTGELSLAIEIPPGFGRDIERGDPVEIGAWADGAMPQRAE
                     TVRGYVQGMHQLWLQEQYRRRGIDVAANATVESRYRYNPDVRSLPAMVPAIVPLMLLM
                     IPAMLAALSVVREKELGSIINFYATPVTRAEFLLGKQLPYIALGVVNFLLMAALAVTI
                     FGVPMKGSFWVLLLASGLFVGFATGFGLFVSTLVRGQIAAIFATMIATMIPTIQYAGL
                     INPVASLEGIGAAIGRVYPATHMIIVSRGVFNKGLRLHDLHDALWAIGLSVPVILIAA
                     VAMLRKQER"
     gene            3701..4831
                     /locus_tag="PKV97_05975"
     CDS             3701..4831
                     /locus_tag="PKV97_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB05463.1"
                     /translation="MKQHPLVIIAHLGVKELRGLLRDPAMLFLIVFAFTAAIYTAGTA
                     MPESMHNASIAIVDEDRSALSSRIIAALQPPYFTPPRHVWLDEADAGLDAGRFTFVLN
                     IPPNFQRDVLAGKAPAVQLNVDATRMSQAFSGNGYVQQIVQAEVDEFVSGVRARPRTP
                     VELALRMRFNPNLQQSWFGSLMEIINMVTMLSLILTGAALIREREHGTIEHLLVMPVT
                     PAQIMTAKVWSMGLVVLVGAAFSLTVMVQRVLHVTVHGSVALWLLAAALHLVATTSMG
                     IFMATVARSMPQFGLLVVLTIIPLQLLSGGTTPRESMPEAIRTLMLAAPTTHFVSASQ
                     AILYRGAGLEIVWPQLATVLGIGTVFFTIALRRFRGTLNRMA"
     assembly_gap    5082..5181
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5182..5935
                     /locus_tag="PKV97_05980"
     CDS             <5182..5935
                     /locus_tag="PKV97_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05464.1"
                     /translation="DKEVLHAIAVEHHKGRRLLIATTNLDANRPVIWDLGAVAASGSP
                     QALDLFRDIIMASAAVPVLFPPVYIDVECDGIVYDEMHVDGGVTSQVLLYGSAVSRHD
                     VVRESSPNAPSATYYVIRNGKFATDWAPVRPRVLDIASRSISRIIQAQGVGDLWQVYA
                     ACRRDGLEFRLAFIPDEIALPTDPEFDTGVMADLFERGRTLAREGYPWRTEPPGLRSN
                     GPYDEARPGPSGSGRGPTNPLCRAVYQHAGRP"
     gene            5932..6390
                     /locus_tag="PKV97_05985"
     CDS             5932..6390
                     /locus_tag="PKV97_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05465.1"
                     /translation="MMNPSTHEPVAKPDPALVVAAFTQTSENIRHIRSERIWFANAYG
                     AIVAGGLALLPRDLSIVSNRRVVLASLVVLVLFSGVALACSIRLVAELRTALANLARI
                     ASDNGMDRLVGDIDTPRSFGARMPMRWVFPIYFSITTVCLLVLLISHLLA"
     gene            6408..7202
                     /locus_tag="PKV97_05990"
     CDS             6408..7202
                     /locus_tag="PKV97_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05466.1"
                     /translation="MSNGQRGDAPQESRLVESIKSPHAQPSPALAVTRRWSVVVVLVS
                     VMAALLVYAALPRDMGELGRRAVGIAVFAAALWATEAIPLFPAWLSIVGLDVLLLSRG
                     GERRAGGHGQPQLHPVLRAVRVEHGHPVPKWVPVDRGGAEARAGPRDRLAVGAAVRRR
                     LYRLVMAVILTTGFLSIWISNTATAAMMLAIVTPLARNPATPRRLGSRHAAVTLFAAA
                     DAIRCLASIPSASDVHHRRDAKPPRRLESAHDAPNDTKVHSWSYHS"
     gene            7153..>8132
                     /locus_tag="PKV97_05995"
     CDS             7153..>8132
                     /locus_tag="PKV97_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HNB05467.1"
                     /translation="MTRPMTPKSTHGHTIRRSASGGAQSGPLSSRDLELIDAYWRASN
                     YLSVGQIYLLDNPLLRQPLKREHVKPRLLGHWGTTPGLNFIYVHLNRVIKRDDLSVLY
                     IAGPGHGGPALVANVYLEGTYSEVYPNISQDEEGMKRLFVQFSFPGGIPSHVAPETPG
                     SIHEGGELGYALSHAFGAAFDNPDLIVACVVGDGEAETGALATSWHSNKFLNPVTDGA
                     VLPILHLNGYKIANPCVLARIPHEELDALFRGYGYAPIYVEGHEPEKMHQLMAAAMDK
                     AFADIRAIQNDTRAKGFKQRPTWPMIVLRSPKGWTCPAEIDGKKCEDYWRS"
     assembly_gap    8133..8232
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8233..8880
                     /locus_tag="PKV97_06000"
     CDS             <8233..8880
                     /locus_tag="PKV97_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HNB05468.1"
                     /translation="PQWLTMDQAVKHCEAGLGIWEWASNDMGHEPDVVMACCGDVPTL
                     ETLAAVDLLREHFPEIRIRVVNVVDLMTLQSPSDHPHGLSDHDFDTLFTKDKPIVFAF
                     HGYPWLIHRLTYRRTNHENLHVRGYKEEGTTTTPFDMCVLNELDRFHLVGDVIDRVPA
                     LGARAAYVKQRIRNALIDHREYITRRGEDDPRIVGWKWGGGKTASTRSATEGDNV"
     gene            8887..10071
                     /locus_tag="PKV97_06005"
     CDS             8887..10071
                     /locus_tag="PKV97_06005"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNB05469.1"
                     /translation="MNDALLILNAGSSSVKFSVFDSPGLGTVVRGQIESLSTAARFRA
                     FNAENRLVADKAWKEGSSLSHGDAIDFLLSWGREDANRGYSIVGAGHRVLHGGLKYDR
                     PVRIDRAVLDSLQTIAPLAPLHQPHNLAAINAVARLAPGLPQVACFDTAFHRTQPALA
                     QMFAIPRSYTERGIRRYGFHGLSYEFIASELPRLDPKAAAGRTIVAHLGNGASMCAMV
                     AGRSVATTMGLSALDGLVMSTRCGSIDPGVLIYLAAHEHLDMPRIQNLLHAESGLLGV
                     SGLSGDMRTLLASSDPRAKEAIDLFVYRAAREIGSLAAAMNGLDALVFTGGIGEHAAP
                     VRAAICEASRWLGVELDTPANVRHGTRISASTSRVSAWAIPTNEELMIARHTLATLTT
                     HS"
BASE COUNT         1653 a         3355 c         3208 g         1763 t
ORIGIN      
        1 tcaacgtgtc caccaagctc gcggggcgca tcgaagagat gctcgtggat gagggcgact
       61 tcgtcgaggc cgggcaggcg ctggtcagga tgcagaccga cgtgctcgtg gcccagcgcg
      121 ccgaggcggt cgcgcagctc cagcaggcgc ggcaggccgt ggccaccgcc accgcgcaga
      181 tggcgatgcg cgaggccgac ctcgcggcct cgcaagccct gatcgcgcag cgggagagcg
      241 agctgagcta ttcgcggcgc cgactgacgc ggtacgagtt ggccggcgag aagagcgcca
      301 cttcggttca agagatcgac gaagagcgca cgcgctcgct cgtggcggaa gcgaccttga
      361 cggccgccaa ggcgcaggcg acggccgcgc aggcggcgat cgaggcggcg cgcgcggaga
      421 tcgccggggc ggaatccacc gttcatgcca gggaagcgac ggtgcagcgc atcgaggcgg
      481 acatcaagga cagcgtgctc gcgtctccac gcgccggccg cgtgcaatac cgcatcgcgc
      541 agccgggcga ggtactcgcc gcgggggggc gggcgctcaa cgtgatcgac ctggccgacg
      601 tgtacatgac gttcttcctg cccgaacagg tggccgggag agtggcgatg ggaacggagg
      661 tgcggctggt gctcgacgca gcgccagggt ttgtcattcc cgcggaggtc tcgttcatcg
      721 cgagcaccgc ccagttcacg cccaagacgg ttgagacggc cgaggaacga cagaagttga
      781 tgtttcgggt gcgggcgcga atctcgcccg atctgctccg gaagcacctt catcaggtga
      841 agaccggtgt cccgggggtc gcgtggatca aactggatcc cgccgcccat tggcccgctg
      901 atcttaccgt gaaggtgccg gagtagcgtg tgaacgatgc cagcccgtcc agttcggttg
      961 cccgccttca tgacgttgcc ttgcgctacg ggcgaacgat cgcgctgtcc ggcgtgagcc
     1021 tcgacatccc ggcccgcacg atggtgggtc tcatcggtcc agacggggtt ggcaagtcga
     1081 ccctgctttc tctcatcgcc ggcgcccgcc gggtgcagca ggggatggtg gaggtgctgg
     1141 gaggggatat ctgccgttcg tcgcaccgcc gcgatgtctg cacacgcatc gcctacatgc
     1201 cccagggctt gggaaagaac ctctacccca ccctctcgat cgaagagaac ctgcagttct
     1261 tcgcccgact gttcggtcaa gactcggcgg agcgccgccg gcgcatcgat gacctgacca
     1321 ggagcaccgg gctgtttcga ttcctcgatc ggcccgccgg caagctctcc ggcggcatga
     1381 aacagaagct tgggctctgt tgctcgctca tccacgaccc cgacctgctc atcctcgacg
     1441 agccgacaac gggcgtcgat ccgctggcgc gccggcagtt ctgggatctg atcgcgcacc
     1501 tgcgccagga ccgcccggag atgagcgtgg tcgtcgccac cgcgtacatg gaggaggccg
     1561 agcgattcga ctggctcgtg tccatggacg aggggaaagt cctcgcgacc ggcacgccga
     1621 ccgagcttca cgagcggacg gggacggact cgctggaggc ggcgttcatc gagctattac
     1681 cggaagagaa gcggcggggc cacaagcccg tgcagattgc gccgtttgtc ggcggcgagg
     1741 ccgacgagat cgccatcgaa tcacgcggtc ttaccaagcg gttcggtaac ttcgtcgcgg
     1801 tcgatcatgt caccatgcgg gtgcggcgcg gcgagatttt cggcttcctg ggatccaacg
     1861 gctgcggcaa gacgaccacg atgaagatgc tcaccgggct tctgcccgcg agcgagggcg
     1921 aggcggaact gttcggccgg ccgctcgatg ccggcgatct ggccacgcgg gggcgtgtcg
     1981 gctacatgtc gcagtcgttc tcgctctaca aggagctcac cgtccggcag aacctcgtgt
     2041 tgcacgcgag aatcttcagt ctgccggccg gcgagatccc cggccgggtg gcggagatgg
     2101 gtgaacgctt cgggctgacc cgcgtcatgg atgcgctccc ggacaacctg ccgctgggga
     2161 tgcgccagcg gctgtcgctg gcggtggcca tggtgcatgg gccggaactg ctcatcctcg
     2221 acgagcctac ttcaggggtc gatccaatcg cccgcgactc actttgggcg ctgatgatcg
     2281 agttgtcgcg tcgcgatcgg gtgaccatct tcatctcgac acacttcatg aacgaggcgg
     2341 agcgctgcga ccgcatctcg ttcatgcacg ccggcaaggt cctcgtcagc gatgcccccg
     2401 cggacatcgt tcgcgaacgc ggcgtggcga gcctggaggc cgcgttcatc gcgtacctcg
     2461 ttgatgccgg cgcggagacg acgcacgccg gcgcggcagc cgccgcgaca ccacgcacag
     2521 cccctgcgac ggatgagcta cctaccagcc gaaccgccgt ccatcgttcc gatcggagca
     2581 tgttcagcgt gcaacgcgca ctgagctact cgctgcgcga ggctctcgaa cttatccgag
     2641 accccgtccg tggcgcactt gccctccttg gcagcatcgt cttgctgctt gtcatcgggt
     2701 acggcatcac gatggatgtt gaaaacctct cctacgccgt gctcgaccgg gaccagacgg
     2761 tgctcagccg cgactacacg atcaacctct ccggctcccg gtacttcacg cagcgcccgc
     2821 cccttgccga ctacgccgag ctcgaccacc ggatgcgcac gggcgaactc tccctggcga
     2881 tcgagattcc gcccgggttc gggcgcgaca tcgagcgtgg cgaccccgtc gagatcggcg
     2941 cgtgggccga cggcgcgatg ccgcagcgtg ccgagaccgt ccgcgggtac gttcagggga
     3001 tgcaccaact ctggctgcag gaacagtacc ggagacgcgg gatcgacgtc gccgccaacg
     3061 ccacagtcga gtccaggtac cgctacaacc ccgacgtgcg aagcctgccg gcgatggtcc
     3121 ccgccatcgt gccgctcatg ctcctgatga tccctgcaat gctcgccgca ctttccgtgg
     3181 tgcgggagaa ggaactcgga tcgatcatca acttctacgc cacgccggtc acccgcgccg
     3241 agttcctgct cggcaagcaa ctgccctata tcgccctcgg tgtggtgaac ttcctgctca
     3301 tggccgcgct cgctgtgacg attttcggcg tgccgatgaa ggggagtttc tgggtgctgc
     3361 tgctggcgtc ggggctcttt gtaggcttcg cgacggggtt cgggctgttc gtgtccacgc
     3421 tcgtgcgcgg ccagatcgcc gcgatcttcg cgacgatgat cgccacgatg atcccgacca
     3481 tccaatacgc ggggctcatc aacccggtcg catcgctgga agggatcggc gcggcgatcg
     3541 gccgggtcta ccccgcgacc cacatgatca tcgtcagccg cggtgtgttc aacaagggcc
     3601 tccgcctgca cgacctgcac gacgcgttgt gggcgatcgg tctatccgtt cctgtcatcc
     3661 taatcgcggc cgtggcgatg ctccggaagc aggagcgatg atgaaacagc acccgctcgt
     3721 catcattgcc cacttggggg tcaaggagct ccgcgggctc ttgcgcgacc cggcgatgct
     3781 cttcctcatc gtctttgctt tcaccgctgc catctacacg gccggcacgg cgatgcccga
     3841 gagcatgcac aatgcgtcca tcgccatcgt ggatgaagac cggtccgccc tgtcgtcgcg
     3901 catcattgcg gcgctccagc caccgtactt cacgccaccg cgccatgtct ggctggacga
     3961 ggccgacgcg gggctcgacg ccgggcgttt cacgtttgtg ctgaacatcc cgcccaactt
     4021 ccaacgcgac gtgctggcgg gcaaggcgcc agcggtccaa ctcaacgtcg acgcgacgcg
     4081 gatgagccag gcgttctccg gcaacgggta tgtccagcag atcgtgcagg ccgaggtgga
     4141 cgagttcgtc tccggcgtcc gcgccaggcc tcggacgccc gtcgaactgg ccctgcggat
     4201 gcggttcaac cccaacctcc agcagtcgtg gttcggttcg ctcatggaga tcatcaacat
     4261 ggtcaccatg ctgtcgctca tcttgaccgg ggccgccctc atccgtgagc gagagcacgg
     4321 caccatcgag cacttgctgg tgatgcccgt cacgcccgcc cagatcatga cggccaaggt
     4381 ctggtcgatg ggcttggtgg tgctcgtcgg cgccgcgttc tcgctgacgg tcatggtcca
     4441 gcgtgtgctg catgtcacgg tgcacggctc ggtcgcgctg tggctcctgg ccgcggcgct
     4501 acacctggtc gcgacgacct cgatgggcat cttcatggcg accgtggcgc ggtcgatgcc
     4561 gcagttcgga ctgcttgtcg tgctgacgat catccccttg cagttgctct ccggcggcac
     4621 gaccccgcgg gagagcatgc ccgaggcgat ccgcacgctc atgctcgcgg cccccacgac
     4681 ccatttcgtc tccgcaagcc aggccatcct ctaccgcggc gcgggcttgg aaatcgtctg
     4741 gccccagctc gcgaccgtgc tcgggatcgg aacggtcttc ttcaccatcg ctctccgccg
     4801 cttccgcggc acgctgaacc ggatggcgtg agccgcgtgt tgctgtggca tgctcctcca
     4861 cgtccgattc accgcttcag acccgtgctg ggacaacagc ggctggggaa tgggaatgcc
     4921 gatcatcgag aacaacttcg aagggagcga cttctgatgt cagcatccgt cattgcaacg
     4981 tccgttctcc tcgaatccac cgacttgcgc cgcggctgct ccggctcgag cgacatggtg
     5041 actcccaccg tcgtgtcggc tccgcgatta cgagtgtggc tnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn ntgacaagga ggtgctgcac gccatcgccg tcgagcacca
     5221 caaaggccgc cggctgctca tcgcaacgac gaacctcgac gccaaccgcc ccgtcatctg
     5281 ggatcttggc gcggtcgctg ccagcggctc tccccaggct ctcgacctgt tccgagatat
     5341 catcatggcg tcggccgcgg tgccggtgct cttcccgccc gtgtacattg acgtggagtg
     5401 tgatggcatc gtctacgacg aaatgcacgt tgatgggggt gtgacgtccc aggttctttt
     5461 gtatggaagc gccgtgtcga ggcacgatgt cgtgcgtgag tcctcgccaa acgcgccgag
     5521 cgccacctat tacgttatcc gcaacggcaa gttcgcaacc gactgggcac ccgtgaggcc
     5581 aagggtgctg gatattgcat ctcgatctat cagccggatc atccaagccc agggagttgg
     5641 ggacttgtgg caggtgtacg cggcatgtcg cagggatggc cttgaattca ggctcgcatt
     5701 cattcccgat gaaatcgctc tccctaccga tcctgagttc gataccggtg tcatggcgga
     5761 cctgtttgaa cgtggccgaa cgctcgcacg cgagggttat ccgtggcgca ccgaaccccc
     5821 cggacttcgg agcaatggac cgtatgatga ggcgagaccg ggaccatctg gttcaggccg
     5881 cggaccgacg aatcccctgt gcagagccgt ttatcaacac gccgggcgcc catgatgaat
     5941 ccgtctacgc acgaacccgt ggcgaagcct gacccggccc tcgtggtcgc cgccttcacg
     6001 cagacgtcgg agaacatccg ccacatccga agcgagcgca tctggtttgc caacgcctac
     6061 ggcgccatcg tcgcgggcgg gctggccctc ctcccccgcg atctgagcat cgtatccaat
     6121 cgccgcgtcg tgctcgcgag cctggttgtg ctcgtgctgt tctccggggt cgcgctggcg
     6181 tgcagcatcc gactggtggc ggagcttcgc accgccctgg cgaacctagc ccgaatcgcc
     6241 tccgacaacg ggatggaccg gcttgtcggt gacatcgaca cgccccggtc attcggcgcc
     6301 cgcatgccga tgcgctgggt gttcccgatc tacttctcga tcaccacggt ctgccttctg
     6361 gtgctgctga tcagtcacct gttggcctga gccgctcccc ggatggcatg agcaatggcc
     6421 agagaggcga tgcgccgcag gagtcccggt tggtcgaatc catcaagtct ccacacgccc
     6481 agccgtcgcc cgccctcgcg gtaacgcggc gctggagcgt ggtggtcgtt ctggtatccg
     6541 tcatggccgc gttgctggtc tacgccgcgc tcccgcggga tatgggcgaa ctcgggcgtc
     6601 gggcggtcgg gatcgcggtc ttcgcggcgg cgctgtgggc gacggaggcg atcccgctct
     6661 tccccgcctg gctcagcatc gtcgggctcg atgtgctgct gctctcgcgc gggggggagc
     6721 ggcgggctgg cgggcacggg caacctcagc tacacccagt tcttcgagcc gttcgcgtcg
     6781 agcacggtca tcctgttcca aagtgggttc ctgttgaccg cggcggtgca gaagcacggg
     6841 ctggaccgcg cgatcgcctc gcagttggcg cggccgttcg tcggcgactg taccgactcg
     6901 tcatggccgt catcctgacg acggggttcc tgtcgatctg gatcagcaac acggcgacgg
     6961 cggcgatgat gctggcgatc gtgacgccgc tggcgcggaa tccggccacg ccgcggcggc
     7021 tcgggtcgcg ccacgccgcg gttactttgt ttgccgccgc tgatgccatt cgttgcctcg
     7081 cctcgatacc atcagcatct gacgtgcatc atcgccgcga cgccaagccg ccgcgtcgct
     7141 tggagtctgc ccatgacgcg cccaatgaca ccaaagtcca ctcatggtca taccattcgt
     7201 agatcggctt cgggaggagc acagtccggc ccgctcagtt cgcgcgacct tgaactgatc
     7261 gacgcttatt ggcgcgcctc gaactacctc tccgtcgggc agatctacct gctggacaac
     7321 ccgctgctgc gccagccgct gaaacgcgag cacgtgaagc cgcggctgct cgggcactgg
     7381 ggaacaacgc ctgggttgaa cttcatctac gttcacctga accgcgtcat caagcgcgac
     7441 gacctgagcg tgctctacat cgccggcccg ggacacggag gccccgcgct ggtggccaat
     7501 gtctacctcg aggggacata ctcggaggtc taccccaaca tttcgcagga cgaggagggg
     7561 atgaagcgat tgttcgtcca gttctccttc ccgggcggca tccccagcca cgtcgcgccc
     7621 gagacgccgg gatcgattca cgagggcggc gagctcggct acgcgctctc gcacgctttc
     7681 ggcgcggcgt tcgacaaccc cgatctcatc gtcgcctgcg tcgtcggcga cggtgaagcg
     7741 gagacgggcg cgctggcgac gagttggcac tccaacaagt tcctcaatcc cgtgaccgac
     7801 ggcgcggtgc tgcccatcct gcacctgaac gggtacaaga tcgccaaccc ctgcgtgctc
     7861 gcccgcatcc cccacgaaga actcgatgcg ctcttccgcg gctacggcta cgcgccgatc
     7921 tatgttgaag ggcacgagcc ggagaagatg caccagttga tggccgcggc gatggacaag
     7981 gcgttcgccg acatccgcgc catccagaac gacacccggg ccaagggctt caagcagcgg
     8041 ccgacctggc ccatgattgt gcttcgatcg cccaaaggct ggacctgtcc ggcggagatc
     8101 gacggcaaga agtgcgagga ctactggcgg tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnccgcagtg gctcaccatg gaccaggcgg tgaagcactg cgaggcgggc
     8281 ctggggatct gggagtgggc gagcaatgac atgggtcacg agccggatgt cgtgatggcc
     8341 tgctgcggcg acgtgcccac gctggagacg ctcgccgccg tcgacctgct ccgcgagcac
     8401 tttccggaga tcaggatccg cgtcgtgaac gtcgtcgacc tgatgacgct ccagtcgccg
     8461 agcgaccacc cgcacggact gagcgaccac gacttcgaca cgctcttcac caaggacaag
     8521 ccgatcgtgt tcgccttcca cggctacccg tggctgatcc accggctgac ctatcggcgc
     8581 acgaaccatg agaacctgca cgtccgcggc tacaaggaag agggcaccac gacgacgccg
     8641 tttgacatgt gcgtgttgaa cgaactggat cgcttccacc tggtcggcga cgtgatcgat
     8701 cgcgtgcccg cattgggcgc gcgggccgcg tatgtgaaac agcggattcg gaatgccttg
     8761 atcgatcacc gcgagtacat cacccggcgc ggcgaagacg accctcgcat cgtcggttgg
     8821 aagtggggcg gcggcaagac cgcttcgacg cgcagtgcga ccgaagggga caatgtgtag
     8881 accaccatga acgacgccct cctcatcctg aacgccggct catcgagcgt gaagttctcc
     8941 gttttcgatt cgcccgggct ggggaccgtc gtccgtggcc agatcgaatc gctctcgacc
     9001 gcggcgaggt ttcgggcttt caacgctgag aacaggcttg tggcggacaa agcgtggaag
     9061 gaagggtcat cactctcgca cggcgacgcg atcgacttcc tgctgtcgtg gggccgggag
     9121 gacgcgaatc gcggttacag catcgtgggt gcgggacacc gggtgttgca tgggggtctg
     9181 aagtacgacc ggcccgtgcg gatcgaccgc gcggtactcg acagtttgca gacgatcgcg
     9241 ccactcgccc ccctccacca gccgcacaat ctcgcggcca tcaacgctgt cgcgcgcctc
     9301 gcacccggcc tgccgcaggt ggcatgcttc gacaccgctt ttcaccgcac ccagcccgcg
     9361 ctcgcgcaga tgttcgccat cccgcgctcg tacacggaaa ggggcatccg ccgttacggg
     9421 tttcacggtc tgtcctacga gttcatcgcg agcgaactac cgcgactcga tccgaaggcc
     9481 gcggccggac gaacgatcgt cgcccacctc ggcaacggcg cgagcatgtg cgcgatggtc
     9541 gcaggtagga gcgtggcgac gaccatgggc ctgagcgcct tggatggtct ggtgatgagc
     9601 acccgttgcg gttcgatcga ccccggcgtg ctcatctacc ttgcggccca cgagcacctc
     9661 gacatgccgc ggatccagaa cctcctgcac gccgaatccg gcctgctggg cgtctcgggc
     9721 ctctccggcg acatgcgaac tctgctcgcg tccagcgacc cacgggcgaa ggaagccatc
     9781 gacctgttcg tgtaccgcgc cgcccgcgag atcggctccc tcgccgcggc catgaacggg
     9841 ctggatgcgc tggtcttcac cggtgggatc ggcgagcacg ccgcgcccgt ccgcgcggcc
     9901 atctgcgaag cctcccgctg gctcggcgtg gaactcgaca cgcccgccaa cgttcgccat
     9961 ggcacgcgca tctccgcgtc gacctcccgc gtctcggcct gggccatccc caccaacgaa
    10021 gaactcatga tcgcccgtca cacgctcgcg accctcacca cgcactcctg aaccagcatc
    10081 catagtagag cctatcccga aaccgttctg ccggggcgcg tatgagtggg caccatgctg
    10141 acactcacgg acgagcagtt ggactggctg gccgagcgg
//
LOCUS       DAOQNN010000072        10151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3110_length_10151_cov_10.678982, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000072 DAOQNN010000000
VERSION     DAOQNN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3110_length_10151_cov_10.678982"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3528)
                     /locus_tag="PKV97_06010"
     CDS             complement(<1..3528)
                     /locus_tag="PKV97_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05470.1"
                     /translation="MNAEVRIEQRCPYVGLQPFEEADRAFFFGRERDQRVIIANLLSS
                     PLTILYGSSGVGKSSVLMAGVVPQLHRERPRTPVVVFRNWVDRDFQLALTRACIETVW
                     AMQVDQPRPAETLPLDEVLRACAEAAHGTILVIFDQFEEYFLYHAKDSEAAGAFEAQF
                     ARAVNREDVDVGFLVALRDDSLSRLDRFQERIHNLLSNRLRLNHLEPEAAGDAIRKPL
                     EVWNALQAVGVPPVGIDDELVDELLRQVRIGQVSAGRPGGSGGLRHDEGCIEAPFLQL
                     VMVRLWEEERAQGSDRLRRETLDRLKGADEIVRTHLDVAMARLDATSQAVCASFFDRL
                     VTPSGSKIACGAADLISWAGDLAPHVPAVLQSLSDARNRILRTVSVPDNPEVMRYEIY
                     HDVLAPAILDWRQRFEADRQREEALRVAREKAARREEEAQIARERAARRALHRWLVGL
                     GMMSCVAGAGWLYASWEGMRSAANEKAAQSISNAPIDPGSALSLALEAVEATVPSWMS
                     VARAARLHLTPTAAAEDALRQAIQASRLVWTLPVSGRALSDVAFSPDGGRLLTAGQDQ
                     TARVWDIRSGRPAEPVVTFAHDAWVRRGRFLPDGERVITVSGGLAYLWSLAQPTAPLR
                     SFEHGGNIYSAFDTSADGRRLATAGDGAQKARVIRIWDVDADVPEPLLTLDAEGAWIM
                     GLAFSPDGCCVATAAVERGKAERSAIALWGLERRREILSLPLEEPSDAIAFAPDGSAL
                     VTASRDGWLRVWEPAGKGFEVLLREAAGGTHRGEVLAWNGRTLAGHGDRIRNVEVSAD
                     GALIASAGGDGTARVWSRHTGENLLTLTGHLSYVEAARFSPDGRHVATASRDGTVKFW
                     NIEGHANTVTSIAFSPDGRTLATGSSDRTARIWDFADGTPRLRHTLRGHADQVHRVAF
                     DPSGARLATAGLDNQLRLWDVASGEAVTLPSVPPEHRHKDQLRGVAFSPDGRLLASAG
                     ADGHARLYPLAGEGSPVSVLHGGATIQVSGVAFHPGGERWATSGFDGKLKLWDLSGTD
                     LGTVGLPDDERKLGIRIAGLAFSPDGEYVAAVVQRRVRVWPVAAFKEPEVHPPVRLPL
                     IEGARHCAALAYSPDGRWLAVGCSDAGVRLFEMPAGTLVKTITVHKNEVRGVAFSPDG
                     TLLATASNDKTFHVSPLAFEAVYEAARRLQ"
     gene            complement(3525..5069)
                     /locus_tag="PKV97_06015"
     CDS             complement(3525..5069)
                     /locus_tag="PKV97_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HNB05471.1"
                     /translation="MRLRFFLSSPGDVADERTFAQQVIEQELPKDPFMRGRVGCEAVR
                     WDDPAAPVAMPATLTPQEAVNRGLPRPSACDCVIVVLWSRLGTPLPASCTRPDGSRYL
                     SGTEWEYEDAIAARPQPHILVYRRRSPPELGDPLDADYDERLAQYRRANDFFKRFRNA
                     DGSLAGGFAEYDTPQEFRDRLRVDLRAFIQRRLDSEQANGQTVRVNAPPPYGRIAKAL
                     ASGSAVPIIGPGICRSGRPGDEPWDPDAPRFLPSGNELSACLADEAGFPSQAERDQLA
                     EVASYYEAFKTRDALRERLRSLLAPAAPDKLAVPPLYSLLASLEKPLLIITTNYDTLI
                     EQVFRNAQKPYDLVVYSELKELGNAVLWWPHGALKPETVAPNELSIELEKKTVIFKMH
                     GSIVPQTSDLDTFVITESDYVELLARIASRSAIPALFASHCQDRNLLFLGYSLRDWSF
                     RTILQSLNRFFAKRLVAGDDEIQSWAIDDRFSELELKFWQRRGVYPYEVNLDEFVRLL
                     RARMSP"
     gene            5317..7116
                     /locus_tag="PKV97_06020"
     CDS             5317..7116
                     /locus_tag="PKV97_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNB05472.1"
                     /translation="MPLPELHAPLFVGLVLVVLFVAFVREWVKPDVAVMVAVGVLLVA
                     GMLNTKEVLGVFGNSAPITIACLFIISAALSRTGCVDQLGEWLAAAAGAGERRLLLAL
                     IAACLLVSPFINNTPVVMVMIPAVIAVAARQGLAPSRLLIPLSYATILGGLITMVGTS
                     TNILVDGVAREQGLAPFSMFEISLPAILMALVGSAFMLVFAPRLLPVRETLSQQFGAG
                     ERWFMTELFVPEGSHLAGRTLREARLSNGIIQVLNLVRGDTELADPAPDTRLEVGDRV
                     VVHSRSDAMMALRSSDGVGLQAPAGAEAHDIETLRRRDLVMVEAIVGQTSRYILRPIR
                     DLDLLARYGIHLVAVHRRDASFAQIGEDFQLLGGDVLLVEGTPAQIQRFCDNGDLFAI
                     TEGRQMASRRHKAPVALATIVGVMLLAALNVMPIEGLALIGAAIVIATGCITSDEAYK
                     SIEWPILVLIFGMLAISIAMRNSGLDQLLAAQLAALGEGMSPWLMLSLVILITSVATE
                     VLSNNAIAVLFTPVVIGLAQQMGVDPRPFVVGVMFAASCSFATPIGYQTNTLVYSAGN
                     YRFADFARLGVPMNILTWLLCSALIPLFWPFQP"
     gene            complement(7138..>10151)
                     /locus_tag="PKV97_06025"
     CDS             complement(7138..>10151)
                     /locus_tag="PKV97_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNB05473.1"
                     /translation="RLLAAAIAVAVLVAVVAGGLLLWHEAGTSRLQARELARYAARLD
                     YALLAGPSAAIRFPAHGPFDQRLGYTELARFSERLQARGFALVEQVRFDDALLAHVER
                     GLFPPYAEKTRAGLDVFDCRGEPLYGFRYPWRGYERFEDVPAIVAQALLFIENRELLD
                     ESRPTLNPAVDWVRFARAALGQLGRMVDADLDAPGGSTLATQIEKYRHSPGGITQDAR
                     EKLRQMVSASVRAYREGEQTLPLRRRLVLDYLNTVPLSAAPGHGEVNGLGDGLWVWFG
                     AEFARVNALLAAPVGEGEERIAQGRALRQVVALMIAHRRPSWYLAGGREELARTTDAY
                     LRLFAEAGLIGAVLRDAALARPLAFRDLDADPAWVPATPGKGTTAVRTRLAGLLDTSL
                     YSLDRLDAELGTTLHGGLQQAVGDYLGQLSDPAFARSQGLIGERMLQPATLGAVRYSF
                     TLVERTAGGNRVRVQTDTTDQPLDINEGSKLELGSTAKLRVLATYLELVAELHGRLAA
                     LDPPALRRVEVDAQDRLTRWAIDHLASAPDRSLPAMLEAALERRFSASPGEGFFTGGG
                     RHTFGNFNASDNARNPTLREALQASINLPFVRLMRELVRHTMYQVPGSTARLLEDNED
                     PRRVEYLVRFADREGQLFLRRFWRKYQDSTAEEIRSTLLDGLRPGADRLAAVFRYLEP
                     EAPPHALADFLARRLGEPPAAARLAQLYARNAPEAFDLPDRGYVARVHPLELWLVAFR
                     LQHPEATLAEAVAASADERQQVYRWLFRTRAKGAQDSRIFTLLEVEAFLEIHRRWARL
                     GYPFGHLVPSLATALGSSGDRPAALAELMGIIVNDGQRLPVQRIDTVRFARATPYESA
                     FAIRPVEGDPVMAPEVARALRGALSDVVEGGTARRLAGAFKLADGSELALGGKTGTGD
                     NRIVVRGRAGLALNRTATFVFYLGPRHFGTLTAYVIGPEAASYRFTSGLPVQILKSMG
                     PLLIPHLEPAGAHACLPPPLSEAAPQPVTAG"
BASE COUNT         1505 a         3597 c         3593 g         1456 t
ORIGIN      
        1 ctgcaggcgc cgcgcggcct cgtacaccgc ctcgaaggcg agcggggaga catggaaggt
       61 cttgtcgttg ctggccgtgg ccagcagcgt cccgtccggg ctgaaggcga cgccgcggac
      121 ctcgttcttg tgcaccgtga tggtcttgac gagcgttccc gcgggcatct cgaacaggcg
      181 cacgccggcg tcgctgcagc ccaccgccag ccagcggccg tccgggctgt aggcgagcgc
      241 cgcgcagtgg cgcgcgccct cgatgagcgg caagcgcacg ggcggatgga cctccggctc
      301 cttgaaggca gcgaccggcc agacccgcac acgtcgctgc accaccgcag cgacgtattc
      361 tccatccgga ctgaaggcga ggccggcgat gcggataccg agcttgcgct cgtcatccgg
      421 caagccgacc gtgccgagat cggtcccgga cagatcccac agcttcagct tgccgtcgaa
      481 gccgctggtc gcccagcgct cgcccccggg atgaaaggcc acgcccgaca cctggatcgt
      541 cgccccgcca tgcagcacgg agaccgggct tccctccccc gcaagcggat agagccgtgc
      601 atggccgtcg gccccggcac tcgccagcag gcggccgtcc ggactgaagg ccacccccct
      661 gagctgatcc ttgtgccgat gctcgggagg cacgctcggc agcgtgaccg cctcgccgct
      721 cgcgacgtcc cagaggcgca gctggttgtc cagcccggcg gtcgcgagcc gcgccccgga
      781 agggtcgaag gccacgcgat gcacctgatc ggcatgaccg cgcagggtat ggcgcagtcg
      841 cggggtaccg tccgcgaagt cccagatgcg ggcggtgcgg tcgctgctgc cggtggccag
      901 ggtacgacca tccgggctga aggcgatgct ggtgaccgtg ttggcgtgcc cctcgatgtt
      961 ccagaacttg accgtgccgt cacggctcgc cgtcgccacg tgacgcccgt cggggctgaa
     1021 gcgggccgcc tccacgtagg agaggtgccc cgtcagcgta agcaggttct cgcccgtatg
     1081 ccggctccag acccttgccg tcccgtcgcc gcccgcgctg gcgatcaggg ccccgtccgc
     1141 actcacctcc acattgcgga tgcggtcgcc gtgaccggcc agcgtccgcc cattccacgc
     1201 gagcacctca ccccggtgcg tgccgcccgc cgcttcgcgc agcagcacct cgaacccttt
     1261 ccccgcgggc tcccacaccc gcagccagcc atcacggctg gcggtgacca gcgcgctgcc
     1321 atcgggcgcg aacgcgatcg cgtcactcgg ctcctcgagc ggcagggaga ggatctcgcg
     1381 ccggcgctcg agcccccaca gggcgatcgc gcttcgctcg gccttgccgc gctcgaccgc
     1441 agcggtggcg acgcagcagc catccgggct gaacgccagc cccatgatcc atgccccctc
     1501 cgcatccagg gtgagcagcg gctccggcac gtccgcgtcg acgtcccaga tgcggatcac
     1561 gcgcgccttc tgcgcgccat ccccggcggt cgcgaggcgc cggccgtcgg cgctggtatc
     1621 gaaggcagag tagatgttcc cgccatgctc gaaactgcgc agcggagccg tcggctgcgc
     1681 gagggaccac aggtaggcca ggcccccgga caccgtgatg acgcgctcgc catcgggcag
     1741 gaagcggccc cgccgcaccc aggcgtcgtg cgcgaaggtg acgacgggct cggcgggacg
     1801 gccgctgcgg atatcccaca cccgcgcggt ctgatcctgg ccggcagtga gcagccggcc
     1861 gccatcggga ctgaaagcca catcggagag cgccctgccg ctcaccggca gggtccacac
     1921 caggcgcgag gcctggatcg cctggcgcaa ggcgtcttcg gcggcagcgg tcggggtcag
     1981 gtgcaggcgg gccgcgcgag cgaccgacat ccaggacggg accgtcgcct ccaccgcctc
     2041 cagcgccagg ctcagcgcac ttccgggatc gataggcgcg ttcgagatcg actgcgccgc
     2101 cttctcgttg gccgccgaac gcattccctc ccaggacgca tagagccatc ccgcaccggc
     2161 gacgcaactc atcatgccca ggcccacgag ccacctgtgc aaggcgcgcc gggccgcgcg
     2221 ttcgcgcgcg atctgcgcct cctcctcgcg ccgtgcggcc ttctcgcgag ccacccggag
     2281 cgcttcctcg cgctgccgat cggcctcgaa gcgctggcgc cagtcgagga tggcgggcgc
     2341 gaggacgtcg tggtagatct cgtagcgcat cacctccggg ttgtcgggga cggacacggt
     2401 gcgcaggatg cggttgcgcg cgtcgctcaa ggactgcagc accgccggga cgtggggcgc
     2461 gagatccccc gcccagctga tcaggtccgc ggcgccgcag gcgatcttgc tgccgctcgg
     2521 ggtgaccagc cgatcgaaga agctggcgca cactgcctgg ctcgtcgcgt cgaggcgggc
     2581 catcgcgacg tcgaggtggg tgcgaacgat ctcgtcggca cccttcagcc ggtcgagcgt
     2641 ctcccgccgc aaccggtccg agccttgcgc gcgctcctcc tcccacagtc gcaccatgac
     2701 cagctgcagg aagggcgcct cgatgcagcc ctcgtcgtgg cgcagccccc cgctgccccc
     2761 ggggcgtccg gccgagacct ggccgatgcg gacctggcgg agcagctcgt cgaccagttc
     2821 atcgtcgatg ccgaccggcg gcacgcccac cgcctgcagc gcgttccaca cctccaacgg
     2881 cttgcggatc gcgtcgccgg ccgcctccgg ctccagatgg ttgagccgca ggcggttgct
     2941 cagcaggttg tggatgcgct cctggaagcg gtccagccgc gacaggctgt cgtcgcgcag
     3001 cgcgaccagg aaaccgacat ccacgtcctc gcggttgacc gcgcgcgcga actgggcctc
     3061 gaacgcgccg gccgcctcgc tgtccttggc gtgatagagg aagtattcct cgaactggtc
     3121 gaagatcacc aggatcgtgc catgcgcagc ttcagcgcag gcgcgcagca cctcgtcgag
     3181 gggcagggtc tccgccggcc tgggctggtc cacctgcatg gcccagacgg tttcgatgca
     3241 ggcgcgcgtc agcgcgagct ggaagtcgcg atccacccag ttgcggaaca ccaccaccgg
     3301 ggtccgcggc cgctcgcgat gcagctgcgg cacgacgccg gccatcagga ccgagctctt
     3361 gcccaccccg ctcgatccat aaaggatcgt cagcggggac gagagcaggt tggcgatgat
     3421 cacccgctgg tcgcgctcgc gcccgaagaa gaaggcccgg tcggcctcct cgaagggctg
     3481 caggccgacg taggggcaac gctgctcgat gcggacctcg gcgttcatgg cgacatcctc
     3541 gcgcgcagca aacggacgaa ctcgtccagg ttcacctcgt agggatacac cccgcgccgc
     3601 tgccagaact tgagctcgag ctcggagaag cgatcgtcga tcgcccacga ctggatctcg
     3661 tcgtcgccgg cgacgaggcg cttggcgaag aagcggttga ggctctgcag gatcgtgcgg
     3721 aagctccagt cgcgcagact gtagccgagg aacagcagat tgcggtcctg acagtgcgac
     3781 gcgaacaggg ccggaatcgc cgacctgctg gcgatgcgcg caagcagttc gacatagtcc
     3841 gactcggtga tcacgaaggt gtcgaggtcg gacgtctggg gaacgatgga gccgtgcatc
     3901 ttgaagatca ccgtcttctt ctcgagctcg atggaaagtt cgttcggcgc cactgtctcc
     3961 ggcttcaggg cgccgtgcgg ccaccacagg acggcgttgc cgagctcctt gagttcggag
     4021 tacacgacca ggtcgtaggg tttctgcgcg ttgcggaaga cctgctcgat cagggtgtcg
     4081 tagttggtcg tgatgatcag cagcggcttc tcgagactgg cgagcaacga gtagagcggc
     4141 ggcacggcga gcttgtccgg cgcggccggc gcgagcagcg aacgcagtcg ctcgcgcagg
     4201 gcatcgcggg tcttgaaggc ctcgtagtag gacgccacct ccgccagctg gtcgcgttcg
     4261 gcctgcgacg ggaaccccgc ctcgtcggcc aggcacgccg acagctcgtt gccgctgggg
     4321 agaaagcgtg gtgcatcggg gtcccagggc tcgtcgccgg ggcgccccga gcggcagatg
     4381 ccgggaccga tgatcggcac cgcagaaccg ctggcgagcg ccttcgcaat ccgtccatac
     4441 ggcggcggcg cgttgacgcg tacggtctgc ccgttggcct gctcgctgtc gagtcggcgc
     4501 tggatgaagg cgcgcagatc cacgcgcagg cggtcgcgga actcctgcgg cgtgtcgtac
     4561 tcggcgaagc cgccggcaag cgaaccatcc gcgttgcgga agcgcttgaa gaagtcgttc
     4621 gccctgcggt actgggcgag gcgctcgtcg tagtccgcat cgagcgggtc gccgagctcg
     4681 ggcgggctgc gccggcgata cacgaggatg tggggctgcg gcctcgcggc gatggcatcc
     4741 tcgtactccc attcggtgcc cgagagatag cggctgccgt cggggcgtgt gcagctcgcc
     4801 ggcagcggcg tccccagccg cgaccacaac acgacgatca cgcagtcgca cgccgaaggc
     4861 cgcggcagcc cgcgattgac cgcttcctgc ggcgtcagtg tcgccggcat cgccaccggt
     4921 gccgcagggt cgtcccagcg caccgcctcg caaccgaccc ggccgcgcat gaagggatcc
     4981 ttcggcagct cctgctcgat gacctgctgg gcgaatgtgc gctcgtcggc aacatcgccc
     5041 ggggacgaca ggaagaaacg gagacgcatg actttcccct cctaggcggc ttcgcgggac
     5101 ccgcatgaca aatataggcc gacaacggcg agatcgaacc ccctgcgcca ctgcaggcac
     5161 ccgctgccgg gctgcggcgc cgaggtggat cgccgcgcac cgcccgactt cagccaccgc
     5221 tcgaatgaac cgagaggaat aagctatgga atatcaagtc ttttgaatgc ataaacgcgg
     5281 cagctaagct ccattccccc gcccattcgt gccgccatgc cgctacccga gctccacgcc
     5341 cccctcttcg tcggcctcgt cctcgtcgtc ctcttcgtcg ccttcgtgcg cgagtgggtg
     5401 aagcccgacg tggcggtcat ggtcgccgtg ggcgtgctgc tcgtggcggg catgctgaac
     5461 accaaggagg tgctcggtgt gttcggcaac agcgccccga tcaccatcgc ctgcctgttc
     5521 atcatcagcg cggcgctgtc gcgcaccggc tgcgtggacc agctcggcga gtggctcgcc
     5581 gccgcggccg gcgcgggcga gcgccgcctg ctgctggcgc tgatcgccgc ctgcctgctg
     5641 gtgtcgccct tcatcaacaa caccccggtg gtgatggtga tgatcccggc ggtgatcgcg
     5701 gtggcggccc gccagggcct cgccccctcg cgcctgctca tcccgctctc ctacgccacc
     5761 atcctcggcg ggctgatcac catggtcggc acctcgacca acatcctggt cgacggcgtc
     5821 gcgcgcgagc agggcctggc gcccttctcg atgttcgaga tcagcctgcc ggcgatcctc
     5881 atggcgctgg tgggcagcgc cttcatgctg gtgttcgcgc cgcgcctgct gccggtgcgc
     5941 gagacgctga gccagcagtt cggcgccggc gagcgctggt tcatgaccga gctcttcgtg
     6001 cccgagggct cgcacctggc cggcaggacg ctgcgcgagg cgcgcctgtc gaacggcatc
     6061 atccaggtgc tcaacctggt gcgcggcgac accgagctcg ccgacccggc cccggacacc
     6121 cggctggagg tcggcgaccg cgtggtcgtg cacagccgca gcgacgcgat gatggcgctg
     6181 cgcagcagcg acggcgtcgg cctgcaggcg ccggcgggcg ccgaggccca cgacatcgag
     6241 accctgcgcc ggcgcgacct ggtgatggtg gaggcgatcg tcggccagac ctcgcgctac
     6301 atcctgcgcc cgatccgcga tctcgacctg ctcgcgcgct acggcatcca cctcgtcgcg
     6361 gtgcatcggc gcgacgcctc gttcgcgcag atcggcgagg acttccagct gctgggcggc
     6421 gacgtgctgc tggtggaggg cacgccggcg cagatccagc gcttctgcga caacggcgac
     6481 ctgttcgcga tcaccgaggg ccgccagatg gccagccgcc gccacaaggc gccggtcgcg
     6541 ctcgccacca tcgtcggcgt gatgctgctc gccgcgctga acgtgatgcc gatcgagggc
     6601 ctggcgctga tcggcgcggc gatcgtgatc gccaccggct gcatcaccag cgacgaggcc
     6661 tacaagtcga tcgagtggcc gatcctggtg ctgatcttcg gcatgctcgc gatcagcatc
     6721 gcgatgcgca actccggcct cgaccagctg ctcgccgccc agctcgccgc gctcggcgag
     6781 ggcatgtcgc cgtggctgat gttgtcgctg gtgatcctga tcacctcggt ggccaccgag
     6841 gtgctgagca acaacgcgat cgcggtgctg ttcaccccgg tcgtgatcgg gctcgcccag
     6901 cagatggggg tggatccgcg ccccttcgtg gtcggcgtga tgttcgccgc aagctgcagc
     6961 ttcgccaccc cgatcggcta ccagaccaac accttggtct atagcgcggg caactaccgc
     7021 ttcgccgact tcgcccgcct cggcgtgccc atgaacatcc tcacctggct gctgtgcagc
     7081 gcgctgatcc cgctgttctg gcccttccag ccctgagccc gcgggccgac atccaggcta
     7141 tccggccgtc accggctgcg gcgcggcttc ggacaagggc ggcggcaggc aggcatgcgc
     7201 gcccgccggc tccaggtgcg ggatcagcaa gggccccatc gacttcagga tctgtaccgg
     7261 caagcccgag gtgaagcggt agctcgccgc ctcggggccg atcacgtagg cggtcagcgt
     7321 gccgaagtgg cgcgggccga ggtagaacac gaaggtggcg gtgcggttga gcgcaagacc
     7381 ggcgcgtccg cgcaccacga tgcggttgtc gccggtgccg gtcttgccac cgagcgccag
     7441 ttcgctgccg tcggccagct tgaaagcgcc cgccagacgg cgcgcggtgc cgccctcgac
     7501 cacgtccgac agcgccccgc gcagcgcgcg cgcgacctcc ggcgccatca ccgggtcgcc
     7561 ttccaccggg cggatggcga aggcggactc gtagggcgtg gcgcgcgcga agcgcaccgt
     7621 gtcgatgcgc tgcacgggca ggcgctggcc gtcgttgacg atgatgccca tcagctcggc
     7681 gagcgccgcc ggacgatcgc ccgagctgcc gagcgcggtg gcgagcgaag gcacgaggtg
     7741 gccgaaggga tagcccaggc gcgcccagcg gcggtggatc tcgaggaagg cctcgacctc
     7801 gagcagggtg aagatgcgcg agtcctgcgc gcccttggcg cgggtgcgga acagccagcg
     7861 atacacctgc tggcgctcgt ccgcgctcgc cgccaccgcc tcggccagcg tggcctcggg
     7921 gtgctgcagg cggaaggcga ccagccacag ctcgagcgga tgcacgcgtg ccacgtagcc
     7981 acggtcgggc aggtcgaagg cttccggggc gttgcgcgca tagagctggg cgaggcgtgc
     8041 ggcggcgggg ggctcgccga ggcggcgggc gaggaaatcg gcgagcgcgt gcggcggggc
     8101 ctcgggctcc aggtagcgga acaccgcggc gaggcggtcc gcgcccggac gcaggccgtc
     8161 gagcagggtg gagcggatct cctccgccgt gctgtcctgg tacttgcgcc agaagcggcg
     8221 caggaagagc tggccctcgc ggtcggcgaa gcggaccaga tactcgacgc ggcgcgggtc
     8281 ctcgttgtcc tcgagcaggc gcgcggtgct gcccggcacc tggtacatgg tgtgacgcac
     8341 gagctcgcgc atcaggcgca cgaagggcag gttgatcgag gcctgcaggg cctcgcgcag
     8401 cgtgggattg cgcgcgttgt cgctggcgtt gaagttgccg aaggtgtggc ggcctccgcc
     8461 ggtgaagaag ccttcgcccg ggctggccga gaagcggcgt tcgagcgcgg cctcgagcat
     8521 cgccggcagg ctgcggtccg gcgcgctggc gaggtggtcg atcgcccagc gcgtcagacg
     8581 gtcctgcgcg tcgacctcga cgcggcgcag cgccggagga tccagcgccg cgagccggcc
     8641 gtggagttcg gcgaccagct cgagataggt cgccaggacg cgcagcttgg cggtggagcc
     8701 gagctcgagc ttgctgccct cgttgatgtc gagcggctgg tcggtggtgt cggtctgcac
     8761 gcgcacgcgg ttgccgcccg cggtgcgctc gaccagcgtg aagctgtagc gtaccgcgcc
     8821 gagggtggca ggctgcagca tgcgctcgcc gatcaggccc tggctacgcg cgaaagccgg
     8881 gtcggagagc tgcccgaggt agtcgcccac cgcctgctgc aggccgccgt gcagggtggt
     8941 gccgagctcg gcgtcgaggc ggtcgaggct gtacagcgag gtgtcgagca ggccggcgag
     9001 ccgggtgcgc accgcggtcg tgcccttgcc gggcgtggcg ggaacccagg ccggatcggc
     9061 atcgaggtcg cgaaaggcga gcggccgggc gagcgcggcg tcgcgcagca cggcgccgat
     9121 cagcccggcc tctgcgaaca ggcgcaggta ggcgtcggtg gtgcgcgcca gctcctcgcg
     9181 cccgccggcg agataccacg agggccgacg gtgcgcgatc atcagcgcga ccacctggcg
     9241 cagcgcccga ccctgggcga tgcgctcctc cccctcgccc accggcgccg ccagcaaggc
     9301 attcacgcgc gcgaactcgg cgccgaacca cacccacagc ccgtcgccca gtccgttgac
     9361 ctcgccgtga ccgggcgcgg ccgagagcgg cacggtgttc aggtagtcga gcaccagccg
     9421 ccggcgtagc ggcagcgtct gctcgccctc gcgataggca cggaccgagg cggacaccat
     9481 ctggcgcagc ttctcgcgcg catcctgggt gatgccgcca ggcgagtggc ggtacttctc
     9541 gatctgggtg gcaagcgtgc tgccgccggg cgcgtcgagg tcggcatcga ccatgcgtcc
     9601 gagctggccg agcgccgcgc gcgcgaagcg cacccagtcc accgcaggat tgagcgtcgg
     9661 ccgcgattcg tcgagcagct cgcggttctc gatgaagagc agggcctggg cgacgatggc
     9721 cggcacgtcc tcgaagcgct cgtatccgcg ccagggatag cggaagccgt acagcggctc
     9781 gccgcgacag tcgaagacgt ccaggcccgc gcgcgtcttc tcggcgtaag gcgggaacag
     9841 gccgcgctcg acgtgggcga gcagcgcatc gtcgaagcgc acctgctcga cgagtgcgaa
     9901 gccgcgcgcc tgcaggcgct cggaaaagcg cgccagctcg gtgtagccga ggcgctggtc
     9961 gaaggggccg tgggcaggaa agcggatcgc cgcgctcggc cccgccagca gggcgtagtc
    10021 gaggcgcgcg gcgtagcgcg cgagctcgcg cgcctgcagg cgcgaggtcc cggcctcgtg
    10081 ccacagcagc aggccgcccg ccacaacagc caccagcacg gcgaccgcga tggcggcagc
    10141 gagcagacgc c
//
LOCUS       DAOQNN010000073        10052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3161_length_10052_cov_9.680004, whole genome shotgun sequence.
ACCESSION   DAOQNN010000073 DAOQNN010000000
VERSION     DAOQNN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3161_length_10052_cov_9.680004"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PKV97_06030"
     CDS             <1..956
                     /locus_tag="PKV97_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB05474.1"
                     /translation="VAWVVVAQGICGVAKDLTKTASKSAIKLTQAQFQAEANEQGAGQ
                     LFKWVAWFTGSKNAMKGIGFFLGGVLLEVLGFRGALWAMAGLLALVLAGVLLALPPMM
                     GRKKASGSVRELFAKSPGINALAAARVALFGARDVWFVVGVPVFLYSAGWTFTMVGTF
                     LAGWTIAYGLVQALAPQIVRRSADGLSREVPAARLWSALLALIPAALAVAVWLQAPGL
                     EWVVVGGLGLFGFAFAVNSSVHSYLVLAYAGSEKAAEDVGFYYAANALGRFIGTLLSG
                     LLYQWGGLLYALVGSAAMLLTCWLVTLALPLERHDAARAAP"
     gene            980..1174
                     /locus_tag="PKV97_06035"
                     /pseudo
     CDS             980..1174
                     /locus_tag="PKV97_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1364..1876
                     /locus_tag="PKV97_06040"
     CDS             1364..1876
                     /locus_tag="PKV97_06040"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNB05475.1"
                     /translation="MSDECYNVLFLCSANSARSIIAEVILNRIGQGRFKACSAGSAPR
                     GEVHPMTLQVLQAQGYDTSALRSKSWDEFSAPDAPQMDFVFTVCDTIAGETCPVWPGQ
                     PLTAHWGVADPVKAEGGEQRRLDAFSLTLNQLLDRLRLFTSLPLATLDRLAVQHEIDR
                     IGQMPAAVEA"
     gene            1878..2942
                     /gene="arsB"
                     /locus_tag="PKV97_06045"
     CDS             1878..2942
                     /gene="arsB"
                     /locus_tag="PKV97_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNB05476.1"
                     /translation="MGLFERYLTVWVGLGILAGVGLGLLAPGAFQAIAGLEFAQVNLV
                     VAVFIWVMIYPMMIQIDWHAVRDVGKKPQGLVLTLVVNWLIKPFTMAALGVLFFQHLF
                     APWVDPAAASEYIAGMILLGVAPCTAMVFVWSQLVKGDPNYTLVQVSVNDLVMVFAFA
                     PIAAFLLGVTDLVVPWETLLLSTVLYVVLPLVAGMATRHVLERRSAQAVAGFVARLKP
                     WSIVGLIATVVLLFGFQARTLVEQPLVIGLIAVPLLVQSYGIFLIAYLAAKVMRLPHE
                     VAGPACLIGTSNFFELAVAVAISLFGLNSGAALATVVGVLVEVPVMLSLVAIVNRTRH
                     WFPSAAGPQAALDRPGGARG"
     gene            2979..3533
                     /locus_tag="PKV97_06050"
     CDS             2979..3533
                     /locus_tag="PKV97_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB05477.1"
                     /translation="MTVPCLLDAWSAHEAELLRYLRHHLRQPGEAEDLLHDLFLKAVR
                     QRERFCAVGNPRAWLFAVARNLVIDNARRTHASVPLPDDLAAPAPDLASEDGPAPVDL
                     LSACLPRVLGELAAEDREAIERCDIGGMTQQAFAELEGLSLSGAKSRVQRARQRLRAQ
                     LLRACQVQLDEHGKVCCFVPRPPL"
     gene            3708..5177
                     /locus_tag="PKV97_06055"
     CDS             3708..5177
                     /locus_tag="PKV97_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNB05478.1"
                     /translation="MDALSSWGLPVFYGGLGNLAAYLAAHVLLCLLPAFFIAGAMSAL
                     IPKETITRFLGRNSSKTVSYPAAAAAGSLLAVCSCTIVPLFAGIYKKGAGLGPAITFL
                     FFAPAANILALVYTGGIIGPDLAVARFLLSLTFGIGIGLIMALLFRADDAAHDLATDS
                     AFGAQGAGMGRMALVFLFVWVALLLAGTLKLDVLTGTYLNFQLPIGDAQGWQAALDRL
                     VPYDAAKGEEGVSVQGVVLIALLASIGYAAWRGFENIVDGANTWTWVSLALIAATLLV
                     AALAVQPVDGGLRVGLTGKFFAVAAALAALAFIVRHRLSEDELRDWLWESWRFVKQIF
                     PLLVVGVFVVGMIRVLIRPEWIEMLAGTNSLTGNLAGVVFGVFMYFPTLVEVPIAKMF
                     LELGMHRGPLLAYLMSDPELSLQSILIISAIIGRRKTFTYVGLVALFSAAAGLIYGAW
                     IDGASLFSLALYLAGFIAVLAVLLAIASRRAAASTLKGV"
     gene            5180..5422
                     /locus_tag="PKV97_06060"
     CDS             5180..5422
                     /locus_tag="PKV97_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB05479.1"
                     /translation="MLDIKVLGPGCANCRKLEEIVHEALAATGVEAEVTKVTDMGQIV
                     AYDVLKTPGLVINEKLVSSGRIPTAATIAEWIRAAG"
     gene            complement(5486..6547)
                     /locus_tag="PKV97_06065"
     CDS             complement(5486..6547)
                     /locus_tag="PKV97_06065"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNB05480.1"
                     /translation="MTVKAYGAHAGDQPLEAMEITRRAPGAHDVQIEIAFCGVCHSDL
                     HQVRSEWPGTLYPCVPGHEIVGRVSAVGAHVKGFKTGELVGVGCIVDSCQHCEDCDAG
                     LENYCDGMIGTYNSPIADAPGHTLGGYSQRIVVHERYVLRIRHPEAQLAAVAPLLCAG
                     ITTWSPLRHWKVGPGQKVGIVGIGGLGHMGVKLAHALGAHVVAFTSSESKRAAALALG
                     ADEVVVSRDEDAMKARRKSLDFILNTVAAPHDLDAYLALLKRDGTMSLVGAPASPHPS
                     PQVFHLIMKRRKLAGSMIGGIPETQEMLDFCAEHGIVADIEMIRAEQIDAAYERMLRG
                     DVKYRFVIDSASLAGEGSA"
     gene            complement(6975..8327)
                     /locus_tag="PKV97_06070"
     CDS             complement(6975..8327)
                     /locus_tag="PKV97_06070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNB05481.1"
                     /translation="MKLAIFGTGYVGLVSGACLAEVGHDVLCMDVDQAKIDQLRAGTI
                     PIWEPGLEPIVARNLAEGRLRFTTDAAEAVRHAEVQFIAVGTPPDEDGSADLQYVLAV
                     AEEIARHMPGYRVIVNKSTVPVGTAGKVQERIAQVLAERGETVAFDVVSNPEFLKEGA
                     AVADFLKPDRILVGTRSARAQERMREIYEPFNRNHERTLFTDVRSAELTKYAANAMLA
                     TRISFMNELANLAERLDADIEEVRKGIGADPRIGYHFIYPGCGYGGSCFPKDVRALGR
                     IAEQVGYDAPLLRAVEAVNERQKNTLFTKLADHFGGAGGLVGRTIAVWGLAFKPNTDD
                     LREAPSRSLIEALWQHGARVQAYDPVAMDEMRRLYGPRADLALASNKYSVLEGADALA
                     ICTEWQQFRAPDFDEMASLLQAKVIADGRNLYPPDRLRAAGWHYYGIGRPERPQAVVR
                     "
     gene            complement(8558..9136)
                     /locus_tag="PKV97_06075"
     CDS             complement(8558..9136)
                     /locus_tag="PKV97_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB05482.1"
                     /translation="MNRIARLTAALLVSAAAASPALAAPQTYVVDGTHTFPSFSYSHF
                     GLSTQLSKFDKTTGTVTLDKEAKTGAVDITIDMKSVNTGYETFDGHIQGEDFLDTAKY
                     PTATFKSTKVSFDGDKPATIDGELTIKGVTKPVTLKVSHFTTMPHPMLKKDTIGANAS
                     TVIKRSEFNAGKYAPHVGDDVTITVSLEAIQN"
     gene            complement(9222..9782)
                     /locus_tag="PKV97_06080"
     CDS             complement(9222..9782)
                     /locus_tag="PKV97_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB05483.1"
                     /translation="MKRIALALLASFAFAATTAHAVEYGEVQPDKSRIAFDYQQMGVA
                     MQGTFGKFASALRFDPAAPQTASARIEVELASVDTGSEEGDDEVARKTWFDTKGFPVA
                     RFESSTVKPLGENRYEVAGKLTIKGTTKDVVVPATFTPQGGSGVFEGALTIRRGDFSI
                     GEGAWKAFDVVANDVVIRFRITAAAR"
     gene            complement(9818..>10052)
                     /locus_tag="PKV97_06085"
     CDS             complement(9818..>10052)
                     /locus_tag="PKV97_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNB05484.1"
                     /translation="GWLMSSAKGFQTVWFGVLPLPDLLAKDKALGNLLETVHVALNFT
                     LIAVLLGHVGAALKHHFIDRDDVLTRMLPRRRG"
BASE COUNT         1321 a         3274 c         3666 g         1791 t
ORIGIN      
        1 cggtggcctg ggtggtggtg gcgcagggca tctgcggcgt cgccaaggac ctgaccaaga
       61 cggccagcaa gtcggcgatc aagctcactc aggcccagtt ccaggccgag gccaacgagc
      121 agggcgcggg ccagctcttc aagtgggtgg cctggttcac cggcagcaag aacgcgatga
      181 agggcatcgg cttcttcctc ggcggggtgc tgctggaggt gctcggcttt cgtggtgcgc
      241 tgtgggcgat ggcgggcttg ctggcgctgg tgctcgctgg cgtgctgctg gcgctgccgc
      301 cgatgatggg caggaagaag gcgtcgggtt cggtgcgcga gctgttcgcc aagagccccg
      361 gcatcaacgc gctggccgcg gcgcgcgtgg cgctgttcgg cgcgcgcgac gtttggttcg
      421 tggtcggggt gccggtgttc ctgtactcgg cggggtggac cttcacgatg gtcggcacct
      481 tcctcgccgg gtggacgatc gcctacggcc tggtgcaggc gctggcgccg cagatcgtgc
      541 gccgcagtgc cgatggcctg agccgcgagg tgccggcggc acggctgtgg tcggcgctgc
      601 tggcgctgat tcccgccgcg ctcgcggtgg cggtgtggct gcaggcgccc ggcctggagt
      661 gggtggtggt cggcggcctc ggactgttcg gcttcgcctt cgcggtcaac tcctcggtgc
      721 attcctacct ggtgctggcc tacgcgggct cggagaaggc cgcggaggac gtcggtttct
      781 attacgctgc caacgcgctc gggcgcttca tcggcacgct gctgtcggga ctgctgtacc
      841 agtggggcgg cttgctgtac gcgctcgttg gctcggcggc gatgctgctg acgtgctggc
      901 tggtgacgct ggcgttgccg ctcgaacgcc atgacgccgc ccgggcggcc ccctgactcc
      961 gcttgacgaa gagatccgaa tggacaagaa agccgcagtt tccgccttcg aggcgctgtc
     1021 ctcgggcatc cgcctggatg tgttccgcct gctggtgcgt gccgggccgc agggcagggt
     1081 ggccggcgag ctcgccagcg cgctcgagct gccgccgacc aacctgtcct tccacctcaa
     1141 ggcgctcgcg caggcggggc tggtctcggc cgaacaggaa gggcgcttcc agcgttatcg
     1201 cgccgacatg gcgcggatgc gcgggctgat cgcctacctc acagaggaat gctgcggcgg
     1261 tgcgcccgca ctgtgcgggc tggatggcag cgcttccatg accggattca ccacgcccgc
     1321 cgcgcgccac gcggcgggcc agacacgacg accggagtct tccatgtccg acgagtgcta
     1381 caacgtgctc ttcctgtgct cggccaactc ggcgcgcagc atcatcgccg aggtcatcct
     1441 caaccggatc ggccagggcc gcttcaaggc ctgcagcgca ggcagcgcgc cgcgcggcga
     1501 ggtgcacccg atgaccctgc aggtgctgca ggcgcagggc tacgacacga gcgcgctgcg
     1561 cagcaagagc tgggacgagt tttccgcgcc ggatgcgccg cagatggact tcgtgtttac
     1621 cgtgtgcgac acgatcgccg gcgagacctg cccggtgtgg ccgggccagc cgctgaccgc
     1681 gcactggggt gtggccgatc cggtgaaggc cgagggcggc gagcagcgcc ggctcgacgc
     1741 cttcagcctg acgctgaacc agctcctcga tcgtctgcgc ctcttcacca gcctgccgct
     1801 ggccacgctc gaccgcctcg cggtccagca cgagatcgac cgcatcggcc agatgccggc
     1861 cgcagtggag gcctgagatg ggactcttcg agcgctacct caccgtctgg gtggggctgg
     1921 gcatcctcgc cggggtgggc ctcgggctgc tcgcgcccgg ggccttccag gccatcgccg
     1981 ggctcgagtt cgcgcaggtg aacctggtgg tggcggtgtt catctgggtg atgatctacc
     2041 cgatgatgat ccagatcgac tggcacgccg ttcgcgatgt cggcaagaag ccgcagggac
     2101 tcgtcctgac cctggtcgtg aactggctga tcaagccctt cacgatggcg gcgctcggcg
     2161 tgctgttctt ccagcacctg ttcgcgccct gggtggatcc ggctgcggcc agcgagtaca
     2221 tcgccggcat gatcctgctc ggcgtggcgc cgtgcacggc gatggtgttc gtatggagcc
     2281 agctggtgaa gggcgacccc aactacaccc tggtgcaggt gtcggtgaac gatctcgtca
     2341 tggtgttcgc cttcgccccc atcgccgcct tcctgctcgg cgtcaccgat ctcgtcgtgc
     2401 cgtgggagac cctgctgctg tcgaccgtgc tctacgtggt gctgccgctc gtggccggca
     2461 tggcgacgcg gcatgtgctg gagcggcgct cggcgcaggc ggtggccggg ttcgtcgctc
     2521 gcctcaagcc ctggtccatc gtcggcctga tcgccaccgt ggtgctgctg ttcggtttcc
     2581 aggcgcgcac cctcgtggaa cagccgctgg tgatcggcct gatcgccgtg ccgctgctgg
     2641 tgcagagcta cggcatcttc ctcatcgcct atctcgccgc gaaggtgatg aggctgccgc
     2701 acgaggtcgc cggcccggcc tgcctgatcg gcacctcgaa tttcttcgag ctcgccgtgg
     2761 cggtggcgat ctcgctgttc gggctgaatt cgggtgcggc gctggcgacc gtggtcggtg
     2821 tgctggtgga ggtgccggtg atgctgtcgc tggtggcgat cgtcaatcga acgcggcatt
     2881 ggtttccgtc cgcggcaggg ccgcaggcgg ctctcgaccg accgggtggc gcgcgtggct
     2941 gagcgaggcg tcgagccgcc gtgcacgggc gcgccgacgt gaccgtcccc tgcctgctcg
     3001 acgcctggtc ggcgcacgag gccgagctcc tgcgctacct gcgccatcac ctccgccagc
     3061 cgggagaggc cgaggacctg ctccacgacc tcttcctcaa ggcggtgcgc cagcgcgagc
     3121 gcttctgcgc cgtcggcaac cctcgtgcct ggctctttgc ggtggcgcgc aacctggtga
     3181 tcgacaacgc gcgacgcacg cacgcgagcg tgcccctgcc ggacgatctc gccgcgccgg
     3241 cgcccgacct cgcgagcgag gacggcccgg cgccggtcga cctgctctcg gcctgcctgc
     3301 cgcgcgtcct cggcgagctc gccgcggagg accgcgaggc gatcgagcgc tgcgacatcg
     3361 gcgggatgac gcagcaggct ttcgccgaac tcgagggcct gtcgctgtcg ggcgccaagt
     3421 cgcgcgtgca gcgcgcacgg cagaggctgc gcgcgcagct gctgcgcgcc tgccaggtgc
     3481 agctcgacga gcacgggaag gtgtgctgct tcgtgccgcg cccgccgctc tagcccccgc
     3541 ctgcatcccc cgcctgcatc ccgcacctgc gcctgcatcc ttttcaccac tcgtgcgtct
     3601 tcatggttgg agccgcttgc cggcgaccgc gcaggcgact aggtttcgtc tttaggtcga
     3661 tccgatcgca tggcgggatg caggtttctc gagctggagt tcgtgtcatg gatgcgttgt
     3721 cttcctgggg cctccccgtc ttctatggcg gcttgggcaa tctggccgcc tatcttgcgg
     3781 cgcatgtgct gctgtgcctg ctgccggcct tcttcatcgc cggcgcgatg tcggcgctga
     3841 tcccgaagga gaccatcacc cgcttcctcg ggcgcaactc cagcaagacc gtgtcctacc
     3901 cggcggctgc cgcggcgggc tcgctgctgg cggtgtgctc gtgcaccatc gttccgctct
     3961 ttgccggcat ctacaagaag ggcgcgggcc tcggcccggc gatcaccttc ctgttcttcg
     4021 ccccggccgc caacatcctc gcgctggtct acaccggcgg catcatcggg cccgacctcg
     4081 ccgtcgcgcg cttcctgctg tcgctcacct tcggcatcgg catcggcctg atcatggcgc
     4141 tgctcttccg tgccgacgac gccgcgcacg acctcgccac cgacagcgcc ttcggcgcgc
     4201 agggggcggg gatggggcgg atggcgctgg tgttcctgtt cgtgtgggtg gcactgctgc
     4261 tggccggcac gctgaagctg gacgtgctga ccgggaccta cctgaacttc cagcttccga
     4321 tcggtgacgc gcagggctgg caggccgcgc tcgaccgcct ggtgccctac gacgccgcca
     4381 agggcgagga gggcgtgtcg gtgcagggcg tggtgttgat cgcgctgctg gcctcgatcg
     4441 gctatgccgc ctggcggggc ttcgagaaca tcgtcgacgg cgccaacacg tggacctggg
     4501 tcagcctcgc gctgatcgcg gcaacgctgc tggtggcggc gctggcggtg cagccggtgg
     4561 acggcgggct gcgcgtcggc ctcaccggca agttcttcgc cgtcgccgcc gcgctcgcgg
     4621 cgctggcttt catcgtgcgc catcgcctga gcgaggacga actgcgcgac tggctgtggg
     4681 aatcctggcg cttcgtgaag cagatctttc cgctgctggt ggtcggcgtg ttcgtggtcg
     4741 gcatgatccg cgtgctgatc cgcccggagt ggatcgagat gctggccggc acgaactcgc
     4801 tcaccggcaa cctggccggc gtggtgttcg gcgtgttcat gtacttcccg accctggtcg
     4861 aggtgccgat cgccaagatg ttcctcgaac tcggcatgca ccgcggcccg ctgctcgcct
     4921 acctgatgtc cgaccccgag ctgtcgctgc agagcatcct gatcatctcg gcgatcatcg
     4981 gccgcaggaa gaccttcacc tacgtcggcc tggtggcgct gttcagcgcc gccgccggcc
     5041 tcatctacgg cgcctggatc gacggcgcct ccctgttctc gctcgcgctc tacctggcgg
     5101 gcttcatcgc cgtgcttgcg gtgttgctgg cgatcgccag ccggcgcgcg gccgcctcta
     5161 ccctcaaagg agtctgatca tgctcgacat caaggttctc ggcccgggct gcgccaactg
     5221 ccgcaagctc gaagagatcg tgcacgaggc cctcgccgcc accggcgtgg aggcggaagt
     5281 caccaaggtc accgatatgg ggcagatcgt cgcctacgac gtgctgaaga cgcccgggct
     5341 ggtgatcaac gagaagctgg tgtcgtcggg gaggattccg accgcggcga cgatcgcgga
     5401 gtggatccgc gcggcggggt aggcgccgtg ctcgacatgc cgtaacgcgt cgagcacggc
     5461 ctcttcagcg gatgagcgcg gccgctcagg cggagccttc ccccgccagg ctcgcgctgt
     5521 cgatcacgaa gcggtacttc acgtcgcccc tcagcatgcg ctcgtaggcc gcgtcgatct
     5581 gttcggcgcg gatcatctcg atgtcggcga cgatgccgtg ctcggcgcag aagtcgagca
     5641 tctcctgggt ctcggggatg ccgccgatca tcgagccggc gagcttgcgg cgcttcatga
     5701 tgaggtggaa gacctgtggc gagggatggg gcgaggccgg tgcgccaacc aggctcatcg
     5761 tgccgtcgcg cttgagcagc gcgaggtagg cgtcgaggtc gtggggggcg gcgacggtgt
     5821 tcaggatgaa gtccagcgac ttgcgccgcg ccttcatcgc gtcctcgtcg cgtgacacca
     5881 cgacctcgtc ggcgccgagc gcgagcgcgg ctgcacgctt ggactcggag ctggtgaagg
     5941 cgaccacgtg cgcgccgagc gcgtgggcga gcttgacccc catgtggccc agcccgccga
     6001 tgccgacgat gcccaccttc tgcccggggc cgaccttcca gtggcgcagc ggcgaccagg
     6061 tggtgatgcc tgcgcacagc agcggcgcca ccgcggcaag ctgggcctcg gggtggcgga
     6121 tgcgcagcac gtagcgctcg tgcaccacga tgcgctgcga gtagccgccc agggtgtggc
     6181 cgggggcgtc ggcaatgggg ctgttgtagg tgccgatcat gccgtcgcag tagttctcca
     6241 ggccggcgtc gcagtcctcg cagtgctggc agctgtcgac gatgcagccc acgccgacga
     6301 gctcgccggt cttgaagccc ttcacgtgtg cgcccaccgc cgacacgcgg ccgacgatct
     6361 cgtggccggg cacgcagggg tagagcgtgc ccggccactc cgagcgcacc tggtggaggt
     6421 cggagtggca tacgccgcag aaggcgatct cgatctgcac gtcgtgcgcg ccgggcgcgc
     6481 ggcgggtgat ctccatggct tcgaggggct ggtcgccggc atgggcgcca taggctttga
     6541 cggtcatcgg gggggcctcg ggttgggttg cgggatgata accagagaaa gccttccggc
     6601 tccggtgttg tccccagccg atggggaaag gcatgtgggc ggggctgtgg acaaccgcgc
     6661 gcaagccgcg aggcacaagg gttgcgggcg attgcctcat tgtgaggcag aaggcccccg
     6721 ccacctccgg cgggcccgac ggttcttgcc ggagcgaggg gaggcgggat cgtggcttgc
     6781 agcctgcgcg atcgttggcc cgcacgccgt atcgcgactc gcgtcgctcc cacagccagt
     6841 gccagccgtg cctggtggcc cggcggttct gcaggagcga cgcaagtcgc gatgcccacg
     6901 tctcccagcc gtgcccggtg gcccggcggt tcggttctgc aggagcgacg caagtcgcga
     6961 tgtccacgtc tgttctaccg gaccacggct tgggggcgct cgggtcggcc gatgccgtag
     7021 tagtgccagc cggcggcgcg caggcggtcc ggtgggtaca ggttgcgccc gtcggcgatg
     7081 accttggcct gaagcaggga cgccatctcg tcgaaatcgg gcgcgcggaa ctgctgccac
     7141 tcggtgcaga tcgccagcgc gtccgcgcct tcgagcacgc tgtacttgtt gctcgccagc
     7201 gccaggtcgg cgcgcgggcc atagagccgc cgcatctcgt ccatcgccac cgggtcgtag
     7261 gcctgcacgc gcgcgccgtg ttgccacagc gcctcgatca ggctgcggct gggggcttcg
     7321 cgcaggtcgt cggtgttggg cttgaaggcg aggccccaga ccgcgatcgt gcgtccgacg
     7381 agtccgccgg cgccgccgaa gtggtcggcg agcttggtga acaaggtgtt cttctggcgc
     7441 tcgttcacgg cctccaccgc ccgcagcagt ggcgcgtcgt agcctacctg ctcggcgatg
     7501 cgaccgagcg cccggacgtc cttggggaag cacgagccgc catagccgca gccggggtag
     7561 atgaagtggt agccgatgcg cgggtcggcg ccgatgccct tgcgcacctc ctcgatgtcg
     7621 gcgtcgaggc gctcggcgag gttggcgagc tcgttcatga agctgatgcg ggtggcgagc
     7681 atcgcattgg cggcgtactt ggtgagctcg gcgctgcgca cgtcggtgaa gagggtgcgc
     7741 tcgtggttgc ggttgaaggg ctcgtagatc tcgcgcatgc gctcctgcgc gcgcgccgag
     7801 cgggtgccga caaggatgcg gtcgggcttg aggaagtcgg cgaccgccgc gccctccttg
     7861 aggaattcgg ggttggagac gacgtcgaag gcgaccgtct cgccgcgctc ggcgagtacc
     7921 tgcgcgatgc gctcctgcac cttcccggcg gtgccgacgg gtacggtgga tttgttcacg
     7981 atcacccggt agcccggcat gtgacgggcg atctcctcgg cgaccgcgag cacgtactgc
     8041 aggtcggccg agccgtcctc gtcgggcggg gtgccgacgg cgatgaactg cacctcggcg
     8101 tggcgcaccg cctcggcggc gtctgtggtg aaacgcaggc ggccctcggc gaggttgcgc
     8161 gcgacgatcg gctccaggcc gggctcccag atcgggatgg tgccggcgcg cagctggtcg
     8221 atcttggcct ggtcgacgtc catgcacagc acgtcgtggc cgacttccgc gaggcacgcg
     8281 ccgctgacca ggccgacgta gccggtaccg aaaatggcga gcttcatgaa ggacatccct
     8341 gtttgcgcga atgcgccaga agctagggag tccgggtgaa gcggtcgtga agcttgcgtg
     8401 aaggggaggt tgcgaagcgc cccgggagcg cgtcggtcgc cgcatcggcg gattcgcggc
     8461 ttgcgccgct cctacaaagg atgcgtcgag ttacgcggga atgccaggcg cggattctgc
     8521 ggccgagctc gggagccgca tcggccgcgc ctgcagctta gttctggatg gcttccagcg
     8581 agacggtgat ggtcacgtcg tcgcccacgt gcggggcata cttgccggcg ttgaactcgc
     8641 tgcgcttgat cacggtgctg gcgttggcgc cgatggtgtc cttcttgagc atcgggtgcg
     8701 gcatggtggt gaagtggctc accttcagcg tcaccggctt ggtcacgccc ttgatggtca
     8761 gctcgccgtc gatggtcgcg ggtttgtcgc cgtcgaagct caccttggtc gacttgaagg
     8821 tggccgtcgg gtacttggca gtgtcgagga agtcctcgcc ctggatgtgg ccgtcgaagg
     8881 tttcgtagcc ggtgttcacc gacttcatgt cgatggtgat gtcgaccgcg ccggtcttcg
     8941 cttccttgtc cagcgtcacg gtgccggtcg tcttgtcgaa cttggagagc tgggtcgaca
     9001 ggccgaagtg gctgtacgag aagctcggga aggtgtgggt gccatcgacg acgtaggtct
     9061 ggggcgcggc cagcgcgggc gaggcggcag cggcggagac gaggagggcg gcggtgaggc
     9121 gggcgatacg gttcatgtgg tggtcctttg tggagggcag atgggtggaa cggaagggtt
     9181 ggaacgatgg cgcgggaacg atgctgcccg gctgccgcgg ctcagcgtgc cgcggcggtg
     9241 atgcggaagc ggatcacgac gtcgttggcg accacgtcga aggccttcca ggcgccttcg
     9301 ccgatcgaga agtcgccgcg ccggatggtc agggcgccct cgaagacgcc gctgccgccc
     9361 tgcggggtga aggtggcggg gaccacgacg tccttcgtcg tgcccttgat cgtgagcttg
     9421 ccggcgacct cgtagcggtt ttcgccgagc ggtttcaccg tgctcgactc gaagcgcgcg
     9481 accgggaagc ccttggtgtc gaaccaggtc ttgcgggcga cctcgtcgtc gccttcctcg
     9541 ctgccggtgt cgacgctggc gagctcgacc tcgatgcggg ccgaggcggt ttgtggcgcg
     9601 gcgggatcga agcgcagcgc gctcgcgaac ttgccgaagg tgccctgcat cgccaccccc
     9661 atctgttggt agtcgaaggc gatgcggctc ttgtcgggct ggacctcgcc gtattccacc
     9721 gcgtgggcgg tggtcgcggc gaaggcgaag gaggcgagca gggcgagggc gatgcgtttc
     9781 atggcgcggg ttcctgtgtg cgatggagga ggccggttca gccccggcgg cgcggcagca
     9841 tgcgggtcag cacgtcgtcg cggtcgatga agtgatgctt gagcgcggcg ccgacgtggc
     9901 cgagcagtac ggcgatcagc gtgaagttga gcgccacgtg cacggtctcg agcagattgc
     9961 cgagcgcctt gtccttggcg agcaggtcgg gcagcggcag cacgccgaac cacaccgtct
    10021 ggaagccctt ggccgagctc atcagccagc cg
//
LOCUS       DAOQNN010000074        10026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3177_length_10026_cov_8.649885, whole genome shotgun sequence.
ACCESSION   DAOQNN010000074 DAOQNN010000000
VERSION     DAOQNN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10026
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3177_length_10026_cov_8.649885"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PKV97_06090"
     CDS             <1..413
                     /locus_tag="PKV97_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HNB05485.1"
                     /translation="HTLYFGRHSVQGPVTEADWQAFVDAEVTPRFMDGLTAWAARRQW
                     RDIHTARIVREDSVVLQLVVPAHKDARGALRDIVAAYKARFDDLRGDVLAAQGKVAEA
                     RAAYQAAIDALAAVGDDAVTLREVVRVKLESLGA"
     gene            417..1559
                     /gene="bamB"
                     /locus_tag="PKV97_06095"
     CDS             417..1559
                     /gene="bamB"
                     /locus_tag="PKV97_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HNB05486.1"
                     /translation="MKTPSLRLVPLLVAALLAAGCSSLNPFASSAPKPAPLVDFKPGA
                     QLVAKWQANIGEAEHYRFQPAVWSDSVYAAGNEGEVARFEDGRTVWRIDTGGELSSGV
                     GTDGRLAVVVATDGTVIALDTDNGSERWRAPIGAEVLAPPAVSLELVIVRASDNRLIA
                     LSARDGSRRWVYQRNNPPLALRSFAGVVLEGPVVLAGFPGGKLAVINAANGGAITELN
                     VAVPRGATELERVADVAGVPVVGRREVCAVAYQGRAACFDTSNGNAIWSREFSSSVGM
                     DRDARFALITDDRDAVHALDAASGASVWKQDALARRHVSRPLIVGDYVIVGDLEGYVH
                     ALARETGAFAARERAGSDPIVADPVPLRRGFVVQGAGGDITAYEVR"
     gene            1573..2901
                     /gene="der"
                     /locus_tag="PKV97_06100"
     CDS             1573..2901
                     /gene="der"
                     /locus_tag="PKV97_06100"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNB05487.1"
                     /translation="MKPTIVLVGRPNVGKSTLFNRLTRTRDALVADQPGLTRDRHYGI
                     GRVGDRDYLVVDTAGFDPVAKDGIMHEMARQAEQAIAEADVLLFLVDGRAGRTPHDEN
                     IAARLRRAGRPVHVVVNKAEGIDRAMVAADFHALGLGDPLPVSAAHGDGVKQLVELVL
                     APFPADDEKEHSDDEGPKVAIVGRPNVGKSTLVNTLLGEERVIAFDMPGTTRDAIAIP
                     FERGGKRYTLIDTAGLRRRGKVFEAVEKFSVIKTLQAVQECNVVVLVLDAAQDISDQD
                     AHIAGFALEAGRALVVAINKWDAVDDYRRDRLKADIARKLAFLSFARFHQISALKAEG
                     IAGLLKSVDAAYAAATANLATPRLTRALQQAVARQAPPRHGLARPKMRYAHQGGMNPP
                     VIVIHGNALDDIPQSYVRYLERCFMDAFDLKGTPLRIQFRTTHNPYATRT"
     gene            3016..3252
                     /gene="hfq"
                     /locus_tag="PKV97_06105"
     CDS             3016..3252
                     /gene="hfq"
                     /locus_tag="PKV97_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNB05488.1"
                     /translation="MSNKGQLLQDPFLNTLRREHIPVSIYLVNGIKLQGQIESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVVIQQEEGAN"
     gene            3307..4464
                     /gene="hflX"
                     /locus_tag="PKV97_06110"
     CDS             3307..4464
                     /gene="hflX"
                     /locus_tag="PKV97_06110"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNB05489.1"
                     /translation="MFERPASGERAVLVQLDLGQGAIDERLSELKLLVLSAGASVEAV
                     VQGRRAAPDSKLFAGSGKVQEIGEALRAYGADIVIFNHALSPGQQRNLERELQCMVID
                     RTALILDIFAQRARSHEGKLQVELAQLEHLATRLVRGWTHLERQKGGIGLRGPGEKQL
                     ETDRRLLGNRVKMLKSRLAQIEKQRKVRRRARERRDVLSVSLVGYTNAGKSTLFNALT
                     KAGAYAADQLFATLDTTSRRLYVGGEGAGASVVLSDTVGFIRDLPHALVAAFQATLEE
                     TAQADLLLHVVDSASEDRDAQIGAVNQVLAEIGAADVPQILVWNKIDLTRAAAAVERG
                     DCGTIRRIFLSARTGEGLDLLRDALAEVARQTFGDNADRPAGRDDERRQET"
     gene            4479..5780
                     /gene="hflK"
                     /locus_tag="PKV97_06115"
     CDS             4479..5780
                     /gene="hflK"
                     /locus_tag="PKV97_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNB05490.1"
                     /translation="MSLNDPRWGGQGGGDREDGNRGDDNRNDDQRGDRNRGGNQGPPD
                     LEEVWHDFNQRLSGMFGGKRPGRGGGFGGGNGGGNRPELPGFSFKQFRGGFGVLAALV
                     LVVWLASGLYTVDANQRAVVLRLGEYVATTEPGLRWRLPAPFETHEIVDLTGVRTVEV
                     GYRGSERNKVLRESLMLTDDENIINIQFAVQYVLNSPENYIFNNRFPDEAVAQAAETA
                     MREIVGKSRMDFVLYEGREEIATTAHELMQRILDRYETGIQVSRVTMQNAQPPEQVQA
                     AFDDAVKAGQDRERQKNEGEAYANDVVPRARGTASRLVEEANAYRERVVANAEGEASR
                     FSQVFAEYNRAPEVTRERLYLDTMQQVMSSTSKVMVDAKGNGNLLMLPLDKLMQQTGA
                     KPAAAAPPDSQSAAGLAANAPVPSFDPRNRDGMFSRERGVR"
     gene            5780..6661
                     /gene="hflC"
                     /locus_tag="PKV97_06120"
     CDS             5780..6661
                     /gene="hflC"
                     /locus_tag="PKV97_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNB05491.1"
                     /translation="MRDKMSLIGGTLLLLVVIASMSLFTVDQRQYAIVFQLGEVKEVI
                     SEPGLNAKLPFIQNVRYFDKRILTMDTPEPERFITSEKKNVLVDHFVKWRIVDPRLYY
                     ESVAGDEARARTRLTQTVNAGLREEFGRRTVHDVVSGERDRIMEQMRERADRDARTIG
                     VQIVDVRLKRVDLPNEVSESVYRRMEAERKRVANELRSLGAAEAERIRADADRQREVI
                     IAEAYRSAQEVKGAGDAKATAIYAEAFGKDREFYSFYRSLEAYRASFSGKDDVLVVDP
                     SSDFFRFMKDAGGAPRN"
     gene            6669..6854
                     /locus_tag="PKV97_06125"
     CDS             6669..6854
                     /locus_tag="PKV97_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HNB05492.1"
                     /translation="MASSLLTAFALMLIVEGLLPFIAPAAWRETFLRLASMADGQIRF
                     IGLSSMLAGLAMLFIVL"
     gene            6862..8019
                     /locus_tag="PKV97_06130"
     CDS             6862..8019
                     /locus_tag="PKV97_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNB05493.1"
                     /translation="MRWVLPDHIQDALPSDAHRLESLRRRLLDAFRLRGYQLVMPPLL
                     EYLDSLTTGAGKDLELRTFKLVDQLSGRSMGVRADMTPQVTRIDAHLLNRRGVSRLCY
                     CGSVLHTLPSTLTGTREPLQLGAELYGHAGIEADVEILRLLAEVLRLAEVPASRIDIG
                     HVGLFHALAARAGLVPGREEELFDLLQAKDVPELRRMLADVSEPARSALLALPELYGG
                     PEVLAEAAARLPQDAEIAGPLAELRQLADALGDLPVSFDLADLRGYHYHSGVVFAAYG
                     AESPAALALGGRYDRVGQAFGRARPATGFSLDLRELAWHLPTPAAPAGAVLAPPDDDA
                     GLTSEVSALRARGEIVMVALPGHEGTWNEAGCDRQLVKRGDRWAVVPLQGE"
     gene            8024..9334
                     /locus_tag="PKV97_06135"
     CDS             8024..9334
                     /locus_tag="PKV97_06135"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNB05494.1"
                     /translation="MAKNVVVVGTQWGDEGKGKIVDWLTDHAKGVVRFQGGHNAGHTL
                     VVGKTEYKLNLVPSGIVREGVACFIGNGVVLDAHHLLSEIRTLEAGGIKVRDRLRVSP
                     GCPLILGYHAALDRAREAAKSAGDKIGTTGKGIGPTYEDKVARRALRVYDLFDRERFA
                     AKLKANLEYHNFVLTRHLGAEPVDFQTVFDQAMADADELLPMVADVSAELYAINKSGG
                     SLLFEGAQGTLLDIDHGTYPFVTSSNCVAGQAAAGSGVGPGRLHYVLGITKAYCTRVG
                     GGPFPTELDIETKGVPGEQMSTKGREFGTVTGRKRRCGWLDLAALKRSIIINGVTGLC
                     ITKLDVLDGLEELKLCTGYMLDGRRIDLLPMGSEEVTRCEPIYETLKGWSGTTFGAQS
                     WDALPQEARTYLHRIEEICEIPIDVISTGPERDETILRRHPFGA"
     gene            complement(9634..9718)
                     /locus_tag="PKV97_06140"
     tRNA            complement(9634..9718)
                     /locus_tag="PKV97_06140"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9682..9684),aa:Leu,seq:cag)
BASE COUNT         1626 a         3627 c         3257 g         1516 t
ORIGIN      
        1 tgcacacgct gtacttcggc cgccacagcg tccagggccc ggtgaccgag gccgactggc
       61 aggccttcgt cgacgccgag gtcacgccgc gcttcatgga cggcctcacg gcctgggccg
      121 cgcgccgcca gtggcgcgac atccacaccg cccgcatcgt gcgcgaagac agcgtcgtgc
      181 tgcagttggt ggtgcccgcg cacaaggacg cccgcggcgc gctgcgtgac atcgtggccg
      241 cctacaaggc gcgcttcgac gacctgcgcg gcgacgtgct cgccgcccaa ggcaaggtcg
      301 cggaggcgcg cgccgcctac caggccgcga tcgacgcgct cgccgccgtc ggtgacgatg
      361 cggtcacgtt gcgcgaagtc gtccgcgtca aactcgaatc cctcggagcc tgagcgatga
      421 aaaccccctc gctgcgtctc gtcccgctgc tcgtcgccgc gctgctcgcc gcgggttgct
      481 cctcgctcaa ccccttcgcc agctccgcac ccaagcccgc gccgctggtc gacttcaagc
      541 ccggcgcgca gctcgtggcc aaatggcagg cgaacatcgg cgaggccgaa cactaccgct
      601 tccagcccgc ggtctggagc gacagcgtgt acgccgccgg caacgagggc gaggtcgcgc
      661 gcttcgagga cggccgcacg gtgtggcgga tcgataccgg cggcgaactc tcctccgggg
      721 tcggcaccga tggccgcctc gcggtggtgg tcgccaccga cggcacggtg atcgccctcg
      781 acaccgacaa cggcagcgag cgctggcgcg cgccgatcgg cgccgaggtg ctggcgccgc
      841 ccgcggtcag cctcgagctg gtgatcgtgc gcgcctccga caaccgcctg atcgcgctca
      901 gcgcgcgcga cggcagccgg cgctgggtgt accagcgcaa caacccgccg ctcgcgctgc
      961 gcagcttcgc cggcgtggtc ctcgaaggcc cggtcgtgct cgccggcttc cctggcggca
     1021 agctcgccgt gatcaacgct gccaatggcg gcgcgatcac cgagctcaac gtcgccgtgc
     1081 cgcgcggagc gaccgaactc gaacgcgtcg ccgacgtggc cggcgtgccg gtggtcgggc
     1141 gtcgcgaggt ctgcgcggtc gcctatcagg gccgcgccgc ctgcttcgac acctcgaacg
     1201 gcaacgcgat ctggtcgcgc gagttctcca gcagcgtcgg catggaccgc gacgcgcgct
     1261 tcgcgctcat caccgacgac cgcgatgcgg tccatgcgct cgacgccgcc agcggcgcca
     1321 gcgtgtggaa gcaggacgcg ctcgcgcgcc gccacgtctc ccgtcccctg atcgtcggcg
     1381 actacgtgat cgtcggcgac ctcgagggct atgtgcatgc gctcgcgcgc gagaccggcg
     1441 ccttcgccgc gcgcgagcgc gccggcagtg atccgatcgt ggccgatccg gtcccgctgc
     1501 gccgcggttt cgtggtgcag ggcgccggcg gcgacatcac cgcgtacgaa gtacgctgag
     1561 gtacggtagc cagtgaaacc caccatcgtt ttggtcggcc ggcccaatgt cggcaagtcg
     1621 accctgttca accgcctgac gcgcacccgc gacgcgctcg tcgccgacca gcccggcctc
     1681 acccgcgacc gccactacgg catcggccgc gtcggcgacc gcgactatct ggtcgtcgat
     1741 accgccggct tcgaccccgt ggccaaggac ggcatcatgc acgagatggc gcgccaggcc
     1801 gagcaggcca tcgccgaggc cgacgtgctg ctgttcctgg tcgacggccg cgccgggcgc
     1861 accccgcacg acgagaacat cgccgcgcgc ctgcgccgtg ccgggcggcc ggtgcatgtg
     1921 gtcgtcaaca aggccgaggg tatcgaccgc gcgatggtcg ccgccgactt ccatgcgctc
     1981 ggcctcggcg atccgctgcc ggtctcggcg gcgcacggcg acggcgtcaa gcagttggtc
     2041 gaactcgtgc tagcgccctt cccggccgac gacgagaagg agcactccga cgacgagggc
     2101 cccaaggtcg ccatcgtcgg ccgtcccaac gtcggcaaat ccaccttggt gaacaccctg
     2161 ctcggcgaag agcgcgtcat cgccttcgac atgccgggta ccacccgcga cgccatcgcg
     2221 atccccttcg agcgcggtgg caagcgctac accctgatcg acaccgccgg cctgcgccgc
     2281 cgcggcaagg tcttcgaggc ggtggaaaag ttctccgtca tcaagaccct gcaggccgta
     2341 caggagtgca acgtggtcgt gctggtgctc gacgccgcgc aggacatctc cgaccaggac
     2401 gcccacatcg ccggcttcgc gctcgaggcc gggcgcgcgc tggtggtggc gatcaacaag
     2461 tgggatgcgg tcgacgacta ccgccgcgat cgtctcaagg ccgacatcgc gcgcaagctg
     2521 gccttcctct cgttcgcgcg cttccaccag atctccgcgc tcaaggccga gggcatcgca
     2581 gggctgctga agtcggtgga tgccgcctat gccgcggcca ccgccaacct ggccacgccg
     2641 cggctgacgc gtgcgctgca gcaggcggtc gcgcgccagg cgccaccgcg ccacgggctg
     2701 gcgcgcccga agatgcgcta cgcccaccag ggcggcatga accctccggt gatcgtcatc
     2761 cacggcaacg cgctggacga catcccgcag tcgtatgtgc gctacctgga gcgctgtttc
     2821 atggatgcct tcgacctcaa gggcacgccc ttgcgcatcc agttccgcac cacgcacaat
     2881 ccttacgcga cgcggacctg agcatttttc gcgaaagaac ccgtttccac gcctggattt
     2941 cgttgcatac tgccatgcgg cagtgcacaa cgtgtctgca atattcttat aaacacaata
     3001 taaagaggtt ccacaatgag caacaaaggg caactcctcc aggacccctt cctgaacacc
     3061 cttcgtcgcg aacacattcc cgtgtcgatc tacctggtca acggcatcaa gctgcagggc
     3121 cagatcgagt ccttcgacca atacgtcgtg ctgctcaaga acaccgtcac ccagatggtc
     3181 tacaagcacg cgatctccac cgtcgtgccc gcccgcccgg tcgtcatcca gcaggaagag
     3241 ggtgccaact gaggccgcgg tccacccggc gcggccggcg tctgccggct gcgggaggcg
     3301 cgcgtcgtgt ttgaacgtcc cgcctccggt gagcgtgcgg tcctcgtgca gctcgacctc
     3361 gggcagggcg cgatcgacga gcgcctgtcc gagctgaagc tgctcgtcct cagcgccggc
     3421 gcgagcgtcg aagcggtcgt gcaaggccgc cgcgccgcgc ccgattccaa gctcttcgcc
     3481 ggcagcggca aggtgcagga gatcggcgag gcgctgcgcg cctacggcgc cgacatcgtc
     3541 atcttcaacc acgccttgtc gcccgggcag cagcgcaacc tcgagcgcga actgcagtgc
     3601 atggtcatcg accgcaccgc gctgatcctc gacatcttcg cgcagcgcgc gcgcagccac
     3661 gaaggcaagc tgcaggtcga actcgcccag ctcgagcacc tggcgacccg cctcgtgcgc
     3721 ggctggaccc acctcgagcg ccagaagggc ggcatcggcc tgcgcggccc gggcgagaag
     3781 cagctcgaga cggaccgccg cctgctcggc aaccgagtca agatgctcaa atcccggctc
     3841 gcccagatcg agaagcagcg caaggttcgt cggcgcgctc gggaacgccg cgacgttctg
     3901 tctgtctctc tcgtcggtta caccaatgcg ggcaagtcga cgctgttcaa cgcgctgacc
     3961 aaggcgggcg cctacgcggc ggaccagctc ttcgccaccc tcgacaccac ctcgcgcagg
     4021 ttgtacgtcg gcggcgaggg ggcaggtgcg agcgttgtgc tgtcggacac cgtcggcttc
     4081 atccgcgacc ttccgcacgc cctcgtggcg gccttccagg ccactctcga ggaaacggcg
     4141 caggccgacc tgctgttgca cgtcgtcgac tcggcgagcg aggaccgcga tgcgcagatc
     4201 ggggcggtga accaggttct cgccgagatc ggcgcggccg acgtgccgca gatcctggtg
     4261 tggaacaaga tcgacctcac ccgcgccgct gcggcggtcg agagggggga ctgtggtacc
     4321 atcaggcgca tttttttgag cgcccgaacc ggggaaggcc tcgatctgct tcgcgacgcg
     4381 cttgccgaag tagctcggca gacctttggt gacaacgccg accggcccgc cggtcgggat
     4441 gacgagcgac gacaagaaac gtgataacag gcattctcat gtcactgaac gacccacgct
     4501 ggggtggcca aggcggcggc gaccgcgaag acggcaatcg cggcgacgac aaccgcaacg
     4561 acgaccagcg cggcgatcgc aatcgcggtg gcaaccaggg gccgcccgac ctcgaggagg
     4621 tctggcacga cttcaaccag cgcctttccg gcatgttcgg cggcaaacgc cccgggcgtg
     4681 gtggcggctt cggcggcggc aatggtggcg gcaaccgccc cgagctcccc ggcttctcct
     4741 tcaagcagtt ccgtggcggc ttcggcgtcc ttgcggcgct ggtgctggtg gtctggctgg
     4801 cgagcggcct gtacacggtg gacgccaacc agcgcgccgt ggtgctgcgc ctcggcgagt
     4861 acgtcgccac caccgagccc ggcctgcgct ggcgcctgcc cgcacccttc gagacccacg
     4921 agatcgtcga cctcacgggc gtgcgcacgg tcgaggtggg ctatcgcggt tccgagcgca
     4981 acaaggtgct gcgcgaatcg ctgatgctca ccgacgacga gaacatcatc aatatccagt
     5041 tcgccgttca gtacgtcctc aacagcccgg agaactacat cttcaacaac cgtttccccg
     5101 acgaggccgt ggcgcaggcc gcggagaccg cgatgcgcga aatcgtcggc aagagccgca
     5161 tggacttcgt gctttacgag ggccgcgagg agatcgcgac caccgcgcac gagctcatgc
     5221 agcgcatcct cgaccgctac gagaccggca tccaggtcag ccgcgtcacc atgcagaacg
     5281 cccagccgcc cgagcaggtc caggctgcgt tcgacgacgc ggtcaaggcc gggcaggacc
     5341 gcgagcgcca gaagaacgag ggcgaggcct acgccaacga cgtcgtgccg cgggcccgcg
     5401 gtaccgcttc gcgcctggtc gaggaagcca acgcctaccg cgagcgcgtg gtcgccaatg
     5461 ccgagggcga ggcgagccgc ttcagccagg tgttcgcaga atacaaccgc gcgccggagg
     5521 tcacccgcga gcgcctgtat ctcgacacca tgcagcaggt catgtccagc acctccaagg
     5581 tgatggtcga cgcgaagggc aacggcaacc tgctgatgct gccgctcgac aagctgatgc
     5641 agcagacggg cgccaagccc gccgcggcgg cgcctcccga ctcgcagtcg gccgcgggcc
     5701 tcgcggccaa cgctccggtg ccgtccttcg atccgcgcaa ccgggatgga atgttcagtc
     5761 gtgagcgggg agtacgttga tgcgtgacaa gatgtccctg atcggcggca cgctgctgct
     5821 gctcgtcgtg atcgcctcga tgtcgctgtt caccgtcgac cagcgccagt atgcgatcgt
     5881 cttccagctc ggcgaggtca aggaggtcat ctccgagccc ggcctcaacg ccaagctgcc
     5941 tttcatccag aacgtgcgct acttcgacaa gcgcatcctg acgatggaca cgcccgaacc
     6001 cgagcgtttc atcacctccg agaagaagaa cgtgctggtc gaccacttcg tcaagtggcg
     6061 catcgtcgac ccgcgcctgt actacgagtc ggtggccggc gacgaggcgc gtgcgcgcac
     6121 ccgcctgacg cagacggtca acgccggtct gcgcgaggag ttcggccgcc gtaccgtgca
     6181 cgacgtcgtc tccggcgagc gcgaccgcat catggagcag atgcgcgagc gcgcggaccg
     6241 cgacgcccgc accatcggcg tgcagatcgt cgatgtgcgc ctgaagcggg tcgacctgcc
     6301 caacgaggtc tcggagtcgg tctatcgtcg catggaggcc gagcgcaagc gcgtcgccaa
     6361 cgagttgcgc tcgctgggtg ccgcggaggc cgaacgcatc cgcgccgacg ccgaccgtca
     6421 gcgcgaggtg atcatcgccg aggcttaccg cagtgcgcag gaggtcaagg gtgcgggcga
     6481 cgcgaaggcg accgccatct acgccgaagc cttcggcaag gaccgcgagt tctactcctt
     6541 ctaccgcagc ctcgaggcct accgcgcgag tttctcgggc aaggacgacg tgctggtggt
     6601 cgatccgagc tcggatttct tccgtttcat gaaggatgcc ggaggcgcgc cgcgcaactg
     6661 acgtcttcat ggcaagctcg ctcctcacgg ccttcgcgct gatgctgatc gtcgaaggcc
     6721 tgttgccctt catcgcgccc gccgcatggc gcgagacctt ccttcgcctc gccagcatgg
     6781 ccgacggcca gatccgcttc atcggtctga gttccatgct ggcggggctc gcaatgcttt
     6841 tcatcgttct ctgacaccgc catgcgctgg gtactgcccg accacatcca ggacgccctg
     6901 ccgtccgacg cccaccggct ggagagcctg cgccgccgcc tgctcgacgc cttccggctg
     6961 cgcggctatc agctcgtgat gccgccgctg ctcgaatacc tcgactcgct caccaccggc
     7021 gccggcaagg acctcgagct gcgcaccttc aagctggtcg accagctctc cggccgcagc
     7081 atgggcgtgc gtgccgacat gactccgcag gtcacccgca tcgacgccca ccttctcaac
     7141 cgccgcggcg tgtcgcgctt gtgttattgc ggcagcgtgc tgcacaccct gccgtccacg
     7201 ctcaccggca cgcgcgagcc gctgcagctc ggcgctgagc tctacggcca tgccggcatc
     7261 gaggccgacg tcgagatcct gcgcctgctc gccgaggtgc tgcgcctcgc cgaagtgcct
     7321 gcctcgcgca tcgacatcgg ccatgtcgga ctcttccatg cgctcgccgc gcgcgcaggg
     7381 ctggtgccgg gccgcgagga agagctcttc gacctgctgc aggcgaagga cgtgcccgag
     7441 ctgcgccgca tgctcgccga cgtctccgag ccggcgcgca gcgcgctgct ggcactgccc
     7501 gaactctacg gcggccccga ggtgctcgca gaggccgccg cacgactgcc gcaggatgcc
     7561 gagatcgccg gcccgcttgc cgaactgcgc caactcgccg acgcgctggg cgacctcccg
     7621 gtgagcttcg atctcgccga cctgcgcggc taccactatc acagcggcgt cgtgttcgcc
     7681 gcctacggcg ccgaatcgcc cgccgcgctc gcgctcggcg gccgctacga ccgcgtcggc
     7741 caggcgttcg ggcgcgcacg gccggccacc ggcttcagcc tcgacctgcg cgaactcgcc
     7801 tggcacctgc ccaccccggc ggcgccggcg ggcgcggtgc tggcgccgcc ggacgacgac
     7861 gcggggctga cctccgaggt ctccgcgctg cgcgcacgtg gcgagatcgt gatggtggcg
     7921 ctgccgggac acgaaggaac ttggaacgaa gccggctgcg accggcaact ggtgaagcgg
     7981 ggcgatcgct gggcggtcgt gccgttacag ggagagtaag gaaatggcaa aaaacgtcgt
     8041 cgtcgtcggc acccagtggg gtgatgaagg caagggcaag atcgtcgact ggctcaccga
     8101 ccacgccaag ggcgtggtgc ggttccaggg tggccacaac gccggtcaca cgctggtcgt
     8161 cggcaagacc gaatacaagc tgaacctggt gccctcgggc atcgtccgcg aaggcgtggc
     8221 ctgcttcatc ggcaacggtg tggtgctcga tgcgcaccac ctgttgtccg agatccgcac
     8281 gctggaagcc ggcggcatca aggtgcgcga ccgcctgcgc gtgagcccgg gctgtccgct
     8341 gatcctcggt taccacgccg cgctcgatcg tgcgcgcgag gccgccaagt ccgccggcga
     8401 caagatcggc accaccggca agggcatcgg ccccacctac gaggacaagg tcgcccgccg
     8461 tgcgctgcgc gtgtacgacc tcttcgaccg cgagcgcttc gccgccaagc tgaaggccaa
     8521 cctcgagtac cacaacttcg tgctcacccg acacctcggc gccgagccgg tcgatttcca
     8581 gaccgtgttc gaccaggcca tggccgacgc cgacgagctg ctgccgatgg tcgccgatgt
     8641 ctcggccgag ctctacgcga tcaacaagtc gggcggctcg ctgctgttcg aaggcgcgca
     8701 gggcaccctg ctcgacatcg accacggcac ctaccccttc gtcacctcga gcaactgcgt
     8761 ggccgggcag gccgcggcgg gctcgggcgt cggccccggc cgcctgcatt acgtgctcgg
     8821 catcacgaag gcctattgca cccgcgtcgg cggcgggccc ttcccgaccg aactcgacat
     8881 cgagaccaag ggcgtgcccg gcgagcagat gtcgaccaag ggccgcgagt tcggcaccgt
     8941 caccggccgc aagcgccgct gcggctggct cgatctcgcc gcgctcaagc gctcgatcat
     9001 catcaacggc gtgaccggcc tgtgcatcac caagctcgac gtgctcgacg gtctcgaaga
     9061 gctcaagctg tgcaccggct acatgctcga cggcaggcgc atcgacctgc tcccgatggg
     9121 ttcggaggag gtcacgcgct gcgagccgat ctacgagacc ctgaaaggct ggagcggcac
     9181 cacgtttggc gcgcagagct gggacgccct gccgcaggag gcgcgcacct acctgcaccg
     9241 catcgaggag atctgcgaga tcccgatcga cgtcatttcg accggtcccg agcgcgacga
     9301 aaccatcctc cgccgccatc cctttggcgc ctgagcatcc gccgcaccct gtgcggccgc
     9361 actccgcggc cgccgcgccc gccggggggc aatgcacccc ccgcgccatt ccagcacgac
     9421 gctcgatcgc gactcgagtc gttcctgcac ggggccaacc cgcgccgtcc gcgaatgttg
     9481 cgccatccgc agcgttcgcg aattccgcgc cgtttttcat aggggcgacg caagtcgcga
     9541 tcctcgcgtc cggcccgcat ggcgcacctt ccttcgcgcg acgatggcac aaacgcaaaa
     9601 aagccgctcc gaagagcggc tttcctgtga aactggtgcc cagaagagga ctcgaacctc
     9661 cacgcctcgc agcgctagta cctgaaacta gtgcgtctac caattccgcc atctgggcag
     9721 ggaggcgcat aatagcaaag gccttaactt ccgcaaaggt tttttttgca tcatcgggcg
     9781 catccttccg tcgccgagcg aaggggctgc ctttgactcg cgatgccggc acctcgatcc
     9841 cgcccagcat gtgttcccac tcgacgcccg ctcgcgactc ggatcgttcc tgcggacggc
     9901 ccgcagcagt gtccgtggat cccgcgccgt gcggcgattg tgcgcaggtc atcgtgggag
     9961 ccgccgcaag gacgcaagtc gcgacctgcg catgcagcct gcaccgcgcg tcctccagcg
    10021 cgccgc
//
LOCUS       DAOQNN010000075         9990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3199_length_9990_cov_9.123402, whole genome shotgun sequence.
ACCESSION   DAOQNN010000075 DAOQNN010000000
VERSION     DAOQNN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3199_length_9990_cov_9.123402"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /gene="peaB"
                     /locus_tag="PKV97_06145"
     CDS             <1..1189
                     /gene="peaB"
                     /locus_tag="PKV97_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase maturation
                     protein"
                     /protein_id="HNB05495.1"
                     /translation="EHGEDGRINPPAGEVTRFPLDTLVLNVNTGCNLSCTYCYKEDLS
                     APAKGLRMSLETATKAIEMMLAESPGKPKYTLVFFGGEPLSNMGLIRAVVDFAERRFA
                     EVGARVDFTLTTNATLLTAEIIDWLDAHRVGVTVSMDGPPAVHDRNRRSFGGEGTYDV
                     VRRKVDLLLARYRARPVGCRVTLTRGITGVVPIFEHLHHEVGFAEVGFGPVTSGDIQA
                     FNLTADELAEVFDGLKALGRRYLEAALRNRSLGFGNLHQLMTDLHEGNKKRLPCGAGV
                     GLLAVDGKGGLNLCHRFTGSALPTFGDVDAGIDRARLGAFIGRRLDRNGTGCATCRIR
                     NLCSGGCYHESYAKYGDAAAPSYHYCELLRDWIDFGIETYARIVTENPGFFAAHVSPR
                     RTA"
     gene            1186..1512
                     /gene="qhpC"
                     /locus_tag="PKV97_06150"
     CDS             1186..1512
                     /gene="qhpC"
                     /locus_tag="PKV97_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     gamma"
                     /protein_id="HNB05496.1"
                     /translation="MKHLKSLNKKAQRLDAAEGKPEAIEEVVAMQTVVGCTATTDPGW
                     EIDAFGGAASLCQPMEADLYGCADSCWWPAQVPDTMSHYPDWGDGKADATRDWRKLDG
                     IFEDKI"
     gene            1513..2658
                     /gene="peaD"
                     /locus_tag="PKV97_06155"
     CDS             1513..2658
                     /gene="peaD"
                     /locus_tag="PKV97_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009310 - amine catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     beta"
                     /protein_id="HNB05497.1"
                     /translation="MGAILMSLIPDLRALRAAGAIAAAALATACATPAQKSATSDAEY
                     LLTVTRPGLLHVIDMKTDRIARSCELPGKFGSGALVPTRDGRHAFVLGNLWEDVYGFE
                     LETCKLVFSARQSEGDVTVKTVQSLAVSADGSELYTVQNPVRRGADRFEVLPPRLAVY
                     RIADGLDAKPVRSFPVKRRITKIAATAGGEVILGGADVEAIDVRSGAVRTVSALASWD
                     RGPRWLTPDAFAMHSQGEHLNEYVMPYVTAKFADDRQDMASAEWWWGMSRVDLATGKA
                     EAREIFPFEFVIFSLVADPRDRNILYGAFNTLSRHDIEKRETLKVVELDHTYYSLQLS
                     RDASRVYVGGAGSTISIHDSGTLEKIGTIQLPGDMSTADVRVAWRRP"
     gene            2794..3828
                     /locus_tag="PKV97_06160"
     CDS             2794..3828
                     /locus_tag="PKV97_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HNB05498.1"
                     /translation="MTRTPKVCFDRVLPRDLMRFQTTMPDRPGQVRGLSPIGKTWING
                     STLHVRFMGGSAQQKKKVQEQAAWWTAHANLQFVFDDAPAAEIRITFDENDGAWSYIG
                     TDAKSIPVNEPTMNLGFLDGGTAAHEFGHAIGLAHEHQNPAGGIQWNEQAVIRAMAGP
                     PNHWSETQTRHNILRKYAANQVNGTDFDPKSVMLYFFPAEWTLNGVATSRNEVLSELD
                     KQFIAGAKMYPRAKPGVTGEVTELKVNARRRTAASIGKFGEEDVFRFRVTQPGRYVVD
                     TRGPTDVYMKLFGPDSPTALIAEDDDSGLGSNARIATDLVEGEYFVQVRHWNRASGVG
                     DYTVAVRTAR"
     gene            complement(3870..4346)
                     /locus_tag="PKV97_06165"
     CDS             complement(3870..4346)
                     /locus_tag="PKV97_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB05499.1"
                     /translation="MGGGNDNLAVPTSTEAITFGMLPELIGYRLRIAQIGIFRDFCTS
                     VDEAEITPTLFGVLVLIDANPGMKQTQLADAIQLDRSSVVSVLDKLEARGLVERVRAP
                     DNRRANALHLTAAGRHLLHSLIPQVRAHEERVLQDLDAAERAQLRKLLGRLVLAAG"
     gene            4790..5605
                     /locus_tag="PKV97_06170"
     CDS             4790..5605
                     /locus_tag="PKV97_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis F family
                     protein"
                     /protein_id="HNB05500.1"
                     /translation="MNQDWIPVGALGDAFAADNNCLPPVDDLADTNLTLHFENGWVIR
                     HHFITDRELSWEILEGDAALMLDTESYVATRPRAGIYFVDFVKRSERATSVSLVLMIE
                     SGVFLAAIGQLPSREEASLSLLDRIDAKLELTAVRADFIRGTINSERSPDRSFPAPTR
                     DLVGKRIEYRYSATELYEHLYLNERFYSWHCLEGSEVGLCDTDACHFFKLSEGLYFFV
                     WREKVVPTLGVICIDLVAMKTTGKIFGYESNECSATRNFPVGAYVRNVHVAAR"
     gene            5689..7092
                     /locus_tag="PKV97_06175"
     CDS             5689..7092
                     /locus_tag="PKV97_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNB05501.1"
                     /translation="MNEMPVVDARERAHLPRVLGLHTLVALIVGIAVSQVGLVGVLQG
                     IGLIGGESLPIIVGAFVTAVILAITYAASFSELSLMMPSSGGLSRYTEIAMGHFPALL
                     ATFSGYVVVNMFGLSAELLLFDNIVREVFDLDIPHSMVALMLLGALAVLNIRGTDVFA
                     VLQNSSTAIKVAIMIASGAAVIWVQPGFWATAHQKGVEAGAFPGDIFPTGVALFFWCF
                     VAAEFVCPMMKETKAPERNIPRSMVVGIIMLAALYGCYAFGAVHFLSRETLVDSPFPH
                     LEYAKAVFGRAGSMVLMVFATAATIGLINGVLAGVSRMLYGMAHDGQAFRFLGKLHPT
                     YGTPWVGIIFMAFICGAPLVLLGDEPATIMTLVVSASTSWLLAYIVAHLDVIILRIRY
                     PMAARPFRTPFFPVLPMLGIGGMGYVIIHNPVSVLIMTGLVLGVVGLISAIWVRLVMK
                     RPLLAPKPWSGRVGAQE"
     gene            complement(7111..7824)
                     /locus_tag="PKV97_06180"
     CDS             complement(7111..7824)
                     /locus_tag="PKV97_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB05502.1"
                     /translation="MIQSEREEEKTDEKSLSQFADAFAACAGKIIPNTGWVTYVVGPG
                     LRIKHFLSCETNGAERADYEFNFAHLDPLSPMHCIARQRFVACLHDELSTHSPDHLAY
                     RQYFMQPHRIVDALEIILRSESGICVGISLIRHHHAPEFSALEKRQADALRGLGDFIL
                     SRLLPTRLAGVDFICERFPAVTPREAALMQLVLMGLSNKQICRELSISLPTVKSHLLN
                     IFRKANVHSRTELIARILC"
     gene            8246..9175
                     /locus_tag="PKV97_06185"
     CDS             8246..9175
                     /locus_tag="PKV97_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB05503.1"
                     /translation="MGLNAWHVVYEQLTPGRFEGGVEELWIDDGLEILRETGNRSVWT
                     AGCNRLGMVSLGIPVAGGGAGMYCGVPIQDGAVSWLPGGGDFEIFSRERMDIVSATVS
                     ESLLCAFAAIDSPQTMEASWRRPFLRQQPRHAALLRRALIEIVAAVRRQPGLLEVSAS
                     RAAMRDCVLSLMLDTLSSASARPTPDLRPSVKAWIVRKVREQALASPSEPLHVADVCR
                     SLAVSRRSLQYAFEDLAGMGAVEFLRNVRLNAVRRELRVPVACPDEPIAAIAARWGFW
                     HMPRFAAYYRALFGERPSETRRRQGLSTLHGLG"
     gene            9341..>9990
                     /locus_tag="PKV97_06190"
     CDS             9341..>9990
                     /locus_tag="PKV97_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB05504.1"
                     /translation="MEDKTCAAPAAGAGGSARRRFLKFASAAAAASALPAGAVVAAPA
                     QPGYDVVVVGGGFAGVTAARELAHRGARVVLLEARNRLGGRTFHANFGERKVELGGTW
                     IHYSQPHVWAEVMRYGMEIAETPGVAHPDRVLWMSGGKVLEIPVMENWALLEDALKRF
                     HAEAGEVFARPFMAGLSAAGRKLDHLSIADRMAAMDMSPAQRDLMNAMMATNCHGP"
BASE COUNT         1666 a         3114 c         3327 g         1883 t
ORIGIN      
        1 cgagcacggc gaggacggcc gcatcaatcc gcccgcgggc gaggtgacgc gctttccgct
       61 cgacaccctg gtgctcaacg tcaatacggg ctgcaacctg agctgcacct attgctacaa
      121 ggaagatctc agcgcgccgg ccaagggcct gcgcatgagc ctggagacgg cgacgaaggc
      181 gatcgagatg atgctcgccg agtcgcccgg caagccgaaa tacaccctcg tcttcttcgg
      241 cggcgagccc ctgtcgaaca tgggcctgat ccgcgcggtg gtcgacttcg ccgagcgccg
      301 cttcgccgag gtcggcgcgc gcgtggattt caccctcacc accaacgcca ccctgctcac
      361 cgccgagatc atcgactggc tggacgccca ccgcgtgggg gtcaccgtgt cgatggacgg
      421 gccgcccgcg gtgcatgacc gcaaccggcg cagcttcggc ggcgagggta cctacgacgt
      481 ggtgcggcgc aaggtcgacc tgctgctcgc gcgctaccgt gcgcgcccgg tcggctgccg
      541 ggtcacgctc acccgcggca tcaccggggt ggtgccgatc ttcgagcacc tgcaccacga
      601 ggtcggcttc gccgaggtcg gcttcggccc ggtgacctcg ggcgacatcc aggccttcaa
      661 cctgaccgcc gacgagctcg ccgaggtgtt cgacggcctc aaggcgctcg gtcggcgcta
      721 tctcgaagcg gcgctgcgca accgcagcct cggcttcggc aatctgcacc agctcatgac
      781 cgacctccac gagggcaaca agaagcggct gccctgcggc gccggggtcg ggctgctcgc
      841 ggtcgatggc aagggcgggc tcaacctttg ccatcgcttc accggctccg cgctgcccac
      901 cttcggcgac gtcgatgccg gcatcgaccg cgcccgcctg ggcgccttca tcggccgccg
      961 cctggaccgc aacggcaccg gctgcgcgac ctgccgcatc cgcaacctct gctccggcgg
     1021 ctgctaccac gagagctacg ccaagtacgg cgatgccgcc gcgccttcct atcactactg
     1081 cgaactgctg cgcgactgga tcgacttcgg catcgagacc tacgcgcgca tcgtcaccga
     1141 gaaccccggc tttttcgccg cacacgtttc cccgcggagg accgcatgaa gcacctgaag
     1201 tcgctcaaca agaaggcgca gcgcctggac gccgcggagg gcaagcccga ggcgatcgag
     1261 gaggtcgtcg ccatgcagac ggtggtcggc tgcaccgcga ccaccgaccc gggctgggag
     1321 atcgacgcct tcggcggcgc cgcctcgctg tgccagccga tggaggccga cctctacggt
     1381 tgcgccgact cgtgctggtg gccggcgcag gtgcccgaca ccatgagcca ttaccccgac
     1441 tggggcgacg gcaaggccga tgcgaccagg gactggcgca agctggacgg catcttcgag
     1501 gacaagatct gaatgggagc gatcctcatg agcctcatcc ccgacctgcg tgcgctgcgg
     1561 gcagccggcg cgatcgccgc cgcggcgctg gcgaccgcgt gcgcgacccc ggcgcagaaa
     1621 tccgcgacga gcgacgcgga gtatctgctc accgtcaccc gccccggcct gctccacgtc
     1681 atcgacatga agaccgaccg catcgcgcgc agctgcgagc tgcccggcaa gttcggctcg
     1741 ggcgcgctgg tgccgacgcg cgacggccgt cacgccttcg tgctcggcaa cctgtgggag
     1801 gacgtgtacg gcttcgagct cgagacctgc aagctggtgt tctccgcccg ccagtcggaa
     1861 ggcgacgtca ccgtcaagac ggtgcagtcg ctcgcggtga gcgccgacgg cagcgagctg
     1921 tacaccgtgc agaacccggt gcgccgcggt gccgaccgct tcgaggtgct gccgccgcgc
     1981 ctcgcggtat atcgcatcgc cgacgggctg gacgccaagc cggtgcgcag cttcccggtc
     2041 aagcgccgca tcaccaagat cgccgccacc gccggcggcg aggtgatcct cggcggtgcc
     2101 gacgtggagg cgatcgacgt gcgcagcggc gcggtgcgca ccgtgtcggc gctggcgagc
     2161 tgggaccgcg gtccgcgctg gctgacgccg gacgcgttcg cgatgcactc gcagggcgag
     2221 catctgaacg agtacgtgat gccctacgtg accgcgaagt tcgccgacga ccgccaggac
     2281 atggcgagcg ccgaatggtg gtggggcatg agccgggtgg acctcgcgac cggcaaggcc
     2341 gaggcgcgcg agatcttccc cttcgagttc gtgatcttct cgcttgtcgc cgatccgcgc
     2401 gatcgcaaca tcctctacgg cgccttcaat acgctctcca ggcacgacat cgaaaagcgc
     2461 gagacgctga aggtggtgga gctcgaccac acctactaca gcctgcaact gtcgcgcgac
     2521 gcctcgcgtg tttacgtcgg cggggcgggc agcacgatca gcatccacga cagcggcacg
     2581 ctggagaaga tcggcacgat ccagctcccg ggcgacatgt cgaccgccga cgtgcgcgtg
     2641 gcctggcggc gcccgtagtc gcggcgcgtg tttccctggc gcctgacggg ggcccgtact
     2701 atatgttcag caggagccgg ccgctgccgc gaccggattc ggcgaacgcc gcttgctgcg
     2761 gcgcgtcgcg gccacgtcag ggagggatcc gccatgaccc gaacgcccaa ggtgtgcttc
     2821 gaccgggtgc tgccccggga cctgatgcgc ttccagacca ccatgcccga ccggccgggc
     2881 caggtgcgcg gactatcgcc gatcggcaag acctggatca acggctccac cctgcatgtg
     2941 cgtttcatgg gcggaagcgc gcagcagaag aagaaggtgc aggagcaggc cgcgtggtgg
     3001 acggcgcacg ccaacctgca gttcgtgttc gacgacgccc ccgcggccga gatccgcatc
     3061 accttcgacg agaacgacgg agcctggagc tacatcggca ccgacgcgaa gagcatcccg
     3121 gtcaacgagc cgacgatgaa cctcggcttc ctcgacggcg gtacggcggc gcacgagttc
     3181 ggccatgcga tcgggctcgc gcacgagcac cagaaccccg ccggcggcat ccagtggaac
     3241 gagcaggcgg tgatccgcgc catggcgggg ccgcccaatc actggagcga gacccagacc
     3301 cggcacaaca tcctgcgcaa gtacgccgca aatcaggtca acggcaccga cttcgacccc
     3361 aagtcggtga tgctgtactt cttccctgcg gagtggaccc tcaacggcgt ggcgacctcg
     3421 cgcaacgagg tgctgtcgga gctcgacaag cagttcatcg ccggcgcgaa gatgtatccg
     3481 cgcgccaagc ccggcgtaac gggcgaggtg accgagctga aggtcaacgc acgccggcgc
     3541 accgccgcca gcatcggcaa gttcggcgag gaggacgtgt tccgcttccg cgtgacgcag
     3601 cccgggcgct acgtggtcga cacgcgcggt ccgaccgacg tgtacatgaa actcttcggc
     3661 cccgattcac cgaccgcgct gatcgcggag gacgacgact ccggcctggg cagcaacgcg
     3721 cgcatcgcga cggatctggt cgaaggcgag tatttcgtgc aggtgcgtca ctggaatcgc
     3781 gccagcggcg tcggggatta caccgtggcg gtgcgcacgg cgcgctgagt ctgctgcctg
     3841 cgccgagtcc gcactcctcg cccgtcgcct cagccggccg cgagcaccag gcggccgagc
     3901 agtttcctca actgcgcgcg ttcggcagcg tcgaggtcct ggagcacgcg ttcctcgtgt
     3961 gcccgcacct gcggaatcag gctgtgcagc aggtgccggc cggcagcggt cagatgcagg
     4021 gcgttggcgc gccgattgtc gggtgcgcgc acgcgttcga ccaggccgcg cgcctccagc
     4081 ttgtccagca ccgacaccac gctcgagcgg tcgagctgga tcgcgtcggc gagctgggtc
     4141 tgcttcatgc ccggattggc gtcgatcagc accagcacgc cgaacagcgt gggggtgatc
     4201 tcggcctcgt ccacgctcgt gcagaaatcg cgaaagatgc cgatttgcgc gatgcgcagg
     4261 cggtagccga tcagctcggg gagcatcccg aaggtgatgg cctccgtaga ggtcggcaca
     4321 gccaggttgt cgttgccgcc acccatcggt tgctcgagtc cagttgaata ggtcccggat
     4381 tgtagcaggc cgatccccac aggcggacgc caacggccgc agggcaatga cgtgaccgcc
     4441 ctctggcggt gcgcttcccg cgggtgccgc atgaaccagg gcactcggca tgtgccttca
     4501 ctgccgcgtc catccaacta cgcgctccaa ctcctccgtc gtgcataccc gattgccagg
     4561 gggggcgatc gagaaccatg acgtgcgcgc acgtgggtca ttgcctggcc tcggaccccg
     4621 catgcgggct ttgtgtatcg gccggccact cgccgggtac ccggcctcgc ttccccgttc
     4681 ggctgcgtcg gtggaggggc atcatacttt cggttgatgg acatgaattc gcggcgttgg
     4741 ataatcagat ccacgcctgc tgatcagccg ctatctggag aaacagaaaa tgaatcagga
     4801 ttggattcct gtcggtgcgc ttggtgatgc ctttgccgcc gataacaact gccttccgcc
     4861 ggtcgatgat ctcgcggata cgaatttgac gctccatttt gagaacgggt gggtcattcg
     4921 tcaccacttc atcacggatc gcgagttgtc gtgggagatt ctggaaggcg acgcagcgct
     4981 catgttggat acggagagct acgtcgcgac gcggccgcgt gccggcatct attttgttga
     5041 cttcgtgaag cgttcggagc gcgcgaccag cgtcagtctg gtgttgatga tcgagagtgg
     5101 cgtctttctt gcggctatcg gtcaactgcc aagcagggaa gaagcgtcgc tatcattgct
     5161 tgatcggatc gacgctaaac tcgagttgac agcagtgagg gcggatttca ttcgtggaac
     5221 gataaattca gagcgctctc ccgatcggtc gttcccggct ccgacccggg atcttgtggg
     5281 taagcgaatt gaataccgtt acagtgcgac ggaactctac gagcatctgt acctgaatga
     5341 aaggttctat tcatggcatt gcctggaggg ctcggaggtg gggctttgcg ataccgatgc
     5401 atgccatttc ttcaagctga gcgaggggct ctacttcttt gtctggaggg agaaggtcgt
     5461 gcccacgcta ggcgtcatct gcatcgatct tgttgccatg aaaacgactg gaaaaatttt
     5521 tggctacgag tcgaatgaat gcagtgcaac gcgcaacttc ccggtcggag cctacgtgcg
     5581 aaacgtgcat gtcgcggctc ggtaagtgga actgcatggt tcattatcgg gcgcggccgc
     5641 gatcggcagc aatcggttcg gcaggcatga cataacgagg aaagcgtgat gaacgagatg
     5701 ccggttgtcg atgcgagaga aagggcgcac ttgcccagag tgttggggtt gcacaccctg
     5761 gtcgcgctga tcgtcggcat agccgtgtcc caagtcggcc ttgtcggcgt tctgcagggg
     5821 atcggcctga tcggaggcga atcgctcccg atcatcgtcg gcgccttcgt caccgccgtc
     5881 atcctggcga tcacctacgc ggcctccttc tccgagcttt cgctcatgat gccgtcctcc
     5941 ggcggattga gcagatacac ggagatcgcc atgggccact ttcccgcgtt gctcgccacg
     6001 ttttccggct acgtggtggt gaatatgttc ggcttgtccg cggagctgct tctgttcgac
     6061 aacatcgttc gcgaggtctt cgacctggac attcctcata gcatggttgc gttgatgctc
     6121 ctcggtgcct tggccgttct gaacatacgg ggtaccgatg tcttcgccgt cctgcagaac
     6181 tcgtcgacgg cgatcaaggt cgccatcatg attgcaagtg gagccgcggt gatctgggtt
     6241 cagcccgggt tctgggcgac ggctcatcaa aagggtgtcg aagccggcgc atttccggga
     6301 gacatcttcc cgactggcgt cgcgctcttc ttctggtgtt tcgttgctgc ggagttcgtt
     6361 tgtccgatga tgaaggaaac gaaggctccg gagcggaaca ttccgcgatc gatggtcgtc
     6421 ggcatcatca tgctcgcggc gctctacggg tgctacgcgt tcggtgctgt gcatttcctg
     6481 agcagggaga cgcttgtcga tagtccattt ccgcatctgg agtatgcgaa ggcagtgttc
     6541 gggcgcgccg gttctatggt cctgatggtt ttcgcaactg cggcgactat cggcctgatc
     6601 aatggtgttc tggccggcgt ctcgcgaatg ctctacggaa tggcgcatga cggccaagcg
     6661 tttcggttcc tggggaagct gcatccgacg tatggcactc cttgggtcgg cataatcttc
     6721 atggccttca tttgtggagc gccccttgtc ctgctgggag acgagccggc cactatcatg
     6781 actctggttg tgtcggcttc gacgtcatgg ctcttggcat atatcgtcgc acatctggat
     6841 gtgatcattc tccgcatccg atacccgatg gcagccaggc cgttccgcac accattcttt
     6901 cccgtgctgc cgatgcttgg tattggcggg atgggttacg tcatcattca caacccggtt
     6961 agcgttctga tcatgactgg tcttgttctc ggggttgtcg gtctgataag cgcgatatgg
     7021 gttcgactgg tgatgaagcg accgcttctg gctcccaagc cgtggtcggg gcgcgttggc
     7081 gcgcaggagt aggaatcacc gtcgggctgg ctagcacaga attctcgcaa tcagttcggt
     7141 tcgactatgg acgttagcct tgcgaaagat gttcaataga tggctcttta ccgttggcag
     7201 ggatattgaa agctcacggc atatctgctt gttgctcagc cccatcaata caagttgcat
     7261 cagggcagcc tctcgtggag tgactgccgg gaatcgttca caaatgaagt cgaccccggc
     7321 gagccgggtg ggcaatagtc ttgagaggat gaaatcgccg agacctcgca aagcgtcggc
     7381 ttgcctcttt tcgagcgctg agaactctgg agcgtgatgg tgccggatca atgaaatgcc
     7441 cacgcaaatg ccggattcgg aacgcaggat gatctctagg gcatcgacga tgcgatgcgg
     7501 ttgcatgaag tactgtctgt atgccagatg gtcgggagaa tgtgtcgaaa gctcatcgtg
     7561 caggcaggcg acgaagcgct gccgagcaat gcagtgcatg ggagagagtg ggtcgaggtg
     7621 ggcgaagttg aactcgtaat ctgcgcgctc ggcgccgttc gtttcgcacg agagaaaatg
     7681 ctttattctt agccctgggc cgacaacata ggttacccat ccagtatttg gaatgatttt
     7741 tccggcacag gctgcgaagg cgtcggcgaa ttgagagagc gacttttcgt cggtcttttc
     7801 ttcttcccgt tcgctttgga tcatggtgag ttccattaag atgcgtcaaa cagacggccg
     7861 cggagctcga gttgtggaac tgcggcgctt gtctgaattc tgactagacg tcaattcgct
     7921 tgctagtttc catgatgtgg aggataccgc ggaagccgtc tggatagcgt gaccggattc
     7981 attgctgcca ccggtttcgc gcaatcggtt cattgaggta cgctgcaaga caaatgggga
     8041 aatcgtcgtc ggggacgccg ccgggtctgg gcttgcacgg ggaacgaggc ccctccgatc
     8101 caccgtgtcg tgcccacttc ggatgtgtct gcgcgtgtct gtcagttcct cggtacaacg
     8161 ttgctttact gatgtgtccg ttggggccct tgcgctgcct gacggccgag cgttgacgcg
     8221 cgtggacgat gtatgcgaat tcgggatggg gctgaatgct tggcacgtgg tgtatgagca
     8281 actcacaccg ggccgcttcg agggcggcgt ggaggagctg tggatcgatg acggcctcga
     8341 gatcctgcgg gagacgggca accgctcggt gtggactgca ggctgcaatc ggctgggcat
     8401 ggtgtcgctc ggcattccgg tggcaggcgg cggcgccggc atgtactgcg gcgttccgat
     8461 ccaggacggg gcggtgtcgt ggcttccggg cggcggagat ttcgagatct tctcccgtga
     8521 gcgcatggat atcgtctcgg cgacggtgtc cgaatccttg ttgtgcgcgt ttgccgccat
     8581 cgactcgccg cagactatgg aggcgtcgtg gcgacgcccg ttcctccggc agcagccgag
     8641 gcatgctgcg ctgctgcgcc gcgcgctgat cgagatcgtc gccgctgtcc gccgccagcc
     8701 cggcttgctg gaggtttcgg cgagcagggc ggcgatgcgc gactgtgtcc tgtccctgat
     8761 gctcgatacg ctgagctcgg cctcggcgcg cccgaccccg gacctgcgtc cgagcgtgaa
     8821 ggcctggatc gtgcgcaagg tgcgcgagca ggcgctcgcg agcccgagcg agccgctcca
     8881 tgtggccgac gtctgccgca gccttgcggt gtcgcggcgc tcgctgcagt acgcgttcga
     8941 ggatctcgcc gggatggggg cggtggagtt cctgcgcaat gtgcgtctga acgcggtgcg
     9001 gcgggaactt cgtgttccgg tcgcttgccc tgacgaaccc atcgccgcga tcgcggcgcg
     9061 ctggggcttc tggcacatgc cgcggttcgc ggcgtattac cgggccctgt ttggcgaacg
     9121 gccctcggag acgcgtcgac gccaaggcct gagtacgctg cacggcctcg ggtgagcgtt
     9181 tgctgcgctg cgtcggcatt tccgtgccga ttgcggatac cgtcctgttc atcggtcttc
     9241 ttaatctcca catcacgact cggccaggtg gtgcgagcgc gacacgccgc gggccctcac
     9301 ttccgagcac ggactgcggc gacggaggag atcggagatc atggaagaca agacctgcgc
     9361 ggcgccggcc gcgggtgccg gtggcagtgc gcggcggcga ttcctcaagt ttgcgtcggc
     9421 ggccgcggcc gcttcggcgt tgccggcggg tgccgtcgtt gcggcgcccg cgcagcccgg
     9481 ctacgacgtg gtggtggtgg gcggcggctt cgcgggagtc actgccgcgc gcgaactcgc
     9541 ccaccgcggc gcccgggtgg tgctgctcga ggcgcgtaat cgtctcggcg ggcgcacctt
     9601 ccatgcgaac ttcggcgagc gcaaggtgga actgggcggc acctggatcc actacagcca
     9661 gccccacgtg tgggccgagg tgatgcgcta cggcatggag atcgccgaaa cgccaggcgt
     9721 cgcccatcct gaccgtgtgc tgtggatgtc cgggggaaag gtgctggaga tcccggtgat
     9781 ggagaactgg gcgctgctcg aggacgcgct gaagcgcttc catgccgagg cgggcgaggt
     9841 cttcgcgcga ccgttcatgg cgggcctgtc ggccgccggg cgcaagctcg atcacctgtc
     9901 catcgccgac cgcatggcgg caatggacat gagtccggcg cagcgcgacc tcatgaacgc
     9961 gatgatggcc accaactgtc acggcccgat
//
LOCUS       DAOQNN010000076         9987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3202_length_9987_cov_10.535340, whole genome shotgun sequence.
ACCESSION   DAOQNN010000076 DAOQNN010000000
VERSION     DAOQNN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9987
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3202_length_9987_cov_10.535340"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /gene="waaA"
                     /locus_tag="PKV97_06195"
     CDS             complement(<1..1161)
                     /gene="waaA"
                     /locus_tag="PKV97_06195"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HNB05505.1"
                     /translation="MLTRLPYTLLWILALPLVLLRLAWRARHQPAYLRHVGERFGRYR
                     TRAPVGVIWVHAVSVGETRAAEPLVRALLAEWPEHSVLLTHMTPTGRDTSKTLFRDEP
                     RVLRAYLPYDLGCFAHAFLRHFRPLFGVVMETELWPNLLAACRRRRIPVMLANARLSE
                     RSARRYARLPALTALTLKALAAIGAQTAADAARLAQLGARRVTVTGNLKFDIAPPEAM
                     LALGRSLRERIGARPVLLAASTREGEEALLLDAFARLAPPAALLLLVPRHPQRFDEVA
                     ALARARGLALQRRSADEAVRADTRVLLGDSMGEMFAYYAAADVALIGGSWLAFGGQNL
                     IEACAVGTAVVIGPHTFNFQAVAEVAERAGAAVRAQDAEAGMREALALVADPE"
     gene            1305..2312
                     /locus_tag="PKV97_06200"
     CDS             1305..2312
                     /locus_tag="PKV97_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNB05506.1"
                     /translation="MKILVTGAAGFIGMHTTLRLLARGDEVVGLDNLNDYYDPTLKEA
                     RLARLQPHAGFRFVKMDVADRAGMEALFAAEKFDRVIHLAAQAGVRYSLQNPHAYVDS
                     NLVGFMNILEGCRHAKVQHLVYASSSSVYGGNTKMPFSESDSVDHPVSIYAATKKANE
                     LMAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRPIDVFNHGRMRRDF
                     TYVDDIVEGVIRTLDRIAEPDPAFDPMQPNPGTGSAPYRVFNIGNHDPVELMEFVAAI
                     EDALGTAAQKNFLPLQDGDVPATYADTAALNAWTGFAPATSVREGVGRFIAWYREYYR
                     V"
     gene            2402..3199
                     /locus_tag="PKV97_06205"
     CDS             2402..3199
                     /locus_tag="PKV97_06205"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNB05507.1"
                     /translation="MANYAIGDIQGCYAPLDRMLDRIAFDPAVDRLWIVGDLVNRGPE
                     SLRVLRFLRGLGDAASIVLGNHDLYLLMVAAGYKRRDSDDTLFQVLEADDRDELLEWL
                     SFQPLMRVHGDHVLVHAGLLPGWTVTRAQALAAEVGAVLAGPKSREFLLHLAGNRPDR
                     WSDGLKGWDRLRVIVNAMTRLRFCTAQGRMDLSAKGAPHKAPSGMLPWFRVPDRFSRT
                     HTIVCGHWSALGFYREPGLIALDSGCVWGGKLTALRIEDDAVFQVPG"
     gene            complement(3216..4049)
                     /locus_tag="PKV97_06210"
     CDS             complement(3216..4049)
                     /locus_tag="PKV97_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB05508.1"
                     /translation="MHAGAGTAPPASASGAATRAAAASEARAGGLLRATRWTRLALHL
                     LQGVLTIATIYPLTGRDTREALRQRWSQGLLRVLGMRLEHRGEAIAPGCMLVANHVSW
                     VDIFAINALAPAAFVSKSEVRAWPVIGWLAAKNDTIFLRRGSRGHARIINEETAALLD
                     AGCNVAIFPEGTTTDGATLLHFHAALLQPAIACGHPVQPLALQYRTPDDRFSRAPAYD
                     GELSLGECIANIIAAPRTIARITVAAPIATADGADRRTLAARTRGEIATAIGVALDAQ
                     T"
     gene            complement(4088..4855)
                     /locus_tag="PKV97_06215"
     CDS             complement(4088..4855)
                     /locus_tag="PKV97_06215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HNB05509.1"
                     /translation="MLQHLQPRQQTAARKTRNLHVGLASSETEILEAQKLRYRVFAEE
                     MGARLPTRSPGVDRDLYDPFCEHLIVRDEDAGRIVGTYRILSPSAARRVGGYYSENEF
                     DLTRLQHLRGQLVEIGRSCIDADYRSGAVIALLWSGLARYMQENGYEYLIGCASVSMA
                     DGGHAAASLYNRLREKHLAPLEYHVFPRCPLPLSSLRGDLDAEAPPLIKGYLRAGAWV
                     CGEPAWDPDFNTADLPILMPIGRVDGRYARHFMGRKD"
     gene            4967..5416
                     /locus_tag="PKV97_06220"
     CDS             4967..5416
                     /locus_tag="PKV97_06220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNB05510.1"
                     /translation="MQCTTPPDHDPDCVFCRIARGELPSSKVYEDADTLAIMDIQSVN
                     PGHMLVLVKPHRANIYALDDTLAGAAFRTAARVARVAKKVTGCEGVTLLQANEKAGAQ
                     TVFHFHLHVLPRWEGDGMALAWPVKNPPREALEAMAAKLREALAGQG"
     gene            5507..6442
                     /locus_tag="PKV97_06225"
     CDS             5507..6442
                     /locus_tag="PKV97_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNB05511.1"
                     /translation="MSILVCGSVAYDSIMVFHDRFRNHILPEQIHILNVSFLVPDLRR
                     EFGGCAGNIAYGLKLLGSDPLIVATVGEDAGPYRARLRALGLREDFVREVPGTFTAQA
                     FITTDLDDNQITAFHPGAMFHSHLNDVRTAAGATLGIVSPDGADGMLRHADGFAAAGV
                     PFVFDPGQALPILGSEALLKCLQQARYCTVNDYEARLMCEKTGRSEAELAREVEALVV
                     TLGAEGSRVHWRGEVFEVPAVRADAIVDPTGCGDAYRAGLLHGIAAGWDLRRSARLAA
                     VMGALKIAQRGGQNYAPSRADVGARYREAFGEDLW"
     gene            6436..6909
                     /locus_tag="PKV97_06230"
     CDS             6436..6909
                     /locus_tag="PKV97_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNB05512.1"
                     /translation="MVNRSGMKWRALAGAMIAALAVGGCAQGLGGGTYARTEARRAMT
                     VQFGTIEAVRAVQLEGTKTPIGSVAGAAIGGIAGNTVGGGRGQAVATVIGAVAGGLAG
                     SAVEEGATRQAGVEVTVRLDNDQFLAVVQADEGEGFRPGERVRVLRDAGTTRVSR"
     gene            complement(6996..8192)
                     /locus_tag="PKV97_06235"
     CDS             complement(6996..8192)
                     /locus_tag="PKV97_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HNB05513.1"
                     /translation="MMITRCPACQTVFRLRPEQLHARGGEVRCGHCFHPFNALEHTVE
                     VRDDGAAATAAPPPPAARPVYEARPDPLLDFDIPEVAPSPPIRAPLPEPGLDDYEDWL
                     APAHKPALRGTAMDDGQTGTAPAFDEGAAAAQPFPEVLRSARRGLPIDEDEGGTPARG
                     RDLAAPGPFAPTISYTPPPPFDDDPATAIPGLSTPVRESAQAAPEPASIPAHEDERPV
                     SPHEPDLARLDANYGRPRKETHPLVRALAMLLAVGLSALLAAQATYLYRMELARALPG
                     LRPVLSEACARFGCGVPYPRDAEHIAIEASDLQAEPGRPGYYLLQATLNNRADYPQQW
                     PHLELTLTDAGDNPISRRVLPSTDWLPPSQPHEAFMARSAIELRIPFAAPALAPTGYR
                     IYAFYP"
     gene            complement(8189..9091)
                     /gene="prmA"
                     /locus_tag="PKV97_06240"
     CDS             complement(8189..9091)
                     /gene="prmA"
                     /locus_tag="PKV97_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNB05514.1"
                     /translation="MWISVTLQAEADKAEALSDALMEAGALSVSIEDADAGTEAERPQ
                     FGEPGHLPTALWDHSRVIALFDGAADPAELGAMLAEAARAAGFDAVPPFSTETVAEQN
                     WVQLTQSQFDPIRITDRLWIVPSWHEAPDADAINIELDPGMAFGTGSHPTTRLCLEWL
                     CEVVTPGCSVLDYGCGSGILGIAAAKLGAGAVLGIDIDEKAVEAARDNAARNHAAVRL
                     QHSAVPVGDTFDLVVANILTNPLCVLAPAISARVAPGGRVALSGVLETQAAQVVEAWA
                     PHIALHVGAAHEGWIRLEGRRPGP"
     gene            complement(9098..>9987)
                     /locus_tag="PKV97_06245"
     CDS             complement(9098..>9987)
                     /locus_tag="PKV97_06245"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304287.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNB05515.1"
                     /translation="IKAAGGGGGRGMRVVHTEAALLNAVTTTRAEAQAAFGNPVVYME
                     KFLENPRHVEIQILADQHGNAVYLGERDCSMQRRHQKVIEEAPAPGIDRKTIAKVGER
                     CAEACRKIGYRGAGTFEFLYENGEFYFIEMNTRVQVEHPVTELITGIDIVQAQIRIAA
                     GEKLWFRQRDIEFRGHAIECRINAEDPFKFTPSPGRITNWHTPGGPGIRVDSHVYNGY
                     TVPPHYDSMIGKLIAYGDTREQAIRRMRIALSEMMVEGIKTNIPLHQTLMLDPRFNEG
                     CTSIHYLEHKLTHRNEVQD"
BASE COUNT         1421 a         3338 c         3717 g         1511 t
ORIGIN      
        1 ctccgggtcg gcgaccagcg ccagcgcctc gcgcatgccg gcctccgcat cctgcgcgcg
       61 caccgcggca ccggcgcgct cggcgacctc ggccaccgcc tggaaattga aggtgtgcgg
      121 gccgatcacc accgcggtgc cgaccgcgca ggcctcgatc aggttctgcc cgccgaaggc
      181 cagccagctg ccgccgatca gggcgacgtc ggcggcggcg tagtaggcga acatctcgcc
      241 catcgagtcg ccgagcagca cgcgcgtgtc cgcacgcacc gcttcatccg cactgcgccg
      301 ctgcagcgcg aggccgcgcg cgcgggcgag cgcggcgacc tcgtcgaagc gctgggggtg
      361 gcgcggcacc agcagcagca aggccgcggg cggcgccagg cgggcgaagg cgtcgagcag
      421 cagggcctcc tctccctcgc gggtgctcgc ggcgagcagc accgggcgcg cgccgatgcg
      481 ttcgcgcagg ctgcggccga gcgccagcat ggcctccggc ggggcgatgt cgaacttgag
      541 gttgccggtg accgtgactc ggcgcgcgcc gagttgggcg aggcgggcgg cgtcagcggc
      601 ggtctgcgcg ccgatcgccg cgagtgcctt cagggtgagc gcggtgagcg cgggcaggcg
      661 ggcgtagcga cgcgccgagc gctccgacag acgggcgttg gcaagcatca ccggaatgcg
      721 gcggcgccgg caggcggcga ggaggttggg ccacagctcg gtctccatca ccactccgaa
      781 cagcgggcgg aagtggcgca ggaaggcgtg ggcgaagcag ccgaggtcgt agggcaggta
      841 ggcgcgcagc acacgcggct cgtcgcggaa gagggttttc gaggtgtcgc gaccggtcgg
      901 cgtcatgtgg gtgagcagca ccgagtgctc cggccattcg gcgagcaggg cgcgcaccag
      961 cggttcggcg gcgcgggtct cgcccacgga gaccgcgtgc acccagatca cccccaccgg
     1021 cgcgcgcgtc cggtagcgcc cgaagcgctc gccgacgtgg cgcagatagg ccggctggtg
     1081 gcgcgcccgc caggccaggc gcagcagcac caggggcagg gcgaggatcc acagcaaggt
     1141 gtagggcagg cgggtcagca tcgggtggaa ggggaggacg accgccgcag tataagcaag
     1201 gacgccggcc gcggtcctgc gcgcgatgaa ggaaaccgtt cacactgtgc gctggcgtgg
     1261 gcatgcgata atttgtccaa tccatcgccc agccaatttc ctccatgaag atcctcgtca
     1321 ccggcgctgc cggttttatc ggcatgcaca ccacgctgcg cctgctcgcg cgcggcgacg
     1381 aggtggtcgg cctcgacaac ctgaacgact actacgaccc caccctcaag gaggcgcgcc
     1441 ttgcccggct gcagccgcac gcgggcttcc gcttcgtgaa gatggacgtg gccgaccgcg
     1501 ccggcatgga ggccttgttc gccgccgaga agttcgaccg cgtcatccac ctcgccgccc
     1561 aggccggggt gcgctattcg ctgcagaatc cgcacgccta tgtcgacagc aacctggtcg
     1621 ggttcatgaa catcctcgag ggctgccgcc acgcgaaggt gcagcacctg gtctatgcgt
     1681 cgagctccag cgtgtatggc ggcaacacga agatgccgtt ctcggagagc gacagcgtcg
     1741 accacccggt gagcatctac gcggcgacca agaaggccaa cgagctgatg gcgcacacct
     1801 atagccacct ctacggcctg cccaccaccg ggctgcgctt cttcaccgtg tatggcccct
     1861 ggggccgccc ggacatggcg ctgttcctgt tcaccaaggc gatcctggaa gggcgtccga
     1921 tcgatgtctt caaccacggc cgcatgcggc gcgacttcac ctatgtcgac gacatcgtcg
     1981 agggggtgat ccgcaccctc gatcgcatcg ccgaacccga tcccgccttc gatccgatgc
     2041 agccgaaccc gggcaccggc agtgcaccct accgtgtgtt caatatcggc aaccatgacc
     2101 cggtcgaact gatggagttc gtcgccgcga tcgaggatgc gctcggcacc gccgcgcaga
     2161 agaacttcct gccgctgcag gacggcgacg tcccggccac ctacgccgac accgccgcgc
     2221 tgaacgcgtg gaccggcttc gccccggcga ccagcgtgcg cgagggcgtc ggccgcttca
     2281 tcgcctggta ccgggagtac taccgggtct gatccgcccg gggcggcccg gcgcgacggc
     2341 cgccccccga tctacaacct tccatgcgct gcggcccgcc ggccggcagc ggcaggaatc
     2401 gatggccaac tacgcaatcg gcgacatcca gggctgctac gccccgctgg accgcatgct
     2461 cgaccgcatc gccttcgatc ccgcggtcga ccggctgtgg atcgtcggcg acctcgtcaa
     2521 tcggggcccc gaatccctgc gcgtgctgcg tttcctgcgc gggctgggcg atgctgcgag
     2581 catcgtgctc ggcaaccacg acctctacct gctgatggtc gccgccggct acaagcggcg
     2641 cgacagcgac gacacgctgt tccaggtgct cgaggcggac gaccgcgacg agttgctgga
     2701 gtggctgagc ttccagcccc tgatgcgggt ccatggcgac cacgtgctgg tgcatgccgg
     2761 cctcttgccc gggtggacgg tgacgcgcgc gcaggcgctc gccgccgagg tgggtgcggt
     2821 gctggccggg ccgaagtcgc gcgagttcct gctccacctc gcgggcaacc gcccggatcg
     2881 ctggtcggac gggctcaagg gctgggaccg tctgcgcgtg atcgtcaatg ccatgacgcg
     2941 gctgcgcttc tgtaccgcgc aggggcgcat ggacctgagt gccaagggcg cgccgcacaa
     3001 ggcgccgtcg ggcatgctgc cgtggttccg cgtgcccgac cgtttcagcc gtacccatac
     3061 gatcgtctgc gggcactggt cggcgctcgg cttctaccgc gagccggggc tgatcgcgct
     3121 cgattcgggc tgcgtgtggg gcggcaagct gaccgcgctg cgcatcgagg acgacgcggt
     3181 gttccaggtc ccgggctgag cctgcggcgg tggtctcagg tctgcgcgtc gagcgcgacg
     3241 ccgatggcgg tggcgatctc gccgcgcgtg cgtgcagcca gcgtgcgccg gtccgctccg
     3301 tcggcggtgg cgatcggcgc cgccaccgtg atgcgcgcga tcgtgcgcgg cgcggcgatg
     3361 atgttggcga tgcactcgcc caggctgagc tcgccatcgt aggcgggcgc gcggctgaag
     3421 cggtcgtccg gcgtgcggta ttgcagcgcg aggggctgca ccggatggcc gcaggcgatc
     3481 gccggctgca gcagtgcggc gtggaagtgc agcagggtcg cgccgtcggt ggtcgtaccc
     3541 tcggggaaga tggcgacgtt gcacccggcg tcgagcagcg cggcggtttc ctcgttgatg
     3601 atgcgggcgt ggccacggct gccgcgacgc aggaagatgg tgtcgttctt cgccgccagc
     3661 cagccgatca ccggccaggc gcgtacctcg gacttggaga cgaaggcggc gggggcgagc
     3721 gcgttgatcg cgaagatgtc cacccaggag acgtggttgg caaccagcat gcaaccgggt
     3781 gcgattgcct cgccgcgatg ctcgaggcgc atcccgagca ccctcagcag cccctgagac
     3841 cagcgctggc gcagggcctc gcgagtgtcc cgaccggtga gcggatagat cgtcgcgatc
     3901 gtcagcaccc cctgcagtag gtgcagggcg agccgggtcc agcgcgtcgc gcgcagcagc
     3961 ccaccagcgc gcgcctcgct cgccgcggcc gcgcgggtgg ctgctccgga cgcggaggct
     4021 ggtggcgcgg tgccggcacc ggcatgcatg gcgcgatcct gcacgaggtc ggggtggcgg
     4081 ggggcgttca gtccttgcgt cccatgaagt ggcgggcgta gcgcccatcc acgcggccga
     4141 tcggcatcag gatgggcagg tccgcggtgt tgaagtcggg gtcccatgcc ggctcgccgc
     4201 agacccaggc tcccgcgcgc aggtagccct tgatcagcgg tggcgcctcg gcgtcgaggt
     4261 cgccgcgcag gctcgagagc ggcagcgggc agcgcgggaa gacgtggtac tcgagcgggg
     4321 cgaggtgttt ttcgcgcagg cgattataca ggctggccgc ggcgtgcccg ccatcggcca
     4381 tgcttaccga ggcgcagccg atcaggtatt cgtagccgtt ctcctgcatg tagcgcgcga
     4441 gccccgacca cagcagcgcg atcaccgcgc cgctgcggta gtcggcgtcg atgcacgagc
     4501 gcccgatctc caccagctgg ccgcgcaggt gctgcaggcg ggtgaggtcg aattcgttct
     4561 cggagtagta gccacccacc cggcgtgcgg cggagggcga caggatgcga taggtgccga
     4621 cgatgcggcc ggcatcctcg tcgcgcacga tcaggtgctc gcagaaggga tcgtagaggt
     4681 cgcggtcgac gcccggactg cgtgtgggca ggcgggcccc catctcctcg gcgaagaccc
     4741 ggtagcgcag cttctgcgcc tcgaggatct cggtctcgct gctggcgagg ccgacgtgca
     4801 ggttgcgcgt cttgcgcgcg gcggtttgct ggcggggctg gaggtgctgg agcatggcct
     4861 gtgtccgtcc tgttgggtgc ggaccattct cgaaacccgt cgttgcgacg cggtgacgtg
     4921 ctggttacga tgcgatgacg cacatccggc cccaggagaa cttcgcatgc agtgcacgac
     4981 cccgcccgac cacgaccccg actgcgtgtt ctgccgcatc gcgcgcggcg aactgccgtc
     5041 gtccaaggtg tatgaggacg ccgacacgct cgccatcatg gacatccagt cggtcaatcc
     5101 cggccacatg ctggtgctgg tcaagccgca ccgcgcgaac atctacgcgc tcgacgacac
     5161 cctcgccggc gcggccttcc gcactgcggc gcgggtcgcc cgcgtggcga agaaggtgac
     5221 cggctgcgag ggagtgacgc tgctccaggc caacgagaag gcgggggcgc agaccgtgtt
     5281 ccacttccat ctccacgtgc tgccgcgctg ggaaggcgac ggcatggcgc tggcctggcc
     5341 ggtgaagaac ccgccgcgcg aggcgctcga ggcgatggcg gcgaagctgc gcgaggcgct
     5401 cgccggtcag ggctgaagac ctggccgcgg ctccggcggc ggcaggggaa ggcggagggg
     5461 ccgcgtataa tccggcttcc gcccaccctc cgcctgcctg ccttccatgt ccatcctcgt
     5521 ctgcggatcg gtcgcctacg actcgatcat ggtcttccac gaccgcttcc ggaatcacat
     5581 cctccccgag cagatccata tcctcaacgt ctccttcctg gtgcccgacc tgcgccggga
     5641 gttcggcggc tgtgccggca acatcgccta cgggctcaag ctgctcggca gcgatccgct
     5701 gatcgtcgcc accgtgggcg aggacgccgg tccctaccgt gcgcgcctgc gcgcgctcgg
     5761 gctgcgcgag gacttcgtgc gcgaggtgcc gggcaccttc accgcgcagg ccttcatcac
     5821 caccgacctc gacgacaacc agatcaccgc cttccatccg ggggcgatgt tccactcgca
     5881 cctgaacgac gtgcgcaccg cggccggtgc gacgctgggc atcgtctcgc ccgacggcgc
     5941 cgacggcatg ctgcgccacg ccgacggctt cgccgcggcc ggggtgccct tcgtgttcga
     6001 cccgggccag gccctgccca tccttggtag cgaagcgtta ctgaaatgcc tgcaacaggc
     6061 gcgctactgt acggtcaatg actacgaggc ccggctgatg tgcgagaaga ccgggcgcag
     6121 cgaagcggag ctcgcccgcg aggtggaggc gctggtggtg accttgggag cggaaggttc
     6181 gcgcgtgcat tggcgcggcg aggtgttcga ggtgccggcg gtgagggcgg acgcgatcgt
     6241 cgatccgacc ggttgcggcg acgcctaccg cgccggcctg ctgcacggta tcgccgcggg
     6301 ctgggacctg cgcaggagcg cgcgcctggc cgcggtcatg ggcgcgctca agatcgccca
     6361 gcgtggcgga cagaactacg cgccctcgcg tgcagacgtc ggggcgcgtt atcgcgaggc
     6421 tttcggagag gatctatggt gaatcgaagc gggatgaagt ggcgcgccct ggccggcgcg
     6481 atgatcgcgg cgctggcggt gggcggctgc gcccagggac tggggggtgg cacgtatgcg
     6541 cgcaccgagg cgcggcgcgc gatgacggtg cagttcggca ccatcgaggc ggtacgcgcg
     6601 gtgcagctcg agggaacgaa gacgccgatc ggctcggtcg cgggcgcggc catcggcggt
     6661 atcgccggca acacggtcgg cggcggacgt ggccaggccg tggccaccgt catcggcgcg
     6721 gtggccggcg ggcttgccgg ctctgcggtg gaggaaggcg cgacccggca agcgggcgtg
     6781 gaagtcaccg tgcgtctgga caatgaccag tttctcgccg tggtgcaggc cgacgagggc
     6841 gaaggcttcc gccccggtga gcgcgtccgc gtgctgcgcg acgccggcac cacccgcgtc
     6901 tcgcgctgag aaggatgccg ggcgcgagcc cggcgcatgc ggggtcgagc gcgctcgctc
     6961 ccgctcctca cccctgatgc cttacgcctc actcctcacg gatagaatgc atagatccgg
     7021 tatcccgtcg gtgccagcgc cggtgcggcg aagggaatgc gcaactcgat cgcgctgcgt
     7081 gccatgaagg cttcgtgcgg ctggctcggt ggcagccagt cggtggatgg caacacgcgg
     7141 cgcgagatcg ggttgtcgcc ggcgtcggtg agggtgagtt ccagatgcgg ccactgctgc
     7201 ggatagtccg cgcggttgtt gagcgtggct tgcagcaggt agtagccggg ccgcccgggt
     7261 tcggcctgca ggtcggaggc ctcgatcgcg atgtgttccg catcgcgcgg atagggcacg
     7321 ccgcagccga agcgcgcgca ggcctcgctc aggacggggc gcaggccggg gagggcgcgc
     7381 gccagctcca tgcgatacag ataggtggcc tgcgccgcga gcagcgcgga caagccgacc
     7441 gcgagcagca tcgcgagcgc gcgcacgagc gggtgggttt ccttgcgcgg gcgtccgtag
     7501 ttggcgtcga ggcgggcgag gtcgggctcg tgaggcgaga cggggcgctc atcctcgtgc
     7561 gcagggatgg aggcgggttc cggtgcggcc tgcgcggatt cgcgcacggg ggtggagagc
     7621 ccggggattg cggtggccgg atcgtcgtcg aagggcggcg gtggcgtgta ggagatcgtc
     7681 ggtgcgaagg gcccgggcgc ggcgaggtcc ctgccgcgag caggcgttcc gccctcgtcc
     7741 tcgtcgatcg gcaggcctcg ccgggcgctg cgcaagactt ccggaaaggg ctgggccgct
     7801 gcggcgccct cgtcgaaggc gggggccgtg ccggtctgcc cgtcgtccat cgcggtgccg
     7861 cgcagcgcgg gcttgtgcgc gggcgcgagc cagtcctcgt agtcgtcgag acccggctcg
     7921 ggcagcggcg cccggatcgg cggcgagggg gcaacctcgg ggatgtcgaa gtcgagcagc
     7981 ggatcggggc gtgcttcata caccggtctc gccgcggggg gtgggggcgc cgctgtggcc
     8041 gcggcgccgt cgtcgcgaac ctcgaccgtg tgctcgagcg cgttgaaggg gtggaagcaa
     8101 tgcccgcagc ggacctcgcc gccccgtgcg tgaagctgct ccgggcgcag ccggaacacc
     8161 gtctggcagg cggggcagcg ggtgatcatc atggcccggg gcgccgtccc tcgaggcgga
     8221 tccagccttc gtgcgccgcg cccacgtgca gtgcgatgtg cggtgcccag gcctccacga
     8281 cctgcgcggc ctgggtctcg agcacgcccg acagcgccac ccggccgccc ggagcgaccc
     8341 gcgccgaaat cgccggcgcg agcacgcaca gcgggttggt gaggatgttg gcgacgacca
     8401 gatcgaaggt gtcgccgacc ggcacggcag agtgctgcag gcgtacggcc gcgtggttgc
     8461 gcgcggcgtt gtcgcgcgcg gcctcgaccg ccttctcgtc gatgtcgatg ccgagcaccg
     8521 cgccggcgcc gagcttggcc gcggcgatgc cgaggatgcc cgagccgcag ccgtagtcga
     8581 gcacgctgca gccgggcgtg accacctcgc acagccactc caggcagagc cgggtggtgg
     8641 ggtgggagcc ggtgccgaag gccatgccgg ggtcgagctc gatgttgatc gcgtcggcgt
     8701 cgggcgcctc gtgccaggac ggcacgatcc acagccggtc ggtgatgcgg atgggatcga
     8761 actggctctg ggtgagctgc acccagttct gctcggctac cgtctcggtg gagaacgggg
     8821 ggacggcgtc gaaacccgcc gcgcgggcgg cttcggcgag catcgcgccg agctcggccg
     8881 gatcggccgc accgtcgaac agcgcgatca cccggctgtg gtcccacagc gcggtgggca
     8941 ggtggccggg ctcgccgaac tgcgggcgct ccgcctcggt gccggcgtcg gcgtcctcga
     9001 tcgagacaga gagcgcgccg gcctccatca gtgcgtcgga gagcgcctcg gccttgtcgg
     9061 cctcggcctg cagggtcacc gagatccaca tcgcgcgtca gtcctgcact tcgttgcggt
     9121 gggtgagctt gtgctcgagg taatggatgc tggtgcagcc ctcgttgaag cgcgggtcga
     9181 gcatcagggt ctggtgcagc gggatgttgg tcttgatgcc ctcgaccatc atctcggaga
     9241 gcgcgatgcg catgcgacgg atcgcctgct cgcgagtgtc gccgtatgcg atcagcttgc
     9301 cgatcatcga gtcgtagtgg ggcggcacgg tgtagccgtt gtaaacgtgc gaatcgaccc
     9361 ggatccccgg cccacctggc gtatgccagt tggtgatgcg accgggcgag ggcgtgaact
     9421 tgaacggatc ctcggcgttg atccggcact cgatcgcgtg gccgcggaac tcgatgtcgc
     9481 gctgacggaa ccagagcttc tcgccggcgg cgatgcggat ctgcgcctgc acgatgtcga
     9541 tgccggtgat cagttcggtc accggatgct ccacctgcac ccgcgtgttc atctcgatga
     9601 agtagaactc gccgttctcg tagaggaact cgaaggtgcc ggcgccgcgg tagccgatct
     9661 tgcggcaggc ttccgcgcaa cgttcgccga ccttggcgat cgtcttgcgg tcgatgccgg
     9721 gggccggcgc ttcctcgatc accttctggt ggcggcgctg catggagcag tcgcgttcgc
     9781 cgagatagac cgcgttgccg tgctggtcgg caaggatctg gatctcgacg tggcgcggat
     9841 tctcgaggaa cttctccatg tatacgacgg ggttgccgaa ggccgcctgg gcctcggcgc
     9901 gggtggtggt gacggcgttg agcagcgcgg cctcggtgtg caccacgcgc atgccgcgcc
     9961 cgccgccgcc gccggcggcc ttgatga
//
LOCUS       DAOQNN010000077         9931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3232_length_9931_cov_10.227116, whole genome shotgun sequence.
ACCESSION   DAOQNN010000077 DAOQNN010000000
VERSION     DAOQNN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3232_length_9931_cov_10.227116"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2723
                     /gene="cas3"
                     /locus_tag="PKV97_06250"
     CDS             <1..2723
                     /gene="cas3"
                     /locus_tag="PKV97_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HNB05516.1"
                     /translation="YPGEPKWHPLVYHCLDVAAVGVEYMERAPAQRRLFAVALGDEAG
                     LAGWIAFWLALHDLGKFTEAFQGQRTDLFRALRGRPPGKTYSLRHDSLGWLLWQGLIS
                     GQAETEQWFRPDSDAYRDGLDAWVRAVTGHHGQPPLSGGFWKTHCHRQEDCAAVQDAA
                     TALRGLLLDEAACQIPARLGAERFAEISRDLSWWLAGLTVLADWIGSNTRYFPYQSAK
                     QSLADYWQQARKQAAFALSDCGVLPVRSRSELPFGKLFPNIALPSPLQCWAADLDLPA
                     GPQIHLLEDVTGAGKTEAAVVLAHRLMAQGQAEGFFIGLPTMATANAMYGRIAAVYGR
                     LFEGEASLALAHGHRRFVEAFARSVLPPTTAEHDSAQQDETASARCAAWLADHNKRAL
                     LAPAGVGTLDQALLAVLQSRHQSLRLLGLSHKVLLVDEVHACDDYMLGVLCVLLQFHA
                     RAGGSAILLSATLPVAMKRKLLAAFAHGCGNTNIPALTQMAYPLATSWHSGGPQRLLE
                     TPLDTRSSVMRTVTVRSAEDQEELIAAVLAALDAGRCVCWIRNTVADALDAFELFRGR
                     IPDEHLMLFHARFALCDRLDIEARVLMHFGPESSPAERAGRLLIATQVVEQSLDLDFD
                     LLVTDLAPIDRMIQRAGRLCRHVRDEAGRRLIAPQAMDRRGSPCLWVYGPAWTETPAG
                     DWFKRTFPKAAVVYPDHDQLWLTAQALRRGSIAMPQDARRLIESVFGEDAQTPEGLQH
                     SADRAQAKGYADASQARANTLTFEAGYSADGTDWWSEARTPSRLGEPTANVVLARWDG
                     DSLRPWADHDDPRQAWAYSTVRVAERLIARAVEPESASRKAALEAVREALPGQGRWSV
                     LLPLDETRDGWQADAVGSPEPGVFRDKRIRYSRRAGLVGGDVLDGDPSST"
     gene            3079..4692
                     /gene="casA"
                     /locus_tag="PKV97_06255"
     CDS             3079..4692
                     /gene="casA"
                     /locus_tag="PKV97_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HNB05517.1"
                     /translation="MNLLDEPWLPVRDAGNHRHWIAPAALGDPAWIAFDADRADFNGA
                     LTQFAIGLLQTAAPVKNAAQWRGWFRQPPDATTLDDWFAPLRPAFEFDGNGARFMQDR
                     TLASNEGACNDIGALLIEAPGEQTLKNNADHFIKRGQITALCPTCAATALLTLQINAP
                     SGGAGHRTGLRGGGPLTTLVACQPARTLWHDLWLNVLEQEVFLAQAGDARLARTHFTF
                     PWMADIATLQKEKGELAPIQTHPAHVHWAMPRRIRLDFSDTVAGDCGVCGRYSDTLLQ
                     RYVTRNYGLNYKGAWNHPLSPYYQNGEDWLPMHPQPGGIGYRHWLGWVLGQTEGKKSV
                     RPARVVSHLCERRRRDVEGQLRLWAFGYDMDNMKARCWYEAQLPLYGLAECETDAQRE
                     VEEAVRPWLAGAELTVFFLRSAVKQAWFDDEARGDFSAIDASFWSGTELPFYRLLRQA
                     IDHARLDREIDAGAFATGWHRQLRQIAIDLFDRQFVGAGAVERQNPRRVAEAHRQLQR
                     NLDGKKLRTALGLPIQIKANPTETEHALT"
     gene            4702..>4916
                     /locus_tag="PKV97_06260"
     CDS             4702..>4916
                     /locus_tag="PKV97_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05518.1"
                     /translation="MNRDFQKNPRLHAALHDWWAGLKDERGDRAQLRRAHDITAIILS
                     PAYQRAHRRLCAAGWEVEGNSPLNDRLA"
     assembly_gap    4917..5016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5077..6144
                     /gene="cas7e"
                     /locus_tag="PKV97_06265"
     CDS             5077..6144
                     /gene="cas7e"
                     /locus_tag="PKV97_06265"
                     /inference="COORDINATES: protein motif:HMM:NF020902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HNB05519.1"
                     /translation="MSRFVQLHLLTSYPPANLNRDDTGRPKTALVGEALRLRISSQSL
                     KRAWRTSGIFQEALVGHIGTRTKRMGTEIFAALREHGIAEKAAREWTRAIASQFGKIK
                     SEKKTEHNEDLEIEQLAHFGPEEREAVMALARNCAERGSGPSEEELDLLRKPERAADI
                     AMFGRMLADSPRYNVEAAVQVAHAVTVHKAAVEDDYFSAVDDLNDGREDAGAGHIGER
                     GYGAGVFYLYLCINRELLQENLGGDAVLTGRALEALVHAVTKVAPTGMQNSFASRAYA
                     AFALVEEGDQQPRSLAQAFLKPVRAREDLSLIDAAVAALDRRCANFDQVYGACADGRY
                     RFNVETGEGSLQGLTDFVKGV"
     gene            6147..6905
                     /gene="cas5e"
                     /locus_tag="PKV97_06270"
     CDS             6147..6905
                     /gene="cas5e"
                     /locus_tag="PKV97_06270"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HNB05520.1"
                     /translation="MDFLLFQLHAPLAAWGDTAVGEYRGSLNWPSESALIGLLGAALG
                     VRRTDEAAHAGLRQGYRYAVGTVAPGTLLRDYHTAQVPGRADLKKRPHQTRRDELAVP
                     KHLLNTVLSTRDYRQEGAWRVAIQEAQNPPHSLQTLAEALAKPHFVLYLGRKSCPLAA
                     PLDPWLIAAGSVKDAFEAYRLRQHEHLQSQRNRHGELPLESPEPLRQMAWPDQMEAGM
                     EPDLTTVRKDRLIRRGGWQFADRFEHVAMLSGEE"
     gene            6908..7606
                     /gene="cas6e"
                     /locus_tag="PKV97_06275"
     CDS             6908..7606
                     /gene="cas6e"
                     /locus_tag="PKV97_06275"
                     /inference="COORDINATES: protein motif:HMM:NF020377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HNB05521.1"
                     /translation="MFFSLIAPTPGSERDAAHALLGGPYEEHQWLWRFFPGNPDARRD
                     FLFRRSEQTGMARYHVVSARQPVSPDSAWMVQSREYAPRIAPGTRLQFELRANPVVRT
                     RRDGKACRDDVVMAEKKRLLQARGLDRWADWVDADRPALYDLVAQTCSLWLHRSGERN
                     GFALDEEALRVDAYQNHAEKRQLPRERRLRFSSVDFAGVLTVTDTDRLHEALIHGIGP
                     AKAFGCGLLLVRRL"
     gene            7665..7901
                     /locus_tag="PKV97_06280"
     CDS             7665..7901
                     /locus_tag="PKV97_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNB05522.1"
                     /translation="MEATLTSKGQVTIPKAVRDALHLRSGDRLDFILEADGTVRVLPI
                     TGSVKRLKGMLPKPPRPLTVEEMDEAIAKGAAGA"
     gene            7898..8296
                     /locus_tag="PKV97_06285"
     CDS             7898..8296
                     /locus_tag="PKV97_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNB05523.1"
                     /translation="MIGLDTNVLVRYIVRDDPAQTALADHCIDHRCSREQPGYVSHLV
                     MAELCWVLERGYGYPRQILGEVLGTLLTSEEIKIQDAPRVRLALGAFSAGAVDFADCL
                     IGLLHQRAECETTVTFDKKAARLPTHRLLE"
     gene            8430..8711
                     /locus_tag="PKV97_06290"
     CDS             8430..8711
                     /locus_tag="PKV97_06290"
                     /inference="COORDINATES: protein motif:HMM:NF041551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HNB05524.1"
                     /translation="MKTTTLPSVRVAGELRAAVESVLCEGETLSAFLLESVRLNVERR
                     EAQREFITRGLRARDEARASSEYVGTEEMLARLDATLAKARERLAGESR"
     gene            8708..9007
                     /locus_tag="PKV97_06295"
     CDS             8708..9007
                     /locus_tag="PKV97_06295"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNB05525.1"
                     /translation="MNYRLRLTPGAAEDLQRLFDFLAEQDLAMAERARVAIAKAFDFL
                     REFPFACRKIGAEHPLLREMVISFGRAGYVALYEIESERTVTVLAVRHQREDDYF"
     gene            9007..9927
                     /gene="cas1e"
                     /locus_tag="PKV97_06300"
     CDS             9007..9927
                     /gene="cas1e"
                     /locus_tag="PKV97_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HNB05526.1"
                     /translation="MADLLPPLRPIPIKERLSVLYIERGQIDVLDGAFVVVDANGVRT
                     HIPVGGVACLMLEPGARVSHAAVALAARVGTLLVWIGEAGVRLYSAGQPGGARSDRLL
                     YQAKLALDPDLRLKVVRKMYELRFGEPPPQRRSVEQLRGIEGARVREMYKRIAQKFGV
                     TWRARNYDPTEWDASDLPNRCLSSATACLYGVTEAAVLAAGYAPAVGFIHTGKPLSFV
                     YDVADVYKFETVVPVAFQVAAKKPANPEREVRLRCRDIFRQTRLLERIIPGIEEMLAA
                     GEIAPPVAPDDAIGPAIPNPEPLGDAGHRG"
BASE COUNT         1742 a         3133 c         3236 g         1720 t
ORIGIN      
        1 gctaccccgg cgagcccaag tggcacccgc tggtttatca ctgcctggac gtggcagcgg
       61 tgggggtaga gtacatggag cgcgcgccgg cacagcggcg gctgtttgct gtggcactgg
      121 gggatgaggc cggactcgcg gggtggattg ccttttggct tgcgctgcat gacttgggca
      181 agttcaccga ggcgtttcag ggccagcgca cggacctgtt ccgggcgctg cgcggacgcc
      241 cgccgggcaa aacctacagc ctgcgtcatg acagcttggg ctggctgctc tggcaggggt
      301 taatcagcgg gcaggccgag acggagcagt ggttccggcc ggacagcgac gcataccgcg
      361 atggactcga cgcctgggtg cgcgcggtca ctggccatca cggacagcca ccgctttcgg
      421 gcggattctg gaaaacccac tgtcaccgac aggaagattg cgcggcggta caggatgcgg
      481 cgacggcgct acgaggcctg ctgctcgatg aggcagcctg ccagatacct gcgcggctcg
      541 gtgcggagcg ctttgccgag atcagccggg atctgtcctg gtggctggcc gggcttacgg
      601 tgctggccga ctggatcggt tccaacaccc ggtattttcc gtaccagagc gccaaacaat
      661 cgttggccga ctattggcag caggcgcgca agcaggctgc cttcgcgctg agcgactgcg
      721 gcgtgttgcc ggtgcgcagc cgtagtgaac tccccttcgg caagctgttc ccgaatatcg
      781 ccctgccttc gccgttgcag tgctgggccg ctgacctcga cctgcccgcc ggcccgcaga
      841 ttcacctgct ggaggatgtc accggcgccg gcaagaccga agcggcggtg gtgctcgccc
      901 accgcttgat ggcgcaaggg caggctgagg ggtttttcat cggcctgccc accatggcca
      961 ccgccaacgc catgtatggc cgtatcgcgg ccgtttatgg gcgcctgttc gaaggtgagg
     1021 cgagtcttgc gttggcccat ggccaccgcc ggttcgtcga ggcattcgcc cggtcggtgc
     1081 taccccccac caccgcggag catgacagtg ctcaacagga cgaaaccgcc agtgcacgct
     1141 gtgcggcctg gctcgccgac cacaacaagc gggcgctgct ggcccccgct ggcgtaggaa
     1201 cgctggacca ggccttgctc gcggtgttgc agagccgcca tcaatcgctg cgcctgctgg
     1261 gtttgagcca caaggtgctg ctggtcgatg aagtccatgc ctgcgacgac tatatgctgg
     1321 gcgtgctctg cgtgctgctg caatttcacg cccgcgccgg tggcagcgcg attctgcttt
     1381 cggccacctt gcccgttgcc atgaagcgca agctgctggc cgcctttgcc catggctgcg
     1441 gtaacacgaa tattcctgcg ctgacgcaga tggcctatcc gctggccacg agttggcaca
     1501 gcggcgggcc gcagcgactg ctcgaaaccc cgctcgacac gcgctcctca gtgatgcgca
     1561 cggtgacggt gcgctcagcg gaagatcagg aagagctcat cgccgccgtt ctggctgcgc
     1621 tggatgccgg gcgctgtgtc tgctggattc gcaatacggt ggccgatgcg ctcgatgcct
     1681 tcgagctgtt ccgcggccgg attcccgatg aacacctgat gctgttccac gcccgtttcg
     1741 cgctgtgcga tcgcctggac atcgaagcgc gggtgctcat gcatttcggc cccgaaagca
     1801 gtcctgccga gcgcgccgga cggctgctca tcgccaccca ggtggtcgag cagtcgctcg
     1861 atctcgactt cgatctactg gtcaccgatc tggcgcccat tgaccgcatg atccagcgcg
     1921 ctggccgcct gtgtcgccat gtgcgtgatg aggcgggtcg gcgtttgatc gcgccgcagg
     1981 caatggatcg gcgcggttcg ccgtgcctgt gggtctatgg accagcctgg acggaaaccc
     2041 cggcaggcga ctggttcaag cgcacattcc ccaaagccgc ggtggtctat ccggatcacg
     2101 atcagctgtg gctgacggcc caggcgctgc gccgcggttc gatcgccatg ccgcaggacg
     2161 cgcgcaggct gatcgaaagc gtttttggcg aggacgcgca gacgcccgag gggctgcaac
     2221 acagcgccga ccgtgcccag gccaagggtt atgccgatgc cagccaggcg cgggccaaca
     2281 ccctcacctt cgaggctggc tacagcgccg atgggacgga ctggtggagt gaagccagaa
     2341 cgccctcccg gctcggcgag cccacggcca acgtggtgct cgcgcgctgg gacggcgaca
     2401 gcctgcggcc ctgggccgac catgacgatc cccgtcaagc ctgggcctac agcacggtgc
     2461 gagtggcgga acgcctgatc gcccgcgcag tcgagcccga atcggcctcg cgcaaagccg
     2521 ccctcgaggc ggtgcgcgaa gccctgccgg gacaggggcg ttggtcggtg ttgctgccgc
     2581 tggatgaaac gcgcgacggg tggcaggcgg acgcagtggg ctcccctgag ccaggcgtat
     2641 ttcgcgacaa gcgaatccgc tattcgaggc gtgcaggtct ggtcggaggg gatgtgctgg
     2701 atggtgatcc atccagcaca tgagccccgc gtaggcgggg aacgtatcgc cgctggccgg
     2761 attggtaagc cgacatgcgg ttcatctccg gcttacggag aactttggca attataagtt
     2821 ttagaaaaca aatgtagcgg agttaagctt caaataattt ttttggaaaa ccaacatgct
     2881 tgactatatt cgcattgccc ggcacagtgc agcttgtggc ctctgggcaa gaccgaagac
     2941 tcgaaggaaa ccgcctccaa acggctccct gcttcggatg ttgccgtgag cggacggcgt
     3001 ccaaaaccgt cctgaccacg ccgttccccg cgcgagcggg gatgagcgtc agtaagcaga
     3061 acgtattgag gtaggacgat gaatctgctc gatgaaccct ggctgccggt gcgcgatgcc
     3121 gggaaccacc gccactggat tgcccccgcc gcgctgggcg atcccgcctg gattgccttc
     3181 gatgccgacc gggcggactt caatggcgca ctgacgcagt ttgccatcgg cctgctgcaa
     3241 accgccgcgc cggtcaagaa cgccgcgcag tggcgcggat ggtttcggca gccaccggac
     3301 gccacgaccc tggacgactg gttcgcgccg ttgcggcccg ccttcgagtt tgacggcaat
     3361 ggcgcgcgtt tcatgcagga ccgtacgctc gcttcgaatg aaggtgcctg caacgacatc
     3421 ggtgcattgc tgatcgaggc ccctggcgag cagacgctga agaacaatgc cgaccacttc
     3481 atcaagcgtg gccagatcac cgccctgtgt cccacctgtg ccgccaccgc gctgctgacg
     3541 cttcagatca atgcgccatc gggtggcgcc ggccatcgca ctgggttgcg gggtggcggc
     3601 ccgctgacca cgctggtggc ctgccagccg gcacgcactc tgtggcacga cctgtggctg
     3661 aacgtgctgg agcaggaggt ttttctggct caagccggcg atgcccgtct tgcccggacg
     3721 cacttcacct tcccatggat ggcagacatc gccaccctgc aaaaggaaaa aggagagctg
     3781 gcacccatcc agacccaccc ggcgcacgtc cattgggcga tgccgcggcg catccgcctc
     3841 gatttcagcg acacggttgc cggcgactgc ggcgtctgcg gccgctactc cgacaccctg
     3901 ctgcagcgct atgtcaccag gaactacggg ctcaactaca agggcgcctg gaatcacccg
     3961 ttgtcgcctt actaccagaa cggcgaggac tggctgccga tgcatccgca gccgggcggc
     4021 atcggctatc gccactggct cggttgggta ctgggccaga ccgagggcaa aaagtccgtt
     4081 cgcccggcac gggtggtgtc acacctgtgc gaacgccgcc gccgcgacgt cgaaggtcaa
     4141 ctgcggctgt gggcctttgg ctatgacatg gacaacatga aggcgcgctg ttggtacgaa
     4201 gcccagttgc cgctgtatgg cctggccgaa tgcgagaccg atgcccagcg cgaggtggaa
     4261 gaggcggtga ggccctggtt ggctggggcg gaactgacgg tgttttttct gcgcagcgcg
     4321 gtcaagcaag cctggttcga tgacgaggcg cggggtgatt tttcggcgat cgacgccagc
     4381 ttctggagtg gtaccgaact tcccttctat cgcctgctgc gccaagcgat cgaccatgct
     4441 cgcctggatc gtgagatcga tgccggggct tttgccaccg gatggcaccg gcagttgcgg
     4501 cagatcgcaa tcgatctgtt cgatcggcaa ttcgtcggcg ctggcgccgt cgagcggcag
     4561 aacccgcgcc gcgtggccga agcccatcgc cagttgcagc gcaatctgga tggcaagaag
     4621 ctgcggaccg ctttgggatt gccaatccag atcaaagcca atccgaccga aaccgaacat
     4681 gcactgacct agggaggcgg catgaaccgc gattttcaga aaaacccgcg cctgcacgca
     4741 gcactgcacg actggtgggc cggcttgaag gatgagcgtg gtgaccgcgc acaactgcgc
     4801 cgcgcccatg acatcaccgc catcatcctc agccccgcct accagcgcgc ccatcggcgc
     4861 ctgtgcgcgg cgggttggga ggtcgagggc aactcaccgc tcaacgaccg gctggcnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngact acgaatggcc tgcaccgaaa
     5041 gccgactgaa tgccaacttc acacggagac cgaaccatga gccgtttcgt ccaattgcat
     5101 ctgctgacca gctatccgcc cgccaacctc aaccgcgacg acaccggacg ccccaagacg
     5161 gcgctggtgg gggaagcgct gcgattgcgg atttcgtcgc aaagcctgaa gcgcgcctgg
     5221 cgcacttcag gcatttttca ggaagccctg gtcgggcaca tcggcacgcg caccaaacgg
     5281 atgggcacgg agattttcgc agcactgcgt gaacacggca ttgccgaaaa ggctgcccga
     5341 gaatggacac gggcgattgc cagccagttc ggcaagatca aatccgaaaa gaaaaccgaa
     5401 cacaacgagg atctcgaaat cgagcagttg gcccacttcg gtccggaaga gcgcgaagcc
     5461 gtcatggccc tggcacgcaa ctgtgcagag cgcggcagcg gccccagcga ggaagagctc
     5521 gacctgctgc gcaaaccgga gcgggcggcg gacatcgcca tgttcgggcg catgttggcc
     5581 gattcgccac gctacaacgt ggaggcggct gtgcaggtcg cccacgccgt caccgtacac
     5641 aaggcggcag tggaggatga ctatttcagc gccgtggatg atctcaacga cggccgcgag
     5701 gatgccggcg ctggccacat cggtgagcgc ggctatggtg ccggcgtctt ttacctctat
     5761 ctgtgcatca accgcgaact gctgcaagaa aacctcggcg gcgatgccgt gctcaccggg
     5821 cgtgcgctgg aggcactggt ccatgcggtg accaaggtcg cgccgaccgg catgcagaac
     5881 agcttcgctt cgcgtgccta tgcggctttt gcactcgtcg aagaaggcga ccagcaacca
     5941 cgctcgctgg cgcaggcctt cctgaagccg gtgcgcgccc gcgaggatct gagtctgatc
     6001 gacgccgccg tcgccgcact ggatcgccgc tgcgcgaatt tcgatcaggt ttatggtgcc
     6061 tgtgccgatg gccgttaccg cttcaacgtc gagaccggtg agggctccct gcaaggactg
     6121 acggacttcg tcaagggagt ctgaccatgg actttctgct ctttcaactg cacgcacccc
     6181 tggcggcttg gggtgatacg gcggtgggcg aataccgcgg cagcctcaac tggccaagcg
     6241 aatcggcctt gatcggactg cttggcgccg ccttgggcgt ccgtcggacg gatgaagccg
     6301 cgcacgccgg gctgcgacag ggctatcgct acgccgtcgg aacggtggcg ccgggaacgt
     6361 tactgcgcga ctaccacacc gcgcaggtac ccggccgcgc ggacctgaaa aagcggccgc
     6421 accagacgcg acgcgatgag ttggccgtac ctaagcacct gctcaatacg gtgctctcca
     6481 cccgcgacta tcgccaggaa ggcgcctggc gcgtcgcgat acaggaagcg caaaacccgc
     6541 cgcattccct gcaaacactg gcggaagcat tggcgaaacc acacttcgtg ctctatctgg
     6601 gacggaaatc ctgcccattg gcggcaccgc ttgatccgtg gctcattgcc gccgggagcg
     6661 tcaaggatgc ttttgaggcc taccggctcc ggcaacacga gcatctgcag agccagcgca
     6721 accgtcatgg agaactgcca ctggagagcc cagagccgct gcggcagatg gcttggcccg
     6781 atcagatgga agccggcatg gaacccgacc tcactactgt gcgcaaggat cgcctcatcc
     6841 ggcgcggtgg ttggcaattc gccgaccgct tcgagcatgt ggcaatgcta agcggggagg
     6901 agtagcgatg tttttcagcc tcattgcgcc gaccccaggc agcgagcgcg acgccgccca
     6961 tgccttgctg ggcgggccgt atgaagaaca tcagtggctg tggcggttct ttccgggtaa
     7021 tcccgatgcc cggcgcgact ttctgttccg gcgcagcgaa cagacgggga tggcgcgtta
     7081 ccacgtggtg tcggcacgtc aacccgtgag cccagacagc gcctggatgg tacagagccg
     7141 ggagtacgcg ccgcgcatcg ctccgggaac gcgcctgcaa ttcgagttgc gcgccaaccc
     7201 ggtggtgcgc acccggcgcg atggcaaggc ctgccgggac gatgtggtca tggcggagaa
     7261 aaagcggctg ctacaagccc gtgggctgga ccggtgggca gattgggtcg acgcagaccg
     7321 tccggccctt tatgacctgg tggcgcaaac ctgcagtttg tggctgcaca ggtctggtga
     7381 acgtaacggg tttgcattgg atgaagaggc gctgcgcgtg gacgcttacc agaaccacgc
     7441 cgaaaagcgg cagcttccca gagagcggcg cctgcgtttt tcctcggtcg attttgccgg
     7501 tgtgctgacc gtcaccgaca ccgatcgcct gcacgaggcg ttgatccacg gcatcggccc
     7561 cgccaaggcc ttcggctgcg gcctgctgct ggtacggcgc ttgtaggcct gccaggggcg
     7621 gcctatcctt actgcaatgt aaggaatttg tcaggaggaa agaaatggaa gcgacgctga
     7681 ccagcaaagg ccaggtcacc atccccaagg cggtgcgcga tgcgttgcac ctgcgttccg
     7741 gtgatcggct cgatttcatc ctggaagccg acggcaccgt gcgggtgctg ccgatcaccg
     7801 gctccgtcaa acgcctcaag ggcatgctgc ccaagcctcc gcggcctttg actgtcgagg
     7861 aaatggacga agccatcgcc aagggtgcgg ccggcgcatg atcggcctcg ataccaacgt
     7921 gctggtgcgc tacatcgtgc gggacgaccc ggcgcagacc gccttggccg accactgcat
     7981 cgaccacagg tgcagccgcg agcagcctgg ttatgtctca cacctggtca tggcggagct
     8041 ttgctgggtg ctggagcgcg gttacggcta cccacggcag atactgggag aggtgctcgg
     8101 cacgctgctc acgagcgagg agatcaagat tcaggatgcc ccgcgggtgc ggctggcgct
     8161 cggcgccttt tcggccggcg cggtcgactt tgccgattgc ttgatcggtc tactgcacca
     8221 gcgggctgaa tgcgaaacca cggtgacctt cgacaagaag gccgcgcgac tcccgaccca
     8281 tcgcctgctg gagtgagccg tggccgatct gctgccagcg tctccaaggc tctcgatcac
     8341 acgcccgctg gggttgcagg gaatgaaact tgtccgagaa gatcgccaag gctatgctgc
     8401 atcgcaaatg caatgcatga ggagtctgca tgaaaaccac caccctgccc tcggtacggg
     8461 ttgccggaga actgcgtgcc gccgttgaat cggtgctgtg cgagggtgag acactttctg
     8521 catttttgct ggagtcggtg cggctcaacg tcgagcggcg cgaggcgcag cgcgagttca
     8581 tcacccgtgg cttgcgcgcg cgtgacgagg cgcgcgcaag cagtgagtac gtcggcacgg
     8641 aggagatgct cgccagattg gacgccacgc tcgccaaggc gcgggagcgg ctggcgggcg
     8701 aatcccggtg aactaccggc tgcgcctgac gcccggtgcg gccgaagacc tccagcgtct
     8761 gttcgatttt ctggcggaac aggatctcgc catggccgag cgcgccaggg tggccattgc
     8821 caaggcattc gattttctgc gggagttccc ctttgcctgc cgcaagatcg gggcggaaca
     8881 ccccttgctg cgcgagatgg tgatcagctt cgggcgggca gggtatgtgg ccctgtacga
     8941 gattgaaagc gagcggacgg tgaccgtgct cgccgtccgc caccagcgcg aggacgatta
     9001 tttctgatgg ccgacctgct gccgccactg cgcccgatcc cgatcaagga acgcctgtcg
     9061 gtgctctaca tcgagcgcgg ccagatcgac gtgctcgacg gcgccttcgt ggtggtcgat
     9121 gccaacggcg tgcgcaccca tatcccggtc ggtggcgtgg cctgcctgat gctggagccc
     9181 ggtgcccgcg tatcccatgc cgccgtggcg ctggccgcgc gggtcggcac cctgctggtc
     9241 tggatcggcg aggccggcgt gcggctctat tccgcgggtc agcccggcgg tgcgcgttct
     9301 gaccgcttgt tgtatcaggc caagctggcg ttggacccag acctgcggct gaaggtggtg
     9361 cgcaagatgt acgaactgcg ctttggcgag ccgccgccgc agcgccgctc ggtcgagcag
     9421 ctgcgcggca tcgagggcgc ccgcgtgcgc gagatgtaca agcgcatcgc gcagaaattc
     9481 ggcgtcacct ggcgcgcgcg taactatgat ccaaccgagt gggacgcctc cgacctgccc
     9541 aaccgctgcc tgtcatcggc caccgcctgc ctttacggcg tcaccgaagc ggccgtgctg
     9601 gcagccggat acgcgccagc ggtagggttc atccacaccg gcaagccgtt gtcgttcgtc
     9661 tacgatgtcg ccgacgtgta caaattcgag accgtcgtgc cggtcgcctt tcaggttgcc
     9721 gccaaaaagc cggccaaccc cgaacgcgaa gtgcgcctgc gctgccgcga catctttcgg
     9781 cagacccgcc tgctcgaacg catcattccc ggcatcgaag agatgctggc cgccggagag
     9841 atcgcgcctc cggttgcccc ggacgacgcc atcggcccgg ccatccccaa cccggagccg
     9901 ctcggcgatg ctggtcatcg tggttgaaaa t
//
LOCUS       DAOQNN010000078         9785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3296_length_9785_cov_8.920863, whole genome shotgun sequence.
ACCESSION   DAOQNN010000078 DAOQNN010000000
VERSION     DAOQNN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3296_length_9785_cov_8.920863"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1403)
                     /locus_tag="PKV97_06305"
     CDS             complement(6..1403)
                     /locus_tag="PKV97_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004319523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB05527.1"
                     /translation="MTDRPITDRHVLIVDDEELYRELLGGRLGRQGYRISEAGDGEGA
                     LRCIEGGGVELALIDIKMPGMDGIELLERIKQLAPRTEVVVLTGHGSIDSAIAAMKLG
                     AFDYLTKPYQLTELDLVVARALERSALARRCDALSVEVARLRSRDDDGMVGDSPAWLR
                     MLEQVRRAAALDVPVLLTGESGAGKEVVAAALHRWSRRAGEAYVPVNCGLLEGELVES
                     ELFGHKRGAFTGASTDREGLFELASAGTLFLDEIGELPLACQAKLLRVLDSGEFRPLG
                     ASAVRRTGARVIAATHRDLEALVTAGRFRHDLLYRLDVVHIAVPPLRERREDIPLLVE
                     HLLHQAVRRHPGTPAPRVEPQALQRLIDHAWPGNVRELRNVVERLLVFADGDRIDAAR
                     VDALLHPGARTVASTGRTGFERIVPLAEMEQAYLRWVVDKLDGNVSAAATRLGIARST
                     IYRLLRPHADEGARP"
     gene            complement(1396..3540)
                     /locus_tag="PKV97_06310"
     CDS             complement(1396..3540)
                     /locus_tag="PKV97_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR N-terminal domain-containing protein"
                     /protein_id="HNB05528.1"
                     /translation="MRSDELDFHALIDFMPDSGAIRFCGRRAILVHADSMGALRKELV
                     ETLGSEIAKVVLTRYGFSCGYEDAELLAAYMHPDSLEEFILGGPRTHMFSGIAEVAPD
                     ALELDHASGRYRMSGRWRHSYEAEQHLRLFGRADEPVCWTLTGYASGFASRVLQADMI
                     CVETACEGRGDPHCAWTLMNASDCAPELAGLREYFKPLNLRDRLNLLESRVHERTREL
                     EASEQRYRNLIEDLPEMAFALHASGRLLHLNKAARLRLGVSEARLHALRLKDLVLPDY
                     TARATAFLKEIAARRSTTTLDLVMRDAAGLPFPVRLQVEPVLKDDRIVGYSGLALDIS
                     AQLERERELTEYARRIESREQQIQDVINEAVYILDGQGRLSFVNTRMAELLGAAPEAA
                     IGHAVGDFMPASSAARIERDFARRIAGEAGRPFEIALSGREGSSVVLEVNSTLFVGRD
                     VAEGVIGVARDITARRQMERELAQANRLSALGQFASGVAHEINNPLGLVSGYAEELQA
                     LLDECTPLGDDRRLAQLRRGLATIQQQAHRCKAITANLLAFSRRQSASLETVDVGLFV
                     AERLAFFNDAGLTRGVELAVDIAPGLPQAGTSPALLEQVLQNLIKNACDAMNGSGRID
                     IAARAVGEGIEIEIGDSGPGFAPGVAERVFDPFFTTKPPGKGTGLGLSICYAIINELG
                     GRIACGNRPQGGAWFRLVLPLAEDGPGAAKHD"
     gene            3787..6423
                     /locus_tag="PKV97_06315"
     CDS             3787..6423
                     /locus_tag="PKV97_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB05529.1"
                     /translation="MIDPGLPADEPERLAELRALAILDTPREDAFDNLMQLAASIFGV
                     PIALVSLVDEARQWFKSAHGLCVAQTPRDVSFCTHVVFSNAPLVVEDAARDPRFADNP
                     LVTAAPQVRFYAGEPLRSDQGFVLGTLCVIDTVPRRFEEAERARLRLLARQAEQLIRL
                     HRKRQRLADEARRSARIAARYQAITEGAAAGILRIDGRGRIIEVNRFACALFGYEEAE
                     LRGHNVRMLMPEPFRSAHDGHLEAYQRTGVARVIGKGTEVDALHRDGRHIPVQLTVSE
                     VSQGAQADDFEGRHFIGILSDLRDVHQVRERERQERALLEVLHRGLTDYHALISGNTL
                     WGFLKDALCALTGSAYGLIGEVEHGEGGPALKIHAITDLSWNDETRGLMRKLVSGDMR
                     LSNPASMLGRVFARGEVVLSNAMRSDPRGGAMPPGHPPLSRYLGVPIMDRGEVIGMFA
                     IANAPQDYPDELVDWLKPFTSTCALLINLYRLFKEQQRFTEELQQARDQAEKANQAKT
                     EFLSSMSHELRTPLNAILGFAQLLQNGRQPLSERQLRQVEQIARSGRHLLQLINEVLD
                     LARIESGRLQVSLEPMLAHDVIEDAAQIVLPLAEQQGIALALPPAQACALRFTGDYTR
                     VKQILINLLGNAIKYNRPGGAVEVRCEAVGGRCRIGVVDTGIGIPASRLGELFQPFNR
                     LGAESGSIEGTGVGLALTRKLVDLMHGELGVHSREGEGSAFWFELPLCEQVPAVRDEA
                     RAAAAPQALSRRHRVLHVEDNPANRQLMQDVFEDVGQAVLHSVDSAEAGIEAACSDPP
                     DLILMDIDLPGMSGLEARALLARNPLTAHIPVLAISAAASPHTVRKAREAGFIDYVTK
                     PFDVPALVARLGQILEHRSSDD"
     gene            6416..8182
                     /locus_tag="PKV97_06320"
     CDS             6416..8182
                     /locus_tag="PKV97_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB05530.1"
                     /translation="MTDRVPMQADADILIVDDNPANVELLLALLEDAGYLRLEGITDP
                     RRVAARVAEHAPDLILLDVRMPHLNGLELMERLSAGGAEAPAVIILTAQIDDATRYRA
                     LELGARDFLTKPFDQVEVLQRIRNVLHMQRLMKERAERAELLEALVAERTQELVARAR
                     HDPLTGLPNRLSLLDELGARLAARRDAAVLFLSIEGMDEVSRLHGFGVADELARAAAR
                     RLRECVPEGFLAVWNSTQWVLLCDCAPARGATGALAERLLAGFRPPVEVEHMRLHLGL
                     RIGIAAEADERSPEQLVRMAALALPAGEGQWQAYEAELETRMQRRNGIREALRGAVGR
                     DEFSLVYQPKVELCSGDIVGVEALLRWDSSAYGRVSPAEFVPIAEASGEILAIGGWVV
                     RAALATLRRWRQAGRVADVFGMAINVSTQQLMQPDFAERLVAEVEASGVPPAALELEV
                     TESGLMQDMALAARHLDHLARAGFRIAIDDFGTGHSSLAYLKALPVSVLKIDRAFIRE
                     LHANPADQRLTGTVIDMARHFEFITVAEGVEQEAQLERLMDMGCDLVQGFMFAPPLKE
                     DALFRLMKTGFAHMPAFGGAVA"
     gene            complement(8557..>9785)
                     /gene="recD"
                     /locus_tag="PKV97_06325"
     CDS             complement(8557..>9785)
                     /gene="recD"
                     /locus_tag="PKV97_06325"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004319516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HNB05531.1"
                     /translation="GQRSLGGHLDLAALTRRIGAARTLHALLGARPDTRRFRHHAGNP
                     LDVDVVIVDEASMVHLEMMAALLEALPHGARLVLLGDKDQLASVEAGAVLGDLCRDAS
                     DGRYDAETARYLLATTGEALPTRYLEPTDKAPALAQHTVMLRESRRFEGPIGALAHAV
                     NAARDPALPARLLREDASGALHGVEGGPVAAVIALALGGRRGAPACYRDYLELLARGP
                     AGKDAEAHAAWATAILHAFDRFRVLCAVREGDWGMAGLNRAIEHALARARLLSPRGTW
                     YAGRPVMVTRNDPALGVFNGDIGITLPALESGGLRVHFLDGEALRSVAASRLAHVETA
                     FAMTVHKSQGSEFEHTVLVLAAQSGNVLVRELIYTAITRARKAFSLVAEREGLLEHGV
                     LQATRRESGLLDFQGT"
BASE COUNT         1371 a         3524 c         3493 g         1397 t
ORIGIN      
        1 tgcccttacg gtcgcgcgcc ttcgtccgcg tgcgggcgca gcaggcggta gatggtcgag
       61 cgcgcgatgc cgagccgggt ggcggcggcg ctgacgttgc cgtcgagctt gtccaccacc
      121 cagcgcaggt aggcctgctc catctcggca agcgggacga tgcgctcgaa gccggtccgg
      181 ccagtgctcg cgaccgtcct ggcgccgggg tgcagcaagg catcgacgcg cgccgcgtcg
      241 atgcggtcgc cgtcggcgaa gacgagcagg cgctcgacca cgttgcgcag ctcgcgcacg
      301 ttgcccggcc aggcatggtc gatcaggcgc tgcagggcct ggggctcgac gcgcggggcg
      361 ggcgtgccgg ggtggcgccg caccgcctgg tgcagcaggt gctcgaccag cagcgggatg
      421 tcctcgcggc gctcgcgcag cggcggcacg gcgatgtgga cgacgtcgag ccggtacagc
      481 aggtcgtggc ggaagcggcc cgcggtcacg agcgcctcca ggtcgcggtg ggtggcggcg
      541 atcacgcgtg cgccggtgcg gcgcacggcg ctggcgccga gcgggcggaa ctcgccgctg
      601 tcgagtacgc gcagcagctt ggcctggcag gcgagcggca actcgccgat ctcgtcaagg
      661 aagagggtgc cggcgctcgc cagctcgaag aggccctcgc gatccgtgct cgcaccggtg
      721 aaggcgccgc gcttgtggcc gaagagctcg ctctcgacca gctcgccttc gagcaggccg
      781 cagttcaccg ggacgtaggc ctcgcccgcg cgccggctcc agcggtgcag cgcggcggcc
      841 accacctcct tgccggcgcc gctctcgccg gtgagcagca ccggcacgtc gagcgccgct
      901 gcgcggcgca cctgctcgag catccgcagc cacgccgggc tgtccccgac catgccgtcg
      961 tcgtcgcggc tgcgcaggcg cgccacctcg accgagagcg cgtcgcagcg gcgcgcgagc
     1021 gcgctgcgtt cgagcgcgcg cgcgaccacg aggtcgagct cggtgagctg gtagggcttg
     1081 gtgaggtagt cgaaggcgcc cagcttcatc gccgcgatcg cgctgtcgat gctgccgtgg
     1141 ccggtcagca ccacgacctc ggtgcgcggc gcgagctgct tgatgcgctc gagcagctcg
     1201 atgccgtcca tgcccggcat cttgatgtcg atgagggcga gctcgacacc gccgccctcg
     1261 atgcagcgca gcgcgccctc gccgtcgccg gcctcgctga tgcggtagcc ctggcggccg
     1321 aggcggccgc cgagcagctc gcggtagagc tcctcgtcgt cgacgatgag gacgtggcga
     1381 tcggtgatgg gtcgatcagt catgcttcgc ggctcccggt ccgtcttccg caagcggcag
     1441 caccaggcgg aaccaggccc cgccctgggg gcggttgccg caggcgatgc ggccacccag
     1501 ctcgttgatg atcgcgtagc agatcgacag gcccagcccg gtgcccttgc ccggtggctt
     1561 ggtggtgaag aagggatcga acacgcgttc ggcgaccccc ggcgcgaaac ccggcccgct
     1621 gtcgccgatc tcgatctcga tgccttcgcc gacggcgcgt gcggcgatgt cgatgcgccc
     1681 gctgccgttc atcgcgtcgc aggcgttctt gatcaggttc tgcagcacct gttcgagcag
     1741 ggccgggctg gtgccggcct gaggcagacc cggtgcgatg tcgaccgcga gctcgacgcc
     1801 gcgggtgagg ccggcgtcgt tgaagaaggc caggcgttcg gcgacgaaga ggcccacgtc
     1861 caccgtctcc aggctggcgc tctggcgccg cgagaaggcg agcaggttgg cggtgatcgc
     1921 cttgcagcgg tgggcctgct gctggatggt ggccagtccg cggcgcagct gcgcgagccg
     1981 ccggtcatca ccgagcggcg tgcattcgtc gagcagggcc tggagttcct cggcatagcc
     2041 ggacaccagg ccgagcgggt tgttgatctc gtgcgccacg cccgaggcga actggccgag
     2101 cgcgctcagg cggttggcct gcgcgagctc gcgctccatc tgccggcgcg cggtgatgtc
     2161 gcgcgccacg ccgatcacac cctcggcgac gtcgcggccg acgaagaggg tggagttgac
     2221 ctccagcacc acgctgctgc cctcgcgtcc ggagagcgcg atctcgaagg gccggccggc
     2281 ctcgccggcg atgcggcggg caaaatcgcg ctcgatgcgt gcggccgaag aggccggcat
     2341 gaagtcgccg acggcgtgcc cgatcgccgc ctcgggtgcg gcaccgagca gctcggccat
     2401 gcgggtgttg acgaagctca gccggccctg gccgtcgagg atatagaccg cctcgttgat
     2461 cacgtcctgg atctgctgct cgcgcgactc gatgcggcgc gcgtactcgg tgagctcgcg
     2521 ctcgcgttcg agctgggcgc tgatgtcgag cgccagcccg ctgtagccca cgatgcgatc
     2581 gtccttgagc acgggctcga cctgcagccg taccgggaag ggcaggcctg cggcgtcgcg
     2641 catcaccagg tcgagcgtgg tggtgctgcg gcgtgccgcg atctccttga ggaaggcggt
     2701 ggcgcgcgcg gtgtagtcgg gcagcacgag gtccttcagg cgcagcgcgt gcagccgcgc
     2761 ctcgcttacc ccgaggcgca ggcgcgccgc cttgttcagg tgcagcaggc gccccgaggc
     2821 gtgcagcgcg aacgccatct cggggaggtc ctcgatgagg ttgcggtagc gttgctcgga
     2881 ggcctcgagc tcgcgggtgc gctcatgcac ccggctctcg agcaggttga ggcggtcgcg
     2941 caggttgagc ggcttgaagt attcgcgcag gcccgcgagc tcgggcgcgc agtcgctggc
     3001 gttcatcagc gtccacgcgc agtggggatc gccgcgcccc tcgcaggccg tctccacgca
     3061 gatcatgtcg gcctgcagca cccgcgaggc gaagcccgag gcgtagccgg tcagcgtcca
     3121 gcacaccggc tcgtcggcgc gaccgaacag gcgcaggtgc tgctcggcct cgtaggagtg
     3181 ccgccagcgc ccgctcatgc ggtagcgccc gctggcatgg tcgagctcga gcgcatcggg
     3241 cgcgacctcg gcgatgcccg agaacatgtg ggtgcgcggt ccgccgagga tgaactcctc
     3301 caggctgtcc ggatgcatgt acgcggcaag cagctcggca tcctcgtagc cgcagctgaa
     3361 gccgtagcgc gtgagcacca ccttggcgat ctcgctgccc agggtctcga ccagctcctt
     3421 gcgcagcgcg cccatcgagt cggcatggac gaggatcgcc cgccgcccgc aaaagcggat
     3481 cgcgccgctg tcgggcatga agtcgatcag cgcgtggaag tcgagctcgt ccgaacgcat
     3541 cgccggcagg atctccgtgc ggccagggaa gaaacccggc ggcggccgag gccgtccccc
     3601 ggggaccgcg cagtctcgcg caattcgtcc tccccgtcca tgccgcgacg aacgcggttg
     3661 cgcggctgcg ccgggcggcg gcgtctcgcg ccgcatgcgc cggggcgtgc gctggctcac
     3721 ggcgcagatc ggcgagagcg gctaccctga tgcctttacg cataccctgc gtgcatggat
     3781 cccgcgatga tcgacccagg actgccggcc gacgagcccg agcgcctcgc cgaactgcgt
     3841 gcgctggcga tactcgacac gccccgcgag gacgccttcg acaacctgat gcaactggcc
     3901 gcgtcgatct tcggcgtgcc gatcgcgctc gtctcgctcg tggacgaggc gcgccagtgg
     3961 ttcaaatcgg cgcacgggct gtgcgtggcg cagactccgc gcgacgtctc cttctgcacc
     4021 catgtggtgt tctccaacgc gcccctggtg gtcgaggacg ccgcgcgcga cccgcgcttc
     4081 gccgacaacc cgctcgtcac cgctgcgccg caggtgcgct tctacgccgg cgagcccctg
     4141 cgttcggacc aggggttcgt gctcggcacc ctgtgcgtca tcgacaccgt gccgcgccgc
     4201 ttcgaggagg ccgaacgcgc acgcctgcgg ctgctcgcgc gccaggccga gcagttgatc
     4261 cgcctgcacc gcaagcgcca gcgcctggcc gacgaggcgc gccgcagcgc gcgcatcgcg
     4321 gcgcgctacc aggccatcac cgagggcgcg gccgccggca tcctgcgcat cgacgggcgc
     4381 gggcggatca tcgaggtcaa ccgcttcgcc tgcgcgctgt tcggctacga agaggcggag
     4441 ctgcgcgggc acaacgtgcg catgctgatg cccgagccct tccgttccgc gcacgacggt
     4501 catctcgagg cctatcagcg caccggcgtg gcgcgggtga tcggcaaggg caccgaggtc
     4561 gacgcgctgc accgcgacgg ccgccacatc ccggtgcagc tcaccgtcag cgaggtgagc
     4621 cagggcgcgc aggcggacga tttcgagggc cgccacttca tcggcatcct gtccgacctg
     4681 cgcgacgtgc atcaggtgcg cgagcgcgag cgccaggagc gcgcgctgct cgaggtgctg
     4741 caccgcggcc tcaccgacta ccacgcgctg atctcgggca atacgctgtg gggcttcctg
     4801 aaggacgcgc tgtgcgcgct caccggcagc gcgtatgggt tgatcggcga ggtggagcac
     4861 ggcgagggcg gcccggcgct caagatccac gccatcaccg acctgtcctg gaacgacgag
     4921 acgcgtgggc tgatgcgcaa gctggtgagc ggcgacatgc gcctgagcaa cccggcgagc
     4981 atgctcgggc gcgtcttcgc ccgcggcgag gtggtgctct ccaacgccat gcggtccgat
     5041 ccgcgcggcg gcgcgatgcc gcccgggcat ccgccgctgt cgcgctacct cggcgtgccg
     5101 atcatggacc gcggcgaggt catcggcatg ttcgcaatcg ccaacgcgcc gcaggactat
     5161 ccggacgagc tggtcgactg gctcaagccc ttcacctcga cctgcgcact gctgatcaac
     5221 ctgtaccgcc tgttcaagga gcagcagcgc ttcaccgaag agctgcagca ggcgcgcgac
     5281 caggccgaga aggcgaacca ggccaagacc gagttcctgt cctcgatgag ccacgagttg
     5341 cgcacgccgc tcaacgccat cctcggcttc gcccagctgc tgcagaacgg ccgccagccg
     5401 ctgtccgagc gccagctccg ccaggtcgag cagatcgccc gcagtggcag gcacctgctg
     5461 cagctgatca acgaggtgct cgacctcgcc cgcatcgagt cgggcaggct gcaggtctcg
     5521 ctcgaaccca tgctcgcgca cgacgtgatc gaggacgccg cccagatcgt cctcccgctc
     5581 gcggagcagc agggcatcgc gctggcgctg ccgccggcgc aggcctgcgc gctgcgcttc
     5641 acgggcgact acaccagggt gaagcagatc ctcatcaacc tgcttggcaa cgcgatcaag
     5701 tacaaccgcc ccggcggcgc ggtcgaggtg cgctgcgagg cggtcggcgg gcgctgccgg
     5761 atcggcgtcg tcgataccgg catcggcatc ccggcgagcc ggctgggcga actcttccag
     5821 cccttcaacc gcctcggcgc cgaatccggc agcatcgagg gcaccggcgt cgggctcgcg
     5881 ctcacccgca agctggtgga tctgatgcac ggcgagctcg gcgtgcacag ccgcgagggc
     5941 gagggcagcg cgttctggtt cgagctgccc ttgtgcgaac aggtgccggc ggtccgcgac
     6001 gaggcgcgtg cagcggccgc gccgcaagcc ctgagccggc gccaccgggt gctccacgtc
     6061 gaggacaatc ccgccaaccg ccagctgatg caggacgtct tcgaggacgt cggccaggcc
     6121 gtgctgcaca gcgtggattc ggccgaggcc ggcatcgagg cggcctgctc cgatccgccc
     6181 gacctcatcc tcatggacat cgacctgccc ggcatgagcg gtctggaggc gcgcgccctg
     6241 ctcgcgcgca acccgctcac cgcacacatc ccggtgctgg cgatctccgc cgcggcctcg
     6301 ccgcacaccg tgcgcaaggc gcgcgaggcg ggcttcatcg actacgtgac caaacccttc
     6361 gacgttcctg ccctcgtcgc gcgcctcggg cagatcctcg aacacaggag cagcgatgac
     6421 tgatcgcgtc cccatgcagg ccgacgccga catcctgatc gtcgacgaca accccgccaa
     6481 cgtcgagctg ctgctcgccc tgctcgaaga cgcgggctac ctgcgcctcg aaggcatcac
     6541 cgacccgcgc agggtggcgg cgcgggtggc ggagcacgcg cccgacctca tcctgctcga
     6601 tgtgcgcatg ccgcacctga acggcctcga gctcatggag cggctgtcgg cgggcggcgc
     6661 ggaggcgccg gcggtcatca tcctcaccgc gcagatcgac gatgccaccc gttaccgcgc
     6721 gctcgaactc ggcgcgcgcg atttcctcac caagcccttc gaccaggtcg aggtgttgca
     6781 gcgcatccgc aacgtcctgc acatgcagcg cctgatgaag gagcgcgccg aacgcgcgga
     6841 actgctcgaa gcgctggtcg ccgaacgcac gcaggaactc gtcgcgcgcg cgcgccacga
     6901 tccgctcacc ggcctgccca accggctgtc gctgctcgac gagctcggtg cacggctggc
     6961 ggcgcggcgt gacgccgcgg tgctcttcct gtcgatcgag ggcatggacg aggtttcgcg
     7021 cctgcacggc ttcggcgtgg ccgacgaact cgcgcgcgcg gcagcgcggc ggctgcgcga
     7081 atgcgttccg gagggcttcc tcgcggtgtg gaacagcacg cagtgggtcc tgctgtgcga
     7141 ctgtgctccg gcgaggggcg cgaccggcgc gctcgccgag cggctgctcg ccggcttccg
     7201 cccgccggtc gaggtcgaac acatgcggct gcacctcggt ctgcgcatcg gcatcgccgc
     7261 cgaggcggac gagcgcagcc cggagcagct cgtgcgcatg gcggcgctcg cgctgcccgc
     7321 aggcgagggg cagtggcagg cctacgaggc cgagctcgag acgcggatgc agcgccgcaa
     7381 cggcatccgc gaggccttgc gcggcgcggt cgggcgcgac gagttctcgc tggtgtacca
     7441 gcccaaggtg gagctgtgca gcggcgacat cgtcggcgtc gaggccctgc tgcgttggga
     7501 cagcagcgcc tacggacgcg tttcgcccgc cgagttcgtc cccatcgccg aggccagcgg
     7561 cgagatcctc gcgatcggcg gctgggtcgt gcgcgccgcg ctcgccacgc tgcggcgctg
     7621 gcgccaggcg gggcgcgtcg ccgacgtgtt cggcatggcg atcaacgtat cgacccagca
     7681 gctcatgcag ccagacttcg ccgagcgcct cgtcgccgag gtcgaggcca gcggcgtgcc
     7741 gcccgcggcg ctcgagctcg aggtcaccga atccggcctg atgcaggaca tggcgctcgc
     7801 cgcccgccac ctcgaccacc tcgcgcgcgc gggcttccgc atcgcgatcg acgacttcgg
     7861 caccgggcat tcctcgctcg cctacctcaa ggccttgccg gtctcggtgc tcaagatcga
     7921 ccgtgccttc attcgcgagc tccacgccaa tcccgccgac cagcgcctta ccggcaccgt
     7981 gatcgacatg gcgcggcatt tcgaattcat cacggttgcc gagggcgtgg agcaggaggc
     8041 ccagctcgaa cgcctcatgg acatgggctg cgacctcgtc cagggcttca tgttcgcccc
     8101 gcccctgaag gaggacgcgc tgttccggct gatgaagacg ggcttcgcgc acatgccggc
     8161 gttcggcggg gcggtggcgt agaggggtag gcgctggccc gacggcccgg ttcgcgactt
     8221 gcgtcgctcc cacggaaacc gccgatgccc cgatcgcgac ttgcgccgct cccacggaaa
     8281 ccgccgatgc cccgatcgcg acttgcgtcg ctcccacgga aaccgccgat gccccgatcg
     8341 cgacttgcgt cgctcccacg gaaaccgccc cgccccgtgg gagcggcgca agccgcgaaa
     8401 ggagctcagg gcaaccgcca gcccccgtag gagcggcgca agccgcgaat gaagcggttc
     8461 gggatgcgac ccgcgtgcag aacgccgccg ttcatcctcg cgcgttctcc gcttcgtctt
     8521 ccccgtcacc gcctccacgc ccgcgccggc gatcgtctac gttccctgaa agtccagcag
     8581 cccgctctcg cgccgggtcg cctgcaacac gccatgctcc agcaggccct cgcgttcggc
     8641 caccaggctg aaggccttgc gggcgcgcgt gatcgcggtg tagatcagct cgcgcacgag
     8701 cacgttgccg ctctgcgcgg cgagcaccag caccgtgtgc tcgaactccg agccctggct
     8761 cttgtgcacc gtcatcgcga acgcggtctc gacatgggcg aggcggctcg ccgccaccga
     8821 gcgcaaggcc tcgccgtcga ggaagtgcac ccgcaggccg ccgctctcga gcgccggcag
     8881 ggtgatgccg atgtcgccgt tgaagacgcc gagcgcgggg tcgttgcggg tgaccatgac
     8941 cgggcgcccg gcgtaccagg tcccgcgtgg cgacagcaga cgcgcgcgcg cgagcgcgtg
     9001 ctcgatcgcg cggttgagcc cggccatgcc ccagtcgcct tcgcgcaccg cgcacagcac
     9061 gcggaagcgg tcgaaggcgt gcaggatcgc cgtcgcccac gcggcgtgcg cctcggcgtc
     9121 cttgcctgcc ggtccgcgag cgagcagctc cagatagtcg cggtagcagg cgggtgcgcc
     9181 cctgcggccg ccgagcgcga gcgcgatcac cgccgccacc ggtccgccct cgaccccgtg
     9241 cagcgcgccg ctcgcatcct cgcgcagcag gcgcgcgggc agggccgggt cgcgcgccgc
     9301 gttgaccgcg tgcgcgagcg cgccgatcgg cccctcgaag cgccggctct cgcgcagcat
     9361 cacggtgtgc tgggcgagcg cgggcgcctt gtccgtgggc tccaggtagc gcgtgggcag
     9421 ggcctcgccg gtggtggcga gcaggtagcg tgcggtctcg gcgtcgtagc gtccgtccga
     9481 ggcgtcgcgg cagaggtcgc cgagcaccgc gccggcctcg accgacgcga gctggtcctt
     9541 gtcgccgagc agcaccaggc gcgcgccgtg cggcagggct tcgagcaggg cggccatcat
     9601 ctccaggtgc accatcgagg cttcgtccac gatcaccacg tccacgtcga gcggattgcc
     9661 ggcgtggtgg cggaagcgcc gcgtgtcggg acgggcgccg agcagcgcgt gcagggtgcg
     9721 tgcggcgccg atgcggcggg tcagcgcggc gaggtcgagg tggccgccca ggctgcgctg
     9781 acccg
//
LOCUS       DAOQNN010000079         9781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3300_length_9781_cov_9.072486, whole genome shotgun sequence.
ACCESSION   DAOQNN010000079 DAOQNN010000000
VERSION     DAOQNN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3300_length_9781_cov_9.072486"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PKV97_06330"
     CDS             complement(<1..402)
                     /locus_tag="PKV97_06330"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNB05532.1"
                     /translation="MNPASGPHGLPPLEQIRRRLAEPRREGCFNGDGRDLARSRPPKP
                     AAVLVPLVVRGEAPSVLLTRRTDHLHHHPGEISFPGGRLEDDDPSPVHAALRETEGEI
                     GLARRHVELIGALPDYFTGTGFRVTPVVGLVH"
     gene            complement(411..1061)
                     /locus_tag="PKV97_06335"
     CDS             complement(411..1061)
                     /locus_tag="PKV97_06335"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HNB05533.1"
                     /translation="MDGWKAASVGVLAIAAGLGAGAAYAADVAVAGLFAGRAVLVVDG
                     GAPQTVKVGARTREGIVLRAATSEAATLEHDGRVFVARLGEQVASSAGTAASEISLQA
                     DGGGHFFTGARINGVAVNALVDTGATLVAMGRSDALRLGIDYRAGTMGQSATANGLAT
                     IWKVRLDAVELGGVRMLGVDAAVHETDLPVVLLGMSFLNRMDWRREGDRLLLKKRY"
     gene            1286..1714
                     /locus_tag="PKV97_06340"
     CDS             1286..1714
                     /locus_tag="PKV97_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HNB05534.1"
                     /translation="MKHASRTRGFTLIELMIVVAIIGILASIALPAYSDYIRRSKIVE
                     ATSVLSDLRVRMEQFYQDNRSYVSTGTTCGVAMPTQQFFTMSCTGTSTTYTLTATGKS
                     GTDVAGFTYTLNQQNVRATTGGSFGTSTTCWVGKRGGGCL"
     gene            1711..2247
                     /locus_tag="PKV97_06345"
     CDS             1711..2247
                     /locus_tag="PKV97_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNB05535.1"
                     /translation="MRTPIQRGFGLVEAMVTIAVLAILLAVAVPSFQQMIVNGRTRTI
                     AESFRAGVDLARTEAMRRNLPVGFAVVSAVTGCSADTTGTNWVVFLGTTCNNDATVVQ
                     RFVAGGSEAGVTVSTSRGTLIFNGIGMSVGGATATIDFGNNLPSAQRRTLRLGVSAGG
                     QTRLCEPGITASGDPRAC"
     gene            2259..2741
                     /locus_tag="PKV97_06350"
     CDS             2259..2741
                     /locus_tag="PKV97_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNB05536.1"
                     /translation="MKRTVRLPIQRGRSKACAGSRPQRGASLLEVLIALLIFSFGVLG
                     IVGLQASMNIRSTEARQRAEAALLADEIIGRMWLAPAIRREDFVYRSDADCTATATTP
                     PDILSSWLNKLRATIPGAQVVIGHDETTGETRLTICWPRPQTNEWSNHVVRARINSNN
                     "
     gene            2842..3876
                     /locus_tag="PKV97_06355"
     CDS             2842..3876
                     /locus_tag="PKV97_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HNB05537.1"
                     /translation="MTIGLIALLVMVQTFSASNAHKANTVSGADATTAGQVAMTLLER
                     DLLNAGAGLIGTGCRTIKYLKPGTSGAQEMNGMPVTIVADPSAPSALASRSDRITIRY
                     ASSGEASIAKATLTESMPSPSSTMFANIGMGFSEDDLIVLNDAEGHCAIQQLTQDPGS
                     VGRGGGAWRFQINPSSPYNDPGDSAGLFPSDGYQSGTGTIRSLGPGGIAETVYRLQSR
                     GSSTAPPDTDLATSRADMASTTAAQTLVRDVVALRAQYGWWNRTTNTVSFSSTIPTGA
                     GAADLVAVRVGILVRASQRDASYTAPETFSFFEYDTPVQITLGSAERNYRYRSYETVV
                     PLRNALWNRE"
     gene            3861..4502
                     /locus_tag="PKV97_06360"
     CDS             3861..4502
                     /locus_tag="PKV97_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HNB05538.1"
                     /translation="MEPRVMHGERRQGGAALFMALIMLVAMSIAAVSLVRSVDTTVTI
                     AGNLAFQQAALQASDFGVEAAAHDLDTKPRESEWKVGGTCRLTSAEPTVSNYFPTMFA
                     TASTMGSPTIGGRSYTIPGVPTLDWDKVACIEDSRIPDGYRVQYVIDRMCTGTLPIAN
                     RAESCVSDNPEEGGSRRAGSPRITGASSIRYRLTVRVTGPNNAQSFVQVVLRD"
     gene            4502..>4839
                     /locus_tag="PKV97_06365"
     CDS             4502..>4839
                     /locus_tag="PKV97_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05539.1"
                     /translation="MDSSPLTGERVVNNAHPAFRPLARHTRLALLAAGLLAAQIGFAA
                     QTEISEVPPESKITVPANVMFTLDDSGSMYWESIPDSHGFRDGIYLFPRPANVYDWRT
                     GATDYPANPVS"
     assembly_gap    4840..4939
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4940..7876
                     /locus_tag="PKV97_06370"
     CDS             <4940..7876
                     /locus_tag="PKV97_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HNB05540.1"
                     /translation="GNFTRVQIGLTTLPTRSPKRTDCAGDPCSYAEEIKNFANWFSYH
                     RSRHLAARAAIGKAFSEQKDNLRVGYATINRTTETDIDGFTTKRVVRGLRLFKDEEAS
                     DKRWRTQFFDWLYKDPMPRLGTPLRTAMDDVGNYFTSAPPWRAKVEDSTSAALSCQRS
                     HHILMTDGYYDNDGDSARASISGSNVDNLEGDEHTSEDGTRSFSYLPAAPYKDDYTNT
                     LADVAMYYWKTDLRPGLANGIPDDDRNPAFWQHLSTYTIGFGITGMLSESDIVALFAG
                     RLNSVSWLNPTTVGNDRAKGDDLVHAALNGRGEFFRADDPELFAQRMSKVLESLANNP
                     SGAAAAAVTKPYIDIANNFTYETSYRAAQRMGDIKAYKLHLETGQPDRNQPAWTKPCP
                     TDASRTCAKGVAEMLEARTPDSRQIATFNGSSGVDFTSSGLGTTLLGQLQSPAGAGNG
                     AQVLAYLRGDRSQESTSLRRRLQLLGPIIHSEPVLVREPDRSYADAGYASFRSSTTGR
                     TRMLYVGANDGMMHAFDASTGAEKWAYVPKAVFPRLPGLASPNGLFEATVDGYLTEGD
                     VDFGFTGGTRGSADWRTLLVGGLGRGGKGFYALDITSPEVTESSPLTAKVLWEFSGDA
                     DLGLAFSRPIIVKHEVVTTDNRGNETRSGQGWVVLVSSGYNNSSGDGRGYLYVLNAKT
                     GALIKKLSTGVGSASDPSGLGQLSAWVHNADVNNGVQWVYGGDLKGNLWRFDLSATNP
                     DAWSTSKLAVLTDASGNVQPITAAPELTRVKAGNDFRNLVVVGTGRYLGEKDVPGTTV
                     SAADAAQVQTFYVIEDRIDRGGSTVVRADLTPRTLAAETDGTRTLSDAADIDWSAKRG
                     WRVDLNISGERATTDPAIAHTTVAFTTNVPSTSDPCVPGGTSYLFQFDLATGMAPIGT
                     NALPYVGKRLRPGLASRVVLIVTPNGQVIGLIRKTDDSSEAEPIVTSVSGGDIRRISW
                     RELNYR"
     gene            complement(7949..9043)
                     /locus_tag="PKV97_06375"
     CDS             complement(7949..9043)
                     /locus_tag="PKV97_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB05541.1"
                     /translation="MNPQPRLMALVLSMIGMSAVQAAPIISVRTYDIAERSYAVTDSS
                     VMDGAVSTAITTSGSVGPNSFQQFDASTGVLTSITASVSASNLLLSQQVTGGATGTSA
                     VRVSWTSFGESLFDRQVIGSVSTPTPSLSNFDTLSRTIASGDAAGDAFVAKAGQPPGN
                     SAASYELVADLSSTAPGSADSLTSSLSSRTADAAGSFTHKLAYSYVPHAIASFEGDQT
                     TTTWRLPVSGNIEVELGDLFTLDLNIFNLGLLGGAGLDLDGFNPKSGSGLSMTDLELF
                     EGLAQGTGRNVKASFRADELGSHEMMFSLLLSDEDVGFASSRGSYELELTVFANVVDN
                     GNGNPVPEPGVLALLGIGLFGLAVRGRAAR"
     gene            complement(9297..9494)
                     /locus_tag="PKV97_06380"
     CDS             complement(9297..9494)
                     /locus_tag="PKV97_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HNB05542.1"
                     /translation="MEATAIAALASNNALAQVQQEASVEVLRKALDLQGESAMQLLQA
                     IPRPPAAAVGSTAGGIVDTWA"
BASE COUNT         1767 a         3462 c         2993 g         1459 t
ORIGIN      
        1 atgcaccagc cccaccaccg gcgtcacccg gaagccggtc ccggtgaaat agtcgggcag
       61 cgcgccgatc agttcgacgt gacggcgggc gagcccgatc tccccttcag tctcgcgcag
      121 cgcggcgtgc accggcgaag ggtcgtcgtc ctcgaggcgg ccgccgggaa agctgatctc
      181 gcccgggtgg tgatgcagat ggtcggtgcg ccgggtgagc agcaccgacg gcgcctcgcc
      241 gcgcaccacc agcggcacca gcaccgccgc cggcttgggc ggacgcgagc gcgcgaggtc
      301 gcgaccgtcg ccgttgaagc agccttcccg gcgcggctcg gccagccggc ggcggatctg
      361 ttccagaggg ggaagaccat gcggtccgct agcaggattc acgcgacgcc tcagtaacgt
      421 ttcttgagca gcaggcggtc gccctcgcgc cgccagtcca ttcgattgag gaaactcatg
      481 ccgagcagca ccacgggcag gtctgtctcg tgcaccgccg catcgacccc gagcatgcgc
      541 acgccgccga gctccaccgc gtccagcctc accttccaga tcgtcgccag cccgttcgcg
      601 gtcgcgctct ggcccatcgt gccggcgcgg tagtcgatgc caagccgcag cgcatcgctg
      661 cgccccatcg ccaccagggt ggcgccggtg tcgaccaggg cattgaccgc cacgccgttg
      721 atgcgcgcgc cggtaaagaa gtgcccgccc ccgtcggcct gcagactgat ctcgctcgcc
      781 gcggttcccg cgctgctcgc cacctgctcg cccaggcgcg cgacgaagac acggccgtcg
      841 tgctccagcg tggccgcctc gctcgtcgcc gcccgcagca cgatgccctc gcgcgtgcgt
      901 gcgcccacct tcacggtctg cggcgcgccg ccatcgacca ccagcaccgc ccggcccgcg
      961 aacagcccgg ccaccgccac gtcggcagcg tacgccgccc ccgcgcccaa gcccgccgcg
     1021 atggccagca cgccgaccga agccgccttc catccgtcca tcccgtcctt cctccgcatc
     1081 gcgccgacct ccctccccgt tgccgatccg ccaagcgggc ccgcccgcgc tgcgcaccgc
     1141 ccgccgatca tcctccgagc ccccgccgtt cgtccacccg cgacggcggc attctgcgtg
     1201 gcgccataga atccgtcacg aacaccccgg cggcaaagtc cgacaatgca atcgagactg
     1261 tgcaccatcc gacacggagc cccccttgaa acacgcctcc cgcacccgcg gcttcacgct
     1321 gatcgaactg atgatcgtgg tcgccatcat cggcatcctc gcctcgatcg ccctgcccgc
     1381 ctactccgac tacatccgcc gctcgaagat cgtcgaggcg acctcggtgc tgtccgacct
     1441 gcgcgtgcgc atggagcagt tctaccagga caaccgcagc tacgtgtcga ccggcaccac
     1501 ctgcggcgtc gccatgccca cacagcagtt cttcacgatg agttgcaccg gcaccagcac
     1561 cacctacacg ctgaccgcga ccggcaagtc cggcacggac gtggcagggt tcacgtacac
     1621 gctgaaccag caaaacgtcc gcgcgacaac cggcggcagc ttcggcacca gcacgacctg
     1681 ctgggtcggc aagaggggag gcggctgtct atgaggacgc cgatccagcg cggtttcggc
     1741 ctggtcgagg ccatggtgac gatcgcagtc ttagcgatcc tgctcgcggt cgccgtaccc
     1801 agcttccagc agatgatcgt caacggccgc acccgcacca tcgccgaatc cttccgcgcc
     1861 ggtgtggatc tggcccgtac cgaggcgatg cggcgcaatc tgccggtcgg gttcgccgtc
     1921 gtctccgccg tcaccggctg cagcgccgac accaccggca cgaactgggt ggtcttcttg
     1981 ggcacgacct gcaacaacga cgcgacggtg gtgcaaaggt tcgtcgccgg gggtagtgag
     2041 gcgggcgtca cggtcagcac ctcgaggggc acgctcatct tcaacgggat cggcatgagt
     2101 gtgggcggag ccacggccac catcgacttc ggcaacaatc tcccctccgc acaacgacgt
     2161 acgctccgcc tgggcgtcag cgcgggcggc cagacccgcc tgtgcgagcc cggcatcacc
     2221 gccagcggcg acccgcgtgc ctgttgagga ggccagccat gaagcgcacc gtgagactcc
     2281 ccatccagcg cggccgctcc aaggcctgcg cgggcagccg tccgcagcgc ggcgccagcc
     2341 tgctcgaggt gctgatcgcc ctgctgatct tctccttcgg cgtgctcggc atcgtcggcc
     2401 tgcaagccag catgaacatc cgcagcaccg aggcccggca gcgcgccgag gcagcgctgc
     2461 tggccgacga gatcatcggt cgcatgtggc tggcgcctgc gatcaggcgt gaggattttg
     2521 tctatcggtc cgatgccgac tgcactgcga ccgcgacgac accgccagac atcctgtcgt
     2581 cctggttgaa caagctgcgg gcgaccatcc ccggcgccca ggtggtcatc ggccacgacg
     2641 agaccactgg cgagacccga ctcaccatct gctggccgcg cccgcagacc aacgaatgga
     2701 gcaaccatgt cgtccgcgcc cgcatcaatt cgaacaactg aagccgcgcg gccggcaccc
     2761 gggcgcgcaa gcgccggcag atggcgccgc gctgcgaggc tacgtacgcg cggcattggc
     2821 ctggtcgagt tgatggtcgg catgaccatc ggcctgatcg cgctgctcgt catggtgcaa
     2881 accttctcgg caagcaatgc acacaaggcc aacacggtca gcggcgccga cgcgacgacg
     2941 gccggccagg tggccatgac gctgctggaa cgcgatctgc tgaacgcggg cgccggcttg
     3001 atcggcacgg gctgcagaac gatcaagtat ctcaagcccg gcaccagcgg agcacaggag
     3061 atgaatggca tgccggtcac gatcgtggcg gatccgagcg cgccgagcgc cctggcctcg
     3121 cgcagcgatc gcatcacgat ccgctacgcg agttcgggag aggcctcgat cgccaaggcc
     3181 accctcaccg aatccatgcc cagcccctcg tccacaatgt tcgcgaacat cgggatgggg
     3241 ttttccgagg acgatctgat cgtgctcaac gacgcggaag gccactgcgc gatccagcag
     3301 ctcacccagg accccggctc ggtcgggcgc ggaggcggtg catggcggtt ccagatcaac
     3361 ccgagcagtc cgtataacga tccgggcgac tcggccggcc tgttcccctc cgacggttac
     3421 cagtcgggga ccggcacgat ccgctcgctc ggcccgggcg gcatcgccga gacggtctat
     3481 cgcctgcagt cgcgcggcag cagcaccgcg ccacccgaca ccgacctggc aacctcgcgc
     3541 gccgacatgg cgagcaccac ggccgcgcag acgctcgtgc gcgacgtcgt cgccctgcgg
     3601 gcccagtacg gctggtggaa caggacgacc aacaccgtga gcttcagttc gacgatcccc
     3661 accggagccg gggcggcgga cctggtcgcc gtgcgcgtgg gcattctggt gcgcgcgagc
     3721 cagcgcgacg ccagctacac cgcgcccgag accttcagct tcttcgaata cgacacgccg
     3781 gtccagatca ccctgggcag cgcggagcgc aactaccgtt atcggagcta cgaaaccgtg
     3841 gttccgttga ggaatgcctt atggaaccgc gagtgatgca cggtgaacgc cgccagggcg
     3901 gcgccgcgct tttcatggcg ctgatcatgc tcgtggcgat gagcatcgcc gcggtctcgc
     3961 tcgtgcgcag cgtagacacc acggtcacga tcgccggcaa cctcgccttc cagcaggcgg
     4021 cactgcaggc ctccgacttc ggcgtcgaag ccgccgcgca cgacctcgac accaagccgc
     4081 gcgagagcga gtggaaggtc ggtgggacct gtcggctgac gagcgccgaa cccacggtct
     4141 cgaactattt cccgaccatg ttcgccacgg cgagcacgat gggcagcccg accatcggtg
     4201 ggcgcagcta cacgattccg ggggtcccca cgctcgactg ggacaaggtc gcctgcatcg
     4261 aagactcccg gatccccgat ggataccggg tgcagtacgt gatcgaccgc atgtgcaccg
     4321 gcacgctgcc gatcgccaac cgcgccgaat cctgcgtttc ggacaacccc gaggaaggcg
     4381 gatcccgccg tgccggttcg ccacgcatca ccggcgcctc gtcgatccgt taccggctga
     4441 ccgtccgcgt caccggcccg aacaatgcgc agtccttcgt gcaggtcgtg ctgcgcgact
     4501 gatggactcc tcgcccttaa ccggagaacg cgtcgtgaac aacgcacacc ccgccttccg
     4561 gccgcttgcc cggcacaccc gcctcgcgct gctcgcagcc ggcctgctcg ccgcccagat
     4621 tggcttcgcc gcccagaccg agatttccga ggtccctccc gaatcgaaga tcacggtgcc
     4681 ggcgaacgtg atgttcacgc tcgacgactc cggctcgatg tactgggaga gcatccccga
     4741 ttcacatggt tttcgggacg gcatctatct ctttccgcgc ccggcgaacg tctacgattg
     4801 gcgcaccggc gccaccgact atccggccaa cccggtctcn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnng gcaacttcac ccgggtacag atcggcctca ccaccttacc
     4981 cacccgcagt cccaagcgta ccgactgtgc cggcgatccc tgcagctacg cggaggaaat
     5041 caagaacttc gccaactggt tctcctacca ccgctcccgc cacctcgcag cacgcgcggc
     5101 gatcggcaag gccttttccg aacagaagga caacctgcgt gtcggctacg cgacgatcaa
     5161 ccgaaccacc gagacggaca tcgacggctt cacgaccaag cgggtcgtgc gcggcctgcg
     5221 gctgttcaag gacgaggagg cctccgacaa gcgctggcgc acccagttct tcgactggct
     5281 ctacaaggac ccgatgccgc gcttgggcac cccgctgcgc accgcgatgg acgatgtcgg
     5341 caactacttc acgagcgcgc cgccctggcg tgcgaaggtc gaggactcta ccagcgcggc
     5401 cttgagctgc cagcgcagcc accacatcct gatgaccgac ggctactacg acaacgacgg
     5461 agacagcgca cgcgcgtcca tctcgggatc gaacgtcgac aacctcgaag gcgacgagca
     5521 cacgagcgag gacggcacca ggagctttag ctacctgccg gccgccccgt acaaggacga
     5581 ctacaccaac accctcgccg acgtggcgat gtactactgg aagaccgacc tgcgcccggg
     5641 cctcgccaac ggcattcccg acgacgatcg gaacccggcc ttctggcagc atctctccac
     5701 ctacaccatc ggcttcggca tcaccggcat gctctcggaa agcgacatcg tcgcgctgtt
     5761 cgcgggccgc ctgaactcgg tgtcctggct gaacccgacc accgtcggca acgaccgggc
     5821 aaagggcgac gacctcgtcc acgcggcact caatggccgc ggagaattct tccgcgccga
     5881 cgatccggag ctcttcgccc agcgcatgag caaggtgctc gaatcgctcg ccaacaatcc
     5941 gagcggcgcc gcagccgcgg ccgtgaccaa gccctacatc gatatcgcga acaatttcac
     6001 ctacgagacc agctacaggg ctgcgcagcg gatgggcgac atcaaggcct acaagttgca
     6061 cctggaaacc gggcagcccg accgtaacca accggcctgg acaaagccct gcccgaccga
     6121 tgcgagcagg acctgcgcca aaggggtcgc cgagatgctc gaggcgcgca ccccggactc
     6181 gcgccagatc gccaccttca acggcagcag cggagtggac ttcaccagca gcggcctcgg
     6241 caccacgctc ctcggtcagc tccagtcccc ggcaggggct ggcaatggcg cacaggtgct
     6301 cgcctacctg cgcggcgacc gcagccagga gtcgacgagc ttgcgtcgcc ggctgcagct
     6361 cctcggtccg atcatccaca gcgaaccggt cctggtgcgc gagcccgacc gctcctatgc
     6421 cgacgccggc tacgcgagct tccgctcgag caccacgggg cgcacccgca tgctttacgt
     6481 cggcgccaac gacggcatga tgcatgcatt cgacgcctcc acgggcgcgg agaagtgggc
     6541 ctacgtcccg aaggccgtgt ttccgcggct gcccggactg gcctcgccca acggactgtt
     6601 cgaggccacg gttgacggct atctcacgga gggcgatgtg gatttcggct tcaccggggg
     6661 aacgcggggc tccgccgact ggcgcacctt gctggtgggt ggccttggcc gtggcggcaa
     6721 gggtttctat gcactcgaca tcacctcccc cgaagtgacc gagtccagcc cgctcacggc
     6781 gaaggtgctg tgggaattca gcggcgacgc cgatctcggc ctcgccttct cgcggccgat
     6841 catcgtcaag cacgaggtag tcacgaccga caaccggggt aacgagacgc gctcgggcca
     6901 ggggtgggtc gtgctcgtca gctcgggcta caacaactcg agtggcgacg gcagaggcta
     6961 cctgtatgtt ctcaacgcca agaccggtgc gctcatcaag aagctctcca cgggcgtcgg
     7021 cagcgccagc gacccgagcg ggctcggcca gctcagcgcc tgggtgcaca acgccgacgt
     7081 caataatggc gtgcagtggg tctacggtgg ggacctgaag gggaacctgt ggcgcttcga
     7141 cctgagcgca accaaccccg acgcctggtc gacaagcaaa cttgcggtcc tcaccgacgc
     7201 cagcggcaac gtccagccca tcaccgcggc gcccgaactc acccgcgtca aggccggcaa
     7261 cgatttccgc aacctggtcg tggtgggcac ggggcgctac cttggagaga aggacgtccc
     7321 cggtaccacg gtctccgcgg ccgacgcggc ccaggtccag acgttctacg tcatcgaaga
     7381 tcgcatcgac cgcggcggca gcaccgtcgt gcgcgcagac ctgacgcctc ggaccctcgc
     7441 cgcggagacc gacggcacgc gcaccctgtc cgatgcagcg gacatcgact ggtctgccaa
     7501 gcgcggctgg cgggtggatc tgaacatcag cggcgaacgc gccacgaccg atcccgcaat
     7561 cgcccacacc acggtcgcat tcacgaccaa cgtcccgagc acgtccgatc catgcgtgcc
     7621 gggcgggacg agctatctgt tccagttcga cctcgcaacc gggatggctc cgatcggtac
     7681 aaacgccctt ccctacgtgg gcaagcgcct gcggcccggt ctggcgagcc gcgtcgtact
     7741 gatcgtgacg ccgaacggcc aggtcatcgg cctgatccgc aagaccgacg acagcagcga
     7801 agccgagccg atcgtgacct cggtgtccgg gggcgacatt cgccggatat cctggcgcga
     7861 actgaactat cgctgagacc ggacacgatc actggaaacg agaaaggcgc cgacggcgcc
     7921 tttctcgttt cgggaaccac ctaagaagtc atcgcgcggc acgcccgcgc acggccagcc
     7981 cgaagagacc gataccgaga agcgccagca cgcccggctc gggcaccgga tttccgtttc
     8041 cgttgtcgac gacgttggca aacaccgtca gctccaactc gtagctgccg cgactggagg
     8101 cgaacccgac atcctcatcc gacagcaaga gcgagaacat catctcgtgg ctgccaagct
     8161 cgtccgccct gaagctcgcc ttgacattcc ggcctgtgcc ctgcgcgagc ccctcgaaca
     8221 attcgaggtc cgtcatcgac agcccggaac cgctcttggg attgaatcca tccagatcca
     8281 gtcccgctcc gccgagcaaa ccaaggttga agatgttcag atcgagcgta aataagtcgc
     8341 cgagctcgac ctcgatgttc cctgacacag ggagccgcca agtcgtcgtg gtctggtctc
     8401 cttcgaagga ggcgatcgca tgaggaacgt agctgtaagc gagcttatgg gtgaacgatc
     8461 ccgccgcgtc ggccgtacgg gagctgaggg atgaggtcaa agagtcggcc gaacccggcg
     8521 ccgtcgaact caggtcggca acaagctcgt acgaagccgc gctgtttccg ggcggctgcc
     8581 cggctttcgc gacgaaggcg tctccggccg catcacccga ggcgatggtt cgagaaagag
     8641 tatcgaaatt gctcagcgac ggcgtcggcg tgctcaccga accgatgacc tggcgatcga
     8701 acagggactc gccgaaggac gtccagctga cgcgcaccgc actcgtcccc gtcgcgccac
     8761 ccgtgacttg ctgcgagagc agcaagttcg aagcggagac cgatgcggta atcgaggtca
     8821 gcacgcccgt cgatgcatcg aactgctgga acgaattcgg gccgaccgag ccgctggtcg
     8881 ttattgccgt gctaaccgcg ccgtccatca cggacgagtc ggtcactgca taacttctct
     8941 cagcgatatc gtacgtccgc acggaaatta tcggtgcggc ttggacagcc gacatgccga
     9001 tcatggaaag caccagcgcc atcaggcggg gttgaggatt catgtggttc tcccgttcta
     9061 tttgagcaag cgggtattac atgcttgccg acgagcgtta ccagcaaaaa tcaagccaga
     9121 agaaattttg cttaataatc cgtgacttaa attattaccc ccccccgaac cctgggtaac
     9181 tgtaaaaaaa accgacgcaa agcgaaatcg aggcctcatc gatgccccaa aatggggtcc
     9241 agccaatggc tgcgagacgc atgggtggtt ttcgtggttc gggcccgacg acctgctcaa
     9301 gcccaggtgt cgacgatgcc gcccgccgtg ctgccgaccg ccgcagcggg gggccgggga
     9361 atcgcctgca gcagttgcat cgcgctctcg ccctggaggt cgagcgcctt gcgcaggact
     9421 tcgaccgagg cttcctgctg gacctgcgcg agcgcattgt tcgaggccag cgcggcgatt
     9481 gcggttgctt ccatgatttg ttctcctgtc gagccggggc gagggcggtg atgtcgaaga
     9541 gcatatcggc agggagcgga ggagcttgag ccgcaccggc ggcagcgggt gacggaccgc
     9601 ccgcatcgcg acttgcgccg ctcctgcagc gcgtgcaggt gtcggaaata cgcgaacgca
     9661 gcgcgctcgg catgcgtgag gaccgcccca cccgcgcggg aaacaacggc cgaaccccag
     9721 ccgaaccgcg tccgaaatcg cgctgcttct cgtgggagcg acgcaagtcg cgattccccg
     9781 c
//
LOCUS       DAOQNN010000080         9761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3311_length_9761_cov_10.105605, whole genome shotgun sequence.
ACCESSION   DAOQNN010000080 DAOQNN010000000
VERSION     DAOQNN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3311_length_9761_cov_10.105605"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PKV97_06385"
     CDS             <1..845
                     /locus_tag="PKV97_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB05543.1"
                     /translation="LVADAIRRSRAGLSDENRPYGSFLFLGPTGVGKTELCKTLANFL
                     FDSEEHLIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILFDEVE
                     KAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSQMIQAMAGDDYGVIKL
                     AVMAEVKTYFRPEFINRIDEVVVFHALDEKHIAGIAKIQLQYLEKRLARLEMGMEISD
                     AALAELATAGFDPVFGARPLKRAIQERIENPLAKAILEGRFAAKDCIRVDAEGGQLVF
                     RRAD"
     gene            complement(921..4202)
                     /locus_tag="PKV97_06390"
     CDS             complement(921..4202)
                     /locus_tag="PKV97_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB05544.1"
                     /translation="MRASPLPAVVLALAYWAVGYPALHLAIPPGYTAPFFPPAGIALA
                     ALFVFGLRLWPAVFAGSALVQLATAWPLLGSAGWNPLGPLAVPVGATLQAVVGVLLAR
                     RLLAPGDTLDTAGSVMRFLGICMPLSGLVCSTIGVSALVWAAVIPASDALFNWWTWWT
                     GDTLGMLVAAPLTLVFIGRPAQDWRQRRLGVALPMSIALVLLALAYRQVVSWEDSRVR
                     AQFERDSVHLSALLQKRLEEQVDMVRSIERLLLASDQVSRADFHEFVAPWMRLHPGTR
                     SFTWNPYVRHAERAAFEEGIRSDYRVDYRILDRDPAGQTSPATERREYFPVTHVEPEE
                     GNHAVLGMDPLSFPVAGEAIERALATGQPAASARMRLVQEHGEQHAVVLYQAVFASSP
                     PAPVRAPLGVVSAALGMDDLLAAVSRQAAAQGIAVRLSDRDAGPESALLAGDDRGQES
                     VWPEEILARTTSIDFAGRRWELHTRALPRYIEQLRSWAAWGTIAVGVLTSAMLGALLL
                     ITTGNRRRIQALVEHRTAELQAASVRLHENRAALIDAQRIARLGSWETLCARDGLHAS
                     DELHLLLDSTAGDLNSLADLIDAIAPASRAALTDALERAATEPGRIMLDCNTDSLPMR
                     TLQFRIEGEHVGGRLRRIRGTAQDVTALREAEARIRYLARFDPLTGLLNRSAWQDHAQ
                     DVLRHAARHDDRCAVLFLDLDDFKTVNDSLGHAAGDRLLAQVACRLGGCVRAEDLVAR
                     IGGDEFVILLSRLAHAEEPARVAERVLDALARPLQIDSQPLRVGASIGIALYPADGGE
                     IDTLLKHADTAMYEAKESGRNAYRFFLPEMTQRVTRRLQTEAELRKAIESGDLRLHYQ
                     PQIDAASGRVYGCEALVRWQHPERGLLPPDRFVPFAEQSGLIVPLGEWVLREACLQQV
                     RWRDAGLPPLDVAVNISALQFRRRDFVATVARVLGETGADPARIELEITESALMDATP
                     ELVASFDQLVALGLTLALDDFGTGYSSLSYLKRLPLRRLKIDRSFVDGLPDNPEDAAI
                     TSATLSLARDLGMEVVAEGVETEEQRGYLCKRGCRAMQGYLFSRPLGVEEFRTWFEEQ
                     AAAIA"
     gene            4354..6249
                     /locus_tag="PKV97_06395"
     CDS             4354..6249
                     /locus_tag="PKV97_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNB05545.1"
                     /translation="MFEAVRNNKRVAQVILALLIVPFAFFGMDAYFSDSGSANEAARV
                     GGTTIGAWEFDQALREQQDRLRQDGGGQVDRALLQSAELRRAVLENLINQRVLALYAA
                     ENRLVVTPQQLQETIAGVASFQENGSFSLQRYETLLRAQGMTPATFEARLAQDVRVQQ
                     IVAAVGDAGIVPQASARRFLDAQLESRSVREFRLSAKALAADVKVDDAQVSAYYEANP
                     TRFERPARLQAEYLVFDRAAVERGVEVADDAVRAFYDGNPQRFGVPEERQARHILLAL
                     DQGAEQAEVDRVMAEARALVEQLRADPARFAALAKEKSQDPGSAGNGGDLGFFARGVM
                     VGAFEDAVFGLQKGVIGEPVRSDFGVHIVEVTDIKPSSVKPFEAVRAEILAELRAQEA
                     GRRFAELAEQFSNTVYEQPDSLQPAAEALGLKTATTDWISRDSAPAPLADERVISALF
                     GDEAVQKSRNTEAIEIGNGTLVSARVKAFEPARRLPLDEVRERIVELVRIEQGRVKAT
                     RAGEEMLAALRRGEQPAARWGEARKLQRGAPGLPAAAMQAVFSASAETLPVHVGVQST
                     EGDYVLYRIEAVERAQIGDADPRVAAVTGQYKQLLAGRDFGAFLSELRQRYEVTINPT
                     ALQVEPR"
     gene            complement(6409..7206)
                     /gene="fabI"
                     /locus_tag="PKV97_06400"
     CDS             complement(6409..7206)
                     /gene="fabI"
                     /locus_tag="PKV97_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HNB05546.1"
                     /translation="MGFLAGKRILITGLLSNRSIAYGIAKAMHREGAELAFTYQNDRF
                     KDRVAKLAADFGSSLIFPLDVQDDAQITGLFEQLGQHWDGLDALVHSIAFAPGEALEG
                     DFLDGLTREAFRISQEVSTYSFPALVKAARPMMQGRNASVLTLSYLGAVRTMPNYNIM
                     GLAKASLEASVRYLAVTLGPEGIRANAISAGPIKTLAASGIGSFGKLLAFNEHNAPLR
                     RNVTIEEVGNAAAFMCSDLASGITGEVMYVDGGFNTTALGNALPLPQ"
     gene            7543..9741
                     /locus_tag="PKV97_06405"
     CDS             7543..9741
                     /locus_tag="PKV97_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB05547.1"
                     /translation="MLIACLALAACGPVWNDPYPVAERGEHILYTAFTERPKHLDPVQ
                     SYSEDEASFLYQIVEPPLQYHYLKRPYVLEPATAEAMPSLRRLDAGGRELPANADPAK
                     VVRTVVEVRIRPGILYQPHPAFALDEDGSPRYLGLGEADLRAVRGLGDFAATGTRELE
                     ARDYVYQIKRLAHPRLHSPIFELMAEYLPGLQELQAGLVAATKEGGASGAAAKDDPPG
                     WLDLDRFALPGVEVVDRHTLRITLQGAYPQFLYWLSMPFFSPVPREVDRFFAQPGMAE
                     RNLTLDWWPVGTGPYMLVENNPNARMVLARNPNYRGDAYPCAGESGDAEAGLLGDCGK
                     PMPFIDKVVFSREREGIPYWNKFLQGYYDASGVSSDNFDQAVSLTSQGEVTLSDDMRD
                     KGIRLLTSVSPSIFYLGFNMLDPLVGGGASKAEKERARKLRQAISIALDMEEFVSIFL
                     NGRGLPGMSPLPPGIFGARAGRAGMNPVVYEWQGSEADGRPVRRGVDEARRLLAEAGW
                     PNGRDAQTGEPLVIHLDTTPGGLGDKARSDWLAKKFRALGVQFVVRPTDFNRFQDKIR
                     QGNAQLFFFGWNADYPDPENLLFLLHGPQGKVKFNGQNAANYENPEYDALFERMKAMP
                     DGPARQQIIDRMVAMLQHDAPWIFAFHPMSYSLQHGWVLNRKTGAMVRNTMKYQRIDL
                     ERRAAARTEWNAPVTWPLLLVAALLAALVAPAAIHWRRRETATAAPGPRG"
BASE COUNT         1523 a         3290 c         3500 g         1448 t
ORIGIN      
        1 gcctggtggc cgacgccatc cggcgctcgc gcgcgggcct gtcggacgag aaccgtccct
       61 acggctcctt cctcttcctc ggccccaccg gggtgggcaa gaccgagctg tgcaagacgc
      121 tggcgaactt cctgttcgat tccgaggaac acctgatccg catcgacatg agcgagttca
      181 tggagaagca ctcggtggcg cgcctgatcg gtgcgccccc gggctacgtc ggctacgagg
      241 aaggcggcta cctgaccgag caggtgcggc gcaagcccta tagcgtgatc ctcttcgacg
      301 aggtcgagaa ggcgcatccg gacgtgttca acgtgctgct gcaggtgctc gacgacggtc
      361 gcatgacgga cggccagggc cgcacggtgg acttcaagaa caccgtgatc gtgatgacct
      421 ccaacctcgg cagccagatg atccaggcca tggcgggaga cgactacggg gtgatcaagc
      481 tcgcggtgat ggcggaagtg aagacctact tccgtccgga gttcatcaac cgcatcgacg
      541 aggtggtggt gttccacgcg ctggatgaaa agcacatcgc cggcatcgcg aagattcagt
      601 tgcagtacct cgagaagcgc ctggcgcggc tggaaatggg catggagatc agcgatgcgg
      661 cgctcgccga gctcgcgact gccggcttcg acccggtgtt tggcgcacgg cccttgaagc
      721 gggcgatcca ggagcgcatc gagaacccgc tcgcgaaggc gatcctcgaa ggtcgcttcg
      781 ccgccaagga ctgcatccgg gtggatgccg agggcgggca actggtgttc cggcgcgccg
      841 actgaggcgc ggcgcgcaaa gccgcgcccc gatcgcgctt cgtctcgtgc ctgccggccc
      901 gtggcgaagc gcgatcggtg tcatgcgatc gccgccgcct gttcctcgaa ccatgtgcgg
      961 aactcctcta cccccagcgg ccgcgagaac aggtaaccct gcatcgcgcg gcaaccccgc
     1021 ttgcagaggt agccgcgctg ctcctcggtt tccacgccct cggcgaccac ctccatgccg
     1081 aggtcgcggg cgagcgacag ggtggccgag gtgatcgcgg cgtcttccgg attgtccggc
     1141 aggccgtcga cgaaggagcg gtcgatcttc aggcggcgca gcggcaggcg cttgaggtag
     1201 gacaggctgg agtagccggt gccgaaatca tccagggcca gcgtcagtcc gagcgccacg
     1261 agctggtcga agctcgccac cagctcgggc gtcgcgtcca tcagcgcgct ctcggtgatc
     1321 tccagctcga tccgcgccgg atcggcgccc gtctcgccca gcacgcgcgc caccgtggcg
     1381 acgaagtcgc gccggcggaa ctgcagcgcc gagatgttta ccgccacgtc gagcggcggc
     1441 aggccggcgt cgcgccagcg cacctgctgc agacaggctt cgcgcagcac ccattccccg
     1501 agcggcacga tcaggccgct ctgttcggcg aaagggacga agcggtcggg cgggagcaga
     1561 ccccgctcgg gatgttgcca ccgcaccaag gcctcgcagc cgtacacccg gccgctcgcc
     1621 gcgtcgatct ggggctggta gtgcaggcgc agatcgccgc tctcgatcgc cttgcgcagc
     1681 tcggcctcgg tctgcagtcg gcgggtcacg cgctgggtca tctcgggcag gaagaagcgg
     1741 taggcgttgc ggccggactc cttggcctcg tacatcgcag tgtcggcgtg cttgagcagc
     1801 gtatcgatct cgccaccgtc ggcggggtag agggcgattc cgatgctcgc gccgacgcgc
     1861 agcggctggc tgtcgatctg cagcgggcgg gcgagcgcat cgagcacgcg ctcggcaacg
     1921 cgcgccggct cttcggcgtg cgcgaggcgc gacaacagga tcacgaactc gtcgccgccg
     1981 atgcgggcga ccaggtcttc cgcgcgtacg cagccgccga ggcggcaggc cacctgggcg
     2041 agcaggcgat cgccggcggc gtggccgagc gagtcattga ccgtcttgaa gtcgtcgagg
     2101 tcgaggaaca gcacggcgca gcggtcgtca tggcgcgcgg cgtgccgcag cacgtcctgg
     2161 gcgtggtcct gccaggcgct gcggttgagc agcccggtga gcgggtcgaa gcgggccagg
     2221 tagcggatgc gggcttcggc ctcgcgcagc gccgtgacgt cctgcgcggt accgcggata
     2281 cgccggaggc ggccgcccac gtgctcgccc tcgatgcgga actgcagcgt gcgcatcggc
     2341 aggctgtcgg tgttgcagtc gagcatgatc cggcccggct ccgtggcggc gcgctcgagc
     2401 gcgtcggtca gggccgcgcg gctggcgggg gcgatcgcgt cgatcaggtc ggcgagcgag
     2461 ttcaggtcgc ctgccgtgct gtcgagcagc aggtggagct cgtccgaggc gtgcagcccg
     2521 tcgcgtgcgc agagggtctc ccagctgccg aggcgggcga tgcgctgggc gtcgatcagt
     2581 gcggcgcggt tttcatgcaa gcgtacgctc gcggcctgga gctcggcggt gcggtgttcg
     2641 accagcgcct ggatgcggcg acggttgccg gtggtgatga gcaggagcgc gccgagcatt
     2701 gcgctggtga ggacgccgac cgcgatcgtg ccccacgcgg cccagctgcg cagctgttcg
     2761 atgtagcggg gcagggcgcg ggtgtgcagc tcccatcgac gaccggcgaa atcgatgctc
     2821 gtggtgcgcg ccaggatctc ttccggccag accgactcct gtccgcgatc gtcgccggca
     2881 agcagcgccg actcgggccc ggcgtcgcgg tcggagagtc gcaccgcgat gccctgcgcc
     2941 gcagcctggc gtgaaacggc tgcgagcagg tcgtccatgc ccagtgccgc ggacacgact
     3001 ccgagcgggg cgcggaccgg tgcaggcggc gaggaggcga aaacggcttg atagagcacc
     3061 acggcatgct gctccccgtg ctcctgcacc aggcgcatgc gcgcgctggc cgctggctgg
     3121 ccggtggcca gcgcgcgttc gatcgcctcg cctgcgaccg ggaaggacag tggatccatg
     3181 ccgagcacgg cgtggttgcc ctcttcgggc tcgacgtgcg tgaccgggaa atactcgcga
     3241 cgctcggtag caggcgaagt ttgtccggcg ggatcgcggt cgaggatgcg gtaatcgacc
     3301 cggtagtcgc tccggatccc ctcttcgaat gccgcgcgct cagcgtgtct tacgtaaggg
     3361 ttccaggtga agctgcgcgt gcccgggtgc agtcgcatcc agggcgcgac gaactcgtgg
     3421 aaatcggcgc gcgagacctg gtcgctcgcg agcagcaggc gctcgatcga acgcaccatg
     3481 tcgacctgtt cttcgaggcg cttctgcagc agggcggaga gatggacgct gtcgcgctcg
     3541 aactgcgccc gcacgcgcga atcctcccag ctcaccacct gccggtaggc gagggcgagc
     3601 agcacgagcg cgatcgacat cggcagcgcg acgccgagtc tgcgctggcg ccagtcctgc
     3661 gccgggcggc caatgaacac cagggtgagc ggggcggcga cgagcatgcc cagggtgtcg
     3721 ccggtccacc aggtccacca gttgaagagc gcgtcggatg ccgggatcac cgccgcccag
     3781 accagggcgg agacgccgat cgtcgagcac accagcccgc tcagcggcat gcagatgccg
     3841 aggaagcgca tcaccgagcc ggccgtgtcg agcgtgtcgc cgggcgcgag cagccggcgc
     3901 gcgagcagga cgccgaccac cgcctgcagg gtggcgccta ccgggacggc gagcggaccg
     3961 agcggattcc agcctgcgct gccgagcagc ggccaggcgg tggcgagctg gaccagggcc
     4021 gaccctgcga acaccgcagg ccacaggcgc agtccgaaca cgaacagcgc ggcgagtgcg
     4081 ataccggccg gtggaaagaa gggggcggtg tagcccggtg ggatggcgag atggagggcg
     4141 ggatagccga ccgcccaata ggccagggcg aggacgactg cggggagagg gcttgcgcgc
     4201 atccgcgcag tatattgagc gccgttaact gtgcgctgtg acggatggca tgcagcggac
     4261 ccgaagccgg accgatgcgc taaactgcgg aacttttcgc gccgggatcc tgccacacgg
     4321 ctgcattttt ctgtcctttc cacggaatac gagatgttcg aggctgttcg caacaataaa
     4381 cgcgtcgccc aggtgatcct ggcgctcctg atcgtcccct tcgccttctt cggcatggac
     4441 gcctacttca gcgactcggg ttcggcgaac gaggccgcgc gcgtcggcgg caccaccata
     4501 ggcgcatggg agttcgacca ggccctgcgc gagcagcagg accgcctgcg gcaggacggc
     4561 gggggccagg tcgaccgtgc gctgctgcag tcggccgaac tgcggcgagc cgtgctcgag
     4621 aacctgatca accagcgcgt gctcgcgctc tacgccgccg agaaccgtct cgtggtgacg
     4681 ccgcagcagc tgcaggagac catcgccggc gtggcctcct tccaggaaaa cggcagcttc
     4741 tcgctgcagc gctacgagac cctgctgcgt gcccagggca tgacccccgc gactttcgag
     4801 gcgcgcctgg cgcaggacgt gcgggtgcag cagatcgtcg cggcggtcgg cgacgccggc
     4861 atcgttccac aggcatcggc acggcgcttc ctcgacgccc agctggagtc gcgcagcgtg
     4921 cgcgagttcc gcctctcggc gaaggccctg gcggccgacg tcaaggtgga cgacgcgcag
     4981 gtcagcgcct actacgaggc caacccgacg cgcttcgagc gcccggcgcg gctgcaggcc
     5041 gaatacctgg tcttcgaccg cgcggcggtc gagcgtggcg tcgaggtcgc ggacgacgcg
     5101 gtgcgcgcct tctacgacgg caatccgcag cgcttcggcg tgcccgagga gcgccaggcg
     5161 cgtcacatcc tgctcgccct cgaccaaggc gccgagcagg ccgaggtcga tcgcgtgatg
     5221 gccgaggcgc gagcgctcgt cgagcaactg cgcgccgatc ccgcgcgctt cgccgccctc
     5281 gcgaaggaga agtcgcagga tccgggctcg gccggcaacg gcggcgatct cggtttcttc
     5341 gcccgcggcg tgatggtcgg cgccttcgag gacgcggtgt tcggtctgca gaaaggcgtg
     5401 atcggcgagc cggtgcgcag cgacttcggc gtgcacatcg tcgaggtcac cgacatcaag
     5461 ccttcctcgg tcaagccctt cgaggccgtg cgcgcggaga tcctcgccga gctgcgcgcc
     5521 caggaggccg gtcgtcgttt cgcggagctg gccgagcagt tctccaacac ggtgtacgag
     5581 cagcccgaca gcctgcagcc cgcggccgag gcgctcggcc tcaagaccgc caccaccgac
     5641 tggatcagcc gcgactccgc cccggcgccg ctcgccgacg agcgcgtgat ctcggccctg
     5701 ttcggcgacg aggcggtgca gaaaagccgc aacaccgagg ccatcgagat cggcaacggc
     5761 accctggtgt cggcgcgggt caaggccttc gagccggcgc gccggctgcc gctcgacgag
     5821 gtccgcgagc gcatcgtcga actcgtccgc atcgagcagg gccgggtgaa ggccacccgt
     5881 gcgggcgagg agatgctggc cgcgctgcgc aggggcgagc agcccgcagc ccgctggggc
     5941 gaggcgcgca agctgcagcg tggtgccccc gggctgccgg cggcggccat gcaggcggtg
     6001 ttctcggcgt cggccgagac gctcccggtg cacgtcggcg tgcagtcgac cgagggcgac
     6061 tacgtgctgt accgcatcga ggcggtcgag cgcgcgcaga tcggcgacgc ggacccgcgc
     6121 gtcgccgcgg ttacgggaca gtacaagcag ctcctggccg ggcgcgactt cggcgccttc
     6181 ctgagcgagc tgcgccagcg ctacgaggtc acgatcaacc cgacggcact gcaggtcgag
     6241 ccgcgctgat tcccagcgga tccccgcatg ctccgccact cgcccgtgcg gcgggggcag
     6301 catggaaaca aaaagcccgg tgtcgaaacc gggctttttt gtgccgtccg ccgaggctcg
     6361 gcaggcggat gcggcggctg ccgcgcagcg ccgtccgccg cgatatcctt actgcggcag
     6421 cggcagcgcg ttgccgagcg cggtggtgtt gaagccgcca tccacgtaca tgacctcgcc
     6481 ggtgatgccg ctggccaggt ccgagcacat gaaggcggcg gcgttaccca cttcctcgat
     6541 ggtcacgttg cggcgcagcg gggcgttgtg ctcgttgaag gcgagcagct tgccgaagct
     6601 gccgatgccc gaggccgcga gcgtcttgat cgggccggcg gagatcgcgt tggcgcggat
     6661 gccttccggg ccgagggtga cggcgaggta gcgcaccgag gcttccaggc tggccttggc
     6721 gagacccatg atgttgtagt tgggcatggt gcgcacggca cccaggtagg acagggtcag
     6781 cacgctggcg ttgcggccct gcatcatcgg gcgcgcggcc ttgaccagcg ccgggaagct
     6841 gtaggtgctg acctcctgcg agatgcggaa ggcctcgcgg gtgaggccgt cgaggaaatc
     6901 gccctcgagt gcctcgccgg gggcgaacgc gatcgaatgc accagcgcgt cgaggccgtc
     6961 ccagtgctgc ccgagctgct cgaacaggcc ggtgatctgc gcgtcgtcct gaacgtcgag
     7021 cgggaagatc aggctgctgc cgaagtcggc cgccagcttg gcgacgcggt ccttgaagcg
     7081 gtcgttctgg taggtgaagg cgagctcggc gccttcacgg tgcatggcct tggcgatgcc
     7141 gtaggcaatc gaacggttgc tgagcagacc ggtgatgagg attcgtttgc cggcgagaaa
     7201 gcccatgaga aatgactccg aatgacaaga cggggattat agcgaaaaac cgtcaaaacc
     7261 cttcgacgga cgggactttc gcccgcgcgg ggggatggaa ggaggcgacg gagccggtag
     7321 ggcaggccat gtttcgtcga ggcgggcggg tcacggctgt gggtccgagg ccgtgtgcag
     7381 atcgcaagcg cgtgcgaaac ctgccagaat ccgcgctttc atcgaagctt ccgcgtgccc
     7441 gtgcgccggt tctcctgccc tctccggacc cccggccgcc ccggcccgac ccgccctgcg
     7501 cacccgcgca gggcgggtgc atcgttacgt tccgcggctg cgctgttgat cgcctgcctg
     7561 gcactcgcgg cctgcggccc ggtgtggaac gacccctatc cggtggccga gcgcggggaa
     7621 cacatcctgt acaccgcctt caccgagcgc cccaagcatc tcgatccggt gcagtcctac
     7681 agcgaggacg aggcgagctt cctgtaccag atcgtcgagc cgccgctgca gtaccactac
     7741 ctgaagcgcc cctatgtgct cgagcccgcc accgccgagg ccatgccgag cctgcgccgg
     7801 ctcgacgctg gcggccgcga gctgcccgcg aacgccgacc cggccaaggt ggtgcgtacc
     7861 gtggtcgagg tgcgtatccg cccgggcatc ctgtaccagc cccacccggc cttcgccctc
     7921 gatgaggatg gcagcccgcg ctatctcggc cttggcgagg ccgacctgcg cgcagtgcgg
     7981 ggtctcggcg acttcgccgc gaccggcacg cgcgagctcg aggcgcgcga ctacgtctat
     8041 cagatcaagc gtctcgccca tccgcgcctg cactcgccga tcttcgagct catggcggag
     8101 tatctgcccg ggctccagga gctgcaggcc gggctggttg cggcaaccaa ggaaggcggg
     8161 gcgagcggtg ccgcggcgaa ggacgacccg cccggctggc tggatctcga ccgcttcgcg
     8221 ctgcccggcg tggaggtcgt ggaccgccac accttgcgca tcaccctgca gggcgcctat
     8281 ccgcagttcc tgtattggct ttcgatgccc ttcttcagcc cggtgccgcg cgaggttgac
     8341 cgcttcttcg cccagcccgg catggccgag cgcaacctga cgctggactg gtggccggtg
     8401 ggcaccgggc cgtacatgct ggtcgagaac aaccccaacg cgcgcatggt gcttgcgcgc
     8461 aatccgaact accgcggcga cgcctatccc tgcgcgggcg agtccggcga tgccgaggcc
     8521 gggttgctcg gcgactgcgg caagcccatg ccattcatcg acaaggtggt gttctcgcgc
     8581 gagcgcgagg gcatcccata ctggaacaag ttcctgcaag gctattacga cgcctcgggc
     8641 gtgtcttcgg acaacttcga ccaggccgtc agtctcacca gccagggcga ggtgacgctc
     8701 tcggacgaca tgcgcgacaa gggcatccgc ctgctcacct cggtgtcgcc gtcgatcttc
     8761 tacctcggct tcaacatgct cgacccgctc gtgggcggcg gcgcctcgaa ggcggagaag
     8821 gagcgcgcgc gcaagctgcg ccaggcgatc tcgatcgcgc tcgacatgga ggagttcgtg
     8881 tcgatcttcc tcaacggccg cggcctgccc ggcatgagcc ccttgccgcc gggcatcttc
     8941 ggcgcgcgcg cgggacgggc ggggatgaac ccggtggtgt acgagtggca gggcagcgag
     9001 gcggacggcc gtcccgtgcg ccgtggcgtc gacgaggcgc gccgcctgct cgccgaggcc
     9061 ggctggccaa atggacgcga tgcgcagacc ggcgagccgc tggtgatcca cctcgacacc
     9121 acgcccggcg ggctgggcga caaggcgcgc tcggactggc tggcgaagaa gttccgcgcg
     9181 ctgggcgtgc agttcgtcgt gcggccgacc gacttcaacc gcttccagga caagatccgc
     9241 cagggtaacg cgcagctctt cttcttcggc tggaacgcag actaccccga tccggagaac
     9301 ctgctcttcc tgctccacgg cccgcagggc aaggtgaagt tcaacggcca gaacgcggcc
     9361 aactacgaga acccggaata cgacgcgctg ttcgagcgca tgaaggccat gcccgacggc
     9421 cccgcgcgcc agcagatcat cgaccgcatg gtggcgatgc tgcagcacga cgcgccgtgg
     9481 atcttcgcct tccacccgat gtcgtattcg ctgcagcatg gctgggtgct caaccgcaag
     9541 accggggcca tggtgcgcaa caccatgaag taccagcgca tcgacctcga gcgccgtgcc
     9601 gctgcgcgca cggagtggaa cgcgccggtg acctggccgc tgctgctggt ggcggcgctg
     9661 ctcgccgcgc tggtggcgcc ggcggcgatc cactggcgcc ggcgcgagac cgcgaccgcg
     9721 gcgccgggac cccgcggatg agggggcgaa gacgccgcgc t
//
LOCUS       DAOQNN010000081         9687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3350_length_9687_cov_9.783119, whole genome shotgun sequence.
ACCESSION   DAOQNN010000081 DAOQNN010000000
VERSION     DAOQNN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3350_length_9687_cov_9.783119"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1170
                     /locus_tag="PKV97_06410"
     CDS             97..1170
                     /locus_tag="PKV97_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNB05548.1"
                     /translation="MTSWTPNRSPVAAPHVHGRSNIPQVMRTVLLALLPATATGVFLF
                     GWPALATVLLAIAGCVAAEAAALAIGKRPLRHTLADGSAVVTGLILALCLPPWAPWWL
                     PLLGGAFAIVVGKQVFGGLGQNVLNPAMLARVALLVSFPLEMTAWVAPQPLFSPTAPG
                     LLDGLAITFGGGAVPDAMSAATALAPLKHGGDFAAGEALLALALGTVPGSLGETSALA
                     LLAGGALLVARRIVPWQIPAALLGTLALLAALFNQIDPAHYPPPLLHLLGGSAVMCAF
                     FIATDPVGAPVTPLGRAIFGAGIGALIYVIRTWGGYPEGVAFAVLLMNAATPLIDRLV
                     RPRRYGRLRTGNPIPLAPKEDGR"
     gene            1167..1823
                     /locus_tag="PKV97_06415"
     CDS             1167..1823
                     /locus_tag="PKV97_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNB05549.1"
                     /translation="MSSIAAGGLRQRPGLLLGAFALGCSAMLGMGDLATHEEIRLRQA
                     EDLRNALDQTLSAVPHDNNPAADTVAVEDEGRAVTVYRARLAGEVRGVAFNWVSEAGY
                     GGPITLTVGIGRDGRIASVRVLSHSETPGLGDKIDVRKSDWIRGFDGRGLDTGTRWAV
                     RKDGGEFDQFAGATITPRAVVGAVHASLKFFAGNRARLLAAPAGPGGDAAATRSETPS
                     "
     gene            1820..2500
                     /locus_tag="PKV97_06420"
     CDS             1820..2500
                     /locus_tag="PKV97_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNB05550.1"
                     /translation="MSEVRRVVRDGLWDNNVVFAQMLGMCPTLATTGSATNGLGMGLA
                     TTVVLVSSGVLVSLFRNVIAREVRIAIYVLLMSTVVTLVDMAMNAWLHELHKVLGLFI
                     PLIVVNCAIMGRAEAFASKRAPGMAALDGLMMGIGFTLALMVLGGVREAIGQGTLFAD
                     ASMLLGQHFAFLELRLEGYRGVLLMILPPGGFLALGFLVAARQRMLARARARSLGASA
                     GAGITAGT"
     gene            2539..2853
                     /locus_tag="PKV97_06425"
     CDS             2539..2853
                     /locus_tag="PKV97_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNB05551.1"
                     /translation="MKISVSYAHPEKPAWMRLDVDEGCTLRAAIERSGILARYEDIDL
                     ERNKVGIFGKIKPLETVLAEGDRVEIYQPIVIDPKTAPRRKRTGADGGDGTEGAERPE
                     GD"
     gene            complement(2883..3341)
                     /locus_tag="PKV97_06430"
     CDS             complement(2883..3341)
                     /locus_tag="PKV97_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNB05552.1"
                     /translation="MNQEIAGRAGRVRESANATTYTISDLAKEFDITTRAIRYYEDQG
                     LISPARRGSRRIYSRRDRVRLSLILRGKRLGFSLQETRELFDLYDNAPGEQAQMSHFM
                     ELLGARRAMLERQMEDIRAILDEIGAAEKEALRSIAARAPHPKAATSPSA"
     gene            complement(3554..4195)
                     /locus_tag="PKV97_06435"
     CDS             complement(3554..4195)
                     /locus_tag="PKV97_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB05553.1"
                     /translation="MARGRAPTFQLQRATILDAAAGLFAAEGFHNASMAEIARACGVS
                     KALLYHYYRDKEHILYDIAAGHVEDLLAIVADVEAAAESPRERLERLILRFMQAYEGG
                     RRQHVVLIQDVKFLSPEQRARIHEQERRVVHAFALAIEAVEPGLRVVALDKAVTMALF
                     GMMNWTFTWLRPDGPLSYEDMARVVARIFLQGVAGFAAQAGHPSAAAVEIALP"
     gene            4651..5259
                     /gene="paaY"
                     /locus_tag="PKV97_06440"
     CDS             4651..5259
                     /gene="paaY"
                     /locus_tag="PKV97_06440"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HNB05554.1"
                     /translation="MPCYEIDGVRPVVHPTAYVHPDAVLIGDVFVGAGCYVAPLASLR
                     GDFGRIVLEEGSNVQDACVLHSFPGIDTVVEVNGHVGHAAVLHACRIGRNALVGMNAV
                     VMDHAEVGEGSIIAAAAFVKAEMKIPPGVLVAGVPAKVLRELTEQERKWKVIGTDCYQ
                     NLARRSLATLKPCDPLREGDRERPRFEAPDVMPLSDARRRAG"
     gene            complement(5287..6225)
                     /locus_tag="PKV97_06445"
     CDS             complement(5287..6225)
                     /locus_tag="PKV97_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB05555.1"
                     /translation="MSLRDFDLNLLRVLLAVYEAGSVSRAAEHLGLSQPTVSSALGRL
                     RRALGEPLFIKTASGVTPTARTHALASSARAMLARLETDFFVADRFDPAKYEGNLTVA
                     LSDVGELVFLPRILDSVRRLAPRAMLDSVTLAPGELMRAMEDGEVDLAIGYYPDLDAA
                     GFYREKLLDHRFVCLLRADHPVQDRQLSLERFLSLEHAAVRAKGRSQEIFEKYLERLR
                     IQRRIVLRTPHFMSLPGIIGRSDLVVTLPHAIGHYFTRVGEKVRMAEPPLAIPHIELY
                     QHWHRGFHHDPRNRWLRTLVAGLFSDGQDEWPYPFA"
     gene            6436..7869
                     /locus_tag="PKV97_06450"
     CDS             6436..7869
                     /locus_tag="PKV97_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB05556.1"
                     /translation="MNAAEWLLAGDPARIALIEGDVRIDYAGLRADSIRSAAALLDAG
                     LRPGDACVLALPDGIEWATAFIGMLWAGVRPIAINPRTATTQLADLMLDSGAAAALLE
                     DEAARALGDKRAIDLGEWRRRVERATATPAAAQAADDDPAFLLYSSGTTGRPKGILHA
                     HRAIRDAHVFARDLLGARPEHRFYSSSKLFFAYPLANAFFAGLRLGATVVLDPEWPDP
                     ARVAAMVERHEPHIFFSVPTLYRRLIDAGVRFRGVHAAVSAGEACPPALARDWQAMTG
                     VPLVNGYGTTETLSLVLYRTPEMDAAYPTPLTEIHPEQLTSGELETWRLWFSHPAVAL
                     GYTRVVTHDSARFADGRFAPGDVFRRAPDGEGWLFAGRSDQLVKVFGRWVDVVAVEQA
                     VQERMRGKAEEVCVIPAQGEDADMIRLHLFAIPGDLPPPQVLAAAQAAIESLPPYQRP
                     EKIHLVDHFPRTDTGKLRRNELARSTG"
     gene            complement(7903..8226)
                     /locus_tag="PKV97_06455"
     CDS             complement(7903..8226)
                     /locus_tag="PKV97_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNB05557.1"
                     /translation="MKVILDLCVVPLGVGLSVSSYVAACERVIREAGLVSQLHAYGTN
                     IEGEWDEVMAAVKRCHEVVHAMGAPRITTSIRLGTRTDREQTMQDKIDSVERALDRAA
                     SGPGA"
     gene            complement(8263..8859)
                     /locus_tag="PKV97_06460"
     CDS             complement(8263..8859)
                     /locus_tag="PKV97_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNB05558.1"
                     /translation="MLLNKPFGVLCQFTDEAGRRTLKDYVEVPGVYAAGRLDADSEGL
                     VLLTGDGALQHRIADPRHKLAKTYLVQVEGEIDEAALAILRAGVDLGDFRSLPCAARR
                     MPAPDWLWPRDPPVRFRKSVPTSWIEIVLCEGKNRQVRRMTAKVGHPTLRLVRVAIGP
                     WTLAGLGVGEWRELAGAEIAEALPAGAPAAARRGRLRR"
     gene            9032..9520
                     /locus_tag="PKV97_06465"
                     /pseudo
     CDS             9032..9520
                     /locus_tag="PKV97_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886403.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9600..9687)
                     /locus_tag="PKV97_06470"
     tRNA            complement(9600..9687)
                     /locus_tag="PKV97_06470"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9651..9653),aa:Ser,seq:tga)
BASE COUNT         1454 a         3504 c         3240 g         1489 t
ORIGIN      
        1 gctggccgag gcgcgccggc tgcggctggc cgccgaagcc gccgccaagg ccgcgcgccg
       61 gcccgcccgt ccggccgaga cggtggagac cgaatcatga cgagctggac cccgaaccgc
      121 tccccggtgg cggcgcccca cgtgcatggg cgcagcaata ttccgcaggt catgcgcacg
      181 gtcctgctcg ccctgctgcc ggcgaccgcg accggcgtgt tcctgttcgg ctggccggcg
      241 ctcgccaccg tgctgctggc gatcgccggc tgcgtggcgg ccgaggcggc ggccctggcg
      301 atcggcaagc gcccgctgcg ccacacgctc gccgacggtt cggccgtggt gaccgggctg
      361 atcctcgcgc tctgcctgcc gccgtgggca ccgtggtggc tgcccctgct gggcggcgcg
      421 ttcgcgatcg tggtcggcaa gcaggtgttc ggcggcctcg gccagaacgt gctcaacccg
      481 gcgatgctgg cgcgggtcgc gctgctggtg tccttcccgc tcgagatgac cgcctgggtg
      541 gcgccgcagc cgctgttctc gcccacggcg cccggcctgc tggacggcct cgcgatcacc
      601 ttcggcggtg gcgcggtgcc ggacgccatg agcgcggcga ccgcgctcgc cccgctcaag
      661 cacggcggcg acttcgccgc cggcgaggcg ctgctcgcgc tggcgctcgg cacggtaccg
      721 ggcagcctgg gcgagacctc cgcgctcgcg ctgctcgctg gcggcgccct gctggtggcg
      781 cgccgcatcg tgccctggca gatccccgcg gccttgctcg gcacgctcgc gctgctcgcg
      841 gcattgttca accagatcga ccccgcgcac tacccgccgc cgctgctcca cctgctcggc
      901 ggcagcgcgg tcatgtgcgc gttcttcatc gccaccgatc cggtgggcgc cccggtcacc
      961 ccgctcgggc gggcgatctt cggcgcgggc atcggcgcgc tgatctacgt gatccgtacc
     1021 tggggcggct atcccgaggg ggtcgccttc gcggtcctgc tgatgaacgc ggcgacgccg
     1081 ctgatcgatc gcctggtgcg tccgcgccgc tacggccgcc tgcgcacggg caacccgatc
     1141 cccctggccc cgaaggagga tggccgatga gctcgatcgc tgccggcggc ctgcgccagc
     1201 gcccggggct gctgctcggg gcattcgccc tcggctgcag tgccatgctg ggcatgggcg
     1261 acctcgccac ccacgaggag atccgcttgc gccaggccga agacctgcgc aacgccctcg
     1321 accagaccct ctccgccgtg ccccacgaca acaatccggc cgcggacacg gtcgcggtcg
     1381 aggacgaggg ccgcgcggtc acggtgtatc gcgcgcgcct ggcgggcgag gtgcgcggtg
     1441 tggccttcaa ctgggtgagc gaagccggct acggcggacc gatcacgctc accgtcggca
     1501 tcggccgcga cgggcgcatc gccagcgtgc gcgtgctctc gcacagcgag acgccgggcc
     1561 tgggcgacaa gatcgacgtg cgcaagtcgg actggatccg cggcttcgac ggccgcggcc
     1621 tggacaccgg cacacgctgg gcggtgcgca aggacggcgg cgaattcgac cagttcgccg
     1681 gcgccaccat caccccgcgc gcggtggtgg gggccgtcca tgccagcctg aaattcttcg
     1741 ccggaaaccg cgcgcgcctg ctcgccgcgc cggccggccc cggtggcgac gccgcggcaa
     1801 ctcgatcgga gaccccgtca tgagcgaagt gcgcagggtc gtccgcgacg gcctgtggga
     1861 caacaacgtg gtgttcgcgc agatgctggg catgtgcccg accctggcca ctaccggcag
     1921 cgccaccaac gggctcggca tgggcctggc caccaccgtg gtgctggtga gctccggtgt
     1981 gctcgtctcg ctgttccgca acgtcatcgc gcgcgaggtg cggatcgcga tctacgtgct
     2041 cctgatgtcc accgtggtca ccctggtcga catggcgatg aacgcctggc tgcacgagct
     2101 gcacaaggtg ctcggcctgt tcatcccgct gatcgtggtc aactgcgcga tcatggggcg
     2161 cgccgaagcc ttcgcctcca agcgcgcgcc cgggatggca gcgctcgacg gcctcatgat
     2221 ggggatcggc ttcaccctcg cgctgatggt gctgggcggg gtgcgcgagg cgatcggcca
     2281 gggcaccttg ttcgccgatg cctcgatgct gctcggccag cacttcgcct ttctcgaact
     2341 gcgcctcgag ggctaccgcg gcgtgctgct gatgatcctg cccccgggtg gcttcctcgc
     2401 cctcggcttt ctggtcgcgg cacgccagcg catgctcgcc cgcgcccgcg cgcgcagcct
     2461 cggcgccagc gcaggtgcgg ggatcaccgc gggcacctga tcgaagcgca atcgcacact
     2521 gcactgcagg agggaaggat gaagatcagc gtcagctacg cgcaccccga gaaaccggcg
     2581 tggatgcgcc tcgatgtgga cgaaggctgc acgctgcgtg cggcgatcga gcgctcgggc
     2641 atcctcgcgc gttacgagga catcgacctg gagcgcaaca aggtggggat cttcggcaag
     2701 atcaagccgc tcgagaccgt gctcgccgaa ggcgaccggg tggagatcta ccagccgatc
     2761 gtgatcgacc ccaagaccgc gccgcgccgc aagcgcacgg gcgcggacgg cggcgacggg
     2821 accgaggggg ccgagcggcc tgaaggggac tgatcgggcg gcccggatgc gggcgcgcgc
     2881 agctaggcgc tcgggctcgt cgccgccttc ggatgcggtg cccgtgcggc gatcgagcgc
     2941 agcgcctcct tctcggccgc gccgatctcg tcgaggatgg cgcggatgtc ttccatctgg
     3001 cgttcgagca tcgccctgcg cgcaccgagc agttccatga agtgcgacat ctgtgcctgc
     3061 tcgccgggtg cgttgtcgta gaggtcgaag agttcgcgcg tctcctgcag cgagaaaccc
     3121 agccgcttgc cgcgcaggat caggctcagg cgcacgcggt cgcggcggct gtagatgcgc
     3181 cggctgccgc gacgggccgg gctgatcagg ccctggtcct cgtaatagcg gatcgcgcgc
     3241 gtggtgatgt cgaactcctt ggcgagatcg gatatcgtat acgtcgttgc attcgccgat
     3301 tcgcgcaccc ggccagccct tccggcgatc tcttgattca tttcattctc cgttctgcga
     3361 tgtccgccca tgccccattc tcacgccgct cgcacgacgg ggctcgcacc gggctcgcat
     3421 gcaatcgatc cgacctggat gattgcacag aagttccgct aacgtaaacg tcaacgaacg
     3481 tccgcgcaag gacttgcgca gccgtgtgat ccgggcgcca ccgggaagtc gttgtccgcg
     3541 gcgacccggg ccttcaaggc agcgcgatct cgaccgccgc ggcggacgga tggcccgcct
     3601 gcgcggcgaa gcccgcgacg ccctgcagga agatccgcgc caccacccgg gccatgtcct
     3661 cgtaggacag cggaccatcc gggcgcagcc aggtgaaggt ccagttcatc atcccgaaca
     3721 gcgccatcgt gaccgccttg tcgagcgcca cgacgcgcag gccgggctcg accgcctcga
     3781 tcgcgagcgc gaaggcgtgc accacccggc gttcctgttc gtggatgcgc gcgcgctgct
     3841 cggggctgag gaacttgacg tcctggatca gcacgacgtg ctgccgccgc ccgccctcat
     3901 atgcctgcat gaagcgcagg atcaggcgct cgagacgctc gcgcggcgac tctgccgcgg
     3961 cctcgacatc cgccacgatc gccagcagat cctcgacgtg gcccgcggcg atgtcgtaga
     4021 ggatgtgttc cttgtcgcgg tagtagtgat agagcagcgc cttggaaacc ccgcacgcgc
     4081 gcgcgatctc cgccatcgag gcgttatgga agccttcggc cgcgaagagg cccgcggcgg
     4141 cgtcgaggat cgtcgcgcgc tgcaactgga aggtcggcgc cctgcctctg gccatcgggg
     4201 tctcgctcct gtgctgcggc cgctccgtgg tgcgcaggcg cacgcacgcc gaccgggttg
     4261 tccggggcac accgaggggg cacgccgcac gccctcggcg cctgtcgtcg ccgatcgaga
     4321 ggatcgtccg catcgcctcg cccctgcatc cttgcgggtc gcgggctggt gcttgcgctc
     4381 gatcgccgtt aacaggattg tgcccggcaa cagatccgac caaccagtcg atcaataaca
     4441 atgttgcgct acatcaagac acgagtggtt ttcttgcacc ccggcttgcg tgccgcgccc
     4501 ttcccatgca ctcacgcgcg ccctgcaccg cagccgggac cggggcccgt cctcgatccg
     4561 caggccgcgc atcgacgcga ggggcgacgc gccccgcgca ccggcatgct actgtcggcc
     4621 gcacgagtcc catcccatcg gagcccagcc atgccctgct acgaaatcga cggcgtccgc
     4681 ccggtcgtcc atcccaccgc ctacgtgcat cccgacgctg tgctgatcgg cgacgtgttc
     4741 gtcggcgccg gctgctacgt cgccccgctc gcctcgctgc gcggcgactt cggccgcatc
     4801 gtgctcgagg agggcagcaa tgtgcaggac gcttgcgtgc tgcacagctt tcccggcata
     4861 gacaccgtgg tcgaggtcaa cggccatgtc ggccatgccg cggtgctgca cgcctgtcgg
     4921 atcgggcgca acgcgctggt cggcatgaac gcggtggtga tggaccatgc ggaggtgggc
     4981 gaaggcagca tcatcgccgc cgccgccttc gtgaaggcgg aaatgaagat cccgcccggc
     5041 gtgctggtgg cgggtgtgcc ggccaaggtg ctgcgcgagc tcaccgagca ggagcgcaag
     5101 tggaaggtga tcggcaccga ctgctaccaa aacctcgccc gccgcagcct cgccaccctg
     5161 aagccctgcg acccgctgcg cgagggcgac cgcgaacgtc cgcgcttcga ggcacccgac
     5221 gtcatgccgc tgagcgacgc gcgccggcgt gccggctgaa cgctcgacgc ggctgcgccg
     5281 gaccgctcac gcgaagggat agggccactc gtcctgtccg tcggagaaca gccccgcgac
     5341 cagcgtgcgc agccagcggt tgcgcgggtc gtggtgaaaa ccgcggtgcc agtgctggta
     5401 caactcgatg tgcgggatcg ccagcggcgg ctcggccatg cgcaccttct cgcccacacg
     5461 cgtgaagtaa tgaccgatcg catgcggcag cgtcaccacc aggtccgagc gcccgatgat
     5521 gccgggcagg ctcatgaagt gcggggtgcg cagcacgatg cggcgctgga tgcgcagacg
     5581 ctcgaggtat ttctcgaaga tctcctggct gcgccccttg gcacggaccg cggcgtgctc
     5641 gagcgagagg aagcgctcga gcgacagctg acggtcctgc accggatgat cggcacgcaa
     5701 caggcacacg aagcggtggt cgagcagctt ctcgcggtag aagccggcgg cgtcgaggtc
     5761 ggggtaatag ccgatcgcca gatcgacctc tccatcctcc atcgcgcgca tcagctctcc
     5821 cggcgcgagc gtcaccgaat cgagcatggc ccgcggcgcg aggcggcgca cgctgtcgag
     5881 gatgcgcggc aggaagacca gctcgcccac gtccgagagg gcgacggtga ggttgccctc
     5941 gtatttcgcc gggtcgaagc ggtcggcgac gaagaaatcg gtttccagcc gcgccagcat
     6001 cgcccgcgcg ctgctcgcca gcgcatgcgt gcgcgcggtg ggcgtgacac ccgacgccgt
     6061 cttgatgaac agcggctcgc cgagcgcgcg gcgcaggcgg ccgagcgcac tgctcacggt
     6121 cggctggctc aggccgaggt gctccgcggc acgactcacg ctgccggcct catagaccgc
     6181 gagcaggaca cgcagcaggt tcaggtcgaa gtcgcgcaaa ctcatgctgg cccgagtgtt
     6241 atcgataggg tcaataaagg taatccattc attggtattg tgcagcatcg cccgggggaa
     6301 tttaatggtc cggacaaaca gatgacgtcc ccgcccgcga ggggagtcct gccgaggaga
     6361 cacaccgatc atgtccaccc atcccatttc gggccacgcg cccgcgctgc gaaccaccgt
     6421 ctgcggagcg ccatcatgaa cgccgccgaa tggctgctcg ccggcgatcc cgcgcggatc
     6481 gcgctgatcg agggcgatgt ccgcatcgac tacgccggcc tgcgtgccga ttccatccgc
     6541 agcgccgcgg ccctgctcga cgccgggctg cgccccggcg acgcctgcgt gctcgcgctg
     6601 cccgacggca tcgaatgggc caccgccttc atcggcatgc tgtgggcggg ggtgcggccg
     6661 atcgcgatca acccgcgcac cgccaccacg caactcgccg acctgatgct ggactcgggc
     6721 gccgccgcgg cgctgctcga ggacgaagcc gcgcgtgcgc tcggcgacaa gcgcgcgatc
     6781 gacctcggcg aatggcgccg ccgggtggag cgcgccaccg cgacgcccgc agccgcgcag
     6841 gcagccgacg acgatccggc gttcctgctg tactcctccg gcaccaccgg ccgccccaag
     6901 ggcatcctcc atgcccaccg cgcgatccgt gatgcccacg tgttcgcccg cgaccttctc
     6961 ggtgcgcgcc cggagcaccg cttctattcc agctccaagc tgttcttcgc ctacccgctc
     7021 gccaacgcct tcttcgccgg cctgcgcctg ggcgccaccg tcgtgctcga ccccgaatgg
     7081 cccgacccgg cgcgcgtcgc ggcgatggtc gagcgccacg agccgcacat cttcttcagc
     7141 gtgcccaccc tctaccgccg cctgatcgat gccggcgtgc gtttccgcgg cgtgcacgcc
     7201 gcggtctccg ccggcgaggc ctgtccgccc gcgcttgcgc gcgactggca ggcgatgacc
     7261 ggagtgccgc tggtcaatgg ctacggcacc accgagacgc tgtcgctggt gctctaccgc
     7321 acgccggaga tggacgccgc gtaccccact ccactcaccg agatccaccc ggagcagctc
     7381 accagcggcg agctcgagac ctggcggctg tggttctccc accccgcagt cgcgctcggt
     7441 tatacgcgcg tggtcaccca cgacagcgcg cgcttcgccg acggccgctt cgcccccggc
     7501 gacgtgttcc gccgtgcccc cgacggggaa ggctggctgt tcgccggccg cagcgaccag
     7561 ctcgtcaagg tgttcggccg ctgggtggac gtggtcgcgg tcgagcaggc cgtgcaggaa
     7621 cgcatgcgcg gcaaggccga ggaggtgtgc gtgatcccgg cgcagggcga ggacgcggac
     7681 atgatccgcc tgcacctctt cgccattccc ggcgacctgc cgccaccgca ggtgctggcc
     7741 gccgcgcagg ccgcgatcga gagcctgccg ccctaccagc gccccgagaa gatccacctc
     7801 gtggaccact tcccgcgcac cgacaccggc aagctgcgcc gcaacgagct cgcgcgcagc
     7861 accggctgaa gcgcgtcctt gccgagcgcg gccccggacc tgtcaggcac cggggccgct
     7921 cgccgcccgg tcgagcgccc gctcgacgct gtcgatcttg tcctgcatcg tctgctcgcg
     7981 gtcggtgcgc gtgcccaggc ggatcgaggt ggtgatgcgc ggcgcgccca tcgcatggac
     8041 gacctcgtgg cagcgcttga ccgcggccat cacctcgtcc cactcgccct cgatattggt
     8101 accgtaggca tggagctgcg acacgaggcc cgcctcgcgg atcacccgct cgcaagccgc
     8161 cacataggat gaaaccgaca gccccacgcc gagcggaacc acgcacagat cgagaatgac
     8221 tttcacggac gcctccacca gcgctcttcg aaacgcccga ttctagcgcc gcaaccggcc
     8281 cctgcgcgcc gccgccggag caccagcggg cagggcctcc gcaatttccg cgcccgccag
     8341 ctcgcgccac tcgcccacgc ccagcccggc caaggtccag ggcccgattg cgacccgaac
     8401 cagccgcaac gtgggatggc cgaccttggc cgtcatccgg cgtacctggc ggttcttgcc
     8461 ctcgcacagc acgatctcga tccagctcgt cggcaccgac ttgcgaaagc gcacgggggg
     8521 atcgcgcggc cacagccagt cgggcgccgg catgcggcgc gcggcgcacg gcaagctgcg
     8581 gaagtccccc agatccaccc ccgcacgcag gatcgccagc gcggcctcgt cgatctcccc
     8641 ctccacctgc accagatagg tcttggccag cttgtggcgc ggatcggcaa tgcggtgctg
     8701 cagggcaccg tcgccggtga gcagcaccag gccctcgctg tcggcatcga ggcggcccgc
     8761 ggcatagaca ccgggcacct ccacgtagtc cttcagcgtg cggcgaccgg cttcgtcggt
     8821 gaactggcac agcacgccga acggcttgtt gagcagcaag aggcgtgtct cagctcgtcc
     8881 ttcggcagga gtgtcggatt tttttacacc cgatgaaggc tcgcacttgg gcactcggtt
     8941 cggcaaatcc atgaaattcc tgtgtttcct cattccaggg gcttcttagg catgtcggca
     9001 agaattccat aacgatgaat ttggccggga gcatgcgcga tgccgtttcg tcgatgtcag
     9061 caaggggctg cccgccggca agcaccgccg cgcccacgaa gtgctggtcc tgatcggcaa
     9121 gctctacgcc atcgagaaga acacgcgcaa actcaaggcc accgagtggc tcgcgctacg
     9181 ccagggccga agccgcgctg tcatcaatga actgcgctgc tgtcgtgacc aagtgctccc
     9241 taccgtgcca ccgtcctcgg tgctcggagg tgcgctgggc gacttgcatc ggcagtggcc
     9301 gcgtccgacg cgctacctcg agcgcggcga tctgccgatc gacaacaacc cggccgaaaa
     9361 cgccaaggcc aacggcgtcg agccgtacct gtggctgcgc caagtgctgc gcgccttgcc
     9421 caccacgacc accgtcgaac acttcgaggc cctcctgcct tggaatctcg aggcggagca
     9481 gttgatcaca tacggagtga gaattctcca caaacaataa agccccggct agcggggctt
     9541 tttgtcgcca tcttcaagct gctgcgcgct caatggaatg cgctgaatgc ctttattatt
     9601 ggcggaagct cagggattcg aaccctggaa cccttgcggg ttgccggttt tcaagaccgg
     9661 tgcaatcgac cactctgcca agcttcc
//
LOCUS       DAOQNN010000082         9648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3377_length_9648_cov_10.597832, whole genome shotgun sequence.
ACCESSION   DAOQNN010000082 DAOQNN010000000
VERSION     DAOQNN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3377_length_9648_cov_10.597832"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="PKV97_06475"
     CDS             <1..129
                     /locus_tag="PKV97_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNB05559.1"
                     /translation="TKGLTLPLMSFGGSGIVANCVALAILLRVDWENRQLLRGGRA"
     gene            126..1196
                     /gene="murG"
                     /locus_tag="PKV97_06480"
     CDS             126..1196
                     /gene="murG"
                     /locus_tag="PKV97_06480"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNB05560.1"
                     /translation="MKTLMVMAGGTGGHIFPGIAVAEELRARGWRIVWMGNPEGMEAR
                     IVPPRGYDTAWVRFGALRGKGLLRKLLLPVNLLSGFWQALREIRRVRPDVVLGMGGYI
                     TFPGGMMAALLGRPLVLHEQNSVAGLANRVLARVADRVLSGFPEVLEQAEWMGNPVRA
                     EIAAVLPPAERMAGRSGPLRVLVVGGSLGAAVLNETVPQALLRLPAEQRPVVVHQAGE
                     RQIEALRAAYARAQVDGELRPFIDDMAAAYADADLVICRAGALTVAELAAAGVASLLV
                     PFPYAVDDHQTGNARFLADRGGAYLLPQSELTPDRLAGILSSLDRGRLLHMAENARAL
                     AKPLAAVAVAHVCAELGGGEQA"
     gene            1193..2608
                     /gene="murC"
                     /locus_tag="PKV97_06485"
     CDS             1193..2608
                     /gene="murC"
                     /locus_tag="PKV97_06485"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNB05561.1"
                     /translation="MKHKVRNIHFVGIGGAGMSGIAEVLANQGYRVSGSDLGDNAATR
                     RLHKMGAHVVRGHDAANVADADVVVVSTAVKADNPEVVAARERHIPVVPRAQMLAELM
                     RFKHGIAIAGTHGKTTTTSLVASILAEGGIDPTFVIGGRLNAAGANARLGTGDYLVAE
                     ADESDASFLLLNPVISVVTNIDADHMDTYGHDFGRVKQAFVDFLHRLPFYGVAVLCED
                     DPNVREIIPQVAKQIVRYGFSETANIRAENVRAEGGRMRFDCVRVNGTTSRLSIELNL
                     PGLHNVLNALAAIAVATEVQVPDAAIVKALAEFNGVGRRFQRYGEVALHDQDGDEAGR
                     FTLVDDYGHHPVEMAATIAAARGAFPGRRLVLAFQPHRYTRTRDCFEDFVKVLSTVDA
                     LLLAEVYAAGETPIVAADGRSLARAIRVAGTVEPVFVEEIADMPRMILSAARDGDVVI
                     TMGAGSIGAVPGKLASIEEAA"
     gene            2605..3519
                     /locus_tag="PKV97_06490"
     CDS             2605..3519
                     /locus_tag="PKV97_06490"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNB05562.1"
                     /translation="MKARFGKVAVLFGGSSAEREVSLMSGAAVLAALQAAGVDAHAFD
                     PAERDLHVLKEEGFERAFIALHGRGGEDGTVQGALELMGIPYTGSGVMASALSMDKWR
                     TKMVWLSAGLPTPRYAILEHDSDWDAVVAELGLPIFVKPVHEGSSMGATKVVEAGQLR
                     AAWELAARYDRLVIAEEFICGAELTAPFLEDRALPLVRIVAPGGNYDYQHKYFTDDTR
                     YDCPSGLPAAQEAELQAMVMKCARVLGCRGWGRADLILTEDGRPYLLEMNTSPGMTGH
                     SLVPMSARVAGLDFTALCLEILQGARLG"
     gene            3512..4375
                     /locus_tag="PKV97_06495"
     CDS             3512..4375
                     /locus_tag="PKV97_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HNB05563.1"
                     /translation="MAEAGIRPASIRSARRPAAGGAARAGAERGLWHRPALLNLISDL
                     LTLGAAVALGWALVIWFVSRPLFPLRELVVLTDPGQVTVEQLDYAARLAVQGNFFTVD
                     LDGVKETFEKLPWVRKAEVRRRWPDALELRLEEHEAVAYWTVSESGEARLVNRQGEVF
                     VAASNADMPQFDGPQGSAGWLLARHAEFSSMLQPLGVRLVGLALSAREAWQLQLDNGM
                     TIVLGREQDKSPLMERLRRFIAVWPRVHEQIDIDIKTADLRYAGGFALTPADASVLHQ
                     PVAPAARKGRK"
     gene            4372..5601
                     /gene="ftsA"
                     /locus_tag="PKV97_06500"
     CDS             4372..5601
                     /gene="ftsA"
                     /locus_tag="PKV97_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNB05564.1"
                     /translation="MSKQYNELIVGLDIGTAKVTCMVAEVKPDGRLNVVGLGTQSTNG
                     LKRGVVVNIEATVDAISRVVQEVELMADCKIHEVYTGLAGSHIKSFNSSGTVAIKEKE
                     VSPLDVERVIEVARAMPIPAEQQILHILTQEFIIDGQGGVREPIGMSGVRLEVKVHIV
                     TGAVSAAQNVIKCVRRCGLEVMDLSLQPLASSHAVLTEDEKELGVCMVDIGGGTTDIA
                     VFTQGAIRHTAVIPVAGDQITNDIAMALRTPTAEAEEIKIRHGVAMHHMADPEEMIEV
                     PGVGDRPPRKLSRQALADVIEPRVSELFELVQAELRRSGYEELLSSGIVLTGGSAVMR
                     GMAELAEDVFHMPARVGAPQYDGGLADVVCQPRYATAMGLVMEGAVQRRRGLQARDTR
                     SVKQFFGRMKAWFERNF"
     gene            5743..6885
                     /gene="ftsZ"
                     /locus_tag="PKV97_06505"
     CDS             5743..6885
                     /gene="ftsZ"
                     /locus_tag="PKV97_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNB05565.1"
                     /translation="MFEIVEKEATGTVIKVVGVGGAGGNAVDHMIREGVKGVHFISAN
                     TDAQALKRCLAPVKVQLGITGLGAGSKPEAGRAAAQESRDAISAALEGAHMVFITGGM
                     GGGTGTGAAPVVAEIAKEMGLLTVAVVTKPFDFENRIRVAESGIEELTRHVDSLIVVL
                     NDKLLEVFGDDAGFEECFRSADNVLRSAVGGIAEIINVPGLVNVDFQDVRTAMAEMGR
                     AMMGSAEAAGMDRARIAAEQAAVSPLLEGTELSGARCVLINITASKSLKMSEVRDAVK
                     TVQAFAAPEAFVKYGTVFDDTMEDRIRITVVATGLGAPRAARQPVMQVVQGTGTYGPA
                     MGGAVDVTNLDVPAVMRSGRRTTVEAMSTSGVGTYDIPAFLRRQAD"
     gene            7094..8017
                     /gene="lpxC"
                     /locus_tag="PKV97_06510"
     CDS             7094..8017
                     /gene="lpxC"
                     /locus_tag="PKV97_06510"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNB05566.1"
                     /translation="MIRQRTLKSVVTATGVGLHGGRKVTLTLRPAAPDTGIVFHRVDL
                     DPPLTLPADPYAVCDTRMCSGLEQGGHKVGTVEHLMSALAGLGIDNLHVDVDAPEIPI
                     LDGSSAPFVFLLQSAGIEEQRAAKKFLRVKKAVEYVEGDKWVRLEPYDGFRLAFSIVF
                     NHPAIDSTSTRVEVDFARDSYIRDVARARTFGFMQDVEFMQANGLALGGSLENAIVMD
                     EYRVLNAEGLRYADEFVKHKVLDAIGDLYLCGHPLLASYSAHKAGHALNNQILRVLLE
                     DKEAWELVSFDQEAATPPAVSHQFAPLVALA"
     gene            8142..8330
                     /locus_tag="PKV97_06515"
     CDS             8142..8330
                     /locus_tag="PKV97_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05567.1"
                     /translation="MLIMRLLLLLAVLSIGGSAILWLLTGNPRYKAWAWRALRATLVM
                     LFVLFALFAIERVLAPVA"
     gene            complement(8567..8989)
                     /locus_tag="PKV97_06520"
     CDS             complement(8567..8989)
                     /locus_tag="PKV97_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HNB05568.1"
                     /translation="MTRLLSRFLGGDALARLQDHAARLNRLQAVLDGMLPPQLQGQCR
                     VANLKEGSLVVAARGGAAAVRVRQILPSLLERLQHGGHPVQSIKVKVGTPEQTAPERH
                     APQRQISEAAKAELHDFADSLPVDSPLRASIERLIRRA"
     gene            9074..>9648
                     /gene="secA"
                     /locus_tag="PKV97_06525"
     CDS             9074..>9648
                     /gene="secA"
                     /locus_tag="PKV97_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585942.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNB05569.1"
                     /translation="MISGLLKKIFGSRNDRLVRQYMHTVRKINAFEAEISALSDEALR
                     GKTAEFRQRVADGESLDALLPEAFAVVREAGKRVHGMRHFDVQLVGGMVLHHGKIAEM
                     RTGEGKTLVATLPAYLNALSGKGVHVITVNDYLASRDAEWMGRIYGFLGLTTGCNLSR
                     MSHDQKQAAYAADITYGTNNEFGFDYLRDNMV"
BASE COUNT         1444 a         3343 c         3392 g         1469 t
ORIGIN      
        1 accaagggcc tgaccctgcc gctgatgagc ttcggcggct cgggcatcgt cgccaactgc
       61 gtcgcgctcg ccatcctgct ccgggtggac tgggagaacc gccagctcct gcgtgggggg
      121 cgtgcatgaa gacgctgatg gtgatggccg gcggcaccgg cggccacatc ttcccgggca
      181 tcgcggtggc cgaggagctg cgtgcccggg gttggcgcat cgtctggatg ggcaaccccg
      241 agggcatgga ggcgcgcatc gtgccgccgc gcggctacga caccgcctgg gtgcgcttcg
      301 gtgcgctgcg cggcaagggc ctgctgcgca agctgctgct gccggtgaac ctgctctccg
      361 gcttctggca ggcactgcgc gagatccgcc gcgtccgccc cgacgtggtg ctgggcatgg
      421 ggggctacat cacctttccg ggcggcatga tggccgcgct gctcgggcgt ccgctcgtgc
      481 tgcacgagca gaactcggtc gccggcctgg ccaaccgtgt gctcgcgcgc gtggccgacc
      541 gcgtgctgag cggcttcccc gaggtgctgg aacaggccga gtggatgggc aacccggtgc
      601 gcgccgagat cgccgcggta ctgccgccgg ccgaacgcat ggccggacgc tcgggcccgc
      661 tgcgcgtgct ggtggtgggt ggcagcctgg gcgctgcggt gctcaacgag accgtgccgc
      721 aggcgctgct gcgccttccc gccgagcagc gcccggtggt ggtgcatcag gctggcgaac
      781 gccagatcga ggccttgcgc gcggcgtacg cgcgcgccca ggtcgacggc gagctgcgcc
      841 ccttcatcga cgacatggcg gcggcctacg ccgacgccga cctggtgatc tgccgcgccg
      901 gcgcgctcac cgtggccgag ctcgccgcgg ccggggtggc cagcctcttg gtgcccttcc
      961 cctacgcggt ggacgaccac cagaccggca acgcgcgctt cctcgccgac cgcggcggcg
     1021 cctatctgct gccgcaatcc gaactaacgc ccgaccgcct ggcgggcatc ctgtccagtc
     1081 ttgaccgcgg ccgcctgctg cacatggccg agaacgcgcg cgcgctcgcg aagccgctcg
     1141 cggccgtcgc ggtggcgcac gtgtgcgccg agctgggcgg tggagaacaa gcatgaagca
     1201 caaggtcagg aacatccact ttgtcggcat cggcggcgcg ggcatgagcg gcatcgccga
     1261 ggtgctcgcc aaccagggtt atcgcgtcag cggttcggac cttggcgaca acgccgccac
     1321 ccgccgcctg cacaagatgg gcgcgcacgt ggtgcgcgga catgacgcgg ccaacgtcgc
     1381 cgacgccgac gtggtggtgg tgtcgaccgc ggtgaaggcc gacaacccgg aggtggtcgc
     1441 ggcgcgcgag cgccacattc ctgtggtgcc gcgcgcgcag atgctcgccg agctgatgcg
     1501 cttcaagcac ggtatcgcga tcgccggcac ccacggcaag accaccacca cctcgctggt
     1561 ggcgagcatc ctcgccgagg gcggcatcga ccccaccttc gtgatcggcg ggcgcctcaa
     1621 cgccgccggc gccaacgcgc gcctcggcac gggcgactac ctggtggccg aggcggacga
     1681 gtccgacgcc tccttcctgc tgctcaaccc ggtgatctcg gtggtcacga acatcgacgc
     1741 cgaccacatg gacacctacg gccacgactt cggccgggtc aagcaggcct tcgtcgactt
     1801 cctccaccgc ctgcccttct acggcgtggc ggtgctgtgc gaggacgacc ccaacgtgcg
     1861 cgagatcatc ccgcaggtgg ccaagcagat cgtgcgctac ggcttctcgg agaccgccaa
     1921 catccgcgcc gagaacgtgc gcgccgaggg cgggcgcatg cggttcgact gcgtgcgggt
     1981 caacggcacg acctcgcgcc tgtcgatcga gctcaacctg cccggcctgc acaacgtgct
     2041 caacgcgctc gccgcgatcg cggtggccac cgaggtgcag gtgccggacg cggcgatcgt
     2101 caaggcgctc gccgagttca acggcgtcgg ccggcgcttc cagcgctacg gcgaggtcgc
     2161 gctgcacgac caggacggcg atgaggccgg ccgcttcacg ctggtcgacg actacggcca
     2221 ccacccggtg gagatggcgg cgaccatcgc cgcggcacgc ggcgccttcc ccggccgccg
     2281 cctggtgctg gccttccagc cccatcgcta cacgcgcaca cgcgattgct tcgaggactt
     2341 cgtcaaggtg ctgtcgaccg tggatgcgct gctgctggcc gaggtctatg ccgccggcga
     2401 gaccccgatc gtcgccgccg acggccgctc gcttgcgcgc gcgatccgcg tcgccggcac
     2461 ggtcgagccc gtcttcgtcg aggagatcgc cgacatgccg cgcatgatcc tgtccgccgc
     2521 gcgcgacggc gacgtcgtca tcaccatggg agcgggcagc atcggcgcgg tgccgggcaa
     2581 gctcgccagc atcgaggaag cagcgtgaag gcccggttcg gaaaggttgc ggtgctgttc
     2641 ggcggttcgt ctgccgagcg cgaggtttcg ctgatgtcgg gcgctgcggt gctcgccgcg
     2701 ctgcaggccg cgggggtgga tgcgcacgcc ttcgatcccg cggagcgcga cctgcacgtc
     2761 ctcaaggaag agggcttcga gcgcgccttc atcgccctcc atgggcgcgg tggcgaggac
     2821 ggcacggtgc agggcgcgct cgagctgatg ggcatcccct ataccggcag cggcgtgatg
     2881 gcctccgcgc tgtcgatgga caagtggcgc accaagatgg tgtggctgtc cgccggcctg
     2941 ccgacgccgc gctacgcgat cctggagcac gactccgact gggacgcggt ggtggccgaa
     3001 ctcggcctgc cgatcttcgt caagccggtg cacgagggct cgagcatggg cgcgaccaag
     3061 gtggtcgagg cgggccagct gcgcgccgcg tgggagctcg cggcgcgcta cgaccgcctg
     3121 gtgatcgccg aggagttcat ctgcggcgcc gagctcaccg cccctttcct cgaggaccgc
     3181 gcgctgccgc tggtgcgcat cgtcgccccc ggcggcaact acgactacca gcacaagtac
     3241 ttcaccgacg acacgcgcta cgactgcccc agcggcctgc ccgccgcgca ggaggcggag
     3301 ctgcaggcga tggtgatgaa gtgtgcgcgc gtgctcggct gccgcggctg gggccgcgcc
     3361 gacctcatcc tcaccgagga cggccgcccc tacctgctcg agatgaacac ttctcccggc
     3421 atgaccggcc actcgctggt gccgatgtcg gcgcgcgtgg cggggctgga cttcaccgcg
     3481 ctgtgcctcg agatcctgca gggagcccgc cttggctgaa gccggcatcc gtcccgcgtc
     3541 gatccgctcc gcccgccgtc cggcggcggg cggagcggcg cgcgcgggtg cggagcgcgg
     3601 cctgtggcat cggcccgcgt tgctcaacct gatctccgac ctgctcaccc tgggtgcggc
     3661 ggtggcgctc ggctgggcgc tggtgatctg gttcgtgtcg cggccgctgt tcccgctgcg
     3721 cgagctggtg gtgctcaccg accccggcca ggtcaccgtc gaacagctcg actacgccgc
     3781 ccgcctcgcg gtgcagggca acttcttcac cgtcgacctc gacggcgtga aggaaacctt
     3841 cgagaagctg ccctgggtgc gcaaggccga ggtgcgccgg cgctggcccg acgccctcga
     3901 gctgcgcctg gaagaacacg aggcggtggc ctactggacg gtgtccgaga gcggcgaggc
     3961 gcgcctggtc aaccgccagg gcgaggtttt cgtcgccgct tccaacgccg acatgcccca
     4021 gttcgacggc ccgcaggggt cggcgggctg gctgctcgca cgccatgccg agttctccag
     4081 catgctgcag ccgctcggcg tgcgcctggt cgggctggcc ttgtcggcgc gcgaggcctg
     4141 gcagctgcag ctcgacaacg gcatgaccat cgtgctcggg cgcgagcagg acaagtctcc
     4201 gctgatggaa cggctgcgcc gcttcatcgc ggtgtggccg cgcgttcacg aacagatcga
     4261 catcgacatc aagaccgcgg acctgcgcta tgcgggcggg ttcgcactga ccccggccga
     4321 cgcctcggtg ctccaccagc ccgtcgcgcc cgccgcccgg aaaggcagga aatgagcaag
     4381 caatacaacg aactgatcgt cggcctggac atcggcaccg ccaaggtcac ctgcatggtc
     4441 gccgaggtga agcccgacgg gcgactcaac gtcgtcggcc tcggcaccca gtccaccaac
     4501 ggcctcaagc gcggcgtggt ggtgaacatc gaggccacgg tggacgcgat ctcgcgcgtg
     4561 gtgcaggagg tcgagctgat ggccgactgc aagatccacg aggtctacac cgggctggcc
     4621 ggcagccaca tcaagagctt caactccagc ggcacggtcg cgatcaagga gaaggaggtc
     4681 tcgccgctcg acgtcgagcg cgtgatcgag gtcgcgcgcg cgatgccgat cccggccgag
     4741 cagcagatcc tgcatatcct gacccaggag ttcatcatcg acggccaggg cggcgtgcgc
     4801 gagccgatcg gcatgagcgg ggtgcggctc gaggtcaagg tgcacatcgt caccggcgcg
     4861 gtgtcggcgg cgcagaacgt gatcaagtgc gtgcgccgct gcgggctgga ggtcatggat
     4921 ctgagcctgc agccgctcgc ctccagccac gccgtgctga ccgaggacga gaaggaactc
     4981 ggcgtgtgca tggtcgatat cggcggcgga acgaccgaca tcgccgtgtt cacccagggc
     5041 gcgatccgcc ataccgcggt gatcccggtc gccggcgacc agatcaccaa cgacatcgcc
     5101 atggcgctgc gcacgcccac cgccgaggcg gaggagatca agatccgcca tggcgtggcg
     5161 atgcaccaca tggccgaccc cgaggagatg atcgaggtcc cgggcgtggg cgatcgcccg
     5221 ccgcgcaagc tctcgcgcca ggcactcgcc gacgtgatcg agccgcgcgt ctccgagctc
     5281 ttcgagctgg tgcaggccga actgcgccgc agcggctacg aggagctgct gtcctcgggc
     5341 atcgtgctga ccgggggctc ggcggtgatg cgcggcatgg ccgagctcgc cgaggacgtc
     5401 ttccacatgc ccgcgcgcgt gggcgcgccg cagtacgacg gcggccttgc cgacgtggtc
     5461 tgccagccgc gctacgccac cgcgatgggc ctggtcatgg agggcgccgt gcagcgtcgc
     5521 agggggctgc aggcgcgcga cacgcgcagc gtgaaacagt ttttcgggcg catgaaggcc
     5581 tggttcgaac gcaatttctg aacgggtttt cgggcggttt tcattttgga tgtgggccga
     5641 tcaccatcgc ttatttagta gtaaactccc ggccgaatta ctagatgcag gccggctggc
     5701 gatcggtgcc gcgcggggca gtcaattcag gaggcgaggc taatgtttga aatcgttgag
     5761 aaggaagcca ccggtacggt gatcaaggtg gtcggcgtcg gcggtgcggg cggcaacgcg
     5821 gtcgaccaca tgatccgcga gggcgtgaag ggagtgcatt tcatctccgc caacaccgac
     5881 gcgcaggcgc tcaagcgctg cctcgccccg gtcaaggtcc agctcggcat caccggcctg
     5941 ggtgccggct ccaagccgga agcgggtcgc gccgccgcgc aggagtcgcg cgacgccatc
     6001 tccgccgcgc tcgagggtgc gcacatggtg ttcatcaccg gcggcatggg tggcggtacc
     6061 ggcaccggtg cggcaccggt ggtggccgag atcgccaagg agatgggtct cctgaccgtc
     6121 gccgtggtga ccaagccgtt cgatttcgag aaccgcatcc gcgtcgccga gagcggcatc
     6181 gaggaactca cccgccacgt cgactcgctg atcgtcgtgc tcaacgacaa gctgctcgag
     6241 gtcttcggcg acgacgccgg cttcgaggag tgcttccgct cggccgacaa cgtgctgcgc
     6301 tcggccgtgg gcggcatcgc cgagatcatc aacgtgcccg gcctggtcaa cgtcgacttc
     6361 caggacgtgc gcaccgcgat ggccgagatg ggccgcgcga tgatgggctc ggccgaggcc
     6421 gccggcatgg accgcgcacg catcgccgcc gagcaggccg cggtgtcgcc gctgctcgag
     6481 ggcaccgaac tctccggcgc gcgctgcgtg ctgatcaaca tcaccgcgag caagtcgctg
     6541 aagatgtccg aagtgcgcga cgcggtgaag accgtgcagg ccttcgccgc gcccgaagcc
     6601 ttcgtcaagt acggcaccgt gttcgacgac accatggaag accgcatccg catcaccgtg
     6661 gtggccaccg gactcggcgc gccgcgcgcc gcgcgccagc cggtgatgca ggtggtgcag
     6721 ggcaccggca cctacggccc ggccatgggt ggcgcggtcg acgtgaccaa tctcgacgtg
     6781 ccggcggtga tgcgcagcgg tcgccgcacc accgtcgagg cgatgagcac cagcggtgtg
     6841 ggcacctacg acattccggc cttcctgcgc cgccaggcgg actgacctcc gcccgggtgg
     6901 ccgccgccgc ggcggccacg aaccggaacc cccgcacggg ccgtcgcctc cggcccgtgc
     6961 gggggttccg ccgtttaccg gctggcgtcg ggcgcgcaac atccgcttgc gcaatagccc
     7021 ggatgcatcg gcgcgatcgc cggggggctg cgggggtgca tgttaaaatc gcgcatcgaa
     7081 gaggaaaaag accatgatcc ggcagcgcac cctgaaatcc gtcgtcacgg cgaccggcgt
     7141 cggcttgcac ggcggtcgca aggtgaccct gaccctgcgg ccggcggcgc ccgacaccgg
     7201 catcgtcttc caccgcgtcg acctcgaccc gcccctgacc cttcctgccg atccctatgc
     7261 agtgtgcgac acgcgcatgt gctccgggct ggagcagggc gggcacaagg tcggaacggt
     7321 cgagcacctg atgtcggcgc tggccgggct gggcatcgac aacctgcatg tggacgtcga
     7381 tgcgcccgag atcccgatcc tcgatggcag ctccgccccc ttcgtgttcc tgctccagtc
     7441 ggccggcatc gaggagcaga gggcggcgaa gaagttcctg cgcgtgaaga aggcggtcga
     7501 gtacgtggag ggcgacaagt gggtccggct cgaaccctac gatggcttcc gcctggcgtt
     7561 ctcgatcgtg ttcaaccatc ccgcgatcga cagcacctcg acccgggtcg aggtcgattt
     7621 cgcccgtgac tcctacatac gcgacgtcgc gcgcgcgcgc accttcggct tcatgcagga
     7681 cgtcgagttc atgcaggcca acgggctggc gctcggcggc agcctggaga atgcgatcgt
     7741 gatggacgag taccgcgtgc tcaatgccga gggcctgcgc tacgccgacg agttcgtcaa
     7801 gcacaaggtg ctggacgcga tcggcgacct ctacctgtgc ggccacccct tgctcgcgtc
     7861 gtattcggcg cacaaggccg gccatgcgct caacaaccag atcctgcgcg tgctgctcga
     7921 ggacaaggag gcctgggagc tggtgagctt cgaccaggag gccgcgaccc cgcccgcggt
     7981 ctcgcaccag ttcgcgccgc tcgtagccct cgcatgaacg ggcgcttcgt gtccggtcgt
     8041 atcccggtga tcgcacgcgc cgcgcctggc ctcgatcccg gctgcatgcg cgccgccgct
     8101 gcgggcgagg cggtcgacgc gcgccgtcga ggggcgtacg catgctgatc atgcgcctgc
     8161 tgctgttgct cgcggtgctg tcgatcggtg gctcggcgat cctgtggctg ctcaccggca
     8221 acccgcgcta caaggcgtgg gcatggaggg ccttgcgcgc caccttggtg atgttgttcg
     8281 tgctgttcgc gctgttcgcc atcgagcgcg tgcttgcccc ggtcgcctga cttccgcggg
     8341 gcggtgcgga ccggagcgcc gatatcgcga ctcgcgtcgc tcctacgggg cggcgcggac
     8401 cgcagcgccg atatcgcgac tcgcgtcgct cctacggggc ggtgcggacc gcagcgccgc
     8461 aatcgcgact tgcggcgctc ctacgcgggg tctgcgaggg cgtcgcggtt ctcgtaggag
     8521 cgacgcaagt cgcgatccct ccgttcgacg accggcgtgc gcctcttcag gcgcgccgga
     8581 tcaggcgttc gatggaggcg cgcagcggcg agtcgacggg caggctgtcg gcgaagtcgt
     8641 gcagctcggc cttcgcagcc tccgagatct gtcgctgcgg ggcgtggcgt tcgggagccg
     8701 tctgctcggg cgtgccgacc ttgaccttga tcgactgcac cggatgtccg ccatgctgca
     8761 ggcgctccag caggctgggc aggatctggc gcactcgcac cgccgccgcg ccgccgcgtg
     8821 cggccacgac caggctgcct tccttgaggt tcgcgacccg gcactggccc tgcagctgtg
     8881 gcggaagcat gccgtcgagc accgcctgca gccggttcag gcgcgcggca tggtcttgca
     8941 ggcgggcgag ggcgtcgccg ccgagaaagc gtgagagcag ccgggtcatg gatgcgtgcg
     9001 agaaccgtca cggggagcct gggtgcgcca tgataaactc ccgcctttgc cgaatcgacg
     9061 tcgacccaaa aacatgatct ccggcctgct caagaaaatc ttcggtagtc gcaacgaccg
     9121 cctcgtccgc cagtacatgc acaccgtgcg caagatcaac gccttcgagg cggagatctc
     9181 ggcactgtcc gacgaggcgc tgcgcggcaa gacggccgag ttccggcagc gcgtggccga
     9241 cggagagtcg ctcgacgcac tgctgcccga agccttcgcc gtggtgcgcg aggccggcaa
     9301 gcgggtgcat ggcatgcgcc acttcgacgt tcagctcgtc ggcggcatgg tgctgcacca
     9361 cggcaagatc gccgagatgc gcaccggcga gggcaagacc ctggtcgcca cgctgcccgc
     9421 ctacctcaac gcgctgtcgg gcaagggcgt ccatgtgatc acggtgaacg actacctcgc
     9481 cagccgcgac gccgagtgga tggggcgcat ctacggcttc ctcgggctca ccaccggctg
     9541 caacctgtcg cgcatgtcgc acgaccagaa gcaggccgcc tacgccgccg acatcaccta
     9601 cggcaccaac aacgagttcg gcttcgacta cctgcgcgac aacatggt
//
LOCUS       DAOQNN010000083         9535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3445_length_9535_cov_8.108122, whole genome shotgun sequence.
ACCESSION   DAOQNN010000083 DAOQNN010000000
VERSION     DAOQNN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9535
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3445_length_9535_cov_8.108122"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="torT"
                     /locus_tag="PKV97_06530"
     CDS             complement(<1..1028)
                     /gene="torT"
                     /locus_tag="PKV97_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HNB05570.1"
                     /translation="MLGLALGALPSCAAALAGAQPAAEWPLQRWAGDHRAANGAAETG
                     PDIVPPSPTRGWRLCAIYPHLKDSYWLAVNFGMTEEARALGLGLGVREAGGYGQLARQ
                     RERVAECLEDAAVDALLIGTVSRSGLNDLLAPQLDHRLVLGVVNDIDPQVVRARIAVP
                     WYQLGWTIGRWLAARHPAGGPPARIAWIPGPADADWVGFIDRGFRTGIAGSAVNVVAE
                     RHGDTGRAIQRRLVEAVLDEQPQLDYLVGNAPMAEAAIAELRRRGREGATAIVSSYLT
                     PAVHGGILRGRILAAVTDFPVLQGRLAVRQALRALEGRPIEPYLGPAVELVDKSSLGR
                     FPVQWMLPP"
     gene            1116..4139
                     /locus_tag="PKV97_06535"
     CDS             1116..4139
                     /locus_tag="PKV97_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB05571.1"
                     /translation="MTAPSYVKPRHERRFGLRERLLLGLLVAALGTVLVAVVGWVSFQ
                     RVVSSQQAIVRDTLPAADALHEAVRANARLAALAPRLLRADSAAELDQLRAALGVDVP
                     LLRDRLAALRSPHVEAELRERLLATGDRLAAGLEGMSAAIDQRLRLRAARLERIDTLR
                     AAIDGLDELARIHADNATAQLVSTLTTLLQPDPGQGAPSLAEREAARERVLDLDIDSL
                     ERMHELNLTVHALGFLIGRLDELDSGARLQAARVEFAAHLALLARRLADIADPGGREQ
                     GERVLRLLASALDEEGTFALRAREIALRAQVETLQGVVGELTTELDALAGELIHRGGR
                     ILAAAGSAAERSATSGLIAFGVIAAALLLVTLGVTVHALRRHTLGRLRALEEATLALA
                     SGRREVAIDTAGDDELASLAVALERFRANAIERDRFAEELRQQQQELENQVLARTAQL
                     REANAALARETADHARARHAAEQADRAKTAFLGTVSHELRTPLAGILGLLELVEDASS
                     TTERKQYQAQMRAAAVLLLELLEDMLDFARIEAGGVQVDKASFSLRDTVNDVFAVQGT
                     RAAVRGLALIAEVDPALPDAVQGDRRKLSQILLNLVGNAIKFSDAGAVTVRVRAGTQP
                     DQLHFAVEDHGIGIEPARQQEVFEPFVQVRDSGRHHAGTGLGLAVCRRLVEAMGGAIE
                     LKSAPGQGTTVSFEIPLPATAAAAVEALPAATEPERAALPPGHRVLVVEDDEVNRMVV
                     ERFLDALGQQAVCAPDIQNALRLLQARPIDLALIDMNLPDGDGRELLARLRALPAHAH
                     TPAVLMSAHIPRREVDALLEAGFAAFLSKPFARERLRALLADLLAEPAAAAPTQGTAG
                     VAEVLQATDWIDADFLRAEREALGEETVADIVGVFRTQGQPLIDALLAAARAGEHEAC
                     ARLAHKLRGAAGNVGAGRLAECAGALEEALKPDPKGDAPRAGAIGDLAARAGELGEAW
                     SYTLQALDALRSAANDTEAQRPDQTPPGSTSAASR"
     gene            complement(4100..4828)
                     /locus_tag="PKV97_06540"
     CDS             complement(4100..4828)
                     /locus_tag="PKV97_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB05572.1"
                     /translation="MQAPLESHVTLLVVDDDLVAQARMNAYFSKEGYRVLLAGDGESF
                     WRQLAQGEVDLVLLDINLPGQDGLSLARELRARDASIGIILVTSRNDDVDKIVGLEVG
                     ADDYVTKPFNPRELLARVKSLLRRSATRPAETADSLRFAGWVLELSRRRLRDRAGQDV
                     ALTRGEFEVLALLVRHPGEILDRDRLSRAVSGHDWSPQDRSVDVLVRRLRAKLDDPAR
                     TDSLIATIRGEGYRLAADVEPGGV"
     gene            complement(5057..5141)
                     /locus_tag="PKV97_06545"
     tRNA            complement(5057..5141)
                     /locus_tag="PKV97_06545"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5105..5107),aa:Leu,seq:tag)
     gene            complement(5220..6668)
                     /locus_tag="PKV97_06550"
     CDS             complement(5220..6668)
                     /locus_tag="PKV97_06550"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNB05573.1"
                     /translation="MTIKSVILSGGSGTRLWPASRESYPKQLLPLTGERSLLQETALR
                     LKDFPGGEVDPRPLVVTNEEYRFIIAEQLRQIGVRSPQIVLEPMGRNTAPALTLAALV
                     AVEEGDPILLVMPADHVITEQVAFQHAIAIGAKAAAKGALVTFGIVPDRPETGYGYLR
                     SGEALAEGEGARALLEFVEKPDRATAEAYVASGAYYWNSGIFMMKASVWLAAIRRFAP
                     EMATACTTAVTGCTHDADFIRLDTAAFERSPSDSIDYAVMEKLAEAPELGQGVVVPLS
                     AGWSDVGAWDALWAVSAKDGEGNAARGEVLFEACRNTLVHAGSRLVAAVGCEDMVVVE
                     TPDAVMVAHKDRTQDVKKVVARLKAEGRTLTQSHRKVYRPWGWYDSIDAGERFQVKRI
                     VVNPGAKLSLQMHHHRAEHWIVVKGTAEVTNGERSFLLAENESTYIPLGHTHRLSNPG
                     KVPLEIIEVQSGSYLGEDDIVRFEDTYGRSTA"
     gene            complement(6710..7600)
                     /gene="rfbD"
                     /locus_tag="PKV97_06555"
     CDS             complement(6710..7600)
                     /gene="rfbD"
                     /locus_tag="PKV97_06555"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNB05574.1"
                     /translation="MKILLLGKNGQVGWELQRALAPVGTVVALDRGGADGLHGDLEDL
                     DGIDRTVRSLAPDVIVNAAAYTAVDKAETDVERAQRINAEAPGVLARAAATVGALLVH
                     YSTDYVFDGSGDTPWRENAPTAPLSVYGRSKLAGEEAIRAAGCRHLILRTSWVYAARG
                     GNFARTMLRLAAERERLTVIADQHGAPTGAELIADVSAHALRAEHADRSLGGTYHLAA
                     GGETSWHGYASFVIEQARKLGATLKAGEIAPIGTRDYPTAAARPLNSRLDTSRLRERF
                     GLALPDWRDGVARMLREILD"
     gene            complement(7597..8667)
                     /gene="rfbB"
                     /locus_tag="PKV97_06560"
     CDS             complement(7597..8667)
                     /gene="rfbB"
                     /locus_tag="PKV97_06560"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNB05575.1"
                     /translation="MILVTGGAGFIGANFVLDWLQGSDEPVINLDALTYAGNRETLAR
                     LEGDARHLFVHGDICDRALVERLFAEYKPRAVVHFAAESHVDRSIHGPGAFVRTNVDG
                     TFTLLEAARAHWSALPAGEREAFRFLHVSTDEVYGSLGPNDPAFTETKAYEPNSPYSA
                     SKAASDHLVRAWHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIVNALAGKPLPIYGDG
                     QNVRDWLYVGDHCSAIREVLARGRLGETYNVGGWNEMANLEIVHTLCALLDELRPSAA
                     GRHARLITYVKDRPGHDRRYAIDARKIERELGWRPAETFQSGIRKTVRWYLDNPAWIA
                     NVQSGAYRDWVARNYAGREARA"
     gene            complement(8792..>9535)
                     /locus_tag="PKV97_06565"
     CDS             complement(8792..>9535)
                     /locus_tag="PKV97_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05576.1"
                     /translation="VETVAMLHAFPADQELPWIMPKNSKPTRYNRLVMGMLLSFLATG
                     HSQANDHAAGRTLRPTAVALHIHEEIADQRFVPKLVARLQGSLAPPIVTIPTKMDLAP
                     FRPTLGLMDARPLLDALIGSIDWRGDGTVVRVVVIADDMQLKPARFNFAASAGDTSTP
                     FHLSIVSLARLQQLTDRGRVDRDPDRTAQRAFKLIAKNTARLMGYASSDACLFAFPRS
                     LSELDATPENFCEPDLSALVAAGIARSPQ"
BASE COUNT         1409 a         3321 c         3377 g         1428 t
ORIGIN      
        1 ggcggcaaca tccactgcac cgggaaacgt cccagcgagc tcttgtccac gagttcgacc
       61 gccgggccga ggtagggctc gatcggccgc ccctccagcg cccgcaaggc ctgacgcacc
      121 gccagccgcc cctgcagcac cgggaaatcc gtcaccgccg cgaggatgcg gccgcgcagg
      181 atgccgccat ggacggccgg agtcaggtag gacgacacga tcgccgtcgc cccctcgcgt
      241 ccgcgccggc gcagctcggc gatcgcggcc tcggccatgg gcgcgttgcc gaccaggtaa
      301 tcgagctgcg gctgctcgtc gagcacagcc tccaccagcc ggcgctggat cgcgcgcccc
      361 gtgtcaccgt ggcgctcggc gacgacgttc accgcgctgc ctgcgatgcc cgtgcggaaa
      421 ccgcggtcga tgaagcccac ccagtccgcg tccgccggtc cgggaatcca cgcgatccgc
      481 gcggggggac cgcccgcggg atggcgtgcg gccagccagc ggccgatcgt ccagccgagc
      541 tggtaccagg gcacggcgat gcgcgcgcgc accacctgcg ggtcgatgtc gttcaccacc
      601 ccgagcacga gccgatggtc gagttgcggc gcgagcaggt cgttgagccc gctgcggctg
      661 acggtgccga tcagcagcgc atcgaccgcc gcatcctcca ggcactccgc cacgcgttcg
      721 cgctggcggg cgagctgccc gtagccgccg gcctcgcgta cgcccaggcc gaggccgagc
      781 gcgcgcgcct cctcggtcat gccgaaattc accgccagcc agtagctgtc cttcaggtgc
      841 gggtagatcg cgcacaggcg ccagccacgc gtgggggagg gcggaacgat gtccggcccg
      901 gtctcggccg cgccgtttgc cgcgcgatga tcgcctgccc agcgttgcag cggccactcg
      961 gctgccggct gcgcaccggc cagcgccgcg gcgcagctcg gcagcgcgcc cagcgcaagc
     1021 ccgagcaaac cgtggcggac gctgcgccgg taagatgaaa cccatcgact cgtcgccttc
     1081 gaccactgcg caagcacacc cgctccccac cgaccgtgac cgccccatct tacgtgaaac
     1141 cccgccacga acgccgcttc ggcctgcgcg aacgcctgct gctggggctg ctcgtcgccg
     1201 cgctcggcac cgtactggtg gcggtggtgg gctgggtcag cttccagcgc gtggtgagca
     1261 gccagcaggc gatcgtgcgc gacaccctgc cggccgccga cgcgctgcac gaggcggtgc
     1321 gcgccaacgc ccgcctcgcg gcgcttgcgc cgcggctgct gcgggcggat tcggcggccg
     1381 agctcgatca gttgcgcgcc gcgctcggtg tcgacgtgcc gttgctgcgc gaccgcctgg
     1441 cggcgctgcg ctcgccgcac gtcgaggcgg agctgcgcga gcgcctgctg gcgaccggcg
     1501 accgcctcgc cgccggcctc gaaggcatga gcgcggcgat cgaccaacgc ctgcgcctgc
     1561 gtgcagcccg cctcgagcgc atcgacacgc tgcgcgcggc gatcgacggg ctcgacgagc
     1621 ttgcccgcat ccacgcggat aacgccaccg cgcagctcgt gtccaccctc acgaccctgc
     1681 tgcagcccga ccccgggcag ggcgcgccga gcttggccga gcgcgaggct gcgcgcgagc
     1741 gtgtgctcga tctcgacatc gatagcctcg aacgcatgca cgaactcaac ctcaccgtgc
     1801 atgcgctcgg ctttctcatc ggccgcctcg acgagctcga ctcgggcgca cggctgcagg
     1861 cggcacgcgt cgagttcgcc gcgcacctcg cactgctggc gcgccggctc gcggatatcg
     1921 ccgacccggg tgggcgggag cagggcgagc gggtgctccg attgctcgcc tcggcgctgg
     1981 acgaggaggg tacgttcgca ctgcgcgcac gcgagatcgc cctgcgcgcg caggtggaga
     2041 ccttgcaggg cgtggtcggc gaactcacca ccgagctcga cgccctcgcc ggcgagctga
     2101 tccaccgcgg cggccgcatc ctcgccgccg ccggatcggc cgccgaacgc agcgcgacct
     2161 cgggtctgat cgccttcggc gtgatcgccg cggcgctgct gctggtgacc ctgggcgtca
     2221 ccgtccatgc gctgcggcgt cacacgctcg ggcgcctgcg cgcgctggaa gaggccaccc
     2281 tcgcactcgc gtccggccga cgcgaggtgg cgatcgacac cgcgggcgac gacgagctgg
     2341 cctcgctcgc ggtcgcgctc gaacgcttcc gcgccaacgc gatcgagcgc gaccgcttcg
     2401 cagaagagct tcggcagcag cagcaggagc tcgagaatca ggtgctggcg cgcaccgcgc
     2461 agctgcgcga ggccaacgcc gcgctcgcgc gcgagaccgc cgaccacgct cgcgcccgtc
     2521 acgccgccga gcaggccgac cgcgccaaga ccgccttcct cggcaccgtc agccacgagc
     2581 tgcgcacccc gctcgcgggt atcctcggtc tgctcgaact cgtcgaggac gccagctcga
     2641 ccaccgagcg caagcagtac caggcgcaga tgcgcgccgc ggccgtgctg ctgctcgagc
     2701 tgctcgagga catgctcgac ttcgcccgca tcgaagcggg cggcgtgcag gtcgacaagg
     2761 cgagcttcag cctgcgcgac acggtgaacg atgtcttcgc ggtgcagggc acgcgcgccg
     2821 cggtacgcgg gctcgcgctg atcgccgagg tcgatcccgc gctgcccgac gcggtgcagg
     2881 gcgaccgccg caagctcagc cagatcctgc tcaacctggt cggcaacgcg atcaaattca
     2941 gcgacgcggg cgcggtgacg gtgcgcgtgc gcgccggtac gcaacccgac cagctgcact
     3001 tcgccgtcga ggaccacggc atcggcatcg agcccgcgcg ccagcaggag gtcttcgagc
     3061 ccttcgtgca ggtgcgcgac agcggccgcc accacgccgg caccggcctc gggctggcgg
     3121 tgtgccgccg cctggtcgag gccatgggcg gtgcgatcga gctgaagagc gcgccagggc
     3181 agggcaccac ggtgagtttc gagatccccc tcccggcgac cgcagcggcg gccgtcgagg
     3241 ccttgcctgc cgcgacggag ccggagcggg ccgcgctgcc ccccggccac cgcgtgctgg
     3301 tggtcgagga tgacgaggtc aatcgcatgg tggtcgagcg cttcctcgac gccctcggtc
     3361 agcaggcggt gtgtgcgccc gacatccaga acgcactgcg cctgctgcag gcgcgcccga
     3421 tcgacctcgc cctgatcgac atgaacctgc ccgacggcga cggccgcgag ctgctcgccc
     3481 gcctgcgcgc gctgccggcg cacgcgcaca ctccggccgt gctgatgtcg gcccacatcc
     3541 cgcgccgaga ggtcgatgcg ctgctcgaag cgggcttcgc cgccttcctg tccaagccct
     3601 tcgcgcgcga gcgcctgcgc gcgctgctcg ccgatctcct tgccgagcca gcggccgcag
     3661 cacccacgca aggcacggcg ggtgtcgcgg aggtcctgca ggcgacagac tggatcgacg
     3721 ccgatttcct gcgtgcggag cgggaggcgc tgggcgagga gaccgtggcc gacatcgtcg
     3781 gcgtctttcg cacgcagggc cagccgctga tcgacgcgct gctcgccgcc gcccgcgccg
     3841 gcgagcacga agcctgcgca cgcctcgcgc acaagctgcg cggcgccgcg ggcaacgtgg
     3901 gggcagggcg gctggcggaa tgcgcgggcg cgctggagga ggcgctgaaa ccggacccga
     3961 agggggacgc gccgcgcgcg ggcgcgatcg gcgacctggc cgcgcgcgcg ggcgagctgg
     4021 gcgaggcctg gtcgtacacg ctgcaggcgc tcgacgcctt gcgttctgcg gcgaacgaca
     4081 ccgaagcgca aaggccggat cagaccccgc caggctcgac gtcggcagcc agccgatagc
     4141 cctcgccgcg gatcgttgcg atcagcgagt ccgtacgcgc cggatcgtcg agcttcgcgc
     4201 gcaggcggcg caccagcaca tccaccgaac ggtcctgcgg cgaccagtcg tgcccgctca
     4261 ccgcgcggct gaggcgatcg cggtcgagga tctcgccggg gtggcgcacc agcagcgcga
     4321 gcacttcgaa ttcgccgcgc gtcagcgcca cgtcctgccc cgcgcggtcg cgcagcctgc
     4381 gccgcgaaag ttccagcacc cagcccgcga agcgcaggct gtccgccgtc tccgccggac
     4441 gcgtcgcgct gcgccgcagc aggctcttga cgcgggcgag cagctcgcgc gggttgaagg
     4501 gcttggtcac gtagtcgtcg gcgcccacct cgagcccgac gatcttgtcg acgtcgtcgt
     4561 tgcggctggt gacgaggatg atgccgatcg aggcgtcgcg cgcgcgcagc tcgcgcgcca
     4621 acgacaagcc gtcctgtccg ggcaggttga tgtcgagcag cacgaggtcg acctctccct
     4681 gggcgagctg tcgccagaag gactcgccgt cgccggccag cagcacgcgg tagccttcct
     4741 ttgaaaagta ggcattcatg cgcgcctgcg cgacgagatc gtcgtcgacg accaagaggg
     4801 tgacgtggga ttcgaggggc gcttgcatgg gatcgcgagg gacgcgaggg gtgggaggca
     4861 ggaaggagcg aggaggacag gcgggattct aaggctggaa ggcgaattct tcgcaccgag
     4921 atgccatgtc ggatagttgt ttacataaca catattctcg ctgttttagt tgcacctact
     4981 gcagggggac ttcctggccg cgagcgcgct tgcgggaacg ctgcagcacg cgcgtgcaat
     5041 gccagattga aaaccctggt gcggggaaag agactcgaac tctcacgcct tgcggcgctg
     5101 gaacctaaat ccagtgcgtc taccaattcc gccatccccg cacacttcag cacaagtgaa
     5161 gcggcgcgca ttatacacgc gccgcttcgc gtaccgggcg gcagcggctg gtccgacgct
     5221 caagccgtgc tgcggccgta ggtgtcctcg aagcggacga tgtcgtcctc gccgaggtag
     5281 ctgcccgact gcacctcgat gatctcgagc gggaccttgc ccgggttcga caggcggtgg
     5341 gtgtggccga gcgggatgta ggtcgattcg ttctcggcga gcaggaagct gcgctcgccg
     5401 ttggtgacct ctgccgtacc cttcaccacg atccagtgct cggcgcggtg gtggtgcatc
     5461 tgcaggctga gcttggcgcc cggattgacc acgatgcgct tgacctggaa gcgctcgccg
     5521 gcgtcgatcg agtcgtacca gccccaggga cgatagacct tgcgatggct ctgggtgagc
     5581 gtgcggccct cggccttcag gcgcgcgacc accttcttga cgtcctgcgt gcgatccttg
     5641 tgcgcgacca tcaccgcgtc gggggtctcg accaccacca tgtcctcgca gcccaccgcg
     5701 gcgaccaggc ggctgccggc gtgcaccagg gtgttgcggc aggcctcgaa gaggacctcg
     5761 ccgcgcgcgg cgttaccctc cccgtccttg gccgacaccg cccacagcgc gtcccaggcg
     5821 cccacgtcgg accacccggc cgacagcggc acgaccacgc cctgcccgag ctccggcgct
     5881 tcggcgagct tctccatgac tgcgtagtcg atcgagtcgc tcgggctgcg ctcgaaggcg
     5941 gcggtgtcga gacggatgaa gtccgcgtcg tgggtgcagc cggtgaccgc cgtcgtgcag
     6001 gccgtggcca tctccggcgc gaagcgacgg atcgccgcca accacaccga ggccttcatc
     6061 atgaagatgc cgctgttcca gtagtaggcg ccgctcgcca cgtaggcctc ggcggtggcg
     6121 cggtcgggtt tctcgacgaa ctcgagcagt gcgcgcgcgc cctcgccctc cgccagcgcc
     6181 tcgccgctgc ggaggtagcc gtagccggtc tcggggcggt cgggcacgat gccgaaggtc
     6241 accagcgcac ccttggctgc cgccttggcg ccgatcgcga tcgcatgctg gaaagcgacc
     6301 tgttcggtga tgacgtggtc ggcaggcatc accagcagga tcggatcgcc ctcctccacc
     6361 gcaaccagcg cggccaaagt gagcgccggc gcggtgttgc gtcccatcgg ctcgagtacg
     6421 atctgtggcg aacgtacgcc gatctggcgg agttgttcgg cgatgatgaa gcgatactcc
     6481 tcgttggtga cgaccagcgg acgcgggtcg acctcgccgc cggggaagtc cttcaggcgc
     6541 agcgcggtct cctgcagcag cgagcgctcg ccggtaaggg ggaggagttg cttgggatag
     6601 ctctcgcgcg acgccggcca cagtcgggtg ccggagccgc cggagaggat cacgctcttg
     6661 atggtcatga atcgggtcct tgcgttgttc tgtcgttcgg ccgggtgctt cagtccagga
     6721 tctcgcgcag catgcgggcg acgccgtcgc gccagtcggg cagcgcgaga ccgaagcgtt
     6781 cgcgcagccg cgaggtgtcg agccgtgaat tgagcggccg cgccgcggcg gtcggatagt
     6841 cgcgggtgcc gatcggcgcg atctcgcctg ccttgagcgt cgccccgagc ttgcgcgctt
     6901 gctcgatcac gaagcttgcg tagccatgcc agctcgtctc gccgcccgcg gcgaggtgat
     6961 aggtgccgcc caggctgcgg tccgcgtgtt cggcacggag ggcgtgcgcg ctgacgtcgg
     7021 cgatcagctc cgcgccggtg ggtgcgccgt gctggtcggc gatgacggtc aggcgttcgc
     7081 gctcggccgc gaggcgcagc atcgtgcgcg cgaagttgcc gccgcgcgcc gcatacaccc
     7141 aggaggtgcg caggatcagg tgacggcagc ccgcggcgcg gatcgcctcc tccccggcga
     7201 gcttgctgcg gccgtacacg gaaagcggtg cggtcggggc attctcgcgc cagggggtgt
     7261 caccgctgcc gtcgaagacg tagtcggtcg agtaatgcac cagcagcgcg cccacggtcg
     7321 ccgcggcccg ggccagcacg cccggcgcct ccgcgttgat gcgctgcgcg cgctcgacat
     7381 cggtctcggc cttgtcgaca gcggtgtagg cggcggcgtt gacgatcacg tcgggggcca
     7441 ggctgcgcac ggtgcggtcg atgccatcga ggtcctcgag gtcgccatgc aggccgtcgg
     7501 cgccgccacg gtcgagcgcg acgacggtgc cgaccggcgc gagcgcgcgc tgcagctccc
     7561 agccgacctg gccgttcttg ccgagcagca ggatcttcat gcgcgggcct cgcgtccggc
     7621 gtagttgcgc gccacccagt cgcgataggc gccgctctgc acgttggcga tccaggccgg
     7681 gttgtccagg taccaccgca cggtcttgcg gatgccgctc tggaaggtct ccgccggccg
     7741 ccagccgagc tcgcgttcga tcttgcgcgc gtcgatcgcg tagcggcgat cgtggccggg
     7801 gcgatccttg acataggtga tcaggcgggc gtggcggccg gctgcactcg ggcgcagctc
     7861 gtcgagcagc gcgcacaggg tgtgcacgat ctcgaggttg gccatctcgt tccatccgcc
     7921 gacgttgtag gtctcgccga ggcggccgcg cgccagcact tcgcggatcg cgctgcagtg
     7981 atcgcccacg tagagccagt cgcgcacgtt ctggccgtcg ccgtagatcg gcagcggctt
     8041 gccggcgagc gcgttcacga tcatcagcgg gatcagcttc tcggggaact ggtaggggcc
     8101 gtagttgttc gagcagttgg tggtgagcac cggcaggccg taggtgtgat gccaggcgcg
     8161 caccaggtgg tcgctcgccg ccttgctcgc cgagtagggg ctgttgggct cgtaggcctt
     8221 ggtctcggtg aaggcgggat cgttcgggcc gagcgagccg tagacctcgt cggtgctcac
     8281 gtgcaggaag cggaaggcct cgcgctcgcc ggcgggaagc gcgctccagt gcgcgcgcgc
     8341 cgcttcgagc agggtgaagg tgccgtctac gttggtgcgc acgaaggcgc cgggaccgtg
     8401 gatggagcgg tcgacgtggc tctccgcggc gaagtgcacc accgcgcgcg gcttgtattc
     8461 ggcgaacagg cgctcgacca gggcgcggtc gcagatgtcg ccatgcacga agaggtgccg
     8521 cgcgtcgcct tccaggcgcg cgagcgtctc gcggttgccg gcataggtca gcgcgtcgag
     8581 gttgatcacc ggctcgtccg aaccctggag ccagtcgagc acgaagttcg cgccgatgaa
     8641 gccggcgccg cctgtcacga gaatcacgtg gggaatcctg ttcgagtctg gaaagctatc
     8701 attatcgcat tcagggcaat ggccgacgag gaaggacgcc actccggatc acaggttttg
     8761 agtctcgctc caccgctgcg tcctcgggca atcactgcgg cgatcgagcg atgcccgccg
     8821 cgacaagggc cgagagatcc ggctcgcaga agttctcagg cgtggcatcg agttcggaaa
     8881 ggctgcgggg aaaggcgaaa aggcaggcat cgctcgacgc gtatcccatg agtcgggcgg
     8941 tgttcttggc gatcagcttg aacgcgcgtt gcgcggtgcg gtcggggtcg cgatccacgc
     9001 gcccacggtc ggtgagttgc tgcagccttg caagcgaaac gatcgaaagg tgaaacggcg
     9061 tactcgtgtc tcccgcactg gcggcaaagt tgaaacgcgc cggtttcaat tgcatgtcgt
     9121 cggcgatcac gacgacgcga acgaccgttc catcccctcg ccagtcgatg ctgccgatca
     9181 atgcatcgag gagcggacgc gcatccatga gcccgagcgt tggccgaaaa ggcgcgagat
     9241 ccatcttggt cgggatcgtc acgatcggtg gtgcaaggct gccttgcagg cgcgcgacca
     9301 gtttcggaac aaagcgctgg tcggcgatct cctcgtggat gtgaagggct accgccgtcg
     9361 gacgcaaggt ccggccggca gcatggtcgt tggcctggct gtggccggta gccagaaacg
     9421 acagcaacat gcccatcaca agtcggttgt accttgtggg ctttgagttc ttcggcataa
     9481 tccagggcag ctcctgatcg gcgggaaacg catgcagcat agctacagtt tcgac
//
LOCUS       DAOQNN010000084         9498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3482_length_9498_cov_10.383776, whole genome shotgun sequence.
ACCESSION   DAOQNN010000084 DAOQNN010000000
VERSION     DAOQNN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3482_length_9498_cov_10.383776"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="PKV97_06570"
     CDS             complement(<1..1591)
                     /locus_tag="PKV97_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB05577.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALLDNPSAAQVL
                     KACAANIDELRRELTGFIEEHTPRIEGTDEIDTQPTLGFQRVIQRAILHVQSSGKKEV
                     TGANVLVAIFGEKDSHAVYYLQKQNISRLDVVNFISHGIAKAPPQGNPPQGRSEPEQG
                     EQEQEEKSSGGALESYTQNLNQQALVGKIDPLIGREKEVERVIQTLCRRRKNNPLLVG
                     EAGVGKTAIAEGLARRIVEGRVPEILENAQVFALDMGALLAGTKYRGDFEQRLKAVLK
                     QLIENPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGQLKCIGATTYNEFRQI
                     FEKDHALSRRFQKIDVVEPSVAETVEILKGLKSRFEEHHGIKYSSSALSSAAELSAKY
                     INDRHLPDKAIDVIDEAGAAQRILPKSKQKKTIGKNEIEEIVAKIARIPPRTVSNDDK
                     AALKTLERDLKNVVFGQNAAIEALAKAIKMSRSGLGNPQKPIGSFLFSGPTGVGKTEV
                     ARQLAYTLGIELVRFDMSEYME"
     gene            complement(1592..1900)
                     /gene="clpS"
                     /locus_tag="PKV97_06575"
     CDS             complement(1592..1900)
                     /gene="clpS"
                     /locus_tag="PKV97_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNB05578.1"
                     /translation="MGTRKQDDLVLEAKRTRTRPPPLFKVMLLNDDYTPMDFVIVVLQ
                     KYFGMDRERATRVMLQVHREGMGVCGVFPRDIATTKVEQVTSFARQHQHPLACVMEEN
                     "
     gene            2289..2492
                     /locus_tag="PKV97_06580"
     CDS             2289..2492
                     /locus_tag="PKV97_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNB05579.1"
                     /translation="MATGTVKWFNDSKGFGFITPDDGSEDLFAHFSAITMSGFKSLKE
                     GEKVSFDVTQGPKGKQASNIQRA"
     gene            complement(2634..2801)
                     /gene="rpmG"
                     /locus_tag="PKV97_06585"
     CDS             complement(2634..2801)
                     /gene="rpmG"
                     /locus_tag="PKV97_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNB05580.1"
                     /translation="MAKGIREKIKLESTAGTGHFYTTSKNKRTTPGKLEFSKYDPVAR
                     KHVPYKEVKLK"
     gene            complement(2827..3063)
                     /gene="rpmB"
                     /locus_tag="PKV97_06590"
     CDS             complement(2827..3063)
                     /gene="rpmB"
                     /locus_tag="PKV97_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNB05581.1"
                     /translation="MARVCQVTGKAPMVGNNVSHANNKTKRRFLPNLQSRRFWSEGEN
                     RWIRLRVSNAGLRTIDKKGIDVVVAELRARGEKL"
     gene            complement(3211..3888)
                     /gene="radC"
                     /locus_tag="PKV97_06595"
     CDS             complement(3211..3888)
                     /gene="radC"
                     /locus_tag="PKV97_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNB05582.1"
                     /translation="MAITDWPADERPREKLLARGAAALSDAELLAIFLRVGIRGQSAV
                     DLARALLSRFGTLTRMCGADANEFAAVPGMGLAKYAQLQAVMELSRRALAETLAARPL
                     FDSPQVVRDWLRLRLGHLPHETFCVLLLDARHCLIEAVDLFRGTLTQTSVYPREVVKL
                     ALARNAAAVILAHNHPSGVAEPSTADELLTRALKDALGLVDVRVLDHFVVPAHGHPVS
                     FAERGLL"
     gene            3967..5163
                     /gene="coaBC"
                     /locus_tag="PKV97_06600"
     CDS             3967..5163
                     /gene="coaBC"
                     /locus_tag="PKV97_06600"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNB05583.1"
                     /translation="MSMLSNRKIVLGVGGGIAAYKSAELVRLLGKAGAEVHVVLSEGG
                     ARFVTPVTYQALSGNPVWSDLWDARMPNHMAHIDLGRDADAILVAPATADFLARLAHG
                     HADDLLSTLCLARAVPLIVAPAMNRQMWESPPTQRNVAQIRADGVAVFGPAAGDQACG
                     EVGMGRMLEAEELLERLEGFFVPKLLAGRKVVLTAGPTFEALDPVRGITNISSGKMGY
                     ALARACAQAGAEVVLVSGPVSLPVPAEVTRVGVTSALEMRDAVSAALAGADVFIGVAA
                     VADYRPLASAEHKLKKSADTMNIALTPNPDILAEVAAHADAPFCVGFAAESRDLDAYA
                     EGKRRNKRLPMLVGNLVSDGMGGDDNTVILYDDAGRHPLPRAAKAEVARGIVAHLAGL
                     LPARGR"
     gene            5223..5672
                     /gene="dut"
                     /locus_tag="PKV97_06605"
     CDS             5223..5672
                     /gene="dut"
                     /locus_tag="PKV97_06605"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNB05584.1"
                     /translation="MHRIDVKLLDERLKTHPPAYATEGAAGLDLRACLPGPITLHPGE
                     TTLVPSGLAIHLSDPGLAAMVLPRSGLGHKHGIVLGNLIGLIDSDYQGQIFVSVWNRG
                     RASFTIEPMERIAQLVVVPVLQVGFNFVEGFEQSSRGADGFGSTGKH"
     gene            5672..6112
                     /locus_tag="PKV97_06610"
     CDS             5672..6112
                     /locus_tag="PKV97_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB05585.1"
                     /translation="MHAGIPTGVHVLCRREGRVLLMRRAGTGFFDGLFSLPGGHVEPG
                     ESVRAAARRELREETGLEVEEGELAWLGVVHRRSDTNRIDFFLAAERFSGEPAILEPH
                     KCDRLEWHAPGALPERTVEYVRAALAAGPAPWILELGWKEAGTA"
     gene            6352..6816
                     /gene="soxY"
                     /locus_tag="PKV97_06615"
     CDS             6352..6816
                     /gene="soxY"
                     /locus_tag="PKV97_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HNB05586.1"
                     /translation="MDQKRRQLLHAVGGLGVLGLLATAGLISPAQAQAAAARPGFDAR
                     ELNAALAALGAQEASASAELSLAAPDIAEVANAVSVEVKSRLPGTDRIAILVEKNPFP
                     LAAVFSIPEGTQPEVITRLKMAQSADVVALVRANGRFHIARKAVIVTISGCG"
     gene            6844..7167
                     /gene="soxZ"
                     /locus_tag="PKV97_06620"
     CDS             6844..7167
                     /gene="soxZ"
                     /locus_tag="PKV97_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HNB05587.1"
                     /translation="MTNPMRIRADVRDGVADVRMQVPHPMESGQRKDDKGAIIPAHFI
                     TRLEVHHGERLVFGADFGPSVSANPFLRFRFKGAAVGDRLVARWVDSRGDSRSDEAVI
                     AGAPA"
     gene            complement(7198..8967)
                     /gene="aceK"
                     /locus_tag="PKV97_06625"
     CDS             complement(7198..8967)
                     /gene="aceK"
                     /locus_tag="PKV97_06625"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HNB05588.1"
                     /translation="MDVQSGESPIAQAIAQALVEGFNKHYRIFRETSRRAKELYEAAL
                     WQKQLDAVRERVQFYDDRVDETVQRLHDEFDADSLDDSTWQQVKLQFIGMLINHRQPE
                     LAETFFNSVTTKILHRSYFNNDYLFARPAISTEYIESYPPVYSSYYPRDRGLRATVRR
                     IVEDFDWQRSFEDLDRDVDFVLRAARAHLGEWPAMEVNCQIQVLYSAFYRNKTAYIIG
                     KTVNGYQESPFAIAVRHNERGLLHLDALILDPWRISVLFSLSRAYFLVDMEVPSGYVQ
                     FLRSIMPNKPRSELYTMLGLGKQGKTMFFRDLVSHLRHSNDQFIIAPGIRGLVMLVFT
                     LPSFPYVFKIIKDVFGASKNMDRATVKRKYLMVKHVDRVGRMADTLEFSYAALPLARF
                     HPELLEELRTLAPSSFEIDGDKVIIKHLYIERRMIPLNLHLEKASEPELEEAVKEYGN
                     AIRELAIANIFPGDMLWKNFGVTRYGRVVFYDYDEIEYMTDMNFRKIPPAPYEEMEMS
                     GEPWYSAGPMDVFPEEFATFLLGVPRVRKAFLKHHRDLLAPKFWQEAQRRIREGYVED
                     FFPYPVELRFCKLFPPAAGAASS"
     gene            9256..>9498
                     /locus_tag="PKV97_06630"
     CDS             9256..>9498
                     /locus_tag="PKV97_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNB05589.1"
                     /translation="MSESKIKVPAGGQKIVPGQAVPDNPIIPFIEGDGIGVDITPVMI
                     KVIDAAVDKAYGGKKKIHWMEVYAGEKSTQIYGPDEW"
BASE COUNT         1525 a         3048 c         3274 g         1651 t
ORIGIN      
        1 gttccatgta ttcggacatg tcgaagcgca ccagctcgat gcccagcgtg taggcgagct
       61 ggcgggcgac ctcggtcttt ccgacgccgg tggggccgga gaacaggaag gagccgatcg
      121 gcttctgcgg gttgccgagc ccggagcgcg acatcttgat cgccttggca agggcctcga
      181 tcgcggcatt ctggccgaac accacgttct tgagatcgcg ctcgagcgtc ttcagcgcgg
      241 ccttgtcgtc gttggacacg gtgcgcggcg ggatgcgggc gatcttggca acgatctcct
      301 cgatctcgtt cttgccgatg gtcttcttct gcttcgactt gggcaggatg cgctgggctg
      361 cgccggcctc gtcgatgacg tcgatcgcct tgtcgggcag gtggcggtcg ttgatgtact
      421 tggccgacag ctccgccgcg ctggagagtg cagagctgct gtacttgatg ccgtggtgct
      481 cctcgaagcg gctcttgagc cccttgagga tctcgacggt ctcggccacc gagggctcca
      541 ccacgtcgat cttctggaag cgcctcgaga gcgcgtggtc cttctcgaaa atctggcgaa
      601 actcgttgta ggtggtcgcg ccgatgcact tcaactggcc cgaggacagc gccggcttga
      661 gcaggttgga cgcatccagc gtgccgcccg aggccgcacc ggcgccgatc agggtgtgga
      721 tctcgtcgat gaagaggatc gcgttcgggt tctcgatgag ctgcttgagc acggccttga
      781 ggcgctgctc gaaatcgccg cggtacttgg tgccggcgag cagcgcaccc atgtcgagcg
      841 cgaacacctg cgcgttctcg aggatctcgg gcacgcggcc ctcgacgatg cgacgcgcaa
      901 ggccttcggc aatcgcggtc ttgcccacgc cggcctcgcc gaccagcagc gggttgttct
      961 tgcgccggcg gcacagggtc tggatgacac gctcgacctc cttctcgcgg ccgatcaacg
     1021 ggtcgatctt gccgaccagc gcctgctggt tgaggttctg cgtgtagctc tccagcgcac
     1081 cgccggagga cttctcttcc tgctcctgct cgccctgctc gggttcgctg cgaccctgcg
     1141 gcgggttgcc ctgaggcggc gccttggcga tgccgtggga gatgaagttc accacgtcga
     1201 gacgcgagat gttctgcttc tgcaggtaat acaccgcgtg cgagtccttc tcgccgaaga
     1261 tcgccaccag cacgttggcg ccggtgacct ccttcttgcc cgaggactgc acgtgcagga
     1321 tcgcgcgctg gatcacgcgc tggaagccca gcgtgggctg ggtgtcgatc tcgtccgtgc
     1381 cttcgatgcg cggggtgtgt tcttcgatga aaccggtcag ctcgcgacgc aactcgtcga
     1441 tgttggcggc acacgccttc agcacctggg cggcggaagg gttgtccagc agggcaagca
     1501 gaagatgctc cacggtgatg aactcgtggc gcttctgccg tgcctcgacg aaagccatgt
     1561 gcaggctgac ttcaagctct tgcgcgatca ttcaattttc ctccatcacg catgccagcg
     1621 gatgttgatg ctgacgagca aacgaggtga cctgctccac cttggtcgtg gcgatgtccc
     1681 tgggaaagac gccgcatacc cccatgccct ctctgtggac ttggagcatg actcgcgtgg
     1741 cacgttcgcg atccatgccg aaatatttct gcaggacgac gatgacgaaa tccatcggcg
     1801 tgtagtcgtc gttcaacagc atgaccttga agagcggggg cgggcgcgtg cgcgtccgct
     1861 tggcttccag gaccagatcg tcttgttttc gggtgcccat aaaatcattc taatgaagtc
     1921 gaagcagagt gcaacacgcg cttcgtgtgc gcgtcgagtc ccctgaaaac cccaggcgga
     1981 gtgtggtttg gcctgcacca cgtgaatcgg gcttcttgcg ctgcggcatg aagccatctc
     2041 cacgccaggc gcgaaattgc gcctggaatg gaggtggatc cgggggcttt catgttgcgc
     2101 tgcgcgatcg aaagcccgcg aaatcccgtt gacgctctcg tgccgaatgc gtaaaaacga
     2161 aggcgtgcgc cggctcgagc ctgatcccga ggggcttccg gaggtggttt tggtccctgt
     2221 cagcgcccac acgaccgcgg tttgcgaagg ccgcggtgta acaagcaacc ttgaaggatc
     2281 caagcaatat ggcaactggt accgtgaagt ggttcaacga ttccaagggt ttcggtttca
     2341 tcacgccgga tgatggaagt gaagacctgt tcgcacattt ctccgccatc acgatgagcg
     2401 gtttcaagag cctgaaggaa ggcgagaagg tctccttcga cgtcacccag ggtcccaagg
     2461 gcaaacaggc ctcgaacatc cagcgcgcgt gattccgcgg cgcatgtgat cgtgcccgcc
     2521 actgccggtg gcgggtgaaa aaacagaagg gccatcggat cttccgatgg cccttccggt
     2581 tttctggagc tgccgcgttc tgccgggcgg ttgcgcgcaa ccgccgcgcc aaatcacttc
     2641 agcttcactt ccttgtacgg cacgtgcttg cgcgcgaccg ggtcgtactt gctgaactcg
     2701 agcttcccgg gtgtggtgcg cttgttcttc gaggtggtgt agaagtgacc cgtaccggcg
     2761 gtcgactcca gtttgatctt ttcgcggatg cccttggcca tggccttctc cgttcagttc
     2821 ggttgcttac agcttctcgc cacgggcgcg aagctcggca acgacgacgt cgatgccttt
     2881 cttgtcgatc gtgcgcagac ccgcgttgga cacgcgcagg cggatccagc ggttctcgcc
     2941 ttcgctccag aagcggcggc tctgcaggtt gggcaggaaa cgacgcttgg tcttgttgtt
     3001 ggcgtgggaa acgttatttc ccaccatcgg tgctttaccg gtcacttggc agacgcgagc
     3061 catacgatgc tccagggaat tctgattgcg gaaaacgtgc gaggataatg caagaagcgc
     3121 ggtcgaggca agccccgcgt accttcgtcg tgttcgcagg gtggaatgcc aggcagggtc
     3181 cggggccgga gcggaagggc gggcggaacc tcaaagcagg ccgcgttcgg cgaatgatac
     3241 cggatgaccg tgcgccggca ccacgaaatg atcgagcacg cgcacgtcga ccaggccgag
     3301 ggcgtccttc agggcgcgcg tgagcagctc gtcggcggtg ctcggctcgg caacgcccga
     3361 ggggtggttg tgggcgagga tcacggccgc ggcgttgcgc gcgagggcga gcttgaccac
     3421 ctcgcgggga tacaccgagg tctgcgtgag cgtgccgcgg aacagatcga ccgcttcgat
     3481 caggcagtgc cgtgcgtcga gcagcagcac gcagaaggtc tcgtggggca ggtgtccgag
     3541 gcgcaggcgc agccagtcgc gtacgacctg gggtgaatcg aacagcgggc gtgcggccag
     3601 cgtctcggcg agcgcccgcc gcgacagttc catgaccgcc tgcagttggg catacttcgc
     3661 cagccccatg ccaggtaccg cggcgaactc gttcgcgtcg gcgccgcaca tgcgggtcag
     3721 ggtgccgaag cgggacagca gcgcgcgggc gaggtcgacc gcgctctgcc cgcggatgcc
     3781 cacgcgcagg aagatcgcca gcagctccgc gtcggacagc gccgcggcgc cgcgggcgag
     3841 gagcttctcg cgcggacgtt cgtccgcagg ccagtcggtg atcgccatcg cgtgtccgcc
     3901 tccctcgtcg tggcctgtcg gcctatccag aatgcatcga tccatcgcgg agtatagttc
     3961 atcgtcatga gcatgttgag caatcgcaag atcgtcctcg gggttggcgg tggcatcgcc
     4021 gcgtacaagt cggccgagct ggtgcgcctg ctcggcaagg ccggcgccga ggtgcacgtg
     4081 gtgttgagcg agggcggggc gcgcttcgtg accccggtga cctaccaggc gctgtcgggc
     4141 aatccggtgt ggtccgacct gtgggatgcg cgcatgccca accacatggc gcacatcgac
     4201 ctcggccgcg acgccgatgc catcctcgtc gccccggcga ccgcagattt cctcgcccgc
     4261 ctcgctcatg gccacgccga cgacctgctc agcacgcttt gcctggcgcg cgccgtgccg
     4321 ctgatcgtcg ccccggcgat gaaccgccag atgtgggaat cgccgccgac gcagcgcaac
     4381 gtcgcgcaga tccgcgccga cggcgtcgcc gtgttcggtc ccgcggcggg cgaccaggcc
     4441 tgcggcgagg tcggcatggg gcggatgctc gaggcggagg aactgctcga gcgcctagag
     4501 ggtttcttcg tgcccaagct gctcgcgggg cgcaaggtgg tgctcaccgc cgggccgacc
     4561 ttcgaggcgc tcgatccggt gcgcggcatc accaacatca gctcgggcaa gatgggttac
     4621 gcgctcgcgc gcgcctgcgc ccaggcgggg gccgaggtgg tgctggtgag cgggccggtg
     4681 agcctgccgg tgccggccga ggtgacacgc gtcggcgtga ccagcgcgct cgagatgcgc
     4741 gacgcggtgt ccgccgcgct ggcgggggcg gacgtcttca tcggcgtggc cgcggtggcg
     4801 gattaccggc cgctggcgag tgccgaacac aagctgaaaa agtccgccga taccatgaac
     4861 atcgcgctga cccccaaccc ggacatcctt gccgaggtgg ccgcacatgc cgacgcgcct
     4921 ttctgcgtcg gtttcgccgc cgagagccgc gacctcgacg cctacgccga gggcaagcga
     4981 cgcaacaagc ggctgccgat gctggtcggc aacctggtca gcgacggcat gggcggcgac
     5041 gacaacaccg tgatcctcta cgacgacgcc ggccgccacc cgctgccgcg tgcggccaag
     5101 gccgaggtgg cgcgcggcat cgtcgcccac ctcgccggcc tgctgccggc ccgcgggcgc
     5161 tgagcgcaaa cgcaacgaca ggctgggccg gcacgcgccg cccgccggca aggagagccg
     5221 acatgcaccg aatcgacgtc aagctgctcg acgagcgcct caagacccat ccccccgcct
     5281 acgccacgga gggcgccgcg gggctcgacc tgcgcgcctg tctgcccggc ccgatcaccc
     5341 tgcacccggg cgagaccacg ctggtgccga gtggcctcgc gatccatctt tccgacccgg
     5401 ggctggcggc gatggtgctg ccacgctccg ggctcggcca caagcacggc atcgttctcg
     5461 gcaacctaat cgggctgatc gactccgact accaggggca gatcttcgtc tcggtgtgga
     5521 accgtgggcg cgcgagcttc accatcgagc cgatggagcg catcgcccag ctcgtggtgg
     5581 tgccggtgct gcaggtcggc ttcaacttcg tcgagggctt cgagcagagc tcgcgcgggg
     5641 cggacggctt cggcagcaca ggcaagcact gatgcacgca gggattccta ccggcgtcca
     5701 tgtgctgtgc cggcgcgagg gccgcgtgct gctgatgcgc cgtgccggaa cgggtttctt
     5761 cgacggcctc ttcagcctgc ccgggggaca cgtggagccg ggcgagtcgg tgcgcgcggc
     5821 ggcgcggcgc gagctgcgcg aggagaccgg gctggaggtc gaggagggcg agctggcctg
     5881 gctgggtgtc gtccatcggc gctcggacac caatcgcatc gacttcttcc tcgccgcgga
     5941 gcgcttcagt ggcgagcccg ccatcctcga gccgcacaag tgcgatcgcc tcgaatggca
     6001 cgcgcccggg gccctgcccg agcgtacggt cgagtacgtg cgcgccgcgc tcgcggccgg
     6061 cccggcgccg tggatcctgg agctcggttg gaaggaggcg ggcaccgcgt agccgccacg
     6121 ctgtgcgcag gagcgacgcc atggcgagcg tggcggcggg cctcgcgccg gaggacgtgc
     6181 tcgcgctcgg cgtcgatttc gaccggaagc ccgccaagcc gcgcccgacg cgcgacggcg
     6241 tgctcgccgg atggacgatg ctgcgctgcg ccagagcggt cccgggtgtg cgagtattgc
     6301 cgcacgcggc cgccacgcgc agccgccatg cgaaccctgc aggagagccg gatggaccaa
     6361 aaacgaagac aattgctgca tgccgtcggg ggcttgggcg tgctgggcct gcttgccacc
     6421 gccggtctga tctcgcccgc gcaggcgcag gccgccgccg cgcggcccgg tttcgacgcc
     6481 cgcgagctga acgcggcgct cgccgcgctg ggggcgcagg aggccagtgc gagcgccgag
     6541 ctgagtctcg ccgcccccga catcgccgag gtcgccaacg ccgtctcggt cgaggtgaag
     6601 agccgcctgc ccggcaccga ccgcatcgcg atcctggtcg agaaaaatcc cttcccgctt
     6661 gccgcggtgt tctccatccc ggagggcacc cagcccgagg tgatcacccg gctgaaaatg
     6721 gcgcagagtg ccgacgtggt cgcgctggtg cgggcaaacg gccgcttcca catcgcccgc
     6781 aaggcggtca tcgtcaccat cagcggctgc ggctgagccg cgccggcaca ggagagcgac
     6841 tccatgacaa acccgatgcg catccgcgcg gacgtccgcg acggcgtcgc cgacgtccgc
     6901 atgcaggtcc ctcatccgat ggagagcggc cagcgcaagg acgacaaggg cgcgatcatc
     6961 cccgcgcact tcatcacccg gctggaggtc caccacggcg aacgcctcgt cttcggcgcc
     7021 gacttcggcc catccgtttc ggccaacccc ttcctgcgct tccgtttcaa gggggcggcc
     7081 gtgggcgatc gcctggtggc gcgctgggtc gatagccgcg gcgacagccg cagcgatgag
     7141 gcggtgatcg ccggcgcgcc ggcctgagcg cttgccggcc ggttcccggg cgggcgctca
     7201 gcttgaagct gcgcccgcgg ccggcggaaa gagcttgcag aagcgcagct cgaccgggta
     7261 ggggaagaag tcctcgacgt agccctcgcg gatgcgccgc tgcgcttcct gccagaactt
     7321 cggcgcgagc aggtcgcggt ggtgcttgag gaaggccttg cgcacccgcg gcacgccgag
     7381 caggaaggtg gcgaactcct ccgggaagac gtccatcggc ccggccgagt accacggctc
     7441 gcccgacatc tccatctcct cgtagggcgc gggcgggatc ttgcggaagt tcatgtcggt
     7501 catgtactcg atctcgtcgt agtcgtagaa cacgacgcga ccgtagcgcg tgacgccgaa
     7561 gttcttccac agcatgtcgc cggggaagat gttggcgatg gcgagctcgc ggatggcgtt
     7621 gccgtactcc ttgaccgcct cttccaactc cggttcgctc gccttttcca ggtggaggtt
     7681 gagcgggatc atgcggcgct cgatgtagag atgcttgatg atcaccttgt caccgtcgat
     7741 ctcgaacgag gacggtgcga gcgtgcgcag ctcttccagc agttcggggt ggaagcgcgc
     7801 cagcggcagc gccgcgtagg agaactccag tgtgtccgcc atgcgcccga cgcggtcgac
     7861 gtgcttgacc atcaggtact tgcgcttgac cgtggcgcgg tccatgttct tcgaggcgcc
     7921 gaaaacgtcc ttgatgatct tgaacacgta ggggaaggag ggcagcgtga acaccagcat
     7981 caccaggccg cggatgcccg gtgcgatgat gaactggtcg ttggagtggc gcaggtgcga
     8041 gaccaggtcg cggaagaaca tggtcttgcc ctgcttgccc aggccgagca tggtgtatag
     8101 ctcgctcctc ggcttgttgg gcatgatcga gcgcaggaac tgcacgtagc ccgagggcac
     8161 ctccatgtcg accaggaagt aggcgcgcga cagcgagaac agcaccgaga tccgccacgg
     8221 gtcgaggatc agcgcgtcga ggtggagcag gccgcgttcg ttgtggcgca ccgcgatcgc
     8281 gaacgggctt tcctggtagc cattgaccgt cttgccgatg atgtaggcgg tcttgttgcg
     8341 atagaaggcc gagtacagca cctggatctg gcagttgacc tccatcgccg gccactcgcc
     8401 gaggtgggcc cgcgccgcgc gcagcacgaa gtcgacgtcg cggtcgagat cctcgaagct
     8461 gcgctgccag tcgaagtcct cgacgatgcg ccggacggtg gcgcgcaggc cgcggtcgcg
     8521 cgggtagtag ctcgagtaca ccggcgggta ggactcgatg tattcggtgg agatcgccgg
     8581 gcgcgcgaac aggtagtcgt tgttgaagta gctgcggtgc aggatcttgg tggtgaccga
     8641 gttgaagaag gtctcggcga gctcgggctg gcggtggttg atcagcatgc cgatgaactg
     8701 cagcttgacc tgctgccagg tgctgtcgtc gagggagtcg gcgtcgaact cgtcgtgcag
     8761 tcgctgcacc gtctcgtcga cgcggtcgtc gtagaactgc acgcgctcgc gcaccgcgtc
     8821 gagctgcttc tgccacagcg cggcctcgta gagttccttg gcgcggcgcg aggtctcgcg
     8881 aaagatgcgg tagtgcttgt tgaagccttc caccagcgcc tgtgcgatcg cctgcgcgat
     8941 cgggctttct cccgactgca cgtccatcga acccctccgg aggatcccgc cggcgcagcc
     9001 gcggcggtgt cgaacatgtc ggaaatggta tcaccggccg tgtcccggtc ggcgcagcca
     9061 tgctgcgccg cttcctttcg acctgcggat ctttgggaca taatcggcct cggatagggg
     9121 gctgtgcggc gagatgcagc gtgtcccgca cgccgtcgca gccttccggt ccgtcgtccg
     9181 acggacatcc ccgtggcagt tcgaccacag cagcgtcgtg gaatccatcc gcggccattg
     9241 agggagggag caggcatgag cgaatccaag atcaaggtac ccgcaggcgg ccagaagatc
     9301 gtcccggggc aggccgttcc ggacaatccg atcatcccct tcatcgaggg cgacggcatc
     9361 ggcgtcgaca tcaccccggt gatgatcaag gtgatcgacg cggcggtgga caaggcctac
     9421 ggcggcaaga agaaaatcca ctggatggag gtctacgccg gcgagaagtc gacgcagatc
     9481 tacggtcccg acgagtgg
//
LOCUS       DAOQNN010000085         9405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3551_length_9405_cov_9.740428, whole genome shotgun sequence.
ACCESSION   DAOQNN010000085 DAOQNN010000000
VERSION     DAOQNN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3551_length_9405_cov_9.740428"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1528)
                     /locus_tag="PKV97_06635"
     CDS             complement(119..1528)
                     /locus_tag="PKV97_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNB05590.1"
                     /translation="MSDELNARLRAIVGAANVLDGETDMAPFLSDWRGRYHGRARAVV
                     RPRDTAEVAAVVAACAQAGVAMVPQGGNTGLCGGATPLADGAAVVINLARLDRVRALD
                     PDNDTLTVEAGCTLAAVQEAAQAAGRLFPLSLASEGSCLIGGNLSTNAGGVQVLRYGN
                     TRDLVLGLEVVLPDGRVWDGLRALRKDNTGYDLKQLFIGAEGTLGIVTAAVLKLFPAI
                     RTRATAWVAVADPRAAVRLLGLLRAACGDRVSAFEIVGRTALGLVLRHIPGARDPLAG
                     APAWTVLVELSDPAVDAPLEAQLEAVLGEAVAQGLASDAVVAASVAQARALWALREDI
                     SEAQRIEGVSIKHDVSVPVSRIPEFLERAGAALAARWPDIRVVAFGHIGDGNLHYNLS
                     KAVADANATFIARTAEVNGIVHDLVCELGGSISAEHGLGQLKREEVLRYKPALEMELM
                     RRVKQAFDPAGLMNPGKVL"
     gene            complement(1536..2510)
                     /gene="rssA"
                     /locus_tag="PKV97_06640"
     CDS             complement(1536..2510)
                     /gene="rssA"
                     /locus_tag="PKV97_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HNB05591.1"
                     /translation="MLSGAGSVGLACAEPSGGGPVIGLALGSGAARGWAHLGVLLELA
                     REGVVPQIITGCSIGAFVGAAAASGDLDKLVRWAETLKWQDVVSLLDVSLRGGLIKGQ
                     KLIDFFERNFVDRDFTELDQRFACVATELSTGREIWLHEGSVSAAVRASIALPGLMTP
                     VLHQGRMLVDGGLVNPVPVSLCRAMGADVVIAVDLGSDMVGRAFRRSAVEPAPVEETE
                     AGWTERLLARFGFAGEAEPGMPSRPLAAADSLPSLVSVISSSINIMQVRIARSRLAGE
                     PADLLVSPRVSQLGLMDYHRADEAIAEGVAAVARVRPLLRELLGRANE"
     gene            complement(2541..3872)
                     /gene="rimO"
                     /locus_tag="PKV97_06645"
     CDS             complement(2541..3872)
                     /gene="rimO"
                     /locus_tag="PKV97_06645"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNB05592.1"
                     /translation="MTTFKTSDLPRVGFVSLGCPKATVDSEHILTRLRAEGYAISGSY
                     DDADLVVVNTCGFIDAAVEESLDAIGEALAENGKVIVTGCLGAKDDVILAAHPQVLAV
                     TGPHATEEVMHAVHRHLPKPHDPFTDLVPPQGIRLTPQHYAYLKISEGCNHRCSFCII
                     PSMRGDLVSRPIHEVMREAEALADAGVKELLVISQDTSAYGVDVKYRTGFWGGRPVKT
                     RLLDLAKALGELGIWIRLHYVYPYPSVDDLIPLMAEGKILPYLDVPFQHASPRILKAM
                     KRPANAENVLERVRKWRSICPELTIRSTFITGFPGETEEDFEMLLNFLEEAQLDRVGA
                     FAYSPVEGATANELPGAVPDEVREERRARLMDFQEDISTQRLEAKIDREMTVLVDEVD
                     EEGALARSPGDAPEIDGLVVIPEGDGLAPGDFVRVRITDCDVHDLYAERIE"
     gene            complement(3924..4517)
                     /gene="phaR"
                     /locus_tag="PKV97_06650"
     CDS             complement(3924..4517)
                     /gene="phaR"
                     /locus_tag="PKV97_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HNB05593.1"
                     /translation="MPEQQRLIKKYPNRRLYDTRTSSYITLADVKDLVLGHEHFQVVD
                     AKTGEDLTRSILLQIILEEEAGGAPMFTSDLLAHMIRFYGNAMQGMMGKYLESNIKAF
                     TEMQVKLQDQARAIYGENSPVGQDLWAQFLNFQGPALQSMMGAYVDQSKKMFEQMQTQ
                     LESQTRNMFTGFQFPGYVRPEDAESAAPAALKGDGKK"
     gene            complement(4648..5388)
                     /locus_tag="PKV97_06655"
     CDS             complement(4648..5388)
                     /locus_tag="PKV97_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HNB05594.1"
                     /translation="MSKKIALVTGAMGGLGTAICQSLAKDGFKVVANCLPNFELKDAW
                     LSAQRDLGFDFAAAEGDVSNYESCAAMVAKIEADVGPIDVLVNNAGITRDKFFPKMEK
                     GQWDAVINTNLNSLFNVTHHVSAKMAERGWGRIINISSVNGVKGQAGQTNYSTAKAGV
                     LGFTKALAAELATKGVTVNAIAPGYIGTDMVMAIREDIRQGIIDTVPMKRLGRPDEIG
                     DLCAYLASDKAAYITGATININGGLHMC"
     gene            complement(5627..6367)
                     /gene="phbB"
                     /locus_tag="PKV97_06660"
     CDS             complement(5627..6367)
                     /gene="phbB"
                     /locus_tag="PKV97_06660"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HNB05595.1"
                     /translation="MARVALVTGGMGGLGEAICIKLAALGYKVVTTHSPGNTKAQEWL
                     QTMNNMGYGFKAVPCDVADFDSCKACVEQVVKEVGPVDVLVNNAGITRDMTFKKMTKA
                     DWDAVISTNLDSAFNMTKQVMDGMVERKWGRVINVSSVNGQKGAFGQTNYAAAKAGMH
                     GFTKALALEVARNGVTVNTISPGYIGTKMVMAIPQEILDSKILPQIPVSRLGKPEEIA
                     GLVAYLSSDEAAFVTGANISINGGQHMF"
     gene            complement(6483..8228)
                     /gene="phaC"
                     /locus_tag="PKV97_06665"
     CDS             complement(6483..8228)
                     /gene="phaC"
                     /locus_tag="PKV97_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNB05596.1"
                     /translation="MSDTTGKQVPPGMQAILAAGQAMAQGFFDALARQHVAMQDSSGV
                     AAQKLPMPETEVMASLQKVFAEKHAELWSSMLARTPGAAGPAVVQPEPGDKRFAAPEW
                     SESPVFDYMRQAYLLNAGFLRQMADAMPIADGRAKARMQFLTRQYIDALSPSNFAATN
                     PEFIKTAVETKGESIARGIQNLLGDLEKGRISMTDDAAFEIGRNLALTPGSVVYENEL
                     MQLIQYAPLTEKVAQTPLLIVPPCINKFYIMDLQPENSLVRFVVEQGFTVFLVSWKNP
                     RPDTGGHYTWDDYLDKGPLAALEVVRSITRVKKPNVLGFCVGGTILTSALAVARARGE
                     EPVASLTLMTTLLDFSDAGELGCLVDEASVTAREAAIGKGGVLKGQELANVFSFLRAN
                     DLVWQYVVGNYLKGNKPVAFDLLYWNSDSTNLPGPFLTWYLRNMYLENNLRVPGKLRM
                     LGHKVDLGKVDVPAYLMAAREDHIVPWKSAYLARNVLGGDTTFVLGASGHIAGAINPA
                     SKNRRSYWTADVRPADPDEWFESAAEHKGSWWLHWAEWLRGHGGKEVAARGRLGSTKY
                     EPIEPAPGRYVKERS"
     gene            8335..8556
                     /locus_tag="PKV97_06670"
     CDS             8335..8556
                     /locus_tag="PKV97_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05597.1"
                     /translation="MYLIPIAWLYVVVLVAAAEDSIVAAVLTFVFWGLAPLALFLWLF
                     GTPARRRRRLQQEREALERNDRAETDADQ"
     gene            complement(8606..>9405)
                     /locus_tag="PKV97_06675"
     CDS             complement(8606..>9405)
                     /locus_tag="PKV97_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB05598.1"
                     /translation="FDLYAERGGNFVDTAINYTNGSSEQLVGEFLGSERARFVVATKY
                     SLNTRPGDPNAGGNHRKNLVQSLETSLKRLRTDYVDLYWLHAWDFMTPVDEVMRGLDD
                     LVRSGKVLYVGISDTPAWVVAQANTMADLRGWSAFIGLQIEYSLLERTVERMGVTPRR
                     VRAWLGPAIGPTAFEVGDEVRAAFVGADPGATAAFVAGGVPGKWLADLCALARRRLTL
                     AGVERIDGGSVCTVADPAHFYSYRRDGTTGRFAALIWLEEGRPGAIA"
BASE COUNT         1483 a         3250 c         3113 g         1559 t
ORIGIN      
        1 aaccgctgac ctcttgcatg ccatgcaagc gctctcccag ctgagctata cccccggcac
       61 caaagagcgc gcattatagg tcgctccccg agacctgtaa accccctctc cgaactttct
      121 acagcacctt gcccggattc atcaaaccgg ccggatcgaa ggcctgcttc acccgccgca
      181 tcagctccat ctcgagcgcc ggcttgtagc gcagcacctc ctcgcgcttg agctggccga
      241 gaccgtgctc ggcagagatc gagccgccga gttcgcaaac caggtcgtgc acgatgccat
      301 tgacctctgc ggtgcgtgca atgaaggtgg cgttggcgtc ggccaccgcc ttggacaggt
      361 tgtagtgcag gttgccgtcg ccgatgtgcc cgaaggccac cacgcgaatg tccggccagc
      421 gcgcggcaag cgccgcgccg gcgcgttcga ggaactcggg gatgcggctg accggcaccg
      481 agacgtcgtg cttgatgctg acgccctcga tgcgctgcgc ctcggagatg tcctcgcgca
      541 gcgcccacaa ggcccgcgcc tgcgccacgc tcgccgccac cacggcgtcg ctggccaggc
      601 cctgcgcgac ggcctcgccc agcaccgcct cgagttgggc ctcgagcggc gcatcgacgg
      661 ccggatcgga gagctcgacc agcaccgtcc acgccggcgc accggcaagc ggatcacgcg
      721 cgcccgggat gtgccggagc acgagcccga gcgccgtgcg gccgacaatc tcgaacgcgc
      781 tcacgcggtc gccgcaggct gcccgcaaga gcccgagcag gcgcaccgcc gcgcgcgggt
      841 cggccaccgc cacccaggcc gtggcgcgag tgcggatggc cgggaagagc ttcagcaccg
      901 cggcggtgac gatgcccagg gtgccctcgg cgccgatgaa gagctgcttg aggtcgtagc
      961 cggtgttgtc cttgcgcaga gcacgcagcc cgtcccagac ccggccgtcg ggcagcacca
     1021 cctcaagccc gagcaccagg tcgcgcgtgt tgccgtagcg cagcacctgc acgccgccgg
     1081 cgttggtcga caggttgcca ccgatcaggc aactgccctc cgaggcgagc gagagcggga
     1141 acaggcgtcc ggcggcctgc gccgcctcct gtaccgcggc gagcgtgcaa ccggcctcga
     1201 cggtgagcgt gtcgttgtcg gggtcgagcg cacgaacacg gtccaggcgc gcgagattga
     1261 tcaccaccgc cgcgccatcg gcgagcgggg tcgcgccgcc gcacaggccg gtgttgccgc
     1321 cctgcggcac catcgccacg cccgcctgcg cgcaggcggc gaccaccgcg gccacctcgg
     1381 cggtatcgcg cggacgcacc accgcgcgcg cgcggccgtg gtagcgcccg cgccagtcgc
     1441 tcaggaaggg cgccatgtcg gtttcgccgt cgagcacgtt ggccgcgccg acgatcgcgc
     1501 gcaggcgcgc attcagttcg tcgctcatcc gatcctcact cgttggcgcg gccgagcagc
     1561 tcgcgcaaca acgggcgcac ccgcgccacc gccgcgacgc cctcggcgat cgcctcgtcg
     1621 gcgcggtgat agtccatcaa gccgagctgg ctcacccgcg gcgacaccag caggtccgcg
     1681 ggctcgccgg cgaggcggct gcgcgcgatg cgcacctgca tgatgttgat gctggagctg
     1741 atcaccgaga ccagcgaggg aaggctgtcg gccgcagcca ggggccgcga gggcatgccg
     1801 ggctcggctt cgccggcgaa gccgaagcgc gcgagcaggc gctcggtcca ccccgcctcg
     1861 gtctcctcga ccggcgcagg ctcgaccgcc gagcgccgga aggcgcgccc caccatgtcc
     1921 gagccgagat ccaccgcgat cacgacgtcg gcccccatcg cgcggcacag cgaaaccggc
     1981 accggattga ccaggccgcc atcgaccaac atgcggccct ggtgcagcac cggcgtcatc
     2041 agcccgggga gcgcgatcga cgcacgcacc gcggccgaca cgctgccctc gtgcagccag
     2101 atctcgcgtc cggtggagag ttcagtggca acgcaggcga agcgctggtc gagctcggtg
     2161 aagtcgcggt cgacgaagtt gcgctcgaag aagtcgatca gcttctggcc cttgatgagc
     2221 cccccgcgca gcgacacgtc gagcagcgac accacgtcct gccacttgag ggtctcggcc
     2281 cagcgcacca gcttgtcgag gtcacccgag gccgcggcgg cgcccacgaa ggcaccgatc
     2341 gagcagccgg tgatgatctg cggcaccacg ccttcacgcg caagttcgag caacacgccc
     2401 aagtgcgccc agccgcgcgc ggcaccgctg ccgagcgcca ggccgatcac cggcccgcca
     2461 cccgagggct cggcgcaggc cagcccgaca gagcccgccc cactcagcac ggcgagggct
     2521 ccggcacgga aatgccggcc ttactcgatg cgctcggcgt agaggtcgtg cacatcgcag
     2581 tcggtgatgc gcacccgcac gaagtcgccc ggcgcgaggc cgtcgccctc ggggatgacg
     2641 accaggccgt cgatctcggg cgcatcgccc ggcgagcgcg cgagcgcgcc ctcctcgtcg
     2701 acctcgtcga ccagcacggt catctcgcgg tcgatcttgg cctcgaggcg ctgggtggag
     2761 atgtcctcct ggaagtccat caggcgcgcg cggcgttcct cgcgcacctc gtccggcacc
     2821 gcgccgggca gctcgttggc ggtcgcgccc tcgaccggcg aataggcgaa ggcgccgacg
     2881 cggtcgagct gggcctcttc gaggaagttc agcagcatct cgaagtcttc ctccgtctcg
     2941 ccggggaagc cggtgatgaa ggtcgagcgg atggtgagct cggggcagat gctgcgccac
     3001 ttgcgcacgc gctcgagcac gttctcggca ttggccgggc gcttcatggc cttgaggatg
     3061 cgcgggctgg cgtgctggaa gggcacgtcg aggtagggca ggatcttgcc ttcggccatc
     3121 agcgggatga gatcatccac gctcggatac ggatacacgt aatgcagccg gatccagatg
     3181 ccgagctcgc cgagcgcctt cgccaggtcg agcagccggg tcttcaccgg gcgcccgccc
     3241 cagaagccgg tgcggtactt gacgtccacg ccataggccg aggtgtcctg cgagatcacc
     3301 agcagttcct tgacgcccgc gtcggcgagc gcctcggcct cgcgcatgac ctcgtggatc
     3361 ggacggctga cgaggtcgcc gcgcatcgac gggatgatgc agaagctgca gcggtggttg
     3421 cagccctcgg agatcttgag ataggcgtaa tgctgcggtg tcaggcggat gccttgcggc
     3481 ggcacgaggt cggtgaacgg gtcgtgcggc ttgggcaggt ggcgatgcac cgcgtgcatg
     3541 acctcctcgg tggcgtgcgg gccggtgacc gcgagcacct gcggatgcgc ggcgaggatc
     3601 acatcgtcct tggcgccgag gcagccggtg acgatgacct tgccgttctc ggcgagcgcc
     3661 tcgccgatcg cgtcgagcga ttcctcgacc gcggcatcga tgaagccgca ggtattgacg
     3721 acgaccaggt cggcgtcgtc gtaactgccc gagatcgcat agccctctgc acgcagccgg
     3781 gtaaggatgt gctcggagtc gaccgtcgcc ttggggcagc cgagggaaac gaagccgacg
     3841 cgcggcaggt cggaagtctt gaaggtggtc atgcaaagaa cctgtcgggt cggggcggcg
     3901 gcctcagcgg cgcgcggcgc cgctcacttc ttgccgtcgc ccttgagcgc ggcgggcgcg
     3961 gcggattcgg cgtcctcggg acggacgtag cccgggaact ggaagccggt gaacatgttg
     4021 cgcgtctggc tctcgagttg cgtctgcatc tgctcgaaca tcttcttcga ctggtcgacg
     4081 taggcgccca tcatgctctg caacgcgggc ccctggaagt tgaggaactg cgcccacagg
     4141 tcctggccca ccgggctgtt ctcgccatag atcgcgcgcg cctggtcctg gagcttgacc
     4201 tgcatctcgg tgaaggcctt gatgttgctc tcgaggtact tgcccatcat gccctgcatg
     4261 gcattgccgt agaagcggat catgtgcgcg agcaggtcgc tggtgaacat cggcgcgccc
     4321 cccgcctctt cctcgaggat gatctggagc aggatgctgc gcgtcaggtc ctcgccggtc
     4381 ttcgcatcga ccacctggaa gtgctcgtga ccgagcacca agtccttgac gtcggccagg
     4441 gtgatgtagg aactggtccg cgtgtcgtac agacggcggt tcgggtactt cttgatcagt
     4501 cgctgctgtt ccggcatttc tccgtctcct cggcggcggt gtcttcgctc tgtgcgaaaa
     4561 aattatatcg cgctgcgaca tcgaaaccac gaaaaaaccc cgtctttcga cggggttgcg
     4621 tgatttcgcg catgacacgg cgcaacatca gcacatgtgc aggccaccgt tgatgttgat
     4681 ggtcgccccg gtgatgtagg cagccttgtc ggaggcgagg taggcgcaca ggtcgccgat
     4741 ctcgtccgga cggccgaggc gcttcatcgg cacggtgtcg atgatgccct ggcggatgtc
     4801 ttcgcggatc gccatcacca tgtcggtacc gatgtagccc ggggcgatcg cgttgaccgt
     4861 cacacccttg gtcgccagct cggcggcgag cgccttggtg aagcccagca cgccggcctt
     4921 ggcggtcgag tagttggtct ggcccgcctg acccttgacg ccattgaccg aagagatgtt
     4981 gatgatgcga ccccagccgc gctcggccat cttcgcggag acgtggtggg tgacgttgaa
     5041 caggctgttc agattggtgt tgatcaccgc atcccactgg cccttctcca tcttggggaa
     5101 gaacttgtcg cgggtgatgc cggcgttgtt caccagcaca tcgatcggac cgacgtcggc
     5161 ttcgatcttg gcgaccatcg ccgcgcacga ctcgtagttc gacacgtcgc cttcggcggc
     5221 ggcgaagtcg aagccgaggt cgcgctgagc ggacagccag gcgtccttta gctcgaagtt
     5281 cggcaggcag ttggccacga ccttgaagcc gtccttggca agcgactggc agatcgccgt
     5341 accgagacca cccatggcgc cggtgacaag agcgattttc ttggacatct tgaaatcctt
     5401 caggagcatt tgtagaaacc gaggccgcct gcgggccggg atagccggct tgcgcttcgg
     5461 tcgcggatgc ggcagcgcat cagccacctc acgcgcggat tatagcccct gtttcacaac
     5521 tcatgttgcg atgcaatagc cccattcccg cgccccgcga gcacaaaaaa aagcgcgctc
     5581 caccggagcg cgccacgttg cggagccgga accggccgcc gacgggtcag aacatgtgct
     5641 ggccgccgtt gatagagatg ttggcaccgg tcacgaaggc cgcctcgtcg gaggacaggt
     5701 aggccaccag gccggcgatc tcttccggct tgcccaggcg agacaccggg atctgcggca
     5761 ggatcttgga gtcgaggatc tcctgcggga tcgccatcac catcttggtg ccgatgtagc
     5821 ccggcgagat ggtgttgacg gtgacgccgt tacgcgccac ttccagcgcc agcgccttgg
     5881 tgaaaccgtg catgccggcc ttggccgcgg cgtagttggt ctggccgaag gcgcccttct
     5941 ggccgttgac cgaggacacg ttgatcacgc ggccccactt gcgctcgacc atgccgtcca
     6001 tgacctgctt ggtcatgttg aaggcggagt cgaggttggt gctgatgacc gcatcccagt
     6061 cggccttggt catcttcttg aaggtcatgt cgcgggtgat gccggcgttg ttgaccagca
     6121 cgtcgaccgg accgacctcc ttgaccactt gctcgacgca ggccttgcac gagtcgaaat
     6181 ccgccacgtc gcagggaacg gccttgaagc cgtagcccat gttgttcatg gtctgcagcc
     6241 actcctgggc cttggtgttg cccggggaat gggtcgtcac caccttgtag ccgagcgcgg
     6301 ccagcttgat gcagatcgcc tcgccgagac cgcccatgcc gccggttacc agtgcaactc
     6361 ttgccatctt cttctcctct ccctatgcgg ggtactttcg tagtgctgga cggaccttga
     6421 acacgacggc agcgaccgcc cgatccgctg ccgtcgaaca ctgaaaacct cctcgttccg
     6481 ggtcaggaac gctccttgac gtagcgcccc ggcgccggct cgatcggctc gtacttcgtg
     6541 ctgcccaggc gtccgcgcgc ggccacctcc ttgccgccat ggccgcgcag ccactcggcc
     6601 cagtgcagcc accagctgcc cttgtgctcg gccgcgctct cgaaccattc gtcggggtcg
     6661 gccgggcgca catcggcggt ccagtagctg cggcgattct tcgaagccgg gttgatcgcg
     6721 ccggcgatgt ggccgcttgc gccgagcacg aaggtggtgt cgccgcccag cacgttgcgc
     6781 gccaggtagg cgctcttcca cggcacgatg tggtcctcgc gcgccgccat caggtaggcc
     6841 ggcacgtcga ccttgccgag gtcgaccttg tgcccgagca tcctgagctt gcccggcacg
     6901 cgcaggttgt tctcgaggta catgttgcgc aggtaccagg tgaggaaggg ccccggcagg
     6961 ttggtcgaat cggagttcca gtacagcagg tcgaaggcga ccggcttgtt gcccttgagg
     7021 tagttgccga ccacgtattg ccacaccagg tcgttggcgc gcaggaagga aaacacgttg
     7081 gcgagttcct gccccttgag caccccgccc ttgccgatcg cggcctcgcg cgcggtgacg
     7141 ctcgcctcgt cgaccaggca gccgagctcg ccggcgtcgg agaaatccag cagcgtggtc
     7201 atcaaggtga ggctcgccac cggctcctcg ccgcgcgcgc gcgccaccgc gagcgccgag
     7261 gtgaggatgg tgccgccgac acagaagccg agcacgttgg gcttcttgac gcgggtgatc
     7321 gagcgcacca cctcgagcgc cgcgagcggt cccttgtcga ggtagtcgtc ccaggtgtaa
     7381 tggccgccgg tgtcgggacg ggggttcttc cacgacacca ggaagaccgt gaagccctgc
     7441 tcgacgacga agcgcacgag cgagttctcg ggctgcaggt ccatgatgta gaacttgttg
     7501 atgcacggcg gcacgatcag cagcggcgtc tgcgcgacct tctcggtcag cggcgcgtac
     7561 tggatcagct gcatcagctc gttctcgtag accaccgaac ccggggtcag cgcgaggtta
     7621 cggccgatct cgaaggccgc gtcgtcggtc atcgagatgc gccccttctc gaggtcgccg
     7681 agcaggttct ggatgccgcg cgcgatgctc tcgcccttgg tctcgaccgc ggtcttgatg
     7741 aactcggggt tggtcgccgc gaagttgctc ggcgacagcg catcgatgta ctggcgggtg
     7801 aggaactgca tgcgcgcctt ggcgcggccg tcggcgatcg gcatcgcgtc cgccatctgg
     7861 cgcaggaagc cggcgttgag caggtaggct tggcgcatgt agtcgaacac cgggctctcg
     7921 gaccactccg gcgccgcgaa gcgcttgtcg cccggctcgg gctgcaccac cgcggggcct
     7981 gccgcccccg gcgtgcgcgc aagcatggac gaccacagtt cggcgtgctt ctcggcgaag
     8041 accttctgca gcgaggccat cacctcggtc tcgggcatcg gcagcttctg cgccgccacc
     8101 cccgaggaat cctgcatcgc cacatgctgg cgtgcgagcg catcgaagaa accctgcgcc
     8161 atcgcctgtc ccgcggcgag gatcgcctgc attcccggcg gcacctgctt gcccgtcgta
     8221 tccgacatcc ctttctcctg aggccgcgtc cgcccgctcc gggccggggc cgcggtttcc
     8281 tgccctgtcc gtgatcctcc ggcgccggct cgcggcgcgc gctagaatcc acccatgtat
     8341 ctgatcccga tcgcctggct ctacgtcgtc gtcctcgtcg ccgcggcgga ggattcgatc
     8401 gttgccgccg tcctgacctt cgtgttctgg gggctcgcgc ccctggccct gtttctttgg
     8461 ctgttcggca ccccggcccg ccgacgtcgc cgcctgcagc aggagcgaga agcactcgaa
     8521 cgcaacgata gagccgaaac tgacgccgat caatagccgc aatccctaag acggtcatgc
     8581 gagacggatc gcggcaacgc cgcgctcacg cgatcgcgcc gggccgcccc tcctccagcc
     8641 agatcagcgc ggcgaaacgc ccggtcgtcc catcacgccg gtaagaatag aaatgcgccg
     8701 gatcggccac ggtgcacacg ctgccgccgt cgatccgctc cacgcccgca agtgtgagcc
     8761 gtcgacgcgc gagcgcgcag agatcggcca gccacttccc cggcacgcca cccgcgacga
     8821 aggcggcggt cgcgcccggg tccgcgccga cgaaggccgc gcgcacctcg tccccgacct
     8881 cgaaggccgt cggcccgatc gcgggcccga gccacgcgcg cacgcgcctc ggcgtcacgc
     8941 ccatgcgctc cacggtgcgc tccagcaggc tgtactcaat ctgcaggcca atgaacgcgc
     9001 tccagccccg caaatccgcc atggtgttgg cctgcgccac cacccaggcc ggcgtatcgc
     9061 tgatgcccac gtagaggacc ttgccgctgc gcaccaggtc atccagcccg cgcatcacct
     9121 catccaccgg cgtcatgaag tcccacgcat gcagccaata gaggtccacg tagtccgtgc
     9181 gcaggcgctt gagcgaggtc tccagagact gcaccaggtt cttgcggtgg ttgccgcccg
     9241 cgttggggtc tccggggcgc gtgttgagcg agtacttggt ggccaccacg aagcgcgctc
     9301 gctcggaccc gaggaactct cccacgagct gctcggacga gccgttggtg tagttgatcg
     9361 ccgtgtcgac gaagttgcct ccgcgctcgg cgtagaggtc gaact
//
LOCUS       DAOQNN010000086         9401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3553_length_9401_cov_9.823561, whole genome shotgun sequence.
ACCESSION   DAOQNN010000086 DAOQNN010000000
VERSION     DAOQNN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3553_length_9401_cov_9.823561"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PKV97_06680"
     CDS             complement(<1..744)
                     /locus_tag="PKV97_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB05599.1"
                     /translation="MNESECAGRLIWFKATAGNERFHTYVFQQRVGVLIDWFRVLRSD
                     ERGDRFRAWGIINDPACCVPGSPDCPAKSLDETYGLDWCPGDEELLPHVGRSGYRDPA
                     CDFRDAALKPGDVHGPADQRHDACDLRFGTSTGALGIRKFPNPRFDRAAWLKLNGSPG
                     TWEGYNRPIAAAGGRGEAARSHLQDGAIEPPFLIGTSCGSCHIAFDPLNPPADPARPA
                     WENIKGLIGNQYTRVSEIMVSGMASNTLEW"
     gene            complement(1230..3047)
                     /gene="typA"
                     /locus_tag="PKV97_06685"
     CDS             complement(1230..3047)
                     /gene="typA"
                     /locus_tag="PKV97_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNB05600.1"
                     /translation="MSRAIRNIAIIAHVDHGKTTLVDQLLRQSGTFRENQQVAERVMD
                     SGDIEKERGITILSKNCAIQYGDTHINIVDTPGHADFGGEVERVLSMVDSVLLLVDAV
                     EGPMPQTRFVTRKALALGLKPIVVINKIDRPGARPDWVINHTFDLFDKLGATEEQLDF
                     PVIYASGLNGFAVINEGDERKDMRPLFEAILEHVPAPEVDANGPLQIQVCSLDYSTYM
                     GQLGIGRIKRGSVRPNQEVVVMYGEENRGKGKVSQILTFEGLERKPSELAEAGDIVLI
                     AGIQQVNIGVTLCDPECLEGMKPIAVDEPTLTMNFMVNTSPLAGREGKYVTSRQIRER
                     LEKELLKNVALRVNYTGDSDVFEVSGRGELHLTILLENMRREGYELAVGRPRVVYKEI
                     DGVKCEPYEMLTVDVEEDHQGSVMEELGRRRGELQDMQPDGRGRVRLEYRIPARGLIG
                     FQGEFLTMTRGTGLASHVFDDYGPMAGAMAERRNGVLISQNDGDAVAYALWNLQDRGR
                     MFVSPGEALYEGMIIGIHTRDNDLVVNPIKGKQLTNVRASGTDEAVRLITPIQLTLES
                     AIEFIADDELVEITPKNIRLRKRHLKETDRKRAAKADAS"
     gene            3169..3906
                     /locus_tag="PKV97_06690"
     CDS             3169..3906
                     /locus_tag="PKV97_06690"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HNB05601.1"
                     /translation="MLAIIGGSGLTQLSNMEIERREVVRTPYGEPSGALAFGRVCDEP
                     VVFLARHGYGHTIPPHLVNYRANIWALKQAKATGIVSVASVGGIRADLAPGTLIVPNQ
                     IIDYTWGRKSTFFEGGDTPVRHIDFTEPYDETLRHRLLAAIAAAGDSAMDGGVYASTQ
                     GPRLETAAEIDRLERDGCDIVGMTGMPEAALARELELPYAAIGVVVNYAAGRSSSAHG
                     IHFDDIELVLQESMVRVRAVLEGLCHV"
     gene            3932..4438
                     /locus_tag="PKV97_06695"
     CDS             3932..4438
                     /locus_tag="PKV97_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNB05602.1"
                     /translation="MKRIESGFEHVLFTSRWLMAPVYFGLVLAMVLLLVKFSKELFGL
                     FAQIWSASGSELIIGVLSLVDVALIMNLLIIIMFSGYENFVSKMEDLHTHRDRPEWMG
                     HIGFSDLKLKLIGSIVAISGIELLKAFMNAHNLTDRQLGWMVGIHVTFLFSGLLYAAM
                     DRLAGKGH"
     gene            complement(4478..7054)
                     /locus_tag="PKV97_06700"
     CDS             complement(4478..7054)
                     /locus_tag="PKV97_06700"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNB05603.1"
                     /translation="MNDALTDELHTLATTARQRLVEGTAQLRDAYQGRPQTSILLKGR
                     ARLVDHELVRLWTASAMPASAALVAVGGYGRGELFPASDVDLLVLLSTPPDEALKARL
                     SALVGALWDCGLEVGHAVRTVDECMIAAADDITVQTNLLEARLLAGDPALFAELSARY
                     REALDVRAFYKAKLLEQEQRYARFNDTPYALEPNCKESPGGLRDLQMLGWIARAAGLG
                     HSWRDLARHRLITGSEAGELRAIERFLQHIRIRLHHLTGRAEDRLLFDHQEKLARILG
                     IEATPTRRASEVLMQRYYVTAKMVMQLNTVLLQNYGSVIFPDRGAAIVVNARFQVVRE
                     LLDIRDEDVFERHPSALLECFLLLQQRSELKGMTARTLRALWIHRNQVNAAFRADPAH
                     RVLFLQILQQRRGIVRVFRKMNEYGILSRYIPAWRRIVGQMQHDLFHVYTVDQHILMV
                     LRNVRRFTMGEHAHEYPLMTRLIMAFERHWLLYVAALFHDIAKGRGGDHSTLGMADAR
                     EFCTQHGMDANDTALVVWLVEQHLTMSRVAQKEDTSDPEVIQRFADVVRDERRLVALY
                     LLTHADIRGTSPKVWNGWKGKLLEDLFFATQRLLRGATAKEALGLDGRLEDARRQLRF
                     HGLRPGVEDALWARLDPVYFMRHSAEEIAWHTRLLYYRTESDEPVVKARVAEDEDGVQ
                     VMVFTRDQKDLFVRLTGFFGRMGFTIVDAKVHTTRHGYALDSFMLQDPGEQAAYRDIV
                     ILIEHELTARLQKPGLPERPSRGRMSRQVKHFPISPEVSIRPDEAGRHHVLSLTAADR
                     PGLLYDVAAVLAEHGIRLHTAKIATLGERVEDTFLLTGNVLNQDAQVLRIERELLERL
                     QV"
     gene            complement(7077..7907)
                     /gene="map"
                     /locus_tag="PKV97_06705"
     CDS             complement(7077..7907)
                     /gene="map"
                     /locus_tag="PKV97_06705"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNB05604.1"
                     /translation="MSIVIKTPEEIEKMRVACRLAAEVLDYIEPFVVPGVTTGELDRL
                     CHDYMVNVQGTIPAPLDYAPPGYKPYPKSICTSVNHQVCHGVPGEKALKKGDIVNLDI
                     TVIKEGFHGDTSRMFLLGGDSACSIVARRLTRITYECMWLGIATVKPGGRLGDIGAAI
                     QKHAEGNGFSVVREFCGHGIGRKFHEEPQVLHYGKAGTGPTLEPGMIFTIEPMINAGK
                     AAISELPDGWTIVTKDRSLSAQWEHTVVVTETGVEVLTLSAGCPPPPELVAARFATAG
                     "
     gene            8303..9058
                     /gene="rpsB"
                     /locus_tag="PKV97_06710"
     CDS             8303..9058
                     /gene="rpsB"
                     /locus_tag="PKV97_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNB05605.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRFWNPRMAPYIFGQRNKIHIVNLEK
                     TMAKYNEAMEFVRKLAANRGNIMFVSTKRQAREIIAEEAQRAGMPYVDERWLGGMLTN
                     FKTVKQSIKRLKEVEAMMEDGSIERLSKREALTVRRELEKLQKSIGGIKDMGGLPDAL
                     FVIDVGYHKIAITEAQKLGIPVVAVVDTNHSPEGVDYLIPGNDDSSRAIRLYARGVAD
                     AVLQGRSQVLQEIVDAGGDEFVEVEEEGSQEQA"
     gene            9224..>9401
                     /locus_tag="PKV97_06715"
     CDS             9224..>9401
                     /locus_tag="PKV97_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HNB05606.1"
                     /translation="MAEITASMVKELREKTDAPMMECKKALAEAAGDMGKAEEILRVK
                     LGNKASKAAARIAAE"
BASE COUNT         1493 a         2972 c         3326 g         1610 t
ORIGIN      
        1 ccattcgagg gtgttcgatg ccatgcccga gaccatgatc tcggagacgc gcgtgtattg
       61 gttgccgatc agccccttga tgttctccca tgccgggcgc gccgggtcag ccggggggtt
      121 gagcggatcg aaggcgatgt ggcaggaacc gcaggaggtg ccgatcagga aggggggctc
      181 gatggcaccg tcctggaggt gggagcgggc cgcctcgccc cggccgccgg cggcggcgat
      241 cggccggtta tagccttccc aggtgcccgg gctgccgttg agcttcaacc aagctgcgcg
      301 gtcgaagcgc gggttcggaa acttgcggat gcccagtgcg ccggtcgagg tgccgaaacg
      361 caggtcgcag gcgtcgtggc gctggtcggc tgggccatgc acatcgccgg gcttcagcgc
      421 tgcatcgcgg aagtcgcagg ccggatcgcg gtagccgctg cgcccgacat gggggaggag
      481 ttcctcgtcg cccggacacc agtcgagacc gtaggtctcg tccaggctct tcgccgggca
      541 gtcggggctg ccgggcacgc agcaggccgg atcgttgatg atgccccagg cgcggaagcg
      601 gtcgccgcgc tcgtcgctgc gcagcacgcg gaaccagtcg atcagcacgc ccacccgctg
      661 ctggaacacg taggtgtgga agcgctcgtt gccggcagtg gccttgaacc agatcagccg
      721 tccggcgcat tcggactcgt tcaagccttc ccgcgcgcaa gcctcgtaat atgggaggtc
      781 ctgggcatga attcttgccg gcgtcgacgc gggcgaggcg gcgggcgcat cagcgattgc
      841 caggggaagg ggcggggcga gcatggcggc aatgaagaca acggccgcca acaccacctg
      901 cgatgatgga cgcatcatgt tcgttctcct gctctgtcgg atcggcaggg acgaaggcat
      961 ccgaagcgga cacccgcagg gacgcgctgt gcagatcgtc gggcacaact gcacccgcgc
     1021 tctgcgcttt gcgcagcgaa gctctctgtc ggttctgggc gcgcattacc gcgtgtccgc
     1081 ggcgcgcgct ccattcattt gtggtacttc cgtgatagca ggaaagcggg catttgcaaa
     1141 ccgtgcgctg cgtttgtggc aagcgcccgg atggcaagac gccccgcggg cagaaccggc
     1201 ggggcgtctt tcgttcgagc ggcctgagat caggacgcgt cggccttggc agcgcgcttg
     1261 cggtcggttt ccttcaggtg acgcttgcgc aggcggatgt tcttgggcgt gatttcgacc
     1321 agctcgtcgt cggcgatgaa ctcgatcgca gattccaggg tgagctggat cggcgtgatc
     1381 aggcgcacgg cttcgtccgt acccgaggcg cgcacgttgg tgagctgctt gcccttgatc
     1441 gggttgacca cgaggtcgtt gtcgcgggtg tggatgccga tgatcatgcc ttcgtacagc
     1501 gcctcgcccg ggctgacgaa catgcggcca cggtcctgca ggttccacag cgcgtaggcc
     1561 accgcgtcgc cgtcgttctg cgagatcagc acgccgttgc ggcgctcggc catggcgccg
     1621 gccatcgggc cgtagtcgtc gaagacgtgg ctggcgaggc cggtgccgcg cgtcatggtg
     1681 aggaactcgc cctggaagcc gatcaggccg cgagccggga tgcggtattc gaggcgcacg
     1741 cggcccctgc cgtccggctg catgtcctgg agttcgccac ggcgacggcc gagctcttcc
     1801 atgacgctgc cctggtggtc ttcctcgacg tcgacggtga gcatctcgta cggctcgcac
     1861 ttcacgccgt cgatttcctt gtacaccacg cgcgggcgtc ccaccgccag ctcgtagccc
     1921 tcgcggcgca tgttctcgag caggatggtg aggtggagtt cgccacgacc ggagacctcg
     1981 aacacgtccg agtcgccggt gtaattgacg cgcagggcga cgttcttgag cagttccttt
     2041 tccaggcgct cgcggatctg gcggctggtg acgtacttgc cctcgcgacc ggccagcggc
     2101 gaggtgttca ccatgaagtt catggtcagc gtgggctcgt ccaccgcgat cggcttcatg
     2161 ccttccagac actccgggtc gcacagggtg acgccgatgt tgacctgctg gatgcccgcg
     2221 atcagcacga tgtcgccggc ctcggccagc tcggagggtt tgcgctcgag gccctcgaag
     2281 gtgaggatct ggctgacctt gcccttgccg cggttctctt cgccgtacat gacgacgact
     2341 tcctggttcg ggcgcacgct gccgcgcttg atgcgaccga tgccgagctg gcccatgtag
     2401 gtcgagtagt cgagcgagca gacctggatc tgcagcgggc cgttggcatc gacttccggc
     2461 gcgggcacgt gctcgaggat ggcttcgaac agcgggcgca tgtccttgcg ctcgtctccc
     2521 tcgttgatga ctgcgaagcc gttgaggccg gaggcataga tcaccgggaa gtcgagctgt
     2581 tcctcggtgg cgccgagctt gtcgaagagg tcgaaggtgt ggttgatgac ccagtccggg
     2641 cgtgcgcccg ggcggtcgat cttgttgatg acgacgatcg gcttgagtcc cagcgcgagc
     2701 gccttgcggg tcacgaagcg ggtctgcggc atcgggccct cgacggcgtc gaccagcagc
     2761 agcacgctgt cgaccatcga caggacacgc tcgacctcgc cgccgaagtc ggcgtggccc
     2821 ggggtgtcga cgatgttgat gtgggtatcg ccatactgga tggcgcagtt cttcgacagg
     2881 atcgtgatgc cgcgttcctt ctcgatgtct cccgagtcca tgacgcgttc ggcgacctgc
     2941 tggttttcgc ggaaggtgcc ggactggcgc agcagttgat cgacgagggt ggtcttgccg
     3001 tggtcgacgt gggcgatgat ggcgatgttg cgaatggcgc gggacatgga ggagggcggc
     3061 gaagtctttg aaagggcgag gattctagca cgtgctgtgg cagcgcagca tggttttctg
     3121 aggggcgtgc tagaatcgcg cgcctcagcc aaaggaggtc tcttcaccat gctcgcgatc
     3181 atcggcggca gcggactcac ccaactgtcg aacatggaga tcgagcgccg cgaggtcgtg
     3241 cgcacaccct acggcgagcc ctccggcgca ctcgccttcg ggcgcgtctg cgacgagccg
     3301 gtcgtcttcc tcgcccgcca cggctacggc cacaccatcc cgccgcacct ggtcaattac
     3361 cgcgccaaca tctgggcgct caagcaggcc aaggccaccg gcatcgtctc ggtggcctcg
     3421 gtgggcggca tccgcgccga cctggcgccg ggcacgctga tcgtcccgaa ccagatcatc
     3481 gactacacct gggggcgcaa gagcaccttc ttcgagggcg gcgacacgcc ggtgcgccac
     3541 atcgacttca ccgagcccta cgacgagacc ctgcgccacc gtctgctcgc ggcgatcgct
     3601 gcggccggcg acagcgcgat ggatggcggg gtgtacgcga gcacgcaggg cccgcgcctc
     3661 gagaccgcgg cggagatcga ccgcctcgag cgcgacggct gcgacatcgt cggcatgacc
     3721 ggcatgcccg aggcggcgct ggcgcgcgag ctcgagctgc cctacgcggc gatcggggtg
     3781 gtggtgaact acgccgcggg gcgctcgtcg agcgcgcacg gcatccactt cgacgacatc
     3841 gagctggtgt tgcaggagtc catggtgcgc gtgcgcgccg tgctggaggg gctgtgccat
     3901 gtctgatcga ccggtcgagg agcgcgatgc gatgaagcgg atcgagagcg ggttcgagca
     3961 cgtgctgttc accagccgct ggctgatggc gccggtgtat ttcggcctcg tgctggcgat
     4021 ggtattgctg ctggtgaagt tctccaagga gctgttcggg ctgttcgcgc agatctggag
     4081 cgcctcgggc agcgagctga tcatcggcgt gctgtcgctg gtggacgtgg cgctgatcat
     4141 gaacctgctg atcatcatca tgttcagcgg ctacgagaac ttcgtgtcca agatggagga
     4201 cctgcacacc caccgcgacc gccccgagtg gatgggacac atcggcttct ccgacctcaa
     4261 gctcaagctg atcggctcga tcgtggcgat ctccgggatc gaactgctca aggccttcat
     4321 gaacgcccac aacctcaccg accgccagct cggctggatg gtgggcatcc acgtgacctt
     4381 cctgttctcg ggcctgctct acgccgccat ggatcgcctg gcggggaagg ggcactgagc
     4441 gcgccgcgag cgcatccgga gcgcgccgcg agcgcggcta gacctgcagc cgctccagca
     4501 gctcgcgctc gatcctcagc acctgcgcgt cctggttgag cacgttgccg gtgagcagga
     4561 aggtgtcctc gacgcgctcg cccagggtgg cgatcttggc ggtgtgcagg cggatgccgt
     4621 gctcggcgag caccgcggcg acgtcgtaga gcaggccggg gcgatcggcg gcggtgagcg
     4681 agagcacgtg atggcggccg gcctcgtcgg gacggatgct gacctccggg ctgatcggga
     4741 agtgcttgac ctgcctcgac attctcccgc gactcgggcg ctcgggcagc cccggtttct
     4801 gcaggcgggc ggtgagctcg tgctcgatca ggatcacgat gtcgcggtag gcggcctgtt
     4861 cgcccgggtc ctgcagcatg aagctgtcga gcgcgtagcc gtggcgggtg gtgtgcacct
     4921 tggcgtcgac gatggtgaag cccatgcgcc cgaagaagcc ggtcaggcgc acgaagaggt
     4981 ccttctggtc gcgggtgaac accatgacct gcacgccgtc ctcgtcctcg gccacgcgcg
     5041 ccttcaccac cggctcgtcg gattcggtac ggtagtacaa caagcgggtg tgccaggcga
     5101 tctcctcggc ggagtggcgc atgaagtaca ccgggtcgag ccgggcccac agcgcgtcct
     5161 cgacgccggg gcgcaggccg tggaagcgca actggcggcg ggcgtcctcg aggcggccgt
     5221 cgaggccgag cgcctccttg gcggtggcgc cgcgcagcag gcgctgggtg gcgaagaaca
     5281 gatcctcgag cagcttgccc ttccagccgt tccacacctt ggggctggtg ccgcggatgt
     5341 cggcgtgggt gagcaggtag agcgcgacca ggcgacgctc gtcgcgcacg acgtcggcga
     5401 agcgctggat gacctcgggg tcggaggtgt cttccttctg tgcgacccgc gacatcgtga
     5461 ggtgttgctc gaccagccac acgaccagtg cggtgtcgtt cgcgtccatg ccgtgctggg
     5521 tgcagaattc gcgcgcgtcg gccatgccga gcgtggagtg gtcgccgccg cggcccttgg
     5581 cgatgtcgtg gaagagcgcg gcgacgtaga gcagccagtg gcgctcgaag gccatgatca
     5641 gccgcgtcat cagcgggtat tcgtgcgcgt gctctcccat cgtgaaccgg cgcacgttgc
     5701 gcagcaccat caggatgtgc tggtcgaccg tatagacgtg gaagaggtcg tgctgcatct
     5761 ggccgacgat gcgccgccac gccgggatgt agcgcgacag gatgccgtac tcgttcatct
     5821 tgcggaacac ccgcacgata ccgcggcgct gctgcaggat ctgcaggaag aggacacggt
     5881 gcgcggggtc ggcgcgaaac gcggcattga cctgattgcg gtggatccac agcgcgcgca
     5941 gggtgcgcgc cgtcatgccc ttgagctcgg agcgctgctg cagcagcagg aagcattcga
     6001 gcagcgcgga agggtggcgc tcgaagacgt cctcgtcgcg gatgtcgagc agctcgcgca
     6061 cgacctggaa gcgcgcgttg accacgatcg cggcgccgcg gtcggggaag atcacgctgc
     6121 cgtagttctg cagcagcacg gtgttgagct gcatcaccat cttggcggtg acgtagtagc
     6181 gctgcatcag cacctcggag gcgcgccggg tgggcgtggc ctcgatgccg aggatgcggg
     6241 cgagcttttc ctggtggtcg aagagcaggc ggtcctccgc gcgcccggtg agatgatgca
     6301 ggcggatgcg gatgtgctgc aggaagcgct cgatcgcgcg cagttcgccg gcttcgctgc
     6361 cggtgatgag gcggtggcgg gcgaggtcgc gccagctgtg gccaagcccg gccgcgcgtg
     6421 cgatccagcc cagcatctgc aggtcgcgca gcccgccggg gctctccttg cagttgggct
     6481 cgagggcgta gggcgtgtcg ttgaagcggg cgtagcgttg ttcctgctcg agcagcttgg
     6541 ccttgtagaa ggcgcgcacg tcgagcgctt cgcggtagcg cgcgctcagc tcggcaaaca
     6601 gcgccgggtc gccggcgagc agacgcgcct cgagcaggtt ggtctgcacg gtgatgtcgt
     6661 cggcggcggc gatcatgcat tcgtcgaccg tgcgcaccgc atgaccgacc tcgagcccgc
     6721 agtcccacaa cgcgccgacc agcgccgaca ggcgcgcctt cagcgcctcg tcgggcggtg
     6781 tggatagcag cacgagcagg tcgacgtccg aggcggggaa aagctcgccg cgcccatagc
     6841 cgcccaccgc cacgagcgcg gcggaggcgg gcatcgcgga cgccgtccac aggcggacga
     6901 gctcgtgatc caccagtcga gcgcgaccct tgagcaggat ggaggtctgc ggccgcccct
     6961 gataggcgtc gcgcagctgc gccgtgccct cgacgagacg ttggcgtgcc gtggtggcaa
     7021 gggtgtgcag ctcgtcggtg agggcgtcgt tcatgtgcgg gcctgcgacg gattcttcag
     7081 ccagccgttg cgaaacgggc agcgacgagc tcgggcgggg gcgggcaccc ggcggaaagg
     7141 gtgagcacct cgacaccggt ctcggtgacc accaccgtgt gctcccactg cgccgacagg
     7201 ctgcggtcct tggtgacgat ggtccacccg tcgggcagct cggagatcgc cgccttgccg
     7261 gcgttgatca tcggctcgat ggtgaagatc atgccgggct cgagcgtggg gccggtgccg
     7321 gccttgccgt agtgcagcac ctgcggttcc tcgtggaact tgcgcccgat gccgtggccg
     7381 cagaactcgc gcaccaccga gaagccgttg ccttcggcgt gcttctggat cgcggcgccg
     7441 atgtcgccga ggcggccgcc gggcttgacg gtggcgatgc ccagccacat gcactcgtag
     7501 gtgatccgcg tcaggcggcg ggcgacgatg ctgcacgcag agtcgccgcc gagcaggaac
     7561 atgcggctgg tgtcgccgtg gaagccctcc ttgatcaccg taatgtcgag gttgacgatg
     7621 tcgcccttct tgagcgcctt ctcgcccggt acgccatggc agacctggtg attgaccgaa
     7681 gtgcagatcg acttggggta gggcttgtag ccgggcggtg cgtagtcgag cggggccggg
     7741 atggtgccct gcacgttcac catgtagtcg tggcagaggc ggtcgagttc gccggtggtc
     7801 acgccgggga cgacgaacgg ctcgatgtag tcgagcacct cggcggcgag ccggcaggcc
     7861 acgcgcatct tctcgatctc ttcgggggtc ttgatgacaa tgctcattgt tgtgtgttct
     7921 gtgtcggggc gggaaagcgg gaaggctatc gcaagcgccg cgccgcagca aacccgggat
     7981 ccgcgcaaac cgcatgccaa ccgatgcctt cgccatttcc gcggcgggta cccgcgaggc
     8041 ttgtgtcggc ctgggtttca atccgcttca gccttgagca cgatgcggcc ggcgtgataa
     8101 catcgcgggc ttgtctgcat gtgcaggcaa aatccgcacg ccttgcggca cagggtcctt
     8161 ccggggctcg ctcgacatcc tgcgttccgg ctccggccgg ggcgggggac gcgcaaatcc
     8221 cggggggcag cgtggtgaac cggtttcccg gtcgcccatc cgcgaggcgg aggttcaacc
     8281 cttgtacagt ttggagcaac acatgtccgt cacgatgcgt cagatgctgg aagccggcgt
     8341 ccacttcggt caccagaccc gcttctggaa cccgcgcatg gccccctaca tcttcggcca
     8401 gcgcaacaag atccacatcg tcaacctcga aaagacgatg gcgaagtaca acgaagccat
     8461 ggagttcgtg cgcaagctcg ccgcgaaccg cggcaacatc atgttcgtca gcaccaagcg
     8521 ccaggcgcgc gagatcatcg ccgaggaagc ccagcgcgcc ggcatgccct acgtcgacga
     8581 gcgctggctc ggcggcatgc tgaccaactt caagaccgtc aagcagtcga tcaagcgcct
     8641 caaggaagtc gaggcgatga tggaagacgg ctcgatcgag cgcctgtcca agcgcgaggc
     8701 gctgaccgtg cgccgcgagc tcgagaagct gcagaagtcg atcggcggca tcaaggacat
     8761 gggcggcctg cccgacgcgc tgttcgtcat cgacgtcggc taccacaaga tcgccatcac
     8821 cgaagcccag aagctcggca tcccggtcgt ggccgtggtc gacaccaacc actcgcccga
     8881 aggcgtggat tacctgatcc cgggtaacga cgactcctcg cgcgccatcc gcctctacgc
     8941 ccgtggcgtg gccgatgccg tgctccaggg ccgcagccag gtgctgcagg aaatcgtcga
     9001 tgccggcggc gacgagttcg tcgaggtcga ggaagaaggc tcgcaggaac aggcctgatc
     9061 ctcagcgaag ggggcccgcg gggccccttt tgcaatccgg gtggcgcttg cgaatcccgc
     9121 aacgtgcgcc ccgcacaatt cccgatgtcg cgccgatggc cctccggtgc gctgcgcggc
     9181 atccgcgttt cgaatgtcaa accgaaccta ttcaggagct agcatggcgg aaattaccgc
     9241 aagcatggtc aaggaactgc gcgagaagac cgacgcgccg atgatggaat gcaagaaggc
     9301 gctggccgaa gccgccggcg acatgggcaa ggccgaagag atcctgcgcg tcaagctcgg
     9361 caacaaggcc tccaaggccg ccgcccgcat cgccgccgaa g
//
LOCUS       DAOQNN010000087         9348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3595_length_9348_cov_10.909071, whole genome shotgun sequence.
ACCESSION   DAOQNN010000087 DAOQNN010000000
VERSION     DAOQNN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3595_length_9348_cov_10.909071"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKV97_06720"
     CDS             <1..282
                     /locus_tag="PKV97_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05607.1"
                     /translation="ARLASPPHAMLPAPAAAADNPFERCNRWLLDTALACGGERVWLL
                     CLWDGRRGDGAGGTAHMVEEVSRHRGHVLHIDTRELRPHDPAGSPPLPD"
     gene            complement(436..1065)
                     /locus_tag="PKV97_06725"
     CDS             complement(436..1065)
                     /locus_tag="PKV97_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB05608.1"
                     /translation="MILFVHGLWMHGVVFSAHRRELRRMGRESRVFSWHSLLGSLDGA
                     ADDLAAHIAALGRRPLHLVGHSLGGCVVLNMLARHHPEGIGRVVLLGSPCCGSHAGER
                     ILRVPLLRGALGRVLPQWLARPLPEVPPGYEIGVVAGNRPVGMGRIVPGMEKPNDGMV
                     TVAETRWPGARDHTVLPVTHMQMLWSRACLIQTLHFLDTGRFLRGGVRG"
     gene            1372..2412
                     /locus_tag="PKV97_06730"
     CDS             1372..2412
                     /locus_tag="PKV97_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNB05609.1"
                     /translation="MSNQLATPLRRRILATGLALAAFALAGCSGEQKGTQPATTATNQ
                     KLVIKVGHAATESNTGHKGLVEFNRLLGEKTGGRISLEIYPNSQLGSERELIEAVQLG
                     SVGMTFVSSAPLGGFKKEFFALDLPFVFKDRPTVYKVLDGEPGQYLLKSLQDINIQGL
                     GFWENGFRQLSNSKVAVKTPDDLKGIKMRTMENEVHLAAWKELGANPAPLAFGELFTA
                     LQQGTFDAQETPINLFRDMKFFEVQKFITKTGHLYSPFVILMSKPLYDGLSEADKQAM
                     AEAFEAAKAYQRDLAQKSDAEAEAQMTGITFTELSDAEKEAFRAKMGPVYDLVKKKAG
                     EEIVKKVLQATN"
     gene            2503..2988
                     /locus_tag="PKV97_06735"
     CDS             2503..2988
                     /locus_tag="PKV97_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNB05610.1"
                     /translation="MLKKLDDHFEEALIVLLMAAMTVLIVVQIFMRYVVQESLTWSEE
                     LARYIFVWATYLGVAYGVKKNAHICVEAVTGYLPARIREITYIVAQIVFLLFAALVVK
                     EGFVLSMKIFKFGQSSAALGMPMGVIYLAPTVGFALVFIRLVQNIVAGFKRLRTLGVA
                     A"
     gene            2985..4265
                     /locus_tag="PKV97_06740"
     CDS             2985..4265
                     /locus_tag="PKV97_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNB05611.1"
                     /translation="MISLILFGLFALLMIVGVPIGISLGIAAMAALGYGGVVDSSYLA
                     QSLVTSIDSFPLMAVPFFILAGDLMGHGGISRRLLEVGEILFGRYTGGLGLVAVATCM
                     FFAAISGSGPATVAAIGGILFPAMLKRGYDRNFSVGLVASAGCIGVVIPPSIPMVIYA
                     TSSGASVSSLFLAGVVPGVLIGVVLMLMSWFIARKAGYHGIERSYTGAEIFRILWDAK
                     WALLVPVIILGGIYGGIFTPTEAAAVAVAYGFFVGVFVYKDLKLADVYKVLAHSALTS
                     ATVMVIVGTATVFGRVLAIEGIPVKLANEIVALTSDPIMILLLINVLFLVVGCFMETL
                     AAIIILTPILLPVVLKVGVDPVHFGILMIVNLAIGMVTPPMGINLFVGSRVGNAQLEG
                     VIRGATPFLLSMIVVLLVLTYVPQFTLWLPNLMK"
     gene            4294..5688
                     /locus_tag="PKV97_06745"
     CDS             4294..5688
                     /locus_tag="PKV97_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNB05612.1"
                     /translation="MTALNASSATALEALTAAFGADVLPGDASPKYLQDWSGCAGGQP
                     LAILRPRSTDELSRMLAICHAHGQAVVPQGGLTGLCGGAVPAGGEVVVSLERMNRIER
                     IDIDSGTVIVEAGAILQTVQEACRDAGALLAVDLGARGSCQVGGNVSTNAGGNRVIRY
                     GNTRDLVLGLEVVLADGTVLSMLNQMVKNNAGMDLKHLFIGSEGVLGIVTRVVFKLQP
                     LPKGVSTAVVALHDYPAALRFLRHAQQSLSGQVSAFEIMWNDYLVTAVAAGKLRAPLD
                     TRYPVYVLIDMHCGQPETDAGRFEAMLEQAIEAGWILDGAVAQSVAEAEAFWAIRDGV
                     AEVLRDYAPTLNFDVSVPIAQIGDCAARIRGELDRDWPALKALFFGHVGDGNLHVVVC
                     KVPSDHDSQMRIEDAVYSVVRSYGGSISAEHGIGTLKRDWLHYSRSEAELALMRTLKH
                     TLDPKNILNPGKLI"
     gene            complement(5693..6421)
                     /locus_tag="PKV97_06750"
     CDS             complement(5693..6421)
                     /locus_tag="PKV97_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNB05613.1"
                     /translation="MLRGLDILEAIADGARTVAEIAAQTGLSLSTTHRLASSLADRNY
                     LKFEPRKGYSLGQKLIELGFAGYREADLRKLARPYLEELASRTYDTIHLAALVHDEVT
                     YLDKIAGKRPVEISSRVGGRKPVCSTGVGKALILDRSEDFWRHCYRKEFGGEADPDAM
                     AEWMARMRSYVGLDYAEDFGENEGAIRCVAAPIRDGSQRIVAAVSVSSAREYMDRQRM
                     QGLINEVRNTARAISAELGAPDKD"
     gene            complement(6624..7523)
                     /locus_tag="PKV97_06755"
     CDS             complement(6624..7523)
                     /locus_tag="PKV97_06755"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNB05614.1"
                     /translation="MSKNSPLRARNFPSAEDEAKAAQPQGRYDGPGSSFRMAFTDTEF
                     LLRDELRPVRLQLELLKAELVQQEQGVESTVVVFGSARFKAPDVAEAMLRDALASGDE
                     AATARARQMVKNARWYEEARRFGELVTRESEALGEPVIVATGGGPGIMEAGNRGAFEA
                     GGRSMGMSIFLPFEEAPNPYITPELCFQFHYFAIRKMHFLMRAVALVSFPGGLGTLDE
                     LFEVLTLTQTRKIRRRPIVLIGRDFWQRLIDFDVLVEHGVISPEDKNLFHYAETAEEA
                     WDAIKAAYSGDNPSLTARQLKGN"
     gene            complement(7597..8271)
                     /locus_tag="PKV97_06760"
     CDS             complement(7597..8271)
                     /locus_tag="PKV97_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNB05615.1"
                     /translation="MTTTLYHCINARSFRALWMLEELGLPYALKVLPFPPRSRDEEYL
                     AVNPLGTIPAFLEDDVWMSESAAICQYLAARHSPRALDVGVDEADFGAYLNYLHFGEA
                     TLTFPQTLILRYGRFEPPERRLPQAAEDYTRWFLSRLRTLEPRLETREFLCAGRFTAA
                     DVAVGYALMLATDIGLQDRFKPATLAYWQRLQARPGFLRALEAQTRAALEQGVSTASV
                     ACWDGR"
     gene            complement(8349..>9348)
                     /gene="modC"
                     /locus_tag="PKV97_06765"
     CDS             complement(8349..>9348)
                     /gene="modC"
                     /locus_tag="PKV97_06765"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HNB05616.1"
                     /translation="VTALFGHSGSGKTTLLRCVAGLEPAARGRLVVNGECWQDDARRI
                     RVPTHRRALGYVFQEASLFPHLSVRRNLEFGLHRIPAAARRVPLDHAVALLGIEALLE
                     RMPDRLSGGERQRVGMARALLTSPRILLMDEPLAALDHRRKQEILPYLERLHDELDIP
                     VLYVSHAPDEVVRLADHVVMLGEGKAMAAGELHATMARLDLPTAFTEDAGVVVDGTVA
                     EHDDHYHLTRLDFPGGAVWVQRQPAPPGRRLRFRIHARDVSLADHLVEGSSIQNLLPV
                     IVEEVVGADTPAHVLVRLDAGGTALLARITRRAAEQLGLRPGRRMWAQIKSVALLG"
BASE COUNT         1510 a         3198 c         3162 g         1478 t
ORIGIN      
        1 gcccgcctcg ccagcccgcc gcacgccatg ctgcccgccc ccgcggccgc ggccgacaat
       61 cccttcgagc gctgcaaccg ctggctgctc gacaccgcac tcgcctgcgg tggcgaacgc
      121 gtctggctgc tgtgcctgtg ggacgggcgc cgcggcgacg gcgccggggg caccgcgcat
      181 atggtcgagg aggtaagccg ccaccgcggc cacgtcctcc acatcgacac ccgcgagctg
      241 cgaccgcacg atccggctgg cagccccccg ctgccggact aggctgaatc gagccgggcg
      301 cggcaggccc gcaatccgaa ggtccagcgc agacccctca tgaccgcgac ggcggccacg
      361 cctgggagat cgcctgcggc ggatccgcga cgcgatctga gcccccgccc ccgaccgggg
      421 gcgcgctgcg cgcgatcagc cgcgcacgcc cccgcgcagg aagcgcccgg tgtcgaggaa
      481 gtgcagggtc tggatcaggc aggcgcgcga ccacagcatc tgcatgtggg tgaccggcag
      541 cacggtgtgg tcgcgcgcgc ccggccagcg cgtctcggcc accgtcacca tgccgtcgtt
      601 gggcttctcc atgccgggca cgatgcgccc catgccgacc gggcggttgc cggcgaccac
      661 gccgatctcg tagccgggcg gcacctcggg cagcggccgc gccagccact gcggcagcac
      721 gcggccgagc gcgccgcgca ggagcgggac gcgcaggatg cgctcgcccg catgggaccc
      781 gcagcacggc gaaccgagca ggaccacgcg gccgatgcct tccggatgat ggcgggcgag
      841 catgttcagc acgacacagc cgcccaggct gtgcccgacc agatgcagcg gccggcggcc
      901 gagcgcggcg atgtgcgccg caagatcatc tgccgcgccg tcgagcgagc cgagcagcga
      961 atgccatgag aacacccggc tctcgcgccc catgcggcgc agctcgcgac ggtgcgcgga
     1021 aaacaccacg ccgtgcatcc acaggccgtg gacgaagagg atcacggact ccgcgccggc
     1081 taccacaagc gcccccgcgt tccacgcaca agacaggcag gggcaccctg caggaaaggg
     1141 ggtgtccctc tccttgcgcg cacttcatgc aaatcctgca gctcgacaca ccggataaat
     1201 ctcaatattt gagatttata ttcacatatt gacatggctt tcctcaaacc ccagcattca
     1261 tggctttaat cgattatcca gtttggattc cacccagccg cgtaccggac aaatgcgagt
     1321 cctcgagagc cgcaggcagc ctgtcggtac ccaatgaagg aactacactc catgtccaac
     1381 cagctcgcaa cccccctccg ccgccgcatc ctcgcgacag gcctcgccct cgccgctttc
     1441 gcactcgccg gatgctcggg cgaacagaaa ggcacgcaac ccgcgaccac agcgacgaac
     1501 cagaagctgg tgatcaaggt cggccacgcc gccacggagt ccaacaccgg ccacaagggg
     1561 ctggtcgagt tcaaccgcct gctgggcgaa aagacgggcg gccgcatcag cctggagatc
     1621 taccccaact cgcagctcgg cagcgaacgc gagctgatcg aagcggtgca actgggcagc
     1681 gtcggcatga ccttcgtgtc ctcggccccg ctgggcggct tcaagaagga gttcttcgcc
     1741 ctcgacctgc ccttcgtgtt caaggatcgt cccaccgtct acaaggtact cgacggcgaa
     1801 cccggccagt acctgctgaa gagcctccag gacatcaaca tccagggcct cggcttctgg
     1861 gagaacggtt tccgccagct cagcaacagc aaggtggcgg tgaagacgcc ggatgacctg
     1921 aagggcatca agatgcgcac gatggaaaac gaagtccacc tcgccgcctg gaaggagctg
     1981 ggtgccaacc cggcgccgct ggccttcggc gagctgttca ccgcgctgca gcagggcacc
     2041 ttcgatgccc aggaaacccc gatcaacctg ttccgcgaca tgaagttctt cgaggtgcag
     2101 aagttcatca ccaagaccgg ccacctctac tcgcccttcg tcatcctgat gagcaagccg
     2161 ctctacgacg gtctgagcga ggccgacaag caggccatgg ccgaagcctt cgaagccgcc
     2221 aaggcgtacc agcgcgacct cgcgcagaag agcgacgccg aggccgaagc gcagatgacc
     2281 ggcatcacct tcaccgaatt gagcgacgcc gagaaggaag ccttccgcgc caagatgggg
     2341 ccggtgtacg acctggtgaa gaagaaggcc ggcgaggaaa tcgtcaaaaa ggtgctgcag
     2401 gccaccaact aagcctgcga cgccccggct gcgtgcggcc ggggcgccgt cctttcggcc
     2461 ccggcgttcg gccggcgcgg cccgcgaccc cgagagaccc ccatgctcaa gaaactcgat
     2521 gatcacttcg aggaggcact gatcgtcctg ctgatggcag cgatgaccgt cctcatcgtc
     2581 gtgcagatct tcatgcgcta cgtcgtccag gaatccctga cctggtccga agaactggcc
     2641 cgctatatct tcgtgtgggc gacctacctc ggggtggcct acggcgtgaa gaagaatgcc
     2701 cacatctgcg tggaggcggt gaccggctac ctgcccgccc gcatcaggga gatcacctac
     2761 atcgtggccc agatagtgtt cctgctgttc gctgcgctgg tggtcaagga aggcttcgtg
     2821 ctgagcatga agatcttcaa gttcggccaa tcgtcggccg cgctcggcat gccgatgggg
     2881 gtgatctacc tggcgccgac ggtcggtttc gcgctggtgt tcatccgcct cgttcaaaac
     2941 atcgtcgcgg gattcaagcg cctgcgcacc ttgggggtgg cagcatgatc tccctgatcc
     3001 tgttcggact gttcgccctg ctgatgatcg tgggcgtgcc gatcggcatc tcgctcggca
     3061 tcgcggcgat ggcggcgctc ggctacggcg gcgtggtcga cagcagctat ctggcgcaga
     3121 gcctggtgac ctcgatcgac tccttcccgc tgatggcggt gcccttcttc atcctcgccg
     3181 gcgacctcat gggccacggc ggcatctcgc gccgcctgct cgaagtcggc gagatcctgt
     3241 tcggccgtta caccggcggc ctcggcctgg tcgccgtggc cacctgcatg ttcttcgccg
     3301 cgatctcggg ctcgggcccg gcgacggtgg cggcgatcgg cggcatcctg ttcccggcca
     3361 tgctcaagcg cggctacgac cgcaacttct cggtcggcct ggtcgcgtcc gcaggctgca
     3421 tcggcgtggt gattccgccc agcatcccga tggtgatcta cgccacctcc tccggcgcct
     3481 cggtgtccag cctcttcctc gcgggggtcg tcccgggtgt gctgatcggc gtggtgctga
     3541 tgctgatgag ctggttcatc gcgcgcaagg ccggttacca cggcatcgag cgcagctaca
     3601 ccggcgccga gatcttccgc atcctgtggg acgccaagtg ggcgctgctg gtgccggtga
     3661 tcatcctggg cggcatctac ggcggcatct tcaccccgac cgaagccgcg gcggtagcgg
     3721 tggcctacgg cttcttcgtc ggcgttttcg tctacaagga cctcaagctc gccgatgtct
     3781 acaaagtgct ggcacactcc gccctgacct ccgcgacggt gatggtgatc gtcggcacgg
     3841 cgacggtgtt cggccgcgtg ctggcgatcg aaggcatccc ggtcaagctg gccaacgaga
     3901 tcgtggccct gaccagcgat ccgatcatga tcctgctgct gatcaacgtg ctgttcctgg
     3961 tggtcggctg cttcatggag acgctggcgg cgatcatcat cctgaccccg atcctgctcc
     4021 cggtggtgct gaaggtgggc gtcgatccgg tgcatttcgg catcctgatg atcgtcaacc
     4081 tggcgatcgg gatggtcacc ccgccgatgg gcatcaacct gttcgtcggc tcgcgggtcg
     4141 gcaacgccca actggaaggc gtcattcgtg gcgcgacgcc cttcctgctg tcgatgatcg
     4201 tggtgctgct ggtattgacc tacgtgccgc agttcaccct gtggctgccc aacctgatga
     4261 agtgatcgca acgctgtcga gagagccgag ccgatgaccg cactgaatgc ctcttccgct
     4321 accgcgctgg aagccctgac cgccgctttc ggcgccgacg tgctccccgg cgatgccagt
     4381 ccgaagtacc tgcaggactg gagcggctgc gccggcggcc agccgctggc gatcctgcgc
     4441 ccgcgcagca ccgacgaact gtcgcgcatg ctggcgatct gccacgccca cggccaggcg
     4501 gtggtgccgc agggcggcct caccggcctg tgtggcggcg cggtgccggc cggcggcgag
     4561 gtggtggtgt cgctcgagcg catgaaccgc atcgagcgca tcgacatcga ttcgggcacc
     4621 gtcatcgtcg aggccggggc gatcctgcag acggtgcagg aagcctgccg cgacgccggc
     4681 gcgctgctcg cggtcgacct cggcgcgcgc ggctcctgcc aggtgggcgg caacgtgtcg
     4741 accaacgcgg gcggcaaccg ggtgatccgc tacggcaaca cccgcgacct ggtgctcggg
     4801 ctcgaggtgg tgctcgccga cggcacggtg ttgagcatgc tcaaccagat ggtcaagaac
     4861 aacgccggca tggacctcaa gcatctcttc atcggcagcg agggcgtgct cggcatcgtc
     4921 accagggtgg tgttcaagct gcagccgctg cccaagggcg tgagcaccgc ggtggtcgcg
     4981 ctgcacgact accccgccgc gctgcgcttc ctgcgccatg cacagcagag cctgtcgggc
     5041 caggtcagcg ccttcgagat catgtggaac gactacctgg tcaccgccgt cgccgccggc
     5101 aagctgcgcg caccgctcga cacccgctat ccggtgtacg tgctgatcga catgcactgc
     5161 ggccagccgg agaccgacgc cgggcgcttc gaggcgatgc tggaacaggc catcgaggcg
     5221 ggctggatcc tcgacggcgc ggtggcgcag tcggtggccg aggccgaagc cttctgggcg
     5281 atccgcgatg gcgtcgccga agtgctgcgc gactatgcgc cgacgctcaa cttcgacgtc
     5341 tcggtgccga tcgcgcagat cggcgactgc gcagcgcgca tccgcggcga gctcgaccgc
     5401 gactggccgg cgctcaaggc gctgttcttc ggccacgtcg gcgacggcaa cctgcatgtg
     5461 gtggtgtgca aggtgccgag cgaccacgac agccagatgc gcatcgagga cgcggtgtat
     5521 tcggtggtgc gcagctacgg cggctcgatc tcggccgagc acggcatcgg cacgctcaag
     5581 cgcgactggc tgcactattc gcgcagcgag gccgagctgg cgctgatgcg gaccttgaag
     5641 cacaccctgg atccgaagaa catcctcaat ccaggcaagc tgatctgagc gttcagtcct
     5701 tgtccggtgc gccgagctcc gcgctgatgg cgcgtgcggt gttacgaact tcgttgatca
     5761 gtccctgcat gcgctggcgg tccatgtact cgcgggccga tgaaacgctg accgccgcga
     5821 cgatgcgctg gctgccatcg cggatgggtg cggcgacgca gcggatggcg ccttcgttct
     5881 cgccgaaatc ctcggcgtaa tcgagcccga cgtagctgcg catcctggcc atccattcgg
     5941 ccatggcgtc ggggtcggcc tcgccgccga actccttgcg atagcagtgc cgccagaaat
     6001 cttcactgcg atcgaggatc agggccttgc ccacgccggt ggagcacacc ggcttgcgcc
     6061 caccgacgcg ggaactgatc tccaccgggc gcttgcccgc gatcttgtcg aggtaggtca
     6121 cctcatcgtg gaccagcgcg gcaagatgga tggtgtcgta cgtccggctg gcgagttcct
     6181 ccaggtaagg ccgggccagc ttgcgaagat cggcctcgcg atagcccgca aagcccagct
     6241 cgatgagctt ctggccgagg ctgtagccct tgcgcggctc gaacttgagg tagttgcgat
     6301 cggccaggga cgaagcgaga cgatgggtgg tgctgaggct gaggccggtc tgtgcggcga
     6361 tttcggcgac cgtgcgcgca ccgtccgcga tcgcctcgag aatgtcgagt ccccgcagca
     6421 tggtctgccc cttcgtgggg gcatcggcgt tcagggcgcg tgatttcgag gaagtcatgc
     6481 tgcgtcgacg tggcgtgggt cgggtggagg aggaaggcag cacggcaccg caagcgcctg
     6541 cacttggcga aattgttgca caccggtgca gccgccgctc gattcgagcg ccccgcccga
     6601 ggggttgccg tcgcacggcg cgctcagttg cccttcagct gccgcgccgt cagcgaagga
     6661 ttgtcgccac tgtacgcggc cttgatcgcg tcccaggctt cctcggcggt ctcggcgtag
     6721 tggaacaggt tcttgtcctc ggggctgatc acgccgtgct cgaccagcac gtcgaagtcg
     6781 atcaggcgct gccagaagtc gcgcccgatc agcacgatcg ggcggcggcg gatcttgcgc
     6841 gtctgcgtca gcgtcagcac ctcgaagagt tcgtcgagcg tgcccagccc gccggggaag
     6901 ctcaccagcg ccaccgcgcg catcaggaag tgcatcttgc ggatcgcgaa gtagtggaac
     6961 tggaagcaca gctcgggcgt gatgtagggg ttgggcgcct cctcgaaggg caggaagatg
     7021 ctcatcccca tgctgcgccc gccggcctcg aaggcgccgc ggttgcccgc ctccatgatc
     7081 cccggaccgc cgccggtggc gacgatcacc ggctcgccga gcgcctcgga ctcgcgcgtg
     7141 accagctcgc cgaagcggcg cgcctcctcg taccaacgcg cgttcttcac catctgacgc
     7201 gcgcgcgcgg tcgccgcctc gtcgccgctc gccagcgcgt cgcgcagcat cgcctcggcc
     7261 acgtccggcg ccttgaagcg cgcgctgccg aacaccacca cggtcgactc caccccctgc
     7321 tcctgctgca ccagctcggc cttgagcagc tcgagctgca gccgcaccgg gcgcaactcg
     7381 tcgcgcagca ggaactcggt gtcggtgaag gccatgcgga aggagctgcc ggggccgtcg
     7441 tagcggccct gcggctgcgc ggccttggcc tcgtcttcgg cagaggggaa gttgcgggcg
     7501 cgcagggggg aattcttgct catcggtcaa tccatccggt tcggggagcc gcatcatcgc
     7561 atccgcgcgt gacgggacgc gagccctgtc cccggttcag cgcccgtccc agcacgcgac
     7621 ggacgcggtc gacaccccct gttccagcgc ggctcgcgtc tgggcctcca gtgcgcgcag
     7681 gaagcccggc cgcgcctgca gccgctgcca gtacgccagg gtcgccggct tgaagcggtc
     7741 ctgcaggccg atgtccgtgg ccagcatcaa ggcgtagccg accgcgacgt cggccgcggt
     7801 gaatcgcccc gcgcacagga actcgcgggt ttcgagccgg ggttcgagcg tgcgcagacg
     7861 cgacaggaac cagcgcgtgt agtcctcggc cgcctgcggc aggcggcgct cgggcggctc
     7921 gaagcggcca tagcgcagga tcagggtttg cggaaaggtc agcgtggctt cgccgaagtg
     7981 caggtagttg aggtaggcgc cgaaatccgc ttcgtccaca ccgacatcga gcgcgcgcgg
     8041 cgaatggcgc gccgccaggt actggcagat cgccgccgac tcgctcatcc acacgtcgtc
     8101 ctccaggaag gcgggaatgg tgccgagcgg attcaccgcg aggtactcct cgtcgcggct
     8161 gcgcggcgga aagggcagca ctttcagcgc gtagggcagc ccgagctctt ccagcatcca
     8221 cagcgcgcgg aaggatctgg cattgatgca gtgataaagg gtcgtcgtca tcggcgttgc
     8281 gctccggaga aaagcagtca cagccccgca ctcgggatgt gggaaaggat tgaaacgatg
     8341 gcaggaactc aacccagcag cgccaccgac ttgatctgcg cccacatccg ccgccccggg
     8401 cgcaacccga gctgctcggc cgcgcggcgg gtgatgcgcg ccagcagcgc ggtgccgccc
     8461 gcgtccaggc gcaccagcac gtgggcgggg gtgtcggcac cgacgacctc ctcgacgatc
     8521 accggcagca ggttctggat gctgctgccc tcgaccaggt ggtcggcgag gctgacatcg
     8581 cgcgcgtgga tgcggaaacg caggcggcgg ccgggcggcg cgggctggcg ctgcacccac
     8641 accgcgccgc cggggaagtc cagccgggtg aggtgatagt ggtcgtcgtg ctcggccacc
     8701 gtgccgtcga ccaccacgcc ggcgtcctcg gtgaaggcgg tgggcaggtc gagccgcgcc
     8761 atggtggcgt gcagctcgcc cgcggccatc gccttgccct cgccgagcat gaccacgtgg
     8821 tcggccagac gcacgacctc gtcgggcgcg tggctgacgt agagcaccgg gatgtcgagc
     8881 tcgtcgtgca ggcgttcgag gtagggcagg atctcctgct tgcgacggtg gtcgagcgcg
     8941 gcgagcggct cgtccatgag caggatgcgc gggctggtga gcagcgcgcg cgccatgccg
     9001 acgcgctggc gctcgccacc ggagaggcgg tcgggcatgc gttcgagcag ggcctcgatg
     9061 ccgagcaggg ccaccgcatg gtcgagcggc acgcggcgcg cggcggccgg gatgcggtgc
     9121 aggccgaact ccaggttgcg ccgcaccgac aggtggggga acaggctggc ctcctggaag
     9181 acgtagccga gcgcgcggcg gtgggtgggc acgcggatgc gacgcgcgtc gtcctgccag
     9241 cactcgccat tgaccaccag gcgaccgcgc gccgccggct ccagcccggc gacgcagcgc
     9301 agcagcgtgg tcttgcccga gccggagtgg ccgaacagcg cggtgacg
//
LOCUS       DAOQNN010000088         9333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3604_length_9333_cov_7.495042, whole genome shotgun sequence.
ACCESSION   DAOQNN010000088 DAOQNN010000000
VERSION     DAOQNN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3604_length_9333_cov_7.495042"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..963
                     /locus_tag="PKV97_06770"
     CDS             106..963
                     /locus_tag="PKV97_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HNB05617.1"
                     /translation="MTYDTHRDLIAMHETLVLADTDLRALAEARVLLERPSLAARLSS
                     FVGSPLEKGMARLPASWRERIGGLAHDALMKAMDTAAKTLQPAAQPASPRLHKLLGSV
                     SGAGGGAFGVAGLTVEIPLSTVLIMRSILDIARAEGEDIGSAQARLAALEVFALGGNA
                     SSDDAAEAGYYAVRAALATTVSEAARHFAQKGLSSEGAPALARLITMVAARYKVQLTQ
                     KAAGMLVPGIGAAAGATINLMFMDHFQAVSRGHFTVRRLERRYGEEAVRAAWQAVGRP
                     HPGNRSGFP"
     assembly_gap    1063..1162
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1420..3987)
                     /locus_tag="PKV97_06775"
     CDS             complement(1420..3987)
                     /locus_tag="PKV97_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB05618.1"
                     /translation="MNRPESDRLLSEEAFSMPGNDETRVGEIIGRNLLECAPDEPLHE
                     AARRMSERRVSSILVVEDDRVVGIWTERDALRVDFDDPTTFALPVRAVMSSPVRVVST
                     ATPLHELTVRFREEHVRHYVVEDETGRRVGIVSQTDVVLNQGIEHYLKLRRVDALVKG
                     GLHALPAAAGLGEATRRMREGGVDAVVVDYGAADASAPAGRYGILTERDVTRLVAQRT
                     ADRALGELASRPLVTCRADDSLYRVRMLLAERHIRHIGVLDAEGELVDLVSFKDILSG
                     MELAYVHELHDALRARDLALSASQRDLYLAEKVIESSLEGVMVTDAHARILSVNPAFT
                     RMTGYAPEEVVGLNPSVLNSGRQSPAFYARMWDGLLGDGQWQGEVWNRRKTGEVYPQL
                     LHITAIRDREGVLTHYAALFTDISRLKETEARIRDLAYYDPLTGLPNRRLLDDRLQVE
                     LAHAARLRCRLAVMFVDLDRFKRINDSLGHAVGDKLLVEIAARLRASLRDDDTVARMG
                     GDEFLVVLNNLSGPDEAATMARRMVAELRRPVNVEGRELVVTTSVGVAVYPDDSRDAD
                     TLVKHADVAMYRAKDEGRNSFQLFEPAMNARSLERLALETALHRALPRDELQLYFQPV
                     MKAEGGELVAVEALLRWRHPDLGLVSPADFIPLAEDTGLIVPIGEWVLRSACEHHRRW
                     CEAGGAPLHMMVNISARQFREEGFAAMVGSVLAETGMTPGDLTLELTESMLMDDTDRA
                     LARLDHLRALGVCLAIDDFGTGYSSLAYLKRFPIDELKIDRLFVRGIDRNTRDAALVS
                     AIVSLGHSLGLRVVAEGVETAAHLDVLRRQGCDLAQGFHFSPPLPWEEFVKAYPPAG"
     gene            4150..5346
                     /locus_tag="PKV97_06780"
     CDS             4150..5346
                     /locus_tag="PKV97_06780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB05619.1"
                     /translation="MIRVLMVSDVFFPRVNGVSTSIATFRRALAAQGVEVRLIAPRYA
                     GEAEEPGIVRVAGRRLPLDPEDRLVDARAMHAAALREAAVCDLVHIQTPFLAHRAGLR
                     AARAQGRPVLLTYHTFFEEYLHHYLPFLPAGVLRGLARRVSRTQCNAVDAVVVPSTAM
                     HERLAGYGVRSPMEVLPTGIPLQDFAAGDGPHFRRLYDIPEDAPMALYIGRVAHEKNI
                     GFLLEVATRLKARRPDFVLVVAGEGPANARLQRQALAAGLGSTVRFVGYLERRRRLPD
                     CYAAADVFVFASRTETQGLVLLEAMAAGLPVVALSAMGTTDILRSERGARIAPDHPDG
                     FAAVVDVVLADPRLRRRLAAEARDYAAEWADGVMAGRLAALYRRLLAAPQSVPPVHAS
                     QVVSTR"
     gene            5424..5627
                     /locus_tag="PKV97_06785"
     CDS             5424..5627
                     /locus_tag="PKV97_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNB05620.1"
                     /translation="MNTTTLTITGMRDEECLRLVTNAIQDLPGIGWLEISLATGAATI
                     EHGAFVAEADIHQAIEDAGFGVR"
     gene            5701..>7200
                     /locus_tag="PKV97_06790"
     CDS             5701..>7200
                     /locus_tag="PKV97_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNB05621.1"
                     /translation="MTPTLRSFPGGLAFVDIETTGGPAQRESITEIGIVQVDEDGVRE
                     WSTLVRPASRIPETIQRLTGIDDDMVADAPRFEDIADEVFDRLDGRLFVAHNARFDHG
                     HLRAAFRRAGLDMRPQVLCTVKLSRRLFPDHRRHGLDHLIERHGLAVADRHRALGDAR
                     LLWQFWQKIHERFPPGHIDAAVRELIGHPSLPPHLDPEQIADLPDTPGVYLFYGERGG
                     ESSQLGGNDEADAEAEADPLGPGRARTGARDRKRHAPLQDLPLYIGKSTRLRSRVLSH
                     FAADHSSDRELSLSQQVRRIEWIATAGEIGALLKEAELVKRLQPTHNRQLRRNRELCT
                     WRLATDIVGDWRLELVHAADLDFGRRDDLYGFFRTRREATNRLRALARDHALCPPLLG
                     LEKPPQGARCFDFQLKRCRGACHGSESPQAHALRLIEALHALKVEHWTWPGPIGLREG
                     AAIHVVDGWRWLGTATDEAMLADLLEAGRPAFDHDIYKILVKAVRRLPVV"
     assembly_gap    7201..7300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7301..8179
                     /locus_tag="PKV97_06795"
     CDS             <7301..8179
                     /locus_tag="PKV97_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB05622.1"
                     /translation="LAVVVFWSGFNLVSRFGATTSFSPFDIAAMRYGVSGMIALPFFL
                     RFVPMRDWPRHAVLACVGGLAYGLCVYWGFAFAPSAHAGVFVNGGIPFWTIVIMAVTS
                     GFRIARPTVVALLLSTAGLLLIGFESLLAPAQGSAWIGDLLFLAAALAWAIFGLLMRR
                     WQVKPQFGILGIASFSALVFMPIYLLWLPSGIGAASWGEIALQCVYQGVIASMLAAGF
                     YSYAVQKVGAGEASMMLALVPAFTAIGAYLILDEALGTTTIVGIVVVSAGALLGALPP
                     AALARVRGSGSAHSAR"
     gene            complement(8218..9333)
                     /locus_tag="PKV97_06800"
                     /pseudo
     CDS             complement(8218..9333)
                     /locus_tag="PKV97_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DASH family cryptochrome"
BASE COUNT         1252 a         3406 c         3174 g         1301 t
ORIGIN      
        1 cgacggtcgc cgccgcggcc tggtcgacga ccgcatcgcc cacgccgcta cctccttcgc
       61 gcgatcctcg ggaacacgcg gcgctgtggc agcgtctgaa accgcatgac gtacgacacc
      121 caccgggacc tcatcgcgat gcacgagacc ctcgtccttg ccgacaccga cctgcgcgcg
      181 ctcgccgagg cgcgcgtgct gctcgagcgg cccagcctgg ccgcgcgcct gagcagcttc
      241 gtcggcagcc cgctggagaa gggcatggcg cgcctgccgg cgagctggcg cgagcgcatc
      301 ggcgggctcg cccacgacgc gctgatgaag gcgatggaca ccgcggcgaa gaccctgcag
      361 cccgccgccc agccagcctc tccgcgcctg cacaagctgc tcggctcggt gagcggcgcg
      421 ggcggcggcg ccttcggggt cgccgggctg acggtggaga tcccgctgtc gaccgtgctg
      481 atcatgcgct ccatcctcga catcgcgcgc gccgagggcg aggacatcgg cagcgcgcag
      541 gcgaggctgg ccgcgctcga ggtgttcgcc ctcggcggca acgcgagcag cgacgacgcg
      601 gccgaggcgg gttactacgc cgtgcgcgcg gccctcgcca ccacggtatc ggaggccgcg
      661 cgccacttcg cgcagaaggg gctgtcgagc gagggcgcgc cggcgctggc acggctgatc
      721 acgatggtgg cggcgcgcta caaggtgcag ctcacccaga aggccgccgg catgctcgtg
      781 ccgggcatcg gcgccgccgc gggggcgacg atcaacctga tgttcatgga ccacttccag
      841 gccgtcagcc gcggccactt caccgtgcgg cggctggaac gccgctacgg cgaggaggcg
      901 gtgcgcgcgg cgtggcaggc ggtcggacgc ccccaccccg gaaaccgtag cggtttcccg
      961 taggagcgac gcaagtcgcg atttggccgt acgaagaccg cggcgcctgg attgcggccg
     1021 atcgcgactg gcgtcgctcc tacgagaacc gcagcgccgc gcnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnggccgatc gcgactggcg tcgctcctac gagaaccgca
     1201 gcgccgcgct ccgggacaac cgcccccggc gcagccccgg taggagcgac gcaagtcgcg
     1261 atttggccgt acgaagaccg cggcgcgtgg attgcggccg atcgcgactg gcgtcgctcc
     1321 tacgagaacc gcagcgccgc gctccgggac gaccgccccc ggtgcagccc cggtaggagc
     1381 gacgcaagtc gcgattcggg cgggcgagga ccgcggcgct cagcccgccg gcgggtaggc
     1441 tttgacgaac tcttcccagg gcaagggcgg gctgaagtgg aagccttgcg ccaggtcgca
     1501 gccctggcgg cgcagcacgt cgaggtgggc ggcggtctcg acgccctcgg cgaccacgcg
     1561 caggcccagg ctgtggccga gcgagacgat cgccgaaacc agcgccgcgt cgcgcgtgtt
     1621 gcggtcgatg ccgcgcacga acaggcggtc gatcttgagt tcgtcgatcg ggaagcgctt
     1681 gagataggcg agcgacgaat accccgtgcc gaaatcgtcg atcgccaggc acacgccgag
     1741 cgcgcgcaga tggtcgaggc gcgcgagcgc gcggtcggtg tcgtccatca gcatgctttc
     1801 ggtgagctcc agcgtcaggt cgcccggcgt catgccggtc tcggcgagca cgctgccgac
     1861 catcgcggcg aagccttcct cgcggaactg gcgcgccgag atgttcacca tcatgtgcag
     1921 cggtgcgccg cccgcctcgc accagcgccg gtgatgctcg caggcgctgc gcagcaccca
     1981 ttcgccgatc ggcacgatca ggccggtgtc ttcggcgagc gggatgaagt cggccggcga
     2041 caccaggccg aggtcggggt ggcgccagcg cagcaaggcc tcgaccgcga ccagttcgcc
     2101 tccctcggcc ttcatcaccg gctggaagta gagctgcagc tcgtcgcgcg gcagggctcg
     2161 gtgcagcgcg gtctcgagcg cgaggcgctc gagcgagcgc gcgttcatcg ccggctcgaa
     2221 gagctggaag ctgttgcgcc cctcgtcctt ggcgcggtac atcgccacgt ccgcgtgctt
     2281 gaccagggtg tcggcgtcgc gcgagtcgtc cgggtacacg gcgacgccca cgctggtcgt
     2341 caccaccagc tcgcggccct cgacattcac cgggcggcgc agctccgcga ccatcctgcg
     2401 cgccatcgtc gccgcctcgt cggggccgga caggttgttg agcacgacga ggaactcgtc
     2461 gccacccatg cgggcgacgg tgtcgtcgtc gcgcaggctg gcgcgcaggc gggcggcgat
     2521 ctcgaccagc agcttgtcgc ccaccgcgtg gccgaggctg tcgttgatgc gcttgaagcg
     2581 gtcgaggtcg acgaacatca ccgccagccg gcagcgcagg cgtgcggcgt gggcgagctc
     2641 gacctgcagg cggtcgtcga ggaggcggcg gttgggcagg ccggtgagcg ggtcgtaata
     2701 ggcgaggtcg cggatgcgcg cctcggtctc cttgagccgg ctgatgtcgg tgaacagcgc
     2761 cgcgtaatgg gtgagcacgc cctcgcggtc gcggatcgcg gtgatgtgca gcagctgcgg
     2821 atacacctcg ccggtcttgc gccggttcca cacctcgcct tgccactggc cgtcgcccag
     2881 caggccgtcc cacatgcggg cgtagaaggc ggggctctgg cggccggagt tgagcaccga
     2941 gggattgagc ccgacgacct cttccggcgc atatccggtc atgcgcgtga aggcggggtt
     3001 caccgagagg atgcgcgcgt gcgcgtcggt gaccatcacc ccttcgagcg agctctcgat
     3061 caccttctcg gccaggtaga ggtcgcgctg cgaggcgctc agcgcgaggt cgcgcgcgcg
     3121 cagggcgtcg tgcagctcat gcacgtacgc cagctccatg ccgctgagga tgtccttgaa
     3181 gctgaccagg tcgaccagtt cgccttcggc gtcgagcacg ccgatgtggc ggatgtggcg
     3241 ctcggcgagc agcatgcgca cgcggtacag gctgtcgtcg gcgcggcagg tgacgagcgg
     3301 ccggctggcg agctcgccga gcgcgcggtc ggccgtgcgc tgcgccacca gccgggtgac
     3361 gtcgcgctcg gtgaggatgc cgtagcgccc cgcgggcgcg gaggcgtccg ccgcgccgta
     3421 atccaccacg accgcgtcca ccccgccctc gcgcatgcgc cgcgtcgcct cgcccagccc
     3481 cgccgccgcg ggcagcgcat gcaggccgcc cttgaccagc gcatccaccc gacgcagctt
     3541 gaggtagtgc tcgatgccct ggttcagcac cacgtcggtc tgcgacacga tgccaacgcg
     3601 ccggccggtc tcgtcttcga ccacgtagtg gcgcacatgc tcctcgcgga agcgcacggt
     3661 cagctcgtgc agcggcgtcg ccgtcgacac cacgcgcaca ggcgagctca tcacggcgcg
     3721 taccggcagg gcgaaggtcg tggggtcgtc gaaatccacc cgcaacgcgt cgcgctcggt
     3781 ccagatgccc accacacggt cgtcctcgac caccaggatc gagctcaccc ggcgctcgct
     3841 catgcgccgc gcggcctcgt gcaggggctc gtccggggcg cactcgagca ggttgcggcc
     3901 gatgatctcg ccgacgcggg tctcgtcgtt gccgggcatg gagaaggcct cctcgctcag
     3961 gaggcggtcg gattcaggac ggttcatggg ggactcggca caggctttag attctgtata
     4021 acggctccag ccgactgaaa cctgaggcgg ctgcgcacat tgtcgcaccc gcggccctgc
     4081 aggcggaccc cggctaaccc gcagggaggg cttcaccctg cggtaacacg cccggcgcat
     4141 gctggccgaa tgatccgcgt gctgatggtc tccgacgtct tctttccgcg tgtgaacggc
     4201 gtgtccacgt cgatcgcgac ctttcgccgc gcgcttgccg cgcagggcgt cgaggtgcgc
     4261 ctgatcgcgc cgcgctacgc cggcgaggcc gaggagcccg gcatcgtgcg cgtcgcgggg
     4321 cgccgcctgc cgctcgaccc cgaggaccgc ctggtcgatg cgcgcgcgat gcatgccgcc
     4381 gccctgcgcg aggccgccgt ctgcgacctg gtgcacatcc agaccccctt cctcgcccat
     4441 cgtgccggac tgcgcgccgc gcgcgcccag ggcaggccgg tactgctgac ctatcacacc
     4501 ttcttcgagg aatacctcca ccactacctg cccttcctgc ccgcgggcgt gctgcgcggg
     4561 ctggcgcggc gggtgtcgcg cacgcagtgc aatgcggtgg atgcggtggt ggtgccctcg
     4621 accgcgatgc acgagcgcct cgccggctat ggcgtgcgca gcccgatgga ggtcctgccc
     4681 accggcatcc cgctgcagga cttcgccgcc ggcgacggtc cgcacttccg ccgcctctac
     4741 gacatcccgg aagatgcgcc gatggcgctc tacatcggcc gcgtcgcgca cgagaagaac
     4801 atcggcttcc tgctcgaggt cgccacccgg ctgaaggcgc gccgccccga cttcgtcctc
     4861 gtggtcgccg gcgaggggcc ggcgaacgcc cgcctgcagc gccaggcgct cgccgccggg
     4921 ttgggcagca ccgtgcgctt cgtcggttat ctcgagcgcc gtcgccgcct gcccgactgc
     4981 tacgccgccg ccgacgtgtt cgtgttcgcc tcgcgcaccg agacgcaggg cctggtgctg
     5041 ctcgaggcga tggccgccgg gctgccggtg gtcgcgctgt ccgcgatggg caccaccgac
     5101 atcctgcgct ccgagcgcgg cgcacgcatc gcgcccgacc atcccgacgg cttcgccgcc
     5161 gtggtcgacg tcgtgctcgc cgacccccgc ctgcgtcggc gcctcgccgc cgaggcgcgc
     5221 gactacgccg cggagtgggc cgacggcgtg atggccgggc ggctcgccgc gctctaccgc
     5281 aggctgctcg ccgcgccgca gtctgtgccg ccggtgcacg cgtcgcaggt cgtctccacc
     5341 cgctgagacg cttggcaggc ggcccgggct tgcgcgcgtc cgtgcctgcg cacaagatgc
     5401 gccgatcgga cacggagctg cccatgaaca ccaccacgct cacgatcacc ggcatgcgcg
     5461 acgaggagtg cctgcgcctg gtgaccaatg ccatccagga cctgcccggc atcggctggc
     5521 tggagatctc gctcgcgacc ggcgcggcca ccatcgagca cggcgccttc gtcgccgagg
     5581 ccgacatcca ccaggccatc gaggacgccg gcttcggcgt gcgctgaaca cggcgggcac
     5641 gggaacccgc gcgcccgcgc acgctcgaag agcgacccca tccgccggtt cgcccccctc
     5701 ttgacgccca ccctccgcag ctttcccggc ggcctcgcct ttgtcgacat cgagaccacc
     5761 ggcggcccag cccaacgcga atccatcacc gagatcggca tcgtccaggt ggacgaggac
     5821 ggcgtgcgcg agtggtcgac gctggtgcgt cccgcgtcgc gcatccccga gaccatccag
     5881 cgcctcaccg gcatcgacga cgacatggtc gccgacgcac cgcgcttcga ggacatcgcc
     5941 gacgaggtct tcgaccgtct cgacggccgc ctcttcgtcg cccacaacgc acgcttcgac
     6001 cacggccacc tgcgcgccgc cttccgccgc gccgggctcg acatgcggcc gcaggtgctg
     6061 tgcaccgtca agctgtcgcg ccggctgttc cccgaccacc gccgccacgg tctcgaccac
     6121 ctcatcgagc gccacggcct ggcggtggcc gaccgccacc gtgcgctcgg cgacgcccgg
     6181 ctgctgtggc agttctggca gaagatccac gaacgctttc cgcccggtca catcgatgcc
     6241 gcggtgcgcg aactcatcgg ccaccccagc ctgccccccc acctcgaccc cgagcaaatc
     6301 gccgacctgc ccgacacgcc gggggtgtat ctgttctacg gcgagcgcgg gggcgagagc
     6361 agccagcttg ggggcaacga tgaagccgac gccgaagccg aggccgatcc gctcggaccc
     6421 ggcagggcgc gcaccggcgc gcgcgaccgc aagcgccacg cgccgctgca ggacttgccg
     6481 ctctacatcg gcaagagcac gcggctgcgc agccgcgtgc tgtcgcactt cgccgccgac
     6541 cacagcagcg accgcgagct cagcctcagc cagcaggtgc gccgcatcga atggatcgcg
     6601 accgccggcg agatcggcgc gctgctgaag gaagccgaac tggtcaagcg cctgcagccc
     6661 acccacaacc gccagctgcg ccgcaaccgc gagctgtgca cctggcggct cgccaccgac
     6721 atcgtcggcg actggcggct ggagctggtg catgcggccg acctcgactt cggccgccgc
     6781 gacgacctct acggtttctt ccgcacccgc cgcgaggcca ccaaccggct gcgcgcgctc
     6841 gcccgcgacc acgccctgtg cccgccgctg ctcggcctgg agaaaccccc gcaaggtgcg
     6901 cgctgcttcg acttccagtt gaagcgctgc cgcggcgcct gccacggcag cgaatccccc
     6961 caggcccacg ccctgcgcct gatcgaggcc ctgcacgcgc tgaaggtcga gcactggacc
     7021 tggcccggcc cgatcggcct gcgcgagggc gcggccatcc acgtcgtcga cggctggcgc
     7081 tggctcggca ccgccaccga cgaagccatg ctcgccgacc tgctggaggc cggccgcccg
     7141 gccttcgacc acgacatcta caagatcctg gtcaaggcgg tgaggcggct gccggtggtg
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctcgccgtgg tggtgttctg
     7321 gtccggcttc aacctcgtgt cgcgcttcgg cgccaccacc agcttcagcc ccttcgacat
     7381 cgccgcgatg cgctacggcg tctccggcat gatcgcgctg cccttcttcc tgcgcttcgt
     7441 gccgatgcgc gactggccgc gccatgccgt gctcgcctgc gtcggcgggc tggcctacgg
     7501 gctgtgcgtg tattggggct tcgccttcgc gccgagcgcg cacgcggggg ttttcgtgaa
     7561 cggcggcatc ccgttctgga ccatcgtgat catggcggtg acgagcggct tccgcatcgc
     7621 ccgcccgacc gtggtcgccc tgctgctctc gaccgccggc ctgctgctga tcggcttcga
     7681 gagcctgctc gccccggcgc aggggagcgc ctggatcggc gacctgctct tcctcgccgc
     7741 cgcactggcg tgggcgatct tcggcctgct gatgcggcgc tggcaggtca agccgcagtt
     7801 cggcatcctc ggcatcgcga gcttctcggc gctggtcttc atgccgatct acctgctctg
     7861 gctgccctcc ggcatcggcg ccgcgagctg gggcgagatc gcgctgcagt gcgtgtacca
     7921 gggcgtgatc gcctccatgc tcgccgccgg cttctacagc tacgcggtgc agaaggtcgg
     7981 cgccggcgaa gcctcgatga tgctggcgct ggtgcccgcg tttaccgcga tcggcgccta
     8041 tctgatcctg gacgaggccc tcggcacgac caccatcgtc ggcatcgtgg tggtgtcggc
     8101 cggggccctg ctcggcgcgc tgccgccagc ggcgctggcc cgcgtgcgcg gctcgggctc
     8161 ggcccacagc gcccgatagg cgccgtccgc gtcgtacatc gccgcctgcc ggtccggatc
     8221 gaagcgccgc ccgccacgcg gatcggtgcc gcggccggcg atgtagagcc agttgccctg
     8281 gttgctatac acgtcgtaat cgaccagctg cgcctcgaac cacgccgcgc cggcacgcca
     8341 gtcacagccg aggtcgtgga tcaggtagct ggcgacgatc tgacgcatgc ggttcgacag
     8401 ccagccggtg gccgcgagct cgcgcatgcc ggcgtcgatg aagggatggc cggtgccgcc
     8461 cacgcgccag gccgcgaacg caacggcgtc gtgcgcgggc tgcgctgcgc cttcgttcag
     8521 cccgcgcgcc cggtacagac ggcggccgtg ctgcagatgc aggaggcgga agtagtcgcg
     8581 ccacagcagc tcgaaccaca gccagtagct tccgtcgctc ggcccctgct cgatctcgtg
     8641 gcggcgcagc gcggccaagg ccacccgcgc cgacaaggcg ccctgcgcca gccagggcga
     8701 gaacttgctg gagaagtcga cgccgctcag cccgttgcgc gtcgccttgt agtgccgcgg
     8761 ccgatcgccg gcgaaatagc gtgccagatg cgcgagcgcc gcacgctcgc ccccggcgaa
     8821 tgccggctgc caccacggaa acgaggcctg ttcgccgatt tccacggtga gcgcatcggc
     8881 ggccccgcag gagcccccgt aggagcgacg caagtcgcga ttccccggtt ccgcacgctc
     8941 cacccccggc gagcgcccgg cgcggagatc gcgacttgcg tcgctcctgc gggtcggtcg
     9001 atcgagcggt ggccacggcg gcagggtccg cggcgccggc aggggcgcgg gcggctgcag
     9061 gccggcggac tccactgcct ggcgaaaggc ggtgaacacc ctgggcaggc gcgccgccgc
     9121 gaaaggcagc gccgcggggt cgagcagggt ggactgccat atcgccttca ccttcagccc
     9181 ggcggcgcgt agcgcggcga gttcgtcctc ctcctcgggt gccgcgatcg cctcgcaaac
     9241 cactacggcc gcccccacgg cttccgcgag cgccggcagc accgcgcgcg gcgcgccgtt
     9301 cagctgcagc agcgcgctgc cgcgcgcccg cag
//
LOCUS       DAOQNN010000089         9283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3636_length_9283_cov_8.778825, whole genome shotgun sequence.
ACCESSION   DAOQNN010000089 DAOQNN010000000
VERSION     DAOQNN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9283
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3636_length_9283_cov_8.778825"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="PKV97_06805"
     CDS             complement(<1..1212)
                     /locus_tag="PKV97_06805"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB05623.1"
                     /translation="MNFENLGLAEELLKAVASTGYTTPTPVQQRAIPAAIAGRDLLVS
                     SHTGSGKTAAFTLPALNRIIDRRPAPGSGPRVLVLTPTRELAQQVEKAVQTYGKALRW
                     LNTACLVGGAPMFAQIKQLQRQCDVVVATPGRLLDHLTRRKIKLSDVEVLILDEADRM
                     LDMGFAEDIDAIVAATPAKRQTLLFSATLDGVVGSMATRMTRDAERIEIEVAQEDRGQ
                     IEQRLMFADDLGHKSRLLHALLGEDGMNQAVVFTATKRSADELSLALQEKGISAAALH
                     GDMHQTQRNRTLDRLRQGRIGVLVATDVAARGIDVAGISHVINFDPPRQAEDYVHRIG
                     RTGRAGRDGIAITLSGPRETGLIRAIERFTGDRLAVHTIPGMEPSPRKPAGPRPGGNS
                     GRRFGSGAKPSG"
     gene            complement(1743..2021)
                     /locus_tag="PKV97_06810"
                     /pseudo
     CDS             complement(1743..2021)
                     /locus_tag="PKV97_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            2239..3414
                     /locus_tag="PKV97_06815"
     CDS             2239..3414
                     /locus_tag="PKV97_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05624.1"
                     /translation="MSTQDSTGEDPIRVLATHAAGQVLAVSLHIPDRAWMGGAVIGMA
                     PAEGVEHLRRHASHSPQAHAHALAHACAPAAGEPPPERAEQLRTERRLAAEMIDMHLR
                     RLLLDWPPSLGFEPRINRYAEFHRRLLHAGEHDNDFVLGGEVLDLVARELLAGFFNQI
                     RMPHGIGEFLDRLAGGGSLSSILGELIAHGPSTVPPEKRTALLGTLSADAWAAAVGTW
                     PSAQFIRHPRYAGEPAETGPLARHAASPLVRILLERGNRISARLFAKAIDVADCASTM
                     RHPGSAEVAPLIDACRVAPDVGLARVISARGVLLCWVRLAEGRIADCAIVPAGAWNLE
                     PDGVYCREAMCLQVTDGDPEALKKRLEWLMLAIDPSLPFVVELVAAGEGAGVAPASA"
     gene            complement(3448..3948)
                     /locus_tag="PKV97_06820"
     CDS             complement(3448..3948)
                     /locus_tag="PKV97_06820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNB05625.1"
                     /translation="MTQQQASGEDFQRHFRNALGMFATGITVVTTRTPAGEPIGLTVN
                     SFNSVSLDPPLIVWSLANELPSRTLFEGCEYYAINVLAEDQADLSQRFASRLGEKFAG
                     LEFDSGVGGVPLLAGCCARFQCRNTVRHAGGDHVVFISEVVDFDRAEERAPLLYFGGA
                     YRQLRA"
     gene            4060..5139
                     /gene="mutY"
                     /locus_tag="PKV97_06825"
     CDS             4060..5139
                     /gene="mutY"
                     /locus_tag="PKV97_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNB05626.1"
                     /translation="MSDFSIRLIEWQRKHGRHDLPWQGGHDPYRIWLSEIMLQQTRVE
                     TVIPYYERFLARFPDVAALAAAPVEDVMALWSGLGYYARARNLHRAARVVMDAHGGAF
                     PRSAAAIAGLPGIGRSTAAAIAAFAWGERAAILDGNVKRVLCRVFGIEGFPGDKAVET
                     RLWALAESLLPERGIGRYIQAQMDLGATLCTRARPACARCPFHDDCVARRDGRVAALP
                     TARPKKVVPRRGARCAVILHQGAVLLERRPPAGIWGGLLALPELPAEVDDAQAWSAQR
                     FGLATAAPRPLAPLTHAFTHFVLELQPLLLHASAIQGLADDGALCWLPLGAHAEAALP
                     APVRRILDVLAAPGLFDEGAPARGA"
     gene            complement(5184..5786)
                     /locus_tag="PKV97_06830"
     CDS             complement(5184..5786)
                     /locus_tag="PKV97_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNB05627.1"
                     /translation="MTPPPDTLPLFPLKTVLFPGGVLPLRVFEPRYMDMVTRCMREGG
                     SFGVCLIAAGDEVGEAAVPHPVGTEALIERWDMEQPGVLELLVRGGRRFRIVDHELER
                     DGLLVGSVRWLEEPPAEPVPAAQAELLPLLRSIVEELGDRLPPPHDFDDAAWVGARYI
                     ELLPIPLLAKQKLLELDDIVSRLEILQQFLREHGLMPKKA"
     gene            complement(5828..6454)
                     /locus_tag="PKV97_06835"
     CDS             complement(5828..6454)
                     /locus_tag="PKV97_06835"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HNB05628.1"
                     /translation="MTPPRTITVAFTGASGLPYGVRLVECLLQAGCRVWLLYSQVAQI
                     VARQEMDWNLPARPAEVEAELSARFAAAPGQLRVFGREEWFAPPASGSNPPDAMVVCP
                     CTVATLASIAGGLSQNLIERAADVVIKEGRKLVLVPRETPYSAIHLENMLKLARLGVC
                     ILPPNPGFYHHPQTVQDLVDFVVARILDQLGVPHSLMARWGEDREQAG"
     gene            complement(6605..>6848)
                     /locus_tag="PKV97_06840"
     CDS             complement(6605..>6848)
                     /locus_tag="PKV97_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05629.1"
                     /translation="SRLASLLPERHPDRGGFPAAFHGYWDAAAELPGAVPVFVGATQV
                     AIPPHGIRVDAALPVQSRLASLPLESCAVCEPTMTH"
     assembly_gap    6849..6948
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7013..8179)
                     /locus_tag="PKV97_06845"
     CDS             complement(7013..8179)
                     /locus_tag="PKV97_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB05630.1"
                     /translation="MSPLRRLALALASSLSLACALPAVAAGVSADEIVVGTVSDLSGP
                     IAMLGVPVRDGMLMRFDEANAGGGVHGRKIRLAVEDAGYDPKRAVLAARKLVQHDQAF
                     AFIANMGTPVVMASMPIIVDAGRLHLFPFSPHRATYEPLHPLKFQNFAPYQDYMEAAT
                     RHMVRERGYQRSCLLYQDDDYGLEVMKGVEKALAGLGTELVERTSYKRGATDFSSQIA
                     RLRAARCDLVVLATVVRETVAAMSEARKIGWDVDMLVTASGYSAQTHELGGAAVEGLY
                     GVSVLPHPYAEGANSQLAAWIERYRARFKAEPNVWSVMGYTLADLFVRTAEATGRELT
                     PERFAHTLESMAFTRDYFGSPAYRFSTDDHLGNRKGRLAQIRNGRWELITDYLE"
     gene            complement(8292..8516)
                     /locus_tag="PKV97_06850"
     CDS             complement(8292..8516)
                     /locus_tag="PKV97_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05631.1"
                     /translation="MSAWSCPHDLDGICQRVRGAICSPGMRGCELEGKVRFALDELNE
                     PRKPVKARPAETRPEPKPGAAAAPRRRLPF"
     gene            complement(8611..>9283)
                     /locus_tag="PKV97_06855"
     CDS             complement(8611..>9283)
                     /locus_tag="PKV97_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB05632.1"
                     /translation="GALASLPARRLGGLYFGMSTLAFALIVEEALARWESLTHGAAGL
                     TVGAFTLAGWRADSTLAQATVSVAVLALAMLACARLFASRHGRAWRAVRDDEIAAAAC
                     GIDNDAAKMQAFVVGGALSGLAGGLYAHWIGFISPEQFGLVFSFELLMLAFIGGARRL
                     AGAAWGALVIVAIPPLISLLRDRLPGDWARASGLELLLFGAVIVGVVLLRPAGLAGET
                     KRHDR"
BASE COUNT         1302 a         3140 c         3436 g         1305 t
ORIGIN      
        1 gccagagggc ttggcaccgc tgccgaagcg gcgaccgctg ttgccgcccg ggcgcgggcc
       61 ggccggcttg cgcggcgagg gctccatgcc ggggatggtg tgcaccgcga ggcggtcgcc
      121 ggtgaagcgc tcgatcgcgc ggatgaggcc ggtctcgcgc gggccggaga gcgtgatcgc
      181 gatgccgtcg cggccggcgc ggccggtgcg gccgatgcgg tgcacgtagt cctcggcctg
      241 gcgcggcggg tcgaagttga tcacgtggct gatgccggcg acgtcgatgc cgcgcgcggc
      301 gacgtcggtc gccaccagca cgccgatgcg gccctggcgc aggcggtcga gggtgcggtt
      361 gcgctgggtc tggtgcatgt cgccatgcag cgcggcggcg gagatgccct tttcctgcag
      421 cgcgagcgag agctcgtcgg cgctgcgctt ggtggcggtg aacaccaccg cctggttcat
      481 cccgtcctcg ccgagcaggg cgtggagcag gcggctcttg tggccgaggt cgtcggcgaa
      541 catcaggcgc tgctcgatct ggccgcggtc ttcctgggcg acctcgatct cgatgcgctc
      601 ggcgtcgcgc gtcatgcgcg tcgccatgct gcccaccacg ccgtcgagcg tggccgagaa
      661 cagcagggtc tggcgcttgg ccggggtggc ggcgacgatg gcgtcgatgt cctcggcgaa
      721 gcccatgtcg agcatgcggt cggcctcgtc gaggatcagc acctcgacgt ccgacagctt
      781 gatcttgcgg cgggtcaggt ggtcgagcag gcggcccggg gtggcgacga cgacgtcgca
      841 ctggcgctgc agctgcttga tctgcgcgaa catcggcgcg ccaccgacca ggcaggcggt
      901 gttgagccag cgcagggcct tgccgtaggt ctgcaccgcc ttctcgacct gctgggcgag
      961 ctcgcgggtg ggggtcagca ccagcacgcg cggaccgctg ccgggcgccg gacggcggtc
     1021 gatgatcctg ttcagggcgg gcagggtgaa ggcggcggtc ttgccgctgc cagtgtggct
     1081 ggagacgagc aggtcgcggc cggcgatcgc cgccgggatg gcgcgctgct gcaccggggt
     1141 cggggtggtg tagccggtgc tggcgacggc cttgaggagt tcttcggcga ggccgaggtt
     1201 ttcgaagttc atgcttcttc ctgtcttgct gcccgccgcg cgggaatggg cgggcggagc
     1261 gatccggacc cgccgcaggc gggccgcaat gacatcacgc cgcgccgtgc ctgcggacag
     1321 gaacggcacg agacgtcgca agggcgacgg gacgcgcgat gggcgtggcc ggtcgatccg
     1381 gtggcgcatg gacgcctgga aagaccgcgc atcggcacca accggttgtc acgatgccag
     1441 acgatcggaa gaacgaatgg aaaacccgac ggagtgtcgg cgacgggagg tcggaggcga
     1501 tggggctgtg gttacagtcc ctcgggccgc tccggctcgc gtccctcgac gcgtcggtat
     1561 tgctctcggc cctgttgctg cattgcacaa cgacaggcag tataccggtt ggacggcagg
     1621 ctgtcgcggt ttattttgca cggcccgcct tggacacggc gccggaatgc aagcgggccc
     1681 gctccggcag cagccggaac gggcccgcag gccacggcgc cgagggcgcc gtcggtgtgc
     1741 gcttacttgt tgaccgaggc cttcagcgtg gcgccggcgg tgaacttggg cacgctcgac
     1801 gcggcgatct cgatggtggc gccggtctgc gggttgcggc cggtgcgggc ggcgcgctgc
     1861 gaaacctcga aggagccgaa accggtgaag gccacctttt cgcccttggc gagctgctcg
     1921 gcgatgatgt cgagcacggc ggacagcgcg cggtcggcct gggcgcggtg cacatccaga
     1981 cggtcggcca gggcttcgac gaattcacct ttattcatgc ttttcctcga ttggtgtgtg
     2041 agtgggagtt ccggaaagtc cggtctacgc ggcggattct agcactgtcg cggatcctgg
     2101 ggcgtgcgca gcattgcacg ctcgcccttc ttcctccccg tggcgggcgg cctcgatgca
     2161 catccgtcca cgcgaacaac aaacttcccc gatgcgggta ttctccagcc tgattcaaga
     2221 atgcatacga gcgacgacat gagcacacag gacagcaccg gcgaggatcc gatccgggtt
     2281 ctcgccaccc atgccgcggg tcaggtcctt gcggtgtcgc tgcacatccc cgaccgcgcc
     2341 tggatgggcg gcgcggtgat cggcatggcg cccgcggagg gcgtggagca tctgcgccgc
     2401 cacgccagcc acagcccgca ggcccacgct cacgccctcg cgcacgcctg cgctccggcc
     2461 gcgggcgagc cgccgccgga gcgcgcagag cagctgcgta ccgagcgccg cctggccgcc
     2521 gaaatgatcg acatgcatct gcgccgcctc ctcctcgact ggccgccctc gctcggcttc
     2581 gagccacgca tcaaccgcta tgccgaattc caccgccgcc tgctgcatgc cggggagcac
     2641 gacaacgact tcgtgctcgg cggcgaggtg ctcgacctgg tcgcgcgcga gctgctcgcc
     2701 ggcttcttca accagatccg catgccgcac ggaatcggcg agttcctcga ccgcctcgcg
     2761 ggcggcggca gcctgagctc gatcctgggc gagctgatcg cccacggccc gtccaccgtg
     2821 ccgccggaaa aacgcaccgc gctgctcggc acgctcagcg ccgacgcctg ggccgccgcg
     2881 gtgggcacct ggccgagcgc gcaattcatc cgtcacccgc gctacgccgg cgagcccgcc
     2941 gagactggac cgctcgcgcg ccacgccgcc tcgccgctgg tgcgcatcct gctcgagcgc
     3001 ggcaaccgca tctcggcgcg cctgttcgcc aaggcgatcg acgtggccga ctgcgcgagc
     3061 acgatgcgcc atccgggcag cgcagaggtg gcgccgctga tcgacgcctg ccgcgtcgcc
     3121 ccggatgtcg gtctggcgcg cgtgatcagc gcgcgcggcg tgctgttatg ctgggtccgc
     3181 ctcgccgagg gtcgcatcgc cgactgcgcg atcgtgccgg cgggtgcgtg gaacctcgag
     3241 cccgacggcg tctattgccg cgaggcgatg tgcctgcagg tcacggacgg cgaccccgag
     3301 gcgttgaaga aacggctgga gtggctgatg ctcgcgatcg acccctcgct gcctttcgtg
     3361 gtcgagctgg tcgccgcagg ggaaggcgcg ggcgtggcgc cggcatccgc ctgagcccgg
     3421 accgccgccg cgcccgcggc gcggccttca ggcacgcagc tggcggtagg cgccgccgaa
     3481 gtacagcagc ggcgcgcgct cttcggcgcg gtcgaaatcg accacctcgc tgatgaagac
     3541 cacgtggtcg ccgcccgcgt ggcgcaccgt gttgcggcac tggaagcgcg cgcagcagcc
     3601 tgcgagcagc ggcacgccgc cgacccccga gtcgaactcc agcccggcga acttttcgcc
     3661 gagccggctc gcgaagcgct gcgacagatc ggcctggtcc tcggcgagca cattgatcgc
     3721 gtagtattcg cagccctcga agagcgtgcg gctgggcagc tcgttggcca ggctccacac
     3781 gatcagcggc gggtcgagcg acaccgagtt gaaggagttg accgtaaggc cgatcggctc
     3841 gccggcgggc gtgcgcgtgg tcaccacggt gatgccggtg gcgaacatgc cgagcgcgtt
     3901 gcggaagtgg cgctggaagt cctcgccgga agcttgctgt tgggtcatgg acggacgggc
     3961 gcggtgggcc gcgctgctcg ggtgggagta aagccgggat tatcgccgcg cgcgcgggca
     4021 gcgtcgagcg cgcaggcaac gcaaggaaca aaaggggaca tgagcgattt ttccatccgc
     4081 ctgatcgaat ggcagcgcaa gcacggccgc cacgacctgc cctggcaggg cggccacgac
     4141 ccctaccgca tctggctgtc ggagatcatg ctgcagcaga cccgggtcga gaccgtgatc
     4201 ccctactacg agcgcttcct cgcgcgcttc cccgacgtcg ccgcgctcgc cgcggcgccg
     4261 gtcgaggacg tcatggcgtt gtggagcggc ctgggctact acgcccgcgc gcgcaacctg
     4321 caccgcgcgg cgcgggtggt catggacgcg cacggcggcg cctttccgcg cagcgccgcg
     4381 gcgatcgccg ggctgcccgg catcggtcgc tccaccgcgg cggcgatcgc cgccttcgcc
     4441 tggggcgagc gtgcggcgat cctcgacggc aacgtcaagc gcgtgctgtg ccgcgtcttc
     4501 ggcatcgagg gctttcccgg cgacaaggcg gtggagacgc ggctgtgggc gctcgccgag
     4561 tcgctgctgc cggagcgcgg gatcggccgc tacatccagg cgcagatgga tctcggcgcc
     4621 acgctgtgca cccgcgcccg ccccgcctgc gcgcgctgcc ccttccacga cgactgcgtc
     4681 gcccgtcgcg acgggcgcgt ggccgcgttg ccgaccgcgc gcccgaagaa ggtggtgccg
     4741 cggcgtggcg cgcgctgtgc ggtgatcctg caccagggcg cggtgctgct ggagcgtcgc
     4801 ccgccggcgg ggatctgggg cggcctgctg gcgctgccgg aattgcccgc cgaggtggac
     4861 gacgcccagg cctggagcgc ccagcgtttc ggcctggcca ccgccgcgcc ccggccgctc
     4921 gcaccactca cccacgcctt cacccacttc gtgctcgagc tgcagccgct gctgctgcac
     4981 gccagtgcca tccaaggcct ggccgacgac ggcgcgctgt gctggctgcc gctgggcgcc
     5041 cacgccgagg ccgccctgcc cgcgccggtg cggcgcatcc tcgacgtcct cgcggcgccg
     5101 ggcctcttcg acgagggcgc gcccgcgcgc ggcgcctgag cctgcgggga acgcaccggt
     5161 gcgccgcggc gagtcgtgcg cgctcaggcc ttcttcggca tcaggccgtg ctcgcgcagg
     5221 aactgctgga ggatctccag ccggctgacg atatcgtcga gctcgagcag tttctgcttg
     5281 gcgagcagcg gaatgggcag cagctcgatg tagcgcgcgc ccacccaggc agcgtcgtcg
     5341 aagtcgtgcg gcggcggcag gcggtcgccg agctcctcga cgatcgagcg cagcagcggc
     5401 agaagctcgg cctgcgcggc gggcaccggc tccgcgggcg gctcctccag ccagcgcacg
     5461 ctgcccacca ggaggccgtc gcgctcgagc tcgtggtcga cgatgcggaa gcgccgtccg
     5521 ccgcgcacca gcaactcgag cacgccgggc tgctccatgt cccagcgctc gatcagcgcc
     5581 tcggtgccca ccgggtgcgg cacggcggct tcgccgacct cgtcgccggc cgcgatcagg
     5641 cagacgccga aggagccgcc ctcgcgcatg cagcgcgtga ccatgtccat gtagcgcggc
     5701 tcgaacacgc gcagcggcag tacgccgccg ggaaagagca cggtcttgag cggaaacagc
     5761 gggagggtgt cgggcggcgg ggtcatggga cgctccggtg cgcagggggc agcggtgccg
     5821 gcaggcttca gccggcctgc tcgcgatcct cgccccagcg cgccatcagg ctgtgcggca
     5881 cgccgagctg gtcgaggatg cgggcgacga cgaagtcgac caggtcctgc acggtctgcg
     5941 gatggtgata gaagccgggg ttgggcggca ggatgcacac gccgaggcgg gcgagcttga
     6001 gcatgttctc gagatggatc gccgagtacg gggtctcgcg cggcacgagc acgagcttgc
     6061 ggccttcctt gatgacgacg tcggcggcgc gctcgatgag gttctggctg agcccgccgg
     6121 cgatcgaggc gagggtggcg acggtgcatg ggcacacgac catggcgtcg ggcgggttgg
     6181 agccggaggc cggcggggcg aaccactcct cgcggccgaa cacgcgcaac tgcccgggcg
     6241 cggcggcgaa gcgcgcggaa agctcggcct cgacttcggc cgggcgcgcg ggcaggttcc
     6301 agtccatctc ctggcgggcg acgatctgcg cgacctgcga gtacagcaac cacacccggc
     6361 agcccgcctg cagcaggcat tcgaccaggc gcacgccgta gggcaggccg gaggcgccgg
     6421 tgaaggcgac ggtgatcgtg cggggaggcg tcatcgaggt gcgggaaggt cggcggatgg
     6481 cgtgaagcgt accccatccg gcggacgagg ggcgcggctt cgatacggcc gcgggaagcg
     6541 ggcgaggggc cgcctgcacc cgcggccctt cgctgcgctc cgggctgccc tcggccttgc
     6601 cgcgtcaatg cgtcatcgtc ggctcacaca ccgcgcagct ttccagtggg agcgacgcaa
     6661 gtcgcgattg caccggcaaa gctgcgtcga cgcgaatccc gtgcggcgga atcgcgactt
     6721 gcgtcgctcc cacgaaaacc ggcacggccc cgggaagctc tgccgccgca tcccagtacc
     6781 cgtggaaggc ggcagggaaa ccaccgcggt cggggtgacg ctccggcagg agcgacgcaa
     6841 gtcgcgatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg cagctttcca
     6961 gtgggagcga cgcaagtcgc gatacgccga tcgatggagc gcggcgcgcg cctcactcca
     7021 ggtagtcggt gatcagctcc cagcgtccgt tgcggatctg ggcgaggcgg cccttgcggt
     7081 tgccgaggtg gtcgtcggtg ctgaagcggt aggcgggact gccgaagtag tcgcgggtga
     7141 acgccatgct ttcgagcgtg tgcgcgaagc gctcgggggt gagctcgcgc ccggtggcct
     7201 cggcggtgcg cacgaagagg tcggcgaggg tgtagcccat cacgctccac acgttgggct
     7261 cggccttgaa acgcgcgcgg tagcgctcga tccaggcggc gagctggctg ttcgcgccct
     7321 cggcgtaggg gtgcggcagc accgagacgc cgtacaggcc ctcgaccgcg gcgccgccga
     7381 gctcgtgggt ctgcgccgaa tagccggagg cggtgacgag catgtcgacg tcccagccga
     7441 tcttgcgcgc ttcggacatg gcggcgacgg tctcgcgcac cacggtggcg agcaccacga
     7501 ggtcgcagcg cgcggcgcgc aggcgcgcga tctggctgga gaagtcggtg gcgccgcgct
     7561 tgtagctggt gcgctcgacg agctcggtgc cgagcccggc cagcgccttt tcgacgccct
     7621 tcatgacttc caggccgtag tcgtcgtcct ggtagagcag gcagctgcgc tggtagccgc
     7681 gctcgcgcac catgtggcgg gtggcggctt ccatgtagtc ctgatagggc gcgaagttct
     7741 ggaacttgag cgggtgcagc ggctcgtagg tcgcacggtg gggcgagaag gggaacaggt
     7801 gcaggcggcc ggcatcgacg atgatcggca tgctcgccat gaccaccggc gtgcccatgt
     7861 tggcgatgaa ggcgaaggcc tgatcgtgct gcaccagctt gcgcgcggcg agcaccgcgc
     7921 gcttggggtc gtagccggcg tcctcgaccg caaggcggat cttgcgccca tgcacgccac
     7981 cgccggcgtt ggcctcgtcg aagcgcatca gcatgccgtc gcgcaccggc acgccgagca
     8041 tggcgatcgg gccggagagg tcggacacgg tgccgacgac gatttcatcg gcgctcaccc
     8101 ccgccgccac ggcgggcagg gcgcaggcga gggacagcga ggaggcgagc gcgagcgcaa
     8161 gccgacgcaa ggggctcatc gggagaactc ctggaggttg gttggattgc cgtgcgcggc
     8221 tacggcatgg cgaccgcgga gcgcgcgcgc gtggcccgca agccgggccg gtgggtgatg
     8281 cgaagcgtgg atcagaacgg caggcggcgg cgcggggctg cggcggcgcc gggcttgggc
     8341 tcgggcctgg tctccgccgg gcgcgccttg accggcttgc gcggctcgtt gagctcgtcg
     8401 agcgcgaagc gcaccttgcc ctcgagttcg cagccgcgca tgcccggcga gcagatcgcg
     8461 ccgcgcacgc gttggcagat tccatcgagg tcgtgtgggc aactccaggc actcatggcg
     8521 ggctccggcg caaactgcat gcagaattac gagagcgtta ctatcgtctg ctgcagcgcg
     8581 agaatcaagc gccgtcccac ctcggcggcc tcaccggtcg tgcctcttcg tttcgcccgc
     8641 gagtccggcc gggcgcagca gcacgacgcc gacgatcacc gcgccgaaca gcagcagctc
     8701 cagccccgag gcgcgcgccc agtcgccggg caggcggtcg cgcagcagcg agatcagcgg
     8761 cgggatggcg acgatcacca gcgcccccca ggcggcgccg gcgaggcggc gtgcgccgcc
     8821 gatgaaggcg agcatgagca actcgaagga gaacaccagg ccgaactgct ccgggctgat
     8881 gaagccgatc cagtgcgcgt agagcccgcc cgccagcccg gacagcgcgc cgccgaccac
     8941 gaaggcctgc atcttcgccg cgtcgttgtc gatgccgcag gcggcggcgg cgatttcgtc
     9001 gtcgcgcacg gcgcgccagg cgcgcccgtg gcgcgaggcg aaaaggcgtg cgcaagccag
     9061 catcgcgagc gcgagcaccg ccacgctgac cgtggcctgc gccagggtgg agtccgcgcg
     9121 ccagccggcg agggtgaagg cccccaccgt gaggcccgcg gcgccgtggg taagcgactc
     9181 ccagcgtgcg agcgcctcct cgacgatgag ggcgaaggcg agcgtgctca taccgaaata
     9241 cagcccgccc agccgccgcg ccggcaggct ggcgagcgcg ccg
//
LOCUS       DAOQNN010000090         9254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3654_length_9254_cov_7.166866, whole genome shotgun sequence.
ACCESSION   DAOQNN010000090 DAOQNN010000000
VERSION     DAOQNN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3654_length_9254_cov_7.166866"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1303)
                     /locus_tag="PKV97_06860"
     CDS             complement(98..1303)
                     /locus_tag="PKV97_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNB05633.1"
                     /translation="MNPDMLSFDEAYEKLLGFVRPVREVEEVDTMFAAGRVLAVDQHS
                     TINQPPMDNSGMDGYALRAADVPVAGTRLPVSQRIPAGSVGHELAPGTAARIFTGAPL
                     PPGADTVVMQELCEHAGDEVVVNTVPRLGEAVRRMGEDIARGARVLPAGLRLTPQAVA
                     LAASVGLARLPVHRRVRGAVFSTGSELVMPGEPLPPGGIYNSNRYLLRGLLAGLGCEV
                     EDFGIVPDRLDATREVLRRAAEGHDLILTSGGVSVGEEDHVKPAVEAEGSLDMWKIAM
                     KPGKPLAYGRVHGAAFIGLPGNPVSSFVTFLLMVRPFLLATQGVSEVAPASMQLRADF
                     DWPRPDRRREFLRARMNAQGGVELFANQGSAALESTVWANGLVDIPAGTVIACGETVR
                     FLPYGDLLH"
     gene            complement(1300..1794)
                     /gene="mobB"
                     /locus_tag="PKV97_06865"
     CDS             complement(1300..1794)
                     /gene="mobB"
                     /locus_tag="PKV97_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNB05634.1"
                     /translation="MKVFGFAGWSGSGKTTLVEKLIPEFTARGLRVSVIKHAHHGFDL
                     DKPGKDSWRHREAGAMQVLMLSNDRWVLMHELRGAAEPTLDEQLRLLEPCDLVLIEGY
                     KAAAVPKIEIHRPSHGKPPLWPENPHVVAVATNAEIDCPLPRLPLDEPAKVADFILDY
                     FKNR"
     gene            complement(1872..3833)
                     /locus_tag="PKV97_06870"
     CDS             complement(1872..3833)
                     /locus_tag="PKV97_06870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNB05635.1"
                     /translation="MSDPAPIFADDGPLAAAIPGFRARPQQIEMAQKIAEALRENRVL
                     VAEAGTGTGKTFAYLVPALLAGGKAIISTGTKTLQDQLFNRDLPTVRAALKVPVTIAL
                     LKGRANYVCHYHLERNARDGRFLTAQDAADLRAITRFARLTQTGDKAECTDVREDSLA
                     WLAATSTRDNCLGQDCPHKDECFVMQARRNAMEADVVVVNHHLFFADVMLRDEGMGEL
                     LPACNAVIFDEAHQLPETASLFFGDSVSTAQVLELARDTRSETVAAARDCVAMIDQTR
                     NLEKAARDLRLVFGPESARLSAAQAAEHENFDAMVEALEKALADFHAVLATQAERSEG
                     LGNCLRRTEEMSERLASWRKPEDKELIRWVEVFTQSLALNATPLHVSDVFKRQLEGHP
                     RAWIFTSATLAVGKADFGHYCRELGLAWMDPPPLTAVWGSPFDYAEQALLYAPAGMPE
                     PNSPDYTERVAKVALPLIRAARGRAFVLCTSLRAMRCIHELILDGLAHSGDELPVLLQ
                     GEGSRTELLERFRRLGNAVLVASQSFWEGVDVPGDALSLVVIDKLPFAPPDDPVLAAR
                     VEHMQKQGLSPFVHHQLPKTVINMKQGAGRLIRSERDRGVLCICDPRMIDKSYGKVVW
                     RSLPPMRRTRAEADAVAFLESLPPPRQGG"
     gene            4219..4755
                     /locus_tag="PKV97_06875"
     CDS             4219..4755
                     /locus_tag="PKV97_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNB05636.1"
                     /translation="MPTYSRRHTAALRSLPNTLPTLAWLLALTSCAWVAAELFWQLAA
                     PAPVAAVARHETDARKVAARIGLHVGRAAPVADKAPMRAAGPDARYTVTGIATGFGAL
                     PGFAILQAEDGSSLSLSPGQALPDGRRLVRLLPEAAEFELDGRRSTLALPARASERGE
                     SSRPPTPVADGGPRRDPR"
     gene            4752..6938
                     /gene="gspD"
                     /locus_tag="PKV97_06880"
     CDS             4752..6938
                     /gene="gspD"
                     /locus_tag="PKV97_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HNB05637.1"
                     /translation="MTRLSTLRPLPRSLPARGQAVLRNALRALAIAALGLGSCLVHAA
                     GEPVALNFQNAEIDAVIQAIGRISGRNFLIDPRVKGKINLVTNTPVAPELTYQILLSA
                     LRLQGYAAIEESGVTKLVPEADAKLHGVPVVSGRPGAGAARGDRLVTQVFALRHESAV
                     HMVNVVRPLVSANNAVTAFPGNNTLVVTDYAENLRRLATVIDSIDTPQGDVAVLQLQH
                     AMANDLAPVLVRLLAEQQTGGQNAGGLAPPQIVAEPNSNSLLVRADTPSRLAALRQLV
                     TALDRPGAGGDIRVVYLRNADAVQVAATLNAALAGEGGTSDAASTPPLSRNSGGGNGF
                     GTGSSAPSGMGVASAPLRGTGSTPGSAAGGLSSATRGGMVQPDAVNNALIITAPDAVY
                     RNLRAVIDQLDRRRAQVYIEALIAEISTERAAEFGFQWVAGASAGEVGVLGAQLFNSG
                     GGNLGQLIAGSADRTRLTLPNNGLNLVVGGGKVNVPGIGEVLSLGVLARFLENEVSAN
                     ILSTPNLITLDNEEARIVVGRNLPFVTGQYANTGGGTTPANPFQTIERRDVGLTLHVK
                     PQISEGGAIRLQIYQEASAVVGSAESAAAGNSVTTKRSIESMVLVDDGAILALGGLVE
                     DSYSGGEEKVPVLGDLPFAGSLFRYDTRKRAKTNLVVFLRPVILRDRESYAALTDSRY
                     DYVIGRRDQLPSGANLLRDPPPGEPAPVVDLAFRPLQPAAVPAAPR"
     gene            6938..8371
                     /gene="gspE"
                     /locus_tag="PKV97_06885"
     CDS             6938..8371
                     /gene="gspE"
                     /locus_tag="PKV97_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNB05638.1"
                     /translation="MGFELPYGFARDHGVLVAALGAEHAEVLVREDVRPQALAEVRRS
                     VGLPLRLQRVPRALFDARLAEAYGGAGGSAAEIVADVGQEVDLDRLMTELPAVEDLLD
                     TQDDAPIIRLINALLTQAVRDGASDVHIEPYERESVVRLRRDGVLRDIARPRRGLHAA
                     MASRIKIMAQLDIAEKRLPQDGRITLRLAGRAVDVRVSTVPTTHGERLVLRLLDKQAG
                     QIGLDGLGMAHDTSAVFTDLLARPHGILLVTGPTGSGKTTTLYAALQSLDAGRLNIVT
                     VEDPVEFDLPGVGQIPVNPRIELDFARALRAILRQDPDVIMIGEIRDLETAQIAIQAS
                     LTGHLVLATLHTNDAASAVTRLVDMGVEPFLLASTLRGVLAQRLVRKLCPDCRQPAAP
                     ASSTRGWEAGQCAHCGHSGYRGRAGIYELLVVDEGLRRLIHDGADEDRLRAHAHAAGM
                     HSLHEDGLRHVASGLTSAAELQRVIRA"
     gene            8382..>9254
                     /locus_tag="PKV97_06890"
     CDS             8382..>9254
                     /locus_tag="PKV97_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNB05639.1"
                     /translation="MSAFRYRAFDLQGTPSTGVIEADSGRAARSALRERGLHPVEVID
                     LGQQARAAADRPGWLARVQARRRLPVNDLALLSRQWATLLAAGLTVEHALSALIEQAE
                     SERVHDTLAAIRSDVVSGHSLRIALDRHSGAFPPIYRAAIAAGEQSGELAAVMNQLAD
                     HLERGADLQRKTMQALIYPALVAMVALLVVTGLMVFVVPQVVGVFAHSKQALPLLTRI
                     MIGVSNFIRDWGWLALAAGAAALLGALALLRDERIRHAFDRRLLALPVLGRHLRSLDA
                     TRFASTLAILVGSGV"
BASE COUNT         1309 a         3502 c         3142 g         1301 t
ORIGIN      
        1 cgcgcaggct cgcgaaatac agaatcttca ccttgctcgt catgtccgtc tcgttccctt
       61 gattgcccgt ccgcgtccgc tcgtccccat ctgcgcctca gtgcagcagg tcgccgtagg
      121 gcaggaagcg caccgtctcg ccgcacgcga tcaccgtgcc ggccgggatg tcgaccaggc
      181 cgttggccca cacggtggac tcgagcgccg ccgagccctg gttggcgaag agttccaccc
      241 cgccctgcgc gttcatgcgc gcgcgcagga actcacgccg gcggtcgggc cgcggccagt
      301 cgaagtcggc gcgcaactgc atcgacgccg gagcgacctc gctcactccc tgggtggcga
      361 gcaggaaggg ccgcaccatc aacaggaagg tcacgaagct cgacaccggg ttgcccggca
      421 gaccgatgaa ggcggcacca tgcacccgcc cgtaggccag cggcttgccc ggcttcatcg
      481 cgatcttcca catgtccagg ctgccctcgg cctcgaccgc cggcttgacg tggtcttcct
      541 cgcccaccga cacgccgccg ctggtgagga tgaggtcgtg gccctcggcc gcgcggcgca
      601 gcacctcgcg ggtggcgtcg aggcggtcgg gcacgatgcc gaagtcctcg acctcgcagc
      661 ctagcccggc aagcaggccg cgcagcaggt accggttgga gttgtagatg ccgcccggcg
      721 gcaggggctc gcccggcatc accagctcgg agccggtgga gaacaccgcg cccctgacgc
      781 gccgatgcac cggcagccgg gccagcccca ccgacgcggc gagcgccacc gcctgcgggg
      841 tgaggcgcaa ccccgcgggc agcacgcgcg cgccgcgggc gatgtcctcg cccatgcggc
      901 gcaccgcctc ccccagccgc ggcacggtgt tcaccaccac ctcgtcgccg gcgtgctcgc
      961 acagctcctg catcaccacg gtgtccgcac ccggcggcag cggcgcaccg gtgaagatgc
     1021 gtgcggcggt gccgggcgcg agctcgtggc cgacgctgcc ggcggggatg cgctggctca
     1081 ccggcaagcg ggtgcccgcc accggcacgt cggcagcgcg cagtgcgtag ccgtccattc
     1141 cggagttgtc catcggcggc tggttgatgg tggaatgctg gtcgaccgcg agcacgcggc
     1201 cggcggcgaa catcgtgtcc acctcctcca cctcgcgcac cgggcggacg aagccgagca
     1261 gcttctcgta ggcctcgtcg aaggaaagca tgtcggggtt catcggttct tgaagtagtc
     1321 caggatgaag tcggccacct tcgccggctc gtcgagcggc agacgcggca gcgggcagtc
     1381 gatctcggcg ttggtggcga ccgcgaccac gtgcgggttc tcgggccaca gcggcggctt
     1441 gccatgcgaa gggcggtgga tctcgatctt gggcaccgcc gccgccttgt agccttcgat
     1501 cagcaccagg tcgcagggtt cgagcaggcg cagctgctcg tccaacgtgg gctcggcggc
     1561 gccgcgcagc tcgtgcatca gcacccagcg gtcgttcgac agcatcagca cctgcatcgc
     1621 gccggcctcg cggtggcgcc acgagtcctt gcccggcttg tcgaggtcga agccgtggtg
     1681 ggcgtgcttg atcaccgaca cgcgcaggcc gcgcgcggtg aactcgggga tcagcttttc
     1741 gaccagagtg gtcttgccgg aaccggacca gccggcgaaa ccgaagactt tcatcgagac
     1801 atcgccccac ggcgtgaatt gagcagtccc gaaggataca acagagcgcc gcccgccacc
     1861 cttgacccag ctcagccgcc ctggcgcggc ggcggcaggc tctcgaggaa ggccaccgcg
     1921 tcggcctcgg cgcgcgtgcg gcgcatcggc ggcaggctgc gccacaccac cttgccgtag
     1981 gacttgtcga tcatgcgcgg gtcgcagatg cacagcacgc cgcggtcgcg ctcgctgcgg
     2041 atgagccgcc cggcgccctg cttcatgttg atcacggtct tgggcagctg gtgatgcacg
     2101 aaggggctca agccctgctt ctgcatgtgc tcgacgcgcg cggccagcac cggatcgtcg
     2161 ggcggggcga aggggagctt gtcgatcacc accagcgaca gcgcgtcgcc cggcacgtcc
     2221 acgccctccc agaagctctg gctcgccacc agcaccgcgt tgcccaggcg acggaagcgc
     2281 tcgagcagct cggtgcgcga gccctcgccc tgcagcagca cgggcagctc gtcaccgctg
     2341 tgcgcgaggc cgtcgaggat cagctcgtgg atgcaccgca tcgcgcgcag agaggtgcac
     2401 aacacgaagg cccgcccgcg cgcggcgcgg atcagcggca gcgccacctt ggcgacgcgc
     2461 tcggtgtagt cgggcgagtt gggctcgggc atgcccgcgg gcgcatacag cagggcctgc
     2521 tcggcgtagt cgaaggggct gccccacacc gcggtgagcg gcggcgggtc catccacgcc
     2581 aggccgagct cgcggcagta gtgaccgaag tcggccttgc ccaccgccag cgtcgccgag
     2641 gtgaagatcc acgcgcgcgg gtggccttcg agctggcgct tgaacacgtc cgacacgtgc
     2701 aggggggtgg cattgagcgc gagcgactgg gtgaagacct cgacccagcg gatcagctcc
     2761 ttgtcttcgg gcttgcgcca gctcgccagg cgttcggaca tctcctcggt gcggcgcagg
     2821 cagttgccca ggccctcgga gcgctcggcc tgggtggcga ggaccgcgtg gaagtcggcg
     2881 agcgccttct ccagcgcctc gaccatggcg tcgaagttct catgctcggc ggcctgcgcg
     2941 gcggagagcc gcgcgctctc cggcccgaac accaggcgca ggtcgcgtgc ggccttttcc
     3001 aggttgcggg tctggtcgat catcgccacg cagtcgcgcg ccgcggccac ggtctcggag
     3061 cgcgtatcgc gcgccagctc cagcacctgg gcggtggaca cgctgtcgcc gaagaacagg
     3121 ctcgcggtct cggggagctg gtgggcctcg tcgaagatca ccgcgttgca cgccggcagc
     3181 agctcgccca tgccctcgtc gcgcaacatc acgtcggcga agaagaggtg gtgattgacc
     3241 accaccacgt cggcctccat cgcgttgcgc cgcgcctgca tcacgaagca ctcgtccttg
     3301 tgcgggcagt cctggccgag gcagttgtcg cgtgtcgagg tcgcggcgag ccaggccagc
     3361 gagtcctcgc gcacgtcggt gcattcggcc ttgtcgccgg tctgcgtgag cctggcgaag
     3421 cgcgtgatcg cgcgcaggtc tgcggcgtcc tgcgcggtga ggaagcggcc gtcgcgcgcg
     3481 ttgcgctcga ggtggtagtg gcagacgtag ttggcgcgcc ccttcagcag cgcgatcgtc
     3541 accggcacct tcaacgcggc gcgcacggtg ggcaggtcgc ggttgaagag ctggtcctgc
     3601 agcgtcttgg tgccggtgga gatgatggcc ttgcccccgg ccagcagcgc cggcaccagg
     3661 taggcgaagg tcttgccggt gccggtgccg gcctccgcca ccagcacgcg gttctcgcgc
     3721 agcgcctcgg cgatcttctg cgccatctcg atctgctgcg gacgggcacg aaaaccgggg
     3781 atcgcggcgg cgagcgggcc gtcgtcggcg aagatcgggg cggggtcgga catgatgcgt
     3841 cgtgggcttc cggggcaagc tgtacattat cccagtttcg gtgcaactag cctattcgct
     3901 tgccgcgccc acgaggctcc acggcccgct cgggcgatcg cggcacgccc cgggtccggt
     3961 cgacggcaac accactgcaa catctccagc cgatagtcca cagcctttgc cagcgacaga
     4021 acggcgatca ccccgaatgg ccccatgagg ggacggacgc cagcccacgc atgcgaactg
     4081 gcgcctgctt tcccgaaaac gcgcattccg gtggcgtccc atgccctgcg ccgaacgaca
     4141 gacgacctgt cgctgcgatc ccgtcgcgcg cgccggacat cgcgcctgcg gtcttcacca
     4201 gtgtccccgc tcctgccgat gccgacgtat tcccgccgcc acaccgccgc cctccgctcc
     4261 ctgccgaaca ccctgcccac gctcgcctgg ctgctcgcgc tgacctcttg cgcctgggtg
     4321 gcggcggagc tgttctggca acttgcggcg cccgcgccgg tggctgcggt tgcgcgccac
     4381 gagaccgacg cccgcaaggt ggccgcacgc atcggcctgc atgtgggtcg tgccgcaccg
     4441 gtcgccgaca aggccccgat gcgcgcggca ggcccggatg cacgctacac ggtcaccggc
     4501 atcgccaccg gcttcggcgc cctgcccggc ttcgcgatcc tgcaggccga ggacggctcc
     4561 agcctcagcc tgagccccgg ccaggccctg cccgatggcc gcaggctcgt ccgcctactg
     4621 cccgaggccg ccgaattcga gctcgatggc cgccgcagca cgctcgccct gcccgcgcgc
     4681 gccagcgagc gcggcgagtc ctcccgccca cccacgcccg tcgccgatgg cgggcccaga
     4741 cgcgatcccc gatgacccgc ctttccaccc tccgaccgct tcctcgttcc ttacccgcgc
     4801 ggggccaagc cgtgttgcgc aatgcgctgc gcgcgctcgc cattgccgcg ctcggcctcg
     4861 gctcgtgcct cgtgcacgcc gcgggcgagc cggtggcgct caacttccag aacgccgaga
     4921 tcgacgcggt gatccaggcg atcggacgca tctcggggcg caactttctc atcgatccac
     4981 gcgtcaaggg caagatcaac ctcgtcacca acacccccgt ggccccggag ctgacctacc
     5041 agatcctgct gtcggcgctg cgcctgcagg gctacgcggc gatcgaggag tcgggcgtga
     5101 ccaagctcgt tcccgaggcc gacgccaagc tgcacggcgt gccggtggtc agtggccgtc
     5161 cgggcgcggg cgcagcgcgc ggcgaccgcc tggtgaccca ggtgttcgcg ctgcgccatg
     5221 aatccgcggt gcatatggtc aacgtggtgc gcccgctggt ctcggccaac aacgcggtca
     5281 ccgccttccc cggcaacaac accctggtgg tcaccgacta cgccgagaac ctgcgccggc
     5341 tggcgaccgt gatcgactcg atcgacaccc cgcagggcga cgtcgcggtg ctgcagctgc
     5401 agcacgcgat ggccaacgac ctcgccccgg tactcgtccg cctgctcgcc gagcagcaga
     5461 ccggtggcca gaacgcgggc gggctcgcac cgccgcagat cgtcgccgaa cccaacagca
     5521 acagcctgct ggtgcgtgcc gacacgccga gccgcctggc cgcgctgcgc cagctcgtca
     5581 ccgcgctcga ccgtccgggc gcgggcggcg acattcgcgt ggtgtatctg cgcaacgccg
     5641 acgccgtcca ggtcgccgcg acgctcaatg ccgcgctcgc cggcgagggc ggcacgagcg
     5701 acgctgcaag cacgccgccg ctgtcgcgca acagcggcgg cggcaatggc ttcggcacag
     5761 gcagttccgc cccgtccgga atgggcgtcg ccagcgcccc cctgcgcggc accggctcca
     5821 cccccggcag cgcagcaggc ggtctttcct ccgccacgcg cggcggcatg gtgcagccgg
     5881 acgcggtgaa caacgcgctg atcatcaccg cgcccgacgc cgtctaccgc aacctgcgcg
     5941 cggtcatcga ccagctcgac cgccgccgtg cgcaggtcta catcgaggcg ctgatcgccg
     6001 agatctccac cgagcgcgcc gccgagttcg gcttccagtg ggtcgccggc gcctcggcgg
     6061 gcgaggtcgg cgtactcggc gcgcagctct tcaacagcgg cggcggcaac ctcggccagc
     6121 tcatcgccgg cagcgccgac cggaccaggc tgacgctgcc gaacaatggc ctcaacctgg
     6181 tggtgggcgg cggcaaggtc aacgtgcccg gcatcggcga agtgctcagc ctgggggtgc
     6241 tggcccgttt cctcgagaac gaggtcagcg ccaacatcct gtccacgccc aacctcatca
     6301 cactcgacaa cgaggaggcg cgcatcgtcg tcggtcgcaa cctgcccttc gtcaccggcc
     6361 agtacgccaa caccggcggc ggcaccacgc ccgccaaccc cttccagacc atcgagcgcc
     6421 gcgatgtcgg cctcaccctg cacgtcaagc cgcagatctc cgagggcggc gcgatccgcc
     6481 tgcagatcta ccaggaggcc tcggccgtgg tgggcagcgc cgagagcgcc gccgccggca
     6541 actcggtgac cacgaagcgc tcgatcgagt ccatggtgct ggtcgacgac ggcgccatcc
     6601 tcgccctcgg cgggctggtc gaggacagct acagcggcgg cgaggagaag gtgccggtgc
     6661 tcggcgacct gcccttcgcc ggcagcctgt tccgctacga cacccgcaag cgcgccaaga
     6721 ccaacctggt ggtgttcctg cgcccggtga tcctgcgcga ccgcgaaagc tacgccgcgc
     6781 tcaccgacag ccgctacgac tacgtgatcg gccgccgcga ccagctgccc tcgggcgcca
     6841 atctgctgcg cgacccgccg ccgggcgagc ccgcgccggt ggtcgacctc gccttccgcc
     6901 cgctgcagcc cgccgcggtg ccggcggccc cgcgctgatg ggtttcgagc tgccctacgg
     6961 cttcgcgcgc gaccacggcg tgctggtcgc ggccctcggc gccgaacacg ccgaggtgct
     7021 ggtgcgcgag gacgttcgcc cgcaagcgct cgccgaagtg cgccgcagcg tcggcctgcc
     7081 cctgcgcctg cagcgcgtgc cgcgcgcgct cttcgacgcc cgcctcgccg aggcctacgg
     7141 cggagccggc ggcagtgcgg cggagatcgt cgccgacgtc ggtcaggagg tcgacctcga
     7201 tcgcctgatg accgagctgc ccgcggtcga ggacctgctc gacacccagg acgacgcgcc
     7261 gatcatccgc ctgatcaacg ccttgctcac ccaggcggta cgcgacggcg cctcggacgt
     7321 gcacatcgag ccctacgagc gcgagtcggt ggtacgcctg cgccgcgacg gcgtgctgcg
     7381 cgacatcgcg cgtccgcgcc gcggcctgca cgcggcgatg gcctcgcgca tcaagatcat
     7441 ggcgcagctc gacatcgccg agaaacgcct gccccaggac ggccgcatca ccctgcgcct
     7501 ggccgggcgg gcggtcgatg tgcgcgtgtc caccgtgccc accacccacg gcgaacgcct
     7561 ggtgctgcgc ctgctcgaca agcaggccgg gcagatcggc ctcgacggcc tcggcatggc
     7621 gcacgacacc agcgccgtgt tcaccgacct gctcgcccgg ccgcacggca tcctgctcgt
     7681 caccggcccc accggctcgg gcaagacgac cacgctgtac gcggcgctgc agagcctgga
     7741 tgccggccgc ctcaacatcg tcacggtcga ggatccggtg gaattcgatc tgcccggggt
     7801 cggccagatc ccggtcaacc cgcgcatcga gctcgacttc gcgcgcgcgc tgcgcgcgat
     7861 cctgcgccag gacccggacg tgatcatgat cggcgagatc cgcgacctcg agaccgcgca
     7921 gatcgccatc caggccagcc tcaccggcca cctcgtgctc gccaccctgc acaccaacga
     7981 cgccgcctcc gccgtcaccc gcctggtgga catgggcgtc gagcccttcc tgctcgcctc
     8041 caccctgcgc ggcgtgctcg cccagcgcct ggtgcgcaag ctctgccccg actgccgcca
     8101 gcccgccgcg cccgccagct cgacgcgggg ctgggaagcc ggccagtgcg cgcactgcgg
     8161 ccacagcggc taccgcgggc gtgccggcat ctacgagctg ctggtggtcg acgagggcct
     8221 gcgccgcctg atccacgacg gcgccgacga ggaccgcctg cgcgcccacg cgcacgccgc
     8281 cggcatgcac agcctgcacg aggacggcct gcgccacgtc gccagcgggc tgaccagcgc
     8341 cgccgagctg cagcgcgtga tccgcgcctg agccatttgt catgagcgcc ttccgctacc
     8401 gcgccttcga cctccagggc acgccgagca ccggcgtgat cgaggccgac tccggccgcg
     8461 ccgcacgcag cgcgctgcgc gagcgcggcc tgcatccggt cgaggtcatc gacctcggcc
     8521 agcaggcccg cgctgccgcc gatcgcccgg gctggctcgc acgcgtgcag gcgcgccgcc
     8581 gcctgccggt gaacgacctc gcgctgctgt cgcgccagtg ggcgaccctg ctcgccgccg
     8641 ggctcacggt cgagcacgcg ctgtcggcgc tgatcgagca ggccgagagc gagcgcgtgc
     8701 acgacaccct ggcggcgatc cgctccgacg tcgtctccgg ccacagcctg cgcatcgcgc
     8761 tcgaccgcca cagcggcgcc ttcccgccga tctaccgcgc ggcgatcgcc gccggcgagc
     8821 agtccgggga gctcgccgcg gtgatgaacc agctcgccga ccacctcgaa cgcggcgccg
     8881 acctgcagag gaagacgatg caggcgctga tctacccggc cctcgtggcg atggtggcgc
     8941 tgctggtggt gaccgggctg atggtcttcg tggtgccgca ggtggtgggc gtgttcgcgc
     9001 acagcaagca ggccctgccg ctgctcaccc gcatcatgat cggggtgtcg aacttcatcc
     9061 gcgactgggg ctggctcgcg ctcgccgcgg gtgccgccgc cctgctcggc gcgctcgcgc
     9121 tgttgcgcga cgagcgcatc cgccacgcct tcgaccgccg cctgctcgcg ctgcctgtgc
     9181 tcggccgcca cctgcgcagc ctggacgcca cccgcttcgc gagcacgctc gccatcctgg
     9241 tcggcagcgg cgtg
//
LOCUS       DAOQNN010000091         9242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3664_length_9242_cov_10.859693, whole genome shotgun sequence.
ACCESSION   DAOQNN010000091 DAOQNN010000000
VERSION     DAOQNN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3664_length_9242_cov_10.859693"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1485)
                     /locus_tag="PKV97_06895"
     CDS             complement(85..1485)
                     /locus_tag="PKV97_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05640.1"
                     /translation="MDQPRTLDYTPAGVLAGGGNPVAGLEAATATLRALYKPGSQKTE
                     VAGLHVVSSFDDALREAVERSAPVGVRALAGAAPERDHAWWVRWLAGVRDAWMAVCDG
                     YWCLSPSNSVAASEEIRLRLASSVVEALKWSACDRFGPEEVLWGRIGQLFSSSPQARD
                     SVVGGDLHSIGREYLRAVAHYSINLDQVELEMGVALGHVVDICLPFLSLDRRAADVPQ
                     YVVIPEEGTIPVRQMGKHRDGEWRFAPWAAGELLTEFSRQLARSIVPAPFGRLPLEHA
                     RAAVEYLRMQWSHTPPVRTRRRYAQEASADIARGLHACAAVIGGQPLPVPRNWRVVDF
                     SRSGLQIVANSDPTRAWPDIGELLGIHFVDGEGWQLGIVRRLRMWAAHAGLGIELLAR
                     SPTLGVIDDGRASVDGILCDLPSKGEALRVLMPANAPALAEPVFVKTAGAVHKLRSLG
                     VLRREAGYQLQVFQVL"
     gene            1686..2480
                     /locus_tag="PKV97_06900"
     CDS             1686..2480
                     /locus_tag="PKV97_06900"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNB05641.1"
                     /translation="MRQYLDLMRHVLEHGDPKTDRTGTGTLSVFGWQMRFRLQDGFPL
                     LTTKKLHTRSIIHELLWFLQGETNIRYLKENGVSIWDEWADADGELGPVYGKQWRRWE
                     TADGRSVDQIARVVESIRRTPDSRRHLVSAWNPGEVDAMALPPCHALFQFYVAGGRLS
                     CQLYQRSADIFLGVPFNIASYALLTHMVAQVCELEAGDFVWTGGDCHLYSNHLEQARE
                     QLERAPRPLPTLRLNPAVRDLFGFRFEDIALEGYDPHPHIKAPVAV"
     gene            2477..2971
                     /locus_tag="PKV97_06905"
     CDS             2477..2971
                     /locus_tag="PKV97_06905"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNB05642.1"
                     /translation="MKKPEIVLIAAVARNGTIGRDNELPWRLKADLQHFRALTLGHPI
                     LMGRKTWQSLGRPLPGRRNLVVSRTPDYRAEGAECFADPAAAIAAAGEAQTLFVIGGA
                     QLYAQLLPLADRLELTEVKADVEGDAHFPAVDPARFVEERRETHHADADNAFDFDFVR
                     YCRR"
     gene            complement(3065..5308)
                     /locus_tag="PKV97_06910"
     CDS             complement(3065..5308)
                     /locus_tag="PKV97_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HNB05643.1"
                     /translation="MRFHFPIIIIDEDFRSENTSGLGIRALAKAIEEEGMEVLGVTSY
                     GDLTSFAQQQSRGSTFILSIDDEEFSSPEASAKAIADLRAFVVEIRFRNADIPIFLHG
                     ETRTTRHIPNDVLREMHGFIHMFEDTPEFIARYVVREAKNYLDSLAPPFFRALVHYAA
                     DSSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVDELGQLLDHTGPVAA
                     SERNAARIFNCDHLYFVTNGTSTSNKMVWHTTVAPGDIVVVDRNCHKSILHSIIMTGA
                     VPVFLTPTRNHYGIIGPIPLEEFSMENIRKKIEANPFAREAKNKKPRILTITQSTYDG
                     VVYNVEAIKELLDGEIDTLHFDEAWLPHAAFHDFYGDYHAIGADRPRCRESMVFATQS
                     THKLLAGLSQASQILVQDSQTRRLDRDIFNEAYLMHTSTSPQYAIIASCDVAAAMMEA
                     PGGTALVNESLSEAVEFRRAMRKVDAEFGDDEWWFQVWGPEYLAEEGIGERDDWTLKA
                     GERWHGFGNLAEGFNILDPIKATIITPGLNMDGDFAEHTGIPAAIVTKYLAEHGIIVE
                     KTGLYSFFIMFTIGITKGRWNTMVTELQQFKDDYDRNQPLWRVMPEFIAKHPRYERVG
                     LKDLCDQIHSFYKANDVARLTTEMYLSDMVPAMKPADAFAKMAHREIERVALDELEGR
                     VTAMLVTPYPPGIPLLIPGERFNATIVRYLKFARDFNARFPGFETDIHGLVKGEDGRY
                     HVDCVKG"
     gene            complement(5458..6024)
                     /gene="dcd"
                     /locus_tag="PKV97_06915"
     CDS             complement(5458..6024)
                     /gene="dcd"
                     /locus_tag="PKV97_06915"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNB05644.1"
                     /translation="MSIKSDKWIRRMAEQARMIEPFAPELVRSNDTGRIVSYGTSSYG
                     YDVRVANEFKIFTNINSTIVDPKDFDARNFVDFTGDVCIIPPNSFALARTVEYFRIPR
                     SVLTVCLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYANEGVAQMLFF
                     ESDEVCETSYRDRGGKYLGQTGVTLPKI"
     gene            complement(6216..7307)
                     /gene="apbC"
                     /locus_tag="PKV97_06920"
     CDS             complement(6216..7307)
                     /gene="apbC"
                     /locus_tag="PKV97_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNB05645.1"
                     /translation="MSLDQQTVTEALKQVIDPNTGKDFVAGRSIRNLSVQGGEVRFDV
                     ELGYPAKSQHDPIRALLSEAVAKLPGVERVAVTVSSKVVAHAVQQGVKLLPGVRNIIA
                     VASGKGGVGKSTTAVNLALALSAEGARVGLLDADIYGPSQPQMLGIGDQRPVSEDGKT
                     MTPLQAFGLQAMSIGFLIDPDTPMVWRGPMATQALNQMLKDTAWDDLDYLVIDMPPGT
                     GDIQLTLSQSVPVTGAVIVTTPQDIALLDARKGVKMFEKVGVPILGVVENMSIHICSK
                     CGHEEHIFGQGGGEKMCADFKVPFLGALPLDIQIRTEADSGAPTVVADPDGRIASIYK
                     QIARKVAVRIAERSKDMTHKFPNIVFKSA"
     gene            7470..8072
                     /locus_tag="PKV97_06925"
     CDS             7470..8072
                     /locus_tag="PKV97_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB05646.1"
                     /translation="MHMTLRNSRRRPGFPTALVSATLLAGLLAACASPGGNGTATDAI
                     HRHALVRTAPPEPSWAALREQLAKATTGLAGVEIGSAGDTGFRVQIPVADGFASGSPA
                     IRPTLGRALDALAPALAAEAGVAVRVVGHTDSQGSEMVNLRLSIARAEAVVAWLRERG
                     IALDRLAADGRGEADPLTSNATEEGRVRNRRIELILSRMP"
     gene            8150..>9242
                     /gene="metG"
                     /locus_tag="PKV97_06930"
     CDS             8150..>9242
                     /gene="metG"
                     /locus_tag="PKV97_06930"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNB05647.1"
                     /translation="MSRKLLVTNALPYANGDIHLGHLVGYIQADIWVRYQRMRGHSVH
                     YVCADDTHGTPVMLRAEKEGLTPEQLIERVHGEHLRDFTAFGVAFDNYHSTHSAENRF
                     YAEDIYARLKAGGLIDTRSIEQYYDPVKEMFLPDRFIKGECPKCGAPDQYGDNCEACG
                     AAYAPTELKNPYSAVSGAKPVLKTSEHYFFRLSDERAVAFLREWTRGTNAAGERRLQM
                     EAANKMKEWLGENATEEGENTLSDWDISRDAPYFGFEIPGAPGKYFYVWLDAPIGYLA
                     SFRNLAAKRAAAGETIAVEDYTDAARAAAAGTEMVHFIGKDILYFHALFWPAMLKFAG
                     YATPSRLCVNGFLTVDGAKMSKSRGTFITA"
BASE COUNT         1556 a         3187 c         3007 g         1492 t
ORIGIN      
        1 gacgccaacc aaagaaatcc ccgcccccct tgcatccgac aaccgcgaac ggccggtcgg
       61 atcggccaag gagcgcctaa ggacttacag aacctggaac acctgcagtt gataaccggc
      121 ttcgcgccgg agaacaccga gcgagcgtag tttatggacc gccccggcgg tcttgacgaa
      181 gaccggctcg gcgagcgcag gcgcattggc gggcatcaag acgcgcaagg cctcgccctt
      241 gctcgggaga tcgcacagga tgccgtccac gctggcgcga ccgtcatcga tcacgccgag
      301 cgtcggactg cgagccagca actcgatgcc cagacccgcg tgcgcagccc acatgcgcaa
      361 ccggcgcacg atgccaagct gccagccttc gccgtccacg aaatggatgc cgaggagttc
      421 accgatgtct ggccacgcgc gcgtcgggtc gctgttggcg acaatctgca ggccgctgcg
      481 actgaaatcg accaccctcc agtttcgcgg caccggcaag ggctgcccgc cgatcaccgc
      541 ggcacaggcg tgcaatcccc gcgcgatatc tgcgctcgcc tcctgggcgt aacggcgtcg
      601 tgtgcgcacc ggaggggtat gcgaccactg catgcgcagg tattcgacgg ctgcgcgggc
      661 atgctcgagt ggcaggcgcc cgaacggagc gggcacgatc gagcgcgcga gctgcctcga
      721 gaactcggtc agcaactccc ccgccgccca cggggcgaaa cgccactcgc catcccggtg
      781 cttgcccatc tggcgcaccg ggatggttcc ctcctcgggg atgaccacat actgcggaac
      841 atccgccgcg cgccgatcga gggagagaaa cggaaggcaa atatccacca cgtgcccgag
      901 cgcgacgccc atctcgagct cgacctgatc gaggttgatg ctgtagtggg cgaccgcgcg
      961 caggtattcg cgcccgatgc tgtgcaagtc tccgccgacg acgctgtcgc gggcctgcgg
     1021 cgaggaagag aacaactggc cgatgcgccc ccagagcacc tcctcggggc cgaagcgatc
     1081 gcaggcggac cacttcagcg cctcgaccac gctgctcgcg agacgcaacc ggatctcttc
     1141 ggaggcggcc accgagttcg acggggagag acaccaatag ccatcgcaca ctgccatcca
     1201 ggcatcccgg acccctgcga gccaacgcac ccaccacgcg tggtcacgct cgggcgcggc
     1261 gcctgccaga gcgcgcaccc cgaccggcgc cgagcgctcg actgcttcac gcaaggcgtc
     1321 gtcgaagctg gacaccacat gcaagcccgc gacctccgtc ttctggcttc ccggtttgta
     1381 caatgcccgc agcgtcgcag tcgccgcctc caggcccgcg accgggttgc ctccaccagc
     1441 gagcacaccg gcaggcgtgt agtcgagcgt cctgggctgg tccaaagggc tttctccgca
     1501 caaaccgcgc cctgcctgtg ttccccaggg aaaacaggcg tcgcacgaaa gatttcgtca
     1561 cattctgaac cagcgtccac ggcgatgtcc accgcataca cgccgggacg aagcgatcgt
     1621 atcgccaccg ccatcacaga tccttcgccc tgcacagtag aatctcgcct tttccgggag
     1681 cgacgatgcg acagtacctc gacctgatgc gccatgtgct cgagcacggc gaccccaaga
     1741 ccgaccgcac cggcaccgga accctgtcgg tgttcggctg gcagatgcgc ttccgcctgc
     1801 aggacggttt tccgctcctg accaccaaga agctgcacac gcgctcgatc atccacgagt
     1861 tgctgtggtt cctgcagggc gagaccaaca tccgctacct caaggaaaac ggcgtgtcga
     1921 tctgggacga atgggcggac gccgacggcg agcttggccc ggtgtacggc aagcagtggc
     1981 ggcgctggga gaccgcagac gggcgcagcg tcgaccagat cgcacgcgtc gtcgaaagca
     2041 tccggcgcac cccggattcg cgccgccatc tcgtctcggc atggaatccg ggcgaagtcg
     2101 acgccatggc cctgccgccc tgccacgcgc tcttccagtt ctacgtcgcc ggcggtcgcc
     2161 tgtcgtgcca gctctaccag cgcagcgcgg acatcttcct cggcgtgccc ttcaacatcg
     2221 cctcctatgc cctgctcacc cacatggtgg cgcaggtgtg cgagctcgaa gcgggcgatt
     2281 tcgtgtggac cggtggcgac tgccacctct acagcaacca cctggagcag gcgcgcgagc
     2341 agctcgagcg cgccccgcgc cccctgccga cgctgcgcct gaaccccgca gtgcgcgacc
     2401 tcttcggctt ccgcttcgag gacatcgcgc tcgaaggcta cgacccgcat ccgcacatca
     2461 aggccccggt ggcggtatga agaagcccga gatcgtcctc atcgccgcgg tcgcgcgcaa
     2521 cggcacgatc ggccgcgata acgagctgcc ctggcggctg aaggccgacc tgcagcattt
     2581 ccgtgccctc accctggggc acccgatcct gatggggcgc aagacctggc aatcgctcgg
     2641 ccgcccgctg ccaggacggc gcaacctcgt ggtgagccgt acgccggatt accgtgccga
     2701 aggcgccgaa tgcttcgccg accccgcggc cgcgatcgcc gcggccggcg aggcccagac
     2761 actgttcgtg atcggcggcg cccaacttta cgcccagctg cttcccctcg ccgaccgcct
     2821 cgagctcacc gaggtgaaag ccgacgtcga gggcgatgcg cacttccccg ccgtcgatcc
     2881 ggcgcggttc gtcgaggagc gtcgcgagac acaccacgcc gacgccgaca acgccttcga
     2941 cttcgatttc gtgcgctact gccggcgctg agcccgacgg ccgcgccgac tgccctgccg
     3001 cagaaacgac aagggccgca tcgcgcgacc ctcgtcattc gacaccccct gcggcgggag
     3061 cggctcagcc cttcacgcaa tccacgtggt agcgcccgtc ctcgcccttg accaggccgt
     3121 ggatgtcggt ctcgaagccg gggaagcggg cgttgaagtc gcgcgcgaac ttgaggtagc
     3181 gcacgatcgt cgcgttgaag cgctcgcccg ggatcagcag cgggatgccc ggcggatagg
     3241 gcgtgaccag catcgcggtg acgcggccct cgagctcgtc gagcgccacg cgctcgatct
     3301 cgcggtgcgc catcttggcg aaggcgtcgg cgggcttcat cgccggcacc atgtccgaca
     3361 gatacatctc ggtggtcagt cgggcgacgt cgttggcctt gtagaagctg tggatctggt
     3421 cgcacagatc cttgagcccg acgcgctcgt agcgcggatg cttggcgatg aattcgggca
     3481 tcacgcgcca cagcggctgg ttgcggtcgt agtcgtcctt gaactgctgc agctcggtca
     3541 ccatcgtatt ccagcggccc ttggtgatgc cgatggtgaa catgatgaag aaggagtaca
     3601 ggccggtctt ctcgacgatg atgccgtgct ccgcgaggta cttggtgacg atcgccgccg
     3661 ggatgccggt gtgttcggcg aagtcgccgt ccatgttcag gcccggggtg atgatcgttg
     3721 ccttgatcgg gtcgaggatg ttgaagccct cggccaggtt gccgaagcca tgccagcgct
     3781 cgccggcctt cagcgtccag tcgtcgcgct cgccgatgcc ctcctcggcc aggtactccg
     3841 gcccccagac ctggaaccac cattcgtcgt cgccgaattc ggcgtccacc ttgcgcatcg
     3901 cgcgacggaa ctccaccgcc tcggagagcg attcattgac cagcgcggtg ccgcccggcg
     3961 cctccatcat cgccgccgcc acgtcacagg acgcgatgat cgcgtactgc ggcgaggtcg
     4021 aggtatgcat gaggtaggcc tcgttgaaga tgtcgcggtc gagcctgcgg gtctgcgagt
     4081 cctgcaccag gatctgcgag gcctggctga gtccggcgag cagcttgtgg gtggactggg
     4141 tggcgaacac catcgactcg cggcagcgcg gacgatcggc gccgatcgcg tgatagtcgc
     4201 cgtagaagtc gtggaaggcg gcgtgcggca gccaggcttc atcgaagtgc agggtgtcga
     4261 tctcgccgtc gagcagctcc ttgatggcct cgacgttgta caccacgccg tcgtaggtcg
     4321 actgggtgat ggtgaggatg cgcggcttct tgttcttcgc ctcgcgcgcg aaggggttgg
     4381 cctcgatctt cttgcggatg ttctccattg agaactcctc cagcgggatc gggccgatga
     4441 tgccgtagtg gttgcgcgtc ggcgtcagga acaccggcac cgcgccggtc atgatgatcg
     4501 agtggaggat ggacttgtgg cagttgcggt cgacgaccac gatgtcgccg ggcgccaccg
     4561 tggtgtgcca gaccatcttg ttcgacgtcg acgtgccgtt ggtgacgaag tacaggtggt
     4621 cgcagttgaa gatgcgcgcc gcgttgcgct ccgaggccgc caccggcccg gtgtggtcga
     4681 gcagctggcc gagttcgtcg accgcattgc acacgtccgc gcgcagcatg ttctcgccga
     4741 agaactggtg gaacatctgc cccaccgggc ttttcagaaa agcaacgcca cccgagtgcc
     4801 ccgggcagtg ccacgagtac gagctgtcgg ccgcgtagtg caccagcgcg cggaagaagg
     4861 gcggcgccag cgaatcgaga tagttcttcg cctcgcgcac cacgtagcgc gcgatgaact
     4921 ccggcgtgtc ctcgaacatg tggatgaagc cgtgcatctc gcgcagcacg tcgttgggga
     4981 tgtggcgcgt ggtgcgcgtc tcgccgtgca ggaagatcgg gatgtcggcg ttgcggaagc
     5041 ggatctcgac gacaaacgcg cgcaggtcgg cgatcgcctt ggccgaggcc tcgggcgagg
     5101 agaactcctc gtcgtcgatc gacaggatga aggtcgagcc acggctctgc tgctgcgcga
     5161 aggacgtcag gtcgccgtag ctggtgacgc cgagcacttc catgccctct tcctcgatcg
     5221 ccttggcgag cgcgcggatc cccagtcccg aggtgttctc cgagcggaag tcctcgtcga
     5281 tgatgatgat ggggaagtgg aatctcatcc tggtcgtctc cagcgggtcc gtgcgggccg
     5341 ggtaaagccg gcagtgtact ccggcgaaga ttgcccgcgc gttaccgaaa ttggttcgaa
     5401 aaagcacaag gggccgtcag gcggccccgt ggaggaaggt cggcgaagcg aaggcgctca
     5461 gatcttgggc agggtgacgc cggtctgccc gaggtacttg ccgccgcgat ccctgtacga
     5521 ggtctcgcac acctcgtccg actcgaagaa cagcatctgc gccacgccct cgttggcgta
     5581 gatcttcgcc ggcagcggcg tggtgttgga gaactcgagg gtcacgtggc cttcccactc
     5641 gggctcgagc ggggtgacgt tcacgatgat gccgcagcgc gcgtaggtgc ttttgcccag
     5701 gcacacggtc agcacgctgc gcggaatgcg gaagtactcg accgtgcgcg cgagtgcaaa
     5761 ggagttcggc gggatgatgc acacgtcgcc ggtgaagtcg acgaagttgc gcgcatcgaa
     5821 gtccttgggg tcgacgatgg tcgagttgat gttggtgaaa atcttgaatt cgttcgccac
     5881 gcgcacgtcg tagccatagc tcgaggtgcc gtaggacacg atgcggccgg tgtcgttgct
     5941 gcgcaccagt tccggcgcga agggctcgat catgcgggcc tgttcggcca tgcggcggat
     6001 ccacttgtcg gacttgatgg acatgggcgc gtcttccggc gggttgcggt gcgggcggcg
     6061 gaggccgtcc gtgaaaagcc gcgtagtgta aggcaggcaa gcggcggacg aaaggcggag
     6121 ggaaaacgaa tcgcggccgg atcgcgcgaa gttggcgcac ggcgggcacg ctcgacgtgc
     6181 gcgcccgccg ctacggacgg gcgcggcctg cgcaatcagg cggacttgaa cacgatgttg
     6241 gggaacttgt gcgtcatgtc cttcgagcgc tcggcgatgc gcaccgccac cttgcgcgcg
     6301 atctgcttgt agatcgaggc gatgcggcca tccggatcgg cgaccacggt gggcgcgccg
     6361 ctgtcggcct cggtgcggat ctggatgtcg agcggcagcg caccgaggaa gggcaccttg
     6421 aagtccgcgc acatcttctc gccgccaccc tggccgaaga tgtgctcctc gtggccgcac
     6481 ttcgagcaga tgtggatgct catgttctcg accacgccga ggatgggcac gcccaccttc
     6541 tcgaacatct tcacgccctt gcgcgcgtca agcagcgcga tgtcctgcgg cgtggtgacg
     6601 atcaccgcgc cggtcaccgg cacgctctgc gacagggtga gctggatgtc gccggtgccc
     6661 gggggcatgt cgatgacgag gtagtcgagg tcgtcccagg cggtgtcctt gagcatctgg
     6721 ttgagcgcct gcgtggccat cgggccgcgc cacaccatcg gcgtatcggg gtcgatcagg
     6781 aagccgatcg acatcgcctg cagcccgaag gcctgcagcg gggtcatggt cttgccgtcc
     6841 tccgacaccg ggcgctggtc gccgatgccc agcatctgtg gctgcgaggg accgtagatg
     6901 tcggcgtcga gcaggccgac gcgggcacct tcggccgaca acgcgagcgc gaggttcacc
     6961 gcggtcgtgc tcttgcccac gccgcccttg cccgaggcca ccgcgatgat gttgcgcacg
     7021 cccggcagca gcttaacgcc ctgctgcacc gcgtgcgcga ccaccttgct cgacaccgtc
     7081 acggcgacgc gctccacgcc cggcagcttg gccacggcct cgctcagcag cgcgcggatc
     7141 ggatcgtgct ggctcttcgc cgggtagccg agctcgacgt cgaagcggac ctcgccaccc
     7201 tgcaccgaca ggttgcggat gctgcgcccc gccacgaaat ccttgccggt gttgggatcg
     7261 atcacctgct tgagcgcttc ggttacggtt tgctgatcaa gactcatcgc gttttcctcg
     7321 aaaatcggtg cacccgccgc cgggtggaag cggcagaatc ataccggagc gcagcgccgg
     7381 actcatccgc gttctgcgca gggtttctcg cccgcccccg cacccgaccc cgttcgacga
     7441 atttcccgcg cgccgcatgc aggcgccaca tgcacatgac tttgcgcaat tcccgccgca
     7501 gacccggctt cccgacggcc ctcgtgtccg ccaccctgct cgccggcctc ctcgccgcct
     7561 gcgccagccc tggcggcaat ggcacggcca ccgacgcgat ccaccgccat gcgctggtgc
     7621 ggaccgcacc gcccgaaccg tcctgggccg cacttcgcga gcagctcgcc aaggccacca
     7681 ccggcctcgc gggagtcgag atcggcagcg ccggcgacac gggcttccgc gtgcagatcc
     7741 cggtcgccga cggcttcgcc agcggcagtc cggcaatccg tccgacgctc ggacgcgcgc
     7801 tcgacgcgct cgcgcccgcg ctcgcggccg aggccggagt ggcggtgcgg gtggtcggcc
     7861 acaccgacag ccaaggcagc gagatggtca acctgcgcct gtcgatcgcg cgcgccgagg
     7921 cagtggtggc gtggctgcgc gagcgcggca tcgccctcga ccgcctcgcc gccgacggtc
     7981 gcggcgaggc cgatccgctc acgtccaacg ccaccgagga aggccgcgtg cgcaaccgcc
     8041 gcatcgagct gatcctctcg cgcatgccct gagcgcgcgc cacggcgcgg catcgcgtgc
     8101 cgctttgcta aactctcgac tttatccggc accggttccc gcacccatca tgtcccgcaa
     8161 gctcctcgtc accaacgccc tgccctacgc caacggcgac atccacctcg gccacctcgt
     8221 gggctacatc caggccgaca tctgggtgcg ctaccagcgc atgcgcggcc actcggtgca
     8281 ctacgtgtgc gcggacgaca cccacggcac gccggtcatg ctgcgcgccg aaaaggaagg
     8341 gctcacgccc gagcagctca tcgagcgcgt gcacggcgag cacctgcgcg acttcaccgc
     8401 cttcggcgtc gcattcgaca attaccactc gacgcacagc gccgagaacc gtttctatgc
     8461 cgaggacatc tacgccagac tgaaggcggg cggcctgatc gacacacgct cgatcgagca
     8521 gtactacgac ccggtcaagg agatgttcct ccccgaccgc ttcatcaagg gcgagtgccc
     8581 gaagtgcggc gcccccgacc agtacggcga caactgcgag gcctgcggcg cggcctacgc
     8641 ccccaccgag ctcaagaacc cctattccgc ggtgtccggc gccaagccgg tgctgaagac
     8701 ctcggagcac tacttcttcc gtctgtcgga cgaacgcgcg gtcgccttcc tgcgcgaatg
     8761 gacgcgcgga accaacgccg ccggcgagcg ccgcctgcag atggaagccg ccaacaagat
     8821 gaaggagtgg ctcggagaaa acgcaactga ggaaggcgag aacacgctct ccgactggga
     8881 catctcgcgc gacgcaccct acttcggctt cgagattccg ggtgcgccgg gcaagtactt
     8941 ctatgtgtgg ctggacgcac cgatcggcta cctcgccagc ttccgcaacc tcgctgccaa
     9001 gcgcgccgcc gcgggcgaga cgatcgccgt cgaggactac accgacgccg cccgcgccgc
     9061 cgcggccggc accgagatgg tgcacttcat cggcaaggac atcctctact tccacgccct
     9121 gttctggccc gcgatgctga agttcgccgg ctacgccacg cccagccggc tgtgcgtgaa
     9181 cggcttcctc accgtcgacg gcgccaagat gagcaagagc cgcggcacct tcatcaccgc
     9241 gc
//
LOCUS       DAOQNN010000092         9205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3695_length_9205_cov_9.227978, whole genome shotgun sequence.
ACCESSION   DAOQNN010000092 DAOQNN010000000
VERSION     DAOQNN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3695_length_9205_cov_9.227978"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..672)
                     /locus_tag="PKV97_06935"
     CDS             complement(154..672)
                     /locus_tag="PKV97_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HNB05648.1"
                     /translation="MLKSHAVLESRRPSLSASVAAAVLALGVALPGVAVAETVGHVDT
                     AFKLIGRNHQVVVEAFDDPSVKGVACYVSRARTGGLKGTFGLAEDTSDASVACRQVGD
                     ISFSGPLREQEEVFSQSASILFKKVRIVRMVDPKRNTLVYLVYSDKLIEGSPQNSVTA
                     VPVPAALAIPLR"
     gene            complement(825..2273)
                     /locus_tag="PKV97_06940"
     CDS             complement(825..2273)
                     /locus_tag="PKV97_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNB05649.1"
                     /translation="MAKVKFFGEESIPLEMHKVRIVQKLNLPPVERRLEAITEAGNNT
                     FLLRNADVFMDMLTDSGVNAMSDQQLAAMMIADDSYAGSATYTRLENKLHEIFGMPFF
                     LPAHQGRACENILAQVLVSPGSVVPMNYHFTTTKAHITLNGGKVEELVADVGLEVVSV
                     HPFKGNMDVAKLRDVIGIHGADKIAFVRMEAGTNLIGGQPFSVQNLADIRRVCDEFGL
                     MLVMDASLLADNLHFIKEREEACKDLSIREITRRMADLCDVIYFSARKLGCARGGGMC
                     IRSEELYRRMRGLVPLYEGFLTYGGMSVREMEALTVGLEETMDEEVINQGPQFIGFMV
                     DELVKRGIPVITPAGGLGCHINAMQFVDHLPQAQYPAGALASALYLAGGIRGMERGTL
                     SEQREPDGSELFANMELVRLAMPRRVFTLSQVKYAVDRLEWLYANRRLIGGLVFTEEP
                     EILRFFYGRLAPVSDWQNALVAQFRKDFGDSL"
     gene            2594..3121
                     /locus_tag="PKV97_06945"
     CDS             2594..3121
                     /locus_tag="PKV97_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HNB05650.1"
                     /translation="MWVGIFCEITEFALLFAVFFIARAHHPEAFHAGPARLSLLAGTG
                     YTLMLLTSGWCVARALHAMRRGDRRATVRWMLAGFVFGLGYPAIKIFELQRNFALGLD
                     GSAGVFIVSYYYLTFTHLVHVFWGLLGMMWVIARTGLGAYTPEEHSGLEAFACYWHVT
                     DMIWLMIFPLFYLLG"
     gene            3132..3425
                     /locus_tag="PKV97_06950"
     CDS             3132..3425
                     /locus_tag="PKV97_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNB05651.1"
                     /translation="MSPERKIDLAWLMLVLLSVGGAGIARSDANAVLVAVGVSLVMAI
                     KLRLVCNYFLELHEAHPRIRRAVLAFCHGMPILVILTTAFGDTLSSLTGALLG"
     gene            complement(3474..3674)
                     /locus_tag="PKV97_06955"
     CDS             complement(3474..3674)
                     /locus_tag="PKV97_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNB05652.1"
                     /translation="MEERIVVLETKLMAAEDLLDELNRTVWRQQQELDLLRQHLHQLA
                     QQVKTLQPGDPLCPEDEIPPHW"
     gene            complement(3711..4811)
                     /gene="queG"
                     /locus_tag="PKV97_06960"
     CDS             complement(3711..4811)
                     /gene="queG"
                     /locus_tag="PKV97_06960"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNB05653.1"
                     /translation="METKLPTPDGAAVDGTVLDGAALVARIRQWGRELGFDAVGVGGV
                     DLADAEPGLVAWLEAGFHGDMDYMVRHGMKRARAAELLPGSVRVISVRMGYWPDAAPA
                     MDVLGDPERAYVSRYALGRDYHKLVRNRLQKLADRISAEVPHQYRVFTDSAPILEVEH
                     ASRKGLGWRGKHTLLLDRTAGSWFFLGEILTDLPLPVDAPVASHCGRCTACIDACPTG
                     AIVAPYRLDARRCISYLTIELHGAIPEELRPLLGNRIYGCDDCQLVCPWNRFAQLGRE
                     PDFAPRQGLDDARLAELFAWTAEEFSERTAGSPIHRIGHARWLRNIAVALGNGPATPA
                     ARAALQARADDEDAVVREHVAWALARLAAAAG"
     gene            4827..5357
                     /gene="tsaE"
                     /locus_tag="PKV97_06965"
     CDS             4827..5357
                     /gene="tsaE"
                     /locus_tag="PKV97_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNB05654.1"
                     /translation="MIESIQTADDNGARLQAHLPDEAATVALGGALAGVLAPGLQIWL
                     QGNLGTGKTTLTRGLLRALGHEGKVKSPTYTLIEPYVVSSLNLYHFDFYRFTSPDEYL
                     DAGLDEYFAGEGVCIVEWPDKASPHLPSPDVEIVLQAGESGRDVAISANTEAGRTCVI
                     GLVKALRGADPAPGTT"
     gene            5297..6694
                     /locus_tag="PKV97_06970"
     CDS             5297..6694
                     /locus_tag="PKV97_06970"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNB05655.1"
                     /translation="MRDRTGESFAGRGPGARDDLSLGQTPGLAAAAEAFAAGRIDRRG
                     LLKFAGASLAMLVSPVGLASSASLLAVRVWPSAEYTRITLEGSSRLRHSHMLVEDPQR
                     LVVDLEGVQLDSVLQSLPSKVLDSDPYIRLIRAGQNRPGVVRVVIELKAAINPQVFTL
                     DPVGSYGHRLVLDLHPTEAHDPLMALIMKDSPMDAAMGDAGSNTTAVAREEPREPVRR
                     GRRNEPAVDRLYTVVLDAGHGGEDPGAIGRGGSYEKDVTLSIAQRLKRKIDAMPGMRA
                     VLTRDGDYFVPLHQRVARARRVRADLFVSIHADAFVRPEANGSSVYVLSERGASSSAA
                     RWLAQKENDADLVGGVNLARQDGHIARTLLDLSQTATINDSFKLGRAMLGELGTINRL
                     HKPEVEQAGFAVLRAPDIPSVLVETAFISNPQEERRLNDEAYQDKMAMALMRGVKRYF
                     EEHAPSGPTRVARLD"
     gene            6788..7726
                     /locus_tag="PKV97_06975"
     CDS             6788..7726
                     /locus_tag="PKV97_06975"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNB05656.1"
                     /translation="MQVHRGIPEQADTAAVLTIGNFDGVHLGHQALLSLLTDKARALG
                     LPAVVLTFEPHPREYFSPADAPARLASLREKLLLLSAAGVDRCHVCRFDARFAAQPAQ
                     AFIDDILVRGLGVRHLFIGDDFRFGARRQGNFAMLRAAGAEGGFGVESMPTLAVAGER
                     VSSSAVRAALAEGDLAHAARLLGRPYSIAGRVSHGDKLGRQLGFPTANIQMKHRRPAL
                     GGVYVVSVEGLASGPVAGVANIGLRPTATANGRARLEVHLFDWTQDCYGAHIRVHFLH
                     RLRAEQKFPSLDALRAQIALDSQTARDWLAANPLPA"
     gene            7771..>9205
                     /locus_tag="PKV97_06980"
     CDS             7771..>9205
                     /locus_tag="PKV97_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB05657.1"
                     /translation="MADYRKTLNLPDTPFPMRGDLPRREPNWITDWQQKKLYQRIRKA
                     SAGRPKFVLHDGPPYANGSLHIGHALNKILKDIIVRSKTLAGFDAPYVPGWDCHGLPI
                     EHKVEVTHGKNLPADRVRELCRAYAAEQIEIQKADFIRLGVLGDWDNPYRTMDFANEA
                     NEIRALAEMTKNGYVFKGLKPVNWCFDCGSALAEAEVEYADKPSPTIDVAFPVSDADA
                     GTHLRKLAAAFGLARLDKPAAAVIWTTTPWTIPANQALNAHPEFDYALVDVGDRLLVL
                     ARELVESALQRFQLAGTIIATAKGAALERIEFRHPFYDRVSPVYLAEYVGIDAGTGIV
                     HSSPAHGVDDFNSWHAYGRSNDEILSLVTGGGQYVSDLPFFGGMNIWKANPAIVDKLA
                     EVGALLSHGKITHSYMHCWRHKTPLVYRATAQWFVGMDRVAADGSTLRERALRAVEAT
                     KFYPAWGQARLHAMIANRPDWCISRQRN"
BASE COUNT         1418 a         3306 c         3049 g         1432 t
ORIGIN      
        1 aacccactcg cgccctcctc ggcgggcgtg accgaaaagt tcacaagctc tgaggcgccg
       61 acatgcgcac ccgcgcctcc gcatgggttc gcgccttcgc cgcagccgca agccggcgcg
      121 gcgcaccgcg cgcaggcctc gcgtgcgggg cactcaacgc agcgggatgg cgagcgcagc
      181 cgggaccggc acggcggtga cgctgttctg cgggctgccc tcgatcagct tgtcgctgta
      241 gacgagatag accagcgtgt tgcgcttcgg gtccaccatg cgcacgatgc gcactttctt
      301 gaacaggatc gaggcgctct gcgagaacac ctcctcctgc tcgcgcagcg gaccggaaaa
      361 ggagatgtcg ccgacctggc gacaggcgac cgaggcgtcg gacgtgtcct cggcgaggcc
      421 gaaggttccc ttgagaccgc cggtgcgcgc gcgcgagacg tagcaggcca cgcccttgac
      481 ggagggatcg tcgaaagcct cgaccacgac ctggtggttg cggccgatca gcttgaacgc
      541 cgtgtcgacg tggcccaccg tctcggccac ggccactccg ggcagggcga cacccagcgc
      601 gagcactgcg gctgcgaccg aggcggacag cgacggacga cgcgattcaa gtaccgcatg
      661 ggacttcagc atgaaggcct ccagaaggct gagagaccga cattatcgca cccccaagac
      721 accaccggga tgcgcgcgtc cgcatccggg cggcatgccg cggcatcgtg ccgttggcgg
      781 tcggcgcgcg gcggctccgc gcctgacggc gccgccggac ggcgtcagag gctgtcgccg
      841 aagtccttgc ggaactgcgc gacgagggcg ttctgccagt cggacaccgg cgcgaggcgg
      901 ccgtagaaga agcgcaggat ctccggctcc tcggtgaaca ccaggccgcc gatcaggcgc
      961 cggttggcgt acagccactc gagacggtcc accgcgtact tgacctgcga cagcgtgaac
     1021 acccgccgcg gcatcgccag gcgcaccagc tccatgttgg cgaagagctc gctgccgtcg
     1081 ggctcgcgct gctcggagag cgtgccgcgc tccatgccgc ggatgccgcc ggcgaggtag
     1141 agcgccgagg cgagcgcgcc cgccgggtac tgcgcctgcg ggaggtggtc tacgaactgc
     1201 atcgcgttga tgtggcagcc caggccgccg gccggggtga tcaccgggat gccgcgcttg
     1261 acgagctcgt ccaccatgaa gccgatgaac tgcggaccct ggttgatgac ctcctcgtcc
     1321 atggtctcct ccaggcccac ggtgagcgcc tccatctcgc gcaccgacat gccgccgtag
     1381 gtcaggaagc cctcgtacag cggcaccagg ccgcgcatgc ggcggtacag ctcctcgctg
     1441 cggatgcaca tgccgccgcc acgggcgcag ccgagcttgc gcgcggagaa gtagatcacg
     1501 tcgcacaggt cggccatgcg gcgggtgatc tcgcggatgc tcagatcctt gcaggcctcc
     1561 tcgcgctcct tgatgaagtg caggttgtcg gcgagcaggc tggcgtccat caccagcatc
     1621 agcccgaact cgtcgcacac cctgcggatg tcggcgaggt tctgcacgga gaagggctgg
     1681 ccgccgatca ggttggtgcc ggcctccatg cgcacgaagg cgatcttgtc ggcgccgtgg
     1741 attccgatca cgtcgcgcag cttggcgacg tccatgttgc ccttgaaggg atggacgctg
     1801 accacctcga ggccgacgtc ggcgaccagc tcctcgacct tgccgccatt gagcgtgatg
     1861 tgcgccttgg tggtggtgaa gtggtagttc atcggcacca ccgagcccgg ggacaccagc
     1921 acctgggcga ggatgttctc gcacgcgcgg ccctggtggg cgggcaggaa gaagggcatg
     1981 ccgaagatct cgtgcagctt gttctccagc cgggtgtagg tggcgctccc ggcgtagctg
     2041 tcgtcggcga tcatcatcgc cgccagctgc tggtcgctca tcgcattgac gccgctgtcg
     2101 gtgagcatgt ccatgaacac gtcggcgttg cgcagcagga aggtgttgtt gcccgcctcg
     2161 gtgatcgcct cgagccggcg ctcgaccggc ggcaggttca gcttctggac gatgcggacc
     2221 ttgtgcatct cgagcgggat cgactcttct ccgaagaact tcactttcgc catgacgggc
     2281 tgcctcctcg tgcgacttgc gtggatgaag gccgccatcg gaccacgacg gggatcagcg
     2341 gatgttgatg caacgcaaga gtcgttgacg cgaacggaaa catggccgcg gcatccgctt
     2401 tgcttcctga gcggaatcaa gggaaggaag cggccggact ggcacacttt ccatccgtgc
     2461 tccctcgacg cggccccgcg ccgcccgccc tgccgtgacc cttgccgtgc ccctctcccc
     2521 gaccgtcccc gtcccgactc cggcgccccg cccgccgcag gcgactgccg ggcgcatccc
     2581 cggcagcaag gcgatgtggg tgggaatctt ctgcgagatt accgagttcg ccctgctgtt
     2641 cgcggtcttc ttcatcgccc gcgcccacca cccggaggcc ttccatgccg ggcccgcacg
     2701 gctgtcgctg cttgccggca ccggctatac cctgatgctg ctcaccagcg gctggtgcgt
     2761 ggcgcgcgcg ctgcacgcaa tgcgacgcgg cgaccggcgc gcgacggtac gctggatgct
     2821 cgccggcttc gtgttcgggc tgggctaccc tgcgatcaag atcttcgagc tgcagcgcaa
     2881 ctttgcgctc gggctcgacg gcagcgccgg ggtattcatc gtcagctact actacctgac
     2941 cttcacccac ctggtgcatg tgttctgggg cctgctcggc atgatgtggg tgatcgcgcg
     3001 tacgggcctg ggcgcctaca cccccgaaga gcacagcggg ctggaggcct tcgcctgcta
     3061 ctggcacgtc accgacatga tctggctgat gatcttcccg ctcttctacc tgctcggatg
     3121 aggaaacccg catgagcccc gaacgcaaga tcgacctcgc ctggctgatg ctggtgttgc
     3181 tcagcgtggg cggcgcgggc atcgcccgtt ccgacgcgaa cgccgtgctg gtcgcggtgg
     3241 gcgtgtcctt ggtgatggcg atcaagctgc gtctggtgtg caactacttc ctcgagctcc
     3301 acgaagccca cccgcgcatc cgccgggccg tgctcgcctt ctgccacggc atgccgatcc
     3361 tggtcatcct caccaccgcc ttcggcgaca ccctctccag cctcaccggc gcgctgctcg
     3421 gctaggccgg ccgacggcgc agcaggccgc cgcctcgtgc aagccggccg cgctcaccag
     3481 tgcggcggaa tctcgtcttc cgggcatagc gggtcgcccg gctgcagggt cttgacctgc
     3541 tgggcgagct gatggaggtg ctggcgcagc aggtcgagtt cctgctgctg gcgccagacc
     3601 gtgcggttga gttcgtcgag gagatcctcg gcagccatca gcttggtctc cagcacgacg
     3661 atgcgctctt ccatggcggg cggtcctgaa tcggtttgcg ggcgcgcatt ctagccggcg
     3721 gcggcggcca ggcgggcgag cgcccaggcg acgtgctcgc gcaccaccgc atcctcgtcg
     3781 tccgcgcgcg cctgcaaggc cgcgcgcgcg gcgggcgtcg ccgggccgtt gccgagcgcg
     3841 accgcgatgt tgcgcagcca gcgcgcgtgg ccgatgcggt ggatcgggct gccggcggtg
     3901 cgctcggaga attcctccgc ggtccacgcg aagagctcgg ccagcctcgc gtcgtccagc
     3961 ccctggcggg gggcgaaatc gggctcccgg ccaagctggg cgaagcggtt ccacgggcac
     4021 acgagctggc agtcgtcgca gccgtagatg cggttgccga gtagcgggcg cagctcctcg
     4081 gggatcgcgc cgtgcagctc gatggtgagg taggagatgc agcgccgtgc gtcgagccgg
     4141 tagggcgcga cgatggcgcc ggtggggcag gcgtcgatgc aggccgtgca gcgcccgcag
     4201 tgcgacgcca ccggcgcgtc caccggcagc ggcaggtcgg tgaggatctc gccgaggaag
     4261 aaccacgaac cggcggtgcg atcgagcagc agggtgtgct tgccgcgcca gcccaggccc
     4321 ttgcgactgg cgtgctcgac ctcgaggatg ggcgccgagt cggtgaacac gcggtactgg
     4381 tgcggcacct ctgcgctgat gcggtcggcg agcttctgca ggcggttgcg caccagcttg
     4441 tggtagtcgc ggccgagcgc gtagcgcgac acataggcgc gctcggggtc gccgagcacg
     4501 tccatggccg gcgcggcatc cggccagtag cccatgcgca cgctgatcac gcgcacgctg
     4561 ccgggcagga gttcggcggc gcgcgcgcgt ttcatgccgt ggcgcaccat ataatccatg
     4621 tccccgtgga agccggcctc cagccaggcg accaggccgg gttcggcgtc ggcgagatcg
     4681 accccgccca cgccgaccgc gtcgaagccg agttcgcgcc cccactgtct gatccgcgca
     4741 acaagtgccg caccgtcgag gacggtcccg tcgaccgccg ccccgtccgg cgtcggcaat
     4801 tttgtttcca tttgtcagag gtcccgatga tcgagtcgat ccagactgcc gatgataacg
     4861 gcgcgcgcct gcaggcgcat ttacccgacg aggcggccac cgtggcgctt ggcggcgcgc
     4921 tcgccggcgt tctcgcgccc ggactgcaga tctggctgca gggcaacctg ggcaccggca
     4981 agaccaccct cacccgcggc ctgctgcgcg cgctcggcca cgagggcaag gtgaagagtc
     5041 cgacctacac cttgattgaa ccttacgtgg tttctagctt aaacttatat cactttgatt
     5101 tttatcgctt cacctcgccc gacgaatatc tcgacgcggg gctggacgaa tacttcgccg
     5161 gtgagggcgt ctgcatcgtg gaatggccgg acaaggcctc gccccatctg ccctctcccg
     5221 acgtcgaaat cgtcctgcag gccggtgaat ccgggcggga tgtggcgatc agcgcaaaca
     5281 cggaagcggg gcgaacatgc gtgatcggac tggtgaaagc tttgcggggc gcggacccgg
     5341 cgcccgggac gacctgagtc tcggccagac tccggggctt gccgccgcgg ccgaggcctt
     5401 cgccgcgggg cgcatcgatc gccgcggcct gctcaagttc gctggcgcct cgctcgccat
     5461 gctggtgagc ccggtcgggc tggcgagttc ggcaagcctg ctcgcggtgc gcgtatggcc
     5521 ctcggccgaa tacacccgca tcaccctcga gggctcctcg cgcctgcgcc acagccacat
     5581 gctggtcgag gatccgcagc gcctggtggt cgacctcgag ggcgtgcagc tcgacagcgt
     5641 gctgcagtcc ctgccctcga aggtactcga ctccgacccc tacatccgcc tgatccgtgc
     5701 cgggcagaac cggccgggcg tggtgcgtgt ggtgatcgag ctcaaggcgg cgatcaaccc
     5761 ccaggtgttc accctcgacc cggtcggcag ctacggccac cgcctcgtgc tcgacctgca
     5821 cccgaccgag gcccacgatc cgctgatggc gctgatcatg aaggactcgc cgatggacgc
     5881 cgcgatgggc gacgccggca gcaacaccac cgcggtggcg cgcgaggagc cgcgcgagcc
     5941 ggtgcgccgc ggcaggcgta acgagccggc ggtcgatcgc ctgtacaccg tggtgctgga
     6001 cgccggccat ggtggcgagg atccgggcgc gatcggtcgt ggcggcagct acgagaagga
     6061 cgtcacgctg tcgatcgcgc aacgcctcaa gcgcaagatc gacgccatgc ccggcatgcg
     6121 cgcggtgctc acgcgcgacg gcgactactt cgtgccgctg caccagcgcg tggcgcgcgc
     6181 ccgccgggtg cgcgccgatc tcttcgtgtc gatccacgcc gacgccttcg ttcgccccga
     6241 ggccaacggc agctcggtct atgtgctctc cgagcgcggc gcctcgagct cggccgcgcg
     6301 ctggctggca caaaaggaaa acgacgccga cctcgtgggc ggcgtcaacc tcgcccgcca
     6361 ggacggccac atcgcccgca ccctgctcga cctgtcgcag accgccacga tcaacgacag
     6421 cttcaagctc gggcgcgcca tgctcggcga gctcggcacc atcaaccggc tgcacaagcc
     6481 cgaggtggaa caggccggct tcgcggtgct gcgcgcaccc gacatcccct cggtgctggt
     6541 cgaaaccgcc ttcatcagca acccgcagga agagcgccgt ctcaacgacg aggcctacca
     6601 ggacaagatg gcgatggcgc tgatgcgcgg cgtcaagcgc tatttcgagg agcacgcccc
     6661 gagcgggccg acccgcgtgg cgcggctcga ctgagggcga gcgcaccgcc cgcatcccga
     6721 gcaagtctgc ccgcaaggcc cgcaatagcg ggctttgccg ctataatcgc ggctttttca
     6781 ggcgcttatg caggttcacc gcgggattcc cgagcaggcc gacacggctg cggtgctcac
     6841 catcggcaac ttcgatggcg tgcatctcgg ccaccaggcg ctgctttcgc tgctcaccga
     6901 caaggcccgt gcgctcggcc tgccggcggt ggtgctcacc ttcgagccgc acccgcgcga
     6961 atacttcagc cccgccgacg cccctgcccg cctcgcctcg ctgcgcgaga agctgctgct
     7021 gctttccgcc gccggcgtcg accgctgcca cgtctgccgc ttcgacgctc gcttcgccgc
     7081 tcagcccgcg caggccttca tcgacgacat cctggtgcgc ggcctcggcg tgcgccatct
     7141 cttcatcggt gacgatttcc gcttcggcgc ccgccgccag ggcaacttcg cgatgctgcg
     7201 cgcggccggc gccgagggcg gcttcggggt cgagtccatg cccacgctcg ccgtcgcggg
     7261 cgagcgggtg tccagctcgg cggtgcgggc cgcgctcgcg gagggcgacc tcgcccacgc
     7321 cgcgcgcctg ctcggccgcc cctacagcat cgccggtcgc gtcagccacg gcgacaagct
     7381 cggccgccag ctcggcttcc cgaccgcgaa catccagatg aagcaccgcc gcccggcgct
     7441 gggcggtgtc tacgtggtaa gcgtcgaagg cctcgcatcc ggcccggtgg ccggcgtcgc
     7501 caacatcggc ctgcgcccga ccgcgaccgc gaacggccgc gcccgcctcg aggtccacct
     7561 cttcgactgg acgcaggact gctacggcgc ccacatccgc gtacatttcc tgcatcgcct
     7621 gcgcgccgag caaaaattcc cctcgctcga cgcgctgcgc gcgcagatcg ccctcgacag
     7681 ccagacggcc cgcgactggc tggccgcgaa ccccctgccc gcctgaaggc ccgacgaacc
     7741 ccgccgatcc caccgacttc cgatcccgcc atggccgact accgcaagac gctcaacctg
     7801 cccgacaccc ccttcccgat gcgtggcgac ctgcccagac gcgagccgaa ctggatcacc
     7861 gactggcagc agaagaagct ctaccagcgc atccgcaagg ccagcgccgg ccggcccaag
     7921 ttcgtgctcc acgacggccc gccctacgcc aacggcagcc tgcacatcgg ccatgcactg
     7981 aacaagatcc tcaaggacat catcgtgcgc tcgaagacgc tggccggctt cgacgcgccc
     8041 tacgttccgg gctgggactg ccacggcctg ccgatcgagc acaaggtcga ggtgacgcac
     8101 ggcaagaacc tgcccgccga ccgcgtgcgt gagctgtgcc gcgcctacgc tgccgagcag
     8161 atcgagatcc agaaggccga cttcatccgc ctcggcgtgc tcggcgactg ggacaacccc
     8221 taccgcacga tggacttcgc caacgaagcc aacgagatcc gcgcgctcgc cgagatgacg
     8281 aagaacggct acgtgttcaa gggcctgaag ccggtgaact ggtgcttcga ctgcggctcg
     8341 gcgctggccg aggccgaggt ggaatacgcc gacaagccct cgccgacgat cgatgtcgcg
     8401 ttcccggtat ccgacgcgga cgccggcact catttgcgta aactcgccgc cgcattcggc
     8461 ctggccaggc tcgacaagcc cgccgccgcg gtgatctgga ccaccacccc gtggaccatc
     8521 cccgccaacc aggcgctcaa cgcccacccc gagttcgact atgcgctggt cgatgtcggc
     8581 gatcgtctgc tcgtgctcgc cagggaactc gtcgaatccg cgctgcagcg cttccagctc
     8641 gcaggcacca tcatcgccac cgccaagggc gcggcgctcg agcgcatcga gttccgccac
     8701 cccttctacg accgcgtctc gccggtgtat ctcgccgaat acgtcggcat cgacgccggc
     8761 accggcatcg tgcactcgtc gccagcgcac ggcgtggacg acttcaactc gtggcacgcg
     8821 tacggccgca gcaacgacga gatcctgtcc ctggtgacgg gcggcggcca gtacgtgtcc
     8881 gacctgccct tcttcggcgg catgaacatc tggaaggcca acccggcgat cgtcgacaag
     8941 ctcgccgagg tcggcgccct gctctcgcac ggcaagatca cccacagcta catgcactgc
     9001 tggcgccaca agacgccgct cgtctatcgc gccaccgcgc agtggttcgt cggcatggac
     9061 agggtcgccg ccgacggctc cacactgcgc gagcgcgcgc tgcgcgccgt ggaggccacc
     9121 aagttctacc ccgcgtgggg ccaggcgcgc ctgcacgcga tgatcgccaa ccgccccgac
     9181 tggtgcatct cgcgccagcg caact
//
LOCUS       DAOQNN010000093         9145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3735_length_9145_cov_9.033113, whole genome shotgun sequence.
ACCESSION   DAOQNN010000093 DAOQNN010000000
VERSION     DAOQNN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3735_length_9145_cov_9.033113"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKV97_06985"
     CDS             <1..199
                     /locus_tag="PKV97_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB05658.1"
                     /translation="LQPGYAGVRPKIVGPGEADADFRIDGPAAHGTPGLVHLYGIESP
                     GLTAALAIGEHVAALLAAEGR"
     gene            complement(237..>1210)
                     /locus_tag="PKV97_06990"
     CDS             complement(237..>1210)
                     /locus_tag="PKV97_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB05659.1"
                     /translation="KKTMLVSTGAEAVENAVKIARAFTGRPGVIAFNGAFHGRTLLAL
                     GLTGKVDPYKLGVGPFPGEIYHAPYPCALHGISVEDAIHGIELLFKNDIEARRVAAFI
                     VEPVQGEGGYYPAPAEFLQRLRALADKHGILLIADEIQSGIGRCGKMFAVEHSGVVPD
                     LITLAKGLGGGFPVAAVVGRAEVMDALAPGGLGSTYAGAPMACAAALAVLDVMEEEKL
                     CERALAIGEHMQRRLRDLAVRHKCIGDVRGLGAMVAVEFFHDGDHARPAPEIAKAVIA
                     AALQRGLLLLSCGSYGNVLRLMVPLTIEQAVLDEGLDLLAAAMDAAA"
     assembly_gap    1211..1310
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1383..2843)
                     /gene="gabD"
                     /locus_tag="PKV97_06995"
     CDS             complement(1383..2843)
                     /gene="gabD"
                     /locus_tag="PKV97_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNB05660.1"
                     /translation="MSLNLKDPDLFRTRCHVDGQWIDADDGATTSIRNPATGEVLGTI
                     PRMGAAETRRAIAAANAAWPAWRALTAGARAKILRRWFELILANQEDLAVLMTSEQGK
                     PLAEARGEVLYAASFIEWFAEEGKRIYGDVIPGHQPDKRIVVTKEPIGVCAAITPWNF
                     PAAMITRKAGPALAAGCTMVLKPATQTPYSALALAVLAERAGVPKGVFSVVTGGAAEI
                     GGELTANPIVRKLTFTGSTEIGVKLMAQCAPSVKKLSLELGGNAPFIVFDDADLDAAV
                     EGAIASKYRNTGQTCVCANRLLVQDGVYDAFAARLAAAVARLKVGNGLAEGSTQGPLI
                     DMNAVAKVEEHIADAVEKGARVLAGGKRHALGGSFFEPTILADVTPAMKVAREETFGP
                     VAPLFRFKDEAEAIRMANDTEFGLAAYFFANSMNRVWRVAEALEYGIVGINTGIISTE
                     VAPFGGMKSSGLGREGSKYGIEDYLEVKYLCMGGVQ"
     gene            complement(2920..3744)
                     /locus_tag="PKV97_07000"
     CDS             complement(2920..3744)
                     /locus_tag="PKV97_07000"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB05661.1"
                     /translation="MLQPYASPVERAWYYGMRGLVGLILAFLFLPILVIVPLSFNADS
                     FLMYPMSGFSLRWYEEFATSPAWRQALMNSLIVSPLATLLAMVLGTLASIGLVRADFP
                     GKALLTAVLISPMVVPVVVIGVAMYIFFAPLGLTGSYTGLVLAHAILGVPFVVTTVTA
                     TLQGFDFNLVRAASSLGANPLTTFFRVTLPMVAPGIISGGLFAFATSFDEVVVTLFLA
                     SPEQATLPRQMFAGIRENISPTIAAVATILIVLSTLMLMTLEWLRRRAERMRGARR"
     gene            complement(3756..5030)
                     /locus_tag="PKV97_07005"
     CDS             complement(3756..5030)
                     /locus_tag="PKV97_07005"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB05662.1"
                     /translation="MTTAVDTSAAVPLPAEGNGTPLKKRLRRAERRRNLLFGALILPL
                     ALVVLVAFIWPIGSLLTIGVDNPEIHDNLPRTLAALEDWDGRAPPAEAAFRALADELA
                     AAKGSPAIAAIAKRLNREDAGYRSLVMKTARKLPLDPAVPAREALLAIDAQWGELATW
                     QAIHRAGGALTPFYLLAAIDRTIDADGNIVAAPADERIYVDVLLRTFWMSLVVTVCCL
                     VLGFPVAYLMANLSTRHSNMLMVFVLLPFWTSVLVRVAAWIVLLQNEGLVNRLLMWLG
                     LTDSPLQLLFNRFGVYVAMVHILLPFMVLPLYSVMKGISPVYVRAALSLGCPPFRSFW
                     KIYFPQTLPGIGAGGLLVFIMCMGYYITPALLGGPKEQMLSYFIAFYTNQTINWGMAA
                     ALSAVLLAATLLLYMVYARYLGPTRVKYMKAR"
     gene            complement(5148..6188)
                     /locus_tag="PKV97_07010"
     CDS             complement(5148..6188)
                     /locus_tag="PKV97_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB05663.1"
                     /translation="MKHAASFLRAAVLVALGGAVGTASADITVISFGGASQKAQDKAY
                     YAPFTKATGVKVVAGEYNGEQAKVKAMVEAGNVTWDVLEVESPELVRGCEEGLFEKID
                     FAQVGDKADFVPAAVSECGVGIFVWSTALAYNADRLKEAPTSWADFWNVDKFPGKRGL
                     RKGAKYTLEFALLADGVPTSEVYKVLATPAGVDRAFAKLDKLKANIQWWEAGAQPPQL
                     LASGDLVMSAAYNGRISAAQVEGKNLKVVWNGSIYDVDSWTIPKGSPKKAEALKFIRF
                     ASQPENQAVFSGEIAYGPVNLKAVPAIDAKVAAGLPTAPENMKGAMATDTEFWVEHGE
                     NLEQRFNAWAAR"
     gene            complement(6276..7382)
                     /locus_tag="PKV97_07015"
     CDS             complement(6276..7382)
                     /locus_tag="PKV97_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB05664.1"
                     /translation="MEAVHNSQAAHVSFRNVQKTYDGETLIVRDLNLDIRRGEFLTLL
                     GPSGSGKTTCLMMLAGFETPTGGEIRLDGEVINRVPPYRRNIGMVFQNYALFPHMTVA
                     DNLRFPLRVRKLAANEVEARVKRALGMVQLEAFGNRYPQQLSGGQQQRIALARALVFD
                     PQLVLMDEPLGALDKNLRERMQLEIKHIQQNLGITVVFVTHDQSEALTMSDRIAVFNQ
                     GVIQQIDAPETLYEYPANSFVADFIGENNALDGTVVAVAGEECDVRLDCGTVLRARTG
                     NVGAGGRTRIAVRPERVQLAGEEGGTNRLDATVRELIYLGDQIRLRIELAGKHEFTIK
                     APVSQLDRSLRPGDAIRVGLDPAHTRALDVPAAH"
     gene            7503..8987
                     /locus_tag="PKV97_07020"
     CDS             7503..8987
                     /locus_tag="PKV97_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNB05665.1"
                     /translation="MDCRLSCELIAHAYAEQAPSSSARARLYLAIREAIIARRLAPGS
                     ALPPTRTLATDLALGRNTVVRVYDQLIVEGYLDSRVGSGTFVSDAVAATAPQQKPRRM
                     VGARSEGLSKRGCRIAADALSSRVQAGAFMPGFPDVTHFPFATWRKLVAKQMRLEQRH
                     LAQYGYGGYGPLKLVLAEYLRVTRMMSCSPQQILILNGSHQALDLCARMLADVGDVAW
                     MEDPGYWGARNVLSACDLAIHPVPVDAQGIHPQAEDWDHPPRLIFVSPSSQYPTGAVM
                     SLERRLRLLEYAEKNRIWIIEDDYDNEIRYHDHPVASLFGLSAAQRVIYVGTFSKVMY
                     PGLRLAYLVVPEDLVEAFTTGNAELYREGRMLEQAALAEFIDGGHFTTHIRRMRGIYE
                     ERRDRLIEVLRRHLAGIATPAGGLAGLHIPVHFERPLDDVLLSSQALREGVVMRPLSL
                     YAIDAANRRPGMNLGFAAVPVEQIEAPALKLVRLIHGLLPAGGR"
BASE COUNT         1386 a         3039 c         3244 g         1376 t
ORIGIN      
        1 cctgcagccg ggctatgccg gcgtgcgccc gaagatcgtc ggccccggcg aggcggacgc
       61 cgacttccgc atcgacggcc cggcggcgca tggcacgccg gggctggtcc atctctacgg
      121 catcgagtcg cccggcctca ccgcggcgct cgcgatcggc gagcacgtcg cggcgctgct
      181 cgcggccgag gggcgctgag tccgcagcgc tccccggcgc acaggtccga ccccgatcag
      241 gccgcggcgt ccatcgccgc ggcgaggagg tcgaggcctt cgtcgagcac cgcctgctcg
      301 atggtgagcg gcaccatcag gcgcagcacg ttgccgtagc tgccgcagga cagcagcaac
      361 aggccgcgct gcagcgccgc ggcgatcacc gccttggcga tctccggcgc cggacgcgca
      421 tggtcgccat cgtggaagaa ctccaccgcc accatcgcgc ccaggccgcg cacgtcgccg
      481 atgcacttgt ggcgcacggc caggtcgcgc aggcggcgct gcatgtgctc gccgatcgcg
      541 agcgcgcgct cgcacagctt ctcctcttcc atcacgtcga gcacggcgag cgcggcggcg
      601 caagccatcg gcgcgccggc ataggtgctg ccgaggccgc ccggggcgag cgcgtccatg
      661 acctcggcgc ggcccaccac cgccgcgacc ggaaagccgc cgccgaggcc cttggccagg
      721 gtgatgaggt cgggcaccac gccgctgtgt tcgaccgcga acatcttgcc gcagcggccg
      781 atgccggact ggatctcgtc ggcgatcagc aggatgccgt gcttgtcggc gagcgcgcgc
      841 aggcgctgca ggaactcggc gggcgcgggg tagtagccac cctcgccctg caccggctcg
      901 acgatgaagg cggcgacgcg acgcgcctcg atgtcgttct tgaacagcag ctcgatgccg
      961 tggatggcgt cctccacgct gatgccgtgc aacgcgcagg gatagggggc atggtagatc
     1021 tcgcccggga aggggccgac gccgagcttg taggggtcga ccttgccggt gaggccgagc
     1081 gcgagcagcg tgcggccgtg gaaggcgccg ttgaaggcga tcacgcccgg gcggccggtg
     1141 aaggcgcgcg cgatcttgac cgcgttctcg accgcctcgg cgccggtgct caccagcatg
     1201 gtcttcttcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttgtgcaggc
     1321 cgggggggat ggtgttggcg ttcatgggtg tctcctgtcg attcgcttgg ggggacggcg
     1381 gctcactgca cgccgcccat gcacagatac ttgacctcga ggtagtcctc gatgccgtac
     1441 ttggaacctt cgcggccgag gccggaggac ttcatgccgc cgaagggcgc gacctcggtc
     1501 gagatgattc cggtgttgat gccgacgatg ccgtactcga gcgcctcggc gacgcgccac
     1561 acgcggttca tcgagttggc gaagaaatag gcggcgaggc cgaactcggt gtcgttggcc
     1621 atgcggatcg cctcggcctc gtccttgaag cggaacagcg gcgccaccgg gccgaaggtc
     1681 tcctcgcgcg ccaccttcat cgccggggtc acgtcggcca ggatggtggg ctcgaagaag
     1741 ctgccgccga gcgcgtggcg cttgccgccg gcgagcacgc gcgcgccctt ctccaccgcg
     1801 tcggcgatgt gctcctcgac cttggccacc gcgttcatgt cgatcagcgg gccctgggtg
     1861 ctgccctcgg cgaggccgtt gcccaccttc aggcgcgcca ccgcggcggc gagcctggcg
     1921 gcgaaggcgt cgtacacacc gtcctgcacc agcaggcggt tggcgcacac gcaggtctgg
     1981 ccggtgttgc ggtatttgga agcgatcgcg ccctcgaccg cggcgtcgag gtcggcatcg
     2041 tcgaagacga tgaagggcgc attgccgccg agctcgagcg agagcttctt gacgctcggc
     2101 gcgcactgcg ccatcagctt gacgccgatc tcggtggagc cggtgaaggt gagcttgcgg
     2161 acgatcgggt tggcggtcag ctcgccgccg atctcggccg cgccgccggt gaccacgctg
     2221 aacacgccct tcggcacccc cgcgcgctcg gcgagcaccg cgagcgcgag cgccgagtaa
     2281 ggggtctggg tggcgggctt gagcaccatc gtgcatccgg ccgcgagcgc cggaccggcc
     2341 ttgcgcgtga tcatcgccgc ggggaagttc cacggcgtga tcgccgcgca caccccgatc
     2401 ggctccttgg tgacgacgat gcgcttgtcg ggctggtggc cggggatcac gtcgccgtag
     2461 atgcgcttgc cttcctcggc gaaccactcg atgaaggagg cggcgtagag cacctcgccg
     2521 cgcgcctcgg cgagcggctt gccctgctcg ctggtcatca gcacggcgag gtcttcctgg
     2581 ttggcgagga tgagctcgaa ccagcgccgc aggatcttcg cgcgcgcgcc ggcggtcagc
     2641 gcacgccacg ccggccatgc ggcgttggcg gccgcgatgg cgcgccgggt ctcggccgcg
     2701 cccatgcgcg ggatggtgcc gagcacctcg cccgtggccg ggttgcggat gctcgtggtg
     2761 gcgccgtcgt cggcgtcgat ccactgccca tcgacatggc agcgggtgcg gaagaggtcg
     2821 ggatctttca ggttcaggct catggtcggg gtccgtcggg tggtcggggg aaagcttggg
     2881 ttcttggagt cggtgccggg ggtggcgcga gcgcggcgct cagcggcgcg cgccacgcat
     2941 gcgctcggcg cgccggcgca accattcgag cgtcatcagc atcagcgtcg acagcacgat
     3001 caggatggtc gcgactgccg cgatggtcgg gctgatgttc tcgcggatgc cggcgaacat
     3061 ctgccgcggc agcgtcgcct gctcggggct ggcgaggaag agcgtgacca cgacctcgtc
     3121 gaaggaggtg gcgaaggcga acaggccgcc cgagatgatc ccgggcgcga ccatcggcag
     3181 ggtgacgcgg aagaaggtgg tgagcgggtt ggcgccaagg ctcgaggccg cgcgcaccag
     3241 gttgaagtcg aagccctgca gcgtcgcggt caccgtggtg acgacgaagg gcacgccgag
     3301 gatggcatgc gccagcacca ggccggtgta gctgccggtg aggccgagcg gggcgaagaa
     3361 gatgtacatc gccacgccga tcaccaccac cggcaccacc atcggcgaga tcagcaccgc
     3421 ggtgagcagc gccttgccgg gaaaatcggc gcgcaccagg ccgatcgagg ccagcgtgcc
     3481 gagcaccatc gccagcaggg tggcgagcgg cgacacgatc aggctgttca tcagcgcctg
     3541 gcgccaggcc ggcgaggtgg cgaactcctc gtaccagcgc agcgagaagc ccgacatcgg
     3601 gtacatcagg aagctgtcgg cgttgaagga cagcggcacg atgaccagga tgggcaggaa
     3661 caggaaggcc aggatcagcc cgaccagccc gcgcatgccg taataccatg cccgttccac
     3721 gggcgaagcg tagggttgca gcatcgtcgt cgtcctcagc gcgccttcat gtacttgacc
     3781 cgggtcggcc cgagatagcg ggcatagacc atgtacagca gcagggtcgc cgccagcagc
     3841 accgcggaaa gcgcggcggc catgccccag ttgatcgtct ggttggtgta gaaggcgatg
     3901 aagtagctca gcatctgctc cttcggaccg ccgagcagcg cgggcgtgat gtagtagccc
     3961 atgcacatga tgaacacgag caggccgccg gcgccgatgc cgggcagggt ctgcgggaag
     4021 tagatcttcc agaagctgcg gaagggcggg cagccgagcg agagcgcggc gcgcacatac
     4081 accggcgaga tgcccttcat caccgagtac agcggcagca ccatgaaggg cagcaggatg
     4141 tggaccatcg ccacatacac gccgaagcgg ttgaagagga gctggagcgg gctgtcggtg
     4201 aggccgagcc acatcagcag gcggttcacc aggccctcgt tctgcagcag cacgatccac
     4261 gccgccaccc gcaccagcac cgaggtccag aacggcagca gcacgaagac catcagcatg
     4321 ttgctgtggc gggtggacag attcgccatc aggtaggcca ccgggaagcc gagcaccagg
     4381 cagcacacgg tcacgaccag gctcatccag aaggtgcgca gcagcacgtc cacatagatg
     4441 cgctcgtccg cgggggcggc gacgatgttg ccgtcggcgt cgatcgtgcg gtcgatcgcg
     4501 gcgagcaggt agaacggcgt cagcgcgccg ccggcgcggt ggatcgcctg ccaggtggcg
     4561 agctcgcccc actgcgcgtc gatcgccagc agcgcctcgc gcgccggcac ggcaggatcg
     4621 agcggcagct tgcgcgcggt cttcatgacc aggctgcggt agccggcgtc ctcgcggttc
     4681 aggcgcttgg cgatcgcggc gatcgccggc gagcccttgg ccgcggcgag ctcgtcggcg
     4741 agcgcgcgga aggcggcctc ggccggtggc gcgcgaccgt cccagtcctc gagcgcggcc
     4801 agcgtgcgcg gcaggttgtc gtggatctcg gggttatcga caccgatcgt cagcagcgag
     4861 ccgatcggcc agatgaaggc caccagcacc accagcgcga gcggcaggat cagggcgccg
     4921 aagagcaggt tgcggcggcg ctcggcgcgg cgcaggcgct tcttcagcgg cgtgccattg
     4981 ccctcggcgg gcaagggaac ggcagcggag gtgtcgacgg cagtagtcat ggactcgtcc
     5041 actcgggcag atggagacgg tggccgcgcg ttgcggccgc cagcgcgggc cggacgcccg
     5101 cggcggcacg ggcatgcccg cgcacgggca tgcgacgaag gcagcgctca gcgcgcggcc
     5161 caggcgttga agcgctgctc gaggttctcg ccgtgctcga cccagaactc ggtatcggtc
     5221 gccatcgcgc ccttcatgtt ctcgggcgcg gtcggcaggc cggcggcgac cttggcgtcg
     5281 atcgccggca ccgccttcag gttgaccggg ccgtaggcga tctcgcccga gaacaccgcc
     5341 tggttctccg gctggctggc gaagcggatg aacttgagcg cctccgcctt cttcggcgag
     5401 cccttgggga tggtccacga atcgacgtcg tagatgctgc cgttccacac caccttgagg
     5461 ttcttgcctt cgacctgcgc ggcggagatg cggccgttgt aggcggcgct catgaccagg
     5521 tcgcccgagg cgagcagctg cggcggctgc gcgccggcct cccaccactg gatgttggcc
     5581 ttgagcttgt cgagcttggc gaaggcgcgg tcgacaccgg ccggggtggc gagcaccttg
     5641 tagacctcgc tcgtcggcac gccatcggcg agcagggcga actcgagggt gtacttcgcg
     5701 cccttgcgca gcccgcgctt gccggggaat ttgtctacgt tccagaagtc cgcccaggac
     5761 gtcggcgcct ccttgaggcg gtcggcgttg taggcgagcg cggtcgacca cacgaagatg
     5821 ccgacaccgc attcgctcac ggcggcaggg acgaaatcgg ccttgtcgcc cacctgcgcg
     5881 aagtcgatct tctcgaacag gccttcctcg cagccgcgca ccagctcggg cgactcgacc
     5941 tcgagcacgt cccaggtcac gttgccggcc tcgaccatgg ccttcacctt ggcctgctcg
     6001 ccgttgtact cgccggcgac gaccttgacc ccggtggcct tggtgaaggg cgcgtagtag
     6061 gccttgtcct gcgccttctg cgaggcgccg ccgaaggaga tgacggtgat gtcggccgat
     6121 gcggtgccga cggcgccccc gagggcgacg aggacggcgg cgcgaaggaa ggatgcggcg
     6181 tgcttcatga tgtgtctccg gttctggtta tgtgggtgct gcgggtgaag accggcggcc
     6241 ggccgggact gcggatgcgg cggtgggcgg atgcctcagt gcgccgcggg cacgtcgagc
     6301 gcccgcgtgt gcgccggatc gagcccgacg cggatcgcgt cgccggggcg cagcgagcgg
     6361 tcgagctgcg agaccggcgc cttgatggtg aattcgtgct tgccggcgag ttctatgcgc
     6421 aggcggatct ggtcgccgag atagatcagc tcgcgcacgg tggcgtcgag ccggttggtg
     6481 ccgccctcct ccccggcgag ctgcacgcgc tccgggcgca ccgcgatgcg ggtgcggccg
     6541 ccggcgccga cgttgccggt gcgcgcgcgc agcaccgtgc cgcagtccag gcgcacgtcg
     6601 cactcctcgc ccgccaccgc gaccacggtg ccgtcgagcg cgttgttctc gccgatgaag
     6661 tcggcgacga aggagttcgc ggggtattcg tacagggtct cgggagcgtc gatctgctgg
     6721 atgaccccct gattgaacac cgcgatgcgg tccgacatgg tcagcgcctc gctctggtcg
     6781 tgggtgacga acaccaccgt gatgccgagg ttctgctgga tgtgcttgat ctcgagctgc
     6841 atgcgctcgc gcaggttctt gtcgagcgcg ccgagcggct cgtccatcag caccagctgg
     6901 ggatcgaaca cgagcgcgcg ggcgagcgcg atgcgctgct gctggccgcc ggagagctgc
     6961 tgcgggtagc ggttgccgaa ggcctcgagc tgcaccatgc cgagcgcgcg cttcacccgc
     7021 gcctcgacct cgttcgccgc cagcttgcgc acgcgcagcg ggaagcgcag gttgtcggcc
     7081 accgtcatgt gcggaaacag cgcatagttc tggaacacca tgccgatgtt gcggcggtag
     7141 ggcggcaccc ggttgatgac ctcgccgtcg aggcggatct cgccaccggt gggcgtctcg
     7201 aagccggcga gcatcatcag gcaggtggtc ttgcccgaac cggacggccc gagcagggtg
     7261 aggaactcgc cgcggcgaat gtcgagattc aggtcccgca cgatgagggt ttcgccgtcg
     7321 taggtcttct ggacgttgcg aaagctgacg tgggctgcct ggctgttgtg gaccgcttcc
     7381 atctgtctcc gtctcctgca aggggcgctg tgcgctctgg cgatcgaatc ctaggccacg
     7441 aattggccca ggcttgatcc aattacttat aatttgatag aaccaatttt tgctgcactg
     7501 caatggattg ccgactcagc tgcgagctca tcgcccacgc ttatgcggaa caggcgcctt
     7561 cgtccagcgc ccgcgcgcgc ctctacctcg ccatccgcga ggccatcatc gcgcgccgcc
     7621 tcgcccccgg cagcgcgctg ccgcccaccc gcaccctggc gacggatctg gccttggggc
     7681 gcaacaccgt ggtgcgggtg tacgaccagc tcatcgtcga gggctatctg gacagccggg
     7741 tgggctcggg caccttcgtc tccgacgccg tggccgctac cgcgccgcag caaaaaccgc
     7801 ggcggatggt cggcgcgcgc agcgagggat tgtcgaaaag ggggtgccgg atcgccgccg
     7861 atgcgctgtc gagccgcgtg caggcgggcg cattcatgcc cggcttcccg gacgtcaccc
     7921 attttccctt cgccacctgg cgcaagctgg tcgccaagca gatgcggctc gagcagcgcc
     7981 acctcgcaca gtacggctac ggcggctacg ggccgctgaa gctggtgctg gccgaatacc
     8041 tgcgcgtgac ccgcatgatg tcgtgcagcc cgcagcagat cctgatcctg aacggctcgc
     8101 accaggcgct cgacctgtgc gcgcgcatgc tcgccgacgt cggcgacgtg gcgtggatgg
     8161 aggatccggg ctactggggg gcacgcaacg tgctgtccgc ctgcgatctc gccatccacc
     8221 cggtgccggt ggacgcgcag ggcatccatc cgcaggccga ggactgggac catccgccgc
     8281 gcctgatctt cgtgtcgccc tcgagccagt acccgaccgg ggcggtgatg tcgctcgaac
     8341 gccggctccg cctgctcgaa tacgccgaga agaaccgcat ctggatcatc gaggacgact
     8401 acgacaacga gatccgctac cacgaccacc cggtggcctc gctgttcggc ctgtcggcgg
     8461 cgcaacgggt gatctacgtc ggcaccttca gcaaggtgat gtatcccggc ctgcgcctgg
     8521 cctacctggt ggtgccggag gatctcgtcg aggccttcac caccggcaac gccgagctct
     8581 accgcgaggg ccgcatgctc gagcaggcgg cgctggccga gttcatcgac ggcggccatt
     8641 tcaccaccca catccgccgc atgcgcggca tctacgagga gcgccgcgac cgcctcatcg
     8701 aggtgctgcg gcgccacctc gccggcatcg ccacgccggc cggcggcctc gccggcctgc
     8761 acatcccggt ccacttcgag cgcccgctcg acgacgtgct gctctcatcg caggcgctgc
     8821 gcgagggcgt ggtgatgcgg ccgctgtcgc tctacgcgat cgacgccgcc aaccggcgcc
     8881 ccggcatgaa cctcggcttc gcggcggtcc cggtggagca gatcgaggcc ccggcactca
     8941 agctggtgcg gctgatccac ggcctcctgc cggcaggagg ccgctgaacg cggacgaaaa
     9001 aaatgcggcc gccggtcgaa acggaccgga cgagccgcag agggggagag gcgggtagga
     9061 ctcaggccgc cgcaacgagc gcggggactg cgtcgaaaag gtcggccacg aggccgtagt
     9121 cggcgacctg gaagatcggc gcttc
//
LOCUS       DAOQNN010000094         9064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3790_length_9064_cov_9.621712, whole genome shotgun sequence.
ACCESSION   DAOQNN010000094 DAOQNN010000000
VERSION     DAOQNN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3790_length_9064_cov_9.621712"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="PKV97_07025"
     CDS             complement(<1..1406)
                     /locus_tag="PKV97_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNB05666.1"
                     /translation="MKAAEAGPAPEVFRPPVSSVLALDFQQLKTKLQTYLRPEDVGRV
                     EAAYHFSADAHAGQFRVSGEPYVSHPVAVCSLVADWHLDPQALIAALLHDVMEDTHIT
                     KAEIAERFGKVAAELVDGLSKLDKIEFRSQEEAQAENFRKMLLAMASDLRVILIKLAD
                     RLHNMRTLDHMRPAKRRRIANETLEIYAPIANRLGLNDLFRELQELSFRNKYPLRFEV
                     LAKAIRSARGNRREVVGKILASIEERLPQWGIAAEVQGREKHLYGIYRKMVEKHLSFS
                     QVLDIYGFRVIVKDVPSCYLALGALHSMYKPVPGKFKDYLAIPKANGYQSLHTTLIGP
                     FGMPVEVQIRTREMHHIAETGVASHWLYKEDEKTLTELQQKTHSWLQSLLELQSASGE
                     ATEFLEHVKIDLFPGEVYVFSPKGKIFSMPKGSTPVDFAYAVHTDVGNRCVACRINGD
                     LMPLRSELRNGDQVEIITA"
     gene            complement(1424..1639)
                     /gene="rpoZ"
                     /locus_tag="PKV97_07030"
     CDS             complement(1424..1639)
                     /gene="rpoZ"
                     /locus_tag="PKV97_07030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNB05667.1"
                     /translation="MARITVEDCLKRIPNRFQLTLAATYRARQLTAGGTPQIDVDPLD
                     RDKPTVIALREIEAGKVGLEMLNRGQA"
     gene            complement(1642..2256)
                     /gene="gmk"
                     /locus_tag="PKV97_07035"
     CDS             complement(1642..2256)
                     /gene="gmk"
                     /locus_tag="PKV97_07035"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNB05668.1"
                     /translation="MAGTLFIVTAPSGAGKTTLVRGLLQRDPRVQLSISYTTRAPRPG
                     EQDGREYNFVDVPTFRALRDRGEFLEWAEVHGNYYATSRVWLKQQVEAGRDTLLEIDW
                     QGAQQVRKHFPDAVGVFILPPSMDELEARLRGRNTDSDEVISRRLLGARGEMRHVAEF
                     DYVIINDEIDAALDDLVAVVRAARLRCAKQHQRHPEYFAFLEQD"
     gene            complement(2389..>2784)
                     /locus_tag="PKV97_07040"
     CDS             complement(2389..>2784)
                     /locus_tag="PKV97_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNB05669.1"
                     /translation="VKALALADVGNASKPHALIREDNAVAYDPAAGWKKGDVLPGRLL
                     SRADASGSAADNADVRGEWADGQWTVLWTRKLDTGHADDDKALKPGGVVNVGFAVHDD
                     NVTTRFHHVSFPLTLGVGTKATISSVALE"
     assembly_gap    2785..2884
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2885..3564)
                     /locus_tag="PKV97_07045"
     CDS             complement(<2885..3564)
                     /locus_tag="PKV97_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNB05670.1"
                     /translation="MTTSTWSASGAAAFAVCVAAAATPAFAAAPADWAAIPVQTVTLF
                     YPGQVDYGWLRSPEHKRANAKVKAGEACLSCHEGEEAELGATLVKGGRHEPVPIAGKL
                     GVVALQVQAAHDDANLYLRFQWKTQMARAGQMHDYMMYDGEKWVFIGGPRSKEAVRSG
                     AQPPLYEDRLSVMIDDGKVPMFANQGCWLTCHTGMRDMPGEPTKEQVQAHPLIGQTHK
                     ESDVRKYLP"
     gene            complement(3781..4647)
                     /locus_tag="PKV97_07050"
     CDS             complement(3781..4647)
                     /locus_tag="PKV97_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNB05671.1"
                     /translation="MVQSMTGFAVQTRDLGSVSLHLELRSVNSRYLDLGFRIVDDLRA
                     AEPAIRERISARLGRGKVECRLNLQAGHAAPRSMALNGALLDQLAAAQDGLRARFPDA
                     APLSVNELLRWPGMLADDSLGFDEMLPAIVELTNTALDELVATRQREGDKLADMIRER
                     VARMRELVAQAAPRMPAVVLEYQERLTAKLRDAVASLDEDRVRQEVALYAQRIDVDEE
                     LTRLSTHLDEVERILKSAGAAGKRLDFLMQELNREANTLASKSPATDITAIAMEMKVL
                     IEQMREQVQNLE"
     gene            4739..5686
                     /locus_tag="PKV97_07055"
     CDS             4739..5686
                     /locus_tag="PKV97_07055"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB05672.1"
                     /translation="MPSQANCALPPGFQLDQYRIERQLSLGGFSIVYLARDAHGMAVA
                     IKEYLPNSLALRKEGETEPQVSEEHRLAFRYGMKCFFEEGRSLAKLMHPNVVRVLNFF
                     RANGTVYMVMQFERGRTLHDYIRKHRGEVKEMLIRAVFARMLNGLREVHAHKLMHLDI
                     KPSNIYLRNDGTPVLLDFGAARQTLMSDQPVLKPMYTPGYAAPEQYEKGAQLGPWTDI
                     YSVGASLHACIVGSPPPRADERAQEDTLQPLARTQAGRYSRQLLELVDWCLHLDPQQR
                     PPSVYALQKALIHQENAPERPEHWFSDLGSRLRSFIGRS"
     gene            5696..6619
                     /locus_tag="PKV97_07060"
     CDS             5696..6619
                     /locus_tag="PKV97_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HNB05673.1"
                     /translation="MRFTIYQESRPGKRRDNQDRIAHCYSRDALLMLVADGMGGHLHG
                     EVAAQIAVQCIAERFQAEARPLLPDPQLFLARAIERAHHAILDHALDRQLPEAPRTTL
                     VACVIQRGAAYWAHAGDSRLYLLRRGHLVTRTRDHSRTQLMIDQGVIKPEEAHLHPGR
                     NRIYSCLGGNYNPQVEYSPRTPLRDGDLLALCTDGVWGPIDDDGLVVNLTAGVLSRAV
                     PRLMDIAERKAGASADNLSMVAMCWHDENAVGAATVESISTRTMELDGFESRLDLSGP
                     GPTAVAELDEDEIERAIREINAAIHKFDSRS"
     gene            6629..7360
                     /gene="rph"
                     /locus_tag="PKV97_07065"
     CDS             6629..7360
                     /gene="rph"
                     /locus_tag="PKV97_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNB05674.1"
                     /translation="MRPSLRQPDQLRAVRLTRGFTCHAEGSVLVEFGATRVLCTASVE
                     ETVPGFLRGKGQGWVTAEYGMLPRATHTRSAREAAKGKQSGRTQEIQRLIGRSLRAVV
                     DLGALGERQIVVDCDVLQADGGTRTAAITGACVAVHDALVRLVAAGKLARNPMREFVA
                     AVSVGIHQGVPVLDLDYAEDSDCDTDMNVVMTEGGGLIEVQGTAEGTPFSRAELDALL
                     DLAELGGRQLFELQRGAVRSAIFTA"
     gene            7397..8002
                     /gene="rdgB"
                     /locus_tag="PKV97_07070"
     CDS             7397..8002
                     /gene="rdgB"
                     /locus_tag="PKV97_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNB05675.1"
                     /translation="MSKRLVLASNNAKKAAEMQALLAPLGIEVIPQSVFGVGEAEEPH
                     PSFVENALAKARHASAATGLPAVADDSGLCVEALGGAPGVISARFAGEPRSDARNNAL
                     LLEKLAHLPEPAQRRAYFYSAVVLVRHAEDPRPLIADGEWHGEILPAARGEGGFGYDP
                     LFWVPELEQTAAELDSALKNTLSHRGAAMRHLLDRLAEHPL"
     gene            8037..>9064
                     /gene="hemW"
                     /locus_tag="PKV97_07075"
     CDS             8037..>9064
                     /gene="hemW"
                     /locus_tag="PKV97_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNB05676.1"
                     /translation="MTTRRIIPLTPATGAARALPLPERPQLEAPPPLSLYVHFPWCVK
                     KCPYCDFNSHAPREGGIPEQAWLAAVLADLQHALPQVWGRRVHSVFIGGGTPSLMSAA
                     TLDGLLTGIRMLLPLDPLAEITLEANPGTVEAGRFRDYRAAGVNRLSLGIQSFDDAML
                     AKIGRIHGGEEARRAIEAARSHFERVNLDLMYALPGQTLDMALADLETAIGFGVQHLS
                     CYHLTLEPNTPFAHNPPPLPDDDTAADMQEAIEARLAAAGFTHYETSAFARPHEQSRH
                     NLNYWTFGDYLGLGPGAHGKLSSHEGIRREMRHKHPGRYLEGAARGDFIQEAREVSVA
                     ELPFEFMM"
BASE COUNT         1407 a         3181 c         3010 g         1366 t
ORIGIN      
        1 gcggtgatga tctcgacctg gtcgccattg cgcagctcgc tgcgcagcgg catcaggtcg
       61 ccgttgatgc ggcaggcaac gcagcgattg ccgacgtcgg tatgcaccgc gtaggcgaag
      121 tcgaccgggg tcgagccctt gggcatcgag aagatcttgc ccttgggcga gaacacgtag
      181 acctcgccgg ggaagaggtc gatcttgacg tgctcgagga actcggtggc ctcgcccgag
      241 gccgactgca gctcgagcag ggactgcagc caggagtgcg tcttctgctg cagctcggtg
      301 agggtcttct cgtcttcctt gtacagccag tgcgaggcca cgccggtctc ggcgatgtgg
      361 tgcatctcgc gggtgcggat ctgcacctcg accggcatgc cgaagggacc gatcagggtg
      421 gtgtgcaggc tctggtagcc gttggccttg gggatggcca gatagtcctt gaacttgccc
      481 ggcaccggct tgtacatcga gtgcagcgca ccgagcgcga ggtagcagct cggcacgtcc
      541 ttgacgatga cgcggaagcc gtagatgtcg agcacctgcg agaaggacag gtgcttctcg
      601 accatcttgc ggtagatgcc gtagaggtgc ttctcgcgcc cctgcacctc ggcggcgatg
      661 ccccactgcg gcaggcgctc ctcgatgctg gcgaggatct tgccgaccac ctcgcggcgg
      721 ttgccgcgcg ccgagcggat cgccttggcg agcacctcga agcgcagcgg gtacttgttg
      781 cggaaggaca gctcctgcag ctcgcggaag aggtcgttga gcccgagccg gttggcgatc
      841 ggcgcgtaga tctcgagcgt ctcgttggcg atgcggcggc gcttggccgg acgcatgtgg
      901 tccagcgtgc gcatgttgtg cagacggtcg gcgagcttga tgaggatgac gcgcaggtcg
      961 ctcgccatcg ccagcagcat cttgcggaag ttctccgcct gcgcctcttc ctgcgagcgg
     1021 aactcgatct tgtcgagctt ggagaggcca tcgaccagct cggcggcgac cttgccgaag
     1081 cgctcggcga tctccgcctt ggtgatgtgg gtgtcctcca tcacgtcgtg gaggagcgcg
     1141 gcgatcagcg cctgcgggtc gaggtgccag tcggcgacca gcgaacacac cgccaccggg
     1201 tggctgacat agggctcgcc gctgacgcgg aactggccgg cgtgggcatc ggcggagaag
     1261 tggtaggccg cctcgacgcg gcccacgtcc tcgggccgga ggtaggtctg cagcttggtc
     1321 ttgagctgct ggaagtcgag cgcgagcacg ctcgacaccg gcgggcggaa gacttcgggg
     1381 gctgggcctg cttcggcggc tttcatgcaa cccccgggcg atttcaggcc tggccgcggt
     1441 tgagcatctc gaggccgacc ttgcccgctt cgatctcgcg cagcgcgatc acggtcggct
     1501 tgtcccggtc gagcggatcg acgtcgatct gcggcgtgcc gccagcggtg agctggcggg
     1561 cacgataggt cgccgccagg gtgagctgga agcggttcgg gatcctcttg aggcagtctt
     1621 ctacggtgat gcgggccatg ttcaatcctg ttcgagaaag gcgaagtact cgggatgccg
     1681 ctggtgctgc ttggcgcagc gcaaacgcgc ggcgcgcacc acggcgacga ggtcgtcgag
     1741 tgcggcatcg atttcgtcgt tgattataac gtagtcgaat tccgcgacat ggcgcatttc
     1801 cccgcgcgcg ccgagcaacc tgcggctgat gacctcgtcg ctgtcggtat tgcgaccgcg
     1861 caggcgggcc tcgagttcat ccatcgaggg cggcaggatg aagaccccga ccgcgtccgg
     1921 gaagtgcttg cgcacctgct gcgcgccctg ccagtcgatc tcgagcaggg tatcgcgccc
     1981 ggcctcgacc tgctgcttga gccacacgcg cgaggtggcg tagtagttgc cgtgcacctc
     2041 ggcccattcg aggaactcgc cgcggtcgcg caaggcacgg aaggtgggca cgtcgacgaa
     2101 gttgtactcg cgcccgtcct gctcgccggg gcgcggcgcg cgcgtggtgt aggagatcga
     2161 cagctgcacg cgcgggtcgc gctgcagcaa gccgcgcacc agggtggtct tgccggcgcc
     2221 cgagggcgcg gtgacgatga aaagggttcc ggccatggtc gttgtctcag ggccggtgca
     2281 gcgcaccggc agagggatgg gaggcggccg acgcagcaga acggccgccg aggggagccg
     2341 ccgggcgggc ggcagcgtgc tgcaaccgca agcgttgccg gaaggcgctc actcgagcgc
     2401 gaccgaggag atggtggctt tcgtgccgac ccccagggtc agcgggaagg acacatgatg
     2461 gaaacgcgtt gtgacgttgt cgtcatgaac ggcaaagccc acgttgacga cgccgcccgg
     2521 cttcagggcc ttgtcgtcgt cggcatgccc ggtgtcgagc ttgcgcgtcc acagcaccgt
     2581 ccactgacca tccgcccact cgccgcggac gtcggcattg tccgccgccg aaccgctggc
     2641 gtcggcgcgt gagagcaggc gcccgggaag gacgtcgccc ttcttccagc ccgcggccgg
     2701 atcgtaggcc acggcgttgt cttcccggat cagcgcgtgc ggcttcgacg cgttgccgac
     2761 atccgcgagc gcaagcgcct tcacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnggcaga tacttgcgca cgtcgctttc cttgtgcgtc tggccgatca gcggatgggc
     2941 ctggacctgc tccttggtcg gctcgccggg catgtcgcgc atgccggtgt ggcaggtcag
     3001 ccagcacccc tggttggcga acatcggcac cttgccgtcg tcgatcatca ccgacaggcg
     3061 atcctcgtag agcggaggct gggcgccgct gcgcaccgcc tccttcgagc gcggcccgcc
     3121 gatgaagacc cacttctcgc cgtcgtacat catgtagtcg tgcatctgcc cggcccgcgc
     3181 catctgcgtc ttccactgga agcgcaggta caggttggcg tcgtcatggg cggcctggac
     3241 ctggagcgcg accactccga gcttgccggc gatcgggacc ggttcgtgcc gcccgccctt
     3301 gacgagggtg gcgccaagct ccgcttcctc gccctcgtga caggacagac aggcctcgcc
     3361 cgccttgacc ttggcattgg cacgcttgtg ctccgggctg cgcagccagc cgtaatcgac
     3421 ctgcccggga tagaacagcg tcaccgtctg caccgggatg gccgcccagt cagcgggagc
     3481 cgcggcgaag gccggtgtcg ctgcagcagc cacacaaacg gcgaatgctg cggcgccgga
     3541 agcactccat gtcgatgtag tcatcatttc tctccgtctg atccggggac aaacatgcgc
     3601 ggaggctgcc gggcgctggc gattgcagcg ctgccgtgcc cgcccgcaca agggtctcca
     3661 ggccagccgg aaaaaacctg aagcggccgg gtggaggccc tcttgttacc acgaacggca
     3721 cgtgcgcgat agaccggcac ccggcccttc cagctccccg ctcccgggga cggtcattcc
     3781 tcactcgagg ttctgcacct gctcgcgcat ctgctcgatc agcaccttca tctccatcgc
     3841 gatcgcggtg atgtcggtgg cgggcgactt ggaggccagg gtgttggcct cgcggttgag
     3901 ctcctgcatg aggaagtcga gccgcttgcc ggcggcgccg gcgctcttca ggatgcgctc
     3961 gacctcgtcg aggtgggtgg acaggcgggt gagctcctcg tccacgtcga tgcgctgggc
     4021 gtagagcgcg acctcctggc ggacgcggtc ctcgtccagg ctggcgacgg cgtcgcgcag
     4081 cttggcggtc aggcgctcct ggtactcgag caccaccgcc ggcatccgcg gggccgcctg
     4141 ggcgacgagc tcgcgcatgc gcgcgacgcg ctcgcggatc atgtcggcga gcttgtcgcc
     4201 ctcgcgctgg cgggtggcga caagctcgtc gagcgcggtg ttggtcagct cgacgatcgc
     4261 cggcagcatc tcgtcgaagc cgaggctgtc gtcggcaagc atgcccggcc aacgcagcag
     4321 ctcgttgacc gacagcggcg cggcgtcggg gaagcgcgca cgcaagccgt cctgcgcagc
     4381 ggcgagctgg tcgagcagcg cgccgttgag cgccatgctg cgcggcgcgg cgtggccggc
     4441 ctgcaggttg aggcgacatt cgaccttgcc gcggccgagg cgcgcggaga tgcgctcgcg
     4501 gatcgcgggc tccgcggcgc gcaggtcgtc gacgatgcgg aagccgagat cgagataacg
     4561 cgagttgacg ctgcgcagct cgagatgaag gctgacacta cccaggtctc gcgtctgaac
     4621 cgcgaaacct gtcatgctct ggaccatggg gcgctccggg gaccgcgccc gatcggataa
     4681 actgggcgcc tggatcagga atgaaaccgc gaaaacagcg attctagcgc tctcgaagat
     4741 gccgtctcaa gccaactgcg cgctgccacc ggggtttcag ctcgaccagt accgcatcga
     4801 gcggcaactg tcgctgggcg gcttctcgat cgtctatctc gcgcgcgacg cgcatggcat
     4861 ggccgtggcg atcaaggaat acctgcccaa ctcgctcgcc ctgcgcaagg aaggcgagac
     4921 cgaaccgcag gtcagcgagg agcaccgcct cgccttccgc tacggcatga agtgcttctt
     4981 cgaggaaggc cgctcgctcg ccaagctgat gcatccgaac gtggtgcggg tgctcaactt
     5041 cttccgcgcc aacggcacgg tgtacatggt catgcagttc gagcgcggcc gcaccctgca
     5101 cgactacatc cgcaagcacc gcggcgaggt caaggagatg ctgatccgcg cggtgttcgc
     5161 gcgcatgctc aatggcctgc gcgaggtgca cgcgcacaag ctcatgcacc tcgacatcaa
     5221 gccgtccaac atctacctgc gcaacgacgg caccccggtg ctgctcgact tcggcgccgc
     5281 gcgccagacc ctgatgagcg accagccggt gctcaagccg atgtacacgc ccggatacgc
     5341 cgcgccggaa caatacgaga agggcgccca gctcggtccg tggaccgaca tctacagcgt
     5401 gggtgcgagc ctgcacgcct gcatcgtcgg cagcccgccg ccgcgcgcgg acgaacgcgc
     5461 gcaggaagac acgctccagc ccctggcgag gacccaggcc gggcgctaca gccggcagct
     5521 gctcgagctc gtcgactggt gccttcacct cgacccgcag cagcgcccgc ccagcgtgta
     5581 tgcgctgcag aaggcgctga tccatcaaga aaacgccccc gagcgtccgg aacactggtt
     5641 ctcggacttg ggcagccggc tgcgctcctt catcggccgc agctgaggac ccgccatgcg
     5701 cttcaccatc taccaggaaa gccgccccgg caaacgacgc gacaaccagg accgcatcgc
     5761 ccactgctac tcgcgcgacg ccctgctgat gctggtcgcc gacggcatgg gcggccacct
     5821 gcacggcgag gtggcggcgc agatcgcggt gcagtgcatc gcggagcgct tccaggcgga
     5881 ggcgcggccg ctgctgcccg acccccagct gttcctcgcg cgcgcgatcg aacgcgccca
     5941 ccacgccatc ctcgaccatg cgctcgaccg ccagctgccg gaggcgccgc gcaccaccct
     6001 ggtcgcgtgc gtgatccagc gcggcgcggc gtactgggcg cacgccggcg attcgcggct
     6061 ctacctgctg cgccgcggcc acctggtcac gcgcacgcgc gaccattcgc gcacccagct
     6121 gatgatcgac caaggcgtga tcaagccgga ggaggcccac ctccaccccg ggcgcaaccg
     6181 catctattcc tgcctcggcg gcaactacaa cccgcaggtg gagtattctc cgcgcacccc
     6241 gctgcgcgac ggcgacctgc tcgcgctctg cacggacggc gtgtggggcc cgatcgacga
     6301 cgacggcctg gtggtgaacc tcaccgccgg cgtgctcagc cgggcggtgc cgcggctgat
     6361 ggacatcgcc gagcgcaagg ccggcgccag cgccgacaac ctgtcgatgg tggcgatgtg
     6421 ctggcacgac gagaacgccg tcggcgccgc gaccgtcgaa tcgatctcca cccgcaccat
     6481 ggaactggac ggcttcgagt cccgcctgga cctctccggc ccgggtccga cggccgtcgc
     6541 cgaactcgac gaggacgaga tcgagcgcgc catccgcgag atcaacgccg ccatccacaa
     6601 attcgactcc cgctcctagg acctcacgat gcgacccagc ctgcgccagc ccgaccaact
     6661 ccgcgccgtc cgcctcaccc gcggcttcac ctgccacgcc gaaggctcgg tgctggtcga
     6721 attcggcgcc acccgcgtgc tgtgcaccgc cagtgtcgag gagacggtac ccggcttcct
     6781 gcgcggcaaa ggccagggat gggtgaccgc cgaatatggc atgctgccgc gcgccactca
     6841 cacgcgcagc gcgcgcgagg ctgccaaggg caagcagagc gggcgcaccc aggagatcca
     6901 gcgcctgatc gggcgcagcc tgcgcgcggt ggtcgacctc ggcgcgctcg gcgagcgcca
     6961 gatcgtcgtc gactgcgacg tgctgcaggc cgacggcggc acgcgcaccg cggccatcac
     7021 cggcgcctgc gtcgcggtgc atgacgccct ggtccgcctg gtcgccgccg gcaagctcgc
     7081 gcgcaacccg atgcgcgagt tcgtcgccgc ggtctcggtc ggcatccacc agggcgtgcc
     7141 ggtgctcgac ctcgactacg ccgaggactc cgactgcgac accgacatga acgtggtgat
     7201 gaccgagggc ggcggcctga tcgaggtcca gggcacggcc gagggcaccc cgttctcgcg
     7261 cgccgagctg gacgccctgc tcgacctcgc cgaactcggg ggccggcagc tgttcgaatt
     7321 gcagcgtggc gcggtgcgct cggccatctt cacggcctga gcccggcccg cgccaggctt
     7381 cgccaggaga acaagcatga gcaaacgact cgtcctcgcc agcaacaacg ccaagaaggc
     7441 cgccgagatg caggccctgc tcgccccgct cggcatcgag gtgatcccgc agtccgtgtt
     7501 cggcgtcggc gaagccgagg agccccaccc gagcttcgtc gagaacgcgc tcgccaaggc
     7561 ccgccacgcc tccgccgcca ccggccttcc ggcggtcgcc gacgactccg gcctgtgcgt
     7621 cgaggcgctc ggcggcgcgc cgggggtcat ctccgcccgc ttcgccggcg aacccagatc
     7681 cgacgcgcgc aacaacgccc tgctgctgga aaagctcgcc cacctcccag agcccgcaca
     7741 gcgccgcgcc tatttctatt cggccgtggt gctggtgcgc catgccgaag acccgcgccc
     7801 gctgatcgcc gacggcgaat ggcacggcga gatcctcccc gccgcgcgcg gcgaaggcgg
     7861 cttcggctac gacccgctgt tctgggttcc cgaactggag cagaccgccg ccgagctgga
     7921 ttcggcgctc aagaacaccc tcagccaccg cggcgccgcg atgcgccacc tgctcgaccg
     7981 cctcgccgag caccctcttt aagctgcagc gcagccgcaa cggaacagat tccgccgtga
     8041 ccacccgccg catcatcccg ctgacgccgg ccaccggggc tgcgcgcgcc ctgcccctgc
     8101 ccgaacgccc gcagctcgag gccccgccgc cgctctccct gtacgtgcat ttcccgtggt
     8161 gcgtgaagaa gtgcccctac tgcgacttca actcccacgc cccgcgcgag ggcggcatcc
     8221 ccgagcaggc ctggctagcc gcagtgctcg ccgacctgca gcacgccctg ccccaggtct
     8281 ggggccggcg ggtgcacagc gtcttcatcg gcggcggcac gcccagcctg atgtccgcgg
     8341 ccacgctcga cggcctgctc accggcatcc gcatgctgct gccgctcgac ccgctcgccg
     8401 agatcacgct cgaggccaac ccgggcacgg tcgaggccgg acgcttccgc gactaccgcg
     8461 ccgccggcgt caatcgcctg tcgctcggca tccagagctt cgacgacgcc atgctggcga
     8521 agatcggccg catccacggc ggcgaggagg cgcggcgcgc gatcgaggcc gcgcgcagcc
     8581 acttcgagcg cgtaaacctc gacctcatgt atgccctgcc cggccagacc ctggacatgg
     8641 cactcgccga cctggaaacc gcgatcggct tcggcgtgca gcacctgtcg tgctaccacc
     8701 tcaccctcga acccaacacc cccttcgccc acaacccgcc gccgctgccc gacgacgaca
     8761 ccgccgccga catgcaggag gccatcgagg cccgcctcgc cgccgccggc ttcacgcact
     8821 acgagacctc cgccttcgcc cgcccgcacg agcagagcag gcacaacctc aactactgga
     8881 ccttcggcga ctacctcggc ctcggcccgg gcgcgcacgg caagctctcc agccacgagg
     8941 gcatccgccg cgagatgcgc cacaagcacc cggggcgtta cctcgaaggc gcggcgcgcg
     9001 gcgacttcat ccaggaagcg cgcgaggtgt cggtggccga gctgcccttc gagttcatga
     9061 tgaa
//
LOCUS       DAOQNN010000095         9024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3811_length_9024_cov_10.595942, whole genome shotgun sequence.
ACCESSION   DAOQNN010000095 DAOQNN010000000
VERSION     DAOQNN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3811_length_9024_cov_10.595942"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1405)
                     /locus_tag="PKV97_07080"
     CDS             complement(5..1405)
                     /locus_tag="PKV97_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNB05677.1"
                     /translation="MPPDPRPLPELVSFLETFPEPHILCDRDYRILAANAAYRRSWPE
                     ARSVIGRTCYDVSHHYSVPCDRAGESCPLARSLQSGQRERVLHLHHTPRGEEYVDIEL
                     SPVRDAAGEIAWFIEKMEPLHVARGVSDRRGLIGRSPAFQRMLELIARVAPSDASVLL
                     QGESGTGKELLASAVHEASRRAEGPFVVVDCSGLPETLFESEVFGHERGAFTGATARK
                     PGLVEAASGGTLFLDEVGDIPLAMQVKLLRLLETGTYRRVGSTELRRADIRLVSATHR
                     PLKRMIAEGGFRQDLYFRINTFPITVPPLREREGDLPLLIDSLLERVAPKRKLSLSPA
                     ALRVLSNYAFPGNVRELRNVLERASLMCDGELIGPEHLPEEVLHPESESAESRFASGG
                     PLAAAIPVRDPLDLEEVQRQAMLRAVRAHRGSRRELARRLGISERTLYRRLKSLGLLE
                     GQAMRGGDEEGGGAAR"
     gene            1701..2141
                     /locus_tag="PKV97_07085"
     CDS             1701..2141
                     /locus_tag="PKV97_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNB05678.1"
                     /translation="MSLLAQLKKDSLLARKAADSVRATLLSTLIAEAEMVGKNAGNRE
                     STDDEVQQTIRKFLKNNQEAAAVIRDAGRLAVLERECAVLTAYLPPMATEAEVKAFIA
                     DAVASLAERSPKQMGAVMAALKAKYGSGFDAKQANAWVKEALAA"
     gene            complement(2277..2825)
                     /locus_tag="PKV97_07090"
     CDS             complement(2277..2825)
                     /locus_tag="PKV97_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNB05679.1"
                     /translation="MTKIQTPEQFAAVGQANLEAMMSLANSAIARAERLAELNLNTVR
                     SVLEDSVATTRKLAEAKDPQQLAKLQGELTKPMLDKAVAYARSVQEIATEGQQEVSNL
                     FEKQIADLNKRFHDMLEQAAKQAPAGSEGVFEAVRSAMNTANSLYDNASKAARQAAEV
                     AEANMAAATEATVKAVSAATKK"
     gene            complement(3128..3340)
                     /locus_tag="PKV97_07095"
     CDS             complement(3128..3340)
                     /locus_tag="PKV97_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HNB05680.1"
                     /translation="MAKKFPLHPKNPERICWGCDKYCPVDALGCGNGSGRTQHPIELL
                     GDDWYTYGDWGIDPEDPATPGKKKAA"
     gene            complement(3433..3783)
                     /locus_tag="PKV97_07100"
     CDS             complement(3433..3783)
                     /locus_tag="PKV97_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB05681.1"
                     /translation="MTDRTNAASGETRVEKLKSMLDFQHQAIVSRMLVKNAAGSVTLF
                     AFDKGEGLSEHTAPFDALLIGVEGEAEVRVDADIHRLGEGETLLIPAGAPHAVAPRSQ
                     FKMLLVMLKGERKA"
     gene            complement(3860..4966)
                     /locus_tag="PKV97_07105"
     CDS             complement(3860..4966)
                     /locus_tag="PKV97_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HNB05682.1"
                     /translation="MSLARLFSLPAALSAGRREMALGTLGVAVGLGCTEWIGFLALGG
                     QEPWFILPMGASAVLLFCVPASPLAQPWPSLVGNLVSALIGVGCYRWLGETGTAVALA
                     GCLAVGAMFLLRCLHPPGGAVALTAVLGGPMVHELGYAFALMPVLVNTLAMLVVAFLF
                     NNLVGRRYPHLAPARAQAHGTADPLPSKRVGFRAEDLDAALASFGEVLDVDRDDLEEI
                     MVRAQMNARRRTWGALRCADIMSRDVVSVGPQAPVGEAWALLAHHRIKALPVVVEGGR
                     LVGIVSVPDFFIDRHNPEPQPVPRMRTARVVAEIMSGRVHSARPGQSLADLVGAFSDG
                     GLHHLPVADEDGRLVGMITQSDVVAALFAGEREA"
     gene            complement(5337..5412)
                     /locus_tag="PKV97_07110"
     tRNA            complement(5337..5412)
                     /locus_tag="PKV97_07110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5377..5379),aa:Val,seq:tac)
     gene            complement(5452..5724)
                     /locus_tag="PKV97_07115"
     CDS             complement(5452..5724)
                     /locus_tag="PKV97_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNB05683.1"
                     /translation="MNKQELVASIAEKAGMTKADAARALDATVAAVTEALRQDETVTL
                     VGFGSFYVGERAARTGRNPRTGKNIKIKAAKVPKFRAGKGLKDSVN"
     gene            complement(5943..6527)
                     /locus_tag="PKV97_07120"
     CDS             complement(5943..6527)
                     /locus_tag="PKV97_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5610 domain-containing protein"
                     /protein_id="HNB05684.1"
                     /translation="MNTVLTSASVPAGASVQAADAARQNSAAASRLQRQAPTTESELR
                     KDLHIGILQASIQVSIRSGDQSQALLLRSAIEGINEALAPTLGPQAIQNSMDQDRSAE
                     ATAGRIVSMSTAFFEAYAARRQDDAPETVLRDFIDVIRGGFESGFGEAREILEGLGVF
                     KGAVEADVMKTRELVLEGYDRFIQERLPAAAEPQ"
     gene            complement(6672..6923)
                     /locus_tag="PKV97_07125"
     CDS             complement(6672..6923)
                     /locus_tag="PKV97_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05685.1"
                     /translation="MQAPHRRLTRFVTSALAIWFGARRAWNDMVNTGIVFFMLFRYTK
                     LFDRWWDIMPKWLFFLVVALAAILVLRVLRRPCGRGLPA"
     gene            complement(7176..7985)
                     /locus_tag="PKV97_07130"
     CDS             complement(7176..7985)
                     /locus_tag="PKV97_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB05686.1"
                     /translation="MNPRRLPSRIVCLTTETVEVLYALGEEDRIAGISGYTVIPPRAR
                     KEKPKVFAFSSGDLEKILAVAPDLVLGFSDMQADVARDLIKAGVAVHVFNQRSVEGIL
                     AMVETVGRLVGAEARALTLVAELEATMDAVRARAAALPRRPRVYFEEWDEPPISAVGW
                     VSELIGIAGGEDIFAERARHPGAAQRILADPLEAARAAPDIVVGSWCGKRFRPERVAA
                     RAGWEDVPAVRDDELHEVKSALILAPGPVAIREGLPALAEIVARWAARLHA"
     gene            8291..8686
                     /locus_tag="PKV97_07135"
     CDS             8291..8686
                     /locus_tag="PKV97_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HNB05687.1"
                     /translation="MSAIAEREVNASEDRGALARMVMTLLDHWNLSTEDQAALLGIAA
                     SNRAALSNYRSGKPIGTSRDQYDRVGHLLGIHKNLRLLFPHNRDLAYRWMSTRNKAFD
                     NLSPVEVVKEWGFAGLLMVRGYLDHARGV"
     gene            8699..>9024
                     /locus_tag="PKV97_07140"
     CDS             8699..>9024
                     /locus_tag="PKV97_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HNB05688.1"
                     /translation="MNSPFAQVSLADVHQDVARNIVSLRQSQDLFDDLTDDPAAWLLA
                     QKVEAEVRPPSYRSHTPIIDRPFEDAAWFNAILWPFKHWQASRFSDGSFGVWYGSDSV
                     ETTVFES"
BASE COUNT         1464 a         3149 c         2996 g         1415 t
ORIGIN      
        1 ccactcagcg cgccgccccc ccgccctcct catccccgcc gcgcatcgcc tggccctcga
       61 gcaggccgag tgacttcagc cgccgataca gcgtgcgctc gctgatcccc agccggcgag
      121 ccagctcgcg ccgactgcca cgatgcgcgc gcacggcgcg caacatggcc tggcgctgca
      181 cctcctcgag atccagcgga tcgcgcaccg ggatcgctgc ggccaacgga cctccactgg
      241 caaatcgtga ctcggcgctc tcgctctcgg gatgcagcac ctcctcgggc agatgctcgg
      301 gcccgatcag ctcgccgtcg cacatcagac tggcacgctc gagcacgttg cgcagctcgc
      361 gcacgttccc cgggaaggcg tagttgctca gcacgcgcag cgcggcgggg ctcagcgaca
      421 gcttgcgctt cggcgcgaca cgttcgagca gggagtcgat cagcagcggc aggtcgccct
      481 cacgctcgcg cagcggcggt acggtgatgg ggaaagtgtt gatgcggaag tacaggtcct
      541 gacggaagcc gccctcggcg atcatgcgct tgagcggacg gtgggtggcc gagaccaggc
      601 ggatgtcggc acgacgcagc tcggtggagc cgacgcggcg gtaggtgccg gtctcgagca
      661 ggcgcagcag cttgacctgc attgcgagcg gaatgtcgcc gacctcgtcg aggaagaggg
      721 tgccgccgct ggccgcctcg accagcccgg gcttgcgcgc ggtcgcaccg gtaaaggcac
      781 cgcgctcgtg accgaagacc tcgctctcga acagggtctc gggcaggccg gaacaatcca
      841 ccaccacgaa gggcccctct gcgcgccggc tcgcttcgtg caccgcgctc gcgagcagct
      901 ccttgccggt gccggattcg ccctgcagca acacgctcgc gtccgagggc gcgacccgcg
      961 cgatcagctc gagcatgcgc tggaaggccg gcgaacgtcc gatcaggccg cggcggtcgg
     1021 acaccccgcg ggcgacgtgc agcggctcca tcttctcgat gaaccaggcg atctcgccag
     1081 cggcgtcgcg cacgggcgag agttcgatgt caacgtactc ctcgccgcgc ggcgtgtgat
     1141 gcaggtgcag cacgcgctcg cgctgtcccg actgcaggct gcgcgcgagc gggcaggatt
     1201 ctccggcgcg atcgcagggc acgctgtagt ggtgcgacac gtcgtagcag gtgcgcccga
     1261 tcacgctgcg cgcctccggc cacgagcgcc ggtaggcggc gttggcggcg aggatgcggt
     1321 agtcgcgatc gcacaggatg tgcggttcgg ggaaggtctc gaggaaggag accagctcgg
     1381 gcagcgggcg ggggtcgggc ggcatgtcct gtcagatctg gcggaaggtc tgccagcagt
     1441 ctgcgtcccc gggggcggcg ccgtcaatcg cctcccctgg agccgggcct gggcagcgcg
     1501 agcgggcggc gcgactgcgg caggcgtgct tcggcaacgt gcgccgcgtc agcgggaacc
     1561 gcaggctgcg aggcgcgatc cgggcgctgt ggcggtacgg gctgcggtag actcggcccc
     1621 cgaccggcgt cctgcacgcc gctccgtgac cggaggcctg cgtcgcacgc ctcccccgct
     1681 cgattccacc ggagaccccg atgagcctgc tggcccaact aaagaaagac tccctgctcg
     1741 cgcgcaaggc tgccgacagc gtgcgcgcca ccctgctgtc caccctgatc gccgaggccg
     1801 agatggtcgg caagaacgcc ggcaaccgcg agagcaccga cgacgaggtg cagcagacga
     1861 tccgcaagtt cctcaagaac aaccaggaag ccgcggccgt gatcagggac gccggccgcc
     1921 tggccgtgct cgagcgcgag tgcgcggtgc tgacggccta cctgccgccg atggccaccg
     1981 aggccgaggt gaaggccttc atcgccgacg cggtcgccag cctggccgag cgctcgccca
     2041 agcagatggg cgcggtgatg gctgcgctga aggccaaata cggcagcggc ttcgacgcca
     2101 agcaggcgaa cgcctgggtc aaggaagccc tggccgcttg aggcccgtcg gcaggcccgg
     2161 ccggccactg cggcttccat gcggcgagcc gggcaccatg taagaaaggc cgcccggcaa
     2221 tgccgggcgg ccttttctgt tgcgtggaga agcgaccgcc tctccgcgcg ctgcgatcac
     2281 ttcttggtcg cggcgctcac ggccttgacg gtggcttcgg tcgcggcggc catgttggct
     2341 tcggcgactt cggcggcttg gcgggcggcc ttgctggcgt tgtcgtacag gctgttggcg
     2401 gtgttcatcg ccgagcggac ggcctcgaac acgccttccg aaccggcggg cgcctgcttg
     2461 gcggcctgct cgagcatgtc gtggaagcgc ttgttgaggt cggcgatctg cttctcgaac
     2521 aggttcgaca cttcctgctg gccctcggtg gcgatctcct gaacgctacg ggcgtaggcg
     2581 acggccttgt cgagcatcgg cttggtcagc tcgccctgga gcttcgcgag ctgctgcggg
     2641 tccttggctt cggccagctt gcgggtggtg gcgacgctgt cctcgagcac cgaacgcacg
     2701 gtgttgaggt tgagttcggc caggcgctcg gcacgggcga tggcgctgtt ggcgagcgac
     2761 atcatggctt cgaggttggc ttggccgacg gcggcgaact gctcgggggt ctggatcttg
     2821 gtcatggctg gcttccttgg tgagttgcag tggagtcgat gatgcgaaag aaactcgcgg
     2881 tccatgcggc cttgttgccc gaaaccattc cggcttcgtt ttgctgcacc gcacaatgga
     2941 gccgattata gcgttgggaa ttccaccgtc aacacttttt tgttgcaccg caacatcgtg
     3001 ctgttccacc cgttagccaa gtggtggaaa tcccgctcgc aatcgcattc gccgcgatcc
     3061 tcctgatcct cttccatcga ggtttcaggc gcatgaaaga gccagcgcag cgccctcgcc
     3121 cacctgctca cgccgccttc ttcttgcccg gcgtcgcggg atcctccggg tcgatgcccc
     3181 agtcgccata ggtgtaccag tcgtcgccga gcagctcgat cgggtgctgg gtgcgtcccg
     3241 agccgttgcc gcagccgagc gcatcgaccg gacaatattt gtcgcaaccc caacagatgc
     3301 gctccgggtt cttcggatga aggggaaatt tcttcgccat gaccacactc tccaatacgc
     3361 tgcccgggtt ccgggcgcgc tcgactgccc gcgcgggcac ccggcaggcg gccgggtgcg
     3421 gcacctcccc gctcaggcct tgcgctcgcc cttgagcatc accagcaaca tcttgaactg
     3481 cgagcgcggc gccaccgcat gcggcgcgcc ggcggggatc agcaacgttt cgccctcgcc
     3541 gagccgatga atgtcggcgt cgacgcgcac ttccgcttcg ccctccacac cgatcagcag
     3601 cgcatcgaag ggcgcggtgt gctcgctgag gccctctccc ttgtcgaagg cgaacagcgt
     3661 gacgctgccg gcggcattct tcaccagcat gcggctcacg atcgcctggt gctggaagtc
     3721 gagcatcgac ttgagcttct cgacgcgggt ttcgccgctc gcggcattcg tgcgatccgt
     3781 catttcgggt tcctcccttc tgtcaggcat tccattgcaa tcctactcct gcgcgcgcgg
     3841 gtcgatccgc cggcgtccac taagcctcgc gctcgccggc gaagagcgcc gccaccacgt
     3901 ccgactgggt gatcatgccg accagcctgc cgtcctcgtc ggcaaccggc aggtgatgca
     3961 ggccgccgtc cgagaaggcc cccaccaggt cggcgagcga ttgcccgggg cgggcgctgt
     4021 gcacgcgccc gctcatgatc tcggcgacca cgcgggcggt gcgcatgcgc ggcaccggct
     4081 gcggctcggg gttgtggcga tcgatgaaga agtccggcac cgatacgatg ccgaccagcc
     4141 ggccgccctc caccaccacc ggcaaggcct tgatgcggtg gtgcgcgagc agcgcccagg
     4201 cctcgcccac cggggcctgc ggacccacgc tcaccacgtc gcgcgacatg atgtcggcgc
     4261 agcgcagcgc gccccaggtg cgccggcgcg cgttcatctg cgcgcgcacc atgatctcct
     4321 cgaggtcgtc gcgatcgacg tcgagcacct cgccgaaaga ggccagcgcc gcgtcgaggt
     4381 cctccgcacg aaagccgacg cgcttgctcg gcagcggatc ggccgtgccg tgcgcctgcg
     4441 cacgcgcggg cgcaaggtgc ggatagcgcc gtcccacgag gttgttgaac aggaaggcga
     4501 ccaccagcat cgccagcgta ttgacgagca cgggcatcag cgcgaaggcg tagccgagtt
     4561 catgcaccat cggaccaccc agcaccgccg tcagcgccac cgctccgccc ggcggatgca
     4621 ggcagcgcag caggaacatc gcccccaccg ccaggcagcc ggcgagcgcg acggccgtcc
     4681 cggtctcgcc cagccagcga tagcagccta ccccgatcag cgccgacacc aggttgccga
     4741 cgaggctcgg ccagggctgc gcgagcggac tcgccgggac acagaacagc agcaccgccg
     4801 acgcccccat cgggaggatg aaccacggtt cctgcccgcc cagcgcgagg aagccgatcc
     4861 actcggtgca acccagccct accgccaccc ccagcgtccc cagtgccatc tcgcgccgcc
     4921 ccgcagacaa ggcggcaggc agggagaaca gtcgggcgag ggacatcggc aggctccagc
     4981 ggcgaggcgc aacgaggggt gcccaccggt cggtcaggca ggcggcagac tcccgtctgc
     5041 actgtccggc aagcacggca ccgtgcgggt gggcgctcgc ccttgcggca tcgaaccgca
     5101 tccgcacgag ccgatcatta catcaaaaag tgatatttta aaccgtatct tggattcggc
     5161 gaggactcgc ggcattacaa aatgtatggg tgcgagggtg cgggagtcag cgggaacgga
     5221 tggaggatgg gcggcgggtg cgcaacggag ggcgcgcgac cattcgatca tccacgacaa
     5281 aaaggcccgg tacgcggtcc gggcctttcg tttcgccgag aaatcggcgc attcggtggt
     5341 gggtgctgac ggggtcgaac cgccgacatt cgccttgtaa gggcgacgct ctaccgactg
     5401 agctaagcac ccgcgctttc cccggcgcgg atgccggagc agcaaagtcc gctagtttac
     5461 agaatccttg agccctttgc cagcgcggaa tttcggcacc ttggccgcct tgatcttgat
     5521 gttcttgccg gtgcgcgggt tgcgcccggt gcgcgcggcg cgctcgccca cgtagaagga
     5581 gccgaagccc accagggtga ccgtttcgtc ctgacggagg gcctcggtca ccgcggcaac
     5641 ggtcgcgtcc agcgcgcgcg ccgcatctgc cttggtcatg ccagccttct ccgcgatgct
     5701 tgcaaccagt tcctgtttat tcacttgacc ccctccggtt ttgtactgca atgggtggaa
     5761 ccgtggcttt ggaatgatcg gagcgcacca cgggttgcga catgacctgc ggaactcgcg
     5821 attttatagc gcgcgcgatt cagcactgtc aacgcggcag gaagtgccgc cgtgggacat
     5881 tcgacgagac ttccggcgcg tgcggtgcag catggccgga gcctgcatct ccaccagacc
     5941 acctactgcg gctcagccgc agcgggcagg cgctcttgga tgaagcggtc gtacccctcc
     6001 aacaccagct cgcgcgtctt catcacgtcg gcctcgaccg cgcccttgaa gacgcccaag
     6061 ccttcgagga tctcgcgcgc ctcgccgaag ccgctctcga aaccgccacg gatcacgtca
     6121 atgaagtcgc gcagcacggt ctcgggggca tcgtcctggc gccgcgcggc ataggcctcg
     6181 aagaacgcgg tcgacatcga cacgatgcgc ccggcggtgg cttcggccga acgatcctgg
     6241 tccatggagt tctggatggc ctgcggaccc agcgtgggtg caagcgcctc gttgatgccc
     6301 tcgattgccg agcgcaacaa cagcgcttgt gattgatctc ccgagcggat cgacacctgt
     6361 atcgaagcct gcaggatgcc gatatggaga tccttgcgca actcggactc ggtggttggc
     6421 gcctgacgct gcaggcgcga cgccgccgca ctgttctggc gcgcggcatc cgccgcctgg
     6481 acggacgcac ccgcggggac ggaagcactc gtcaggacgg tattcatggc ggactccttt
     6541 ggcgttgttc tctcacttcc agtctagaca gcggcccggc atcgcgctgc gccaagaccg
     6601 ctcaaacgtc gcgagtccgc agccgcccgg gatgatcatc gccccgacgg cgagcgtgag
     6661 cgcgcgcgga ctcatgccgg cagcccccgt ccgcacgggc gccgcagcac ccgcaggacc
     6721 aggatcgccg ccagcgcgac gacgaggaag aacagccact tcggcatgat gtcccaccac
     6781 ctgtcgaaga gcttggtgta tcggaagagc atgaagaaca cgattccggt gttgaccatg
     6841 tcgttccacg cgcggcgcgc gccgaaccag atcgccaggg cgctggtgac gaagcgtgtg
     6901 aggcgccggt gcggggcttg caccgatcag gatcgccacc tgcaccgtct cgggaaagaa
     6961 gccccggaac aggcgggaga ggaagaacac gctcgccaac atcgcgagcg caccgagaaa
     7021 ggacgccacc cgcatgccga gcgagacatg gcgaacgcac tgcctcgtcg gccggtctcg
     7081 atgacgtggg cgaattcggc gccacgctgc ccggcaaggc gtgtgcggac gactccggta
     7141 ccggactgcc cgccgcgctg cgcgaccgcg ccgcttcagg cgtgcagccg agccgcccag
     7201 cgcgcgacga tctccgccag cgccggcagc ccctcgcgga tcgccaccgg ccccggcgcc
     7261 aggatcagcg cggatttcac ctcgtgcagc tcgtcgtcgc gcacggccgg aacgtcctcc
     7321 caccccgcac gtgcagccac acgctcgggg cggaagcgct tgccgcacca cgagcccacc
     7381 acgatatccg gcgccgcccg cgccgcctcc agcggatcgg cgaggatgcg ctgcgccgcg
     7441 cccggatggc gcgcgcgctc ggcaaagatg tcctcaccgc cggcgatccc gatcagctcc
     7501 gacacccagc ccaccgcgct gatcggcggc tcgtcccact cctcgaaata cacccgcggc
     7561 cgccgcggca gcgccgccgc ccgcgcgcgt accgcgtcca tcgtcgcctc gagctcggcc
     7621 accagggtca gcgcacgcgc ctccgcccca accagccggc ccaccgtctc gaccatcgcc
     7681 aggatgccct cgacgctgcg ctggttgaac acatgcaccg ccaccccggc cttgatcagg
     7741 tcacgcgcca cgtccgcctg catgtcggag aaccccagca ccaggtccgg cgcgaccgcg
     7801 aggatcttct ccaggtcgcc gctggagaag gcgaacacct tcggcttctc cttgcgcgca
     7861 cgcggcggga tgactgtgta gccgctgatg ccggcgatac ggtcctcctc gcccagggcg
     7921 tagaggactt cgacggtctc ggtagtcaga cagacgatgc gggaggggag gcggcgtggg
     7981 ttcatggcgg gattatcgcg catcccctcg ggcacgcgtg ctcagccccg gtctgctcct
     8041 cgcccatccg ccgcgcgtgc ggcctgcatg ctgggagcga cccgcttcgc gatccccacg
     8101 tgcagaaacc ggtgcgatcc ggaatcgtcg gctttggcga ccgattgcga acggcctttc
     8161 ggctacctgg aggacgctcg acttgaaacg gcggcctggc ttcaggcgtt acctttaaca
     8221 ctcctcttgt tttctttaaa acagacaact atcctcgaag taccgatcac caccaagcga
     8281 ggagcccgcc atgagcgcca tcgccgagcg cgaagtgaac gcatcggaag accgcggtgc
     8341 gctggccagg atggtcatga ccctgctcga tcactggaac ctgagcaccg aggaccaggc
     8401 cgcgctgctc ggcatcgccg ccagcaaccg ggcggcgctg tcgaactacc gcagcggcaa
     8461 gccgatcggc accagccgcg atcagtacga ccgggtgggg cacctgctcg gcatccacaa
     8521 gaatctgcgc ctgctgtttc cgcacaaccg ggacctggcc tatcgctgga tgagcacccg
     8581 caacaaggcc ttcgacaacc tgagcccggt cgaggtggtc aaggaatggg gctttgccgg
     8641 gctgctgatg gtgcggggct acctcgacca cgctcgtggc gtgtgagccg ggcgcacgat
     8701 gaattcgccg ttcgcgcagg tctcgctggc cgacgtccat caggacgtgg cgcgcaatat
     8761 cgtgtcgctg cgccagtcgc aggatctgtt cgacgacctg acggacgatc cggccgcgtg
     8821 gctgctggcg cagaaggtgg aggcggaggt caggccgccg tcgtatcggt cgcacacgcc
     8881 gatcatcgac cggcctttcg aggatgccgc ctggttcaac gccatcctgt ggccgttcaa
     8941 gcattggcag gcgagccggt tctccgacgg atccttcggc gtgtggtacg gctcggactc
     9001 ggtcgagacc acggtcttcg agtc
//
LOCUS       DAOQNN010000096         8998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3827_length_8998_cov_7.958403, whole genome shotgun sequence.
ACCESSION   DAOQNN010000096 DAOQNN010000000
VERSION     DAOQNN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8998
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3827_length_8998_cov_7.958403"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..499
                     /locus_tag="PKV97_07145"
     CDS             287..499
                     /locus_tag="PKV97_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05689.1"
                     /translation="MKTSIVMFIALTGITASSAAFAGRDSFQIWQHERFVKQKQAQEA
                     ELARLRMCAAAQQAASTTGTASAAQR"
     gene            complement(504..956)
                     /gene="cadR"
                     /locus_tag="PKV97_07150"
     CDS             complement(504..956)
                     /gene="cadR"
                     /locus_tag="PKV97_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HNB05690.1"
                     /translation="MRIGELARATGVDIETIRYYEKGGLLAPPARMPNGYRAYSAAHL
                     ERLAFIRHCRALDMPLAEIRRLLDFVAHPEADCAGINRLIDAQLTRVRARLKSMRALE
                     KQLAALRVRCEAGHVAAECGILHELVAAAHGEACACHAPGEVAGAKKR"
     gene            1021..3222
                     /locus_tag="PKV97_07155"
     CDS             1021..3222
                     /locus_tag="PKV97_07155"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNB05691.1"
                     /translation="MAVMNAPEPALKQSVFVVRNMDCPTEEALIRKRLGALAGVAELA
                     FDLMARRLTVRHTLADEQPILGALREIGMDANAPRDAGHACCGGACTATAAAVEAPAE
                     AHAEPPIVSHRTQLLMAVSGVAALAAEGLAWTGADESSPLVIALALLSIAAGGLGTLK
                     KGWIALKTFTLNINFLMSVAVIGAVAIGEWPEAAVVIFLFALAELIETLSLERARNAI
                     RGLMAIAPETATVRLGDGAWETRPSARVQIGDTVRVKPGERIPLDGVVTAGASAVNQA
                     PITGESIPVDKTPGDPVFAGTVNERGAFEYRVTANKGNTTLARIIHAVQEAQGQRAPT
                     HRFVDRFARYYTPAVVAVALLVATLPPLLAGAAFTEWFYTALVLLVIACPCALVISTP
                     VTVVSGLAAAARHGILVKGGVHLENGRLLRAVAFDKTGTLTHGRPVLTDVIPLVERPT
                     DELLELAASIDAHSEHPVAAAIVAGWQARAQAALPLPDVTGFEALAGRGAKGMLEGLT
                     YYVGNHRLIEDLALSGPQVEDALLRIERAGKTAVVLATQHAPLCVFGVADTLRGHSAE
                     AIRALHALGVVSVMLTGDNPMTARAIADQAGIDDARGNLLPEDKLAAIDALVARYGHA
                     GMVGDGINDAPALARASIGFAMGAAGTDTAIETADVALMDDDLRKLPRFIALSRRTAQ
                     VLWQNIAFALGIKAVFFALALAGQATLWMAVFADMGASLLVVANGLRLLRR"
     gene            3299..4360
                     /locus_tag="PKV97_07160"
     CDS             3299..4360
                     /locus_tag="PKV97_07160"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNB05692.1"
                     /translation="MTNALLKSAGLVSIAATLAAAAPALAAGTVYVTNQGGGVTLLDP
                     VKLAPEQTIELKDNDPRGLAVTPDGKYLLTANQKTGDVAVIDTATRKVVRRVPIGKNP
                     EFLRILPDGAKAFVTYEPGSEGGPPKKGGKDDDDDGPKIPGQVAVIDLKSWKVVASIV
                     GAPETEGIEFSRDNKRVAISNEGNDTVTVYDIGTGKLQKTVDLHAQGSRPRGIKLAPD
                     GSRYVVTMENSNGMVILDPEFNVVKIVPTGKGPYGVAFDKTGKHIVVAASRDNLLQVF
                     DGTSYAKLAEVPVGKRCWHFSYTPDESQLLVACGRSNEVQVIDATTWQPVKAIAGFDL
                     PWGIVTYPKADGSLDAPAK"
     gene            4409..5254
                     /locus_tag="PKV97_07165"
     CDS             4409..5254
                     /locus_tag="PKV97_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNB05693.1"
                     /translation="MSNKRRQFLKALSALPLAAQLPARADEPLDAIRKSGRLSIAVYD
                     NFPPYSQAGKGIDVDLGKALAEKLGLVPVIVEFKAGENMSDDLRNMVWKGHYLRGEPA
                     DMMVHVPVDPVLAEANDKVRIFGTYHYETIAMARMASRVPAPIGSAAVALEVFTREKI
                     GVEGDTLADSFMLGASRGRFRENVVHFRSVEQAVAALKEGEVSAVLAPRAEIEAALRG
                     ETRFAVDEAKLADLRNGRWPLGMAVKAEAADLAAALTRALAELQQAGTVAAIFKRYGV
                     TAQAT"
     gene            5304..5450
                     /locus_tag="PKV97_07170"
     CDS             5304..5450
                     /locus_tag="PKV97_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05694.1"
                     /translation="MSLVAPSLAFAGAIDRARGPGNTVDAVFADMGASLLVVANGLRL
                     LRER"
     gene            5531..6112
                     /locus_tag="PKV97_07175"
     CDS             5531..6112
                     /locus_tag="PKV97_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNB05695.1"
                     /translation="MAPQTDAASQEEPARYPRTAVLVHWTVALLILALLASGWYMVGT
                     PKNTPERAFWFNLHKSLGIVAAMGIAALIVWRIRHAAPALPATMARWERIAAALNHRL
                     FYVFMALVTVAGYLTSSFSKYGRKLFGLPMPYWGWEDLALREELATVHRVAALVFAVL
                     IAIHIAAALKHLLVDRDGVFQRMLPDRAKGAKQ"
     gene            6109..6348
                     /locus_tag="PKV97_07180"
     CDS             6109..6348
                     /locus_tag="PKV97_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNB05696.1"
                     /translation="MMWEYGVGWHWLGWLGMALFWLIPVLLVLVAVKYLGSKPTPPPG
                     SQTGKTALDYLNEAYAKGEISRDEFLQKRADLKGQ"
     gene            6442..6756
                     /locus_tag="PKV97_07185"
     CDS             6442..6756
                     /locus_tag="PKV97_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05697.1"
                     /translation="MSLQSSWAMAMSYCQHETGQAAQHFAHHGHQHQQTSPDGEDGAA
                     SSTPFQPHADCGACHLNCPAVASPSCVPGVVRSAFVVADPPQTYLSVFPEGPERPNWL
                     PA"
     gene            6920..7585
                     /locus_tag="PKV97_07190"
     CDS             6920..7585
                     /locus_tag="PKV97_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05698.1"
                     /translation="MKRILLSSALLFALAGCASVSDLPRDYTPGAANSEGLAVVSLTL
                     SGKDLGSVASFEYRVRDAAAQFDADVRRRPYFDSAKQQARWLLDRDAQGPTAARMRVI
                     VKDQASAEPLDVVEAGKAIGRLAAMRLPPGDYELYAWKVVEPNRYGGNEFTPKRVFSY
                     PFKVEAGRATYLGNLDLRMSEQDTYKLSVDDKAARDLALLSKKLPALRAEQIVHGVGK
                     VLP"
     gene            7647..8003
                     /locus_tag="PKV97_07195"
     CDS             7647..8003
                     /locus_tag="PKV97_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05699.1"
                     /translation="MKKLLVLLMLFALPLHYSWAAVASYCQHESGQAAQHFGHHAHQH
                     QAGSADGEDGTSGTAPFQAHADCGMCHMSCPAVAVPLHSIGIVIPGSFVVAEPPPPLS
                     SVIPEGPERPKWARLA"
     gene            8137..>8998
                     /locus_tag="PKV97_07200"
     CDS             8137..>8998
                     /locus_tag="PKV97_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB05700.1"
                     /translation="MSLPRLAAALALAACAMAAPAAEPALTLERAWQLAEEANPELRA
                     AQAALAAAEGQLADARGLLWNNPQLAAERDRRRVAQPSASADIQREWRAELSQTLEIA
                     GQHGHRRAAAEQELSAVKENFEETRRQVRAEVERRFVRVLALQTRTATESELVGLIRD
                     AAGAARKRFEAGEDTKLDANLADVELGRAESQLEAAREQSIQARAELAAALQLPAESL
                     PQAQGALGAAAAPPYSLDELQAAAAARPRLRALEHQEQAARSRLGLERAAAYTDVTVG
                     LFAGREGPGEA"
BASE COUNT         1467 a         3047 c         3068 g         1416 t
ORIGIN      
        1 ccgaccctca gccgtcattc gatgctcagc caagcagtcg ttcgtggagc cccggagatt
       61 taggagattc atgagaattt gccgcaccgg cgatgcgttt atccgcagcg ggcgcgcgat
      121 gttgggctcg gcatcgatct cgatggtctt cttgcgggcc aagatgcgtg cgaggggtga
      181 gaccaaattg aagttcgcgt gatacggggg tgattcttcc ggcccacgat tccgatcgtc
      241 gcagcagcga cgcactcgga acccaactct cgtcacggag actcccttga agacatcaat
      301 cgtcatgttc attgccctca ccggcattac cgcatcgagc gccgccttcg caggcaggga
      361 ttcgttccag atctggcagc acgagcgctt cgtcaagcag aagcaggcgc aggaggcgga
      421 actcgcgcgg ctgcgcatgt gcgcggcagc ccaacaggcc gcttcgacca ccggcaccgc
      481 gagcgctgcg cagcgctgag cggtcagcgc tttttcgcgc cggcgacctc ccccggcgca
      541 tgacaggcgc aagcctcgcc atgcgcggcg gccaccagct catgcaggat gccgcactcg
      601 gcggccacat gccccgcttc gcagcgtacg cgcagcgcgg cgagctgctt ctccagcgcg
      661 cgcatcgact tcaggcgcgc gcgcacgcgc gtgagctgcg cgtcgatcag gcggttgatg
      721 ccggcgcagt cggcctcggg atgggcgacg aaatcgagca ggcgccggat ctcggcgagc
      781 ggcatgtcga gcgcgcggca gtggcggatg aaggcgagcc gctcgaggtg cgcggcgctg
      841 taggcgcgat agccattcgg catgcgcgcc ggcggcgcca gcaggccgcc tttctcgtag
      901 tagcggatgg tctcgatgtc cacgccggtg gcgcgggcga gctcgccgat gcgcatgtgg
      961 cctccttttc cgatttcgcc agtgtagccg cttgaccccg gagccgctcc agggtttcca
     1021 atggcggtca tgaacgcgcc agaacctgcc ctcaagcaat cagtgttcgt cgtccgaaac
     1081 atggactgtc ccaccgagga agcgctgatc cgcaagcgcc tgggcgcgct cgcgggtgtc
     1141 gcggagctcg ccttcgacct gatggcgcgt cgcctcacgg tgcgtcatac gctggccgac
     1201 gagcagccta tcctcggcgc gctgcgcgag atcggcatgg acgcgaacgc gccgcgcgat
     1261 gccggacacg cctgctgcgg cggggcctgc accgcgacgg cggcagcggt cgaggcaccg
     1321 gccgaagcgc acgccgagcc gcctatcgtg tcgcaccgca cgcagctgct gatggcggta
     1381 tccggtgtcg ccgcgctggc ggccgaaggc ctcgcctgga cgggcgccga cgagagctcg
     1441 ccgctcgtca tcgcgctcgc gctgctgtcg atcgccgcgg gcggtctcgg cacgctgaag
     1501 aagggctgga tcgcgctcaa aaccttcacg ctcaatatca acttcctgat gagcgtcgcc
     1561 gtgatcggcg cggtcgccat cggcgagtgg ccggaagcgg cggtggtgat cttcctgttc
     1621 gcgctggccg agctgatcga gacgctgtcg ctcgaacggg cccggaacgc gatccgcggg
     1681 ctgatggcga tcgcgcccga gacggcgacg gtgcgcttgg gcgacggcgc gtgggaaacg
     1741 cgtccgtccg cacgcgtgca gatcggcgac acggtgcgcg tcaagcccgg cgagcgcatc
     1801 ccgctcgacg gcgtcgtgac ggccggcgcg agcgcggtga accaggcgcc gatcaccggc
     1861 gagagcatcc cggtcgacaa gacgccgggc gatccggtgt tcgccggcac ggtgaacgag
     1921 cgcggcgcct tcgagtaccg cgtgaccgcc aacaagggca acaccaccct ggcgcgcatc
     1981 atccacgcgg tgcaggaagc gcagggccag cgcgcgccga cgcatcgctt cgtcgatcgc
     2041 ttcgcgcgct actacacgcc ggcggtcgtg gcggtcgcgc tgctggtggc gaccctgccg
     2101 ccgctgctgg ccggcgccgc cttcaccgaa tggttctaca cggcgctggt cctgctagtg
     2161 atcgcctgcc cctgcgcgct ggtgatctcg acgcccgtca ccgttgtcag cgggctcgcg
     2221 gcggcagcgc gccacggcat cctggtcaag ggcggcgtgc atctggaaaa cgggcgactg
     2281 ctgcgggccg tcgccttcga caagaccggc acgctgaccc acggacggcc ggtgctgacc
     2341 gacgtcatcc cgctcgtcga gcgacccacc gacgaattgc tggagctcgc cgcgagcatc
     2401 gatgcgcact ccgagcatcc ggtcgccgcg gccatcgtcg ccggctggca ggcgcgggca
     2461 caggctgcgc ttccgctgcc agatgtgacg ggcttcgagg cgctcgccgg tcgcggcgcg
     2521 aaaggcatgc tcgaggggct cacgtactac gtcggcaacc accggctgat cgaggatctc
     2581 gcgctctccg gcccgcaggt ggaagacgcg ctgttgcgca tcgagcgcgc aggcaagacc
     2641 gccgtcgtgc tcgccaccca gcacgcgccg ctgtgcgtgt tcggcgtcgc cgatacgctg
     2701 cgcggccaca gcgccgaggc gatccgcgcg ctgcacgcgc tgggcgtcgt ctcggtcatg
     2761 ctcaccggcg acaacccgat gaccgcgcgc gcgatcgccg accaggccgg catcgacgac
     2821 gcacgcggca acctgctgcc cgaggacaag ctcgccgcga tcgacgcgct cgtcgcgcgc
     2881 tacggccacg ccggcatggt gggcgacggc atcaacgacg caccggcgct cgcgcgcgcc
     2941 tcgatcggct tcgcgatggg cgcggccggc accgacaccg cgatcgagac cgccgacgtg
     3001 gcgctgatgg acgacgatct gcgcaagctg ccgcgcttca tcgcactctc gcgccgcacc
     3061 gcgcaggtgc tctggcagaa catcgcgttc gccctcggca tcaaggccgt gttcttcgcg
     3121 ctcgcgctcg cggggcaggc gacgctgtgg atggcggtat tcgcggacat gggggcgagc
     3181 ctgctggtgg tggcgaacgg cttgcgactg ctgcgcagat agcgcggcgt cggttgggcc
     3241 gcggctcagg tcgtatctcc gataaggacg taagtcagac acgaagagag gatctcttat
     3301 gacaaatgcc ttgctgaaat ccgccggtct ggtttccatc gcggcgacgc tcgcggcggc
     3361 cgcgcccgcg ctggcggcgg gaaccgtcta cgtcaccaac cagggcggcg gcgtgaccct
     3421 gctggatccg gtgaagctcg cgccggagca gaccatcgag ctcaaggaca acgacccgcg
     3481 cggcctggcg gtcacgcccg atggaaaata cctcctcacc gccaaccaga agaccggcga
     3541 cgtggcggtg atcgacaccg ccactcgcaa ggtggtgcgc cgcgttccca tcggcaagaa
     3601 ccccgagttc ctgcgcatcc tgcccgacgg agccaaggcc ttcgtgacct acgagcccgg
     3661 ttcggagggc ggcccgccga agaagggcgg caaggacgat gacgacgacg ggcccaagat
     3721 tcccggccag gtggcggtca tcgacctcaa gagctggaag gtggtcgcct ccatcgtcgg
     3781 cgcgccggaa accgagggca tcgagttctc cagggacaac aagcgcgtcg ccatttccaa
     3841 cgagggcaac gacacggtaa cggtctatga catcggcacc ggcaagctgc aaaagaccgt
     3901 ggatctgcat gcccaaggct cccggccgcg gggcatcaag ctcgcgcccg acggcagccg
     3961 ctacgtggtc accatggaga attccaacgg gatggtgatc ctggaccccg aattcaacgt
     4021 ggtgaagatc gtgcccaccg gcaaggggcc ctacggcgtg gccttcgaca agacgggcaa
     4081 gcatatcgtc gtcgccgctt cccgcgacaa cctgctgcag gtcttcgacg gcacgagtta
     4141 cgccaagctc gccgaggttc cggtgggcaa gcgctgctgg catttcagct ataccccgga
     4201 cgagagccag ctactggtgg cctgcggacg atccaacgag gtgcaggtga tcgacgcgac
     4261 cacctggcag cccgtcaaag ccattgcagg cttcgatctg ccctggggta tcgtcaccta
     4321 tcccaaggca gacggcagcc tggacgcgcc tgccaagtag gcgccgtccg tggtcgtgcc
     4381 caacgaaatc atcaaggaca gggacaatat gtcgaacaag cgtcgccaat tcttgaaggc
     4441 cctctccgcc ctgcctctgg ccgcgcagct gccggcccgg gcggacgagc cgctcgatgc
     4501 catccgcaag agcgggaggc tcagtatcgc cgtctatgac aatttcccgc cctactcgca
     4561 ggccggcaag ggcatcgacg tggatctcgg caaggccctt gcggaaaagc tgggcctcgt
     4621 gccggtcatc gtcgagttca aggcaggtga gaacatgagt gacgacctgc gcaacatggt
     4681 atggaaggga cactacctgc ggggcgaacc ggccgacatg atggtgcacg tgccggtcga
     4741 tcccgttttg gccgaggcca acgacaaggt acgcatcttc ggtacttacc attacgaaac
     4801 catcgccatg gcgcggatgg cctcccgcgt gccggcgccc atcgggtccg cggcggtggc
     4861 cctggaggtc ttcacccgcg agaagatcgg cgtcgagggc gacaccttgg cggactcctt
     4921 catgctcggc gcgagccgtg gccgcttccg ggagaacgta gtgcatttcc ggtcggtcga
     4981 gcaggccgtc gcggcactca aggaaggcga ggtgtccgcc gtcctggcgc ctcgggcgga
     5041 aatcgaagcg gccttgcggg gcgagacgcg ctttgccgtc gacgaggcca agctcgcgga
     5101 cctcaggaac ggccgctggc ccttgggtat ggcggtcaag gccgaagcgg cggacctcgc
     5161 cgcggcgctc acccgcgccc tagccgagct gcagcaggcc ggtacggtcg ccgcgatctt
     5221 caagcgttac ggggtcactg cgcaggcgac gtaacgggcg gctatgcagc cagcttcggc
     5281 cgcagcgcca agcgcgcagc tcgatgtcgc tcgtcgcgcc cagcttggcc ttcgccggcg
     5341 cgatcgaccg cgcccgcggg ccaggcaaca ctgtggatgc ggtgttcgcc gacatggggg
     5401 cgagcctgct cgtggtcgcg aacgggctgc gcctgctgcg tgagcgctga acggttgaac
     5461 tcaagcccgg gcatcgctgc ccaactggct ggcaccggag gacatggcga tgaaacgcgt
     5521 ggaagcagac atggcgccgc agaccgatgc agcctcgcag gaggaaccgg cgcgctatcc
     5581 gcgcacggcc gtgctggtgc actggaccgt ggcgctgctg atcctcgcct tgctggcgag
     5641 cggctggtac atggtcggca cgccgaagaa cacgcccgag cgtgccttct ggttcaacct
     5701 gcacaagtcg ctcggcatcg ttgcggcgat gggtatcgcg gcgctgatcg tctggcgcat
     5761 ccggcatgcg gcacccgcgc tgcctgccac catggcccgc tgggaacgca tcgcggccgc
     5821 gctcaaccac cgtctgttct acgtcttcat ggcgctggtg acggtcgccg gttatctcac
     5881 ttcgtccttc agcaagtacg gccgcaagct cttcggcctg ccgatgccgt attggggatg
     5941 ggaggatctc gcgctgcgcg aagaacttgc gaccgtccat cgcgtggccg cactcgtctt
     6001 cgccgtgctg atcgcgattc acatcgcggc ggcgctcaag cacttgctgg tggaccggga
     6061 cggggtgttt cagcgcatgc tgccggaccg cgcgaaaggg gcaaagcaat gatgtgggaa
     6121 tacggcgtcg gatggcactg gctgggctgg ctcgggatgg cgttgttctg gctgatcccc
     6181 gtcctgctcg tgctggtggc cgtcaagtac ctgggctcga agcccacgcc gccgcccggt
     6241 tcgcaaaccg gtaagacagc gctggactat ctgaacgagg cttacgccaa aggcgagatt
     6301 tcccgcgacg agttcctgca gaagcgcgcg gatctgaaag gccagtgagc ggcgctatcc
     6361 gttcgcaaag atgatggctc gcgcagcgac tacaattgtg agcgaatgag aaaacttctt
     6421 gcactccttc tgctgtttac gatgtcgctg caatcttcat gggcgatggc gatgtcgtat
     6481 tgccagcacg agaccggcca ggccgcgcag cacttcgctc accacggtca ccagcatcag
     6541 cagacctcgc cggacggcga ggacggtgcc gcatcgtcga cgccattcca gccccatgcc
     6601 gactgcggcg cctgtcatct gaactgcccg gccgtcgcct caccttcctg cgttcccggc
     6661 gtcgtgcgca gcgccttcgt cgtcgccgat ccgccacaga catatctgtc cgtgtttccc
     6721 gagggccccg aacggcccaa ctggctcccg gcttagaccg ccgggaccga cgctctcctc
     6781 gttccaccaa gccgctcgct tcgcacgcgt tgtcgttttg tcgatgacat ctgcggctat
     6841 tccgctgtgc tgttgccggc cgaacgcgag agcagtcgag attgaaatga agccattcat
     6901 cgtgcaggag gtagttgcca tgaaacgaat tctgttgtcg agtgcgttgc ttttcgcgct
     6961 ggcgggctgc gccagcgtgt cggatctgcc gcgcgactac acgccgggag cggcgaattc
     7021 cgagggcctc gcagtggtgt cgctgacgct gtcaggcaag gatctgggca gcgttgccag
     7081 tttcgaatat cgggtgaggg atgcggccgc ccagttcgac gccgacgtga ggagaaggcc
     7141 gtacttcgac tccgccaagc agcaggcaag atggttgctg gacagggacg cgcaaggccc
     7201 gacggccgcg cgcatgcggg tgatcgtcaa ggatcaggcc tcggccgagc cgctcgatgt
     7261 cgtcgaggcc ggcaaggcga tcgggcggct ggcggcgatg cggctgccgc ccggcgacta
     7321 cgagctctat gcatggaagg tggtggagcc caaccggtac ggcggcaacg agttcacgcc
     7381 caagcgggtt ttcagctacc ccttcaaggt cgaagccgga cgcgcgacct acctcggcaa
     7441 tctggatctg cgcatgagcg agcaggatac gtacaagctc tcggtcgacg acaaggcggc
     7501 gcgggatctt gcgctgttgt cgaaaaagct gcctgcgctg agagccgagc agatcgtcca
     7561 tggcgtgggg aaagtgctgc cgtgaaattc gcagccgggt cggcgcgatc gctcgccgac
     7621 ccgccagccg atataatcac cgctcgatga aaaagctgct cgtcctgctc atgctgttcg
     7681 cgctgccgct gcactactcg tgggcggcgg tcgcgtccta ttgccagcac gagagcggcc
     7741 aggcggcgca gcatttcggt caccacgcgc atcagcatca ggcggggtcg gccgacggcg
     7801 aggacggcac gagcggcacc gcgccgttcc aggcgcatgc cgattgcggc atgtgccaca
     7861 tgagctgccc ggccgtggcc gtgccgctcc actcgattgg catcgtcatt cccggatcgt
     7921 tcgtcgtcgc cgagccgccc ccacccttgt cgtccgtcat tcccgagggc cccgagcggc
     7981 ccaagtgggc gcgcctcgcc tgatccggcg ggacgccctc agtcaccagc ttcccggtcg
     8041 ccgcgccgcc tgcatgtgca ggcgggtgcg ttccgtcgga tttctccccc gatccaccgc
     8101 acggagattt cgacggacat gtatccgatc agctatttgt cattgcctcg ccttgccgcg
     8161 gcgctcgcgc tggcggcgtg cgcgatggcc gcgccggcgg ccgagccggc cctgaccctg
     8221 gaacgggcct ggcagctcgc cgaagaggcc aatcccgagc tcagggcggc gcaagccgcg
     8281 ctcgccgccg ccgagggtca gctcgccgat gcgcgcggcc tgctctggaa caacccgcag
     8341 ctcgcggccg agcgcgaccg gcgcagggtc gcccagccga gcgcaagcgc cgatatccag
     8401 cgtgaatggc gcgccgagct ctcgcagacg ctggagatcg ccggccagca cggccatcgc
     8461 cgtgcggcgg ccgagcagga gctgtcggcg gtgaaagaga atttcgagga gacgcgccgg
     8521 caggtgcgtg ccgaggtcga acgacgcttc gtccgcgtgc tcgcgctgca gacgcgcacc
     8581 gcgacggaga gcgagctcgt cggcctgatc cgcgatgcgg cgggcgcggc gcgcaagcgc
     8641 ttcgaggccg gcgaggacac caagctcgac gccaatctcg ccgacgtgga actggggcgc
     8701 gccgaaagcc agctcgaggc ggcgcgcgag cagtcgatcc aggcccgcgc cgagctcgcc
     8761 gcagcgctgc aactgccggc cgagagcctg ccgcaagcac agggggcgct cggcgccgcg
     8821 gcggcaccgc cttactcgct cgacgagctg caggccgcgg ccgctgcgcg cccgcgcctg
     8881 cgcgcgctcg agcatcagga gcaggccgcg cgcagccgcc tcggcctcga gcgcgccgcg
     8941 gcgtacacgg atgtcacggt cggcctgttc gccggccgcg aaggtccggg cgaggcgc
//
LOCUS       DAOQNN010000097         8983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3834_length_8983_cov_7.867944, whole genome shotgun sequence.
ACCESSION   DAOQNN010000097 DAOQNN010000000
VERSION     DAOQNN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3834_length_8983_cov_7.867944"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2069
                     /locus_tag="PKV97_07205"
     CDS             <1..2069
                     /locus_tag="PKV97_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB05701.1"
                     /translation="SFHYRAPDTHMRGTPRPLFLEITFIDRDGHERIKALSETGRQVL
                     DPGLRDITRRENTFVRAETYWPALKALRPGEIHVSRVIGAQVRTHWIGDYTPTRAQAL
                     GKPFAPEDSGYAGLENPVGKRFRGLIRWAAPVLRQDRIIGYVTLALDHAHLMAFTDTL
                     RPTPERFAPIADPASGNYAFIWDDEGHNISHARDYFIHGYDPATGERSVPWLDRELYE
                     AWRSSGQSWPTFSAALTPYQDQRLTREPHPESTASGLVALDCRYLDFSPQCQGWHDMT
                     EHGGSGSFTILFSGLRKLTTVATIPYYTGQYGRTPRGFGYVTIGANVDDFHRAATESG
                     QRIGKMIGEADALTQHERDTLVGDIADQLRGTTTRLAASTLAMVLAVILIAIWMAGLL
                     SRRITHVVDGIHRFEQGDLDQRLAAAGGDELAELARAFNRMADTVQRSIRSLEDARHT
                     AEQANRMKSEFLANMSHELRTPLNGVIGFAELLSMELEDPEQRECATTIHDSARHLLA
                     VVNDVLDLAKVEANRLTLVAQTIDLVPLVASVAALHKVNAEAKGVDLRLDLPAHPCIV
                     DADPVRLRQIIENLLSNAVKFTREGHILVGLAAAADEISIRIADTGCGIARDEQERVF
                     APFYQAASFLNRGHGGTGLGLTLSRRLAHRMGGTIDLDSVPGKGSTFTLRLPRHFTAP
                     AGGPPS"
     gene            2066..2434
                     /locus_tag="PKV97_07210"
     CDS             2066..2434
                     /locus_tag="PKV97_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB05702.1"
                     /translation="MNKLAFIIDDSAVNVAVARLLLRRLGWVVEDFDSAIPMLARLAE
                     VQPAFMLLDISMPDLGGEEACRRIRANAAWNGVRVIAYTAHAMETERQRYLAGGFDAV
                     ITKPITIGGLHAAVGHAPND"
     gene            2449..2829
                     /locus_tag="PKV97_07215"
     CDS             2449..2829
                     /locus_tag="PKV97_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNB05703.1"
                     /translation="MPPHTPESTSHFDLAALRSDMSESRAAVRVVLETFAPWLTDTRQ
                     QLRAAIESAEAEPLGRVAHRLRGALAQLRAAPAVALVKKLEAHCKANPEACLSRDHAV
                     LAELDAELDALAAEVAAQLEALRR"
     gene            2924..3014
                     /locus_tag="PKV97_07220"
     tRNA            2924..3014
                     /locus_tag="PKV97_07220"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2958..2960,aa:Ser,seq:gga)
     gene            complement(3351..4133)
                     /locus_tag="PKV97_07225"
     CDS             complement(3351..4133)
                     /locus_tag="PKV97_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNB05704.1"
                     /translation="MAAYWDEAAMQRALVMPSILAIGDSWFWYPFPGGSLISYLGPIV
                     EKKEHTILAQGMNGAEAFDYVDGKYKDRITGALRRYGSSLSAVFISGGGNDFAGFNDM
                     RPLLALDCSGAQSAGDCFRSGDGGLGQFLERMDDHYRRLIGLIYTHTSLDCLILMHSY
                     DYAIPDGRGVAGKAGWLKPALVDAGVPEALQRDCVRYLIDAFHAMLGRIAMGDPAHLR
                     IVDSRGTLAEEDWANELHPKGKGFRKIAKERWKPVLQGAGLA"
     gene            complement(4335..5768)
                     /locus_tag="PKV97_07230"
     CDS             complement(4335..5768)
                     /locus_tag="PKV97_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNB05705.1"
                     /translation="MRRASLPGCPRSALLPALALLLAACQPAAFVRPDVPVPASWPRQ
                     AEVAQAEAAASLGVQPWREILPAPELHALIEEALAANADLRIAIERIELARAQYGVER
                     AALFPGVDAAASVARERMPGFDPRANRIGENAVVGLSMPAWEIDLWGRLAARSEAARR
                     EVLASAALADGVRTSLVAQVSSLYLDLLDLDAQDEITRRTLDGRRKALRLTRARFDEG
                     VSSILDVRQAESLLAGSEQALADQARRIAQTENALAILLGRNPGPIARSARLDELRTP
                     ARLEAGLPAELLQRRPDIRAAEQNLRAAGASVEAARKAFLPRITLTTLLGFASTDLGQ
                     LFDSDRYAWSLQPTLGLPLFDGGRLQAGVEVAEAQQRILVEQYKATIRQAFREVADAL
                     VAFERLTEQRAATRRLVHANRERLRVSKARYLAGISSYFEVIDAERQLFDSELGLAQA
                     TRGAQQAVVQLYRALGGGAAESSASNT"
     gene            complement(5765..8818)
                     /locus_tag="PKV97_07235"
     CDS             complement(5765..8818)
                     /locus_tag="PKV97_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB05706.1"
                     /translation="MNIAEYTIRKSVVAWVATLLVLAGGWLSYERLGRFEDPEFVIRQ
                     AVVVTAYPGASPAQVAEEITDRIEGAVQQMQEVEEVVSVSRAGESLVKVEARLAFSHT
                     QAELDQVWDKLRGKIAEAARALPPGAAEPVVKGDFGDVFALFYAVTGEGYSPRQLYDY
                     VDLLRRELVLVPGVARVALLGERREAIYVEVSSARAAQLGVPLERIYASLRAQNLIAP
                     AGAVDAGPLRLQIQPADGVVSVAALGELVVAGDAASGLLRLRDVAEIRREYVDPPAVL
                     MRHDGQAAIGLGISNVLGGNVVEMGDAVKRRLAELEGQRPVGMELNVVSFQSDSVRAA
                     VAGFVDNLVAAVAIVVVVLLLFMGLRSGLIIGAVLVLTVAGTLIAMWMDGIAMQRISL
                     GALIIALGMLVDNAIVVTDGILVRMQKGEAVGPAAGAVVRGTVWPLLGGTVVGILAFS
                     AIGLSPTDMGEYAGSLFWVILYSMLLSWLFAITLTPLLCAAFLKVKVEAGAREGGLLR
                     AYRRLLQGVLARRVASGVVLLGLLAAGVLAFGHVPPGFMPDSARPQFVVDLALPQGTD
                     IRTTAAVVAEAEAKVSAAPGVSHVTSFIGQGGLRFMLTYSPEDPNPAYGQLLVDVERF
                     EDIAGLIERLQPELEAAFPQASVKVWKFMLGRGGGKKIEAAFRGPDPAVLRRLAEEAK
                     AIMAADPGAVAIQDDWRQAVPVVRPHFSVEAAQRAGVLPSDIAAALERTYVGQNIGVY
                     RDKERLIPILARAPVDERGGVEDLGNAQVFSATAGKYVPVSQFVERVDTVWEDALIRR
                     ENRFPTIKAQCDPPAGQLSAPLFERLRPQIEAITLPPGYVLEWHGEHKASVEANAGLA
                     AAAPFGFAAMVVAVIVMFNGLRQTAVIWLTVPLAVVGVAIGLLLFQAPFEFMAILGFL
                     SLTGMLIKNAIVLVDQIDVEIREGKPRLLALLDASASRARPVFLGAVTTVLGVAPLLV
                     DPFFRSMAVTIMFGLIFATGLTLVVVPLLYASFFRIRGDEAEAGR"
BASE COUNT         1438 a         3401 c         2933 g         1211 t
ORIGIN      
        1 acagcttcca ttaccgcgca cccgacaccc acatgcgcgg caccccgcgc ccgctcttcc
       61 tcgagatcac cttcatcgac cgcgacggcc acgagcgcat caaggcgctg agcgagaccg
      121 gccgccaggt cctcgacccc ggcctgcgcg acatcacccg gcgcgagaac accttcgtgc
      181 gcgccgagac ctactggccg gcactgaagg ccctgcgccc cggcgagatc cacgtctcgc
      241 gcgtgatcgg cgcccaggtg cgcacgcact ggatcggcga ctacaccccc actcgcgcgc
      301 aagccctcgg caaacccttc gcccccgagg actccggcta cgccggactg gagaacccgg
      361 tcggcaagcg cttccgggga ttgatccggt gggcggcccc ggtgctgcgc caggacagga
      421 tcatcggcta cgtcacgctg gcgctcgacc acgcccacct gatggccttc accgacaccc
      481 tgcggcccac gcccgaacgc ttcgccccga tcgccgaccc cgcctcgggc aactacgcct
      541 tcatctggga cgacgaaggc cacaacatct cgcacgcgcg cgactacttc atccacggct
      601 acgaccctgc caccggcgag cgcagcgtgc cctggctcga ccgcgagctc tacgaagcct
      661 ggcgcagcag cggacagtcc tggccgacct tctccgccgc cctgacgcct tatcaggacc
      721 agcgcctcac ccgggagccc caccccgaat ccaccgcctc gggcctggtc gcgctcgact
      781 gccgctacct cgacttctcg ccgcaatgcc agggctggca cgacatgacc gagcacggcg
      841 gctcgggctc cttcaccatc ctgttctcgg ggctgcgcaa gctcaccacg gttgccacca
      901 tcccctacta caccggccag tacggcagga cgccgcgcgg cttcggctac gtcaccatcg
      961 gcgccaatgt cgacgacttc caccgcgcgg ccaccgaatc gggccagcgc atcggcaaaa
     1021 tgatcggcga ggccgacgcg ctcacccagc acgagcgcga caccctggtc ggcgacatcg
     1081 cagaccagct gcgcgggacc accacccgcc tggccgcctc cacgctcgcg atggtgctcg
     1141 cggtgatcct gatcgcgatc tggatggccg gactgctctc gcgccggatc acccacgtgg
     1201 tcgatggcat ccaccgcttc gagcagggcg acctcgacca gcgcctggcc gccgcgggcg
     1261 gcgacgagct cgccgaactg gcccgcgcct tcaaccgcat ggccgacacc gtgcaacgct
     1321 cgatccgcag cctggaggat gcccgccaca ccgccgagca ggccaaccgc atgaagagcg
     1381 agttcctcgc caacatgtcg cacgagctgc gcaccccgct caacggcgtg atcggcttcg
     1441 ccgagctgct cagcatggag ctcgaggacc ccgagcagcg cgagtgcgcg accaccatcc
     1501 acgacagcgc ccgccacctg ctcgcggtgg tcaacgacgt gctcgacctc gccaaggtcg
     1561 aggccaaccg gctcaccctc gtcgcgcaga cgatcgacct cgtccccctg gtcgcctcgg
     1621 tggccgcgct gcacaaggtg aacgccgaag ccaagggcgt ggacctgcgg ctggatctgc
     1681 ccgcccatcc gtgcatcgtg gacgcggacc cggtgcgcct gcgccagatc atcgagaacc
     1741 tgctgtcgaa cgcggtgaag ttcacccgcg aaggccatat cctcgtcggc ctcgccgccg
     1801 ccgcggacga gatctcgatc cgcatcgccg acaccggctg cggcatcgct cgcgacgagc
     1861 aggagcgcgt cttcgcaccc ttctaccagg ccgcatcctt cctcaaccgc gggcacggcg
     1921 gcaccggcct cggactcacc ctgtcgcgtc ggctcgccca ccggatgggc ggcacgatcg
     1981 atctcgactc cgtccccggc aagggatcca ccttcaccct gcgcctcccg cgccacttca
     2041 ccgcccccgc gggcggtcct ccatcatgaa caagctcgcc ttcatcatcg acgacagcgc
     2101 ggtcaacgtc gcggtcgccc gcctgctgct gcgccggctg ggttgggtcg tggaggattt
     2161 cgacagcgcc atcccgatgc tcgcgcgcct cgccgaggtg cagcccgcct tcatgctgct
     2221 cgacatctcc atgcccgacc tcggcggcga ggaagcctgc aggaggatcc gcgccaatgc
     2281 cgcctggaac ggcgtccgcg tcatcgccta caccgcccac gcgatggaga ccgagcgcca
     2341 gcgctacctc gcgggcggct tcgacgccgt catcaccaag ccgatcacga tcggcgggct
     2401 gcacgccgcg gtgggccacg caccgaacga ctgagctgcg agaccgacat gcccccccac
     2461 acgcccgaat ccacctccca cttcgacctc gccgccctgc gcagcgacat gtccgagagc
     2521 cgcgccgccg tgcgcgtcgt gctggagacc ttcgccccct ggctgaccga cacccgccag
     2581 cagctgcgcg cggccatcga gagcgctgaa gccgagccat tgggtcgcgt ggcccaccgc
     2641 ctgcgcggcg ccctcgcgca gctgcgcgcc gcgccggcgg tggccctggt gaagaagctc
     2701 gaggcgcact gcaaggcaaa tcccgaagcc tgcctctcac gggaccatgc cgtgctcgcc
     2761 gaactcgacg ccgaactcga cgccctcgcc gcggaagtcg cagcacagct cgaagcgctg
     2821 cggcggtgag gagtaccgat tccgggcggg cagcgcccag cgccccgcgc acaaaaagcg
     2881 ctttctaatc cgcgcccctg cggctataat gccgcgcttc accggacagg tggatgagtg
     2941 gtttaagtcg cacgcctgga aagcgtgttt gggataatat cccaacgcgg gttcgaatcc
     3001 cgccctgtcc gccagaacac ctgcaatcaa gcgcctttcg gggcgctttt ttgttgcgcc
     3061 acacgattca accctcgtcc cgaagaccct cgagcgggca tcgcctgttc gcggcggtgc
     3121 aggttcgccc gcatacggca ccgtcgcctt gcgcgcaccg ggttgccgaa ctttcccccc
     3181 tcccgctcag cgcgccgggc tgctgagcga ggcggcgatg aacccgtcga tcgacgtcat
     3241 cgcaagggcg aagaccggat ggacgaggga cggtcccggc gaaactccgg cgcgagctga
     3301 cgccccatgt gatcggcccc cgatcgcaag ttccgccatc catgaaccgc tcaggcgagc
     3361 cctgccccct ggaggaccgg cttccagcgt tccttggcga tcttcctgaa gcccttgccc
     3421 ttcgggtgca actcgttcgc ccaatcctct tccgcgagcg taccccggct gtcgacgatc
     3481 cgcagatgcg ccgggtcgcc catcgcgatg cgcccgagca tggcgtggaa cgcatcgatg
     3541 agatagcgca cgcagtcacg ctggagcgct tccggcacgc ccgcgtcgac cagcgccggc
     3601 ttgagccagc cggccttgcc cgcgacgccg cgaccatcgg gaatcgcata gtcatacgag
     3661 tgcatcagga tcaggcagtc gagcgaggta tgggtgtaga tcaggccgat caggcgccgg
     3721 taatgatcgt ccatgcgctc caggaactgg ccgagcccgc cgtccccgct gcggaagcag
     3781 tcgccagccg actgcgcgcc gctgcagtcc aaggcgagca ggggccgcat gtcgttgaac
     3841 cccgcgaagt cgttgccgcc gccgctgatg aacaccgccg acaggctgct gccatatcga
     3901 cgcagcgcac cggtgatccg gtccttgtac ttgccatcga cgtagtcgaa ggcctccgcc
     3961 ccgttcattc cctgcgcgag gatggtgtgc tccttcttct cgacgatcgg tccgaggtag
     4021 ctgatcaggc tgcctccggg aaagggatac cagaaccagg aatctccaat cgccagaatg
     4081 ctcggcatca ccagcgcccg ctgcatcgcg gcttcatccc agtatgcggc catgtcgcgc
     4141 tcctctcgag aagatccatc cgtcctcgcc gggaagcagc ttcgcatatc ccgaccacca
     4201 tcgacgccat ccgctttcaa tcaaggtgac aggtgggcag atgtcaagac acgcggacgg
     4261 acgtgtccga gggaacaccg cagccttcga agctccgctg gatcgtcgag ggcgaagcgt
     4321 tcgaccggcg ccagctaggt gttcgacgca ctcgactccg ccgctccacc ccccagcgcc
     4381 cgatagagct gcaccaccgc ctgctgcgca ccacgcgtgg cctgcgcgag gccgagctcg
     4441 ctgtcgaaga gctggcgctc ggcgtcgatg acctcgaagt agctggagat gccggcgagg
     4501 tagcgcgcct tgctcacgcg caggcgctcg cgattggcgt gcacgaggcg gcgcgtggcc
     4561 gcgcgctgct cggtgaggcg ctcgaaggcg acgagcgcgt ccgcgacctc gcggaaggct
     4621 tgacggatcg tcgccttgta ctgctcgacc aggatgcgct gctgcgcctc ggccacctcc
     4681 acgccggcct gcaggcggcc gccgtcgaag agcggcaggc cgagcgtcgg ctgcagcgac
     4741 caggcgtagc ggtcgctgtc gaagagctgg cccaggtcgg tgctggcgaa gccgagcagg
     4801 gtggtgagcg tgatgcgcgg caggaaggcc ttgcgggccg cctccacgct ggcgccggcg
     4861 gcgcgcaggt tctgctcggc ggcgcgaata tccgggcggc gctgcagcag ctccgcaggc
     4921 aggccggctt cgagccgggc gggcgtacgc agctcgtcga gccgggcgct gcgcgcgatc
     4981 gggccggggt tgcggccgag caggatggcg agcgcgttct ccgtctgcgc gatgcggcgc
     5041 gcctggtcgg cgagcgcctg ctcggatcca gccagcaggg attcggcctg gcgcacgtcg
     5101 aggatggacg acaccccctc gtcgaagcgc gcgcgcgtca gccgcagcgc cttgcgccga
     5161 ccgtcgagcg tgcggcgggt gatctcgtcc tgggcgtcga gatcgagcag gtccaggtag
     5221 agcgaggaga cctgcgcgac caggctggtg cgcacaccgt cggcgagcgc ggcgctggcg
     5281 agcacctcgc ggcgcgcggc ctcgctgcgg gcggcgaggc ggccccacag gtcgatctcc
     5341 caggccggca tcgacagccc gaccacggcg ttctccccga tgcggttcgc gcgtggatcg
     5401 aagccgggca tgcgctcgcg tgccacggat gcggcggcat cgacgcccgg aaacagcgcc
     5461 gcacgctcga cgccgtactg ggcgcgggcg agctcgatgc gctcgatcgc gatgcgcagg
     5521 tcggcgttgg cggcgagcgc ctcctcgatc agcgcgtgca gttcgggcgc gggcaggatc
     5581 tccctccacg gctgcacgcc caggctcgcg gcggcctccg cctgcgccac ctcggcctgc
     5641 cgcggccagc ttgcgggcac aggcacgtcc ggccgcacga aggcggctgg ctggcatgcc
     5701 gccagcagca gtgcgagcgc gggcagcagg gcggaccgcg ggcacccggg cagcgacgcg
     5761 cgtctcatcg ccccgcctcc gcctcgtcgc cgcggatgcg gaagaagctt gcgtacagca
     5821 gcggcaccac caccagggtg agtccggtgg cgaagatgag gccgaacatg atggtcaccg
     5881 ccatgctgcg gaagaaggga tcgaccagca gcggcgccac ccccagcacc gtcgtgaccg
     5941 cgccgaggaa gaccgggcgt gcccggctgg ccgacgcgtc gagcagcgcg agcaggcgcg
     6001 gcttgccctc ccggatctcg acgtcgatct ggtccaccag cacgatcgcg ttcttgatca
     6061 gcatgccggt gaggctcagg aagccgagga tggccatgaa ctcgaagggc gcctggaaca
     6121 gcagcagccc gatcgccacc cccaccaccg cgagcggcac ggtgagccag atcaccgcgg
     6181 tctggcgcag gccattgaac atcacgatca ccgccaccac catcgccgcg aagccgaagg
     6241 gcgccgccgc ggccaggccg gcgttggcct cgaccgaggc cttgtgctcg ccgtgccact
     6301 ccaggacata gcccggcggc agcgtgatgg cctcgatctg cgggcgcagg cgctcgaaca
     6361 gcggcgcgga cagctgccct gcgggcgggt cgcactgcgc cttaatggtc gggaagcggt
     6421 tctcgcgccg gatcagcgcg tcctcccaca ccgtgtcgac gcgctcgacg aactggctca
     6481 ccggcacgta cttgccggcg gtcgcactga agacctgggc gttgccgaga tcctcgacgc
     6541 cgccgcgctc gtccaccggc gcgcgcgcca ggatggggat cagacgctcc ttgtcgcgat
     6601 acacgccgat gttctggccg acgtaggtgc gctcgagcgc ggcggcgatg tccgagggca
     6661 gcacgccggc gcgctgcgcg gcctcgaccg aaaagtgcgg gcgcaccact ggcaccgcct
     6721 ggcgccagtc gtcctggatc gccaccgccc cggggtcggc ggccatgatc gccttggcct
     6781 cttccgccag ccggcgcagc accgccgggt cggggccgcg gaaggcggcc tcgatcttct
     6841 tgccgccgcc gcggccgagc atgaacttcc acaccttgac gctggcctgc gggaaggcgg
     6901 cctcgagctc gggctgcagg cgctcgatga ggccggcgat gtcctcgaag cgctcgacat
     6961 cgaccaagag ctggccatag gccgggttgg ggtcttccgg gctgtaggtg agcatgaagc
     7021 gcagcccgcc ctggccgatg aagctcgtca catggctcac gcccggcgcc gcgctcacct
     7081 tcgcctcggc ctcggcgacc acggcggcag tggttcggat gtcggtaccc tgcggcagcg
     7141 ccaggtcgac gacgaactgc ggccgcgccg aatcgggcat gaatcccggt ggcacgtgac
     7201 cgaaggcgag cacgccggcg gcgagcaacc ccagcagcac cacgccgctc gccacgcgcc
     7261 gggccagcac gccctgcagc aggcggcgat aggcgcgcag caggccgccc tcccgcgcac
     7321 ccgcctcgac cttcaccttc aggaaggcgg cgcacagcag cggcgtcagc gtgatcgcga
     7381 acagccacga cagcagcatc gagtacagga tcacccagaa cagcgagccg gcgtactccc
     7441 ccatgtcggt gggcgacagg ccgatcgcgc tgaaggcgag gatgccgacc acggtgccgc
     7501 cgagcagcgg ccacaccgtc ccacgcacca cggcacccgc agccgggccc accgcctcgc
     7561 ccttctgcat ccgcaccagg atgccgtcgg tgaccacgat cgcgttgtcc accagcatgc
     7621 cgagcgcgat gatcagcgcg ccgagcgaga tgcgctgcat ggcgatgccg tccatccaca
     7681 tcgcgatcag cgtgccggcc acagtcagca ccagcaccgc gccgatgatc agcccgctgc
     7741 gcaggcccat gaagagcagc agcaccacga ccacgatcgc cactgccgcc accaggttgt
     7801 ccacgaagcc cgccaccgcg gcgcgcaccg aatcggactg gaaggagacc acgttcagct
     7861 ccatgcccac cggacgctgg ccttcgagct cggcgaggcg gcgcttgacc gcgtcgccca
     7921 tctccaccac gttgccgccg agcacgttgg agatgcccag tccgatcgcc gcctggccgt
     7981 catggcgcat cagcaccgcg ggcgggtcga cgtactcgcg gcggatctcg gcgacatcgc
     8041 gcagacgcag caggccgctc gcggcgtcgc ccgccaccac cagctcgccg agcgccgcca
     8101 ccgagaccac gccgtccgcc ggctggatct gcaggcgcag cgggccggca tcgaccgcac
     8161 cggcgggcgc gatcaggttc tgcgcgcgca gcgaggcgta gatgcgctcg agcggcacgc
     8221 cgagctgcgc ggcgcgcgcg ctcgagacct cgacgtagat cgcctccctg cgctcgccca
     8281 gcagcgccac ccgcgccacg cccggcacca gcaccagctc gcggcgcagc aggtcgacgt
     8341 agtcatagag ctggcgcggg ctgtagccct cgccggtgac cgcgtagaag agcgcgaaga
     8401 cgtcgccgaa gtcgcccttc accaccggct cggccgcacc cggcggcagc gcgcgcgcgg
     8461 cctcggcgat cttgccgcgc agcttgtccc acacctggtc gagctcggcc tgggtatgcg
     8521 agaaggcgag cctggcctcc accttcacca gcgactcgcc ggcgcgcgac accgagacga
     8581 cctcctccac ctcctgcatc tgctgcaccg cgccctcgat gcggtcggtg atctcctcgg
     8641 ccacctgcgc cggcgaggca ccggggtagg cggtgacgac caccgcctgg cggatgacga
     8701 actccggatc ctcgaagcgg ccgaggcgct cgtaggacag ccagccaccc gccagcacca
     8761 gcagcgtggc cacccaggcg acgacggact tgcggatcgt gtattcagcg atgttcatgc
     8821 gcggacccgg ggctcagcgg gactcgagcg ggcggaccgc catgccttcg cgcagcgcgc
     8881 cgacgcccgc agcgacgacg cgctcgccgg gcgcgagccc ggactccacg acgatgtcgg
     8941 cgccgacgat ggcgccggtg cgcaccgggc ggcgcgcgac ccg
//
LOCUS       DAOQNN010000098         8970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3838_length_8970_cov_10.529445, whole genome shotgun sequence.
ACCESSION   DAOQNN010000098 DAOQNN010000000
VERSION     DAOQNN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3838_length_8970_cov_10.529445"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="PKV97_07240"
     CDS             <1..171
                     /locus_tag="PKV97_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB05707.1"
                     /translation="GEALLRQAMQRLDLSARAYHRVLRVARTLADLAGASRPGAAQVA
                     EAIQYRRSLDSR"
     gene            238..1446
                     /locus_tag="PKV97_07245"
     CDS             238..1446
                     /locus_tag="PKV97_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNB05708.1"
                     /translation="MIRPLTHARLASLLAALSSIGPFAIDAYLPAFPAIAAALGASRL
                     EVQQTLTIYMVALASMVLWHGALADRFGRRRVLVALTVVFALASLVCALAPSIEWLWF
                     GRALQGLSGGAGIVVGRAVVRDLHEGPQAQRLMSRVMLIFALAPALAPMLGAGLLALA
                     GWRAIFFFLALFGLALAWTTWRFLPETLAEEDRQPLHPRPLLRAYLAIFRHPAFMLLA
                     LGVALNFNGFFLYVLSAPVFILEHLSLGSGGFVWLFGPAVGGMMIGSTLSERVAGRWS
                     PVRTVGFGFVLMFAAVVANAAVSGLLVPAVPVSVLPIGLFSCGMAVAMPSMSLLGLEI
                     FPERRGMASSCQSFLQIGFNAASSALVAPLLWSSPLGLASGMGMFTALGLCAWMAWWR
                     LGAHVREGAA"
     gene            1634..2473
                     /locus_tag="PKV97_07250"
     CDS             1634..2473
                     /locus_tag="PKV97_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB05709.1"
                     /translation="MLEVDGLVKAFGGFHAVDGCTLRVAKGEILGLIGPNGAGKTTLF
                     NLVAGALQPTAGTIRFLGEDITALPTDARFHKGLVRTFQIPHEFHNLTARENLMMVPP
                     RQPGENLFANWFAAGKVRAAEEAVRQKADDTLAFLELTHVADERAGNLSGGQKKLLEL
                     GRTMMTDAKLVLLDEPAAGVNRTLLRKLEEKILILNRERGYTFILIEHDMEMIEKLCA
                     PVVCMAEGKVLIQGDFHTVRSDSRVLEAYLGETPTAVEREAAEHDPKVEELREQELHR
                     EDL"
     gene            2473..3177
                     /locus_tag="PKV97_07255"
     CDS             2473..3177
                     /locus_tag="PKV97_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB05710.1"
                     /translation="MALLEMKDVRGGYGDADILHGVSMTVGEREIVVIVGPNGAGKST
                     AMKSVFGLLTIRGGNIVFDGEDITGWAPDRIVQRGICYVPQVDNVFREMTVHENFEMG
                     AFLRRGDLAAAYDRIYGLFPDLKAKRRNLAGDLSGGQRQMVAMGRALMLDPKLLLLDE
                     PTAGLSPKYMEQIFQITRDVRDAGVSILLVEQHAKQALAFCDRGYVLATGANRHEGSG
                     QALLADREVAEMFLGG"
     gene            3193..4062
                     /locus_tag="PKV97_07260"
     CDS             3193..4062
                     /locus_tag="PKV97_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB05711.1"
                     /translation="MSFMDFLNFHIVPGVVLGSIYAVGAIGITLVFGILRFSHFAHGD
                     MATLGAFVALALVTAGLTPWAALPIAMAAVAVVAIVVDKLFYDYLKARPKIMTVMASL
                     GVALMLRAVVQVVFGVDPRTYASGIVRPDEYFGILLRDRELYTLAAVVAIVIGLTLFL
                     RHSRWGKAMRAMSDNPDLARLSGVDNRKVTMLTWAIVGALCAASGFMLGINNELHSMM
                     GWQVLLPMFAAAILGGVGRVEGAVLGGLIVGIAEELSIFILPAQYKAATAFALLLLIL
                     LVRPRGLLNGKVL"
     gene            4062..5006
                     /locus_tag="PKV97_07265"
     CDS             4062..5006
                     /locus_tag="PKV97_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB05712.1"
                     /translation="MDLLGLANYAVFMAILIGIYALLALGLNIQWGFTGLFNAGIAGF
                     FAVGAYTSAILTSLPATGRLGGYELPLVVGWLAAMAAAALIAWPIGKICLRFRSDYLA
                     IATIGIAEIIRLVIRTEGWLTGGVRGVTGVPRPFGDLDYMPSQIAYLAIVATLVLIAY
                     LLVERQVKAPWGRMMRAIRDNELAAAAMGKQVEARRLQAFVFGSALMGLAGALFVHFN
                     RSITPEAIDPMIATFLIWIMLILGGSGNNRGAILGAAVIWIIWSVSELLTDRLPTEVA
                     VQAKYARVFIIGLTLQLVLRFRPEGILPERQAGSPPHR"
     gene            5100..>6241
                     /locus_tag="PKV97_07270"
     CDS             5100..>6241
                     /locus_tag="PKV97_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB05713.1"
                     /translation="MKKRTVAAVVGAMLGAAVVAAPAHAQDVKLGLMAAISGPIAALA
                     PPMAAASKLAVAHVNEQGGILKGGKLEVVLGDSACNPQNATDVATKAVNIDRVIAVVG
                     PACSGAVLASANSVTIPAGVLMITPSGTSPEITKLKDKDLVYRTLPSDDYQGRALART
                     LKARGISKVAVAYLNNDYGKGLAESFKSEFEANGGTIAGYSGHEEGKASYRSELATLA
                     RGGADTLVIFDYGDGTGLSILRQSLENNFFKTFVGADGMKSEGLVKAIGAANLGGFFV
                     SAPVGEASASLDNFNKAFKAAGENIDAVFATTSYDAAFLAALAIEKAGGDKTKLAESL
                     RAVASAPGEPILAGEWAKAKKLIAEGKDIDYKGAGGDHEFDAAGDVP"
     assembly_gap    6242..6341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6342..6737
                     /gene="bamE"
                     /locus_tag="PKV97_07275"
     CDS             <6342..6737
                     /gene="bamE"
                     /locus_tag="PKV97_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNB05714.1"
                     /translation="STSAEVRERFGPPHMEWRNEDGSLTWEFSRQPEGIECFMITIGP
                     DQILRRIDQVLNEETFAKVQHGMNGDQVRRLLGKPASSQFFQLKQETVWEWRISRGPD
                     GTNDPVFFTVSFNNEGRVTGTGRHTQYKR"
     gene            6817..7374
                     /locus_tag="PKV97_07280"
     CDS             6817..7374
                     /locus_tag="PKV97_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05715.1"
                     /translation="MLAVLLAGCASFEPPRPYTTEAEALSARGEPTRRWNNDDGTVTL
                     EYSTQPNGRSCLMVQVDRGGIVLRQWDALAPENLARVERGMDKEQVSRLLGTHRSEQR
                     FRNSGEEVWDWNIRNDGPGIAVLFNVHFIDGKVVRTSQTYVFPRDGAVLGGWSGYYGH
                     PWGWGIGWGYPGPFFPRPFGHPFFW"
     regulatory      7421..7495
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            7571..>8970
                     /locus_tag="PKV97_07285"
     CDS             7571..>8970
                     /locus_tag="PKV97_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNB05716.1"
                     /translation="MQADRSIELRQLLAERILILDGAMGTMIQQEKLGEADYRGERFL
                     DHPKDLKGNNDLLVLTRPDVVAGIHRAYLDAGADLIETNTFNATRVSQAEYGLEALAY
                     ELNVEGARLVRRLCDEYTAKDPARPRFCAGVLGPTSRTLSISPDVNDPGYRNISFDAL
                     ADDYYASAKGLLEGGADILLIETIFDTLNAKAAVFAIEKLFEDLGRRWPVMISGTITD
                     ASGRTLSGQTAEAFWNSLGHAQPISFGLNCALGADQLRQYVEELSTACDTHVSAHPNA
                     GLPNPLSPTGYDETPEHLAGQIREWAQSGLVNIVGGCCGTTPAHIAAIAEAVRGLAPR
                     AVPVLEKKLRLSGLEPFNVGGDSLFVNVGERTNVTGSKAFARMILEGRFDDALAVARQ
                     QVENGAQVIDINMDEAMLDSVAAMERFCKLIATEPDISRVPIMLDSSKWSVIEAGLKC
                     IQGKGVVNSISMKEGEA"
BASE COUNT         1310 a         3108 c         2972 g         1480 t
ORIGIN      
        1 ggcgaagccc tgctgcgcca ggcgatgcag cggctcgacc tgtcggcgcg ggcgtatcat
       61 cgtgtcctgc gcgtcgcgcg cacgctcgcc gatctcgccg gcgcgtcccg tccgggggca
      121 gcccaggtgg ccgaggcgat ccagtaccgg cgcagcctcg actcccgctg aacgaccccg
      181 cgccccgtgc gccgtcggct gcggacgacg acaccaggag tccgcccgaa ggcgtacatg
      241 atccgaccgc tcacccacgc ccgcctcgct tcgctgctgg cagccctgtc ctcgatcggc
      301 ccgttcgcga tcgatgccta tctgcccgcc tttccggcga tcgcggcggc gttgggcgcg
      361 agccggctgg aggtgcagca gacgctgacg atctacatgg tggcgctcgc gtccatggtg
      421 ctatggcacg gtgcgctcgc cgaccgtttc gggcgtcgcc gcgtgctggt cgcgttgacg
      481 gtggtgttcg cgctcgcctc gctggtgtgc gcgctggcgc cgagcatcga gtggctgtgg
      541 ttcgggcgcg cgctgcaggg gctgagcggc ggcgcgggca tcgtggtcgg gcgtgcggtg
      601 gtgcgcgacc tgcacgaggg gccgcaggcg cagcgcctga tgtcgcgggt gatgctgatc
      661 ttcgcgctcg ctccggcgct ggcgccgatg ctgggcgcgg gcctgctcgc gctcgcaggc
      721 tggcgggcga tcttcttctt cctggccctt ttcgggctcg cgctcgcgtg gacgacgtgg
      781 cgcttcctgc ccgagacgct cgccgaggag gaccgccagc cgctgcatcc gcggccgctg
      841 ctgcgcgcct acctggcgat cttccgccac ccggccttca tgctgctggc gctcggggtg
      901 gcgctgaact tcaacggttt cttcctctac gtgctctcgg cgccggtgtt catcctcgag
      961 catctgagcc tgggcagtgg cggtttcgtg tggctgttcg gtccggcggt gggcgggatg
     1021 atgatcggct cgacgctctc cgagcgcgtg gccgggcgct ggtcgccggt gcgcacggtg
     1081 ggcttcggct tcgtcttgat gttcgcggcg gtggtggcca acgccgcggt gtcgggcctc
     1141 ctcgtccccg cggtgccggt ctcggtgctg ccgatcggct tgttcagctg cggcatggcg
     1201 gtggcgatgc cgagcatgag cctgctcggg ctggagatct ttcccgagcg ccgcggaatg
     1261 gcgtcgagct gccagagctt cctgcagatc ggcttcaacg cggcaagctc ggcgctggtg
     1321 gcgccgctgt tgtggtcgag tccgctcggg ctggcgtcgg ggatgggcat gttcaccgcg
     1381 ctcggcctgt gtgcgtggat ggcatggtgg cggctgggcg cgcacgtgcg cgagggtgcg
     1441 gcgtagcaag gtcgcgccac gcccgtagtg gttggcccct atttttgtcg cggatgcatt
     1501 cggctaaggt cacgcctcgt tctagtatcc gtgtgttatc gaccgccctg ctcggggcgt
     1561 cacgttcatg tgccggtggg ccggctcacg ccgcctctgt cgcgctcttc tcgccgcaag
     1621 gaatcgcaac gctatgctcg aagtggatgg cctggtcaaa gccttcggcg gcttccatgc
     1681 cgtggatggc tgcaccctgc gggtggccaa gggcgagatc ctcggcctca tcggccccaa
     1741 cggcgcgggc aagaccacgc tgttcaacct ggtcgccggc gccctgcagc cgaccgcggg
     1801 caccatccgc ttcctcggcg aggacatcac cgcgctgcct accgacgcgc gcttccacaa
     1861 aggcctggta cgcaccttcc agatccccca cgagttccac aacctcaccg cgcgcgagaa
     1921 tctgatgatg gtgccgccgc ggcaacccgg cgagaacctc ttcgccaact ggttcgccgc
     1981 cggcaaggtg cgcgcggccg aagaggcggt gcgccagaag gccgacgaca cgctcgcctt
     2041 cctggaactg actcacgtcg ccgacgagcg cgcgggcaac ctgtccggcg ggcagaagaa
     2101 actgctcgag ctcggccgca cgatgatgac cgacgccaaa ctggtgttgc tcgacgagcc
     2161 cgccgccggt gtcaaccgca ccctgctgcg caagctcgag gagaagatcc tcatcctcaa
     2221 ccgcgagcgc ggctacacct tcatcctcat cgagcacgac atggagatga tcgagaagct
     2281 gtgcgctccg gtggtatgca tggccgaggg caaggtgctg atccagggcg atttccacac
     2341 cgtgcgctcc gattcccgcg tgctcgaggc ctacctcggc gagacgccga ccgcggtcga
     2401 gcgcgaggct gccgagcacg accccaaggt cgaggaactg cgcgagcagg aactgcaccg
     2461 ggaggacctg tgatggcgct cctcgaaatg aaggacgtgc gcggcggcta cggcgacgcc
     2521 gacatcctgc acggggtgtc gatgaccgtg ggcgagcgcg agatcgtcgt catcgtcggc
     2581 cccaacggcg ccggcaagtc caccgcgatg aaatccgtgt tcggcctgct cacgatccgc
     2641 ggcggcaata tcgtcttcga cggcgaggac atcaccggct gggcgcccga ccgcatcgtg
     2701 caacgcggca tctgctacgt gccgcaggtg gacaacgtgt tccgcgagat gacggtgcac
     2761 gagaacttcg agatgggcgc cttcctgcgc cgcggcgacc tcgccgccgc ctacgatcgc
     2821 atctacgggc tcttccccga cctcaaggcc aagcgccgca accttgccgg cgacctttcc
     2881 ggcggccagc gccagatggt ggcgatgggg cgcgcgctga tgctcgatcc caagctgctg
     2941 ctgctcgacg aacccaccgc cggcctctcc cccaagtaca tggaacagat cttccagatc
     3001 acccgcgacg tgcgcgacgc cggtgtctcc atcctgctcg tcgagcagca cgccaagcag
     3061 gcgctcgcct tctgcgaccg cggctacgtg ctcgccaccg gcgccaaccg ccacgagggc
     3121 agcggacagg ccctgctcgc ggatcgcgag gtggccgaaa tgttcctcgg cggctaaggg
     3181 agtccggcgc tcatgagctt catggacttc ctcaacttcc acatcgtgcc cggcgtcgtg
     3241 ctcggctcga tctacgcggt gggcgcgatc gggatcacgc tggtgttcgg catcctgcgc
     3301 ttctcccact tcgcccacgg cgacatggcc acgctgggcg ccttcgtcgc gctcgccctg
     3361 gtcaccgccg ggctcacccc ttgggcggcg ctgccgatcg cgatggcggc ggtcgcggtg
     3421 gtggcgatcg tcgtcgacaa gctgttctac gactacctca aggcacggcc caagatcatg
     3481 accgtgatgg cctcgctcgg cgtcgcgctg atgctgcgcg cggtggtgca ggtggtgttc
     3541 ggcgtcgatc cgcgcaccta cgccagcggc atcgtgcgcc cggacgaata cttcggcatc
     3601 ctgctgcgcg accgcgagct gtacacgctg gccgcggtgg tggcgatcgt gatcgggctg
     3661 accctgttcc tgcgccattc gcgctggggc aaggcgatgc gggcgatgtc ggacaacccc
     3721 gacctcgcgc ggctttcggg cgtggataac cgcaaggtca ccatgctcac ctgggcgatc
     3781 gtcggcgcgc tgtgcgcggc ctcgggcttc atgctcggca tcaacaacga gctgcactcg
     3841 atgatgggct ggcaggtgct gctgccgatg ttcgccgccg ccatcctcgg tggtgtgggt
     3901 cgggtcgagg gcgcggtgct cggcgggctg atcgtgggca tcgccgagga gctgtcgatc
     3961 ttcatcctgc cggcgcagta caaggcggcc accgccttcg cgctgctgct gctgatcctg
     4021 ctcgtccgcc cgcgcgggct gctcaacggc aaggtgctct gatggatctg ctcggactcg
     4081 ccaactacgc ggtcttcatg gccatcctga tcggcatcta cgcgctgctc gcgctcgggc
     4141 tgaacatcca gtggggcttc accggccttt tcaacgccgg catcgcgggc ttcttcgcgg
     4201 tcggcgccta cacctccgcg atcctcacca gcctgcccgc caccggccgg ctgggcggtt
     4261 acgagctgcc gctcgtggtc ggctggctgg cggcgatggc ggcggcggcg ctgatcgcgt
     4321 ggccgatcgg caagatctgc ctgcgcttcc gctccgacta cctcgccatc gccaccatcg
     4381 gcatcgccga gatcatccgc cttgtgatcc gcaccgaagg ctggctcacc ggcggcgtgc
     4441 gcggcgtcac cggcgtgccc cgtcccttcg gcgacctcga ctacatgccc tcgcagatcg
     4501 cctacctggc gatcgtcgcc accctggtgc tgatcgccta cctgctcgtc gagcgccagg
     4561 tgaaggcgcc gtgggggcgc atgatgcggg cgatccgcga caacgagctc gccgccgcgg
     4621 cgatgggcaa gcaggtcgag gcgcgccggc tgcaggcctt cgtgttcggc tcggcgctga
     4681 tggggctggc gggagcgctg ttcgtgcact tcaaccgctc gatcaccccc gaggcgatcg
     4741 accccatgat cgccactttc ctcatctgga tcatgctgat cctgggcggc tcgggcaaca
     4801 accgcggtgc catcctcggc gcggcggtca tctggatcat ctggtcggtg tccgagctgc
     4861 tcaccgaccg cctgcccacc gaggtcgcgg tgcaggccaa gtatgcgcgc gtgttcatca
     4921 tcggcctgac cttgcagctg gtgctgcgct tccgccccga gggcatcctg cccgagcgcc
     4981 aggcgggttc gccgccgcat cgttgagctg cgcgcgaccg tgaccagaac ttcgtggcgg
     5041 gcccgcggcg ggcccgcatc gtccatcagg tgcttcggca cattcaggag gtgacaaagg
     5101 tgaagaaaag aacagtcgca gcagtggtcg gcgccatgct cggcgccgcg gtcgtcgccg
     5161 caccggccca cgcccaggac gtcaagctcg gcctgatggc ggccatctcg ggcccgatcg
     5221 cggcgctcgc gccgccgatg gcggcggcct ccaagctggc ggtcgcccac gtcaacgaac
     5281 agggcggcat cctcaagggc ggcaagctcg aggtcgtgct cggcgacagc gcctgcaacc
     5341 cgcagaacgc caccgacgtc gccaccaagg cggtgaacat cgaccgcgtg atcgcggtcg
     5401 tcggtccggc ctgctcgggc gcggtgctcg cctcggcgaa ctcggtgacc atccccgccg
     5461 gcgttctgat gatcacccct tccggcacct cgcccgagat caccaagctc aaggacaagg
     5521 acctcgtcta ccgcacgctg ccgtccgacg actaccaggg ccgtgcgctg gcgcgcaccc
     5581 tcaaggcgcg cggcatctcc aaggtggcgg tggcctacct gaacaacgat tacggcaagg
     5641 gcctggccga atccttcaag tccgagttcg aggccaacgg cggcaccatc gccggctatt
     5701 cgggccacga ggagggcaag gcctcctacc gctccgagct ggccacgctc gcccgcggtg
     5761 gcgccgacac cctggtcatc ttcgattacg gcgatggcac cggcctgagc atcctgcgcc
     5821 agtcgctcga gaacaacttc ttcaagactt tcgtcggcgc cgacggcatg aagtccgagg
     5881 gtctggtcaa ggcgatcggc gcggccaacc tcggtggctt cttcgtgtcc gccccggtgg
     5941 gcgaggcctc ggcctcgctc gacaacttca acaaggcctt caaggccgcg ggcgagaaca
     6001 tcgacgccgt gttcgccacc acctcctacg acgccgcctt cctcgccgcg ctggcgatcg
     6061 agaaggcagg gggcgacaag accaagctgg ccgagtcgct gcgcgcggtg gccagtgcgc
     6121 cgggcgagcc gatcctggcg ggcgagtggg ccaaggccaa gaagctgatc gccgaaggca
     6181 aggacatcga ctacaagggc gccggcggcg accacgagtt cgatgccgcc ggcgacgtgc
     6241 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntccacctcc gccgaggtgc
     6361 gcgagcgctt cggcccgcca cacatggaat ggcgcaacga ggacggctcg ctcacctggg
     6421 agttcagccg tcagccggag gggatcgagt gcttcatgat caccatcggc cccgaccaga
     6481 tcctgcgccg catcgaccag gtgctgaacg aggagacctt cgccaaggtc caacacggca
     6541 tgaatggcga ccaggtgcgt cgcctgcttg gcaagccggc gtccagccag ttcttccagc
     6601 tcaagcagga gacggtgtgg gagtggcgga tctcgcgcgg tcccgacggc acgaacgatc
     6661 cggtcttctt cacggtgtcg ttcaacaacg aagggcgagt gaccggaacc ggaagacata
     6721 cccagtacaa gcgttagcag ggaggcctcg atgtatttcc ccagcacccc gtccgtccgc
     6781 gcccaggctt caccgctgtg ctggctgtgg cctgtattgc tggccgtcct gctcgcaggt
     6841 tgcgcgagct tcgagccgcc gcgtccctac accaccgagg ccgaggccct gtccgcgcgc
     6901 ggtgagccca cgcggcgctg gaacaacgac gacggcacgg tcacgctgga gtattccacc
     6961 cagcccaacg gtcgcagctg cctgatggtg caggtcgacc ggggcgggat cgtgctgcgc
     7021 cagtgggatg cgctcgcccc cgagaatctc gcccgcgtcg agcgcggcat ggacaaggag
     7081 caggtctcgc gcctgctcgg cacccaccgc tccgagcagc gcttccgcaa ctcgggcgag
     7141 gaggtgtggg actggaacat ccgcaacgac ggtcctggga tcgccgtcct gttcaacgtg
     7201 cacttcatcg acggcaaggt ggtgcggacc agccagacct acgtgttccc gcgtgacggc
     7261 gccgtcctcg ggggctggag cggctactac ggccatccct ggggctgggg catcggctgg
     7321 ggttatcccg gccccttctt cccgcggccg ttcgggcacc ccttcttctg gtaggccgcc
     7381 gcccttggca gcgggcgcat gctgccgata gaatcctggg cctgccgagg agcgctgcga
     7441 ccccctgatc gatccggggc caggctcggc aaccgatcaa cggcgctcac aaaccttcag
     7501 ccggtttgtg gcgccgttcg catttgtgcg tggcgttgcc cggcgaccgt cgaacgaccg
     7561 aggacatccc atgcaagccg accgcagcat cgaactccgc cagcttctcg ccgagcgcat
     7621 cctgatcctg gacggcgcca tgggcacgat gatccagcag gagaagctgg gcgaggcgga
     7681 ctaccgcggc gagcgcttcc tcgaccaccc caaggacctc aagggcaaca acgatctgct
     7741 ggtgctcacg cgtccggacg tggtcgccgg catccaccgc gcctatctgg acgctggcgc
     7801 cgacctcatc gagaccaaca ccttcaacgc cacccgcgtg tcgcaggcgg agtacggcct
     7861 ggaggcgctg gcctacgagc tcaacgtcga gggcgcccgc ctggtgcgcc ggctgtgcga
     7921 cgaatacacc gcaaaggacc ccgccaggcc gcgcttctgc gccggcgtgc tcgggccgac
     7981 ctcgcgcacg ctgtcgatct cgcccgatgt gaacgatccc ggctaccgca acatcagctt
     8041 cgatgcgctt gccgacgact actacgcctc ggcgaagggc ctgctcgaag gcggtgccga
     8101 catcctgctg atcgagacca tcttcgatac gctgaatgcc aaggcggcgg tgttcgcgat
     8161 cgagaagctg ttcgaggacc tcggccggcg ctggccggtg atgatctccg gcaccatcac
     8221 cgacgcctcg ggccgcacgc tgtcgggcca gaccgcggag gccttctgga actcgctcgg
     8281 ccacgcgcag ccgatcagct tcggcctcaa ctgcgcgctc ggcgccgacc agctgcgcca
     8341 gtacgtcgag gaactctcca ccgcctgcga cacccacgtg tccgcccacc ccaacgccgg
     8401 cctgcccaac ccgctgtcgc ccaccggcta cgacgagacg cccgagcacc tcgccggcca
     8461 gattcgcgag tgggcgcaga gcggcctggt gaacatcgtc ggcggctgct gcggcaccac
     8521 gccggcgcac atcgcggcga tcgccgaagc ggtccggggc ctggcgccgc gcgccgtgcc
     8581 ggtgctggag aaaaagctgc gcctgtccgg gctggagccc ttcaacgtcg gcggcgacag
     8641 tcttttcgtc aacgtcggcg agcgcaccaa cgtcaccggt tccaaggcct tcgcgcgcat
     8701 gatcctcgag ggccgcttcg acgacgcgct cgcggtggcg cgccagcagg tcgaaaacgg
     8761 tgcccaggtc atcgacatca acatggacga ggcgatgctg gattcggtcg ccgccatgga
     8821 gcgcttctgc aagttgatcg ccaccgagcc cgacatctcg cgtgtgccga tcatgctcga
     8881 ctcgtcgaag tggtcggtga tcgaggccgg cctcaagtgc atccagggca agggcgtggt
     8941 caactcgatc tcgatgaagg agggcgaggc
//
LOCUS       DAOQNN010000099         8928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3862_length_8928_cov_11.342049, whole genome shotgun sequence.
ACCESSION   DAOQNN010000099 DAOQNN010000000
VERSION     DAOQNN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3862_length_8928_cov_11.342049"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /gene="tdh"
                     /locus_tag="PKV97_07290"
     CDS             complement(<1..894)
                     /gene="tdh"
                     /locus_tag="PKV97_07290"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNB05717.1"
                     /translation="MKALAKLTPSVGLTLTSVEKPEVGPNDVLIKIKKTAICGTDIHI
                     WKWDEWAQKTIPVPMHVGHEYVGVIAEMGQEVRGFKIGDRVSGEGHITCGFCRNCRAG
                     RRHLCRNTVGVGVNRAGAFAEYLAIPAVNAFKIPDDISDDLAAIFDPFGNATHTALSF
                     NLVGEDVLITGAGPIGIMAVAIARHVGARHVVITDVNDHRLELARKMGATRAVNVSRE
                     NLADVKTELHMVEGFDVGLEMSGVPSAFTSMLEHMNHGGKIALLGIPPSNTAIDWNQV
                     IFKGLEIKGIYGREMFETWYKM"
     gene            complement(928..2184)
                     /locus_tag="PKV97_07295"
     CDS             complement(928..2184)
                     /locus_tag="PKV97_07295"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNB05718.1"
                     /translation="MNKNPATAVEQSGKPQSPARERFLAHLHGVVDQIRADGFYKAER
                     VIDSPQGAQVHLGTGADVLNFCANNYLGLANDPRLVKAAQDGLAEDGFGMASVRFICG
                     TQSVHKQLEASISKFLGTEDTILYGSCFDANGGLFETLLTEEDAVISDELNHASIIDG
                     VRLCKARRYRYRNNDMADLEAQLQAADAAGVRFKLIATDGVFSMDGVIADLKSVCDLA
                     DKYGALVMVDDSHAVGFVGTHGRGTAEYCGVEGRVDIITGTLGKALGGSSGGYTSGHK
                     EIIELLRQRSRPYLFSNTLAPSIAAASIKVMELLASDEGLELCRRVKENGEHFRREMT
                     KLGFTLAGKDHPIVPVMLGDAQLAGRMAEELLKEGIYVIGFSFPVVPKGKARIRTQMS
                     AAHTTAQIDRAVAAFAKVGKALGAIA"
     gene            complement(2338..3576)
                     /gene="sstT"
                     /locus_tag="PKV97_07300"
     CDS             complement(2338..3576)
                     /gene="sstT"
                     /locus_tag="PKV97_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HNB05719.1"
                     /translation="MTTANPTALQRVMRMSLISQIAIGLVLGIALALIAPGATKSVAL
                     LGDLFIAALKAVAPILVFVLVAAAIANHRHGQPTHMRPILVLYLLGTFAAAVVAVIAS
                     FAFPTTLVLDTAAATGNPPGGILGVLKNLLLSVVSNPVRALMEANFIGILAWAIGLGV
                     ALRHAAESTRKMVNDVADAVSSIVQVVIRFAPLGIFGLVAATMGESGFDALLGYGKLL
                     MVIVGCMVFVALVVNPLFVFWKLRTNPYPLVFTCLRESGVTAFFTRSSAANIPINLEL
                     CKRMGLHEDTYSISIPLGATINMAGAAITISVMALAAAHTLGIATDFPTALLLCLVSA
                     LAACGASGVAGGSLLLIPMATSLLGVQADVAMQVVAIGFVISVVQDSCETALNSSTDV
                     LFTAAACAAESGEPLQLDRT"
     gene            complement(3711..5090)
                     /locus_tag="PKV97_07305"
     CDS             complement(3711..5090)
                     /locus_tag="PKV97_07305"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HNB05720.1"
                     /translation="MSISVLDLFKIGVGPSSSHTVGPMRAAHDFVAELQRRGLLDQVR
                     RIEAKLYGSLSATGVGHGTDRAVVAGLMGARPDEVDPDFLVGAVDAVRIDASLPLGGT
                     TAVPFEWTRDLRLLPVSLPYHPNAMRLAAHGEAGVLYENTYYSVGGGFVVDEAQAQSG
                     APAEAAVAVPYPFDTGAELLAQCRRHGLRISELMLANESAWRSEAETRAALLRIWDVM
                     RACVTRGVGHEGVLPGGLNVRRRAPGLYRKLSEQASGRNMISDSMSALDWVNLYALAV
                     NEENAGGGRVVTAPTNGAAGIIPAVMHYYMKFMPGSDERDVENFLLAAAAVGVLCKKN
                     ASISGAEVGCQGEVGSACAMAAAGLAEVLGGTPEQVENAAEIGLEHNLGLTCDPVGGL
                     VQVPCIERNAMASIKAINAAQLALRGDGAHTVSLDQVIRTMRDTGRDMLDKYKETSRG
                     GLAVSFPEC"
     gene            5233..6195
                     /locus_tag="PKV97_07310"
     CDS             5233..6195
                     /locus_tag="PKV97_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB05721.1"
                     /translation="MATSAPSQIHTRLRVFLAVARHLSFTRAGEELSITTGAVSQQIK
                     LLEDWLAQRLFRRLPRRLELTDAGKRLLAAVDPAYAAVELAIGRLRGGALSGVVRVRT
                     LPSFLATWLVPRLPRLMARFPQIELQVEAEDSTYSLRDGEFDLAIDLNDGSYPGFQST
                     VLLEEEIFPVCSPRLLEGRPPLLGAEDLRHYPLLHDMTAWRGSPPYAEWERYLKAIGA
                     PQVEVRRGYMFNRNHITMQCAIAGMGVAIARRTLITDELDSGRLVAPFAQSVRTGKRY
                     CVIHSAGALADPRVATVHDWLAAEARAAELAAGPCADTAPAPGD"
     gene            complement(6224..7522)
                     /locus_tag="PKV97_07315"
     CDS             complement(6224..7522)
                     /locus_tag="PKV97_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNB05722.1"
                     /translation="MEVMIAAVLIGLMLALLAGGVWIGLALMAIGILGMLGFTPRAPG
                     DGMAVAIWSHGSSWTLTALPLFLWMGEILFRTKLSEDMFKGLSPWLERLPGRLLHTNI
                     IGCTLFAAVSGSSAATCATIGKIALPELQRRGYPESMALGTLAGAGTLGLLIPPSIIM
                     IVYGVAADVSISKLFIGGVLPGILLASLFMGWVVVWSLMNPGKIPAADLKTDFMGKLR
                     ASKNLIPVILLIGGVLGSIYSGIATATEAAAIGVIGSLIIAASQRTLNRKSFFGALMG
                     ATRLYCMIALILAGSAFLTLAMGYIGLPRHLAEWIGGLGLTPGMLLVALAIFYILLGC
                     FLDGISIVVLTMAVLMPTIQAAGIDPLWFGIFVVVVVEMAQVTPPVGFNLFVLQGLTG
                     RDMTVIARYALPYFLLMVLAVVLLYTFPGLVTWLPGHMIG"
     gene            complement(7535..8053)
                     /locus_tag="PKV97_07320"
     CDS             complement(7535..8053)
                     /locus_tag="PKV97_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNB05723.1"
                     /translation="MRAFLDSLYRLSGALAAVGMVATLVLVATGVFTRPLGIYLPGTD
                     DYAGYAMAACGFLALAYTFKHGEHIRVSLVLERVGPRLRAALEWFSLAAGTAVAAMLA
                     FYAVRMVLQSHEFEEISQGVDATPLWIPQLSMAFGSVVLLIALIDDLVMTTLGRAPAR
                     LATHSGEAARME"
     gene            complement(8112..>8928)
                     /locus_tag="PKV97_07325"
     CDS             complement(8112..>8928)
                     /locus_tag="PKV97_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNB05724.1"
                     /translation="GKLKITVHANGSLFKANEIKRAVQSGQAQIGELIISGLANEDAL
                     YALDTVPFLATGYADSKKLWQASKAAVEARLAKNGLKVLYSVAWPPQGLYSMTELKTI
                     GDLKGMKIRSYSPTVARMIELMGGQPVTVQAADLTQALSTGVVSANLTSSATGYDTKS
                     WENLSHYYDIQAWLPKNIVFVSKKAFDALDQPSRDALLKAAAAAEERGWKVSEEKTAW
                     YIDQLKANKMKVQPAPEALQAELLKVGEKMTAEWVEKAGPDGQAILDAYRKLK"
BASE COUNT         1435 a         3000 c         3104 g         1389 t
ORIGIN      
        1 catcttgtac caggtctcga acatctcgcg gccgtagatg cccttgatct ccaggccctt
       61 gaagatgacc tggttccagt cgatcgcggt gttggacggc ggaatgccca gcagcgcgat
      121 cttgccgccg tggttcatgt gctcgagcat cgaggtgaac gccgacggca cgcccgacat
      181 ctcgaggccg acgtcgaagc cttcgaccat gtgcaactcg gtcttcacgt cggcgaggtt
      241 ctcgcggctc acgttcacgg cgcgcgtcgc gcccatcttg cgggcgagct ccaggcggtg
      301 atcgttgacg tccgtgatga cgacatggcg cgcgccgacg tgacgcgcga tcgccaccgc
      361 catgatgccg atcgggccgg cgccggtgat gagcacgtcc tcgccgacca ggttgaagga
      421 cagcgcggta tgggtggcgt tgccgaaggg gtcgaagatc gccgcgaggt cgtcggagat
      481 gtcgtcgggg atcttgaacg cattgaccgc cgggatcgcc aggtactcgg cgaacgcgcc
      541 cgcgcggttc acgccgacgc ccacggtgtt gcggcagagg tggcgacggc cggcgcggca
      601 gttgcggcag aaaccgcagg tgatgtgccc ctcgcccgac acgcggtcgc cgatcttgaa
      661 gccgcgcacc tcctggccca tctcggcgat cacgccgacg tactcgtggc cgacgtgcat
      721 ggggacggga atggtcttct gcgcccactc gtcccacttc cagatatgga tgtccgtgcc
      781 gcagatcgcg gtcttcttga tcttgatgag gacgtcgttg ggtccgactt ccggcttttc
      841 cacgctggtc agcgtgagcc ccaccgacgg ggtcaatttg gccaaggcct tcatgggatt
      901 cctctcaatc gatatcagtg gtcgtgctca cgcaatcgcg ccgagtgcct tgcccacctt
      961 ggcaaaggcc gccaccgcgc ggtcgatctg cgccgtagtg tgcgcggcgc tcatctgcgt
     1021 gcggatgcgg gccttgccct tgggcacgac cgggaaggag aagccgatga cgtagatgcc
     1081 ttccttcagc agctcttcgg ccatgcgacc ggccagctgc gcatcgccga gcatgaccgg
     1141 gacgatgggg tgatccttgc ccgccagcgt gaagccgagc ttggtcatct cgcggcggaa
     1201 atgttcgccg ttttccttga cgcgacggca cagttcaaga ccttcgtcac tcgcgagcag
     1261 ctccatcacc ttgatcgagg ccgcggcgat ggagggcgcg agcgtgttgg agaacagata
     1321 gggccgcgag cgctggcgca gcagttcgat gatctccttg tgccccgagg tatagcctcc
     1381 cgaggagccc cccagcgcct tgccgagcgt gccggtgatg atgtccacgc ggccttccac
     1441 gccgcagtat tccgccgttc cacggccgtg cgtgccgacg aagccgacgg catgggagtc
     1501 gtcgaccatc accagcgcgc catatttgtc ggccaggtcg cagaccgact tcaggtcggc
     1561 gatcacgccg tccatggaga acacgccgtc ggtggcgatc agcttgaagc gcacgccggc
     1621 ggcgtccgcg gcctgcagct gcgcctcgag gtcggccatg tcgttgttgc ggtagcggta
     1681 gcgcctggcc ttgcacaggc gcacgccgtc gatgatggag gcgtggttga gctcgtcgga
     1741 gatgaccgcg tcctcttcgg tgagcagggt ctcgaacagg ccgccgttgg cgtcgaagca
     1801 gctgccgtac aggatggtgt cctcggtgcc gagaaacttc gagatgctcg cctcgagctg
     1861 cttgtgcacc gactgcgtgc cgcagatgaa gcgcaccgac gccatgccga agccgtcctc
     1921 ggccaggccg tcctgggcgg ccttgaccag gcgcgggtcg ttggcgagcc cgaggtagtt
     1981 gttggcgcag aagttgagga cgtccgcacc ggtgccgagg tggacctggg caccctgagg
     2041 gctgtcgatg acgcgctcgg ccttgtagaa gccgtcggcg cggatctgat cgacgacacc
     2101 gtgcagatgg gcgaggaagc gctcgcgcgc cggggattgg ggcttgccgc tctgttcgac
     2161 ggcagttgcg ggattcttgt tcatgacgac tccgttgcag tgcccgggga ttggaagccc
     2221 cccgggcagg gcttgattca gggaaggcgc ggggccggag ccccgccacg cgcggaggcc
     2281 gcgacggccg ccgcacggac acctgcgccg tggcgcaggc gcacggtcat gccgggatca
     2341 ggtgcgatcc agctggagcg gctcgccgct ctccgccgcg caggcggcgg cggtgaagag
     2401 gacgtcggtc gaggagttca gcgcggtctc gcacgagtcc tgcaccacgc tgatgacgaa
     2461 gccgatcgcc accacctgca tcgccacgtc ggcctggacg cccagcaggc tggtggccat
     2521 cgggatcagc agcagcgagc cccccgccac gcccgaggcg ccgcatgcgg cgagcgcgga
     2581 caccaggcac agcagcagcg cggtcgggaa gtcggtcgcg atgccgaggg tgtgcgccgc
     2641 ggcgagcgcc atcaccgaga tcgtgatcgc cgcgccagcc atgttgatcg tggcgccgag
     2701 cgggatcgag atcgaatagg tgtcctcgtg caggcccatg cgcttgcaca gctcgaggtt
     2761 gatggggatg ttggcggccg agctgcgggt gaagaaggcg gtgacgccgc tctcgcgcag
     2821 gcaggtgaac accagcggat aggggttggt gcgcagcttc cagaacacga acagcgggtt
     2881 gaccaccagg gcgacgaaga ccatgcagcc cacgatcacc atcagcagct tgccgtagcc
     2941 gagcagcgcg tcgaagccgg actcgcccat ggtggcggcg accaggccga agatgccgag
     3001 cggggcgaag cggatcacca cctgcacgat cgaggacacc gcatccgcga catcattgac
     3061 catcttgcgg gtgctctccg cggcatggcg cagcgcgacg ccgaggccga tcgcccacgc
     3121 caggatgccg atgaaattgg cttccatcag cgcgcgcacc ggattggaga cgacgctcag
     3181 cagcaggttc ttgagcaccc cgaggatgcc gcccggcggg ttgccggtcg cggccgcggt
     3241 gtcgagcacc agcgtggtcg ggaaggcgaa gctggcgatc accgccacca ccgcggcggc
     3301 gaaggtgccg agcaggtaga ggacgaggat cgggcgcatg tgcgtgggct ggccgtgcct
     3361 gtggttggcg atcgccgccg ccaccagcac gaagaccagg atcggcgcga ccgccttcag
     3421 cgcggcgatg aagaggtcgc cgagcagcgc gaccgacttg gtggcgcccg gcgcgatcag
     3481 cgccagtgcg atgccgagga ccagaccgat ggcgatctgg gagatgaggc tcatgcgcat
     3541 cacgcgctgc agagccgtgg ggtttgcggt ggtcattgag tgtctcctcg tggatgacgt
     3601 tgtggttagg gaagccgggt gaggctggac ccgggcacgt gcgggatggg ctgtgccggg
     3661 tcgggggtgg aacgggtgga cattcggctg gagatccggt gtggcggcgc tcagcactcc
     3721 ggaaagctga cggcgaggcc gccgcgggag gtctccttgt acttgtcgag catgtcgcgg
     3781 cccgtgtcgc gcatggtccg gatcacctgg tcgagcgaga ccgtgtgcgc gccgtcgccg
     3841 cgcagggcga gttgggcggc gttgatggcc ttgatcgagg ccatcgcgtt gcgctcgatg
     3901 cagggcacct gcaccaggcc gccgaccggg tcgcaggtca ggccgaggtt gtgctcgagg
     3961 ccgatctcgg cggcgttctc gacctgctcg ggcgtgccgc cgagcacttc ggccaggccc
     4021 gcggcggcca tcgcgcaggc cgagccgacc tcgccctggc agccgacctc ggcgccggag
     4081 atggaggcgt tcttcttgca cagcacgccg accgcggcgg cggcgaggag gaagttctcc
     4141 acgtcgcgct cgtcgctgcc gggcatgaac ttcatgtagt agtgcatcac cgccgggatg
     4201 ataccggccg caccattggt gggcgccgtg acgacgcggc cgccgccggc gttctcttca
     4261 ttgaccgcga gcgcgtagag gttgacccag tcgagcgcgc tcatcgagtc gctgatcatg
     4321 ttgcgcccgc tcgcctgctc gctgagcttg cgatacagcc ccggcgcgcg ccggcgcacg
     4381 ttgagcccgc cgggcagcac gccctcgtgg cccacgccgc gcgtcacgca ggcgcgcatc
     4441 acgtcccaga tgcgcagcag cgcggcgcgc gtctccgcct cgctgcgcca ggccgactcg
     4501 ttggccagca tcagctcgct gatgcgcagg ccgtggcggc ggcactgggc gagcagctcg
     4561 gcgccggtgt cgaagggata gggcacggcg accgcggcct cggccggcgc gcccgactgc
     4621 gcctgcgcct cgtcgaccac gaagccgcca cccaccgagt agtaggtgtt ctcgtacagc
     4681 acgccagcct cgccgtgcgc ggccaggcgc atcgcgttgg gatggtaggg caggctcacc
     4741 ggcagcaggc gcaggtcgcg cgtccactcg aagggcaccg ccgtcgtgcc gcctagcggc
     4801 aggctggcat cgatgcgcac cgcatcgacc gcgcccacca ggaagtcggg atcaacctcg
     4861 tccgggcgcg cgcccatcag gccggccacc accgcgcggt cggtgccgtg gccgacgccg
     4921 gtcgccgaca gcgagccgta gagcttcgcc tcgatgcggc gcacctggtc gagcaggccg
     4981 cggcgctgca gctcggcgac gaaatcgtgc gcggcccgca tcggcccgac cgtgtgcgag
     5041 ctggacggac cgacgccgat cttgaacagg tcgagtacgc tgatggacat gggagactcc
     5101 gaggggaaac ggcttgttga tttaattctg ctaagcacga aaatacttat cagtgcatga
     5161 gcggcattct ccagctggat gggcgggtgc tcaatcgata gttattgatc ttttctttag
     5221 caaaactaaa ccatggctac gagcgcaccc tcccagatcc atacccggct acgcgtcttc
     5281 ctcgccgtcg cgcgtcacct gtccttcacc cgcgccggcg aggagctcag catcaccacc
     5341 ggcgcggtga gccagcagat caagctgctc gaggactggc tcgcccagcg cctgttccgc
     5401 cgcctgccgc gccggctcga gctcaccgac gcgggcaagc gcctgctcgc cgcggtcgat
     5461 ccggcctacg ccgcggtcga gctcgccatc ggccgcctgc gtggcggcgc gctcagcggc
     5521 gtggtacggg tgcgcaccct gccctccttc ctcgccacct ggctggtccc gcgtctgccg
     5581 cgcctgatgg cgcgctttcc gcagatcgag ctgcaggtcg aggccgagga cagcacgtac
     5641 tcgctgcgcg acggcgagtt cgacctcgcg atcgacctca acgacggcag ctaccccggc
     5701 ttccagagca ccgtgctgct ggaagaggag atcttcccgg tgtgctcgcc acgcctgctc
     5761 gaaggccgtc caccgctgct cggcgccgag gacctgcgcc actacccgct gctgcacgac
     5821 atgaccgcct ggcgcgggag cccgccctac gccgagtggg agcgctacct gaaggccatc
     5881 ggcgcacccc aggtggaggt acggcgcggc tacatgttca accgcaacca catcaccatg
     5941 cagtgcgcga tcgccggcat gggggtggcg atcgcgcgcc gcaccctgat caccgacgag
     6001 ctcgacagcg gccgcctggt ggcgcccttc gcgcagtcgg tgcgcaccgg caagcgctac
     6061 tgcgtgatcc attccgcggg ggcgctcgcc gatccgcgcg tggccacggt gcacgactgg
     6121 ctggcggccg aggcgcgtgc ggcggagctc gccgccggcc cttgcgcaga tacggcgccc
     6181 gcgcccggtg actgaacgcc agcggccgcg ggcgcggagc ccgtcacccg atcatgtgcc
     6241 cgggcagcca ggtcaccagg ccggggaagg tgtacagcag caccaccgcc agcaccatca
     6301 gcaggaagta gggcagcgcg tagcgcgcga tcaccgtcat gtcgcgcccg gtgaggccct
     6361 gcagcacgaa gaggttgaag ccgaccggcg gggtgacctg cgccatctcc accaccacca
     6421 ccacgaagat gccgaaccac agcggatcga tgcccgcggc ctggatcgtc ggcatcagca
     6481 ccgccatcgt cagcaccacg atcgaaatgc cgtcgaggaa gcagccgagc aggatgtaga
     6541 agatcgccag cgccaccagc agcatgcccg gggtgaggcc gagcccgccg atccactcgg
     6601 cgagatggcg gggcaggccg atgtagccca tcgcgagggt caggaaggcc gagccggcga
     6661 ggatcagcgc gatcatgcag tacagccggg tggcgcccat cagcgcaccg aagaagctct
     6721 tgcgattgag cgtgcgctgg ctggcggcga tgatcaggct gccgatcacg ccgatcgcgg
     6781 cggcctcggt cgcggtggcg atgccgctgt agatcgagcc gagcacgccg ccgatcagca
     6841 ggatcaccgg gatcaggttc ttcgaggcgc gcagcttgcc catgaagtcg gtcttgaggt
     6901 cggcggcggg gatcttgccc gggttcatca gcgaccacac caccacccaa cccatgaaca
     6961 ggctcgccag caggatgccg ggcagcacac caccgatgaa gagcttggag atcgacacgt
     7021 ccgccgccac gccgtacacg atcatgatga tcgagggcgg gatcagcagg cccagcgtgc
     7081 cggcacccgc cagggtgccc agcgccatcg actcggggta gccgcggcgc tgcagctcgg
     7141 gcagcgcgat cttgccgatg gtggcgcagg tcgccgccga cgagcccgac accgcggcga
     7201 acaaggtgca gccgatgatg ttggtgtgga gcaggcggcc cggcaggcgt tccagccagg
     7261 gcgacaggcc cttgaacatg tcttccgaca gcttggtgcg gaagaggatc tcgcccatcc
     7321 acaggaacag cggcagcgcg gtcagggtcc agctcgaacc gtggctccag atcgccaccg
     7381 ccatgccgtc gcccggcgcg cgcggcgtga agccgagcat gccgaggatg ccgatcgcca
     7441 tcagcgcaag gccgatccat acgccgccgg cgagcagcgc cagcatcagg ccgatcagta
     7501 cggccgcaat catgacttcc atggcgcgct cctcttattc catgcgcgcg gcttcgccgc
     7561 tgtgggtggc gagtcgcgcc ggtgcgcgcc ccagggtggt catcaccagg tcgtcgatca
     7621 gcgcgatcag cagcacgacc gagccgaagg ccatcgacag ttgcggaatc cacagtggcg
     7681 tggcgtccac gccctgcgag atctcctcga attcgtgcga ctgcagcacc atgcgcacgg
     7741 cgtagaaggc cagcatcgcc gccaccgcgg tgccggcggc aagcgagaac cactccagcg
     7801 ccgcgcgcag gcgcgggccg acgcgctcga gcaccaggct cacgcggatg tgctcgccgt
     7861 gcttgaaggt gtaggccagc gcgaggaagc cgcaggccgc catcgcgtag ccggcgtagt
     7921 cgtcggtgcc gggcaggtag atgccgagcg ggcgcgtgaa gacgccggtc gcgaccagca
     7981 ccagcgtggc caccatgccg accgcggcga gcgccccgga caggcggtac aaggaatcga
     8041 ggaacgctct catgggcgat ctcctgaaca agaaggcccc gccacggcgg ggccgatgcg
     8101 gcatccgccg gtcacttcag cttgcggtag gcgtcgagga tggcctggcc gtccggaccg
     8161 gccttctcga cccactcggc ggtcatcttc tcgccgacct tgagcagctc ggcctgcagc
     8221 gcctccggcg cgggctggac cttcatcttg ttggccttga gctggtcgat gtaccaggcg
     8281 gtcttctctt ccgacacctt ccagccgcgc tcctcggccg cggcggcggc cttcagcagg
     8341 gcatcgcgcg acggctggtc gagcgcgtcg aaggccttct tcgacacgaa cacgatgttc
     8401 ttcggcagcc aggcctggat gtcgtagtag tggctcaggt tttcccagct cttggtgtcg
     8461 taaccggtgg ccgaggaggt caggttggcc gacaccacgc cggtcgacag cgcctgggtg
     8521 aggtcggcgg cctgcacggt caccggctgc ccccccatca gctcgatcat gcgcgccacc
     8581 gtcgggctgt aggagcggat cttcatgccc ttgaggtcgc cgatggtctt gagctcggtc
     8641 atggagtaca ggccctgcgg cggccacgcc accgaataca gcaccttcag gccgttcttc
     8701 gccaggcgtg cctcgaccgc cgccttcgag gcctgccaca gcttcttcga atccgcatag
     8761 ccggtggcca ggaagggcac ggtgtcgagc gcgtagagcg cgtcctcgtt ggccagcccg
     8821 gagatgatca gctcgccgat ctgcgcctgc ccggactgca ccgcgcgctt gatctcgttg
     8881 gccttgaaca gcgagccgtt ggcgtgcacg gtgatcttga gcttgccg
//
LOCUS       DAOQNN010000100         8825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3926_length_8825_cov_8.246180, whole genome shotgun sequence.
ACCESSION   DAOQNN010000100 DAOQNN010000000
VERSION     DAOQNN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8825
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3926_length_8825_cov_8.246180"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKV97_07330"
     CDS             complement(<1..285)
                     /locus_tag="PKV97_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB05725.1"
                     /translation="MSIIERALAKAKQEDRAAVEQAEGLVHRPQAGQDAQGAVAERTG
                     DVADHPAVAAAVEAEIEPAALAPAPAAAGARSVRDARAAAPLLYVEDLRCA"
     gene            complement(282..1313)
                     /locus_tag="PKV97_07335"
     CDS             complement(282..1313)
                     /locus_tag="PKV97_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB05726.1"
                     /translation="MNVPHIDRMNAGGDLPLLSVRNLRVEFPRRRGTLVALDDVSFDI
                     ARGEVLGVVGESGAGKSLTGAAIIGLLEPPGRIAGGDILLDGEAIHALRGEAMRRLRG
                     RRIAMIFQDPLTSLNPLYTVGEQLVETMLTHLDLTPAAARERALALLDEVGIPAPAQR
                     IDHYPHQFSGGMRQRVVIALALCAEPELIIADEPTTALDVSVQAQIIALLRRLCRQHR
                     TAVMLITHDMGVIAETADRVAVMYAGRVVEIGPVAEVVRAPAHPYTRGLMGAIPVLGA
                     EVERLVQIDGAMPRLDAIPSGCAFHPRCTEASARCRVERPELLPAGATRAACWLYAPA
                     RENTDGGPQ"
     gene            complement(1310..2269)
                     /locus_tag="PKV97_07340"
     CDS             complement(1310..2269)
                     /locus_tag="PKV97_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB05727.1"
                     /translation="MSTESPTPTTAAAPRGALARLLDSDLLHGFLRSPITVLSALIVL
                     AILIAALLAPVIAPQNPFDPATLNLMDGFSRPMQANAFTGNVYWLGTDAQGRDLFSAI
                     LYGSRVSLLVGFAAVAFAAVLGIALGLIAGYRGGWVDSLIMRIADVQLSFPAILVALL
                     IFGVFKGVVPPALHDKAAIYVLILAIGLSDWVQYARTVRSSTLAERNKEYVQAARVIG
                     VPAPTILLRHILPNVMGPVLVIATIGLALAIILESTLSFLGVGVPPTQPSLGTLIRVG
                     QDYLFSGEWWIVFFPGLTLLLLALSVNLLGDWLRDALDPRLRR"
     gene            complement(2296..3279)
                     /locus_tag="PKV97_07345"
     CDS             complement(2296..3279)
                     /locus_tag="PKV97_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB05728.1"
                     /translation="MPAFILRRLLQSVLVMLTVALVAFGLFRFIGDPVNNMVGQEATM
                     AERAALRESLGLDDPVPVQFLRFVVNAAQGEFGVSYRMARPVADVIAERLPATLELAL
                     ISGFGALALGIALGVYTALRRDGWLSHAIMSLSLVGVSLPTFLIGILLIYVFAVELKL
                     LPAFGRGEVVDLGWWTTGLLTTSGLKSLVMPAITLGLYQLTLIMRLVRAEMLEVLRTD
                     YIKFARARGLTDRTVHFGHALRNTLLPVITITGLQIGSIIAFAIITETVFQWPGVGLL
                     FITAVQFVDIPVMAAYLVLIALIFVVINLIVDLLYFVVDPRLRVQRTGRGQ"
     gene            complement(3447..5042)
                     /locus_tag="PKV97_07350"
     CDS             complement(3447..5042)
                     /locus_tag="PKV97_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB05729.1"
                     /translation="MNKTRLALALPFALATLAGVAAAPAAAQTVRWAAAGDALTMDPH
                     SQNEGPTHVMNHQVYDSLVFRDQAMKLAPRLATAWKITEDPNVWEFKLREGVKYHNGN
                     PFTADDVVFSIQRAKHENSDMKGLLTSVVEVVKVDEHTVRMRTDGPNPLLPNNLTNLF
                     IMDREWSEANKVMLPQNYKAGDETFAVRNANGTGPFRLVKREPDVRTELERNEDYWGK
                     GTYPMEVAKVVFTPVRSAATRVAALLSGEVDFLLDPPVQDLERLSAAKGIVVRSGPEN
                     RTIFLGMNQGAAELRSADVKGRNPFADKRVRAAMNIAINRDAVKRVVMRGQSVPAGIV
                     APPFIDGYDKAMDVVPAPDVARAKALLAEAGYPNGFAVTLSCPNDRYVNDEAICQAVT
                     GMFGQIGVKARLDARPKSIHFAELPKGELDLYMLGWGVPTMDSHYVFHYLYETRTDKG
                     GSWNVTGYSSAKVDELTKAMNREIDLGKRAGMVAEVWKTVQDDVVYLPIHHQMLNWAM
                     KDDIDFPVQSENYPYFKLLKYRK"
     gene            complement(5258..5719)
                     /locus_tag="PKV97_07355"
     CDS             complement(5258..5719)
                     /locus_tag="PKV97_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNB05730.1"
                     /translation="MDFHPLLLFVHVAGVVVWVGGMFFAYVCLRPEAASQLEPPPRLR
                     LWRGVLARFFTWVWVAVAAVLASGLALLLRIGFAAAPPHQHLMLLMGLVMIAIYVFVY
                     SVPYAALSKAVAAEEWKAGGAALGRIRQLVGTNLTLGFLTIAMGTIGRWLG"
     gene            complement(5802..6434)
                     /locus_tag="PKV97_07360"
     CDS             complement(5802..6434)
                     /locus_tag="PKV97_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNB05731.1"
                     /translation="MSTTRIVVIAKAPQPGRVKTRLIPALGAEGAAALAARMLACTLV
                     AAFEAAGAADTADPALGVVELCASPAPGDPAWQGVVVPAGLRWSAQGEGDLGARMAGA
                     ARRVLAGGERVLLIGTDCPALDAACLRTAAEALDEVEAVIVPSFDGGYVLLGLRRFDP
                     SLFEGIAWSTSTVAAATRVRLDALAWSYQVLPALQDIDVPADLVHLPSAG"
     gene            complement(6431..7135)
                     /locus_tag="PKV97_07365"
     CDS             complement(6431..7135)
                     /locus_tag="PKV97_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNB05732.1"
                     /translation="MSAAASDMEGRPVSLSIVVPMVDEAATLPELLTHLAAWRDRGCE
                     IVLVDGGSRDDSVAIARAAGFRVLVAERGRARQMNAGAAACGGDILLFLHADTRLPED
                     ADAKLRAVQAGHAWGRFDVRIDGRPRMLRVVAALMNLRSRSSGIATGDQAIFVRREVF
                     EAVGGFPDQPLMEDIELSRRLLRVSRPACLRARVRTSGRRWEQRGVWRTIVLMWRLRW
                     AYWRGVPAERLAEAYR"
     gene            complement(7132..8145)
                     /gene="arsS"
                     /locus_tag="PKV97_07370"
     CDS             complement(7132..8145)
                     /gene="arsS"
                     /locus_tag="PKV97_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327695.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNB05733.1"
                     /translation="MHATLPLLESHRFPPLRREAITTLQVNLGYRCNQSCVHCHVNAG
                     PGRTEMMDADTLALIPRVLAARAIGTLDLTGGAPELHPGFRALVREARALGVRVIDRC
                     NLTILSEPGQEDLAEFLAEHGVEVVASLPCYAADNVDRQRGKGVFERSIAGLLRLNAL
                     GYGQAGSGRVLNLVYNPQGPVLPPDQARLEADYRRELAAQFGIVFNQLFALANMPIQR
                     FGSMLISKGQFNAYMALLQASHRDENLGGVMCRTLVSVDWQGRLYDCDFNQQLGLPLG
                     AEGALTSAQGHDDSDAAARSGRLHLRDLLAGDLAGAPIRVADHCFGCTAGSGSSCGGA
                     LKA"
     gene            8322..>8825
                     /locus_tag="PKV97_07375"
     CDS             8322..>8825
                     /locus_tag="PKV97_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB05734.1"
                     /translation="MKRLLLVGGGHAQLAVLQALARRRPHGVEAVLLTPSPWQWYSGM
                     LPGWMAGHYRAEDCRIDLRALADAAGVRLCLASAIALDAERRMLLDDTGTTHDFDLLA
                     LDVGSLVDTRALDALPTDALLAVKPLEAFIAAWPQRLAMARAGQLRRLAVVGGGAAGV
                     ELVLAAAE"
BASE COUNT         1365 a         3132 c         3109 g         1219 t
ORIGIN      
        1 ggcgcagcgc aggtcctcga cgtacagcag cggcgccgcg gcacgcgcat cgcgcacact
       61 gcgcgcgcca gcagcagccg gcgccggtgc aagcgctgcg ggctcgatct cggcttcgac
      121 cgcggccgcc acggcgggat ggtccgccac atcgcccgtc cgctccgcga ccgctccctg
      181 cgcgtcctgc ccggcctgcg gccgatgcac caggccctcg gcctgctcga ccgcggcgcg
      241 gtcctcctgc ttcgccttgg cgagcgcgcg ctcgatgatg ctcactgcgg gcctccgtcg
      301 gtgttctcgc gcgccggcgc gtacaaccag cacgccgcgc gcgtggcgcc ggccggcagc
      361 aactccgggc gctcgacgcg gcagcgcgcg ctggcctcgg tgcagcgcgg atggaaggcg
      421 cagccggacg ggatcgcatc caggcgcggc atcgcgccgt cgatctgcac caggcgctcg
      481 acctcggcgc cgagcaccgg gatggcgccc atcaggccgc gggtgtaagg atgcgccgga
      541 gcgcgcacca cctcggccac cggcccgatc tccaccaccc gcccggcgta catcaccgcg
      601 acgcggtcgg cggtctcggc gatcacgccc atgtcgtggg tgatcagcat caccgcggtg
      661 cggtgctggc ggcacaggcg acgcagcagc gcgatgatct gcgcctgcac cgagacgtcg
      721 agcgcggtgg tgggctcgtc ggcgatgatc agctcgggct cggcgcacaa ggcgagcgcg
      781 atcaccacgc gctggcgcat gccgccggag aactggtggg gataatggtc gatgcgctgc
      841 gccggcgccg ggatgccgac ctcgtcgagg agcgcgagcg cacgctcgcg cgcggcggcg
      901 ggcgtcaggt cgaggtgggt cagcatggtc tcgaccagct gctcgcccac cgtataaagc
      961 gggttcaggc tggtgagcgg atcctggaag atcatcgcga tgcggcgtcc gcgcaggcgc
     1021 cgcatggcct cgccgcgcag cgcgtggatg gcctcgccat cgagcaggat gtcgccgccg
     1081 gcgatacgcc cggggggctc gagcaggccg atgatggccg cgccggtgag cgacttgccc
     1141 gcacccgact cgcccaccac tccgagcacc tcgccgcgcg cgatgtcgaa ggagacgtcg
     1201 tcgagcgcca ccagcgtccc gcgccggcgg ggaaactcca cgcgcaggtt gcgtaccgac
     1261 agcagcggca ggtcgccgcc ggcgttcatc cggtcgatgt gcggcacgtt catcggcgca
     1321 gcctcgggtc gagcgcgtcg cgcagccaat cgccgagcag gttcaccgac agcgcgagca
     1381 gcagcagcgt cagcccgggg aagaacacga tccaccactc gcccgagaac aggtagtcct
     1441 ggccgacgcg gatcagggtg ccgaggctgg gctgggtggg cggcacgccc acgccgagga
     1501 aggacagcgt cgactccagg atgatcgcca gcgcgaggcc gatggtggcg atcaccagca
     1561 ccggccccat cacgttgggc aggatgtggc gcagcaggat ggtgggcgcc ggcacgccga
     1621 tcacgcgcgc ggcctgcacg tactccttgt tgcgctcggc cagcgtggag ctgcgcaccg
     1681 tgcgcgcgta ctgcacccag tccgacaggc cgatcgccag gatcagcacg tagatcgccg
     1741 ccttgtcgtg cagcgccggc ggcaccacgc ccttgaacac gccgaagatc agcagcgcca
     1801 ccaggatcgc cgggaagctc agctgcacgt cggcgatgcg catgatcagg ctgtccaccc
     1861 agccgccgcg atagccggcg atcagcccga gcgcgatgcc gagcaccgcg gcgaaggcca
     1921 ccgccgcgaa accgaccagc agcgacaccc gcgagccgta gaggatggcc gagaagaggt
     1981 cgcgcccctg cgcgtcggtg cccagccaat acacgttgcc ggtgaacgcg ttggcctgca
     2041 tggggcggct gaagccgtcc atcaggttca gcgtggcggg atcgaagggg ttctgcggcg
     2101 cgatcaccgg cgcgagcaac gcggcgatca ggatcgccag cacgatcaag gccgagagca
     2161 cggtgatcgg cgagcggagg aagccgtgca ggaggtcgct gtcgagcagg cgtgcgagcg
     2221 caccgcgcgg tgcggcagcg gtggtgggcg tgggggattc ggtgctcatg ctgggttccg
     2281 gggttccggg ggcgctcact gcccgcgccc ggtgcgctgc acgcgcaggc gcggatcgac
     2341 gacgaagtag agcaggtcga cgatcaagtt gatgacgacg aagatcagcg cgatcagcac
     2401 caggtaggcc gccatcaccg ggatgtcgac gaactgcacc gcggtgatga agagcaggcc
     2461 cacgcccggc cactggaata ccgtctcggt gatgatggcg aaggcgatga tcgagccgat
     2521 ctgcagcccg gtgatggtga tcaccggcag cagggtgttg cgcagcgcgt ggccgaaatg
     2581 caccgtgcgg tcggtgagcc cgcgcgcgcg ggcgaacttg atgtagtcgg tgcgcagcac
     2641 ctcgagcatc tcggcgcgca cgaggcgcat gatcagcgtg agctggtaca ggccgagcgt
     2701 gatcgccggc atcaccagcg acttcagccc cgaggtggtc agcagcccgg ttgtccacca
     2761 tcccaggtcc accacctcgc cgcggccgaa ggcgggcagc agcttcaatt cgaccgcgaa
     2821 cacgtagatc agcaaaatgc cgatcaggaa ggtgggcagc gacaccccca ccagcgacag
     2881 gctcatgatc gcgtggctca gccagccgtc gcggcgcagc gcggtataca cgccgagcgc
     2941 gatccccagc gcgagcgcgc cgaagcccga gatcagcgcc agctccagcg tcgccggcag
     3001 gcgctcggcg atcacgtcgg ccaccggccg cgccatgcgg taggacacgc cgaactcgcc
     3061 ctgcgccgca ttcacgacga agcgcaggaa ctgcaccggc accgggtcgt cgaggccgag
     3121 cgactcgcgc agcgccgcgc gctcggccat ggtcgcctcc tgaccgacca tgttgttcac
     3181 cgggtcgccg atgaagcgga acagcccgaa cgcgaccagc gccaccgtca gcatgacgag
     3241 gacggactgc agcaggcggc gcaggatgaa ggcgggcatt cgggggagcg ggagtgagga
     3301 atcggatgcg gggtgcgagg gaggggatgg gagcgtgatc gcgggcgggg gagcggggga
     3361 tcgcgactgg cgtcgcgcct acagggcggt caccgcgtcc ggtgagcttt ctgtaggagc
     3421 gacgcaagtc gcgatcccgc gggtcatcac ttcctgtact tcaacagctt gaagtaggga
     3481 tagttctccg actgcaccgg gaagtcgatg tcgtccttca tcgcccagtt cagcatctgg
     3541 tggtggatcg gcaggtagac gacgtcgtcc tgcaccgtct tccacacttc ggcgaccatg
     3601 ccggcgcgct tgccgaggtc gatctcgcgg ttcatcgcct tggtcagctc gtccaccttc
     3661 gcgctggaat agccggtcac gttccacgag ccgcccttgt cggtcctggt ctcgtagagg
     3721 taatggaaga cgtagtgcga gtccatggtc ggcacacccc agccgagcat gtagaggtcg
     3781 agctcgccct tgggcagctc ggcgaagtgg atgctcttgg gccgtgcgtc cagccgcgcc
     3841 ttgacgccga tctggccgaa catgccggtc accgcctggc agatggcctc gtcgttcaca
     3901 tagcggtcgt tggggcagga cagcgtcacc gcgaagccgt tcgggtagcc ggcctcggcg
     3961 agcagcgcct tggcgcgcgc gacgtcgggc gccggcacga cgtccatcgc cttgtcgtag
     4021 ccgtcgatga agggcggcgc gacgatgccg gcgggcaccg actggccgcg catcaccacg
     4081 cgcttcaccg cgtcgcggtt gatcgcgatg ttcatcgccg cgcgcacgcg cttgtccgcg
     4141 aaggggttcc tgcctttcac gtccgcgctg cgcagctccg ccgcgccctg gttcatgccg
     4201 aggaagatcg tgcggttctc cggcccggag cgcaccacga tccccttggc cgcggacagg
     4261 cgctcgaggt cctgcaccgg cgggtcgagg aggaaatcga cctcgcccga gagcagcgcc
     4321 gcgacccggg tggcggccga gcgcaccggg gtgaacacca ccttggccac ctccatggga
     4381 taggtcccct tgccccagta gtcctcgttg cgttcgagtt cggtgcgcac gtcgggctcg
     4441 cgcttcacca gccggaaggg accggtgccg ttggcattgc gcacggcgaa ggtctcgtcg
     4501 ccggccttgt agttctgcgg cagcatgacc ttgttcgcct cggaccactc gcggtccatg
     4561 atgaagaggt tggtcaggtt gttgggcagc agcgggttgg ggccgtcggt gcgcatgcgc
     4621 acggtgtgct cgtcgacctt gaccacctcg accacgctgg tgagcaggcc cttcatgtcc
     4681 gagttctcgt gcttggcacg ctggatggag aacaccacgt cgtcggccgt gaaggggttg
     4741 ccgttgtggt acttcacgcc ctcgcgcagc ttgaactccc acacgttggg gtcctcggtg
     4801 atcttccacg cggtggccag ccgcggcgcc agcttcatgg cctggtcgcg gaacaccagc
     4861 gagtcgtaga cctggtggtt catgacgtgg gtcgggccct cgttctgaga atgcgggtcc
     4921 atggtcagcg cgtccccggc cgccgcccac cgcacggtct gtgcagccgc gggggcggcc
     4981 gccacgccgg cgagcgtcgc gagggcgaaa ggcagggcga gggcaagtcg ggtcttgttc
     5041 atttttcggt ctccgtcggg tgctgccggg attgaggaac gggtgggccg ggggactgcc
     5101 ggaccggctc ggcaccgagc atgccggtcg attgttgcac cggccgggcc ggccggccaa
     5161 gcacgcagcg tcaggatcag ggttctcccc gatccggccg ttgcgacgag cggtgtgcat
     5221 gggtggccac cgccccgccg cgaacgggcg ctgcggctca gcccagccag cgcccgatcg
     5281 tacccatggc gatggtgagg aagccgagcg tcaggttcgt ccccaccagc tgccggatgc
     5341 gcccgagcgc cgcaccgccc gccttccact cctcggccgc gaccgccttg ctcagggctg
     5401 cgtagggcac ggaatagacg aacacataga tcgcgatcat caccaggccc atcagcaaca
     5461 tgaggtgctg gtgcggcggc gccgccgcga agccgatgcg cagcagcaac gccaacccgc
     5521 tcgccagcac cgcagccacc gcaacccaaa cccaggtgaa gaagcgcgcc agcaccccgc
     5581 gccacagccg caagcgcggc ggtggctcga gctggctggc ggcctcggga cgcaggcaga
     5641 cgtaggcgaa gaacatcccg cccacccaga cgacgacgcc ggcgacgtgc acgaaaagca
     5701 gcagcggatg gaagtccatg gggattctcc ttcgtgggat cgggaacccg attgtgccgc
     5761 aaaagccggc ccgcaccggc ggatcccggc ttgccccggc ttcatccggc ggatggaaga
     5821 tggacgaggt cggccgggac gtcgatgtct tgcagcgcgg gcaacacctg atacgaccac
     5881 gccagcgcat cgaggcgcac ccgggtcgcc gccgcgaccg tcgatgtact ccaggcgatg
     5941 ccctcgaaca ggctcgggtc gaagcggcgc aggccgagaa gcacgtagcc gccgtcgaag
     6001 ctcggcacga tcaccgcctc gacctcgtcg agcgcctcgg ccgcggtgcg caggcaggct
     6061 gcgtcgagcg ccgggcagtc ggtgccgatc agcagcacgc gctcgccgcc ggcaagcacc
     6121 cgccgcgccg cgcccgccat gcgtgcgccg agatcgccct caccctgcgc ggaccagcgc
     6181 aggcctgccg ggaccacgac gccctgccag gccggatcgc ccggggcggg gctggcgcac
     6241 agctcgacca cgccgagcgc tggatcggcg gtatcggcag ccccggcggc ctcgaaagcc
     6301 gccaccagcg tgcacgccag catgcgggca gccagcgccg cggctccctc cgcaccgagc
     6361 gccgggatca ggcgcgtctt cacccgcccc ggctgcggcg ccttggcgat caccacgata
     6421 cgggtcgtgc tcaccggtag gcctcggcga ggcgctcggc cggcacgccg cgccagtagg
     6481 cccagcgcag gcgccacatc agtacgatgg tgcgccacac cccgcgctgc tcccagcggc
     6541 gccccgaggt ccgcacccgc gcccgcaggc aggccgggcg cgacacgcgc agcagccggc
     6601 gggagagctc gatgtcctcc atcagcggct ggtccgggaa accgcccacc gcctcgaaca
     6661 cctcccggcg cacgaagatg gcctggtcgc cggtggcgat cccgctcgag cgcgagcgca
     6721 ggttcatcaa cgctgccacc acgcgcagca tgcgcgggcg cccgtcgatg cgcacgtcga
     6781 aacggcccca ggcatggccg gcctgcacgg cccgcagctt cgcgtccgca tcttccggca
     6841 ggcgcgtgtc cgcatgcagg aacaacagga tgtctccgcc gcacgccgcc gcacccgcgt
     6901 tcatctgcct ggcgcggcca cgctcggcga ccagcacgcg gaagccggct gcgcgcgcga
     6961 tcgccaccga gtcgtcgcgg ctgccgccgt ccaccagcac gatctcgcag ccgcggtcgc
     7021 gccaagcggc gagatgggtg agcagttcgg gcagcgtggc cgcctcgtcc accatcggca
     7081 cgacgatgga gagcgaaacc gggcgcccct ccatgtcgga cgccgccgca ctcacgcctt
     7141 cagcgccccg ccgcagctcg aaccgctgcc cgcggtgcag ccgaagcagt gatcggcgac
     7201 gcggatcggt gcgccggcga gatctcccgc gagcaggtcg cgcagatgca gtcggcccga
     7261 gcgcgccgcc gcgtcgctgt cgtcgtgccc ctgcgcggac gtgagcgccc cttccgcccc
     7321 cagcggcagg ccgagctgct ggttgaagtc gcagtcgtac agccgcccct gccagtccac
     7381 gctgaccaag gtgcggcaca tcaccccgcc gagattctcg tcgcggtggc tggcctgcag
     7441 cagcgccatg taggcgttga actgcccctt cgagatcagc atcgagccga agcgctggat
     7501 cggcatgttg gcgagcgcga acaactggtt gaagacgatg ccgaactgcg ccgcgagctc
     7561 gcgccggtag tcggcctcga gccgggcctg gtcgggcggc agcaccggcc cctgcgggtt
     7621 gtacaccagg ttcagcaccc ggccgctgcc ggcctggccg tagccgagcg cattcaggcg
     7681 cagcaggccg gcgatgctgc gctcgaacac gcccttgccg cgctggcggt cgacgttgtc
     7741 cgcggcgtag cagggcagcg aggccaccac ctcgacgccg tgctcggcga ggaattcggc
     7801 gagatcctcc tgccccggct cggacaggat ggtgaggttg cagcggtcga tcacgcgcac
     7861 cccgagcgcc cgcgcctccc gcaccagcgc acggaagccc ggatgcagct cgggcgcgcc
     7921 gccggtgagg tcgagcgttc cgatcgcgcg cgcggcgagc acgcgcggga tcagcgccag
     7981 cgtgtcggcg tccatcatct cggtccggcc ggggccggcg ttgacgtggc aatgcacgca
     8041 gctctggttg cagcgatagc cgaggttcac ctgcagcgtg gtgatcgcct cgcggcgcag
     8101 cggcggaaag cggtgcgatt cgagcagcgg gagggtggca tgcatgcgga gtgtctccat
     8161 cgggaaagtc gattctttcg gcacctgcgg aatccgcggc agccggcacg gtcttttgtc
     8221 gtacctgcct cggaaaagct tacgctcggg aagttcgaac cgttcccctc gtccgtcccc
     8281 ccctcacccc cccatccgcg cctccttgtc cgcccgaaac catgaagcga ctcctcctcg
     8341 tcggcggcgg ccacgcccag ctcgcggtcc tccaggccct cgcccgacgg cgtccgcacg
     8401 gggtggaggc ggtgctgctg acgccctcgc cctggcaatg gtactcgggg atgctgcccg
     8461 gctggatggc cggccattac cgcgccgagg actgtcgcat cgacctgcgc gcgctcgccg
     8521 acgcagccgg tgtgcggctc tgcctcgcct ccgcgatcgc gctcgacgca gaacgtcgca
     8581 tgctgctgga cgacaccggc accacgcacg acttcgacct gctcgcgctc gacgtcggca
     8641 gcctcgtcga cacgcgcgcg ctcgacgccc tgccgacgga cgccttgctg gcggtgaagc
     8701 cgttggaagc cttcatcgcc gcctggccgc agcggctggc catggcgcgc gccgggcaac
     8761 tgcgccggct cgccgtggtg ggcggcggcg cggcgggggt ggagctcgtg ctcgccgccg
     8821 cggag
//
LOCUS       DAOQNN010000101         8775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_3965_length_8775_cov_7.356307, whole genome shotgun sequence.
ACCESSION   DAOQNN010000101 DAOQNN010000000
VERSION     DAOQNN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3965_length_8775_cov_7.356307"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKV97_07380"
     CDS             <1..365
                     /locus_tag="PKV97_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05735.1"
                     /translation="AGAGAAGLLREAERLLTQAQTSTPELGARYRLMAQMLRERAAVA
                     DPDGLVRLVTDRVATVEASTPDLVVRHDENGQPVTVAEEMARVRREAAEGTDAELGAL
                     DADLLRVAAECALTRGTA"
     gene            complement(349..543)
                     /locus_tag="PKV97_07385"
     CDS             complement(349..543)
                     /locus_tag="PKV97_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05736.1"
                     /translation="MGQFLPLLLFALACSAVLLFAGLGAAGNLRGALRYMLIWVKCVG
                     ALALVGVVLSLIVSLFTPSP"
     gene            542..3079
                     /locus_tag="PKV97_07390"
     CDS             542..3079
                     /locus_tag="PKV97_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05737.1"
                     /translation="MAMHPDCVRTVRAAASGRQISDAKMQALEDALGASMRDLARADR
                     QRWHGLTRDQRMAEATAKAMQDVQAEAALKEYRAGLQVLRTAESETRITEQMRLVGAT
                     RSKGLIHDIENTSDYVHAVRNESIAALADMIDAAQTRDGTGVLRNLGMRILDLDNPQM
                     TGDVVREVFASADGHTGNRAAQAGAQAWLDVIEKMRQRFNAAGGAIGKLGYGYLAQAH
                     DAARVAKAGAEGWAREVLPLLDREQYVRADGTLLGDAELLGVLQAAHETIATGGDNKV
                     EPGQYRGSGARANHGSDHRVLHFKDGDAWIAYMKAFGEGSLYDAMVGHVGRMARDIGL
                     VERYGPNPEQQFRLQNDLAERADGIGTAANRGAGNTPDAYWSILSGASGSPENRTVAF
                     WGQNVRNVQTAAKLGGAMLTSITDLGTVAASLHYNRLPYFDMLANLGRRLKPGSEESD
                     FLQAHGVIGEALTTTLNRWTGDHMTHSLTGRVAGSVMKLSLLNAWTDGLRGAFAATMM
                     QGFAKKLGKAWGELDAWDRHLMQRKGITEADWSIITRAVPTERNGLAYLTHDAVAGVN
                     EALSREAATKWLAFVSDEAQFAVINPDMATRAIVTAGGMPAGTVAGEAMRSFMQFKSF
                     PIAMLTRHWRRVFETPQGLDGAPAGYGAASAGGAAVNRIALLAALNVTLTMLGAVVLQ
                     TKSLVQGKDPYDMTEGKFWMRANTQGGGMGYVGDLIFKDPTEQRSSSVEQTVGSILGP
                     AAGAAAGLVGDLGVVNAWEAAKGKDTHIAAEALRWTNSQLPYGNLWQTRAAWEHWALF
                     NLQEAVNPGYLSRMQRRAQKDWGQGYWWAPGAALPDSAPDFGHAVGR"
     gene            3084..3443
                     /locus_tag="PKV97_07395"
     CDS             3084..3443
                     /locus_tag="PKV97_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05738.1"
                     /translation="MRTDQTKRLAELAEQLVDAFITEADPATWPGAGHESDMTQRERG
                     DRYWSKKNASATGGVLRFALDLIGRTGADDPSAGGDEEDDLDKRIRQAERRASDALAR
                     AMKRASAPGDGRATRTG"
     gene            3418..5070
                     /gene="terL"
                     /locus_tag="PKV97_07400"
     CDS             3418..5070
                     /gene="terL"
                     /locus_tag="PKV97_07400"
                     /inference="COORDINATES: protein motif:HMM:NF033889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HNB05739.1"
                     /translation="MAARRAPVDFATFFALWAEEQGWDVPDVHWRAVHWLQHRGALAV
                     LLCFRGFGKSTLLAVYNAWRYYADPSYRILHQGDQDRTAYKTSRDTRSVLQRHPLTRA
                     QPALALRGEQSFWWVAASADERNPSMQAAGITSNITSSRCDEAQNDDVEVPRNIQNPE
                     AREKMRYRLGEQTHCMVPGARQLFVGTPHTHDSLYDEMTRAGADRLAIPMFAREKRFE
                     SVGAGVLEVGFVPELVFSGIGAEARLLTRGKDYALSGSSLTLSAPAALLDCYAGSAWP
                     ERFGSKEMLARRRKCKTVNEWDSQYQLHSRPLHEMRLDPARLRRYSAEPVVRVANGEL
                     GMWLGDARIVAAAARWDPSSGKVTSDASSLSVMLQDEIGRRYWHRVLEMRGEVAQTSE
                     DGKTIIGGQVTEIVRVVRELHLPRVSVETNGIGGFAPAFLKAAFKQARLVCGVSEVQS
                     TANKNRRILEAFEGPLKSGQLWAHDSVLDGPTWNQMRDWNPVVPSQPDDHLDSGAGAL
                     VETPERITRAVERAGSAGLNAPAVPSENWRPSAGMHEVELEF"
     gene            5110..7170
                     /locus_tag="PKV97_07405"
     CDS             5110..7170
                     /locus_tag="PKV97_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05740.1"
                     /translation="MAVTEQTPISTYTANGVATAFAFAFLAFEAGSLLVQTTDLTGVT
                     VDAVLGVDYTVTGLGNPAGGSVTFSVAPANGYRVTVSRHSGLHRDTDYQDNGDLLSGT
                     VNIDFDRLWLVLQEIYRGGGGLASALRVPIGEVVAALPNAAARANKTLGFDALGAPLL
                     MLGGGGGGGGGGDVAALQAALADAANVANGDAMLAVKQPFAGSVARTQHDKNIDTGLY
                     PEDFRLPADPNDTLSIQRILDLGQFCYLRPGKTYVAYGLKSKAGTGLVCLGGRATIDV
                     PLAAGNTSGIWIDTGDFILDGVNFIGHETGPWNVTTPTGMANRFGVIVGKLFGTGSGV
                     DGITVRNCDISGFNNSGLQGGEVQVGYNFGKHCTVDNVYCFDNYVNFWYGPRFEYVTT
                     TNCSGYRGWAGIIMQAGNNKFSACQFEKNNENCQLPAGENDGHGTFVGCSFNHSWAGA
                     GPGGYGLHAASIANGHVFIGCQWWFAPILLDTCTGIRISHSEIVSSPVTIVGGGLNMI
                     DDNYVRDSLSRTFSGNTFTSFRRNRTKSTDNTVDEAFGDVYVRHKALTAGFPFALTTG
                     GSAKKIPFSAYWNKYNGLDAAFLTPANGDVYIPRPGRYRVALNIVLNPGASGVVNLLI
                     RRSLSGGGSEDVLNVAETLAAQRSFCLADTLLCENGDTLSAMYYVASATNVVSATFSV
                     SSVD"
     gene            7182..7337
                     /locus_tag="PKV97_07410"
     CDS             7182..7337
                     /locus_tag="PKV97_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05741.1"
                     /translation="MTIRFIPADKCVHAVVGALSGALALAAAAALTIGGAVQALFEVA
                     DNLRADI"
     gene            7394..7594
                     /locus_tag="PKV97_07415"
     CDS             7394..7594
                     /locus_tag="PKV97_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05742.1"
                     /translation="MPGDTDDVPLDGDRDLDARERQVLRKMIRDQERMRWLWRVSRVG
                     VALLAGLATAILWTMDHVKLIK"
     gene            7605..7892
                     /locus_tag="PKV97_07420"
     CDS             7605..7892
                     /locus_tag="PKV97_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05743.1"
                     /translation="MQLPMIPQDKANHAIYGGAIALVAAAIAHVAGAREWQAQIALGA
                     ACAAGVAKEVADYIVARRQRANGDAVTHGVDGWDAAVTALGGMAVAIGVWL"
     gene            7889..8647
                     /locus_tag="PKV97_07425"
     CDS             7889..8647
                     /locus_tag="PKV97_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HNB05744.1"
                     /translation="MSVIDQARPLLSGLLRQWEGLRLSPYRCPAGVPTIGVGATTYLD
                     GRPVTMADQPITEEQALRMLDVECDRYLQAVMDMCTVAPSGNELAALAGLAYNIGLDA
                     LRTSTALRQHNAGNHEAAGRAIALWDKARVGGTLTVLPGLTARRAAERALYLTPASGE
                     VERMPQAVESESPMAASPINRGGAAVVGLGAIEGLRQAGDSIAVVRAPLDALKGLLTD
                     TIGLPPEWLLPVALAAVGAYVVYWRLQQRRQGFA"
BASE COUNT         1462 a         2990 c         3163 g         1160 t
ORIGIN      
        1 gcgctggcgc aggcgccgca gggctgctgc gcgaggccga gcggctgctc acgcaggccc
       61 agaccagcac gccggagctc ggcgcgcgct accgcctgat ggcgcagatg ctgcgcgagc
      121 gcgccgccgt ggccgacccc gacgggctgg tgcggctcgt caccgatcgc gtggccactg
      181 tcgaggccag cacgcccgac ctggtggtgc gccacgacga gaacgggcag ccggtgacgg
      241 tggccgagga aatggctcgc gtgcgccgcg aggcggccga gggcaccgac gccgaactcg
      301 gcgcgctcga cgccgatctg ctgcgcgtgg ccgccgagtg cgccttgact agggggacgg
      361 cgtgaacaag ctcacgatga gcgacagcac gacgccgacg agcgcgagcg cgccgacgca
      421 cttgacccag atcaacatgt accgcagcgc gccgcgcagg ttgccggccg cgcccagccc
      481 ggcgaacagc agcacggccg agcacgcgag cgcgaagagc agcaaaggca ggaactgacc
      541 catggcgatg caccccgatt gtgtgcggac cgtccgcgcg gccgcaagcg gtcgccagat
      601 cagcgacgcg aagatgcagg cgctcgaaga cgcgctcggc gccagcatgc gcgatctggc
      661 gcgcgccgac cgccagcgct ggcatggcct cacgcgcgac cagcgcatgg ccgaggccac
      721 ggccaaggcg atgcaggacg tgcaggccga agccgcgctc aaggagtacc gcgccgggct
      781 gcaggtgctg cgcaccgccg agagcgagac gcgcatcacc gagcagatgc gcctggtcgg
      841 agcgacgcgc tccaagggcc tgatccacga catcgagaac accagcgact acgtgcacgc
      901 ggtgcgcaac gagtcgatcg cggccctggc cgatatgatc gacgcggccc agacgcgcga
      961 cggcaccggc gtgctgcgca acctgggcat gcgcatcctc gacctcgaca acccgcaaat
     1021 gacgggcgac gtggtgcgcg aggtcttcgc cagcgccgac ggccacaccg gcaaccgcgc
     1081 ggcgcaggcc ggcgcccagg cctggctcga cgtgatcgag aagatgcgcc agcgcttcaa
     1141 cgccgccggc ggcgccatcg gcaagctcgg ctatgggtac ctcgcgcagg cgcacgacgc
     1201 ggcgcgcgtg gccaaggccg gcgccgaggg ctgggcgcgc gaggtgctgc cgctgctcga
     1261 ccgcgagcag tacgtgcgcg ccgacggcac gctcctgggc gacgccgagc tgctcggggt
     1321 gctgcaggcc gcgcacgaga cgatcgccac cggcggcgac aacaaggtcg agcccggcca
     1381 gtaccgtggc agcggcgcca gggccaacca cggcagcgat caccgcgtgc tgcacttcaa
     1441 ggacggcgac gcgtggattg cctacatgaa ggcgttcggc gaagggtcgc tgtacgacgc
     1501 gatggtgggc cacgtcgggc gcatggcgcg cgacatcggc ctcgtcgagc gctacgggcc
     1561 caaccccgaa caacagttcc ggctgcaaaa cgacctggcc gagcgcgccg acggcatcgg
     1621 cacggcggcc aaccgcggcg ccggcaacac cccggacgcc tactggtcga tcctgagcgg
     1681 cgcgagcggc tcgcccgaga accgcacggt ggccttctgg ggccagaacg tgcgcaacgt
     1741 gcagaccgcg gccaagctgg gcggcgcgat gctcacctcc atcaccgacc tgggcaccgt
     1801 cgcggccagc ctgcattaca accggctgcc gtacttcgac atgctcgcca acctcggccg
     1861 gcgcttgaag ccgggcagcg aagagagcga cttcctgcaa gcgcacggcg tcatcggcga
     1921 ggcgctcacc accacgctca accgctggac gggcgaccac atgacgcact cgctcaccgg
     1981 gcgcgtcgcg ggcagcgtca tgaagctgtc gctgctcaac gcgtggaccg atggcctgcg
     2041 cggcgcgttc gccgccacga tgatgcaagg cttcgcgaag aagctgggca aggcctgggg
     2101 cgagctcgac gcgtgggacc ggcacttgat gcagcgcaag ggcatcaccg aggcggactg
     2161 gtcgatcatc acgcgggcgg tgccgaccga acgcaacggg ctcgcgtacc tgacgcacga
     2221 cgcggtggcg ggggtgaacg aggcgctctc gcgcgaagcg gccacgaagt ggctcgcctt
     2281 cgtgagcgac gaggcgcagt tcgccgtcat caacccggac atggccacgc gcgccatcgt
     2341 caccgccggc ggcatgccgg ccggcaccgt ggccggcgag gcgatgcgct ccttcatgca
     2401 gttcaagagc ttcccgatcg cgatgctgac caggcactgg cggcgcgtgt tcgagacgcc
     2461 gcaggggctc gacggcgccc cggctggcta cggcgccgcg agcgcaggag gcgccgcggt
     2521 caaccgcatc gcgctgctgg ccgcgctcaa cgtgacgctg acgatgctcg gcgccgtcgt
     2581 gctgcagacc aagtcgctcg ttcagggcaa ggatccttac gacatgaccg agggcaagtt
     2641 ctggatgcgc gccaacaccc agggcggcgg catgggctac gtcggcgacc tgatcttcaa
     2701 ggaccccacc gagcagcgca gcagcagcgt cgagcagacc gtcggctcga tcctgggccc
     2761 ggccgccggc gcggcggcgg ggctcgtcgg cgacctgggc gtcgtcaacg cttgggaggc
     2821 ggccaagggc aaggacacgc acatcgcggc cgaggcgctg cgctggacga actcgcaact
     2881 gccctacggc aacttgtggc agacccgcgc cgcatgggag cactgggcgc tgttcaacct
     2941 gcaagaagcg gtcaatcccg ggtacctgtc gcgcatgcag cggcgagcgc agaaggactg
     3001 ggggcagggc tactggtggg cgccgggcgc ggccctgccg gacagtgcgc cggacttcgg
     3061 ccacgcagtg gggaggtgaa gcaatgagga cggaccagac caaacggctc gccgaactcg
     3121 ccgagcagct cgtcgacgcg ttcatcaccg aagccgaccc cgcgacctgg ccgggcgccg
     3181 ggcacgagag cgacatgacg cagcgcgagc gcggtgaccg ctactggagc aagaagaacg
     3241 cgtcggccac cggcggcgtc ctgcgcttcg cgctcgacct catcgggcgt accggcgccg
     3301 acgacccgtc cgcgggcggg gacgaagagg acgatctgga caagcgcatc aggcaagccg
     3361 agcgccgcgc cagcgacgcc ctcgcgcgcg cgatgaagcg cgcgtcggcc cccggcgatg
     3421 gccgcgcgac gcgcaccggt tgacttcgcc acgttcttcg cgctgtgggc cgaggaacag
     3481 gggtgggacg tgccggacgt gcactggcgc gcggtgcact ggctgcagca ccgcggtgcg
     3541 cttgccgttc tgttgtgctt tcggggcttc ggcaagagca cgctgctggc cgtgtacaac
     3601 gcctggcgct actacgccga cccgtcgtat cgcatcctgc accagggcga ccaggaccgc
     3661 accgcctaca agaccagccg agacacgcgc tcggtcctgc aacgccaccc gctcacgcgc
     3721 gcgcagccgg ccctcgcgct gcgcggcgag caatcgttct ggtgggtggc cgcgtccgcc
     3781 gacgagcgca acccgtcgat gcaggccgcc ggcatcacga gcaacatcac gagctcgcgc
     3841 tgcgacgagg cgcagaacga cgacgtggag gtgccccgca acatccagaa ccccgaggcg
     3901 cgcgagaaga tgcgctatcg gctgggcgag cagacgcact gcatggtgcc aggcgcccgg
     3961 cagctcttcg tcggcacgcc gcacacgcac gacagcctgt acgacgagat gacgcgcgcc
     4021 ggcgccgacc ggctcgccat cccaatgttc gcgcgcgaga agcgcttcga gtcggtcggc
     4081 gccggcgtgc tcgaggtcgg cttcgtgccg gagctggtct tctcgggtat cggcgccgag
     4141 gcgcggctgc tgacgcgcgg caaggactac gcgctgagcg gtagctcgct cacgctgtcg
     4201 gcgccggccg cgctgctcga ctgctacgcc ggcagcgcct ggccggagcg cttcgggtcg
     4261 aaggagatgc tcgcgcgccg gcgcaagtgc aagaccgtca acgagtggga cagccagtac
     4321 cagctccaca gccggccgct gcacgagatg cgcctcgatc cggccaggct caggcgctac
     4381 agcgcggagc cggtggtgcg ggtggcaaac ggcgagctcg gcatgtggct gggcgacgcg
     4441 cgcatcgtcg ccgcggccgc gcgctgggac ccgtcgagcg gcaaggtcac gagcgacgcg
     4501 tcgtcgctgt cggtgatgct gcaggacgag atcggacgcc gctactggca ccgggtgctg
     4561 gaaatgcgcg gcgaggtcgc gcagaccagc gaggacggca agacgatcat cggcggccag
     4621 gtcacggaga tcgtgcgcgt ggtgcgcgag ctgcacctgc cgcgcgtgag cgtggagacc
     4681 aacggcatcg gcggcttcgc cccggcgttc ctcaaggccg cgttcaagca agcgcggctc
     4741 gtgtgcggcg tgtccgaagt gcagtcgacc gcgaacaaga accggcgcat cctcgaagcg
     4801 ttcgaggggc cgctcaagag tggtcagctc tgggcgcacg actcggtgct cgacgggccg
     4861 acgtggaacc agatgcgcga ctggaatccg gtcgtcccga gccagccaga tgaccacctc
     4921 gacagcggcg ccggggcgct ggtggagacg cccgagcgca tcacgcgcgc cgtcgagcgc
     4981 gccggctccg cgggcttgaa cgcgcccgcg gtgccgtctg aaaattggcg accgtcggcg
     5041 ggcatgcacg aagtcgagct ggagttctga gtctcctgcg gcgcgcccgc gagccaccgc
     5101 gaggcgccca tggcagtcac cgaacagacc ccgatcagca cctacacggc caacggggtc
     5161 gcgacagcct tcgcgttcgc gttccttgcg ttcgaagcgg gctcgctgct ggtgcagacc
     5221 acggacctca ccggcgtcac cgtggacgcg gtgctcggcg tcgactacac ggtcacggga
     5281 ctgggcaacc cggccggcgg cagcgtgacc ttcagcgtcg cgccggcgaa cggataccgg
     5341 gtcacggtga gccgtcacag cggactgcac cgcgacacgg attaccagga caacggcgac
     5401 ttgctgtcgg gcacggtcaa catcgacttc gaccgcctgt ggctcgtgct gcaggagatc
     5461 taccgcggcg gcgggggtct ggcgtcggcg ctgcgcgtgc cgatcggcga agtcgtggcg
     5521 gcgctgccga acgcggccgc acgcgccaac aagacgcttg gcttcgacgc gctgggcgcg
     5581 ccgctgctga tgctgggcgg tgggggtggc ggtggtgggg gcggggatgt cgcggcactg
     5641 caggccgccc tcgccgacgc cgcgaacgtg gccaacggcg acgcgatgtt ggcagtcaag
     5701 cagccgttcg cggggagtgt cgcgcgcacg cagcacgaca agaacatcga caccggcctg
     5761 taccccgagg acttcaggct gccggccgac cccaacgata cgctgtcgat ccaacgcatc
     5821 cttgacctgg gccagttctg ctacctgcgc cccggcaaga cttacgtggc ctacgggttg
     5881 aagtccaaag ccggtacggg gctggtgtgc ctgggcgggc gcgcgacgat cgacgtgccg
     5941 ctcgcggctg gaaacacgtc ggggatatgg atcgacaccg gggatttcat ccttgacggg
     6001 gtgaacttca tcgggcacga gacgggcccg tggaacgtca ccactccaac cggcatggcc
     6061 aatcgcttcg gcgtgatcgt cggcaagctc ttcggcaccg gctcgggagt ggacgggatc
     6121 acggtgcgca attgcgacat cagcggcttc aacaactccg gcctgcaggg cggggaggtg
     6181 caggtcggct acaacttcgg caagcactgc acggtcgaca acgtgtactg cttcgacaac
     6241 tacgtcaact tctggtacgg gccgcgcttc gagtacgtga ccacgacgaa ctgcagcggc
     6301 taccgcggct gggccggcat catcatgcag gccgggaaca acaaattcag cgcctgtcag
     6361 ttcgagaaga acaacgagaa ctgccaactg ccggcgggcg agaacgacgg gcatggcacc
     6421 ttcgtcgggt gctcgttcaa ccactcttgg gccggggcgg gcccaggggg ctacggcctg
     6481 cacgcggcca gcatcgccaa cgggcacgtg ttcatcggct gccagtggtg gttcgcgccg
     6541 attttgctcg acacgtgcac cgggatacgg atcagccaca gtgagatcgt cagctcgccg
     6601 gtgaccatcg tcggcggcgg cctgaacatg atcgatgaca actacgtgcg cgattcgctg
     6661 tcgcgcacgt tcagcggcaa cacgttcacg agcttcaggc gcaaccgcac caagagcacc
     6721 gacaacaccg tcgacgaggc gttcggcgac gtgtacgtgc ggcacaaggc gctaactgca
     6781 gggttcccgt tcgcgctcac cacggggggc tcggcgaaga aaataccttt cagcgcgtac
     6841 tggaacaagt acaacgggct cgatgccgcg ttcctcaccc cggcgaacgg ggacgtctac
     6901 atcccccgtc cgggcaggta tcgggtggcg ctgaacatcg tgctgaaccc cggagcgagc
     6961 ggcgttgtga acctgctcat ccgccggagc ctgtcgggtg gggggtcaga ggatgtactc
     7021 aacgtggccg agacgctggc cgcgcagcgc agcttctgcc tggccgacac gctgctctgc
     7081 gagaacgggg acacgctgtc ggcgatgtac tacgtcgctt ctgcgacgaa cgtggtgtcg
     7141 gcgaccttct cggtgtcttc ggtcgactga aggggcgcgc catgacgatt cgcttcatcc
     7201 ccgccgacaa gtgcgtgcac gccgtagtcg gcgcgctcag cggtgccctg gcgctcgctg
     7261 ctgccgcggc gctgaccatc ggcggcgcgg tgcaggcgct gttcgaggtg gccgacaacc
     7321 tgcgcgccga catctaggcg gcgacggtgg ccgaggtcga cgctgtcaag tggcccgatt
     7381 gagcgcgcac aacatgccag gcgataccga cgatgttccg ctcgacggcg accgagacct
     7441 cgacgcgcgc gagcggcagg tgctgcgcaa gatgatccgc gaccaggagc gcatgcgctg
     7501 gctgtggcgc gtgagccgcg tcggcgtagc gctgttggcc ggcctcgcga ccgcgatcct
     7561 ttggacgatg gatcacgtca agctgatcaa gtaggaggca caacatgcaa ctgccgatga
     7621 tccctcaaga caaggccaac cacgccatct atggaggcgc catcgcactc gtggcggcgg
     7681 caatcgccca cgtagcaggc gcgcgcgagt ggcaggcgca gatcgcgctg ggcgccgcgt
     7741 gcgcagccgg cgtcgccaag gaagtcgccg actacatcgt ggctcgccga cagcgcgcga
     7801 acggggatgc cgtgacgcat ggcgtcgacg ggtgggatgc tgcggtcacg gcgctcggcg
     7861 gcatggcggt cgcaatcgga gtgtggttgt gagcgtgata gatcaggcgc gcccgctgct
     7921 ctctggactg ctgcgccagt gggaagggct gcgcctgtcg ccgtaccgct gcccggccgg
     7981 cgtcccgacg atcggtgtcg gtgcgacgac gtacctcgac gggcggccgg tcacgatggc
     8041 cgaccagccg atcaccgagg agcaggcgct gcgcatgctc gacgtggagt gcgaccgcta
     8101 cctgcaggcg gtgatggaca tgtgcaccgt ggctccgagc gggaacgaac tggccgcgct
     8161 cgcgggcctc gcctacaaca tcgggctcga cgcgctgcgc acgagcacgg ccctgcgcca
     8221 gcacaacgcg ggcaaccacg aggctgccgg ccgcgccatc gccctgtggg acaaggcgcg
     8281 cgttggcggc acgctgacgg tgctgccggg cctgacggcg cgcagggcgg ccgagcgggc
     8341 gctgtacctc acaccagcca gcggcgaggt cgagcgcatg ccgcaggccg tcgagagcga
     8401 gtcgccgatg gcagcgagcc cgatcaaccg cggcggcgca gccgttgtcg gcctgggcgc
     8461 gatcgagggc ctgcgccagg ccggcgactc gatcgccgtc gtgcgcgctc cgctcgatgc
     8521 actcaagggc ctgctgacgg acaccatcgg cctgccgcca gagtggctgc tgccggtcgc
     8581 gctcgcggcg gtcggcgcct acgtcgtcta ctggcggctg cagcagcgcc ggcagggctt
     8641 tgcgtgacat ggtggcgctg ctgcgcgtcg tgccgatctg ggcctgggcg ctcgtcgcag
     8701 cgctcgtctg gggcgcgtgg cagcgtcacg aggcgcggca gcagggcgcc ctgcgcgcgg
     8761 ccgatgccgc tgccg
//
LOCUS       DAOQNN010000102         8655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4049_length_8655_cov_8.664186, whole genome shotgun sequence.
ACCESSION   DAOQNN010000102 DAOQNN010000000
VERSION     DAOQNN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4049_length_8655_cov_8.664186"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKV97_07430"
     CDS             complement(<1..222)
                     /locus_tag="PKV97_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase"
                     /protein_id="HNB05745.1"
                     /translation="MFQHSAPSPAWLQHDMERVDAIIRAHMNNDVALIRQVVEFILHG
                     AERRRPALVLASARALGHGGERPHALAAAV"
     gene            425..1765
                     /locus_tag="PKV97_07435"
     CDS             425..1765
                     /locus_tag="PKV97_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004387139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNB05746.1"
                     /translation="MKNIKLALLLVLATLSLLWWWADPLLVQGNTYFALRTLAVNYTG
                     VLAMGAMSIAMILALRPAALEPLLGGMDKAYRLHKWLGIAGLSVGVVHWLWAKGTKWA
                     VGWGWLQKPARGPRPEQTVELFRFFQEQRGLAETIGEWAFYLFAVLAAIALIKRFPYR
                     RFVQTHRVMAAVYLALVVHGVVLLPFAYWTQPLGMVMGMLMAAGSWAALRSLAGRIGR
                     GRRAHGRIERVVRHPANQVLEVGLRLDTPWPGHQAGQFAFVTFDEREGPHPFTISSAW
                     GGDGRMDFMIKPLGDYTRSLPATLKLGDPVRVEGPYGRFDFGGAQARQIWVAGGIGIT
                     PFVARMQARAQEDRQGGRAAAIDLFYSTSAPDEGFIARLRELAARARVQLHVLVSPRD
                     GRLDADGICRSVPDWKQAGVWFCGPAGFGAMLRDTLAARGLPAERFHQELFEMR"
     gene            1973..2806
                     /locus_tag="PKV97_07440"
     CDS             1973..2806
                     /locus_tag="PKV97_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HNB05747.1"
                     /translation="MNDALAIPAAFAERRLFEPIAPWLARFTTPGLPSRAALDALLAE
                     AAPAACSGGGAPIRFAAPGDDEAGYEARIFATGEVPTREADWHDFFNALAWCAWPRTK
                     ACCNRLHLDELRARDAAGLAGRGPRRDALTQFDECGIVVVSADPQVPALLAAHAWEEV
                     FWNRREALQHSTRFLVFGHGTWDQLRAPFPGLCAKAIHRVVDPAWLALPAAAALAETD
                     AWLAARLASTITDFSPRSLAPLPLLGIPGVTPASESAGYYRDTRQFRPQRMPAGTAAG
                     A"
     gene            3005..3901
                     /locus_tag="PKV97_07445"
     CDS             3005..3901
                     /locus_tag="PKV97_07445"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HNB05748.1"
                     /translation="MRPPTRIPAPALRRPGSCICCLILALALLPAGAVAQTAEPADAP
                     AAMLALRYDPAIDPAGWWVSEKLDGVRASWDGSVLRFRSGRALPAPAWFLAALPPVAL
                     DGELWLGRRRFDELSGLLRREDPADPLWREVRYMLFELPGAAGDFDTRLARLRSLAEA
                     ADAPWVQAIPQQRVPDRAALQRLLGEILAGGGEGLMLHRADALWQPGRSEALRKLTPW
                     LDAEARVVGYLPGKGRLQGRVGALQVERADGRRFRIGSGLSDALRANPPALGALVTYR
                     YRELTPAGLPRFPRYLRERTLP"
     assembly_gap    4037..4136
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4258..6189)
                     /locus_tag="PKV97_07450"
     CDS             complement(4258..6189)
                     /locus_tag="PKV97_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNB05749.1"
                     /translation="MPSDTASPAPLSALRGLLPFLRPYRARVVLAFVFLCLGSAAILV
                     VPLAFRDLIDAGFGGVSVGGAATGGAADRGGLFGAQGLDGRFVALFGLAVFWAAAVAA
                     RYYTVSWIGERVTADLRSAVYARVLRQSPQYFETLQTGEVLSRLTGDTTLVQTVVGSS
                     ISLGLRSLFQFVGGMLMLAVTSVQLFGLIFGLMALLALPITLIGRRVRSLSREAQDRI
                     ADASALAGEILAAMPTVQAFGQEDNEAARFVARAETSFAAAVRRTRVRAALTGLIIAA
                     VMGTIIFVLWIGARQVQAGALSVGDLGAFVIYAALVAGGAGTLAEVWGDVMRAAGASG
                     RLLELLQAEPVIRDAEAAEGVAEAAGGGGSRRAPRLHENPRSTRPAGAAPAANPAPDS
                     PTKSPATHPAIELADVVFRYPARPAVPALDGIDLRIAVGERLALVGPSGAGKTSLFQL
                     LLRFYEAGEGCIRIDGHDIRALPLRSLRARIALVPQDPVIFSASALDNIRYGRPDAGD
                     AEVIAAARAAAADEFIARLPEGYATFLGERGTRLSGGQRQRIAIARAILKGAPILLLD
                     EATSALDAESEVLVQQGLAAAMQGRTTLIIAHRLATVQKVDRIVVMDGGRIFETGTPA
                     DLRRQGGLYARLAALQLEG"
     gene            6397..7005
                     /locus_tag="PKV97_07455"
     CDS             6397..7005
                     /locus_tag="PKV97_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HNB05750.1"
                     /translation="MDTPATIHAFWFGPAADADAEAAIIERQSALWWRKQPAVDAEIR
                     ARFAPLVGRAAGGELDTWLGGLRGRLALVLLTDQFPRNIWRGEAAAFAFDVLALRWAK
                     EALARGLDAGLRPIERVFLYLPLEHSENLADQREAVRRFDTLAIAVEPALRTAFAGYL
                     DYARRHLEIIERFGRFPHRNAALGRETSPEEAEFLRQPGSRF"
     gene            7044..7748
                     /locus_tag="PKV97_07460"
     CDS             7044..7748
                     /locus_tag="PKV97_07460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HNB05751.1"
                     /translation="MKAQILQHVPFEGPGHIGDWLAARGADIGSTRFWESPVLPDPAA
                     VDLLVVMGGPMSVNDEAEFPWLVAEKRFIAEVVALGRPVLGICLGAQLIASAHGARVY
                     RNADKEIGWFEVAASGAEPDAADTASRFPFPARTTVLHWHGETFDLPAGARLLASSPA
                     CRHQAFQLGGRAIGLQFHLEMTASSLRAIVDNCRGELVPGRWIQDEATILGHADGHFA
                     ATHALMDGLLDWLVRA"
     gene            complement(7756..>8655)
                     /locus_tag="PKV97_07465"
     CDS             complement(7756..>8655)
                     /locus_tag="PKV97_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNB05752.1"
                     /translation="DTMNLKPVGTSSLVNLEGRLGVFTPVAEDGSGADNTEAVIAALR
                     KELVRLESENAALRRQLAEVEAARKRSPDDFASAVAHTLDTLQHKLAQTTNPVSRFAV
                     REVAIDAQVTVDVSPLGTLEYRFVRPEDDIDPSKLSRIRLDLVPLPREDAGLSASPEF
                     SPWAGVEDIVGVGETWRGALNRQQIFTIADLLVAGSRLKSSAELAALVGVDRQRLAEW
                     MGHAELLSVRTIEPRAADVLWTVGIRSLETLAGAAAVEVVEKYNAEVARRAHKSLAPI
                     NAAQAASWIEAAKKYHGRPREGG"
BASE COUNT         1136 a         3261 c         2997 g         1161 t
ORIGIN      
        1 caccgccgcc gccagcgcgt gcgggcgctc gccgccgtga ccgagggcgc gcgcgctcgc
       61 cagaaccagc gccggccgcc ggcgctcggc accgtgcagg atgaactcga cgacctggcg
      121 gatcagcgcc acgtcgttgt tcatgtgcgc gcggatgatc gcgtcgaccc gctccatgtc
      181 gtgctggagc caggcggggg agggggcgga gtgctggaac atgttcgggg tgccgtgcgg
      241 tggccgcaaa cggcggccgg tcgaggttcg cccgcgagtc tataggcgcg ccgccacgct
      301 gaccagccgc aggcgcccgc gcgggctaca agccggtaca aggcggacac atgctgtggc
      361 gcacaatttc ccgcatggga tccgctgcct catggcggcc gcattccgct cgaagggagc
      421 catcatgaag aacatcaaac tcgcactcct cctcgtcctc gccacgctca gcctgctgtg
      481 gtggtgggcc gatccgctgc tcgtccaggg caacacctac ttcgcgctgc gcacgctcgc
      541 ggtcaactac accggcgtgc tcgccatggg cgcgatgagc atcgcgatga tcctcgccct
      601 gcgcccggcc gcgctcgagc cgctgctcgg cggcatggac aaggcctacc ggctgcacaa
      661 gtggctgggc atcgccggcc tgtcggtcgg cgtggtgcac tggctgtggg ccaagggcac
      721 caagtgggcg gtcggctggg gctggctgca gaaacccgcg cgcggcccgc gccccgagca
      781 gaccgtcgag ctcttccgct tcttccagga gcagcgcggc ctcgccgaga ccatcggcga
      841 gtgggccttc tacctcttcg ccgtgctcgc cgcgatcgcg ctgatcaagc gcttccccta
      901 ccgccgcttc gtacagaccc accgcgtgat ggcggcggtg tacctcgcac tggtcgtgca
      961 tggcgtggtg ctgctgccct tcgcctactg gacgcagccg ctcggcatgg tcatgggcat
     1021 gctgatggcc gccggcagct gggccgcgct gcgctcgctc gccggccgca tcggccgcgg
     1081 gcgccgtgcg cacggccgca tcgagcgcgt ggtgcggcac cccgccaacc aggtgctcga
     1141 ggtcggcctg cgcctcgaca cgccttggcc gggacaccag gccggacagt tcgccttcgt
     1201 caccttcgac gagcgcgagg gtccgcaccc cttcacgatc tcctccgcct ggggcggcga
     1261 cggccgcatg gatttcatga tcaagccgct cggcgactac acccgcagcc tgccggcgac
     1321 gctgaagctg ggtgacccgg tgcgcgtcga aggcccctac ggccgcttcg acttcggcgg
     1381 cgcccaagct cggcagatct gggtcgccgg cggcatcggc atcaccccct tcgtcgcccg
     1441 catgcaggcg cgtgcgcagg aagacaggca gggtgggcgc gcggcggcga tcgacctctt
     1501 ctacagcacc agcgcccccg acgaaggctt catcgcccgc ctgcgcgaac tcgccgcgcg
     1561 ggcgcgggtg cagctgcacg tgctggtctc gccacgtgac ggccgcctcg acgccgacgg
     1621 catctgccgc agcgtgcccg actggaagca ggccggcgtg tggttctgcg gcccggccgg
     1681 cttcggcgcg atgctgcgcg acaccctcgc cgcccgcggc ctgcccgccg agcgcttcca
     1741 ccaggagctg ttcgagatgc gctgaggcgg atgatccgga gtcccgggca acttccaagg
     1801 gtttggcgca ggcgcgacgc aagtcgcgat tcgagcgctc acgcgacgcg acacgtcgtc
     1861 tcctgcgaat ggcgcggcaa tcgcgacctg cctcgctcct gccaaaggct cgaaaccccg
     1921 accgcggcac gcaccctggc tgcaccgccc gcgagccggc acaattccac agatgaacga
     1981 cgccctcgcc atccccgccg ccttcgccga gcgccggctg ttcgagccga tcgcaccgtg
     2041 gctggcccgc ttcaccacac ccgggctgcc ctcgcgcgcc gcgctcgacg ccctgctcgc
     2101 cgaggccgcg cccgcggcgt gcagcggtgg cggcgcgccg atccgcttcg ccgcgccggg
     2161 cgacgacgag gccggctacg aggcgcgcat cttcgccacc ggcgaggtcc ccacccgcga
     2221 ggccgactgg cacgacttct tcaacgcgct cgcctggtgc gcctggccgc gcaccaaggc
     2281 ctgctgcaac cgcctccacc tcgacgagct gcgcgcacgc gatgccgccg gcctcgccgg
     2341 acgcgggccg cggcgcgacg cgctgaccca gttcgacgag tgcggcatcg tcgtggtgtc
     2401 cgccgacccg caggtccccg ccctgctcgc cgcgcacgcg tgggaagagg tgttctggaa
     2461 ccggcgcgag gccctgcagc acagcacccg cttcctcgtc ttcggccacg gcacctggga
     2521 ccagctgcgc gcgcccttcc ccggcctgtg cgccaaggcc atccaccgcg tggtcgatcc
     2581 ggcctggctc gcactgcccg cggccgcggc gctcgccgag accgatgcct ggctcgctgc
     2641 ccggctcgcg agcaccatca ccgacttcag cccgcgcagc ctcgccccgc tgccactgct
     2701 gggcatcccc ggcgtgacgc cggccagcga gtccgccggc tactaccgcg acacccgcca
     2761 gttccgcccg cagcgcatgc ctgcgggcac cgccgccggc gcttgaactt ccgcctggcg
     2821 gcgcccacgc accgcagcta caatcctttt gtcattttcc ggcctcgcat gccgacgtgc
     2881 tcgaggcgag catcgagggc acaggccgcc gccccgcgag gggcttgccc gcaagccttg
     2941 tccgcgttgg cgcggcacgg gcgaagaggt gcccacgccc gcgatcggac tggagggttc
     3001 gcccgtgaga ccgcccaccc gcattcccgc accggccctg cgcaggccgg gcagctgcat
     3061 ctgctgcctg atcctggccc tcgccctgct gccggccggc gcggtcgcac agaccgcgga
     3121 gcccgcagac gcgcccgcag ccatgctcgc gctgcgctac gaccccgcga tcgaccccgc
     3181 gggctggtgg gtgagcgaga agctcgacgg cgtgcgtgcg tcgtgggacg gcagcgtgct
     3241 gcgtttccgc agcgggcgcg cgttgccggc gccggcctgg ttcctcgccg cgctgccgcc
     3301 ggtcgccctc gacggcgagc tctggctcgg ccgccgccgc ttcgacgagc tctccggcct
     3361 gctgcgccgc gaggatcccg cagacccgct gtggcgcgag gtgcgctaca tgctcttcga
     3421 attgcccggc gccgccggcg acttcgacac ccggctggcg cgtctgcgca gcctggccga
     3481 ggccgccgat gcgccctggg tgcaggcgat cccgcagcag cgcgtacccg accgtgccgc
     3541 cttgcagcgc ctgctcggcg agatcctcgc aggtggtggc gaaggcctca tgctgcatcg
     3601 cgccgacgcc ctctggcagc ccggccgcag cgaggccctg cgcaagctca ccccctggct
     3661 cgacgccgag gcgcgcgtgg tgggctacct gccgggcaaa ggcaggctgc agggcagggt
     3721 gggcgcgctg caggtcgagc gcgcggacgg gcggcgcttc cgcatcggca gcggcctcag
     3781 cgacgcgctg cgcgccaacc cgcccgcgct cggcgccctc gtcacctatc gctaccgcga
     3841 gctcaccccc gccggcctgc cgcgctttcc gcgctacctg cgcgaacgca cgctgccgtg
     3901 aagcgccggc gccatccggg agcgtcagcg cgtgcgtgcc cgccgcaggg cgcaacggat
     3961 ccgcgcctgc gccacagggc cgcccgatcg cgacttgcgt cgctcccaca aggctcgccg
     4021 aagcgcggcg gttttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccc
     4141 gatcgcgact tgcgtcgctc ccacaaggct cgccgaagcg cggcggtttt ccgtgggagc
     4201 gacgcaagtc gcgattgcag cgcaccgggt ccgcgcctgc gctgcgggag gctccggtca
     4261 gccctccagc tgcagcgccg ccaaccgcgc atacagccca ccctggcggc gcaggtcggc
     4321 cggggtgccg gtctcgaaga tgcggccgcc gtccatcacc acgatgcgat ccaccttctg
     4381 caccgtcgcg aggcggtggg cgatgatcag cgtggtgcgg ccctgcatcg cggcggccag
     4441 gccttgctgc accaggacct cggattcggc gtcgagcgcg ctggtggcct cgtcgagcag
     4501 caggatgggg gcgcccttga ggatcgcgcg cgcaatcgcg atgcgctggc gctggccgcc
     4561 cgagagccgc gtgccgcgct caccgaggaa ggttgcgtag ccctcgggca ggcgggcgat
     4621 gaactcgtca gcggccgcgg cgcgcgcggc ggcgatcact tcggcgtcgc ctgcgtcggg
     4681 gcgaccgtag cggatgttgt cgagcgcgct cgcggagaag atcaccgggt cctggggcac
     4741 gagggcgata cgtgcgcgca ggctgcgcag cggcagcgcg cggatgtcgt ggccgtcgat
     4801 gcggatgcag ccctcgccgg cctcgtagaa gcgcagcagg agctggaaca ggctggtctt
     4861 gccggcaccc gaggggccga ccagcgcgag cctttcgccc accgcgatgc gcaggtcgat
     4921 gccgtcgagc gccgggacgg cgggtctggc agggtagcgg aagacgacgt cggcgagttc
     4981 gatcgcgggg tgggtggcag gggacttggt gggggaatcg ggtgcggggt tcgcggcggg
     5041 cgccgcgccc gcagggcgcg tgctgcgcgg gttctcatgc aggcgcggcg cccgtcgcga
     5101 accgccacct ccggccgcct ccgctacgcc ctccgccgcc tctgcatccc ggatcaccgg
     5161 ctctgcctgc agcagctcca gcagccgccc gctcgccccc gcggcgcgca tcacgtcgcc
     5221 ccacacctcg gcaagcgtgc cggcgccgcc cgcgaccagc gcggcgtaga tcacgaaggc
     5281 gccgagatcg cccaccgaca gcgcgccggc ctgcacctgg cgcgcgccga tccacagcac
     5341 gaagatgatc gtgcccatca ccgcggcgat gatcagcccg gtgagcgccg cgcgcacccg
     5401 ggtgcgccgc accgcggcgg cgaagctggt ctcggcgcgg gcgacgaaac gcgcggcctc
     5461 gttgtcctcc tgcccgaagg cctgcaccgt gggcatggcc gcgaggatct cgccggcgag
     5521 cgccgaggcg tcggcgatgc ggtcctgcgc ctcgcgcgag aggctgcgca cgcgccggcc
     5581 gatcagcgtg atgggcagcg cgagcagggc catgagcccg aagatgagcc cgaagagctg
     5641 cacgctggtc accgccagca tcaacatgcc gccgacgaac tggaagaggc tgcgcagccc
     5701 cagcgagatc gagctgccca ccaccgtctg caccagcgtc gtgtcgccgg tgagccgcga
     5761 cagcacctcg ccggtctgca gggtctcgaa gtactgcggc gactggcgca gcacgcgcgc
     5821 atacaccgcg ctgcgcaggt cggcggtgac gcgctcgccg atccacgaca ccgtgtagta
     5881 gcgcgccgcc accgccgccg cccagaacac tgcgaggccg aacagcgcga cgaaacgtcc
     5941 gtccagcccc tgcgcgccga acaacccgcc gcggtccgcc gccccgcccg tcgcggcacc
     6001 gccgacggac acgccgccga agcccgcgtc gatcaggtcg cgaaaggcca gcggcaccac
     6061 caggatggcc gccgagccca ggcacaagaa cacgaaggcg agcaccaccc gtgcgcggta
     6121 ggggcgcagg aaggggagca ggccacgcag ggcggagagc ggggcggggg aggcggtgtc
     6181 gctgggcatg gcggcattat tgcgccaccc gcgcggacgg ggtgcgaacc aggcgtccgg
     6241 agcagggcga ttgcatgaag gcgtggcagg cgctcatcgc ctgccgcgtg ggagcggaac
     6301 agtccgttgg agcgatcgcc ctcgcgcccg acgctcccgc atcgcagcgg gctgggctat
     6361 gcttgcccca ccccgatcgg ccgcccggag ccccgcatgg acacgcccgc cacgatccac
     6421 gccttctggt tcggccccgc ggccgatgcg gacgcagagg ccgccatcat cgagcgccag
     6481 tcggcgctat ggtggaggaa acagcccgcg gtcgacgcgg agatccgcgc acgcttcgcc
     6541 ccgctggtgg gccgcgccgc gggcggggag ctcgacacct ggctgggcgg gctgcgcggg
     6601 cgcctggcgc tggtgctgct gaccgaccag ttcccgcgca acatctggcg cggcgaagcc
     6661 gcggccttcg ccttcgacgt gctcgcgctg cgctgggcga aggaggcgct cgcgcgcggc
     6721 ctcgacgccg ggctgcgccc gatcgagcgc gtcttcctgt acctgccgct ggagcactcc
     6781 gaaaacctcg ccgaccagcg cgaggcggtg cgtcggttcg atacgctcgc catcgcggtc
     6841 gagcccgcgc tgcgcacggc cttcgccggc tacctcgact acgcccgccg ccacctcgag
     6901 atcatcgagc gcttcggccg cttcccgcac cgcaacgccg cgctcgggcg cgagacgagc
     6961 ccggaggagg ccgaattcct gcgccagccc ggctcgcgct tctagaatag gccacccgtt
     7021 ccatctcgcc accccaggac gccatgaaag cccaaatcct gcagcacgtt cccttcgaag
     7081 gccccggtca catcggcgac tggctcgccg cgcgcggtgc ggacatcggc tcgacccgct
     7141 tctgggagtc gccggtgctg cccgacccgg ccgcggtcga tctgctggtg gtgatgggcg
     7201 ggccgatgag cgtcaatgac gaggcggaat tcccctggct ggtcgccgag aagcgcttca
     7261 tcgcggaagt ggtcgcgctc ggccggccgg tgctcggcat ctgcctgggc gcgcagctga
     7321 tcgccagcgc gcacggcgcc cgcgtctatc gcaacgcgga caaggagatc ggctggttcg
     7381 aggtcgccgc cagcggagcg gaacccgacg ctgcagacac cgcatcgcgc ttccccttcc
     7441 ccgcgcgcac caccgtgctg cactggcacg gcgagacctt cgatctgccc gcgggcgcgc
     7501 gcctgctcgc gtcgagcccg gcgtgccgcc accaggcctt ccagctcggc gggcgtgcga
     7561 tcggcctgca gttccacctc gagatgaccg catccagcct gcgcgcgatc gtcgacaact
     7621 gccgcggcga gctggtgccg ggacgctgga tccaggacga ggcgacgatc ctcggccacg
     7681 ccgacgggca cttcgccgcg acccacgcgc tgatggacgg cctgctcgac tggctcgtac
     7741 gcgcctgagg cagcttcagc cgccctcgcg cggccggccg tggtacttct tcgcggcctc
     7801 gatccaggag gcggcctgcg ccgcgttgat cggggcgagg ctcttgtgtg cgcggcgggc
     7861 gacctcggcg ttgtatttct cgacgacctc caccgccgcc gcgccggcga gcgtctccag
     7921 cgagcggatg ccgaccgtcc acagcacgtc ggccgcgcgc ggctcgatcg tgcgcaccga
     7981 gagcagctcc gcatgaccca tccactcggc caggcgctgc cgatccaccc ccaccagcgc
     8041 ggccagttcg gcgctcgact tgagccgcga cccggccacc agcaggtcgg cgatggtgaa
     8101 gatctgctgg cggttgagcg cgccgcgcca ggtctcgccg accccgacga tgtcttccac
     8161 gcccgcccac gggctgaact ccgggctggc gctcaggccc gcgtcctcgc gcggcagggg
     8221 cacgaggtcg agccggatgc gcgacagctt ggacggatcg atgtcgtcct cggggcgcac
     8281 gaagcggtac tcgagggtgc ccagcggcga cacgtccacc gtcacctggg cgtcgatcgc
     8341 cacctcgcgc accgcgaagc ggctcaccgg gttggtggtc tgcgccagct tgtgctgcag
     8401 ggtgtccagg gtgtgggcga ccgcgctggc gaagtcgtcc gggctgcgct tgcgcgccgc
     8461 ctcgacctcg gcgagctggc ggcgcagcgc ggcgttctcg ctctcgaggc ggacgagctc
     8521 cttgcgcagc gccgcgatca ccgcctcggt gttgtcggcg ccgctgccgt cctccgccac
     8581 cggggtgaag acgcccagcc tgccctccag attcaccagg ctggacgtgc cgacgggctt
     8641 caggttcatc gtgtc
//
LOCUS       DAOQNN010000103         8629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4066_length_8629_cov_11.228831, whole genome shotgun sequence.
ACCESSION   DAOQNN010000103 DAOQNN010000000
VERSION     DAOQNN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4066_length_8629_cov_11.228831"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PKV97_07470"
     CDS             complement(<1..544)
                     /locus_tag="PKV97_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB05753.1"
                     /translation="MDSSPKPSLLERLSALLSREPEDRDELLALLHSAFDRNLIDADA
                     LTIIEGALQMSDMQVRDVMVPRAQMDCVHLDDPIDTIANFAIDTAHSRFPAIGDGKDD
                     VVGILLAKDLLRYFAGREFDLRDMLRPAVFVPESKRLNVLLREFRVSRNHMAIVVDEY
                     GGVAGLVTIEDVLEQIVGDIE"
     gene            complement(567..1391)
                     /gene="ybeY"
                     /locus_tag="PKV97_07475"
     CDS             complement(567..1391)
                     /gene="ybeY"
                     /locus_tag="PKV97_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNB05754.1"
                     /translation="MANKTNRTDKTNQAAARKSAGTWPRIIAVDADGKAARIRAERLE
                     IELGDGRKLLLAFPEKAWGDLEIEAETDDDNAVPMLSLQPGACNLMTLRVDVHHDMSF
                     VEPLALPAGEHPPVLKLQVQKAVEGEDKANCPKKNHIRRWAAAALRASAQVTVRLVGE
                     AEGRALNRDYRGKDYATNVLTFAYAEGEDLPGLPAAAADVPLAGDLVLCVPVVVREAA
                     AQGKTLEAHFAHLVVHGMLHLQGYDHENETEAAEMEALETAILRELGYADPYALSA"
     gene            complement(1381..2400)
                     /locus_tag="PKV97_07480"
     CDS             complement(1381..2400)
                     /locus_tag="PKV97_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNB05755.1"
                     /translation="MAKILEVFFEPVDNARLAKLCGVLDENLRQIENAFDITVSRRAE
                     HFTLQGHPAQVLRGEMALKHFYALADKDLSRDEVQLGLIEIANKGEAAQPAPVLMTRR
                     TELHGRTPRQVDYLRNIQDFDITFGIGPAGTGKTYLAVASAVDAFERDLVERIILTRP
                     AVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFDRVGKLFERGSIEIAPLAFMRG
                     RTLNNAFIILDEAQNTTPEQMKMFLTRIGFGAKAVVTGDLTQIDLARGQRSGLKEARA
                     VLAGVRGIAFTEFSKEDVVRHPLVARIVEAYDLEAARLEREKAAARAARQQPHPQEQE
                     AEDGE"
     gene            complement(2401..3765)
                     /gene="miaB"
                     /locus_tag="PKV97_07485"
     CDS             complement(2401..3765)
                     /gene="miaB"
                     /locus_tag="PKV97_07485"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNB05756.1"
                     /translation="MKKLYIRTFGCQMNEYDSDKMADVLGAHEELVKTDNPEEADVIL
                     FNTCSVREKAQERVFHDLGRVRLLKQKKPGLIIGVGGCVASQEGEAIVKRAPYVDVVF
                     GPQTLHRLPSLIAARKQSGRSQVDISFPEIEKFDAMPPARVEGASAFVSIMEGCSKYC
                     TFCVVPYTRGDEVSRPLEDVLAEIAGLAAQGVKEVTLLGQNVNAWRGVLSREDGEQGA
                     DVGDFAFLLECVAEIPGIERIRYTTSHPREMTQRLIDCYAKIPKLVSQLHLPVQSGSD
                     RVLAAMKRGYSVLEFKSVVRKLRAARPDLSLTSDFIVGFPGETEEDFEKTMKLIDDVG
                     FDGSFSFVYSSRPGTPAADLEDPVAQETKLAWLARLQKRIDAQYQANSEAMVGTVQRI
                     LVEGAAKKNAEAEMMGRSDNNRVVNFPSDSPVRDRLVGQFVEVRITAALPHSLRGEIL
                     TRES"
     gene            complement(3824..4264)
                     /locus_tag="PKV97_07490"
     CDS             complement(3824..4264)
                     /locus_tag="PKV97_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05757.1"
                     /translation="MERLIYKAFALTSPGRWTIIGALFPTVPDLSGPSLRPHFLSVLL
                     AGLSGLASSVAFAALPRPIPDDAARVKMTFSSASVVQVKDATLQLSPGAQIRDTHNRI
                     VLPSHVSGEYVVRMLVDRNGQVHRVWILTPEEAAAPMPKAPSRP"
     gene            4441..4517
                     /locus_tag="PKV97_07495"
     tRNA            4441..4517
                     /locus_tag="PKV97_07495"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4475..4477,aa:Met,seq:cat)
     gene            4715..6523
                     /locus_tag="PKV97_07500"
     CDS             4715..6523
                     /locus_tag="PKV97_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HNB05758.1"
                     /translation="MSSTHVATLPKQPPRRPPHRDGGRRFLLLAGGYWNCARKWQVRG
                     TVLALALLTMAQVGLAIWVSYWNRDLFDALEERALSELLQLVGVFVLIFALTMGVTAL
                     HMHVKRRLQLDWRRWLTDLLLEHWLSHGHLYRLQFTAGEHDNPDGRIAEDIRIATDVA
                     VSLAHSLLFALLILGSFIDILLRVSGSAPLPGTSIAVHGYMVLAAFLYAGVGAALGLM
                     LGRPLIRATNRLQTVEANLRFGLARAREHAEPIAMMHGEPHERREAARLFDDVGRGWN
                     RQTLAYLGIVSFSTGYGTLLPVFPILIAAPQYIAGAMTLGVLMQAAQAFQRLTTALSW
                     PIDNLGELARCRASIDRVASLYEDMLALERAEARSRGRERIAVCSSPHPELELRRLRL
                     QTPSGEILLDDFDLRVRRGERVLICGDPAVTVSLFKAVAGLWPWGSGEVRLPLGRDLM
                     FVPRQPFLPAGSLRMVLSYPDDADRHADAVLERALECAGIAWLAPKLDTQEDWGRELP
                     LRIQQRLGVARVFLHQPGWIFIEEATDALAPKEEVDTLELLHHELPSSTLLVISRRAR
                     QNHFFERRLLLQRPRPGLQPPAAPAARPETPSLPEA"
     gene            complement(6509..7615)
                     /locus_tag="PKV97_07505"
     CDS             complement(6509..7615)
                     /locus_tag="PKV97_07505"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HNB05759.1"
                     /translation="METTRTKLRGVNLGSWLLLEKWMVPSLFEGLEATDETTWCAELG
                     PAAAERLRRHWASFVTREDFAWIAARGLNAVRIPIGHWIFGPDYPYHPKYGAHRHPFV
                     TGGIEVLDRALDWAQEFGLRVIIDLHAAPGCQNGFDNGGIKDVVEWHTKKEYLEHSLA
                     VLERLAERYRAHPALHGIELLNEPRWDVPTDYLKSYYLEAYARIRKHCAPETVAVVFH
                     DGFRSFREYLGFMQAPAFRNVVFDYHRYQCFERGDIDMDIHGHIRKAAVDWREEADAI
                     NAELGLPAVCGEWSLGLDLKVVSLWAEGPFNHALEHMDDFQQDVASRAYGDSQLMTFE
                     RLAGWFFWSYKTETTPAWCLRACVERGWLPSRFG"
     gene            7863..>8629
                     /locus_tag="PKV97_07510"
     CDS             7863..>8629
                     /locus_tag="PKV97_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HNB05760.1"
                     /translation="MRTVEHVVTHLILHALVREPGAPSRLMLREKPCALDAAALRLVE
                     RLCAQYESRSAKGFGRLEEDEESYPLARWLREHLVDETLDFTALSRQLAERVQAIADE
                     EELEEGGHLVLARIREGEGDSLWVALLGSSDGVAIGGDLAPLDCTHVDFSALHAAGRV
                     DLAGWRRGDERYLGFLRGRGKGGAWFKRVLGCSDVMVALQETKKLVATLDRFVDTERL
                     APATRDAVLERAHDYLDTLGEAGAPVVFEELAREVFP"
BASE COUNT         1270 a         2994 c         2974 g         1391 t
ORIGIN      
        1 cctcgatgtc gccgacgatc tgctcgagca cgtcctcgat ggtgaccagg ccggccacgc
       61 cgccgtactc gtcgaccacg atcgccatgt ggttgcgcga cacgcggaac tcgcgcagca
      121 gcacgttgag acgcttggac tcgggcacga agactgcggg gcgcagcatg tcgcgcaggt
      181 cgaactcgcg cccggcgaag tagcgcagca ggtccttggc gagcaggatg ccgacgacgt
      241 cgtccttgcc gtcgccgatc gcggggaagc gcgagtgggc ggtgtcgatc gcgaagttgg
      301 cgatcgtgtc gatcgggtcg tccagatgca cgcagtccat ctgggcgcgc gggaccatca
      361 cgtcgcgcac ctgcatgtcg gacatctgca gggcgccttc gatgatggtg agggcgtcgg
      421 cgtcgatcag gttgcggtcg aaggccgagt gcagcagcgc gaggagttca tcgcgatctt
      481 ccggttcgcg cgagagaagg gccgaaagtc gctcgagtaa ggagggtttg ggggaactgt
      541 ccattttggg ggtcggtgtc tccgcttcag gcgctcaagg cgtaggggtc ggcatagccg
      601 agctcgcgca ggatcgcggt ctcgagcgct tccatctcgg ccgcctcggt ctcgttctcg
      661 tgatcgtaac cctgcaggtg cagcatgcca tgcacgacga ggtgggcgaa gtgcgcctcc
      721 agggtcttgc cctgggcggc ggcctcgcgc accaccaccg gaacgcacag caccaggtcg
      781 ccggcgagcg gcacgtctgc ggccgccgcg ggcaggccgg gcaggtcctc gccctcggcg
      841 taggcgaagg tcagcacgtt ggtggcgtag tccttgccgc ggtagtcgcg gttgagtgcg
      901 cgcccctcgg cctcgcccac caggcgcacc gtgacctggg cgctggcgcg cagcgccgcc
      961 gcggcccagc gccggatgtg gttcttcttg gggcagttgg ccttgtcctc gccctcgacc
     1021 gccttctgca cctgcagctt cagcaccggc ggatgctcgc ccgcgggcag cgcgagcggc
     1081 tcgacgaagc tcatgtcgtg gtgcacatcc acgcgcaggg tcatcaggtt gcaggcaccg
     1141 ggctgcagcg acagcatcgg caccgcgttg tcgtcgtcgg tctcggcctc gatctcgagg
     1201 tcgccccagg ccttctcggg gaaggcgagc agcagcttgc gaccgtcgcc gagctcgatc
     1261 tccaggcgct cggcgcggat gcgcgcagcc ttgccgtcgg catcgacggc gatgatcctg
     1321 ggccaggtgc cggccgactt gcgcgcggcc gcctggttcg tcttgtcggt cctgttggtc
     1381 ttattcgcca tcttcggctt cctgctcctg cgggtgcggc tgctggcgcg cggcgcgggc
     1441 cgcggccttc tcgcgctcga ggcgcgcagc ctcgaggtcg taagcttcga cgatgcgcgc
     1501 gacaagcgga tgacgcacca cgtcttcctt gctgaattcg gtgaacgcga tgccgcgtac
     1561 ccccgccagc accgcgcgcg cctccttgag accgctgcgc tggccgcgtg ccaggtcgat
     1621 ctgggtgagg tcgccggtga ccaccgcctt ggcgccgaag ccgatgcggg tgaggaacat
     1681 cttcatctgc tcgggggtcg tgttctgcgc ctcgtcgagg atgatgaagg cattgttgag
     1741 cgtgcgcccg cgcatgaagg cgagcggcgc gatctcgatg ctgccgcgct cgaagagctt
     1801 gccgacgcgg tcgaagccca tcaggtcgta gagcgcgtcg tagagcgggc gcaggtaggg
     1861 gtcgaccttc tgcgccaggt cgccgggcag gaagcccagg cgctcgccgg cctcgaccgc
     1921 cgggcgggtg aggatgatgc gctcgacgag gtcgcgctcg aaggcgtcga ccgcgctcgc
     1981 caccgcgaga taggtcttgc ccgtgccggc cgggccgatg ccgaaggtga tgtcgaagtc
     2041 ctggatgttg cgcaggtagt cgacctggcg cggcgtgcgg ccgtggagct ccgtacggcg
     2101 cgtcatcagc accggcgcgg gctgcgccgc ctcgcccttg ttggcgatct cgatgaggcc
     2161 gagctggacc tcgtcgcgcg acaggtcctt gtcggcgagc gcgtaaaaat gcttgagcgc
     2221 catctcgccg cgcagcacct gcgcggggtg gccctgcagc gtgaagtgtt cggcacgccg
     2281 gctcacggtg atgtcgaagg cgttctcgat ctggcgcagg ttctcgtcga gcacgccgca
     2341 cagcttggcc agccgggcgt tatccaccgg ctcgaagaag acttccagga ttttcgccat
     2401 tcaggattcc cgggtgagga tttcgccgcg caggctgtgc ggcagggctg cggtgatgcg
     2461 cacctccacg aactggccga cgaggcggtc gcggacgggc gagtcggagg ggaagttgac
     2521 cacgcggttg ttgtcgctgc ggcccatcat ctcggcctcg gcgttcttct tcgccgcgcc
     2581 ctcgaccagg atgcgctgca ccgtgccgac catggcctcg ctgttggcct ggtactgcgc
     2641 gtcgatgcgc ttttgcaggc gggcgagcca ggcgagcttg gtctcctgcg ccaccgggtc
     2701 ttcgaggtcg gcggccgggg tgccgggacg cgagctgtag acgaagctga aggagccgtc
     2761 gaagccgacg tcgtcgatca gcttcatggt cttctcgaag tcctcctcgg tctcgccggg
     2821 gaagccgacg atgaagtccg aggtcaagga aagatccggc cgcgccgcac gcagcttgcg
     2881 caccacggac ttgaactcga gcaccgagta gccgcgcttc atcgccgcca gcacgcggtc
     2941 cgagcccgac tgcaccggta gatggagctg agagacgagc ttggggatct tcgcgtagca
     3001 gtcgatcagg cgctgcgtca tctcgcgcgg gtgcgaggtg gtgtagcgga tgcgctcgat
     3061 gccggggatc tcggccacgc attccagcag gaaggcgaaa tcaccaacgt cggcgccctg
     3121 ctcgccgtcc tcgcgcgaga gcacgccacg ccaggcgttg acgttctggc cgagcagcgt
     3181 cacctccttg acgccctgtg ccgcgagacc ggcgatctcg gcgagcacgt cctcgagcgg
     3241 gcgcgacacc tcgtcaccgc gggtataggg cacgacgcag aaggtgcagt acttggagca
     3301 gccttccatg atcgacacga aggcgctcgc gccctcgacg cgcgccggcg gcatggcgtc
     3361 gaacttctcg atctcgggga aggagatatc cacctgcgag cgcccgctct gcttgcgcgc
     3421 cgcgatcagg ctcggcaggc ggtgcagggt ctgcgggccg aacaccacgt ccacataggg
     3481 cgcgcgcttg acgatggcct cgccttcctg gctggccacg cagccgccca caccgatgat
     3541 cagcccgggc ttcttctgct tgagcagacg cacgcggccg agatcgtgga acacccgctc
     3601 ctgcgccttc tcgcgcaccg aacaggtgtt gaagaggatc acgtcggcct cttccgggtt
     3661 gtcggtcttg accagttctt cgtgagcgcc gagcacgtcc gccatcttgt cggagtcgta
     3721 ctcgttcatc tggcacccga aggtgcggat gtagagtttc ttcatctatc agggaatgtc
     3781 aggcaaggcc ttgcggcagg tcgggcgggc atcgcggcat ccatcaaggc cgggacggcg
     3841 ccttgggcat cggcgccgca gcctcttccg gggtgaggat ccacacccga tgcacctggc
     3901 cgttgcggtc gaccagcatg cgcaccacgt actcgccaga cacatgcgag ggcagcacga
     3961 tgcggttgtg ggtatcgcgg atctgcgcgc cggggctgag ttgcagcgtg gcatccttga
     4021 cctgcaccac gctggccgag gaaaacgtca tcttcacccg cgccgcgtcg tcgggaatcg
     4081 gccgcggcaa ggcagcaaag gccacggacg acgcgaggcc ggagagaccg gccagcagga
     4141 cggagaggaa atgcgggcgc aaggacgggc cggaaagatc cggaacggta gggaacaggg
     4201 cgccgatgat cgtccatcgc cccggggatg tcaatgcaaa agccttataa atcaagcgtt
     4261 ccactggcag aaccctagcc cctgcacgca cttgcggagc ctactgcgcg cgcatggccg
     4321 ccctgccccg gtgccgcggc gcacgcgcaa ccggcatggc cccatccgca gaaggtcctc
     4381 ttcgggcgcg ctgacgcttg acgaccccgc cgcagacccg ctatattcgc gcgctctgtt
     4441 ggtgatgtag ctcagacggt tagagcgatg gattcataac ccataggtcg gcggttcgat
     4501 tccgcccatc accaccacca gatacgaagc cgctgatccg cgagggtcag cggctttttt
     4561 catctctgaa gcctctgcgg gtggttcgca gagccgggtc gctcgggact cgcgtagcaa
     4621 aacgatcgct ccgtctcgcc gcccgcttgc agtccgcaag caaaccgcgt cttcctccac
     4681 ggacgaacgc cccgtaacga ggcatgattg ctccatgtcc tccacccatg tcgccaccct
     4741 tcccaagcag cctccccgcc gcccgcccca tcgcgacggc gggcggcgct tcctgctgct
     4801 ggcgggggga tactggaact gcgcgcgcaa gtggcaggtg cgcgggacgg tgctggcgct
     4861 ggcgctgctg acgatggcgc aggtggggct ggcgatctgg gtgagctact ggaaccgcga
     4921 cctcttcgac gccctggagg agcgcgcgct gtccgagctg ctgcagctgg tcggggtctt
     4981 cgtgctgatc ttcgccctca ccatgggggt gacggcgctg cacatgcacg tcaagcgccg
     5041 gctgcagctc gactggcggc gctggctcac cgacctgctg ctcgagcact ggctgtcgca
     5101 cggccacctc taccgcctgc aattcaccgc cggcgagcac gacaaccccg acggccgcat
     5161 cgccgaggac atccgcatcg ccaccgacgt ggcggtctcg ctcgcccact cgctgctgtt
     5221 cgcgctgctg atcctgggca gcttcatcga catcctgctg cgcgtctcgg gcagcgcgcc
     5281 gctgccgggg acctcgatcg cggtgcacgg ctacatggtg ctcgccgcct tcctgtatgc
     5341 gggcgtgggc gcggcgctcg ggctcatgct cggtcgcccg ctgatccgcg ccaccaaccg
     5401 cctgcagacc gtcgaggcca acctgcgctt cgggctggcg cgcgcgcgcg agcacgccga
     5461 gccgatcgcg atgatgcacg gcgagccgca cgagcgccgc gaggcggcgc gcctgttcga
     5521 cgacgtcggg cggggctgga accggcagac cctggcctac ctcggcatcg tgtccttctc
     5581 caccggctac ggcaccctgc tgccggtgtt cccgatcctg atcgcggcgc cgcaatacat
     5641 cgccggcgcg atgacgctcg gcgtgctgat gcaggcggcg caggccttcc agcgcctgac
     5701 cacggcgctg tcgtggccga tcgacaacct cggcgagctg gcgcgctgcc gcgcctcgat
     5761 cgaccgcgtc gccagcctct acgaggacat gctcgcgctc gagcgcgccg aagcccgcag
     5821 ccgcggccgc gagcgcatcg cggtgtgcag ctcgccccac cccgagctcg agctgcgtcg
     5881 gctgcgcctg cagacaccgt cgggcgagat cctcctcgac gacttcgacc tgcgcgtgcg
     5941 ccgcggcgag cgcgtgctga tctgcggcga ccctgcggtc accgtgagcc tgttcaaggc
     6001 ggtggccggg ctgtggccgt ggggcagcgg ggaggtgagg ctgccgctcg gacgcgacct
     6061 gatgttcgtg ccgcgccagc ccttcctgcc cgcgggcagc ctgcgcatgg tgctgagcta
     6121 tcccgacgac gccgaccgcc acgccgacgc cgtgctcgag cgtgcgctcg aatgcgccgg
     6181 catcgcctgg ctggccccca agctcgacac ccaggaggac tgggggcgcg agctgccgct
     6241 gcgcatccag cagcgcctgg gcgtcgcacg cgtgttcctg caccagccgg ggtggatctt
     6301 catcgaggag gccaccgacg cgctcgcgcc caaggaagag gtcgacaccc tggagctgct
     6361 gcaccacgag ctgcccagct cgaccctgct ggtgatcagc cggcgcgcac gccagaacca
     6421 cttcttcgag cgccggctac tgctgcagcg cccgcgcccg ggcctgcagc cgccggccgc
     6481 acccgcggcg cggccggaaa cgccctccct acccgaagcg tgagggcagc cagccgcgct
     6541 ccacgcacgc gcgcaagcac caggccggcg tggtctcggt cttgtagctc cagaagaacc
     6601 agcccgccag gcgttcgaag gtcatcagct ggctgtcgcc gtaggcgcgg ctggccacgt
     6661 cctgctggaa gtcgtccatg tgctcgaggg cgtggttgaa cggcccctcg gcccacagcg
     6721 acaccacctt caggtccagc cccaggctcc actcgccgca caccgccggc aggccgagct
     6781 cggcgttgat cgcgtcggct tcctcgcgcc agtccaccgc cgccttgcgg atgtggccgt
     6841 ggatgtccat gtcgatgtcg ccgcgctcga agcactggta gcggtgatag tcgaacacca
     6901 cgttgcggaa ggccggcgcc tgcatgaagc cgaggtactc gcggaagctg cggaagccgt
     6961 cgtggaacac caccgccacc gtctccggcg cgcagtgctt gcggatgcgc gcgtaggcct
     7021 cgaggtagta ggacttcagg tagtcggtcg gcacgtccca gcgcggctcg ttgagcagct
     7081 cgatgccgtg cagcgccggg tgcgcgcggt agcgctcggc caggcgctcg agcacggcga
     7141 gcgagtgctc gaggtattcc ttcttcgtat gccactccac cacgtccttg atgccgccgt
     7201 tatcgaagcc gttctggcag ccgggcgcgg cgtgcaggtc gatgatcacg cgcaggccga
     7261 actcctgcgc ccagtcgagg gcgcggtcga gcacctcgat gccgccggtg acgaaggggt
     7321 ggcggtgggc gccgtacttg gggtggtagg ggtagtcggg gccgaagatc cagtgcccga
     7381 tcgggatgcg caccgcgttg aggccgcgtg cggcgatcca ggcgaagtcc tcgcgggtca
     7441 cgaagctcgc ccagtgccgg cgcaggcgct cggccgcggc gggaccgagc tcggcgcacc
     7501 aggtggtctc gtcggtcgcc tccaggccct cgaacaggct gggcaccatc cacttctcca
     7561 gcagcagcca gctacccagg ttcaccccgc gcagcttggt acgcgtcgtt tccatgagaa
     7621 cctccccttg cccggatcgg gcgtgatgga catggctcat tctggcagcc aaacgcctcg
     7681 cccgaccgtg aaaaagctcg cgcccactgt gcgccgccct ttcgcgactt gcgtcgctcc
     7741 cacgggccgg gccacgccgc ggcggcaatg tgattctcgt aggagcgacg caagtcgcga
     7801 tcgtgactcg acacggtgac acacgtggca gaatcgcccc ccgccccgcc cgagtccgcc
     7861 ccatgcgcac cgtcgaacac gtcgtcaccc acctgatcct ccacgccctg gtacgcgagc
     7921 ccggcgcgcc ctcgcgcctg atgctgcgcg agaagccgtg cgcgctcgac gccgccgcgc
     7981 tgcgcctggt cgagcgcctg tgcgcgcagt acgagagccg cagcgccaag ggcttcggtc
     8041 gcctcgagga ggacgaggag agctacccgc tcgcgcgctg gctgcgcgag cacctcgtcg
     8101 acgagaccct cgacttcacc gccctctccc gccagctcgc cgagcgcgtg caggcgatcg
     8161 ccgacgagga agagctggaa gaaggcggcc atctcgtgct cgcgcgcatc cgcgagggcg
     8221 agggcgacag cctgtgggtg gcgctgctcg gcagcagcga cggggtggcg atcggcggcg
     8281 acctcgcacc gctcgactgc acccatgtgg acttctccgc gctgcacgcc gccgggcggg
     8341 tcgacctcgc cggctggcgg cgcggcgacg agcgctacct cggcttcctg cgcggacgcg
     8401 gcaagggcgg cgcctggttc aagcgcgtgc tcggctgcag cgacgtgatg gtggcgctgc
     8461 aggagaccaa gaagctggtc gccacgctcg accgcttcgt cgacaccgag cgcctcgccc
     8521 ccgccacccg cgacgccgtg ctcgagcgcg cccacgacta cctcgacacc ctcggcgagg
     8581 ccggcgcccc ggtggtgttc gaggagctcg cgcgcgaggt cttccccga
//
LOCUS       DAOQNN010000104         8604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4087_length_8604_cov_9.479237, whole genome shotgun sequence.
ACCESSION   DAOQNN010000104 DAOQNN010000000
VERSION     DAOQNN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4087_length_8604_cov_9.479237"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="PKV97_07515"
     CDS             complement(<1..1486)
                     /locus_tag="PKV97_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNB05761.1"
                     /translation="MTAELETILERHRRDPLQLLQILIELQARDGWLPPATLSALAGA
                     LGIPRARVESTASFYSFLHTRPAGEYRILFSDNITDRMLGNQALMQTLCDKLWLQPGK
                     VSEDGLVSVATTSCTGMCDQGPALLANGRTITRLTLERIDEMAHLIRRRVPVGDWPPE
                     WFHVEDKLRRRDVLLDHGLAPGAALAAALERGPTGLLWEIERSGLRGRGGAGFGSDIK
                     WRSCRDAWGDAHYVICNADEGEPGTFKDRVLLSSYFDLVVDGMCIAGMAIGANKGFIY
                     LRGEYRYLLDRLETRLAQRREQNLLGRNILGRGFSFDIEIHLGAGAYICGEESALIES
                     LEGKPGKPRIRPPFPVTNGYRGQPTTVNNVETLALAALIAVRGGDWFHAIGTPASTGT
                     KLLSVSGDVERPGIYEFPFGVTVAEVLEAAGARDTQAVTSAGAAGHCLAADEFGRRIA
                     FEDVATGGSIMVFDRSRDMFEVAHNFAHFFAHESCGFCTPCRVGT"
     gene            complement(1641..4115)
                     /locus_tag="PKV97_07520"
     CDS             complement(1641..4115)
                     /locus_tag="PKV97_07520"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNB05762.1"
                     /translation="MDLAQFTLQYDRPSTDVESLKRAIANKLMYQIGKNPESALPEDW
                     LHAVAYTVRDHLVERWMKTTRASYAQDVKRVYYLSMEFLIGRSLGNAMLALELAGPVR
                     EALRLLDIDPDSLPELEPDAALGNGGLGRLAACFLDSMATLGVPGFGYGIRYDYGMFR
                     QQIVDGQQVEVPDYWLTHGNPWEFPRPEIRMRIRFGGHLQEDGGRVHWVGTDDVLAMA
                     YDSIIPGYGTEVTNTLRLWSARATEEIDLSAFNRGNYFGAVESKNHSENVSRVLYPDD
                     STLEGRELRLRQEYFFVSASLQDILRRYLVGHTDFDALPDKVSIHLNDTHPVLAVPEL
                     MRLLVDERGLEWARAWALCRRVFSYTNHTLMHEALETWPLEMLGRVLPRHLRIIFDIN
                     AHFLAELNAELGPDVERMRRMSLIDEQGERRVRMAYLAIVASHSVNGVSALHSALMRE
                     SIFADFARAWPQRFNNKTNGVTPRRWLAHANPGLAALIDSRIGPAWRRDLEQLQGLRA
                     HADDADFLHTLRAVKQANKLRLAQWIGQHCGLHVDPAALFDVHVKRIHEYKRQLLNAL
                     HLVSRYQRILAEPNGEHVPRVAIFSGKAASAYRMAKLVIRLINDVAAVINADARVGER
                     LKVVFVPNFSVSLAELIIPAADLSEQISTAGTEASGTGNMKLALNGALTIGTLDGANV
                     EIREQVGEDNIFIFGHTAAEVAQIRNGGYQPRQYYERDARLAAALDAIRAGLFSPDEP
                     GRYQAIFDTLVNWGDHYLLLADYASYLDAQDQADARYRDPLQWSRCSLLNIAGMGAFS
                     SDRTIAEYARTIWHTEPLRIGSPGGL"
     assembly_gap    4245..4344
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4429..5013
                     /locus_tag="PKV97_07525"
     CDS             4429..5013
                     /locus_tag="PKV97_07525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB05763.1"
                     /translation="MSSDPFPEPRRHPSLHGAGGAPSDWVRRFTALLADGARVLDFAA
                     GGGRHARWLAARGCSVTAVDRDAAALATLDGVAGIATRVLELEGADWPLGGERFDAVV
                     VANYLYRPRLAELFELVRPGGVLIYETFMLGNERFGKPSNPDFLLRPRELLDCLPEGW
                     SVVAFEQGEIERPAMIQRLCATRGASPGRLPQSV"
     gene            complement(5227..6279)
                     /gene="mnmH"
                     /locus_tag="PKV97_07530"
     CDS             complement(5227..6279)
                     /gene="mnmH"
                     /locus_tag="PKV97_07530"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNB05764.1"
                     /translation="MQKGVATVAQLSGFDEIIDARTPAEFAEDHIPGAINLPVLDNDE
                     RIVVGTMYKQQSAFDARRLGGALVAANISRHMLAVLQDKPKNWRPLIYCWRGGQRSGS
                     FTTWLRMVGWDACQLEGGYKAFRHLVIAEIERIVPTLALRIVCGPTGSAKTRVLEALA
                     AQGAQVLDLEDLAAHKGSVLGALPDRPQPAQKRFETLLWDRLRRLDPARPVYVEAESR
                     KIGLVFVPEALILAMRRAPCILIDATRAARLEFLVRDYAYLGDDVGALQDKLHRLREH
                     QSRETLERWKSLAAAHALPALFAELIDQHYDPLYHRSQNHNYVHYADALRVESADLSA
                     AGVRALAGRILAEAAE"
     gene            6339..7379
                     /gene="selD"
                     /locus_tag="PKV97_07535"
     CDS             6339..7379
                     /gene="selD"
                     /locus_tag="PKV97_07535"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNB05765.1"
                     /translation="MNEIRLTEFSHGGGCGCKIAPAVLAELLATMPAGLVPPELLVGT
                     DTADDAAVYKLNDRQAIVATTDFFTPIVDNPRDFGRIAATNALSDVYAMGAQPILALA
                     IVGMPLDKLPPAVIGEILKGGAEVCRDAGIPVAGGHSIDVLEPIYGLVGLGVVDPARV
                     RTNAGAKAGDVLILTKPLGIGILSAGLKKGRLSADGYAQMIRWTTTLNRVGARLADLD
                     GVHAVTDVTGFGLAGHLLEMCRGASLTGAVRFDALPVIEEARALVRDGVATGASTRNW
                     ASYGASVELPVDAPEWQRKLVTDPQTSGGLLIACAPEAVEAVQAAVRAEQGEAGTIVG
                     EMKAGAARVVVA"
     gene            complement(7642..8277)
                     /locus_tag="PKV97_07540"
     CDS             complement(7642..8277)
                     /locus_tag="PKV97_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNB05766.1"
                     /translation="MPLRFIIPFAFMFLFGAVARADTILVWGDSLSAGYGLERGQEWP
                     TLLQTRLQAEGFRHVVVNASVSGETSSGGLTRLPAALAEHAPTLVILELGANDGLRGL
                     KPQLLADNLAAMIDAAHAVGARVLLIGMRMPPNYGPAYTQRFAEVFANVAAEKKVPLV
                     PFLMDGFADRPELFLPDGIHPAAEAQPRIVDTVWAGLRPLLAAKETKAARK"
     gene            8261..>8604
                     /locus_tag="PKV97_07545"
     CDS             8261..>8604
                     /locus_tag="PKV97_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB05767.1"
                     /translation="MKRNGIELSNPVIEVDDLCKQVALADGQGELRILQDIRFAVGAG
                     ESVAIVGASGSGKSTLLGLLAGLDVPAHGTVRVQGRDLFALDEDARAALRGEAIGFVF
                     QSFQLLPALTAL"
BASE COUNT         1203 a         2855 c         3111 g         1335 t
ORIGIN      
        1 cggtgccgac gcggcagggc gtgcagaagc cgcagctctc gtgggcgaag aagtgggcga
       61 agttgtgcgc cacctcgaac atgtcgcggg aacggtcgaa caccatgatc gagccgccgg
      121 tggcgacgtc ctcgaaggcg atgcgacgac cgaactcgtc ggccgccagg caatgcccgg
      181 ccgcgccggc gctcgtcacc gcctgcgtgt cgcgcgcgcc ggcggcctcg agcacctcgg
      241 ccacggtgac accgaaggga aactcgtaga tgcccgggcg ctcaacgtcg cccgacaccg
      301 acagcagctt ggtgccggtc gaggccgggg tgccgatggc gtggaaccag tcgccgccgc
      361 gcaccgcgat cagcgcggcg agggcgagcg tctcgacgtt gttcaccgtg gtcggctgcc
      421 cgcgatagcc gttggtgacc gggaagggcg ggcggatgcg cggcttgccc ggcttgcctt
      481 cgagcgactc gatcagcgcg gattcctcgc cgcagatgta ggcgccggcg ccgaggtgga
      541 tctcgatgtc gaaggagaag ccgcggccca ggatgttgcg cccgagcagg ttctgttcgc
      601 ggcgctgggc gaggcgtgtt tccaggcggt cgagcaggta gcggtattcg ccgcgcaggt
      661 agatgaagcc cttgttggcg ccgatcgcca tgccggcgat gcacatgccg tcgaccacca
      721 ggtcgaagta gctcgacagc agcacgcggt ccttgaaggt gccgggctcg ccctcgtcgg
      781 cgttgcagat cacgtagtgc gcgtcgcccc aggcgtcgcg gcacgagcgc cacttgatgt
      841 cgctgccgaa acccgcgccg ccgcggccgc gcagtccgga gcgctcgatc tcccacagca
      901 gcccggtggg gccgcgctcg agcgccgcgg cgagcgccgc acccggcgcc aggccatggt
      961 cgagcagcac gtcgcggcgg cggagcttgt cctcgacatg gaaccactcc ggcggccagt
     1021 cgcccaccgg cacgcggcgg cggatcaggt gcgccatctc gtcgatgcgc tcgagcgtga
     1081 ggcgggtgat cgtgcgcccg ttggccagca gcgctggccc ctgatcgcac atgccggtgc
     1141 atgacgtagt ggccacgctc accaggccat cctccgacac cttgcccggc tgcagccaga
     1201 gcttgtcgca cagcgtctgc atcagcgcct ggttgccgag catgcggtcg gtgatgttgt
     1261 cggagaacag gatgcggtat tcgcccgccg gccgggtgtg caggaagctg tagaagctcg
     1321 cggtgctctc gacgcgggcg cgcgggatgc cgagcgcgcc cgccagcgcg gagagcgtgg
     1381 ccggcggcag ccagccgtca cgggcctgga gctcgatcag gatctgcagc aactgcagcg
     1441 gatcgcgccg gtgacgctcc aggatggttt cgagttctgc cgtcatcgcc gcaagctccc
     1501 tgtgattgcg tgcccctgcc gcgcgattat cgcccgccgc actcctcgac gctcatggaa
     1561 agcgtgcgcg gggccgatgc attgcactta ttcgtgccga tcgcgccgcg ggcggctccc
     1621 atgccgccgc ccgcgtgcct tcatagcccg cccgggctgc cgatgcgcag cggctcggtg
     1681 tgccagatcg tgcgcgcgta ctcggcgatg gtgcggtcgg acgagaacgc ccccatgccg
     1741 gcgatgttga gcaggctgca gcggctccat tgcagcggat cgcggtagcg cgcgtcggcc
     1801 tggtcctggg cgtcgagata gctggcgtag tcggccagca gcaggtagtg gtcgccccag
     1861 ttcaccaggg tgtcgaagat cgcctggtat cgccccggct cgtccgggct gaagaggccc
     1921 gcccggatcg cgtcgagcgc cgcggccagg cgcgcgtcgc gctcgtagta ctggcgcggc
     1981 tggtagccgc cgttgcggat ctgcgcgacc tcggctgcgg tgtggccgaa gatgaagatg
     2041 ttgtcctcgc cgacctgctc gcggatctcg acgttggcgc cgtccagcgt gccgatggtg
     2101 agcgcgccgt tgagtgcgag cttcatgttg ccggtgcccg aggcctcggt gccggcggtg
     2161 gagatctgct cggagaggtc tgcggccgga atgatgagct cggccaggct gacgctgaag
     2221 ttgggcacga agaccacctt gaggcgctcg ccgacgcgcg catcggcgtt gatgaccgcg
     2281 gccacgtcgt tgatgaggcg gatcaccagc ttggccatgc gataggccga ggccgccttg
     2341 ccggagaaga tcgccacccg cggcacgtgc tcgccgttcg gctcggcgag gatgcgttgg
     2401 tagcggctca ccaggtgcag cgcgttgagc agctggcgct tgtactcgtg gatgcgcttg
     2461 acgtgcacat cgaagagcgc ggccggatcg acgtggagcc cgcagtgctg gccgatccac
     2521 tgcgccagac gcagcttgtt ggcctgcttg accgcgcgca gcgtgtgcag gaagtcggcg
     2581 tcgtcggcgt gcgcgcgcag gccctggagc tgctcgaggt cgcgccgcca ggctggaccg
     2641 atgcggctgt cgatcagcgc cgccagccct gggttggcgt gcgccagcca gcgccgcggc
     2701 gtcacgccgt tggtcttgtt gttgaagcgc tgcggccagg cgcgcgcgaa gtcggcgaag
     2761 atcgactcgc gcatcagcgc cgaatgcagc gccgaaacgc cattgaccga gtgcgaggcg
     2821 acgatcgcga ggtaggccat gcgcacgcgg cgctcgccct gttcatcgat cagcgacatg
     2881 cggcgcatgc gctcgacgtc gggcccgagc tcggcgttga gctcggcgag gaagtgcgcg
     2941 ttgatgtcga agatgatgcg caggtggcgc ggcagcacgc ggccgagcat ctccagcggc
     3001 caggtctcga gcgcctcgtg catcagggtg tggttggtgt aggagaacac ccgccggcac
     3061 aacgcccagg cgcgcgccca ttccagcccg cgctcgtcga ccagcaggcg catcagctcg
     3121 ggcaccgcga gcaccgggtg ggtgtcgttg aggtggatgc tgaccttgtc gggcagggcg
     3181 tcgaagtcgg tgtggccgac aaggtagcgg cgcaggatgt cctgcaggct ggcggagacg
     3241 aagaaatact cctggcgcag gcgcagctcg cggccctcga gggtggagtc gtccgggtag
     3301 agcacgcgtg agacgttctc ggagtggttc ttgctctcaa ccgcgccaaa atagttgccg
     3361 cggttgaagg ccgacaggtc gatctcctcg gtggcgcgtg ccgaccacag gcgcagggtg
     3421 ttggtcacct cggtgccata gccggggatg atcgagtcgt aggccatcgc cagcacgtcg
     3481 tcggtgccca cccaatgcac gcgcccgcca tcctcctgca ggtggccgcc gaagcggatg
     3541 cgcatgcgga tctcggggcg ggggaactcc cacgggttgc cgtgcgtgag ccagtagtcg
     3601 ggcacctcga cctgttgccc gtcgacgatc tgctggcgga acatgccgta gtcgtagcgg
     3661 atgccgtagc cgaagccggg cacgccgagc gtggccatgg agtcgagaaa gcaggcggcg
     3721 aggcgaccga ggccgccgtt gccgagcgcg gcgtcgggct cgagctcggg caggctgtcg
     3781 gggtcgatgt cgagcaagcg cagcgcctcg cgcaccgggc cggcgagttc gagggcgagc
     3841 atggcgttgc ccaggctgcg gccgatgagg aactccatcg acaggtaata gacgcgcttg
     3901 acgtcctgcg cgtagctcgc ccgtgtggtc ttcatccagc gctcgaccag gtggtcgcgc
     3961 acggtgtagg caactgcgtg cagccagtct tccggcagcg ccgactcggg gttcttgccg
     4021 atctggtaca tcagcttgtt ggcgatcgcg cgtttgagcg actcgacgtc ggtgctgggg
     4081 cggtcgtact ggagggtgaa ctgggcaaga tccatggtga cgtcggtgaa ggtccggtaa
     4141 tcgggtggcg cggccgcggc cggcgtggtg acgtggctaa atccgctagt ttaggccttt
     4201 cgcctgcaat ggcgctcgat cgcccgcccg tgcctgtgcg tcggnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnggcatg ccgtgcgtcg ctagaatgcg gtggccacgc
     4381 ccggtcttgc cgcgcccccg cgacgaggct tgcatccaag gagcccccat gtcttccgat
     4441 cccttccccg aaccccgccg tcaccccagc ctgcacggtg ccggcggcgc tccctccgat
     4501 tgggtacggc gcttcaccgc gctgctcgcg gacggcgcgc gcgtgctcga cttcgccgcg
     4561 gggggcggcc ggcatgcgcg ctggcttgcg gcgcgcggct gcagcgtcac cgcggtcgat
     4621 cgggacgccg cggcgctggc gacgctggat ggggtggcgg ggatcgcgac gcgagtactc
     4681 gagctcgagg gtgcggactg gccgctcggc ggcgagcgct tcgacgccgt ggtggtcgcc
     4741 aattacctgt accggccgcg cctggcggag cttttcgagc tggtgcgccc gggcggcgtg
     4801 ctgatctacg agaccttcat gctcggcaac gagcgcttcg gcaagccctc gaaccccgat
     4861 ttcctgctcc gtccgcgcga actgctcgac tgccttccgg agggctggag cgtggtggcc
     4921 ttcgagcagg gcgagatcga gcgtccggcg atgatccagc gcctgtgcgc gacccgcggc
     4981 gcatcgccgg gccgcctgcc acaatccgtc taggcttggc ggccacctgg gcgaagatgc
     5041 gacggtgtcc gcccctcggc cggagagatc gcgacctgcg tcgccgcaac gggccgaccc
     5101 gatcgcgact cgcgtcgccc ccaccggccg atgcggacgt cggcgccaca gggaacgcgc
     5161 cggttctttg aggaaacgcg cgtcccgatt ggggtggcgg ggctgctcgg gctcgccccg
     5221 gacgcttcac tccgcagctt cggcgagaat gcgaccggcc agcgcgcgca ccccggccgc
     5281 gctcaggtcg gcgctctcca cccgcagcgc gtcggcgtag tgcacgtagt tgtgattctg
     5341 cgaacgatgg taaagcgggt cgtagtgctg gtcgatcagc tcggcgaaca gcgcgggcag
     5401 cgcgtgtgcg gcggcgagcg atttccagcg ctcgagcgtc tcgcggctct ggtgctcgcg
     5461 caggcggtgc agcttgtcct gcagcgcgcc cacgtcgtcg ccgaggtagg cgtagtcgcg
     5521 caccagaaac tcgaggcgcg ccgcgcgcgt ggcgtcgatc aggatgcagg gcgcgcgccg
     5581 catcgccagg atcagcgcct cgggcacgaa gaccaggccg atcttgcggc tctcggcctc
     5641 gacatacacc gggcgcgccg gatcgagccg gcgcaggcga tcccacagca gggtctcgaa
     5701 gcgcttctgc gccggctgcg gacggtcggg cagggcgccg agcaccgagc ccttgtgcgc
     5761 ggcgaggtcc tccaggtcga gcacctgcgc cccttgtgcc gcgagcgcct cgagcacgcg
     5821 cgtcttcgcg ctgccggtcg ggccgcacac gatgcgcaac gccagcgtgg gcacgatgcg
     5881 ctcgatctcg gcgatcacca ggtggcggaa ggccttgtag ccgccctcga gctggcaggc
     5941 gtcccagccc accatgcgca gccaggtggt gaaggagccg ctgcgctggc cgccgcgcca
     6001 gcagtagatg agggggcgcc agttcttggg cttgtcctgc agcaccgcga gcatgtggcg
     6061 cgagatgttg gcggccacca gcgcgccgcc gaggcggcgg gcgtcgaagg ccgactgctg
     6121 cttgtacatc gtgccgacga cgatgcgctc gtcgttgtcg agcaccggca ggttgatcgc
     6181 gccgggaatg tggtcttcgg cgaactcggc gggcgtgcgc gcgtcgatga tctcgtcaaa
     6241 gccggacagc tgtgctacgg tcgcgacgcc tttttgcatg atgttgacac gacgcgcggc
     6301 gcccacttgc gccgcgcgtc gctttctctc ccggaaacat gaacgaaatc agactgaccg
     6361 aattctccca cggcggcggc tgcggctgca aaatcgcccc cgccgtgctc gccgagctgc
     6421 tcgccaccat gcccgccggc ctcgtgccac ccgagctgct ggtggggacc gacactgccg
     6481 acgacgccgc cgtctacaaa ctcaacgatc gccaggccat cgtcgccacc accgacttct
     6541 tcaccccgat cgtggacaac ccgcgcgact tcggccgcat cgccgccacc aacgcgctct
     6601 ccgacgtcta tgcgatgggc gcccagccca tcctggcgct ggcgatcgtc ggcatgccgc
     6661 tcgacaagct gccgcccgcg gtgatcggcg agatcctcaa gggcggcgcc gaggtctgcc
     6721 gcgacgcggg catcccggtc gccggcgggc actcgatcga cgtgctcgag ccgatctacg
     6781 gtctggtcgg cctcggcgtg gtcgaccccg cgcgcgtgcg caccaacgcc ggcgccaagg
     6841 cgggcgacgt gctcatcctc accaagccgc tcggcatcgg catcctctcg gcgggcttga
     6901 agaagggccg gctgtcggca gacggttacg cgcagatgat ccgctggacg acgacgctca
     6961 accgtgtcgg cgccaggctc gccgacctcg acggcgtgca cgcggtgacc gacgtcaccg
     7021 gcttcggcct cgcggggcac ctgctggaga tgtgccgcgg cgcgtcgctg accggcgcgg
     7081 tgcgcttcga tgcgctgccg gtgatcgagg aggcgcgcgc cctggtccgg gacggcgtcg
     7141 ccaccggcgc gtcgacgcgc aactgggcga gctacggtgc cagcgtggag ctgccggtgg
     7201 acgccccgga gtggcagcgc aagctcgtca ccgacccgca gacctccggc ggcctgctga
     7261 tcgcctgcgc gccggaagcc gtcgaggccg tgcaggccgc ggtgcgcgcc gagcagggcg
     7321 aggcggggac gatcgtcggc gagatgaagg cgggcgcagc gcgggtggtg gtggcgtaag
     7381 cgccacgtgc gtttccgggg aggggcggtc gtccggcatc gggcgccgtg ctcccctgcc
     7441 ttcggccgcg acgcgcgacc gggcagtgcg atcgcgactg gcgtcgctcc tacagccagc
     7501 cctcggtgtt tcggttcttc gtaggagcga cgcgagtcgc gatcgggcga tcgtgccggg
     7561 acgggcgtcg gtcgggtccc gtgcgccacg atcgcgcctc gcagcgctcc tgcggctgaa
     7621 ttccccccgg cgcttcgagc ctcacttgcg cgccgccttc gtctccttcg cggcaagcag
     7681 cgggcgaagg ccggcccata ccgtgtcgac gatgcgcggc tgcgcctcgg cggccgggtg
     7741 gatgccgtcg ggcaggaaga gctcggggcg gtcggcgaag ccgtccatca ggaagggcac
     7801 gagcggcacc ttcttctccg cggccacgtt ggcgaagacc tctgcgaagc gttgcgtgta
     7861 ggccggacca tagtttggcg gcatgcgcat gccgatcagc aggacgcgcg cgcccaccgc
     7921 gtgcgcagcg tcgatcatcg cggccaggtt gtccgcgagc agctgcggct tgagtccgcg
     7981 caggccgtcg ttggcgccga gctccaggat caccagcgtg ggcgcatgtt cggccagggc
     8041 ggcgggcagg cgggtgaggc cacccgacga ggtctcgccg gatacactgg cgttgaccac
     8101 gacatggcgg aacccttcgg cctgcagacg cgtctgcaac agggtgggcc attcctggcc
     8161 cctttccaga ccgtagccgg ccgacaggct gtcgccccat accaggatgg tgtcggcgcg
     8221 ggcgacggcg ccgaaaagga acatgaacgc gaagggaatg atgaaacgca acggcatcga
     8281 gctctccaat ccggtcatcg aggtcgatga cttgtgcaag caggtcgcgc tggccgacgg
     8341 ccagggcgag ctgcggattt tgcaggacat ccgctttgcc gtgggtgcgg gcgagtcggt
     8401 ggcgattgtg ggcgcctccg gctccggcaa atcgaccctg ctgggattgc tcgccgggtt
     8461 ggacgttccc gcgcacggca cggtgcgggt gcagggccgc gacctcttcg cgctcgacga
     8521 ggacgcgcgc gcggcgctgc gcggcgaggc gatcggcttc gtgttccagt ccttccagct
     8581 gctgccggcg ctcaccgcgc tgga
//
LOCUS       DAOQNN010000105         8583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4113_length_8583_cov_9.594629, whole genome shotgun sequence.
ACCESSION   DAOQNN010000105 DAOQNN010000000
VERSION     DAOQNN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8583
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4113_length_8583_cov_9.594629"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..270
                     /gene="ftsL"
                     /locus_tag="PKV97_07550"
     CDS             4..270
                     /gene="ftsL"
                     /locus_tag="PKV97_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNB05768.1"
                     /translation="MVRIDAVLVALAVASALGVVASQHQARRLHGEYEREQARAQALE
                     VEWGQLQLEQSTWATHGRIEKLARDRLHMQLPPEGQVLVVEPQS"
     gene            267..2033
                     /locus_tag="PKV97_07555"
     CDS             267..2033
                     /locus_tag="PKV97_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNB05769.1"
                     /translation="MKKQRAVTFNHNPLLKRELPAWRPRIIMLALMGCSLALVGRALY
                     LQGVNNDFLQAKGESRYARVLEVPATRGRITDRHGDILAVSTPVRAVWALPADARLEP
                     AQVRELARLLEMNVGELNARLAAGRDFVYLKRQVPPEVAQAVADLRLPGIHQEKEYRR
                     YYPGGEVMAHILGFTDLEDRGQEGIELAFDKRLSGRAGARRVIKDRRGQIVEDVEAIR
                     PPRDGDEVALSIDGKIQYLAWSALREAMEEHKPKAAAAVVLDVRTGEVLALVNAPTFN
                     PNNRANLTGAQLRNRVFTDAFEPGSVMKPFIAALALERGKVKLNTPIDTGNGRMTIGT
                     ATISDTKRHGVITMAQVVQKSSNIGTVKMAQQLGAEEMWHLFDQLGFGTPLNLGFPGE
                     TSGRLRPAKTWRPIEQATMSYGHGISTSLIQMARAYLVFAREGELVPLSLTRVDGTPS
                     SGRRVFSADTARQMRTVLAMAAGPGGTAIRAQVPGYTVAGKTGTAHKLEDGRYARKYI
                     SSFVGFAPASDPRIVVAVMVDEPSGGQHYGGTVAAPVFSRITEGTLRTLGVAPDMPLT
                     PLQLARRGGDEAASEGSSRESM"
     gene            2039..3535
                     /locus_tag="PKV97_07560"
     CDS             2039..3535
                     /locus_tag="PKV97_07560"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNB05770.1"
                     /translation="MQALAVLDRLRALGVHADDITADSRKAGPGCVFAAWPGQRTDGR
                     RFLADAVVHGAAALLWEDGDGFCAPALAVPGFGVRGLRELAGHLAHELHGRPSQSLWL
                     AGVTGTNGKTTVSQMLARALGELGERCGIVGTLGCGFPDALEEGLNTTPDALELQRWF
                     ARFRNQGAVAAAMEVSSIGLDQGRVNGAQFDVAIFTNLSRDHLDYHGTMEAYAAAKAR
                     LFERPELSACVINIDDPWGLALARRLVVEGRAVIACTLYAANADAVPGARVLRADRLQ
                     AAPAGLRFTLVWEGREAEIGVRMVAHFNVSNLIAVAGALLARGIAFDELPPVLGRLAP
                     PQGRMQLVGGVCEPLVVIDYAHSPDALAKVLEALRGTVHSRRGRLVCVFGCGGDRDPG
                     KRPMMGEIASAIADRVVITSDNPRSEDPQRIVEAIAAGAGAAAECVVDRAQAITRAVG
                     EARADDVVLIAGKGHEPYQEILGQRLPFSDLEQAKRALAAWHARENGR"
     gene            3532..4944
                     /gene="murF"
                     /locus_tag="PKV97_07565"
     CDS             3532..4944
                     /gene="murF"
                     /locus_tag="PKV97_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNB05771.1"
                     /translation="MMSLHDATRALACHGARATGEGRFDSVGTDTRALAPGQLFVALR
                     GERFDGHDFVADAAKAGAAAAMVDAAWSAGKADAGLPLLVVDDTRRALGTLAAAWRAR
                     FALPLIGVTGSNGKTTVKEMCAAILRVQASRDGFGEESVLATRGNLNNDIGMPLTLLE
                     LRDFHRAAVIEMGMNHPGEIGYLSALAQPTVALVNNAQRAHLQGMGSVDEVAREKGAI
                     YEGLGGAGVAVINADDPHAGYWRSLNAGRPIVTFGIDHPADVSARCTLRGLGSRLELD
                     IPQGKIDFALQVPGLHNVRNALGAAAACLAAGVPLEAVAEGLAGFAGTRGRLQRRAGP
                     RGALILDDSYNANPDSVRVGIDVLAAMPGHTWLVLGDMGEVGETSAQVHDEVGGYAKS
                     KGIDGLFALGEMSAVAAHNFGEGARHFTSVEALVKALVPRLDADSVVLVKGSRFMRME
                     RVADALAAVHNPAPSTETGS"
     gene            4946..6034
                     /gene="mraY"
                     /locus_tag="PKV97_07570"
     CDS             4946..6034
                     /gene="mraY"
                     /locus_tag="PKV97_07570"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNB05772.1"
                     /translation="MLLELALWLGQDIRGFNVFGYITLRTVLAALTALAISLIAGPAV
                     IRWLAAKKIGQAVRDDGPKSHLTKAGTPTMGGALILIAIGVTILLWGDLKNAYVWVTL
                     LVTLGFGAVGWVDDWRKVVHRDPKGLASRWKYLWTSAIALAAAIYLGVTADTPAETEL
                     IVPFFKAIAYPLGIFGFIALTYFVINGTSHAVNLTDGLDGLAIMPTVMVAAALAIFAY
                     VAGHVGFSKYLGVPYVAGAGELAVFCGAICGAGLGFLWFNAYPAEVFMGDVGALALGA
                     ALGTIAVIVRQEIVLFIMGGLFVAETLSVMVQVLYFKATGGKRIFRMAPLHHHYELGG
                     WKETQVVVRFWIITIMLVLFGLSTLKLR"
     gene            6031..7431
                     /gene="murD"
                     /locus_tag="PKV97_07575"
     CDS             6031..7431
                     /gene="murD"
                     /locus_tag="PKV97_07575"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNB05773.1"
                     /translation="MSALTGKHVLVLGLGESGLAMARWCALRGARVRVADSRAVPPGV
                     DALKADAPLAEIFMGSFGDEVLDGVDLVALSPGLDPRVGVAAQARRRGLPLTGEMSLL
                     AQALDELGVRAQTRILAITGTNGKTTTTALTAALAQSVGIDAVAAGNISPAALDVLME
                     RLEIGAALPACWVLELSSFQIETMRGLDAEAATVLNVTDDHLDRYADLEEYAATKAAI
                     FQGEGVQVLAREDARVAAMALPGRRTIRFGTAAPASETDYGLADLDGRAWLVRGGERL
                     LALADLPLAGRHNAANALAALALCEGGLGIAPQALIPGLKAFRGLPHRVELVAERADG
                     VRFYDDSKGTNVGATVAALAGMDCPVVLIAGGDGKGQDFSPLAPVLAGKARALLLIGR
                     DAGRIEQAVAGCGVAIERVPDLDAAVARANAWAQAGDAVLLSPACASLDMFRNYAHRA
                     EVFVAAVRRLPGVKPR"
     gene            7428..>8583
                     /gene="ftsW"
                     /locus_tag="PKV97_07580"
     CDS             7428..>8583
                     /gene="ftsW"
                     /locus_tag="PKV97_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNB05774.1"
                     /translation="MKFASTLTGLLQREGGAADPARAASGRLGRQGAPARELDLLLIW
                     AAVGLLLLGLVMVYSASIAIAEGSRFTAYQSHYFLLRHAVFLAVGIGLGLMAFQLPMA
                     RWQQLAPALFVAGVVLLVVVLIPGIGREVNGAQRWLSLGPVNLQPSELMKIFAALYAA
                     DYTVRKLDVMGSFVKGFVPMMAVILLVGFLLLREPDFGAFVVITTIAFGVLFLGGVNV
                     RVFVLLAVVAVIGFVILIWTSPYRRDRIFGFMDPWQDAYGKGYQLSHSLIAFGRGEWF
                     GVGLGGSVEKLFYLPEAHTDFLLAVIAEELGFAGVLTVVALFALVVHRAFIIGREAIK
                     LERYFAGLVAQGIGLWIGIQSFINMGVNMGLLPTKGLTLPLMSFGGSGIVA"
BASE COUNT         1182 a         3028 c         3013 g         1360 t
ORIGIN      
        1 ctgatggtgc gcatcgacgc cgtcctcgtc gccctcgccg tcgccagcgc gctcggcgtc
       61 gtcgcctccc agcaccaggc gcgccgcctc catggcgagt acgagcgcga gcaggcgcgc
      121 gcgcaggcgc tcgaggtcga gtggggccag ctccagctcg agcagagtac ctgggcgacg
      181 cacggtcgca tcgagaagct cgcccgcgac cgccttcaca tgcagttgcc gcccgaaggg
      241 caggtgctgg tcgtggagcc tcaatcatga aaaagcaacg tgccgtcacc ttcaatcaca
      301 acccgctgct caagcgcgag ttgccggcct ggcggccgcg catcatcatg ctcgcactga
      361 tgggttgttc gctcgcactg gtcggacgtg cgctctacct gcagggggtc aataacgact
      421 tcctccaggc caagggcgag tcgcgctatg cgcgcgtgct cgaggtcccc gccacccgcg
      481 gccgcatcac cgaccgccac ggcgacattc tcgcggtgag cacgccggtg cgcgcggtct
      541 gggccctgcc cgccgatgcc cggctggagc cggcgcaggt gcgcgagctc gcgcgcctgc
      601 tcgagatgaa cgtcggcgag ctcaacgcca ggctcgccgc ggggcgcgac ttcgtctacc
      661 tcaagcgcca ggtgccgccg gaggtggcgc aggccgtcgc cgacctccgc ctgcccggca
      721 tccaccagga aaaggaatac cgtcgctact acccgggtgg cgaggtgatg gcgcacatcc
      781 tcggcttcac cgatctggaa gatcgcggcc aggaggggat cgagctcgcc ttcgacaagc
      841 gcctgtcggg ccgtgccggc gcgcgccggg tgatcaagga ccgccgcggc cagatcgtcg
      901 aggacgtcga ggcgatccgt ccgccacgcg atggcgacga ggtggcgctg tcgatcgacg
      961 gcaagatcca gtatctcgcc tggtccgccc tgcgcgaggc gatggaagag cacaagccca
     1021 aggccgcggc tgcggtggtg ctcgacgtgc gcaccggcga ggtgctcgcc ctggtcaatg
     1081 cgccgacctt caacccgaac aatcgcgcca acctcaccgg cgcgcaactg cgcaaccgcg
     1141 tcttcaccga cgccttcgag cccggctcgg tgatgaagcc cttcatcgcc gcgctcgcgc
     1201 tcgagcgcgg caaggtcaag ctcaataccc cgatcgatac cggcaacggc cgcatgacga
     1261 tcggcaccgc gaccatctcc gacaccaagc gccacggcgt catcaccatg gcgcaggtgg
     1321 tgcagaagtc ctccaacatc ggcaccgtga agatggcgca gcagctcggc gcagaggaaa
     1381 tgtggcacct cttcgaccag ctcggcttcg gcaccccgct caacctcggc ttccccggcg
     1441 agacttccgg gcggctgcgt cccgccaaga cctggcggcc gatcgagcag gcgacgatgt
     1501 cttacggcca cggcatctcg acgagcctga tccagatggc gcgtgcctac ctcgtgttcg
     1561 cgcgcgaggg cgagctggtg ccgctgtcgc tgacccgggt ggacggcacg ccgagttccg
     1621 ggcgccgcgt gttctcggcc gacaccgcgc gccagatgcg caccgttctg gcgatggcgg
     1681 cgggtcccgg cggcaccgcg atccgcgccc aggtgcccgg ctacacggtg gcgggcaaga
     1741 ccggtaccgc gcacaagctc gaggatggtc gttatgcgcg caagtacatc tcgtccttcg
     1801 tcggcttcgc cccggcgagc gatccgcgca tcgtggtcgc ggtgatggtg gacgagcctt
     1861 ccggaggcca gcactacggc ggcacggtcg ccgcgccggt gttctcgcgc atcaccgaag
     1921 gcacgctgcg cacgctcggc gtcgcccccg acatgccctt gaccccgctc cagctcgctc
     1981 gccgcggcgg cgacgaggca gcgagcgaag gcagcagccg ggagagcatg tgagcgccat
     2041 gcaagccctc gcggtgctcg atcgactgcg tgcgctcggc gtccacgccg acgacatcac
     2101 cgccgattcg cgcaaggccg gcccgggttg cgtgttcgcg gcctggcccg gccagcgcac
     2161 cgatggccgc cgcttcctcg ccgatgcagt cgtgcatggc gccgccgcgc tgttgtggga
     2221 ggatggcgac ggattctgcg cgcccgcgct cgcggtgccc ggtttcggcg tgcgtggcct
     2281 gcgcgagctc gccgggcacc ttgcccacga actccacggc cggccttcgc agtcgctttg
     2341 gctggccggc gtcaccggca ccaacggcaa gaccaccgtc tcgcagatgc tcgcgcgtgc
     2401 gctcggcgaa ctcggcgagc gctgcggcat cgtcggtacg ctcggctgcg ggttccccga
     2461 cgccctcgag gaagggctca acaccacgcc cgacgcgctc gaattgcagc gctggtttgc
     2521 gcgcttccgc aaccaggggg cggtggccgc ggcgatggag gtgtcttcga tcgggctcga
     2581 ccaggggcgg gtcaatggtg cgcagttcga cgtcgcgatc ttcaccaacc tgagtcgcga
     2641 ccacctcgat taccacggca ccatggaagc ctacgccgcg gccaaggcgc gcctgttcga
     2701 gcgcccggag ctgtcggcct gcgtgatcaa catcgacgat ccctggggcc tggcgctggc
     2761 gcgccggctc gtcgtcgagg ggcgcgcggt gatcgcatgc accctctacg ccgccaacgc
     2821 cgacgcggtg cccggcgcgc gcgtgctgcg cgccgatcgc ctgcaggcgg cgcccgcagg
     2881 actgcgcttc accctcgtct gggaggggcg cgaggccgag atcggcgtgc gcatggtggc
     2941 gcacttcaac gtctccaacc tgatcgcggt tgcgggcgcg ctgctggcgc gcggcatcgc
     3001 cttcgacgag ctgccgccgg tgctcggtcg gctggcgccg ccgcaggggc gcatgcagct
     3061 cgtcggcggg gtgtgcgagc cgctcgtggt gatcgactac gcgcattcgc ccgatgccct
     3121 cgccaaggtg ctcgaagcct tgcgcggcac ggtgcatagc cgccgcggcc gcctggtgtg
     3181 cgtgttcggc tgcggcggcg accgcgaccc gggcaagcgc ccgatgatgg gcgagatcgc
     3241 cagcgcgatc gccgatcgcg tggtgatcac cagcgacaac ccgcgttcgg aggatccgca
     3301 gcgcatcgtc gaggccattg ccgccggcgc cggcgccgcg gccgaatgcg tggtcgaccg
     3361 cgcgcaggcg atcacccgcg cggtgggcga ggcgcgggcc gacgacgtcg tgctgatcgc
     3421 aggcaagggc cacgagccgt accaggaaat cctcggccag cgcctgcctt tttcggacct
     3481 ggagcaggcg aagcgcgcgc tcgccgcctg gcatgcaagg gagaatggcc gatgatgagc
     3541 ctgcacgacg ccacgcgcgc actcgcctgt catggtgcgc gggcgaccgg ggaagggcgc
     3601 ttcgacagcg tcggtaccga tacccgcgcg ctcgcgcccg ggcagctctt cgtcgccctg
     3661 cgcggcgagc gcttcgacgg ccacgacttc gtcgccgatg cggcgaaggc gggcgccgcc
     3721 gcggccatgg tcgatgccgc ctggagcgcc ggcaaggccg atgccggcct gcccctgctg
     3781 gtggtggacg acacgcggcg tgcgctcggc acgctcgcgg cggcttggcg ggcgcgtttc
     3841 gcgctgcccc tgatcggcgt caccggcagc aacggcaaga ccacggtgaa agagatgtgt
     3901 gccgcgatcc tgcgcgtgca ggcgagccgc gacggcttcg gcgaggagtc ggtgctcgct
     3961 acgcgcggca acctgaacaa cgacatcggc atgccgctca cgctgctcga actgcgcgac
     4021 ttccatcgcg ccgcggtgat cgagatgggg atgaaccatc ccggcgagat cggctacctg
     4081 agcgcgctcg cccagcccac ggtggccctg gtgaacaacg cccagcgcgc ccacctccag
     4141 ggcatgggct cggtcgacga ggtcgcgcgc gagaagggcg cgatctacga aggcctgggc
     4201 ggggcaggcg tggcggtgat caacgccgac gatccgcacg ccggctactg gcgttcgctc
     4261 aacgccgggc ggccgattgt gacattcggt atcgaccatc cggcggacgt gtccgcgcgg
     4321 tgtaccctgc gcggcctcgg ctcgcggctc gaactcgaca tcccgcaagg gaagatcgat
     4381 ttcgccttgc aggtgccggg cctgcacaac gtgcgcaacg cgctcggcgc tgcggcggcc
     4441 tgcctcgcgg caggggttcc gctcgaggcg gtggccgagg gactggcggg tttcgccggc
     4501 acccgcggtc gcctgcaacg ccgtgccggg ccgcggggcg cgctgatcct ggacgattcg
     4561 tacaacgcca atccggattc ggtgcgcgtc ggcatcgacg tgctcgccgc catgccgggc
     4621 catacctggc tggtgctcgg cgacatgggc gaggtcggcg agaccagcgc ccaggttcac
     4681 gacgaggtcg gtggctatgc gaagagcaag ggcatcgacg gcctcttcgc gctcggcgag
     4741 atgagcgcgg tggcggcgca caacttcggc gagggcgcgc gccatttcac ttcggtggag
     4801 gcgctggtca aggcgctcgt gccgcgcctg gatgcggact cggtggtgct ggtgaagggc
     4861 tcgcgcttca tgcgcatgga aagggtggcg gatgcgcttg cggcagtgca caatccggcc
     4921 ccttcaacgg aaaccggctc ctgacatgtt gttggaactc gctctctggc tcggccagga
     4981 cattcgtggc ttcaacgtct tcggctacat cacgctgcgg acggtgctcg ccgctctcac
     5041 cgcactggcg atctcgctga tcgccggccc cgccgtcatc cgctggctcg cggccaagaa
     5101 gatcggccag gccgtgcgcg acgacggccc caagtcgcac ctcaccaagg ccggcacgcc
     5161 cacgatgggc ggcgcgctga tcctgatcgc gatcggcgtc accatcctgt tgtggggcga
     5221 cctgaagaac gcctacgtgt gggtgaccct gctggtgacc ctgggtttcg gcgccgtggg
     5281 ctgggtggat gactggcgca aggtcgtgca tcgcgatccg aaggggctgg cgagccgctg
     5341 gaaatacctg tggacctcgg cgatcgcgct cgccgcggcg atctatctcg gagtcaccgc
     5401 agacaccccg gccgaaaccg aactgatcgt gcccttcttc aaggcgattg cgtacccgct
     5461 gggcatcttc ggtttcatcg cgctgaccta cttcgtcatc aacggcacca gccacgcggt
     5521 gaacctcacc gacggcctcg acggcctggc gatcatgccg acggtgatgg tggccgcggc
     5581 actggcgatc ttcgcctatg tcgccggtca cgtcggcttc tcgaagtacc tcggcgtgcc
     5641 ctatgtggcg ggcgcgggcg aactggcggt gttctgcggc gcgatctgcg gcgcgggctt
     5701 gggcttcctg tggttcaacg cctacccggc ggaggtcttc atgggcgacg tcggcgcgct
     5761 cgcgctcggt gccgcgctcg gcacgatcgc ggtgatcgtg cgccaggaga tcgtgctctt
     5821 catcatgggc ggcctgttcg tcgccgagac gctgtcggtg atggtgcagg tgctgtactt
     5881 caaggccacc ggcggcaagc gcatcttccg catggcgccg ctgcaccacc attacgaact
     5941 gggtggctgg aaggagaccc aggtcgtggt ccgcttctgg atcatcacca tcatgctggt
     6001 gctgttcggg ctgtcgacgc tgaaactgcg atgagcgcac tcaccggaaa acacgtcctc
     6061 gtcctcgggc tcggcgaatc gggcctggcc atggcgcgct ggtgcgccct gcgcggggcg
     6121 cgcgtgcgcg tggccgacag ccgcgccgtc ccgcccgggg tcgacgcgct gaaggcggac
     6181 gcgccgctgg ccgagatctt catgggcagc ttcggcgacg aggtgctcga cggggtcgat
     6241 ctggtcgcgc tcagccccgg gctcgacccg cgcgtcgggg tcgccgcgca ggcgcgtcgg
     6301 cgcggcctgc cgctcaccgg cgagatgagc ctgctcgcgc aggcgctcga cgagctcggc
     6361 gtgcgcgcgc agacgcgcat cctcgccatc accggcacca acggcaagac caccacgacc
     6421 gcgctcaccg cggcgctggc gcagtcggtc ggcatcgacg cggtcgccgc cggcaacatc
     6481 agcccggcgg cgctcgacgt gctgatggag cgcctggaga tcggcgccgc gctgcccgcg
     6541 tgctgggtgc tggaactctc cagcttccag atcgagacca tgcggggcct ggacgccgag
     6601 gcggcaaccg tgctcaacgt caccgacgac cacctcgacc gctacgccga cctcgaggaa
     6661 tatgccgcca ccaaggcggc gatcttccag ggcgagggcg tgcaggtgct cgctcgcgag
     6721 gacgcgcgcg tcgcggccat ggcgctgccc ggccggcgca cgatccgctt cggcaccgcg
     6781 gcgccggcga gcgaaaccga ctacggtctg gccgacctcg acggccgcgc ctggctggtg
     6841 cgcggcggtg agcgtctgct cgcactcgcc gatctgccgc tcgccggccg ccacaacgct
     6901 gccaacgcgc tcgccgcgct tgcgctctgc gagggtggcc tggggatcgc gccgcaggcg
     6961 ctgatccccg ggctgaaggc cttccgcggc ctgccgcacc gcgtcgagct ggtcgccgag
     7021 cgcgccgacg gcgtgcgctt ctacgacgac tccaagggca ccaacgtcgg cgccacggtg
     7081 gctgcgctcg ccggcatgga ctgcccggtg gtgctgatcg ccggcggtga cggcaagggc
     7141 caggacttct cgccgctcgc cccggtactc gccggcaagg cgcgcgcgct gctgctgatc
     7201 ggtcgcgacg ccggccgcat cgagcaggcg gtggcgggat gcggcgtggc gatcgagcgc
     7261 gtgcccgacc tcgatgccgc ggtggcgcgc gccaatgcct gggcgcaggc gggtgacgcg
     7321 gtcctgctct cgcccgcctg cgccagcctc gacatgttcc gcaactacgc ccaccgcgcc
     7381 gaggtgttcg tcgctgcggt gcgtcgcctg cccggagtga agccgcgatg aagttcgcca
     7441 gcaccctcac cggcctgctc cagcgcgaag gtggcgcggc cgatccggcg cgcgcggcga
     7501 gcgggcgcct cgggcgccag ggggcgccgg cgcgcgaact cgacctgctg ttgatctggg
     7561 ccgcggtcgg cctgctgctg ctcggtctgg tcatggtgta ttcggcatcg atcgcgatcg
     7621 ccgagggcag ccgcttcacg gcctaccagt cgcactattt cctcctgcgc catgcagtct
     7681 tccttgctgt cggcatcggc ctcggcctga tggccttcca gctgccgatg gcgcgctggc
     7741 agcagctcgc cccggcgctc ttcgtcgccg gggtggtatt gctcgtggtg gtgctcatcc
     7801 cgggcatcgg tcgcgaggtc aatggcgcac agcgctggct ctcgctcggg ccggtgaacc
     7861 tgcagccctc cgagctcatg aagatcttcg cggcgctcta tgccgccgac tacacggtgc
     7921 gcaagctcga cgtcatgggc agcttcgtga agggcttcgt gccgatgatg gcggtgatcc
     7981 tgctcgtcgg cttcctgctg ctgcgcgagc ccgacttcgg cgccttcgtc gtgatcacca
     8041 cgatcgcctt cggcgtgctc ttcctcggcg gcgtgaacgt gcgcgtgttc gtgctcctcg
     8101 cggtggtggc ggtgatcggc ttcgtgatcc tgatctggac ctcgccctac cgccgcgatc
     8161 gcatcttcgg cttcatggac ccctggcagg acgcctacgg caagggctac cagctctccc
     8221 attcgctgat cgccttcggg cgcggcgagt ggttcggcgt cggcctcggc ggcagcgtcg
     8281 agaagctctt ctacctgccc gaggcgcaca ccgacttcct gctcgccgtg atcgccgagg
     8341 aactcggctt cgccggcgtg ctgaccgtgg tggcgctgtt cgcgctggtg gtgcaccgcg
     8401 ccttcatcat cgggcgcgag gcgatcaagc tcgagcgcta cttcgcgggc ctggtcgccc
     8461 agggcatcgg cttgtggatc ggcatccagt ccttcatcaa catgggtgtg aacatggggc
     8521 tgctgcccac caagggcctg accctgccgc tgatgagctt cggcggctcg ggcatcgtcg
     8581 cca
//
LOCUS       DAOQNN010000106         8489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4183_length_8489_cov_6.092127, whole genome shotgun sequence.
ACCESSION   DAOQNN010000106 DAOQNN010000000
VERSION     DAOQNN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4183_length_8489_cov_6.092127"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="PKV97_07585"
     CDS             complement(<1..1174)
                     /locus_tag="PKV97_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB05775.1"
                     /translation="MRFRIAVSVTAVLVVLIVAQSLALVRLNEEMEEEFIDGTLDEQL
                     HYSIEHSRRLGTLIGPQTPNMTLYRFAPGETPPQGLRPELAALAVGDHEDWSAGRELH
                     VAVREADGQRYVLIYDESEHRERESAVVATVVIGGLVLTALSFLLVYAIAARLTRGLE
                     TLAERVEGARDGTPFAQPGLDAELLAVARALDRAEARQAAVLARERDFNANLSHELRT
                     PLAGIRSDAEMLLNDPVLSDKARRRAERIIATTDRTTTLAQSLLLLAREARPQALEPI
                     DLSAAVRDAWTRLHPAGAAAAAPSIRIDPQAEVVADPGLLALVLRNLLENALRHGEGR
                     AIEVVLEGRRLEVCDRGPGFGDEDPARCFERFRRGGTKPGHGLGLALVRHICTACGW"
     gene            complement(1218..1886)
                     /locus_tag="PKV97_07590"
     CDS             complement(1218..1886)
                     /locus_tag="PKV97_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB05776.1"
                     /translation="MHLLIVEDDRTIAENLYDYLEARGHQCDYADSIAAASALLARSA
                     FDALVLDRNLPDGDGLALARRLRTEGRATPILVLTARDTLEDKLLGFEAGGDDYLVKP
                     FALQELEVRLLALVRRSAVRAADPVLRYGALEFRAATQALCLHGRPLTLPPKPLRLVA
                     ELLAQPERVFSRRELEIAVWGHEQDSSDNLRSVLHTLRRALGDDAPIQVVNVHGLGYK
                     LVGR"
     gene            complement(1906..2823)
                     /locus_tag="PKV97_07595"
     CDS             complement(1906..2823)
                     /locus_tag="PKV97_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNB05777.1"
                     /translation="MTSITPRQALLLSLAAALSTIAMKTGAWWLTGSIGYLSDALESV
                     VNLAGAGFALWMVSYAQRPADEDHQYGHGKAEYFSAAFEGGLIFIAALIILITAGERL
                     LHPQAIGELGLGTLLSVGASLINFLVARVLLQVGRAHRSLALEADARHLMADVWTTAG
                     VVCGVGLAGATGWSWLDPVVAGAVALNILREGWELMRRSIDGLMDRALSEAEVAAIER
                     TLAEASPPGCFHSKLTTRAAGALHFAHIELCVPGDWTVAEAHAVADALERVVLERTGT
                     HLATHLEPIPPTVPQGGEGHPDRASAARP"
     gene            complement(2828..3280)
                     /locus_tag="PKV97_07600"
     CDS             complement(2828..3280)
                     /locus_tag="PKV97_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05778.1"
                     /translation="MMTKSLISTLLLALAAGIATHARAAGDEPTALSPQACQALHQEL
                     DAMAAHVRAARASGAAQADAPDPYPALAQALEAHLDALKAGLPTPATPRAQAGELLSD
                     MRDALVLVRGATHLEARLLAVQRLEDDRRLYNRVLETLGCSATRPTAL"
     gene            3507..4847
                     /locus_tag="PKV97_07605"
     CDS             3507..4847
                     /locus_tag="PKV97_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNB05779.1"
                     /translation="MKNIRFAYAALLLLLSLLWWMADPLLPAGYEYFALRTVAINYTG
                     VIGIGVMSVGMILALRPVRVEPLLDGLDKTYRLHKWLGITGLVFSVIHWLWAQGTKWA
                     VGWGWLVKPERGPKPPLTDPVESFFRAQRGLAESVGEWAFYAAVVLIVIALAKRFPYR
                     LFFKTHRLLALAYLALVFHSVLLTPFAYWTQPMGIVLAVLMAGGSVAAVVSLSGRVGH
                     RRKAVGEIEELVPFPDNRVLKVAIRFKDRWPGHEAGQFAFVTFSRDEGAHPFTISSGW
                     KGDGRLFFLIKGIGDYTARLPELLRVGDLVTVEGPYGRFDFASGSASGKRRQIWVGGG
                     IGITPFIARMKALAVAPDGREVDLFYSTAEPDQGFIARLQRDAQAAGVRLHVLVSARD
                     GRLDVARICDTVPGWAEADVWFCGPGGFGQSLREGFVARGLPAADFHQELFDMR"
     gene            5023..>5218
                     /locus_tag="PKV97_07610"
     CDS             5023..>5218
                     /locus_tag="PKV97_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05780.1"
                     /translation="MNSQFRAFALLCGLAASAGALAAPSAALLERYAAEARAADPAFA
                     GFSAARGEALRLASQARGAAL"
     assembly_gap    5219..5228
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5229..5476
                     /locus_tag="PKV97_07615"
     CDS             <5229..5476
                     /locus_tag="PKV97_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HNB05781.1"
                     /translation="RHGLGKPDTPACTSCHGENARAAGQTRTGKAIEPMAVSVSATRY
                     GDPAKVEKWFKRNCNEVLGRECSAREKGDWLAWMSTQ"
     gene            5498..5989
                     /locus_tag="PKV97_07620"
     CDS             5498..5989
                     /locus_tag="PKV97_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HNB05782.1"
                     /translation="MNTPVLRPLALGVAAIAVTLMLLGRAQAGDDDFYPPVTDPLTVQ
                     ECASCHIAFPASMLPAASWTRMMGELENHFGDDASVDADTTAAITRYLVEHAGDARGR
                     PGKLLRGLHLGDAPLRITELPKWVREHDEVPRSDWTRKEVGSKANCTACHIDAEKGYF
                     EDD"
     gene            6001..7329
                     /locus_tag="PKV97_07625"
     CDS             6001..7329
                     /locus_tag="PKV97_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNB05783.1"
                     /translation="MKRFLTAFIALVGLAWGVEMLSATGAVASAPGNPWAVREHALTL
                     TGLGSFALMSLAMVLATRPARLERFFGGMDRIYRVHKWAGILAVGFAVLHWLVELSDD
                     LIKTLWGREGRLPKDHGGGLLEAMRDVAEELGEFAIYALLAMLVLTLWKRFPFRIWRY
                     VHKGMPVLYLALAFHAAFLAPLDYWTQPVGVLLAGLLAAGSVASVVVLAGRIGQDRRV
                     GGVVESVRDAGAGVTEVVCRLDEGWRGHRPGQFAFATFDRLEGAHPFTIAGADRGDRR
                     VRFQIKALGDYTRGLAQRLQPGRPMQVEGPYGCFDLPQSLDGREQIWIAGGIGVTPFL
                     AWLEALQARPEQAPDVDFYYSVREHATDPFVARLQALCANLPAVRLHVISGARNERLT
                     AATLRDRPRAGAAPEIWFCGPRGLATSLRKGLRRLGMGGASFHQEAFEMR"
     gene            complement(7326..7790)
                     /locus_tag="PKV97_07630"
     CDS             complement(7326..7790)
                     /locus_tag="PKV97_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HNB05784.1"
                     /translation="MKTTKPGMALLLSVALNLGVLGAVLLDRLGPGAAATPAPPLHRV
                     LALDEAQRARWEAVEAPFLQQFEAAAVRLERHRHALVEALFAESIDPLRIEAERAAIA
                     ELQQDQQRLIIEQLIAEREILDARQRQQLLGLLLAQPPARTAVEDLHARPTR"
     gene            complement(7787..8326)
                     /locus_tag="PKV97_07635"
     CDS             complement(7787..8326)
                     /locus_tag="PKV97_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNB05785.1"
                     /translation="MNSSCPTGSCPPGPCPADEALSALVDAALPSEAGAGLIAHCVGC
                     PRCTRRLAELRALRDAFAALPAIRLEVDLAAQLVDRLQAGATPAARKRRQSPWWVRWA
                     DRLRPPTPWRFGLSPFAGSVAAVGLGLWLGGLLMELEAPRSPPAANTTLAAMSVFGAV
                     PPGNLCPRAGACGASGSTR"
     gene            complement(8319..8471)
                     /locus_tag="PKV97_07640"
     CDS             complement(8319..8471)
                     /locus_tag="PKV97_07640"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNB05786.1"
                     /translation="MLLLREVEGLAYGELASVLGIAEGTVKSRLARARSTLLEHFDPH
                     TGASHE"
BASE COUNT         1164 a         2984 c         3111 g         1220 t
ORIGIN      
        1 cccagccgca ggcggtgcag atgtgccgca ccagcgccag gccgaggccg tgtcccggct
       61 tggtcccgcc gcgccggaag cgctcgaagc agcgcgccgg gtcctcgtcg ccgaagccgg
      121 ggccacgatc gcacacctcg aggcgacgcc cttccagcac cacctcgatc gcgcgcccct
      181 cgccatgacg cagggcgttc tccagcaggt tgcgcagcac gagcgccagc aggccggggt
      241 cggcgacgac ctcggcctgg gggtcgatac ggatgctggg cgctgcggcc gcagcgccgg
      301 ccgggtgcaa gcgcgtccag gcgtcgcgca ccgccgcgct caggtcgatc ggttcgagcg
      361 cctgcggccg cgcctcgcgg gcgagcagca gcaggctttg ggcgagcgtg gtggtgcggt
      421 cggtggtggc gatgatgcgc tcggcacggc ggcgggcctt gtcggacagg accggatcgt
      481 tcagcagcat ctcggcatcg ctgcggatac cggcgagcgg ggtgcgcagc tcgtgcgaca
      541 gattggcgtt gaaatcgcgc tcgcgcgcca gcacggcggc ctggcgcgcc tcggcgcggt
      601 cgagcgcgcg cgccacggcg aggagttcgg cgtcgagtcc gggctgagcg aagggcgtgc
      661 cgtcgcgcgc gccctccacg cgctcggcga gcgtctccag cccgcgcgtg agccgtgcgg
      721 cgatggcgta cacgaggagg aaggacaagg cggtcagcac caggccgcca attaccaccg
      781 tggcgaccac ggccgactcg cgctcgcgat gttcggattc gtcgtagatc agcacgtagc
      841 gctggccgtc ggcctcgcgc accgcgacgt gcagctcgcg cccggcggac cagtcctcat
      901 ggtcgccgac cgccagcgcg gccagctcgg ggcggagccc ttgcggcggc gtttcgccgg
      961 gggcgaagcg gtagagcgtc atgttcggcg tctgcggccc gatcagggtg ccgaggcggc
     1021 ggctgtgctc gatgctgtag tgcagctgct cgtcgagcgt gccgtcgatg aactcctcct
     1081 ccatctcttc gttgagccgc accagcgcga gcgactgcgc cacgatgagc acgaccagca
     1141 cggccgtgac cgacaccgcg atgcgaaagc gcatgccgcc cggtagccag cgccggccac
     1201 cgcggcgcgc gccgccctca gcggccgacg agcttgtagc cgaggccatg gacattcacc
     1261 acctggatgg gcgcgtcgtc gccgagggcg cgacgcaggg tgtgcagcac gctgcgcagg
     1321 ttgtcgctgc tgtcctgctc gtggccccac accgcgatct cgagctcgcg gcgcgagaac
     1381 acgcgctcgg gctgggcgag cagctcggca accaggcgca ggggcttcgg cggcagcgtg
     1441 agcgggcggc cgtgcaggca cagcgcctgg gtcgcggccc ggaactcgag cgcgccgtag
     1501 cgcagcaccg ggtcggccgc ccgcacggcg ctgcgccgca ccagcgccag caggcgcacc
     1561 tcgagctcct gcagcgcgaa gggcttgacc aggtagtcgt cgccgccggc ctcgaagccg
     1621 agcagcttgt cttccagcgt gtcgcgcgcg gtcagcacca ggatcggcgt cgcgcgccct
     1681 tcggtgcgca gccggcgtgc gagcgcgagg ccgtcgccgt cgggcaggtt gcggtcgagc
     1741 accagcgcgt cgaacgcgct gcgcgcgagc agggccgagg ccgccgcgat cgagtcggcg
     1801 tagtcgcact ggtggccgcg cgcctcgagg tagtcgtaga ggttctcggc gatggtgcgg
     1861 tcgtcttcga cgatgagcag gtgcatcggg agggtggggc ctggtctagg gccgtgcggc
     1921 ggacgcgcgg tccgggtgcc cttccccgcc ctgtgggacg gtcggcggga tgggctcgag
     1981 gtgggtggcg aggtgggtgc cggtgcgctc gagcacgacg cgttcgagcg catcggccac
     2041 ggcgtgggcc tcggcgacgg tccagtcgcc gggcacgcac agctcgatgt gggcgaagtg
     2101 cagcgcgccc gcggcccgtg tggtgagctt gctgtggaag catcccgggg gcgaggcctc
     2161 ggccagcgtg cgctcgatcg ccgccacctc ggcctcggac agcgcgcggt ccatgaggcc
     2221 gtcgatcgag cgccgcatca gctcccagcc ctcgcgcagg atgttgagcg cgaccgcgcc
     2281 ggcaaccacc ggatcgagcc agctccagcc ggtggcgccc gccaggccga cgccgcagac
     2341 cacgcccgcg gtggtccaca cgtcggccat caggtggcgc gcgtcggcct cgagcgcgag
     2401 cgagcggtgc gcgcggccga cctgcagcag cacgcgcgca acgagaaagt tgatcagact
     2461 cgcgccgacg gacagcaagg tgccgagccc gagttcgccg atcgcctgcg gatggagcag
     2521 gcgttccccg gcggtgatca ggatgatcag cgcggcgatg aagatcagcc cgccctcgaa
     2581 cgcggccgag aagtattcgg ccttgccatg accgtactgg tgatcctcgt ccgccgggcg
     2641 ctgcgcgtag gacaccatcc acagcgcaaa acccgcgccc gccaggttga ccaccgattc
     2701 gagcgcatcc gacagatagc cgatcgagcc ggtcagccac caggcgccgg tcttcattgc
     2761 gatggtgctc agcgctgcgg cgagcgagag cagcagggcc tggcgcggcg tgatcgaggt
     2821 catcggttca aagtgcggtg ggtcgagtgg cggagcaccc cagggtctcc aggacgcgat
     2881 tgtagagccg tcgatcgtcc tcgaggcgct gcaccgcgag caggcgggct tcgaggtggg
     2941 tggcgccgcg caccaggacc agggcgtcac gcatgtcgct caacaactcg ccagcctgcg
     3001 cgcgcggtgt cgccggcgtc ggcaggccgg ccttcaaggc atcgaggtgg gcctccagtg
     3061 cctgcgccag cgccgggtag ggatcgggcg cgtccgcctg tgcggcgccg ctcgcgcgcg
     3121 ccgcgcgcac gtgggccgcc atggcatcga gttcctggtg cagcgcctgg caggcctgcg
     3181 gcgacagcgc cgtcggttcg tccccggcgg cgcgggcgtg ggtggcgatc ccggcggcaa
     3241 gcgccagcag cagcgtcgaa atcaagctct tggtcatcat tggaaaggct ccgtggtcgg
     3301 gacaacccgc agcatggggc cgcgatcctt aagcggacct gaatggccga ccccggggcg
     3361 gcgtcgccat gcgatgcgca ccggccttgt ccggcgacaa ggccggcggc gggctcggcg
     3421 cggatactag cgggaggtgg cgctcgacga ggcacgctgg ccgcgcctcg gtgcgggcgc
     3481 cgaaatgcga tcgaacggag agcatgatga agaacatccg gtttgcgtac gcggcgctcc
     3541 tgctgctgct cagcctgctg tggtggatgg ccgatccgct gctgcccgcc ggctacgagt
     3601 acttcgccct gcgcacggtg gcgatcaact acaccggcgt gatcggcatc ggcgtcatga
     3661 gcgtggggat gatcctggcg ctgcgcccgg tgcgggtcga gccgctgctc gacgggctgg
     3721 acaagaccta tcgcctgcac aagtggctgg gcatcaccgg cctggtgttc tcggtcatcc
     3781 actggctgtg ggcgcagggc accaagtggg cggtgggctg gggctggctg gtcaagcccg
     3841 agcgggggcc gaagccgccg ctcaccgacc cggtcgagag cttcttccgc gcacagcgcg
     3901 gcctggcgga atcggtcggc gagtgggcct tctacgcggc ggtggtgctg atcgtgatcg
     3961 cgctcgccaa gcgctttccg taccggctgt tcttcaagac ccaccgcctg ctggccctcg
     4021 cctacctggc gctggtcttc cactcggtgc tgctgacgcc gttcgcctac tggacgcagc
     4081 cgatgggcat cgtgctcgcc gtgctgatgg ccggcggcag cgtcgccgcc gtggtgtccc
     4141 tgtccggccg tgtcggccac cggcgcaagg cggtcggcga gatcgaggag ctggtgccct
     4201 tccccgacaa ccgcgtgctc aaggtggcga tccgcttcaa ggaccgctgg ccggggcacg
     4261 aggctggcca gttcgccttc gtgaccttct ccagggacga aggcgcgcat cccttcacca
     4321 tctcctcggg ctggaagggg gacggccgcc tgttcttcct gatcaagggg atcggcgact
     4381 acaccgcgcg cctgcccgaa ctgctccgcg tcggcgacct cgtcaccgtc gaaggcccct
     4441 atggccgctt cgatttcgcc agcggcagcg ccagcggcaa gcgccggcag atctgggtcg
     4501 gcggcggcat cggcatcacc cccttcatcg cccgcatgaa ggcgctcgcg gtggcgcccg
     4561 acgggcgcga ggtcgatctg ttctacagca cggccgaacc cgaccagggc ttcatcgccc
     4621 gcctgcagcg cgatgcgcag gcggcggggg tgaggctgca cgtgctggtg agcgcgcgcg
     4681 acggccgcct cgacgtggcg cgcatctgcg acaccgtgcc gggatgggcc gaagccgacg
     4741 tctggttctg cggaccgggc ggcttcggcc agtccctgcg cgagggcttc gtcgcccggg
     4801 gcctgccggc ggccgacttc caccaggaac tcttcgacat gcgctgagcg cgggcagcgc
     4861 gcgggcgcat cggcgcccgc ccccgccgct ccttggcgca cgcgctttct ccgttcaggt
     4921 tcgattcagt ggcgcgcttg cacactgggc tcgcgattcc ggacgactgg catccgcgat
     4981 gcccgtccgg tcgagtcctc agcccggatc aaggaacgaa ccatgaacag ccaattccgc
     5041 gcgtttgcgc tgctgtgcgg cctcgccgcc agtgccgggg cgctcgccgc cccgagcgcc
     5101 gccctcctgg agcgctacgc cgccgaggcg cgcgccgccg accccgcctt cgccggtttt
     5161 tccgccgcac gcggcgaagc cctgcgtcta gcttctcaag cccgaggagc tgctctagnn
     5221 nnnnnnnncc cgccacggcc tcggcaagcc cgacacgccc gcctgcacct cctgccatgg
     5281 cgagaacgcg cgcgcagccg gccagactcg caccggcaag gccatcgagc cgatggcggt
     5341 gtcggtgtcg gccacacgct acggcgaccc ggcgaaggtc gaaaaatggt tcaagcgcaa
     5401 ctgcaacgag gtgctcggcc gcgagtgcag cgcacgcgag aagggcgact ggctcgcctg
     5461 gatgagcacc cagtaagcgc cgccccggag actcgccatg aacacccctg tccttcgccc
     5521 cctcgccctc ggtgtcgccg cgatcgccgt gaccctcatg ctgctcggcc gcgcccaggc
     5581 cggcgacgac gacttctatc cgccggtcac cgacccgctc accgtgcagg agtgcgcgag
     5641 ctgccacatc gcgtttccgg cgtcgatgct gcccgcggcg tcgtggacac gcatgatggg
     5701 cgaactggag aatcatttcg gcgacgacgc cagcgtcgat gccgacacca ccgccgcgat
     5761 cacccgctac ctggtcgaac acgccggcga cgcgcgcgga cggccgggca agctgctgcg
     5821 cggcctgcac ctcggcgatg cgccgctgcg catcaccgag ctgccgaagt gggtgcgcga
     5881 gcatgacgag gtcccgcgct ccgactggac gcgcaaggag gtgggcagca aggccaactg
     5941 caccgcgtgc cacatcgacg ccgagaaagg ctatttcgag gacgattgag gagcccgatc
     6001 atgaaacgct ttctgactgc cttcatcgcc ctggtcggcc tggcctgggg cgtggaaatg
     6061 ctgtcggcga cgggcgccgt cgccagcgcg ccgggcaatc cctgggcggt gcgcgagcac
     6121 gcgctgacgc tcaccggcct ggggtccttc gcgctgatgt cgcttgcgat ggtgctcgcc
     6181 acccgtccgg cccggctgga gcgttttttc ggcggcatgg accgcatcta ccgtgtgcac
     6241 aaatgggccg gcatcctcgc ggtcggcttc gcggtgctgc actggctggt cgagctctcc
     6301 gacgacctca tcaagacgct gtgggggcgc gaggggcgcc tgcccaagga tcacggcggc
     6361 ggcctgctcg aagccatgcg cgacgtcgcc gaggagctgg gcgagttcgc gatctacgcg
     6421 ctgctggcga tgctggtgct gacgctgtgg aagcgcttcc cgttccggat ctggcgttac
     6481 gttcacaagg ggatgccggt gctgtacctg gcgctcgcct tccatgccgc cttcctcgcg
     6541 ccgctcgact actggacgca accggtcggc gtgctgctgg cgggcctgct cgctgccggc
     6601 agcgtcgcca gcgtcgtggt gctggctggc cgcatcggcc aggaccgccg cgtcggcggc
     6661 gtggtggagt cggtccgcga cgccggcgcg ggcgtcaccg aggtggtctg ccggctcgac
     6721 gagggctggc gcggacaccg gccggggcag ttcgccttcg cgaccttcga tcgtctggag
     6781 ggcgcgcatc ccttcacgat cgccggcgcc gaccgcggcg accgtcgggt gcgcttccag
     6841 atcaaggccc tgggtgacta cacccgcggc ctcgcgcagc gcctgcagcc gggtcggccc
     6901 atgcaggtcg aaggccccta tggatgcttc gatctgccgc agtcgctgga cgggcgggag
     6961 cagatctgga tcgccggcgg catcggcgtg acccccttcc tcgcctggct cgaggcgctg
     7021 caggcgaggc cggagcaggc gcccgacgtc gatttctact acagcgtgcg cgagcatgcg
     7081 accgacccct tcgtcgcccg cctccaggcc ttgtgcgcaa acctgcccgc ggtgcgcctg
     7141 cacgtgatca gcggcgcccg caacgagcgc ctcaccgcgg cgacgctgcg cgaccgcccg
     7201 cgcgcgggcg cggcgccgga gatctggttc tgcgggccgc gcggcctcgc caccagcctg
     7261 cgcaagggct tgcgccgcct cggcatgggc ggcgcgagct tccaccagga ggcgttcgag
     7321 atgcgctagc gcgtcgggcg cgcgtgcagg tcctcgaccg cggtgcgtgc cggcggctgg
     7381 gcaaggagca ggccgagcag ctgctggcgc tggcgcgcgt cgagaatctc gcgctcggcg
     7441 atcagctgct cgatgatcag gcgctgctgg tcctgctgca gctcggcgat cgccgcacgc
     7501 tcggcttcga tgcgcagcgg gtcgatcgac tcggcgaaga gcgcttcgac gagggcgtgg
     7561 cggtgacgct ccaggcggac ggcggccgcc tcgaactgct gcaggaaggg cgcttcgacc
     7621 gcctcccagc gcgcgcgctg cgcctcgtcg agcgccaata cccgatgaag gggtggcgcc
     7681 ggcgtggcgg cggcacccgg cccgagccga tccagcagca cggcgccgag cacgccgagg
     7741 ttgagtgcga ccgacagcag cagcgccatt ccaggcttgg tggtcttcat cgcgtgctcc
     7801 cgcttgcgcc gcaggccccg gcccgcggac agagatttcc cggtgggacg gcgccgaaca
     7861 cgctcatggc ggccagcgtc gtgttcgctg caggcggcga ccgcggcgct tcgagctcca
     7921 tcagcagtcc gcccagccac agcccgagcc cgacggctgc gacggaaccg gcgaacggag
     7981 agaggccgaa gcgccagggc gtcggcgggc ggagtcgatc cgcccagcgc acccaccagg
     8041 ggctctgcct gcgcttgcgc gcggccggcg tcgcgcccgc ctgcaggcgg tcgaccagct
     8101 gtgctgccag atcgacctcg aggcggatgg cggggagcgc cgcaaaggcg tcgcgcaggg
     8161 cgcgcaattc ggcgagccgg cgcgtgcagc gcgggcagcc cacgcagtgc gcgatcaatc
     8221 cggcgccggc ctcggagggc agggccgcat cgacgagggc cgacagcgct tcgtcggcgg
     8281 ggcacggccc gggagggcag gaccctgtgg gacaagagct attcatggga ggctccggtg
     8341 tgcgggtcga aatgttcgag caaggtgctg cgtgcgcgtg cgaggcggga tttcaccgtg
     8401 ccctcggcaa tgccgaggac gctcgccagc tcgccgtagg ccaggccctc gacctcgcgc
     8461 agcagcagga tctcgcggtg ctcggcggc
//
LOCUS       DAOQNN010000107         8450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4213_length_8450_cov_10.528410, whole genome shotgun sequence.
ACCESSION   DAOQNN010000107 DAOQNN010000000
VERSION     DAOQNN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4213_length_8450_cov_10.528410"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PKV97_07645"
     CDS             complement(<1..932)
                     /locus_tag="PKV97_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB05787.1"
                     /translation="MSRSKVILVIDDDRGFRGLLTAQLADMGYDTTGAASWAEASACL
                     KEIEPDLILLDMKLPDTTPELAVPELAAEYPVIVLTGYGSIRSAVSVIHAGALDYLTK
                     PISLAELELTIARVLDNAEMRRSNALYKRQLAARQGSGLIGNSAAILALESLIDAVAP
                     TNATVLIHGESGSGKEVVASAIHQRSERKNRELVTVDATTLSETLFESELFGHEKGAF
                     TGADRQKKGLIEAAEGGTLFLDEIGEVSLANQARLLRLFESGTFRRVGGTKTLRADVR
                     FIVATHRNLGLMVREGRFRSDLFFRLSSFVLTSPP"
     gene            complement(929..2902)
                     /locus_tag="PKV97_07650"
     CDS             complement(929..2902)
                     /locus_tag="PKV97_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB05788.1"
                     /translation="MREPASLHRRSTHGACRAGGSACRCERRAALSPNGSLRVPIRVV
                     AARQDSLARQMLWRILIVASAASVAFATVFVALYLDQLKATRADASLNLNRMLATTWE
                     NAMLKRDVEGLREIVSSLGALRDIRDVLVLAPNGEVRFASNPVRLGMQLPGLVASTAG
                     GMATSRFEHGIEDEEVLRSINPVPNRTECVPCHGPVSSSPINGVLVIDYDATPVRQQA
                     LASAALFAAAGGLVIVLTIMAVWWLLRRRVLNPLAHLDRAAGALAAGDLSARVEVAHL
                     DEIGRSAQRFNTMADALQRELGRHAAHRKYLQDLIDGLPDGLRVIRKSDFIVVSANRA
                     FGEQLGLSAMEGQPCYISSHGRSSPCPPTMVRCPVAELQAHGQVLKCHHRHRRASGEE
                     FAIEVHAGLIDVETEGGRERYIVESVRDVSRVASISQEHRMTELGLLAAGIAHEIHNP
                     LGSLRLGLEAMERRLRGRPERDDRIDDYLRTMDEEIDRCLQVTQRLLMLSRPPQARVQ
                     PVDVNPVLADTSCLLAYDAENRQIEHILEADKAAPRVWADDADLRMLMINLVQNAHHA
                     MPSGGQVTLRSHIIGDEVLIEVEDTGVGIPEDDVPHLFEPFFSRRADGEPGTGLGLSI
                     CRSIVDHYGGRIAVESRPGEGARFRVSLPRVLE"
     gene            complement(2809..3408)
                     /locus_tag="PKV97_07655"
     CDS             complement(2809..3408)
                     /locus_tag="PKV97_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNB05789.1"
                     /translation="MLESLRAVGFPANLALLPGDAMAREAWRALSAALREIPVQPDAA
                     ALDELAAEFAAIYLTGAYRLSPCESYWLDQDHLLYQGPMFELRQRYAEAGLRVKDWRK
                     RPEDHVTHQLGFVARLLSAARGEEDWKQLASFLDQHLLRWIHDFARAVHARTGASFYA
                     ALAVLTATWCASLRACIDVPPMAHAELAEAHADANDVQH"
     gene            complement(3503..4171)
                     /locus_tag="PKV97_07660"
     CDS             complement(3503..4171)
                     /locus_tag="PKV97_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB05790.1"
                     /translation="MSKKLDMDEVRSALERQAAQTYRPVKPAVQLGFVHNNVDCIGCR
                     ACEIACKDKNGLAPGPRFRRVMYVEGGHYPDVFAYKVNMSCNHCADPACLPTCPTGAI
                     WKRADNGVVDIDSTLCIGCRRCEAACPYGAPQWDPTEHIVKKCNMCIDELEAGRKPYC
                     VMACMMRVLDIGPIDALRAGTYPTTAVGPGDRVTGQVKDMANPALTHPSIVFVPHRRG
                     RVDE"
     gene            complement(4182..6755)
                     /locus_tag="PKV97_07665"
     CDS             complement(4182..6755)
                     /locus_tag="PKV97_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNB05791.1"
                     /translation="MNKDTLAAPINPARRDFLKTTAAGAAAAAASSFVELASTGKEAS
                     AFAYEPYYTDDQLTTMVTSCAHNCGSRHMLVAHKKGDVIVRLSSDDGRYQAGGAFGFE
                     TEAVPQLRACLRGRSYRARLYSAERLLYPMVRVGERGEGRFKRVSWDEALDRIAKKMI
                     ELKDTYGPTAILDQSYAGASYGVLHKSDQIEGLLARFLGMFGCRTSSWSVPSYQGTTF
                     SSRTTFGTIEDGNEDDAFAHSKLIIMWGWNPAYTFHGGNTFHYMRLAKQRGCKFVVVD
                     PQYTDSASAYDAWWIPIRPNTDAAMLAAMAHYIFVNELQDQAFINRFCLGVDAGTMPD
                     WARGHENFKDYILGTYDGQPKTPEWAAEICGVAADDIRKLADMYARTKPAALKASWSP
                     GRNAYGEQYNRMAAALQAMTGNIGILGGCAEGVGKGWHAEAVAYPYDEYANVWSASIK
                     SDRWAHCVLNYPDVRREEIGLWPRADQFDGVIPNIKAIWWHGSDWFNQLTNINKEIEA
                     IRKLELVVCADSTITPSGLWADILLPVATHFERHDVALPWYKGHYYIHRPKVIAPLGE
                     SKSDFQIFTELAYRLEALDPARLSGFGKRYNPKADRSYFENDDPVDEAYLVDWWHRVQ
                     SHQGVTMSWEEFKQRGVYKFEFAQPHVAFRDQIEKGVPFNTPSGRIEIFSTTLAQVSD
                     WTKTQYGYPIPAIPKWIEPFEWLGDTGKAAKHPFHLISPHPRWRTHSIFNNIPWLRET
                     YQQEVTIAARDAERLGIRTGDVVEVWNERGKVVVPAYVTERCMPGVLVLHEGAWMDLD
                     DKGVDRAGNPDFLTDDQPSPAGAFAYNTVLADVKKTTLEHRPGWDSLATARSIVFRRD
                     Y"
     gene            complement(6773..7855)
                     /locus_tag="PKV97_07670"
     CDS             complement(6773..7855)
                     /locus_tag="PKV97_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05792.1"
                     /translation="MRLPVLILLLAGINAAMADAPAYPVAGLQPDARPAGAPVIHQHT
                     TSEAAKTRLQRGIVLPAPATLAVMRDQEAWYTPFDQPGMLGYYDLRGWHAAAPKKTAA
                     KKPAKPGGSAERPGEHMRATYSPLHFKPAIETATDAQCLACHADVLEDKPLARSPAGV
                     AAKGSLAWYQQVSTYQGEQDSFHRRHLVTPLAKQLMDLKCTTCHQGHDPRDEAPGTSA
                     NTAAQGSGDFTLRKAVNTEAICLKCHGQPTDYTIMGLPGPWHEVRIDMQNDCLACHVA
                     FRTNRHQVNYLKPAAIEAAAEAGREHMAGGDVCFGCHGGRPWYRIAYPFARNAWPGMA
                     DEVPEWAKDRPTRSEARFLIDKPAAK"
     gene            complement(7929..>8450)
                     /locus_tag="PKV97_07675"
     CDS             complement(7929..>8450)
                     /locus_tag="PKV97_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05793.1"
                     /translation="SKHSINVDMGIAMKKLIVVAASALTLAMAGSVFAQQQGMGGMSG
                     MGGMGGMSGMGGMGGMSGMGGMGGMSGMGGMAGMGGMGGMSGMGGMSGMGGMGGMSGM
                     AGMGGMSGMGGTTVIINIPAGMAGMGGMSGMSGMGGMSGMGGMSGMGGMSGMGGMSGM
                     GGMSGMAADAPKK"
BASE COUNT         1386 a         2743 c         2818 g         1503 t
ORIGIN      
        1 ggtggcgagg tgagcacgaa gctggagaga cggaagaaga ggtcgctgcg aaagcgcccc
       61 tcgcgcacca tgagcccgag gttgcggtga gtggcgacga tgaagcggac gtcggcccga
      121 agcgtcttgg tgccgcctac acggcggaac gtgccgctct cgaagaggcg tagcaggcgg
      181 gcctggttgg cgaggctgac ctcgccgatc tcgtcgagga acagggtgcc gccttcggcg
      241 gcctcgatca ggcccttctt ctgccggtcg gcgccggtaa atgcaccctt ctcgtggccg
      301 aagagctcgg actcgaacag cgtctcggac aaggtcgtgg cgtcgacggt caccagctcg
      361 cggttcttgc gctcgctgcg ctgatggatc gcgctggcga cgacctcctt tccgctgccc
      421 gattcaccat ggatcagcac ggtggcgttg gtcggggcga ccgcgtcgat gaggctctcg
      481 agcgccagga tcgccgccga gtttccgatc agccccgatc cctggcgtgc cgccagctga
      541 cgcttgtaga gcgcattcga acgacgcatc tcggcgttgt cgaggacgcg ggcgatggtg
      601 agttcgagtt cggcgagact gatgggcttg gtcagatagt cgagggcgcc ggcgtggatg
      661 accgagaccg cgctacgaat actgccgtag ccagtaagca cgatgacggg atactcggca
      721 gcgagctcgg gcactgcgag ctcgggcgtg gtgtcgggca gcttcatgtc gagcaagatg
      781 aggtcgggct cgatctcctt gaggcaggcg ctcgcctcgg cccagcttgc agcacccgtg
      841 gtgtcgtagc ccatgtcggc gagctgggcg gtcagcaggc cgcgaaagcc gcggtcgtca
      901 tcgatgacga ggatgacctt gctgcgactc attcgagcac ccggggcaga gacaccctga
      961 agcgcgcgcc ctcgcccggg cggctctcga ccgcgatgcg gccaccgtaa tggtccacga
     1021 tgctgcggca gatgctgagc cccaacccgg ttcccggttc gccatccgcg cgccgactga
     1081 agaaaggctc gaagagatgc ggaacgtcgt cttcggggat cccaaccccg gtgtcctcca
     1141 cctcgatcag cacctcgtct ccgatgatgt ggctgcgcag ggtcacctgc cctcccgagg
     1201 gcatcgcatg gtgggcgttc tgcacgaggt tgatcataag catccgcagg tcggcgtcgt
     1261 ccgcccacac ccgcggtgcc gccttgtcgg cctcaaggat gtgctcgatc tggcgattct
     1321 ccgcatcgta ggcgagcagg caggaggtgt ccgcaagcac cggattgaca tccaccggct
     1381 ggacacgagc ctggggcggg cgggacagca tcagtagccg ctgggtgacc tgcaggcagc
     1441 ggtcaatctc ctcatccatc gtgcgcaggt agtcgtcgat gcgatcgtcg cgctcggggc
     1501 gtccgcgcaa gcggcgctcc atcgcctcga ggccaaggcg cagtgaaccg agcgggttgt
     1561 ggatctcgtg cgcaatgccg gctgcgagca agccgagttc ggtcatcctg tgctcctgcg
     1621 agatgcttgc cacgcggctg acatcgcgga ccgattcgac gatgtagcgc tcgcgcccac
     1681 cctccgtctc gacatcgatg aggccggcgt gtacctcgat cgcgaactcc tcgccgctcg
     1741 cacgccgatg acggtggtgg cacttcagga cctggccatg cgcctgcagc tcggcaacag
     1801 ggcaccgcac catcgtgggc ggacaggggc tgctgcggcc gtgactggag atgtaacagg
     1861 gctggccctc catcgcggac aagccgagtt gctcgccgaa cgcccggttt gcgctaacca
     1921 cgatgaagtc ggacttgcgg atgacgcgca gcccgtccgg caggccatcg atgaggtcct
     1981 gcagatattt gcggtgcgcg gcgtggcggc cgagttcgcg ctggagcgcg tcggccatcg
     2041 tattgaagcg ctgtgcgctg cgcccgatct cgtcgaggtg ggcgacctcg acgcgcgcgc
     2101 tcagatcgcc agcggcgagc gcgcctgcgg cgcgatcgag gtgcgcaagc gggttgagca
     2161 cccgtctgcg caacaaccac cataccgcca tgatcgtgag cacgatgacc agcccgcccg
     2221 cagccgcgaa cagggcggcg ctcgcaagcg cctgctggcg caccggggtg gcgtcgtagt
     2281 cgatcacgag gacgccgttg atcggcgacg acgacaccgg gccatggcac ggcacgcact
     2341 ctgtacggtt gggcaccgga ttgatcgaac gcagcacttc ctcatcctcg atgccatgct
     2401 cgaaccggct cgtggccatt ccgcccgctg tcgaggccac gagcccgggg agctgcattc
     2461 cgagccggac cgggttggag gcgaagcgca cttcaccatt gggggcgagt accaggacgt
     2521 cacggatatc gcgcagcgca cccaggctgg agacgatctc gcgcaagccc tcgacgtcgc
     2581 gcttgagcat cgcgttttcc caggtggtcg cgagcatccg gttcaggttg agcgaggcat
     2641 cggcgcgcgt cgccttgagt tgatcgaggt aaagcgcgac gaagaccgtc gcgaacgcga
     2701 ccgaggcggc gctcgcaaca atcaggattc tccagagcat ctggcgcgcc aggctgtcct
     2761 ggcgggccgc aacgacacgt atcggcactc gcaagcttcc gttcgggctc agtgctgcac
     2821 gtcgttcgca tcggcatgcg cttccgccag ctcggcatgc gccatgggtg gaacgtcgat
     2881 gcaggctcgc aggctcgcgc accaggtcgc ggtgagcacc gcgagtgcag catagaaaga
     2941 ggcgcccgtc cgcgcgtgca ccgcacgcgc gaagtcgtgg atccagcgca acaggtgctg
     3001 gtcgaggaag ctggcgagct gtttccagtc ctcttcgccc cgcgccgccg agagcagcct
     3061 tgcgacgaag ccgagctgat gggtcacgtg gtcttccgga cgcttgcgcc agtccttgac
     3121 acgcagcccc gcctcggcat agcgctggcg tagctcgaac atcggccctt ggtagagcag
     3181 atggtcctga tcgagccagt aggattcgca cggcgacagc cggtacgcgc cggtgagata
     3241 gatcgcggcg aactccgcgg cgagctcatc cagcgcggct gcgtccggtt gcaccgggat
     3301 ctcgcgcagc gcagcagaca gtgcgcgcca tgcctccctg gccatcgcat cgccgggcag
     3361 cagcgcgaga ttggccggaa agccgaccgc gcgcaggctc tcgagcacgg ccgcatcgag
     3421 ctcgctggca tgcaggaaac tcagcatgtc ggcgtcggcg gcgagcgctt gcgccgcctc
     3481 ctgccctggc cagcgcgcgt gctcactcat ctacgcgacc tcggcgatgt ggcacgaaaa
     3541 cgatcgatgg gtgggtgagc gcggggttcg ccatgtcctt gacctgtcca gtgaccctgt
     3601 ctcccgggcc gaccgcggtc gtcgggtacg tccccgcccg cagcgcgtcg atcggaccga
     3661 tatccagtac ccgcatcata caggccatca cacagtaagg cttacgcccg gcctcgagct
     3721 cgtcgatgca catgttgcat ttcttcacga tgtgctcggt cgggtcccac tgcggcgcgc
     3781 cgtaggggca ggccgcctcg cagcgccgac acccgatgca gagggtggag tcgatgtcga
     3841 cgacgccgtt gtcggcccgc ttccagatcg caccggtcgg gcaggtgggc aggcaggcag
     3901 gatcggcgca atggttgcag ctcatgttca ccttgtaggc aaacacgtcg gggtaatggc
     3961 cgccttcgac atacatcacg cgccggaagc gcggcccggg ggcgagaccg ttcttgtcct
     4021 tgcaggcgat ctcgcaggcg cgacaaccga tgcagtcgac gttgttatgc acgaagccca
     4081 gctgtaccgc tggcttgacg ggtctgtagg tctgggccgc ctggcgttcg agggccgagc
     4141 ggacttcgtc catatcgagc ttcttgctca tgagcgcatc ctcaatagtc gcgtcggaaa
     4201 acgatcgagc gcgcggtagc gagcgaatcc cagcctgggc gatgctccag cgtcgtcttc
     4261 ttcacgtcgg ccagcacggt gttgtaggcg aatgccccgg caggcgaggg ttggtcgtcg
     4321 gtcagaaaat ccgggttacc cgcgcgatcg acgcccttgt cgtcgagatc catccacgcg
     4381 ccctcatgca gcacgaggac gcccggcatg cagcgttcgg tcacataggc cggcaccacc
     4441 accttgccgc gttcgttcca cacctccact acgtcaccgg tgcggatgcc gagccgctcc
     4501 gcatcgcgcg ctgcgatggt gacctcttgc tggtaggtct cccgcagcca gggaatgttg
     4561 ttgaagatgc tgtgggtgcg ccagcgcggg tgcggagaga tcaggtggaa gggatgcttc
     4621 gcggccttgc cggtatcgcc cagccactcg aagggctcga tccacttcgg gatcgccggg
     4681 atcgggtaac cgtactgggt cttggtccag tcgctgacct gggccagcgt ggtggagaag
     4741 atctcgatcc gccccgacgg ggtgttgaac ggcacccctt tttcgatctg gtcgcggaac
     4801 gctacgtgcg gttgcgcgaa ttcgaacttg tataccccgc gctgcttgaa ttcttcccag
     4861 ctcatcgtga ccccttggtg cgattgcacc cggtgccacc agtccaccag ataggcctcg
     4921 tctaccggat cgtcgttctc gaagtagctg cggtcggcct tggggttgta gcgcttgccg
     4981 aaacctgaca gtcgggccgg gtcgagggcc tcgaggcgat aggcgagctc ggtgaagatc
     5041 tggaagtcgc tcttggactc gccgagcggg gcgatgacct tgggccgatg gatgtagtag
     5101 tgccccttgt accaaggcaa ggcgacatcg tggcgttcga agtgcgtggc caccggcagc
     5161 aggatgtcgg cccacagccc cgagggcgtg atcgtggagt cggcgcatac caccagctcg
     5221 agcttgcgga tggcttcgat ttccttgttg atgttggtca gctgattgaa ccagtccgag
     5281 ccatgccacc agatcgcctt gatgttcggg atgacgccgt cgaactggtc agcgcgcggc
     5341 cacaggccga tctcctcgcg ccgcacgtcg gggtagttga gtacgcaatg cgcccagcgg
     5401 tcggacttga tggaagccga ccacacgttg gcgtactcgt cataggggta ggccaccgct
     5461 tccgcgtgcc agcccttgcc gacgccttcc gcacagccgc cgaggatgcc gatgttgccg
     5521 gtcattgcct gcagggccgc cgccatgcgg ttgtactgct cgccgtaggc gttgcgcccg
     5581 ggtgaccacg aggccttcag cgccgccggc ttggtgcggg cgtacatgtc ggcaagcttg
     5641 cggatgtcgt ctgccgccac gccgcagatc tcggcagccc actccggcgt cttcggctgc
     5701 ccgtcgtagg tgccgaggat gtagtccttg aagttctcgt gcccgcgcgc ccagtccggc
     5761 atcgtgcccg cgtccacgcc caggcagaaa cggttgatga aggcctggtc ctgcagctca
     5821 ttgacaaaga tgtagtgcgc catcgcggcc agcatcgccg cgtcggtatt ggggcggatg
     5881 ggaatccacc aggcgtcata ggccgacgcc gaatccgtgt actgggggtc gaccacgacg
     5941 aacttgcagc cgcgctgctt ggccagccgc atgtagtgga acgtgttgcc gccgtggaag
     6001 gtgtaggccg ggttccagcc ccacatgatg atgagcttcg agtgcgcgaa ggcgtcgtcc
     6061 tcgttgccgt cctcgatggt gccgaacgtg gtgcggctgg agaaggttgt gccctggtag
     6121 cttggcacgg accaggagct ggttcggcaa ccgaacatcc cgaggaaacg tgccagcaag
     6181 ccctcgatct gatccgattt gtgcaggaca ccgtaggagg cgccggcata ggactggtcg
     6241 aggatggccg tcggtccgta ggtgtccttc agctcgatca tcttcttggc gatgcggtcg
     6301 agcgcttcgt cccacgacac gcgcttgaag cggccctccc cgcgttcgcc cacgcgcacc
     6361 atcgggtaga gcaggcgctc ggcggagtag agccgcgcgc ggtaagagcg tccgcgcaag
     6421 caggcgcgca gctgcggcac cgcctcggtc tcgaaaccaa aggcgccgcc tgcctggtag
     6481 cggccgtcat cggacgacag gcgaacgatc acgtctcctt tcttgtgcgc gaccagcatg
     6541 tggcgggagc cgcagttgtg cgcgcagctg gtcaccatgg tggtgagctg gtcgtcggtg
     6601 tagtagggct cgtaagcgaa ggccgaagcc tccttgcccg tgcttgccag ttccacgaag
     6661 ctgctggcgg cggccgcggc ggcgcccgcc gcggtggtct tgaggaagtc gcggcgcgcc
     6721 gggttgatcg gcgcggcgag tgtgtccttg ttcatggcga gttcccgtgc tgtcatttgg
     6781 ccgctggttt gtcgatcagg aagcgtgctt ccgaccgcgt cgggcgatcc ttggcccatt
     6841 cgggcacctc gtccgccatg ccgggccagg cgttacgtgc aaacggatag gcgatgcggt
     6901 accagggcct gccgccgtga cagccaaagc acacatcacc gccggccatg tgctcgcgtc
     6961 cggcctcggc tgcggcctcg atcgcggcgg gcttgaggta attgacctgg tgccgattgg
     7021 tacggaaggc cacgtgacag gcgaggcagt cgttctgcat gtcgatgcgc acctcgtgcc
     7081 aaggcccggg cagccccatg atcgtgtagt cggtgggctg gccatgacac ttcaggcaga
     7141 ttgcctcggt attgacggcc ttgcgcaggg tgaagtcgcc gcttccctgc gctgcggtgt
     7201 tcgccgacgt gcccggggcc tcgtcacgcg ggtcatgacc ctgatggcag gtcgtacatt
     7261 tcagatccat cagctgcttg gccagcggcg tgaccaggtg acgacggtgg aaactgtcct
     7321 gttcgccctg gtaggtggaa acctgttggt accacgctag gctgcccttg gccgcgacac
     7381 cggccggact ccgcgccagc ggcttgtcct cgaggacgtc ggcatggcag gcgaggcact
     7441 gtgcatcggt ggccgtctcg atcgccggct tgaagtgcag cgggctgtac gttgcgcgca
     7501 tgtgttcgcc aggccgctcg gcggagccgc cgggcttggc gggcttcttc gccgcggtct
     7561 tctttggtgc agcggcatgc cagccgcgca gatcgtaata gccgagcatt cccggttggt
     7621 cgaagggcgt gtaccaggcc tcctgatcgc gcatgacggc cagagtggcc ggtgcaggca
     7681 gcacgattcc gcgctgcaga cgggtctttg ccgcctcgct ggtcgtgtgc tggtggatca
     7741 ctggtgcgcc tgctggcctg gcatcaggtt gcaggcctgc aacgggataa gccggcgcgt
     7801 ccgccatcgc ggcgttgatg ccagcaagaa gaaggatcaa aacaggaaga cgcatctccg
     7861 gcactctcca ataagggagg aaggggggcg gcccccttcc cgtgcccagc tagggcaaac
     7921 agtcccgctt acttcttggg tgcgtcggcc gccatgcccg acatgccacc catgcccgac
     7981 atgccaccca tccccgacat gccccccatg cccgacatgc cgcccatacc cgacatgccg
     8041 cccataccgg acatgcccga catgccgccc atgccagcca taccggcggg gatgttgatg
     8101 attacggtcg tgccgcccat gccagacatg ccacccatgc cggccatgcc ggacataccg
     8161 cccataccgc ccatgccgga catgccaccc atgccggaca tgccgcccat gccacccata
     8221 ccggccatgc cgcccatgcc cgacatgccg cccatgccac ccatgcccga catgccgccc
     8281 atgccaccca tacccgacat gccacccatg ccacccatgc ccgacatgcc acccatgccc
     8341 tgctgctggg cgaagacgga gccggccatg gcaagggtca gcgcgctcgc ggcaacaacg
     8401 atcagttttt tcatggcgat acccatatca acgttgattg aatgcttgct
//
LOCUS       DAOQNN010000108         8447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4220_length_8447_cov_8.378575, whole genome shotgun sequence.
ACCESSION   DAOQNN010000108 DAOQNN010000000
VERSION     DAOQNN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4220_length_8447_cov_8.378575"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PKV97_07680"
     CDS             <1..732
                     /locus_tag="PKV97_07680"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306745.1"
                     /note="threonine deaminase; threonine dehydratase; in
                     Escherichia coli, IlvA is part of the isoleucine
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HNB05794.1"
                     /translation="AIKDVFEDTRSILEPAGALAVAGAKEYARRNKLRDKSLVAVASG
                     ANMNFDRLRFVAERAELGEQREAVLAVTIPEKPGSFRKFISVLGNRNITEFNYRYADA
                     ERAHIFVGVTVHNRSEVGRLVEMLERHELPALDLTDDEMAKSHVRYMVGGRAPQVENE
                     VVYRFVFPERPGALMNFLSNLRSDWNISLFHYRNHGSDFGRVLVGMQVPPADKDAFDA
                     FLQRLGYDYVAETDNPAYRMFLSGS"
     gene            complement(770..1345)
                     /locus_tag="PKV97_07685"
     CDS             complement(770..1345)
                     /locus_tag="PKV97_07685"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNB05795.1"
                     /translation="MSHDPGARRQRMREHALALRERLPAERRERLAAAIGEHLDDLVG
                     RLQPRRLAFCWPYRAEPDLRPWVQAWRAAAPGRVAALPVVLERHAPMMFRAWTPGVPM
                     PLDRHGIPHPADGEAVEPELVLIPLNAFDERGYRLGYGGGYFDRTLAVLRTVAVGVGF
                     EVGRVPDTLPQEFDRPMDWLVTEAGVAKPRA"
     gene            1406..3349
                     /locus_tag="PKV97_07690"
     CDS             1406..3349
                     /locus_tag="PKV97_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNB05796.1"
                     /translation="MKKALVAALTAATFLAPAGAFAQIGGESGDGRIVAAREALRKGD
                     RSTLERLAAVREAHPLDAYPRYWLLINRLARAEEPVPVAALQAFLAEEAGSNLAERLR
                     ADWLRRLARDGDWSGFLAVYADLRSPDAELRCNAWSARVLTGERSVFAELAREWDTLA
                     DADPTCDTPLRAAVDSGAVDEERVWWRIRRQIDTRKPEAALASLSLLPAGNAPAHADL
                     AQAIRSPAPWLDRLQPNFAVSRAGRELALAALVRLAREDVSAARLRLLRIQDRLGATE
                     RNYAHLALGMHAALDRLPEASALYAAAGDIETTAQQRAWRVRAALRIGDWHAVREAIE
                     AMPADEREAADWIYWLGRAHAAAGRRDAAESLYVRIAGEPHFYGMLAREELGEAFPPP
                     PATAPLPRAKLEEAERDPGLQRALALYRLELRTEALREWVWGVRERDEQFRLAAAHLA
                     LRNELYDRAINTAELANPRSNFELRFLTPYRDLIEPQVRAQGLDLGWVYGLMRQESRF
                     VVPARSSVGAQGLMQVMPATGKWVADRIGLAGYNQRLLTDPETNVLLGTSYMRLILEG
                     LDAHPVLASAGYNAGPGRARRWRDAAPLEGAIYTETIPFDETRDYVKKVLANAVIYAA
                     MLEKRPQSLKARLGTIAPGAASE"
     gene            3406..4362
                     /locus_tag="PKV97_07695"
     CDS             3406..4362
                     /locus_tag="PKV97_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HNB05797.1"
                     /translation="MNPKRVVIVGGSGFIGSAIANRLCKEGVKVLIPTRRRSRAGHVL
                     LLPNVEVVEGNVHDVALLARLFEGADAVVNTVGVLHSRPGRPFGPDFARAHVELPQKI
                     VAACRTAGVPRLVHISALGAYSDGPSEYQRSKAAGETAIRTGSPEIAWTILQPSVVFG
                     RGDSFLNLFADLARMFPVLPLAGATARFQPIYVEDVAEAVWQSLTRDDAGGQTFQLAG
                     PTVYTLRQLVEYVSALVGKPRPIYPLSEKLAMWQARLMELAPQPLMSRDNVRSMKVDN
                     VASGTPQPFGMVPTALETVAPAWLGQAAPRARYYPFRRHARR"
     gene            4388..5032
                     /locus_tag="PKV97_07700"
     CDS             4388..5032
                     /locus_tag="PKV97_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNB05798.1"
                     /translation="MKLIIGNKNYSSWSLRAWLAAREGGFTFEEIRIPLFIPGSRERI
                     LSHSPSGKVPCLNDHGVVVWDSLAIGEYLAEKRPGLLPADPVARALARSVTAEMHSGF
                     QNLRSRMPMNVRKDYTGFGHSPEVDADIARIVAIWNDCRARFGAVGPYLFGRFSLADA
                     AFAPVAFRFQTYAVKPAGAAGEYLATLLANPHMQEWATAARAETESIPEEDLYG"
     gene            5032..6258
                     /locus_tag="PKV97_07705"
     CDS             5032..6258
                     /locus_tag="PKV97_07705"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HNB05799.1"
                     /translation="MQAYVVGGAVRDALLGLPVKDRDWVVVGSTPEEMLARGFRPVGR
                     DFPVFLHPHTNEEYALARTERKSGHGYTGFVCHASPEVTLEEDLLRRDLTINAIARAE
                     DGRLIDPCGGRRDLAARVFRHVSPAFAEDPLRILRVARFSARFTDFELAPETLELMRA
                     MVVAGEVDHLVAERVWQELARGLMEARPSRMIRVLRACGALARLLPELDRLFGIPQPE
                     KYHPEIDTGEHVLMVLDHAAAADEPLAVRWACLLHDLGKGDTPAHLLPHHYGHEAASA
                     RRAREVSERLKAPADCRDLAEMVAREHGILARAHELRAETMVKLIERCDGLRRPQRFL
                     LMLQAAACDHAGRGSGAENASEWRPLSRWQAALQAVRAVDAGRIATECSDKSLIPQAL
                     HTARVAAVKALDKDPA"
     gene            6255..7058
                     /locus_tag="PKV97_07710"
     CDS             6255..7058
                     /locus_tag="PKV97_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB05800.1"
                     /translation="MNATRFGELEVLCHAPERATHEHPLLFIHGAYVSAWCWDEHFLP
                     WFARRGWQAYAVSLSGHGRSRQREHLDSYSIDDYVRDVAEVAARLPAPPVLVGHSMGG
                     MVVQKYLEQHDAPAAVLMSAVPPQGLMGSAFGLIFKRPHLLSDLNRIMSGNDVDIDSL
                     REALFHQPVAADDLQRYYRLSQPESHRAIWDMSLFNLPQPARMHRVPMQILGAEHDAL
                     IPPDQVHMTAMTYGRQAEILPGMGHGMMLEHDWEIAAMRIADWLAERAL"
     gene            complement(7075..8250)
                     /locus_tag="PKV97_07715"
     CDS             complement(7075..8250)
                     /locus_tag="PKV97_07715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB05801.1"
                     /translation="MSSLPQPSADALAQSARLLEHIEAELAAAAGWIPFARYMELALY
                     APGLGYYSGGARKFGPGGDFITAPELTPLFGQALAAQVEQVMRASAPALIEVGAGTGL
                     LAADLLLELERRGCLPERYGILELSGELRERQFDTLAAKVPHLAARVQWLDALPERFS
                     GAVVANEVLDVMPVHLLVSRAEGLFERGVAIATDAAGIRRLCWADVPAAGAVAEGARA
                     LALPVPQSGEYVTELNLAGKAWVAAWAERLHAGALLLIDYGYPRAEYYLPSRSGGTLL
                     CYYRHHAHGDPFLWPGLNDITAFVDFTAVAEAGFEAGLDVQGYTTQAQFLFNCGVLEC
                     LERRGARESADYIRAARAVQRLTAPQEMGELFKVIALSRAIDGPLLGFARGDRTHAL"
BASE COUNT         1256 a         3226 c         2818 g         1147 t
ORIGIN      
        1 gcgatcaagg acgtcttcga ggacacccgc tccatcctcg agcccgccgg cgcgctcgcc
       61 gtcgccggcg ccaaggagta cgcccgccgc aacaagctgc gcgacaagag cctggtggcg
      121 gtggcctcgg gggccaacat gaacttcgac cgcctgcgct tcgtcgccga gcgcgccgag
      181 ctcggcgagc agcgcgaggc cgtgctcgcg gtgaccatcc ccgagaagcc gggctccttc
      241 cgcaagttca tcagcgtgct cggcaaccgc aacatcaccg agttcaacta ccgctacgcc
      301 gacgccgagc gcgcccacat cttcgtcggc gtcaccgtgc acaaccgcag cgaggtcgga
      361 cgcctggtgg agatgctcga gcgccacgag ctgcctgcgc tcgacctcac cgacgacgag
      421 atggccaagt cgcacgtgcg ctacatggtg ggcggacgcg cgccacaggt ggaaaacgag
      481 gtggtatacc gcttcgtctt ccccgagcgc ccgggcgcgc tgatgaattt cctctccaac
      541 ctgcgctcgg actggaacat ctcgctgttc cactaccgca accacggctc cgacttcggc
      601 cgcgtgctgg tcggcatgca ggtgccgccg gccgacaagg acgccttcga cgccttcctg
      661 cagcggctcg gctacgacta cgtggccgag accgacaacc ccgcctaccg catgttcctg
      721 tccgggagct gagccccgca ggcaggccgt gcagggttgc agccgccgct caggcgcgcg
      781 gcttcgccac ccccgcctcg gtcaccagcc agtccatcgg gcgatcgaac tcctgcggca
      841 gcgtgtcggg cacgcgcccg acctcgaagc ccacgcccac cgccacggtg cgcagcaccg
      901 cgagcgtgcg gtcgaagtag ccccctccgt agccgaggcg gtagccgcgt tcgtcgaagg
      961 cattgagggg gatcagcacc agctcgggct cgaccgcctc gccgtccgcc gggtggggga
     1021 tgccatggcg gtcgagcggc atcggcacgc ccggcgtcca cgcgcggaac atcatcggcg
     1081 catggcgctc gagcaccacc ggcaaggccg ccacccgccc gggcgcggcc gcacgccagg
     1141 cctgcaccca cgggcgcagg tcgggctcgg cgcggtaggg ccagcagaag gcgagccggc
     1201 gtggctgcaa gcgtccgacc aggtcgtcga gatgctcgcc gattgccgcg gcgaggcgct
     1261 cgcgacgctc ggcaggcagg cgctcgcgca acgccagcgc atgttcgcgc atgcgctggc
     1321 gacgcgcgcc gggatcatgg gacaaggggg cgaaactcat cttcgatatg gtatggttgg
     1381 cggtcaaacg gaggcatcac ctggcatgaa gaaggcactg gttgcggcgc tcacggccgc
     1441 gacgttcctc gcccccgccg gcgcgttcgc gcagattggc ggcgagtccg gggacgggcg
     1501 tatcgtcgcc gctcgcgagg ccctgcgcaa gggcgaccgc agcacgctcg aacgcctcgc
     1561 agccgtgcgc gaggcccacc ccctcgacgc ctatccgcgc tactggctgc tgatcaaccg
     1621 cctcgcgcgt gccgaagaac ccgtgcccgt cgcggccctg caagccttcc tcgccgaaga
     1681 agccggctcc aacctcgccg aacgcctgcg cgccgactgg ctgcgccgcc tcgccaggga
     1741 cggcgactgg agcggcttcc tcgcggtgta cgcggacctg cgcagccccg acgccgaatt
     1801 gcgctgcaac gcctggagcg cgcgcgtgct gaccggcgag cgcagcgtct tcgccgagct
     1861 cgcgcgcgaa tgggacaccc tcgccgacgc cgatccgacc tgcgacacgc cgctgcgcgc
     1921 ggcggtcgac tccggcgcgg tggacgaaga acgcgtgtgg tggcgcatcc gccgacagat
     1981 cgacacccgc aagcccgagg ctgcgctcgc cagcctgtcg ctgctgcccg ccggcaacgc
     2041 cccggcccac gccgacctgg cgcaggcgat ccgctcgccc gcaccctggc tggatcggct
     2101 gcagcccaac ttcgccgtga gccgcgccgg ccgcgagctc gcgctcgccg cactggtgcg
     2161 cctggcgcgc gaggacgtct ccgccgcgcg cctgcgcctg ctgcgcatcc aggaccgcct
     2221 cggcgcgacc gagcgcaact acgcccatct ggcgctcggc atgcacgccg cgctcgaccg
     2281 cctgcccgag gcgagcgcgc tctacgccgc cgccggcgac atcgagacca ccgcccagca
     2341 acgtgcgtgg cgggtgcgtg cggcgctgcg catcggcgac tggcacgcgg tgcgcgaggc
     2401 gatcgaagcc atgcccgccg acgaacgcga agctgccgac tggatctact ggctgggccg
     2461 cgcacacgcc gcggccgggc gccgcgacgc cgccgagtcg ctctacgtgc gtatcgccgg
     2521 cgagccgcac ttctacggca tgctcgcgcg cgaggagctc ggcgaggcct tcccgccgcc
     2581 ccccgccacc gcgccgctac cgcgcgccaa gctcgaggag gccgagcgcg accccggcct
     2641 gcagcgcgca ctcgcgctct accggctgga gctgcgcacc gaggcgctgc gcgaatgggt
     2701 ctggggcgtg cgcgaacgcg acgagcagtt tcgcctcgcc gccgcccacc ttgcgctgcg
     2761 caacgagctc tacgaccgcg cgatcaatac cgccgagctg gccaacccgc gcagcaactt
     2821 cgagctgcgc ttcctgaccc cctatcgcga cctcatcgag ccgcaggtgc gcgcgcaggg
     2881 gctcgacctc ggctgggtgt acggcctcat gcgccaggag agccgcttcg tggtcccggc
     2941 acgctccagc gtcggcgccc agggcctcat gcaggtgatg cccgccaccg gcaagtgggt
     3001 ggcggatcgg atcggcctcg ccggctacaa ccagcgcctg ctcaccgacc ccgagaccaa
     3061 cgtgcttctg ggcaccagct acatgcgtct catcctggag gggctcgacg cccaccccgt
     3121 gctcgccagc gccggctaca acgccggccc cggccgggcg cggcgctggc gcgacgcggc
     3181 gccgctcgag ggcgcgatct ataccgagac gatcccgttc gacgagaccc gcgactacgt
     3241 caagaaggtg ctcgccaacg cggtgatcta cgcggcgatg ctcgaaaaac ggccgcaatc
     3301 gctcaaggcg cgactcggca cgatcgcgcc cggggctgcc agcgagtaag cttccgcgtg
     3361 cgccactcca ttccatccac agcaacagca cggtccagtc gaaacatgaa cccgaaacgc
     3421 gtcgtcatcg tcggcggctc cggctttatc ggcagcgcca tcgccaatcg tctctgcaag
     3481 gaaggcgtca aggtcctcat tcccacccgc cgccgcagcc gcgccggcca tgttctgctg
     3541 ctacccaacg tcgaggtggt ggagggcaac gtgcatgacg tcgccctgct tgcgcgcctg
     3601 ttcgagggcg ccgacgccgt ggtgaacacc gtcggcgtcc tgcattcgcg cccgggcagg
     3661 cccttcggcc ccgacttcgc gcgcgcccac gtcgaactgc cgcagaagat cgtcgctgcc
     3721 tgtcgcacgg cgggcgtgcc ccgcctcgtc cacatcagcg ccctcggcgc ctattccgac
     3781 ggcccgtcgg aataccagcg ctcgaaggcc gccggcgaga ccgcgatccg caccggctcg
     3841 cccgagatcg cgtggaccat cctgcagcct tcggtggtgt tcggccgcgg cgacagcttc
     3901 ctcaacctct tcgccgacct cgcgcgcatg ttcccggtgc tgccgctcgc cggcgccacc
     3961 gcgcgcttcc agccgatcta tgtcgaggac gtcgccgagg cggtgtggca gagcctgacg
     4021 cgcgacgatg caggcgggca gaccttccag ctcgccggac cgaccgtcta cacgctgcgc
     4081 cagctcgtcg agtacgtctc cgcgctcgtg ggcaagccgc gcccgatcta tccgctgtcc
     4141 gagaagctgg cgatgtggca ggcgcgcctc atggagctcg ccccccagcc cctgatgagc
     4201 cgcgacaacg tgcgctcgat gaaggtcgac aacgtcgcca gcggcacccc gcaacccttc
     4261 ggcatggtgc ctaccgcgct cgagaccgtg gccccggcct ggctcggcca ggcggcgccg
     4321 cgagcccgtt actacccctt ccgccgccac gcgcggcgct gaagagcaac tctctacgga
     4381 gtccgcgatg aagctgatca tcggcaacaa gaactattcc tcctggtccc tgcgcgcctg
     4441 gctggcggcg cgcgagggtg gcttcacctt cgaggagatc cgcatcccgc tgttcatccc
     4501 cggcagccgc gagcgcatcc tgtcgcactc gccctcgggc aaggtgccct gcctgaacga
     4561 ccacggcgtg gtggtgtggg actcgctcgc gatcggcgaa tacctcgccg agaagcgccc
     4621 cggtctgctg cccgccgacc cggtcgcacg cgcgctggcg cgctcggtca ccgccgagat
     4681 gcactccggc ttccagaacc tgcgcagccg catgccgatg aacgtgcgca aggactacac
     4741 cggtttcggc cacagccccg aggtcgatgc cgacatcgcg cgcatcgtgg cgatctggaa
     4801 cgactgccgc gcgcgcttcg gcgccgtggg cccctacctc ttcggccgct tcagcctcgc
     4861 cgacgcggcc ttcgcgccgg tggccttccg cttccagacc tacgcggtga agcccgcggg
     4921 cgcggccggc gagtacctcg ccaccctgct cgccaacccg cacatgcagg agtgggcgac
     4981 ggcggcacgc gccgagaccg agtcgatccc ggaagaggac ctctacggct gatgcaggcc
     5041 tatgtcgtgg gcggagcggt gcgcgacgcc ctgctcggcc tgccggtgaa ggatcgcgac
     5101 tgggtggtgg tcggcagcac gcccgaggag atgctcgcgc gcggcttccg cccggtcggc
     5161 cgcgattttc cggtcttcct gcatccgcac accaacgagg aatacgcgct cgcgcgcacc
     5221 gagcgcaagt cgggccacgg ctacaccggc ttcgtctgcc acgcctcgcc cgaggtcacg
     5281 ctcgaagagg acctgctgcg ccgcgacctc accatcaacg cgatcgcgcg cgccgaggac
     5341 ggccgcctga tcgacccctg cggtggccgg cgcgacctcg ccgcgcgcgt cttccgccat
     5401 gtgtcgcccg ccttcgccga ggatccgctg cgcatcctgc gcgtggcgcg cttctccgcg
     5461 cgcttcaccg acttcgagct ggcacccgag accctggaac tgatgcgcgc gatggtcgtg
     5521 gccggcgagg tcgaccatct ggtcgccgag cgcgtctggc aggaactggc gcgcgggctc
     5581 atggaggcgc gcccctcgcg catgatccgc gtgctgcgcg cctgcggcgc actcgcacgc
     5641 ctgctgcccg agctcgaccg cctgttcggg atcccgcagc cggagaagta ccaccccgag
     5701 atcgacaccg gcgagcacgt gctgatggtg ctcgaccacg ccgccgccgc ggacgagccg
     5761 ctcgcggtgc gctgggcctg cctgctgcac gacctcggca agggcgacac gcccgcccac
     5821 ctgctgccgc accattacgg ccacgaggcc gcgagcgcgc gccgcgcgcg cgaggtgtcg
     5881 gaacgcctga aggcccccgc cgactgccgc gacctcgccg agatggtggc gcgcgagcac
     5941 ggcatcctgg cgcgcgcgca cgagctgcgc gccgaaacca tggtcaagct gatcgaacgc
     6001 tgcgacggcc tgcgccgtcc gcagcgcttc ctcctgatgc tgcaggcggc cgcctgcgac
     6061 cacgccggcc gcggcagcgg cgcggaaaac gcgtcggaat ggagaccctt gtcacgctgg
     6121 caggccgcgc tgcaggccgt gcgcgccgtc gatgccggta gaatcgcaac cgagtgcagc
     6181 gacaaatcgc tgattcctca ggccttgcac accgcccgcg tggcggccgt gaaggccctg
     6241 gacaaggacc ccgcatgaac gcaacccgct tcggcgaact cgaggtgctg tgtcacgcac
     6301 ccgagcgcgc cacccatgaa caccccctgc tcttcatcca cggcgcctac gtttcggcat
     6361 ggtgctggga cgagcacttc ctgccctggt tcgcccgccg cggctggcag gcctatgccg
     6421 tctcgctctc cggccatggc cgcagccgcc agcgcgagca cctcgactcc tactcgatcg
     6481 acgactacgt gcgcgacgtc gccgaagtcg ccgcccgcct gcctgcgccg ccggtgctgg
     6541 tcggccactc gatgggcggg atggtggtgc agaagtatct cgagcaacac gacgcgcccg
     6601 ccgcggtgct gatgtccgcc gtcccgccgc aaggcctgat gggctcggcc ttcggcctga
     6661 tcttcaagcg cccgcacctg ctctccgacc tcaaccgcat catgtcgggc aacgacgtgg
     6721 acatcgacag cctgcgcgag gcgctcttcc atcagccggt ggcggccgac gacctgcaac
     6781 gctactaccg cctgagccag cccgagtcgc accgcgcgat ctgggacatg tcgctgttca
     6841 acctgccgca gccggcgcgc atgcaccgcg tgccgatgca gatcctcggc gccgagcacg
     6901 acgccctgat cccgcccgac caggtgcaca tgaccgccat gacctacggt cgccaggccg
     6961 agatcctccc cggcatgggc cacggcatga tgctcgagca cgactgggag atcgccgcga
     7021 tgcggatcgc cgactggctg gccgagcgcg cgctgtagcc gacgccgggc cgcctcagag
     7081 cgcgtgcgtg cgatcgccgc gcgcgaagcc gagcagcggt ccgtcgatcg cgcgcgacag
     7141 cgcgatcacc ttgaagagct cccccatctc ctgcggcgcg gtcaggcgct gcaccgcgcg
     7201 cgcggcgcgg atgtagtccg cgctctcgcg ggcgccgcgc cgctccaggc attccagcac
     7261 gccgcagttg aagaggaact gcgcctgcgt ggtgtagccc tgcacgtcca gcccggcctc
     7321 gaagccggcc tcggccaccg cggtgaagtc cacgaaggcg gtgatgtcgt tgagccccgg
     7381 ccacaggaaa gggtcgccgt gggcatggtg gcggtagtag cacagcaggg tgccgcccga
     7441 acgcgagggc aggtagtact cggcgcgcgg atagccgtag tcgatcagca gcagggcgcc
     7501 cgcgtgcagg cgctcggccc aggccgccac ccaggccttg ccggcgaggt tcagctcggt
     7561 gacgtattcc ccgctctgcg gcaccggcag ggcgagcgcc cgcgctcctt ccgccaccgc
     7621 gcccgccgcc ggcacgtccg cccagcacag ccggcgtatc cccgcggcat cggtggcgat
     7681 ggcgacgccg cgctcgaaga gcccctcggc gcgcgacacc agcagatgca ccggcatcac
     7741 gtcgagcacc tcgttggcca ccactgcacc ggagaagcgc tccggcagcg cgtccagcca
     7801 ctgcacgcgc gccgccaggt gaggaacctt ggcggccagg gtgtcgaact ggcgttcgcg
     7861 caattcgccc gagagctcga ggatgccgta gcgctcgggc aggcagccgc ggcgttcgag
     7921 ctcgagcagc aggtcggcgg cgagcaggcc ggtaccggcg ccgacctcga tcagcgcggg
     7981 cgcgctcgcg cgcatcacct gctcgacctg ggcggcgagc gcctggccga aaagcggggt
     8041 aagctcgggc gcggtgatga aatcgccgcc ggggccgaac tttcgcgcgc caccgctgta
     8101 ataccccagc cccggcgcgt agagcgcgag ctccatgtag cgcgcgaacg ggatccagcc
     8161 agcggccgcg gccagctcgg cctcgatgtg ttcgagcagg cgggcgctct gggcgagcgc
     8221 atcggcggaa ggctggggca atgaagacat gatcgttctg caggacagga gggcgaggcc
     8281 gcgattgtag cggcacgccc gcccagccgc ccggccagct ccgggcctca cggtgaatcc
     8341 gatgcgcacg tctcctcccg aacccgaatc caccgcggca ccgctgatcc tggtcaccgg
     8401 cgccgcgcgc cgcgtcggcg ctgcgatcgc acgccggctg cacgccg
//
LOCUS       DAOQNN010000109         8388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4274_length_8388_cov_9.473179, whole genome shotgun sequence.
ACCESSION   DAOQNN010000109 DAOQNN010000000
VERSION     DAOQNN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4274_length_8388_cov_9.473179"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="PKV97_07720"
     CDS             complement(<1..1339)
                     /locus_tag="PKV97_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB05802.1"
                     /translation="MSAVNEFLAARDFLLQHRSDYATAYAGFKWPKLKEFNWALDYFD
                     AMAKGNDNPALWIIEEDGRESKLSFAEMSARSNRVANWLRKQGVKRGERILIMLGNEV
                     PLWETMLAAIKLGAVVIPATTLLTPEDLVDRVERGQVRHVVIGKAHTDKFENLPGSFG
                     RIAVGGAPAGWKAFEDAAAESDAFTPDGVTRVDDPLLLYFTSGTTSKPKLVLHTHQSY
                     PVGHLSTMYWIGLKPNDRHMNISSPGWAKHAWSCFFAPWNAGACVFLYNYNRFNAAAL
                     LDVLVKYEVTTMCAPPTVWRMLIQQDLASVKTKLRELIGAGEPLNPEVIDQVKKAWGI
                     TIRDGFGQTETTAQIGNTPDQPLKPGSMGRPLPGYKIALLDAESKPSVEGEVALVMGE
                     HRPVGLMVGYSGDPAKTAEVMRDGHYRTGDVASIDEDGYITYVGRADDVFKASD"
     gene            1599..2420
                     /locus_tag="PKV97_07725"
     CDS             1599..2420
                     /locus_tag="PKV97_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HNB05803.1"
                     /translation="MPPPSSRLQARYGQESIPAEIALNPLVEHLLDHRSVRAYLPDPV
                     TDGELAAIVAAAQSAASSSNLNVWSVVAVRDADRRARLAELAGDQAHVREAPLQLVWL
                     ADLARLRRIATALERPAEGLDYLEMFLTGVIDAALAAQNGAAAAESLGLGMVYIGGMR
                     NHPEEVAALLGLPPGVFAVFGMCVGRPDPARPADIKPRPPQAVVLHHERYSLDAQDAG
                     IAAYDRAMTRFYAEQKMNVHGTWSVHSSKRIAGPETLSGRHRLVEALQALGFALK"
     gene            2455..3870
                     /locus_tag="PKV97_07730"
     CDS             2455..3870
                     /locus_tag="PKV97_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB05804.1"
                     /translation="MRKRDTTLSTDQKALAINLDKYKYGSIVEIGAGQEVARRFFHVG
                     AAAGTIAKTMSAYDMAVSDDIYGHVDRYVSRARLLQMLDKEFTQVVARLAHVRPKNTT
                     FFAYAATVTARSFKQKNECHGWVGIRLQLHPGAAPSDVVMHVRMLDNDNALQSEALGI
                     LGVNLIHGAFFHHDQPEWVVEGLADGLGSERIEVDLIHFSGPYFDEVENRLMNLHLIR
                     SWLTRAVVFNPQGEVVVPGELLYRKPVMVMRGSFKPVTLVNVDMMAAGLRQFSQLAAV
                     DDKEIVSLAEITMNSLISGDNVDGADFLARIDLLASQGYTVMISDYVRFFRLRAYLRR
                     YTQKPIGIVLSVRDFAFLFDEKYYEGLEGGILEAFGKLFPDNTHVYVYPSRPRGTDAS
                     ATVTLDNVAVPANLRHLLAHLKENDKLVAVQPHDDTHLHIDIGEVLTGLRRGRGEWET
                     DVPEAVAQRIIEGRLLGYDTE"
     gene            4062..6776
                     /locus_tag="PKV97_07735"
     CDS             4062..6776
                     /locus_tag="PKV97_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNB05805.1"
                     /translation="MSPRTAQLSPIENAHARAADEVLAALDSSPTGLSADEAARRLAE
                     IGPNRLPEPPRDGVVKRFFKHFNDVLIYVLLGAAAITASLGHWIDTGVILGVVVINAI
                     IGFIQEGKAEEALAGIRKMLSLRAEACRDGHWSAVDADGLVPGDIVRLRSGDRVPADV
                     RLIEATNLRIEESALTGESVPADKNTAAVEAAAGVGDRFGMAYSGTLVAAGRGIGVAV
                     ATGPDTELGRIDRLIAEVQPLQTPLTRQMAQFSKILSVLIVGLALLMLGVGLAVHEQP
                     LAEVFLAAIGFAVAAIPEGLPAILTITLALGVQRMARRNAITRKLTAVEALGSVTVIC
                     SDKTGTLTKNEMTVRHVLTRGGFYEVSGTGYRPEGEITLEGKVASLAHHDHLHALIEV
                     VAVANDTHIAEEDGQWKVTGEPTEGALRTLAHKAGFDAGAHQRHDSIPFESANKFMAT
                     LDTAPCGQRRILLKGAPDRLLERCRHELGADGELVALDRAFWEERIDALSGEGLRVLA
                     AAARDVPDSKDALVIEDLQEDMVFLGLIGIIDPPRPEAIAAIRSCHEAGIRVKMITGD
                     HAGTARAIGIEMGLGTADALKVITGAEIEAASDEELQRIAHDCDLFARTSPEHKLRLV
                     EALQARGEVVAMTGDGVNDAPALKRADVGVAMGIKGTEATKEAAEIVLADDNFSTIER
                     AVEEGRTIYDNLRKAILFILPTNGAQGFVMLAAVVLGLTLPLTPVQILWVNMVVAVTL
                     ALALAFEPAEPGVMQRPPRRPGAPIMDGAMLWRVGFVSLLIGGATIVSFEYSLLQGME
                     LQAARTMAVNTLVVAQAWYLFNSRFLTAPSLSPARLFTNPAAQVSVLVLALLQLGFVY
                     LPFMNTWFGTTPLALEYWLPPLAIGALVFLVIEAEKALQRRYGARKSG"
     gene            7054..7944
                     /locus_tag="PKV97_07740"
     CDS             7054..7944
                     /locus_tag="PKV97_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNB05806.1"
                     /translation="MSKHKPIDIAEFRVEGGKKVDLADWPTKVRAVYDSKSDYEERLD
                     AGTERLAELQEMLYAHDRYSLLVIFQAMDAAGKDGAIRHVMSGINPQGCQVFSFKHPS
                     PTELDHDFLWRTHVALPERGRIGIFNRSYYEEVLIVRVLPEILRGQQLPDETLAARDF
                     WGERFRSIVDAEAHLHRNGTRIVKIFLHLSKDEQRDRLIARIDEEDKNWKFDEGDIEQ
                     RKHWDKYMAAYADCLRATSTPDCPWYVVPADDKKNARLIVSQILVDTLAGLDLHYPET
                     SPARREKLLEIRRQLEAGEV"
BASE COUNT         1333 a         3074 c         2719 g         1262 t
ORIGIN      
        1 agtcggaggc cttgaacacg tcgtcggcgc ggccgacgta ggtgatgtag ccgtcctcgt
       61 cgatgctggc gacgtcgccg gtgcggtagt ggccgtcacg catgacctcg gcggtcttgg
      121 ccggatcacc cgagtaaccc accatcaggc cgaccggacg gtgctcgccc atcaccagcg
      181 ccacctcgcc ctcgaccgag ggcttgctct cggcatcgag cagggcgatc ttgtacccgg
      241 ggagcggacg gcccatggag ccgggcttga gcggctggtc gggggtgttg ccgatctgcg
      301 cggtggtctc ggtctggccg aagccgtcgc ggatggtgat gccccaggcc ttcttgacct
      361 ggtcgatgac ctcggggttg agcggctcgc cggcaccgat gagctcgcgc agcttggtct
      421 tgaccgaggc caggtcctgc tggatcagca tgcgccacac ggtcgggggc gcgcacatcg
      481 tggtgacttc gtacttcacc agcacgtcga gcagggccgc cgcgttgaag cggttgtagt
      541 tgtacaggaa cacgcaggcg ccggcgttcc agggcgcgaa gaagcagctc caggcgtgct
      601 tggcccagcc cggcgaggag atgttcatgt gacgatcgtt gggcttgagg ccgatccagt
      661 acatcgtcga caggtggccg accgggtagg actggtgggt gtgcagcacc agcttgggct
      721 tggaggtcgt gcccgacgtg aagtagagca gcagcgggtc gtcgacgcgg gtcacgccgt
      781 ccggggtgaa ggcgtcggat tcggccgcgg cgtcctcgaa ggccttccag ccggcaggcg
      841 cgccgccgac ggcgatgcgg ccgaagctgc cgggcaggtt ctcgaacttg tcggtgtgcg
      901 ccttgccgat caccacgtgc ctgacctggc cgcgctcgac gcggtcgacg aggtcctcgg
      961 gggtcagcag cgtggtggcg gggatcacca cggcgccgag cttgatcgcg gcgagcatgg
     1021 tctcccacag cggcacttcg ttgccgagca tgatcaggat gcgctcgccg cgcttgacgc
     1081 cctgcttgcg cagccagttg gcgacgcggt tcgagcgcgc cgacatctcg gcgaaggaaa
     1141 gcttcgactc gcggccgtct tcctcgatga tccacagcgc cgggttgtcg ttgcccttgg
     1201 ccatcgcatc gaagtagtcc agcgcccagt tgaactcctt cagcttgggc cacttgaagc
     1261 cggcgtaggc cgtcgcgtaa tcgctgcggt gctgcagcag gaaatcgcgc gcggcgagaa
     1321 attcgttgac tgcgctcatg gttctctcct ctccaggacg tcgttggaat gctccggacg
     1381 ggtgcatgcc cgcccgatcc cgctatttta cgttgacgtt aacgcagcgc accattcttg
     1441 atcgaaatta tgtttatgac cggcaaagtg cttcacccgc ccgatcgccg cggcaagcgc
     1501 cctcgtcgct cgcacccggg ctgcggggcg ccgcatccac tgtcgatata cttgccgctt
     1561 gcgagcgagt atcgccgccg atcgtgcaag gagcaaccat gccccccccc tcttcccgcc
     1621 tgcaagcccg ctacggccag gaatccatcc ccgccgagat cgccctcaac ccgctcgtcg
     1681 agcacctgct cgaccaccgc tcggtgcgcg cctacctgcc cgacccggtc accgatggcg
     1741 agctcgccgc gatcgtcgcc gccgcgcaat cggcggcgag ttcgtccaac ctcaacgtgt
     1801 ggagcgtggt cgcggtgcgc gacgccgatc gccgcgccag gctggccgag cttgccggcg
     1861 accaggccca cgtgcgcgag gcgccgctgc agctcgtgtg gctcgccgac ctcgcccgcc
     1921 tgcgccgcat cgccaccgcc ctggagcgcc cggccgaggg cctcgactac ctcgagatgt
     1981 tcctcaccgg cgtcatcgac gccgcgctcg ccgcgcagaa cggcgcggcg gcggccgaat
     2041 cgctcggcct cggcatggtg tacatcggcg gcatgcgcaa ccaccccgaa gaggtcgccg
     2101 cgctgctcgg cctgccgccc ggcgtgttcg cggtgttcgg catgtgcgtc ggccgtcccg
     2161 atccggcgcg cccggccgac atcaagccgc gcccgccgca agcggtggtg ctgcaccacg
     2221 aacgctactc gctcgacgcc caggacgccg gcatcgccgc ctacgaccgc gcgatgacgc
     2281 gcttctacgc ggagcagaag atgaacgtgc atggcacctg gagcgtgcac tcgtccaagc
     2341 gcatcgccgg ccccgagacg ctctccggcc gccaccgcct ggtcgaggcg ctccaggcgc
     2401 tcggcttcgc gctcaagtaa tcccctccct cctgcccgca aagaaggatc cgccatgcgc
     2461 aaacgcgaca cgacgctgag caccgaccag aaggcgctcg ccatcaacct ggacaagtac
     2521 aaatacggct ccatcgtcga gatcggcgca gggcaggagg tggcgcgccg cttcttccac
     2581 gtcggcgccg ccgccggcac catcgccaag accatgtcgg cctacgacat ggcggtcagc
     2641 gacgacatct acggccacgt cgaccgctac gtcagccgtg cccgcctgct gcagatgctc
     2701 gacaaggagt tcacccaggt cgtcgcccgc ctcgcccacg tgcggccgaa gaacaccacc
     2761 ttcttcgcct acgccgccac cgtcaccgcg cgcagcttca agcagaagaa cgaatgccac
     2821 ggctgggtgg gcatccgcct gcagctgcac ccgggcgccg cgccgagcga cgtggtcatg
     2881 cacgtgcgca tgctcgacaa cgacaacgcg ctgcagtccg aggcgctcgg catcctcggc
     2941 gtcaacctca tccacggcgc cttcttccac cacgaccagc ccgagtgggt ggtggaaggt
     3001 ctcgccgacg ggctcggctc cgagcgcatc gaggtcgacc tgatccactt ctccggcccg
     3061 tatttcgacg aagtggaaaa ccgcctgatg aacctgcacc tgatccgcag ctggctcacg
     3121 cgcgcggtgg tcttcaaccc gcagggcgag gtggtggtgc cgggcgagct cctctaccgc
     3181 aagccggtga tggtgatgcg cggcagcttc aagccggtga ccctggtgaa tgtggacatg
     3241 atggccgccg gcctgcgcca gttcagccag ctcgccgcgg tcgacgacaa ggagatcgtc
     3301 tcgctcgccg agatcaccat gaactcgctg atcagcggcg acaacgtgga cggcgccgac
     3361 ttcctcgccc gcatcgacct gctcgcctcg cagggctaca cggtgatgat ctcggactac
     3421 gtgcgcttct tccgcctgcg cgcctacctg cgccgctaca cccagaagcc gatcgggatc
     3481 gtgctgtcgg tgcgcgactt cgccttcctg ttcgacgaga aatactacga aggcctggag
     3541 ggcggcatcc tcgaggcctt cggcaagctt ttcccggaca acacccacgt ctatgtgtat
     3601 ccgtcccgcc cgcgcggcac cgacgcctcg gccacggtga cgctcgacaa cgtggccgtc
     3661 cccgccaacc tgcgccacct gctcgcccac ctcaaggaaa acgacaagct ggtggcggtg
     3721 cagccgcacg acgacaccca cctgcacatc gacatcggcg aggtgctcac cggcctgcgc
     3781 cgcggccgcg gcgagtggga gaccgacgtg cccgaggcgg ttgcgcaacg catcatcgaa
     3841 ggccgcctgc tcggctacga taccgagtga gcggccctgt gcacgccccc acgactccgg
     3901 accctgcagc acgcggggtc tttttttgaa cttcggcggg agcgacggtc tgtattaatg
     3961 cagcgcaaca tccgcgccgc aggcttgcac aagcctcttg ctttcgatcg cgcgcgcggc
     4021 atcctgcgca cagcgcatcc gcaccggcac ggagcacgga catgagcccg cgaaccgccc
     4081 agctttcccc catcgagaac gcccacgccc gcgcggccga cgaggtcctc gcagcgctgg
     4141 acagcagccc gacgggcctc tctgcggacg aggccgcacg acgtctcgcc gagatcggcc
     4201 ccaaccgcct gcccgagccg ccccgggacg gcgtggtcaa gcgcttcttc aagcatttca
     4261 acgacgtgct gatctacgtg ctgctcggcg ccgccgcgat cacggcaagc ctcggccact
     4321 ggatcgacac cggcgtcatc ctcggcgtgg tggtcatcaa cgccatcatc ggcttcattc
     4381 aggaaggcaa ggccgaggag gcgcttgcag gcatccgcaa gatgctgtcc ctgcgtgccg
     4441 aggcctgccg cgacggacac tggagcgcgg tggacgccga cggcctggtg cccggcgaca
     4501 tcgtgcgcct gcgctcgggc gaccgcgtgc ccgccgacgt gcgcctgatc gaggccacca
     4561 acctgcgcat cgaggagtcc gcactcaccg gcgagtcggt tcctgccgac aagaacaccg
     4621 ccgcggtcga ggccgccgcc ggcgtcggcg accgcttcgg catggcctat tcaggcaccc
     4681 tggtcgccgc cggacggggc atcggcgtgg ccgtcgccac cgggcccgac accgagctcg
     4741 gccgcatcga ccgcctgatc gccgaggtgc agcccctgca gaccccgctc acccggcaga
     4801 tggcgcagtt cagcaagatc ctgtcggtgc tcatcgtcgg cctggcgctg ctcatgctgg
     4861 gcgtcggcct cgcggtgcac gagcagccgc tggccgaggt cttcctcgcc gcgatcggct
     4921 tcgcggtcgc ggccatcccc gaaggcctgc ccgccatcct caccatcacg ctcgcgctcg
     4981 gcgttcagcg catggcgcgg cgcaacgcga tcacgcgcaa gctcaccgcg gtcgaggcgc
     5041 tcggctcggt cacggtgatc tgctcggaca agaccggcac gctgacgaag aacgagatga
     5101 ccgtgcgcca cgtcctcacc cgcggcggct tctacgaggt cagcggcacc ggctaccgcc
     5161 ccgagggcga gatcaccctc gaaggcaagg tcgccagcct ggcgcaccac gaccacctgc
     5221 acgcgctgat cgaggtcgtc gcggtcgcca acgacaccca catcgccgaa gaggacggcc
     5281 agtggaaggt caccggcgag cccaccgagg gcgcgctgcg cacgctcgcg cacaaggccg
     5341 gcttcgacgc cggcgcgcac cagcgccacg acagcatccc cttcgagtcg gccaacaagt
     5401 tcatggccac gctcgacacc gccccctgcg ggcagcgccg catcctgctg aaaggcgcgc
     5461 ccgaccgcct gctcgagcgc tgccgccacg agctcggcgc cgacggcgag ctcgtggcgc
     5521 tcgaccgcgc cttctgggag gagcgcatcg acgcgctgag cggcgagggc ctgcgcgtgc
     5581 tcgccgccgc cgcgcgcgac gttcccgaca gcaaggacgc gctcgtcatc gaggacctgc
     5641 aggaggacat ggtcttcctc ggcctcatcg gcatcatcga cccgccgcgc ccggaagcga
     5701 tcgcggcgat ccgctcctgc cacgaagccg gcatccgcgt gaagatgatc accggcgacc
     5761 acgccggcac cgcgcgcgca atcggcatcg agatgggcct gggcacggcc gacgcgctga
     5821 aggtgatcac cggcgccgag atcgaggccg ccagcgacga ggaactgcag cggatcgccc
     5881 acgactgcga cctcttcgcg cgcaccagcc ccgagcacaa gctgcgcctg gtcgaggccc
     5941 tgcaggcgcg cggcgaggtg gtggcaatga ccggcgacgg ggtcaacgac gcccccgcgc
     6001 tcaagcgcgc cgacgtcggc gtggcgatgg gcatcaaggg caccgaggcc accaaggagg
     6061 ccgccgagat cgtgctcgcc gacgacaact tctccaccat cgagcgcgcc gtcgaggaag
     6121 gtcgcacgat ctacgacaac ctgcgcaagg ccatcctctt catcctgccc accaacggcg
     6181 cccagggctt cgtgatgctc gccgcggtgg tgctcggcct caccctgccg ctcacgccgg
     6241 tgcagatcct gtgggtgaac atggtggtgg cggtcacgct cgcgcttgcg ctcgccttcg
     6301 agcccgccga gccgggcgtc atgcagcgcc cgccgcggcg ccccggcgcg ccgatcatgg
     6361 acggcgcgat gctgtggcgg gtgggcttcg tgtcgctgct gatcggcggc gccaccatcg
     6421 tctccttcga gtattcgctg ctgcagggca tggaactgca ggccgcgcgc acgatggcgg
     6481 tcaacaccct ggtggtcgcg caggcctggt acctcttcaa cagccgcttc ctcaccgccc
     6541 ccagcctctc gcccgcgcgc ctgttcacca acccggcggc gcaggtctcg gtgctggtcc
     6601 tggccctgct gcagctcggc ttcgtctacc tgcccttcat gaacacctgg ttcggcacca
     6661 ccccgctcgc gctcgaatac tggctgcccc cgctcgcgat cggcgcgctg gtcttcctcg
     6721 tgatcgaggc cgagaaggcg ctgcagcggc gatacggggc gcgcaagagc ggctgagcgg
     6781 gcgcctcgcc aggcgccgat cgcgacttgc gtcgctcctg cagaaccgac acatcctcgg
     6841 atcgcctgca ggagcgacgc aagcgacttc gccgcgttcg acttctcccg cgcggggctc
     6901 ctctgcgggt ggtcgcggca agcctccccg tgcagctccg gtgcgggccg ctcgcgccac
     6961 gactcgcgca tccggtgcat gacgtcggcc gcaaggaggc ggacaatgtc ccgcacgccc
     7021 gcttcggccc accccaacca gacggagcat ccgatgagca agcacaaacc gatcgacatc
     7081 gccgagtttc gcgtcgaggg cggcaagaag gtcgacctcg ccgactggcc gaccaaggtc
     7141 cgcgccgtct acgactcgaa gagcgactac gaggagcgcc tggacgccgg caccgagcgc
     7201 ctggccgagc tgcaggagat gctctacgcc cacgaccgct actcgctgct ggtgatcttc
     7261 caggccatgg acgcggccgg caaggacggc gccatccgcc acgtcatgtc gggcatcaac
     7321 ccgcagggct gccaggtgtt cagcttcaag cacccgagcc ccaccgagct cgaccacgac
     7381 ttcctgtggc gcacccacgt cgcgctgccc gagcgcggcc gcatcggcat cttcaaccgc
     7441 tcgtactacg aggaggtgct gatcgtgcgc gtgctccccg agatcctgcg cggccagcag
     7501 ctgcccgacg agaccctggc cgcgcgcgac ttctggggcg aacgcttccg ctccatcgtc
     7561 gacgccgagg cccacctgca ccgcaacggc acgcgcatcg tcaagatttt cctgcacctt
     7621 tcgaaggacg agcagcgcga ccgcctgatc gcccgcatcg acgaggaaga caagaactgg
     7681 aagttcgacg aaggcgacat cgagcagcgc aagcactggg acaagtacat ggccgcctac
     7741 gccgactgcc tgcgcgccac cagcacgccc gattgcccat ggtacgtggt gccggccgac
     7801 gacaagaaga acgcccgcct gatcgtctcg cagatcctgg tcgacaccct cgcggggctc
     7861 gacctgcact accccgagac cagtccggcg cggcgcgaga agctcctcga gatccgccgc
     7921 cagctcgaag ccggcgaggt ctgacttcgc cggcctccgg atgcaccacg acgtcttgtg
     7981 ggagcgacgc gttgtgctgc gttccggccg cgttaccctt tgacgcgtcc ggatgcacca
     8041 cgacgtcttg tgggagcgac gcgagtcgcg attgcagcct gcgggagtcg tggcgcggac
     8101 ggaagcgccg cgatcgcgac ttgcgtcgct cccacaaaag accgcgcacc gccgtcgccg
     8161 cgacgcccac cccgggccgt gacagcgatc gatctcccat gggagcgacg cgagccgcga
     8221 cccctgcgcc ggagatccgc tcggcggtcc gtcgtgcgcc gcaccgcgac tggcgtcgcg
     8281 cctgtcgccc caggggcgcg gcggtttctt gtgggagcga cgcaagtcgc gactgcagcc
     8341 tgcgggagtc gtggcgcgga cggaagcgcc gcgatcgcga cttgcgtc
//
LOCUS       DAOQNN010000110         8345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4309_length_8345_cov_10.200483, whole genome shotgun sequence.
ACCESSION   DAOQNN010000110 DAOQNN010000000
VERSION     DAOQNN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8345
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4309_length_8345_cov_10.200483"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PKV97_07745"
     CDS             complement(<1..293)
                     /locus_tag="PKV97_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05807.1"
                     /translation="MPKTSSPRPLLLAGALALAAPAAFAADWLLATSSPQVVPGQRFE
                     VVVIGEGRAADWPARLPASIELSGGGPRIAVELVAIGKPAAGGGQRRYFARWP"
     gene            complement(428..2722)
                     /locus_tag="PKV97_07750"
     CDS             complement(428..2722)
                     /locus_tag="PKV97_07750"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNB05808.1"
                     /translation="MDQDFITAALDYHRSPTRGKISVVPTKGLTNQRDLALAYSPGVA
                     AACDAIVADPAEAFELTSRGNLVAVVTNGTAVLGLGNIGPLASKPVMEGKGCLFKKFA
                     NIDVFDIELAENDPDKLIEIIAALEPTLGGINLEDIKAPECFYIEKKLRERMKIPVFH
                     DDQHGTAIISAAGLVNGLKVVGKNIADVKLVCSGAGAAAIACLDMMVSVGLRRENIYV
                     CDSKGVIYEGREPNMEPTKARYAQKTEGRALGDVIAGADVFLGLSTAGVLKPEMVAKM
                     AAKPLIFALANPNPEILPADAKAVRPDCIVATGRSDFPNQVNNVLCFPFIFRGALDVG
                     ATTINEEMKLACVKAIAELAEAEASDIVASAYGGQELSFGSEYIIPKPFDPRLIVKIA
                     PAVAKAAMDSGVATKPIADLEAYASSLNAFVYQSGIVMKPVFSAAKAVPLEQKRVIYA
                     EGEDERVLHAVRVVLDEGLARPILIGRPEVIEMRIKKIGLNLKPERDFEIVNPESDPR
                     YRELWTEYYKLMKRDGVTPDLAKAKIRRDTTLIGVMMLRRGDADALVCGTFGSYDYHL
                     KQVADVIGLAPGAKQFAAMNLLLLTKRMLAITDTYVNENPSAEEVAEIARMASDELRR
                     FGIEPSVALLSHSNFGSSNSASARKMRRACEILRETSPDLNVDGEMHGDAALSEEIRD
                     KLHPDSGLKGEANLLVMPNLDAANISFNLMKVANGDGVSVGPILLGAAKPVHIVTPSA
                     TVRRLVNMTALAVVDAKESRNAGA"
     gene            3073..3852
                     /locus_tag="PKV97_07755"
     CDS             3073..3852
                     /locus_tag="PKV97_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNB05809.1"
                     /translation="MRRVVFNQKGGVGKSTITCNLAAIAAAQGKRTLVVDLDPQGNST
                     QYLLGAGVDVLDATLADFFDQTLNFKLNPRGTKDFVVATPFERLHVMPSHPQLEELQS
                     KLESRYKIYKLRDALEELAKDFDCVFIDTPPALNFFTRSALIAADACLIPFDCDEFSR
                     KALYSLLANVEEIKADHNRALEVEGIVVNQFQPRASLPQKVVQELIDEGLPVLQPYLS
                     ASIRIKESHEQAKPMIHLDPKHKLSQEFVALHDTLARKYGT"
     assembly_gap    3989..4088
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4308..5429)
                     /locus_tag="PKV97_07760"
     CDS             complement(4308..5429)
                     /locus_tag="PKV97_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNB05810.1"
                     /translation="MIDQVAEKVVRRVILGFLLGGLLLLSYAVLHLFIVPVAWAVIIA
                     YATWTPYRQLRSRLPRYPTISALLMTLLLSAAFVLPALWIGMLLRTEVGVAIAAVTAQ
                     IREGAFVLPEFVRTLPWIGDDLQAMVGELTREPEALRAQLTEWVRQGSDLALTLIGDV
                     GRNAAKLGFALITVFFLYRDGERVLEQVVAVLRRFLGERLDPYLSAVGGMTKAVVWGL
                     IATAIGQGFVAGLGYWWAGVPAPVLMGAITAVIAMIPFGTPFAWGSIGAWLLLTGNTV
                     EGIGLLLWGALVVSWVDNLVRPLVISNATRIPFLLVMFGVLGGLSAFGLVGLFLGPVV
                     LAVLMAVWGEWLEESEFARLAAIGAPAGAREEVERGGRS"
     gene            complement(5426..6769)
                     /locus_tag="PKV97_07765"
     CDS             complement(5426..6769)
                     /locus_tag="PKV97_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNB05811.1"
                     /translation="MEILFLIGLIVLNGVFAMSEIALVTARRARLARLAEEGDGAAEV
                     AIKLGEDPTRFLSTIQIGITSIGVLNGIVGEAALAGPLSDWLQVLGMEQRVSEIGSTV
                     LVVVVITYVSIVVGELVPKRIGQIDPEGIARLVARPMNVLSIASRPFVYLLAGSTALL
                     LRIMGQRESTGPSVTEEEIHAMLVEGSEAGVIEKSEHDMVRNVFRLDDRQIGSLMVPR
                     ADIVSLDLERPLEENLELVASSSYSSFPVCRGGLDDILGIASAKKLFNQSLRGEPIDL
                     ARELQPAVYVPESLTGMELLDQFRSSGTYTVFVIDEYGEIQGMVTLHDVIESVTGEFL
                     PHDTEEAWAVQREDGSWLLDGLIPIVELKDRLGIKTVPEEEKGRYHTLSGMVMWLLGR
                     LPGTGDIATWENWRFEVIDLDGKRIDKVLASRLPEPADEIGAEQEASPEDRGETA"
     gene            7192..8169
                     /locus_tag="PKV97_07770"
     CDS             7192..8169
                     /locus_tag="PKV97_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNB05812.1"
                     /translation="MESIGTWWMWAAFAAIVGTMLLVDLFAFGGGKQHRVSLKEAAGW
                     SLAWVTVAMLFAAGLWWYLDGTLGRAVANEQTTAFVTGYLVEKSLAVDNVFVWMMIFS
                     FFAVPLELQRRVLLYGIVGAIVLRTIMIFAGSWLISEFHWLLYVFGAFLIVTGIKMAW
                     FSEHDPDLDKNPLIRWIRNHYPITEKLEGERFFVVRDGVRMMTPLFLALVLVEISDVI
                     FAVDSIPAIFAITTDPFIVLTSNLFAILGLRAMYFLLADLGDRFELLKYGLAAILVFI
                     GTKMLIVEWVKIPVLVSLSVVATILACSVFASLWYTARKEKEAARINAG"
BASE COUNT         1350 a         2834 c         2744 g         1317 t
ORIGIN      
        1 atcggccagc gtgcgaaata gcggcgctgg ccgcccccgg ccgccggctt gccgatcgcg
       61 accagctcga ccgcgatgcg cggcccgcca cccgacagct cgatgctcgc cggcagccgc
      121 gccggccaat ccgccgcgcg gccctcgccg atcaccacga cttcgaagcg ctggcccggc
      181 acgacctgcg ggctggaggt ggcgagcagc cagtcggcgg cgaacgcggc cggcgcggcc
      241 agggcgagcg cgccggcgag cagcagcggg cgcggggagg aagttttcgg catggcgatc
      301 gcctggcggg aacgaaggaa acaaagcggg gcagcccctc gcaggactgc ccccgggaaa
      361 acgccgcgcg aacctcctcg tgcgaggctc gtgcggaggc ggcgccgagc ggcgccgccg
      421 gcaggcatca ggccccggcg ttgcgcgact ccttggcatc gaccacggcg agcgcggtca
      481 tgttgaccag gcggcgcacg gtggccgagg gggtcacgat gtgcaccggt ttggccgcgc
      541 ccagcaggat cggacccacc gagacgccgt cgccattggc caccttcatc aggttgaacg
      601 agatgttggc ggcgtccagg ttgggcatca ccagcaggtt ggcctcgccc ttcaggcccg
      661 aatccggatg cagcttgtcg cggatctcct cggagagcgc ggcatcgccg tgcatctcgc
      721 catcgacgtt gaggtcgggc gaggtctcgc gcaggatctc gcaggcgcgg cgcatcttgc
      781 gcgccgaagc cgagttcgag ctgccgaagt tggaatgcga caacagcgcc acgctcggct
      841 cgataccgaa gcggcgcagt tcgtcggacg ccatgcgggc gatctcggcg acttcctccg
      901 cactcgggtt ctcattcaca taggtgtcgg tgatcgccag catgcgcttg gtgagcagca
      961 gcaggttcat cgccgcgaac tgcttggcgc ccggcgccag cccgatcacg tccgccacct
     1021 gcttgaggtg gtagtcgtag gagccgaagg tgccgcacac cagcgcatcg gcatcgccgc
     1081 ggcgcagcat catgaccccg atcagggtgg tgtcgcggcg gatcttggct ttggccaggt
     1141 cgggggtgac cccgtcgcgc ttcatgagct tgtagtactc ggtccacagc tcgcgatagc
     1201 gcgggtcgga ctccggattc acgatctcga agtcgcgctc gggcttgagg ttgaggccga
     1261 tcttcttgat ccgcatctcg atgacctcgg ggcgaccgat gaggatcgga cgcgccaagc
     1321 cttcgtccag caccacgcgc accgcgtgca gcacgcgctc gtcctcaccc tcggcgtaga
     1381 tcacgcgctt ctgctccagc ggcaccgcct tggcggccga gaacaccggc ttcatgacga
     1441 tgccggactg gtagacgaag gcgttgaggc tggaggcgta ggcctcgagg tcggcgatcg
     1501 gcttggtcgc cacgcccgaa tccatcgccg ccttcgccac cgcgggcgcg atcttgacga
     1561 tcaggcgcgg atcgaagggc ttcgggatga tgtactcgga accgaagctg agctcctggc
     1621 cgccgtaggc gctggcgacg atgtcgctcg cctcggcctc ggccagctcg gcgatcgcct
     1681 tcacgcaggc gagcttcatc tcctcgttga tcgtggtcgc gccgacgtcg agcgcacccc
     1741 ggaagatgaa ggggaagcac agcacgttat tgacctggtt cgggaagtcc gaacggcccg
     1801 tggccacgat gcagtcggga cgcacggcct tggcgtcggc cggcaggatc tcggggttcg
     1861 ggttggcgag cgcgaagatc agcggcttgg cagccatctt cgccaccatc tcgggcttga
     1921 gcacgcccgc ggtcgacaag cccaggaaga cgtcggcgcc ggcgatcacg tcgcccagcg
     1981 cgcggccctc ggtcttctgc gcgtagcgcg ccttggtcgg ctccatgttg ggctcgcggc
     2041 cctcgtagat gacgcccttg gagtcgcaca catagatgtt ctcgcggcgc aggcccacgc
     2101 tgaccatcat gtcgaggcag gcgatcgccg cggcaccggc gccggagcac accagcttga
     2161 cgtcggcgat gttcttgccg accaccttga gaccgttgac caggccggca gccgaaatga
     2221 tcgcggtgcc gtgctggtcg tcgtggaaga cggggatctt catgcgctcg cgcagcttct
     2281 tctcgatgta gaagcactcg ggcgccttga tgtcctcgag gttgatgccg ccgagcgtgg
     2341 gctcgagcgc ggcgatgatc tcgatcagct tgtcggggtc gttctcggcc agctcgatgt
     2401 cgaagacgtc gatgttggcg aacttcttga acaggcaacc cttgccttcc atgaccggct
     2461 tggaggcgag cgggccgatg ttgcccaggc cgagcaccgc ggtgccgttg gtgaccacgg
     2521 cgacgaggtt gccgcgactg gtgagctcga aggcttcggc agggtcggcg acgatcgcgt
     2581 cgcaggccgc ggccacgccg ggcgaatagg cgagcgcgag atcgcgctgg ttggtgaggc
     2641 ccttggtggg caccaccgag atcttgccgc gcgtcggcga gcggtgatag tcgagcgctg
     2701 cggtgatgaa atcctgatcc atgttctccc ctctcgtgtc tatgaggtaa tcgatatgag
     2761 gtcgtcgagg ccgggccgga atcgcgacgc agcacccgcc cgcccggggc ttgcagcccg
     2821 ggacggagga cctgctcggg aatcgaacgc agacctgctt gttcttgttg gcgtcgtccg
     2881 gctcgggatc gccagcgacc gacgcccccg aagtgtactc cagcaccccg gccttggaca
     2941 cgcccgccgc ttatgcgcag gatcaccctg gaatcgagct ttgccggtgc ctggcgcgcc
     3001 ggaggcgccg gtttttggca gaatgcgggg ccggcgagca gccgcgcgca catgacgaca
     3061 ggggagaagg cgatgcgacg ggtggtattc aatcagaagg gcggtgtcgg caagtcgacg
     3121 atcacctgca acctcgcggc gatcgccgcc gcccagggca agcgcaccct ggtggtggac
     3181 ctcgacccgc agggcaactc cacccagtac ctgctcggtg ccggcgtgga cgtcctggat
     3241 gcgacgctcg ccgatttttt cgaccagacg ctcaacttca agctcaaccc gcgcggcacc
     3301 aaggacttcg tcgtcgccac ccccttcgag cgcctgcacg tgatgccctc gcatccgcag
     3361 ctcgaggagc tgcaatccaa gctcgagtcg cgctacaaga tctacaagct gcgcgatgcg
     3421 ctggaggagc tggcgaagga cttcgactgc gtcttcatcg acaccccccc ggcgctcaac
     3481 ttcttcaccc gctcggcgct gatcgccgcc gatgcctgcc tgatcccttt cgactgcgac
     3541 gagttctcgc gcaaggcgct gtactcgctg ctcgcgaacg tggaagagat caaggccgac
     3601 cacaaccgcg ccctcgaggt cgagggcatc gtggtcaacc agttccagcc gcgcgcgagc
     3661 ctgccgcaaa aggtggtcca ggaactcatc gacgagggtc tgccggtgct gcagccctac
     3721 ctgtcggcct cgatcaggat caaggaatcg cacgagcagg ccaagccgat gatccacctc
     3781 gaccccaagc acaagctgtc gcaggagttc gtcgcgctgc acgacaccct ggcgcgcaag
     3841 tacggcacct gagcctcgcg ctttccgcag gagcgacgcg agtcgcgatc ccggcgcgaa
     3901 ccgggcgccg tgccgacgga accgtggcaa tcgcgacttg tgtcgctcct acggggcgac
     3961 cgcgcgcttc gacgtttcct gtaggagcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnngc cgtgccgacg gaaccgtggc aatcgcgact tgcgtcgctc ctacggggcg
     4141 accgcgcgct tcgacgtttc ctgtaggagc gacgcgagtc gcgatcccgg cgcgaaccgg
     4201 gtgccgtgcc gacggaaccg tggcaatcgc gacttgcgcc gcctcctacg gggcgaccgc
     4261 gcgcttcgac gtttcctgta ggagcgacgc gagtcgcgat cggcggctca ggaccgaccg
     4321 ccgcgctcca cctcctccct cgcgcccgcg ggcgctccga tcgccgcaag gcgcgcgaac
     4381 tccgactcct ccagccactc gccccacacc gccatcagca ctgccagcac caccggcccg
     4441 aggaacaggc cgaccagccc gaacgcggac agcccgccga gcacgccgaa catcaccagc
     4501 aggaagggga tgcgggtggc attgctgatc accagcggac gcaccaggtt atccacccag
     4561 ctcaccacca gcgcgcccca cagcagcagc ccgatgccct cgacggtgtt gccggtgagc
     4621 agcagccagg cgccgatcga gccccacgcg aagggcgtgc cgaaggggat catcgcgatc
     4681 acggcggtga tcgcccccat cagcaccggt gccggcacgc ccgcccacca gtagcccagc
     4741 ccggcgacga aaccctggcc gatcgcggtc gcgatcaggc cccacaccac cgccttggtc
     4801 atcccgccca ccgcggagag ataggggtcc aggcgctcgc cgaggaagcg gcgcagcacc
     4861 gccaccacct gctcgagcac gcgctcgccg tcgcggtaga ggaagaacac ggtgatgagt
     4921 gcgaaaccca gcttggccgc gttgcgcccg acgtcgccga tcagcgtcag cgcgaggtcg
     4981 ctgccctggc gcacccactc ggtgagttgc gcgcgcagcg cctccggctc gcgggtgagc
     5041 tcgcccacca tcgcctgcag gtcgtcgccg atccacggca gcgtgcggac gaactcgggc
     5101 agcacgaagg cgccctcgcg gatctgcgcg gtcaccgcgg cgatcgcgac accgacctcg
     5161 gtgcgcagca gcatgccgat ccacagcgcg ggcagcacga aggcggcgct cagcaaaagc
     5221 gtcatcagca gcgcgctgat cgtgggatag cgcggtaacc gcgagcgcag ctggcggtaa
     5281 ggcgtccacg tggcataggc gatgatcacc gcccacgcca ccggcacgat gaacaggtgc
     5341 agcaccgcat agctcagcaa cagcaggccg ccgagcagga agccgaggat gacgcggcgg
     5401 acgaccttct ccgcgacctg gtcgatcatg cggtctcgcc ccgatcttcc gggctggcct
     5461 cctgctcggc gccgatctcg tcggccggct cgggcaggcg gctcgccagc accttgtcga
     5521 tacgcttgcc gtcgaggtcg atcacctcga agcgccagtt ctcccaggtg gcgatgtcgc
     5581 cggtgcccgg caggcggccg agcagccaca tcaccatgcc cgacagcgtg tggtagcgcc
     5641 ccttctcctc ttccggcacg gtcttgatgc ccaggcggtc cttgagctcg acgatcggga
     5701 tcaggccgtc gagcagccag gagccgtcct cgcgctgcac cgcccaggcc tcctcggtgt
     5761 cgtgcggcag gaactcgccg gtcaccgact cgatcacgtc gtgcagcgtg accatgccct
     5821 ggatctcgcc gtactcgtcg atcacgaaca cggtgtaggt gccggaagag cggaactgat
     5881 cgagcagctc catgccggtc agcgactcgg gcacgtacac cgcaggctgc agctccctgg
     5941 cgaggtcgat cggctcgccg cgcagggact ggttgaagag cttcttggcg ctggcaatgc
     6001 cgaggatgtc gtcgagcccg ccgcggcaca ccgggaagct ggagtaggac gaggacgcca
     6061 ccagctcgag gttctcctcc agcggccgct cgaggtcgag cgacacgatg tccgcgcgcg
     6121 gcaccatcag cgagccgatc tggcggtcgt cgaggcggaa cacgttgcgc accatgtcgt
     6181 gctcgctctt ctcgatcacg ccggcctcgg agccctcgac cagcatggcg tggatctcct
     6241 cctcggtcac gctcggcccg gtgctctcgc gctggcccat gatgcgcagc agcagcgcgg
     6301 tggagccggc gagcaggtag acgaagggcc gcgaggcgat cgacagcacg ttcatcggcc
     6361 gcgccaccag gcgggcgatc ccctcggggt cgatctgccc gatgcgcttg ggcaccagct
     6421 cgccgaccac gatcgacaca taggtgatca ccaccacgac cagcacggtg gagccgatct
     6481 cgctcacccg ctgctccatg ccgagcacct gcaaccagtc ggagagcggg ccggcgagcg
     6541 cggcctcgcc gacgatgccg ttgagcacgc cgatcgaggt gatgccgatc tggatcgtcg
     6601 acaggaagcg ggtcgggtcc tcgccgagct tgatcgccac ctcggccgcg ccgtcgccct
     6661 cctcggcgag ccgcgcgagc cgtgcccgac gcgcggtgac gagcgcgatc tcggacatgg
     6721 cgaagacgcc gttgaggacg atgaggccga tcagaaagag gatttccatg ggttcgaggt
     6781 ggttgagaga ggggcgccgg cgggcccccg ggcatgacgg gcgggccgaa cgccgccgat
     6841 gatgccccag cggacacggg gtgtcgaccg cgcgaccacg cgccacgctc gcccgctgct
     6901 gcagcgcaac accacgaacc ccttgctcgc ggcgacccga aacgccggcg ttgacactcc
     6961 cgcagtgcaa cagacacaat cccgttcatc aaggggagta gcttttccgc tccggcatcg
     7021 tcaatacggc agatcggtct tcagaccgtc tcccggtgcc gggggcaacg aacggatcca
     7081 ttcgttcgtt gcaagcgaga ccttgcccga tgggcaaggt ccgtgcatcg cagcgcgcca
     7141 gggtctgtgt ccgtcgggaa cagacctttt ttgttttctg cggagcgcgc gatggaatcg
     7201 atcggaacgt ggtggatgtg ggcggccttt gccgccatcg tgggaacgat gctgcttgtc
     7261 gacttgttcg ccttcggtgg cggcaagcaa caccgggtgt cgttgaagga ggcggcaggc
     7321 tggtcgctcg cctgggtcac cgtggcgatg ctgttcgccg ccgggctgtg gtggtatctc
     7381 gacggcacgc tcgggcgtgc ggtcgccaac gagcagacga ccgccttcgt caccggctac
     7441 ctggtcgaga agtcgctggc ggtcgacaac gtcttcgtgt ggatgatgat cttcagcttc
     7501 ttcgcggtgc cgctcgagtt gcagcgccgc gtgctgctgt acggcatcgt cggcgcgatc
     7561 gtgctgcgca cgatcatgat cttcgcaggc agctggctga tcagcgagtt ccactggctg
     7621 ctttacgtct tcggcgcctt cctgatcgtc accggcatca agatggcgtg gttctccgag
     7681 cacgaccccg acctggacaa gaacccgctc atccgctgga tccgcaatca ctacccgatc
     7741 accgagaagc tcgagggcga gcgcttcttc gtcgtgcgcg acggcgtgcg catgatgacg
     7801 ccactctttc tcgccctggt gctggtcgaa atctccgacg tgatcttcgc ggtcgacagc
     7861 atcccggcga tcttcgcgat caccaccgac cccttcatcg tgctgacctc gaacctgttc
     7921 gccatcctcg gcctgcgcgc gatgtacttc ctgctcgccg acctcggcga ccgcttcgag
     7981 ctgctcaagt acggactggc ggccatcctg gtcttcatcg gcaccaagat gctgatcgtg
     8041 gagtgggtga agatcccggt gctggtctcg ttgtcggtgg tcgccaccat cctggcctgc
     8101 tcggtgttcg cgagcctgtg gtacacggcg cgcaaggaga aggaagccgc caggatcaac
     8161 gcgggctgac gcgcagcggg cgggttgggg gcgccgggcg ccgcgtcgcc gcccccatcc
     8221 cgccatcgcg acttgcgtcg ctcccacgag aaccagcccg aaatgtcggg ctgcacggta
     8281 ggagcgacgc aagtcgcgat cccgccggaa aagcaggtgc cgccccccct gaatccccta
     8341 cccct
//
LOCUS       DAOQNN010000111         8340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4314_length_8340_cov_8.580688, whole genome shotgun sequence.
ACCESSION   DAOQNN010000111 DAOQNN010000000
VERSION     DAOQNN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4314_length_8340_cov_8.580688"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..835)
                     /locus_tag="PKV97_07775"
     CDS             complement(17..835)
                     /locus_tag="PKV97_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB05813.1"
                     /translation="MSSERGTSIRALERGIEVLRLLGPQEGSSLRALHAASALPKPTL
                     LRILHTLERGGLAWRSIDDGLWRRSLAWTAAPSGHAIDPRLVEAAAPHLVALQRTVLW
                     PSDLLVYRDFAMEVAESSRRLSGLALNPRYRIGYRVDILLSAPGRAWLAFCAAAEREA
                     VLAHWRAASPSNPRTAAVLEGEIDALLEETRRRGYAVRDALFGGSFEDMAGFDDGFDA
                     IAVPILADGGVRGAINLVWPRRYDLRAKVVEAHLADLQACAAAIAAALASPRQP"
     gene            complement(876..1343)
                     /locus_tag="PKV97_07780"
     CDS             complement(876..1343)
                     /locus_tag="PKV97_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNB05814.1"
                     /translation="MRDLDRTDLKILDLLQKNGRLTMTELAHQVGLSATPCTERVRRL
                     EREGVITGYHARVDPRALGRPLLVFVELKLAAKSNDAFERVKKELAFVPEVMECHLVS
                     GDFDYLIKARISEMSDYRRLLGNILLKLPSATESRSYVVMEEVKESLYIPPMS"
     gene            1477..2802
                     /locus_tag="PKV97_07785"
     CDS             1477..2802
                     /locus_tag="PKV97_07785"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HNB05815.1"
                     /translation="MNVIVLGSGVIGTTTAWYLAQRGARVTVIDRQPGAALETSYANA
                     GQVSPGYSTPWAAPGIPLKALKWLFQRHAPLAIRADGSLFQLRWMAQMLANCSSARYA
                     VNKERMMRVSEYARDCLRTLRADTGIAYEERSRGTLQLFRTQAQLDAAARDIAVLEEC
                     GVPYELLDGDALASAEPALARRPGKLAGGLRLPNDETGDCQLFTTRLAAMAEAAGVTF
                     RFGTEVRELVRRGGHIEGVRIAGADGVEHTLVADRYVLAFGSYSRALAASLGLDLPVY
                     PVKGYSLTVPLVDAARAPVSTVLDETYKVAVTRFDERIRVGGMAELGGFDLRLNPRRR
                     ETLELVVNDLFPGGGDVKAAEFWTGLRPMTPDSTPIIGATPFPELFLNTGHGTLGWTM
                     SCGAGALVADLVVGRQPAIRHDDLGLARYGAEHRSIPVAGARQRPLGTV"
     assembly_gap    2986..3085
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3309..4394
                     /gene="alr"
                     /locus_tag="PKV97_07790"
     CDS             3309..4394
                     /gene="alr"
                     /locus_tag="PKV97_07790"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNB05816.1"
                     /translation="MRPARALIDLSALRHNYLLARERHGGRALAVVKANAYGHGALRC
                     AQALRDEADGFAVAFLGEALELRAGGIDAPILLLEGVFAADELAEVARQRLWMVVHHE
                     EQLCMLERTPLAAPVDVWLKVNSGMNRAGFVGDAVARAWRRLQASGKVGRIVLMTHFA
                     RADEPELGTTLEQLQRFEAATAGLPGARSVANSAAILAWPQARRDWARPGILLYGADP
                     MPAEGNGLRAVMTLESAVMAVREIAPGEALGYGGRFVAERPTRVGLVAMGYADGYPRV
                     VPDGTPVAVEGRASRIIGRVSMDMLTIDLTALPEAGLGSQVELWGKQVPINHIAQAAG
                     TISYELLCNVKRVRLEYADARGVGGKA"
     assembly_gap    4510..4609
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4633..5856)
                     /gene="ccmI"
                     /locus_tag="PKV97_07795"
     CDS             complement(4633..5856)
                     /gene="ccmI"
                     /locus_tag="PKV97_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HNB05817.1"
                     /translation="MTAFLVFAGLLVAGALLLILPPLLGYGRRGQAQMRQSAMVLTVL
                     REQLADLDAELAEGRIDAATHAKSREELERRALEEAEVAAETADSADRRPSKAWALAT
                     GLAIPALAVGTYLMIGEPAALDPKNVEGQQGFTQEQVTEMVGSLEKHLAANPDNAEGW
                     AMLARTYLVLKDFPKAAAAYERLDKLVPNDPDVLSDWADVVAAQTGKVGSDAEALVLR
                     ALEAEPKHPKALALAGTAAYQRDDFAAAAGYWERILALIPPGDDVARGVRASINEARA
                     KAGMAPLATNEGNAAPPPASPLKVAGRLQVEGALAGQVAAEDAVFVFVRGPAGGPPLA
                     ALRFKGSELPVNFDFDGARLMMGDAAVPERVVIAARVSKGGDATARPGDLEGVSAPVA
                     PDAEGVTVTIDRVRE"
     gene            complement(5853..6332)
                     /locus_tag="PKV97_07800"
     CDS             complement(5853..6332)
                     /locus_tag="PKV97_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNB05818.1"
                     /translation="MTPAGIATASDPGVAPAVAADPKLEADMMELSHKLRCLVCQNQS
                     IAESNAPLAVDLRNQVREQLAAGKDKDDVIDYLVERYGDFVLYEPPFKAVTGLLWVGP
                     FALLLGGAGWLAWRLRRRRSEIAAEGALSAADRERARALLAGGGDAASNPASPESRK"
     gene            complement(6448..7041)
                     /locus_tag="PKV97_07805"
     CDS             complement(6448..7041)
                     /locus_tag="PKV97_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HNB05819.1"
                     /translation="MKAKFLVPLLLFFGLAGFLAFGLTLNPREVPSPLIDKPAPNFRL
                     ARLDQPEQTFALEEMRGQVWLLNVWASWCVACRQEHPVLVRMAAQKLVPVVGLNYKEV
                     RGDGAISTRGMALEAETTMAIERARRWLADHGNPYLLSVLDIDGRVGIDFGVYGVPET
                     FLIDREGRIRYKHIGPITPESLQTQIMPKVEELRRAG"
     gene            complement(7060..>8340)
                     /locus_tag="PKV97_07810"
     CDS             complement(7060..>8340)
                     /locus_tag="PKV97_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNB05820.1"
                     /translation="LGWGGWWFWDPVENASFMPWLLGTALMHSLAVTEKRGAFRSWTV
                     LLAIGAFSLSLLGTFLVRSGVITSVHAFATDPRRGLYILALLVIVIGFSLLLYAMRAN
                     KLMGGGSFGLVSRETALLANNVLLTVASASVLLGTLYPLFLDALGLGKISVGPPYFEA
                     VFVPLMTPVVVLMVFGPFLRWKDDELLAEVRRVAPAFVASVAIGVGSAWAVDHLTWRT
                     ALGLSLAAWVVLASLQLLVRRLGERAGASAGSRLRSITASWWGMWLAHFGIGIFIIGV
                     TMVGSLDRHLDVKMKEGQHAELAGYTFVFRGAVDADGPNYDAARGRIELLRDGRSLDM
                     LTPEKRVYLAQGMPMTEASLDIGVFRDVYVSLGEQLEDGAWIVSLYYKPFISWIWAGC
                     VLMGLGGVFAAADRRYRRLAARDAAAAVGSRVAA"
BASE COUNT         1182 a         2886 c         2953 g         1119 t
ORIGIN      
        1 gatggcgcgg cggtgctcag ggctggcgcg gcgacgcgag cgcggccgcg atcgccgcgg
       61 cgcaggcctg caggtcggcc agatgcgcct cgaccacctt cgcgcgcagg tcgtagcgcc
      121 gcggccacac caggttgatc gccccgcgca ccccgccatc ggcgaggatc ggcacggcga
      181 tggcgtcgaa gccgtcgtcg aagcccgcca tgtcctcgaa cgagccgccg aagagcgcgt
      241 cgcgcaccgc atagccgcgc cgacgcgtct cctccaggag ggcgtcgatc tcgccctcga
      301 ggaccgccgc cgtgcgcgga ttcgacggcg aggcggcgcg ccagtgggcg agcaccgctt
      361 cgcgttcggc cgcagcgcag aaggcaagcc aggcccgccc gggcgcggag agcaggatgt
      421 ccacccggta gccgatgcgg tagcgcggat tgagcgccag cccggagagg cggcgggagc
      481 tctccgcgac ctccatcgcg aagtcgcggt acaccagcag gtcggacggc cacagcaccg
      541 tgcgctgcag cgccaccagg tgcggcgccg cggcctcgac caggcgcgga tcgatcgcgt
      601 gcccgctcgg cgccgcggtc cacgccagcg agcgccgcca cagcccgtcg tcgatcgatc
      661 gccacgccag cccgccccgc tccagggtgt gcaggatgcg cagcagggtc ggcttgggga
      721 gcgcgctggc ggcgtggagc gcgcgcagcg agctgccctc ctgggggccg agcaggcgca
      781 gcacctcgat gccgcgttcg agggcacgga tcgaggtgcc acgttccgaa ctcatgcgtg
      841 gcggctcctg tcgtccatcc gggacctccc gcggctcatg acatgggagg aatatagagg
      901 ctctccttca cctcctccat caccacgtag ctgcgcgact cggtcgccga gggcagcttg
      961 agcaggatgt tgccgagcag gcgacggtag tcgctcatct ccgaaatgcg cgccttgatc
     1021 aggtagtcga agtcgcccga cacgaggtgg cactccatga cctcggggac gaaggcgagc
     1081 tccttcttca cgcgctcgaa ggcgtcgttc gacttggcgg cgagcttgag ctcgacgaag
     1141 accagcagtg ggcggccgag cgcgcgcggg tcgacgcggg cgtggtagcc ggtgatcacg
     1201 ccctcgcgct ccaggcggcg cacacgctcg gtgcacgggg tcgccgacag gcccacctgg
     1261 tgggcgagct cggtcatcgt caggcgcccg ttcttctgca acaaatccag tattttcagg
     1321 tctgtacggt cgaggtcgcg catttcactg cgccgccggc aaggcggcct ccggattggt
     1381 gatcttcact gattggcgat atcagtttag tgactttcgc tagcaaggcc tcattagact
     1441 gccgcaattc catcgaatac aggcaggaga cgcgaaatga acgtgatcgt actcggcagc
     1501 ggcgtgatcg gcaccaccac cgcgtggtat ctggcccaac gcggcgcgcg cgtcaccgtg
     1561 atcgaccgcc agcccggcgc cgcgctcgag accagctacg ccaacgccgg ccaggtctcg
     1621 cccggctact ccacgccctg ggccgcgccc ggcattccgc tgaaggcgct gaagtggctg
     1681 ttccagcgcc acgccccgct cgcgatccgc gccgacggca gcctgttcca gctgcgctgg
     1741 atggcgcaga tgctcgccaa ctgctcgagc gcgcgctacg cggtgaacaa ggaacgcatg
     1801 atgcgcgtct ccgagtacgc ccgcgactgc ctgcgcacgc tgcgcgccga caccggcatc
     1861 gcctacgagg agcgcagccg cggcacgctg cagctgttcc gcacccaggc ccagctcgac
     1921 gccgccgcgc gcgacatcgc ggtgctggag gagtgcggcg tgccctacga gctgctcgac
     1981 ggtgacgcgc tcgccagcgc cgaaccggcg ctggcccgtc gccccggcaa gctcgccggt
     2041 ggcctgcgcc tgcccaacga cgagaccggc gactgccagc tcttcaccac ccgcctggcg
     2101 gcgatggcgg aggcggccgg cgtgacgttc cgcttcggca ccgaggtgcg cgagctggtg
     2161 cgccgcggcg gccatatcga gggcgtgcgc atcgccggtg ccgacggcgt cgagcacacg
     2221 ctggtcgccg accgctacgt gctcgccttc ggcagctact cgcgcgcgct cgccgccagc
     2281 ctcggactgg acctgccggt gtatccggtg aagggctact cgctgaccgt gccgctggtc
     2341 gacgccgcgc gcgcgccggt gtccaccgtg ctcgacgaga cctacaaggt cgcggtgacg
     2401 cgcttcgacg agcgcatccg cgtcggcggc atggccgagc tcggcggctt cgacctgcgc
     2461 ctcaacccgc gccggcgcga gaccctggag ctggtggtga acgacctctt ccccggcggt
     2521 ggcgacgtga aggcggcgga gttctggacc ggcctgcgcc cgatgacgcc ggactccacc
     2581 ccgatcatcg gtgcaacgcc cttccccgag ctcttcctca acaccggcca cggcaccctg
     2641 gggtggacga tgtcctgtgg cgccggcgca ctcgtcgccg acctggtcgt cggccgccag
     2701 ccggcgatcc gccacgacga cctcggcctc gcgcgctacg gcgccgagca ccgcagcata
     2761 ccggtggccg gcgcccgcca gcgtccgctc ggcaccgtct gaagcgcgct tgcgcggctt
     2821 cgtgagccac ggcgcgtacc cggggggctg catcgcgact cgcgtcgctc ctacagtgcc
     2881 ccggcagcgt gggcggcccc tgtaggagcg acgcaagtcg cgattccacg gctccatgtg
     2941 ctgcggcggt gatccgcgcg cccgatcgcg acttgcgtcg ctcctnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnngctcc atgtgctgcg gcggtgatcc gcgcgcccga
     3121 tcgcgacttg cgtcgctcct acggcgcggc tccggcagcg tggcgggccc tcgtgggagc
     3181 gacacgagtc gcgattccac ggcgcgggag cctctgtggg agcgacgcga gtcgcgatcg
     3241 ggccgcgcat tgcggcgctg gcgaattccc cgcaccattt tgcctttcca ccccttccga
     3301 tccccgccat gcgtcccgcc cgcgccctca tcgacctctc cgccctgcgc cacaactatc
     3361 ttctcgctcg cgagcgccac ggcgggcggg cgctcgcggt ggtcaaggcc aacgcctatg
     3421 gccacggcgc gctgcgctgc gcgcaggcct tgcgcgacga ggccgacggc ttcgcggtag
     3481 ccttcctcgg cgaggcgctg gagctgcgcg cgggcggcat cgatgccccc atcctgctgc
     3541 tcgagggcgt gttcgccgcc gacgagctcg ccgaggtggc gcgccagcgg ctgtggatgg
     3601 tcgtgcacca cgaggagcag ctgtgcatgc tggagcgcac gccgctcgcc gcaccggtgg
     3661 atgtgtggct gaaggtcaat agcggcatga accgcgccgg tttcgtcggc gacgcggtgg
     3721 cgcgggcctg gcggcgcctg caggcgagcg gcaaggtcgg acgcatcgtg ctgatgacgc
     3781 acttcgcgcg cgccgacgag cccgagctcg ggaccacgct ggaacagttg cagcgcttcg
     3841 aggccgccac cgccggcctg cccggcgcgc gcagcgtggc caactcggca gcgatcctgg
     3901 cctggccgca ggcgcgccgc gactgggcgc gcccgggcat cctgctctat ggggccgatc
     3961 ccatgcccgc cgagggcaac ggcctgcgtg cggtgatgac gctggagagc gcggtgatgg
     4021 cggtgcgcga gatcgcgccc ggcgaggcgc tcggctacgg cggccgcttc gtcgccgaac
     4081 gcccgacgcg ggtcggcctg gtggcgatgg gctacgccga cggctacccg cgcgtggtgc
     4141 ccgacggcac cccggtggcg gtggagggcc gcgcgagccg gatcatcggc cgggtgtcga
     4201 tggacatgct gacgatcgac ctcaccgcgc tgcccgaggc cggcctgggc agccaggtcg
     4261 agctatgggg caagcaggtg ccgatcaacc acatcgccca ggccgcgggc acgatcagct
     4321 acgagctgct gtgcaacgtg aagcgggtgc ggctggagta tgcggacgcg cgaggcgtgg
     4381 gaggcaaggc gtagggagcg aggcgtcagc tgcggcgggc gatcgagcgt ccaccggctt
     4441 cctgaagcct ctcagcgcag cttgcggagg ttttgctcct tgcccccgac gcctcgcacc
     4501 cgacgcctcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ccgacgcctc
     4621 gcacccgacg cctcactccc gtacccgatc gatcgtcacc gtcacgcctt cggcatccgg
     4681 cgccaccgga gcgctgacgc cctcgaggtc gccggggcgt gcggtggcat cgccaccctt
     4741 ggagacgcgc gccgcgatca cgacgcgctc gggcaccgcg gcgtcgccca tcatcagcct
     4801 cgcgccgtcg aagtcgaaat tcaccggcag ctcgctgccc ttgaagcgca gcgccgccag
     4861 cggggggccg ccggccgggc cacgcacgaa cacgaacacc gcgtcctcgg ccgcgacctg
     4921 gccggcgagc gcgccctcga cctgcaggcg gccggcgacc ttgagcggcg aggccggcgg
     4981 cggcgcggca ttgccttcgt tggtggcgag cggcgccatg ccggccttcg cgcgcgcctc
     5041 gttgatgctc gcgcgcacgc cgcgcgcgac gtcgtcgccc ggggggatca gggcgaggat
     5101 gcgctcccag tagccggcgg cggcggcgaa gtcgtcgcgc tggtaggcgg cggtgccagc
     5161 cagcgccagc gccttgggat gcttcggttc cgcctcgagc gcacgcagca ccagcgcctc
     5221 ggcatcgctc ccgaccttgc cggtctgcgc ggcgaccacg tcggcccagt cggagagcac
     5281 gtcggggtcg ttgggcacca gcttgtcgag gcgttcgtag gcggcggcag ccttggggaa
     5341 gtccttcagc accaggtagg tccgcgccag catcgcccag ccctcggcgt tgtcggggtt
     5401 ggcggcgagg tgcttctcca ggctgcccac catctcggtg acctgctcct gcgtgaagcc
     5461 ctgctggccc tcgacgttct tcgggtcgag cgcggcgggc tcgccgatca tcaggtaggt
     5521 gccgacggca agcgcgggga tggcgaggcc ggtggcgagc gcccacgcct tgctcggccg
     5581 gcggtcggcg ctgtcggccg tctcggcggc cacctcggcc tcctcgaggg cacggcgctc
     5641 gagctcctcg cggctcttcg catgggtcgc ggcgtcgatg cggccctcgg cgagctcggc
     5701 gtcgagatcg gcgagctgct cgcgcagcac ggtcagcacc atcgcgctct ggcgcatctg
     5761 cgcctggccg cggcggccgt agccgagcag cggcggcagg atcaatagca acgcgccggc
     5821 cacgagcagg ccggcgaaaa cgaggaaggc ggtcacttgc gggactccgg ggatgcggga
     5881 ttcgaggcgg cgtccccgcc gccggcgagc agcgcacgcg cgcgctcgcg gtctgcggcg
     5941 ctgagcgcgc cctcggcggc gatctcgctg cggcgccggc gcaggcgcca ggccagccag
     6001 ccagcgcccc ccagcagcag ggcgaacggc ccgacccaga gcagcccggt cacagccttg
     6061 aagggcggct cgtagagcac gaagtcgccg tagcgctcca cgaggtagtc gatcacgtcg
     6121 tccttgtcct tgcccgcggc gagctgttcg cgcacctggt tgcgcaggtc caccgcgagc
     6181 ggcgcgttgg attcggcgat cgactggttc tggcagacga ggcagcgcag cttgtgcgag
     6241 agctccatca tgtcggcttc gagcttggga tcggcggcga ccgccggggc gacgcccggg
     6301 tcgctcgcgg tggcgatgcc ggcgggggtg atggccgggc cgccttcggc cgtggcggca
     6361 tggagcgggg cggcgatcag cgcggcgagc gcgccgacgc cgagcacgcg acgcaggccg
     6421 tggagcccgg acgggacgag cttgggatca gccggcacga cgcagctcct cgaccttggg
     6481 catgatctgg gtctgcagcg actcgggagt gatcgggccg atgtgcttgt agcggatgcg
     6541 gccttcgcgg tcgatcagga aggtctcggg cacgccatac acgccgaagt cgatgccgac
     6601 gcggccgtcg atgtcgagca ccgagagcag gtaggggttg ccgtgatcgg cgagccagcg
     6661 ccgtgcgcgc tcgatcgcca tcgtggtctc ggcctcgagc gccatgccgc gggtgctgat
     6721 cgcgccgtcg ccgcgcactt ccttgtagtt gaggccgacc accggcacca gcttctgcgc
     6781 ggccatgcgt accagcaccg ggtgctcctg gcggcaggcc acgcaccacg aggcccacac
     6841 gttgagcagc cagacctggc cgcgcatctc ctcgagcgcg aaggtctgct cgggctggtc
     6901 gagacgggcg aggcggaagt tcggcgccgg cttgtcgatc agcggcgagg gcacctcgcg
     6961 cgggttgagg gtgaggccga aggcgaggaa gccggcgagg ccgaagaaca gcagcagcgg
     7021 tacgagaaac ttggctttca tggttttccc aggtcgaact caggcggcga cgcgactgcc
     7081 caccgccgcg gcggcgtcgc gcgcggccag gcggcgatag cggcggtcgg cggcggcgaa
     7141 cacgcctccg aggcccatca acacgcaacc tgcccagatc cagctgatga agggcttgta
     7201 gtagaggctg acgatccagg cgccgtcctc gagctgttcg ccgagcgaga cgtacacgtc
     7261 gcggaacacg ccgatgtcga gcgaggcctc ggtcatcggc atgccctggg cgagatagac
     7321 gcgcttctcg ggagtgagca tgtcgagcga gcggccgtcg cgcagcagtt cgatcctgcc
     7381 gcgcgcggcg tcgtagttgg ggccgtcggc atccaccgcg ccacggaaga cgaaagtgta
     7441 gccggcgagc tcggcgtgct ggccttcctt catcttgacg tcgaggtggc ggtccaggct
     7501 gcccaccatg gtgacgccga tgatgaagat gccgatgccg aagtgggcga gccacattcc
     7561 ccaccacgac gcggtgatcg aacgcaggcg cgaaccggcc gaggccccgg cgcgctcgcc
     7621 gaggcggcgg acgagcagtt gcaggctggc gagcaccacc caggccgcga gcgagaggcc
     7681 aagcgcggtg cgccaggtga ggtgatccac cgcccacgcg ctgccgacgc cgatcgccac
     7741 gctggcgacg aaggcgggcg ccacgcggcg cacctcggcg aggagctcgt cgtccttcca
     7801 gcgcaggaag gggccgaaca ccatcagcac cacgaccggg gtcatcagcg gcacgaacac
     7861 cgcctcgaag tagggcggcc cgaccgagat cttgccgagg ccgagggcgt cgaggaagag
     7921 cggatacagc gtgccgagca gcaccgaggc cgaggccacg gtgagcagga cgttattggc
     7981 gagcagggcg gtctcgcgcg acaccaggcc gaagctgccg ccgcccatga gcttgttcgc
     8041 acgcatcgcg tacagcagca gcgagaagcc gatcacgatc acgagcaggg cgaggatgta
     8101 gaggccgcgc ctggggtcgg tggcgaaggc gtgcacgctg gtgatgacgc ccgagcgcac
     8161 caggaaggtg ccgagcagcg acagcgagaa ggcgccgatc gccagcagca ccgtccagct
     8221 gcggaaggcg ccgcgcttct cggtgacggc gagcgagtgc atcagcgcgg tgccgagcag
     8281 ccagggcatg aaggaggcgt tctcgaccgg atcccagaac caccagccgc cccagccgag
//
LOCUS       DAOQNN010000112         8335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4321_length_8335_cov_9.432609, whole genome shotgun sequence.
ACCESSION   DAOQNN010000112 DAOQNN010000000
VERSION     DAOQNN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4321_length_8335_cov_9.432609"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PKV97_07815"
     CDS             <1..900
                     /locus_tag="PKV97_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HNB05821.1"
                     /translation="AIIDQPSQFDFYDGGGLDIAFLGLAQADREGNLNVSKFGPRLAG
                     AGGFINISQNAKKVVFVGTFSAGGLEVAVEDGCLRIVREGETRKFVDAVEHRTFSGTQ
                     AAKWRQDVLYVTERCVFRLCEDGLELIEIAPGIDLQRDILARMDFVPVMKGPPVRMDA
                     RIFADAPMGLRHDLLDVPLGRRLAYDAQQDVFFVDFEGLSVRTPQDIAATRAAVRAAL
                     APLGRKVDAVVNYDRFSIVPELVDDYVEMVKGLMDAHYHTVTRYTANGFLRMKLGEEL
                     EKRRIPAHFYASASEAWHGLETP"
     gene            complement(958..2004)
                     /locus_tag="PKV97_07820"
     CDS             complement(958..2004)
                     /locus_tag="PKV97_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNB05822.1"
                     /translation="MFRLPWPTRKDASTPVALALQGGGAHGAYTWGVLDRLLEAGVPI
                     EGISGTSAGAMNAVALAEGWTTGGAEGARAALDRFWTAVGDSVPFHLELLQSLSPSGD
                     GSLPSPMNMMLGLARVFSPYQLNPFELNPLRDVVRAQFDFERIRRACPLKLFIAATAV
                     RTGKVRLFRTAELDEAAVLASACLPTLHHAVEIDGEHYWDGGFTANPAIYPLLYECDT
                     PDLLMVLLNPLQHEQAPRSAEDIAARSMELGFSTTFLREMRMIAHARQFIAERPRWSP
                     IGRLERKLLRERFHLIDAPELGDAGSASKLDATASALLRLRELGRARAETWLQAHAHE
                     VGRRETIDVGALFL"
     gene            complement(2184..2564)
                     /locus_tag="PKV97_07825"
     CDS             complement(2184..2564)
                     /locus_tag="PKV97_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNB05823.1"
                     /translation="MTIRVRRAYDPPAAGDGCRVLVDRLWPRGLARENARIEHWLREL
                     APSNELRKWFAHDPTRWDEFRRRYADELRAPAAAAALAELHDLIAAHPVLTLLHAARD
                     TTHNNAEALRLWLACGAASRPGNA"
     gene            complement(2572..3549)
                     /gene="cysK"
                     /locus_tag="PKV97_07830"
     CDS             complement(2572..3549)
                     /gene="cysK"
                     /locus_tag="PKV97_07830"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNB05824.1"
                     /translation="MSTWYPDNAESIGRTPLVRLNRVLDGAQATVLAKIEGRNPAYSV
                     KCRIGAAMVKDAIAHGRLGPGQEIVEPTSGNTGIALAFVCAARGIPLTLTMPETMSVE
                     RRKLLVAYGARLVLTEGPKGMNGAIAKAKEIVDSAPGRYVLLQQFENPANPAIHETTT
                     GPEIWNDTDGGIDILVSGVGTGGTITGISRYIKRVRGKDIRSIAVEPAASPVISQTLA
                     GQPLTPAPHKIQGLGAGFVPKVLDLSLIDAVEQVSNEDAVLYARRLAREEGILAGISC
                     GAAVAAAARVAKQPENAGKTIVVILPDSGERYLSSILFEGLFDEKGMAI"
     gene            3723..6209
                     /locus_tag="PKV97_07835"
     CDS             3723..6209
                     /locus_tag="PKV97_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like protein"
                     /protein_id="HNB05825.1"
                     /translation="MKEKELRLALVLFGGVSLAVYQHGINRELLNLARASRAYHRVEG
                     PAAKQAPGHVYPAGAGADAWTAEVYFDLLKRLGRMVDLRVLVDVISGASAGAINGIAL
                     ARALAHDLSLAPVTRLWLERADMQRLIAPEARAGKWDKWYFRPLLRPLLAWARREGML
                     ETQPDPETLERALAFVRSRWFSAPLDGALLSAELLDGLLAMETGSVAAGSLLPSGTCL
                     SLAVTVTDFRGIERALFTHDPPLLREREHRHLLRFACEHRRTGELDSDFGLDNAPSLA
                     FAARASASYPGAFPPARLAEMDALLAARGLTWSTRERFLARNFAHYRASGMNPAEVVL
                     LDGSVLDNKPILAAVGYIRVHRAFREVDRRLIFIDPHAEARVGQGAGSDGGEGEPGWF
                     EVLRSALSDLPRHQPIHQELAEISRYNRQIRRLKATIVQSRPEVEALVERATGGALSG
                     PFTAVELRHWRLTSTNMLGAMPLVYDPWWRALVLEALDFLAGLLGSLCGCARESPGAR
                     WLQQVVEAWAAHAGILREHYRIADDVGEDADMPAFARVVIRFGIEYKRRRINFVLHEL
                     NTLYHALPGEGRCTTAPGVLDVVKTRIHECLDALAIYDDCAFVDAPAAAAVRALLQPA
                     TVAAAAQPEAEAVAFATRNALALDALIERIGDACRIGEANANMDAVLVSDAVRAIEPH
                     CRRQLLTAYLGYFYWDVILRPALGALALGSGPLEEVLIDRISPQDATALASVTEGRPA
                     LAGTAFAGFGGFLSAAARENDYLWGRLHAAERLVGIVASAVGGPGAPDAFELRGFRKR
                     AFEAILEEEASRLRAVPELIARLRAAVAAL"
     gene            6278..7549
                     /locus_tag="PKV97_07840"
     CDS             6278..7549
                     /locus_tag="PKV97_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB05826.1"
                     /translation="MSSPIAASSSLPAQLPLGLRDVAALGQVFTPEPVVRAMVALRRN
                     AGRVLEPSCGDGAFLRHLPGAVGLELDPDHCPPGAQAIDFFAYPERERFDAIIGNPPY
                     VRIQDIAESTRALIERGAYGDLLDGRANLYLFFIAKCLRHLRPGGELIFITPRDFLKA
                     TSAVKLNRLLVESGSITDAIELGDARVFDDAVPNCLIWRFEKGRSARAMRYCALGVGD
                     DLAAGLAAPAWEERHLVEAGGHLMFARGDYPLRLADVAFVKVGAVSGADELFTDAVNG
                     NRDFVCSSTVSTGQTRRMIWSEPGDPPPALLAPHKARLLQRKVTRFDESNWWMWGRLH
                     HRSAQPRVYVNGKTRVAKPFFVHSCTDYDGAVLAIFPRRADVDIEAFRDALNAVDWED
                     LGFVCDGRFLFTQRSLEHAPLPAAFEAFLPA"
     gene            7786..8094
                     /locus_tag="PKV97_07845"
     CDS             7786..8094
                     /locus_tag="PKV97_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNB05827.1"
                     /translation="MENDMATKIAANPKYQKLVATRSSFGWILTMIMMVVYYGYIAII
                     AFNKEVLAARLGEGVMTVGIPVGLGVIFFTILITGFYVRRANSEFDQLTREIVEESGK
                     "
     gene            8091..>8335
                     /gene="actP"
                     /locus_tag="PKV97_07850"
     CDS             8091..>8335
                     /gene="actP"
                     /locus_tag="PKV97_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361867.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNB05828.1"
                     /translation="MIGSNAKLLGGLALALLSSVALAAGGDLGQAEKQATNWTAIMMF
                     GVFVLGTLYITKWAASKTKSAADFYTAGGGITGFQNGL"
BASE COUNT         1156 a         2867 c         2981 g         1331 t
ORIGIN      
        1 gccatcatcg accagcccag ccagttcgac ttctacgacg gcggcgggct ggacatcgcc
       61 ttcctcggcc tcgcccaggc cgaccgcgag ggcaacctca acgtgtcgaa gttcggcccc
      121 cggctcgcgg gcgcgggcgg cttcatcaac atctcgcaga atgccaagaa ggtcgtcttc
      181 gtcggcacct tcagcgcggg cgggctggag gtggcggtgg aggatggatg cctgcgcatc
      241 gtccgcgagg gcgagacgag gaagttcgtc gacgcggtcg agcatcgcac cttcagcggg
      301 acgcaggcgg caaagtggcg gcaggacgtg ctctacgtca ccgagcgctg cgtgttccgg
      361 ctgtgcgagg acggactcga gctcatcgag atcgcgccgg ggatcgatct gcagcgcgac
      421 atcctcgctc gcatggactt cgtaccggtg atgaagggcc cgcccgtgcg gatggacgcg
      481 cgcatcttcg ccgacgcgcc gatgggcttg cgccacgacc tgctcgacgt tccgctcggg
      541 cgtcggctgg cctacgacgc gcagcaggac gtgttcttcg tcgacttcga ggggctgtcg
      601 gtgcgcacgc cgcaggacat cgccgcgact cgcgccgcgg tgcgcgccgc gctcgctcca
      661 ctcgggcgca aggtggatgc ggtggtcaac tacgaccgct tctcgatcgt gccggagctg
      721 gtggacgact acgtcgagat ggtgaaggga ctgatggatg cccattacca caccgtgacc
      781 cgctacaccg ccaacggctt cctgcgcatg aagctcggcg aggagctcga gaagcggcgc
      841 atccccgcgc acttctacgc gagtgcgagc gaagcctggc acgggctgga aaccccgtag
      901 cggggcgctg cgctgccgcg ctgcgcctgc gcggggcagg tgtcgggcat gcaggtctca
      961 caggaacagg gcgccgacat cgatcgtctc gcgccggccg acctcgtggg catgcgcctg
     1021 cagccaggtt tcggcgcgtg cgcggccgag ttcgcgcagc cggagcaggg cgctggcggt
     1081 ggcatcgagc ttgctcgcgc tgccggcgtc gccgagctcg ggggcatcga tcaggtggaa
     1141 gcgctcgcgc agcagcttgc gctcgaggcg gccgatcggc gaccagcgcg gacgctcggc
     1201 gatgaactgg cgggcgtggg cgatcatgcg catctcgcgc aggaaggtgg tggagaagcc
     1261 gagctccatg ctgcgtgccg cgatgtcttc ggcgctgcgg ggggcctgct cgtgctgcag
     1321 agggttcagc agcaccatca gcaggtccgg cgtgtcgcat tcgtagagca gcgggtagat
     1381 cgccgggttg gcggtgaaac cgccgtccca gtagtgctcg ccgtcgatct ccaccgcgtg
     1441 atgcagcgtg ggcaggcagg ccgaggccag cacggccgct tcgtcgagtt cggcggtgcg
     1501 gaagagcctg accttgccgg tgcgtaccgc ggtggcggcg atgaagagct tgagcgggca
     1561 cgcgcggcgg atgcgctcga agtcgaactg cgcacgcacc acgtcgcgca acggattgag
     1621 ctcgaagggg ttgagctgat agggagagaa cacgcgggcg agcccgagca tcatgttcat
     1681 cggcgagggg aggctgccat cacccgaggg gctgaggctt tgcagcagct cgaggtggaa
     1741 gggaacgctg tcgccgacgg ccgtccagaa gcggtcgagc gcggcgcgcg cgccctccgc
     1801 cccgcccgtg gtccagccct cggcgagcgc cacggcattc atcgcgccgg cgctggtgcc
     1861 gctgatgcct tcgatcggca ctcccgcctc gagcaggcgg tcgagcacgc cccaggtgta
     1921 cgcgccatgc gcgccaccgc cctgcagcgc gagcgcgacg ggggtggatg cgtccttcct
     1981 ggttggccac ggcaagcgaa acatcgaaac ccccttcggg tccgggcgga cggcagggga
     2041 gtcccttggc gcctgtccgg caagctgcag gtctgcattg tgcactgcac aatgcagatt
     2101 atcacgcagt ccggattaca gccgtgcgta ccggtaacag gatgcgcggg cgcgggcgtc
     2161 gtgcgcgccg gcccgccgcc gggtcaggca ttgccgggtc gggaagcggc tccgcacgcc
     2221 agccacaggc gcagcgcctc ggcgttgttg tgcgtggtgt cgcgcgcggc gtggagcagg
     2281 gtgaggacgg gatgcgcggc gatcaggtcg tgcaattcgg cgagtgccgc ggccgccgca
     2341 ggggcgcgca gctcgtcggc gtagcggcgg cggaattcgt cccagcgcgt cgggtcgtgg
     2401 gcgaaccact tgcgcagctc gttcgacggc gcgagctcgc gcagccagtg ctcgatccgt
     2461 gcgttctcgc gcgcgagtcc gcgcggccac aggcgatcga ccagcacccg gcagccgtcc
     2521 ccggctgcgg gtgggtcgta cgcgcggcgc acgcggatcg tcatggtctg gtcagatcgc
     2581 catgcccttc tcgtcgaaca ggccctcgaa gaggatcgag ctcaggtagc gctcgcccga
     2641 gtcgggcagg atcaccacga tggtcttgcc ggcgttctcg ggctgcttcg cgacgcgcgc
     2701 cgcggcggct accgccgcgc cgcaggagat gccggcgagg atgccctcct cgcgcgccag
     2761 gcggcgggcg tagagtaccg cgtcttcgtt gctcacctgc tcgaccgcgt cgatcagcga
     2821 gaggtcgagc actttcggca cgaagcccgc gcccaggccc tggatcttgt gcggcgcagg
     2881 ggtcagcggc tggccggcga gggtctggct gatcaccggg ctggcggcgg gctcgaccgc
     2941 gatcgagcgg atgtccttgc cgcgcacgcg cttgatgtag cgcgagatgc cggtgatggt
     3001 gccgccggtg cccacgcccg acaccaggat gtcgatgccg ccgtccgtgt cgttccagat
     3061 ctccgggccg gtggtggtct cgtggatcgc cgggttggcg gggttctcga actgctgcag
     3121 cagcacgtag cggccggggg cgctgtcgac gatctccttg gccttggcga tggcgccgtt
     3181 catgcccttg gggccttcgg tcagcaccag tctggcgcca taggcgacga gcagcttgcg
     3241 gcgctccacg ctcatggtct cgggcatggt cagggtcagc gggatgccgc gcgcggcgca
     3301 cacgaaggcg agcgcgatgc cggtgttgcc gctggtgggc tcgacgatct cctggccggg
     3361 gccgaggcgg ccgtgggcga tcgcgtcctt gaccatcgcc gcgccgatgc ggcacttgac
     3421 cgagtaggcc gggttgcggc cctcgatctt cgccagcacg gtggcctgcg cgccgtcgag
     3481 cacgcggttc aggcgcacga gcggggtgcg gccgatggat tctgcattgt cggggtacca
     3541 ggtggacatc ggttctcttc tcctcgttgg gggtgctccg gaagtatagg caaagcgcgc
     3601 cgcacggtcg cgccggatcg tcgcgcgtgg tggcggacgg cgcttgacgg cggcgcacgc
     3661 aggtgcaagc ttcaatccct tgcgcgaggt ccggcacgcg gtctggcacg tgggaggggg
     3721 cgatgaagga gaaggaactg cggctcgcac tggtgctgtt cggtggcgtt tcgctcgcgg
     3781 tgtaccagca cggcatcaac cgcgaactgc tcaacctcgc gcgcgcctcg cgcgcctacc
     3841 accgcgtcga agggcccgcc gccaagcagg cgcccggcca tgtgtatccg gcgggcgcgg
     3901 gcgccgacgc gtggaccgcc gaggtgtatt tcgacctgct caagcgcctc gggcgcatgg
     3961 tggacctgcg cgtgctggtc gacgtgatct cgggcgcgtc ggcgggggcc atcaacggca
     4021 tcgcgctcgc gcgcgcgctc gcgcacgatc tcagcctggc gccggtgacg cggctgtggc
     4081 tggagcgcgc cgacatgcag cgcctgatcg caccggaggc gcgcgccggg aagtgggaca
     4141 agtggtactt ccgcccgctg ctgcggccgc tgctggcgtg ggcccggcgc gagggcatgc
     4201 tcgagaccca gcccgacccc gagacgctcg agcgcgcgct cgccttcgtg cgttcgcgct
     4261 ggttcagcgc gccgctcgac ggggccttgc tgagcgcgga gctgctcgac ggcctgctcg
     4321 cgatggagac gggttcggtg gccgccggct cgctgctgcc ctcgggtacc tgcctgtcgc
     4381 tggcggtcac ggtgaccgac ttccgcggca tcgagcgcgc gctgttcacc cacgacccgc
     4441 cgctgctgcg cgagcgcgag catcgccacc tgctgcgctt cgcctgcgag catcgcagga
     4501 ccggcgagct cgacagcgac ttcggtctcg acaacgcgcc ctcgctcgcc ttcgcggcgc
     4561 gcgcctcggc gtcctatccg ggcgccttcc cgcccgcccg cctggccgag atggacgccc
     4621 tgctggccgc gcgcgggctg acgtggagta cgcgcgagcg cttcctggcg cgcaacttcg
     4681 cgcactaccg cgcgagcggc atgaacccgg cggaagtggt gttgctcgac ggcagcgtgc
     4741 tcgacaacaa gcccatcctc gccgcggtcg gctacatccg ggtccatcgc gccttccgcg
     4801 aggtggaccg gcggctgatc ttcatcgatc cgcatgccga ggcgcgcgtc ggccagggcg
     4861 ccggcagcga tggcggggag ggcgagccgg gctggttcga ggtcctgcgc agtgccctgt
     4921 ccgacctgcc gcgccaccag ccgatccacc aggagcttgc cgagatcagc cgctacaacc
     4981 gccagatccg ccgcctcaag gcgaccatcg tgcagagccg gcccgaggtc gaggcgctcg
     5041 tcgagcgcgc caccggtggt gccctgtcgg ggcccttcac ggccgtcgag ttgcgccatt
     5101 ggcggttgac ctcgaccaac atgctggggg cgatgccgct ggtgtacgac ccgtggtggc
     5161 gtgcgctggt gctggaagcg ctcgatttcc tcgccggcct gctcggcagc ctgtgcggct
     5221 gcgcacgcga atcgccgggc gcgcgctggc tgcagcaggt ggtggaggcg tgggcggcgc
     5281 acgccgggat cctgcgcgag cactaccgca tcgcggacga tgtgggcgag gatgccgaca
     5341 tgcccgcgtt cgcgcgcgtc gtgatccgtt tcggcatcga gtacaagcgc cgccgcatca
     5401 acttcgtgct gcacgagctc aacaccctct accacgccct gccgggcgag ggccgctgca
     5461 ccaccgcccc gggcgtgctc gacgtggtga agacgcgcat ccacgagtgc ctcgacgcat
     5521 tggcgatcta cgacgactgc gccttcgtcg atgcgcccgc cgccgccgcg gtgcgtgccc
     5581 tgctgcagcc ggcgaccgtc gccgccgctg cgcagcccga ggcggaggcg gtggcgttcg
     5641 cgacgcgcaa cgcgctcgcg ctcgacgcct tgatcgagcg catcggcgac gcctgccgga
     5701 tcggcgaggc caacgcgaac atggacgcgg tgctggtctc cgatgcggtg cgggcgatcg
     5761 agccgcactg ccgccgccag ttgttgaccg cctatctcgg ctacttctac tgggacgtga
     5821 tcctgcgccc cgcgctgggg gcgctcgcgc tcggcagcgg gccgctggag gaggtgctga
     5881 tcgatcgcat cagcccgcag gatgcgaccg cgctcgcctc cgtgacggag gggcgcccgg
     5941 cgctcgcggg caccgcgttc gcgggcttcg gcggcttcct cagcgcggcc gcgcgcgaaa
     6001 acgactacct gtgggggcgc ctgcatgcgg ccgagcgcct ggtcggcatc gtcgccagcg
     6061 ccgtcggcgg gcccggagcg cccgacgcgt tcgaactgcg tggattccgc aagcgtgcct
     6121 tcgaggccat cctcgaagag gaggcgagcc ggctgcgcgc cgttcccgag ctgatcgcgc
     6181 ggctgcgcgc agcggtcgcc gcgctctgag ggcgtgggcc gcgaagttcc gcgattgccg
     6241 gccctcgccg cgcgcgcgct tggtaagatc gcgccccatg tcctctccga tcgccgcctc
     6301 ttcctccctg cccgcccagc ttcccctggg cctgcgcgac gttgccgcgc tcggccaggt
     6361 gttcaccccc gagccggtgg tgcgcgccat ggtcgccctg cgtcgcaacg cgggccgcgt
     6421 gctcgagccc tcgtgcggcg acggcgcctt cctgcgccac ctgccgggcg cggtcggtct
     6481 cgagctcgac cccgaccact gcccgcccgg cgcgcaggcg atcgacttct tcgcctaccc
     6541 cgagcgcgag cgcttcgacg ccatcatcgg caacccgccc tacgtgcgca tccaggacat
     6601 cgccgagagc acgcgggcac tgatcgagcg cggcgcctac ggggacctcc tcgatggccg
     6661 cgccaacctc tacctcttct tcatcgccaa gtgcctgcgc cacctgcgcc cgggcggcga
     6721 gctgatcttc atcaccccgc gcgacttcct gaaggccacc tcggcggtga agctcaaccg
     6781 cctgctggtc gagtccggct cgatcaccga cgcgatcgag ctcggcgacg cgcgcgtgtt
     6841 cgacgacgcg gtgccgaact gcctgatctg gcgtttcgag aaggggcgca gcgcgcgcgc
     6901 gatgcgctac tgcgcgctcg gcgtcggtga cgacctcgcc gccgggctgg cggcgccggc
     6961 ctgggaggag cgccacttgg tcgaggccgg cggccacctg atgttcgcgc gcggcgacta
     7021 cccgctgcgc ctggccgacg tggcctttgt caaggtcggc gcggtgtcgg gtgcggacga
     7081 gctgttcacc gacgcggtga atggcaaccg cgacttcgtg tgctcgtcga cggtcagcac
     7141 cgggcagacc cggcgcatga tctggagcga gccgggcgat ccgccgccag cgctgctggc
     7201 gccgcacaag gcgcgcctgc tgcagcgcaa ggtcacccgc ttcgacgagt cgaactggtg
     7261 gatgtggggc cgcctgcacc accgcagcgc gcagccgcgg gtgtatgtga acggcaagac
     7321 gcgggtggcg aagcccttct tcgtccattc ctgcaccgac tacgacggcg ccgtgctggc
     7381 aatcttcccg cgccgcgccg acgtggacat cgaggccttc cgcgacgccc tcaacgcggt
     7441 ggactgggag gacctcggct tcgtctgcga cggccgcttc ctgttcaccc agcgcagcct
     7501 ggagcacgcg ccgctgccgg cggcgttcga ggcctttctg cccgcctgaa tcgggtgtcc
     7561 cccgtgtatg ccctccccgt gacgctcacg accggtccgg gtttccgaac gtcctgttca
     7621 tccgcatgga attgggagag aatgtgcaag ctttttgcaa gattttgtta agtatctgtt
     7681 ggaaaagaaa tttttatgcg ctcgtaagcg gagtgtaagt ctccgcccct aaggtgcgca
     7741 caaccgggca ggcgtccggt cctttcaccg cagaaggagg gagatatgga aaacgatatg
     7801 gcgacaaaaa tcgccgccaa ccccaagtac cagaaacttg tggccacgcg ctccagcttc
     7861 ggctggatcc tgaccatgat catgatggtc gtgtattacg gctacatcgc gatcatcgcc
     7921 ttcaacaagg aagtgctcgc cgcccgcctg ggcgagggcg tgatgacggt cggcatcccg
     7981 gtcggcctcg gggtgatctt cttcaccatc ctgatcaccg gcttctacgt gcgtcgcgcg
     8041 aactccgagt tcgaccagct gacccgcgaa atcgtcgagg agagcggcaa atgatcggta
     8101 gcaacgcgaa actcctgggc ggcctcgcgc tcgccctgct gtcctcggtg gcgcttgccg
     8161 caggcggcga cctcggccag gccgagaagc aggccaccaa ctggacggcc atcatgatgt
     8221 tcggcgtgtt cgtcctcggc acgctctaca tcaccaagtg ggcggcctcc aagaccaagt
     8281 cggcggccga cttctacacc gccggcggtg gcatcaccgg cttccagaac ggcct
//
LOCUS       DAOQNN010000113         8331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4327_length_8331_cov_11.196593, whole genome shotgun sequence.
ACCESSION   DAOQNN010000113 DAOQNN010000000
VERSION     DAOQNN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4327_length_8331_cov_11.196593"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKV97_07855"
     CDS             <1..468
                     /locus_tag="PKV97_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB05829.1"
                     /translation="VATLRHDPGRTALAQHFHTRRIEHLFHRFARIGRDFYAQEARWP
                     QAPFWAARRGWPDALPLHAKVRPETVRVARGPVVCAGRIVEQDVVVTPDQPLGVWHLD
                     GLAVAPMLAALRREGGDALEKAAGEALLCARFGLERARAAALLAWMRAQNWLD"
     gene            complement(570..1553)
                     /locus_tag="PKV97_07860"
     CDS             complement(570..1553)
                     /locus_tag="PKV97_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB05830.1"
                     /translation="MSPAAQPSGNAPYSYTLRHTLDADEHAACLTNWHQRYDQLTAGA
                     FDGVFEEFCFGRVQLFREGLNQSVHQAGGAWPGSRTFAVPVAIEGTGWFGGEMYDAHS
                     MLTLGGDDELDFRTPRRLEILACTADSAALHDYAHQVDHRNLEAELAGRKLAPTTPAG
                     IAALGQLLATMTASLRATPELLLHPQMRKAMEQALFATLLDTLSCGGGRAGAPSCRAR
                     QQVVARARAYMEAHIDEPITVADLCIELGISRRTLQYSFQDVLDLNPVKFLRAIRLNA
                     VRRSLKAADPNGRGTVADIAARWGFWHLSHFSAEYKTMFGELPSDTLRRAG"
     gene            2074..3657
                     /gene="peaA"
                     /locus_tag="PKV97_07865"
     CDS             2074..3657
                     /gene="peaA"
                     /locus_tag="PKV97_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009310 - amine catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     alpha"
                     /protein_id="HNB05831.1"
                     /translation="MKIRTLATAMGSMAALGVVLAAPPAHASAEALIRAKCLPCHTEN
                     EDGISRISHQRKTPEGWLMSVARMQIVHGLKITDEERRTVVKHLADRQGLAPSETTGV
                     RYAMERRLNTVEAFESEKFTQMCARCHSGARVMLQRRPAAEWEHLVHFHLGQFPTTEY
                     QALGRDRDWFGIALKEMVPELASKLPLQSEAWKQWQARAPQTVKGEWSLSGHLTGRGG
                     FSGVMKVTAGEGRDRYTLAFDGRWDDGRPMQGRGRALIYTGYEWRGDLVVDGTPMRQV
                     FAVEDGMMRGRMFLRDQDEVGADVVASLQQAGNSRVLAVHPAYLKAGEGAELRIVGSG
                     LAGEVTLPQGVKLVGTVKRSATEVVVRVQADAGARGVHPVAVGGASGGSLALYDSIAA
                     VKVSPAFAVARIGGNGTPTAKVEARFDAEAWAAGADGKAGTEDDFRIGVVPAKWSVEP
                     FDEAAVRDQDVEFAGLMDAASGVFVPGDAGPNPVRRMSASNVGNLKVVAEVAQGGERL
                     RGEGQVIVAPPRWNNPPLP"
     gene            3726..5156
                     /gene="peaB"
                     /locus_tag="PKV97_07870"
     CDS             3726..5156
                     /gene="peaB"
                     /locus_tag="PKV97_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase maturation
                     protein"
                     /protein_id="HNB05832.1"
                     /translation="MGAVLNLVQDNLHELRIDETRMLFHIPSSSLFALDPLSAALIDR
                     IRRQSLTVEALIDGLRTEFDADEVAEAIRELVALELIDDGRPAGAGTLAHTFERFPLT
                     TVVLNVNTGCNLSCTYCYKEDLDKPSAGRKMAFGTARDAIEMMLRESPDEPRYNVVFF
                     GGEPLSNLPLIKDVVAYCEARFAELGKQVDFVMTTNATLLADDTIDWLDAHRFGLSIS
                     IDGPKAIHDRNRLTVGGQGTYETVRRKAERLLARYHARPVGARVTLTHGTTEVERIWD
                     HLFNELGFAEVGFAPVTSGDISTFNLTGAELVEVFAGLKRLGARYLEAALEGRNIGFS
                     NMHQLITDLHEGHKKALPCGAGLKMLAVDHKGELNLCHRFTGSTLPTFGDVKNGIQRA
                     QLGDFLSQRLDRTDTGCASCRIRNLCSGGCYHESYARYGDPAHPTYHYCDLMRDWVDF
                     GIEVYSRIMAGNPAFIEQHISPRRAS"
     gene            5153..5479
                     /gene="qhpC"
                     /locus_tag="PKV97_07875"
     CDS             5153..5479
                     /gene="qhpC"
                     /locus_tag="PKV97_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     gamma"
                     /protein_id="HNB05833.1"
                     /translation="MKHLKAINNKARLLDKAVAEDRVEEVVAMSAIAGCAATVDPGWE
                     VDAFGGVSSLCQPMESDLYGCADPCWWPAQVPDVMNTYPDWNKDATNSREDWRNLGSV
                     FPGDKL"
     gene            5490..6626
                     /gene="peaD"
                     /locus_tag="PKV97_07880"
     CDS             5490..6626
                     /gene="peaD"
                     /locus_tag="PKV97_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009310 - amine catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     beta"
                     /protein_id="HNB05834.1"
                     /translation="MKKTMIGGALRRSAAALACLAAGVLPAAAANEARPLEPGSEYLM
                     VANYPNNLNVVDLASESVYKTCPLPDAFGPGTTQIAPDRRTAYILNNRFGTIYGIDLD
                     TCRLTFRAEMSQGDNERAKSIASFTVSADGKEVYAVQNPTTIHRDHYRVGAPRFAVYD
                     TRAGLEAKPVRVFPAPRQANIMQAGEDGSVYLAGPNLYKVDVNTGAMTVALPIRDWQR
                     STHAPLDVLYIWPVQTPTRDFTILYTTAKYQDDKQDMATAEYQYGYLNVNLKSGETEA
                     REFGPLTEIYFTGLRSPKDRNIVYGLLHRLTKYDIAQQKLVQAAELDHTYYTVLSNQA
                     GSRLYLTGTFNDISIHDADTLAKVGQVKLPGGDMSLGTGQVFVR"
     gene            6736..8052
                     /locus_tag="PKV97_07885"
     CDS             6736..8052
                     /locus_tag="PKV97_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNB05835.1"
                     /translation="MRMRITTAVLLAGLAALGTARAETLHERDGTTLSANIDGMAGAF
                     STREDYLGEGGRQWREGFIHGVLAGTTRAGGGELYGGLGAIAQGTWGDGDAGGYTDGR
                     ERDVDLEDAYVGWRSAGGAFDVSFGRQQFVLGDGFLIASDRINLGKGLDALGVDVDRG
                     GAYYLAAKKSFRNTAIVRIDPEGPWRGDAFWLQSGNPYQQDTELGGANLEYVSETHGT
                     LGLSWMKVLDVDQGAGLGIFDRRDGMRIASVRGQGSLGVENLFLAFEYVDQRGGSAAV
                     KNDADAWHVEGGWTFADLPWRPTLDYRHARFSADKSATARNEAFDPLFFGLSRGLGTW
                     FQGEVASNYAGPANSGNRVDRVELSLAPREDLSIGVQYWKFAPEGEAADLAGREFDLF
                     AFWQIDKHFTFVPLIGLYEPRGADVKALQGNDRRNVYLQAVLIFNY"
     gene            8106..>8331
                     /locus_tag="PKV97_07890"
     CDS             8106..>8331
                     /locus_tag="PKV97_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB05836.1"
                     /translation="MDRYKGELLSDDTRRFLAQPKRMAIGGEWVEALGGGMLEVVDPA
                     SGQVFDRVPAGEATDIDRAVEAARRAFEQGD"
BASE COUNT         1341 a         2795 c         2937 g         1258 t
ORIGIN      
        1 gtggctaccc tccgtcacga tccgggccgc accgcgctcg cgcagcactt ccatacgcgg
       61 cgtatcgagc acctgttcca ccgcttcgcc cgaatcgggc gcgatttcta cgctcaggaa
      121 gcgcgctggc cgcaggcgcc gttctgggcc gcgcgccgcg gctggccgga tgccctgccg
      181 ctgcacgcca aggtgaggcc ggagaccgtg cgtgttgcgc gcgggccggt ggtgtgcgcg
      241 gggcggatcg tcgagcaaga cgtcgtggtg acgcccgacc agccgctcgg cgtttggcac
      301 ctcgatggtc tggcggtggc gcccatgctt gccgccttgc gtcgagaggg aggcgatgcg
      361 ctggaaaaag ctgccggcga ggcgctgctg tgcgcgcgtt tcgggctcga gcgtgcgcgt
      421 gccgccgcct tgctggcctg gatgcgggca cagaactggc tggattgagc gccgcctgca
      481 tccatttcgt ctcccggacg gcggagcgtg ttgccgattt gggctaatgg ttccagcaaa
      541 agattttatt tttcgcccgc cgcgcctgct cagcccgcgc gcctgagcgt gtccgacggc
      601 agctcgccga acatcgtctt gtactcggcg gagaagtgcg acaggtgcca gaagccccag
      661 cgtgcggcga tgtcggcgac cgtgccgcga ccattggggt ccgcggcctt gagcgagcgg
      721 cgcaccgcat tcaggcggat cgcgcgcagg aacttgaccg ggttgagatc gagcacgtcc
      781 tggaagctgt actgcagggt gcggcgcgag atgccgagct cgatgcacag gtcggcaacc
      841 gtgatcggct cgtcgatgtg ggcctccatg taggcgcgcg cgcgcgccac cacctgctgg
      901 cgggcgcgac aggacggtgc accggcccgt ccgccgccac aggagagcgt gtcgagcagg
      961 gtcgcgaaca gcgcctgttc catcgccttg cgcatctgcg ggtgcagaag cagctcgggg
     1021 gttgcccgca ggctggccgt catggtcgcc agcagttgcc cgagcgccgc gattcccgcc
     1081 ggggtggtcg gtgccagctt gcgcccggcg agctcggcct cgaggttgcg gtggtccacc
     1141 tgatgcgcgt agtcgtggag cgcggccgag tcggcggtgc aggcgaggat ctcgagccgg
     1201 cgcggggtgc ggaagtcgag ctcgtcgtcg cctccgagtg tcagcatgga gtgcgcgtca
     1261 tacatctcgc cgccgaacca gcccgtgccc tcgatcgcga ccggcacggc gaaggtgcgc
     1321 gagccgggcc acgccccgcc agcctgatgc accgactggt tcaggccctc gcggaacagc
     1381 tgcaccctgc cgaagcagaa ctcctcgaac acgccgtcga aggcgcccgc ggtgagctgg
     1441 tcgtagcgtt gatgccagtt ggtcaggcag gcggcgtgct cgtcggcatc gagcgtgtgg
     1501 cgcagggtgt aggagtacgg ggcgtttccc gaaggctggg cagccgggct cattgcccgc
     1561 accccctggg cagttgccgg gtggcgttgg ccaacatgtc tcttcctctc tcgatcggtg
     1621 gccgatcgtg cttgtcgtta tgtttgtccc tgccgctcag ggtgcggatg aacgcttccc
     1681 cagtgctgtg cggccgtttt tcagaatggc acggcggcgg gtcgttttgc aacccggggt
     1741 ccgggaccgg ccgccgccgt ggcttgccgt tttcggataa cgcgcacgga aggtccgcgc
     1801 ctacgatctg ctcgccttcc acatcgaccc ggcgccgacc gggcgtgcgg aggcagaggc
     1861 agcagttgtg caggccctcg cagaagggca gcaagcggac tcgcgtgacc ttgcggtggc
     1921 gtgaggagga gacaggatcg gatcgcccgt cgcgagcggt gtccgtgctt gccctcggtc
     1981 tgtgcagcgc tgcggtgttc cccgcgcacg ggacggccat gccggcccac cgggtggacg
     2041 cctcatccaa ggacacgaag aggagatccg gtcttgaaaa tcagaaccct tgccactgcc
     2101 atgggcagta tggctgcgct cggcgtcgtg ctggccgccc ccccggcgca tgcctcggcc
     2161 gaagcgctga tccgcgccaa gtgcctgccc tgtcacaccg aaaacgagga cggcatcagc
     2221 cgcatcagcc accagcgcaa gaccccggaa ggctggctga tgtcggtggc acgcatgcag
     2281 atcgttcacg gcctgaagat caccgacgag gaacgccgca cggtggtcaa gcacctggcc
     2341 gaccgccagg gcctggcgcc gtccgagacc accggcgtgc gctacgcgat ggagcgtcgc
     2401 ctgaataccg tcgaggcctt cgagtcggag aagttcaccc agatgtgcgc gcgctgccat
     2461 tccggtgcgc gcgtgatgct gcagcgtcgc ccggcggcgg agtgggagca tctggtgcat
     2521 ttccacctcg gccagttccc gacgaccgag taccaggccc tcggccgcga ccgcgactgg
     2581 ttcggcatcg cgctgaagga aatggtgccg gagcttgcga gcaagctgcc gttgcagtcc
     2641 gaggcctgga agcagtggca ggcgcgcgcg ccgcagacgg tcaagggcga gtggagcctg
     2701 agcggccatc tgaccggccg cggcggattc tcgggcgtga tgaaggtgac ggcgggtgag
     2761 ggccgggacc gctacacgct cgccttcgac gggcgttggg acgatggcag gccgatgcag
     2821 ggcagggggc gggcgctgat ctacaccggt tacgagtggc gcggcgacct ggtcgtcgac
     2881 ggcacgccga tgcgccaggt gttcgcggtc gaggacggca tgatgcgcgg ccgcatgttc
     2941 ctgcgcgacc aggacgaggt cggcgccgac gtcgtcgcca gcctgcaaca ggcgggcaac
     3001 agccgcgtgc tggcggtgca tccggcctac ctcaaggcgg gtgaaggcgc cgaactgcgc
     3061 atcgtcggct ccgggctggc gggcgaggtg acgctgccgc agggcgtgaa gctggtcggt
     3121 acggtcaagc gttcggcgac cgaggtggtc gtccgcgtgc aggccgatgc cggggcgcgc
     3181 ggcgtgcatc cggtcgccgt ggggggggct tcgggcggca gcctcgcgct gtacgacagc
     3241 atcgccgcgg tcaaggtgtc gcccgcgttc gcggtcgcac gcatcggcgg caacggcacg
     3301 cccacggcca aggtcgaggc gcgcttcgac gccgaggcct gggcagccgg cgcggacggc
     3361 aaggctggca ccgaggacga cttccgcatc ggcgtggtgc cggcgaagtg gtcggtcgag
     3421 cccttcgacg aggcggcggt gcgcgatcag gacgtcgagt tcgccggcct gatggatgcc
     3481 gcgagcgggg tcttcgtgcc gggtgatgcc ggtcccaacc cggtgcggcg gatgtcggcg
     3541 agcaacgtgg gcaacctgaa ggtggtggcc gaggtggcgc agggtggcga acgcctgcgc
     3601 ggcgaaggcc aggtgatcgt ggcgccgccg cgctggaaca acccgccgct gccctgatcg
     3661 gtggcgcgtc ctgttccggc aggcgcggcc tgccctccgc aatcccgacc ccgaaggagg
     3721 ctcccatggg tgccgtcttg aatctggtgc aggacaacct gcacgagctg cgcatcgatg
     3781 agacgcgcat gctgttccac atcccgagca gttcgctgtt cgcgctcgac ccgctgagcg
     3841 cggcgctgat cgatcgcatc cgccgtcaga gcctcaccgt cgaggccctg atcgatggcc
     3901 tgcgtaccga gttcgacgcc gacgaggtgg ccgaggcgat ccgcgagctg gtcgcgctcg
     3961 agctcatcga cgacggccgc cccgccggcg ccggcacgct cgcgcacacc ttcgagcgct
     4021 tcccgctcac cacggtggtg ctcaacgtca acaccggctg caacctgagc tgtacctact
     4081 gctacaagga agacctcgac aagccctcgg cgggccgcaa gatggccttc gggaccgcgc
     4141 gcgacgcgat cgagatgatg ttgcgcgagt cgccggacga gccgcgctac aacgtcgtct
     4201 tcttcggcgg cgagccgctc agcaacctgc cgctgatcaa ggacgtggtc gcgtactgcg
     4261 aggcgcgctt cgccgagctc ggcaagcagg tcgatttcgt catgacgacg aacgcgaccc
     4321 tgctcgccga cgacaccatc gactggctcg atgcccaccg cttcgggctg tcgatcagca
     4381 tcgacggtcc gaaggcgatc cacgaccgca accggctcac cgtcggcggc cagggcacct
     4441 acgagaccgt gcggcgcaag gccgagcgcc tgctggcgcg ctaccacgcg cggccggtgg
     4501 gggcacgggt cacgctcacc cacggcacca ccgaggtcga gcgcatctgg gaccacctgt
     4561 tcaacgagct gggctttgcc gaagtgggct tcgcgccggt gacctcgggc gacatcagca
     4621 ccttcaacct cacgggcgcg gagctggtcg aggtcttcgc cgggctgaag cggctcggtg
     4681 cgcgctatct ggaggcggcg ctggagggac gcaacatcgg tttctccaac atgcaccagc
     4741 tgatcaccga cctgcacgaa ggccacaaga aggcgctgcc ctgtggcgcc gggttgaaga
     4801 tgctcgcggt cgaccacaag ggcgaactga acctgtgcca tcgcttcacc ggctccacgc
     4861 tgccgacctt cggcgacgtg aagaacggta tccagcgcgc gcagctcggc gatttcctgt
     4921 cccagcgcct ggatcgcacg gataccggct gcgcgagctg ccgcatccgc aacctgtgct
     4981 cgggcggctg ctaccacgag agctacgcgc gctacggcga tcccgcacat cccacctacc
     5041 actactgcga tctgatgcgc gactgggtgg acttcggcat cgaggtctac agccgcatca
     5101 tggccgggaa cccggccttc atcgaacagc atatttcccc gaggagggcg tcatgaaaca
     5161 tctcaaggcg atcaacaaca aggcgcggct gctggacaag gcggtcgcgg aggaccgggt
     5221 cgaggaggtg gtggcgatga gcgccatcgc cggctgcgcg gcgacggtgg acccgggctg
     5281 ggaagtggac gccttcggcg gtgtgtcctc gctgtgccag ccgatggaat ccgatctcta
     5341 cggctgcgcc gatccgtgct ggtggccggc ccaggtgccg gacgtgatga acacctaccc
     5401 ggactggaac aaggacgcga ccaactcgcg cgaggactgg cgcaatctcg gctcggtctt
     5461 cccgggcgac aagctgtaag ggggcggaca tgaagaagac gatgatcggc ggcgcactgc
     5521 gccgcagcgc ggccgcgctc gcctgcctgg ccgccggagt cctgcccgcc gcagcggcaa
     5581 acgaggcgcg gccgctcgag ccgggcagcg aatacctgat ggtggccaac tacccgaaca
     5641 acctgaacgt ggtcgatctg gccagcgaga gcgtctacaa gacctgcccg ctgcccgacg
     5701 ccttcggacc cggcaccacg cagatcgcgc ccgaccgccg caccgcctac atcctcaaca
     5761 accgtttcgg cacgatctac ggcatcgatc tcgacacctg caggctcacc ttccgcgccg
     5821 agatgtcgca gggcgacaac gagcgtgcca agtcgatcgc atccttcacc gtcagcgcgg
     5881 acggcaagga ggtctatgcg gtgcagaacc cgaccaccat ccatcgcgac cactatcgcg
     5941 tgggggcgcc gcgcttcgcg gtctacgata cccgtgccgg tctggaggcc aagcctgtgc
     6001 gtgtgtttcc ggcgccgcgc caggccaaca tcatgcaggc gggtgaggac ggctcggtct
     6061 acctggccgg accgaacctc tacaaggtgg atgtgaacac cggtgcgatg accgtcgccc
     6121 tgccgatccg cgactggcag cgttccacgc acgcgccgct cgatgtgctg tacatctggc
     6181 cggtgcagac gccgacgcgg gacttcacca tcctctacac cacggcgaag taccaggacg
     6241 acaagcagga catggcgacc gccgagtacc agtacggcta tctgaacgtg aacctgaaga
     6301 gcggcgagac cgaggcacgc gagttcggac cgctgaccga gatctacttc accgggctgc
     6361 gttcgccgaa ggaccgcaac atcgtctacg gcctgctgca ccgcctgacc aagtacgaca
     6421 tcgcgcagca gaagctggtg caggccgccg agctcgacca cacctactac accgtgctga
     6481 gcaaccaggc cggcagccgg ctctacctca ccggcacctt caacgacatc tccatccacg
     6541 acgccgacac gctggccaag gtcgggcagg tgaagctgcc gggcggggac atgtcgctcg
     6601 gtaccgggca ggtcttcgtg cgctgagcgc ccgcttccgg acggggcaag cccgcagcag
     6661 cgggacacca cggcgcgcca agcggcgcac cgcggtgtcc cgcgcgccaa aacaatcatc
     6721 gaggagacga acatcatgcg catgcgcatc accacggccg tcctgctggc cggactggcg
     6781 gcgctcggca cggcccgggc agagaccttg cacgaacgcg acggcaccac gctgagcgcg
     6841 aatatcgacg gcatggccgg cgccttctcc acgcgcgagg actatctcgg cgagggcggc
     6901 aggcagtggc gcgagggctt catccatggc gtgctggcgg gcaccacccg tgccggcggc
     6961 ggcgagctgt acggcggcct gggcgcgatc gcacaaggca cctggggcga cggcgatgcc
     7021 ggcggctata ccgacggccg cgagcgcgac gtcgacctgg aggatgcgta cgtcggctgg
     7081 cgcagcgcag gtggcgcgtt cgacgtctcg ttcggccgcc agcagttcgt gctcggcgac
     7141 ggcttcctga tcgcgagcga ccggatcaac ctcggcaagg ggctcgatgc gctcggtgtg
     7201 gacgtggatc ggggcggggc ctactacctc gccgccaaga agagcttccg caacaccgcg
     7261 atcgtgcgca tcgatcccga ggggccctgg cgcggcgacg ccttctggct gcagtcgggc
     7321 aacccctacc agcaggacac cgagctcggc ggcgcgaacc tggagtacgt gtcggagacg
     7381 catggcacgc tcgggctgag ctggatgaag gtgctcgacg tcgaccaggg cgccgggctg
     7441 ggcatcttcg accgtcgcga cggcatgcgc atcgccagcg tacgcggcca gggcagcctc
     7501 ggcgtggaga acctgttcct cgccttcgag tacgtcgacc agcgcggcgg cagcgccgcg
     7561 gtgaagaacg acgccgacgc ctggcacgtc gagggcgggt ggaccttcgc cgacctgccc
     7621 tggcgcccca cgctcgacta ccgccacgca cgcttctccg ccgacaagtc cgccaccgcg
     7681 cgcaacgagg ccttcgaccc gctgttcttc ggcctcagcc gcggcctggg gacctggttc
     7741 cagggtgagg tcgcgtccaa ctacgccggc ccggccaaca gcggcaatcg ggtggaccgc
     7801 gtggagctga gcctggcacc ccgcgaggat ctctccatcg gcgtgcagta ctggaaattc
     7861 gcgcccgagg gcgaggccgc ggacctggcc gggcgcgagt tcgacctgtt cgcgttctgg
     7921 cagatcgaca agcacttcac cttcgtgccc ctgatcggcc tctacgagcc gcgcggtgcg
     7981 gacgtgaagg cgctgcaggg caacgaccgc cgcaatgtct atctgcaggc ggtgctgatt
     8041 ttcaactatt gagggggcgg cctgcggccg tcccgggcaa cacacatcaa acacggagac
     8101 gaggaatgga ccgatacaag ggtgaactgc tgagcgacga cacgcgccgg ttcctggcgc
     8161 agcccaagcg catggcgatc ggcggcgaat gggtggaggc cctcggcggt ggcatgctcg
     8221 aggtggtgga tcccgccagc gggcaggtgt tcgatcgtgt gccggcgggc gaggcgaccg
     8281 acatcgaccg cgcggtggag gcggcgcggc gcgccttcga gcagggcgac t
//
LOCUS       DAOQNN010000114         8260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4391_length_8260_cov_8.774284, whole genome shotgun sequence.
ACCESSION   DAOQNN010000114 DAOQNN010000000
VERSION     DAOQNN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4391_length_8260_cov_8.774284"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1100)
                     /locus_tag="PKV97_07895"
     CDS             complement(132..1100)
                     /locus_tag="PKV97_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNB05837.1"
                     /translation="MEKIVFLDRATLAPQIRLRRPSFAHDYAEHARTAPAEVVERLAG
                     ATIAILNKVRLDAAAIAQLPALRLVAVAATGTDCVDKAACAARGIAVCNIRGYARDTV
                     PEHVFALLLALRRQLIPYRDDVLAGEWQRAGQFCFFNHPIHDLAGTRLGIVGAGDLGQ
                     RVAAIARAFGMEPVFAARKGERPGDDSYLAWDEVLATSDVISLHCPLTEATRGLVGLP
                     EFRAMARRPLLINTARGGIVDEHALVQALDEGLISGAGFDVTDGEPPAPDSPLMRIAA
                     RPNVLLTPHIAWASDEAQQALADQLVDVIEKFVAGQPKNLVSGGFA"
     assembly_gap    1364..1463
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1631..3421)
                     /locus_tag="PKV97_07900"
     CDS             complement(1631..3421)
                     /locus_tag="PKV97_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB05838.1"
                     /translation="MNPPSKRATPPTHAGRFAEPFAPPPPRNPLDRRVHAAIARATAS
                     VSPIALLLATVDWAGHLAVSPGKRMELADLGIAQMRRLLRYAQQLAIAPAGSAAHECI
                     EPPAQDRRFQAPEWHAWPFNLMHQSFLLGQEWWDAATHGVAGVSRHHEQVVAFAARQL
                     LDMFSPGNFLPTNPVVLRQTLASGGMNLARGAVHAVEDFERLVGGAPPAGAEDFVVGR
                     DVAVTPGKVVLRNRLIELIQYAPATADVYAEPVLIVPAWIMKYYILDLSPHNSLVRYL
                     VERGHTVFCISWKNPGTDERELDMDDYLQLGFFAALDAINAIVPGQKVHATGYCLGGT
                     LLAIAAAAMDRDGDARLGSMTLFTAQTDFTEPGELALFIDDSEVSLLEAQMEETGFLT
                     AGQMAGAFQILRSNDLLWSRVVGEYLMGERTPMNDLMAWNADATRMPARMHSQYLRRL
                     FLNDDLSEGRYPVGGKPVALSDIELPVFCVATLTDHVAPWRSVYKLHYLVPTEISFVL
                     TSGGHNAGIVSEPGRPRRSFRMHTRPAGGNYVPPDDWLERTPEQEGSWWPAWSDWLAA
                     RSGTRVAPPAIGAPDYPALDDAPGCYVHEK"
     gene            complement(3448..6942)
                     /locus_tag="PKV97_07905"
     CDS             complement(3448..6942)
                     /locus_tag="PKV97_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB05839.1"
                     /translation="MINVVSRDRLRNLADFLLPVLLMAAILSATSLLGHVRLKDATAD
                     IERETHLRLEQIAEVDEIGDLLGRIHAELHRIVMPGVAPGPDPAAQALAALWLDNQLA
                     ALESRFDAIGVWSTAQASHAQAKASLQSYRTLVLPLLATDAREPRAAALDLADADRLH
                     NALVRHTEELAIAVTADAAEKNADAIHIIQRRLDQSLSTSLTVIVLTLLLAFTLAKRL
                     ANDRNILADALRRMAQDQLEAEHYEALTRLAAKKGDRLGELAQALLGFRETRLAQKDD
                     ETLMKAVIEQAPCAIELVDPVTLRFLRANAYSRQALGYTEAELRQRSVMDIQAELQPA
                     QLEALARDIVARRSANFETVHRASDGRLLEARVHIRVIRLHGQDYLLGIWQDVSAEHQ
                     ALQALKKFSMAIEQSPNAVLITDTEARIEYVNDAFLHTSGYTRAEVIGRNPRLLKSDK
                     TPAEVYAQLWDALVKGEPWRGELVNRRKDGGEYIEQAQISPIRQPDGRITHYLAIKQD
                     ITEKRRMLEELERHRHHLEQLVAERSAALHTALDAAEVANRAKSEFLANMSHEIRTPL
                     NAIIGLGHLLARDLREDEQKARLEKIITAARHLLQIINDILDLSKIEAGRLDLEANDF
                     DPGAMLEGTLELVREQAEQKGLQLSLDLDMLPSRVRGDALRLGQIVLNFASNAVKFTP
                     HGSVAIRGRVLSTAGEQSIVRFEVADTGIGVDASQCERMFQPFEQADPSTTRKYGGTG
                     LGLAISRRLTELMGGRIGVDSTLGKGSTFWVEVPLQLRPASAGRQPSAAAGAPPCAEH
                     DRLCGRRILLAEDNPVNREVAVAILRGTGAVIDTAENGQEAVDMASTTRYDLILMDMQ
                     MPVMDGITATRLLRSRDELRKLPILAMTANAFAEDRQRCIDAGMNDHITKPIEPQALC
                     EKIWRWLPPDAPAADLPTPPAVQAGEEPVDDDPLAPLAGLHELDVATGLQYVLDDREI
                     YLGLLRLFAESDYGKLLQESIDAGDAVALRRAAHSIKGGAATIGATALSLAAAELEAR
                     VVSGLADEAQSMQAGPLHEAASALAARTTRFCADLEAATREAAADAASAPERSDEDCV
                     EAAPARIAEIVATVEHQLTLGDIAVRELLEAHRGLLRNVLGERFAHFEERIGNFDFET
                     ALDDLRAADAARKDTQAR"
     gene            complement(6962..8092)
                     /locus_tag="PKV97_07910"
     CDS             complement(6962..8092)
                     /locus_tag="PKV97_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB05840.1"
                     /translation="MPADSSTRSTVLIVDDTPENLTILGETLRPHYRVRAAISGERAL
                     HAVTIGALPDVILLDVMMPDMDGYQVLQALQADAATRDIPVIFVTAMDSVEGETHGLA
                     LGAVDYITKPFNPDIVLARVRTHIELAKARERLHDENAWLEREVARRTNENALIRDLS
                     LSALACLAEVRDIETGHHILRTQSFVELLAHHLAGHPDYREALAGERLEIIVRSAPLH
                     DIGKVGIPDSILLKPGRLTSAEFEIMKKHPVIGADAITRAMAQAANSRSGDGALPGGA
                     FAFMETAREIALSHHEKWDGSGYPAGLQGRQIPVSARLMALADVFDALSSRRVYKEPL
                     APEDVTRIIVAERGRHFDPVIVDAFLELRPAFAEVAQRFAEA"
BASE COUNT         1120 a         2591 c         3086 g         1363 t
ORIGIN      
        1 ctcctacgtc ccacaccggg cgttgcgcct acgttccctg gcggttctgt gggagcgacg
       61 caagtcgcga tcgcagcgcg cggggtccgc gccgcgattg atcgatcgca ggatcgcgac
      121 tcgcgtcgcg cctacgcaaa cccgccggag acgagattct tcggctggcc agcaacaaat
      181 ttttcgatga catcgaccag ctgatcggcc agcgcctgct gcgcttcatc cgaggcccag
      241 gcgatgtgcg gggtgaggag gacgttcggg cgggcggcga tgcgcatcag cgggctgtcg
      301 ggcgcgggcg gttcgccgtc ggtgacgtcg aagccggcgc cggagatcag gccctcgtcg
      361 agcgcctgca cgagggcgtg ctcatccacg atgccgccgc gcgcggtgtt gatcagcagg
      421 ggacgccgcg ccatcgcgcg gaattcgggc aggccgacga ggccgcgggt ggcctcggtg
      481 agcggacagt gcaggctgat gacgtcgctg gtcgcgagca cctcgtccca ggccaggtag
      541 ctgtcgtcgc cggggcgttc gcccttgcgc gcggcgaaca ccggctccat gccgaaggcg
      601 cgcgcgatcg ccgccacgcg ctggccgagg tcgccggcgc cgacgatgcc gagccgggta
      661 ccggcgaggt cgtggatggg gtggttgaag aagcagaact gcccggcgcg ctgccattcg
      721 ccggcgagca cgtcgtcgcg gtaggggatg agctggcggc gcagcgcgag caggagcgcg
      781 aagacgtgct cgggcacggt gtcgcgcgcg tagccgcgga tgttgcacac cgcgatcccg
      841 cgcgccgcgc aggcggcctt gtcgacgcag tcggtgccgg tggcggcgac cgcgaccagg
      901 cgcagcgcgg gcagttgcgc gatcgccgcg gcgtcgaggc ggaccttgtt gaggatcgcg
      961 atggtggcgc cggcgaggcg ttcgacgacc tcggcggggg cggtgcgcgc gtgttcggcg
     1021 tagtcgtggg cgaaggacgg acggcgcagg cggatctgcg gcgcgagcgt ggcgcggtcg
     1081 aggaagacga tcttttccat ggggggctct gtgggggaac gaagcggatc gccggggcgg
     1141 gcgatgggga ggggggcggt gcggagaggt ggaatcgcga cttgcgtcgc tcccacagga
     1201 agaccgcggc gctccgacgg caagaacgcg gcgctcgcgc agcaggaccg cggcgctcgg
     1261 ggtggctgtg ggagcgacgc aagtcgcgat caaggcggca gcgctgcgct gggcgcaggc
     1321 ggatcgaggc gggcgggaat cgcgacttgc gtcgctccca cacnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnccgctcc cacacccgct cccacacccg ctcccacacc
     1501 cgctcccaca cccgctccca cacccgctcc tgcacccgct cctgcagccc gccgcgggcg
     1561 tagcggggtg tgcggggcgc ggcgggggcg gttgcatcgg cggcgcggtc aagctgcccg
     1621 catcgtccgt tcacttctca tgcacgtagc agcccggcgc gtcgtcgagc gcgggatagt
     1681 ctggggcacc gatcgccggc ggcgccacgc gcgtgccgct gcgcgcggcg agccagtcgc
     1741 tccaggccgg ccaccacgag ccttcctgct cgggcgtgcg ctcgagccag tcgtccggcg
     1801 ggacgtagtt gccgcccgcg gggcgggtgt gcatgcggaa gctgcgccgc gggcggccgg
     1861 gctcgctgac gatgccggcg ttgtggccgc cgctggtgag cacgaagctg atctcggtcg
     1921 gcaccaggta gtgcagcttg tacaccgagc gccagggcgc gacgtggtcg gtgagggtgg
     1981 cgacgcagaa caccggcagc tcgatgtcgg acagcgccac cggcttgccg cccaccgggt
     2041 agcgcccctc gctgaggtcg tcgttgagga agagccggcg caggtactgg ctgtgcatgc
     2101 gcgcgggcat gcgcgtggcg tcggcgttcc aggccatgag gtcgttcatc ggcgtgcgct
     2161 cgcccatgag gtattcgccg acgacgcgcg accacagcag gtcgttggag cgcaggatct
     2221 ggaacgcgcc cgccatctgg ccggcggtga ggaagccggt ctcctccatc tgcgcctcga
     2281 gcaggctgac ctcgctgtcg tcgatgaaga gcgcgagctc gcccggttcg gtgaagtcgg
     2341 tctgcgcggt gaacagcgtc atcgagccga ggcgcgcatc gccgtcgcgg tccatcgctg
     2401 ccgcggcgat cgccagcagg gtgccgccga ggcagtagcc ggttgcgtgc accttctgcc
     2461 cggggacgat ggcattgatc gcgtcgagcg cggcgaagaa gccgagctgc aggtagtcgt
     2521 ccatgtcgag ctcgcgctcg tccgtccccg ggttcttcca cgagatgcag aacacggtgt
     2581 ggccgcgctc gaccaggtag cgcaccagcg agttgtgcgg cgagaggtcg aggatgtagt
     2641 acttcatgat ccaggccggc acgatcagca ccggctcggc gtacacgtcg gcggtggcgg
     2701 gcgcgtactg gatgagctcg atgaggcggt tgcgcagcac caccttgccc ggggtgaccg
     2761 cgacgtcgcg gccgacgacg aagtcctcgg cgccggccgg cggggcgccg ccgacgaggc
     2821 gctcgaagtc ctcgacggcg tggacggcgc cgcgcgcgag gttcatgccg ccgctcgcca
     2881 gggtctggcg cagcactacc gggttggtgg gcaggaagtt gcccggcgag aacatgtcga
     2941 gcagctggcg cgcggcgaag gcgacgacct gctcgtgatg gcgcgagacg ccggccacgc
     3001 cgtgggtggc ggcgtcccac cattcctggc cgagcaggaa ggactggtgc atcaggttga
     3061 agggccaggc gtgccactcg ggcgcctgga agcggcggtc ctgcgcgggc ggctcgatgc
     3121 actcgtgcgc ggcgctgccc gccggggcga tggcgagctg ctgtgcgtag cgcagcaggc
     3181 ggcgcatctg cgcgatgccg aggtcggcga gctccatgcg cttgcccggc gagaccgcga
     3241 ggtggccggc ccagtccacc gtcgcgagca gcagcgcgat cggcgacacc gaggcggtgg
     3301 cgcgcgcgat cgccgcgtgc acccggcgat cgagcgggtt gcgcggcggt ggcggggcga
     3361 agggctcggc gaagcgaccg gcgtgagtgg gcggggtcgc gcgtttgctg ggcgggttca
     3421 tcctgtactt gcctccatgc atccggttca gcgggcttgc gtgtccttgc gggctgcgtc
     3481 ggccgcccgc aggtcgtcga gggcggtttc gaaatcgaag ttgccgatgc gctcctcgaa
     3541 gtgcgcgaag cgctcgccga gaacgtttcg cagcagcccg cggtgggctt cgagcagttc
     3601 gcgcaccgcg atgtcgccga gggtgagctg gtgctcgacg gtggcgacga tctccgcgat
     3661 ccgtgccggc gccgcttcga cgcagtcctc gtcggatcgc tccggagcgg acgctgcgtc
     3721 ggctgcggct tcgcgtgtcg ccgcctccag gtctgcgcag aaacgggtgg tgcgagcggc
     3781 cagcgcgctc gcggcttcat gcagcggacc ggcctgcatg gattgtgcct cgtccgccag
     3841 gccactgacc acccgggctt ccagctcggc ggcggcaagg ctcagtgcgg tcgcgccgat
     3901 cgtggccgca ccgcccttga tcgagtgggc ggcgcggcgc agggcgacgg catcacccgc
     3961 gtcgatcgat tcctgcagga gcttgccgta gtcgctctcc gcgaacaggc gcaacagccc
     4021 gaggtagatc tcccggtcgt cgaggacgta ctgcagacct gtcgcgacat cgagctcgtg
     4081 gagccccgcg aggggggcga ggggatcgtc atccacgggt tcctcgcccg cctggactgc
     4141 cggcggggtg ggcaggtcgg cggccggggc gtcgggggga agccagcgcc agatcttctc
     4201 gcacagggcc tgcggctcga tcggcttggt gatgtggtcg ttcatccccg cgtcgatgca
     4261 gcgctggcga tcctctgcga aggcgttggc ggtcatcgcc aggatgggca gcttgcgcag
     4321 ctcgtcgcgg ctgcgcagga ggcgggtggc ggtgatgccg tccatgaccg gcatctgcat
     4381 gtccatgagg atgaggtcgt agcgggtggt gcttgccatg tcgaccgcct cctgtccgtt
     4441 ctccgcggta tcgatcaccg ctccggtccc acgcaggatg gcgacggcga cctcgcggtt
     4501 gacggggttg tcttcggcga ggaggatgcg ccgcccgcac agccggtcgt gttcggcgca
     4561 ggggggggcg ccggcggctg cgctcggttg ccggcccgcg ctggcggggc gcaactgcag
     4621 cggcacctcg acccagaagg tgctgccctt gcccagcgtg ctgtcgacgc cgatcctgcc
     4681 gcccatgagt tcggtcaggc ggcgcgagat cgccaggccg aggccggtgc cgccgtactt
     4741 gcgtgtggtg gacggatcgg cctgctcgaa aggctggaac atgcgctcgc actggctcgc
     4801 atccacgccg atgccggtgt cggcgacctc gaagcgcacg atcgactgct cgcccgcggt
     4861 ggacagcacg cggccgcgga tcgccacgct cccgtgtggc gtgaacttca ccgcgttgct
     4921 ggcgaagttg agcacgatct ggccgaggcg cagcgcgtcg ccgcgcaccc tggagggcag
     4981 catgtcgagg tcgagcgaga gctgcaggcc cttctgctcg gcttgctcgc gcaccagttc
     5041 cagcgtgccc tcgagcatcg ccccggggtc gaagtcattc gcttcgaggt ccagccgccc
     5101 ggcctcgatc ttcgacaggt cgaggatgtc gttgatgatc tgcagcaggt gccgggccgc
     5161 ggtgatgatc ttctccagcc gggccttctg ttcgtcctcg cgcagatccc tggcgagcag
     5221 gtggccgagt ccgatgatcg cgttcagcgg cgtgcggatc tcgtggctca tgttggcgag
     5281 gaactcgctc ttggcgcggt tggcgacctc ggcggcgtcg agtgcggtgt gaagcgcggc
     5341 ggagcgttcg gcgacgagct gttcgaggtg gtggcggtgg cgctccagct cctcgagcat
     5401 gcggcgcttt tcggtgatgt cctgcttgat cgcgaggtag tgcgtgatgc gcccgtcggg
     5461 ctggcggatg ggcgagatct gcgcctgctc gatgtattcg ccaccgtcct tgcgccggtt
     5521 gaccagctcg ccgcgccagg gctctccctt gacgagagcg tcccagagtt gggcatacac
     5581 ctccgcgggg gtcttgtccg acttgagcag gcgcgggttg cgaccgatca cctctgcgcg
     5641 cgtgtagccg ctggtgtgca ggaaggcatc attcacgtat tcgatgcggg cctcggtgtc
     5701 ggtgatgagg accgcgttgg ggctctgctc gatcgccatg gagaatttct tcaaggcctg
     5761 caaggcctgg tgttcggccg acacgtcctg ccagatgccg agcaggtagt cctggccgtg
     5821 caggcggatc acccggatgt gcaccctggc ctcgagcagc ctgccgtcgg atgcgcggtg
     5881 gaccgtctcg aaattcgcgc tgcgccgggc aacgatgtcc cgggccaggg cctcgagctg
     5941 ggccggctgg agctcggcct ggatgtccat gacgctgcgc tggcgcagct cggcttcggt
     6001 gtagccgagt gcctgtcgcg agtaggcgtt ggcccggaga aagcgcagcg tcaccgggtc
     6061 gaccagctcg atcgcgcatg gcgcctgctc gatcaccgcc ttcatcaggg tctcgtcgtc
     6121 cttctgggcg aggcgggttt cacggaaacc gagcagggcc tgggccagtt cgccgagacg
     6181 gtcgcctttt ttcgccgcca gtcgggtgag cgcctcgtag tgttccgctt cgagttggtc
     6241 ctgcgccatc ctgcgcagcg catcggccag tatgttgcgg tcattggcga gccgcttcgc
     6301 cagggtgaag gcgagcagga gcgtaagcac gatcacggtc aggctcgtgc tcaggctctg
     6361 gtcgagccgg cgctggatga tgtggatcgc gtcggcgttc ttctcggctg cgtcggccgt
     6421 caccgcaatc gccagttctt cggtgtggcg gacgagcgcg ttgtggagcc gatcggcgtc
     6481 ggcgaggtcg agtgcggcgg cccgcggctc gcgtgcatcg gttgcgagca ggggcagcac
     6541 gagggtccga taggattgca ggctcgcctt ggcttgcgca tgcgacgctt gggcggtcga
     6601 ccagacgccg atcgcatcga agcggctctc gagtgcggcg agctggttgt cgagccacag
     6661 tgcagcaagc gcctgcgcgg ccggatccgg gccaggggcc acgcccggca tgacgatgcg
     6721 gtgcagttcg gcatggatgc gaccaagcaa gtcgccgatt tcgtccacct cggcgatctg
     6781 ctcgagacgg agatgggttt cgcgctcgat gtccgcggtg gcgtccttca ggcgtacgtg
     6841 gcccagaagg ctggtcgcgc tcaggatcgc cgccatgagc aggaccggca gcaggaagtc
     6901 ggcgaggttg cgcagccgat cgcgcgacac gacgttgatc atgggcgagg ctcccggccg
     6961 ctcaggcctc ggcgaagcgt tgtgcgacct cggcgaaggc ggggcgcaat tcaaggaatg
     7021 cgtccacgat gaccgggtcg aagtggcggc cgcgttcggc gacgatgatc cgggtcacgt
     7081 cctccggcgc caacggttcc ttgtaaacgc gccgcgagct cagtgcgtcg aagacgtcgg
     7141 cgagcgccat gaggcgcgcg gaaacgggaa tctggcggcc ttgcaggcct gcggggtagc
     7201 cgctgccatc ccatttctcg tgatggctga gcgcgatctc gcgtgcggtt tccatgaacg
     7261 cgaaagcgcc ccccggcaag gccccgtctc cgctccggct gtttgccgcc tgcgccatgg
     7321 cgcgggtgat cgcatccgcg ccgatcaccg gatgtttctt catgatctcg aattctgcgg
     7381 acgtcaggcg ccccggcttg aggaggatcg agtccgggat gccgaccttg ccgatgtcgt
     7441 gcaggggggc gctgcgcacg atgatctcga ggcgctcgcc tgcgagcgcc tcgcggtagt
     7501 cggggtgccc ggccagatgg tgggcgagaa gctcgacgaa ggactgcgtg cgcaggatgt
     7561 ggtggccggt ttcgatgtcg cgcacttcgg caaggcaggc gagcgcggag aggctgaggt
     7621 cgcggatcag cgcgttctcg ttcgtgcggc gggcgacttc gcgttcgagc catgcgttct
     7681 cgtcgtgcag gcgctcgcgc gccttggcaa gctcgatgtg ggtgcgtacc cgtgcaagca
     7741 cgatgtcggg attgaacggc ttggtgatgt aatccaccgc gccgagggcg aggccgtgcg
     7801 tctcgccctc caccgagtcc atcgcggtga cgaagatgac cgggatgtcg cgcgtcgccg
     7861 cgtcggcctg gagggcctgc agcacctggt agccgtccat gtccggcatc atgacgtcga
     7921 gcaggatcac gtcgggcagc gcgccgatgg tgaccgcatg cagtgcgcgc tctcccgaga
     7981 tggcggcgcg gacacgatag tgcgggcgca gcgtctcgcc gaggatggtg aggttctcgg
     8041 gcgtgtcatc gacgatgagg acggtgcttc gtgtgctgct gtcggcgggc atggcctgga
     8101 ttccgcgggt taggcgtggc cgattgtact cgggtcgggg agtcgggtat ctgtgcctaa
     8161 atatcaataa agaagaaatc gtcacattca attttatcga aaattccttt cggggtgccg
     8221 cggcttagtt ctcggtcgag cgcgatcgac cgccccggcg
//
LOCUS       DAOQNN010000115         8230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4417_length_8230_cov_9.412232, whole genome shotgun sequence.
ACCESSION   DAOQNN010000115 DAOQNN010000000
VERSION     DAOQNN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4417_length_8230_cov_9.412232"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PKV97_07915"
     CDS             <1..936
                     /locus_tag="PKV97_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNB05841.1"
                     /translation="ATTLPADSNPRSRRLAAELAAGARDHAEILERVLARLRALRLAY
                     TLRPPLLGRHAADEFLFDARRGFCEHFASAFAVLMRAAGVPTRIVTGYQGGEINPIDG
                     QLVVRQSDAHAWTEVWLQGRGWLRVDPTALAAPERIDGGLAAALADAGELPLMLRADM
                     AWLRGLRHRWEAVANLWNQHVLGYNPERQRELLARIGLGADRLAQTLGVLAGAAVLLF
                     AALYAWSLRATRVRDPLARTWERFCAKMAAAGAARPAWQGPQDYAEELATHFPAHAME
                     LRGICMLYARLRYGPPAPEQQLRILHNRIASLRLK"
     gene            933..2015
                     /gene="mltB"
                     /locus_tag="PKV97_07920"
     CDS             933..2015
                     /gene="mltB"
                     /locus_tag="PKV97_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HNB05842.1"
                     /translation="MIPTPSLPARPSSPTQRPPYRQRKLGGILAAFAIALCTPALAHA
                     SYAEREDARVFAAEIASRHALDAGEIERALAGARHDPEVIRLITPPSRKGVRSWRSYR
                     ARFLDRTRIDGGMRFWHEHADTLERAERETGVPAEVIVAIIGVETIYGRNTGNFETLS
                     TLATLAFDYPPRAELFRRELEALFLLAREQGRDPASYYGSFAGALGYPQFLPSSMRAY
                     AVDFDRNGAIDFDGDPVDAIGSVANYLRAHGWQAGAPVAERARLSPATDAAGLVAAGI
                     EPTLAPALLADNGITTADGRPVAAAATLVDLETPGADTEYWLGYRNFYVITRYNRSSF
                     YAMSVFELAEALRLGRLVEEIRAERR"
     gene            2027..2812
                     /locus_tag="PKV97_07925"
     CDS             2027..2812
                     /locus_tag="PKV97_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HNB05843.1"
                     /translation="MPASAPPSSSDPSSLRPAPLGLADLGGALRFGITQFRAIPLLSM
                     AFALLFVVIGALLFGILEFGAIAPMSLSLAGGFMLVGPVLLAGFFGVSDKLRKGRQPS
                     FGDIWSGFRQMPRGGWVVSFVCGLLFLIWITDAGILYGFMVGREPIGFVRLLRIEQVV
                     MDYAGYSAIMGGVLAFILFAVSAFSIPLIYDGRATLVSGVVASVRAVFGRFGVMMCWA
                     FLLAAVIMGSVMALPLLLVSLPVMAYASRELYFRTFPPAAPAT"
     gene            complement(2847..3785)
                     /gene="rpoS"
                     /locus_tag="PKV97_07930"
     CDS             complement(2847..3785)
                     /gene="rpoS"
                     /locus_tag="PKV97_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HNB05844.1"
                     /translation="MEEPVNLDELESQQEPDLPPEVEVFSFQAPPVVENEFFSDVTQL
                     YLNEIGANPLLTAEEELVIARRVRMGDFDARQTMIERNLRLVVNIAKHYLNRGIPLLD
                     LVEEGNLGLMHALEKFDPERGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHVVKELN
                     QVLRAQRHIEADCNGESSLEQIANRLGKTIEEVRSILALGEHTASLDAPLDIDPSLSI
                     GESLADEQHISADLRIQCSEVEQLVREWLAILNDKQRMVIRHRYGIDECELLTLEELA
                     ERLELTRERVRQIQLEALGQLRRILRRRGISRDALL"
     gene            complement(3797..4621)
                     /locus_tag="PKV97_07935"
     CDS             complement(3797..4621)
                     /locus_tag="PKV97_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB05845.1"
                     /translation="MHKNTSHLIALVAAALLAGCASQGTAPVRDGSGVSPAPAAAVAP
                     ASGYHTVRPGDTLLGIARQYGVTLPDLVAWNRITDPDQIHVGQSLRVSPSASTGAIAG
                     AGGAIATPIPSTGSATVPTVREPVGGRAPVTEPAAVESPPPAAAEAPPSTMSGQWRWP
                     ASGSVIAGFNEASNKGLDIGGAVGEPVYAAAAGKVVYAGSGLRGYGKLIVIKHNQEYN
                     SVYAHNDKLLVKEDDQVAQGQKIAELGSSEADRPKLHFEIRKQGKAVDPTGYLPAR"
     gene            complement(4638..5291)
                     /locus_tag="PKV97_07940"
     CDS             complement(4638..5291)
                     /locus_tag="PKV97_07940"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNB05846.1"
                     /translation="MSVRASDPGQASRARARMVERLRAQGIRDERVLAAMQAVPRHAF
                     VDEGLAFSAYDDTPLPIGFQQTISQPFVVARMLELLRNGRELGRTLEVGAGCGYQAAV
                     LSLLATEVYAVERIRGLLDRARANLRPLRLPNVRLKLADGSLGLPEAAPFDSIVVAAA
                     AVGLPPALKEQLASGGRLIIPVGGAEQRLLLIERQGNTFRETWCEAVRFVPLIAGTD"
     gene            complement(5288..6031)
                     /gene="surE"
                     /locus_tag="PKV97_07945"
     CDS             complement(5288..6031)
                     /gene="surE"
                     /locus_tag="PKV97_07945"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNB05847.1"
                     /translation="MRILVSNDDGYFAPGIAALAEALGTIGQVTVVAPERDRSGASNS
                     LTLDRPLSLRQTSNGFYHVNGTPTDCVHLAVTGMLDHLPDMVVSGVNHGANMGDDTVY
                     SGTVAAATEGFLLGVPSIAVSLVSKSATDFTAAARVARDLAERFMRNPFPRPVLLNVN
                     VPDVAYERLQGIRVTRLGKRHKAEPVVRSETPRGETVYWVGAAGGAADAGEGTDFHAV
                     ANGFVSVTPLQIDLTHTGLIAPVFDWLQR"
     gene            complement(6183..6259)
                     /locus_tag="PKV97_07950"
     tRNA            complement(6183..6259)
                     /locus_tag="PKV97_07950"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6223..6225),aa:Pro,seq:ggg)
     gene            complement(6344..6724)
                     /locus_tag="PKV97_07955"
     CDS             complement(6344..6724)
                     /locus_tag="PKV97_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNB05848.1"
                     /translation="MQASSPETGGEQLPPIPAKRYFTIGEVSELCCVKPHVLRYWEQE
                     FTQLKPVKRGGNRRYYQHHEVMLIRRIRQLLYQEGFTISGARNRLGESAIQEQENGVE
                     LEQCRSTLRQLRAEIAATLQELRA"
     gene            complement(6705..7010)
                     /locus_tag="PKV97_07960"
     CDS             complement(6705..7010)
                     /locus_tag="PKV97_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNB05849.1"
                     /translation="MTLTKAELADLLFEQVGLNKREAKDMVEGFFEEIRTALERGDSV
                     KLSGFGNFQLRDKPQRPGRNPKTGEEIPITARRVVTFHASQKLKAAVEQLSDASKQP"
     gene            complement(7012..>8230)
                     /locus_tag="PKV97_07965"
     CDS             complement(7012..>8230)
                     /locus_tag="PKV97_07965"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNB05850.1"
                     /translation="VEAVATDALPARAPVRLRPARARRVLGIALVDAEMLALLRRVHL
                     QVECVGDDFLVTPPSWRFDIEIEEDLIEEIARLYGYDNIPAVAPQGSLMMLPSTENGR
                     PVWALRHLLAARGYQEVVNFAFVEEAWERDFCANENPIRLANPIASHLSVMRSSLIPG
                     LVGTLAANRKRQIDRVRVFEVARVFERKADGEPVRGFHQVMNIAALAAGQVVPEQWGE
                     RARSVDFYDVKGDLEALFAPRELQFAPLSHPALHPGRAAAVRLDGHMIGVLGELHPVW
                     VQRYELGTAPVVFEVALDAALAARVPAYAEISRMQAVRRDLALVVGNEVPVERVLEVL
                     REAAPALVREIALFDLYQGKGIDHDKKSLAFKVSMQDTRRTLEDAEVDAAIALLVTHA
                     ETVLGARLRGAGA"
BASE COUNT         1241 a         2892 c         2767 g         1330 t
ORIGIN      
        1 gccaccaccc tgcccgccga cagcaacccg cgcagccgcc gcctcgccgc cgaactcgcc
       61 gctggcgcgc gcgaccacgc cgagatcctc gagcgcgtgc tcgcccgact gcgcgccctg
      121 cgcctggcct acacgctgcg cccgccgctg ctcggccgcc acgcggcaga cgagttcctc
      181 ttcgacgccc ggcgcggctt ctgcgagcac ttcgcctccg ccttcgccgt gctgatgcgc
      241 gccgcaggcg tgccgacccg gatcgtgacc ggctatcagg gaggcgagat caacccgatc
      301 gacggtcagc tggtggtgcg ccagtccgac gcccacgcct ggaccgaggt ctggctgcag
      361 ggacgcggct ggctgcgggt ggacccgacc gcgctcgccg cccccgagcg catcgacggc
      421 gggctggccg cggcgctggc cgacgcgggc gagttgccgc tgatgttgcg cgccgacatg
      481 gcctggctgc gcggcctgcg ccaccgctgg gaagctgtcg cgaacctgtg gaaccagcat
      541 gtccttggct acaatcccga gcgccagcgc gaactcctcg cgcgcatcgg cctcggcgcg
      601 gacagactgg cgcagaccct gggcgtgctc gccggcgcag cggtactgct gttcgccgcc
      661 ctctatgcct ggagcctgcg cgccacgcgc gtgcgcgacc cgctcgcgcg cacctgggag
      721 cgcttctgcg cgaagatggc cgccgctggc gcggcccgcc cagcctggca ggggccgcag
      781 gactatgccg aagaactggc cacgcacttc ccggcgcatg ccatggaact acgcggcatc
      841 tgcatgctct acgcccgcct gcgctatgga ccgcccgccc cggagcagca actccggatt
      901 ctgcacaacc gcatcgcctc actgcgcctg aaatgatccc gaccccgtcc ctccccgccc
      961 gcccttcctc gcccacgcag cggccgccgt accggcagcg caagctcggc gggatcctcg
     1021 ccgccttcgc gatcgcgctg tgcacgcccg cgctcgcgca tgcgtcctac gccgagcgcg
     1081 aggacgcgcg cgtcttcgcc gcggaaatcg ccagccgcca tgcactcgac gccggcgaga
     1141 tcgagcgcgc gctcgccggc gcccgccacg atccggaggt gatccgcctg atcacgcccc
     1201 cctcgcgcaa gggcgtgcgt tcctggcggt cctatcgcgc gcgtttcctc gaccgcaccc
     1261 gcatcgacgg cgggatgcgt ttctggcatg agcacgccga taccctggaa cgcgccgagc
     1321 gcgagaccgg cgtccccgcc gaggtcatcg tcgcgatcat cggggtggag acgatctacg
     1381 ggcgcaacac cggcaacttc gagaccctct ccacgctcgc cacgctggcc ttcgactacc
     1441 cgccgcgcgc cgaactcttc cgccgcgaac tcgaggccct gttcctgctc gcgcgcgaac
     1501 agggacgcga cccggcgagc tattacggct ccttcgccgg cgcgctcggc tacccgcagt
     1561 tcctgccgag cagcatgcgc gcctatgcgg tggacttcga ccgcaacggc gccatcgact
     1621 tcgacggtga cccggtggac gcgatcggca gcgtggccaa ctacctgcgc gcgcatggct
     1681 ggcaggcagg cgcaccggtc gcagagcgcg ctcgcctgtc gcccgcgacc gatgctgccg
     1741 gcctggtggc ggccggcatc gagccgacgc tcgcgcccgc gctgctcgcc gacaacggca
     1801 tcaccaccgc cgacggccgc cctgtcgccg ccgcggcgac cctggtggac ctggagacgc
     1861 caggcgccga caccgagtac tggctcggct accggaactt ctacgtcatc acgcgctaca
     1921 atcgaagcag tttctacgcg atgtcggtgt tcgagctcgc cgaggcgctg cgcctgggcc
     1981 ggctggtgga ggagatccgc gccgaacgcc gctgaaggat ccgcccatgc ccgcaagcgc
     2041 cccgccgtcg tcctccgacc cctcctccct ccgtccggcg ccgctcggcc tcgccgacct
     2101 cggcggcgcg ctgcgtttcg gcatcacgca gttccgggcc attccgctcc tgagcatggc
     2161 cttcgcacta ctcttcgtgg tgatcggagc cctgctcttc ggcattctcg agttcggcgc
     2221 gatcgcgccg atgagcctgt cgctcgccgg gggcttcatg ctcgtgggtc cggtactgct
     2281 ggccggcttc ttcggtgtct ccgacaagct gcgcaagggc cgccagccga gcttcggcga
     2341 catctggagc ggttttcggc agatgccgcg cggcggctgg gtggtgtcct tcgtttgcgg
     2401 gctgctgttc ctgatctgga tcaccgacgc cggcatcctc tacggcttca tggtgggccg
     2461 cgagccgatc ggcttcgtgc gcctgctgcg aatcgagcag gtcgtgatgg actacgccgg
     2521 ctacagcgcg atcatgggtg gagtgctggc cttcatcctg ttcgccgtgt cggcgttctc
     2581 gatcccgctg atctacgatg gccgcgcgac gctggtgtcg ggcgtggtgg cgagcgtgcg
     2641 tgcggtgttc ggtcgcttcg gcgtgatgat gtgctgggcc ttcctgctcg ccgcggtgat
     2701 catgggcagc gtcatggccc tgccgctgct gctggtcagc ctgccggtga tggcctacgc
     2761 gagccgggaa ctctacttcc gcacctttcc ccctgccgcg cccgcgacat gacgggactc
     2821 ctgcgcgcac ccgcggttgt gcttgcctag agcagcgcgt cgcgcgagat tccgcgccgg
     2881 cgcaggatcc ggcgcagctg gcccagcgcc tcgagctgga tctggcgcac gcgctcgcgg
     2941 gtgagttcga ggcgttcggc gagttcttcg agcgtgagca actcgcactc gtcgatgcca
     3001 tagcggtgac gaatcaccat gcgctgcttg tcgttgagga tcgcgagcca ttcgcgcacc
     3061 agttgttcga cttccgagca ctgtatgcga aggtcggcgg agatgtgctg ctcgtccgcg
     3121 agcgactcgc cgatcgacaa ggaggggtcg atgtcgagcg gcgcgtcgag cgaggcggtg
     3181 tgctcgccga gcgcgaggat gctgcgcacc tcctcgatcg tcttgcctag ccgattggcg
     3241 atctgctcga gcgaggactc gccgttgcaa tcggcctcga tgtggcgctg cgcgcgcagg
     3301 acctgattga gttccttcac cacgtggacg ggcaggcgga tcgtgcgcga ctggttcatg
     3361 atcgcacgct cgatgttctg ccgaatccac cacgtcgcgt aagtcgagaa gcggaagccg
     3421 cgctcggggt cgaacttctc gagcgcgtgc atgaggccga ggttgccctc ctcgaccaga
     3481 tcgagcaagg gaatcccgcg attgaggtag tgcttggcga tattgacgac caggcgcagg
     3541 ttgcgctcga tcatcgtctg ccgggcatcg aagtcgccca tccgcacgcg gcgggcgatc
     3601 accagctcct cctcggcggt cagcagcgga ttggcaccga tctcgttgag gtagagctgg
     3661 gtgacgtcgc tgaagaactc gttctcgaca accggcggcg cctggaacga gaacacctcc
     3721 acctcagggg gaagatccgg ttcctgctga ctttccagtt catcaagatt gaccggttct
     3781 tccatcctcg ccctcctcag cgcgcaggca gatacccggt cggatcgacc gccttgccct
     3841 gcttgcggat ctcgaaatga agcttgggcc gatcggcttc cgaactgccg agctcggcga
     3901 tcttctggcc ctgtgcgacc tggtcgtctt ccttgaccag gagcttgtcg ttatgagcat
     3961 acacggagtt gtactcctga ttatgcttga tcacgatcaa tttgccataa ccccgcaggc
     4021 cgctgcctgc gtagaccacc ttgccggccg ccgccgcata caccggctcg ccgaccgcgc
     4081 caccgatgtc gagccccttg ttggaagcct cgttgaagcc ggcgatcacg ctgccggagg
     4141 ccggccagcg ccattggccg ctcatcgtcg aaggcggcgc ctccgccgcg gcgggcggcg
     4201 gactctcgac cgcagcgggc tcggtcaccg gcgcacggcc gccgaccggt tcccgcacgg
     4261 tcgggaccgt agcggagccg gtcgaaggga tcggcgtcgc gatggccccc ccagcccccg
     4321 cgatcgcgcc cgtcgaggcc gacggcgaca cgcgcaggct ctggccgacg tggatctggt
     4381 cggggtcggt gatccggttc cacgccacca gatcgggcag ggtgacgcca tattggcgtg
     4441 cgatgccgag cagggtgtcg cccggacgga cggtgtgata acccgaggcc ggcgcgaccg
     4501 cagcggccgg tgcgggcgaa accccgctac cgtcgcgcac cggcgccgtc ccctgcgagg
     4561 cgcagccggc aagcagcgcg gcggcgacga gggcgatgag atgagaagtg ttcttgtgca
     4621 tccgaaaata ccgtgattca gtcagtaccc gcgatcaggg gaacgaagcg caccgcctcg
     4681 caccaggtct cgcggaaggt attgccctgg cgctcgatca gcaacaggcg ctgctccgct
     4741 ccaccgaccg gaatgatcag ccgcccgccc gaggcgagct gctccttcaa tgccggcggc
     4801 agcccgaccg ccgccgcggc gacgacgatg ctgtcgaagg gagcggcctc ggggagacca
     4861 agactaccgt cggcaagttt cagccgcacg ttgggcaggc gcaaggggcg caggttcgcg
     4921 cgcgcgcgat cgagcaggcc gcggatgcgc tctacggcat agacctcggt ggcgagcagc
     4981 gacagcaccg ccgcctgata accgcagccc gcgccgacct cgagcgtgcg cccgagctcg
     5041 cgcccgttgc gcagcagttc gagcatgcgc gccaccacga agggctgcga gatggtctgc
     5101 tggaagccga tcggcagcgg cgtgtcgtcg taggcgctga atgccagccc ctcgtccacg
     5161 aaggcgtggc gaggcaccgc ctgcatcgcc gcgagcacgc gctcgtcgcg gatgccttgg
     5221 gcgcgcagcc gctcgaccat gcgcgcgcgt gcgcgcgagg cctgccccgg gtcggatgcg
     5281 cgcacgctca ccgctgcaac cagtcgaaga ccggagcgat caggccggta tgggtgaggt
     5341 cgatctgcag cggcgtgacc gacacgaagc cgttggccac cgcatggaag tcggtgcctt
     5401 ctcccgcgtc ggcggcaccg cccgcggcac cgacccaata gaccgtctcg ccgcgcggcg
     5461 tctcgctgcg caccaccggc tcggccttgt ggcgcttgcc caggcgggtg acgcggatgc
     5521 cttgcaggcg ctcgtaggcg acatcgggca cgttgacgtt gagcagcacc ggacgcggaa
     5581 aggggttgcg catgaagcgc tcggcgaggt cgcgagcgac gcgtgcggcg gcggtgaaat
     5641 cggtggccga cttgctcacc agtgagaccg cgatcgaggg cacgccaagc agaaagccct
     5701 cggtggcggc cgcgaccgtg cccgaataca ccgtgtcgtc ccccatgttg gcgccgtgat
     5761 tgacaccgga gacaaccatg tcgggaaggt ggtcgagcat cccggtgacc gcgagatgga
     5821 cgcagtcggt gggggtgcca ttgacgtgat agaagccgtt ggaggtctgg cgaagcgaga
     5881 gcggacggtc gagcgtcagc gagttgctcg cgccgctgcg gtcgcgctcg ggagcgacca
     5941 cggtcacctg gccgatcgtg ccgagcgcct cggcgagcgc cgcgatgccg ggggcgaagt
     6001 aaccgtcgtc gttgctgacc aggatgcgca tcttgattcc gtgcgggtgg ctgcgaggga
     6061 ttgcaagggc ctgcaagggg cgccgcgaac cggcgggagc ggagatatta gcatggcgta
     6121 ccaggccgca cgcctggcga acggcacaaa caaaaaaacc ggcctgggcc ggttagtttg
     6181 attggtcggg gcggcgggat tcgaactcgc gaccccttgc accccatgca agtgcgctac
     6241 caggctgcgc tacgccccga cacaagccgc cattatagca gcggttttca gaaataccag
     6301 tacttttgct gcgcgatgat cagcccgcga atcgtgccgc ggatcaggcg cgcagttcct
     6361 gcagcgtggc cgcaatctcg gcgcgcagtt gccgcagcgt ggacctgcat tgctcgagtt
     6421 ccacgccgtt ctcctgctcc tggatcgccg actccccaag gcggttgcgc gcaccggaga
     6481 tggtgaaccc ctcctgatac agcagctggc ggatgcgccg gatcagcatg acctcgtggt
     6541 gctggtaata gcgccggttg ccaccgcgct tgaccggttt cagctgggtg aactcctgct
     6601 cccagtagcg cagcacgtgc ggcttcacgc agcacagctc gctcacctcg ccgatcgtga
     6661 aatagcgctt cgcaggaatc ggcggcagtt gctcgccacc ggtttcaggg ctgcttgctt
     6721 gcatcgctga gttgctccac cgcggccttc agtttctggc tggcatggaa ggtgaccacg
     6781 cgccgggcgg tgatcgggat ttcctcgccg gtcttggggt tgcgacccgg acgctgcggc
     6841 ttgtcgcgca actggaagtt gccgaagccg gagagcttga cgctgtcgcc gcgctccagc
     6901 gccgtccgga tctcctcgaa gaagccctcc accatgtcct tggcttcacg cttgttgagc
     6961 ccgacctgct cgaacagcag gtcggcgagc tcggccttgg tgagggtcat gtcaggctcc
     7021 cgcgcctcgc aggcgcgcac ccagcacggt ttcggcatgc gtcacgagca gcgcgatggc
     7081 cgcatccact tcggcgtctt cgagggtgcg tcgagtatct tgcatcgata ccttgaaggc
     7141 aaggctcttc ttgtcatgat cgatgccctt gccctggtaa aggtcgaaca gcgcgatctc
     7201 gcgtacgagc gcgggtgcgg cctcgcgcag cacctcgagc acgcgctcga ccggaacctc
     7261 gttgcccacc accagcgcaa gatcgcggcg caccgcctgc atgcgcgaga tctcggcata
     7321 ggccggcacg cgggcggcga gcgcagcatc gagcgcaacc tcgaacacca ccggcgcggt
     7381 gccgagctcg tagcgctgca cccacaccgg atgcagttca ccgagcacgc cgatcatgtg
     7441 cccgtccagg cgcacggcgg ccgcgcgccc cggatgcagg gcgggatgcg agagcggggc
     7501 gaactgcagc tcgcgcggcg cgaacagcgc ctcgagatcg cccttcacgt cgtaaaagtc
     7561 gacgctgcgc gcgcgctcgc cccactgctc gggcaccacc tggccggccg ccagcgcggc
     7621 gatgttcatc acctgatgga agccgcgcac cggctcgcca tcggccttgc gctcgaacac
     7681 gcgtgccacc tcgaaaacgc gcacgcggtc gatctggcgc ttgcgattgg cggcgagcgt
     7741 gccgaccagg cccggaatca ggctggagcg catcacgctg aggtggctgg cgatcgggtt
     7801 ggccaggcgg atcgggttct cgttggcgca gaagtcgcgc tcccaggcct cctcgacgaa
     7861 ggcgaagttg accacctcct ggtagccgcg cgcggcgagc aggtgacgca gcgcccacac
     7921 cgggcggccg ttctcggtgc tcggcagcat catcaggctg ccctgcgggg cgaccgccgg
     7981 aatgttgtcg tagccgtaca ggcgggcgat ctcctcgatc agatcttcct cgatctcgat
     8041 atcgaagcgc cacgacggcg gggtgacgag gaagtcatcg ccgacacact ccacctgcaa
     8101 gtgcacccga cgcagcagcg cgagcatctc cgcgtcgacg agcgcgatgc cgagcacgcg
     8161 gcgcgcacgc gccggacgca ggcgcaccgg cgcacgggcg ggcagcgcgt cggtggcgac
     8221 cgcctcgacc
//
LOCUS       DAOQNN010000116         8221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4428_length_8221_cov_9.141807, whole genome shotgun sequence.
ACCESSION   DAOQNN010000116 DAOQNN010000000
VERSION     DAOQNN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4428_length_8221_cov_9.141807"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKV97_07970"
     CDS             complement(<1..277)
                     /locus_tag="PKV97_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNB05851.1"
                     /translation="MSAEDCPAVLFVDEWLLVVDKPAGLLSVPAGGEGRQDCLAARIQ
                     ARWADARIVHRLDAATSGLVLFARGAEMLRRMSLAFEQRIVGKQYVAV"
     gene            complement(400..1656)
                     /locus_tag="PKV97_07975"
     CDS             complement(400..1656)
                     /locus_tag="PKV97_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HNB05852.1"
                     /translation="MSKKLAGLIVLGLASATPAFAAGQAEAKGLVEDSSLNVLLRNAF
                     INRDYKGGRHDSGEWGQGAIANFESGFTQGTVGFGVDAFAAYAVRLDGSRDRAGNWGI
                     DFFKQDSTGMPEKDVAKGGASLKLRFSNTVLAYGDLRPNLPVLSHDNSRLLPESYTGT
                     MLTSTEIEGLEFNAGTFHAQSRKSDEGRDSGGLKGIDVIGASYKFNDAFSGAFYASDV
                     EDVLKRQYLNLNYVLPIASNSLTFDFNGYRTKQDADFQPGKSRIWSLASTYATGPHAF
                     TLAYQRNNGDIDYNYGFYQSEGWIGDGGGSIWLANSYWSDFNAADEQSWQVAYTVDFA
                     AYGLPGLSYRIAYVRGHDIDVGTTTDGKEHELFNQLSYKVQSGAAKDLNVRLRASTLR
                     VSDNARAYNSSGNEVRVFLDYPFSVF"
     gene            2184..4358
                     /gene="katG"
                     /locus_tag="PKV97_07980"
     CDS             2184..4358
                     /gene="katG"
                     /locus_tag="PKV97_07980"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNB05853.1"
                     /translation="MSENSTNTAGKCPVMHGGNTAAKDAVMAWWPKALNLDILHQHDR
                     KTNPLGEDFDYREELKKLDVDALKNDLKALMTDSQEWWPADWGHYGGLMIRMAWHAAG
                     TYRIADGRGGGGTGNQRFAPLNSWPDNGNLDKARRLLWPIKKKYGNKISWADLIILAG
                     NMAYESMGFKTFGFAFGREDIWHPEKDIYWGSEKEWLAPSGSEGSRYSGQRDLENPLA
                     AVMMGLIYVNPEGVDGKPDPIKTAHDMRVTFARMAMNDEETVALTAGGHTVGKAHGNG
                     SAAVLGPAPEGADLEEQGFGWMNHTTRGIGRDTVTSGIEGAWTSHPTKWDNGYFDMLF
                     KHDWWLQKSPAGAWQWEPVNIAEEDMPVDVEDPSIRRKPMMTDADMALRFDPAYRKIA
                     ERFRNDQAYFEDVFARAWFKLTHRDMGPKARYLGPDVPAEDLIWQDPVPAGNKTYDVA
                     AVKARIAAAGLSVGDMVATAWDSARTFRGSDYRGGANGARIRLAPQKDWEGNEPARLA
                     RVLAAYEKIAAETGASIADVIVLGGNVGLEQAIKAAGFNVEVPFAPGRGDASQAQTDV
                     ESFEVLEPVHDGFRNWQKKDYVVQPEEMLLDRAQLLGLTAPEMTVLIGGLRVLGTNHG
                     GSGHGVFTDRVGALTNDFFVNLTDMSYSWKPTGRNSYDIVERKSGAKKWTATRVDLVF
                     GSNSILRAYAEVYAQDDNQEKFVRDFVAAWTKVMNADRFDLR"
     gene            complement(4733..5254)
                     /locus_tag="PKV97_07985"
     CDS             complement(4733..5254)
                     /locus_tag="PKV97_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HNB05854.1"
                     /translation="MNTRRSPDYLAGYPPALVHQVRTLIEQGGLADALRRRYPQGHAV
                     RSDSALHDYVQALKAAHLRSAPPLAKVRFDNTLHVIRNALGTHTAISRVQGSRLQAKR
                     EIRIATLFRTAPEAFLRMIVVHELAHLRVREHDKAFYQLCLHMEPDYHQYEFDLRTYL
                     SYRDAGGEALWGA"
     gene            complement(5251..6129)
                     /locus_tag="PKV97_07990"
     CDS             complement(5251..6129)
                     /locus_tag="PKV97_07990"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNB05855.1"
                     /translation="MSTDSAALHYDTIARAIAFVRARPRHTPPSGAVAEALGLDLGQW
                     QQVLAEWAGITPERFLHCLGRAYARERLSPTPERLPERDAGDPAPSDLARAVPLTWEP
                     MPPASGRTVALGHGLAATPFGDALLAWTTRGVCHLAFRCRDEAALLVELRSLWSAAAL
                     VRDDAHAHRLAQRIFPSRAPSTPVAAATGASGPAEAANPAPGGVHLILRGTPFQVRVW
                     SALMRIPPGRLMSYRQLATLLGMPAAPRSVGSAIAANTLGYLIPCHRLIRADGDAGEF
                     RWGRERKLAMLAWEAT"
     gene            6458..6772
                     /locus_tag="PKV97_07995"
     CDS             6458..6772
                     /locus_tag="PKV97_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HNB05856.1"
                     /translation="MPILLQVDFPYAGPWGEDMAQAMRGLAESIAQEPGLLWKIWTEN
                     RDSGEAGGIYLFADRADAERYLEMHRARLLGFGVPEVHAKLFEVNAPLSAIDRAPLAG
                     LQ"
     gene            complement(6836..>8221)
                     /locus_tag="PKV97_08000"
     CDS             complement(6836..>8221)
                     /locus_tag="PKV97_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNB05857.1"
                     /translation="PGIGGGSARSYFLVLEEGGEAARAICVLPRGTESGTEVALPERR
                     FALRLGQPVRFHLVSSTGDRAWQAGELVELDADDFIHLPPLAARLPATAAGRRENVAV
                     RLVACLTEVGTLEMQCVATDDDTQRWQLAFQVRAEADTPATEAAAGAHPRLAQAIALI
                     DEVFGGQARELDAKAVRQLRARLERVLGRRDDWDMALLRALFDALLERARRRRRSAEH
                     ERVWLNLAGYCLRPGLGAALDDWRVDQLWALFGQGLQYVREGNNWSEWWTLWRRCAGG
                     LDEARQLQVLEVLAEHLEDTDAARRRGDPVQGSYDDMVRLAAALERVPAMHRIEVGKW
                     LLERLARPAEKAHTWWALGRVGARESLYGSAHTVVPPETAAEWLQAVLALDWKVVEPA
                     AFAAAQLARLTGDRARDLAPELRDEVARRLAALRAPQSWITMVREVVSLDDADRRRSF
                     GEALPPGLRLL"
BASE COUNT         1297 a         2751 c         2952 g         1221 t
ORIGIN      
        1 ccacggccac gtactgcttg ccgacgatgc gctgctcgaa ggcgaggctc atgcggcgca
       61 gcatctccgc gccgcgcgcg aacagcacca gaccggaggt ggcggcgtcg agccggtgca
      121 cgatgcgggc atcggcccag cgcgcctgga tgcgggcggc aagacaatcc tgccggccct
      181 ccccgcctgc ggggacggag aggaggccgg caggcttgtc gacgacgagg agccactcgt
      241 cgacgaacag tacggctggg cagtcttcgg cagacatggt ggaacgggcc tccgggagcg
      301 tccgcgcggg attcatcaag aaaaaacggg agccagggct cccgtttgca cggccgtgca
      361 gcgtgtcggc agtgaccgat gcgctgcgca tggcgtgggt cagaacacgc tgaaggggta
      421 gtcgaggaag acgcgcactt cgttgccgct ggagttgtag gcacgggcgt tatccgagac
      481 gcgcagcgtc gatgcgcgca ggcggacgtt caggtccttg gcggcgccgc tctgcacctt
      541 gtacgacagc tggttgaaga gctcgtgctc cttgccatcc gtggtcgtac cgacgtcgat
      601 gtcgtggcca cggacgtagg cgatgcggta gctcaggccg ggaaggccgt acgcggcgaa
      661 gtcgacggta taggccacct gccaggactg ctcgtccgca gcgttgaagt cggaccagta
      721 ggagttggcg agccagatcg agccgccgcc gtcaccgatc catccctcgc tctggtagaa
      781 gccgtagttg tagtcgatgt cgccattgtt gcgctggtag gccagcgtga aggcatgcgg
      841 gccggtcgcg taggtcgagg ccaggctcca gatgcggctc ttgccgggct ggaagtcggc
      901 gtcctgcttg gtgcggtagc cgttgaagtc gaaggtcagc gagttgctgg cgatcggcag
      961 cacatagttc aggttgaggt actggcgctt gagcacgtcc tcgacgtccg aggcgtagaa
     1021 cgcgccgctg aaggcgtcgt tgaacttgta gctcgcaccg atcacgtcga tgcccttcag
     1081 gccaccgctg tcacgaccct cgtcgctctt gcgcgactgg gcgtggaagg tgccggcatt
     1141 gaattccagg ccttcgatct cggtcgaggt cagcatggtg ccggtatagc tctcgggcag
     1201 caggcgggag ttgtcgtggc tcagcaccgg caggttgggg cgcaggtcgc cgtaagcgag
     1261 cacggtgttc gagaagcgca gcttgaggga ggcgccaccc tttgccacat ccttctccgg
     1321 catgccggtg ctgtcctgct tgaagaagtc gatcccccag ttgcctgcgc ggtcgcggga
     1381 gccatcgagg cgaaccgcat aggcggcaaa tgcgtccaca ccgaatccga ccgtgccctg
     1441 tgtgaagccc gactcgaagt tcgcgatcgc accctggccc cattcgccac tgtcgtggcg
     1501 gccgcccttg taatcacgat tgatgaacgc gttacgcagc agcacgttca gcgagctgtc
     1561 ttccacgaga cccttcgcct cggcctgccc ggcggcgaat gcgggcgtcg cgctcgcgag
     1621 cccaagaacg atcaaaccag ccaacttctt cgacatgttg ttttcctcga tggtgtccgt
     1681 tgtcttttcc agcctgccgg cgggtcgtgc ccgccggaga gggggcgcga ttctcgcatt
     1741 tccgacgcga agcggtgcgg cgggttgcag gaaaatgaca gtccctggcc gaacaaggtt
     1801 ggcgaccccg gagtgtcggg ttttcttaca ctcggctata gagaaaactg ctcgaactgg
     1861 atagccaggc tgggtttcgt gcgcgcgacc ggcagggcgt tcgcacctct gctggagggt
     1921 gtgggggcgc atgagcggaa cgcgttccgc aggccgaccg gataaatttt attgtgcacc
     1981 gcagcgtcgg aagatagtct ccgtcaattc tctccattgc ttccaaagcg cccgatcttt
     2041 gctagtctga gcctgtcagc agcggttttc ggccgtttcc ggccttgttc cgcgatcgtg
     2101 gagcgagctt cggaagcggt tttctgcgag gtgcgaccgc ccctcgcaat cagtctgcgt
     2161 cacagtcaca gagggagaat cagatgagcg agaacagcac gaacaccgcc ggcaagtgcc
     2221 cggtgatgca tggcggcaat accgccgcca aggatgcggt gatggcgtgg tggcccaagg
     2281 cgctgaacct ggacatcctc catcagcacg accgcaagac caacccgctc ggcgaagact
     2341 tcgactaccg cgaagagctg aagaagctcg acgtcgacgc gctcaagaac gacctcaagg
     2401 cgctgatgac cgacagccag gaatggtggc cggccgactg gggccactac ggcggcctga
     2461 tgatccgcat ggcctggcac gccgccggca cctaccgcat cgccgacggt cgtggcggcg
     2521 gcggcaccgg caaccagcgc ttcgcgccgc tgaactcctg gcccgacaac ggcaacctcg
     2581 acaaggcgcg ccgcctgctg tggccgatca agaagaagta tggcaacaag atcagctggg
     2641 ccgacctcat catcctcgcc ggcaacatgg cctacgagtc gatgggcttc aagactttcg
     2701 gcttcgcctt cggtcgcgag gacatctggc atcccgagaa ggacatctac tggggctccg
     2761 agaaggaatg gctcgccccc agcggcagcg agggctcgcg ctactccggc caacgcgacc
     2821 tcgagaatcc gctcgccgcg gtgatgatgg gcctgatcta tgtgaacccg gagggcgtcg
     2881 acggcaagcc cgacccgatc aagaccgccc acgacatgcg cgtgaccttc gcccgcatgg
     2941 cgatgaacga cgaggaaacc gtggcgctca ccgccggcgg ccacaccgtc ggcaaggcgc
     3001 acggcaacgg tagcgccgcc gtcctcggcc ccgcgcccga aggtgcggat ctcgaggagc
     3061 agggcttcgg ctggatgaac cacaccaccc gcggcatcgg tcgcgacacc gtgaccagcg
     3121 gcatcgaggg cgcgtggacc tcgcatccga ccaagtggga caacggctac ttcgacatgc
     3181 tgttcaagca cgactggtgg ctgcagaagt cgcccgccgg cgcctggcag tgggagccgg
     3241 tgaacatcgc cgaggaagac atgccggtgg acgtggaaga cccgtcgatc cgccgcaagc
     3301 cgatgatgac cgacgccgac atggcgctgc gattcgaccc ggcgtaccgc aagatcgcag
     3361 agcgcttccg caacgaccag gcctacttcg aggacgtctt cgcgcgcgcc tggttcaagc
     3421 tcacccaccg cgacatgggc ccgaaggcgc gctacctcgg tcccgacgtg cctgccgaag
     3481 acctgatctg gcaggacccg gtgcccgccg gcaacaagac ctacgacgtc gccgcggtga
     3541 aggcgaggat cgccgccgcc ggcctgtcgg tcggcgacat ggtcgccacc gcctgggaca
     3601 gcgcacgcac cttccgcggc tcggattacc gcggcggcgc caatggcgcc cgcatccgcc
     3661 tcgccccgca aaaggactgg gaaggcaacg aaccggcgcg gctggccagg gtgctggcgg
     3721 cctacgagaa gatcgccgcc gagaccggcg ccagcatcgc ggacgtgatc gtgctcggtg
     3781 gcaacgtcgg cctggagcag gcgatcaagg ccgccggctt caatgtcgag gtgcccttcg
     3841 ccccgggtcg aggcgacgcc agccaggcgc agacggacgt cgagtccttc gaggtgctgg
     3901 agcctgtgca tgacggcttc cgcaactggc agaagaagga ctatgtggtc cagcccgagg
     3961 aaatgctgct cgaccgcgcc caactgctcg gcctgaccgc gcccgagatg accgtgctga
     4021 tcggcggcct gcgcgtgctc ggcaccaacc acggcggcag cgggcacggc gtgttcaccg
     4081 accgcgtcgg cgcgctgacc aacgacttct tcgtcaatct gaccgacatg tcctacagct
     4141 ggaagcccac cgggcgcaac agctacgaca tcgtcgagcg caagagcggc gcgaagaagt
     4201 ggactgcgac ccgcgtcgat ctggtgttcg gctccaattc catcctgcgc gcctacgccg
     4261 aggtgtatgc ccaggatgac aatcaggaga agttcgtgcg cgacttcgtc gccgcgtgga
     4321 ccaaggtcat gaacgcggat cgtttcgacc tgcgctgacg cggttcccgc ctgcagcacc
     4381 ctcccgccca caccgtgcag ccgcgcggtg tgggcggttt cgttcgggga cggccgggat
     4441 gtgcccctcg ccacgccgtg tcgagccgtg ccggccgatc gcgactcgcg tcgctcccac
     4501 ggaaaaccgc cgtgcaggcc gatcgcgact tgcgtcgctc ctacggaaaa ccgccgcgcc
     4561 ggccgatcgc gactcgcgtc gctcccacgg agaaccgccg cgccggccga tcgcgacttg
     4621 cgtcgctcct acggatagcc gccgtgcttc cgccgtgccc ggtgggagcg acgcaagtcg
     4681 cgatcgtgcg gtgcggccgc gacgcgcgct gcgtatctgg actccggcat ccctacgccc
     4741 cccacagcgc ctcgccgccc gcgtcgcggt agctcaggta ggtgcgcagg tcgaactcgt
     4801 actggtgata atccggctcc atgtgcaggc agagctggta gaaggccttg tcgtgctcgc
     4861 gcacgcgcag gtgggcgagc tcgtgcacca cgatcatgcg caggaaggcc tccggcgccg
     4921 tgcggaacag cgtggcgatg cggatctcgc gcttggcctg gaggcgcgaa ccctgcacgc
     4981 gcgagatcgc ggtgtgggtg ccgagcgcgt tgcggatcac atgcagggtg ttgtcgaagc
     5041 gcaccttggc gagtggcggc gcgctgcgca ggtgggcggc cttgagcgcc tgcacgtagt
     5101 cgtggagcgc gctgtccgaa cgcaccgcgt gcccctgcgg gtagcgtcgg cgcagcgcgt
     5161 cggccagtcc gccctgctcg atcagcgttc tcacctgatg caccagcgcc ggtgggtagc
     5221 cggccaggta gtcggggctg cgccgcgtgt tcaagtggcc tcccaggcca gcatcgccag
     5281 cttgcgctcg cggccccagc ggaactcgcc cgcgtcgccg tccgcgcgga tcaggcgatg
     5341 gcaggggatg aggtagccga gcgtgttggc cgcgatcgcg ctgccgacgc tgcgcggtgc
     5401 agccggcatg ccgagcaggg tggcgagctg gcggtaggac atcaggcggc cgggcgggat
     5461 gcgcatgagg gcggaccaga cgcggacctg gaagggcgtg ccgcgcagga tcaggtgaac
     5521 gccgcctggg gcggggttcg ccgcttcggc gggcccggaa gccccggtgg ctgcagccac
     5581 cggggtggaa ggcgcgcgcg acgggaagat ccgctgcgcc aggcggtgtg cgtgcgcatc
     5641 gtcgcgcacc agcgcggcgg cggaccagag gctgcgtagc tcgacgagca gggccgcctc
     5701 gtcacgacag cgaaaggcga gatggcacac gccgcgggtg gtccacgcca gcagcgcgtc
     5761 gccgaagggc gtggctgcaa ggccgtggcc gagcgcgacg gtgcgccctg aggcgggggg
     5821 catcggctcc caggtgagcg gcacggcgcg ggccaggtcc gacggtgcgg gatcgccggc
     5881 gtcgcgctcc gggagccgct ccggcgtcgg cgacaggcgc tcgcgtgcat acgcccgccc
     5941 caggcagtgc aggaagcgct cgggcgtgat ccccgcccat tccgcgagca cctgctgcca
     6001 ctgcccgaga tcgaggccaa gcgcctccgc gaccgccccg gacggtggcg tgtggcgggg
     6061 gcgggcgcgc acgaaggcga tcgcgcgggc gatggtgtcg tagtgcaggg cagcggagtc
     6121 ggtggacatg gcgggtgcgg gtgtgatggg tgacaaggcg gggtggcctt gcggtcgaca
     6181 atggtagccg gcctggccgg cgaggcttgt gcaggacgcg ctgctatcgc gactggcgtc
     6241 gctcctgcgg gcgctccgga aacctgcccg gttttccata ggagacgcaa gtcgcgatgc
     6301 gtcggttcgt ttggcgccgc gaccggatac ctgcgccgcg acggtagaat ggccgatgaa
     6361 tccggagccg tcccgaaccg tgcgaacgcc gcgctccacc ctcccggcgc gacccggctt
     6421 gccttccgtt ccgaccccga cgcacaggag atcgcgcgtg cccatccttc ttcaagtcga
     6481 ttttccctat gccggcccct ggggcgagga catggcgcag gccatgcggg gcttggcgga
     6541 gtccatcgcg caggagcccg gcctgctgtg gaagatctgg accgagaacc gcgacagcgg
     6601 cgaggcgggc gggatctacc tgttcgccga ccgcgccgac gccgagcgct acctggagat
     6661 gcaccgcgcg cgcctgctcg gcttcggcgt gcccgaggtg catgcgaagc tgttcgaggt
     6721 caacgcgccg ctgtcggcga tcgaccgcgc gccgctcgcc ggcctgcagt gaccggatgg
     6781 gcgccgtcgg catcgagccg gcgacggcgc gccctcctcc ctccaccgcg cagcgctaca
     6841 gcagccgcaa gcccggcggc agcgcttcgc cgaagctgcg gcggcggtcg gcgtcgtcga
     6901 ggctgacgac ctcgcgcacc atggtgatcc agctctgcgg ggcgcggagg gcggcgaggc
     6961 ggcgcgcgac ctcgtcgcgc agttcgggcg cgaggtcgcg cgcgcggtcg ccggtgaggc
     7021 gggcgagctg ggcggcggcg aaggcggcgg gctcgacgac cttccagtcg agcgcgagca
     7081 cggcctgcag ccactcggcg gcggtctccg gcggcaccac ggtgtgtgcg ctgccgtaga
     7141 gcgactcgcg cgcgccgacg cggccgagcg cccaccaggt gtgggccttt tcggcggggc
     7201 gggcgaggcg ctcgagcagc cacttgccga cctcgatgcg gtgcatcgcc ggcacgcgtt
     7261 cgagggcagc ggccaggcgc accatgtcgt cgtagctgcc ctgcaccggg tcgccgcggc
     7321 ggcgggcggc gtcggtgtct tcgaggtgct cggccagcac ctccaggacc tgcagctggc
     7381 gtgcctcgtc gaggccgccg gcgcagcgcc gccacagcgt ccaccactcg ctccagttgt
     7441 tgccctcgcg cacgtactgc aggccctggc cgaacagcgc ccacagctgg tcgacgcgcc
     7501 agtcgtcgag cgccgcgccg aggccgggac gcaggcagta gccggcgagg ttgagccaca
     7561 cgcgctcgtg ctcggcggag cgccggcgcc ggcgcgcgcg ctcgagcagg gcgtcgaaga
     7621 gcgcgcgcag cagcgccatg tcccagtcgt cgcggcggcc gagcacacgc tcgaggcggg
     7681 cgcgcagctg gcgtaccgcc ttggcgtcga gttcgcgcgc ctgcccgccg aagacctcgt
     7741 cgatgagggc gatggcctgg gcgaggcgag gatgggcgcc ggccgcggcc tcggtggcgg
     7801 gcgtgtcggc ctcggcgcgc acctggaagg cgagctgcca gcgttgcgtg tcgtcgtcgg
     7861 tcgccacgca ctgcatctcc agcgtgccga cctcggtcag gcaggcgacc aggcgcacgg
     7921 cgacgttctc gcggcgtccg gcggcggtgg cgggcaggcg ggcggcgagc gggggcaggt
     7981 ggatgaaatc gtcggcgtcg agctcgacca gctcgcccgc ctgccacgcg cggtcgccgg
     8041 tcgaggacac gaggtggaag cgcaccggct ggccgaggcg cagcgcgaag cgacgctcgg
     8101 ggagcgcgac ctcggtgccg gattcggtgc cgcgcggcag cacgcagatc gcgcgcgcgg
     8161 cctcgccgcc ctcctccagc accaggaagt agctgcgcgc cgagccgccg ccaatgccag
     8221 g
//
LOCUS       DAOQNN010000117         8039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4594_length_8039_cov_5.815631, whole genome shotgun sequence.
ACCESSION   DAOQNN010000117 DAOQNN010000000
VERSION     DAOQNN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4594_length_8039_cov_5.815631"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..862)
                     /gene="istB"
                     /locus_tag="PKV97_08005"
     CDS             complement(116..862)
                     /gene="istB"
                     /locus_tag="PKV97_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNB05858.1"
                     /translation="MSITEPVLMRQLARLQLPYFVQQCAPLCAQAARESWTHARFLEQ
                     LVAGEVARRDEALVDRRVKAARLPGIKTLDGFDWTWPKKINRAQVQHLFRLDFIPRNG
                     NVIFLGGVGIGKTHLAVALAHTACLRGATTLFTSAVDIVNSLAAAQAIGGIKRELARL
                     TKPSLLIIDELGYLPIDKFGADALFQVISQRYERGSTVITTNRAFKLWPEIFNNDSTL
                     TSALLDRLLHHADSIVIEGPSYRMREHNNA"
     gene            complement(849..1556)
                     /locus_tag="PKV97_08010"
     CDS             complement(849..1556)
                     /locus_tag="PKV97_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05859.1"
                     /translation="MANVRMHGTTHCRPADLWAEERLHLQAVNPRPFDVGRVLSVRAN
                     RQFRITFDSNRYSVPARFAGYPLTLKAYPDRLCVYHEQELVARHVRSYDRHQDIEDPD
                     HPKVLLAQRRHAHDAHVLKRFLGLSPLATKYHAGLLERRGNAISHLRKIVALADIHGD
                     EVVVRALTDALAFEAFSSEYIDHLIQVRGRQLPEASPLVLVRRQDVLDLELPPPDLSA
                     YSAMATDFNPGDDRVDH"
     gene            complement(1689..2411)
                     /gene="istA"
                     /locus_tag="PKV97_08015"
     CDS             complement(1689..2411)
                     /gene="istA"
                     /locus_tag="PKV97_08015"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNB05860.1"
                     /translation="MIDYATWCAIRDGAARHLTPPQLADTLGLDVKTVRHWIDRPYQQ
                     RARAQRPSKLDPFKGRIVGWLDAHPLSAQQVFQRLQEAGYQGGVSIVKDYVRAIRPPR
                     REAFLTLAFAPGEAVQVDWGAWGSIAVGNTCRKLSFFVMVLTYSRKMYVEFMLAQTME
                     NFLAAHINGFNALGVAQKVIVDNLRSAVLLHVRGEAPRFHPRYLDFARHYGFEPVACN
                     VGKGNDQEPKVPQDAGFAGGLA"
     gene            complement(2408..2773)
                     /locus_tag="PKV97_08020"
     CDS             complement(2408..2773)
                     /locus_tag="PKV97_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05861.1"
                     /translation="MNVSKRVLDLKRLRQTPAHFSWLDHRLVREHLIEQADVCAWALY
                     LVLVCVADGHGLSYYGDASLCRRLRLSPERLVQARRDLVALDLIAYDAALYQVLSLPE
                     LVPVAVRCERLRSLLEGRP"
     gene            complement(3905..7666)
                     /locus_tag="PKV97_08025"
     CDS             complement(3905..7666)
                     /locus_tag="PKV97_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB05862.1"
                     /translation="MRDLLIGVTSFFRETKAFEMLISTIAPAIVERARGEYPVRIWVP
                     GCASGEEAYSLAMVFMEAIRRSGKRVSLQIFATDIDSEALEIARAGIYPVSATADLPS
                     EYVQQFFVREGEHVSVIKPVRESVVFAVQNLITDPPFSRLDLVSCRNLLIYLDNEVQA
                     KVIRLFHFALRENGYLFLGSSETIGLRTDMFRTISKKWRIYHRLQTPSDARGTDYYPL
                     VSDAAVITPRTAGDRVRGPVGRRLGERVEEKLLGEYAPAAVLVDRNGEALYFHGATSN
                     YLGMPTGSATHDVVSLAREPLRMTLRAALQRAMREKMKIEARQVAVGDRGNERKVTIK
                     VWPLDENEGTFLVCFEDDDEPGPASQPRASEEALVTQLEYELKATREDLQTTIEEVET
                     TNEELKAANEEVMSMNEELQSTNEELETSKEELQSLNEELSTVNNQLLDKVQELESAN
                     NDLANLLTRTDIATIFLDLELRIRRYTPTATRMFALIDADHGRPIVDVARRFSDGDLS
                     EHCRQVLAELKTFQEEVLGSDGSWFLLRILPYRTSDNRIGGVVITFSDISEIKAAYAE
                     ARTRESQLRIVADAMPALIAHVDLEHRFRFVNAAYENWFGVPPQRILGEPLCKVFGTL
                     AYGNLCPHLQQAMGGKTVTASAQVVHCRLGQREVTEIWIPEFGPGGRVAGFFSLIQDV
                     TEQRLAERKLVAADMVFRNTTEAAAILDQEGRFVTVNPAFEQVTGYPFTQVEGDGWDL
                     LLAERHAPEPFESIDKSMNTGKGWRGEATLRRKGGGVFAAWMSINPVQPRGSEVDGFV
                     VIVADISTVKEAERRLEYLAHHDPLTGLSNRVMFQERVAQLISHAGRHEKVVGLLYLD
                     LDGFKHVNDSFGHEVGDKVLVLAARRLEECMRAEDTLARLGGDEFGVVLEDVTEPWGV
                     NLVAEKIIEGLRAPFEVEGQTLVLGVSIGISLYPSDGKHAPILIRNADTAMYRAKERG
                     GNISHFYTPALTEAVQQRLSTESALRQAIVEGELELYFQPIMEIASERIVAAEALLRW
                     RTRDRGLILPESFLPVAEMSGLIIQVGVHVIELACKALAQWREQSLPVPRLSLNVSAR
                     QCMDDEFPEVVAQALASFDIPAASVDFEITESSFLDKGTSRKVLAALRGLGLSLTIDD
                     FGTAYATLASLRNIPVSAIKIDRAFIDDIGNSSGDDAIASAVIALGKSQNLRVIAEGV
                     ETMAQLEALRALGCDDCQGYLIAPPMSAKAFASWWQKNGERMVEANPAD"
BASE COUNT         1407 a         2522 c         2672 g         1438 t
ORIGIN      
        1 cgacgtgaat gtggtgtgtt ggttgagggt gtgacgtcac cgggaaggca caaacggagg
       61 caccgccttt cacgcgaggt gggcggtggt ggcgagaatc ggagaggcga cggaattacg
      121 cgttgttgtg ctcgcgcatg cgatagcttg gcccctcgat cacgatcgag tcggcgtgat
      181 gcagcaggcg atcgagcaag gccgaggtga gcgtgctgtc gttgttgaag atttcgggcc
      241 acagcttgaa cgcgcgattg gtcgtgatga ccgtcgaacc acgctcgtag cgctggctga
      301 tcacctggaa gagcgcgtcg gcgccgaact tgtcgatggg cagatagccg agttcgtcga
      361 tgatgagcag ggacggcttg gtcagccgag cgagctcgcg cttgatgccg ccgatggcct
      421 gcgcggcggc gagcgagttg acgatgtcga cggcgctggt gaagagggtg gtcgcgccac
      481 gcaggcaagc ggtgtgcgca agcgcgacgg cgaggtgtgt cttgccgatg ccgactccgc
      541 ccaggaagat cacgttgccg ttgcgcggga tgaaatccag acggaacaga tgctgcacct
      601 gcgcacggtt gatcttcttc ggccaagtcc agtcgaagcc gtcgagggtc ttgattcccg
      661 gcaatctggc ggccttgacg cgacggtcga cgagcgcttc gtcgcgacgg gcgacctcgc
      721 cggcgacgag ctgttcgagg aaccgtgcgt gcgtccagga ttcgcgagcc gcttgtgcgc
      781 acaggggcgc gcactgctga acgaagtacg gcaactgcaa gcgcgcgagt tggcgcatga
      841 ggacgggctc agtgatcgac acgatcgtct cccgggttga aatcggtggc catggccgaa
      901 tacgccgaca ggtcgggcgg cggcagctcg aggtcgagga cgtcctggcg ccgcaccagg
      961 accagtggac tcgcctcggg cagttggcgg cctcgcacct ggatcaggtg gtcgatgtac
     1021 tcgctgctga acgcttcgaa cgccagggcg tcggtgagcg cccgcaccac gacctcgtcg
     1081 ccgtggatgt cggccagcgc aacgatcttg cgcaagtgcg agatcgcgtt gccgcgacgt
     1141 tcgagcaggc cggcgtggta cttcgtcgcg agcggagaca ggccgaggaa gcgcttgagc
     1201 acgtgggcat cgtgtgcgtg gcggcgttgc gcgagcagca ccttgggatg gtcggggtcc
     1261 tctatgtcct ggtggcgatc gtaagagcgg acatggcggg cgaccagctc ctgttcgtga
     1321 tagacacaga gccgatccgg atacgccttg agcgtgaggg gatagccggc aaagcgggcg
     1381 ggaacggaat agcggttgct atcgaaggtg atgcggaact ggcggttggc acgcaccgac
     1441 agcaccctgc cgacatcgaa cgggcgggga ttgaccgctt gcaggtgcag acgctcttcg
     1501 gcccacaggt cggccggtcg gcagtgggtg gtgccgtgca tccgcacgtt ggccaccgat
     1561 tccagccaga cctgcgccgc gggattgagc gcggcgaacg gcggcaggtc gaggcctctg
     1621 aggaagttct ccttgatgta gccgacgcct cgctcgaccc ggcccttctg tgcgctgcgc
     1681 gcatctcgct acgcgagacc gccggcaaag ccggcgtcct gcggaacctt cggttcctgg
     1741 tcattgccct tgccgacgtt gcaggcgacc ggctcgaagc cgtaatggcg ggcgaagtcg
     1801 agatagcgcg gatggaagcg cggcgcctcg ccgcggacgt ggaggaggac cgcggagcgc
     1861 agattgtcga cgatcacctt ctgcgcgacg ccgagcgcgt tgaagccgtt gatgtgcgcg
     1921 gccaggaagt tctccatggt ttgcgcgagc atgaattcga cgtacatctt gcgcgagtag
     1981 gtcagcacca tgacgaagaa ggacagcttg cggcaggtgt tgccgacggc gatgctgccc
     2041 cacgcgcccc agtcgacctg aaccgcctcg ccgggcgcga aggcgagcgt caggaaggcc
     2101 tcgcgcctgg gcgggcggat ggcgcgcacg tagtctttga cgatactgac gccaccctgg
     2161 taacccgcct cctgcaggcg ctggaagacc tgctgtgccg acagtggatg cgcgtcgagc
     2221 cagccgacga tgcgcccctt gaacgggtcg agcttgctcg gtcgctgtgc gcgcgcacgc
     2281 tgctgatagg ggcgatcgat ccagtggcgg acggtcttga catcgagccc cagagtgtcg
     2341 gccagttgcg gcggcgtgag atgtctcgcc gcgccgtcgc ggatcgcgca ccaagtcgca
     2401 tagtcgatca tggccgtccc tccaacagcg agcgcaggcg ctcgcagcgt accgccaccg
     2461 ggacgagttc aggcaggctc agcacttggt agagcgcagc gtcgtaggca atcaggtcga
     2521 gcgccacgag atcccgacgc gcttgtacca gtcgctcggg agacaagcgc aggcgccggc
     2581 acagactcgc gtcgccgtag tagctcagcc cgtggccatc ggcgacgcag accaggacca
     2641 ggtacaaggc ccaagcgcag acgtcagcct gttcgatgag gtgctcgcgc accaggcggt
     2701 ggtcgagcca actgaaatgc gcgggggtct ggcgcagccg tttgaggtcc agaacccgct
     2761 tgctcacgtt catgatcgcc tctccggatg tcctgcccca gttgtagcag ccgggccaat
     2821 gccgaggcga tgtcctcttg taacgcactg ccccaaagat gggcgattaa gccaatcaga
     2881 acaattggtt gagaactcaa gacatcttgt aacggcggcg ccggagcgtt acaagatatc
     2941 ctcggcggcg gcgcctccgg cagcgtggcc gcaggcatgt cgaccgcagg cgatcggttg
     3001 tcagtcacag gcgttgccgg cggttcggct tggcgccggc tgtagcccgg atgcgccttg
     3061 cgccatacct ggacccgcgc gaccgcttcc ggcccgtggt agtagccctc gttctccggt
     3121 ttggccagcc acttcgcctg actcgcacgc ttgctcgccg ccttgcacgg cacggccgtg
     3181 cagtagcgct ggtgatgtgc gttgcgcaca tccggaacaa acggcgatcc gcaattcaca
     3241 cacctccgct gatcgcttcg cctcacgtcg caccctcgcc cggaccatga cgaggcaatt
     3301 acacgaccaa aagaaaagcg aaaaaaagac cgagagcgaa gaagacgcca gcttgaattg
     3361 acccccgctc gaagaagacg ggcaccgcgg aaacaccggt ttcacatcgg caggtttacg
     3421 ccggtttcag gtcggcggtg acagacgccg gccgcgggcc ggcgctgcga acgaaccgca
     3481 ggatgcaata cacgaccgac gatttcctcc agcagatcag gtcttggggc atcacccgag
     3541 tttcgcgttc gttgccgaac cgcagatcag cggggtggcc gggcgcttca atcgaacctt
     3601 caaggaacag gtgatctacg gacggacgtt ccaagatatc caagcatccg cgacgctgtg
     3661 accgaattca ggaagcgcga caatcgtcat tggcggttgg agaaactggg ctccacgtca
     3721 ctactcgaag cccgccatgc gcacgccatt cgagtggccg cctgaattgc caaaccgtgt
     3781 ccaggcgatc tgcgccggta cacgcacttt tcgagcgcga cgggaccgcg cggagtactc
     3841 cttgcacgaa cacgcgtgac tgcgaacccc aagaatgtgc agggccagat atctcatttc
     3901 gcgatcaatc cgccgggttc gcttccacca tgcgctcccc gtttttttgc caccaactcg
     3961 cgaaggcctt ggcactcatc ggcggggcga tcaggtagcc ttggcagtcg tcgcatccca
     4021 gtgcgcgcag cgcctcaagc tgcgccatcg tctcgacgcc ctctgcgatc acgcgcagat
     4081 tctggctctt gccgagcgcg atgactgcgc tggcaatcgc gtcgtcgccg gaactgttgc
     4141 cgatgtcgtc gataaaggcg cgatcgatct tgatcgcgct caccgggatg ttgcgcaggg
     4201 aggcgagcgt ggcataagcg gtgccaaagt catcgatggt caagctgagt ccgagacccc
     4261 tcagcgcggc caataccttc ctcgacgtgc ccttgtcgag gaaggaactt tcggtgatct
     4321 cgaagtcgac actggctgcg gggatgtcga agctcgcgag cgcttgcgcg accacctccg
     4381 gaaactcgtc atccatgcac tggcgtgcgg aaacattgag cgacaggcgc ggtaccggca
     4441 gggactgttc gcgccactgg gcgagcgcct tgcaggccag ctcgatgacg tgcacgccaa
     4501 cctggatgat cagtcccgac atctcggcca ccgggaggaa gctctcgggc aggataagcc
     4561 cgcggtcacg ggtgcgccag cgcagcagcg cttcggcggc gacgatgcgc tccgacgcga
     4621 tctccatgat cggctggaag taaagttcga gttcgccctc gacgatcgcc tggcgcaggg
     4681 cagactcggt cgacaggcgc tgctgtaccg cctcggtgag cgctggtgtg tagaagtgcg
     4741 agatgttgcc gccgcgctcc ttggcccggt acatcgcggt gtcggcattc cggatcagga
     4801 tcggcgcatg cttgccgtcg ctcgggtaca ggctgatgcc gatgctcacg ccgagcacca
     4861 gcgtctgtcc ctcgacctcg aacggggcgc gcaggccttc gatgatcttc tccgcaacca
     4921 ggttgacgcc ccaaggctcg gtcacatcct cgagcaccac cccgaactcg tcgccgccga
     4981 gtcgtgccag cgtgtcctcg gcgcgcatgc actcctcgag tcggcgcgcg gcgagcacca
     5041 gcaccttgtc gccgacctcg tggccgaagc tgtcgttcac gtgcttgaag ccgtcgaggt
     5101 caaggtagag caggccgacg accttttcgt ggcgcccggc atggctgatt agttgggcga
     5161 cccgctcctg gaacatgacc cggttcgaga ggccggtgag cgggtcgtgg tgggcgaggt
     5221 actcgagacg ccgctcggcc tccttgaccg tgctaatgtc ggccacgatg accacgaacc
     5281 cgtccacttc gcttccccgt ggctgcaccg ggttgatgct catccaggcg gcgaagaccc
     5341 cgccaccctt gcgccgcagc gtcgcttcgc cacgccaacc cttgccggtg ttcatgctct
     5401 tgtcgatcga ctcgaaaggt tcgggtgcgt ggcgttcggc aagcagcagg tcccagccgt
     5461 caccctcgac ctgcgtgaac gggtagccgg tgacctgctc gaaggccgga ttgaccgtga
     5521 cgaagcgccc ctcctgatcc aggatcgcgg cggcctcggt ggtattgcgg aacaccatgt
     5581 cggcggccac cagcttgcgt tcggccagcc tctgctcggt cacgtcctgg atcagcgaga
     5641 agaagcccgc gacccggccg ccgggaccga actccgggat ccagatctcc gtgacctcgc
     5701 gctggcctag gcggcagtgc accacctgcg cgctcgcggt caccgtcttg ccacccatcg
     5761 cctgctgaag gtgtgggcac agattgccgt aggccagcgt accgaagacc ttgcacagcg
     5821 gttctccgag gattctctgt ggcggcactc cgaaccagtt ctcgtaggcg gcattgacga
     5881 agcggaagcg gtgctcgagg tcgacatggg cgatcagcgc tggcatcgcg tcggccacga
     5941 tgcgcagctg gctttcgcgc gtccgcgctt cggcgtaggc agccttgatc tcggagatgt
     6001 cggagaaggt gatgaccacg cctccgatgc ggttgtcgct ggtgcgatag ggcaggatgc
     6061 gcagcaggaa ccagctgccg tcactaccga gcacttcttc ctggaaggtc ttgagctctg
     6121 ccagcacctg ccggcagtgc tcgctcagat caccgtcgct gaagcgccgc gcaacgtcga
     6181 cgatcggccg gccgtggtcg gcgtcgatga gcgcgaacat ccgcgtcgcg gtgggcgtgt
     6241 agcggcggat gcgaagctcg aggtcgagaa aaatggtggc gatgtcggtc cgcgtgagca
     6301 ggttagcgag gtcgttattg gccgattcga gttcctggac cttgtccagc agttggttgt
     6361 tgaccgtcga caactcctcg ttgagcgact gcagttcttc cttcgaggtt tccagctcct
     6421 cattggtgga ctgtagttcc tcgttcatcg acatgacttc ttcgttggca gccttcaact
     6481 cctcgttggt cgtctccact tcctctatgg tggtttgcag atcctcgcgg gtggccttca
     6541 gttcgtactc gagctgggta accagcgcct cttcgctggc gcggggctga gatgccggcc
     6601 caggttcgtc gtcatcctcg aagcagacca ggaacgtgcc ctcgttctca tccagcggcc
     6661 acactttgat tgtgaccttg cgttcattgc cgcgatcgcc gaccgcgacc tggcgcgcct
     6721 cgattttcat cttttcccgc atcgcgcgct gcagtgccgc gcgcagcgtc atgcgcaggg
     6781 gctcgcgcgc gagcgagacg acatcgtggg tggctgagcc ggtgggcatc cccaggtagt
     6841 tggaggtcgc gccatggaag tagagcgcct ctccgttgcg gtcgacgagc actgcggctg
     6901 gcgcgtattc gcccagcagc ttttcttcga cgcgctcgcc cagacgccgg ccgaccgggc
     6961 cacgcacccg gtcgccggcg gtgcgcggcg tgatcacggc ggcgtcgctg accagcggat
     7021 aatagtcggt gccgcgcgca tccgacggcg tctgaaggcg gtggtagatg cgccacttct
     7081 tcgagatcgt ccggaacatg tcggtgcgca ggccgatggt ttccgagctg cccaggaaga
     7141 ggtagccgtt ctcgcgcagc gcgaaatgga aaagccggat gaccttggcc tgaacctcgt
     7201 tgtccaggta gatcagcaga ttgcggcagc tgacgaggtc gaggcgagag aagggcgggt
     7261 cggtgatcag gttctgcacc gcgaacacca ccgactcacg cactggcttg atcacactga
     7321 catgttcgcc ttcgcggacg aagaactgct ggacgtattc ggacgggagg tccgcggtgg
     7381 ccgacaccgg gtagatgccc gcgcgcgcga tctcgagcgc ctctgagtcg atgtcggtgg
     7441 cgaagatctg taggctgacc cgcttgccgc tgcgccggat cgcctccatg aaaaccatgg
     7501 ccagcgaata agcctcctcg ccgctggcgc atcccggcac ccagatacgc acgggatact
     7561 caccgcgagc ccgctcgacg atggccgggg cgatcgtcga gatgagcatc tcgaaggcct
     7621 tggtttcgcg gaagaagctg gtcaccccga tcagcaggtc gcgcaccagc ttgcgcgcct
     7681 cttcctcgga gccgcgcagc aactcgaggt agtcgcgcag actcacaagc gtcgacaagc
     7741 ccatgcgacg cgccacccgg cgcccgaggg tgcctctctt gtagtgacga aagtcgtgcc
     7801 ctgtgcgggc acggacgacg ccgagcaccg cacttagatc gtccagcggc ttggcgacat
     7861 ccggctcggg ggcgggttcg ccgctcacat aagggtgctg cacatagcgg gtaagcaccg
     7921 gcgccatcgc ctccaccgcc aggcagtaat ccaccagtcc ggtggtcagc gcgctctgcg
     7981 gcatgtgaac cggtacccct tgagtggacg ccaagaggcg aggagcgtta tccacggcc
//
LOCUS       DAOQNN010000118         8011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4618_length_8011_cov_9.680116, whole genome shotgun sequence.
ACCESSION   DAOQNN010000118 DAOQNN010000000
VERSION     DAOQNN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4618_length_8011_cov_9.680116"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="PKV97_08030"
     CDS             complement(<1..1174)
                     /locus_tag="PKV97_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNB05863.1"
                     /translation="MMAHPDAASAAAPADARRVVRLIHADAPLPADCSAEALGSKAWN
                     LARMAALGLPVPPAFVIGTGWCAEPGALAEAAWRPALAELERLSGLRLGDTRHPLLLS
                     VRSGAPVSMPGMMETLLDIGLTETTLSGLLRVSGHPRLTWDAYRRLIAGYGEVVAGID
                     AQHFDADLEAVRGECGECGQGDERVLDFAALRELAARHLDTYRREAGEAFPQDAHEQL
                     RQAIAAVFASWHADKARAYRAMHGLADDMGTAVTVQRMVFGNAGGLSGAGVGFTRDPS
                     SGEPRPWVDFLFNAQGEDVVSGRRSAHGHDELAAVAPAVWQAVLDVTARLERAFGDMQ
                     DFEFTVQQGVLHLLQTRAGKRTPQAAARIALDLLDEGVIDAETARVRTAALDARALA"
     gene            complement(1201..3129)
                     /locus_tag="PKV97_08035"
     CDS             complement(1201..3129)
                     /locus_tag="PKV97_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNB05864.1"
                     /translation="MEKIEAVASLGQAELLRPARVRAALAANDRLKLYLSVLQAAHDC
                     AQRGDARALDLAREFAVAGVDAPWLAQLPATAYREGKELHVAGLDRLAGLLAEDLCTM
                     ARPLEGDADFAARCAHWCAWLEALAPDTLSSAQLHALTSGKRGKDDSLHLLVMDLHKA
                     LNRLASELSSETIDGAHVWQVDAADRSRIAAFMRGLNATRALKLDHPGLDTAATRDGA
                     RLLLQNDIGTNDAHVLVIQVEGLRVVLTYSDLHRRRFAFFQSLLCEVGANWSEPQART
                     SAGLNFGATYYVGTATFDCADEEALCGVLEGIGARIVFLIDWNRARKRLEQFVGKTDA
                     VAILAEAARRRVGHMAWLAAGGEQLVFGAMQALGPEYFRIGDRLDGVLGTEKARAFVL
                     ESMALASAAMQQRQPLALVADDTRLLLARYLHRHDEFDLLAEHAAYCHALAEGLRDGL
                     AHGHERDRKAAREFSERAKDWERRADQLVMDARARAEARPRWEAFAELVETADDVADA
                     LEEAAFLLSLIAADHHQGWRGEVHQTMQLLADAVLGAAQDHVRALEIARGFGEDSSAE
                     DHEAFVAALWRVLNAERQCDVLLRDVRRALAEHVSDAATLNLSTDFAQALESATDWLL
                     ATGYGLRRLVFSRAGVGR"
     gene            3459..5198
                     /locus_tag="PKV97_08040"
     CDS             3459..5198
                     /locus_tag="PKV97_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB05865.1"
                     /translation="MVSNGRNGLPDGRAVYRHIAAIGEGLTARADPFGIAAPILHAQM
                     AWLMHPQELGERLAGLGTDLWQLQWHTWRRALGLPSADPVKPHADDPRFADPVWTDSA
                     TWDLVKEWYLAFTHHVQDMLYETPGLSGKERRRAAFWWRKWLNAVAPTNYLWSNPIAL
                     RRFIDTRGHSLWQGWQNMLADLQAGNVRMTDPDGFHVGDNLGTTPGAVIFRNRLLEVI
                     HYAPTQPQVHAEPVVIVTPWINKFYILDLNPRKSMVRYLLDQGLDVYITSWKNPDASM
                     REVRFDDYLVEGISAIVRVARECSGAERVHAVGYCIGGTALATWMAWAARHYGEDAVP
                     VRDWTLFTTLVDFHKPGDIEVFIDEESVAHLSEQMKEKGYLDGKDMAASFRLLRSNSL
                     IWHYVVHGWLYGESPAPFDVLYWNMDTTRMPAAMHAWYLRELYLNNRLVQPDALVVAG
                     EPIDLRRIRQPLYVVAAEDDHIAPWAQTFHTVHHVAADKRFVLSSSGHILGIVNPPVT
                     PPRRHYRVATAHRADRAPLWRERAALREGSWWEDWMAWLKPRAGELVAARPAADERHP
                     ALGDAPGTYVLEA"
     gene            5276..5485
                     /locus_tag="PKV97_08045"
     CDS             5276..5485
                     /locus_tag="PKV97_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05866.1"
                     /translation="MQTHAHLTAPGTPTGDRLGLHAGLESLGAQLTARWQSRTRKHVA
                     DLLDDADALADWHRNLERLRRGEHG"
     gene            5570..6001
                     /locus_tag="PKV97_08050"
     CDS             5570..6001
                     /locus_tag="PKV97_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05867.1"
                     /translation="MSTHTRSALRASLKQEDVALDERLPEIAGTRPPTRPETKAPPLP
                     AEPAKAKPEKRKRESFALVASDLRRLDALRSTMKAAGRPARKSALVRAGLAALAALDH
                     AETIALLDALPPLKRTAAKDDEKARAGKKKSSQRKAAARKR"
     gene            6031..7551
                     /locus_tag="PKV97_08055"
     CDS             6031..7551
                     /locus_tag="PKV97_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNB05868.1"
                     /translation="MDPDLGTELFESLGLDALLPESLSAWRPLLLEGMAFFLDRLPEA
                     RFGAIVAEQLALAASVSAAARATALLARCPTLHKLGQVVARQPGLDAELRRHLQALES
                     LPASTDDGALHARIRAELGAGHGLHLADRALAEGSVAVVLPFSWRQDGRSHEGVFKAL
                     KPGIGERLAEELAILPALGDFLERRGAEFGLPAIDYRSHLAAASRLLVQEIRLDREQA
                     NLRAAAALFAADADVFVPALLPWCTPTLTAMERIDGPKLADAALSAHESRRLGRALVG
                     GLLARPFWSCGEDAPFHGDLHGGNLLLAPDGRVAVLDWSLTARLSKAEREALVEVALA
                     ALALDASRIMAGLAGLGLRDTRAAPIGATVEHALDTLVRSGRPLGFDWLVGLLDALAL
                     QGAGGFGEQLAVFRKSWLSLAGVLGDLGAGHDTDLPLLGLGLQRFAAEWPARLLAEPD
                     SRAFSTHVSTADLLRAAADTWPAGLRYWLRLLGFAGAENRDARRADGKPTRLPQGL"
     gene            complement(7710..>8011)
                     /locus_tag="PKV97_08060"
     CDS             complement(7710..>8011)
                     /locus_tag="PKV97_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05869.1"
                     /translation="GSAAAEKRATATLLELAAPAAAGRRDKGEKVERDSFSMPAREHK
                     RIKALREALGEHGVLVSKSALLRAGLALLAERDTGEIARLVAALPKVAKGKRKRR"
BASE COUNT         1138 a         3029 c         2750 g         1094 t
ORIGIN      
        1 cggcgagcgc gcgcgcatcc agcgcggcgg tgcggacacg cgcggtttcc gcatcgatca
       61 cgccctcgtc gagcaggtcg agcgcgatcc gcgccgccgc ctgcggcgtg cgcttgccgg
      121 cacgcgtctg cagcaggtgg agcacgccct gctgcacggt gaactcgaag tcctgcatgt
      181 cgccgaaggc gcgctccagg cgggcggtca cgtcgagcac ggcctgccac accgcggggg
      241 cgaccgcggc gagctcgtcg tggccgtgcg cgctgcgccg gccgctgacc acgtcctcgc
      301 cctgcgcgtt gaagaggaaa tccacccatg gccgcggctc gccgctgctc gggtcgcgcg
      361 tgaagcccac gcccgcgccc gacagcccgc cggcattgcc gaacaccatg cgctgcacgg
      421 tgaccgcggt gcccatgtcg tcggccaggc cgtgcatcgc gcggtaggcg cgcgccttgt
      481 ccgcatgcca ggacgcgaac accgcggcga tggcctggcg cagctgctcg tgcgcatcct
      541 gcgggaaggc ctcgccggct tcgcgccggt aggtgtcgag gtggcgggcg gcgagctcgc
      601 gcagcgccgc gaagtcgagc acgcgctcgt cgccctggcc gcactcgcca cactcgccgc
      661 gcacggcctc gaggtcggcg tcgaaatgct gcgcgtcgat cccggcgacc acctcgccat
      721 agccggcgat caggcgccgg taggcatccc aggtcaggcg tggatggccg ctgacgcgca
      781 gcaggccgga cagcgtggtc tcggtgaggc cgatgtcgag cagggtctcc atcatccccg
      841 gcatcgacac cggcgcaccg gagcgcaccg aaagcagcag cggatggcgc gtgtcgccga
      901 ggcgcagccc gctcaggcgc tccagctcgg cgagcgccgg ccgccacgcg gcctcggcga
      961 gcgcgccggg ttcggcacac catccggtac cgatcacgaa ggccgggggc accggcaggc
     1021 cgagcgcggc catgcgcgcg aggttccacg ccttggagcc gagcgcctcg gcgctgcaat
     1081 cggcgggcag gggcgcgtcg gcgtggatca ggcggacgac acgacgggcg tcggccggcg
     1141 cggcggcgga cgccgcatcg ggatgcgcca tcatcggact ctcctcgttg tgttgcgcgc
     1201 tcatcgcccc acccccgcgc ggctgaagac cagccggcgc aggccgtagc cggtcgccag
     1261 cagccagtcg gtggccgact cgagcgcttg ggcgaagtcc gtgctcaggt tcagcgtcgc
     1321 cgcgtcgctg acgtgctcgg cgagcgcgcg gcgcacgtcg cgcagcagca cgtcgcactg
     1381 gcgctcggcg ttgagcaccc gccacagcgc ggcgacgaac gcctcgtggt cctcggcgct
     1441 gctgtcctcg ccgaatccgc gcgcaatctc gagcgcgcgc acgtggtcct gcgccgcacc
     1501 gagcaccgcg tcggcgagca gctgcatggt ctggtgcacc tcgccgcgcc agccctggtg
     1561 gtgatcggcg gcgatcagcg agagcaggaa ggcggcctcc tcgagcgcgt cggcgacgtc
     1621 gtcggcggtc tccaccagct cggcgaaggc ctcccagcgc ggtcgcgcct cggcgcgcgc
     1681 acgtgcgtcc atcaccagct ggtcggcgcg gcgctcccag tccttggcgc gctcggagaa
     1741 ttcgcgcgcc gccttgcggt cgcgctcgtg gccgtgggcg aggccgtcgc gcagaccctc
     1801 ggcgagcgca tggcagtagg ccgcgtgttc ggcgagcagg tcgaactcgt cgtgccggtg
     1861 caggtagcgc gcgagcagca ggcgggtgtc gtccgcgacc agcgcgagcg gctggcgctg
     1921 ctgcatcgcc gcgctcgcca gcgccatgct ctcgagcacg aaggcgcggg ccttctcggt
     1981 gccgagcacg ccgtcgaggc ggtcgccgat gcggaagtac tccggcccga gcgcctgcat
     2041 cgcgccgaac accagctgct cgccgccggc ggccagccag gccatgtgac cgacgcgccg
     2101 gcgtgcggcc tcggcgagga tcgccaccgc gtcggtcttg ccgacgaact gttcgagccg
     2161 cttgcgcgcg cggttccagt cgatgaggaa cacgatgcgc gcgccgatgc cctccagcac
     2221 cccgcacagc gcctcctcgt ccgcacagtc gaaggtcgcg gtgccgacgt aataagtggc
     2281 gccgaagttg agcccggcgc tggtgcgcgc ctgcggctcg ctccagttgg caccgacctc
     2341 gcacagcagc gactggaaga aggcgaagcg gcgacggtgg agatcggagt aggtgagcac
     2401 gacgcgcaga ccctcgacct gaatcaccag cacgtgggcg tcgttggtgc cgatgtcgtt
     2461 ctgcagcagc aggcgcgcac cgtcgcgggt ggcggcggtg tccagcccgg gatgatcgag
     2521 cttgagcgca cgggtggcgt tgagcccgcg catgaaggcg gcgatgcgcg agcgatcggc
     2581 cgcatccacc tgccacacgt gggcgccgtc gatggtctcg ctggagagct ccgaagcgag
     2641 ccggttgagc gccttgtgca ggtccatcac cagcaggtgc aggctgtcgt ccttgccgcg
     2701 cttgccgctg gtgagcgcgt ggagctgggc cgacgacagc gtgtccggcg cgagcgcttc
     2761 gagccaggcg caccagtgcg cacagcgcgc ggcgaagtcc gcgtcgcctt cgagcgggcg
     2821 cgccatggtg cacaggtcct cggcgagcag gccggccagc cggtcgagcc cggccacatg
     2881 cagctccttg ccctcgcggt aggcggtggc cggcagctgc gccagccagg gcgcatcgac
     2941 tccggcgacg gcgaactcgc gcgcgaggtc gagcgcgcgg gcgtcgccgc gctgcgcgca
     3001 gtcgtgcgcc gcctgcagca cgctgagata gagcttcagt cgatcgttgg cggccagcgc
     3061 cgcacgcacg cgcgccggcc tgagcagttc cgcctgtccc aggctggcga cggcctcgat
     3121 cttctccatg acatcctccc ggtattcgtg aaggattctg tgggtgaaat gtgacagtca
     3181 tgtcacgatg aatccgcacc ttgctgcgct gcgcaagaca cgcttcacga aagcgcaata
     3241 cggctgtcaa gcgccgggcg cagactatgt tcaacgacca gcgcgcgatc gtcgggcggg
     3301 gtagcggccc cctccgcacg ggatgcgcac ggaggcgatt tctcatgcca gcttcacgac
     3361 ggggcggcaa acggccggcg acggcccctt ctgccaagca gcaaccggac cggggcgcgc
     3421 acccggcccc gcccgactcg aacggccaca atggccgcat ggtctccaat ggccgcaacg
     3481 gcctccccga cggacgggcg gtttaccgcc acatcgccgc gatcggcgag ggcctgaccg
     3541 cacgcgccga ccccttcggc atcgccgcac ccatcctgca tgcgcagatg gcctggctga
     3601 tgcatccgca ggagctcggc gagcgcctcg ccggcctcgg caccgacctc tggcagctgc
     3661 agtggcacac ctggcggcgc gcgctcggcc tgcccagcgc cgacccggtc aagccgcacg
     3721 ccgacgatcc acgcttcgcc gaccccgtat ggaccgactc ggccacctgg gacctggtga
     3781 aggagtggta tctcgccttc acccaccacg tccaggacat gctctacgag acccccgggc
     3841 tgtccggcaa ggagcgccgg cgcgcggcgt tctggtggcg caagtggctc aacgcggtgg
     3901 cgccgaccaa ctatctctgg tccaacccga tcgcgctgcg acgcttcatc gacacccgcg
     3961 gccacagcct gtggcagggc tggcagaaca tgctcgccga cctgcaggcg ggcaacgtgc
     4021 gcatgacgga tcccgacggc ttccacgtcg gcgacaacct cggcaccacg ccgggcgcgg
     4081 tgatcttccg caaccgcctg ctcgaggtca tccactacgc gcccacgcag ccgcaggtgc
     4141 atgccgagcc ggtggtgatc gtcaccccct ggatcaacaa gttctacatc ctcgatctca
     4201 accccaggaa gagcatggtg cgctacctgc tcgaccaggg tctggacgtc tacatcacga
     4261 gctggaagaa ccccgacgca tcgatgcgcg aggtccgctt cgacgactac ctcgtcgagg
     4321 gcatctccgc gatcgtgcgg gtggcgcgcg aatgcagcgg cgccgagcgg gtgcacgcgg
     4381 tcggttactg catcggcggc accgcgctcg ccacctggat ggcctgggcg gcacgccatt
     4441 acggcgagga cgccgtgccg gtgcgcgact ggacgctgtt caccaccctg gtggacttcc
     4501 acaagccggg cgacatcgag gtcttcatcg acgaggagag cgtcgcccac ctgagcgaac
     4561 agatgaagga gaagggctac ctcgatggca aggacatggc cgcctccttc cgcctgctgc
     4621 gctcgaacag cctgatctgg cactacgtgg tgcacggctg gctctatggc gagtcgcccg
     4681 cgcccttcga cgtgctgtac tggaacatgg acaccacgcg catgcccgcc gcgatgcacg
     4741 cctggtacct gcgcgagctc tacctgaaca accgcctcgt ccagcccgac gccctcgtcg
     4801 tcgcgggcga gccgatcgac ctgcgccgca tccgccagcc gctttacgtg gtggccgcgg
     4861 aagacgacca catcgcgccg tgggcgcaga ccttccacac cgtgcaccac gtcgccgccg
     4921 acaagcgctt cgtgctgtcg agctccggcc acatcctcgg catcgtcaac ccgccggtga
     4981 cgccgcccag gcgccactac cgtgtcgcca ccgcccatcg cgccgaccgc gcgccactct
     5041 ggcgcgagcg cgccgcgctg cgcgaaggca gctggtggga ggactggatg gcctggctga
     5101 agccgcgcgc cggcgagctc gtagccgcac gccccgccgc cgacgaacgc catcccgcgc
     5161 tgggggatgc accggggacg tatgtgctgg aagcgtgagc cgcgaggggt cgagtgtgaa
     5221 ttgcgggcgc agcgcggatt ttttgcttgg tttcgattcg acaggaggaa gcatcatgca
     5281 gacgcacgct catctcaccg cccccggcac gccgacgggc gaccgcctcg gcctccatgc
     5341 aggcctcgag agcctcggtg cgcagctcac cgcgcgctgg caatcgcgaa cgaggaagca
     5401 cgtcgccgac ctgctcgacg acgccgacgc gctcgccgac tggcatcgca acctggagcg
     5461 cctgcgccgc ggcgagcacg gctgatctcc ttgcgcgcgc ccgtgcgcac gtgcatgagc
     5521 gcgcaagcgc agcctagaat gcggcgctga agcccccgag gagcgcaccg tgagcaccca
     5581 cacccgcagc gccctgcgcg ccagcctgaa gcaggaagat gtcgcgctcg acgagcgtct
     5641 gcccgagatc gccggaacac ggcccccaac ccgcccggaa accaaggccc cacccctgcc
     5701 ggcagagccc gccaaggcga aacccgagaa gcgcaagcgc gaatccttcg ctctggtcgc
     5761 cagcgacctg cgccgcctcg atgccctgcg cagcacgatg aaggccgccg gccggccggc
     5821 gcgcaagtcc gcgctggtgc gcgccggcct tgcggcgctc gcggccctcg accatgcgga
     5881 aacgatcgct ctgctcgatg cgctgccgcc gttgaagcgc accgccgcga aggatgatga
     5941 gaaggcccgg gccggcaaga agaaatcctc gcagcgcaag gctgcggccc gcaagcgctg
     6001 agcccggcgc gcttccgcag attctccgcc atggatcccg acctcggcac cgaactgttc
     6061 gagtcgctcg gcctcgacgc cctgctgccc gagtccctct ccgcctggcg tccgctgctg
     6121 ctcgagggaa tggccttctt cctcgatcgg ctgccggaag cccgcttcgg ggcgatcgtc
     6181 gccgagcagc tcgcgctcgc cgcgagcgtc tccgccgccg cgcgcgccac cgccctgcta
     6241 gcgcgctgtc ccacactgca caagctcggc caggtcgtcg cgcgccagcc cggcctcgac
     6301 gcggaactgc gccgccacct gcaggcgctc gaatccctcc ccgcgagcac ggacgacggc
     6361 gccctgcacg cccgcatccg cgccgagctc ggcgctggcc acggcctcca cctcgccgac
     6421 cgcgcgctcg ccgaaggcag cgtggccgtg gtgctgccct tctcgtggcg gcaggacggg
     6481 cgcagccatg agggcgtgtt caaggcgctc aagcccggca tcggcgaacg gctggccgag
     6541 gagctcgcca tcctgcccgc gctcggcgac ttcctcgaac gccggggggc ggagttcggc
     6601 ctgcccgcca tcgactaccg cagccacctc gccgccgcca gccgcctgct cgtgcaggag
     6661 atccgtctcg accgcgagca ggccaacctg cgcgccgccg cggcgctgtt cgccgccgac
     6721 gccgacgtct tcgtgccggc gctgctgccc tggtgcacgc ccacgctgac cgcgatggag
     6781 cgcatcgacg gccccaagct cgccgacgcc gcgctgtccg cgcacgaatc ccgccgcctc
     6841 ggccgcgccc tcgtcggcgg cctgctcgcc cgccccttct ggtcgtgcgg cgaggacgcg
     6901 cccttccatg gcgacctgca cggcggcaac ctgctgctcg cccccgacgg ccgcgtggcg
     6961 gtgctcgact ggagcctcac cgcgcgcctg agcaaggccg agcgcgaggc cctggtggaa
     7021 gtcgctctcg ccgctctcgc actcgacgca tcacgcatca tggccggcct cgccgggctg
     7081 ggcctgcgcg acacccgcgc cgccccgatc ggcgccaccg tcgagcacgc gctcgatacc
     7141 ctggtgcgca gcggccggcc gctcggcttc gactggctgg tcgggctgct cgatgcgctg
     7201 gcgctgcagg gcgcgggcgg cttcggcgag caactcgcgg tgttccgcaa gagctggctg
     7261 tcgctcgccg gcgtgctcgg cgacctcggc gccggtcacg acaccgacct gccgctgctc
     7321 ggcctcggcc tgcagcgctt cgccgccgag tggccggcac gcctgctcgc cgagcccgac
     7381 tcgcgcgcct tctccaccca cgtgtcgacc gccgacctgc tgcgcgcggc cgcggacacc
     7441 tggccggccg gactgcgcta ctggttgcgg ctgctcggat tcgcaggcgc cgaaaatcgc
     7501 gacgcgcgtc gcgccgacgg aaaaccgacg cggcttccgc agggcctgta ggagcgacgc
     7561 gagtcgcgat accgcgcttg cgcaggcgtg tgcgcaaatc cgctgcgctg cgaccaccgc
     7621 gctcccgccc gagcccaggc ccgaagtctc ccgggagccc ggttcgcgcc ttgcggcgct
     7681 gctccctggg caccatcgcg gttcccgcgt cagcgcctct tgcgcttgcc cttcgccacc
     7741 ttgggcaggg ccgcgaccag gcgggcgatc tcgccggtgt cgcgctcggc cagcagcgcg
     7801 aggccggcgc gcagcagcgc ggacttgctc acgagcacac cgtgctcgcc gagcgcctcg
     7861 cgcaacgcct tgatgcgctt gtgctcgcgc gccggcatcg agaacgaatc gcgctcgacc
     7921 ttctcgccct tgtcgcggcg cccggccgcg gcgggcgcgg caagctcgag cagcgttgcc
     7981 gtggcacgct tctccgccgc agcggatcca c
//
LOCUS       DAOQNN010000119         7931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4697_length_7931_cov_10.783647, whole genome shotgun sequence.
ACCESSION   DAOQNN010000119 DAOQNN010000000
VERSION     DAOQNN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4697_length_7931_cov_10.783647"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1702
                     /locus_tag="PKV97_08065"
     CDS             <1..1702
                     /locus_tag="PKV97_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB05870.1"
                     /translation="TRVSEIMVSGMASNTLEWQMFAHARPGTSDTSAIPTDQVNNPGT
                     INALINLAKRPTFANESVIKWRKTASCAKGVDETRCWCEPGRNGKCWEKSLHKEAVHH
                     ILKGGEDSIGALEAIQRVYFNIGSCSEQCWVNHLADLRQLDPQQRGFGQTPFDIGQCR
                     RDCPNFRAVEDRLGNILDFFMSAEGHATDLREARENTLRVANPGARYTDQDLIADLER
                     TFGAGAVARGREVFANGCARCHSSQPAAATGQLAGLDFHKLDLKTGLREDWLGNDGPT
                     AVSEVGTFRCRALHSNHMAGHVWQEYGSETLRAQPPDPNVKEPGDGGRGHYRNISLLN
                     LWAHAPFLHNNALGPELCGKPHNAANDFYAQRPRYVDASNVKLLPAERQPACFEYDPS
                     VEGRFKLYTLSMQALLNPAQRIPKVTLLNEDVTLRLGPRLWDGTDKERLLGFELTIPS
                     EIDGRGVTAGTLGNFQHKAFIVDLVRAKTAPDELERSLAKRLGKESARKVLADLQAVA
                     REVTGKPNGLVEALRARPYLIKQVYSSCTAEVENAGHRFGEDLSEADKNALIAFLATL
                     "
     gene            1720..2643
                     /locus_tag="PKV97_08070"
     CDS             1720..2643
                     /locus_tag="PKV97_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05871.1"
                     /translation="MTRISHWPFGALAVCMLAAMAVTGCGLFDDDGERAPEIAFAPHA
                     EGYAGKPDFAWLEHRHPLSTADLYKITPQNIAQLDQEQVDQIYARLSAGPIPDGAFDG
                     DLFFPRGGSGKLRLSEIAGGGLKGLLVNVAGGKLDLVGEMLWKGKVFYRDERLLRNRI
                     EDLTTLRKLGLVEESADTPLQKLNVGGKDQWLLFPAKLYCGQSLLDGRRESIIIDYAF
                     TDELPGYRRMPDLLAGRNGFAIRDEIRMVYPGFYLGRAYMDRAFVVNFVLHNAQMIAA
                     SREAFLKTGQVDQDCWSGTQQRLIAAAAGGG"
     gene            2715..4856
                     /locus_tag="PKV97_08075"
     CDS             2715..4856
                     /locus_tag="PKV97_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05872.1"
                     /translation="MRLSRPVSRHVLPVLLGALFGSVFMAPANAAEVDRAPSPHGQSL
                     FDRLLERGANGAQQVPFPFPALLQRVRAQLDGEDPNGGLAIVLIPLGRSLQRHAAGDV
                     DAFRFPRVVAAVTGEPASDAPAGHLYLKDRLYVAHHERAEALEVISYNEDAGRFEFQL
                     VLDYRAGATPRVVAANRTLCLACHQNEAPIFSRPSWDETSASPPVTARLAATGLDYQG
                     LPWRHGVDVPEAIDAATARANRLGVAQRLWRDGCALATPQAGIDCRAQALLHALRQRL
                     IGTGAPVARADATNRLVDPLLANWQARWPDGLVIPDPQIPNRQPFAGIEGGIAPPSDA
                     ELVRYADIPAVFDPLALRAPIEHWRGRDPGDVMRFIHLLGTLFSRADLARIERALARM
                     PQPAAREIDLSCTLRRATDATRLDLDCARGSEVRLQARIRLDGGRSADGILDRLELSG
                     SSAHTGVTLAIAKDHDPASGWPIRFALTAKAGGPIRASSGEAITILRLGGDDGSAHLP
                     GSPSAAEAVVAALELVDDLEPLGHAVEALAQANVAGQDDALTGGPARRMALLAPLLRG
                     LGEDVPAPPAAAAPPPTLMADVTRSRTTHWPADLQPFLRQCSQCHAENTAFPPGFLHG
                     DEAQVRANLATCAQRMLHRLDMNRLPPAARSKTPMPPPAAAHAGAFLQSPDLAAMRDL
                     LEHVLQASGVRSADVLAHPYATLAPCRVPTG"
     gene            4905..6452
                     /locus_tag="PKV97_08080"
     CDS             4905..6452
                     /locus_tag="PKV97_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB05873.1"
                     /translation="MNTPESVPTTHRLRLARTLLRILVLLPWLLLLLAGGYALVRFVP
                     DEPVVHADPVEHFKYGSTGGERASGFPYWIWQALPTVCAEHLPGPGYASLGLIFEEGR
                     DLPVGVSKRRHLGIDRVFLNCAACHAGLVRDTPAAPARLVVGMPAHRFDILAFQRFFF
                     ECAAGPTFSREFIVPEIERLSGGLGLVDRYLVYPVAIALMRERLLMLRARFGFVSAQA
                     PWGPGRVDTFNAAKVLFNFPVQRLPARERLGAADFPSIWNQRKRMQRDDGERMELHWD
                     GNNTHTEERNKSAAFGTGTTPPTIDLAAISRVEAWLLDLAPPAWPLPVDKTLAARGAA
                     LYAHYCADCHGASGSDFAGPKVGHVTPIADIATDPARLDSYTRDLAVNQATLYAGYPH
                     RFRHFRKTWGYANMPLDGIWLRAPYLHNGSVPTLRDLLEPAFARPTCFVRGGTLIDAV
                     RVGFASAADPAQCAAPTGRAQTATDHFLFDTTLPGNGNSGHEGPAYGTDLPAADKDAL
                     VEYLKTF"
     gene            6469..>7931
                     /locus_tag="PKV97_08085"
     CDS             6469..>7931
                     /locus_tag="PKV97_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05874.1"
                     /translation="MKFAPCTNPGPRPTRLLGRRRNTGRSLLVALLVLLALGAAGGAF
                     AWYKFFREEAQPEWITSDPDMRFKYGSIGAERDAGIPYWIFYVLPRMFPDKLPGPGGY
                     AAFGVVWEEGQELPVGFSKKVVGFPRVANNCASCHTTSYRTQADAAPTFVPTGPNHTL
                     NLWAFFRFLVDCAKDPRFNADNLMAEINLVTDLSFIDRLLYRFVIIPITRKRLLEREQ
                     QFAWLYRDDFPPWGRGRDDAMNLTKYFMIRWPMDDSFGPTDMPSLWNLGKYRADQGMR
                     MNFAGDSHDAYSVVIDSALGLLGAPPKDNAEFLGEVRWLIEYVSAKRAPPYPFAIDTA
                     AVARGKQVFDTTCAGCHASARTGTVIPLAEVGTSAERIATWNERAAREANQVVAGMGI
                     ERPGLVEAPLTGYVAAFLDGIWLRAPYLHNGSVPSLRDLLEPPAQRPTRFWRGYDVYD
                     PDRVGFVTHGPEAERIGTVHDVGARGGSNRGHAFGTTL"
BASE COUNT         1246 a         2911 c         2532 g         1242 t
ORIGIN      
        1 cacgcgcgtc tccgagatca tggtctcggg catggcatcg aacaccctcg aatggcagat
       61 gttcgcccat gccaggccgg gaaccagcga cacctcggcg atcccgaccg accaggtcaa
      121 caatcccggc acgatcaacg cactgatcaa tctcgccaag cgtccgacct tcgccaacga
      181 gtccgtgatc aagtggcgca agaccgcctc ctgcgccaag ggcgtcgacg agacccggtg
      241 ctggtgcgag ccgggccgca acggcaaatg ctgggagaag tccctgcaca aggaagccgt
      301 acaccacatc ctcaagggcg gtgaggactc gatcggcgcg ctcgaagcca tccagcgcgt
      361 gtacttcaac atcggctcgt gctcggagca gtgctgggtc aatcaccttg cagacctgcg
      421 ccagctcgat ccgcaacaac gcggctttgg ccagacgccc ttcgacatcg gccagtgtag
      481 gcgggactgc ccgaacttcc gcgccgtcga ggaccgcttg ggaaacattc tcgacttctt
      541 catgtcggcc gaaggccatg ccaccgacct gcgcgaggca cgcgagaaca cgctgcgggt
      601 tgccaacccc ggcgcgcgtt acaccgatca ggacctgatc gccgatctcg aacgcacgtt
      661 cggagcgggt gcggtcgcgc gtggccgtga agtgttcgcc aatggctgcg cacgctgcca
      721 ctcgagtcag cccgcggccg cgactggcca gcttgccggc ctcgatttcc acaagctcga
      781 cctcaagacg gggctgcgcg aggactggct gggcaacgat ggccccaccg cagtgtccga
      841 ggtcggcacc ttccgctgcc gcgccctgca ctcgaaccac atggccggcc acgtctggca
      901 ggaatacggc tccgaaaccc tgcgcgcaca gccgcccgac cccaacgtca aggaacccgg
      961 cgacggtggc cgcggccact accgcaacat ctcgctgctc aacctgtggg cgcacgcccc
     1021 cttcctgcac aacaacgcgc tcggccccga gctttgcggc aaaccgcaca acgccgccaa
     1081 cgacttctat gcccagcgtc cgcgctacgt cgatgcgagc aacgtcaagc tgttgcccgc
     1141 cgagcgccag cccgcctgct tcgagtacga cccgagcgtc gaaggccgat tcaagctgta
     1201 caccctgtcg atgcaggcgc tgctcaatcc cgcccagcgc atccccaagg tgacgctgct
     1261 gaacgaggac gtgaccctgc gcctcggccc gcggctatgg gacggcaccg acaaggaacg
     1321 cctgctcggt tttgaactga cgatcccgag cgagatcgac ggccgcggcg tgaccgccgg
     1381 cacgctgggc aacttccagc acaaggcctt catcgtcgat ctcgtgcgcg cgaagaccgc
     1441 gcccgacgag ctcgagcgca gcctcgccaa gcgcctgggc aaggagagcg cgaggaaggt
     1501 gcttgccgac ctccaggccg tcgcgcggga agtgaccggt aagccgaacg gcctggtcga
     1561 ggcgctgcgc gcgcgcccct acctgatcaa gcaggtgtac agcagttgca ccgccgaggt
     1621 cgagaacgcg ggccaccgct ttggcgagga cttgtccgag gccgacaaga acgccctgat
     1681 cgccttcctc gcaacgcttt gagcatgggg gacttcatca tgacaaggat ctcgcactgg
     1741 ccgtttgggg cgctcgccgt ctgcatgctg gccgccatgg cggttacggg atgcggcctg
     1801 ttcgacgacg acggggagcg ggcgccggag atcgccttcg ccccgcatgc cgaaggctat
     1861 gccggcaagc ccgatttcgc ctggctggaa catcgccatc cgctgtcgac ggccgacctg
     1921 tacaagatca cgccgcagaa catcgcgcag ctcgaccagg agcaggtcga ccagatctat
     1981 gcgcgcctga gcgccgggcc gatccccgac ggcgcgttcg acggcgacct gttcttcccg
     2041 cgcggtggca gcggcaagct gcgcctgtcc gagatcgccg gcggcggcct gaagggcttg
     2101 ctggtcaatg tggcgggcgg caagctcgac ctcgttggcg agatgttgtg gaagggcaag
     2161 gtgttctacc gcgacgagcg cctgctgcgt aaccgcatcg aggatctcac caccttgcgc
     2221 aagctcggcc tcgtcgagga aagcgcggac accccgctgc agaagctcaa tgtcggcggc
     2281 aaggaccagt ggctgctgtt cccggcgaag ctctactgcg gccagagcct gctcgacggc
     2341 cggcgcgaat ccatcatcat cgactacgcc ttcaccgatg aactgccggg ctaccggcgc
     2401 atgcccgacc tgctcgcggg ccgcaacggc ttcgcgatcc gcgacgagat ccgcatggtc
     2461 tacccgggct tctaccttgg tcgggcctac atggaccgcg ccttcgtggt gaacttcgtc
     2521 ctccacaacg cgcagatgat cgcggcgagt cgcgaagcgt tcctgaagac cggccaggtc
     2581 gatcaggatt gctggagcgg cacccagcag cgcttgatcg ccgccgcggc agggggtggc
     2641 tgagaacggt ccgcgcgtgg ttttgcgggc ggcggctgcg tccgcggccc gcaagatcgc
     2701 gcaggggaac gagcatgaga ctgtctcgtc ctgtctcccg ccatgtgctc ccggtgctgc
     2761 tgggcgcgct gttcggcagc gtgttcatgg ctccggccaa tgccgccgag gtcgaccgcg
     2821 caccgtcgcc gcacgggcag tcgctgttcg atcgcctgct cgagcgcggg gcgaacggcg
     2881 cgcagcaggt gcccttcccg ttccccgcac tgctccaacg cgtgcgcgca cagctcgatg
     2941 gcgaggaccc gaacggcgga ctggcgatcg tgctgatccc gctcgggcgc tcgctgcagc
     3001 gccacgccgc aggagacgtc gatgccttcc gttttccgcg tgtggtggcc gcggtcaccg
     3061 gtgagccggc aagcgacgca cccgcgggac acctctacct gaaggaccgc ttgtacgtcg
     3121 cgcaccacga acgtgccgag gcgctcgagg tgatcagcta caacgaggat gcgggtcgct
     3181 tcgagttcca gctcgtgctc gactaccgcg ccggggcaac gccgcgggtg gtcgccgcga
     3241 accgtacgct ctgcctcgct tgccaccaga acgaggcgcc gatcttctcg cgcccgtcgt
     3301 gggacgagac cagcgccagc ccgccggtta ccgcacgcct ggctgcaacc ggcctcgact
     3361 atcagggtct gccctggcgt cacggcgtcg atgtgcctga agcgatcgac gccgcgaccg
     3421 cacgcgcgaa tcgcctcggc gtggcccagc gcctgtggcg ggacggctgc gccctcgcca
     3481 ccccgcaggc cggcatcgac tgccgtgccc aggcgctgct gcacgcgctg cgccagcggc
     3541 tgatcggcac cggggcgccg gtcgcgcgcg cggatgccac gaaccgcctg gtggatccgc
     3601 tgctcgccaa ctggcaggcg cgttggccgg acggcctcgt cattcccgat ccgcaaatcc
     3661 ccaaccgcca gcccttcgcc ggcatcgagg gcgggattgc gcccccgagc gatgccgagc
     3721 tcgtgcgcta tgcggacatt ccggcggtct tcgatccgct cgcgctgcgg gcgccgatcg
     3781 agcactggcg cggacgcgac ccgggcgacg tgatgcgctt catccacctg ctcgggacgc
     3841 tgttctcgcg cgccgacctt gcgcgcatcg agcgcgccct cgcgcgcatg ccacagcctg
     3901 cagcgcggga gatcgacctg agctgcaccc tgcgccgggc caccgacgca acgcggctcg
     3961 acctcgactg cgcgcgcgga tccgaggtcc ggctgcaggc gcgcatcagg ctcgacggcg
     4021 ggcgcagcgc cgacggcatc ctcgaccgtc tcgagttgtc cgggtcgagc gcgcacaccg
     4081 gtgtgacgct ggccatcgcg aaggaccacg acccggcatc cggctggccg atccgcttcg
     4141 cgctcactgc caaggcgggt ggtccgatcc gcgccagcag tggggaagcc atcaccatct
     4201 tgcggctcgg tggcgacgac ggcagcgccc acctgccggg gtcgccgagc gcggccgaag
     4261 cggtcgtggc agccctcgaa ctcgtcgatg acctcgagcc gctcgggcac gccgtcgagg
     4321 ccctcgcgca ggcaaatgtc gccggtcagg acgatgcgct gacgggcggc ccggcacgcc
     4381 ggatggccct gctcgcgccg ctgctacgcg ggctcggcga ggacgttcct gccccgcccg
     4441 ccgcggcagc gcctccaccc acgttgatgg ccgatgtcac ccggtcccgc acgacgcact
     4501 ggcccgccga cctgcagccc ttcctgcgcc agtgcagcca gtgtcatgcc gagaacaccg
     4561 ccttcccgcc cggcttcctg cacggtgacg aagcacaggt acgcgccaat ctcgcgacct
     4621 gtgcgcaacg catgctccat cggctggaca tgaaccggct gccgcccgca gcccgctcga
     4681 aaaccccgat gccgccgccc gctgcagcgc atgccggcgc cttcctgcag tcacccgacc
     4741 tggcggcgat gcgcgatctg ctcgagcacg tgctgcaggc aagtggcgta cgcagcgccg
     4801 acgtgcttgc ccatccgtac gccaccctcg cgccctgccg agtcccgaca gggtgatcgg
     4861 cgctccgaca tcgcaccgca ccgtcctgca gatccgaggt caccgtgaac acgcccgagt
     4921 ccgtccccac aacgcatcgc ctgcgcctcg cgcgcacgct gctccgcatc ctcgtgctgc
     4981 tgccctggct gctgctcctg ctcgcgggcg gctatgccct cgtgcggttc gtgccggacg
     5041 agccggtcgt ccatgcagac ccggtggagc acttcaagta cggctcgacc ggcggcgagc
     5101 gcgcatcggg cttcccgtac tggatctggc aggccttgcc gaccgtgtgc gcagaacatc
     5161 tgcccggtcc cggctacgcg tcgctcggcc tgattttcga ggaaggccgc gacctgccgg
     5221 tgggcgtgtc gaagcggcgc cacctcggca tcgatcgcgt cttcctcaac tgcgccgctt
     5281 gccacgccgg cctcgttcgc gacacgcccg ctgcgcccgc acgcctggtc gtcggcatgc
     5341 ccgcccatcg cttcgacatt ctcgccttcc agcgcttctt cttcgaatgc gccgctggcc
     5401 cgaccttctc gcgtgagttc atcgtgccgg agatcgagcg cctgagcggc ggactcgggc
     5461 tcgtcgatcg ctacctcgtc tatccggtgg cgatcgcgtt gatgcgcgag cgcctgctga
     5521 tgctgcgcgc gcgctttggc ttcgtgtccg cgcaagcgcc ctgggggccg ggtcgggtcg
     5581 acaccttcaa cgccgcaaag gtcctgttca attttccggt gcaacgactt cctgcccgcg
     5641 agcgcctcgg cgccgccgat tttccgtcga tctggaacca gcgcaagcgc atgcaacgtg
     5701 acgatggcga gcgcatggaa ctgcactggg acgggaacaa cacccacacc gaggagcgca
     5761 acaagagtgc agccttcggt accggcacca cgccccccac catcgacctg gcggcaatct
     5821 cccgggtcga ggcctggctg ctcgacctcg ccccgccggc gtggccgctg ccggtggata
     5881 agacactcgc cgcgcgcggc gcggcgctgt atgcgcacta ttgtgccgat tgccacggcg
     5941 cgagcggcag cgacttcgca gggccgaagg tcggccatgt caccccgatc gcggacatcg
     6001 ccaccgaccc cgcccggctc gattcctaca cccgggatct ggcggtcaat caggcgaccc
     6061 tctatgcggg ctatccgcat cgtttccgcc atttccgcaa gacctggggc tacgccaaca
     6121 tgccgctcga cggcatctgg ctgcgcgcgc cctacctgca caacggctcc gtgcccaccc
     6181 tgcgcgatct gctcgagccc gccttcgctc ggccgacgtg ctttgtgcgc ggcggcacgc
     6241 tgatcgatgc ggtgcgggtc ggcttcgcca gcgctgccga tcctgcgcaa tgcgccgcgc
     6301 cgacgggcag ggcccagacc gcaaccgatc acttcctgtt cgacaccacg ctgccgggca
     6361 acggcaacag cggtcacgaa ggcccggcct atggcaccga tctgcccgcc gcggacaagg
     6421 atgcgctggt cgagtacctg aagaccttct gatgcgagga gtgccgccat gaaattcgcc
     6481 ccctgcacga atccggggcc gcgccccacc cggctcctcg gccggcgccg caacaccggc
     6541 cgcagcctgc tcgttgcgct gctcgtgctg ctcgcgctcg gcgccgccgg cggcgccttc
     6601 gcctggtaca agttctttcg cgaggaagcg cagccggaat ggatcaccag cgatcccgac
     6661 atgcgcttca agtacggctc gatcggtgcc gaacgcgatg ccggcatccc ctactggatc
     6721 ttctacgtgc tgccgcggat gtttcccgac aagctgccgg gcccgggcgg ctacgccgcc
     6781 ttcggcgtgg tgtgggaaga aggccaggag ctgccggtcg gcttctcgaa gaaggtggtg
     6841 ggctttccgc gcgtggccaa caactgcgcg tcctgccaca ccacgagcta ccgcacccag
     6901 gccgatgcgg cgccgacttt cgtgccgacc ggccccaatc acacgctgaa cctgtgggcc
     6961 ttcttccgct ttctggtcga ctgcgcgaaa gacccacgct tcaacgccga caacctgatg
     7021 gccgagatca atctggtcac cgacctgtcc ttcatcgacc gcctgctcta tcgcttcgtg
     7081 atcatcccga tcacgcgcaa gcgcctgctc gagcgcgaac agcagttcgc ctggctgtac
     7141 cgcgacgatt tcccgccctg gggccgcggc cgcgacgatg cgatgaacct gaccaagtac
     7201 ttcatgatcc gctggccgat ggacgacagt ttcggcccga ccgacatgcc ttcgctgtgg
     7261 aacctcggca agtaccgcgc cgatcagggc atgcgcatga acttcgccgg cgacagccac
     7321 gacgcgtatt cggtggtgat cgactccgcg ctcggcctgc tcggcgcgcc acccaaggac
     7381 aacgccgagt tcctcggcga ggtgcgctgg ctcatcgaat acgtttcggc gaaacgtgcg
     7441 ccgccgtatc cgttcgcaat cgataccgcc gctgtagcgc gcggcaagca ggtgttcgac
     7501 acgacctgcg ccggctgcca cgcatcggcg cgcaccggaa cggtgatccc gcttgccgag
     7561 gtcggcacca gcgcggagcg catcgcgacc tggaacgaac gcgcggcgcg cgaagcgaat
     7621 caggtggtgg ccgggatggg catcgagcgc ccgggcctgg tcgaagcccc gctgaccggc
     7681 tacgtcgccg ccttcctcga cggcatctgg ctgcgcgcgc cctacctgca caacggctcg
     7741 gtgccctcgc tgcgcgacct gctcgaaccg cccgcgcagc gccccacccg cttctggcgc
     7801 ggttatgacg tctatgatcc ggaccgggtc ggcttcgtca cccacggccc cgaggccgaa
     7861 cgcatcggca ccgtgcacga cgtcggggcc cgtggtggca gcaaccgtgg tcacgcgttc
     7921 ggaaccactc t
//
LOCUS       DAOQNN010000120         7899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4723_length_7899_cov_11.110275, whole genome shotgun sequence.
ACCESSION   DAOQNN010000120 DAOQNN010000000
VERSION     DAOQNN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4723_length_7899_cov_11.110275"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="PKV97_08090"
     CDS             complement(<1..110)
                     /locus_tag="PKV97_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNB05875.1"
                     /translation="MLDNLTQRLSKVVKTLRGQARLTEENIQEAMREVRMA"
     gene            159..989
                     /gene="ccsA"
                     /locus_tag="PKV97_08095"
     CDS             159..989
                     /gene="ccsA"
                     /locus_tag="PKV97_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNB05876.1"
                     /translation="MRTILLHLLPASLYAGLGVFFWRTCMTRGSALEPRRECMSTRER
                     LLLLGAIVAHGAALHLAIFPGSEMRFGFGVAVSLMVWLAVCFYWVETLYTRLDGLHAA
                     VMPAGAIGSLVPMLFPGDHVLANATSPAFRAHFVVAMLAYSLFTLAALHAMLMSVAGR
                     QLHNARFSRLLSGLPPLLTMEALLFRLISIAFVLLTLTLASGVLFSESLFGQAFRVDH
                     KTVFAFVSWFLFGGLLVGRRLWGWRGRLALRWTLAGFVALMLAYVGSRFVIEVVLHRT
                     "
     gene            1073..2353
                     /locus_tag="PKV97_08100"
     CDS             1073..2353
                     /locus_tag="PKV97_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HNB05877.1"
                     /translation="MTDLTLTLQIVALVVLLTLSACFSMSETVMMAANRYRLRSLANQ
                     GSRGATLALDLLARTDRLLGVILLFNNLVNTGAATLVSVITLELFGNESWILGVATLL
                     ATFAILVFSEITPKVVGANHADRLAPFVAYPLTGLLRASYFAVWFINLFSRGLLNLMR
                     LQPKEETSSALSVEELRAMVIESGQYIPHKHRSMLLNLFDLESITVEDVMTPRGAIES
                     IDLSDPEDIVRRQIATSFHTRLAVYDGDSEHVIGVLHQRKLLGNTLEENFSVDRIRDL
                     LARPYFVPADTPLYSQIQFFQENQQRLGFVVDEYGEILGLITLEDIIEELIGKFTTST
                     PDVGDRMHWGEDGSVLVDGTSNLRELNRRLELALPIDGPKTLNGLILEHLQDIPETGV
                     SMKIADTPIEVVQTQDRMVRTARIFRPRIEGAGQ"
     gene            2442..4157
                     /gene="pilB"
                     /locus_tag="PKV97_08105"
     CDS             2442..4157
                     /gene="pilB"
                     /locus_tag="PKV97_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HNB05878.1"
                     /translation="MAATSKPALSGFARALIQHGHLKEADAIACTTHAGEAATAFILE
                     VANRGLMSCAAMARFAAETFGYPLLDLAAFDAAMFAREAVDRKLLAKHQVAPLAKRQS
                     RITLATADPSNLRALDEIRFQTGLQIELVIVEADKLKRAVEALSESAADTLKELTGEA
                     FDMDLLQQDGPAQPRDEEEANEVDDAPVVKFIQKVLIDAINEGASDVHFEPYEKFYRI
                     RVRTDGILRDIAQPPLVLKDKIAARIKVISRLDISEKRVPQDGRMKLVLSRNKAIDFR
                     VSTLPTLHGEKIVMRILDPSSAMLGIDALGYDPEQREALLAAVERPYGMILVTGPTGS
                     GKTVSLYTCLNLLNKPGVNISTAEDPAEINLAGINQVNVNEKAGLTFSAALRAFLRQD
                     PDVIMVGEIRDIETAEIAIKAAQTGHLVMSTLHTNDAPSTLERLKNMGVAPFNIASSV
                     ILITAQRLARRLCSCKKPVDIPAQALLEAGFEPDELDGGWQPMGPGGCERCKGSGYKG
                     RVGIYQVMPISEEIARIIMTGGNSMDIAAQARSEGVLDLRQSGLRKVRQGITSLEEVL
                     ATTNE"
     gene            4173..5402
                     /locus_tag="PKV97_08110"
     CDS             4173..5402
                     /locus_tag="PKV97_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNB05879.1"
                     /translation="MATASRAARATGPREDLYTWEGKDKTGKVVRGEIRATGDAMVQA
                     MLRRQGIQVIKIRKQKMARGGRISEKDIALFTRQLATMMKSGVPLLQAFDIAMKGSGN
                     AALVRLLNDIRTDVETGSNLSQAFAKHPAHFDRLFCNLVAAGEQAGILDGLLDRIATY
                     KEKILAIKSKIKSALFYPAAVVVVAGLVISVMMLFVIPEFKSVFSSFGAELPVPTLMV
                     IAISDAFVAYWYLIFGALIAALVAIAWSYKRSTAMQIAVDRLVLRIPVIGAIIRKATV
                     ARWTRTLSTMFAAGVPLVEALDSVGGASGNHVYVVATHQIQSEVSTGTSLTVAMQNAE
                     VFPTMVVQMVSIGEESGQLDAMLGKVADFFEQEVDDAVAGLSQLLEPLIMVFLGTIIG
                     GLVVAMYLPIFKLGSVV"
     gene            5407..6258
                     /locus_tag="PKV97_08115"
     CDS             5407..6258
                     /locus_tag="PKV97_08115"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNB05880.1"
                     /translation="MIELLRDPLAFTILSGLVGLFVGSFLNVVIHRLPRMMERDWHAQ
                     AAELRGEQAPANERFNLATPRSRCPHCGHQITMLENIPIVSYVVLRGRCAHCGAGISR
                     RYPIVEALSALLSAYAAWHFGFGASALGALLFVWAMVALTFIDLDTQLLPDDITLPLL
                     WLGLAFNLGTTFAELPDAVIGAMAGYLALWAVFWLFKLATGKEGMGYGDFKLLAAIGA
                     WLGWQMLPLTILLSSLVGAVVGILLIVVARHGRNVPIPFGPYLAAAGLIALFWGERIT
                     ERYLGLL"
     gene            6416..6679
                     /locus_tag="PKV97_08120"
     CDS             6416..6679
                     /locus_tag="PKV97_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNB05881.1"
                     /translation="MPIYEYRCPSCGFQKEHLQKMSDEPLSVCPSCGTTGYAKQLSAA
                     GFQLKGSGWYATDFKGGAKSAAPAAESTPSPATGGCGGGCACH"
     gene            6762..7388
                     /locus_tag="PKV97_08125"
     CDS             6762..7388
                     /locus_tag="PKV97_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNB05882.1"
                     /translation="MKKYFITGLLIWLPLAITYMVIAWIVGTLDAILLWLPAEYQPSR
                     YIGFDIPGVGVVASLLLVFFTGLVAANVLGQKLVQLWEALLARIPVVKSIYYSVKQVS
                     DTVFSSNGQAFRKALLVQYPREGVWTIAFLTGQPGGDAAEHLRGDYVSVYVPTTPNPT
                     SGFFLMMRRSEVVELDMSVDDALKYIISMGVVAPPARRPEPVPALSDT"
     gene            7476..>7899
                     /locus_tag="PKV97_08130"
     CDS             7476..>7899
                     /locus_tag="PKV97_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNB05883.1"
                     /translation="MRTHYCGKVTAADLDQIVTICGWVHRRRDHGGVIFIDLRDREGL
                     VQVVCDPDRAEMFKIAESVRNEFVLKMSGKVRRRPAGTENANLTSGEIEILCHDIEVL
                     NAAATPPFQLDDENLSETVRLTNRVIDLRRPQMQKNLML"
BASE COUNT         1263 a         2847 c         2460 g         1329 t
ORIGIN      
        1 gccatgcgca cctcgcgcat cgcctcctgg atgttttctt ccgtcaggcg ggcctggccg
       61 cggagggtct tgacgacctt ggacaggcgt tgggtcagat tgtcgagcat gttggggcag
      121 gccttgggcg cgtgacttgg agtaaactgc ggctacgtat gcgcacaatt ctacttcatc
      181 tcctccccgc ctcgctttac gccggtctgg gcgtgttctt ctggcgcacc tgcatgacgc
      241 gcggttccgc gctggagccg cgccgggagt gcatgagcac gcgcgagcgc ctgctgctgc
      301 tgggcgccat cgtcgcgcac ggcgcggcgc tgcacctggc gatcttcccc ggcagcgaga
      361 tgcgcttcgg cttcggcgtc gcggtctcgc tgatggtctg gctggcggtg tgcttctact
      421 gggtcgagac cctctacacc cggctcgacg gcctgcacgc tgcggtgatg cccgcaggcg
      481 cgatcggcag cctggtgccg atgctgttcc cgggcgacca cgtgctcgcc aacgccacct
      541 cgccggcctt ccgtgcccac ttcgtcgtgg ccatgctcgc ctacagcctg ttcaccctgg
      601 ccgccctgca cgcgatgctg atgtcggtgg ccgggcgcca gctccacaac gcacgcttca
      661 gccgcctgct ctccggcctg ccgcccctgc tcaccatgga ggcgttgctg ttccggctga
      721 tttccatcgc cttcgtgctg ctcacgctga cgctggcgag cggcgtgctg ttctccgaga
      781 gcctgttcgg ccaggccttc cgggtcgacc acaagacggt gttcgccttc gtctcctggt
      841 tcctcttcgg cggcctgctg gtgggccgcc gcctgtgggg ctggcgcggc cggctggcgc
      901 tgcgctggac cctggccggc ttcgtcgcgc tgatgctcgc ctacgtgggc agccgcttcg
      961 tcatcgaagt cgtgctgcat cgcacctgag tccgcccgcc gaagtccgcg tgcattgaca
     1021 ccgtcacgag gggctccaat actcggggcc cctttccttt ccaccgcccc tcgtgacgga
     1081 cctcaccctg acgcttcaga tcgtcgccct cgtcgtgctg ctgaccctgt cggcatgctt
     1141 ctcgatgtcg gagacggtga tgatggcggc caaccgctac cgcctgcgct ccctcgccaa
     1201 tcagggcagc cggggcgcca ccctggccct cgacctgctc gcccgcacgg accggctgct
     1261 cggcgtcatc ctgctgttca acaacctggt caacacgggc gccgccaccc tggtcagcgt
     1321 gatcacgctc gagctcttcg gcaacgagag ctggatcctc ggcgtggcga ccctgctcgc
     1381 caccttcgcg atcctggtgt tctccgagat cacgcccaag gtcgtcggcg ccaaccacgc
     1441 cgatcgtctc gcccccttcg tggcctaccc actcaccggc ctgctgcgcg cgtcgtattt
     1501 cgcggtatgg ttcatcaacc ttttctcgcg cgggctgctg aacctgatgc gtctgcagcc
     1561 gaaggaggag acgagctcgg cgctctccgt cgaagagttg cgcgcgatgg tgatcgagtc
     1621 gggccagtac atcccgcaca agcaccgcag catgctgctc aacctcttcg acctcgagag
     1681 catcacggtc gaggacgtga tgacgccgcg cggagcgatc gagagcatcg acctgtccga
     1741 ccccgaggac atcgtccgcc gccagatcgc caccagcttc cacacccggc tggcggtcta
     1801 cgacggcgac agcgagcacg tgatcggcgt gctgcaccag cgcaagctgc tcggcaacac
     1861 gctggaggaa aatttcagcg tggaccgcat ccgcgacctc ctggcccgcc cctatttcgt
     1921 gccggccgac acgccgctct actcgcagat ccagttcttc caggagaacc agcagcgtct
     1981 cggcttcgtg gtggacgaat acggcgagat cctcggcctg atcaccctcg aggacatcat
     2041 cgaggaactc atcggcaagt tcaccaccag caccccggac gtgggcgacc gcatgcactg
     2101 gggcgaagac ggcagcgtgc tggtcgatgg cacgtccaac ctgcgcgagc tcaaccgccg
     2161 gctcgagctc gcactcccga tcgatggtcc gaagacgctc aacggtctga tcctcgaaca
     2221 cctgcaggac atcccggaga ccggggtgtc gatgaagatc gccgataccc cgatcgaggt
     2281 cgtgcagacc caggaccgca tggtgcgcac cgcgcgcatc ttccgtccgc ggatcgaggg
     2341 cgccggacag tgagcccgcg cgccccggca ccggctgcgc gcatctttac aaggccgggg
     2401 gcggcgggca acatcggccg cagtccggac acgagcaagg catggcggcg acctccaaac
     2461 cggccctgag cggcttcgcc agggccctga tccagcatgg ccacctcaag gaggcggacg
     2521 cgatcgcatg caccacccat gcgggcgagg ccgcgaccgc cttcatcctc gaggtcgcca
     2581 accgcggcct gatgagctgc gcggcgatgg cccgcttcgc cgccgagacc ttcggctatc
     2641 cgctcctcga cctcgccgcc ttcgatgcgg cgatgttcgc gcgcgaggcg gtcgaccgca
     2701 agctgctcgc caagcaccag gtcgcgccgc tcgccaagcg ccagagccgc atcacgctcg
     2761 ccaccgccga cccttccaac ctgcgcgcgc tggacgagat ccgcttccag accggcctgc
     2821 agatcgagct ggtgatcgtc gaggcggaca agctcaagcg cgcggtcgag gccctgtccg
     2881 agtcggccgc agacacgctc aaggagctca ccggcgaagc cttcgacatg gacctgctgc
     2941 agcaggacgg tcccgcgcag ccgcgcgacg aggaggaagc caacgaggtc gatgacgccc
     3001 cggtcgtcaa gttcatccag aaggtgctga tcgacgcgat caacgagggc gcctcggacg
     3061 tccacttcga gccctacgag aagttctacc gcatccgcgt gcgcaccgac ggcatcctgc
     3121 gcgacatcgc ccagccgccg ctggtgctca aggacaagat cgccgcgcgc atcaaggtga
     3181 tctcgcgcct cgacatctcg gagaagcgcg tgccgcagga cgggcgcatg aagctcgtgt
     3241 tgtcgcgcaa caaggcgatc gacttccgcg tctcgacgct gccgacactg cacggcgaga
     3301 agatcgtgat gcgcatcctc gatccgagct cggcgatgct cggcatcgac gcgctcggct
     3361 acgaccccga gcagcgcgag gcgctgctcg ccgcggtcga gcgcccctac ggcatgatcc
     3421 tggtcaccgg ccccaccggc tcgggcaaga cggtgtcgct gtacacctgc ctcaacctgc
     3481 tcaacaagcc gggcgtgaac atctccaccg ccgaggaccc tgccgagatc aatctcgccg
     3541 gcatcaacca ggtcaacgtc aacgagaagg ccggcctcac cttctccgcc gcgctgcgcg
     3601 ccttcctgcg ccaggacccg gacgtgatca tggtgggcga gatccgcgac atcgagaccg
     3661 ccgagatcgc catcaaggcc gcgcagaccg gccacctggt gatgtccacc ctgcacacca
     3721 acgacgcgcc ctccacgctc gagcgcctca agaacatggg cgtggcgccg ttcaatatcg
     3781 catcctcggt gatcctgatc acggcccagc gcctcgcccg ccggctatgc tcctgcaaga
     3841 agcccgtcga cattcccgcc caggccctgc tggaggccgg cttcgagccc gacgagctcg
     3901 acggcggctg gcagccgatg ggaccgggcg gttgcgaacg ctgcaagggc agcggctaca
     3961 agggccgggt gggcatctac caggtcatgc cgatcagcga ggagatcgcc cgcatcatca
     4021 tgaccggcgg aaactccatg gacatcgccg cccaggcccg cagcgagggc gtgctcgacc
     4081 tgcgccagtc gggcctgcgc aaggtgcgcc aaggcatcac ctcgctcgaa gaagtgctgg
     4141 cgacgaccaa cgaataaagg acgctcgatt tcatggccac tgcaagccgc gcggcacgtg
     4201 ccaccggtcc gagggaggac ctttatacct gggaaggcaa ggacaagacc ggcaaggtcg
     4261 tccgcggcga gatccgcgcc accggcgacg ccatggtcca ggccatgctg cgccgccagg
     4321 gcatccaggt gatcaagatc cgcaagcaga agatggcgcg cggcggacgc atctccgaaa
     4381 aggacatcgc gctgttcacc cgccagctcg ccacgatgat gaaatccggt gtgccgctgc
     4441 tgcaggcctt cgacatcgca atgaaaggct ccggcaacgc cgcgctcgtg cgcctgctca
     4501 acgacatccg caccgacgtc gagaccggct ccaacctgtc gcaggccttc gccaagcacc
     4561 ctgcgcactt cgaccggctg ttctgcaacc tggtcgcagc gggcgagcag gcgggcatcc
     4621 tcgacggcct gctcgaccgc atcgccacct acaaggaaaa gatcctcgcg atcaagagca
     4681 agatcaagtc ggcgctgttc tatcctgccg cggtggtcgt ggtggcgggt ctcgtgatct
     4741 cggtgatgat gctgttcgtg atccccgagt tcaagagcgt gttctccagc ttcggtgccg
     4801 agctgccggt gcccaccttg atggtcatcg ccatctcgga cgccttcgtg gcctactggt
     4861 acctgatctt cggcgccctc atcgcggccc tcgtggcgat cgcctggagc tacaagcgct
     4921 cgactgcgat gcagatcgcg gtcgaccggc tggtgctgcg catcccggtg atcggcgcga
     4981 tcatccgcaa ggccacggtc gcacgctgga cgcgcacgct gtcgaccatg ttcgcggccg
     5041 gcgtgccgct ggtcgaggcg ctcgactcgg tgggaggcgc atcgggcaac cacgtctatg
     5101 tggtggcgac acatcagatc cagagcgagg tcagcaccgg caccagcctg acggtcgcga
     5161 tgcagaacgc ggaagtcttt cccaccatgg tggtgcagat ggtgtcgatc ggcgaggagt
     5221 ccggccagct cgacgccatg ctgggcaagg tcgccgattt cttcgagcag gaggtggacg
     5281 acgcggtcgc cggcctgtcg caactgctcg agccgctgat catggtcttc ctcggcacca
     5341 tcatcggcgg cctggtggtg gcgatgtacc tgccgatctt caagctcggt tcggtcgtct
     5401 gaacccatga tcgagcttct ccgcgacccg ctcgccttca ccatcctctc cggcctggtc
     5461 ggactcttcg tcggcagctt cctcaacgtc gtgatccatc ggctgcccag gatgatggag
     5521 cgcgactggc acgcccaggc ggccgagctg cgtggcgagc aagcacccgc caacgagcgc
     5581 ttcaacctcg ccacgccgcg ctcgcgctgc ccgcactgcg ggcaccagat cacgatgctg
     5641 gagaacatcc cgatcgtcag ctacgtggtg ctgcgcgggc gctgcgcaca ttgcggcgcc
     5701 ggcatcagca ggcgctaccc gatcgtcgaa gccctctccg cgctgctgtc ggcctacgcc
     5761 gcctggcatt tcggcttcgg cgccagcgcg ctcggggccc tgctgttcgt gtgggcgatg
     5821 gtcgcgctca ccttcatcga cctcgacacc cagctgctgc ccgacgacat caccctgccg
     5881 ctgctgtggc tgggcctcgc cttcaacctc ggcaccacct tcgccgagct tcccgacgcg
     5941 gtgatcggcg cgatggcggg ctacctcgcg ctgtgggcgg tgttctggct gttcaagctc
     6001 gccaccggca aggagggcat gggctacgga gacttcaagc tgctcgccgc gatcggcgcc
     6061 tggctgggct ggcagatgct gccgctgacc atcctgctgt cctcgctggt gggcgcggtg
     6121 gtcggcatcc tgctgatcgt cgtcgcccgc cacggccgca acgtgccgat ccccttcggc
     6181 ccctacctcg ccgcggccgg cctgatcgcg ctgttctggg gcgagcgcat caccgagcgc
     6241 tacctcgggc tgctctgagc gcgcgccgcg cgcgccgctg cggacggtcc gccgcggggg
     6301 gcttgaagcc ctcctccccc gccctcatct tgcacccggg cgcgaccgtt ccggacgcgc
     6361 tttttcctta tgctgcgttg cgttagactt gcgcacttgt tttcgggagc acgccatgcc
     6421 catctatgaa tatcgttgcc cgagctgtgg tttccagaag gaacacctgc aaaaaatgag
     6481 cgacgagccc ctgtccgtct gcccgtcctg cggcacgacc ggctacgcca agcagttgtc
     6541 ggcggccggc ttccaactca agggctcggg ctggtacgcc accgacttca agggcggagc
     6601 caagagcgcg gccccggcgg ccgaatccac gccctcgcct gcgaccggcg gctgcggcgg
     6661 cggctgcgcc tgccactgag cggaccgacc catctcgcgg cgggccggcc gcggcgcggc
     6721 agacgcaagc agccgcgcag cgcggcggcc aggaacccca cttgaaaaaa tacttcatca
     6781 ccggcctgct gatctggctc ccgctggcga tcacctacat ggtgatcgcc tggatcgtcg
     6841 gcacgctcga cgcgatcctg ctgtggctgc ccgccgaata ccagccgagc cgctacatcg
     6901 gcttcgacat ccccggcgtg ggcgtcgtcg cgagcctgct gctggtgttc ttcaccggcc
     6961 tggtcgccgc caacgtgctc gggcagaaac tcgtgcaact ctgggaagca ctgcttgccc
     7021 gcatcccggt cgtcaagtcg atctactaca gcgtcaagca ggtctccgac acggtgttct
     7081 cgagcaacgg ccaggccttc cgcaaggcgc tgctggtgca atacccgcgc gagggcgtgt
     7141 ggaccatcgc cttcctcacc ggccagccgg gcggcgacgc ggccgagcac ctgcgcggcg
     7201 actacgtcag cgtgtatgta ccgaccacgc ccaacccgac ctcgggcttc ttcctgatga
     7261 tgcggcgcag cgaggtcgtc gagctcgaca tgagcgtgga cgacgcgctc aagtacatca
     7321 tctcgatggg cgtggtcgct ccgccggcgc gcaggccgga acccgtgccc gcgctgtccg
     7381 acacctgaac gaagctcccc gaacactcca gcctctccac cctgccgaat cctgaatccc
     7441 cgggcgcaag cgcccccgaa cctgcggaat ccgtcatgcg aactcactac tgcggcaaag
     7501 tcaccgctgc cgacctcgac cagatcgtca ctatttgcgg ctgggtgcat cgccgccgcg
     7561 accacggcgg cgtcatcttc atcgacctgc gcgaccgcga gggcctggtg caggtggtgt
     7621 gcgaccccga ccgcgccgag atgttcaaga tcgccgaatc cgtgcgcaac gagttcgtgc
     7681 tcaagatgag cggcaaggtg cgccgccgtc cggccggcac cgagaacgcc aacctgacct
     7741 cgggcgagat cgagatcctc tgccacgaca tcgaggtgct caacgccgcc gccacaccgc
     7801 ccttccagct cgacgacgag aacctctccg agaccgtgcg cctgaccaac cgcgtcatcg
     7861 acctgcgtcg cccgcagatg cagaagaacc tgatgctgc
//
LOCUS       DAOQNN010000121         7883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4738_length_7883_cov_9.283214, whole genome shotgun sequence.
ACCESSION   DAOQNN010000121 DAOQNN010000000
VERSION     DAOQNN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4738_length_7883_cov_9.283214"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKV97_08135"
     CDS             complement(<1..626)
                     /locus_tag="PKV97_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HNB05884.1"
                     /translation="MRTTSLRSRCDHLRDDLAGLSPAYFGMAMATGIVSLSAHMQAWT
                     WLASALFWLNASLYAGLWCLLLTRIAWHPGAVRADLLDHLRAPGFFTTVAATGVVGTQ
                     CLLIADSLRWAIALWCVALVLWLLLTYTIFTMLVVKEDKPPLDRGINGGWLLAVVATQ
                     AVAVLGTLIATRIPQPYRLELNFFALSMWLWGGMLYIWMMSLIFYRYT"
     gene            complement(822..2033)
                     /locus_tag="PKV97_08140"
     CDS             complement(822..2033)
                     /locus_tag="PKV97_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNB05885.1"
                     /translation="MADDAPDHVHDLLIVGAGPVGLALALALKDAGLDIVLADARARE
                     AVARDPRDLALAHGTRLTLQRLGVWDGLPTTAIQHIHISHQGGLGRTLIDAAEHELPA
                     LGYVASAGTLATALRKAVDAAGIPVFDETEITNLAAGEDDVIASLAGTGRPAPTLRAR
                     LAACAEGGLRAGDPNVVEHDYGQHALIADVQVAGGHRHTAFERFTPQGPVALLPKGEG
                     YALVHVARPETADELLALDDAAYLARLQAHIGGRARLTGVGPRLRYPLVLRYRRSTIA
                     QRTVWLGNAAQTLHPVAGQGFNLALRDVWALSEALRRATEAARRDSADTAPNPFDAGA
                     AAILASYANARGLDRLGTIRFTDTLVRVFSNDFAPLRHARGAALFALDLLPPLRNFVA
                     RRMMFGARAWP"
     gene            complement(2026..3369)
                     /locus_tag="PKV97_08145"
     CDS             complement(2026..3369)
                     /locus_tag="PKV97_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNB05886.1"
                     /translation="MNAPGTAPMDTTPFRARRARLLQRMQTAGGGVAILPTAPERVRN
                     RDAHYAYRHDSYFYYLTAFREPEAVVVLVAGKETKQILFCREKNEEREIWDGYRWGPD
                     AARAAFGFDEAWTIGDLEKRLPDYLADQPLLWTSLGYDNDWDARVLGALNAVRDKART
                     GLTPPHSVRDLRAELDEMRLVKDASELATMRQAAQISAAAHCRAMRATRPGRHEYEIE
                     AELLHAFRAAGSQAPAYTSIVAGGANACVLHYVDNDQRLNDGDLLLIDAGCELDGYAS
                     DITRTFPVSGRFSSPQRAVYELVLAAQAAAREATRPGAHWNQPHDAAVKVLAQGMLDL
                     GLLQGSLDGVLENGDYRRFYMHRTGHWLGMDVHDAGEYKLGGEWRPLVEGMVLTIEPG
                     CYIRAAEDVPEAFWNIGIRIEDDAIVTADGCALITEDAPKAVADIEALMRDARHG"
     gene            complement(3413..4759)
                     /locus_tag="PKV97_08150"
     CDS             complement(3413..4759)
                     /locus_tag="PKV97_08150"
                     /inference="COORDINATES: protein motif:HMM:NF017835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HNB05887.1"
                     /translation="MTLPHRFLASLALAAMLSAGAPGTSAADPEMTIGAPLKILPATP
                     PTGAVVLFSDADGWDGPERGAAEALAKQGLVVIGIDLEGVMRRMRARHDDCSILIGEI
                     EAVSHELQRQTGAHAYHFPVLAGVGEGGTMALAVADQAAAATLAGAVAVDPLPAAALR
                     RALCGHDGRPTDGTARLMPPPLPFSVELIASTDAGQAAHTLVARLAAGRHDVQVTDSP
                     EKPAAALLAGVGRAGHPRQDDALGDLPLIELPAPGPGTGAFAILYSGDGGWRDLDKDV
                     AALLQREGLPVVGVDVLRYFWSQRSPEQGARDLARIIDAYARRWKATRVVLIGYSFGA
                     DVLPALYNRLPADMKGRVAQISLLGFATRASFEVTVSEWLGHAREDSVPTLPEALRID
                     QRLLQCFQGDEDDEAACTGLGAGAEIIRTSGGHHFDGDYEALARRILHGVERRSPH"
     gene            complement(4756..7341)
                     /gene="mprF"
                     /locus_tag="PKV97_08155"
     CDS             complement(4756..7341)
                     /gene="mprF"
                     /locus_tag="PKV97_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HNB05888.1"
                     /translation="MNTDKAKRLIETVLDQARRIRPAVVPALAVLVLALAWTAVSHLV
                     GEVSYAEVAAALAATPDRALAIALGCTLLSFVALMVYDLGALDFIDRKVPRNVVALTS
                     FCAYSVGNTVGFGPLTAGAIRYRFYTPYGLEPDEVARIVAFVTAAFAIGLAATAAIGL
                     LVAADELAMLSVAPFVLQLIGGGMLAAFVALCLAAGEGRTVSLFGSPARLPSWRQMVR
                     QLVATIVDIGASATVLWVLLPAGSIDLPAFVAIYAVAVGLGVVSHVPAGLGVFETVIV
                     AALSGRLPIDQILSALILYRVIYHLVPLILAVLLITGLELRKAARTPLMAAALKAGHR
                     LAPPLIGAFTLVLGAVLVFSGVTPASEEALDVLSDVVPLPLLEGAHFLGSVLGMALLI
                     IARGLVYRLDGAWWLAVIVVPLSAILAFFKGLALGEALMLTALFAALLASRRAFSRHA
                     SLLHQAMAPGWLAAMGVLLVTAVAILLFVYKDVDYSHSLWWQFEFYSEAPRSLRALIG
                     ITLTAGLGAWWTLMKPVGVRSALPDEEALERARRIVAAQAHVDAGLVAMGDKRLLFSE
                     DGRAFIMYAIQGRSWVALSDPVGPRETWPELVWNFAELARHAGGRAVFYQVAPESLGL
                     YADAGLMAFKLGEEARVRLSEFDLKGGKRASLRQSLNRAERMGLSFEILAGPALDACM
                     DELSALSGAWLAQHKAREKRFSLGAFDPAYVRRQPVALLRQAGRVVAFANVLLTDLKQ
                     EATVDMMRFSADAPNGAMEALLLHLILHFQREGYEWFLLGMAPLSGLSASAAAPIWHR
                     VGRTVFEHGERFYNFSGLRAFKAKFLPEWQPRYLAVAGGINPMLALADITVLISGGLK
                     GVIGK"
     gene            complement(7338..>7883)
                     /locus_tag="PKV97_08160"
     CDS             complement(7338..>7883)
                     /locus_tag="PKV97_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNB05889.1"
                     /translation="INHAHLGHRLEEALGDGTAFGVRIAWSPEASALETAGGIRHALP
                     LLGNTPFVVVNGDVFCDADFTSLRAAAEGLHADGPLAHLLLVDNPEHHPEGDFHLDAA
                     GLLHADGAPRLTFSGLGAYHPALFAALADDTPAKLAPLLRAAMNAGQVTGRHHAGRWI
                     DVGTPRRLADLDCELQHKRPS"
BASE COUNT         1172 a         2639 c         3014 g         1058 t
ORIGIN      
        1 aaggtgtagc ggtagaagat cagcgacatc atccagatgt acagcatgcc gccccacagc
       61 cacatcgaca gcgcgaagaa gttgagctcc agccggtagg gctgcggaat gcgcgtcgcg
      121 atcagggtgc ccagcaccgc gaccgcctgc gtggcgacga ccgcgagcag ccagccgccg
      181 ttgatgccgc ggtcgagggg aggcttgtct tccttgacca ccagcatggt gaagatcgtg
      241 taggtcagca gcagccagag caccagtgcg acgcaccaga gcgcgatcgc ccagcgcagg
      301 ctgtcggcga tcagcaggca ctgggtgccg accacgccgg tcgcggcgac ggtcgtgaag
      361 aagcccggag cgcgcagatg gtcgagcaga tcggcacgca ccgcgccggg gtgccaggcg
      421 atccgggtca gcagcaggca ccacaggccg gcatagaggc tggcgttgag ccagaacaag
      481 gcgctcgcga gccaggtcca ggcctgcatg tgggcggaaa gcgagacgat gccggtcgcc
      541 atcgccatgc cgaagtaggc cggcgagagt ccggccaggt cgtcgcgcag gtggtcgcag
      601 cgactgcgca gggacgtcgt gcgcacggat ggcatcgctc agccgggcgt cgtcggcagc
      661 gcgccggctg cccgccggtc gccgcatcca tgccgccaag ctcgattccg gtccatcgcc
      721 tccctcccga ttgccgggcc gacgggtccc gcacgtttca gtctagaccg tggaatccga
      781 cccgccacgt ccgcggcgtc ttcgggcgcg cggcaggcgg ctcacggcca ggcgcgcgcc
      841 ccgaacatca tccggcgcgc gacgaagttg cgcagcggcg gcagcaggtc cagggcgaag
      901 agggccgcgc cgcgggcgtg acgcaagggg gcgaagtcgt tgctgaacac ccgcaccagg
      961 gtgtcggtga aacggatcgt gccgaggcgg tccaggccgc gagcgttcgc atagctcgcg
     1021 aggatggcag cggcgccggc gtcgaagggg ttcggtgcag tgtcggctga atcccgccgc
     1081 gcagcctcgg tcgcacgccg cagcgcttcg gacagcgccc acacatcccg cagcgcgagg
     1141 ttgaagccct gtcccgcgac cgggtgcagg gtctgcgccg cgttgccgag ccacaccgtg
     1201 cgctgggcga tggtcgagcg ccggtagcgc agcaccaatg gatagcgcag gcgcgggccg
     1261 acgccggtca gccgcgcgcg gccgccgatg tgggcctgca ggcgggcgag gtaggcggca
     1321 tcgtcgagcg ccagcagttc gtcggccgtc tcggggcggg cgacatgcac cagcgcgtag
     1381 ccctcgccct tgggcagcag cgccaccgga ccctgcggcg tgaagcgctc gaaggcggtg
     1441 tggcgatggc cgcccgcgac ctgcacgtcg gcgatgagcg cgtgctggcc gtagtcgtgc
     1501 tcgaccacgt tggggtcgcc cgcgcgcagg ccaccctcgg cgcaggccgc caggcgggcg
     1561 cgcagcgtgg gggcggggcg gccggtgccc gcgaggctgg cgatcacgtc gtcctcgccc
     1621 gcggccaggt tggtaatctc ggtctcgtcg aacaccggga tgccggccgc gtctaccgcc
     1681 ttgcgcaggg ccgtggcgag cgtgcctgcc gaggcgacgt agccgagcgc gggcagctcg
     1741 tgctcggcgg cgtcgatcag ggtgcggccg agcccgccct ggtgcgagat gtggatgtgc
     1801 tggatcgccg tggtcggcag cccatcccaa acgccgaggc gctgcagcgt gagccgggtg
     1861 ccgtgggcga gcgcgaggtc gcgcgggtcg cgcgccaccg cctcgcgcgc gcgcgcgtcg
     1921 gcgagcacga tgtcgagccc ggcgtccttc aatgccagcg cgagcgccag gccgaccggg
     1981 ccggcgccga cgatcagcag gtcatggaca tggtcgggcg cgtcgtcagc catgacgggc
     2041 gtcccgcatc agggcctcga tgtccgcaac cgccttgggc gcgtcctcgg tgatcagcgc
     2101 gcagccgtcg gcggtgacga tcgcgtcgtc ctcgatgcgg atgccgatgt tccagaaggc
     2161 ctcgggcacg tcctcggccg cgcggatgta gcagcccggc tcgatggtca acaccatgcc
     2221 ctcgaccagc ggccgccact cgccgccgag cttgtattcg ccggcgtcgt gcacgtccat
     2281 gcccagccag tggccggtgc ggtgcatgta gaagcggcga tagtcgccgt tctctagcac
     2341 gccgtccagg ctgccctgga ggagaccgag gtcgagcatg ccctgggcga gcaccttcac
     2401 cgcggcgtcg tgcggctggt tccagtgcgc gccggggcgg gtggcctcgc gcgccgcggc
     2461 ctgggcggcg agcacgagct catagaccgc gcgctgcggg ctggagaagc gaccgctcac
     2521 cgggaaggtc cgggtgatgt cggaggcgta gccgtcgagc tcgcagccgg cgtcgatcag
     2581 caacaggtcg ccgtcattga ggcgctggtc gttgtcgacg tagtgcagca cgcaggcgtt
     2641 ggcgccgccg gcgacgatgc tggtgtaggc gggggcctgg ctgccggcgg cgcggaaggc
     2701 gtgcagcagc tcggcctcga tctcgtattc gtgccggccc gggcgtgtgg cgcgcatcgc
     2761 gcggcagtgg gcggcggcgg agatctgtgc tgcctggcgc atggtggcga gctcggacgc
     2821 gtccttgacc aggcgcatct cgtcgagctc ggcgcgcagg tcgcgcaccg agtgcggcgg
     2881 ggtgaggccg gtgcgggcct tgtcgcgcac ggcgttgagc gcgccgagta cgcgcgcgtc
     2941 ccagtcgttg tcgtagccga ggctggtcca cagcaggggc tggtcggcga ggtagtcggg
     3001 gaggcgtttt tcgaggtcgc cgatggtcca cgcctcgtcg aagccgaagg ccgcgcgcgc
     3061 cgcgtcgggg ccccagcggt agccgtccca gatctcgcgt tcctcgttct tctcgcggca
     3121 gaagaggatc tgcttcgtct ccttgcccgc caccagcacg accacggcct cgggttcgcg
     3181 gaaggcggtg aggtagtaga agtagctgtc gtggcggtag gcgtagtggg cgtcgcggtt
     3241 gcgcacgcgc tcgggggcgg tgggcaggat ggcgacgccg ccgccggcgg tctgcatgcg
     3301 ctgcagcagg cgggcgcggc gggcgcggaa gggagtggtg tccatggggg cggtgccggg
     3361 tgcgttcatg ggcggaatta tgtcacgcgg cgacgccgcg ggccgcttgc gcctaatgcg
     3421 gcgaacgccg ctccacgccg tgcaggatgc ggcgggcgag ggcttcgtag tcgccgtcga
     3481 aatggtgtcc gccgctggtg cggatgatct ccgccccggc gcccaggccc gtgcaagcgg
     3541 cctcgtcgtc ttcgtcgccc tggaagcact gcagcaggcg ctgatcgatg cgcagcgctt
     3601 cgggcagggt cggcacgctg tcctcgcgcg cgtggccgag ccattcggag accgtgacct
     3661 cgaagctcgc gcgggttgcg aacccgagca gcgagatctg cgcgacgcga cccttcatgt
     3721 cggccggcag gcggttgtag agcgcgggaa ggacgtccgc gccgaaggag tagccgatca
     3781 gcacgacgcg cgtggccttc caccgcctgg cgtaggcgtc gatgatgcgg gcgagatcgc
     3841 gcgcgccctg ttcgggcgag cgctgcgacc agaagtagcg cagcacgtcc acgcctacca
     3901 ccggcaagcc ctcgcgttgc agcagcgcgg cgacgtcctt gtcgaggtcc cgccagccgc
     3961 cgtcgcccga gtagaggatg gcgaaggcgc ccgtgcccgg gccgggtgcg gggagttcga
     4021 tcagcggcag gtcgccgagc gcgtcgtcct ggcgtggatg cccggcgcga ccgactccgg
     4081 ccagcagcgc ggcggcgggc ttttcggggc tgtcggtgac ctgcacgtcg tgccgccccg
     4141 ccgcgagccg ggcgacgagc gtgtgtgcgg cttggcccgc gtccgtgctg gcgatcaatt
     4201 ccaccgagaa gggcaggggc ggcggcatca gccttgcggt gccgtcggtc ggccggccgt
     4261 cgtggccgca cagtgcccgg cgcagtgcgg ctgcgggcag cgggtcgacc gcgaccgctc
     4321 cggcgagggt cgcggccgca gcctggtcgg cgaccgcgag cgccatcgtc ccgccttcgc
     4381 cgacccccgc cagcaccggg aaatggtagg catgggcgcc cgtctggcgc tggagttcgt
     4441 ggctgaccgc ctcgatctcg ccgatcagga tgctgcagtc gtcgtggcgc gcccgcatgc
     4501 gtcgcatcac gccttccagg tcgatgccga tcaccacgag gccctgcttc gccagcgcct
     4561 ccgccgcgcc gcgctcgggg ccgtcccagc cgtccgcgtc cgagaacagc accacggcgc
     4621 cggtgggcgg cgtggcgggg aggatcttca gcggcgcgcc gatcgtcatc tcgggatccg
     4681 cggcggacgt gcccggcgcg cctgcggaca gcatggcggc cagggcgagg gacgcgagga
     4741 atcgatgggg cagggtcatt tgccgatcac tcccttgagc ccgccgctga tcagcacggt
     4801 gatgtccgcc agcgcgagca tggggttgat gccacccgcc accgcaagat agcggggctg
     4861 ccactcgggc aggaacttgg ccttgaaggc gcgcaggccg ctgaagttgt agaagcgttc
     4921 gccgtgctcg aacaccgtcc tgccgacgcg gtgccagatc ggcgcggccg cgctcgccga
     4981 caggccggac agcggtgcca tgccgagcag gaaccattcg tagccttcgc gctggaagtg
     5041 caggatcagg tgcagcagca gcgcctccat cgcgccgttg ggcgcgtcgg cggaaaagcg
     5101 catcatgtcg acggtggcct cctgcttgag gtcggtgagc agcacgttgg cgaaggccac
     5161 gacgcgccct gcctggcgca gcagggcgac gggctggcgg cgcacgtagg ccgggtcgaa
     5221 ggcgccgagc gagaagcgct tctcgcgcgc cttgtgctgc gcgagccacg cgcccgagag
     5281 tgcggagagc tcgtccatgc aggcgtccag cgccggtccg gcaaggatct cgaagctcag
     5341 gcccatgcgc tcggcgcggt tgagcgattg tcgcaggctg gcgcgcttgc cgcccttgag
     5401 gtcgaactcg gagagtcgga cgcgcgcctc ctcgccgagc ttgaacgcca tcaggccggc
     5461 atccgcgtag aggccgagcg attcgggggc gacctggtag aagaccgcac gaccgccggc
     5521 atgacgggcc agttcggcga agttccacac cagctccggc caggtctcgc gcgggccgac
     5581 cggatcggac agggcgaccc aggaacgccc ctggatcgcg tacatgatga aggcgcggcc
     5641 atcctcggaa aacagcagac gcttgtcgcc catggcgacc aggcccgcgt cgacgtgggc
     5701 ttgggcggcg acgatccggc gggcgcgctc gagcgcttct tcgtccggca gggccgagcg
     5761 caccccgacc ggcttcatca gcgtccacca tgcgcccagg ccggcggtga gcgtgatgcc
     5821 gatcagcgcc cgcaggctgc gcggtgcctc gctgtagaac tcgaactgcc accacagcga
     5881 gtggctgtaa tccacgtcct tgtagacgaa cagcaggatc gcgaccgccg tcaccagcag
     5941 cacgcccatc gcggcgagcc agcccggcgc catcgcctga tggagtagcg aggcgtggcg
     6001 cgagaaggcg cgccgcgagg cgagcagggc cgcgaacagg gccgtcaaca tgagggcctc
     6061 gccgagggcc aggcccttga agaaggccag gatggccgac agcggaacga cgatcaccgc
     6121 cagccaccag gcgccgtcga ggcggtacac cagcccgcgc gctatgatca gcaaggccat
     6181 gccgagcacg ctgccgagga agtgggcgcc ttcgagcagc ggcagcggca cgacgtcgct
     6241 cagcacgtcc agggcctcct cgctcgccgg ggtgaccccc gagaacacca gcaccgcgcc
     6301 gagcaccagg gtgaatgcgc cgatcaaggg gggcgccagg cggtgcccgg cctttagggc
     6361 cgcggccatc agcggcgtcc gggccgcctt gcgcagctcc agcccagtga tcagcagcac
     6421 cgccaggatc agcggcacca ggtggtagat cacgcggtag aggatgagtg cgctcaggat
     6481 ctggtcgatc ggcaggcgtc cgctcagcgc ggcgacgatc accgtctcga acacccccag
     6541 gccggccggc acgtggctca ccacgccgag gccgacggcg accgcgtaga tcgcgacgaa
     6601 ggccggcagg tcgatgctgc cggcgggcag cagcacccac agcacggtgg ccgaggcgcc
     6661 gatgtcgacg atggtcgcga ccagctggcg gaccatctgg cgccacgagg gcaggcgcgc
     6721 aggagagccg aacaggctca ccgtcctgcc ctctccagcg gccaggcaga gcgccacgaa
     6781 ggcggccagc atgccgccgc cgatcagctg cagcacgaac ggcgcgaccg acagcatggc
     6841 cagttcatcg gcggcgacga gcaggccgat ggccgccgtt gccgccagcc cgatcgcgaa
     6901 ggcggcggtg acgaaggcga cgatgcgggc gacctcgtcg ggctctaggc cgtagggcgt
     6961 gtagaagcgg tagcggatcg cgccggcggt cagcggcccg aagccgaccg tgttgcccac
     7021 cgagtaggcg cagaacgagg tcagcgcaac gacgttgcgc gggaccttgc ggtcgatgaa
     7081 gtcgagcgcg ccgaggtcgt acaccatcag ggcgacgaag ctgagcaggg tgcagccgag
     7141 cgcgatggcg agcgcccggt ccggcgtcgc ggcgagtgcc gccgcgacct cggcatacga
     7201 cacctcgccg acgaggtggc tgacggccgt ccaagcgagc gcgaggacga gcacggccag
     7261 ggcggggacc acggccgggc ggatgcgtct ggcttgatcg agcacggtct cgatcaggcg
     7321 cttggcttta tcggtgttca agagggcctc ttgtgttgga gctcgcagtc gaggtccgcc
     7381 aggcgccgcg gggtgccaac gtcgatccag cgcccggcgt ggtgccggcc ggtgacctgg
     7441 cccgcgttca tcgccgcgcg cagcaggggg gcgagtttgg cgggggtgtc gtcggcgagg
     7501 gcggcgaaca gggcgggatg gtaggcgccg aggccggaga aggtgagccg tggggcgcca
     7561 tcggcatgga gcaggccggc ggcatcgagg tggaagtcac cttccgggtg gtgctcgggg
     7621 ttgtccacca gcagcaggtg ggcgagcggg ccgtcggcgt gcaggccttc ggcggcggcg
     7681 cgcagggacg tgaagtcggc gtcgcagaac acgtcgccat tgaccacgac gaagggggtg
     7741 ttgccgagca gcggcagggc gtggcggatg ccgccggcgg tctccagtgc gctggcctcg
     7801 ggcgaccacg cgatgcggac gccgaaggcc gtgccgtcgc cgagcgcttc ttcgagccgg
     7861 tggccgaggt gggcgtggtt gat
//
LOCUS       DAOQNN010000122         7873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4748_length_7873_cov_10.628422, whole genome shotgun sequence.
ACCESSION   DAOQNN010000122 DAOQNN010000000
VERSION     DAOQNN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4748_length_7873_cov_10.628422"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PKV97_08165"
     CDS             complement(<1..659)
                     /locus_tag="PKV97_08165"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HNB05890.1"
                     /translation="MSMKHPIIAVTGSSGAGTTTVKHTFEAIFHRENVNAAIVEGDSF
                     HRHTRAEMKTLIEEAERAGLRGISHFGPEANLLSELESLFRAYGEAATGRRRYYIHNE
                     AEAMRWNLPLGTFTEWEDLPVGTDCLFYEGLHGALVTDEADIASHVDLLIGVVPIINL
                     EWIQKLHRDTKLRGYTAEAVQDTILRRMHDYVHYIVPQFARTHINFQRVPVVDTSNPF
                     IA"
     gene            complement(730..1539)
                     /locus_tag="PKV97_08170"
     CDS             complement(730..1539)
                     /locus_tag="PKV97_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HNB05891.1"
                     /translation="MPTAAHRLARARALESMVRDIARAEILPRYLKSARNRKADGSLF
                     TEADMASQQRFSEALPAFLPGAVLGEEMTAAEQAALWKEGARGLWCIDPIDGTTNFAN
                     GIPLFAVSIAYLIDHEPVIGVVYNPITDESFYAARGAGAFLNGTELPLRAGATELRDA
                     VAGVDFKRISHHLGDELAVRPPYYSQRNFGSSALEWCFVAAGRLDVYLHGGQMLWDYA
                     AGRLILAEAGGIAEALDGGVLMSGPAIKRGVIAATSPALFAEWSAWVKARS"
     gene            1610..2332
                     /locus_tag="PKV97_08175"
     CDS             1610..2332
                     /locus_tag="PKV97_08175"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNB05892.1"
                     /translation="MISRFHFPDRLPAGGEVALPEALAHHALRVLRLRDGEAVVLFDG
                     SGGEVDARLAVRGKAVFARLGEARAVERESPLRIVLVQALASGDKMDWVVQKAVELGV
                     HAIQPVQAERSVLRLAAERADKRVAHWQQVAVAACEQCGRNRVPEIRPLQPLAHWLGT
                     HRAAPGYVLAPGGAKGFADEPEPKDEVHLLVGPEGGWSEGEMAAFDAAGCRRVRLGPR
                     VLRTETAGLAAVAALQARWGDY"
     gene            complement(2360..5020)
                     /locus_tag="PKV97_08180"
     CDS             complement(2360..5020)
                     /locus_tag="PKV97_08180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB05893.1"
                     /translation="MAGSHALSTAFEPSSIAVIGASDAPGSVGRVIFRNLVEGGYRGT
                     LYAVNPKYGSIQDHPCHRSVEDIGRPVDLAIIATPARMLHSVTAQCGRSGVRNAVIIS
                     ASGNALERRIVETARETRLRLIGPGSLGIARPGSGINAALTRIAVKPGEIALISQSGA
                     MCSVVLDWALTNDVGFSSVISLGGTVDIDYGETLEYLIHDTQTRYILLHVDHIRNARQ
                     FMSALRSAARVKPIILLKAGRHGRSDNNAADADNAGLADVVFDAAMRRAGVVRVQNID
                     QLFFAAKALSCGFRPREDSLAIVSNGSGPADLAADRAKDLGTGLVGLGSPTVATLERL
                     GGRERHSHNPLDLGGDASAQLYRDAILALSEDAAVSNVLVILSPHALVDPVEVAQDII
                     DLAAQVRLQLCCCWMGGGQVAEARALLARAGLPVFSSPEAAIELFHNISRFHHNQTLL
                     LQTAGGGSAAAYRRTGGARVLIETLLNQRRHILSAMESKALLRSFGIPVTHTTVARDA
                     TEALFVAEQAGFPVAMKVDAPELASKSESGGVRLNLNTAESVWSAFHDIVDAVRLRHP
                     EVQINGVSIEPYLYRPQARAFRIQVHRDAIFGPVISLGAQDASAPRTFALPPLNPVLA
                     RDLIDSAIPAAAQGPRSQGGALNRAAIEQVLVSVSDLVCELPWVRELAIDPLFIDGDG
                     AIAADARVVIDQGLPAGSDRYAHMAIHPYPLHLVQDWKVGDGRAVRTRPVRPEDANLV
                     LGFFDGLSAETRFMRFMEELQELSPALVARFTQIDYDREMALIATTIDDAGAEHMVGS
                     ARYSLAADGESVEFALVVADDWQRHGLGRRLMGALIEVARGKGYRNIFGDVLGKNAKM
                     LRLMHGLGFLVQPHPEESALRRVVKVLHGK"
     gene            complement(5194..6291)
                     /locus_tag="PKV97_08185"
     CDS             complement(5194..6291)
                     /locus_tag="PKV97_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNB05894.1"
                     /translation="MSAPRFQELAIKRVSPEAAGAVAITFAIPEAERERFAFEPGQFL
                     TLRATIDGQDVRRNYSISSPRSRLAREGELEIGIRPVEGGLFSNWATRAIKAGDTLQV
                     MPPDGRFVVKKKRAIHRVGFAAGSGITPILSIAASTLEEQPDSKFTLIYGNRRMSTVM
                     FNEDLQDLKDRYRDRLTMIHVLSRQAQEVDLLQGRIDGPKVRAIIDALLPVGSMDEVF
                     ICGPDEMITATETALVEAGVPADRIRTERFTANLPAGAHPVGASSTAEAVAAATKDIT
                     MVLVLDGKEHEIAIGPDEHLLDAGLNAGLDLPFSCKAGVCCTCRAKVTEGEVVMDKNF
                     TLEADEVAQGYVLSCQARATTKRLKISFDER"
     gene            complement(6311..6823)
                     /gene="paaJ"
                     /locus_tag="PKV97_08190"
     CDS             complement(6311..6823)
                     /gene="paaJ"
                     /locus_tag="PKV97_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HNB05895.1"
                     /translation="MSSVVDKVWATLEPLTDPEIPVVTLRELGILRDVRETAAGIEVV
                     ITPTYSGCPAMGQIEDDVRASLEQAGIAARVITQLAPAWTTDWMSDEAKEKLRAYGIA
                     PPHRTCGSSGGAEVSVLKFVRRQPTAAVACPQCGSTHTVVTSEFGSTACKALYKCLDC
                     QEPFDYFKPY"
     gene            complement(6835..7617)
                     /gene="paaC"
                     /locus_tag="PKV97_08195"
     CDS             complement(6835..7617)
                     /gene="paaC"
                     /locus_tag="PKV97_08195"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HNB05896.1"
                     /translation="MSTPIDYLLHLADNAVVLGQRNAEWCGHGPILEEDMALANVSLD
                     LIGQARLLYQRAAELKGGDATEDKLAYFRGPHEFRNYTLLELPHHGALVGYAAADRDY
                     ATTIVRNFLYSALMVHVWDGLQQSADASLAAIAAKSLKETSYHLHHSRDWLVRMGDGT
                     EESHARAQAAVNHLMPYTEEFWTTSPAEGAAVAAKIGIDVWALRPAWDALVNEALAEA
                     TLTRPNFHGYVTEGKIGLHSEHLSYLLGEMQGLARAHPGATW"
     gene            complement(7617..>7873)
                     /gene="paaB"
                     /locus_tag="PKV97_08200"
     CDS             complement(7617..>7873)
                     /gene="paaB"
                     /locus_tag="PKV97_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HNB05897.1"
                     /translation="FVRSKQGLEHKHCGSLHAADAAQALHMARDVYTRRQEGVSIWVV
                     PSVAITASNPDDKGELFDPAADKIYRHPTFYEIPEEVGHM"
BASE COUNT         1093 a         2494 c         3018 g         1268 t
ORIGIN      
        1 gcgatgaagg ggttggaggt gtcgaccacc ggcacgcgct ggaagttgat gtgggtgcgg
       61 gcgaactggg ggacgatgta atgcacgtag tcgtgcatgc ggcgcaggat ggtgtcctgc
      121 acggcctcgg cggtgtagcc gcgcagcttg gtgtcgcggt gcagcttctg gatccactcg
      181 aggttgatga tgggcacgac gccgatcagc aggtcgacgt ggctggcgat gtcggcctcg
      241 tcggtgacga gtgcgccgtg caggccctcg tagaacaggc agtcggtgcc gaccgggaga
      301 tcttcccact cggtgaaggt gccgagcggc aggttccagc gcatcgcttc ggcttcgttg
      361 tggatgtagt agcggcggcg gccggtggcg gcctcgccgt aggcgcggaa gaggctctcg
      421 agctcggaga gcaggttggc ttccggcccg aagtggctga tcccgcgcag gcccgcacgc
      481 tcggcttcct cgatcagcgt cttcatctcg gcgcgggtgt ggcggtggaa gctgtcgccc
      541 tcgacgatgg cggcgttgac gttctcgcgg tggaagatcg cttcgaaggt gtgcttgacc
      601 gtagtggtgc cggcgccgga agagccggtg acggcgatga tcgggtgctt catggacatg
      661 atgggttccg ccgctggcga gtcggtttcg acacggattc tgcaccgcaa taaagcggtg
      721 tgcgagccat caggagcgcg ccttgaccca ggccgaccat tcggcgaaca gcgccgggct
      781 ggtggcggcg atgacgccgc gcttgatcgc cgggccggac atcagcacgc cgccgtcgag
      841 cgcctcggcg atgccgccgg cttcggcgag gatcaggcgg ccggcggcgt agtcccacag
      901 catctgcccg ccgtgcaggt agacgtcgag ccggccggcg gcgacgaagc accattcgag
      961 ggcgctggag ccgaaattgc gctgcgagta gtagggcgga cgcaccgcga gctcgtcgcc
     1021 gaggtggtgg ctgatgcgct tgaagtccac cccggcgacg gcgtcgcgca actcggtggc
     1081 gcctgcgcgc agcggcagct cggtgccgtt gaggaaggcg ccggcgccgc gggcggcgta
     1141 gaaggattcg tcggtgatcg ggttgtacac cacgccgatc accggctcgt ggtcgatcag
     1201 ataggcgatc gacaccgcga acagggggat gccgttggcg aagttggtgg tgccgtcgat
     1261 cgggtcgatg caccacaggc cgcgcgcgcc ttccttccac agcgcggcct gctcggccgc
     1321 ggtcatctcc tcgccgagca cggcgccggg caggaaggcg ggcagggcct cgctgaagcg
     1381 ctgctgcgag gccatgtcgg cctcggtgaa cagcgaaccg tcggccttgc ggttgcgcgc
     1441 ggacttcagg tagcgcggaa ggatctcggc gcgcgcgatg tcgcgcacca tcgattcgag
     1501 ggcgcgggca cgggcgaggc ggtgggcggc ggtgggcatg gcgggctcct tgccggcggg
     1561 cggcgggaga ggggcagggg gcgaggggag ctgggcctat aatcttacga tgatttcacg
     1621 attccacttt ccggatcgcc tgcccgcggg cggcgaggtg gcgctccccg aggcgctcgc
     1681 ccaccatgcg ctgcgggtgt tgcgcctgcg cgatggcgag gcggtggtgc tgttcgacgg
     1741 cagcggcgga gaggtcgatg cgcgcctggc cgtgcgcggc aaggcggtgt tcgctcggct
     1801 cggcgaggcg cgtgcggtcg aacgcgagtc gccgttgcgc atcgtgctgg tgcaggcgct
     1861 ggcctcgggc gacaagatgg actgggtggt gcagaaggcg gtggagctcg gcgtgcatgc
     1921 gatccagccg gtgcaggccg agcgctcggt gctgcgtctg gctgccgagc gtgccgacaa
     1981 gcgcgtcgcg cactggcagc aggtcgcggt cgcggcctgc gagcagtgcg ggcgcaaccg
     2041 cgtccccgag attcgccccc tgcagccgct cgcacactgg ctgggcacgc atcgcgctgc
     2101 gcccggctac gtgctcgcgc ccggcggggc caagggcttt gccgacgaac ccgagccgaa
     2161 ggacgaggtg cacctgctgg tcgggccgga gggtggctgg agcgagggcg agatggcggc
     2221 cttcgatgcc gccggttgcc ggcgcgtgcg gctcgggccg cgcgtgctgc gcaccgagac
     2281 cgcgggcctc gccgcggtgg cggcgctgca ggcgcgctgg ggcgattact gagcggccgt
     2341 cccggccgct gcgcacgcgc tacttgccat gcagcacctt caccacccgg cgcaacgcgc
     2401 tctcttccgg gtggggctgc accaggaagc cgaggccgtg catcaggcgc agcatcttgg
     2461 cgttcttgcc cagcacgtcg ccgaagatgt tgcgatagcc cttgccgcgc gcgacctcga
     2521 tgagggcacc catgaggcgg cggcccagtc cgtggcgttg ccagtcgtcg gccacgacca
     2581 gggcgaactc gaccgattcg ccgtcggcgg cgagggaata gcgggcgctg cccaccatgt
     2641 gctcggcgcc ggcgtcgtcg atggtggtgg cgatcagcgc catctcgcgg tcgtagtcga
     2701 tctgcgtgaa gcgcgcgacc agcgccgggc tgagctcctg cagctcttcc atgaagcgca
     2761 tgaagcgggt ctcggcgctc agtccgtcga agaagcccag caccaggttg gcatcctccg
     2821 ggcgcaccgg acgcgtacgt acggcacgac cgtcgccgac cttccagtcc tggacgaggt
     2881 gcagcggata ggggtggatc gccatgtgcg cgtagcggtc gctgccggcg ggaaggccct
     2941 ggtcgatgac gacgcgggca tcggccgcga tggcgccgtc gccgtcgatg aacaaggggt
     3001 cgatcgcgag ctcgcgcacc cagggcagct cgcacacgag gtcggacacc gacaccagca
     3061 cctgctcgat ggcggcgcgg ttgagcgcgc cgccctggct gcgcgggccc tgtgcggccg
     3121 ccgggatggc cgagtcgatg aggtcgcggg ccagcacggg gttcagcggt ggcagcgcga
     3181 aggtgcgcgg cgcggaggcg tcctgcgcgc cgaggctgat caccggaccg aagatcgcgt
     3241 cgcgatgcac ctggatgcgg aaggcgcgcg cctgcggccg gtacagatag ggctcgatcg
     3301 acacgccgtt gatctgcacc tcggggtggc gcaggcgcac cgcgtcgacg atgtcgtgga
     3361 aggcgctcca caccgactcg gcggtgttga ggttgaggcg cacgccgccg gattcggact
     3421 tgctcgccag ctcgggagcg tcgaccttca tcgccaccgg aaaacccgcc tgctcggcga
     3481 cgaacaaggc ctcggtggcg tcgcgggcga cggtggtgtg cgtgaccggg atgccgaagc
     3541 tgcgcaacag cgccttggac tccatcgccg agaggatatg gcgacgctgg ttgagcaggg
     3601 tctcgatcag cacgcgcgca ccacccgtgc ggcggtaggc ggcggcgctg ccgccgccgg
     3661 cggtctgcag cagcagggtc tggttgtggt ggaagcgcga gatgttgtgg aacagctcga
     3721 tcgccgcctc gggcgaactg aacaccggca gcccggcccg ggcgagcagc gcgcgcgcct
     3781 cggcgacctg gccgccgccc atccagcagc agcacagctg gagccgcacc tgggcggcga
     3841 ggtcgatgat gtcctgcgcc acctcgaccg gatcgacgag cgcgtgcggc gacaggatca
     3901 ccagcacgtt ggaaaccgcg gcgtcctcgg agagggcgag gatggcgtcg cggtacagct
     3961 gcgcgctggc gtcgccgccg aggtcgagcg ggttgtggga gtgccgctcg cgtccgccga
     4021 ggcgctcgag cgtggcgacc gtcggcgagc ccagcccgac gagtccggtg ccgaggtcct
     4081 tggcgcggtc ggcggcgaga tcggccgggc cgctgccgtt ggagacgatg gcgaggctgt
     4141 cctcgcgcgg tcgaaagccg caggacaagg ccttggcggc gaagaagagc tggtcgatgt
     4201 tctgcacccg caccacgccg gcgcggcgca tcgccgcgtc gaagaccacg tcggcgaggc
     4261 cggcgttgtc cgcgtcggcg gcgttgttgt cgctgcgacc gtggcggccg gccttgagca
     4321 ggatgatcgg cttgacgcgc gcggccgagc gcagtgcgct catgaactgg cgggcgttgc
     4381 ggatgtgatc gacgtgcagc aggatgtagc gcgtctgcgt gtcgtggatc aggtactcca
     4441 gcgtttcccc gtagtcgatg tccaccgtgc cgccgagcga gatgaccgaa gagaagccga
     4501 cgtcgttggt caacgcccag tcgagcacga ccgagcacat cgcgcccgac tgcgagatca
     4561 gggcgatctc gccgggcttg acggcgatgc gggtgagcgc ggcattgatc ccgctgccgg
     4621 ggcgtgcgat gcccaggctg ccgggcccga tcaggcgcag ccgggtctcg cgcgcggtct
     4681 cgacgatgcg gcgctcgagt gcgttcccgc tggcgctgat gatgaccgcg ttgcgtacgc
     4741 cgctgcgccc gcactgtgcg gtgacgctgt gcagcatgcg tgcgggggtg gcgatgatgg
     4801 cgagatcgac cggtcggccg atgtcctcga ccgagcggtg gcagggatgg tcctggatgc
     4861 tgccgtactt cgggttgacc gcgtacaggg tgccgcgata gccgccttcg accaggttgc
     4921 ggaagatgac gcggccgacg gacccgggtg cgtcggacgc gccgatgacg gcgatcgagg
     4981 acggttcgaa ggcggtgctg agagcgtggg agccggccat gatcttcgcg ctggaaagtg
     5041 gatgaggtct aaagggatag caggccgctg ccctttcggc gttgacctgc gtcaggggcg
     5101 cgcggccgcg tctccgtccg cgaaggaaaa cggccgtcga gacggccgtc ggaagctcct
     5161 gcgcgcgcgg acgccgtcgg cccgcagatc gtgtcagcgc tcgtcgaagc tgatcttcag
     5221 gcgcttggtg gtggcgcgcg cctggcagct cagcacgtag ccctgcgcga cttcgtcggc
     5281 ctcgagggtg aagttcttgt ccatcacgac ttcgccctcc gtcaccttgg cgcggcaggt
     5341 gcagcacacg ccggccttgc acgagaaggg gaggtcgagg ccggcattga ggccggcgtc
     5401 gagcaggtgc tcgtcggggc cgatcgcgat ctcgtgctcc ttgccgtcga gcaccaggac
     5461 catggtgatg tccttggtcg cggccgcgac ggcctcggcg gtgctcgagg cgccgaccgg
     5521 gtgggcgccg gcgggcaggt tggcggtgaa gcgctcggtg cggatacggt cggcgggcac
     5581 gccggcctcg accagcgcgg tctcggtggc ggtgatcatc tcgtcggggc cgcagatgaa
     5641 gacctcgtcc atgctgccca ccggcagcag ggcgtcgatg atcgcgcgca ccttgggacc
     5701 gtcgatgcgc ccctgcagca ggtccacttc ctgggcctgg cgcgacagca cgtggatcat
     5761 ggtcaggcgg tcgcggtagc ggtccttgag gtcctgcagg tcctcgttga acatcaccgt
     5821 ggacatgcga cggttgccgt agatcagcgt gaacttcgag tccggctgtt cttccagcgt
     5881 gctcgcggcg atcgacagga tcggggtgat gcccgagccg gcggcgaagc cgacgcggtg
     5941 gatggcgcgc ttcttcttga ccacgaagcg gccgtcgggc ggcatcacct gcagggtgtc
     6001 gccggccttg atggcgcgcg tggcccagtt cgagaacagg ccgccttcca ccgggcggat
     6061 gccgatctcg agctcgccct cgcgtgccag gcggctgcgc gggctgctga tcgagtagtt
     6121 gcggcgcacg tcctggccat cgatggtggc gcgcagggtg aggaactggc ccggctcgaa
     6181 ggcgaagcgc tcgcgctcgg cctcggggat ggcgaaggtg atcgccaccg cgccggcggc
     6241 ctcggggctc acgcgcttga tggcgagttc ttggaaacgg ggggctgaca tggcgtctgg
     6301 gctccggctc ttaatagggt ttgaaatagt cgaagggctc ctggcagtcg aggcacttgt
     6361 acagcgcctt gcaggccgtg gagccgaact cggaggtgac gacggtgtgg gtggagccgc
     6421 actgcggaca ggcgaccgcc gcggtcggtt gccggcgcac gaacttcagc acgctgacct
     6481 cggcgccgcc gctgctgccg caggtgcggt ggggcggggc gatgccgtag gcgcgcagct
     6541 tttccttggc ctcgtcgctc atccagtcgg tggtccaggc cggcgcgagc tgggtgatca
     6601 cccgcgccgc gatgccggcc tgttccaggc tcgcacgcac gtcgtcctcg atctgcccca
     6661 tggcggggca gccgctgtag gtcggggtga tgaccacctc gatgcccgcc gccgtctccc
     6721 gcacatcccg caggatgccc agctcgcgca gggtcaccac cgggatctcc gggtccgtca
     6781 ggggctccag ggtggcccag accttgtcca cgacgctgct catggccttc gcccttacca
     6841 ggtggcgccc gggtgggcgc gggccagacc ctgcatctcg ccgagcaggt agctcagatg
     6901 ctcggagtgc aggccgatct tgccctcggt cacgtaaccg tggaagttcg ggcgggtcag
     6961 ggtggcttcg gccagcgcct cgttgacgag cgcgtcccag gccgggcgca gcgcccatac
     7021 gtcgatgccg atcttggccg ccaccgcggc gccttcggcc gggctggtgg tccagaactc
     7081 ctcggtgtag ggcatgaggt ggttcaccgc cgcctgcgcg cgggcgtgcg actcctcggt
     7141 gccgtcgccc atgcgcacca gccagtcacg cgagtggtgc aggtggtagc tggtttcctt
     7201 cagcgacttg gcggcgatcg cggcgaggct ggcgtcggcg gactgctgca ggccgtccca
     7261 cacgtgcacc atcagcgccg aatacaggaa gttgcgcacg atcgtggtgg cgtagtcgcg
     7321 gtcggccgcg gcgtagccga ccagcgcgcc gtggtgcggg agctccagca gcgtgtagtt
     7381 gcggaactcg tgcgggccgc ggaaataggc cagcttgtcc tcggtggcgt cgccgccctt
     7441 cagctcggcg gcgcgctggt acagcaggcg cgcctggccg atgaggtcga ggctgacgtt
     7501 ggccagcgcc atgtcttctt ccaggatggg gccgtgcccg caccattcgg cgttgcgctg
     7561 gccgagcacg acggcgttgt cggccaggtg cagcaggtag tcgatcggcg tgctcattac
     7621 atgtgcccca cttcttcagg gatctcgtag aaggtcggat ggcggtagat cttgtcggcg
     7681 gcggggtcga agagctcgcc cttgtcgtcc gggttgctgg cggtgatggc gaccgagggc
     7741 accacccaga tgctcacgcc ttcctggcgg cgggtgtaca cgtcgcgcgc catgtgcagg
     7801 gcctgggcgg cgtcggccgc gtgcaggctg ccgcagtgct tgtgctcgag gccttgcttg
     7861 ctgcggacga aga
//
LOCUS       DAOQNN010000123         7845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4778_length_7845_cov_9.787163, whole genome shotgun sequence.
ACCESSION   DAOQNN010000123 DAOQNN010000000
VERSION     DAOQNN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4778_length_7845_cov_9.787163"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..267)
                     /locus_tag="PKV97_08205"
     CDS             complement(97..267)
                     /locus_tag="PKV97_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05898.1"
                     /translation="MVIDPPPRQTELILALLSERSLCLCHVLRPHVHQADMADCSAPC
                     DCTSAQLMVGAG"
     gene            complement(383..775)
                     /locus_tag="PKV97_08210"
     CDS             complement(383..775)
                     /locus_tag="PKV97_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05899.1"
                     /translation="MQNKLLADKYPIHVLELGKNETDHVRLDDLLAAIEAKAEADPTV
                     RLIATFDHLSHTKLIGGEIRPDILAAKNLIFCFGVKLLDPNMMAVRPRSIGFAELPDR
                     FVISFLEAPMPEANRKMIEWVTALRKAA"
     gene            1082..1369
                     /locus_tag="PKV97_08215"
     CDS             1082..1369
                     /locus_tag="PKV97_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05900.1"
                     /translation="MSEPSGLEVAGHPHRDDFAARGEHASGRGLGLDDHLATLDLLEA
                     PMDLEDRAERRGSRSSPHSAAVMKRSGGSLPLGRFSGESSWKASMRETKGW"
     gene            complement(1478..3130)
                     /gene="pgi"
                     /locus_tag="PKV97_08220"
     CDS             complement(1478..3130)
                     /gene="pgi"
                     /locus_tag="PKV97_08220"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNB05901.1"
                     /translation="MPAPSSLAAWKSLQQRRRTHLPRSLRERFAADPGRAARMQASAC
                     GITLDYSKNLLDDDELVLLLRLADEADLASRIRAMFGGERINTTEARAVLHVALRCPP
                     GAHWPVGDLDVAAEVHAVRARMRTFCLQVRSGDWRGHGGERITDVVNIGIGGSDLGPA
                     MVCEALADHAGDGPRVHFVSNVDGVHIGDVTRRCDPRSTLFVVASKTFTTQETLANAH
                     AARAWLVGALGDEAAVARHFVAVSTNAAAVRDFGIDTANMFGFWDWVGGRFSLWSAIG
                     LPIALALGPDRFDELLAGAHAMDEHFRTAPPAANLPVLMALLAIWNVNFLDAASQLVA
                     PYHQRLHRLPAYLQQLDMESNGKSVRRDGTPVDCRTSPILWGEQGINGQHAYFQLVHQ
                     GTQLVPVDFIGVLDPGREDEALHRIAFANLVAQAEALMRGKTAAEAEAEMRAAGVPEE
                     RIAALLPHRVFSGDRPSNVVLLERLDPFTLGALVAAYEHRTFVQGVIWDVNSFDQWGV
                     ELGKQLAARVLAELDPHAAAPSADAHDASTRGLIERYRARPR"
     gene            3337..3909
                     /gene="pgsA"
                     /locus_tag="PKV97_08225"
     CDS             3337..3909
                     /gene="pgsA"
                     /locus_tag="PKV97_08225"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNB05902.1"
                     /translation="MPLNIPNALTWARIAMIPVFVGLFYLPESWMPIATQNLVATVIF
                     VAAAVTDWFDGYLARALGQTSAFGAFLDPVADKLMVAAALILLVQLGRVDALIAVVII
                     GREITISALREWMARVGQSASVAVAYVGKLKTTAQMVAIPMLLFDASLYGFDLRLAGE
                     VLIYVAAALTLWSMGYYLRRAMPLLRETEE"
     gene            3982..4057
                     /locus_tag="PKV97_08230"
     tRNA            3982..4057
                     /locus_tag="PKV97_08230"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4015..4017,aa:Gly,seq:gcc)
     gene            4130..4203
                     /locus_tag="PKV97_08235"
     tRNA            4130..4203
                     /locus_tag="PKV97_08235"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4162..4164,aa:Cys,seq:gca)
     gene            4276..4351
                     /locus_tag="PKV97_08240"
     tRNA            4276..4351
                     /locus_tag="PKV97_08240"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4309..4311,aa:Gly,seq:gcc)
     gene            4500..5687
                     /locus_tag="PKV97_08245"
     CDS             4500..5687
                     /locus_tag="PKV97_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNB05903.1"
                     /translation="MPFTIDSTLPLPAGAVLPDYGDGGLYGFARGLRHWLHDRKVGWP
                     AAEVAPGERALVVLLVIDGLGERFLDTVGWGSALHAAKHAGLSSVCPSTTASAITTLA
                     TGVAPVEHGLNGWFIHDRRFGGVIAPLPLIRRSGEPLEAFRLLPRLFPVAPMYRHACR
                     PVTLVSPVQIAFSRFSLHHGRGAHIEPYEGLQDYVAAIVDMADALAHSGGLIHAYYPV
                     FDMLSHQHGCRSAEAVACFTRVDAAFVSLQQALAGRDVRLLVTADHGFIDAPPERRID
                     LAPDGEVAAMLAAPLFGERRLAFCRVRAGAQAEFEAWAADELRGKAVAVRGEDFLAAG
                     LLGPGQVHPRLAERLGSHALLMEAGWTIVDHVAGEHEHTMIGVHGGLSADEMRVPLML
                     ART"
     gene            complement(5772..6230)
                     /locus_tag="PKV97_08250"
     CDS             complement(5772..6230)
                     /locus_tag="PKV97_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HNB05904.1"
                     /translation="MIVLDLHCSNAHRFEGWFGSAAAFEAQLADRLVNCPICGATEVR
                     RLPSAPYVQTGQSASARPPAAGEVQAASAVPPSAPAPEGLARLMTQLRSLARVAENVG
                     ERLPEEARRIHYGETEARDIRGSASADEVESLLEEGILVLPVPPAEEDLH"
     gene            complement(6300..6384)
                     /locus_tag="PKV97_08255"
     tRNA            complement(6300..6384)
                     /locus_tag="PKV97_08255"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6348..6350),aa:Leu,seq:taa)
     gene            complement(6394..6654)
                     /gene="minE"
                     /locus_tag="PKV97_08260"
     CDS             complement(6394..6654)
                     /gene="minE"
                     /locus_tag="PKV97_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNB05905.1"
                     /translation="MSFLARLFGEKKKTAEIAKNRLSLLIAHERAGDSPRADFLPDLQ
                     RELIEVISKYVAVNPDDIKVQLEKQDNYEVLEVNIVLPEQNR"
     gene            complement(6656..7468)
                     /gene="minD"
                     /locus_tag="PKV97_08265"
     CDS             complement(6656..7468)
                     /gene="minD"
                     /locus_tag="PKV97_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNB05906.1"
                     /translation="MRVIVVTSGKGGVGKTTTSAAFASGLALRGFKTAVIDFDVGLRN
                     LDLIMGCERRVVYDLVNVVNGEARLNQALIKDKHCDNLFILPASQTRDKDALTEEGVE
                     KVLQELEHMGFEYVVCDSPAGIERGAVMALTFADEAIVTTNPEVSSVRDSDRILGILQ
                     SKSKRAREGGEPVKEHLLITRYSPKRVEDGEMLSYKDVQELLRVPLIGVIPESESVLH
                     ASNQGTPAIHLKGTDVAEAYADVVGRFLGEDLPLRYVNYEKPGLIKRLFGGK"
     gene            complement(7500..>7845)
                     /gene="minC"
                     /locus_tag="PKV97_08270"
     CDS             complement(7500..>7845)
                     /gene="minC"
                     /locus_tag="PKV97_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNB05907.1"
                     /translation="TPTMFVERPLRSGQQIYARGTDLVLLGGVSPGAEVIADGSIHCY
                     GPLRGRAIAGAQGNQAARIVASNFGPELVSIAGVYRTFERGIPEAFAGKPTLVRLNGT
                     DHTLNLEALKLD"
BASE COUNT         1153 a         2532 c         2837 g         1323 t
ORIGIN      
        1 gcgcgatcgc ggaaagcgag cggctcgccg acgagttcgc cgcgcgcgtc gcgggaggcc
       61 gtccgatcga ggcggaagcg ctcggcgaaa cctgactcag cccgcgccga ccatcagctg
      121 agcgctggtg cagtcgcacg gcgcactgca atcggccatg tctgcctgat ggacatgggg
      181 ccgcagcaca tggcacaggc acaggctgcg ctcgctgagg agggccagga tgagctcggt
      241 ctggcgcggg ggcgggtcga tgaccaccgc ctcctgctcg tcgaggtcgg cgagcaggta
      301 gccgatcggg ccggagtcgg ggctgtgcag gggatgaagg tacgtggcgg cgcgcgcccg
      361 ctccagcggg cggattcggt tgtcaggcgg ccttgcgcag ggcggtgacc cactcgatca
      421 tcttgcggtt ggcctcgggc atcggcgcct cgaggaagct gatgacgaag cggtcgggca
      481 actcggcaaa gccgatcgag cgcgggcgga ccgccatcat gttgggatcg agcagcttca
      541 cgccgaaaca gaagatgaga ttcttcgccg ccaggatgtc ggggcgaatc tcgccgccga
      601 tcagcttcgt gtggctcaga tggtcgaagg tcgcgatcag gcgcacggtg gggtccgcct
      661 cggccttggc ctcgatcgcc gcgagcaggt cgtcgaggcg gacgtgatcg gtttcgttct
      721 tgccgagttc gagcacgtgg atcgggtact tgtcggcgag cagcttgttc tgcatggaac
      781 actccctggg gtcggcgggg gggcggatac ggcggtgccg cattccgggg tggattgtcc
      841 gacgggcccg ctgcgctcgc atggatcgtg cgggccgcgc gagcctcgtc ccaagatgac
      901 taacaggaat cgtgccagcg tcgtgcgatg gcggaagcgg cgtcgttgcc ggcgatccgg
      961 cggtcgaggc gggctcgccg cgtgcccact tgcgccagcg tggcagctcg gctggcgcta
     1021 ctggcagcgc tggcaggcta gtgtcgtcct gcgcccgccc gcaccctgct cgcgacggcg
     1081 cttgtccgaa ccttcgggcc ttgaagttgc cgggcatccg caccgcgacg acttcgccgc
     1141 gcgcggcgag cacgccagcg gccgcggcct tggcctcgac gatcaccttg cgacccttga
     1201 cctccttgaa gcgcccatgg atctcgagga ccgggccgag cggcgtgggt cgaggtcgtc
     1261 gccccacagc gcggcggtga tgaagcgcag cggcggctcg ctgcctctcg ggcgcttctc
     1321 gggggagtcg tcctggaagg cgagcatgcg ggagactaag ggatggtgag ggaaggcttg
     1381 catcctgtgg ccgcgtatgg ccctcgccca tcgcgatcga ggacgcgcgt cgctcccgca
     1441 agaaaggccg ccccgcaggc acctcgcgcc gcggcgctca gcgcgggcgg gcgcggtagc
     1501 gctcgatcag gccgcgcgtg ctggcgtcgt gggcgtccgc cgagggggcg gccgcgtgcg
     1561 ggtcgagctc ggccagcacg cgcgcggcga gctgcttgcc gagctccacg ccccattggt
     1621 cgaagctgtt cacatcccag atcacgccct ggacgaaggt gcggtgctcg taggcggcga
     1681 ccagcgcgcc gagcgtgaag gggtcgaggc gctcgagcag cacaacgttg ctcggccggt
     1741 cgccggagaa gacgcggtgc gggagcaggg cagcgatacg ttcctcggga acgccggccg
     1801 cgcgcatctc ggcctcggct tcggcggcgg tcttgccgcg catcagcgct tcggcctgcg
     1861 ccaccaagtt ggcgaaggcg atgcggtgca gggcctcgtc ctcgcggccg ggatcgagca
     1921 cgccgatgaa atctaccggc acgagctgcg tgccctgatg caccagctgg aaatacgcgt
     1981 gctggccgtt gatgccttgc tcgccccaca ggatggggct ggtgcggcaa tccaccggcg
     2041 tgccgtcgcg gcgcaccgac ttgccgttgg attccatgtc gagctgttgc agataggcgg
     2101 gcaggcggtg caggcgctga tgatagggtg cgacgagctg gctggcggcg tcgaggaaat
     2161 tcacgttcca gatggcgagc agcgccatca gcaccggcag gttggcggca ggcggggcgg
     2221 tgcggaaatg ctcgtccatc gcgtgcgcgc cggcgagcag ttcgtcgaag cgatcggggc
     2281 cgagcgcgag cgcgatgggc aggccgatcg cgctccatag cgagaagcgc ccacccaccc
     2341 agtcccagaa gccgaacatg tttgcggtgt cgatgccgaa gtcgcgcacc gctgcggcat
     2401 tggtcgacac ggcgacgaag tggcgcgcca ccgcggcttc gtcgccgagc gcgcccacca
     2461 gccaggcgcg tgcggcgtgc gcgttggcga gcgtctcctg cgtggtgaag gtcttcgagg
     2521 cgaccacgaa caaggtgctg cgcggatcgc agcggcgcgt cacgtcgccg atgtgcacgc
     2581 cgtccacgtt ggagacgaag tgcacgcgcg ggccgtcgcc ggcgtggtcc gcgagcgcct
     2641 cgcacaccat cgccggaccg aggtcggagc cgccgatgcc gatgttcacc acgtcggtga
     2701 tgcgttcgcc gccgtggcca cgccagtcgc cactgcgcac ctgcaggcag aacgtgcgca
     2761 tgcgcgccct caccgcatgc acctcggcag cgacgtccag gtcgccgact ggccagtgtg
     2821 cccccggcgg gcagcgcagg gccacgtgga ggacggcgcg ggcctcggtg gtgttgatgc
     2881 gttcgccgcc gaacatcgcc cggatgcgac tggcgagatc ggcctcgtcg gccaggcgca
     2941 gcaacagcac cagctcgtcg tcgtcgagca ggttcttcga gtaatccagg gtgatgccgc
     3001 aggcgctggc ctgcatgcgt gcggcgcgcc cgggatcagc ggcgaagcgc tcgcgcaggg
     3061 agcgcgggag atgggtgcga cggcgctgct ggagcgattt ccaggcggcc agggagctgg
     3121 gggcaggcat tggaagggac cggaacggcc gatgcgagga agatggatgc ttgtcgagga
     3181 tcttacctag cgcacgcgtg cctgtgtgca ccggaagcgc tgcagggcgg gactttcggc
     3241 acgctgcgcg cacgcacgga atgcggccgt ccctacgcgc gtatgggcgc ttgatatact
     3301 cgccgctccc cgaacctttg cgagtcgccc tccggcatgc cgctcaacat tccgaatgcg
     3361 ctgacctggg cgcggatcgc catgatcccg gtcttcgtcg gcctcttcta tctccccgag
     3421 tcgtggatgc cgatcgcgac ccagaacctc gtcgccacgg tgatcttcgt cgccgccgcg
     3481 gtgaccgact ggttcgacgg ctacctcgcc cgcgcgctcg gccagacctc ggccttcggc
     3541 gccttcctcg acccggttgc cgacaagctg atggtggccg ccgcgctgat cctgctggtg
     3601 cagctcggtc gagtggatgc gctgatcgcg gtcgtcatca tcggtcgcga gatcaccatc
     3661 tcggccctgc gcgagtggat ggcgcgggtc ggccagtcgg ccagcgtcgc cgtggcctat
     3721 gtcggcaagc tcaagaccac cgcgcagatg gtcgcgatcc cgatgctgct gttcgatgcc
     3781 tccctgtacg gtttcgacct gcgcctggcc ggagaagtgc tgatctacgt cgccgccgcg
     3841 ctcacgctat ggtcgatggg gtactatctg cggcgcgcga tgccgctgct gcgcgagacg
     3901 gaagaatgaa gcggatcgcg agaaagcgca gataggggtt gacgagctaa gaattgtgtc
     3961 tataatggcg gtctttcccg ggcgggaata gctcagttgg tagagcgcaa ccttgccaag
     4021 gttgaggtcg cgagttcgag actcgtttcc cgctccaaag aattcgcagg ctgggcagtg
     4081 cccagccaag tggccccggg gcattggggc agctggccga aacggttgtg gcgcgatggc
     4141 agagtggtta tgcagcggcc tgcaaagccg tgtaccccgg ttcgattccg agtcgcgcct
     4201 ccagtagtaa aaaagttgta aagaagtatt gcgatgcggt gcaaaagaag ctagaatttg
     4261 cgccctgtcg tcaaagcggg aatagctcag ttggtagagc gcaaccttgc caaggttgag
     4321 gtcgcgagtt cgagactcgt ttcccgctcc agattctgaa agccggatgt gcgcccagcg
     4381 cccatccggc ttttgcattg tgcccccgcg gtgcatgcct cgccgacggt ttgccgggtg
     4441 cggccttgca gggtaggctc gaagctcttc cggagctatc tgcgatcctc gcgcaccgca
     4501 tgcccttcac catcgactcc accttgcccc tcccggctgg cgctgttctt cccgactatg
     4561 gcgacggcgg gctctacggc ttcgcgcgcg ggctccgcca ctggctgcac gaccgcaagg
     4621 tcgggtggcc tgcggccgag gtcgcgccgg gcgagcgcgc gctggtcgtg ctgctggtca
     4681 tcgacgggct gggcgaacgc tttctcgaca cggtcgggtg gggctccgcg ctgcatgcgg
     4741 ccaagcacgc cggcctgagc tcggtgtgcc cgagcaccac ggcgagtgcg atcaccacgc
     4801 tggcgaccgg cgtcgcgccg gtcgagcacg gcctcaacgg ctggttcatc cacgatcgcc
     4861 gcttcggtgg cgtgatcgcg cccttgccgc tgatccgccg cagcggcgag ccgctggagg
     4921 ccttccgcct gctgccgcgc ctgttcccgg tggcgccgat gtatcgccac gcctgccgac
     4981 cggtcaccct ggtctcgccc gtgcagatcg cattctcgcg cttctcgctg caccatgggc
     5041 gcggggcaca catcgagcct tacgaagggc tgcaggacta cgtggccgcc atcgtcgaca
     5101 tggccgatgc gctcgcgcac agcggcgggc tgatccacgc ctattacccg gtgttcgaca
     5161 tgctgagcca ccagcacggc tgccgctcgg ccgaggcggt cgcgtgcttc acgcgcgtcg
     5221 atgccgcctt cgtgtcgctg cagcaggcgc tggcggggcg cgacgtgcgt ctgctggtga
     5281 cggccgacca cggcttcatc gacgcgccac ccgagcgccg catcgacctc gcgcccgacg
     5341 gcgaggtcgc cgccatgctc gccgcgccgc tgttcggcga gcgccggctg gctttctgcc
     5401 gggtgcgcgc cggtgcgcag gccgaattcg aagcgtgggc tgcggacgag ctgcgtggca
     5461 aggcggtggc ggtgcgcggc gaagactttc tcgccgccgg cctgctcggc ccgggtcagg
     5521 tgcatccgcg gctggctgaa cgcctgggca gccacgcgct actgatggag gccgggtgga
     5581 cgatcgtgga tcacgtggcg ggcgagcacg agcacaccat gatcggcgtg catggtggcc
     5641 tcagcgcgga cgagatgcgc gtgccgctga tgctggcacg tacctgagga cgggcactcc
     5701 acacggctgc gacagggcgg tggacctggc ggtccgtgct cggcacgcat ccccgcctgc
     5761 gccctggctc ttcagtgcag gtcttcctcg gctggcggga ccggcagcac caggatgccc
     5821 tcttcgagca gggactcgac ctcgtcggcg gaggccgagc cgcgaatgtc gcgcgcctcg
     5881 gtttcgccgt agtggatgcg gcgcgcctcc tcgggcaggc gctcgcccac gttctccgca
     5941 acgcgcgcaa ggctgcgcag ctgcgtcatc aggcgcgcga gaccttcggg agcaggagcg
     6001 gagggaggga cggcgctcgc ggcctgcacc tcacccgctg cgggcggcct tgccgaggcg
     6061 gactggccgg tctggacgta gggcgcgctc ggcaggcggc ggacctcggt ggcgccgcag
     6121 atcgggcagt tcaccaggcg gtccgcgagc tgcgcctcga aggctgcggc ggaaccgaac
     6181 cagccttcga agcggtgtgc attgctgcag tgtaggtcga gtacgatcat ggcgaggact
     6241 gtccggtcac tgtgccgggc aggacgccgc agcaaggatg ggacaatggc tgagcgggat
     6301 ggtgcccggg acgggactcg aacccgtaag gcttgcgccg gcggatttta agtccgctat
     6361 ggttaccgat ttcatcaccc gggcggccga ggctcagcgg ttctgctccg gcagcacgat
     6421 attgacctcg agcacctcgt agttgtcctg cttttcgagc tggaccttga tgtcgtcggg
     6481 gttgaccgcg acgtacttcg agatgacctc gatcagctcg cgctgcaggt cgggcaggaa
     6541 gtcggccctg gggctgtcgc ccgcgcgctc gtgggcgatc agcagcgaca ggcggttctt
     6601 ggcgatctcc gcggtcttct tcttttcgcc gaagaggcgg gcgaggaagg acatgtcact
     6661 tgcctccgaa caggcgcttg atcaggccgg gcttttcgta attgacgtag cgcagcggca
     6721 ggtcttcgcc gaggaagcgg ccgacgacgt cggcgtaggc ttcggcgacg tcggtaccct
     6781 tgaggtggat cgcgggcgtg ccctggttcg aggcgtgcag cacggactcc gactcgggga
     6841 tcacgccgat cagcggcacg cgcagcagct cctgcacgtc cttgtacgac agcatctcgc
     6901 cgtcctcgac gcgcttgggc gagtagcggg tgatgagcag gtgctccttg accggctcgc
     6961 cgccctcgcg cgcacgcttg gacttgctct gcaggatacc gaggatgcgg tcggagtcgc
     7021 gcaccgagga gacctcgggg ttggtggtca cgatggcctc gtcggcgaac gtcagcgcca
     7081 tcaccgcgcc gcgctcgatg ccggccgggg agtcgcacac cacgtactcg aagcccatgt
     7141 gctcgagctc ctgcagcacc ttctcgacgc cttcctcggt gagcgcgtcc ttgtcgcgcg
     7201 tctgcgaggc cgggaggatg aacaggttgt cgcagtgctt gtccttgatc agggcctggt
     7261 tcaggcgggc ctcgccattg accacattga ccaggtcgta cacgacgcga cgctcgcagc
     7321 ccatgatgag gtcgaggttg cgcaggccga catcgaagtc gatgacggcg gtcttgaagc
     7381 cgcgcagggc gagtccggag gcgaatgccg cactggtggt ggtcttgccg acgccgccct
     7441 tgcccgaagt tactacgatg actctcacgg tttccccttg tggatgcggt atggctggct
     7501 cagtcgagct tcagcgcttc gaggttgagt gtatggtcgg tgccgttcag ccgcaccaag
     7561 gtgggcttgc cggcaaaggc ttcgggaatg ccgcgctcga aggtgcggta cacgccggcg
     7621 atcgatacga gttcggggcc gaagttgctg gccacgatgc gcgccgcctg gttgccctga
     7681 gcgcccgcga tcgcgcgtcc gcgcaggggg ccgtagcagt ggatgctgcc atcggcgatg
     7741 acttcggcgc cggggctcac gccaccgagc aggacgagat cggtgccgcg cgcatagatc
     7801 tgctggccgg agcgcagcgg gcgttcgacg aacatggtcg gggtg
//
LOCUS       DAOQNN010000124         7835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4787_length_7835_cov_11.462596, whole genome shotgun sequence.
ACCESSION   DAOQNN010000124 DAOQNN010000000
VERSION     DAOQNN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4787_length_7835_cov_11.462596"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PKV97_08275"
     CDS             <1..318
                     /locus_tag="PKV97_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNB05908.1"
                     /translation="SGCMNSCGHHHVGHIGILGVDKNDEEWYQVSIGGTQGNGTTLGK
                     VIGPSFALDEIVGVIEKLLETYVELRHEDERFIDTVQRVGTEPFKARVYADRQPQRKA
                     AHV"
     gene            311..865
                     /locus_tag="PKV97_08280"
     CDS             311..865
                     /locus_tag="PKV97_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HNB05909.1"
                     /translation="MSDKIIKNGRVVAADRLTVSLAEGEVPADIAVPAGPVLVPLAVW
                     QARREELLARARSGEVGVWLAAGEDVAALADDLGLLQIVGLHFPKFTDGRSYSAATLL
                     RTRYGYQGELRAIGQVLRDQFNYMTRCGFDALQPQAERYTDEQLEAAVASIDDFTQPY
                     QRSVKDPLPLFRRVERGTGREVQQ"
     gene            862..1632
                     /locus_tag="PKV97_08285"
     CDS             862..1632
                     /locus_tag="PKV97_08285"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNB05910.1"
                     /translation="MNAAVSLLRPAPTNAATRPQLTDALRATVGAKSAAALALLREAV
                     AEFGAHGELAFANSFGAEDMVLTDLILSNGLPITIFSLDTGRLPAETYSLIGEVERRY
                     GTKLEIYFPDAGAVETYVRSHGINAFYDTVELRKACCGVRKMEPLRRALTGRKAWITG
                     LRAAQSVTRSGLPVRETDTANGLEKLNPLSDWSETEVWAYIRLAEVPYNALHDQFYPS
                     IGCAPCTRAIAVGEDVRAGRWWWENPETKECGLHVKAS"
     gene            1636..1788
                     /locus_tag="PKV97_08290"
     CDS             1636..1788
                     /locus_tag="PKV97_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05911.1"
                     /translation="MNGKHPAQGTHSPAIGADRRSLQQRLLALRAGLRAALRAFRTHP
                     IKHPQR"
     gene            1796..2710
                     /gene="cysD"
                     /locus_tag="PKV97_08295"
     CDS             1796..2710
                     /gene="cysD"
                     /locus_tag="PKV97_08295"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNB05912.1"
                     /translation="MSTLTKQTLSHLDWLEAEAIHILREVAGQCANPVLLFSGGKDSI
                     CLLRLAEKAFRPGRFPFPLMHIDTGHNYKEVTDFRDRRAAELGERLIVRSVEDSMARG
                     TVVLKHAGESRNKHQSVTLLEAIEEFGFDACIGGARRDEEKARAKERIMSFRDEFGQW
                     DPKNQRPELWNLYNARSHKGENIRAFPISNWTELDVWQYIQREQLELPSIYFAHTRPV
                     VRKNGLLQPVTELTPAKDGDVVEDVRVRFRTVGDITCTAPVESDADTVEKILAETATT
                     TITERGATRMDDQTSEASMEQRKKEGYF"
     gene            2791..4071
                     /gene="cysN"
                     /locus_tag="PKV97_08300"
     CDS             2791..4071
                     /gene="cysN"
                     /locus_tag="PKV97_08300"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNB05913.1"
                     /translation="MSALEHLPDIDNGLLRFLTCGSVDDGKSTLIGRLLFDTKTILAD
                     TLNAIEKTSAKRGMSAVDLSLLTDGLQAEREQGITIDVAYRYFSTGRRKYIIADAPGH
                     EQYTRNMVTAASTANLAIILIDARKGVLTQTRRHSYLASLVGIPHLVVAVNKMDLVDY
                     DQAVFERIKGEYLEFAAKLGIRDVRFIPISALEGDMIVDRGERLSWYDGPTLLDILEN
                     APAAHTEQAEDFRFPVQFVCRPQESANPELHDYRGFMGRVESGEVAVGDAVTVLPSGA
                     SSRVRKIELGGVALPRAIHEQSVTLLLEDEIDISRGDLIVKSAEAREPVKQIEATVCW
                     LSETPLSPARTYLVRHTTREVKAKLAKIDYRLDVNTLEKEATTGLAMNDIARVTLKLA
                     QPIIADRYTENRATGAFIIIDESTNNTVGAGMIG"
     gene            4310..5047
                     /locus_tag="PKV97_08305"
     CDS             4310..5047
                     /locus_tag="PKV97_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HNB05914.1"
                     /translation="MLKRITLVTVATLVLAGCAATHVAISKRDLDVQTKMSDTVFLDP
                     VGPDKRTIFLQIRNTSDKPDLAVNDEIAAALQTKGYTLLQDPEAAHYVLQANILQVGK
                     SAPTAIEAAIDQGYGVAASGGLVLGAGAAYAGGASGRGIAAAGLIGSLAEGIAGAAVK
                     DVYFSMITDLQIKERIRGAGKARVSSNHSLRQGNSGGSQVSYDEESSYKTYQTRIGST
                     ANKVNLAFEEALPELKKGLINSISGLF"
     gene            5146..5547
                     /locus_tag="PKV97_08310"
     CDS             5146..5547
                     /locus_tag="PKV97_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNB05915.1"
                     /translation="MTDRNPSDTARTRLDKWLWAARFFKHRTGATEAVDGGKVKLNGM
                     AAKPARELKVGDRLDITIGEDTRTVIVRAIADKRGSAAVAQALYEETAESIEARERAR
                     ELRKFAAAPGADLHGRPTKRDRRRMDRFGGC"
     gene            complement(5610..6485)
                     /locus_tag="PKV97_08315"
     CDS             complement(5610..6485)
                     /locus_tag="PKV97_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNB05916.1"
                     /translation="MLIRIVSILFPLFAITALGYFVGRRMKPDLSHANKLNMDVFVPA
                     LVFGALVGKDFRLGEYLPLLFATVVIIVGSGVAGWLVARAAGIATKTLVPPMMFNNCG
                     NLGLPLAVLAFGESALAPAVVMFVVSNVIHFSYGAWLLDHHTKLSTVWRSPSILATIA
                     GLAVGFLGIEVWPPLLMSIEMVGDISIPLMLFALGVRLADSRISAVGFGLFGAVLRPV
                     VGMALAWALLQMLELPPREQALLLVFGALPPAVLNYMFAERYDQEPDKVASMVLIGNL
                     AAVVFLPIALALVLD"
     gene            complement(6604..>7835)
                     /gene="hslU"
                     /locus_tag="PKV97_08320"
     CDS             complement(6604..>7835)
                     /gene="hslU"
                     /locus_tag="PKV97_08320"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNB05917.1"
                     /translation="RSQVAEPLKSEITPKNILMIGPTGVGKTEIARRLARLANAPFIK
                     IEATKFTEVGYVGRDVDTIIRDLMEIAVKDGRERAMKSVRDRALDAAEDRVLDALLPP
                     ARPVGFNAEPEPAQDSSTRQKFRKKLREGELDDKEIEIEVAAPSMQAEIFAPPGMEEL
                     TQQIQGMFQNLGGGKKKQRKLQIREAMKLLADEEAARLINDEEVKLEAVRAVEQNGIV
                     FLDEVDKIAARSDVQGADVSRQGVQRDLLPLVEGTTISTKYGMIKTDHILFIASGAFH
                     LSKPSDLIPELQGRFPIRVELESLSVEDFARILTSTDACLTRQYEALLATDGVKLEFA
                     DDGIRRLAEIAYQVNEKTENIGARRLYTVMEKLLEEVSFEAGRSSAEQTVVVDAAYVD
                     SRLVMLAQREDLARYVL"
BASE COUNT         1424 a         2779 c         2493 g         1139 t
ORIGIN      
        1 tccggctgca tgaactcctg cggccaccac cacgtcggcc acatcggcat cctcggcgtc
       61 gataagaacg acgaggagtg gtaccaggtc agcatcggcg gcacacaggg caacggcacc
      121 acgctcggca aggtgatcgg tccgtccttc gccctcgacg agatcgtggg cgtgatcgag
      181 aagctgctcg aaacctacgt cgaactgcgc catgaggacg agcgcttcat cgacaccgta
      241 caacgcgtcg gcaccgagcc cttcaaggca cgcgtgtatg ccgaccgcca gccgcaaagg
      301 aaagccgccc atgtctgaca agatcatcaa gaacggccgc gtcgtcgccg ccgaccgcct
      361 gaccgtgagc ctcgccgaag gcgaggtgcc tgccgacatc gcggtgcccg ccggccccgt
      421 gctggtgccg ctcgccgtat ggcaggcgcg ccgcgaagag ctgctggcgc gtgcgcgcag
      481 cggcgaggtg ggcgtgtggc tggccgcagg cgaggacgtc gccgcactcg ctgacgacct
      541 cgggctgctg cagatcgtcg gcctgcactt ccccaagttc accgacggcc gcagctactc
      601 cgcggcaacc ttgctgcgca cccgctacgg ctaccagggc gaactgcgcg cgatcggcca
      661 ggtcctgcgc gaccagttca actacatgac gcgctgcggc ttcgatgccc tgcagccgca
      721 agccgagcgc tacaccgacg aacagctcga ggcggcggtg gccagcatcg acgacttcac
      781 ccagccctac cagcgctcgg tgaaggaccc gctgccgctg ttccgccgcg tcgagcgcgg
      841 caccggccgc gaggtgcagc aatgaacgct gccgtcagcc tgctgcgccc cgccccgacc
      901 aacgccgcca cccgcccgca gctcaccgac gcactgcgcg ccaccgtggg tgccaagtcc
      961 gccgccgcgc tcgcactgct gcgtgaggcc gtcgccgagt tcggcgcgca cggcgagctc
     1021 gccttcgcca acagcttcgg cgccgaggac atggtgctga ccgacctcat cctgtcgaac
     1081 ggcctgccga tcaccatctt ctcgctcgac accgggcggc tccccgccga gacctacagc
     1141 ctgatcggcg aagtcgagcg ccgctacggc acgaagctcg agatctactt ccccgacgcg
     1201 ggcgcggtcg aaacctatgt gcgcagccac ggcatcaacg ccttctacga cacggtcgag
     1261 ctgcgcaagg cctgctgcgg cgtgcgcaag atggagccgc tgcgtcgcgc gctcaccggc
     1321 cgcaaggcct ggatcaccgg cctgcgcgca gcccagtcgg tcacccgctc cggcctgccg
     1381 gtacgcgaga ccgacaccgc caacgggctg gagaagctca acccgctgtc ggactggagc
     1441 gagaccgagg tgtgggccta catccgcctc gccgaggtgc cctacaacgc cctgcacgac
     1501 cagttctacc ccagcatcgg ctgcgccccc tgcacccgcg cgatcgcggt cggcgaggac
     1561 gtgcgcgccg gacgctggtg gtgggaaaac ccggagacca aggagtgcgg tctccacgtc
     1621 aaggcgagct gagccatgaa cggcaagcac cccgcccagg gcacgcacag ccccgcgatc
     1681 ggtgccgacc gccgcagcct gcagcagcgc ctgctcgcgc tgcgcgccgg actcagggcg
     1741 gcgctgcgcg ccttcagaac ccatccgatc aagcatccgc aacggtaacc gcaccatgtc
     1801 cacgctgacc aaacagacgc tgtcccacct cgactggctc gaggccgaag ccatccacat
     1861 cctgcgcgaa gtcgccggcc agtgcgccaa cccggtgttg ctgttctccg gcggcaagga
     1921 ttcgatctgc ctgctgcgcc tggccgagaa ggcctttcgc ccgggccgct tccccttccc
     1981 gctgatgcac atcgacaccg gccacaacta caaggaagtc accgacttcc gcgaccggcg
     2041 cgccgccgaa ctgggcgagc gcctgatcgt gcgctcggtg gaagactcga tggcgcgcgg
     2101 caccgtggtg ctgaagcacg cgggcgagtc gcgcaacaag caccagtcgg tgacgctgct
     2161 cgaggcgatc gaggagttcg gcttcgacgc ctgcatcggc ggcgcccgcc gcgacgagga
     2221 gaaggcgcgt gccaaggaac gcatcatgag cttccgcgac gagttcgggc agtgggaccc
     2281 gaagaaccag cgtcccgagc tgtggaacct gtacaacgcg cgtagccaca agggcgagaa
     2341 catccgcgcc ttcccgatca gcaactggac cgaactcgac gtgtggcagt acatccagcg
     2401 cgagcagctg gagctgccat cgatctactt cgcccacacc cgcccggtgg tgcgcaagaa
     2461 cggcctgctg cagccggtga ccgagctgac gccggcgaag gatggcgacg tcgtggagga
     2521 cgtgcgggtg cgcttccgca ccgtggggga catcacctgc acggcgccgg tggaatccga
     2581 cgcggacacc gtggagaaga tcctcgccga gacggcgacc accacgatca ccgagcgcgg
     2641 tgcgacgcgg atggacgacc agacctcgga ggcgtcgatg gagcaacgca agaaggaagg
     2701 ctacttctga gcgcatccgc gctcgtcgca gccccccccc cccccccact cttcgcacct
     2761 cacgcctgac ctttgcacgg aatcatgacc atgtccgccc tcgagcacct ccccgacatc
     2821 gacaacggcc tgctgcgctt tctcacctgc ggcagcgtcg acgacggcaa gagcaccctg
     2881 atcggccgcc tgctgttcga caccaagacc atcctcgccg acaccctcaa cgccatcgag
     2941 aagacctcgg ccaagcgcgg catgagcgcg gtcgacctgt cgctgctgac cgatggcctg
     3001 caggccgagc gcgagcaggg catcaccatc gacgtcgcct atcgctactt ctccaccggc
     3061 cggcgcaagt acatcatcgc cgacgccccc ggccacgagc agtacacccg caacatggtg
     3121 accgccgcgt ccaccgccaa cctggcgatc atcctcatcg acgcgcgcaa gggcgtgctg
     3181 acccagaccc gccgccactc ctacctcgcc agcctggtcg gcattcccca tctggtggtc
     3241 gcggtcaaca agatggacct ggtcgactac gaccaggcgg tgttcgagcg catcaagggg
     3301 gaatacctcg aattcgccgc caagctcggc atccgggacg tgcgcttcat cccgatctcg
     3361 gcgctggagg gcgacatgat cgtcgaccgc ggcgagcgcc tgtcctggta cgacggcccg
     3421 accctgctcg acatcctcga gaacgccccc gccgcccaca ccgaacaggc cgaggacttc
     3481 cgcttcccgg tgcagttcgt gtgccgaccg caagagtccg ccaaccccga actgcacgac
     3541 tatcgcggct tcatgggccg ggtcgaatcg ggcgaggtcg cggtgggcga cgcggtcacc
     3601 gtactgccct cgggcgccag cagccgggtc aggaagatcg agctcggcgg cgtcgcgctg
     3661 ccgcgcgcca tccacgagca gtcggtgacc ctgctgctcg aagacgagat cgacatctcg
     3721 cgcggcgacc tgatcgtgaa atccgccgag gcgcgggagc cggtgaagca gatcgaagcc
     3781 acggtgtgct ggctgtccga gaccccgctg tcgcctgcac gcacctacct ggtacgccac
     3841 accacgcgcg aggtaaaggc caagctcgcg aagatcgact accgcctgga cgtgaatacc
     3901 ctggaaaagg aggccaccac cgggctggcg atgaacgaca tcgcccgcgt cacgctcaag
     3961 ctcgcccagc cgatcattgc cgaccgctac acggagaacc gcgctaccgg cgccttcatc
     4021 atcatcgacg agagcaccaa caacaccgta ggcgcgggca tgatcggctg atcgccgccc
     4081 gccctccgca gcgagcgaac cccggccccc gcaagggcgc cggggttctt cattttgggg
     4141 acgaagaaat tcttaccgca tcacctaaca tgacaatcgg gcatgaagct ctctataatg
     4201 aaattccata cgattatttt gagatctttc agtacctgca aaagtcatcc ccggatcgca
     4261 ccgaaaaccg ctgcctgcca tcttcatccg tcctcgagga agtctcccga tgctcaagcg
     4321 aatcaccctc gtcaccgtcg ccaccttggt cctcgccggc tgcgccgcca cccacgtcgc
     4381 catcagcaag cgcgacctcg atgtgcagac caagatgtcc gacacggtct tcctcgaccc
     4441 ggtcggccct gacaagcgca ccatcttcct gcagatccgc aatacctccg acaagcccga
     4501 ccttgcggtg aatgacgaga tcgccgccgc gctgcagacc aagggttaca ccctgctgca
     4561 ggacccggag gcggcgcact acgtcctgca ggcgaacatc ctgcaggtcg gaaaatcggc
     4621 acccaccgcg atcgaggcgg cgatcgacca aggctacggg gtagccgcct cgggcggcct
     4681 ggtcctgggg gcgggagcgg catacgccgg cggcgccagc ggccgtggca tcgccgccgc
     4741 cggcctgatc ggctcgctgg ccgaaggcat cgcgggcgcc gcggtcaagg acgtgtattt
     4801 ttcgatgatc accgacctgc agatcaagga gcgcatccgc ggtgccggca aggcaagggt
     4861 gagttcgaac cacagcctgc gccagggcaa cagcggtggt tcgcaggtgt cgtacgacga
     4921 agagtcgtcc tacaagactt atcagacccg catcggctcg accgcgaaca aggtcaacct
     4981 cgccttcgag gaggcgctgc ccgagttgaa gaaaggcctg atcaattcga tctccgggct
     5041 gttctgaacg ctcgcgggct tcatctgcgc cagcacgccg ccgggtgttc gataatcacg
     5101 gcggcgcctt gccaatccgg cgcccttcga ccgagcgcca ccatcatgac cgaccgcaat
     5161 ccctccgaca ccgcgcgtac ccgcctcgac aagtggctgt gggcggcacg tttcttcaag
     5221 caccgtacgg gcgcaaccga ggcggtcgat ggaggaaagg tcaagctcaa cggaatggcg
     5281 gccaagccgg cacgcgaact caaggtcggt gaccgcctcg acatcacgat cggcgaggat
     5341 acgcgcacgg tgatcgtgcg cgcgatcgcg gacaagcgcg gctcggccgc cgtggcgcag
     5401 gcgctctacg aggagaccgc ggaaagcatc gaggcgcgcg aacgcgcccg tgaattgcgc
     5461 aagttcgccg ccgcgcccgg cgccgacctg cacggccggc cgaccaagcg cgaccgccgc
     5521 aggatggacc gcttcggggg atgctgagct cgcgtcgcca agcgcgagcc ctcgcggtgc
     5581 ccgatccgta ccggctccgc tcccgtgcct cagtcgagta ccagcgccag cgcgatcggc
     5641 aggaaaacca ccgcagcgag gttgccgatc agcaccatcg aggccacctt gtcgggctcc
     5701 tggtcgtaac gctcggcgaa catgtagttc agcaccgccg gcggcagggc gccgaacacg
     5761 agcaacagcg cctgctcgcg cggcggaagc tcgagcatct gcagcagggc ccaggccagc
     5821 gccatgccca ccaccgggcg tagcaccgca ccgaagaggc cgaagccgac ggcgctgatg
     5881 cgcgaatcgg ccaggcgcac gccgagcgcg aacagcatca gggggatcga gatgtcgccg
     5941 accatctcga tcgacatcag cagcggcggc cacacctcga tgccgaggaa gccgaccgcc
     6001 aggcccgcga tcgtcgccag gatggagggc gagcgccaca ccgtgctcag cttggtatgg
     6061 tggtccagca gccaggcacc gtaggagaag tggatcacgt tcgacaccac gaacatcacc
     6121 accgccggcg ccagcgccga ctcgccgaag gcgagcaccg ccagcggcag gccgaggttg
     6181 ccgcagttgt tgaacatcat cggcggcacc agcgtcttgg tggcgatccc ggccgcacgc
     6241 gcgaccagcc agccggccac accggagccg acgatgatca ccactgtcgc gaacagcagc
     6301 ggcaggtatt cgccgaggcg gaagtccttg ccgaccagcg caccgaacac cagcgcgggc
     6361 acgaagacat ccatgttcag cttgttggcg tgcgacaggt ccggcttcat gcgccggccg
     6421 acgaaatagc ccagcgcggt gatcgcgaac agcgggaaca ggatggagac gattcggatc
     6481 agcattcagg gcaaaccggg agcggactga agggggtgcg acggatgcgg tcacggacaa
     6541 gcccgtcgtc gcgcggtccc cgcggacgaa gcgagcttgt ccgcgaacgg acgcgagccg
     6601 ggatcaaagc acgtaacgcg ccagatcctc gcgctgggcg agcatgacga gccggctgtc
     6661 gacataggcg gcgtcgacta ccacggtctg ctccgcactg ctgcgcccgg cctcgaagga
     6721 aacctcttcg agcagcttct ccatgacggt gtacagccgg cgcgcgccga tgttctcggt
     6781 cttctcgttc acctggtagg cgatctcggc caggcggcgg atgccgtcgt cggcgaattc
     6841 cagcttcacc ccgtcggtgg cgagcagcgc ctcgtactgg cgcgtgaggc aggcgtcggt
     6901 gctggtgagg atgcgggcga agtcctccac agacagcgac tccagctcga cgcggatcgg
     6961 gaaacgcccc tgcagctcgg ggatcagatc cgagggcttg gacaggtgga aggcgccgct
     7021 ggcgatgaac aggatgtgat cggtcttgat catgccgtac ttggtggaga tcgtcgtgcc
     7081 ctcgaccagc ggcagcaggt cgcgctgcac gccctgacgg gagacatctg cgccctgcac
     7141 gtcgctgcgc gcggcgatct tgtccacctc gtcgaggaag acgatgccgt tctgctcgac
     7201 cgcgcgcacg gcctcgagct tgacctcctc gtcgttgatc aggcgcgcgg cctcctcgtc
     7261 ggcgagcagc ttcatggcct cgcggatctg cagcttgcgc tgcttcttct tgccgccgcc
     7321 gaggttctgg aacatgccct ggatctgctg ggtgagttcc tccatgcccg gcggggcgaa
     7381 gatctcggcc tgcatcgagg gcgcggcgac ctcgatctcg atctccttgt cgtcgagttc
     7441 accctcgcgc agcttcttgc ggaatttctg ccgggtggac gaatcctgcg ccggctcggg
     7501 ctcggcgttg aagccgaccg gacgcgccgg cggcagcagg gcgtcgagca cgcggtcctc
     7561 ggcggcatcc agcgcgcggt cgcgcaccga cttcatcgca cgctcgcggc cgtccttgac
     7621 cgcgatctcc atgaggtcgc ggatgatggt gtcgacgtcg cggccgacat agccgacctc
     7681 ggtgaacttg gtcgcctcga tcttgatgaa gggcgcgttg gccaggcgcg ccaggcggcg
     7741 cgcgatctcg gtcttgccga cgccggtggg gccgatcatg aggatgttct tgggggtgat
     7801 ctcgctcttc aggggctcgg ccacctggct gcgcc
//
LOCUS       DAOQNN010000125         7745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4876_length_7745_cov_11.538231, whole genome shotgun sequence.
ACCESSION   DAOQNN010000125 DAOQNN010000000
VERSION     DAOQNN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4876_length_7745_cov_11.538231"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /gene="scpA"
                     /locus_tag="PKV97_08325"
     CDS             <1..1588
                     /gene="scpA"
                     /locus_tag="PKV97_08325"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNB05918.1"
                     /translation="QNDILKEFMVRNTYIYPPTPSMKIIADIFGYTAQHMPKFNSISI
                     SGYHIQEAGANQAIELAFTLADGVEYVRTGINSGMDVDTFAGRLSFFWAVGMNFYLEI
                     AKMRAARLLWWRLMKQFNPKSAKSMMLRTHSQTSGWSLTEQDPYNNVVRTTIEAMAAV
                     FGGTQSLHTNALDEAIALPTEFSARIARNTQIIIQEETHICNVVDPWAGSYMMEKLTQ
                     DMADKAWALIEEIESMGGMTRAVESGWAKMKVEECAADKQARIDSGKDVIVGVNKYKL
                     AKEDPIDILDIDNHAVREAQIARLDKIRATRDTAAVQAALAALTKCAETGEGNLLDLS
                     VKAIRLRATVGEISDALEKVFGRYRANPQAVSGVYGAVVEEQEDWKTLKADIEAFIAE
                     EGRRPRVMIAKLGQDGHDRGAKVVASAFADLGFDIDIGPLFQTPEEAARHAVENDVHA
                     VGCSSLAAGHKTLVPAVIDELKRLGADDIITFVGGVIPAQDYDTLYAAGAKGIFGPGT
                     PIPAAAREVLKQIRAARKAT"
     gene            1797..2840
                     /gene="meaB"
                     /locus_tag="PKV97_08330"
     CDS             1797..2840
                     /gene="meaB"
                     /locus_tag="PKV97_08330"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNB05919.1"
                     /translation="MPAPISTPLDPADQSLVAGVLARQLRPLAKAITLIESQREDHQA
                     RAQKVLEALLPHTGGAMRVGISGVPGVGKSTFIEALGLYLIEQGLRVAVLAVDPSSSV
                     TGGSILGDKTRMELLSQNPAAFIRPSPSACSLGGVAEKTRETMLVCEASGFDVIIVET
                     VGVGQSETAVAGMTDIFCLLQLPNAGDDLQAIKKGIMEIADLIVINKADIDAGAAMRA
                     KAQIKTALHMLRPASPNWTVPVLTLSALQKQGIADFWKAVCDYREALGASGEFAAKRR
                     HQALAWMWEMIDSGLRSRFRSHPRVREELPALARAVEEGDTTPAAAAFRLLGHLQSNE
                     QGRLPRQPAETEI"
     gene            2856..4388
                     /locus_tag="PKV97_08335"
     CDS             2856..4388
                     /locus_tag="PKV97_08335"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNB05920.1"
                     /translation="MQDIIRQLEKKRELARLGGGQKRIDAQHRKGKLTARERIELLLD
                     DDSFEEWDMYKEHRCTEFGMDAEHIPGDGVVTGYGTVNGRLVFVFSQDFTVFGGSLSE
                     THAEKICKVMDHAMKVGAPVIGLNDSGGARIQEGVDSLGGYADVFQRNVLASGVIPQI
                     SLIMGPCAGGAVYSPAMTDFIFMVKDSSYMFVTGPEVVKTVTHEEVTAEELGGAVTHN
                     TKSGVADLAFENDVEALMMTRRLIGFLPSSNREKPPAIPCADSADRIDLSLDTLVPEN
                     PNQPYDMKELIFKMVDDGDFFELQPDYAKNIIIGLARMEGSPVGIVANQPLVLAGCLD
                     IKSSIKAARFVRFCDAFNIPVVTLVDVPGFMPGTAQEYGGIIKHGAKLLYAYAECTVP
                     KVTVITRKAYGGAYDVMSSKHLRGDVNLAWPSAEIAVMGAKGAVEIIFREEKGDPEKL
                     AQREAEYKARFANPFVAASRGFIDDVVMPHNTRKRICRSLAMLRDKQLDNPWRKHGNI
                     PL"
     gene            4412..6400
                     /gene="accC"
                     /locus_tag="PKV97_08340"
     CDS             4412..6400
                     /gene="accC"
                     /locus_tag="PKV97_08340"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNB05921.1"
                     /translation="MFKKILIANRGEITCRVIKTARKMGIKTVAVYSEADKNALHVEL
                     ADEAVCIGPAPSKESYLVMDKIIAACKQTGAEAVHPGYGFLSENAEFSRRLEEEGIKF
                     IGPKHYSVAKMGDKIESKKLAIAAGVNTIPGYNDAISGPDEAVEIAKKIGYPVMIKAS
                     AGGGGKGLRVAFNDKEAHEGFASCVNEARNAFGDDRVFIEKYVLEPRHIEIQVLGDSH
                     GNYVYLNERDCSIQRRHQKVIEEAPSPFVDPAMRKAMGEQAVALARAVNYESAGTVEF
                     VVSGATKEFYFLEMNTRLQVEHPVTELITGLDLVEQMIRVAYGEKLPLAQADVKIDGW
                     AMECRINAEDPFRGFLPSTGRLIKFQPPAEGNGVRVDTGVYEGGEISMFYDSMIAKLI
                     VHGKDRQDAIERMRDALNGFVIRGISSNIPFQAALLQHPRFCSGHFNTGFIAEEYPKG
                     FDASMVPHDDPALLAAVATFARMRYIERAVQIEGQLAGHGRKVASEWVVVMGGKQYAM
                     RTAAIEGGLAVTCEGRTYNIVSDWKFGELLFAGTVNGAPIGLQIERRGLRYRISHFGL
                     QVEPVVMTVRAAHLLSLMPAKLPPDLSKFLLSPMPGLLREVAVTEGQDVKAGEKLAII
                     EAMKMENVLKAEQDGKVKKIVAKPGASLSVDEVIIEFE"
     gene            complement(6653..7507)
                     /locus_tag="PKV97_08345"
     CDS             complement(6653..7507)
                     /locus_tag="PKV97_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNB05922.1"
                     /translation="MDFRNGRESDNVEDRRGQGGRGLRGGGKIGIGTIVLALVAMYFG
                     IDPSVVLDTAMTDPAPVESTQTRPAAENAQARFVSMVLADTEDTWRTIFQQGGATYRE
                     PKLVLYTGATRSACGVGQAEMGPFYCPADAKVYLDLAFFDELHHRFGAPGDFAQAYVI
                     AHEIGHHVQNLLGISDKVQQARTRVSEREANQLSVRLELQADCLAGIWAHHANRSRSV
                     LEAGDIEEGLAAATAVGDDHIQKRSQGYAVPDSFTHGSAEQRRRWFAIGLQQGQFKSC
                     DTFSAPRL"
BASE COUNT         1397 a         2765 c         2429 g         1154 t
ORIGIN      
        1 ccagaacgac atcctgaaag agttcatggt gcggaacacc tacatctatc cgcccacccc
       61 gtcgatgaag atcatcgccg acatcttcgg ctacaccgcg cagcacatgc cgaagttcaa
      121 cagcatctcg atttccggtt accacatcca ggaggcgggc gccaaccagg ccatcgagct
      181 cgccttcacg ctcgccgacg gtgtcgagta cgtgcgcacc ggcatcaact ccggcatgga
      241 cgtcgacacc ttcgccggcc gcctgtcctt cttctgggcg gtgggcatga acttctacct
      301 cgagatcgcc aagatgcgcg ccgcgcgcct gctgtggtgg cggctcatga agcagttcaa
      361 ccccaagagc gccaagtcga tgatgctgcg cacgcactcg cagacctccg gctggtcgct
      421 caccgagcag gacccctaca acaacgtcgt gcgcaccacg atcgaggcca tggctgcggt
      481 gttcggcggc acccagtcgc tgcacaccaa cgcgctcgac gaggcgatcg ccctgcctac
      541 cgagttctcc gcccgcatcg cgcgcaacac ccagatcatc atccaggaag agacccacat
      601 ctgtaacgtg gtcgacccct gggccggctc ctacatgatg gagaagctca cccaggacat
      661 ggccgacaag gcctgggcgc tgatcgagga gatcgagtcc atgggcggca tgacccgcgc
      721 ggtcgaatcc ggctgggcca agatgaaggt cgaggagtgc gccgccgaca agcaggcgcg
      781 catcgactcg ggcaaggacg tgatcgtcgg cgtgaacaag tacaagctcg ccaaggaaga
      841 cccgatcgac atcctcgaca tcgacaacca tgcggtgcgc gaggcgcaga tcgcccgcct
      901 cgacaagatc cgcgccaccc gcgacaccgc cgcggtgcag gccgcgctcg ctgcgctcac
      961 caagtgcgcc gagacgggcg aaggcaacct gctcgacctg tcggtcaagg ccattcgcct
     1021 gcgtgccacc gtcggcgaga tctccgacgc gctcgagaag gtcttcggcc gttaccgtgc
     1081 caatccgcaa gccgtttccg gcgtctatgg agccgtcgtg gaagaacagg aagactggaa
     1141 gaccctcaag gccgacatcg aggccttcat cgccgaagaa ggccgccgcc cgcgcgtgat
     1201 gatcgccaag ctcggccagg acggccacga ccgcggcgcc aaggtggtgg cctcggcctt
     1261 cgccgacctc ggcttcgaca tcgacatcgg cccgctcttc cagaccccgg aagaagccgc
     1321 ccgccacgcg gtcgagaacg acgtccatgc ggtcggctgc tccagcctcg ccgccggcca
     1381 caagaccctg gtcccggccg tcatcgacga gttgaagcgc ctcggcgccg acgacatcat
     1441 cactttcgtc ggtggcgtga tcccggcgca ggactacgac acgctgtacg ccgccggcgc
     1501 caaggggatc ttcggccccg gcacgccgat cccggcggcc gcacgcgagg tgctgaagca
     1561 gatccgcgcc gcacgcaagg cgacgtaagg tcgatgtagg agcgaggcaa gtcgcgatcg
     1621 cagcgcccgg gcaggcgccg ccgacctcac gcttcccgta ggagcgacgc aagtcgcgat
     1681 tgcagcggat cagcgggcgg cgtgcgcatc gtcgaggcgg gaatcgcgac ttgcgtcgct
     1741 cctacaagaa ccacgaaccg caaaccgtag aacggacgcc ccctataaga cccgacatgc
     1801 ctgctccgat atccacccct ctcgatcccg ccgaccagtc gctggtcgct ggcgtgctgg
     1861 cgcgccagct gcggccgctg gccaaggcca tcacgctgat cgaatcgcag cgcgaggacc
     1921 atcaggcgcg cgcgcagaag gtgctcgagg cgctgctgcc gcataccggc ggggcgatgc
     1981 gggtggggat ctcgggcgtg ccgggcgtgg gcaagagcac cttcatcgag gcgctcgggc
     2041 tctacctcat cgagcagggc ctgcgcgtcg cggtgctcgc ggtcgatccg tcctcgtcgg
     2101 tcaccggtgg ctccatcctc ggcgacaaga cgcgcatgga gctgctgtca cagaaccccg
     2161 cggccttcat ccgccccagc ccctcggcct gcagcctcgg cggcgtggcc gagaagacgc
     2221 gcgagaccat gctggtgtgc gaagcttcgg gcttcgacgt catcatcgtc gagaccgtcg
     2281 gcgtcgggca gtccgagacc gcggtcgccg gcatgaccga catcttctgc ctgctgcagc
     2341 tgcccaacgc cggtgacgac ctgcaggcga tcaagaaagg gatcatggag atcgccgacc
     2401 tgatcgtcat caacaaggcc gacatcgacg ccggtgcggc catgcgcgcc aaggcgcaga
     2461 tcaagaccgc gctgcacatg ctgcgcccgg ccagccccaa ctggaccgtg ccggtgctca
     2521 ccctgtcggc gctccagaag cagggcatcg ccgacttctg gaaggcggtg tgcgactacc
     2581 gcgaggcgct cggcgcctcc ggggagttcg ccgccaagcg tcgccaccag gcattggcct
     2641 ggatgtggga gatgatcgat tccgggctgc gcagccgttt ccgcagccat ccccgcgtgc
     2701 gcgaggaact tcccgccctc gcacgcgcgg tcgaggaggg cgacaccacg cccgcggccg
     2761 cggccttccg gctgctcggc cacctccaat ccaacgaaca gggccgactg ccgcgacagc
     2821 cggccgaaac cgagatctga caggagatcg cactcatgca agacatcatt cgccagctcg
     2881 agaaaaagcg cgaactggcc cgcctcggtg gcggccagaa gcgcatcgac gcgcagcatc
     2941 gcaagggcaa gctcaccgcg cgcgagcgca tcgaactgct gctcgacgac gacagcttcg
     3001 aagagtggga catgtacaag gagcaccgct gcaccgagtt cggcatggat gccgagcaca
     3061 tccccggtga cggcgtggtc accggctacg gcaccgtcaa cggccgcctg gtcttcgtct
     3121 tctcgcagga cttcaccgtc ttcggcggct cgctctccga gacccacgcc gagaagatct
     3181 gcaaggtcat ggaccacgcc atgaaggtcg gcgccccggt catcggcctc aacgactcgg
     3241 gcggagcgcg catccaggaa ggcgtggact cgctcggcgg ctacgccgac gtgttccagc
     3301 gcaacgtgct ggcctccggg gtcatcccgc agatctcgct gatcatgggc ccctgcgccg
     3361 gcggcgcggt gtacagcccg gcgatgacgg acttcatctt catggtcaag gactcgtcct
     3421 acatgttcgt gaccggtccc gaggtcgtga agaccgtgac ccacgaggaa gtcaccgccg
     3481 aggagctcgg cggcgccgtc acccacaaca ccaagtccgg cgtcgccgac ctcgccttcg
     3541 agaacgacgt cgaggcgctg atgatgacgc gtcgcctgat cggcttcctg ccctccagca
     3601 accgcgagaa gccgcccgcg atcccctgcg ccgactccgc cgaccgcatc gacctgtcgc
     3661 tcgacaccct ggtcccggag aacccgaatc agccctacga catgaaggag ctgatcttca
     3721 agatggtcga cgacggcgat ttcttcgagc tgcagcccga ctacgccaag aacatcatca
     3781 tcggcctggc gcgcatggaa ggctcgccgg tgggcatcgt cgccaaccag ccgctcgtgc
     3841 tggccggctg cctcgacatc aagagctcga tcaaggccgc ccgcttcgtg cgcttctgcg
     3901 acgccttcaa tatcccggtc gtcaccctgg tcgatgtccc cggcttcatg cccggcaccg
     3961 cgcaggaata cggcggcatc atcaagcacg gcgccaagct gctctacgcc tacgccgagt
     4021 gcaccgtgcc caaggtcacc gtgatcaccc gcaaggccta cggcggcgcc tacgacgtga
     4081 tgtcgtccaa gcacctgcgc ggcgacgtga acctcgcctg gccgagcgcc gagatcgcgg
     4141 tgatgggcgc caagggcgcg gtcgagatca tcttccgcga agagaagggc gaccccgaga
     4201 agctcgccca gcgcgaagcc gaatacaagg cccgtttcgc caaccccttc gtcgccgcca
     4261 gccgcggctt catcgacgac gtggtcatgc cgcacaacac ccgcaagcgc atctgccgct
     4321 cgctggccat gctgcgcgac aagcagctcg acaacccgtg gcgcaagcac ggcaacatcc
     4381 cgctctgacc cgcgcacgag gaaccgacca aatgttcaag aaaatcctga ttgcaaaccg
     4441 cggtgaaatc acctgccgcg tcatcaagac cgcccgcaag atgggcatca agaccgtcgc
     4501 ggtctactcc gaggcggaca agaacgcgct ccacgtcgag ctcgccgacg aagccgtctg
     4561 catcggcccg gcgccctcga aagagtccta cctggtcatg gacaagatca tcgccgcctg
     4621 caagcagacc ggcgccgagg cggtccaccc gggctacggc ttcctgtccg agaacgccga
     4681 gttctcgcgc cgcctcgaag aggaaggcat caagttcatc ggcccgaagc actactcggt
     4741 cgccaagatg ggcgacaaga tcgagtccaa gaagctcgcc atcgcggccg gcgtgaacac
     4801 catccccggc tacaacgacg ccatctccgg ccccgacgag gcggtcgaga tcgcgaagaa
     4861 gatcggctac ccggtcatga tcaaggcctc cgccggcggc ggcggcaagg gcctgcgcgt
     4921 cgccttcaac gacaaggagg cgcacgaggg tttcgcctcc tgcgtcaacg aggcgcgcaa
     4981 cgccttcggc gacgaccgcg tcttcatcga gaagtacgtg ctcgagccgc gccacatcga
     5041 gatccaggtg ctgggcgact cgcatggcaa ctacgtctac ctgaacgagc gcgactgctc
     5101 catccagcgc cgtcaccaga aggtcatcga ggaagccccc agccccttcg tcgaccccgc
     5161 gatgcgcaag gccatgggcg agcaggccgt ggccctggcg cgcgcggtga actacgagtc
     5221 ggccggcacg gtcgagttcg tggtcagcgg cgcgaccaag gagttctact tcctcgagat
     5281 gaacacccgc ctgcaggtcg agcacccggt caccgagctc atcaccgggc tggacctcgt
     5341 cgagcagatg atccgcgtcg cctacggcga aaagctgccg ctcgcccagg ccgacgtcaa
     5401 gatcgacggc tgggcgatgg aatgccgcat caacgccgaa gaccccttcc gcggcttcct
     5461 gccctccacc ggccgcctca tcaagttcca gcccccggcc gaaggcaacg gcgtgcgcgt
     5521 cgacaccggc gtgtatgaag gcggcgagat ctcgatgttc tacgactcga tgatcgccaa
     5581 gctcatcgtg cacggcaagg accgccagga tgcgatcgag cgcatgcgcg acgccctcaa
     5641 cggcttcgtc atccgcggca tcagctccaa catccccttc caggccgcgc tgctgcagca
     5701 tccgcgcttc tgctcgggcc acttcaacac cggcttcatc gccgaggagt atccgaaggg
     5761 cttcgacgcc tccatggtcc cgcacgacga ccccgcgctg ctcgccgccg tcgccacctt
     5821 cgcccgcatg cgctacatcg agcgcgccgt gcagatcgaa ggtcagctcg ccggccacgg
     5881 ccgcaaggtc gccagcgaat gggtcgtcgt catgggcggc aagcagtacg ccatgcgcac
     5941 cgccgccatc gaaggcggcc tcgccgtcac ctgcgaaggc cggacctaca acatcgtctc
     6001 cgactggaag ttcggcgagc tgctcttcgc gggcaccgtc aacggcgccc cgatcggcct
     6061 gcagatcgaa cgccgcggcc tgcgctaccg catctcgcac ttcggcctgc aggtcgaacc
     6121 cgtcgtgatg accgtgcgcg cggcccacct gctgtcgctg atgcccgcga agctgccgcc
     6181 tgacctctcc aagttcctgc tctcgcccat gcccggcctg ctgcgcgagg tcgcggtgac
     6241 cgagggccag gacgtcaagg ccggcgagaa gctcgccatc atcgaagcca tgaagatgga
     6301 aaacgtcctc aaggccgagc aggacggcaa ggtcaagaag atcgtcgcca agcccggcgc
     6361 cagcctgtcg gtggacgagg tcatcatcga gttcgagtga tcgccacccg aaaaccgttt
     6421 tgatcgatgg ggagggagac ccgggccacg cccgggtgcc tgtcggcacg aggcctgcgc
     6481 aagcaggcct cttttttgtc cggccaccgc gggaccggaa cgcgcgccgc ggacgggctc
     6541 ccgggtgacg tcggtgcgat gtgttgcgtc gctcctacgc gaaaacaatc ggcgtcatcg
     6601 ccctggacgg gctcccgggc gatggcgccg tgcctttgcg gcgacggcgt aactacaacc
     6661 gcggcgcgct gaacgtgtcg caggacttga actgcccctg ctgcaatccg atcgcgaacc
     6721 accgccggcg ctgctcggca ctgccgtggg tgaagctgtc cggcaccgcg taaccctggc
     6781 tgcgcttctg gatgtggtcg tcgcccaccg cggtggcggc ggcgaggcct tcctcgatgt
     6841 cgccggcctc gagcacgctg cggctgcggt tggcgtggtg ggcccagatg cccgcgaggc
     6901 aatccgcctg cagttccagg cgcaccgaga gctggttcgc ctcgcgctcc gacacccgcg
     6961 tgcgcgcctg ctgcaccttg tccgagatgc ccagcaggtt ctgcacgtgg tggccgatct
     7021 cgtgcgcgat cacgtacgcc tgggcaaagt cgccgggcgc gccgaagcgg tggtgaagct
     7081 cgtcgaagaa ggccaggtcg agatacacct tggcgtccgc cggacagtag aacggcccca
     7141 tctccgcctg cccgaccccg caggcgctgc gcgtcgcgcc ggtgtagagc acgagcttgg
     7201 gctcgcggta ggtcgcgccc ccctgctgga agatcgtccg ccaggtgtcc tcggtgtcgg
     7261 ccagcaccat cgacacgaag cgcgcctgcg cgttctcggc ggcggggcgg gtctgggtgg
     7321 attcgaccgg cgcggggtcg gtcatggccg tgtcgaggac cacgctcgga tcgatcccga
     7381 agtacatcgc caccagcgcg agcacgatcg tcccgatgcc gatcttgccc ccgccgcgca
     7441 ggccgcgccc gccttgtccg cggcggtcct cgacgttgtc gctctccctg ccgttgcgga
     7501 agtccatggt gcgctccatt cgtggggaac ctcttcagct tagtgagtgc cgagggggca
     7561 aggcaagcgc aagccatccg cgcagcggct ctccggcagg cgcgacgcaa gtcgcgaatg
     7621 cggcgcttcc gcccgcgccg cgctgcccga aggccggaat cgcgacttgc gtcgctccta
     7681 cgtgaaccaa atcaaggcca tcgccttgga tcgtcgcccg ggcagcgcgc cacgcatcgc
     7741 ccgat
//
LOCUS       DAOQNN010000126         7706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4910_length_7706_cov_9.728924, whole genome shotgun sequence.
ACCESSION   DAOQNN010000126 DAOQNN010000000
VERSION     DAOQNN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4910_length_7706_cov_9.728924"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PKV97_08350"
     CDS             complement(<1..320)
                     /locus_tag="PKV97_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05923.1"
                     /translation="MSMMTSTMGSLPRRCHVVMPGFSEGANVGIAVPGRLGLEMTALN
                     FGPEPLAQTVVRAVNDVQGIDPATELAMLIGAMYGWDDARADPAFLRRHHDHRFFAEG
                     VTDEA"
     gene            complement(317..2770)
                     /locus_tag="PKV97_08355"
     CDS             complement(317..2770)
                     /locus_tag="PKV97_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HNB05924.1"
                     /translation="MFSEGLRLKGFRGIRDGMGLDEIVLDFERLVPGAELVALAGPNG
                     KGKTTIMDNLHPYPVMPSRAGKDGLGKFSYYDHLCSAEAEKDLIWRHDGRRYRSHLVF
                     RMNGKKKTEAFLLEWRIDRWVPVTIEDGTVSDGKMETYWRCIIAILGTPETFFTSVFS
                     AQGRQPLSSYTSASIKTLFAELLGHAEILKMSGAAGEVAKLLKTGLPSVRSALTTMET
                     EADSARQAKQELAGATAGEAASRATREAAQKALLEARDRLTTANAQAAGDEGNARRRE
                     AIRTRMIEADKAWEDAVDRLKQQKRGEEARRSAIDRRVRERVGQAAASRRELTQRMER
                     LQGVVVHERVIRSAARLLPLVQALLARRIELEAQCQTRVQAARETRSTVEALRKEIEG
                     IEREAGRATLTVEDLKRRHGLSDEVPCKGTDLQGHCNLLGDAMTAKTLLPSAQHAIQV
                     LQAERANAATRLAEAERDLAALAGAAAAYDAAERKRASLAGRLQRRDKLASRLEDLEQ
                     AKAELQQVQVQLQGLGPADDQPTAEEREEYRLVDEALRNIATALRAEDAKRAAATADL
                     SRQLGELPAPFDKGLIERAAENLRQAQTDFDRADRRLTQAVQRVQTLSDSASRLEGLE
                     ARVKQERARVAYIEAKVAVWTLLAKALGNDGIIALSIDDAGPTLSALANDLLLGCYGP
                     RFTVSLLTQVESAKGEAREGFSIVVHDAESGQSKALEHTSGGERVWINECLTRAIALY
                     LAQNSGRRYQTLFSDEADGALDEERKRMYMRMKRTVLRLGGYEREFFISQTPELTAMA
                     DAIIDLGELQREQQAKEAV"
     gene            complement(2760..3077)
                     /locus_tag="PKV97_08360"
     CDS             complement(2760..3077)
                     /locus_tag="PKV97_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05925.1"
                     /translation="MNSLPEFCYVFHKDQSPGKRIVMVVRGQTGYWPVRDLPDATDEE
                     ALRSIRLANESLGIDGVQAFCMKMGSMFGFHASGADPTWVREHMPEVDENDRSGEGVR
                     NVQ"
     gene            complement(3131..4417)
                     /locus_tag="PKV97_08365"
     CDS             complement(3131..4417)
                     /locus_tag="PKV97_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase family protein"
                     /protein_id="HNB05926.1"
                     /translation="MSDLHYSGKNLAESEACFAHTVALAIAGHAECVVVSGDSTDHAL
                     DAHSPAFVALARNIRRLADHCPVLMLQGTFSHEPPGTLSVLRLLGGRYPVFVADRIQQ
                     VALMPDGTWNESEGWRFENQPAGARLLLSCIPTVNKASVAAVVGATQAAEAVGEQMAN
                     LLAGYAPAHRAARAAGIATAVVSHGTVSGCTTEHGVPMAGLDHEFTTGSLFAADASAV
                     MLGHIHRHQAWQQDGRMIAYAGSIGRFHYGEEGDKGMLVWDVGADGADAVLELTPARR
                     TIELRFAGAPDMEELRAIASDAQGAFVKVCWEISEEDRASIDRDAIEAALVGAAEVKM
                     EGRIIPVVRSRADGISRAATLDEKLVRWAEVAGVVCAPLRDALGDLTIETPETIAAAI
                     LSTTTFASDVDVPIEMTELHGQTLPHEETETLDLFA"
     gene            complement(4460..>5149)
                     /locus_tag="PKV97_08370"
     CDS             complement(4460..>5149)
                     /locus_tag="PKV97_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05927.1"
                     /translation="DIARMRGGRIAGFLKDREAFWLTKRLKDVSHIDENGRAVRVPHW
                     IFGLEVMIDPTRLLAAPEEEVVAAGDLAASVLQGTAKRIIEPESETVHCEDPAFGSFE
                     SGTHQFAHSVDDGGRAAAPFAIRQDGGSERGSSPRPQHVAATAAPASKPPAHAGGAGG
                     STSPQEVVAAAQGAGLDADMFVRYAAKKWGQGWSKNPNGVRKALDEIASYRDNPDALA
                     DRMKAEVEIFF"
     assembly_gap    5150..5249
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5436..7568)
                     /gene="dinG"
                     /locus_tag="PKV97_08375"
     CDS             complement(5436..7568)
                     /gene="dinG"
                     /locus_tag="PKV97_08375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008729.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HNB05928.1"
                     /translation="MITDEEQALIRQCMSALQAGLPGFQTRRQQLQMIAAVAHALGSC
                     RTEDDGGTTGHNLALCQCGTGTGKSIAGTVPALVMAKSRGKRLVISSSTIALQQQYSD
                     KDIPTLQRLLPLDFTFAVAKGRRRYACTAKLFAALDDQRQGDLALHDAAQGAATSPSD
                     ATSLRQLIQLAQSFDCGAWNGDRDELKQPVADEVWERVTTDRQGCAGNRCPHFANCPF
                     YSARQRLKEVDLIIANHDLVLASLQMNGRGVLPDAADTFYIFDEGHSLVAKVVEQFAG
                     RHALKGGVEWLRGLGDCVRDIVLGLQLDHELYRGARAICRELSADLEQLHRALDRTGA
                     FDEKPIRRFKNGVIPDWTRNPGTNILARSTDLQQTVALLREQMFGRASTEATLVTRLV
                     AELGFFASKLENLVDTWTLMLADDSLQGVPNARWIERTGAKGSHDYLVCASPISGADK
                     LRRLLWNRAGAAVVTSATLASCDSFAPYLNQAGLSIYPNVRTLRVNSPFDYRNKARLI
                     LPPMKSDPKEARAHTQEIAEILPRLLTTRGSLMLFASGRQMREVHALLPERLRQRTLM
                     QGQMPKKEMIARHKAAIDEGGTSILFGLGSFAEGVDLPGEYCTHVVIAKLPFSVPDSP
                     LEEARAEWITSLGRSPFVEIALPEASVRLQQAVGRLLRTVDDHGTVTILDRRITTKRW
                     GSALLKGLPDFTVLAGKSANSAIAEIAA"
BASE COUNT         1259 a         2557 c         2375 g         1415 t
ORIGIN      
        1 gcctcgtctg tcacgccttc ggcaaagaac cgatggtcgt ggtggcgcct gagaaacgcg
       61 gggtcggcgc gtgcgtcgtc ccatccgtac atcgcaccaa tgagcatcgc gagctcggtg
      121 gccgggtcga tgccctgcac gtcgttgact gcccgaacta ccgtctgcgc caatggttct
      181 ggcccgaagt tcagagccgt catctcgagg ccaaggcgcc cggggactgc aatgccgacg
      241 ttggcacctt ccgaaaaacc tggcatgacg acatggcacc gtctcggcaa gctacccatc
      301 gtcgacgtca tcatgctcat accgcttcct tggcctgttg ctcgcgttgc agctcaccca
      361 agtcgatgat cgcatcggcc atcgccgtca gctccggggt ctgcgagatg aagaactccc
      421 gctcgtaacc gcccaggcgc aataccgtcc gcttcattcg catgtacatc cgcttgcgct
      481 cctcgtcgag cgcgccgtcc gcttcgtcgg aaaacagcgt ctggtaccgc cgcccactgt
      541 tctgcgctag gtacaaagca atggcgcgcg tcaggcattc gttgatccag acgcgctcgc
      601 cgccgctggt gtgctccagt gccttcgact gtcccgactc ggcgtcatgg accacgatcg
      661 agaagccctc gcgcgcctcg cccttagccg attcgacttg agtcagaagc gagaccgtga
      721 atcgagggcc gtagcagccg agcagcaggt cgttggccag cgccgaaagc gtcgggccgg
      781 cgtcgtcgat cgacaacgcg atgatgccgt cgttgccgag cgccttggcg agcagggtcc
      841 agacggcgac cttcgcttcg atgtaggcaa cgcgagcgcg ctcctgcttc actctcgctt
      901 cgaggccttc gagtctcgag gcggagtcgg acaaggtctg gacccgctgt actgcctgcg
      961 tgagtcgacg atctgcgcga tcgaaatcag tctgcgcctg gcgcaggttt tcggctgcac
     1021 gttcgatcag acccttgtcg aacggcgccg gcagctcgcc gagctgccga ctcagatcgg
     1081 ccgtcgccgc ggcgcgtttc gcatcctcgg ccctcaatgc agttgcgatg ttccgcagtg
     1141 cctcgtcgac cagacggtac tcttcgcgct cctcggcagt cggctgatca tccgctggac
     1201 ccagtccctg aagctgcact tgaacctgct gaagctcggc tttggcctgt tcgagatcct
     1261 caagccgcga agcgagcttg tcgcgccgct gcaaacgtcc ggcgagcgag gcgcgcttgc
     1321 gctccgcagc gtcgtaggcc gccgccgccc cggcgagcgc cgccagatcg cgttcagcct
     1381 cggcgagcct cgtcgccgcg ttggcgcgct ccgcctgcag cacctggatg gcatgctggg
     1441 ccgatggcag tagagtcttg gccgtcatgg catcgcccaa caaattgcag tgcccctgga
     1501 ggtcggtgcc cttgcacggc acttcgtcac tcagaccgtg ccgccgtttc agatcctcga
     1561 ccgtcagggt tgcccggccg gcttccctct cgatgccttc gatttccttg cgcagcgcct
     1621 ccaccgtgct cctggtttcg cgcgccgcct gaacgcgcgt ctgacactgc gcttccaact
     1681 cgatgcggcg ggcaaggagc gcctgcacca acggcaggag ccgcgccgcg ctacgaatga
     1741 cgcgctcgtg gacgacgaca ccctgcaagc gttccatgcg ctgagtcagt tcgcgccggc
     1801 tcgccgccgc ttgtccgacc cgctcccgca cgcgacggtc aatcgccgac ctgcgcgcct
     1861 cctctcccct cttttgctgc ttgagccgat cgacggcgtc ctcccacgcc ttgtcggctt
     1921 cgatcatcct ggtgcggatc gcttcgcgcc ggcgcgcatt gccttcgtcg cccgccgcct
     1981 gcgcattcgc cgtcgtcagc cggtcgcgcg cctcgagcag cgccttctga gcggcctcgc
     2041 gcgtcgctct cgacgccgct tctccggccg tggcgccggc gagctcctgt ttcgcctggc
     2101 gcgccgagtc cgcctcggtc tccatggtcg tcagcgcgct gcgcaccgac ggaaggccag
     2161 tcttgagcag ctttgcgact tcgccggccg cgcccgacat tttcaggatc tcggcatggc
     2221 ccaggagttc ggcgaagagc gtcttgatcg acgcgctggt ataggacgac agaggctgcc
     2281 gcccctgcgc cgaaaacacc gaggtaaaga aggtctccgg cgtgccaagg atcgcgatga
     2341 tgcagcgcca gtaggtttcc atcttgccgt cggatacggt gccatcctcg atggtcacgg
     2401 gaacccagcg gtcgatccgc cattcgagca ggaaggcctc cgtcttcttc ttgccattca
     2461 tgcggaatac caggtggctg cgatagcgcc gaccgtcgtg acgccagatc agatccttct
     2521 ccgcctcggc agagcacaag tgatcgtagt aggagaactt gccgagcccg tccttccccg
     2581 cgcgcgacgg catcacagga taggggtgca gattgtccat gatggtggtc ttgcccttgc
     2641 cgttggggcc ggcaagggcc accagctcgg cgccgggcac caggcgttcg aagtcgagga
     2701 cgatctcgtc gagccccatg ccgtcccgga tgccgcggaa gcccttgagc cgcaggcctt
     2761 cactgaacat tgcgtacccc ctcccccgat ctgtcgttct catcgacctc cggcatgtgc
     2821 tcgcgcaccc acgtcggatc ggcgccgctc gcatggaatc cgaacatcga ccccatcttc
     2881 atgcaaaacg cttgaacgcc gtcgatgccc agcgattcat tggccagccg gatcgagcga
     2941 agcgcctcct cgtcagttgc atccggcagg tcgcgaaccg gccaatatcc ggtctggccg
     3001 cgtacgacca ttacgattct cttccccgga gattggtcct tgtggaagac atagcagaat
     3061 tcaggcaagc tattcatcgt cactccctct tcagaacgaa gacatcagta tcgttgcgcc
     3121 gatcgatcga tcaagcgaac aagtccaggg tttcggtctc ttcgtgaggc agggtctggc
     3181 cgtgcagctc ggtcatctcg atcggaacgt cgacgtcgga tgcgaaagtc gtggtgctca
     3241 atatcgcggc cgcgattgtt tcgggcgtct cgatggtcag gtcgccaagg gcatcacgta
     3301 gcggcgcgca aacgaccccg gcaacctccg cccagcgtac gagcttttcg tcgagcgtcg
     3361 cagctcgcga tatgccgtcg gcgcgtgagc gcaccacggg aatgatgcgg ccttccatct
     3421 tcacctcggc ggcgccgacg agcgcggcct cgatcgcgtc gcgatcgatg ctggcacgat
     3481 cctcctcgct gatctcccag cacactttca cgaaggcgcc ctgtgcatcg ctcgcgatgg
     3541 cgcgcaactc ttccatgtcg ggcgccccgg caaagcgcag ctcgatcgtc ctacgcgccg
     3601 gcgtgagctc gagtacggca tcggcaccgt ctgcgccgac gtcccagacc agcatgccct
     3661 tgtcgccttc ctcgccgtag tggaagcgtc cgatggagcc ggcatacgcg atcatccttc
     3721 cgtcctgctg ccacgcctga tgccggtgaa tgtggcccag catcacggcc gaggcatccg
     3781 ccgcgaacag cgagccggtg gtgaactcgt gatcgaggcc agccatcggc acgccgtgct
     3841 cggtcgtgca tccgctcacg gtgccatggg agacaaccgc agtcgcgatg cccgcggcgc
     3901 gcgcggcgcg gtgcgcaggc gcatagccgg caagcagatt ggccatctgc tcgccaacgg
     3961 cttcggcagc ttgcgtagcc ccgacgaccg cggcgaccga cgccttgttg accgtaggga
     4021 tgcaggacag cagcaagcgc gcgcccgcgg gctggttctc gaaccgccac ccttccgatt
     4081 cgttccaggt gccatccggc atcagggcga cctgctggat ccggtctgcg acgaacaccg
     4141 gatacctgcc gcccaacagc cggagcacgg acagcgtgcc aggcggctcg tgcgagaacg
     4201 tgccttggag catcagcacc gggcaatgat ccgcgagccg ccgaatgttg cgggccagtg
     4261 cgacgaacgc cggtgagtga gcgtcgaggg catggtcggt agagtcgccg gagaccacca
     4321 cgcactccgc gtgtcccgcg atcgcgagcg cgacggtatg cgcgaagcag gcctccgact
     4381 cggccaggtt cttgcccgaa tagtgcaggt cgctcatgtg gcagacccga acagctgatt
     4441 gaatcggcat gtacagctcc tagaagaaga tctcgacttc ggccttcatc ctgtcggcga
     4501 gagcatcggg gttgtcgcgg tagctcgcga tctcgtcgag cgccttgcgc accccgttgg
     4561 gattcttcga ccacccttga ccccacttct tggcggcgta gcggacgaac atgtcggcgt
     4621 cgaggcctgc gccctgtgcg gccgcaacaa cctcttgcgg gctcgtgctc ccgccggcgc
     4681 cgcccgcgtg cgctggaggc ttcgacgccg gagcggccgt ggcggcaacg tgttggggtc
     4741 gcgggctcga ccccctctcg ctgccgccgt cctgccggat tgcgaaaggc gccgcggcgc
     4801 ggccaccgtc gtcgacggaa tgagcgaact ggtgcgtgcc cgactcgaac gacccaaacg
     4861 cgggatcctc gcaatggaca gtctcggact ccggttcgat gatgcgtttg gccgttccct
     4921 gcagtacgga cgcggccaga tctccagcgg cgacaacttc ttcctcgggc gccgcgagca
     4981 gtcgcgttgg atcgatcatc acctcgaggc cgaagatcca atgcgggaca cgcaccgcgc
     5041 gcccgttctc gtcgatgtgt gagacgtcct tgagcctctt cgtcagccag aaggcttcgc
     5101 gatccttcag aaaacccgca atccggccgc cgcgcatgcg ggcgatgtcn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtcatcatcc cgtccgaatg cggcgcttca
     5281 agtgcgggaa atcggatgcc aactggacgc ccagcgccgc gcggttcaca tgcatgcgcc
     5341 gtcaggtgtg gtgctgagac gtgaagagag ctgcagagaa tcgtttccgc tcggacagcg
     5401 aggaaggcag gacaccggtg gaaccgcgac cacggtcaag ccgcgatctc ggcgatcgcg
     5461 ctgttggcgc tcttgccggc gagcacggtg aagtcgggca agcccttgag cagtgccgat
     5521 ccccatcgtt tggtcgtgat gcgtctgtca agaatcgtca cggttccgtg gtcgtcgacc
     5581 gtgcgcagca accgcccgac ggcctgctgc agacgcacgc tggcctcggg caaggcgatc
     5641 tccacgaatg gcgatctacc gaggctggtg atccactcgg cgcgcgcctc ctcgagcgga
     5701 gagtctggaa ccgagaacgg caacttcgca atgacgacat gagtgcagta ctcgcctggc
     5761 aggtcgaccc cctcggcaaa cgagcccaac ccgaagagga tcgaggtgcc gccttcgtcg
     5821 atcgccgcct tgtgtcgcgc gatcatttcc ttcttgggca tctggccctg catcagcgtg
     5881 cgctggcgca accgctcggg tagtaaggca tggacctcgc gcatctggcg cccactcgcg
     5941 aaaagcatga gactgccgcg ggtggtcagc agcctcggca ggatctcggc gatctcctgc
     6001 gtatgcgccc gggcttcctt cggatcggac ttcatcggcg gcaggatgag gcgcgccttg
     6061 ttccggtaat cgaatggcga gttcacccga agcgtcctca cgttgggata gatggagagg
     6121 ccggcctgat tcaagtacgg cgcgaacgag tcgcacgatg cgagagtcgc cgacgtcacg
     6181 accgcagctc cagcccggtt ccagagcagg cggcgcagtt tgtccgcgcc actgatcggc
     6241 gaagcgcaga cgaggtagtc atgactgccc tttgctccgg tccgctcgat ccatcgtgca
     6301 ttgggcacgc cctgcaggct gtcgtctgcc agcatcagcg tccaggtgtc gaccaggttc
     6361 tcgagcttgg acgcgaagaa gcccaattcc gcaaccaggc gcgtgaccag tgtggcttct
     6421 gtgcttgcgc gcccgaacat ctgttcgcgc agcagcgcca ccgtttgctg caggtccgta
     6481 gagcgggcga ggatgttggt gcccggattg cgggtccaat cgggtatgac cccgttcttg
     6541 aagcgcctga tgggcttctc gtcgaacgcg ccggtgcgat cgagcgcgcg gtgcaactgc
     6601 tcgaggtccg ccgaaagctc cctgcaaatc gcgcgcgctc ctcggtagag ctcgtggtcc
     6661 agctgcagcc cgagcacgat gtcgcgcacg cagtcgccaa ggcctcggag ccactccacg
     6721 ccacccttca aggcatggcg gccggcaaat tgctccacca ccttggcgac cagtgaatgg
     6781 ccctcatcga agatgtagaa ggtgtccgca gcatcgggca ggacgccgcg gccgttcatc
     6841 tgcagcgatg cgagcaccag gtcgtgattg gcgatgatta ggtcgacttc ctttaggcgc
     6901 tgccgggccg aatagaacgg gcagttcgcg aagtggggac agcgattacc ggcgcaacct
     6961 tggcgatcgg tcgtcacccg ttcccatact tcgtcggcca ccggctgctt cagctcgtcg
     7021 cgatcgccgt tccatgcacc gcagtcgaag ctctgcgcca gttgaatcag ctggcgcagg
     7081 gatgtggcgt cgctcgggct ggtggccgcc ccttgagccg catcatggag cgccagatcg
     7141 ccttgacgct ggtcgtcaag tgctgcgaag agcttcgcgg tgcaggcgta gcggcgtcgg
     7201 cccttggcca ccgcgaaagt gaagtcgagc ggcagcaacc gctggagagt tggaatgtcc
     7261 ttgtcgctgt attgctgctg gagggcaatc gtcgagctcg agatcaccag gcgcttaccg
     7321 cgcgatttgg ccatcacaag ggccggcact gtgcctgcga tgctcttgcc ggtcccggtg
     7381 ccgcactggc acagcgctaa attgtgcccc gtcgtgccgc cgtcatcctc tgttcggcag
     7441 ctgccaagtg cgtgtgcgac cgccgctatc atctgcagct gctggcgccg cgtctgaaag
     7501 cccggcaacc cagcttgcag agctgacatg cattggcgga tcagagcctg ttcttcgtcg
     7561 gtgatcattg agcaagcacg gtagcaaacg cgcagccacg atcaactgtc cggcttgatg
     7621 ctggtcgacc atgatcaccc gtgccaaatc gtcgtggtcg gtcagtgcga cgttgtgaca
     7681 gatgagtggc agccaccgac ccgttg
//
LOCUS       DAOQNN010000127         7700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4920_length_7700_cov_8.734729, whole genome shotgun sequence.
ACCESSION   DAOQNN010000127 DAOQNN010000000
VERSION     DAOQNN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4920_length_7700_cov_8.734729"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKV97_08380"
     CDS             complement(<1..194)
                     /locus_tag="PKV97_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05929.1"
                     /translation="MSATSSGLKSKLRETLKQEDAALAERSRAVAKPAAAQPRVGKPD
                     AAEPAATATPTTAAQALQPTV"
     gene            complement(191..856)
                     /gene="parA"
                     /locus_tag="PKV97_08385"
     CDS             complement(191..856)
                     /gene="parA"
                     /locus_tag="PKV97_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HNB05930.1"
                     /translation="MSAPTLIALISQKGGSGKTTVAMQLAAGLALEGYRVALADLDPQ
                     ESASRWAESAPADAPFPARVVRLSGSADEMGKTLRPVANKVDVVVMDCPPSIEHAHTM
                     SALALCDIALVPVVPGPTDLWATRGIERLILECKRSRPGLRGALLPNRVTRTALSADV
                     LEVLRDFTLPVLDAALSQRSAYALGAVRGTSVFGLGRSAAPAQEEVARLVAAVVKLIE
                     ERG"
     gene            1096..1635
                     /locus_tag="PKV97_08390"
     CDS             1096..1635
                     /locus_tag="PKV97_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNB05931.1"
                     /translation="MAQEIERRFLVRDVRILDGRRGEPIIQGYLAKEVGSMSTRVRIR
                     GERAFLTLKSPKQGFSRDEFEYPIPVDDARQMIVRHCAGRVVRKTRYLVDHAHHVFEV
                     DVFQGHHAGLVVAEVELPHEETPLCLPHWIGDEITHDSQYGNFTLAEQAALLHGTPHR
                     THAAPTPVVVAPRSLPAAH"
     gene            complement(1678..3927)
                     /locus_tag="PKV97_08395"
     CDS             complement(1678..3927)
                     /locus_tag="PKV97_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB05932.1"
                     /translation="MDDVRNIRGTAIMRIVIVEDSPLLRDNLIRLFADHPELSVLGSA
                     AGEDAAVALIAAQRPDTVLLDLSLSQGSGLGVLRRLRATPGLAPRVLVLSSRPADPWE
                     ALCLDAGADAFLDKAGDPSVLLAQLASWMPPLPLDELRRRRRLQQLQVLDTRAEPALD
                     AIARLAARIAEAPVGVISLVDTHRQWFKARVGVEVQTTSRSVSFCAHALLGDGLFEVE
                     DATLDPRFADNPLVRGDPGIRFYAGMPLVMPGGEVVGTLCVIDSRPRRLGATAREALA
                     VLARSVTDELELRQRVGELEEEVARRHEAEARIMQLATRDALTGLPNRAALMDRLHQG
                     VRAAQRSGNLLAVLFLDLDRFKWINDTLGHDLGDALLQEMALRLSRAVRECDTVARLG
                     GDEFAIVLGDLWQVADAEAVAAKIVDVITRPLEVRGHALRVGGSVGVAVYPFQAHDEE
                     SLLRHADLAMYHAKESGGNRHQLFSEQMNARAVERMTVESELRAAIAAGQLELHYQPQ
                     LCLEDGRLAGMEALVRWRHPRLGLLAPGRFVPLAEDCGLIWELGLAVLELALAQLAAW
                     DRAGLEVPRIAVNVSPAQLRPELVERIVEILDRHGVAARRLEIELTESALTADGPAAN
                     GLLQALRRMGASIAIDDFGVGYSSLTLLRRLPISALKIDRSFVAELATNRQDAAIVEA
                     ILRMAHSVGLRTVAEGVEHAEQQAALGELGCQEVQGYLCARPLEAKAASAWLAGQHAG
                     GLVPSAAAA"
     gene            complement(4324..4965)
                     /locus_tag="PKV97_08400"
     CDS             complement(4324..4965)
                     /locus_tag="PKV97_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB05933.1"
                     /translation="MKLLIVDDHPVMRFGVRQLVTQRWPDAVIGEASCLAQALEQVRA
                     AEWELAVLDLSMPDTSGLEGLVRLHRAAPHTRLLVLSMHDEAAYAARALQLGAAGYLT
                     KEHATTELVAAIERVRGGQRYISSDLAARLADLLSGEAPARAPHETLSAQEYRVLLLI
                     AAGHGASEIAEIMHLSVKTVGTYRSRIIDKTGLANTAEMARYCSEHGLVDRGG"
     gene            complement(4962..6335)
                     /locus_tag="PKV97_08405"
     CDS             complement(4962..6335)
                     /locus_tag="PKV97_08405"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNB05934.1"
                     /translation="MSDLPRKPTGVLAATLLILFGLALFVALAWSSHASLRELEAVTD
                     ARSRSRNGLLLAAEAMSALKDVETGQRGYVLTGDEHYLGPFETGTRSLDETFEALRRT
                     LDDLPQARLETLWTLVEHRVALAERNIADRRAYAEENSPWKQRLDEGRDTMDRIRARF
                     AGIEAMLRAEIEARDRSLSALRAHALLLNVLLPAIGGALILAAWGLLQRERRRRVDAE
                     AALLAANARLEDTVAQRTVELQRALGEIEAFAHGLDRSIEDERRRLAREVHDQLGQLL
                     TALKMMVHRALQGAPGREQDWPQIEAVLGEGIATVRRISAALRPPLLDDLGLEAALGL
                     AGRQFAERTGLACEVALVDAGQLSADQANQLYRIAQEALTNVARHAQARRVRVEGVAV
                     DGEYRLAVEDDGVGMAPQARSSLGLLSMRERAALAGGELHVGSGAGGGVRIEARVPLI
                     ATGGEGG"
     gene            complement(6485..7432)
                     /locus_tag="PKV97_08410"
     CDS             complement(6485..7432)
                     /locus_tag="PKV97_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNB05935.1"
                     /translation="MSIDVLLPAALAFIMFSVGLALQGADFHRVFARPRALLIGLLGQ
                     LVLVPLAALAVVLAFDLPVLLGMGLMVLAACPGGASSGFLTHLARANAALSLSLTVIS
                     SLAALLTFPLLVKAVLAAFGEGVFGADGSVLAALPVGRLIGSVLVVTTVPIVAGMLLR
                     RRAPALTARAEPLVARVATLFFAAIVVATFVSHRHTILANLLSVGPATLVLNFVVMGL
                     GYGLVMLAGAERRDAVAVAMECGLQNAGLAIFVAIVLLRQPELAVGAVVYALTMNFGA
                     LGLVFVARRREGALAHDAKRVLRLPPGPPTGSRATPPRR"
     gene            complement(7442..>7700)
                     /locus_tag="PKV97_08415"
     CDS             complement(7442..>7700)
                     /locus_tag="PKV97_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HNB05936.1"
                     /translation="GAEGARRAFALLVLLPIPTLILIAASGAPGAILGLVGAPALAPL
                     VWRFGRAEGGPALNALLVDTARAGTRTGLLAAAGIVFVSSL"
BASE COUNT         1156 a         2868 c         2717 g          959 t
ORIGIN      
        1 accgtcggct gcagcgcctg cgcggcggtc gtcggcgtcg cggtggcggc aggctccgcg
       61 gcgtccggct tgcccacacg aggctgcgcc gcagccggct tcgcgaccgc ccggctgcgc
      121 tcggcgagcg cggcgtcctc ctgcttcagg gtctcgcgca gcttcgactt cagtccgctg
      181 ctggtggcgc tcatcctcgc tcctcgatca acttgacgac cgccgcgacc aggcgcgcca
      241 cctcttcctg cgcgggcgcg gcggaccgcc ccaggccgaa caccgaggtc ccgcgcaccg
      301 cgcccagcgc ataggcgctg cgctgcgaca gcgcggcgtc gagcaccggc agggtgaagt
      361 cgcgcagcac ctcgagtacg tccgccgaca gcgcggtgcg cgtcacgcgg ttgggcagca
      421 gcgcgccgcg caggccgggg cggctgcgct tgcactcgag gatcaggcgc tcgatgccgc
      481 gcgtcgccca caggtcggtc gggcccggca ccaccggcac cagggcgatg tcgcacagcg
      541 cgagcgcgct catcgtgtgc gcatgctcga tcgacggcgg gcagtccatg accaccacat
      601 cgaccttgtt ggccaccggt ctcaaggtct tgcccatctc gtcggcactg ccggacaggc
      661 gcaccacgcg ggcggggaag ggtgcatcgg cgggcgcgga ctcggcccag cgcgaggcgc
      721 tctcctgcgg atcgaggtcg gccagcgcga cgcggtagcc ctcgagggcg aggccggcgg
      781 cgagctgcat ggcgaccgtg gtcttgcccg agccgccctt ctgggaaatc aatgcgatca
      841 gggtgggtgc gctcatggtc catcccgtgg aggtgatggc cgacagtcta gccaatcggc
      901 cgcccgccgt ggcccgccag aggcgtcccg tgaacgcgcg gtgggagccg gcgtccggtc
      961 atgcgaccgc cctgggccgc aacaacgtcc tgcacgggaa ggtccgcaat cgaggccccg
     1021 agaaatattg caccgcaaca atcacgccgc aatcctttgc tacaattcga accttctgca
     1081 acagggtttc cagccatggc gcaagagatc gagcgcaggt tcctcgtccg cgacgtccgg
     1141 atcctcgacg gacgcagagg cgagcccatc atccagggct acctcgccaa ggaggtcggc
     1201 tccatgtcga cgcgagtccg catccgcggc gagcgcgcct tcctgacgct gaagtcgccc
     1261 aagcagggct tcagccgcga cgaattcgag tacccgatcc cggtggacga cgcccgccag
     1321 atgatcgtgc ggcactgcgc cgggcgggtg gtgcgcaaga cccgctacct ggtcgaccac
     1381 gcccaccacg tgttcgaggt cgatgtcttc cagggccatc acgccggcct cgtggtcgcc
     1441 gaggtcgaac tgccgcacga ggaaaccccg ctctgcctgc cgcactggat cggcgacgag
     1501 atcacccacg actcgcagta cggcaacttc accctcgccg aacaggccgc cctgctccac
     1561 ggtacgccgc accgcacgca cgcggcgccc accccggtcg tcgtcgcccc ccgcagcctg
     1621 cctgcagcgc actgaaccgg cggccgcagc cgcgttccga cgcaagccgg cgccccctca
     1681 cgccgccgcg gcgctcggca ccaacccgcc cgcatgctgt ccggcgagcc aggcgcttgc
     1741 cgccttcgcc tcgagcgggc gcgcgcacag atagccctgg acctcctggc agccgagctc
     1801 cccgagcgcc gcctgctgtt cggcatgctc cacgccctcg gccacggtgc gcaggcccac
     1861 gctgtgcgcc atgcgcagga tcgcctcgac gatggccgcg tcctggcggt tggtggcgag
     1921 ctcggcgacg aagctgcggt cgatcttgag cgccgagatc ggcagccggc gcagcagggt
     1981 cagcgaggag tagcccacgc cgaaatcgtc gatcgcgatc gaggcgccca tgcggcgcag
     2041 cgcctgcaac aggccgttgg cggccggtcc gtcggcggtg agcgcgctct cggtgagctc
     2101 gatctccagc cggcgcgcgg cgacgccgtg gcggtcgagg atctccacga tgcgctcgac
     2161 cagctccgga cgcagctgcg ccggggacac attcaccgcg atgcgcggca cctccagccc
     2221 ggcccggtcc caggccgcga gttgcgccag cgcgagttcc aggaccgcga gaccgagctc
     2281 ccagatcagc ccgcagtcct cggcgagcgg cacgaagcgg ccgggtgcga gcaggccgag
     2341 gcgcggatgg cgccagcgca ccagcgcctc catgccggca agccggccgt cttcgaggca
     2401 cagctgcggc tggtagtgca gctcgagctg gccggcggcg atggcggcgc gcagctccga
     2461 ctcgacggtc atgcgctcga ccgcgcgcgc gttcatctgc tcggagaaga gctggtggcg
     2521 gttcccgccc gactccttgg cgtggtacat cgccaggtcg gcgtggcgca gcagcgactc
     2581 ctcgtcgtgg gcctggaacg ggtacaccgc cacgcccacg ctgccgccca cgcgcagggc
     2641 atgcccgcgc acctccagcg gccgggtgat gacgtccacg atcttggccg ccaccgcctc
     2701 cgcatccgcg acctgccaga gatcgccgag cacgatcgcg aactcgtcgc cgccgaggcg
     2761 ggcgacggtg tcgcactcgc gcaccgcccg gctcaggcgc agcgccatct cctgcaacag
     2821 ggcgtcgccg aggtcgtggc cgagcgtgtc gttgatccac ttgaagcgat cgagatcgag
     2881 gaagagcaca gcgagcaggt taccgctgcg ctgcgcggcc cgtaccccct ggtgcagccg
     2941 gtccatcaac gcggcgcggt tgggcagccc ggtaagcgca tcgcgggtgg cgagttgcat
     3001 gatgcgcgcc tcggcctcgt ggcggcgggc gacctcctcc tcgagctcgc ccacacgctg
     3061 gcgcagctcg agctcgtcgg tgacgctgcg cgccagcacc gccagcgcct cgcgcgccgt
     3121 cgcacccagg cggcgcggcc gggagtcgat cacgcacagc gtccccacca cctcgccgcc
     3181 cggcatcacc agcggcatgc cggcataaaa gcggatcccc gggtcgccgc gcaccagcgg
     3241 gttgtcggcg aagcgcgggt ccagggtggc gtcctcgacc tcgaagaggc cgtcacccag
     3301 cagcgcatgg gcgcagaaac tcaccgaacg cgaggtcgtc tgcacttcga cgcccacgcg
     3361 cgccttgaac cactggcggt gggtgtccac caggctgatc accccgaccg gcgcctccgc
     3421 gatgcgtgcg gcgaggcggg cgatcgcgtc cagcgcgggc tcggcccggg tatcgagcac
     3481 ctgcagctgc tgcagccggc gacggcggcg cagctcgtcg agcggcagcg gcggcatcca
     3541 gctcgcgagc tgggcgagca gcacgctggg gtcgccggcc ttgtccagga aggcatccgc
     3601 gccggcgtcg agacacagcg cctcccaggg gtcggcaggc cggctgctca gcaccagcac
     3661 acgcggcgcc aggcccgggg tggcgcgcag ccggcgcagc acgccaaggc cgctgccctg
     3721 ggagagcgag aggtcgagca gcacggtgtc ggggcgctgc gccgcgatca gcgcgacggc
     3781 cgcgtcctcg ccggccgcgc tgccgagcac gctcagctcg ggatggtcgg caaagaggcg
     3841 gatcaggttg tcccgcagga gcggggaatc ttcgacgatg acgatgcgca tgatggcggt
     3901 tccacggatg tttcgaacgt catccatgtt ccgctcccgc cccctgcccc cccagcggcg
     3961 cctcgccgat gcaagggtcg gcggcccgcc gatcatgatg taaggcgaac acctacaaca
     4021 tcggcagcgg ggcaccgcag cctcacccga cgacaccgca gcctgcacgc cgcaggcggc
     4081 ggggcgggac ttgcggtcgc caacttcagg agcgccgagg cggagggcgt cgcgggggaa
     4141 acaaggcgac gcatgtccga ggagcgcagc gacgagtttg cgtcgccgcc cccgcgacgt
     4201 gctccgccga gggcagcccg gcgcgaaagc gacgggccgc gagtgcaggc ggcgaccgca
     4261 agtcccgccc caccgccggt tcgctcgcga cacctcgccc gaaaaacctc cccccgcgcg
     4321 ccctcagccc ccccgatcca ccagcccgtg ctcggaacag tagcgcgcca tctccgccgt
     4381 attggcgagt ccggtcttgt cgatgatgcg gctgcggtag gtgcccaccg tcttcaccga
     4441 cagatgcatg atctcggcga tctcgctcgc gccgtgcccg gcggcgatca gcagcagcac
     4501 gcggtattcc tgcgccgaca gcgtttcgtg cggcgcacgc gccggcgcct cgcccgagag
     4561 cagatcggcg aggcgggcgg cgaggtcgga gctgatgtag cgctggccgc cgcgcacgcg
     4621 ctcgatcgcg gcgaccagct cggtggtcgc gtgctccttg gtcaggtagc cggcggcacc
     4681 gagctgcagc gcacgcgccg cataagccgc ctcgtcgtgc atgctcagca ccagcagccg
     4741 ggtgtgcggt gcggcacggt gcagccgcac gagcccctcc agcccgctgg tgtcgggcat
     4801 cgacaggtcg agcacggcga gctcccattc cgccgcccgc acctgttcga gcgcctgcgc
     4861 caggcaggag gcctcgccga tcacggcgtc gggccagcgt tgggtcacga gctggcgcac
     4921 gccgaagcgc atcaccggat gatcgtcgac gatcagcagc ttcatcctcc ttcccccccg
     4981 gtcgcgatca gcggcaccct cgcctcgatc ctcaccccgc ccccggcgcc ggagccgacg
     5041 tgcagctcgc cgccggcgag cgccgcgcgc tcgcgcatgc tcagcagccc gagcgaggag
     5101 cgcgcctgcg gcgccatgcc gacgccgtcg tcctcgaccg ccaggcgata ctcgccgtcg
     5161 accgccacgc cctcgacccg cacccgacgg gcctgcgcgt ggcgggcgac gttggtgagc
     5221 gcttcctgtg cgatgcggta gagctgattg gcctggtcgg cgctcagctg cccggcatcg
     5281 acgagcgcga cctcgcaggc caggccggtg cgctcggcga actggcggcc ggcgaggccg
     5341 agcgccgcct ccaggccaag gtcgtcgagc agcggcgggc gcagcgccgc cgagatccgc
     5401 cgcacggtgg cgatgccctc gccgagcacg gcctcgatct gcggccagtc ctgctcccgt
     5461 ccgggcgcgc cctggagggc gcggtgcacc atcatcttga gcgcggtaag cagctgcccg
     5521 agctggtcat gcacctcgcg cgcgagccgc ctgcgctcgt cctcgatgct gcggtcgagg
     5581 ccgtgcgcga acgcctcgat ctcgccgagc gccctctgca actcaacggt gcgctgcgcg
     5641 accgtgtcct ccaggcgggc gttcgcggcg agcagcgccg cctccgcatc gacgcgccgg
     5701 cgccgctcgc gctgcagcag cccccacgcg gcgagaatca gcgcgccacc gattgccggc
     5761 agcagcacgt tgagaagcag cgcgtgcgcc cgcagtgccg acaagctgcg atcgcgcgcc
     5821 tcgatctcgg cgcgcagcat ggcctcgatg ccggcaaagc gcgcgcggat gcgatccatg
     5881 gtgtcgcggc cctcgtcgag gcgctgcttc cacggcgaat tttcttccgc gtaggcgcgg
     5941 cggtcggcga tgttgcgttc ggcgagcgcg acgcgatgct cgacgagggt ccacagggtc
     6001 tcgaggcggg cctgcggcag gtcgtcgagc gtccggcgca gcgcctcgaa ggtctcgtcc
     6061 aggctgcgcg tgccggtctc gaagggaccg aggtagtgct cgtcgccggt cagtacgtag
     6121 ccacgctgcc cggtctcgac gtccttcagc gccgacatcg cctcggcggc cagcagcagc
     6181 ccgttgcgcg agcgcgagcg cgcatccgtg accgcttcga gttcgcgcag gctggcgtgg
     6241 ctggaccagg ccagcgcgac aaaaagcgcc aggccgaaca ggatcaacag ggttgcggca
     6301 agcacgccgg tcggcttgcg gggcagatcg ctcatcgaca tcgagggccg gtcggggcgg
     6361 cggatctcag ccgccggtca tcatcggccc gatgttagcg gaatacggcg tgcagatgca
     6421 cgcgcgcccc gatcgaggcc gtatcgcact ccgcagagcc ggcgccggcg cggaacgtgg
     6481 gtgttcaacg ccgcggcggc gtggcgcgag agcccgtcgg aggccccggg ggcagccgca
     6541 ggactcgttt ggcgtcgtgc gcgagcgcgc cctcgcgccg gcgcgccacg aacaccaagc
     6601 cgagcgcgcc gaagttcatc gtcagcgcat acaccaccgc gccgacggcg agctcgggct
     6661 gacgcaacag cacgatggcg acgaagatcg ccagccccgc gttctgcagc ccgcactcca
     6721 tcgccaccgc gaccgcgtcc cgacgctcgg cgcccgccag catcaccagg ccgtagccca
     6781 ggcccatgac gacgaagttg agcaccaggg tcgccggtcc gaccgacagc aggttggcga
     6841 ggatggtgtg ccgatgcgac acgaaggtgg caaccacgat ggcggcgaag aacagcgtgg
     6901 cgacgcgggc gacgagcggc tcggcgcgcg ccgtgagcgc gggcgcccgg cgccgcagca
     6961 gcatcccggc gacgatcggc actgtcgtca ccaccagcac gctgccgatc aggcggccga
     7021 ccggcagcgc ggccagcacg ctgccgtccg caccgaagac gccctcgccg aatgcggcga
     7081 gcaccgcctt gaccagcagc gggaaggtca gcagcgcggc caggctgctg atcacggtaa
     7141 ggctcaagga cagcgccgcg ttggcgcgcg cgaggtgggt caggaagccc gagctcgccc
     7201 cgcccgggca ggccgccagc accatcaggc ccatgccgag gagcaccggc aggtcgaagg
     7261 ccagcaccac cgcgagcgcg gccagcggca ccagcacgag ctggccgagg aggccgatca
     7321 gcagcgcgcg cgggcgtgcg aacacgcggt ggaaatccgc cccctgcagc gccagcccga
     7381 ccgagaacat gatgaaggcg agggcggcgg ggagcaggac gtcgatgctc atcggtgcga
     7441 tctacaggct ggagacgaag acgatgccgg ccgcggcgag caagccggtg cgcgtaccgg
     7501 cgcgcgcggt gtcgaccagc agggcgttga gcgccggccc gccctcggca cgaccgaagc
     7561 gccacaccag cggcgccagc gcgggcgcgc cgacgaggcc gaggatcgcg cccggcgcgc
     7621 cgctcgcggc gatcaggatc agcgtcggga tgggcagcag caccagcagc gcgaaggcgc
     7681 ggcgcgcgcc ctccgcgccg
//
LOCUS       DAOQNN010000128         7681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4942_length_7681_cov_11.098348, whole genome shotgun sequence.
ACCESSION   DAOQNN010000128 DAOQNN010000000
VERSION     DAOQNN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4942_length_7681_cov_11.098348"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /gene="argH"
                     /locus_tag="PKV97_08420"
     CDS             <1..931
                     /gene="argH"
                     /locus_tag="PKV97_08420"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNB05937.1"
                     /translation="QVAQPVTFGHHLMAYFEMSRRDAERFADCRKRVNRLPLGSAALA
                     GTSYPIDREFVASELGFDEVCYNSLDAVSDRDFAIEFCAASALLMTHLSRLSEELILW
                     MSPRVGFIDLADRFCTGSSIMPQKKNPDVPELVRGKTGRVNGSLVALLTLMKGQPLAY
                     NKDNQEDKEPLFDTADTVIDTLRIYADMIGGIRVKAEAMRGALSQGYATATDLADYLV
                     KKGLPFRDAHEAVALAVRAAEVKGCDLPQFTLDELRIAMAHVPGAAERLGEDVFGVLT
                     VEGSLASRKHIGGTAPEQVRAAVARARIGLGQG"
     gene            948..2243
                     /locus_tag="PKV97_08425"
     CDS             948..2243
                     /locus_tag="PKV97_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNB05938.1"
                     /translation="MTTAAAERIGKYELLRRIGKGATSSVHLAWDPFREVEVAIKRLR
                     REILADPQRGRLLRQLFLNEAALAGKLAHPHIVRIHDALVTDEDAWLVMDHVPGGTLE
                     DIATPATLAPFGRVIEIVFKATRALDYAFHQGITHRDIKPANILLMAPGGSDIRISDF
                     GAALHTARETTQIAGVGSPAYMSPQQVREVPLDHRTDIYSLGVVMFQLLTGHLPFEQE
                     NSYSLLYSIAHDTPPPPSELRPEVPEALDAIVRRATEKDIERRYQSWSEFSHDLALAF
                     RNRSLALAPDRLGDTGKFEALRTMRFFRRFADAELWEVIGLSRFDRALAGTVIIKEGE
                     RGDAICFVLAGEARVSKQGRLLEVLNPGDCFGEIAVFSTISGVRSASVEAAAETELVT
                     IRARALERASETLRMHFYQAFLEVLATRLSQASARIANL"
     gene            2240..3163
                     /locus_tag="PKV97_08430"
     CDS             2240..3163
                     /locus_tag="PKV97_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HNB05939.1"
                     /translation="MNESPTPHPALPAVEAALRAAMGEQLRIVSAEPVSGGSIHTALR
                     LQDAGGARFFAKLAPAAQAPMFEAEADGLGAIAASGSLRTPAVIARGGDEAHAILVLE
                     WLDLQPLTSAADGARFAEALAALHRNVGEHFGWPRDNFIGRTPQANTERDNWARFFVE
                     QRLRPQFARARAQGFDIELQRQADRLLDRVPALFLDYRPPESLLHGDLWHGNAAVLAD
                     GTPVVFDPAVHRGDREADLAMSELFGGFPSAFYTAYRRAWPLHEDYEQRKPLYSLYHL
                     LNHLNLFGRAYLRESLRLATRLNEELARRRD"
     gene            3185..3937
                     /locus_tag="PKV97_08435"
     CDS             3185..3937
                     /locus_tag="PKV97_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05940.1"
                     /translation="MRRRFLHSAPERDLPAQHTYFEISDLRRFFDAGVIFATVWVRFI
                     GKTSGGRYNEIRHQRNQGLMMDRIRNNETVEQRRRVLKGALGASTVLTLGYGGSAAAA
                     SIGCVKNTYIEETPPANGNQFSWTDPAAMTGVDEWAWSKVDVWECSGNVGGIPDTDFE
                     GFSLDSGINWYRVPAVVGEAPTLIALATKKSSQSPAYPKTAWVLAYFDANGNFVGNYP
                     AITMRTEQNTPLEGSCLASVNPNAKSGFTYGG"
     gene            3944..4231
                     /locus_tag="PKV97_08440"
     CDS             3944..4231
                     /locus_tag="PKV97_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05941.1"
                     /translation="MQPTSPLGPLAWIERYCPSLDGQFLFLDPLRWDTHLLSAGAVIV
                     LREAALAIEAGCFEAFRAEVAANGGWPAGLERLAVALTALAERAAGTGTEA"
     gene            4243..5100
                     /locus_tag="PKV97_08445"
     CDS             4243..5100
                     /locus_tag="PKV97_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HNB05942.1"
                     /translation="MQRSIDLGAASVVVRSPLREFNAQLDLLYRDHSQAGDSGFADVR
                     VALLPGPPWRVWGPQVRLWSEASCPFDPYPLGSALPLFEWGTNWLLAQRLNAHVLLHA
                     GVVAREDRALILPAAPGSGKSTLTCALHLAGWRFLSDEFGVLDTESGLLHPLLKPAAL
                     KNRSIDIIGGQPGAVLGPVFAGTRKGDVAHFAPDRASVAARRRPVTPALVVFPRYREG
                     AALRCEPVRRAEAAMRLGLNSFNYRKLGPVGFDAILRLTRTAPAWEIEYGALADALPC
                     IEQLFGKAA"
     gene            5097..6200
                     /locus_tag="PKV97_08450"
     CDS             5097..6200
                     /locus_tag="PKV97_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNB05943.1"
                     /translation="MITHPAADCALLLRGLHEALEIPEARWDRVLRLARMTGLHGRLA
                     AANADNPTVSERVRRHLLSATRMADFSARMLDAELRDVAPLCGGDFPVIALKGGAYAL
                     QALGFARGRFVSDIDLLVPARALRTMEARLHAAGWESAALDPYDERYYRDWSHETPPL
                     RFPGHFLELDLHHAITPVTGRRHFDPAPLFDESLPIAGSRFRVLSARDQVLHACLHCF
                     QDGDLALRVREIADIDGLLRSFAGTTGFWPGLVERANALGLGRPLWYGLHFARSWMQT
                     PVPEAVLSALPGPAAVLRRPMEACISLALLPPEPDRPPGLSVRFARRLALARYHLLRM
                     PLHMLLPHLARKAWLRTRRRTPDGANENAGGGP"
     gene            complement(6248..6577)
                     /gene="grxD"
                     /locus_tag="PKV97_08455"
     CDS             complement(6248..6577)
                     /gene="grxD"
                     /locus_tag="PKV97_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNB05944.1"
                     /translation="MDIQDVIRDQVTNNGVVLYMKGTPQFPQCGFSSTAVQILKGCGV
                     REVLAVNVLADPEIRQGIKEFSNWPTIPQLYVKGEFVGGSDIMREMYDSGELQQMLKD
                     AGLVGED"
     gene            complement(6642..7511)
                     /gene="prmC"
                     /locus_tag="PKV97_08460"
     CDS             complement(6642..7511)
                     /gene="prmC"
                     /locus_tag="PKV97_08460"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNB05945.1"
                     /translation="MSPAAPSADGIDIAGALAWARARIDQMDARVLLRHVLQCPAARL
                     VAWPEQKLAAEDWAEYRALVERRAAGEPVAYLTGTREFYGREFLVTPAVLIPRPETEL
                     LVELALAHFPGRRGLRVLDLGTGSGALAVTLALELEAAEVVALDRSREALWVAMANAA
                     RLGASVSFVQSDWFGALGDEHFELIVSNPPYVAAGDPHLEQGDVRFEPRGALAAGPQG
                     LDDLAEIVAGAPARLVDGGWLFLEHGYDQAASARGLLADAGFAAIASWADLAGIERVS
                     GGRWLGRATRDSR"
     gene            complement(7508..>7681)
                     /gene="prfA"
                     /locus_tag="PKV97_08465"
     CDS             complement(7508..>7681)
                     /gene="prfA"
                     /locus_tag="PKV97_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311477.1"
                     /note="recognizes the termination signals UAG and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF1; this protein is
                     similar to release factor 2; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNB05946.1"
                     /translation="SERIRTYNFPQGRVTDHRINLTLYKLDAVMQGELDEIVDALTLE
                     HQADLLAALGEDA"
BASE COUNT         1150 a         2820 c         2565 g         1146 t
ORIGIN      
        1 gcaggtggcg cagccggtca ccttcggcca ccacctgatg gcctacttcg agatgagccg
       61 gcgcgacgcc gagcgcttcg ccgactgccg caagcgcgtc aatcgcctgc cgctcggctc
      121 ggccgcgctc gccggcacca gctacccgat cgaccgcgag ttcgtcgctt ccgagctcgg
      181 cttcgacgaa gtctgctaca actcgctcga cgccgtcagc gaccgcgact tcgcgatcga
      241 gttctgcgcc gccagcgcgc tgctgatgac ccacctgtcg cgcctgtccg aggagctgat
      301 cctgtggatg agcccgcgcg tgggcttcat cgacctcgcc gaccgcttct gcaccggcag
      361 ctcgatcatg ccgcagaaga agaaccccga cgtgcccgag ctggtgcgcg gcaagaccgg
      421 ccgcgtcaat ggcagcctgg tcgccctgct cacgctgatg aagggccagc cgctcgcgta
      481 caacaaggac aaccaggaag acaaggagcc cttgttcgac accgccgaca cggtcatcga
      541 cacgcttcgc atctacgccg acatgatcgg cggcatccgc gtcaaggcag aggccatgcg
      601 cggcgcgctc agccagggct atgccaccgc caccgacctc gccgactacc tggtgaagaa
      661 gggcctgccc ttccgcgacg cccacgaggc cgtggcgctg gcggtgcgcg cagccgaggt
      721 caagggctgc gacctgccgc agttcaccct cgacgagctg cgcatcgcca tggcccacgt
      781 gcccggcgcc gccgagcgcc tcggtgagga cgtcttcggc gtcctcaccg tcgaaggctc
      841 gctggcctcg cgcaagcaca tcggcggcac cgcgcccgag caggtgcgcg ccgcggtggc
      901 gcgcgcccgc atcggcctcg gccaaggctg agcccgatcc gacgcccatg accacagcgg
      961 cggccgagcg catcggcaag tacgagcttc tccgccgcat cggcaagggc gccaccagca
     1021 gcgtccatct cgcctgggac ccgttccgcg aggtcgaggt ggcgatcaag cgcctgcgcc
     1081 gcgagatcct cgccgatccg cagcgcggcc gcctgctgcg tcagctcttc ctcaacgagg
     1141 ccgcgctcgc cggcaagctc gcccacccgc acatcgtgcg catccacgat gcgctggtca
     1201 ccgacgagga tgcctggctg gtgatggacc acgtgccggg cggcacgctc gaggacatcg
     1261 cgacgccggc gacgctggcg cccttcgggc gcgtcatcga gatcgtcttc aaggccacgc
     1321 gcgcgctcga ctacgccttc caccagggca tcacgcaccg cgacatcaag ccggccaaca
     1381 tcctgctgat ggcgcccggc ggcagcgaca tccgcatctc cgacttcggc gccgcgctgc
     1441 acaccgcgcg cgagaccacg cagatcgccg gcgtcggctc gcccgcctac atgtcgccgc
     1501 agcaggtgcg cgaggtgccg ctcgaccacc gcaccgacat ctactcgctc ggcgtggtga
     1561 tgttccagct cctcaccggc cacctgccct tcgagcagga gaacagctac agcctgctct
     1621 acagcatcgc acacgacacc ccgccgccgc cctccgagct gcgccccgag gtacccgagg
     1681 cgctcgacgc gatcgtgcgc cgcgccaccg agaaggacat cgagcgccgc taccagagct
     1741 ggagcgagtt ctcccacgac ctcgcgctcg ccttccgcaa ccgcagcctc gcgctcgccc
     1801 cggaccgcct cggcgacacc ggcaagttcg aggcgctgcg cacgatgcgc ttcttccgcc
     1861 gcttcgccga cgccgagctg tgggaggtga tcgggctgtc gcgcttcgac cgcgcgctcg
     1921 cgggcacggt gatcatcaag gagggcgagc gcggcgacgc catctgcttc gtgctcgccg
     1981 gcgaggcgcg ggtgagcaag caggggcgcc tgctcgaggt gctcaacccg ggcgactgct
     2041 tcggcgagat cgcggtgttc tccaccatct ccggcgtgcg ctcggcctcg gtcgaggccg
     2101 ccgcggaaac cgagctggtg acgattcgcg cgcgcgcgct cgagcgcgcc agcgaaaccc
     2161 tgcgcatgca tttctaccag gctttccttg aggtgctggc gacgcgcctg tcgcaagcca
     2221 gtgcccgcat cgccaatcta tgaacgaatc gccgacgccc caccccgccc ttcccgccgt
     2281 cgaggccgcc ctgcgcgccg ccatgggcga gcagctgcgc atcgtctccg ccgagccggt
     2341 gagcggcggc agcatccaca ccgcgctgcg cctccaggac gccggtggcg cacgtttctt
     2401 cgccaagctc gcccctgccg cccaggcgcc gatgttcgag gccgaggccg acggcctcgg
     2461 cgcgatcgcg gcaagcggca gcctgcgcac cccggcggtg atcgcgcgcg ggggggacga
     2521 ggcccacgcc atcctggtgc tggaatggct cgacctgcag ccgctgacca gcgcggccga
     2581 cggcgcccgc ttcgccgagg cgctcgcggc gctgcaccgc aacgtcggcg agcacttcgg
     2641 ctggccgcgc gacaacttca tcgggcgcac gccgcaggca aacaccgagc gcgacaactg
     2701 ggcgcgcttc ttcgtcgagc agcgcctgcg tccgcagttc gcgcgcgccc gcgcgcaggg
     2761 cttcgacatc gagctgcagc gccaggccga ccgcctcctc gaccgtgtcc ccgcgctttt
     2821 cctcgactac cgcccgcccg agagcctgct ccacggcgac ctctggcacg gcaacgcggc
     2881 ggtgctcgcc gacggcacgc cggtggtctt cgatcccgcg gtgcaccgcg gcgaccgcga
     2941 agccgacctc gccatgagcg agctcttcgg tggtttcccc tccgccttct acaccgccta
     3001 ccggcgcgcc tggccgctgc acgaggacta cgagcagcgc aagccgctgt acagcctcta
     3061 tcacctgctc aaccacctca acctgttcgg ccgcgcctac ctgcgcgagt ccctccggct
     3121 ggccacgcgg ctgaacgagg agttggcgcg gcgcagggac tgaaacgcct cgaagcagga
     3181 gcggatgcgt cggcgttttt tacactcggc cccggagcgc gatctgcccg cacagcacac
     3241 ctattttgaa atcagcgact tgcgtcgatt tttcgacgcg ggcgtgatat ttgctacggt
     3301 ctgggtaagg ttcatcggaa agacgtcggg cggcagatat aatgaaatca ggcaccagcg
     3361 gaatcaggga ttgatgatgg acaggatccg gaacaacgag acggtcgagc agcgaaggcg
     3421 agtcctgaag ggcgcgctgg gcgcatccac cgtgctcacg ctgggatacg gcggtagcgc
     3481 ggctgcggca tcgatcggat gcgtgaagaa cacgtacatt gaagaaaccc cgcctgcgaa
     3541 cggtaaccag ttttcgtgga ccgacccggc ggccatgacc ggggttgacg agtgggcctg
     3601 gagcaaggtt gacgtgtggg aatgcagcgg gaacgtgggg ggcattccgg atacggactt
     3661 cgagggcttc tcgctcgaca gcggcatcaa ttggtaccgc gtgccggcgg tggtcggcga
     3721 agctccgacg ctgatcgcct tggcgacgaa gaagagctcg cagtcgcccg cgtacccgaa
     3781 gaccgcttgg gttttggcgt acttcgacgc gaacggcaac ttcgtcggca actaccccgc
     3841 gatcaccatg cgcaccgagc agaatacccc tctggagggt tcctgcctcg cgtcggtgaa
     3901 tccgaatgcg aagagtggct tcacctacgg cggctgaacg ccgatgcagc cgacctcgcc
     3961 gctggggccc ctggcctgga tcgaacgcta ctgcccgtcc ctcgacgggc agtttctttt
     4021 tctcgatccg ctgcgctggg atacccatct gctgagcgcg ggcgccgtga tcgtgctgcg
     4081 cgaggcggcg ctcgccatcg aagccggatg tttcgaggcc ttccgcgccg aggtcgccgc
     4141 gaacggcggc tggccggcgg gactggaacg cctggcggtc gcgctgaccg cgctcgccga
     4201 gcgcgctgcg ggcacgggga ccgaggcttg aaggccggcg tgatgcagcg ctcgatcgac
     4261 ctcggtgcgg ccagcgtggt ggtgcgctcg ccgcttcgcg agttcaatgc ccagctcgac
     4321 ctgctctacc gcgaccattc gcaggcaggg gacagcggct tcgcggacgt gcgcgtggcg
     4381 ctgctccccg ggccgccttg gcgcgtgtgg ggcccgcagg tacgcctgtg gtcggaggcc
     4441 tcctgcccct tcgaccccta cccgctcggc agcgccctgc ccctattcga atggggcacc
     4501 aactggctgc tggcgcagcg cctcaacgcg catgtgctgc tgcatgcagg cgtggtcgcg
     4561 cgcgaggacc gtgccctgat ccttcccgcc gcaccgggct cgggcaagag cacgctgacc
     4621 tgcgcccttc acctcgcagg ctggcgcttc ctctcggacg agttcggggt gctggatacc
     4681 gaatccggac tgctgcaccc cctgctcaag cccgcagcgc tgaagaatcg ctcgatcgac
     4741 atcatcggcg gccagccggg cgcggtcctc ggccccgtct ttgccggtac gcgcaagggc
     4801 gacgtggcgc acttcgcacc cgaccgtgcg agcgtcgcag cacgccgccg gccggtcaca
     4861 cccgcgctcg tggtgttccc gagatatcgc gaaggcgcag ccctgcgctg cgaaccggtg
     4921 cgcagggcgg aggccgcgat gcggctgggg ctcaacagtt tcaattaccg caagctcggc
     4981 ccggtcggct tcgacgccat cctccgcctc acgcgcacgg cccccgcctg ggagatcgag
     5041 tatggcgcgc tcgccgacgc cctcccctgc atcgagcagc tattcggaaa agccgcatga
     5101 tcacgcatcc ggcggccgac tgtgccctgc tgctgcgcgg ccttcacgaa gccctcgaga
     5161 tccccgaggc gcgctgggat cgggtgctgc ggctcgcgcg catgaccggc ctgcatggcc
     5221 gcctcgccgc cgccaacgcg gacaatccga ccgtgtcgga gcgggtccgc cgccacctgc
     5281 tgtcggcgac gaggatggcg gacttcagcg cgcgcatgct cgacgccgag ctgcgcgacg
     5341 tggccccgct gtgcggcggc gatttcccgg tgatcgccct gaagggcggc gcctacgcac
     5401 tgcaggcgct cggcttcgcc cgcggccgct tcgtctccga catcgacctg ctggtgcccg
     5461 cacgcgccct gcgcaccatg gaggcgaggc tgcacgccgc gggctgggag agcgcggcgc
     5521 tggaccccta cgatgaacgc tactaccgcg actggagcca cgagacgccc cccctgcgct
     5581 tcccgggcca tttcctcgaa ctcgacctgc atcacgcgat cacgccggtg accgggcggc
     5641 gccatttcga tccggcgccg ctgttcgacg agagcttgcc gatcgccggc agccgcttcc
     5701 gcgtgctgtc ggcgcgggac caggtgctgc atgcctgcct gcattgcttc caggacggcg
     5761 atctcgccct gcgcgtgcgc gagatcgccg acatcgacgg cctgctgcgc agcttcgccg
     5821 gcacgacggg cttctggccg ggcctcgtcg agcgtgccaa tgcgctcgga ctgggccgtc
     5881 cgctctggta tgggctgcat ttcgcgcgca gctggatgca gacgccggtc cccgaggcgg
     5941 tcctgagcgc ccttcccggc cccgccgccg tactccgccg gccgatggag gcctgcatct
     6001 cgctcgccct gctcccgccc gagcccgatc ggccacccgg cctgtcggtg cgattcgcgc
     6061 ggcggcttgc actcgcgcgc tatcacctgc tgcgcatgcc cctgcacatg ctcctgcccc
     6121 acctcgcgcg caaggcctgg ttgcgcacac gccgccgcac acccgacggt gcgaatgaaa
     6181 acgccggcgg cggaccctga aaacgggtcg gcggccggcg ttccacgacg aaggccgtcg
     6241 cgcgggatca gtcctcgccg acgagtccgg cgtccttgag catctgctgc agttcgccgc
     6301 tgtcgtacat ctcgcgcatg atgtcgctgc caccgacgaa ttcgcccttg acgtagagct
     6361 gcgggatggt gggccagttg gagaattcct tgatgccctg gcggatctcg gggtcggcaa
     6421 gcacgttgac cgcgagcacc tcgcgcacgc cgcagccctt gaggatctgc accgcggtgg
     6481 aggagaagcc gcactgcggg aactggggcg tgcccttcat gtagagcacc acgccgttgt
     6541 tggtgacctg atcgcggatg acgtcctgga tgtccattct ggagacctga aaaagttgag
     6601 ttaaacagtc gggaaataat aggcgttgcg cgccgccagc gtcaacgcga atcgcgcgtt
     6661 gcgcgcccca gccagcgccc ccccgagacg cgctcgatgc cggcgaggtc ggcccacgag
     6721 gcgatcgcgg caaagccggc gtcggcgagc aggccgcgcg ccgacgccgc ctggtcgtag
     6781 ccgtgctcga ggaagagcca gccgccatcg accaggcgtg ccggcgcgcc ggcgacgatc
     6841 tcggcgaggt cgtccaggcc ctgcggcccg gcggcgagcg cgccacgcgg ctcgaagcgc
     6901 acgtcgccct gctcgaggtg cgggtcgccc gcagccacat agggcggatt ggacacgatg
     6961 agctcgaagt gctcgtcacc gagcgcgccg aaccagtcgc tctgcacgaa ggagacgctc
     7021 gcgcccagcc tggcggcgtt ggccatcgcc acccacagcg cctcgcgcga gcggtcgagc
     7081 gcgaccacct cggccgcctc gagctcgagc gccagggtca ccgcgagcgc cccgctgccg
     7141 gtgccgaggt cgagcacgcg cagcccgcgc cgtccgggaa agtgcgcgag cgcaagctcg
     7201 accagcagct cggtctcggg gcgcgggatc agcaccgccg gggtgacgag gaactctcgc
     7261 ccgtagaact cgcgcgtgcc ggtgagatag gccaccggct cgccggcggc gcggcgctcg
     7321 acgagggcgc ggtactcggc ccagtcctcc gcagcaagct tctgctccgg ccacgccacc
     7381 agacgcgcgg ccgggcactg cagcacgtgg cgcagcagca cgcgcgcgtc catctggtcg
     7441 atgcgcgcgc gtgcccaggc gagcgcgccg gcgatgtcga tcccgtcggc ggacggcgcg
     7501 gcgggactca tgcatcctcc ccgagcgcgg cgagcaggtc ggcctggtgc tcgagggtga
     7561 gcgcgtcgac gatctcgtcg agctcgccct gcatcaccgc atcgagcttg tagagggtga
     7621 ggttgatgcg gtggtcggtc acccgcccct gcgggaagtt gtaggtgcgg atgcgctcgg
     7681 a
//
LOCUS       DAOQNN010000129         7673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4949_length_7673_cov_9.407062, whole genome shotgun sequence.
ACCESSION   DAOQNN010000129 DAOQNN010000000
VERSION     DAOQNN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4949_length_7673_cov_9.407062"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PKV97_08470"
     CDS             complement(<1..830)
                     /locus_tag="PKV97_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB05947.1"
                     /translation="MDAIFVFEQLLNGLGYGLMLFLIAAGLTLVFGIMDTMNLAHGSL
                     YMAGAYVAARVHEASGAFALAVLVAVGVTVAIAAVAEFLVIRRLYRRDHLVQVLATFG
                     IILIADDAVKAIWGVAPLLASTPAALAGPVALLEGLPYPSYRLLLIGAGLTVALGLHL
                     LVNKTRIGMLVRAGASNRQMAEHMGVRVGGVFAFVFALGAALAALAGALMAPISAVQI
                     GMGEAILIPALVVIVIGGIGSVRGAFVAALLVGLVDTAGRAFLPPALRAVLPPAVAAD
                     L"
     gene            complement(1030..2202)
                     /locus_tag="PKV97_08475"
     CDS             complement(1030..2202)
                     /locus_tag="PKV97_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB05948.1"
                     /translation="MLQMKKLSVAFALCGAAALNATAHAADIKVGIALDLSGPFSALG
                     AEARDGFELAIEKLGGKLGGQPAEFIRNDFGGNPEQANQLVARFLQRDGIHFFTGPIG
                     SNAALAVGPALFAAKVPYLSANPGPSAYAGEKCNPWFFAQYQNDTYDEAAGKVANEKG
                     YKNVLLIAPNYPAGKDHLNGFKRLYKGAVKDEIYTKLGQIDYAAEIAQIRAAKPDAVF
                     FFLPGAMGINFVKQFVGGGLEGVPLIAPGFSADEDVIQAVGEPMLGVLNTAHWTHDLQ
                     NPANAAFVSAFRQKYAGRYPSVYAAMAYDTLMAMDAAVRDVGGRIDDRDAVLKALKNP
                     SFKSLRGEFQYGANNFPVQNYYLREVGKDAEGRITNKLVGTTLEKHQDAYVGACRM"
     gene            2733..3335
                     /gene="ruvA"
                     /locus_tag="PKV97_08480"
     CDS             2733..3335
                     /gene="ruvA"
                     /locus_tag="PKV97_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNB05949.1"
                     /translation="MIGRITGTLLEKNPPQILVDVHGVGYEIDVPMSTFYNLPATGGT
                     VSLHTHFVVREDGHFLFGFATEEERATFRQLLKVSGVGARMALAVLSGLSVNDLAQAV
                     ALQEAGRLVKIPGIGKKTAERLLLELRDKLGKALPSMTGARLAAAAPGAAPDAKSDIL
                     NALLALGYNEREALSAMKGLAEDVGVSDGIRQALKALSKA"
     gene            3400..4455
                     /gene="ruvB"
                     /locus_tag="PKV97_08485"
     CDS             3400..4455
                     /gene="ruvB"
                     /locus_tag="PKV97_08485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNB05950.1"
                     /translation="MIETDKLKAAAPAERLISAAPADRQEDAIERALRPKRLAEYVGQ
                     VKIREQLEIFIQAARNRSEALDHVLLFGPPGLGKTTLAHIVAAEMGVNMRQTSGPVLE
                     RAGDLAALLTNLEPHDVLFIDEIHRLSPVVEEILYPALEDFQIDIMIGEGPAARSVKL
                     DLPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYTADELAFIVGRSARLLNVEIEDNG
                     ALEIARRARGTPRIANRLLRRVRDYAEVKAGGRITAAVADAALRMLDVDNLGLDLMDR
                     KLLSAMLEKFGGGPVGLDNIAAAIGESTDTIEDVIEPYLIQQGYLQRTPRGRMATQSI
                     WQHFGLVPPRIGGDLFQ"
     gene            4471..4824
                     /locus_tag="PKV97_08490"
     CDS             4471..4824
                     /locus_tag="PKV97_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05951.1"
                     /translation="MRSLQVVMLMLCVLGGMFLLVRAPEFFMPAQWDPSIGRSFDPLA
                     SRLLGAGLLALAAAGAGYLHAMYYSMPRRLPGPAAQRRHFALLLAALVLIGAALARAE
                     VGPNPEYRAPLRTGS"
     gene            complement(4924..5436)
                     /locus_tag="PKV97_08495"
     CDS             complement(4924..5436)
                     /locus_tag="PKV97_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05952.1"
                     /translation="MSPTAHPEHPNAASARRTIKAVALVLARHEIRSLRRWFEDFDRD
                     ILLPILLGEIALHNIGALQNGVARRESTRPITATPSVPRDTRPADCGLKPCNAYSIAA
                     ATGLPRETVRRKIGRLIELGWICREDNGHLYITEIALEQFGSMLSSRALGELLETADH
                     VRTLLEDEHD"
     gene            complement(5667..6581)
                     /gene="xerC"
                     /locus_tag="PKV97_08500"
     CDS             complement(5667..6581)
                     /gene="xerC"
                     /locus_tag="PKV97_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNB05953.1"
                     /translation="MGPRRSAEAPAALPERMQAWLDWLATQRRVSPLTQTAYRAGLQA
                     LLRLAEEHPPESLQPADIRRFVARLHGEGQQGRSIARHLSAWRGFYRWLVREHGVRIN
                     PALGIRPPKSPSTLPRVLSPEQANALLDPEPQNLLEIRDIAMFELFYSSGLRVAELAG
                     IDIDSGLDLAEGLVTVTGKRQRQRTVPVGGKAIAALRHWLEIRAGLAAAGERALFVTR
                     DGHRLSTRGIASRLGTRAQALGLGVHVHPHMLRHSFASHLLQSSGDLRAVQELLGHAS
                     IRSTQIYTHLDFQHLAAVYDAAHPRARK"
     gene            complement(6586..7242)
                     /locus_tag="PKV97_08505"
     CDS             complement(6586..7242)
                     /locus_tag="PKV97_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HNB05954.1"
                     /translation="MNAEDVARYLREHPDFLNQHHELFSELTVPHPQHGGQAISLAER
                     QLHALRDKIRALELKLAELIRFGEENDDISTKVHRLSVALLQAGSAAAVRQGLADSLR
                     DDFAVPHVSLKLWGEVGGGVVHTEAFGVGEAARRQVENLRHPWCGVPESIEMTSWFGE
                     AAPHIRSLALMPLRVGGNCIGLLALGSAEPERFYAGMGTLYLGRIADLAAAALAARAG
                     "
     gene            complement(7395..>7673)
                     /locus_tag="PKV97_08510"
     CDS             complement(7395..>7673)
                     /locus_tag="PKV97_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNB05955.1"
                     /translation="RFPARVNAGFLQVVDAHEVRLRVFERGAGETLACGTGACAAVVA
                     GILRGLLETPVRVHTRGGELEIAWDGPGTPVRMTGPAVTVFEGEMELA"
BASE COUNT         1189 a         2697 c         2660 g         1127 t
ORIGIN      
        1 aggtcggccg ccaccgcggg cggcagcacc gcgcgcaggg ccggcggcag gaaggcgcgc
       61 ccggcggtgt ccaccaggcc gaccagcagc gcggccacga aggcgccgcg caccgagccg
      121 atgccgccga tgacgatcac caccagcgcc gggatcagga tcgcctcgcc catgccgatc
      181 tgcaccgcgc tgatcggcgc catcagcgcc cccgccagcg ccgcgagcgc agcgccgagc
      241 gcgaacacga aggcgaacac cccgcccacg cgcacgccca tgtgctccgc catctgccgg
      301 ttcgaggcgc cggcgcgcac cagcatgccg atgcgggtct tgttgaccag caggtggagc
      361 ccgagcgcca ccgtcagccc ggcgccgatc agcagcaggc ggtaggacgg atagggcagg
      421 ccttcgagga gcgcgaccgg cccggccagc gccgccgggg tcgaggccag cagcggcgcc
      481 acgccccaga tcgccttcac cgcgtcgtcg gcgatgagga tgatgccgaa ggtggcgagc
      541 acctggacga ggtggtcgcg ccgatacagc cggcggatga cgaggaactc cgccaccgcc
      601 gcgatcgcca ccgtcacgcc gaccgcgacc agcacggcga gcgcgaacgc gccgctcgcc
      661 tcatgcacgc gcgcggcgac gtaggcgccg gccatgtaga gcgagccgtg ggcgaggttc
      721 atggtatcca tgatgccgaa cacgagcgtg agcccggcgg cgatcaggaa cagcatcagc
      781 ccgtagccca ggccgttgag caactgctcg aagacgaaga tggcatccat cggatttcag
      841 cccggcgcgt ccgaagcctc tgtgtaggag cgacgccagt cgcgatccgc ccatggagca
      901 tcccgccgca ctcggggcag cagtgagctc caaatcatga agctgccgtt cggatgatgc
      961 atcgcggtga atcgcgactt gcgtcgctcc cacggcggcc ccatgccccg cgcgcgcgga
     1021 cctcgggtct cacatcctgc acgcgcccac gtaggcgtcc tggtgctttt cgagcgtggt
     1081 gccgaccagc ttgttggtga tgcggccctc ggcgtccttg ccgacctcgc gcagatagta
     1141 gttctgcacc gggaagttgt tggcgccgta ctggaattcg ccgcgcaggg acttgaagct
     1201 gggattcttg agcgccttca gcaccgcgtc gcggtcgtcg atcctgccgc cgacgtcgcg
     1261 caccgcggcg tccatcgcca tcagggtgtc gtaggccatc gccgcataca ccgacgggta
     1321 gcgcccggcg tacttctggc ggaaggcgga gacgaacgca gcgttggcgg ggttctgcag
     1381 gtcgtgcgtc cagtgcgcgg tgttgagcac gccgagcatc ggctcgccca ccgcctggat
     1441 cacgtcctcg tcggccgaga agccgggggc gatgagcggg acgccctcga ggccgccacc
     1501 gacgaactgc ttgacgaagt tgatccccat cgccccgggc aggaagaaga acaccgcgtc
     1561 cggcttggcg gcgcggatct gcgcgatctc ggcggcgtag tcgatctggc cgagcttggt
     1621 gtagatctcg tccttcaccg cgcccttgta caggcgcttg aagccattca gatgatcctt
     1681 gcccgccggg tagttgggcg cgatcagcag gacgttcttg tagcccttct cgttggcgac
     1741 cttgccggcg gcctcgtcgt aggtgtcgtt ctggtactgg gcgaagaacc aggggttgca
     1801 cttctcgccg gcgtaggcgc tcgggcccgg gttggccgac aggtagggca ccttggcggc
     1861 gaacagcgcc gggccgaccg cgagcgcggc gttggagccg atcggtccgg tgaagaagtg
     1921 gatgccgtcg cgctgcagga agcgggcgac gagctggttg gcctgctccg ggttgccacc
     1981 gaagtcgttg cggatgaact ccgccggctg gccgccgagc ttgccgccga gcttctcgat
     2041 cgccagctcg aaaccgtcgc gcgcctcggc accgagcgcc gagaagggac cggagaggtc
     2101 gagcgcgata ccgaccttga tgtcggcggc gtgggcggtg gcgttgaggg cggcggcgcc
     2161 gcacagggcg aacgcgacgg acagcttctt catctgcagc atggggtctc ctcgattgtt
     2221 ctctttgccg ctcgcaacgc cggcgtcgga gcgccgcgcc acgcgcgaag gccgccgatt
     2281 ctaccccagc ccggccgcgc ctcgcgccgc cggccgcccc tggaggaacg acatggcttc
     2341 ggcaaactcc gcaggatcga tgcaggtcgc gatcggctcc tcgaccgggc atcgacacga
     2401 atctcccggg ccgcactcgc gactcgcgtc gcgcctacaa gaacggcggg ttctccaacg
     2461 cgcccgcagg agcgacgcaa gtcgcgatcg gcccctcgat tgggcatcga tacgaatctc
     2521 ccgagccgca ctcgcgactc gcgtcgctcc tacaagaacg gcgggttctc cgacgcgccc
     2581 gtaggagcga cgcgagtcgc gataaggcga tgaatggagc ggtagcgccg cacgaaacgc
     2641 cgcaccccgg cccgcgctgc tgtatgaaac cgcaggccgc acgcctgcgg cctgctagac
     2701 ttgcacatcc gcatttcgca cgcccgcccc tcatgatcgg acgcatcacc ggcaccctgc
     2761 tggaaaagaa cccgccgcag atcctcgtcg acgtgcatgg cgtcggctac gagatcgacg
     2821 tgccgatgag caccttctac aacctgcccg ccaccggcgg cacggtcagc ctgcacacgc
     2881 acttcgtggt gcgcgaggat ggccacttcc tgttcggctt cgccaccgag gaggagcgcg
     2941 ccaccttccg ccagctgctc aaggtctcgg gggtgggcgc gcgcatggcg cttgcggtgc
     3001 tgtccgggct gtcggtgaac gacctcgccc aggcggtggc gctgcaggag gccggccgcc
     3061 tcgtgaagat ccccggcatc ggcaagaaga ccgccgagcg cctgctgctc gagttgcgcg
     3121 acaagctcgg caaggcgctg cccagcatga cgggcgcgcg actcgccgcg gccgcaccgg
     3181 gcgcagcccc ggacgcgaag agcgacatcc tcaacgccct gctcgcgctc ggctacaacg
     3241 aacgcgaagc gctgtccgcg atgaagggtc tggccgagga cgtcggcgta tcggacggca
     3301 tccgccaggc gctgaaggcg ctgtcgaagg catgatgcgc atgcaccccg ctcgccacgc
     3361 cgtcggctgc cgccctgcaa gctcggccag gacacgccca tgatcgaaac cgacaagctc
     3421 aaggctgccg cccccgccga gcgcctgatc tccgccgccc ccgccgaccg ccaggaagac
     3481 gcgatcgagc gcgcgctgcg tcccaagcgc ctggccgagt acgtcggcca ggtgaagatc
     3541 cgcgaacagt tggagatctt catccaggcg gcgcgcaatc gcagcgaggc gctcgaccac
     3601 gtgctgctgt tcggcccgcc cgggctgggc aagaccacgc tcgcccacat cgtcgccgcc
     3661 gagatgggcg tgaacatgcg ccagacctcc ggcccggtgc tcgagcgcgc cggcgacctc
     3721 gccgcgctgc tcaccaacct cgaaccgcac gacgtgctct tcatcgacga gatccaccgc
     3781 ctgtcgccgg tggtcgagga gatcctctac ccggcgctgg aggacttcca gatcgacatc
     3841 atgatcggcg agggcccggc ggcacgctcg gtgaagctcg acctgccgcc cttcacgctg
     3901 gtcggcgcca ccacccgcgc cggcatgctc accaacccgc tgcgcgaccg cttcggcatc
     3961 gtgtcgcggc tggagttcta caccgcggac gagctcgcct tcatcgtcgg acgctcggcg
     4021 cgcctgctca atgtggaaat cgaggacaac ggcgcgctcg agatcgcccg ccgcgcgcgc
     4081 ggcacgccgc gcatcgccaa ccgcctgctg cgccgggtgc gcgactacgc cgaggtgaag
     4141 gcgggtggcc gcatcaccgc cgcggtcgcg gacgcggcgc tgcgcatgct ggacgtcgac
     4201 aacctgggcc tcgacctgat ggaccgcaag ctgctgtcgg cgatgctgga gaaattcggc
     4261 ggcggcccgg tcggcctgga caacatcgcc gcggcgatcg gcgaatcgac cgacaccatc
     4321 gaggacgtca tcgagcccta cctgatccag cagggctacc tgcagcgcac cccgcgcggg
     4381 cgcatggcga cgcagtcgat ctggcagcac ttcggcctcg tgccgccgcg catcggcggc
     4441 gacctcttcc agtaggcgac ggggccgacc gtgcgctcgc tgcaggtggt gatgctgatg
     4501 ctgtgcgtgc tgggcgggat gttcctgctt gtgcgagcgc ccgagttctt catgcccgca
     4561 cagtgggacc cctcgatcgg acgcagcttc gatccgctcg cctcgcgcct gctcggcgcc
     4621 gggctgctgg cgctcgccgc cgcgggagcg ggctatcttc acgcgatgta ctactcgatg
     4681 ccgcgccgcc tgcccggccc ggcagcgcag cgccgccact tcgccctgct gctcgccgcc
     4741 ctggtgctga tcggcgcggc gctggcgcgc gccgaggtgg ggccgaatcc ggaatatcga
     4801 gcgcccttgc gcacgggttc gtagggagca ctgcggccgc aaagccacac gttgcccggc
     4861 gcgaggctgc tgcggctcgc gtccctcctg cagaagcctg cacgcccctg cgcgcggcct
     4921 gcttcagtcg tgctcgtctt cgagcagggt gcggacgtgg tcggccgttt cgaggagctc
     4981 gccgagcgcg cgcgagctga gcatggagcc gaactgctcg agcgcgatct cggtgatgta
     5041 gaggtgtccg ttgtcttcgc gacagatcca gcccagctcg atcaggcgcc cgatcttgcg
     5101 ccgcaccgtc tcgcgcggca gaccggtggc ggcggcgatc gagtaggcgt tgcagggctt
     5161 gaggccgcaa tccgccgggc gcgtgtcgcg cggcaccgag ggcgtcgcgg tgatcggacg
     5221 ggtgctctcg cggcgcgcga caccgttctg caaggcgccg atattatgca gtgcgatctc
     5281 gccgagcagg atggggagca ggatgtcgcg gtcgaagtct tcgaaccagc gccgcaagct
     5341 gcggatctcg tggcgcgcga gcacgagcgc gaccgccttg atcgtgcggc gtgccgacgc
     5401 cgcattcggg tgttccgggt gcgctgttgg gctcattggg ctgtcatggc acgcttcacc
     5461 cttttttgtt gattccggga gctagcggcg ttttttagaa aattcttaaa ttaacagacg
     5521 ccatgagcat aacccaagcc gaacgggctc aatactcggg aatacccata atggggagag
     5581 tcacattgtg accccaaccg ccctcttctg ccgctgcgac tccccggatc ggggcgtgcg
     5641 cgccggggtg gcgagcgcgt gacggactac ttgcgcgcgc gcggatgtgc cgcgtcgtac
     5701 accgcggcga gatgctggaa atcgagatgc gtgtagatct gcgtgctgcg gatgctggcg
     5761 tggccgagca gttcctggac agcgcgaaga tcgccgctcg attgcagaag atgactcgca
     5821 aaactgtgcc gcagcatgtg cggatgcaca tgcacgccga gcccgagcgc ctgagcgcgc
     5881 gtgcccagcc ggctggcgat gccgcgggtg ctcagacggt ggccgtcgcg ggtcacgaac
     5941 agcgcgcgct cgcccgccgc agcgaggccg gcgcggatct ccagccagtg gcgcagggcc
     6001 gcgatcgcct tgccgcccac cggcacggtg cgctggcgct ggcgcttgcc ggtgaccgtg
     6061 accaagccct cggcgaggtc cagcccggaa tcgatgtcga ttccggcgag ttctgcaact
     6121 cgcagaccgg aagaataaaa aagctcgaac atcgcaatat cccgaatttc tagcaaattc
     6181 tgcggctctg gatcgagcag cgcgttcgcc tgctcgggcg agagcacgcg cggcagcgtc
     6241 gagggggact tgggcggacg gatgccgagc gccggattga tgcgcacgcc gtgctcgcgc
     6301 accaaccagc ggtagaagcc tcgccacgcc gacagatggc gggcgatcga gcgcccctgc
     6361 tggccctcgc catgcaggcg ggcgacgaag cggcggatgt cggcgggctg caaggattcg
     6421 ggcgggtgct cctcggcgag gcgcagcaag gcctgcaggc cggcgcggta ggcggtctgc
     6481 gtcagcggcg agacccggcg ctgggtggcg agccagtcca gccaggcctg catgcgctcg
     6541 ggcagcgcgg ccggcgcctc ggcggaccgc cgcgggccca tggattcagc ccgcgcgcgc
     6601 cgccagcgcc gcggcggcga ggtcggcgat gcggcccagg tagagcgtgc ccatgccggc
     6661 gtagaagcgc tcgggctcgg cgctgccgag cgcgagcagg ccgatgcagt tgccgccgac
     6721 gcgcagcggc atcaacgcca gcgaacggat gtgcggcgcg gcctcgccga accagctcgt
     6781 catctcgatg ctctcgggca ccccgcacca tgggtggcgc aggttctcga cctggcggcg
     6841 cgccgcttcg ccgacgccga aggcctcggt gtgcaccacc ccgcccccga cctctcccca
     6901 cagcttgagg ctgacgtgcg gcacggcgaa gtcgtcgcgc aggctgtcgg ccaggccctg
     6961 gcgcaccgcg gcggccgagc ccgcctgcag cagcgcgacc gacaggcggt gcaccttggt
     7021 gctgatgtcg tcgttctcct caccgaagcg gatcagctcg gcaagcttga gttcgagcgc
     7081 acggatcttg tcgcgcaggg catggagctg gcgctcggcg agcgagatcg cctggccgcc
     7141 atgctgcgga tgcggcacgg tgagctcgct gaagagctcg tgatgctggt tgaggaaatc
     7201 ggggtgttcg cgcaggtagc gtgcgacatc ttctgcattc atcgatgctg ctccaggcga
     7261 ggcggcggcc gccccgcgtc gatcgttcgt ggatggccgc cgcatccggc gcggtcggcc
     7321 gcgagtcggc cggcacgatg ccgcacaggc gcatcgcgtc cggcccggca aaatccggac
     7381 tcgggcgggc tccctcaggc gagctccatc tcgccctcga aaaccgtgac cgccggtccg
     7441 gtcatgcgca ccggcgtgcc cgggccgtcc caggcgatct ccagctcgcc gccgcgggta
     7501 tgcacccgca ccggcgtctc cagcaggccg cgcaggatgc ccgccaccac cgccgcgcag
     7561 gcgccggttc cgcaggccag ggtctcgccg gcgccacgct cgaacacgcg cagacgcacc
     7621 tcgtgcgcat cgaccacctg caggaagccg gcattgaccc gcgccgggaa gcg
//
LOCUS       DAOQNN010000130         7626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_4993_length_7626_cov_9.729758, whole genome shotgun sequence.
ACCESSION   DAOQNN010000130 DAOQNN010000000
VERSION     DAOQNN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4993_length_7626_cov_9.729758"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKV97_08515"
     CDS             <1..496
                     /locus_tag="PKV97_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNB05956.1"
                     /translation="REAYFRCASPEMVERMMHLDHKITLADNDLRKVTRMCEAAGVEV
                     RFPFLDDDLVEFSGRLTPRHKVDKGRLRVLFKDALRGFLPNETITKSKHGFGLPFGVW
                     LNSHGPLKSLADNALDSLSQRGIVRPAYLDALRKAHASGHASYYGVMIWILIELELWL
                     QHNA"
     gene            complement(598..1647)
                     /locus_tag="PKV97_08520"
     CDS             complement(598..1647)
                     /locus_tag="PKV97_08520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNB05957.1"
                     /translation="MDAVKHAVFPQVAGRMPSIDAARAIAIFAVLAIHCDPFIGRVYS
                     LGEVSAGELIDLAGRFAVPFFFVVSGYLLGERCRKSGRDLRVVSGFAGRLAGLVLFWY
                     AFYLLWTPDWNAAWEGGWIRLAYWRAAALFGDGWSLLAGPRAHLWFLPALLIGTLHVF
                     AVMRVKRRVPCLVYFALLYAMGLGAGAYADHPLLGPLSPGVPGGLMCAPLIIATGWWS
                     AGETVRMGRRVACFLFIGGTVATFVEAWTLLHTYGVPLEGHDFLLATPFQVWGLLNLL
                     RSYPSWAVGTPLPQWGRYTLGVYAGHVAVMEVLGAREGGGLLWEFLKTPLVYLLTLSV
                     VGLLARWPRVRPFFA"
     gene            complement(1829..2791)
                     /locus_tag="PKV97_08525"
     CDS             complement(1829..2791)
                     /locus_tag="PKV97_08525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB05958.1"
                     /translation="MSTSTRSPSVTVILPTHDRPRLLAEAIASVRAQTWENWDLIVVD
                     DASDPPASIPDDPRCALIRNVRSRGGAEAKNAGAFQARGDVLAFLDDDDLYAPSYLQR
                     AVAALAEDPELDVVFMGVSWFGERSEAGQRAYEQAMGALLSTLHPFSSPRGGVLAFEP
                     AELFRGLLRTVPMAFQRPVVRRSSFERIGPYQADILLWDCDWAIRAALQARCGLVTDG
                     LYMQRASGQGFSSKSDRQREHLRSNALMKQRLLEGKLEAEGQAAVRDALVELWFGAAW
                     SSSQGGDQPRALEYLWNSARIRLELGQLRLLARILARAIGLVRG"
     gene            complement(2823..4181)
                     /locus_tag="PKV97_08530"
     CDS             complement(2823..4181)
                     /locus_tag="PKV97_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HNB05959.1"
                     /translation="MRDLIVTLAVFGTLPFILRYPWIGILAWSWLSYMNPHRMAWGFS
                     TSMPFAFMVALATFVGILFSREKKEIVWSRETWLMLVFTGWMFLTTAFSWYPGLAWPQ
                     WDKVWRIMLMTYVTLMLIRDRDRVHWLVVVIAFSLAFYGVKGGIFVLTGGSGNNVRGP
                     GGSFIEDRNSIGLALLMTVPLLWYIRLQLKVAWQRLGLLAMSVLTLIAVIGTHSRGAL
                     VGLGAMGVFFLLKARNRLGVLIGIIPVVLVVFYVMPPEWFERMHTIQTYDEDGSAQGR
                     IYAWGNAVYIANSSLLGGGFRAVTGFGGTDSHSNWFGTLGEQGWPGLVMFVLLHIFTW
                     RSATQIIRMVKPHKELTWARDLAAMIQVSLIGYMSAGSFLGLQYFDLFYHLIVIIVVV
                     RELVRRAVGEIAEPRVQARGSSASGLERPLSSAGPTPPDKSHLPQPRVAGGRFASSRS
                     HR"
     gene            complement(4169..5542)
                     /locus_tag="PKV97_08535"
     CDS             complement(4169..5542)
                     /locus_tag="PKV97_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB05960.1"
                     /translation="MSGLYTRLVSGLLFPLHERLKHHDSVALRRGLEDSQWWPRERIE
                     ALQLKRLRALLVHARDHVPYYREGFARIGFDPAAIASLAELQRLPFLGKAEIRANTEA
                     LKADDAVGLARFNTGGSSGEPLIFFIGDRRVSHDVAAKWRATRWWGVDIGDPEIVVWG
                     SPIELGAQDRVRALRDRLFRTELLPAFEMSATKLDGFVARIRARRPKMLFGYPSALSH
                     IGLHAERRGIRMDDLGIEVAFCTSERLYDHQREAIERVFGCPVANGYGSRDAGFIAHQ
                     CPQGGMHLTAEDLIVEIVDGEGRVLPPGQAGEIVVTHLATGDFPFIRYRTGDVAVMDA
                     ATCACGRGLPMLREIQGRATDFVVARDGTVMHGLALIYVLRDLPQVAGFRIVQESLEH
                     TRVQVVPGAGFDADTVGRIEHGLAARLGEGVRIDVEQVAEIAPEKSGKYRYVVSKVDA
                     SEVSCAT"
     gene            complement(5559..7169)
                     /gene="xrtA"
                     /locus_tag="PKV97_08540"
     CDS             complement(5559..7169)
                     /gene="xrtA"
                     /locus_tag="PKV97_08540"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HNB05961.1"
                     /translation="MNMKGTAMLQSVRPGAMPTHLQATDARPATLGDPRTRRLHLAVL
                     ALGLLWLLFWYRDTFLAMVGIWDRSDTFAHGFVIAPISAWLVWRRRHFIDHVPIAPSL
                     LGICAGLVAGAAWLLGELASVDAVSQFAFVGMLVGFVWAVMGSAVVRTYAFPIGFLFF
                     MVPFGEFLFPTMMQWTADFIIWAVRASGVPVYAEGFLLVIPSGRWQVVEGCSGVRYLM
                     ASIVVGSLYAYLNYRSTQKRLLFVAASIVLPVFANWLRAYGIVMLGHLTDNRLAAGAD
                     HLIYGWVFFGIIILALFWIGSRWQEDEEDAPAMPVGSARSSGARTGGLGWLAVAVVAV
                     GLWVPVLGHLERQGEQGPVRFAALEGQGAWQRTEAGELPPWSPSYSGMRDTFRSTWRA
                     GSVPVGVYIGYYRDQGPGRELINSENRVLISKDPVWRMTAAGPVRVQLGDQAQAWRTL
                     EMASEHARMVVWYAYWIGGRWTTSDHLAKVYLALSRLRGEGDDSAVVMLHAPQGQGGH
                     AGTIAALEDFAREMGGPLQAMLDRTANP"
     gene            complement(7166..>7626)
                     /locus_tag="PKV97_08545"
     CDS             complement(7166..>7626)
                     /locus_tag="PKV97_08545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB05962.1"
                     /translation="ARLSIAGSGPEAAALAALAQELGIAERVRFTGRLDREQMAALYR
                     DADLMLNPSRVDNMPNAILEALASGLAVVTTDVGGIPFIVTQGHTAMLVPPDDPQAMA
                     DAAHKVLADTGLRAALVAAGCAEVQRYRWSSVRAQLLSAYADAIAAKRAT"
BASE COUNT         1443 a         2628 c         2488 g         1067 t
ORIGIN      
        1 gcgcgaagcc tacttccgtt gcgcctcgcc ggagatggtc gagcgcatga tgcacctcga
       61 ccacaagatc accctcgccg acaacgacct gcgcaaggtc acgcggatgt gcgaggccgc
      121 cggcgtggag gtgcgctttc catttctcga cgacgatctc gtcgagttct cgggccgact
      181 gacgccgcgg cacaaggtgg acaaggggcg gctgcgggtg ctgttcaagg acgcgctgcg
      241 gggctttctg ccgaacgaga cgatcaccaa gagcaagcac ggcttcggcc tgccgttcgg
      301 cgtctggctg aacagccatg gtccgctgaa gagcctcgcc gacaacgcgc tcgacagctt
      361 gtcccagcgc ggaatcgtcc gacccgcata cctcgacgct ctgcgcaagg cgcatgcatc
      421 cggacatgcg agctattacg gggtgatgat ctggatcctg atcgaactcg aactctggct
      481 gcagcacaac gcctgaaccg accgctccag tccggctccg acaagacgct gccccggctc
      541 cgaaatgcaa ggaccgggcc ggcgaacacc tgccagccag cggggcatcc gcacgcgcta
      601 ggcgaagaac ggcctcacgc gaggccacct ggcgagaagg ccaaccacgg acagggtgag
      661 cagatagacg agcggcgtct tgaggaactc ccagagcaaa ccgccgcctt cacgagcgcc
      721 gaggacctcc atcaccgcga catggccggc atacaccccc agcgtgtaac gtccccattg
      781 gggcaagggc gtccccaccg cccacgatgg atacgacctg agcaggttta acagccccca
      841 gacctgaaag ggcgtggcaa gcagaaaatc atgcccctcc agcgggacac cgtaggtgtg
      901 cagcagggtc caggcctcga caaaagtggc gaccgtgccg ccgatgaaga ggaagcaggc
      961 gacgcggcgc cccatgcgaa ccgtttcccc agccgaccac catccggtgg cgatgatcaa
     1021 cggtgcacac atgagccccc ccggcacacc cggagaaaga ggccccagca acgggtggtc
     1081 tgcatatgct ccggcgccga gccccatcgc atacagcaag gcgaaataga cgaggcaggg
     1141 gacgcgccgc ttcacgcgca tgaccgcaaa cacatgcagg gttccgatca gcagcgcggg
     1201 caggaaccat aaatgcgcac gcggcccggc gagcagactc cagccgtcgc caaacagcgc
     1261 cgcggcgcgc caataggcaa ggcggatcca tccgccttcc catgctgcgt tccagtcggg
     1321 ggtccagagc aggtagaacg cgtaccagaa gaggacaagc ccggccagcc gacccgcaaa
     1381 gccgctcacg acgcgcaggt cgcgcccgct cttcctgcat cgttcaccaa gcagatagcc
     1441 cgatacgaca aaaaagaagg ggacggcgaa ccgccccgcc agatcgatca gttcccctgc
     1501 ggaaacctct ccgagtgaat acacccgccc gatgaagggg tcgcagtgaa tcgccagcac
     1561 ggcaaagatg gcgatcgccc tcgcggcgtc gatactgggc atcctgccag cgacctgcgg
     1621 aaacactgca tgcttgacgg catccatgga aacggcatct cggaaaagag ggaggtggag
     1681 ccggaaggat catcggccag ggtggattgc aaggatccgc gcctcgaccg aggcagcccc
     1741 gattctcgat ccatcgaact catcgggaca ccctgcgttc gtgattcccc gaacatcacc
     1801 gcgcgaggca gcgctgcggg ctcccgggtc agccgcggac aagaccgatc gcccgtgcaa
     1861 ggatgcgggc cagaaggcgc aattgcccaa gttccagcct gatccttgca ctgttccaca
     1921 gatattcgag cgcccggggc tgatctccgc cctggctgct cgaccaggcg gcaccgaacc
     1981 acagctcgac cagcgcgtca cgcaccgcgg cctggccttc tgcttccagc ttaccctcca
     2041 gcaagcgctg cttcatcagg gcgttcgagc gcagatgctc cctttgtcga tcggacttgg
     2101 acgaaaaccc ctgacccgat gctcgctgca tgtacaggcc atcggtcacg agaccgcatc
     2161 tcgcctgcag cgcggcacgt atcgcccagt cgcagtccca gagcaggata tctgcctggt
     2221 acggaccgat ccgctcgaaa ctcgaacgtc tgacgaccgg gcgctgaaac gccatcggca
     2281 cagtcctcag cagtccgcgg aacaactccg ccggctcgaa cgcgaggact ccccccctgg
     2341 gagaggagaa cggatggagg gtcgacagca gcgcccccat cgcctgttcg taggccctct
     2401 ggcccgcctc gctccgctcg ccgaaccatg agacgcccat gaacaccaca tcgagctcgg
     2461 ggtcctccgc gagggccgcg actgcacgct gcagatagga cggagcgtag agatcgtcgt
     2521 catcgaggaa tgccagcacg tcgccccggg cctggaaggc gcctgcattc ttcgcctcgg
     2581 caccaccgcg gctgcgaacg ttgcggatga gcgcgcaacg ggggtcatcc ggtatcgacg
     2641 cgggcggatc cgaggcgtcg tcgacgacga tcagatccca gttctcccag gtctgagcgc
     2701 ggacacttgc gattgcttcg gccaatagtc gaggccgatc atgagtcggg aggataacgg
     2761 tgaccgacgg tgaacgtgtc gaggtgctca tgagcccccc tcggtgcgcc ggagcctcga
     2821 tctcatcggt gcgacctgga gctcgcgaat cggccaccgg caacgcgggg ttgcgggaga
     2881 tggctcttgt cgggcggcgt cgggccggca ctcgacaatg gccgctcgag cccggaggcg
     2941 ctgcttcccc gagcttgcac ccgcggttcg gcaatttccc cgaccgcccg ccgaaccagt
     3001 tcgcgcacta ccacgatgat cacgatcagg tggtaaaaca ggtcgaaata ctgcaggccc
     3061 aggaaggagc cggccgacat gtagccgatc aggctgacct ggatcatcgc ggcgaggtcc
     3121 cgcgcccagg tcagttcctt gtgcggcttc accatgcgga tgatctgtgt cgccgaccgc
     3181 caggtgaaga tgtgcagcag cacgaacatg acgagaccgg gccagccctg ctcgccgagc
     3241 gtaccgaacc agttgctgtg cgaatcggtg cctccgaagc ccgtgaccgc gcggaaaccc
     3301 ccgccgagca ggctggagtt ggcgatgtag acggcattcc cccaggcgta gatgcgccct
     3361 tgtgccgagc cgtcctcatc ataggtctgg atcgtgtgca tgcgctcgaa ccactccggc
     3421 ggcatcacgt agaacacgac cagcaccacc gggatgatcc cgatcaggac cccgagccgg
     3481 ttgcgcgcct tgagcaggaa gaacaccccc atggcgccga gcccgaccag cgcaccgcgc
     3541 gaatgcgtgc cgatcacggc gatcagggtg aggacggaca tggcgagcag ccccaggcgc
     3601 tgccaggcca ccttgagctg caggcggatg taccagagca gcgggaccgt catcagcagg
     3661 gcgaggccga tcgagttgcg gtcctcgatg aaggagccgc cggggccgcg cacgttgttt
     3721 ccgctgcccc cggtcaggac gaagatgccg cccttcaccc cgtagaaggc cagcgagaag
     3781 gcgatcacga ccaccagcca gtgcacgcgg tcgcggtcgc ggatcagcat cagggtgacg
     3841 taggtcatca acatgatccg ccacaccttg tcccactgcg gccaggccag gcccggatac
     3901 cacgagaagg ccgtggtcag gaacatccat ccggtgaaca ccagcatcag ccaggtctcg
     3961 cgcgaccaca cgatctcctt cttctcgcgc gagaacagga tgccgacgaa agtggcgagc
     4021 gcgaccatga atgcgaaggg catcgaggtc gagaagcccc aggccatccg gtgcggattc
     4081 atgtagctca gccacgacca cgcgaggatg ccgatccacg ggtatctgag gatgaagggc
     4141 agcgtgccga acaccgcgag ggtgacgatc aggtcgcgca tgacacctcc gaggcgtcga
     4201 ccttgctgac gacgtagcga tactttccgg acttctccgg cgcaatctcg gcaacctgct
     4261 ccacgtcgat ccgcaccccc tcgccgagcc gggccgccag accgtgctcg atccgaccga
     4321 cagtgtcggc gtcgaacccg gcgcccggca ccacctgcac ccgagtgtgc tccagcgact
     4381 cctgcacgat gcggaagccc gccacctggg gcaggtcgcg cagcacgtag atcagcgcca
     4441 gcccgtgcat cacggtgccg tcgcgggcga cgacgaagtc ggtcgcgcgt ccctggatct
     4501 ccctcagcat cggcaggccg cgcccgcagg cgcaggtcgc ggcgtccatc accgccacgt
     4561 ccccggtgcg atagcggatg aagggaaagt cgccggtggc gagatgggtg acgacgatct
     4621 cgcccgcctg cccgggcggc agcaccctgc cctcgccatc gacgatctcg acaatgaggt
     4681 cctcggcagt gaggtgcatg ccgccttgcg ggcactggtg ggcgatgaag ccggcgtcgc
     4741 gcgagccgta gccgttggcg accgggcagc cgaagacacg ctcgatggcc tcgcgctggt
     4801 ggtcgtagag gcgctcggag gtgcagaacg ccacctcgat gccgaggtcg tccatgcgaa
     4861 tcccgcgcct ttcggcgtgc aggccgatat gggacagcgc cgaagggtag ccgaagagca
     4921 tcttcgggcg gcgcgcgcgg atgcgggcga cgaagccgtc cagcttcgtg gcggacatct
     4981 cgaaggcggg cagcagctcg gtacggaaca gccgatcccg cagtgcacgc acccggtcct
     5041 gcgcgccgag ctcgatcggc gagccccaca cgacaatctc gggatcgccg atgtcgacgc
     5101 cccaccagcg cgtcgcgcgc cacttggcgg cgacgtcgtg gctcacacgc cggtcgccga
     5161 tgaagaagat gagcggctcg ccgctcgagc cgccggtgtt gaagcgcgcc agtccgaccg
     5221 cgtcgtcggc cttcaaggct tcggtattgg cgcggatctc cgccttgccc aggaagggca
     5281 ggcgctgcag ctcggccagg ctggcgatcg ccgcggggtc gaagccgatg cgcgcgaagc
     5341 cttcgcggta atagggcacg tggtcgcgcg catgcaccag cagcgcacgc aggcgcttga
     5401 gctgcagggc ctcgatgcgc tcgcgcggcc accactggct atcctccagg ccgcgccgca
     5461 gggcgacgct gtcgtggtgc ttgaggcgct cgtggagcgg gaaaagcagg cccgagacca
     5521 ggcgggtgta gaggccggac atttcgtttt cctccgcctc aagggttcgc ggtgcgatcc
     5581 agcatggcct gcaggggccc gcccatctcg cgtgcgaagt cttcaagcgc ggcgatcgtg
     5641 ccggcgtgtc cgccctgacc ctgcggcgca tgcagcatga ccaccgccga atcgtcccct
     5701 tcgccgcgca ggcggctcag cgccagatag accttggcaa gatgatcgct ggtggtccac
     5761 cggccgccga tccagtacgc gtaccagacg accatgcgtg catgctcgct cgccatctcc
     5821 agcgtgcgcc acgcctgcgc ctggtcgccg agttgcaccc ggaccggacc cgcggcggtc
     5881 atgcgccaca ccgggtcctt gctgatcagc acgcggttct cggaattgat caactccctg
     5941 cccggtccct ggtcgcggta atagccgatg tacaccccca ccggaacgct ccccgcgcgc
     6001 caggtcgaac ggaaggtgtc gcgcatgccg gaatagctcg gcgaccacgg cggcaattcg
     6061 cccgcttccg tccgctgcca cgcgccctgc ccctccagcg cggcgaagcg caccggcccc
     6121 tgctcgccct ggcgctcgag gtggccgagg accggcaccc acagccccac cgcgaccacc
     6181 gccacggcca gccagcccag gccaccggtg cgtgcgcccg acgatcgcgc ggagccgacg
     6241 ggcatggccg gggcgtcttc ctcgtcctcc tgccagcgcg aaccgatcca gaacagcgcg
     6301 aggatgatga tgccgaagaa gacccagccg tagatcaggt ggtcggcccc ggcggcgagg
     6361 cggttgtcgg tgaggtggcc gagcatcacg atgccgtagg cgcgcagcca gttggcgaat
     6421 accggcagga cgatcgaggc ggcgacgaaa agcaggcgct tctgcgtgct gcggtagttc
     6481 aggtaagcgt acaggctgcc gacgacgatc gaggccatca ggtagcgcac gccgctgcaa
     6541 ccctccacca cctgccagcg cccggagggg atcaccagca ggaagccctc ggcgtacacc
     6601 ggcacgccgg aggcgcgcac cgcccagatg atgaagtcgg cggtccactg catcatggtg
     6661 gggaagagga actcgccgaa cggcaccatg aagaacagga agccgatcgg gaaggcgtag
     6721 gtgcgcacca ccgcgctgcc catcaccgcc cagacgaagc ccaccagcat gcccacgaag
     6781 gcgaactgcg acaccgcatc gacgctggcg agctcgccga gcagccaggc cgcgccggcg
     6841 acgagtccgg cgcagatgcc gagcagcgag ggcgcgatcg gtacatggtc gatgaagtgc
     6901 cggcgccgcc ataccagcca ggccgagatc ggcgcgatca cgaagccgtg ggcgaaggtg
     6961 tccgagcggt cccagatgcc caccatggcc aggaaggtgt cgcgatacca gaacagcagc
     7021 cacagcagtc ccagcgcaag caccgccagg tgaagcctgc gcgtgcgcgg atcaccgagc
     7081 gtcgcagggc gggcgtcggt cgcctgcagg tgggtcggca tcgcgcccgg ccgcacggat
     7141 tgaagcatcg cggttccttt catgttcatg tggccctctt cgcggcaatc gcatccgcgt
     7201 aggcggacag cagttgtgcg cgcaccgagc tccagcggta gcgctgcacc tcggcgcacc
     7261 cggcggcgac cagcgcggcg cgcaggccgg tgtcggcaag caccttgtgc gcggcgtcgg
     7321 ccatcgcctg cggatcgtcg ggtggaacca gcatggccgt gtggccttgc gtgacgatga
     7381 agggaatgcc gccgacgtcg gtggtgacca ccgccaggcc gctcgccagc gcctcgagga
     7441 tggcattggg catgttatcc acccgactcg ggttgagcat gagatctgcg tcacggtaga
     7501 gcgcagccat ctgctcgcgg tccaggcgac cggtgaagcg gacccgctcg gcaatgccga
     7561 gttcctgcgc gagcgcggcc agcgccgcgg cctccggccc gctgccggcg atcgagaggc
     7621 gcgccg
//
LOCUS       DAOQNN010000131         7618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5006_length_7618_cov_8.197805, whole genome shotgun sequence.
ACCESSION   DAOQNN010000131 DAOQNN010000000
VERSION     DAOQNN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5006_length_7618_cov_8.197805"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PKV97_08550"
     CDS             complement(<1..978)
                     /locus_tag="PKV97_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HNB05963.1"
                     /translation="MNTPRTVSPAAAVRTLAIAGMNCASCVAHVEKALLKVEGVQAAS
                     VNLATETATVTAPAAVPLSALIAAVEKAGYAVRRDTLELDIEGMSCAACVGRVEKVLQ
                     RVPGVVTANVNLASRRAHVELAGGVPTAALVEAVQKAGFGARPAAEVGEGAREADAAA
                     EEAALRRAVWIALALTLPVFVLEMGSHLIPGMHDWVMATIGHRESWLLQFVLTSAVMF
                     GPGLRFFRKGVPALLRGAPDMNALVAMGTSAAWGYSVVALFAPALLPAGTVNVYFEAA
                     AVIVTLILIGRLLEARARGRTSDAIRRLLRIQPRSARVRRGAEGVEGVEI"
     gene            1271..2734
                     /locus_tag="PKV97_08555"
     CDS             1271..2734
                     /locus_tag="PKV97_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNB05964.1"
                     /translation="MSTSNSIRSGIKWLVTGSLGGQVLQFAFGIVLARLLVPEDFGFI
                     VTIQIFTGFVGLISGGGMGQALVQSRDAQARDFDVVFTAQLLIGLLIFAGFYVAAPWF
                     AHTFGDALYADLVRVSAISFLLRPFVNTRASALHRDMRFKERSVIGLAVAVLTGLASI
                     ALAVVGAGPWSLILSGILGSLITLAMLDRVSDRRPQLCLDLEVARRFGAYGVKVSLND
                     LAAYFTKQASNAIISRVAGPSAVGLFNKGESLAWLPFSTVSAAVYDAVFRAMAKAQDR
                     PDEVKYLFYRMIGLMVVYTLPIYIGLAWMAEPFMLFVYGAKWVPSADPLRIVSLAGLL
                     ICIGHPCGAVLAAMNRLGREVWIHIAQGVLVSVACYYGLKHSGISGAAWGVFAGIAFS
                     TPVMYYLATRCFPSRLGDLWRALAPGLGLNLILLAVLALADAALPADWREIRPALYML
                     VTGGSAAIAYAAAFLFLPLATLADESLRWRRTLRLAR"
     gene            2741..4726
                     /locus_tag="PKV97_08560"
     CDS             2741..4726
                     /locus_tag="PKV97_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05965.1"
                     /translation="MKHLDLLEHFSAADGGIAMQLRGAQRRLGSHDGVDIVRDDFADE
                     AASALFLRVQRTATAESVRLRLAGNHLLKRCAIGGWMFEPTTPGEAVFWVPRLPVCRT
                     LDGVGRIRAESPATLANMEIGGGEVAATFELAPDQVLDCVVWRLESEASGTDGSRAAN
                     ELMTASALESQAFFAYASHTATRCAADFYTHIVHGQVYGACWAWPKKLKICDELDALA
                     LHMVASALGHSTGKRVYRLLRRQIVLAVLCRQDADGGFRHGEWTDEYESHNRLINGAM
                     QLLATEFAAAPEPALEGALRRIARYLAEQVDHTDAGAWFLHDSLERSEEGMRHYPLAW
                     ERGRWLGKSPTNLLILNTHLDCMLALARERAACGDDTHDGLLRSAEACLRTVMSARPA
                     DWLFRPLLAVIALSLLPKAEQEQLPFARRALKRLGWKYLIPRWHLIRRRFPRLLMPAG
                     YIERSLGQADFVHRYHGVHLMDFERLLACGAVDPKDPRWTSISDGLVDFGVNSRVTRL
                     WKETAASRDSLAFWAEGLYRRCLRTRAQRDRELLATAVLDLHDAGLGLPPSLLGANGE
                     IVPAQSAAPTPSAADARLRVINLGARKTPCLLVVNTATTDLPLAFEPPLTAAHPGWMD
                     ATRSVPARGWILLQPGPSDAEPGPASSSHAFLPARPR"
     gene            4729..5805
                     /locus_tag="PKV97_08565"
     CDS             4729..5805
                     /locus_tag="PKV97_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNB05966.1"
                     /translation="MSNARAIAFHLPQYHPTPENDEWWGKGFTEWTNTTRAKPLYPGH
                     YQPHLPADLGYYDLRLPEARHAQAELARAYGIEGFCYYHYWFGGRRILERPVNEILAS
                     GEPDLPFCLCWANHSWSNIWQGVADRMLIEQTYPGMDDHRRHFEWLLAAFHDPRYIRV
                     DGKPMFLIYNPPDIPDVARVMDYWRELAAQAGLPGLHLVAVNYLGAAVDPADFGMDAA
                     TWQPLPPKSGHIPWRYPALKARMRMAKGKYKLTVLDYARIMSGLTRGSPPQFTEYPTV
                     LPNWDNTPRSGLNGLVLHGSTPELFKTVLRRGVDLVQGYPAEQRIVFIKAWNEWAEGN
                     YLEPDQRFGHGYLRAVREVLQETR"
     gene            5807..>7618
                     /gene="asnB"
                     /locus_tag="PKV97_08570"
     CDS             5807..>7618
                     /gene="asnB"
                     /locus_tag="PKV97_08570"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNB05967.1"
                     /translation="MCGINGIFAYAPAANAVDRAELLRVRDTMIERGPDGAGVWIGDD
                     GRIGLGHRRLAILDLSAAGAQPMHSADGTLVVSFNGEIYNYPELRGDLERAGVEFRSH
                     SDTEVLLHLYRRHGPAMVGRLRGMFAFALWDARARSLLLARDPHGIKPLYYADDGRTL
                     RFASQVSALLAGEGVDTRPEPAGAVGFLLWGSVPEPFTLYRGIRLLPAGSTLLVNAAA
                     GAGAAVRYWSLSAALQRSVQAAAAVPAGGEAAFMRERLLESVRAHLLSDVPVGAFLSA
                     GLDSSTLVGLAREASPARLRTLTLTFDDLQGTAHDECPAASLIARHLDVDHTCIVLSA
                     GEQEDELDRFFAAMDQPTIDGINTWFVSRAAQQAGLKVALSGLGGDELLGGYATFAEH
                     PRLRARAHASPWRHAPRLYRGAHRLLGARLGWPPNAAGIPACGADPEGAYHLLKGVFM
                     PWELDGVVDADVLCEGLQALAEHDAAHTEALAGLNDFGQVACLESVRYMRNQLLRDTD
                     WVSMSHSLEIRVPLVDHLLSEALLGLAASGRLGPGKSMLPRTLARGLPDEILNRPKSG
                     FVVPTWRWLRHHPGLDAWKSLPALRHPRMSDGRRWAYT"
BASE COUNT         1100 a         2870 c         2476 g         1172 t
ORIGIN      
        1 gatctcgacc ccctccaccc cctcggcacc gcgccgcacg cgcgcgctgc gcggctggat
       61 gcgcagcagg cggcggatgg cgtcgctggt gcggccgcgc gcgcgcgcct cgagcaggcg
      121 cccgatcagg atcagcgtga cgatcaccgc cgcggcctcg aaatacacat tgaccgtgcc
      181 cgccggcagc agcgcgggcg cgaacaaggc caccaccgaa tagccccagg ccgccgacgt
      241 gcccatcgcc accagcgcgt tcatgtccgg cgcgccacgc agcagcgcgg gcacgccctt
      301 gcggaagaag cgcagcccgg gcccgaacat caccgcgctg gtcagtacga actgcagcag
      361 ccagctctcg cggtggccga tcgtggccat gacccagtcg tgcatgccgg ggatcaggtg
      421 cgaacccatc tcgagcacga acaccggcag ggtgagcgcg agcgcgatcc acaccgctcg
      481 tcgcagcgcc gcctcctcgg ccgcggcgtc ggcctcgcgc gccccctcgc ccacctccgc
      541 cgccggacgc gcgccgaagc ccgccttctg cactgcctcc accagggccg cggtcggcac
      601 gccgccggcg agctcgacgt gggcgcggcg cgaggccagg ttcacattgg ccgtcaccac
      661 cccgggcacg cgctgcagca ccttctcgac gcggcccacg caggccgcgc agctcatgcc
      721 ctcgatgtcg agctcgaggg tgtcgcgccg caccgcgtag ccggccttct cgacagcggc
      781 gatcagcgcc gacagcggca ccgccgccgg agccgtcacg gtggcggtct cggtggccag
      841 gttgacgctg gcggcctgca cgccctcgac cttcagcaag gccttctcga cgtgggcgac
      901 acaggaggcg cagttcatgc cggcgatggc gagggtgcgc accgcagcgg cgggcgagac
      961 agtccggggg gtgttcatgg tgtccatcca gtacggaacc gggatagtcc gcactgtaga
     1021 ccttgccaac gtgggaaggt caacccgacc tccgcggtcg ccacgccaag cgggcccgtg
     1081 cccgtgaaag cctgcgccac ctcggccggc gccgcaggtg cggagcccga gcgaagtcgc
     1141 aatcgacccc gcgcgcccct cttcgcatgc gtcccggcgc gtgtagaggc cataattcac
     1201 ggcttgttca cgccttcccg ggcacaatgc ggggcaacat tccgcccgct taggcctcgt
     1261 tcccatccag atgagtacat cgaactcgat tcgcagcggc atcaagtggc tcgtcaccgg
     1321 cagcctcggc ggtcaggtgc ttcagttcgc cttcggcatc gtgctcgcgc gcctgctcgt
     1381 cccggaggat ttcggcttca tcgtcaccat ccagatcttc acgggtttcg tcggcctgat
     1441 cagcggcggc ggcatggggc aggcgctggt gcagtccaga gacgcgcaag cgcgcgattt
     1501 cgacgtggtg ttcacggcgc agttgctgat cggcctcctg atcttcgccg gcttctacgt
     1561 cgctgcgccc tggttcgcgc acaccttcgg cgatgcgctc tacgccgacc tcgtccgcgt
     1621 ctcggcgatc agcttcctcc tgcgcccctt cgtgaacacg cgtgcgagtg cgctgcaccg
     1681 cgacatgcgc ttcaaggagc gctccgtgat cgggctcgcc gtcgcggtcc tcaccggcct
     1741 cgccagcatc gcgctcgcgg tggtgggcgc cggcccgtgg agcctgatcc tgtcgggcat
     1801 cctgggcagc ctgatcaccc tcgccatgct cgaccgcgtg agcgaccgcc gcccgcagct
     1861 gtgcctggac ctcgaggtgg cacgacgctt cggcgcctac ggggtcaagg tttcgctcaa
     1921 cgacctcgcc gcctatttca ccaagcaggc cagcaacgca atcatcagcc gggttgcggg
     1981 gccctcggcg gtcggtctct tcaacaaggg cgagagcctt gcctggctgc ccttctcgac
     2041 cgtgagcgct gcggtctacg acgcggtctt ccgcgcgatg gcgaaggcgc aggaccgccc
     2101 cgacgaggtc aagtatctct tctatcgcat gatcggcctg atggtcgtct acacgctgcc
     2161 gatctatatc ggcctggcgt ggatggcgga gcccttcatg ctcttcgtct atggcgcgaa
     2221 atgggttccg tccgccgatc cgctgcgcat cgtctcgctc gccggcctgc tgatctgcat
     2281 cggccacccc tgcggcgcgg tactcgcggc gatgaaccgc ctcggccgcg aagtgtggat
     2341 tcacatcgcg cagggcgtgc tggtatccgt cgcgtgctac tacggcctga agcactcggg
     2401 catcagcgga gccgcatggg gcgtcttcgc cggcatcgcc ttctccaccc cggtgatgta
     2461 ctacctcgcg acgcgctgct tcccgagccg tctcggcgac ctgtggcgcg cgctcgcgcc
     2521 cggcctcggc ctcaacctca tcctgctggc ggtgctggca ctcgccgatg cggcactccc
     2581 ggcggactgg cgtgaaatcc gtcccgccct gtacatgctg gtcacgggcg gaagcgccgc
     2641 gatcgcctac gccgccgcct tccttttcct tccgctcgcc acactcgcgg acgagagcct
     2701 gcgctggcgc cgtacgctcc ggctcgcccg ctgacgaacc atgaagcacc tcgacctcct
     2761 ggagcacttt tcggccgccg acggcggcat cgccatgcag ttgcggggcg cgcagcggcg
     2821 cctcgggtcg catgacggcg tcgacatcgt gcgcgacgat ttcgccgacg aggccgcgag
     2881 cgcgctcttc ctgcgcgtcc agcggaccgc caccgcggaa tcggtccgcc tgcggctcgc
     2941 cggcaaccac ttgttgaaac gctgcgccat cggcggctgg atgttcgagc cgacgacacc
     3001 tggcgaggcc gtcttctggg tgccgcgcct gccggtatgc cggaccctgg atggcgtcgg
     3061 ccgcatccgc gcggaatccc cggcgacgct ggcgaacatg gagatcggcg gcggcgaggt
     3121 ggcggccacg ttcgagctcg cacccgacca ggtgctcgat tgtgtcgtct ggcggctgga
     3181 gtccgaggcg tcgggcacgg acgggagccg cgccgcaaac gagctgatga cggcaagcgc
     3241 actcgagtcc caagctttct tcgcatacgc ctcccatacc gcgacgcgat gcgcggcgga
     3301 tttctacacg cacatcgtgc acggccaggt ctacggcgcc tgctgggcct ggcccaagaa
     3361 gctgaagatc tgcgacgagc tcgacgcgct cgccctgcac atggtcgcca gcgcgctcgg
     3421 ccacagcacc ggcaagcgcg tctaccgcct gttgcgccgc cagatcgtgc tggccgtcct
     3481 ctgccgccag gacgccgacg gcggtttccg gcacggcgaa tggaccgacg agtacgagag
     3541 ccacaaccgc ctcatcaatg gcgcgatgca gttgctggcc accgagttcg ccgccgcgcc
     3601 cgagccggcc ctggagggcg cgcttcgccg gatcgcgcgc tatctcgccg agcaggtcga
     3661 ccacaccgat gccggggcct ggttcctgca cgattcgctg gagcgctccg aggaaggcat
     3721 gcgccactac ccgctggcgt gggaacgcgg acgctggctg ggcaagtcgc cgacgaacct
     3781 cctcatcctc aacacccacc tcgactgcat gctggcactc gcccgagagc gcgcggcgtg
     3841 cggcgacgac acccacgacg gactgctgcg ctccgccgag gcctgcctgc gcacggtgat
     3901 gtccgcgcgt ccggccgact ggctgttccg cccgctgctc gcggtgatcg ccctctcctt
     3961 gctgcctaag gcggagcagg aacaactgcc gttcgcgcgc cgcgccttga agcggctcgg
     4021 ctggaaatac ctgattccgc gctggcacct gatccgccga cgctttccac gcctgctgat
     4081 gcccgccggc tacatcgaac gcagcctcgg ccaagccgat ttcgtgcacc gctaccacgg
     4141 ggtgcacctg atggatttcg aacgcctgct cgcgtgcggc gccgtcgacc cgaaagaccc
     4201 tcggtggacg tccatcagcg acggcctggt cgacttcggc gtaaatagcc gcgtcacccg
     4261 actctggaag gagaccgcgg ccagccggga ctcgctcgca ttctgggccg agggcttgta
     4321 ccgccgctgc ctgcgcacgc gcgcgcagcg cgaccgcgag ctgctggcga ccgcggtgct
     4381 cgacctgcac gatgccggtc tcggcctgcc gccctcgctg ctcggcgcca acggcgagat
     4441 cgtgcccgca caatcggccg cgcccacgcc gtctgccgcg gatgcacgcc tcagggtgat
     4501 caacctgggc gcgcgcaaga ccccctgcct cctcgtggtc aacaccgcca cgacggacct
     4561 gccgcttgcc ttcgagccgc cgctcacagc cgcccacccc gggtggatgg acgctacgcg
     4621 gagcgtgccg gcgcgcggct ggatcctgtt gcagcccggc ccgtccgacg cagagcccgg
     4681 cccggcctcc tcctctcacg cattcctgcc tgcacggccc cggtaacgat gagcaacgcc
     4741 cgcgcgatcg ccttccacct gccccaatac caccccaccc ccgagaacga cgagtggtgg
     4801 ggcaagggct tcaccgagtg gaccaacacc acccgggcga agcccctcta cccgggccac
     4861 tatcagccgc acctgccggc cgacctgggc tactacgacc tgcgcctgcc cgaggcccgc
     4921 cacgcgcagg ccgagcttgc ccgcgcatac ggcatcgagg gcttctgcta ctaccactac
     4981 tggttcggcg gacggcgcat cctcgagcgc ccggtcaacg agatcctcgc cagcggcgag
     5041 cccgacctgc cgttctgcct gtgctgggcc aaccacagct ggagcaacat ctggcagggc
     5101 gtggccgatc gcatgctgat cgagcagacc tacccgggca tggacgacca ccgccgccac
     5161 ttcgaatggc tgctcgccgc attccacgat ccgcgctaca tccgcgtcga cggcaagccg
     5221 atgttcctga tctacaaccc gcccgacatt cccgacgtgg cgcgggtgat ggactactgg
     5281 cgcgagctcg ctgcgcaggc ggggctgccg gggctgcacc tggtcgcggt gaattacctc
     5341 ggcgctgcgg tcgatccggc cgacttcggc atggacgccg ccacctggca gccgctgccg
     5401 cccaagagcg gccacatccc gtggcgctac ccggccttga aggcgcgcat gcgcatggcg
     5461 aagggaaaat acaagctgac cgtgctggac tacgcccgca tcatgagcgg tctgacccgc
     5521 ggcagcccgc cgcaattcac cgagtacccc acggtgctcc cgaactggga caacaccccg
     5581 cgctcgggtc tcaacggact ggttctgcac ggctcgaccc cggagctctt caagaccgtg
     5641 ctgcgccgcg gcgtcgacct ggtgcagggc tacccggccg agcagcgcat cgtcttcatc
     5701 aaggcctgga acgagtgggc cgagggcaac tacctagaac ccgaccagcg cttcggccac
     5761 ggctacctgc gcgcggtgcg cgaggtcctg caggagacgc gctgaagtgt gcggcatcaa
     5821 cgggatcttc gcctacgccc ccgctgcgaa cgcagtggac cgcgccgaac tgctgcgggt
     5881 gcgcgacacc atgatcgagc gcggccccga cggtgcgggc gtgtggatcg gcgacgacgg
     5941 gcggatcggc ctcggccacc ggcgcctcgc catcctcgat ctcagcgcgg cgggcgccca
     6001 gccgatgcac tcggcggacg gcacgctggt ggtcagcttc aacggcgaga tctacaacta
     6061 ccccgagctg cgcggcgatc tcgagcgcgc cggcgtcgag ttccgctccc attccgacac
     6121 cgaggtgctg ctgcacctgt atcgccgtca cggtcccgcg atggtgggcc gcctgcgcgg
     6181 catgttcgcc ttcgcgctgt gggacgcgcg ggcgcgcagc ctgctgctcg cgcgcgaccc
     6241 ccacggcatc aagccgctgt attacgcgga cgacggcagg accctgcgct tcgcctccca
     6301 ggtctccgcc ctgctggccg gcgaaggcgt cgacacgcgc cccgaacccg cgggagcggt
     6361 cggattcctg ctgtggggct cggttccgga accgttcacg ctctaccgcg gcatccgcct
     6421 gctgccggca ggctcgaccc tgttggtgaa cgcagcggcc ggcgccggcg cagcggtacg
     6481 ctactggagc ctgtccgccg ccctgcaacg ctcggtgcag gccgcggcgg ccgtcccggc
     6541 cggcggggag gccgccttca tgcgcgaacg cctgctcgag agcgtgcgcg cccacctgct
     6601 ctccgacgtt ccggtcggtg cgttcctgtc cgcagggctg gattcgtcca ccctggtcgg
     6661 cctcgcccgc gaagcctcgc ccgcccgcct gcgcacgctc accctgacgt tcgacgacct
     6721 gcagggcacg gcgcacgacg aatgtccggc cgcgagcctg atcgcccgcc atctggacgt
     6781 cgaccacacc tgcatcgtgc tctctgcggg cgagcaggag gacgagctcg atcgcttctt
     6841 cgccgcgatg gaccaaccga ccatcgacgg catcaacacc tggttcgtga gccgcgcagc
     6901 acaacaggcc ggcctcaagg tcgcgctctc gggcctggga ggggacgaat tgctcggcgg
     6961 ctacgccacc ttcgcagagc atccgcggct gcgcgcgcgc gcccacgcca gcccctggcg
     7021 gcacgcaccc cggctctacc gcggcgccca ccgcctgctc ggtgcccgcc tcggctggcc
     7081 gcccaacgcg gccgggatcc cggcttgcgg cgccgatccg gagggcgcgt accatctgct
     7141 caagggcgtg ttcatgccct gggaactgga cggcgtggtc gatgccgacg tcctctgcga
     7201 aggcctgcaa gccctcgccg aacacgatgc ggctcacacc gaggcgcttg cggggctgaa
     7261 cgacttcggc caggtcgcct gcctggagtc ggtgcgctac atgcgcaacc agctgctgcg
     7321 cgacaccgac tgggtcagca tgtcgcactc gctggagatc cgggtgccgc tggtggacca
     7381 cctgctcagc gaggccctgc tcggtctcgc cgcgagcgga cgcctcggcc cgggcaagag
     7441 catgctgccg cggacgctgg cacgcggcct gcccgacgag atcctgaacc gccccaagag
     7501 tggcttcgtc gttcccacct ggcgctggct gcgccaccat ccggggctcg atgcgtggaa
     7561 gtccttgccc gcgctgcgcc acccgcgcat gtccgatggc cggcgctggg cctacacg
//
LOCUS       DAOQNN010000132         7510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5125_length_7510_cov_9.213146, whole genome shotgun sequence.
ACCESSION   DAOQNN010000132 DAOQNN010000000
VERSION     DAOQNN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5125_length_7510_cov_9.213146"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PKV97_08575"
     CDS             <1..1236
                     /locus_tag="PKV97_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB05968.1"
                     /translation="LARNIWPDRHFPGCRFAERCPAAFDRCDEEVPVWHAVGTQAVRC
                     HLYEGVAIPRRFVRETAGAADVVAARPAQSVLEVAELAVHFPVRKGFLRRTVGHVKAV
                     DGVSLRLQPGRTLALVGESGCGKTTVGKALLRLIEPTTGRLWLDGERYDALPLSALQP
                     LRRAVQMVFQDPFASLDPRMRVGEIIEEGLAALGVGADHRARQARVTELLERVGLSAE
                     MRWRYPHEFSGGQRQRIAVARALAVAPRVIVCDEPTSALDVSVQAQLLNLMRELQHEQ
                     GLAYLFITHNLAVVEYLADEVAVMYLGRIVEEGPIERVLGVRAHPYTRALLAAVPRIE
                     GAGGAAPDVGGTGVAGGSTTTPGTIMAELPSPLAPPSGCHFHPRCPYATDVCRIRYPA
                     PADLGQGHRVRCHWPLALR"
     gene            complement(1394..2770)
                     /locus_tag="PKV97_08580"
     CDS             complement(1394..2770)
                     /locus_tag="PKV97_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNB05969.1"
                     /translation="MTSSASCAAQPRGGRRHGPLLAVCACALALAALASPASARDVTA
                     APATLTASDTAASAAVATSAPETARVLTLDLTRDANDIWDRIRRGFGMPDLDSQRVMD
                     QQSFYLNRPRFLKQVFERGGRYLYYIVDELERRGMPTELALLPMVESSYNPMAYSRAH
                     ASGLWQFIPSTGKHYNLTQDHWVDERRDVIASTNAALDYLQRIYEMHGDWHLALASYN
                     WGEGAVQRALKRNAEDGLPLEYEHLRMPDETRNYVPKLQAIKNIVAEPELFHFELPYV
                     ANELHFVSVEAPIGVDLATAAKLSGMPLDEFLALNPGFNRPAVTTPGQIFIVPTDRAE
                     QLQQGLAELDEEDGEWRMHVMAAGETLEAVAARHGLSSAELRRLNGLGPGSQPGEGHS
                     LLVPDGIDPDGALEVTSLLPFAATAARQPGLKLNTSQARSGEPHLKSPKARPRPGEKR
                     STKRSKKR"
     gene            complement(2777..3550)
                     /gene="gloB"
                     /locus_tag="PKV97_08585"
     CDS             complement(2777..3550)
                     /gene="gloB"
                     /locus_tag="PKV97_08585"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HNB05970.1"
                     /translation="MDIIPVPAFRDNYIWLVHDGRHALVVDPGDATPVEEALREHGLV
                     LGAILVTHHHPDHTGGIAALVASHRVPVYGPARESIAGVVHAVSDGDVIHLPAPAIAL
                     RVLDVPGHTAGHVAYVGMDIEPGLVFCGDTLFSAGCGRLFEGTPTQLAHSLARLAALP
                     PDTRVFCTHEYTLSNLAFARAAEPENVERDAWAAHCETLRARGEPTLPSTVSRELAIN
                     PFLRCDQPGVIEAVAAHTGSRPQDALECLAALRAWKDRF"
     gene            3579..4304
                     /locus_tag="PKV97_08590"
     CDS             3579..4304
                     /locus_tag="PKV97_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB05971.1"
                     /translation="MSILRLEEWLQTPQGRYLLDWEQDGLDRLVADIFGYHAVQLGLS
                     GFDLLRANRMPRRIDCGPQGRDLRCSEYELPFATGSLDLVLLPHVLEFSAHPHRVLRE
                     VERVLVPEGSVVISGFNPLSLWGLRRLIARRGAEAPWSGHYRSAMRIRDWLNLLGFEI
                     QESRFGCYAPPLRTPQWLERWGCLDRAGSRWWPVLGAGYLLHGVKRVQGMRLITPHWR
                     GRKAAAKRLSPVAQRDGSTRNMQ"
     gene            4307..4765
                     /gene="rnhA"
                     /locus_tag="PKV97_08595"
     CDS             4307..4765
                     /gene="rnhA"
                     /locus_tag="PKV97_08595"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNB05972.1"
                     /translation="MEEVDIYTDGACSGNPGPGGWGAILRSGSHEKEIWGGEPATTNN
                     RMELLAVIRALDALKRPVAARVHTDSQYVQKGISEWIHGWKARGWKTASKEPVKNADL
                     WRALDDAASRHQVKWLWVRGHNGHPENERADALARRGVDAVRKSGAAVQC"
     gene            4779..5498
                     /gene="dnaQ"
                     /locus_tag="PKV97_08600"
     CDS             4779..5498
                     /gene="dnaQ"
                     /locus_tag="PKV97_08600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HNB05973.1"
                     /translation="MRQIVLDTETTGLDWRNGDRVIEIGCVELLDRSLTGRHYHVYIN
                     PGRGIDAEAVAVHGITEEFLADKPKFADIVADFEDFVRDAELVIHNASFDVGFLDAEL
                     ERLGRAKLGALCAGVVDTLKLAKEQNPGKKASLDALCDRYEIDNAHRELHGALLDAEL
                     LAEVFLAMTRGQESLMIGLDEEAAPRESDSEGAPVLRAAVRVLRASADECAAHDAALA
                     DIAKANKGLCLWLPPEPASAG"
     gene            complement(5699..6106)
                     /locus_tag="PKV97_08605"
     CDS             complement(5699..6106)
                     /locus_tag="PKV97_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB05974.1"
                     /translation="MLGSLLVKDYMVGDHLAFSPDTDLLKAVRLLLQRGLSGAPVVDE
                     NRRLIGFLSEKDCLKAALDASYFRRDEGKVEDFMNRDVVSIRASASLIDAIELFLARN
                     HHILPVVEGARLIGQISRHDILKGLEKVRRDER"
     gene            complement(6122..6325)
                     /gene="nqrM"
                     /locus_tag="PKV97_08610"
     CDS             complement(6122..6325)
                     /gene="nqrM"
                     /locus_tag="PKV97_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HNB05975.1"
                     /translation="MNTFLITLLVVGLVILAMAVGVLFGRKPISGSCGGLGSMGQDCE
                     FGCKKPCSKRQARMMAAQAEQPH"
     gene            complement(6456..7226)
                     /locus_tag="PKV97_08615"
     CDS             complement(6456..7226)
                     /locus_tag="PKV97_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNB05976.1"
                     /translation="MNPPIDISEEAGVRYLHFGSDWIQGAMRIRKPYALELAYTREMM
                     AGLLLRDGLAADGGHWPRKVLIIGLGAASLARFVHHHLPQARIRVVEIAPAVVAAARQ
                     FFKLPTEDARFSVHIGCGAQFVLEHDETWDYVLVDGFDRNARAGVLDTEPFHQAVRAR
                     LSARGIMAVNLFGRARGYKASLQRIVRAFDDRALAFPSCDSGNVVAFGAAGEPIERSV
                     EELRERARQLKAETGLDLLPTVTRLEAAGELPGGVLRM"
     gene            complement(7223..>7510)
                     /locus_tag="PKV97_08620"
     CDS             complement(7223..>7510)
                     /locus_tag="PKV97_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585461.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNB05977.1"
                     /translation="EGPASQSYGIEVAALAGIPAAVIRDAKRRLRALENREIDAGPQA
                     DLFAALPEPEDAPLSHPVLSALADIDPDALSPREALERLYALKRLAGDRNT"
BASE COUNT         1092 a         2475 c         2807 g         1136 t
ORIGIN      
        1 ttggcccgca acatctggcc cgaccgccac ttccccggct gccgcttcgc cgagcgctgt
       61 ccggccgcct tcgaccgctg cgacgaggaa gtccccgtct ggcatgcggt cggcacgcag
      121 gccgtgcgct gccatctgta cgaaggcgtt gcgattccgc gccggttcgt gcgcgagacg
      181 gcgggtgcgg ccgatgtcgt cgccgcacgc ccggcgcaga gcgtgctcga ggtggccgag
      241 ctggcggtgc atttcccggt acgcaagggt ttcctgcgcc gcaccgtcgg gcacgtgaag
      301 gccgtcgacg gcgtgagcct gcgcctgcag cccgggcgca cgctcgcgct ggtgggcgaa
      361 tcgggctgcg gcaagaccac cgtgggcaag gccctgctgc gcctgatcga gcccaccacc
      421 ggccgcctat ggctcgatgg cgagcgctac gacgcactgc cgctctctgc gctgcaaccc
      481 ctgcggcgtg cggtgcagat ggtcttccag gaccccttcg cctcgctcga tccgcgcatg
      541 cgggtgggcg agatcatcga ggagggcctg gctgcgctcg gcgtcggcgc cgaccaccgc
      601 gcgcgccagg cccgcgtgac cgaactgctc gagcgtgtgg ggctgtcggc ggagatgcgc
      661 tggcgctatc cgcacgagtt ctccggcggc cagcgccagc gcatcgcggt ggcgcgtgcg
      721 ctcgcggtgg cgccgcgggt gatcgtgtgc gacgagccca ccagcgcgct cgacgtttcg
      781 gtgcaggcgc agctgctcaa cctgatgcgc gagctgcagc acgagcaagg cctcgcctac
      841 ctcttcatca cccacaacct cgcggtggtg gagtacctcg ccgacgaggt cgcggtgatg
      901 tatctcggtc gcatcgtcga ggagggcccg atcgagcgcg tgctcggcgt gcgtgcccac
      961 ccctacaccc gcgcgctgct cgcggcggtg ccgcggatcg agggcgcggg cggcgcggca
     1021 ccggacgtgg ggggcacagg cgttgcgggc gggagcacga cgacccccgg cacgatcatg
     1081 gccgagctgc cgtcgccgct cgccccgccg agcggctgcc atttccatcc gcgctgccca
     1141 tacgccaccg acgtatgccg gatccgctac cccgcgcccg ccgacctcgg ccagggccac
     1201 cgcgtgcgct gccactggcc gctcgcgctc cgctgacgct ggttttcttg caggagcgac
     1261 gcgcgtcgcg atcgcgcgag cgttcaagcg cctgcgctcg tcgaggcgct gcagtcgcga
     1321 ctggcgtcgc tccttgtgcg gggggcgcga agcgcggtgg gagccccggg actccatgcc
     1381 cccgccgcca ggatcagcgc ttcttgctgc gcttggtgct gcgcttctcg cccgggcgcg
     1441 gcctggcctt gggcgacttg aggtgaggct cgccgctgcg ggcctggctg gtgttgagct
     1501 tgaggcccgg ttggcgggcg gcggtggcgg cgaacgggag caggctggtc acctcgagcg
     1561 caccgtccgg gtcgatgccg tcgggcacca gcagcgaatg gccttcgccc ggctggctgc
     1621 cggggccgag gccgttgagc ctgcgcagct cggccgagct caggccatgg cgggcggcga
     1681 ccgcctcgag ggtctcgccc gcggccatca cgtgcatccg ccattcgccg tcctcctcgt
     1741 cgagctcggc gaggccttgc tgcagctgct cggcgcggtc ggtgggcacg atgaagatct
     1801 gcccgggcgt ggtcaccgcg gggcggttga agcccgggtt gagggcgagg aactcgtcca
     1861 gcggcatgcc ggagagcttc gccgcggtgg cgaggtcgac cccgatcggt gcctcgacgc
     1921 tgacgaagtg cagctcgttg gcgacgtagg gcaactcgaa gtggaagagc tcgggctcgg
     1981 cgacgatgtt cttgatcgcc tgcagcttgg gcacgtagtt ccgcgtctcg tcgggcatgc
     2041 gcaggtgctc gtattcgagc ggcaggccgt cctcggcgtt gcgcttgagc gcgcgctgca
     2101 ccgcgccttc gccccagttg taggaggcga gcgcgaggtg ccagtcgccg tgcatctcgt
     2161 agatgcgctg caggtagtcg agcgcggcgt tggtcgaggc gatcacgtcg cggcgctcgt
     2221 ccacccagtg gtcctgggtg aggttgtagt gcttgccggt cgaggggatg aactgccaca
     2281 ggccggaagc gtgcgcgcgc gagtaggcca tcgggttgta gctgctctcg accatcggca
     2341 gcagcgcgag ctcggtcggc atgccacggc gctcgagttc gtcgacgatg tagtagagat
     2401 agcgcccgcc gcgctcgaag acctgcttga ggaagcgcgg gcggttgagg tagaagctct
     2461 gctggtccat cacccgctgg ctgtcgaggt cgggcatgcc gaagccgcgg cggatgcgat
     2521 cccagatgtc gttggcgtcg cgggtgagat cgagcgtgag cacgcgcgcc gtctcgggcg
     2581 cggacgtcgc taccgcggcg ctagcggcgg tgtcgctggc ggtgagggtg gccggggccg
     2641 ccgtcacgtc acgggcggac gcgggagagg cgagggcggc aagggcgagg gcgcaggcgc
     2701 agacggccag cagcgggcca tgcctgcggc cgccgcgggg ctgggcggca caagaagcgg
     2761 aagaggtcat cggggctcag aagcggtcct tccaggcacg cagcgcggcg aggcattcga
     2821 gcgcatcctg cgggcggctg ccggtgtgcg cggcgacggc ctcgatcacc ccgggctggt
     2881 cgcagcgcag gaaggggttg atcgccagct cgcgggagac ggtgctcggc agggtgggtt
     2941 cgccgcgtgc gcgcagcgtc tcgcagtgcg cggcccaggc gtcgcgttcg acgttctcgg
     3001 gctcggccgc gcgtgcgaag gccaggttgg acagcgtgta ttcgtgggtg cagaacaccc
     3061 gggtgtcggg gggcagggcg gccaggcggg cgagcgagtg cgcgagctgg gtcggcgtgc
     3121 cttcgaagag ccggccgcag ccggcggaaa agagcgtatc cccgcagaac accaggcccg
     3181 gctcgatgtc catgccgacg taggccacat ggccggcggt gtgcccgggc acgtcgagca
     3241 cgcgcagcgc gatggcgggc gcgggcaggt ggattacgtc gccgtcggag acggcatgga
     3301 cgacgcccgc gatcgattcg cgcgcggggc cgtaaaccgg tacacggtgg ctcgccacca
     3361 gcgcggcgat gccgccggtg tggtcggggt ggtggtgggt gaccaggatc gcgccgagca
     3421 cgaggccgtg ctcgcgcagg gcttcctcga ccggggtcgc gtcgcccgga tcgaccacca
     3481 aggcatggcg gccgtcgtgg acgagccaga tatagttatc gcggaaggcg ggaacgggga
     3541 taatgtccat cccgcgaatt gtgaggacct gagtgccgat gtcaatcctc cggctcgagg
     3601 aatggctgca gaccccgcag gggcgctacc tgctcgactg ggagcaggac ggactggacc
     3661 gcctggtggc ggacattttc ggctatcacg cggtgcagct cgggctgtcc ggattcgatt
     3721 tgctgcgcgc caatcgcatg ccgcgccgga tcgattgcgg cccgcagggt cgcgacctgc
     3781 gctgcagcga atacgagctg ccctttgcca ccggcagcct cgaccttgtg ctgctgccgc
     3841 atgtgctcga gttttccgcg cacccgcacc gggtgctgcg cgaggtcgag cgcgtgctgg
     3901 tgcccgaggg cagcgtcgtg atcagcggct tcaacccgct cagcctgtgg ggcctgcgtc
     3961 gcctgatcgc gcgccgcggc gcggaggcgc cgtggagcgg gcattaccgc tcggcgatgc
     4021 gcatccgcga ctggctcaac ctgctcggct tcgagatcca ggagagccgc ttcggctgtt
     4081 acgccccgcc gctgcgcacg ccgcagtggc tggagcgctg gggctgcctc gaccgcgctg
     4141 gctcgcgctg gtggccggtg ctgggcgcgg gctatctgct gcacggggtc aagcgggtgc
     4201 agggcatgcg gctgatcacc ccccactggc gcggccgcaa ggccgcggcc aaacgacttt
     4261 cgccggtcgc ccagcgcgac ggctcgacga ggaacatgca gtaacgatgg aagaagtgga
     4321 tatctatacc gacggcgcct gcagcggcaa tcccggaccc ggcggctggg gcgcgatcct
     4381 gcgctccgga tcgcacgaga aggagatctg gggtggcgag ccggcgacga ccaacaaccg
     4441 catggagctg ctcgcggtga tccgtgccct cgacgcgctc aagcgcccgg tggcggcgcg
     4501 agttcacacc gacagccagt acgtgcagaa ggggatctcg gagtggatcc acggctggaa
     4561 ggcgcggggc tggaagacgg cctcgaagga gccggtcaag aacgccgacc tgtggcgggc
     4621 gctcgacgac gcggcatcgc gccaccaggt gaagtggttg tgggtgcgcg gccacaatgg
     4681 ccaccccgag aacgaacgcg ccgacgcgct cgcgcgccgc ggcgtggacg cggtgcgcaa
     4741 gtccggcgcc gcggtgcaat gctgagcggg agcgtgcaat gagacagatc gtcctcgaca
     4801 ccgaaaccac gggcctcgac tggcgcaacg gcgaccgcgt catcgagatc ggctgcgtcg
     4861 aactgctcga ccgcagcctc accggccgcc actaccacgt ctacatcaac cccgggcgcg
     4921 gcatcgacgc cgaggcggtg gcggtgcacg gcatcaccga ggaattcctc gccgacaagc
     4981 cgaagttcgc cgatatcgtc gccgacttcg aggacttcgt gcgcgacgcc gagctggtga
     5041 tccacaacgc gagcttcgac gtcggcttcc tggatgccga actcgagcgc ctcgggcgcg
     5101 cgaagctggg tgcgctgtgc gccggggtcg tcgatacgct gaagctcgcc aaggagcaaa
     5161 acccgggcaa gaaggcttcg ctcgacgcgc tctgcgaccg ctacgagatc gacaacgcgc
     5221 accgcgaact gcacggcgcg ctgctcgacg cggaactgct cgccgaggtc ttcctggcga
     5281 tgacgcgcgg ccaggaaagt ctcatgatcg ggctcgacga ggaagccgcg ccgcgggagt
     5341 ccgacagcga gggcgcgccc gtgctgcgcg ccgcggtccg ggtgttgcgt gcgagcgccg
     5401 acgaatgcgc cgcccacgat gcagcgctcg ccgacatcgc aaaggcgaac aaggggctgt
     5461 gcttgtggtt gcctccggag ccggcgtcgg cgggatgagc gcctggcggc tgagcccgcc
     5521 gtgacttgcc acgcccctgc agatgcggga gcgtgcgccg gcgctcatgg aaggcgatgc
     5581 aagtcgtgat cagggcgcat gggatctgcg acggcgccat ggaagcgccc gctcgcgact
     5641 cgcatcgctc cgcaggtgcc ccgcagcgca ccggtttcgt gcacgaggac gctgaaggct
     5701 agcgttcgtc gcggcggacc ttctccaggc ccttgaggat gtcgtggcgg gaaatctgcc
     5761 cgatcaggcg cgcgccctcg acgaccggca ggatgtggtg gttcctggca aggaagagtt
     5821 cgatcgcgtc gatcaggctg gcactggcgc ggatcgagac aacgtcgcgg ttcatgaagt
     5881 cttccacctt tccttcgtcg cggcggaaat acgaggcgtc caacgccgcc ttgaggcagt
     5941 ccttctcgga gaggaagccg atgaggcgtc ggttttcgtc gaccaccggc gctccggaga
     6001 ggccgcgctg gagcagcaga cggaccgcct tgagcagatc ggtgtccggc gagaaggcga
     6061 ggtggtcgcc aaccatgtag tccttgacga gaagggagcc gagcatcatg tctccgggga
     6121 atcagtgtgg ttgttcggcc tgcgccgcca tcatgcgggc ctggcgcttc gagcacggct
     6181 tcttgcagcc gaactcgcag tcctgaccca tcgagccgag cccgccacag ctgccggaga
     6241 tcggcttgcg cccgaaaagg acgccaaccg ccatggccag gatcacgagg ccgacgacga
     6301 gcagggtgat cagaaaagtg ttcatggttt gcagcttatc gggttgcgga tgcacggaca
     6361 tccgatcggg tggacaataa cgcgggctgc cgggttctgg ccatggggcc gttcgagcac
     6421 gcgcgccgga tcgacgcaag tcgcgagcgg gcggctcaca tgcgcagcac gccgcccggg
     6481 agttcccccg cggcttcgag gcgcgtcact gtaggcagaa ggtcgagtcc ggtctcggcc
     6541 ttgagctggc gggcgcgttc gcgcagttcc tcgacgctgc gttcgatcgg ctcgccggcc
     6601 gcgccgaagg cgaccacgtt gccgctgtcg caggacggaa aggcgagcgc gcggtcatcg
     6661 aaggcgcgca cgatgcgctg caggcttgcc ttgtagccac gcgcgcggcc gaagaggttc
     6721 accgccatga tgccgcgcgc cgacaagcgc gcacgcacgg cctggtggaa gggctcggtg
     6781 tcgagcacgc cggcgcgcgc attgcggtcg aagccatcga ccagcacgta gtcccaggtc
     6841 tcgtcgtgct cgagcacgaa ctgggcgccg cagccgatgt gcaccgagaa gcgcgcatcc
     6901 tccgtcggca gcttgaaaaa ctggcgtgcc gcggccacca ccgcgggagc gatctcgacc
     6961 acgcggatcc tggcctgcgg caggtggtga tgcacgaagc gcgccagcga ggccgcgccg
     7021 aggccgatga tcagcacctt gcgcggccag tgcccgccgt cggcggcaag gccgtcgcgc
     7081 agcagcaggc cggccatcat ctcgcgggtg taggcgagtt ccagtgcgta cggcttgcgg
     7141 atgcgcatcg cgccctggat ccagtcggag ccgaagtgca ggtagcgcac gccggcttct
     7201 tcggagatgt cgatcggcgg gttcatgtgt tgcggtctcc ggccaggcgc ttgagggcgt
     7261 agaggcgttc cagcgcctcg cgcgggctca gtgcatcggg gtcgatgtcg gcgagcgcgc
     7321 tgagcaccgg gtgcgacagc ggcgcgtcct ccggctcggg cagggcggcg aagaggtcgg
     7381 cctgcgggcc ggcgtcgatc tcgcggttct ccagcgcgcg cagccggcgc ttggcgtcgc
     7441 ggatcaccgc ggccgggatg ccggcgagcg cggcgacctc gatgccgtag ctctggctcg
     7501 ccgggccttc
//
LOCUS       DAOQNN010000133         7475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5166_length_7475_cov_10.876819, whole genome shotgun sequence.
ACCESSION   DAOQNN010000133 DAOQNN010000000
VERSION     DAOQNN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5166_length_7475_cov_10.876819"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKV97_08625"
     CDS             <1..366
                     /locus_tag="PKV97_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNB05978.1"
                     /translation="GEETRSNPWLAELQQEMFAEVNPKDANDAGFRNGEYIWVESPTK
                     ARLKVRAQVTQRVAPGTVFLPFHFSGWWQGKDMLEFYPAGAAPVVRGEAVNTATTYGY
                     DSVTMMQETKTTLCRVSKA"
     gene            385..1023
                     /gene="fdh3B"
                     /locus_tag="PKV97_08630"
     CDS             385..1023
                     /gene="fdh3B"
                     /locus_tag="PKV97_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HNB05979.1"
                     /translation="MGRMKFLCDAERCIECNGCVTACKNEHEVPWGVNRRRVVTMNDG
                     VPGERSISVACMHCSDAPCMAVCPTDCFYKTEDGVVLHDKDKCIGCGYCFYACPFGAP
                     QFPNGPAAFGARGKMDKCTFCAGGPEETGSAAEYEKYGSNRLAQGKLPLCAEMCSTKA
                     LIAGDADVVANIFRERVLRRGTGAEVWGWDAAYGGGERKQERKQERKREGQS"
     gene            1020..1256
                     /locus_tag="PKV97_08635"
     CDS             1020..1256
                     /locus_tag="PKV97_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05980.1"
                     /translation="MSTGKLKSIALATLAGAALLGLSACSEVPQVTVYEQGQYRGKTD
                     ARPWEGGEFKGDRAAWEKALKERSRGQNEYNRIQ"
     gene            1267..2289
                     /locus_tag="PKV97_08640"
     CDS             1267..2289
                     /locus_tag="PKV97_08640"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HNB05981.1"
                     /translation="MTTHAMRGQRGGWLAVLLLALMLWATALPLQAAAPQHGSTAADQ
                     AERQVERPLNNAPVWRAVRSGEEHFTLSRSPEAGVLIQSEGNTWRQLRNGPVTLYGGW
                     LLILVPSAILLFWLVKGTMKLHGQKTGRKMLRFSAFERFVHWGTAISFLLLAVTGVAI
                     LFGKHVLLPLIGHGGLGLLLNAGKLVHNYVGPLFGVFVVLMFFAFLRDNVWRPIDAQW
                     IRRAGGLLDGSHVPSGRFNFGEKTWFWFGVTFLGLTVAISGLIMDFPNLGDWSRADMQ
                     TANIVHAIGAILMLGLAFGHIYMGTLGAEGAYESMKTGYVDETWAREHHEQWYHDVKA
                     GKSGHP"
     gene            2466..3629
                     /locus_tag="PKV97_08645"
     CDS             2466..3629
                     /locus_tag="PKV97_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNB05982.1"
                     /translation="MAETFFSPCPRGLEALLAEELVTFGARAARAVHGGVSWEGDWDA
                     CRRANLESRLATRVLWRVGQGRYRAEVDIYKLAYSVTWAKWFTADDTIRIYVTAQKSP
                     LKSLEFITLRVKDAVCDHFRTVAGRRPNVDTADPAVRIHLFLTVDTATLYVDTTGEPL
                     YKRGYKPAAVEAPLKENLAAGILRLTGWQPDEALVDPMCGSGTFLIEAAQMALDIAPG
                     LGRGFAFERLRIHDRGAWAALRRAAEQRRKPARVLPIFGSDVVGEWVRRSRMNLDAAG
                     LADCVRLDRADVLERTPPAAAGVMVANPPYGVRIGEAEELLAFYPRLGDALKQRWGGW
                     RCYLFSADPELPRHIGLKASRRTPLFNGALECRLFEYRMVAGSNRERTPRPQE"
     gene            complement(3651..4787)
                     /locus_tag="PKV97_08650"
     CDS             complement(3651..4787)
                     /locus_tag="PKV97_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNB05983.1"
                     /translation="MERDQALKFMHDLLRLMLQKNGSDLFITAGFPPAIKVDGRIVPQ
                     SNQILQPQHTTELARAIMNDRQAADFETHKECNFGISPAGIGRFRANAFVQQGRVALV
                     LRTIPQKIPNFKELGLPPVLTEIALAKRGLVIFVGGTGTGKTTSLASLVDYRNENTYG
                     HIITIEDPIEFVHQHKNCIVSQREIGIDTESWMSALKNTLRQAPDVILMGEIRDRETM
                     DYAIAFAETGHLCLATLHANSANQAIDRIINFFPEDRRQQLLMDLSLNLRAMVSQRLL
                     PMKDRKGRVPAVEVMLNSPLIADLVFKGNVPEIKDVMKRSRELGMQTFDQSLFQLYED
                     DLIGYEDALRNADSVNDLRLQIKLNSRHGDPLAAGGGIQNLGIL"
     gene            complement(4829..5872)
                     /locus_tag="PKV97_08655"
     CDS             complement(4829..5872)
                     /locus_tag="PKV97_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNB05984.1"
                     /translation="MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEH
                     KEVHAMVYDIMNDAQRKQLEETWECDFSFAVPNLARFRVNAFQQNRGAGAVFRTIPSK
                     VLTLEELNCPKIFKEIAHQPRGIVLVTGPTGSGKSTTLAAMLDYVNENEYGHILTVED
                     PIEFVHESKRCLVNQREVHRDTMSFNNALRAALREDPDVILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTIDRIVDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGQG
                     RVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQNIGMQTLDQCLVDLVRRNVVSAAE
                     ARVKAQNKDSFAA"
     gene            5999..6712
                     /locus_tag="PKV97_08660"
     CDS             5999..6712
                     /locus_tag="PKV97_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNB05985.1"
                     /translation="MTTIAARLDAVRARIAAAARHAGRDPADVKLLAVSKTWPAEAVR
                     EAAAAGQRAFGENYVQEGVDKVEALRALGLEWHFIGPLQSNKTRPVANAFDWVHGIDR
                     LKIAERLSAQRDVHLPPLNVCIQVNVSGEDSKSGVAPVEAGALAHAVAALPRLRLRGL
                     MCIPEPSADEAVLRARFAVLRGLYDGLRAAGLALDTLSMGMSHDLEPAIAEGASIVRV
                     GTAIFGERARTAPTSTPTA"
     gene            6756..>7475
                     /gene="proC"
                     /locus_tag="PKV97_08665"
     CDS             6756..>7475
                     /gene="proC"
                     /locus_tag="PKV97_08665"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNB05986.1"
                     /translation="MKITFLGGGNMATALIGGMLERGFAVAGIQVVELGEAARCALAQ
                     RFGVRAVESFDDAALACDVLVLAVKPQQMKAALAPIAGRLCGQLVISIAAGLRLADLG
                     RWLGAPGAPHARLVRCMPNTPALIGAGVTGLYADPSVDAAGREAAGRILGAVGSIVWA
                     DTEAQMDAVTAISGSGPAYVFHFIEALESAGRSLGFDEAGARRLAIDTVLGAARLAAG
                     SEDAPAVLRQKVTSKGGTTEAA"
BASE COUNT         1091 a         2510 c         2678 g         1196 t
ORIGIN      
        1 ggcgaggaga cccgctccaa cccctggctg gccgagctgc agcaggagat gttcgccgag
       61 gtgaatccga aggacgccaa cgacgcgggc ttccgcaatg gcgagtacat ctgggtcgag
      121 tcgcccacca aggcgaggct caaggtgcgc gcgcaggtca cccagcgcgt ggcgccgggc
      181 acggtcttcc tgcccttcca cttctccggc tggtggcagg gcaaggacat gctcgagttc
      241 taccccgcgg gtgcggcccc ggtcgtgcgc ggcgaggcgg tcaacaccgc caccacctac
      301 ggctacgact cggtgaccat gatgcaggaa accaagacca ccctgtgccg cgtgagcaag
      361 gcctgaggac gaggagagta agagatggga cgcatgaaat tcctctgtga cgccgagcgc
      421 tgcatcgagt gcaacggctg cgtcaccgcg tgcaagaacg agcacgaggt gccctggggc
      481 gtgaaccgcc gccgggtggt gacgatgaac gacggcgtgc cgggcgagcg ctcgatctcg
      541 gtggcctgca tgcactgctc ggacgcgccc tgcatggcgg tgtgcccgac cgactgcttc
      601 tacaagaccg aagacggcgt ggtgctccac gacaaggaca agtgcatcgg ctgcggctac
      661 tgcttctatg cctgtccgtt cggcgccccg cagttcccca acggcccggc ggctttcggt
      721 gcgcgcggca agatggacaa atgcaccttc tgcgccggcg gccccgagga gaccggctcg
      781 gccgccgagt acgagaagta cggctccaac cgcctcgccc agggcaagtt gccgctgtgc
      841 gccgagatgt gctcgaccaa ggcgctgatc gccggcgacg ccgacgtggt ggccaacatc
      901 ttccgcgagc gcgtgctgcg ccgcggcacc ggcgccgagg tgtggggctg ggatgcggcc
      961 tacggcggcg gtgagcgcaa gcaggaacgc aagcaggaac gcaagcggga ggggcagtca
     1021 tgagtaccgg caagctgaag tcgatcgcgc tcgcgacgct ggccggcgct gcgctgctcg
     1081 gcctgtcggc ctgcagcgag gtgccgcagg tcacggtgta cgagcagggc cagtaccgcg
     1141 gcaagaccga cgcccgcccc tgggaaggcg gcgagttcaa gggcgaccgc gcggcctggg
     1201 agaaggcgct caaggagcgt tcccgcggcc agaacgagta caaccgcatc cagtgaggag
     1261 atcgtcatga cgacacacgc gatgcggggg cagcgcggcg gctggctcgc cgtgctgctg
     1321 ctcgcgctca tgctgtgggc gacggcgctg ccgctgcagg cggcagcgcc gcagcacggc
     1381 agtaccgcgg cggaccaggc cgagcgccag gtcgagcgcc cgctcaacaa cgcgccggtg
     1441 tggcgcgcgg tgcgctcggg cgaggagcac ttcaccctct cgcgcagccc cgaggccggt
     1501 gtgctgatcc agagcgaggg caacacctgg cgccagcttc gcaacggccc ggtcacgctc
     1561 tacggcggct ggctgctcat tctcgtgcct tcggccatcc tgctgttctg gctggtcaag
     1621 ggcacgatga agctgcacgg gcagaagacc gggcgcaaga tgctgcgctt ctcggctttc
     1681 gagcgcttcg tgcactgggg cacggcgatc agcttcctgc tgctcgccgt caccggagtg
     1741 gcgatcctgt tcggcaagca cgtgctgctg ccgctcatcg gccatggcgg gctgggcctg
     1801 ctgctcaacg ccggcaagct ggtgcacaac tacgtcggtc cgctgttcgg ggtcttcgtg
     1861 gtgctgatgt tcttcgcctt cctgcgcgac aacgtctggc ggccgatcga cgcgcagtgg
     1921 atccgccgcg ccggcggcct gctcgacggc agccacgtgc cttcggggcg cttcaacttc
     1981 ggcgagaaga cctggttctg gttcggcgtc accttcctcg gcctcaccgt ggcgatctcc
     2041 ggcctgatca tggacttccc gaacctgggt gactggagcc gtgccgacat gcagaccgcg
     2101 aacatcgtgc atgcgatcgg cgccatcctg atgctggggc tggccttcgg ccacatctac
     2161 atgggcacgc tcggcgccga aggcgcctac gagtcgatga agaccggcta cgtcgacgag
     2221 acctgggcgc gcgagcacca cgagcagtgg taccacgacg tcaaggcggg caagtccggg
     2281 catccctgag cgggctcgcc atctcctctc cctcgtccgg gccgcccatc ccttcggccc
     2341 gggacgcttc ggccccgacc ggcatcgcat gcctgcgggg ccgcgttttg tcggcttccc
     2401 ggggttcgcg ctgcggcgca tgggcccact aggtacaatc cgtttttgtc cgcccggagc
     2461 ctgacatggc cgaaaccttc ttttccccct gcccgcgcgg cctcgaggcg ctgctggccg
     2521 aagagctggt gactttcggc gcgcgcgcgg cgcgcgcggt gcatggcggg gtgagctggg
     2581 agggcgactg ggacgcctgc cggcgcgcca acctcgagag ccggctcgcc acccgcgtgc
     2641 tgtggcgggt ggggcaggga cgctatcgcg ccgaggtcga catctacaag ctcgcctata
     2701 gcgtcacctg ggcgaagtgg ttcaccgccg acgacaccat ccgcatctac gtcaccgcgc
     2761 agaagtcgcc gctcaagagc ctcgagttca tcaccctgcg ggtgaaggac gcggtgtgtg
     2821 accatttccg cacggtggcc ggacggcggc cgaacgtgga taccgccgac ccggcggtgc
     2881 gcatccacct gttcctcacc gtcgacaccg ccacgctgta tgtcgacacc accggcgagc
     2941 cgctctacaa gcgtggctac aagcctgctg cggtggaggc gccgctgaag gagaacctcg
     3001 ccgccggcat cctgcgcctc acaggctggc agccggacga ggccctcgtc gatccgatgt
     3061 gcggcagcgg caccttcctg atcgaggccg cgcagatggc gctcgacatc gcaccgggcc
     3121 tcgggcgcgg cttcgccttc gagcgcctgc gcatccacga ccgcggcgcc tgggcggcgc
     3181 tgcgccgcgc cgccgagcag cgccgcaagc cggcgcgcgt gctgccgatc ttcggttcgg
     3241 acgtggtcgg cgagtgggtg cggcgcagcc gcatgaacct cgacgcggcc gggctggccg
     3301 actgcgtgcg cctggaccgc gccgacgttc tcgagcgcac gccgccggcc gccgcggggg
     3361 tgatggtggc gaatccgccc tacggggtgc gtatcggcga ggcggaggaa ctcctcgcct
     3421 tctatccccg cctgggcgac gcgctcaagc agcgctgggg cggctggcgc tgctacctgt
     3481 tcagcgccga ccccgagctg cccaggcaca tcggcttgaa ggcgagccgg cgcacgccgc
     3541 tcttcaacgg cgcgctcgag tgccgcctgt tcgagtaccg catggtggcc ggcagcaacc
     3601 gcgagcgcac gccgcgtccg caggagtaac aacgcgcgcg gcgtcccgcc ctacaggatg
     3661 ccgaggttct ggatgccgcc gccggccgcg agggggtcgc cgtggcggct gttgagcttg
     3721 atctgcaggc gcaggtcgtt caccgagtcg gcgttgcgca gcgcgtcctc gtagccgatc
     3781 aggtcgtcct cgtagagctg gaacagcgac tggtcgaagg tctgcatgcc gagctcgcgc
     3841 gagcgcttca tcacgtcctt gatctcgggc acgttgccct tgaagaccag gtcggcgatc
     3901 agcggcgagt tgagcatcac ctccaccgcc ggtacgcggc ccttgcggtc cttcatcggc
     3961 agcaggcgct gcgagaccat cgcgcgcagg ttcagcgaca ggtccatcag cagctgctgg
     4021 cggcggtctt ccgggaagaa gttgatgatg cggtcgatcg cctggttggc gctgttggcg
     4081 tgaagggtgg ccaggcacag gtggccggtc tcggcgaagg cgatcgcgta gtccatggtc
     4141 tcgcggtcgc ggatctcgcc catcaggatc acgtcgggcg cctggcgcag ggtgttcttc
     4201 agcgccgaca tccaggactc ggtgtcgatg ccgatctcgc gctgcgagac gatgcagttc
     4261 ttgtgctggt gcacgaactc gatcgggtcc tcgatggtga tgatgtggcc gtaggtgttc
     4321 tcgttgcggt aatcgaccag ggatgcgagc gaggtggtct tgccggtgcc ggtgccgccg
     4381 acgaagatca ccagcccgcg cttggcgagc gcgatctcgg tgagcaccgg cggcaggccg
     4441 agctccttga aattggggat cttctgcggg atggtgcgca gcaccagggc gacgcggccc
     4501 tgctgcacga aggcattggc gcggaagcgg ccgatgcccg ccggcgagat gccgaagttg
     4561 cactccttgt gggtctcgaa gtcggcggcc tggcggtcgt tcatgatcgc gcgcgccagc
     4621 tcggtggtgt gctgcggctg caggatctgg ttcgactgcg gcacgatgcg gccgtcgacc
     4681 ttgatcgcag gcgggaagcc ggcggtgatg aagaggtccg agccgttctt ctgcagcatc
     4741 agccgcagca ggtcgtgcat gaacttgagg gcctggtcgc gttccatggt ctgcgtgctc
     4801 ctgcccggga cgggcgggtg gatgccgctc aggcggcgaa gctgtccttg ttctgcgcct
     4861 tcacgcgtgc ttccgcggcc gacacgacgt tgcgccggac caggtcgacg aggcactggt
     4921 cgagggtctg catgccgata ttctggccgg tctggatcga ggaatacatc tgcgcgatct
     4981 tgttctcgcg gatgaggttg cggatcgccg gggtgccgat catgatctcg tgcgccgcga
     5041 cccggccctg gccgtccttg gtcttgagca gggtctgcga gatcactgcg cgcagggatt
     5101 ccgaaagcat cgagcgcacc atgtccttct cggccgcggg gaagacgtcg acgatccggt
     5161 cgatggtctt ggccgcggat gaggtgtgca gggtgccgaa caccaggtgg ccggtctcgg
     5221 cggcggtgag ggcgaggcgg atggtctcga ggtcgcgcat ctcgcccacc aggatcacgt
     5281 cggggtcctc gcgcagggca gcgcgcagcg cgttgttgaa ggacatggtg tcgcggtgca
     5341 cctcgcgctg gttcaccagg cagcgcttgg attcgtgcac gaactcgatc gggtcctcca
     5401 cggtgaggat gtggccgtac tcgttttcgt tcacgtagtc gagcatcgcc gccagcgtgg
     5461 tggacttgcc cgagccggtc ggcccggtca ccagcacgat gccgcgcggc tggtgggcga
     5521 tctccttgaa gatcttcggg cagttgagct cctccagggt cagcaccttg ctcggaatgg
     5581 tgcggaacac cgcgcctgcg ccgcggttct gctggaaggc gttgacgcgg aagcgcgcga
     5641 ggttgggcac ggcgaaggag aagtcgcact cccaggtctc ctcgagctgc ttgcgctgcg
     5701 cgtcgttcat gatgtcgtac accatggcat gcacctcctt gtgctcgagc ggcggcaggt
     5761 tgatccggcg cacgtcgccg tgcacgcgga tcatcggcgg caggccggcg gagaggtgga
     5821 ggtcggaggc cttgttcttg accgcgaagg cgagcagttc ggtgatgtcc atggtattcc
     5881 ggcgcgaatg gcgacacggc cgcctgcgcg ggcggcgctc ggggtcggcc tcggggtggg
     5941 cgatggagcg atgctatact tttgccgccc cgaaatccca ggaaataggc cacggaccat
     6001 gacgacgatc gctgcacgcc tcgacgcggt gcgcgcccgc atcgccgccg ccgcacgaca
     6061 tgccgggcgc gaccctgcgg acgtgaagct gctcgcggtg agcaagacct ggccggccga
     6121 ggcggtgcgc gaggccgccg ccgccgggca gcgtgccttc ggcgagaact acgtgcagga
     6181 aggcgtggac aaggtcgagg cgctgcgcgc gctcggactc gaatggcatt tcatcggccc
     6241 gctgcagagc aacaagacgc gcccggtggc gaacgccttc gactgggtgc atggcatcga
     6301 ccgcctgaag atcgccgagc gcctgtcggc gcagcgcgac gtgcatctgc cgccgctgaa
     6361 cgtgtgcatc caggtcaatg tcagcggcga ggacagcaag agcggcgtgg cgccggtcga
     6421 ggccggcgcg ctcgcgcacg cggtcgccgc cctgccgcgg ctgcgcctgc gcggcctcat
     6481 gtgcatcccc gaaccgagcg cggacgaggc cgtgctgcgc gcgcgcttcg ccgtgctgcg
     6541 cgggctctac gacgggctgc gcgccgccgg actcgcgctc gacaccttgt cgatgggcat
     6601 gtcgcacgac ctcgagccgg cgatcgccga gggcgcgagc atcgtgcgcg tgggcacggc
     6661 gatcttcggc gagcgtgccc gaaccgcgcc gacctcgacc cctacggcct gatgcgcgtc
     6721 cacgcgcgtc ccggccccaa ccagcaggtt catccatgaa gatcaccttc ctcggcggcg
     6781 gcaacatggc caccgccctc atcggcggca tgctcgagcg cggcttcgcc gtcgccggca
     6841 tccaggtcgt cgaactcggc gaggcggcgc gctgcgcgct cgcgcagcgc ttcggcgtgc
     6901 gtgcggtcga atccttcgac gacgcggcgc tcgcctgcga cgtgctcgtg ctcgcggtca
     6961 agccgcagca gatgaaggct gcgctggcac cgatcgccgg gcgcctgtgc ggccagctcg
     7021 tcatcagcat cgccgccggc ctgcgcctgg ccgacctcgg gcgctggctg ggtgcgccgg
     7081 gtgcgcccca tgcccgcctg gtgcgctgca tgcccaacac cccggcgctg atcggcgccg
     7141 gcgtcaccgg gctgtacgcc gacccctcgg tggacgccgc cggccgcgag gccgccgggc
     7201 gcatcctggg cgcggtcggc agcatcgtgt gggcggacac cgaagcgcag atggacgcgg
     7261 tcaccgcgat ctcgggcagc ggcccggcct acgtcttcca cttcatcgag gcgctcgaat
     7321 ccgccgggcg cagcctcggc ttcgacgagg ccggcgcgcg ccgcctggcg atcgacaccg
     7381 tgctcggcgc ggcgaggctg gcggcgggct cggaagacgc ccccgccgtg ctgcgccaga
     7441 aggtcacctc caagggcggc accaccgagg ccgcg
//
LOCUS       DAOQNN010000134         7471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5173_length_7471_cov_9.274137, whole genome shotgun sequence.
ACCESSION   DAOQNN010000134 DAOQNN010000000
VERSION     DAOQNN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5173_length_7471_cov_9.274137"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1321
                     /locus_tag="PKV97_08670"
     CDS             158..1321
                     /locus_tag="PKV97_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HNB05987.1"
                     /translation="MKFDLIIVGGGLAGAALAVALRRSALRIAVIEHAVPRRPEGWDA
                     RVYAYSPVSARFLDQLGVWAHLDHQRLAEVAEMRIHGDAGGELVFSAYDSGLEELAWI
                     GESSLVHVEIWESLKRQHNVTLFCPAAPEALEIDEDVATLRLGDGRSLKAALVVGADG
                     RDSWVRERAGIAATITPYGERGVVANFVCERPSRGVAYQWFRADGVLAWLPLPGRHMS
                     MVWSAPDAFADELLGLEVEAFCARVEGAGGRALGKLELAGQRAAFPLRLMRVEETVRP
                     RVALIGDAAHAIHPLSGHGINLGFQDAKALAEVLDALPSWQDAGDLSVLRRYARMRAE
                     EPFLLQYTTHALSRLFGRQDSLSATLRNLGMNLTGRVPVLKNALVRYAANGRF"
     gene            1339..2067
                     /locus_tag="PKV97_08675"
     CDS             1339..2067
                     /locus_tag="PKV97_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNB05988.1"
                     /translation="MKALSVPSLIRPLGLALALGATTFAHADEAGVKKSMEAFIGSPA
                     VERVTRTDYAGLYEVVLKNGQLVYTDAKNSFIIDGSIIDTATRRDVTQARLNQLSAID
                     FSTLPLDHAVKVVKGKGTRVIATFEDPNCGYCKRLGKELAQMDDVTVYTFLYPILSED
                     SRIKSDNIWCAKDKGRAWTDWVVDGKEPAKASCDTATITRNVELGQSLRISGTPTIFL
                     ADGSRIGGYLPRAELEKAMDAAKR"
     gene            complement(2112..3131)
                     /locus_tag="PKV97_08680"
     CDS             complement(2112..3131)
                     /locus_tag="PKV97_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HNB05989.1"
                     /translation="MEGWQRDELSAAWPALRASCSTLVRQAPWKAACEEAARLGGAPD
                     GGTVRRFLESRFTPWQVVNPDGSANGLVTGYYEPLIRGSRTRGGRSTWPIHGVPQDML
                     TIELADVYPELKSLRLRGRLVGNKVLPYWNRQQLSERQVPAPVLLWADDPIELFFLQV
                     QGSGRVQLPDGSLVRVGYADQNGHPYQSIGRWLVAQGELTLDKASMDGIKRWAQDNPR
                     RLQELLNANPSYVFFRELPASGGGPVGALGVPLSEGRSIAVDPRYVPLGAPVFLSTTQ
                     PLSERPLERLVMAQDTGGAIKGAVRADFFWGFGAEAGREAGKMRQQGRMWVLLPRGMK
                     PPMPH"
     gene            3433..3567
                     /locus_tag="PKV97_08685"
     CDS             3433..3567
                     /locus_tag="PKV97_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB05990.1"
                     /translation="MWLIFLELGVALALLLVVGLALRQSSAHARDRDDCGNPDDQGTR
                     "
     gene            3548..4423
                     /locus_tag="PKV97_08690"
     CDS             3548..4423
                     /locus_tag="PKV97_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB05991.1"
                     /translation="MTKELAELLARAEGVLARLETLLPGPAAAPDWDAAVAFRWQRRH
                     GRTALRPVRAPHRIMLADIQDVDEQKARIDRNTRQFLAGRRANNVLLTGARGTGKSSL
                     IKALLNEYAARGLRLIEVDKTDLVDLPEIVELVAGRPERFILFCDDLSFEEGEDAYKA
                     LKSVLDGSVSAMSDNVLIYATSNRRHLMPEYHDENLQARHVDGEVHPGEAVEEKISLS
                     ERFGLWISFYPFSQDEYLDIVAHWLRSFGASDDEISAARQEALQWALMRGSRSGRVAW
                     QFARDHAGRLQDDAR"
     gene            4420..5370
                     /locus_tag="PKV97_08695"
     CDS             4420..5370
                     /locus_tag="PKV97_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HNB05992.1"
                     /translation="MTRKRVEVAAGVLLREDGCYLLGQRAPDTVYAGYWEFPGGKVER
                     GESPAQALVRELDEELGIRVTRLRPWLCREHLYEHAHVRLHFHEVAAWEGELADRVHS
                     ALAWVRPEGPAREPMLPANGPILKALRLPRTMGITQAAEIGVEVQLDALERALAGGLR
                     LVQVREATLPDAAREAFARAALARVRAHGGLLVVNEDEVLARRIGADGVHLPARRLMA
                     VQARPDFPWVGASCHDRAELERAAALELDYALLGPVQPTLTHPGQAGMGWEGFASTAA
                     GLPLPVFALGGLAADDMERARDAGAHGIAAIRAIWRPVRD"
     gene            complement(5420..5641)
                     /locus_tag="PKV97_08700"
     CDS             complement(5420..5641)
                     /locus_tag="PKV97_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNB05993.1"
                     /translation="MDTRARTVRCPTCGKDVEWKPESRWRPFCSARCKQIDLGEWASE
                     GYRVPSSPPDAPLDEIDYQPPGAGEMPQR"
     gene            complement(5644..6399)
                     /gene="zapD"
                     /locus_tag="PKV97_08705"
     CDS             complement(5644..6399)
                     /gene="zapD"
                     /locus_tag="PKV97_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HNB05994.1"
                     /translation="MISYEYPLNERIRTLLRLEDLLLKIAHFTAGTAPQDHHVALLTI
                     FETLEVASRADLKVDLVQELERQRQILMSFRNNPEISEEALAGALYEIEQASTGLLAM
                     AGKIGQYLRENEWLMSIRSRAAIPGGVCQFDLPSYHYWLNRDPAVRRGDLGTWIQPIA
                     PIRDGIEIVLRLLRASGHPEPLHARAGAFQLTMAGRTAQMLRVRLAQSETVVPEISAN
                     KYALNVRFMLPETVARPRVAERDVAFELTFCSL"
     gene            complement(6485..7099)
                     /gene="coaE"
                     /locus_tag="PKV97_08710"
     CDS             complement(6485..7099)
                     /gene="coaE"
                     /locus_tag="PKV97_08710"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326473.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNB05995.1"
                     /translation="MSAKRPYIVGLTGGIGSGKSAATDRFAALGASVVDTDRIAHELT
                     APGGGAIEAIHVAFGDAMIAPDGRLDRAAMRALAFREPDARRRLEAILHPLIRAESAR
                     QCLAASGPYVILAVPLLVESGGYRERCDRICVVDCPEALQVARVRARSGLVDEQIRAI
                     MAAQASRAQRLAAADDVLDNAGTLASLHAQVDRLHAAYLAAATH"
     gene            complement(7113..>7471)
                     /locus_tag="PKV97_08715"
     CDS             complement(7113..>7471)
                     /locus_tag="PKV97_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB05996.1"
                     /translation="ARSRSETPEAWWVRIPPQRNRSEAERRVVELNLLGVSDTFIVQE
                     SGPNQNAVSLGLFKTENRARILLGQLRAKGVENAGIEPRMGTSYRIQANVPANELRIV
                     ESAAPGLRARRQACTR"
BASE COUNT         1086 a         2548 c         2688 g         1149 t
ORIGIN      
        1 actaaccaca ccaacacaac gccccacgca cacatccgtg cctggggcgt cgtgctttac
       61 agctgccgga tccccccgca ctccgcgagt gcgtacgtgg cggcaaggcg cgccctcggg
      121 cacaatccaa agctgtaccg agtttccggg gatcgcgatg aaattcgacc tcatcatcgt
      181 cggcggcggt ctcgccgggg ccgcgcttgc ggtagcgctg cgccgtagcg cactgcggat
      241 cgccgtgatc gaacatgctg tgccgcgccg ccccgaaggc tgggacgccc gcgtctacgc
      301 ctacagcccg gtgagcgcac gcttcctgga ccagcttggc gtgtgggccc atctggacca
      361 ccaacgcctc gctgaagtcg ccgagatgcg tatccacggc gatgcgggcg gcgagctcgt
      421 attttcggcc tacgacagcg gactcgagga gcttgcctgg atcggcgagt ccagcttggt
      481 tcatgtcgag atctgggaga gcctcaagcg ccagcacaac gtcacgctgt tctgtccggc
      541 ggcgccggag gcgctcgaga tcgacgaaga tgtggcgacg ttgcgcctgg gcgacggtcg
      601 cagcctgaaa gccgcgttgg tcgtcggtgc ggacggacgc gactcctggg tacgcgaacg
      661 cgccggaatc gccgcgacga tcacgcccta cggcgagcgc ggcgtggtgg ccaacttcgt
      721 ctgcgagcgc cccagtcgcg gtgtcgctta ccagtggttc cgcgccgacg gcgtgttggc
      781 ttggctgccc ctgcccgggc gtcacatgtc catggtgtgg tccgcgcccg acgcgttcgc
      841 cgacgagctg ctcgggctcg aggtcgaggc gttctgcgcg cgcgtggagg gggctggcgg
      901 ccgcgcgctc ggcaagctcg agctcgccgg tcagcgcgcc gcttttccgc tgcgactgat
      961 gcgcgtggaa gagacggtgc gcccaagggt ggcgctgatc ggagatgccg cgcatgcgat
     1021 ccacccgctc tcgggacatg gcatcaacct cggctttcag gatgcgaagg cgctggccga
     1081 ggtgctcgac gcgctaccgt cctggcagga cgcaggcgat ctttcggtgc tgcggcgcta
     1141 tgcgcgcatg cgtgcggaag aacctttcct gctgcagtac acgacgcatg ccctgagtcg
     1201 cttgttcggg cgccaggatt ccctctcggc gaccctgcgc aatctcggga tgaacctgac
     1261 cgggcgggtc ccggtcttaa aaaatgcgct ggtgcgctac gctgccaacg gtcgcttctg
     1321 aaccctgtgg agaatttgat gaaagccctt tccgtcccgt ccctgatccg gcccctcggc
     1381 ctcgccctgg cgctcggtgc gaccacgttc gcacacgccg acgaagccgg ggtcaagaag
     1441 agcatggagg ccttcatcgg ctcgccggcg gtggagaggg tgactcgtac cgactatgcc
     1501 gggctgtacg aggtcgtgct caagaacggc cagctcgtgt atacggatgc gaagaacagc
     1561 ttcatcatcg acggcagcat catcgatacc gccacgcgcc gcgacgtgac ccaggcgcgc
     1621 ctcaatcagc tctccgcgat cgacttctcc accctgccgc tcgaccacgc ggtgaaggtc
     1681 gtgaaaggca agggcacgcg cgtgatcgcc accttcgagg atcccaactg cggctactgc
     1741 aagcgcctgg gcaaggagct cgcgcagatg gacgacgtca ccgtgtacac cttcctgtat
     1801 ccgatcctga gcgaggactc gcgtatcaag tcggacaaca tctggtgcgc gaaggacaag
     1861 gggagggcgt ggaccgactg ggtggtcgat ggcaaggagc ccgccaaggc gagctgcgat
     1921 accgcgacca tcacgcgtaa cgtcgaactc ggccagagcc tccgcatcag cggcacgccg
     1981 acgatcttcc tcgccgacgg ctcgcgcatc ggcggttacc tgccgcgcgc cgaactcgaa
     2041 aaggcgatgg acgccgccaa gcgctgatcc ctcgcagggc gcggacggtc acgcccgcgc
     2101 cgcggatcgg gtcagtgcgg catgggcggc ttcatccccc tcggcagcag cacccacatc
     2161 cgcccctgct gacgcatctt cccggcctcg cggccagcct cggcaccgaa tccccaaaag
     2221 aaatcggcac gcactgcgcc cttgatcgcg cccccggtat cctgcgccat gaccaggcgc
     2281 tcgagcgggc gctcgctgag cggctgcgtg gtcgagagga acaccggtgc gcccagcggc
     2341 acgtagcgcg gatccaccgc gatgctgcgg ccctcggaga gcggcacgcc gagcgcgccc
     2401 accggcccgc cgcccgaggc gggcaactcg cggaagaaga cgtagctcgg gttggcattg
     2461 agcagttcct gcaggcgccg cgggttgtcc tgcgcccagc gcttgatccc atccatcgag
     2521 gccttgtcca gcgtcagctc gccctgggcg accagccagc gcccgatcga ctggtaggga
     2581 tggccgttct ggtcggcata gccgacccgc accaggctgc cgtcgggcag ctgcacccgc
     2641 cccgagccct gcacctgcag gaagaagagt tcgatcggat cgtccgccca cagcagcacc
     2701 ggtgccggga cctggcgttc ggagagctgc tggcggttcc agtacggcag caccttgttg
     2761 ccgacgaggc ggccgcgcag gcgcaggctc ttcagttccg gatacacgtc ggcgagctcg
     2821 atcgtcagca tgtcctgcgg cacgccatgg atcggccagg tgctgcgtcc gccgcgcgtg
     2881 cgactgccac ggatcagcgg ctcgtagtag ccggtcacca gcccgttcgc gctgccgtcg
     2941 ggattcacca cctgccaggg cgtgaagcgg ctctcgagga agcgacgcac agtgccgccg
     3001 tcgggtgctc cgcccaggcg ggcggcctcc tcgcacgctg ccttccacgg tgcctggcgc
     3061 accagggtgc tgcaggaggc gcgcaacgcc ggccaggccg ccgacagctc gtcgcgctgc
     3121 cagccttcga tcgcgcccca gtcgctcgcc tgcagcgcgg ctgcggcagg ggcggccggg
     3181 gcagccggag cgcccggcgt ggcggctggt gcggcgccgg agcagggcgc gcagctcgga
     3241 cagggtacgc aggggcgctc tccggagggt tcgggcgtgc cgggtagggc ggcgcaagcg
     3301 gccaccagca gggcggagag ggaagggaag agggcgttgc gggtgcggca gagcatcttg
     3361 cggggcattg gttgaactcg gctgtctttg ccgccagtat accgggcccc cacaggctgc
     3421 cggagacgga aggtgtggct gatctttctc gaactcggtg tcgcgctcgc gctgctgctg
     3481 gtcgtagggc tcgccctgcg ccagtcgtcc gcgcacgcgc gcgaccggga cgattgcgga
     3541 aaccccgatg accaaggaac tcgctgagct gctcgcccgc gccgaaggcg tgctggcgcg
     3601 cctcgaaacc ctgctgcccg gccctgccgc cgcgcccgac tgggacgccg cggtcgcctt
     3661 tcgctggcag cgccgccacg gccgtaccgc gctgcgcccg gtgcgtgcgc cgcaccgcat
     3721 catgctcgcc gacatccagg acgtggacga gcagaaggcg cgcatcgacc gcaacacccg
     3781 ccagttcctc gccggccggc gcgccaacaa cgtgctcctc acgggcgcgc gcggcaccgg
     3841 caagtcctcg ctgatcaagg ccctgctcaa cgaatacgcc gcgcgcggct tgcgcctgat
     3901 cgaggtggac aagaccgacc tggtcgacct gcccgagatc gtcgaactgg tcgccggccg
     3961 gccggagcgc ttcatcctct tctgcgacga cctttccttc gaggagggcg aggacgccta
     4021 caaggccttg aagagcgtgc tcgacggctc ggtgtcggcg atgtccgaca acgtgctgat
     4081 ctacgccacc tccaaccgcc gccacctgat gccggagtac cacgacgaga atctgcaggc
     4141 ccgccatgtc gatggcgagg tgcacccggg cgaggcggtc gaagagaaga tctcgctctc
     4201 cgagcgcttc ggcctgtgga tctccttcta ccccttcagc caggacgagt acctcgacat
     4261 cgtcgcgcac tggctgcgca gcttcggggc gagcgatgac gaaatctccg ccgcccgcca
     4321 ggaggcgctg cagtgggcgc tgatgcgcgg ctcgcgttcg ggccgcgtcg cctggcagtt
     4381 cgcccgcgac catgccggtc gcctgcagga cgacgcgcga tgacgcgcaa gcgggtggag
     4441 gtcgccgccg gcgtgctgct gcgcgaggac ggttgttacc tgctcggcca gcgcgcgccg
     4501 gatacggtgt atgcgggcta ctgggagttt cccggcggca aggtcgagcg tggcgagagc
     4561 ccagcgcagg ctctggtccg cgaactcgac gaggaactcg gcatccgcgt cacgcggctg
     4621 cggccctggc tgtgccgcga gcacctctac gagcatgccc acgtccgcct gcacttccac
     4681 gaggtcgcgg cctgggaggg cgagctcgcc gaccgggtgc acagcgccct cgcctgggtg
     4741 cgtcccgagg gccccgcgcg cgagccgatg ctgcccgcca acggaccgat cctgaaggcc
     4801 ctgcgcctgc cgcgcacgat gggcatcacc caggcggcgg agatcggcgt agaggtccag
     4861 ctcgacgcgc tcgagcgtgc gctcgctggc ggtctgcgcc tggtgcaggt gcgcgaggcg
     4921 acgctgcccg atgctgcacg cgaggccttc gcgcgcgcgg cgctggcgcg cgtgcgggcg
     4981 catggcgggc tgctggtggt gaatgaggac gaggtgctcg cgcgccgcat cggcgccgac
     5041 ggcgtgcatc tgccggcgcg ccggctgatg gcggtgcagg cgcgaccgga tttcccctgg
     5101 gtgggggcgt cctgccacga ccgcgcggag ctcgagcgcg ccgcggcgct ggagctcgac
     5161 tacgccttgc tcggaccggt gcagccgacc ctgacccatc ccggccaggc ggggatgggc
     5221 tgggaagggt tcgcgagcac ggcggcgggc ctgccgctgc cggtgttcgc cctcggcggg
     5281 ctggcagcgg atgacatgga gcgtgcgcgc gatgccggcg cgcacggcat cgccgcgatc
     5341 cgcgcgatct ggaggcccgt ccgggattga aggcgcacgc gccgcgaggc ggtggcgaat
     5401 ccgcccgcgc cggcggcgtt cagcgctgcg gcatctcgcc cgcgccgggc ggctggtagt
     5461 cgatctcgtc gagcggcgca tcgggcggcg aactcggcac acggtagcct tccgaggccc
     5521 actcgccgag gtcgatctgc ttgcagcgcg ccgagcagaa cgggcgccag cggctctccg
     5581 gcttccattc gacgtccttg ccgcaggtgg ggcagcgcac ggtgcgggcc cgggtgtcca
     5641 tgttcacagg ctgcagaagg tcagttcgaa ggcgacgtcg cgctcggcca cgcgtgggcg
     5701 tgcgacggtc tcgggcagca tgaagcgcac gttgagtgcg tacttgttgg cgctgatttc
     5761 gggcaccacg gtctcggact gcgccagacg cacgcgcagc atctgtgcgg tgcgccccgc
     5821 catggtgagc tggaaggcgc cggcacgtgc gtgcagcggc tcggggtggc cgctggcgcg
     5881 cagcaggcgc agcacgatct cgatgccatc gcggatcggc gctatcggct ggatccaggt
     5941 gccgaggtcg ccgcggcgca cggccgggtc gcgattgagc cagtagtggt aggacggcag
     6001 gtcgaactgg cagactccgc ccgggatcgc cgcgcggctg cggatgctca tcagccactc
     6061 gttctcgcgc aggtactggc cgatcttgcc tgccatcgcg agcagcccgg tggaggcctg
     6121 ctcgatctcg tagagcgcgc cggcaagcgc ttcctcggag atctcggggt tgttgcggaa
     6181 ggacatcagg atctggcgct ggcgctcgag ttcctgcacc aggtcgacct tcaggtctgc
     6241 gcggctcgcc acttcgagcg tctcgaagat ggtcagcagc gcgacatggt ggtcttgcgg
     6301 cgccgtgccc gcggtgaaat gggcaatctt gaggagcagg tcctccaggc gcagcaaggt
     6361 gcggatgcgc tcgttgagag gatattcgta gctaatcacc gcagcgtgtc cgcctgtatc
     6421 gcacctgttc agagtcgcgc cgatgatagc acaggccccc ttcccaccat ggcgaggcac
     6481 ggtttcagtg cgtcgccgcg gcgaggtagg cggcgtgcag gcgatcgacc tgtgcgtgca
     6541 gggatgcgag cgtgccggcg ttgtcgagca cgtcgtcggc ggcggccaga cgttgcgccc
     6601 gactggcctg cgcggccatg atggcgcgga tctgttcatc gacgagcccg ctgcgcgcac
     6661 gcacgcgcgc gacctgcagc gcctcgggac agtcgaccac gcagatgcgg tcgcagcgct
     6721 cgcggtagcc gccagactcg accagcagcg gcactgcgag gatcacgtag gggccgctgg
     6781 ccgccaggca ctggcgggcg ctctcggcgc ggatcagcgg gtgcaggatg gcctcgaggc
     6841 ggcggcgggc atcgggctcg cggaaggcga gcgcgcgcat ggcggcgcgg tcgaggcggc
     6901 catcgggcgc gatcatggcg tcgccgaagg cgacgtggat ggcctcgatc gcgccgccgc
     6961 cgggggcggt gagctcgtgg gcgatacggt cggtgtcgac cacgctcgcg ccgagggcgg
     7021 cgaagcggtc ggtggcggcg ctcttgccgc tgccgatgcc gccggtgagg ccgacgatgt
     7081 aggggcgctt cgcgctcatc tccttctccg aatcagcgcg tgcaggcctg gcgccgggcg
     7141 cgcaggccgg gcgcggccga ttcgacgatg cgcagctcgt tggccgggac gttggcctgg
     7201 atgcggtagc tggtgcccat gcgcggctcg atgccggcgt tctcgacgcc cttggcacgc
     7261 agttggccga gcaggatgcg ggcgcggttc tcggtcttga agaggccgag cgagaccgcg
     7321 ttctggttgg ggccggactc ctgcacgatg aaggtgtcgc tgacgccgag caggttgagc
     7381 tcgacgacgc ggcgctcggc ctcgctgcgg ttgcgctgcg gcgggatgcg cacccaccag
     7441 gcctccggcg tctcgctgcg gctgcgcgcc g
//
LOCUS       DAOQNN010000135         7436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5219_length_7436_cov_7.564287, whole genome shotgun sequence.
ACCESSION   DAOQNN010000135 DAOQNN010000000
VERSION     DAOQNN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5219_length_7436_cov_7.564287"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PKV97_08720"
     CDS             complement(<1..1028)
                     /locus_tag="PKV97_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNB05997.1"
                     /translation="MIPVLTAFIGILALAWGLNVAALDGGSASLPWLVREQGLYLSGL
                     LAIGLMSLAMVLATRPTVLERPLGGMDRIFRLHKWAGILAAVFAALHWLIEMSDDVIK
                     SLYGKAGKPAHDEFGGLYEMLRDAGEELGEFAIYLVLAMVVLSLWKRFPYKFWRHLHR
                     AMPVFYLLLAFHAAVLAPPDYWTQPVGVLLALLLAAGTVASVIALTGRIGHDRKVQGV
                     VTAVSSPAPDVTEIVCRLDGAWRGHQAGQFAFVGLERFEGSHPFTIASADHGDRSLTF
                     QIKALGDYTRQLASRVAVGQAVTVEGPYGRFVLGRQDHKARQIWIAGGIGVTPFLAWL
                     DALRADPAA"
     gene            complement(1100..1429)
                     /locus_tag="PKV97_08725"
     CDS             complement(1100..1429)
                     /locus_tag="PKV97_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNB05998.1"
                     /translation="MRATTLIATLALTGGLIGAGATIAPAFAQNAAPAATPAAATAPA
                     AQSNWMSPKDVQTKLEGAGYTDFREIERDKNKYEVKATDPQGQRVEMDVDPVTGDILK
                     TEVKRSK"
     gene            complement(1580..2464)
                     /locus_tag="PKV97_08730"
     CDS             complement(1580..2464)
                     /locus_tag="PKV97_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB05999.1"
                     /translation="MPATPFTALACPLDGAPLQRQGGSLHCPEGHCFDLAAQGYVNLL
                     PVQNKRSRDPGDSKEMVAARRRFLEAGHYKPIADAVAQAVLAGAVPGRTLTCLDAGCG
                     EGYYLRQLAAAAVVEARTLALAGLDISKWAVQAAAKRDLQDARSQAGGAPSATWIVGS
                     NAGLPVLPATLDRVLCMFGFPVYPEFARVLRRGGELLQVDAGPDHLRELREVIYPTLK
                     PERPAARQAPAGFEHVGTDTLRFPIHVDGAAAIADLLAMTPHLYRASAEGRARAAALS
                     ALTMTVDVRLARYRRQPG"
     gene            2584..4191
                     /locus_tag="PKV97_08735"
     CDS             2584..4191
                     /locus_tag="PKV97_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB06000.1"
                     /translation="MHASETLLNTLRRPGVLPGSEGGVELIETHISWVLLAGEHAWKL
                     KKPLDLGFLDFSTPQRRREACEAELRLNRRTVPEIYEAVVAVRGSPEAPRLDGGGEPF
                     DWLLRMRPFDQSGLLSRLLDEGRLAPALFDRLARHVAEFHAAAAVAVPGGGYGDAAAV
                     HAPVRQNFAQMREHLDPGVHADLLAMLARVEAWAEAQYAALAPVFAVRLAEGRVRECH
                     GDLHLGNLVVLDGEPRLFDAIEFSAELRWTDVVADVAFLVMDLQARGRPELGWRFLNA
                     WLERCGDYAGLRVLPYYLSYRAMVRAKIAAIRASQLDGAARSESVDECRRYLALAEAQ
                     RAPGAPALLVACGVAGAGKTSQSQALVEAGGVIRVRADVERKRLAGLAAEATSGSGLG
                     GGLYTPEATARTYARLAVLARAVLEAGRPVLVDATFLKRAQRTAFAGLAQELGVPFAI
                     LAFDAPEDVLRARVRARRAAGDDASEADEAVLEAQLRSREGFDAEELARVLPIDTRSV
                     PDWRSLLPALSRLWPGAPRMDSRPSTH"
     gene            4267..>4676
                     /locus_tag="PKV97_08740"
     CDS             4267..>4676
                     /locus_tag="PKV97_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNB06001.1"
                     /translation="MDTLTHALSGALVGRMLASRRPAAGTRAANAAPVPVWQMVVAGT
                     AAAIFPDLDFVLGWISELTYLRGHRGVTHSLILLPLWGVLIAWLLARFWQRGGRVAAG
                     WRSFYPVVCAAIFVHILGDLITQFGTMILAPFSD"
     assembly_gap    4677..4776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4777..5513
                     /locus_tag="PKV97_08745"
     CDS             <4777..5513
                     /locus_tag="PKV97_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNB06002.1"
                     /translation="GDLITQFGTMILAPFSDHRFGWGTSFIIDLPFTGILLAGLLASA
                     LWRSSRVPATAALALLAGWVGVQATGRAEAIDAARAYAAAKGIAVVEVDAAPRPASPF
                     NWTAIVFDGERYHYAHLNTRRSEPLLATEDDNFIRRFSAPYLPVAMAQWEVRERFGTG
                     STRALAEQVWNAEDFAFYRWFAMFPVLDHAGEAGDGQACASFKDLRFLTPGRDAQPFI
                     YGLCNAAGGWRLFEREAGGLRWIDPR"
     gene            5510..6142
                     /locus_tag="PKV97_08750"
     CDS             5510..6142
                     /locus_tag="PKV97_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HNB06003.1"
                     /translation="MTKPTDDMGDSRPAPAPHAEAEGAGARSPANAECETAKPARKPP
                     RWRRWGVEIGIFVVIFFAVQAWMARDVPAGAAPDFATVAADGCGLSLAEWRAAHPGKA
                     VGVYFWADWCPICTAQQGTIDGVQADHPVLTVAMQSGGAAEVTGVLAERGLAWTTAID
                     ADGRIAEAYGLRGVPAFVIVDPAGKIRSVSLGYATGWGLRARLWWAGLQS"
     gene            6191..6487
                     /locus_tag="PKV97_08755"
     CDS             6191..6487
                     /locus_tag="PKV97_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HNB06004.1"
                     /translation="MKAIPLIRRRVVLAPDAFAEIAVWRVPEPVPPSGHPFKYRLAYV
                     VGGECVLRYDNERGKGDHRHYGDTEAEYVFSDPDQLTRDFGADIARWNHEHGRS"
     gene            6468..6815
                     /locus_tag="PKV97_08760"
     CDS             6468..6815
                     /locus_tag="PKV97_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNB06005.1"
                     /translation="MNTVVLEVRSLTDTLADAARAMKTGRGDAQVRIAFATPELLWKV
                     LSAKRWELLKALCAAGPVSIREAARRVGRDVKAVHGDVTALLDAGVLLRTEDGRIEFP
                     FDAVKVEFLLQAA"
     gene            6828..>7436
                     /locus_tag="PKV97_08765"
     CDS             6828..>7436
                     /locus_tag="PKV97_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB06006.1"
                     /translation="MGAPGLIAAFTAGAATLAGGRALVHWGIRKGLAAPRVPHHTDPG
                     ALGLAFETLRIGTEHGKSLHAWFIPAPGSACDGSDHAASPGADLADGERDDARAEFES
                     APAVVVMHGWGGNAALMLPLARPLHEAGYAMLFVDARCHGASDDDSFASLPRFAEDAE
                     HAFAWLAAQPGVDPARIALLGHSVGAGAMLFAASRTPQVAAVV"
BASE COUNT          952 a         2513 c         2769 g         1102 t
ORIGIN      
        1 gcggcggggt cggcgcgcag ggcgtcgagc caggccagga agggggtgac gccgatgccg
       61 ccggcgatcc agatctggcg cgccttgtgg tcctggcggc cgagcacgaa gcggccgtaa
      121 gggccttcga cggtgaccgc ctggccgacc gcgacgcggc tggcgagctg gcgggtgtag
      181 tcgcccaggg ccttgatctg gaaggtgagg ctgcggtcgc cgtggtcggc gctggcgatg
      241 gtgaaggggt gcgagccttc gaagcgctcg aggccgacga aggcgaactg cccggcctgg
      301 tgaccgcgcc aggcgccgtc gagccggcac acgatctcgg tgacgtcggg ggcgggcgag
      361 gacactgcgg tgaccacgcc ctgcaccttg cggtcgtggc cgatgcggcc ggtgagcgcg
      421 atgaccgagg ccaccgtgcc cgcggcgagc agcagcgcga gcagcacgcc gaccggctgc
      481 gtccagtagt cgggcggcgc cagcacggcg gcgtggaagg cgagcagcag gtagaacacc
      541 ggcatcgcgc ggtggaggtg gcgccagaac ttgtagggga agcgcttcca caggctgagc
      601 acgaccatcg ccagcacgag gtagatggcg aactcgccga gctcctcgcc ggcgtcgcgc
      661 agcatctcgt agaggccgcc gaattcatcg tgcgctggct tcccggcctt gccgtacagc
      721 gacttgatga cgtcgtcgga catctcgatc agccagtgca gcgcggcgaa caccgcggcc
      781 aggatgccgg cccacttgtg caggcggaag atgcggtcca tgccgccgag cgggcgctcg
      841 agcacggtgg gacgggtggc gagcaccatc gccagcgaca tcaggccgat ggcgagcagg
      901 ccgctgaggt agaggccctg ctcgcggacg agccagggca gggaggcgct gccgccgtcg
      961 agggcggcga cgttgaggcc ccatgcgagg gcgaggatgc cgatgaaggc ggttaggacg
     1021 gggatcatgg cggggcctcc cgagtggctg gaatgaagaa aggggcgccg gcagtgccgt
     1081 cgccccctcg cgcaagcgct tacttgctgc gcttgacctc ggtcttgagg atgtcgccgg
     1141 tgaccggatc gacatccatc tccacgcgct gtccttgcgg gtcggtggcc ttgacctcgt
     1201 acttgttctt gtcgcgctcg atctcgcgga agtcggtgta gccggcgcct tcgagcttgg
     1261 tctgcacgtc cttcgggctc atccagttgg attgggcggc gggggcggtc gcggcggccg
     1321 gggtggctgc gggggctgcg ttctgggcga acgcgggggc gatggtggcg ccggcgccga
     1381 tgagtccgcc ggtgagggcc agggtggcga tgagagtggt ggcacgcatg atggcatctc
     1441 ctgtgtcggt tgggcggtcc tgttgcgacc gtgatgccat gctgcgccgc gctggctgaa
     1501 tcgatgctga agatgccgtt catccgccgt tcatgtcgag cgggcgctgc aggtgcggat
     1561 tgccttgcgc ggtttggctt cagccgggct ggcggcggta gcgcgccagg cgcacgtcca
     1621 ccgtcatcgt cagcgcgctg agcgccgccg cgcgggcgcg gccttcggcg ctggcgcggt
     1681 agaggtgggg cgtcatcgcc agcaggtcgg cgatggcggc ggcgccgtcc acgtggatcg
     1741 ggaagcgcag ggtgtccgtg ccgacgtgct cgaagcccgc cggcgcctgg cgtgcggcag
     1801 ggcgttcggg cttgagcgtg gggtagatga cctcgcgcag ctcgcgcagg tgatcggggc
     1861 cggcgtcgac ctgcagcagc tcgccgccgc ggcgcagcac gcgggcgaat tccgggtaga
     1921 ccggaaagcc gaacatgcac agcacgcggt cgagcgtggc cggcagcacc ggcagaccgg
     1981 cgttgctgcc gacgatccag gtggcgctcg gggcgccgcc ggcctggctg cgggcgtcct
     2041 gaaggtcgcg cttcgccgcc gcctgcaccg cccacttcga gatgtccaga ccggcgagcg
     2101 cgagcgtgcg cgcttccacc accgccgccg cggcgagctg gcgcaggtag tagccttcgc
     2161 cgcagccggc gtcgaggcag gtgagcgtgc gccccggcac cgcgccggcg agcaccgcct
     2221 gcgccaccgc gtcggcgatc ggcttgtaat gcccggcctc caggaagcgc cggcgcgcgg
     2281 cgaccatctc cttgctgtcg ccggggtcgc gcgagcgctt gttctgcacc ggcagcaggt
     2341 tcacatagcc ctgggcggcg aggtcgaagc agtgcccctc ggggcagtgc aggctgccgc
     2401 cctggcgttg cagcggcgcg ccgtcgagcg ggcaggcgag ggcggtgaag ggcgtggcgg
     2461 gcatggtcgg cggtcgtcga tggaggcgga cgcgatgata ggggaggcgg cggcgcggcg
     2521 gcgagcggcc cggctgcgcg cgtcccgggc taccatcggg cggatgaccg aggagagctg
     2581 ccgatgcatg catccgaaac cctgctgaac acgctgcgcc ggcccggcgt gctgcccggc
     2641 tcggagggcg gcgtcgagct gatcgagacc cacatctcct gggtgctgct cgccggcgag
     2701 cacgcctgga agctgaagaa gccgctcgac ctcggcttcc tggacttttc cacgccgcag
     2761 cgccggcgcg aggcctgcga ggcggagctg cggctcaacc gccgcacggt gccggagatc
     2821 tacgaggcgg tggtggcggt gcgcggcagc cccgaggcgc cccgcctcga cggtggcggc
     2881 gagcccttcg actggctgct gcgcatgcgc cccttcgacc agtccgggct gctctcgcgc
     2941 ctgctcgacg agggcaggct ggcgcccgcg ctcttcgatc gcctcgcccg ccacgttgcc
     3001 gagttccacg ccgccgccgc ggtggcggtg ccgggcgggg gctatggcga cgcggccgcg
     3061 gtgcatgcgc cggtgcggca gaacttcgcg cagatgcgcg agcacctcga tcccggcgtc
     3121 catgccgacc tgctcgcgat gctggcccgg gtggaggcct gggcggaggc gcagtacgcc
     3181 gcgctcgcgc cggtgttcgc cgtgcgcctg gccgaggggc gggtgcgcga atgccatggc
     3241 gacctgcacc tgggcaacct ggtcgtgctc gacggcgagc cgcgcctctt cgacgcgatc
     3301 gagttcagcg ccgagctgcg ctggaccgat gtcgtcgccg acgtcgcctt cctggtcatg
     3361 gacctgcagg cgcgtgggcg gcccgagttg ggctggcgct tcctgaacgc ctggctggag
     3421 cgttgcggcg actacgccgg cctgcgcgtg ctgccctact acctcagcta tcgcgcgatg
     3481 gtgcgcgcca agatcgccgc gatccgcgcc agccagctcg acggcgccgc acgcagcgag
     3541 agcgtggacg aatgccggcg ctatcttgcg ctcgccgagg cgcagagggc gccgggcgcg
     3601 ccggccttgc tggtggcctg cggcgtcgcg ggcgcgggca agaccagcca gtcccaggcc
     3661 ctggtggagg cgggcggggt gatccgcgtg cgcgccgacg tcgagcgcaa gcgcctcgcc
     3721 ggcctggcgg cggaggcgac gagcggctcc ggcctcggcg gtgggctata taccccggag
     3781 gccaccgcgc gcacctatgc gcgcctggcc gtgctggcgc gcgcggtgct cgaggccggg
     3841 cgcccggtgc tggtcgacgc caccttcctg aaacgcgccc agcgcacggc cttcgccggg
     3901 ctcgcgcagg agctcggcgt acccttcgcg atcctcgcct tcgatgcgcc ggaggacgtc
     3961 ctgcgcgcgc gcgtgcgcgc tcgccgggcc gccggcgacg acgcctcgga ggccgacgag
     4021 gcggtgctcg aggcgcaact gcgcagccgc gagggcttcg atgccgagga actcgcgcgc
     4081 gtcctgccga tcgatacccg gtcagtgccg gattggcgca gcctgctgcc ggcgctgtcg
     4141 aggctgtggc cgggcgcgcc ccggatggac agccggccct ccacccactg aacgcgcccc
     4201 agccgcgcga ccggcccgcc ttggcacggc agccggacaa ggcaacccag agaacccctg
     4261 tacccgatgg ataccctcac ccacgccctc tccggcgccc tcgtcggccg catgctggcc
     4321 tcgcgtcggc ctgctgccgg cacccgggcg gcgaacgcag cgccggtgcc ggtctggcag
     4381 atggtggtcg ccggcaccgc ggcggcgatc ttccccgacc tcgacttcgt gctcggctgg
     4441 atctccgagc tcacctacct gcgcggccac cgcggcgtca cccactcgct catcctgctg
     4501 ccgctgtggg gcgtgctgat cgcctggctg ctggcgcgct tctggcagcg cggcggacgg
     4561 gtggcggcgg gctggcgcag cttctatccg gtggtgtgcg cggcgatctt cgtgcacatc
     4621 ctcggcgacc tcatcaccca gttcggcacc atgatcctgg cgcccttctc cgaccannnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcgg cgacctcatc acccagttcg
     4801 gcaccatgat cctggcgccc ttctccgacc accgcttcgg ctggggtacg agcttcatca
     4861 tcgacctgcc cttcaccggc atcctcctcg ccggcctgct cgcgagcgcg ctatggcgca
     4921 gcagccgcgt gccggcgacg gcggcgctgg cgctgctcgc cggctgggtg ggcgtgcagg
     4981 cgacggggcg cgccgaggcg atcgacgccg cgcgcgccta cgccgccgcc aagggcatcg
     5041 ccgtggtcga agtggacgcc gcgccgcgcc cggcctcgcc cttcaactgg accgcgatcg
     5101 tgttcgacgg cgagcgctac cactacgccc acctcaacac ccggcgcagc gaaccgctcc
     5161 tcgccaccga ggacgacaac ttcatccgcc gcttctccgc gccctacctg ccggtggcga
     5221 tggcgcagtg ggaggtgcgc gagcgcttcg gcaccggcag cacgcgcgcg ctcgccgagc
     5281 aggtatggaa cgcggaggac ttcgccttct accgctggtt tgcgatgttc ccggtgctcg
     5341 accacgccgg cgaggcgggc gacggccagg catgcgccag cttcaaggac ctgcgcttcc
     5401 tcacccccgg gcgcgatgcg cagcccttca tctacggcct gtgcaatgcc gccggcggct
     5461 ggcgcctgtt cgagcgtgag gccggcgggc tgcgctggat cgatccgcga tgacgaagcc
     5521 gaccgacgac atgggggatt cacgacctgc gccggcgccg catgcggaag cggagggcgc
     5581 cggcgcacgc tcgcccgcga acgccgagtg cgaaacggcc aagcccgccc gcaagccacc
     5641 gcgctggcgg cgttggggcg tggagatcgg catcttcgtc gtcatctttt tcgcggtgca
     5701 ggcctggatg gcgcgcgacg tcccggcggg cgctgcgcca gatttcgcca ccgtcgccgc
     5761 cgacggctgc gggctcagcc tcgccgaatg gcgcgccgcg catccgggca aggcggtcgg
     5821 agtgtatttc tgggccgact ggtgcccgat ctgcaccgcg cagcagggca cgatcgatgg
     5881 cgtgcaggcg gaccacccgg tgctcaccgt cgccatgcag tcgggcggcg cggcggaggt
     5941 gacaggcgtg ctcgccgagc gcggcctggc ctggaccacc gccatcgacg ccgacggccg
     6001 cattgccgag gcctacggcc tgcgcggcgt gccggccttc gtcatcgtcg acccggcggg
     6061 caagatccgc tcggtgtcgc tcggctacgc caccggctgg ggcctgcgcg cgcggctgtg
     6121 gtgggcgggt ctccagagct gaatgttgtt gtctttgaca acattcttag atgttgtcaa
     6181 tatgtccaac atgaaagcga ttcccttgat ccgccgtcgg gtcgtgctcg cgccggatgc
     6241 ttttgcagag atcgccgtgt ggcgggttcc ggagccggtg ccgccttccg ggcatccgtt
     6301 caagtaccgc ctggcctacg tggtcggtgg cgagtgtgtt ctgcgctacg acaacgagcg
     6361 cggcaagggc gatcaccggc attacggcga cacggaagcg gaatacgtct tttccgatcc
     6421 ggatcaactc acccgtgact tcggagcgga cattgcgagg tggaaccatg aacacggtcg
     6481 ttcttgaagt gcgcagcctg acggacacgc tggcggacgc cgcgcgggca atgaaaaccg
     6541 ggcggggtga cgctcaggtg cggatcgcct tcgcgacacc cgaactgctc tggaaagtct
     6601 tgagcgccaa gcgctgggag ttgctgaagg ccttgtgtgc ggcgggtccg gtgtcgatcc
     6661 gcgaggccgc gcgccgggtc gggcgcgacg tcaaggcggt gcatggtgac gtcaccgccc
     6721 tgctcgatgc cggcgtgctg ttgcgcactg aagatgggcg gatcgagttt ccgttcgatg
     6781 ccgtgaaggt cgagtttctg ctgcaggcgg cctgaggagc aaacggcatg ggcgcacccg
     6841 gactgatcgc agccttcacc gccggcgcgg ccacgctcgc cggcgggcgg gcgctggtgc
     6901 attgggggat ccgcaagggt ctggcggcgc cgcgggtgcc gcatcacacc gatccgggcg
     6961 cgctcgggct ggcgttcgag acgctgcgca tcggcaccga gcatgggaag tcgctgcatg
     7021 cgtggttcat tccggcgccg gggagcgcct gtgatggatc ggatcacgct gcaagcccgg
     7081 gcgcggacct tgcggacggc gagcgcgatg acgctcgtgc cgagttcgaa tccgcaccgg
     7141 ccgtggtggt gatgcacggc tggggcggca atgcggcgct gatgctgccg ctcgcccgcc
     7201 cgctgcacga ggccggctac gcgatgctgt tcgtcgacgc gcgctgccac ggcgcaagcg
     7261 acgacgacag cttcgcctcg ctgccgcgct tcgccgagga cgccgagcac gccttcgcgt
     7321 ggctggcggc gcagcccggg gtggatccgg cgcgcatcgc gctgctcggc cattcggtcg
     7381 gggcgggcgc gatgctgttc gccgcctcgc gcacgccgca ggtggcggcg gtggtg
//
LOCUS       DAOQNN010000136         7416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5245_length_7416_cov_8.486075, whole genome shotgun sequence.
ACCESSION   DAOQNN010000136 DAOQNN010000000
VERSION     DAOQNN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5245_length_7416_cov_8.486075"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PKV97_08770"
     CDS             complement(<1..614)
                     /locus_tag="PKV97_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HNB06007.1"
                     /translation="MSDATPVPLSPARLVSFRFHGELAAFLAPARRDRTFEVRCAADS
                     SLKHVIEALGVPHTEVGRVLVDGRPAPLERRLCPGERVSVHPHAARCGKPCPSPPPRL
                     IADAHLGGLARLLRLAGFDTLYDNHIEDATLVATAVRERRVVLTRDRELLKRRDVAEG
                     CFVHALEPARQAVEILRRLGLAGHARPFSRCLECNTALHEVSPAA"
     gene            713..964
                     /locus_tag="PKV97_08775"
     CDS             713..964
                     /locus_tag="PKV97_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06008.1"
                     /translation="MKIRALFVTLSGLSAAAVAVLLLAAVPAPAGAIAQPVGPAMPEE
                     CVCSRGLRLAEAPPSVLHNCQCGPLQCVVHAQSGQLQCR"
     gene            978..1403
                     /locus_tag="PKV97_08780"
     CDS             978..1403
                     /locus_tag="PKV97_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB06009.1"
                     /translation="MKPRLSVITLAVDDLERAVAFYRDGLGWPTQGIVGSAAQHSAVA
                     FFALQPGIRLALFPRTSLAVEAGVAEPPSGTSGCLLSHNVARREQVAEVMAEAAVAGA
                     RILRPPRDTAWGGHAGYFADLDGHPWEVAWNPKWPLADD"
     gene            1553..2746
                     /locus_tag="PKV97_08785"
     CDS             1553..2746
                     /locus_tag="PKV97_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB06010.1"
                     /translation="MPSSRRFVLSLSLVGLAALGAYAWYANRPPALPPGGARAQAQAA
                     GVQAVPPAAGTAGAKGAAGAKKGAAGAAGGSQGGPVVVETHTVGVRALADDVGAVGSL
                     VSNESVVLRPEVSGRIEAIRFRDGEAVRRGEVLIEFDAAVQRAELQQARASLTLAESN
                     FRRSQDLFSRSFVSRSSLDNARSQLEVARAGVALAQARLARMQIRAPFAGVVGIRSVS
                     PGDFVKDGEAMINLEDIATLKVDFRLPELYLDRVRPGQTVEIASDVLAGERFAATVEA
                     IDPLVDAQGRALRLRASLPNPELRLRPGVFVRVRLILAERADVVVVPEAALVPAPGNT
                     QFVYRVAEGKAQRVEVRTGTRRDAMVEVVEGVQAGEVVVTAGQLKLRDGAAVKLATGG
                     VARAD"
     gene            2750..5821
                     /locus_tag="PKV97_08790"
     CDS             2750..5821
                     /locus_tag="PKV97_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB06011.1"
                     /translation="MVLSELCIRRPVFATVLSLLVLLVGLMSYSRLTVREYPNIDEPV
                     VTVDTRYRGASAEIVESQVTKPLEDSLAGIEGVDVLSSISRQERSQITVRFRVERSAD
                     SAAADVRDRVSRVRQRLPDDIDEPVIAKVEADASPIIWVAFSSDRHAPMEISDFANRV
                     IKPRLQTLPGAADARLFGDRRSAMRVWLDRDRLAAFGLTVQEVEDAIRRQNVELPAGR
                     IESREREFAVVAQTDLARPEQFGAIVLRQGDTADAYPVRIRDVGRVEVAPESERTSVR
                     FMGRPAVSIGLIKQSVANPLDLKRGLDEMMPRLQAELPEGMSMTIANDTTVFIERSID
                     AVFSTIWEAVLLVAAVCLFFLRNWRAMLVPLVTIPVSLVGAFTLMLVLGFSINTLTLL
                     ALVLAIGLVVDDAIVVLENIYRHIEDGMEPVAAAFKGAREIGFAVVAMTITLAAVYAP
                     VAFMSGRTGKLFTEFALTLAGAVIVSGFVALTLSPMMCSKLLRHEPTHGRIYNGIERF
                     LAWLTGGYRAVLELALGARWLVMLVFAVVAGSSVWLMGQLKSELAPLEDRGRVIGMFT
                     GPEGATLDYMARYAEEIERIYAGLPEVDRYLVIAGNPTVSQGISFVGFTDWSERTRTA
                     GEISSGLQPKLRAVPGVNAFPIMPASFGQSARSRPVSFVVATSESYDALARVTDTFLD
                     AMRDNPGFLSPDTDLKLTMPELAVEVDRDRAADLGVDVDVIARTLESMLGGRQVTRYK
                     QDAEQYDVIVQVGAGERRDPRDIRDIYVRARTGEMVPLTSVVQVSERVAPRELNHFGQ
                     RRSVTISANLAPDYALGEALQWMDDTAARILPPGYSTDYDGQSREFKTSAESLALVFL
                     LALAFIYLVLAAQFESFRDPFIIMLTVPLSMTGALGALWLTGGTLNVYSQIGLVTLVG
                     LITKHGILIVEFANQLREHGHALREAVVEAAVLRLRPILMTTGAMVLGSLPLALATGA
                     GAESRQQIGWVIVGGLLIGTFFTLFVVPTVYTLLARREREAPVAVAQGAAS"
     gene            complement(5883..6104)
                     /locus_tag="PKV97_08795"
     CDS             complement(5883..6104)
                     /locus_tag="PKV97_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HNB06012.1"
                     /translation="MFPEYRDLITRLKHNDRHFTQLFERHNELDQKIHNIETHVVPGT
                     SLEIEVMKKEKLAIKDQIYAYLKRVETES"
     gene            complement(6319..6864)
                     /locus_tag="PKV97_08800"
     CDS             complement(6319..6864)
                     /locus_tag="PKV97_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004378041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HNB06013.1"
                     /translation="MDEQIIADVEQWLDEVVIGLDLCPFAARPRREKRVRIAVSHAST
                     ADALLEDLQAELERLSDTPVAELETTLLAIPDMLEDFADYNDFLDAVDLWVEQFGWEG
                     ELQVASFHPQYQFADTEADDPGNLTNRSPWPLLHIIREESLEKAIEHYPEVDRIPERN
                     IARMNALSGEERAKLFPHLFG"
     gene            complement(6879..>7416)
                     /locus_tag="PKV97_08805"
     CDS             complement(6879..>7416)
                     /locus_tag="PKV97_08805"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HNB06014.1"
                     /translation="ARLDPEAAARLEPGDAQRIQRALEIVRLTGRPLADSYAKKEDDP
                     LPCRLLPIALAPSDRGALHARIEERFDAMLHAGLVDEVRQLRARYRLDPSMPSMRCVG
                     YRQVWEYLDGGTGYDELRFKGVAATRQLAKRQLTWQRQFRETWPGFVELDCLRPDLPE
                     AVLHTALRLLHDLPLHPA"
BASE COUNT          991 a         2445 c         2796 g         1184 t
ORIGIN      
        1 gccgctggcg agacttcgtg cagtgccgtg ttgcattcga ggcagcgcga gaacgggcgg
       61 gcgtgtccgg cgagtccgag gcggcggagg atctcgaccg cctggcgcgc cggctcgagc
      121 gcatgcacga agcagccctc ggcgacgtcc cggcgcttga gcagttcgcg gtcgcgggtc
      181 agcacgacgc gccgctcgcg cacggcggtg gcgacgagcg tggcgtcctc gatgtgattg
      241 tcgtagagcg tgtcgaagcc ggccaggcgc aggagtcgcg ccaggccgcc gagatgggcg
      301 tcggcgatga ggcgcggcgg cggggacggg cagggcttcc cgcagcgcgc ggcatgggga
      361 tggaccgaca cccgctcgcc cgggcacaag cggcgctcga ggggcgccgg gcgaccgtcg
      421 accagcaccc ttccgacctc ggtgtgcggg acgccgagcg cttcgatcac gtgcttgagg
      481 ctggagtcgg ccgcgcagcg gacctcgaag gtgcggtcgc gccgtgccgg ggcgaggaag
      541 gccgccaact cgccatggaa gcggaaggag acgagccgtg cgggggagag gggcacgggg
      601 gtggcgtcgg acatgggcag ggcattccct cgatcgagca ccgccacagc atagcgcgcg
      661 ccgcaggcgc ttgctgctac gctgtcggct ggttcgtccg aggagatcga agatgaagat
      721 ccgcgccctg ttcgtcacgc tttccgggct ctctgccgcc gctgtggccg tgctcctcct
      781 cgccgcggtc ccggcacccg ccggcgcgat cgcccagccg gtgggcccgg cgatgccgga
      841 ggaatgcgtc tgttcgcgcg gcctgcgcct cgcggaggcg ccgccctcgg tcctgcacaa
      901 ctgccagtgc ggtccgctgc agtgcgtggt gcacgcgcag tccggccagc tgcagtgccg
      961 ctgagccgcg tgtgcgcatg aagccgcgcc tcagcgtgat cacgctcgcg gtggacgatc
     1021 tcgagcgcgc cgtcgccttc tatcgcgacg gcttgggctg gccgacgcag ggcatcgtgg
     1081 ggagtgcggc gcagcattcg gcggtcgcct tcttcgcgct gcagcccggg atccggctgg
     1141 cgctcttccc gcgcacgagt ctcgcggtcg aggccggcgt cgcggaaccg ccgtcgggca
     1201 cgagcggctg cctgctgagc cacaacgtcg cccggcgcga gcaggtcgcc gaggtgatgg
     1261 ccgaggccgc ggtggcgggt gcgcgcattc tgcgtccgcc acgcgacacc gcctggggtg
     1321 gccatgcggg ctatttcgcg gatctcgacg ggcatccgtg ggaggtggcg tggaacccga
     1381 aatggccgct ggcggacgac tgagtcccgc gcaggctggc tgagcggatg cagcccggcg
     1441 caacgcgacc gtcccgcggc gcggaaccgg acagcgcgcg cctttgtccc taaactggcg
     1501 gctcgcgccg agccacgctc tccactcctc acgcccgact cctcgtttcc cgatgccttc
     1561 cagccgccgc ttcgtcctct cgctcagcct cgtcggtctc gccgcgctgg gcgcctatgc
     1621 ctggtatgca aaccgtccgc cggccttgcc gccggggggc gcgcgggcgc aggcgcaggc
     1681 ggcaggcgtg caagcggtgc cgcctgcggc ggggactgcg ggtgcgaagg gggccgcggg
     1741 cgcgaagaag ggggccgcgg gcgctgcggg tggctcgcag ggcgggccgg tggtggtgga
     1801 gacgcacacc gtgggcgtgc gtgcgctcgc cgacgacgtc ggcgcggtcg gctcgctggt
     1861 gtcgaacgag tcggtggtgc tgcgccccga ggtgtcgggg cggatcgagg cgatccgctt
     1921 tcgcgatggc gaggcggtgc ggcgcggcga ggtgctgatc gagttcgacg ccgcggtgca
     1981 gcgcgccgag ctgcagcagg cgcgcgccag cctgaccttg gccgaatcga acttccgccg
     2041 cagccaggac ctcttttcgc gcagcttcgt gagccgcagc agcctggaca acgcgcgctc
     2101 gcagctcgag gtggcgcgcg ccggggtggc gctcgcgcag gccaggctcg cgcgcatgca
     2161 gatccgcgcg ccctttgccg gcgtcgtcgg catccgcagc gtgagccccg gcgacttcgt
     2221 caaggacggc gaggccatga tcaacctcga ggacatcgcc acgctgaagg tggatttccg
     2281 cctgcccgag ctctacctcg accgcgtgcg ccccgggcag acggtggaga tcgcgagcga
     2341 cgtgctggcg ggcgagcgtt tcgccgccac ggtggaggcg atcgatccgc tggtcgatgc
     2401 gcaggggcgc gcgctcaggc tgcgcgcgag cctgcccaac cccgagctgc gcctgcgccc
     2461 gggcgtgttc gtacgcgtgc gcctgatcct cgccgagcgc gccgacgtcg tggtggtgcc
     2521 cgaggccgcg ctggtgccgg cgccgggcaa cacccagttc gtataccggg tcgccgaggg
     2581 caaggcgcag cgcgtcgagg tgcgcaccgg cacgcggcgc gacgcgatgg tggaggtggt
     2641 cgagggcgtg caggcgggcg aggtggtggt gaccgccggc cagctcaagc tgcgcgacgg
     2701 cgcggcggtg aagctggcca cgggcggcgt ggcgcgggcg gactgagcga tggtcctctc
     2761 cgagctctgc atccgccgcc cggtgttcgc caccgtgctg tcgctgctcg tgctgctggt
     2821 gggcctgatg tcgtactcgc ggctcaccgt gcgcgagtac cccaacatcg acgagccggt
     2881 cgtgacggtg gatacgcgct accgtggcgc cagcgccgag atcgtcgagt cgcaggtcac
     2941 caagccgctg gaggattcgc tcgccggcat cgagggcgtg gacgtgctgt cctcgatcag
     3001 ccgccaggag cgcagccaga tcacggtgcg cttccgcgtc gagcgcagcg cggattcggc
     3061 tgcggccgac gtgcgcgacc gcgtgtcgcg cgtgcgccag cgcctgcccg acgacatcga
     3121 cgagccggtg atcgccaagg tcgaggccga cgccagcccg atcatctggg tggctttttc
     3181 ctccgaccgc cacgcgccga tggagatcag cgacttcgcc aaccgcgtca tcaagccgcg
     3241 tctgcagacc ttgcccggtg cggccgatgc gcgcctgttc ggcgaccgcc gctcggcgat
     3301 gcgggtgtgg ctcgatcgcg accgcctcgc cgccttcggc ctgaccgtgc aggaggtgga
     3361 agacgcgatc cggcgccaga acgtggaact gcccgccggg cgcatcgaga gccgcgagcg
     3421 cgagttcgcc gtcgtggcgc agaccgacct cgcccggccc gagcagttcg gcgccatcgt
     3481 gctgcgccag ggcgacaccg ccgacgccta cccggtgcgc atccgcgacg tcggccgggt
     3541 ggaggtggca cccgagagcg agcgcacctc ggtgcgcttc atggggcggc cggcggtgtc
     3601 gatcggcctc atcaagcaat cggtggcgaa cccgctcgac ctcaagcgtg gcctggacga
     3661 gatgatgccg cggctgcagg ccgagctgcc cgaaggcatg tcgatgacca tcgccaacga
     3721 caccacggtg ttcatcgagc gctcgatcga cgcggtgttc tccaccatct gggaggcggt
     3781 gctgctggtg gccgcggtgt gcctgttctt cctgcgcaac tggcgggcga tgctggtgcc
     3841 gctggtgacg atcccggtgt cgctggtggg cgcgttcacg ctgatgctgg tgctcggctt
     3901 ctcgatcaac acgctcacgc tgctcgcgct ggtgctggcg atcgggctgg tggtggacga
     3961 tgcgatcgtg gtgctggaga acatctaccg ccacatcgag gacggcatgg agccggtcgc
     4021 cgccgccttc aagggcgcca gggagatcgg cttcgcggtg gtggcgatga cgatcacgct
     4081 ggccgcggtg tacgcgccgg tggccttcat gagcgggcgc acgggcaagc tcttcaccga
     4141 gttcgcgctc acgctcgccg gcgcggtgat cgtgtcgggc ttcgttgcgc tgacgctgtc
     4201 gccgatgatg tgctcgaagc tgctgcgtca cgagcccacg cacggacgga tctacaacgg
     4261 catcgagcgt ttcctggcct ggctcaccgg cggttatcgc gctgtgctcg agctggcgct
     4321 gggcgcgcgc tggctggtga tgctggtgtt cgcggtggtg gcggggagct cggtctggct
     4381 gatggggcag ttgaagagcg agctcgcgcc gctggaggac cgcggtcggg tgatcggcat
     4441 gttcaccggc ccggagggcg ccacgctcga ctacatggcg cgttacgccg aggagatcga
     4501 gcgcatctac gccggcctgc cggaagtcga tcgctatctg gtgatcgccg gcaacccgac
     4561 ggtgtcgcag ggcatctcct tcgtcggctt caccgactgg tcggagcgta cgcgtacggc
     4621 cggggagatc tcgagcggac tgcagcccaa gctgcgcgcc gtgccgggcg tgaatgcctt
     4681 cccgatcatg ccggcttcgt tcgggcagag tgcgcgctcg cggccggtga gcttcgtggt
     4741 ggcgacctcg gagagctacg acgcgctcgc gcgcgtgacc gacaccttcc tcgacgcgat
     4801 gcgcgacaac cccggcttcc tgtcgccgga caccgacctc aagctgacga tgcccgagct
     4861 tgcggtggag gtggatcgcg accgcgcggc cgacctcggg gtcgacgtcg atgtgatcgc
     4921 gcgcacgctc gagagcatgc tcggcggtcg ccaggtcacg cgctacaagc aggacgccga
     4981 gcagtacgac gtgatcgtgc aggtcggcgc cggcgagcgc cgcgacccgc gcgacatccg
     5041 cgacatctac gtgcgtgcgc gcaccggcga gatggtgccg ctgaccagcg tggtgcaggt
     5101 gagcgagcgc gtggcgccgc gcgaactcaa ccacttcggc cagcgccgct cggtgaccat
     5161 ctcggccaac ctggcgcccg attacgccct gggcgaggcg ctgcagtgga tggacgacac
     5221 cgcggcgcgc atcctgccgc cgggctacag caccgactac gacggccagt cgcgcgagtt
     5281 caagaccagc gcggagagcc tcgcgctggt cttcctgctc gcgctcgcct tcatctacct
     5341 ggtgctggca gcgcagttcg agagcttccg cgaccccttc atcatcatgc tgacggtgcc
     5401 gctgtcgatg accggcgcgc tcggcgcgct atggctgacc ggcggcacgc tcaacgtcta
     5461 cagccagatc ggcctggtga cgctggtcgg gctgatcacc aagcacggca tcctgatcgt
     5521 ggagttcgcc aaccagctgc gcgagcacgg acatgcgctg cgcgaggcgg tggtggaggc
     5581 ggcggtgctg cgcctgcggc cgatcctgat gacgaccggg gcgatggtgc tgggctcgct
     5641 gccgcttgcg ctggcgaccg gggcgggcgc cgagagccgc cagcagatcg gctgggtgat
     5701 cgtggggggc ttgctgatcg gcaccttctt cacgctcttc gtggtgccga cggtgtatac
     5761 gctgctggcg cggcgcgagc gcgaagcgcc ggtcgcggtc gcgcagggag ccgcctcctg
     5821 aaaacgaaga accccgccgc gccggtcgcg cttgcggggt tcgtcacggt gccgggaggg
     5881 gatcagcttt cggtttcgac cctcttcagg tacgcgtaga tctggtcctt gatcgccagc
     5941 ttctccttct tcatgacctc gatctcgaga ctggtgccgg gcacgacgtg ggtctcgatg
     6001 ttgtggatct tctggtcgag ctcgttgtgc ctctcgaaga gctgggtgaa gtggcggtcg
     6061 ttgtgcttga gacgggtgat caggtcgcga tactcgggga acatgggaac aactccttgg
     6121 ttctggattg caaaggctac gaactggggc aaaggaaaaa ctttccgctt ggcaccagtc
     6181 tactcccgct tccggtgcgc ggccagcgtg gcgggcgggt gtggcccgtg gtccgcgacc
     6241 cgcttcgggc cgcttgcgga tggcatttgc cgggctgttg cggcgagcag gaaaactgat
     6301 cgaaagttcc tcggtgcctc atccgaacag gtgggggaac agcttcgcgc gctcctcgcc
     6361 actgagtgcg ttcatgcgcg cgatgttgcg ctcggggatg cggtcgacct cggggtaatg
     6421 ctcgatcgcc ttctccaggc tctcctcgcg gatgatgtgc agcagcggcc agggcgagcg
     6481 gttggtcagg ttgcccgggt cgtccgcctc ggtgtcggcg aactggtact gcgggtggaa
     6541 gctcgccacc tgcagctcgc cctcccagcc gaactgctcg acccacaggt ccaccgcgtc
     6601 gaggaagtcg ttgtagtcgg cgaagtcctc cagcatgtcg gggatcgcca gcagggtggt
     6661 ctcgagctcc gccaccgggg tgtcggagag gcgctcgagc tcggcctgca ggtcttcgag
     6721 cagggcgtcg gcggtgctgg cgtggctgac cgcgatgcgc acgcgcttct cgcgccgcgg
     6781 gcgggcggcg aaggggcaga ggtcgaggcc gatgacgacc tcgtcgagcc actgctcgac
     6841 gtcggcgatg atctgttcat ccattggagc ggttccgttc aggcgggatg caggggaagg
     6901 tcgtgcagca gccgcaaggc ggtgtgcagc acggcctcgg ggaggtcggg gcgcaggcag
     6961 tcgagctcga cgaagcccgg ccaggtctcg cggaactggc gctgccaggt gagctggcgc
     7021 ttggcgagct ggcgggtggc ggcgacgccc ttgaagcgca gctcgtcgta gccggtgccg
     7081 ccgtcgaggt attcccacac ctggcggtag ccgacgcagc gcatcgaggg catggacggg
     7141 tcgagccggt agcgtgcgcg cagctggcga acctcgtcga ccaggccggc gtggagcatg
     7201 gcgtcgaagc gctcctcgat gcgcgcgtgc agcgcgccgc gatccgaggg cgcgagcgcg
     7261 atcggcagca gccggcaagg cagcgggtcg tcttccttct tcgcgtagct gtccgcgagc
     7321 gggcggccgg taaggcggac gatctcgagc gcgcgctgga tgcgctgcgc gtcgcccggc
     7381 tccaggcgtg ccgcggcctc ggggtcgagg cgggcg
//
LOCUS       DAOQNN010000137         7412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5248_length_7412_cov_10.707218, whole genome shotgun sequence.
ACCESSION   DAOQNN010000137 DAOQNN010000000
VERSION     DAOQNN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5248_length_7412_cov_10.707218"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..2520)
                     /locus_tag="PKV97_08810"
     CDS             complement(127..2520)
                     /locus_tag="PKV97_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB06015.1"
                     /translation="MLNPFSVSEVDTFLTRYLRLLEAVCFRFRGTILVALMLFTALMG
                     YFALQLRMDAGFEKQMPIGHEYIQTFDKYRDDVLGANRLNFVVKARSGDIWTQAGLAR
                     LYEVTQAVSFLPNVDRLGVQSLWTPNSFVNEITEEGFRADPLISGTIMPRDLTPEIIA
                     GIQRAASQGGFIGTLVARDQTSAMIVAELHERDREGKALDYVAYNRILEELRAKFEDA
                     QFEVQIIGFAKQIGDIADGASAVLEFCAIALLLTALAVYWYCHSVRFTLLPIACSLTS
                     LVWQFGTLRLLGYGLDPLAVLVPFLVFAIGVSHGVQQINFIVRELSHGKSSDEAARAS
                     FTGLLIPGTLALVTAFVSFITLILIPIPMVRELAITASLGVGYKIVTNLVMLPLAASL
                     FTFTKEYADKAVLKREQRSAWLRTLARVAEPRNAAIVIVATLAVFAVAVWQSHDRVIG
                     TVQPGAPELRAEARFNRDAVSIAASYDTGLDWLTVIFEAPPDACDDVAVGRYQDRFVA
                     EIQYVPGVLSALSFSGQLRTYNEGYNEGNPKMSVVPGDPGNYAALATEIGRVRGFMNK
                     DCSMTAVHLFLSDHKATTINRVIEAVKDFRERDREPGLTIRLASGNAGVQAAVNEEVH
                     KSELPMMLYVYAAIMVLVALMYRDFRAVVACCLPLTVGTFIGYWFMKELEIGLTVATL
                     PVMVLAVGIGVDYAYYIYNRLQLHLARGENIVKALEHSILEVGTATIFTAITLAAGVA
                     TWMFSELKFQADMGKLLAFMFMVNMVMAMTALPAFAVWLERLFPRKGPVRAPGILAH"
     gene            complement(2596..4323)
                     /locus_tag="PKV97_08815"
     CDS             complement(2596..4323)
                     /locus_tag="PKV97_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HNB06016.1"
                     /translation="MTTHLKKLVLGLAMAGICAPAAYAFQFEAGSVRGSLDSTLTAGF
                     GKRVEGRECSHHGDQSTPCGADANTAVWGNGDDGNLNYDKGDLFTTHLKGSHELLLSF
                     PDEWKFMGRVGWLYDFVADDTARTPLDGDAKQAVGQYVRLYDLWVSKEFDLGERRGRV
                     RFGNQVVSWGESLFMIGGINSNVAMDLQRLSSPGVQLKEAFLPSPALSVAANLGSGVN
                     VEAYYQFQWKPYSFPPAGTYFSQSDFFDKGRDSLIYFTPDAASRIRTPGDPLYGKTLA
                     AAREEMLNSELTAIPVDADDEPGDSGQFGVSLKYRPEGMDVDLGLYYQRFHDKTPNLQ
                     YWNANGAARMYFLEDRELYGISANTSVGNWAVGAELSYRPEDAVSLGACQDIGFGSPD
                     QCDGSIDEERYQFHLTGILSLTPGDHGWFLDLMGAQTGTFLGEAVAIAYPGVSKDKAY
                     VRTRNGVTYQQLPAAGAWTHDSGVGDKLSWGYMFDFSLTYDSTLIPGWQVIPGVFFSH
                     AVNGNTPNFMANWMEGAKSANFYVLFNRNPMTWQAGINYTRFWGGDYSFSAPYKDRDF
                     VGGFISRNF"
     gene            complement(4416..5783)
                     /locus_tag="PKV97_08820"
     CDS             complement(4416..5783)
                     /locus_tag="PKV97_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HNB06017.1"
                     /translation="MRFCKLTALAGALLASHAALAADAASLGTTLTPLGGERAGNADG
                     SIPAWTEGSALEPGWSYGKPRADYFKHKGDKPLFTIDAANVDKYAGKLTEGQVAVIKG
                     FKNYKLEVYPSRRYCSAPDFVQANTKANVGMAKIGADGWSLAEATVPGIPFPLPQSGI
                     EVLWNAKMKYAGVALDMKALWVMLSPRSGGSDWIEAGSTQTYYYPWGKKGSNKLSELP
                     PVEYHTYFNYTSPTALAGQALVITSYLNKTSDTFYYFPGQRRVRRMPSYSYDAPQVGF
                     ENQYTLDEPRVFNGTPDRFDWKLVGKKEMFIGYGSFGMYDPAADRRKVVTPDGVDPKA
                     TRYELHRVWVVEATAKDGVRHVAPKRRFYFDEDSWALMGAEDYDAQGKLWKVRESFLI
                     PVAETGACDNPAFVQYDLVSGRVLFDQAGMGAGKDMVWAVEADDPKYKDAFYTPDNLR
                     AISDR"
     gene            complement(6011..6535)
                     /locus_tag="PKV97_08825"
     CDS             complement(6011..6535)
                     /locus_tag="PKV97_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HNB06018.1"
                     /translation="MKARSVRDMTGPRGPRRIRMNGMKAVACTALGGLVLAISANAGA
                     TGPDQAFDDMLAARYRAVEAAIDARDGAAWFEALYDDSIVLAGEGSPATVRGRQALMP
                     VIDEIVRTTRRCTLVPDAARDRSAGLAYSFVTYRCSPADASASDYQVRALFVWKEGPK
                     GWRVVAESYVMGAM"
     gene            complement(6535..6909)
                     /locus_tag="PKV97_08830"
     CDS             complement(6535..6909)
                     /locus_tag="PKV97_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB06019.1"
                     /translation="MSRSFSLSAVVAILSVLGLPAPALATDNGESGFVQCAACHPAAA
                     GQPHGVGPNLHGRFGQPIAGTPDFVFSRALRKLQGVWSEAELDRWLSDPQAYAPGTKM
                     AYAGMKDPAQRRAVIEYLKALR"
     gene            complement(6924..>7412)
                     /locus_tag="PKV97_08835"
     CDS             complement(6924..>7412)
                     /locus_tag="PKV97_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB06020.1"
                     /translation="GKTAMAKEHHAGKGHKLYLKVKGRLETLIFFAPETELFTMVFTD
                     QPGEDGGVLVAFGPPVDGRVDLNDPKAVEPFVRRFLPHLAVEQVLGYDWGLDPYSRGT
                     WCTLRPGQFGKHLEDLRAREGRVFFAGADIATGWRGFIDGAIESGLQVGQAVAAELRG
                     RA"
BASE COUNT         1196 a         2476 c         2542 g         1198 t
ORIGIN      
        1 tgcaccgacg tatcgccggc agcgggcgcc aggctcgcgc agagcgccgc gcagcaggcc
       61 gcgatgcggg tcgcgaagaa gcgcacgcgg cgcggcgtcg agaaggcgtt catgtccact
      121 ccgcagtcag tgcgccagga tgcccggcgc gcgcaccggc cccttgcgcg ggaaaaggcg
      181 ttccagccac accgcgaagg ccggcagcgc agtcatcgcc atcaccatgt tcaccatgaa
      241 catgaaggcg agcagcttgc ccatgtcggc ctggaacttg agctccgaga acatccaggt
      301 cgccacgccc gccgcgagcg tgatcgcggt gaagatggtc gcggtgccga cctcgaggat
      361 ggagtgttcc agcgccttga cgatgttctc gccgcgggcg aggtgaagct gcaggcggtt
      421 gtagatgtag taggcgtaat cgacgccgat gccgaccgcc agcaccatca ccggcagggt
      481 cgccaccgta aggccgatct cgagctcctt catgaaccag tagccgatga aggtgcccac
      541 ggtgagcggc aggcagcacg ccaccacggc acggaagtcg cggtacatca gcgccaccag
      601 caccatgatc gccgcgtaca catagagcat catcggcagc tcggacttgt gcacctcctc
      661 gttcaccgcc gcctgcacgc cggcgttgcc cgaggccagc cggatggtca gcccgggctc
      721 gcggtcgcgc tcgcggaagt ccttcaccgc ttcgatcacg cggttgatcg tggtcgcctt
      781 gtggtcggac aggaacaggt gcaccgcggt catgctgcag tccttgttca tgaagccgcg
      841 cacgcggccg atctcggtgg cgagcgcggc gtagttcccg gggtcgcccg gtaccaccga
      901 catcttcggg ttgccctcgt tgtagccctc gttgtaggtg cgcagctggc cggagaagga
      961 cagcgccgac agcacgcccg gcacatactg aatctcggcg acgaagcggt cctgatagcg
     1021 cccgaccgcg acgtcgtcgc aggcgtcggg cggcgcctcg aagatgaccg tgagccagtc
     1081 caggccggtg tcgtagctgg cggcgatcga caccgcgtcg cggttgaagc gcgcctccgc
     1141 acgcagctcc ggggcgcccg gctgcaccgt gccgatgacg cggtcgtggc tctgccacac
     1201 cgccaccgcg aacaccgcca gggtcgccac gatcacgatc gccgcgttgc gcggctcggc
     1261 cacgcgcgcc agcgtgcgca gccaggccga gcgctgctcg cgcttgagca cggccttgtc
     1321 ggcgtattcc ttggtgaagg tgaacagcga ggccgccagc ggcagcatca ccaggttggt
     1381 gacgatcttg tagccgacgc cgagcgaggc ggtgatcgcc agctcgcgca ccatcgggat
     1441 cgggatcagg atcagcgtga tgaaggacac gaaggcggtg accagcgcca gcgtgcccgg
     1501 gatcagcagc ccggtgaagc tggcgcgcgc ggcctcgtcg ctcgatttgc cgtgcgagag
     1561 ctcgcgcacg atgaagttga tctgctgcac gccgtgcgag acgccgatcg cgaacaccag
     1621 gaagggcacc agcaccgcga gcgggtcgag cccgtagccg agcagcctga gggtgccgaa
     1681 ctgccacacc agcgaggtca gcgagcaggc gatcggcagc agggtgaagc gcaccgagtg
     1741 gcagtaccag tacaccgcga gcgcggtcag cagcagcgcg atcgcgcaga actcgagcac
     1801 cgccgaggcg ccgtcggcga tgtcgccgat ctgcttggcg aagccgatga tctggacctc
     1861 gaactgcgcg tcctcgaatt tcgcgcgcag ctcctcgagg atgcggttgt aggcgacgta
     1921 gtcgagcgcc ttgccctcgc ggtcgcgctc gtgcagctcg gcgacgatca tcgcgctggt
     1981 ctggtcgcgc gccaccaggg tgccgatgaa gccgccctgg ctggccgcgc gctggatgcc
     2041 ggcgatgatc tcgggcgtga ggtcgcgcgg catgatggtg cccgagatca gcggatcggc
     2101 gcgaaagccc tcctcggtga tctcgttgac gaaggagttg ggcgtccaca gcgactgcac
     2161 gccgaggcgg tcgacgttgg gcaggaagct caccgcctgg gtgacctcgt acagacgcgc
     2221 cagcccggcc tgcgtccaga tgtcgccgct gcgcgccttg acgacgaagt tcagtcggtt
     2281 ggcgccgagg acgtcgtcgc gatacttgtc gaaggtctgg atgtactcgt ggccgatcgg
     2341 catctgcttc tcgaagccgg cgtccatgcg cagctgcagg gcaaagtagc ccatcagcgc
     2401 ggtgaacaac atgagcgcga ccaggatggt gccgcggaag cggaagcaca ccgcctcgag
     2461 caggcgcagg tagcgggtca ggaaggtgtc gacctcgctg acgctgaagg gattgagcat
     2521 gggcgggtgc tggcgatcgg agtgggaacg gggcagcggg ccggaggcgg cgctcgccgc
     2581 ccccgggggt gctcctcaga agttgcgcga gatgaagccg ccgacgaagt cgcgatcctt
     2641 gtagggcgcg ctgaagctgt agtcgccgcc ccagaaccgg gtgtagttga tgccggcctg
     2701 ccaggtcatc gggttgcggt tgaacagcac atagaagttg gccgacttcg ccccctccat
     2761 ccagttggcc atgaagttgg gcgtgttgcc attgaccgca tgcgagaaga acacccccgg
     2821 gatcacctgc cagcccggga tcagggtgct gtcgtaggtc aggctgaagt cgaacatgta
     2881 gccccaggac agcttgtcgc cgacgccgct gtcgtgcgtc cacgcgccgg cggcggggag
     2941 ttgctggtag gtcacgccgt tgcgggtgcg cacgtaggcc ttgtccttgc tcacccccgg
     3001 ataggcgatc gccaccgcct cgccaaggaa ggtgccggtc tgcgcgccca tcaggtcgag
     3061 gaaccagccg tggtcgccgg gcgtcaggct gaggatgccg gtgaggtgga actggtagcg
     3121 ctcctcgtcg atcgagccgt cgcactgatc gggtgacccg aagccgatgt cctggcaggc
     3181 cccgagcgaa accgcatcct ccggccgata cgacaactcg gcgcccaccg cccagttgcc
     3241 caccgaggtg ttggcgctga tgccgtagag ctcgcggtcc tccagaaagt acatgcgagc
     3301 cgcaccgttc gcgttccagt attgaaggtt gggggtcttg tcgtggaagc gctggtagta
     3361 gaggccgaga tcgacgtcca tgccctcggg ccggtacttc agcgagacgc cgaactggcc
     3421 ggagtcgccc ggctcgtcgt cggcgtcgac cgggatcgcc gtgagttcac tgttcagcat
     3481 ttcttcccga gccgcggcga gggtcttgcc gtaaagcgga tcacccggcg tacgaatccg
     3541 gctggcggca tccggcgtga aatagatcag gctgtcacgc cccttgtcga agaaatcgct
     3601 ctgcgagaaa tacgtccctg ccggcgggaa gctgtagggc ttccactgga actggtagta
     3661 ggcctcgacg ttgactccgc tgcccaggtt ggcggcgacc gacagcgccg gcgagggcag
     3721 gaaggcctcc ttcagctgca cgccgggcga ggacaggcgc tgcaggtcca tcgccacgtt
     3781 ggagttgatg ccgccgatca tgaacaggct ctcgccccag ctcaccacct ggttgccgaa
     3841 gcgcacgcgg ccgcggcgct cgccgaggtc gaattccttg ctcacccaca ggtcgtagag
     3901 gcgcacgtac tggccgaccg cctgcttggc gtcgccgtcg agcggggtgc gcgcggtgtc
     3961 gtcggcgacg aagtcgtaca gccagcccac ccggcccatg aacttccact cgtcgggaaa
     4021 gctcagcagc agctcgtgcg agcccttgag gtgggtggtg aacaggtcgc ccttgtcgta
     4081 gttgagattg ccgtcgtcgc cgttgcccca caccgcggtg ttggcatcgg cgccgcaggg
     4141 ggtggattga tcgccgtggt ggctgcactc gcggccctcc acgcgcttgc cgaagcccgc
     4201 ggtcagcgtg gaatcgagcg aaccgcgcac cgagccggcc tcgaactgga aggcgtacgc
     4261 cgccggcgca cagatgccgg ccatggccag ccccagcacc agtttcttca gatgtgtcgt
     4321 catgatcgtt ctccgcagga cgccccgccc gggccgcatg acggcccggc gcacgcgccc
     4381 gggtgatggt cgggaagaaa gaggagggag cgggctcagc ggtcgctgat cgcgcgcagg
     4441 ttgtccgggg tgtagaaggc gtccttgtac ttcggatcgt cggcctcgac cgcccagacc
     4501 atgtccttgc cggcacccat gcccgcctgg tcgaagagca cacggccgga gacgaggtcg
     4561 tactgcacga aggccgggtt gtcgcaggcg cccgtttcgg cgaccggaat caggaagctc
     4621 tcgcggacct tccacagctt gccctgggcg tcgtagtcct ccgcgcccat cagcgcccag
     4681 gagtcctcgt cgaagtagaa gcggcgcttg ggcgcgacgt ggcgcacgcc gtccttggcg
     4741 gtggcctcga ccacccagac gcggtgcagc tcgtagcggg tcgccttcgg atccacgccg
     4801 tcgggcgtca ccaccttgcg gcggtcggca gcggggtcgt acatgccgaa gctgccgtag
     4861 ccgatgaaca tttccttctt gcccaccagc ttccagtcga agcggtcggg cgtgccgttg
     4921 aagacgcgcg gctcatcgag cgtgtactgg ttctcgaagc cgacctgggg ggcgtcgtag
     4981 gagtagctcg gcatgcggcg cacgcggcgc tggccgggga agtagtagaa ggtgtcgctg
     5041 gttttgttca ggtacgaggt gatcaccagc gcctggccgg ccagtgcggt gggcgaggtg
     5101 tagttgaagt aggtgtggta ctcgaccggc ggcagctcgc tgagcttgtt cgagcctttc
     5161 ttgccccagg ggtagtaata ggtctgcgtg gacccggcct cgatccagtc gctgccgccg
     5221 ctgcgcggcg acagcatcac ccacagcgcc ttcatgtcga gggcgacgcc ggcgtacttc
     5281 atcttggcgt tccacagcac ctcgatgccg ctctgcggca gcgggaaggg aatgccgggc
     5341 acggtggcct cggccaggct ccagccgtcg gcgccgatct tcgccatgcc tacgttggcc
     5401 ttggtgttgg cctgcacgaa gtcgggcgcg gagcagtagc ggcggctcgg atagacctcg
     5461 agcttgtagt tcttgaagcc cttgatcacc gcgacctggc cctcggtgag cttgccggcg
     5521 tatttgtcga cgttggcggc atcgatcgta aacagcggct tgtcgccctt gtgcttgaag
     5581 tagtcggcgc gcggcttgcc gtagctccag cccggctcga gtgccgagcc ttccgtccag
     5641 gccgggatgc tgccgtcggc gttgcccgcg cgttcgccgc cgagcggggt cagcgtggtg
     5701 cccaggctcg ccgcgtcggc ggccagggcg gcgtggctgg ccagcagggc gccggcgagc
     5761 gcagtcagct tgcagaacct catgagtgtc tcctccacgt ttctgatttt gtcggcagcg
     5821 gcaggcggtc accgaggacg ccaccgctgc gcagcggccg accgggcccg ccttcccgcc
     5881 agccttgcag gcctatggtg tgtcgagcat agcgacgcgg tgtaaaccgc atttccgcga
     5941 aatgtgccga tttgtgcgca gttgtagcgg catcccccac ggcccggcgg gccgggggcg
     6001 ctgccgccac ctacatcgcg cccatcacat agctctcggc aaccacccgc cagcccttgg
     6061 ggccctcctt ccagacgaag agcgcgcgca cctggtagtc cgacgccgac gcgtcggcgg
     6121 gcgaacaccg ataggtcacg aacgagtagg caaggcccgc cgagcggtcg cgcgccgcat
     6181 ccgggaccag ggtgcagcgg cgcgtggtac gcacgatctc gtcgatcacc ggcatcagcg
     6241 cctgccgccc gcgcaccgtg gcggggctac cctcgcctgc cagcacgatc gagtcgtcgt
     6301 acagggcttc gaaccatgcc gcgccatccc gtgcatcgat cgccgcctcc accgcgcgat
     6361 agcgcgccgc gagcatgtcg tcgaacgcct gatccggtcc cgtggccccc gcgttcgcgg
     6421 agattgcgag cactagtccg ccgagtgccg tgcatgcaac agccttcatc ccattcattc
     6481 gaatcctcct tggcccgcgc gggccggtca tgtcccgaac gctgcgcgcc ttcatcagcg
     6541 cagcgccttc agatactcga tcaccgcccg gcgctgggcc gggtccttca tgcctgcata
     6601 ggccatcttg gtcccgggcg cgtaggcctg cggatcggag agccaccgat ccagctccgc
     6661 ctccgaccac accccctgaa gcttccggag cgcacgggaa aagacgaagt ccggggtgcc
     6721 cgcgatcggc tgcccgaagc ggccgtgcag gttgggtccc acgccgtggg gctgccctgc
     6781 agcggcggga tggcaggcgg cacattggac gaagccggac tcgccgttgt cggtggcaag
     6841 cgccggagcc gggaggccga gcacgctcag gatcgcgacc acggccgaca gcgaaaagga
     6901 tctgctcatc gcaccgccct ccatcacgcc cgaccgcgca gctcggccgc gaccgcctgt
     6961 ccgacctgca ggccggactc gatcgcgccg tcgatgaagc cgcgccagcc ggtggcgatg
     7021 tccgccccgg cgaagaacac ccggccttcg cgggcacgca ggtcctccag gtgcttgccg
     7081 aactgccctg gacgcagcgt gcaccaggtg ccgcgcgaat acggatccag cccccagtcg
     7141 tagccgagca cctgttcgac cgcaaggtgg ggcaggaagc ggcgcacgaa gggctcgacc
     7201 gccttcgggt cgttgagatc gaccctgccg tcgactggcg gaccgaaggc gaccagcacg
     7261 ccgccgtcct cgcccggctg gtcggtgaac accatcgtga acagctcggt ctcgggcgcg
     7321 aagaagatca gcgtctccag ccggcccttc accttcagat agagcttgtg ccccttgccc
     7381 gcgtggtgct ccttcgccat cgcggtcttg cc
//
LOCUS       DAOQNN010000138         7368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5296_length_7368_cov_10.871325, whole genome shotgun sequence.
ACCESSION   DAOQNN010000138 DAOQNN010000000
VERSION     DAOQNN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5296_length_7368_cov_10.871325"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..677
                     /gene="lolA"
                     /locus_tag="PKV97_08840"
     CDS             18..677
                     /gene="lolA"
                     /locus_tag="PKV97_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HNB06021.1"
                     /translation="MIQFNTRPAALLRGVAGVVLALAALGATAAGGVQQLRDFVAGAR
                     SAEGEFEQVVTAQSGRRPQEASGSFAYARPGRFHWEYDRPYRQVLVGDGKTLWSWDPD
                     LNQVTVRAIGEALGATPAAILFGSGALDDNFELADAGERDGLAWAEARPKRTESGFES
                     LRIGLAEGQLRRMEMRDHFGQTTVIRFTRLRANPVLAEDRFRFEPPAGADVIGELPGA
                     R"
     gene            748..2172
                     /locus_tag="PKV97_08845"
     CDS             748..2172
                     /locus_tag="PKV97_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNB06022.1"
                     /translation="MTDLFDALEPPQVPLAERMRPHTLDEVAGQSHLLGPGKPLRLAF
                     ESRRPHSMILWGPPGVGKTTLARLMARGFDAEFVALSAVFSGVKEIREAIQQAQAAKA
                     RGRHTILFVDEVHRFNKAQQDAFLPYVEQGLVTFIGATTENPSFEVNSALLSRAAVYV
                     LESLDHDAMLRLFERAHAIACPTLGFDEDARERLIGFADGDARRLMNLIEQVQVAAET
                     AKVDPVSATFVDQALSRNLRRFDKGGEAFYDQISALHKSVRGSDPDAALYWLCRMLDG
                     GADPLYLGRRLIRMAVEDIGLADPRALEIALNACHTYERLGSPEGELALAQATIFMAC
                     AAKSNAAYKAYKAARAFVAHDGSRPVPLHLRNAPTKLMKALDYGKAYRYAHDEPEAYA
                     AGENYFPEGMQSPGWYQPTPRGLEGKIGQKLAWLRELDRKAAEGGKQYARPSPSAGGA
                     SREVPASKGTPGEERISGGGDDPS"
     gene            complement(2144..2407)
                     /locus_tag="PKV97_08850"
     CDS             complement(2144..2407)
                     /locus_tag="PKV97_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06023.1"
                     /translation="MLSIRDCLDYCDLTDEEVLLIAEHEDIPDAAAAQLCCGLVQTPE
                     GVLALTHYMRELIERANACGDVRKAKLAASVCERFMKDHPLPH"
     gene            2649..3941
                     /gene="serS"
                     /locus_tag="PKV97_08855"
     CDS             2649..3941
                     /gene="serS"
                     /locus_tag="PKV97_08855"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNB06024.1"
                     /translation="MLDIQLLRTQLDHVAARLATRGLQLDTAAFQALEDERKQLQTRT
                     QELQARRNALSKQIGMLKGKGEDASAVMAEVAQLGDGLKACEQALPMLLERINAFLAT
                     LPNLPQDDVPAGADETGNVEVRRWGTPRAYDFEVRDHVDLGAPLGLDFETGAKLSGSR
                     FAFLRGPVARLHRALAQFMLDTHTREHGYTECYTPYIVNGSALYGTGQLPKFKEDLFW
                     VLRGGDEEGLEQYLIPTAEITLTNSVREEVLALESLPIRLTAHSPCFRSEAGSGGRDV
                     RGMIRQHQFDKVEMVQIVEPAKSKEALEQMVGHAEAILQKLELPYRVITLCTGDMGFS
                     AAKTYDLEVWLPAQNTYREISSCSNCESFQARRMQARFKNAQGKNELVHTLNGSGLAV
                     GRTLVAVLENHQQADGSIVIPRALVPYMGGIERIEAPL"
     gene            complement(4051..5049)
                     /locus_tag="PKV97_08860"
     CDS             complement(4051..5049)
                     /locus_tag="PKV97_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNB06025.1"
                     /translation="MSDTHALLGLPAGASAQQIKRAFRKLAMRWHPDRNPDPAAADQF
                     RRLRDAHDRLLAALLEGKVKAHEDAAATQPPPQRGADRWQTLEISIEESFTGARRTVE
                     VERRSPCVLCGGSGIEKLSVSRLCTPCRGSGRLHGPAGLERCPDCEGRGYRNTGPCGG
                     CGGSGEQMQTRHLEVLIPPGVVDDDELRLAGEGEAHPEPRHRAGDLHLQIRLASHPLF
                     RREGRDLVLRRPVSALRLLIGGTLRVPHPTGVRSIELEPGLAHPRSLRVPGAGFPAHG
                     RRARGDLVVEFEPELPHAPERSLHGLIDELDRALAARAEHHFPELARWESVWLDEH"
     gene            5246..6085
                     /locus_tag="PKV97_08865"
     CDS             5246..6085
                     /locus_tag="PKV97_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB06026.1"
                     /translation="MSPSRASETQPRDSWVHHPQGRIFAREWTPSGAAGERPASPIVL
                     LHDSLGCVELWRDFPAELSAASGRRVIAYDRLGFGRSDAREALPALDFIAEEAQAYFP
                     AVREQLGLERFVVFGHSVGGGMAVNCAAAFAGACDALITESAQVFPEDLTLSSIAEAK
                     EQFVDESQLQRLARYHGDKARWVLDAWTESWLHPGFAAWSLRSVLPQVRCPVLAIHGM
                     HDEYGTSVHPETIGELSAGPAQVAILPDTFHVPHRERPAAVIELVCAFLEPVRQGGAV
                     CVS"
     gene            complement(6225..6301)
                     /locus_tag="PKV97_08870"
     tRNA            complement(6225..6301)
                     /locus_tag="PKV97_08870"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6265..6267),aa:Arg,seq:acg)
     gene            complement(6344..6420)
                     /locus_tag="PKV97_08875"
     tRNA            complement(6344..6420)
                     /locus_tag="PKV97_08875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6384..6386),aa:Arg,seq:acg)
     gene            complement(6483..6572)
                     /locus_tag="PKV97_08880"
     tRNA            complement(6483..6572)
                     /locus_tag="PKV97_08880"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6536..6538),aa:Ser,seq:cga)
     gene            complement(6713..7063)
                     /locus_tag="PKV97_08885"
     CDS             complement(6713..7063)
                     /locus_tag="PKV97_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNB06027.1"
                     /translation="MSKSHVQIVSDVSKALATLRKEIPETMQGFGAMAKSSLQDGVLS
                     ELQKELIALAIAVTQRCDACIGFHIKALIRLGATREQIMETLGVCTYMGGGPALMYAA
                     EAVRAYEEMLPQAR"
     gene            complement(7190..>7368)
                     /locus_tag="PKV97_08890"
     CDS             complement(7190..>7368)
                     /locus_tag="PKV97_08890"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HNB06028.1"
                     /translation="TERAYDNPKFVEDMVRDVAGQLNREPRIDAYVVESENFESIHNH
                     SAYALIERDKRGGA"
BASE COUNT         1155 a         2499 c         2535 g         1179 t
ORIGIN      
        1 ccggccaagg agagcgaatg atccagttca acacacgacc ggccgcgctg ctgcgcggcg
       61 tggcgggcgt cgtgctcgcg ctggcggccc tgggcgccac ggcggcgggt ggcgtgcagc
      121 agctgcgcga cttcgtcgcc ggcgcgcgca gcgcggaggg cgagttcgag caggtcgtca
      181 ccgcgcagtc cgggcgccga cctcaggagg cctcgggcag cttcgcctac gcccgcccgg
      241 ggcgcttcca ttgggagtac gaccggcctt atcgccaggt gctggtgggc gacggcaaga
      301 ccctgtggtc ctgggatccc gacctcaacc aggtgaccgt acgcgcgatc ggcgaggcgc
      361 tcggcgccac cccggcggcg atcctgttcg gcagcggcgc gctcgacgac aatttcgagc
      421 tcgccgacgc cggcgagcgg gacggtctgg cctgggccga ggcgcgtccg aagcgcaccg
      481 agagcggctt cgagtcgctg cgcatcgggc tggctgaagg ccagttgcgg cgtatggaga
      541 tgcgcgacca cttcggccag accacggtga tccgcttcac ccgcctgcgc gccaacccgg
      601 tgctcgccga ggaccgcttc cgcttcgagc cgcccgcagg cgccgacgtg atcggcgaac
      661 tgcccggcgc gcgctgactc cccacgcacg cgtgcgcacg ccgcaatcga tccccgagtc
      721 catgggccgt ctgcaagggg gctccgcatg accgacctgt tcgacgccct cgagccgccg
      781 caggtgccgc tcgctgagcg catgcggccg cacaccctcg acgaggtcgc cggccagtca
      841 cacctgttgg gaccgggcaa gccgctgcga ctggccttcg agtcgcgccg gccgcactcg
      901 atgatcctgt ggggcccgcc cggcgtcggc aagaccacgc tcgcacggct gatggcgcgc
      961 ggcttcgacg ccgagttcgt cgccctgtcc gcggtgttct ccggggtcaa ggagatccgc
     1021 gaggcgatcc agcaggccca ggccgccaag gcgcgcggtc gccacaccat cctcttcgtc
     1081 gacgaggtgc accgcttcaa caaggcgcag caggacgctt tcctgcccta cgtcgagcag
     1141 ggcctggtga ccttcatcgg cgccaccacc gagaaccctt cgttcgaggt caactccgcg
     1201 ctgctgtcgc gcgccgcggt gtatgtgctg gagtcgctcg accacgatgc catgctgcgc
     1261 ctgttcgagc gcgcgcacgc gatcgcctgc ccgacgctcg gcttcgacga ggatgcgcgc
     1321 gagcgcctga tcggctttgc cgacggcgat gcgcgcaggc tgatgaacct gatcgaacag
     1381 gttcaggttg ccgccgagac cgcgaaggtc gatccggtga gcgccacctt cgtcgaccag
     1441 gccctgtcgc gcaacctgcg ccgcttcgac aagggcggag aggccttcta cgaccagatt
     1501 tccgcgctgc acaagtcggt gcgcggctcg gatccggatg ccgcgctgta ctggctgtgc
     1561 cgcatgctcg acggcggcgc cgatccgctc tacctcggcc ggcggctgat ccgcatggcg
     1621 gtggaggaca tcggcctggc cgatccgcgt gcgctcgaga tcgcgctcaa tgcctgccac
     1681 acctacgagc gcctcggttc tcccgagggc gaactcgcgc tcgcccaggc gacaatcttc
     1741 atggcctgcg cggccaagtc caacgccgcc tacaaggcct acaaggccgc gcgcgccttt
     1801 gtcgcccacg acggctcgcg tccggtgccg ctgcacctgc gcaacgcgcc gaccaagctg
     1861 atgaaggcgc tcgactacgg caaggcctac cgctacgcgc acgacgagcc cgaggcctat
     1921 gccgcgggcg agaattattt ccccgaggga atgcagtcgc ccgggtggta ccaacccact
     1981 ccgcgcggcc tcgaaggcaa gatcggccag aagctcgctt ggctgcgcga actcgaccgc
     2041 aaggcggcgg agggcggaaa acaatacgcg aggccgtcgc catctgcggg cggcgcctcg
     2101 cgcgaagtcc cggcgagcaa aggtacgccg ggcgaggaga gaatcagtgg gggaggggat
     2161 gatccttcat gaaacgctcg cagacggaag ccgccagttt ggccttgcgc acgtccccgc
     2221 atgcattggc acgctcgatg agttcgcgca tgtagtgcgt gagtgccagc acgccctccg
     2281 gcgtctgcac gagcccgcag caaagctgcg cggcggcggc atccgggatg tcctcatgct
     2341 cggcaatcaa caacacctcc tcgtcggtga gatcgcagta gtccaggcag tcgcgaatgg
     2401 atagcatgcc gtttcctcca ggtccgggcc gtctgatgcg gctttgtcgt caactcaagg
     2461 atagtggacc gatccgcttt tacaagttcg atagaaattc agaaccagaa ccaagtcttt
     2521 gattaagaaa gccgattttg cgaagcgcga ggtcttgtcg cgcaaccccg ccatgtaatc
     2581 agtcgcctgc gacggttttc cgagaatccg catcaacttt tgccgatcgc ttttcaggaa
     2641 cgtttccgat gctcgatatc cagcttctcc gcacccagct cgaccacgtc gccgcacgcc
     2701 tcgccacgcg cgggctgcag ctcgacaccg ccgccttcca ggccctcgag gacgagcgca
     2761 agcagttgca gacccgcacc caggaactgc aggcccgccg caacgcgctg tccaagcaga
     2821 tcggcatgct caagggcaag ggcgaggacg cctccgcggt gatggccgag gtcgcccagc
     2881 tcggcgacgg gctcaaggcc tgcgagcagg ccctgccgat gctgctcgag cgcatcaacg
     2941 ccttcctggc caccctgccg aacctgccgc aggatgatgt tccggcgggc gcagacgaga
     3001 ccggcaacgt cgaggtgcgg cgctggggta cgccgcgcgc ctacgacttc gaggtgcgcg
     3061 accacgtgga cctcggcgct ccgctcggcc tcgacttcga gaccggcgcc aagctgtcgg
     3121 gctcgcgctt cgccttcctg cgcggtccgg tggcgcgcct gcatcgcgcg ctcgcgcagt
     3181 tcatgctcga cacccacacc cgcgagcacg gctacaccga gtgctacacg ccctacatcg
     3241 tcaatggctc ggcgctctac ggcaccggtc agctgcccaa gttcaaggag gatctgttct
     3301 gggtgctgcg cggcggcgac gaggagggcc tcgagcagta cctgatcccg accgcagaga
     3361 tcacgctcac caacagcgtg cgcgaggagg tgctcgcgct cgagagcctg ccgatccgtc
     3421 ttaccgcgca tagcccctgc ttccgctccg aggcgggcag cggcgggcgc gacgtgcgcg
     3481 gcatgatccg ccagcaccag ttcgacaagg tcgagatggt gcagatcgtc gagccggcga
     3541 agagcaagga ggcgctcgaa cagatggtcg gccacgccga ggccatcctg cagaagctcg
     3601 agctgcccta ccgggtgatc acgctgtgca ccggcgacat gggcttctcg gctgcgaaga
     3661 cctacgatct cgaggtgtgg ctgccggcgc agaacacata ccgcgagatc tccagctgct
     3721 cgaactgcga gtccttccag gcgcgccgca tgcaggcccg cttcaagaac gcgcagggca
     3781 agaacgagct ggtgcacacg ctcaacgggt ccggtctggc ggtggggcgc accctggttg
     3841 cggtgctgga gaaccaccag caggccgacg gttcgatcgt gatcccgcgg gcgctggtgc
     3901 cctacatggg tggcatcgag cgcatcgaag cgccgctctg agcggcgatc ggcggttggt
     3961 gcgggtccga ccttgcggcc cgcgccagcc gcgtcctggc ggggccgtgc cgtgtgcgat
     4021 gccgtcccgc tccgccgctt gcgcccgcgc tcagtgctcg tccagccaca ccgactccca
     4081 gcgcgcgagc tcggggaagt ggtgctcggc gcgcgcggcg agcgcgcggt cgagttcgtc
     4141 gatcaggccg tgcagcgagc gttcgggcgc gtgggggagt tcgggctcga actccacgac
     4201 gaggtcgcca cgtgcgcgcc ggccgtgcgc cggaaagccg gcgccgggta cgcgcaggct
     4261 gcgcggatgg gcgaggccgg gctcgagttc gatgctgcgc acgcccgtcg ggtggggcac
     4321 gcgcagggtc ccgccgatca gcaggcgcag cgcgctgacc gggcggcgca gcaccaggtc
     4381 gcggccctcg cggcgaaaca gcggatgact ggcgaggcgg atctgcaggt gcaggtcgcc
     4441 agcgcgatgg cgcggctcgg ggtgcgcctc gccctcgccg gcgagacgca gttcgtcgtc
     4501 gtccaccacc ccgggtggaa tgagcacctc gagatgacga gtctgcattt gttccccgct
     4561 gccgccacag ccgccacacg gtcccgtgtt gcgatagccc cgcccctcgc agtccgggca
     4621 gcgctccagc cccgcaggtc cgtgcaagcg gcccgagcct cggcagggtg tgcataggcg
     4681 gctcacgctg agcttctcga tgccgctgcc gccgcacagc acgcaggggc tgcggcgctc
     4741 gacctcgacc gtccgccgcg ccccggtgaa gctctcctcg atgctgatct ccagtgtctg
     4801 ccagcggtcc gcgccgcgct gcggcggagg ctgggtggca gccgcgtcct cgtgcgcctt
     4861 caccttgcct tcgagcaggg cggcgagcag gcggtcgtgc gcatcgcgca gccgcctgaa
     4921 ctgatcggcg gcggcggggt cgggattgcg atcgggatgc catcgcatgg cgagcttgcg
     4981 gaaggcgcgc ttgatctgtt gcgcgctcgc gccggctggc aggccgagca gtgcgtgggt
     5041 gtcggacatg gacgaatggg tgaggaccgc gagggcatca gcttaaccga gcttgcccgc
     5101 ggatgctgcg cccgcacttg acggatctcc caaggatgaa agaatgtggc gccgtgttca
     5161 atacggaatc gtatggcctg gtgctccggc agaactcgcc ggagcgcgaa cacgcggaaa
     5221 atgaatacat ggagacgacg aaacgatgag cccaagccga gcttccgaaa cccagccccg
     5281 cgactcgtgg gtgcatcatc cgcaggggcg catcttcgcg cgggaatgga ccccgtccgg
     5341 ggcggccggc gagcggccgg cgagcccgat cgtgctgctc cacgactcgc tcggctgtgt
     5401 cgagctgtgg cgcgacttcc cggcggagct gagcgccgcc agcgggcgcc gggtgatcgc
     5461 ctacgaccgc ctcgggttcg gccgctcgga tgcgcgcgag gcgctgccgg cgctggactt
     5521 catcgccgag gaggcgcagg cgtattttcc ggcggtgcgc gagcagctcg gcctggagcg
     5581 cttcgtggtg ttcggccaca gcgtcggcgg cggcatggcg gtgaattgcg cggctgcatt
     5641 tgccggggcc tgcgacgccc tgatcaccga gtcggcccag gtcttccccg aggacctcac
     5701 cctgagcagc atcgccgagg ccaaggagca gttcgtcgat gaatcgcagc tgcagcgcct
     5761 ggcgcgctat cacggcgaca aggcgcgctg ggtgctggat gcgtggaccg agagctggtt
     5821 gcatcccggc ttcgcggcct ggtcgctgag gtcggtgctg ccgcaggtgc gttgccctgt
     5881 gctggccatc catggcatgc atgacgagta cggcacgagc gtgcaccccg agacgatcgg
     5941 cgagctctcc gcgggcccgg cgcaggtggc gatcctgccg gacaccttcc acgtgccgca
     6001 tcgcgagcgt ccggccgcgg tcatcgaact cgtctgcgcc ttcctcgagc cggtgcggca
     6061 gggcggggcg gtgtgcgtgt cctgagcggt cggcgcggtg catccgggcg ggtcccggat
     6121 gggccgctcg ggtatcgggc gggggcgcgc cgatgcgacg aggagggacg gaaaaggagg
     6181 acgaaaaaac gccgacccgg tgaagagtcg gcgtttttcg aaactggcgc gcccggaagg
     6241 attcgaacct cctaccccct ggttcgtagc caggtactct atccagatga gctacgggcg
     6301 cgttgccgaa atcgtttaga gcccgaaatc ggaaggcttt ttttggcgcg cccggaagga
     6361 ttcgaacctc ctaccccctg gttcgtagcc aggtactcta tccagatgag ctacgggcgc
     6421 ttcttgctgc actgcgaaga accgggatta taacgactgt tcccgcaact tcaagtttaa
     6481 attggcggag agggtgggat tcgaacccac ggtaggcttg cacctacgcc tgatttcgag
     6541 tcaggtacat tcgaccactc tgccacctct ccgctcgaag gtcggcatta tagtgttttt
     6601 ttccgggaag tgaacagttt tttgcggggg gtaaagtgaa gagaccccgt cgccggcccc
     6661 gacccggcgc atcgggacgg gcaaggcggc cgcggtcggg cccgggctgc gctcacctgg
     6721 cctgcggcag catctcctca taggcgcgca ccgcctccgc agcgtacatc agggccgggc
     6781 cgccgcccat ataggtgcac acgcccaggg tctccatgat ctgctcgcgg gtcgcgccga
     6841 ggcggatcag cgccttgatg tggaagccga tgcaggcgtc gcagcgctgg gtgacggcga
     6901 tcgcgagtgc gatgagttcc ttctgcagct cgctcagcac gccgtcctgc agcgagctct
     6961 tggccatcgc gccgaagccc tgcatggtct cggggatctc cttgcgcagc gtggcgagcg
     7021 ccttggagac atcgctcacg atctggacgt gggacttgga catcatgcgt tctcctattt
     7081 cagaaaacac ttatcttact ccgccgacgc gcttgcgacc atcgcgtcgc ggcgccgggc
     7141 gtgcgtgccc gagcgcaccc gcgacacggc cccgctaaag gggcgggggt caggcgccgc
     7201 cgcgcttgtc gcgctcgatc agcgcgtaag cggagtggtt gtggatcgac tcgaagttct
     7261 cggactcgac cacgtaggcg tcgatgcgcg gctcgcggtt cagctggccg gcgacgtcgc
     7321 gcaccatgtc ctcgacgaac ttggggttgt cgtaggcgcg ctcggtga
//
LOCUS       DAOQNN010000139         7346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5320_length_7346_cov_10.764230, whole genome shotgun sequence.
ACCESSION   DAOQNN010000139 DAOQNN010000000
VERSION     DAOQNN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5320_length_7346_cov_10.764230"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..287)
                     /locus_tag="PKV97_08895"
     CDS             complement(96..287)
                     /locus_tag="PKV97_08895"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HNB06029.1"
                     /translation="MPLAEITMMEGRTEDQKRAVIQKVTQAIVEAVGAPIESVRVVIR
                     EVPKTNWGIGGVSAKDLGR"
     gene            complement(386..1417)
                     /gene="dmpG"
                     /locus_tag="PKV97_08900"
     CDS             complement(386..1417)
                     /gene="dmpG"
                     /locus_tag="PKV97_08900"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HNB06030.1"
                     /translation="MELRGKKITVHDMTLRDGMHPKRHLMTLEQMKSIATGLDAAGVP
                     LIEVTHGDGLGGSSVNYGFPAHSDEEYLGTVIPLMKQAKVSALLLPGIGTVDHLKMAH
                     ELGVSTIRVATHCTEADVSEQHIGMARKLGMDTVGFLMMAHMNSPEGLVTQARLMESY
                     GANCIYVTDSAGHLLPDTVKARLSAVRDALKPETELGFHGHHNLAMGVANSLAALEVG
                     ATRIDAAAAGLGAGAGNTPLEVFIAVCDLMGIETGVDVFKIQDVAEDLVVPIMDFPIR
                     IDRDALTLGYAGVYGSFLLFAKRAEKKYGVPAREILVEMGKRGMVGGQEDMIEDTALN
                     LAKARGLAV"
     gene            complement(1442..2365)
                     /locus_tag="PKV97_08905"
     CDS             complement(1442..2365)
                     /locus_tag="PKV97_08905"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HNB06031.1"
                     /translation="MNKIKCALIGPGNIGTDLLYKLKRSPVLEPVWMVGIDATSEGLA
                     RARELGLKTTADGVDGLLPHVKADGVQIAFDATSAYVHAENSRKLNELGVLMIDLTPA
                     AIGPFCVPPVNLVEHVGKGEMNVNMVTCGGQATIPMVAAVSRVQPVAYGEIVATVSSK
                     SAGPGTRKNIDEFTRTTAGAVEKVGGARKGKAIIILNPAEPPLIMRDTVHCLTETEPD
                     REKITASIHAMIQEVQKYVPGYRLVNGPVFDGNRVSVFLEVEGLGDYLPKYAGNLDIM
                     TAAGARTAEMFAEEILAGRLQLESNRATMVA"
     gene            complement(2506..2943)
                     /locus_tag="PKV97_08910"
     CDS             complement(2506..2943)
                     /locus_tag="PKV97_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNB06032.1"
                     /translation="MKVAVPQATISWEAAHAAVAAAVEKAEALGVRINVGVADASGNL
                     VAFLRMPGAFPQSIAIAIDKAYTAGGFGFPTGKWMDVCAGNEGMKLGFSAQPRLVVFG
                     GGLPIRVGGELVGGIGVSGASEDQDGICARAGLLAIGADDVEP"
     gene            3104..3823
                     /locus_tag="PKV97_08915"
     CDS             3104..3823
                     /locus_tag="PKV97_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNB06033.1"
                     /translation="MNEAVPQRTPEAVEPKTLVEGAYQRLRRDIIEGVHPPGEKLRVE
                     HLKDRYEVGAGTLREALLLLVTDALVVAQGQRGFRVSPISIEDFEDITRTRLLLETEA
                     LRQSLQLGGEDWEASVVAAFHRLSRAEQKLGDHDSLAAEEWERRNRAFHEALIAASPS
                     RWIRHFQHILYQQSERYRRLSLFSHPIPRDIHAEHQALFNASLARDTTRATSILTEHV
                     LRTLDAVKLLPAEFFAGKHKS"
     gene            complement(3911..4840)
                     /locus_tag="PKV97_08920"
     CDS             complement(3911..4840)
                     /locus_tag="PKV97_08920"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HNB06034.1"
                     /translation="MAMTGVLRPGHISLRVLDLEEGINFYKNTLGLVETGRDGQGRVY
                     FKAWDEREHNSVLIREAESAGVDFFAFKVADKATLDKLDADLQAFGLKTERIPAGEML
                     ETGERVRFEVPSGHLIELYAEKTDIGNGQSYVNPDPWIPDAERGIAPSRMDHCLLYGP
                     DIEKVQEIFEKVLGFYLVEHVVMEDGKTDLAIWLSCSTKAHDIAFVRHPEPGKLHHIS
                     FKLDSWEKVLRAADIMSMNRISIDIGPTRHGITRGTTIYAFDPSGNRFETFCGGYDTY
                     PDYKTITWTWDEVGAGIFYHDRKLNERFLSVVS"
     gene            complement(4970..5884)
                     /locus_tag="PKV97_08925"
     CDS             complement(4970..5884)
                     /locus_tag="PKV97_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB06035.1"
                     /translation="MSIMSSAQPAAPATAADDPEIGRSIRAAGILTNYHDLGEGFPAL
                     FIHGSGPGVSAWANWRLVLAPLAQRMRVIAPDMAGFGFTERRADLRYDIDTWVAQALG
                     LMDALGLERADVVGNSFGGALALALAIRHPQRVRRLVLMGSVGVHFPITPGLDAVWGY
                     TPSFENMRRIMDCFAWDRSLVNDELAELRYRASVRPGFQESFASMFPAPRQRWVDALA
                     SREEDIRALPHETLIIHGRDDQVIPLQTSETLLRWIERAQLHVFGRCGHWTQIEHSER
                     FVRLVGDFLCEEGERRAWPAAGQAEGGT"
     gene            complement(5904..6959)
                     /locus_tag="PKV97_08930"
     CDS             complement(5904..6959)
                     /locus_tag="PKV97_08930"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenol 2-monooxygenase domain-containing
                     protein"
                     /protein_id="HNB06036.1"
                     /translation="MSYELTIEPIGQTIEVEEGQTILDAALRAGIWLPHACCHGLCAT
                     CKVQVLDGEIDHGEASSFALMDFERDEGKTLACCARLESDVTIEAEIEEDPDAESIPV
                     RDFPGTVTRIEALTPTIKAVFIETDEPMHFQAGQYINFFLEGGEYSRAFSLANPPSTG
                     REVELNIRIVPGGRGTTWVHEQLQVGERVRLSGPYGRFFVRKSAPEALLFMAGGSGLS
                     SPRSMILDLLEAGDTRQMTLVYGQRNRAELYYHDEFLALAAKHSNFTYVPALSDEPAD
                     SGWEGFRGYVHEAATAHFDNDFRGHKAYLCGPPLMIDACITALMQGRLFERDIYMEKF
                     FSAADVQQVRSPLFKKI"
     gene            complement(7035..>7346)
                     /locus_tag="PKV97_08935"
     CDS             complement(7035..>7346)
                     /locus_tag="PKV97_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase subunit P4"
                     /protein_id="HNB06037.1"
                     /translation="QDKFHGAQLLYIGWEDHLLFCAPFAFPFPPTMRFGDVVAGPMQA
                     AFGYHPDYAQIDWAKAEWFKSGKPFAPDFDKSLAENGLRHKDVIRFRTPGLTGIKGSC
                     S"
BASE COUNT         1117 a         2457 c         2494 g         1278 t
ORIGIN      
        1 tcctccttga ttggtcttgc gacgagtgtg ctgctcctgg accggcgccc tctgcggggc
       61 gctcggtgaa tctcattctc gcgggtgcac gaccgtcagc gcccgaggtc cttcgccgac
      121 accccgccga tgccccagtt ggtcttgggc acctcgcgga tgacgacgcg cacgctctcg
      181 atcggagcgc cgacggcctc gacgatcgcc tgggtcacct tctggatcac tgcgcgcttc
      241 tggtcttcgg tacggccttc catcatggtg atttctgcca gcggcatggt ctcttcctcc
      301 ggttgtcgaa ctattgcgag ccggcgcccc cggcgtccgg cccgcggaaa acgaccggtg
      361 cggcccgcgc cgcaccggcg gcgactcaca ccgccaggcc gcgcgccttg gcgaggttga
      421 gcgcggtgtc ctcgatcatg tcttcctggc cgccgaccat gccgcgcttg cccatctcga
      481 ccaggatctc gcgcgccggc acgccgtact tcttctcggc gcgcttggcg aacagcagga
      541 aggagccgta caccccggcg tagccgagcg tgagcgcatc gcggtcgatg cggatcggga
      601 agtccatgat cggcaccacc aggtcttcgg ccacgtcctg gatcttgaac acatccacgc
      661 cggtctcgat gcccatcagg tcgcacaccg cgatgaagac ctccagcggg gtgttgcccg
      721 cgccggcacc caggcccgcg gcggccgcgt cgatgcgggt ggcacccact tccagcgcgg
      781 ccaggctgtt ggccacgccc atggcgaggt tgtggtggcc gtggaagccg agttcggtct
      841 cgggcttgag cgcgtcacgc accgcggaca ggcgtgcctt caccgtgtcg ggcagcaggt
      901 ggccggccga gtcggtgacg tagatgcagt tggcgccgta gctctccatc agcctggcct
      961 gcgtgaccag gccttcgggg ctgttcatgt gcgccatcat caggaagccg acggtgtcca
     1021 tgcccagctt gcgcgccatg ccgatgtgct gctcggagac gtcggcctcg gtgcagtggg
     1081 tggcgacgcg gatggtggac acgccgagct cgtgcgccat cttgaggtga tcgacggtgc
     1141 cgatgcccgg cagcagcagc gccgagacct tggcctgctt catcagcggg atcacggtgc
     1201 cgaggtattc ctcgtcgctg tgcgccggga agccgtaatt gaccgagctg ccaccgaggc
     1261 cgtcgccgtg ggtgacctcg atcagcggta cgcccgccgc atcgagcccg gtggcgatcg
     1321 atttcatctg ctcgagcgtc atcaggtggc gcttggggtg catgccgtcc cgcagggtca
     1381 tgtcgtggac ggtgatcttc ttgccgcgaa gttccatgtt catctccttg tgccggggcg
     1441 ctcaggccac catcgtggcg cggttggatt cgagctgcag acgcccggcg aggatctcct
     1501 cggcgaacat ctcggcggtg cgcgcgccgg ccgcagtcat gatgtcgagg ttgccggcgt
     1561 acttgggcag gtagtcgccc aggccttcga cttccaggaa gacggagacg cggttgccgt
     1621 cgaacaccgg gccattgacc aggcggtagc cgggcacgta cttctgcact tcctggatca
     1681 tggcgtggat cgaggcggtg atcttctcgc ggtcgggttc ggtttcggtg aggcaatgca
     1741 ccgtgtcgcg catgatcagc ggcggttcgg ccggattcag gatgatgatg gccttgccct
     1801 tcctggcgcc gcccaccttc tcgaccgcgc cggcagtggt gcgggtgaat tcgtcgatgt
     1861 tcttgcgcgt gccgggcccg gcactcttgg aggacacggt ggcgacgatc tcgccgtagg
     1921 ccaccggctg cacgcgcgac accgccgcga ccatcgggat cgtggcctgg ccgccgcagg
     1981 tgaccatgtt cacgttcatc tcacccttgc cgacgtgctc gacgaggttc accggcggca
     2041 cgcagaaggg gccgatcgcg gccggggtga ggtcgatcat cagcacgccg agctcgttga
     2101 gcttgcggct gttctcggca tgcacatagg ccgaggtggc gtcgaaggcg atctgcacgc
     2161 cgtcggcctt cacatgcggc agcagcccgt cgacgccgtc ggcggtggtc ttgaggccga
     2221 gctcgcgcgc gcgcgcgagg ccttcggagg tggcgtcgat gcccaccatc cacaccggct
     2281 cgagcaccgg gctacgcttc aacttgtaga gcaggtcggt gccgatgttg cccggtccga
     2341 tcagcgcaca cttgatcttg ttcatgcctg tcttcctttg gggttgagtc gtttccagga
     2401 atgcaggccg cgcgacggtc cggggagagg ggagagagag acggaccgcc gcacgactgc
     2461 aaccgggaga tgcccgcagt gcggacgaaa aaaagaactc gccgctcagg gctcgacgtc
     2521 gtcggcgccg atcgcgagca gccctgcgcg tgcgcagatg ccatcctggt cctcggaggc
     2581 acccgaaacg ccgatgccgc cgaccagctc gccgccgacg cggatcggca ggccgccgcc
     2641 gaacacgacc agccgcggct gtgccgagaa gcccagcttc atgccctcgt tgccggcgca
     2701 cacgtccatc cacttcccgg tcgggaagcc gaagccgccg gcggtgtagg ccttgtcgat
     2761 ggcgatcgcg atcgactgcg ggaaggcgcc gggcatgcgc aggaaagcga cgaggttgcc
     2821 ggaggcgtcg gcaacaccga cgttgatccg caccccgagc gcctcggcct tctcgaccgc
     2881 ggcggcgacg gcggcatgtg ccgcctccca gctgatggtg gcctgaggaa cagcgacttt
     2941 catcgggtga gctcctgtgg ttcaggtttg atttgtaatg tttttgaaga taggccaggg
     3001 caatgtcgac gtcaaggatt tttctcgaga tttatagaag aagctaattc catcccgctc
     3061 ggatagaatc gggaaccggg gtgtttccaa cattttcgca atcatgaacg aagccgttcc
     3121 gcaaagaaca ccggaggccg tcgagcccaa gaccctcgtc gagggggcct accagcgcct
     3181 gcgccgggac atcatcgagg gcgttcaccc gcccggcgag aaacttcgcg tcgaacatct
     3241 caaggaccgc tacgaggtcg gcgccggcac cctgcgcgag gccctcctcc tgctcgtcac
     3301 cgacgccctg gtggtcgccc agggacaacg cggcttccgc gtctcgccga tttccatcga
     3361 ggacttcgag gacatcaccc gcacccgcct gctgctcgag accgaagccc tgcgccagtc
     3421 gctgcagctc ggcggcgagg actgggaggc ctccgtggtc gcggctttcc accgcctctc
     3481 tcgcgccgag caaaagctcg gcgaccacga ctcgctggcc gccgaggagt gggagcggcg
     3541 caaccgcgcc ttccacgagg cactgatcgc cgcgtcgccc tcgcgctgga tccgtcattt
     3601 ccagcacatc ctctaccagc agtccgagcg ctaccggcgc ctgagcctgt tcagccaccc
     3661 catcccgcgc gacatccacg ccgagcacca ggccttgttc aacgccagcc tggcacgcga
     3721 caccacgcgc gcgacctcca tcctcaccga gcacgtgctg cgcacgctcg acgcggtgaa
     3781 gctgcttccg gcggagttct tcgccggcaa gcacaagtcc tgagttcgcc ggacgcacgc
     3841 cccgttcaac ctgcctgcgc gcccaccggc gcccctgccc gatcggcagg gagcgccggc
     3901 gcggccgcgg tcaggacacc acgctcagga agcgctcgtt gagcttgcgg tcgtgataga
     3961 agatcccggc gcccacctcg tcccaggtcc acgtgatggt cttgtagtcg gggtaggtgt
     4021 cgtagccgcc gcagaaggtc tcgaagcggt tgcccgaggg gtcgaaggca tagatcgtgg
     4081 tgccgcgggt gatgccgtgg cgggtcgggc cgatgtcgat cgagatgcgg ttcatcgaca
     4141 tgatgtcggc cgcgcgcagc accttctccc agctgtcgag cttgaacgag atgtggtgca
     4201 gcttgcccgg ctcggggtgg cgcacgaagg cgatgtcgtg ggccttggtc gagcacgaca
     4261 gccagatcgc caggtcggtc ttgccgtctt ccatcaccac gtgctcgacg aggtagaagc
     4321 cgagcacctt ctcgaagatc tcctgcacct tctcgatatc cggcccgtac agcaggcagt
     4381 ggtccatgcg cgagggcgcg atgccacgct cggcatccgg gatccacggg tccgggttca
     4441 cataggactg gccgttgccg atgtcggtct tctcggcata gagctcgatc aggtggcccg
     4501 acggcacctc gaagcgcacg cgctcgccgg tctcgagcat ctcgccggcg gggatgcgct
     4561 cggtcttcag gccgaaggcc tgcaggtcgg cgtcgagctt gtcgagcgtc gccttgtcgg
     4621 cgaccttgaa ggcgaagaag tccacgccgg cgctctcggc ctcgcggatc agtacgctgt
     4681 tgtgctcgcg ctcgtcccag gccttgaagt agacgcggcc ctggccgtcg cggccggttt
     4741 cgaccaggcc gagggtgttc ttgtagaaat tgatgccttc ctcgaggtcg aggacacgca
     4801 gggagatgtg tcccggacga agtacgcctg tcattgccat gttgtctgct cctctcttga
     4861 tatgcccggg acggcacgcc cgcccgggtc ggtttgctcg atcttcatcc gtcgttcgac
     4921 ggatcctgcc gtggcccgtg ccgccctgcg ggcgcgcgcc acgaagcctt caagtaccgc
     4981 cttcagcctg ccctgccgcc ggccaggctc ggcgctcgcc ctcctcgcag aggaagtcgc
     5041 cgaccaggcg cacgaagcgc tcggagtgct cgatctgcgt ccagtgcccg cagcggccga
     5101 acacgtgaag ttgtgcgcgc tcgatccagc gtagcagggt ctccgaggtc tgcagcggaa
     5161 tcacctggtc gtcgcggcca tggatgatca gggtctcgtg cggcagcgcg cggatgtcct
     5221 cctcgcgact ggccagcgca tcgacccagc gctggcgcgg cgcggggaac atgctcgcga
     5281 aggactcctg gaagcccgga cggacgctgg cgcggtagcg cagttcggca agctcgtcgt
     5341 tcaccaggct gcggtcccag gcgaagcagt ccatgatccg gcgcatgttc tcgaacgagg
     5401 gtgtgtagcc ccacaccgcg tccagccccg gcgtgatcgg aaagtgcacg cccacgctgc
     5461 ccatcagcac cagccggcgc acgcgctgcg ggtggcggat cgccagcgcc aacgcgagcg
     5521 cgccgccgaa ggagttgcct accacgtctg cccgctccag cccgagcgca tccatcagcc
     5581 cgagcgcctg cgcgacccag gtgtcgatgt cgtagcgcag gtcggcacgc cgctcggtga
     5641 agccgaagcc cgccatgtcg ggcgcgatca cccgcatgcg ctgcgcgagc ggcgccagca
     5701 ccaggcgcca gttggcccag gcggagacgc cgggacccga gccgtggatg aagagcgcgg
     5761 gaaagccctc gccgaggtcg tggtagttgg tcaggatgcc ggccgcgcgg atgctgcggc
     5821 cgatctccgg atcgtccgca gcggtcgctg gcgccgccgg ttgcgcgctg gacatgatgc
     5881 tcatccgtcc gtcctcgccc gtctcagatc ttcttgaaca gcgggctgcg tacctgctgc
     5941 acgtccgccg cggagaagaa cttctccatg tagatgtcgc gctcgaacag gcgtccctgc
     6001 atcagcgcgg tgatgcaggc gtcgatcatc agcggcgggc cgcacaggta ggccttgtgg
     6061 ccgcggaagt cgttgtcgaa gtgtgccgtc gccgcctcgt gcacatagcc gcggaagcct
     6121 tcccagcccg aatccgcggg ctcgtcggac agcgccggca cgtaggtgaa gttggagtgc
     6181 ttcgccgcga gggcgaggaa ttcgtcgtgg tagtagagct cggcgcggtt gcgctggccg
     6241 tacaccaggg tcatctgccg tgtgtcgccg gcctcgagca ggtcgaggat catcgagcgc
     6301 gggctcgaca gacccgagcc cccggccatg aacagcagcg cctcgggcgc cgacttgcgc
     6361 acgaagaagc gtccgtaggg accggacagg cgcacgcgct caccgacctg aagctgctcg
     6421 tggacccagg tcgtgccgcg gccgccgggc acgatgcgga tgttgagctc gacctcgcgg
     6481 ccggtggacg gcgggttggc gagcgagaag gcgcggctgt attccccgcc ctcgaggaag
     6541 aagttgatgt actggccggc ctggaaatgc atcggctcgt cggtctcgat gaagaccgcc
     6601 ttgatcgtcg gcgtcagcgc ctcgatccgc gtcacggtgc cggggaagtc gcgcaccggg
     6661 atcgactcgg cgtcgggatc ttcttcgatc tcggcctcga tggtcacgtc ggactcgagg
     6721 cgggcgcaac aggccagcgt cttgccctcg tcgcgctcga agtccatcag cgcgaagctg
     6781 gaggcctcgc cgtggtcgat ctcgccgtcg agcacctgca ccttgcaggt ggcgcacagg
     6841 ccgtggcagc aggcgtgggg cagccagatc cccgcgcgca gggcggcgtc gaggatggtc
     6901 tggccctcct ccacctcgat cgtctggccg atcggttcaa tcgtcagttc gtagctcatg
     6961 gtgcgcttcc gttcttgtgc gttctctggc ggggtgcgcg gggtgcggcg cgcgccgcac
     7021 cccgtggggg gcgatcagct gcaggagccc ttgatgccgg tgaggccggg cgtgcggaag
     7081 cggatcacgt ccttgtgcct gagcccgttc tcggccagcg acttgtcgaa gtcgggcgcg
     7141 aacggcttgc ccgacttgaa ccactcggcc ttcgcccagt cgatctgcgc gtagtcgggg
     7201 tggtagccga aggcggcctg catcggcccg gcgacgacgt cgccgaagcg catggtcggc
     7261 gggaagggga aggcgaaggg cgcgcagaac agcaggtggt cttcccagcc gatgtacagc
     7321 agctgggcgc cgtggaactt gtcctg
//
LOCUS       DAOQNN010000140         7297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5387_length_7297_cov_8.690831, whole genome shotgun sequence.
ACCESSION   DAOQNN010000140 DAOQNN010000000
VERSION     DAOQNN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5387_length_7297_cov_8.690831"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..549)
                     /locus_tag="PKV97_08940"
     CDS             complement(157..549)
                     /locus_tag="PKV97_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06038.1"
                     /translation="MDKANVSAGELAGQQAVELGELLARTVVSVAAAQEALDRHATAR
                     LDAIERGDEDAPRVPPLWYTFTEVTLEVALSASVERASGSVPPRLMSRPVDPRQVSLY
                     GYQAASSLMVRLRLAPREGMPDVSPPSA"
     gene            complement(602..1495)
                     /locus_tag="PKV97_08945"
     CDS             complement(602..1495)
                     /locus_tag="PKV97_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06039.1"
                     /translation="MPDLLPPLARALAEDANVSDELRGAGIAFGTLVERTGAAVAATQ
                     LQLDTSSAATASALAATLVNVIAVREQVFRDDGTLDHVTNHVRPLPLISFIDPVFYQW
                     TSVRLQGEFYAREFAASSETTSAYSGSNFGLFGSGVSMFFGPGMVTSGSGSGSSTTTT
                     DVSTDVSLGRIRASALLEPRDDVGIPKPNQVIRGPRLALIQGEIADVMDGGDLVGRTM
                     SVLLQYSRRNGAPIAGKALSVESSGVPWRHLGSGVTDAAGQLQIELRRDFLDEDADTA
                     PASFVITARIGLVSNSVTVTF"
     gene            complement(1523..2542)
                     /locus_tag="PKV97_08950"
     CDS             complement(1523..2542)
                     /locus_tag="PKV97_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06040.1"
                     /translation="MADNTEKPGKGKPTRTQAGATSPAAALPEGSILSAGALMPGAVG
                     GALALGADVSGELAELAPSFGDILASIGRGVADSQTALDRGLVDTATTLSQSNITVVT
                     DVVQELNDDGLPVPSATQLVTQNVSLINFVRPTAHQWSHVALRMDMTVGAVDNERGIS
                     VNSKTKSARATNVGLFFGFLGIGVSSFKEKSTFRSEESDFESNWAEGRVLMDALLQPR
                     NVDGFDPPAEVSIGPQIFMALGSVQETLNSGVVTARSTDIVITVRKANGAVNPTVTID
                     VDPGPFDLSFATADGFTGNTTNAAGQCRITLSRNIPSPLFQRRVRGTVTATLGQIERS
                     TTVSL"
     gene            complement(2564..>4318)
                     /locus_tag="PKV97_08955"
     CDS             complement(2564..>4318)
                     /locus_tag="PKV97_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB06041.1"
                     /translation="WSAFVRAPGADDGGADRGSGHGTHVAGLAAGDGGMSAGRLVGVA
                     PGARILMQAIEQYVDPLPEWRSRLQPGFYLAGRPLDLRELLEQARAAGAAIHADAWGV
                     EAAGAYTDDSWECDHFLRAHPEMLMIVAAGNAGRDRNRDGAVDAGTLYAPASAKSVLA
                     VGATEGPDAGIGLRGGWAALDPAGTRFPDPAQRGASVSGDAERLALLSSCGPTLDGRI
                     KPDLCAPGTNLAAARSTASSARGWGLANPLPWYQYLGGTSMSTGVMAGAAAVLRQRWR
                     QVLGEAPGGAALKALLIHGARAVHRRDGQGEEDRQRAGFGRLWLAGSLPGPDGPVLIE
                     DRVGVATGERRRIDCTGPGRLRAVLCWYDAPGEALINDLDLVLCDAGGSVLAWGNHPA
                     GAVGSPDRRNTVECIDVALGEGEWMLEVRGVNVPLGLQPWALATSPGVVVRATPAAPG
                     TPTVPADPLPAPRWRVDLPWTAVRGVGARRAIELERTLPGGLATLCAGVPDEDALAFL
                     PAALRAQIRSLAAFLGAPPPAAARLALAHPLHALLSGAPETASTVAWQALAAALSPLL
                     EALDRKVAREVRGERLFR"
     assembly_gap    4319..4418
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4419..4723)
                     /locus_tag="PKV97_08960"
     CDS             complement(<4419..4723)
                     /locus_tag="PKV97_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8/S53 subtilisin kexin sedolisin"
                     /protein_id="HNB06042.1"
                     /translation="MVSAPRAHPGVAPACFALVPAAATAGAVLARYGELALVEVERVP
                     VTAWASWTDLDPIRVHGVPRTLPSGCFEPGAGPRCILRFAGPVLPAWRETLATLGWTP
                     "
     gene            4970..5470
                     /locus_tag="PKV97_08965"
     CDS             4970..5470
                     /locus_tag="PKV97_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNB06043.1"
                     /translation="MSLAVRPSASSFTRSLLAVLASVLLAGCASLIQREPVRINVVGL
                     EPLPGAGMEMRFAVKLRVQNPNEAAIDFDGLALELDLDGKPFATGVSDRKGSVPRFGE
                     ALVEIPVSVSAIAVVRQALGVIEGEPKTEVPYTLRGRLAGGLLGGTRFSDAGMLKLPE
                     PLRNPR"
     gene            5557..6348
                     /locus_tag="PKV97_08970"
     CDS             5557..6348
                     /locus_tag="PKV97_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB06044.1"
                     /translation="MTLPTVHDARLQLDKRVATLTLARDDVRNALTGTALIDDIVGVA
                     EWVNRCEEVSVLVITGEGAAFSAGGNVKDMAARGGDFAGDVAEVATRYRQGIQRIPLA
                     LQAVEVPVIAAVNGPAIGAGFDLANMADIRIASTKAKFGETFLNLGIIPGDGGAWFMQ
                     RLIGYQQAFELTLSGRIVGAAEAKTLGIVLEVVEPEQLLPRAHELAARFAAQPPKALR
                     LTKRLMKMAQRMELKDFLDLSAAFQGMCHNEPEHLEAVQRLLART"
     gene            6384..>7297
                     /locus_tag="PKV97_08975"
     CDS             6384..>7297
                     /locus_tag="PKV97_08975"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNB06045.1"
                     /translation="MNGHAPHFDVEDFADPAAALARVHEIYDLAVEHLRRGLQHYVDG
                     ADIGRHVRACYPLLRVRTDTVARADSRLSYGFVAGPGVFETTLTRPDLFADYYLEQFR
                     LLVQNHGVALQVGSSTQPIPVHFALPEHDYLEGHLGPERRRLLRDHFDLPDLGAMDDG
                     IANGTFEPGPGEPHPLALFTAPRVDYSLHRLRHYTGTRPAFFQNFVLFTNYQFYIDEF
                     IRLGHELMADTASGHGYEAFVEPGNVVTRRADLPPQAEDADGTPPPRLPQMPAYHLVR
                     GDHAGITMVNIGVGPANAKTITDHIAVLR"
BASE COUNT         1064 a         2654 c         2500 g          979 t
ORIGIN      
        1 tgccctccag attcaccagg ctggacgtgc cgacgggctt caggttcatc gtgtcgacga
       61 gccgggggtc gagcagcgag ccggcggcgg tgcggaggtc cttgggatcg atgggcatgg
      121 cggatcgacg ggttggtgag gagggaagcg ggcgattcag gcggatggcg gcgagacgtc
      181 gggcatgccc tcgcgcggag ccaggcgcag ccgcaccatc aggctgctcg cggcctggta
      241 gccatacagg ctgacctggc gcggatccac cgggcgcgac atgaggcgcg gcgggacgct
      301 cccgctcgcg cgctcgaccg atgcggagag cgccacctcg agcgtcacct cggtgaaggt
      361 gtaccacagc ggcggcaccc ggggggcgtc ctcgtcgccg cgctcgatcg cgtcgaggcg
      421 agcggtggcg tggcggtcga gcgcctcctg cgccgcggcg accgagacta cggtgcgcgc
      481 gagcagttcg cccagctcga cggcctgctg gccggccagt tcgccggcac tgacgttcgc
      541 cttgtccatg cgttctcccg ccggcgcgga ggcgggcctt cccgcccccg cgccggaccg
      601 ttcagaaggt gacggtgacc gagttgctca ccaggccgat gcgcgcggtg atcacgaagc
      661 tggccggcgc ggtgtcggcg tcctcgtcga ggaagtcacg cctcagctcg atctggagct
      721 gccccgcggc atcggtgacg ccgctgccga gatgccgcca gggcacgccg gagctctcga
      781 ccgacagcgc cttgccggcg atcggcgcgc cattgcggcg actgtactgc agcagcacgc
      841 tcatggtgcg tccgaccaga tcgccgccat ccatgacgtc ggcgatctcg ccctggatga
      901 gcgcgagccg cggcccgcgg atcacctggt tgggcttggg gatgccgacg tcgtcgcgcg
      961 gctcgagcag cgcactggcg cggatgcggc cgagcgagac gtcggtggac acgtcggtcg
     1021 tggtggtgct cgacccggat ccgctcccgc tggtgaccat gccgggaccg aagaacatcg
     1081 acacgccgct gccgaagagc ccgaaattcg agcccgagta cgcgctcgtg gtctccgagg
     1141 acgcggcgaa ctcgcgcgcg tagaactcgc cctgcaggcg cacgctggtc cattggtaga
     1201 acaccggatc gatgaagctg atcagcggca gcgggcgcac gtggttggtg acatggtcga
     1261 gcgtgccgtc gtcgcggaac acctgctcgc gcaccgcgat cacgttgacc agcgtggcgg
     1321 ccagcgccga ggccgtcgcc gcgctcgagg tgtcgagctg cagctgggtc gccgccaccg
     1381 ccgcgccggt gcgttcgacc agcgtgccga aggcgatgcc ggcaccgcgc agttcgtcgg
     1441 agacgttggc gtcctcggcc agcgctcggg caaggggagg aagtagatca ggcatgatgg
     1501 tttctcctgt ggcgggcggc gctcagagcg acaccgtcgt gctgcgttcg atctggccca
     1561 gcgtggcggt caccgtgccg cgcacgcggc gctggaagag cgggctgggg atgttgcgcg
     1621 acagcgtgat ccggcactgc ccggccgcgt tcgtggtgtt gccggtgaag ccgtcggcgg
     1681 tcgcgaagga caggtcgaag gggccgggat cgacgtcgat cgtcacggtc ggattcaccg
     1741 cgccgttggc cttgcgcacc gtgatcacga tgtcggtgga gcgcgcggtg acgacgcccg
     1801 agttcagcgt ctcctgcacg ctgcccagcg ccatgaagat ctgcggtccg atggagacct
     1861 cggccggcgg gtcgaatccg tcgacgttgc gcggctgcag cagggcgtcc atcagcacgc
     1921 gcccctccgc ccagttcgat tcgaagtccg actcctcgga acggaaggtc gatttctcct
     1981 tgaacgacga cacgccgatg cccaggaagc cgaagaagag gccgacattg gtcgcccgcg
     2041 cgctcttggt cttgctgttg acgctgatgc cgcgctcgtt gtcgaccgca ccgacggtca
     2101 tgtccatgcg cagcgcgacg tggctccact gatgcgcggt gggacggacg aagttgatca
     2161 gcgacacgtt ctgggtcacg agctgggtcg cgctggggac cgggaggccg tcgtcgttca
     2221 actcctgcac gacgtcggtg accaccgtga tgttcgactg cgacagggtg gtcgcggtgt
     2281 cgaccaggcc acgatcgagg gcggtctgcg aatcggcgac cccgcggccg atcgaggcca
     2341 ggatatcgcc gaagctgggc gcgagctcgg cgagttcgcc ggagacgtcg gccccgaggg
     2401 cgagcgcgcc gccaaccgca ccgggcatca gcgcacccgc cgagaggatg ctgccctcgg
     2461 gcagcgcggc agccggcgac gtcgcgccgg cctgggtccg ggtcggcttg cccttgcccg
     2521 gtttttcggt gttgtcagcc atgatgcact ccttgtgagt ggttcatcgg aacaagcgct
     2581 cgccgcggac ctcgcgggcg accttgcggt cgagcgcttc gagcaaggga gagagcgcgg
     2641 cggccagggc ctgccacgcg acggtggagg cggtctcggg cgcgccggag agcagggcgt
     2701 ggagcggatg cgccagcgcg agccgcgccg cggcgggtgg cggcgcaccg aggaaggcgg
     2761 ccagactgcg gatctgcgcg cgcaaggccg cgggcaggaa ggccagcgca tcctcgtccg
     2821 gaacaccggc gcatagtgtg gcgagcccgc cgggcagcgt gcgctcgagt tcgatcgcgc
     2881 gtctggcccc cacgccgcgc accgccgtcc acggcaggtc gacccgccaa cgcggcgcgg
     2941 gcagcggatc cgcgggcacc gtcggcgtcc ccggcgccgc gggggtggcc cgcacgacga
     3001 cgccgggact cgtcgccagc gcccacggct gcagcccgag cggcacattc acgccgcgca
     3061 cctccagcat ccactcgccc tcgccgagcg ccacgtcgat gcattcgacg gtgttgcgcc
     3121 ggtccggact gccgaccgcg ccggccgggt ggttgcccca ggcaagcacg ctgccgccgg
     3181 catcgcacag caccaggtcg aggtcgttga tcagggcctc gcccggcgcg tcgtaccagc
     3241 acagcaccgc gcgcagccgt cccggccccg tgcagtcgat gcggcggcgc tcgccggttg
     3301 ccacaccgac gcggtcctcg atcagcaccg gcccgtcggg accgggcagg ctgccggcca
     3361 gccacaggcg accgaagccc gcgcgctggc ggtcctcctc gccctgtccg tcgcgccgat
     3421 gcaccgcccg tgcgccgtgg atcagcagcg ccttgagcgc ggcgccgccc ggtgcctcgc
     3481 cgagcacctg ccgccagcgc tgtcgcagca ccgccgccgc ccccgccatc acccctgtcg
     3541 acatcgaggt gccgccgaga tactggtacc agggcagcgg attggccagg ccccagcccc
     3601 gtgccgaact cgccgtgctg cgcgcggcgg cgaggttggt gccgggcgca cacaggtcgg
     3661 gcttgatgcg gccgtccagc gtgggaccgc agctcgacag cagcgcgagc cgctcggcgt
     3721 cgcccgagac ggacgcgccg cgctgtgccg gatcgggaaa gcgcgtgcca gcgggatcga
     3781 gcgccgccca cccgccgcgc aggccgatgc cggcatccgg tccctcggtg gcaccgacgg
     3841 caagaacgct tttcgcgctt gccggcgcgt acagcgtgcc ggcatcgacg gccccgtcgc
     3901 ggttgcggtc gcggccggcg ttgccggcgg cgacgatcat cagcatctcg ggatgggcgc
     3961 ggaggaaatg gtcgcactcc cagctgtcgt cggtataggc acccgcggcc tccacacccc
     4021 aggcgtcggc gtggatcgcc gcgccggcgg cgcgcgcctg ttcgagcagc tcgcgcaggt
     4081 cgagcggccg cccggcgagg tagaagccgg gctgcagccg gctgcgccat tccggcagcg
     4141 ggtcgacgta ctgctcgatc gcctgcatga ggatgcgcgc acccggcgcc acgcccacca
     4201 gcctgccggc gctcatgccg ccgtcgcccg cggccagccc cgccacatgg gtgccatggc
     4261 cgctgccacg atcggcgcca ccgtcgtccg cgcccggagc gcgcacgaag gccgaccann
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg cgtccagccc agcgtggcga
     4441 gcgtctcgcg ccaggctggc aataccgggc cggcgaagcg caggatgcag cgcggccccg
     4501 ctcccggctc gaagcatccg gacggaagag tgcggggcac gccatggacc cggatcggat
     4561 cgagatccgt ccagctcgcc caggccgtga ccggcacacg ttcgacctcg acgagcgcca
     4621 gctcgccata gcgagccaac accgcgcctg ctgtcgccgc ggccggcacg agggcgaaac
     4681 acgcgggcgc gacgccagga tgcgcgcgcg gggcggaaac catgctcgac tccagggaag
     4741 aaggctgcgc ggacaaccgg ggtgacgagc accggcgctc aaccgaccgg caccgcagtc
     4801 accaccgatg ctttcaccat agccccaatg cgggtcgaat tcaaaaggcg gagacgcttt
     4861 gtcccacgcc gatcgactgc ggcagcttcc ttgcacggcg cggttagaat gaacgccaca
     4921 ctcgcccacc gtggccgcag agcgcgcgcc cttgcccgga gtcggcacca tgtcccttgc
     4981 tgtccgcccg tctgcctcgt ccttcacccg cagcctgctc gccgtgctcg catcggtgct
     5041 gctcgccggc tgcgccagcc tgatccagcg cgagccggtg cgcatcaacg tcgtggggct
     5101 cgaacccctg ccgggcgcgg gcatggagat gcgcttcgca gtgaagctgc gcgtgcagaa
     5161 ccccaacgag gcggcgatcg atttcgacgg cctcgccctc gagctcgacc tcgacggcaa
     5221 gcccttcgcc accggcgtca gcgaccgcaa gggcagcgtg ccgcgcttcg gcgaggcgct
     5281 cgtcgagatc ccggtgtcgg tctcggcgat cgcggtggtg cgccaggcgc tcggcgtcat
     5341 cgaaggcgaa ccgaagaccg aggtgcccta caccctgcgc gggcggctcg ccggcgggct
     5401 gctcggcggc acccgcttca gcgatgcggg catgctgaaa ttgcccgagc cgctcaggaa
     5461 tccgcgctga acgcagcacc cgaaccagtc ccaggccctg ccccaggccg cgcaccgcgg
     5521 cccccaacta tccgccgccc gcaaggagac cgcacgatga ccctgcccac cgtccacgac
     5581 gcccgcctgc agctcgacaa gcgcgtcgcc accctcacgc tggcgcgcga cgacgtgcgc
     5641 aacgcgctca ccggcaccgc gctgatcgac gacatcgtcg gcgtcgccga atgggtgaac
     5701 cgctgcgagg aggtctccgt cctcgtcatc accggcgaag gcgcggcctt ctccgcgggc
     5761 ggcaacgtca aggacatggc ggcgcgcggc ggcgacttcg ccggcgacgt ggccgaggtc
     5821 gccacccgct accgccaggg catccagcgc atcccgctgg cgctgcaggc ggtcgaggtg
     5881 ccggtcatcg ccgcggtcaa cggcccggcg atcggcgccg gcttcgacct cgccaacatg
     5941 gccgacatcc gcatcgcatc gaccaaggcg aagttcggcg agaccttcct caacctcggc
     6001 atcatccccg gcgacggcgg cgcctggttc atgcagcgcc tgatcggcta ccagcaggcc
     6061 ttcgaactca cgctctcggg ccgcatcgtc ggcgccgcgg aggcgaagac gctcggcatc
     6121 gtgctcgagg tggtcgaacc cgagcagttg ctgccccgcg cgcacgaact ggccgcacgc
     6181 ttcgccgccc agcctcccaa ggccctgcgc ctcaccaagc gcctgatgaa gatggcgcag
     6241 cgcatggaac tgaaggactt cctcgacctt tccgccgcct tccagggcat gtgccacaac
     6301 gaacccgagc acctcgaggc ggtccagcgc ctgctcgcgc gcacctgaac cacgagcacc
     6361 tgaaccacga gcacccgcaa acgatgaacg gccacgcccc ccacttcgac gtcgaggact
     6421 tcgccgatcc ggccgcggcg ctggcccggg tgcacgagat ctacgacctc gcggtcgagc
     6481 acctgcgccg cggcctgcag cactacgtcg acggcgccga catcggccgc cacgtgcgcg
     6541 cctgctatcc gctgctgcgc gtgcgcaccg acaccgtggc gcgcgccgat tcgcggctgt
     6601 cctacggctt cgtcgccggg ccgggcgtat tcgagaccac gctgacccgg cccgacctgt
     6661 tcgccgacta ctacctggag caattccgcc tgctggtgca gaaccacggc gtggccttgc
     6721 aggtgggcag cagcacgcag cccatcccgg tgcatttcgc cctgcccgag cacgactacc
     6781 tggaaggcca cctcggcccc gagcgccgcc gcctgctgcg cgatcacttc gacctgcccg
     6841 acttgggcgc catggacgac ggcatcgcca acggcacctt cgagcccggc ccgggcgagc
     6901 cgcacccgct cgcgctgttc accgcgccgc gggtggacta ctcgctgcac cgcctgcgcc
     6961 actacaccgg cacgcggccg gccttcttcc agaacttcgt gctgttcacc aactaccagt
     7021 tctacatcga cgagttcatc cgcctcggcc acgagctcat ggccgacacc gcctccggcc
     7081 acggctacga ggccttcgtc gagccgggca acgtggtcac ccgccgcgcc gaccttccgc
     7141 cgcaggccga ggacgccgat ggcaccccgc cgccgcgcct gccgcagatg ccggcctacc
     7201 acctggtgcg cggcgaccac gccggcatca cgatggtgaa tatcggcgtc ggcccggcca
     7261 acgccaagac catcaccgac cacatcgcgg tgctgcg
//
LOCUS       DAOQNN010000141         7274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5414_length_7274_cov_12.302535, whole genome shotgun sequence.
ACCESSION   DAOQNN010000141 DAOQNN010000000
VERSION     DAOQNN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5414_length_7274_cov_12.302535"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..519
                     /locus_tag="PKV97_08980"
     CDS             100..519
                     /locus_tag="PKV97_08980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNB06046.1"
                     /translation="MDCPLCALPSELVLWEDERCRVIRVDDAAHPGFCRVVWGEHVGE
                     MTDLSPADRRHLLDVVMATEAALRNLLDPDKINLASFGNMVPHLHWHVIPRYRDDRHF
                     PESVWGAPQRDGVIHPAADAVALARAIDGALHPSGPD"
     gene            534..2153
                     /locus_tag="PKV97_08985"
     CDS             534..2153
                     /locus_tag="PKV97_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06047.1"
                     /translation="MDYSQPAVCLALLQKLHPTDFRATHASLGRIVNGLLEASPAPNQ
                     HLEVLEAARETLAFVQGEMAHAYGIHPQPPARNQEGGALERVVSLWKAVARSYARIAD
                     ADAREGTLEDQRALLAQRRVFYEGLVLVEYYRAHLELPQGLWRAVHVAYNDAVRNGLA
                     QVRVTDPLNEVWRAQSAQEAYVATLLAELANPYGRGERELNWVVRWAQRFAPYCTLDT
                     GIEGHKPSTYGIDTDGDSGLRPLGLLARTPTVLRFDGSGLATQIQAVLAQFKRGVTPA
                     SLGLGNDCSASSSARLLLSLYRPWGLAASGRRFPRRQKAGAIDFTGDWLAIGYAIKGE
                     LFQQPVGEQRARAFRDDISLLTFGERVATVDGSTSESRRREAQAADLGMECERWELMD
                     QSVGGFRLRRSGNGARLDHHQLVGIRPPDADQLLIADLSWLMYRTDGTLEVGVNVLPG
                     VPRVVGVRRTAARTGAREPFHQAFVLPPSPALKTAGALVLPPAWFKKDRIVEVRDGEE
                     RQQVRLLKVLMRGANFDQCSFVPVEQEVRPA"
     gene            complement(2140..3456)
                     /gene="phoR"
                     /locus_tag="PKV97_08990"
     CDS             complement(2140..3456)
                     /gene="phoR"
                     /locus_tag="PKV97_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HNB06048.1"
                     /translation="MIRRPLSYVALVIVLPLGTLMVLAALAAAYRGSDAALALLVVGL
                     VGWTIMLVHNLRQLVIWARQPVGTPVPQVEGLWGRIFAEIGDRMQSECEVKERLSAEL
                     GRFQQAALAMPDGVIYLAEDDTIEWMNPMAEQHFDLDRRKDLRMALPGLVRQPEFVRY
                     LGAREYAEPLVMRSLRRRERSLQVQVIGFAGNRRMVLSRDVSQLERLETMRRDFVSNV
                     SHELRTPLTVVCGFLETLIDGLDDFGRDEVLQYLSLASEQSSRMQRLIEELLTLSALE
                     TGAPAPAEEQVDAQALVRDVVRDCELLSAGRHEVRLVLEGGGELLGSHKELRSAFSNL
                     ASNAVRYTPAGGHIEIGWRSGAGGGEFWVADDGIGIDSAHIPRLTERFYRVDRGRSRE
                     TGGTGLGLAIVKHVTTRHQGELAIESEPGRGSRFTLRFTPARVRPA"
     gene            complement(3491..4201)
                     /gene="phoB"
                     /locus_tag="PKV97_08995"
     CDS             complement(3491..4201)
                     /gene="phoB"
                     /locus_tag="PKV97_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HNB06049.1"
                     /translation="MPANILLVEDEPAIQELIAANLARAGHHVVRANDAESAQRIVRE
                     ALPDLVLLDWMLPGASGVEFARRLRADERTRDIPIIMLTARGEEQDKVTALEVGADDY
                     VTKPFSPRELVARIKAVLRRRAPQTTEDVVELGGLRLDPATHRVSAGETALALGPTEF
                     RLLHFLMTHPERVHSRAQLLDQVWGDHVFVEERTVDVHIRRLRCALEPSAHDALIQTV
                     RGSGYRFSVHTAQAAAAS"
     gene            complement(4434..5408)
                     /gene="hcrB"
                     /locus_tag="PKV97_09000"
     CDS             complement(4434..5408)
                     /gene="hcrB"
                     /locus_tag="PKV97_09000"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit beta"
                     /protein_id="HNB06050.1"
                     /translation="MNILADFRTHRPATLADAVSALAADGAVPLAAGTDLLPNLRRGL
                     GQPAVLVDLTGIGGLETISILADGSLRIGAGATLEAIAEHGLVRNAWPALAEAAAAVA
                     GPTHRAAATLGGNLCQDTRCIFYNQSEWWRAGNGYCLKYQGDKCHVIVKNDRCYATYH
                     GDVAPALMVLGARVEIAGPAGRRSLAVEELFREDGAGHLSLGRGELVVAVEVPPASGW
                     RTVYAKARIRDAVDFPLAGVAVALRRDGDRVAGLKVAITGTNSAPLRVPTDELVGGRW
                     DAAAAETLSQLVRKTSNVLKTTVTGVGYRRRVLLALAPRTVDALWQGG"
     gene            complement(5405..>7274)
                     /gene="hcrA"
                     /locus_tag="PKV97_09005"
     CDS             complement(5405..>7274)
                     /gene="hcrA"
                     /locus_tag="PKV97_09005"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit alpha"
                     /protein_id="HNB06051.1"
                     /translation="HDDKPDNVLREVHAEFGDVAAAFAEADLIREKTYTFAGVNHVHM
                     ELNATLAEYDPVRDMLTLNTTTQVPYYVHLKVAACLQMDSARIRVIKPFLGGGFGART
                     ETLHFEIIAALLARKARGTVRLVQTREETFIAHRGRPWTEVKMKLGLKKDGKIAALAL
                     EATQAGGAYAGYGIITILYSGALMHGLYHIPAIKHDAWRVYTNLPPSGAMRGHGTVDT
                     RAAFEALLTEMAEELGIDSLRIRQINMLPEIPYVTMYAQRVMSYGVPECLEKVKAASG
                     WEERKGRLPKGRGLGIALSHFVSGTSTPKHWTGEPHATVNLKLDFDGGITLLTGAADI
                     GQGSNTMASQVAAEVLGVRLDRIRVISADSALTPKDNGSYSSRVTFMVGNASISAAEE
                     LKGILVKAAAKKLDAREEDIEVIDELFMVSGSQDPGLTFQEVVKAAMVDSGTITVKGT
                     FTCPTEFQGDKRIRGSAIGATMGFCYAAQVVEASVDEITGKVTAHKVWVAVDVGKALN
                     PLAVEGQTQGGVWMGMGQALSEEIVYDKGRMLHGNILDYRVPTMMESPDIEVIIVESL
                     DPNGPFGAKEASEGMLAGFLPAVQEAVYEAVGVRATDFPLSPDRITELLDAKEAAA"
BASE COUNT         1079 a         2539 c         2545 g         1111 t
ORIGIN      
        1 cgacaaggct ccgagtgcgt aacagttcct cgcccgcccg cagcgtcggg cctagattga
       61 aaatccgtcg aaccctccgc agccgaccga gaaaccctca tggattgccc cctgtgcgcc
      121 ctgccgtccg aactcgtcct ctgggaagac gagcgctgcc gcgtcatccg cgtcgatgac
      181 gccgcccacc cgggcttctg ccgggtggtg tggggcgagc acgtcggcga gatgaccgac
      241 ctctcgccgg ccgatcgccg tcatcttctg gatgtggtga tggccaccga ggcggcgctg
      301 cgcaatctgc tcgacccgga caagatcaac ctggccagct tcggcaacat ggtgccgcac
      361 ctgcactggc acgtgatccc gcgctaccgc gacgaccgcc acttccccga gtcggtgtgg
      421 ggcgcaccgc agcgcgacgg cgtgatccac cccgccgccg acgcggtcgc cctcgcacgc
      481 gcgatcgacg gcgcgctgca cccgagcgga ccggactgag gacgcgcgaa gccatggact
      541 acagccagcc cgcggtctgc ctcgccctgc tgcagaagct gcacccgacc gatttccgcg
      601 ccacgcatgc cagcctgggt cgcatcgtca acggcctgct ggaggcctcc ccggcgccca
      661 accagcacct cgaggtgctg gaggccgcgc gcgagacgct cgccttcgtg cagggcgaga
      721 tggcgcacgc ttatgggatc cacccgcaac ctccggcgcg caaccaggag ggcggtgcgc
      781 tcgagcgcgt ggtctcgctg tggaaggcgg tggcgcgctc ctacgcacgc atcgccgacg
      841 ccgacgcgcg cgaaggcacg ctcgaggacc agcgtgcgct gctcgcccag cgccgggtgt
      901 tctacgaggg cctcgtactg gtggagtatt accgcgccca cctcgagttg ccgcaaggtt
      961 tgtggcgggc ggtgcatgtc gcctacaacg atgcggtgcg caacggactc gcacaggtac
     1021 gcgtgaccga cccgctcaac gaggtctggc gtgcacagag tgcccaggaa gcctacgtcg
     1081 ccaccctgct cgccgagctc gccaacccct atggccgcgg cgagcgcgaa ctgaactggg
     1141 tcgtgcgctg ggcgcagcgc ttcgcgccct attgcacgct cgacaccggc atcgaaggac
     1201 acaagcccag cacctacggc atcgacaccg acggcgacag cggcctgcgc ccccttgggc
     1261 tgctcgcgcg cacgcctacc gtgctgcgct tcgacggcag cgggctcgcc acccagatcc
     1321 aggccgtcct cgcgcagttc aagcgtggtg tcacgcccgc ctcgctcggg ctcggcaacg
     1381 actgctcggc cagttccagc gcgcgcctgc tgctgtcgct ctaccgcccg tggggactgg
     1441 cggcttccgg gcggcgcttc ccgcgccggc agaaagccgg cgcgatcgac ttcaccggcg
     1501 actggctcgc gatcggctac gcgatcaagg gcgagctctt ccagcaaccc gtcggcgagc
     1561 aacgggcgcg cgccttccgc gatgacatct ccctgctcac cttcggcgag cgcgtggcca
     1621 cggtcgatgg cagcacctcg gagagccggc gacgcgaggc gcaggccgcg gacctgggca
     1681 tggagtgcga gcgctgggaa ctgatggacc agtcggtggg cggcttccgc ctgcgccgca
     1741 gcggcaacgg ggcgcgcctg gaccaccacc agctcgtcgg catccgcccg cccgacgccg
     1801 atcagctgct gatcgccgac ctgagctggt tgatgtaccg caccgacggc acgctggaag
     1861 tcggcgtcaa cgtgctgccc ggcgtaccgc gggtggtcgg ggtgcggcgc acggctgcac
     1921 gcacgggagc gcgcgaaccc ttccaccagg ccttcgtgct gccgccctcg cccgcgctca
     1981 agaccgcagg cgcgctcgtg ctgccgcccg cctggttcaa gaaggaccgc atcgtcgagg
     2041 tacgggacgg cgaggagcgt cagcaggtgc gcctgctcaa ggtgctgatg cgtggcgcga
     2101 acttcgacca gtgcagcttc gtgccggtcg agcaggaagt caggccggcc tgacccgtgc
     2161 cggcgtgaag cgcagcgtga agcggctgcc ccgccccggt tcgctctcga tggcgagttc
     2221 gccctggtgg cgcgtggtga cgtgcttgac gatcgccaac cccagccctg tgcccccggt
     2281 ctcgcgcgac cgcccgcgat ccacccggta aaagcgttcg gtcaggcgcg ggatgtgcgc
     2341 cgagtcgatg ccgatgccgt cgtccgcgac ccagaattcg ccaccacccg caccgctgcg
     2401 ccagccgatc tcgatgtggc cgccggccgg cgtgtagcgc accgcgttgc tggccaggtt
     2461 ggagaaggcg ctgcgcaatt ccttgtggct gccgaggagt tcgccgccac cctcgagcac
     2521 caggcgcacc tcgtgacggc ccgcggagag cagttcgcaa tcgcgcacga cgtcgcgcac
     2581 cagcgcctgg gcatccacct gctcttccgc cggcgcgggc gccccggtct cgagcgccga
     2641 caaggtcagc aactcctcga tcaggcgctg catgcgcgag gactgttcgc tggcgaggct
     2701 caggtactgc agcacctcgt cgcgcccgaa gtcgtcgagg ccgtcgatca gggtctcgag
     2761 gaagccgcag accacggtca gcggggtccg cagctcgtgc gacacgttgg agacgaagtc
     2821 gcgccgcatg gtctccaggc gctcgagctg ggagacgtcg cgcgacagca ccatgcgccg
     2881 gttgccagcg aagccgatga cctgcacctg caggctgcgc tcgcgccggc gcagcgagcg
     2941 catcaccagc ggttcggcat actcgcgcgc gccaaggtat cggacgaact cgggctggcg
     3001 caccaggccg ggcaaggcca tgcgcaggtc cttgcggcgg tcgaggtcga agtgctgctc
     3061 ggccatcgga ttcatccact cgatggtgtc gtcctcggcg aggtagatca cgccgtcggg
     3121 catggccagc gcggcttgct ggaagcggcc gagttcggcc gagaggcgct ccttgacctc
     3181 gcactcgctc tgcatgcggt cgccgatctc ggcgaagatc cgcccccaca ggccctcgac
     3241 ctgcggcacc ggggtgccca ccggctggcg cgcccagatg acgagctggc gcaggttgtg
     3301 caccagcatg atcgtccagc cgaccagccc gaccaccagc agggcgagtg cggcatcgct
     3361 gccgcggtag gcggcggcca gggccgccag gaccatcagc gtccccagcg gcaggacgat
     3421 gacgagggcg acgtagctga gcgggcggcg gatcactggg agcggatcac gggacggatc
     3481 gggggtgcgc tcaggaggcg gccgcggctt gcgcggtgtg caccgaaaaa cgatagccgc
     3541 tgccgcgcac ggtctggatc agcgcgtcgt gcgcgctggg ttcgagcgcg cagcgcaggc
     3601 ggcggatgtg cacgtccacc gtgcgctcct cgacgaagac atggtctccc cacacctggt
     3661 cgagcagctg cgcacgcgaa tgcacgcgct cgggatgggt catgaggaaa tgcagcaggc
     3721 ggaactcggt cggtccgagc gcgagcgcgg tctcgcccgc gctgacacgg tgggtggcgg
     3781 ggtccaggcg caggccgccg agctcgacca cgtcctcggt ggtctgcggc gcacgccggc
     3841 gcagcaccgc cttgatgcgc gcgaccagct cgcgcgggct gaagggcttg gtaacgtagt
     3901 cgtcggcacc gacctcgagc gcggtgacct tgtcctgctc ctcgccgcgc gcggtcagca
     3961 tgatgatcgg gatgtcgcgg gtgcgctcgt cggcacgcag ccggcgggcg aattcgacgc
     4021 ccgaggcgcc gggcagcatc cagtccagca gcaccagatc gggcagcgcc tcgcgcacga
     4081 tgcgttgcgc gctctcggcg tcgttggcgc gcaccacgtg gtggccggcg cgggcgaggt
     4141 tggccgcgat cagctcctgg atcgccggct cgtcttccac gagcaatatg ttcgctggca
     4201 tgcctgtgct ccctgaaaaa tgcccgtgaa gtgtattgca gctccttgaa gctttgatga
     4261 cacgtcatcg aagcgcaatg cacaatcccc gcgcgcctcc gtccaggccg gacaagaggc
     4321 gtgcggggcg ggcgaagcgc gccatcgtgg ggcgccacgc gggcaggatg cttgcggtcg
     4381 ccccctcgca gagggcgcgg cacagggcga acggaacacc aggctacaag gattcagcct
     4441 ccctgccaga gcgcgtcgac cgtccgcggc gccagcgcca gcagcacccg gcgccgatag
     4501 ccgacgccgg tgacggtggt cttgagcacg ttcgaggtct tgcgcaccag ctgggacagg
     4561 gtctcggcgg cggcggcatc ccagcgccct cccacgagct cgtccgtggg cacgcgcagc
     4621 ggggcggagt tggtgccggt aatcgccacc ttcaggccgg cgacccggtc tccgtcacgg
     4681 cgcagggcga cggcgacccc cgcgagcgga aaatcgacgg cgtcgcggat gcgtgccttg
     4741 gcgtagacgg tcctccagcc gctggcgggg ggaacctcca cggccacgac cagttcgccc
     4801 cgtccgaggc tcaggtgccc cgccccgtct tcgcggaaca attcctccac cgcgaggctg
     4861 cgcctgcccg cgggccccgc gatctcgaca cgcgcgccca gcaccatcag cgccggggcg
     4921 acgtcgccgt ggtaggtggc gtagcagcga tcgttcttga cgatgacgtg gcacttgtcg
     4981 ccctggtact tgaggcagta gccgttgccg gcgcgccacc actcgctctg gttgtagaag
     5041 atgcagcgcg tgtcctggca caggttgccg cccaaagtgg ccgcggcgcg gtgggtcgga
     5101 cccgcgaccg ccgcggccgc ctcggccagt gccggccagg cgttgcggac gagcccgtgc
     5161 tcggcgatgg cctccagcgt ggcgccggca ccgatgcgca ggctgccgtc ggcgaggatg
     5221 gagatcgtct ccaggccccc gattccggtc agatccacca gcacggcggg ctgccccagg
     5281 ccacggcgaa gattgggcag caggtcggtg ccggcagcca ggggaacggc cccgtcggcg
     5341 gccagcgcac tgaccgcgtc ggccagggtg gcggggcgat gggtgcggaa atcggcgagg
     5401 atgttcatgc tgcggcttcc ttggcgtcga gcagttcggt gatgcggtcg ggactgagcg
     5461 ggaaatcggt ggcgcgcacg ccgaccgcct cgtacaccgc ctcctggacg gcgggaagga
     5521 agccggccag catgccttcc gaggcttcct tggcgccgaa cgggccgttg ggatcgaggc
     5581 tctcgacgat gatcacctcg atgtcggggc tctccatcat ggtggggacc cggtaatcca
     5641 ggatgttgcc gtgcagcatc ctgcccttgt catagacgat ctcttccgac agcgcctggc
     5701 ccatgcccat ccacacgccg ccttgcgtct gcccttccac cgcgagcggg ttgagcgcct
     5761 tgcccacatc gaccgccacc cacaccttgt gcgcggtgac cttgccggtg atctcgtcca
     5821 ccgaggcctc caccacctgg gcggcgtagc agaagcccat ggtggcgccg atcgcgctgc
     5881 cgcggatgcg cttgtcgccc tggaactcgg tcgggcaggt gaaggtgccc ttcacggtga
     5941 tggtgccgga gtcgaccatc gcggccttga ccacctcctg gaaagtcagc ccggggtcct
     6001 ggctgcccga gaccatgaac aactcgtcga tgacctcgat gtcctcctcg cgggcgtcga
     6061 gcttcttcgc cgccgccttg acgaggatgc ccttgagctc ctcggcggcg gaaatcgacg
     6121 cgttgccgac catgaaggtg acgcgggagg aatacgagcc gttgtccttg ggcgtcagcg
     6181 cgctgtcggc ggagatcacc cggatacggt cgaggcgcac gcccaggacc tcggcggcca
     6241 cctgcgaggc catggtgttg gagccctggc cgatgtcggc ggcgccggtc agcagggtga
     6301 tgccgccgtc gaagtcgagc ttgaggttga ccgtggcgtg gggttcgccc gtccagtgct
     6361 tgggcgtcga agtccccgac acgaaatgcg acagcgcgat gccgaggccg cgccccttgg
     6421 gcagcctgcc cttgcgttcc tcccagcccg aggcggcctt gaccttctcg aggcactccg
     6481 gcacgccata gctcatcacc cgctgggcgt acatggtgac gtaggggatc tccggcagca
     6541 tgttgatctg gcggatgcgc agcgagtcga tgccgagctc ctcggccatc tcggtcagca
     6601 gcgcctcgaa ggcggcgcgg gtgtccacgg tgccgtggcc gcgcatcgcg ccgctcggcg
     6661 gcaggttggt gtacacgcgc caggcgtcgt gcttgatcgc cgggatgtgg tagagcccgt
     6721 gcatcagcgc gccgctgtag aggatggtga tgatgccgta gccggcatac gcaccgccgg
     6781 cctgggtggc ctcgagcgca agggcggcga tcttgccgtc tttcttcagg ccgagcttca
     6841 tcttcacctc ggtccacggt cgcccgcgat gcgcgatgaa ggtctcctcg cgggtctgca
     6901 ccaggcgcac cgtgccccgg gccttgcgcg cgagcagcgc ggcgatgatc tcgaagtgca
     6961 gggtctcggt gcgggcgccg aagcctccgc ccaggaaggg cttgatcacg cggatgcggg
     7021 ccgagtccat ctgcaggcag gccgccacct tcaggtgaac gtagtagggc acctgggtgg
     7081 tggtgttcag cgtcagcatg tcgcgcaccg ggtcgtactc ggccagggtg gcgttgagct
     7141 ccatgtggac gtggttgacc ccggcgaagg tgtaggtctt ctcgcggatc aggtcggcct
     7201 cggcgaaggc cgcggcgacg tcgccgaact cggcgtgcac ctcgcgcagc acgttgtcgg
     7261 gcttgtcgtc gtgg
//
LOCUS       DAOQNN010000142         7274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5415_length_7274_cov_7.591633, whole genome shotgun sequence.
ACCESSION   DAOQNN010000142 DAOQNN010000000
VERSION     DAOQNN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5415_length_7274_cov_7.591633"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1474)
                     /locus_tag="PKV97_09010"
     CDS             complement(<1..1474)
                     /locus_tag="PKV97_09010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNB06052.1"
                     /translation="MIRDPATPTLFQRRVYGTVGLTLLVGLYLLVGLTAHDPWRGDDA
                     RHFGPVLAMLRGESWLFPLLAGEPLVEHPPLYYWVASAFSALTAAFLPAHAGARLASA
                     VFVALAIWWLARAAESLHGRAARTPAALLALGTLGLVLHAHETQPLLALFAMLALTLS
                     GLAELPTRPLAGGLKAGMGSGLAFLSAGFGGLWMSAPLLPLAMLLSADCRSPRASGAV
                     LLGLCLALTTAALWPLVLHLQHPELLSLWLRNEWQWIVGPMLRPAELPRLLELGAWFM
                     WPLWPLALWALWRARRQPGVLAILLPLASAGLVLAWLVAIDDLSQARLIPLVPALALL
                     AAGGVASLRRGAASALDWFSLMSLAVFGILVWLAWSAQIAGWPSGLARHVARNAPGFM
                     PPDANLRLLAGVAISVAWILLVARLPRSPARAPANWAIGLVMLWSLAVVLLMPWFDQG
                     RSYRAVVESLDIAVRGERGAQASECIATTGLPDAMKLSMDY"
     gene            complement(1657..1875)
                     /gene="rpmE"
                     /locus_tag="PKV97_09015"
     CDS             complement(1657..1875)
                     /gene="rpmE"
                     /locus_tag="PKV97_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNB06053.1"
                     /translation="MKADIHPKYADVNVTCSCGNTFVTRSTLGKPQYHVEVCSACHPF
                     YTGKQKIVDTAGRVERFRQKYGNVQRAS"
     gene            2057..3067
                     /locus_tag="PKV97_09020"
     CDS             2057..3067
                     /locus_tag="PKV97_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNB06054.1"
                     /translation="MRLLLAPMEGVADFVLRDVLTAIGGYDLAVSEFVRVSGTLLPRR
                     MFERICPEILAGSRTAAGTPVAVQLLGSDPVLLAENAARLAALSPHGIDLNFGCPAKV
                     VNRHGGGAMLLDRPRLLHDIAAAVRAAVPAAIPVSAKMRLGIADPSRALDCAQALADG
                     GAQAIVVHARTREQGYRAPAHWEWVARIGDAVRVPVTANGEVWSVRDWARCREVSAAE
                     DVMIGRGAVSDPWLARRIRGERGTEPDERDWVELHPHLLRYWEGVQGRAAPVHACGRL
                     KLWLGMLRRNYPAAGRLHEAVRRVVHPSRMNEELRRHGIPACDHPPSARADGPRPTSP
                     DR"
     gene            3078..3755
                     /locus_tag="PKV97_09025"
     CDS             3078..3755
                     /locus_tag="PKV97_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB06055.1"
                     /translation="MSYANSRIPQSAQQGVHEALAERVRKHLEHPFRKPCTDYNRAAL
                     AEALAGWGRSAPLILDAGCGVGHSTIQIARQFPDHWVIGVDQSADRLARRKPYPEALL
                     PKNMVFVRADLVDFWRLLDEAGLRLARHYLLYPNPWPKIGHLGRRWHAHPVFPWIPRL
                     GGVLECRSNWKVYIEEFAFALGLVLGREVAWEGFEAVTPLTPFERKYRDSGQPLYRIG
                     LDLAAAG"
     gene            3834..4598
                     /locus_tag="PKV97_09030"
     CDS             3834..4598
                     /locus_tag="PKV97_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNB06056.1"
                     /translation="MEQRGADASALRTMSDEDFEALEEILTSDIVPEDCMDLEMLDGY
                     LAGVMISPRPIPAERWMPGVWSAHGDEVGFGSGSGLQRAIRLVKAYHNELLATLGLDD
                     EDEACWEPFCFAVAEGDGLKLGEAWIDGFAQGLDLWPEGWEEGLDAEAVEAVRETLDE
                     VLAPWGEEDAAKADDETRLGWLADVGEAVNDIFAHWRDLGLPAAEPLSTEAPRKPASA
                     GPGRNDPCPCGSGKKYKKCCGANGAEPEPESGEAET"
     gene            4936..5295
                     /locus_tag="PKV97_09035"
     CDS             4936..5295
                     /locus_tag="PKV97_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNB06057.1"
                     /translation="MDEFNPCLDCGICCTHFRISFYWAEADDAPGGFVPAEMTEKLNH
                     TLRCMKGSNQVPRRCSALSGEVGRQVACSIYENRPTPCREFPVYFEDGTPNPKCDELR
                     ATIGLPPLPQSPLPRAA"
     gene            complement(5317..6303)
                     /locus_tag="PKV97_09040"
     CDS             complement(5317..6303)
                     /locus_tag="PKV97_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HNB06058.1"
                     /translation="MTSTPTARDPASDAAPASASAPAHRSPSDAAALTAHPPSSARSR
                     ADDLLERTDHVIAAALASGALQPIRTELDWLEQDGLRFSVRWVSSLALKDRARVDTVT
                     ARRPDFNPFLPPEPALTVAALGADHLVVLNKFPVIDRHLLIVTRRFEDQSTPLGGADF
                     AALAAVVGAHGGLGFYNGGRIAGASQAHKHLQWVPAQASALEDFLPPLRPGAAAENLA
                     LPWAHAAVGMDESAWSHPDDAGSVLLAAYRLACERLELPCDADPMPPYNLLVSRGALL
                     LVPRTREKWDDVSLNSLAWAGSLFVRDRAQIDGLRRHGPLEVLAAVSRPRGG"
     gene            6383..6607
                     /locus_tag="PKV97_09045"
     CDS             6383..6607
                     /locus_tag="PKV97_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNB06059.1"
                     /translation="MAIRFEVRGEYIQLDQLLKAAGLVESGGAAHAAVENGLVKVDGK
                     PESRKRAKLRPGQRVSFAGEAVELVEEGAG"
     gene            complement(6684..>7274)
                     /locus_tag="PKV97_09050"
     CDS             complement(6684..>7274)
                     /locus_tag="PKV97_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HNB06060.1"
                     /translation="ILRQNHESNRALFEAFESDITVVPSRVIAHECIAILSTPLLAPF
                     LAEIRRRDEEWCGALLHRLTRHLGWKVPRIRSQRVNLSSAPALYRRLMRGETITLERL
                     LRSPADRSMALDCAVLYLERDDDDHRMTPAADEKLRPGDELLFAGTRRALEDVALIFA
                     NEHTLEYILTGRDLPGGRVWEMLAQRKHGKRSPQLP"
BASE COUNT         1098 a         2489 c         2695 g          992 t
ORIGIN      
        1 aatagtccat cgacagcttc atcgcgtcgg gcagcccggt ggtggcgatg cattcgcttg
       61 cctgcgcgcc gcgttcgccg cgcactgcga tgtcgagcga ttccaccacc gcgcggtagc
      121 tgcgcccctg gtcgaaccag ggcatcagca gcactaccgc caggctccac agcatcacca
      181 ggccgatcgc ccagttcgcc ggcgcacgcg cgggcgaacg cggcaggcgg gcgacgagga
      241 ggatccaagc cacgctgatg gcgacgcctg caagcaggcg caggttggcg tcgggcggca
      301 tgaagccggg ggcgttgcgc gcgacgtgac gtgcgagacc gctcggccag ccggcgattt
      361 gggcgctcca tgccagccag acgaggatgc cgaacactgc caggctcatc agcgagaacc
      421 agtcgagcgc gctcgcggcg ccgcgccgca ggctggccac gccgcccgca gcgagcaagg
      481 cgagcgcggg caccagcggg atcaaccggg cctgcgagag atcgtcgatc gccaccagcc
      541 aggccagcac cagtccggcg ctcgccagcg gcagcaggat cgcgagcacg ccgggctggc
      601 ggcgcgcgcg ccagagcgcc cacaaggcca gcggccacag cggccacatg aaccaggcgc
      661 cgagctcgag cagtcgcggc agctcggccg ggcgcagcat cgggccgacg atccactgcc
      721 actcgttgcg cagccacagg ctcaggagtt cggggtgttg caggtggagc acgagcggcc
      781 acagcgcggc ggtggtcagc gcgaggcaca gtcccagcag cacggcgccg ctcgcgcgcg
      841 ggctgcggca gtcggcgctc agcagcatcg ccagcggcaa caggggagcg ctcatccaca
      901 ggccgccgaa gcccgcggag aggaaggcca ggccgctacc catgcccgcc ttcaggcccc
      961 cggcgagcgg gcgtgtcggc aactccgcga ggccggacag ggtcagtgcg agcatggcga
     1021 agagggcgag caggggctgc gtctcgtgcg catgcaggac caggcctagc gtgccgagcg
     1081 cgagcagcgc ggcgggcgtg cgggcggcgc gaccatgcag ggattcggcc gcgcgcgcca
     1141 gccaccagat cgccagcgcc acgaagacgg cgctcgccag gcgggccccg gcgtgcgccg
     1201 gaagaaaggc cgcggtgagc gcggagaagg ccgaggcgac ccagtaatac agcgggggat
     1261 gctccaccag gggttcgccg gcgagcaggg gaaacagcca ggactcgccg cgcagcatcg
     1321 ccagcacggg cccgaaatgg cgcgcgtcgt cgccgcgcca ggggtcgtgc gcggtcaggc
     1381 cgacgagcag gtacaggccg accagcaggg tcaggccgac cgtgccgtag acgcggcgct
     1441 ggaacagggt gggcgtggcg ggatcgcgga tcatgcgggc attgtgcttc ggagtggctg
     1501 cgcggaccaa gcgcggcgga gtgggcagga cggagagggc gcgggtgcct gccggcggcg
     1561 gggcaataaa aaaggcagct tgcgctgcct tcctcggtgc ctgcggaatc ctgccacgac
     1621 cgcccggggc ggtgcggatc ccggctttgc gacggctcag ctcgcgcgct ggacgttgcc
     1681 gtacttctgg cggaagcgct cgacgcggcc ggcggtgtcg acgatctttt gcttgccggt
     1741 gtagaacgga tggcaggccg agcacacctc gacgtggtac tggggcttgc ccagggtcga
     1801 acgggtcacg aaggtgttgc cgcacgagca ggtgacgttg acgtcggcgt acttgggatg
     1861 gatgtccgct ttcatggtga tttcctgaat cttgcgggcg gaggttgtga ccgccctgtg
     1921 tcgtaaagcg cgagattatc cgccagccgc cccgtcctga caaggaaaaa cacgtctgct
     1981 gtcgtgcgcc gggacggttt ttcgggcggc gaccggtaca atcgcgcgcc tgatcaatca
     2041 ccgacgaggt tcctgcgtgc gcctgctgct cgcgcccatg gagggcgtgg ccgatttcgt
     2101 gcttcgcgac gtgctgaccg cgatcggcgg ctacgacctc gcggtgtcgg aattcgtccg
     2161 cgtctccggc accctgctgc cgcggcgcat gttcgagcgc atctgcccgg agatcctcgc
     2221 cggcagccgc accgccgcgg gcacgccggt ggcggtgcaa ctgctcggca gcgatcccgt
     2281 cctgctcgcg gagaacgcgg cgcgcctggc cgcgctgagc ccgcacggca tcgacctcaa
     2341 cttcggctgc ccggccaagg tggtcaatcg ccatggtggc ggtgcgatgc tgctcgaccg
     2401 cccgcgcctg ctgcacgaca tcgccgccgc agtgcgtgcc gccgtgcccg cagcgattcc
     2461 ggtgagcgcc aagatgcgcc tgggcatcgc cgacccctcg cgcgcgctcg actgtgcgca
     2521 ggcgctcgcg gacggtggcg cgcaggccat cgtcgtccat gcgcgcaccc gcgagcaggg
     2581 ctaccgcgcg cccgcgcact gggagtgggt ggcgcgcatc ggcgacgcgg tgcgcgtgcc
     2641 ggtgaccgcc aacggcgagg tgtggagcgt gcgcgactgg gcacgctgcc gcgaggtctc
     2701 cgcagccgag gatgtgatga tcggtcgcgg cgcggtctcc gacccctggc tcgcgcgccg
     2761 catccgcggc gaacgcggca ccgagccgga cgagcgcgac tgggtcgaac tgcacccgca
     2821 cctcctgcgc tactgggagg gcgtgcaggg gcgcgccgcc ccggtgcatg cctgtggccg
     2881 gctcaagctg tggctgggca tgctgcggcg caactacccc gcggcgggcc ggctgcacga
     2941 ggcggtgcgc cgcgtcgtcc acccctcgcg catgaacgag gaactccgcc gccacggcat
     3001 tcccgcgtgc gaccacccgc catctgcccg ggccgacggg ccccggccga cgagtcccga
     3061 ccgatgagag cggcccgatg agctacgcca actcccgcat cccgcagagc gcccagcagg
     3121 gcgtccacga ggcgctcgcc gagcgcgtgc gcaagcacct cgagcatccc ttccgcaagc
     3181 cctgcaccga ctacaaccgt gccgcgctcg ccgaggcgct cgccggctgg ggccgcagtg
     3241 cgccgctgat cctcgatgcc ggctgcggcg tcggccacag caccatccag atcgcgcgcc
     3301 agtttcccga ccactgggtg atcggcgtcg accagtccgc cgaccgcctc gcgcgtcgca
     3361 agccctatcc cgaggcgctg ttgccgaaga acatggtctt cgtgcgcgcc gacctggtgg
     3421 acttctggcg cctgctcgac gaggccgggc tgcgcctcgc gcgccactac ctcctctatc
     3481 ccaacccctg gcccaagatc ggccacctcg gccggcgctg gcacgcgcac ccggtgttcc
     3541 cgtggattcc ccgcctcggt ggcgtgctgg aatgccgcag caactggaag gtctacatcg
     3601 aggagttcgc cttcgcgctc ggcctcgtgc tcgggcgcga ggtcgcctgg gaaggcttcg
     3661 aggccgtcac gccgctgacc cccttcgagc gcaagtaccg cgactcgggg cagccgttgt
     3721 accgcatcgg gctggatctc gcggccgcgg gctgattccg caagatcgtg cttgcccgcc
     3781 gcgccgcctt gggccaccct tggcgccggt caaaggagat tgccatgaac ggtatggaac
     3841 aaaggggcgc ggacgcgtcc gcgctgcgca cgatgagcga cgaggatttc gaggcgctgg
     3901 aagagatcct cacctcggac atcgtgcccg aggactgcat ggacctcgag atgctcgacg
     3961 gctacctcgc cggcgtgatg atctcgccgc ggccgatccc ggccgagcgc tggatgcccg
     4021 gcgtgtggtc ggcgcacggc gacgaggtcg gcttcggctc gggcagcggc ctgcagcgcg
     4081 cgatccggct ggtgaaggcg tatcacaacg agctgctcgc cacgctcggc ctggacgacg
     4141 aggacgaggc gtgctgggag ccgttctgct tcgcggtcgc cgagggcgat gggctcaagc
     4201 tcggcgaagc ctggatcgac ggtttcgctc agggcctgga cctgtggccc gagggctggg
     4261 aggaggggct ggacgccgag gcggtcgagg ccgtgcgcga gaccctcgac gaggtgctcg
     4321 cgccctgggg cgaggaggac gccgcgaagg ccgacgacga gacccgcctg ggttggctcg
     4381 ccgacgtggg cgaggcggtc aacgacatct tcgcccactg gcgcgatctg ggtctgccgg
     4441 ccgccgagcc gctgtccacc gaggctccgc gcaagcccgc cagcgccggc cccggccgca
     4501 acgatccgtg cccgtgcggc agcggcaaga aatacaagaa atgctgcggc gccaacggcg
     4561 ccgagcccga gcccgaatcc ggcgaagccg aaacctgagc atcccgggcg tgccggcgcg
     4621 cacaggaccg tcgcgcccgg ggacccgctg ccggagcggc actagtcgcg agcgggcgcc
     4681 ggaacagcgg acgggcgttg gcgagcgtgg acaagccgta gtcgcgactt gcgtcgctcc
     4741 cacaaggaac cgccgcgccc ggggacgcgc tgcgggcgcg atccgatgcg agcgggtttc
     4801 ccgctccgga aaatatttct caaaatgatt tcaggttact gaaaacaaag cgctttgcac
     4861 cactttccgg cgcctcccgg cggcgctaga atgcgcgccc cacggccctc ggccggtcct
     4921 ttcggcgctc cgttcatgga cgagttcaac ccctgcctcg actgcggcat ctgctgcacg
     4981 cactttcgga tctccttcta ctgggccgag gcggacgacg cgccgggcgg tttcgtgccg
     5041 gcggagatga ccgagaaact caaccacacc ttgcgctgca tgaagggcag caaccaggtg
     5101 ccgcgacgct gcagcgcgct ctccggcgag gtgggccggc aggtcgcgtg ttcgatctac
     5161 gagaaccggc ccaccccctg tcgtgaattt cccgtctatt tcgaggacgg tacgcccaat
     5221 ccgaagtgcg acgagctccg cgccaccata ggcctgccgc cgctgccgca gagtccgctg
     5281 ccgcgcgcgg cctgatcgcc ggatacgtct cgccgcctat ccgccgcgcg gccggctcac
     5341 cgcggcgagc acctcgagcg ggccatgtcg gcgcaagccg tcgatctggg cgcggtcgcg
     5401 cacgaacagc gagccggccc aggcgagcga attcagcgac acgtcgtccc atttctcgcg
     5461 tgtgcggggc accagcagca gcgcgccacg gctgaccagc aggttgtagg gcggcatcgg
     5521 atcggcgtcg cagggaagct ccagccgctc gcaggccagg cggtaggcgg ccagaaggac
     5581 cgatccggcg tcgtccgggt gcgaccaggc ggattcgtcc atccccaccg ccgcgtgcgc
     5641 ccagggcagg gcgaggttct ccgctgccgc cccgggacgc agcggaggca ggaagtcttc
     5701 cagcgccgag gcctgcgcgg gcacccactg caggtgcttg tgcgcctggc tcgcccccgc
     5761 gatgcggccg ccgttgtaga agcccagccc gccgtgcgcg cccactaccg cggcgagcgc
     5821 ggcgaagtcc gcgccgccga gcggcgtgct ctggtcttcg aaacggcggg tcacgatgag
     5881 caggtgacga tcgatcaccg ggaacttgtt cagcaccacg aggtgatccg cgccgagggc
     5941 cgccaccgtg agcgcaggct cgggcggcag gaaggggttg aagtcgggcc gccgggcagt
     6001 gaccgtgtcg acccgcgccc gatccttgag cgcgagggac gatacccaac gcaccgagaa
     6061 gcgcaggccg tcctgctcca gccagtcgag ctcggtacgg atcggctgca atgcgccgct
     6121 ggcgagcgcc gcggcgatca cgtggtcggt gcgctcgagg aggtcgtccg cgcgcgagcg
     6181 ggcgctcgac ggcggatggg cggtgagcgc tgcggcgtcg gacggagaac ggtgggcggg
     6241 cgccgaggcg ctggccgggg cggcgtcgct tgccggatcg cgggcggtgg gcgtgctggt
     6301 catgggcggg cgtgcgggag acaatgccgc cattttgaca cgaggcccgc gcgcgcagcc
     6361 gtggcgggca ggagggatcg cgatggcgat acggttcgaa gtgcgcggtg aatacattca
     6421 gctcgaccag ttgctcaagg ccgccggtct ggtcgagtcg ggcggtgcgg cgcatgccgc
     6481 ggtggaaaac ggcctggtga aggtcgacgg caagcccgag tcgcgcaagc gcgccaagct
     6541 gcggccggga cagcgggtgt cgttcgccgg cgaggcggtg gaactcgtgg aggaaggcgc
     6601 gggctagcag gtctcgcaga cgcggcgcac gtcgcgatcg gcgctcgagc ggcggggccg
     6661 tgcccggcaa cgtggggagc ggctcagggc agctgcggcg agcgtttccc gtgcttgcgc
     6721 tgcgcgagca tttcccagac ccgtccgccg ggcaggtcgc ggccggtgag gatgtattcg
     6781 agggtgtgct cgttggcgaa gatcagggcg acatcctcga gcgcgcggcg ggtgccggcg
     6841 aagagcaatt cgtcgccggg gcggagcttc tcgtcggcgg cgggcgtcat ccggtggtcg
     6901 tcgtcgtcgc gctcgaggta gagcaccgcg cagtcgagcg ccatcgagcg gtcggcgggc
     6961 gagcgcagca ggcgttcgag cgtgatcgtc tcgccgcgca tcaggcggcg atacagcgcc
     7021 ggcgcgctcg acaggttgac gcgctggctg cggatccgcg gaaccttcca gccgaggtgg
     7081 cgggtcaggc gatgcagcag tgcgccgcac cactcctcgt cacggcggcg gatttcggcc
     7141 aggaagggcg cgagcagcgg tgtgctcagg atcgcgatgc attcgtgcgc gatcacccgg
     7201 ctcgggacca cggtgatgtc ggattcgaag gcctcgaaga gcgcgcggtt ggattcgtgg
     7261 ttctggcgca ggat
//
LOCUS       DAOQNN010000143         7226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5489_length_7226_cov_10.539534, whole genome shotgun sequence.
ACCESSION   DAOQNN010000143 DAOQNN010000000
VERSION     DAOQNN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5489_length_7226_cov_10.539534"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PKV97_09055"
     CDS             complement(<1..604)
                     /locus_tag="PKV97_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNB06061.1"
                     /translation="MRWFFRFLLVFGALAGVLAVTLAAGLRQGLLALLGVGFGAVLQG
                     ARFGFTTGWRDMIERRDPQGLWAQMLLMVLAAALTLPLLAGSGGELVGAVAPLTISLV
                     LGAFLFGAAMQLADGCGSGTLYKAGGGSPLSLVVLPTFAFGSFLGASHQPAWIELGGL
                     PAVDLLAFGWPTALALTVAGCGLVAWLAGRAARRAGGDAPP"
     gene            complement(618..>1106)
                     /locus_tag="PKV97_09060"
     CDS             complement(618..>1106)
                     /locus_tag="PKV97_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB06062.1"
                     /translation="APQVAPSQFSYRYDQEQVVTSEELAAAVKAGNAPLLVDARPPKF
                     FAGETRTDAAARYGTLPGAKSFDNAQFFPQGGTTLKPASELKALVAQFGAAAAPAVSF
                     CNTGHWAATNWFVMSEIAGNKAVKLYPSSVVEWSQSELPMDNQPSRLSALVQDAKRAL
                     DK"
     assembly_gap    1107..1206
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1384..2406
                     /locus_tag="PKV97_09065"
     CDS             1384..2406
                     /locus_tag="PKV97_09065"
                     /inference="COORDINATES: protein motif:HMM:NF023984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HNB06063.1"
                     /translation="MVVSSVGSVCWGPARRTARRCHDAPQNTGKLKYSKRLSKKKFIT
                     FQNENDGCAGVELDARAAIEAQLALRPRNRKNRLNTLSIGLRILAVLLLLAVGLSFAN
                     DPRPAASGAEGGWRGAARHSAGIAPDPERFADTAIVQVYAAATYGWRGYFAVHPWIIY
                     KRAGETVYTRYDVIGWGRGNVVRRNYALPDGLWFGAVPRVLVEHRGEGVEAMIGQIEA
                     AVASYPHADEYRSYPGPNSNTFLAHIGREVPALRLDLPANAIGKDYRPVTRPLGVSPS
                     GSGVQFSLLGLLGLSVGVQEGIELNVLGLNFGLDLNRPGLRLPAIGRLGLDDASVSER
                     GAAGLQ"
     gene            complement(2488..3411)
                     /locus_tag="PKV97_09070"
     CDS             complement(2488..3411)
                     /locus_tag="PKV97_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB06064.1"
                     /translation="MSHTPQPARWLPFVVLGIGLAAISFGAIFARLAQAEGVSSLAVA
                     TWRLGFAALIITPVAFLQSRHELARLTRRQIGMALAAGFFLALHFATWISSLEYTSVA
                     SSTALVTTNPLWIGLASFLIFRETPTKMMIGGIALSFAGSLFIFWSDSQTAGAGSNPM
                     FGNLLALVGSWCFSAYLLIGRRLRAGLGLPAYIWLAYGAAALFLFAASGAGGVELFSL
                     SNTAWLVLLAMALGPQLLGHTAYNWSLRYVSATFVAVVTLGEPVGSALMAFLVFGEGF
                     APLQFVGFSLLLVGIYLAAKGERAEKGAGSD"
     gene            complement(3756..4469)
                     /locus_tag="PKV97_09075"
     CDS             complement(3756..4469)
                     /locus_tag="PKV97_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB06065.1"
                     /translation="MLKMEGVHAHYGHIHALRGIDVEVQVGEIVTLIGANGAGKSTLM
                     MSLFGNPKASAGRIVFDGEDITRVHTHEIARRGIALVPEGRRIFPRMTVIENLQMGTA
                     HADPANYAADTARVFAMFPILQERQAQRAGTLSGGEQQMLAIGRALMSRPKLLLLDEP
                     SLGLAPIYIRRIFQIVRELNRDHGMTILLVEQNANQALRVADRGYVLQHGEITLAGTG
                     AELLASPEVRAAYLEGGHA"
     gene            complement(4469..5302)
                     /locus_tag="PKV97_09080"
     CDS             complement(4469..5302)
                     /locus_tag="PKV97_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB06066.1"
                     /translation="MNTPATLLSVRNLTMRFGGLTAIDDLSLEVPAGKITGIIGPNGA
                     GKTTLFNCLTGFYKPTTGTLRLAHPTRGEMRLESLPSHRVARDAQVVRTFQNIRLFPK
                     MTVLENLIVAQHNVLQRASKFSIAGIFKLPGYRQAERQAIERAAMWLERLKLTHLADD
                     AAGDLPYGIQRRIEIARALCVDPVLLCLDEPAAGLNPRESAELNELLLYLCGEMGIAI
                     LLIEHDMSVVMNVSDHIGVISYGRKIAEGTPAQVKHDPQVIKAYLGEDEEEEVTGGAA
                     A"
     gene            complement(5299..6630)
                     /gene="livM"
                     /locus_tag="PKV97_09085"
     CDS             complement(5299..6630)
                     /gene="livM"
                     /locus_tag="PKV97_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HNB06067.1"
                     /translation="MSAVVFARHAMTPAERLRDAGWVAFVGLILGIPLVGLTTVDVGG
                     RLGVETRFGLLAAAVAAVFAGRLLLRWAIDQARNRKQERDRASTATAADRAHGAQRVV
                     NVLGWVALGASIALPIVFSDNRYVVDTATTVLIYVMLGWGLNVVVGLAGLLDLGYVAF
                     YAVGAYSYALLSTQFGWSFWEALPVSGALAASFGILLGYPILRLRGDYLAIVTLGFGE
                     IIRIILVNWTEFSNGPNGISSIPRPSFFGLDFTRSAPEGGQTFAGFFGLEFSPMHRLI
                     FLYYLILVLALLTHAFVSRLRKLPVGRAWEALREDEIACQAMGMNTTNIKLSAFAIGA
                     MLGGFAGVFFAARQGFISPESFTFTESAIILAIVVLGGMGSQMGVVLAATLLVLIPEF
                     GRNFAEYRMLLFGLAMVLIMVWRPGGLLSHREPTVHLNASGKTAATKEAAR"
     gene            complement(6630..>7226)
                     /locus_tag="PKV97_09090"
     CDS             complement(6630..>7226)
                     /locus_tag="PKV97_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931192.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HNB06068.1"
                     /translation="AIGMSIFLQNSVQLTQGARVKPIPPVLEGGIVLWEAPGFTVQIG
                     WSQMLIIVTTVILMSAFTWMITQTPFGRQQRACEQDRTMAALLGVNVDRTISYTFMLG
                     AALAAVAGVMVTVYYGVVDFFIGFLAGIKAFTAAVLGGIGSLPGAMLGGLLIGLIESF
                     WAAYLWAEYKDVATFGILCLVLMLRPSGLLGRPEVEKV"
BASE COUNT         1132 a         2348 c         2609 g         1037 t
ORIGIN      
        1 agggcggtgc gtcgccaccc gccctgcgtg cggcgcgccc ggccagccag gccaccaggc
       61 cgcagccggc cacggtgagc gcgagcgcgg tcggccagcc gaaggcgagc aggtccaccg
      121 cgggcaggcc gccgagctcg atccaggcgg gctggtgcga ggcgccgagg aagctgccga
      181 aggcgaaggt gggcagcacc acgagggaca gcggcgagcc gccgccggcc ttgtagagcg
      241 tgcccgagcc gcagccgtcg gcgagctgca tcgccgcacc gaacaggaag gcgccgagca
      301 ccaggctgat cgtcagcggc gccactgcgc cgaccagctc gccgccgctg ccggcgagca
      361 gcggcagggt gagcgccgct gccagcacca tcagcagcat ctgcgcccac aggccctgcg
      421 ggtcgcggcg ctcgatcatg tcgcgccagc cggtggtgaa gccgaaacgt gcgccctgca
      481 gtaccgcgcc gaagccaacg cccagcagcg cgagcaggcc ctggcgcagg ccagccgcca
      541 gcgtcaccgc gagcacgccc gcgagtgcgc cgaagaccag caggaatcgg aagaaccagc
      601 gcatcgtcgc gtccgcctta cttgtcgagc gcgcgcttgg cgtcctgcac cagcgcgctc
      661 aggcgcgagg gctggttgtc catcggcagc tcggactggc tccattccac caccgaggag
      721 gggtagagct tgaccgcctt gttgccggcg atctccgaca tcacgaacca gttggtggcg
      781 gcccagtggc cggtgttgca gaaggacacc gcgggtgcgg cggcggcgcc gaactgcgcg
      841 accagggcct tgagctcgct cgccggcttg agcgtggtgc cgccctgggg gaagaactgc
      901 gcgttgtcga acgacttcgc gcccggcagc gtaccgtagc gcgcggcggc gtcggtgcgc
      961 gtctcgccgg cgaagaactt gggcgggcga gcgtcgacca gcagcggtgc gttgccggcc
     1021 ttgaccgcgg cggcgagctc ctcgctggtg acgacctgct cctggtcgta gcggtagctg
     1081 aactggctgg gcgcgacctg cggcgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnccgg gctggccgag gagggtcttc agttcggccg gctggagcag cggggcgggg
     1261 gcggcggtcg tcgccaccgc ggcggcgggg ctttgtccag tggtgctggc gaaggcgggc
     1321 gtggcgaagc cgaggctggc ggcgagcgcg atggtcaggg gcaggggggc gagtttcttc
     1381 agcatggtcg tgtcgtccgt cggttccgtt tgctgggggc cggcgcgccg gactgcgcgg
     1441 cgctgccatg atgcgccgca gaatacggga aagctgaaat actcgaaacg tctttcaaag
     1501 aaaaagttta taacttttca gaatgaaaac gacggctgcg ccggcgtaga actcgatgcg
     1561 cgagcggcga tcgaagcaca actggccttg cggccccgca acaggaaaaa ccgcttgaac
     1621 accctctcga tcggcttgcg catccttgcc gtcctgctgc tgcttgcggt ggggctcagc
     1681 ttcgccaacg atccgcgtcc cgcggcgtct ggcgcggagg gcggctggcg cggcgcggcg
     1741 cgccactccg ccggcatcgc acccgacccc gagcgcttcg cggataccgc gatcgtgcag
     1801 gtgtatgccg cggccaccta cggctggcgc ggctacttcg cggtgcatcc gtggatcatc
     1861 tacaagcgtg ccggcgagac cgtctacacc cgctacgacg tcatcggctg gggacgtgga
     1921 aacgtggtgc ggcgcaacta cgccttgccc gacgggcttt ggttcggggc cgtgccgagg
     1981 gtgctggtcg agcaccgcgg cgaaggcgtg gaggcgatga tcgggcagat cgaggccgcg
     2041 gtggcgagct acccgcacgc ggacgagtac cgcagctatc cgggcccgaa cagcaacacc
     2101 ttcctcgccc acatcggccg cgaggtgccg gcgctgcgcc tcgacctgcc ggcgaacgcg
     2161 atcggcaagg actatcgccc cgtcacgcgt ccgctgggcg tgtcgccctc ggggtcgggc
     2221 gtgcagttct cgctgctcgg cttgctgggc ttgagcgtgg gcgtgcagga aggcatcgag
     2281 ctgaacgtcc tcgggctcaa tttcggcctc gatctcaacc gtcccggcct gcgtctgccc
     2341 gcgatcggcc gcctgggcct cgacgacgcg tcagtgtccg agcgcggcgc cgccgggctg
     2401 cagtgacgcc gagaggccgg cgcgcgggct tcggcagcgc cgcgctcctc gccgccgctt
     2461 cgggatcgat gcgttgcggt cgcctcgtca atcgcttccc gcgcccttct cggccctctc
     2521 gccctttgcc gccaggtaga tgcccaccag cagcaggctg aagcccacga actgcagcgg
     2581 cgcgaagcct tctccgaaga cgaggaaggc catcagcgcg ctgcccaccg gctcgcccag
     2641 cgtgaccacc gcgacgaagg tggcagacac gtagcgcagc gaccagttgt aggcggtgtg
     2701 gccgagcagt tgcggtccga gcgccatggc caacagcacc agccaggcgg tgttcgacag
     2761 gctgaacagt tccacgccgc ccgcgccgct ggcggcgaac aggaacagcg ccgcggcgcc
     2821 gtaggcgagc cagatgtagg ccggcagccc gaggccggcg cgcaggcggc ggccgatcag
     2881 caggtaggcg gagaagcacc agctgccgac gagcgcgagg aggttgccga acatggggtt
     2941 gctgccggcg ccggcggtct ggctgtcgct ccagaagatg aacaggctgc cggcgaagga
     3001 cagcgcgatg ccgccgatca tcatcttcgt cggcgtctcg cggaagatga gaaaggaggc
     3061 caggccgatc cacagcgggt tggtggtgac gagcgcggtg ctcgaggcga ccgaggtgta
     3121 ttcgagcgag ctgatccagg tggcgaagtg gagcgcgagg aagaagcccg cggccagcgc
     3181 catgccgatc tgcctgcggg tgaggcgggc gagctcatgg cgcgattgca ggaaggcgac
     3241 cggcgtgatg atgagcgcgg cgaagcccag ccgccaggtc gcgaccgcga gcgagctcac
     3301 cccctcggcc tgggcgaggc gggcgaagat ggcgccgaag gagatcgcgg cgaggccgat
     3361 gccgaggacg acgaagggga gccagcgggc gggttggggg gtgtgggaca tctggagagg
     3421 ggcgattggt gggtgaggtg ggtcgagagc accctgaggg gcttcgaccg gtggcgatgc
     3481 gtgttcgaac cgttcgcggg gacggcacat gtgtgccggc actcgggtgc ttccggaggc
     3541 gtggcggttt tccgtaggag cgacgcaagt cgcgatcggg cagtgccgca gcgccgtcgc
     3601 gatttccgcc cgctgcaatc gcgacctgcg tcgctcctac ccgacgcatt gcggcgcgat
     3661 tgtgccgcat cgcgtcgaaa ccgcggcgtt tcgtctgatc gcggagcggc cccggttggc
     3721 gccattcctc tcgcgtcgca cgcctcacgc gcctctcagg cgtggccgcc ctccagatac
     3781 gccgcccgca cctccggact cgccagcagt tccgccccgg tcccggcgag ggtgatctcg
     3841 ccgtgctgca gcacatagcc gcggtcggcc acgcgcaggg cctggttggc gttctgctcg
     3901 accagcagga tggtcatgcc gtggtcgcgg ttgagctccc tgacgatctg gaagatccgc
     3961 cggatgtaga tcggtgccag gccgagcgag ggctcgtcga gcagcagcag cttggggcgg
     4021 ctcatcagcg cgcggccgat cgccagcatc tgctgctcgc cgcccgacag ggtgccggcg
     4081 cgctgcgcct ggcgttcctg caggatcggg aacatcgcga acacgcgcgc ggtgtcggcg
     4141 gcgtagttgg ccgggtcggc gtgcgcggtg cccatctgca ggttctcgat caccgtcatg
     4201 cgcgggaaga tgcgccggcc ttcgggcacc agggcgatgc cgcggcgggc gatctcgtgc
     4261 gtgtgcacgc gggtgatgtc ctcgccgtcg aagacgatgc gcccggcgct ggccttgggg
     4321 ttgccgaaca ggctcatcat cagcgtcgat ttgccggcgc cgttggcgcc gatcagggtg
     4381 acgatctcgc ccacctggac ctcgacgtcg ataccgcgca gcgcgtggat gtggccgtaa
     4441 tgggcgtgca cgccctccat cttgagcatc atgccgcggc ccctccagtc acttcttctt
     4501 cctcgtcctc gcccaggtag gccttgatca cctgcgggtc gtgcttgacc tgcgccggcg
     4561 tgccctcggc gatcttgcgc ccgtagctga tcacgccgat gtggtcggac acgttcatca
     4621 ccacgctcat gtcgtgctcg atgagcagga tcgcgatgcc catctcgccg cacaggtaga
     4681 gcaggagttc gttcagctcc gccgactcgc gcgggttgag gccggcggcc ggttcgtcca
     4741 ggcacagcag caccggatcg acgcacagcg cgcgtgcgat ctcgatgcgg cgctggatgc
     4801 cgtagggcag gtcgccggcg gcgtcgtcgg cgaggtgggt gagcttgagg cgctcgagcc
     4861 acatcgccgc gcgttcgatc gcctgccgct ccgcctggcg gtagccgggg agcttgaaga
     4921 taccggcgat ggagaacttc gaggcgcgct gcagcacgtt gtgctgggcg acgatcaggt
     4981 tctccagcac cgtcatcttg gggaacaggc ggatgttctg gaaggtgcgc accacctggg
     5041 cgtcgcgtgc gacgcggtgg ctgggcaggg actccaggcg catctcgccg cgcgtggggt
     5101 gggccaggcg cagcgtgccg gtggtgggct tgtagaagcc ggtgaggcag ttgaacagcg
     5161 tcgtcttgcc ggcgccgttg gggccgatga tgccggtgat cttgccggcc ggcacttcca
     5221 gcgagaggtc gtcgatggcg gtcaggccgc cgaagcgcat ggtcaggttg cgcaccgaca
     5281 gcagggttgc gggcgtgttc atcgcgcagc ctccttcgtc gcggcagtct tgcccgacgc
     5341 gttcaggtgc accgtcggtt cgcggtgcga cagcaggccg cccgggcgcc acaccatgat
     5401 cagtaccatc gccaggccga acagcaacat gcggtactcg gcgaagttgc gtccgaactc
     5461 ggggatcagc accagcagcg tggccgccag caccacgccc atctgcgagc ccatgccacc
     5521 gagcacgacg atggcgagga tgatcgccga ctcggtgaag gtgaagctct cgggcgagat
     5581 gaagccctgg cgcgcggcga agaacacccc ggcgaagccg cccagcatgg cgccgatggc
     5641 gaaggccgag agcttgatgt tggtggtgtt catgcccatc gcctggcagg cgatctcgtc
     5701 ctcgcgcagc gcctcccagg cgcgccccac cggcagcttg cgcaggcgcg acacgaaggc
     5761 gtgggtgagc agggccagca ccaggatcag gtagtagagg aagatcaggc ggtgcatcgg
     5821 cgagaattcg aggccgaaga agccggcgaa ggtctgcccg ccttccggcg cgctgcgcgt
     5881 gaaatcgagg ccgaagaagc tcgggcgcgg gatcgagctg atgccgttgg ggccgttgga
     5941 gaattcggtc caattcacca ggatgatgcg gatgatctcg ccgaagccca gcgtgacgat
     6001 cgccaggtag tcgccgcgca ggcgcaggat cgggtagccg agcaggatcc cgaagctggc
     6061 ggcgagcgcg cccgataccg gcagcgcctc ccagaagctc cagccgaact gggtcgacag
     6121 cagcgcgtag gaataggcgc ccaccgcgta gaaggcgacg tagccgaggt cgagcaggcc
     6181 ggcgaggccg acgaccacgt tgaggcccca acccagcatc acgtagatca gcaccgtggt
     6241 ggcggtgtcg accacgtagc ggttgtcgga gaacacgatc ggcagcgcga tcgaggcgcc
     6301 gagcgccacc cagccgagca cgttcaccac gcgctgcgcg ccatgggcgc ggtcggcggc
     6361 ggtggcggtg ctggcgcggt cgcgttcctg cttgcggttg cgcgcctggt cgatcgccca
     6421 gcgcagcagc aggcggccgg cgaacaccgc ggccacggct gcggcgagca ggccgaagcg
     6481 ggtctccacg cccagccggc cgcccacgtc caccgtggtg aggccgacca gcgggatgcc
     6541 caggatgagg ccgacgaagg ccacccagcc ggcgtcgcgc agacgctcgg ccggcgtcat
     6601 cgcgtgacgc gcaaagacga cggcgctcat cagaccttct ccacttcagg ccggccgagc
     6661 aggccggagg ggcgcaacat cagcaccagg cacaggatgc cgaaggtggc gacgtccttg
     6721 tattccgccc acaggtaggc ggcccagaag gattcgatca agccgatcag caggcccccg
     6781 agcattgccc ccggcagcga gccgatgccg cccagcaccg cggcggtgaa ggccttgatg
     6841 ccggcgagga agccgatgaa gaagtccacc acgccgtaat acaccgtgac catcaccccg
     6901 gcgaccgcgg cgagcgccgc gccgagcatg aaggtgtagg agatggtgcg gtcgacgttg
     6961 accccgagca gcgcggccat ggtgcggtcc tgttcgcagg cgcgctgctg gcgaccgaag
     7021 ggggtctgcg tgatcatcca ggtgaaggcg ctcatcagga tcaccgtggt gacgatgatc
     7081 agcatctggc tccagccgat ctgaaccgtg aagcccggcg cctcccacag cacgatgccg
     7141 ccttcgagca ccggcgggat cggcttcacg cgtgcgccct gggtgagctg cacgctgttc
     7201 tgcaggaaga tcgacatgcc gatcgc
//
LOCUS       DAOQNN010000144         7156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5588_length_7156_cov_8.796085, whole genome shotgun sequence.
ACCESSION   DAOQNN010000144 DAOQNN010000000
VERSION     DAOQNN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5588_length_7156_cov_8.796085"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PKV97_09095"
     CDS             complement(<1..215)
                     /locus_tag="PKV97_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB06069.1"
                     /translation="MPAVRAALPWLAVLLLALAGLGTDYALGKATLVATYAIAGLGVV
                     VVVGQAGQIALGQAALVALGAYAQALLV"
     gene            complement(239..1105)
                     /locus_tag="PKV97_09100"
     CDS             complement(239..1105)
                     /locus_tag="PKV97_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004386118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB06070.1"
                     /translation="MLAFVQVLASGVAIGCVYGLVALSFVLIYKATETVSFMQGDLLM
                     LGAFAALGLHAFAGWPLVAAAVAAVLAVGVLGAVLERAVLRRALGQPHLVAVLLTFGL
                     GMMMRGGVASVPAAAQDMHRLPFAADTLAIGPLVLAASHLWVIAATVLLAGLLAAFFR
                     HTRTGLALRACSEDARSAALMGIPVARMHTLAWALGAALAAVAGLLLAPITFVHLNMG
                     FIALKAFPAAVLGGLTSLPGALVAGIFLGVAEALAGLALPEGTKDFVPYVLLLAALLL
                     FPQGLGGARGRA"
     gene            complement(1398..3563)
                     /locus_tag="PKV97_09105"
     CDS             complement(1398..3563)
                     /locus_tag="PKV97_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06071.1"
                     /translation="MSDETSKKGYQWRFFRSGGFDQVRIETADDLRHLGELDQKLWSV
                     LACPTSGLEFDSRTLQLLDVDNDGSIRAPEIIDATRWVCTVLKDPGVLFQGGDGLPLA
                     AIDETNAEGAKLLATASKVLAYVGKADAGEISMGDLAETEKLFAPEHQNGDGVVPAEL
                     AGDPRLAGAITRIVETYGAAEDRSGKPGVDQARVDAFFAAAQEVSDWHARAEADAATV
                     LPLGDATGAAAAVFEAVREKVEDYFTRCRLAAFDERAAAALNPADTAYVELSLQSLDA
                     ASAAVAALPLALVGPGRALPLAVGLNPAWEARIAALRDEVVTPILGAREVLTQAEWQD
                     LAGRFAAHRAWVAAMPANPVAVLPVADVRALLADGTCAALTRLIDEDLAAETAAGQVD
                     ALERLVRYNRDLVRLLRNFVTLSDFYSRHDKAIFQAGTLYLDQRSCELCLRVGDMARH
                     ATLAPLSGTYLVYCECQRQGEAPMNIVAAMTGGDADEMMVPGRNGIFYDRQGRDWRAS
                     VVKVVEAPISVRQAFWSPYKRIGRMIGEQLQKFAAARDKAVNDQAATGVAGASAKIEA
                     APAAPAAPAAPFDIAKFAGIFAAMGLAVGAIGTALAAVVSAFLGLPVWQMPLVVVGAV
                     LVISGPSMLLAWLKLRQRNLGPLLDANGWAVNTRARINIPFGGALTGVAALPPGASRA
                     LTDPYAEKKTPWKSWLFLVLLIAAVVVAWQQGYLDRFLG"
     gene            complement(3754..4395)
                     /locus_tag="PKV97_09110"
     CDS             complement(3754..4395)
                     /locus_tag="PKV97_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB06072.1"
                     /translation="MIQATIVPVTPFQQNCSILWCERTKKAAVVDPGGDLDRILAAVA
                     ELGVTVEKILITHGHIDHAGGTAKLARELGVPIEGPQEEDRFWIEGMPQQSKMFGFPD
                     VESFEPDRWLHDGDTVTVGEQTLKVIHTPGHTPGHVVFVHEGARLAIVGDVLFAGSIG
                     RTDFPRGDHQTLIRSIREKLFPLGDDITFIPGHGPTSTFGEERDSNPFVGAYG"
     gene            complement(4673..6016)
                     /gene="gdhA"
                     /locus_tag="PKV97_09115"
     CDS             complement(4673..6016)
                     /gene="gdhA"
                     /locus_tag="PKV97_09115"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNB06073.1"
                     /translation="MKYQSLEEFLAYVEQRNPGQPEFLQAVTEVMESLWPFISANKRY
                     AEHSLLDRLIEPERAISFRVSWVDDRGEVQVNRGYRIQHSSAIGPYKGGLRFHPSVNL
                     SILKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGRSPGEVMRFCQAFMTELYRHVGPD
                     TDVPAGDIGVGGREVGFMAGMMKKLSNNSACVFTGKGLSFGGSLIRPESTGYGTVYFA
                     NAMLERAGRDIKGMRVSVSGSGNVAQYAIEKLMELGAKPITCSDSSGTVIDEAGFTPE
                     KLAILMEIKNHYYGRASEYAERVGARFEPGVRPWHVPVEVALPCATQNELDGNDAATL
                     VKNGVIAVAEGANMPSTMEAAKIFEHNGVLYAPGKASNAGGVATSGLEMSQNAMRLSW
                     TREEVDARLKEIMLGIHAACLKYGEREDGTVSYVDGANVAGFVKVAEAMLAQGVI"
     gene            complement(6013..6192)
                     /locus_tag="PKV97_09120"
     CDS             complement(6013..6192)
                     /locus_tag="PKV97_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06074.1"
                     /translation="MNASNVIVGAGLTKIMHQRGELMHNIGLHCAMASGKTEAIACAR
                     AQEKPVFNQSMGPTS"
     gene            6321..6797
                     /gene="lysM"
                     /locus_tag="PKV97_09125"
     CDS             6321..6797
                     /gene="lysM"
                     /locus_tag="PKV97_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HNB06075.1"
                     /translation="MGLFAFIKEAGEKLFGTGEANAAATDPAAANARAAQAIHNYIVA
                     LKLAPADLGVSFDAAKSLVVVSGTAPDQATREKILLAAGNVAGVAEVENRLSVARAEP
                     EAQFHTVARGDTLSAIAKKYYGNANQYPAIFEANKPMLSHPDKIYPGQVLRIPPQA"
     gene            6894..>7156
                     /locus_tag="PKV97_09130"
     CDS             6894..>7156
                     /locus_tag="PKV97_09130"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB06076.1"
                     /translation="MARIRIELPERFTFSTDIPLLVSHINYGNHLDNAQLLGIVSEAR
                     VRFLKSMGYSELDIEGLGIIVADAALQYRSEAFHGETMRVEMGA"
BASE COUNT         1120 a         2453 c         2552 g         1031 t
ORIGIN      
        1 accagcagcg cctgggcata ggcgccgagc gccacaagtg cggcctggcc gagggcgatc
       61 tggcccgcct ggcccaccac cacgaccacg ccgaggccgg cgatcgcgta ggtcgccacc
      121 agagtcgcct tgccgagcgc gtagtcggta cccagcccgg cgagcgcgag cagcagcacc
      181 gccagccagg gcaatgcggc gcgcaccgcc ggcacgccac cgcgcaggat gggtgcgctc
      241 acgcccgccc tcgcgcgccg ccgagcccct gcgggaacag cagcagcgcc gccagcagca
      301 gcacgtaggg cacgaaatcc ttcgtgcctt cgggcaacgc cagcccggcg agcgcctcgg
      361 cgacgccgag gaagatgccg gccacgagcg cgcccggcag gctggtcagc ccgcccagca
      421 ccgcggcggg gaaggccttc agggcgatga agcccatgtt cagatgcacg aaggtgatcg
      481 gcgcgagcag cagcccggcg accgcggcga gcgccgcgcc gagcgcccac gccagggtgt
      541 gcatgcgcgc caccgggatg cccatcagcg cggcgctgcg cgcgtcctcc gagcaggcgc
      601 gcagcgccag cccggtgcgg gtgtggcgga agaaggcggc gagcaggccg gcgagcagca
      661 cggtggcggc gatcacccac aggtggctgg cggcgagcac gagggggccg atcgccagcg
      721 tgtcggcggc gaaaggcagg cggtgcatgt cctgcgccgc cgccggcacc gaggcgacgc
      781 cgccgcgcat catcatgccc aggccgaagg tgagcagcac cgccaccagg tgcggctggc
      841 cgagcgcgcg ccgcagcacg gcgcgctcca gcacggcgcc gagcacgccc accgccagca
      901 ccgccgccac cgccgcggcg acgagcggcc agccggcgaa ggcgtgcagg ccgagcgcgg
      961 cgaaggcgcc gagcatcagc aggtcgccct gcatgaagct gaccgtctcg gtggccttgt
     1021 agatgagcac gaaggagagt gcgaccaggc catagacaca gccgatcgcg actccgctgg
     1081 cgaggacctg gacgaatgcg agcatggggg cgaagcatag ccgaagcgcc gcgcccgttc
     1141 gcgccccacg ggccgcggcg ccacctccac ccccgcgatg cttgatgcag gagcgccgca
     1201 aggcgcgaat gcggcggtgg aggcacgccc ccgcatcggc tccctgaccg ccgtcttcgc
     1261 gccttgcggc gctcccacgg ggaaagcggc aggcgtgtgc gccacgaaaa agggccggcc
     1321 ggcggtgcgg gggcatttac cccacacccg ccggccggcc ccgcatcggc ttgcttgccc
     1381 agccgggatg ctcccgatca gccgaggaat cggtccagat agccctgctg ccaggccacg
     1441 accaccgccg cgatcagcag cacgaggaag agccagctct tccacggcgt cttcttctcg
     1501 gcatacggat cggtgagcgc gcgcgaggcg cccggcggca gcgccgcgac gccggtgagc
     1561 gcgccgccga aggggatgtt gatgcgcgca cgcgtgttga ccgcccagcc gttggcgtcg
     1621 agcagcgggc cgaggttgcg ctggcgcagc ttgagccagg ccagcagcat cgacggaccg
     1681 gagatcacca gcaccgcccc cacgaccacc agcggcatct gccacaccgg caggccgagg
     1741 aaggccgaca ccaccgcggc gagtgcggtg ccgatcgcgc cgacggcgag acccatggcg
     1801 gcgaagatac cggcgaactt ggcgatgtcg aacggtgccg ccggtgcggc gggcgccgcc
     1861 ggggcggcct cgatcttggc gctggcgccg gcgacgccgg tcgcggcctg gtcattgacc
     1921 gccttgtcgc gcgcggcggc gaacttctgc agctgctcgc cgatcatgcg gccgatgcgc
     1981 ttgtacggcg accagaaggc ctggcgcacg ctgatcggcg cctccaccac cttgaccacg
     2041 ctggcgcgcc agtcgcgccc ctggcggtcg tagaagatgc cgttgcggcc gggcaccatc
     2101 atctcgtcgg cgtcgccgcc ggtcatcgcg gcgacgatgt tcatcggcgc ctcgccctgg
     2161 cgctggcact cgcaatacac caggtaggtg cccgacagcg gcgcgagcgt ggcgtggcgg
     2221 gccatgtcgc ccacgcgcag gcagagctcg cagctgcgct ggtcgagata gagcgtgccg
     2281 gcctggaaga tcgccttgtc gtggcgcgag tagaagtcgc tcagggtgac gaagttgcgc
     2341 agcaggcgga cgaggtcacg gttgtagcgc accaggcgct cgagcgcgtc gacctggccg
     2401 gccgcggtct cggcggcgag gtcctcgtcg atcaggcggg tgagcgcggc gcaggtgccg
     2461 tcggcgagca gcgcgcgcac gtcggcgacc ggaagcacgg ccaccgggtt ggccggcatc
     2521 gccgccaccc aggcgcgatg ggcggcgaag cgcccggcca ggtcctgcca ttcggcctgg
     2581 gtgaggactt cgcgcgcgcc caggatgggg gtgacgacct cgtcgcgcag cgcggcgatg
     2641 cgcgcctccc aggccgggtt cagcccgacc gccagcggca gcgcgcggcc cgggcccaca
     2701 agcgcgagcg gcagtgcggc gaccgcggcc gaggcggcat cgagcgactg cagcgagagc
     2761 tcgacatagg cggtgtcggc cgggttgagc gcggcggctg cgcgctcgtc gaaggcggcg
     2821 aggcggcagc gggtgaagta gtcctcgacc ttctcgcgca cggcctcgaa gaccgcggca
     2881 gcagcgccgg tggcgtcgcc gagcggcagc accgtggcag cgtcggcctc tgcccgggcg
     2941 tgccagtcgg agacctcctg cgccgcggcg aagaaggcgt ccacgcgcgc ctggtcgacg
     3001 cccggcttgc cgctgcggtc ctcggcggcg ccgtaggtct cgacgatgcg ggtgatcgcc
     3061 ccggccaggc gcggatcgcc ggcgagctcg gcgggcacca cgccgtcacc gttctggtgc
     3121 tcgggcgcga acagcttctc ggtctcggcg aggtcgccca tggaaatctc gcccgcgtcg
     3181 gccttgccga cgtaggccag caccttggag gcggtggcga gcagcttggc gccctcggcg
     3241 ttggtctcgt cgatggccgc cagcggcagg ccgtcgccgc cctggaagag cacgcccgga
     3301 tccttcagca ccgtgcatac ccagcgcgtg gcgtcgatga tctcgggcgc gcggatgctg
     3361 ccgtcgttgt cgacgtcgag cagctgcagc gtgcgcgaat cgaactccag gccggacgtc
     3421 ggacaggcca gcaccgacca cagcttctgg tcgagctcgc cgaggtggcg caggtcgtcg
     3481 gcagtctcga tgcggacctg gtcgaagcca cccgagcgga agaatcgcca ctggtagcct
     3541 ttcttgctgg tttcgtcgct catgttctcg aacactccct gtcgttatgc gggcagcacc
     3601 cgcaagccat gctcagggaa agatggtgcg cccgaaaatc gggtggaaca agcgatggac
     3661 ttcccgaatc cggctctctg cggccggact tgggcaactc ggacggagcg ccgaagaacg
     3721 ccagggacat ccactcccgg cgtgcgccga tgctcagccg taggcgccga cgaaggggtt
     3781 gctgtcgcgc tcctcgccga aggtcgaggt gggaccgtgg ccggggatga aggtgatgtc
     3841 gtcgccgagc gggaagagct tctcgcggat cgagcggatc agggtctggt ggtcgccacg
     3901 cgggaaatcg gtgcgcccga tcgagccggc gaagagcacg tcgccgacga tcgccaggcg
     3961 cgcgccctcg tgcacgaaga cgacgtggcc gggggtgtgg ccgggggtgt ggatgacctt
     4021 cagcgtctgc tcgcccacgg tgacggtgtc gccgtcgtgc agccagcggt cgggctcgaa
     4081 ggactcgacg tcggggaagc cgaacatctt gctctgctgc ggcatgccct cgatccagaa
     4141 gcggtcctcc tcctgcggcc cctcgatggg cacgccgagt tcgcgcgcca gcttcgcggt
     4201 gccgcccgcg tggtcgatgt ggccgtgggt gatgaggatc ttctcgaccg tgacgccgag
     4261 ctcggcgacc gcggcgagga tgcggtcgag gtcgccgccc gggtcgacca ccgccgcctt
     4321 cttcgtgcgc tcgcaccaca ggatgctgca gttctgctgg aacggggtga ccgggacgat
     4381 ggtggcctgg atcatgggcg acggactcct gttgacgggt ttgcgcggca gtttagcgga
     4441 tgcacgccgt cgacccgcac gagatgccgg cagcgggcgg ggcaggcgca tggggattgc
     4501 cgtgcaggag cgtcgagagg ctgcggttgc gtggaccgcc tgccgtcgat tcggaaacgt
     4561 cggattcgcg ccttgcggcg ctcctgcagg ggggctgcgg cggtttccgt aggagcgccg
     4621 caaggcgcga accccacgtg ccatgccggc gcgccggcac ggcacgcagg gatcagatca
     4681 cgccttgcgc cagcatggcc tcggcgacct tgacgaagcc ggcaacgttg gcgccgtcga
     4741 cgtagctcac cgtgccgtcc tcgcgctcgc cgtacttcag gcaggcggcg tggatgccga
     4801 gcatgatctc cttgaggcgg gcgtcgacct cctcgcgggt ccacgacagg cgcatcgcgt
     4861 tctggctcat ctccaggccc gaggtcgcca caccgccggc gttggaggcc ttgcccggtg
     4921 catacagcac gccgttgtgc tcgaagatct tcgcggcttc catggtgctg ggcatgttgg
     4981 cgccctcggc gaccgcgatc acgccgttct tgaccagggt ggcggcgtcg ttgccgtcga
     5041 gctcgttctg ggtcgcgcac ggcagcgcga cttccaccgg cacatgccac ggacgcacac
     5101 ccggctcgaa gcgggcgccg acgcgttcgg cgtattcgga ggcgcggccg tagtagtggt
     5161 tcttgatctc catgaggatc gccagcttct cgggggtgaa gccggcctcg tcgatcacgg
     5221 tgccgctcga gtcggagcag gtgatcggct tggcgccgag ctccatcagc ttctcgatgg
     5281 cgtactgggc gacgttgccc gagcccgaca ccgagacgcg catgcccttg atgtcgcggc
     5341 cggcgcgctc gagcatggcg ttggcgaagt agaccgtgcc gtagccggtc gactcgggac
     5401 ggatcagcga gccgccgaag gacaggccct tgccggtgaa cacgcaggcc gagttgttcg
     5461 acagcttctt catcatgccg gccatgaagc cgacctcgcg gccgcccacg ccgatgtcgc
     5521 cggcgggcac gtcggtgtcg ggaccgacgt gacggtacag ctcggtcatg aaggcctggc
     5581 agaagcgcat gacctcaccc gggctgcggc ccttgggatc gaagtcggag ccgcccttgc
     5641 caccgcccat cggcagcgtg gtgagcgcgt tcttgaaggt ctgctcgaag gcgaggaact
     5701 tcaggatcga caggttcacc gaggggtgga agcgcaggcc gcccttgtag gggccgatcg
     5761 cggagctgtg ctggatgcgg tagccgcggt tgacctgcac ctcgccacgg tcgtccaccc
     5821 aagagacgcg gaacgagatc gcgcgctcgg gctcgatcag ccggtcgagg agggagtgct
     5881 ccgcatagcg cttgttggcc gagatgaagg gccacaggct ctccatgacc tcggtcacgg
     5941 cctgcagaaa ctcgggctgg ccggggttgc gctgttcgac gtaggccagg aactcctcga
     6001 gggactgata tttcatgatg ttggacccat ggattgattg aagacaggtt tttcttgcgc
     6061 acgcgcacac gcgatggcct cggttttacc cgaggccatc gcgcagtgca ggccaatatt
     6121 gtgcatcaac tcccctcttt ggtgcatgat cttcgtcaaa cctgcgccga ctatgacatt
     6181 tgatgcattc atgcaccgtt cttgtgcgta acgatgaatg tcatccgacc ctgcgttcag
     6241 gcgccgtgca tggaccggga atgacccagg ggttatcctg accgtgcacg tgtagccaaa
     6301 ccaacgacag ggaggtgttc atggggctgt tcgcattcat caaggaagcc ggcgagaagc
     6361 tgttcgggac gggcgaggcc aacgcggcgg ccaccgatcc ggccgcggcc aatgccaggg
     6421 cggcgcaggc gatccataac tacatcgtcg cgctcaagct cgcgccggcg gacctgggcg
     6481 tgagcttcga cgccgcgaag agcctggtcg tcgtcagtgg caccgcgccc gaccaggcca
     6541 cgcgcgagaa gatcctgctt gccgccggca acgtcgctgg cgtggccgag gtcgagaacc
     6601 gcctgtcggt ggcgcgtgcc gaacccgagg cgcagttcca caccgtggcg cgcggcgaca
     6661 cgctgtcggc gatcgccaag aagtactacg gcaacgccaa ccagtatccg gcgatcttcg
     6721 aggccaacaa gccgatgctg agccacccgg acaagatcta tcctggccag gtgctgcgca
     6781 tcccgccgca ggcctgatca cgcatcaccg gcaggcggct ttgcgcttgc cccgccgagg
     6841 cccgggccgt tacgctcggg cctcttccgt ttccgcctcc gcaaacatcc accatggccc
     6901 gcatccggat cgagctgccc gagcgcttca ccttctccac cgacatcccg ctgctggtgt
     6961 cgcacatcaa ctacggcaac cacctcgaca acgcccaact gctcggcatc gtctccgagg
     7021 cgcgcgtgcg cttcctcaag tccatgggct attccgagct cgacatcgag ggcctcggca
     7081 tcatcgtcgc cgacgccgcg ctgcaatacc gctccgaggc cttccacggc gagaccatgc
     7141 gggtggagat gggcgc
//
LOCUS       DAOQNN010000145         7116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5651_length_7116_cov_8.322476, whole genome shotgun sequence.
ACCESSION   DAOQNN010000145 DAOQNN010000000
VERSION     DAOQNN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5651_length_7116_cov_8.322476"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PKV97_09135"
     CDS             <1..866
                     /locus_tag="PKV97_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB06077.1"
                     /translation="RLRAGFGSERLRQLAQPVAREVPHDALVLPDDLLERFAALVARC
                     RQRETLSDGLGSSLAEPARGVRALFCGESGAGKTLAASRLATILGAPLFRVDLSAVMN
                     KYIGESEKNLGRLLDEAAALDVILLIDEADALFGRRSEGKETGERYANMLTNFLLTRI
                     EQHPGIVVLTSNNRGRIDSAFTRRFDVIIDFPPPGANERLRLWESHLGGRAPDAALCR
                     QLASYCTLPGGYIRNAVLQAAALDPVGDAGAGAPARPIAAERLVAALLEEYRKIGRTP
                     PPQLLNARSAP"
     gene            863..3148
                     /locus_tag="PKV97_09140"
     CDS             863..3148
                     /locus_tag="PKV97_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNB06078.1"
                     /translation="MNHPTATAQRLAPADKTARAHKSPDSPPASAATVPASRQDHAGE
                     AVTGPTPQARPGGSAAGKRPGAPGYLQARLAVSQPQDAEEREADRIAGEVSRALRTPA
                     RSASGRECRDCSGEAPHGSLEPAITPPRKPTLAPRLMRETRPGSAAGTAPASATKGAD
                     AGPHHVPVATERRIAARRGQGAPLDPGLRAQLETRLGRDLSAVRIHTDAEADSMCASM
                     QARAFTVGNDVYFCAGSYAPASDTGLELLAHELAHVGQQADAGGPQSAAPKLQRDFWD
                     TLFGDSRAPVDPMAGCRRELDASAQWATAGPYPAAPRGIVGAGGIGGFDAQYRADPLA
                     GDGVLAIEQGVAAQFKDTLVRNAGVIAPHPDLPATPEIRTLASRMNAIPVAVVRNALL
                     ARYQWTPAEQAPWLVRLESLIETTWGGRHSFFLNRPRWNWLGAEVRVDLDIGQRAKAA
                     TDHLVVEIYKTPPGESLRSFDISHEVGSGSTTDPADQTMRLASTTAGPKEYDLLRQSV
                     EFDYDSDALTGTAIATLNGFIARFNGAVGNAAHQEVRVEIVGHTSAAGSEHYNVDLSR
                     RRAEAVRTHLERHGFANTASRVHIRARGEAEADPTQPRRASDQRADLVVDGGGRMVTA
                     AHEWGHAFGLDDEYVTGATRVGDPVDHDAMTRAMTDANGVHLPGAIKEHNGGIMSFGN
                     EVRPQHYATFHHALASVTAQSPWALGLHKPKWQVAMECGAPSPPGDWPVPAPARPVPG
                     AGPANPVPGTTPGNDDGSAVA"
     gene            3264..6530
                     /locus_tag="PKV97_09145"
     CDS             3264..6530
                     /locus_tag="PKV97_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNB06079.1"
                     /translation="MPAYARPPARVSQPGENIEREAEAHAAAVAPTNSPHPTPDSAPA
                     PVPTRSTEDSAGASSAANVGSTPPPASTSASASADANSPSARLPAYAAGRVEARRGRG
                     DPLPAATRAPLEDHFGRDFADVRLHSDAEAARLTAALGARAFTSGRDIYLAPDTVASD
                     TEEGRHLLAHELAHVVQQDGQAAATLARAIEPPPPARYHETERSAAALAALDRLEIPA
                     VKARHLPLYSGLAQAHQLKRVRGYGREGADQRSVWSRQVEVGADAVRERLTERGIPVP
                     ADPSGRVRLKLHRGARTTSKRLSELQTLLRIPEWDREGRRRDFQVDHIVELQVSGQMG
                     SGVGNSVENMELLDQPSNSSSGGTIRSSIYRKLDGFLDTLEPRPDRASFLRGHDLVFT
                     SVAATGAGSAATGSAWWTRAEIEQAHTLGTASAPTAAEDGDRAGSAEEFILSAAPGGI
                     AVGRFRHSPGAAPTIGATQARALAGLRITAIALTDLTGTPDGPVGTLSAEWDLPADWQ
                     PANPAITISLQGDGEYRGYPSALPGLDLEYRHLSPVSFTRISTEDGELYAEGTLTPSI
                     PILAAPLTVQLRGRELGFALDYGPEQVSLPIPRTTIDDAWVSVFYSTTRGLGVGGDIL
                     YSIEGAGSGELSASVSTGGGVAFEGGFTFDPALFDRARVRAWWRDGRLGAEGTIGIDT
                     PDKIRGIRSATASVRVDEGRWSFNGSAELSVPGLSQASIAIRQGEGGLELAGDVALAT
                     NPAIRSGTLHVECAQTDGEWKVAASGTAQPAIPGVDAELAVTYADGAFDARFSGAFRR
                     GMLSGQLSVGATNRAVAADGSPGGPPSAPDAPIVVYGSGSATVRIAPWLQGSAGLRVA
                     PDGELTVSGEIALPASLEIFSRLEYDKRLFGMSTQIPIVPGVVAEVGGNLSANASVGP
                     GALDRLAVRIEYNPAHEDDTHVSGEAHLEVPAQAGLRLGARAGVGLGITGASATGGLE
                     IGGALGIAGAAEAGVRIDWMPSRGLEIDAEAALHAQPRFRFDVSGYVAVTVLGASLYD
                     ERIELAAYELGSGLEFGVRFPVTYREGEPFDLSLDDLEFQVPEVDPAALISQLGETIF
                     "
     gene            6760..>7116
                     /locus_tag="PKV97_09150"
     CDS             6760..>7116
                     /locus_tag="PKV97_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNB06080.1"
                     /translation="MDTHYTATAKLLHWLIALMIFGMLGFGFYMTGLDLSPTKLELYS
                     WHKWAGVTVFLLVLVRLAWRIGHRPPALPAHMPAIERLAAHAGHHLLYLLMLAIPLSG
                     WLMSSAKGFQTVWFGVL"
BASE COUNT         1087 a         2797 c         2317 g          915 t
ORIGIN      
        1 accggctgcg cgccggcttc ggcagcgagc gcctgcgcca gctcgcgcag ccggtggcac
       61 gcgaagtgcc ccacgatgcg ctggtgctgc ccgacgacct gctcgagcgt ttcgcggccc
      121 tggtcgcgcg ctgccgccag cgcgaaacgc tctcggacgg cctcggcagc agtcttgccg
      181 agccggcacg cggcgtgcgg gcgctgttct gcggcgagag cggagccggc aagacgctcg
      241 ctgcaagccg gctggcgacc atcctcggcg caccgctctt ccgcgtggac ctgtccgcgg
      301 tgatgaacaa gtacatcgga gagtccgaga agaacctcgg acgcctgctg gacgaagccg
      361 cggcgctgga cgtgatcctg ctgatcgatg aagcggacgc cctcttcggc cgccgcagcg
      421 agggcaagga gaccggcgag cgttacgcca acatgctcac caacttcctc ctcacgcgca
      481 ttgaacagca cccaggcatc gtcgtgctga cgagcaacaa tcgcggccgc atcgacagcg
      541 ccttcacccg tcgcttcgac gtgatcatcg attttcctcc gccgggcgcg aacgaacgcc
      601 tgcgcctctg ggagtcgcat ctcggaggac gcgcccccga cgccgcgctt tgccgccagc
      661 ttgccagcta ctgcacgctc cccggcggct acatccgcaa cgccgtgctg caggcggccg
      721 cgctcgatcc ggtcggggac gcgggcgccg gcgccccggc ccgcccgatc gccgcggaac
      781 ggctggtggc ggcgctgctc gaggagtacc gcaagatcgg ccgcacacct ccaccccagc
      841 tcctgaacgc acggagtgcg ccatgaacca ccccaccgcc accgcgcaac gcctcgcgcc
      901 ggcagacaag acggctcgtg cccacaagtc gccggacagc ccccccgcgt ccgcggccac
      961 cgttcctgcc agccgacagg accacgccgg cgaggccgtg accgggccga ccccgcaagc
     1021 ccgacccggc ggcagcgccg ccggcaaacg cccgggtgcg cccggctacc tgcaggcccg
     1081 gctcgccgtc agccaaccgc aggacgccga ggagcgcgag gccgaccgca tcgcgggcga
     1141 ggtctcccgc gccctgcgca cgccggcccg cagcgcttcc gggcgggagt gcagggactg
     1201 cagcggcgag gctccgcacg ggagcctcga accggcgatc accccaccgc gaaagccgac
     1261 cctcgccccc aggctgatgc gcgagacccg gccagggagc gctgcgggga ctgccccggc
     1321 ttccgccacg aagggtgcgg acgcgggccc ccatcacgtg cccgtcgcga ccgaacgacg
     1381 catcgccgca cgccgcggcc agggcgcgcc cctcgacccc ggcctgcggg cacagcttga
     1441 gacccgactc ggtcgcgacc tgtccgcggt gcggatccac accgacgccg aagccgactc
     1501 gatgtgcgcc agcatgcagg cacgcgcctt cacggtcggc aacgacgtct atttttgcgc
     1561 cggcagctac gcccctgcga gcgacaccgg cctcgagctg ctcgcccacg agctcgccca
     1621 cgtcggccag caggccgatg ccggcggccc gcagagtgcc gcgcccaagc tgcagcgcga
     1681 tttctgggac acgctcttcg gtgacagccg cgcacccgtc gatccgatgg cgggctgccg
     1741 tcgagagctc gacgcgtcgg cgcaatgggc gacggccggc ccctaccctg cagcgccccg
     1801 gggcatcgtc ggcgcgggcg gcatcggcgg cttcgatgcc cagtaccgtg ccgacccgct
     1861 cgcgggcgac ggcgtgctcg cgatcgagca aggcgtggcc gcacagttca aggacaccct
     1921 ggtgcggaac gcgggcgtga tcgcgccgca ccccgacctc cccgccacgc cggagatccg
     1981 cacgctcgcc agccggatga acgcgatccc ggtcgccgtg gtgcgcaatg cgctgctcgc
     2041 acgctaccag tggacgcctg cggagcaggc gccctggctc gtccgcctgg aatcgctgat
     2101 cgagacgacc tggggcggcc ggcacagctt cttcctcaac aggccacgat ggaactggct
     2161 cggtgccgaa gtgcgcgtgg acctcgacat cggccagcgc gcgaaggcgg ccaccgacca
     2221 cctcgtcgtg gagatctaca agaccccgcc aggcgagagc ctgcgcagct tcgacatctc
     2281 gcacgaggtc ggttcgggca gcacgaccga ccctgccgac cagacgatgc ggctcgccag
     2341 caccacggcc ggccccaagg agtacgacct gctgcgccag tccgtcgagt tcgactacga
     2401 ctcggacgcg ctcacgggca ccgcgatcgc aacgctcaac ggcttcatcg cacgcttcaa
     2461 cggtgcagtc ggcaacgctg cccaccagga ggtgcgcgtc gagatcgtcg gccacaccag
     2521 cgccgccggc agcgagcact acaacgtcga cctctcgcga cggcgcgccg aggccgtgcg
     2581 cacgcatctc gagcgccacg gcttcgcgaa caccgccagc cgtgtgcaca tccgcgcgcg
     2641 cggcgaggcc gaggccgatc cgacgcagcc ccgacgcgcc agcgaccagc gcgccgacct
     2701 cgtcgtcgat ggcggcgggc ggatggtgac ggcggcccac gagtggggac acgccttcgg
     2761 cctggacgac gagtacgtga ccggcgccac ccgcgtcggc gacccggtgg accacgacgc
     2821 gatgacccgg gcgatgaccg acgccaacgg cgtccatctg cccggtgcga tcaaggaaca
     2881 caacggcggc atcatgtcct tcggcaacga ggtgcgtccg cagcactacg cgaccttcca
     2941 tcacgcactc gccagcgtca ccgcgcagtc gccctgggcg ctgggattgc acaagccgaa
     3001 atggcaagtt gcaatggaat gcggcgcccc ctcgccaccg ggcgactggc cggtgccggc
     3061 accggcccgg ccggttcccg gcgccggccc cgccaacccg gtgccgggta ccaccccggg
     3121 caacgacgat ggaagcgccg tcgcctaagc tcgcgcaccg gcctgccgaa cggcccgcgc
     3181 cggccacccg acctgcagcc acgaccggcc gtgccggccc ggcggcaccc gcgacggccg
     3241 cgagccgcct gcctccaccg tccatgccgg cctacgcacg tccccccgca cgcgtgagcc
     3301 agcccggcga gaacatcgag cgcgaggccg aggcgcacgc cgccgccgtc gccccgacga
     3361 actcgcccca tccgacgccg gactcggccc ccgcgccagt accgacccgc agcaccgaag
     3421 acagcgcggg cgcgtcctcg gctgcgaacg tcggctccac ccccccaccc gcatccacgt
     3481 ccgcatccgc atccgccgac gcgaactcgc cctccgcgcg ccttcccgcc tatgccgccg
     3541 gccgtgtcga agcgcgccgc gggcgcggcg atccgctgcc ggccgctacc cgcgccccgc
     3601 tcgaggacca cttcggtcgc gacttcgccg acgtgcgcct ccattcggac gccgaagcgg
     3661 cgcgcctcac cgctgcgctc ggcgcacgcg cgttcacgtc ggggcgcgac atctacctcg
     3721 caccggacac cgtcgcctcc gacaccgagg aggggcgtca cctcctcgcg cacgaactcg
     3781 cccacgtcgt gcagcaggat ggccaggccg cggcgacact cgcgcgcgcg atcgaaccgc
     3841 cccctccggc ccgctaccac gagaccgagc gcagcgctgc cgccctcgct gcactggatc
     3901 gcctcgagat ccccgcggtg aaggcgcgcc acctgccgct ctacagcggc ctcgcgcagg
     3961 cgcaccagct caagcgcgtg cgtggctacg gacgcgaggg cgccgaccag cgctcggtat
     4021 ggagccgcca ggtcgaggtc ggcgccgacg ccgtgcgcga acgcctgacc gaacgcggca
     4081 tcccggttcc cgccgacccc agcggccgcg tacgcctgaa actgcatcgc ggcgcacgta
     4141 cgacgtcgaa acgcctgtcc gagctgcaga ccctgctgcg catccccgaa tgggaccgcg
     4201 agggccgcag gcgcgatttc caggtcgacc acatcgtcga actgcaggtc tccggccaga
     4261 tgggctcggg ggtgggcaac agcgtcgaga acatggagct cctcgaccag ccctcgaact
     4321 cgagctcggg cggcacgatc cgcagcagca tctatcgcaa gctcgacggc ttcctcgata
     4381 ccctggagcc tcgacccgac cgagcaagct tcctgcgcgg acacgacctc gtcttcacca
     4441 gcgtcgcagc aaccggcgcg ggctcggcgg cgacgggctc ggcgtggtgg accagggccg
     4501 agatcgagca ggcccacacc ctcggcaccg cgagcgcccc caccgccgcc gaagacggcg
     4561 accgcgccgg cagcgccgag gagttcatcc tcagcgccgc ccccggcggc atcgccgtgg
     4621 gccgcttccg ccacagcccc ggtgccgcgc ccacgatcgg cgccacccag gcgcgcgcgc
     4681 tcgccggcct gcgcatcacg gcgatcgcgc tcaccgacct taccggcacg cccgacggcc
     4741 ccgtcggcac gctctcggca gaatgggacc tgcccgccga ctggcaaccc gccaatcctg
     4801 cgatcacgat ctcgctccag ggcgatggcg agtaccgcgg ctacccgtcg gcgcttcccg
     4861 gcctcgatct cgagtaccgc catctcagcc cggtgagttt cacccgcatc tccaccgaag
     4921 acggcgagct gtacgccgag ggcacgctca cgccctcgat ccccatcctc gcagctccgc
     4981 tgacggtgca actgcgcgga cgcgagctcg gcttcgcgct cgactacggt ccggaacagg
     5041 tcagcctgcc gatcccgcgc acgacgatcg acgacgcctg ggtcagcgtc ttctattcga
     5101 ccacccgcgg actgggcgtg ggaggcgaca tcctgtattc gatcgagggg gcaggcagcg
     5161 gcgagctcag cgcctcggtc tcgaccggcg ggggcgtcgc cttcgagggc ggcttcactt
     5221 tcgatcccgc cctcttcgac cgcgctcgcg tccgcgcctg gtggcgtgat ggtcgcctgg
     5281 gcgccgaagg cacgatcggc atcgataccc cggacaagat ccgtggcatc cgcagcgcga
     5341 ccgcctcggt ccgtgtcgat gaagggcgct ggtcgttcaa cggcagtgcc gaactctccg
     5401 tgcccggtct cagccaggcc tcgatcgcga tccgccaggg cgagggcgga ctcgagctcg
     5461 cgggcgacgt cgccctcgcc accaaccccg ccatccgctc cggcaccctg cacgtcgaat
     5521 gcgcgcagac cgacggcgag tggaaggtcg cggcgagcgg caccgctcag cctgcgatcc
     5581 ccggcgtcga cgcggagctc gccgtcacct atgcggacgg cgccttcgac gcgcgcttct
     5641 ccggcgcctt ccgtcgcggc atgctctccg gccagctcag cgtcggcgcc accaaccgcg
     5701 ccgtcgctgc ggacgggagc ccgggcggcc cgccaagcgc tccggatgcg cccatcgtgg
     5761 tctacggcag cggctccgcg accgtgcgga tcgccccctg gctgcagggc agcgcgggcc
     5821 tgcgggtcgc tcccgacggc gagctcaccg tgtccggcga gatcgccctg cccgcttcgc
     5881 tggagatctt ctcccggctg gagtacgaca agcgcctgtt cggcatgtcg acgcagatcc
     5941 ccatcgtccc cggcgtggtc gccgaggtcg gcggcaatct cagtgccaac gccagcgtcg
     6001 gccccggggc actggaccgg ctcgctgtcc gcatcgaata caaccccgca cacgaggatg
     6061 acacccacgt cagcggcgag gcccacctcg aggttccggc gcaggcaggc ctgcggctcg
     6121 gcgcgcgtgc cggcgtcggc ctcggcatca ccggcgccag tgccaccggg ggcctcgaga
     6181 tcggcggcgc cctcggcatc gcaggggctg ccgaggctgg cgtgcgcatc gactggatgc
     6241 cctcgcgcgg cctcgagatc gacgccgagg ctgcgctcca cgcccaaccg cgcttccgct
     6301 tcgacgtttc cggctacgtc gccgtcaccg tgctcggcgc cagcctctac gacgagcgca
     6361 tcgagctcgc cgcctatgag ctcggctctg gcctcgagtt cggcgtccgc ttccccgtca
     6421 cctaccgtga gggagaaccc ttcgacctct ccctcgacga cctcgagttc caggtccccg
     6481 aggtggatcc ggcggcgctg atcagccagc tcggggagac gatcttctga ttcgcggaga
     6541 gggcgggagg tgaaagcgac ccgaccccat cccccgctcg gagggagcga atcgcgcaac
     6601 ccgagacccg gcctcagcgg aaaacaggct caattgttgc caaatcagca actaaccttt
     6661 gcccgacttc ggctgtttaa gccttgtgga tgagtctaaa gtgcaaccaa cacgaacatt
     6721 tggccggcgc ccccgatcga cggccgaagg accaagcaca tggacacgca ctacaccgcc
     6781 accgccaagc tgctgcactg gctcatcgcg ctgatgatct tcggcatgct cggattcggc
     6841 ttctacatga cgggtctgga cctgtcgccc accaagctgg aactctactc ctggcacaag
     6901 tgggcgggcg tgacggtgtt cctgctcgtg ctggtgcgcc tggcctggcg gatcggccac
     6961 cgcccgccgg cgctgcccgc gcacatgccg gccatcgagc gcctggccgc gcatgcgggc
     7021 caccacctgc tgtacctgct gatgctcgcg atcccgctca gcggctggct gatgagctcg
     7081 gccaagggct tccagacggt gtggttcggc gtgctg
//
LOCUS       DAOQNN010000146         7095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5678_length_7095_cov_9.340199, whole genome shotgun sequence.
ACCESSION   DAOQNN010000146 DAOQNN010000000
VERSION     DAOQNN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5678_length_7095_cov_9.340199"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PKV97_09155"
     CDS             <1..340
                     /locus_tag="PKV97_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HNB06081.1"
                     /translation="GKLTKIAQGLSVTHAWREEVDRALIAEAAQEVGAPRALVAEIRA
                     AETARFAAERLSALGLAVAFHRALAGRAIRSLRQRYPGPHRLTVLACNFEGVPIVSVD
                     EADLKETTHA"
     gene            333..1649
                     /locus_tag="PKV97_09160"
     CDS             333..1649
                     /locus_tag="PKV97_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cobalt-precorrin-7
                     (C(5))-methyltransferase/cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HNB06082.1"
                     /translation="MPESTAPVCSIVGLLDDGWDGLGAAARRRIEAAASLIGAGRTLE
                     RIAPHAPSARLLNMDGALTRVPGWIEEAVLEGDAAVVLATGDPLCHGIAGFLIDKLGA
                     ARLEVLPATSTLQLAFARLKRSWQEARFVSCHGADADEWQADPRQPGPTPEHGLYRVV
                     RAVAEHPLVACFTSPANSPDRIARALLAAGHGAPGCDEDLRLSVVARLCLDDEAVFAG
                     LSLQEAASRRFPEPNVVVIERTCVGTALFGVDDLDYVQRTPEKGLITKLEARAVALAK
                     LALRADSRVWDIGAGSGSVGLEASRIARHGHVWAIEKNPADAANARTNARRLRASNYS
                     LFEGKAPAGLDAWPDPDAVFIGGSGGELGELITLVLRRLVPGGRLVMNFVTLENLATA
                     TSVLAAADTAWEVTMLSAARSQPILDMHRLAAQNPVWIVTTRKEAP"
     gene            1646..2524
                     /gene="cobI"
                     /locus_tag="PKV97_09165"
     CDS             1646..2524
                     /gene="cobI"
                     /locus_tag="PKV97_09165"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HNB06083.1"
                     /translation="MNPHPANTARFGRLIGISLGPGDPELVTRRGWAALQSDARWAYP
                     VKKAEESSYALDIVRRGGLSIPADAVELVFPMTRDAVALAKAWARAATQVVALLAEGR
                     DLAFLVEGDASTYSTFRHLARTVRELAPEVEVDTIPGVSSFAAAAAVADLALAEEDET
                     MAVIPAAYGTTVIDHLLDEFDTLVLMKVKPLLDEVLDLLERRGLLATSCFVEKVGAPA
                     QRVVHDLASLRGAQVNYLSLLLVQNPKRARGELRRGCRKRAAQVEPQATAQAPSPDTG
                     GAAAHPGESPAATALS"
     gene            2536..3291
                     /locus_tag="PKV97_09170"
                     /pseudo
     CDS             2536..3291
                     /locus_tag="PKV97_09170"
                     /inference="COORDINATES: protein motif:HMM:NF023189.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis central domain-containing
                     protein"
     gene            3304..3741
                     /locus_tag="PKV97_09175"
     CDS             3304..3741
                     /locus_tag="PKV97_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HNB06084.1"
                     /translation="MAAAKKLALGLGCDRGTPAATITRAIDAALARIGASRIDVAAVA
                     SITLKADEAGLLEAATAAGWTLRFYPPAVLAAVDVPNPSETVRRYTGTPSVSEAAALL
                     AAGTALATDPGALRVEKHKLRGQDGRNATVSIARLPASSPAPN"
     gene            3825..4811
                     /gene="cobJ"
                     /locus_tag="PKV97_09180"
     CDS             3825..4811
                     /gene="cobJ"
                     /locus_tag="PKV97_09180"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HNB06085.1"
                     /translation="MNAPETIVPPGTSAPAGKIMLVGLGPGAHAHLTERARAAIAEAD
                     TIIGYVTYIRLVADLLEGKEVIRKSMTEELDRAIEALERARQGRKVALVSSGDAGVYG
                     MAGPTFEVLFQAGWTPPSGKPGDIEVEIVPGASALNTCAALVGAPLTHDFCAISLSDL
                     LTPWPAIARRLDAAAYADFVVALYNPKSGRRTGQIVEAQRIFLRHRDPATPVAIVKSA
                     YRPRQRIEFSTLERMAEADIGMLTTVLIGNSNTFVRDGLMVTPRGYANKYAVDGSERA
                     TRAGEQAGRSLSSGLDGWLATIRASGRNARELAAEYRLPEDYIRAALAHPPA"
     gene            4900..6198
                     /locus_tag="PKV97_09185"
     CDS             4900..6198
                     /locus_tag="PKV97_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNB06086.1"
                     /translation="MSKLQALYDSKRMTASDAAGQVRDGDTVVVPTGVGEPPALLHAL
                     SARRHALRDVAVSQILPLRKFAYLDPETRANVRHDAYFFGGANRAGGQAGWVDYVPAY
                     FSELPMLIERGLSPADVVVSLASPMDEHGYFSLSLAPDYTMAAIRRARVVLLEVNPNV
                     PFANGDCLVHISRVAGLVESDDELFEVGLPEIGPVQQAIGKYVAELIDDGSTLQIGYG
                     GIPDAVVVQLQHKRELGIHTEMLGDGILSLIESGAVTNRKKTFMPGRTIATFALGSRR
                     LYRTMHRNPGIEMHPVEFTNDPYLAARNDKLCAINATMQIDLIGQCGSESLGHQPYSG
                     TGGQADFVRAANRSKDGKAFIVLPSTAKNGTVSRIAPVLSPGTHVTTSKNDINYVVTE
                     YGVAQLRGKTAKQRAEALIGIAHPDFRGELRDAARKMNLL"
     gene            complement(6350..6784)
                     /locus_tag="PKV97_09190"
     CDS             complement(6350..6784)
                     /locus_tag="PKV97_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB06087.1"
                     /translation="MPSRPIHQILSDRPFVGVSADTPVREVVRQMNALQRSAALVTEH
                     GVLTGIFTERDAAFGVLAAGLDADTTPVGAVMTHNPVTLTEDRPFGHALHLMYENGVR
                     HVPIVDANRRPLGVVTARDALQLDALTFGAELVRREEITVIL"
BASE COUNT         1043 a         2726 c         2367 g          959 t
ORIGIN      
        1 cggcaagctc accaagatcg cccagggcct gtcggtgacc catgcctggc gcgaggaggt
       61 cgaccgtgcg ctgatcgccg aggccgcgca ggaggtcggc gccccgcgcg cactcgtcgc
      121 cgagatccgc gccgccgaga ccgcccgctt cgccgccgaa cgcctgagcg cgctcggcct
      181 ggccgtggcc ttccaccgcg cgctcgccgg gcgcgccatc cgcagcctgc gccagcgcta
      241 ccccggcccg caccggctca ccgtgctggc gtgcaacttc gagggcgtac cgatcgtgag
      301 cgtcgatgag gccgacctga aggagaccac gcatgcctga atccactgcc cccgtctgca
      361 gcatcgtcgg cctgctcgac gacggctggg acggcctcgg cgccgcagcg cgccggcgca
      421 tcgaagccgc cgccagcctc atcggcgccg gacgcacgct ggaacgcatc gcaccgcacg
      481 cccccagcgc ccgcctgctg aacatggacg gcgcgctcac acgcgtgccg ggctggatcg
      541 aggaggccgt gctcgagggc gacgccgccg tggtgctcgc caccggcgac cccttgtgcc
      601 atggcatcgc cggcttcctg atcgacaagc tcggcgccgc gcggctcgag gtgctgcccg
      661 caacctcgac gctgcagctc gccttcgcgc gcctgaagag gtcgtggcag gaggcccgct
      721 tcgtgtcttg ccacggcgcc gacgccgacg aatggcaggc cgatccgcgg caacccggcc
      781 ccacgccgga gcacggcctg taccgcgtgg tccgcgccgt cgccgaacac ccgctggtcg
      841 cctgcttcac cagccccgcc aacagccccg accgcatcgc ccgcgccttg ctcgcggctg
      901 gtcatggcgc gccgggctgc gacgaggacc tgcgcctgtc ggtggtcgcc cgcctgtgcc
      961 tggacgacga agcggtgttc gccgggctga gcctgcagga ggccgccagc cggcgcttcc
     1021 cggaacccaa cgtcgtggtg atcgagcgca cgtgcgtcgg taccgcgctg ttcggcgtcg
     1081 acgacctcga ctatgtgcag cgcacacccg agaagggcct gatcaccaag ctcgaggcac
     1141 gcgcggtcgc gctggccaag ctcgcgctgc gcgccgacag ccgggtgtgg gacatcggcg
     1201 ccggttccgg ctcggtcgga ctggaggcca gccgcatcgc ccgccacggc cacgtatggg
     1261 cgatcgagaa gaaccccgcc gacgccgcca atgcacgcac caatgcccgt cggctgcgcg
     1321 ccagcaacta cagcctgttc gagggcaagg cccccgccgg cctggatgcc tggcccgacc
     1381 ccgatgcggt gttcatcggc ggctcgggcg gcgaactcgg cgagctgatc accctcgtcc
     1441 tgcggcggct cgttcccggc ggccggctgg tgatgaactt cgtcaccctc gagaacctcg
     1501 ccaccgccac ctccgtgctg gccgcggccg acaccgcctg ggaggtgacg atgctctcgg
     1561 ccgcgcgcag ccaacccatc ctcgacatgc atcggctcgc cgcgcagaac ccggtgtgga
     1621 tcgtcaccac ccgcaaggag gctccatgaa tccgcatccc gcgaacacgg ctcgcttcgg
     1681 ccgcctgatc ggcatctcgc tcggccccgg cgaccccgag ctcgtcaccc gccgcggctg
     1741 ggccgcgctg cagtcggacg cacgctgggc gtatccggtg aagaaggcgg aggaatcctc
     1801 ctacgcgctc gacatcgtcc gccgcggcgg tctgtccatc cccgcggacg cggtggagct
     1861 cgtctttccg atgacgcgcg acgcggtcgc actcgccaag gcctgggcgc gcgccgcgac
     1921 gcaggtggtg gcgctgctcg cggaaggccg cgacctcgcc ttcctggtcg agggcgacgc
     1981 ctccacctat tccaccttcc gccacctcgc gcgcacggtg cgcgagctcg cgcccgaggt
     2041 cgaggtcgac accatccccg gggtcagttc cttcgccgcc gccgcggcgg tggccgacct
     2101 ggcgctggcc gaagaggacg agacgatggc ggtgatcccc gccgcctacg gcaccaccgt
     2161 catcgaccac ctgctcgacg agttcgacac cctggtgctg atgaaggtga agccgctgct
     2221 cgacgaggtg ctcgacctgc tcgagcgccg cggcctgctc gccacgagct gcttcgtcga
     2281 gaaggtgggg gcgcccgcgc agcgcgtggt gcacgacctt gccagcctgc gcggcgcgca
     2341 ggtcaattac ctctccctgc tgctggtgca gaaccccaag cgcgcgcgcg gcgagctgcg
     2401 ccgcggctgc cgcaagcgcg ccgcgcaagt ggaaccgcaa gccaccgcgc aggccccctc
     2461 cccggacacc ggaggcgccg ccgcgcaccc tggcgagtcc cccgcggcca cggccctttc
     2521 ctgaggttcc gcacgatgcc ttcgccgccc ctccagcctc tgcccttccc ctgcacccgc
     2581 atcgccgtgg tgtcgatcac ccgccacggc atcgcgctcg ccggtcgcgt catcgccgcc
     2641 ttgcccggcg ccacgctgtt cgcaccgcgg aagttcgccg ccgaggccgc cgcagccgca
     2701 cccggcgcct tcgcctgcta cgagggcaag gtgggcgacc aggtgccggc gctgttcgcc
     2761 ggcttcgacg gcatcgtcgc catcgtgtcg ctcggcgcgg tggtgcgcct gatcgcgccg
     2821 catctcggca acaaggagac cgaccccggc gtggtggtgc tcgacgaggc cgggcgctgg
     2881 gcgatcccgg tgctgtccgg ccacctcggc ggcgccaacg cgatcgccgc cgcgctgcag
     2941 caggcggtgg gcgccacgcc ggtgctgacc accgcatcgg acgcgcgcgc gacgctggcg
     3001 gtggacctgc tcggccgcga gctcggctgg accttcgaag ccagccacga cgagatcgtg
     3061 cgcgccagcg ccgcggtggt gaacgacgag ccggtggcgc tggtgcagga gaccggcagc
     3121 accgactggt ggtcgcgcca cgccaacggc cgcagcggcc cgctgccggc gaacatccgg
     3181 cgcttcgcgc gcctggaaga cgtcgagccc gaacacttcg gcgccgtgct gtggatcagc
     3241 gaccgccctc tgcccaccgg ctacgctgca cgcctcgccg gcaagcgggt gatctaccgc
     3301 cccatcgctg cggcgaagaa gctcgcgctc ggcctcggct gcgaccgcgg cacgccggcg
     3361 gcgaccatca cgcgagcgat cgacgcggcg ctcgcccgca tcggtgcgtc gcgcatcgac
     3421 gtcgccgccg tggccagcat cacgctgaag gccgacgaag ccggcctgct ggaggcggcc
     3481 accgccgccg gctggacact tcggttctac ccaccggcgg tgctcgccgc ggtcgacgtg
     3541 cccaacccgt cggagacggt gcgccgatac accggcacgc cctcggtctc cgaggccgca
     3601 gccttgctcg ctgccgggac cgctctcgcc accgacccgg gcgcgctgcg cgtcgaaaag
     3661 cacaagctgc gcggccagga cggccgcaac gccaccgtct ccatcgcccg ccttcccgcc
     3721 tcctcgcccg ccccgaactg acgaccaccc cgagcacccg gcaccaccgc cggggctcga
     3781 cgcgcgccct cgcgggccgc gtttcgcacc ggagtccctc ccgcatgaac gcccccgaaa
     3841 ccatcgtccc tcccggaacg tccgcccccg ccggcaagat catgctcgtc ggcctcggcc
     3901 cgggcgcgca cgcccacctc acggagcgcg cgcgcgccgc gatcgccgag gccgacacga
     3961 tcatcggcta cgtcacctac atccgcctcg tggccgacct gctcgagggc aaggaggtca
     4021 tccgcaagtc gatgaccgag gagctcgacc gcgccatcga ggcgctcgag cgcgcccgcc
     4081 agggccgcaa ggtggcgctc gtctcctcgg gcgacgccgg ggtgtacggc atggcgggcc
     4141 cgaccttcga ggtgctcttc caggccgggt ggacaccgcc gagcggaaag cccggcgaca
     4201 tcgaggtcga gatcgtgccc ggcgcctcgg ccctcaacac ctgcgccgcg ctcgtcggcg
     4261 cgccgctgac gcacgatttc tgcgcgatct cgctgtccga cctgctcacg ccctggccgg
     4321 ccatcgcacg ccggctcgac gccgccgcct atgccgactt cgtggtcgcg ctctacaacc
     4381 ccaagagcgg ccgccgcacc ggccagatcg tcgaggcgca gcgcatcttc ctgcgccatc
     4441 gcgacccggc cacgccggtg gcgatcgtca agtccgccta ccggcccagg cagcgcatcg
     4501 agttcagcac cctcgagcgc atggccgagg ccgacatcgg catgctgacc acggtgctga
     4561 tcggcaactc caacaccttc gtgcgcgacg ggttgatggt gaccccgcgc ggctacgcca
     4621 acaagtacgc ggtcgacggc agcgagcgcg ccacccgcgc gggcgaacag gccgggcgct
     4681 cgctgtcgag cgggctcgac ggctggctgg cgacgatccg cgccagcggg cgcaacgccc
     4741 gggagctggc cgccgaatac cgcctgcccg aggactacat ccgcgccgcc ctcgcccacc
     4801 cacctgcttg agcgcgcacc ctttaccata cgactccgta tggaataccc gtataatcga
     4861 cttccttcga cgccagacca cacgcccggg agaaccgcga tgtccaagct gcaggccctc
     4921 tacgacagca agcgcatgac cgcctccgat gccgccggcc aggtccgcga cggcgacacc
     4981 gtggtcgtgc ccaccggcgt gggcgagccg ccggcgctgc tgcacgccct gtccgcgcgc
     5041 cgtcacgcgc tgcgcgacgt cgcggtgagc cagatcctgc cgctgcgcaa gttcgcctat
     5101 ctcgacccgg aaacgcgcgc gaacgtgcgc cacgacgcct acttcttcgg cggtgcgaac
     5161 cgcgcgggcg gccaggccgg ctgggtggac tacgtgccgg cctacttctc cgagctgccg
     5221 atgctgatcg agcgcggcct gtcaccggcg gacgtggtgg tgtcgctggc ctcgccgatg
     5281 gacgagcacg gctacttcag cctctcgctc gcgcccgact acaccatggc ggcgatccgg
     5341 cgcgcgcgcg tcgtgctgct cgaggtgaat ccgaacgtgc ccttcgccaa cggcgactgc
     5401 ctggtgcaca tctcccgggt cgccggcctg gtggagagcg acgacgagct cttcgaggtc
     5461 ggcctgcccg agatcggccc ggtgcagcag gcgatcggca agtacgtcgc cgagctcatc
     5521 gacgacggct ccacactgca gatcggctac ggcggcatcc ccgacgcggt ggtggtgcag
     5581 ttgcagcaca agcgcgagct cggcatccac accgagatgc tcggcgacgg aatcctgtcg
     5641 ctgatcgagt ccggcgcggt caccaaccgc aagaagacct tcatgccggg ccgcacgatc
     5701 gccaccttcg cgctcggctc gcggcgcctg tatcgcacca tgcaccgcaa tccgggcatc
     5761 gagatgcacc cggtggagtt caccaacgac ccctacctgg ccgcacgcaa cgacaagctg
     5821 tgcgcgatca acgccaccat gcagatcgac ctgatcggcc agtgcggctc ggagagcctg
     5881 ggccaccagc cctactcggg caccggcggc caggcggatt tcgtgcgcgc ggccaaccgc
     5941 tcgaaggacg gcaaggcctt catcgtgctg ccgtcgacgg caaagaacgg cacggtgtcg
     6001 cgcatcgcgc cggtgctgtc gccgggcacc cacgtcacca ccagcaagaa cgacatcaac
     6061 tacgtggtca ccgagtacgg cgtggcgcag ctgcgcggca agaccgcgaa acagcgcgcc
     6121 gaggcgctga tcggcatcgc gcacccggac ttccgcggcg aactgcgcga cgcggcgcgg
     6181 aagatgaacc tgctttgagg gggaggttgc gggcaggcgt gacaggcgat tcggcaagcg
     6241 atgcgcgtgg gtggatctcg accgcagcat tcgcgccttg cggcgctcct gcaagagccg
     6301 ccgcgtttcc ggccccctgt aggagcggcg caagccgcga acacggcgcc tacaggatga
     6361 ccgtgatctc ctcgcgccgc accagctcgg caccgaaggt gagcgcatca agctgaagcg
     6421 catcgcgcgc cgtgaccacg ccgagcgggc ggcggttggc gtcgacgatc ggcacgtggc
     6481 gcacgccgtt ctcgtacatc aggtgcaggg catgaccaaa ggggcggtcc tcggtcagcg
     6541 tcaccgggtt gtgcgtcatc accgcaccca ccggcgtggt gtcggcgtcc aggccggcgg
     6601 ccagcacgcc gaaggcggca tcgcgctcgg tgaagatgcc ggtgagcacg ccgtgctcgg
     6661 tgaccagggc ggcgctgcgc tgcagggcgt tcatctggcg gaccacctcg cgcaccgggg
     6721 tgtcggccga cacgccgacg aagggacgat cggaaaggat ctgatggatc ggacggctgg
     6781 gcatgatctg tctcctgatg ctcgttgttg gacggcaccc catccctagc ttcaatcgtg
     6841 ccagcttcgg aggtcggcgc cgggcattcc ggacggaacg ccaagccgct gttctagcat
     6901 gggattcttt cagcatcgtg accgcaggat gacgccaggg aatgcccgcc ggagccactc
     6961 ctggacacca tcacggtgca tcggcgagtg ctggcgcacc atcggagggc gcgcccgact
     7021 tcgcacgcgc catcggctcg cccatccgca ccgggccgcc cggcgcccag tcggagccga
     7081 agcgcagcac gtcct
//
LOCUS       DAOQNN010000147         7055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5740_length_7055_cov_10.326286, whole genome shotgun sequence.
ACCESSION   DAOQNN010000147 DAOQNN010000000
VERSION     DAOQNN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5740_length_7055_cov_10.326286"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PKV97_09195"
     CDS             complement(<1..877)
                     /locus_tag="PKV97_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HNB06088.1"
                     /translation="MNAQDVMKMIQENEVRFVDLRFTDIRGKEHHVGLPVSAFEEEHF
                     EHGHPFDGSSLAGWKGIQASDMILMPEPSTAYIDPFFDETTLVITCDVIEPSDGKGYD
                     RDPRSIAKRAEAYLKSTGIGDTAFFGPEPEFFIFDSVEWSVDMSGVYSKIISEEAAWS
                     TADKFEGGNTGHRPRVKGGYFPVPPVDSLNDVRAAMVLALEACGVPVEVHHHEVANAG
                     QCEIGTKFSTLTQRADWTQVLKYIVHNVAHQYGKTATFMPKPIVGDNGSGMHVHQSIW
                     KDGKNLFAGNGYAGLS"
     gene            complement(1096..2283)
                     /locus_tag="PKV97_09200"
     CDS             complement(1096..2283)
                     /locus_tag="PKV97_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNB06089.1"
                     /translation="MHSRQASSPVARPSAGADDLARSRGIQRATLVAIVTNTVLTIGQ
                     VVIGLFAGAFSLVADAAHTLSDLITDLLVLLAGRHSADPADTNHPYGHGRIETVTTLV
                     LGSVLAAVGVGFLWSSGIRLQNIGALPVLHPAALGMALLTLAAKEGLFRFSLAAARRL
                     NAPMLEANAWHARSDAASSLVVAAGIGGSLAGYPFLEPLAAAVVGFLILHMGIRLALK
                     SVRELIDTGLPEEELERLRTTIRETPGVIGLHEMRTRRMADRVLCDAHVQVDPRLTVS
                     EGHRVSDAVYLRVRAAHPEVRDVLVHIDPEDDGELQAVPPGPLPERDEILAHVRMLLG
                     PGFADPRRVQIHYLGQRVEVEIILPSLPDADALDALRSRRQTWLAEHPHYRNIRVFVE
                     LAP"
     gene            2343..2795
                     /locus_tag="PKV97_09205"
     CDS             2343..2795
                     /locus_tag="PKV97_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB06090.1"
                     /translation="MGTLSELLTLAHERAESLGLPYQGALTPAETWQVMQLAPGAKLV
                     DVRTRAELDWVGRVPGAIEIEWKSYPSMQENPNFLAQLKHQVDPEALVLFLCRSAVRS
                     DSAARLAGANGYANCYNVLEGFEGDKDANNQRNRIGGWRLAGLPWYQG"
     gene            2913..4013
                     /gene="nadA"
                     /locus_tag="PKV97_09210"
     CDS             2913..4013
                     /gene="nadA"
                     /locus_tag="PKV97_09210"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNB06091.1"
                     /translation="MQVSTISFDRFNVLADNEAQERIRAARARLGERAVLLCHHYQRA
                     DVYQHADLTGDSLKLARLASQTDAEYIVFCGVHFMAEVADILSKPSQIAILPDLAAGC
                     SMADMASLAKVERCWRELAEVLGKPDALITPVTYINSAADLKAFCGEHGGIVCTSTNA
                     PTILDWAFSKREKVLFFPDQHLGRWTGFKKGIPLDEMVVWDPDLEYGGLTPEQIRKAK
                     ILLWKGHCSVHQMFQPVHIDRWREKHPHGFVISHPESMLEVCQKSDYVGSTEFILNTI
                     TQAPPGTHWLVGTELNLVSRLAEEMKPQGKVVQFMAPTVCMCSTMQRIDPQHLAWTLE
                     NLAEGKVVNQIKVPAHEAELARIALDRMLAVS"
     gene            complement(4099..4530)
                     /locus_tag="PKV97_09215"
     CDS             complement(4099..4530)
                     /locus_tag="PKV97_09215"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNB06092.1"
                     /translation="MSALRPEVSIHTLNKAGGEYLPGYLGIEMLALDERRLNSRMPVK
                     KLHAAPNEFLHAASVVALADTTCGYATIAHLPAGADSFTTIELKSNHLGTVREGAIAC
                     SATAQHLGRTTQVWDATVTDEASGRVIALFRCTQMVLWPRG"
     gene            4640..5569
                     /locus_tag="PKV97_09220"
     CDS             4640..5569
                     /locus_tag="PKV97_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB06093.1"
                     /translation="MSWLPAWESFVRVVEGGSMAAAARTLGCTRAQVSKQVAELERRL
                     GAALFERSTRRLVLTPAGEVFLRHAARALEAVDSSEIAVRNLGERPSGTLRVSATLAF
                     GRHFVAPLVPVVTAANPGLEIELVLTDRLVDLVDDRIDLALRMTARPPEDAVARHLLR
                     LRWVLCAASAYVERHGSPREPAELAQHACIGHPAIGAAPWGLVAAAGAQARVAVRSRV
                     RFDSLDCTLDAVLAGHGIAILPAYLCEPALQAGSLRTVLDGWEPAFDVGRDLYACYTP
                     GRVGVSKVRVFMDALQQRLQPLPSWACAAGVGG"
     gene            5727..6476
                     /locus_tag="PKV97_09225"
     CDS             5727..6476
                     /locus_tag="PKV97_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNB06094.1"
                     /translation="MPLRICSYNIHKGFSQFNRRMVVHELRDRLRSLDADLVFLQEVQ
                     GLHLGHPARHPDWPALPQHEFLAEDAWQQIAYGGNAIYDHGHHGNAILSRHLILSTAN
                     QDVSDHRFERRGLLHCEVQLPEFDVPVHCVCVHLGLMAGSRRRQMEALAERMEELAPG
                     NAPLIIAGDFNDWRNRADDLLGRRLGLAEAFGSGRGRPARSYPSTLPFFRLDRIYVRG
                     FRVRQAQVHYGAPWAQISDHAALTADLEPIG"
     gene            6476..>7055
                     /locus_tag="PKV97_09230"
     CDS             6476..>7055
                     /locus_tag="PKV97_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNB06095.1"
                     /translation="MQFLAGNAVALLENGEQYFPALEAEIDRAEREIFLQSYIFEDDG
                     TGARIAAALARAAGRGVAVRVMVDGFGGRSFVSRLMPRLREAGVQVLIYRRELRTLSL
                     RRHRLRRLHRKVVAIDGRVAFVGGINVVDDFDAGALPHPRYDYAVRVEGPVLAPVVAS
                     AQRLWRLVAGASLRRRAAQPLLAPPLTDAAGSI"
BASE COUNT         1045 a         2412 c         2487 g         1111 t
ORIGIN      
        1 cggacaggcc ggcgtagccg ttgcccgcga acaggttctt gccgtccttc cagatcgact
       61 ggtgcacgtg catgccggag ccgttgtcgc cgacgatggg cttgggcatg aaggtggcgg
      121 tcttgccgta ctggtgggcg acgttgtgca cgatgtactt gagcacctgg gtccagtcgg
      181 cgcgctgggt cagcgtgctg aacttggtgc cgatctcgca ctggccggcg ttggccactt
      241 cgtggtggtg cacctcgacc ggcacgccgc aggcctcgag cgcgagcacc atggcggcgc
      301 gcacgtcgtt gaggctgtcg accggcggaa ccgggaagta gccgcccttg acgcgcggac
      361 ggtggccggt gttgccgcct tcgaacttgt cggcggtcga ccaggcggcc tcttccgaga
      421 tgatcttgct atacacgccc gacatgtcga ccgaccactc gaccgagtcg aagatgaaga
      481 actcgggctc ggggccgaag aaggcggtgt cgccgatgcc ggtgctcttc aggtaggcct
      541 cggcgcgctt ggcgatcgag cgcgggtcgc ggtcgtagcc cttgccgtcg gagggctcga
      601 tgacgtcgca ggtgatgacc agcgtggtct cgtcgaagaa ggggtcgatg tatgcggtcg
      661 acggctccgg catgaggatc atgtccgagg cctggatgcc cttccagccg gccagcgagg
      721 agccgtcgaa ggggtggccg tgctcgaagt gctcttcctc gaaggccgac accggcaggc
      781 cgacgtgatg ctccttgccg cgaatatccg tgaagcgcag gtccacgaag cgcacttcgt
      841 tttcctggat catcttcatg acgtcttggg cgttcatttt gactcctggt gagatgaggc
      901 gttgttgcgt tgagcgggtg gtgcagcggc ctggggtcga tgcgccggat tcgtgtgagc
      961 agggctaagc acttagcgtg ccagccctat gtcagcgcgc agggcattgt gccacaaggc
     1021 tgcgagcccg aaatcggtgc ctcggcccgg tgcggatgca cttcaatggt gcagatgtgc
     1081 tcccggaatg cactactatg gtgcgagctc cacgaagacg cggatgttgc ggtagtgcgg
     1141 atgttccgcg agccaagtct ggcggcgcga tcgcagtgca tcgagcgcgt ccgcatcggg
     1201 gagggagggc aggatgattt ccacttccac gcgctggccg aggtagtgga tctgcacccg
     1261 ccgagggtcg gcgaagccgg ggccgaggag catgcgcacg tgcgcgagga tttcgtcgcg
     1321 ctcgggcagg gggccgggcg gcaccgcctg cagctcgcca tcgtcctcgg ggtcgatatg
     1381 caccagcacg tcgcgcacct cggggtgggc ggcgcgcacg cgcaggtaca ccgcgtcgga
     1441 cacgcggtgc ccctcggaga cggtcagccg tggatcgacc tggacgtggg cgtcgcacag
     1501 cacgcggtcg gccatccgcc gggtacgcat ttcgtgcagg ccgatcacgc ccggcgtctc
     1561 gcggatcgtc gtgcgcaggc gctcgagctc ctcctccggc aggccggtgt cgatcagttc
     1621 gcgcaccgac ttcaacgcca ggcggatgcc catgtgcagg atcaggaagc cgaccaccgc
     1681 cgcggcgagc ggctccagga aggggtagcc ggccaggctg ccgccgatgc cggcggccac
     1741 gaccagcgag gacgccgcgt ccgagcgtgc atgccaggcg ttggcctcga gcatcggcgc
     1801 gttgaggcgg cgtgcggcgg cgagcgagaa gcggaacagg ccttccttcg cggcgagggt
     1861 cagcagtgcc attcccaggg ccgccggatg cagtaccggc aaggccccga tgttctgcag
     1921 gcggatcccc gaactccaca ggaagccgac gcccaccgcc gccaggacgg agccgagcac
     1981 cagcgtggtg acggtctcga tgcggccgtg tccgtatgga tggttggtgt cggccggatc
     2041 ggcgctgtgg cggccggcga gcagcaccag caggtcggtg atgaggtcgg agagggtgtg
     2101 ggcggcgtcg gccaccaggc tgaacgcgcc ggcgaacagg ccgatgacga cctggccgat
     2161 ggtgagcacc gtgttggtca cgatggccac cagggtggca cgctggatgc cacggctgcg
     2221 ggcgaggtcg tccgcgcccg cagagggacg cgcaaccggg gaggaggctt ggcgggagtg
     2281 catgaggggg gcgcggcgga cgcgctatac ttcgaccgcc ctcattttag acggatgttc
     2341 gcatgggaac cctgtcggaa ctgttgacgc tcgcccacga gcgcgccgaa agccttgggc
     2401 tgccctacca gggcgcgctc acccccgccg aaacctggca ggtgatgcaa ctcgcccccg
     2461 gtgccaagct ggtcgatgtg cgcacgcgcg ccgagctcga ctgggtgggg cgcgtgcccg
     2521 gcgcgatcga gatcgagtgg aagagctatc cctcgatgca ggagaacccc aacttcctcg
     2581 cccagttgaa gcaccaggtc gaccccgagg cgctggtgct gttcctgtgc cgttccgccg
     2641 tgcgctcgga ctccgccgcg cggctcgccg gcgccaacgg ctacgccaac tgctacaacg
     2701 tgctcgaagg cttcgaaggc gacaaggacg ccaacaacca gcgcaaccgc atcggcggct
     2761 ggcgtctcgc cggactgccc tggtaccagg gctgagcggc aacaaatcct tacccggccc
     2821 cgcccgccgc ggtcgaaccc gcggccgcat gcggggtccg acgcccgcaa ccccgggatt
     2881 gccccctccc aacggatccg gataccgaaa caatgcaagt ctccaccatc agcttcgacc
     2941 gcttcaacgt gctcgccgac aacgaggcgc aggagcgcat ccgcgccgcc cgcgcacgcc
     3001 tcggcgagcg ggcggtgctg ctctgccacc actaccagcg cgccgacgtg taccagcacg
     3061 ccgacctcac cggcgactcg ttgaagctcg cacgcctggc ctcgcagacc gacgccgagt
     3121 acatcgtgtt ctgcggcgtg cacttcatgg ccgaggtcgc cgacatcctt tccaagccca
     3181 gccagatcgc catcctgccc gacctcgccg ccggctgctc gatggccgac atggcgagcc
     3241 tggccaaggt cgagcgctgc tggcgcgagc tcgccgaggt gctcggaaag cccgatgcgc
     3301 tgatcacccc ggtcacctac atcaactcgg cggccgacct caaggccttc tgcggcgagc
     3361 acggcggcat cgtgtgcacc tcgaccaacg cgccgaccat cctcgactgg gcgttctcga
     3421 agcgcgagaa ggtgctgttc ttccccgacc agcacctcgg gcgctggacc ggcttcaaga
     3481 agggcatccc gctcgacgag atggtggtgt gggaccccga cctcgagtac ggcggcctca
     3541 cccccgagca gatccgcaag gcgaagatcc tgctgtggaa ggggcactgt tcggtgcacc
     3601 agatgttcca gccggtgcac atcgaccgct ggcgcgagaa gcacccccac ggcttcgtca
     3661 tctcccaccc cgagagcatg ctcgaggtgt gtcagaagtc cgattacgtc ggctccaccg
     3721 agttcatcct caacaccatc acccaggcgc cgcccggcac gcactggctg gtgggcaccg
     3781 agctcaacct ggtgagccgc ctcgccgagg agatgaagcc gcagggcaag gtggtgcagt
     3841 tcatggcgcc cacggtgtgc atgtgctcga ccatgcagcg catcgacccg caacacctgg
     3901 cgtggacgct ggagaacctc gccgagggca aggtggtgaa tcagatcaag gtgcccgcgc
     3961 acgaggccga gctcgcccgc atcgcgctcg accgcatgct cgcggtgtcc tgaacacgcg
     4021 atccgcgctc gcaagaaccg ggggcgtctc ccgcgagggg gggcgccccc gttgcgtcca
     4081 ccgggcggtg aagccgcctc agccgcgcgg ccagaggacc atctgcgtgc agcggaagag
     4141 cgcgatcacc cgtccgctgg cctcgtcggt gacggtcgcg tcccacacct gggtggtgcg
     4201 tccgaggtgc tgggcggtcg cgctgcatgc gatcgcgccc tcgcgcaccg tccccaggtg
     4261 gttgctcttg agctcgatgg tggtgaagga gtccgcgccc gcgggcaggt gggcgatggt
     4321 cgcgtagccg caggtggtgt cggccagcgc gacgacgctc gcggcgtgga ggaactcgtt
     4381 cggtgccgcg tgcagtttct ttaccggcat ccggctgttc agccggcgct cgtcgagcgc
     4441 cagcatctcg atgccgaggt agccgggcag gtactcgccg ccggccttgt tcagggtgtg
     4501 gatgctgact tcgggacgca gtgcgctcat ctggggctcc ttcatcaaga ttcgcaatgg
     4561 aaagcgttgg tgaggatagt cttcttattg cttccggata agccgataat ccggttcgtt
     4621 gcgtcaacgg atcattgaca tgagctggtt accggcctgg gagagcttcg ttcgcgtggt
     4681 cgagggcggc agcatggcgg ccgcggcgcg cacgctcggc tgcacgcgtg cccaggtcag
     4741 caagcaggtc gccgagctcg aacgccgcct cggcgccgcc ctcttcgagc gctccacccg
     4801 gcggctcgtc ctcacccccg ccggggaggt gttcctgcgc cacgcagcgc gcgcactgga
     4861 ggccgtggac agcagcgaga tcgcggtgcg caacctcggc gagcgcccga gcggcacgct
     4921 gcgggtgagc gcgacgctcg ccttcgggcg tcacttcgtc gcgcccctgg tgcccgtcgt
     4981 cacggcggcg aaccccgggc tggagatcga gctggtgctc accgaccgcc tggtcgacct
     5041 ggtggacgac cgcatcgacc tcgcgctgcg catgaccgcg cggccgccgg aggatgcggt
     5101 cgcgcgccac ctgctgcgcc tgcgctgggt gctctgcgcg gcgagcgcct atgtcgagcg
     5161 ccacggcagc ccgcgcgagc cggccgagct ggcgcagcat gcgtgcatcg gccaccccgc
     5221 gatcggcgcc gccccctggg gattggtcgc cgcggccggc gcgcaggccc gcgtggcggt
     5281 gcgcagccgc gtccgtttcg acagcctcga ttgcacgctc gacgcggtcc tcgccggcca
     5341 cggcatcgcc atcctgcccg cctacctgtg cgagcccgcg ctgcaggccg gcagcctgcg
     5401 caccgtgctc gacggctggg agcccgcgtt cgacgtcggc cgcgacctgt acgcctgcta
     5461 caccccgggc agggtggggg tatccaaggt gcgcgtcttc atggatgcgt tgcagcagcg
     5521 gctgcagccg ctgccgtcgt gggcctgcgc cgcgggtgtg ggcggatgag aaaggcggtg
     5581 aaagccccgg cgaaaggtgg cgagccgggg cgggggcacg gccgacgaat ccgtgccgtc
     5641 gatcgtgcgc cggaggcttt aacatccgct tcccgcccgc atatgatgtc gcgtggcctt
     5701 caccccggcc ggagtcgcct ccgctcatgc ccctgcgtat ctgctcctac aacatccaca
     5761 agggtttctc gcagttcaac cgccgcatgg tggtgcatga gctgcgcgac cgcctgcgca
     5821 gcctggatgc cgacctcgtc tttctgcagg aggtgcaggg cctgcacctg ggccatccgg
     5881 cacgccaccc cgactggccc gcgctgccgc agcacgagtt cctcgccgag gacgcctggc
     5941 agcagatcgc ctatggcggc aacgcgatct acgaccatgg ccaccacggc aacgcgatcc
     6001 tgagtcgcca cctcatcctc agcaccgcca accaggacgt ctccgaccat cgcttcgagc
     6061 gccgcggcct gcttcactgt gaggtgcagc tgcccgagtt cgacgtgccg gtgcattgcg
     6121 tgtgcgtgca tctgggcctg atggcgggca gccggcggcg ccagatggag gcgctcgccg
     6181 agcgcatgga agagctcgcc ccgggcaacg cgccgctgat catcgccggc gacttcaacg
     6241 actggcgcaa ccgcgccgac gacctgctcg ggcgccggct cggcctcgcc gaggccttcg
     6301 gcagcggacg cgggcgaccg gcgcgcagct acccgagcac gctgcccttc ttccgcctcg
     6361 accgcatcta cgtgcgcggc ttccgcgtgc gccaggcgca ggtgcattac ggcgcgccgt
     6421 gggcgcagat ctccgaccac gcggcgctga ccgccgacct ggagcccatc ggctgatgca
     6481 gttcctcgcg ggcaacgcgg tcgcgttgct ggagaacggc gagcagtact tcccggcgct
     6541 ggaagccgag atcgaccgtg ccgagcgcga gatcttcctg cagagctaca tcttcgagga
     6601 cgacggtacc ggcgcgcgca tcgccgctgc actcgcacgc gcggcgggcc gcggggtcgc
     6661 ggtgcgggtg atggtggacg gcttcggcgg gcgcagcttc gtctcgcggc tgatgccgcg
     6721 cctgcgcgag gccggcgtgc aggtgctgat ctaccgccgc gagctgcgta ccctgtcgct
     6781 gcgccgccat cgcctgcgcc ggctgcaccg caaggtggtg gcgatcgacg gccgcgtggc
     6841 cttcgtcggt ggcatcaacg tggtggacga cttcgatgcg ggcgcgctgc cgcacccgcg
     6901 ctacgactac gcggtgcgcg tcgaggggcc ggtgctcgcg ccggtggtgg cctcggcgca
     6961 gcgcctgtgg cggctggtgg cgggggcgag ccttcgcagg cgcgccgccc agccgctgct
     7021 ggcgccgccg ctgaccgacg cggcgggctc gatcc
//
LOCUS       DAOQNN010000148         6970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5861_length_6970_cov_11.167607, whole genome shotgun sequence.
ACCESSION   DAOQNN010000148 DAOQNN010000000
VERSION     DAOQNN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5861_length_6970_cov_11.167607"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1871)
                     /locus_tag="PKV97_09235"
     CDS             complement(<1..1871)
                     /locus_tag="PKV97_09235"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HNB06096.1"
                     /translation="MSKQLIIAEKPSVAQDIARALGGFTKEKDYFESDEYVLSSAVGH
                     LLELTLPEEFEVKRGKWTFAHLPVIPPHFAVKPIEKTEDRLKLLTRLIKRKDVTGLIN
                     ACDAGREGELIFNFIAQHAGTKKPMQRLWLQSMTAQAIRDGFAHLRAAQDVEGLRNAA
                     ICRAESDWLIGINGTRAMTAFNSKTGGFHLTTVGRVQTPTLAMVVEREDRIRKFKSRD
                     YWELEARFGCAAGEYPGRWFDEKFKRPEGDEHATAFRLWDKAQAEAIRAKCAGKPGVV
                     SEEAKPSTQLSPLLFDLTSLQREANGRFGFSARVTLQLAQALYEKHKVLTYPRTDARA
                     LPEDYLATVGEVMRTLPDQYAPFANEITRQGWVRPNKRIFNNAKISDHFAIIPTGALP
                     KSLSDAEHKIYDLVTKRFLAVFYPAAEYQITTRITRVEGEAFKTEGKVLVNPGWLTVY
                     GKEAANDEKDAKDGGAPQLVAVKQGETVSTEDIVVKSLQTKPPARFNEATLLSAMEGA
                     GKMVDDEELRAAMAERGLGTPATRAQIIEGLISEQYIHRDGRELIPSAKAFSLITLLK
                     GLGVTALTSPELTGGWEYKLAQMEHGKLSREAFMNEIAEMTREVVERAKRYESDTVPG
                     "
     gene            complement(1975..2445)
                     /locus_tag="PKV97_09240"
     CDS             complement(1975..2445)
                     /locus_tag="PKV97_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HNB06097.1"
                     /translation="MFDILVYLFESYIHADACPEGELLNRKLSAAGFEQDDISEALEW
                     LAGLRRVARDIHPGTAPQAGSVRIYTEEEQALLDGRCRGFLSLLESAGVLGTAHRELI
                     IERAMALADFTITLNRLKVIVLMVLWQQEEPIDTLMVDELLTEEDDWAYEPTVH"
     gene            complement(2529..3692)
                     /gene="dprA"
                     /locus_tag="PKV97_09245"
     CDS             complement(2529..3692)
                     /gene="dprA"
                     /locus_tag="PKV97_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNB06098.1"
                     /translation="MTAPADDLADWLRLACIPGVGTQGQRALLAAFGLPGHIFAAGRG
                     ALAAVVGGQAADAVLAVPAHEDIERTLAWASEAGNRVLTLADADYPRQLFDITDPPVL
                     LYVKGEPALLSRPGIALVGARSATAAGEANAEAFARTLAQQGLVVVSGLALGIDAAAH
                     RGALAAGSTGAGTVAVIGTGIDRIYPARNAALAREIAAAGAVVSEFPLGTPPLQHNFP
                     RRNRLIAGLAEGVLVVEAALGSGSLITARLATETGREVFAIPGSIHSPLSRGCHRLIR
                     DGAKLVETAEDVVEELRGRLGWPAPVAASVKDRRSRGAVPAVPAAPPRQAALALDGER
                     TRVLEAIGHDPVDLDTIAARCGLTVDALYAILLPLELEGRLAKLPGGRFQRIT"
     gene            complement(3724..4764)
                     /locus_tag="PKV97_09250"
     CDS             complement(3724..4764)
                     /locus_tag="PKV97_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNB06099.1"
                     /translation="MMRIIFPLLVAFASLLGPAAHAQSNVRLAPDAPDSYTVVRGDTL
                     WGISGRFLQEPWRWPEVWRLNRDEIRNPHLIYPGQVVLLDRSGPWLSVGRRVGDGGTR
                     DGRLQPKVHSEPIAEALPSIPLQIIEPFLNRPLVVDQPRLDGSATIVATETSRVLTGT
                     GDTVFAKNIGDDADIWHIYRPARALEDPQTREAIAWEAAYLGTARVTERGEPTTLEIV
                     SAVEEIGTGDLMMPSEPPSVFSYAPHAPDFEVEGRILSIYRGVSETGRLNVVALNVGE
                     QDGLERGHVLALHRNRGVAEYRGEDGKESFRLPEKRYGVAFVFRVFERVSYALVMESD
                     GPVTIGDTLRKP"
     gene            4957..5460
                     /gene="def"
                     /locus_tag="PKV97_09255"
     CDS             4957..5460
                     /gene="def"
                     /locus_tag="PKV97_09255"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNB06100.1"
                     /translation="MALLPILRYPDPRLHTVAAPVGRVDDEIRRLIADMAETMYEAPG
                     IGLAATQVDVHKRIVVIDVSEDKSGLMALINPEILERSGEQVCEEGCLSVPGIYEKVS
                     RAERVKVRALNEKGESFEFEADGLLAVCVQHEIDHLDGKVFVEYLSQLKLGRIKSKLA
                     KKARITA"
     gene            5460..6422
                     /gene="fmt"
                     /locus_tag="PKV97_09260"
     CDS             5460..6422
                     /gene="fmt"
                     /locus_tag="PKV97_09260"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNB06101.1"
                     /translation="MAATASGAGGLRVAFAGTPEFAAQALDAILKAAYAVPLVLTQPD
                     RPAGRGMKLSPSAVKQLALAHGIEVDQPEKLRTEEQRARLVACAPDVLVVAAYGLILP
                     PAVLALPRLGCINIHASLLPRWRGAAPIHRAIEAGDAETGITIMQMDEGLDTGPMLLR
                     RALPIAADDTTASLHDRLAVLGGACIVEALAALAAGALVATPQPAEGVTYAAKIGRAE
                     ADIDWSRPAADIERAMRAFDPFPGAVSTLRDTAVKCWKARVVAGEGEPGRVLAVDADG
                     IVVACGRDALRCTVLQRPGSKRLPAGEFLRGFPVSVGERFASAA"
     gene            6513..>6970
                     /locus_tag="PKV97_09265"
     CDS             6513..>6970
                     /locus_tag="PKV97_09265"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNB06102.1"
                     /translation="MFGNWIKTSILMAGIVALFGAMGAAIGGQQGMLIALVMGGAMNV
                     WAYWFSDKMVLKMYKAREVDAASSPYLYNMVAELARRAELPMPKVYIIDEAQPNAFAT
                     GRNPDNAAVAATTGIIRMLSERELRGVMAHELAHVKNRDILISTISATIAG"
BASE COUNT         1014 a         2319 c         2513 g         1124 t
ORIGIN      
        1 ccgggcacgg tgtcggactc gtagcgcttg gcgcgctcga ccacctcgcg cgtcatctcg
       61 gcgatctcgt tcatgaaggc ttcgcggctg agcttgccgt gctccatctg cgcgagcttg
      121 tattcccagc cgccggtgag ctcgggcgag gtcagcgcgg tgacgccgag gcccttcagc
      181 agcgtgatca gcgagaaggc cttggcgctc gggatcagct cgcggccgtc gcggtggatg
      241 tactgctcgc tgatcaggcc ctcgatgatc tgcgcgcgcg tggccggggt gccgaggcca
      301 cgttcggcca tcgccgcgcg cagctcctcg tcgtcgacca tcttgcccgc gccttccatg
      361 gcggagagca gcgtggcttc gttgaagcgc gccggcggct tggtctgcag cgacttgacc
      421 acgatgtcct cggtggacac cgtctcgccc tgcttcaccg ccaccagctg cggggcgccg
      481 ccgtccttgg cgtccttctc gtcgttggcg gcttccttgc catacacggt cagccagccc
      541 ggattgacca gcaccttgcc ctcggtcttg aaggcctcgc cctcgacgcg ggtgatgcgg
      601 gtggtgatct ggtactcggc cgcggggtag aacaccgcca ggaagcgctt ggtgaccagg
      661 tcgtagatct tgtgctcggc gtccgacagg ctcttcggca gcgccccggt ggggatgatg
      721 gcgaagtggt cggagatctt tgcgttgttg aagatgcgct tgttcggccg tacccagccc
      781 tgcctggtga tttcgttggc gaagggcgcg tactggtcgg gcagcgtgcg catgacctcg
      841 cccacggtgg cgagatagtc ctcgggcagc gcgcgcgcgt cggtacgcgg gtaggtcagc
      901 accttgtgct tttcgtacag cgcctgcgcc agttgcaggg tcacgcgggc ggagaagccg
      961 aagcggccgt tggcctcgcg ctgcaggctg gtgaggtcga agagcagcgg cgacagctgg
     1021 gtggagggct tggcctcctc gctgaccacg cccggtttgc ccgcgcactt ggcgcggatg
     1081 gcctcggcct gggccttgtc ccacaggcgg aaggcggtgg cgtgctcgtc gccttccggg
     1141 cgcttgaact tctcgtcgaa ccagcgcccg gggtattcgc cggcggcaca gccgaagcgc
     1201 gcctcgagtt cccaatagtc gcgcgatttg aacttgcgga tgcggtcctc gcgctcgacc
     1261 accatcgcca gcgtcggcgt ctgcacgcgg cccacggtgg tgaggtggaa gccgccggtc
     1321 ttggagttga acgcggtcat cgcgcgcgtg ccgttgatgc cgatcagcca gtcggattcg
     1381 gcgcggcaga tcgcggcgtt gcgcaggcct tcgacgtcct gcgcggcgcg caggtgggcg
     1441 aagccgtcgc gaatcgcctg cgccgtcatc gactgcagcc acaggcgctg catcggcttc
     1501 ttcgtgccgg cgtgctgggc gatgaagttg aagatcagct cgccttcgcg gcccgcgtcg
     1561 caggcgttga tgaggccggt gacgtccttg cgcttgatga gtcgggtgag cagcttgagg
     1621 cggtcctcgg tcttctcgat cggcttgacc gcgaagtggg gcgggatcac cggcagatgg
     1681 gcgaaggtcc atttgccgcg cttgacctcg aattcctcag gcagggtgag ctcgagcagg
     1741 tggccgacgg ccgacgacaa tacatattcg tcggactcga agtagtcctt ctccttggtg
     1801 aagccgccca gcgcacgggc gatgtcctgc gcgacggaag gcttttccgc gatgatcagt
     1861 tgcttgctca tgctcgacgg tcaacgaggg tcgcgccgcg agggcgcgtg ggttcggaag
     1921 cggcggatga taaggacggc ccgccggaac aggcaagccg agcgggtgga ccggtcagtg
     1981 caccgtgggt tcgtaggccc agtcgtcttc ctcggtcagc aactcgtcca ccatcagggt
     2041 gtcgatcggc tcttcctgct gccacagcac catcagcacg atgaccttga gccggttgag
     2101 cgtgatggtg aagtcggcca gcgccatcgc gcgttcgatg atcagctcgc ggtgggcggt
     2161 gccgagcacg ccggcgctct cgagcaggct caggaagccg cggcagcggc cgtcgagcag
     2221 ggcctgctct tcctcggtat agatgcgcac cgacccggcc tgcggtgcgg tcccggggtg
     2281 gatgtcgcgg gcgacgcggc gcaggccggc gagccactcc agcgcttcgg agatgtcgtc
     2341 ctgctcgaag cccgccgcgg acagcttgcg gttgagcagc tccccttccg ggcaggcgtc
     2401 ggcgtggatg tagctctcga agaggtatac gaggatgtcg aacaaggcgg cctctcgtgg
     2461 atcggcaggt gagacggaag ggccgggccc ccgctggggc atggggacgg cggctcgcgg
     2521 ccgctctttc aggtgatgcg ctggaagcgt ccgccgggca gcttggcaag gcgcccttcc
     2581 agttcgagtg gcagcaggat ggcgtagagg gcatcgaccg tcaagccgca gcgtgcggcg
     2641 atcgtgtcga ggtccaccgg atcgtggccg atcgcctcga gcacgcgtgt gcgctcgcca
     2701 tcgagcgcga gcgcggcctg gcgagggggt gcggccggga cggcgggtac cgcgccgcga
     2761 ctccggcggt cctttaccga tgcggcgacc ggcgctggcc agccgaggcg gccgcgcagt
     2821 tcctcgacga cgtcctccgc cgtctcgacc agcttcgcgc cgtcgcggat caggcgatgg
     2881 cagccgcgcg acagcggcga gtggatcgag ccggggatcg cgaacacctc gcggccggtc
     2941 tcggtggcga ggcgcgcagt gatcagcgag ccgctgccga gcgccgcctc gaccaccagt
     3001 acgccctcgg ccagtccggc gatcaggcgg ttgcggcgcg ggaagttgtg ctgcagcggc
     3061 ggcgtgccga ggggaaactc gctgaccacc gcgccggcgg cggcgatctc gcgcgcgagt
     3121 gcggcgttgc gcgccgggta gatgcggtcg atgccggtgc cgatcaccgc caccgttccg
     3181 gcccctgtgc tgccggcggc gagcgcgccg cggtgtgccg cggcgtcgat gccgagcgcc
     3241 agtccgctga cgacgacgag gccttgctgg gcgagcgtgc gcgcgaaggc ctcggcgttg
     3301 gcttcgccgg ccgcggtcgc cgagcgtgcg ccgaccagcg cgatgcccgg gcgcgatagc
     3361 agcgcgggct cgcccttgac gtagagcagc accgggggat cggtgatgtc gaagagttgg
     3421 cgcgggtagt cggcgtcggc cagggtcagg acgcggttgc cggcctcgct cgcccatgcg
     3481 agcgtgcgct cgatgtcttc gtgtgccggc acggcgagca cggcgtccgc cgcctgaccg
     3541 cccaccaccg cggcgagcgc gccgcgtccg gcggcgaaga tgtggccggg caggccgaag
     3601 gcggcgagca gggcgcgctg gccctgcgtg ccgacgcccg ggatgcaggc gaggcgcagc
     3661 cagtcggcga ggtcgtcggc aggcgcggtc acgatcgggc ggctgcggct tgccggtgcc
     3721 ggatcagggc ttgcggaggg tgtcgccgat ggtgaccgga ccgtcggact ccatcaccag
     3781 cgcgtacgag acgcgctcga agacgcgaaa cacgaaggcc acgccgtagc gcttttccgg
     3841 caggcggaag ctttccttgc cgtcctcgcc acggtactcg gcgacaccgc ggttgcgatg
     3901 gagtgcgagc acgtggccgc gttcgaggcc gtcctgctcg ccgacgttga gcgcgaccac
     3961 gttgagacgg ccggtctcgc tgaccccacg atagatggag aggatcctgc cctcgacctc
     4021 gaagtccggt gcatgcgggg cgtaggaaaa cacgctcggc ggctcgctcg gcatcatcag
     4081 gtcgccggtg ccgatctcct cgaccgcgga gacgatctcc agcgtcgtcg gttcgccgcg
     4141 ctcggtgacg cgggcggtgc cgagataggc ggcttcccag gcgatcgcct cgcgcgtctg
     4201 cgggtcttcg agcgcgcgcg cggggcgata gatgtgccag atgtccgcgt cgtcgccgat
     4261 attcttggcg aagacggtgt cgccggtgcc ggtcagcacg cggctggtct cggtggcgac
     4321 gatggtggcc gagccgtcga ggcggggctg gtcgaccacc agcggacggt tcaggaaggg
     4381 ctcgatgatc tgcagcggaa tgctcggcag ggcctcggcg atcggctcgc tatggacctt
     4441 cggctgcagg cggccgtcgc gggtgccgcc atcgccgacg cgccggccca cgctgagcca
     4501 tggcccgctg cggtcgagca ggacgacctg gccaggatag atgagatgcg ggttgcggat
     4561 ttcgtcgcgg ttcaggcgcc agacctcggg ccagcgccaa ggctcctgca ggaagcgccc
     4621 ggagatgccc cacaaggtgt cgccgcgcac cacggtgtag ctgtcggggg cgtcgggggc
     4681 gaggcgcacg ttcgactgcg cgtgcgcggc agggcccagc aggctcgcga aggcgacgag
     4741 cagagggaat ataattcgca tcattggacg catccgatcg ggacctcgcg tgccgggttc
     4801 gacccggaac gctggctatg cgccggggtc gaagcaacga accgcgcagt gcgcgcgtgc
     4861 tgcataccgg tcgcggatat tccggctaaa tacatgccga agcgtgtgaa aacgcttgaa
     4921 attctgcact caaaggctta acccagcaag gtattcatgg ctctcctacc cattctccgc
     4981 taccccgatc cccgcctcca cacggtcgcc gcgccggtcg gccgggtcga cgacgaaatc
     5041 cgcaggctga tcgccgacat ggccgagacc atgtacgaag cgcccggcat cgggctggcc
     5101 gccacccagg tcgatgtgca caagcgcatc gtcgtcatcg acgtgtccga ggacaagtcc
     5161 ggcctgatgg cgctgatcaa ccccgagatc ctggagcgtt cgggcgaaca ggtgtgcgag
     5221 gaaggttgcc tgtccgtacc cggaatctac gagaaggtgt cgcgcgccga gcgtgtgaaa
     5281 gttcgcgccc tgaacgaaaa gggtgaatcc ttcgagttcg aggccgacgg cctgctcgcg
     5341 gtatgcgtgc agcacgagat cgaccatctc gacggcaagg tcttcgtcga atacctgtcg
     5401 cagctcaagc tcggccgcat caagtccaag ctggccaaga aggcccgcat caccgcgtga
     5461 tggccgccac cgcatccggg gccggcggcc tgcgcgtcgc cttcgccggc acccccgaat
     5521 tcgccgcgca ggcgctcgac gccatcctga aggccgctta cgcggtcccg ctcgtgttga
     5581 cccagcccga ccgccccgcc ggtcgcggca tgaagctcag cccgagcgcg gtcaagcagc
     5641 tcgcgctcgc ccacggcatc gaggtcgacc agcccgagaa gctgcgcacc gaagagcagc
     5701 gtgcccgcct cgtcgcctgc gcgcccgacg tgctggtggt cgccgcctac ggcctcatcc
     5761 tgccgcccgc ggtgctggcg ctgccgcgcc tgggctgcat caacatccac gcctcactgc
     5821 tgccgcgctg gcgtggtgcg gcgcccatcc atcgtgcgat cgaggccggc gacgccgaga
     5881 ccggcatcac catcatgcag atggacgagg gcctggacac cggcccgatg ctgctgcggc
     5941 gcgcgctgcc gatcgccgcg gacgacacca ccgccagcct gcacgaccgc ctcgccgtac
     6001 tcggcggcgc atgcatcgtc gaggcgctcg ccgcgctcgc cgccggcgcg ctggtcgcga
     6061 cgccgcagcc ggcggagggc gtcacctacg ccgccaagat cggccgcgcc gaggccgaca
     6121 tcgactggag ccggccggcg gcggacatcg agcgcgccat gcgtgccttc gatcccttcc
     6181 cgggggccgt gtccaccctg cgcgacaccg cggtcaagtg ctggaaggca cgggtcgtgg
     6241 ccggcgaggg cgagccgggc cgggtgctcg cggtcgatgc ggacggcatc gtcgtggcct
     6301 gcgggcgcga cgcgctgcgt tgcacggtgt tacagcgccc gggcagcaag cgcctgcccg
     6361 cgggggaatt cctgcgcggt ttcccggtct ctgtgggcga acgcttcgcg tctgcggcgt
     6421 gagcgcgcgg cgcgtccgtg gcggccttga aggcggtcgc ggacgcccca agctacactg
     6481 acgaatcaat ctctcgcagg aaggatcccg caatgttcgg aaactggatc aagacctcca
     6541 tcctgatggc cggcatcgtc gccttgttcg gggcgatggg cgcggccatc ggcggccagc
     6601 agggcatgct gatcgcgctc gtgatgggcg gcgcgatgaa cgtctgggcc tactggttct
     6661 cggacaagat ggtgctgaag atgtacaagg cgcgcgaggt cgatgccgcc tcctcgccct
     6721 atctgtacaa catggtggcg gagctcgccc gccgtgccga gctgccgatg ccgaaggtgt
     6781 acatcatcga cgaggcccag cccaatgcct tcgccaccgg ccgcaacccc gacaacgccg
     6841 cggtggcggc gaccaccggc atcatccgca tgctctccga gcgcgagctg cgcggggtga
     6901 tggcgcacga actggcacac gtgaagaacc gcgacatcct gatctcgacc atctcggcca
     6961 ccatcgcggg
//
LOCUS       DAOQNN010000149         6953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5885_length_6953_cov_13.223978, whole genome shotgun sequence.
ACCESSION   DAOQNN010000149 DAOQNN010000000
VERSION     DAOQNN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5885_length_6953_cov_13.223978"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKV97_09270"
     CDS             <1..703
                     /locus_tag="PKV97_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNB06103.1"
                     /translation="GSATAGEDGVIRVMWWDGDSSKYRVKNGIIGESVDAAGNVLKPN
                     RYYELNEEHKFISLSLDKRIEVVRGDITAQEEPFDLIVNAANESLLGGGGVDGAIHKA
                     AGPALLDACKSLNGCLPGDAKITKGFKLRARFIAHAVGPRWVDGKAGEAEVLAAVYKR
                     VIELATEKGLTSIAAPSISTGAFGYPLGEASGIAMRTVAALLENARIRRFRFVCFDAK
                     TERAYDAAKRTVLGL"
     gene            754..1050
                     /locus_tag="PKV97_09275"
     CDS             754..1050
                     /locus_tag="PKV97_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06104.1"
                     /translation="MPKKTKDLRHQLTMALFDLPRMTISQQDRELLNEVRARLEVLEQ
                     IETRAAEVKTKARKVYEDTSIDAVSCLDDCADYILGKRERFALHEFHDSAKKRG"
     gene            1057..1836
                     /locus_tag="PKV97_09280"
     CDS             1057..1836
                     /locus_tag="PKV97_09280"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNB06105.1"
                     /translation="MAKHTKKISPQLVGILSRLQMARGPMAWVARITDGQLDRKVYVL
                     LNEALEALGGKWHKGVKGHIFAEDPTEAIEELILTGEYKTKFAGDFFQTPISIAQRMA
                     EWAVRSGDRVLEPSAGAGRLVRAAFSAGASAVVCVELDEKRATGLRGFLPDADVTVVC
                     ADFLTVGPEQIGEDFDAIVMNPPFMRGAAARHILHAMRFLKRGGRLATLAPLSLRFRE
                     DAAHKELREIISEEDVETLEPKSFAAEGTNIDILLATWQKP"
     gene            1833..3680
                     /locus_tag="PKV97_09285"
     CDS             1833..3680
                     /locus_tag="PKV97_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNB06106.1"
                     /translation="MKSTITHFHLFCGVGGGALGFNRGEARFGKLVADFRCVGGIDSD
                     ASACRDFTRLVGTPATCLDLFSREQYERFYGHRPPPDWCEATVDDVRRAAGGQRPDIF
                     FTSPPCKGFSGLLSSEKSRSPKYEALNELVLRGLLLALEAWSDEPPPLILLENVPRIQ
                     QRGRSLLERVQGLLRGYGYASRETVHDAGELGGLAQHRRRFLLVARHEQQVPSFLYEP
                     SRRRVRPIGDVLSELPPPCGSPLNPLHRLPNLTAETWMRLALIPAGKDWRALRNMDLV
                     KLALVRYGSHRGKLRVEDWAQPAHTVTGSDRVGSGALSVADARWNRGQQYGVLPMDQP
                     SGAVTAQAAPGSGRFSVADPAAPREWFRNILRVTDYQQPSGTVTGACRPSAGAISVAD
                     PGWHKGVLGVLPLDQPIGTVPGRSSPTNGAFSIADTLKRGPRFNNVYRLVEWRDTSPC
                     VTAGGSPTSGGLNVADPRAAWQNAGHYGVVSWGETAGAVTAAASHDNGRNNVADPRPL
                     RLEELGEAPLIVSLDGTWHRPLTTLELAALQGFPTEGLTFDGRAMQRHREKIGNAVPP
                     PAAQAIAGVMGQVLLMARSGQTFALSSTPVWVRPVALALTVDGEAVWTR"
     gene            3668..4402
                     /locus_tag="PKV97_09290"
     CDS             3668..4402
                     /locus_tag="PKV97_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06107.1"
                     /translation="MDPLKPFPPTVEYIRDGEDMPQAFRIACEQPAWSQLLRWRRRGP
                     NLYPHTPHHAGLPDPEPLDTIVTMAMRLPQVEMRIVLGCEDLETIAAELLLRLRPIPE
                     IMRGSIGIIVEAYRHDIGLTPWRPGRRGIRGEGVRDIARLAAGDAALTEKRAAVYRWL
                     MGLWMPPERWEPEVFQGFRHHEVTFVDGPLWAAWELSPIGVTATGEYGVHPGARAWYR
                     IGRCRFKSGDSPARMVDEVLQRTKRL"
     gene            4428..5078
                     /locus_tag="PKV97_09295"
     CDS             4428..5078
                     /locus_tag="PKV97_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06108.1"
                     /translation="MTKLHYMNSIASSESRKSAALLTSLFEDLCTPTVTVSVGEVGIV
                     VSEEGFCTSGAMGTAEASWSPVVIEGGKGKSETVAEHIVSAAKQYDYKSVGQKYDPKL
                     SVTEIAARIRAEIKAAQKGGELPRSMKVSVRKRSCTHSWAIDVSATNIGCELYTDDYK
                     RAQAEHRDFPWHQRFTAEADRILSKLQAIWSAYNFDDSDSMTDYFHVNYYGGVKID"
     gene            5086..5544
                     /locus_tag="PKV97_09300"
     CDS             5086..5544
                     /locus_tag="PKV97_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06109.1"
                     /translation="MTPIKLTKYTIQPLPSLGLPLEVHRYSSGLVLAVHDDQPDVRFA
                     SLEALCDEHHLDLSKYKRLEDVFELSVSVLEFGTYAKGVYAPAGALHGFDPEHPEVDY
                     NTERADRLYLALGIAPDTGEQLGRLPDGRWALVTTEVPGNRFAIEDVATT"
     gene            5541..5753
                     /locus_tag="PKV97_09305"
     CDS             5541..5753
                     /locus_tag="PKV97_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB06110.1"
                     /translation="MTAGERIRARRTELKLTQAQLTQALGLKNRQSVNRMELGHQAVS
                     AEQAAKLAPLLKVSPAWLLFGAADES"
     gene            5743..6030
                     /locus_tag="PKV97_09310"
     CDS             5743..6030
                     /locus_tag="PKV97_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06111.1"
                     /translation="MKVDITDHAIDRYRERIEPGLSYEEARERIAEGMERAIRLREKT
                     YAGSDMWHLAGTCVFVVERCRHVHKLHPVKTVITEAEAFGRDEEETPDGND"
     gene            6017..6517
                     /locus_tag="PKV97_09315"
     CDS             6017..6517
                     /locus_tag="PKV97_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06112.1"
                     /translation="MATIKVSGGKFSCPSEITLIAGGEKFDLRKIQLGMDEPLLPPLE
                     FHTMLEFCDREITKAVLGPLLQVEAPAVAPSMMRALVHAHYTEAKMKEDWVRFAALYA
                     IPSCDPQKPKRPKWGQRGRHRLRKTRLEARAINHLINVIRGELLCQKPKATLFTMETS
                     HPPTVV"
     gene            6454..6753
                     /locus_tag="PKV97_09320"
     CDS             6454..6753
                     /locus_tag="PKV97_09320"
                     /inference="COORDINATES: protein motif:HMM:NF022150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HNB06113.1"
                     /translation="MSKTQGHPVHDGNKPPADGGVTKKAHRGFAAMKPEKQKAIASMG
                     GKAAHERGYAHEFTEEEARLAGQKGGRAISADRDHMASIGRRGGLARHQKAQQPT"
BASE COUNT         1303 a         2248 c         2256 g         1146 t
ORIGIN      
        1 aggcagcgcc accgcgggcg aggatggggt catccgtgtg atgtggtggg atggggacag
       61 cagcaagtac cgcgtgaaga acggaatcat cggcgagagc gtagatgccg ccgggaatgt
      121 gctcaagccg aatcgctact acgagctgaa cgaagagcac aagttcatct cgctgtctct
      181 cgacaagcgc atcgaagtgg tgcgcggcga catcaccgcc caggaagaac cgttcgacct
      241 catcgtcaac gcggcgaacg agtcgctgct cggcgggggc ggcgtcgatg gcgccatcca
      301 caaagctgcg gggcctgccc tcctcgacgc ttgtaagtct ctgaacggct gcctgccagg
      361 tgacgcgaag atcaccaagg gattcaagct gcgcgcccgc ttcatcgcgc acgcggtggg
      421 gccgcggtgg gtcgatggca aggccggcga agccgaggtc ttggcggcgg tctataagcg
      481 cgtcatcgag ttggccaccg agaaagggct cacctcgatc gcagctccgt cgatttcgac
      541 tggtgctttc ggctacccgc tgggcgaggc cagcgggatc gcgatgcgca cggtcgctgc
      601 gctgctcgaa aatgctcgga tccgtcgctt ccgcttcgtc tgcttcgacg cgaagaccga
      661 gcgggcctac gacgcggcca agcgcaccgt gctcggcctg taggaccagc cagcgccagc
      721 agttcggggg ggactgctag cggagaggaa gccatgccga agaagacgaa agatcttcgc
      781 catcagttga cgatggcgct cttcgatctg ccacggatga ccatcagtca gcaggatcgc
      841 gaactgctca acgaggttcg cgcgcgactg gaagtcctcg agcaaatcga gacccgagcg
      901 gctgaggtga agaccaaggc acgcaaggtc tacgaagaca ccagcatcga cgcggtgagc
      961 tgtctcgacg actgcgccga ctacatcctt ggcaagcgcg agcgctttgc cctgcacgag
     1021 ttccacgaca gcgcgaaaaa gcgcggatag ggatttatgg ctaagcacac gaaaaagatc
     1081 agccctcagc ttgttggcat cctcagtcgc ctgcaaatgg ctcgaggtcc gatggcatgg
     1141 gttgcccgta ttaccgacgg tcagcttgac cgcaaggtct acgtgcttct gaacgaggcg
     1201 ctcgaagccc tcggcggcaa gtggcacaag ggcgtgaagg gacacatctt cgccgaggat
     1261 ccaaccgagg cgatcgaaga actgatcctc accggcgagt acaagaccaa gttcgcgggt
     1321 gactttttcc agacaccgat cagcatcgct cagcgcatgg ctgagtgggc agtgcgttcc
     1381 ggcgaccgcg ttctcgagcc gagtgctggc gctggtcgcc tcgtgcgagc tgctttctcc
     1441 gcgggcgctt ccgcggtcgt gtgtgtcgag ctggacgaaa aacgcgccac gggcttgcgg
     1501 ggtttcctgc cagatgccga tgtcaccgtg gtctgcgcgg atttcctcac ggtcggcccc
     1561 gagcagattg gcgaggactt cgacgcgatc gtgatgaacc cgcccttcat gcgaggcgcg
     1621 gcggcgcggc acatcctgca tgcgatgcgc tttctcaagc gcggcgggcg tctcgcgacc
     1681 ttggcccccc tcagcctgag attccgcgaa gacgcggccc ataaggagct gcgcgagatc
     1741 atcagcgaag aagatgtcga gacgctcgag ccgaagtcgt ttgcagccga gggcaccaac
     1801 atcgacatat tgctcgcgac ctggcagaag ccatgaagtc gacgatcacc cactttcacc
     1861 tcttctgcgg agtcggcggc ggcgcgctcg gtttcaaccg gggcgaggcc cgcttcggaa
     1921 agctcgtcgc cgacttccgc tgtgtcggtg gaatcgactc ggacgccagc gcctgccgcg
     1981 acttcacacg cctcgtgggc acgccggcca cctgcctgga cctcttcagc cgcgagcagt
     2041 acgagcgctt ctacgggcac cggccaccgc cggactggtg cgaagccacg gtcgatgatg
     2101 tgcggcgcgc agcgggtggg cagcgtcccg acatcttctt cacgtcgccg ccgtgcaagg
     2161 gcttcagcgg cctgctcagc tccgaaaaga gccgctcccc gaagtacgag gcgctgaacg
     2221 agcttgtgct gcgcggcctg ctgctcgcgc tcgaagcctg gtcggacgag ccgccgccgc
     2281 tgatcctcct cgagaacgtg ccgcgcattc agcaacgcgg tcggtcgctt ctcgagcgtg
     2341 tgcagggcct gcttagaggc tacggatacg cctcgcgcga gaccgtccac gatgccggcg
     2401 aactcggagg gctggcgcag caccgccgtc gtttcttgct cgtcgcgcgg catgagcagc
     2461 aggtgccgag cttcctctat gagccgagcc gccgccgcgt ccgcccgatc ggcgatgtgc
     2521 tgagcgagct gccgccgccg tgcgggtcgc cgctgaaccc actgcaccgg ctgccgaacc
     2581 ttaccgccga gacctggatg cgcttggctt tgatcccggc cggcaaagac tggcgggcgc
     2641 tgcggaacat ggacctcgtc aagctggcgc tggtgaggta cggctcgcac cgaggcaaac
     2701 tgcgggtcga agactgggcg cagccggccc ataccgtcac tggaagcgat cgggtaggca
     2761 gcggggctct atccgtcgca gatgctcggt ggaaccgcgg ccagcaatac ggcgtcctgc
     2821 cgatggacca gccctcgggc gcggtgacag cgcaggcggc gcctggcagc ggccggttct
     2881 cggtggccga tcctgctgcg ccgcgggagt ggttccgcaa catcctgcgc gtcaccgatt
     2941 atcagcagcc gtcagggact gtcaccggag cttgccggcc ctcggcgggt gcgatctctg
     3001 tcgctgaccc cggctggcac aagggcgtgc tcggtgtgct gccgctcgac cagccaatcg
     3061 gcacggtccc cggccgctca tcgccgacga acggggcgtt ttcgatcgcg gacaccctca
     3121 agcgcgggcc gcggttcaac aacgtgtatc gcctcgtcga gtggcgggac accagtccct
     3181 gcgtcaccgc tggcggatcg ccgaccagcg gtgggctcaa cgtcgcggac ccgcgcgcgg
     3241 cctggcagaa cgctgggcac tacggcgtgg tgtcgtgggg cgagaccgcg ggcgctgtca
     3301 ccgcagctgc gagccacgac aacgggcgca acaatgtcgc ggacccccgg ccgctgcggc
     3361 ttgaagagct cggcgaggcc ccgctcatcg tgtcgctgga tggaacctgg caccgcccgc
     3421 tgaccacgct cgagctcgcg gcgctccaag gcttcccgac cgaggggctg accttcgacg
     3481 gcagggcgat gcagaggcac cgcgagaaga tcggcaacgc tgtgccaccg ccggccgcgc
     3541 aggcgatagc cggcgtcatg gggcaggtgc tgctgatggc gcgcagcggg cagaccttcg
     3601 cgctgagcag cacgccagtg tgggtgcggc cggtggcgct ggcgctgacc gtagacgggg
     3661 aagccgtatg gacccgctga agcccttccc gcctactgtc gaatacatcc gcgacgggga
     3721 agatatgccc caagccttcc gcatcgcctg cgagcagcct gcttggagcc agctcctgcg
     3781 ctggcgacgg cgggggccga acctctaccc gcataccccg caccacgctg gcctgcctga
     3841 ccctgaacca ctcgatacga tcgtcacgat ggcgatgcgg ctgccacaag tcgagatgcg
     3901 gatcgtcctc ggctgcgaag acctcgagac catcgcggcc gagctccttc tgcggctgcg
     3961 gcccatcccc gagatcatgc gcggcagcat cgggatcatc gtcgaggcgt accgtcacga
     4021 cataggtctt accccgtggc gaccaggccg gcgcgggatc cgtggcgaag gcgtgcgcga
     4081 tatcgcacgg ctcgctgctg gtgatgcagc gctcaccgag aaacgcgcag cggtctatcg
     4141 ctggctgatg ggcctctgga tgccgccgga gcggtgggaa cccgaggtct tccaaggatt
     4201 ccggcaccac gaggtcacgt ttgtcgacgg gccgctctgg gcagcgtggg aactctcgcc
     4261 gatcggcgtc accgcgacag gggagtacgg cgtccaccct ggcgcgcgag cctggtaccg
     4321 aatcggccgc tgccgcttca aatccggcga ctctccagcc cgcatggtcg acgaggttct
     4381 acaaagaacg aagcgcttgt aaatttataa ttgacaagtt ctaggtagtg actaaactgc
     4441 actacatgaa ctcgattgct tcctccgaat cgcgcaaatc cgccgccctc ctcaccagcc
     4501 tgttcgaaga cctctgcacg ccaacggtga ctgtttctgt tggcgaagtc ggcatcgttg
     4561 tgtctgaaga gggattctgc actagcggcg cgatgggcac cgctgaggcg tcctggtctc
     4621 ctgtcgttat tgaaggcggc aagggcaagt ctgaaaccgt cgccgagcac atagtttctg
     4681 cggcgaagca gtacgattac aaatccgtcg gacagaagta cgatccgaag ctatctgtga
     4741 cggaaatcgc tgctcggatt cgcgcagaga tcaaggccgc gcagaagggc ggcgagctgc
     4801 cacgcagcat gaaagtgagc gttcgcaagc ggagctgcac ccatagctgg gcgatcgacg
     4861 tgtccgctac caacatcggc tgcgaactct acaccgacga ttacaagcgc gcccaggccg
     4921 agcatcgcga ctttccgtgg caccagcgct tcactgctga ggcagatcgc atcctcagca
     4981 agttgcaggc gatttggagc gcctacaact tcgacgacag cgacagcatg accgactact
     5041 tccacgtcaa ctactacggc ggcgtgaaaa tcgactgagg aaattatgac cccgatcaag
     5101 ctcaccaagt acacgattca gccgctcccc agcctggggc tgccgcttga ggtccaccgc
     5161 tacagctctg gcctcgtgct cgcggttcac gatgaccagc ccgacgtgcg ctttgcttcg
     5221 ctcgaggccc tctgcgatga gcaccacctg gacttgtcca agtacaagcg gctcgaggac
     5281 gtgtttgagc tgtcagtgag cgtcctcgag ttcggcacct atgccaaggg cgtctacgcg
     5341 ccagccggcg cgctgcatgg cttcgatccc gagcatccag aggtcgacta caacaccgag
     5401 cgcgcagacc ggctctatct cgcgctgggc atcgctccgg ataccggcga gcagctcggc
     5461 cgccttcctg atggccgctg ggcgctggtc acgaccgagg tgcctggcaa tcggttcgcg
     5521 atcgaagacg tggccacgac atgacggccg gcgagcgcat ccgcgcgcgc agaacagagc
     5581 tcaagctcac ccaggcccag ctcacccagg cgctcgggct gaagaaccgg cagagtgtga
     5641 accggatgga gctcgggcat caggcggtgt ctgccgagca agcggcgaag ctcgccccgc
     5701 tgctcaaggt ctcgccggcc tggctgctgt ttggggccgc cgatgaaagt tgacatcacc
     5761 gaccacgcca tcgaccgcta ccgcgagcgg atcgagccgg gcctgtccta cgaagaagcc
     5821 cgcgagcgca tcgccgaggg gatggagcgc gcgatccgcc tgcgcgagaa gacctatgcg
     5881 ggctcggata tgtggcacct cgccggcacc tgcgtcttcg tggtcgagcg ctgccgccac
     5941 gttcacaagc tgcacccggt gaagaccgtg atcactgagg ctgaggcgtt cgggcgcgac
     6001 gaagaggaga cacctgatgg caacgattaa ggtcagcggc gggaagtttt cctgccccag
     6061 cgaaatcacc ctcattgccg gtggtgagaa gttcgatctg cgcaaaatcc agctcggcat
     6121 ggacgaacca ctgctgccgc cgcttgagtt ccatacgatg ctggaattct gcgaccgcga
     6181 gatcaccaag gccgtcctcg gtcccttgct tcaagtcgaa gccccggctg tcgcgccttc
     6241 gatgatgcgt gcgctggtac atgcccacta caccgaggcg aagatgaagg aagactgggt
     6301 gcggttcgcc gccctctatg caattccctc gtgcgatcct cagaaaccca agcgcccgaa
     6361 gtggggtcag cggggccggc atcggctacg caagacccgg ctcgaggcgc gcgccatcaa
     6421 ccacctgatc aacgtcattc gaggagagct gctatgtcaa aaacccaagg ccaccctgtt
     6481 cacgatggaa acaagccacc cgccgacggt ggtgtgacaa agaaagccca ccgcggcttc
     6541 gctgcgatga agcccgagaa gcagaaggcc atcgccagca tgggcggcaa ggccgcgcac
     6601 gagcggggct acgcccacga gttcacggaa gaagaggccc gcctagctgg tcagaaaggc
     6661 gggcgcgcga tcagcgcaga ccgcgaccat atggcaagca ttggccggcg cggtgggctc
     6721 gctcggcacc agaaggccca gcaacccacg tagcgccggc atcgcggcgc ctgcgcgttc
     6781 ctggccccgc tgcgaaagcg gtggggcgcc agccctgaaa aagtttttct gggcgccttg
     6841 agggccgagc tctgacctcg gcctgacaca agtcgaggtt tctgacacga cttacacatc
     6901 agatcgaatc cgggtcgtgc gcgcaaatgc gcgcgcgtaa gcgcgagatt taa
//
LOCUS       DAOQNN010000150         6923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5934_length_6923_cov_6.973646, whole genome shotgun sequence.
ACCESSION   DAOQNN010000150 DAOQNN010000000
VERSION     DAOQNN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5934_length_6923_cov_6.973646"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PKV97_09325"
     CDS             <1..332
                     /locus_tag="PKV97_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB06114.1"
                     /translation="AGERVENSPRMLGRMNFSTPLANLPLRMGVEFAYDSKRRSPAGN
                     TVDAYWRSNLHLVAERWIQGAEVSLSVMNLFDEDYAHPSGGAPIHTMDRIAQDGRSLR
                     VKFDYRF"
     gene            339..890
                     /locus_tag="PKV97_09330"
     CDS             339..890
                     /locus_tag="PKV97_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNB06115.1"
                     /translation="MLSLSIRPPRRRLRAFILGALLAGVANLAHAGDITAEEYRVKAS
                     FLFNFAAFTFWPEGGGETLSICVYGGDPFGSHLDALMDRKVGERSVRVRRVGSVDALV
                     DCQLVFVPRQMISNLGRVLDGVDGQPVLTVADSPGALASGVVLNMDSSAGQIRFSVNL
                     AAARRQGLGLSSKLLNLATEIKR"
     gene            887..3652
                     /locus_tag="PKV97_09335"
     CDS             887..3652
                     /locus_tag="PKV97_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB06116.1"
                     /translation="MKMSHDEDDNRMTLGEKLRRVSQRALGAAVAMLAVAITLGSFLI
                     STLALESNTRVMARVLADNAAAGLMFLDHDAATRVLATLERLPDVRGAAIFDVSGAPF
                     ARYGKFIEQGRETVDFSAEHAHFSFETLHLTQPIVEGGNVVGVLDLDLDLRPLYLQVL
                     AHGSITVLAAAIALLLATHTLRRLNRAVLQPLSTLSALMERVAHTEDYSARAEPSRII
                     ELDSLASGFNRMLGQIAERDARLASHRDHLEDEVATRTEELLRAKEAAEAASQAKSDF
                     LATMSHEIRTPMNGVLGMTELLLDSGLNHEQRHFAEAVENSGRHLLGIINDILDFSKI
                     ESGHLELEAVEFELGDLLEDTLAMFAQPAEKKRLELIADLPPEVRRCVRGDPFRLRQI
                     VGNLANNAIKFTERGEVVVRARLLGEGTDGYRLRISVEDTGIGIPPHAQQKIFEQFSQ
                     LDGSTTRQFGGTGLGLAICRRLVGLMGGEIGVDSRPGAGACFHIDLCLPAGQSTAEAL
                     RCDVALERVHALVVDDNATNREILLRQLHGWGMQVECSADGAGALDRLARAATAGEGF
                     ELAVLDMHMPQMDGLQLATAIRANPALAGVRLVVLTSSYSSASVRERERAGILRCVHK
                     PIRQAELFEVLCSALRAGDAVAAATAVADEWRPTRGGRVLLAEDNPVNQQVGLAMLAK
                     LGIAVRVANDGEEAVKLAATEAFDLILMDCHMPVLDGYEASAAIRASEHAPRVPIVAL
                     TANVMEGNRERCLAAGMDDFLAKPYALEQLQSTLQRWMAATPASAPATPCAAATEAEA
                     TEVTEAAIDRTFIEQFRELDPDGGMALAARILRVYAESSAPPFAEIERATAAGDAAGL
                     RRAAHSLKSSAGNVGARRLAALLKDFESLGKDGRIDDARAGLAALRTEHARTLDAIHA
                     LIEEFGR"
     gene            3649..5760
                     /locus_tag="PKV97_09340"
     CDS             3649..5760
                     /locus_tag="PKV97_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB06117.1"
                     /translation="MSSTRILVADDDPTARLLMQAALGKSGFEVAVAEDGEEALQAFA
                     HATFDMVLLDVEMPRLDGYAVCRELRRRCGDALPIVMVTGMDDTASIEAAFEAGATDF
                     VAKPISWPLLGHRVRYVLRATRAATALRMANARNAAMLDAFPDTLLRLDAADRIVELR
                     AAPTGVPSCCMPAVGQTLAEAYPHEVATLLAQALDEARRSRQPSTVDFPLACRTGRPC
                     HQEARVSAIDDGEALCLLRDITERKEYETHIHHLAYFDSLTGLANRRSFLERLEREIR
                     RALQNKWRLAVLFLDLDRFKSINDTLGHAAGDALLQQAAERLRAITRPGDIVAHDTDR
                     MGTAEFARLGGDEFTVLLPTISRPDDALAIAERIRASICAPFAIEGQELGVTTSVGIA
                     VFPDDGEDAATLIKHADIAMYDAKANGRNGCRYYNPALTAEAVHRVELEAGLRRALAH
                     DELVLMYQPQVEVRDGSIATVEALVRWQTPDGRLVPPLEFIPFAEECGLIDAIGSWVL
                     RRACHDAAQWQRRGLDLRVAVNLSPVQFRASGLLDRIREALADSDLPAERLELEITES
                     ALMADDADTHATLAALRTLGIGIALDDFGTGYSSLSCLKRLPLDKLKIDRSFVSGLPD
                     DVDNHAIVRAILALARHLGFRITAEGVETAAQAELLGELEADSLQGYLFSRAVPAAEL
                     EALASRRWPPARGTQFRIDFS"
     gene            complement(5753..6268)
                     /locus_tag="PKV97_09345"
     CDS             complement(5753..6268)
                     /locus_tag="PKV97_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNB06118.1"
                     /translation="MSARNLFFGLALATSQGVLAQALPEFDLKATYAYNFAVLTQWPP
                     ATRASFNFCVYGDDGVAEAMRRLQGKSVHGRSVAVARLGALTAIRDCDLLYVGRDEAP
                     SLPHIAALLGDAPVLTVTDATQPQPVAVVIVPEGRRLAFEIDVERSRRAGLKPSAALL
                     GLARNLRQPLN"
     gene            complement(6265..>6923)
                     /locus_tag="PKV97_09350"
     CDS             complement(6265..>6923)
                     /locus_tag="PKV97_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB06119.1"
                     /translation="TTWKASYSEAFKMPNANDRWTSDDTAVPEYVAATELVLQRQLAP
                     HTRFTGSLYRYRRSDLPIENADGDEVPEGSSRARGVETEIEHVWERGARARASVAWQR
                     SRDVYGRDAVNSPDLLGKLAFTFLLPGEALRAGLETQYLGPRLTRERRMLGGVTLSNL
                     TLSTERDWHGLSASLSVRNLFDRDYETVSGFDWRPGDVAQDGLRMDGRSVWLQVGYAL
                     "
BASE COUNT         1103 a         2517 c         2301 g         1002 t
ORIGIN      
        1 cggcgggcga gcgcgtggag aactcgccgc gcatgctcgg ccggatgaac ttctccactc
       61 cgctggcaaa tctgccactg cgcatgggcg tggagttcgc ctacgacagc aagcgccgca
      121 gcccggcggg caacacggtc gacgcgtact ggcgttcgaa cctgcacctg gtggccgagc
      181 gctggatcca gggcgccgag gtgtcactga gtgtgatgaa cctgttcgac gaagactacg
      241 cccatccgag tggcggcgcg cccatccaca ccatggaccg catcgcccag gacggccgca
      301 gcctacgggt caagttcgac taccgcttct gacccagcat gctctcactt tccatccgcc
      361 cgccacgacg ccggctgcga gcgttcatcc tcggggcgct gctggctggc gtggcgaacc
      421 tcgcacatgc gggcgacatc accgccgaag agtacagggt caaggcatcc ttcctgttca
      481 acttcgccgc cttcaccttc tggccggagg gcggaggtga aaccctgtcg atctgcgtct
      541 atggtggcga cccgttcggc tcgcatctcg acgcgctcat ggaccgcaag gtgggcgaac
      601 gcagcgtgcg cgtacggcgt gtgggcagcg tcgacgccct cgtggactgc cagctcgtgt
      661 tcgtcccccg acagatgatc agcaacctgg gccgggtgct cgacggcgtg gacggccagc
      721 ccgtcctcac cgtggccgac agcccgggcg ccctggcgag cggcgtggtg ttgaacatgg
      781 acagcagcgc aggtcagatc cgcttttctg tcaacctcgc ggcggcccgc cgccaggggc
      841 tcgggctcag ctcgaagctc ctcaatctgg ccaccgaaat caagcgatga agatgtccca
      901 cgacgaagac gacaaccgca tgaccctcgg cgaaaagctg cgccgcgtaa gccagcgcgc
      961 gctcggcgcc gcggtggcga tgctcgcggt ggcgatcacc ctcggtagct tcttgatcag
     1021 cacgctcgcg ctcgagagca atactcgggt gatggcgcgg gtgcttgccg acaacgccgc
     1081 cgccggcctg atgttcctcg accacgacgc cgccacgcgc gtgctcgcga ccctggagcg
     1141 cctgccggac gtacgcggcg cagcgatctt cgatgtatcc ggagctcctt ttgcgcgtta
     1201 cggcaagttc atcgaacaag gccgggagac ggtggacttt tcggccgagc acgcgcactt
     1261 cagcttcgag acactgcacc tgacccagcc gatcgtcgag ggcgggaacg tggtcggggt
     1321 gctcgacctc gacctcgatc tgcgtccgct ctacctccag gtgctcgccc acggctcgat
     1381 tacggtgctc gccgcggcga tcgcgctgct gctcgccacc cacacgctgc gccggctcaa
     1441 ccgcgcggtg ttgcagccac tgtcgaccct gtccgcgctg atggagcgcg tcgcccacac
     1501 cgaggactac agcgcgcgcg ccgaacccag ccgtatcatc gagctcgatt ccctcgccag
     1561 tggcttcaac cgcatgctcg gccagatcgc cgagcgcgat gcacgcctgg caagccatcg
     1621 cgaccacctc gaggacgagg tcgccacacg caccgaggag ctcctgcgcg ccaaggaggc
     1681 cgcagaggct gccagccagg ccaagagcga cttcctcgcg accatgagcc acgagatccg
     1741 cacgccgatg aacggggtgc tcggcatgac cgagctgctc ctcgacagcg gcctcaacca
     1801 cgagcaacgc cacttcgccg aggcggtcga gaactccggt cgccacctgc tgggcatcat
     1861 caacgacatc ctcgacttct cgaagatcga atccggccac ctcgagctcg aagcggtgga
     1921 gttcgagctc ggcgacctgc tcgaggacac gctggcgatg ttcgcccagc cagccgagaa
     1981 gaagcgcctg gagctgatcg ccgacctgcc ccccgaggtg cggcgctgcg tgcgcggcga
     2041 ccccttccgc ctgcgccaga tcgtcggcaa cctggccaac aatgcgatca agttcaccga
     2101 gcgtggcgag gtcgtggtgc gtgcccgtct gctcggcgag ggcactgacg gctatcgcct
     2161 gcgcatcagc gtcgaggaca ccgggatcgg catcccgccc catgcgcagc agaagatctt
     2221 cgagcagttc tcccagctcg acggctccac cacccgccag tttggcggca ccggcctcgg
     2281 cctggcgatc tgccgccgcc tggtgggcct gatgggcggc gagatcggtg tcgacagccg
     2341 gcccggagcg ggcgcctgct tccatatcga cctgtgcctg ccggcggggc aaagcaccgc
     2401 cgaagccctg cgctgcgacg tcgcgctcga gcgtgtgcac gccctcgtcg tggatgacaa
     2461 tgccaccaac cgcgagatcc tgctgcgcca gctccacggc tggggcatgc aggtggaatg
     2521 ctctgccgac ggcgcgggcg cgctggatcg gctcgcacgc gccgccacgg ccggcgaggg
     2581 tttcgagctc gccgtactcg acatgcacat gccgcaaatg gacggcttgc agctggccac
     2641 cgcgatccgc gccaacccgg cgctcgccgg cgtgcgcctg gtggtgctga catcgagcta
     2701 ctccagcgcc agcgtgcgcg agcgcgaacg cgccggcatc ctgcgctgcg tgcacaagcc
     2761 gatccgccag gccgagctgt tcgaggttct atgcagcgca ttgcgcgctg gggacgcagt
     2821 ggcagcggcc accgcagttg cggacgagtg gcgaccgacg cgcggcgggc gcgtgctgct
     2881 ggccgaggac aacccggtga accagcaggt cggcctcgca atgctcgcca agctcggcat
     2941 cgcggttcgt gtcgccaacg acggcgaaga ggcagtgaag ctggctgcga ccgaagcctt
     3001 cgacctgatc ctgatggact gccacatgcc ggtgctggac ggctacgagg cgagcgccgc
     3061 aatccgcgca tcggagcacg cgccgcgcgt accgatcgtc gcgctcaccg ccaacgtcat
     3121 ggaaggcaac cgcgaacgct gcctggcggc cggcatggac gattttctcg ccaagcccta
     3181 cgcgctcgag cagctccaat ccaccctgca gcgctggatg gcggcaacgc ccgcgagcgc
     3241 gccggcgacg ccttgcgccg ccgcgaccga agccgaggcg accgaggtga cagaggcggc
     3301 gatcgatcgg accttcatcg agcagttccg cgagctcgac cccgacggcg gcatggcgct
     3361 cgccgcgcgc atcctccgcg tctatgccga gagcagcgcc ccccccttcg ccgagatcga
     3421 gcgcgccacc gccgccggcg acgccgcggg actgcgtcgc gccgcgcaca gcctcaagtc
     3481 gagcgcgggg aacgtcggcg ccagacgcct cgcggccctg ctcaaggact tcgagagcct
     3541 cggcaaggac ggccggatcg acgacgcgcg cgccggcctc gccgcgctgc gcaccgagca
     3601 tgcgcgcacg ctcgacgcca tccatgccct gatcgaggag ttcggtcgat gagctcgact
     3661 cgcatcctgg tcgccgacga cgatccgacc gcacgcctgc tgatgcaagc cgcgctcggc
     3721 aagagcggct tcgaggtcgc cgtcgccgag gatggcgagg aggccttgca ggccttcgcg
     3781 cacgccacct tcgacatggt gctgctcgat gtggaaatgc cgcgcctgga cggctacgcg
     3841 gtctgccgcg agctgcgccg ccgctgtggc gacgcgctgc cgatcgtgat ggtcaccggc
     3901 atggacgaca ccgcatcgat cgaggccgcc ttcgaggccg gcgcgaccga cttcgtcgcc
     3961 aagccgatca gctggccgct tcttggccac cgcgtccgct acgtgctgcg cgccacccgc
     4021 gccgccaccg cgctgcgcat ggccaacgcg cgcaacgcgg ccatgctcga cgccttcccc
     4081 gacaccctgc tgcgcctcga cgcggccgac cgcatcgtcg agctgcgcgc agccccgacc
     4141 ggcgtgccca gctgctgcat gccggcggtc ggccagaccc tggccgaagc ctatccgcac
     4201 gaggtggcga cgctgctcgc gcaagcgctc gacgaggccc gccgcagccg gcagccgagc
     4261 acggtggatt ttcctctcgc gtgtcggacc ggacgcccct gccaccagga agcacgggtg
     4321 agcgcaatcg acgacggcga ggccctctgc ctgctgcgcg acatcaccga gcgcaaggag
     4381 tatgagaccc acatccacca cctcgcctat ttcgacagcc tgaccgggct ggcgaaccgg
     4441 cgcagcttcc tggagcggct cgagcgcgag atccgccgcg cccttcagaa caagtggcgc
     4501 ctggcggtgc tcttccttga tctggaccgt ttcaagagca tcaacgacac cctcggccac
     4561 gctgcagggg acgccctgct gcagcaggcc gccgagcgcc tgcgtgcgat cacccgcccg
     4621 ggcgacatcg tggcgcacga cactgatcgc atgggcacgg ccgaattcgc tcgcctcggc
     4681 ggcgacgaat tcaccgtgct gctgccgacg atctcccgac cggacgacgc gctggcgatc
     4741 gccgagcgca tccgcgccag catatgcgct ccattcgcga tcgagggcca ggagctcggc
     4801 gtcaccacca gcgtcggcat cgccgtcttc ccggacgacg gcgaggacgc cgccacgctg
     4861 atcaagcacg ccgacatcgc gatgtacgac gccaaggcca acggacgcaa cggctgccgc
     4921 tactacaacc ctgcgctgac cgccgaggcg gtgcatcgcg tggaactcga ggccgggctt
     4981 cgccgcgcgc tcgcccacga cgagctcgtg ctgatgtacc agccgcaggt ggaggtgcgc
     5041 gacggcagta tcgccaccgt cgaggccctg gtgcgctggc agacgcccga cggcaggctg
     5101 gtgccgccgc tcgagttcat ccccttcgcc gaggaatgcg gcctgatcga cgccatcggc
     5161 tcctgggtgc tgcgtcgcgc ctgccatgac gcagcacaat ggcagcgccg cgggctcgac
     5221 ctgcgcgtcg cggtcaatct gtcgccggtg cagttccgcg ccagcgggct gctcgacagg
     5281 atccgcgagg ccctcgccga cagcgacctg cccgccgaac ggctggagct cgagatcacc
     5341 gagagtgcgc tgatggccga cgatgccgac acccatgcca cgctggccgc actgcgcacg
     5401 ctcgggatcg gcatcgcact cgacgacttc ggcaccgggt actcgtcact gagctgcctc
     5461 aagcgcctgc cgctggacaa gctgaagatc gaccgcagct tcgtcagcgg cctgcccgac
     5521 gacgtcgaca accacgcgat cgtgcgcgcg atcctcgcgc tcgcacgtca cctgggcttc
     5581 cgcatcacgg ccgagggcgt cgagaccgcg gcgcaggccg agctgctcgg cgagctcgag
     5641 gccgacagcc tgcagggcta cctgttcagc cgcgcggtgc cggccgcgga gctcgaagcg
     5701 ctggcctcgc gcaggtggcc gccggcgcgt ggcacccagt tccgcatcga cttcagttga
     5761 gcggctggcg caggttgcgc gcgagcccga gcagcgccgc gctgggcttg agcccggcac
     5821 ggcggctgcg ctcgacatcg atctcgaagg ccaggcgccg gccctcgggc acgatcacca
     5881 cagcgaccgg ttgcggctgc gttgcgtcgg tcacggtcag caccggcgca tcgccgagca
     5941 aggctgcgat gtgtgggagg ctgggcgcct cgtccctgcc gacgtaaagc aggtcgcagt
     6001 cacggatcgc ggtcagtgca ccgagccgcg ccaccgccac gctgcgccca tgcacggact
     6061 tgccctgcag ccggcgcatc gcctcggcca cgccgtcgtc cccatagacg cagaagttga
     6121 agctcgcgcg cgtcgccggc ggccactgcg tgagcacggc gaagttgtag gcgtaggtgg
     6181 ccttgaggtc gaactcgggc agcgcctgtg cgagcacgcc ctgcgaagtc gccagcgcga
     6241 gcccgaaaaa caggttgcgt gcgctcatag cgcgtacccg acctgcagcc agacgctgcg
     6301 tccgtccatg cgcaggccgt cctgtgccac gtcaccgggc cgccagtcga agcccgacac
     6361 ggtctcgtag tcacgatcga acaggttgcg cacgctcagc gaggccgaca ggccatgcca
     6421 gtcgcgctcg gtggacaggg tcagattgga aagcgtcacc ccgcccagca tgcgccgctc
     6481 gcgggtcagg cgcgggccga gatattgcgt ctcgaggccg gcacgcagcg cctcgcccgg
     6541 cagcaggaag gtgaaggcga gcttgccgag caggtcgggc gagttgaccg cgtcacgccc
     6601 gtacacatcg cgcgagcgct gccaggccac gctggcgcgc gcacgcgccc cgcgctccca
     6661 gacatgctcg atctcggtct cgacgccgcg cgcgcggctg ctgccctcgg gaacctcgtc
     6721 cccgtccgcg ttctcgatcg gcaggtcgct gcgccggtag cggtacaggg agccagtgaa
     6781 gcgcgtgtgc ggcgcgagct ggcgctgcag cacgagctcg gtggcggcga cgtactcggg
     6841 gacggccgtg tcgtcggacg tccaccgatc gttggcgttg ggcatcttga aggcctcgct
     6901 gtacgaagcc ttccaggtcg tcg
//
LOCUS       DAOQNN010000151         6894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_5979_length_6894_cov_7.537944, whole genome shotgun sequence.
ACCESSION   DAOQNN010000151 DAOQNN010000000
VERSION     DAOQNN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5979_length_6894_cov_7.537944"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKV97_09355"
     CDS             complement(<1..304)
                     /locus_tag="PKV97_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNB06120.1"
                     /translation="MAHVSDLIATDIEQYLRAHENKSLLRFITCGSVDDGKSTLIGRL
                     LYESKMLFEDQLAAVEADSKKYGTQGEAIDFALLVDGLAAEREQGITIDVAYRFFST"
     gene            complement(305..1222)
                     /gene="cysD"
                     /locus_tag="PKV97_09360"
     CDS             complement(305..1222)
                     /gene="cysD"
                     /locus_tag="PKV97_09360"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNB06121.1"
                     /translation="MTSVPTTSLTHLQRLEAESIHILREVVAEADNPVMLYSIGKDSA
                     VMLHLALKAFHPAVPPFPLLHVDTRWKFRDMYSFRDRMAGETGMDLLVHINPEGVEKD
                     INPFTHGSAIHTDIMKTEGLKQALDQYGFDVAFGGARRDEEKSRAKERVFSFRTAQHR
                     WDPKNQRPELWKLYNARKHKGESIRVFPLSNWTELDIWQYIYLENIPIVPLYYAAVRP
                     VVERDGTLIMVDDERMPLRPGEVPMMKKVRFRTLGCYPLTGAIESEADTLPAIIQEML
                     LARTSERQGRVIDHDSAASMEKKKQEGYF"
     gene            1417..2187
                     /gene="cysQ"
                     /locus_tag="PKV97_09365"
     CDS             1417..2187
                     /gene="cysQ"
                     /locus_tag="PKV97_09365"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNB06122.1"
                     /translation="MDTATASRLLEALLPIVRAAGALVMDIYRSDFAVRGKDDASPVT
                     EADERAEAHIVPALETLLPGVPVVAEEAVAAGRLPALGRRFWLVDPLDGTKEFIGRNG
                     EFTVNIALVEDGEPVLGTVFAPALERLFLGAGGVGAFVEQDGRRRPIRCRTVPPAGLT
                     VVASRSHGDAAALDAFLDGRKVAALTNAGSSLKLCLVAAGEADLYPRLGRTMEWDIAA
                     GHAVLAAAGGRVQTLAGAPLRYGKPGLDNPHFVASGLD"
     gene            2202..3938
                     /locus_tag="PKV97_09370"
     CDS             2202..3938
                     /locus_tag="PKV97_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNB06123.1"
                     /translation="MTTAHLVLASIALLLALLAHGRVGPAVLFAAWAGGYHLLGLASQ
                     QELLASFSNPALATLILLLLVSLALERSPLLDHLSELLLKGRVGLATLRLSVFACLIS
                     AFMNNTAVVGALLGTVTSQRRHPASKLLIPLSYASILGGITTLVGTSTNLVVNSFVID
                     AGLPPLQMFQFAWVGIPVALACIGVMALSSRLLPANASSEAEARSAYFLEARVAAGSP
                     MIGRSIETNRLRNLEGLFLLEIEREGRLISPVGPAEILHTGDVLVFTGEVARVQTLQR
                     FPGLEVFGAGADALLKSNLVEVVLASSSDLANRTLREVDFRTMFDAGVVGIRRGDRRL
                     TGQLGRIPLRVGDSLLLAAGPDFLQHRNLDRNFHILNGSGLRPKLGRMQSVAALSGFA
                     LVILVAALGWLPLFGGLLVLLAALLGSGLLTLGELRRRFPFELLVVIGSALAIAGVVE
                     RSGAAELVAGWMRAIFDGHGVHAALAGVYLITLTLTEVITNTAAAALAFPIALGTARA
                     FGVDPMPFVMVVAYGASAGFLIPFGYQTHLMVYSAGRYRMRDFLRAGLPVSLTYSALV
                     LLLVPLVFPLHR"
     gene            complement(3954..4220)
                     /locus_tag="PKV97_09375"
     CDS             complement(3954..4220)
                     /locus_tag="PKV97_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNB06124.1"
                     /translation="MSEQILKEVLSLLDEVLSLDGRALAFAADTPLLGDLPELDSMAV
                     IAVIQEIEARFGIEVPDDEVDGNSFATVGTVVEFVTRLSARQPG"
     gene            4332..5924
                     /locus_tag="PKV97_09380"
     CDS             4332..5924
                     /locus_tag="PKV97_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HNB06125.1"
                     /translation="MHAKHLLHHLVESAAARDPARIAVVAGDRQLRYQELEAALHAFA
                     GGLAGLGLARAERVGIYLDKRPEFVAAAFGTSAAGGVFVPINPVLKAAQVGHILRDCA
                     VRVLVTTAERLAALGDALDHCHDLRHVVLVGADGATTRSVAVHSWQALLAAPARAGHR
                     VIDADMAAILYTSGSTGRPKGVVLSHRNMMAGARSVAHYLGNHPDDTLLAALPLSFDA
                     GLSQLTTAFQAGARVVLLNWLLPRDVLKAIERERVSGLTAVPPLWIQLAQMPWPTGTD
                     EHLRYIANTGGRMPGEVLARLRAQLPKTRPFLMYGLTEAFRATYLPPEELDRRPDSIG
                     KAIPDAEVLVLREDGSECAPNEPGELVQRGALVAMGYWNDPERSAERFRPLPAAAPGR
                     EPGLVLPEIAVFSGDTVRRDEDGYLYFIGRRDEMIKTSGYRVSPTEIEEILYATGLVG
                     ECAAFGVAHASLGQSIVAVVTPPAGATLDTAALLAECRSRMPAYMVPARFEPRSGALP
                     RNANGKIDRKALADALAAGEPA"
     gene            5921..>6894
                     /locus_tag="PKV97_09385"
     CDS             5921..>6894
                     /locus_tag="PKV97_09385"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNB06126.1"
                     /translation="MNAPARSLPTHAPQTVFAIADGELQIAGQPLSRLAARVGRTPFY
                     AYDRGAIARRVAELRAALPPAIELHYAMKANPMPALVGHLARLVDGIDVASAGELQVA
                     LDAGADPQHVSFAGPGKRDTELRQAVAAGILVNVESFREIAPLAEAAAALGVPARVAV
                     RVNPDFELKSSGMKMGGGPKPFGVDAEQVPGLLAEIGRAGLAFEGFHLFAGSQNLKAE
                     AIVEAQRKSFELALRLAAHAPAPVRTLNLGGGFGIPYFPGEQALDLTPIAAGLADIVA
                     EAAQALPEARIVLELGRYLVGEAGVYVCRITDIKVSRGHTYLVTDGGL"
BASE COUNT          934 a         2591 c         2290 g         1079 t
ORIGIN      
        1 cggtggagaa gaagcggtag gccacgtcga tcgtgatgcc ctgctcgcgc tcggcggcca
       61 ggccgtcgac cagcagcgcg aagtcgatcg cctcgccctg ggtgccgtac ttcttcgagt
      121 cggcctcgac cgcggcgagc tggtcctcga agagcatctt cgactcgtag agcaggcgcc
      181 cgatcagcgt gctcttgccg tcgtccacgc tgccgcaggt gatgaagcgc agcaggctct
      241 tgttctcgtg cgccctgagg tactgctcga tgtcggtcgc gatcaggtcg gaaacgtgtg
      301 ccatttagaa atagccctcc tgctttttct tttccatcga ggcggccgag tcgtggtcga
      361 tcacccgccc ctggcgttcc gaggtcctgg cgagcagcat ctcctggatg atcgccggca
      421 gggtgtcggc ctcggactcg atcgcgccgg tgagcggata gcagcccagg gtgcggaagc
      481 gcaccttctt catcatcggc acctcgcccg ggcgcagcgg catgcgctcg tcgtcgacca
      541 tgatcagcgt cccgtcgcgc tcgaccaccg ggcgcaccgc ggcgtagtag agcggcacga
      601 tggggatgtt ctcgaggtag atgtactgcc agatgtcgag ctcggtccag ttcgacagcg
      661 ggaacacgcg gatcgactcg cccttgtgct tcctcgcgtt gtagagcttc cacagctcgg
      721 ggcgctggtt cttcgggtcc cagcggtgct gcgcggtgcg gaaggagaac acgcgctcct
      781 tggcgcgcga tttctcctcg tcgcggcgcg cgccgccgaa ggccacgtcg aagccgtact
      841 ggtcgagcgc ctgcttgagg ccctcggtct tcatgatgtc ggtgtggatc gccgagccgt
      901 gggtaaaggg gttgatgtcc ttctccacgc cctcggggtt gatgtgcacc agcaggtcca
      961 tgccggtctc gcccgccatg cggtcgcgaa agctgtacat gtcgcggaac ttccagcgcg
     1021 tatcgacgtg cagcagcgga aagggcggca ccgccggatg gaaggccttc agggccaggt
     1081 gcagcatcac cgcgctgtcc ttgccgatgg agtagagcat caccgggttg tcggcctcgg
     1141 cgacgacctc gcgcaggatg tggatgctct cggcttcgag acgctggagg tgggtcaggc
     1201 tggtcgtagg cacgctggtc atgggcggcg ggatgcgaag ggacaaggca tgaatgatga
     1261 ggccgcgcgc cgtcgcgcgc agcgtcttcg aagatcggcg ggcggaacta tttctcgcgg
     1321 tgcgacattc tgcacccgaa tccgcctctc gacggggctc tcgccacgcc cgcccgggta
     1381 cacttgcggc gtcgaccact cccccggagc ccgctgatgg acaccgcaac cgcctcacgg
     1441 ctcctcgaag ccctgctccc gatcgtgcgc gcggccggcg cgctggtgat ggacatctac
     1501 cgcagcgact tcgcggtgcg tggcaaggac gacgcctcgc cggtgaccga ggccgacgag
     1561 cgcgccgagg cgcacatcgt ccccgccctc gagaccttgc tgcccggcgt gccggtggtg
     1621 gccgaagagg ccgtcgcggc gggccgcctg cccgcactcg ggcgccgctt ctggctggtc
     1681 gatcccctcg acggcaccaa ggagttcatc ggccgcaacg gcgagttcac cgtcaacatc
     1741 gccctcgtcg aggatggcga gccggtcctc ggcaccgtct tcgcacccgc gctcgagcgc
     1801 ctcttcctcg gcgcgggcgg ggtcggcgcc ttcgtcgagc aggacggccg tcgccgcccg
     1861 atccgctgcc gcaccgtccc gcccgccggc ctcaccgtgg tggccagccg ctcgcacggc
     1921 gacgcggccg cgctcgacgc cttcctcgac gggcgcaagg tggccgcgct caccaacgcc
     1981 ggctcctcgc tcaagctgtg cctggtcgcg gcgggcgagg ccgatctcta tccgcgcctc
     2041 ggccgcacca tggaatggga catcgccgcc ggccatgcgg tgctggccgc cgccggcggc
     2101 cgcgtgcaaa ccctggcggg ggcgccgctg cgctacggca agccggggct ggacaacccc
     2161 cacttcgtcg ccagcggact ggactgagcc cgggcgcgcc gatgacgacc gcccacctcg
     2221 tcctcgcctc gatcgcgctg ctgctggcgc tgctggccca cggccgcgtc ggcccggcgg
     2281 tgctgttcgc ggcctgggcg ggcggctacc acctgctcgg cctcgccagc cagcaggaac
     2341 tgctggcgag cttctccaac cccgcgctcg ccaccctgat cctgctgctg ctggtctcgc
     2401 tcgcgctcga acgctcgccg ctgctcgacc atctgtcgga actcctgctc aagggccgcg
     2461 tcgggctggc gaccctgcgt ctgtccgtct tcgcctgcct gatctcggcg ttcatgaaca
     2521 acaccgcggt ggtgggcgcg ttgctgggca cggtcacctc gcaacgccga caccccgcct
     2581 cgaagctgct gatcccgctc tcctacgcct ccatcctcgg cggcatcacc acactggtcg
     2641 gcacctcgac caacctggtg gtgaactcct tcgtcatcga cgcgggcctg ccgccgctgc
     2701 agatgttcca gttcgcctgg gtcgggattc cggtcgcgct cgcctgcatc ggggtgatgg
     2761 cactgagctc gcgactgctg cccgcgaacg cctcgagcga ggctgaggca cgctcggcct
     2821 acttcctcga ggcgcgcgtg gctgcgggct caccgatgat cggacgcagc atcgaaacca
     2881 accgcctgcg caacctcgag ggcctgttcc tgctcgagat cgagcgcgag ggccgactga
     2941 tctcgccggt cggcccggcc gagatcctgc acaccggcga cgtgctggtg ttcaccggcg
     3001 aggtggcgcg ggtgcagacc ctgcagcgct tccccgggct ggaggtgttc ggcgcgggcg
     3061 ccgacgcgct gctcaagtcc aacctggtcg aggtggtgct ggcgagcagc tcggacctgg
     3121 ccaaccgcac cctgcgcgag gtcgacttcc gcaccatgtt cgatgccggg gtggtcggca
     3181 tccgccgcgg cgaccggcgc ctgaccggcc agctcggacg catcccgctg cgcgtcggcg
     3241 acagcctgct gctggccgcg ggcccggact tcctgcagca ccgcaacctc gaccgcaact
     3301 tccacatcct caacggctcg ggtctgcgcc ccaagctcgg ccgcatgcaa agcgtcgcgg
     3361 cgctgtcggg cttcgcactc gtgatcctgg tcgccgccct gggctggctg cccctgttcg
     3421 gcggcctgct cgtgctgctc gccgccctgc tcggcagcgg cctgctgacc ctgggcgagc
     3481 tgcgccggcg ctttcccttc gaactgctgg tggtgatcgg ctccgcgctg gcgatcgccg
     3541 gcgtggtcga gcgttcgggc gcggcggagc tggtcgcggg ctggatgcgg gcgatcttcg
     3601 acgggcacgg cgtccatgcc gcgctcgcgg gcgtgtacct gatcacgctg acgctcaccg
     3661 aggtgatcac caacaccgcc gccgccgcgc tcgccttccc gatcgcgctc ggcaccgcgc
     3721 gcgcgttcgg ggtcgatccg atgcccttcg tgatggtggt ggcctacggc gcgagcgccg
     3781 gcttcctgat ccccttcggc taccagaccc acctgatggt gtattcggcc ggccgctacc
     3841 gcatgcggga tttcctgcgc gccggactgc cggtgagcct cacctactcg gcgctggtgc
     3901 tgctgctggt gccgctggtg tttccgctcc accgctgagc acggcgcacg gaatcagccg
     3961 ggctggcgag cactcaggcg ggtgacgaac tcgaccacgg taccgaccgt ggcgaaggag
     4021 ttgccatcga cctcgtcgtc cggcacctcg atgccgaaac gcgcctcgat ctcctggatg
     4081 acggcgatca ccgccatcga atcgagctcg ggcagatctc ccagcaaggg cgtgtctgcg
     4141 gcgaatgcga gcgcgcgacc gtccaggctc agcacctcgt ccagcagcga cagcacttcc
     4201 ttcagaatct gttcggacat cacttctcca tgacggaagc gggtcacgac gcgcatccac
     4261 gccgcccgcc ccgcaaaacg cgcgcattat acttgccagc ccgttcctgt ctgcaccgat
     4321 aagcgaagcc gatgcacgcc aaacacctgc tccaccacct cgtcgagtcc gcggccgcac
     4381 gcgatcccgc acgcatcgcc gtggttgccg gcgaccggca gctgcgctac caggagctcg
     4441 aggccgcgct ccacgctttc gccggcggcc tggccgggct gggcctcgcc cgcgccgagc
     4501 gcgtcgggat ctatctcgac aagcgccccg agttcgtcgc cgccgccttc ggcaccagcg
     4561 ccgcgggcgg cgtcttcgtg ccgatcaacc ccgtgctcaa ggccgcgcag gtcggccaca
     4621 tcctgcgcga ctgcgcggtc cgcgtcctcg tcaccaccgc agaacgcctc gccgcgctcg
     4681 gcgacgcgct cgaccactgc cacgatctgc gccacgtggt gctggtgggc gcagacggcg
     4741 cgaccacccg ttccgtcgcc gtgcattcct ggcaggccct gctcgccgcc ccggcacgcg
     4801 ccgggcatcg cgtcatcgac gccgacatgg cggcgatcct gtacacctcg ggcagcaccg
     4861 ggcgaccgaa aggcgtggtg ttgtcccacc gcaacatgat ggcgggggcg cgcagcgtgg
     4921 cgcactacct cggcaaccac cccgacgaca ccctgctcgc cgccctgccg ctgtccttcg
     4981 acgccgggct gtcgcagctc accaccgcct tccaggccgg cgcccgcgtc gtgctgctca
     5041 actggctgct gccgcgcgac gtgctcaagg cgatcgagcg cgaacgcgtg agcgggctga
     5101 ccgcggtgcc gccgctgtgg atccagctcg cgcagatgcc ctggccgacg ggtacggacg
     5161 agcacctgcg ctacatcgcc aacaccggcg gacgcatgcc gggcgaggtc ctcgcccgcc
     5221 tgcgcgcgca gttgccgaag acccgcccct tcctgatgta cggcctcacc gaggccttcc
     5281 gcgcgaccta cctgccgccc gaggagctcg accgccgccc cgactcgatc ggcaaggcca
     5341 tccccgacgc cgaggtgctg gtgctgcgcg aggacggcag cgaatgcgcg cccaacgagc
     5401 cgggcgagct cgtgcagcgc ggcgccttgg tggcgatggg ctactggaac gaccccgagc
     5461 gcagcgccga gcgcttccgc ccgctgcccg ccgccgcccc cggccgcgaa cccggcctgg
     5521 tgctgcccga gatcgcggtg ttctccggcg acacggtgcg ccgcgacgag gacggctacc
     5581 tctacttcat cggccgtcgc gacgagatga tcaagacctc gggctaccgc gtcagcccca
     5641 ccgagatcga ggagatcctc tacgccaccg gcctggtcgg cgaatgcgcg gccttcggcg
     5701 tcgcacacgc gagcctcggc cagagcatcg tcgcggtcgt caccccaccc gccggcgcca
     5761 cgctcgacac tgccgcactg ctggcggagt gccgcagccg catgccggcc tacatggtgc
     5821 cggcgcgctt cgagccgcgc agcggcgcgc tgccgcgcaa cgccaacggc aagatcgacc
     5881 gcaaggcgct cgccgacgcc ctcgccgccg gagagcccgc atgaacgccc ccgcccgcag
     5941 cctgcccacg cacgcgccgc agaccgtgtt cgcgatcgcc gacggcgaac tccagatcgc
     6001 tggccagccg ctctcccggc tcgccgcgcg cgtcgggcgg acgcccttct acgcctacga
     6061 tcgcggcgcg atcgcacgcc gggtggccga gctgcgcgcg gcactgccgc ccgccatcga
     6121 gctgcactac gcgatgaagg ccaacccgat gccggcgctg gtgggccacc tggcgcgcct
     6181 ggtcgacggc atcgacgtcg cctccgccgg cgagctgcag gtcgcgctcg acgccggtgc
     6241 cgacccccag cacgtcagct tcgccggtcc cggcaagcgc gacaccgagc tgcgccaggc
     6301 ggtggccgcc ggcatcctgg tcaatgtgga atccttccgc gagatcgcgc cgctggccga
     6361 ggccgcggcc gcgctcggcg tgccggcgcg ggtcgcggta cgggtgaatc cggacttcga
     6421 gctcaagtcc tcgggcatga agatgggcgg cggccccaag cccttcggcg tggacgccga
     6481 gcaggtaccc ggcttgctcg ccgagatcgg ccgcgccggg ctcgccttcg agggcttcca
     6541 cctcttcgcc ggctcgcaga acctcaaggc cgaggccatc gtcgaggccc agcgcaagag
     6601 cttcgaactc gccctgcgcc tggccgcgca cgcccccgcc ccggtgcgca cactgaacct
     6661 cggcggcggc ttcggcatcc cgtacttccc cggcgaacag gcgctcgacc tgacgccgat
     6721 cgccgccggc ctcgccgaca tcgtcgccga ggccgcacaa gccctgcccg aggccaggat
     6781 cgtgctggaa ctcggccgct acctggtcgg cgaggccggc gtgtatgtgt gccggatcac
     6841 cgacatcaag gtctcgcgcg gccacaccta tctcgtcacc gacggcggcc tgca
//
LOCUS       DAOQNN010000152         6833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6077_length_6833_cov_10.830038, whole genome shotgun sequence.
ACCESSION   DAOQNN010000152 DAOQNN010000000
VERSION     DAOQNN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6077_length_6833_cov_10.830038"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1066)
                     /gene="ispB"
                     /locus_tag="PKV97_09390"
     CDS             complement(98..1066)
                     /gene="ispB"
                     /locus_tag="PKV97_09390"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HNB06127.1"
                     /translation="MSVQQLFAPIAADMQAVDTVIRNRLHSDVALIRRIAEYIVGSGG
                     KRLRPALVLYAAGALDYRGVHHHELAAVVEFIHTASLLHDDVVDESDLRRGKRTANAA
                     FGNASAVLVGDFLYSRAFQMMVGVDEMRVMRVLADATNIIAEGEVLQLLNCHNADVVI
                     EDYLRVIRYKTAKLFEAAARLGGIVAGADDALEQRLAAFGMHLGTAFQLIDDVLDYSA
                     DEADTGKHLGDDLAEGKPTLPLIHVMQHGTAEQGALVRHAIEGGGRGDFAAVLAAIQS
                     TGALEETRRYAEAEAKLAIDAISVLPPSIFKEALLQLSDFAVRRKH"
     gene            complement(1162..2424)
                     /locus_tag="PKV97_09395"
     CDS             complement(1162..2424)
                     /locus_tag="PKV97_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNB06128.1"
                     /translation="MNKMRFKALAAAVAAGGLFFAPGSVVQASAQQQVQLFHRLPDAK
                     AGALKDLVERFNAQSKDVQVVMSAADWRSGAPHLMILEGDDEEEFVAGKPRFKPLFQL
                     MKESGVPLQTLRPPAMMTRTPVDAKGQLLALPVGLSTPVLFLNRDALRQAGLNPETTR
                     INTWFDLQETLGRLADTGHTCPYTVAEPGRVMVENLSAWHNEPVAAQSGKTTVPSFNG
                     MFQVKHVAMMASWTRARYLHVFDQQAEAEQRFARGECAVIAAPSASWTDFRRAGKVDV
                     AVSKLPYYDDFPGAPQNTIADGPALWASAGKKPAEYKAVARFVSFWLQPDNQVAWQRE
                     TGYLPLNRAGLLASRSELLGNDLENIQVAVDQLGGKPATPQSSAQPVVERQKVRRILD
                     EELAGVWADEKAAKEALDNAVTRARNGN"
     gene            2603..2965
                     /locus_tag="PKV97_09400"
     CDS             2603..2965
                     /locus_tag="PKV97_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06129.1"
                     /translation="MPRPTPSPTLRRLVLLALLPFAAGCDQLAEVLELPNPSKVEAEG
                     RAVGSACRHSGRSLEDCYALNPAASKAAVFAGWRDMNDYMMEHKLEIVPSQLVQGAPK
                     AAPPRSTAQAAPAQPAAH"
     gene            complement(2987..4024)
                     /locus_tag="PKV97_09405"
     CDS             complement(2987..4024)
                     /locus_tag="PKV97_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB06130.1"
                     /translation="MEYRRLGGAGPQVSAVCLGTMTFGQQNSEAEAHSQLDLAVERGI
                     DFIDTAEMYPVPARAETSGETERIVGNWLARQVREKLVIATKVAGPARSLEWIRGGPL
                     ALDRANIRTAVEGSLRRLRTDYIDLYQLHWPARNQPLFGQLPFDPARERESTPIRAQL
                     EALAELVDEGKIRHVGLSNEQPWGLMEFVRMAQESGLPRVVSVQNAYNLLNRVYEYGM
                     SEITLREDVALLAYSPLAFGHLSGKYLADPAARGRLTEFENFGVRYAKPGVRAAVERY
                     AEIARRRGMSLTALALAFVYSRWFVGSTIVGATTVAQLAENLDAWHRRLDEDALAEIE
                     RVHQLHGNPAP"
     gene            4324..5418
                     /gene="rlmM"
                     /locus_tag="PKV97_09410"
     CDS             4324..5418
                     /gene="rlmM"
                     /locus_tag="PKV97_09410"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HNB06131.1"
                     /translation="MNQHTPAAALACAGLLGYCRAGFEKELAAELDDIAAEAGLIGYV
                     RAEPDSGYVIYETFEPTPLGSFGESTDWRRPVFARQLLPWFARVDDLPERDRATPIVE
                     AVKASGQRFSGVMLETPDTDEAKQRSGFCKRFTEPLAKALEKAGCLRSSRAGLPVLHV
                     LFTSATTAWLAAGQPGQCSTWPMGIPRLRMPSNAPSRSTAKLSEAFMMLLEEGERDSI
                     LRAGQRAVDLGAAPGGWTWQLVNRGLRVTAIDNGPLRDSVMATEMVEHLKADGFTWRP
                     HRPVDWMVCDMVEQPSRIASLMAEWVATGRCRYTIFNLKLPMKRRVEAVEQCRELIRK
                     RLASVGPYDLRIKHLYHDREEVTAFLTLKR"
     gene            5557..6804
                     /locus_tag="PKV97_09415"
     CDS             5557..6804
                     /locus_tag="PKV97_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB06132.1"
                     /translation="MSFADLGLIPELLQAVTDAGYTEPTPIQRQAIPIVIAGKDVMGG
                     AQTGTGKTAGFTLPLLHRIARHANTSTSPARHQTRALILAPTRELAMQVFESVKTYSK
                     HLPLRSVCVYGGVDIRPQQAELRRGIEVVIATPGRLLDHLEQKSINLSQVEVLVLDEA
                     DRMLDMGFIPDIKRILALLPKQRQSLLFSATFSDEIKRLADQMLKDPQLIEVARRNMV
                     SETITHVVHPVSSGMKRNLLAHLLRHKPDTQALVFVDTKLMCGRLAHYLERSGISADA
                     IHGDKGQQQRTDTLEAFKSGKLRVLVATDVAARGLDIDELPFVINFELPHTAEDYVHR
                     IGRTGRAGHHGYAISLVSSEEKHWLSEIEKLIKLQIPQERVPGFDPDPDFHEAGSRGH
                     RGRRPAAAAADTEAQAPAARSGW"
BASE COUNT         1057 a         2474 c         2261 g         1041 t
ORIGIN      
        1 ccggcccctg cgtcccgaac gcagtgctct accaggctga gctattcccc gacacttttt
       61 tgctgctttt tgctgccctg cgaagccgga aactatatca gtgttttcgc cgaactgcaa
      121 agtccgataa ttgtagcagc gcttccttga aaatggaagg gggaagcacc gaaattgcgt
      181 cgattgcgag cttcgcctcc gcttccgcgt agcggcgcgt ctcttcgagc gcgccggtcg
      241 actggatcgc cgcgagcacg gccgcgaaat cgccgcggcc gccgccttcg atcgcgtgac
      301 gcaccagcgc gccctgctcc gccgtgccat gctgcatcac gtggatcagc ggcagcgtcg
      361 gcttgccttc ggcgaggtcg tccccaaggt gcttgccggt gtcggcctcg tcggcggagt
      421 agtcgagcac gtcgtcgatg agctggaagg cggtgccaag gtgcatgcca aaggcggcca
      481 ggcgctgctc gagcgcgtcg tccgcaccgg ccacgatgcc gcccaggcgc gccgcggctt
      541 cgaagagctt ggcggtcttg tagcggatca cgcgcaggta gtcttcgatg acgacgtcgg
      601 cgttgtggca gttgagcagc tgcagcacct cgccctcggc gatgatgttg gtggcgtcgg
      661 cgagcacgcg catcacgcgc atctcgtcga ccccgaccat catctggaag gcacgcgaat
      721 agaggaagtc gcccaccagc acggcagagg cgttgccgaa ggcggcgttg gcggtacgct
      781 tgccgcggcg caggtcggac tcgtcgacga cgtcgtcgtg cagcaggctg gcggtgtgga
      841 tgaactcgac gaccgcggcg agctcgtggt gatgcacgcc gcgatagtcg agcgcgcccg
      901 ccgcgtagag caccagcgcg gggcgcagcc gcttgccacc gctgccgacg atgtattccg
      961 cgatgcggcg gatcagggcc acgtccgagt gcagtcggtt gcggatgacc gtatccaccg
     1021 cttgcatgtc ggcggcgatc ggcgcgaaga gctgctggac ggacaaggcg ttctggctcg
     1081 cggaaaatcg tagatgttag gcggcgcccg cgcgcggcgt caagccgcaa ccccgggcgc
     1141 cgcaggtgag cggtagcgag ctcagttgcc gttgcgggcg cgcgtcaccg cgttgtcgag
     1201 cgcttccttc gcggccttct cgtcggccca cacgccggcg agttcctcgt cgaggatgcg
     1261 gcgcaccttc tggcgctcga ccaccggctg cgccgacgac tgcggcgtgg cgggcttgcc
     1321 gccgagctgg tcgaccgcga cctggatgtt ctcgaggtcg ttgccgagca gctcgctgcg
     1381 cgaggccagc aggccggcgc ggttgagcgg caggtagccg gtctcgcgct gccacgcgac
     1441 ctggttgtcg ggctgcagcc agaaactcac gaagcgcgcc acggccttgt attcggccgg
     1501 tttcttgcct gccgaggccc acagtgcggg accgtcggcg atggtgttct gcggcgcacc
     1561 ggggaagtcg tcgtagtagg gcagcttgga caccgccaca tcgaccttgc cggcacggcg
     1621 gaagtcggtc cagctcgccg agggcgcggc gatcaccgcg cactcgccgc gcgcgaagcg
     1681 ctgctcggcc tcggcctgct ggtcgaacac gtgcaggtag cgcgcacgcg tccagctcgc
     1741 catcatcgcc acatgcttga cctgaaacat gccgttgaag ctcggtacgg tggtcttgcc
     1801 gctctgcgcg gccaccggct cgttatgcca ggccgagagg ttctccacca tcacgcggcc
     1861 gggctcggcc acggtatagg ggcaggtgtg accggtatcg gcgaggcgcc cgagggtttc
     1921 ctgcagatcg aaccaggtgt tgatccgggt ggtctccggg ttgaggccgg cctggcgcag
     1981 cgcgtcgcgg ttgaggaaca gcaccggggt cgacaggccg accggcagcg ccagcagctg
     2041 tcccttggca tcgaccgggg tgcgcgtcat catcgcgggc ggacgcaggg tctgcagcgg
     2101 cacgccactt tccttcatga gctggaagag cggcttgaag cgcggcttgc cggcgacgaa
     2161 ctcctcctcg tcgtcgccct cgaggatcat caggtggggc gcgcccgagc gccagtccgc
     2221 cgcggacatc acgacctgca cgtccttgga ctgcgcgttg aagcgctcga ccaaatcctt
     2281 cagcgccccc gccttggcat cgggcaggcg atggaagagc tggacctgct gctgggcgct
     2341 ggcctggacc accgagccgg gcgcgaagaa gagaccgccg gcagcgaccg ctgcggccaa
     2401 ggccttgaaa cgcatcttgt tcatcgattc gacacacaaa aaggagccgg gcaggacggc
     2461 ccggaagcgg gcgcgattat agatccgcgc gcactcgaat agtgggcctc gagggcgagc
     2521 gtgccgcgcg cgcgacacca ctcctctcaa gatggggtgg ccgcggccga taaccccacc
     2581 agaagaatgc cctcggatcg ccatgccccg cccgacccca tccccgacgc tgcgccgact
     2641 cgtcctcctc gccctgctgc ccttcgcagc cggctgcgac caactcgccg aggtgctcga
     2701 gctgcccaat ccatcgaagg tcgaggccga gggccgtgcg gtcgggagcg cctgccgcca
     2761 ctccgggcgc tcgctggagg actgctacgc gctcaatccg gcggcctcga aggcagcggt
     2821 gttcgccggc tggcgtgaca tgaacgacta catgatggag cacaagctcg agatcgtgcc
     2881 ctcgcagctc gtccagggcg cgccaaaggc agcgcctccc cggtccacgg cgcaggcggc
     2941 gccggcacag cccgcagcgc actgaccgcc gtacccgcac cgacgctcag ggcgccgggt
     3001 tgccgtgcag ctgatgaacc cgctcgatct cggcgagcgc atcctcgtcg agacgccgat
     3061 gccaggcgtc gaggttctcg gcgagttgcg ccaccgtggt cgcgcccacg atggtgctgc
     3121 ccacgaacca gcgcgaatac acgaaggcga gcgccagcgc ggtgaggctc atcccgcgcc
     3181 gccgcgcgat ctccgcgtag cgctccaccg ccgcacgcac accgggcttg gcgtagcgca
     3241 caccgaaatt ctcgaactcg gtgaggcgcc cgcgcgcagc cgggtcggcg agatacttgc
     3301 ccgacaggtg gccgaaggcg agcggcgaat acgccagcag cgccacgtcc tcgcgcagcg
     3361 tgatctcgct catgccgtac tcgtacaccc ggttgagcag gttgtaggcg ttctgcaccg
     3421 acaccacgcg tggcaggccg ctctcctgcg ccatgcgcac gaactccatc agcccccagg
     3481 gctgctcgtt cgacaggccg acgtggcgga tcttgccctc gtccaccagt tcggcgagcg
     3541 cctcgagctg ggcgcggatc ggcgtggatt cgcgctcgcg cgccggatcg aagggcagct
     3601 ggccgaacag cggctggttg cgcgccggcc agtgcagctg atacaggtcg atgtagtcgg
     3661 tgcgcagccg gcgcaggctg ccctcgaccg cggtgcggat gttggcgcgg tcgagcgcga
     3721 gcggaccgcc gcggatccac tccaggctgc gcgccggccc cgccaccttg gtcgcgatca
     3781 ccagcttctc gcgcacctgg cgcgccagcc agttgccgac gatgcgttcg gtctcgccgc
     3841 tggtctcggc acgcgccggg accggataca tctcggcggt gtcgatgaag tcgatcccgc
     3901 gctccacggc gaggtcgagc tggctgtgcg cctcggcctc gctgttctgc tggccaaagg
     3961 tcatggtgcc caggcacacc gccgacacct gcggccccgc tccgcccagc cggcggtatt
     4021 ccatttcacg tacagccatc ccgtatcccc ttttccgaca accgaatcgt gacctgcctc
     4081 gctcctgcgg gctgctccgg aggcgcgagg gtgtgccttg ggagcgacgc gaatcgcaac
     4141 tgcggcgcgc ggaacccgcg caggcgcggc ggccggcccg atctcaacct gcgtcgtagc
     4201 gccggcatgc tcgccagcgc ggcgctcgcg gcttatcatc cgctgccttg cgccgattcc
     4261 ggttgccgcc cctgcggccc ggcacggacc catcatactg cagcccctgc gcgcaccgac
     4321 cccatgaacc agcacacccc cgccgccgcg ctcgcctgcg ccggcctcct cggctattgc
     4381 cgcgccggct tcgagaagga actcgccgcc gaactcgacg acatcgccgc cgaagccggc
     4441 ctgatcggct acgtgcgcgc cgagcccgac tcgggctacg tgatctacga gaccttcgag
     4501 cccaccccgc tcggcagctt cggcgaatcc accgactggc gccgcccggt gttcgcgcgc
     4561 cagctcctgc cgtggttcgc gcgcgtcgac gatctgcccg agcgcgaccg cgccacaccg
     4621 atcgtcgagg cggtgaaggc cagcggccag cgcttctccg gggtgatgct ggagactccg
     4681 gacaccgacg aggccaagca gcgctcgggc ttctgcaagc gcttcaccga gccgctcgcc
     4741 aaggcgctcg agaaggccgg ctgcctgcgc agcagccgcg ccggcctgcc ggtcctgcac
     4801 gtgctcttca ccagcgccac caccgcctgg ctcgccgccg gccagcccgg gcagtgctcg
     4861 acctggccga tgggcatccc gcgcctgcgc atgccctcga acgcgcccag ccgctccacc
     4921 gccaagctgt ccgaagcctt catgatgctg ctcgaggaag gcgagcgcga cagcatcctg
     4981 cgcgccggcc agcgcgcggt cgatctcggc gccgcgccgg gcgggtggac ctggcagctc
     5041 gtgaatcgcg gcctgcgcgt caccgcgatc gacaacggcc cgctgcgcga cagcgtgatg
     5101 gccaccgaga tggtggagca cctcaaggcc gacggcttca cctggcgccc gcaccggccg
     5161 gtggactgga tggtgtgcga catggtcgag cagccctcgc ggatcgcctc gctgatggcc
     5221 gaatgggtcg ccaccgggcg ctgccgctac acgatcttca acctcaagct gccgatgaag
     5281 cgccgcgtcg aggcggtcga gcagtgccgc gagctgatcc gcaagcggct cgccagcgtc
     5341 ggcccctacg acctgcgcat caagcacctc taccacgacc gcgaggaggt caccgccttc
     5401 ctcacgctca agcgctgagc ggaccaggcg cggcggaccg ccgccgcgcg gtcggcggcg
     5461 aagcccctgt ccggtaacgc tttttgtgcc gtgtataatc cgggttttcc gtcatccggc
     5521 ggtcgactgt cgaccgtctc caacccgaag gcacccatga gctttgccga tctcggcctc
     5581 attcccgaac tgctgcaggc cgtcaccgac gccggctaca ccgagcccac gccgatccag
     5641 cgccaggcca tccccatcgt catcgccggc aaggacgtca tgggcggcgc ccagacgggc
     5701 accggcaaga ccgccggctt caccctgccg ctgctgcacc gcatcgcccg ccacgccaac
     5761 accagcacct cgcccgcccg tcaccagacg cgcgcgctga tcctcgcgcc cacgcgcgag
     5821 ctcgcgatgc aggtgttcga gtcggtcaag acctacagca agcacctgcc gctgcgctcg
     5881 gtgtgcgtct atggcggcgt cgatatccgc ccgcagcagg ccgagctgcg ccgtggcatc
     5941 gaggtcgtga tcgccacgcc gggccggctg ctcgaccacc tcgagcagaa gtcgatcaac
     6001 ctgtcgcagg tcgaggtgct ggtgctggac gaagccgacc gcatgctcga catgggcttc
     6061 atccccgaca tcaagcgcat cctcgccctg ctgcccaagc agcgccagag cctgctgttc
     6121 tcggccacct tctcggacga gatcaagcgc ctcgccgacc agatgctgaa ggacccgcag
     6181 ctgatcgagg tggcgcgccg caacatggtc tccgagacca tcacccacgt ggtgcacccg
     6241 gtgtcctcgg gcatgaagcg caacctgctc gcccacctgc tgcgccacaa gcccgacact
     6301 caggccctgg tcttcgtcga caccaagctc atgtgcggcc gcttggcgca ctacctcgag
     6361 cgcagcggca tctcggccga cgccatccac ggcgacaagg gccagcagca gcgcaccgac
     6421 acgctcgagg ccttcaagtc gggcaagctg cgcgtgctgg tcgccaccga cgtggccgcg
     6481 cgcggcctcg acatcgacga gttgcccttc gtgatcaact tcgagctgcc gcacaccgcc
     6541 gaggactacg tgcaccgcat cggtcgcacc ggccgcgccg gccaccacgg ctatgcgatc
     6601 tcgctggtca gctcggaaga aaagcactgg ctgtccgaga tcgaaaagct gatcaagctg
     6661 cagattccgc aggagagggt acccggcttc gaccccgatc ccgacttcca cgaggccggc
     6721 agccgcggcc atcgtggccg caggccggct gccgcggccg ccgacaccga ggcgcaggct
     6781 ccggcggcgc gctccggctg gtgagcgagg cgggagagcg cctcgtgggc atc
//
LOCUS       DAOQNN010000153         6752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6202_length_6752_cov_9.213230, whole genome shotgun sequence.
ACCESSION   DAOQNN010000153 DAOQNN010000000
VERSION     DAOQNN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6202_length_6752_cov_9.213230"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="PKV97_09420"
     CDS             <1..160
                     /locus_tag="PKV97_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNB06133.1"
                     /translation="FAMVHAASHEAALARVEEIAALIAAEFPGACRNRDVLFSSGILK
                     KTGLRIGA"
     gene            274..1482
                     /gene="nirJ"
                     /locus_tag="PKV97_09425"
     CDS             274..1482
                     /gene="nirJ"
                     /locus_tag="PKV97_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HNB06134.1"
                     /translation="MFRISQFIRELDHPTPAGPRRNPPGPVVIWNLIRRCNLTCEHCY
                     SISADKDFPGELNTQEVFKVMDDLKDARVPVLILSGGEPLLRPDIFDIARRAKGMGFY
                     VGLSSNGTLIDESNIDAIDEIGFDYVGVSLDGIGATHDHFRRKQGAFEASMHGIRLCR
                     ERGIKVGVRYTMTEGNAHDLPALLKLVEDEAIDKFYFSHLNYAGRGNKNRAGDARHRT
                     TREAMELLFETCLDLHRRGIDKDFVTGNNDADGPFLLHWVQRRFPDKAAHIEARLRQW
                     GGNASGVNVANIDNLGILHPDTMWWHVPLGNVRQRAFGEIWNDLSNPLLAGLKQHPRK
                     LEGRCGACKYLEICNGSSRVRAEQVTGNPWAEDPACYLYDEEIGVNPADYGAERVVTT
                     PWSRIDKVSA"
     gene            1479..>3033
                     /locus_tag="PKV97_09430"
     CDS             1479..>3033
                     /locus_tag="PKV97_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNB06135.1"
                     /translation="MKIRASTLTGLFALAGALVVLPAAASPDKAVARAASGAAASPAA
                     IDVPALYAQHCAACHAPNRLGAMGPALLPDNLGRLRKSEAAKVIGEGRPATQMAGFAA
                     QLGKDEIAALTEWIYTPVVPEPRWTDEDIRASRVVHVDTATLSDKPVFDADPLNLFLV
                     VEAGDHHVSVLDGDRLEPIHRFQSRFALHGGPKFAANGRYVFFASRDGWITKFDLWNL
                     KVVAEVRAGINTRNVAASPDGTHVAVANYLPNTLVLLDGELNLVKTIPAMDRDGKKSS
                     RVSAVYDATPRQSFIAALKDVGEVWEISYTKAVEDIPVSYIHDYTQREGSFIPGYLNP
                     RRTILAEVLDDFFFTQDYSELMGASREGVGQVVNLDVRKKIANLPLDGMPHLGSGITW
                     EWQDPAAGPGARPRTVMASTNLKAGEVTVIDMKTWEVVKRIPTRGPGFFLRSHSSSRY
                     AFVDSMMSAEAKHILQVIDKQTLEVVREITGEPGKTLAHVEFTRDGRHALASLWEDEG
                     AVIVYDARTL"
     assembly_gap    3034..3133
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3357..4544
                     /locus_tag="PKV97_09435"
     CDS             3357..4544
                     /locus_tag="PKV97_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNB06136.1"
                     /translation="MALIRLDEPTTKRIPPDTFSLWALAFRPFYLLAALFAAIAVPVW
                     AVAYSGAIDLPMPGIWWHSHEMIFGFAVAVIIGFLFTAGRNWTGLDTPTGKPLMVLAA
                     VWLAGRLAMAFGSGAWVAIVDLAFLPVAAGMLLKVLIKAKSKRNYFVGALPAMLALAN
                     LFFHLAVLGVIEADPLTAMHLALGLVVVLETIVGGRVIPMFIFNAVRGVKQWRNVKVD
                     WAAAIATGIALLLWALGAGGWAGVVSLIAAVLQAVRLGGWNPWATRGHPILWVLQLAY
                     LWIPLGLALLALTQFGVLPRSAGVHALAIGATGGLIIGMITRTALGHTGRMLIAGRIE
                     TAAYALVLIAALVRVLTVGFFPAAQFGGVHAAATLWSIAFLLYLWRYAPYLWYARVDG
                     KEG"
     gene            complement(4709..5470)
                     /locus_tag="PKV97_09440"
     CDS             complement(4709..5470)
                     /locus_tag="PKV97_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB06137.1"
                     /translation="MPRNATPVGERPVRIDSSPASPVTEIDRLCRLLARAPLFNGLAC
                     EDIVRFARGVREVRVARGEILFHRGDPCHGFHLILDGQIKLAFTSAEGNEKVVDILYA
                     GRSFGEAVMFMEKPYVVMAQALTDATLLHIGKQIFFEEMSGDPLFCRKIIAGLAQRLH
                     LLMADVESYSLRSGRDRVVGYLLREEERDGDGVAEGSVSLRLPTSKGTIASRLNLTQE
                     HFSRILSELTSAGLIQVEGRTIHIPDIGRLRRSLG"
     gene            complement(5475..>6752)
                     /locus_tag="PKV97_09445"
     CDS             complement(5475..>6752)
                     /locus_tag="PKV97_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HNB06138.1"
                     /translation="TQPVLLWLSDFRASAASATGGPGTGGELGEAGARRPGKDSSRQA
                     HRVERQDKMPEKHGMIIPFRAESLLSVAEFIKVQRSTDDEPDDNAADAAANLDHLSIT
                     RDGERVASKVRFDLDLPSAAEDDVVLGDGIPLPEWDYRKNLLLEDHVRLAELTPSIHD
                     PRAAPCALPEHLRRTARRLHRQFAALTPGRRWLKAQVDGTELDLDAVVRAATDRATGH
                     HPSDQLYLSLEKRERDLACLALADLSLSTDSWVSSEARVIDVIRDSLLLFGEALLATG
                     DSFALCGFSSVKRSNVRFHRLKDFDQRFDDRARGRIMAIKPGYYTRLGAAIRHATTIL
                     DRQRAARRILLILSDGKPNDLDLYDGRYGIEDTRVAVVEARNRGVVPFCVTIDREGAS
                     YLPHLFGPAGYAVIRQPDELPARLPMFYAQLTR"
BASE COUNT         1045 a         2232 c         2308 g         1067 t
ORIGIN      
        1 gttcgcgatg gtgcacgccg ccagccacga ggccgcgctc gcgcgcgtgg aagagatcgc
       61 cgcgctgatc gcggcggagt ttcctggtgc ctgccgcaac cgcgacgtgc tgttctcgtc
      121 cggcatcctg aaaaagacgg gcctgcgcat cggcgcctga tccggcaagc gactcttccg
      181 gccggcacga gcgcccgccc cgggcaccct tgacgcgcat cattttcatt cgcgggcgcc
      241 tcgggtgaaa tggccagcat tcaggaggct cccatgttcc gcatctccca attcatccgc
      301 gagctcgacc accccacgcc tgccggcccc cgccgcaacc cgccaggtcc ggtggtgatc
      361 tggaacctga tccggcgctg caacctcacc tgcgagcact gctactcgat ctcggcggac
      421 aaggatttcc ccggcgagct gaacacacag gaagtcttca aggtcatgga cgacctcaag
      481 gacgcacgcg tgccggtgct gatcctgtcc ggtggcgagc cgctgctgcg ccccgacatc
      541 ttcgacatcg cccgccgcgc caagggtatg ggcttctacg tcggcctgtc gtccaacggc
      601 accctgatcg acgaatccaa cattgatgcg atcgacgaga tcggcttcga ctacgtcggc
      661 gtcagcctcg acggcatcgg cgccacgcac gaccacttcc gccgcaagca gggcgccttc
      721 gaggcctcga tgcacggcat ccgcctgtgc cgcgagcgcg gcatcaaggt cggcgtgcgc
      781 tacacgatga ccgagggcaa cgcccacgac ctgcccgcgc tgttgaagct ggtcgaggac
      841 gaggccatcg acaagttcta cttctcgcac ctgaactacg ccgggcgcgg caacaagaac
      901 cgcgccggcg acgcccgcca ccgcaccacc cgcgaggcca tggagctgct tttcgagacc
      961 tgtctcgacc tgcaccggcg tggcatcgac aaggacttcg tcaccggcaa caacgatgcc
     1021 gacggcccct tcctgctgca ctgggtgcag cgccgcttcc ccgacaaggc cgcccacatc
     1081 gaggccaggc tgcgccagtg gggcggcaac gccagcgggg tgaacgtcgc caacatcgac
     1141 aacctgggca tcctgcaccc ggacaccatg tggtggcacg tgccgcttgg caacgtgcgc
     1201 cagcgcgcct tcggcgagat ctggaacgac ctgtcgaacc cgctgctcgc cggcctcaag
     1261 cagcatccgc gcaagctgga ggggcgctgc ggcgcttgca agtacctgga gatctgcaac
     1321 ggcagctcgc gcgtgcgcgc ggagcaggtc accggcaacc cgtgggcgga ggacccggcc
     1381 tgctacctct acgacgagga gatcggcgtg aatccggcag actacggcgc cgagcgcgtc
     1441 gtcacgacgc cctggagccg catcgacaag gtttccgcat gaagatccgc gcctcgaccc
     1501 tgaccggcct gttcgcgctc gccggtgcac tggtcgtctt gcctgccgcc gcttcgccgg
     1561 acaaggccgt cgctcgggcc gcgtccgggg cagcggcgtc gccggctgcc atcgacgtac
     1621 ccgcgctcta cgcgcagcat tgcgcggcct gccatgcgcc caatcgcctc ggagcgatgg
     1681 ggcccgcgtt gctgcccgac aacctgggcc gcctgcgcaa gagcgaggct gccaaggtga
     1741 tcggcgaggg ccggcccgcg acccagatgg ccggcttcgc cgcgcagctg ggcaaggacg
     1801 agatcgccgc gctcaccgag tggatctaca ccccggtggt gcccgagccg cgctggaccg
     1861 acgaggacat ccgcgcctcg cgcgtcgtcc atgtcgacac cgcgaccctt tccgacaagc
     1921 cggtgttcga cgccgacccg ctcaacctct tcctggtggt ggaggcgggc gaccaccacg
     1981 tcagcgtgct cgacggcgac aggctcgagc cgatccaccg cttccagtcg cgcttcgcgc
     2041 tgcatggcgg gcccaagttc gccgccaacg ggcgctacgt gttcttcgcc tcgcgcgacg
     2101 gctggatcac caagttcgat ctgtggaacc tcaaggtcgt ggccgaggtg cgtgccggca
     2161 tcaacacacg caacgtcgcc gcatcgcccg acggcaccca cgtcgcggtg gccaactacc
     2221 tccccaacac gctggtgctg ctcgacggcg agctgaacct ggtcaagacc atcccggcga
     2281 tggaccgcga cggcaagaag agctcgcggg tgtcggcggt gtacgacgcc accccgcgcc
     2341 agtccttcat cgccgcgctc aaggacgtcg gcgaggtgtg ggagatcagc tacaccaagg
     2401 cggtcgagga catcccggtc agctacatcc acgactacac acagcgcgaa ggcagcttca
     2461 ttcccggtta cctcaacccg cgccgcacca tcctcgccga ggtgctcgac gacttcttct
     2521 tcacccagga ctattccgag ctgatggggg cctcgcgcga gggggtcggc caggtggtga
     2581 acctcgacgt gcgcaagaag atcgccaatc tgccgcttga cggcatgccg cacctgggct
     2641 cgggcatcac ctgggaatgg caggaccccg ccgccggtcc gggcgccagg ccgcgcacgg
     2701 tgatggcgag caccaacctc aaggcgggcg aggtcaccgt gatcgacatg aagacctggg
     2761 aggtggtgaa gcgcatcccc acccgcggcc cgggcttctt cctgcgcagc cactccagca
     2821 gccgctatgc gttcgtcgat tcgatgatga gcgcggaggc caagcacatc ctgcaggtca
     2881 tcgacaagca gacgctggag gtggtgcgcg agatcaccgg tgagccgggc aagacgctcg
     2941 cccacgtcga gttcacccgc gatggccgcc atgcgctcgc cagcctgtgg gaggacgagg
     3001 gcgcggtgat cgtctatgac gcgcggaccc tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnngccagcc tgtgggagga cgagggcgcg gtgatcgtct atgacgcgcg
     3181 gaccctcgag gaggtcaagc gcctgccgat gagaaagcct gtgggcaagt acaacgtatg
     3241 gaacaagatc acgcgcgagg aaggcaccag ccactgattc ctcccggccg ggcaggtgtg
     3301 cggccgtgcg cagcttcatc cgccttcaac ccgttcagca gaaaagagaa ccgaacatgg
     3361 cgctgatccg cctcgacgaa cccaccacca aacgcattcc gcccgacacc ttctcgctgt
     3421 gggcgctcgc ctttcgtccc ttctacctgc tcgccgcact gttcgcagcg atcgcggtgc
     3481 cggtttgggc ggtggcctac tcgggcgcga tcgacctgcc catgccggga atctggtggc
     3541 attcgcatga gatgatcttc ggcttcgccg tggcggtgat catcggcttc ctgttcaccg
     3601 cagggcgcaa ctggaccggg ctcgacaccc cgacgggcaa gccgctcatg gtgctcgccg
     3661 cggtgtggct cgccgggcgc ctggcgatgg ccttcggcag cggagcctgg gtggccatcg
     3721 tcgatctggc cttccttccg gtcgcggcgg ggatgctgct gaaggtgctg atcaaggcca
     3781 agagcaagcg caactacttc gtcggtgcgc tgcccgcgat gctcgcgctc gcgaacctgt
     3841 tcttccacct cgccgtgctc ggcgtgatcg aggccgatcc gctgaccgcc atgcacctgg
     3901 ccctcggcct ggtggtggtg ctggagacca tcgtcggcgg ccgcgtgatc ccgatgttca
     3961 tcttcaacgc ggtacgtggc gtcaagcagt ggcgcaacgt gaaggtcgac tgggcggcgg
     4021 cgatcgccac cggtatcgcg ctgctgctgt gggcgctggg tgccggaggc tgggcgggtg
     4081 tggtgtcgtt gatcgcagcc gttctgcagg cggtgcggct aggcggctgg aacccttggg
     4141 cgacgcgcgg ccatcccatc ctgtgggtgc tgcaactggc ctatctgtgg attccgctcg
     4201 gcctggcgct gctcgcgctt acccagttcg gcgtgctgcc gcgctcggcc ggagtccatg
     4261 ccctggccat cggcgcgacc ggcggcctga tcatcggcat gattacccgc accgcgctcg
     4321 gccacaccgg ccgcatgctg atcgccggtc gcatcgagac cgcggcctac gccctggtgc
     4381 tgatcgccgc gctggtgcgg gtgctgaccg tgggcttctt cccggccgca cagttcggcg
     4441 gcgtgcatgc ggccgccacg ctgtggtcga tcgccttcct gctctacctg tggcgctacg
     4501 ccccctacct ctggtatgcg cgggtggacg gcaaggaggg ttgagcgctt gcggggcgcc
     4561 cacaaacagg aaaggggcct gcgggcccct tttgcatcgc ccggacattc acgacggctc
     4621 aagaatcgcc ctgcaggagc gatgcgagac gcgattgcga cgttccggaa gcgtgggtgc
     4681 gggtttgacg cgctgaaagg cgtgaccctc aacccaggct tcggcgcagg cggccgatgt
     4741 cggggatgtg gatggtgcgg ccttcgacct ggatgaggcc tgcgctggtg agttcgctga
     4801 ggatgcggga gaagtgttcc tgggtgaggt tgagccgcga ggcgatcgtg cccttgctgg
     4861 tgggcaggcg caacgacacc gagccttcgg ccaccccgtc gccgtcacgc tcctcttcgc
     4921 gcagcagata gcccaccacg cggtcgcgac ccgaacgcag cgagtagctc tcgacgtcgg
     4981 ccatcagcag atgcagtcgc tgggccaggc cggcgatgat cttgcggcag aacaagggat
     5041 cgcccgacat ttcctcgaag aagatctgct tgccgatgtg cagcagggtg gcgtcggtca
     5101 gtgcctgcgc catcacgacg tagggctttt ccatgaacat caccgcctcg ccgaagctgc
     5161 gtccggcgta gaggatgtcg accaccttct cgttgccctc ggccgacgtg aaggcgagct
     5221 tgatctggcc gtcgaggatg aggtggaagc cgtggcaggg atcgccgcga tggaacagga
     5281 tctcgccgcg cgcgacccgt acctcgcgca caccacgcgc gaagcggacg atatcctcgc
     5341 aggcgaggcc gttgaaaagc ggcgcgcgcg cgagcaaccg gcacaggcgg tcgatctccg
     5401 tgaccgggct ggcgggggag gaatcgatcc ggacaggtct ttccccgacc ggggtggcgt
     5461 tacgaggcat ggcttcagcg ggtgagctgg gcatagaaca tgggcaggcg tgcgggcaac
     5521 tcgtcgggtt ggcggatcac cgcgtagccg gccggaccga agaggtgcgg caggtagctt
     5581 gcgccttcgc ggtcgatggt aacgcagaac ggcaccacac cgcggttgcg tgcctcgacg
     5641 acggcgacgc gggtgtcctc gatgccgtag cggccgtcgt agagatcgag gtcgttgggt
     5701 ttgccgtcgg acaggatcag caggatgcgg cgtgcggcgc gctggcggtc gaggatcgtg
     5761 gtggcgtggc ggatcgccgc gcccaggcgg gtgtagtagc ccggcttgat cgccatgatg
     5821 cggccgcgcg cgcggtcgtc gaagcgctgg tcgaagtcct tgaggcggtg gaagcgcacg
     5881 ttgctgcgct tgaccgagga gaagccgcat agcgcgaagc tgtcgccggt ggcgagcagg
     5941 gcttcgccga agagcagcag cgagtcgcgg atgacgtcga tcacacgcgc ctcggaggag
     6001 acccaggaat cggtcgacag cgacagatcg gccagcgcca ggcaggcgag gtcgcgctcg
     6061 cgcttctcga gcgagaggta gagctggtcg gacggatggt ggccggtggc gcggtcggtg
     6121 gcggcgcgca ccaccgcgtc gaggtcgagc tcggtgccgt cgacctgcgc cttgagccag
     6181 cgtctgcccg gagtgagcgc ggcgaactgg cggtgcagcc ggcgcgcagt gcggcgcagg
     6241 tgctcgggca gggcgcaggg ggcggcgcgc gggtcgtgga tcgagggcgt gagttcggcc
     6301 aggcggacgt ggtcctcgag cagcaggttc ttgcggtagt cccactcggg cagcgggatg
     6361 ccgtcgccga gcacgacgtc gtcctcggca gccgagggca ggtcgaggtc gaagcgcacc
     6421 ttggaggcga cgcgctcgcc gtcgcgggtg atcgacaggt ggtcgaggtt ggcggcggcg
     6481 tcggcggcgt tgtcgtcggg ctcgtcgtcg gtgctgcgct ggaccttgat gaactccgcc
     6541 accgacagca ggctctcggc gcggaagggg atgatcatgc cgtgcttctc gggcatcttg
     6601 tcctggcgct cgacgcggtg ggcctggcgg ctgctgtcct tgccgggacg gcgggcgccg
     6661 gcctcgccga gctcgccgcc cgtgccaggg ccgccggtgg cactcgcggc gctggcgcgg
     6721 aagtcgctca gccacagcag cacgggctgg gt
//
LOCUS       DAOQNN010000154         6735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6238_length_6735_cov_8.713473, whole genome shotgun sequence.
ACCESSION   DAOQNN010000154 DAOQNN010000000
VERSION     DAOQNN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6238_length_6735_cov_8.713473"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PKV97_09450"
     CDS             complement(<1..953)
                     /locus_tag="PKV97_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB06139.1"
                     /translation="MAADGIVILGAGPAGAAVAIGLARMGEPVVLVGEPRRFAAVEGV
                     SARVIDALRGLGLQRALTAFAPPSPRRAVWNGTYTEANVESLVDRQRFDQGLLEDLER
                     LGVPVVRGRVTALHPAPGGHELEIDTDAGPRRMAAGFLVEARGRAAPGGGAPRVRGVE
                     TVSLLQYWQGPAGEAGSAVLSVEDGWMWMAALADGRRYLQLTVDVASADLPPKRALGD
                     YCSARFHAVEAAAPFVRDAQPVGEPHARTSTAVLNESVAGDDWIRVGDAAMAVDPLSG
                     NGIFQALSSALQAPAVVATLRHDRGRTALAQHFHTRRIEHL"
     gene            complement(943..1626)
                     /locus_tag="PKV97_09455"
     CDS             complement(943..1626)
                     /locus_tag="PKV97_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB06140.1"
                     /translation="MTVRIAVVDSGCTPAQALQVRDAVALVLEDRAVRQAPATADALG
                     HGGRVAGILLHCAPQAELLVVQVFRERLTTTAAQVAAAIDWAVANGAQLVNLSLGLRE
                     PRPVLAEACARALAAGTVLCAAAPARGQTVYPAGFPGVLRVTGDARCAPGELAAIGSA
                     EIDFGAHVRPLDGSLAGAGASMACAWFTGLAARHFARGGDADALRAWLVEQARHHGVD
                     DPRRRQHGG"
     gene            complement(1623..3359)
                     /locus_tag="PKV97_09460"
     CDS             complement(1623..3359)
                     /locus_tag="PKV97_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB06141.1"
                     /translation="MLAGLIHAERPDELRRGLRWLYGFVRPQRRRIAGLLLLSCAATL
                     LVLLQPWLTKLLIDEGLLARDYRTLLMVAGAMIAVGIVGTVLAGVNRQLHTRLSGSIL
                     FALRSDLYRHLQTLSPAFYGRQRMGDLLSRLDGDVAEIQRFAVDSLFAAVSSVIGLLG
                     AVALMLSLSWKLSLLVLVLVPLETLWLRRMRRKVEARTRTVRERAADVSSFLVETLPA
                     MKFIQASRREADERSRLDDLGARYLDDLLRLQRTEFLTHAIPSTLTSATRAAAFMIGG
                     WWVIEGSWQLGALIAFSTYLGMATGPVNSLLGLYVAIQRMSVSLRRVSELREAPADVV
                     DSAHPGAAQRAVPAWQGRIEFDAVVFHHQGRAEAVLDGARLTVPAGSKVALSGASGVG
                     KSTLIDLLHRHYDPQAGTIRLDGADLRALPLGALRRAVAVVSQDIVLFRGTLAENIRY
                     AAPQADEGAVRAAARRAHLDALIASLPQGLETALGERGQQLSGGQRQRIAIARALLQE
                     PCVLVLDEATSAVDEATEAAIIAEVDALFAGRTRILISHRAATLAGCDLRVHLERGRL
                     EVLPPAAVTVRS"
     gene            complement(3454..4374)
                     /locus_tag="PKV97_09465"
     CDS             complement(3454..4374)
                     /locus_tag="PKV97_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB06142.1"
                     /translation="MSARPHLPASLQVFERGWLSANNVLLFDGDEATLIDSGYVSHAD
                     QTVRLVRAALDGRSLGRLINTHSHSDHIGGNASVQRAFGCAITVPEGMARAVEEWDEE
                     ALLLSVGAQAGERFRHDATLAAGARFVAGELEWQAVAAPGHDMDALAYYNPDRRILIS
                     GDALWRDGFGILFAEVMGQGDALGEARRTLEAIGRLAVDAVIPGHGAPFAEFDDALER
                     AFARLRAFEDDGARMARNAIRACITFKLLEVRSMALVELPRHLDETPLYRTANARFLG
                     LDADALAAWLVKELERAGVARREHGMLVAS"
     gene            complement(4371..4592)
                     /locus_tag="PKV97_09470"
     CDS             complement(4371..4592)
                     /locus_tag="PKV97_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNB06143.1"
                     /translation="MSGDALCVGVCMIDWDSGVCLGCGRTADEINGVPTPAPGAQVAG
                     QPDAAASPAQAAGVPLPPNVAARVGEGSD"
     gene            complement(4589..4789)
                     /locus_tag="PKV97_09475"
     CDS             complement(4589..4789)
                     /locus_tag="PKV97_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNB06144.1"
                     /translation="MSVPSPCINLCRMSPDTGWCEGCQRTIEEITRWSRSTDDDRRAI
                     LAAVAERRTHLAAASPAVDLRT"
     gene            complement(4951..5886)
                     /locus_tag="PKV97_09480"
     CDS             complement(4951..5886)
                     /locus_tag="PKV97_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNB06145.1"
                     /translation="MANSILPAAVRIVEMGPRDGLQNEKQLVSTDTKLELVRRLERAG
                     LKAIETTSFVSPKWVPQMGDNAELLPRVLAAAAPGVAYPVLTPNLKGFEAALAAGARE
                     VAVFGAASESFSQKNINCSIAESLERFRPVTDAAHAAGVRVRGYVSCVVGCPYEGAVA
                     PQAVADVAATLLEMGCYEVSLGDTIGVGTPAGIRRMLEAVAKRVPVDKLAGHYHDTYG
                     MAVANVFASLEMGVAVFDASVGGLGGCPYAAGASGNVATEDVVWLLNGLGIDTGIDLD
                     SLVDTAAWISAQLGREPASRVARAVLAKRAKTACA"
     gene            complement(5996..>6735)
                     /locus_tag="PKV97_09485"
     CDS             complement(5996..>6735)
                     /locus_tag="PKV97_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB06146.1"
                     /translation="SLRTGIMAGSPCPIEVMKRVIGKMNMSEVTIAYGMTETSPVSFQ
                     SGTDDPIDRRVSTVGRIQPHLEVKIVDNEGRIVPRGQSGELCTRGYSVMLGYWDDEAR
                     TKEAIDAGGWMHTGDLAVIDDEGYCNIVGRIKDMVIRGGENIYPREIEEFLYAHPKVL
                     DVQVVGIPDQKFGEELCAWIIPREGERLTEDEVREYCKGQIAHYKIPRYIRFVDSFPM
                     TVTGKIQKFLIRQRMKEELGLDEAKTA"
BASE COUNT          998 a         2486 c         2355 g          896 t
ORIGIN      
        1 aacaggtgct cgatacgccg cgtatggaag tgctgcgcga gcgcggtgcg gccccgatcg
       61 tgacggaggg tagccaccac cgccggtgcc tgcagcgcgg acgacagcgc ctggaagata
      121 ccgttgcccg agagaggatc gaccgccatc gccgcatcgc cgacgcggat ccagtcgtcg
      181 ccggccaccg attcgttgag caccgccgtg ctggtgcgcg cgtgcggttc gccgaccggc
      241 tgcgcgtcgc ggacgaaggg cgccgccgcc tcgaccgcgt gaaaccgtgc gctgcagtag
      301 tcgcccaggg cccttttcgg cggcaggtcg gcgctggcaa cgtccaccgt cagctgcaga
      361 tagcgccgtc catcggccaa cgcggccatc cacatccagc catcctcgac gctgagaacg
      421 gccgagcccg cctcgcccgc cggtccctgc cagtactgca gcagcgacac cgtctccact
      481 ccgcgcaccc gcggcgcccc cccgccgggc gcggcacggc cgcgcgcctc gacgaggaag
      541 cccgccgcca tgcggcgcgg cccggcatcg gtgtcgatct ccagctcatg gccccctggc
      601 gcggggtgca gcgccgtcac ccggccgcgc accacgggca cgccgaggcg ctccaagtcc
      661 tcgagcagcc cctgatcgaa gcgctggcgg tcgaccaggc tctccacgtt cgcctcggtg
      721 taggtgccat tccacaccgc ccgccgcggc gaaggcggcg cgaacgccgt cagcgcccgc
      781 tgcaggccga ggccgcgcag cgcgtcgatg acgcgcgccg agacgccctc gaccgcggcg
      841 aaacggcgcg gctcgcccac cagcaccacc ggctcgccca tccgcgccag tccgatcgcc
      901 accgccgcgc ccgcggggcc ggcgccgagg atgacgatgc cgtcagccgc catgctgtct
      961 tctcctcggg tcgtccacac cgtggtggcg cgcctgctcg accagccagg cacgcagtgc
     1021 gtccgcgtcg ccgccccgcg cgaagtgccg cgcggccagt ccggtgaacc aggcacaggc
     1081 catgctcgca cccgcgccgg cgaggctgcc gtcgagcggc cgcacatggg caccgaaatc
     1141 gatctctgcg gagccgatcg cggcgagctc accgggcgcg cagcgcgcat ctccggtcac
     1201 ccgcagcacg cccggaaaac ccgccggata cacggtctgg ccgcgcgcgg gcgccgccgc
     1261 gcacagcacg gtgccggccg ccagtgcgcg cgcgcaggcc tcggccagca ccggccgcgg
     1321 ttcgcgcagg cccagactca gattgacgag ctgggcgccg ttcgccaccg cccagtcgat
     1381 cgccgccgcc acctgcgccg cggtggtcgt gaggcgctcg cggaacacct ggacgaccag
     1441 cagctcggcc tgcggcgcgc aatgcagcag gatcccggcc acgcgaccgc cgtggccgag
     1501 cgcgtcggcg gtggccggcg cctggcgcac ggcacggtct tcgagcacca gggcgaccgc
     1561 gtcccgcacc tgcagcgcct gtgctggcgt gcacccgcta tcgaccacgg cgatccgcac
     1621 cgtcatgacc gcaccgtcac cgccgccggc ggcagcacct cgagccgccc gcgctcgagg
     1681 tgcacgcgca gatcgcaacc ggcgagcgtc gccgcgcggt ggctgatcag gatccgcgtg
     1741 cgtccggcga agagcgcatc gacctcggcg atgatcgccg cctcggtcgc ctcgtcgacc
     1801 gccgaggtcg cctcgtcgag caccaggacg cacggctcct gcagcagcgc acgcgcgatc
     1861 gcgatgcgct ggcgctggcc gccggagagc tgctgaccgc gctcgccgag cgctgtctcc
     1921 agcccctgtg gcaggcttgc gatcaaggcg tcgaggtgcg cccgccgcgc cgcggcacgc
     1981 accgccccct cgtccgcctg cggcgcggcg tagcggatgt tctcggcgag cgtgccgcgg
     2041 aacagcacga tgtcctggct caccaccgcc accgcgcggc gcagcgcgcc gagcggcagc
     2101 gcgcgcaggt cggcgccgtc gagccggatg gtcccggcct gggggtcgta atgacggtgc
     2161 agcaggtcga tcagcgtgga cttgccgacg cccgaagcgc cgctcagtgc gaccttgctg
     2221 cccgcgggta ccgtcagtcg ggcgccgtcg agcaccgcct cggcgcgtcc ctggtggtgg
     2281 aagacgaccg catcgaactc gatccggccc tgccacgccg gcacggcccg ctgtgccgcg
     2341 ccggggtgcg cgctatccac gacgtcggcc ggcgcctcgc gcagctcgct cacccgccgc
     2401 agactcacgc tcatgcgctg gatcgcgaca tacaggccga gcaggctgtt gaccggcccg
     2461 gtggccatgc cgaggtaggt ggagaacgcg atcagcgcgc cgagctgcca gctgccctcg
     2521 atcacccacc agccgccgat catgaacgcg gcggcgcgcg tcgccgaggt cagcgtggac
     2581 gggatcgcat gggtgaggaa ctcggtgcgc tgcaggcgca gcagatcgtc gaggtagcgc
     2641 gcgccgagat cgtcgagccg gctgcgctcg tcggcctcgc ggcgcgaagc ctggatgaac
     2701 ttcatcgccg gcagggtctc gaccagaaag ctcgagacat ccgccgcgcg ctcgcgcacg
     2761 gtgcgcgtgc gcgcctcgac cttgcgccgc atccgccgca accacagcgt ctcgagcggc
     2821 accagcacca gcaccagcaa ggacagcttc cacgacaggc tcagcatcag cgccaccgcg
     2881 ccgagcaggc cgatcacgct gctcaccgct gcgaacaggc tatccacagc gaaacgctgg
     2941 atctcggcga cgtcgccgtc gaggcgcgac agcaggtcgc ccatgcgctg acggccgtag
     3001 aaggcgggcg acagcgtctg caggtggcga tagaggtcgc tgcgcagcgc gaacaggatg
     3061 gagccggaca gacgggtgtg caattgccgg ttcaccccgg cgagcacggt gccgacgatg
     3121 ccgaccgcga tcatcgcgcc ggcgaccatc agcagggtgc ggtagtcgcg cgcgagcagg
     3181 ccctcgtcga tcagcagctt ggtcagccag ggctgcagca gcaccagcag ggtggccgcg
     3241 caggagagca gcagcaggcc ggcaatgcgg cggcgctgcg ggcgcacgaa gccgtacagc
     3301 cagcgcaggc cgcggcgcaa ctcgtcgggc cgctctgcat ggatcaggcc ggcgagcatg
     3361 acccggctcg cgacgacgac gcgtccatcg ggcccgctgc gcattggcgg tcgcgcgcga
     3421 cgaagaggcg gccaggcacg cgcatcgccc gcctcagctc gcgaccagca tgccgtgctc
     3481 gcgccgcgcc acgccggcgc gctcgagctc cttcaccagc caggcggcga gcgcatcggc
     3541 gtcgaggccg aggaagcgcg cgttggcggt gcggtacagc ggggtctcgt cgagatgccg
     3601 cggcagctcg acgagcgcca tgctgcgcac ctcgagcagc ttgaaggtga tgcaggcgcg
     3661 gatcgcgttg cgtgccatgc gcgcgccgtc gtcctcgaag gcgcggaggc gcgcgaaggc
     3721 gcgctcgagc gcgtcgtcga attcggcgaa gggggcccca tggccgggaa tcaccgcatc
     3781 caccgcgagc cggccgatcg cctccagggt gcggcgcgcc tcgccgagcg cgtcgccctg
     3841 gcccatcacc tcggcgaaca ggatgccgaa gccgtcgcgc cagagcgcat cgcccgagat
     3901 gagaatgcgg cgatcggggt tgtagtaggc gagcgcgtcc atgtcgtggc cgggggcggc
     3961 caccgcctgc cattcgagct cgccggcgac gaagcgcgcc ccggccgcga gcgtcgcgtc
     4021 atggcggaag cgctcgccgg cctgcgcgcc gacgctcagc agcagcgcct cctcgtccca
     4081 ctcctcgacc gcgcgcgcca tgccctcggg caccgtgatc gcgcacccga aggcgcgctg
     4141 cacgctggcg ttgccgccga tgtggtcgga atgcgagtgc gtgttgatga ggcggccgag
     4201 actgcggcca tcgagcgcgg cgcgcaccag cctcaccgtc tggtcggcat ggctcacgta
     4261 gccggagtcg atcagggtcg cctcgtcgcc gtcgaacagc agcacgttgt tggccgacag
     4321 ccagccgcgc tcgaagacct gcaggctggc aggcagatgc ggacgcgcgc tcaatcgctg
     4381 ccttccccga cgcgggccgc gacgttgggc ggcaagggca cgccggccgc ctgcgccggg
     4441 gacgccgccg catcgggctg ccccgcgacc tgcgctccgg gcgccggcgt gggcacgccg
     4501 ttgatctcgt cggcagtgcg accacagccc aggcacacgc ccgagtccca atcgatcatg
     4561 cacacgccga cacagagcgc gtcgccgctc atgtgcgcaa gtccacggcg ggggaagccg
     4621 cggccaggtg cgtgcggcgc tcggccacgg cggcgaggat ggcgcggcgg tcgtcgtcgg
     4681 tgctgcggct ccagcgcgtg atctcttcga tcgtgcgctg gcagccctcg caccagccgg
     4741 tgtcgggact catgcggcag aggttgatgc agggggaagg aacgctcatg gcagtcatct
     4801 ctgtagggcg gcgggcgggc catgcgtgtg gtccgcctcg tgatccggaa gccggattat
     4861 gaagcagtcg gcaagccatg gctcgcgagg accgcgttcg accgcctccc cgcccgcgtc
     4921 gcacgcaccc ttcgtcgctt cgtcccgcgc tcaggcgcag gccgtcttgg cgcgcttggc
     4981 cagcaccgca cgcgccacac gcgaggcagg ctcgcggcca agctgggcgc tgatccaggc
     5041 ggcggtgtcg acgagcgagt cgagatcgat gccggtgtcg atgccgagcc cgttcagcaa
     5101 ccagaccacg tcttcggtgg cgacgttccc cgaggcgccc gccgcatagg gacaaccgcc
     5161 caggccgccg accgaggcgt cgaacaccgc cacgcccatc tccagcgacg cgaagacgtt
     5221 ggccaccgcc atgccgtagg tgtcgtggta gtggccggcg agcttgtcca ccggcacgcg
     5281 cttcgccacc gcttcgagca tgcgccggat gccggccggg gtgccgacgc cgatggtgtc
     5341 gccgagcgag acctcgtagc agcccatctc cagcagcgtc gccgccacgt cggccaccgc
     5401 ctgcggcgcg accgccccct cgtaggggca acccaccacg caggagacgt agccgcgcac
     5461 cctcacgccg gccgcgtgcg ccgcgtcggt gaccgggcgg aagcgctcga gcgactcggc
     5521 aatcgagcag ttgatgttct tctggctgaa ggactcggag gcggcgccga acaccgcgac
     5581 ctcccgtgcc ccggccgcca gtgcggcctc gaagcccttg aggttggggg tcagcaccgg
     5641 gtaggcgaca ccgggcgccg cggcggcgag cacgcgcggc agcaactcgg cgttgtcgcc
     5701 catctgcggc acccacttcg gcgacacgaa ggaggtggtc tcgatcgcct tcagcccggc
     5761 gcgctcgagg cggcgcacga gctcgagctt ggtgtcggtg gagacgagct gtttctcgtt
     5821 ctgcaggccg tcgcgcggcc ccatctcgac gatgcggacg gcggcaggaa ggatggaatt
     5881 cgccatggag ggggtctcct caagaagcgt tcaattgggc tgcaggagca gcgatcaacc
     5941 gtcttggccg atgccgccac gccgacaaga acacgcgagc acgacagacc cgcgatcagg
     6001 cggtcttcgc ctcgtcgagc ccgagctctt ccttcatcct ctggcggatc aggaacttct
     6061 ggatcttgcc ggtcaccgtc atcgggaagc tgtcgacgaa gcggatgtag cgcgggatct
     6121 tgtagtgggc gatctggccc ttgcaatact cgcgcacctc gtcctcggtg aggcgctcgc
     6181 cctcgcgcgg gatgatccag gcgcacagct cctcgccgaa cttctggtcg gggatgccga
     6241 ccacctgcac gtcgagcacc ttggggtggg cgtagaggaa ctcctcgatc tcgcgcggat
     6301 agatgttctc gccgccgcgg atcaccatgt ccttgatgcg gccgacgatg ttgcagtagc
     6361 cctcgtcgtc gatcaccgcg aggtcgccgg tgtgcatcca gccgccggcg tcgatcgcct
     6421 ccttcgtcct ggcctcgtcg tcccagtaac cgagcatcac cgaatagcct cgggtgcaca
     6481 actcgccgga ctggccgcgc ggcacgatgc ggccctcgtt gtcgacgatc ttcacctcga
     6541 gatggggctg gatgcggccc acggtggaca cgcggcggtc gatggggtcg tcggtgccgc
     6601 tctggaagct caccggcgag gtctccgtca tgccgtaggc gatggtcacc tcggacatgt
     6661 tcatcttgcc gatcacccgc ttcatgacct cgatcggaca cgggctgccg gccatgatgc
     6721 cggtgcgcag gctgg
//
LOCUS       DAOQNN010000155         6705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6290_length_6705_cov_9.769925, whole genome shotgun sequence.
ACCESSION   DAOQNN010000155 DAOQNN010000000
VERSION     DAOQNN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6290_length_6705_cov_9.769925"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="PKV97_09490"
     CDS             complement(<1..1560)
                     /locus_tag="PKV97_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB06147.1"
                     /translation="MRLSKLKLAGFKTFVDPTTVLTPGNLVGVVGPNGCGKSNIIDAV
                     RWVLGETRASALRGESMQDVIFNGSTTRKPVSRASVELVFDNAEGRAARQWSRYAEIS
                     VKRVLDRSGESTYYINNVHVRRKDVIDLFLGTGLGPRAYAIIEQGMISRIIEARPEEI
                     RGFLEEAAGVTKYRERRKETEGRLRDARDNLARLDDIRMELGERIVHLEAQAAVAARY
                     RELDAAHVEKQQLLWLVKRNEARAEQARVAASLNEASSRIEADSARLQELETSVESRR
                     DAHFEASEAVHVAQNDLFAASAEVARLETELQHLGEARRRLEARLAQLELDRGHWSSR
                     RETLAADRARWQELAENAALRAEHAEARHLEIADRLPELDSSRQGADATMAAARRELA
                     QTEQQLRVEETKRASALRALEALQQRRGRLEGERGGIVGPDERVLAEREARLEALQDE
                     LEGHQQELAAAQPRLPDAQAALKAALEHERAVQRRLTELRARRDALMQLQARVQSSMY
                     VLPRTCSPSPVR"
     gene            1827..3020
                     /gene="dapC"
                     /locus_tag="PKV97_09495"
     CDS             1827..3020
                     /gene="dapC"
                     /locus_tag="PKV97_09495"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HNB06148.1"
                     /translation="MNPNLARLQSYPFEKLRALFAGVEPPAHLKPIRLSIGEPQHPTP
                     GFIRQTLADNLGGLSSYPATLGSDALRGAIAHWLERRYGLPKIDPASQVIPVAGTREA
                     LFAFAQCVVDGTKPGAKVLCPNPFYQIYEGAALLANAEPIFINNLPESDFGSDFGSID
                     EATWRDIQLVYVCSPGNPTGRLLTLDEWKTLFELSDRYGFVIAADECYSEIYFDDADK
                     PIGGLEAAWRLGRTDFRNVVMFSSLSKRSNVPGMRSGFVAGDAKILGDFLLYRTYHGC
                     ALNPAVQAASVVAWNDEEHVEENRRLYRDKFARITPMLQPWLPVRLPDAGFYYWVRTP
                     LPDTEFARRLQAEYNVTVLPGSYLARESNGVNPGAGFVRIALVADTHECVEAAGRIID
                     FCKTL"
     gene            3035..3856
                     /gene="dapD"
                     /locus_tag="PKV97_09500"
     CDS             3035..3856
                     /gene="dapD"
                     /locus_tag="PKV97_09500"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNB06149.1"
                     /translation="MHALQSIIDTAFENRASLSPTSAPAEIRNAVEEVIAGLDAGTLR
                     VAEKKDGQWVVNQWIKKAVLISFRLRDNEVVQAGGLNFFDKVATKFGDYTPEQFREGG
                     FRVVPPAVARKGSFIGRNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPVQAGPVIIEDNVFVGARSEVVEGVIIEENAVLSMGVYIGMSTKIYDRE
                     TGEITYGRVPAGAVVVPGSLPSACGKYSLYCAVIVKKVDAQTRAKTGINELLRGA"
     gene            3930..5102
                     /locus_tag="PKV97_09505"
     CDS             3930..5102
                     /locus_tag="PKV97_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNB06150.1"
                     /translation="MIFDNLFQLMAEKKASDIFITAGTPIHIKIQGHTMPINQQVMDP
                     LMIQRMLHEAITPAQQAVLERERELNLSFGRRDLGNFRVNVFWQRNSMAVVVRYIQSD
                     IPSIGALGLPPVLDDIVLEKRGLVLVVGATGSGKSTTLAAMIDHRNRNRSGHILTIED
                     PIEYLFKHRKSVVNQREVGIDTLSWGEALRNAMRQAPDCILIGEIRDRETMQAAISYS
                     QTGHLCLATLHANNAYHALNRIVNFFPLENRQLLYLDLAVALRCVISQRLVRKPDGKR
                     IPAVEILMNTRHIAELVERGELNAIKEAMEQSLAQGSQTFEQALYRLHHDNIITLEEA
                     LANADSPSNLHWLINNAQMQSDPMLTVNGNSAISGFEATNTEGASFQEFTLDLNNN"
     gene            5127..6284
                     /gene="dapE"
                     /locus_tag="PKV97_09510"
     CDS             5127..6284
                     /gene="dapE"
                     /locus_tag="PKV97_09510"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HNB06151.1"
                     /translation="MQTSPSSSSTLALACELIARPSVTPEDAGCLELVASRLAPLGFR
                     LERIDSGGVCNLWARRGSAAPVLCFAGHTDVVPPGPDSAWSSPPFAPTIRDGVLYGRG
                     AADMKSSLAAFVTAIERFVAAHPDHAGSIALLLTSDEEGIATHGTVKVVEALAARGER
                     LDYCVVGEPTSVQTLGDMIKNGRRGSLSGTLRVKGQQGHVAYPQLARNPIHLFAPALA
                     ELAASDWDQGNEFFPPTTWQVSNIHAGTGANNVIPGVCEVMFNFRFGSVSSADELKSR
                     TCEVLDRHGLDYEIDWHLSGKPFITGRGRLVGALSAAIRETLGVETELSTTGGTSDGR
                     FIAEICAEVVEFGPVNASIHKIDEHIAADAIEPLSVVYERTLTALLVPGRL"
     gene            6344..>6705
                     /locus_tag="PKV97_09515"
     CDS             6344..>6705
                     /locus_tag="PKV97_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNB06152.1"
                     /translation="MANQQHADDDNLALEQHDDHEHEHGPLAELVTVRDWLRYAVTRF
                     NRAGVFCGHGVQDSFDEAVWLILGSLALPLDRLEPFMDACIPSEERIGLLEAIEQRVD
                     ERVPTAYILGEAWLGDFR"
BASE COUNT         1066 a         2444 c         2146 g         1049 t
ORIGIN      
        1 cctgaccggc gacgggctgc acgtccgcgg caacacgtac atcgacgact gcacgcgcgc
       61 ctgcagctgc atcagggcgt cgcgacgcgc gcgcagctcg gtgagccggc gctgcaccgc
      121 acgctcgtgc tccagtgccg ccttcagcgc ggcctgcgcg tcgggcaggc gcggctgcgc
      181 cgcggcgagt tcctgctggt gcccctccag ctcgtcctgc agcgcttcga gccgcgcctc
      241 gcgctcggcg agcacacgct cgtccgggcc gacgatcccg ccgcgctcgc cctccaggcg
      301 gccgcgacgc tgttgcaggg cttcgagcgc acgcaaggca ctcgcgcgct tggtttcctc
      361 gacgcgcaat tgctgctcgg tctgggcgag ctcgcggcgc gccgccgcca tggtcgcgtc
      421 ggcgccctgg cgcgaagagt ccagctcagg caggcggtcg gcgatctcca ggtggcgggc
      481 ctcggcgtgc tcggcgcgca gcgctgcgtt ctcggcgagt tcctgccagc gcgcgcggtc
      541 ggcggcgagc gtctcgcggc gcgaggacca gtgcccgcgg tcgagttcga gctgcgccag
      601 gcgcgcctcc agccgcctgc gcgcctcgcc gagatgctgc agctcggtct ccaggcgtgc
      661 gacctcggcg ctcgccgcga acaggtcgtt ctgcgccacg tgcaccgctt cggaggcctc
      721 gaagtgcgcg tcgcgccgcg actccacgct ggtctcgagc tcctgcaggc gggcgctgtc
      781 ggcctcgatg cggctgctcg cctcgttcag ggaggccgcc acgcgcgcct gctcggcgcg
      841 cgcctcgttg cgcttgacca gccacaagag ctgctgcttc tcgacgtgcg ccgcgtcgag
      901 ctcgcggtag cgcgccgcca ccgcggcctg cgcctcgagg tgcacgatgc gctcgccgag
      961 ctccatgcgg atgtcgtcga ggcgggcgag gttgtcgcgc gcgtcgcgca gccgaccctc
     1021 ggtctccttg cgccgctcgc ggtacttggt gacgcccgcg gcctcctcca ggaagccacg
     1081 gatctcttcc gggcgcgcct cgatgatgcg cgagatcatg ccctgctcga tgatcgcgta
     1141 agcgcgcgga cccagaccgg tgcccaggaa gaggtcgatc acgtccttgc ggcggacatg
     1201 gacgttgttg atgtaatagg tcgactcgcc cgagcggtcg agcacacgct tcaccgagat
     1261 ctcggcatag cgcgaccact gcctggcggc cctgccctcg gcgttgtcga agaccagctc
     1321 gacactcgcg cgcgacaccg gcttgcgcgt ggtcgagccg ttgaagatca cgtcctgcat
     1381 cgactcgccg cgcaacgcgg acgcgcgcgt ctcgcccagc acccagcgca cggcgtcgat
     1441 gatgttcgac ttgccgcagc cattgggccc gaccactccg acgaggttgc cgggggtgag
     1501 cacggtggtg ggatcgacga aggtcttgaa accggcgagt ttgagcttgg agagacgcac
     1561 agcgggcagt gggaagacag cgggacgtgc aggccggcga tccaccccgc cgcactccgc
     1621 ttttgcggcc ggcatcatac caccctgccg aagtcatccc gtgcgtccgc ctcgcatcga
     1681 aacgttacgc agcggattca ggcgtcgccg ccgcgcagcg cggtcctgta cccggattcg
     1741 cgccgtcgcc ttccgacggg cgccgccgcc atacgcaggt atactcggcg cccgcgtcca
     1801 ttgccggcca aggagcccgc gtacccgtga atcctaacct cgcccgcctg cagagctatc
     1861 ccttcgaaaa gctgcgcgcc ctcttcgccg gtgtcgagcc gcctgcgcac ctcaagccga
     1921 tccggctgtc gatcggcgag ccgcagcacc ccacccccgg tttcatccgc cagaccctgg
     1981 ccgacaacct cggcggcctg tcgagctatc ccgccacgct cggcagcgac gcgctgcgcg
     2041 gcgcgatcgc gcactggctc gagcgccgct acgggctgcc gaagatcgac ccggccagcc
     2101 aggtgatccc ggtcgcgggc acgcgcgagg cgctcttcgc cttcgcgcag tgcgtggtcg
     2161 acggaacgaa gccgggcgcc aaggtgctgt gcccgaaccc cttctaccag atctacgagg
     2221 gcgcggccct gctcgccaac gccgagccga tcttcatcaa caacctgccc gagagcgact
     2281 tcggctcgga cttcggcagc atcgacgagg ccacctggcg cgacatccag ctcgtctacg
     2341 tgtgctcgcc tggcaatccc accggccggc tgttgacgtt agacgagtgg aagacgctgt
     2401 tcgagctctc ggaccgctac ggcttcgtca tcgccgccga cgagtgctat tcggagatct
     2461 atttcgacga cgccgacaag ccgatcgggg gcctggaggc ggcctggcgc ctcgggcgca
     2521 ccgacttccg caacgtggtg atgttctcca gcctgtccaa gcgctcgaac gtgcccggca
     2581 tgcgctcggg cttcgtcgcg ggcgacgcga agatcctcgg ggatttcctg ctctatcgca
     2641 cctaccacgg ctgcgccctc aaccccgcgg tgcaggcggc ctcggtggtg gcctggaacg
     2701 acgaagagca cgtcgaggaa aaccgccgcc tctaccgcga caagttcgcc cgcatcacgc
     2761 ccatgctgca gccctggctg ccagtgcgcc tgcccgatgc cggcttctac tactgggtgc
     2821 gtacgccgct gcccgacacc gagttcgccc gccgtctgca ggctgaatat aatgtgacgg
     2881 tcctgccggg cagctacctg gcccgcgaat ccaatggcgt caacccgggc gccggcttcg
     2941 tgcgcatcgc gctggtggcc gacacccacg aatgtgtcga ggccgccggg cgcatcatcg
     3001 atttctgcaa aaccctctga gagagaatcc cccaatgcac gctctgcaat cgatcatcga
     3061 caccgccttc gagaaccgcg ccagcttgtc gcccacttcc gccccggcag agatccgcaa
     3121 cgcggtcgaa gaggtcatcg ccggcctgga tgccggcacg ctgcgcgtgg ccgagaagaa
     3181 ggacggccag tgggtggtca accagtggat caagaaggcc gtgctgatct ccttccgcct
     3241 gcgcgacaac gaggtggtgc aggccggcgg cctcaacttc ttcgacaagg tggcgaccaa
     3301 gttcggcgac tacacccccg agcagttccg cgagggtggc ttccgcgtgg tgccgccggc
     3361 ggtcgcccgc aaaggcagct tcatcggccg gaacgtcgtg ctgatgccgt cctacgtgaa
     3421 catcggcgcc tatgtggacg aaggcacgat ggtcgacacc tgggccaccg tcggttcgtg
     3481 cgcgcagatc ggcaagaacg tgcacctgtc gggcggcgtg ggcatcggcg gcgtgctcga
     3541 gccggtgcag gccggaccgg tgatcatcga ggacaacgtc ttcgtgggcg cgcgctccga
     3601 ggtcgtggag ggcgtgatca tcgaggagaa cgcggtgctg tcgatgggcg tgtacatcgg
     3661 catgagcacc aagatctacg accgcgagac cggcgagatc acctacggcc gcgtgccggc
     3721 gggcgccgtc gtggtgcccg gcagcctgcc ttcggcctgc ggcaagtaca gcctgtactg
     3781 cgcggtgatc gtcaagaagg tggacgcgca gacccgcgcc aagaccggca tcaacgagct
     3841 gctgcgcggc gcctgagcgg cgcactccgg ggaccggcct gcgcgcggtt cccgggcaaa
     3901 tccttgggtg acgacaggag tccgaaggcg tgatcttcga caatctcttc cagttgatgg
     3961 ccgaaaagaa agcttcggac atcttcatca ccgccggcac cccgatccac atcaagatcc
     4021 agggccacac catgccgatc aaccagcagg tcatggaccc gctgatgatc cagcgcatgc
     4081 tgcatgaggc gatcaccccg gcccagcagg cggtgctcga acgcgagcgc gagctcaacc
     4141 tgtccttcgg tcggcgcgat ctcggcaact tccgcgtcaa cgtgttctgg cagcgcaact
     4201 cgatggccgt ggtggtgcgc tacatccaga gcgacattcc gagcatcggc gcgctcggcc
     4261 tgccgccggt gctggacgac atcgtgctgg agaagcgcgg cctggtgctc gtggtgggcg
     4321 cgaccggctc gggcaagtcc accacgctcg cggccatgat cgaccaccgc aaccgcaacc
     4381 gcagcggcca tatcctcacc atcgaggatc cgatcgaata cctcttcaag caccgcaaat
     4441 cggtggtcaa ccagcgcgag gtgggcatcg acaccctgag ctggggtgag gcgctgcgca
     4501 acgcgatgcg ccaggcgccc gactgcatcc tgatcggcga gatccgcgac cgcgagacca
     4561 tgcaagccgc catctcgtac tcgcagaccg gccacctgtg cctggcgacg ttgcatgcca
     4621 acaacgctta tcacgcgctc aatcgcatcg tcaacttctt cccgctggag aaccgccagc
     4681 tgctctacct cgacctcgcg gtggcgctgc gctgcgtgat ctcccagcgc ctggtgcgca
     4741 agcccgacgg caagcgcatc ccggccgtcg agatcctcat gaacacgcgg cacatcgccg
     4801 agctggtcga gcgcggcgag ctcaatgcga tcaaggaggc catggagcag tcgctcgccc
     4861 agggctcgca gaccttcgag caggcgctct accgtctcca ccacgacaac atcatcaccc
     4921 tcgaggaggc gctcgccaat gccgactcgc cgagcaacct gcactggctg atcaacaacg
     4981 cgcagatgca gagcgatccg atgctgaccg tcaacggcaa cagtgccatc tccggcttcg
     5041 aggcgaccaa cacggaaggt gcctccttcc aggagttcac cctggacctc aacaacaact
     5101 aggcgcccgc gcggccgccc tcccccatgc agaccagccc ctccagctcc tccaccctcg
     5161 cgctcgcctg cgagctcatc gcccgcccct ccgtcacgcc ggaggacgcc ggctgcctcg
     5221 aactcgtcgc ctcccgcctt gccccgctcg gcttccgcct cgaacgcatc gacagcggcg
     5281 gcgtctgcaa cctgtgggcg cggcgcggca gcgcggcgcc cgtgctgtgc ttcgccggcc
     5341 acaccgacgt cgtgccgccc gggccggaca gcgcgtggtc ctcgccgccc ttcgcaccta
     5401 cgatccgcga cggcgtgctg tacggtcgcg gtgcggccga catgaagtcc tcgcttgccg
     5461 ccttcgtcac cgccatcgaa cgcttcgtcg ccgcccatcc cgaccacgct ggcagcatcg
     5521 cgctgctgct gacgtcggac gaggaaggca tcgccaccca tggcacggtc aaggtggtcg
     5581 aggccttggc cgcgcgcggc gagcgcctcg actactgcgt ggtgggcgag ccgacctcgg
     5641 tgcagacgct cggcgacatg atcaagaacg gccgccgcgg ctcgctgtcg ggtacgctgc
     5701 gggtcaaggg ccagcagggc cacgtcgcct acccgcagct cgcccgcaac ccgatccacc
     5761 ttttcgcgcc ggcgctcgcc gagctcgcgg cgagcgactg ggaccagggc aacgagttct
     5821 tcccgcccac gacctggcag gtgtcgaaca tccacgccgg caccggcgcc aacaacgtga
     5881 tccccggtgt gtgcgaggtg atgttcaatt tccgcttcgg ctcggtgtcg agcgcggacg
     5941 agctgaaaag ccgcacttgc gaggtgctcg accgccacgg gctggactac gagatcgact
     6001 ggcacctgtc cggcaagccc ttcatcaccg ggcgcggcag gctggtcggc gcgctgtcgg
     6061 ccgcgatccg ggagacgctg ggagtcgaga cagaactgtc gacgaccggc ggcacctccg
     6121 acggccgctt catcgccgag atctgcgccg aggtagtcga gttcggcccg gtcaatgctt
     6181 cgatccacaa gatcgacgag cacatcgcag ccgatgcgat cgaacccttg tccgtggttt
     6241 atgagcgcac cttgaccgcc ctgctcgtcc ccggccgcct gtaggagcga cgcaagtcgc
     6301 gatgacggcc ccctccgccc ccaatctacc gaacagactt gccatggcca accaacaaca
     6361 cgccgacgac gacaacctcg cccttgaaca gcacgacgac cacgagcatg aacacggccc
     6421 gctcgccgag ctcgtgaccg tgcgcgactg gctacgctac gccgtcaccc gcttcaatcg
     6481 cgccggcgtc ttctgcggcc atggcgtgca ggacagcttc gacgaggcgg tgtggctgat
     6541 cctcggcagc ctcgccctgc cgctcgaccg cctggagccg ttcatggacg cctgcatccc
     6601 gtccgaggag cgcatcggcc tgctcgaagc catcgagcag cgcgtcgacg agcgcgtccc
     6661 caccgcctac atcctcggcg aggcctggct gggcgacttc cgctt
//
LOCUS       DAOQNN010000156         6695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6309_length_6695_cov_8.501355, whole genome shotgun sequence.
ACCESSION   DAOQNN010000156 DAOQNN010000000
VERSION     DAOQNN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6309_length_6695_cov_8.501355"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKV97_09520"
     CDS             <1..358
                     /locus_tag="PKV97_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB06153.1"
                     /translation="TPPTQPAAAMPQRPPAPVATPEPRRPSADEEARLRALIEGKASP
                     ARGDSYVLQIGAFSDAVKAARLVDDLRARGFPAYTERAGNVMRVRVGPVAGRAEADAT
                     AARLKAAGHPAVLQPR"
     gene            370..861
                     /locus_tag="PKV97_09525"
     CDS             370..861
                     /locus_tag="PKV97_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNB06154.1"
                     /translation="MTVFDYVFLGLLAFSALIGMWRGLVSEVMAVLAWAAALFAAWRY
                     NEEAAGVFTGMIVEPMWRQVAGGALVVVGVLILAALLRYLLRELLRAAGLGATDRFFG
                     ALFGIARGLAVAFVVVLIGGLAGVSKEPWWRDALFAAPLESAVIAAKPWLPAEVADKI
                     RFR"
     gene            872..2407
                     /gene="purF"
                     /locus_tag="PKV97_09530"
     CDS             872..2407
                     /gene="purF"
                     /locus_tag="PKV97_09530"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNB06155.1"
                     /translation="MCGILGVVATSPVNQLLYDGLQVLQHRGQDAAGIATSEGGRFHM
                     HKGSGLVRDVFRTRNMRNLQGNWGIGHVRYPTAGSAYNAAEAQPFYVNSPFGLLLAHN
                     GNLTNSEALKREMFLSDLRHINTSSDSEVLLNVFAHELQAACKGLKIDEEAIFTAVAG
                     VHRRCRGAYAVVVMIAGYGLLAFRDPYGIRPLVIGRNDCEAGTEWLVASESVAVDVLG
                     FRLLRDVAPGEAVLVDTAGRFHSRQCAARTVHAPCMFEFVYLARPDSIIDGVSVYESR
                     MKMGEFLADKMRRTMPNVHIDVVIPIPDSSRPSAMQMADRLDLPFREGFVKNRYIGRT
                     FIMPGQATRRKSVRQKLNTIHQEFKGKSVLLVDDSIVRGTTSKEIVNMAREAGATKVY
                     LASAAPPVRYANVYGIDMPTRGELIASDRSEEEICREIGANGLIYQDLDDLKASVRAL
                     NPSLQFFETSCFDGHYITGDITVEYLNGVENQRSEAKPGKNDQDDGDDGQLDLNLASD
                     AEH"
     gene            2441..3625
                     /locus_tag="PKV97_09535"
     CDS             2441..3625
                     /locus_tag="PKV97_09535"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HNB06156.1"
                     /translation="MNRPLPPELHPDTLAVRAGTERTQFREHGEAMYLTSSFVFDSAA
                     QAAACFSGEEEGYVYARFSNPTVTAMQNRLAALEGGEACIATASGMSAILSLAMATMQ
                     AGDHVVASNGLFGATQQLFGGILSKFGITTSFVPATELDAWRAAITPRTRLFFTETPS
                     NPLTEVIDIAGVAAIARAAGVIFAVDNCFCTPALQRPLELGADVVVHSATKYLDGQGR
                     VLGGAVVGSKAITDEVFKFLRTAGPTLSPFNAWVILKGLETLRIRMEAQSASALELAR
                     WLEAQPGVARVYYPGLESHPQHALAMRQQKSGGAIVSFDVKGGREAAWKVVDATRLIS
                     ITANLGDTKSTITHPATTTHGRISAEARATAGIGDGLLRIAVGLEDVDDLKADLARGL
                     AG"
     gene            complement(3943..4512)
                     /locus_tag="PKV97_09540"
     CDS             complement(3943..4512)
                     /locus_tag="PKV97_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNB06157.1"
                     /translation="MGHAQEAATLNRQAYLDWEAAQPDKHEYLAGEVFAMVGVRRGHA
                     LAALAVASRLRAHLKGSRCDTFVSDMKLFVADADAYFYPDVMVTCDERDRRAELAIEH
                     PRLVVEVLSDSTAAYDRGIKFAACRKLADVQEYLLVDVDQRRLELYRRDAGHWILLES
                     EGDGPALRLESVDMTLSPAEAFEDLDGEA"
     gene            4665..5384
                     /locus_tag="PKV97_09545"
     CDS             4665..5384
                     /locus_tag="PKV97_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNB06158.1"
                     /translation="MKTIGLLGGMSWESTVPYYRQINEAVKARLGGLHSARIVLYSVD
                     FAEIERLQHAGDWDAAGAVLAAAAQALERAGADFIVLCTNTMHKVAPAIEAATNIPLL
                     HIADPTAAAIRHAGLSRVGLLGTRFTMEQAFYRGRLEERHGIAVLVPAADDRDRVHRV
                     IYEELCLGRVEDASRAAYRAAMRRLVERGAQGIILGCTEISMLVSAQDANVPLFDTTA
                     IHAAAAVELALGEGQEGGAVL"
     gene            5593..6120
                     /locus_tag="PKV97_09550"
     CDS             5593..6120
                     /locus_tag="PKV97_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06159.1"
                     /translation="MKELIKRLRKILDTVQMADDLRKRLERLLEALEGGEPTDIVIAR
                     LLADLLELGADRLPGPVGDFVKAYAEAFRNAIDAILAVLWKRYREARRAGLDHQEAND
                     LVTFDAKVCEWLRFRWLLEQFDESREAAGDGERGKRYTWRRLQVSAQEPREILVHVRA
                     VSACIGVLDRMVRIG"
     gene            complement(6146..6403)
                     /locus_tag="PKV97_09555"
     CDS             complement(6146..6403)
                     /locus_tag="PKV97_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNB06160.1"
                     /translation="MKVLIPTPLLSYTGVREVDAEGATLDAVLTDLDRRYPGIRFRVI
                     DEQGRLRRHVRFFADGGQIFDPAHPMRADDTLVLVQALSGG"
     gene            complement(6400..>6695)
                     /locus_tag="PKV97_09560"
     CDS             complement(6400..>6695)
                     /locus_tag="PKV97_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNB06161.1"
                     /translation="DVWPRTSPGGRPAVYGTRDGGASWQRLDRGLPPQQAWWTVKRQS
                     MCADGLDPLGLYFGTTSGELWTSADEGEHWQCIARHLPEILAVETGHPSAAAT"
BASE COUNT         1040 a         2400 c         2229 g         1026 t
ORIGIN      
        1 cacgccaccc acgcagcccg ccgcggcgat gccgcagcgc ccgcctgcgc cggtggcgac
       61 ccccgagccg cgccgcccct ccgccgacga agaggcccgc ctgcgcgcgc tcatcgaggg
      121 caaggcctcg cccgcgcgtg gagacagcta cgtgctgcag atcggtgcct tcagcgacgc
      181 cgtcaaggcc gccaggctgg tcgatgatct gcgcgcgcgc ggcttcccgg cctacaccga
      241 gcgcgccggc aacgtgatgc gggtgcgtgt cggtccggtg gccggtcgcg ccgaggcgga
      301 cgccaccgcg gcccgcctca aggccgccgg acacccggcg gtcctgcagc cgcgctgact
      361 tcccggatca tgaccgtctt cgactacgtc tttctcggcc tgctcgcctt ctccgcgctc
      421 atcggcatgt ggcgcggcct ggtcagcgag gtcatggcgg tgctcgcgtg ggcggcagcg
      481 ctcttcgccg cgtggcgcta taacgaagaa gcggccgggg tcttcaccgg catgatcgtc
      541 gaaccgatgt ggcgccaggt cgcgggtggt gcgctggtcg tggtcggggt gctgatcctc
      601 gccgcgttgc tgcgttacct gttgcgcgag ttgctgcgcg ccgccgggct gggcgccacc
      661 gaccgcttct tcggcgcgct cttcggcatc gcgcgcgggc tggcggtcgc cttcgtggtg
      721 gtgctgatcg gcgggctcgc cggcgtttcc aaggagccgt ggtggcgcga cgcgctgttc
      781 gcggcgccgc tggagtcggc ggtgatcgcc gccaaaccct ggctgcctgc agaggtagcc
      841 gacaagattc gattcagata ggcctcaatc catgtgcgga attctcggtg tcgtcgccac
      901 ctccccggtc aaccaactgc tctatgacgg cctccaggtg ttgcagcacc gcggccagga
      961 cgcggcgggc atcgccacct cggagggcgg gcgtttccac atgcacaagg gctccggcct
     1021 cgtgcgcgac gtgttccgca cgcgcaacat gcgcaatctc cagggcaact ggggtatcgg
     1081 ccatgtgcgc tacccgaccg cgggctcggc ctacaacgcc gccgaggcgc agcccttcta
     1141 cgtcaactcg cccttcggcc tgttgctggc gcacaacggc aacctgacca actcggaagc
     1201 gctcaagcgc gagatgttcc tgtccgacct gcgccacatc aacaccagca gcgactccga
     1261 ggtgctgctc aacgtcttcg cacacgagct gcaggccgcc tgcaaggggc tcaagatcga
     1321 cgaggaggcc atcttcaccg cggtcgccgg cgtgcatcgg cgctgccgcg gcgcctacgc
     1381 ggtggtggtg atgatcgccg gctacggtct gctcgccttc cgcgaccctt acggcatccg
     1441 ccccctggtg atcggccgca acgactgcga ggccggcacc gagtggctgg tggcctcgga
     1501 gtcggtggcg gtggacgtgc tcggcttccg cctgctgcgc gacgtcgcgc cgggcgaggc
     1561 ggtgctggtc gataccgccg gccgcttcca cagccgccag tgcgccgcgc gcacggtgca
     1621 tgcgccgtgc atgttcgagt tcgtctacct ggcacggccg gattcgatca tcgacggcgt
     1681 gtcggtgtac gagtcgcgca tgaagatggg cgagttcctc gccgacaaga tgcgccgcac
     1741 catgcccaac gtgcacatcg acgtggtgat cccgatcccc gactccagcc gcccctcggc
     1801 gatgcagatg gccgaccggc tcgacctgcc cttccgcgag ggcttcgtca agaaccgcta
     1861 catcggccgc accttcatca tgcccggcca ggccacgcgc cggaagtcgg tacgccagaa
     1921 gctcaacacc atccaccagg agttcaaggg caagagcgtg ctgctggtcg acgactccat
     1981 cgtgcgcggc acgaccagca aggagatcgt caacatggcg cgcgaggcgg gggccaccaa
     2041 ggtctatctc gcctcggccg cgccgccggt gcgctacgcc aacgtctacg gcatcgacat
     2101 gcccacgcgc ggcgagctga tcgcctccga ccgcagtgaa gaggagatct gccgcgagat
     2161 cggcgccaac ggcctcatct accaggacct cgacgacctc aaggcctcgg tgcgtgcgct
     2221 caacccctcg ctgcagttct tcgagacctc gtgtttcgac ggccactaca tcaccggcga
     2281 catcaccgtc gagtacctca acggcgtcga gaaccagcgc agcgaagcca agccgggcaa
     2341 gaacgaccag gatgacgggg acgatggcca gctcgacctg aacctcgcct ccgacgccga
     2401 gcactgagcc gcccccaaga cgaaaccaag cacgaccacg atgaaccgcc cccttccgcc
     2461 cgagcttcac cccgacaccc tggccgtgcg cgccggcacc gagcgcacgc agttccgcga
     2521 gcacggcgag gcgatgtacc tcacctcgag cttcgtcttc gacagcgccg cgcaggccgc
     2581 cgcctgcttc tcgggcgagg aggagggcta cgtctacgcg cgcttctcca accccaccgt
     2641 gaccgcgatg cagaaccggc tcgccgcgct cgaaggcggc gaggcctgca tcgccaccgc
     2701 gtccggcatg tccgcgatcc tgtcgctggc gatggcgacc atgcaggcgg gcgaccacgt
     2761 ggtggcctcc aacgggctgt tcggcgccac ccagcagctc ttcggcggca tcctgtcgaa
     2821 gttcggcatc accaccagct tcgtgccggc caccgagctc gacgcctggc gcgccgcgat
     2881 cacgccgcgc accaggctct tcttcaccga gacgccctcc aacccgctca ccgaggtgat
     2941 cgacattgcc ggcgtggcgg cgatcgcgcg cgcggcgggg gtgatcttcg cggtcgacaa
     3001 ctgcttctgc acgccggcgc tgcagcgccc gctggagctc ggcgccgacg tcgtcgtgca
     3061 ttcggccacc aagtatctcg acggccaggg cagggtgctc ggcggcgcgg tggtcggcag
     3121 caaggcgatc accgacgaag tgttcaagtt cctgcgcacc gccgggccga ccctgtcgcc
     3181 gttcaacgcc tgggtgatcc tcaagggcct ggagacgctg cgcatccgca tggaggcgca
     3241 gtcggcgagt gcgctcgagc tggcgcgctg gctcgaggcc cagccgggcg tggcgcgggt
     3301 gtactacccc gggctggaat cccaccccca gcacgcgctg gccatgcgcc agcaaaagag
     3361 cggcggcgcc atcgtcagct tcgacgtgaa gggcggccgc gaggcggcgt ggaaggtggt
     3421 cgacgccacc cgcctgatct cgatcaccgc caatcttggc gacaccaaga gcaccatcac
     3481 ccaccccgcg accaccaccc acggccgcat cagcgccgag gcgcgcgcca ccgccggcat
     3541 cggcgacggc ctgctgcgca tcgccgtcgg tctggaagac gtcgacgacc tcaaggccga
     3601 cctcgcccgc ggcctggcgg gctgacgcgc gtccgtcgca gatcacaccg gcctctgccc
     3661 gaagcgttcg agcctccttc aggcgcgacg caagtcgtat gggttcctgt aggagcgacg
     3721 cgagtcgcga gtcgcgatgc gcggcgccga tgcggatcgc cgtcattcca gcgcccgatc
     3781 gcgacttgcg tcgctcccac ggaaaacccg ccacagcccg ggcctgtatg taggagcgac
     3841 gcaagtcgcg atccagcgcg catccacgca agacatcgcg ccggaaaacc tcaccggaac
     3901 catccctggc ctgccgcact cgcgaccaag cctgcgtcgc cttcaggcct cgccgtccag
     3961 atcctcgaac gcctccgccg gcgacagcgt catgtccacg ctctccaggc gcagggcggg
     4021 cccgtctcct tcgctctcga gcaggatcca gtgcccggcg tcgcggcggt acagctcgag
     4081 gcggcgctga tcgacgtcga ccagcaggta ttcctgcaca tccgccagtt ttcgacaagc
     4141 ggcgaacttg atcccgcggt cgtaggcggc ggtggagtcc gacaacacct cgaccaccag
     4201 ccgcggatgc tcgatcgcga gctcggcacg gcggtcgcgc tcatcgcagg tgaccatcac
     4261 gtccgggtag aaataggcgt cggcatcggc gacgaagagc ttcatgtcgg agacgaaggt
     4321 gtcgcagcgc gagcccttga ggtgcgcgcg cagtcgactc gcgacagcca gcgcggccag
     4381 cgcatgcccg cgcctcaccc ccaccatcgc gaacacttcg ccggccagat actcgtgctt
     4441 gtccggctgc gcggcctccc agtcgaggta ggcctgccga ttcagtgtcg cggcttcctg
     4501 tgcgtgtccc attgcgaagc tccagtcgat gtcccgccat tgtgggcgag gagggcggcg
     4561 gcttcaacgc tacacgcgtg caaggccgcg gatccccggc tgccgatcgc gcccgatccc
     4621 catggcatca tgtctgcccc cacatcttcc cggacccgca acccatgaag accatcggcc
     4681 tgctcggcgg catgagctgg gaatccaccg tgccctacta ccgccagatc aacgaagcgg
     4741 tgaaggcccg cctcggcggc ctgcactcgg ccaggatcgt gctgtacagc gtcgacttcg
     4801 ccgagatcga gcgcctgcag cacgccggcg actgggacgc cgccggcgcc gtgctcgccg
     4861 cggccgcgca ggcgctggag cgcgccggtg ccgacttcat cgtgctgtgc accaacacca
     4921 tgcacaaggt ggcccccgcc atcgaggccg cgacgaacat cccgctgctc cacatcgccg
     4981 acccgaccgc cgcggccatc cgccacgccg gcctgtcccg cgtcggcctg ctcggcacgc
     5041 gcttcaccat ggagcaggcc ttctaccgcg gccggctgga ggagcgccac ggcatcgcgg
     5101 tgctggtccc cgcagcggac gaccgcgacc gcgtgcatcg cgtgatctac gaggagctct
     5161 gtctcggccg cgtcgaagac gcctcgcgcg ccgcctaccg cgcggcgatg cgccgcctgg
     5221 tcgaacgcgg cgcgcaaggc atcatcctcg gctgcaccga gatctcgatg ctggtgtccg
     5281 cgcaggacgc gaacgtgccg ctgttcgaca ccaccgcgat ccatgcggcg gcggcggtgg
     5341 agcttgcgct cggggagggg caggaggggg gcgccgtgtt gtagcgccgg cgaaccctgg
     5401 gtggtgggac gttcgggcaa tgcgtacggc cgcgtccgcg gggataggcc tgaaggcttt
     5461 tgcgcgctgc ccttacatct cgcgtaaagg tcgatggcgc tcgtctatgc tgaaacaagg
     5521 ggatgacggg tgtccgcgtc ggtcggcatt ggccgcggag cgtggccccg gctgcatgtg
     5581 gaagggtgag ccatgaaaga actcatcaag cgcttgagga agatcctcga cacggtccaa
     5641 atggccgacg atcttcgtaa acgcctcgaa aggctgctgg aggccctcga gggcggggaa
     5701 ccgaccgata tcgtcattgc ccgcttgctg gctgatttgc tcgagctggg tgcggaccgg
     5761 cttcccggcc cggtgggcga cttcgtcaag gcttatgcgg aggcattccg aaacgccatc
     5821 gatgcgattc tcgccgtgct gtggaagcgt taccgcgagg cacgccgggc cggactcgac
     5881 caccaggagg ccaatgacct cgtcaccttc gatgcgaagg tctgcgaatg gctccgcttc
     5941 cgctggttgc tggagcagtt cgatgaatcg cgtgaagcgg ctggcgacgg cgagagggga
     6001 aagcggtaca cgtggaggcg cttgcaggtg tccgcccaag agccgcgcga gatcctcgtg
     6061 cacgtacgcg ccgtttcggc ctgcatcggt gtcctggacc gtatggtgcg catcggctga
     6121 tcgaccgaag gggcgcgcgg acgcttcagc cgccactgag tgcctgtacg aggacgagcg
     6181 tgtcgtccgc gcgcatcgga tgggcaggat cgaagatctg tccgccgtcg gcgaagaagc
     6241 ggacgtggcg ccgcagccgg ccttgctcgt cgatgacgcg gaagcggatg cccggatagc
     6301 gacgatcgag gtcggtcagc accgcatcca gggtggcgcc ctcggcatcg acctcgcgca
     6361 cgccggtgta ggacagcagc ggcgttggaa tcagcacctt catgtcgcgg cggcgctcgg
     6421 atggccggtc tcgaccgcga ggatctccgg caggtgccgt gcgatgcact gccagtgctc
     6481 accctcgtcg gcgctcgtcc acagctcgcc gctggtggtg ccgaagtaga gcccgagcgg
     6541 gtccagccca tcggcgcaca tcgactggcg cttgaccgtc caccacgcct gctgcggcgg
     6601 caggccgcgg tccaggcgct gccagctcgc cccgccgtcg cgggtgccat acaccgccgg
     6661 gcggccgccc gggctcgtgc gcggccagac gtcgg
//
LOCUS       DAOQNN010000157         6682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6330_length_6682_cov_10.574468, whole genome shotgun sequence.
ACCESSION   DAOQNN010000157 DAOQNN010000000
VERSION     DAOQNN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6330_length_6682_cov_10.574468"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            491..1570
                     /locus_tag="PKV97_09565"
     CDS             491..1570
                     /locus_tag="PKV97_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB06162.1"
                     /translation="MLAGLQRRGLDGAPLLARAGIDLAETDTRIPVERYALLYNLCVE
                     ALEDEAFGLLPEPMRPGSFEFLCRALLGAPTLGEALLRAIRFLRIVLPAFRIELRVDG
                     ARAELLLDDGGSLGPGLDAPARVFAYEWLLRLLHGVASWFVGRGLALDAVAFPYARPA
                     HADDYALVYTEHSSFDAPQLAARLQANLLALPLRRDEAALVGFLEGAPGKITTLYRRD
                     REMVFRVRDILRDALPQNLSLEEVAERLHVSPRTLHRRLEDEGSGFRNIKEATRRDIA
                     YARLAKTRQPIARIAAELGYADPSTFYRAFVAWSGMSPEQFRHRLAGNDGLPAASAGP
                     DRRPAPTPRVTAGRQRSREFGPTEA"
     gene            complement(1541..4519)
                     /locus_tag="PKV97_09570"
     CDS             complement(1541..4519)
                     /locus_tag="PKV97_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HNB06163.1"
                     /translation="MPAPPENLRVFVASPGDVAAERRLALEVINGMQRRWFARGKVTL
                     DTVEWDDDLARVPMAASATPEDSVIDYLGRPRDCELTVVILWSRIGTRLPNSFRRADG
                     SAFESGTVWELEDARDRGRDVWVYRCTRPLEITGDDLKAQRVDEKLAQYRAVEAYFVR
                     AFQNPDGSLKGGVNTFDTPTSFGKALGEHLEAFITRRIDALRGASPRAPAGGAGAGAK
                     PWLVREPVRDYHVVGRAELVDRVWRKLRDGVDTSLLFLPGVGKTTVAQELLRERDKIL
                     GHFDGVLWANLGRQHVDSAELRAWGEALGIPAERMNSFDDKELWLRALQDAIGDRCML
                     VVLDDVWRTEDARRFMELGPRCVFLSTTRSKTVAKELGAGMLVPELDPPQAFGLLCEV
                     APGAVEADPMAAKALLGTLQGLPLAIVLVGKVLRRAASEAHADPARLRRAYDEVAQAG
                     RRLGLLRPEDQRTLGEVIELSFGALRSDAARDAATALSIFRPKPNAFGRDIALAVGEA
                     DEQTLEDIGDIGLIEQVGKDVFTMHRIIAEYARTRLPLQRSQALHRKALAFYAEKLRG
                     SIESDPDAYLSWYRHEWAEWQETKDACLYHTAHSGDSVAGVLAFLRVFFDAFWWWGYY
                     QRFPFCERLVVEWRQRELDARARALIDQVAAFQDAYPAGPDKRAGGDWVRAESALLGI
                     RKALGLDGDGRHITGEDARRVRAFTDFFLAEARAYGRDERDEALRFYEAARSLFLDDG
                     VSWVAAWIEFYVAQYLLERGEFADARPHALRSLAEGAGEFGERAPLAGRDSELIANDY
                     RLLGDLALAADEVDAAARAHGRATLYAYVFQAIPEPVDTYTMDFYGEIVGRVAASIAR
                     CGSGDPRGRALALALHAFWRPWRTHCGIGDERDELNAALDAGDPGRLAAALFPPAPAV
                     DAFDFIDQAASYSRAVLAVVPSFVHELGPLDEFMAEVVRTLAGGVGADGTALAGHAPL
                     YRIDARKGRLQLCLGRTEFA"
     gene            complement(4520..4849)
                     /locus_tag="PKV97_09575"
     CDS             complement(4520..4849)
                     /locus_tag="PKV97_09575"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNB06164.1"
                     /translation="MVVIVFRTRLRAGVDEAELEGVGARMFQVAASMPGYVSYKDFAA
                     DDGEFVSIVEFDSLETLAAWRDHPEHREIQARGRSRYFSDYRVQVCTTVREYAFTLDG
                     GRVEGAL"
     gene            5083..>6682
                     /locus_tag="PKV97_09580"
     CDS             5083..>6682
                     /locus_tag="PKV97_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HNB06165.1"
                     /translation="MARSPSPQAEPAAFTPEQLRRHDLLLAGLDLLDQAIAVFDTTPK
                     LVTWNKALVRLLDFPEALVRVGTPFEAFVRFNAERGEYGEGDIETLVAKRMAAARAFL
                     PHYVERARPNGTVLAVRGVPIPNLGFVSLWTDITEQRRYEALIQQQNAQLEARVRERT
                     AELEQANSEIDQIAGALRRSEHRLQLIIDSIPALIAYVDGGEIYRFANRGYAEWFGYP
                     KDEIVGRSIREVVGEDAYAQIRSQVERARTGERVCYEYSRVGTDGRTLHARSVVVPEI
                     GTDGRTVVGFFVMTTDITEQKANQAALVQAQKMEAVGQLTGGLAHDFNNLLTIIIGNL
                     SALQQRIGAGPGAEFVDPSLQAARRGAELIRRLLTFSRRQSLAPTAVEVGALVHNMTH
                     LLARTLGETIRIHLRLPGEPVHALVDPHQLENAILNLAINARDAMPVGGELTIGVSRR
                     QLDEALAGVVEVAAGDYVQIDVGDTGKGIEPAVLQRVFEPFFTTKDFGSGSGLGLSMV
                     YGFVRQSGGNIRILSTPGKGTNVRF"
BASE COUNT         1063 a         2442 c         2196 g          981 t
ORIGIN      
        1 acgcgtcatg gccgtcgaac agcgcggcag cggtgacgaa acgcaccttg ttcttcggct
       61 tgtagggcga cagcttgtgc gcaacgctca gatcggtcat gtcgttcgtc tccaggtggg
      121 tgtgaacatc cctgcaatat tagaagggtc ttcacgcctt tgccattgcg cttgccgacg
      181 aggatcgtgc aaaatccgcc aaatcgagca accgatggcg cgctcggcgg cttgccggtt
      241 gccgctttcc cgtgggagcg acgcaagtcg cgattgcagc gctaccgcgg acgatgacga
      301 cggcaggcac cggatgcgga aatcgcaacg tgtgccgcgc ctgcacagcg cagcgtcgcg
      361 aagcctctgc gcagcgccgg ctcgatccct ccacccaagc caggagcccg ccctgtcgac
      421 tcccagcctt tccgcttcag cgatcccccg ccgtagccgc gccacggtcg cgcccggctt
      481 cgtcaccggc atgctggccg ggctgcagcg gcgcggactc gatggggcgc cgctgctcgc
      541 acgcgccggg atcgaccttg cagaaaccga cacgcgcatc ccagtcgagc gctacgccct
      601 gctctacaac ctgtgcgtgg aggcacttga agacgaggcc ttcggcctgc tcccggagcc
      661 gatgcgcccg ggcagcttcg agttcctctg ccgcgcactg ctcggcgcgc ccacgctggg
      721 cgaggccctg ctgcgcgcga tccgcttcct gcgcatcgtg ttgcccgcct tccgcattga
      781 gctgcgggtg gacggcgcgc gcgccgagtt gctgctggac gacggcggca gcctcggccc
      841 cgggctcgac gccccggcgc gcgtgttcgc ctacgaatgg ctgctgcgcc tgctgcacgg
      901 cgtggcgagc tggttcgtcg gccgcggcct ggcgctcgac gcggtcgcct tcccttacgc
      961 acgcccggcc catgcggacg actacgcgct cgtctacacc gagcattcga gcttcgacgc
     1021 gccgcagctg gccgcccgcc tgcaggccaa cctgctggcg ctgccgctgc gccgcgacga
     1081 ggcggcgctg gtcggcttcc tcgagggcgc accgggaaag atcaccaccc tctaccggcg
     1141 cgaccgcgag atggtcttcc gcgtgcgcga catcctgcgc gacgcgctgc cgcagaacct
     1201 ttcgctcgaa gaggtcgccg agcgcctgca cgtgtcgccg cgcaccctgc accggcggct
     1261 ggaggatgag ggctcgggat tccgcaacat caaggaggcc acccgccgcg acatcgccta
     1321 tgcgcgcctg gccaagaccc gccagcccat cgcccgcatc gcggccgagc tcggctacgc
     1381 cgacccgtcc accttctacc gcgccttcgt cgcctggagc ggcatgtcgc cggagcagtt
     1441 ccggcaccgg ctggcgggca acgacggtct tcccgcggcg tctgccggtc cggacaggcg
     1501 ccctgcccca accccccgtg tcaccgccgg acggcagcga tcacgcgaat tcggtccgac
     1561 cgaggcatag ctggagtcgc cccttgcggg cgtcgatgcg atacaggggc gcatgcccag
     1621 caagcgctgt gccgtccgct cccactccgc ccgccagggt cctgaccacc tccgccatga
     1681 actcgtcgag cgggcccagc tcgtgcacga acgagggcac caccgcgagc acggcgcgac
     1741 tgtaggatgc cgcctggtcg atgaagtcga acgcgtccac cgccggggcg ggcgggaaca
     1801 gcgcagcggc caggcgcccc gggtcgccgg catccagcgc tgcattcagt tcgtcgcgtt
     1861 cgtcgccgat gccgcaatgc gtccgccaag gccgccagaa ggcgtggagc gcaagcgcca
     1921 gcgcgcggcc gcgcgggtca cccgatccgc agcgcgcgat cgatgcggcg acacgaccga
     1981 cgatctcgcc atagaagtcc atcgtgtagg tgtccaccgg ctcggggatc gcctggaaga
     2041 cgtaggcgta gagcgtggcc cgaccatggg cacgggcagc cgcgtcgacc tcgtcggcag
     2101 ccagcgcgag atcgccgagc aggcgatagt cgttggcgat gagttcggag tcgcgccccg
     2161 cgagcggcgc ccgctcgcca aactcgccgg cgccctcggc cagggaacgc agggcatgcg
     2221 ggcgggcatc ggcgaactcg ccgcgctcga gcaggtattg ggccacatag aactcgatcc
     2281 aggccgcgac ccaggacacg ccgtcgtcga gaaacagcga gcgcgccgcc tcgtagaagc
     2341 gcaaggcctc gtcgcgctcg tcacgcccat aggcccgtgc ttcggcaagg aagaaatcgg
     2401 tgaaggcacg cacccgtcga gcgtcctcgc cggtgatgtg gcggccatcg ccatcgaggc
     2461 cgagcgcctt gcgtatcccg agcagcgcgc tctcggcgcg cacccagtcg ccgccggcac
     2521 gcttgtcggg gcccgccggg taggcatcct ggaacgctgc cacctgatcg atcaaggcac
     2581 gcgcccgcgc gtcgagttcg cgctgcctcc actcgacgac cagccgctcg cagaacggga
     2641 agcgctggta gtagccccac caccagaacg catcgaagaa cacgcgcagg aaagccagca
     2701 cgcccgccac cgagtcgccg ctgtgggcgg tgtggtacag gcaggcgtcc ttggtctcct
     2761 gccactccgc ccattcgtgg cgataccagc tcagataggc atccggatcg ctctcgatcg
     2821 agccccgcag cttctcggca tagaaggcga gcgccttgcg atgcagcgcc tgcgagcgct
     2881 gcaggggcaa gcgggtgcgc gcgtactcgg cgatgatgcg gtgcatcgtg aacacgtcct
     2941 tgcccacctg ctcgatcagc ccgatgtcgc cgatgtcctc cagtgtctgt tcgtcggcct
     3001 cccccacggc gagcgcgatg tcgcggccga aggcgttcgg cttgggacgg aagatggaca
     3061 gcgcggtcgc cgcgtcgcgc gccgcgtcgc tgcgcagcgc gccgaaggac agttcgatca
     3121 cctcccccag cgtgcgctgg tcttcaggac gcagcagccc gagccggcgg cccgcctgcg
     3181 ccacctcgtc gtaggcgcgg cgcaggcgtg cgggatcggc atgcgcttcg ctcgccgccc
     3241 ggcgcaagac cttgcccacc agcacgattg ccagcggcag gccctgcagg gttccgagca
     3301 gggccttggc ggccatcgga tccgcctcga cggcgcccgg ggcgacctcg cacaggaggc
     3361 cgaaggcctg cggcgggtcc agctccggga ccagcatgcc ggcgccgagc tccttcgcca
     3421 ccgtcttcga gcgcgtggtg gacaggaaga cacagcgcgg acccagctcc atgaaacgcc
     3481 ttgcatcctc ggtgcgccac acgtcgtcga gcaccacgag catgcagcgg tcgccgatgg
     3541 cgtcctgcag cgcacgcagc caaagctcct tgtcgtcgaa actgttcatg cgctcggccg
     3601 ggatgccgag cgcttcgccc catgcacgca gctccgcgct gtcgacgtgt tgccggccga
     3661 gattggccca caagacgccg tcgaaatgcc cgaggatctt gtcgcgctcg cgcagcagtt
     3721 cctgcgcgac cgtggtcttg ccgacgccgg gcaggaacag caggctggtg tcgacgccgt
     3781 cccgcagctt gcgccacaca cggtcgacga gctccgcgcg ccccaccacg tggtaatcgc
     3841 gcacgggctc gcgcaccagc cagggcttcg cccctgcgcc cgccccgccc gcgggcgcgc
     3901 gcggactcgc gccccgcagg gcatcgatgc gccgcgtgat gaaggcctcg aggtgctctc
     3961 cgagcgcctt cccgaagctg gtcggcgtat cgaaggtgtt cacgccgccc ttgagcgaac
     4021 catcgggatt ctggaatgcc cgcacgaagt aagcttccac cgccctgtac tgcgcgagct
     4081 tttcgtcgac gcgttgggcc ttgaggtcat cccccgtgat ctcgagcgga cgggtgcagc
     4141 ggtagaccca cacatcgcgg ccgcggtccc ttgcgtcctc gagttcccag acggttcccg
     4201 actcgaaggc actgccgtcg gcacggcgga acgagttcgg caggcgcgtg ccgatgcggg
     4261 accacaggat gaccaccgtg agctcgcaat cccgcggccg gcccaggtag tcgatgaccg
     4321 aatcctccgg cgtcgccgac gccgccatcg gcacccgggc aaggtcgtcg tcccattcga
     4381 cggtgtcgag cgtcaccttt ccgcgcgcga accagcgccg ctgcatgccg ttgatgacct
     4441 cgagcgccag cctgcgctcg gccgcgacat cacctggcga ggcgacgaag acgcgcagat
     4501 tctcgggcgg cgccggcatt cacaaagccc cttcgacccg cccgccgtcc agcgtgaagg
     4561 cgtactcgcg cacggtcgtg cacacctgta cccggtaatc gctgaagtac cgactgcggc
     4621 cccgcgcctg gatctcccgg tgctcggggt ggtcgcgcca tgcggcgagg gtctccagcg
     4681 agtcgaactc gacgatggaa acgaactcgc catcgtccgc cgcgaaatcc ttgtacgaga
     4741 cgtagcccgg catggatgcg gccacctgga acatgcgggc gcccacacct tcgagttccg
     4801 cctcgtcgac gcccgcgcgc aggcgcgtgc ggaatacgat cacgaccatg acgtcctccc
     4861 gcgcttccgg ttcgcatccg cgcaccgccc gccggcgaat cagatgcatt cccatgatag
     4921 tcaagctttc gcgcctgcgc cgcgccgagg agaggccgcc cggcacaagg tcgcggagcc
     4981 ttgtcgccca ccgcggtgaa cgccacaggc atcgcgacgc gagatgtgca aacatcgcgc
     5041 tcatgatcct cgaaactcgc cacaccggag actgctgcga gcatggcaag atcgccctcc
     5101 cctcaggctg aacccgccgc cttcactccg gagcagctcc ggcggcacga cctgctgctg
     5161 gccggcctcg atctgctcga ccaggccatc gcggtgttcg acaccacgcc caagctcgtc
     5221 acctggaaca aggccctggt gcgcctgctc gatttccctg aggcgctggt gcgcgtcggc
     5281 accccgttcg aggccttcgt gcgcttcaac gccgaacgcg gtgaatacgg cgaaggcgac
     5341 atcgaaaccc tggtcgcgaa gcgcatggcg gcggcacggg ccttcctgcc gcactacgtc
     5401 gaacgcgcac ggccgaacgg cacggtgctg gcggtacgcg gggtgccgat tcccaacctc
     5461 ggtttcgtgt cgctatggac cgacatcacc gagcagcgtc gctacgaggc gctgatccag
     5521 cagcagaacg cgcagctcga agcgcgcgtg cgcgagcgca cggcggagct ggagcaggcc
     5581 aacagcgaga tcgaccagat cgccggcgcg ctgcgtcgca gcgaacaccg cctgcaattg
     5641 atcatcgact ccatccccgc gctgatcgcg tatgtggacg gcggcgagat ctaccgcttc
     5701 gccaaccgcg gctacgccga atggttcggc tatccgaagg acgagatcgt cggccgcagc
     5761 attcgcgagg tggtcggcga ggacgcctac gcccagatcc gctcgcaggt cgagcgcgcg
     5821 cgcaccggcg agcgtgtgtg ctacgaatac agtcgcgtcg ggaccgacgg tcgcacgctg
     5881 catgcacgca gcgtggtggt gcccgagatc ggcaccgacg ggcgcacggt ggtgggcttc
     5941 ttcgtcatga ccaccgacat cacggaacag aaggccaacc aggccgcgct ggtgcaggcg
     6001 cagaagatgg aggcggtggg ccagctcacc ggcgggctgg cgcacgactt caacaacctg
     6061 ctcaccatca tcatcggcaa cctctccgca ctgcagcagc gcatcggcgc cgggccgggg
     6121 gcggagttcg tcgatccctc gctgcaggcg gcacggcgcg gcgccgagct gatccgccgc
     6181 ctgctcacct tctcgcgccg gcagtcgctc gcccccaccg cggtcgaggt cggcgcgctc
     6241 gtgcacaaca tgacgcacct gctcgcgcgc accctgggcg agaccatccg catccatctg
     6301 cgcctgcccg gcgagccggt gcacgcgctg gtcgacccgc accagctcga gaacgcgatc
     6361 ctcaacctcg cgatcaacgc acgcgacgcc atgcccgtgg gcggcgagct gaccatcggc
     6421 gtgagtcgcc gccagctcga cgaggccctc gcaggggtgg tcgaggtggc ggccggcgac
     6481 tacgtgcaga tcgacgtcgg cgataccggc aagggcatcg agccagccgt gctgcagcgc
     6541 gtgttcgagc ccttcttcac caccaaggac ttcggcagcg gcagcgggct gggcctgtcg
     6601 atggtgtatg gcttcgtacg tcagtcgggc ggcaacatcc gcatcctcag cacgccgggc
     6661 aagggcacca acgtgcgctt ca
//
LOCUS       DAOQNN010000158         6636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6411_length_6636_cov_8.863850, whole genome shotgun sequence.
ACCESSION   DAOQNN010000158 DAOQNN010000000
VERSION     DAOQNN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6411_length_6636_cov_8.863850"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /gene="petA"
                     /locus_tag="PKV97_09585"
     CDS             <1..413
                     /gene="petA"
                     /locus_tag="PKV97_09585"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNB06166.1"
                     /translation="TVEWRGKPVWILRRTPEMLATLEHNKARLADPVSGEPQQPEYAT
                     NRQRSIKPEYLVVIGICTHLGCSPSEKFAIGTTEGMTADWPGGFLCPCHGSIFDLAGR
                     VYLSQPAPTNLEIPPHKWLSDGLVLIGEDDHGMA"
     gene            618..1634
                     /locus_tag="PKV97_09590"
     CDS             618..1634
                     /locus_tag="PKV97_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585314.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNB06167.1"
                     /translation="MNPVPALVSPSVHAPVLDPVDEPHAVSPFGERRYNAWNDHVKRR
                     WGGRVQKVSVAAGFTCPNRDGTLGSGGCTFCNNAGFTPGYLDRRDPIGEQIDAGLRFL
                     DRRYPGTARFIAYFQTYSNTYGEFARLRESYEAALAHPRICGLAIGTRPDCLPDAVLD
                     YLAQLAREHIVELEIGVESCDDEVLRRINRGHDFACSEEAIRRAAARGLGVTAHLILG
                     LPGETRAGMLEAARRLSALPVAALKLHQLQLVRGTAMARDWERDPASVPLLAEDEYIA
                     LLADFVERLAPTILLQRLGSEVPPALKLAPQWTIRLSELAPRLSAELARRESWQGRRY
                     QPAC"
     gene            complement(1973..2431)
                     /locus_tag="PKV97_09595"
     CDS             complement(1973..2431)
                     /locus_tag="PKV97_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB06168.1"
                     /translation="MSRQPLARILLLALPAVLLLACSSEPPDTHPDKPVTQRRDAFKA
                     MLRSTEPMNTMLKDRRFDADQFASLAEQLAAKRDAPWKHFGPGSNYPPTKARPAVWEK
                     PAEFERRRSEFITATDRLLAAAAARSEEQARTALAAVQDSCKACHNDFRR"
     gene            2669..5314
                     /locus_tag="PKV97_09600"
     CDS             2669..5314
                     /locus_tag="PKV97_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNB06169.1"
                     /translation="MNSAVPTSAGPIPSDLPTSPAKSAPTLPATFAPTTGLPDLDAML
                     RGVARGDNIVWQVQALDDYRALVLPYAAAAQAQGRRLIYFRFAAHPALLGEDSAAEVF
                     TPRPEDGFDAFVDQVHSVIEAAGPETMYVFDCLSDLAEAWLSDRMLGNFFRLTCPRLF
                     DLDTVTYFGVLRHRHARAGMDVITETTQYLLDVFRCRDRLYIRPIKVQHRSAAAMNLI
                     HAWEPGDRFRAVADSGTVAEILAGSGWQGLEDEQIRSHWQRLFDQARTFAALRAAGAP
                     ELAGEAAAFARLAGLLFPDEAGLQELIARYLNLDDLLAVRARMIGIGTIGGKAAGMLI
                     ARAIVRRDAPALHARLEAHDSFHVGSEVFDTFFVLNGLWWVRRRQRDAAGFLRGLDGE
                     DGARARILRGRFPESTLRQFEGMLDYFGEWPFIVRSSSRLEDRYGNAFAGQYDSVFCA
                     NRGTREERLAALLDAVRQVYASTLSESALRYRQRRGLLDEHEQMALLIMRVSGTAGGR
                     WFNPHAAGVGLSVNPYPWNPRIDMRAGVVRLVAGLGTRAVDRADDDYTRLIALDAPEL
                     RPETSFGAIARHAQRRMDSLDLERGEVVSGPFAELVRGHADFPHALFTTREAEDRPAF
                     LTFDGLIAHTDFVADLRTLLRALHAAYAHPVEIEFALNFVPDGGYRINLLQCRPMQVR
                     SVEGVQGVEPPADAPRLVEACGAVIGPSRVIRPERIVLVRPAAFAALDQARRLAVTRV
                     LGRLNALADERCVLMLGPGRWGSKDPFLGIPVLFSEINHVAALCEIVAMHEHLVPDVS
                     LGTHFLNELIEADLLYFALFPRMAGNVLDEAAILRWPNRLGTLLPDDADWSTVVHVAE
                     PAQAPTLYADAPGQRVVVVQDTVAA"
     gene            5385..5840
                     /locus_tag="PKV97_09605"
     CDS             5385..5840
                     /locus_tag="PKV97_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06170.1"
                     /translation="MRLAFLSAAIAALCAAPVPVLAADDTRPQPKLQASADELPRPAD
                     EKLRAGMLQLRETVAARVSDPAMDEAAQVALAGEILRQVAMISEGRDFHSRPGRHLQW
                     LLGDISDGAGLMRDAPRLQAKRLGLLRVAETLNFYGREYDHPGWEPIRP"
     gene            5866..>6636
                     /locus_tag="PKV97_09610"
     CDS             5866..>6636
                     /locus_tag="PKV97_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNB06171.1"
                     /translation="MKPALRILAGPAARAQLRERGLAPADVLAIPGAAGGPKGLILNP
                     LDRFVFGHWLAGAGAPVHLLGASIGAWRMASACLPDADAELAEMARHYIEESYLDAEE
                     VQKARASGRPVRQPGAAAVSRAFRARLASHLGGRSDELLHHPRYRLHLFTSRGVHPLL
                     RREGRLRTLVGYAGAYAANLRSRARMGEWLERVVFSDPRDALPFALDDYAGRICALGP
                     HNAFEALVASCSIPFWLQAVPDIPGAPPGAYWDGGITDY"
BASE COUNT          899 a         2397 c         2317 g         1023 t
ORIGIN      
        1 tgaccgtgga gtggcgtggc aagccggtgt ggatcctgcg ccgcacgccc gagatgctcg
       61 ccacgctcga gcacaataag gcccgcctcg ctgaccctgt ctcgggcgaa ccgcagcagc
      121 ccgagtacgc caccaaccgg cagcgctcga tcaagccgga atacctcgtt gtgatcggca
      181 tctgcacgca cctgggctgc tcgccgtccg agaagttcgc catcggcacc accgagggca
      241 tgaccgccga ctggcccggc ggcttcctgt gcccctgcca cggctcgatc ttcgacctcg
      301 ccggccgcgt ctatctcagc cagccggccc ccaccaacct cgagatcccg ccgcacaagt
      361 ggctctccga cggcctcgtg ctgatcggcg aggacgacca cggcatggca tgaacggggc
      421 tgccgctcct gcagggcttc cgaagcgcgg cacttccgcg caagccattc tgctccgcgg
      481 ggcgatttct ccagcggcct gcgtttgcgc acggtcaagg gctgcgcggg cgatcggcgg
      541 cacactcgcg cctcggcaac ttcgccagcg gcatccggct gcgccgcttt caccgtccat
      601 ccactttcgc tccccgcatg aacccggttc ccgcactcgt ctctccctcc gttcacgctc
      661 cggtgctgga tccggtcgat gagccgcacg cggtttcgcc cttcggcgag cgccgctaca
      721 acgcctggaa cgaccacgtg aagcggcgct ggggtgggcg ggtgcagaag gtctcggtgg
      781 cggccggctt cacctgcccg aaccgcgacg gcaccctggg cagcggcggc tgcaccttct
      841 gcaacaacgc cggcttcacc cccggctacc tggatcgccg cgacccgatc ggcgagcaga
      901 tcgacgccgg gctgcgcttc ctcgaccggc gctatcccgg caccgcgcgc ttcatcgcct
      961 acttccagac ctacagcaac acctacggcg agttcgcgcg cctgcgcgaa agctacgagg
     1021 ccgcgctcgc gcacccgcgc atctgcgggc tggcgatcgg cacgcggccg gactgcctgc
     1081 ccgacgcggt gctggactac ctggcccagc tcgcccgcga gcacatcgtg gagctcgaga
     1141 tcggcgtgga gtcctgcgac gacgaagtcc tgcgccggat caaccgcggc cacgatttcg
     1201 cctgcagcga ggaggcgatc cggcgtgccg cggcgcgcgg cctgggggtg accgcccacc
     1261 tgatcctcgg cctgcccggc gagacgcgcg ccggcatgct ggaagccgcg cgccgcctgt
     1321 ccgcgctccc ggtggcggcg ctcaagctgc atcagctgca gctggtaagg ggcaccgcga
     1381 tggcacgcga ctgggagcgc gatcccgcga gcgtaccctt gctcgccgag gacgagtaca
     1441 tcgcgctgct ggccgacttc gtcgagcggc ttgcgcccac gatcctgctc cagcgcctgg
     1501 gcagcgaggt tcccccggcg ttgaagctgg cgccgcagtg gaccatccgc ctctccgaac
     1561 tcgccccccg gctgagtgca gagctggcgc ggagggaaag ctggcaaggc cgccgctacc
     1621 aacccgcttg ctgagtgtcg gcggcagggc caaggacgga agcgacgcga gtcgcgatcg
     1681 gagcgcatga ggttcgggaa cgcccggcgg gatctcgtac gctggaatcg cacttgcgcg
     1741 agaccgcggc gtctccggaa gcgcaccgtg ggagcgacgc gagtcgcgat cggagcgcat
     1801 gaggttcggc aacgctcggc gggatctcgt acgctggaat cgcacttgcg cgagaccgcg
     1861 gggtctccgg aagcgcgccg tgggagcgac gcgagtcgcg atcgggcgca ggagatgcga
     1921 cgacgcccgg tgggaccgcg ggttccgcaa tcgcgcctgc agtcttgccg cctcagcgcc
     1981 tgaagtcgtt gtggcaggcc ttgcagctgt cctgaacggc tgcgagggcg gtgcgcgcct
     2041 gttcctccga gcgcgccgca gcggcggcga ggaggcggtc ggtggcggtg atgaactcgc
     2101 tgcgacggcg ctcgaactcg gcgggctttt cccagacggc cgggcgggcc ttggtggggg
     2161 ggtaattgct gccggggccg aagtgcttcc acggcgcgtc gcgcttcgcg gcgagctgct
     2221 cggcgaggct ggcgaactgg tcggcgtcga aacgccggtc cttgagcatg gtgttcatgg
     2281 gctcggtgct gcgcagcatg gccttgaagg cgtcgcggcg ctgggtgacc ggcttgtccg
     2341 ggtgggtatc gggcggctcc gacgaacagg cgagcagcag gacggcgggc agggcgagga
     2401 gcaggatgcg ggcgaggggc tgtcgggaca tttcgggcgt gaggagtcgg gagtgatgcg
     2461 tcgggtgccg gtctaggctc ttgcggaact cgaaggcgag gcgggaaggg tcggattccc
     2521 ggcacgaggg cgcgcggccg ccggccgatg gtgcagcagc gcaatcgttc gacggttggc
     2581 aaggcaagaa gaatgccacg cggcgggaaa gttcccgcgg ggcttgcgcg cataatcgcg
     2641 ctcgcaggcc ttgtccgagg agctcctgat gaattctgcc gttccgacgt cggccggacc
     2701 catcccgtcc gatcttccca cgtctcccgc caagtccgct cccacgctcc ccgccacctt
     2761 cgccccgacc accggcctgc ccgacctcga cgccatgctg cgtggcgtgg cgcgcggcga
     2821 caacatcgtc tggcaggtgc aggcgctgga cgactaccgc gccctggtgc tgccctatgc
     2881 ggccgcggcg caggcgcagg gacggcggct gatctatttc cgctttgccg cgcatcctgc
     2941 cctgctcgga gaagacagcg ccgccgaggt gttcaccccg cgtcccgagg acggcttcga
     3001 cgccttcgtc gaccaggtgc attcggtgat cgaggccgcg ggaccggaga ccatgtacgt
     3061 cttcgactgc ctttctgacc tcgccgaagc ctggctgtcc gaccgcatgc tcggcaactt
     3121 cttccgcctc acctgcccgc ggctcttcga cctcgacacg gtgacctact tcggcgtgct
     3181 gcgccatcga catgcgcgcg cgggcatgga cgtgatcacc gagacgacgc agtacctgct
     3241 cgacgtgttc cgctgccgcg atcggctcta catccggccg atcaaggtgc agcaccgctc
     3301 ggccgcggcg atgaacctga tccacgcctg ggagccgggc gatcgcttcc gcgcggtcgc
     3361 cgacagcggc acggtggccg agatcctcgc cggctcgggc tggcaggggc tggaggacga
     3421 acagatccgc agccactggc agcgcctctt cgatcaggcg cgcaccttcg ccgccctgcg
     3481 cgcggccggg gcgcccgagc tggccggcga ggccgccgcc ttcgcccgcc tggcgggcct
     3541 gctgtttccc gacgaggcgg gcctgcagga gctgatcgcg cgctacctga acctcgacga
     3601 cctgctcgcg gtgcgcgcgc gcatgatcgg catcggcacc atcggcggca aggccgcggg
     3661 catgctgatc gcgcgcgcga tcgtgcgccg cgacgcgcct gcgctgcacg cccgcctcga
     3721 ggcgcacgac tccttccatg tcggcagcga ggtcttcgac accttcttcg tgctcaacgg
     3781 gctgtggtgg gtgcgccgcc gccaacgcga cgccgcgggc ttcctgcgcg ggctcgacgg
     3841 cgaggacggc gcgcgtgcgc gcatcctgcg cggccgcttc cccgaatcca ccctgcgcca
     3901 gttcgagggc atgctggatt attttggcga gtggcctttc atcgtgcgct ccagttcgcg
     3961 cctggaggac cgctacggca acgccttcgc cggccagtac gacagcgtgt tctgcgccaa
     4021 ccgcggcacc cgcgaggagc gcctcgccgc gctgctcgac gccgtgcgcc aggtgtatgc
     4081 gagcacgctg tcggagtcgg cgctgcgcta ccgccagcgc cgcggcctgc tcgacgagca
     4141 cgagcagatg gcgctgctga tcatgcgcgt ctcgggcacc gccggcgggc gctggttcaa
     4201 cccgcacgcg gccggcgtcg ggctgtcggt caatccctat ccgtggaacc cgcgcatcga
     4261 catgagggcg ggcgtggtgc gcctggtcgc cggtctgggc acacgtgcgg tggatcgcgc
     4321 cgacgacgac tatacccgcc tgatcgcgct cgatgcgccc gagctgcgcc cggagaccag
     4381 cttcggtgcg atcgcgcgcc atgcccagcg ccgcatggac agcctcgacc tggagcgcgg
     4441 cgaggtggtc tcgggcccct tcgccgagct ggtgcgcggc catgcggatt ttccgcacgc
     4501 gctgttcacg acgcgcgagg ccgaggaccg tccggccttt ctgaccttcg acgggctgat
     4561 cgcccacacc gatttcgtcg ccgacctgcg cacgctgctg cgcgcgctgc acgcggccta
     4621 cgcgcatccg gtggagatcg agttcgcgct caatttcgtt cccgacggcg gctaccgcat
     4681 caacctgctg cagtgccggc cgatgcaggt gcgcagcgtc gagggcgtgc agggcgtgga
     4741 gccgccggcc gacgcgccgc gcctggtgga agcgtgcggt gcagtgatcg gaccgagccg
     4801 ggtgattcgc cccgagcgca tcgtgctggt gcgcccggcc gccttcgccg cgctcgacca
     4861 ggcccgccgc ctggcggtga cgcgcgtgct cggccgcctc aacgcgcttg ccgacgaacg
     4921 ttgcgtgctg atgctcggcc ccgggcgctg gggctcgaag gacccctttc tcggcatccc
     4981 ggtgctgttc tccgagatca atcacgtcgc cgcgctgtgc gagatcgtcg ccatgcacga
     5041 gcacctggtg cccgacgtct cgctcggcac ccacttcctc aacgagctga tcgaggccga
     5101 cctgctgtac ttcgcgctgt tcccccggat ggcgggcaac gtactcgacg aggcggcgat
     5161 cctgcgttgg cccaaccggc tcggcacgct gctgccggac gacgcggact ggagcacggt
     5221 ggtgcacgtg gccgagcccg cgcaggcacc cacgctgtac gccgatgcgc ccgggcaacg
     5281 tgtcgtcgtg gtgcaggaca cggtggcggc ttgattcagt tcaagcgctt tcaggggccg
     5341 cccgggtagg atggcgaccg ttcgcatcca accggagatc gtgcatgcgc ctcgccttcc
     5401 tgagcgccgc catcgcggcg ctgtgtgccg cccctgttcc cgttctcgcc gccgacgaca
     5461 cacgcccgca acccaagctg caggcatcgg ccgacgagtt gccgcgcccc gccgacgaga
     5521 agctgcgcgc cggcatgctg caactgcgcg agaccgtcgc cgcacgcgtg tccgacccgg
     5581 ccatggacga ggctgcgcag gtcgcgctcg ccggcgagat cctgcgccag gtcgccatga
     5641 tcagcgaggg gcgcgacttc cacagccgtc ccggccgcca cctgcaatgg ctgctcggcg
     5701 acatcagcga tggcgccggg ctcatgcgcg atgcgccgcg cctgcaagcc aagcggctcg
     5761 gcctgctgcg ggtcgccgag acgctgaact tctacggtcg cgagtacgac cacccggggt
     5821 gggagccgat caggccgtga ggggccgcgc ggaggcgacg gcgcgatgaa gccggcgctg
     5881 cgcatcctgg ctggtccggc ggcgcgtgcg cagttgcgcg agcgcggttt ggcgcccgcc
     5941 gacgtccttg cgatccccgg cgcggccggc ggccccaagg gactcatcct caatccgctc
     6001 gatcgcttcg tcttcggcca ttggctggcg ggcgcgggtg cgccggtgca cctgctcggc
     6061 gcctcgatcg gcgcctggcg catggccagc gcatgcctgc ccgatgcgga cgccgagctc
     6121 gccgagatgg cgcgtcatta catcgaggag agctatctcg acgccgaaga ggtgcagaag
     6181 gcgcgcgcca gcggccgccc ggtgcgccaa cccggcgcgg cggcggtcag ccgcgccttc
     6241 cgcgcgcgcc tcgccagcca tctgggcggg cgcagcgacg agctgctgca ccacccccga
     6301 tacaggctgc acctcttcac cagccgcggc gtgcatcccc tgctgcggcg cgagggtcgg
     6361 ctgcgcacgc tcgtcggcta tgcgggcgcc tacgccgcca acctgcgcag ccgcgcccgc
     6421 atgggcgaat ggctcgagcg cgtggtgttc tcggatccgc gcgacgcact gccctttgcc
     6481 ctggacgact acgccgggcg catctgcgcg ctcggtccgc acaacgcctt cgaggccttg
     6541 gtggcgagct gctcgatccc gttctggctg caggcggtgc ccgacattcc cggcgccccg
     6601 ccgggtgcct actgggatgg cggcatcacc gactac
//
LOCUS       DAOQNN010000159         6631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6422_length_6631_cov_8.054745, whole genome shotgun sequence.
ACCESSION   DAOQNN010000159 DAOQNN010000000
VERSION     DAOQNN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6422_length_6631_cov_8.054745"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKV97_09615"
     CDS             <1..275
                     /locus_tag="PKV97_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB06172.1"
                     /translation="GGIVSMTLPIARELARFGIRVMTIAPGLFITPMMRGLPPEVQKS
                     LGENTPFPPRLGEPEEFAKLALSIIDNVMLNGETIRLDGAVRLAAR"
     gene            289..708
                     /locus_tag="PKV97_09620"
     CDS             289..708
                     /locus_tag="PKV97_09620"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNB06173.1"
                     /translation="MARRHLHRMRIAFSDCDPAQIVFFANYFKWFDTAFREFCTACGV
                     PSWRETTATRGIIGAPLVDAQAKFRNSATYGEDIEIESWVEDWGGKSFVMRHVARRGD
                     TVLCEGREVRVFAVQDPEDPLRIKAIPIPEDLRAACS"
     gene            746..2533
                     /locus_tag="PKV97_09625"
     CDS             746..2533
                     /locus_tag="PKV97_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNB06174.1"
                     /translation="MYDYQPPLRDMQFILAELAGLDDIARLPGCEEASADVIGAVLEE
                     AGRFAAGVLAPLNRAGDEQGCRLLEGGRVATPEGWQQAYAQFCAAGWLGLSLPVEFGG
                     QGLPKLVSTPVMEMWNAANMAFTMLPILNQGQAEALRIAASAEQKRTWLPKVVSGEWG
                     STMNLTEPQAGSDLAATRTRAEPQADGSYKLFGQKIFISYGEHELTPNIVHLVLARLP
                     DAPAGVKGLSLFIVPKFLVEPDGSLGARNDVRCISIEHKMGIHGSPTCTLSFGEGGGA
                     IGWLVGEPNRGLEVMFVMMNEARFGVGVQGAALAERAYQMALGYARERVQGRDAVSGA
                     AGRPILCHPDVKRMLLSMRARVMAMRALLYTAAGWFDIAHHHPDAAVADKHRRYVDLL
                     MPVAKGWCTELGNDVCDEAIQVFGGMGFVEETGVAQHFRDARIITIYEGTTGIQANDL
                     VGRKILREGGATLRELIVELRASATALSAAGLAELGDGQGAAIDALESATDWILANGR
                     EQLGEVLAGAVPFLHLLGTVCGGWQLGRAALAARARQAAGEGDPVYLDGIVELARFYM
                     ATLGPQAAAHAATVREAGGALTRFDEAAF"
     gene            2591..4159
                     /locus_tag="PKV97_09630"
     CDS             2591..4159
                     /locus_tag="PKV97_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HNB06175.1"
                     /translation="MSKSKVIPAAEAGKLIKDGATLYCSGVGLAGFAEEVAISIRERF
                     EATGHPRDMTIWHACGLGNGKNKGIFHLTQPGMIKRIVGGHFGVGGPVLMQLIMDSRI
                     EAYNLPQGVLVVLPREMAAGRPGLITKVGLETFVDPRIEGGKLNSATSEEIVERVDFD
                     GQEWLFYRAPRIDVALIRGTTADENGNLTVEDEGIFNESISIAQAAKRNGGIVIAQVL
                     QVVQNGTLHPKQVKVPGILVDHVVVGRPENHMQTMGTQYNRAFAGDIRVPVGSLRRLP
                     LDERKIIARRAAVELGHGAIVNLGIGVPAGIASIAAEEGVQDLMALTLETGPIGGIPA
                     DGLDFAHASNADATIDPGYQFDFYDGGGLDVAFLGLAQTDVHGNVNVSKFSGRPVGCG
                     GFINITQNAKKVVFCGAFTAGGLEIRAADGRLEIVREGTSRKFLDHVEQITFSGHYAA
                     GVGQTVMYVTERAVFQLTREGGMELLEVAPGVDIERDILAHMDFVPRMERVREMDAGI
                     FSERWGRLYEILHS"
     gene            complement(4231..5328)
                     /locus_tag="PKV97_09635"
     CDS             complement(4231..5328)
                     /locus_tag="PKV97_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HNB06176.1"
                     /translation="MTALFQPFKLKDLTLRNRIAIPPMCQYSATDGVVNDWHRVHYPA
                     LARGGAGLLIVEATAVSPEGRITPGCLGLWNDTQAAALAPIAASIKAAGAVPGIQIGH
                     AGRKASANRPWEGDDHIAADDARGWETLSPSATAFGAHLPKLPREMTVDDIARVRADF
                     VAAAKRARAAGFEWLELHFAHGYLGQCFFSVHANQRSDAYGGSFENRSRFLIETVRAV
                     RAVWPENLPLTVRFGVIEYDGRDEETLAESIELARSFKREGLDFLNVSVGFSTLKARV
                     PWGPAFLAPVAERVRREAGLPVAAAWGIDNPAIADACVRDGQLDLVMVGRAHLANPHW
                     PMHAARALGVERPTWVLPAPYAHWLERYAGA"
     gene            complement(5562..>6631)
                     /locus_tag="PKV97_09640"
     CDS             complement(5562..>6631)
                     /locus_tag="PKV97_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HNB06177.1"
                     /translation="GGSPAPDLWMQDNADDVGAEPDASTNPMWISDDIWVRNVADGLT
                     NQDHQNPNGEQTNYVYVRVRNRGCAGAAAQSGTLKLYWAKASSSLSWPAPWDGSVASP
                     ALMGGLVGSQAVSVNGGDTEIVEFAWTPPDPSDYAAFGADKAHFCLLARIETSATAPF
                     GMSFAETANLYANVQNNNNIVWKNISIVDTDGDGARYADVVIGRFTRERRATRLLFRT
                     PKRRGFSLFDWGHLMVEFRGEALLEWVKEGVKGDGFERLQDGRLFITGAHAEVVGPPL
                     KPGSFGTVHVQFVPDGRRPTGAQVFELDLIELDAKGRAVGGQRLLLKTGRAPAKPCCR
                     REAGSFDGVNWTPDSTCRCGC"
BASE COUNT          996 a         2324 c         2367 g          944 t
ORIGIN      
        1 agggcggcat cgtgtcgatg acgctgccga tcgcgcgcga actggcgcgt ttcggcatcc
       61 gcgtgatgac gatcgcgccg ggcctgttca tcaccccgat gatgcgcggc ctgccgcccg
      121 aggtgcagaa gtcgctcggc gagaacacgc ccttcccgcc gcgcctcggc gagcccgagg
      181 agttcgccaa gctggcgctg agcatcatcg acaacgtgat gctcaacggc gagacgatcc
      241 ggctagacgg cgcggtgcgc ctggccgcgc gctgagacgg ggcccgccat ggcacgccgc
      301 cacctccacc gcatgcgcat cgccttcagc gactgcgacc ccgcgcagat cgtgttcttc
      361 gccaactact tcaagtggtt cgacaccgcc ttccgcgagt tctgcaccgc ctgcggcgtg
      421 ccgagctggc gcgagaccac ggctacgcgc ggcatcatcg gcgcgcccct ggtcgacgcg
      481 caggcgaagt tccgcaactc ggccacctat ggcgaggaca tcgagatcga gtcctgggtc
      541 gaggactggg gcggcaagag cttcgtgatg cgtcacgtcg cgcggcgcgg cgacaccgtg
      601 ctgtgcgagg gcagggaggt gcgcgtgttc gcggtccagg acccggagga tcccctgcgc
      661 atcaaggcaa ttccgatccc cgaggatctc cgcgccgcct gcagctgagc gcgcgagaac
      721 aagaacgaca gagaggagac tccggatgta cgactaccag ccccccttgc gggacatgca
      781 gttcatcctc gccgagctcg ccgggctcga cgacatcgcc cgcctgcccg gctgcgagga
      841 ggccagcgcc gacgtgatcg gcgccgtcct cgaggaagcc ggccgcttcg ccgccggcgt
      901 gctggcgccg ctgaaccgcg cgggcgacga gcagggctgc cgcttgctgg agggcggccg
      961 cgtcgccacc cccgagggct ggcagcaggc ctatgcgcag ttctgtgccg cgggctggct
     1021 cggcctgtcg ctgccggtcg agttcggcgg ccagggactg cccaagctgg tgtccacgcc
     1081 ggtgatggag atgtggaacg ccgccaacat ggccttcacc atgctgccca tcctcaacca
     1141 gggccaggcc gaggcgctgc ggatcgccgc cagcgcggag cagaagcgca cctggctgcc
     1201 caaggtggtg agcggcgagt ggggcagcac gatgaacctg accgagccgc aggcgggctc
     1261 cgacctcgcc gccacgcgca cccgcgccga accgcaggcc gacggcagct acaagctgtt
     1321 cgggcagaag atcttcatct cctacggcga gcacgagctc acgcccaaca tcgtccacct
     1381 ggtgctcgcc cgcctgcccg acgcaccggc gggggtgaag gggctgtcgc tcttcatcgt
     1441 ccccaagttc ctcgtcgagc ccgatggcag cctgggtgcg cgcaacgacg tgcgctgcat
     1501 ctcgatcgaa cacaagatgg gcatccacgg cagcccgacc tgcacgctca gcttcggcga
     1561 gggcggcggc gcgatcggct ggctggtggg cgagcccaac cgcggcctcg aggtcatgtt
     1621 cgtgatgatg aacgaggcgc gcttcggcgt cggcgtgcag ggcgcggcgc tcgccgagcg
     1681 cgcctaccag atggcgctcg gctacgcccg cgagcgcgtg caggggcgcg acgcggtgag
     1741 cggggccgcg ggccggccca tcctgtgcca ccccgacgtc aagcgcatgc tgctgtcgat
     1801 gcgggcgcgg gtgatggcga tgcgtgcgtt gctctacacc gccgcgggct ggttcgacat
     1861 cgcccaccac caccccgatg ccgcggtggc ggacaagcac cgccgctacg tcgacctgct
     1921 gatgccggtg gccaagggct ggtgcaccga gctcggcaac gacgtctgcg acgaggccat
     1981 ccaggtcttc ggcggcatgg gcttcgtcga ggagaccgga gtcgcgcagc acttccgcga
     2041 cgcccgcatc atcaccatct acgagggcac caccggcatc caggccaacg atctggtggg
     2101 gcgcaagatc ctgcgcgagg gcggcgccac gctgcgcgag ctgatcgtcg agctgcgcgc
     2161 gagcgccacg gcgctctcgg ccgcggggct cgccgagctc ggcgacggcc agggcgcggc
     2221 gatcgacgcg ctcgagtcgg cgaccgactg gatcctcgcc aacggccgcg agcagcttgg
     2281 cgaggtgctc gccggcgcgg tgcccttcct gcacctgctc ggtaccgtgt gcggcggctg
     2341 gcagctcggg cgtgccgcgc tggcggcgcg cgcgcggcag gcggcgggcg agggcgaccc
     2401 ggtgtacctg gacggcatcg tcgaactcgc gcgcttctac atggcgaccc tgggcccgca
     2461 ggccgcggcc catgccgcca ccgtgcgcga ggccggcggt gcactgaccc gcttcgacga
     2521 agcggccttc tgatcaccgt ccgcccggca ggccgatgcc ttgccgggtg caaaaattgg
     2581 aactggacca atgtcgaaat cgaaagtcat tcccgccgcc gaggcgggca agctgatcaa
     2641 ggacggcgcc acgctgtatt gcagcggcgt gggcctggcg ggcttcgccg aggaggtcgc
     2701 gatctcgatc cgcgagcgct tcgaggccac cggtcatccg cgcgacatga cgatctggca
     2761 cgcctgcggg ctcggcaacg gcaagaacaa gggcatcttc cacctcaccc agccgggcat
     2821 gatcaagcgc atcgtcggcg gccacttcgg cgtgggcggg ccggtgctga tgcagctgat
     2881 catggacagc cgcatcgagg cctacaacct gccgcagggc gtgctggtgg tgctgccgcg
     2941 cgagatggcg gcaggccgcc ccgggctgat caccaaggtc ggcctggaga ccttcgtcga
     3001 tccgcgcatc gagggcggca agctcaactc ggccaccagc gaggagatcg tcgagcgcgt
     3061 cgacttcgac gggcaggagt ggctgttcta ccgcgcgccg cggatcgacg tcgcgctgat
     3121 ccgcggcacc accgccgacg agaacggcaa cctcacggtc gaggacgagg gcatcttcaa
     3181 cgagtcgatc tcgatcgccc aggccgcgaa gcgcaacggc ggcatcgtca tcgcgcaggt
     3241 gctgcaggtg gtgcagaacg gcaccctgca tccgaagcag gtcaaggtgc cgggcatcct
     3301 ggtggaccac gtcgtcgtcg gccgtcccga gaaccacatg cagaccatgg gcacgcagta
     3361 caaccgcgcc ttcgccggcg acatccgcgt gccggtcggt tcgctgcgcc ggctgccgct
     3421 cgacgaacgc aagatcatcg cccgccgcgc cgcggtcgag ctcggccacg gcgcgatcgt
     3481 gaacctcggc atcggcgtgc ccgccggcat cgcctcgatc gcagccgagg agggcgtgca
     3541 ggacctcatg gccttgaccc tggagaccgg cccgatcggc ggcatccccg ccgacgggct
     3601 cgacttcgcg cacgccagca acgccgacgc caccatcgac ccgggctacc agttcgactt
     3661 ctacgacggc ggcggcctcg acgtggcctt cctcgggctc gcccagaccg acgtccatgg
     3721 caacgtgaat gtctccaagt tcagcggccg gccggtgggc tgcgggggct tcatcaacat
     3781 cacccagaac gccaagaagg tggtgttctg cggcgccttc accgccggcg ggctggagat
     3841 ccgcgccgcc gacggccgcc tcgagatcgt ccgcgagggc acgagccgca agttcctcga
     3901 ccacgtcgag cagatcacct tcagcggcca ctacgccgcc ggggtggggc agacggtgat
     3961 gtacgtgacc gagcgcgcgg tgttccagct cacccgcgag ggcggcatgg aactgctcga
     4021 ggtggcgccc ggcgtcgaca tcgagcgcga catcctcgcc catatggact tcgtgccgcg
     4081 catggagcgc gtgcgcgaga tggacgccgg catcttcagc gagcgctggg gccggctgta
     4141 cgagatcctg cacagctgag acgcgggctc gaccccgggc ggccgcccgg ggtgaaaccc
     4201 ggccgcggcc gcctgcgccg ctgcttcgcc tcaagccccc gcgtagcgct ccagccagtg
     4261 cgcgtagggt gccggcagca cccaggtcgg gcgctcgacc cccagggcgc gcgcggcgtg
     4321 catcggccag tgcgggttgg cgaggtgggc gcggcccacc atcaccagat cgagctgccc
     4381 gtcgcgcacg caggcgtcgg cgatcgccgg gttgtcgatg ccccaggccg cggccaccgg
     4441 cagcccggcc tcgcggcgca cgcgttcggc gaccggggcg aggaaggcgg gaccccaggg
     4501 caccctggcc ttcagggtcg agaagccgac gctgacgttg agaaagtcca ggccctcgcg
     4561 cttgaagctg cgcgcgagct cgatcgactc ggcgagcgtc tcctcgtcgc ggccgtcgta
     4621 ttcgatgacg ccgaagcgca ccgtcagcgg caggttctcc ggccacaccg cgcgcaccgc
     4681 gcgcacggtc tcgatgagga agcggctgcg gttctcgaag ctgccgccgt aggcgtcgct
     4741 gcgctggttg gcgtgcaccg agaagaagca ctggccgagg tagccgtggg cgaagtgcag
     4801 ctccagccat tcgaagccgg ccgcgcgtgc gcgcttcgcg gcggcgacga agtccgcgcg
     4861 cacgcgggcg atgtcgtcca ccgtcatctc gcgcggcagc ttgggcaggt gggcgccgaa
     4921 ggccgtggcc gagggcgaca gggtctccca gccgcgcgca tcgtcggcgg cgatgtggtc
     4981 gtcgccctcc cacggccggt ttgcgctggc cttgcgaccg gcgtggccga tctggatccc
     5041 gggcaccgcg cctgcggcct tgatcgaggc cgcgatcggc gccagtgcgg cggcctgcgt
     5101 gtcgttccac aggccgaggc agcccggcgt gatgcggccc tcgggcgaca ccgcggtggc
     5161 ctcgacgatc agcagcccgg cgccaccgcg cgcgagcgcg gggtagtgca cgcggtgcca
     5221 gtcgttgacc acgccgtcgg tggctgagta ctggcacatc ggcgggatgg cgatacggtt
     5281 gcgcagggtg aggtccttga gcttgaaggg ctggaagagg gcggtcatga gcgatgtggt
     5341 tccgaagggg cgatgcaggc cgcgtggcgg ccggatgggg aaagatgagc gaagggattg
     5401 tgcggccgac gggccgaacg agggctggaa ggtagcggac gagcgctgcg cgcgtccact
     5461 cggcattgcc tcggctccaa aggcacaagc gaacggcccg gcgcctggcg acgctcgggc
     5521 cgttcgcact tcgcccgcgg acgaccgggg cggcaaaccc ctcagcagcc gcatcggcag
     5581 gtcgagtccg gcgtccagtt gacgccgtcg aagcttcccg cttcacgtcg gcagcaaggc
     5641 ttcgcgggcg cacggccggt cttcagcagc aggcgctgcc caccgacggc gcgccccttg
     5701 gcgtcgagct cgatcagatc gagctcgaag acctgggcgc cggtcgggcg ccgtccgtcg
     5761 ggcacgaact gcacatgcac ggtgccgaag ctgccgggct tgagcggcgg gccgacgacc
     5821 tcggcgtgcg cacccgtgat gaacaggcgg ccgtcctgca ggcgctcgaa gccatcgccc
     5881 ttcacgcctt ccttcaccca ttcgagcagg gcctcgccgc ggaactcgac catcaggtgg
     5941 ccccagtcga acagcgagaa gccgcgccgc ttcggcgtgc ggaacagcag ccgcgtggcg
     6001 cgccgttcgc gcgtgaagcg tccgatcacc acgtccgcgt agcgcgcccc gtcgccgtcg
     6061 gtgtcgacga tggagatgtt cttccacacg atgttgttgt tgttctgcac gttggcgtag
     6121 aggttggcag tctcggcgaa gctcatgccg aagggcgcgg tggcggaggt ctcgatgcgg
     6181 gcgagcaggc agaagtgcgc cttgtccgcg ccgaaggccg catagtccga aggatccggc
     6241 ggcgtccacg cgaactccac gatctcggtg tcgccgccgt tcacgctcac cgcctggctg
     6301 ccgacgagcc cgcccatcag cgccgggctg gccacgctgc cgtcccaggg cgccggccac
     6361 gacagcgagc tcgacgcctt cgcccaatac agcttgagcg tgccgctctg cgccgcggcg
     6421 ccggcacagc cgcggttgcg cacgcgcaca tagacgtagt tcgtctgctc gccgttgggg
     6481 ttctggtggt cctggttggt gaggccgtcg gcgacgttgc gcacccagat gtcgtcgctg
     6541 atccacatcg ggttggtgct cgcatccggc tcggcgccga cgtcgtcggc gttgtcctgc
     6601 atccacaggt cgggcgcggg cgagccgccg c
//
LOCUS       DAOQNN010000160         6608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6472_length_6608_cov_8.344422, whole genome shotgun sequence.
ACCESSION   DAOQNN010000160 DAOQNN010000000
VERSION     DAOQNN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6472_length_6608_cov_8.344422"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKV97_09645"
     CDS             complement(<1..469)
                     /locus_tag="PKV97_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNB06178.1"
                     /translation="MSKQGMGNFAESEAAARGLAYDDIWLVAGRRTPFADYNTVLRDV
                     SPTDLGIAAARALFAASGIPAAEVDAIVAGNMAQSSFDAYYLPRHIGLYSGVNPNVPA
                     LLVQRLCCTGFETILTAADHITLGKANVVLCVGAESMSRNPVAAYTHRAGFRMG"
     gene            complement(466..1236)
                     /locus_tag="PKV97_09650"
     CDS             complement(466..1236)
                     /locus_tag="PKV97_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNB06179.1"
                     /translation="MTEQLVKTTSEDGIYTLLLARPDKRNAISDRLLAALEAALVATP
                     EGTRAIILAGEGKHFCAGLDLSEHQHRDAFGTMLHSQSWHRIFARIQNGGIPVIAAMQ
                     GAVIGGGLELATAAHIRVAEPSTIYQLPEGRHGIYVGGGASVRVAKIVGAGRMCEMML
                     TGRILDADEGQRLGLSHYLVGAGETLAKAQELARRVAENAPMANWAMVTAIARIDNMA
                     SDDGFFVESLTAAVVQTNPEIAARIGEFLQRKGQGARQ"
     gene            complement(1260..3137)
                     /locus_tag="PKV97_09655"
     CDS             complement(1260..3137)
                     /locus_tag="PKV97_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HNB06180.1"
                     /translation="MAEIIRDAARLFADPDLIIRHRPNGDRILRSGIELPDAIPRCVG
                     DWLEHWAAVQPDEVFLAERNAAGEWDRLSYGEARRRVVAIASWLLGQKLSAERPVVIL
                     SDNGIEHALLALAAMHVGVPVAPISPGNSLMSKDFGKLRGNFELLRPGVVFADPIERF
                     LPAIAAVADLHDGVVVAGSRSQPQPGVLPIAQIEAQAAAKADEAAVMAAFAAVGPDTI
                     AKFLFTSGSVGTPKAVINTQRMMCSNQLAKELVWPFLRSNRPVVCEWLPWSHTFGGNH
                     DFNMVLRWGGSFYIDDGKPTPALIEKTLRNLREVSPTIYFSVPRAYDMLVPHLRQDAA
                     LRERFFRRLQVIMYAGAALPQHLWNELEALSEASVGARIPMVSSWGSTETAPLATDCH
                     FQAVRAGVIGVPVPGTELKLVPSHGKLEVRVRGPNVFTGYWKQPELTAKSFDDEGYYL
                     IGDAVEFVDAAHPERGLLYDGRVGEDFKLLTGTWVHVGSVRVAGIDAMSPIAQDIVVT
                     GHDRDEIGFLIFPNLPECRRLCPHLAPDADIIDLLLNPAIRSRVAQGMAAMIKKGGGS
                     STHPTRAILMAEPPSVEAGEITDKGYINQRAVLTRRADLVEFLYADVLDKTVITVHPA
                     A"
     gene            complement(3177..4316)
                     /locus_tag="PKV97_09660"
     CDS             complement(3177..4316)
                     /locus_tag="PKV97_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB06181.1"
                     /translation="MKLAKLVVALSATFTLSSAAFADITIGVSLSATGPAASLGIPEK
                     NAFALLPTEIGGEKVKLVVLDDATDPTVATKNARRLVTEDRVDAIIGSSSTPATAAMA
                     EVAHETKTPLVTMSPVSLPVERNEWVFRTPQQNEVMASALVEHMKSKGYKTLGFIGFS
                     DAYGEDWLNSVTKHAGAAGIQLTVVERYNRNDTSVTGQVLKLTTARPDAVLVAGSGSP
                     AALPQGALVERGYKGQIYQTHAAANQAFLGVAGKAAEGAILPVGPVSVVRQLPDSHPS
                     KASGVDFVTKYEAQYGAGSFSAFAGYAHDAFRLIEAAVPAAKAKGAPGTAEFRAALRD
                     ALEASKEVVGVHGVFNMTPNDHFGLDDRSRVLIRIEGGAYKLVAQ"
     gene            4604..5497
                     /locus_tag="PKV97_09665"
     CDS             4604..5497
                     /locus_tag="PKV97_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB06182.1"
                     /translation="MNAARPDGAAMAPMTDTEAAILQLAREAPELGQVAVAERLRSEG
                     LRISPSGVRYLWQKHGLETAVKRLHALEEGRGAEALTEAQRRLLERGLLTERLARGEQ
                     AEDGAGKEQEPTERRRLILDAAARLFSAEGYDRTSIRDIARNVGLLPGSVYHYFPSKR
                     ELFLAVHREGFEHTLERVREAAASGGDPWECLRRACEAHVEGIVGGTSVDRLAGSNLA
                     TAQNDELLNEIRPHRAAYETVFKTLIDALPLRAGTDRSLLRLFLLGGMNWVYLWYREG
                     KRSPREIADAMVDMLRSGVQP"
     gene            complement(5523..6056)
                     /locus_tag="PKV97_09670"
     CDS             complement(5523..6056)
                     /locus_tag="PKV97_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNB06183.1"
                     /translation="MSLAHALLTSLLEQPSSGYELARRFDKSIGHFWQATHQQIYREL
                     GRMEKAGWIRSDVDPDAGRTRKRSYSVLPTGRDELMRWAAEASPLPELRDEFMVRLRA
                     DAVTGPLGLLPELDRRRALHADKLRAYRAIEARDFPAEVEPTRQRRIHHLILKAGIQY
                     EQGWIAWYDEAIGVLGE"
     gene            6279..>6608
                     /locus_tag="PKV97_09675"
     CDS             6279..>6608
                     /locus_tag="PKV97_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dienoyl-CoA reductase"
                     /protein_id="HNB06184.1"
                     /translation="MATYPNLFRPLDLGFTTLPNRFLMGSMHVGLEEAEGGFERMAAF
                     YAERVRGGVGLIVTGGIAPNLEGRPWSGGATLTTSEEAARHRVITDAVHREGGKIALQ
                     ILHFGRYA"
BASE COUNT          988 a         2227 c         2371 g         1022 t
ORIGIN      
        1 gccccatgcg gaagcccgcg cggtgggtgt aggccgccac cgggttgcgc gacatcgact
       61 cggcgccgac gcagagcacg acgttcgcct tgccgagcgt gatgtggtcg gcggcggtga
      121 ggatggtctc gaagccggtg cagcacaggc gctgcaccag cagggccggc acgttgggat
      181 tgacgcccga atacagcccg atgtggcgag gcaggtagta ggcgtcgaag ctcgactgcg
      241 ccatgttgcc ggcgacgatc gcgtcgacct cggcggcggg gatcccgctg gcggcgaaca
      301 gcgcgcgcgc cgcggcgatg ccgaggtcgg tcggcgagac gtcgcgcagc acggtgttgt
      361 agtcggcgaa cggcgtgcgg cggccggcca ccagccagat gtcgtcgtag gcgaggccgc
      421 gcgcggccgc ctccgactcg gcgaagttac ccatgccctg cttgctcatt gacgtgctcc
      481 ctggcccttg cgctggagga attcgccgat gcgtgcggcg atctcggggt tggtctggac
      541 gaccgcggcg gtcagcgact cgacgaagaa gccgtcgtcg ctggccatgt tgtcgatgcg
      601 ggcgatcgcg gtgaccatcg cccagttggc catcggcgcg ttctcggcga cgcggcgtgc
      661 gagctcctgc gccttggcga gcgtctcgcc cgcgcccacc aggtagtgcg acaggccgag
      721 gcgctggccc tcgtcggcgt cgaggatgcg gccggtgagc atcatttcgc acatgcgccc
      781 ggcgcccacg atcttcgcca cgcgcaccga ggcgcccccg ccgacgtaga tgccgtggcg
      841 gccttcgggc agctggtaga tcgtgctggg ctcggcgacg cggatgtgcg ccgcggtggc
      901 cagctctagc ccaccgccga tcaccgcgcc ctgcatcgcg gcgatcaccg ggatgccgcc
      961 gttctggatg cgggcgaaga tgcggtgcca ggactgcgag tgcagcatgg tgccgaaggc
     1021 gtcgcggtgc tggtgctcgg agaggtccaa ccccgcgcag aagtgcttgc cctcgcccgc
     1081 caggatgatg gcgcgggtgc cctcgggggt ggcgaccagc gccgcctcga gcgccgcgag
     1141 caggcggtcg ctgatcgcgt tgcgcttgtc cggacgggcc agcaggaggg tgtagatgcc
     1201 gtcctcggac gtggtcttga cgagctgttc ggtcattgct tgttcccttt cgatgaaaat
     1261 cacgccgccg gatggaccgt gatgacggtc ttgtcgagca cgtcggcgta gaggaactcg
     1321 accaggtctg cgcggcgggt cagcaccgcg cgctggttga tgtatccctt gtcggtgatc
     1381 tccccggcct cgaccgaggg cggctccgcc atcaggatgg cgcgcgtggg atgggtggag
     1441 gagcccccgc ccttcttgat catcgccgcc atgccctgcg cgacgcggct gcggatcgcc
     1501 gggttgagca gcaggtcgat gatgtcggcg tcgggtgcca gatgcgggca caggcggcgg
     1561 cattcgggca ggttggggaa gatcaggaag ccgatctcgt cgcggtcgtg gccggtgacg
     1621 acgatgtcct gcgcgatcgg cgacatggcg tcgatccccg ccacccgcac cgagcccaca
     1681 tgcacccagg tgccggtgag gagcttgaag tcctcgccga cgcggccgtc gtagagcagg
     1741 ccgcgctcgg ggtgcgccgc atcgacgaac tcgacggcgt cgccgatcag gtagtacccc
     1801 tcgtcgtcga aggacttcgc ggtcagctcg ggctgcttcc agtagccggt gaagacgttg
     1861 ggccccctga cccgcacctc gagcttgccg tgcgagggca cgagcttgag ctcggtgccg
     1921 ggcaccggca ccccgatcac gcccgcgcgc accgcctgga agtggcagtc cgtggccagc
     1981 ggcgcggtct cggtggagcc ccacgaggac accatgggga tgcgcgcgcc gacgctcgcc
     2041 tcggacaacg cctcgagctc gttccagagg tgctgcggca gtgccgcacc ggcgtacatg
     2101 atcacctgca ggcggcggaa gaagcgctcg cgcagggcgg cgtcctggcg caggtggggc
     2161 accagcatgt cgtaggcgcg cggcacgctg aaatagatcg tcggcgacac ctcgcgcagg
     2221 ttgcgcaggg tcttctcgat cagcgcgggc gtgggcttgc cgtcgtcgat gtagaagctg
     2281 ccgccccagc gcagcaccat gttgaagtcg tggttgccgc cgaaggtgtg gctccacggc
     2341 agccactcgc acaccaccgg gcggttgctg cgcaggaagg gccacaccag ctccttggcg
     2401 agctggttcg agcacatcat gcgctgggtg ttgatgaccg ccttcggcgt gcccaccgaa
     2461 cccgaggtga acaggaactt ggcgatggtg tcggggccga ccgcagcgaa ggccgccatg
     2521 acggccgcct cgtcggcctt cgcagcggcc tgcgcctcga tctgcgcgat gggcagcacg
     2581 ccgggctgcg gctggctgcg actgccggcg acgacgacac cgtcgtgcag gtcggccacc
     2641 gccgcgatcg cgggcaggaa gcgctcgatc gggtcggcga agaccacgcc cgggcgcagc
     2701 agctcgaagt tgccgcgcag cttgccgaag tccttcgaca tcagcgagtt gcccggcgag
     2761 atcggcgcca ccggcacgcc gacgtgcatc gccgccagcg ccagcagggc atgctcgatg
     2821 ccgttgtccg agaggatcac caccggccgc tcggccgaga gcttctggcc gagcagccag
     2881 gaggcgatcg ccacgacgcg gcggcgggcc tcgccatagc tgagccgatc ccattcgccg
     2941 gcggcgttgc gctcggcgag gaagacctcg tcgggctgca ccgcggccca gtgctccagc
     3001 cagtcgccca cgcagcgcgg gatcgcatcc ggcagctcga tgcccgaacg caggatgcgg
     3061 tcgccgttgg gccggtgccg gatgatcagg tccgggtcgg cgaacaggcg tgcagcgtcg
     3121 cgaatgatct cagccatggc ctcgtctcct ccttttagcg tcgcggcggc tcgcacttac
     3181 tgcgcgacca gcttgtaggc gccgccttcg atgcggatca gcacgcggga acggtcgtcc
     3241 aggccgaagt ggtcgttcgg ggtcatgttg aacaccccat gcacgcccac cacctccttg
     3301 ctcgcctcga gcgcatcgcg cagcgcggcg cggaactcgg cggtaccggg cgcgcccttg
     3361 gcctttgccg cgggcactgc cgcctcgatc aggcggaagg cgtcgtgggc atagccggca
     3421 aaggcggaga agctgcccgc gccgtactgg gcctcgtact tcgtgacgaa atcgacaccg
     3481 gaggccttgg agggatggct gtcgggaagc tgcctgacga ccgagaccgg ccccaccggg
     3541 aggatcgcgc cttcggccgc cttgccggcg acaccgagga aggcctggtt ggcggcggcg
     3601 tgggtctggt agatctggcc cttgtagccg cgctcgacca gcgcgccctg cggcagcgcg
     3661 gcggggctgc ccgaaccggc gaccagcacg gcatccgggc gcgccgtggt gagcttgagc
     3721 acctggccgg tcaccgaggt gtcgttgcgg ttgtagcgct cgaccacggt gagctggatg
     3781 ccggccgccc cggcgtgctt ggtcaccgag ttcagccagt cctcgccgta ggcgtcggag
     3841 aagccgatga agccgagcgt cttgtagccc ttggacttca tgtgctcgac cagcgcgctc
     3901 gccatcacct cgttctgctg cggcgtgcgg aagacccact cgttgcgctc gaccggcagg
     3961 gacaccgggc tcatcgtcac cagcggggtc ttggtctcgt gcgccacctc ggccatcgcg
     4021 gcggtggccg gggtgctgga cgaaccgatg atcgcatcca cgcggtcctc ggtgaccagg
     4081 cggcgggcat tcttggtggc cacggtggga tcggtggcgt cgtccagcac gaccagcttg
     4141 accttctcgc cgccgatctc ggtcggcagc agggcgaagg cgttcttctc ggggatgccg
     4201 agcgaggcgg ccgggccggt ggccgagagc gagaccccga tggtgatgtc ggcgaacgca
     4261 gcggagctga gggtgaaggt ggcggagagc gcgacgacga gcttggcgag tttcatgttt
     4321 cctccaattg gctggtgtgg tgaccgctac ggcaagggcc ggcggaaagt cggctgctct
     4381 ctcgcgggac tgcagctctt ctaggctgtg cgcaagatat cgcttcacta actaccttgt
     4441 caaacacttg ttcaattact ttttcgattg accttcgggc atcatctacc ggaacatcca
     4501 gccgtccccc agggccacga agccgcgcgt tcagctcgct tttcgctgcg atgcggcgga
     4561 aaatccgctc cggatgagag acacgacccg aaacgaaacc cccatgaacg cagcacgacc
     4621 cgacggcgcc gcaatggcgc ccatgaccga cacggaggcc gcgatcctcc agctcgcgcg
     4681 cgaggcgccc gagctcggcc aggtcgcggt ggcggaacgg ctccgcagcg aaggcctgcg
     4741 gatctcgccc tcgggcgtgc gctacctctg gcagaagcac ggcctcgaga ctgcggtgaa
     4801 gcgcctgcac gcgctggagg aaggcagggg cgcggaggca ctgacggagg cgcagcgccg
     4861 cctgctcgag cgcggcctgc tcaccgagcg cctggcgcgc ggcgagcagg cggaggacgg
     4921 agcgggcaaa gagcaggagc cgaccgagcg ccgccggctg atcctggatg cagctgcacg
     4981 cctgttctcc gcagagggct acgaccgcac ctcgatccgc gacatcgcac gcaacgtcgg
     5041 cctgctgccc ggctcggtct atcactactt cccgtccaag cgcgagctct tcctcgccgt
     5101 tcatcgcgag ggcttcgagc acacgctcga gcgcgtccgc gaagcggccg cgagcggcgg
     5161 cgatccctgg gaatgcctgc ggcgcgcctg cgaagcccac gtcgaaggca tcgttggggg
     5221 cacctcggtg gatcgcctgg cggggagcaa cctggcgacc gcgcagaacg acgagctgct
     5281 caacgagatc cgcccccatc gtgccgccta cgagaccgtg ttcaagacac tgatcgacgc
     5341 cctgcccctg cgcgcgggaa ccgatcgctc gctgctgcgc ctgttcctgc tgggcgggat
     5401 gaactgggtc tatctctggt accgagaggg caagcgctcg ccgcgcgaga tcgccgacgc
     5461 gatggtcgac atgctgcgca gcggcgtgca gccctgagcg acgcgcgtcc gccgcgcggc
     5521 gctcattccc ccagcacgcc gatggcttcg tcgtaccagg cgatccagcc ctgctcgtac
     5581 tggatgccgg ccttcaggat gaggtggtgg atgcgccgct ggcgggtggg ctcgacctcg
     5641 gcgggaaagt cgcgcgcctc gatggcgcga taggcgcgca gcttgtcggc gtggagcgcg
     5701 cgacggcgat cgagttcggg cagcaggccg agcggcccgg tgaccgcgtc ggcgcgcagg
     5761 cggaccatga actcgtcgcg caactcgggc aagggcgagg cctcggcggc ccagcgcatc
     5821 agctcgtcgc gcccggtggg cagcacgctg tagctgcgct tgcgggtgcg gccggcgtcg
     5881 gggtcgacgt cggagcggat ccaccccgcc ttctccatgc ggccgagctc gcggtagatc
     5941 tgctgatgcg tggcctgcca gaagtggccg atggacttgt cgaaacgccg tgcgagctcg
     6001 tagccggacg agggttgttc gagcagcgag gtgagcaggg catgggcgag cgacatgcac
     6061 gaagtctagc cgcgcgcagc aggagcatgc aacgcgttgc atagttggcg cgaatcttct
     6121 acagtcctgc aactggttgc atagtcggac ggccggtggg cggcgcaggc ctgcccctgc
     6181 cccgcgcgtc tctccctccc ctctcccgcg cgcggggcag cccctcccct gccgcccggc
     6241 catgcgacca gccccgataa aaatccgcga gcgagacgat ggccacctat ccgaacctgt
     6301 tccgtcccct ggacctcggc ttcaccaccc tgcccaaccg cttcctgatg ggctcgatgc
     6361 atgtcggcct ggaagaggcc gagggtggct tcgagcgcat ggccgcgttc tacgccgagc
     6421 gcgtgcgcgg cggggtgggg ctgatcgtga ccggcggcat cgcccccaac ctcgagggcc
     6481 ggccgtggag cggcggcgcc acgctgacca catcggaaga agccgcccgc caccgcgtga
     6541 tcaccgacgc ggtgcatcgc gagggcggca agatcgcgct gcagatcctg cacttcggcc
     6601 gctacgcc
//
LOCUS       DAOQNN010000161         6604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6478_length_6604_cov_9.326920, whole genome shotgun sequence.
ACCESSION   DAOQNN010000161 DAOQNN010000000
VERSION     DAOQNN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6478_length_6604_cov_9.326920"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1352)
                     /locus_tag="PKV97_09680"
     CDS             complement(84..1352)
                     /locus_tag="PKV97_09680"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HNB06185.1"
                     /translation="MHVMVLGAGLSGVTTAWYLTRAGFQVSLVDRQPGAGLETSFANG
                     GQISISHPEPWANPGAPAQVLRWLGRADAPLKFRPHRALAQWTWGLRFLLECLPARAH
                     RNTVAIAALALASGQRLRALRAELGLEYDQLERGILHLFYSRGEFAKAPARAAQLRAF
                     GIAAEVLDRDACLEREPALATCAGSLAGGIFAQGDESGDAHKFTRALAHRLEEGGVVL
                     HRETEVSALETGSGGRVVGARVRRADGSVDSLRADACVLCLGSHGPRLATPLGLRLPI
                     YPVKGYSITAPVLEPALAPYVSLTDESRRIVCSRLGDRLRVAGTAELDGFDTTIRAAR
                     IAPIMAWVQARFPHAIDLERAEPWAGLRPATPGNVPLIGRSARPGLWLNTGHGTLGWT
                     LACGSAAALADLMSGRRPEVAFPFLAAAEV"
     gene            1506..2507
                     /locus_tag="PKV97_09685"
     CDS             1506..2507
                     /locus_tag="PKV97_09685"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNB06186.1"
                     /translation="MRRVTLTQFLIEQQRAGRITADLRLLIEVVARAVKAISVNVSKG
                     ALAGVLGEAGTDNVQGEAQKKLDVIANEILLQANEWGGHLAGMASEEVETICQIPFDL
                     PKGGFLLLFDPLDGSSNIDVNISVGTIFSVLRNPPGGTEPTEESFLQPGREQVAAGYA
                     VYGPATQLVLTVGQGVHGFTLDREMGSFIYTQPFMTIPVDTQEFAINASNARFWEAPV
                     KRYVAEMVQGKEGPLGKDYNMRWVASMVADVHRILTRGGVFMYPMDEKCRSKGGKLRL
                     MYEANPMAMLVEQAGGAASTGRERILDVMPDRLHQRVPVILGSKNEVERVAGYHQED"
     gene            2512..4269
                     /locus_tag="PKV97_09690"
     CDS             2512..4269
                     /locus_tag="PKV97_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HNB06187.1"
                     /translation="MRSGVRHPLRRGLRFLALALAFAGGGALAEEPLEVRLQAPDEVR
                     PLLERHVRLLRQEALELPEEQADRSAMTRRARREVAGLLATEGYFSPRVRFDRSDARN
                     WVLEVEPGPRTEIAQVELQFVGEIATSGPGGSEYLDLLRAGWSLPVGAPFSQGGWDGA
                     KQALLDAVAARRYAAARIVDSRAEIDPESARATLRVRIDSGPSFYLGELDVQGLEHLP
                     TDLVERYSRLKPGAPYDREELLAFQTGLQNTPHFGSVIVDIERDPTLSAAVPVRVKVT
                     EALPRYAGVGAGYSTNTGARVEFSYRDANLRKRGWEFSSGLRIEERRQALFADVFLPP
                     RGRDRDSFGALYEASDLEGLKIDSQALGAARTTLRGDIETQLALRLQHENIEPAGAPS
                     RSTNTLSANWTWKKRAVDNLLDPTSGYVLEFQVGGGSKTLFSDQDFLRFYSRVARYQP
                     VRGTDVFILRGEAGVTLADSREGVPQDFLFRTGGAQSVRGYDYQSLGVKEGDATVGGR
                     YLATASAEYVRWFRPQWGAAVFVDAGDAADTRADYDLRVGYGVGARWRSPAGPLAIDL
                     AWGHQERSLRLHFGVAVAF"
     gene            4445..6046
                     /gene="groL"
                     /locus_tag="PKV97_09695"
     CDS             4445..6046
                     /gene="groL"
                     /locus_tag="PKV97_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322108.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNB06188.1"
                     /translation="MTARQLLFRDDARHRILRGVSLLADAVKETLGPRARTVLLQQPY
                     GPPIVINSGVVVARAIELADPFENMGAQLVREVAARTSEVAGDGTTTATLLAAAIVRE
                     GMKHVAAGMNPMDLKRGVDKAVDALVGALKAMARPCGTRTEIAQVASISANNDAAIGE
                     LIADAMERVGKDGVITVEEGSGLASTLDVVEGMQFDRGYLSPYFINTEGARVVLEDAV
                     ILVCDRRIGAVAELLPVLEVVARSGRPLLIIAEEVEGEALALLVVNAARGTLKACAVK
                     APEFGERRHAMLEDIAVLAGATLVGGETGISLEHATAAELGSARRIEVDKDRCTLVGG
                     GGAHALIEARIARIRAELEGTRSDFEREGPEKRIARLAGGVAVVKVGGATESEMKERR
                     ARVEDALHATRAAVAEGILPGGGVALLRARAALGVLHGVNHDEDCGIQIVLRAVEEPL
                     CQLVANGGLEPSVVLDQVAAGSGAFGFNAATEEYGDLIAMGVLDPCKVTRSALQNAAS
                     VAGLILTTDCLVADLPQAGAVAPGG"
     gene            6043..6171
                     /locus_tag="PKV97_09700"
     CDS             6043..6171
                     /locus_tag="PKV97_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06189.1"
                     /translation="MSGRNEALRRCIHRKGDVETAAMGLLMRGPGFGSVAEAALLA"
     gene            6247..>6604
                     /locus_tag="PKV97_09705"
     CDS             6247..>6604
                     /locus_tag="PKV97_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNB06190.1"
                     /translation="MQRPAIIHKTAADLAVPPNLADYAATCAGFSWDAARAELAGLPG
                     GGLNIAHEAVERHAHGPLRERIAFRFLGESSLRDVGYGELSRLTSRFANVLRTLGVRR
                     GERVFVLTGRIPELYIA"
BASE COUNT          936 a         2286 c         2439 g          943 t
ORIGIN      
        1 ggcgaggccg cgcgcgcggc ctgatgcggc ctcctccgcg atgcgttcgg cccgccgcaa
       61 cgcgtcgccg ggcgcacgcg gcgctagacc tccgccgccg cgaggaaagg aaacgccacc
      121 tcgggcctgc gcccgctcat cagatccgcc agtgcggcgg ccgaaccgca ggccagcgtc
      181 catcccagcg tgccatggcc ggtgttgagc catagcccgg ggcgcgcgct gcgtccgatc
      241 agtggcacgt ttcccggcgt ggcggggcgc aacccggccc agggttccgc gcgttcgaga
      301 tcgatcgcgt gcgggaagcg cgcctgcacc cacgccatga tcggcgcgat ccgcgccgcg
      361 cggatcgtgg tgtcgaagcc gtcgagttcc gccgtgccgg cgacgcgcag gcggtcgccc
      421 aggcgcgagc acacgatccg gcgcgactcg tcggtcaggc tcacgtaggg tgcgagcgcg
      481 ggctcgagta ccggtgcggt gatcgagtag cccttgaccg ggtagatcgg cagccgcaag
      541 ccgagcggtg tggcgaggcg cgggccgtgg ctgcccaggc agagcacgca ggcgtccgcg
      601 cgcaggctgt cgacgctgcc gtccgcgcgg cgcacgcgcg cgcccacgac ccggccgccg
      661 gatccggtct cgagcgcgct cacctcggtc tcccggtgca ggaccacacc accttcttcc
      721 agcctgtgcg cgagcgcgcg ggtgaacttg tgcgcgtcgc ccgattcgtc gccctgggcg
      781 aagatcccgc cggccaggct gccggcgcac gtggccagcg cgggctcgcg ttccaggcag
      841 gcatcgcggt ccagcacctc ggccgcgata ccgaaggcgc gcagctgcgc agcgcgcgcg
      901 ggggccttgg cgaactcccc gcggctgtag aacaggtgca ggatgccgcg ctcgagctgg
      961 tcgtattcga ggccgagttc ggcgcgcagc gcacgcagtc gctgcccgct ggcgagcgcg
     1021 agcgcggcga tcgcgacggt gttgcggtgc gcccgtgcgg gcaggcattc gagcaggaaa
     1081 cgcaggcccc aggtccactg cgcaagggcg cggtgcgggc ggaacttcag cggcgcgtcc
     1141 gcgcgtccga gccagcgcag cacctgcgcg ggtgcgccgg ggttggccca gggctcggga
     1201 tggctgatcg agatctgccc gccgttggcg aagctggtct ccagtccggc gccgggctgg
     1261 cggtcgacca ggctgacctg gaagccggcg cgggtcaggt accaggcggt cgtgacgccg
     1321 gacaggccgg cgccgagaac catgacgtgc atgaagtgga aacgcgggtt gggggtgccg
     1381 atgatagcgc ctcggcggct gcggcagcac tctcgcggtt tcgggcatca cggcccgtgc
     1441 gctcgtggcg cggaaccgcg ataatcccgc cttgtccgtt tcgttcaatg tcgtggagcc
     1501 ttgtcatgcg ccgagtcacc cttacccagt tcctcatcga gcagcagcgc gccggccgca
     1561 tcaccgccga cctgcgcctg ctgatcgagg tcgtcgctcg cgcggtcaag gcgatcagcg
     1621 tcaacgtctc caagggcgcg ctcgccggcg tgctcggcga ggccggcacc gacaacgtcc
     1681 agggcgaggc gcagaagaag ctcgacgtca tcgccaacga gatcctgctg caggccaacg
     1741 agtggggcgg ccatctcgcc ggcatggcct cggaggaagt cgagaccatc tgccagatcc
     1801 ccttcgacct gcccaagggc ggcttcctgc tgctcttcga tccgctcgac ggctcgtcca
     1861 acatcgacgt caacatctcg gtcggcacca tcttctcggt gctgcgcaac ccgccgggcg
     1921 gcacggagcc caccgaggag agcttcctgc agcccggtcg cgagcaggtc gcggccggct
     1981 acgccgtgta tggtcctgcc acccagctcg tgctcaccgt ggggcagggc gtgcacggat
     2041 tcacgctcga ccgcgagatg ggcagcttca tctacaccca gcccttcatg accatcccgg
     2101 tcgataccca ggaatttgcc atcaacgcat ccaatgcgcg cttctgggag gcgccggtca
     2161 agcgctacgt cgccgagatg gtgcagggca aggaaggtcc gctcggcaag gactacaaca
     2221 tgcgctgggt ggcctcgatg gtcgcagacg tgcatcgcat cctcacccgc ggcggcgtgt
     2281 tcatgtaccc gatggacgag aagtgtcgca gcaagggcgg caagctgcgc ctgatgtacg
     2341 aggccaaccc gatggcgatg ctggtcgagc aggctggcgg cgcggcctcc accgggcgcg
     2401 agcgcatcct cgacgtgatg ccggaccggc tgcaccagcg cgtgccggtg atcctcggct
     2461 cgaagaacga ggtcgagcgc gtcgcgggct accaccagga ggactgagcg ggtgcgctcg
     2521 ggagtgcgac acccgctgcg ccgggggctg cgcttcctcg cgctggccct cgcgttcgcg
     2581 gggggtggcg cgctggcgga ggagcccctg gaggtgcgcc tgcaggcgcc cgacgaggtg
     2641 cgaccgctgc tcgagcgcca cgtgcgcctg ctgcgccagg aggcgctcga actgcccgag
     2701 gagcaggccg accgctcggc gatgacccgg cgggcgcgtc gcgaggtggc gggcctgctc
     2761 gccaccgagg gctacttctc gccgcgggtg cgcttcgatc gcagcgacgc gcgcaattgg
     2821 gtgctggagg tggaacccgg tccgcgcacc gagatcgcgc aggtcgagct gcagttcgtc
     2881 ggcgagatcg ccacctccgg gccgggcggc agcgagtacc tcgacctgct gcgcgcgggc
     2941 tggagcctgc cggtcggcgc gcccttcagc cagggcggct gggacggcgc caagcaggcg
     3001 ctgctcgatg cggtcgcggc gcgccgttac gccgcggcgc gcatcgtgga cagccgcgcc
     3061 gagatcgacc cggagagcgc gcgtgccacg ctgcgggtgc gcatcgacag cggaccgagc
     3121 ttctaccttg gcgagctcga cgtgcagggc ctggagcatc tgccgaccga tctcgtcgag
     3181 cgctacagcc ggctcaagcc cggtgcgccc tacgaccgcg aggagctgct tgcctttcag
     3241 accggactgc agaacacccc gcacttcggt tcggtgatcg ttgacatcga gcgcgacccg
     3301 acgctgtccg cagcggtgcc ggtgcgggtc aaggtcaccg aagcactgcc gcgctatgcc
     3361 ggggtggggg cgggctattc gaccaacacc ggcgcgcgcg tcgagttcag ctatcgcgac
     3421 gccaacctgc gcaagcgcgg ctgggagttc tcctccggcc tgcgcatcga ggagcgccgc
     3481 caggcgctgt tcgccgacgt cttcctgccg ccgcgcgggc gcgaccgcga cagcttcggc
     3541 gcgctctacg aggccagcga cctcgaaggg ctcaagatcg actcgcaggc gctcggtgcc
     3601 gcgcgcacca cgctgcgcgg cgacatcgag acccagctcg cgctgcgcct gcagcacgag
     3661 aacatcgaac cggccggcgc gccctcgcgc tcgaccaaca ccctgagcgc gaactggacc
     3721 tggaagaagc gcgcggtcga caacctgctc gaccccacca gcggctacgt gctcgagttc
     3781 caggtcggcg gcggcagcaa gacgctattc tccgaccagg acttcctgcg tttctactcg
     3841 cgcgtcgcgc gctaccagcc ggtgcgcggc accgacgtgt tcatcctgcg cggcgaggct
     3901 ggcgtgaccc tggccgacag ccgcgagggc gtgccgcagg atttcctctt ccgcaccggc
     3961 ggcgcgcagt cggtgcgcgg ctacgactat cagagcctgg gcgtgaagga gggcgacgcg
     4021 accgtcggcg gccgctacct ggcgaccgcg agcgccgagt acgtgcgctg gttccggccg
     4081 cagtggggcg cggccgtctt cgtcgatgcg ggggatgccg ccgatacccg cgcggactac
     4141 gatcttcgcg ttggctacgg cgtcggcgcg cgctggcgca gcccggcggg cccgctcgcg
     4201 atcgatctcg cctggggcca ccaggaacgc agcctgcggc tgcatttcgg ggtcgcggtg
     4261 gcgttctgag gcgcggcgct cgatgggctg ccatcggccg atcggcgtct gcgcgcgcag
     4321 cgctgccctt cgcccccgcg ccgtccgcgt tctgctcgct tccctggtcg cctcgccatt
     4381 gcccgcggaa caggagtaga ttttcacggt gatccgcgcg gtgaggccga acagggagga
     4441 aacgatgacc gccagacaac tgcttttccg cgacgacgcg cgccaccgca tcctgcgcgg
     4501 cgtgtccctg ctggccgatg cggtgaagga gacgctgggg ccgcgcgcgc gcaccgtgct
     4561 gctgcagcag ccctatgggc cgccgatcgt gatcaactcc ggggtcgtgg tcgcgcgcgc
     4621 gatcgagctc gccgatccct tcgagaacat gggcgcccaa ctggtgcggg aggtggcggc
     4681 gcgcacttcc gaagtggcgg gcgacggcac caccaccgcg accctgctgg ctgcggccat
     4741 cgtgcgcgag ggcatgaagc acgtcgccgc gggcatgaac ccgatggatc tgaagcgcgg
     4801 cgtcgacaag gctgtcgacg cgctcgtcgg cgcgctgaag gcgatggcgc ggccgtgcgg
     4861 gacgcgcacc gagatcgccc aggtggcgag catctcggcc aacaacgacg ctgccatcgg
     4921 cgagctgatc gccgacgcca tggagcgcgt cggcaaggac ggcgtgatca ccgtggagga
     4981 gggctcgggc ctcgcgagca cgctggacgt ggtcgagggc atgcagttcg accgtggtta
     5041 cctgtcgccc tatttcatca acaccgaggg tgcgcgcgtc gtgctcgagg atgccgtcat
     5101 cctcgtgtgc gaccgcagga tcggcgccgt ggccgagctg ctgccggtgc tcgaggtcgt
     5161 ggcgcgcagc gggcggccct tgctgatcat cgccgaggaa gtcgagggcg aggcgctggc
     5221 gctgctggtg gtgaacgccg cgcgcggcac gctgaaggcc tgcgcggtga aggcgccgga
     5281 gttcggcgag cgccgccacg ccatgctcga ggacattgcg gtgctggccg gcgccacgct
     5341 ggtcggcggc gagaccggca tcagcctgga acacgccacc gcggccgagc tgggcagcgc
     5401 gcgccgcatc gaggtggaca aggaccgctg cacgctggtg ggtggcggcg gcgcgcacgc
     5461 gctgatcgag gcccgcatcg cccgcatccg cgccgagctc gagggcacgc gctccgactt
     5521 cgagcgcgag gggccggaga agcgcatcgc gcgcctggcc ggcggcgtgg cggtggtgaa
     5581 ggtgggtggg gccaccgagt ccgagatgaa ggagcgccgt gcgcgtgtcg aggacgcgct
     5641 gcatgcgaca cgcgccgcgg tggccgaagg catcctgccc ggcggcggcg tggcgctgtt
     5701 gcgcgcgcgc gcggcgctgg gcgtgctgca tggcgtcaac cacgacgagg actgcggcat
     5761 ccagatcgtg ctgcgcgcgg tggaggagcc gctgtgccag ctcgtcgcca acggcggcct
     5821 ggaaccttcc gtggtgctgg accaggtggc ggcgggcagt ggcgcgttcg gcttcaatgc
     5881 cgcgaccgag gaatacggcg acctcatcgc gatgggcgtg ctcgacccgt gcaaggtcac
     5941 gcgcagtgcg ctgcagaatg cggcctcggt ggcgggcctg atcctcacca ccgattgcct
     6001 ggtggccgac ctgccgcagg ccggggctgt tgcgccgggc gggtgagcgg ccgtaacgag
     6061 gcgctgcgcc gctgcattca tcgcaagggg gacgtcgaaa cggcggcgat gggcctcctc
     6121 atgcgcggcc cgggcttcgg cagcgtggcc gaggcggccc tgctggccta aaaagaaacc
     6181 ggtgaggtgg ccgacgcgaa gccgtcgctg ccatgcgacg atgaagacga tggtgaggag
     6241 aaggcgatgc agcgccccgc gatcatccac aagacggccg ccgatctggc cgtgccgccc
     6301 aatcttgccg actacgccgc gacctgtgcc ggcttctcct gggacgcggc gcgcgccgag
     6361 ttggcagggc tgcccggcgg cgggctcaac atcgcccatg aggcggtcga gcgccacgcg
     6421 catggcccgc tgcgcgagcg catcgccttc cgtttcctcg gtgagagctc gctccgtgat
     6481 gtcggctacg gcgagctgtc gcgcctcacc agccgcttcg ccaacgtgct gcggaccctg
     6541 ggcgtgcggc gcggcgagcg tgtcttcgtg ctgaccgggc gcatcccgga gctgtacatc
     6601 gcgg
//
LOCUS       DAOQNN010000162         6532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6586_length_6532_cov_8.799753, whole genome shotgun sequence.
ACCESSION   DAOQNN010000162 DAOQNN010000000
VERSION     DAOQNN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6586_length_6532_cov_8.799753"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1117)
                     /locus_tag="PKV97_09710"
     CDS             complement(116..1117)
                     /locus_tag="PKV97_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNB06191.1"
                     /translation="MARTAASLATRLRHAGERWLFRIGAPEPAPIRLGQRRIYVLPSA
                     AGVGFALALLVMLLAAINYNLSLGYALVFTLGGSAAASILHAFRNLYGLSIRPGRCAP
                     VFAGETAVFSLLIDNPASRRRPALRLGAQGQWSAFDLPAAEETAVALACPALRRGRLA
                     LGRSVLETRWPLGLIRAWSVFVPKADCLVLPTPEADPPPLPPTGGDATGASRDARTGD
                     EDFAGLRAHRSADSPRHVAWKAVARGGPMLTKEFAAGEGRELLLDWDRLPAGLDAERR
                     LSRLTAWVLAAEREGRRYALALPGIRIAAATGRAHRERCLRLLALHGLPDPTAEDGA"
     gene            complement(1117..2034)
                     /locus_tag="PKV97_09715"
     CDS             complement(1117..2034)
                     /locus_tag="PKV97_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNB06192.1"
                     /translation="MTLHHANRLLEAASRIILGKERELRLALACLIARGHLLIEDVPG
                     VGKTTLAHALARLMGLQFQRIQFTSDLLPADILGVSIFDRNTAAFRFHPGPVFAQLIL
                     ADEINRATPKTQSALLEAMEERQVTADGQTCALPEPFFVIATQNPAHQIGTFPLPESQ
                     LDRFLLRIRLGYPDPQAERALLMGEDRRGLLERQPAVIGPGELLDMQLAAQGLHVAEA
                     LVAYVQTLLAATRNSAELAGGLSPRAGLGLLAVARAWALLDGRDHVLPEDVQTLFPHV
                     AAHRLHLAGDGRPATAATLVRLLQTVAVG"
     gene            2188..3114
                     /locus_tag="PKV97_09720"
     CDS             2188..3114
                     /locus_tag="PKV97_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNB06193.1"
                     /translation="MTTTAFITHRDCWLHDMGAMHPECPDRLSAINDRLIASGLDMYV
                     SFFDAPLAEREHILRVHPAEHLDNLIESVPEHGIRHLDPDTAMSPGTMKAALRSAGAG
                     VYATDLVMKGEIENAFCAVRPPGHHAERAKAMGFCFMNNVAIAARHALEAHGLERVAV
                     VDFDVHHGNGTEDIFRNDPRVMMAGIFQHPFYPYCGTENPPAHMCNVPLPAGTRGDAF
                     RQVFGDIVVPALRSHSPQMIFISAGFDAHYEDDMGSLGLLEADYIWATQQIKGVAESC
                     GHKRIVSILEGGYSLSSLARSVVAHIKTLADL"
     gene            3257..4135
                     /gene="dapA"
                     /locus_tag="PKV97_09725"
     CDS             3257..4135
                     /gene="dapA"
                     /locus_tag="PKV97_09725"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNB06194.1"
                     /translation="MITGSLVAIVTPMHDDGSLDFPRLRSLIDWHIAEGTDGIVIVGT
                     TGESPTVTVDEHCELIRVAVEHCAGRVPVIAGTGANATAEAVELARFAAQAGANAHLS
                     VVPYYNRPTQEGLYRHFRTIAEAVELPLILYNVPGRTVADLANDTVLRLAEVPNIVGL
                     KDATGNLDRACDLVERAPAEFALYSGDDMTSAAFLMLGGHGVISVTANVAPRAMHALC
                     AAAAAGDMRTLRATNAGLTGLHRDLFCEANPIPVKWAVARMGLIGDGLRLPLTPLSAA
                     HHERVRAAMRKAGIQA"
     gene            4162..5304
                     /gene="bamC"
                     /locus_tag="PKV97_09730"
     CDS             4162..5304
                     /gene="bamC"
                     /locus_tag="PKV97_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNB06195.1"
                     /translation="MIRSKRTSLSLLALSIALGGCSGSLLESKRIDYKSAKQIERPLE
                     IPPDLTAPQRDDRFAVPDVAPRGVATYSAYTADRAGQPAQATSGPLVLAKSDTMRIER
                     AGSQRWLVVGGTPEELWPQIKDFWLELGFILNIDSPEIGVMETDWAEDRAKIPQDFLR
                     SSIGKVLDGLFSTPERDKFRTRLEQGKETGTVEVYISHRGMMEIYPTEAKDSTIWQPR
                     PADPELEAEMLRRLMVRLGAEEARAEVAIATPQQAERARIASAPDGQVMLVMDEAFDR
                     AWRRVGLALDRIGFTVEDRDRAQGLYFVRYVDPDADNRSTEKGFLSRLAFWRSDAKPA
                     AGGSEYRLRVQGQGEASRVSVLTREGGLDNSDTARRMLGLLREQLR"
     gene            complement(5436..5861)
                     /locus_tag="PKV97_09735"
     CDS             complement(5436..5861)
                     /locus_tag="PKV97_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06196.1"
                     /translation="MKHSILAAALVALAVSACGKKEEPVAVQPAPSAQEQMKESAAQA
                     VEAAKESAKAAGDAAAAGAQAVKQEAAAAVEATKEAAATAAAKADAAADAAAKKADAA
                     VEAVKDAAAEAKADTVDAAAQAKAAAEAAVGAAKESAAK"
     gene            6105..>6532
                     /locus_tag="PKV97_09740"
     CDS             6105..>6532
                     /locus_tag="PKV97_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNB06197.1"
                     /translation="MPTAKIDSLDHEGRGVTRVEGKAVFVEGALPGERVEYVVRRAKP
                     SYEQGEVVRVLRPSAQRVAPRCPHYGVCGGCSMQHLDADAQAAVKQRLLEDALRHIGK
                     LHADRILPAIHGPAWGYRYRARLGVRLVPSKGGLRVGFHER"
BASE COUNT          980 a         2257 c         2320 g          975 t
ORIGIN      
        1 agcagccagg cgccctggtc gcgccgcagc gcaggcgccg ggggtccggt gcgtgtgcca
       61 cccagcgggc cgccgcggcg gcggaggccg atcagccgcc gtgcgcgagc cacgctcatg
      121 cgccatcctc cgctgtcgga tcgggaaggc catggagcgc gagcaggcgc aggcagcgct
      181 cgcgatgggc gcggccagtt gctgccgcga tgcggatgcc gggcagggcg agcgcgtagc
      241 ggcgtccttc acgctcggcg gcgagtaccc aggcggtgag ccgcgacagt cggcgctcgg
      301 cgtcgagtcc ggcgggcagg cggtcccagt cgagcagcag ctcgcgcccc tctcctgcgg
      361 cgaactcctt ggtcagcatc gggccgccgc gcgccacggc cttccatgcg acgtggcggg
      421 gcgagtccgc gctacggtgc gcgcgcaagc cggcgaagtc ctcgtcaccc gtgcgcgcgt
      481 cgcggctggc gccggtcgcg tcgccacctg tggggggcag gggcggcgga tcggcttccg
      541 gcgtgggcag caccaggcag tcggccttgg ggacgaacac gctccacgcc cggatgaggc
      601 cgagcggcca gcgcgtctcg agcacgctgc gcccgagcgc gagccggccg cgccggagcg
      661 cggggcaggc cagggcgacg gcggtctctt ccgcggcggg gagatcgaac gcgctccatt
      721 gtccctgcgc gcccaggcgt aaggcggggc gccgtcggct ggccgggttg tcgatgagga
      781 gggagaacac ggcggtctcg cccgcgaaca ccggcgcgca gcgccccggg cggatcgaca
      841 gaccgtacag gttgcggaag gcgtgcagta tgctcgccgc ggcgctgccg ccgagggtga
      901 acacgagcgc gtagccgagg ctcaggttgt agttgatcgc cgcgagcagc atcaccagca
      961 gggcgagggc gaagccgaca cctgccgcgc tcggcagcac gtagatgcgg cgctggccga
     1021 ggcggatcgg agcaggctcc ggggcgccga tgcggaacag ccagcgctcg ccggcgtggc
     1081 gcagccgcgt ggcgagcgat gcggcggtgc gcgccatcag cccacggcga cggtctgcag
     1141 cagccgcacc agcgtcgccg ccgtcgccgg gcggccgtcg ccggcgaggt gcaggcggtg
     1201 cgcggcgacg tgcgggaaga gggtctgcac gtcctccggc agcacgtggt cgcggccgtc
     1261 gagcagtgcc catgcacgcg ccacggcgag cagcccgaga ccggcacgcg gactcagtcc
     1321 gccggcgagt tccgcactgt tgcgggttgc ggcgagcagg gtctggacgt aggcgacgag
     1381 ggcctcggcg acatgcaggc cttgcgccgc cagctgcatg tcgagcagtt cgcccgggcc
     1441 gatgacggcc ggctggcgct cgagcagccc gcgccggtcc tcgcccatca gcagcgcgcg
     1501 ctcggcctgc gggtccgggt agccgaggcg gatgcgcagc aggaagcggt ccagctggct
     1561 ctcgggcagc ggaaaggtgc cgatctggtg ggcggggttc tgcgtggcaa tcacgaagaa
     1621 gggttcgggc aacgcgcagg tctggccgtc ggcggtgacc tggcgctcct ccatggcttc
     1681 cagcagcgcg ctctgcgttt tcggcgtggc gcggttgatc tcgtcggcga ggatgagttg
     1741 ggcgaacacc ggcccggggt ggaagcggaa ggcggcggtg ttgcggtcga agatggagac
     1801 cccgaggatg tccgcgggca gcaagtcgct ggtgaactgg atgcgctgga actgcagccc
     1861 catcaggcgg gcgagcgcgt gtgcgagcgt ggtcttgccg acgccgggca cgtcctcgat
     1921 cagcaggtgg ccgcgcgcga tcaggcaggc gagcgcgagg cgcagctcgc gctccttgcc
     1981 gaggatgatg cgcgaggcgg cttcgagcag acgattcgca tggtgcaggg tcatcgtgcg
     2041 cactgtgaaa ttggcctgga agcggtgata atagacgaca aaggcaagcg gcccgacgat
     2101 cggacgcaac acggtcggcg gccgccagag tcggaagggg gtgtctggtg ccccgtgtcc
     2161 gtcgtccgcg aggcgagagg gaggagcatg accacgacag cattcattac tcaccgtgac
     2221 tgctggctgc acgacatggg tgcgatgcat cccgagtgtc ccgaccggct gtcggcgatc
     2281 aacgatcgcc tgatcgcctc ggggctcgat atgtatgtgt cctttttcga tgcgccgttg
     2341 gccgagcgcg agcacatcct gcgcgtgcat ccggccgagc atctcgacaa cctgatcgag
     2401 agcgtgccgg agcacggcat ccgccacctc gatcccgaca ccgcgatgag tccgggcacg
     2461 atgaaggcgg cgctgcgttc cgccggtgcg ggggtctacg ccaccgacct cgtgatgaag
     2521 ggggagatcg agaacgcctt ctgtgccgtg cgtccgccgg gccaccacgc cgaacgcgcc
     2581 aaggcgatgg ggttctgctt catgaacaac gtcgccatcg ccgcccgcca tgcgctcgaa
     2641 gcccacgggc tggagcgcgt cgcggtggtc gacttcgacg tgcaccacgg caacggcacc
     2701 gaagacatct tccgcaacga cccgcgggtc atgatggcgg ggatcttcca acatcccttc
     2761 tacccgtatt gcggtaccga gaaccccccg gcgcacatgt gcaacgtgcc gctgccggcg
     2821 ggtacgcggg gcgatgcctt ccgccaggtc ttcggcgaca tcgtcgtgcc cgcgctgcgc
     2881 agccatagtc cgcagatgat cttcatctcc gccggattcg acgcgcacta cgaggacgac
     2941 atgggctcgc tcgggctgct cgaggccgac tacatctggg cgacccagca gatcaaggga
     3001 gtcgccgagt cctgcggcca caagcgcatc gtctcgatac tcgaaggcgg ctattcgctg
     3061 tcctcgctgg cgcgctcggt agtggcccac atcaagacgc tggccgatct ctgagtcccg
     3121 ctcgccgggc cgcctgcgtt gccgccggcc ggcgcgcccg catacggggc acgccggccg
     3181 cgtcctgtaa cagaggcagg ctcgaccgtg cgcggcgatc ggttagaatc ccagctttaa
     3241 ttcactgtcg gacgccatga ttaccggatc gcttgtcgcc atcgtcacgc ccatgcacga
     3301 cgacggcagc ctggacttcc cccgcctgcg cagcctcatc gactggcaca tcgccgaagg
     3361 taccgacggc atcgtgatcg tcggcacgac cggcgagtcg cccacggtca ccgtggacga
     3421 acattgcgag ctgatccgcg tggccgtcga gcactgcgcg gggcgcgtgc cggtgatcgc
     3481 cggcaccggt gcaaacgcca ccgccgaagc ggtggaactc gcgcgcttcg ccgcacaggc
     3541 cggcgcgaac gcgcatcttt cggtggtgcc ttactacaac cggcccaccc aggaaggcct
     3601 ctaccgccac ttccgtacca tcgccgaggc ggtcgaactg ccgctgatcc tctacaacgt
     3661 tcccggccgt accgtcgccg acctcgccaa cgacaccgtg ctgcgccttg ccgaggtgcc
     3721 caacatcgtc ggtctcaagg acgccaccgg caacctcgac cgcgcctgcg accttgtcga
     3781 gcgcgcgccg gcggagttcg cgctgtacag cggcgacgac atgacttcgg ccgcctttct
     3841 gatgctcggc ggccatggcg tcatctcggt gaccgccaac gtcgcgccgc gcgcgatgca
     3901 tgcgctgtgc gcggccgcgg ccgcgggcga catgcgcacc ttgcgcgcaa ccaatgccgg
     3961 gctcaccggc ctgcatcgcg acctgttctg cgaggccaac ccgatcccgg tgaagtgggc
     4021 ggtggcgcgc atggggctga tcggcgacgg cctccgcctg cccctcaccc cgctgtccgc
     4081 agcccatcac gagcgcgtgc gcgcggcgat gcgcaaggcc ggcatccagg cctgatccga
     4141 atctctctgc ccaggacttc catgatccgc tccaagcgca cctccctgtc cctgctcgcg
     4201 ctctcgatcg cgctcggcgg ttgttcggga tcgctgctcg aatccaagcg catcgactac
     4261 aagagcgcga aacagatcga acgccccctc gagatcccgc ccgatctcac cgcaccgcag
     4321 cgcgacgacc gcttcgccgt gcccgatgtc gccccgcgcg gcgtcgccac ctactccgcc
     4381 tacacggccg atcgcgccgg ccagccggcg caggccacca gcggtcccct ggtgctcgcc
     4441 aaatccgaca ccatgcgcat cgagcgtgcg ggcagccagc gctggctggt ggtgggcggc
     4501 acccccgagg aactgtggcc gcagatcaag gatttctggc tcgagctcgg cttcatcctc
     4561 aacatcgaca gcccggaaat cggcgtcatg gagaccgact gggccgagga tcgcgccaag
     4621 atcccgcagg atttcctccg ctccagcatc ggcaaggtgc tcgacggcct gttctcgacc
     4681 cccgagcgcg acaagttccg cacccgcctc gaacagggca aggaaacggg cacggtggag
     4741 gtctacatct cgcaccgcgg catgatggaa atctacccca ccgaggccaa ggactcgacg
     4801 atctggcagc cgcgtcccgc cgaccccgag ctcgaggccg agatgctgcg tcgcctgatg
     4861 gtccgcctcg gtgccgagga ggcgcgtgcc gaggtcgcga tcgccacgcc gcagcaggcg
     4921 gagcgggcgc gcatcgcctc cgcgcccgac ggccaggtga tgctggtgat ggacgaggca
     4981 ttcgatcgtg cctggcgtcg cgtcggcctc gcgctcgacc gcatcggctt caccgtcgag
     5041 gaccgcgacc gtgcgcaggg cttgtacttc gtgcggtacg tcgatcccga cgcggacaac
     5101 cgcagcaccg agaaaggctt cctgtcgcgg ctggccttct ggcgcagcga cgccaaaccg
     5161 gccgccggtg gcagcgaata ccgcctgcgc gtgcaggggc agggcgaggc ctcgcgggtc
     5221 agcgtgctca cccgcgaggg cgggctcgac aactccgaca ccgcgcgccg catgctgggc
     5281 ctgctgcgcg aacaactgcg ctgaactaca agggggagag ggcctcaacg aggccgtatc
     5341 ccccgaccgc gggagcggtc ttctgcgaag cgacagcatg aaaaaagccg gcatgcagcc
     5401 ggcttttttc attcgagccg tggagcgggg gcttcttact tcgcagccga ttccttggcg
     5461 gcgccgacgg cagcttcggc ggccgccttg gcttgggccg cggcatcgac ggtgtcggct
     5521 ttggcttcgg cggcggcgtc cttgaccgcc tcgaccgcgg cgtcggcctt cttcgcagcc
     5581 gcgtcggcag cagcgtccgc cttggcggcc gcagtagcgg cggcttcctt ggtcgcctcg
     5641 accgccgcgg cggcctcttg cttgacggcc tgcgcgccgg ccgcggcagc gtcgccggcg
     5701 gccttcgcgc tctccttggc cgcttccacc gcctgggcgg ccgattcctt catctgttcc
     5761 tgggccgagg gggccggctg aaccgcgacc ggctcttcct tcttgccgca ggcagacacg
     5821 gcgagggcga cgagggcggc ggcgaggatg gaatgcttca tggtctgtct tccttgttct
     5881 gggggtcggg gtgcgtacgg cttgtttcgg cggacgccgg gagaccccgt cttccgcgct
     5941 ggatgccgca atgcacaaag attctagcat gggggatcat gtcgtccgga tgaggtggcg
     6001 gcgcgcaagt ggggcccaaa ggtaaggaga cattgcctga agcggacatc cgcgcaggcg
     6061 tggcgcggta gaatcgcgcg cttcccgaac cctctcgcac gaccatgccg acagccaaga
     6121 tcgattccct ggaccacgaa ggccgtggcg tgacccgcgt cgagggcaag gcggtattcg
     6181 tcgagggcgc cctgccgggc gagcgcgtcg aatacgtcgt gcggcgtgcc aaaccgagtt
     6241 acgagcaggg tgaggtggtg cgcgtgctgc gcccgagcgc gcagcgcgtg gcgccgcgct
     6301 gcccgcatta cggcgtgtgt ggtggctgct ccatgcaaca tttggatgcg gatgcgcagg
     6361 ccgcggtgaa gcagcgcctc ctcgaggacg cgctgcgcca catcggcaag ctgcacgccg
     6421 accgcatcct gccggcgatc cacggtcccg cctggggcta ccgctaccgc gcgcgcctgg
     6481 gcgtgcgcct ggtgccgagc aagggtgggc tgcgcgtggg ttttcacgag cg
//
LOCUS       DAOQNN010000163         6503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6635_length_6503_cov_8.474256, whole genome shotgun sequence.
ACCESSION   DAOQNN010000163 DAOQNN010000000
VERSION     DAOQNN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6635_length_6503_cov_8.474256"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="PKV97_09745"
     CDS             complement(<1..132)
                     /locus_tag="PKV97_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transporter MerT family protein"
                     /protein_id="HNB06198.1"
                     /translation="MNARHTLWASALSAVGASLCCVAPLVLVTLGIGGAWVASLTAFE
                     "
     gene            181..594
                     /gene="merR"
                     /locus_tag="PKV97_09750"
     CDS             181..594
                     /gene="merR"
                     /locus_tag="PKV97_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HNB06199.1"
                     /translation="MYGVNMDKLTISALAREAGVNVETIRFYQRKELLPEPSRPPGGI
                     RHYGAGEVARVRFIKAAQRIGFTLEEIAQLLRLEDGTHCREAREIAEHKLADVRHRIA
                     DLRRIETALAALIDRCVAGRGQVTCPLIASLQEVA"
     gene            591..929
                     /locus_tag="PKV97_09755"
     CDS             591..929
                     /locus_tag="PKV97_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HNB06200.1"
                     /translation="MMELLRISVLFAVTAVAEIVGCYLPWLVLKQDKPAWLLIPAAAA
                     LALFAWLLTLHPFAAGRTYAAYGGMYIAVALAWLYWVDRVVPTRWDLAGAAVAVVGMA
                     IIVLQPALDG"
     gene            1016..1279
                     /locus_tag="PKV97_09760"
     CDS             1016..1279
                     /locus_tag="PKV97_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06201.1"
                     /translation="MIRTSASFDFGSSGGGLFDEAGNLVGLLGLKARSGKNLRFALPV
                     ESVQADALAFRVVDADSKGDALWARPKQSQPEFLGVAVQEAAR"
     gene            complement(1304..1642)
                     /locus_tag="PKV97_09765"
     CDS             complement(1304..1642)
                     /locus_tag="PKV97_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNB06202.1"
                     /translation="MKEIKAFVRQHRIADVLQALRDSGLCEPGSSSGCHNITVAQVLR
                     PLAGADPAQQHYSMELAEAVVAEYKLELVCGDDAVDALSDVIARAAHTGQAEGGWVFV
                     SEIQRAVEIP"
     gene            complement(1656..4763)
                     /locus_tag="PKV97_09770"
     CDS             complement(1656..4763)
                     /locus_tag="PKV97_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNB06203.1"
                     /translation="MLNHIVDLSLRYKLLVLLGFALVVFFGARAWLTVPVDAFPDVTP
                     NQVNVYTESPGLAAEDVEKLLTAPIETAMAGLPGVEQIRSVSLFGLSYVGVYFKDDID
                     IYFARRLVGEKLQEAKERLPEGYGEPSLGPNSSGLGQVFWYTVESADQKLSAMDLRTL
                     HDWSVRLMLRTAPGVDDVTTWGGEEKQYQVLIHPQKLIKYGLSFKAVMEALAANNRQV
                     GGQYVNLGQEQYLVRGLGLVANTTDIGNIVIAEREGVPIHVHEVAEVKEGPALRFGAV
                     TRDGKEVALGIALARINENAKNVVDAVKQKLELARQALPQGVTINPVYDRTDIVDKAL
                     KTAESALVEGSILVAIVLFLFLGEVRSALVVIVMLPLAMLIAFMLMQYFGLSANLMSL
                     AGLAIGIGMMVDGAVVMVENGFRLLSHQAGKAASRTQVPRTHVILEAAREVINPIAFA
                     ILIIIVVFLPLFSLTGLEGKLFKPMALTITFAMAGSLVLTLTLVPVLSALILKAKEEK
                     DTFLVRWVKAAYLPLLDRALENKKTVIAAALLLLVAALATFPFLGKEFMPTLQEGAIM
                     FRVTGIPSTSLEESVRVSQQMDAVLKKAFPQTRSVLATIGRAEKGETTDANYMEVLVD
                     VKPQDEWPEKLSMPVLSDRMKETLEQALPTVVLGNTQPIQMRVEELISGVRATLALKL
                     YGPDLATLDRLAGEIKPVLGAVPGVADLSLEANKGKPQLVIKVNREEAARFGINANEI
                     LELVQAGIGGKAVSTLIDGVRRFDIQVRLDGAFRDSPQAIADIPLRTQTGALVPLSRV
                     ATVETDEGYTFVRREQLSRYAVLQMDVKGRDVDGFVRDADAKLRSAVKLPEGYWIEWG
                     GAFENQQRAMARLALIVPLTIGLIFVLLYTAFNSLTHATLIIANVPFAIIGGVFGLAL
                     SGQYVSVPSAIGFIAVFGVAMLNGIVLVSFLNDQRRHGLSIRESVRQGASLRLRPVLM
                     TASVAILGLIPMLLSQGVGAETQRPLATVVVGGLITSTALTLLLLPLMYEWVETRRER
                     RNVVT"
     gene            complement(4776..6029)
                     /locus_tag="PKV97_09775"
     CDS             complement(4776..6029)
                     /locus_tag="PKV97_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB06204.1"
                     /translation="MKNTYPSRRAELLALGVAALILAGCGKSEQPQTSSYQVEAKQAP
                     AGKPASHEEGKEDGHEHGEADRLTLGAEEIRAAGIKVESLAEQEISEQVTVTATIRPN
                     QDRIAHVAPRVPGRIVRADVNLGDAVKAGQTLAVLDSLEVGEAHSSYLQAQTAHALAR
                     ADFERAEKLQSEQIIAQKDYLRARAESEKAKAALAAAADRLRMLGVNPAPAANAKAVS
                     TFPLTTPFAGTVIEKHAILGELAQTDKQLFTVADLTTLWIEANLFEKDLGRVKVGAQA
                     AVTVAAYPGEIFRGRLTYIAAVVDKETHTVQARVEVGNADGRLKPEMFATAAIATAGT
                     GGEAKLRALVVPEDAVVLMQGQPTVFVEEHGRFEPRPVELGDKLHGRVVVRNGLAAGD
                     KVVIAGTYALKARVMKSQLGEGHAH"
     gene            complement(6074..>6503)
                     /locus_tag="PKV97_09780"
     CDS             complement(6074..>6503)
                     /locus_tag="PKV97_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB06205.1"
                     /translation="FAGREGPGEARERITGLSVSLPLPLFRRNAAGIGKATTELDQTR
                     IERQAVLRDTRATVFALWQRLESLRARVQRLEQLVLQRLQENQRLSAAAYRAGEIDLT
                     RFLLATRQVLDTRREVLEATTELALTRVELEQAAGWTGTR"
BASE COUNT         1025 a         2181 c         2173 g         1124 t
ORIGIN      
        1 ctcgaatgcg gtgagcgagg ccacccacgc cccgccgatt ccgagggtga cgagcaccag
       61 cggcgccacg caacagagtg atgcaccgac cgccgagagc gcgctggccc acaaggtgtg
      121 tcttgcgttc atggcccttc caattcattc gtcagcacct gtacgataga ctccgtacct
      181 atgtacggag tcaacatgga taagctcacg atctccgcgc tcgcacgcga ggcgggcgta
      241 aatgtcgaaa cgatccgctt ctaccaacgc aaggagctac tcccggagcc gtccaggccg
      301 cccgggggca tccgccacta cggggccgga gaggtggcgc gagtgcgctt catcaaggcc
      361 gcccagcgca taggcttcac tcttgaggag atcgcgcaac tattgcgctt ggaggatggc
      421 acgcattgcc gggaagcccg cgagattgcc gaacacaagc ttgccgacgt ccggcatcgc
      481 attgccgacc tgcggcgcat cgaaaccgcc ctggccgcgc tcatcgaccg ttgcgtagcc
      541 ggtcgcggcc aggtgacctg cccgttgatc gcgtccctgc aggaggttgc atgatggaac
      601 tgctcaggat ttccgtcctg ttcgcggtca ccgcggtggc cgaaatcgtc ggctgctacc
      661 tgccgtggct cgtactcaag caggacaagc cggcatggct gctgattccg gcagcggcag
      721 cgctggcgct cttcgcgtgg ctgctcaccc tgcacccatt cgccgcgggg cgcacctatg
      781 ccgcctatgg cggcatgtac attgctgtgg ccctggcgtg gctctactgg gtggaccgtg
      841 tcgtccccac ccgctgggac ttggcggggg ccgcggtggc ggtcgtgggc atggcgatca
      901 tcgtgctgca gccggccctt gacggctgag agatgcgctc acgactcccg gatgtgcctg
      961 acgtgtgctc gcctcaggca gcgtcgaagg tctctatgcg caccacgcgg gcaaggtgat
     1021 ccgcacttcc gcgtccttcg acttcggctc gagcggcggc ggcctgttcg acgaggccgg
     1081 caacctcgtc ggcctgctcg gcttgaaggc gcgcagcggc aagaacctgc gcttcgcgct
     1141 gcccgtcgag tcggtccagg cggacgcgct cgccttccgc gtggtcgacg ccgactcgaa
     1201 aggcgacgcg ctctgggcac ggccgaaaca aagccagccg gaattccttg gcgtggctgt
     1261 gcaggaggcg gcccgctgag ctggcgccat cgcggctggg cccctacggg atctctaccg
     1321 cgcgctgaat ctcgctgacg aaaacccagc cgccttccgc ttgcccggta tgcgcggcgc
     1381 gggcgatcac atcgctcaac gcatcgaccg cgtcgtcgcc gcagacgagc tccagcttgt
     1441 attcggcgac gaccgcttcc gccagttcca tcgaatagtg ctgctgcgcc ggatcggcgc
     1501 ccgcgagcgg ccgcagcacc tgggcgaccg tgatgttgtg gcagccgctg ctgctgcctg
     1561 gctcgcacag gccggaatcg cgcagcgcct ggagcacatc ggcgatgcgg tgttgacgga
     1621 cgaatgcctt gatctctttc acgtgaatgt ctccttcagg tcacgacgtt cctacgctcc
     1681 cgccgcgtct ccacccactc gtacatgagc ggcaacagca gcagcgtgag cgccgtggag
     1741 gtgatcagtc cgccgaccac gaccgtggcc agcggccgct gggtttctgc gcccacgccc
     1801 tgcgagagca gcatcggaat caggccgagg atcgcgaccg aggcggtcat cagcaccggg
     1861 cgcaggcgca ggctcgcgcc ctggcgcacg gactcgcgga tcgacaggcc gtgacggcgt
     1921 tggtcgttga ggaaggacac cagcacgatg ccgttcaaca tcgcgacgcc gaacacggcg
     1981 atgaagccga tcgccgaggg caccgacacg tactggccgc tgagcgccag gccgaatacg
     2041 ccgccgatga tcgcaaaggg cacgttggcg atgatcagcg tcgcgtgggt cagcgaattg
     2101 aacgccgtgt agagcagcac gaagatcagc ccgatggtca gcggcacgat cagcgcgagc
     2161 cgcgccatcg cgcgctgctg gttttcgaag gcgccgcccc actcgatcca gtatccttcg
     2221 ggcagcttca cggcgctgcg gagcttcgcg tccgcgtctc tgacgaagcc gtccacgtcg
     2281 cggcccttca cgtccatctg cagcacagcg tagcgcgaga gctgctcgcg gcgcacgaag
     2341 gtgtagccct cgtcggtctc gacggtcgcg acgcgcgaca gcggcaccag cgcgccggtc
     2401 tgcgtgcgca gcgggatgtc ggcgatggcc tgcgggctgt cgcggaaggc gccgtcgagg
     2461 cgcacctgga tgtcgaagcg gcgcacgcca tcgatcagcg tgctcacggc cttgccgccg
     2521 atgcccgcct gcacgagctc gaggatctcg ttggcattga tgccgaagcg cgcggcctcc
     2581 tcgcgattta ccttgatgac gagctgcggc ttgcccttgt tggcttccaa cgagaggtcg
     2641 gccactccgg gcacggcgcc cagcacgggc ttgatctcgc ccgccaggcg gtcgagcgtg
     2701 gcgagatcgg ggccgtagag cttgagggcg agggtggcgc gcacgccgga gatcagttcc
     2761 tcgacgcgca tctggatcgg ctgggtgttg ccgagcacta cggtgggcag cgcctgctca
     2821 agggtctcct tcatgcggtc tgagagcacc ggcatggaga gcttctccgg ccactcatcc
     2881 tggggcttca cgtccaccag tacttccatg tagttggcat cggtggtctc gcccttctcc
     2941 gcgcggccaa tcgtggccaa caccgagcgc gtctggggaa aggccttctt gagcaccgcg
     3001 tccatctgct gcgagacgcg caccgattcc tccagcgagg tcgacggaat gccggtgacg
     3061 cggaacatga tggcgccctc ctgcagcgtg ggcatgaact ccttgccgag gaaaggaaag
     3121 gtggcgagcg ccgcgacgag cagcagcagc gccgcagcaa tcaccgtttt cttgttctcc
     3181 agcgcccggt cgagcagcgg cagataggcg gccttgaccc agcgcacgag gaaggtgtcc
     3241 ttctcctcct tggccttgag gatcagcgcc gagagcaccg gcaccagcgt gagcgtgagg
     3301 accagcgagc ccgccatggc aaaggtgatg gtgagcgcca tcggcttgaa cagcttgcct
     3361 tccaggccgg tgagcgagaa cagcggcagg aacacgacga tgatgatcag gatcgcgaag
     3421 gcgatcggat tgatcacctc gcgcgccgcc tcgaggatga cgtgggtgcg aggcacttgg
     3481 gtacgactcg ccgccttgcc ggcctggtgg gagagcaggc gaaaaccgtt ttccaccatc
     3541 accacggcgc cgtcgaccat catgccgatg ccgatggcga gccccgccag cgacatcagg
     3601 ttggccgaca ggccgaagta ttgcatcaac atgaaggcga tcagcatggc gagcggcagc
     3661 atgacgatca ccaccagcgc cgagcgcact tcgccgagga acaggaacag cacgatcgcg
     3721 accagaatcg agccttcgac cagtgcgctc tcggcggtct tcagcgcctt gtcgacaatg
     3781 tcggtgcggt catagactgg gttgatggtc acaccttgcg gcagcgcctg ccgcgcgagc
     3841 tcgagctttt gcttgaccgc gtctaccacg ttcttcgcgt tctcgttgat gcgcgcgagc
     3901 gcgatgccca gcgccacctc cttgccgtcg cgcgtcaccg cgccgaagcg tagcgccggg
     3961 ccttccttga cctcggccac ctcatgcacg tggatcggca cgccctcgcg ctcggcaatg
     4021 acgatgttgc cgatatccgt cgtgttggcg accaggccca ggccgcgcac caggtactgc
     4081 tcctggccga ggttcacgta ctgcccgccg acctggcggt tgttggcggc gagcgcctcc
     4141 atcaccgcct tgaacgaaag gccgtatttg atgagcttct gcgggtggat cagcacctga
     4201 tactgcttct cctcgccgcc ccaggtggtg acgtcatcga cccccggcgc cgtgcgcagc
     4261 atcaggcgca cgctccagtc gtgcagcgtg cgcaggtcca tcgcagagag cttctggtcg
     4321 gccgactcca cggtgtacca gaacacctgg ccgagacccg agctgttggg tccgagcgag
     4381 ggctcgccat agccttcggg caggcgctcc ttcgcttcct gcagcttctc gccgaccagg
     4441 cggcgcgcga agtagatgtc gatgtcatcc ttgaagtaga cgccgacgta ggacaggccg
     4501 aacagcgaca ccgagcggat ctgctcgacg ccgggcaggc cggccatggc ggtttcgatc
     4561 ggtgccgtca gcaacttctc gacgtcttcg gcggcgagtc cgggcgattc cgtatagacg
     4621 ttgacctggt tcggcgtgac atcgggaaag gcgtccaccg gcaccgtgag ccaggcacgc
     4681 gcgccgaaga agacgaccag cgcgaagccg agcagcacca gcagcttgta gcgcagcgag
     4741 agatcgacga tgtgattgag catggtgaac ccccctcagt gcgcgtggcc ttcgccgagc
     4801 tgcgatttca tgacgcgcgc cttgagggca taggtgccgg caatcaccac cttgtcgccc
     4861 gccgcgagtc cgttcctcac tacgacgcgg ccgtgcagct tgtcaccgag ctccacgggg
     4921 cgcggctcga agcggccatg ctcctcgacg aagaccgtgg gctggccctg catcagcacc
     4981 acggcatctt ctggcacgac cagcgcgcgc agcttcgcct caccgccggt gccggccgtt
     5041 gcgatggcgg cggtggcgaa catctcgggc ttgagtcgcc cgtcggcatt gccgacttcg
     5101 acgcgcgcct ggacggtgtg cgtctccttg tcgacgacgg cggcgatgta ggtaagcctg
     5161 ccgcggaata tctcgccggg ataggcggcg acggtgaccg cggcctgcgc gccgaccttg
     5221 acgcggccga ggtccttctc gaacaggttg gcctcgatcc acagcgtcgt caggtcggcc
     5281 acggtgaaga gctgcttgtc cgtctgcgcc agctcgccga ggatcgcgtg cttctcgatc
     5341 accgtgcccg cgaacggcgt ggtcagcggg aaggtggaga cggccttggc gttggcagcc
     5401 ggcgccgggt tcacgcccag catgcgcagc cggtcagcgg cggcggcgag cgcggccttg
     5461 gccttctcgg actcggcgcg ggcacgtagg taatccttct gcgcgatgat ctgctcgctc
     5521 tggagcttct cggcacgctc gaagtcggcc ctggccagcg cgtgcgcggt ctgcgcctgc
     5581 agataggacg agtgcgcttc gccgacttcg aggctgtcga gcacggcgag cgtctgcccg
     5641 gccttgaccg catcgccgag attcacgtcg gccctgacga tgcgtcccgg cacgcgcggc
     5701 gccacgtgcg cgatgcgatc ctggttggga cgtatggtcg cggtgaccgt cacctgttcg
     5761 ctgatctcct gctcggcgag gctctcgacc ttgatcccgg cggcgcggat ttcctccgcg
     5821 ccgagcgtga gccgatcagc ctcgccatgt tcgtgcccgt cttccttccc ctcttcatgc
     5881 gaagcgggtt tcccggccgg cgcctgcttc gcttcgacct ggtagctgga ggtctgcggc
     5941 tgctcgctct tgccgcagcc ggcgaggatc agcgccgcca cgccgagggc gagcaattcg
     6001 gcgcggcgcg atgggtaggt gtttttcatg aatgatcttt cgctgtctgg tttgttgttg
     6061 cgggcgcagg gcctcatcgc gtgcccgtcc agccggccgc ctgctcgagc tcgacgcgcg
     6121 tcagggcgag ttcggtcgtc gcctccagca cttcgcggcg cgtgtcgagc acctgccggg
     6181 tggcgagcag gaagcgggtg aggtcgatct cgccggcgcg gtaggcggcc gccgacaggc
     6241 gctggttctc ctgcaggcgc tgcagaacga gttgctccag ccgctgcacg cgggcgcgca
     6301 ggctttcgag ccgttgccac agtgcgaaga ccgtcgcgcg cgtgtcgcgc agcactgcct
     6361 ggcgctcgat ccgcgtctgg tccagctcgg tcgtcgcctt gccgatgccg gcggcattgc
     6421 ggcggaaaag cggcagcggc aacgagaccg agagcccggt gatgcgctcg cgcgcctcgc
     6481 ccggaccttc gcggccggcg aac
//
LOCUS       DAOQNN010000164         6474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6675_length_6474_cov_10.650413, whole genome shotgun sequence.
ACCESSION   DAOQNN010000164 DAOQNN010000000
VERSION     DAOQNN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6675_length_6474_cov_10.650413"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..721)
                     /gene="paaY"
                     /locus_tag="PKV97_09785"
     CDS             complement(104..721)
                     /gene="paaY"
                     /locus_tag="PKV97_09785"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HNB06206.1"
                     /translation="MNQLKVYAIDGIVPVVDPTAYVHPSAVLIGDVIVGPDCYVGPCA
                     SLRGDFGRLILERGSNLQDTCVMHGFPGTDTVVEEDGHIGHGAVLHGCRVGRNALVGM
                     NAVIMDNAVIGESSIVAAAAFVKAGVEMPPRMLIAGMPAKAIRALSEEEMRWKSSGTA
                     TYQDLTRRSLATMEETVPLAAVEPGRKRIHMPDVVPLVELRRREG"
     gene            805..1620
                     /locus_tag="PKV97_09790"
     CDS             805..1620
                     /locus_tag="PKV97_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNB06207.1"
                     /translation="MSDELDGKHGVQSLEVGMSILRAMVTGKRSMMLKDIAQAADMPP
                     SKAHRYLVSLIRSGLVEQDRLTSRYDLGPFALNLGLVALDRVDRIRLGLSAIGDLRDL
                     TNETVALAVWSDRGPVVVRWERPRRPITVNVLTGTSLSLLSSAAGRVFAAWLPEQQTE
                     ALLRAELESGRVPAGIATLDDARRLLAEVRARGVAVISSGYFARGVEAAAAPVFNFKN
                     EINMSIALVGVEGTLDLLPHSPVLQALKEACDALSRRLGATLLPGVESGDHAT"
     gene            1819..2967
                     /locus_tag="PKV97_09795"
     CDS             1819..2967
                     /locus_tag="PKV97_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB06208.1"
                     /translation="MKLRNTLLAALGLAFAATAAHAEIKVGVVLSATGPAASLGIPEK
                     NTIALLPATIGGEKVSYIVLDDASDTTTAVKNARKLTVEDGVDVIIGSTTSPASLAMV
                     DVAAETKTPMISMAASARIVAPMDDKKRWVFKTPQNDQQMASAIVEHMVANKVKKVSF
                     IGFANAYGEGWYEQFKKLAEAKGIEIAASERFNPADTSVTGQALKLMSVKPDAVFIAG
                     SGTPSALPQKTLRERGYKGPIYQTHGVANNDFLRICGKDCEGTLLPVGPVQMARSLPD
                     SHPVKASALAYVEKYEAANGAGSVSSFGAYAWDAGVLLQAAVPAALKAAKPGSAEFRT
                     ALRDALEGVKEVAGATGIYTMSPDDHLGLDDRSRVMIEIRNGTWSLLK"
     gene            3082..4119
                     /locus_tag="PKV97_09800"
     CDS             3082..4119
                     /locus_tag="PKV97_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB06209.1"
                     /translation="MDMSIALILSQDGITNGAIYALLALALVLVFAVTRVIFIQQGEF
                     VAYGALTLAMMQSGVLPATVWMLVAMGALVTVLDGGLALKAGQGRRAASVAGWNLGYP
                     LALAAVVGGLAVKELPLAVQVLLTLAIVVPMGPMMYRLMYQPIASAPVLVLLIVSVAL
                     HIAMVGLGLLFFGAEGQRTPAFSEFSLELGPLMVSAQTLWVVVVSVLLIVALYQFFER
                     TLYGKALRATAINRVGAQLMGISPTLAGKLTFFLAAFIGALSGVLIAPITTIYYDTGF
                     LIGLKGFVAAIIGGLASYPLAAVGALAVGLLEAFASFWASAYKEVIVFTLIIPVLLWR
                     SLRSHHVEEEE"
     gene            4116..5909
                     /locus_tag="PKV97_09805"
     CDS             4116..5909
                     /locus_tag="PKV97_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HNB06210.1"
                     /translation="MRTDRLVLGAFLALLLLVPAILSPYYVTLLNYIGLYAMVALGLV
                     LLTGVGGLTSFGQAAFVGLGAYTTAALTTATDLPGWLAWAGTSPWLTLAVGLLLTALV
                     AVLIGSLTLKLSGHFLPLGTIAWGISLYFLFGTMESLGGHTGISGIPAIGLLGWTLDE
                     GGEIYYLIWAFLLVAMLTTHNLLDSREGRAIRALKGGRVMAEAMGVDTSRSRMVIFVI
                     AALLACASGWLYAHMQRFVNPTPFGVHIGIEYLFMAVVGGAGYVWGAIVGAGVITVLK
                     QWLQNWLPSLLGATGNFETIVFGLLMVLVLHRAREGLWPILKKLVPVRVVRKAIDADA
                     EPLPRRTLPKAGELILEAKDVTRRFGGLIANNNMSLEVRAGEILALIGPNGAGKSTMF
                     NQISGVDTPTSGEVRFLGKPVAGHDSREIARMGMSRTFQHVKLLPTMSVLENVAIGAH
                     LRSDKGVLSSAWRLDRAEEARILKEAARQIERVGLAEYMYEEAGSLALGQQRILEIAR
                     ALCADPCLLLLDEPAAGLRFKEKEALGELLRKLRAEGMATLIVEHDMDFVMSLVDRVV
                     VMEFGQKIAEGLPDEIQQNPAVLEAYLGGVE"
     gene            5924..>6474
                     /locus_tag="PKV97_09810"
     CDS             5924..>6474
                     /locus_tag="PKV97_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB06211.1"
                     /translation="MNKQTQGSAAPSNADAKNPVLEVKDLCVSYGKVEALTDASLTVG
                     EGQIVTVIGPNGAGKTTMLSAIMGVLGSRGQVAFDGSVEGVPEVERMVARGMTLVPEK
                     RELFGEMSVEDNLVLGAFQRYRMGKRDHAQTLEEVYALFPRLKERRSQAAGTMSGGER
                     QMLAVGRALMAKPKLLMLDEPSLG"
BASE COUNT          990 a         2181 c         2226 g         1077 t
ORIGIN      
        1 cctgagcgcc gccagccttt tcccgccggt cggcgcgcgg cgaccggggc gggggcgtgc
       61 gggacgcgtt catggccgtg ccgccctgcg tgcgccgccg gcctcagcct tcccgcctgc
      121 gcagttccac cagcggcacc acgtccggca tgtggatgcg cttgcgcccg ggctcgaccg
      181 ccgcgagcgg cacggtctct tccatcgtgg ccaggctgcg gcgggtgagg tcctggtagg
      241 tcgcggttcc cgagctcttc cagcgcatct cctcctcgga gagcgcgcgg atcgccttgg
      301 ccggcatgcc ggcgatcaac atgcgcggcg gcatctcgac gccggccttg acgaaggccg
      361 ccgcggcgac gatcgatgac tcgccgatca ccgcgttgtc catgatcacg gcgttcatgc
      421 cgaccaaggc gttgcggccc accctgcagc cgtgcagcac ggcgccgtgg ccgatgtgtc
      481 cgtcctcctc gaccaccgta tcggtgccgg gaaagccatg catcacgcag gtgtcctgca
      541 ggttcgagcc gcgctccagg atcagtcgcc cgaagtcgcc gcgcaggctt gcgcaaggcc
      601 cgacgtagca gtccgggccc acgatcacgt cgccgatcag cacggccgac gggtgcacgt
      661 aggcggtcgg atccaccacc ggcacgatgc cgtcgatcgc atagactttc agctgattca
      721 tccttgttct cctccgctcg gacttatgtt acatataaag gaaagatttc ttcaataagt
      781 aattttatcg gtgagggggc ttcgatgagc gacgaactcg acggcaagca cggcgtgcaa
      841 tctctcgagg tgggcatgag catcctccgc gccatggtca ccggcaagcg ctccatgatg
      901 ctcaaggaca tcgcccaggc cgccgacatg ccgccctcga aggcgcaccg ctacctggtc
      961 agcctgatcc gcagcggact cgtcgaacag gaccggctca cctcgcgcta cgacctcggt
     1021 cccttcgccc tcaacctcgg cctggtcgcc ctcgaccgcg tcgaccggat ccgtctcggc
     1081 ttgagcgcga tcggcgattt gcgcgatctc accaacgaga ccgtcgcgct cgccgtctgg
     1141 agcgatcgag ggccggtcgt cgtgcgctgg gagcgtcccc gtcgtccgat caccgtcaat
     1201 gtgctgaccg gcaccagcct cagcctgctg agctcggccg ccgggcgcgt cttcgccgca
     1261 tggctgcccg agcagcagac cgaagcgctg ctgcgcgccg agctcgaatc cggtcgggtg
     1321 cccgcgggca ttgccaccct cgacgacgcg cgccggctgc tcgccgaggt ccgcgcgcgg
     1381 ggtgtggcgg tgatttccag cggctatttc gcccgcggcg tcgaggctgc ggcggccccg
     1441 gtgttcaact tcaagaacga gatcaacatg agcatcgccc tcgtgggcgt cgagggcacg
     1501 ctggatctcc tcccgcacag cccggtgctc caggcgctga aggaggcctg cgacgcgctg
     1561 tcgcggcgac tcggcgcgac cctcctcccg ggtgtcgagt cgggcgacca cgcgacctga
     1621 ggggtgggtg cggcgggagg ttgcgcacag caggagccgg atacttttgg aattgataca
     1681 aagtttctgc ttgacgggat cttttgtatc aattctaata actagaacgc gttctgcaat
     1741 gcagaacttg cgcgccccga gcgctgcgcc cccggcgcgc cggccccctc gcaccgcaca
     1801 accactggag gagacaccat gaagttgcgt aacaccctgc tggccgccct cggcctcgcc
     1861 ttcgccgcca ctgccgccca tgccgagatc aaggtcggtg tcgtgctctc ggccaccggc
     1921 cccgccgctt cgctgggtat cccggaaaag aacacgatcg cgctgctgcc ggccaccatc
     1981 ggcggcgaaa aggtgagcta catcgtgctc gacgacgcct ccgacaccac cacggcggtc
     2041 aagaacgccc gcaagctgac cgtcgaggac ggcgtcgatg tgatcatcgg ctccaccacc
     2101 agccccgctt cgctggccat ggtcgacgtc gccgccgaga cgaagacgcc gatgatctcg
     2161 atggccgcgt cggcgcgcat cgtcgcgccg atggacgaca agaagcgctg ggtcttcaag
     2221 accccccaga acgaccagca gatggcgtcg gcgatcgtcg agcacatggt cgccaacaag
     2281 gtgaagaagg tgtccttcat cggcttcgcc aacgcctacg gcgagggctg gtacgagcag
     2341 ttcaagaagc tggccgaagc caagggcatc gagatcgccg ccagcgagcg cttcaacccg
     2401 gccgacacct cggtgaccgg ccaggcgctc aagctgatgt cggtgaagcc ggacgcggtg
     2461 ttcatcgccg gctcgggcac gccctcggcg ctgccgcaga agacgctgcg cgagcgcggc
     2521 tacaaggggc cgatctacca gacccacggc gtggccaaca acgacttcct gcgcatctgc
     2581 ggcaaggact gcgaaggcac gctgctgccg gtcggcccgg tgcagatggc gcgcagcctg
     2641 cccgacagcc accccgtcaa ggcgagcgcg ctggcctacg tggagaagta cgaggccgcc
     2701 aacggcgcgg gctcggtgtc gagcttcggg gcctatgcgt gggatgccgg ggtgttgctg
     2761 caggcggccg tccctgccgc gctcaaggcg gccaagccgg gctcggcgga gttccgcacg
     2821 gcgctgcgcg atgcgctcga gggcgtgaag gaagtcgccg gcgccaccgg catctacacg
     2881 atgagccccg acgatcacct cggcctggac gaccgctcgc gcgtgatgat cgagatccgc
     2941 aacggcacct ggtcgctgct gaagtaagca tccccctccg cggcggggcg cctggcgtcc
     3001 cgccgcgcgt ctgtcgggga tttccgacac atcgaatcaa tcacggcggt ccgcgaggca
     3061 ccgccgagac gaggcatttc catggatatg tcaattgcat tgatcctgtc gcaggacggg
     3121 atcaccaacg gcgcgatcta cgcgctgctg gcgctggcgc tggtgctggt gttcgcggtc
     3181 acgcgggtga tcttcatcca gcagggcgag ttcgtggcct acggggcgct gacgctggcg
     3241 atgatgcagt cgggcgtgtt gccggccacg gtgtggatgc tggtggcgat gggagcgctg
     3301 gtcacggtgc tcgacggcgg gctcgcgctc aaggcggggc agggccggcg tgccgcgagc
     3361 gtggcgggct ggaacctggg ctatccgctg gcgctcgccg cggtggtcgg cgggctggcg
     3421 gtcaaggagc tgccgctggc ggtgcaggtg ctgctgacgc tggcgatcgt ggtgccgatg
     3481 gggccgatga tgtaccgcct gatgtaccag ccgatcgcat cggcgccggt gctggtgctc
     3541 ctgatcgtct cggtggcgct gcatatcgcg atggtgggcc tgggactgct gttcttcgga
     3601 gcggaaggcc agcgcacgcc ggcgttcagc gagttcagcc tcgagctcgg cccgctgatg
     3661 gtctccgcgc agacgctgtg ggtggtggtg gtgtcggtgc tgctgatcgt ggcgctgtac
     3721 cagttcttcg aacgcacgct ctacggcaag gccttgcgcg ccaccgcgat caaccgcgtc
     3781 ggtgcccagc tcatggggat ctcgcccacg ctcgccggca agctgacgtt cttcctcgcc
     3841 gccttcatcg gtgcgctctc gggcgtgctg atcgccccga tcaccacgat ctactacgac
     3901 accggcttcc tgatcggcct caaggggttc gtcgccgcca tcatcggcgg cctggcgagt
     3961 tatccgctgg ccgccgtggg tgcgctcgcc gtggggctgc tcgaagcctt cgcgtcgttc
     4021 tgggccagcg cctacaagga agtcatcgtg ttcaccctga tcattcccgt gctgctgtgg
     4081 cgctcgctgc gtagccacca cgtggaggaa gaagaatgag aaccgaccgt ctggtcctgg
     4141 gggcctttct cgccctgctg ctgctggtgc cggcgatcct gtcgccgtat tacgtgacgc
     4201 tgttgaacta catcggcctg tatgcgatgg tcgcgctcgg cctggtgctg ctcaccgggg
     4261 tgggcggact gaccagcttc ggccaggccg ccttcgtggg tctgggcgcc tacaccaccg
     4321 cggcgctcac caccgcgacc gacctgccgg gatggctcgc ctgggcgggg acttcgccct
     4381 ggctcaccct ggcggtcggg ctgctgctga ccgccctggt cgcggtgctg atcggctcgc
     4441 tcaccctgaa gctctcgggg cacttcctgc ccctgggcac gatcgcgtgg ggcatcagcc
     4501 tgtacttcct cttcggcacc atggagagcc tgggcggcca caccggcatc agcggcattc
     4561 cggccatcgg gctgctgggc tggacgctgg acgagggcgg cgagatctat tacctgatct
     4621 gggccttcct gctcgtcgcc atgctcacca cccacaacct gctcgactcg cgcgaggggc
     4681 gtgcgatccg cgccctcaag ggcggccgcg tgatggcaga ggcgatgggc gtggacacct
     4741 cgcgctcgcg catggtgatc ttcgtgatcg cggcgctgct ggcgtgcgcc tcgggctggc
     4801 tgtatgcgca catgcagcgc ttcgtgaacc cgaccccgtt cggcgtgcac atcggcatcg
     4861 agtacctctt catggccgtg gtcggtggcg ccggctatgt gtggggggcg attgtgggcg
     4921 cgggcgtcat caccgtgctc aagcagtggc tgcagaactg gctgcccagc ctgctcggcg
     4981 ccaccggcaa cttcgagacc atcgtgttcg gcctgctgat ggtgctggtg ttgcaccgcg
     5041 cccgcgaggg cctgtggccg atcctcaaga aactggtgcc ggtgcgcgta gtccgcaagg
     5101 cgatcgacgc cgacgccgag ccgctgccgc ggcgcacgct gcccaaggcg ggcgagctga
     5161 tcctcgaggc caaggacgtc acgcgccggt tcggcggcct gatcgccaac aacaacatga
     5221 gcctggaggt gcgcgcaggc gagatcctcg cgctcatcgg ccccaacggc gcgggcaaga
     5281 gcacgatgtt caaccagatc tcgggcgtcg atacgccgac ctcgggcgag gtgcgcttcc
     5341 tcggcaagcc ggtggccggc cacgactcgc gcgagatcgc ccgcatgggc atgagccgta
     5401 ccttccagca cgtgaagctg ctgccgacga tgagcgtgct cgagaacgtg gcgatcggcg
     5461 cccacctcag gagcgacaag ggcgtgttga gctcggcctg gcgcctggac cgcgcggaag
     5521 aggcccgcat cctcaaggaa gccgcccgtc agatcgagcg cgtgggcctg gccgagtaca
     5581 tgtacgagga ggccggcagc ctcgccctgg gccagcagcg catcctggag atcgcgcgtg
     5641 cgctgtgcgc cgacccctgc ctgctgctgc tcgacgagcc cgcggccggc ctgcgcttca
     5701 aggagaagga ggcgctcggc gagttgctcc ggaagctgcg cgcggagggc atggcgaccc
     5761 tgatcgtgga gcacgacatg gacttcgtga tgagcctggt cgatcgcgtg gtggtgatgg
     5821 agttcggcca gaagatcgcc gagggcctgc ccgacgagat ccagcagaac ccggcggtgc
     5881 tcgaggccta tctgggcggt gtggaatgag cggggagaac gcgatgaata agcagacgca
     5941 aggcagcgcc gcaccctcga acgcggacgc gaagaacccg gtgctcgagg tcaaggacct
     6001 gtgcgtgtcc tacggcaagg tcgaggcgct caccgatgcc agcctcaccg tcggcgaggg
     6061 ccagatcgtc accgtgatcg gccccaacgg agcgggcaag acgacgatgc tgtcggcgat
     6121 catgggcgtg ctcggctcgc gcggccaggt cgccttcgac ggcagcgtgg aaggcgtgcc
     6181 cgaggtcgag cgcatggtcg cgcgcggcat gaccctggtg cccgagaagc gcgagctctt
     6241 cggcgagatg agcgtcgagg acaacctggt gctgggcgcc ttccagcgct atcgcatggg
     6301 caagcgcgat cacgcgcaga cgctcgagga ggtctatgcg ctcttcccgc gcctcaagga
     6361 gcgccgcagc caggccgcag gcaccatgtc gggcggcgag cgccagatgc ttgcggtggg
     6421 ccgcgcgctg atggccaagc ccaagctgct gatgctcgac gagcccagcc tggg
//
LOCUS       DAOQNN010000165         6461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6712_length_6461_cov_8.063378, whole genome shotgun sequence.
ACCESSION   DAOQNN010000165 DAOQNN010000000
VERSION     DAOQNN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6712_length_6461_cov_8.063378"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..588)
                     /gene="rfbC"
                     /locus_tag="PKV97_09815"
     CDS             complement(52..588)
                     /gene="rfbC"
                     /locus_tag="PKV97_09815"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNB06212.1"
                     /translation="MKVIETALPGVLIIEPKAFGDHRGFFLETFQVERYREAGITLPF
                     VQDNHSRSQRGVLRGLHFQKTRPQGKLVSVSRGAVYDVAVDIDPSSATYGKFVGVELN
                     DDNHRQMWVPPGYAHGFCVLSEVADFQYKCTDFYFPADEGGLAWNDPDVGIPWPITEP
                     QLSAKDVNNPRLRDLKRA"
     gene            complement(585..1463)
                     /gene="rfbA"
                     /locus_tag="PKV97_09820"
     CDS             complement(585..1463)
                     /gene="rfbA"
                     /locus_tag="PKV97_09820"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNB06213.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILVISTPDDLPNFRKLLGDGSDFGIALSYAEQPSPDGLAQAFLIGEDFIGDDHV
                     CLILGDNIFYGYGFSAMLREAARRERGATVFGYHVSDPERFGVVEFDASGRAISIEEK
                     PKAPKSNYAVTGLYFYDNSVVEIARQVKPSARGELEITDVNNAYLQRGDLHVSLLGRG
                     FAWLDTGTHDSLMEASHFVQTIEARQGLKVACLEEIAYNQGWLSAAQLARQAEALSKT
                     GYGQYLKRLLRQEGGQ"
     gene            complement(1795..3378)
                     /locus_tag="PKV97_09825"
     CDS             complement(1795..3378)
                     /locus_tag="PKV97_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HNB06214.1"
                     /translation="MNTRLHHLAEAHNARFGTSGVRGLVSDLSDAVCYAYAQAFLRSI
                     ATDSARVVVGHDLRPSSPRIAAACIQAIRDAGREAVFVGALPTPALAYYCALQRAPGI
                     VVTGSHIPFDRNGIKFYRADGEIDKADEQAILDAEVAVPDALSPAPLPAVDASALEAY
                     VARYTAFFGEGALAGMRVALYEHSSVARDVLRTVFERLGAEVLSLGRTDTFVPIDTEA
                     VRAEDIAQARAWAQAHRFDAILSTDGDADRPLIGDETGTWLRGDVVGILCAQYLHADV
                     VVTPVSSNTAVEKCGAFRQVLRTRIGSPYVIAGMDTQRGAGRVVGYEANGGFLLGSDV
                     EYGEAIGASAAETAGVGAGSVGTGGFGSAEPGSTGFGSAEVATDGPEATQKCLPAGAA
                     RLKALPTRDSLLPMLALLAMARAKGCPVSQLADALPRRFTASDRIQNVPTEFSRALIA
                     RLQQTPAEAAALMAPDAGDVIAIDTTDGLRTSFATGEIVHLRPSGNAPELRCYAEAAD
                     EPRARALCDACLARIAASA"
     assembly_gap    3455..3554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3758..4321)
                     /locus_tag="PKV97_09830"
     CDS             complement(3758..4321)
                     /locus_tag="PKV97_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNB06215.1"
                     /translation="MALPQPAARFERADFFAWEAEQPMKHDYVAGEVFAQAGARQDHV
                     VVALNIATSLRQKLRGTPCRAYISDMQLEVVQADAVFYPDVMVSCHPEDLAAERVLHH
                     PRVIIEVLSDGTAAYDRGAKFAAVRMLDSLQEYALVDPERRSVEIFRRLPSNDWLLAT
                     GDSARGLVLAALELEIGLDEVFEDVGA"
     gene            4491..5060
                     /locus_tag="PKV97_09835"
     CDS             4491..5060
                     /locus_tag="PKV97_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNB06216.1"
                     /translation="MALPKLKPEFDIDAYMAWEEDQPERHEYLGGEAFAMSGGTDAHY
                     TILGNAYAELRAALAGKPCRAFLSGMKLRVDRADAVFYPDVFVTCDPRDRQPEASLAK
                     QHPALIIEVLSDSTAAFDRGRKFEHYQQIDTLQEYLLIEQDRKHADLFRKNSEGLWVL
                     HPAGEGHVVRLESVGAALALDGLYEDVEL"
     gene            complement(5204..6391)
                     /locus_tag="PKV97_09840"
     CDS             complement(5204..6391)
                     /locus_tag="PKV97_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB06217.1"
                     /translation="MRIALIADPFPPLRTSGAVQLRDLAREFVRQGHELTVLLPSSEI
                     AGQWAMEDFDGARVIRLKAPKTKDIGYVRRTIGEFLMPFAMLRNLRRSPLAGERWDGV
                     VWYAPTIFLGPIASALKKASGCRGYLIIRDIFPEWAVDMGLMGRGLPYRFFDAVARYQ
                     YSVADVIGVQTPGNKGYFAEWIQRPGRRLEVLQNWLGAPANAPSTIVVGETELAGRKV
                     FVYAGNMGVAQGMGILLDLAERLRGREDVGFLFVGRGSDAQRLRDDARARGLGNLVFR
                     DEIHPDEIPNLYAQCHIGLVALDPRHRTHNIPGKFLTYMQSGLPVLASVNAGNDLVGL
                     IEQERVGRAVTDASAESLARAAVDLVDAVSADDGFAERCRGLSARLFSAETAVRQIVA
                     ALA"
BASE COUNT         1004 a         2201 c         2145 g         1011 t
ORIGIN      
        1 tggaccgacc gcgatccgcc tcggagtcgt gcatcgcaac tcgcgtcgct cctacgcgcg
       61 cttcagatcc cgcaggcggg ggttgttgac gtccttcgcc gacagctgcg gttccgtgat
      121 cggccacggg atccccacgt ccgggtcgtt ccatgccaga ccgccctcgt ccgccgggaa
      181 gtagaaatcc gtgcacttgt actggaagtc cgccacctcg ctgagcacgc agaacccgtg
      241 cgcgtagccc ggtggcaccc acatctggcg gtggttgtcg tcgttgagct cgacgccgac
      301 gaacttgcca tacgtcgccg agctcgggtc gatgtccacc gcgacgtcat acaccgcccc
      361 gcggctcacg ctcaccagct tgccctgcgg gcgggtcttc tggaaatgca gcccgcgcag
      421 cacgccgcgc tgcgagcgcg agtggttgtc ctgcacgaag ggcagggtga tgccggcctc
      481 gcggtagcgc tcgacctgga aggtctcgag gaagaagccg cggtggtcgc cgaaggcctt
      541 cggctcgatg atcagcaccc cgggcagtgc ggtctcgatc accttcattg cccgccctcc
      601 tggcgcagca ggcgcttgag atactggccg tagccggtct tgctcagcgc ttcggcctgg
      661 cgggccagtt gcgcggccga cagccagccc tggttgtagg cgatctcctc caggcaggcc
      721 accttcaggc cctggcgcgc ctcgatggtc tgcacgaagt ggctcgcctc catcagcgaa
      781 tcgtgcgtgc ccgtgtccag ccaggcgaaa ccgcgtccga gcaggctcac gtgcagatcg
      841 ccgcgctgca ggtaggcgtt gttgacgtcg gtgatctcga gctcgccgcg tgccgagggc
      901 ttcacctgcc tggcgatctc caccaccgag ttgtcgtaga agtacagccc ggtgaccgcg
      961 tagttcgact tgggcgcctt gggcttctcc tcgatcgaga tcgccctgcc gctggcgtcg
     1021 aactcgacca cgccgaagcg ctcggggtcg gagacgtggt agccgaacac cgtcgccccg
     1081 cgctcgcggc gcgccgcctc gcgcagcatg gcgctgaagc cgtagccgta gaagatgttg
     1141 tcacccagga tcaggcagac gtggtcgtcg ccgatgaaat cctcgccgat caggaaggcc
     1201 tgcgccaggc cgtcgggcga gggctgctcg gcgtagctca gcgcgatgcc gaagtccgag
     1261 ccgtcgccga gcagcttgcg aaagttgggc aggtcgtccg gggtcgagat caccaggatc
     1321 tcgcggatgc cggcgagcat cagcacggag agcgggtagt agatcatcgg cttgtcgtag
     1381 atcggcagca gctgcttgga gacgccgcgc gtgatcggat gcagccgggt gccggagccg
     1441 ccggcgagga tgatgccttt catggttcga aggtcctggg tgagagggag cggacggtct
     1501 ggtgacgaag tgcgtgcggc gatgaggggc gagtcgtttc cggttggaag tctacgccat
     1561 cgagccgccg gcgtgaccgt cggagggcgc gatgcacgtc gtgcgtgcgg ggagccatcg
     1621 atgcggtcct tgcgtggtca ggcgcccgaa tcgcgacttg cgtcgctcct acgggaaccg
     1681 gcgcgttcat tggcgctctg cttggcttgt aggagcgacg caagtcgcga tcgcggcgtt
     1741 cggatgtccg cagcggatcg gtgtctggac ggcccatcgc gacttgcgtc gctcctacgc
     1801 ggaggcggcg atgcgggcga ggcaggcatc gcacagggcg cgggcgcggg gctcgtcggc
     1861 ggcttcggcg tagcagcgca gctcgggggc gttgccggag gggcgcaggt gcacgatctc
     1921 gccggtggcg aagctcgtgc gcaagccgtc ggtggtatcg atcgcgatca cgtcgccggc
     1981 gtccggcgcc atcagcgcgg ccgcctccgc gggggtctgc tgcaggcgcg cgatcagggc
     2041 gcggctgaac tcggtcggca cgttctggat gcggtcgctc gcggtgaagc ggcgcggcag
     2101 ggcatcggcc agctgcgaca ctggacagcc cttggcgcgc gccatggcca gcagggccag
     2161 catcggcagc agcgaatcgc gcgtgggcag ggccttcagc cgcgccgcgc cggcgggcaa
     2221 gcatttctgc gtcgcctcgg gcccatccgt cgcaacttcg gcagatccaa agccggtaga
     2281 tccaggctcg gcagatccaa agccgcccgt cccaacgctg cccgccccaa cgccggcggt
     2341 ttccgccgca cttgccccga tcgcctcccc gtactcgaca tcgctcccga gcaggaatcc
     2401 cccgttcgcc tcgtagccca ccacccgccc cgcgccgcgc tgcgtgtcca ttccggcgat
     2461 cacgtagggc gagccgatgc gcgtccgcag cacctgccgg aacgcgccgc acttctccac
     2521 tgcggtgttg ctgctcaccg gcgtcaccac cacgtcggcg tgcagatact gcgcgcacag
     2581 gatgcccacc acgtcgccgc gcagccaggt gccggtctcg tcgccgatca gcgggcggtc
     2641 ggcatcgccg tcggtcgaca ggatggcgtc gaagcgatgc gcctgcgccc atgcgcgcgc
     2701 ctgcgcgatg tcctcggcgc gcacggcctc ggtgtcgatc ggcacgaagg tgtcggtgcg
     2761 cccgagcgac aggacctcgg cgccgaggcg ctcgaacacc gtgcgcagca cgtcgcgcgc
     2821 cacgctcgag tgctcgtaca gtgccacgcg catcccggcg agcgcgcctt cgccgaagaa
     2881 ggcggtgtag cgcgccacgt aggcctccag cgcggaggcg tccaccgcgg gcaggggcgc
     2941 cggcgacagc gcatcgggca cagccacctc cgcatcgagg atcgcctgct cgtccgcctt
     3001 gtcgatctcg ccgtcggcgc gatagaactt gatgccgttg cggtcgaagg ggatgtggct
     3061 gccggtgacg acgatgcccg gagcgcgctg cagcgcgcag taataggcga gcgcgggcgt
     3121 gggcagggcg ccgacgaaca ccgcctcgcg tcccgcgtcg cggatcgcct ggatgcaggc
     3181 cgcggcgatg cgcgggctgc tcgggcgcag gtcgtggccg acgacgacgc gcgcgctgtc
     3241 ggtcgcaatg ctgcgcagga aggcctgcgc gtaggcgtag cagaccgcgt cggagaggtc
     3301 gctgacgagg ccgcgcacgc cgctggtgcc gaagcgcgcg ttgtgggcct cggcgaggtg
     3361 gtggagtcgt gtgttcatcg gatgtcggtg aatcggggga tggagaaagt ggatgtcgcc
     3421 cgcgcgtgcc gatcgtgcgg cagccttccg taggnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnatggag aaagtggatg tcgcccgcgc gtgccgatcg tgcggcagcc
     3601 ttccgtaggg tccttatgta ggagcgacgc aagtcgcgat ccggcgatcc ggcgatccgg
     3661 ttgctacggg cgtatttcag atcccggacg cttggatcgc gacttgcgtc gctcctacaa
     3721 aaatcaaaac cgggagcgcc gtcccggcgg tcacatttca ggcgccgacg tcctcgaaca
     3781 cctcgtccag cccgatctcc agctccagtg ccgccagcac caggccgcgc gcgctgtcgc
     3841 cggtggcgag cagccagtcg ttggagggca ggcggcggaa gatctcgacg ctgcgtcgct
     3901 cgggatcgac cagggcgtac tcctgcaggc tgtcgagcat gcgaaccgcg gcgaacttcg
     3961 caccgcggtc gtaggcggcg gtgccgtcgc tcaacacctc gatgatcacg cgcggatgat
     4021 gcagcacgcg ctcggcggcg aggtcttcgg ggtggcagct caccatcacg tcggggtaga
     4081 acaccgcatc ggcctgcacc acctcgagct gcatgtcgga gatgtaggcc cggcagggcg
     4141 tgccgcgcag cttctggcgc agcgaggtgg cgatgttgag tgccacgacc acatgatcct
     4201 gccgtgctcc ggcctgggcg aacacctcgc cggcgacgta gtcgtgcttc atgggctgtt
     4261 cggcctccca ggcgaagaaa tccgcgcgtt cgaagcgcgc ggcgggttga ggcaaggcca
     4321 tgatgggcga gctcccgatc cgtgagatgg ctgaagtcta gcagggtgag ggcgggctgc
     4381 tcgatgggcg ccgcgggccg cggcgcggcc gtgagcaaat gaaagacctg accccggttt
     4441 tctttcccaa atcgacccgc tatgctcgac caaacgaagg agttccgact atggccctgc
     4501 ccaagctcaa acccgaattc gacatcgacg cctacatggc gtgggaagag gatcagcccg
     4561 aacgtcacga gtacctcggc ggcgaggcct tcgccatgtc cggaggcacg gatgcgcatt
     4621 acacgatcct cggcaatgct tatgccgaac tccgtgccgc acttgccggc aaaccctgcc
     4681 gcgccttcct ctccggcatg aagctgcgcg tcgaccgtgc cgacgccgtc ttctaccccg
     4741 atgtcttcgt cacctgcgac ccgcgcgacc gccagcccga ggccagcctg gccaagcagc
     4801 acccggcgct gatcatcgag gtcctctccg actccaccgc cgccttcgac cgcggtcgca
     4861 agttcgagca ctaccaacag atcgacaccc tgcaggaata tctgctgatc gagcaggacc
     4921 gcaagcacgc cgaccttttc cgcaagaaca gcgaagggct ttgggtgctg catccggcgg
     4981 gagaggggca tgtggtgagg ctggaaagcg tcggggctgc actggcactt gacggtctgt
     5041 acgaggacgt cgagctctga gccgcattca cgcctcgcgg cgctccacat gcgcttgccg
     5101 ttgcgctggt tcatgtagga gcgccgcaag gcgcgaatgc ggcggtcgaa cgcgcgtggt
     5161 gcgtatcgga agcgcaccat tcgcggcttg cgccgctcct acatcaggcc aaggcagcca
     5221 cgatctggcg gacggcggtt tcggcggaga acaggcgcgc ggacaagccc ctgcagcgct
     5281 cggcaaaccc atcgtccgcc gacaccgcat ccaccagatc caccgccgcc cgcgccagcg
     5341 actccgccga cgcgtcggtc accgcccgcc cgacgcgctc ctgctcgatc aagcccacca
     5401 ggtcgttccc cgcattcacg ctcgccagca ccggcaagcc gctctgcatg taggtgagga
     5461 acttgcccgg gatgttgtgc gtgcggtggc gcgggtcgag cgcgaccagg ccgatgtggc
     5521 actgcgcata caggttcggg atctcgtccg gatggatctc gtcgcgaaac accaagttgc
     5581 ccagcccgcg tgcgcgcgca tcgtcgcgca ggcgctgggc gtcgctgccg cggcccacga
     5641 acagaaagcc cacatcctcc cgcccgcgca ggcgctcggc caggtcgagc aggatgccca
     5701 tgccctgcgc cacgcccatg ttgcctgcgt acacgaacac cttgcgcccg gccagctccg
     5761 tctcgcccac cacgatcgtg ctcggcgcat tggccggcgc gcccagccag ttctgcagca
     5821 cctccagccg acggcccggg cgctggatcc actccgcgaa gtagcccttg ttgcccggcg
     5881 tctgcacccc gatcacatcc gccaccgaat actggtagcg cgccaccgca tcgaagaagc
     5941 gatacggcag cccgcgcccc atcagcccca tgtccaccgc ccactcggga aagatgtcgc
     6001 ggatgatcag gtagccgcgg cagccgctcg ccttcttcag cgcgctcgcg atcggcccca
     6061 ggaagatcgt cggcgcgtac cacaccacgc cgtcccacct ctccccggcc agcggactgc
     6121 gccgcaggtt gcgcagcatc gcgaacggca tcaggaactc gccgatcgtg cgccgcacat
     6181 agccgatgtc cttggtcttc ggcgccttca gccggatcac gcgcgcgcca tcgaaatcct
     6241 ccatcgccca ctggccggcg atctcggacg acggcagcag caccgtcagc tcatgcccct
     6301 ggcgcacgaa ctcgcgcgcc aggtcgcgca actgcaccgc gcccgaggtg cgcagcggcg
     6361 ggaaggggtc ggcaatcaga gcaatccgca tcgcctcaat attccttcca caccacccgc
     6421 ttcacgtaat ccgtgtagct gtggatgatg cgcaccacct t
//
LOCUS       DAOQNN010000166         6458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6719_length_6458_cov_9.190536, whole genome shotgun sequence.
ACCESSION   DAOQNN010000166 DAOQNN010000000
VERSION     DAOQNN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6719_length_6458_cov_9.190536"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PKV97_09845"
     CDS             complement(<1..464)
                     /locus_tag="PKV97_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB06218.1"
                     /translation="MAVYDLEEQEQISELKAWWAQYGNFVVTLAVVAALASVGWQAWN
                     WYQSRNAGEAGALYYAVQQAVEKNEPQKAREAAGRLVSEHGGSVGAQLGALLSAAMQF
                     ESGDLANARAQLEWAADKGSDPALRELARLRLAAVLLQAGELDAALARLQAAP"
     gene            complement(467..1762)
                     /gene="hisS"
                     /locus_tag="PKV97_09850"
     CDS             complement(467..1762)
                     /gene="hisS"
                     /locus_tag="PKV97_09850"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNB06219.1"
                     /translation="MSQTLQAVRGMNDILPADAETWEYFEDIVRDWLQSYGYRPIRMP
                     LVEPTPLFKRAIGEVTDIVEKEMYSFEDALNGEHLTLRPEGTASCVRAAIQHNLIPAG
                     GPQRLYYYGPMFRHERPQKGRYRQFHQIGVEALGFAGADTDAELILMCARLWEDLGLE
                     DVALEINSLGSPEERAQHRAALIAHLEQHQDKLDEDGKRRLYTNPLRILDTKNPELQA
                     IVEAAPRLADYLGDESKAHFEAVQVFLKDAGIPYRINHRLVRGLDYYNRTVFEWVTTR
                     LGAQGTICAGGRYDGLFEQLGGKPQPAAGFAIGIERLLLLWQACGGEAERPVPDVYVV
                     SVGEAAQRLGFRAAETLREHGFAVLMHCGGGSFKSQMKKADASEAPVAIVIGEDEAAA
                     GEVGLKPLRVAGAQQRVAIDDLVEAMAALMFPEEEDEEV"
     gene            complement(1820..3079)
                     /gene="ispG"
                     /locus_tag="PKV97_09855"
                     /gene_synonym="gcpE"
     CDS             complement(1820..3079)
                     /gene="ispG"
                     /locus_tag="PKV97_09855"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNB06220.1"
                     /translation="MNPRHELQPIEARPLARHRTHQVRVGKVRIGGEAPVVVQSMTNT
                     DTADVLATAMQVAELARAGSEIVRITVNNEAAAAAVPKIRDRLLALNMDVPLVGDFHY
                     NGHKLLTDFPACAEALAKLRINPGNVGAGRKRDPQFAAIVELACRYDKPVRIGVNWGS
                     LDQSVLARIMDANAKRAEPRDAGAVMREALVVSALESAAKAEEYGLGRERIILSAKVS
                     SVQDLIAVYRDLARRSDYALHLGLTEAGMGSKGIVGSTAALAVLLQEGIGDTIRISLT
                     PEPGGSRTQEVVVAQEILQTMGLRAFTPMVTACPGCGRTTSTFFQELASGIQDYVRAQ
                     MPVWREQYDGVENMTLAVMGCVVNGPGESKHANIGISLPGTGETPAAPVFVDGEKVVT
                     LRGDNIAAEFKALVDDYVATRYVKKGA"
     gene            complement(3090..4058)
                     /locus_tag="PKV97_09860"
     CDS             complement(3090..4058)
                     /locus_tag="PKV97_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNB06221.1"
                     /translation="MQSDHLALGAESAASPGARLRRAREARGESVHEAAFAIKLSPRQ
                     IEALENDDFAALPGMAFVRGFARNYARYLGLDATPLLDGIERLAGAGQPDLSPIRNAD
                     GDLPAGGGGRRGSFPAGVVVALLLVLLGVGWYFDWFRTEPLPGLELQTEPAPAFMPAP
                     TEGGGAAPATSEPVATPAPLEASAPAAPHASPVAIPAAVGAGVPVTPLAAPASATPPA
                     SASAIVSPVVQSPAQAATPVAEEGAGGNRLSFRFGGDSWIEVRDASGTILHSGTNRAG
                     SSRVVQGTPPFRLVVGNSANVVLEQDGKAVDLAAHTRGSVARLTLP"
     gene            complement(4064..4864)
                     /gene="pilW"
                     /locus_tag="PKV97_09865"
     CDS             complement(4064..4864)
                     /gene="pilW"
                     /locus_tag="PKV97_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HNB06222.1"
                     /translation="MKSTLAPVVLAVALVLGGCAGAPGVPAGSSSISRPMSDTNPATP
                     ADARARVHVELGMAYFEVGRYDVALDEAKIALADSPSYAPAYHLLGLAYMSVEEHAAA
                     RENFERALREAPGDPDFNNSYGWFLCSQGQERAGLEYLALAARNPYYRHQTRPLTNAG
                     LCHLRLGEEAAAEAQFQRAVQADPENAQALYLLADIAYRGGRYEQARSELIRLHQAHG
                     PSAASAWLGLRTERRLGNADAEASYAAQLRSRFAESEEFKSMNQGKFE"
     gene            complement(4861..6009)
                     /gene="rlmN"
                     /locus_tag="PKV97_09870"
     CDS             complement(4861..6009)
                     /gene="rlmN"
                     /locus_tag="PKV97_09870"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNB06223.1"
                     /translation="MSTPNPVNLLDFDVDGLVAWFAGLGEKPFRARQVMRWMHHEGCD
                     DFDAMTDVAKSLRAKLKDLAVIRPPVPVRDSISADGTRKWLLDVGNANAVETVFIPET
                     SRGTLCVSSQAGCALDCAFCSTGKQGFNRNLSAAEIIGQLWLANKLLGAARADAEEHA
                     TDLEAGEKDNGRIISNVVMMGMGEPLANFDNVVTALRLMLDDHAYGLSRRRVTVSTSG
                     IVPAMDRLRDECPVALAVSLHASNDALRDRLVPINQKYPLRELMAACQRYLERAPRDF
                     VTFEYVMLEGVNDSDAHARELVALVRDTPCKFNLIPFNPFPDSGFQRSPAERIRRFAG
                     ILIDAGIVTTTRKTRGDDVDAACGQLAGQVQDRTRRTVRLHQARENMR"
     gene            complement(6142..>6458)
                     /locus_tag="PKV97_09875"
     CDS             complement(6142..>6458)
                     /locus_tag="PKV97_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNB06224.1"
                     /translation="KMAHLSEREAGEFYAVHKERPFFKDLVSFMTSGPVMIQCLEGEN
                     AIAKNRELMGATDPKKADKGTIRADFAESIDANAVHGSDAPETAAVEVAFFFPGMNVY
                     SR"
BASE COUNT          913 a         2196 c         2331 g         1018 t
ORIGIN      
        1 ggcgcggcct gcaggcgggc gagcgcggcg tcgagttcgc ccgcctgcag cagcaccgcc
       61 gccagacgca ggcgtgcgag ctcgcgcagc gcggggtcgc tgcccttgtc ggccgcccac
      121 tcgagctgcg cgcgcgcgtt ggcgaggtcg ccggactcga actgcatcgc cgccgacagc
      181 agcgcgccga gttgggcgcc tacactgccg ccgtgctcgc tcaccaggcg accggccgcc
      241 tcgcgggcct tctgcggctc gttcttctcc accgcctgct gcaccgcgta gtacagcgca
      301 ccggcctcgc cggcattgcg gctctggtac cagttccagg cctgccagcc caccgaggcg
      361 agcgccgcca ccaccgcgag ggtgacgacg aagttgccgt actgcgccca ccacgccttg
      421 agttcggaaa tctgttcctg ctcttcgaga tcgtagaccg ccatcatcaa acctcttcgt
      481 cttcttcttc ggggaacatc agggcggcca tcgcctcgac caggtcgtcg atcgccacgc
      541 gctgctgggc gcccgcgacg cgcaggggct tgaggccgac ctcccccgcc gcggcctcgt
      601 cctctccgat cacgatcgcc accggcgcct cgctggcgtc ggccttcttc atctgcgact
      661 tgaagctgcc gccaccgcaa tgcatcagca ccgcgaagcc gtgctcgcgc aaggtctcgg
      721 cggcgcggaa accgaggcgc tgcgcggcct cgcccacgct caccacatac acgtcgggca
      781 ccggacgctc ggcctcgcca ccgcaggcct gccacagcag cagcaggcgc tcgatgccga
      841 tcgcgaagcc cgcggccggc tgcggcttgc cgccgagctg ctcgaacagg ccgtcgtagc
      901 gcccgccggc gcagatcgtg ccctgcgcgc ccaggcgcgt ggtgacccac tcgaacaccg
      961 tgcggttgta gtagtccagg ccgcgcacca ggcggtggtt gatgcgatac gggatgccgg
     1021 cgtccttgag gaagacctgc accgcctcga agtgcgcctt cgattcgtcg ccgagatagt
     1081 cggcgagcct gggcgcggct tcgacgatcg cctgcagttc gggattcttg gtgtcgagga
     1141 tgcgcagcgg gttggtgtac aggcggcgct tgccgtcctc gtcgagcttg tcctgatgct
     1201 gctcgaggtg ggcgatcagc gcggcgcggt gctgcgcgcg ctcctcgggc gagccgagcg
     1261 agttgatctc gagcgcgaca tcctccaggc cgaggtcctc ccacagccgc gcacacatca
     1321 ggatcagctc ggcgtcggta tcggctccgg cgaagccgag tgcctccacg ccgatctggt
     1381 ggaactggcg gtagcggccc ttctgcggac gctcgtggcg gaacatcggg ccgtagtagt
     1441 acagccgctg cgggccgccc gcggggatca ggttgtgctg gatcgcggcg cgcacgcagg
     1501 aggccgtgcc ttcggggcgc agcgtgaggt gctcgccgtt gagcgcgtcc tcgaaggagt
     1561 acatctcctt ctcgacgatg tcggtgacct cgccgatggc gcgcttgaac agcggcgtcg
     1621 gctcgaccag cggcatgcgg atcgggcggt aaccgtagct ctgcagccag tcgcgcacga
     1681 tgtcttcgaa gtattcccag gtttcggcgt cggccggcag gatgtcgttc atcccgcgca
     1741 cggcctgcaa ggtctggctc atgtggttct tttcggttgt gccgcgctca cgcgcggcgg
     1801 tcgttgttcg ttgcggcgct caggcgccct tcttcacgta gcgggtggcg acgtaatcgt
     1861 cgacgagcgc cttgaactct gcagcgatgt tgtcgccgcg cagggtgacg accttctcgc
     1921 cgtcgacaaa caccggcgcc gccggggttt cgccggtgcc cggcagcgag atgccgatgt
     1981 tggcgtgctt gctctcgccc gggccgttga ccacgcagcc catcaccgcc agcgtcatgt
     2041 tctcgacgcc gtcgtactgc tcgcgccaca ccggcatctg cgcgcgcacg tagtcctgga
     2101 taccggaggc gagctcctgg aagaaggtgc tggtggtgcg gccgcagccc gggcaggcag
     2161 tcaccatggg ggtgaaggcg cgcaggccca tggtctgcag gatctcctgc gcgaccacga
     2221 cctcctgggt gcggctgccg cccggctcgg gggtgagcga gatgcggatg gtgtcgccga
     2281 tgccttcctg cagcagcacg gcgagcgcgg cggtggagcc gacgatgccc ttgctgccca
     2341 tgccggcctc ggtgaggccc agatgcagcg cgtagtcgct gcgccgggcg agatcgcggt
     2401 acaccgcgat caggtcctgc acgctggaaa ccttggccga caggatgatg cgctcgcggc
     2461 cgaggccgta ttcctcggcc ttggccgcgg attcgagcgc cgagacgacg agcgcctcgc
     2521 gcatcaccgc gccggcgtcg cgcggctcgg cacgcttggc attggcgtcc atgatgcggg
     2581 caaggaccga ctggtcgagg ctgccccagt tcacgccgat gcgcaccggc ttgtcgtagc
     2641 ggcaggcaag ctcgacgatc gcggcgaact gcgggtcgcg cttgcggccg gcgccgacgt
     2701 tgcccgggtt gatgcgcagc ttggcgagcg cctcggcgca cgccgggaag tcggtgagca
     2761 gcttgtggcc gttgtagtgg aagtcgccga ccagcggcac gtccatgttg agcgcgagca
     2821 ggcggtcgcg gatcttcggc accgccgccg ccgcggcctc gttgttgacc gtgatgcgca
     2881 cgatctcgga gccggcgcgg gcgagctcgg cgacctgcat cgcggtggcg agcacgtcgg
     2941 ccgtgtcggt attggtcatc gactgcacga cgaccggggc ttcgccgccg atcctcacct
     3001 tgccgacgcg cacctgatgg gtgcgatgac gggcgagcgg gcgggcttcg atcggctgga
     3061 gttcgtgtcg gggattcatg gcgttcggat cagggaagcg tcaggcgggc gacggagccg
     3121 cgggtgtggg ctgcgagatc gaccgccttg ccgtcctgct cgagcacgac gttggcggag
     3181 ttgcccacca ccaggcggaa gggcggcgtg ccctggacca cccggctgct gccggcacgg
     3241 ttggtgccgg agtgcaggat ggtgccgctg gcgtcgcgca cctcgatcca ggagtcgcct
     3301 ccgaagcgga agctgaggcg gttgccgccg gcaccttcct cggcgacggg ggtggccgcc
     3361 tgggccggcg actgcaccac gggagacacg atggccgagg ctgaggccgg gggcgttgcg
     3421 ctcgccggcg cggcgagcgg ggtcaccggg acgcctgcac ccaccgcagc gggaatcgcc
     3481 accgggctgg cgtgcggcgc cgcgggcgcg ctcgcctcca gcggagcggg cgtggccacc
     3541 ggctcgctcg tggccggcgc ggcgccgcca ccctcggtgg gcgcgggcat gaaggcgggc
     3601 gcaggctcgg tctgcagttc gagcccgggc aggggctcgg tgcggaacca gtcgaagtac
     3661 cagcccacgc cgagcaagac cagcaacaag gccacgacga ccccagccgg gaagctgccg
     3721 cgacgcccgc cgccaccggc gggcaggtcg ccatcggcat tgcggatcgg cgacaggtcg
     3781 ggctgcccgg cgccggccag gcgctcgatg ccatcgagca gcggcgtcgc gtcgagtccg
     3841 aggtaacgcg cgtagttgcg cgcgaaacca cgcacgaagg ccatgcccgg gagcgcggcg
     3901 aagtcgtcgt tttccagcgc ctcgatctgg cgcgggctca gcttgatggc gaaagcggcc
     3961 tcgtgcaccg actcgccacg cgcctcgcgc gcccggcgca ggcgggcgcc gggcgaggcg
     4021 gcggactcgg cgccaagtgc aaggtggtcg gactgcatgc tgctcactcg aacttccctt
     4081 gattcatcga tttgaactcc tccgattcgg cgaagcggct gcgcaactgg gcagcatagc
     4141 tcgcctcggc gtcggcgttg ccgaggcggc gctcggtgcg caggcccaac caggccgaag
     4201 ccgcgctcgg tccgtgcgcc tggtgcagcc ggatcagctc gctgcgcgcc tgctcgtagc
     4261 gaccgccgcg ataggcgatg tccgcgagca ggtagagtgc ctgcgcgttc tccgggtcgg
     4321 cctgcaccgc gcgctggaat tgcgcctcgg ctgcagcctc ctcgccgagg cgcagatggc
     4381 acaggcccgc gttggtgagc gggcgcgtct ggtggcggta atacgggttg cgcgccgcga
     4441 gcgcgaggta ctcgaggcct gcgcgctcct ggccctgaga gcacaggaac cagccgtagc
     4501 tgttgttgaa gtcggggtca cccggtgcct cgcgcagcgc acgctcgaag ttctcgcgcg
     4561 cggcggcgtg ctcctcgacg gacatgtagg ccagcccgag caggtggtag gccggggcgt
     4621 aggacgggct gtcggcgagc gcgatcttcg cctcgtcgag cgcgacgtcg tagcgcccca
     4681 cctcgaagta ggccatgccg agctccacat gcacgcgtgc gcgcgcatcg gccggcgtcg
     4741 ccgggttggt gtcggacatc ggccggctga tcgaggacga cccggcgggt acgccgggcg
     4801 cacccgcgca gccgccgagc acgagcgcca ccgccagcac gaccggtgcc agtgtcgatt
     4861 tcatcgcatg ttctccctcg cctggtgcag gcgcacggta cgccgcgtcc tgtcctggac
     4921 ctggccggcg agctggccgc acgcagcgtc gacatcgtcg ccacgcgtct tgcgcgtggt
     4981 ggtcacgata ccggcgtcga tcaggatacc ggcaaaacgc cggatacgct ccgcgggcga
     5041 gcgctggaag ccggagtccg ggaaggggtt gaacgggatc aggttgaact tgcacggcgt
     5101 gtcgcgcacc agcgcgacga gttcgcgagc gtgcgcgtcg ctgtcgttga cgccttcgag
     5161 catcacgtac tcgaaggtga cgaagtcgcg cggcgcgcgc tcgaggtagc gctggcaggc
     5221 ggccatgagc tcgcgcagcg ggtacttctg attgatcggc acgaggcggt cgcgcagcgc
     5281 gtcgttggac gcatgcagcg acaccgccag cgccaccgga cattcgtcgc gcaggcggtc
     5341 catcgccggc acgatgcccg aggtcgacac cgtgacccgg cggcgcgaca ggccgtaggc
     5401 gtggtcgtcc agcatgaggc gcagcgcggt gacgacgttg tcgaagttgg cgagcggctc
     5461 gcccatgccc atcatcacca cgttgctgat gatgcggccg ttgtccttct cgccggcctc
     5521 gaggtccgtg gcgtgctcct cggcgtcggc ccgcgccgcg ccgagcagct tgttggccag
     5581 ccagagctgg ccgatgattt ccgcggccga caggttgcgg ttgaagccct gcttgccggt
     5641 ggagcagaac gcgcagtcga gcgcgcagcc cgcctgcgag gacacgcaca gcgtgccgcg
     5701 gctggtctcg ggaatgaaca cggtctcgac cgcgttggcg ttgcccacgt cgagcagcca
     5761 cttgcgcgtg ccgtcggccg agatcgagtc gcgcaccggc accggcgggc ggatcacggc
     5821 gaggtccttc agcttcgcgc gcagggactt ggcgacgtcg gtcatggcgt cgaagtcgtc
     5881 gcagccctcg tgatgcatcc agcgcatcac ctggcgggcg cggaacggct tctcgcccag
     5941 cccggcgaac caggcgacga ggccgtctac gtcgaaatcg agcagattga ccggattggg
     6001 agtgctcatg cctgaaactt cacctctcgg gagcggaggc ccgcgatgac gggccgaccg
     6061 taatgcaaac ggcccgctca cgccgcgcgc ggtgcgggag gcaccgcgaa gcgtgcgcga
     6121 cgggccgctg gtacggaccg ctcagcgcga gtaaacgttc atgcccggga agaagaaagc
     6181 cacttccacg gcagcggtct cgggggcgtc ggagccgtgc acggcgttgg cgtcgatcga
     6241 ctcggcgaag tcggcgcgga tggtgccctt gtcggccttc ttcgggtcag tggcgcccat
     6301 cagctcgcgg ttcttggcga tggcgttctc gccttccagg cactggatca tcaccgggcc
     6361 ggaggtcatg aaggagacca gatccttgaa gaaggggcgc tctttgtgca cggcgtagaa
     6421 ctcaccggct tcacgctcgg acaggtgagc catcttgg
//
LOCUS       DAOQNN010000167         6432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6763_length_6432_cov_8.909989, whole genome shotgun sequence.
ACCESSION   DAOQNN010000167 DAOQNN010000000
VERSION     DAOQNN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6763_length_6432_cov_8.909989"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(482..1936)
                     /locus_tag="PKV97_09880"
     CDS             complement(482..1936)
                     /locus_tag="PKV97_09880"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNB06225.1"
                     /translation="MSSALVWFRRDLRSFDHAALHHALRFAERVHCAFVFDTAILDAL
                     PARADRRVEFIHASLAELDAALDAMARAAGGTGGGLIVRHGRAEDEIPRLAAELGVDK
                     VFANRDYEPDAILRDSRVAQRLAEDGIGYEDFKDQVVFERDEVLTQTGKPYSVFTPYK
                     NAWLKAVDDFQVSSYAVEKYAARLAPKPAGETLPGLADIGFAPTNLAELNMPVGMSGG
                     KRLFDDFAARIERYKRARDFPAVKGVSYLSTHLRFGTVSIRQLAAHARARGGEGAETW
                     LSELIWRDFYHQILWHHPQVVDRAFRPEYDAVQWNDAPELFAAWCEARTGYPIVDAAM
                     RQLDQTGWMHNRLRMIVASFLTKDLGVDWRLGERYFAIRLNDYDLAANNGGWQWAAST
                     GCDAQPWFRIFNPVTQSEKFDPEGRFIRRYLPELARVPDKFIHAPWTMGGIDQTACRT
                     KIGVDYPGPVVDHAAARERTLKRFGVVKNDAQGA"
     gene            2223..>2385
                     /locus_tag="PKV97_09885"
     CDS             2223..>2385
                     /locus_tag="PKV97_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNB06226.1"
                     /translation="METPTANFTHHFLIAMPNMVDPHFARTLTYIAEHNDQGALGIIV
                     NRPIDMTLAS"
     assembly_gap    2386..2485
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2486..2944
                     /locus_tag="PKV97_09890"
     CDS             <2486..2944
                     /locus_tag="PKV97_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNB06227.1"
                     /translation="QGALGIIVNRPIDMTLASLFERIEVPLEAEGFAAQPVYFGGPVQ
                     TDRGFVLHRPLGDWHSTLKVTDEIGLTSSRDVLQAIGAGGEPRDVIVTLGYAGWAAGQ
                     LEQELLDNAWLTVPADLEIVFELPPEERLVAAMQKLGIDFTNLSDVAGHA"
     gene            2925..3443
                     /gene="ruvX"
                     /locus_tag="PKV97_09895"
     CDS             2925..3443
                     /gene="ruvX"
                     /locus_tag="PKV97_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNB06228.1"
                     /translation="MSPDMPEAATAAPSPADTRFPRPAPDTLPARGTVLGFDFGLARI
                     GVASGELETGLASALTTVHAEANDARFAAIARLIAEWRPVALVVGIPTHLDGREHELT
                     ARCRRFANQLHGRHALPVFTADERLSSVAAEAELAEAGRKDWRERKAVLDAAAARILL
                     QTFLDTRRHERP"
     gene            3430..3933
                     /gene="pyrR"
                     /locus_tag="PKV97_09900"
     CDS             3430..3933
                     /gene="pyrR"
                     /locus_tag="PKV97_09900"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNB06229.1"
                     /translation="MKDLDAEALCRQLAEQIRPHVGPATALVGIYTGGLWLAERLHAE
                     LGIAQPLGAIDVSFYRDDYASKGLHASPKKTEIPFDVDGAHVILVDDVLYTGRTTRAA
                     LNELFDFGRPARVELAVLVDRGGRELPVAARYCAHTLSVPLAREQNLELQRDEAGHLG
                     LELTRRA"
     gene            3988..4947
                     /locus_tag="PKV97_09905"
     CDS             3988..4947
                     /locus_tag="PKV97_09905"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNB06230.1"
                     /translation="MRNPQLNQHGELQHLLTLDGLPRNILNAILDTAAPFTEVAEREV
                     KKLPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVVNLNINTSSAAKGESLLDTVD
                     NLCAMQADMFVVRHAASGAPMLIAQHLQNTGRDHIHVVNAGDGRHAHPTQGLLDMYTI
                     RHFKKDFTNLTVAIVGDVLHSRVARSQIAALTTLGVPEVRVIGPRTLLPTEVEKLGVR
                     VFHDMREGLKGVDVVMMLRLQNERMNGALLPTPQEYYKIWGLTAEKLALARPDAIVMH
                     PGPMNRGVEIDSAVADGAQAVILPQVTFGIAVRMAVMSMLGSR"
     gene            4981..6258
                     /locus_tag="PKV97_09910"
     CDS             4981..6258
                     /locus_tag="PKV97_09910"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNB06231.1"
                     /translation="MNILISNGRVVDPANRIDAVHNVYVSGGKIVALGQAPDGFVAER
                     TIDAGGLVVAPGFIDLAARLREPGYEYRATLESEMEAAMAGGVTSLAIPPDTDPVLDE
                     PGLVEMLTYRAKKLNRAHIYPVGALTIGLQGERLSEMAELVEAGCVAFSQANVPLVDN
                     TVLMRALQYAATFGFRVWLQPLAPFLSQVGHAHDGEVATRLGLSGIPVAAETVALYTY
                     LELARITGARLHITRLSSAAGLALIDQARAEGMDVTCDVSINHVHLCDMDIGYFNPNC
                     HLVPPLRSQRDREALARGLAEGRIDALCSDHTPVDDDAKQTPFSESEPGATGLELLLP
                     LTLKWADRAGLALLDGLARITSDAAKIVGITKAGHLSVGARADVCVFDPATHVTITRE
                     GLRSQGKNTPFLGMELPGKVRYTLVEGQVMFEG"
BASE COUNT         1001 a         2362 c         2090 g          879 t
ORIGIN      
        1 gtaaagggtg acgggacctc attgtcccgc attcccccgc gcgctcaccg ccccgtagga
       61 gcgacgcaag tcgcgatccg gcgcccgggc tgcgcaccga atcccgggca tggcgcggcg
      121 gcgatgtcgg ggggatggcg cggcggcgat ccggccgcgg gaccggggtg gcgggcgccg
      181 cctgcactcg cggccctgca ctcgcggccc tgcgcttcgc tgcgggctgc cctcggtgga
      241 ggacgtcgcg ggggcggcga cgcaaactcg gcgctgcgcg cctcagacat gcgtcgcctt
      301 gtttcccccg cgacgccctc caccgaggcg ctcctgaagt tggcgtccgc caccccggcc
      361 ccacggcctt gcgtgggaga gtggctgaga ggtcgagggc ggtggggggt gcgtggaacg
      421 gcgcgggcac ttgttcccgg gccactcttg tcagcgggcg gccgccgcct ctggctgacg
      481 cctaggcgcc ttgcgcatcg ttcttcacga ccccgaagcg cttcagcgtg cgttcgcgcg
      541 cggcggcgtg gtccacgacc ggccccgggt agtccacacc gatcttcgtg cggcacgcgg
      601 tctggtcgat gccgcccatg gtccacgggg cgtggatgaa cttgtcgggc acgcgcgcga
      661 gttcgggcag gtagcggcgg atgaagcggc cttcggggtc gaatttttcc gactgggtga
      721 cggggttgaa gatgcggaac cagggctggg cgtcgcagcc ggtcgaggcc gcccactgcc
      781 agccgccgtt gttggcggcg aggtcgtagt cgttgagccg gatggcgaag taacgctcgc
      841 ccaggcgcca gtcgacgccg aggtccttgg tgaggaagga ggcgacgatc atgcgcaggc
      901 ggttgtgcat ccagccagtc tggtcgagct ggcgcatcgc cgcatcgacg atcggatagc
      961 cggtgcgcgc ctcgcaccag gcggcgaaga gctcgggcgc gtcgttccac tgcacggcgt
     1021 cgtactcggg acggaaggca cggtccacca cctgcgggtg gtgccacagg atctggtggt
     1081 agaagtcgcg ccagatcagc tccgacagcc aggtctccgc tccctcgccg ccgcgcgcgc
     1141 gggcgtgggc ggcgagctgg cggatcgaca cggtgccgaa gcgcaggtgg gtggacagat
     1201 agctgacgcc cttcaccgcc gggaagtcgc gcgcgcgctt gtagcgctcg atgcgcgcgg
     1261 cgaagtcgtc gaacaggcgc ttgccgccgc tcatgcccac aggcatgttc agctcggcga
     1321 ggttggtggg cgcgaagccg atgtcggcca gcccgggcag ggtctcgccg gcgggcttcg
     1381 gcgccaggcg cgcggcgtac ttctccaccg catacgagct gacctggaaa tcgtccaccg
     1441 ccttcagcca ggcgttcttg taaggcgtga acacgctgta gggcttgccc gtctgggtga
     1501 gcacctcgtc acgctcgaag accacctggt ccttgaagtc ctcgtaaccg atgccgtcct
     1561 cggcgagtcg ctgcgcgacc cggctgtcgc gcaggatcgc gtcgggttcg tagtcgcggt
     1621 tggcgaagac cttgtcgacg ccaagctcgg cagccaggcg cgggatctcg tcctcggcgc
     1681 ggccatggcg gacgatcagc ccgcctcccg tgccacccgc ggcacgcgcc atggcgtcga
     1741 gcgccgcatc cagctcggcc aggctggcgt ggatgaactc gacgcggcgg tcggcgcgcg
     1801 cgggcagcgc gtcaaggatg gcggtatcga agacgaaggc gcaatgcacg cgctcggcga
     1861 agcgcagggc gtggtggagc gcagcgtgat cgaagctgcg caggtcgcgg cggaaccaga
     1921 cgagggcgga agacattttt tgtccaggac aaagatgcgc aattatgacg catccgccac
     1981 cgtgccgccg cagtccggac aagcgcgagg ctgcagggcc gcatcggagc agcacgcgat
     2041 cgcccacgcc gcacgccaca ccatccactc aggaagggaa tcgacgatgc cggcgaccga
     2101 tcgctgcaat cgcgacttgc gtcgctccca cagaaaccat gcgcggccgc ccgacggcgg
     2161 gagaacccgc tcgcacaagc ccccacgcca agaagaccgg cgttcggcgt agaatgccgc
     2221 gcatggaaac gcccacggcc aacttcacgc accacttcct gatcgccatg cccaacatgg
     2281 tcgatccgca cttcgcgcgc acgctcacct acatcgccga acacaacgac cagggcgcgc
     2341 tcggcatcat cgtgaaccgg ccgatcgaca tgaccctggc cagccnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnncaggg cgcgctcggc atcatcgtga accggccgat
     2521 cgacatgacc ctggccagcc tcttcgagcg catcgaggtg ccgctcgagg ccgagggctt
     2581 cgccgcgcag ccggtgtatt tcggcggccc ggtgcagacc gaccgcggct tcgtgctgca
     2641 ccgcccgctc ggcgactggc actccacgct caaggtcacc gacgagatcg gcctcaccag
     2701 cagccgcgac gtgctgcagg ccatcggcgc cggtggcgaa cccagggacg tcatcgtcac
     2761 cctcggctac gccggctggg ccgccggcca gctcgagcag gagctcctcg acaacgcctg
     2821 gctcaccgtc cccgccgacc tcgagatcgt gttcgagctg ccgcccgagg agcgcctcgt
     2881 cgccgccatg cagaagctcg gcatcgactt caccaacctg agcgatgtcg ccggacatgc
     2941 ctgaggcggc cacggccgcc ccgtcccctg cggacacccg cttcccccgg cccgccccgg
     3001 acacgctccc cgcgcgtggc accgtgctcg gtttcgactt cggcctcgcc cgcatcggcg
     3061 tcgccagcgg cgagctcgag accggcctgg ccagcgcgct caccaccgtg cacgccgagg
     3121 cgaacgacgc acgcttcgcc gccatcgcca ggctgatcgc cgaatggcgg ccggtcgcgc
     3181 tggtggtcgg cattcccacc catctggacg gccgcgagca cgagctcacc gcgcgctgcc
     3241 gccgcttcgc caaccagctc cacggccgcc acgccctgcc ggtgttcacc gccgacgagc
     3301 gcctgtcctc ggtcgccgcc gaagccgagc tcgccgaggc cggccgcaag gactggcgcg
     3361 agcgcaaggc ggtgctcgac gccgccgccg cccgcatcct cctgcaaacc ttcctggaca
     3421 cacgacgcca tgaaagacct tgacgccgaa gccctgtgcc ggcagctcgc cgagcagatc
     3481 cgcccccacg tcggccccgc caccgcgctc gtcggcatct acaccggcgg cctgtggctg
     3541 gccgagcgcc tgcacgccga gctgggcatc gcccagccgc tgggcgcgat cgacgtctcc
     3601 ttctaccgcg acgactacgc cagcaagggc ctgcacgcca gcccgaagaa gaccgagatc
     3661 cccttcgacg tcgacggcgc gcacgtgatc ctggtcgacg acgtgctcta caccggccgc
     3721 accacccgcg ccgcgctcaa cgagctgttc gacttcggcc gcccggcccg ggtcgagctc
     3781 gccgtgctgg tcgaccgcgg cgggcgcgaa ctgccggtcg ccgcgcgcta ctgcgcccac
     3841 accctgtccg tgccgctcgc gcgcgagcag aacctcgagc tgcagcgcga cgaggccggc
     3901 cacttgggcc tcgagctgac acggcgcgcc tgagccggag ccgccctccc cttccccctg
     3961 cccaccgctg attccgaggc acgcccgatg cgaaaccccc agctcaacca gcacggcgaa
     4021 ctgcagcacc tgctcacgct cgacggtctg ccgcgcaaca tcctgaacgc catcctcgac
     4081 accgccgcgc ccttcaccga ggtggccgag cgcgaggtca agaagctgcc gctcttgcgc
     4141 ggcaagagcg tgttcaacct gttcttcgag aactccacac gcacccgcac caccttcgag
     4201 atcgccgcca agcgcctgtc ggccgacgtg gtgaacctca acatcaacac cagctcggcc
     4261 gccaagggcg agagcctgct cgacaccgtc gacaacctgt gcgcgatgca ggccgacatg
     4321 ttcgtcgtgc gccacgccgc cagcggtgcg ccgatgctga tcgcccagca cctgcagaac
     4381 accgggcgcg accacatcca cgtggtcaac gccggcgacg gccgccatgc gcacccgacg
     4441 cagggcctgc tcgacatgta caccatccgc cacttcaaga aggacttcac caacctgacc
     4501 gtggcgatcg tcggcgacgt gctgcactcg cgtgtggcgc gcagccagat cgccgcgctg
     4561 accacgctcg gcgtgcccga agtacgcgtg atcggccccc ggaccctgct gcccaccgag
     4621 gtcgagaagc ttggcgtgcg cgtgttccac gacatgcgcg aggggctgaa aggggtggat
     4681 gtggtgatga tgctgcgcct gcagaacgag cgcatgaacg gcgcgctgct gcccacgccg
     4741 caggagtact acaagatctg gggcctcacc gccgagaagc tcgcgctcgc caggcccgac
     4801 gcgatcgtga tgcaccccgg cccgatgaac cgcggcgtgg agatcgactc cgcggtggcc
     4861 gatggcgcgc aggcggtcat cctgccccag gtcaccttcg gcatcgccgt gcgcatggcg
     4921 gtgatgagca tgctcggcag ccgctgaagc cgcggacagc acaggaacaa ggacccacgc
     4981 atgaacatcc tgatttccaa cggccgcgtc gtcgatccgg ccaaccgcat cgacgcggtg
     5041 cacaacgtct atgtctccgg cggcaagatc gtcgccctcg gccaggcgcc ggacggcttc
     5101 gtcgccgagc gcacgatcga cgccggcggt ctcgtggtcg cccccggctt catcgacctc
     5161 gccgcgcgcc tgcgcgaacc cggctacgag taccgcgcca cgctcgaatc cgaaatggag
     5221 gcggcgatgg ccggcggcgt caccagcctg gcgatcccgc ccgacaccga ccccgtgctc
     5281 gacgagcccg gcctggtcga gatgctgacc tatcgcgcca agaagctgaa ccgcgcccac
     5341 atctacccgg tcggcgcact caccatcggc cttcagggcg agcgcctgtc cgagatggcc
     5401 gaactggtcg aggccggctg cgtcgccttc tcgcaggcca acgtgccgct ggtcgacaac
     5461 accgtgctga tgcgcgcgct gcaatacgcc gccaccttcg gcttccgcgt ctggctgcag
     5521 ccgctggcgc ccttcctgtc gcaggtgggc cacgcccacg acggcgaggt ggcgacgcgg
     5581 ctgggcctgt cgggcatccc ggtcgccgcc gaaaccgtgg cgctctacac ctacctcgag
     5641 ctcgcgcgca tcaccggcgc ccgcctgcac atcacccgcc tgtcctcggc cgccggcctc
     5701 gcgctcatcg accaggcgcg cgcagaaggc atggacgtga cctgcgacgt gtcgatcaac
     5761 catgtgcacc tgtgcgacat ggacatcggc tacttcaacc ccaactgcca cctcgtcccg
     5821 ccgctgcgca gccagcgcga ccgcgaggca ctcgcccggg gcctcgccga gggccgcatc
     5881 gacgcgctgt gctccgacca caccccggtg gacgacgacg ccaagcagac gccgttctcc
     5941 gaatccgaac ccggcgccac cggcctcgag ctgctgctgc cgctgacgct gaagtgggcc
     6001 gaccgcgccg ggctggcgct gctggacggg ctggcccgca tcacctcgga cgcggcgaag
     6061 atcgtcggca tcaccaaggc cggccacctc tcggtgggcg cgcgcgccga cgtgtgcgtg
     6121 ttcgaccccg cgacccacgt caccatcacc cgcgagggcc tccggagcca gggcaagaac
     6181 acgcccttcc tcggcatgga gctgccgggc aaggtgcgct acacgctggt cgaggggcag
     6241 gtgatgttcg agggctgagc ccggtcaggc aaacgcccgc cggcccggct cgcaaaaagg
     6301 ggcagccgct accggctgcc cctttttgcg cttgaagccg gtgcggaaat cagcccccga
     6361 acagcagcgg cgcgaaaccg ttgcgcgcgc cggcgagcac catcagcacg atctgcagca
     6421 ccagcagcag ca
//
LOCUS       DAOQNN010000168         6382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6835_length_6382_cov_10.219851, whole genome shotgun sequence.
ACCESSION   DAOQNN010000168 DAOQNN010000000
VERSION     DAOQNN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6835_length_6382_cov_10.219851"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..1170)
                     /locus_tag="PKV97_09915"
     CDS             complement(283..1170)
                     /locus_tag="PKV97_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06232.1"
                     /translation="MTTTRRIAPLALACALLAAPAASQAATWSDTFVGYRYGSDFREP
                     TNTKDVEKHVLQFTHASGYSVGQNFLNLDIFQSDKADPANGGGSGATEFYLTYRHQVH
                     LGKAFDRSFAFGPVKEVAVTAGFDLNTKNTAFSPRKRLVVLGPTLKFDVPGFLDVSLL
                     VGKEWNRCGLDPVAPSSFDPCPQTEVSFDPQWIVSAAWGIPFSAGAVPLKFQGFININ
                     GEKGKDYAGIKTHTETLMRTSLMVDVGQMAWGKKNTFLMGVGYEYWRNKFGNHAFANG
                     AEKPGIDTDAPTFQMEWHF"
     gene            1397..2143
                     /locus_tag="PKV97_09920"
     CDS             1397..2143
                     /locus_tag="PKV97_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNB06233.1"
                     /translation="MSEDTGNEDLQRDELAEEHEEDGAQRGYTFEDMRLAVGDRLQIE
                     CAASTGVRRAFVRVVGYVDGISLLVTTPVAGGRRVELVENDPVIVRVFSRQSAFAFRA
                     SVLRACRLPLDYVHLSFPSVIQGSVIRKSTRVRTQFPVQVNTQAGGAAGEGTMLNLSA
                     TGALIQTHASLGEPGAIVRLRFDVDLHGLQTELDIEAEIRNVQQAADAEAEGIEYHYG
                     VDFHELGAEDRMKIKSLVYQTIIEQPRRII"
     gene            complement(2164..2367)
                     /locus_tag="PKV97_09925"
     CDS             complement(2164..2367)
                     /locus_tag="PKV97_09925"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNB06234.1"
                     /translation="MLREQASTLNFDVWVPVGRLGECLARMRAKLERGWLTYSRTPAE
                     IAPMRTLKAALDPRRILNPGKML"
     gene            complement(2435..3484)
                     /gene="murB"
                     /locus_tag="PKV97_09930"
     CDS             complement(2435..3484)
                     /gene="murB"
                     /locus_tag="PKV97_09930"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNB06235.1"
                     /translation="MATGDSAGDVAEAAVVLDADLSALNTFGLRAHAARLLRVRSVAA
                     LRAAIAAPGWSDTARLVLGGGSNLVLGGDFAGTVLRVEIPGRRLLRAEADAWIVEGGG
                     GESWHDFVRWTLAQGWPGLENLSLIPGTVGAAPIQNIGAYGVELVERFESLDAVELDT
                     GELRRFDAAACAFGYRDSVFKRTPGRWLVVAVRFRLPRPWRALTRYADVARELEARAL
                     AQPSALEVSDAVIAIRRRKLPDPAVLGNAGSFFKNPVVDAARCARLLAAHPALPHYPQ
                     ADGSEKLAAGWLIEQAGWKGRDLGPVGCYERQALVLVNRGGATGADVRRIAQAIIADV
                     EARFGVRLEPEPVFI"
     gene            complement(3486..3965)
                     /locus_tag="PKV97_09935"
     CDS             complement(3486..3965)
                     /locus_tag="PKV97_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HNB06236.1"
                     /translation="MSTKPKLRSFPDRLRQIAMFEIGGLVLITPPFAWASGVPIRDSI
                     GLLALLALMAAIWNGAYNTAFDWIEGRLTGRTADRRPFRLRAVHALGFESGLLVMTLP
                     VIVWWTGMGWVEALIADIGLAITYTIYAFLFNLGYDRLFPIAPAGGEADAAGLAAER"
     gene            complement(4332..5273)
                     /locus_tag="PKV97_09940"
     CDS             complement(4332..5273)
                     /locus_tag="PKV97_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HNB06237.1"
                     /translation="MVDAVPFFFALGAVASLARSGLRLPAAVFETLSIYLLLAIGLKG
                     GVEIARSESATLWVDALLAIGLGALIPLLVFPLFRLRFSRPDAASLSAHYGSVSIVTF
                     AVGAAVLAGRGIEVEGHLALLAALMEAPALIVATLIARWGVKSADGSSTAGALVHEVF
                     ANKSVVLLGGGILIGWIAGPQGLEPLSPLFVDLFKGALCLFLLEMGLIAADRLPDLKK
                     AGLFLVGCALALPPLLALAGWGVAQAMGLGVGGTVLMMTLAGSASYIAAPTAMRIAVP
                     EANPALGVAAALAVTFPFNLLLGIPLYLWWAQAMAGA"
     gene            5463..>6382
                     /locus_tag="PKV97_09945"
     CDS             5463..>6382
                     /locus_tag="PKV97_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB06238.1"
                     /translation="MRLTFHQLRLLLAVSREGSVSRAAQRLHLTQPTLSAQLRQLAEQ
                     VGLPLFERVGRRLHLTAAGRVVLDTANRVEQELERLDETLAELRGDVVGRLRLAVVST
                     AETFIPRLLGDFRRERPAVEVSLAVLNRDAVIRRLADNLDDLYIMSRPPQAPPVVATP
                     FLSNPLVVVASTAHPLAGCRRKLEIEAIAAEEFVLREPGSGTRQAAEQFFAAHGLALR
                     PRLELGSNEAVKQAVAGGLGLSLLSAHALAHAVDEGIAVLRVEGTPLPTQWQVVYPAG
                     KRLTPLAGAFLEFLRERAAELEHSAQARLDA"
BASE COUNT         1004 a         2234 c         2271 g          873 t
ORIGIN      
        1 agctgcttgg ggttatgaca tgaaggtgcg attgccctgg cgcagggctc gccgcatcgc
       61 ggcagcccgc cacctcggat tcgggttgcg tgcggcgcct tgcgcgccgc ggtggctgtc
      121 acgcggcacc ctgcccgacc gtcgatcgcg actcgcgtcg ctcccaccgc gcggcgcggg
      181 aaatcgatcg cgactcgcgt cgctcccacc gcgcggcacc ggaagcgcgg cgcgggaggc
      241 gcggcggttt tgtaggagcg acgcgagtcg cgatcgatac gctcagaaat gccactccat
      301 ctggaaggtc ggcgcgtcgg tgtcgatgcc gggcttctct gcgccgttcg cgaaagcgtg
      361 gttgccgaac ttgttgcgcc agtattcgta gccgacgccc atcaggaagg tgttcttctt
      421 gccccaggcc atctggccga cgtcgaccat cagcgaggtg cgcatcaggg tctcggtgtg
      481 ggtcttgatg ccggcgtagt ccttgccctt ctcgccgttg atgttgatga agccctggaa
      541 cttcagcggc acggcgccgg cgctgaaggg aatgccccag gccgcgctca cgatccattg
      601 cgggtcgaag ctcacctcgg tctgcgggca gggatcgaag gacgacggcg cgaccgggtc
      661 gagaccgcag cggttccact ccttgcccac gagcaggctg acgtcgagga aacccggcac
      721 atcgaacttc agcgtgggcc ccagcaccac gaggcgcttg cgcggcgaga acgcggtgtt
      781 cttggtgttg agatcgaagc ctgcggtgac cgcgacctcc ttgaccgggc cgaaggcgaa
      841 gctgcggtcg aacgccttgc cgagatgcac ctggtggcgg taagtgaggt agaactcggt
      901 cgccccgctg ccgccgccgt tggccggatc ggccttgtcc gactggaaga tgtccaggtt
      961 gaggaagttc tgcccgaccg aatagccact ggcgtgggtg aactgcagca cgtgcttctc
     1021 gacgtccttc gtgttggtgg gctcgcggaa gtcgctgccg taacggtagc cgacgaaggt
     1081 gtcgctccag gtcgcggcct ggctcgccgc gggggcggcg aggagggcgc aggcgagggc
     1141 gagtggggcg atgcggcggg tcgttgtcat tttgtgtctc cgttcctgtc cactgattgg
     1201 cttctgcggc gcagcgtcgt gcgcacggcc gccaaactag caggccccgc accgggaccg
     1261 gatccaccca tcgtgatacg acacatcagg aaaagcggat attcgcgagg ccgggcgtgc
     1321 aatccgccct tgcgggcgac gggcggagca gcttcaattg gccatcgggg caatcccgga
     1381 acggccgggc gggacgatga gcgaagacac gggcaacgag gatttgcagc gcgacgagct
     1441 tgcggaggaa cacgaagagg acggggcgca acgcggctac accttcgagg acatgcggct
     1501 ggcggtcggc gaccgcctgc agatcgaatg cgccgcgagc accggcgtgc ggcgcgcatt
     1561 cgtgcgtgtg gtcggctatg tggacgggat cagcctgctg gtgaccacgc cggtggcggg
     1621 cgggcggcgc gtggaactgg tcgagaacga tccggtgatc gtgcgggtgt tctcgcgcca
     1681 gagtgccttc gccttccgcg cctcggtgct gcgcgcatgc aggttgcctc tcgactatgt
     1741 acacctgagc tttccgagcg tgatccaggg cagcgtgatc cgcaagtcga cgcgggtgcg
     1801 cacgcagttt ccggtacagg tgaacaccca ggccggcggc gcggcgggcg agggcacgat
     1861 gctgaacctg agcgcgaccg gcgcgttgat ccaaacccat gcctccctcg gcgaaccggg
     1921 cgcgatcgtc cgtctgcgct tcgacgtgga ccttcacggc ctgcagaccg agctcgacat
     1981 cgaggccgag atccgcaacg tgcagcaggc agccgacgcc gaggccgaag gcatcgagta
     2041 ccactacgga gtggacttcc acgaactcgg cgcggaagac cgcatgaaga tcaagagcct
     2101 ggtctatcag accatcatcg agcagccgcg ccgcatcatc tagggccgcg gcggcccgcg
     2161 gccctacagc atcttgcccg ggttgaggat gcggcgcggg tcgagtgccg ccttgagcgt
     2221 gcgcatcggc gcgatctccg ccggggtgcg cgaatacgtc agccagccgc gctccagctt
     2281 ggcgcgcatg cgcgccaagc actcgccgag ccggcccacc ggcacccaga cgtcgaagtt
     2341 cagcgtcgac gcctgctcgc gcagcacctc ggcgatgccg tcgcggatcg cgcggttgcc
     2401 gccggcgttg cccgggagtt gcggcgcccg tggttcagat gaagaccggc tcgggctcca
     2461 ggcgcacgcc gaagcgcgcc tcgacgtcgg cgatgatcgc ctgcgcgatg cggcgcacgt
     2521 cggcaccggt ggcaccgccg cggttgacca gcaccagggc ctggcgctcg taacagccca
     2581 ccgggccgag gtcgcgcccc ttccagccgg cctgctcgat cagccagccg gcggcgagct
     2641 tctcgctgcc gtcggcctgc ggatagtgcg gcagcgcggg gtgcgcggcg agcaagcgtg
     2701 cgcagcgcgc ggcgtcgacg acgggattct tgaagaagct gccggcgttg ccgagtaccg
     2761 ccgggtcggg cagcttgcgg cggcggatcg cgatcaccgc atccgagacc tcgagcgcgg
     2821 agggctgcgc gagcgcgcgt gcctcgagct cgcgcgcgac atcggcatag cgcgtgagcg
     2881 cgcgccacgg ccgtggcagg cggaagcgca ccgcgacgac cagccacctt ccgggagtgc
     2941 gcttgaacac gctgtcgcgg tagccgaagg cgcacgccgc ggcatcgaag cggcgcagct
     3001 cgcccgtgtc gagctcgacc gcgtcgagcg actcgaagcg ctcgaccagt tccaccccgt
     3061 aggcgccgat gttctggatc ggcgcggcac cgaccgtgcc gggaataagc gacaggttct
     3121 cgagcccggg ccagccctgc gccagcgtcc agcgcacgaa atcgtgccag ctctcgccgc
     3181 cgccgccctc gacgatccag gcatcggcct cggcgcgcaa caggcggcgg cccggaatct
     3241 ccacccgcag caccgtgccg gcgaaatcgc cgccgagcac caggttgctg ccgccaccga
     3301 gcaccaggcg cgccgtgtcg ctccagcccg gcgcggcgat cgccgcccga agcgccgcaa
     3361 ccgaacgcac gcgcagcagg cgggccgcgt gcgcacgcag gccgaaggtg ttgagcgcgg
     3421 agaggtcggc gtcgaggacg accgccgcct ccgccacatc acccgcgctg tcgccggtcg
     3481 ccatctcagc gctccgccgc cagcccggcc gcgtccgcct cgccgccggc gggcgcgatc
     3541 gggaacaggc ggtcgtagcc caggttgaac aggaaggcgt agatcgtgta ggtgatcgcc
     3601 aggccgatgt cggcgatcag ggcctcgacc cagcccatcc cggtccacca cacgatcacc
     3661 ggcagcgtca tcaccagcag gccggactcg aagccgagcg catgcacggc acgcagccgg
     3721 aacgggcgac ggtcggcggt gcgcccggta aggcggccct cgatccagtc gaaggcggtg
     3781 ttgtaggcgc cgttccagat cgccgccatc agcgccagca gcgcgagcag gccgatggag
     3841 tcgcggatcg gcaccccgct cgcccaggcg aagggcgggg tgatcaggac caggccgccg
     3901 atctcgaaca tcgcgatctg gcgcaggcgg tcggggaagc tacgaagctt gggttttgta
     3961 gacacggaag ggaactcggg cagcggcggg cgggtcgggc ccacgccccg tcctgcgggt
     4021 cgtcccgcgg gaaaccgacg gtcgcagcgc aaggcgcgca gggcgggcgg ggtcgtcccc
     4081 gcgtcggacc ggatcgggtg tcgaagacac ccgagcggac ttttagcaag tcgcacgcgg
     4141 attggcaagc cggacgaggc gggcgcgtcc gcccgaacgc cgccggcctc ggcgcgcgat
     4201 ggccgccgcg gccggcgacc tcgaagctgc ggaccccgag gcggaaggcc gtacggcctc
     4261 ccgcagcgaa gcggagtccg tgtccgtgac ggaggccgcc ttgccgccgc ctccccaccc
     4321 cggcgccctc ctcaggcccc ggccatcgcc tgcgcccacc acaggtacag cgggatcccg
     4381 agcagcaggt tgaacgggaa ggtcaccgcc aacgccgccg ccacccccag cgccgggttg
     4441 gcctcgggca ccgcgatccg catcgcggtc ggcgcggcga tgtaggaggc gctgcccgcc
     4501 agcgtcatca tcagcaccgt gccgcccacg cccaggccca tcgcctgcgc cacgccccag
     4561 ccggccagcg ccagcagcgg cggcagggcg agcgcgcagc cgacgaggaa cagccccgcc
     4621 ttcttcaggt cgggcaggcg gtcggcggcg atcagcccca tctcgagcag gaacaggcac
     4681 agcgcgccct tgaacagatc gacgaagagc ggcgacagcg gctccaggcc ctgcggcccg
     4741 gcgatccagc cgatcaggat gccgccgccg agcagcacca cgctcttgtt ggcgaagacc
     4801 tcgtgcacca gcgcgccggc ggtcgagctg ccatcggccg acttcacccc ccagcgcgcg
     4861 atcagcgtgg cgacgatcag cgccggcgcc tccatcaggg cggcgagcag ggccaggtgg
     4921 ccttccacct cgatgccgcg tccggccagc accgccgcgc ccaccgcgaa ggtcacgatg
     4981 ctgaccgagc cgtagtgcgc cgacagcgaa gctgcgtccg ggcgcgagaa gcgcaggcgg
     5041 aagagcggga agaccagcag cgggatcagc gcgccgaggc cgatcgccag caacgcatcg
     5101 acccacagcg tggcgctctc cgagcgcgcg atctccaccc cgcccttgag accgatcgcc
     5161 agcagcaggt agatcgacag ggtctcgaag acggcggcgg gcaggcgcag gccggagcgg
     5221 gcaagcgagg ccaccgcgcc gagggcgaag aagaagggaa cggcatcgac catgtgttgt
     5281 tttcctcgat gaacggagcg cgcgggcgcg gaaccggggg agcaccgcgc cggttctccg
     5341 ggggcgcggc tgcgtcagtg cgaggcgaat tttgccgcgg cgcacacatc gagaaaaatg
     5401 atttattctt caacttacca tagatttttt tctatcaatc gaatgacgct cgtccatcgc
     5461 cgatgcgcct gaccttccac cagctccgcc tgctgctcgc ggtgtcgcgc gagggcagcg
     5521 tgtcgcgcgc cgcccagcgc ctgcacctga cccagcccac gctgtcggcg cagctgcgcc
     5581 agctcgccga gcaggtcggc ctgccactgt tcgagcgcgt cggccgccgc ctgcacctga
     5641 ccgcggccgg gcgcgtggtg ctcgacaccg cgaaccgggt cgagcaggag ctggagcgcc
     5701 tcgacgagac cctcgccgag ctgcgcggcg atgtggtggg gcggctgcgg ctggcggtgg
     5761 tcagcaccgc ggagaccttc atcccgcgcc tgctcggcga tttccgccgc gagcgcccgg
     5821 cggtggaggt gtcgctggcg gtgctcaacc gcgacgcggt gatccgacgc ctggccgaca
     5881 acctcgacga cctctacatc atgagccggc cgccgcaggc gccgccggtg gtggccacgc
     5941 ccttcctgtc gaatccgctc gtggtggtgg cctccaccgc gcatccgctc gcgggatgcc
     6001 gcagaaagct ggagatcgag gcgatcgccg ccgaggagtt cgtgctgcgc gagcccggct
     6061 ccggcacccg ccaggcggcc gagcagttct tcgccgccca cggcctcgcc ctgcgcccgc
     6121 gcctggaact cggcagcaac gaagcggtga agcaggcggt ggcgggcggg ctggggctgt
     6181 cgctgctgtc cgcccacgcg ctcgcacatg cggtggacga aggcatcgcg gtgctgcgcg
     6241 tggaaggcac cccgctcccg acccaatggc aggtggtgta tccggccggc aagcgcctca
     6301 ctccgctcgc cggcgccttc ctcgagttcc tgcgcgaacg tgccgccgag ctcgagcaca
     6361 gcgcacaggc gcgcctggac gc
//
LOCUS       DAOQNN010000169         6351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6904_length_6351_cov_10.752859, whole genome shotgun sequence.
ACCESSION   DAOQNN010000169 DAOQNN010000000
VERSION     DAOQNN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6904_length_6351_cov_10.752859"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PKV97_09950"
     CDS             <1..466
                     /locus_tag="PKV97_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNB06239.1"
                     /translation="VDGAALVPLGRAASAASGVACGEIVFDEARARARHAAGAKLILV
                     RQDAETRDLAALELAEGLLTCRGARTAHAAVVARQLGKVCLVGCEALRLDEAGRTLEL
                     GGHILREGELLTLDGNEGAIHAGAAAVVEVVPQALLARLDALHGREVAALQS"
     gene            493..741
                     /locus_tag="PKV97_09955"
     CDS             493..741
                     /locus_tag="PKV97_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06240.1"
                     /translation="MSFRSSYGHKCAIELYRDATERQAKLAGVTIPTFMCRRCGKRQS
                     VSGRRQVVRGSSRYGYHCASCSTARPAGAGCESRPSQV"
     gene            complement(764..2476)
                     /gene="leuA"
                     /locus_tag="PKV97_09960"
     CDS             complement(764..2476)
                     /gene="leuA"
                     /locus_tag="PKV97_09960"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNB06241.1"
                     /translation="MMLQNPAAKYRPFAPIALADRQWPNKLIERAPIWMSTDLRDGNQ
                     ALFEPMNVERKMRMFRTVCEIGFKEIEVGFPSASQTDFDFVRALIEGGHIPADVTIQV
                     LTQAREDLIRRTMESVRGAPRAIIHVYNATSPTFRELVFGMEKPQVLALAVSAVRLIK
                     QIGAEMEKECGTEIRLEYSPETFSATELDFAREVCDAVTAEWGATPENKVILNLPATV
                     EVATPNVYADQVEWMHRNLARRDSVVLSLHPHNDRGSGVAAAELGLMAGADRVEGCLF
                     GNGERTGNVDIVTLALNMYTQGVSPGLDFSDINAVARTVEYCNQLPIHPRHPYVGDLV
                     FTAFSGSHQDAIKKGFAAQKAGQPWNVPYLPIDPADVGRSYDSVIRVNSQSGKGGIAY
                     LLETEYGIVMPRRLQVEFSREVQQVTDASGGEMSAAAIWDLFRSTYLDNVEPVRYVEH
                     HLFEHGAAQGIRLVVEFGGTRHLLVGEGNGPIDAAIHALRGAGIDVQVRSFEERSVAP
                     SEDGGNAQACAFLELTEAGGQGGDRYGVGIDANIVTASIRAILSGVNRLQPDCARAPA
                     ARAA"
     gene            2632..3117
                     /locus_tag="PKV97_09965"
     CDS             2632..3117
                     /locus_tag="PKV97_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNB06242.1"
                     /translation="MERPIVQLDRYDRQILELLQQDGRISNQELADRIALSPSACLRR
                     LRALEESGLIRGYRALLDARKLGLSLMALIHISMDRHTPERFSHFEAEVEKIPQVLEC
                     LLITGQDADYQLKVVVTDMDGYQALLLERITRIPGVTGVHSSFVLRRVIDKTALPLPG
                     A"
     gene            3272..4471
                     /gene="hmpA"
                     /locus_tag="PKV97_09970"
     CDS             3272..4471
                     /gene="hmpA"
                     /locus_tag="PKV97_09970"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HNB06243.1"
                     /translation="MDARVRELVKATIPVLKEHGVALTTYFYQRMFRHNPELKNIFNQ
                     SHNETGKQPVALAMAVLAYAENIDDPSVLAPVITLIANKHASVGIRAEHYPIVGKHLL
                     ASIREVLGDAASDELIAAWGVAYGALADVFIEAESKLYNEACNSDGGWSGWRSFRIID
                     KVRESAEITSFHLVPCDGGPLPSYRAGQYTTVRAFVPELGVTQLRQYTLSIAPGAMHF
                     RISVKREPAGATTPAGRVSNRLHDFYKVGDMLELAPPFGEFFLDETHDGPVVLISGGV
                     GITPMISMLDRLVAAAPSRPVQFVHACRNAAVFAFGAHLAKVAERNPQVRLHLFHDEG
                     AVSAPVQAGRVDLRALAGEVLAEGADYYLCGPVGFLEAQIATLHELGVESARIHAEVF
                     DTGGIAT"
     assembly_gap    4519..4618
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4619..5427)
                     /locus_tag="PKV97_09975"
     CDS             complement(<4619..5427)
                     /locus_tag="PKV97_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNB06244.1"
                     /translation="MTRNTRIARIRRSLAGIATVAIGLFAAQAIAQQPASIVVASTTS
                     TEQSGLFGHILPQFKQATGIDVKVVALGTGQALDVGRRGDADVVFVHDADAERKFVDE
                     GHGIEHRKVMYNDFVLIGPKDDPAAAKGKDITGALQKLAAANGNFVSRGDKSGTHAAE
                     LRYWKAAGVDNKGSGYKECGCGMGPALNMAASSGAYVLADRGTWLSFKNRADLAVLVE
                     GDTRLFNQYGVMVVNPERHPHVKQAEARKFVEWVVSPAGQEAIAAYKIEGE"
     gene            5621..>6351
                     /locus_tag="PKV97_09980"
     CDS             5621..>6351
                     /locus_tag="PKV97_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HNB06245.1"
                     /translation="MKSRVIERWVEAHLAGRRISANSLIISVYGDLIAVHGGVVWLAD
                     LIRLMAPFGLNERVVRTSVYRLVQDDWLESGQVGRRSYYRLAASGMRRFAPAWRRIFE
                     PEGEGWSGTWQVVMLPSALEARVADNLRRELSWAGYGALNAGVLLRPTDDASTLRNIL
                     DGCGLRDRVVPLTARGLDGVTGAATDELVRECWDLQAIAATYDAFIEHFRPLLRAFGT
                     GEVEPQQAFLVQTLLMHEFRRVLLH"
BASE COUNT          901 a         2041 c         2274 g         1035 t
ORIGIN      
        1 cgtcgacggt gccgcgctcg tgccgctcgg ccgcgccgcg agcgcggcga gcggggtggc
       61 ctgtggcgag atcgtcttcg acgaggcgcg cgcccgtgcc cgccacgccg cgggggcgaa
      121 gctgatcctg gtgcgccagg acgccgagac gcgcgatctc gccgcgctcg aactcgccga
      181 gggcctgctc acctgccgtg gcgcacgcac tgcgcacgcc gcggtggtcg cgcgccagct
      241 cggcaaggtc tgcctggtcg gctgcgaggc cttgcgtctc gacgaggcgg ggcgcacgct
      301 cgaactcggc ggtcacatcc tgcgcgaggg cgagctgctg actctggacg gcaacgaggg
      361 cgcgatccat gcaggcgccg cggcggttgt cgaagtcgtg ccgcaggcgc tgctcgcgcg
      421 cctcgacgcg ctgcacggac gcgaggtcgc ggcgctgcag tcctgaagat tttccggcgg
      481 gagggttgtt cgatgagctt cagatccagc tacggccaca agtgcgcgat cgaactgtat
      541 cgcgacgcca ccgagcgcca ggccaagctg gccggtgtga ccattcccac cttcatgtgc
      601 cggcgctgcg gcaagcgcca gtcggtgagc ggacgccgcc aggtggtgcg cggcagttcg
      661 cgctacggct atcactgcgc gagctgcagc actgcgcggc cggctggcgc gggttgcgaa
      721 agccggccgt cccaggtgta gcggcagcgg gtgccgtcgc tgctcaggct gcgcgcgccg
      781 cgggtgcgcg ggcgcagtcc ggctgcaggc gattgacccc gctgaggatt gcgcggatcg
      841 aggcggtgac gatgttggca tcgataccca cgccgtagcg atcgccgccc tgtccgcctg
      901 cctcggtgag ctcgaggaag gcgcaggcct gggcgttgcc gccgtcctcg ctgggcgcca
      961 ccgagcgctc ctcgaagctg cgcacctgca cgtcgatgcc ggcgccgcgc aaggcgtgga
     1021 tggcggcgtc gatcgggccg ttgccctcgc cgaccaacag gtggcgggtg ccgccgaact
     1081 cgaccacgag gcggatgccc tgcgccgcgc catgctcgaa gaggtggtgc tcgacgtagc
     1141 gcaccggctc gacgttgtcg aggtaggtgc tgcggaacag atcccagatc gccgccgccg
     1201 acatctcacc gccgctggcg tcggtgacct gctgcacctc gcgcgagaac tccacctgca
     1261 ggcggcgcgg catcacgatg ccgtattcgg tctcgagcag gtaggcgatc ccgcccttgc
     1321 cggactggct attgacccgg atcaccgagt cgtagctgcg gccgacgtcg gcggggtcga
     1381 tcgggaggta gggcacgttc cacggctggc cagccttttg agcggcgaat cccttcttga
     1441 tggcgtcctg gtgggagccg gagaaggccg tgaacacgag atcccccaca taggggtggc
     1501 gtgggtggat gggcagctgg ttgcagtact cgacggtgcg cgcgaccgcg ttgatgtcgg
     1561 agaagtcgag cccgggcgac acgccctggg tgtacatgtt cagggcgagg gtgacgatgt
     1621 cgacgttgcc ggtgcgctcg ccgttgccga agaggcagcc ctcgacgcgg tcggcgccgg
     1681 ccatcaggcc gagctcggcg gcggcgacgc cgctgccgcg gtcgttgtgc gggtgcaacg
     1741 aaagcaccac gctgtcgcgg cgggcgaggt tgcggtgcat ccactcgacc tggtcggcat
     1801 agacgttggg cgtggcgacc tcgaccgtgg ccgggaggtt gaggatgacc ttgttctcgg
     1861 gcgtggcgcc ccactcggcg gtgacggcgt cgcagacctc ccgggcgaag tcgagttcgg
     1921 tggcggagaa ggtctccggg ctgtattcga ggcggatctc ggtgccgcac tccttctcca
     1981 tttccgcgcc gatctgcttg atcagccgta cggcggagac cgcgagcgcg agtacctgcg
     2041 gcttctccat gccgaacacg agctcgcgga aggtgggcga ggtggcgttg tagacgtgga
     2101 tgatcgcgcg cggcgcgccg cgtaccgact ccatggtgcg gcggatgagg tcctcgcgcg
     2161 cctgggtgag cacctggatg gtcacgtcgg ccgggatgtg gccgccctcg atgagcgcgc
     2221 gcacgaagtc gaagtcggtc tgcgaggccg acgggaagcc gacctcgatc tccttgaagc
     2281 cgatctcgca cacggtgcgg aacatgcgca tcttgcgctc gacgttcatc ggctcgaaca
     2341 gcgcctggtt gccgtcgcgc aggtcggtgc tcatccagat cggggcgcgc tcgatgagct
     2401 tgttcggcca ctggcggtcg gcgagcgcga tcggggcgaa gggacggtac ttggcggcgg
     2461 ggttctgcaa catcatggcg cggtcctcgt gggcatggcg tgattcgatg gacgaaagtc
     2521 tacgcgccgt gcggcggcag acgattgcgt tgttgcggtg cgagcgcgct tatttgtgca
     2581 gaaacatgcg tacttacgaa ctttaatgaa ataatatgcc tttcggaaag aatggagcgc
     2641 ccgatcgtgc aactcgaccg ctacgaccgc cagatcctgg agctgctgca gcaggatggc
     2701 cgcatcagca accaggagct cgccgaccgc atcgcgctgt cgccctcggc ctgcctgcgc
     2761 cggctgcgtg cgctggaaga gtccggtctg atccgcggct accgcgccct gctcgacgcc
     2821 aggaagctcg gcctgtcgct gatggcgctg atccacatct cgatggaccg ccacacgccc
     2881 gagcgcttct cgcacttcga ggccgaggtg gagaagatcc cgcaggtgct cgaatgcctg
     2941 ctgatcaccg gccaggacgc cgattaccag ctcaaggtgg tggtcacgga catggacggc
     3001 taccaggcgc tgctgctcga gcgcatcacc cgtatccccg gcgtcaccgg cgtgcattcg
     3061 agcttcgtgc tgcggcgggt gatcgacaag acggcgctgc cattgccggg ggcctgagcc
     3121 ccgggtgtgc acaaactgga tatatgtcac gttgtgagcg gatagcgggg gcgggtgtct
     3181 gcgtccaaga tcttcgggct gcgtggccct tgcgccgcgc tcgagttcat ccaaatgaag
     3241 tccgccgtgt gcggaccccc aaggagacga catggacgcc cgcgttcgcg aactggtcaa
     3301 ggccacgatc cctgtgctca aggagcacgg cgttgccctg acgacgtatt tctatcagcg
     3361 catgttccgc cacaacccgg aactgaagaa catcttcaac cagtcgcaca acgagaccgg
     3421 caagcagccg gtggcgctgg cgatggcggt gctggcctac gccgagaaca tcgacgaccc
     3481 ttcggtgctg gcgccggtga tcaccctgat cgccaacaag cacgccagcg tgggcatccg
     3541 cgccgagcac tacccgatcg tcggcaagca cctgctggcc tcgatccgcg aggtcctggg
     3601 cgacgccgcc agcgacgagc tgatcgccgc ctggggggtg gcctatggcg cgcttgccga
     3661 tgtcttcatc gaggccgaga gcaagctcta caacgaggcc tgcaacagcg acggcggctg
     3721 gagcggctgg cgcagcttcc gcatcatcga caaggtgcgc gagagcgcgg agatcacgtc
     3781 cttccacctc gtgccctgcg acggcggccc gctgccgtcc taccgcgccg gccagtacac
     3841 gaccgtccgt gcgttcgtgc ccgagctcgg cgtgacacag ctgcgccaat acacgctgtc
     3901 gatcgcgccg ggggccatgc acttccgcat ctcggtgaag cgcgagccgg cgggcgcgac
     3961 cacgccggcc ggtcgggtgt cgaaccgcct gcacgacttc tacaaggtgg gcgacatgct
     4021 cgagctggcg ccgcccttcg gcgagttctt cctcgacgag acccacgacg gcccggtggt
     4081 gctgatcagc ggcggggtcg gcatcacgcc gatgatctcc atgctcgacc gcctggtagc
     4141 ggcggcgccg tcgcgtccgg tgcagttcgt gcatgcctgc cgcaatgccg cggtgttcgc
     4201 cttcggtgcg catctggcga aggtggcgga gcgcaacccg caggtgaggc tgcatctgtt
     4261 ccacgacgaa ggcgcggttt cggcaccggt gcaggcgggc cgtgtcgatc tgcgcgcgct
     4321 ggcgggcgag gtgctggcag agggggccga ctactacctg tgcggcccgg tcggcttcct
     4381 ggaggcgcag atcgcgaccc tgcacgagct gggggtcgag tcggcgcgca tccatgccga
     4441 ggtgttcgac accggcggca tcgcgacctg aggccggggc cggctcgtcc ggcttcgatc
     4501 cggccgcggg gccggggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     4621 ctcgccctcg atcttgtacg cggcgatggc ctcctggccg gcgggcgaaa ccacccattc
     4681 gacgaacttg cgcgcctcgg cctgtttcac gtgcgggtgg cgctcggggt tcaccaccat
     4741 cacgccgtac tggttgaaga ggcgggtgtc gccttcgacc agcaccgcga ggtcggcgcg
     4801 gttcttgaag ctgagccagg tgccgcggtc ggcgagcacg taggcgccgc tcgatgccgc
     4861 catgttgagt gccgggccca tgccgcagcc gcattccttg tatccgctcc ccttgttgtc
     4921 gaccccggcg gccttccagt agcgcagctc ggcggcgtgg gtgccgctct tgtcgccgcg
     4981 cgacacgaag ttgccgttgg cggcggcgag cttctgcagc gcgccggtga tgtccttgcc
     5041 cttggcggcg gccggatcgt ccttcggccc gatcagcacg aagtcgttgt acatgacctt
     5101 gcggtgctcg atgccgtggc cttcatcgac gaacttccgc tctgcgtcgg cgtcgtgcac
     5161 gaacaccacg tcggcgtcgc cgcggcggcc gacgtcgagg gcctggccgg tgccgagcgc
     5221 gaccaccttc acgtcgatgc cggtcgcctg tttgaactgc ggcagaatat ggccgaagag
     5281 accggactgc tcggtggagg tcgtcgacgc gacgacgatc gatgcgggct gctgggcgat
     5341 cgcctgggcg gcgaagaggc cgatcgccac ggtggcgatt ccggcgaggc tgcggcggat
     5401 gcgcgcgatg cgggtgttgc gggtcatgcg gagtctcctc gattgggggt gggctcgcgg
     5461 ggtgggcggt gtcgtggccg tcgcggcggc tgcggcgacg gatgagcctc gggcaatcta
     5521 ggcaggcggg tgggatggat caatatgaag ggatctgcat aggatggagg gccgcagcgg
     5581 tgtggatgcc gccgcgcacc acggacatgg accacagcgc atgaagagcc gcgtgatcga
     5641 acgctgggtc gaggcccacc tcgccggacg gcgcatcagc gccaactcgc tgatcatctc
     5701 ggtgtatggc gacctgatcg cggtgcacgg cggcgtggtg tggctggccg acctgatccg
     5761 gctgatggcg cccttcgggc tcaacgagcg cgtggtgcgc accagcgtct atcggctggt
     5821 gcaggacgac tggctggagt cgggacaggt cggccggcgc agctactacc gcctcgccgc
     5881 atccggcatg cgccgtttcg cgccggcgtg gcggcggatc ttcgagcccg agggggaggg
     5941 ctggagcggg acctggcagg tggtgatgct gccctccgcg ctcgaggcgc gggtggcgga
     6001 caacctgcgc cgggagctgt cttgggcggg ctacggggcg ctcaacgccg gcgtgctgtt
     6061 gcgcccgacc gacgacgcct cgaccctgcg caacatcctc gacggctgcg gcctgcgcga
     6121 ccgcgtggtg ccgctcaccg cgcgcggcct ggacggggtg accggcgcag ccaccgacga
     6181 gctggtgcgc gagtgctggg acctgcaggc gatcgccgcc acctacgacg ccttcatcga
     6241 gcacttccgg ccgctgctgc gcgccttcgg cacgggcgag gtcgagccgc agcaggcctt
     6301 cctggtgcag accctgctga tgcacgagtt ccgccgcgtg ctgctgcacg a
//
LOCUS       DAOQNN010000170         6326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6952_length_6326_cov_8.855207, whole genome shotgun sequence.
ACCESSION   DAOQNN010000170 DAOQNN010000000
VERSION     DAOQNN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6952_length_6326_cov_8.855207"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKV97_09985"
     CDS             complement(<1..228)
                     /locus_tag="PKV97_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNB06246.1"
                     /translation="MRFPRPLVKVIATSLVGLGLAAVAHAQTAWDMPTPYPANNFHTE
                     NIQQFAKEVDEATGGKLKITVHANGSLFKANE"
     gene            410..925
                     /locus_tag="PKV97_09990"
     CDS             410..925
                     /locus_tag="PKV97_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HNB06247.1"
                     /translation="MKEASRIVSSQHLISPKSPELSEMEFGLIIAWHAFARWMMRCMT
                     TAGVKDMTPIDVLVLHHVSHRDSEKRLADICFVLNVEDTHVVSYSLRKLAGLGLVQSV
                     KKGKEAFFSVTERGREICLAYRDVREACLMPGFTGSPEDNAQIGELARLLRTLSGRYD
                     QAARAASTSSL"
     gene            956..1717
                     /gene="pxpB"
                     /locus_tag="PKV97_09995"
     CDS             956..1717
                     /gene="pxpB"
                     /locus_tag="PKV97_09995"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HNB06248.1"
                     /translation="MSFRILDAGDAALTIEFGSIIDPALLAEVNALDAAVLRRKQAGE
                     LPGVIETMPTFRSLTVFFDPLVTDRDAVLAALQPLIAAAEHGSTTDGRHWRLPVCYEG
                     EAAPDLAEVAGAIGIGEDEVVALHSGAEYLVYMIGFLPGFPFMGDLPAPLRLPRRAQP
                     RVRVPAGSVAIATGLTAIYPWESPGGWHLLGRCPVPLFDARRTSPSLLAAGDRVRFVP
                     VSAQECRAIEAGLASAEIDPMQWLESAPTPAENAA"
     gene            1714..2766
                     /locus_tag="PKV97_10000"
     CDS             1714..2766
                     /locus_tag="PKV97_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HNB06249.1"
                     /translation="MSTRTEVEILSPGAFASIQDGGRRGHRRIGVPWAGVLDRRLMRI
                     ANALAGRAEDAAVIECFDGGLHVAASGGAVKLAVAGDAVVEVEGTEGRRQLAPWRSVT
                     LADGEQLRIRKMEGGRIAMVAIVGLEPAAVMGSASTYARAGIGGVDGRALGPGTRLAL
                     SADADPWDSDRVLAQPPAADTGPIRLVPGPQADHFSPTALDALVGGEYRVTTEADRMG
                     IRLEGAQLEHAGAAEIVSDATVPGSIQVPGAGQPIVLLADAQTAGGYPKIATVIGADL
                     GRLAALRPGQSLRFAAVSAAEGACIARAAETETRALIASIRALPPDGIDLMALYTGNL
                     VDGVVHALGTEYRPLY"
     gene            2861..3631
                     /locus_tag="PKV97_10005"
     CDS             2861..3631
                     /locus_tag="PKV97_10005"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HNB06250.1"
                     /translation="MNKTINLNADLGESFGPWTMGEDEALLQVVRSANIACGFHAGDP
                     VVMRHTVRTALATGVSLGAHPAYPDLQGFGRRPMKMAPAELEAMIIYQVGALAGMAAA
                     EGGRVTHVKPHGALNNQACEDAALADAVARAVKAIDPGLILLAPVLSELHAAGARAGL
                     RVAGEVFADRAYTDKGTLVPRGQPGAVIHDHDELVTHVLRMLEAGGVVSQSGKVLPTS
                     MDSICVHGDTLGAVESARHLRTELEARGWTIAPLPQLS"
     gene            complement(3858..4451)
                     /locus_tag="PKV97_10010"
     CDS             complement(3858..4451)
                     /locus_tag="PKV97_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNB06251.1"
                     /translation="MPLTLAAPVHSELIIKKSRFIGCVQPMSDRAGAQKVVAALWAQH
                     PGARHVCWALLAGGQSAAVDDGEPSGTAGRPMLDVLRHQDLEGVLATVVRYFGGVKLG
                     AGGLVRAYTDAVAQALLEAEKVPIVRLATLRCVVPYAMEGLLRRELDAAGASLLAVAH
                     GQAVELCFSLPEDAAAALRARLAEAGQGRIEWIAEVE"
     gene            4631..5626
                     /locus_tag="PKV97_10015"
     CDS             4631..5626
                     /locus_tag="PKV97_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNB06252.1"
                     /translation="MPASAAQDDYDSPWKEAVEHAFPEFIDFYFPDAGRQIDWARGHR
                     FLDKELQQIVRDAALGRRHVDKLASVTTHAGEEDWLCVHIEVQGSMDPDFARRMFVYN
                     YRIYDSYDRPVASLAVLADDDPAWRPDRFGYERLGCRHNLQFPVAKLVDHAADEAALL
                     CNPNPFALVTAAHLYTRRTRRSPIARFDAKRRLVRLLYERDWTRQRILDFFSVLDWMM
                     RLPREFEQRLWQDIENIEGERKVKYVTSVERLAIERGLQKGMEQGLEIGIEKGIEQGR
                     AQGSASVLLRLLNRRFGPLSPDIIRRLSQSTPEQLEIWAERVLEARTIDEVFAGN"
     gene            5729..5998
                     /locus_tag="PKV97_10020"
     CDS             5729..5998
                     /locus_tag="PKV97_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06253.1"
                     /translation="MKADERHYILDMNPADAPIPQDSAPLELMTRAFEGGVGVIQKTN
                     DFPALLAHLQQGMRPFAEACADLNPEAFELPSCQRIVARLFEPID"
     gene            6007..>6326
                     /locus_tag="PKV97_10025"
     CDS             6007..>6326
                     /locus_tag="PKV97_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HNB06254.1"
                     /translation="MARTHPEGWRLLPAEGARGRELATLARLAAALPEDTTIYHGLHW
                     TRAEGRTAVFGEVDFAVVGPGGRVLLVEQQAGFLDETPAGLLRPGRRRAAQLGVELAR
                     NADAL"
BASE COUNT          980 a         2390 c         2052 g          904 t
ORIGIN      
        1 ctcgttggcc ttgaacagcg agccgttggc gtgcacggtg atcttgagct tgccgccggt
       61 ggcctcgtcg acctccttgg cgaactgctg gatgttctcg gtgtggaagt tgttggccgg
      121 gtagggcgtg ggcatgtccc acgcggtctg ggcgtgggcg acggcggcca gacccaggcc
      181 tacgagcgaa gtggcgatca ccttgacgag gggacgcggg aagcgcatgg cgattactcc
      241 tggtagagga gggcgagaag cccggaacgc ggattggcgg gcggcgaccg gtcttcgata
      301 agtcaccgtt attgcacaat agagattttg tgggtatctt gtcaacaaaa cgtacacgtt
      361 ttttttgcaa aacgtccgcg aaaccttgtc ttccaccccg gaggcagtca tgaaagaagc
      421 cagccgcatc gtgtcctccc agcacctgat ctcgccgaaa tcgcccgaac tttccgagat
      481 ggagttcggc ctcatcatcg cctggcatgc tttcgcacgc tggatgatgc gctgcatgac
      541 caccgccggc gtcaaggaca tgacgccgat cgacgtgctg gtgctgcacc acgtgtcgca
      601 ccgcgactcc gagaagcgcc ttgcggacat ctgcttcgtg ctcaacgtcg aggacaccca
      661 cgtcgtctcc tactcgctgc gcaagctcgc cggcctcggc ctggtgcaga gcgtcaagaa
      721 gggcaaggag gcgttcttct ccgtcaccga gcggggccgc gagatctgcc tggcgtaccg
      781 cgacgtacgc gaggcctgcc tgatgcccgg cttcaccggc agccccgagg acaacgccca
      841 gatcggcgaa ctcgcccgcc tgctgcgcac cctgtccggc cgctacgacc aggccgcgcg
      901 cgcggcctcc acttcatcgc tgtaaatccg ctgcacatca ccagggagcc ctcccgtgag
      961 ctttcgcatc ctcgacgccg gcgacgccgc gctcaccatc gaattcggca gcatcatcga
     1021 ccctgccctg ctggccgagg tcaatgcgct cgacgccgcc gtgctgcgcc ggaaacaggc
     1081 gggcgaactg ccaggcgtga tcgagaccat gcccaccttc cgctcgctga ccgtgttctt
     1141 cgacccgctc gtcaccgacc gcgatgcagt gctcgccgcg ctgcagccgc tgatcgccgc
     1201 cgccgagcac ggcagcacca ccgacggccg ccactggcgc ctgccggtgt gctacgaggg
     1261 cgaggccgcc cccgacctcg ccgaggtggc gggcgcgatc ggcatcggcg aggacgaggt
     1321 cgtcgcgctg cacagcggag cggaatacct ggtctacatg atcggcttcc tgcccggctt
     1381 ccccttcatg ggcgacctgc ccgcgccgct gcgcctgcca cgccgcgccc agccgcgcgt
     1441 gcgcgtgccc gccggaagcg tggcgatcgc cactggcctg accgcgatct acccctggga
     1501 gagccccggc ggatggcacc tgctcgggcg ctgcccggtg ccgttgttcg acgcccgccg
     1561 cacctcgccc tcgctgctcg cggccggaga ccgcgtgcgc ttcgtcccgg tgagcgccca
     1621 ggaatgccgc gccatcgagg ccggcctcgc cagcgccgaa atcgacccca tgcaatggct
     1681 ggaaagcgcc ccgacccccg cggagaacgc agcatgagca cccggaccga agtggaaatc
     1741 ctctcccccg gcgccttcgc ctccatccag gacggcggcc gccgcggcca ccgccgcatc
     1801 ggcgtgcctt gggcgggcgt gctcgaccgc cgcctgatgc gcatcgccaa tgcgctcgcc
     1861 ggtcgcgccg aggacgccgc ggtgatcgaa tgcttcgacg gcggcctgca cgtcgccgcg
     1921 agcggcggcg cggtgaagct cgcggtggcc ggcgacgcgg tggtcgaggt cgaaggcacc
     1981 gagggccggc gccagctcgc gccgtggcgc tcggtgacgc tggccgacgg cgagcagctg
     2041 cgcatccgca agatggaggg cggacgcatc gccatggtcg cgatcgtcgg cctcgagccg
     2101 gcggcggtga tgggcagcgc ctcgacctat gcgcgcgccg gcatcggcgg cgtggatggc
     2161 cgtgcgctcg gccctggcac gcgcctggcg ctctccgccg acgccgaccc ctgggacagc
     2221 gaccgcgtgc tcgcccagcc gcccgcggcc gacaccggtc cgatccgcct ggtgcccggt
     2281 ccgcaggccg accacttcag ccccaccgcg ctcgacgccc tggtgggcgg cgagtatcgc
     2341 gtcaccaccg aggccgaccg catgggcatc cgcctcgagg gcgcgcagct ggagcacgcc
     2401 ggcgccgccg agatcgtctc cgacgccacc gtgcccggct ccatccaggt gcccggtgcc
     2461 ggccagccca tcgtgctgct cgccgacgcg cagaccgccg gcggctatcc caagatcgcc
     2521 accgtgatcg gcgccgacct cggccgtctc gccgcgctgc gccccggcca gagcctgcgc
     2581 ttcgccgccg tgagcgccgc cgagggcgcg tgcatcgcgc gcgccgccga gaccgagacc
     2641 cgggcgttga tcgcctcgat ccgcgccctg ccgcccgatg gcatcgacct gatggcgctg
     2701 tacaccggga acctggtcga cggcgtcgtg catgccctcg gcaccgaata ccgaccgctg
     2761 tattgatcgc gcaagcgccg cccgccctgg cgcgacgcaa gtcgcaatcc cggcccccag
     2821 cctgcacgcc ccgatctccc caccccaagg agccccgccc atgaacaaga cgatcaacct
     2881 caacgccgac ctcggcgaat ccttcggccc ctggaccatg ggcgaggacg aggcccttct
     2941 gcaggtggtg cgctcggcca acatcgcctg cggcttccac gccggcgacc cggtggtgat
     3001 gcgccacacc gtgcgcacgg cgctcgcgac gggggtgagc ctcggcgccc atccggccta
     3061 ccccgacctg cagggcttcg gtcgccggcc gatgaagatg gcgccggccg agctcgaggc
     3121 gatgatcatc taccaggtcg gcgcgctcgc cggcatggcc gcggccgaag gtggccgcgt
     3181 cacccacgtc aagccgcacg gcgcactcaa caaccaggcc tgcgaggatg cggcgctcgc
     3241 cgacgcggtc gcgcgcgcgg tcaaggccat cgaccccggg ctgatcctgc tcgccccggt
     3301 gctgtcggaa ctccatgcgg ccggcgcgcg cgccgggctg agggtcgccg gcgaggtctt
     3361 cgccgaccgc gcctacaccg acaagggcac gctggtgccg cgcggccagc ccggcgcggt
     3421 catccacgac cacgacgagc tcgtcaccca cgtgctgcgc atgctggagg ccggcggcgt
     3481 ggtcagccag agcggcaagg tgctgccgac ttcgatggac agcatctgcg tgcacggcga
     3541 caccctgggc gcagtggaga gcgcgcgaca tctgcgcacg gaactcgaag cgcgcggctg
     3601 gaccatcgcc cccctgccac agctctcctg aggccgaccc gccggcggat caggcaggag
     3661 cgacgcaagt cgcgatttcg gcgcttgatc tgcgcatgcg ccggatacgc cggggggagg
     3721 attccggatg attcgatcgc gacttgcgtc gctcctacga ccacacccta cgccccaggc
     3781 gccctgtagg agcgacgcaa gtcgcgatcc tcgcccccgc gcaaacccgg acgctgccgc
     3841 cccttggcgc ccaacgctca ctcgacctcc gcgatccact cgatccggcc ctgccccgcc
     3901 tccgcaaggc gcgcgcgcag ggctgcggcg gcatcctcgg gcaggctgaa gcacagctcc
     3961 accgcctgcc cgtgcgccac cgcgagcagg ctcgccccgg cggcgtcgag ctcgcgccgc
     4021 agcagcccct ccatcgcata cggcaccacg cagcgcagcg tcgccaggcg cacgatcggc
     4081 accttctcgg cctcgagcaa ggcctgcgcc accgcgtccg tgtaggcgcg caccagaccc
     4141 ccggcgccca gcttcacgcc gccgaagtag cgcaccacgg tggcgagcac gccctccagg
     4201 tcctggtggc gcagcacgtc gagcatcggc cgcccggcgg tgccgctcgg ctcgccgtcg
     4261 tccaccgccg ccgactgccc gcccgcaagc agcgcccagc acacatggcg cgcccccgga
     4321 tgctgcgccc acaacgcggc cacgaccttc tgcgcgccgg cgcggtcgct catcggctgc
     4381 acgcagccga tgaagcggct cttcttgatg atcagttcgc tgtggacggg agcggcgagg
     4441 gtcagcggca tgggggggat tctagtccga ttcgcgcggc ggcacggcgg cggccagccg
     4501 gcattgccat ggcgtgcagc ccccgaaaat cgcggatact ccgaaaattc ggggacgcat
     4561 cccaatgccc ccaattccct tttgtcatat ttccaaatag cattccagct ccctcaccca
     4621 ggagctcgca atgcccgcca gcgccgcgca ggacgattac gacagtccgt ggaaggaggc
     4681 cgtcgagcac gccttcccgg agttcatcga cttctacttc cccgatgccg gccgccagat
     4741 cgactgggcg cgcggccacc gcttcctcga caaggagctg cagcagatcg tacgcgacgc
     4801 cgcgctcggc cggcgccatg tcgacaagct cgccagcgtc accacgcacg ccggcgagga
     4861 ggactggctg tgtgtgcaca tcgaggtcca gggaagcatg gaccccgact tcgcacgccg
     4921 gatgttcgtc tataactacc gcatctacga cagctacgac cgtccggtcg ccagcctggc
     4981 cgtgctcgcc gatgacgacc cggcctggcg ccccgaccgc ttcggctacg agcgcctcgg
     5041 ctgccggcac aatctgcaat ttccggtcgc caagctggtc gaccacgcag cggacgaagc
     5101 cgcgctgctg tgcaacccga accccttcgc actcgtcacc gccgcgcacc tctacacccg
     5161 tcgcacccgc cgcagcccga tcgcccgctt cgacgccaag cgccgcctgg tgcgactact
     5221 ctatgaacgc gactggacgc gacaacgcat cctcgacttc ttttcggtgc tcgactggat
     5281 gatgcgacta cccagggagt tcgagcagag actgtggcaa gatattgaaa atattgaagg
     5341 agaacgcaaa gtgaagtacg tcaccagcgt cgaacgactc gcgatcgagc gcgggctgca
     5401 gaagggcatg gagcaagggc tcgaaatcgg tatcgagaag gggatcgagc agggtcgtgc
     5461 ccaaggcagt gcaagcgtcc tcctgcgcct gctgaaccgg cgcttcggcc cgctctcccc
     5521 cgacatcatc cggcgtctct cccagtccac gcccgagcag ctcgagatct gggccgagcg
     5581 cgtgctcgag gcgcggacga tcgacgaggt gttcgcgggg aactgaagca cgggctcacg
     5641 ctggacgggg gtgagcggcg tgcgcaaacc gccgcaaccc cgccggtctc cgatgggtga
     5701 agcgatccgg ccacttcgcc ccgcaaccgt gaaagcggat gaacgccatt acattctcga
     5761 catgaacccc gccgacgcgc ccatcccgca ggactccgcc ccgctcgagc tgatgacccg
     5821 cgccttcgaa ggcggcgtcg gcgtgatcca aaagacgaac gacttccccg ccctgctcgc
     5881 ccatctgcag caggggatgc gtcctttcgc cgaggcctgc gcggatctga accccgaggc
     5941 cttcgagctc ccgtcttgcc agcgcatcgt cgcccgcctg ttcgagccca tcgactgagg
     6001 cccaccatgg cccgcaccca ccccgaaggc tggcgcctgc tccccgcgga gggcgcgcgc
     6061 gggcgcgagc tcgccaccct cgcccgcctc gccgccgcgt tgcccgagga caccacgatc
     6121 taccacggcc tgcactggac gcgcgccgag ggtcgcacgg cggtgttcgg cgaggtcgat
     6181 ttcgccgtgg tcggcccggg cgggcgggtg ctgctggtcg aacagcaggc cggcttcctc
     6241 gacgagacgc ctgccggcct gctgcgcccc ggcaggcgcc gcgcggcgca gctcggcgtc
     6301 gagctcgcgc gcaacgccga cgccct
//
LOCUS       DAOQNN010000171         6305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_6983_length_6305_cov_8.149280, whole genome shotgun sequence.
ACCESSION   DAOQNN010000171 DAOQNN010000000
VERSION     DAOQNN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6983_length_6305_cov_8.149280"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="PKV97_10030"
     CDS             <1..1394
                     /locus_tag="PKV97_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HNB06255.1"
                     /translation="LSVELARNADALRARLRPLLDAAEPVLDVLLHCPDHCVRQPGTA
                     GLDPERIVDAGRRDQLPAIVAALTRPQESDPALDAAHRQALHRFFADLLELVPDVQSH
                     IGQVEDLTTRLSGGLTEWARRIVVEPHRLRVTATAGSGKTQLALAAYTDALAAGRRPL
                     YVCYNRPLADHFARIAPAGGEIATYHQLCDRRVRDAGRKPAFGAPGAFRRMEADFATL
                     VREQPDPAWQFDELIIDEGQDFTEPWRDALLALLKPAGRAWWLEDPLQNLYDRPPVAL
                     PGWVGLSADTNYRTPADVLALLNARLPLPAPVRAGSPVTDSEPEIFAWRDEAGLMDAT
                     KRAITRALGLGFRRDAIVLLTFRGREHSRFTPLDHLGPHRLRAFTGRYDLLGEPEHSQ
                     GELLIDSVYRFKGQSAPCILFTEIDFETTGGRMDELTMRKLFVGATRATMKLILVASE
                     RAAAALAPVAEAG"
     gene            complement(1571..2626)
                     /locus_tag="PKV97_10035"
     CDS             complement(1571..2626)
                     /locus_tag="PKV97_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06256.1"
                     /translation="MKVTASSVELQSSHRFAQSLETRERLEVWRGTRDSGSAAESTQP
                     PVVALSAAGQNALAAESATAAVDAVAEEAVEDDPRLAMLIRMIEFLTGEPVRRFSMRD
                     LQSADAAEPGYETGANPGTSGQAGARRAGFGLEYDFSSRFSETETISFGAAGVVRTAD
                     GAEIRFELGFEMSRSYTESVAVSVRAGDQRLKDPLVLDFGGPAAALSEVRFDFDLDSD
                     GTREKLPLVSGSGFLAFDRNANGRIDDGRELFGPASGDGFSELARLDDDANGWIDEAD
                     AAWSQLRVWRPDAAGKGSVRSLSEAGVGALHLGRVATPFSLKGAAHDTQGVMRASGVY
                     LREDGGVGTLSQVDLKV"
     gene            2792..4261
                     /locus_tag="PKV97_10040"
     CDS             2792..4261
                     /locus_tag="PKV97_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB06257.1"
                     /translation="MSTFYIEMRSIAVHASISLAFFDDIPLGRSRMSAPRAYRLLLAL
                     AAAATVSSSHAAAIASQPFDIGVDMVQVFNDRLGAGRFFAGAGTDRLRVSARVTPSPD
                     TDFYDQSSNGGETAIFVTHPSTPGLDTRLQFVGLQSGGGGGKNDYTTTFNLANPFVQG
                     QLAAWDATPFALRIENPSAPGGETTKWVTAPDYDSSVNLPFLQDVAITGGGLAPTLSW
                     TVPDTTAPITNIRIQLRRIDAEEPGRITAATLVHEAVLPLGSNGYTLDQAFSNGGLPG
                     LPSGLEQGSKYEVAVILESAEPGLIKGRARTFFEFTPLTDDAGDRTIYLPSVDSAGNF
                     NFDIEVRAGETILIDPVVAVGYDYQIGSGDPLFASVTLPNVGDGIFELWLYDLNLADF
                     VFETFLDAGERFDFTGGGVDRFRVLGIEPSAGLDPTDVQAFVTEIGFTGNGRFTGRMT
                     PITTNFTPVPEPSSLALLALAVLALGGLRRSCRAGGSLR"
     gene            4296..4574
                     /locus_tag="PKV97_10045"
     CDS             4296..4574
                     /locus_tag="PKV97_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNB06258.1"
                     /translation="MSNKHRHTLEAIFRDPPAGNLHWREVESLLSHLGAEVESGHGAR
                     FRVVLNRREFYLHHPHHGSDFGKQAVKDLREFLASAGVSPSSYQDGTG"
     gene            complement(4699..6087)
                     /locus_tag="PKV97_10050"
     CDS             complement(4699..6087)
                     /locus_tag="PKV97_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HNB06259.1"
                     /translation="MNDILRVAVVGHTNTGKTSLLRTLARDVGFGEVSDSAGTTRHVE
                     GLRLMADGVPAAELFDTPGMEDAIALLEFIDDLVDPGERVDGPERVARFLARDEAAGR
                     FEQEAKVLRQMLASDAALYVVDARDPVLPKHRDELELLAACARPLLPVLNFIADPVAR
                     ADDWRGALARLGLHAVVSFDTVAPPLDGERELFDTLATLIHSHRGRLQALIEARAREA
                     AARREAARRLVAELLVELAARRDRLADSEDDEAALQRAVERLRADVRSREQACVDALL
                     RLYRFRADDARMAELPLADGRWDDDLFNPETLRQMGVRLGTGAAAGAAAGVGIDLMTG
                     GLTLGAAAALGAIAGGLWQTFGHYGERLAAKLRGHRELTVDDAILRIVALRQRSLLVA
                     LDGRGHAAVKPIELDAGRAGMPMIADPVSVRWRKDALPEAIGRARAHPEWAEADGGHK
                     DEAIAELARSLA"
     gene            complement(6084..>6305)
                     /locus_tag="PKV97_10055"
     CDS             complement(6084..>6305)
                     /locus_tag="PKV97_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06260.1"
                     /translation="RTGRNQNHLAGAELADHAQATRIWLAGVDAAAERAGRLRQWREG
                     LAGIGLGEEAALADAGAACGWLERGDEAR"
BASE COUNT          926 a         2369 c         2116 g          894 t
ORIGIN      
        1 agctcagcgt cgagctcgcg cgcaacgccg acgccctgcg cgcccgcctg cgcccgctgc
       61 tcgacgccgc cgagcccgtg ctcgacgtgc tgctgcactg ccccgaccac tgcgtgcgcc
      121 agcccggcac cgccggactc gaccccgagc gcatcgtcga cgccggccgg cgcgaccagc
      181 tccccgcgat cgtcgccgcg ctgacgcggc cgcaggagag cgacccggcg ctcgacgccg
      241 cgcaccgcca ggccctccac cgcttcttcg ccgacctgct cgagctggtg ccggacgtgc
      301 agtcgcacat cggccaggtc gaagacctca ccacccggct gtcgggcggg ctcaccgagt
      361 gggcgcgccg catcgtggtc gagccgcacc gcctgcgcgt gaccgccacc gccggcagcg
      421 gcaagaccca gctcgcgctc gccgcctaca ccgacgcgct cgccgccggc cggcgcccgc
      481 tctacgtgtg ctacaaccgt ccgctcgccg accacttcgc gcgtatcgcg cctgccggcg
      541 gcgagatcgc cacctaccac cagctctgcg accgccgcgt gcgcgacgcc ggccgcaagc
      601 cggccttcgg cgcgcccggc gccttccgcc gcatggaggc ggacttcgcc accctggtgc
      661 gcgagcagcc cgatcccgcc tggcagttcg acgagctcat catcgacgag ggccaggact
      721 tcaccgagcc ctggcgcgat gccctgctcg cgctgctgaa gccggcgggg cgcgcctggt
      781 ggctggaaga cccgctgcag aacctctacg accgtccgcc ggtggcgctg cccggctggg
      841 tggggctgag cgccgacacc aactaccgca ccccggccga cgtgctcgca ctcctcaacg
      901 cccgcctgcc actgcccgcc ccggtgcgcg ccggcagccc ggtgaccgac agcgaaccgg
      961 agatcttcgc ctggcgcgac gaagccgggc tgatggatgc caccaaacgc gcgatcacgc
     1021 gcgccctcgg cctgggcttc cgccgcgacg cgatcgtgct gctgaccttc cgcggccgcg
     1081 agcattcgcg cttcaccccg ctcgaccacc tcggccccca ccgcctgcgc gccttcaccg
     1141 gccgctacga cctgctcggc gagccggagc actcgcaggg cgaactgctg atcgattcgg
     1201 tgtatcgctt caagggccag tcggcgccct gcatcctgtt taccgagatc gacttcgaga
     1261 cgacgggcgg gcgaatggac gagctcacca tgcgcaagct tttcgtcggc gccacgcggg
     1321 cgacgatgaa gttgatcctg gtggcgtcgg agcgtgcggc ggccgcgctc gcgccggtcg
     1381 cggaagcggg ctgaggcgcc gccccccgaa cgcggcgggt ctccgggaaa cgtagcgcta
     1441 cccaaccgcc gccatcgcga ctcgcgtcgc tcctgcgaaa aacaccacgt tcctggagcc
     1501 gcgctgcagg agcgacgcga gtcgcgagcg gccgtcgggc tgcgacgcag gatgccgcgc
     1561 cgctgcgcgc tcagaccttc aggtcgacct ggctcagcgt gccgacccca ccgtcctcgc
     1621 gcagatagac gccgctcgcg cgcatcacac cctgggtgtc gtgcgcagcg cccttcaggc
     1681 tgaacggtgt cgccacgcgg ccgaggtgga gggcgccgac gcccgcctcg ctcagcgacc
     1741 gcacgctgcc cttgcccgcc gcatccggcc gccacacgcg caactgcgac caggcggcat
     1801 cggcctcgtc gatccagccg ttggcatcgt cgtcgaggcg ggcgagttcg gaaaagccgt
     1861 cgccgctggc gggtccgaag agctcgcggc catcgtcgat gcggccattg gcgttacggt
     1921 cgaaggcgag gaagccgctg ccgctcacca gcggaagctt ctccctggtg ccgtcgctat
     1981 ccagatcgaa gtcgaagcgc acctccgaga gcgccgccgc ggggccgccg aagtcgagca
     2041 ccagtggatc cttcaggcgc tggtcgccgg cgcgcacact gaccgccacc gactcggtgt
     2101 agctgcgcga catctcgaag ccgagctcga aacggatctc ggcaccgtcc gcggtgcgca
     2161 ccacgccggc ggcgccgaag gagatcgtct cggtctcgct gaagcggctg ctgaagtcgt
     2221 attcgagccc gaagccggcg cgccgtgcac cagcctggcc gctcgtaccc gggtttgcgc
     2281 cggtctcata gccaggctcg gcggcgtcgg cagactgcag gtcgcgcatg ctgaagcgcc
     2341 gcaccggctc gccggtgagg aactcgatca tgcggatcag catggcgagg cgcggatcgt
     2401 cttcgactgc ctcctcggcg accgcgtcca ccgccgcagt cgcgctctcg gccgccaggg
     2461 cgttctggcc cgcggcggag agcgccacca ccgggggctg cgtgctctcc gctgcggagc
     2521 cggaatcgcg cgtgcctcgc cagacttcga ggcgctcacg ggtctccagc gactgggcga
     2581 aacggtgcga ggactggagc tcgacactgg acgcggtgac tttcatggct aaggcctcgc
     2641 aaaggtgact gaccggtaca cctaacgaca tcccgtcacg ttccttgacc gcgatcggcc
     2701 gcctcgaagt ccatctgcgg ggaccgacgc ccgccccgct ccgtcgacgg gcgaacgtac
     2761 tcgaagtgca tcagaaattt gttgagcatt catgagcacg ttctatattg aaatgcgctc
     2821 gattgcagtc cacgcatcga tatcgctcgc attcttcgat gacataccgc tcgggagatc
     2881 acgaatgtcc gccccaagag cctatcgatt gctgctcgcc ctcgccgccg cggcgaccgt
     2941 ctccagcagc cacgcagcag ccatcgcctc gcaacccttc gacatcggtg tcgacatggt
     3001 ccaggtattc aacgaccggc tcggcgctgg ccgcttcttt gccggtgcgg gtacggaccg
     3061 cctgcgcgtg agcgcaaggg tgaccccctc gccggatacg gacttctacg atcagagctc
     3121 gaacggcggc gagaccgcga tcttcgtcac gcacccctcc actcctgggc tcgatacccg
     3181 cctgcagttc gtcggcctcc aatccggcgg cggcggcggc aagaacgact acacgacgac
     3241 cttcaatctc gccaatccct tcgtacaggg gcagctcgcc gcctgggacg cgaccccgtt
     3301 cgcgttgcga atcgagaacc cgtccgcccc cggcggcgaa accacgaagt gggtcacagc
     3361 cccggactac gacagcagcg tcaacctgcc cttcctgcaa gatgtcgcga tcaccggcgg
     3421 cggcctcgcc cccaccctgt cgtggaccgt cccggacacc accgcgccga tcaccaacat
     3481 ccgtatccag cttcggcgca tcgacgccga ggagcccggg cgcatcaccg cggcgacgct
     3541 cgtgcacgag gccgtcctgc cgctgggcag caacggctac accctggatc aggccttcag
     3601 caacggcggg cttcccgggc tgccgagcgg cctggagcag gggagcaagt acgaagtggc
     3661 ggtgatcctc gaatccgccg aacccggcct gatcaagggc cgcgcacgca ccttcttcga
     3721 gttcaccccg ctcacggacg acgccggcga caggacgatc tacctgccga gcgtggacag
     3781 tgcgggaaac ttcaacttcg acatcgaggt gagggccggc gagaccatcc tgatcgatcc
     3841 ggtggtcgcg gtcggctatg actatcagat cggcagcggg gatccactgt tcgcaagcgt
     3901 caccctgccg aacgtcggtg acggaatctt cgaactctgg ctttacgacc tgaacctcgc
     3961 cgacttcgtg ttcgaaacct ttctggacgc aggagagcgc ttcgacttca cgggcggcgg
     4021 cgtggatcgg ttccgcgtgc tcggcatcga gccgtcggca ggactggatc cgaccgatgt
     4081 gcaggctttc gtgaccgaga tcggtttcac cgggaatggc cggtttaccg gtcgcatgac
     4141 cccgatcacc accaatttca ctcccgtgcc ggaaccgtcc tcgctcgcgc tgctggccct
     4201 ggcggtgctg gcgctcggag gactgcgacg cagttgccgc gccggaggct cgctgcgcta
     4261 gcatgagcgg attcccgctt cgcccggagt ccgccatgag caacaagcat cgccacaccc
     4321 tggaagcgat cttccgcgat ccgccggccg gcaacctgca ctggcgcgag gtcgagtcgc
     4381 tgctgagcca cctcggcgcc gaggtcgaaa gcggccacgg cgcgcgcttc cgcgtcgtgc
     4441 tgaaccggcg cgagttctac ctgcaccacc cgcaccacgg cagcgacttc ggcaagcagg
     4501 cggtgaagga cctgcgcgaa ttcctcgcca gcgccggcgt gtcgccgtcg agctaccagg
     4561 acggcacggg ctgacgcagc gcgcccgacc gcatcgcggt cgcggggctt cgccgccgcg
     4621 ccacgcgccc ggaggcgtgc gacgcaggaa tccgggcgct ccagccggca ggccgcgcgc
     4681 gtcctcgacg ggcgtcgctc aggcgagcga gcgcgcgagc tccgcgatcg cctcgtcctt
     4741 gtggccgccg tccgcctccg cccactcggg gtgggcgcgg gcgcggccga tcgcctcggg
     4801 caatgcatcc ttgcgccagc gcacgctcac cgggtcggcg atcatcggca tgcctgcgcg
     4861 cccggcatcg agctcgatgg gcttcaccgc ggcgtggccg cgcccgtcca gcgcgaccag
     4921 caggctgcgc tggcgcagcg cgacgatgcg caggatggcg tcgtccacgg tgagctcgcg
     4981 gtggccgcgc agcttggcgg cgaggcgctc tccgtagtga ccgaaggtct gccacaggcc
     5041 gccagcgatg gcgccgaggg cggcggcggc gcccagcgtg aggccgccgg tcatcaggtc
     5101 gatgccgacg ccggctgccg cgcccgcggc ggcgccggtg ccgaggcgta cgcccatctg
     5161 gcgcagggtc tcggggttga agaggtcgtc gtcccagcgc ccgtcggcga gcggcagctc
     5221 ggccatgcgc gcgtcgtcgg cgcggaagcg gtacaggcgc agcagcgcgt ccacgcaggc
     5281 ctgctcgcga ctgcgcacgt ccgcgcgcag gcgctcgacc gcgcgctgca gcgcagcctc
     5341 gtcgtcctcg gaatcggcga ggcggtcacg gcgtgcggcg agctcgacca gcagctcggc
     5401 gaccaggcgg cgggcggctt cgcgacgcgc cgcggcctcg cgcgcacgcg cctcgatcag
     5461 ggcctgcagg cggccgcggt ggctgtggat cagcgtcgcc agggtgtcga agagttcgcg
     5521 ctcgccgtcg agcggcggcg ccacggtgtc gaagctgacc accgcatgca gaccgaggcg
     5581 ggcgagcgcg ccgcgccagt cgtccgcccg cgccaccgga tcggcgatga agttgagtac
     5641 cggcagcagc ggtcgcgcgc acgcggcgag cagctcgagt tcgtcacggt gcttgggcag
     5701 caccggatcg cgcgcatcca ccacatagag cgcagcgtcg ctcgccagca tctggcgcag
     5761 caccttggcc tcctgctcga agcggccggc ggcctcgtcg cgggcgagga agcgcgccac
     5821 gcgctcgggg ccgtcgacac gctcgccggg gtcgaccaga tcgtcgatga actcgagcag
     5881 cgcgatcgcg tcctccatgc ccggggtgtc gaagagctcc gccgccggca cgccgtcggc
     5941 catcaggcgc aggccctcga cgtggcgggt ggtgccggcg gaatcggaga cctcgccgaa
     6001 gcccacgtcg cgcgcaagcg tgcgcaacag cgaggtcttg ccggtgttgg tgtggccgac
     6061 cacggccacg cgcaggatgt cgttcatcgg gcctcgtctc cccgttccag ccagccgcac
     6121 gcggcgcccg cgtccgccag cgctgcctcc tcgcccaggc cgatgccggc caggccctcg
     6181 cgccactggc gcagccgccc ggcccgctcc gccgcggcat cgacgccggc cagccagatg
     6241 cgggtcgcct gcgcgtgatc ggcaagttcg gcgccagcaa ggtgattctg gttccggccg
     6301 gtccg
//
LOCUS       DAOQNN010000172         6265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7056_length_6265_cov_10.234944, whole genome shotgun sequence.
ACCESSION   DAOQNN010000172 DAOQNN010000000
VERSION     DAOQNN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7056_length_6265_cov_10.234944"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="PKV97_10060"
     CDS             complement(<1..1371)
                     /locus_tag="PKV97_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB06261.1"
                     /translation="MKVMQQTAMAPAVREDAVRLARGQFFGEGRAPEAGVSPLIVDSW
                     LRCRGRGMAADDRAEAEHAGRLQLAEAREGNRSLLDYASGVMEHVFEQIRASGSLVIL
                     SDPQGMILHSLGDAEFVDRASRVALQPGACWSEAARGTNAIGTAIVTEAPTVVLGGEH
                     YLDRNGFLSCTAAPIRDPRGALAGVLDVSCEQRMHQRHSLGLVRLSVQLIEKRLFEAE
                     FANHILVAFHERPEYVGSLQEGLVAVTPEGHLAGANTIAREWIARRVECGGGASFAEL
                     FRQGLGKVVDRAMNSSDALIRLGLRDGSEIHVQLRSLRPRFTAFGSAQAGEGMRTFAA
                     EPSPAAVGSAPPVSAPAPVSAPHGPITLHDLATGDAGIGRAVDRARRILGKDIPLLIQ
                     GESGVGKELFAQAFHNSGPRADKTFVALNCAAVPETLIESELFGYVGGAFTGARKEGA
                     LGKIQQA"
     gene            1659..2108
                     /gene="pedF"
                     /locus_tag="PKV97_10065"
     CDS             1659..2108
                     /gene="pedF"
                     /locus_tag="PKV97_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HNB06262.1"
                     /translation="MNTRTIKENLRKRLAITATGIALAAASALAHAHGDVTPQAVDTS
                     SLKQLGGTWVTVNPYRGNKEAIRIGDSAFNQNCARCHGLGAVSGGIAPDLRLLDVADD
                     GTDEFFIGKVRNGVTRNGAVYMPPFEGMLTQEAMWAIRAWLETVHEN"
     gene            2098..2943
                     /locus_tag="PKV97_10070"
     CDS             2098..2943
                     /locus_tag="PKV97_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNB06263.1"
                     /translation="MKTDRRRFLLAAGALAVLAASPARAAAAELELQQPGTLRVAVYA
                     NFAPWSTAGKGIDIALGRALAERLGLQPQVVEFPADENMNDDLRNMVWRGHYLGHKPG
                     DVMLHVPVDPRFAALNKQVKIFGPYHLETIALARLPERVPAPGGSADASFAVFEQERI
                     GAELDSHASDFLRHILGGRLQPNVAHFRNVGEAVAAMKRGEIAAVMGARSELETAIGG
                     DAAIALDTLSMPELRVASWPLGMAVKAEHSGLEQALSGAIAELQRSGELGRIFAAHGV
                     RQRTP"
     gene            complement(3108..3965)
                     /locus_tag="PKV97_10075"
     CDS             complement(3108..3965)
                     /locus_tag="PKV97_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNB06264.1"
                     /translation="MTKLQLSSFATAVVLAVGVGTAAHAAERATPREARTMFEQAVRY
                     MEENGPERAFAAFNDRQGGFVRKDLYVFAIDDKGVYQASGAAPEALVGLKVLDTTDAA
                     GNPLFREMIDATRVSQEATVRYVWLNRASNKVEPKVSYVRKVGQYVVGVGYSAPRASA
                     DDARAMLERAVAVARGEGGARAAARFNDRRGDFVKDDLYVFMVNMESGRFEAMGMNPA
                     LSDTDALGLRDAAGNPLIAEMIARLQQADEATVDYVWRNPVTNAVEHKRSYVRKVGKS
                     LVGVGHYLE"
     gene            complement(4132..5940)
                     /locus_tag="PKV97_10080"
     CDS             complement(4132..5940)
                     /locus_tag="PKV97_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HNB06265.1"
                     /translation="MMKNDRRARTLPAMLTALTVALAAAGMGATAQAASVSDEDILKD
                     AETTHQVVTNGLGTKGQRYSPLAKVNVDTVKDLVPVWAFSFGGEKQRGQQSQPLIHDG
                     KMFVTASYSRIYALDAKTGQKLWKYEHRLPEGIMPCCDVINRGAALYGDLVIFGTLDA
                     QLVALNRDTGKVVWREKIDDYKAGYSMTAAPQIVKGMVITGVSGGEFGVVGRVEARDA
                     KTGKKVWVRPVVEGHMGYKYDKDGKEIENGISGTTNATWPGDLWKTGGAATWQTAYYD
                     PDVNLIFMGTGNPAPWNSWLRPGDNLYSSSTVAVDPDTGKIVWHYQNTPHDGWDFDGV
                     NEFIAFEYKDPKSGKLVKAGAKADRNGFFFVNDRTNGKLLAAYPFLTRIDWAKGYDLE
                     TGRPITNDDKRPPNPFAEGAAGEGGKGKPVFAAPSFLGGKNQMPMGYSPDTGLFYVPA
                     NEWGMDIWNEPVSYKRGAAYLGAGFTIKPLYEDYIGAMRAIDPVSGKIVWEVKNNAPL
                     WGGVLSTAGNLVFYGTPEGYLKAVNAKTGEEAWKFQTGSGVIAPPVTWEADGEQYVAV
                     VSGWGGAVPLWGGDVAKRVNFLEQGGTVWVFKLHKS"
BASE COUNT          928 a         2285 c         2065 g          987 t
ORIGIN      
        1 ggcctgctgg atcttgccga gcgcgccttc cttgcgcgcg ccggtgaagg cgccgccgac
       61 gtagccgaag agctccgact cgatcagggt ctccggcacc gcggcgcagt tgagcgcgac
      121 gaaggtcttg tcggcgcgcg gcccgctgtt gtggaaggcc tgggcgaaga gctccttgcc
      181 cacgcccgac tcgccctgga tcagcagcgg aatgtccttg cccaggatgc gccgcgcacg
      241 atcgaccgcc cgcccgatac ccgcgtcccc ggtggcgaga tcgtggagcg tgatcggccc
      301 gtgcggcgcc gacacaggcg ccggcgccga caccgggggt gcggagccga ccgccgccgg
      361 gctgggttcc gcggcaaacg tgcgcatgcc ctccccggcc tgggccgagc cgaaggcggt
      421 gaagcgcggg cgcagcgagc gcagctgaac gtggatctcg gaaccatcgc gcagtcccag
      481 ccggatcagc gcgtcgctcg agttcatcgc ccggtcgacc acctttccga gcccctggcg
      541 gaacagctcc gcgaacgagg ccccaccccc gcactcgacg cgacgcgcga tccattcgcg
      601 cgcgatcgtg ttggcaccgg ccaggtgccc ctccggcgtc accgccacca ggccttcctg
      661 caggctgccg acgtactccg ggcgctcgtg gaaggcgacc aggatgtggt tggcgaactc
      721 ggcctcgaag aggcgcttct cgatcagctg cacggacaag cgtaccagtc cgagcgagtg
      781 gcgctggtgc atgcgctgct cgcaggacac gtcgagcacg ccggcgagcg cgccgcgcgg
      841 atcgcggatc ggcgcggccg tgcaggacag gaagccgttg cggtcgaggt agtgctcgcc
      901 gccgagcacc acggtggggg cttcggtcac gatcgccgtg ccgatggcgt tggtgccgcg
      961 cgccgcctcg ctccagcacg cgcccggctg cagcgccacc cggctcgccc gatcgacgaa
     1021 ctccgcgtcg cccaggctgt gcaggatcat gccctgcggg tcggacagga tcaccaggct
     1081 gcccgaggca cggatctgct cgaacacgtg ttccatgacc ccactggcgt aatcgagcag
     1141 gctgcggttg ccttcgcgcg cctccgccag ctgcaggcga cccgcatgct cggcttcggc
     1201 gcggtcgtcg gctgccattc cgcgcccgcg gcaacgcagc cacgagtcga cgatgagcgg
     1261 ggacacgcct gcctcgggcg cacgcccttc cccgaaaaac tggcctcgtg ccagccggac
     1321 cgcgtcctcg cggacggccg gcgccatcgc ggtctgttgc atgactttca tgtctcctcc
     1381 gggcagcctt gccacgcgac gtgccggcga ccgcctcttg tgactcttcg acttcgattc
     1441 aaaacgtagc acctgatgcg gatcgataca aatccgctgc tccgccggcg aaggagcaag
     1501 caggaatcct gccaggccga ccgcggcatg gcggccggaa cccggggccg tccatgcctg
     1561 ggcagccgga cgcacatggc acagggattg cagtaggact cccatccggg gtgaaccgga
     1621 gcggatccaa acaccaccac atcgaagagg aagacaaggt gaacactcgt accatcaagg
     1681 agaacctgcg caagcgcctg gccatcaccg cgaccgggat cgcccttgca gccgccagcg
     1741 cgctggccca tgcccacggc gacgtcacgc cgcaggcggt cgacacgtcc tcgctcaagc
     1801 agctcggcgg gacctgggtc acggtgaacc cctaccgcgg caacaaggag gcgatccgca
     1861 tcggcgactc cgccttcaat cagaactgcg cgcgctgcca cggcctcggc gcggtctccg
     1921 gcggcatcgc cccggacctg cgcctgctcg acgtcgccga cgacggcacc gacgagttct
     1981 tcatcggcaa ggtgcgcaac ggtgtcaccc gcaacggcgc ggtctatatg ccgcccttcg
     2041 agggcatgct gacccaggag gccatgtggg ccatccgcgc ctggctggag accgtccatg
     2101 aaaactgatc gccgccgttt cctgctcgcc gccggcgcgc tcgccgtcct tgccgccagc
     2161 ccggcgcggg cggccgcggc cgaactcgag ctgcagcagc cgggcacgct gcgcgtcgcg
     2221 gtctatgcca acttcgcgcc ctggtcgacc gccggcaagg gcatcgatat cgcactcggg
     2281 cgcgcgctcg ccgagcgcct cggcctgcag ccgcaggtcg tggagtttcc cgccgacgag
     2341 aacatgaacg acgacctgcg caacatggtc tggcgcggcc actacctcgg ccacaagccg
     2401 ggcgacgtca tgctgcacgt gcccgtcgac ccgcgcttcg cagcgctcaa caagcaggtg
     2461 aagatcttcg gcccctacca cctcgagacc atcgcgctcg cccgcctgcc cgagcgcgtg
     2521 cccgcaccgg ggggctcggc cgacgccagc ttcgcggtgt tcgagcagga acgcatcggc
     2581 gccgagctcg actcgcatgc gagcgacttc ctgcgccaca tcctgggcgg gcgcctgcag
     2641 cccaacgtcg cgcacttccg caacgtgggc gaggcggtgg cggcgatgaa gcgcggcgag
     2701 atcgccgcgg tgatgggcgc gcgctccgag ctggagacgg cgatcggcgg cgacgccgcg
     2761 atcgcgctcg acaccctgtc gatgcccgag ctgcgcgtcg cctcctggcc gctcggcatg
     2821 gccgtcaagg cggagcacag cggcctggag caggccttgt cgggggcgat cgccgagctg
     2881 cagcgctccg gcgagctggg caggatcttc gccgcgcacg gggtcagaca gcgcaccccc
     2941 tgatccttcg gggcagccgg gctcgcacgc ccacgccgcc ccagccgcgc agattcgctc
     3001 cgacaatcca cccgccaatg aagagggccc gcatcgcggg cccttttttc tgctccacat
     3061 tgctccacca cggagcatgt gctccatggt ggagcaacac cctgcactta ctcgagatag
     3121 tgccccacgc cgaccaggga cttgcccacc ttgcggacgt aggatcgctt gtgttccacc
     3181 gcgttggtga ccggattgcg ccacacgtaa tcgacggtgg cctcgtccgc ctgctgcagc
     3241 cgggcgatca tctcggcgat caggggattg ccggcggcgt cgcgcaggcc gagcgcgtcg
     3301 gtgtccgaga gcgcggggtt catgcccatc gcctcgaagc ggccgctctc catgttcacc
     3361 atgaacacgt agaggtcgtc cttgacgaag tcgccacggc ggtcgttgaa gcgcgccgcg
     3421 gcccgcgcgc caccctcgcc gcgcgccacc gcaaccgcac gctcgagcat cgcgcgcgca
     3481 tcgtccgcgc tcgcgcgcgg cgccgagtag cccaccccga ccacgtactg accgaccttg
     3541 cgcacatagc tgaccttggg ctcgaccttg ttgctcgcgc ggttcagcca cacgtagcgc
     3601 accgtcgcct cctggctgac gcgggtggcg tcgatcatct cgcggaacag cggattgccc
     3661 gccgcgtcgg tggtgtcgag caccttcagc ccgaccagcg cctcgggcgc cgcgccgctg
     3721 gcttgataca cacccttgtc gtcgatcgcg aagacataga ggtccttgcg cacgaacccg
     3781 ccctggcgat cgttgaaggc cgcgaacgcg cgctcggggc cgttctcctc catgtagcgc
     3841 acggcctgct cgaacatcgt gcgcgcctcg cgcggtgtcg cgcgctcggc cgcatgcgcg
     3901 gccgtaccga cgccgaccgc gagcacgacc gcggtggcga agctggatag ctgtaacttt
     3961 gtcatcggaa atgctccatg ttgccgaaaa acctgcctct ttctcgcggc gcgaccgcag
     4021 ccacgcatgc ggcgtcccga cacgacacgg cccgggcgcc atcgcgtccg ggccgcgggg
     4081 agtgcggcgc cgcggcctgc ggccccccgg agggcgccgc aggcaacgcg atcagctctt
     4141 gtgcagcttg aacacccaca cggtgccgcc ctgctcgagg aagttcaccc gcttggcgac
     4201 gtcgccgccc cacagcggta ccgccccgcc ccagcccgac accaccgcga cgtactgctc
     4261 gccgtcggct tcccaggtca ccggcggcgc gatcacgccc gagccggtct ggaatttcca
     4321 cgcctcctcg ccggtcttcg cgttgaccgc cttcaggtag ccctcgggcg tgccatagaa
     4381 caccaggttg ccggcggtgc tgagcacccc gccccacagc ggcgcgttgt tcttgacctc
     4441 ccagacgatc ttgccgctca ccgggtcgat cgcgcgcatc gcgccgatgt agtcctcgta
     4501 cagcggcttg atggtgaagc ccgcgcccag gtaggccgcg ccgcgcttgt agctcaccgg
     4561 ctcgttccag atgtccatgc cccactcgtt ggccggcacg tagaacaggc cggtgtccgg
     4621 gctgtagccc atcggcatct ggttcttgcc gccgaggaag gacggcgcgg cgaacaccgg
     4681 cttgcccttg ccgccctcgc cggccgcgcc ctcggcaaac ggattgggcg gacgcttgtc
     4741 gtcgttggtg atcggacgac cggtctcgag gtcgtagccc ttggcccagt cgatgcgggt
     4801 caggaagggg taggcggcga gcagcttgcc gttggtccgg tcattgacga agaagaagcc
     4861 gttgcggtcg gccttggcgc ccgccttgac gagcttgccg ctcttcgggt ccttgtactc
     4921 gaaggcgatg aattcgttca ccccgtcgaa gtcccagccg tcgtgcggcg tgttctggta
     4981 gtgccagacg atcttgccgg tgtccgggtc gaccgcgacc gtggacgacg agtacaggtt
     5041 gtcgcccgga cgcagccagg agttccacgg cgccgggttg ccggtgccca tgaagatcag
     5101 gttcacgtcc ggatcgtagt aggcggtctg ccaggtcgcg gcgccgccgg tcttccacag
     5161 gtcgcccggc caggtggcgt tggtcgtgcc cgagatgccg ttctcgatct ccttgccgtc
     5221 cttgtcgtac ttgtagccca tgtggccttc gaccaccggg cgcacccaga ccttcttgcc
     5281 ggtcttggcg tcgcgcgcct cgacgcggcc gacgacgccg aactcgccgc ccgacacccc
     5341 ggtgatcacc atgcccttga cgatctgcgg cgcggcggtc atcgagtagc cggccttgta
     5401 gtcgtcgatc ttctcgcgcc acaccacctt gccggtgtcg cggttgagcg cgacgagctg
     5461 cgcgtccagg gtgccgaaga tcaccaggtc gccgtagagc gcggcgccgc ggttgatcac
     5521 gtcgcagcac ggcatgatgc cctcgggcag gcggtgctcg tacttccaca gcttctggcc
     5581 ggtcttggcg tcgagcgcgt agatgcggct gtaggacgcg gtcacgaaca tcttgccgtc
     5641 gtggatcaac ggctgcgact gctggccgcg ctgcttctcg ccgccgaagg agaaggccca
     5701 caccgggacg aggtccttca cggtgtcgac attgaccttg gcgagcgggc tgtagcgctg
     5761 acccttggtg ccgaggccgt tggtgacgac ctgatgcgtc gtctcggcat ccttcaggat
     5821 gtcctcgtcg ctcacgctcg cggcctgcgc cgtggcgccc atgcccgccg cggccagcgc
     5881 gaccgtcagg gcggtcagca tcgccggcag ggtgcgcgcg cgtctgtcgt tcttcatcat
     5941 cgactcctcc ttggatttct ttctaagctt ttccgggtgg tgtgacttcc ggcaccaact
     6001 cgtgccggac cacgacccca ccgcaaagcc cgtgccaggg ccacgaaccc atgcgctcgc
     6061 gcccgcgctg ctccaacgcg ggcgcattgc ggacggacac tgtggcggcg gagcacagga
     6121 tgttgcggat cggaacaccc gggatccggg cgcaatgctc cgcggctgcg caggtccgca
     6181 tccgaggccc gcccgcggaa ttggcatgtg gcttgcatcc acgctccggc acacgcaggc
     6241 gcacgcaccg cgcccggccc acagg
//
LOCUS       DAOQNN010000173         6257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7073_length_6257_cov_10.461948, whole genome shotgun sequence.
ACCESSION   DAOQNN010000173 DAOQNN010000000
VERSION     DAOQNN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7073_length_6257_cov_10.461948"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PKV97_10085"
     CDS             complement(<1..673)
                     /locus_tag="PKV97_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNB06266.1"
                     /translation="MIPSDLAARLRTLTEASFFETEPPVPGLQRAREIQARLPELVPG
                     QRFLATLQRTLPDGSFRAVVAGQQMTLALDGSAKAGDTLELEVTQLTPRTVFARVVGG
                     EAATTAGASAQPALSQTGRLISFLLTGQPVPSPASLAGNRPLLDAPPTAGSQLVPVLR
                     QALGQSGLFYESHQVQWVLGKLETAALLREPQGQAGGANTAGGGAGASAGGAAAAAAG
                     GAAASG"
     gene            complement(717..1064)
                     /locus_tag="PKV97_10090"
     CDS             complement(717..1064)
                     /locus_tag="PKV97_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HNB06267.1"
                     /translation="MLTLPTLAALLERYEAMAEAARANDWERLSALEREAAALREGAR
                     GDTSGAMDDAAALARLPPADAAELREGIARLLALDAEIRSHTDPFLSSVRKLLSAGRQ
                     ERALRDAYGASSR"
     gene            complement(1323..1733)
                     /gene="fliS"
                     /locus_tag="PKV97_10095"
     CDS             complement(1323..1733)
                     /gene="fliS"
                     /locus_tag="PKV97_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNB06268.1"
                     /translation="MFGGFGNRAAAYAKVSVETGVNTADPHKLILMLFDGALLQVRTA
                     GIAIGNQDIPGKGTAISKAIEIIINGLKVSLDMNAGGELAQRLAALYDYMSERLLYAN
                     LHNSQPALDEVAGLLATLREAWAEIRSQVQPSAA"
     gene            complement(1775..3118)
                     /gene="fliD"
                     /locus_tag="PKV97_10100"
     CDS             complement(1775..3118)
                     /gene="fliD"
                     /locus_tag="PKV97_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNB06269.1"
                     /translation="MAISAAGVGSGLDVGSILNQLMAVERQPLSRLQEQQKSYQSKLS
                     AFGQLQSAMSKLQDAATALTKSSTFSATTASAGDTKAFTVSSTASAQTGSYNIEVSQL
                     ARAQRTATSATTAPDLSGGGSIDITLGTGTPKTINLAAGGTLQDLRDAINAADAGVSA
                     QIVNNGTVNQLVISSKETGAASAFKLEGFGGLSEFSFDPAAPAGDMVSVQQAKDAMLS
                     IDGLAITRSSNTVSDAIEGVTLTLAKPTESETTMTVARNDETAKKAIDDFAKAYNELN
                     SLIRSQTSYNAETKKAGTLNGDTGVRSIQSQLRGVFSNPISGLSGATMLSDAGISIRT
                     DGSMSVDSTKLASALADPSKKIAELFAGNGTVDGFAKTLETRIKDMLATDGLLTSRTD
                     GINRTIKSFDSRIEAFELRLEKVQARYSAQFTALDAAMSSMSTTSAYLTQQLANL"
     gene            complement(3138..3518)
                     /locus_tag="PKV97_10105"
     CDS             complement(3138..3518)
                     /locus_tag="PKV97_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNB06270.1"
                     /translation="MSMQAMTAAPVRDAQAARNQADAPAGGSRAAEARGEIIQQNISP
                     QDQNPRTPDIGEVQKALEEVEKAVAPMAQSLQFSLDKDSGRTVVKVMDTDTNEVIRQI
                     PSEEVLAISKAVDKLKGLLLKQQA"
     gene            complement(3628..4464)
                     /locus_tag="PKV97_10110"
     CDS             complement(3628..4464)
                     /locus_tag="PKV97_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNB06271.1"
                     /translation="MTQTINTNVASLNAQRNLTSSQSSLGTTLQRLSSGLRVNSAKDD
                     AAGLAIAERMNSQVRGMNVAIRNSSDAISLSQTAEGALGKIGDSLQRMRDLAVQSANG
                     TNTSGDRDNLQLEFRQLQDEILRVTKDTKFNNQAVLAGTNFTFQVGANTGETIGVSAT
                     AVSATNIQSAAMTGASAIAVSGTDASSATAAIAAIDLAISEVVKQRATFGAAQTRFES
                     VISNLQVASENQAAARGRIMDADFAAETANLSRTQILQQAGTAMLAQANSLPQNVLSL
                     LR"
     gene            complement(4693..>6257)
                     /locus_tag="PKV97_10115"
     CDS             complement(4693..>6257)
                     /locus_tag="PKV97_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 2 glycosyl transferase"
                     /protein_id="HNB06272.1"
                     /translation="LGIAQRPEVGIVGPRLAFAETAKIQDGGWILGLNGLAATPWNCE
                     MELTEPGYMGRAVCDQHVGAVSGSALLVRASLFDDLCGFNETDFPQFNGALDLCLRVS
                     ERGLQIVWTPYSMLVHYGGVSTHERRKDIASALEDVIAGKVEREGILQRWLPVLASDP
                     GYNKNLSLVETFKPDHIAPIVWDTNFHDRSRILGIPLSGGAGEYRLRAPLRIIARAGL
                     AQTAICEPPAHLSVRVLSPIEIARTAPDSIIFHQPVDDHQTNTLQSLANLLPRVRRII
                     TIDDLFTAVPRKNSFYKFQYKDARPRLRRTLGLADKLVVSTQPLADFCSSMIEDIRIM
                     PNCLERAIWDGARPPALPRRKPRVGWAGAQQHLGDLEIIYPVVEALSEEVDWIFMGMC
                     PDPLRPFVREFHDFVRDFEAYPAALAKLDLDLAIAPLELNAFNEAKSNLRLLEYGFMG
                     WPVICTDIFPYQDAPVTRLPNEPQRWISAIREHLAEPEAMRAAGKALQEWVLANFILE
                     DHATDWLEAYGP"
BASE COUNT          977 a         1921 c         2129 g         1230 t
ORIGIN      
        1 gtccgctcgc agcagccccg cctgctgcag ccgccgccgc gcctcccgcg ctcgcacccg
       61 ctccgcctcc cgccgtgttc gccccgcccg cctgcccctg cggctcgcgc agcagcgcgg
      121 cggtttcgag cttgccgagc acccactgca cctggtgcga ttcgtagaac agcccgctct
      181 gcccgagcgc ctggcgcagc accggcacga gctggctgcc ggcggtggga ggagcgtcga
      241 gcagcgggcg gttgccggca aggcttgccg gcgagggcac cggctggccg gtgagcagaa
      301 agctgatcag gcgcccggtc tggctcagcg ccggttgcgc cgaggcgccg gcggtcgtgg
      361 ccgcctcgcc gcccaccacg cgcgcgaaca ccgtgcgcgg ggtgagctgg gtgacctcga
      421 gctcgagcgt gtcgccggcc ttggccgagc cgtcgagcgc cagcgtcatc tgctgtccgg
      481 ccaccacggc gcggaagctg ccgtcgggca gggtgcgctg caaggtggcg aggaagcgct
      541 ggccgggcac gagctcgggc aggcgggcct ggatctcgcg tgcgcgctgc aggccgggga
      601 ctggcggctc ggtctcgaag aagctcgctt cggtgagcgt gcgcaggcgg gcggcgaggt
      661 cggaggggat catcgcatgc gctccatcgg gcccggccgg tcagggcgcg tcggcctcag
      721 cgcgaggacg cgccgtaggc gtcgcgcagc gcgcgctcct ggcgcccggc ggagagcagc
      781 ttgcgcacgc tggagaggaa gggatcggtg tggctgcgga tctcggcgtc gagcgccagc
      841 aggcgcgcga tgccctcgcg cagctcggcg gcgtccgccg gcggcagcct ggccagcgcg
      901 gctgcgtcgt ccattgcgcc cgacgtatcc cctcgcgccc cctcgcgcag cgccgcggcc
      961 tcgcgctcga gcgccgaaag gcgctcccag tcgttcgcac gcgccgcctc ggccatcgct
     1021 tcgtagcgct cgagcagggc ggcgagggtg ggcagggtca gcatggcgga agaggactca
     1081 tcgggaagcg gttcgatcat cgacaacaac gcagcacgcg cctgtaggag cgacgcaagt
     1141 cgcgatcacc cccgtcacga aatgcactcc gcgcccacca ccaccggcgg cgatccttcg
     1201 cccggctcgt ctatcgcacg ggcagcggcg attgggattg cggttgcttc gcgacttgcg
     1261 tcgctcctac gaaaagcaag aagcaacaaa tgccgatgca agaaatgcgc ctgtccgcag
     1321 cctcacgccg cgctcggctg cacctggctg cggatctccg cccaggcctc gcgcagcgtc
     1381 gccagcaaac ccgccacttc gtcgagcgcg ggctggctgt tgtgcaggtt ggcgtagagc
     1441 aggcgctcgc tcatgtagtc gtagagtgcg gcgaggcgct gcgcgagctc gccgccggca
     1501 ttcatgtcga ggctgacctt gaggccgttg atgatgatct cgatcgcctt ggagatcgcc
     1561 gtgcccttgc cgggaatgtc ctggttgccg atcgcgatgc cggcggtgcg cacctgcagc
     1621 agggcgccgt cgaacagcat caggatcagc ttgtgcgggt cggcggtatt gacgccggtt
     1681 tcgacgctga cctttgcgta ggcggcggcg cggttaccga aacctccaaa cattttttct
     1741 tctctcctgg aaacgctcgt ccgaagtgtg cggcttagag gttggcgagt tgctgggtca
     1801 ggtaagcgct ggttgtgctc atgctcgaca tggcggcatc gagtgcggtg aactgcgcgc
     1861 tatagcgcgc ctgcaccttt tcgagccgca actcgaatgc ttcaatgcgg gaatcgaagg
     1921 acttgatcgt gcgattgatg ccgtcggttc gtgacgtcag caggccatcc gtcgcaagca
     1981 tgtccttgat gcgggtttcc aaggtcttgg caaagccatc cacggtgccg ttgccggcaa
     2041 agagctcagc gatcttcttc gaggggtcgg caagggctga agcaagcttg gtgctgtcga
     2101 ccgacatgct cccgtccgtc ctgatgctga ttccggcatc ggacagcatg gtcgcgccac
     2161 tcaggcccga gatcggattc gagaagacgc cgcgaagctg gctctggatc gaccgcacgc
     2221 cggtgtcgcc gttcaaggtg ccggccttct tggtctcggc gttgtagctg gtctgactgc
     2281 ggatcagcga attgagctcg ttgtaggcct tggcgaaatc gtcgatcgct ttcttggccg
     2341 tctcgtcgtt gcgggccacg gtcatggtgg tttcgctctc ggtcggcttg gcgagtgtta
     2401 gcgtcacgcc ctcgatcgca tcgctcaccg tgttgctgga acgggtgatc gcaaggccgt
     2461 cgatgctcag catcgcgtcc ttggcctgct ggaccgagac catgtcgcca gcaggtgccg
     2521 cggggtcgaa gctgaattcg ctcaatccgc cgaagccctc gagcttgaag gcgctggctg
     2581 cgccggtttc cttgctgctg atgacgagct gattgaccgt gccgttattg acgatttgag
     2641 cactgacccc ggcatcggcg gcattgatcg catcgcgcag gtcctgcagc gtacccccgg
     2701 ccgcgaggtt gatcgtcttc ggcgtgcctg tgccgagggt aatgtcgatc gagccaccac
     2761 cactgagatc gggtgcggtt gtggcgctcg tggcagtgcg ctgcgcgcgg gcgagctggc
     2821 tgacttcgat gttgtagctg cccgtctgcg ccgaggcggt cgagctgacc gtgaacgcct
     2881 tggtgtcgcc cgcgctcgcc gtcgtggccg agaaggtgct cgacttggtc agcgccgttg
     2941 cggcgtcctg cagcttggac atggcgctct gcagttgccc gaaggccgag agcttggatt
     3001 gatagctctt ctgctgctcc tgcaggcgac tcagcggctg gcgttcgacc gccatcaact
     3061 ggttgaggat gctgccgacg tccagccccg agccgacccc tgctgcgctg attgccatgt
     3121 tgctaccctc ctgccgctca ggcctgctgc ttgaggagca gacccttcag cttatcgacc
     3181 gccttcgata tggcgagcac ttcttcggac ggaatctggc ggatgacctc gttggtgtcg
     3241 gtgtccatga ccttgaccac ggtcctgccg gaatccttgt cgagcgagaa ctgcaggctc
     3301 tgcgccatgg gcgcgaccgc cttctcgacc tcctcgaggg ccttttgcac ctcgccgatg
     3361 tcgggagtgc gcggattctg gtcctgtgga ctgatgttct gctggatgat ctctccgcgt
     3421 gcttccgcgg cacgggagcc gccggcgggc gcatcggcct ggttgcgtgc cgcttgggca
     3481 tcgcgaaccg gcgcggcggt catcgcctgc atcgacatcg acgcgttgtt gatgggttgg
     3541 attgacatga ttcgacctcc tgatgggcca acggcgcgaa gcgtattcta cgctccgcgc
     3601 cgatcggttc atgacgcttg ccgagaatca gcggagcagg gacagcacgt tctgcggcag
     3661 cgagttcgcc tgcgccagca tcgcggtacc ggcctgctga aggatctggg tgcgcgacag
     3721 gttcgcggtt tcggcggcga agtcggcgtc catgatgcgg ccgcgggcgg cagcctggtt
     3781 ctcggaggcg acctggaggt tcgagatcac ggactcgaag cgagtctgcg ctgcaccaaa
     3841 ggttgcgcgt tgtttgacca cctcggaaat cgcgaggtcg attgctgcga tggcagccgt
     3901 tgccgatgag gcatccgtgc cactcactgc gattgcgctg gcgccggtca tcgctgcgct
     3961 ctggatgttc gtcgccgaga ccgcagtcgc ggagacgcca atggtttcac ccgtgtttgc
     4021 cccgacctgg aaggtgaagt tcgtgccggc aaggaccgcc tggttgttga acttcgtgtc
     4081 tttggtcaca cgaaggatct catcctgaag ctgccggaat tcgagttgca ggttgtcacg
     4141 gtcgccggaa gtgttggttc cgtttgccga ctggacagcg aggtcgcgca tgcgttgcag
     4201 ggaatcaccg atcttgccca gcgcaccttc ggccgtctga gagagcgaga tggcgtcgct
     4261 ggagttgcgg attgcgacgt tcatgccgcg cacctgggag ttcatgcgtt cggcgatcgc
     4321 gagaccggca gcgtcgtcct tggcgctatt cacccggagg ccggaggaga ggcgctgcag
     4381 ggtcgtgccc agcgagctct gggaggaggt gaggttacgc tgcgcgttga gcgaggcgac
     4441 gttggtgttg atcgtctggg tcatgatttt tctcctggga tatcaggttg gtcattcacc
     4501 cgcaggttgc gtcggttgcc tcgtcgggtg cggagcgttt gtcgtgctct tgaatgctgt
     4561 tacggcaacg gtgctctgct tcttgagtgc tttttcgatc ttccgactgt cgtgcgctgg
     4621 atccgccgat gtcttcggcg cgcggggtcg gtcgtgggcc gatcctgcgc gccgtccggg
     4681 gcgcgcaaag gctcagggac cgtaggcctc gagccagtcg gtcgcgtgat cctcaaggat
     4741 gaagttggcg aggacccatt cctgtagtgc cttgccggca gctcgcatgg cttcgggttc
     4801 ggccaaatgc tcgcggatcg cggaaatcca ccgctggggc tcgttcggca ggcgggtcac
     4861 cggcgcatcc tgataaggga agatgtcggt gcagatcacc ggccaaccca tgaagccata
     4921 ctcgagcagt cgcaggttgc tcttcgcctc gttgaaggca ttcagctcga gtggcgcgat
     4981 cgcgagatcc agatcgagct tggcgagtgc cgcgggatag gcttcgaagt ctcgaacgaa
     5041 gtcgtgaaac tctcgcacga aaggacggag agggtcgggg cacatcccca tgaagatcca
     5101 atccacttct tccgaaaggg cctcgacgac cggatagatg atctcgagat cgccaaggtg
     5161 ctgctgggca cccgcccatc ccacccgggg tttgcgtcgt gggagcgctg gcggccgggc
     5221 tccgtcccag atcgctctct cgaggcagtt gggcatgatg cggatgtctt cgatcatgct
     5281 cgagcagaag tctgcaagcg gctgggtgct gacgaccagt ttgtcggcca agcccagcgt
     5341 tctccgcagg cgggggcggg catccttgta ttgaaacttg tagaacgagt tcttcctggg
     5401 gaccgcggtg aacaagtcat cgattgtgat gatccgacgt acccgaggga ggagattggc
     5461 cagactctgt agtgtgttcg tctgatggtc gtcgaccggt tgatggaaaa tgatcgaatc
     5521 cggtgcggtc cgggcgatct cgataggtga gaggactcgg acgctcagat gagcgggagg
     5581 ctcgcagatc gcggtctgcg ccaacccggc acgcgcgatg atgcgcaagg gcgcgcgaag
     5641 acggtactcg ccagccccgc cgctcaatgg gatgcccagg atccgcgagc gatcgtggaa
     5701 attggtgtcc cacacgatcg gcgcgatgtg atctggcttg aatgtctcga cgaggctgag
     5761 attcttattg tacccggggt cgctggcgag cacggggagc catctttgca gaatgccttc
     5821 acgctcgacc ttgcctgcaa tgacgtcttc gagcgcactg gctatgtctt tccgacgctc
     5881 gtgggtcgat acgcctccgt agtgtacaag catcgagtag ggcgtccaca cgatctggag
     5941 gcctctttcc gaaacgcgaa ggcagaggtc aagcgctccg ttgaattgcg gaaaatcggt
     6001 ttcgttgaag ccgcataagt cgtcgaaaag ggatgcacga acgagcaatg cggagccgct
     6061 caccgccccc acgtgctgat cacagactgc acgacccatg tacccgggct cagtcaattc
     6121 catttcgcag ttccaagggg tcgccgcgag gccattcagc ccgaggatcc agccgccatc
     6181 ctgaatcttc gcggtctcgg cgaaagcaag gcgcggtccg acaataccga cctccgggcg
     6241 ctgcgcgatg cctagca
//
LOCUS       DAOQNN010000174         6231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7124_length_6231_cov_10.644916, whole genome shotgun sequence.
ACCESSION   DAOQNN010000174 DAOQNN010000000
VERSION     DAOQNN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7124_length_6231_cov_10.644916"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..914)
                     /locus_tag="PKV97_10120"
     CDS             complement(207..914)
                     /locus_tag="PKV97_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNB06273.1"
                     /translation="MPEINYLWTPPAPVSLPVRGTGARFPVNRLFFVGRNYQAHAIEM
                     GSSVDKASMRPVYFTKSSANLALSGGTAPYPPETRDYHHEMELVVAIGEPGFRVSESS
                     ARRMIYGYACGLDMTRRDLQATAKARGNPWDLAKNVESGAVCGEIQPAAGQVLRNGEI
                     VLRVNGEVRQRADLSDMIWSIPEIIADLSSYYHLQPGDLIYTGTPEGVGPVRPGDRLE
                     GWVQGAGKIEFEIGQAE"
     gene            1078..5013
                     /gene="purL"
                     /locus_tag="PKV97_10125"
                     /gene_synonym="purI"
     CDS             1078..5013
                     /gene="purL"
                     /locus_tag="PKV97_10125"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNB06274.1"
                     /translation="MTSILKLRGAPALSSSRLERLSRAVGEVLPKLAGLAAEHWYFVE
                     VSAALDEAERARLVDLLDAGPASTAAPTGTLRLVVPRLGTISPWSSKATDIAHQCGFD
                     KIVRIERGIAYSIDARGVDGNTALAALLHDRMTESVLDSMQAAEALFHHYQPQPLTTV
                     DILAGGRAALERANGELGLALSEDEIDYLVENFSRMGRNPTDVELMMFAQANSEHCRH
                     KIFNADWVIDARPMEKSLFGMIKDTHKAHPEGTVVAYSDNASVIEGARIARLYPDSDG
                     AFRYHDEDTHILAKVETHNHPTAISPFPGAATGAGGEIRDEGATGRGSRPKAGLAGFT
                     VSNLNIPDFGQPWEKPYGKPERIASALDIMIEGPIGAAAFNNEFGRPNLAGYFRTFEQ
                     AVQGEVRGYHKPIMIAGGLGSIQARQAEKPTFPPGTLLIQLGGPGMLIGLGGGAASSM
                     ATGTNTADLDFASVQRGNPEIQRRCQEVIDACWQQGENNPIIAIHDVGAGGLSNAMPE
                     LADHAGLGAHFELREVHIEEPGMSPREIWSNESQERYVLAIAPESLPMFQAFCERERC
                     PFAVLGTATADGHLTVSDRHFGNKPVDMDMKVLLGKPPKMTRNVSRRAVHLPPFDTTG
                     FDLQEAGMRVLRVPAVASKSFLITIGDRSVGGLTARDQFVGPWQVPVADVAVTAMSFQ
                     GYRGEAFAMGERTPLACVDAPASGRMAIGEAITNIAAADIEKLGDVKLSANWMAAAGH
                     RGEDARLYDTVKAVSEFCVSAGLSIPVGKDSLSMRTAWRDGEEDKQVVAPLSLIATAF
                     APVQDIRSTLTPQLQLHEGVETELLLIDLGNGKNRLGGSAFAQAYESVGEHAPDVDPA
                     QLAAFFETVQRLRRDGLLLAYHDRSDGGLFATVCEMAFAAKCGLSLILDTVCYDPYMM
                     DVDGLEKKPDTLKGRFDDRLFAGLFAEELGAVIQIRRDDRSRITEVLRAARLAYHFIG
                     EPNDKDEIRFRRNAKLVFAASRVELLQAWSETSYRVAKLRDDPESVQQEFDALADAGN
                     PGLSVALSFDVKEDVAAPFIASTARPKVVVLREQGVNSQFEMAAAFERAGFTPVDVHM
                     SDLQAGRIDLADFHGLAACGGFSYGDVLGAGQGWAKSILFNPRLRDEFAAFFGRSDTF
                     ALGVCNGCQMMAHLAPIIPGAEAWPTFHRNRSEQFEARFVMVEVADSPSILLQGMAGS
                     RMPIVVSHGEGRAVFHSEADRERALLALRYVDNHGNPAQTYPANPNGSPEGVTGFTTA
                     DGRFTIMMPHPERTARTLQMSWAPQWLVTDSPDASPWLRMFRNARLWLG"
     gene            5171..>6231
                     /locus_tag="PKV97_10130"
     CDS             5171..>6231
                     /locus_tag="PKV97_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNB06275.1"
                     /translation="MFVVAIDRPWTETPFMMQGFLLENVDQLRALQHYCRSVTIDRTR
                     SIGDQYSAKVFDKDAPLRGADGFVRKHPDVTESRVGAQRFIKVARGFKGKIHTLRQPH
                     VPRIRIEDGRSRLESELLYSAPIVDDVFRALRETQLAIDSNVAIDVPQISGLVGEMAA
                     GVQRNPDALLWLTRLKRTDQYAYDHAVNVSVHAMVFARFLGLSGKQVHQLGLAGLLQD
                     IGKVQIDYGLLSKPGPLDEREFEHVKAHVTHTVRLLQAHRDFDAEVLATIAAHHERFD
                     GTGYPRGLEGMSIPLPAEMAGITDAYCAMTRDRVYREAVSSQRAMETLNQLRNTQFRD
                     TLVDQFLQCIGLYPIGTLV"
BASE COUNT         1017 a         2191 c         2027 g          996 t
ORIGIN      
        1 aacgccccac gcacacatcc gtgcctgggg cgtcgtgccg tctacgcgga acgcaacggc
       61 agcacgcgac gccagcgccg ggcgccgacc cgggctgcga acgaagccag cctgcaaacg
      121 ttacggtgca ggaccgccgc aaggcaagaa tgcggccgtg acggcatgac gactacgccg
      181 aggtgacggc ggacttcctc cttggcttat tcggcctggc cgatctcgaa ctctattttg
      241 cctgcccctt gcacccagcc ttccaggcga tctcccggtc ggacggggcc gacgccttcc
      301 ggtgtgccgg tgtagatcag atcgccaggc tgcaggtggt agtaggacga cagatcggcg
      361 atgatctccg ggatgctcca gatcatgtcg gatagatcgg cacgctggcg gacttcacca
      421 ttgaccctta gcacgatctc cccgttgcgc aggacctggc cggcggcggg ctggatctcg
      481 ccgcacacgg ccccactctc cacgttcttg gcgagatccc agggattgcc cctggccttg
      541 gccgtggcct gcaggtcgcg ccgggtcatg tcgaggccgc aggcatagcc gtagatcatg
      601 cgccgggcgg aggattcgct gacgcggaaa ccgggctcgc cgattgcaac cacgagttcc
      661 atctcgtgat ggtagtcgcg ggtttcgggt gggtaaggcg ccgtaccgcc cgagagggcg
      721 aggttggcgg aggacttggt gaagtacacc gggcgcatgc tggccttgtc gaccgagctg
      781 cccatctcga tcgcgtgggc ctggtagttg cggccgacga agaacaggcg attgaccggg
      841 aagcgggcgc cggtaccgcg gacgggcagg gacacgggcg ccgggggcgt ccacaggtag
      901 ttgatttcgg gcatttcggg cctcttgacc atcgagccgc ggagtctaca acgaccgggg
      961 cggccgaagg ggctcggtta taatgcgggc ctgcccgcgg acaccccatc ggggatcacg
     1021 gtggcctgta ctggcgcctg ttccgcactg cccgtatccg acccggttct cgagaacatg
     1081 acttcgatcc tcaagctgcg tggcgccccg gcgctgtcct cctctcgtct ggaacgtctt
     1141 tcccgcgccg tcggcgaggt gttgcccaag ctggccggac tggccgcgga gcactggtac
     1201 ttcgtcgagg tctcggctgc gctggacgag gcggagcgtg cgcgtctggt ggatctgctc
     1261 gacgccgggc cggcaagcac ggcagccccc accggcacgc tgcgcctggt ggtgccgcgc
     1321 ctgggcacga tctcgccgtg gtcgtccaag gccaccgaca tcgcgcacca gtgcggtttc
     1381 gataagatcg tgcgcatcga gcgcggcatc gcctattcga tcgacgcgcg cggggtcgac
     1441 ggcaacaccg cgctggcggc gttgctgcac gaccgcatga ccgagtcggt gctcgacagc
     1501 atgcaggcgg ccgaggcgct cttccaccac taccagccgc agccgctcac gaccgtggac
     1561 atcctcgccg gtggtcgcgc cgcgctcgag cgtgccaacg gcgagctcgg cctcgcgctg
     1621 tccgaagacg agatcgacta cctggtggag aacttcagcc gcatggggcg caaccccacc
     1681 gacgtcgagc tgatgatgtt cgcccaggcc aactccgagc actgccggca caagatcttc
     1741 aacgccgact gggtgatcga cgcgcggccg atggagaagt cgctgttcgg catgatcaag
     1801 gacacgcaca aggcccaccc cgagggcacg gtggtggcct actcggacaa cgcctcggtg
     1861 atcgagggcg cgcgcatcgc ccgcctgtac ccggactcgg acggcgcgtt ccgctaccac
     1921 gacgaggaca cccacatcct cgcgaaggtc gagacccaca accacccgac cgcgatctcg
     1981 cccttcccgg gcgcggccac cggcgccggc ggcgagatcc gcgacgaggg tgcgaccggc
     2041 cgcggctctc gtcccaaggc cggcctggcc ggcttcacgg tttccaacct caacatcccc
     2101 gactttggcc agccctggga gaagccctac ggcaagcccg agcgcattgc ctccgcgctc
     2161 gacatcatga tcgagggccc gatcggcgcc gccgccttca acaacgagtt cggccgcccc
     2221 aacctcgccg gctacttccg caccttcgag caggcagtgc agggtgaggt gcgcggctac
     2281 cacaagccga tcatgatcgc cggcggcttg ggtagcatcc aggctcggca ggccgagaaa
     2341 cccaccttcc cgccgggcac gctgctgatc cagcttggcg gcccgggcat gctcatcggc
     2401 ctgggtggcg gcgcggcctc gtcgatggcg accggcacca acaccgccga cctcgacttc
     2461 gcctcggtgc agcgcggcaa ccccgagatc cagcgccgct gccaggaggt catcgacgcg
     2521 tgctggcagc agggcgagaa caatccgatc atcgccatcc acgacgtcgg tgccggcggc
     2581 ctgtcgaacg cgatgcccga gctcgccgat cacgccggcc tcggcgccca cttcgagctg
     2641 cgcgaggtgc acatcgaaga gccgggcatg agtccgcgcg agatctggtc gaacgaatcg
     2701 caggagcgct acgtgctcgc gatcgcgccc gagagcctgc cgatgttcca ggccttctgc
     2761 gagcgcgagc gctgcccctt cgcggtgctt ggcaccgcca ccgccgatgg ccacctcacc
     2821 gtgtccgatc gccacttcgg caacaagccg gtggacatgg acatgaaggt gctgctcggc
     2881 aagccgccga agatgacgcg caacgtctcg cggcgcgcgg tgcatctgcc gcctttcgac
     2941 accaccggct tcgatctcca ggaggctggg atgcgcgtgc tgcgcgtgcc cgcggtggcc
     3001 agcaagagct tcctgatcac catcggcgat cgctcggtcg gcggcctcac cgcgcgcgac
     3061 cagttcgtcg ggccttggca ggtgccggtg gccgacgtcg cggtcaccgc gatgagcttc
     3121 cagggctacc gcggagaagc cttcgcgatg ggcgagcgca cgccgctggc ctgcgtcgat
     3181 gcgccggcat cgggccgcat ggcgatcggt gaggcaatca ccaacatcgc cgccgccgac
     3241 atcgagaagc tcggcgacgt caagctctcc gccaactgga tggctgccgc cggccaccgc
     3301 ggcgaggacg cgcgtcttta cgacaccgta aaggccgtgt ccgagttctg cgtcagcgcc
     3361 ggcctgtcga tcccggtcgg caaggactcg ctgtcgatgc gcactgcctg gcgcgatggc
     3421 gaagaggaca agcaggtcgt cgctccgctg tcgctgatcg ccaccgcctt cgcccccgtg
     3481 caggacatcc gcagcacgct cacgccgcaa ctgcagctgc atgaaggcgt ggagaccgag
     3541 ctgctgctga tcgacctcgg caacggcaag aaccgcctcg gcggctcggc cttcgcccag
     3601 gcttacgagt cagtcggcga gcatgcgccg gatgtcgatc ccgcccagct cgcagcgttc
     3661 ttcgaaaccg tgcagcgact gcgccgcgac ggcctgctgc tggcctatca cgaccgttcc
     3721 gatggcggcc tcttcgccac cgtgtgcgag atggccttcg ccgcgaagtg cggcctgtcg
     3781 ctgatcctcg ataccgtttg ctacgacccc tacatgatgg acgtcgatgg tctggagaag
     3841 aagccggaca cgctcaaggg ccgtttcgac gaccgcctct tcgccggtct cttcgccgag
     3901 gaactcggcg cggtgatcca gatccgccgc gacgaccgct cccgcatcac cgaggtgctg
     3961 cgcgcggcca ggctcgctta ccacttcatc ggcgagccca acgacaagga tgagatccgc
     4021 ttccgccgca acgccaaact cgtgtttgcc gccagccggg tcgagctgct gcaggcgtgg
     4081 tcggagacca gctatcgcgt cgccaagctg cgcgacgatc ccgagtcggt gcagcaggaa
     4141 ttcgacgcgc tcgccgacgc cggcaacccc ggcctgtcgg tggcgctgtc cttcgacgtc
     4201 aaggaggatg tggcggcgcc cttcatcgcc agcactgcgc gtcccaaggt cgtcgtgctg
     4261 cgcgagcagg gtgtcaactc ccagttcgag atggcggcag ccttcgagcg tgccggcttc
     4321 accccggtcg acgtgcatat gtcggacctg caggccggtc gcatcgacct ggccgacttc
     4381 catggcctcg cagcttgcgg cggcttctcc tatggcgacg tgctcggcgc cgggcagggc
     4441 tgggccaagt ccatcctgtt caacccccgc ctgcgcgacg agttcgcggc tttcttcggg
     4501 cgcagcgaca ccttcgccct cggggtgtgc aacggctgtc agatgatggc ccacctcgcg
     4561 ccgatcatcc ccggcgccga agcctggccc accttccacc gcaaccgcag tgaacagttc
     4621 gaggcccgct tcgtgatggt ggaggtcgca gacagcccct ccatcctgct ccagggcatg
     4681 gccggcagcc gcatgccgat cgtggtcagc catggcgagg gcagggcggt gttccacagc
     4741 gaggccgacc gcgagcgcgc cctcctggcg ctgcgctacg tggacaacca cggcaatccg
     4801 gcacagacct atccggccaa ccccaacggc tcgccggagg gtgtgacggg cttcaccact
     4861 gccgacgggc gcttcacgat catgatgccg caccccgagc gcaccgcgcg gaccctgcag
     4921 atgtcgtggg cgccgcagtg gctggtcacg gacagtcccg acgcctcgcc ctggctgcgc
     4981 atgttccgca atgcccgctt gtggctcggc tgattgcagg gaccacgcga tccgggtaac
     5041 gcgatgtgag tttccgctca agtcgccgaa gcgtcagccg ttaagaggtt ccggctggtc
     5101 acggtggcgg acagcattgc gggggaatgg attgaaccag acgatttcga cgcgcgaatt
     5161 gaagatcggc atgttcgtcg tcgctatcga tcgtccatgg accgagacac cattcatgat
     5221 gcagggcttc ctgctggaga atgtcgacca gctccgggcc ctgcagcact actgccgttc
     5281 ggtcacgatc gatcgcacac gctcgatcgg cgaccagtac agcgccaagg tcttcgacaa
     5341 ggacgcgccc ctgcgtggcg cggacgggtt cgtgcgcaag caccccgacg tcaccgaatc
     5401 gcgtgtcggg gcgcagcgct tcatcaaggt cgcgcgcggc ttcaaaggca agatccacac
     5461 cctccgtcag ccgcacgtcc cgcgcatccg catcgaggac gggcgcagcc ggctcgagtc
     5521 cgagctgctc tactcggcac cgatcgtcga tgacgtgttc cgggccttgc gcgagaccca
     5581 gctcgcgatc gacagcaatg tcgccatcga cgtcccccag atcagcggcc tggtcggcga
     5641 gatggccgcc ggcgtgcagc gcaaccccga cgcactgctc tggctcaccc gcctcaagcg
     5701 caccgaccag tacgcctacg accacgcggt gaacgtctcc gtgcatgcga tggtgttcgc
     5761 gcgcttcttg ggcctgagcg gcaagcaggt gcatcagctc ggcctcgccg gcctgctgca
     5821 ggatatcggc aaggtgcaga tcgactacgg tctgctcagc aagcccgggc cgctcgacga
     5881 gcgtgagttc gagcacgtca aggcccatgt cacccacacg gtgcgcctgc tgcaggcgca
     5941 ccgggacttc gacgccgagg tgctggcgac catcgctgcg caccacgagc gcttcgacgg
     6001 caccggctac ccgcgcgggc tcgaaggcat gagcatcccg ctgccggcgg agatggccgg
     6061 catcaccgac gcctactgcg cgatgacccg cgaccgcgtc tatcgcgagg ccgtctccag
     6121 ccagcgcgcc atggagacgc tgaaccagct gcgcaacacg cagttccgcg acaccttggt
     6181 cgaccagttc ctgcagtgca tcggcctcta tccgatcggc accctggtcg a
//
LOCUS       DAOQNN010000175         6202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7171_length_6202_cov_9.562551, whole genome shotgun sequence.
ACCESSION   DAOQNN010000175 DAOQNN010000000
VERSION     DAOQNN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7171_length_6202_cov_9.562551"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /gene="torC"
                     /locus_tag="PKV97_10135"
     CDS             <1..1170
                     /gene="torC"
                     /locus_tag="PKV97_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HNB06276.1"
                     /translation="IGLGVLVTGGFIAGVLAWQGFNTAMEATNKEEFCLSCHTMHDNL
                     LPELQKTVHWNNRSGVRARCPDCHVPHDFTDKVARKMQASREVLGQLLGTIDTREKFK
                     EHRIVLAQREWKRFAANGSKECRACHDYKDMNFDKMRPASQVAMRKAAERNQSCVDCH
                     KGVAHQLPEMKGARNPAFDTLVSSASGQSVATGKDYFLVVPQELFADEGLSKRIGSVE
                     VATQVKVLETKGDAQRVEFALWRKNKGYGRVWYSHFGLNITSATFDKDVAQDTSFVKV
                     LDTREDPMTGLEWQQVTATAWMRKGSVLDSVEPVWSIARASYSSSCSVCHRQPDEAHF
                     DANTWPGLFGGMVGFTSMDDDTARVVLKYLQTHSSDFSKSHGAGSPDAALQTARP"
     gene            1187..3703
                     /gene="torA"
                     /locus_tag="PKV97_10140"
     CDS             1187..3703
                     /gene="torA"
                     /locus_tag="PKV97_10140"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HNB06277.1"
                     /translation="MNYSRRGFLKAMAAMSGSALVAPGLLTAAPALAAGEAAAVGDDI
                     TTWKISGSHFGAIRAKVVGDRVVDIRPFEHDKHPTEMVKGLLGLIYSPSRVRYPMVRL
                     DWYRKRHLSDTSQRGDNRFVRVSWDEALDLFYEELERIQKNYGPWALHTGGVGWRSVG
                     QVHSCGNHMVRGVGMHGRSVGTIGDYSTGAGQMILPYVLGSTEVYSQGTSWDVILKES
                     KLVVFWANDPIKNLQVGWNTETHESYAYFEQLKAKVADKSIQVICIDPVKSKTLNYLG
                     AAEHQYINPMTDVALMLAIAHTLVKENLHDQKFLDTYTLGFDQFLPYLQGKTEDKVEK
                     TPEWAEKICGVPSDRIRELARLMARHRTQLIFGWAVQRQQHGEQPYWMGAVLAAMLGQ
                     IGLPGGGISYAHHYSSVGVSSSGAAMPGAFPLNLDPGRKPRHDNTDYKGYSAVVPIAR
                     VIDALLEPGKEIDFNGGKVKLPPYKMAIFSGCNQWHRQPQRNRMKEAWQKLETVVAID
                     YNWTATCRFADIVLPACTPFERNDLDGYGSYSNRGILAMQKLIDPLFHSRPDFEIFRG
                     LTRRFNRDAEYTRGMDEMQWIEKIYEDCRKENGLKDIAMPPFAEFWQKGYVLFPEGKP
                     WVRHADFRDDAEVNALGTPSGFIEIFSRKIERYGYADCKGHPVWMEKTERSHGGPGSD
                     RFPLWLQSVHPDKRLHSQLCESEPLRETYAIAGREPVFIGPEDAAARGIAHGDLVRVF
                     NDRGQLLAGAHVSDNFPRGVVRIQEGAWYGPTGPEIGALDTYGDPNTLTLDIGTSSLA
                     QATSANTCLVQIEKFVGTPPRVTSFGGPIEVDPEGREVKA"
     gene            3703..4416
                     /gene="torD"
                     /locus_tag="PKV97_10145"
     CDS             3703..4416
                     /gene="torD"
                     /locus_tag="PKV97_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HNB06278.1"
                     /translation="MDARSQAGTPPPLSPAEWRFASTQRAGLYGWFSRLYAQEVTDEL
                     FRSHFADGGFAPFAGLAELGLAAEVQRLDDAVAALRAEQLPRLELAADFAQLFLLDGT
                     TSALPYASAYAGKDAASPLYGAAEARMREFLTARKLAIQADFREPADHLAVALAVMAE
                     VAEKDSGASDLPTAAATQADFLRTALLDWLPHFVARCQQARPRFDVYPALAALLLGFV
                     RADMAFLGDVGGGAGAAAD"
     gene            complement(4479..4799)
                     /locus_tag="PKV97_10150"
     CDS             complement(4479..4799)
                     /locus_tag="PKV97_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNB06279.1"
                     /translation="MALTRDFKQTVAARVQRDPAFAQALLDEAITLFINGEPDTAKLV
                     LRDLVNATLGFESLAEEIHKPAKSLHRMLSASGNPTMSNISAIFAAIKRALGVEVRTT
                     VVVA"
     gene            complement(4802..5140)
                     /locus_tag="PKV97_10155"
     CDS             complement(4802..5140)
                     /locus_tag="PKV97_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNB06280.1"
                     /translation="MPYHLRELVLPDGSSPYADWFSTLDPIAAAKVTVAAARMELGNL
                     SNVEWFRGIGEYKIDWGPGYRIYLEKDGLEIVVLLGGGSKKRQQRDIDEAVALWEDYK
                     RRKARMNKGA"
     gene            5298..5453
                     /locus_tag="PKV97_10160"
     CDS             5298..5453
                     /locus_tag="PKV97_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB06281.1"
                     /translation="MAGLFPLALLIRVIQMLLMGAYAHSPLRAVLVGSKTTDLLRAAT
                     IPTLLLR"
     gene            complement(5457..5654)
                     /locus_tag="PKV97_10165"
     CDS             complement(5457..5654)
                     /locus_tag="PKV97_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNB06282.1"
                     /translation="MKMMNCKDATRLMSERYERTLSLRERLALKVHTAMCRGCSNYGL
                     HLDVLRLAARRLREGCEGGER"
     gene            complement(5651..>6202)
                     /locus_tag="PKV97_10170"
     CDS             complement(5651..>6202)
                     /locus_tag="PKV97_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB06283.1"
                     /translation="IRRDMLRFARMQLRDSGAAEDMVQEALMAAMRSAKSFAGRSAFK
                     TWMFAILRNKIVDHIRASSREICSADLAGASELDAGLDEFDVLFKQGGHWNPDDRPAT
                     WADPEAAFEQDAFWTVFDLCMNKLPEHIARVYTMRELLELETAEICAALGITPNNCFV
                     ILHRARLGLRECLENRWFQGESR"
BASE COUNT         1075 a         2175 c         1984 g          968 t
ORIGIN      
        1 atcggcctcg gcgtgctggt caccggcggc ttcatcgccg gcgtgctggc ctggcaaggc
       61 ttcaacaccg ccatggaagc caccaacaag gaagagttct gcctgagctg ccacaccatg
      121 cacgacaacc tgctgcccga gctgcagaag acggtgcact ggaacaaccg cagcggcgtg
      181 cgcgcgcgct gcccggactg ccacgtgccg cacgacttca ccgacaaggt ggcgcgcaag
      241 atgcaggcca gccgcgaggt gctcggccag ctcctcggca ccatcgacac ccgcgagaag
      301 ttcaaggagc accgcatcgt gctggcgcag cgcgagtgga agcgcttcgc ggccaacggc
      361 tcgaaggaat gccgtgcctg ccacgactac aaggacatga acttcgacaa gatgcgtccg
      421 gcatcgcagg tggcgatgcg caaggccgcc gagcgcaacc agagctgcgt cgactgccac
      481 aagggcgtcg cccaccagtt gcccgagatg aagggcgcgc gcaacccggc cttcgacacc
      541 ctggtgtcct ccgcctccgg acagagcgtg gctacgggca aggactactt cctggtggtg
      601 ccgcaggaac tcttcgccga cgaaggtctg agcaagcgca tcggctcggt cgaggtcgcc
      661 acccaggtca aggtgctcga gaccaagggc gacgcgcagc gcgtcgagtt cgcgctgtgg
      721 cgcaagaaca agggctatgg tcgcgtctgg tacagccact tcggcctcaa catcaccagc
      781 gccaccttcg acaaggacgt cgcgcaggac acgagcttcg tgaaggtgct cgacacccgg
      841 gaagacccga tgaccggcct cgaatggcag caggtcaccg ccaccgcgtg gatgcgcaag
      901 ggtagcgtgc tcgacagcgt cgaaccggtg tggtcgatcg cccgcgccag ctacagcagc
      961 agctgcagcg tgtgccaccg ccagcccgac gaagcgcact tcgacgccaa cacctggccg
     1021 ggcctgttcg gcggcatggt cggcttcacc agcatggacg acgacaccgc ccgcgtggtg
     1081 ctcaagtacc tccagaccca ttcgtccgat ttcagcaagt cgcatggcgc cggttcgccc
     1141 gatgccgccc tgcagaccgc gcgtccctga tcaggagttg aacaccatga actactctcg
     1201 gcgaggtttc ctcaaggcca tggcggcgat gtcgggcagc gcgctggttg cgcccggtct
     1261 gctcaccgcc gccccggccc tcgcggccgg cgaggcggcc gcggtcggcg acgacatcac
     1321 cacctggaag atctccggct cgcacttcgg cgccatccgc gccaaggtgg tcggcgaccg
     1381 cgtggtcgat atccgcccct tcgagcacga caagcatccg acggagatgg tcaagggcct
     1441 gctcggcctg atctacagcc cgtcccgcgt gcgctacccg atggtgcgcc tggactggta
     1501 ccgcaagcgc cacctcagcg acaccagcca gcgcggcgac aaccgcttcg tgcgcgtgag
     1561 ctgggacgag gcgctcgacc tgttctacga agaactcgag cgcatccaga aaaactacgg
     1621 cccgtgggcg ctgcacaccg gcggcgtggg ctggcgctcg gtcggccagg tgcatagctg
     1681 cggcaaccac atggtgcgcg gcgtcggcat gcacggccgc agcgtcggta ccatcggcga
     1741 ctactcgacc ggcgccgggc agatgatcct gccctacgtg ctcggctcga ccgaggtgta
     1801 ctcgcaaggt acctcgtggg acgtgatcct caaggagagc aagctcgtcg tgttctgggc
     1861 caacgacccg atcaagaacc tgcaggtggg ctggaacacc gagacccacg agtcctacgc
     1921 ctacttcgag cagctcaagg ccaaggttgc cgacaagtcg atccaggtga tctgcatcga
     1981 tccggtcaag agcaagacgc tgaactacct cggcgctgcc gagcaccagt acatcaaccc
     2041 gatgaccgac gtggcgctga tgctcgccat cgcgcacacg ctggtgaagg aaaacctgca
     2101 cgaccagaag ttcctcgaca cctacaccct cggcttcgac cagttcctgc cctacctcca
     2161 aggcaagacc gaggacaagg tcgagaagac gcccgagtgg gcggagaaga tttgcggcgt
     2221 gccttctgat cgcatccgcg agctcgcccg cctgatggcc aggcaccgca cccagctcat
     2281 cttcggctgg gcggtgcagc gccagcagca cggcgagcag ccctactgga tgggcgcggt
     2341 gctcgccgcc atgctcggcc agatcggcct gcccggcggc ggcatcagct acgcgcacca
     2401 ctacagctcg gtgggcgtgt cctcgtcggg cgccgccatg cccggcgcct tcccgctcaa
     2461 cctcgacccc ggccgcaagc ccaggcacga caacaccgac tacaagggct acagcgcggt
     2521 ggtgccgatc gctcgcgtca tcgacgccct gctcgagccg ggcaaggaga tcgacttcaa
     2581 cggcggcaag gtcaagctgc cgccctacaa gatggcgatc ttcagcggct gcaaccagtg
     2641 gcatcgccag ccccagcgca accgcatgaa ggaagcctgg cagaagctcg agaccgtggt
     2701 cgccatcgac tacaactgga ccgccacctg ccgcttcgcc gacatcgtgc tgccggcgtg
     2761 cacgcccttc gagcgcaacg acctcgacgg ctacggctcg tacagcaacc gcggcatcct
     2821 cgcgatgcaa aagctcatcg acccgctctt ccactcgcgc cctgacttcg agatcttccg
     2881 cggcctcacc cggcgcttca accgcgacgc cgaatacacc cgcggcatgg acgagatgca
     2941 gtggatcgag aagatctacg aggactgccg caaggagaac ggcctcaagg acatcgccat
     3001 gccgcccttc gccgagttct ggcagaaggg ttacgtgctg ttcccggagg gcaagccctg
     3061 ggtgcgccac gccgacttcc gcgacgacgc cgaggtcaac gcgctcggca cgccgtcggg
     3121 cttcatcgag atcttcagcc gcaagatcga gcgctatggt tacgccgact gcaagggcca
     3181 cccggtgtgg atggagaaga ccgagcgctc gcacggcggc cccggctccg accgcttccc
     3241 gctgtggctg cagtcggtgc acccggacaa gcgcctgcac tcgcagctgt gcgaatccga
     3301 gccgctgcgc gagacctacg ccatcgccgg ccgcgagccg gtgttcatcg gccccgagga
     3361 cgccgccgcg cgcggcatcg cccacggcga cctggtgcgc gtcttcaacg accgcggcca
     3421 gttgctggcc ggcgcgcacg tgtcggacaa cttcccgcgc ggcgtggtgc gcatccagga
     3481 aggcgcctgg tacggaccga ccggtccgga gatcggcgcg ctcgacacct acggcgaccc
     3541 caacacgctg acgctcgaca tcggcacctc cagcctggcg caggccacca gcgccaacac
     3601 ctgcctggtg cagatcgaga agttcgtcgg caccccgccg cgggtgacgt ccttcggcgg
     3661 cccgatcgag gtcgatccgg aaggtcggga ggtgaaggcg tgatggacgc gcgatcgcag
     3721 gcaggaacac ccccgcccct ctcgcccgcg gaatggcggt tcgcgagcac gcagcgcgcc
     3781 gggctgtacg gctggttctc ccggctgtac gcgcaggagg tcaccgacga gctgttccgc
     3841 agccacttcg cggacggggg cttcgccccc ttcgcggggc tggccgagtt gggcctcgcc
     3901 gccgaggtgc agcgcctcga cgacgccgtc gccgcgctgc gcgcggagca actgccacgc
     3961 ctggagctgg ccgcggactt cgcccagctc ttcctgctcg acggcacgac cagcgccctg
     4021 ccctacgcct cggcctacgc gggcaaggac gccgcctcgc cgctctacgg cgcggcggag
     4081 gcgcgcatgc gcgagttcct caccgcgcgc aagctggcga tccaggccga cttccgcgaa
     4141 cccgccgacc acctcgccgt ggcgctggcg gtgatggcgg aggtggcgga gaaggactcg
     4201 ggggccagcg acctccccac cgcagccgcg acccaggcgg atttcctgcg caccgcgctg
     4261 ctggactggc tgccgcactt cgtcgcccgc tgccagcagg cgcggccgcg cttcgacgtc
     4321 tacccggcgc tggcggccct gctgctcggg ttcgtgcgtg cggacatggc gtttctgggg
     4381 gatgtgggcg ggggtgcggg ggcagccgca gactgacgcg cgcgccctgc accgtcacac
     4441 ccgacggtgc gagcgcacgt ctcggtgcgc gcccgcactc atgccacgac gacggtcgtc
     4501 cgcacttcga ccccgagcgc ccgcttgatg gcggcaaaga tcgccgagat gttgctcatc
     4561 gtcggattgc ccgacgccga cagcatccgg tgcaggcttt tcgcgggctt gtggatctcc
     4621 tcggcgaggg actcgaagcc aagggtcgcg ttcacaaggt ctcgcaaaac cagctttgcg
     4681 gtgtcgggct caccgttgat gaacagggtg atcgcctcgt cgagcagcgc ctgcgcgaac
     4741 gcggggtcgc gctgcacccg tgcagcgacg gtctgcttga agtcgcgggt cagtgccatg
     4801 gtcaggctcc tttattcatc cgggccttgc ggcgcttgta gtcctcccac aaggccaccg
     4861 cttcgtcgat atcgcgctgc tggcgcttct tgctcccgcc accgagcagc acgacgatct
     4921 cgagcccatc cttctccagg tagatgcggt atccagggcc ccaatcgatc ttgtactcac
     4981 cgatgccccg aaaccactcg acgttcgaga ggttgcccag ctccattctt gccgctgcta
     5041 ccgtaacctt cgcggccgcg atggggtcga gcgttgaaaa ccagtcggcg tagggactgc
     5101 tgccatcagg taaaacaagc tctctgagat ggtagggcat tgcttgccaa tggtaacaga
     5161 taagttactt ttaaggagag cgacttcagc gagcgttctc gaagattttg cacagcaggg
     5221 tcgggctgcc aacagaattg cttaatgcat atttcgatcg cattttacct gtggcagagg
     5281 ccggcgggaa aacgctgttg gcgggcctat tcccgctcgc gctgctgatc cgcgtgatcc
     5341 aaatgctgct gatgggcgcc tacgcccact cgccgctgcg cgcggtgctc gtcggcagca
     5401 agaccaccga cctgctgcgc gcagcgacga tcccgacgtt gctgctgcgc tgatgctcag
     5461 cgctcgccac cctcgcagcc ctcgcgcagg cgcctggcgg cgaggcgcag cacgtcgaga
     5521 tgcagcccgt agttcgagca gcccctgcac atcgccgtgt gcaccttcag cgccaggcgc
     5581 tcgcgcaggc tcagcgtgcg ctcgtagcgt tcggacatga gccgggtcgc gtccttgcag
     5641 ttcatcatct tcatcgggac tctccctgga accagcggtt ctccagacac tcccgcagcc
     5701 cgagccgcgc gcggtgcagg atcacgaagc agttgttggg ggtgatgccc agcgccgcgc
     5761 aaatctcggc ggtctcgagc tcaaggagct cgcgcatcgt gtagacgcgg gcgatgtgct
     5821 cgggaagctt gttcatgcag aggtcgaaca ccgtccagaa ggcgtcctgc tcgaaggcgg
     5881 cctcggggtc cgcccaggtc gcggggcgat cgtcggggtt ccagtgcccc ccctgcttga
     5941 acagcacgtc gaactcgtcc agcccggcgt ccagctccga ggccccggca agatccgcgc
     6001 tgcagatctc acgcgaggac gcacggatgt ggtcgacgat cttgttgcgc aggatcgcga
     6061 acatccaggt cttgaaggcg gaccgcccgg caaagctctt cgcgctccgc atcgccgcca
     6121 tcagcgcctc ctgcaccatg tcctcggccg cgccgctgtc gcgcagttgc atgcgggcaa
     6181 agcgcagcat gtcgcgccgg at
//
LOCUS       DAOQNN010000176         6106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7357_length_6106_cov_7.093042, whole genome shotgun sequence.
ACCESSION   DAOQNN010000176 DAOQNN010000000
VERSION     DAOQNN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7357_length_6106_cov_7.093042"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PKV97_10175"
     CDS             complement(<1..508)
                     /locus_tag="PKV97_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06284.1"
                     /translation="MQPRAFSLFVVGLTLVLVAAAWMLAWWLQPVYGDLTRLGGYAER
                     THGWNEPVLEFDPLPTSFGEWTRPVDILVVGDSFANLRPAQQWQNHLAAMTGWSVHTL
                     DVHRVDVDALRASTLFVEHPPKVVIWNVVERGLAADHAKVEGLCPSLRPAPPLALPPP
                     MAVVAHARA"
     gene            complement(508..2064)
                     /locus_tag="PKV97_10180"
     CDS             complement(508..2064)
                     /locus_tag="PKV97_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNB06285.1"
                     /translation="MLFNSPQFIYLYLPIVLVGFFWLAATSQRLAAAWLAAASCFFYG
                     WWNPVYLGLLGASIVFNYGMGIRLARESARPPARRRRALLVFAVGANLLLLGYFKYFN
                     FFADSARVVFDGHWHFDAIVLPLGISFFTFTQIAFLVDAWRGEAREFNFVHYTLFVTY
                     FPHLIAGPVLHHREMMPQFAHASTYRLDWENIAVGLAIFGLGIFKKVVLADGIAPHAN
                     AVFQAAEAGQALSLLDAWGGALAYTFQLYFDFSGYSDMAIGLSRMFGVKLPLNFDSPY
                     KAGSISEFWRRWHMTLSRFLRDYLYIALGGNRKGSLRRHANLFVTMVLGGLWHGAGWT
                     FVVWGALHGFYLMVNHAWRWLVGRLGLRGGRAWRVLGWALTFVAVVVAWVFFRANSFD
                     AALAMLGAMTGANGVAVPPDWLNALHWRLNRLAGEMGVGAGLLAGQFADAWVWRDLAI
                     LAAIAFFAPNTQQIMSRYRPALDFRGGVDEAGLLRFRIGTPWLVATVLLLATALTRGD
                     AISEFLYFQF"
     gene            complement(2068..3213)
                     /locus_tag="PKV97_10185"
     CDS             complement(2068..3213)
                     /locus_tag="PKV97_10185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB06286.1"
                     /translation="MRNRYLVLTELFLPTKGGTAVWFDEVYRRLGGKEIHILTAQVPG
                     AAEYDRDHPNSVHRLSLQRVGWLRPESLGMYLRFLVCALRLALTRRFDAIHAGRALPE
                     GLVAWLVARLSLRPVVIYAHGEELTGWGRGNKYKAMCFALRHADRVIANSDFTRDTLV
                     GMGVRPERIVLIHPGVDNERFRPGLPAGDLRARLGLGDSARLILSVGRLSRRKGFDTL
                     IRSLPALRARGVDVHLAIVGIGEDADYLDGVAREAGVAERVHRLGHVPMEDLPRWYNA
                     CDVFVLANREIGGDTEGFGMVFLEAAACGKAAIAGRAGGTGSAVLDGVTGLRVDASTG
                     AELVDALARLLDDPPLLESMGQAGLARVRQSLSWQAVAERTRNCLRA"
     gene            complement(3248..4369)
                     /locus_tag="PKV97_10190"
     CDS             complement(3248..4369)
                     /locus_tag="PKV97_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06287.1"
                     /translation="MLIVESDDWGPGPVADADALAGLASRLAAVRDQRGRPAVMTLGV
                     VCSLPDGAAMLADGLARYRRRALDAPEFAPMVEAMRGGCGAGVFALQRHGMEHFWPDA
                     LLARARVDASLRDWLADPQARSEGLPSHLQSRWIDASVLPSRPLDPAAVQCAVDEEAR
                     LLECLFGAAPVVAVPNTFVWDDAVERAWAGSGVRCIVTPGRRIEGRDGQGALMPPTRV
                     IRNGERTAGGLMQLVRDDYFEPIRGHRAEQVWQAVAAKRRLGRPVLLETHRESFVGAA
                     AVARAANDELERALRGVLERHPDVRFLSTAELADELAAAATGLVTGSLPHRVAVFLAR
                     VEHEPALRRFLKYSGLRFALAPVFLIAGVMHGRCAARAR"
     gene            4861..>6106
                     /locus_tag="PKV97_10195"
     CDS             4861..>6106
                     /locus_tag="PKV97_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNB06288.1"
                     /translation="MAVLLGNPRWPDARLAMLAHSEGHAAALLDAWRNSGEAALETIQ
                     GSFTLVLVDSARDRILLAVDRCGAERLCFAAVGGTLVFSTSARAVARHPLVAATIDRQ
                     ALYEYLYCHMVPSPDTAFAGVEKLLPAQCASFSGGRLERRFYWQLPYAPEVDADEASL
                     RKRFLDLTEQAVARQYDEKRSTGAFLSGGTDSSTVSGMLGKVSGQPARTYSIGFRAEG
                     FDEIEYARITSRHFGTEAHEYYVTADDIVEAMPLIAAAYDEPFGNASAAPTYFCAKMA
                     RETGIEIVLAGDGGDEFFGGNERYAKQGVFERYHRTPAALRKLLEPLVMNFPLGDSIL
                     PVRKAQSYIRQARIPLPDRLETYNFLHRDPLQEIFEPEFLSAVDTERPLKLARDAYFR
                     SASTEVIERMMHLDHKITLADND"
BASE COUNT         1086 a         2155 c         2054 g          811 t
ORIGIN      
        1 ccgcgcgggc atgcgccacg accgccatcg gcggcggcag cgcgagcggc ggcgcggggc
       61 gcaagctcgg acacagcccc tccaccttcg cgtgatccgc agccaggccg cgctcgacca
      121 cgttccagat caccaccttc ggcggatgtt ccacgaacag ggtcgacgcg cgcaaggcgt
      181 ccacgtctac gcgatggacg tcgagcgtat gcacgctcca gccggtcatt gcggcgaggt
      241 ggttctgcca ttgctgggcc ggtcgcaggt tcgcgaacga atcgccgacc accaggatgt
      301 ccaccggccg cgtccattcg ccgaacgagg tcggaagcgg atcgaattcg agcaccggct
      361 cgttccagcc atgcgtgcgc tcggcgtacc cgccgaggcg ggtcaggtca ccatagaccg
      421 gctgcaacca ccacgccagc atccacgcgg ccgcgaccag gaccagggtg agtccgacga
      481 cgaagaggga aaaggcgcgc ggctgcatca gaactggaag tacaggaact cggaaatcgc
      541 atcgccccgc gtcagcgccg tggcgagcag cagcacggtc gcgaccagcc agggggtgcc
      601 gatgcggaaa cgcagcagcc ccgcttcatc gacgccgccg cggaagtcga gcgcgggccg
      661 gtagcgggac atgatctgct gcgtgttggg cgcgaagaag gcgatcgccg ccaggatggc
      721 gaggtcccgc cagacccagg catcggcgaa ctgcccggcc agcagtcccg cgcccacccc
      781 catctcgccg gcgaggcggt tcagccgcca gtgcagcgcg ttgagccagt cgggcggcac
      841 ggccacgcca ttcgcgcctg tcatcgcgcc gagcatggcg agcgcggcat cgaaggagtt
      901 cgcgcggaag aacacccacg cgacgaccac cgccacgaag gtgagcgccc agccgagcac
      961 ccgccacgcc cggccgccgc gcaggccgag gcggcccacc agccagcgcc aggcatgatt
     1021 caccatcagg tagaacccgt gcagcgcccc ccagaccacg aaggtccagc ccgcgccgtg
     1081 ccagagcccg cccagcacca tcgttacgaa caggttggcg tggcgccgca acgacccctt
     1141 gcggttgccg cccagcgcga tgtacaggta gtcgcgcagg aaacgggaca aggtcatgtg
     1201 ccagcgccgc cagaactcgg agatgctgcc cgccttgtag ggcgaatcga aattgagcgg
     1261 cagcttgacg ccgaacatgc gggacaggcc gatcgccatg tcggaatacc cggagaaatc
     1321 gaagtagagc tggaaggtgt aggccagcgc gccgccccag gcgtcgagca ggctgagcgc
     1381 ctgcccggcc tcggcggcct ggaacacggc gttggcgtgc ggcgcgatac cgtcggcgag
     1441 gaccaccttc ttgaagatgc cgagcccgaa gatcgccagc ccgaccgcga tgttctccca
     1501 gtccagccgg taggtggatg cgtgcgcgaa ctgcggcatc atctcgcggt gatgcagcac
     1561 cggcccggcg atcaggtggg ggaaataggt cacgaacaag gtgtagtgga cgaagttgaa
     1621 ctccctcgcc tcgccccgcc aggcatcgac caggaaggcg atctgggtga aggtgaagaa
     1681 ggagatcccc agcggaagca cgatggcgtc gaaatgccag tgcccgtcga acacgacgcg
     1741 ggcgctgtcg gcgaagaagt tgaagtactt gaagtagccg agcaggagca ggttggcgcc
     1801 cacggcgaag acgaggagcg cgcgccggcg ccgggcgggc ggacgagcgg attcgcgcgc
     1861 caggcggatg cccatgccgt agttgaagac gatcgaggcg ccgagcaggc cgaggtagac
     1921 cgggttccac cagccgtaga agaagcacga cgccgccgcc agccatgccg cggcgaggcg
     1981 ctggctcgtg gcggcgagcc agaagaagcc gaccagcacg atcggcaggt acaggtagat
     2041 gaactgcggc gaattgaaca gcatcgctca ggcgcgcagg cagttgcggg tgcgctcggc
     2101 caccgcctgc cagctcagcg actggcgcac gcgcgcgagc cccgcctgtc ccatcgactc
     2161 gagcagcggg gggtcgtcca gcaggcgcgc gagggcgtcc accagttcgg cccccgtgct
     2221 cgcgtcgacg cgcaggccgg tcaccccgtc gagcaccgcc gagccggtgc cgccggcacg
     2281 tccggcgatc gccgccttgc cgcaggccgc cgcctcgagg aacaccatgc cgaagccttc
     2341 ggtgtcgccg ccgatctcgc ggttggccag cacgaacaca tcgcaggcgt tgtaccagcg
     2401 tgggaggtcc tccatcggca cgtggccgag ccgatgcacc cgctcggcga cccccgcctc
     2461 ccgcgccacg ccgtcgaggt agtcggcgtc ctcgccgatg ccgacgatcg ccagatgcac
     2521 gtcgacgccg cgcgcgcgca gcgccggcag gctgcggatc agggtgtcga aacccttgcg
     2581 ccggctcaag cgtccgacgg acaggatcag ccgtgccgaa tcgcccagcc cgaggcgcgc
     2641 gcgcaggtcg cccgcgggca gcccggggcg gaagcgctcg ttgtcgacac ccggatggat
     2701 gagcacgatg cgctcgggcc ggacccccat cccgaccagg gtgtcgcggg tgaaatcgct
     2761 gttggcgatg acccggtccg cgtggcgcaa ggcgaagcac atcgccttgt acttgttgcc
     2821 gcggccccag ccggtgagct cttcgccatg ggcgtagatc acgaccgggc gcaagctcag
     2881 gcgggcgacg agccaagcca ccagcccctc gggcagggca cgcccggcgt ggatcgcatc
     2941 gaatcgccgc gtgagcgcga gccgcagcgc gcagacgagg aacctgaggt acatgccgag
     3001 cgactccggc cgcagccagc cgacgcgctg caggctcagg cggtgcacgc tgttggggtg
     3061 atcgcggtcg tactcggcgg cgcccggaac ctgcgccgtc aggatgtgga tctccttccc
     3121 gccgagtcgg cgatagacct cgtcgaacca gacggcggtg ccgcccttgg tgggcaggaa
     3181 aagctcggtg aggaccaggt agcggttccg catgggagat ctcgggatcg aagcgcgcca
     3241 gatcgtatca ccgtgcccgc gcggcgcagc ggccatgcat cacgcccgcg atcaggaaga
     3301 ccggcgcgag cgcgaaccgc aggccgctgt atttcaggaa gcgccgcagc gcgggctcgt
     3361 gctcgacccg ggccaagaag acggccaccc tgtgcggcaa ggaaccggtg acgagccccg
     3421 tggccgcagc ggccagttcg tcggcgagct cggcggtgga caggaagcgc acgtccggat
     3481 ggcgttcgag caccccgcgc aaggcccgct ccagctcgtc gttcgccgcg cgcgcgaccg
     3541 ccgcggcgcc cacgaagctc tcgcgatggg tctcgagcag gaccgggcgg ccgaggcgcc
     3601 gcttcgccgc cacggcctgc cagacctgtt ccgcccgatg gccgcggatc ggttcgaaat
     3661 agtcgtcgcg caccaactgc atcagtccgc cggcggtgcg ctctccattg cggatgacgc
     3721 gcgtcggcgg catcagtgcc ccctggccgt cacgcccctc gatgcgacgc cccggggtga
     3781 cgatgcagcg cacgccgctc cccgcccacg cacgctcgac cgcgtcgtcc cagacgaagg
     3841 tgttcggcac cgccaccacc ggcgccgcac cgaacaggca ctcaagcagc cgcgcctcct
     3901 catcgacggc acattgcacc gccgcgggat ccaggggccg gctgggcagc accgaggcgt
     3961 cgatccagcg gctctgcaaa tgcgacggca gcccttcgct gcgcgcctgc ggatcggcca
     4021 gccagtcgcg cagcgaggca tcaacgcggg ctcgcgcaag cagcgcatcg ggccagaaat
     4081 gttccatgcc gtgtcgctgg agggcgaaga cccccgcgcc gcagccgcca cgcatcgcct
     4141 cgaccatcgg cgcgaactcg ggcgcatcga gcgccctgcg gcggtagcgc gcaaggccgt
     4201 cggccagcat ggcggccccg tccggcaggc tgcacacgac gccgagggtc atcaccgccg
     4261 gcctcccgcg ctggtcgcgc accgcggcga gccgggaagc gagccctgca agcgcgtcgg
     4321 cgtccgccac cggcccgggg ccccagtcgt cgctctcgac gatcagcacg ggctgtgcga
     4381 gcacgggttc gttccacagg gctcgcagag cggcacggtg cacaacgagg aatgccagca
     4441 ccgccgctcc gccccacagc acgagagcaa gaattgaaaa cgacatcgta aagacgctct
     4501 acctcgcatg gatcgttggg gcggacccgc cagccagcac aggccgtcgc gaacctgaat
     4561 gcaacggccg tacgcgtttc cccgcccacc tgtgtactgc accgcacttg cgcgaagcct
     4621 actcggctac catagaccgc gatgtgatca attttactat tcaagacgat gacgacacga
     4681 cgagtactgt gcggctggat caacttggac gaacgtgtcg caagccagga cgcactcgct
     4741 gcgatgctcg ccgccgaggg gctcgcaccg gcacagatcc gcgtgcagac gtccgaggca
     4801 ggcgttttgg cctgcaccca tcccgcgggg agcagtgcgg tcgacctgcg ctcgggcagc
     4861 atggccgtgc tgctcgggaa cccgcgctgg cccgacgcgc ggcttgccat gctggctcat
     4921 agcgagggcc acgcagccgc gctgctcgat gcctggcgga acagcggcga agctgccctg
     4981 gaaacgatcc agggcagctt caccctggtc ctcgtcgaca gcgctcgcga ccggatcctg
     5041 ctcgcggtcg accgctgcgg agcggagcgc ctgtgcttcg ccgccgtggg cggcacgctg
     5101 gttttttcca ccagcgcgcg agcagtggcg cgccacccgc tcgtggcagc gacgatcgac
     5161 aggcaggcgc tttacgaata cctctattgc cacatggttc caagccccga tacggcgttc
     5221 gccggcgtcg agaagctgct tcccgcccaa tgcgcgagct tctccggcgg ccgcctcgag
     5281 cgccgcttct actggcagct tccttacgcg ccggaggtcg acgccgacga ggccagcctg
     5341 cgcaagcgat tcctcgacct cacagagcag gcggtcgccc gtcagtacga cgagaagcga
     5401 tcgaccggcg ccttcctgtc cggcggcacg gacagttcga cggtaagcgg gatgctcggg
     5461 aaggtcagcg ggcagccggc gcgcacctac tcgatcggct ttcgtgcgga aggcttcgac
     5521 gagatcgaat atgcccgcat cacctcgcgc cacttcggca ccgaggcgca cgagtattac
     5581 gtgaccgcag acgacatcgt cgaagccatg ccgctgattg ccgcggccta cgatgaaccg
     5641 ttcggcaatg cctcggccgc accgacctat ttctgcgcga agatggcacg cgagaccggt
     5701 atcgaaatcg tgcttgcggg cgacggcggc gacgagttct tcggtggcaa cgagcgctat
     5761 gccaagcagg gcgttttcga gcgttatcac cgtacgcccg cagcactgcg caagctgctc
     5821 gagcccctgg tgatgaattt tccgctcggc gattcgatcc ttccggtgcg caaggcgcag
     5881 agctacatcc ggcaggcgcg cataccgctg cccgaccgtc tggagaccta caatttcctg
     5941 catcgtgatc cgctgcagga gatcttcgag ccggaattcc tctccgcggt cgacaccgaa
     6001 cgcccgctga aactcgcacg cgacgcctat ttccgcagcg cctcgacgga agtcatcgag
     6061 cgcatgatgc acctcgacca caagatcacc ctcgccgaca acgacc
//
LOCUS       DAOQNN010000177         6027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7524_length_6027_cov_10.224883, whole genome shotgun sequence.
ACCESSION   DAOQNN010000177 DAOQNN010000000
VERSION     DAOQNN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7524_length_6027_cov_10.224883"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..975
                     /gene="argB"
                     /locus_tag="PKV97_10200"
     CDS             73..975
                     /gene="argB"
                     /locus_tag="PKV97_10200"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNB06289.1"
                     /translation="MTQPVHQQDVTPARKAEILAEALPYIKRFFDKTIVIKYGGNAMT
                     DPHLKDCFARDVVLLKLVGLNPVVVHGGGPQIENLLTRMGKKGEFIQGMRVTDAETME
                     VVEMVLGGQVNKEIVNLINKHGGKAVGLTGKDAGFIRAKKLLMQKHGAPEGDLIDVGQ
                     VGEITGIDPSLISFLDQGDFIPVIAPIGVGEEGETYNINADVVAGKLAEILKAEKLVL
                     LTNTPGVLDKAGNLLTGLTPRQIDDLVADGTLSGGMLPKIGSALDAARNGVKSVHIID
                     GRVEHCLLLEILTDHGVGTMIKSK"
     gene            complement(1256..1693)
                     /locus_tag="PKV97_10205"
     CDS             complement(1256..1693)
                     /locus_tag="PKV97_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNB06290.1"
                     /translation="MSNPLTRRTDPLDDFFRGFFVRPVDFGGQQLATPGTDAPQMRVD
                     VKETAQGYEVHAELPGMKKEDIHVHIDGPVVSISAERKQEKEIKDGEKVLRTERYFGK
                     ISRSFQLGQEIDEAAASAKFNDGVLELNLPKKAEVQAKRLNIE"
     gene            1854..2057
                     /locus_tag="PKV97_10210"
     CDS             1854..2057
                     /locus_tag="PKV97_10210"
                     /inference="COORDINATES: protein motif:HMM:NF025733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HNB06291.1"
                     /translation="MNETTNVCPRCGAAFTCGMRAGEAECWCAALPAAFAVPAEGAGE
                     CYCPRCLAELIEARRKVPREARG"
     gene            complement(2014..2379)
                     /locus_tag="PKV97_10215"
     CDS             complement(2014..2379)
                     /locus_tag="PKV97_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNB06292.1"
                     /translation="MSHTSHAPAAQPTPPLRIDLAGCTDKAGLLARIATALRFPDWFG
                     HNWDALSDCLCDLSWLPAAAYRLEFLHADALRATAPETFATLCEILDEAGDFWRAEGV
                     GFGHTFIPALPEAPSGAPR"
     gene            complement(2391..2795)
                     /locus_tag="PKV97_10220"
     CDS             complement(2391..2795)
                     /locus_tag="PKV97_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HNB06293.1"
                     /translation="MLRTVLLRLLLLLVLGAGLPACSPGAAPDAPQAAQGLLQPSHAG
                     LPAEAIETLALIQRGGPFPYRKDGTTFQNRERLLPARPRGYYREYTVPTPGARDRGAR
                     RIVSGGDPPEVFYYTADHYRSFREIAPLRSLP"
     gene            complement(2802..4451)
                     /locus_tag="PKV97_10225"
     CDS             complement(2802..4451)
                     /locus_tag="PKV97_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB06294.1"
                     /translation="MLLPHGLQVLVVESQSSMRAQLRNMLASIGIDSPQFAVSASMAM
                     RRLRAQPFDLILSEYDLGEGQDGQHLLEDLRQHAIIPPDTVFVMITGERNYERVISAC
                     ELVPNDYILKPLTAESLRVRLLRAFEKRDAFLPVWQLMKLGDPVGALAACREARDRHP
                     QYLVDFMRLQAELHAQIGQIEEAAALYREILAARPIPWASLGLARMLVVREAWVEAEH
                     LLADLVARHPRFIEAHELLARVQEETGRPAEACGTLQRAADLSPYRLPRLRRLGELAL
                     QAGELDRAEHSLAEAVRRGSYSEFRDPEDHVRLVQAQLARERPDEALATLADLERNLV
                     RHPKGELCLAVGNALFEGHRNDAEGLRSAMVRALASPERIRELSPGLRRDLLELCYDR
                     KLESEGRQLLADLMRTHGDDRSLASTRAALKARGFENLSAEVEEQVQNEVRTLIGAGA
                     EKARSGDYDGAVAEMMNAARKMPGNPHVLFNAALALLRHIEHRGWNEAFAAQARSLIE
                     RTQRMAPANPRLAAITEFRHELMKRYGIRPERAPVTRTRRA"
     gene            complement(4597..>6027)
                     /gene="aspS"
                     /locus_tag="PKV97_10230"
     CDS             complement(4597..>6027)
                     /gene="aspS"
                     /locus_tag="PKV97_10230"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNB06295.1"
                     /translation="TNRVIDLRRPQMQKNLMLRYKTTMAFRRFLDGAGFIDVETPMLT
                     KSTPEGARDYLVPSRVHPGQFFALPQSPQLFKQLLMVAGYDRYYQIVKCFRDEDLRAD
                     RQPEFTQVDLETSFMNEHEITALVEEMIRYVFKEAMAVELPAPFPRMTYAEAMRRYGS
                     DKPDLRVTLELTDVTDAVQDVAFKVFSGPATSGGRVAAMRVPGGNSLTRGEIDEYTKF
                     VAIYGARGLAYIKVNDVTQPNEHGLQSPIVKNLHETALRTILERTGAQSGDLIFFGAD
                     KTKVVNDAMGALRIKLGHEKGYVTGEAWRPLWVVDFPMFEYDEDDKRWTACHHPFTSP
                     KDEHIDLLESNPGECLAKAYDLALNGWEIGGGSVRIHRADVQARVFEALNIGPEEQQA
                     KFGFLLDALKYGAPPHGGLAFGLDRIVTMMTGAESIRDVIAFPKTQRAQCLLTDAPSG
                     VDEKQLRELHIRLRQKVETQVEVERG"
BASE COUNT          878 a         1907 c         2269 g          973 t
ORIGIN      
        1 ctttgctgcg gtgcacttcc ggttccgcgg gctggccact agaatcccct tgtccgttca
       61 aggagtctcc cgatgaccca gcccgttcac cagcaagacg tcacccccgc ccgcaaggcc
      121 gagatcctcg ccgaggcgct gccctacatc aaacgcttct tcgacaagac catcgtcatc
      181 aagtacggcg gcaacgcgat gaccgatccg cacctcaagg attgcttcgc acgcgacgtg
      241 gtgctgctca agctggtcgg cctcaacccg gtggtggtgc acggcggcgg cccgcagatc
      301 gagaatctgc tgacccgcat gggcaagaag ggcgagttca tccagggcat gcgcgtgacc
      361 gacgccgaga ccatggaggt ggtcgagatg gtgctcgggg gccaggtgaa caaggagatc
      421 gtcaacctga tcaacaagca cggcggcaag gccgtgggcc tcaccggcaa ggacgccggc
      481 ttcatccgcg ccaagaagct gctgatgcag aagcacggcg cgccggaggg cgatctgatc
      541 gacgtcggcc aggtgggcga gatcaccggt atcgatccca gcctgatctc cttcctcgac
      601 cagggcgact tcatccccgt gatcgcgccg atcggcgtgg gcgaggaggg cgagacctac
      661 aacatcaacg ccgacgtggt cgccggcaag ctcgccgaga tcctcaaggc cgagaagctg
      721 gtgctgctca ccaacacccc gggcgtgctg gacaaggcgg gcaacctgct gaccggcctg
      781 acgccgcgcc agatcgacga cctggtcgcc gacggcaccc tgtcgggggg catgttgccc
      841 aagatcggct cggcgctcga cgcggcgcgc aacggcgtga agtcggtgca catcatcgat
      901 ggccgcgtgg aacactgcct gttgctcgag atcctcaccg accacggcgt cggcacgatg
      961 atcaagagca agtagaaccc gcgcgtttgc cgcgccgccg tcgaggatgg cgtgcgacgc
     1021 ggctcgcgac ttgcgtcgct cctacgaaaa ccggcgaggc ctcgggggcg ctgcaggggg
     1081 atcgcgactt gcgtcgctcc tacgcgaacc ggcgaggctt cgggggggct gtaggagcga
     1141 cgcgagtcgc gatggctgcc gcgccgcact tgccgaactc ggtctctggc tgaagacccg
     1201 ctcgcgaatc gcgccactcc tgaatcccat ttgtcccgag ctgccgggca ggcgtctact
     1261 cgatgttgag ccgcttggcc tggacctcgg ccttcttggg caggttcagc tcgagcacgc
     1321 catcgttgaa cttcgcgctt gccgcagcct cgtcgatttc ctggccgagc tggaagctgc
     1381 gcgagatctt gccgaagtag cgttcggtgc gcagcacctt ctcgccgtcc ttgatctcct
     1441 tctcctgctt gcgctcggca ctgatcgaca ccaccgggcc gtcgatatgc acgtggatat
     1501 cctccttctt catgccgggc agttcggcat gcacttcgta gccttgcgcg gtctccttga
     1561 cgtcgacccg catctgcggc gcgtcggtgc ccggggtggc gagctgctgg ccgccgaagt
     1621 cgacggggcg cacgaagaag ccgcggaaga agtcgtccaa cgggtcggtg cgacgggtga
     1681 gagggttgct catgtcgtgt cctcctgggt ccgggttctt gccgggggat ccggcttcaa
     1741 gaccaatata ggcgcggagg aagcgttttc aaggggcgcg cgcgtaccct ctgcccgtcc
     1801 ggctgcgcta accttgcggc ggagttcgaa cgaaccaggg aggcgagacg ccgatgaacg
     1861 aaaccaccaa tgtctgtccg cgctgcggcg cggccttcac ctgcggcatg cgcgcaggcg
     1921 aggcggagtg ctggtgtgcg gcgctgccgg ccgcgtttgc ggtgccggcc gaaggtgcgg
     1981 gggagtgcta ttgcccgcgc tgcctcgccg agctcatcga ggcgcgccgg aaggtgcctc
     2041 gggaagcgcg gggatgaagg tgtggccgaa gcccacgccc tcggcgcgcc agaagtcgcc
     2101 ggcttcgtcg aggatctcgc acagcgtggc gaaggtctcg ggcgcggtcg cgcgcagcgc
     2161 gtcggcatgc aggaattcga gccggtaggc cgcggcgggc agccaggaga gatcgcacag
     2221 gcaatccgag agcgcgtccc agttgtggcc gaaccagtcc gggaagcgca gcgcggtcgc
     2281 gatgcgcgcg agcagtccgg ccttgtccgt gcagccggcg aggtcgatgc gcagcggggg
     2341 agtcggctgc gcggcggggg cgtgcgaggt gtggctcatg gacggacggc tcagggcagc
     2401 gaacgcagcg gtgcgatctc gcggaagctg cggtagtggt cggcagtgta gtagaacacc
     2461 tcgggtggat cgccgccgct gacgatgcgg cgcgcgccgc gatcgcgcgc gccgggcgtg
     2521 ggcacggtgt attcgcggta atagccgcgc ggccgcgccg gtagcaggcg ttcgcggttc
     2581 tggaaggtgg tgccgtcctt gcggtagggg aaggggccgc cgcgctggat gagcgccagc
     2641 gtctcgatcg cctcggccgg cagcccggcg tgcgagggct gcagcaggcc ctgtgcagct
     2701 tgcggggcat cgggcgcggc gccgggcgag caggcgggca ggcccgcgcc gagtacgagg
     2761 aggagcagca ggcggagcag gacggtacgc agcatcgggg ttcaggcgcg gcgggtgcgg
     2821 gtgacggggg cgcgctcggg gcggataccg tagcgcttca tgagctcgtg gcggaattcg
     2881 gtaatcgccg ccaggcgcgg gttggcgggc gccatgcgct gggtgcgctc gatcaggctg
     2941 cgcgcctgcg cggcgaaggc ctcgttccag ccgcggtgct cgatgtggcg cagcaaggcg
     3001 agcgcggcat tgaacagcac gtgcgggttg cccggcatct tgcgcgccgc gttcatcatc
     3061 tcggcgaccg cgccgtcgta gtcgccgctg cgcgcttttt cggcgccggc gccgatcaag
     3121 gtcctgacct cgttctgcac ctgctcctcg acctcggcgg agaggttctc gaagccgcgt
     3181 gccttgagcg cggcgcgcgt gctcgccagg ctgcggtcgt cgccgtgggt gcgcatcagg
     3241 tcggcgagga gctggcggcc ctcgctctcg agcttgcggt cgtagcacag ctcgagcagg
     3301 tcgcggcgca gcccggggga gagctcgcgg atgcgctcgg ggctcgcgag cgcgcgcacc
     3361 atggcgctgc gcaggccttc ggcgtcgttg cggtggccct cgaacagggc gttgccgacg
     3421 gcgaggcaga gttcgccctt ggggtggcgg acgaggttgc gctcgaggtc ggcaagcgtg
     3481 gccaaggctt cgtccgggcg ttcacgggcg agctgggcct gcaccaggcg gacgtgatcc
     3541 tcggggtcgc ggaattcgga gtagctgccg cggcgcaccg cctcggcgag actgtgctcg
     3601 gcacggtcga gctcgccggc ctgcagggcg agctcgccga gccggcgcag gcgcgggagg
     3661 cggtagggcg agaggtcggc cgcgcgctgc agggttccgc aggcctcggc ggggcggccg
     3721 gtctcctcct gcacgcgtgc gagcagctcg tgcgcctcga tgaagcgcgg gtggcgggcg
     3781 acgaggtcgg cgagcaggtg ctcggcctcg acccaggcct cgcgcaccac cagcatgcgt
     3841 gccagcccga gcgaggccca ggggatggga cgcgcggcga ggatctcgcg gtacagcgcc
     3901 gcggcctcct cgatctggcc gatctgggcg tgcagctccg cctgcaggcg catgaaatcg
     3961 accaggtact gcgggtggcg gtcgcgcgcc tcgcgacagg cggcgagcgc gccgacgggg
     4021 tcgccgagct tcatcagctg ccacaccggc aggaaggcgt cgcgcttctc gaaggcgcgc
     4081 agcaggcgca cgcgcaggct ctcggcggtg agcggcttga ggatgtagtc gttgggaacg
     4141 agctcgcagg cgctgatcac gcgctcgtag ttgcgctcgc cggtgatcat cacgaacacc
     4201 gtgtcgggcg ggatgatggc gtgctggcgc aggtcctcga gcaggtgctg gccgtcctgg
     4261 ccctcgccga ggtcgtattc ggacaggatg aggtcgaagg gctgcgcgcg caggcggcgc
     4321 atcgccatgc tcgccgagac cgcgaactgc ggcgagtcga tgccgatcga ggcgagcatg
     4381 ttgcgcagct gcgcgcgcat gctcgactgg gattcgacga cgaggacctg caggccgtgg
     4441 gggaggagca tcggggcgtc cgggatcggg gggccgcgcg gcgcgcgggc catccgtgaa
     4501 gattacggca tggcgccggc aggctgaagc ccctgcggcc gccttcgcgg cgatccggca
     4561 ggggcttcgg gatctccggc cggtgtgccg ccggactcag ccgcgctcga cttcgacctg
     4621 ggtctcgacc ttctggcgca ggcggatgtg caactcgcgc agctgcttct cgtccacgcc
     4681 gctcggcgcg tcggtgagca ggcactgggc gcgctgggtc ttggggaagg cgatgacgtc
     4741 gcggatggat tcggcgccgg tcatcatggt gacgatgcgg tcgaggccga aggccaggcc
     4801 gccatgcggg ggggcgccgt acttgagcgc gtcgagcagg aagccgaact tggcctgctg
     4861 ctcctcgggg ccgatgttca gtgcctcgaa cacgcgcgcc tgcacgtcgg cgcggtggat
     4921 acgcaccgag ccgccgccga tctcccagcc gttcaaggcg aggtcgtagg ccttggccag
     4981 gcactcgccc gggttggatt cgagcaggtc gatgtgctcg tccttggggc tggtgaaggg
     5041 gtggtggcag gcggtccagc gcttgtcgtc ctcgtcgtac tcgaacatcg ggaagtcgac
     5101 cacccacagc ggacgccagg cctcgccggt gacgtagccc ttctcgtggc cgagcttgat
     5161 gcgcagcgcg cccatggcgt cgttgaccac cttggtcttg tcggcgccga agaagatcag
     5221 gtcgcccgac tgcgcgccgg tgcgctcgag gatggtgcgc agcgcggtct cgtgcaggtt
     5281 cttgacgatc ggcgactgca ggccgtgctc gttgggctgg gtgacgtcgt tgaccttgat
     5341 gtaggcgagg ccgcgggcgc catagatggc gacgaacttg gtgtattcgt cgatctcgcc
     5401 gcgggtcagg ctgttgccgc ccggcacgcg catcgcggcg acgcggccgc ccgaggtggc
     5461 ggggccgctg aagaccttga aggcgacgtc ctgcaccgcg tcggtgacgt cggtgagttc
     5521 cagcgtcacg cgcaggtcgg gcttgtccga gccgtagcgg cgcatcgcct cggcgtaggt
     5581 catgcgcggg aagggcgcgg gcagctcgac cgccatcgct tccttgaaca cgtagcggat
     5641 catctcctcg accagggcgg tgatctcgtg ctcgttcatg aacgaggtct cgaggtcgac
     5701 ctgggtgaat tcgggctggc ggtcggcgcg caggtcctcg tcgcggaagc acttgacgat
     5761 ctggtagtag cggtcgtagc cggccaccat cagcagctgc ttgaagagct ggggcgactg
     5821 cggcaaggcg aagaactggc ccgggtgcac gcgcgagggc accaggtagt cgcgcgcgcc
     5881 ttcgggcgtg ctcttggtca gcatcggcgt ctcgacgtcg atgaagccgg cgccgtcgag
     5941 gaagcggcgg aaggccatcg tggtcttgta gcgcagcatc aggttcttct gcatctgcgg
     6001 gcgacgcagg tcgatgacgc ggttggt
//
LOCUS       DAOQNN010000178         6020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7537_length_6020_cov_9.125231, whole genome shotgun sequence.
ACCESSION   DAOQNN010000178 DAOQNN010000000
VERSION     DAOQNN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7537_length_6020_cov_9.125231"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..670)
                     /gene="scpB"
                     /locus_tag="PKV97_10235"
     CDS             complement(68..670)
                     /gene="scpB"
                     /locus_tag="PKV97_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNB06296.1"
                     /translation="MAPATTPETWLRIVEAALLSAPGALPVSELRKLFDEDPGPDLVR
                     RLLDELRAQWFEAGRGVELVQLASGWRFQTRPEYQVYLDRLKEEKPPRYSRAVMETLA
                     IIAYRQPVTRGDIEDIRGVAVSPNVLKTLESRGWVDIVGHRDTPGRPALFATTRRFLD
                     EMGLRSLTELPALTELEKIMDLVDVSQTEPAAAPAPEEEV"
     gene            complement(648..1478)
                     /locus_tag="PKV97_10240"
     CDS             complement(648..1478)
                     /locus_tag="PKV97_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNB06297.1"
                     /translation="MNLPLDLVPIDTGPPLDVLARIYGEPLLELPKDLYIPPDALQVF
                     LEAFEGPLDLLLYLIRKANLDILDVPMAPLTAQYLEYVEAMRATHLELAAEYLLMAAV
                     LLEIKSRMLLPRPPRASAEEEDPRAELVRRLLEYEQMKLAAARLEALPRVERDFEWVG
                     VFVAEKVVERLPEVSLHDLQLAWLRIMKKARLNQSHRVGREQLSVREHMTAILRKLGD
                     GAFVVFDALFDVELGPPGLVVSFLAVLELVKEKLVEVTQNEAFAPIYVKLADGTRNDA
                     "
     gene            complement(1516..2718)
                     /locus_tag="PKV97_10245"
     CDS             complement(1516..2718)
                     /locus_tag="PKV97_10245"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNB06298.1"
                     /translation="MYAERVLSGMRPSGRLHLGHYHGVLKNWVKLQEEFPCLFFVADW
                     HALTTHYDTPEVIENSVWDMLVDWLAAGVDPAQSTLFIQSQVPEHAELHLLLSMMTPL
                     GWLERVPTWKEQQERIEGRDLSTYGFLGYPLLQSADILIYRADKVPVGEDQVPHIEIT
                     REIARRFNHLYGREPGFEDKAKDAVRKLGAKRARMYHDLRTRFQQEGDDEALEQAKAL
                     LDETQNLSLGDRERLYGYLEGSGKMILVEPEALLTPAARMPGLDGQKMSKSYGNTVNL
                     REDAESVTRKVRTMQTDPARMRRTDPGEPERCPVWQLHAIYSGEDTLAWVQRGCRSAG
                     IGCLECKQPVLEAILKEQEVLHERAQPYLDDPSLLRSIIADGCERARRLAQETMRDVR
                     EAMGLDYG"
     gene            complement(2725..3384)
                     /locus_tag="PKV97_10250"
     CDS             complement(2725..3384)
                     /locus_tag="PKV97_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNB06299.1"
                     /translation="MDSLISTLAIWALPVVLAITLHEAAHGYVARHFGDPTAHLAGRI
                     TLNPLRHIDPVGTILVPAGILALSSLAGGGGILFGWAKPVPVNFSRLRNPKSDMLWVA
                     AAGPFVNFVMALGWALLFRIGATSEPGAYTLPVLEMADAGIRINAVLMALNLLPIPPL
                     DGGRIAVSLLPNRLAWQYARLEPYGFPILLVLLFTGVLGTLLWPLLTGFRYLLSLVFG
                     F"
     gene            complement(3412..4035)
                     /locus_tag="PKV97_10255"
     CDS             complement(3412..4035)
                     /locus_tag="PKV97_10255"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNB06300.1"
                     /translation="MAQYFSLHPEQPQARLIRQAAEILRGGGLVALPTDSAYALACQV
                     GDAALLDRIRRIRGVDERHHFTLMCRDLSEIGTYARVDNSQYRLLKAVTPGPYTFILE
                     GTKELPRRVLHPKRKTIGLRVPEHAVVSALLAEIDGPLLTSTLILPGEDLPLSDPEEI
                     RDRLGKLVELVIEAGYCGPEATTVIDLTSGAPELVRAGRGPLEPFGL"
     gene            complement(4063..4968)
                     /locus_tag="PKV97_10260"
     CDS             complement(4063..4968)
                     /locus_tag="PKV97_10260"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HNB06301.1"
                     /translation="MSVPSASRLHALNVDLHCHSTVSDGWLEPEALVLRAIANGVELL
                     ALTDHDELGGIASAQVVARANGLAFVPGVEISVSFAGETVHIVGLGVDPGSAELCAGL
                     AALRAGRDGRARRIGDELARVGIPDAFAGACRFARNPALVSRAHFARHLVASGVMPDV
                     KTVFDHYLVRGKPGFVEHRWATLEQAVGWIRAAGGIAVVAHPARYRLSSVAMGELFDR
                     FVAAGGEAVEVVAGAHTEDEMRRFASLARQRGLLASRASDFHGVQESMVDVGRCNPLP
                     ADLEPVWSRLQATTAALAAAASSAR"
     gene            complement(5392..>6020)
                     /locus_tag="PKV97_10265"
     CDS             complement(5392..>6020)
                     /locus_tag="PKV97_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HNB06302.1"
                     /translation="LRMMIMGIPNVGKSTLMNALLKRKVAKVGDEPAVTKHQQTLDLG
                     PGMTLTDTPGMMWPKIDHDADGYMLAASHAIGRNAVIDEEVAAFLGEILIARYPDRLA
                     VRYRLDAAAVAALDGPALVEAVGRRRGCLVKGGGLDLEKAAQILLQDYRDGALGRISL
                     ETPETRAHMIAAAQAAAAQAAAAGETGEEAAGAAEAGGEADASGGEGD"
BASE COUNT          939 a         2046 c         2155 g          880 t
ORIGIN      
        1 gggctgggcg tcgtcgtcgc gcgcgccacg ggattcgcgc gggtcgcggt cgaggatctc
       61 gatcgcgtca gacttcttct tcaggggcgg gcgcagcggc cggttcggtt tgcgagacat
      121 cgactagatc catgattttt tcgagttcgg tgagcgccgg cagctcggtc aggctgcgca
      181 gccccatctc gtcgaggaag cgccgggtgg tggcgaagag cgccggccgc ccgggcgtgt
      241 cgcggtggcc gacgatgtcg acccagccgc gcgattcgag ggttttcaag acgttgggcg
      301 agaccgccac gccgcggatg tcctcgatgt caccgcgagt gacgggctgg cgataggcga
      361 tgatcgccag cgtttccatg accgcgcgcg agtagcgcgg cggtttttct tccttcaggc
      421 ggtcgaggta gacctgatac tcgggccgtg tctggaagcg ccagccgctc gccaactgca
      481 ccagctcgac cccgcgcccg gcctcgaacc actgcgcgcg cagctcgtcg agcaggcggc
      541 gcaccaggtc cggccccgga tcctcgtcga agagcttgcg cagctccgac accggcagtg
      601 cccccggcgc ggagagcagc gccgcctcga cgatgcgcaa ccaggtctca ggcgtcgttg
      661 cgggtgccat ctgccagctt cacgtagatc ggcgcaaagg cctcgttctg ggtcacttcg
      721 accagctttt ccttcaccag ctcgagcacg gcgagaaagc tcaccaccag gccgggcggg
      781 ccgagctcga cgtcgaacaa ggcgtcgaac accacgaagg cgccgtcacc gagcttgcgc
      841 aggatcgccg tcatgtgttc gcgcaccgag agctgctcgc ggccgacacg gtggctctgg
      901 ttgagccgcg ccttcttcat gatacgcagc caggccagct gaaggtcgtg caggctgacc
      961 tcgggcagac gctcgaccac cttctcggcg acgaacaccc ccacccactc gaagtcgcgc
     1021 tccacccgcg gcagcgcctc gagccgtgct gcggcgagct tcatctgttc gtactcgagc
     1081 agccggcgca ccagctcggc gcgcggatcc tcttcttccg cactcgcgcg cggcggccgc
     1141 ggcagcagca tgcgcgactt gatctcgagc agcaccgcgg ccatcagcag gtattcggcg
     1201 gcgagctcga ggtgggtggc ccgcatcgcc tcgacgtact cgaggtactg cgcggtgagc
     1261 ggcgccatcg gcacatcgag gatgtcgagg ttggccttgc ggatcaggta gagcagcagg
     1321 tccagcggcc cctcgaaggc ttccaggaag acctgcaacg catcgggcgg aatatacagg
     1381 tctttcggca attcgagcag gggctcgccg tagatgcgcg cgagcacatc gagcggcggg
     1441 ccggtgtcga tcggcaccag gtcgagcggc aggttcatgg tgcggctgcg atcgggaagg
     1501 cggcgtgcgg cgggctcaac cgtagtccag acccatcgcc tcgcgcacgt cgcgcatggt
     1561 ctcctgcgcg agccggcgcg cgcgctcgca gccgtcggcg atgatgctgc gcagcagcga
     1621 aggatcgtcc agataaggct gggcgcgctc gtgcagcacc tcctgctcct tcaggatcgc
     1681 ctccagcaca ggctgcttgc actccaggca gccgatgccc gcgctgcggc aaccccgctg
     1741 cacccaggcc agggtgtctt cgccggaata gatcgcgtgc agctgccaca ccgggcagcg
     1801 ctcgggctcg cccgggtcgg tgcggcgcat gcgcgcggga tcggtctgca tggtgcgcac
     1861 cttgcgcgtg acgctctcgg cgtcctcgcg caggttgacg gtgttgccgt aggacttgga
     1921 catcttctgc ccgtccaggc cgggcatgcg cgccgccggg gtaagcagcg cctcgggctc
     1981 gaccaggatc atcttgccgc tgccttccag gtagccgtag aggcgctcgc ggtcgccaag
     2041 cgagagattc tgggtttcgt cgagcagggc cttggcctgc tcgagcgcct cgtcgtcgcc
     2101 ctcctgctgg aagcgcgtgc gcagatcgtg gtacatgcgc gcacgcttgg caccgagctt
     2161 gcgcaccgcg tccttggcct tgtcttcgaa gcccggctcg cggccgtaga ggtgattgaa
     2221 tcggcgtgcg atctcgcggg tgatctcgat gtgcggcacc tggtcctcgc ccaccggcac
     2281 cttgtccgca cggtagatca ggatgtcggc cgactgcagc agcggatagc cgaggaagcc
     2341 gtaggtcgac aggtcgcgac cctcgatcct ctcctgctgc tccttccagg tcggcacacg
     2401 ctccagccag ccgagcgggg tcatcatcga aagcagcagg tgcagctcgg cgtgctcggg
     2461 cacctgcgac tggatgaaga gcgtggactg cgcgggatcg accccggcgg cgagccagtc
     2521 gaccagcatg tcccagacgc tgttctcgat cacctcgggg gtgtcgtagt gcgtggtgag
     2581 cgcatgccag tcggcgacga agaacaggca ggggaactcc tcctgcagct tcacccagtt
     2641 cttgagcacg ccgtggtagt ggccgagatg gagccgccca ctggggcgca tgccggagag
     2701 gacacgttca gcgtacatgg agcatcagaa tccgaagacg agggacagca ggtagcggaa
     2761 cccggtgagc agcggccaca gcagggtgcc gagcacgccg gtgaacagca gcacgagcag
     2821 gatcgggaag ccgtagggct cgagccgggc atactgccag gccagccggt ttggcagcag
     2881 gctgaccgcg atgcggccgc cgtcgagcgg cgggatcggc agcaggttga gcgccatcag
     2941 cacggcgttg atccggatgc cggcgtcggc catctcgagc accggcagcg tgtaggcgcc
     3001 cggctcgctg gtggcgccga tgcggaacag cagcgcccac ccgagcgcca tgacgaagtt
     3061 gacgaaaggc cctgcagccg ccacccacaa catgtcggac ttgggattgc gcaggcgcga
     3121 gaagttgacc ggcaccggct tggcccagcc gaagaggatg cccccgccac cggcgagcga
     3181 gctgagggcg aggatgcccg ccggcaccag gatggtgccc accgggtcga tgtggcgcag
     3241 cgggttgagc gtgatgcgcc cggcgaggtg ggcggtgggg tcgccgaagt ggcgtgccac
     3301 gtaaccgtgc gcggcctcgt gcagcgtgat cgcgagcacg accgggaggg cccagatggc
     3361 cagggtggag atcagcgaat ccatcgcggg ctcggtggca aaccccggat tctacagacc
     3421 gaagggctcg agcgggccgc gcccggcgcg caccagctcg ggcgcgcccg aggtgaggtc
     3481 gatcacggtg gtggcctcgg gaccgcagta gccggcctcg atgaccagct cgaccagctt
     3541 gccgaggcgg tcgcgaatct cctccgggtc ggagagcggc aggtcctcgc cgggcaggat
     3601 cagggttgag gtgagcagcg ggccgtcgat ctcggcgagc agggccgaca ccaccgcgtg
     3661 ctcgggcacg cgcaggccga tggtcttgcg cttcgggtgc agcacgcggc gcggcagctc
     3721 cttggtgcct tcgaggatga aggtgtaggg gccgggagtg accgccttca gcaaccggta
     3781 ctggctgttg tccacccggg cgtaggtgcc gatctcggag aggtcgcggc acatcagcgt
     3841 gaagtggtgg cgctcatcca ccccgcggat gcggcggatg cggtcgagca gcgcggcatc
     3901 gcccacctgg caggcgagcg cgtaggcgga gtcggtgggc agcgcgacga ggccgccgcc
     3961 acgcaggatc tcggcggcct gacggatcag ccgcgcctgc ggctgttcgg gatgaaggga
     4021 gaaatactgg gccatgatcg gatgctcgat ggggcgcgcg ccctagcgcg cggaggatgc
     4081 ggccgccgcg agcgccgcgg tggtggcctg cagacggctc cacaccggct cgaggtcggc
     4141 aggcagcgga ttgcagcggc cgacgtcgac catgctctcc tgcacaccat ggaaatcgga
     4201 cgcgcgcgag gccagcagcc cgcgctggcg cgcgaggctc gcgaagcgac gcatctcgtc
     4261 ctcggtgtgc gcaccggcca ccacctcgac ggcctcaccg ccggcggcga cgaagcggtc
     4321 gaagagctcg cccatggcga cggaggacag ccggtagcgc gccggatgcg cgaccaccgc
     4381 gataccgccg gcggcgcgga tccagcccac cgcctgctcg agcgtcgccc agcggtgctc
     4441 gacgaagccg ggcttgccgc gcacgagata gtggtcgaag accgtcttca cgtcgggcat
     4501 cacgccgctc gcgaccagat ggcgggcgaa gtgcgcgcgg ctcacgagcg cggggttgcg
     4561 cgcgaagcgg caggcacccg caaaggcatc ggggatgccg acgcgcgcga gctcgtcgcc
     4621 aatgcgccgc gcgcgcccgt cgcgtccggc acgcagggcg gcgagaccgg cacacagttc
     4681 ggcgctcccg ggatcgacgc ccaggcccac gatatggacg gtctcgccgg cgaaggagac
     4741 cgagatctcg acgcccggca cgaaggccaa gccattggcg cgggcgacaa cctgcgcgga
     4801 cgcgatgccg ccaagttcgt cgtgatcggt cagcgcgagc aactcgacgc cgttggcgat
     4861 cgcgcgcaac accagcgctt cgggctcgag ccagccgtcc gagacggtgg aatggcagtg
     4921 caggtcgacg ttgagggcgt gcagccgcga ggctgacgga acgctcatgg gactccggag
     4981 gaggaacggc aggatcagga tgatatcaaa ggcgcccgga ctgcgccggc atgggccatg
     5041 cggcgacctg cggacgggca ggcgcgagca gccgtcgatc gtgcggccgc gtggcctgcg
     5101 acgcctgccg cacccgcgtc cgcccgccgt atcattgcac ccgaacaccc ggccccgatc
     5161 gatgagcttc gtcccccccg ccccacctgt cgccgaactg cgccgctgcc ccagctgcaa
     5221 tcgctggggc ggatggcggc gcgtgggcga ggacggcagc accgtcgaac tgcatcccac
     5281 cgagcagcgc ggcccctgcc gggaaggccc ctggcacggc tcgctgcgcg ggccgcgcaa
     5341 cgcctgtggg cagtggttgc gctggataga gatcgtgccg gcgacggcgg atcagtcgcc
     5401 ctcgcccccg gaagcatcgg cctcgccccc cgcttccgcc gcgccggcgg cttcctctcc
     5461 ggtctccccc gcagcggcag cctgagccgc ggccgcctgc gcggcggcga tcatgtgcgc
     5521 gcgcgtctcc ggcgtctcca ggctgatgcg cccgagcgcg ccgtcgcgat agtcctgcag
     5581 caggatctgc gccgccttct ccaggtccag cccaccgccc ttgaccagac agccgcgccg
     5641 ccggcccacc gcctcgacca gcgccgggcc gtcgagcgcg gccaccgcgg cggcgtcgag
     5701 ccggtagcgc accgccaggc gatcggggta gcgcgcgatg aggatctcgc cgaggaaggc
     5761 cgccacctcc tcgtcgatca ccgcgttgcg gccgatggcg tggctcgccg ccagcatgta
     5821 gccgtcggca tcgtggtcga tcttgggcca catcatgccg ggcgtgtcgg tgagcgtcat
     5881 gccgggaccg agatccagcg tctgctggtg cttggtgact gcaggctcgt cgcccacctt
     5941 ggccaccttg cgcttgagca gcgcgttcat cagcgtcgat ttgccgacgt tgggaatgcc
     6001 catgatcatc atgcgcagcg
//
LOCUS       DAOQNN010000179         6010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7572_length_6010_cov_10.930311, whole genome shotgun sequence.
ACCESSION   DAOQNN010000179 DAOQNN010000000
VERSION     DAOQNN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7572_length_6010_cov_10.930311"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /gene="groEL"
                     /locus_tag="PKV97_10270"
     CDS             <1..938
                     /gene="groEL"
                     /locus_tag="PKV97_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNB06303.1"
                     /translation="VAKSGRPLLIIAEDVEGEALATLVVNNIRGILKTCAVKAPGFGD
                     RRKAMLEDIAILTGGQVIAEEVGLTLEKATLDDLGQAARIEVGKENTIIIDGAGQADR
                     IEARVKQIRVQIEEASSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDA
                     LHATRAAVEEGIVPGGGVALLRARAALGELKGDNHDQDAGIKIVLRAMEQPLREIVAN
                     AGDEPSVVVNKVVEGSGNYGFNAATGEYGDMVEMGVLDPTKVTRTALQNAASVAGLML
                     TTDCMVGELAEDKPMGGMPDMGGMGGMGGMGGMGM"
     gene            1290..1982
                     /locus_tag="PKV97_10275"
     CDS             1290..1982
                     /locus_tag="PKV97_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB06304.1"
                     /translation="MRILLAEDDPVIADGICRALRRGGCAVDHVADGMEADAALAGQG
                     YDLLILDLGLPRMNGIEVLKRLRARKSAQPVLILTAQDGVDDRVRGLDAGADDYLTKP
                     FALPELEARVRALTRRGTGQPRCIEIGQLSYDQADRVVKIGGQLVELSAREIGLLEIF
                     LLRVGRLVSKDQLVDHLCGWGEEVSSNAIEVYVHRLRKKLEDSGVRIVTVRGLGYCLE
                     NPDAAPAAKVQA"
     gene            1951..3450
                     /locus_tag="PKV97_10280"
     CDS             1951..3450
                     /locus_tag="PKV97_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HNB06305.1"
                     /translation="MLPLRRKSRPERAPRGANAPRKSGPPNSLFGEILDWMLAPLLFL
                     WPISIIVTHNVADSIANQPYDLALADGVRALARLVEVEDGQVVVRFPAPPRALFRADQ
                     DDVVYYQVADVNGVTVSGDAEIPPMPTSSAVDGEEVQLRDAMIQGEEVRIAWRFVRPD
                     PAQADRFVLVQVAETRNKRTDLASRVVTGVLLPQFAIIPLAVVLVWVGLSRGIAPLNR
                     LQSLIRRRRPTDLSPVEPSSVPEEVRPLIIAYNDMMARLEENLQAQQRFIADAAHQMR
                     TPLTGLKMQTDLAMHETDPDQLRQSLTRIAESTDRAAHLINQLLSLARAEASFEKLYA
                     VETVDLDAIVREVALELYPRAQAKRIDLGVEAGDTALRVEGNPVLLREMLKNLVDNAI
                     KYTPRGGSVTARTRYAGSPVFEVEDNGPGIPEADRERVFERFYRVLGSGVDGSGLGLP
                     IVREIAELHRGTVTLDAGPGGRGTLARVVFPRSHLQPPRQVQGDHDALG"
     gene            3518..3593
                     /locus_tag="PKV97_10285"
     tRNA            3518..3593
                     /locus_tag="PKV97_10285"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3551..3553,aa:Phe,seq:gaa)
     gene            3733..4989
                     /gene="hemA"
                     /locus_tag="PKV97_10290"
     CDS             3733..4989
                     /gene="hemA"
                     /locus_tag="PKV97_10290"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNB06306.1"
                     /translation="MQLFALGLNHHTAPLVIRERVAFQPERMGQALHDLAHTDSVREA
                     AILSTCNRTELYFAAEQPQHAADWLARFHRLALAEVSPYLYSYPQRDAIRHVFRVASG
                     LDSMVIGEPQILGQVKDAARYAEEAGTLGVLLHKLFQNTFAVAKEVRSTTAIGANTVS
                     MAAAAVHLTERIFERVADQHVLFIGAGEMIELCAAHFGGARPKSMAVANRTEERARAL
                     ADRFGATTMRIDQIGEALPRFDVVVSCTAAPLPIVGLGMVERAVKARRHRPMVMVDLA
                     VPRDIEPEIAELDDIFLYTVDDLAQVVEAGMESRQQAVIEAEQIIASRVDGFLHWMSA
                     RETVPVIRSLREHAESLRQAELERAVRLLAKGEDPQRVLEALSHGLTNKLMHGPTRFL
                     NQAEGEHKVEAGRVVRELFNLSRDSH"
     gene            5047..>6010
                     /gene="prfA"
                     /locus_tag="PKV97_10295"
     CDS             5047..>6010
                     /gene="prfA"
                     /locus_tag="PKV97_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNB06307.1"
                     /translation="MKQSIRDKLEHLANRLEELDRTLASEEGARDMNAFRELSRERAE
                     IEPVVALYREHRQAEADCATARELLADPEMRELGELELAEGEARIEALEAELQRALLP
                     RDPNDARNLFLEIRAGTGGDEAALFAGDLLRMYTRYAERQRWKVEIVSASESDLGGYK
                     EVIARVVGNGAYSKLKFESGGHRVQRVPVTETQGRIHTSACTVAVLPEADEVEAVDIN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHIPTGIVVECQDDRSQHRNKAQAMAVLAA
                     RLYDARVRAQQSAEAAQRKSLVGSGDRSERIRTYNFPQGRVTDHR"
BASE COUNT          973 a         2127 c         2038 g          872 t
ORIGIN      
        1 aggtcgccaa gtcgggccgt ccgctgctga tcatcgccga ggacgtcgaa ggcgaggcgc
       61 tcgccaccct ggtggtgaac aacatccgcg gcatcctcaa gacctgcgcc gtcaaggccc
      121 cgggcttcgg cgaccgtcgc aaggccatgc tggaagacat cgccatcctg accggcggcc
      181 aggtcatcgc cgaggaagtc ggcctgaccc tcgagaaggc caccctggac gacctcggcc
      241 aggctgcccg catcgaagtc ggcaaggaaa acaccatcat catcgacggc gccggccagg
      301 ccgaccgcat cgaggcccgc gtcaagcaga tccgcgtgca gatcgaggaa gccagctccg
      361 actacgaccg cgagaagctg caggaacgcg tggccaagct ggccggcggt gttgcggtga
      421 tcaaggtcgg tgccgccacc gaagtcgaga tgaaggagaa gaaggcccgc gtcgaggacg
      481 ccctgcacgc cacccgcgct gcggtggaag agggcatcgt ccccggcggc ggcgtcgcgc
      541 tgctgcgtgc ccgcgccgcg ctgggcgagc tcaagggtga caaccacgac caggatgccg
      601 gcatcaagat cgtgctgcgc gccatggaac agcccctgcg cgagatcgtc gccaacgccg
      661 gcgacgagcc gagcgtggtg gtgaacaagg tcgtcgaggg ctcgggcaac tacggcttca
      721 acgccgccac cggcgagtac ggcgacatgg tcgagatggg cgtgctggat ccgaccaagg
      781 tcacccgcac cgctctgcag aacgccgcgt ccgtcgccgg cctgatgctg accaccgact
      841 gcatggtcgg cgagctggcc gaggacaagc cgatgggcgg catgcccgac atgggcggca
      901 tgggcggcat gggcggcatg ggcggcatgg gcatgtaact gcctgctcgg gaaggtccgg
      961 cccgccggac ctcccgcctg ctgcaagcga acaaggcctc gccggggaat gccctcggcg
     1021 gggccttttt gcgtccgtcg gttccggcac ggcgcgggcc gtcccgcgtc cagccgccgg
     1081 ggggggggct gcctgcgact gcgcaaagcg tgggataatt atccgattcg gggagtaaaa
     1141 gtcggtgggg agcgccgcgg cccagacggg cgggcgctcc ccggaacggc gctgcgcgca
     1201 gcagccacgt ccccgaagac cgagcaaaag gagaacagcg ggaacattct ctgaatctcc
     1261 gcttacaagc aacttacagc cggaatgcta tgcgtatcct gttggcggaa gacgatccgg
     1321 tgatcgccga cggcatctgt cgcgccctgc ggcgcggtgg ttgtgcagtc gatcacgtgg
     1381 cggacggcat ggaggcggat gccgcgctcg cgggccaggg ttacgacctg ctcatcctcg
     1441 acctcggcct gccgcgcatg aacggcatcg aggtgctcaa gcgcttgcgc gcgcgcaagt
     1501 cggctcagcc ggtgctgatc ctgaccgcgc aggatggcgt cgacgaccgc gtgcgcgggc
     1561 tcgatgccgg tgccgacgac tacctcacca agcctttcgc cctgcccgaa ctggaagcgc
     1621 gcgtgcgtgc gcttacccgc cgcggcaccg gccagccgcg ctgcatcgag atcggccagc
     1681 tgagctacga ccaggccgac cgcgtggtca agatcggcgg ccagctcgtc gagctgtcgg
     1741 cgcgcgagat cggcctgctc gagatcttcc tgctgcgtgt cggccgcctg gtcagcaagg
     1801 accagctcgt cgaccacctg tgcggctggg gcgaggaggt gtcgagcaat gcgatcgagg
     1861 tctacgtcca tcgcctgcgc aagaagctcg aggacagcgg cgtgcgcatc gtgaccgtgc
     1921 gcggcctggg gtactgcctg gaaaaccccg atgctgcccc tgcggcgaaa gtccaggcct
     1981 gagcgcgcgc cgcgtggcgc aaacgcgccg cgcaagtccg ggccgccgaa ctcgctgttc
     2041 ggcgagatcc tcgactggat gctcgcgccg ctgctgttcc tgtggccgat ctcgatcatc
     2101 gtcacccaca acgtcgccga cagcatcgcc aatcagccct acgatctcgc actcgccgac
     2161 ggcgtgcgcg cgctcgcgcg cctggtcgag gtcgaggacg gccaggtggt ggtgcgcttt
     2221 ccggcgccgc cgcgcgcgct gttccgcgcc gaccaggacg acgtcgtcta ctaccaggtg
     2281 gccgacgtca atggcgtcac ggtcagcggc gacgccgaga tcccgcccat gccgacctcg
     2341 tccgcagtcg acggcgagga ggtgcagctg cgcgacgcca tgatccaggg cgaggaggtg
     2401 cgcatcgcct ggcgcttcgt gcgccccgac cctgcgcagg ccgatcgttt cgtgctcgtg
     2461 caggtcgccg agacgcgcaa caagcgcacc gacctcgcct cgcgcgtggt caccggggtg
     2521 ctgctgccgc agttcgccat catcccgctc gcggtggtgc tggtgtgggt gggcctgagc
     2581 cgcggcatcg cgccgctcaa ccggctgcag agcctgatcc gccgccgccg tcccaccgac
     2641 ctgtcgccgg tcgagccctc cagcgtgccc gaggaggtgc gcccgctcat catcgcctac
     2701 aacgacatga tggcgcgcct ggaggagaac ctgcaggcgc agcagcgctt catcgccgac
     2761 gccgcgcacc agatgcgcac gccgctgacc gggctcaaga tgcagaccga cctcgccatg
     2821 cacgagaccg accccgacca gctgcgccag tcgctcaccc gcatcgccga gagcaccgac
     2881 cgcgcagccc acctgatcaa ccagctgctc tcgctcgccc gcgccgaggc gagcttcgag
     2941 aagctctacg cggtggagac ggtggacctc gatgcgatcg tgcgcgaggt ggcgctggag
     3001 ctctatccgc gcgcgcaggc caagcggatc gacctcggtg tcgaggcggg cgacacggcc
     3061 ctgcgcgtgg agggcaaccc ggtgctgctg cgcgagatgc tgaagaacct ggtcgacaac
     3121 gcgatcaagt acaccccgcg cggtggcagc gtcaccgcgc gcacgcgcta cgccggctcg
     3181 ccggtgttcg aggtcgagga caacggtccc ggcatccccg aggccgaccg cgagcgcgtc
     3241 ttcgagcgct tctaccgcgt gctcggcagc ggcgtcgatg gctccggcct cggcctgccg
     3301 atcgtgcgcg agattgccga gctgcaccgc ggcaccgtca ccctggacgc cggcccgggc
     3361 ggacgcggca cgctggcgcg cgtggtcttc ccgcgcagcc acctgcagcc accgcggcag
     3421 gtgcagggcg atcacgacgc cctgggctga gctcttcggg cattttcgcc aaaccctttg
     3481 acaaaggaaa aagactggct ataatgcgga cctcgttggc cagttagctc agttggtaga
     3541 gcagcggatt gaaaatccgc gtgtccgtgg ttcgattccg cgactggcca ccagaatccc
     3601 gatgggccga ccgagtgtcg gcccatttgc tttgcggcgt caatttgcgg ccagaagtcg
     3661 ctgttccggc tcaattttgg ggcgcaggtg cggtgttata atccggctcg ctccgtccca
     3721 cccccctccc gcatgcaact cttcgccctc ggcttgaacc accacaccgc gccgctcgtc
     3781 atccgcgaac gcgtcgcgtt ccagcccgag cgcatggggc aggcgctgca cgatctggcc
     3841 catacggatt cggtgcgcga ggcggcgatc ctgtcgacct gcaaccgcac cgagctctat
     3901 ttcgccgccg agcagcccca gcacgcggcc gactggctcg ctcgtttcca ccgcctcgcg
     3961 ctcgccgagg tctcgcccta cctgtattcc tacccgcagc gcgacgcgat ccgccatgtc
     4021 ttccgggtgg cgagcgggct cgactcgatg gtgatcggcg agccgcagat cctcggccag
     4081 gtcaaggacg ccgcgcgcta cgccgaagag gcgggcaccc tgggcgtgct gttgcacaag
     4141 ctgttccaaa acaccttcgc ggtggccaag gaggtgcgct cgaccaccgc gatcggcgcc
     4201 aacacggtgt cgatggccgc tgcggccgtg catctgaccg agcgcatctt cgagcgcgtt
     4261 gccgaccagc acgtgctctt catcggcgct ggcgagatga tcgagctctg tgcggcccac
     4321 ttcggcggcg ctcggcccaa atccatggcg gtggctaacc gtaccgagga gcgtgccagg
     4381 gccctggcgg accgcttcgg cgccaccacc atgcgcatcg atcagattgg cgaggcgctg
     4441 ccgcgtttcg acgtcgtcgt gtcctgtacg gccgcgccgc tgcccatcgt cggcttgggc
     4501 atggtcgagc gcgcggtcaa ggcgcgccgc catcgcccga tggtgatggt cgatctggcc
     4561 gtgccgcgcg acatcgagcc cgagatcgcc gaactcgacg acatcttcct gtacaccgtg
     4621 gatgatctcg cccaggtggt ggaggccggc atggagtcgc gccagcaggc ggtgatcgag
     4681 gccgagcaga tcatcgccag ccgggtcgat ggcttcctgc actggatgtc ggcgcgcgag
     4741 acggtgccgg tgatccgttc gctgcgcgag cacgccgaga gcctgcgcca ggccgagctc
     4801 gagcgcgccg tgcgcctgct cgccaagggc gaggacccgc agcgcgtgct cgaggcgctg
     4861 tcgcacggcc tcaccaacaa gctgatgcac ggccccaccc gcttcctgaa ccaggccgag
     4921 ggcgagcaca aggtcgaagc cggccgcgtg gtgcgcgagc tcttcaacct gtcccgcgat
     4981 tcccactagc cgcacggctc cgcaggccgc cgcccgccgg cggcgtgtgc gcgtcctttc
     5041 tccgtcatga agcagagcat ccgcgacaaa ctcgaacacc tcgccaaccg gctcgaagag
     5101 ctcgaccgca cgctcgcctc cgaggagggc gcgcgcgaca tgaacgcctt ccgcgagctc
     5161 tcgcgcgagc gcgccgagat cgagccggtg gtggcgctgt accgcgagca tcgccaggcc
     5221 gaagccgact gcgcgaccgc gcgcgagctg ctcgccgacc ccgagatgcg tgagctcggc
     5281 gagctcgagc ttgccgaggg cgaggcgcgc atcgaggcgc tggaggccga gctgcagcgc
     5341 gcactgctgc cgcgcgatcc caacgacgcg cgcaacctgt tcctcgagat ccgcgccggc
     5401 accggcggcg acgaggcggc gctgttcgcc ggcgacctcc tgcgcatgta cacccgctac
     5461 gccgaacgcc agcgctggaa ggtggagatc gtgtcggcga gcgaatccga cctcggcggc
     5521 tacaaggagg tcatcgcccg cgtggtcggc aacggcgcct attcgaagct caagttcgaa
     5581 tccggcggcc accgtgtgca gcgtgtcccg gtgaccgaga cccaagggcg catccacacc
     5641 tccgcctgca cggtcgctgt gctgcccgag gccgacgagg tcgaggcggt ggacatcaat
     5701 cccgccgacc tgcgcatcga taccttccgc gcctcgggcg cgggcgggca gcacatcaac
     5761 aagacggact ccgcggtgcg catcacgcac attcccaccg gcatcgtcgt cgagtgccag
     5821 gacgatcgct cgcagcaccg caacaaggcg caggccatgg cggtgctcgc cgcgcgcctg
     5881 tacgacgcca gggtgcgcgc gcaacagagc gccgaggccg cgcagcgcaa gagcctggtc
     5941 ggcagcggcg accgctccga gcgcatccgc acctacaact tcccgcaggg gcgggtgacc
     6001 gaccaccgca
//
LOCUS       DAOQNN010000180         6002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7587_length_6002_cov_8.906844, whole genome shotgun sequence.
ACCESSION   DAOQNN010000180 DAOQNN010000000
VERSION     DAOQNN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7587_length_6002_cov_8.906844"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..762
                     /gene="leuE"
                     /locus_tag="PKV97_10300"
     CDS             94..762
                     /gene="leuE"
                     /locus_tag="PKV97_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HNB06308.1"
                     /translation="MYGITDLFTFILGTIFIVLLPGPNSLYVMAAASRWGVRAGYQGA
                     AGIFVGDTILMLLAVTGMASVLRATPELFMVIKYAGAAYLAWVGLALLRGALATWRRG
                     DETADAVGDGKPDYPPLRSTPHPFRTALLISLMNPKAILFFVSFFIQFVDPAYEHPAL
                     SFVILGVIVQVCSVLYLSTLIFAGARLAAQFRRHRRMAAGATAGVGGVFIGFGAKLAT
                     ATLG"
     gene            complement(759..3290)
                     /locus_tag="PKV97_10305"
     CDS             complement(759..3290)
                     /locus_tag="PKV97_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HNB06309.1"
                     /translation="MAYVHPTHARAAQRSLGVLGVGLAGVLALWWLPTRWAAGLAHYL
                     PLHLALETASIVAAVLVFAVAWLTPERSAAANTRILGCCFLGVAVLDFTHMLSYLGMP
                     DFITPSSPEKAIDFWVAARLLAALGMLTAVLLPWRVRPGLSPGLLLLAVGAYVGLAHA
                     LFLPRPEWAPRTFIPGQGLTGLKIGIEIGLVLLFAASAALLLRRMRAPCGFDAPALFG
                     AAGLMALSELFFTMYAQVTDLYNLAGHLYKIAAHALLFRALVVDGIRRPYAALERSRH
                     ELADNSARLADERQRLESILAGTRAGTWEWNVQTGATIFNARWAEMLGYRLDELEPLS
                     IDTWRRFAHPDDLADSDAAVRRHLRGETPYYEHETRMRHRAGHWIHVQDRGVLRTHTA
                     DGAPEWMFGTHIDITDRYRAEQERAGALKRLRQLSESVPGVLFECRVEDDGKVSFPYV
                     GRQIEALIGCRAEEVEHDAAAFYARLHPDDGARVRDEIRHARHGMHDWNASFRVVHPL
                     YGERRMRGEARCQPRTDGVGEWFGYLRDETGTHRDQERLRLGARVFDASEEGILVTDA
                     AVRIVDMNPAMEALSGHAKASLLGRNPRELGSGRHDAAFFAAMYASLARDGRWQGEIW
                     NRHASGEIYVVKASMSTVLDESGRIEHYVGVFTDISTLMAQRDHFSSLANFDALTGLP
                     NRRLLDDRLQQAVGNARRGKPLVVCMLDLDGFKPVNDSVGHAEGDRLLVELAQRMQHI
                     LRAGETLARLGGDEFVLLLVDGDFRPALERVLEVTRAPVALSGGRFARVTASIGASAY
                     RHGVEDGAQLLREADQALYRAKRAGRNRWVMFAPEAAAGVETPPA"
     gene            complement(3292..>6002)
                     /locus_tag="PKV97_10310"
     CDS             complement(3292..>6002)
                     /locus_tag="PKV97_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB06310.1"
                     /translation="QHLARGFPEAVAILVTDAQGKILASTLDTPPQWDIGDREYFRHA
                     REHRQDALAFSEPVIYKAGPRKVVVAYEAIRSPAGDFVGVVAVALDLQRLQDLLLGVD
                     AGRQGMVSIRRSDDGRLVLRLPDHGASRVADITSTEPFLAIRRGETSGSARYVGIADE
                     VERSFAFQALRDYPFYVVIGRAISEQFAPWRQTATVATIIALATLGLLLALQAALHRS
                     RRQLARSKRSFDALIDSRREATCAWRPDTTLLTCNERYAELLGTEAASLPGTRWIDNV
                     PEAKRAEVHATITRMLQGAGTLTTDRRLRQADGRTRWLRWLDLPVLDEEGRCVEVHSI
                     GQDITAQKEVELRLRQLALAVEQSPNTIVITDTDGRIEYVNEAFVRTTGYTAAEAIGQ
                     NPRLLNAGKTPAATYADLWATLGRGEVWRGEFINTRKDGGTYTELATIAPIKQADGSV
                     SHYVAIKEDITGRREAEARIRQLAYYDTLTGLPNRSLMWDRLRHAIAASARSGASGML
                     MLLDIDHFKLLNDTQGHEVGDALLREVAERLRGALREEDTVARVGDDDFAIVIENLGA
                     DRDEAIGRAEKIAEHLHRCVTAPCELGLASGPYHVGASLGLTLFRGRNAAADAVLKQA
                     EVAVARAKDDGRNLIRFFSEAMQAVVAARAELELKLRAALAGNGFRLYYQPQLDRNGR
                     VIGAEALIRCFDAEGEMISPAAFIPLAEETGLIVPIGEWVLETACAQLRAWQRTPSTA
                     GLSLSINVSARQFHQPDFVGKVAAAIERHRIRPGGLEIELTESVVIGDIETTVLRMRQ
                     IKALGVRFALDDFGTGYSSLSYLKRLPFDQLKIDQMFVRDMEKDTSSEAIVRAILALS
                     RSLDLKVVAEGVETAAQHELLLTRGCELFQGYLFGRPVPIEDWKAG"
BASE COUNT          815 a         2083 c         2202 g          902 t
ORIGIN      
        1 cggtgcgaat gcgctgcttg ctggccgtga ccggccgggc gtggcaaagt aagggccggc
       61 tccgactccc aacccgaccc cgcgcgcgcc aacatgtacg gcatcaccga cctcttcacc
      121 ttcatcctcg gcaccatctt catcgtcctg ctgcccggcc ccaactcgct ctacgtgatg
      181 gcggcggcct cgcgctgggg ggtgcgcgcc ggctaccagg gcgcggcggg gatcttcgtc
      241 ggcgacacca tcctgatgct gctcgcggtg accggcatgg cctcggtgct gcgggccacg
      301 cccgagctgt tcatggtgat caagtacgcg ggtgcggcct acctggcctg ggtggggctg
      361 gccctgctgc gcggcgcgct ggcgacctgg cggcgcgggg acgagacggc ggacgcggtc
      421 ggcgacggca agcccgacta tccgccgctg cgcagcacgc cgcacccctt ccgcaccgcg
      481 ctgctgatca gcctgatgaa cccgaaggcg atcctgttct tcgtgtcctt cttcatccag
      541 ttcgtcgatc cggcctacga acacccggcg ctgtccttcg tgatcctggg tgtgatcgtg
      601 caggtgtgca gcgtgctcta cctgtccacg ctgatcttcg ccggcgcgcg cctggctgcg
      661 cagttccgcc gccaccgccg catggctgcg ggggcgaccg cgggggtggg cggggtcttc
      721 atcggcttcg gcgccaagct cgcgaccgcg acgctgggct aggccggcgg ggtctccacc
      781 ccggctgcag cttccggcgc gaacatcacc cagcggttgc gcccggcgcg cttggcgcgg
      841 tagagcgcct ggtcggcctc gcgcagcagc tgtgcgccat cctcgacgcc gtgccggtac
      901 gcgctcgcgc cgatgctcgc ggtgacgcgg gcgaagcgcc cgccggacag cgccaccggc
      961 gcgcgcgtca cctcgagcac gcgctcgagt gccgggcgga agtcgccgtc caccagcagc
     1021 aggacgaact cgtcgccgcc gaggcgggcc aaggtctcgc ccgcgcgcag gatgtgctgc
     1081 atgcgctgcg cgagctcgac cagcaggcgg tcgccctcgg cgtggccgac gctgtcgttg
     1141 accggcttga agccgtcgag gtcgagcatg cacacgacca gcggcttgcc gcggcgcgca
     1201 ttgcccaccg cttgctgcag gcggtcgtcg agcaggcggc ggttgggcag gccggtgagg
     1261 gcgtcgaagt tggcgagcga gctgaagtgg tcgcgctgcg ccatcagcgt gctgatgtcg
     1321 gtgaacacgc cgacgtaatg ctcgatgcgg ccggactcgt cgagcacggt ggacatcgac
     1381 gccttcacca cgtagatctc gcccgatgca tggcggttcc agatctcgcc ctgccagcgc
     1441 ccgtcgcgcg ccaggctggc gtacatcgcg gcgaagaagg ccgcgtcgtg tcggcccgag
     1501 ccgagctcgc gcgggttgcg cccgagcagg gacgccttgg catgcccgct gagcgcctcc
     1561 atcgccggat tcatatccac gatgcgcacc gcggcgtcgg tgaccaggat gccctcctcg
     1621 ctggcgtcga agacgcgtgc gcccaggcgc aggcgctcct ggtcgcggtg cgtcccggtc
     1681 tcgtcccgga gatagccgaa ccactcgccg acgccgtcgg tccgcggctg gcagcgcgcc
     1741 tcgccgcgca tccggcgttc gccgtacagc gggtgcacca cgcggaagga ggcgttccag
     1801 tcgtgcatgc cgtggcgcgc atggcggatc tcgtcccgca cccgtgcgcc gtcgtcggga
     1861 tgcaggcggg cgtagaaggc tgcggcgtca tgctccacct cttctgcgcg gcagccgatc
     1921 agcgcctcga tctggcggcc gacgtagggg aagctcacct tgccgtcgtc ttccacgcga
     1981 cactcgaaga gcacgccggg cacgctctcc gagagctgac gcaggcgctt cagcgccccg
     2041 gcgcgctcct gctcggcgcg gtagcggtcg gtgatgtcga tatgcgtgcc gaacatccat
     2101 tccggcgcgc cgtcggcggt gtgggtgcgc agcacgccgc gatcctgcac gtggatccag
     2161 tgcccggcgc ggtggcgcat gcgggtctcg tgctcgtaat acggcgtctc gccgcgcagg
     2221 tgacggcgca ccgcggcatc cgagtcggcg aggtcgtccg ggtgggcgaa gcgccgccag
     2281 gtgtcgatcg acagcggttc gagctcgtcg aggcgatagc cgagcatctc cgcccagcgc
     2341 gcgttgaaga tcgtcgcccc ggtctgcacg ttccattccc aggtgccggc gcgcgtgccc
     2401 gcgaggatgg actccaggcg ctggcgttcg tcggcgaggc gcgcggagtt gtccgcgagt
     2461 tcgtgccgcg agcgttcgag cgcggcatag gggcggcgga tgccgtccac caccagcgcg
     2521 cggaagagga gggcgtgcgc ggcgatcttg tagagatggc cggcgaggtt gtacaggtcg
     2581 gtgacctgcg cgtacatcgt gaagaagagc tcgctcagcg ccatcagccc ggccgcaccg
     2641 aacagggcgg gggcgtcgaa gccgcagggc gcgcgcatgc ggcgcagcag caaggcggcg
     2701 gatgcggcga acagcagcac caggccgatc tcgatgccga tcttcagccc cgtcagcccc
     2761 tggcccggga tgaaggtgcg cggcgcccac tcgggccgcg gcaggaagag cgcgtgcgcc
     2821 aggcccacgt aggcgcccac cgcgagaagc agcaggccgg gagacaggcc ggggcgcacc
     2881 cgccacggca gcagcaccgc cgtcagcatg ccgagcgcag ccagcaggcg cgcggcgacc
     2941 cagaaatcga tcgccttctc ggggctgctc ggcgtgatga agtccggcat gccgaggtag
     3001 gacagcatgt gagtgaaatc cagcaccgcc acgccgagga agcagcaccc caggatgcgc
     3061 gtgttggccg ccgcgctgcg ctcgggcgtc agccatgcga ccgcgaacac cagcacggcg
     3121 gcgacgatcg aggcggtctc cagcgccagg tgcagcggca ggtagtgcgc cagcccggcg
     3181 gcccagcgcg tgggcagcca ccacagcgcg agcacgccgg cgaggccgac gccgagcacg
     3241 ccgaggctgc gctgcgcggc gcgggcgtgg gtcgggtgga cgtacgccat ctcagcctgc
     3301 cttccagtcc tcgatcggaa ccggccggcc gaacagatag ccctggaaca gctcgcagcc
     3361 gcgcgtcagc agcagctcgt gctgggcggc ggtctcgaca ccctcggcca ccaccttcag
     3421 gtcgagcgag cggctgagcg cgaggatcgc gcgcacgatc gcctcgctgc tggtgtcctt
     3481 ctccatgtcg cgcacgaaca tctggtcgat cttgagctgg tcgaagggca ggcgcttgag
     3541 ataggacagc gaggaatagc cggtgccgaa gtcgtcgagt gcgaacctca cccccagggc
     3601 cttgatctgg cgcatgcgca gcaccgtggt ctcgatgtcg ccgatcacca cgctctcggt
     3661 gagctcgatc tcgaggccgc cggggcggat gcggtggcgc tcgatcgccg ccgccacctt
     3721 gccgacgaag tcgggctggt ggaactggcg cgcgctgacg ttgatcgaca ggctcagccc
     3781 ggccgtggag ggcgtgcgct gccatgcacg cagttgcgca caggcggtct ccagcaccca
     3841 ctcgccgatc ggcacgatga ggccggtctc ctcggcgagc gggatgaagg cggcgggcga
     3901 gatcatctcg ccctcggcgt cgaagcagcg gatcagcgcc tcggcaccga tcacgcggcc
     3961 gttgcggtcg agctgcggct ggtagtacag gcggaagccg ttgccggcca gcgccgcgcg
     4021 cagcttcagc tccagctcgg cgcgcgcggc caccaccgcc tgcatcgcct cgctgaagaa
     4081 gcggatcagg ttgcggccgt cgtccttggc gcgcgccacc gccacctcgg cctgcttgag
     4141 caccgcgtcg gccgccgcgt tgcggccgcg gaacagggtc aggcccaggc tcgcgccgac
     4201 gtggtagggg ccgctcgcga ggccgagctc gcagggcgcg gtcacgcagc ggtgcaggtg
     4261 ctcggcgatc ttctcggcgc ggccgatcgc ttcgtcgcgg tccgcgccca ggttctcgat
     4321 cacgatcgcg aagtcgtcgt cgcccacccg cgccaccgtg tcctcctcgc gcagcgcgcc
     4381 gcgcaggcgc tccgccacct cgcgcagcag ggcatcgccc acctcgtggc cctgggtgtc
     4441 gttgagcagc ttgaagtggt cgatgtcgag cagcatcagc atgccgctcg cgccgctgcg
     4501 cgcgctcgcc gcgatggcgt ggcgcaggcg gtcccacatc aggctgcggt tgggcaggcc
     4561 agtgagggtg tcgtagtagg cgagctggcg gatgcgcgcc tcggcctcgc ggcgcccggt
     4621 gatgtcctcc ttgatcgcca catagtggct gacgctgccg tcggcctgct tgatcggcgc
     4681 gatcgtggcg agctcggtgt aggtgccgcc gtccttgcgg gtgttgatga actcgccgcg
     4741 ccacacctcg ccgcggccga gcgtggccca caggtcggca taggtggcgg cgggggtctt
     4801 gccggcgttg agcaggcgcg ggttctggcc gatcgcctct gcggcggtgt agccggtggt
     4861 gcgcacgaag gcctcgttga catactcgat gcgaccatcg gtgtcggtga tcacgatcgt
     4921 gttcgggctc tgctcgacgg ccagtgcgag ctggcgcagg cgcagctcga cctccttctg
     4981 cgcggtgatg tcctggccga tcgagtgcac ttcgacgcag cgcccctcct cgtcgagcac
     5041 cggcaggtcg agccagcgca gccagcgcgt gcggccgtcg gcctggcgga ggcggcggtc
     5101 ggtggtgagc gtgccggcgc cctgcagcat ccgcgtgatg gtggcgtgga cctccgcgcg
     5161 cttcgcctcc ggcacgttgt cgatccagcg cgtgcccggc agggacgcgg cctcggtgcc
     5221 gagcagttcg gcgtagcgct cgttgcaggt cagcagcgtg gtatcgggcc gccaggcgca
     5281 ggtcgcctcc cggcggctgt cgatgagggc gtcgaagctg cgcttgctgc gcgcgagctg
     5341 gcggcggctg cggtgcaggg cggcctgcag cgccagcagc aggcccaggg tggccaaggc
     5401 gatgatcgtg gcgacggtcg cggtctgccg ccagggcgcg aattgctcgg agatcgcgcg
     5461 gccgatcacc acgtagaagg ggtagtcgcg cagcgcctgg aaggcgaagc tgcgctcgac
     5521 ttcgtccgcg atgccgacgt agcgcgcgct gccggacgtc tcgccgcggc ggatggccag
     5581 gaaaggctcg gtgctggtga tgtcggcgac ccggctggcg ccgtggtccg gcaagcgcag
     5641 caccaggcgg ccgtcgtcgc tgcgccggat gctgaccatg ccctggcgac cggcgtccac
     5701 cccgagcagc aggtcctgca ggcgctgcag gtcgagcgcg accgcgacca cgccgacgaa
     5761 atcgcccgcc gggctgcgga tcgcttcgta ggccaccacg accttgcgcg ggccggcctt
     5821 gtagatcacg ggctcggaga aggcgagcgc gtcctggcgg tgttcgcggg cgtggcggaa
     5881 atattcgcgg tcgccgatgt cccattgcgg cggcgtgtcg agggtgctgg ccaggatctt
     5941 gccctgcgcg tcggtcacca ggatggcgac ggcttccgga aagccgcgcg ccagatgctg
     6001 ca
//
LOCUS       DAOQNN010000181         5952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7702_length_5952_cov_8.874512, whole genome shotgun sequence.
ACCESSION   DAOQNN010000181 DAOQNN010000000
VERSION     DAOQNN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7702_length_5952_cov_8.874512"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /gene="glnA"
                     /locus_tag="PKV97_10315"
     CDS             <1..580
                     /gene="glnA"
                     /locus_tag="PKV97_10315"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678638.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HNB06311.1"
                     /translation="DGKNLFAGNGYAGLSETALYYIGGIIKHARALNAITNPGTNSYK
                     RLVPHYEAPTKLAYSARNRSASIRIPYVANPKGRRIESRFPDPLANPYMCFAALMMAG
                     LDGIQNKIHPGDPADKNLYDLPPEEDALIPTVCTSLEQALEYLDKDREFLTRGGVFSN
                     DFIDAYIALKMEEVDRMRITTHPVEFDMYYSL"
     gene            717..1202
                     /locus_tag="PKV97_10320"
     CDS             717..1202
                     /locus_tag="PKV97_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNB06312.1"
                     /translation="MRPYRILPLLLLAGLAAPAHAEIYKCTDADGRVTYTNDRALARG
                     CVRLQSDQPVSSVPAPVRRPAAPAARETPTGGDFPRVSPNEQRGRDDARRQVLQEELR
                     TEEQKLAEAEKALAEQESIRLGDERNYQKVLDRVRPFKDRVELHKRNVDALRREISGL
                     R"
     gene            1256..2332
                     /gene="glnL"
                     /locus_tag="PKV97_10325"
     CDS             1256..2332
                     /gene="glnL"
                     /locus_tag="PKV97_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HNB06313.1"
                     /translation="MPSHPTHAASSPSSPFAGLDLLSSAVVLVDAGLVIRYLNAGAEN
                     LFAISRRKLLGQPLERLLGSPPGLAAALDNALRTNWSYTGQDISVQRGDAEPLRLDCT
                     VTPVDTASVRLLLEFRPIDAQLRVAREEQLLHQQQANRELIRNLAHEIKNPLGGIRGS
                     AQLLQHELDDPQLREFTDVIIAEADRLQDLMNRLLSSHCMMRPASINLHDVLERVRRL
                     ILAEFPSIGIVPDYDLSLPELTADREQLIQAVLNIVRNAAQALGGRGEILLRTRIARQ
                     VTLAKRRHKLALKLQVIDDGPGIPEEIRDRIFYPLVSGREGGSGLGLSLAQSFIEQHQ
                     GMIEVDSRPGRTCFTILLPITERA"
     gene            2359..3783
                     /gene="ntrC"
                     /locus_tag="PKV97_10330"
     CDS             2359..3783
                     /gene="ntrC"
                     /locus_tag="PKV97_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HNB06314.1"
                     /translation="MNTVWIIDDDRSIRWVLEKALGREGIDHASFSSAGDALAELERA
                     PQPPAALLSDIRMPGESGLDLLQKVKERHPQLPVIIMTAYSDLDSAVAAFQGGAFEYL
                     PKPFDVDQAVALVRRALEQTAHQNGASEEATLAPEILGQAPAMQEVFRAIGRLAHSHA
                     TVLINGESGSGKELVARALHRHSPRRDAPFIAINTAAIPRDLLESELFGHERGAFTGA
                     ATQRRGRFEQADGGTLFLDEIGDMPAELQTRLLRVLSDGHFYRVGGQQPIRANVRVIA
                     ATHQDLEERVRQGLFREDLFHRLNVIRLRLPPLRERHEDIPLLVRHFLQKSAQELGVE
                     RKRISEATLEYLQAQPFPGNVRQLENLCHWLTVMAPAQVVEVADLPPEMREQPGRESP
                     SNWMEGLGSEADRLIASRPGEVFDRLTRDFERTLIRRALAATGGRRIEAAQLLGIGRN
                     TITRKIQELGMDEERAPEAEESGR"
     gene            3967..4749
                     /locus_tag="PKV97_10335"
     CDS             3967..4749
                     /locus_tag="PKV97_10335"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNB06315.1"
                     /translation="MASLLGARARDFALEWVECCDSTNARLVDAPPADDGRVHVLVAD
                     RQTAGRGRRGRQWLSWEGASLTFSLLWRFAPGTPAPAGLSLAAGLALARALEQLGVEG
                     VQLKWPNDVLVHGEKLAGILVELLPVRGRPPAAVIGIGLNLRLPADAAIPDQAGVTDL
                     AAALGGEPPSRPVLLAAILGEFHRLFDTYAAAGFPALRGAWEQRNAFAELPVAVRGES
                     ATLQGTCAGVDDDGALLLRTGDGVQRVLAGDVSLRPLLDGSR"
     gene            4746..5483
                     /locus_tag="PKV97_10340"
     CDS             4746..5483
                     /locus_tag="PKV97_10340"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNB06316.1"
                     /translation="MILLVDAGNTRVKWRVIAADAPARALAEGAVGHAEIDMLAAACR
                     AWPGLARVFGCNVAGTSVAARIATACAPLRVDWLLPTAARAGVRNLYENPERLGADRW
                     AALIGARHSHAGPCLVVTAGTATTVDLLSAAGDFLGGLILPGVELMQQALARGTAQLP
                     LAEGHFAWQPRRTVDAIRSGCLQAQTGAVERMFRQIAEHPGALCLLGGGAADSFADLL
                     EIPLRRTDNLVLTGLGTVATLPAPPTH"
     gene            5510..>5952
                     /locus_tag="PKV97_10345"
     CDS             5510..>5952
                     /locus_tag="PKV97_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNB06317.1"
                     /translation="MEATRILLDAADIPTHWYNVAADLPTPLAPPLGPDGHPATPEQM
                     GVIFPPAILEQEMSTERWIAIPQEVREIYRLWRPSPLCRAVRLEQALGTPAKIFFKYE
                     GVSPAGSHKPNSAVPQAFYNKQAGITRLTTETGAGQWGSSIAFAG"
BASE COUNT          921 a         2292 c         1920 g          819 t
ORIGIN      
        1 ggacggcaag aacctgttcg cgggcaacgg ctacgccggc ctgtccgaga ccgcgctgta
       61 ctacatcggc ggcatcatca agcacgcccg cgcgctcaac gcgatcacca acccgggcac
      121 caactcgtac aagcgcctgg tgccgcacta cgaggccccg accaagctcg cctactcggc
      181 gcgcaaccgc tcggcctcga tccgcatccc ctacgtcgcc aacccgaagg gccgccgcat
      241 cgagtcgcgc ttcccggatc ccctggccaa cccgtacatg tgcttcgccg cgctgatgat
      301 ggcgggcctg gacggcatcc agaacaagat ccacccgggc gacccggccg acaagaacct
      361 gtacgacctg ccgccggaag aggacgcgct gatcccgacc gtgtgcacca gcctcgagca
      421 ggcgctggag tacctggaca aggatcgcga gttcctgacc cgcggcggcg tgttctcgaa
      481 cgacttcatc gacgcctaca tcgcgctcaa gatggaagag gtcgatcgca tgcgcatcac
      541 cacccacccg gtggaattcg acatgtacta cagcctgtaa gccgggaaat cctgcgcagg
      601 acggaaggga cgggcgttgc ccgtcccttt tttctggggt ctaatcgggc ctgacccgca
      661 aggatgacgg cattgcagcc cgttactgcc gtccccgcgc ccctccggac ctgcgcatgc
      721 gaccgtatcg aatcctcccg ctgctcctgc tcgccggcct tgccgcgccg gcccatgccg
      781 aaatctacaa gtgcaccgat gccgacggca gggtgaccta caccaacgat cgcgcactcg
      841 cgcgcggttg cgtgcgcctg cagtccgacc agccggtgtc ctcggtgccc gcccccgtgc
      901 gccgtcccgc cgctccggcg gcacgcgaga cgcccacggg cggcgacttc ccgcgcgtga
      961 gccccaacga gcagcgcggc cgtgacgacg cccgccgcca ggtgctgcag gaagaactgc
     1021 gcaccgagga gcagaagctg gccgaggccg agaaggcgct cgccgagcag gaatccatcc
     1081 gtctcggcga cgagcgcaac taccagaagg tgctcgaccg cgtgcgaccc ttcaaggacc
     1141 gcgtcgaatt gcacaagcgc aatgtcgatg cactgcgccg ggagatctcc ggcctgcgat
     1201 gacccggacc tggcgcgctt cttgcatgtc ctcgaccgaa caacaggacg cgcccatgcc
     1261 atcgcacccc acccacgccg ccagcagccc cagcagcccg ttcgccggac tcgacctgct
     1321 gtcttcggcg gtcgtgctcg tcgacgccgg gctggtgatc cgctacctca acgcgggcgc
     1381 ggagaacctg ttcgcgatca gccggcgcaa gctgctcggc caaccgctcg agcgcctgct
     1441 cggcagcccg cccggcctgg ccgcggcgct cgacaacgcg ctgcgcacca actggagcta
     1501 caccggccag gacatcagcg tgcagcgcgg cgacgccgag ccgctgcggc tcgactgcac
     1561 ggtgacgccg gtcgacaccg caagcgtgcg cctgctgctc gaattccgcc cgatcgacgc
     1621 gcagctgcgc gtcgcgcgcg aggagcagct gctgcaccag cagcaggcca accgcgagct
     1681 catccgcaac ctcgcgcacg agatcaagaa cccgctcggc ggcatccgcg gctcggccca
     1741 gctgctgcag cacgagctcg acgacccgca gctgcgcgaa ttcaccgacg tcatcatcgc
     1801 cgaggccgac cgcctgcagg acctgatgaa ccgcctgctg agttcgcatt gcatgatgcg
     1861 cccggcctcg atcaacctcc acgacgtgct cgaacgcgtg cgccgcctga tcctcgccga
     1921 gtttccttcg atcggcatcg tccccgacta cgacctcagc ctgcccgagc tcaccgccga
     1981 tcgcgagcag ctcatccagg ccgtgctcaa catcgtgcgc aacgccgcac aggcgctggg
     2041 cggccgcggc gagatcctgc tgcgcacccg catcgcgcgc caggtcacgc tcgccaagcg
     2101 ccgtcacaaa ctggcactca aattgcaagt aatcgacgac ggccccggca tccccgagga
     2161 gatccgcgat cgcatcttct atccgctggt ttcggggcgg gagggcggca gtggcctggg
     2221 cctgtcgctc gcacagagct tcatcgagca acaccagggc atgatcgagg tggatagccg
     2281 tcccgggcgc acctgcttca cgatcctgct gccgattacc gagcgtgcct gagcgcgcac
     2341 cagaatagtg catacgatat gaacaccgtc tggatcatcg acgacgaccg ctcgatccgc
     2401 tgggtgctgg aaaaggcgct cggccgcgag ggcatcgacc acgccagctt cagctccgcc
     2461 ggcgacgcgc tcgccgagct cgagcgcgca ccgcagccgc cggccgcgct gctatccgac
     2521 atccgcatgc ccggcgagtc cgggctggac ctgctgcaga aggtgaagga gcgccacccg
     2581 cagctgccgg tcatcatcat gaccgcctac tccgacctcg acagcgcagt cgcggccttc
     2641 cagggcggcg ccttcgagta tctgcccaag cccttcgacg tcgaccaggc ggtcgcgctc
     2701 gtccgtcgtg cgctggagca gaccgcgcac cagaacggtg cgagcgaaga ggccaccctg
     2761 gcccccgaga tcctcggcca ggccccggcg atgcaggagg ttttccgcgc catcgggcgg
     2821 ctcgcccact cgcacgccac cgtgctgatc aacggcgagt cgggcagcgg caaggaactg
     2881 gtcgcgcgcg ccctgcaccg ccacagcccg cgccgcgacg cgcccttcat cgccatcaac
     2941 accgcggcca tcccgcgcga cctgctcgaa tccgaactct tcggccacga gcgcggcgcc
     3001 ttcaccggcg ccgccaccca gcgccgcggc cgcttcgagc aggccgacgg cggcacgctg
     3061 ttcctcgacg agatcggcga catgccggcc gagctgcaga cgcggctgct gcgcgtgctc
     3121 tccgacggcc acttctaccg cgtcggcggc cagcaaccga tccgcgccaa cgtgcgcgtg
     3181 atcgccgcca cccaccagga cctggaggag cgcgtgcgcc agggcctgtt ccgcgaggac
     3241 ctcttccacc gcctaaacgt catccgcctg cgcctgccgc cgctgcgcga gcgccatgag
     3301 gacatcccct tgctggtgcg gcacttcctg cagaagagcg cgcaggagct cggcgtcgag
     3361 cgcaagcgca tctcggaggc cacgctcgaa tacctgcagg ctcagccttt cccgggcaac
     3421 gtgcgccagc tcgagaacct gtgccactgg ctcaccgtga tggcaccagc gcaggtggtg
     3481 gaggtcgccg acctgccacc cgagatgcgc gagcaacccg gccgcgagtc gccatcgaac
     3541 tggatggagg gcctgggcag cgaggccgac cgcctgatcg cctcgcgccc cggagaggtg
     3601 ttcgaccgtc tcacgcgcga tttcgagcgc accctgatcc gccgtgcgct ggccgccacc
     3661 ggcggccgcc gcatcgaggc cgcgcagctg ctcggcatcg gccgcaacac catcacccgc
     3721 aagatccagg agctgggcat ggacgaggag cgcgcgccgg aggcggagga aagcgggcgc
     3781 tgatcgcccc ggtcgcctcg atgcgggggc gcgttggcgc ggccggcgca cggggtgcag
     3841 cgcatccatg ccgcgggaag cgcatccccc acggtcttgg cggataatgc ggttttcgat
     3901 ccgccgcttc cgcacttgtc ctccatccgt ccatccacgc cttcggcgcg cgcctgcgcg
     3961 accgaactgg cctccctcct cggcgcgcgt gcgcgcgact tcgccctcga atgggtggag
     4021 tgctgcgact cgaccaacgc ccgtctcgtc gatgcgccgc ccgccgacga cggtcgtgtg
     4081 catgtgctcg tggccgaccg ccagaccgcc gggcgcgggc ggcgcgggcg gcaatggctg
     4141 tcctgggagg gcgccagcct caccttctcg ctgctgtggc gcttcgcgcc gggcaccccg
     4201 gcgccggccg gactctcgct cgccgccggg ctggcgctcg cgcgcgcgct cgagcagctc
     4261 ggcgtcgagg gggtgcagct gaaatggccc aacgacgtgc tggtgcacgg agagaagctc
     4321 gccggcatcc tggtcgaact cctccccgtg cgcggtcgcc cgcccgcggc ggtgatcggc
     4381 atcggtctca acctgcgcct gccggccgac gccgccattc ccgaccaggc cggcgtcacc
     4441 gacctcgccg ctgcgctcgg cggcgaaccg ccctcgcgtc cggtcctgct ggcggccatc
     4501 ctcggcgagt tccaccgcct cttcgacacc tacgcggccg cgggcttccc cgccctgcgc
     4561 ggcgcctggg agcagcgcaa cgccttcgcc gagttaccgg tggcggtccg cggggagtcc
     4621 gccacgctgc agggtacctg cgccggggtg gacgacgacg gcgccctgct gctgcgcacc
     4681 ggggacggcg tgcagcgggt gctcgccggc gacgtgtcgc tgcgtccgct gctggacggc
     4741 tcgcgatgat cctgctcgtg gatgccggca acacccgggt gaaatggcgc gtgatcgcag
     4801 cggacgcccc cgcgcgagcc ctcgccgagg gggcagtcgg ccacgccgag atcgacatgc
     4861 tcgccgccgc ctgccgcgcc tggccgggac tcgcgcgcgt gttcggctgt aacgtcgccg
     4921 gcaccagcgt cgccgcgcgc atcgccacgg cctgcgcacc gctgcgggtg gattggctgc
     4981 tgcccaccgc cgcgcgcgcc ggcgtgcgca acctgtacga gaaccccgag cgtctcggcg
     5041 ccgaccgctg ggcggcgctg atcggtgccc gccacagcca tgccggcccc tgcctggtcg
     5101 tcaccgccgg caccgcgacc acggtggacc tgctctccgc ggcgggcgac ttcctcggcg
     5161 gcctgatcct gcccggggtc gagctgatgc agcaggcgct cgcccgcggc acggctcagc
     5221 tgccgctcgc cgagggtcac ttcgcgtggc aaccgcgacg cacggtagac gcgatccgct
     5281 ccggctgcct gcaagcccag accggcgcag tcgagcgcat gttccgccag atcgcggagc
     5341 accccggcgc gctctgcctg ctcggcggcg gcgccgccga cagcttcgcc gacctgctcg
     5401 agattccgct gcgacgaacc gacaatctgg tgctgaccgg cctgggcacg gtcgccaccc
     5461 tgcccgcccc gcccacccac tgacaagaat cgaaagggag aaaacaacga tggaagccac
     5521 ccgcatcctg ctcgacgccg ccgacatccc gacgcactgg tacaacgtcg ccgccgatct
     5581 gccgacgccg ctcgcgccgc cgctcggccc cgacggccat ccggccacgc cggagcagat
     5641 gggggtcatc ttcccgccgg cgatcctcga acaggagatg agcaccgagc gctggatcgc
     5701 catcccgcag gaggtgcgcg agatctaccg cctgtggcgg ccgagcccgc tgtgtcgcgc
     5761 ggtgcgcctg gagcaggcgc tcggcacccc ggcgaagatc ttcttcaagt acgagggcgt
     5821 ctcgcccgcc ggctcgcaca agcccaactc cgcggtgccg caggccttct acaacaagca
     5881 ggccggcatc acccgcctga ccaccgagac cggcgccggg cagtggggct cgtcgatcgc
     5941 cttcgccggg ca
//
LOCUS       DAOQNN010000182         5948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7717_length_5948_cov_6.129815, whole genome shotgun sequence.
ACCESSION   DAOQNN010000182 DAOQNN010000000
VERSION     DAOQNN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7717_length_5948_cov_6.129815"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKV97_10350"
     CDS             complement(<1..556)
                     /locus_tag="PKV97_10350"
                     /inference="COORDINATES: protein motif:HMM:NF039746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6384 family protein"
                     /protein_id="HNB06318.1"
                     /translation="MNDLASRDPQSVPELLRIMDVATALRRERERAERELAIDETKDK
                     LRERLRAAARESGDPVSDAEIETAIAQYFRAQFEFADPKPGWRRLVALSWIHRGRVAV
                     VTGVIVAAIVGLRMLVFAPGAPLSSAGRVQRATEAAHAPLSAAVDRLRSLAKGDEARA
                     AVDQIAARAASTYAKQDVSGMESVK"
     gene            919..>1436
                     /locus_tag="PKV97_10355"
     CDS             919..>1436
                     /locus_tag="PKV97_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06319.1"
                     /translation="MSNAFSAAELRSQALGVQPHMAPINAGALAAGYNQPGPTPQMNV
                     PDTIWNNGEGFRANSTADADYGAHLGQCDPGVELGDIFAVEQTIGGTPGWRQVTHIDQ
                     KTGATMHLWRFRGYLRPGQLLPYPTPPSTGPWTIKDAAFELFLPDPLPSTTQILLWIT
                     PWSTTGKPSPSDG"
     assembly_gap    1437..1536
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2034..5264
                     /locus_tag="PKV97_10360"
     CDS             2034..5264
                     /locus_tag="PKV97_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06320.1"
                     /translation="MLADQFNFEYLATRTGFSSYGGAYSPHDTLEWGTAFRFMDQTPG
                     SPGWDYFNLSPLIRKRRGELYAAMAWVCQDEDTIGHGTDWMSLLTGTRNYTPRFASTS
                     APLLYFTAVDRRCHESGWHTTAIGGVGGAWLGRSDAIEMAPVAYTNWSNQNPVVPPLI
                     QDDGSEDSYAWALDRALPADYVPNPGDKLLLDTSFGSPASPGRTEGAGLALGLDESLR
                     FADVPGDGGGKSIFTGSADGVVTRFVLNSATKAFDVAAQSVPLGFGAFALAVGDLSDG
                     HAGLEVAVGTKEHLFTLDALTLSVLNWREVPRTFEHARPRRMQIADVFSGPDFPGKEV
                     ITTTLMGHVLVFSGDGFGDLVDFGEPGVQDLAVLEGHVSAASTTTSNTPILLLSHRGH
                     LAHVTLNKVADPTQRNPGPAELQAWTAGEEGTPMDLEIVSAPVGGGPVAVALYAHQGT
                     DGTNDVPQIRCFDALTMHPTSMATHGIPPSLGWFELGTLGRDVLAADLAPVHLSSGAL
                     AGFVVMLSARIVWVPINGSQPQQPAGYKLDGFAPASRAIAVTTADIATRSGGETYKEE
                     IILSTLGGHVVWFHLEDMLANGPSVYLDFVGTPPPPPIPLGVIPFTNRNSAGQWGLLA
                     HDEGNGTHLFGANQSGGLYETDPTSGLSVMRDVFREAGNVVGTSSRVPSIVTAPVRDL
                     VQIGAGVQQGAGTIEARTYTTTSAGSGLRWLQTRPWQDAINDVLRPVWFQDHTEINGI
                     VYNTEARMPVINGFAALIGAGDAIATGSPIAGATRQLHWWGGDFDDFTNLIQGAYFDA
                     THVIDNWYSTRNVPGGVPPVPPQQNWTGRTSGDCKDLRNEIRQWAQINNMQSLRLGTD
                     AFGTVVVASTEGGSVVLLRPGRDAGQAGQDYGTILWDSADPQTPADEGMNGMGLAVRA
                     VPNDPSHSLDIFLGVGVTHLDPQHWLNGSGPGNLTGGIAWYRWTGSSATSGTMARNGL
                     LHLDSNSGNPIGARGGFGVCGLAIGDVISTQAGDELVATTLEGDLFVFTIPTSGMLNP
                     GNILHRAWARGALGVNNGIAIFDSNNDGKKELFIAGSLGIWKWRQP"
     gene            5261..>5948
                     /locus_tag="PKV97_10365"
     CDS             5261..>5948
                     /locus_tag="PKV97_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06321.1"
                     /translation="MSLQLETSIDSICVRVLECLLRWAHRLALFCGIAWIAAEALPAQ
                     RTWVVDDTMAAGTDFPDLVSAYAAAVPGDRIHIRFGSGQGYRAPASINKALTIVGLGT
                     TKPIVVHSLVPISCPAQQVLVFDNLDFRADANGLATSVENSLGTVLLSRISYPGALYD
                     PVNTWNAHRVVMVDCVVTAPVQAVAVQGSKVWLLNCVTRARSAGDPNYTGDIQIRYGG
                     FLTVVGGQHQG"
BASE COUNT          950 a         2038 c         1878 g          982 t
ORIGIN      
        1 ccttgaccga ctccataccg gacacgtcct gcttcgcgta tgtcgatgcc gcgcgtgcgg
       61 cgatctgatc gacggcggcg cgcgcttcgt cgcctttcgc cagcgaccgc agtcgatcga
      121 ccgcggcgct cagcggcgca tgggccgctt ccgtcgcacg ctgcacacgc cccgccgacg
      181 acaggggcgc acccggcgcg aagaccagca tccgcagtcc gacgatcgcc gcgacgatca
      241 cgccggtcac caccgccacg cgcccgcggt ggatccagct cagcgccacg agccgtcgcc
      301 accccggctt cgggtcggcg aactcgaact gcgcgcggaa gtactgcgcg atcgccgtct
      361 cgatctccgc atcgctgacc ggatcgccgc tctcccgcgc cgccgcccgc agccgctcgc
      421 gcagcttgtc cttggtctcg tcgatcgcca gctcccgctc cgcgcgctcg cgctcgcgcc
      481 gcagcgccgt cgccacgtcc atgatccgga gcaactccgg aacactctgc ggatcgcggc
      541 tggcgaggtc gttcatgggg acgatgtggc ggcggggatg atcgatgggg tccggggcga
      601 agacaagcgg agtgctacgc ccgacttggg gctcctgttc agcagcggtc gcaaccgcga
      661 accgcagccg aggcgcgctc ggatgggccc gtggccgtcg ggcgagcggt gcctccagtg
      721 tgaacagggt cggcaagcga gggggggatt gccaatggaa cagggtctgc acatcgtctc
      781 ctccctcgct cccgcaaagt gcggacgcga ttccaggagg accgacgtga agccacggga
      841 agcttcgttg cgggtgcatg cacgggaagg catgcgtctc tctctctctc tctctctctc
      901 tctctctccg cattgccgtt gagcaatgcg ttctccgccg ctgagttgcg gagtcaggcg
      961 ctcggcgtgc agccgcacat ggcgccgatc aacgccggag ccttggccgc gggctacaac
     1021 caacccgggc cgacgccgca gatgaacgtg cccgacacga tctggaacaa cggcgaaggc
     1081 ttccgcgcca actcgaccgc cgatgccgac tacggtgcgc acctcgggca atgcgatccg
     1141 ggagtcgaac tcggagacat cttcgcggtc gagcagacca tcggtggtac gcctggttgg
     1201 cggcaggtaa cccacatcga ccagaagacc ggcgccacga tgcatctgtg gcgcttccgc
     1261 ggctatctcc gcccggggca actcctgccc tatccgacac cgccgtcgac ggggccgtgg
     1321 accatcaagg atgccgcctt cgaactgttc ctccccgacc ccctgcccag cactacgcag
     1381 atcctcttgt ggatcacgcc ctggtcgacg accggcaagc cgagcccgag cgacggnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcct cttgtggatc acgccctggt
     1561 cgacgaccgg caagccgagc ccgagcgacg gtggtcgctt tgcctatccg ggttcgaacc
     1621 gctacgacgg acttgtgggg aagaccaagg tcttcaactt cccgctgccg ccgctggaca
     1681 acgagatcat cgagcgtcac tcggatccgg cgattcccgc actcgctgcg gcggccggca
     1741 tcaccgtgat ctcgggctac atcgtgcccc gcgtcttcgg ccgcgccgag gtcttcgaag
     1801 cgcaacgcat ccgcgaactg cgcatggcga tgcgcttgat gctgatggcg cccgcgaccg
     1861 attaccggca tccgcccggt ttgccgtcga tgccgaatct cgccacggac gcgccgccct
     1921 gcatgctgtc gagcggttcc ttcgggggcc tcacggcacc cgcgctctgt gtgcgctacc
     1981 ccaaggagtt ctgcgccgcc acggtcgagg tcttcggccc gtcaccacgc cgcgtgctcg
     2041 ccgaccagtt caacttcgaa tacctcgcca cgcgcaccgg cttctcgtcg tacggcggag
     2101 cctacagtcc gcacgacacg ctcgagtggg gcaccgcgtt ccgcttcatg gaccagaccc
     2161 cgggcagtcc gggttgggac tacttcaatc tctcaccgtt gatccgcaag cggcgcggcg
     2221 agctgtacgc ggcgatggcc tgggtctgtc aggacgagga caccatcggc cacggcaccg
     2281 actggatgtc cctgctgacc ggcacgcgca actacacgcc gcgattcgcg agcaccagcg
     2341 cgcctctctt gtacttcacc gccgtcgacc gccgctgcca cgagagtggc tggcacacca
     2401 cagcgatcgg cggtgtgggc ggcgcctggc tcggccgcag cgatgcgatc gagatggcgc
     2461 cggtggccta caccaactgg tcgaatcaga accccgtggt gccacccttg atccaggacg
     2521 acggcagcga ggacagctac gcctgggcgc tggaccgtgc gctccccgcc gactacgtcc
     2581 cgaaccccgg cgacaagctg ctgctcgaca ccagcttcgg ctcgcctgcg agtccgggtc
     2641 gcaccgaggg cgcaggcctc gcactcggtc tcgacgagtc gctgcgcttc gccgatgtgc
     2701 cgggagacgg cggtggcaag tcgatcttca ccggcagcgc cgatggcgtc gtgacccgct
     2761 tcgtgctcaa cagtgcgacc aaggccttcg acgtggctgc gcaatcggtg ccgctcggct
     2821 tcggtgcctt cgcgctcgcc gtgggcgatc tgtccgacgg ccacgccggt ctggaggtcg
     2881 ccgtcggcac caaggagcac ctcttcacgc tcgatgccct gaccctgagt gtgctgaact
     2941 ggcgcgaggt gccgcgcacc ttcgagcacg cacgtccgcg ccgcatgcag atcgccgatg
     3001 tgttcagcgg ccccgacttc cccggcaagg aggtgatcac gaccaccctg atggggcatg
     3061 tgctggtctt ctccggcgac ggcttcggcg atctggtcga ctttggcgag cctggagtcc
     3121 aggatctcgc ggtcctcgaa ggtcatgtga gcgcggcgtc caccaccacc tcgaacacgc
     3181 cgatcctgct gctctcgcat cgcggccacc tggcgcatgt gacgctcaac aaggtggcgg
     3241 atccgaccca gcgcaatccc ggcccggcag agctgcaggc ctggaccgct ggcgaggagg
     3301 ggacgcccat ggacctcgag atcgtgtcgg caccggtggg aggcgggccg gtcgcggtcg
     3361 cgctctatgc gcaccaggga accgacggaa ccaacgacgt cccgcagatc cgctgcttcg
     3421 atgccttgac catgcacccg acctcgatgg cgacccacgg cattccgccg agcctgggtt
     3481 ggttcgaact cggaaccctg gggcgcgatg tcctcgcggc cgacctcgcc ccggtgcatc
     3541 tcagcagcgg cgcgctcgcc ggcttcgtgg tgatgctgag tgcgcgcatc gtgtgggtgc
     3601 cgatcaacgg ctcccaaccc cagcagcctg caggttacaa gctcgacggc ttcgcgcccg
     3661 cttcgcgtgc catcgcggtg accaccgcgg acatcgccac gcgcagcggc ggcgagacct
     3721 acaaggagga gatcatcctc tccacgctcg gcggacatgt cgtctggttc cacctggagg
     3781 acatgctggc caacggtccg agcgtgtacc tcgacttcgt cggcaccccg ccgccgccac
     3841 cgattccact cggcgtgatc cccttcacca accgcaactc ggcagggcag tgggggctgc
     3901 tcgcccacga cgagggcaac ggcactcatc tcttcggcgc caaccagtcg ggtgggctct
     3961 acgagaccga tcccacgagc ggactgagcg tcatgcggga tgtgttccgg gaagccggca
     4021 acgtcgtggg aacatcgagt agggtgccca gcatcgtgac ggctcccgtc cgtgatctcg
     4081 tgcaaatcgg agcgggtgtt cagcagggcg caggcacgat cgaagcccgc acgtacacca
     4141 cgaccagcgc agggtctggt ctccgctggc tccagaccag accttggcaa gacgccatca
     4201 acgatgtcct gagaccggtt tggttccagg atcacacgga gatcaacggc atcgtctaca
     4261 acaccgaggc gcggatgccc gtgatcaacg gcttcgctgc cctgatcggc gcgggcgacg
     4321 ccatcgcgac gggttcgccg atcgcgggcg ccactcgtca gctccattgg tggggcggcg
     4381 atttcgacga cttcacgaat ctgatccagg gtgcctactt cgatgccacg cacgtgatcg
     4441 acaactggta ctcgacgagg aacgtgccgg ggggcgtgcc gccggtcccg ccgcagcaga
     4501 actggacggg tcgcaccagt ggcgactgca aggacctccg caacgagatt cgtcagtggg
     4561 ctcagatcaa caacatgcag tcgctgcgcc tcggcaccga tgccttcggc accgtggtcg
     4621 tggccagcac tgagggaggg agcgtggtct tgctccgtcc cggacgcgat gccgggcaag
     4681 cgggtcagga ctacggcacg atcctctggg acagcgccga tccgcagact cccgctgacg
     4741 agggcatgaa cggcatgggc ctcgccgtcc gcgcggtgcc caacgatccg agtcactccc
     4801 tcgacatctt cctcggtgtc ggggtcacgc atctcgatcc ccagcactgg ctcaatggca
     4861 gcggtccggg aaacctgact ggcggcatcg cctggtaccg gtggaccggc agttcagcga
     4921 ccagcggtac catggcccgc aacgggctgt tgcacctcga ttcgaactcg gggaacccca
     4981 tcggtgcccg cggcggcttc ggtgtgtgcg gtctggcgat cggcgatgtg atctccacgc
     5041 aggccggaga cgagctggtc gcgaccacgc tggagggcga cctcttcgtc ttcaccatac
     5101 ccaccagcgg gatgctgaac ccagggaaca tcctgcatcg ggcctgggca cgcggtgcgc
     5161 tgggcgtcaa caatggcatc gccatcttcg attcgaacaa cgacggcaag aaggagctct
     5221 tcatcgccgg ttcgctcggc atctggaagt ggaggcaacc atgagcctgc aactcgagac
     5281 ttcgatcgac tcgatctgcg tccgcgtgct tgagtgccta cttcggtggg cgcatcgcct
     5341 cgcgcttttc tgtggcatcg catggatcgc cgctgaggcg ctgcccgccc agcgcacctg
     5401 ggtggtcgat gacacgatgg ccgcggggac ggacttcccc gacttggtgt cggcctacgc
     5461 cgcggctgtt cctggcgatc ggatccacat ccgcttcggc agcgggcaag gctaccgggc
     5521 tcccgcatcg atcaacaagg cgctgaccat cgtcgggttg gggacgacca agcccattgt
     5581 ggtgcacagc ctggtcccga tctcgtgtcc cgcgcaacag gtcctggtgt tcgacaatct
     5641 ggatttccgt gccgacgcga atgggctcgc gacgagcgtc gagaacagcc tgggcaccgt
     5701 gttgctgtca cggatcagtt atccgggcgc gttgtacgat ccggtcaata cctggaacgc
     5761 gcatcgcgtt gtgatggtcg actgcgtggt gacggctcca gtccaggctg tggctgtgca
     5821 gggctccaaa gtctggttgc tgaactgcgt gacccgagcg cgatccgccg gtgatcccaa
     5881 ctacaccggc gacatccaga tcaggtacgg cggcttcctc accgtggtgg gcggtcaaca
     5941 ccaaggcg
//
LOCUS       DAOQNN010000183         5935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7741_length_5935_cov_11.357653, whole genome shotgun sequence.
ACCESSION   DAOQNN010000183 DAOQNN010000000
VERSION     DAOQNN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7741_length_5935_cov_11.357653"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1529
                     /locus_tag="PKV97_10370"
     CDS             <1..1529
                     /locus_tag="PKV97_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNB06322.1"
                     /translation="AADFYTAGGGITGFQNGLAIAGDYMSAASFLGISAAVMASGYDG
                     LIYSIGFLVGWPVITFLMAERLRNLGKFTFADVAAYRFQQTPIRAFAASGTLVVVAFY
                     LIAQMVGAGQLIKLLFGLEYWMAVVIVGALMMVYVLFGGMTATTWVQIIKACLLLAGA
                     SFMAFMVLLHYGFSPEAMFADAVRIKTEGAIAKGTVAEDAAKLGQSIMGPGSFIKDPI
                     SAISFGMALMFGTAGLPHVLMRFFTVPDAKEARKSVFWATTWIGYFYILTFIIGFGAI
                     VMVSTNPGFLDAKGGLIGGSNMAAIHLANAVGGNVFLGFISAVAFATILAVVAGLTLS
                     GASAVSHDLYATVFKKGNADSASELKVSRITTMVLGVIAVVLGIAFEKQNIAFMVSLA
                     FAIAASANFPVLFMSVLWKDCTTRGATIGGFIGLIVAVALTIVSPSVWEATLGNPKGS
                     ALFPYASPALFSMAAGFIGIWLFSILDNSARAKADRAGYMAQKVRSETGIGAASASAH
                     "
     gene            complement(1605..3047)
                     /locus_tag="PKV97_10375"
     CDS             complement(1605..3047)
                     /locus_tag="PKV97_10375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNB06323.1"
                     /translation="MQTSLRRKLLSSLAAPTGLLLAIAGAVAYGTAKGMVGDAYDHNL
                     LNLAHAIATHVHPEADGLNLRLSSEVEAVLRSDTQDRIYFRVRDLGGRLLGGDAALPL
                     LDDSDAASALPVPYSPVGRETPQPPRAPSTRPLFRDLVHAGEPIRALRLYRETPAGGY
                     YVTVAETLVKREAAMDRLLLGFVSAGALLLLAAGIAAGLGIPSGLAPLERLEESLRRR
                     AGADLSPIDPKAVPDEVREVVRALNGLLERQRNASARQREFLQDAAHQLRTPLAGLQM
                     QLELLESRPLDEAARHRLRVSVARVIRLANQLLALARAEAGGRLVADASEVELTALID
                     PLVEDWIERADARAIDLGVEREAVRMVGDPTLLQELIANLMDNALKYCDEGGKVSLRC
                     AREEGQVCIEVDDDGPGIPREVREQVFERFYRHAGTRASGSGLGLSIAREIVRGHGGS
                     ITIDDGPDGRGTRVRVMLPTDGHHGKTAGR"
     gene            complement(3025..3702)
                     /locus_tag="PKV97_10380"
     CDS             complement(3025..3702)
                     /locus_tag="PKV97_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB06324.1"
                     /translation="MRLLLVEDDPALAEGIAEFLRGQGDEVEVEGDGMRADRLLQNNG
                     YDFVLLDVGLPGLGGYELVRRIRKRGQRMPVIVITARDALDDRIYGLDLGADDYLVKP
                     FELAELSARMRAVQRRGGGAGARLAFGALALDLDGRLAELDGAPMTLSGREWEVLEAL
                     VRADGRTVTRENLQGDGSGNALEVCISRLRPRVEAAGLLIRTVRGFGYRLERSKEQRD
                     GADQPPA"
     gene            3877..5139
                     /locus_tag="PKV97_10385"
     CDS             3877..5139
                     /locus_tag="PKV97_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNB06325.1"
                     /translation="MSDMLPLDAEQIEAFLIATGIGLLIGLERERVPSARAGLRTFGL
                     VGMLGALVSMLGEQLGSPAPFVAGLVLVGLTIIAAYLRHPDSSDPGTTSVAALVLCYC
                     LGAASWLGHAQLAVMLAVGATVLLYFKAQLRGVATRLEQRDWISILQFGVLSLVILPM
                     LPDEEMGPHGALNPRQIWWMVVLIAGVGLAGYAALKIAGVRYGAAFVGVFGGLASSTA
                     TTLLYARQARELPAAGAMAALVIVIANLVMVLRVWGIAAVVAPGVAAPLLKLIVPALV
                     LGTLGALWLWRRLAERHETVLPATGNPTELKTALGFGAAYALVLLAAAWLSDYAGNRG
                     LYVLAFVSGLTDVDAITLSSLRLYALERLELVATVTALGIAMLANLCFKLGLASVIGG
                     RALALRAAAGMASVALGLLAGLAWVGLG"
     gene            complement(5174..5341)
                     /locus_tag="PKV97_10390"
     CDS             complement(5174..5341)
                     /locus_tag="PKV97_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06326.1"
                     /translation="MDKERLFRTGFAAFWLITLGLLVLDTMSSSRIRVEPPPIALGSG
                     QAASGGHCSGR"
     gene            complement(5352..>5935)
                     /locus_tag="PKV97_10395"
     CDS             complement(5352..>5935)
                     /locus_tag="PKV97_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNB06327.1"
                     /translation="DAPPSPQAVAEQLVRTGTASFVAATRPAQSRWATRWWLGALLLA
                     VLYLAHLVVAGQPWGIVYGMGVWGAKAVSTLGWDLSGDAFWGVAPHAQRLVEPILADV
                     TSVTNIGLIYGAIAASRWNGAPKFSAPSGRRLAAAAVAGLVMGYSARMAFGCNVGAYL
                     GGIASASLHGWVWFALAFAGSILGVRIRRRVGA"
BASE COUNT          780 a         2095 c         2099 g          961 t
ORIGIN      
        1 cggcggccga cttctacacc gccggcggtg gcatcaccgg cttccagaac ggcctcgcga
       61 tcgccggcga ctacatgtcg gcggcctcct tcctcgggat ctccgcggcg gtgatggcga
      121 gcggctacga cggcctgatc tactcgatcg gcttcctggt cggctggccg gtgatcacct
      181 tcctgatggc ggagcgcctg cgcaacctcg gcaagttcac cttcgccgac gtggcggcct
      241 accgcttcca gcagaccccg atccgcgcct tcgcggcctc gggcaccctg gtcgtggtgg
      301 ccttctatct gatcgcgcag atggtcggcg ccggccagct catcaagctg ctgttcggcc
      361 tggagtactg gatggcggtg gtcatcgtcg gcgcgctgat gatggtctac gtgctcttcg
      421 gcggcatgac ggcgaccacc tgggtgcaga tcatcaaggc ctgcctgctg ctcgcaggtg
      481 ccagcttcat ggccttcatg gtgctgctgc actacggctt ctcgcccgag gcgatgttcg
      541 ccgacgcggt gcgcatcaag accgagggcg ccatcgccaa gggcacggtg gccgaggatg
      601 ccgccaagct cggccagtcg atcatgggcc cgggctcctt catcaaggac ccgatctcgg
      661 cgatctcctt cggcatggcg ctgatgttcg gcaccgccgg cctgccgcac gtgctgatgc
      721 gcttcttcac cgtgccggac gccaaggaag cccgcaagtc ggtgttctgg gcgaccacct
      781 ggatcggcta cttctacatc ctgaccttca tcatcggctt cggcgcgatc gtgatggtgt
      841 cgaccaaccc gggcttcctg gatgccaagg gcgggctgat cggcggcagc aacatggcgg
      901 cgatccacct cgccaacgcg gtcggcggca acgtgttcct cggcttcatc tcggcggtgg
      961 cgttcgcgac catcctcgcg gtggtggccg gcctgaccct gtcgggtgca tcggcggtgt
     1021 cgcacgacct ctacgccacg gtgttcaaga agggcaacgc cgactcggct tccgagctca
     1081 aggtctcgcg catcaccacc atggtgctcg gcgtgatcgc ggtggtgctg ggcatcgcct
     1141 tcgagaagca gaacatcgcc ttcatggtgt cgctggcctt cgcgatcgcc gcctcggcca
     1201 acttcccggt gctgttcatg tcggtgctgt ggaaggactg cacgacccgc ggcgcgacca
     1261 tcggcggctt catcggcctg atcgttgcgg tagcgctgac catcgtgtcg ccctcggtgt
     1321 gggaagccac gctcggcaac ccgaagggtt cggcgctgtt cccctacgct tcgccggccc
     1381 tgttctcgat ggccgcgggc ttcatcggca tctggctgtt ctcgatcctc gacaacagcg
     1441 cgcgtgccaa ggccgaccgt gccggctaca tggcgcagaa ggtgcgttcg gagaccggta
     1501 tcggcgcggc gtcggcctcg gcgcactaag cagcaagcgc tccgccggca tccgcgaggg
     1561 tgccggcggg cagcacgcaa gaccgcgggg cggtgggcgc aggctcaccg ccccgcggtt
     1621 tttccgtgat ggccgtcggt cggcagcatt acccgcacgc gggtgccgcg cccgtccggg
     1681 ccgtcgtcga tcgtgatgct gccgccgtgg ccgcgcacga tctcgcgcgc gatcgacaga
     1741 cccaggccgc tgccgctggc gcgggtgccg gcgtggcggt agaagcgctc gaagacctgt
     1801 tcgcgcactt cgcgggggat gcccggaccg tcgtcgtcga cctcgatgca cacctggccc
     1861 tcttcgcgcg cgcagcgcag cgagaccttg ccgccttcgt cgcagtactt gagggcgttg
     1921 tccatcaggt tggcgatcag ctcctgcaga agggtggggt cgccgaccat gcgcaccgcc
     1981 tcgcgctcga cgccgagatc gatcgcgcgg gcgtcggcac gctcgatcca gtcctcgacc
     2041 agggggtcga tcagcgcggt cagctccact tccgacgcat ccgcgaccag gcggccgcct
     2101 gcttctgcgc gggcaagcgc gagcaactgg ttggcgagcc ggatcacccg cgccaccgag
     2161 acgcgaaggc ggtgccgcgc ggcctcgtcg agcgggcgcg actcgagcag ctcgagctgc
     2221 atctgcaggc cggcgagcgg ggtgcgcagc tggtgcgcgg catcctgcag gaactcgcgc
     2281 tggcgggcgc tggcattgcg ctggcgctcg agcaggccgt tgagtgcgcg caccacctcg
     2341 cgcacctcgt cgggaacggc cttggggtcg atcggcgaca ggtcggcgcc cgcgcgccgg
     2401 cgcagcgact cctccaggcg ctcgagcggg gcgagaccgc tcggaatgcc cagtccggcg
     2461 gcgatgccgg cggcgagcag gagcagggcg ccggcggaga cgaagccgag gagtagtcgg
     2521 tccatcgccg cctcgcgctt gaccagggtc tcggcgacgg tgacgtagta gccgcccgcg
     2581 ggtgtttcgc gatacaggcg caaggcgcgg atcggctcgc ccgcatggac gagatcgcgg
     2641 aacagcggtc gcgtggaggg cgcgcgcggc ggttgcggtg tctcgcggcc gaccggggag
     2701 tagggcaccg gcaaagcgga tgccgcatcg ctgtcgtcga gcagcggcag ggccgcgtcg
     2761 ccgccgagca ggcgcccgcc caggtcgcgt acgcggaagt agatgcggtc ctgggtgtcg
     2821 ctgcgcagca ccgcctcgac ttcggacgac agcctcaggt tgagcccgtc cgcttcgggg
     2881 tgcacatggg tggcgatcgc gtgggcgagg ttgagcaggt tgtggtcgta ggcatcgccg
     2941 accatgccct tggccgtgcc gtaggccacc gcgccggcga tcgccagcag caggccggtg
     3001 ggtgcggcga ggctgctgag gagcttacgc cggaggctgg tctgcaccgt cgcgctgctc
     3061 cttgctgcgt tcgaggcggt agccgaagcc gcgtacggtg cggatcagca accctgccgc
     3121 ctccacgcgc gggcgcaggc gcgagatgca cacctcgagc gcgttgccgc tgccgtcgcc
     3181 ttgcaggttc tcgcgcgtca ccgtgcgccc gtcggcgcgc accagcgcct ccagcacctc
     3241 ccactcgcgc ccgctgagcg tcatcggcgc gccgtcgagc tcggcgaggc ggccgtcgag
     3301 gtcgagggcc agcgccccga aggccagcct cgccccggcg ccacccccgc gccgctgcac
     3361 cgcgcgcatg cgcgcactga gctcggcgag ctcgaagggc ttcaccaggt agtcgtcggc
     3421 gccgaggtcg agcccgtaga tgcggtcgtc gagcgcgtcg cgcgcggtga tcacgatcac
     3481 cggcatgcgc tggccgcgct tgcggatgcg acgcaccagc tcgtagccgc cgagcccggg
     3541 caggccgacg tcgagcagga cgaagtcgta gccgttgttc tgcagcaggc ggtctgcacg
     3601 catgccgtcg ccttccacct cgacctcgtc gccctggccg cgcaggaatt ccgcgatgcc
     3661 ctccgcgagc gcggggtcgt cctcgaccag caaaagtctc atctgtgctt ctccatgccg
     3721 cgccgcgcgg gcggaccccg cgtccatcgc gcagccgcga gcgtcgtgtg gagcgaatcg
     3781 tatcccggca cgggcgtgct tgcatcgccg cgcacgccct ggcgtatgat cgggctgtcg
     3841 atcgccctct gccggggccg cacccggccc gccgccatgt cggacatgct gccgctcgat
     3901 gccgaacaga tcgaagcctt cctgatcgcg accggaatcg gcctgctgat cggcttggag
     3961 cgcgagcgtg tgccctcggc gcgtgccggg ctgcgcacct tcggcctggt cggcatgctc
     4021 ggcgcgctgg tgtccatgct cggcgaacag ctcggcagcc ccgcgccctt cgtcgccggc
     4081 ttggtgctgg tgggcctcac catcatcgcc gcctacctgc gccaccccga ttcttccgat
     4141 cccggcacca cctcggtggc ggcgctggtg ctgtgctact gcctgggcgc cgcgagttgg
     4201 ctcggccacg cgcagctcgc ggtgatgctt gcggtggggg ccaccgtgct gctctatttc
     4261 aaggcacaac tccgcggcgt ggcgacgcgg ctggaacaac gcgactggat ctcgatcctg
     4321 cagttcggcg tgctctcgct ggtcatcctg ccgatgctgc cggacgagga gatgggcccc
     4381 cacggcgcgc tcaatccgcg ccagatctgg tggatggtgg tgctgatcgc cggtgtcggc
     4441 ctggccggct atgcggcgct gaaaatcgcc ggcgtgcgct acggtgccgc cttcgtcggt
     4501 gtcttcggcg gcctggcatc gagcacggcg accacgctgc tgtacgcgcg tcaggcgcgc
     4561 gagctgcccg cggcgggtgc gatggccgcg ctcgtgatcg tgatcgccaa cctggtgatg
     4621 gtgttgcggg tatgggggat cgccgccgtg gtcgcgcccg gcgtcgccgc gccgctgctg
     4681 aagctgatcg tgccggcgct cgtgctcggc acccttggtg cgctgtggct gtggcgccgg
     4741 ctcgccgaac gtcacgagac cgtgctgccc gccaccggca acccgaccga actgaagacc
     4801 gcgctcggtt tcggtgcggc ctatgcgctg gtgctgctgg ccgcggcctg gctgtcggac
     4861 tatgcgggca atcgcggctt gtacgtgctg gccttcgtct ccggcctcac cgacgtcgat
     4921 gcgatcaccc tgtccagcct gcgcctgtac gcgctcgagc gcctcgaact ggtcgccacg
     4981 gtcaccgcgc tcggcatcgc catgctcgcc aacctctgct tcaagctcgg cctcgccagc
     5041 gtgatcgggg gccgcgccct cgcgctacgt gccgccgccg gcatggccag cgtcgcgctc
     5101 ggcctgctcg ccggcctggc ctgggtggga ctcgggtaag gcgcgtccgg ctttcgcgcc
     5161 ttaggccgca cgcctaacgt cccgagcaat gcccccccga ggcggcctgg ccgctgccga
     5221 gcgcgatcgg cggcggctcc acgcggatcc tgctgctgct catggtgtcg agcaccagca
     5281 agccgagggt gatcagccag aaggccgcga agccggtgcg gaagagtctt tccttatcca
     5341 tgtcttcctc ctcacgcacc gacgcggcgg cggatgcgca caccgaggat ggagccggca
     5401 aaggcgagcg cgaaccacac ccagccgtgc aggctggccg aggcgatgcc gcccaggtag
     5461 gcgccgacgt tgcatccgaa ggccatgcgt gcgctgtagc ccatcaccag cccggccacc
     5521 gcggcggcgg cgaggcggcg cccggagggc gcggagaact tcggcgcgcc gttccagcgc
     5581 gaggcggcga tcgcgccgta gatcaggccg atgttggtca ccgaggtgac gtcggcgagg
     5641 atgggctcga ccaggcgctg cgcatgcggc gccacgcccc agaaggcgtc gcccgagagg
     5701 tcccagccga gcgtggagac cgccttcgcg ccccacacgc ccatgccata cacgatgccc
     5761 cagggctggc ctgcgacgac cagatgggcg aggtagagca cggccaggag cagcgcgccg
     5821 agccaccagc gcgtggccca gcgcgactgc gcgggcctcg tggcggcgac gaagctcgct
     5881 gtgccggtgc gcaccagctg ttcggcgacg gcctgcggcg agggcggtgc gtcgc
//
LOCUS       DAOQNN010000184         5935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7742_length_5935_cov_10.143707, whole genome shotgun sequence.
ACCESSION   DAOQNN010000184 DAOQNN010000000
VERSION     DAOQNN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7742_length_5935_cov_10.143707"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKV97_10400"
     CDS             <1..247
                     /locus_tag="PKV97_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HNB06328.1"
                     /translation="LPAVTRQPGQADAGTVWVVGEDDAVRPLTVELGAWREDGVVVRA
                     GLPAQARVVVAGVYKLIEGMRVRAVEEGAPVLLDVRR"
     gene            244..3465
                     /locus_tag="PKV97_10405"
     CDS             244..3465
                     /locus_tag="PKV97_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB06329.1"
                     /translation="MSMKGEGPIGSGRFNLSEWGLTHRSLVLYFLIVCTLSGMFAYTR
                     LGQSEDPPFTFKVMVVRAEWPGASAREVEQQVTDKIEKKLQEIAQADIIRSYSKPGEA
                     MVFFVAKDSTRARDIPDIWYQARKKIGDIAHTFPTGVRGPYFNDEFGDTYGNVFALLG
                     EGVSHAELERHAEAIRGELLRVKDVAKVSFFAEQPQRVFVEISNTRLATLGVRLDDIV
                     GALARQNAETGAGFFETGDERIWLRPSGQFEDLDAIRATVIRAGGRSLRLGDIAEVRR
                     GYADPPAERLRFMGQEALGIGVSMRSGGDIVALGRQLDAAVEKIVAQLPVGIRLERVA
                     DQPRAVTRAVDEFVKVLAEAVLIVMAVSLLSLGFRTGVVVLVIIPVVLAITFMFMHLF
                     DIGLHKISLGALIIALGLLVDDAIIAVEMMASKMEQGWGRVRAASYAWTSTAMPMLSG
                     TLVTAAGFLPVALAASSVGEFTRSIFQVTVTALLVSWIAAVLFVPYLGYYLLPDFSKR
                     AGQPLRLARVVGRVWPALGRRMAERDLHAHEQHDEFAIYHTPFYNRLRALVNACVRWR
                     WGVILLTVAIFVGALFVFARFVPQQFFPDSTRPELLVDLRLAEGASHHATEVAVKRLE
                     AFLDRQEGIENYVAWVGTGSPRFYLPLDQQLAQTNFAQLVILTAGLAEREALRAKLIH
                     LFEDEPWPLRGIVVRLENGPPVGFPVQYRVSGPDLAELHRLAQAVAEAMRANPHLSNV
                     HMDWVEPSKVVRVAIDQDKARLLGVSTQDLSSLLATSLQGMTATQFREGNKTIALVLR
                     GAESERAHLSQLPDLALPVGGGRSVPLAQVARLEHDFEPGVIWRRNRIPTVTVRASLY
                     DGTQPAVVVGQLAPRMAELSAQLPLGYRLEVGGAVEESAKGSGSVGAGMPIFVLTVLT
                     VLMLQLKSFSRTIIVVLTAPLGLIGVAAFLLAFGKPFGFVALLGTIALSGMIMRNSVI
                     LVDQIRQDVEAGRMQWEAIVESAVRRFRPIMLTAAAAILAMVPLSRSTFFGPMAVAIM
                     GGLTVATVLTILFLPALYAAWYRVKPADEERAATDRSAATALE"
     gene            3532..4185
                     /locus_tag="PKV97_10410"
     CDS             3532..4185
                     /locus_tag="PKV97_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNB06330.1"
                     /translation="MNFEKARFNMVEQQIRPWEVLDQDVLDLLMTVKREEFVPAAHRE
                     LAFADVEIPIGCGQVMLKPVIEGKILQALRVTKSDTVLEIGAGSGWFAALLAARAEWV
                     RTMEIEPELVKLASANLERNGVHNVVVVDGDGVQGWSGRAPYDVIVVSGGLPFVPQSL
                     LEQLKVGGRLFAFVGEAPVMKARLITCEGEGRFRTEDVFETVVPMLKNAPQREAFSF"
     gene            4193..4516
                     /locus_tag="PKV97_10415"
     CDS             4193..4516
                     /locus_tag="PKV97_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB06331.1"
                     /translation="MLQITPTMLAERLQDARRPAPLLLDVREPWEVQICQIPGSVTMP
                     MGSVPARYAELERDRETVVICHHGGRSWQVAMFLERQGFSNVANLSGGVAGWANEVDR
                     NMPQY"
     gene            4545..5849
                     /locus_tag="PKV97_10420"
     CDS             4545..5849
                     /locus_tag="PKV97_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNB06332.1"
                     /translation="MKRVLAVLVAGLFAGAAAAADLMQVYQDALANDAKFSAARAQLE
                     AGQEKVVQGRAGLLPQVGMDANTTRNDAKLKPVGASPREGSYNSNGYGVQLTQPLFRW
                     QNWVQFKQGELQTALAETQFGIARQDLVLRVAEAYFNVLNAQDALAAVTQLRTAAGEQ
                     LELAKTSFEVGTVTITDVHEAQSRFDLASAQEIAAQNLLEVARQTLAQIIGKQPEALA
                     GLREGVALQRPQPENIVDWVAAAESGGFGVQAQQLVREIAVREVERARAGHLPTVDVV
                     ARHGVNNDLQLSAGRNEVSSVGLQLNVPLFAGGRVSSVSREAAALRMKADADLEDARR
                     SAALAARQSWLGVTSGMAQVKALEAARVSSTSALDANKLGYEVGVRINIDVLNAQTQL
                     ADTLQKLARARYDTLLAQLRLKAAAGTLGEDDVRAINALLAP"
BASE COUNT          863 a         2017 c         2119 g          936 t
ORIGIN      
        1 gctgcccgcg gtgacgcgac aacccggcca ggccgatgcc ggcacggtgt gggtggtggg
       61 cgaggacgac gccgtgcggc cgctcacggt cgagctcggc gcctggcgcg aggacggcgt
      121 cgtggtgcgc gccggcctgc cggcgcaggc ccgtgtcgtg gtggccgggg tgtacaagct
      181 gatcgagggc atgcgcgtgc gtgccgtcga ggaaggcgcg ccggtgctgc tggacgtgcg
      241 gcgatgagca tgaagggcga ggggccgatc ggcagcgggc gcttcaacct gtccgaatgg
      301 gggctgaccc accgctccct ggtgctgtac ttcctgatcg tgtgcacgct gagcgggatg
      361 ttcgcctaca cccggctcgg ccagtccgag gatccgccct tcaccttcaa ggtcatggtg
      421 gtgcgcgccg agtggccggg ggcgagtgcg cgcgaggtcg aacagcaggt caccgacaag
      481 atcgagaaga agctgcagga gatcgcccag gccgacatca tccgcagcta ctccaagccg
      541 ggcgaggcga tggtcttctt cgtggcgaag gactccacgc gcgcgcgcga catccccgac
      601 atctggtacc aggcgcgcaa gaagatcggc gacatcgccc acaccttccc gaccggcgtg
      661 cgcggaccgt atttcaacga cgagttcggc gacacttacg ggaatgtctt cgccctgctc
      721 ggcgagggcg tgagccacgc cgagctcgag cgtcacgccg aggcgatccg cggcgagctg
      781 ctgcgcgtga aggacgtcgc caaggtgagc ttcttcgccg agcagccgca gcgcgtcttc
      841 gtggagatct ccaacacgcg cctcgccacc ctgggcgtgc gcctggacga catcgtcggc
      901 gcgctcgccc ggcagaacgc ggagaccggc gccggcttct tcgagaccgg ggatgagcgc
      961 atctggctgc ggccgtcggg gcagttcgag gacctagacg cgattcgcgc caccgtgatc
     1021 cgcgccggcg ggcgcagcct gcgcctgggc gacatcgccg aggtgcgccg cggctacgcc
     1081 gacccgccgg ccgagcgcct gcgcttcatg gggcaggagg cgctcggcat cggtgtgtcg
     1141 atgcgcagcg gcggcgacat cgtcgcgctc ggccgccagc tcgacgcggc ggtggagaag
     1201 atcgtcgcgc agttgcccgt cggcatccgc ctcgagcgcg tggccgacca gccgcgtgcg
     1261 gtcacgcggg cggtcgacga gttcgtcaag gtgctcgccg aggcggtgct catcgtgatg
     1321 gcggtgagcc tgctgtcgct cggcttccgc accggcgtgg tggtgctggt gatcatcccg
     1381 gtggtgctgg cgatcacctt catgttcatg cacctgttcg acatcggcct gcacaagatc
     1441 tcgctcgggg cgttgatcat cgcgctcggc ttgttggtgg acgacgcgat catcgccgtc
     1501 gagatgatgg cgagcaagat ggagcagggc tggggccggg tgcgggcggc gagctacgcg
     1561 tggacctcga ccgcgatgcc gatgctctcg ggcacgctgg tgacggcagc gggtttcctg
     1621 ccggtggcgc tggcggcgtc ctcggtgggc gagttcacgc gctcgatctt ccaggtcacg
     1681 gtgaccgcgc tgctggtgtc gtggatcgcc gcggtgctct tcgtgcccta tctgggctac
     1741 tacctgctgc ccgacttcag caagcgcgcc gggcagccct tgcggctggc gcgcgtggtc
     1801 ggaagggtct ggccggcgct ggggcggcgg atggcggagc gcgacctcca tgcccacgag
     1861 cagcacgacg agttcgcgat ctaccacacg cccttctaca accggctgcg ggcgctggtg
     1921 aacgcctgcg tgcgctggcg ctggggggtg atcctgctca ccgtggcgat cttcgtcggc
     1981 gcgctgttcg tgttcgcccg cttcgtgccg cagcagttct tccccgactc gacgcggccc
     2041 gagctgctgg tcgacctgcg cctggccgag ggtgcgtcgc accacgccac cgaggtcgcg
     2101 gtgaagcggc tggaggcctt cctcgacagg caggagggca tcgagaacta cgtggcctgg
     2161 gtgggcaccg gcagcccgcg cttctacctg ccgctcgacc agcagctcgc gcagaccaac
     2221 ttcgcccagc tcgtgatcct caccgccggc ctggccgagc gcgaggcgct gcgcgcgaaa
     2281 ctgatccacc tcttcgagga cgagccctgg ccgctgcgcg gcatcgtcgt ccggctggag
     2341 aacgggccgc cggtgggctt tccggtgcag taccgggtga gcgggccgga tctggccgag
     2401 ctgcaccggc ttgcgcaggc ggtggccgag gcgatgcgcg ccaatccgca cctttccaac
     2461 gtgcatatgg actgggtgga gccgtccaag gtggtgcggg tggcgatcga ccaggacaag
     2521 gcgcgcctgc tcggcgtgtc cacccaggac ctctccagtc tgctcgccac ctcgctgcag
     2581 ggcatgaccg caacccagtt ccgcgagggc aacaagacca tcgcgctggt gctgcgcggt
     2641 gcggagagcg agcgcgcgca cctgtcgcag ctgcccgacc tcgccctgcc ggtgggcggt
     2701 gggcgcagcg tgcccttggc gcaggtggcg cgcctcgagc acgacttcga gccgggggtg
     2761 atctggcggc gcaaccgcat ccccaccgtg accgtgcgcg cctcgctcta cgacggcacc
     2821 cagcccgcgg tggtggtggg ccagctcgcg ccgcgcatgg ccgagctctc cgcgcagctg
     2881 ccgctcggct accggctcga ggtcggcggc gcggtcgagg agagcgccaa gggcagcggc
     2941 tcggtgggcg ccggcatgcc gatcttcgtg ctcaccgtgc tcacggtgct gatgctgcag
     3001 ctgaagagct tctcgcgcac catcatcgtc gtgctcaccg caccgctcgg cctgatcggt
     3061 gtcgccgcct tcctgctcgc cttcggcaag cccttcggct tcgtcgccct gctcggcacg
     3121 atcgcgctgt cgggcatgat catgcgcaac tcggtgatcc tggtggacca gatccgccag
     3181 gacgtcgagg ccgggcgcat gcagtgggag gccatcgtcg agtccgcggt gcggcgcttc
     3241 cgcccgatca tgctcaccgc cgcggcggcc atcctggcca tggtgccctt gtcgcgcagc
     3301 accttcttcg ggccgatggc ggtggcgatc atgggcgggc tgaccgtcgc cacggtgctc
     3361 accatcctct tcctgcccgc gctctatgcc gcctggtacc gtgtcaagcc ggcggacgag
     3421 gagcgcgcgg cgacggatcg cagcgcagca actgcgctag aatgacgagg ttttgccttc
     3481 atggataacg ctatccatta gcgtgtgcta atatttcgcc gggagcccct gatgaacttc
     3541 gagaaagcgc gcttcaacat ggtcgagcag cagatccgcc cctgggaggt gctggatcag
     3601 gacgtgctcg acctcctgat gacggtcaag cgcgaggaat tcgttcccgc cgcgcatcgc
     3661 gagctggcct tcgccgacgt cgagatcccg atcggctgtg gccaggtcat gctcaagccg
     3721 gtgatcgaag gcaagatcct gcaggcgctg cgtgtcacca agtccgacac cgtgctcgag
     3781 atcggcgccg gctcgggctg gttcgccgcg ctgctggcgg cgcgtgccga gtgggtgcgc
     3841 accatggaga tcgagcccga gctggtgaag ctggcgagcg ccaacctcga gcgcaacggc
     3901 gtgcacaacg tggtcgtggt cgacggcgac ggtgtgcagg gatggtctgg ccgcgcgccc
     3961 tacgacgtga tcgtcgtttc cggcgggctg cccttcgtgc cgcagtcgct gctcgagcag
     4021 ctcaaggtgg gtggtcgcct gttcgccttc gtcggcgagg cgccggtcat gaaggcgcgc
     4081 ctgatcacct gcgagggcga aggccgcttc cgtaccgagg acgtgttcga gaccgtggtg
     4141 cccatgctca agaacgcgcc ccagcgcgaa gccttcagct tctgaggcga cgatgctgca
     4201 gatcacgccg accatgctcg ccgagcgcct gcaagacgcg cgacggcctg cacccctgct
     4261 gctcgacgtg cgcgagccgt gggaggtgca gatctgccag atccccggct cggtgacgat
     4321 gccgatgggc agcgtcccgg cgcgttacgc ggagctggaa cgcgaccgcg agacggtggt
     4381 catctgccat cacggcggac gcagctggca ggtcgcgatg ttcctcgagc gccagggctt
     4441 ctccaacgtg gccaacctgt ccggcggcgt cgccggctgg gcgaacgagg tcgatcgcaa
     4501 catgccgcag tattgagcgc ggcgcagcag cagcggagaa cacgatgaag cgagtcctgg
     4561 cagttctggt ggcgggtctg ttcgcgggcg ctgccgcggc ggccgacctg atgcaggtgt
     4621 accaggacgc gctcgccaac gacgccaagt tctccgccgc gcgcgcccag ctcgaggccg
     4681 ggcaggagaa ggtggtgcag ggccgcgccg ggctgctgcc gcaggtgggc atggacgcca
     4741 acaccacccg gaacgacgcc aagctcaagc cggtcggggc gtcgccccgt gaaggcagct
     4801 acaacagcaa cggctacggc gtgcagctta cccagccgct gttccgctgg cagaactggg
     4861 tgcagttcaa gcagggtgag ctgcagactg cgctcgccga gacccagttc ggcattgcac
     4921 gccaggatct cgtgctgcgc gtcgccgagg cctacttcaa cgtgctcaac gcccaggacg
     4981 cgctcgccgc ggtgacgcag ctgcgcaccg cggccggcga gcagctcgag ctggcgaaga
     5041 ccagcttcga ggtcggcacg gtgacgatca ccgacgtgca tgaggcgcag tcgcgcttcg
     5101 acctcgcctc ggcgcaggag atcgcggcgc agaacctgct cgaggtcgcg cgccagacct
     5161 tggcgcagat catcggcaaa caacccgagg cgctggccgg cctgcgcgaa ggcgtcgcgc
     5221 tgcagcgccc gcaacccgag aacatcgtcg actgggtggc cgcggccgaa tccggcggct
     5281 tcggcgtgca ggcccagcag ctggtgcgcg agatcgccgt gcgcgaagtc gaacgcgccc
     5341 gcgccggcca tctgcctacg gtcgatgtcg tcgcccggca cggtgtcaac aacgacctgc
     5401 aactctcggc cggacgcaat gaagtgagca gtgtcggcct gcagctcaac gtgcccttgt
     5461 ttgcgggcgg ccgggtgtcc tcggtgtcgc gcgaggccgc tgcgctgcgc atgaaggccg
     5521 acgccgatct cgaggacgcc cgccgcagcg ccgcgctcgc cgcacgccag tcctggctgg
     5581 gcgtgaccag cggcatggcg caggtgaagg cgctcgaggc cgcgcgcgtc tcgtccacct
     5641 cggcgctcga cgccaacaag ctcggctacg aggtcggcgt gcgcatcaac atcgacgtgt
     5701 tgaacgccca gacccagctc gccgacacct tgcagaagct cgctcgtgcg cgctatgaca
     5761 ccctgctcgc gcagttgcgc ctgaaggccg ccgccggcac cctgggcgag gacgacgtgc
     5821 gcgcgatcaa cgcactgctc gcgccctgaa cgtcccggca cggctcacgc ctcacgcctc
     5881 gcggccagcg ccgtgagcag cgcaacgctg cgcgcggtcg cgccttggtg cgtgg
//
LOCUS       DAOQNN010000185         5925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7769_length_5925_cov_9.534923, whole genome shotgun sequence.
ACCESSION   DAOQNN010000185 DAOQNN010000000
VERSION     DAOQNN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7769_length_5925_cov_9.534923"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1742
                     /locus_tag="PKV97_10425"
     CDS             <1..1742
                     /locus_tag="PKV97_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB06333.1"
                     /translation="DIRARAEALPKKRRTPLVQGFIEHFARYRYTVGPTIAFLQGRDP
                     TIAHRIASRSFLPEGPRFDTLDAYVDPDDPEGGDPLAWAFGALGLADRARHLATLYLN
                     ELADILRDAVDARFEFVRYAETLAMSQPTFEPLAAALAAVPTLVDAYLKDLTLEALCR
                     HSPQVVLVSTPFPGSVYGAFRIAQAIKAAHPEIVTVLGGGFVNTELRELAEPRVFDHF
                     DYVTLDDGERPLLALLEHLQGRRGRSRLVRTFVRDPDTGQVRYIHLAEPDVPFAEVGT
                     PTWDGLPLDGYLSVLDMLNPMHRLWSDGRWNKLTVAHGCYWKKCSFCDVSLDYIGRYD
                     GAAAGLLVDRIEAIIAETGQTGFHFVDEAAPPKALRALAEELLRRGVAISWWGNIRFE
                     KSFSPELCQLLADSGCIAVSGGLEVASDRLLQLMKKGVSVEQVARVTHAFTAAGVLVH
                     AYLMYGFPTQTVHDTVDALEYVRQLFEAGCIQSGFFHRFACTVHSPVGRNPEEFGVTL
                     VALPEITFAKNDVGFIDPTGVDHDRLGKALNKALYNYMHGIGLDTDVRAWFEDKVPRP
                     RVARHFIARALGA"
     gene            1869..3566
                     /locus_tag="PKV97_10430"
     CDS             1869..3566
                     /locus_tag="PKV97_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNB06334.1"
                     /translation="MDKQPTEGPELEVSFDELTRVLSVSIVHDPHFPRIDALWLRRRL
                     EAAGYADLQIRPDPIRRLIAQYNAGEAVAPVEIAQCVDASMQIGVSLDGLVARLSIVP
                     AKGGKPASKTELLALIESRGIVEGLLLEEMNRAIADGQADDLAIARGREPEPGKDGWL
                     EYLLPETRERVPSVRPCGRTDYRDLGEILVVHAGDALMRRHPPQPGVDGVNVYGRPIV
                     ARRGREQRFAPGLRGTATSPEDPELLVAACDGQPVRVRNGAMVEPIFTVDAVNLATGN
                     IDFDGSVRIRNDVQAGMTVRASGDIEVGGVVEPATLEAGGSIVVKGGVLGGLGGKTAG
                     KDYSAHAIRCEGSFCATYAQQARISAGDSIFIDDVAMQCQLEARNHIRVGKRLRGQIV
                     GGHCRASLSIHARTIGANSRIRTELEVGMDNGLEHAIQEKAEARDALENRLLEIGKML
                     TFADRHPDRVTPEMLGRAEQTASALSGEIESLRSEEEDLQHRLALTREARVNAEREMF
                     EGCIVRMGEQLFKLSQDRGPTTVRLATQGLGVFPLEDDSRFDEPQRPAAASQGSSRR"
     gene            complement(3610..4110)
                     /gene="tpx"
                     /locus_tag="PKV97_10435"
     CDS             complement(3610..4110)
                     /gene="tpx"
                     /locus_tag="PKV97_10435"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNB06335.1"
                     /translation="MSKVTLGGNPIDVAGRFPQAGEAAPAFRLTGADLADVGLEAFAG
                     KRKVLNIVPSLDTPVCATSTRKFNAEAAGLADTVVLVVSADLPFAAKRFCETEGLKNV
                     HTLSTFRNPKFAQDYGVAITSGPLAGVTARAVVVVDGNDKVVYSQLVPEIKEEPDYAA
                     ALAALK"
     gene            4353..5504
                     /locus_tag="PKV97_10440"
     CDS             4353..5504
                     /locus_tag="PKV97_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNB06336.1"
                     /translation="MEPVDLFYSYAHEDEKLRDELDGHLALLRRKGVIRPWHDRGIVP
                     GQEWDKTIDEQLAMADLILLLVSMDFLNSDYIWGKELATAIERARRGDASVIPVLLRA
                     VDLEDAPFAHLQGLPTDLRPVTSWPNRDEAWTDVAKGIRRAVEAIRQRWASAPPPAPP
                     PAAPPETPSVPRASGFGTAVASVPLPEEPVRTLEMARPAPRMAATRDARTDALLERTI
                     AAFANELALAARIKGVQGIGSGEAEGLAEALIDMPAQKRVLWVDDRPENNRHELAALA
                     KLQVEVVNVRSTGEAIACLDADEEGFDLILSDWDRAEPAATASSAGLELLRMLAARPR
                     RPPVIFYHAEFDPSRRAARRECLLRAGAFGEAVRPGELLALIRSAFASD"
     gene            complement(5519..>5925)
                     /locus_tag="PKV97_10445"
     CDS             complement(5519..>5925)
                     /locus_tag="PKV97_10445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNB06337.1"
                     /translation="TISATIAGAISMLANFGMFFGGREGEERPNPIVSIAMMILAPLA
                     GMLIQMAISRTREFGADRGGAEISGDPAALAAALAKIDAYARGIPMHTAERHPETAQM
                     MIMNPLSGGGLRGLFSTHPSTQERIARLQTMR"
BASE COUNT          915 a         2109 c         2065 g          836 t
ORIGIN      
        1 acgacatccg cgcccgcgcc gaggcgctgc cgaagaagcg gcgcacgccg ctggtgcagg
       61 gcttcatcga gcacttcgcg cgctaccgct acaccgtcgg ccccaccatc gccttcctgc
      121 agggccgcga cccgaccatc gcgcaccgca tcgccagccg cagctttctg cccgaggggc
      181 cgcgcttcga caccctagac gcctatgtcg accccgacga ccccgagggc ggggacccgc
      241 tcgcctgggc cttcggcgcc ctcggcctcg ccgaccgcgc acgccacctg gcgacgctgt
      301 acctcaacga gctcgccgac atcctgcgcg acgcggtgga tgcgcgcttc gagttcgtgc
      361 gctacgccga gacgctggcg atgagccagc ccaccttcga gccgctcgcc gccgcgcttg
      421 ccgccgtacc gacgctggtc gacgcgtatt tgaaagacct gaccctcgaa gccctctgcc
      481 gccactcgcc gcaggtggtg ctggtctcca cccccttccc cggctcggtg tacggcgcct
      541 tccgcatcgc gcaggcgatc aaggccgcgc atccggagat cgtcaccgtg ctcggcggcg
      601 gcttcgtcaa taccgagctg cgcgagctcg ccgagccacg ggtgttcgac catttcgact
      661 acgtcacgct cgacgacggc gagcgcccgc tgctcgcgct gctcgagcac ctgcagggcc
      721 ggcgcggcag atcccggctg gtgcggacct tcgtgcgcga tccagatacg ggccaggtgc
      781 gctacatcca cctcgccgag cccgacgtgc ccttcgccga ggtcggcacc ccgacctggg
      841 acggcctgcc gctggacggc tacctgtcgg tgctcgacat gctcaacccg atgcaccgac
      901 tgtggtcgga cgggcgctgg aacaagctca ccgtggccca cggctgctac tggaagaagt
      961 gcagcttctg cgacgtcagc ctcgactata tcggccgcta cgacggcgcc gccgccggcc
     1021 tgctggtcga ccgcatcgag gcgatcatcg ccgagaccgg ccagaccggc ttccacttcg
     1081 tcgacgaggc cgcgccgccc aaggccttgc gtgcgctcgc cgaggagctg ctgcggcgcg
     1141 gcgtggcgat ctcgtggtgg ggcaacatcc gcttcgagaa atccttcagc cccgagctct
     1201 gccagctgct cgccgacagc ggctgcatcg cggtctcggg cgggctggag gtggcctccg
     1261 accgcctgct gcagctgatg aagaagggcg tttcggtcga gcaggtcgcg cgcgtcaccc
     1321 acgccttcac cgcggccggg gtgctggtgc acgcctacct gatgtacggc ttccccaccc
     1381 agacggtgca cgacaccgtc gacgcgctcg aatacgtgcg ccagctcttt gaagccggct
     1441 gcatccagtc gggcttcttc catcgtttcg catgcaccgt gcattctccg gtgggcagga
     1501 acccggagga gttcggcgtc accctggtgg cgctgcccga gatcacgttc gcgaagaacg
     1561 acgtcggctt catcgacccc accggggtgg accacgaccg cctcggcaag gcgctcaaca
     1621 aggcgctata caactatatg cacggcatcg gcctggacac cgacgtgcgc gcctggttcg
     1681 aggacaaggt gccgcgcccg cgcgtggcca ggcacttcat cgcccgcgca ctgggcgcct
     1741 gagcattgca cgggcgacac ggccgcgggc gtcgaatcgg gttcgcccca ttggcaccaa
     1801 caatatgcgc gcggctacaa tcccccgacc gcgcacgcgg accttgctga tcggatcgca
     1861 ctggaaacat ggacaagcag cccacggaag gcccggaact ggaggtgtcg ttcgacgaac
     1921 tcacgcgcgt cctcagcgtg agcatcgtcc atgacccgca ttttccgcgc atcgacgcct
     1981 tgtggctgcg ccgacggctc gaagccgcgg gctacgccga cctgcagatc cgccccgacc
     2041 cgatccgccg cctgatcgcc cagtacaacg ccggcgaggc ggtggcgccg gtcgagatcg
     2101 cgcagtgcgt ggatgcgtcg atgcagatcg gagtctcgct ggacggactg gtcgcacgcc
     2161 tgagcatcgt gccggccaag ggtggcaagc cggcgagcaa gaccgaactg ctcgccctga
     2221 tcgaatcgcg cggcatcgtc gagggcctgc tgctggagga gatgaaccgc gccatcgccg
     2281 acggtcaggc cgacgacctc gccatcgcgc gcggccggga accggaaccg ggcaaggacg
     2341 gctggctcga gtacctgctg ccggagacgc gcgagcgcgt acccagcgtg cgcccctgcg
     2401 gccgcaccga ctaccgcgac ctcggcgaga tcctcgtcgt ccacgccggc gatgcgctga
     2461 tgcgccgcca tcccccgcag cccggagtcg acggcgtcaa tgtgtacggc cggccgatcg
     2521 tggcgcggcg cggtcgcgag cagcgcttcg cccccggcct gcgcggcacc gcgacctcgc
     2581 ccgaggaccc cgagctgctc gtggctgcct gcgacggcca gccggtgcgg gtgcgcaacg
     2641 gcgcgatggt ggagccgatc ttcaccgtgg atgcggtcaa cctcgccacc ggcaacatcg
     2701 acttcgacgg cagcgtgcgc atccgcaacg atgtgcaggc cgggatgacg gtgcgcgcca
     2761 gcggcgacat cgaggtcggc ggcgtggtcg agccggccac gctggaggcc ggcggcagca
     2821 tcgtggtcaa gggcggcgtg ctcggcgggc tgggcggcaa gaccgccggc aaggattaca
     2881 gcgcgcacgc gatccgctgc gagggcagct tctgcgccac ctacgcgcag caggcgcgca
     2941 tcagcgcggg cgactcgatc ttcatcgacg acgtcgccat gcagtgccag ctcgaggcac
     3001 gcaaccacat ccgcgtaggc aagcgcctgc gcggccagat cgtcggcggc cattgccgcg
     3061 ccagcctgtc catccacgcg cgcacgatcg gcgcgaacag ccgcatccgc accgagctcg
     3121 aggtcggcat ggacaacggg ctggaacacg ccatccagga gaaggccgag gcgcgcgacg
     3181 cactcgagaa ccgcttgctc gagatcggca agatgctcac cttcgccgac cgccatcccg
     3241 atcgcgtgac acccgagatg ctgggacgtg ccgagcagac ggcgagcgcg ctgtcgggcg
     3301 agatcgagag cttgcgcagc gaggaggagg atctgcagca ccgcctcgcg ctcacccgcg
     3361 aggcacgggt gaatgcggag cgcgagatgt tcgagggctg catcgtgcgc atgggcgagc
     3421 agctgttcaa gctgtcgcag gaccgcgggc cgaccacggt gcgactggcc acccaggggc
     3481 tgggcgtgtt cccgctcgag gacgacagcc gcttcgacga gccgcagcgc ccggcggcgg
     3541 ccagccaggg ctcgtcccgg cgctgagcgt cgccaccgcg cccggcggcg acgcccgcgg
     3601 cacggcggct tacttcagcg cggcgagcgc ggcggcgtag tcgggctctt ccttgatctc
     3661 gggcacgagc tgcgaataca ccaccttgtc gttgccatcg acgaccacga ccgcgcgcgc
     3721 ggtgacgccg gcgagcgggc cggaggtgat cgcgacgccg tagtcctgcg cgaacttcgg
     3781 gttgcggaag gtcgacagcg tatgcacgtt cttcaggcct tcggtctcgc agaagcgctt
     3841 ggcggcgaag ggcaggtcgg cggacacgac cagcaccacg gtatccgcga ggccggcggc
     3901 ctcggcgttg aacttgcggg tcgaggtggc gcacaccggg gtgtccaggc tggggacgat
     3961 gttcagcacc ttgcgcttgc cggcgaaggc ctcgaggccc acgtcggcta gatcggcgcc
     4021 ggtgagcctg aaggcgggcg ccgcctcgcc ggcctgcggg aaacggcccg ccacgtcgat
     4081 cgggttgccg ccaagggtga ctttgctcat gtcgatctcc ttcaggtgag ggttgcgccg
     4141 gcacgtgcct gcgtcgccgg ctggtgggac cgctagccta caccaagccg atgcatgcag
     4201 gcgttctggt ccgcgcgcgg aggctccgca ccgaagtgcg ccgtggaagc gggccgggaa
     4261 gggctcgggc gaggcggggg tcccgacacc acgcagacgg agccggcctg atctatggtg
     4321 aagatcaggc cgcaacccga tggagcatcg tgatggaacc ggtggatctc ttctacagct
     4381 acgcgcacga ggacgaaaag ctgcgcgacg agctcgacgg ccacctcgcg ctgctgcggc
     4441 gcaagggcgt gatccgtccc tggcacgatc gcggcatcgt ccccgggcag gagtgggaca
     4501 agacgatcga cgagcagctc gccatggcag acctgatcct gctgctggtg agcatggact
     4561 tcctcaactc cgactatatc tggggcaagg agctcgccac cgcgatcgaa cgcgcccggc
     4621 gcggcgacgc cagcgtgatc ccggtgctgc tgcgcgcggt cgatctcgag gacgcgccct
     4681 tcgcccacct gcagggcttg cccaccgacc tgcgcccggt gacctcgtgg cccaaccgcg
     4741 acgaggcctg gaccgacgtc gccaagggca tccggcgcgc ggtcgaggcc atccgccagc
     4801 gctgggcgag cgcaccgccc ccggcacccc cgccggcagc cccgccggag acgccgtccg
     4861 taccgcgcgc gagcggtttc ggtacggcgg tcgcaagcgt gccgcttccg gaagagcctg
     4921 tccgcacgct cgaaatggcg cgtcccgcgc ccaggatggc cgcgacccgg gacgcccgca
     4981 ccgacgccct gctcgagcgc accatcgccg cgttcgcaaa cgagctcgcc ctcgcggcgc
     5041 gcatcaaggg cgtgcagggc atcggcagcg gcgaggcgga gggcctggcc gaggcgctga
     5101 tcgacatgcc cgcgcagaag cgcgtgctct gggtggacga ccgcccggaa aacaaccgcc
     5161 atgagctggc cgcgctggcg aaactgcagg tcgaggtggt gaacgtgcgc agcaccgggg
     5221 aagcgatcgc ctgcctcgat gcggacgagg aaggtttcga cctgatactg tcggattggg
     5281 accgagccga accggccgcc accgcatcga gcgccggact cgagctgcta cgcatgctcg
     5341 ccgcacgccc gcggcgaccg ccggtcatct tctatcacgc cgagttcgat ccctcgcgtc
     5401 gcgcggcgcg gcgcgagtgc ctgctgcgcg ccggcgcctt cggcgaggcg gtgcggccgg
     5461 gcgagctgct cgccctgatt cgcagcgcct tcgcgtcgga ctgagcgtcg ggcaggcctc
     5521 agcgcatggt ctgcaggcgc gcgatgcgct cctgggtgga ggggtgcgtc gagaacagcc
     5581 cgcgcaggcc gccgccggag agcgggttca tgatcatcat ctgcgcggtc tccgggtggc
     5641 gctcggcggt gtgcatcggg atgccgcgcg cgtaggcgtc gatcttggcg agcgcggcgg
     5701 cgagcgcggc gggatcgccg gagatctccg cgccaccgcg gtcggcgccg aactcgcggg
     5761 tgcggctgat cgccatctgg atcagcatgc cggcgagcgg ggcgaggatc atcatcgcga
     5821 tcgagacgat ggggttggga cgctcctcgc cctcgcggcc gccgaagaac atgccgaagt
     5881 tggccagcat cgagatcgcg cccgcgatgg tggccgagat ggtcg
//
LOCUS       DAOQNN010000186         5888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7849_length_5888_cov_7.150009, whole genome shotgun sequence.
ACCESSION   DAOQNN010000186 DAOQNN010000000
VERSION     DAOQNN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7849_length_5888_cov_7.150009"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PKV97_10450"
     CDS             <1..869
                     /locus_tag="PKV97_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06338.1"
                     /translation="TTGDTSAAVAATSSASAADTSGAAPVAASVLAAGAASTAAAQPG
                     TPTDPLAWLPAKHRVFGDDGKTLNLEASARKVAEAYGHAERRIGSGELPPKTAEEYKV
                     NVPAALGDKFKAEDLGKDPMLRAFFKDAHAAGMTQKQIDMTIGAWLERAPALVQGVKQ
                     LDKRAATAELRKTWATDAEFTEGVQRAFRAGEAFAGADFEGILGDYGNDPRIVRMLAA
                     VGKELAEDASAPAGAQAGFQADLEALTKSKAYMDPNNPEHLATKQKVAALFARTYGNE
                     PKRPGPIVITTG"
     gene            1065..1958
                     /locus_tag="PKV97_10455"
     CDS             1065..1958
                     /locus_tag="PKV97_10455"
                     /inference="COORDINATES: protein motif:HMM:NF040375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HNB06339.1"
                     /translation="MSFQVTEAMVQQYGTNFRTLFQQRQARLRPWCQVEAGIVGQSKS
                     VERIGKAEAYDITSRHADTKYVEVPHSRRWIDLQDKGWAELIDELDKIRMLADPTSPY
                     ASLATYALNRQIDDIIMSASRGNARTNSGVSALPAAQKIAAGGTSLTLAKLLTAKEIL
                     DSNEVDDDASMAADGQSTSEQAVRVVVVNAKMLTNLYGTTEIKSIDYNSVKALAQGQI
                     DTFLGFKFVRSERVAKDATATTGYAVCWSRSCMGLGIGKDIMTSIDPLPGKNYSVQVY
                     ARMSIGATRLEDEGVVEIACA"
     gene            1971..2675
                     /locus_tag="PKV97_10460"
     CDS             1971..2675
                     /locus_tag="PKV97_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06340.1"
                     /translation="MANFVADFQSIIDGPAYGQPLVNRTRASKGGGRLRAFEAIFRAP
                     ASGTAPAVGDKIIWGKLPVGARILGHLSRLDFGAGTAACTINLGDQFLAARHLAATAI
                     NAAGSAVPSAAVFSATCVADVTIGSAMLANVKGIGACGIGDVLSGTGIAAGAYVVAVD
                     KQARTVTMSAAATATNAAVTVTAQGTPYETSDDTSNVANGYASATDDATLVSVVAGAQ
                     IANNQVIALKVVYVQD"
     gene            2761..3351
                     /locus_tag="PKV97_10465"
     CDS             2761..3351
                     /locus_tag="PKV97_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06341.1"
                     /translation="MAATDVAICSNALLMLGAQPINDLTENNDRARLASNLYPAVRDA
                     LLRSFPWNCCIQRVVLAPDATAPAFDWTYQFQLPGDFVKALAVGELGAEHEFRIEGRK
                     LLSNDNPCLLRYVFRNTNPATWDDGLVNVLTLQMAAAMAYGITQSAALQDALQKTAMN
                     AMRAAKAVDGQDDTPETFGDLRLLRSRFARSDILSQ"
     gene            3353..5815
                     /locus_tag="PKV97_10470"
     CDS             3353..5815
                     /locus_tag="PKV97_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06342.1"
                     /translation="MPRLSLGSTNFTAGEISPRLYGRVDIDRYANAAKRLVNCYPVIQ
                     GGAKRRSGTRFVAAAKLGAAKRARLIPFVVSRDTAYMLEFGDSYLRVWPAGGGAMVLE
                     LATPYTAAMLDDIDYAQGADTMFVAHPAVPIQRLRRFSAAVFDLSAAPFTTTPFDEQG
                     HSPAATLTLSAATVGVGRTATASSGVFLPSDVGRQLFSGAGLAVVTGYTSATVLTVDV
                     SIAFAGVALASGAWTLDVSPQGLAKPAAKDPVASSIDLAGALSRAANLTLSAKTGAVT
                     VTASAGVFVAGDVGKTLYADNGVAAITAFTSATQVSATTSTDFAATFYAAGGWGITDA
                     VWRAEDVGKFVRINGGLCKITAFSSASLVKATILTALAGTVAAPPLAWSLESSVWSAA
                     AGYPRTVSMHEQRLVAAGSTKYPQTIWGSRTGEYLDFTKGVADTDSYSFTIANDEVNP
                     ISYVASLRNLIVHTYGGEFSLQGGIEKPITPTNVRIRPESGHGSKGVRPVTIGKESIF
                     VQRAGRKVRAMGYRYDFDGYASPDLSVLAEHITAGAVVQIAYQQEPDMLLLAVRGDGA
                     LLSCTIDRDQSVIGWAAHYTDGAFESVATLPNGDHEEVWAVVRRTVGGVEVRYIERFE
                     DAFAPQLAAAPGGYPPLAQPVVYGTTVDCGLSFDSVPGTATFTGLAHLEGKSVAIVAD
                     GSAMPQQVVTGGAVTLPRAAHRVLIGLPFTSMVQLLTPEAGGQTGTAQGNSMRTSEIT
                     LRFLDTIGAKVLDGDGTEQTVPFRRFGPDILDQPPEPFTGNVRIETLGWERGRAELTI
                     VQDQPLPMHLLSAVRKFQVNDG"
BASE COUNT          965 a         2121 c         1998 g          804 t
ORIGIN      
        1 ccaccaccgg cgacacgagc gcggccgtag ccgcgacatc cagcgccagc gcagccgaca
       61 cgtcgggggc agcgcccgtg gcggcgtccg tgctcgccgc cggcgcagcg tcgactgccg
      121 ctgcgcagcc gggcacgccg accgatccgc tggcgtggct gcccgccaag catcgcgtct
      181 tcggcgatga cggcaagacc ctcaacctcg aagcgtccgc gcgcaaggtc gccgaagcct
      241 acggccacgc tgaacggcgc atcggctcgg gcgagctgcc gcccaagacc gccgaggagt
      301 acaaggtcaa cgtgccggcc gcgctcggtg acaagttcaa ggccgaagac ctaggcaagg
      361 acccgatgct gcgggccttc ttcaaggacg cgcacgcggc cggcatgacc cagaagcaga
      421 tcgacatgac gatcggcgcc tggctcgagc gcgcgccggc gctggtgcaa ggcgtcaagc
      481 agctcgacaa gcgcgcggcc accgccgagc tgcgcaagac gtgggcgacc gatgccgagt
      541 tcaccgaagg cgtgcagcgc gccttccgcg ctggcgaggc gttcgccggc gccgacttcg
      601 aaggcatcct cggcgactac ggcaacgacc cgcgcatcgt gcgcatgctc gctgcggtcg
      661 gcaaggagct cgccgaagac gcctcggccc cggccggcgc gcaggccggc ttccaggccg
      721 atctggaggc gctcaccaag tccaaggcgt acatggaccc gaacaacccg gagcacctgg
      781 ccaccaagca gaaggtcgcg gcgctgttcg cacgcaccta cggcaacgag cccaagcgcc
      841 cgggcccgat cgtcatcact accggctgac gcgcgctgac gcgggcttga acaagcccgc
      901 gcggtcgacg tacattgccc ccacacggcc cgcgtgaggc agcgcggaga cccgtagcag
      961 atgccccacc gcaccgctgc agccggcgga tcgtggcgta gcaaccgggc cccgcgaggg
     1021 acaccccgag gcgaacttca ccgttcaact ccgggagtat ccccatgagc tttcaagtca
     1081 cagaagcgat ggtgcagcag tacggcacca actttcgcac gctgttccag caacgccaag
     1141 cgcgcctgcg cccctggtgc caggtcgaag ccggcatcgt cggccagtcc aagagcgtcg
     1201 agcgcatcgg caaggccgag gcgtatgaca tcaccagtcg gcacgccgac accaagtacg
     1261 tcgaggtgcc gcactcgcgg cgctggatcg acctgcagga caagggctgg gccgagctga
     1321 tcgacgagct cgacaagatc cgcatgctcg ccgatccgac ctcgccctac gcgtcgctcg
     1381 ccacctacgc gctcaaccgc cagatcgacg acatcatcat gtcggcgtcg cgcggcaacg
     1441 cgcgcacgaa ctccggggtg tcggccttgc cggcagcgca gaagatcgcc gcgggcggca
     1501 cctcgctcac gctggccaag ctgctgacgg ccaaggagat cctcgactcc aacgaggtcg
     1561 acgacgacgc gagcatggcg gccgacggcc agtccacgag cgagcaggcc gtgcgcgtcg
     1621 tggtggtcaa cgccaagatg ctcacgaacc tgtacggcac gaccgagatc aagtcgatcg
     1681 actacaactc ggtgaaggcg ctcgcgcagg gccagatcga caccttcctc ggcttcaagt
     1741 tcgtgcgcag cgagcgcgtg gccaaggacg ccaccgccac caccggctac gccgtgtgct
     1801 ggtcgcgctc gtgcatgggc ctggggatcg gcaaggacat catgacctcg atcgacccgc
     1861 tgcccggcaa gaactactcg gtccaggtct acgcccgcat gtcgatcggc gccacgcggc
     1921 tcgaagacga gggcgtggtc gagatcgcct gcgcgtaagg agaaacccac atggcaaact
     1981 tcgttgcgga cttccagtcc atcatcgacg gcccggccta cggccagccg ctcgtcaacc
     2041 gcacgcgcgc gtccaaggga ggcggccgcc tgcgcgcatt cgaggcgatc ttccgcgcac
     2101 cggcctccgg cacggcgccg gccgtcggcg acaagatcat ctggggcaag ctgccggtag
     2161 gcgcgcgcat cctcgggcac ctgtcgcggc tggacttcgg cgccggcacc gcggcgtgca
     2221 cgatcaacct cggggaccag ttcctcgcgg cgcgtcacct ggccgccacc gccatcaacg
     2281 ccgccggcag cgcggtgccg tccgccgcag tgttcagcgc gacctgcgtg gccgacgtga
     2341 ccatcggcag cgcgatgctc gccaacgtca agggcatcgg cgcgtgcggc atcggcgacg
     2401 tgctgagcgg caccggcatc gccgccggcg cctacgtggt cgccgtcgac aagcaagcgc
     2461 gcacggtgac gatgagcgcg gcggcgacgg ccaccaatgc ggccgtcacc gtgacggcgc
     2521 agggtacgcc ctacgagacg agtgacgaca cctccaacgt ggccaacgga tacgcctcgg
     2581 ccaccgacga cgcgacgctc gtgagcgtgg tggccggcgc gcagatcgcc aacaaccagg
     2641 tgatcgcgct caaggtggtg tacgtgcagg actgatgtct tgacgccccc acccctgggg
     2701 gctttctcct tgaccggggg gcctcttgcg ccccccgctc tttcttgaag gatcggcaag
     2761 atggcggcca ccgacgtagc gatctgctcc aacgcgctgc tgatgctcgg cgcgcagccg
     2821 atcaacgacc tcaccgagaa caacgatcgt gcgcgcctgg cgtccaacct gtacccggcc
     2881 gtgcgcgacg cgctgctgcg cagtttcccg tggaactgct gcattcagcg cgtggtgctg
     2941 gcgccggacg cgacggcgcc tgcgttcgac tggacgtacc agttccagct tcccggcgac
     3001 ttcgtcaagg cgctcgccgt gggcgagctc ggcgccgagc atgagttccg catcgaaggc
     3061 cgcaagctcc tgagcaacga caacccctgc ctgctgcgct acgtgttccg caacacgaac
     3121 ccggcgacat gggatgacgg cctggtcaac gtcctcacgc tgcagatggc cgcggcgatg
     3181 gcctacggca tcactcagtc cgcggcgctg caggacgcgc ttcagaagac cgccatgaac
     3241 gccatgcgcg cggccaaggc ggtcgacggc caggacgaca cgcccgagac cttcggcgac
     3301 ctgcggctgc tgcgctcgcg ctttgcccgc agcgacatcc tgagccagtg acatgccgcg
     3361 cctgagcctg gggtcgacga acttcactgc cggcgagatc agcccgcggc tgtacggccg
     3421 cgtcgacatc gaccgctacg ccaacgccgc caagcgcctc gtcaactgct accccgtgat
     3481 ccagggcggc gccaagcgca ggagcggcac gcgcttcgtc gcggccgcca agctgggcgc
     3541 ggccaagcgc gcgcgcctga tcccgttcgt cgtgagccgc gacacggcgt acatgctgga
     3601 gttcggcgac tcgtacctgc gcgtgtggcc cgccggcggc ggcgcgatgg tgctcgagct
     3661 cgccacgccc tacacggccg cgatgctcga cgacatcgac tacgcgcagg gcgccgacac
     3721 gatgttcgtc gcgcaccccg ccgtgccgat ccagcggctg cggcgcttct cggctgccgt
     3781 cttcgacctg tcggcggcgc ccttcacgac cacgccgttc gacgagcagg ggcactcgcc
     3841 ggccgccacc ttgaccctct cggccgcgac ggtgggcgtg gggcgcaccg cgacggcgag
     3901 tagcggcgtc ttcctgccca gcgacgtggg ccgccagctt ttcagcggcg cgggcctggc
     3961 cgtcgtcacc ggctacacgt cggcgacggt gctcacggtg gacgtgtcga tcgcatttgc
     4021 cggcgtcgcg ctggcgtccg gcgcgtggac gctggacgtg tcgccgcagg gcctggccaa
     4081 gccggcggcc aaggacccgg tggcgtcgtc gatcgacctg gccggcgccc tgtcgcgcgc
     4141 ggccaacctc acgctgtcgg ccaagaccgg cgccgtcacc gtcaccgcgt cggcgggcgt
     4201 gttcgtcgcg ggcgacgtgg gcaagacgct gtacgccgac aacggcgtgg ccgcgatcac
     4261 cgccttcacc agcgcgacgc aggtgagcgc caccacgagc accgacttcg ctgccacctt
     4321 ctacgccgca ggcggctggg gcatcaccga tgcggtatgg cgcgccgagg atgtgggcaa
     4381 gttcgtgcgc atcaacggcg gcttgtgcaa gatcacggcg ttctcgtcgg cgagcctggt
     4441 gaaggccacc atcctgaccg cgctggccgg caccgtcgct gcgccgccgc tggcgtggtc
     4501 gctggagtcc tcggtctggt cggccgcggc gggctacccg cgcacggtgt cgatgcacga
     4561 gcagcgactg gtggccgcgg ggtcgacgaa gtacccgcag acgatctggg gcagccgcac
     4621 cggcgagtat ctggacttca ccaagggagt ggccgacacc gactcgtaca gcttcaccat
     4681 cgccaacgac gaggtcaacc cgatcagcta cgtggcgagc ctgcgcaatc tcatcgtgca
     4741 cacctacggc ggcgagttct cgctgcaagg cggcatcgaa aagcccatca cgccgaccaa
     4801 cgtgcgcatc cgccccgagt ccggccacgg atcgaagggc gtgcgcccgg tgacgatcgg
     4861 caaggagtcg atcttcgtgc agcgcgccgg ccgcaaggtg cgcgcgatgg gctaccggta
     4921 cgacttcgac ggctacgcga gcccggatct gtcggtgctg gccgagcaca tcacggcggg
     4981 cgctgtcgtg cagatcgcgt accagcagga gcccgacatg ctgctgctgg ccgtgcgtgg
     5041 cgacggcgcg ctactgtcgt gcaccatcga ccgcgaccag tcggtgatcg gctgggcggc
     5101 gcactacacc gacggagcct tcgagtcggt ggccacgctg cccaacggcg accatgaaga
     5161 ggtgtgggcc gtcgtgcggc gcaccgtggg cggtgtcgag gtgcgctaca tcgagcgctt
     5221 cgaagacgcc ttcgcgccgc agctcgccgc cgcaccgggc gggtatccgc cgctcgcgca
     5281 gcctgtggtg tacggcacga cggtcgactg cggcctgagc ttcgacagcg tgccgggaac
     5341 ggcgaccttc acgggcctcg cgcacctcga ggggaagtcg gtggcgatcg tcgccgacgg
     5401 ctcggcgatg ccgcagcagg tggtgacggg tggcgccgtc acgctgccgc gcgctgcgca
     5461 ccgcgtgctg atcgggctgc ccttcacgag catggtgcag ctgctgacac ccgaggccgg
     5521 cggccagacc gggaccgcgc agggcaacag catgcgcacg agcgagatca cgctgcggtt
     5581 cctcgacacc atcggcgcca aggtgctcga cggcgacggc accgagcaga cggtgccgtt
     5641 ccgacgcttc ggccccgaca tcctcgacca gccgcccgag ccgttcaccg gcaatgtgcg
     5701 catcgagacg ctcggctggg agcgcggccg cgccgagctg acgatcgtgc aggaccagcc
     5761 gttgccgatg cacctgctgt cggccgtgcg caagttccag gtcaacgatg gctgaagcaa
     5821 tggcgctgtg gatcgaagcc ccggcaggcg ccggcgcggt cgacgagctc gccgcggcgc
     5881 tgcacccg
//
LOCUS       DAOQNN010000187         5885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7854_length_5885_cov_9.963636, whole genome shotgun sequence.
ACCESSION   DAOQNN010000187 DAOQNN010000000
VERSION     DAOQNN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7854_length_5885_cov_9.963636"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /gene="glmS"
                     /locus_tag="PKV97_10475"
     CDS             complement(<1..1520)
                     /gene="glmS"
                     /locus_tag="PKV97_10475"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNB06343.1"
                     /translation="MCGIVTAIATNNVVPVLLEGLRKLEYRGYDSAGLAVLAGELTRL
                     RSCGRVAELAALADARQLSAHIGIAHTRWATHGVPSERNAHPHISGGLAVVHNGIIEN
                     FESIKADLQGKGYVFESETDTEAIAHLVHYKLQANTDLFEAVRQATNELVGAYAIGVV
                     AEADPFRVIVARRGSPLLLGIGEDGMYAASDTAALLQVTRKIVYLEDGDLAELRRDSY
                     IVIRSDGSPVEREVHISSLSADAVELGKFRHYMQKEIFEQPQALAQTLELIAGGAGIT
                     PQHFGARAGEVFDGVRRVLVLACGTSYHAGLVARYWIESIAKLPVTVEIASEYRYRES
                     VPDPDTLVVVISQSGETADTLAALKHAKALGMQRTLAICNVPESAIVRETALRFITRA
                     GPEIGVASTKAFTTQLAAFALLTLALAKINGRLSAEDEERRLAALRHLPVAVQKVLEL
                     EPEIEQWAQRFAGKEHALFLGRGSHWPIAMEGALKLKEISYIHAEAYAAGELKHGPL"
     gene            complement(1592..2953)
                     /gene="glmU"
                     /locus_tag="PKV97_10480"
     CDS             complement(1592..2953)
                     /gene="glmU"
                     /locus_tag="PKV97_10480"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNB06344.1"
                     /translation="MQVVVLAAGQGKRMRSVLPKVLQPIAGKPMLAHVLDAARTLDAQ
                     RVCVVYGHGGEVVRERLDAVDLAWARQETQLGTGHAVQQALPHLDDEHVALVLYGDVP
                     LIGVPTLRRLVAAAGDARLALLTVEMDNPTGYGRILRDAAGKVVRIVEEKDASDEERK
                     VREVNTGILVAPVRHLRAWLGRIGNDNAQGEYYLTDIIGLAVADGVEVTTVQPDAFAE
                     TLGVNNKMQLAELERIHQGNIARRLMEEGVTLIDPARIDVRGTLTVGRDVEIDVNCVF
                     EGEVELGDGVRIGAHCVVRDARIGAGTRLEPFSHVDSTTMGQACVIGPYARTRPGTVL
                     GTDVHLGNFVEIKNSVIADHSKANHLAYVGDADVGSKVNIGAGTITCNYDGANKHRTI
                     IEDEVFIGSDTQLVAPVRVGRGATLGAGTTLTKDAPAGQLTVSRSKQISLEHWKRPVK
                     QPR"
     gene            3230..4636
                     /locus_tag="PKV97_10485"
     CDS             3230..4636
                     /locus_tag="PKV97_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNB06345.1"
                     /translation="MKAPAAQQMIQNRTFDEIQVGDFAQLVRTLRPEDIHLFAAMSGD
                     VNPTHVDLEYARSSQFREVVGHSMWGSTLISTVLGTEFPGPGTVYVSQGLNFWRPITI
                     GDTLTITVTCKEKFEHNHHIIFDCLAVNQDGLKVIDGIAEVMAPTEKISRARVVLPEV
                     TVSDRELRYRHLLSVSAGLSPIPIAVAHPCDRESLLGPVQAARAGLVEPILVGPESRI
                     RAVAEEHEIDIKGLRIVDTEHSHASAEVAVALCRDGGAEALMKGSLHTDEMMSQVIKH
                     LRTGRRISHVFLADVPTYPHPLMITDAAIMIEPTLEDKVDIIQNAIELAHILGLPEPK
                     VAILSAVETVTSKIRSTIDAAALCKMADRGQIKGGLLDGPLAFDNAVSLVAAKTKGIK
                     SVVAGNADILVVPDLESGNMVAKQLEYLANALMAGVVLGARVPIVLTSRADTAETRTA
                     SCAVVQLMAHKKREALNA"
     gene            4633..5817
                     /locus_tag="PKV97_10490"
     CDS             4633..5817
                     /locus_tag="PKV97_10490"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNB06346.1"
                     /translation="MKAVLVLNAGSSSLKFALYPVDGDVAAAPAVSGQVEGIGARPEV
                     TFKTAAGERIQEPVVTGGSQGEQHRDALEHVFALLVRHNPALDIVAAGHRIVHGGEHY
                     SAPTRLDEAVLRTLDTFVPLAPLHQPHNLRAVRAVAGLMPQVPQVGCFDTAFHRTQPA
                     VAQAFALPRRLSAEGIKRYGFHGLSYDYVARQLPAVVGERARGAVVIAHLGNGASMCA
                     LRDGRSVASTMGFTAVEGLMMGTRTGSLDPGVMLYLMEQKGMDAKALTSLLYKESGLL
                     GVSGISQDMRTLLASDAPEAKEAVDLFCYRIARELGSLAAAAGGLDALVFTGGIGEHA
                     APVREKVAALAAWMGIEVDPAANADHARRIDTAASRVAVAVVPTNEERMIARYTAETL
                     GL"
BASE COUNT          878 a         1985 c         2125 g          897 t
ORIGIN      
        1 agcgggccgt gcttgagctc gccggcggcg taggcctcgg cgtggatgta ggagatctcc
       61 ttgagcttga gcgcgccttc catggcgatc ggccagtggc tgccgcggcc caggaagagc
      121 gcgtgctcct tgccggcgaa gcgctgcgcc cactgctcga tctcgggctc gagctcgagc
      181 accttctgca ccgcgaccgg caggtggcgc agcgcggcga ggcggcgctc ctcgtcttcg
      241 gcggacaggc ggccgttgat cttggcgagc gcgagcgtga gcagcgcgaa ggcggcgagc
      301 tgggtggtga aggccttggt ggaggcgacg ccgatctcgg ggccggcgcg ggtgatgaag
      361 cgcagcgcgg tctcgcgcac gatggcggat tcgggcacgt tgcagatcgc cagcgtgcgc
      421 tgcatgccga gcgccttggc gtgcttgagc gcggccaggg tgtcggcggt ctcgcccgac
      481 tgcgagatga ccacgaccag ggtgtcgggg tcggggacgg actcgcggta gcggtattcg
      541 ctggcgatct cgacggtgac cggcagcttg gcgatggatt cgatccagta gcgcgcgacc
      601 aggccggcgt ggtagctggt gccgcaggcg agcacgagca cgcggcgcac accgtcgaag
      661 acttcgcccg cgcgcgcgcc gaagtgctgc ggggtgatgc cggcaccgcc ggcgatcagc
      721 tccagcgtct gcgccagggc ctgcggctgc tcgaagattt ccttctgcat gtagtggcgg
      781 aacttgccga gttcgaccgc gtcggccgac aggctggaga tgtgcacctc gcgctcgacc
      841 gggctgccgt cggagcggat gacgatgtag ctgtcgcggc gcagctcggc gaggtcgccg
      901 tcttccagat agacgatctt gcgcgtgacc tgcagcagcg cggcggtgtc ggaggcggcg
      961 tacatgccgt cctcgccgat gccgagcagc agcggcgagc cgcggcgcgc gacgatgacg
     1021 cggaaggggt cggcctcggc gaccacgccg atggcgtagg cgccgacgag ctcgttggtg
     1081 gcctggcgca cggcctcgaa gaggtcggtg ttggcctgca gcttgtagtg gaccaggtgg
     1141 gcgatggcct cggtgtcggt ctcggactcg aagacgtagc ccttgccctg caggtcggcc
     1201 ttgatggatt cgaagttctc gatgatgccg ttatgcacca cggcgaggcc gcccgagatg
     1261 tgcgggtggg cgttgcgctc ggagggcacg ccgtgggtgg cccagcgcgt gtgggcgatg
     1321 ccgatgtggg cggagagctg gcgcgcatcg gcgagcgcag cgagctcggc cacgcggccg
     1381 caggagcgca ggcgggtgag ctcgccggcg agcacggcga ggccggccga gtcgtagccg
     1441 cggtactcga gcttgcgcag gccctcgagc aggaccggga cgacgttgtt ggttgcgatt
     1501 gcggtgacga tgccacacat ggcggggctt ccttgtcggt ctttggattc ggtgcggagg
     1561 gcggggcggg cgaatcggct ggcggagggg ctcagcgcgg ctgcttgacc gggcgcttcc
     1621 agtgttcgag gctgatctgc ttgctgcgcg acacggtgag ctgtccggcg ggggcgtcct
     1681 tggtgagcgt ggtgccggcg cccagggtgg cgccgcggcc cacgcgcacc ggcgcgacga
     1741 gctgggtgtc ggagccgatg aagacctcgt cctcgatgat ggtgcggtgc ttgttggcgc
     1801 cgtcgtagtt gcaggtgatg gtgccggcgc cgatgttgac cttgctgccg acgtcggcgt
     1861 cgcccacgta ggcgaggtgg ttggccttgg agtggtcggc gatgacgctg ttcttgatct
     1921 cgacgaagtt gcccaggtgc acgtcggtgc cgagcacggt gccggggcgg gtgcgggcgt
     1981 agggcccgat cacgcaggcc tggcccatgg tggtgctgtc gacgtggctg aagggctcga
     2041 gccgggtgcc ggcgccgatg cgggcgtcgc ggacgacgca gtgggcgccg atgcgcacgc
     2101 cgtcgccgag ctcgacctcg ccctcgaaga cgcaattgac gtcgatctcg acgtcgcgcc
     2161 cgaccgtgag cgtgccgcgc acgtcgatgc gcgccgggtc gatcagggtg acgccctctt
     2221 ccatcaggcg gcgggcgatg ttgccctggt ggatgcgctc gagctcggcg agctgcatct
     2281 tgttgttgac gcccagcgtc tcggcgaagg cgtcgggctg cacggtcgtc acctcgacgc
     2341 cgtcggccac ggccaacccg atgatgtcgg tgaggtagta ctcgccctgg gcgttgtcgt
     2401 tgccgatgcg gccgagccag gcgcgcaggt ggcggaccgg ggcgaccagg atgccggtgt
     2461 tcacctcgcg caccttgcgc tcctcgtcgg aggcgtcctt ctcctcgacg atgcgcacca
     2521 ccttgccggc ggcgtcgcgc aggatgcggc cgtagccggt cgggttgtcc atctcgacgg
     2581 tgagcagcgc caggcgcgcg tcgccggcgg cggcgaccag gcggcgcagc gtgggcacgc
     2641 cgatcagcgg cacgtcgccg tacagcacca gggccacgtg ctcgtcgtcg aggtggggca
     2701 gcgcctgctg caccgcatga ccggtaccga gctgcgtctc ctgccgcgcc caagccaggt
     2761 cgacggcatc gaggcgctcg cgcaccactt cacccccatg gccgtacacc acgcagacgc
     2821 gctgggcgtc gagcgtgcgg gcggcatcga gcacgtgggc cagcatcggc ttgccggcga
     2881 tgggttggag gaccttgggc agcaccgagc gcatgcgctt gccctggccg gcagcgagaa
     2941 cgacgacttg catggttgat tccgtggcga aatgaggaga ttcgacaggt ttcgggcgca
     3001 caaccgaccc catccgcgcc cgcagatcgt aaaaacgcgc ggagtctagc acgcgacccg
     3061 cgcagccaat cgtgagcaat cgcatggtgc gggcatgaaa aacatccgcg ccgcaaggaa
     3121 acgtgcgtct gattgacgca ttgcacaatt cgatcgtttt agcgcatgtg gcagtcacat
     3181 acaaggtcta gaatcgcgtg ttgcaatgca tccaagaacg agagttgcca tgaaagctcc
     3241 tgccgcacaa cagatgatcc agaaccgcac cttcgacgag atccaggtgg gcgacttcgc
     3301 ccagctcgtc cgcacgctgc gccccgagga catccacctg ttcgccgcca tgtcgggcga
     3361 cgtcaacccg acccacgtcg atctcgaata cgcacgctcc agccagttcc gcgaggtcgt
     3421 cggccacagc atgtggggca gcacgctgat ctccaccgtg ctcggcaccg agttccccgg
     3481 ccccggcacg gtgtacgtgt cgcaggggct gaacttctgg cgcccgatca cgatcggcga
     3541 cacgctgacg atcacggtca cctgcaagga aaagttcgag cacaaccacc acatcatctt
     3601 cgactgcctg gcggtgaacc aggacggtct caaggtgatc gacggcatcg ccgaggtgat
     3661 ggcgccgacc gagaagatct cgcgcgcgcg cgtcgtgctg cccgaggtca ccgtgtccga
     3721 ccgcgagctg cgctaccgcc acctgctgtc ggtgtcggcc ggcctgtcgc cgatcccgat
     3781 cgcggtggcc cacccctgcg accgcgaatc cctgctcggc ccggtccagg cggcacgcgc
     3841 cggcctggtc gagccgatcc tggtcggccc cgagtcgcgc atccgcgccg tggccgaaga
     3901 acacgagatc gacatcaagg gcctgcgcat cgtcgacacc gaacacagcc acgcctcggc
     3961 cgaggtggcc gtcgccctgt gccgcgacgg cggcgccgag gcgctgatga agggctcgct
     4021 gcacaccgac gagatgatga gccaggtgat caagcacctg cgcaccggcc gccgcatcag
     4081 ccacgtcttc ctcgccgacg tgccgaccta cccgcacccg ctgatgatca ccgacgcggc
     4141 gatcatgatc gagcccaccc tcgaggataa ggtcgacatc atccagaacg cgatcgagct
     4201 cgcccacatc ctcggcctgc ccgaacccaa ggtggcgatc ctgtcggcgg tcgagaccgt
     4261 gacctcgaag atccgctcga cgatcgacgc cgccgcgctg tgcaagatgg ccgaccgcgg
     4321 ccagatcaag ggtggcctgc tcgacggccc gctcgccttc gacaacgcgg tgtcgctggt
     4381 cgccgccaag accaagggca tcaagtcggt cgtggccggc aacgccgaca tcctggtggt
     4441 gcccgacctc gagtccggca acatggtcgc caagcagctc gaatatctcg ccaacgcgct
     4501 gatggccggc gtggtgctgg gcgcgcgcgt gccgatcgtg ctgaccagcc gtgccgacac
     4561 cgccgagacg cgcaccgcgt cgtgcgcggt cgtgcagctg atggcccaca agaagcgcga
     4621 ggccctgaac gcatgaaggc cgtcctcgtt ctcaatgcgg gctcgtcgag cctgaagttc
     4681 gcgctctacc cggtcgacgg tgacgtggcc gccgcgccgg cggtgtccgg ccaggtcgaa
     4741 ggcatcggcg ccagacccga ggtcaccttc aagaccgccg cgggcgaacg catccaggag
     4801 ccggtggtga ccggcggcag ccagggcgag cagcatcgcg acgcgctcga gcacgtcttc
     4861 gcgctgctcg tccgccacaa cccggcgctc gacatcgtcg ccgccggcca ccgcatcgtg
     4921 cacggcggcg agcactacag cgcgccgacc cggctcgacg aggcggtgct gcgcacgctc
     4981 gacaccttcg tgccgctcgc cccgctgcac cagccgcaca acctgcgcgc ggtgcgcgcg
     5041 gtcgccgggc tgatgcccca ggtgccgcag gtgggctgct tcgacaccgc cttccaccgc
     5101 acccagccgg cggtcgcgca ggccttcgcg ctgccgcgca ggctgtcggc cgagggcatc
     5161 aagcgctacg gcttccacgg cctgtcctac gactacgtcg cgcgccagct gccggcagtg
     5221 gtcggcgagc gcgcccgcgg cgcggtggtg atcgcccacc tcggcaacgg cgcgtcgatg
     5281 tgcgcgctgc gcgacggtcg cagcgtcgcg tcgacgatgg gcttcaccgc ggtcgagggc
     5341 ctgatgatgg gcacccgcac cggcagcctc gaccccggcg tgatgctcta cctgatggag
     5401 cagaagggca tggacgccaa ggcgctcacc agcctgctct acaaggaatc gggcctgctc
     5461 ggcgtgtcgg gcatcagcca ggacatgcgc accctgctcg cctccgacgc ccccgaggcc
     5521 aaggaagcgg tggacctgtt ctgctaccgc atcgcgcgcg agctcggctc gctggctgcg
     5581 gcggcgggcg ggctggacgc gctggtgttc accggcggca tcggcgagca cgccgcgccg
     5641 gtgcgcgaga aggtggccgc gctggcggcg tggatgggca tcgaggtcga ccccgccgcc
     5701 aacgccgacc acgcccgccg catcgacacc gcggccagcc gcgtcgcggt cgccgtggtg
     5761 ccgaccaacg aagagcgcat gatcgcgcgc tacacggcgg agacgctggg gctgtaggcg
     5821 gcgcgggcgg cgcgcatcgg ccagcgccgg cgcgccgcgc ttcatccatc ggccggatcg
     5881 cgact
//
LOCUS       DAOQNN010000188         5874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7873_length_5874_cov_8.706135, whole genome shotgun sequence.
ACCESSION   DAOQNN010000188 DAOQNN010000000
VERSION     DAOQNN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7873_length_5874_cov_8.706135"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PKV97_10495"
     CDS             complement(<1..252)
                     /locus_tag="PKV97_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB06347.1"
                     /translation="MELAKSFEPADIERRWYPEWESRGYFDAGLDKSNPAAFCILLPP
                     PNVTGTLHMGHGFNQTIMDALTRYHRMRGWNTLWQPGTDH"
     gene            complement(457..1206)
                     /locus_tag="PKV97_10500"
     CDS             complement(457..1206)
                     /locus_tag="PKV97_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06348.1"
                     /translation="MRGWEDDSDDELERADDLLGQADALLRRHRGDATPASPRTPSPG
                     GAGPAEPADAAPAISPATMQAFDDDDLPILTEVVDDLDLPAEIFQPVEAPFAAAAVAS
                     TAAMPSFVRSLAAAQFTPAGEPAASTVRDAAPEPVTQPEPPAPQTPHPQSAPSAAESA
                     AASPPARSAAAQEQQLAERLVELDTAIAREIDEWAARELPQIIARELERLGERIQDEA
                     LAHLRATLLPGISARVSSLFDDRPHDVPPAR"
     gene            complement(1203..1643)
                     /locus_tag="PKV97_10505"
     CDS             complement(1203..1643)
                     /locus_tag="PKV97_10505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004390392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HNB06349.1"
                     /translation="MAPRVQFYHNTPDRLALAHELAAGAHARGRKVALRCTDATQLRR
                     LDQALWTAVPLDFVPHVGVGSALAAETPVVLGAAGEPCPWPHADMLFNLADDVPAEAE
                     GFRMVVEIVGRDAAEVHAARLRWAAYRQRGYELKAFDAERRVAL"
     gene            complement(1663..3162)
                     /locus_tag="PKV97_10510"
     CDS             complement(1663..3162)
                     /locus_tag="PKV97_10510"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNB06350.1"
                     /translation="MEFTIKTGSPAKLKTGLLVLGAFAEGRLPALSAAADGAAEGRLA
                     ALIKRGDLEDKAGATLLVHDLPGVHAERVLLVSLGKHDEFGDKAYRDALAAAAKAISA
                     GPAKDAVVALADAELSGRDMAWRLQQAARILADGAYRFDALKSDKKTRKERGAKKLCL
                     LVSCELGAELDTAVLQGHAIASGMALAKDLGNLPGNHCTPTHLAETAESLGKQYKFDV
                     EVLERDDMEKLGMGSFLSVARGSHQPPKFIVMHYKGGKAKAKPVVLVGKGITFDTGGI
                     SLKPAAEMDEMKFDMCGAASVLGTFKAVAQMGLPINLVGLVPTTENMPGGGATKPGDV
                     VTSMSGQTIEVLNTDAEGRLILCDALTYAERFKPECVIDIATLTGACVVALGKIPSGL
                     LANDDELAAEILRRGTESGDRAWQLPLWDEYQELLKSNFADMGNIGGRYAGTITAACF
                     LSRFAKAYKWAHLDIAGTAWVSGDAKGATGRPVPLLSSFLIGRARAHAA"
     gene            3301..4383
                     /gene="lptF"
                     /locus_tag="PKV97_10515"
     CDS             3301..4383
                     /gene="lptF"
                     /locus_tag="PKV97_10515"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HNB06351.1"
                     /translation="MIFRRALQREFAQTAAAVFVALFAILISTVLIRLLGQAAGGRVP
                     PDAVVALIGFGALAQLPTVLTLTLFIAILVSLSRSYRDSEMVVWFASGVPLTAWIRPV
                     LMFALPVVLVAGGGSLYLTPWSQQKSAEYRERLENRDDTHRVAPGVFRESASAQRVFF
                     VEVGAGEDGRVRNVFVSEQTRSGQLVVIASAEGYLRGDADGRRFVVLEKGRRYDGTPG
                     TPEYRVMEFDRYEVQVEQRQVAGQQARIRALPTAVLLAQPEPRGLGELVGRFGVPAVA
                     LLLALMAIPLSFVNPRAGRANNLLVALLVYLLYSNAISVFQSWVAQGKLPFVAGLLLP
                     HVVVLAVLALMFHKRLAISPFWRRRA"
     gene            4463..5539
                     /gene="lptG"
                     /locus_tag="PKV97_10520"
     CDS             4463..5539
                     /gene="lptG"
                     /locus_tag="PKV97_10520"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNB06352.1"
                     /translation="MSPVLLRYLAREIFAASALVLVAFLGLFAFFDFINELEDVGKGG
                     YTLYQALVYVGLTLPARVYELMPIAVLIGTLYALATLAKNSEITVMRASGMSNALLTS
                     VLGLIGGVFAVLIFLVGEYVAPPAEAAAEEWKLTATQATVSQQLRSGLWVKDGRLVLN
                     VRTLMPDRSMRGARIYAFDEDYALESIAEAATGVFDGSGEWKLLDVQRTRFHGDRTEV
                     ERLPEIRWRSELTPEVLGVLMVSPEHMPFGRLWTYINHLRDNNQNADRFEIALWKKLI
                     YPFTALVMMALAMPFGFIHDRMGGASARIFMGVMLGVGFHLLNGLFSNLGMINAWPPF
                     MAALTPSLIFLAAAFWLMHRVERR"
     gene            complement(5764..>5874)
                     /locus_tag="PKV97_10525"
     CDS             complement(5764..>5874)
                     /locus_tag="PKV97_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNB06353.1"
                     /translation="KLYYLRQRSGKSARIKEKLDYKAAAAKRAAQQQQEA"
BASE COUNT          775 a         1959 c         2144 g          996 t
ORIGIN      
        1 gtggtcggtg cccggctgcc acagggtgtt ccagccccgc atgcggtggt agcgcgtgag
       61 cgcgtccatg atcgtctggt tgaagccgtg ccccatgtgc agcgtgccgg tgacgttggg
      121 cggcggcagc aggatgcaga aggcggcggg gttcgacttg tcgagcccgg cgtcgaaata
      181 gccgcgggat tcccattccg ggtaccagcg acgttcgatg tcggcgggct cgaagctctt
      241 ggccagttcc atggtgttct acgcgcgtga agacaaagcg cggattatag cggatggagc
      301 accggcgccc cggtacgagc gcgccatggg gcggtgtcgc gcccttctcc cctgccgaac
      361 cgcggtggtc cgccgtggga gcgacgcgag tcgcgattgc ggtggccgtc cggccgcagc
      421 gcaaggcagc ggtcccgttc gcgactcgcg tcgctcctac cgggcaggcg gaacgtcgtg
      481 gggacggtcg tcgaacaggc tgctgacgcg tgcagagatc ccgggcagca acgtggcgcg
      541 caggtgggcg agggcttcgt cctggatgcg ctcgccgagg cgctcgagtt cgcgggcgat
      601 gatctgcggc agctcgcgcg cggcccattc gtcgatttcg cgcgcgatcg cggtgtcgag
      661 ctcgaccagg cgttcggcga gctgctgctc ttgcgccgcg gcgctccgtg ccggtggcga
      721 tgccgccgcg gattcggcgg cggagggcgc ggactgcggg tggggtgtct gcggcgcggg
      781 cggttcgggc tgcgtgacgg gctccggcgc tgcatcgcgc acggtcgacg cggcaggctc
      841 ccccgcaggc gtgaattgcg ccgctgcaag gctgcgcacg aaggacggca tggccgccgt
      901 gctcgccacc gcggccgccg cgaacggcgc ctcgaccggc tggaagattt ccgcaggcag
      961 atcgaggtcg tcgacgacct cggtcaggat cggcaggtcg tcgtcgtcga aggcctgcat
     1021 ggtcgcgggc gagatcgccg gcgcggcatc ggcgggctcg gcggggcccg cgccgcccgg
     1081 cgatggtgtg cggggggagg ctggcgtggc gtccccgcga tggcggcgca gcagcgcgtc
     1141 ggcctggccg agcaggtcgt cggcgcgctc gagttcgtcg tcggagtcgt cttcccagcc
     1201 tctcacagcg ccaccctgcg ttcggcgtcg aaggccttca gttcgtaacc gcgctgccgg
     1261 taggcggccc agcgcaggcg ggcagcgtgc acttcggcgg cgtcgcggcc gacgatctcg
     1321 acgaccatgc gaaacccttc cgcctcggcg gggacgtcgt cggcgaggtt gaacagcatg
     1381 tcggcgtgcg gccacgggca gggctcgccg gcggcgccca gcaccaccgg ggtctccgcg
     1441 gccagcgccg acccgacgcc gacgtggggc acgaagtcga gcggcaccgc ggtccatagc
     1501 gcctggtcga gtcggcgcaa ctgggtcgcg tcggtgcaac gcagtgccac cttgcggccg
     1561 cgtgcgtgcg ccccggcggc cagctcgtgc gcgagcgcga ggcggtcggg ggtgttgtga
     1621 tagaactgca cgcgcggggc catggcggcc gccggccggc gctcaggccg catgggcgcg
     1681 ggcgcgcccg atcaggaagc tgctcagcag cggcaccggg cggccggtcg cgcccttggc
     1741 atcgcccgac acccaggcgg tgccggcgat gtcgaggtgg gcccacttgt aggccttggc
     1801 gaagcgcgac aggaagcagg ccgcggtgat cgtgccggcg taacggccgc cgatgttgcc
     1861 catgtcggcg aagttgctct tgaggagttc ctggtattcg tcccacagcg gcagctgcca
     1921 ggcgcggtca cccgactcgg tgccacggcg caggatctcg gcggcgagct cgtcgtcgtt
     1981 ggcgagcagt ccgctcggga tcttgccgag cgcgaccacg caggcgccgg tgagcgtggc
     2041 gatgtcgatc acgcactcgg gcttgaagcg ctcggcgtag gtgagcgcgt cgcacaggat
     2101 caggcggccc tcggcgtcgg tgttgagcac ctcgatggtc tgccccgaca tcgaagtgac
     2161 gacgtcgccg ggcttggtgg cgccgccgcc gggcatgttc tcggtcgtgg ggacgaggcc
     2221 gaccaggttg atcggcaggc ccatctgcgc gaccgccttg aaggtgccga gcacgctcgc
     2281 ggcgccgcac atgtcgaact tcatctcgtc catctcggct gcgggtttga gcgagatgcc
     2341 gccggtgtcg aaggtgatgc ccttgccgac gagcacgacc ggcttcgcct tggccttgcc
     2401 gcccttgtag tgcatgacaa tgaacttggg cggctggtgc gagccgcgcg ccacggacag
     2461 gaaggagccc atgccgagct tctccatgtc gtcgcgctcg agcacctcga cgtcgaactt
     2521 gtactgcttg cccagggact cggccgtttc cgccaggtgg gtgggggtgc agtggttgcc
     2581 gggcaggttg ccgagatcct tcgccagcgc catgccgctg gcgatcgcgt ggccctgcag
     2641 gacggcggtg tcgagctcgg cgccgagctc gcagctcacc agcaggcaga gcttcttcgc
     2701 gccacgctcc ttccgggtct tcttgtcgga cttgagtgcg tcgaagcggt aggcgccgtc
     2761 ggcgaggatg cgcgcggcct gctgcagccg ccacgccatg tcgcgaccgg agagctctgc
     2821 gtcggcgagc gcgaccacgg cgtccttcgc cggcccggcg gagatcgcct tcgccgcggc
     2881 ggcgagcgcg tcgcggtacg ccttgtcgcc gaactcgtcg tgcttgccca ggctcaccag
     2941 cagcacgcgc tcggcgtgca cgccgggcag gtcatgcacc agcagcgtgg cgcccgcctt
     3001 gtcctcgagg tcgccacgct tgatcagggc ggccaggcgg ccttcggcag caccgtcggc
     3061 cgcggccgag agggcgggga gacggccttc ggcaaacgcg ccgagtacca gcaggccagt
     3121 cttgagcttg gccgggctgc cggtctttat ggtaaattcc atgcgttttt ccttgtttgg
     3181 atgcctggtt tcgattgccg aagattatgg cgcgttcgca accggggcgt caaaaacgct
     3241 tcccatccac atccgccgcg gcctgccgcc cggcctcatc ccggtatcga cccccgcccg
     3301 atgatcttcc ggcgcgccct gcaacgggaa ttcgcgcaga cggccgccgc ggtcttcgtg
     3361 gcgctgttcg cgatcctgat ctccaccgtg ctcatccgcc tgctcggcca ggcggcgggc
     3421 gggcgcgtgc cgcccgatgc cgtggtggcg ctgatcgggt tcggcgcgct tgcgcagctg
     3481 cccacggtgc tgacgctcac cttgttcatc gccatcctgg tgtcgctgtc gcgcagctac
     3541 cgggattccg agatggtggt gtggttcgcc tcgggtgtgc ccctcaccgc ctggatccgg
     3601 ccggtcctga tgttcgcgct gcccgtcgtg ctggtggccg gtggcggctc gctctacctg
     3661 acgccgtggt cacagcagaa gagcgcggaa taccgcgagc gcctggagaa tcgcgacgat
     3721 acccatcgcg tcgcgcccgg cgtcttccgc gagtccgcga gcgcgcagcg ggtgttcttc
     3781 gtcgaggtcg gcgccggcga ggatggccgg gtgcgcaatg tcttcgtcag cgagcagacc
     3841 cgcagcggcc agttggtggt gatcgcctcg gccgaaggct acctgcgcgg cgatgcggac
     3901 ggccggcggt ttgtcgtgct cgagaaaggg cggcgctacg acgggacccc gggtacgccc
     3961 gagtaccggg taatggagtt cgatcgctac gaagtgcagg tcgagcagag gcaggtcgcg
     4021 ggccagcagg cacgcatccg cgcgcttccg accgcggtgc tgctcgcgca gcccgagccg
     4081 cgcggcctcg gcgaactcgt cggccgcttc ggcgtgccgg cggtcgcgtt gctgctggcc
     4141 ttgatggcga tcccgctgtc cttcgtcaat ccgcgcgccg ggcgtgcgaa caacctgctg
     4201 gtggcgctgc tcgtgtacct gctctacagc aacgcgattt ccgtgttcca gtcctgggtt
     4261 gcgcagggca agctgccctt cgtggccgga ttgctgctgc cccacgtggt ggtgctggcg
     4321 gtgctggcgc tgatgttcca caagcgcctg gcgatctcgc cgttctggcg gaggcgtgca
     4381 tgagcgcacc cgcacggccg cccgaaggga ggcgctcggc gccctcccga aggatgtcgc
     4441 ccggcggcag gaggggagtc cggtgagtcc ggttctgctg cgctatctcg cgcgcgagat
     4501 cttcgccgcc agtgcgctgg tgctggtggc ctttctcggc ctgttcgcct tcttcgactt
     4561 catcaacgag ctcgaggacg tgggcaaggg cggctacacg ctctaccagg ccctggtgta
     4621 tgtcggactc accctgccgg cgcgggtgta tgagctgatg ccgatcgcgg tgctgatcgg
     4681 caccctgtat gcgctggcga ccttggcgaa gaactccgag atcaccgtga tgcgagcctc
     4741 ggggatgtcg aacgcgctgc tgacgagcgt gctcggcctg atcggcggag tgttcgccgt
     4801 gctgatcttc ctggtcggcg aatacgtggc accgcccgcg gaggccgccg cggaggagtg
     4861 gaagctcacc gccacgcagg cgaccgtctc gcagcaactg cgctcgggcc tgtgggtgaa
     4921 ggacggccgg ctcgtgctca acgtgcgcac gctgatgccc gaccgcagca tgcgcggcgc
     4981 gcgcatctac gccttcgatg aagactacgc gctcgaatcg atcgccgaag cggcgaccgg
     5041 cgtgttcgac ggcagcgggg agtggaagct gctcgacgtg cagcgtacgc gcttccacgg
     5101 cgatcgcacc gaggtcgagc gcctgcccga aatccgctgg cgttcggagc tgacgccgga
     5161 ggtgctgggg gtgctcatgg tgtcgcccga gcacatgccc ttcggtcgcc tgtggaccta
     5221 catcaaccac ctgcgcgaca acaaccagaa cgccgaccgc ttcgagatcg cgctgtggaa
     5281 gaagctgatc taccccttca ccgcgttggt gatgatggcg ctggcgatgc ccttcggttt
     5341 catccacgac cgcatgggcg gcgcgagcgc gcgcatcttc atgggggtga tgctgggcgt
     5401 gggcttccac ctcctcaacg gcctgttctc caacctcggc atgatcaacg cctggccgcc
     5461 cttcatggct gcgctgacgc ccagcctgat cttcctcgcg gccgcgttct ggctcatgca
     5521 tcgcgtcgaa cggcgctgag cagggggcca cggaggaggc gtcccggcgc gtgtccaggc
     5581 gtcgcaatcg cgactcgcgt cgctcctgca acggactccc agcgccgcag ccgcgccgca
     5641 ggagcgacgc aagtcgcgat cgacgggccg gcgtggccgc gcctgccaag ccccgccgcg
     5701 cggctgcggt gaacgaaaaa acgggcccga aggcccgttt cgatgaagcg aacgccggag
     5761 cgatcaggct tcctgttgct gctgggcggc acgcttggcg gcagcggcct tgtagtccag
     5821 cttttccttg atgcgcgccg acttgcccga gcgctggcgc aggtagtaca gctt
//
LOCUS       DAOQNN010000189         5838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7946_length_5838_cov_11.533979, whole genome shotgun sequence.
ACCESSION   DAOQNN010000189 DAOQNN010000000
VERSION     DAOQNN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7946_length_5838_cov_11.533979"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..428
                     /locus_tag="PKV97_10530"
     CDS             303..428
                     /locus_tag="PKV97_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06354.1"
                     /translation="MCAWPLSLLKKEDMLSTVGYVPSGVVEIMKKQQQGFAYLRP"
     gene            770..1978
                     /locus_tag="PKV97_10535"
     CDS             770..1978
                     /locus_tag="PKV97_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06355.1"
                     /translation="MFTMHVLRTAAISGALTLLMSSQAAQAQVRTDRLQSLGTTQQAV
                     QPLIVNRNTSLADLLAKPDNTVLVMPDGKRTSVAALRNALRTRDETLAALQKTSAAQP
                     TGPRNRQAAGGVRAPGLEMQASLSSSRWGKAVDANTQTRAITVLEEGVHYIVTTQGNR
                     NVSPGDTLTIVGRGFGDTLGEAALVGQFPGGATRLQVLNWRDTEVKTKVPADIRGVLD
                     HDVELQVVTAARRTMPSSKGGYFIAAREEVTLDRGVGRVLRMASKSAPAATMTDDGVV
                     NRWISGPGASTTCWVPSHDDFDIVEPGRGWALTGYWYWTGRQDTGTGDATGHRGWRAF
                     QGEYRPQVNERDPGNRVLTMKFGVWESFSSADPSVLDTYLMNDTSARGCGSWYRIHPI
                     LSGPAGTSPF"
     gene            2204..2599
                     /locus_tag="PKV97_10540"
     CDS             2204..2599
                     /locus_tag="PKV97_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06356.1"
                     /translation="MSRNAGSALLVCAIAAVSVLAASRGGAADVVRNPGVGQAAPSPT
                     EVSLLQTRVAALEKKFDELQREHAALQRQYAVHTHKHSRTASDCTSTNLFALKKRLDS
                     NSGPGMDDRVCLLHPGSGAIEVTTTGPSD"
     gene            complement(2635..3096)
                     /locus_tag="PKV97_10545"
     CDS             complement(2635..3096)
                     /locus_tag="PKV97_10545"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HNB06357.1"
                     /translation="MTFAAACLATSLAQGASAGESGQARKSGLWEISTTIQGQPKPSS
                     ILQCVDERTDDVMGRGGIKDAKCSRNEVKLQGNRITVSSACEIGGTRMTTEGVFTGSF
                     DSAYRAELKTTYDPPMMGMKQATMVIDAKWTGPCKAGQKPGQIVAPHPTKR"
     gene            3269..3871
                     /locus_tag="PKV97_10550"
     CDS             3269..3871
                     /locus_tag="PKV97_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06358.1"
                     /translation="MFGLFKKAPSQRHPNGYTPGLGLGSVPADVVVHWMREARVTGLD
                     DTPRAVRKGRGLALPDGSALAVLSQPLAAGIAGSAELMIRSSEIGGTLLATVRREGEG
                     RYTLDGDAVVGLHFERTVPDAVARTLECPPADFGGIWDLMDADGTIARDIPFFDGGRG
                     QATSTIEIHRQPPPSALTLPLLVLLLLARATLSIPAHPQR"
     gene            3883..5193
                     /locus_tag="PKV97_10555"
     CDS             3883..5193
                     /locus_tag="PKV97_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06359.1"
                     /translation="MKRPQFLRALRVRACTFAVAGFFSLPAQACCTDFWSCAAAVASA
                     GLTCAEEAALKAAHELTELVLRQRDQAVRAVEEAANNDARAAQGVVDQWQRRAEAAAA
                     ETTRAVARAAQLMGDDGTRRAAPLDAMTQTQPMRPGQSNPNSARLARLERSVERTANL
                     GAPPDHSMADVQKQVAAADRQVQDLQRSNAQLSAQSRATIDHAKAEFIAKQVAIVTSG
                     FAALSARFAIGNSNDDKAKLLALAGNVLLVTQFIDQLVLQLDRDLTRDGGAFHQRLMI
                     SADDPRDSALSAESQVLRVREWMAAMERMARLQSLQEREQIARPLLAATPPGGVPPRN
                     VDKISMTLSAPSAINASMRASLARVRDAVQRLPATARRPDVTPMRKQAEDDLQARFRG
                     KSPAEAARLRDEMLAEARRRFGGQPQWLPDVEALIQQAMANRPR"
     gene            complement(5733..5825)
                     /locus_tag="PKV97_10560"
     CDS             complement(5733..5825)
                     /locus_tag="PKV97_10560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEPxxWA-CTERM sorting domain-containing protein"
                     /protein_id="HNB06360.1"
                     /translation="MFTTAVPEPETWAMLLAGLGLMALTARRRR"
BASE COUNT         1015 a         1946 c         1870 g         1007 t
ORIGIN      
        1 tgcgggcgcg tccagacata tccttgaggc catcgagcca atcgcggtag tcatcagtct
       61 tctcgacacg catgttcgta ttgtagcttc caggctacaa gcgccgcaac caaggcggtg
      121 actaagcaca ttggaggcga cgggtggccc gacagccatt gggccatcac gattgaccga
      181 aaactctgcc ggctcgaagc aatccttcaa ctcgggcgac ttggcgttct gagccgccac
      241 gtcacggagg aatacgcaat agctgggtat ggggaggtag gtctatccgg ttcaggcaaa
      301 gaatgtgcgc ctggcccctt tccctgctca agaaggaaga catgctctcc accgtgggct
      361 acgtgccctc cggcgtggtg gagatcatga agaagcagca gcaggggttc gcgtacctgc
      421 ggccctgagt ttgtgcagtg tgacgtaaat cgcatcggcg cgtcctgcct actcggtgcg
      481 ggttgctgag ggtggttggc cgggggtggc ccgcggccag ttactgtctt tgtttggaca
      541 aagaaagccc tcctcccttc caccgactac ctcgcctggt tgtccaaagc ctgttccatg
      601 gttggcctca cgcggttgaa tgccctgttc gttgtcggcg gttgcgccaa tcctgggcag
      661 gatgctagcc tgaaccgagg taccaaaatg ctcttcgatt acaccagccg ccgcttgctg
      721 cggcggctgg tgtcaacttg gcgcagcatc accggaggag aacaatacga tgttcacgat
      781 gcacgtgctg cgcactgctg cgatctctgg ggccttgacc ctgctcatga gttcgcaggc
      841 cgcacaggct caggtgcgca ctgaccgcct tcaatctctg ggcacgacgc aacaggcagt
      901 ccaacccctt atcgtcaatc gaaacacgtc gctggccgat ctgctcgcca agcccgacaa
      961 caccgtgctc gtgatgcccg atggcaagag aacctcagtg gcggcattgc gcaacgccct
     1021 acgaacgcgc gacgaaactc tggccgctct gcagaagacc agcgcggccc agcccaccgg
     1081 cccacgcaac cgccaggccg ccggtggtgt ccgtgcgcct ggcctcgaaa tgcaggcctc
     1141 gctcagcagt tcgcgctggg gcaaggccgt cgatgccaat acgcagacgc gcgcgatcac
     1201 ggtgctcgaa gaaggcgtgc actacattgt caccactcag ggcaaccgca atgtgtcccc
     1261 cggcgatacg ctcaccattg tgggccgcgg cttcggcgac acgctgggcg aggcggcgct
     1321 ggtgggtcag tttccaggcg gcgcgacccg gctgcaggtg ctcaactggc gcgacaccga
     1381 ggtcaaaacc aaggtgccgg ccgacatccg tggcgtgctg gatcatgacg tcgaactaca
     1441 ggtcgtgacc gctgcgcgcc gcaccatgcc gtctagcaag ggcggctact tcatcgccgc
     1501 gcgcgaggag gtgacgctcg atcgcggtgt tgggcgcgtg ttgcgcatgg cgagcaagag
     1561 cgctcccgcg gccaccatga cggacgatgg cgtcgtcaat cgatggatct cgggccccgg
     1621 cgccagcacg acgtgttggg ttccgagcca cgacgacttc gacatcgtcg aacccggccg
     1681 tgggtgggcg ttgaccggct actggtactg gaccggccgg caggacaccg gcaccggcga
     1741 tgcaaccggc catcgaggct ggcgcgcttt ccagggcgag taccgccccc aagtcaacga
     1801 gcgcgaccct ggcaaccggg tgcttaccat gaagttcggc gtctgggaga gcttctcatc
     1861 cgccgaccca agcgtcctcg acacctatct catgaacgac acctccgccc gcggctgcgg
     1921 ctcctggtat cggatccatc ccatcctatc cggccctgcg ggcacttcgc cgttctgatc
     1981 tcctaaggag aacgttcacg gatcgtcttg tcccaccatc gactcgcatc ccgctagctc
     2041 tggccattgc gctgctcgtc agtactcggc ccaggcagcg acctactcgc cctgtgtctt
     2101 tgatagtcag ccgctcaaga ggacggggtg tggagcacac cgtagtccct cgagctcacg
     2161 ctgtcgcttt gaaaccaaca ttgctgatcg aaaggagccc gatatgagtc ggaacgctgg
     2221 aagtgcactt ctggtctgcg cgatcgcggc agtcagcgta cttgcggcct cgcggggcgg
     2281 tgcggcagat gtggtaagaa accccggtgt gggacaggcc gccccgagcc cgaccgaagt
     2341 atctcttctc caaacgcgcg tcgccgcgct ggagaagaag ttcgatgagc ttcaacgcga
     2401 acacgccgcg ctgcagcggc agtacgcggt gcacacgcat aaacattcac gcaccgcttc
     2461 ggattgcaca tcgacaaacc tgtttgccct gaaaaagcgg ctcgactcga attcaggccc
     2521 gggaatggac gaccgggtct gtctcctcca tccgggctcg ggcgcgatcg aggtcacaac
     2581 gacaggcccg tcggactgat caatcgaccg gagctgccac aaaccgaccg ccgctcaacg
     2641 tttggtcggg tgcggcgcca cgatctggcc cggtttctgg cccgccttgc acgggccggt
     2701 ccatttcgcg tcgatcacca tcgtggcctg cttcatgccc atcatcggcg ggtcgtaagt
     2761 ggtcttcaat tcggcgcggt aggcgctgtc gaagctgccg gtaaacacgc cctcggtggt
     2821 catgcgcgta ccgccgattt cgcaggccga gctcaccgtg atgcggtttc cctgtagctt
     2881 gacctcgttg cgcgaacact tcgcgtcctt gatgccaccg cggcccatca cgtcgtcggt
     2941 cctctcgtcc acgcattgca ggatcgagct cggcttgggt tggccctgaa tcgtggtgct
     3001 gatttcccac agaccggact tgcgggcctg gcccgactcg ccggccgatg cgccttgagc
     3061 gagactcgtt gccaagcaag cggcagcgaa ggtcactctt gccattgatc cttgatacga
     3121 atgcatcggc tgtcccccgt atgtacaagc tccgttgtgg cctggcatta tcgaggccgg
     3181 gtgggaaggg ggtcaaacac ccagttcaca tggcggcgcg tacacgccgc gtcctcaaca
     3241 gaatccactt cgtcgaggag cccagcgtat gttcggcttg ttcaagaagg caccttcgca
     3301 gcggcacccc aacggctaca ccccgggcct cggcctgggc agcgtgcctg cggatgtggt
     3361 ggtgcattgg atgcgcgagg cgcgcgtcac cgggctcgac gacacgccgc gcgccgtgcg
     3421 caagggccga ggcctcgcct tgcccgacgg cagcgcgctc gcggtgttgt cgcagccgct
     3481 ggccgccggg atcgcgggca gcgccgagct catgatccgc tcgtcggaga tcggcggcac
     3541 attgctcgcc acggtgaggc gtgaaggcga aggccgctac acgctcgatg gcgacgcggt
     3601 ggtcggcttg cacttcgagc gcaccgtacc cgacgcggtg gcacgtacat tggagtgtcc
     3661 gcccgccgac ttcggcggca tatgggatct gatggatgcc gacggcacga tcgcccgcga
     3721 cattcccttc ttcgacggcg gtcgcggcca agccaccagt accatcgaga ttcaccgcca
     3781 accgccgccc tctgccctga cgctgccctt gctggtgctg cttctgttgg cgcgggcaac
     3841 cctgtcgatc ccggcgcacc cgcaacgctg accaggccat ccatgaagcg cccccagttc
     3901 ttgcgcgccc ttcgcgtccg agcgtgcacg ttcgccgtgg ccggcttttt cagcctgccg
     3961 gcccaggcgt gttgcaccga cttctggagt tgcgccgccg ccgtggcatc ggccgggctc
     4021 acctgtgcgg aggaggcagc cctgaaggcg gcgcatgagc tcaccgagct cgtgctgcgc
     4081 cagcgcgacc aggcggtccg tgcggtggag gaagcggcca acaacgatgc gcgcgcggcg
     4141 cagggtgtgg tcgatcaatg gcagcgtcgc gccgaggctg ccgcagccga aaccacccga
     4201 gccgtcgcgc gcgccgcgca gctgatgggc gacgacggca cacgccgcgc agccccactc
     4261 gatgccatga cgcagacgca gccgatgcga cctggccaga gcaatcccaa cagtgcccga
     4321 ttggcccgcc tggagcgctc cgtcgagcgc acggccaacc tcggcgcacc tcccgaccac
     4381 tccatggccg atgtgcaaaa gcaggtagcc gccgccgacc gccaagtgca ggatcttcaa
     4441 cgcagcaatg cgcaattgtc cgcgcagtct cgcgccacga ttgaccacgc caaggccgag
     4501 ttcatcgcca agcaagtggc catcgtcacc agtggctttg ccgccctcag cgcgaggttc
     4561 gccatcggca acagcaacga cgacaaggcc aagctattgg ccttggccgg caatgtgctg
     4621 ctcgtgacgc agttcatcga ccaactcgtg ctgcagctcg accgcgacct gacccgggat
     4681 ggcggtgctt ttcatcagcg cctgatgatc agtgccgacg acccgcgcga cagcgccctc
     4741 agcgccgaaa gccaagtgct ccgcgtgcgc gagtggatgg ccgcgatgga acgcatggcg
     4801 cgccttcaga gcctgcagga gcgcgagcag atcgcgcgac cgctgcttgc cgcgacgccc
     4861 cccggcggtg tgccgccgcg caacgtggac aagatttcga tgacgctgtc cgcgccgtcg
     4921 gccatcaacg cttcaatgcg cgcgagcctg gcccgggtgc gggacgcggt gcagcgccta
     4981 cccgccaccg cgcgccggcc tgacgtgacg ccgatgcgca agcaggccga ggacgatctg
     5041 caagcccgct tccgtggcaa atccccagcc gaggccgcgc ggctacgcga cgagatgctc
     5101 gccgaagcgc gccgccgctt tggcggtcaa ccgcaatggt tgcccgacgt ggaagcgctg
     5161 atccagcaag ccatggcgaa ccggccgcgg taaggccggt ccggcatagc cgttcgacgt
     5221 cgacatgccc acgggcggcg cacaagcctc gtcgccaccg gcgccggccg gctgtgcaaa
     5281 gccggccgtg ggtggtcgct tgggcgagga cgattcttgg ggtggattga atctgcgacg
     5341 taaggggtgt ggcattggcc gggggtggcc cggcggcgag ttacttttct tgcttggcca
     5401 agataagtaa ccaaaagaag gccaccccgc ttccgccgaa atccccgcgt ccccccattc
     5461 ccacgggcgg ctcgcaaact cgccctgcgg gctcaaacat cgcttgccga cggcccccgt
     5521 gggaatgggc gagacgcggc ggcggcagag gggaaaggag gggtttcggt aagcatgcct
     5581 cacttccgtt gaagccagtc ggctgtgcaa agccggccgt gggaggaacg tgcaagcgaa
     5641 gggcggaaag cgcttcgctt ttccgccctt cgcgggctag taggtctgtt acggcgggaa
     5701 acggcccctc gcggaggggc cgccgggtcg tttcagcggc gtctgcgcgc cgtcagtgcc
     5761 atcaggccca ggccggcgag cagcatggcc caggtttccg gctcgggtac ggcggtggtg
     5821 aacaagacgt tctggggc
//
LOCUS       DAOQNN010000190         5817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_7987_length_5817_cov_10.092676, whole genome shotgun sequence.
ACCESSION   DAOQNN010000190 DAOQNN010000000
VERSION     DAOQNN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7987_length_5817_cov_10.092676"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PKV97_10565"
     CDS             <1..517
                     /locus_tag="PKV97_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB06361.1"
                     /translation="PAPVPRLEAPPPARVERAQRPEPVVGEVRDAASATPTGVRFNQL
                     RGQLRFPVRGELVGRFGAPRAEGGTTWKGVFIRAGNGAEVRSVAGGEVVFSDWLRGYG
                     NLIIVDHGSDYLTIYGNNDSLLKEVGDRVGGGEPIASVGAAGVGNDSGLYFEIRHQGQ
                     PLDPMQWMRLN"
     gene            562..2013
                     /locus_tag="PKV97_10570"
     CDS             562..2013
                     /locus_tag="PKV97_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNB06362.1"
                     /translation="MRSSKLKQFGLVMTGMVAGIMISLNFSANADRSAPLPLPVDELR
                     AYADVFNAIKQGYVEPVEDKKLITDSISGMLSGLDPHSAYLDAEAFKDLQVGTQGEFG
                     GLGIEVGMEDGFVKVISPIEDTPAFRAGVLAGDLIVKLDDTPVKGMSLNDAVKRMRGK
                     PRTDIILTIMRKGQSQPIVIKLTREVIKVQSVKSKVVEPGYAYLRIAQFQENTAASVV
                     EHLAKLGAQGPVQGLVLDLRNDPGGLLHGAVGVSAAFLPEETLVVSTDGRTEDAKREY
                     RAIQADYLRGTRDDFLRRLPAYARDVPMVVLVNAGSASASEIVAGALQDHKRARVIGT
                     QTFGKGSVQTILPLNNNTAIKLTTARYYTPSGRSIQAKGIAPDIVVEETANGSGRRVR
                     EADLLGHLENDKGAVEAAKAAAGKADGAPAEETAAETARFELAGDNDHQLKQAIRLLK
                     GEDIAHAAPAVEAEAAGGQAAAAGAAQPAAAKQ"
     gene            2041..2490
                     /locus_tag="PKV97_10575"
     CDS             2041..2490
                     /locus_tag="PKV97_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06363.1"
                     /translation="MRRETAYKLAGRHHDRPLPGADIHKHREIRFKPLPPGQVERAWK
                     SLQLLKGLQLERTDDPLGIVVRYSVLDYSLESLEDALREAGFALENSLYIKLVRALVY
                     FSEETQRHNLLSPERLIKQSNEVYIQAWDHHVHGDHDDTPLELREYK"
     gene            2498..3280
                     /gene="moeB"
                     /locus_tag="PKV97_10580"
     CDS             2498..3280
                     /gene="moeB"
                     /locus_tag="PKV97_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNB06364.1"
                     /translation="MNDDQLLRYSRHILLPEIDVEGQQALLDARALVVGAGGLGSPAA
                     MYLAAAGVGTLVLADHDTVDLTNLQRQIMHSADMVGLPKVASARATLHRLNPQTRLEP
                     IQARLEGEALEAEVGRADVVLDCTDNFATRHAINRACVKHRKPLVSGAAIRFDGQVSV
                     FDLRKPDSGCYHCLFPEGEDIEEVRCAVMGVFAPIVGIVGTIQAAEALKVLIGCGRTL
                     DGRLLLLDGLSMEWRSVALGRDPGCAVCGTARCAGAVESALR"
     gene            complement(3266..4363)
                     /gene="ribD"
                     /locus_tag="PKV97_10585"
     CDS             complement(3266..4363)
                     /gene="ribD"
                     /locus_tag="PKV97_10585"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNB06365.1"
                     /translation="MTFSAADHAAMARALQLAARGLDTTSPNPRVGCVIVRDGTTVGE
                     GWHARAGEAHAEVHALRQAGAAARGATAYVTLEPCSHFGRTPPCADALIEAGVARVIA
                     AMEDPNPLVAGRGLARLRASGIAAAHGLLAEAAQELNIGFVSRMTRGRPWVRLKVAGT
                     LDGKSALDNGVSQWITGPAARRDGHRWRARACAILTGIGTVREDDPQLGVRDVPCERQ
                     PLRILVDARLEVSPQARILQGEPILIATGSTDQARIDMLRAAGHEIVFVPNAAGKVDL
                     GALLRLLGERGINELHVEAGFKLNGSLLREGCVDELLIYLAPMLVGDAAQGLFNLPAL
                     TRLEGATRLDIRDLRRIGPDLRLIARPLAQG"
     gene            complement(4344..4856)
                     /gene="nrdR"
                     /locus_tag="PKV97_10590"
     CDS             complement(4344..4856)
                     /gene="nrdR"
                     /locus_tag="PKV97_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNB06366.1"
                     /translation="MKCPFCADPNTQVTDTRENEDGDVVRRRRRCVKCDKRFTTYERI
                     DLKMPHIVKRNGNRTEFDHAKLAGSMKLALRKRPVTIEAIDAAVDRIEARLLAMGEQE
                     VPSEKIGELVMKELKKLDKVAYIRFASVYRNFADVDEFAEVIREVQTRPRRGKADKGG
                     ADPGHDLFGG"
     gene            complement(4917..>5817)
                     /gene="glyA"
                     /locus_tag="PKV97_10595"
     CDS             complement(4917..>5817)
                     /gene="glyA"
                     /locus_tag="PKV97_10595"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNB06367.1"
                     /translation="GMSLAEGGHLTHGMPLNMSGKWFNVVAYGLDAKEEIDYDKMEAL
                     AREHKPKIIIAGASAYALRIDFERFAKIAKEVGAIFWVDMAHYAGLIAAGFYPNPVPH
                     ADVVTSTTHKTLRGPRGGIILMKAEHEKALNSAIFPGLQGGPLMHVIAAKAVAFKEAA
                     SPAFRDYQEQVIANARVMARVLSEERGLRIVSGRTESHVFLVDLRNKKITGKAAEAVL
                     GSAHITVNKNSIPNDPEKPFTTSGIRLGSPAMTTRGFTEIEAEKIAHLIADVLDAPQD
                     ETVIARVRGQVAELCAKFPVYGK"
BASE COUNT          881 a         1972 c         2074 g          890 t
ORIGIN      
        1 gccggcgccg gtgccacgcc tcgaggcgcc gccgccggca cgggtggagc gtgcgcagcg
       61 ccccgagccg gtggtcggcg aggtccgcga cgccgccagt gcgacgccca ccggcgtgcg
      121 cttcaaccag ctgcgcgggc agctgcgctt tccggtgcgc ggagaactcg tgggccgctt
      181 cggtgctcca agggcggagg gtggaaccac ctggaagggc gtgttcatcc gcgccggcaa
      241 cggtgccgaa gtgcgttcgg tggcgggcgg ggaagtggtc ttctcggact ggttgcgcgg
      301 ttacggcaac ctcatcatcg tcgaccacgg cagcgactac ctgaccatct acggcaacaa
      361 cgattccctg ctcaaggaag tgggcgatcg cgtcggcggt ggcgagccga tcgccagcgt
      421 gggcgccgcc ggggtgggca acgattcggg tttatacttc gagatccgtc accaggggca
      481 gccgctcgat ccgatgcaat ggatgcggct caactagacg ggacggccga ccggggcggt
      541 cggcgcacta ctggagttct catgcgcagc agcaagctga aacagttcgg cctcgtcatg
      601 accggcatgg tggccgggat catgatcagc ctcaatttct ccgccaacgc cgaccgcagc
      661 gcgcccctgc cgctgccggt cgacgagttg cgcgcctatg ccgacgtctt caacgcgatc
      721 aagcaaggct acgtcgagcc ggtggaggac aagaagctga tcaccgactc gatcagcggc
      781 atgctctccg ggctggatcc gcactccgcc tatctcgacg ccgaggcctt caaggacctc
      841 caggtcggca cccagggcga attcggcggt ctcggcatcg aggtcggcat ggaagacggc
      901 ttcgtcaagg tgatctcgcc gatcgaggat actccggcct tccgcgccgg tgtgctggcg
      961 ggcgacctga tcgtcaagct ggacgacacc ccggtcaagg gcatgagcct caacgacgcg
     1021 gtcaagcgca tgcgcggcaa gccgcgcacc gacatcatcc tcacgatcat gcgcaagggg
     1081 cagtcgcagc cgatcgtgat caagctgacg cgcgaagtca tcaaggtgca gagcgtcaaa
     1141 tccaaggtgg tggagcccgg ctacgcctac ctgcgcatcg cgcagttcca ggagaacacc
     1201 gccgcgtcgg tggtcgagca cctggccaag ctcggcgctc agggcccggt gcagggcctg
     1261 gtactcgacc tgcgcaacga ccctggcggc ctgctgcacg gcgccgtggg cgtgtcggcg
     1321 gccttcctgc ccgaagagac cctcgtggtg agcaccgacg gccgcaccga ggacgccaag
     1381 cgcgagtacc gcgccatcca ggccgactac ctgcgcggca cccgcgacga cttcctgcgc
     1441 cgcctgccgg cctatgcgcg cgacgtgccg atggtggtgc tggtgaacgc gggctcggcc
     1501 tcggcctcgg agatcgtcgc cggcgcgctg caggatcaca agcgcgccag ggtgatcggc
     1561 acgcagacct tcggcaaggg ctcggtgcag accatcctgc cgctcaacaa caacaccgcg
     1621 atcaagctca ccaccgcacg ctactacacg ccgagcgggc gctcgatcca ggccaagggc
     1681 atcgcgcccg acatcgtcgt cgaggagacc gccaacgggt ccggccgccg cgtgcgcgag
     1741 gccgatctgc tggggcatct cgagaacgac aagggcgccg tggaggccgc gaaggccgct
     1801 gccggcaagg cggacggcgc gccggcagaa gaaaccgcgg ccgagactgc gcgcttcgag
     1861 ctcgcgggcg acaacgacca ccagctcaag caggcgatcc gcctgctcaa gggcgaggac
     1921 atcgcccacg ccgcgccggc cgtcgaggcc gaagcggccg ggggacaggc cgcggcggcc
     1981 ggggccgcgc agcctgcggc ggcgaaacag taagccggga gtccggccgg ggggacgatc
     2041 atgcgcaggg aaactgccta caagctggcg ggccggcatc acgaccggcc cttgccgggc
     2101 gccgatatcc acaagcatcg cgagatccgc ttcaagccgc tcccgcccgg gcaggtggag
     2161 cgcgcctgga agtcgctgca actgctcaag ggcctgcagc tcgagcgcac cgacgatccg
     2221 ctgggcatcg tggtgcgcta ctcggtgctc gactattcgc tcgagtcgct cgaggacgcg
     2281 ctgcgcgagg ccggctttgc gctggagaat tcgctctaca tcaagctggt gcgggcactc
     2341 gtgtatttca gcgaggagac ccagcgccac aacctgctgt cgcccgaacg cctgatcaag
     2401 cagtccaacg aggtctacat ccaggcctgg gaccaccacg tgcatggcga ccacgacgac
     2461 accccgctcg aactgcgcga atacaagtaa tcgggcgatg aacgacgacc agctgctgcg
     2521 ctacagccgc cacatcctgc tgcccgagat cgacgtcgaa ggccagcagg ccctgctcga
     2581 cgcgcgcgcg ctggtggtcg gcgcgggcgg gctgggctcg cccgcggcga tgtaccttgc
     2641 cgccgccggg gtcggcaccc tcgtgctcgc cgaccacgat acggtcgatc tcaccaacct
     2701 gcagcgccag atcatgcact cggccgacat ggtcggcctg cccaaggtcg cctcggcacg
     2761 tgccacgctg caccggctca acccgcagac ccggctcgag ccgatccagg cgcgcctgga
     2821 gggcgaggca ctcgaggccg aggtggggcg cgccgacgtg gtgctcgact gcaccgacaa
     2881 cttcgccacc cgccacgcca tcaatcgcgc ctgcgtgaag caccgcaagc cgctggtgtc
     2941 gggcgcggcg atccgcttcg acggtcaggt ctcggtgttc gacctgcgca agcccgacag
     3001 cggctgctac cactgcctgt tcccggaagg cgaggacatc gaggaggtgc gctgtgcggt
     3061 gatgggcgtg ttcgccccca tcgtcggcat cgtcggcacc atccaggctg ccgaggcgct
     3121 caaggtcctc attggttgcg ggcgcacgct cgacggccgc ttgctgctgc tcgacggctt
     3181 gtcgatggaa tggcgcagtg tcgcgctcgg gcgcgacccc ggctgcgcgg tgtgcgggac
     3241 ggcgcgatgc gccggagcgg tggaatcagc cctgcgctag cggtcgcgcg atcaggcgca
     3301 ggtcggggcc gatgcggcgc aggtcgcgga tgtccagcct tgtggcgcct tccaggcggg
     3361 tgagcgcggg caggttgaag agcccctgcg cggcgtcgcc gaccagcatc ggcgccaggt
     3421 agatcagcag ctcgtccacg cagccctcgc gcagcagcga gccgttgagc ttgaagccgg
     3481 cctcgacgtg cagctcgttg atgccgcgct cgcccagcag gcgcagcagc gcacccagat
     3541 ccaccttgcc ggccgcgttg ggcacgaaga cgatctcgtg gccggccgcg cgcagcatgt
     3601 cgatgcgggc ttgatccgtg ctgccggtgg cgatcaggat gggttcgccc tgcaggatgc
     3661 gggcctgcgg cgacacctcc aggcgcgcat cgaccaggat gcgcagcggc tggcgttcgc
     3721 agggcacgtc gcgcacgccg agttgcggat cgtcctcgcg caccgtgccg atgccggtca
     3781 ggatcgcgca tgcgcgcgcg cgccagcggt ggccgtcgcg gcgtgcggcc gggccggtga
     3841 tccactggct gacgccgttg tcgagcgcgc tcttgccgtc gagcgtgccg gcgaccttga
     3901 gccgcaccca gggccgaccg cgcgtcatgc gtgaaacgaa gccgatgttg agctcttgcg
     3961 ccgcctcggc gagcaggccg tgcgcggcgg cgatgccgga agcgcgcagg cgggcgagac
     4021 cgcgcccggc gacgagcggg ttggggtctt ccatggcggc gatcacgcgc gccacgccgg
     4081 cctcgatcag cgcgtcggcg cagggtgggg tgcggccgaa gtgcgagcac ggctccagag
     4141 tgacgtaggc ggtggcaccg cgcgcagccg ctccggcctg gcgcagcgcg tggacttcgg
     4201 cgtgggcctc gccggcgcgc gcgtgccagc cctcgccgac ggtcgtgccg tcgcgcacga
     4261 tcacacagcc cacgcgcggg ttgggcgagg tggtgtcgag gccgcgggcg gcgagctgca
     4321 gcgcgcgcgc catggcggcg tggtcagccg ccgaaaaggt catgtccggg gtccgctccg
     4381 cccttgtcgg ccttgccgcg ccgcgggcgc gtctgcacct cgcggatcac ctcggcgaac
     4441 tcgtcgacgt cggcgaagtt gcgatacacc gacgcgaagc ggatgtaggc caccttgtcg
     4501 agcttcttga gttccttcat gaccagctcg ccgatcttct cgctcgggac ttcctgttcg
     4561 cccatggcga gcaggcgcgc ctcgatgcgg tccaccgcag cgtcgatggc ctcgatggtg
     4621 accgggcgct tgcgtagcgc cagcttcatg ctgccggcga gcttggcgtg gtcgaactcg
     4681 gtgcggttgc cgttgcgctt gacgatgtga ggcatcttga ggtcgatgcg ctcatacgtg
     4741 gtgaagcgct tgtcgcactt gacgcagcga cggcgacgcc ggacgacatc gccgtcctcg
     4801 ttctcgcgcg tgtcggtgac ctgggtgttg gggtcagcgc agaaggggca tttcatggcc
     4861 ggaaggcggc gccgggcggg gcgggtgcca ttgcggcgcc cgctccgccg aacggcttac
     4921 ttgccgtaga ccgggaactt cgcgcacagc tcggccacct ggccgcgcac gcgcgcgatc
     4981 accgtctcgt cctgcggtgc gtcgagcacg tcggcgatca ggtgggcgat cttctcggcc
     5041 tcgatctcgg tgaagccgcg cgtggtcatc gccggcgagc cgaggcggat gcccgaggtg
     5101 gtgaagggct tctcggggtc gttcgggatc gagttcttgt tcaccgtgat gtgggcgctg
     5161 cccagcaccg cctcggcggc cttgccggtg atcttcttgt tgcgcaggtc gaccaggaag
     5221 acgtggctct cggtgcggcc ggagacgatg cgcaggccgc gctcctcgga cagcacgcgt
     5281 gccatcacgc gggcgttggc gatcacctgc tcctggtagt cgcggaacgc gggcgaggcc
     5341 gcctccttga aggccaccgc cttggccgcg atgacgtgca tcagcgggcc gccctgcagg
     5401 ccggggaaga tcgccgagtt gagcgccttc tcgtgctcgg ccttcatcag gatgatgccg
     5461 ccgcgcgggc cgcgcagggt cttgtgggtg gtcgaggtga cgacgtcggc gtgcggcacc
     5521 gggttggggt agaagcccgc ggcgatgagg ccggcgtagt gcgccatgtc gacccagaag
     5581 atcgcgccga cttccttggc gatcttcgcg aagcgctcga agtcgatgcg cagcgcgtag
     5641 gccgaggcgc ccgcgatgat gatcttcggc ttgtgctcgc gcgccagcgc ttccatcttg
     5701 tcgtagtcga tctcttcctt ggcgtcgaga ccgtaggcga ccacgttgaa ccacttgccg
     5761 gacatgttca gcggcatgcc gtgggtgagg tggccgcctt cggccaggct catgccc
//
LOCUS       DAOQNN010000191         5784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8061_length_5784_cov_5.628906, whole genome shotgun sequence.
ACCESSION   DAOQNN010000191 DAOQNN010000000
VERSION     DAOQNN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8061_length_5784_cov_5.628906"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="PKV97_10600"
     CDS             complement(<1..1907)
                     /locus_tag="PKV97_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNB06368.1"
                     /translation="MSHRLDPACFPLRGSRLIEASAGTGKTWTIAALYLRLVLGHGDA
                     DTAFARPLLPAEILVMTFTRAATRELSERIRERLREAAAWFHNEDAGRAPDPLLDELR
                     AAYPDAAARRNAAWRLAMAAAAMDDAAVFTIDAWCQRMLREHAFDSGNPFDEELAADE
                     SVLLTEAAQDYWRQHCYPLAGEALELVLAQWADVHALVEDMRALVGQPLQGADRDDGG
                     DRDRSADPGDQDGVRRDSAGGETLAALIGRVGGAHRRARVALSRGWAERARAMKDWLD
                     EELATKAKLWNRTKLHERYYTPWLEALEQWARNPLDRRLELPDSARHRLTPDGLLEAR
                     KADAPAPVLPEHFAAFAGLIEDHDALPDPRTALRIHAATRVGERLAELKRQARSFGFA
                     DLLERLDRALAGPHGARLRERILTQYPVALIDEFQDTSPLQYRLFDALYRTADDDPRS
                     ALLLIGDPKQSIYGFRGADIYSYLQARRATAGRHYMLGTNFRSARALVGAVNTWFAQA
                     EDRPGAGAFGFRDAADNALPFEAVAAKGREEVFHADGGVPPALLIHHDLTLRSAGEIR
                     RLFAARCAERIVGWLNDEHAGFQAPGKAFQRLRPADIAVLVRSGREAAEVRRALRRRG
                     LASVYLSDKDSV"
     gene            complement(1904..5500)
                     /gene="recC"
                     /locus_tag="PKV97_10605"
     CDS             complement(1904..5500)
                     /gene="recC"
                     /locus_tag="PKV97_10605"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNB06369.1"
                     /translation="MTAPAPAASLPPGFIALHGNRIETLLDTVAGWLAQHPLAPLESE
                     VILVQSNGMAEWVKMAIARRAGVCAAAAVQLPARFQWQTYRQVLGRAQVPARSPLDKT
                     ALAWRLMRLLPEVLAEPGFAPVAGYLADNDPGRRFQLALRIADLYDQYQVHRPDWLDT
                     WGEGREELLDAGRIATPLPEDQRWQAQLWQRVLDELEPQQRALTRPQLHDRVLATLEA
                     TAEDTALRPAAALPRRVVLFGMTHVPLPTLQLLAALSRHAQVLLAIPNPCRFHWADIL
                     DGREWLRIARRRQPLRDGRELQDVGLDAMHLHAHPLLAAWGRQARDFVRQLDAFDDAE
                     QTRARFPLARIDLFDDGEDAHDGAAPALLVQVQRHIRDLTPLAEHPHPPVPAEDRSIV
                     FHLAHSPVRELEILHDQLLALLARQDAGKPLRPRDIVVMVPSIDDFAPAIRAVFGQYG
                     RHDPRHIPFDIADLSARASSPLMGAVEWLLRIRHDRCRLSDLGALLDVPAIAARFGLA
                     EEGLPRLTGWMRGAGIRWGLDARHRAGLGLQACGEQNSALFGLRRMLMGYAAGRSEVT
                     MDAPFAGIEPYDEVGGLDAELAGALAHLVERLIRWQADSRRPATPAAWTQRLRALLAD
                     LVRAVDDTDRQTLAALDDALERWLEACDEAAFEAELPLAVAAEAWLAALESPALDKRF
                     RAGGVTFCTLMPMRAIPFEVVCLLGMNDGDYPRRSPRSDFDLMALPGQQRPGDRARRD
                     DDRQLMLEALLSARRVLYLSWCGRNVRDNSVQPPSVLVAQLRDYLAAGWSPAVVAERT
                     VEHPLQPFSRRYFEPGTPLLTYAREWRAAHVEAAAQDDAVPSHDGQANREATGRATRP
                     TRGEAASPPLPPFVPDPAVPLTIAALAAFLRNPVKVFFRERLGVVYEDVDEALPDDEA
                     FGFDGLEEHGLVEELAAAVLAELALLPPPELHAFALEAAVEAQLGRIERAGRLPIGGL
                     GLRARAALGEAVLPLLRAWRLVRASHPLDAERRNLRFAHAGALMEDWLDHRVRADEHA
                     LPSWLAHDPGRLLEDADKGTLRPWRLLLPWVRSLLAAAADSPSGGLLVGRDATVHIVP
                     TDPAVARALLARLIEAWIAGLGEPLPVAARTALALVGEGKPAEAYEGSEHRRGEVEEA
                     SLARCYPDYAALIEDGRFEHLARALYGPLLEWMDSHVEAVPHPAPETQESAR"
BASE COUNT          726 a         1935 c         2364 g          759 t
ORIGIN      
        1 accgagtcct tgtccgacag atagaccgag gccaggccgc gccggcgcag cgcgcgccgc
       61 acctccgcgg cctcgcgccc gctgcgcacg agcaccgcga tgtcggccgg gcgcaggcgc
      121 tggaatgcct tgccgggggc ctggaagccg gcgtgctcgt cgttgagcca gccgacgatg
      181 cgctcggcgc agcgcgcggc gaacaggcgg cggatctcgc cggcgctgcg cagcgtcagg
      241 tcatggtgga tgagcagcgc cgggggcacg ccgccatcgg catggaagac ctcttcgcgc
      301 cccttcgccg ccaccgcctc gaagggcagg gcgttgtccg ccgcgtcgcg aaagccgaag
      361 gcgcccgcgc ccgggcggtc ctcggcctgg gcgaaccagg tgttgaccgc gcccaccagc
      421 gcgcgcgccg agcggaagtt ggtgccgagc atgtagtgcc ggccggcggt ggcgcggcgg
      481 gcctgcaggt agctgtagat gtccgcgccg cgaaagccgt agatcgactg cttggggtcg
      541 ccgatcagca gcagcgcgct gcgcgggtcg tcgtcggcgg tgcggtagag cgcgtcgaag
      601 aggcggtact gcagcggcga ggtgtcctgg aactcgtcga tcagcgccac cgggtactgg
      661 gtgaggatgc gctcgcgcag gcgcgcgccg tgcggcccgg cgagggcgcg gtcgagacgt
      721 tcgagcaggt cggcgaagcc gaagctgcgc gcctggcgct tgagctcggc gaggcgctcg
      781 ccgacccggg tggcggcgtg gatgcgtagc gcggtgcgcg ggtcgggtag ggcgtcgtgg
      841 tcttcgatca ggccggcgaa ggcggcgaag tgctcgggca gcacgggcgc cggggcgtcg
      901 gccttgcgcg cctcgagcag gccgtctggg gtgaggcggt ggcgggcgct gtcggggagc
      961 tcgagcctgc ggtcgagcgg gttgcgcgcc cactgttcga gcgcctccag ccagggcgtg
     1021 taatagcgct cgtgcagctt ggtgcgattc cacagcttgg ccttggtggc gagttcctcg
     1081 tccagccagt ccttcatggc gcgggcgcgc tcggcccagc cacgcgagag cgccacccgc
     1141 gcccggcggt gcgcgccgcc gacgcgcccg atcagcgcgg ccagggtttc gccaccggcc
     1201 gaatcgcgcc gcacgccgtc ctggtcgccg ggatccgcgc tccgatcccg gtccccgccg
     1261 tcatcccgat ccgcgccctg cagcggttgg ccgacgagcg cgcgcatgtc ctcgacgagg
     1321 gcgtgcacgt cggcccactg cgcgagtacg agctccagcg cctcgccggc gagcgggtag
     1381 cagtgctggc gccagtagtc ctgcgcggcc tcggtcagca gcacgctctc gtcggcggcg
     1441 agctcctcgt cgaaggggtt gccgctgtcg aaggcgtgtt cgcgcagcat gcgttggcac
     1501 caggcgtcga tggtgaacac cgccgcgtcg tccatggccg cggccgccat cgccaggcgc
     1561 caggcggcgt tgcggcgcgc ggccgcgtcg gggtaggcag cgcgcagttc gtcgagcagg
     1621 gggtcgggtg cgcggcccgc atcttcgttg tggaaccagg ccgcagcctc gcgcaggcgc
     1681 tcgcggatgc gctcggagag ctcgcgcgtg gcggcgcggg tgaaggtcat caccaggatc
     1741 tcggcgggca gcagcggacg cgcgaaggcg gtgtcggcgt cgccgtggcc gagcaccagg
     1801 cgcaggtaga gcgcggcgat ggtccaggtc ttgccggttc cggcgctggc ctcgatgagg
     1861 cggctgccgc gcagcggaaa gcaggcgggg tcgaggcggt ggctcatcgg gcagactcct
     1921 gggtctcggg tgccgggtgg gggacggcct cgacgtggct gtccatccat tcgagcagcg
     1981 ggccgtagag cgcgcgcgcg aggtgctcga agcggccgtc ctcgatcagc gcggcgtagt
     2041 cgggatagca gcgcgcgagg ctggcctcct cgacctcgcc gcgccggtgc tcgctgccct
     2101 cgtaggcttc ggccggcttg ccctcgccga cgagggcgag cgcggtgcgg gccgccaccg
     2161 gcagcggctc gcccaggccc gcgatccagg cctcgatcag gcgggcgagc agggcgcgcg
     2221 cgacggcggg gtcggtgggg acgatgtgga cggtggcgtc gcggccgacc agcaggccgc
     2281 cgctcgggct gtccgccgcg gcggcgagca ggctgcgcac ccagggcagc agcaggcgcc
     2341 aggggcgcag cgtgcccttg tcggcgtctt cgagcaggcg gccagggtcg tgggcgagcc
     2401 acgagggtag cgcgtgctcg tcggcgcgca cgcggtggtc cagccagtcc tccatgagcg
     2461 cgccggcatg cgcgaagcgc aggttgcggc gctcggcgtc gaggggatgg ctggcgcgga
     2521 ccagccgcca cgcgcgcagc agcggcagca cggcctcgcc gagtgcggcg cgggcgcgca
     2581 ggccgaggcc gccgatcggc agtcggccgg cgcgctcgat gcgtccgagc tgggcctcga
     2641 ccgcggcttc gagtgcgaag gcgtgcagtt cgggtggtgg cagcagggcg agttcggcga
     2701 gcaccgcggc ggcgagttcc tcgaccaggc cgtgttcctc gagaccgtcg aagccgaagg
     2761 cctcgtcgtc gggcagggcc tcgtccacgt cctcgtacac cacgccgagg cgctcgcgga
     2821 agaagacctt gaccgggttg cgcaggaagg cggcgagcgc cgcgatggtg agcgggaccg
     2881 ccgggtcggg gacgaagggg ggcagcggcg gggaggcagc ctcgccgcgc gtgggacgag
     2941 ttgcgcggcc cgtggcttcg cggttcgcct gcccgtcatg cgacggcacg gcatcgtcct
     3001 gcgctgccgc ctcgacgtgc gcggcgcgcc actcgcgcgc gtaggtgagc aagggcgtgc
     3061 ccggctcgaa gtagcgccgg ctgaagggct gcagcgggtg ctcgacggtg cgttcggcca
     3121 ccaccgcggg tgaccagccc gcggcgaggt agtcgcgcag ctgggcgacc agcaccgagg
     3181 gcggctgcac gctgttgtcg cgcacgttgc gcccgcacca gctcaggtag agcacccggc
     3241 gggccgagag cagggcctcg agcatgagct ggcggtcgtc gtcgcggcgg gcgcggtcgc
     3301 ccgggcgctg ctggcccggc agggccatga ggtcgaagtc gctgcgcggg ctgcggcgcg
     3361 gatagtcgcc gtcgttcatg cccagcaggc agaccacctc gaaggggatc gcacgcatcg
     3421 gcatcagcgt gcagaaggtg accccgccgg cgcggaagcg cttgtcgagc gcggggctct
     3481 cgagtgcggc gagccaggcc tcggcggcga cggcgagcgg cagctcggcc tcgaaagcgg
     3541 cctcgtcgca ggcctcgagc cagcgttcga gggcgtcgtc gagcgcggcc agggtctggc
     3601 ggtcggtgtc gtccaccgca cgcaccaggt cggcgagcag ggcgcgcagg cgctgggtcc
     3661 aggccgcggg cgtggcgggt cggcggctgt cggcctgcca gcggatcagg cgctcgacca
     3721 ggtgggcgag ggcgccggcg agctcggcgt cgaggccgcc gacctcgtcg tagggctcga
     3781 tgccggcgaa aggcgcgtcc atcgtgacct ccgaacgccc ggcggcgtag cccatcaaca
     3841 tgcggcgcag gccgaagagc gcgctgttct gctcgccgca ggcctgcagg ccgagccctg
     3901 cgcggtgacg cgcgtcgagg ccccagcgga tgccggcgcc gcgcatccac ccggtgagcc
     3961 gtggcagccc ctcctcggca aggccgaagc gggcggcgat ggcgggcacg tcgagcaggg
     4021 cgccgaggtc ggacagccgg cagcggtcgt gccggatgcg cagcagccac tccaccgcgc
     4081 ccatcagcgg gctgctcgcg cgcgccgaca ggtcggcgat gtcgaagggg atgtggcgcg
     4141 ggtcgtggcg gccgtactgg ccgaagaccg cgcggatcgc cggcgcgaag tcgtcgatcg
     4201 agggcaccat cacgacgatg tcgcgcgggc gcaggggttt gcctgcgtcc tggcgggcga
     4261 gcagggcgag gagctggtcg tggaggatct cgagctcgcg caccgggctg tgggcgaggt
     4321 ggaagacgat cgagcggtcc tcggccggta ccggcgggtg cggatgctcg gcgagcgggg
     4381 tgaggtcgcg gatgtggcgc tggacctgga cgagcagcgc gggtgcggcg ccgtcgtgcg
     4441 cgtcttcgcc gtcgtcgaag aggtcgatgc gggcgagcgg gaagcgcgcg cgcgtctgct
     4501 cggcgtcgtc gaaggcgtcg agctggcgca cgaagtcgcg cgcctggcga ccccaggcgg
     4561 cgaggagcgg gtgggcgtgc aggtgcatcg cgtccagccc gacgtcctgc agctcgcggc
     4621 cgtcgcgcag cggctggcgg cggcgggcga tgcgcagcca ctcgcgcccg tccaggatgt
     4681 cggcccagtg gaagcggcag gggttgggga tggcaagcag cacctgggcg tggcgcgaca
     4741 gcgcggcgag cagctgcagc gtgggcagcg gcacgtgggt catgccgaag agcacgacgc
     4801 ggcgcggcag ggcggcggcc ggacgcaggg cggtgtcctc ggccgtcgcc tccagcgtgg
     4861 cgagcacgcg atcgtgcagt tgcggccggg tgagtgcgcg ctgctgcggc tcgagctcgt
     4921 cgagcacgcg ctgccacagt tgcgcctgcc agcgctggtc ctcgggcagc ggggtagcga
     4981 tgcggcccgc gtcgagcagc tcttcgcgcc cctcgcccca ggtgtcgagc cagtcgggcc
     5041 ggtgcacctg atactggtcg tagaggtcgg cgatgcgcag cgcgagctgg aagcggcggc
     5101 cggggtcgtt atccgcgagg tagccggcga ccggcgcgaa gccgggttcg gccagcacct
     5161 cgggcagcag ccgcatcagc cgccaggcga gcgcggtctt gtcgagcggc gagcgcgccg
     5221 ggacctgcgc gcgcccgagc acctggcggt aggtctgcca ctggaagcgc gccggcagct
     5281 gcaccgccgc cgccgcgcat accccggccc ggcgcgcgat cgccatcttc acccattcgg
     5341 ccatgccgtt gctctgcacc aggatcacct cggattcgag gggggcgagc ggatgctgcg
     5401 ccagccagcc ggcgacggtg tcgagcaggg tctcgatgcg gttgccgtgc agggcgatga
     5461 agccgggggg gagggaggcg gcgggggcag gggcggtcat cttggagggc atcgttcagt
     5521 gcgcagccgt gcgggatggt ttggcgtatt cgtccgcatg cgccgcgagt cgtgcgcaga
     5581 ttatgcctcg tcccaccgga ccggctgggg gcggcgcagt ccggtgcatc gcgactcgcg
     5641 tcgctcctgc ggagagtcgt ggcgtgacgg caagagcctt gatcgcgact tgcgtcgctc
     5701 ctgcggagaa ctgctgcgac tgcggaggtc gttgcaggcg cgacgcgcgt cgcgatcaca
     5761 tgcgccgcgc gtacgcttcg cctc
//
LOCUS       DAOQNN010000192         5781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8067_length_5781_cov_9.485330, whole genome shotgun sequence.
ACCESSION   DAOQNN010000192 DAOQNN010000000
VERSION     DAOQNN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8067_length_5781_cov_9.485330"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(397..1092)
                     /locus_tag="PKV97_10610"
     CDS             complement(397..1092)
                     /locus_tag="PKV97_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HNB06370.1"
                     /translation="MSLHSSPGLVFDTPPIELETGPDPRYSVIWMHGLGADGSDFEPV
                     VPALALPRSPAVRFLFPHAPYRAVTCNAGYVMRAWYDIVSLAPHSRQIDEAGLLESRT
                     LIRQLIQREAERGVPAERVILAGFSQGGAVAYLTGLTHPTPLAGIIALSTYIPSPGLL
                     VDGFVEANRQVPVFAAHGTHDDVVSPALGEHAVEVLRQLGIEPEWHRYPLPHSVSLEE
                     IADIGRWLRTLVH"
     gene            1191..1436
                     /locus_tag="PKV97_10615"
     CDS             1191..1436
                     /locus_tag="PKV97_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HNB06371.1"
                     /translation="MNPEHLVRLLEREMPFGKYKGRVIADLPGYYLAWFARKGFPPGQ
                     LGQLLALMHELDHNDLAGLLEPLRRARRQAAAGPGGV"
     gene            1554..2678
                     /locus_tag="PKV97_10620"
     CDS             1554..2678
                     /locus_tag="PKV97_10620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HNB06372.1"
                     /translation="MPRFLHTADWQIGRQYARFAPEDALALAEARFSAVERLAGIARQ
                     EAVDAVLVAGDVFDAQTVSDRTIRRLFNAMGAYAGPWVLISGNHDAALAESVWSRARR
                     LGAVAPNVHLALGAQVMAFPERGFVVLPAPLTQRQTPADETAWFDHAETAPGLVRVGL
                     AHGSVQGVLAEDIDSPNPIAADRASRAALDYLALGDWHGTRRIDARTWYSGTPEQDRF
                     KANDPGNVLLVDIPAVGAEPEVEVRAVGRHPWIALEEKLAVASDLEGTIARLEALPEL
                     AVVDLSLSGEIHLLGRQRLEAALSVAEARFRSLRADLSGLRLQPTEDDLAALHADGYV
                     GRVIAELREEQDGENAEVASEALAILAALLLEAQAQGAAA"
     gene            2675..5311
                     /locus_tag="PKV97_10625"
     CDS             2675..5311
                     /locus_tag="PKV97_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB06373.1"
                     /translation="MRLLRLKIEQLRRFRQPVEIRDLDPGINLFTGPNESGKSTLVRA
                     IRAAFFERYKSSSVEDLQPWGDSAAAPTVELEFESQGKRWKLVKSFLKRKRCDVSVDG
                     VGFSGEEAEDKLADLLGFEFPGRGASRPEHWGIPGLLWIEQGAGQEIHKPVEHAGSHL
                     KSVLGASLGEVTSTAGDELIARITRERARLLTATGRPTGDFAAALEAHERLAREHADL
                     QGRIRQYRQQVDRLGQLREERARDESERIWEDYRRQARAADLRLAEVVSWQREQERDQ
                     QALQACQAGMRTSLDLLGRFEAQRKDLHRRAADRQAAEQALAAQDARRPAVERALGDA
                     RKAFEAARATVRLAREHQQRKDLVRELAQVDGELAGLDQALARARELHERWLRQRALR
                     QSLQVDAKALKRLRKVEEELARLAIRQEAVATRLQFDLREAGALTLGGEPLSGRGERL
                     LLEPTEILVAQLGSLRILPGGDDIADLVRTRQGLADERASLLSALGADSLAAADARHE
                     QCRELDESIKRDASVLESLAPEGLEALAGRKQLTQARREDLGKRIGALPEPQQDSLSV
                     SRAEAQLDAAEVSLKAAEADEAGFRSALLLATQALESARAEWSRLDGELQGADRKARE
                     KQAEDQLLELRAGENRLDQALRERQKQIDQANPGVLRQDVERFTRSADAAEKAAEQRR
                     LELERLRASLEALGAEGLEERSAELDVELARAARRSDELTRRARALDLLLVRLTDHRL
                     QLTRQLQAPLQRHLNRYLQLLFPEASLSVDEALVPEILVRQGHGGEERGRFAELSFGA
                     REQMGLISRLAYADLLREAGRPTLVILDDALVHSDVERLGNMKRVLFDAADRHQILLF
                     SCHPDSWRDLGVVPRDVAALGG"
     gene            5477..5599
                     /locus_tag="PKV97_10630"
     CDS             5477..5599
                     /locus_tag="PKV97_10630"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06374.1"
                     /translation="MHRTPWARAQVEALYLDTRPPAIGPSVGAQIHGPGAGIDS"
BASE COUNT          893 a         1928 c         2159 g          801 t
ORIGIN      
        1 cggcatgtcg ccgatctctt ccaggaacag cgtgccgcgc cagcaccatt tcaggtacag
       61 ggtccggcat gttgcggcgc accttgttgg tggcatccgg gcgtgagacg gcgccggccg
      121 ccatcacgga gtagccaaag acacggccga tccgatccgc aagcacgaaa gggcgacggg
      181 gccactcggc tgatttgcca gcgcgcgtcg cctgagccac tcgtcgagcg tggcttcccc
      241 gcaggcgagc ctgccgagca gatgggtggc tgcaagtggc tgcggcgcga tcgagggcga
      301 agtcctcgac gcacaagcgg tgcgcgcctc gattgcccgc cgtatgagtg tggacattgg
      361 cgccgtcgtg ccggcgggtc tcgccgggtc ttccggtcaa tgcacgaggg tgcgcagcca
      421 ccgaccgatg tcggcgatct cctcgaggct caccgagtgc ggcaaggggt agcggtgcca
      481 ttccggctcg atgcccagct gccggaggac ctcgaccgcg tgctctccga gtgcgggaga
      541 gacgacatcg tcgtgggtgc cgtgcgcggc gaagaccgga acctgtcgat tcgcttccac
      601 gaacccatcg accagcagcc cggggctcgg gatgtaggtc gacagcgcga tgatgccggc
      661 cagcggggtg gggtgggtga gcccggtcag ataggccacg gcgcccccct gcgagaaacc
      721 ggcgaggatg acgcgctcgg cgggcacgcc gcgctcggcc tcgcgctgga tcagttggcg
      781 gatgagggtg cgcgattcga gcagtccggc ctcgtcgatc tggcggctgt gcggggcgag
      841 cgagacgatg tcgtaccagg cgcgcatgac gtagccggcg ttgcaggtga ccgcacgata
      901 gggggcgtgc ggaaagagga agcgcacggc ggggctgcgg ggcagagcca gcgccgggac
      961 gaccggctcg aaatcgctgc cgtccgcgcc caggccgtgc atccagatga cgctgtagcg
     1021 gggatcgggt ccggtctcga gctcgatggg cggggtgtcg aagacgaggc ctggggagga
     1081 atggaggctc aagcgggtca ccttgtggga gagtggagcg tcggcgcgcg cggccgcgct
     1141 cgcaattggg cacggaaatg cggatgggca cggaaaaacc gatcgaacgg atgaatcctg
     1201 aacacctggt gcggctgctc gagcgcgaga tgcccttcgg caagtacaag gggcgggtga
     1261 tcgcggatct gccgggatac tacctcgcgt ggttcgcccg caagggtttc ccgccagggc
     1321 agctgggcca gttgctcgcg ctaatgcacg agctcgacca caacgacctg gccgggctgc
     1381 tcgagccgct gcgcagggcg cggaggcagg ctgccgcagg gccgggcggc gtgtaacatg
     1441 ccggccggtg acaaacaggg atgtgcatcg gtcatgcgcc cgggtccggt gatcggagcc
     1501 ccgggacggc ggctgtgcat gcacgacttt ccgctggatc aggggacgga ccgatgccgc
     1561 gctttcttca taccgccgat tggcagatcg gccgccagta cgcgcgcttc gcgcccgagg
     1621 acgcgctcgc gctggccgag gcgcgcttct cggcggtgga gcgcctggcg ggcatcgcgc
     1681 ggcaggaggc ggtggatgcg gtgctggtgg ccggggatgt cttcgatgcg cagacggtgt
     1741 ccgatcgcac gatccgccgg ctgttcaacg ccatgggggc gtatgcaggc ccgtgggtgc
     1801 tgatctccgg caaccacgac gcggcgctgg ccgagagcgt gtggtcgcgc gcccgccgcc
     1861 tcggggccgt ggcgcccaat gtgcatctgg cgctgggcgc gcaggtgatg gcgttccccg
     1921 aacgcggttt cgtcgtgctg ccggcgccgc tcacccagcg ccagaccccg gccgacgaga
     1981 ccgcgtggtt cgatcacgcg gagacggcgc ccggcttggt ccgcgtcggc ctcgcccatg
     2041 gcagcgtcca gggcgtgctc gccgaggata tcgactcgcc caacccgatc gccgccgacc
     2101 gcgccagccg cgccgcgctg gattacctcg cgctcggcga ctggcacggc acgcggcgga
     2161 tcgatgcgcg cacctggtat agcggcacgc ccgagcagga ccgattcaag gccaacgatc
     2221 cgggcaatgt gctgctcgtg gacatccccg cggtcggggc cgagccggaa gtggaggtcc
     2281 gggcggtggg ccggcacccc tggatcgcgc tcgaggaaaa gctggcggtg gcctcggatc
     2341 tcgagggcac gatcgcccgc ctggaggcct tgcccgagct cgccgtggtc gacctgagcc
     2401 tgtccggcga gatccacctg ctcggcaggc agcggctcga agccgcgctc tcggtggcgg
     2461 aggcgaggtt ccgcagcctg cgcgccgacc tctccgggct gcggctgcag cccaccgagg
     2521 atgatctcgc cgccctgcac gcagacggct acgtgggccg ggtgatcgcc gagctgcgcg
     2581 aggagcagga tggcgagaac gcggaggtcg cgagcgaggc gctggcgatc ctcgccgcgc
     2641 tgctgctcga ggcgcaggcc cagggagctg cggcatgagg ctgctgcgcc tgaagatcga
     2701 acagctgcgc cgcttccggc agcccgtaga gatccgcgat ctcgatcccg gcatcaacct
     2761 cttcaccgga ccgaacgagt ccggcaagag caccctggtg cgcgcgatcc gcgctgcatt
     2821 cttcgagcgc tacaagtcct ccagcgtgga ggatctgcag ccctggggcg attcggccgc
     2881 cgcgcccacg gtcgagctgg agttcgagtc gcagggcaag cgctggaagc tggtcaagag
     2941 cttcctgaag cgcaagcgct gcgatgtcag cgtagacggc gtggggttca gcggcgagga
     3001 ggcggaggac aagctcgcgg acctgctcgg cttcgagttc cccgggcgcg gggccagccg
     3061 accggagcac tggggcatcc ccggcctgct gtggatcgag cagggcgccg ggcaggagat
     3121 ccacaagccg gtcgagcatg cgggcagcca cctgaagtcc gtcctgggcg ccagcctcgg
     3181 cgaggtgacc agcaccgcgg gggacgagct gatcgcccgg atcacccggg agcgcgcgcg
     3241 cctgctgacc gccaccggcc ggcccaccgg cgacttcgct gcggcgctcg aggcgcacga
     3301 gcggctcgcg cgcgagcacg ccgatctgca ggggcggatc cggcagtatc gccagcaggt
     3361 ggatcggctc gggcagctgc gcgaggagcg ggcccgcgac gagtccgagc gcatctggga
     3421 ggactaccgg cggcaggcca gagcggccga cctccggctg gccgaggtcg tctcctggca
     3481 gcgcgagcag gagcgcgacc agcaggcgct gcaggcctgc caggccggca tgcgcacgag
     3541 cctcgatctg ctcggccgct tcgaggcgca gcgcaaggac ctgcacaggc gcgccgccga
     3601 ccgccaggcg gccgaacagg cgctcgccgc gcaggatgcg cgcaggccgg ccgtggagcg
     3661 tgcgctcggc gatgcccgca aggccttcga agcagcccgg gcgacggtca ggctggctcg
     3721 cgagcatcag caacgcaagg acctggtacg ggagctggcg caggtcgatg gcgagctcgc
     3781 cggcctggat caggcgctgg cgcgcgcccg cgagctgcac gagcggtggc tgcggcagcg
     3841 cgcgttgcgc cagtccctgc aggtcgacgc caaggcgctc aagcgcctgc gcaaggtgga
     3901 ggaggagctc gcccggcttg ccatccgcca ggaggcggtc gccacccgac tgcaattcga
     3961 cctgcgcgag gcgggcgcgc tcaccttggg cggcgagccc ttgagcgggc ggggggagcg
     4021 cctcctgctg gagccgaccg agatcctcgt cgcgcagctg ggaagcctgc gcatcctgcc
     4081 cggcggcgac gacatcgccg atctggtgcg cacgcgccag gggctggcgg acgagagggc
     4141 gagcctcctg tccgcgctcg gcgcggactc gctggccgcg gcggatgccc gccacgagca
     4201 atgccgcgag ctcgacgagt ccatcaagcg cgacgcatcg gtgctcgagt cgctggcgcc
     4261 cgagggcctc gaggcgctgg cgggtcgaaa gcagctcacg caggcgcgcc gggaggatct
     4321 cggcaaacgg atcggggccc ttcccgaacc gcagcaagac agcctctcgg tgtccagggc
     4381 cgaggcgcag ctcgacgctg ccgaggtgtc cctcaaggcg gccgaggccg acgaggcggg
     4441 cttccgcagt gcgctcctgc tcgccacgca ggcgctggag agtgcccggg ccgagtggag
     4501 ccggctcgac ggcgagctgc agggcgcgga tcgcaaggcg cgcgaaaagc aggccgagga
     4561 ccagctgctg gagctgcgtg ccggcgagaa ccggctcgac caggccctgc gagaacggca
     4621 gaagcagatc gatcaggcca atcccggcgt cctgcgccag gacgtcgaac gcttcacgcg
     4681 cagtgcggat gcggcggaga aggccgccga gcagcgccgc ctcgaactcg agcgcctccg
     4741 ggccagcctg gaggcgctcg gggcggaggg cctggaggag cgcagcgcgg aactggacgt
     4801 cgagctggcg cgtgctgcgc gccgtagcga cgagctcacg cggcgtgccc gcgcgctgga
     4861 cctcctgctc gtccgtctga cggatcaccg cctgcagctc acccgtcagc tgcaggcgcc
     4921 cttgcagcgc cacctgaacc gctatctgca actgctgttt ccggaggcca gcctgagcgt
     4981 ggacgaggcg ctcgtgccgg agatcctggt gcgccagggc cacggcgggg aagagcgtgg
     5041 ccgattcgcc gagctgagct tcggcgcgcg cgagcagatg ggcctgatca gtcgcctcgc
     5101 ctacgccgac ctgttgcgcg aggccggccg gccgacgctc gtgatcctgg acgatgcgct
     5161 ggtgcacagc gacgtcgagc gcctcggcaa catgaagcgg gtgctgttcg atgctgccga
     5221 ccggcatcag atcctgctgt tcagttgcca cccggacagc tggcgggacc tgggcgtcgt
     5281 gccccgggac gtcgccgcgc tgggaggctg agcgaccacg ccatgacccg ccgacggccc
     5341 gacaactcct tccacggtct cacgctggag gaggtcctct cccgacgcgc agaccggctc
     5401 ggcttgccga cgctcggcgg cgggtgagca tccgcggctt cgatcacgaa cccggcatcg
     5461 cctccggccc gagttcctgc accgcacgcc gtgggcgcgc gcacaggtcg aggccctgta
     5521 cctcgacacc cggccgcccg ccatcggccc gagcgtgggc gcgcagatcc acggtccggg
     5581 cgccggaatc gactcataag aatcttcgat tcattttgtg ttgacggatt ttctcgggtc
     5641 tctataatgc gcggcttccc gaacgcaaca gcgaatcggc gaaacagaaa agcagctgaa
     5701 ttctttcctt gccgcaagac ggcagatctt taaaaacttg gacaaccgat aagtgtgggt
     5761 gcttggttgt tgtggtcggc g
//
LOCUS       DAOQNN010000193         5780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8068_length_5780_cov_8.836681, whole genome shotgun sequence.
ACCESSION   DAOQNN010000193 DAOQNN010000000
VERSION     DAOQNN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8068_length_5780_cov_8.836681"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKV97_10635"
     CDS             <1..490
                     /locus_tag="PKV97_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNB06375.1"
                     /translation="TPKVAATAERIADANELPAAVARYLEARGLPAAIALQPAAVLQA
                     LDWSGFELHERVAPDETLAVGCARWGIAETGSLVFHSDAETPILANFLPLHHLVMVRA
                     STVLAYLDDYAKATAGQRPPRNVNIITGASGTTDIEGSLVLGAHGPRYLHVVVVEDLP
                     AA"
     gene            complement(915..1562)
                     /gene="pdxH"
                     /locus_tag="PKV97_10640"
     CDS             complement(915..1562)
                     /gene="pdxH"
                     /locus_tag="PKV97_10640"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNB06376.1"
                     /translation="MSKHTPLSDLRKSYEQGTLEEHDSASEPSRQFEAWLSQALERGL
                     PEPNAMTVATVGEDGRPSTRPVLIKGCDARGIVWYSNYHSRKGRELEVHPFAALQFHW
                     VEMERVVRIEGRVEKVSAEESDAYFASRPLAHRIGAWASPQSEVIDGRTTIVSRAAEY
                     GLKFGLNPPRPPHWGGYRLVPDYWEFWQGRSSRLHDRIAYRLQADGSWTRQRLAP"
     gene            complement(1700..3118)
                     /locus_tag="PKV97_10645"
     CDS             complement(1700..3118)
                     /locus_tag="PKV97_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNB06377.1"
                     /translation="MSPSTSASPTGTRAIVGQLLHLAWPILIAQLLSMTMMVADTVIA
                     GRYGTADLAGVAIGSSYYISVVMLLTGTLQAISPTIAHHVGAGRQEAIAPALQQGFWL
                     ALLLTGPGIALLLNPGFLLEAASVPPAVATKAADYLAATAFGLPALLLYRSFYAFNNA
                     VGRPRVLMAISAITMSVHIPLAWALTNGALGFAPMGGTGCGVSTATVHWIALGCGFAY
                     LVFNPAYKPYRLFHAWQAPRAQALGQLLRLGLPMGVSTFIDISSFTLIAILAARLGTE
                     TVAGHRVIANFTGMIYMLPLSLSIATMVLVGQSAGAQDWRRARRTARLGMSLALGFSL
                     LVGALLWMLREPLVAFSSADPAVQAVALGLVVWLLLYQCFDALQTVAAHALRGYKVTL
                     LPMLVHSLCFWGIGLAGGYWLSFHAPWRASAPTVAGFWEACVLATLAATLLFGALLRA
                     VSRRHVREAAVQERRKARMRRR"
     gene            complement(3198..4436)
                     /locus_tag="PKV97_10650"
     CDS             complement(3198..4436)
                     /locus_tag="PKV97_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HNB06378.1"
                     /translation="MLAHLERPAGPAPGLRRILDDFGLTYFANGLIAFIFSATGPLAV
                     ILSVGTRGGLSQSELASWVFGVFFVNGLVTLTMSLRYKMPLGFAWTIPGTVLVGPALQ
                     HLSFAEVIGAFYATSLLVLAMGASGWVKRVMSALPMPIVMGMVAGVFLRFGLDIVRAL
                     DSDVAIAAPMIIAFLLLSASPAWAKRMPPVIGALLIGALLIGALGRVDTSTLGSLAFA
                     QPVVQAPVWSLGALVELVVPLAITVLVVQNGQGTAVLRGAQHEPPVNAIAVACGVGAF
                     FSAMVGAVSTCLTGPSNALMTSSGKMDRQYTAALTFGLFGVLFGLLAPTFTGLMLAAP
                     KEFIMVLGGLAMLRVLQAAFLGSFGGGKFTLGALVSLIVTVADIGMFNIGAAFWGLVA
                     GFGVSWLMEKHDFTALANRA"
     gene            4554..5456
                     /locus_tag="PKV97_10655"
     CDS             4554..5456
                     /locus_tag="PKV97_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB06379.1"
                     /translation="MDRLQAMRLFTRIVELGSFSRAAEQLGLPRASATQIIQQLEAHL
                     GVRLLARTTRQVTPTVDGAGYYRRCLRILADLDDAEAEFSQAAHHPQGRIRIDLPDSF
                     CRLLLIPALPDFYTRYPLVRIEISVGDRQIDLIREGVDCVLRIGELGDVPLHARRLGR
                     LRQVTCASAGYLAQHPLPQGLEDLEAHWMVDYVSASTAKSLPLEFEVEGRIELRSLRS
                     RLAVNNGGAYVAACAAGFGIVQVPDYHVREPLEQGRFVEILPAFPPPVLPVHALYPAD
                     RPLSARVRVFIDWLAERFGGAAAG"
     gene            complement(5531..>5780)
                     /locus_tag="PKV97_10660"
     CDS             complement(5531..>5780)
                     /locus_tag="PKV97_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNB06380.1"
                     /translation="VGIFYLTLLPQFVPAGVQVASFIFLLATIHVLMSLLWFALLIAL
                     ARRIAPVLRRPAVIAGLDRLTGLVFVGFGVRLAVAQPG"
BASE COUNT          910 a         2033 c         2077 g          760 t
ORIGIN      
        1 cacccccaag gtcgcggcca ccgccgagcg catcgccgac gcgaacgagt tgccggccgc
       61 ggtggcgcgc tacctcgagg cgcgcggcct gcccgcggcg atcgcgctgc agccggcggc
      121 ggtgctgcag gcgctcgact ggtcgggctt cgagttgcac gagcgcgtcg cgcccgacga
      181 gaccctggcg gtgggctgcg cgcgctgggg catcgccgag accggctcgc tggtcttcca
      241 ttcggatgcc gagacgccga tcctcgccaa cttcctgccc ctgcaccacc tggtgatggt
      301 gcgcgcaagc acggtgctcg cctacctcga cgactacgcg aaggccaccg ccggccagcg
      361 cccgccgcgc aacgtcaaca tcatcaccgg cgcgagcggc accaccgaca tcgagggcag
      421 cctggtgctc ggcgcgcacg ggccgcggta tctgcatgtg gtggtcgtgg aggatctgcc
      481 cgccgcttga gtgcgccgcg cccggattcc gcgccgcgcc ggctcatccg ccgtgatcgc
      541 gactcgcgtc gcgcctgcgg gaagcgacgc ggcttcggct ggccggtagg agcgacgcaa
      601 gtcgcgatac agcgcccgca ccccgcgccg cgctggctca tccgccgtga tcgcgactcg
      661 cgtcgctccc acgagaagcg acgcggcttc aactggcccg tgggagcgac gcaagtcgcg
      721 atacagcgcc cgcaccccgc gccgcgctgg ctcatccgcc gtgatcgcga ctcgcgtcgc
      781 gcctgcggga agcgactcgg cttcggctgg cccgtaggag cgacgcaagt cgcgatactg
      841 catccgatcc ccgcaccgtg cttgccaggc ccgatcgcgg ctcgcgccct tccatgacac
      901 gctccgatgt cctgctacgg tgccaggcgt tgtcgcgtcc agctgccgtc ggcctgcagg
      961 cggtaggcga tgcggtcgtg caggcgcgaa ctgcggccct gccagaactc ccagtagtcc
     1021 ggcacgaggc ggtagccgcc ccagtgcggc gggcgcggcg ggttgagacc gaacttgagg
     1081 ccgtactcgg cggcgcgcga gacgatcgtg gtgcgcccgt cgatgacctc gctctgcggc
     1141 gaggcccagg cgccgatgcg gtgggcgagc ggacggctgg cgaagtacgc gtcggactcc
     1201 tccgccgaca ccttctcgac gcggccctcg atgcgcacca cgcgctccat ctccacccag
     1261 tggaactgca gcgcggcgaa ggggtgtacc tcgagctcgc gccctttgcg gctgtgatag
     1321 ttcgagtacc acacgatgcc gcgcgcgtcg cagcccttga tcagcaccgg ccgggtcgac
     1381 ggccggccat cctcgccgac ggtggccacg gtcatcgcgt tgggttcggg cagcccgcgc
     1441 tcgagcgcct gcgacagcca ggcctcgaac tgcctcgagg gctcgctcgc cgagtcgtgc
     1501 tcttccagcg tgccttgttc gtagctcttg cgcagatcgg acaggggggt gtgcttgctc
     1561 atcgttcttt ccaggggatg ccgggggaag tcaccaagct ccattgtcgc caaatcgtcc
     1621 cccctcgcgt caggattctc ccatgcagga gcggcgccag ccgcgaacac ggcggctcgg
     1681 cactcgacgc acgtcgcaat caccgccgcc gcattcgcgc cttgcggcgc tcctgcacgg
     1741 ccgcctcgcg cacgtggcga cgggagactg cgcgcagcag ggcgccgaag agcagggtgg
     1801 cggccagcgt cgccaggacg caggcctccc agaagccggc caccgtcggc gcgctggcgc
     1861 gccagggggc gtggaagctg agccagtagc cgcccgccag cccgatgccc cagaagcaca
     1921 ggctgtgcac cagcatcggc agcagcgtga ccttgtagcc gcgcagggcg tgggcggcga
     1981 cggtctgcag ggcgtcgaag cactggtaga gcagcagcca caccaccagc ccgagcgcga
     2041 ccgcctgcac cgccgggtcg gcggacgaga acgccaccag cggctcgcgc agcatccaca
     2101 gcagcgcacc caccagcagc gagaagccga gcgcgaggct catccccagc cgcgcggtgc
     2161 ggcgcgcgcg gcgccagtcc tgcgcgccgg ccgactgccc caccagcacc atggtggcga
     2221 tcgacagcga cagcggcagc atgtagatca tgccggtgaa gttggcgatg acgcggtgcc
     2281 cggccaccgt ctcggtgccg aggcgggcgg cgaggatggc gatcagggtg aaggagctga
     2341 tgtcgatgaa ggtcgacacc cccatcggca ggccgagtcg cagcagctgg ccgagcgcct
     2401 gcgcgcgcgg cgcctgccag gcgtggaaga ggcgataggg tttgtaggcc gggttgaaga
     2461 ccaggtaggc gaagccgcag ccgagcgcga tccagtgcac cgtcgcggtc gatacgccgc
     2521 agccggtgcc gcccatcggc gcgaagccga gcgcgccgtt ggtgagcgcc caggcgagcg
     2581 ggatgtggac agacatggtg atcgcgctga tcgccatcag cacgcgcggc cgccccaccg
     2641 cgttgttgaa ggcgtagaag ctgcggtaga gcagcagcgc gggcaggccg aaggcggtcg
     2701 cggcgaggta atcggcggcc ttggtggcga ccgcgggcgg cacgctcgcc gcctcgagca
     2761 ggaagcccgg gttgagcagc agcgcgatcc cggggccggt gagcagcagc gccagccaga
     2821 agccctgctg cagcgcgggg gcgatcgcct cctggcggcc ggcgccgacg tggtgtgcga
     2881 tcgtcggcga gatcgcctgc agcgtgccgg tgagcagcat caccaccgag atgtagtagc
     2941 tgctgccgat ggcgacgccg gcgaggtccg cggtgccgta gcgcccggcg atcacggtgt
     3001 ccgccaccat catcgtcatc gacagcagct gggcgatcag gatcggccac gccaggtgga
     3061 gcagctgccc gacgatggca cgggtgccgg tgggggaggc ggaggtggag gggctcatgg
     3121 aggggtggcg ccggcggacc cccggagagg ggccctccgc accggaatgc aggtgggggc
     3181 aggcctgccg cggcggctca ggcgcggttg gcgagcgcgg tgaagtcgtg cttttccatc
     3241 agccaggaca cgccgaagcc ggcgaccagg ccccagaagg cggcgccgat gttgaacatg
     3301 ccgatgtcgg cgacggtgac gatcaggctc accagcgcgc cgagcgtgaa cttgccgccg
     3361 ccgaacgagc ccaggaaggc ggcctgcagc acccgcagca tggccaggcc gccgagcacc
     3421 atgatgaact ccttgggagc ggccagcatc aggccggtga aggtcggcgc cagcaggccg
     3481 aacaggacgc cgaagagccc gaaggtgagc gcggcggtgt actggcggtc catcttgccc
     3541 gaggaggtca tcagcgcatt gctggggccg gtcaggcagg tgctgaccgc gccgaccatc
     3601 gcgctgaaga acgccccgac gccgcacgcg acggcaatcg cattgaccgg cggctcatgc
     3661 tgggcgcccc tcagcaccgc ggtcccctgg ccgttctgca ccaccagcac ggtgatcgcc
     3721 agcggcacga ccagctcgac cagcgccccc agcgaccata ccggggcctg caccaccggc
     3781 tgggcgaagg cgaggctgcc gagcgtcgag gtgtcgacac gaccgagcgc accgatcagc
     3841 agcgcgccga tcagcaaggc gccgatcacc ggcggcatgc gcttcgccca cgccggactc
     3901 gccgacagca gcaggaaggc gatgatcatc ggcgcggcga tcgccacgtc gctgtcgagc
     3961 gcgcgcacga tgtccagccc gaagcgcagg aacacccctg ccaccatgcc catcacgatc
     4021 ggcatcggca gcgccgacat cacccgcttg acccagccgc tggcgcccat tgccagcacc
     4081 agcaggctgg tggcgtagaa ggcgccgatc acctcggcga agctcaggtg ctgcagcgcg
     4141 gggccgacca gcacggtgcc gggaatcgtc cacgcgaagc cgagcggcat cttgtagcgc
     4201 aggctcatcg tgagcgtgac cagaccgttg acgaagaaca cgccgaacac ccaggaggcc
     4261 agctcggact ggctgaggcc gccgcgggtc cccacggaca ggatcacggc aagcggaccg
     4321 gtcgccgaaa agatgaaggc gatcaggccg ttggcgaaat aggtcaggcc gaagtcgtcg
     4381 aggatgcggc gcagccccgg ggcggggccg gcggggcgtt cgaggtgggc gagcatggcg
     4441 ttcgaggggg ctggggtcga gggtgtcggg ctgtggatga tcgctgtttg ctgtcgacag
     4501 ataaagacgc ataaaacaac tacactattt ggtttatacg aataatcgcc gccatggatc
     4561 gtctgcaggc catgcgcctc ttcacccgca tcgtcgagct cggcagcttc agccgcgccg
     4621 ccgagcagct cggcctgccg cgcgcgtcgg cgacgcagat catccagcag ctcgaggccc
     4681 acctcggcgt gcgcctgctc gcgcgcacca cgcggcaggt caccccgacc gtggacggcg
     4741 cgggctacta ccggcgctgc ctgcgcatcc tcgccgacct cgacgacgct gaggccgagt
     4801 tctcgcaggc cgcgcaccac ccgcaggggc gcatccgcat cgacctgccg gattccttct
     4861 gccggctgct gctgatcccg gccctgcccg acttttacac gcgctatccg ctggtccgca
     4921 tcgagatcag cgtcggcgac cgccagatcg acctgatccg cgagggcgtg gactgcgtgc
     4981 tgcgcatcgg cgagctcggc gacgtgccgc tgcacgcgcg ccggctcgga cgcctgcgcc
     5041 aggtgacgtg cgcgagcgcg ggctacctcg cgcagcatcc gctgccgcag ggcctcgagg
     5101 acctggaggc gcactggatg gtggattacg tctccgcctc caccgcgaag tcgctgccgc
     5161 tggagttcga ggtggagggc cgcatcgagc tgcgcagcct gcgctcgcgg ctggcggtga
     5221 acaacggcgg tgcctacgtg gccgcctgtg cggccggctt cggcatcgtc caggtgcccg
     5281 actaccacgt gcgcgagccg ctggagcagg gccgcttcgt cgagatcctg cccgcgttcc
     5341 cgccgcccgt gctgccggtg cacgcgctct acccggcgga ccggcccttg tccgcgcgtg
     5401 tgcgggtgtt catcgactgg ctggcggagc gtttcggcgg agcggcggca ggctgagccg
     5461 ggcgcgtccg ccctgtcgtg cgcgagtgcc cgttcgccgc gtccgcgtcc gcggaggacc
     5521 tgccgcccga tcagcccggc tgcgccaccg cgaggcgcac gccgaagccg acgaagacca
     5581 gcccggtgag ccgatccagg ccggcgatca ccgccggacg gcgcagcacc ggggcgatgc
     5641 ggcgcgcgag tgcgatcagg agggcgaacc agagcaggga catgagcacg tggatggtgg
     5701 cgagcaggaa gatgaagctc gccacctgca ctccggccgg cacgaactgt ggcagcaggg
     5761 tcaggtagaa gatgccgacc
//
LOCUS       DAOQNN010000194         5777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8078_length_5777_cov_13.104334, whole genome shotgun sequence.
ACCESSION   DAOQNN010000194 DAOQNN010000000
VERSION     DAOQNN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8078_length_5777_cov_13.104334"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2855)
                     /locus_tag="PKV97_10665"
     CDS             complement(<1..>2855)
                     /locus_tag="PKV97_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNB06381.1"
                     /translation="VTWTVTNRGTRATRSGSWYDGLYLSRDASLDNSDYALVDRGSPS
                     EAYIRLKAITVPASDLAAGKFLRPGESYTATATFNVPESISGDFRLIVKADTAIGKDG
                     SGYERSSIRDGLPGLIRFTDPAGGVLEFQDEGNNSRAIALTIDLATPPDLQVAHVTAP
                     ASILAGQTFTVNYRVVNQGGNTPSDQRTWNDLVYLSKDRFLDVNQDRYLGYLQHDGGL
                     AGGAGYDASLTVSAPKNLEGPYYVFVVSDPARAWGTGEYGKVREFGKEQNNATAAAQP
                     LLIETPPPADLKVTNIVLPASAQVGDELRLDYTITNDSINPAYGRWTDAVYLSADSGW
                     DLGDILLGKVDHIGDLAGGASYGGTLTAKLPPLKDGNWRIIVRPDLYNEVFEGKISYS
                     VTGLNLPPGEANNRMASGATLAVSVPTLTVATPLATTLSPGEARLYKLSVAAGETLRV
                     SLDAAADEGSNELYIRYGDIPTGYAFDAAYDNPVAADQEAMIPSTKAGDYYILVRAGQ
                     GNANAPVTLRADLLPLSITRVTPDQGGTGDDEHRWVTLDIYGSHFKAGALVKLSRPGV
                     AEAEPDRWQVLDATHIRAVFDTRKLPHGLYDVSVSNPDGQRVSEAQRYLVERGIEADV
                     TIGIGGARNLNPGESSVYSLSLQSLSNLDTPYVRFDFGVPEMGRSEDVLAGLNLPYLV
                     FGSNVGGQPDGLTVDSAGNTQTYGATPTDGTPRSDIPWARLDGVQNTAGFNLAPGYAF
                     DIPAGGFVGMSFNVQTYPGLAEWIAHDFEGLRAKLYAVRPDWAAQRLLDGGVQDLDKI
                     QEGLAAKFLWVDKDQDNTHISKLEALAMPFRFNVAGAATPLTRDEFIADQTAHAKRLR
                     GAILADTSAPANLSALAADEGQWVTGWLGALEAAGLLRPSNEAPPIRDNPQVLSLNAT
                     LATGILLSKGGDSYRTQADLLGFFAKVQQWYGDTARYG"
     assembly_gap    2856..2955
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2956..>4491)
                     /locus_tag="PKV97_10670"
     CDS             complement(<2956..>4491)
                     /locus_tag="PKV97_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNB06382.1"
                     /translation="NPVSLGSFTYDRGLESGIRYTRTEQVRLPAKIEGLYRVKVVTNA
                     NLGSGGNQVYEFGAARSNNTLVSADATQVSMYDRPDLRVATLAVPDHVTAGTSAAVRY
                     TINNMGAAAATGRWTDKVFLSLDATLSADDRLVGQSANGSALAPSEAYANESALVDIP
                     IRFRGDAYLIVVADGNNNVDEYPNESNNVKAAHFYIDPVPFGDLVTSNVLAPDQAVHG
                     SNIEVRYQVANLGSQTTRGESAALNAWTDTVWLARDKRRPGAHKGDILLGSFTHVGKL
                     AAGENYLGTVQVAIPENIRSGEYFVTVWSDTYDLILEDTLATSLNPDDPAQVDNNNYK
                     ARPISILGITPPDLVVKDVQAPASADAEGLYTFAYSVQNRGDFFSGGWTDSVYLTDNP
                     DFAAAREVWHLGDYGQTRSLGNGEIYSVTQSVQLATSAKGRYLIVRTDAGNRVGESNE
                     GNNTGAIGSVVSNRPADLQVTEIRTEPSNYSGEETTITWTVSNFGAAVWAGTRSWVDT
                     VYLS"
     assembly_gap    4492..4591
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4592..>5777)
                     /locus_tag="PKV97_10675"
     CDS             complement(<4592..>5777)
                     /locus_tag="PKV97_10675"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNB06383.1"
                     /translation="ASGSWRERIYVDSGSAPTEVASLFITDGLAAGASKTRSVNLTLP
                     TSLADGSYRWLVRTDIDGTVYERAGEANNQAVSATSLLVARIDLVVSEVSAPSLLRSG
                     DSLHASWKVSNPGGVATGSWVDRVYLGKGGVLTQMAEVSRSGPLASGAFYTASADFTI
                     PLDFSGEYQLVVVSDPASLLADGNRGNNQGAQAVRIDFSPYADLSVSGLSAPERVIDD
                     PAALDVRWTVSNQGTGAGRSTSWTDRVVLSTDDTYGNGDDRVVGEFRHDGALAAGESY
                     SRLERVLTPAASSGRFKLFIISDARGEVFENFSEANNLGRLAHNVDIMPVAYADLQVA
                     SVTAEGAAASGRPLRVSWEVVNNGIGSTSSAEWSDYVWLSRNPDGSGVVTTFGSAGHI
                     GQL"
BASE COUNT          897 a         1715 c         1920 g         1045 t
ORIGIN      
        1 ccgtagcggg ccgtatcgcc ataccattgc tggaccttgg cgaagaagcc gaggaggtcg
       61 gcctgggtac gatagctgtc gccgcccttg ctgagcagga tgcccgtagc cagcgtggca
      121 ttgaggctga gaacttgtgg gttgtcgcgg atcggcggcg cttcgttcga cggacgcagc
      181 aatcccgccg cttccagggc gccgagccaa ccggtgaccc attgtccctc gtcggccgcc
      241 agagccgaga gattggccgg agccgacgta tccgccagga tcgcgccgcg cagccgtttg
      301 gcgtgcgcgg tctggtcggc aatgaactcg tcccgggtca gcggcgtcgc ggcgcctgcc
      361 acgttgaagc ggaaaggcat ggccagggct tcgagcttgc tgatgtgggt attgtcctgg
      421 tccttgtcga cccagaggaa ttttgccgca aggccttcct ggatcttgtc cagatcctgc
      481 accccgccgt cgagcagtcg ctgcgccgcc cagtccgggc gaacggcata aagcttggcg
      541 cgcaaaccct cgaagtcgtg ggcgatccac tcggccaggc cgggataggt ctgcacattg
      601 aagctcatgc cgacgaagcc gccggcaggg atatcgaagg catagcccgg ggcgagattg
      661 aagcccgcgg tgttctgcac gccgtccagg cgggcccagg gaatgtcgga acggggcgtg
      721 ccgtcggtgg gcgtcgcacc gtaggtctgg gtattgcccg cggaatcgac agtcaggccg
      781 tccggctggc cgccgacgtt gctgccgaaa acgagatacg gcaggttgag ccctgccagt
      841 acgtcttcgc tgcgccccat ctccgggacg ccgaagtcga agcgcacgta gggcgtgtca
      901 aggttggaga ggctttgcag cgacaggcta tagacgctgc tttcgccggg attgaggttg
      961 cgcgcaccac cgataccgat ggtgacgtcg gcttcgatgc cgcgttcgac cagatagcgt
     1021 tgcgcttcgc tgacgcgctg gccgtccggg ttgctgacgc tcacgtcgta gaggccgtgc
     1081 ggcagcttgc gcgtgtcgaa aactgcccgg atgtgggtcg cgtccagcac ctgccagcgg
     1141 tctggctcgg cttcggcaac accggggcgg gagagcttga ccagcgcgcc ggccttgaaa
     1201 tgcgagccat aaatgtcgag ggtgacccag cggtgctcat cgtccccggt gcccccttga
     1261 tcaggagtca cccgggtgat ggacagaggc agcagatccg cccgcagggt gaccggagca
     1321 ttcgcattcc cctgacccgc acgcacgagg atgtagtaat cgcccgcctt ggtactgggg
     1381 atcattgctt cctggtcggc agccaccggg ttgtcatagg ctgcgtcgaa ggcatagccg
     1441 gtcgggatgt ctccataacg gatgtagagt tcgttgctgc cttcgtcggc cgccgcgtcc
     1501 aggctcacgc gcagcgtttc gccggcggcg acgctgagct tgtagaggcg cgcttcgccc
     1561 gggctgagcg tcgtcgccaa gggcgtggcg acggtcagcg tggggacgct gaccgcgagg
     1621 gtagcgccgg aggccatgcg gttgttggct tcgcccggcg gcaggttgag gccggtgacg
     1681 ctgtagctga tcttgccttc gaagacttcg ttgtagagat cgggccggac gatgatgcgc
     1741 cagttgccgt ccttcaaggg gggcagtttt gctgtcaagg tgccgccgta gctggcgcca
     1801 ccggcgagat cgccgatgtg gtccactttg ccgagcagga tgtcgcccag atcccacccg
     1861 ctgtcggccg acaggtagac ggcgtcggtc cagcgaccgt acgcggggtt gatcgagtcg
     1921 ttggtgatcg tgtagtcgag gcgaagctcg tcgcccacct gcgcactcgc cggcagtacg
     1981 atgttggtga ccttgagatc ggccggcggc ggggtttcga tcaggagggg ctgagctgcg
     2041 gcggtggcat tgttctgctc cttgccgaac tcgcgtactt tgccgtattc gccggttccc
     2101 caggcgcgcg ccggatcgct gacgacgaag acgtagtacg gaccttcgag gttcttcggc
     2161 gcgctcaccg tcaggctggc gtcgtagccg gccccgccag cgagcccgcc atcgtgctgg
     2221 agatagccca ggtagcggtc ctggttgacg tcgaggaaac ggtccttgga gaggtagacg
     2281 aggtcgttcc aggtccgttg gtcgctcggg gtattgcccc cctggttgac gacccgatag
     2341 ttgacggtga acgtctgccc ggcgaggatg ctggcgggcg cggtgacgtg cgccacctgg
     2401 aggtcgggcg gcgtcgcgag gtcgatggtc agggctattg cgcgactgtt gttgccttcg
     2461 tcctggaact cgagcacgcc gccggccggg tcggtaaagc ggatcaggcc gggcagtccg
     2521 tcgcggatgc tgctgcgctc gtagcccgac ccgtccttgc caatcgccgt gtcggccttg
     2581 acgatcaggc ggaaatcacc gctgatcgac tcgggcacgt tgaaggtcgc cgtggcggta
     2641 taggactcgc ccgggcggag aaatttgcct gccgcaaggt cgctggcggg gacagtgatc
     2701 gctttcagcc ggatgtacgc ttcgctcggc gaaccgcgat caaccagcgc gtagtcgctg
     2761 ttgtccaggg aagcgtcgcg cgagagatag agaccgtcgt accagcttcc gctgcgtgtc
     2821 gcccgcgtgc cgcggttggt caccgtccag gtcacnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnngctca gatagaccgt atcgacccaa ctgcgcgttc cggcccaaac
     3001 cgcggcgccg aaattgctca ccgtccaggt gatggtggtc tcctctccgg agtagttgct
     3061 tggctcggtg cggatttctg tgacttgcaa gtcggcgggt cggttgctga ccaccgaacc
     3121 gatggcgcca gtgttgttgc cttcgttcga ttcgccgacg cggtttcccg catcggtgcg
     3181 cacgatcagg tagcgaccct tggcactggt cgccaactgg accgactgtg tcaccgagta
     3241 gatctcaccg ttccccaggc tgcgcgtttg gccatagtcg cccaggtgcc agacctcgcg
     3301 ggcggcggcg aagtccgggt tgtccgtcag atagacggaa tcggtccagc cgccggaaaa
     3361 gaaatcaccg cgattttgca ccgaataggc gaaggtgtag aggccttcgg cgtcggcgct
     3421 ggccggggcc tgcacgtctt tcaccaccag gtcgggcggc gtgataccga ggatgctgat
     3481 cggccgggcc ttgtagttgt tgttgtccac ctgggccggg tcatcgggat tgaggctggt
     3541 cgccagcgtg tcttcgagaa tgaggtcgta ggtatcgctc cagacggtga cgaagtattc
     3601 gccggagcgg atgttctcgg gaatcgccac ctggacggtg ccgagatagt tctcgccagc
     3661 ggcgagtttg ccgacgtggg tgaaggagcc gagcaggatg tcgcccttgt gggcgcccgg
     3721 gcggcgcttg tcgcgcgcca gccagacggt gtcggtccag gcgttgagcg ccgcagattc
     3781 accccgggtg gtttggctgc caaggttcgc gacctgatag cggacctcga tgttcgagcc
     3841 atgcaccgct tggtccggag caagcacgtt gctggtgacg agatcgccaa agggaacggg
     3901 atcgatataa aaatgtgccg ccttgacgtt gttggattcg ttgggatatt cgtccacatt
     3961 attgttgcca tcggcgacga cgatgagata ggcgtcaccg cgaaaacgga tggggatatc
     4021 gaccaacgcc gactcattgg catacgcctc gctgggagcc agtgcgcttc cattggcaga
     4081 ctgacccacc agacgatcgt cggcactcag ggtggcgtcc agcgagagga agaccttgtc
     4141 ggtccaccgg ccggtggcgg cggccgcccc catgttgttg atggtatagc ggacggccgc
     4201 gctggtgccg gcagtcacgt gatcgggaac ggcaagggtg gcgacccgca gatccggccg
     4261 atcgtacatg ctgacctggg tggcatccgc cgagaccagg gtgttgttgc tgcgggccgc
     4321 accgaattcg tacacctggt taccaccgct gcccaagttg gcattggtga ccaccttgac
     4381 ccggtagagt ccctcgatct tcgcaggcag gcggacctgc tcggtacgcg tgtagcggat
     4441 tccggattcg aggccccggt cgtaggtaaa gctgccgagg gagacgggat tnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccagctggc cgatgtggcc agccgatccg
     4621 aaggtggtga cgacgccgct gccgtccggg ttgcgcgaga gccagacgta atccgaccac
     4681 tcggccgaac tggtgcttcc gatgccgttg ttgaccacct cccagctcac ccgcaggggc
     4741 cgcccactcg ccgccgcgcc ttcggcggtg accgacgcca cctgcagatc ggcgtaggcg
     4801 acgggcatga tgtccacgtt gtgcgccagc cgcccgaggt tgttggcttc ggaaaagttc
     4861 tcaaagactt cgccgcgcgc atcgctgatg atgaagagct tgaagcgacc cgagcttgcc
     4921 gccggggtca gtactctttc gagccggctg tacgactcac cggcggcgag cgcgccgtcg
     4981 tgacggaatt cgccgacgac gcggtcgtcg ccgttgccat aggtatcgtc ggtcgacagg
     5041 acgacgcggt cagtccagct cgtgctgcgc ccggcgccgg tgccctggtt gctcaccgtc
     5101 cagcggacgt cgagcgcggc tggatcgtcg atcacccgtt cgggcgcact cagcccgctg
     5161 accgacaagt cggcataggg tgaaaagtcg atgcgcacgg cctgcgcacc ctggttgttg
     5221 ccccggttgc catcggccag cagcgaagcc gggtcactga ccacgacgag ctggtactcg
     5281 cccgagaagt cgagcggaat cgtgaagtcg gccgaggcgg tgtagaaagc gccgctggcc
     5341 agcggaccgc tgcgcgaaac ttcggccatc tgcgtcagga cgccgccctt gccgaggtag
     5401 acccggtcga cccaggaccc ggtggcgaca ccccccggat tggacacctt ccagctcgca
     5461 tgcaagctgt cgcccgagcg caacagactc ggcgcgctga cctcgctgac gaccagatcg
     5521 atgcgcgcca ccaatagcga tgtcgctgaa acggcctggt tgttggcttc gccggctcgt
     5581 tcgtagacgg tgccgtcgat gtccgtcctg accagccaac ggtagctgcc gtcggccagc
     5641 gatgtcggca acgtcaggtt tacgctgcgc gtcttgctgg cgccggccgc caggccatcg
     5701 gtgatgaaca ggctggcgac ctcggtcggc gcgctcccgc tgtcgacgta gatgcgctca
     5761 cgccacgatc cgctcgc
//
LOCUS       DAOQNN010000195         5754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8133_length_5754_cov_7.971223, whole genome shotgun sequence.
ACCESSION   DAOQNN010000195 DAOQNN010000000
VERSION     DAOQNN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8133_length_5754_cov_7.971223"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2628)
                     /locus_tag="PKV97_10680"
     CDS             complement(<1..2628)
                     /locus_tag="PKV97_10680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HNB06384.1"
                     /translation="MLPAYAELHCLSNFSFQRGASHPEELVAQAAAFGYSAIALTDEC
                     SFAGVVRAHRALQALPEAGRPRLIVGCELRLADGPCLVLLAADRAGYGRLSRLLTQAR
                     RSADKGRYHLTRAMLDATRADEAAAEALPGCLALLIPPEEFPPPGAAGDAAIALRSEA
                     RWLAKRFPGRSWIAHTPRLDGRDPARLHWILRIAQATGVPAVAAGGALMHEAARRPLA
                     DVMSALRLHCSVAEAGLALAPNAELRLHERATLARRHPPELLAETVAVAARCRFDLDE
                     LRYEYPAELVPADETPTRWLRRLVEGGLAWRYGKAGKEHRAAAPATIAAAPPPAEDPA
                     PPTVRAQIEHELALIAELGYEPYFLTVHDIVRFARERGILCQGRGSAANSVVCWALGI
                     TEVDPQLGIMLVERFISRERDEPPDIDVDFEHERREEVIQYLYRKYGRERAALAATVI
                     RYRARSALRDVGRALGLDETQIERLTREHHWFDGRHILPERLAEAGLDPASPVTQRLV
                     ALTEELIGFPRHLSQHVGGFVIARGRLDELVPVENAAMSDRTVIQWDKDDLDAVGLMK
                     IDVLALGMLSALRRGLALVSAWRGEALTLATIPRERKEVYEMLSRADSVGVFQVESRA
                     QMTMLPRLRPQRFYDLVVEVAIVRPGPIQGGMVHPYLQARERAARGEDPLDGLREEIR
                     GVLARTLGVPIFQEQVMQLAVVAADFTGGEADQLRRAMGAWRRKGELERYRQKLLDGL
                     ANNGYDPDFAQRLCQQIEGFGSYGFPESHAASFALLVYASAWLKCFAPAAFLAALLNS
                     QPMGFYAPAQLIQDARRHGVEVRAADVGASAWDCMLEAADGAQPAVRLGLRMIRGLGR
                     EAATRIAAARGEHAFADTQD"
     gene            2824..3795
                     /locus_tag="PKV97_10685"
     CDS             2824..3795
                     /locus_tag="PKV97_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB06385.1"
                     /translation="MSFPLSRKVLFGALALAFAMAMPAYAQQKSVAITAIVEHPALDS
                     VRDGVKEALAAAGYEDGKNLKWQYQSAQGNTGTAAQIARKFVGDKADVIVAIATPSAQ
                     AVAAATKDIPLVFSAVTDPVVAKLVPSMQPSGTNVTGVSDALELGKQVELIKRVVPAA
                     KRVGIVYNPGEANSVVVVEQLRELLPKHGLSLVEAAAPRSVDVGSAARSLIGKADVFY
                     TSTDNNVVSAYEALVKVGMDAKIPLVAADTDSVARGAVAAYGMDYKALGVQTGEIVVR
                     ILKGEKPGAIASETSNKLSLQVNPAAAQKQGITLAEDLVKSAAKVVQ"
     gene            3894..4784
                     /locus_tag="PKV97_10690"
     CDS             3894..4784
                     /locus_tag="PKV97_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB06386.1"
                     /translation="MSLYTLLGALEIGLIFSLVALGVYISFRLLRFPDLTVDGSFPLG
                     GAVAATMIAAGHDPFTATIAATLAGAIAGLITGWLNVSLKIMDLLASILMMIALYSIN
                     LRIMGKPNVPLITEPTVFNLLQPEALADYVFRPLLLVFVVLAVKLALDGFFATQSGLA
                     IRATGSNARMARAQGVNTGMAILAGMALSNALVGLAGALFAQTQGGADISMGVGTIVI
                     GLAAVIVGESLLPSRKLYLATIAVIVGAIVYRFFIALALNSDFIGLQAQDLNLVTAVL
                     VTIALVVPRLRAHWSGKKGA"
     gene            4786..5580
                     /locus_tag="PKV97_10695"
     CDS             4786..5580
                     /locus_tag="PKV97_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB06387.1"
                     /translation="MLKATDLRLTFNPGTPIETQALRGMSLEIPTGQFVTVIGSNGAG
                     KSTFLNAVSGEQKVDSGTIEIDGIDMTRQPVWGRARHVARVFQDPLAGTCEDLTIEEN
                     MALAKMRGERRGFSFAVKPALREEFRAQLSTLGLGLENRLSDRIGLLSGGQRQAVSLL
                     MAALQPSRLLLLDEHTAALDPRTAAFVLELTVRIVAEHKLTTMMVTHSMRQALDVGQR
                     TVMLHQGRVVLDVSGKEREGLDVHHLLDMFEKVRGEKIDDDALLLG"
BASE COUNT          840 a         2016 c         2051 g          847 t
ORIGIN      
        1 gtcctgggtg tcggcgaagg cgtgttcgcc acgcgcggcg gcgatgcgcg tggcggcctc
       61 gcggccgagg ccgcggatca tgcgcaggcc gaggcgcacc gcgggctgcg cgccgtcggc
      121 ggcctccagc atgcagtccc acgcgctggc gccgacgtcc gccgcgcgca cctccacccc
      181 atggcggcgg gcgtcctgga tcagctgcgc gggcgcgtag aagcccatcg gctggctgtt
      241 cagcagcgcg gcgaggaagg ccgcgggcgc gaagcacttg agccaggccg aggcatagac
      301 cagcagcgcg aagctggcgg cgtgggattc gggaaagccg tagctgccga agccctcgat
      361 ctgctggcac aggcgctggg cgaagtcggg gtcgtagccg ttgttcgcca gcccgtcgag
      421 cagcttctgg cggtagcgct cgagctcgcc cttgcgccgc caggcaccca tcgcgcggcg
      481 cagctggtcg gcctcgccgc cggtaaagtc ggccgcgacc accgcgagct gcatcacctg
      541 ctcctggaag atcggcacgc ccagcgtgcg cgccagcaca ccgcggatct cttcgcgcag
      601 gccgtccaac ggatcctcgc cgcgcgccgc acgctcgcgc gcctgcaggt agggatggac
      661 catgccgccc tggatcgggc cggggcgcac gatcgcgact tccaccacca ggtcgtagaa
      721 gcgttgcggc ctcaggcgcg gcagcatggt catctgcgcg cgcgactcga cctggaacac
      781 gccgaccgaa tcggcgcgcg acagcatttc gtagacctcc tttcgttcgc gcgggatggt
      841 cgccagcgtc agcgcctcgc cgcgccatgc cgacaccagg gcgaggccgc gacgcagcgc
      901 cgacagcatg ccgagcgcga gcacgtcgat cttcatcagg ccgaccgcgt cgaggtcgtc
      961 cttgtcccac tggatcacgg tgcgatcgga catggcggcg ttctccaccg gcaccagctc
     1021 gtcgagccgg ccgcgcgcga tgacgaagcc gccgacgtgc tgggagaggt ggcgcggaaa
     1081 gccgatgagt tcctcggtga gtgcgaccag cctttgcgtg accgggctgg cggggtcgag
     1141 cccggcctcc gcgaggcgct cgggcaggat gtggcggccg tcgaaccagt ggtgctcgcg
     1201 ggtgaggcgc tcgatctgcg tctcgtcgag gccgagcgcg cggccgacgt cgcgcagcgc
     1261 gctgcgtgcg cggtagcgga tcacggtggc ggcgagcgcg gcgcgttcgc ggccgtactt
     1321 gcggtagagg tactggatga cctcctcgcg gcgctcgtgc tcgaagtcga cgtcgatgtc
     1381 gggcggctcg tcgcgctctc gcgagatgaa gcgctcgacc agcatgatgc ccagctgcgg
     1441 gtcgacctcg gtgatgccca gcgcccagca caccaccgag ttggccgccg agccgcggcc
     1501 ctggcacagg atgccgcgct cgcgggcgaa gcgcacgatg tcgtgcacgg tgaggaagta
     1561 gggctcgtag ccgagctcgg cgatgagcgc gagctcgtgc tcgatctgcg cgcgcaccgt
     1621 gggcggggcg gggtcttcgg ccggcggcgg tgcggccgcg atcgtggcag gcgcggctgc
     1681 gcggtgctcc ttgccggcct tgccgtagcg ccaggccagc ccgccctcga ccagccggcg
     1741 cagccagcgc gtcggggtct cgtccgcggg gacgagctcg gcggggtact cgtagcgcag
     1801 ctcgtcgagg tcgaagcggc agcgcgccgc caccgccacg gtctcggcga gcagctcggg
     1861 aggatggcgg cgggcgaggg tcgcgcgctc gtgcaggcgc agctcggcat tgggcgcgag
     1921 cgccaggccg gcctcggcca cgctgcagtg caggcgcagc gcgctcatca cgtcggcgag
     1981 cgggcgccgc gcggcctcgt gcatgagcgc gccgccggca gcgaccgcag ggacgccggt
     2041 ggcctgggcg atgcgcagga tccagtgcag gcgggcggga tcgcgtccgt ccaggcgcgg
     2101 ggtgtgggcg atccaggagc gtccggggaa gcgcttcgcc agccagcgcg cttcgctgcg
     2161 cagggcgatg gccgcgtcgc cggcggcacc cggcggcggg aattcctcgg gtgggatcag
     2221 cagcgccagg cagccgggca gggcttcggc ggcagcctcg tctgcgcggg tggcgtcgag
     2281 catcgcgcgc gtgaggtggt agcggccctt gtcggcgctc cgcctggcct gggtgagcag
     2341 gcgcgagagc cggccgtagc cggcgcgatc ggcggcgagc aggacgaggc agggcccgtc
     2401 ggcgaggcgc agctcgcagc cgacgatcag gcgcggccgg ccggcttcgg gcagggcctg
     2461 cagcgcgcgg tgggcgcgca ccacgccggc gaaggagcat tcgtcggtga gggcgatcgc
     2521 gctgtagccg aaggctgccg cctgcgcgac gagctcctcg gggtgcgagg cgccgcgctg
     2581 gaagctgaag ttgctcaggc agtggagttc ggcgtaggcg ggaagcatgt gtggactgta
     2641 taaaaataca gttattcagg caagtcgggc gagtgtcctc cacggcgatt gcgggtccac
     2701 cgcaatcgcg caatccggac ctgctgttaa actcgcgtcc tttgcaacac cgtactggcc
     2761 aagatgccgg cctggtgcct ttttcgaaga attcccggcc gcaaggctgc caaggagacc
     2821 tccatgtcct tccccctgtc ccgcaaggtc ctgttcggcg cgctggcgct cgcgttcgcg
     2881 atggcgatgc cggcgtacgc acagcagaag tcggtcgcca tcaccgcgat cgtcgagcat
     2941 cccgcgctcg attcggtgcg cgacggggtc aaggaggcgc tggccgcggc cggctacgag
     3001 gacggcaaga acctgaagtg gcagtatcag agcgcccagg gcaacaccgg caccgcggcg
     3061 cagatcgcac gcaagttcgt cggcgacaag gccgacgtga tcgtcgcgat cgccaccccc
     3121 tcggcgcagg ccgtcgcggc cgcgaccaag gacatcccgc tggtgttttc ggcggtgacc
     3181 gatccggtgg tggccaagct ggtgccctcg atgcagccct cgggcaccaa cgtcaccggg
     3241 gtgtcggatg ccctcgagct cggcaagcag gtcgagctga tcaagcgcgt cgtgcccgcc
     3301 gccaagcgtg tcggcatcgt ctacaacccg ggcgaggcca actcggtggt cgtggtcgag
     3361 cagctgcgcg agctgctgcc caagcacggc ctgagcctgg tcgaagccgc ggcgccgcgc
     3421 tcggtggacg tcggctcggc ggcgcgcagc ctgatcggca aggccgacgt gttctacacc
     3481 agcaccgaca acaacgtcgt gtcggcctac gaggccctgg tcaaggtggg catggacgcc
     3541 aagatcccgc tcgtggccgc cgacaccgac agcgtggcgc gcggtgcggt cgcggcctac
     3601 ggcatggact acaaggcgct cggcgtgcag accggcgaga tcgtggttcg catcctcaag
     3661 ggcgagaagc ccggtgcgat cgcctccgag accagcaaca agctgtcgct gcaggtgaac
     3721 ccggctgcgg cgcagaagca gggcatcacc ctggcggaag acctcgtcaa gtcggctgcc
     3781 aaggtcgtcc agtaagcggg tcgcccgcat ccccgcgcgg ccgtcgatgc ccggccgcgg
     3841 gggacgcgtc ccggcgcgcg ttccgctcga tcctcaccaa cggtaatccc ccaatgtcgc
     3901 tctacacctt gctcggggcg ctggagatcg gcctgatctt cagcctcgtc gccctcggcg
     3961 tgtacatctc cttccggctg ctgcgctttc ccgacctcac cgtggatggc agcttcccgc
     4021 tcggtggcgc cgtcgccgcg accatgatcg ccgccggcca cgaccccttc accgcgacca
     4081 tcgcggcgac gctcgccggc gcgatcgccg ggctgatcac cggctggctc aacgtcagcc
     4141 tcaagatcat ggacctgctc gccagcatcc tgatgatgat cgccctgtac tcgatcaacc
     4201 tgcgcatcat gggcaagccc aacgtgccgc tgatcaccga acccaccgtg ttcaacctgc
     4261 tgcagcccga ggcgctcgcc gactacgtgt tccggccctt gctgctcgtc ttcgtggtgc
     4321 tggcggtcaa gctcgcgctc gacggtttct tcgccaccca gagcggcctg gcgatccgcg
     4381 cgacgggctc gaacgcgcgc atggcgcgcg cgcagggcgt caacaccggc atggcgatcc
     4441 tggccggcat ggccctgtcg aacgccttgg tgggcctggc cggggcgctg ttcgcgcaga
     4501 cccagggcgg cgccgacatc tcgatgggcg tgggaacgat cgtcatcggt ctcgcggcgg
     4561 tgatcgtggg cgagagcctg ctgccctcgc gcaagctcta cctggcgacg atcgcggtga
     4621 tcgttggcgc catcgtgtat cgcttcttca tcgcgctcgc gctcaattcc gacttcatcg
     4681 gcctgcaggc gcaggacctc aacctcgtca ccgcggtgct ggtgacgatc gcgctggtgg
     4741 tgccgcgcct gcgcgcgcac tggtccggca agaaaggagc ctgacatgct gaaggcaacc
     4801 gacctgcgcc tcaccttcaa ccccggcacg ccgatcgaga cccaggccct gcgcggcatg
     4861 tcgctcgaga tcccgaccgg gcagttcgtc accgtgatcg gctccaacgg cgccggcaag
     4921 tcgaccttcc tgaatgccgt ctccggcgag cagaaggtcg acagcggcac gatcgagatc
     4981 gacggcatcg acatgacccg ccagccggtg tgggggcgtg cgcgccacgt cgcgcgggtg
     5041 ttccaggacc cgctcgccgg cacctgcgaa gacctcacca tcgaggagaa catggcgctc
     5101 gccaagatgc gcggtgagcg ccggggtttt tcgttcgcgg tcaagccggc gctgcgcgag
     5161 gagttccgcg cccagctctc cacgctgggc ctcggcctgg agaaccgcct gtccgaccgc
     5221 atcggcctgc tctcgggcgg ccagcgccag gcggtgagcc tgctgatggc ggcgctgcag
     5281 ccctcgcgcc tgctgctgct cgacgagcac accgccgcgc tcgacccgcg cacggcggcc
     5341 ttcgttctgg agctcaccgt gcgcatcgtc gccgagcaca agctcaccac gatgatggtc
     5401 acgcactcga tgcgccaggc gctcgacgtc ggccagcgca ccgtgatgct gcaccagggc
     5461 cgcgtcgtgc tcgacgtctc cggcaaggaa cgcgagggcc tcgacgtgca tcacctgctc
     5521 gacatgttcg agaaggtgcg cggcgagaag atcgacgacg acgcgctgct gctgggctga
     5581 ttccccgggt cgagtctcgg ggccaagagc ctggggcggg gcctcttccg cgcaccggct
     5641 ggccaagcga cccgcctgat ccccgaattc aaacgaaaca cgaccgaaag caacgatgac
     5701 ctccgcttcc cgccccgtcc gtacccccgt gcgtacccgc ttcgccccca gccc
//
LOCUS       DAOQNN010000196         5745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8150_length_5745_cov_8.468717, whole genome shotgun sequence.
ACCESSION   DAOQNN010000196 DAOQNN010000000
VERSION     DAOQNN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8150_length_5745_cov_8.468717"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /gene="dctP"
                     /locus_tag="PKV97_10700"
     CDS             complement(<1..902)
                     /gene="dctP"
                     /locus_tag="PKV97_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNB06388.1"
                     /translation="MERRSFLKKASVGLAAGAAAGVASTAAVAAPAVQTGLPEIKWRM
                     TSSFPKSIDTLYGSAELLANRLREITGGKFDIRIFPAGEIVPGLQALDAVQQGTVEMC
                     HSCSYYYVGKDKTFAFGTAVPFGMNARQMDAWIIGGGGQELLDEFYGNYNVYSFLGGN
                     TGVQMGGWYRKQINTLEDIKGLKIRIAGIAGEIMSRMGAIPQQIAGSDIYPSLEKGTI
                     DAAEWVAPYDDEKLGFYKVAPNYYSPGWWEPGPVVHFYVNKAEWDKLPKEYQAAFRAA
                     SREAHIQMTAMYDHKNPQALARLLA"
     gene            complement(1019..>1852)
                     /locus_tag="PKV97_10705"
     CDS             complement(1019..>1852)
                     /locus_tag="PKV97_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNB06389.1"
                     /translation="NSHGFLGGYATTRHGLSCSVIAGEGDAMQREYWYDSRRDPAELM
                     VAEEVGRRAGERALARLGAKKIRTCEVPVLFEAQLAVALVGNFVQAVSGGSLYRKSSF
                     LLDSLGKQVFSPVVSIAERPHLKKAFGSSPFDGDGVATRDREVVTDGVLNGYFLSAYS
                     ARKLGLQTTANAGGSHNLIVKAGEQDLAGLIRQMDRGLLVTELLGHGVNYVTGDYSRG
                     AAGFWVEKGRIKHAVEEITIAGNLRDMFRGIVAVGNDALPRGAKLCGSVLIERMKVAG
                     R"
     assembly_gap    1853..1952
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1953..2224)
                     /locus_tag="PKV97_10710"
     CDS             complement(<1953..2224)
                     /locus_tag="PKV97_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HNB06390.1"
                     /translation="MSSQGFSFSQAHLAEIATDILKYARKHGASSCETDVSEGFGQSV
                     AVRRGEVDTIEYNRDKGVGVTVYLGQQRGYASTSDFSKKALKATVDA"
     gene            2281..2874
                     /gene="yjgA"
                     /locus_tag="PKV97_10715"
     CDS             2281..2874
                     /gene="yjgA"
                     /locus_tag="PKV97_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HNB06391.1"
                     /translation="MQAMRHHHSDPADEDDFPEPPSKSSRKREMQALQDLGEQLVALS
                     PERLKKVPLPETLYEAIRAAQGFKMEARRRQLQYIGKLMRKIDPEPIQAQLDIFAGNS
                     AAEVAKMHRLERLREQLLEDEQTIGTIAETWPEADLQYLRTLRRNALKEREAAKPPKS
                     FREIFRVLRELQEARDAAAEAAETPSDEDAAEQGPNS"
     gene            2871..3461
                     /gene="mog"
                     /locus_tag="PKV97_10720"
     CDS             2871..3461
                     /gene="mog"
                     /locus_tag="PKV97_10720"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HNB06392.1"
                     /translation="MSEHIRIGLVSISDRASEGTYEDQGIPALKDWLGRALTSPWSAE
                     TRLIPDERALIERTLVELVDRAGCNLVLTTGGTGPAVRDVTPEATLAIGDKEMPGFGE
                     EMRRISLNFVPTAILSRQVAVIRGKCLIINLPGQPKSIRETLEGVRDADGSVRHVGIF
                     AAVPYCIDLVGGPYVETDDAMIKAFRPKHAMRPKQA"
     gene            3485..4735
                     /locus_tag="PKV97_10725"
     CDS             3485..4735
                     /locus_tag="PKV97_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB06393.1"
                     /translation="MTWDELADDGSVRRCSARWRSESGQAPPRRVVIADDTTKADAAW
                     RLACEGSALLWRGDYHNARQLLQAMARRADARARKPGRAVKPGKAPATPAEAFHLHRM
                     AQAQRARALGMLLIPFEAGVRIPLRRAPEVAEVVAEAVAGTADADLAAAPFVMSLREL
                     LGLVGAHEWRRKGVAIAALGERIHPWYGVYSPIRGEYLDLIARAPLPATTLAFDIGTG
                     TGVIAALLARRGVARVVATDVSPRALGCAQQNLARLGLLAGAAAEEGAPGTTSCKIPG
                     AAAPDLQVAAGRVQLLQADLFPPGRAPLVVCNPPWVPAKPSSAIEHAVYDPDSRMLRG
                     FLAGLAEHMEEGGEGWLILSDLAEHLGLRSRAELLGWIEAAGLRVLGREDIRPRHGKA
                     ADADDPLHAARAAETTSLWRLAPR"
     gene            complement(4775..5458)
                     /locus_tag="PKV97_10730"
     CDS             complement(4775..5458)
                     /locus_tag="PKV97_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNB06394.1"
                     /translation="MNLSLRTPSAGTIVAFKAIVFALCLLPAVNLALGWQANALGANP
                     IETITRASGEWTLRLLLITLAVTPLRRFSGLHWLLRLRRMLGLFAFAYALGHFAIYLW
                     LDQFFDWNAIALDILDRPFITVGFAAFVLLIPLAATSNSFAIRRLGGRRWQSLHRSVY
                     AIAIFAVVHFWWLVKADIGRPLLYASILAVLLGLRGWWRELERRRQLSVPPPAKHLEK
                     KVIRLVPRQ"
     gene            complement(5484..>5745)
                     /locus_tag="PKV97_10735"
     CDS             complement(5484..>5745)
                     /locus_tag="PKV97_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004378077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mononuclear molybdenum enzyme YedY"
                     /protein_id="HNB06395.1"
                     /translation="KYGFKSAKSIVTIRLTEKEPATAWNKFAPQEYGFYSNVNPEVRH
                     PRWSQATERRIGELRKRPTQMFNGYAEQVASLYQGMDLKKFY"
BASE COUNT          877 a         1958 c         1979 g          831 t
ORIGIN      
        1 gccagcaggc gggcgagcgc ctgcgggttc ttgtggtcgt acatcgcggt catctggatg
       61 tgggcttccc gcgacgcggc gcggaaggcg gcctggtatt ccttcggcag cttgtcccac
      121 tcggccttgt tcacatagaa gtgcacgacg ggacccggct cccaccagcc cggcgagtag
      181 tagttcggcg ccaccttgta gaagccgagc ttctcgtcgt catacggggc cacccattcg
      241 gcggcgtcga tcgtcccctt ctcgagcgag gggtagatgt ccgagccggc gatctgctgc
      301 gggatcgcgc ccatgcgcga catgatctcg ccggcgatgc cggcgatgcg gatcttcagg
      361 cccttgatgt cttcgagggt gttgatctgc ttgcggtacc agccgcccat ctgcacgccg
      421 gtgttgccgc cgaggaagga atacacgttg tagttgccgt agaactcgtc gagcagctcc
      481 tggccgccgc cgccgatgat ccaggcgtcc atctggcgcg cgttcatgcc gaagggcacg
      541 gcggtgccga aggcgaaggt cttgtccttg ccgacgtagt agtaggagca cgagtggcac
      601 atctcgaccg tgccctgctg caccgcgtcg agcgcctgca ggccggggac gatctcgccg
      661 gccgggaaga tgcggatgtc gaacttgccg ccggtgatct cgcgcaggcg gttggcgagc
      721 aactcggcgc tgccgtacag ggtgtcgatg ctcttgggga agctcgaggt catgcgccac
      781 ttgatctcgg gcaggccggt ctggacggcg ggggcggcga cggccgcggt ggaggcgacg
      841 cctgcggcgg caccggcggc aaggccgacg ctggccttct tgaggaatga acgacgttcc
      901 acgatttctc cttgtgttcg ggggtggatt ccagcgaatt cagcgaacga ttatagacgc
      961 cgctcgcgca acgggacgga aaggtaccgc gctggcggga tgaagtccgg acaccggctc
     1021 agcgcccggc gaccttcatg cgctcgatca gcaccgagcc gcacagcttg gcgccgcgcg
     1081 gcagggcgtc gttacccacc gcgacgatgc cgcggaacat gtcgcgcagg ttgccggcga
     1141 tggtgatctc ctcgacagcg tgcttgatgc ggcccttctc cacccagaag cctgccgcac
     1201 cgcgcgagta atcgcccgtg acgtagttca cgccgtggcc gagcagctcg gtcacgagca
     1261 ggccgcggtc catctgccgg atcagtccgg cgaggtcctg ctcgccggcc ttcacgatca
     1321 ggttgtgcga gccgccggcg ttggcggtgg tctgcaggcc gagcttgcgc gccgaatagg
     1381 cggagaggaa gtagccgttc agcaccccgt cggtgaccac ctcgcggtcg cgggtggcca
     1441 cgccgtcgcc gtcgaagggg ctggagccga aggccttctt caggtgcggg cgctcggcga
     1501 tgctcaccac cggcgagaac acctgcttgc cgaggctgtc gagcaggaag ctcgacttgc
     1561 ggtagagcga gccgccgctc accgcctgca cgaagttgcc gacgagcgcc accgcgagct
     1621 gcgcctcgaa cagcaccggc acctcgcagg tgcggatctt cttcgcaccc aggcgcgcca
     1681 gcgcgcgctc gccggcgcgc cggccaacct cctcggccac catcagctcg gcagggtcgc
     1741 gccgcgagtc gtaccagtat tcgcgctgca tggcgtcgcc ctcgccggcg atcaccgagc
     1801 acgacagacc gtggcgggtg gtggcgtagc cgccgaggaa gccgtggctg ttnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgtccac cgtggccttg agcgccttct
     1981 tcgagaagtc ggaggtgctc gcgtagccgc gctgctggcc gaggtatacc gtgacgccca
     2041 cccccttgtc gcggttgtac tcgatggtgt cgacctcgcc gcggcgcacc gcgaccgact
     2101 ggccgaagcc ctcggagacg tcggtctcgc acgacgaggc gccgtgcttg cgggcgtatt
     2161 tcaggatgtc ggtggcgatc tcggcgagat gcgcctgcga gaaggagaag ccttggctgg
     2221 acatgggcgg gccgggtgcg tgcaaaggct ataatcttac cccgatcgag ccagcccccc
     2281 atgcaagcaa tgcgccacca ccattccgac cccgcggacg aggacgattt tcccgagccg
     2341 ccgagcaaga gcagccgcaa gcgcgagatg caggcgctgc aggacctcgg cgagcagctc
     2401 gtcgcgctct cgcccgagcg cctcaagaag gtgccgctgc ccgagaccct gtacgaggcc
     2461 atccgcgccg cccagggttt caagatggag gcgcgccgcc gccagctgca gtacatcggc
     2521 aagctgatgc gcaagatcga ccccgagccc attcaggccc agctcgacat cttcgccggc
     2581 aactccgccg ccgaggtggc gaagatgcac cgcctcgagc gcctgcgcga gcagctgctc
     2641 gaagacgagc agaccatcgg caccatcgcc gagacctggc ccgaggccga cctgcaatac
     2701 ctgcgcaccc tgcgccgcaa cgcgctcaag gagcgcgagg ccgccaagcc gccgaagtcc
     2761 ttccgcgaga tcttccgcgt gctgcgcgag ctgcaggaag cccgcgacgc cgccgccgag
     2821 gcggcggaga ccccgtccga cgaggacgcc gcagaacagg gaccgaactc atgagcgaac
     2881 acatccgcat cggcctcgtg tcgataagcg accgcgcctc cgagggcacc tacgaagacc
     2941 agggcatccc cgcgctgaaa gactggctgg gcagggcgct cacgtcgccg tggagcgccg
     3001 agacccggct gatccccgac gagcgtgcgt tgatcgagcg caccctggtg gagctggtcg
     3061 accgggctgg ctgcaacctc gtgctcacca ccggcggcac cggtccggcg gtgcgcgacg
     3121 tgacccccga ggccacgctc gcgatcggcg acaaggagat gcccggcttc ggcgaggaga
     3181 tgcgccgcat cagcctgaac ttcgtaccca ccgcgatcct gtcgcgccag gtcgcggtga
     3241 tccgcggcaa gtgcctcatc atcaacctgc ccggccagcc caagtcgatc cgcgaaaccc
     3301 tcgaaggcgt gcgcgacgcc gacggcagcg tgcgccacgt cggcatcttc gccgcagtgc
     3361 cgtactgcat cgacctcgtc ggcggtccgt atgtcgagac cgacgacgcg atgatcaagg
     3421 ccttccgccc caagcacgcg atgcggccga agcaggcgtg agcccctcga gcgcagcggc
     3481 gacgctgacc tgggacgagc ttgcggacga cggcagcgtg cgccgctgca gtgcgcgctg
     3541 gcgttccgag agcggccagg cgccaccgcg ccgggtggtg atcgccgacg acaccaccaa
     3601 ggccgatgcg gcctggcgcc tggcctgcga gggcagcgcc ctgctgtggc gcggcgacta
     3661 ccacaacgcc cgccagctgc tgcaggcgat ggcgcgccga gccgacgcgc gcgcgcggaa
     3721 accgggcagg gcggtcaagc cgggcaaggc gccggccacg cccgccgagg ccttccacct
     3781 ccaccgcatg gcgcaggcgc agcgcgcgcg cgccctgggc atgctgctga tccccttcga
     3841 ggccggcgtg cgcattccgc tgcggcgcgc gcccgaggtc gccgaagtcg tcgccgaggc
     3901 ggtcgccggc acagcggatg cggacctcgc tgcggcaccc ttcgtgatgt cgctgcgcga
     3961 gctgctcggc ctcgtcggcg cgcacgagtg gcggcgcaag ggcgtcgcca tcgctgcgct
     4021 cggcgagcgc atccacccct ggtacggcgt gtattcgccg atccgcggcg aatacctgga
     4081 cctgatcgcg cgcgcgccgc tgcccgcgac cacgctcgcc ttcgacatcg gtaccggcac
     4141 cggcgtcatc gccgcgctgc tcgcgcgccg cggggtggcg cgcgtggtgg ccaccgacgt
     4201 gtcgccgcgt gcgctgggtt gcgcgcagca gaacctcgcg cgcctcgggt tgctggccgg
     4261 ggccgcggcg gaggagggcg ccccgggcac aacctcctgc aagatcccgg gcgccgctgc
     4321 gcccgacctt caggttgccg ccggccgcgt gcagttgctc caggccgatc tcttcccgcc
     4381 cggccgcgcg ccgctggtgg tgtgcaaccc gccctgggtg ccggccaagc cgagctcggc
     4441 gatcgagcac gcggtctacg accccgacag ccgcatgctg cgcggcttcc tcgccggcct
     4501 ggcggaacac atggaggaag gtggcgaggg ctggctgatc ctgtcggacc tcgccgaaca
     4561 cctcggcctg cgcagtcgcg ccgagcttct cggctggatc gaggccgcgg gactgcgcgt
     4621 cctcggacgc gaggacatcc gtccgcgcca cggcaaggcg gccgatgcgg acgatccgct
     4681 gcatgcggcg cgcgcggcgg aaacgacctc gctctggcgc ctggctccgc gctgaggcgt
     4741 ggcgccgccg ggcccgacgg cgccgcggtg ccgcctactg ccgcggcacc agccggatca
     4801 ccttcttctc caggtgcttc gccggcggcg gcaccgagag ctgccgccgg cgttcgagtt
     4861 cgcgccacca gccgcgcagg ccgagcagca cggcgaggat gctcgcgtag agcagcggac
     4921 gcccgatgtc cgccttcacc agccaccaga agtgcaccac ggcgaagatc gcgatcgcat
     4981 agacagagcg gtgcagcgac tgccagcggc gcccgccgag gcgtcggatc gcgaagctgt
     5041 tcgaggtcgc ggcgagcgga atgagcagca cgaaggcggc gaagccgacg gtgatgaagg
     5101 ggcggtcgag gatgtcgagg gcgatcgcgt tccagtcgaa gaactggtcc agccacagat
     5161 agatcgcgaa gtgcccgagc gcgtacgcga acgcgaagag gccgagcatg cggcgcaggc
     5221 gcagcagcca gtgcaggccg ctgaagcggc gcagcggcgt tacggcgagt gtgatcagca
     5281 gcaggcgcag cgtccattcc ccgctcgcgc gcgtgatcgt ctcgatcggg ttggcgccga
     5341 gcgcgttcgc ctgccagccg agcgcaaggt tcactgcggg cagcaggcag agggcgaaca
     5401 cgatcgcctt gaaggcgacg atcgtgcccg cggagggggt tctgagggaa agattcatcg
     5461 ttgcggtcgg cgggatgcag tgctcagtag aacttcttca ggtccatgcc ctggtacagg
     5521 ctcgcgacct gctccgcata gccgttgaac atctgcgtcg ggcgcttgcg caactcgccg
     5581 atgcggcgct cggtagcctg gctccagcgc ggatggcgca cctcggggtt cacgttggag
     5641 tagaacccgt actcctgcgg cgcgaacttg ttccacgcgg tcgcgggctc cttctcggtg
     5701 aggcggatgg tgacgatcga cttggcgctc ttgaagccgt atttc
//
LOCUS       DAOQNN010000197         5741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8164_length_5741_cov_9.919803, whole genome shotgun sequence.
ACCESSION   DAOQNN010000197 DAOQNN010000000
VERSION     DAOQNN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8164_length_5741_cov_9.919803"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="PKV97_10740"
     CDS             complement(<1..128)
                     /locus_tag="PKV97_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HNB06396.1"
                     /translation="MKTFSAKPAEVVHEWFVIDATDKVLGRVASEVALRLRGKHKA"
     gene            547..1050
                     /locus_tag="PKV97_10745"
     CDS             547..1050
                     /locus_tag="PKV97_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB06397.1"
                     /translation="MNHAKILLLKNMPIFHSVSDDGLEAILAGAATVEKSAGEYFFRQ
                     GEEALYMYVIESGSALVTKSWSDREAQLAQLGIGDCFGEMALISASGRSASVRAETPC
                     RAIEISNLNLQALKDHDVEQFIAVHLNISRELCRRLLLADEKRMAATEPRASTTEEPQ
                     LPWPPLM"
     gene            1279..1797
                     /locus_tag="PKV97_10750"
     CDS             1279..1797
                     /locus_tag="PKV97_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06398.1"
                     /translation="MPHAHADRHNGSDRADSGATRNRFIPTVTYRLNQPFPKPVPVAN
                     AGKCTVHIYDEDEAHATIDESTAQVQMKPGQHAITVDAPNQSASLKIHLRDKDDNVLY
                     IKGIAMKGVVGNGTGTVSDIGLFDCTSTDNYQTLILQEKITGQADFSFVLLFEDKKGN
                     IGVLDPRLTNNM"
     gene            complement(1865..5185)
                     /locus_tag="PKV97_10755"
     CDS             complement(1865..5185)
                     /locus_tag="PKV97_10755"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNB06399.1"
                     /translation="MSLPDLRHRLQRAIDLLQSEREALGDDVVELALKPLQEQWQRLE
                     AGAAGAGAGAGPQLRHLSVLFCDVVGSTSLSQYLSPEAVSAVMDDALAAFTHIAELNG
                     GSVLSYAGDSMLAVFGVPLAREDDAARAVRAGLAILAAASSRAQEVLDRHGHRGFNVR
                     AGVATGGVLLGGGVDGDSSARGMTVNLAARMEQTAAPGTLRVCPDTWRLLRGRFEGDE
                     QPPLMVKGRDEPIRTWLLRGERRGAAAVRAGVVGLQVPYVGRSAELAALQRAFAAFER
                     PAPGSSSLRPPMRQVVVLGDAGLGKSRLAAEFRAWAQRREGGVRWFTAVATEADAQRP
                     YGLLRRGFSRFAQLRDSDPPDTARHNWLAAYAPLLRSQGDAAVLGHLLGLDFSAHDEV
                     RPMLDEAQQLRDRAFHHALQALQAMAAGDAPLVVLLDDLHCADRGTLDFLDHLYTRAA
                     VLPLLMVGLGRPSLNEAHPGWLAQPGREQLTLQPLPGSDGEALADALLAPLQAAAPQL
                     RERLLGAAGGNPFFMEEWVQVLLDRGILVDDGERWQLREARLASWPVPGTLRGVLEAR
                     LDELDAGALAVLRAAAVVGMVFWDDVLAVLGHADVGDALAELQRRQFIVAHEGSDLEG
                     RFEYAFRHQTLQQVAYERVLERDRVTAHARIARWLAGLPGDKPLDRIAEHYERGGEPA
                     LACTAWLQAAQAAQARYANEQALAHADRALQHADLGDVRRRYDIQLVRQRVLGHLLDS
                     NQLTVALNELECLADALKSDRFRAEAKDCRARWLLEEGDASAARDVADRAWVLALESS
                     PEQAAKAGLTLVHSLTNLGQTADATALSEHVANLAARVGDKHTEGAVCNARGLIAEMS
                     GDYAQALSWYKKALEKHIETGNRCFEGSVVSNIGYVEMNLGEYESAEVYFRQASEVFL
                     RIGKRQHRSMVLLNLAMVSVNKGMLAMAKDYVQDATDSLDPKASRWMAATVQRVSGQV
                     ESELSNFTKALELLRSARDCFVALNMSALAIESQGFLAEALFRAGHVEQSRVEAFKVL
                     GSLESIGSAEGAEEPFRVYLILWNILKDVCRDRVAAVVSVGQALLQARAERISDRSRR
                     ESFLWRVPFHLALARLSL"
BASE COUNT         1079 a         2004 c         1766 g          892 t
ORIGIN      
        1 atggccttgt gcttgccgcg caggcggagg gcgacttcgc tggccacccg tccgagaacc
       61 ttgtccgttg cgtcaatcac aaaccactca tgcacgacct cggccggctt ggcgctgaaa
      121 gtcttcatgg caaatctcgt ttgcagggtt tgcggctgct tccgtcaccc tcggcgccgc
      181 ttatcggggc ggcctctcgg gcatccggac cgctcgcccg gagtgtccgg gcgggcgcct
      241 tttccgcgca gcctgaggcg gaaaagcctg tgactatacc atgctggccc gacgcgttca
      301 cagcgactct ccgcggtcgc cggcctgcac caggcctcag cgcatcgacg cctgccggca
      361 cggcaccgcg tcggccgccc acccttcggg ttccgcaggt tttcacgccc aggcctgcca
      421 ccccccggtc ggcggcgatc ttgcgtgcca aaatgaaaga aattactcga atcgcagggg
      481 actcgatcgg ccgtcgccag gctttccacg cacggcctcc gcctcctcgc accgccacgc
      541 cgcagcatga accacgccaa gatccttctc ttgaagaaca tgccgatctt ccattcggtc
      601 tcggacgacg ggctggaagc gatcctggcg ggtgcggcca ccgtcgagaa gagtgccggc
      661 gagtacttct ttcgccaagg cgaagaggcg ctttacatgt acgtgatcga gtccggcagc
      721 gccctggtca ccaagtcctg gtccgaccgc gaggcgcagc tcgcccagct cggcatcggc
      781 gactgcttcg gcgagatggc gctgatcagc gccagcgggc gcagcgcctc ggtgcgcgcc
      841 gagacgccct gccgtgccat cgagatctcc aacctgaacc tgcaggcgct gaaggaccac
      901 gacgtcgagc agttcatcgc cgtgcacctg aacatttcgc gcgaactctg tcggcgcctg
      961 ctgctggccg acgagaagcg catggcggcg acggaaccgc gcgccagcac gaccgaggag
     1021 ccacagctgc cctggccgcc gctgatgtga cgccggcggc cgcccggccg cccgatgcgc
     1081 agcacccgac acccggcaca agcacaaggc ctgcctccga cgacggtctt cgcggtgtgc
     1141 ggagctgcac gaatgccccc gttcccgcca agagcgcatg gacagcattt catatttttc
     1201 agatcatcgg caggccgaag cagtctgtcg gcaccaccgt tctcatcatg ctgttgaagt
     1261 ttctccagga ggaagcccat gcctcacgcc cacgccgacc gccacaatgg ttccgaccgc
     1321 gctgattccg gcgcaacgcg caatcgattc atcccaaccg tcacctaccg actcaatcag
     1381 cccttcccga agccagttcc ggtcgccaac gccgggaagt gcacggtgca catctatgac
     1441 gaggatgagg cgcacgccac gatcgatgaa tcgaccgctc aggtccagat gaaacccggc
     1501 cagcatgcga tcaccgtcga cgcgccgaat caatccgcca gcctgaagat tcacctgcgc
     1561 gacaaggacg acaacgtctt gtacatcaag ggcatcgcga tgaagggcgt ggtgggcaac
     1621 ggcacaggca ccgtcagcga catcggcctg ttcgactgca ccagcaccga caattaccag
     1681 accctgatcc tgcaggaaaa gatcaccggg caggcagatt tcagctttgt gctgttgttc
     1741 gaagacaaga aggggaatat tggggtgctg gatccacgtc tgaccaacaa catgtaacac
     1801 atcgattgat cacgcttatg cagcggctcc atatcggagc cgtctttatt caaagcctgg
     1861 ttgactacag actcagccga gcaagggcga ggtgaaatgg gactcgccac aagaatgatt
     1921 cacgcctaga tcggtccgag atccgctcag ccctcgcctg caaaagtgcc tgcccgacag
     1981 aaactacagc tgcgacgcga tcccgacata catccttcaa tatattccaa aggatcaggt
     2041 aaacccgaaa cggctcctca gctccttctg cggatccaat ggattcaagt gacccaagca
     2101 ccttgaatgc ctcgaccctc gattgctcaa cgtgccccgc gcggaacaag gcctccgcca
     2161 agaagccctg tgactcaatc gccaaggcac tcatatttaa tgcaacaaag cagtctcgcg
     2221 ccgaacgcag caactcaagt gccttggtga agttgctcaa ctcactttca acctgaccgg
     2281 acacgcgctg aactgttgca gccatccatc gacttgcttt cggatcgaga gaatcggtcg
     2341 cgtcttgaac gtaatctttc gccatcgcca acatgccctt atttacagaa accatggcga
     2401 ggtttagcaa aaccattgaa cggtgttgcc gcttaccaat ccgcaaaaac acctcagaag
     2461 cttggcgaaa gtacacttct gccgactcat actctccgag attcatctct acatacccaa
     2521 tatttgatac aacagatcct tcaaagcagc ggttcccggt ttcaatatgc ttttcaagtg
     2581 ccttcttata ccaactcagc gcctgagcat agtcaccaga catttcggca atcaacccgc
     2641 gcgcattgca aactgcacct tcagtgtgtt tatcgccaac acgagccgcc aaattagcaa
     2701 catgttccga aagagcggtt gcatccgcgg tttggccgag atttgtcaat gagtgcacca
     2761 gcgtaagacc agcctttgcc gcttgctcag gcgagctttc cagagccaaa acccacgcgc
     2821 gatcggcaac atctcgggcg gcagaggcat cgccctcttc cagcagccat ctcgcccgac
     2881 aatctttggc ttccgcccta aatctgtccg atttcaacgc atccgccaag cactcaagct
     2941 cattcaaggc caccgtcaat tggttggaat ccaacaggtg acccaatacc cgctggcgaa
     3001 caagctggat gtcatacctg cggcggacat cgcccaagtc agcgtgttgc agcgcccgat
     3061 ccgcatgcgc cagcgcctgt tcgttggcgt agcgagcctg ggcggcctgt gcggcttgga
     3121 gccaggcggt gcaggccagg gcaggctcgc caccgcgctc gtagtgttcg gcgatgcggt
     3181 cgagcggctt gtcgccgggc aggccggcca gccagcgggc gatgcgtgcg tgggcggtaa
     3241 cgcggtcgcg ttcgagcaca cgctcatagg cgacctgctg cagcgtctga tgccgaaagg
     3301 cgtattcgaa gcggccctcc aggtcactgc cctcgtgcgc gacgatgaac tggcgccgct
     3361 gcagttcggc cagcgcgtcg ccgacgtcgg cgtggccgag cacggccagg acatcgtccc
     3421 agaacaccat gcccaccacg gccgcggcgc gcaggacagc cagtgcgccg gcatcgagct
     3481 cgtccagacg ggcctcgagc acaccgcgca gcgtgccggg caccggccag gacgcgaggc
     3541 gcgcctcgcg cagctgccag cgttcgccgt cgtcgacgag gatgccgcgg tccagcagca
     3601 cctggaccca ctcttccatg aagaaggggt tgcctcctgc ggcgcccagc agccgctcgc
     3661 gcagctgggg agccgccgcc tgcagcggcg ccaacaatgc gtcggccagg gcctcgccgt
     3721 cgctgccggg caggggctgc agcgtgagct gctcgcgccc aggctgcgcc aaccagccgg
     3781 ggtgggcctc gttcaggctc gggcgcccca gcccgaccat cagcagcggc agcaccgccg
     3841 cgcgggtgta caggtggtcg agaaagtcca gcgtcccgcg gtcggcgcag tgcaggtcat
     3901 cgagcagcac caccaacggc gcgtcgcccg cggccatggc ctgcagcgcc tgcagggcgt
     3961 ggtggaaggc gcggtcgcgc agctgctggg cctcgtcgag catcggccgg acctcgtcat
     4021 gggccgagaa gtccaacccg aggaggtggc caagcaccgc cgcgtcaccc tggctgcgca
     4081 gcaggggagc gtaagcggcg agccagttgt ggcgcgcggt gtcgggtggg tcgctgtcgc
     4141 gcagctgggc aaagcggctg aagccgcggc gcagcagacc gtaggggcgc tgggcgtccg
     4201 cctcggtggc caccgcagtg aaccagcgca cgccgccctc gcgccgctgc gcccaggcgc
     4261 ggaactcggc cgccaggcgg gacttgccca ggccggcatc gccgagcacg acgacctgcc
     4321 gcatcggtgg gcgcaggctg ctggagcccg gtgccggacg ctcgaaggcg gcgaaggccc
     4381 gctgcagtgc ggcaagctcg gccgaccggc cgacgtaggg cacctgcagg ccgacaacac
     4441 ccgcgcgcac cgccgcggcg ccgcgccgct cgccgcgcag cagccaggtg cggatcggct
     4501 cgtcgcggcc cttgaccatc aggggcggct gctcgtcacc ctcgaagcgc ccgcgcaaca
     4561 gccgccaggt gtccggacag acgcgcagcg tgccgggcgc ggcagtctgc tccatccgag
     4621 cagcgaggtt gaccgtcatg ccacgggcgc tggagtcgcc atcgacccca ccgcccagca
     4681 acacaccgcc ggtggcgacg ccagcgcgca cgttgaagcc gcggtggccg tggcgatcga
     4741 gcacctcctg ggcgcgcgaa ctcgccgcag cgaggatcgc caggccggcg cgcaccgccc
     4801 gcgcggcatc gtcttcgcgc gccagcggca caccgaacac cgccaacatg ctgtcgccgg
     4861 cgtaggagag cacgctgcca ccgttgagct cggcaatgtg ggtgaaggcc gcgagtgcgt
     4921 cgtccatcac cgccgacacc gcctcgggtg acaggtactg actcagcgag gtcgagccga
     4981 ccacgtcgca gaacagcacg ctgaggtggc gcagctgcgg cccggccccg gcccctgcac
     5041 ccgcagcgcc cgcttcgagg cgctgccact gctcctgcag gggcttgagc gccagctcga
     5101 ccacatcgtc ccccagggcc tcgcgctcgg actgcagcag atcgatggcg cgctgaaggc
     5161 gatggcgcag gtcaggcagg ctcacggcag actctggacg gtgttctcga cagacgccgt
     5221 gcgaccaacg cgccggcatg cccggatggg acggcgcgcc gcccccgcga ccgcagcccg
     5281 gccgaggacc ttagcagccc accggcgtgg ccagggcgcc gcaaatgcaa cacattgcag
     5341 ccaacgcgat cggcggaggg aacggggaag aacctgcacc cggacggtgc accggcgccg
     5401 atccccgcac cgggtggaca gcggccagcc cgcaggccgt cgctcgttca cgacagggtc
     5461 cagggtggac cggcacggat cgcagcggca aaaaacaaac gcgccggcct tcacaggccg
     5521 gcgcgctccg ggtcagggtc cggtcgtcgc cgcatcaggc ggcaaaggcg ccgcccgagc
     5581 ggggagccgg gcggggccga ctcacttgcc ggacggcacc ttgccttcca cgcccttgac
     5641 gtagaagttg atgccgccga ggaacttgtc gtcggcgacc tcgtccttct tcagcacttc
     5701 cttgccgtcc tggccgacga tcgggccctt ccagatcacg a
//
LOCUS       DAOQNN010000198         5734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8175_length_5734_cov_8.680401, whole genome shotgun sequence.
ACCESSION   DAOQNN010000198 DAOQNN010000000
VERSION     DAOQNN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8175_length_5734_cov_8.680401"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1849)
                     /locus_tag="PKV97_10760"
     CDS             complement(<1..1849)
                     /locus_tag="PKV97_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB06400.1"
                     /translation="MNRRVGPVCLSAALAASTLPAIVRAGNLPDLESLATTPVYAASR
                     HYLRQVDSPASVTVITADDIRSFGYRTLGDLLRSVRGVHIYNDRNYDYLTLRGYSRAG
                     DYNAGVALQIDGMRINDPVYDGGLIGSEFPLDVGLIERVEYIRGPGSAEFGGNALFGV
                     INIVTRSAGGSSGLVVGLDGGSKGRAGANARFGKRFENGASLLLSASYLDVEGEDLRF
                     KPDRATGFPGGKVRDGDYDYSRRAFAKLEHGDFALTAGFGSRVKGDPAPYLAPYMFGQ
                     PSTYGREAEGFVNLRHVLDLSESNRLTTRLYYGHYDYLGHWPLDLESGRQTEHDVTRA
                     DWSGTELRLETLLGTDLRVVSGVEHQRDFRLSQHDMTKPDRQVYYDFGDKASRTSAYV
                     QADYDMTGRITLTGGLRLDRASPGDTGINPRLGLIYKPGSDTAWKFLYGRGTRNASPY
                     ERLGSLEGASLDAERVENAEITVEHYFAPRTRGVASLFGFHVHRVVKLIEDADSGGSY
                     YANTGEVRGRGIEFEIDHVADSGTHLRTSIGYNRVRNDERGAMTNSPRWLAKVNLSRP
                     LPWFGLRAGLEGQWTSAQHSYLGRVSSIGLVNLTLLRPLRSDGWEASASV"
     gene            complement(1846..3072)
                     /locus_tag="PKV97_10765"
     CDS             complement(1846..3072)
                     /locus_tag="PKV97_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB06401.1"
                     /translation="MVIDSNTTARTAMARRLERRGFAVQASPSGQTALDALRVARAEG
                     CKFDLCFVSARLANSSGLPTVESIRAEGLIDPAHLIVMVPIATRVEASSWHAVSDLQF
                     LSKPVLSAELCAVIESALAGRLHQASEEPAPATRGGNGLGLSVLLAEDHPVNASIAGA
                     ILGDLGCDHVWVQNGAQAVEALRTRAFDVVLMDCQMPEMDGFTATTRIREAEQARGAE
                     HPIPIIALTANAMQGDRERCIEAGMTDYLAKPFTKAQLRTALERQAGLALRPIEPAGL
                     DSLMDVPSGAAVLDPAILAEVPGIGSDGGALGRRIVGLFREETEKMLDRLGPAMSSGD
                     SSTISAIAHKIKSSSAAVGALRLSQMAATLERAAREQRTIGDGARMAGELSAAYEEAS
                     TALTGYLDGAMAGVHA"
     gene            complement(3173..4624)
                     /locus_tag="PKV97_10770"
     CDS             complement(3173..4624)
                     /locus_tag="PKV97_10770"
                     /inference="COORDINATES: protein motif:HMM:NF028461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB06402.1"
                     /translation="MNPVYKTSLKKKLTAVVVIATLVGLTVSYLVASVSQLNSQRMQT
                     LSQLEGAAEIISANSASAISFGDAAAAEKTLASLRNRADIMAAWITLPNGTLFAAYPD
                     RAVADPRMDKPVRDHLQPGDIGFSTMLTVSHPIMEERETLGYLTLRVDLSAMWTDLLK
                     QFAFSGAGTLLAFCLAYLFSLKLQREVATPILELAEASRSVARDRRYDRRVTHRSNDE
                     VGDLVVGFNDMMSQIESRDRELREHREQLEMQVLNRTAELLAAKEQAEAANRAKTQFL
                     ANMSHEIRTPMNGVLGMTDLLLDTELSDKQRRFAKTLRVSAESLLYIINDLLDFSKIE
                     AGKLELENVEFRPLLSAEEVAVLFAERAQAKGLELIVSVSDNVPRSVMGDPYRVKQIL
                     SNLISNAIKFTSAGEIEVRLAAMASERGDCLRFEVRDTGVGVGESARDRLFKAFSQAD
                     SSTTRKYGGTGLGLMIAKQLSELMGGGNRVRKS"
     gene            complement(4624..5280)
                     /locus_tag="PKV97_10775"
     CDS             complement(4624..5280)
                     /locus_tag="PKV97_10775"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNB06403.1"
                     /translation="MGGRLHSDADAGSRARLVRQGQGHVLSDVAMKPLRFRDLRRLAL
                     GTLLALASGTSGLQAQALSEAQVKAGFLFNFAKYVEWPAEAFYSKEAPVWLCVAGRDP
                     FGPAIDAFDGKQVHGRPVVVRRIAAGSDDFRGCHILYISDSEQRHVAPLLRSAQGAIL
                     TVSDIDGFIDVGGAIGLVSADERIQFEVNAATLQRSSLKASSQLLKLARNAGSVRGRN
                     "
     gene            complement(5202..>5734)
                     /locus_tag="PKV97_10780"
     CDS             complement(5202..>5734)
                     /locus_tag="PKV97_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB06404.1"
                     /translation="DLRSGRLLGTEMNFIPTPAGVVPYLLTSVETVNRLKGRTHGVEV
                     ALDWHPLKDLRLLATYAYSRERVIDGADGISAADYAGKTPRHVGSLRVSYNPMREWEL
                     DGWLRHVGKLVGNGIPAYTELDLRLAWRVAPALELSLVGQNLLDHRHPEWVGDYIPTP
                     TLEVERAWYVKAKVTF"
BASE COUNT          999 a         1910 c         1823 g         1002 t
ORIGIN      
        1 agaccgacgc gctggcctcc caaccgtcgc tgcgcagcgg tcgcagcagc gtcaggttca
       61 ccagtccgat gctcgacacc cggcccagat aggaatgctg cgccgaggtc cattgccctt
      121 cgagccccgc gcgcagtccg aaccacggca agggccgcga aaggttgacc ttcgccagcc
      181 agcgcggcga gttggtcatc gctccgcgct cgtcgttccg cacgcggttg tagccgatgc
      241 tggtgcgcag gtgcgtgccg gagtcggcaa cgtggtcgat ctcgaactcg atcccgcgtc
      301 cgcgaacttc tcccgtattc gcgtagtagg agccgcccga atcggcatcc tcgatcagct
      361 tcaccacgcg atggacgtga aaaccgaaca gcgacgccac gccgcgggtg cgcggcgcga
      421 agtagtgctc gaccgtgatc tcggcgttct ccacgcgctc ggcatcgagg gatgcgcctt
      481 ccagacttcc caatcgttca tacggtgacg cattgcgcgt gccgcgtcca tacagaaact
      541 tccatgcggt atcgctgccg ggcttgtaga tcaggcccag ccgcgggttg atcccggtgt
      601 cgcccggcga tgcacgatcc agcctgaggc cgccggtcaa cgttatccgc ccggtcatat
      661 cgtagtccgc ctgcacgtag gcactggtgc gcgaggcctt gtcgccgaag tcgtaataca
      721 cctgtcggtc gggcttggtc atatcgtgct gcgagaggcg gaagtcgcgc tgatgctcga
      781 cccctgacac cacgcgcaga tcggtgccga gcagggtttc gagccgcagc tcggtgcccg
      841 accagtcggc ccgggtcacg tcatgttcgg tctggcgccc cgattcgaga tcgagcggcc
      901 agtgacccag gtagtcgtaa tgaccgtagt agagacgcgt tgtcagccga ttcgactcgg
      961 acaggtcgag gacatgacgc agattgacga atccctccgc ctcgcggccg tatgtactcg
     1021 gctgcccgaa catgtacggc gccagatacg gggccgggtc gcctttcacg cgcgagccga
     1081 aaccggcggt caatgcgaag tcgccatgct cgagtttcgc aaaagcccgg cgcgaataat
     1141 cgtaatcccc atcgcggacc ttgccgccgg gaaaacccgt tgcccggtcc ggcttgaagc
     1201 gcaggtcttc tccctcgacg tcgagatagg aggccgacaa cagcaggctc gcgccgtttt
     1261 cgaagcgctt cccgaagcgt gcgttggcgc ccgcgcgacc tttgctgccg ccatcgagac
     1321 ccacgacgag gccactggat ccgcccgccg atcgcgtgac gatgttgatc acgccgaaca
     1381 gggcattgcc gccgaactcg gccgagccgg ggccgcgaat gtactcgacg cgctcgatca
     1441 ggccgacatc gagcgggaat tcggagccga tcaggccgcc gtcatacacc gggtcgttga
     1501 tgcgcatgcc gtcgatctgc agcgcgacgc cggcgttgta gtcgccggca cgcgagtagc
     1561 cgcgcagcgt caggtagtcg tagttgcggt cgttgtagat atgcacgccg cgcacgctgc
     1621 gcagcagatc gccgagcgtg cggtagccga agctgcgaat gtcgtccgcc gtgatgaccg
     1681 tgaccgaggc cggcgaatcc acctgacgaa ggtagtggcg cgacgccgcg tagaccggcg
     1741 tcgtggcgag agactcgagg tcgggcagat tgcccgcccg gacaatcgcc ggcagcgtgc
     1801 tggccgccaa cgcggcagac aggcaaaccg gaccgactcg ccggttcatg cgtgcacacc
     1861 tgccattgcc ccgtcgagat aaccagtgag cgctgttgac gcctcctcgt aagccgcgga
     1921 gagttcgccg gccatccggg ctccatcgcc gatcgtacgc tgctcgcgcg cggcccgttc
     1981 cagcgttgcc gccatctgcg acaggcggag cgccccgact gcggcgctcg acgacttgat
     2041 cttgtgcgca atcgcggaga tcgtggaact gtctccggaa ctcatcgcag ggccgaggcg
     2101 gtcgagcatc ttctccgtct cctcgcgaaa cagcccgacg atgcgacggc cgagcgcacc
     2161 gccgtcgcta ccgataccgg gcacctccgc aaggatcgcc ggatcgagga ccgccgcgcc
     2221 gctgggcaca tccatcagcg agtccaggcc ggccggctcg atcgggcgca gcgccagccc
     2281 ggcctgccgc tccagggcgg tccgcaactg cgccttggta aagggtttgg ccaggtaatc
     2341 ggtcatcccg gcttcgatgc aacgttctcg atcgccctgc atcgcattgg cggtcagcgc
     2401 gatgatcggg atcggatgct ccgctccgcg cgcctgctct gcctcgcgga tgcgcgtggt
     2461 cgcggtgaag ccgtccattt cgggcatctg gcagtccatc aggaccacgt cgaacgcgcg
     2521 ggtgcgcagg gcctcgacgg cctgcgcccc gttctgcacc caaacatggt cgcaaccgag
     2581 gtcgccgaga atcgcgcccg cgatgctcgc gttgaccgga tgatcctcgg caagcaagac
     2641 ggaaaggccg agtccgttgc cgccacgcgt ggccggcgcc ggctcctcgc tggcctggtg
     2701 gagacggccg gccagcgcgg attcgatcac ggcacacagt tcggccgaga gaacgggctt
     2761 ggacaggaac tgcagatcgg aaacggcgtg ccagctactt gcttccacac gcgtcgcgat
     2821 cggcaccatc acgatcagat gcgccggatc gatgaggcct tcggcccgga tcgattcgac
     2881 ggtcggcaat cccgaactgt tggcgagtcg tgccgatacg aagcacaggt cgaacttgca
     2941 cccttccgca cgcgccacgc gcaatgcgtc gagcgccgtc tgccccgatg gcgatgcctg
     3001 gacggcgaaa ccccggcgct cgaggcgcct tgccatcgcg gtgcgcgccg tcgtgttcga
     3061 gtcgatcacc atggcgcgaa tgccggcaag ctgtgccggc ccggcttcgc actcggtttc
     3121 atggccgatc ctttgcgaac cgatcgtgaa ccagaacgtc gagccggcac cttcacgact
     3181 ttcgaacccg atttcccccc cccatcagtt cggacaactg cttggcgatc atcaggccca
     3241 ggccggttcc gccgtatttt cgggtggtcg aactgtctgc ctgcgagaag gccttgaaca
     3301 gccggtcgcg ggccgattcg ccgacgccga caccggtatc gcgcacctcg aagcgcagac
     3361 aatcgccacg ctccgatgcc atcgcagcca ggcgcacttc gatctcgccc gcggaggtga
     3421 acttgatcgc attggaaatc aggttcgaga ggatctgctt gacgcggtag ggatccccca
     3481 tgacggatcg cggcacgtta tcactgaccg aaacgatcag ttcgagcccc ttggcctggg
     3541 cccgctcggc gaacagcacc gcgacttcct cggcagacag cagcggacgg aactcgacgt
     3601 tctccaactc cagcttgccg gcctcgatct tcgagaaatc gagcagatcg ttgatgatgt
     3661 agagcagcga ctcggccgac acccgcagcg tcttggcgaa gcgtcgctgc ttgtccgaca
     3721 gttccgtatc gagcagcagg tcggtcatgc cgagcacgcc gttcatcggc gtgcgaatct
     3781 cgtggctcat gttggccagg aactgcgtct tcgcgcggtt cgccgcctcg gcctgctcct
     3841 tcgcggcgag cagttccgcc gtgcggttca gcacctgcat ttcgagttgc tcgcgatgct
     3901 cgcgcagttc gcggtcgcgc gactcgatct gcgacatcat gtcgttgaag ccaacgacca
     3961 gatcgccgac ctcgtcgttc gaacgatgcg tgacacggcg gtcatagcgg cggtcgcggg
     4021 ccacgctgcg cgaggcctct gccagttcga ggatcggggt cgcgacttcg cgctggagct
     4081 tgagcgagaa cagataggcg agacagaacg ccagcagcgt tcccgccccc gagaacgcga
     4141 actgcttgag cagatcggtc cacatcgccg acaagtcgac gcgcagggtc aggtagccga
     4201 gcgtctcgcg ctcttccatg atcgggtgac tcacggtcaa catggtcgag aaaccgatgt
     4261 cacccggttg gagatggtcg cgcaccggct tgtccatgcg agggtcggcc acggctcgat
     4321 cgggataggc tgcaaagagg gttccattcg gcagcgttat ccatgccgcc atgatgtcag
     4381 cccggttgcg cagcgacgcc agcgttttct cggccgccgc ggcatcgccg aagctgatcg
     4441 ccgaggcgct gttggcgctg atgatctcgg cggccccttc gagctgcgaa agcgtctgca
     4501 tgcgctgcga attgagctgg ctgaccgatg cgaccaggta gctgacggtc aggccgacca
     4561 gcgtcgcgat gaccacgacc gcggtcagct tcttcttgag cgaggtcttg tacacgggat
     4621 tcatcagttc ctcccccgca cgctaccggc gttgcgcgca agcttcagca actgcgaact
     4681 cgccttgagg ctcgatcgct gcagcgtcgc ggcattcact tcgaactgga tgcgctcgtc
     4741 tgcgctgacc aggccgatcg cgccgccgac atcgatgaac ccgtcgatgt cgctgacggt
     4801 caggatcgca ccctgggcgc tgcgcagcag gggcgcgacg tgtcgctgct cggaatcgct
     4861 gatgtagagg atgtggcagc cgcgaaaatc gtcgctgccc gccgcgatgc gacgaaccac
     4921 gaccgggcgg ccgtgtacct gcttgccgtc gaatgcatcg atcgccggcc cgaaggggtc
     4981 gcgcccggcg acgcacagcc acactggcgc ttccttgcta tagaaggcct cggccggcca
     5041 ttcgacatac ttggcgaagt tgaacagaaa accggccttg acctgcgcct cgctgagggc
     5101 ctgtgcctgc aatcccgacg taccgctggc caaagccagc agtgtgccga gagcgagccg
     5161 tcgcaggtcc cggaatcgaa gtggtttcat cgcgacgtcg ctcagaacgt gaccttggcc
     5221 ttgacgtacc aggcgcgctc gacttccagc gtcggcgtcg gaatgtagtc gcccacccat
     5281 tctggatggc ggtggtcgag gaggttctgt ccgaccagcg acagttccaa ggccggcgcg
     5341 acacgccagg ccagccgcag atcgagctcg gtgtaggcag gaattccgtt tccaacgagc
     5401 ttgcccacat gtcgcagcca gccatccaat tcccactcgc gcatcggatt gtaggagaca
     5461 cggagcgaac cgacgtgtcg cggcgtcttc cccgcatagt ctgctgcgct gatcccgtcc
     5521 gccccgtcga tcacgcgctc gcgcgaatag gcgtaggtcg caagcaggcg aaggtctttg
     5581 agcggatgcc agtcaagggc gacttccacg ccatgggtgc gtcctttcag ccggttgacc
     5641 gtctcgacgc tcgtcaggag atacggaacg acgcccgccg gggtgggaat gaagttcatt
     5701 tccgtaccga gcaggcgacc gcttctcaaa tcgc
//
LOCUS       DAOQNN010000199         5700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8249_length_5700_cov_10.349513, whole genome shotgun sequence.
ACCESSION   DAOQNN010000199 DAOQNN010000000
VERSION     DAOQNN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8249_length_5700_cov_10.349513"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PKV97_10785"
     CDS             complement(<1..814)
                     /locus_tag="PKV97_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB06405.1"
                     /translation="MNSTQDKQRTLLIVEDDLALQKQMRWAFDASETVVASDRESAIA
                     QLRKHEPAVVTMDLGLPPAPDDVSEGFKLLREILTLAPDTKVIVLTGQHDRENAIKAV
                     GLGAYDFFAKPFEPELLNLTIERAFRMHDLQRENARLAALEGSPLSGLLTRDPGMLKV
                     CRTIEKLASASATVALLGESGTGKEILARGLHMRSARARERFVAINCAAIPDALLESE
                     LFGYEKGAFTGAVKQTLGKIETANGGTLFLDEIGDLPLALQAKLLRFLQERSI"
     gene            complement(818..2887)
                     /gene="prsK"
                     /locus_tag="PKV97_10790"
     CDS             complement(818..2887)
                     /gene="prsK"
                     /locus_tag="PKV97_10790"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HNB06406.1"
                     /translation="MLEVAIWSYGVAAVAFLIFALYIFLAWRGALPGGALFAAVAISG
                     LWAGLSALEARGAWPFSGALAVALDLLRGAAWYVFLIVLSRPLWGRWMRWPAYVALAV
                     VVAQVLALALESAGVTGGLPIAPAIGAWLMHAIVGLMLVEQLYRGMPAVSRWGLKPLC
                     LALAAGYIFELYLFADALLFSRIDADVWAARGIAHALLIPLIAIAGTRSPSWTLRMSV
                     SREIVFHSTALAGAGLYLLVIAAAAYYVRFFGGDWGRALQMALLFAGLVVLGALLFSG
                     AVRARLRVFISKHLFPYRYDYRSEWLRFTQALSTVDRSLDLGQSVIKALSDLVESPGG
                     SLWLADAYGKCRPHSRFNQPPIDVVEALDSPLCGFLARSGWVINLEEYRVQPGRYQGL
                     VLPDWLSRLDEAWLVIPLQTAERLIGFVVLGSPRTPFDIDWEVLDLLKTAQRQAAGYL
                     DRMLAAEALLEARKFDSFNRMSAFVVHDLKNLVAQLSLMLKNAQRHRDNPEFQQDMLD
                     TVANVEARMRGLMTQLQEKRSIDPPRAVDLPALLRAVCESKRGQSPAIELVCAAAGPV
                     EVSAHPQRLERIIGHVVQNALDATAQDGKVVLSLAAGKDGRANVVVEDDGCGMSPEFV
                     RERLSRPFQTTKSSGMGIGVYETRQYLEEIGGALHYDSEVGRGTRVSIELPRIRREDG
                     QSTAMGI"
     gene            complement(2966..4351)
                     /locus_tag="PKV97_10795"
     CDS             complement(2966..4351)
                     /locus_tag="PKV97_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNB06407.1"
                     /translation="MLKVFSHYLPTHTLQQVLFDALTLFAVVLAAVAVLVTPTSAVDW
                     AICIPSALTFAASMIALNAAMGLYRPVYQRSHRQTFARVALSLTVSVPVAYLVFGLLP
                     WSEIAPHSLQLSVLLLLCSLLLVRGLVNQRQASALFVPRVLIVGTGRDARMVQQDLVR
                     PLQRSVEVVGFLPVGGNERVEVEQHAVLPPGSLLEVVRNLRVDEVIVAVRERRGGVLS
                     LRELLDCKLLGTRILDLSTFFERVQGQVRLDSLRASWLIYGDGFRQGWTRTFVKRCFD
                     LVVGTALLLVALPIMVLTALLILLEDGAPIFYSQERVGRGGKPFRVIKFRSMRRDAEK
                     DGKPRWATSNDDRVTRVGRFIRKLRIDELPQLFNVLKGDMSLVGPRPERPYFVDQLTQ
                     QIPFYAVRHCVKPGVTGWAQVRYQYGASVDDAAEKLQYDLYYVKNHSLILDTLVLFET
                     VRVVLTGEGAH"
     gene            4656..5105
                     /locus_tag="PKV97_10800"
     CDS             4656..5105
                     /locus_tag="PKV97_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNB06408.1"
                     /translation="MTQAQRDFSEVYGLSFEDLHIGRTASVSRTVSEADILMFAGVSG
                     DTNPVHLDEEFAASTMFGGRIAHGMLSAGLISAVFGTRLPGPGCIYLSQSLKFKAPVK
                     IGDTVVARVTVKELKAEKRRAVFSTVCTVGDKVVMDGEAEILVPART"
     gene            5261..5698
                     /locus_tag="PKV97_10805"
     CDS             5261..5698
                     /locus_tag="PKV97_10805"
                     /inference="COORDINATES: protein motif:HMM:NF024932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNB06409.1"
                     /translation="MDVVIPRRSRRTHSEAFKQSVVSACREPGVSVAGLALANGLNAN
                     RVHRWMRECGIEPPSRRKPLCAERTPLAEPGFVPVQVAPAVETLVIRLEAQRGNARIK
                     LEWPLQAAARRHRSTGCRCLAPPAHRAGARARVAPDQDRAAHA"
BASE COUNT         1092 a         1919 c         1807 g          882 t
ORIGIN      
        1 cgatcgagcg ctcctgcagg aaacgcagca gcttggcctg cagcgcgagc ggcaggtcgc
       61 cgatctcgtc gaggaagagg gtacccccgt tggccgtctc gatcttgccg agggtctgtt
      121 tcactgcgcc ggtgaaggcg cccttctcat agccgaacag ctcgctctcg agcaaggcat
      181 cgggaatggc ggcgcagttg atcgccacga agcgctcgcg ggcgcgggcg gagcgcatgt
      241 gcaggccgcg cgcgaggatc tccttgccgg taccgctctc gccgagcagc gcgaccgtgg
      301 cggaggccga ggcgagcttc tcgatcgtgc ggcagacctt gagcatcccg ggatcgcggg
      361 tgagcagtcc ggagagcggg ctgccctcga gcgcggcgag gcgggcgttc tccctctgca
      421 ggtcgtgcat gcggaaggcg cgctcgatgg tgagattgag cagctcgggc tcgaagggct
      481 tggcgaagaa gtcgtaggcg cccaggccca ccgccttgat cgcgttctcg cggtcatgct
      541 ggcccgtgag cacgatgacc ttggtatcgg gcgcgagcgt gaggatctcg cgcaggagct
      601 tgaagccctc gctgacgtcg tcgggcgccg gcggcaggcc gaggtccatg gtcaccaccg
      661 caggttcgtg tttgcgcagc tgcgcgatcg cgctctcgcg gtcgctcgcg accacggtct
      721 cgctggcgtc gaacgcccat cgcatctgct tttgcagcgc gaggtcgtcc tcgacgatca
      781 ggagagtacg ctgcttgtct tgcgtcgaat tcacgttcta tatgcccatc gcggtagatt
      841 gcccgtcctc gcgccggatg cgggggagtt cgatactgac gcgggtgccg cgcccgactt
      901 cgctgtcgta gtgcagcgcg ccaccgatct cctcgaggta ctgccgggtc tcgtagaccc
      961 cgatccccat cccgctcgac ttggtggtct ggaagggacg ggacagccgt tcgcggacga
     1021 attcaggact catcccacag ccgtcgtcct cgacgaccac gttcgcccgc ccgtccttcc
     1081 cggcagcgag actgagaacc accttaccat cctgtgccgt ggcatccagt gcattttgca
     1141 cgacatgacc gatgattctc tccagccgct gcgggtgcgc actgacctcc accggtcccg
     1201 cggctgcgca cacgagctcg atcgcgggcg actggccacg cttggactcg cacaccgcgc
     1261 gcagcagcgc ggggagatcc accgcgcgcg gcgggtcgat cgagcgcttc tcctgcaact
     1321 gcgtcatcag cccgcgcatg cgcgcctcga cgttcgccac cgtatccagc atgtcttgct
     1381 ggaactcggg gttgtcgcgg tggcgctggg cgttcttgag catcagcgac aactgggcga
     1441 cgaggttctt caggtcatgg accacgaagg ccgacatgcg gttgaaggag tcgaacttgc
     1501 gtgcctccag caaggcctcg gccgcgagca tacggtccag atagcctgcg gcctgacgct
     1561 gcgccgtctt gaggagatcg aggacttccc agtcgatgtc gaagggggtc cgcggcgaac
     1621 ccagtacgac gaagccgatc aggcgctcgg cggtctgcag cgggatcacc agccatgcct
     1681 cgtcgagccg tgacagccag tccggcagca ccagaccctg gtagcgtccg ggctgcacgc
     1741 gatactcctc gaggttgatc acccagccgc tgcgggcaag gaacccgcac agcggcgaat
     1801 cgagcgcctc gaccacgtcg atcgggggct ggttgaagcg cgaatgcggc cggcacttgc
     1861 cgtaggcatc ggccagccag agcgaccctc cagggctctc gacaagatcc gacagcgcct
     1921 tgatgaccga ttgacccaga tcgagactgc ggtcgacggt cgacagggcc tgggtgaagc
     1981 gcaaccactc gctccggtaa tcgtagcggt acggaaagag gtgcttgctg atgaagaccc
     2041 gcaggcgcgc gcgcacggcg cccgaaaaca gcagcgcgcc gaggaccacc aggccggcga
     2101 agagcaatgc catctgcagt gcgcgccccc aatcgccgcc gaagaagcgc acgtaatagg
     2161 ccgcagcggc gatcacgagc aaatagaggc cggcgccggc gagcgcggtc gaatggaaga
     2221 cgatctcgcg cgatacggac atccgcaggg tccatgaggg actgcgcgtc cccgcgattg
     2281 cgatcagcgg gatgagcagg gcgtgcgcga tgccgcgcgc tgcccagaca tccgcatcga
     2341 tgcgcgagaa caggagggca tcggcgaaga ggtacagttc gaagatgtag cccgccgcca
     2401 gcgcgaggca aagcggcttc aaaccccagc gcgagacggc gggcatgccc cggtagagct
     2461 gttccacaag catcaggccg acgatcgcgt gcatcagcca ggcaccgatc gccggcgcga
     2521 tcggcaaccc tccggtgacg cccgccgact cgagcgcgag cgcgagcacc tgcgccacca
     2581 ccaccgcgag cgccacatac gccggccagc gcatccaccg cccccataac gggcgggaca
     2641 gcacgatcag gaacacgtac caggccgcgc ccctgagcag atcgagcgcg acggcgaggg
     2701 caccggaaaa cggccaagcg cccctggcct cgagcgccga aagacccgcc cacagaccgg
     2761 aaatcgccac ggccgcgaac aaggccccac ccggcagcgc gccccgccac gcgaggaaga
     2821 tgtacagcgc gaagatgagg aaggctacgg ccgcaacccc ataactccag atcgcaacct
     2881 caagcacgcc cctctcccct gtcacgcatt cgtgcgaact gcgccgtacc cccgtccaag
     2941 cgagcccgca gtctcacgcg gccgctcagt gcgcgccttc accggtcagg acgaccctca
     3001 ccgtctcgaa gaggacaagg gtatccagga tcagcgagtg gttcttcacg taatacagat
     3061 cgtactggag cttctccgcg gcatcgtcga ccgaagcccc gtactggtaa cgaacctgcg
     3121 cccacccggt gacgccgggc ttgacgcagt gccgcaccgc atagaacgga atctgctggg
     3181 taagctggtc cacaaaatag gggcgctccg ggcgcgggcc gaccagactc atgtcgccct
     3241 tgagcacgtt gaacagctgc ggaagctcat cgatacgcag cttgcggatg aaacgcccga
     3301 cgcgtgtcac tcgatcgtcg ttcgaagtcg cccaccgcgg cttgccgtcc ttctcggcgt
     3361 cacgccgcat gctgcggaac ttgatgacac ggaacggctt cccaccgcgt cccacacgct
     3421 cctgcgagta aaagatcggc gcgccatcct ccagcaggat caacagcgca gtgagcacca
     3481 tgatcggcaa ggccacgagc aggagcgcgg tcccgacgac gaggtcgaaa caacgcttga
     3541 cgaaggttcg cgtccagcct tggcggaagc cgtcaccata gatcagccag ctcgcccgca
     3601 gcgaatccag cctcacttgc ccctgcacgc gctcgaagaa ggtcgacaga tcgaggatgc
     3661 gcgttccgag caacttgcag tccagcagtt cgcgcaagga gagcactccc ccccgacgtt
     3721 cgcgcaccgc gacgatcacc tcgtccacac gcaggttgcg caccacctcc agcaagctgc
     3781 ccggtggcaa cacggcgtgc tgctccacct cgacccgttc gtttcccccc accggaagaa
     3841 agccgaccac ttccacgctg cgctgcagcg gacggaccag gtcctgttgc accatgcgcg
     3901 cgtcacggcc ggtacccacg atcagcacgc gcggcacgaa aagggcagag gcctggcgct
     3961 gattgaccag cccgcggacc agcaacagcg aacagagcaa cagcaatacg ctgagctgca
     4021 gggagtgcgg ggcaatttcg gaccacggca acagcccgaa cacgaggtag gccactggta
     4081 cgctcacggt cagcgacagg gcgacgcgcg cgaaggtctg ccgatgggaa cgctgataca
     4141 ccggtcggta caaacccatc gccgcattga gggcgatcat gcttgcagcg aaggtaagcg
     4201 ccgatggaat gcagattgcc cagtcgaccg cgcttgtcgg cgtcacgagt accgcaaccg
     4261 ctgcaagcac caccgcaaaa agcgtcaagg cgtcgaacag cacctgctgc agggtgtgcg
     4321 tgggaaggta gtggctgaac accttcagca tcgaggggcc tctgcatgtg ttgggaaggg
     4381 tcggaaccct gggcgccggg agtgcttatc gcggagggat cgcccggctc ctgatcgtgc
     4441 ctaagcttag acaatcaccg gcaggacacc tgtgactcac ttcacggcga gggcgggtgg
     4501 agcaaaccgc ctcgagcaca ccgagcaaaa tcgcacgaaa cgcccgcctg gccttgcggc
     4561 aaatgcggca gcgcagcatc caaggttgat tttagcatgc gaagcttgta gcgtcggtcc
     4621 gttaccggag caccgtttca cacaaggaga agtacatgac tcaagctcaa cgcgatttca
     4681 gcgaagtcta cggcctcagt ttcgaggacc ttcacattgg ccgtaccgcc tcggtctcac
     4741 gtacggtgag cgaggccgac atcctgatgt tcgccggcgt atcgggcgac accaacccgg
     4801 tccatctgga cgaggagttc gctgcgagta cgatgttcgg cggccggatc gcccacggaa
     4861 tgctctccgc cggcctcatc tcggccgtct tcggcacccg tctgcccggc cccggctgca
     4921 tctacctgtc ccagagcctg aaattcaagg cgcccgtcaa gatcggtgac accgttgtcg
     4981 cgcgtgtcac cgtcaaggaa ttgaaggcag agaagcgccg cgcggttttc tcgaccgtct
     5041 gcacagttgg agacaaggtg gtcatggatg gcgaggctga aatcctcgta ccggcgcgca
     5101 cctgatcgag catgccctcg cctcgcgcgg ctccctgcag aggccgtgcg aggcacctgc
     5161 agaaatctcg ccacccagct ctaagccccc ggcgaaccca ccttccgccg tctgcttatc
     5221 ccattgaaga tcgtgtccat caagttcgat ggtggacacg atggatgtgg ttatcccgcg
     5281 ccggagtcgg cgcacgcaca gcgaagcctt caagcagtct gtggtctcgg cctgtcgtga
     5341 gcccggcgtc tcggtggccg gcctggctct ggccaacggc ctgaacgcca accgggtcca
     5401 ccgctggatg cgcgagtgcg gcatcgagcc gccgagtcgt cgcaagccgc tctgcgctga
     5461 acgcacgcca ttggctgagc cgggctttgt acccgtgcag gtggctcccg ccgtcgaaac
     5521 gttggtgatc cgcctcgaag cgcagcgtgg caacgcccgc attaagctcg aatggccgct
     5581 ccaggccgct gcccgccgac atcgatcaac tggatgccga tgccttgcgc cacctgctca
     5641 tcgggcagga gcgcgagctc gtgtggcgcc agaccaagat cgagcggctc acgcatgagc
//
LOCUS       DAOQNN010000200         5700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8250_length_5700_cov_8.914969, whole genome shotgun sequence.
ACCESSION   DAOQNN010000200 DAOQNN010000000
VERSION     DAOQNN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8250_length_5700_cov_8.914969"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PKV97_10810"
     CDS             <1..235
                     /locus_tag="PKV97_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06410.1"
                     /translation="QHGVVSSCGFGCGRFRHTLIEHPSCQRNPSLIYQILALSMQQWE
                     RSVAGSGAALEVLHLLQLLHLLHTRTVMEHAPK"
     gene            complement(292..1491)
                     /locus_tag="PKV97_10815"
     CDS             complement(292..1491)
                     /locus_tag="PKV97_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNB06411.1"
                     /translation="MQNKLSSHRRTRSATTALLPSALGAALLALAATPAQAIGFSQGD
                     ATLDINGTVNGFYSHRTEERAGVKTTDSALTNGLLPGWINFVFTTKVEGLDIKAHVGF
                     APGINDKSDVVGLPSDPSCGGSTGCDSPFTQIDTRNLYFQFGNTEWGTVKFGRDIGLF
                     GQKIILSDMTLLGLGGNSYASTPFNTTFGMIGHGYMYTGFQPQITYTTPAFGGFSASA
                     GIFDPSKFAGDETKDPGFQAMANYDWKAGTAKGSVWAGAVHQRTSGAGSFDANGFELG
                     AKVGIGAFEAVAYGFDASGLGLSTVGALYLSPFGKTDGKGYFVQGTYTVGKTKFGINF
                     GENRDSGGALADTNKFRSATVGVYHSLNKYITLVGEYNQERGDGDDLFAGDLKTRTIS
                     LGGIIMF"
     gene            1755..2765
                     /locus_tag="PKV97_10820"
     CDS             1755..2765
                     /locus_tag="PKV97_10820"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNB06412.1"
                     /translation="MKEPPSPPVAPSPPVAPSPFQAHAETRSGAVARPASGRFPLAII
                     GPGALGLHFAARLDGIAPVALIARDAAGAARLNAGVEVGGHLFHPNAFAAERAPEADW
                     VIVLVKADDTAAAASTAARMRPLGVLSLQNGLTGELLRAHCGAERVDQGITTEGAYRE
                     RDDHGERVYPSGAGETLVPPGFEAVAELLVRAGLRARVEPDIAPARLAKLLVNVAINP
                     LAALFRVPNGALLDPPHRVLLDTLVREALPVLHAHGLELDEDAALARVHAVARATAAN
                     RASMLQDLLAGRRTEIDAITGALLRMAARTGTELPTHRAVEVLVRALESRNAERTDRV
                     GC"
     gene            2966..3739
                     /gene="rfbF"
                     /locus_tag="PKV97_10825"
     CDS             2966..3739
                     /gene="rfbF"
                     /locus_tag="PKV97_10825"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNB06413.1"
                     /translation="MKAVILAGGLGTRISEETVNRPKPMVNIGGRPILWHIMKSYSSH
                     GIHDFVICCGYMGYVIKEYFANYFLHMSDVTFDMSDNSMEIHERHAEPWRVTLVDTGE
                     NTQTGGRLARVAKYLRNEEAFCFTYGDGVADIDLGAEIDFHKAHGKLATVAAVQPPGR
                     FGALELKGDDVDAFVEKPLGDGGFINGGYFVLSPEVLDYITEGDQTVFEGSVLKKLIA
                     DQQLAAYRHTGFWRPMDNLRDKIMLEDLWQRGEAPWKTW"
     gene            3724..4791
                     /gene="rfbG"
                     /locus_tag="PKV97_10830"
     CDS             3724..4791
                     /gene="rfbG"
                     /locus_tag="PKV97_10830"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HNB06414.1"
                     /translation="MENLVSPAFWRGRRVFVTGHTGFKGGWLSLWLQSMGADVCGFAL
                     PPAAAPALFHVADVERGMHSVLGDVRDYERLRQALAAARPEIVLHLAAQPLVPYSYAE
                     PVETFSTNAMGTVNLLEACRHQPDLKAVVVVSSDKCYENREQLWGYRETDPMGGHDPY
                     SASKGCTELVVASYRRSFLAARGVALASARAGNVIGGGDWTPSRLVPDVLAAFARNEA
                     VVLRNPDAIRPWQHVLEPLAGYLLLAQHLVEHGEAFAEGWNFGPDETDARTVAWIVEM
                     LAAGWGAGAAWQPSGEPRIHEAHTLKLDCTKARVRLGWRPRWQAEDAVTRSLAWYQAW
                     RAGADMHRYTLDEISAFTASS"
     gene            4788..>5700
                     /gene="rfbH"
                     /locus_tag="PKV97_10835"
     CDS             4788..>5700
                     /gene="rfbH"
                     /locus_tag="PKV97_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNB06415.1"
                     /translation="MSLIRKTLTPDADGKLLLDLPDLAGQPLEVIVRPARPTNAAQAW
                     RAGIDPVPASGKVVGDAEKDAMIDAALDAWLTTGRFNDAFEKRLASFVGTRFALTVNS
                     GSSANLVAFSTLTSPTLGDRAIRPGDEVITVAAGFPTTVNPILQNGAVPVFVDVCLPT
                     YNIDVTMLEAALSPRTKAIMIAHTLGNPFDLDAVCAFAEQHGLWLIEDCCDALGSTWK
                     GRKVGTFGHLATLSFYPAHHITMGEGGAVLTNDPQLRQIAESFRDWGRDCWCKPGCDN
                     TCGKRFGWQLGSLPAGYDHKYTYSHIGY"
BASE COUNT          945 a         1955 c         1842 g          958 t
ORIGIN      
        1 acagcatggt gtcgtctcct cgtgtggttt cggttgcggg cggtttcgcc acacgttgat
       61 tgagcatcct tcgtgccagc gcaatccatc ccttatttat cagatacttg ccctatccat
      121 gcagcaatgg gagcgctccg tggcgggttc tggagcagcg ctcgaagtgc tccacttgct
      181 ccagctgctc catctgctcc atacccgtac tgtcatggag cacgcgccga aatgaagcga
      241 ggcagcgcga ccttcgtcgc cctgccccgc aggctgggaa agttgcgcgc gtcagaacat
      301 gatgatgccg cccagcgaga tggtccgggt cttcaggtca cccgcgaaca ggtcgtcgcc
      361 atcgcccctc tcctggttgt attcgcccac cagggtgatg tacttgttga ggctgtgata
      421 cacgccgacg gtggcggaac ggaacttgtt ggtgtcggcc agcgcgccgc cgctgtcgcg
      481 gttctcgccg aagttgatgc cgaacttggt cttgcccacg gtgtaggtgc cctgcacgaa
      541 gtagcccttg ccgtcggtct tgccgaaggg cgacaggtac agcgcgccga cggtcgacag
      601 gcccagcccg ctggcgtcga agccgtaggc gaccgcctcg aaggcgccga tgccgacctt
      661 ggcgccgagc tcgaaaccgt tggcgtcgaa gctgcccgcc ccgctggtgc gctggtggac
      721 cgcccccgcc cacaccgagc ccttggcggt acccgccttc cagtcgtagt tcgccatggc
      781 ctggaagccg gggtccttgg tctcgtcacc ggcgaacttg ctgggatcga agatccccgc
      841 cgaggccgag aagccgccga aggccggcgt ggtgtaggtg atctggggct ggaagccggt
      901 gtacatgtag ccgtggccga tcatgccgaa ggtggtgttg aagggcgtcg acgcgtagct
      961 gttgccaccg aggccgagga gcgtcatgtc ggacaggatg atcttctgcc cgaagaggcc
     1021 gatgtcgcgg ccgaacttga ccgtgcccca ctcggtgttg ccgaactgga aatacaggtt
     1081 gcgggtgtcg atctgggtga aggggctgtc gcagccggtg gaaccgccac agctggggtc
     1141 gctgggcagg cccaccacgt ccgacttgtc gttgatgccg ggcgcgaagc cgacgtgagc
     1201 cttgatgtcc aggccctcca ccttggtggt gaacacgaag ttgatccagc ccggcagcaa
     1261 gccgttggtg agcgcggagt cggtggtctt gacgcccgcg cgctcctcgg tccgatgaga
     1321 atagaagccg ttgaccgtgc cgttgatgtc gagcgtggcg tcgccctgcg aaaacccgat
     1381 cgcctgggcc ggcgtggcgg cgagcgcgag cagcgcggcg ccgagcgccg acggcagcag
     1441 ggcggtggtg gcggatcggg tgcggcggtg gctggaaagc ttgttttgca tggatgtccc
     1501 ctcggatggt gtggatgcct tcgatccgcc cgcccggcgg ccggatcgaa ggcgggctgc
     1561 gcaaacgctg tgccaccccc ccaaaacgcg gcctgttccg ggcatttgtg cgcaacgcag
     1621 gcgcacagtc cgctccggat gctccacaat ccctgcccgc cctgcttcgg aatggcacct
     1681 ccggagcagg cgccggccgc aggcgagcga cgcccctgcg gcctgcaaga ctccctgcca
     1741 ggatctccgc tgcgatgaaa gagcccccct ctccgccggt cgcgccctcc ccgccggtcg
     1801 ccccctctcc cttccaggcg cacgccgaga cgcgatccgg cgcggtcgcg cgcccggcaa
     1861 gcggccgctt tccgctcgcg atcatcggcc ccggcgcgct cggcctgcac ttcgctgcgc
     1921 gcctcgacgg cattgcgccg gtcgcgctga tcgcgcgcga cgccgccgga gccgcgcgcc
     1981 tgaacgccgg cgtcgaggtg ggcggacacc tcttccatcc aaacgccttc gccgccgagc
     2041 gggccccgga ggcggactgg gtgatcgtgc tggtgaaggc ggacgacacc gccgccgccg
     2101 cgtccaccgc cgcacgcatg cgcccgctgg gtgtgctctc gctgcagaac ggcctgaccg
     2161 gcgagctgct gcgcgcgcac tgcggggcgg agcgggtcga ccagggcatc accaccgaag
     2221 gcgcctaccg cgagcgggac gaccacggcg agcgggtgta cccctccggc gcgggcgaga
     2281 ccctggtgcc gcccggcttc gaggcggtgg ccgagctgct cgtgcgcgcg ggcctgcgcg
     2341 cgcgcgtcga acccgacatc gcgcctgccc gcctcgccaa gctgctggtc aatgtcgcga
     2401 tcaacccgct cgcggcgctc ttccgcgtgc ccaacggcgc cctgctcgat ccgccgcacc
     2461 gcgtgctgct cgacaccctg gtgcgcgaag ccctgccggt gttgcacgcg cacggcctcg
     2521 aactcgacga ggacgccgcc ctcgcccgcg tgcacgcggt cgcccgcgcc accgcggcca
     2581 accgggcgag catgctgcag gacctgctcg ccggccggcg caccgagatc gacgcgatca
     2641 ccggcgcact gctgcgcatg gccgcgcgga cgggaacaga actgcccacg catcgggcgg
     2701 tggaggttct ggtgcgggcg ctggaatcac ggaacgcgga aaggacggat cgggtggggt
     2761 gctgaccggt gggaaatcat gtgcgcgcgc aaatgaaagc cttgccccca accactcaac
     2821 cccgctttct ccgtcatcgc ttgtcgagaa ccgactgtgg tcgatgcact tcaccagaac
     2881 agaccgcgcg gaccccctct attcggggcg atcagtctcg actcgggcgg tatcatgcga
     2941 ccggatccaa cacgaagaac ctctgatgaa agccgtgatc ctcgcgggtg gcttgggcac
     3001 ccgaatcagc gaagaaaccg tcaatcgccc caaaccgatg gtgaatatcg gcggccgtcc
     3061 gatcctctgg cacatcatga agtcctattc cagccacgga atccatgact tcgtgatctg
     3121 ctgcggctac atgggctatg tcatcaagga atacttcgcc aattacttcc tccacatgtc
     3181 ggatgtgacc ttcgacatgt ccgacaacag catggagatt cacgagcgcc atgccgagcc
     3241 ttggcgcgtg acgctcgtgg acacgggcga gaacacgcag acaggcggac ggctggcccg
     3301 cgtcgccaag tacctgcgga acgaggaggc gttctgcttc acgtatggcg acggcgtggc
     3361 agacatcgac ctcggcgcgg agatcgactt ccacaaggcc cacggcaagc tggccacggt
     3421 cgcggccgta cagccacccg gccgcttcgg cgcactggag ttgaaaggcg acgacgtcga
     3481 cgctttcgtc gaaaaacctc tgggcgacgg cggctttatc aatggcgggt acttcgtcct
     3541 ctcgcccgaa gtgctcgact acatcaccga gggcgaccag acggttttcg aaggatccgt
     3601 gctcaagaag ctgatcgccg atcagcaact tgcagcctac cggcataccg gtttctggcg
     3661 accgatggac aacctgcgcg acaagatcat gctcgaggat ctttggcagc gcggcgaggc
     3721 accatggaaa acctggtgag ccctgctttc tggcgggggc gccgcgtctt cgtcacgggt
     3781 cataccggct tcaagggcgg ttggctcagc ctgtggctgc agagcatggg ggcggatgtt
     3841 tgcggcttcg cattgccgcc cgccgccgcg ccggccttgt tccatgtcgc agatgtcgag
     3901 cgcggcatgc actccgtgct cggcgatgtg cgggactatg aaaggttgag acaggcactc
     3961 gcggcagcaa ggccggaaat cgtgcttcat ctcgccgcgc aaccgctcgt gccctactcg
     4021 tacgccgaac ctgtcgagac cttctcgacc aacgccatgg gcaccgtgaa tctgctcgag
     4081 gcctgtcggc accagcccga cctgaaagcc gtcgtcgtgg tcagcagcga caagtgctac
     4141 gaaaaccggg agcaactctg gggctatcgt gagacggatc cgatgggcgg acacgacccg
     4201 tattcggcca gcaagggctg cactgaactc gtcgtcgcgt cgtaccgtcg ctccttcctc
     4261 gccgctcgcg gcgtggccct cgccagcgcg cgggccggca acgtgatcgg aggcggagac
     4321 tggaccccca gccgtctggt gcccgacgtc ctggcggcct ttgcacgcaa cgaagccgtt
     4381 gttctgcgca atcctgacgc gatccggccc tggcaacacg tgctggagcc cttggcgggt
     4441 tatctgctct tggcccagca cctcgtcgag catggagagg ccttcgccga gggctggaac
     4501 tttgggcccg acgaaaccga cgcgcgtaca gtcgcgtgga tcgtggagat gcttgccgcc
     4561 ggctggggcg ccggtgccgc ctggcagcca tccggcgagc cgcggatcca tgaggcgcac
     4621 accctcaagc tggactgcac caaggcccgc gtccggctgg gctggcggcc tcgctggcaa
     4681 gcggaagacg cagtcacccg cagcctcgcc tggtatcaag cctggcgtgc cggtgccgat
     4741 atgcatcgat acactctcga cgaaatctcc gcattcacag ccagctcatg agcctgatcc
     4801 gcaagaccct cacgcccgat gctgacggca agctattgct cgacctgccc gatctggccg
     4861 gtcagccact cgaagtcatc gtccgcccgg cccgcccgac aaacgccgcg caggcctggc
     4921 gcgcgggcat cgaccccgtc cccgcatcgg gcaaggtcgt cggcgacgcc gagaaggacg
     4981 cgatgatcga cgccgcgctc gacgcctggc tgaccaccgg tcgcttcaac gacgccttcg
     5041 agaaacgcct ggcaagcttc gtcggcacgc gcttcgccct caccgtaaat tccggatcct
     5101 cggctaacct ggtggcattc tcaaccctca cctcgcccac gctcggcgat cgggcaatcc
     5161 gcccgggaga cgaggtgatc acggtggcgg cgggctttcc cactacggtg aatccgatcc
     5221 tgcagaacgg cgccgttccc gtcttcgtcg atgtatgcct gccaacctac aacatcgacg
     5281 tgacgatgct cgaagccgcc ctgtcgccgc ggaccaaggc catcatgatc gcccacacgc
     5341 tgggcaatcc gttcgacctc gatgcagtgt gcgcgttcgc cgagcagcat gggctatggc
     5401 tgatcgagga ctgctgcgat gcgctcggct ccacctggaa gggtcgcaag gtcggcacct
     5461 tcggccacct cgccacgctt tccttctacc cggcgcacca tatcaccatg ggcgaaggcg
     5521 gcgcggtgct caccaatgac ccgcagctgc ggcagatcgc cgaatccttt cgcgactggg
     5581 gccgcgactg ctggtgcaag cccggttgcg acaacacctg cggcaagcgc ttcggctggc
     5641 aacttggcag cctgccggcc ggctacgacc acaaatacac ctacagccac atcggctaca
//
LOCUS       DAOQNN010000201         5688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8274_length_5688_cov_11.362152, whole genome shotgun sequence.
ACCESSION   DAOQNN010000201 DAOQNN010000000
VERSION     DAOQNN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8274_length_5688_cov_11.362152"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            518..946
                     /gene="rplM"
                     /locus_tag="PKV97_10840"
     CDS             518..946
                     /gene="rplM"
                     /locus_tag="PKV97_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNB06416.1"
                     /translation="MKTFSAKPHEVKRDWFVVDGTDKVLGRLAAEVARRLRGKHKAIY
                     TPHVDTGDFIVVVNVEKLRVTGNKAQDKKYYRHSGYPGGIYETNFTKLQQRFPERVLE
                     KAVKGMLPKGPLGYAMLKKLKCYAGPSHPHTAQQPQVLEI"
     gene            958..1350
                     /gene="rpsI"
                     /locus_tag="PKV97_10845"
     CDS             958..1350
                     /gene="rpsI"
                     /locus_tag="PKV97_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNB06417.1"
                     /translation="MAVTYNYGTGRRKTAVARVFIKPGTGNIVVNGKPVDEFFSRETG
                     RMIVRQPLVLTGNEGRFDIMVNVVGGGESGQAGAVRHGITRALVDYDAGLKGDLRAAG
                     FVTRDAREVERKKVGLRKARRAKQFSKR"
     gene            1529..2566
                     /gene="argC"
                     /locus_tag="PKV97_10850"
     CDS             1529..2566
                     /gene="argC"
                     /locus_tag="PKV97_10850"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNB06418.1"
                     /translation="MVKVGIVGGTGYTGVELLRLLARHPGVELTAITSRGEAGMAVSD
                     MFPSLRRRVDLKFVTPQDAALEKCDAVFFATPNGIAMKQAAELVAAGVRVIDLAADFR
                     IRDVAEWQKWYGMEHAAPELVAEAVYGLPEVNRAQIRGARVLANPGCYPTAVQLGFLP
                     LVEAGLIDTDHLIADAKSGVSGAGRKAEVHTLLPEAADSFKAYGVPGHRHLPEIRQGL
                     ALAAGHAVGLTFVPHLTPMIRGIHATLYGRLKPGVDAADLQGLYEKRYAGEAFVDVMP
                     AGSHPETRSVRASNLCRIAVHRPQGGDTVVVLSVIDNLVKGAAGQAVQNLNIMFGFDE
                     ETGLDIVPVSP"
     gene            2657..3007
                     /gene="erpA"
                     /locus_tag="PKV97_10855"
     CDS             2657..3007
                     /gene="erpA"
                     /locus_tag="PKV97_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNB06419.1"
                     /translation="MSAEVATPEIMVFTDSAANKVRELIEEEGNPDLKLRVFVSGGGC
                     SGFQYGFTFDEEVNEDDTTFEKNGVLLLVDPMSYQYLVGAEIDYTEGLEGSQFVIRNP
                     NATSTCGCGSSFTV"
     gene            complement(3075..4304)
                     /locus_tag="PKV97_10860"
     CDS             complement(3075..4304)
                     /locus_tag="PKV97_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB06420.1"
                     /translation="MLGVVAATAVAPGSLPSDLLVERVVEALPAPAVTRTGTDASLPF
                     VHYERIQAGDTLQAMFNRLRIDDAEALAHLSASDAGRKALRQLRAGRSVTAVVSPQGR
                     LLSFSLPVGNTDAEVVLERDDAGLALRESSGATQTTMVEMRSGTITHSLFGATDDAGL
                     PDNVATQLATIFGTWIDFHTDLRKGDRFNVVYEAIYEEGNPVRAGRILAAEFINNGDR
                     HAVVLYRGPSGKEQYYSDDGESLQQGFLRSPLEFSRVSSNFGRRLHPIHGNWRDHNGT
                     DFSAPVGTPVMATSDATIEFIGTQRGFGNLIVLKHRSDITTHYAHLNGFAKGLAKGQA
                     VSQGDLIGYVGCTGWCTGPHLHYEVRLKDVPADPMTVALPMADSFNDRELAAFKRNTA
                     HLRQRFALLNYELADAR"
     gene            4489..5688
                     /gene="tyrS"
                     /locus_tag="PKV97_10865"
     CDS             4489..5688
                     /gene="tyrS"
                     /locus_tag="PKV97_10865"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNB06421.1"
                     /translation="MSDVQAAIDLIKRGADAVLIEAELVEKLKRGKPLRVKAGFDPTA
                     PDLHLGHTVLINKMRHFQELGHQVQFLIGDFTGMIGDPSGKNSTRPPLSREQILENAR
                     TYQEQVFKILDPEKTEICFNSAWMNDLGAAGMIRLAAQQTVARMLERDDFAKRYGNNQ
                     PIAIHEFLYPLCQGYDSVAMKADVELGGTDQRFNLLMGRELQKHYGQEPQCVVMVPLL
                     EGLDGVNKMSKSLGNYIGIAEPAKEIFGKTMSVSDTLMWRYYELLSFRSSAEIAQLRA
                     DVEGGRNPRDVKVMLAMEFVARFHGQRAAEEALADFEARFQRNAIPDDIPQVNVNVGA
                     GGLPLFQVLKQAGLTGTTSEAMRMVEQGGVRIDGERAEDKGLLLRAGETVVLQVGKRK
                     FASVVLS"
BASE COUNT          985 a         1787 c         1936 g          980 t
ORIGIN      
        1 ccttcgtgcc cgtgccgact tgcggttgaa acttccgggc ccgaggcgag cgcggccggg
       61 ttcaatacgg ctccgtagtg atgcactgca gcaaaacggg gcgcagggag gggggtgtcc
      121 gcgtccgggt gaggggcttg gaggtcgcga aaagagcgaa aattgattta aaataaaaag
      181 ctgctttgcg ataaagcgag ctgatcaatc aatcaaaact tttcttccgc gtcgcacaaa
      241 aatgccttgc acagcccggg gtggctatgc ataatccgtc ccagtcggat cgcagacgca
      301 tcgactccca cgtccggggt cgcgccgatc caagccgtgt ctcctccacc ctcctccttt
      361 ggtgtggatt taacgcagtc cgatcggact gcgttttttt tgttctccgc ccgcgttcgc
      421 gcttcgacga ttgacacgat tcatgtgttg gaagtaggat tgcgagcttt ccgtttgacg
      481 gccatctccg acggcccgac aaagatcgag gattcacatg aagacgtttt ccgccaagcc
      541 gcacgaggtc aagcgcgact ggttcgttgt cgacggcacg gacaaggtgc ttggccgtct
      601 cgccgccgaa gtcgcccgcc gcctgcgtgg caagcacaag gccatctaca ccccgcacgt
      661 ggacaccggc gacttcatcg tcgtggtcaa cgtcgaaaag ctgcgcgtga ccggcaacaa
      721 ggcgcaggac aagaagtact accgtcactc gggctacccg ggcggtatct acgaaaccaa
      781 cttcaccaag ctccagcagc gcttccccga gcgcgtgctc gagaaggcgg tgaagggcat
      841 gctgccgaag ggcccgctgg gctacgccat gctgaagaag ctgaagtgct acgcggggcc
      901 gtcgcatccg cacaccgctc agcagccgca agttctcgag atctgaagga gccgacaatg
      961 gctgtgactt acaactacgg taccggccgc cgcaagactg cggtcgctcg cgtgttcatc
     1021 aagccgggca ccggcaacat cgtcgtcaat ggcaagccgg tcgacgagtt cttctcgcgt
     1081 gaaaccggcc gcatgatcgt gcgccagccg ctggtcctca ccggcaacga aggccgtttc
     1141 gacatcatgg tcaacgtcgt cggcggtggc gaatccggtc aggcgggtgc ggtgcgtcac
     1201 ggcatcaccc gcgcgctggt cgattacgac gccggcctga agggcgacct gcgtgccgcc
     1261 ggtttcgtga cccgcgacgc ccgcgaggtc gagcgcaaga aggtcggcct gcgcaaggcc
     1321 cgtcgcgcca agcagttctc gaagcgctga ttctctttat cgcgcatcgc agtccggaag
     1381 ccgccccttg ggcggctttc gtgcttgtgt gtcccgctcg cacgcgttct tgcagttcgg
     1441 ggtgcggcag ccgtaacgag gcgttcaccg cccgcgtcgg ccgatgatga gataatcgcg
     1501 gttcagtctt tcgtcgcgag gaacgagcat ggtcaaggtt ggaatcgtag gcggtaccgg
     1561 atataccggc gtcgagctct tgcgtctgct ggcgcgccac cccggggtcg agctgaccgc
     1621 gatcacctcg cgcggcgagg ccggcatggc ggtctccgac atgttcccca gcctgcgccg
     1681 ccgggtcgat ctcaagttcg tcaccccgca ggacgccgcc ctggagaagt gcgacgcggt
     1741 gttcttcgcc acgcccaacg gcatcgcgat gaagcaggcg gccgagttgg tcgctgccgg
     1801 ggtgcgggtg atcgacctcg ccgccgactt ccgcatccgc gacgtcgccg agtggcagaa
     1861 gtggtacggc atggagcatg cggcgccgga actcgtcgcc gaggcggtgt atggcctgcc
     1921 cgaggtcaat cgcgcgcaga tccgcggcgc gcgcgtgctc gccaacccgg gctgctaccc
     1981 caccgcggtc cagctcggct tcctgccctt ggtcgaggcc gggctgatcg acaccgatca
     2041 cctgatcgcc gatgccaagt cgggcgtctc gggcgccggc cgcaaggccg aggtgcacac
     2101 cctgcttccg gaggccgccg actccttcaa ggcctatggc gtgccgggcc atcgccatct
     2161 gccggagatc cgtcaggggc tggctctggc cgccgggcac gcggtcgggc tgaccttcgt
     2221 gccccacctg acgccgatga tccgcggcat ccacgccacg ctgtacggtc gtctgaagcc
     2281 gggggtcgac gctgccgacc tgcaggggct gtacgagaag cgctatgctg gcgaggcctt
     2341 cgtcgacgtc atgcccgcgg gcagccatcc cgagacgcgc tcggtgcgcg cctccaacct
     2401 gtgccgcatc gcggtccatc gcccgcaggg cggcgacacc gtggtggtgt tgtcggtgat
     2461 cgacaatctg gtcaagggcg cggcaggcca ggcggtgcag aacctcaaca tcatgttcgg
     2521 tttcgacgag gaaaccgggc tcgacatcgt gccggtcagc ccctgaggga actgttacga
     2581 ccggattcga tcacattttg acgattcaac cgcggaaggc gcatcatccg cggtgttccc
     2641 cagcagagga gaaaacatga gcgcagaagt cgcaaccccg gaaatcatgg tctttaccga
     2701 tagcgccgcg aacaaggttc gcgagctgat cgaagaggaa ggcaatcccg acctcaagct
     2761 gcgcgtcttc gtgtcgggcg gcgggtgttc gggctttcag tacggcttca ctttcgacga
     2821 ggaagtgaac gaggacgaca ccacgttcga gaagaacggc gtgctgctgc tggtcgatcc
     2881 gatgagctac cagtacttgg tgggcgcgga gatcgactac accgaaggcc tggaaggctc
     2941 gcagttcgtg atccgcaatc cgaacgcgac cagcacctgc ggctgcggct cgtcgttcac
     3001 ggtgtgaacg cggttgtcgc aaacaaaacg gggcgccgcg gcgccccgtt tgctttggtc
     3061 cggccgtgcg gtgctcagcg tgcgtcggcg agttcgtagt tgagcagcgc gaagcgctgg
     3121 cgcagatgcg cggtgttgcg cttgaaggcg gcgagttccc ggtcattgaa ggagtcggcc
     3181 atcggcagcg ccaccgtcat cgggtcggcg gggacgtcct tcaggcgcac ctcgtagtgc
     3241 aggtgcggcc cggtgcacca gcctgtgcag ccgacgtagc cgatcagatc gccctggctc
     3301 accgcctgcc ccttggcgag gcccttggcg aagccgttga ggtgggcgta gtgggtggtg
     3361 atatcgctgc ggtgcttgag cacgatcagg ttgccgaagc cacgctgggt gccgatgaac
     3421 tcgatggtgg cgtccgaggt ggccatcacc ggggtgccga cgggtgccga gaagtcggtg
     3481 ccgttgtggt cgcgccagtt gccgtggatc ggatgcaggc ggcggccgaa gttcgagctg
     3541 acgcgcgaga actccagggg cgagcgcagg aagccctgct gcaggctctc gccgtcgtcg
     3601 ctgtagtatt gctccttgcc gctcggaccg cgatacagca ccaccgcgtg gcggtcgcca
     3661 ttgttgatga attcggccgc gaggatgcgc ccggcgcgca ccggattgcc ttcctcgtag
     3721 atggcctcgt agaccacgtt gaagcggtcg cccttgcgca ggtcggtgtg gaagtcgatc
     3781 caggtgccga agatggtggc gagctgggtc gccacgttgt cgggcaggcc ggcgtcgtcg
     3841 gtggcgccga aaagcgagtg ggtgatcgta cccgagcgca tctcgaccat cgtggtctgg
     3901 gtggctccgc tggattcacg cagcgcgagg ccggcgtcgt cgcgttcgag gacgacctcg
     3961 gcgtccgtgt tgccgaccgg cagcgagaag ctgagcagac ggccttgcgg cgacaccacc
     4021 gcggtgaccg agcggcccgc gcgcagctgg cgcagcgcct tgcggccggc atcggacgcg
     4081 gagaggtggg cgagcgcctc ggcgtcgtcg atgcgcaggc ggttgaacat cgcctgcagc
     4141 gtgtcgccgg cctggatgcg ttcgtaatgg acgaagggta gcgaggcgtc ggtgccggtg
     4201 cgcgtgaccg ccggcgcggg cagggcctcg acgacgcgct cgacgagcag gtccgaaggc
     4261 aggctgccgg gtgcgaccgc ggtcgcggcg accaccccga gcatggaaac cccggtgata
     4321 ccggcggcaa cccaggttcg cgggcgggag aggagacgag ccgggagatc ggctagaatt
     4381 cgggtctttc tggccttcat ggtctcggtg ggctgggatg tggaaaaaag tcgcggaagt
     4441 gtagcaaagg tgcgcttcgg ttcggcaaag taattttgga gaaattgaat gtctgatgtt
     4501 caggcggcga tcgacctcat caagcgcggc gccgacgccg tgctgatcga ggccgaattg
     4561 gtcgagaagc tcaagcgcgg caagcccctg cgggtgaagg ccggcttcga ccccaccgcg
     4621 cccgatctgc atctcggcca tacggtcctg atcaacaaga tgcgtcactt ccaggaactc
     4681 ggtcaccagg tccagttcct gatcggcgac ttcaccggca tgatcggcga ccccagcggc
     4741 aagaacagca cgcgcccgcc gctctcgcgc gagcagatcc tcgagaacgc caggacctac
     4801 caggagcagg tgttcaagat cctcgacccg gagaagaccg agatctgctt caactcggcc
     4861 tggatgaacg atctcggcgc cgccggcatg atccgcctgg ccgcgcagca gaccgtggcg
     4921 cgcatgctcg agcgcgacga cttcgccaag cgctacggca ataaccagcc gatcgcgatc
     4981 cacgagttcc tctacccgct gtgccagggc tacgactcgg tggcgatgaa ggccgacgtc
     5041 gaactcggcg gcaccgacca gcgcttcaac ctgctgatgg gccgcgagct gcagaagcat
     5101 tacgggcagg agccgcagtg cgtggtgatg gtgccgctgc tcgagggcct cgacggcgtc
     5161 aacaagatgt ccaagtcgct cggcaactac attggcatcg ccgagccggc caaggagatc
     5221 ttcggcaaga ccatgtcggt gtccgacacc ctgatgtggc gctactacga gctgctgtcc
     5281 ttccgctcca gcgccgagat cgcgcagctg cgcgccgacg tcgagggcgg gcgcaatccc
     5341 cgcgacgtca aggtgatgct ggcgatggag ttcgtcgccc gcttccacgg ccagcgtgcg
     5401 gccgaggagg cgcttgccga cttcgaggca cgcttccagc gcaacgcgat ccccgatgac
     5461 atcccgcagg tcaatgtgaa cgtgggcgcc ggcggcctgc cgctgttcca ggtgctcaag
     5521 caggccgggc tcaccggcac cacctccgag gcgatgcgca tggtggagca gggcggggtg
     5581 cgcatcgacg gcgagcgcgc cgaggacaag ggcctgctgc tgcgtgccgg cgagaccgtg
     5641 gtgctgcagg tgggcaagcg caagttcgcc tcggtggtgc tgtcttga
//
LOCUS       DAOQNN010000202         5665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8328_length_5665_cov_10.107130, whole genome shotgun sequence.
ACCESSION   DAOQNN010000202 DAOQNN010000000
VERSION     DAOQNN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8328_length_5665_cov_10.107130"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKV97_10870"
     CDS             complement(<1..778)
                     /locus_tag="PKV97_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNB06422.1"
                     /translation="MNRYPLWKNLLIGLVLIWGLIYTLPNFYGEVPAVQVSSAKATLK
                     LDTAATTDRIAGALKAAGIENTGIFSDATSVRARFKDTETQLRAKDVIEQTFNPDAAD
                     PSYVVALNLLSASPSWLTSIQALPMYLGLDLRGGVHFLLEVDMPGALVKRLDSTTGDL
                     RTLMRDKSVRHAGITREGNTVVIRFRDAAQREAGRRAILDSTTDLQLVERDDAADDLK
                     LVATLTPQAQQTMRDFAIKQNITTLHNRINELGVAEPVIQQ"
     gene            complement(939..1265)
                     /gene="yajC"
                     /locus_tag="PKV97_10875"
     CDS             complement(939..1265)
                     /gene="yajC"
                     /locus_tag="PKV97_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNB06423.1"
                     /translation="MLISNAYAQAAGAADPTGGLMGLLPLILMFVVLWFLMIRPQMKR
                     AKEHKAMVEALAKGDEVVTGGGIAGRVVEVGDSFVQIEVAPNTVVAAQKQAVATVLPK
                     GTLKAI"
     gene            complement(1357..2469)
                     /gene="tgt"
                     /locus_tag="PKV97_10880"
     CDS             complement(1357..2469)
                     /gene="tgt"
                     /locus_tag="PKV97_10880"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNB06424.1"
                     /translation="MQFELLTTDGGARRGRLTLAHGVVETPVFMPVGTYGTVKAMTPA
                     MLADIGAQICLGNTFHLWLRPGLDIISAHQGLHRFMAWDKPILTDSGGFQVFSLGALR
                     KISEEGVRFASPIDGAKLFLTPEISMQIQSVLNSDIVMIFDECTPYPATRDEAAKSMR
                     LSQRWAKRSRDEFDRLENKNALFGIVQGGMYEDLRDESQAALEDIGFHGFAIGGLSVG
                     EPKDDMARILAHTAPRLPQGKPRYLMGVGTPEDIVAGVAAGIDMFDCVMPTRNARNGW
                     LFTRYGDIKIKNAVHKADTRPLDPSCDCYTCRNFSRAYLHHLHRTGEILGSMLNTVHN
                     LRYYQTLTAELRAAIAAGEFAPYVQRFRGERATGTG"
     gene            complement(2459..3535)
                     /gene="queA"
                     /locus_tag="PKV97_10885"
     CDS             complement(2459..3535)
                     /gene="queA"
                     /locus_tag="PKV97_10885"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNB06425.1"
                     /translation="MSLTLADFDYTLPPELIAQAPLAERSASRLLVLEPQEGAAPRLT
                     DAVFSALAERVNPGDLLVFNDTRVIHARLHGVKDSGGQVEVLIERPIGPHEALAQVRA
                     SKSPKTGSRLRLADAFEAEVLGRVGEFFQLRFPPDEDLLALLERHGKLPLPPYIQRAA
                     GEADESRYQTVYARAPGSVAAPTAGLHFDDALLARIAARGARCAWLTLHVGAGTFQPV
                     RVDDLGEHRMHRERYVIPQETVDAIAATRAAGGRVIAIGTTSMRALEAAAQEGALEAG
                     SGETEIFILPGFRFQVVDALVTNFHLPRSTLMMLVSAFAGMDSIRRAYAHAVEQRYRF
                     FSYGDAMFITRRNESENDKNDDAV"
     gene            3632..3716
                     /locus_tag="PKV97_10890"
     tRNA            3632..3716
                     /locus_tag="PKV97_10890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3666..3668,aa:Leu,seq:caa)
     gene            complement(3943..4839)
                     /gene="nhaR"
                     /locus_tag="PKV97_10895"
     CDS             complement(3943..4839)
                     /gene="nhaR"
                     /locus_tag="PKV97_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HNB06426.1"
                     /translation="MLNYKQLHHFWTVARAGGIVRAAERSGLAPQTLSGQIATLETDL
                     GVNLFKRQGRGLVLTETGRMVLDYAEEIFRLGNELEEALQSRATGRAVPFRVGIADVV
                     PKAIAWLLLAPSMDLPEPMRIVCREGKFDALLGELAIHKLDVVLADSPLPPAMDVRGF
                     SHALGESTIGFYAAPALAARLAGDFPARLDGAPLLLPGVEASVRGPLLRWIEAAGLRP
                     RIVGEFDDSALMRAFAEAGAGIFPSASLVGEQLCRQGGLVHLGDAKGVTETYYAISVE
                     RRLTHPAVRAISEGAHAKQNFA"
     gene            4990..5322
                     /locus_tag="PKV97_10900"
     CDS             4990..5322
                     /locus_tag="PKV97_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNB06427.1"
                     /translation="MRIDLHCDGVETAPGLRDYVAQRMRASIGRFRDHIQWARVKVAD
                     VNGAEGGADKRCVVQLRLRNLPDVVFAITQLDVRAAVDEAADRVARVLAQRVRRHQRG
                     PVMAAIPA"
     gene            5419..>5665
                     /locus_tag="PKV97_10905"
     CDS             5419..>5665
                     /locus_tag="PKV97_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HNB06428.1"
                     /translation="MENRISTLGRAEASVLQTNKVIRNTYMLLSLTLAFSALTAGLSM
                     AMGAPRLGILITLAGYFGLLFATTKFRDSSLGIVFVFA"
BASE COUNT          826 a         1833 c         2068 g          938 t
ORIGIN      
        1 gctgctggat caccggctcg gcgacgccga gctcgttgat gcggttgtgc agggtggtga
       61 tgttctgctt gatcgcgaag tcgcgcatgg tctgctgcgc ctgcggcgtc agcgtggcga
      121 ccagcttgag gtcgtcggcg gcatcgtcgc gctcgacgag ctgcaggtcg gtggtgctgt
      181 cgaggatcgc gcgccggccc gcctcgcgct gcgcggcgtc gcggaagcgg atcaccacgg
      241 tgttgccttc gcgggtgatg ccggcgtggc gcacgctctt gtcgcgcatc agggtgcgca
      301 ggtcgccggt ggtcgaatcg aggcgcttga ccagcgcacc cggcatgtcg acctcgagca
      361 ggaagtgcac cccgccgcgc aggtcgaggc cgagatacat cggcagcgcc tggatcgagg
      421 tgagccagct cggcgaggcc gacagcaggt tgagcgcgac cacgtaggac gggtcggcgg
      481 cgtcggggtt gaaggtctgc tcgatgacgt ccttggcgcg cagctgggtc tcggtgtcct
      541 tgaagcgggc acgcacgctg gtggcgtcgg agaagatgcc ggtgttctcg atgccggccg
      601 ccttgagcgc accggcgatg cggtcggtgg tggccgcggt gtcgagcttg agcgtggcct
      661 tggcgctgga cacctgcacc gccggcacct cgccgtagaa gttgggcagg gtgtagatca
      721 ggccccagat cagcacgagg ccgatcagga ggttcttcca gaggggatag cggttcatcg
      781 tcggcgggaa tttgggtgga gcggggcccg acgggaggcg tcgggctgcg cctgccgggc
      841 gcctggccgc acccgtgggg gcggcgacgg attgcagggg ccggccatgg gcccgcggcg
      901 gcggcgcggt caagccgccc gccaccgcgc cgggagggtc agatcgcctt cagcgtgccc
      961 ttgggcagca cggtggcgac ggcctgtttc tgcgcggcga ccacggtgtt gggggcgact
     1021 tcgatctgca cgaagctgtc gccgacttcg acgacgcggc cggcgatgcc gccgccggtg
     1081 acgacttcgt cacccttggc gagcgcctcg accatggcct tgtgttcctt cgcgcgcttc
     1141 atctgcgggc ggatcatcag gaaccacagc accacgaaca tcaggatcag cggcagcagg
     1201 cccatcaggc ctccggtggg gtcggcggcg ccagcggcct gggcgtaggc attggaaatc
     1261 agcacgttga aactccggtt ggaagaaaaa cgatgaagta tagcagcagc cgggacgggg
     1321 cccgggaggg gtgagacggc ccgcgcggcg aagggttcag cccgtgccgg tggcgcgctc
     1381 gccgcggaag cgctgcacgt agggcgcgaa ctcgccggcc gcgatcgcgg cgcgcagctc
     1441 ggctgtaagc gtctggtagt agcgcaggtt gtgcaccgtg ttgagcatgc tgccgaggat
     1501 ctcgccggtg cggtgcaggt ggtgcaggta ggcgcggctg aagttgcggc aggtgtagca
     1561 gtcgcagctt gggtcgagcg ggcgggtgtc ggccttgtgc accgcgttct tgatcttgat
     1621 gtcgccgtag cgggtgaaca gccagccgtt gcgggcgttg cgggtgggca tcacgcagtc
     1681 gaacatgtcg atgccggcgg cgacgcccgc gacgatgtcc tcgggcgtgc ccacgcccat
     1741 caggtagcgc ggcttgccct gcggcaggcg cggcgcagtg tgggcgagga tgcgtgccat
     1801 gtcgtccttg ggttcgccca ccgacaggcc gccgatggcg aagccgtgga agccgatgtc
     1861 ttccagcgcc gcctgcgatt cgtcgcgcag gtcctcgtac atgccgccct ggacgatgcc
     1921 gaagagggcg ttcttgttct cgaggcgatc gaactcgtcg cgcgagcgct tcgcccagcg
     1981 ctgcgacagg cgcatcgact tggccgcctc gtcgcgagtg gcggggtagg gcgtgcattc
     2041 gtcgaagatc atcacgatgt ccgagttcag cacgctctgg atctgcatcg agatctcggg
     2101 cgtcaggaag agtttcgcgc cgtcgatcgg gctggcgaac ctcacgcctt cctcgctgat
     2161 cttgcgcagc gcgcccaggc tgaagacctg gaagccaccc gagtcggtga ggatgggctt
     2221 gtcccaggcc atgaagcgat gcaggccctg gtgcgcggag atgatgtcca gccccgggcg
     2281 cagccacagg tggaaggtgt tgcccaggca gatctgcgca ccgatgtcgg cgagcatggc
     2341 cggcgtcatc gccttgaccg tgccataggt gccgaccggc atgaagaccg gggtctcgac
     2401 tacgccgtgg gcgagggtga ggcgtccgcg gcgggcgccg ccgtcggtgg tgaggagttc
     2461 aaactgcatc gtcgttcttg tcgttctcgg attcgttgcg gcgggtgatg aacatcgcgt
     2521 cgccgtagct gaagaagcgg tagcgctgct cgaccgcatg cgcataggcg cggcggatac
     2581 tgtccatgcc ggcgaaggcc gacaccagca tcatcagggt ggatctgggc aggtggaagt
     2641 tggtgaccag cgcatccacc acctggaagc ggaagccggg caggatgaag atctcggtct
     2701 cgccgctgcc ggcttcgagc gcgccctcct gcgccgcggc ctcgagtgcg cgcatgctgg
     2761 tggtgccgat cgcgatcacg cgcccgccgg cggcgcgggt ggcggcgatg gcgtccaccg
     2821 tctcctgcgg gatcacgtag cgctcgcggt gcatgcggtg ctcgccgaga tcgtcgacgc
     2881 gcaccggctg gaaggtgccg gcgcccacgt gcagggtgag ccaggcgcac ctggcgccgc
     2941 gcgcggcgat gcgcgcgagc agcgcgtcgt cgaagtgcag gccggcggtg ggcgcggcga
     3001 ccgagccggg cgcgcgtgca tagaccgtct ggtaacggga ctcgtcggcc tcgcctgcgg
     3061 cgcgctggat gtagggcggc agcggcagct tgccgtggcg ctcgagcagc gcgaggaggt
     3121 cttcgtcggg cggaaagcgc agctggaaga actcgcccac gcggccgagc acctcggcct
     3181 cgaacgcgtc cgccaggcgc agccggctgc cggtcttcgg cgacttgctc gcccgcacct
     3241 gggcgagcgc ctcgtgcggg ccgatcggcc gctcgatcag cacctcgacc tggccgccgc
     3301 tgtccttgac gccgtgcaga cgggcatgga tgacgcgggt gtcgttgaac accagcagat
     3361 cgccgggatt cacgcgctcc gcaagggcag aaaacaccgc gtcggtgagc cgcggcgcgg
     3421 cgccctcctg cggctccagc acgagcaggc ggctggcgct gcgctcggcc agcggtgcct
     3481 gggcgatcag ctcgggaggc agggtgtagt cgaagtcggc gagggtgagg gacatcgtgt
     3541 cgtgttcggt ggagattgtt ctggtccgga gcgtgcggtt gacggctcgc ttgccgcccc
     3601 gcctcgtgtt cggggataat gcgcgtcccg tgccgggatg gcggaatcgg tagacgcagc
     3661 ggattcaaaa tccgccgccg caaggtgtgt gggttcgagt cccactctcg gcaccagttt
     3721 tcggctccgc cggtcgtgca tcgaccgccg gcggctcttc gatgaatgcg gcgttcgacc
     3781 ccgcccgtcg agtagtcgtt cgcgacggag tcctccgctc gcttttcctc ttcctgcgtt
     3841 ccggctgcct gccttcttgc tgagggagcg cccttgccag cgttgccggt cgccgtccgt
     3901 ttccggcccg cgattctgta gcgctccggg gcgaaaatca aatcaggcga agttctgctt
     3961 cgcgtgcgcg ccctcgctga tcgcgcgcac cgcggggtgg gtcagtctgc gctccaccga
     4021 gatcgcgtag taggtttcgg tgacgccctt cgcgtccccc agatgcacca gcccgccctg
     4081 gcggcagagc tgctcgccga ccagcgaagc gctcgggaag atgcccgcgc cggcctcggc
     4141 gaaggcgcgc atcagcgcgc tgtcgtcgaa ttcgccgacg atgcgcgggc gcagcccggc
     4201 ggcctcgatc cagcgcagca gcggcccgcg cacgctcgcc tccaccccgg gcagcagcag
     4261 cggtgcgccg tcgaggcggg cggggaagtc gccggcaagg cgggcggcga gcgcgggcgc
     4321 ggcgtagaag ccgatggtcg attcgccgag cgcgtggctg aaaccgcgca cgtccatggc
     4381 gggcggcagc gggctgtcgg cgagcaccac gtcgagcttg tggatcgcga gttcgccgag
     4441 cagggcgtcg aacttgccct cccggcagac gatgcgcatc ggctcgggga gatccatcga
     4501 gggtgccagc agcaaccacg cgatcgcctt cggcaccacg tcggcgatgc ccacccggaa
     4561 aggcaccgcg cgccccgtcg cgcggctctg cagcgcttcc tcgagctcgt tgccgaggcg
     4621 gaagatctcc tcggcgtagt ccagcaccat gcgcccggtc tcggtcagca ccaggccgcg
     4681 gccctggcgc ttgaacaggt ttacgccgag atcggtctcc agggtggcga tctggccgga
     4741 cagggtctgc ggcgccaggc cggaacgttc cgccgcgcgc acgatcccac ccgcgcgggc
     4801 cacggtccag aaatgatgga gctgcttgta gttcagcatg gggcgcctcc ggcgtgcgca
     4861 cttcgatact tcggaaaaat cggattcaaa ttctgctgaa ccgagattct atcgaacagt
     4921 ctgagcggta gtatccaccg gagcacaggg cccgatggat ccgtgcaacg aaaacgaagg
     4981 agactggaca tgcgtatcga tctgcattgc gacggtgtgg agaccgcccc cggcctgcgc
     5041 gactacgtgg cgcagcggat gcgtgcttcg attggccgct tccgcgacca catccagtgg
     5101 gcgcgcgtga aggtcgccga tgtgaatggt gcggagggcg gcgccgacaa gcgttgtgtc
     5161 gtgcaactgc gcctgcgcaa cctccccgat gtggtcttcg ccatcaccca gctcgacgtg
     5221 cgcgcggcgg tggacgaggc ggccgaccgg gttgcccgcg tgctcgccca gcgcgtgcgc
     5281 cgccaccagc gcgggccggt gatggccgcg atcccggcat gattttctcg tagttcctgc
     5341 agcaaacccc ccccggtcgt ccgcccgttc ggcgccggtt ccaaaacaaa tcgacgatcc
     5401 cactggagga cctccaccat ggaaaaccgc atcagtaccc tgggccgggc cgaagcctcg
     5461 gtcctgcaga ccaacaaggt catccgcaac acctacatgc tgctgtcgct cacgctggcc
     5521 ttctcggcgc tgaccgccgg cctgtcgatg gcgatgggcg cgccgcgcct gggcatcctg
     5581 atcacgctgg ccggctactt cggcctgctc ttcgccacca ccaagttccg cgacagcagc
     5641 ctgggcatcg tgttcgtgtt cgcgc
//
LOCUS       DAOQNN010000203         5663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8334_length_5663_cov_8.993937, whole genome shotgun sequence.
ACCESSION   DAOQNN010000203 DAOQNN010000000
VERSION     DAOQNN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8334_length_5663_cov_8.993937"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKV97_10910"
     CDS             complement(<1..398)
                     /locus_tag="PKV97_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNB06429.1"
                     /translation="MTALRFEGAQDYVATPDLMLAVNAAIRLQRPLLIKGEPGTGKTM
                     LAEQVAAALGLPLLQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDDRVKDIANYIVKGV
                     LWQAFEAEQPTVVLIDEIDKADIEFPNDLLR"
     gene            515..1558
                     /locus_tag="PKV97_10915"
     CDS             515..1558
                     /locus_tag="PKV97_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB06430.1"
                     /translation="MEIEQLRLRLRALGARPSHEARLLRAWLQGHALDAGPRTESNRL
                     PKALQAALPELEAELGALARVRSEHPGEDGTRLLVELADGQAVESVLLPRDGLCVSTQ
                     VGCAVGCVFCMTGKDGLLRQLDSGEIVAQVALARRLRRVHKVVFMGMGEPAHNLDAVI
                     EAIELLGTEGALPHKNLVLSTVGDLRVFERLPQMRVKPALALSLHSTRAALRAALLPR
                     APRIDPAELVELGEAWARASGYPIQYQWTLIEGVNDGEDELEGIVRLLAGKYAVMNMI
                     PFNRVDGLDYRRPPPERAAEIARRLHRAGILTKLRQSAGQDVDGGCGQLRAREAKLAF
                     VRREERRAARPPA"
     gene            complement(1555..2031)
                     /locus_tag="PKV97_10920"
     CDS             complement(1555..2031)
                     /locus_tag="PKV97_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HNB06431.1"
                     /translation="MNHTLRSPLFRKLGRCLSALACGAALGLAAPALASPDEVVMYKD
                     PNCGCCGKWAEHMRSAGFAVKEVANPRMDLVKQEAGVPEALGSCHTAKVAGYVVEGHV
                     PAADVRRMLTEKPAIRGLSAPGMPLGSPGMEGPYPAERYDVVSFDAQGRSAVFSKH"
     gene            complement(2028..3182)
                     /locus_tag="PKV97_10925"
     CDS             complement(2028..3182)
                     /locus_tag="PKV97_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06432.1"
                     /translation="MSTLPPLARVPLLVLGLLSLLTGVFAGLARLGVEVPALAAVHAG
                     NHAALMICAFFGTVISLERAVALGGLWPYAGPAAAGTGGVLLLAGGPLVAAQLLFLLA
                     AATLFGGGVVVVRRQLALFTVMLAVGAACWLLGSLVWWNTGNVHAAVPLWLAFLVLTI
                     AGERLELTRFLPARPAAAPSFVAVSALILAGAVLAPMHEDFGHGLFAAGVLAMAAWLL
                     TFDIARHNARQQGLTRFIAICLLSGYVWLAFGALAALGGGLAPVSPLHDITMHAIALG
                     FVFSMVFGHAAIIFPAVLKVKIPYHPAFYMPLALLHASLALRVAGSLLELPALRSWGG
                     IANALTLAVFIATMVVSVIRGGRPPARRRRNPVPGTPVRSSVKPPGNPAP"
     gene            complement(3179..3499)
                     /locus_tag="PKV97_10930"
     CDS             complement(3179..3499)
                     /locus_tag="PKV97_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNB06433.1"
                     /translation="MSTPPTPDAEEIRHALRQVMDPEVGMNIVDLGLIYRIDCAPGEL
                     YIEMTMTTPACPLSDMILDNIDAVLDPLLPPEVDIRVELVWDPPWSPAMMEEGARAHF
                     GWNG"
     gene            complement(3627..>5663)
                     /locus_tag="PKV97_10935"
     CDS             complement(3627..>5663)
                     /locus_tag="PKV97_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HNB06434.1"
                     /translation="LHPSPADLAALRAGNPAYLQQTASAARSNPNALFAITLAAFVQP
                     GSVDLAPLHARLVASRFSDRGSGQVEPLALAYDWLHDQWSAQERESLRERLAEGCDFL
                     IEVIRKEQLSPYNAFLYNTPLQGLMACSIALYGDHPRGEAFMRFTHELWKKRVLPVWR
                     QVFGRHGGWHEGGEYVAVGIGQAIHTLPALWRTATGEDLFASEAGIRGFLDFLVYRTR
                     PDRTHMRWGDGAWFDRHPRDAAALALEYRHAAAYTLAPPNAARTRDGRRVGPVPTGWP
                     WGPLSDDGLIDPAAQTRMPLARLFDGIGLLVARSDWSEDATWLSFKAGDNFWSHSHLD
                     QGAFTIFKGGPLAIDSGWYGPAYGSNHHMNYTYQSIAHNLVTVTDPADEQPGPGFDAA
                     NPRHYPNDGGQRRIGSGWGVDAAPLDVAQWQERSETYHTGRIAAHLDDDDLVVAVADV
                     GAAYTNRNSGRGSFADRTRRVERMWRVLGYDRINDAVVVFDDVVASRAGFAKRWLLHA
                     VEPPLVRGDRFDLFIPGDTRPGRRGGSLHGYVLLPRDAVLDTVGGPGFEFFVDGRNHD
                     EDGKVQAAIAKLGHGRAEPGAWRIELRPRAAAAEDRFLVVMLPTLAGDQPQARVRLLE
                     AGAEVGAEIAGPRRTTRWWFVPGRLGARVEVLEDGRTRSHEIVPGGSPAGNITD"
BASE COUNT          812 a         1919 c         2148 g          784 t
ORIGIN      
        1 cgcagcaggt cgttggggaa ttcgatgtcg gccttgtcga tctcgtcgat cagcaccacg
       61 gtcggctgct cggcctcgaa ggcctgccac agcacgccct tgacgatgta gttggcgatg
      121 tccttgacgc ggtcgtcgcc gagctgcgag tcgcgcaggc gggagaccgc gtcgtactcg
      181 tagaggccct gctgcgcctt ggtggtcgac ttgatgtgcc actgcagcag cggcagtccg
      241 agcgcggcgg cgacctgctc ggcgagcatg gtcttgccgg tgccgggctc gcccttgatc
      301 agcagcgggc gctgcaggcg gatcgcggcg ttgaccgcga gcatcaggtc gggggtggcg
      361 acgtagtcct gggcgccttc gaagcgtagt gcggtcatgc ggcattcctc gtgtcggatg
      421 cgggcgatta tagggccggg catggcaggc gggcagccgg accggtgtcg atcgcggatg
      481 ctctcgccct tttcgggtgc tagcatcgcg cgccatggaa atcgaacagc tccgcctgcg
      541 cctgcgcgcg ctcggcgccc gccccagcca cgaggcccgc ctgctgcgcg cctggctgca
      601 gggacacgcc ctcgacgccg gtccgcgcac cgaatccaac cgcctgccca aggccttgca
      661 ggccgcgctg cccgagctcg aagcggagct cggagcgctg gcccgcgtgc gctccgagca
      721 ccccggcgag gatggcaccc gcctgctggt cgagctcgcc gacggccagg cggtggagag
      781 cgtgctgctg ccgcgcgacg gcctgtgcgt gtccacccag gtcggctgcg cggtgggctg
      841 cgtgttctgc atgaccggca aggacggcct gctgcgccag ctcgacagcg gcgaaatcgt
      901 cgcccaggtc gcactcgcgc gccgcctgcg ccgggtgcac aaggtggtgt tcatgggcat
      961 gggcgagccc gcgcacaacc tcgatgcggt gatcgaggcg atcgagctgc tcggcaccga
     1021 gggcgcgctg ccgcacaaga acctggtgct gtcgaccgtg ggcgacctcc gcgtgttcga
     1081 gcgtctgccg cagatgcggg tcaagccggc gctggcgctc tcgctgcaca gcacgcgcgc
     1141 cgcgctgcgc gccgcgctgc tgccgcgcgc cccgcgcatc gacccggccg agctggtcga
     1201 gctcggcgag gcctgggcgc gcgcgagcgg ctatccgatc cagtaccaat ggacgctgat
     1261 cgagggcgtg aatgacggcg aggacgagct cgagggcatc gtgcgcctgc tcgccggcaa
     1321 gtacgcggtg atgaacatga tcccgttcaa ccgcgtcgac gggctcgatt accgccgccc
     1381 gccgcccgag cgcgcggcgg agatcgcccg ccgcctgcac cgcgccggca tcctcaccaa
     1441 gctgaggcag tccgccgggc aggacgtgga cggcggctgc gggcagctgc gcgcccgcga
     1501 ggccaagctc gccttcgtgc gtcgcgaaga gcggcgcgcg gcgcggccgc ctgcctagtg
     1561 cttcgagaac actgcgctcc gcccctgcgc gtcgaagctc accacgtcgt agcgttcggc
     1621 cgggtaggga ccttccatgc cgggcgagcc tagcggcatg ccgggcgcgg ataggccgcg
     1681 gatcgccggc ttctcggtca gcatgcggcg cacgtcggcc gccgggacgt ggccctcgac
     1741 gacatagccg gccaccttcg cggtgtggca ggagcccagg gcctcgggca cgccggcttc
     1801 ctgcttgacg agatccatgc gcgggttggc gacctccttc acggcgaagc cggcactgcg
     1861 catgtgctcg gcccatttgc cgcagcagcc gcagttggga tccttgtaca tcacgacttc
     1921 gtcggggctg gcgagggcgg gcgcggcgag gccaagcgcg gcgccgcagg cgagcgcgga
     1981 caggcagcga ccgagcttgc ggaagagggg ggagcggagg gtgtggttca tggtgcgggg
     2041 ttcccgggag gtttgacaga ggatcggacc ggtgtgcccg ggacggggtt ccggcgccgg
     2101 cgcgccggcg ggcgtccgcc gcggatcacg ctgacgacca tggtggcgat gaacaccgcg
     2161 agggtcagcg cattcgcgat cccgccccaa ctgcgcaacg cgggaagctc gagcaggctg
     2221 cccgccacgc gcagcgcgag cgaggcgtgc agcagcgcga gcggcatgta gaaggccggg
     2281 tggtagggga tcttcacctt cagcacagcc gggaagatga tcgcggcgtg gccgaacacc
     2341 atcgagaaaa cgaagccgag cgcgattgca tgcatggtga tgtcatgcag tggggacacg
     2401 ggtgcgagcc cgccgccgag cgcggcgagc gcaccgaagg ccagccagac atagcccgac
     2461 agcaggcaga tcgcgatgaa gcgggtcagc ccctgctggc gggcgttgtg gcgggcgatg
     2521 tcgaacgtca gcagccaggc cgccatcgcg agcacgccgg cggcgaagag gccgtggccg
     2581 aagtcttcgt gcatcggcgc gagcacggcg ccggcgagga tcagcgcgct gaccgcgaca
     2641 aagctcggcg ccgcggccgg gcgtgcgggc aggaagcggg tgagctcgag gcgttcgccc
     2701 gcgatcgtca gcaccaggaa ggcgagccac agcggcaccg ccgcgtgcac gttgccggtg
     2761 ttccaccaca ccaggctgcc cagcagccag catgccgcgc ccaccgcgag catcacggtg
     2821 aatagcgcca gctgccggcg caccaccacc acgccgccac cgaacagggt cgccgcggca
     2881 agcaggaaaa ggagctgcgc ggcgaccagc ggaccacccg cgagcagcag cacgccaccc
     2941 gtcccggcgg cggccggccc ggcgtagggc cacaggccgc cgagcgcgac cgcgcgctcg
     3001 aggctgatca cggtgccgaa gaaggcgcag atcatcagcg ccgcatgatt gcccgcatgc
     3061 acggcggcaa gcgccggcac ctccacgccc aggcgggcga ggccggcgaa aacgccggtg
     3121 agcagcgaca gcagcccgag caccagcagg gggacacggg cgagcggggg cagggtgctc
     3181 atccgttcca gccgaagtgc gcgcgtgcgc cctcttccat catcgccggt gaccagggcg
     3241 gatcccacac cagctcgacg cggatgtcca cctcgggtgg caacaggggg tcgagcaccg
     3301 cgtcgatgtt gtcgaggatc atgtcggaga gcgggcaggc cggcgtggtc atcgtcatct
     3361 cgatgtagag ctcgccgggt gcgcagtcga tgcggtagat cagcccgagg tcgacgatgt
     3421 tcatgccgac ctccggatcc atgacctggc gcagtgcgtg gcggatctct tcggcgtcgg
     3481 gagtgggcgg tgtgctcatg tgggggcgtc cagggtgcgg gccgcgaccg tcgccgggac
     3541 ggctgcaagc gggtttgcgg cggtcggcgg gcgctgtgca gcccgccgcg gtgcgtgggg
     3601 cattgtcggc caggtcggcg aatccttcaa tccgtgatgt ttccggcggg ggatccgccg
     3661 ggcacgatct cgtggctgcg cgtgcgaccg tcctcgagca cctccacgcg tgcgcccagg
     3721 cgtccgggca cgaaccacca gcgtgtggtg cgccgcggcc cggcgatctc cgcacccacc
     3781 tccgcgccgg cttcgagcag gcgcacgcgg gcctggggct ggtcgccggc gagcgtgggc
     3841 agcatcacga cgaggaagcg gtcttcggcg gccgcggcgc gcggccgcag ctcgatgcgc
     3901 caggcgcccg gctcggcgcg gccgtggccg agcttcgcga tcgccgcctg caccttcccg
     3961 tcctcgtcgt ggttgcgccc gtccacgaag aactcgaagc cggggccgcc tacggtgtcg
     4021 agcaccgcgt cgcgcggcag gaggacgtag ccgtgcaggc tgccgccccg ccgccccggg
     4081 cgggtgtcgc ccgggatgaa cagatcgaag cggtccccgc gcacgagcgg cggctccacc
     4141 gcatgcagca gccaacgctt ggcgaagccg gcacggctgg cgacgacgtc gtcgaacacc
     4201 accaccgcat cgttgatccg gtcatagccc agcacccgcc acatgcgctc gacgcggcgg
     4261 gtgcggtcgg cgaagctgcc gcgtccggag ttgcggttgg tgtaggcggc gccgacgtcg
     4321 gccacggcga cgaccaggtc gtcgtcgtcg aggtgggccg cgatgcggcc ggtgtggtag
     4381 gtctcgctgc gctcctgcca ttgtgcgacg tcgagcggcg ccgcatccac gccccagccc
     4441 gagccgatgc ggcgctggcc gccgtcgttg gggtagtggc gcgggttggc ggcgtcgaag
     4501 ccggggccgg gctgctcgtc ggcggggtcg gtcaccgtca ccaggttgtg ggcgatgctc
     4561 tggtaggtgt agttcatgtg gtgattcgac ccgtaggcgg gaccgtacca gccgctgtcg
     4621 atcgccagcg gccccccctt gaagatcgtg aacgcgccct ggtcgaggtg gctgtgcgac
     4681 cagaagttgt cgccggcctt gaaactgagc caggtggcgt cctcagacca gtcgctgcgc
     4741 gccacgagca ggccgatgcc gtcgaacagg cgcgcgagcg gcatgcgggt ctgcgcggcg
     4801 gggtcgatca ggccgtcgtc cgacagcggt ccccatggcc acccggtggg caccgggccg
     4861 acccgacgcc cgtcgcgcgt gcgcgccgcg ttgggcggtg ccagcgtgta ggcggcggcg
     4921 tggcggtact cgagggcgag cgccgcggcg tcgcgcggat ggcggtcgaa ccaggcgccg
     4981 tcgccccagc gcatatgggt gcgatcgggg cgggtgcgat agacgaggaa gtcgaggaag
     5041 ccgcggatcc ccgcttcgct ggcgaacagg tcctcgccgg tggcggtgcg ccacagcgcg
     5101 ggcagggtat ggatggcctg gccgatgccc accgccacgt attcgccgcc ttcgtgccag
     5161 ccgccgtggc gcccgaagac ctggcgccag accggcagca cacgcttctt ccacagctcg
     5221 tgggtgaagc gcatgaaggc ctcgccgcgc ggatggtcgc cgtacagcgc gatgctgcac
     5281 gccatcaggc cttgcagcgg ggtgttgtag aggaaggcgt tgtagggcga gagctgttcc
     5341 ttgcggatca cctcgatcag gaagtcgcag ccctcggcga ggcgctcgcg caggctctcg
     5401 cgttcctgcg cgctccattg gtcgtgcagc cagtcgtagg cgagcgcgag cggttcgacc
     5461 tggccgctgc cgcggtcgct gaagcgactc gccaccaggc gcgcgtgcag cggggcgaga
     5521 tcgacgctgc cgggctggac gaaggccgcc agcgtgatcg cgaagagggc gttcgggttg
     5581 ctgcgcgccg cgctcgccgt ctgctgcagg taggcggggt tgccggcgcg cagcgccgcg
     5641 aggtcggcgg gggagggatg gag
//
LOCUS       DAOQNN010000204         5624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8431_length_5624_cov_5.792781, whole genome shotgun sequence.
ACCESSION   DAOQNN010000204 DAOQNN010000000
VERSION     DAOQNN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8431_length_5624_cov_5.792781"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1387
                     /locus_tag="PKV97_10940"
     CDS             110..1387
                     /locus_tag="PKV97_10940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB06435.1"
                     /translation="MPIEDPTLRNARDAELVTYANDIAAAIRPTAARYDRTGEFPFEH
                     FEILRHNGALGLTVPEEYGGRGLSLYETLLVQERIAGASGATALALGWHLMTFFGLSA
                     TRAWDEAVFERVCRDAVERGELINVFATERDAGNVVRGFKPTTVARRHGDGYVISGRK
                     AFSTLAPAVNHFTVLAHIEEDDQTAEFLVRKSARVRIVETWDSLGMRSTGSHDVELDD
                     VEVPADALLVRLEKGKPSRFMACSSAYSLQLPAIYLGIAIAARDFILDFADSRFAPSL
                     GSVILDAPQVQHKIGEIEILIGVSKSLLYGLAERWDRHDDLKDKLGSEVAITKYAVTN
                     NAIRIVELAMQIAGGHALSRDLPLERHFRDVQCGLYNPPHNDMVIDRVAKEAIARQRA
                     DRTPSAVIPITPTPQGPDRTAHTATTRSSEAIA"
     gene            1444..2829
                     /locus_tag="PKV97_10945"
     CDS             1444..2829
                     /locus_tag="PKV97_10945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNB06436.1"
                     /translation="MPALAFNAFVMNTASHIQHGQWRHPDARQVDFNDVDLWVDLART
                     LERGLFDAIFFADVVGLYGPLGGDYEVNAREGLQIPSNDPSVLLSALAVNTEHLGLAF
                     TSSVLQAHPFDFARKVSTLDHISRGRVAWNIVTTHLENAARNFGQDQLTPHDERYAWA
                     AEYVDVVYKLWEGSWDEGALLIDRARGVFADHNKIHKIHHVGPRYRVEGPHLSAPSPQ
                     RTPLLFQAGSSGAGRQFAAANAEGQFIVTPNPTVAAELIRDTRRLAVATGRRAEDIKF
                     FQGLSFIIGSTEAEVRHKEAEYDAYASVDGFLAHHNYAVDRYGRPYPADTPLAAIQSE
                     ASQSILDWYAKSSPGTEPTVADLGRLASRRGRVAGTPEQIADQLELWQQAGVDGINVI
                     NWHLPGSYEEFNEHLLPVLQQRGLAKREYRPGTLRQKLFGSDRINERHPAARYRGAFT
                     PKTETRNVEFA"
     gene            2826..4145
                     /locus_tag="PKV97_10950"
     CDS             2826..4145
                     /locus_tag="PKV97_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB06437.1"
                     /translation="MNARQSGLPHPFIRTKSGAEPAVSPTAVAPALAAPAPQFETLAA
                     RFRPIFARIAERAAEREHDRELPFDAVGWLREARFGALRVPVEHGGFGASVEQFFALL
                     IELAEADSNLPNIFRAHFGQIERLYAEIDPALHAPWLERAAAGEIFGNATTEIGDGAL
                     GQPATRITKQEGVLRLNGTKYYSSGSLYADWIAVSAQLQEEGVAGGAEQPVIALVRAD
                     APGVERIDDWKGFGQRLTGSGTTRLTDVEGDPASIWPCARSGPTSMTALFQLVHLATL
                     TGIARAIERDACAYVRPRKRVFSHGSGAIPREDPLIQQVVGQLASTAFIAEAAVRAVA
                     DRLGDIDRLRRRGEAVPESLLVDVELAAAKAQVGLVDAVLTSTTRLFDVGGASAVQEN
                     RRLDRHWRNARTLASHNPVIFKARVVGDHAINGTAPGFYWTVGAATG"
     gene            complement(4196..>5624)
                     /locus_tag="PKV97_10955"
     CDS             complement(4196..>5624)
                     /locus_tag="PKV97_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNB06438.1"
                     /translation="IKKAEDDPNVERKKAAKALARFMRLHPHNIGQKTEVMVEHFQHF
                     TRHKIGGHAKAMVVTGSRLEAVRYKQEFDRYIQEKGYPIKSLVAFSGSVEDDRIAEKT
                     YTEVEMNGGLKEKALPETFAKPDFRVLLVAEKYQTGFDQPLLHTMYVDKRLAGIQAVQ
                     TLSRLNRTHPLKDDTFVLDFVNEPGEIQEAFRQYYEGAVMGEEVDPDKLYAVKAELDA
                     SGIYRQTEVSDFSRVFFAPKRRQSPGDHKAMNAILDQAVARFVQLQNTEEEEAELWRG
                     KLQAFRNLYGFLSQVIPYQDSDLEKLFTYLRHLALKLPKRKSGPGYQFDEEVELDYYR
                     LQKISEGSISLDEGYAKPLDGPREVGSGMVREEHVSLSRLIDIINQRFGGELNEADQL
                     FFDQIAEAASLNESLQRAAEVNSLDKFQLVFRQVLESLFIERMELNEELFTDYMAKPE
                     MQALVSKWLGGQVYDRLSAARRRVS"
BASE COUNT          933 a         2060 c         1746 g          885 t
ORIGIN      
        1 ccctgcacga cccggtggat tacaaggtgc gcgaactcgg cgactgggcg ctcaacggcc
       61 actacccgaa gccttccttc tattcctgag cagccgcgga gcaaagccca tgcccatcga
      121 agatccgacc ctgcgcaacg cgcgcgacgc cgaactcgtc acctacgcca atgacattgc
      181 tgccgcgatc cggccgacgg cagcgcgcta cgaccggacg ggcgaattcc cgttcgagca
      241 cttcgagatc ctgaggcaca acggcgcgct cggcctcacc gtccccgagg aatacggcgg
      301 ccgggggctg tcgctgtacg agacgctgct ggtgcaggag cgcatcgccg gcgccagcgg
      361 cgcgaccgcg ctcgcgctcg gctggcacct gatgaccttc ttcggcctca gcgcgacgcg
      421 cgcctgggac gaggccgtgt tcgaacgcgt gtgccgcgat gccgtcgagc gcggcgagct
      481 gatcaacgtc ttcgccaccg aacgcgatgc cggcaacgtc gtgcgcggct tcaagcccac
      541 caccgtcgcc cgccgccacg gcgacggcta cgtcatctcg gggcgcaagg ccttctccac
      601 cctggcgccc gcagtgaacc acttcaccgt gctcgcccat atcgaggaag acgaccagac
      661 ggccgagttc ctggtgcgca agtccgcacg cgtgcgcatc gtcgaaacct gggattcgct
      721 gggcatgcgc agcaccggca gtcacgacgt cgagctcgac gacgtggaag tgcccgcgga
      781 cgccctgctg gtccgcctcg agaagggcaa gccgagccga ttcatggcct gcagcagcgc
      841 ctactcgctg cagttgccgg cgatctatct cggtatcgcc atcgccgcgc gcgacttcat
      901 cctcgacttc gccgacagcc gcttcgcgcc cagcctcggc agcgtgatcc tggacgcgcc
      961 gcaggtgcag cacaagatcg gcgagatcga gatcctgatc ggcgtgtcga aatcgctgct
     1021 gtacggcctg gccgagcgct gggaccgcca cgacgacctc aaggacaagc tcggcagcga
     1081 ggtcgcgatc accaagtacg cggtgaccaa caacgccatc cgcatcgtcg aactcgcgat
     1141 gcagatcgcc ggcggccacg cgctgtcgcg cgacctgccg ctggagcgcc acttccgcga
     1201 cgtgcaatgc ggcctctaca acccgccgca caacgacatg gtcatcgatc gcgtcgccaa
     1261 ggaggcgatc gcgcgtcagc gcgcagaccg caccccgagc gccgtcatcc cgatcacgcc
     1321 caccccgcaa ggcccagacc ggaccgccca cacagccacg acacgcagca gcgaagccat
     1381 cgcttgaacg acgcccgccc gttgccgcct ctccgccctc catccctcgg aacaggacac
     1441 cccatgcccg ccctcgcctt caacgccttc gtcatgaaca ccgcctccca catccagcac
     1501 ggacagtggc ggcaccccga tgcccgacag gtcgatttca acgacgtcga tctgtgggtc
     1561 gacctcgcgc gcacgctcga gcgcggcctg ttcgacgcca tcttcttcgc cgacgtggtc
     1621 ggcctctatg gtccgctcgg cggcgactac gaggtcaatg cccgggaggg gctgcagatc
     1681 ccgagcaacg acccgtcggt gctgctgtcg gccctggcgg tgaacaccga gcacctcggg
     1741 ctcgccttca ccagttcggt gctgcaggcc caccccttcg acttcgcgcg caaggtatcg
     1801 acgctggatc acatctcgcg cggacgggtg gcgtggaaca tcgtcaccac ccacctggag
     1861 aacgccgcgc gcaacttcgg ccaggaccag ctcaccccgc acgacgagcg ctacgcctgg
     1921 gcggcggaat acgtcgatgt cgtctacaag ctgtgggaag gctcgtggga cgagggcgcg
     1981 ctgctgatcg accgcgcgcg cggcgtgttc gcagaccaca acaagatcca caagatccac
     2041 cacgtcggcc cacgttaccg ggtggaaggg ccgcacctga gcgcgccctc gccgcagcgc
     2101 acgccgctgc tgttccaggc cggctcatcg ggcgccggac gccagtttgc cgcggccaat
     2161 gccgaaggcc agttcatcgt cacccccaat cccacggtgg ctgcggagct gatccgcgac
     2221 acccgccgcc tcgcggtggc caccggccgc cgcgccgaag acatcaagtt cttccagggc
     2281 ctgagcttca tcatcggcag caccgaggcc gaagtgcggc acaaggaagc ggaatacgac
     2341 gcatacgcca gcgtcgacgg cttcctcgcc caccacaact acgcggtcga ccgctacggc
     2401 aggccctacc cggccgacac cccgctggcg gcgatccagt ccgaggccag ccagagcatc
     2461 ctcgactggt acgcgaagag ctcgccgggc accgaaccga ccgtggccga cctcggccgc
     2521 ctggcctcgc gccgcggccg tgtcgccggc acgccggagc agatcgccga ccagctcgag
     2581 ctgtggcagc aggccggcgt cgacggcatc aacgtcatca actggcacct gcccggctcc
     2641 tacgaggagt tcaacgaaca cctgctgccg gtgctgcagc agcgcggcct ggccaagcgc
     2701 gagtaccgcc ccggcaccct gcgccagaag ctgttcggca gcgaccgcat caacgagcgc
     2761 catccggccg cccgctatcg cggcgccttc acccccaaga ccgaaacccg caatgtggag
     2821 ttcgcatgaa cgcacgacaa tccggcctgc cccatccctt catcagaacg aagtccggcg
     2881 ctgaaccggc ggtctctccg acggccgtcg cgcccgcgct cgccgcccct gcgccgcagt
     2941 tcgagaccct ggccgcgcgc ttccgcccca tcttcgcccg catcgccgag cgcgccgccg
     3001 aacgcgagca cgaccgcgag ctgcccttcg acgccgtcgg ctggctgcgc gaggcccgct
     3061 tcggcgccct gcgcgtgccg gtggaacacg gcggcttcgg cgcctcggtc gagcagttct
     3121 tcgcgctgct gatcgagctc gccgaggccg actcgaacct gcccaacatc ttccgcgccc
     3181 atttcgggca gatcgagcgc ctgtacgccg agatcgaccc cgccctccat gccccctggc
     3241 tggagcgcgc cgccgccggc gagatcttcg gcaacgccac caccgaaatc ggcgacggcg
     3301 cgctcggcca gccggcgacg cggatcacga agcaggaagg cgtgctgcgc ctgaacggca
     3361 ccaagtacta cagcagcggc tcgctgtacg ccgactggat cgcggtgagc gcgcaactgc
     3421 aggaggaggg cgtggcgggc ggcgccgagc agcccgtcat cgctctggtg cgggcggacg
     3481 cccccggcgt ggagcgcatc gacgactgga agggctttgg ccagcgcctg accggctcgg
     3541 gcaccacccg cctcaccgac gtcgagggcg accccgcctc gatctggcct tgcgcgcgca
     3601 gcgggccgac gtcgatgacc gccctcttcc agctcgtgca ccttgccacg ctgaccggca
     3661 tcgcccgtgc catcgagcgc gatgcctgcg cctacgtgcg tccgcgcaag cgcgtgttca
     3721 gccacggcag tggcgccatc ccgcgcgaag acccgctcat ccagcaggtc gtcggccagc
     3781 tcgccagcac cgccttcatc gccgaggccg ccgtgcgcgc cgtggcagac aggctcggcg
     3841 acatcgaccg cctgcgccgg cgtggcgaag ccgtccccga aagcctgctg gtggacgtcg
     3901 aacttgccgc ggcaaaggcc caggtcggcc tggtcgacgc cgtgctgacc tcgaccacgc
     3961 gactgttcga cgtcggcggc gcctcggccg tgcaggaaaa ccggcggctg gaccgccact
     4021 ggcgcaacgc gcgcacgctg gcctcgcaca acccggtgat cttcaaggcg cgggtagtgg
     4081 gcgaccacgc gatcaacggc accgcgcccg ggttttactg gacggtgggt gcggcgacgg
     4141 ggtgaggcga aggcccgaga tgtcgtcgtg ggtttgttgc gcaatccggg cggcgtcagc
     4201 tgacccgcct tcttgccgca gacagccgat cgtagacctg gcctcccagc catttcgaca
     4261 ccagcgcctg catctccggc ttggccatgt aatctgtgaa cagctcctcg ttcaactcca
     4321 tgcgctcgat gaacagcgac tccagcacct gcctgaacac gagctggaac ttgtcgaggg
     4381 aattgacctc ggccgccctc tgcaacgact cgttcaagct cgccgcctca gcgatctggt
     4441 cgaagaacaa ctggtccgct tcgttgagct cgccgccgaa gcgctggttg atgatgtcga
     4501 tcaggcggga gagcgaaaca tgctcctcgc gcaccatgcc cgagcccacc tcgcgcgggc
     4561 cgtccagcgg tttggcataa ccctcgtcga ggctgatcga gccttcgctg atcttctgca
     4621 ggcggtagta gtcgagctcg acctcttcgt cgaactggta gccgggcccg ctcttgcgct
     4681 tgggcagctt cagcgcgagg tggcgcaggt aggtgaacag cttttccaga tcgctgtcct
     4741 gatacggaat cacctggctc agaaagccgt acagattgcg gaaggcctgc agcttgccgc
     4801 gccacagttc ggcttcctcc tcctccgtgt tctgcaactg cacgaagcgc gcgaccgcct
     4861 ggtcgaggat cgcgttcatc gccttgtggt cgccggggct ctggcggcgc ttgggcgcga
     4921 agaacacccg cgaaaaatcg ctcacttcgg tctgacggta gatgcccgag gcatcgagct
     4981 cggccttgac cgcgtagagc ttgtcgggat cgacctcctc gcccatcacc gcgccttcgt
     5041 agtactggcg gaaggcctcc tggatctcgc ccggctcatt gacgaagtcg agcacgaagg
     5101 tgtcgtcctt gaggggatgg gtgcggttca ggcgcgacag cgtctgcacc gcctgaatgc
     5161 ccgccaggcg cttgtccaca tacatcgtgt gcagcagcgg ctgatcgaag ccggtctggt
     5221 atttctcggc caccagcaac acgcggaaat ccggcttggc gaaggtttcc ggcaaggcct
     5281 tttccttgag gccgccgttc atctccacct cggtataggt cttctcggca atcctgtcgt
     5341 cctcaacgct gcccgagaac gcgaccaggc tcttgatcgg gtagcccttt tcctggatgt
     5401 agcgatcgaa ttcctgcttg tagcgcaccg cctccagccg cgagccggtc accaccatcg
     5461 ccttcgcgtg gccgccgatc ttgtgccgag tgaagtgctg gaagtgttcg accatcacct
     5521 cggtcttttg gccgatgttg tgcgggtgca ggcgcatgaa gcgggccagc gccttggccg
     5581 ccttcttgcg ctcgacgttg gggtcgtcct cggccttctt gatc
//
LOCUS       DAOQNN010000205         5592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8491_length_5592_cov_10.613509, whole genome shotgun sequence.
ACCESSION   DAOQNN010000205 DAOQNN010000000
VERSION     DAOQNN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8491_length_5592_cov_10.613509"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKV97_10960"
     CDS             <1..282
                     /locus_tag="PKV97_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06439.1"
                     /translation="FERKVLARLPMVALVGTAACVAVSLLARLHWWGDESIEAVRALQ
                     MVDIWVLAAVILHWTVVFTVAIGAFIVFVMKGPAYIADRYELPDADAPR"
     gene            complement(337..993)
                     /locus_tag="PKV97_10965"
     CDS             complement(337..993)
                     /locus_tag="PKV97_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNB06440.1"
                     /translation="MSANRIYIYKRYERFWHWSQALLIIVMMITGFEIHGSYTLLGFR
                     KAVDAHTIAAWTLLVIWAFTIFWQFTTGEWRQYVPSMKNVSAMVLYYTVGIFKEMPHP
                     FRKTTVQKHNPLQRLAYLALLAFISPLIWGSGLLYLFYGNWAQLGIDEMFSLEGVALV
                     HTAGAFMITAFFFIHVYLTTTGHTVFAHIKAMITGWEEVDDDHPPAAAEQGGRTPGAA
                     "
     gene            complement(996..2624)
                     /locus_tag="PKV97_10970"
     CDS             complement(996..2624)
                     /locus_tag="PKV97_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HNB06441.1"
                     /translation="MGRRTFDWRRWIGGALILATSMMAGAASAPAGGDLPVAAAGGKA
                     ASTADHSRFKELQGPFESGPEVTKACLACHNEAGHQVMKSVHWTWEAISPTTGKKLGK
                     QHAANNFCGSILSNEPRCTSCHAGYGWTDKNYDFTNQNNVDCLACHDATTTYKKIATD
                     AGHPLYAPRELPKGSGRIVQPPDLARIAQAVGKPGRHNCGACHFNGGGGDAVKHGDLD
                     SSLVKPPKHVDVHMSPDGENMSCADCHTFNAHQPSGSRYAATAKDTKGLDMKHEDHKR
                     ATCESCHDLRPHKNERLDNHARRVACQTCHIPEFARGGIATKVLWDWSTGGKRGPDGK
                     PLFIQDDHGHLIYSAEKGDFKYGENVRPTYKWYNGVVHQIALTDKIDDSKILELNRVE
                     GSASDPNARIWPFKVMVGKQPYDPVNKTLVVNHVYGQDDTAFWGNFDYAKSIKAGMDY
                     AGLPYSGQFGFIETRMNWFITHMVAPKEDALKCRDCHTRSEDGRLAGITDLYLPGRDR
                     NGWVDLLGGLAIIGAIGAGSIHGIARILTRRKGH"
     gene            2829..4673
                     /locus_tag="PKV97_10975"
     CDS             2829..4673
                     /locus_tag="PKV97_10975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB06442.1"
                     /translation="MSTPSNPSEIARETLRQLALRRIAPTPDNYRKLYHEIAGTRPDD
                     DALPEAFVRKLARRLPRDSAERQRHGHQLDQALADGRDKAAEAALERYLDSLKLGEPQ
                     AWNELIGNLLRQWEARQTGWTPARKREALDRVLGAADPAALYTRLQGLLKSWTQAMTD
                     ADALAVEVPDAADEPDASASPRADASTARPATPARTAAAAPPPASTPGVDSATIDALR
                     ELVTISLGEALPPLLEEQPPLRETAATLCAEAASRSEAAGLREVGTRLRAFAHELELA
                     AADEAEIRAGMLELLRMLLRNIDELVLDDRWLAGQIEMLREIVDSRPDPRKIDDAGRR
                     LKELIHKQGQLKHTLAQSQRHLRDMLAGFVDQLARFSESTGAYHDRIGQCAQRIAQAN
                     DITEIGPLLDEVMTETRSIQDEARRSRDELIVARGEATAAQERVVTLQNELEEASRLM
                     RHDQLTGALNRRGLDEMFQTESARAQRRGTRLAVALLDIDHFKKLNDSLGHKGGDDAL
                     VHLAKVVRQHLRPQDVLARYGGEEFVILLPETGDTDAQQALVRLQRELTREFFMAEMQ
                     KIVITFSAGVTRLAEGESLESALKRADGAMYQAKQAGRNRVMVAPLHD"
     gene            complement(4752..>5592)
                     /locus_tag="PKV97_10980"
     CDS             complement(4752..>5592)
                     /locus_tag="PKV97_10980"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB06443.1"
                     /translation="TGEERIMRQACASILATAALVTALGSGTAEAAVLDVTVRGSDAI
                     FLAGRTDITIPLASAPWSSPTGMQRHGGATPEEIQETFPPYLGVAAGDVIRLADPSIG
                     GISYFNGVGAPFYGPEGNNADCAGSSCSDLSSFGGISGYFGPEGALVGVFLDNSIPSA
                     GAPARLDFSTTGLGRNFTTLSPGLGQIFYIGNGVTTSSIFQEFIAPSGATRLFLGIPD
                     GFGFDGVPGAYDDNDGAYRVRIGVNEVPTVPEPGVLALLGLGLVGLAAARLRTPQGSK
                     VPG"
BASE COUNT          911 a         1963 c         1825 g          893 t
ORIGIN      
        1 ttcgaacgca aggtgctggc gcgcctgccc atggtcgcgc tcgtcggcac cgcggcctgc
       61 gtggccgtgt ccctgctcgc ccggctgcac tggtggggcg acgagagcat cgaggcggtg
      121 cgcgcgctgc agatggtcga catctgggtg ctggcagcgg tgatcctgca ttggacggtg
      181 gtgttcaccg tggcgatcgg tgccttcatc gtgttcgtga tgaagggccc ggcctacatc
      241 gccgaccgct acgaactccc ggacgcagac gccccgcgct gaatccgccg cggggcgtct
      301 gcgtcccttg gtggtgccgc gatacggcgc aagcactcaa gccgcgccgg gtgtgcgccc
      361 gccctgctcg gccgcggcgg gcgggtggtc gtcatcgacc tcttcccagc cggtgatcat
      421 cgccttgatg tgcgcgaaga ccgtgtgccc cgtggtcgtc aggtacacgt ggatgaagaa
      481 gaacgcggtg atcatgaacg cacctgcggt atgcacgagc gccacgccct cgagcgagaa
      541 catctcgtcg atgccgagct gcgcccagtt gccatagaac aggtaaagca ggcccgagcc
      601 ccagatcagc ggcgagatga acgcaagcag cgcgagatag gccaggcgct gcagcgggtt
      661 gtgcttctgc accgtcgtct tgcggaaggg atgcggcatc tccttgaaga tccccaccgt
      721 gtagtagagg accatcgccg acacgttctt catcgacggg acgtactgcc gccactcgcc
      781 cgtggtgaat tgccagaaga tcgtgaaggc ccagatcacc agcagcgtcc atgccgcgat
      841 cgtgtgcgca tccacggcct tcctgaagcc caaaagagta tagctgccgt ggatctcgaa
      901 gccggtgatc atcatgacga tgatcagcag cgcctgcgac cagtgccaga agcgttcata
      961 gcgcttgtag atgtagatgc ggttcgcgct cattttcagt gccccttgcg acgggtgagg
     1021 atgcgggcga tgccgtggat cgagccggcg ccgatcgcgc cgatgatcgc cagcccaccg
     1081 aggagatcga cccagccgtt gcggtcgcga ccgggcaggt agaggtcggt gatcccggcg
     1141 aggcggccgt cttccgagcg ggtgtggcag tccctgcact tgagcgcgtc ttccttgggc
     1201 gccaccatgt gggtgatgaa ccagttcatg cgcgtctcga tgaagccgaa ctggccgctg
     1261 tagggcaggc cggcgtagtc catgccggcc ttgatcgact tggcgtagtc gaagtttccc
     1321 cagaacgcgg tgtcgtcctg gccgtatacg tggttcacca cgagagtctt gttcaccggg
     1381 tcgtagggct gcttgccgac catcaccttg aagggccaga tgcgcgcgtt cgggtcgctc
     1441 gccgagcctt cgacgcggtt cagctcgagg atcttgctgt cgtcgatctt gtcggtgagc
     1501 gcgatctgat ggaccacgcc gttgtaccac ttgtaggtcg ggcgcacgtt ctcgccgtac
     1561 ttgaagtcgc ccttttccgc cgagtagatc aggtggccgt ggtcgtcctg gatgaacagc
     1621 ggcttgccgt ccgggccgcg cttgccgccg gtcgaccagt cccacagcac cttggtggcg
     1681 atgccgccgc gcgcgaactc ggggatgtgg caagtctggc aagccactcg cctggcgtgg
     1741 ttgtcgaggc gctcgttctt gtgcgggcgc aggtcgtggc aggactcgca ggtcgcgcgc
     1801 ttgtggtcct catgcttcat gtccaggccc ttggtgtcct tggcggtagc cgcgtagcgg
     1861 ctgccggagg gctggtgggc gttgaaggtg tggcagtccg cacagctcat gttctcgcca
     1921 tcgggcgaca tgtgcacatc gacgtgtttc ggcggcttga ccagcgagga gtcgaggtcg
     1981 ccatgcttga ccgcgtcgcc gccaccgccg ttgaagtggc acgcgccgca gttgtgccgc
     2041 cccggcttgc ccaccgcctg cgcgatcctg gccaggtcgg gcggctgcac gatcctgccg
     2101 ctgcccttgg gcagctcgcg cggcgcgtag agcggatggc cggcatcggt ggcgatcttc
     2161 ttgtaggtcg tcgtcgcgtc gtggcaggcc aggcaatcca cgttgttctg gttggtgaaa
     2221 tcgtagttct tgtccgtcca cccgtagccg gcgtggcagc tcgtgcagcg cggctcgttg
     2281 gaaaggatgg agccgcagaa gttgttggcc gcgtgctgtt tgcccagctt cttgccggtg
     2341 gtcgggctga tcgcctccca ggtccagtgc acgctcttca tcacctggtg cccggcctcg
     2401 ttgtgacagg cgagacaggc cttggtgacc tccggcccgc tctcgaacgg gccctgcagt
     2461 tccttgaacc tgctgtggtc cgccgtgctc gccgccttgc cgccggccgc agccaccgga
     2521 agatcgcccc ctgcgggcgc gcttgccgca cccgccatca tgctcgtcgc gaggatcaag
     2581 gccccgccga tccagcgccg ccagtcgaat gtccgcctgc ccattttccg ttcctccgcc
     2641 gagacgacct gcttttccag tctaggccag ccagaaatat aagcatttga ttaatatcta
     2701 cggatcggca gatcaccggg gctcgactcc ctccgtgccc gccctcggaa cttcctcacc
     2761 gaaagcacgg gaagtcctgt cagaatgccg gaaacatgcg ccagacccag gaatttccac
     2821 cgctcaacat gtccacgccc agcaatccct ccgagatcgc ccgcgaaacc ctgcgccagc
     2881 tcgcgctgcg ccggattgcc cccacgccgg acaactaccg caagctctat cacgagatcg
     2941 ccggcacccg gcccgacgac gacgccctgc ccgaagcctt cgtgcgcaaa ctcgcccgcc
     3001 gcctgccgcg cgacagcgcc gagcgccagc gccacggcca ccagctcgac caagcgctcg
     3061 ccgacggtcg cgacaaggcc gccgaggccg cactcgagcg ctacctcgac agcctcaagc
     3121 tgggcgaacc gcaggcctgg aacgagctca tcggcaacct gctgcgccag tgggaggcgc
     3181 gtcagaccgg ctggaccccg gcgcgcaagc gcgaggccct cgaccgcgtg ctgggcgcgg
     3241 ccgacccagc ggcgctgtat acccgcctgc agggcctgct caagagctgg acgcaggcca
     3301 tgaccgatgc cgatgcgctc gccgtcgagg tgcccgacgc ggcagacgaa cccgacgcca
     3361 gcgcttcgcc ccgtgccgac gcaagcaccg caaggcccgc cacgcccgcg cgcaccgccg
     3421 ctgccgcgcc cccccctgcc tccactccag gcgtcgactc cgccaccatc gacgccctgc
     3481 gcgagctcgt caccatcagc ctgggcgagg ccctgccgcc gctgctggag gaacaaccgc
     3541 cgctgcgcga gaccgccgcc acgctgtgcg ccgaagctgc cagtcgcagc gaggccgctg
     3601 gcttgcgcga ggtcggcacg cgcctgcgcg ccttcgcaca cgagctcgag ctggccgccg
     3661 ccgacgaggc cgaaatccgc gccggcatgc tcgagctgct gcgcatgctg cttcgcaaca
     3721 tcgacgaact ggtgctcgac gatcgctggc tcgccggcca gatcgagatg ctgcgcgaga
     3781 tcgtcgacag ccgtcccgac ccgcgcaaga tcgacgacgc cggccgccgc ctcaaggaac
     3841 tcatccacaa gcagggccag ctcaagcaca ccctggccca gtcccagcgc cacctgcgcg
     3901 acatgctcgc cggcttcgtc gaccagctcg cgcgcttctc cgaatcgacc ggcgcctacc
     3961 acgaccgcat cggccagtgc gcccagcgta tcgcccaggc caacgacatc accgagatcg
     4021 gccccctgct cgacgaggtg atgaccgaga cgcgcagcat ccaggacgag gcacgccgct
     4081 cgcgcgacga gctcatcgtc gcccgcggcg aggccacggc ggcgcaggag cgcgtcgtca
     4141 cgctgcagaa cgagctcgag gaagccagcc gcctgatgcg ccacgaccag ctcaccggcg
     4201 cgctcaaccg ccgtggcctc gacgagatgt tccagaccga atccgcgcgc gcccagcgtc
     4261 gcggcacgcg gctcgccgtc gcgctgctcg acatcgacca cttcaagaag ctcaacgaca
     4321 gcctcggcca caaaggtggc gacgacgccc tggtacacct cgccaaggtc gtgcgccagc
     4381 acctgcgccc ccaggacgtg ctcgcccgct atggtggcga ggagttcgtc atcctgctgc
     4441 ccgagaccgg cgacaccgac gcccaacagg cgctcgtgcg cctgcagcgc gagctcaccc
     4501 gcgaattctt catggccgaa atgcagaaga tcgtgatcac cttcagcgcc ggcgtcaccc
     4561 gcctggccga gggcgaaagc ctggagagcg cgctgaagcg cgccgacggc gcgatgtatc
     4621 aagccaagca ggccggccgc aaccgcgtga tggtcgcgcc gctacacgac tgaaagcgct
     4681 ccggagcaca cgaaacccgc cggcttcgcc aagggcgccg agcccgtggc gaagccggcg
     4741 cggcggcgcg ctcagcccgg caccttgctc ccttgaggag tgcgcaggcg cgcggcggca
     4801 aggccgacga ggcccagccc gaggagcgcg agcacgcccg gctccggcac ggtgggcacc
     4861 tcgttgacgc cgatgcggac gcggtaggcg ccatcgttgt cgtcgtaggc accgggcacg
     4921 ccatcgaagc cgaagccgtc cgggatcccc aggaacagcc tcgtggcgcc gctcggcgcg
     4981 atgaactcct ggaaaatcga ggacgtggtc acgccgttgc cgatatagaa gatctgcccg
     5041 agcccggggc tcagtgtcgt gaagttgcgg cccaggccgg tcgtgctgaa gtcgaggcgc
     5101 gcgggtgcgc cagcgctggg gatgctgttg tccaggaaga cgccgacgag tgccccctcg
     5161 ggaccgaagt agccactgat gccgccgaag gacgagagat cgctgcagct cgaccccgcg
     5221 caatcggcgt tgttgccctc ggggccatag aacggcgcac cgaccccgtt gaagtagctg
     5281 atgccgccga tcgagggatc cgcgaggcgg atgacgtcgc ccgcagccac gccgaggtat
     5341 ggcgggaagg tctcctggat ctcctcgggc gtagcccccc cgtggcgttg catgccggtg
     5401 ggagagctcc aaggcgcaga cgccagcgga atcgtgatgt ccgttctccc cgccaggaag
     5461 atcgcgtcgc tgccgcgaac cgtgacatcg agcacggccg cctctgccgt gccggaaccg
     5521 agcgccgtga ccagcgccgc agttgcaagg atcgatgcgc aagcctgcct catgattcgc
     5581 tcctccccgg ta
//
LOCUS       DAOQNN010000206         5592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8492_length_5592_cov_7.065198, whole genome shotgun sequence.
ACCESSION   DAOQNN010000206 DAOQNN010000000
VERSION     DAOQNN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8492_length_5592_cov_7.065198"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5592)
                     /locus_tag="PKV97_10985"
     CDS             complement(<1..>5592)
                     /locus_tag="PKV97_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukotoxin LktA family filamentous adhesin"
                     /protein_id="HNB06444.1"
                     /translation="KTTHAWIDDGAAASALGNAAAAQVADGGFDIDYVATAAGEGEVA
                     APGITPSDGENDLAGGSAALDATRTSSASTSAHRGLAVTAVNRDDIKGFAVTGAAAGT
                     AAVTLSGEVSVHQTDTRASIGSIALAEGASHTGVQVNADNTGANAAQSVRVAAGNDSF
                     HLGIAGALSASGTVGVGVGADVAAMRHATHAAIVEQSDVKARRDVEVAASSGQEVVSI
                     SASLGASGTVGVAGSVSVLSFDNATSASIGDEAEVAAGGNVSVKADDHTATTLVAGTI
                     ALGIGGGGVGAGVGVTLIEKDTRAWIGAGAVVDALGKDTADLVVRSGESVDETATARG
                     VQVQAESSEDLFTVSAAAAGGLYLGFAGAVSVATVDSDTAAWIGADARVNRGGGDADA
                     AQDVNVGARNELALFNVSGALGVGAAGIAGGVDVGVIRNDTTAWIGDRAEVGARRDIG
                     VSALANTDIESYVVSAAGGIGALAGGVGVYSVGGGLGDEAQGRLESGDDNAGSYVDGQ
                     ATDGSVATLLGSYTDSRIGEAADRVEDERGTLAASGRFTAAETRTLPAGTAAFVGENA
                     DLEAGRDIDLDARTDVEFDALDGALAIGAIGLGAGVGVADFDNDTLASVGRGSTLRAG
                     AAGSVSLTAALDEDVDVTAFAGTGGIVAVDAAVALIGSTGAVQAGLDDEVAIERANAV
                     DIAADDTRRLEAETTGVSVGVVAAGASVTGSTIGGGATASIGDEVAIGQVAGDTVGEL
                     HVLADASHTAVATALAAKGGLGLAASGTVADAVVDTNVSASIGDDARIKVARTVEVDA
                     NSAGAARADATGINVSLGGAVGASIARATLSPTVSATIGQRSLIDAADFTLAAHQALP
                     AGGGRSAFASASGAAGGLLVGANASRSTAFNDAAVNARVGDDSTLKISGTATVQTHNT
                     HSQLAYVSGVSAGFVAVGANTASAGSNTDSHAALGDHVRVEGGTLNVLADGQATNHAY
                     GVAGAGGLVSVPFSASYTTTVSRTSATTGSKAARQPGDPATIAVDSLTLAATQGSSFD
                     AWMSNTNASLVGVSGAQTENFARATTTATIGAEGEIEAGTVQLDASTTLSKGAPAGLL
                     AGTGLARPDWNVESSSGGLADFPAAGSRTILLADTLAGIGAGATVLSTSGNAATPGIR
                     VDARNEIGASDRVKMASGGAVSAASGESVVSTVCPPEHAACNAGERPAATVRVGDGAH
                     VETTGESDIAMGSRTSANLHSQSAVDVYGLVGVAPEGSSTAVYDGDNRIEIGDADLRS
                     ERNIRLAAGAASDGTANALGASARTDVFNNTAIPVNRDPVADAFVTSRSAVSVAAGAD
                     VAAVRNVALHAVEGSAGASGVGIGKDIYRETLAAIASWFSNLFGGGDVSFETRTGRSG
                     VSQDSTVDVRGTVRVGIERRQRLHIDYDGSVIEQTDGIGIASTRMQDIYADILARIET
                     LRALIRDYAVGPGDTTSDAAIATAAYRAEISFLEHKLHEMGFGGNPDEPGFNDSNPRQ
                     MAEDRVGALDGQLGGLRTTRTQLQAEKTTIEGQVATLNQPLDEDNGTRTAQNTTLSNQ
                     ITGLNAQIAALDPDDDDYATDLAALRGQVATRQAAIDANNRAITANNQQIALNNAPLD
                     AKNAQIAAVNTEIGSLETTVGKIRQGIAAGDYGDASGAGLQAKFLRIGDSVARLGDID
                     VRADRLTGTGELDAPGDAGIHIINEGPSFLTLGKLEIASDAGGRLVFNGVEVRDNAGI
                     AAINRGAGTAAFARIVTAEAGSDAPAILVENRFNPLDDADATRAIGAGVAPVAPDIVL
                     TDAITNLRGLVGIHSEAGGIRLEQTASIAANSVEVQALNGDFVQSYTDSFTHVATSPL
                     KEVVGSGGTRSIVQDTSGSGSI"
BASE COUNT          616 a         2032 c         2058 g          886 t
ORIGIN      
        1 gatgctgccg ctgccgctgg tgtcctgcac gatggagcgg gtcccccccg agccgaccac
       61 ctccttgagc ggcgaggtgg ccacgtgggt gaagctgtcg gtgtagctct gcacgaagtc
      121 gccgttgagg gcctgcactt cgacgctgtt ggccgcgatc gacgcggtct gctcgagccg
      181 gatgccgccg gcctcgctgt ggatgccgac caggccgcgc aggttggtga tcgcgtcggt
      241 gagcacgatg tcgggggcca ccggcgccac gccggcgccg atcgcgcgcg tggcgtcggc
      301 gtcgtcgagc ggattgaagc ggttctcgac caggatggcg ggggcgtcgc tgcccgcctc
      361 ggcggtgacg atccgcgcga acgcggccgt gcccgcgccg cggttgatcg cggcgatgcc
      421 ggcgttgtcg cgcacctcca cgccgttgaa gaccagccgg ccacccgcgt ccgaggcgat
      481 ctcgagcttg cccagagtga ggaaggacgg cccctcgttg atgatgtgga tgccggcgtc
      541 gcccggcgcg tcgagctcgc cggtgccggt gaggcggtcg gcgcgcacgt cgatgtcgcc
      601 cagccgggcc acgctgtcgc cgatgcgcag gaacttcgcc tgcaggccgg cgccgctggc
      661 gtcgccgtag tcgccggcgg cgatgccctg gcggatcttc ccgaccgtgg tctcgaggct
      721 gccgatctcg gtgttcaccg cggcgatctg ggcattcttg gcgtcgagcg gcgcgttgtt
      781 gagcgcgatc tgctggttgt tggcggtgat cgcgcggttg ttggcgtcga tggccgcctg
      841 ccgggtggcg acctggcccc gcagggcggc gagatcggtc gcgtagtcgt cgtcgtccgg
      901 atccagcgcc gcgatctgcg cgttcaatcc cgtgatctgg ttgctcagcg tcgtgttttg
      961 cgccgtccgc gtgccgttgt cctcgtcgag tggctggttg agcgtggcga cctggccctc
     1021 gatcgtggtc ttctcggcct gcaattgcgt gcgcgtggtg cgcaggccgc ccagctgtcc
     1081 atcgagcgcg cccacgcggt cctcggccat ctggcgcgga ttgctgtcgt tgaagccggg
     1141 ctcgtccggg ttgccgccga agcccatctc gtggagtttg tgctccagga aggagatctc
     1201 cgcccggtag gccgcggtgg cgatcgccgc atcgctggtg gtgtcgcccg ggcctaccgc
     1261 atagtcgcgg atcagcgccc gcagggtctc gatgcgcgcc aggatgtccg cgtagatgtc
     1321 ctgcatccgt gtcgatgcga tgccgatgcc gtcggtctgc tcgatcaccg aaccgtcgta
     1381 gtcgatgtgc aggcgctgcc tgcgctcgat gcccacgcgc acggtgccgc ggacgtcgac
     1441 cgtgctgtcc tggcttacgc cggagcgtcc ggtgcgggtc tcgaaggaga cgtcgccgcc
     1501 gccgaagagg ttgctgaacc agctcgcgat cgccgccagg gtctcgcggt agatgtcctt
     1561 gccgatgccc acgcccgagg cgcccgccga gccttccacg gcgtgcaggg ccacgttgcg
     1621 caccgcggcc acgtcggcgc cggcagcgac gctcaccgcg ctgcggctgg tgacgaaggc
     1681 gtcggccacc gggtcgcggt taaccgggat cgcggtgttg ttgaagacgt cggtgcgcgc
     1741 gctcgcgccg agcgcgttcg cggtgccgtc gctcgcggcg ccggccgcca ggcggatgtt
     1801 gcgttccgaa cgcagatcgg cgtcgccgat ctcgatgcgg ttatcgccgt cgtacaccgc
     1861 ggtgctgctg ccctcggggg cgacgccgac caggccgtag acgtccaccg cgctctgcga
     1921 atggagattt gcgctggtcc gcgagcccat cgcgatgtcg ctctcgcccg tggtctcgac
     1981 gtgggcgccg tcgccgacgc gcacggtcgc ggccgggcgt tcgcccgcgt tgcaggcggc
     2041 gtgctccggc gggcacacgg tcgacacgac cgactcgccg ctcgctgccg acaccgcgcc
     2101 gcccgaggcc atcttgaccc ggtcgctggc accgatctcg ttgcgcgcat cgacgcggat
     2161 ccccggcgtg gcggcattgc cgctcgtgga gagaaccgtc gcgcccgcgc cgatcccggc
     2221 gagcgtgtcc gcgagcagga tcgtgcggct gccggcggcg gggaagtcgg cgagcccgcc
     2281 cgagctcgac tccacgttcc agtccgggcg tgccaggccc gtgccggcga gcaggccggc
     2341 aggcgccccc ttgctcagcg tcgtgctggc gtcgagctgc accgttccgg cttcgatctc
     2401 gccctcggcg ccgatcgtcg cggtggtggt ggcgcgcgcg aagttctcgg tctgcgcgcc
     2461 gctcacgccg accagcgacg cgttggtgtt gctcatccag gcgtcgaagc tggagccctg
     2521 cgtcgccgcg agcgtcaggc tgtcgaccgc gatcgtcgcc gggtcgccgg gctgacgcgc
     2581 ggccttgctg ccggtcgtgg cgctcgtcct gctcacggtc gtggtgtagc tcgccgagaa
     2641 cggcaccgag accagcccgc cggcaccggc cacaccgtag gcatggttgg tggcctggcc
     2701 gtcggcgagc acgttcagcg tgccgccctc gacccggaca tggtcgccga gcgcggcatg
     2761 gctgtcggtg ttcgagcccg cgctggcggt gttggcgccg accgccacga agcccgccga
     2821 cacgccggac acgtaggcga gctgggagtg cgtgttgtgc gtctgcacgg tggcggtgcc
     2881 gctgatcttc agcgtgctgt cgtcgcccac cctggcgttc accgcggcgt cgttgaaggc
     2941 ggtggagcgc gaggcgttcg cccccaccag caggccgccg gcggcgccgc tggcgctggc
     3001 gaaggcgctg cgtccgccgc cggcgggcag ggcctggtgc gcggccagtg tgaagtcggc
     3061 ggcgtcgatg aggctgcgtt gaccgatcgt ggcggacacc gtgggtgaca gggtggcgcg
     3121 cgcgatcgag gcgccgaccg ccccgcccag gctcacgttg atgccggtgg cgtcggcgcg
     3181 tgccgcgccg gcgctgttcg cgtcgacctc gaccgtgcgg gcgaccttga tccgggcgtc
     3241 gtcgccgatg cttgcggaga cgttcgtgtc gaccaccgcg tcggccaccg tgccgctggc
     3301 ggccagcccg agcccgccct tggcggcgag cgcggtggcg acggcggtgt ggctggcgtc
     3361 ggcgagcaca tgcagctcgc cgaccgtgtc gcctgcgacc tggccgatgg ccacctcgtc
     3421 gccgatgctg gcggtggcgc cgccaccgat cgtgctgccg gtcaccgagg cccccgcggc
     3481 gaccacgccg acgctgacgc cggtggtctc ggcctccagt ctgcgggtgt cgtcggccgc
     3541 gatgtcgacc gcattggcac gctcgatcgc gacctcgtcg tcgaggccgg cctgcaccgc
     3601 gccggtggag ccgatcagtg cgaccgccgc gtcgaccgcg acgatgccgc cggtgccggc
     3661 gaaggcggtc acgtccacat cctcgtcgag cgccgcggtc aggctcacgc tgccggcggc
     3721 gccggcacgc agggtgctgc cccggccgac ggaggcgagg gtgtcgttgt cgaaatccgc
     3781 gacccccacg ccggcgccga ggccgatcgc gccgatggcg agggcgccgt cgagcgcatc
     3841 gaactcgacg tccgtgcgtg cgtcgaggtc gatgtccctg cccgcctcga ggtccgcatt
     3901 ctcgcccacg aaggcggcgg taccggcggg cagggtgcgg gtttccgccg cggtgaagcg
     3961 gccgctcgcg gcgagcgtgc cacgctcgtc ctccacccga tcggcggcct cgccgatgcg
     4021 gctgtccgtg tagctcccga gcagggtggc gacgctgccg tcggtggcct ggccatcgac
     4081 atagctgccg gcattgtcgt cgccggactc gagccggccc tgcgcctcgt cgcccagccc
     4141 accgcccacg gaatacacgc cgacgccgcc ggcgagcgcg ccgatccccc cggcggcgct
     4201 caccacgtag ctctcgatgt cggtgttcgc gagcgcgctc acgccgatgt cgcgcctggc
     4261 gcccacttcg gcgcggtcgc cgatccaggc ggtggtgtcg ttgcggatca ctcccacatc
     4321 cacgccgccg gcgatgccgg ccgcgccgac gccgagcgcg ccggagacgt tgaagagcgc
     4381 caactcgttg cgcgcaccga cgttgacgtc ctgcgccgcg tcggcgtcgc caccgccccg
     4441 gttcacccgg gcgtccgcgc cgatccaggc cgcggtgtcc gaatccaccg tcgccaccga
     4501 caccgcgccg gcgaagccga ggtagagccc gcccgcggcg gcggccgaga cggtgaacag
     4561 atcctcgctc gactccgcct gcacctgcac gccgcgcgcc gtggcggtct cgtccacgct
     4621 ctcgcccgag cgcacgacca gatcggcggt gtccttgccg agggcatcca cgaccgcgcc
     4681 cgcgccgatc caggcgcgcg tgtccttctc gatcagcgtc acgcccacgc cggcgccgac
     4741 tccgccaccg ccgatgccga gcgcgatcgt gccggcgacc agggtcgtgg cggtgtggtc
     4801 gtccgccttc acgctcacgt tgccgccggc ggcgacttcg gcttcgtcgc cgatcgaggc
     4861 cgaggtcgcg ttgtcgaagc tcagcaccga cacgctgccg gccaccccga ccgtgcccga
     4921 ggcgcccagg ctggcgctga tcgagaccac ctcctgaccg gaactggcgg cgacctcgac
     4981 gtcgcgccgc gccttgacgt cgctctgctc gacgatggcc gcatgggtgg cgtgccgcat
     5041 cgccgccaca tcggcgccga cgcctacgcc caccgtgccc gaggccgaca gcgcgccggc
     5101 gatgccgagg tggaaactgt cgttgccggc ggcgacgcgc accgattgcg cggcgttggc
     5161 gccggtgttg tcggcgttga cctgcacgcc ggtgtggctg gcgccctcgg cgagcgcgat
     5221 gctgccgatc gaggcgcggg tgtcggtctg gtgcaccgac acttcgcccg acagggtcac
     5281 cgccgcggtg ccggctgcgg cgccggtgac cgcaaagccc ttgatgtcgt cgcggttcac
     5341 cgcggtcacc gcgagcccgc ggtgcgcgct cgtgctcgcg ctgctggtgc gtgtggcgtc
     5401 gagcgccgcg ctgccgccgg cgaggtcgtt ctcgccgtcg gagggggtga tgccgggggc
     5461 ggcgacctcg ccttcacccg cagccgtggc cacgtagtcg atgtcgaagc cgccgtccgc
     5521 cacctgtgcc gcggcggcgt tgccgagcgc gctcgccgcg gcgccgtcgt cgatccaggc
     5581 gtgggtggtc tt
//
LOCUS       DAOQNN010000207         5585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8508_length_5585_cov_6.977939, whole genome shotgun sequence.
ACCESSION   DAOQNN010000207 DAOQNN010000000
VERSION     DAOQNN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8508_length_5585_cov_6.977939"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /locus_tag="PKV97_10990"
     CDS             complement(<1..1562)
                     /locus_tag="PKV97_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB06445.1"
                     /translation="MKVGMKLGLGFGLVLVLLGTLLAVGILRLTDIKHDVDVVVDELW
                     PKTQLLQDGLVGVNEIALGGRELVIAENGEQIGRAKDRMLGGRERIGKAWDALAPMLV
                     QPRGKELFAEVLDSRQRFIPAQNRLIELVEQGRADEARAFLRGDYDAIAVQYRQRVNA
                     LTAYQGELLSGTGSATAATVAAATLTMSVLGGIALLVGVAVAILITRGLLRQLGGEPD
                     YAAEIARKVAAGDLTVDVQLRAGDSASVLAAMQRMVGSLSHTLGEVRGAADALSAASE
                     QVSATSQSLAQGSSEQAASLEETSASIEQMSASIGQNTENARVTDGMASKSAADADEG
                     GDAVRATVEAMRSIADKIGIVDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVRK
                     LAERSQVAAQEIGELAGSSVKTAERAGALLEQIVPSIRKTAELVQEITASSEEQSAGA
                     GQINIAMSQISQTTQQNASASEELSATAEEMNSQAEQLQRLVAQFRLDPQAQLAGGDG
                     GAARTTRGQPAPA"
     gene            complement(1610..3811)
                     /locus_tag="PKV97_10995"
     CDS             complement(1610..3811)
                     /locus_tag="PKV97_10995"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNB06446.1"
                     /translation="MDLSQALATFLDESRELLAQMEEILLRAETAALPTDELHALFRC
                     AHTIKGSAGLFGLDAVVHFTHAVESVLDRMRNGELELSDALINLLLESQDHIASLVAA
                     VAAELPPPEFEGAPLRARLEAFCATTPATPAALPATVAEGDPIVCSATEVAPALEVGG
                     SVALGAEHWHLSLRFAPEVLRNGMDPLAFIHYLRTLGELVHVETIADTLPELAAGDPE
                     NCYLGFEIALRSTAPRAEIEAVFEFVQDSARIVLVPPAARVEEYLALIEALDEDPRRL
                     GEILVTCGSVTARELEAALAAQHVQAEAPRVGELLVEHAGVPAQVVQAALGKQKAAEE
                     RRASETRSVKVPADRLDALIDQVGELVIAGAATQLQAQQAGSRALQETAANLLRLVED
                     VRDAALRLRMTPIGEVFNRFPRVVRDLARELGKDIELVIRGAEAELDKSMVEKIGDPL
                     MHLVRNALDHGIESPERRAAAGKPARGRVELRAFHESGSIVIEVADDGGGLDARRILA
                     KAVERGLVAADAVLSEAEIHRLVLAPGFSTAAELTSLSGRGVGMDVVKSSVDALRGTL
                     DIHSAPGQGTTMRLCLPLTLAIIDGFQVGVAGATFILPLDAVVECIELPADAGAAGYL
                     DLRGQVLPFLRLRALFALEGAPPPRQNVVVVRFGGRQAGIAVDHLLGECQAVIKPLGP
                     LFERVAGIAGSTILGSGEVALILDVPQLVQRAIAHEGRDTGGAPRARFLAA"
     gene            complement(3811..4146)
                     /locus_tag="PKV97_11000"
     CDS             complement(3811..4146)
                     /locus_tag="PKV97_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB06447.1"
                     /translation="MDTPALDTPAAGVARLAPEGELGIYTVAALREAWLQALATHEAL
                     EIDLGRIDEIDTAGLQLMLMAKRSAGRSVRFVNHSAAVLRLLDLARLGGALGDPLLED
                     AAPATQEAR"
     gene            complement(4158..4523)
                     /locus_tag="PKV97_11005"
     CDS             complement(4158..4523)
                     /locus_tag="PKV97_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB06448.1"
                     /translation="MSKTVLIVDDSASLRQVVSLSLKGAGYQVIEACDGRDALGKLGG
                     QKVHLIISDVNMPNMDGIAFVREVKKNPSYKFTPVIMLTTEAAEEKKRLGQEAGARAW
                     VVKPFQPQQMLAAVSKLVM"
     gene            complement(4541..>5585)
                     /locus_tag="PKV97_11010"
     CDS             complement(4541..>5585)
                     /locus_tag="PKV97_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB06449.1"
                     /translation="LPWWILRRERARQADVAARVATAERARLAEREAYLASLQQLAQA
                     ALARWHDHVGISRSQTETAVGALAGEFAHILERLQDALLRSRQSTADRGEDSVVGVIG
                     HARGELDRVLAGLNAALAEKQSLSQAVARLAQVTEELMRMASEVGEIAKQTNLLALNA
                     AIEAARAGEVGRGFAVVADEVRKLSDLSGATGERIRAKVESAHAAMAEALAAAEHMAR
                     GDAVLVRDSEAAIGSVVGRFDQVGNAMAQAARALEEDSTAVQSRIEGVLVQLQFQDRV
                     SQILEAVRTDMARLAAQVAEDEARLAGGRVPAPIDVAAWIAALERTYTTLEQHARRAD
                     ANPAPAGGDITFF"
BASE COUNT          703 a         1982 c         2084 g          816 t
ORIGIN      
        1 gcaggcgccg gctggccgcg ggtggtgcgc gcggcgccgc catcgccgcc ggcgagctgc
       61 gcctgcgggt cgaggcggaa ctgcgccacc aggcgctgca gctgctcggc ctggctgttc
      121 atctcctcgg cggtcgccga cagctcctcc gaggccgagg cgttctgctg cgtggtctgg
      181 ctgatctggc tcatggcgat gttgatctgc cccgcgccgg ccgactgctc ctcggacgag
      241 gcggtgatct cctgcaccag ctcggcggtc ttgcggatcg agggcacgat ctgctcgagc
      301 agcgcacccg cgcgctcggc ggtcttcacg ctggagccgg ccagttcgcc gatctcctgc
      361 gccgcgacct ggctgcgctc ggcgagcttg cgcacctcgg ccgccaccac cgcgaaaccc
      421 ttgccgtgct cgccggcacg cgccgcctcg atcgcggcgt tgagcgcgag caggttggtc
      481 tggtaggcga tgtcgtcgac gatgccgatc ttgtcggcga tcgaacgcat cgcctccacc
      541 gtcgcgcgca ccgcatcgcc gccctcgtcg gcgtcggcgg cggacttgct cgccatgccg
      601 tcggtgacgc gggcgttctc ggtgttctgg ccgatcgagg ccgacatctg ctcgatcgag
      661 gccgaggtct cctccaggct ggcggcctgc tcggacgagc cctgcgccag cgactgcgag
      721 gtcgcgctca cctgctcgga ggccgccgac agggcgtcgg ccgcgccgcg cacctcgccc
      781 agggtgtgcg acaggctgcc caccatgcgt tgcatggccg ccagcacgct ggcgctgtcg
      841 ccggcgcgca actgcacgtc caccgtgagg tcgccggcgg cgaccttgcg cgcgatctcc
      901 gcggcgtagt cgggctcgcc gccgagctgg cgcagcaggc cgcgggtgat caggatcgcc
      961 accgccacgc ccaccagcag ggcgatgccg cccagcaccg acatggtcag cgtggccgcg
     1021 gcgacggtcg cggcggtcgc gctgccggta ccggagagca gttcgccctg gtaggcggtg
     1081 agcgcgttca cgcgctggcg gtactgcacc gcgatggcgt cgtagtcgcc gcgcaggaag
     1141 gcgcgcgcct cgtcggcacg gccctgctcg acgagctcga tgaggcggtt ctgcgccggg
     1201 atgaagcgct ggcggctgtc gagcacctcg gcgaagagct ccttgccgcg cggctgcacc
     1261 agcatcggcg cgagcgcgtc ccaggccttg ccgatgcgct cgcgcccgcc gagcatgcgg
     1321 tccttggcac ggccgatctg ctcgccgttc tcggcgatga ccagctcgcg cccgccgagg
     1381 gcgatctcgt tgaccccgac cagtccgtcc tgcagcagct gcgtcttggg ccacagctca
     1441 tccaccacca cgtcgacatc gtgcttgatg tcggtcaggc gcaggatgcc caccgcgagc
     1501 agcgtcccca gcagcaccag cacgaggccg aagcccagcc ccagcttcat acccaccttc
     1561 atgttcctga acattcgctt tcccctgttt ccggtttctt ctgcagcatt catgcagcaa
     1621 ggaagcgcgc ccgcggcgca cccccggtgt cgcggccctc gtgcgcgatc gcacgctgga
     1681 ccagttgtgg cacgtccagg atcagcgcca cctcgccgct gcccaggatg gtggagccgg
     1741 cgatgccggc gacgcgctcg aagagcggcc cgagcggctt gatcaccgcc tggcattcgc
     1801 cgagcagatg gtccaccgcg atccccgcct gccgcccgcc gaagcgcacc acgaccacgt
     1861 tctgccgcgg cggcggtgcg ccctcgagcg cgaacagggc gcgcaggcgc aggaagggca
     1921 ggacctggcc gcgcaggtcg aggtagccgg cggcgccggc gtcggccggc agctcgatgc
     1981 actcgaccac cgcgtcgagc ggcaggatga aggtggcgcc ggcgacaccg acctggaagc
     2041 cgtcgatgat cgccagcgtg agcggcaggc acagccgcat cgtggtgccc tgccccggcg
     2101 cgctgtggat gtcgagggtg ccgcgcagcg catcgacgct gctcttgacc acgtccatgc
     2161 cgacgccgcg gccggacagg ctggtgagtt cggccgcggt ggagaagccc ggcgccagga
     2221 cgaggcggtg gatctcggcc tcggagagca cggcgtccgc cgcgaccagg ccgcgctcga
     2281 cggccttggc gaggatgcgc cgcgcgtcca gcccgccgcc gtcgtcggcg acctcgatga
     2341 cgatgctgcc cgactcgtgg aaggcgcgca gctcgacccg gccgcgcgcc ggcttgcccg
     2401 ccgcggcgcg gcgttcgggc gattcgatgc cgtggtcgag ggcgttgcgc acgaggtgca
     2461 tgagcgggtc gccgatcttc tcgaccatgg acttgtcgag ctcggcctcg gcgccgcgga
     2521 tcaccagctc gatgtccttg ccgagctcgc gcgcgaggtc gcgcaccacg cgcgggaagc
     2581 ggttgaagac ctcgccgatc ggggtcatgc gcaggcgcag cgcggcgtcg cgcacgtcct
     2641 cgaccaggcg cagcaggttg gcggcggtct cctgcagcgc gcggctgccg gcctgctgcg
     2701 cctgcagctg ggtggcggcg ccggcgatga cgagctcgcc gacctggtcg atgagcgcgt
     2761 cgagccggtc ggcgggcacc ttgacgctgc gcgtctcgct ggcgcggcgc tcctcggcgg
     2821 ccttctgctt gccgagcgcg gcctgcacca cctgggcggg cacaccggcg tgctcgacca
     2881 gtagctcgcc cacgcgcggc gcctcggcct gcacgtgctg ggcggcgagc gcggcctcga
     2941 gctcgcgcgc ggtgaccgag ccgcaggtca cgaggatctc gccgagtcgg cgcgggtcct
     3001 cgtcgagcgc ctcgatgagg gcgaggtact cctcgacgcg cgccgccggc ggcaccagca
     3061 cgatgcgcgc gctgtcctgc acgaactcga acaccgcctc gatctcggcg cggggggccg
     3121 tgctgcgcag cgcgatctcg aagccgaggt agcagttttc cgggtcgccg gcggcgagct
     3181 cgggcagggt gtcggcgatg gtctcgacgt gcacgagctc gccgagggtg cgcaggtagt
     3241 ggatgaaggc gagcgggtcc atgccgttcc tgagcacctc gggcgcgaag cgcagcgaca
     3301 ggtgccagtg ctcggcgccg agcgccacgc tgccgccgac ctcgagcgcg ggcgcgacct
     3361 cggtcgcgga gcagacgatc gggtccccct ccgccacggt cgccggcagt gcggcggggg
     3421 tggcgggcgt cgtggcgcag aaggcttcga ggcgggcgcg cagcggcgcg ccctcgaact
     3481 cgggcggagg aagttcggcg gcgaccgcgg cgacgaggct ggcgatgtgg tcctgcgact
     3541 cgagcagcag gttgatcagg gcgtcggaga gttcgagttc gccgttgcgc atgcggtcga
     3601 gcacgctctc gacggcgtgg gtgaagtgca ccaccgcgtc cagcccgaac aggccggccg
     3661 agcccttgat ggtgtgggcg caacggaaca gcgcgtgcag ctcgtcggtg ggcagcgcgg
     3721 cggtctcggc gcgcaggagg atctcctcca tctgcgcgag cagctcgcgg ctctcgtcga
     3781 ggaaggtggc cagggcctgg gagaggtcca tcagcgtgcc tcctgcgtgg ccggcgcggc
     3841 gtcctcgagc agcgggtcgc cgagcgcgcc gccgaggcgg gcgaggtcga gcaggcgcag
     3901 caccgcggcg gagtggttga cgaagcgcac gctgcgtccg gcgctgcgct tggccatcag
     3961 catgagctgc aggccggcgg tgtcgatctc gtcgatgcgg ccgaggtcga tctcgagcgc
     4021 ttcgtgggtg gcgagcgcct gcagccaggc ctcgcgcagc gcggccacgg tgtagatgcc
     4081 gagctcgccc tcgggggcga ggcgggcgac gccagccgcg ggggtgtcga gtgcaggggt
     4141 gtccatgggc ggcgcgctca catcaccagc ttggacaccg ccgccagcat ctgctgcggc
     4201 tggaagggct tgaccaccca ggcgcgcgcg ccggcttcct ggccgaggcg cttcttctcc
     4261 tcggcggcct cggtggtgag catgatcacc ggggtgaact tgtagctcgg gttcttcttg
     4321 acctcgcgca cgaaggcgat gccgtccatg ttgggcatgt tcacgtcgga gatgatgaga
     4381 tgaaccttct ggccgccgag cttgccaagg gcgtcgcggc cgtcgcaggc ctcgatcacc
     4441 tggtagccgg cgcccttgag cgacaggctc accacctggc gcagcgaggc ggagtcgtcg
     4501 acgatcagca cggtcttgga catgggggaa tttctcctgc tcaaaagaag gtgatgtcgc
     4561 cgccagccgg ggcggggttc gcgtcggcgc ggcgggcgtg ttgttcgagg gtggtgtagg
     4621 tgcgctcgag cgcggcgatc caggcggcga cgtcgatcgg cgccggcacc cgcccgccgg
     4681 cgaggcgggc ctcgtcttcg gcgacctggg cggcgaggcg cgccatgtcg gtgcgcaccg
     4741 cctcgagaat ctggctgacg cggtcctgga actggagctg caccagcacg ccctcgatgc
     4801 gggactgcac cgcggtgctg tcttcctcga gcgcgcgcgc ggcctgcgcc atggcgttgc
     4861 cgacctggtc gaagcggccg accacgctgc cgatcgccgc ctcggagtcg cgcaccagga
     4921 cggcgtcgcc gcgggccatg tgctcggccg ctgcgagcgc ctcggccatc gcggcgtggg
     4981 cgctctcgac cttggcgcgg atacgctcgc cggtggcgcc ggagaggtcg gagagcttgc
     5041 gcacctcgtc ggcgaccacc gcgaagccgc gccccacctc gccggcgcgg gcggcctcga
     5101 tcgccgcgtt gagggcaagc aggttggtct gcttggcgat ctcgccgacc tcgctcgcca
     5161 tgcgcatcag ctcctcggtg acctgggcga ggcgggcgac cgcctgcgac aggctctgct
     5221 tctcggcgag cgcggcgttg agcccggcga gcacgcggtc gagctcgccg cgggcatggc
     5281 cgatcacccc gaccacgctg tcctcgccgc ggtcggcggt ggactggcgc gagcgcagca
     5341 gcgcgtcctg caggcgctcg aggatgtgcg cgaactcgcc ggcgagcgcg cccacggcgg
     5401 tctcggtctg gctgcgcgag atgcccacgt ggtcgtgcca gcgcgccagc gcggcctgcg
     5461 ccaactgctg caggctggcg aggtaggcct cgcgctcggc caggcgcgca cgctccgccg
     5521 tggccacgcg cgccgcgacg tcagcctggc gggcgcgctc gcgacgcagt atccaccatg
     5581 gcagg
//
LOCUS       DAOQNN010000208         5548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8607_length_5548_cov_11.231932, whole genome shotgun sequence.
ACCESSION   DAOQNN010000208 DAOQNN010000000
VERSION     DAOQNN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8607_length_5548_cov_11.231932"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..754)
                     /locus_tag="PKV97_11015"
     CDS             complement(131..754)
                     /locus_tag="PKV97_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06450.1"
                     /translation="MTFVQLPTTPPDITGDLGRAWRTDLRAILDKARAKYGKAPPLEL
                     NIAGWIAYAPYSHPLWPCVTVGCISLHDVPGWPPAVIHLPGATHEVFVAAIDPAYALR
                     IDEQPPTLRPLNFAGQFIAGSHDEARERIEQAVRDICDGTLNPDSDNRHAWAERFSRS
                     NFKAGADLPDVIVPTPDGGTLVVGTGATNVRALQQVVGDGDDDGKRH"
     gene            complement(751..1047)
                     /locus_tag="PKV97_11020"
     CDS             complement(751..1047)
                     /locus_tag="PKV97_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06451.1"
                     /translation="MNESCTRLQHANLTALHRAVCDITQAVVAERSGLSPTLVSRLLS
                     GKDSEDEAGGAVHRVLAILAACNLTVVPQSYRHVDPDEYRAMQILASKRLREDV"
     gene            complement(1178..1819)
                     /locus_tag="PKV97_11025"
     CDS             complement(1178..1819)
                     /locus_tag="PKV97_11025"
                     /inference="COORDINATES: protein motif:HMM:NF027273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HNB06452.1"
                     /translation="MRYAPGRLGDFNLAVRFQQHLVMDLKAWIDLERGRQTKLAAEIG
                     AQPQLVWQWAHGKRPIPADRRPDIERATGGAVTCEEMTSDEVVWSRISDAHWKWHPLG
                     RPVRDLTREARLMDALMLKRRCAFCGKRGGDPVGCAVEILGGQSKLAAAIGVRQQHVW
                     NWLNRDTVPPEFCAAIERATEGKVTRRDLRPSDWWRIWPELVTEEHPIPVEAA"
     gene            complement(1851..2729)
                     /locus_tag="PKV97_11030"
     CDS             complement(1851..2729)
                     /locus_tag="PKV97_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNB06453.1"
                     /translation="MNCIEQVVTDQYALYHGDCVEVLRGLPDHSVGYSIFSPPFVSLY
                     TYSNSPRDMGNVRDEAEFFQHFDFLIAELRRVMKPGRNVSFHCMLMPSSKERDGVIGL
                     KDFRGDLIRSFQRHGFIYHSEVCIWKDPVTSMQRTKALGLLHKTVRENAAMSRQGIPD
                     YLVTMRAPGECERVTHDPAEYPVDLWQRVASPVWMDINPSDTLQYMSAREHDDERHIA
                     PLQLEVIRRGVMLWTNPGDIVLSPFGGIGSEPYVAVEMGRKAVAVELKASYFRQMVQN
                     VAAAKAKTADLFSEAA"
     gene            complement(2726..4108)
                     /locus_tag="PKV97_11035"
     CDS             complement(2726..4108)
                     /locus_tag="PKV97_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06454.1"
                     /translation="MNDYTAYVDRKLSRVPPTGIASGFIVPGDMFPHQSALTAWAIRR
                     GRAAIFADTGLGKMRMELAFADAVRRHTKRPVMIHTPLAVAAQLRAEGLRIGVEAAIC
                     REVSDVTDGINIANYERLHKFDPSVFGGVVLDESGCIKHHDTKTFQQLTAAYRDTPFK
                     LPATATPAPNDWTELGTHAEFLGICTRQEMLAEFFCHDGGDTSVWRLKGHARDVFWRW
                     VASWGAMIRRPSDLGFDDSAYALPALHVHEHHVDYELPLAGMLFATEAESLSERREAR
                     RASMEDRVKACAAIVNDDRQPWVVWCDLNAEGDALTAAIDDAIQIAGSDSVDVKEQRL
                     ADFSEGRARVLVSKPSICGWGLNWQHAARMAFVGVTDSYEAYYQAVRRCWRFGQTRDV
                     HVHVFASKAEGAVVANLRRKEHEAGRMAESLSAETRDAVMAEVTGMQRQTNPYNATHR
                     VQVPAFLEAA"
     gene            4489..4632
                     /locus_tag="PKV97_11040"
     CDS             4489..4632
                     /locus_tag="PKV97_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06455.1"
                     /translation="MPQTTSFDLAAAWTAAMTQARADLPGAHDDDIFDIVSATLAVTL
                     GGA"
     gene            4632..5048
                     /locus_tag="PKV97_11045"
     CDS             4632..5048
                     /locus_tag="PKV97_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06456.1"
                     /translation="MSHVTDQSRALSHYVPLAREAQLHAQVERLHVELCAARQELRLL
                     KSEDPAQLVRWSTLPGILRGLGMPQATEDLQALLGALSHVVVESGMDDPEGCVTHLDK
                     ASDAAEALRVLDGDEMALAHREYVREMEDDDRRAGY"
     gene            5050..>5548
                     /locus_tag="PKV97_11050"
     CDS             5050..>5548
                     /locus_tag="PKV97_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06457.1"
                     /translation="MSEKKKTAAEPVESAETITAYKGFGADWTCRGYQYAVGKTYAHE
                     GAVQACESGFHACEYPLHVLRYYRPGTSRFAVVEQSGQIARHGDDSKVASSRIAIKAE
                     IDIAGLIKAAVEYTVKRSKPEPGATTDKEHGAVSASGNFGAARRAATPARPRRAATPA
                     RPRRAA"
BASE COUNT          913 a         1872 c         1864 g          899 t
ORIGIN      
        1 ggcagcccct tggtgtccag gtgcagcgcg tacgcgatct ggctggagtt gaactccttc
       61 gtgcggccct gcgcctcgag ccaggagacg atgcgatagg ccgcggtgcc gggctgcggt
      121 gcgtaggcca tcagtgccgc ttcccgtcgt cgtcaccatc gcccacgacc tgctgcagcg
      181 cgcgcacgtt cgtggcccca gttcccacga cgagcgtgcc gccgtcaggc gtgggaacaa
      241 tgacgtcggg caggtcagcc ccggccttga agttggagcg actgaagcgc tcggcccatg
      301 catggcggtt gtcgctgtcg gggttcagcg tgccgtcgca gatgtcccgc acggcctgct
      361 cgatgcgctc gcgcgcctcg tcgtggctgc cggcgatgaa ctggccggcg aagttcagcg
      421 gccgcagggt cggcggctgc tcgtcgatgc gaagcgcata cgccgggtcg atcgccgcga
      481 cgaacacctc gtgcgtggcg cccggcaggt ggataactgc gggcggccag ccgggcacgt
      541 cgtgcagcga gatgcagccg acggtgacgc acggccacag cgggtgcgag tacggcgcgt
      601 acgcgatcca gccggcgatg ttcagttcaa gcggcggcgc cttgccgtac ttcgcccgag
      661 ccttgtcgag gatcgcgcgc aggtcggtgc gccaggcgcg acccaggtcg ccagtgatgt
      721 cgggcggcgt ggtgggaagt tggacgaacg tcacacatcc tcccgcagcc gcttcgacgc
      781 caggatctgc atcgcccggt actcgtcggg gtcgacgtgc cggtacgact gcggcacgac
      841 ggtgagattg caggccgcca ggatcgccag cacgcggtgc acggcgccgc cggcctcgtc
      901 ctcgctgtcc ttgcccgaga gcagccggct caccagcgtg ggcgacaggc ccgaccgctc
      961 ggccaccacc gcctgggtga tgtcgcacac cgcgcgatgc agcgcggtca ggttcgcgtg
     1021 ctgcagccgc gtgcaggatt cgttcatcgc tgtgaaccct gcgaggtagg ggggcagcgc
     1081 attgcatgca agcatgcaag tcggcgcggc atggccgggc gggcgcgcaa ggcgagcggc
     1141 ggccacagtc cggcccgtga gcagcagttc cgctccatca ggcagcctcg acggggatcg
     1201 ggtgctcttc ggtgaccagc tcgggccaga tgcgccacca gtcgctgggg cgcaggtcgc
     1261 gtcgagtgac cttgccttcg gtcgcgcgct caatcgccgc gcagaactcg ggcggcaccg
     1321 tgtctcggtt cagccagttc cacacgtgct gctgacgcac accgatggct gcggccagct
     1381 tgctctggcc gccgaggatc tccaccgcac acccgaccgg atcgccgccg cgcttcccac
     1441 agaacgcgca tcgccgcttc aacatgaggg cgtccatcag gcgggcctcg cgcgtcagat
     1501 cgcggaccgg cctgccgagc gggtgccact tccaatgggc gtcgctgatg cgcgaccaga
     1561 ccacctcgtc ggaggtcatc tcctcgcacg tcactgcgcc gccggtggca cgctcgatgt
     1621 cgggccggcg atcagcgggg atcggccgct tgccatgagc ccactgccag accagttgcg
     1681 gttgcgcgcc gatttcggcc gcgagcttgg tctggcgccc gcgctccaga tcgatccatg
     1741 ccttgaggtc catcaccagg tgctgctgaa aacgcacagc caggttgaag tcgccaaggc
     1801 ggccgggcgc atagcgcaca ggcgagagag cgattggccg gggatgccgg tcatgcggcc
     1861 tcagaaaaca gatcagccgt cttcgccttc gccgcggcca cgttctgcac catctgacgg
     1921 aagtagctcg ccttcaattc gacggcaacg gccttgcgac ccatctcgac cgcgacatac
     1981 ggctcggagc cgatgccgcc aaacggggac agaacgatgt cgccgggatt cgtccagagc
     2041 atgacgccgc ggcgaatcac ctccagttgc agcggggcga tgtgccgttc gtcgtcgtgc
     2101 tcgcgggcac tcatgtactg cagcgtgtcg gacggattga tgtccatcca caccggcgac
     2161 gccacgcgct gccacaggtc aaccgggtac tcggccgggt cgtgcgtgac gcgctcgcac
     2221 tcgccaggtg ctcgcatggt cacgaggtag tcaggtatgc cttgccggct catcgcggcg
     2281 ttttcgcgga ccgtcttgtg cagcaggccg agcgccttcg tgcgctgcat gctcgtcacc
     2341 gggtctttcc agatgcagac ctcggagtga tagatgaacc cgtgccgctg aaaggagcgg
     2401 atcagatcgc cgcgaaagtc cttcaggccg atgactccat cgcgctcctt gctgcttggc
     2461 atcagcatgc agtggaacga gacgttgcgg cccggcttca tcacgcggcg cagctcggcg
     2521 atcaggaagt cgaagtgctg gaagaactcg gcctcgtcgc ggacgtttcc catgtcgcgc
     2581 gggctgttcg agtaggtgta gagcgacacg aacggcggcg agaagatcga atagccgaca
     2641 gagtgatccg gcaggccgcg cagcacctcg acgcagtcgc cgtggtagag cgcgtattga
     2701 tcggtgacga cttgctcaat gcagttcatg cggcctccag gaatgccggc acctgcactc
     2761 ggtgcgtggc gttgtatgga ttggtctgcc gttgcatgcc ggtcacttcg gccatcacgg
     2821 catcgcgggt ctcggcactg aggctctctg ccatgcggcc agcctcatgc tccttgcggc
     2881 gcaggttcgc caccacggcg ccctcggcct tgctggcgaa aacgtgcaca tgcacgtcgc
     2941 gcgtctgccc aaagcgccag caccgtcgaa cggcctggta atacgcctcg tagctgtcgg
     3001 tgacgccgac gaacgccata cgggcggcgt gctgccagtt gaggccccag ccgcagatgg
     3061 atggtttgct caccagcacg cgcgcccgcc cttcggagaa gtccgcgagg cgctgttcct
     3121 tcacgtccac cgaatcggag ccggcgatct ggatggcgtc gtcgatcgct gcggtcagcg
     3181 cgtcgccctc ggcgttcaag tcgcaccaca cgacccacgg ctgccggtca tcgttgacga
     3241 tggccgcgca cgccttcacg cggtcctcca tcgaagccct gcgggcctcg cggcgctcgc
     3301 tgagggactc cgcctcggta gcgaacagca tcccggccag gggcagttcg tagtcgacgt
     3361 gatgctcgtg cacgtgcagc gcaggaagcg catacgccga gtcgtcgaag cccaggtcag
     3421 agggccggcg aatcatcgcg ccccacgatg cgacccaacg ccagaacaca tcccgcgcgt
     3481 ggcccttcag gcgccatacg ctcgtgtccc cgccgtcgtg gcagaagaac tcggccagca
     3541 tctcctgccg cgtgcagatg cccaggaact cggcgtgcgt gcccagctcc gtccagtcgt
     3601 tcggcgctgg cgtcgcggta gccggcaact tgaacggcgt atcgcggtag gccgcggtca
     3661 actgctggaa ggtctttgtg tcgtgatgct tgatgcatcc ggactcgtcg agcacgactc
     3721 cgccgaacac ggacgggtcg aacttgtgca gccgctcgta gttggcgatg ttgatgccgt
     3781 ccgtcacgtc ggagacttcg cggcagatcg cggcctcgac gccgatgcgc aggccctcgg
     3841 cgcgcaattg cgcggccaca gcgagcggcg tatggatcat cactggccgc ttggtgtggc
     3901 ggcgcactgc gtcggcgaac gcgagttcca tccgcatctt gccgaggccc gtgtccgcaa
     3961 agatcgccgc acgcccgcga cggatggccc acgcggtcag cgcagactga tgcggaaaca
     4021 tgtcgcccgg cacgatgaat ccagatgcga tgccagtcgg cggcacgcgc gatagcttgc
     4081 ggtcgacgta tgcggtgtag tcgttcacgc cacccccctc cgagtgctgt acgcccgcac
     4141 acccgccacg tttggcaggc ttgcgggtgc tgctgagtgc ggctccggga tcatctcttc
     4201 gagagtcacc tgcccgccgg tgtagtcccg aaccgccttc atgcgatcga ccgggatgcc
     4261 ggtcgtgcgc cactgagaca cggctgccac agagatgcca aagtgtgcgg ccatcgcgga
     4321 catgcgcccg cgctccgcgt cgatccatgt gtaaaggtcc atgaaggccg acgttaagtc
     4381 aaacagtggc agttagtcaa gcgaacgcct caaaatattt taagcaaaac ctattgacag
     4441 gcatcaggcg cccgcttaac gttgcggcca tcccacccgg agagccgcat gccccagacg
     4501 accagtttcg acctcgccgc cgcctggacc gcggcgatga cccaggcccg cgccgatctg
     4561 ccaggcgcgc atgacgacga catcttcgac atcgtgtcgg ccacgctcgc ggtgacgctg
     4621 gggggtgcgt gatgagccac gtcaccgacc aatcccgcgc cctgtcccac tacgtaccgc
     4681 tcgcccgcga ggcccagctg cacgcccagg tcgagcgcct gcacgtcgag ctctgcgcgg
     4741 cccgccagga gctgcgcctg ctgaagtccg aagacccggc gcagctggtc cgctggtcga
     4801 ccctgccggg catcttgcgc ggcctcggca tgccgcaggc gacggaagac ctgcaggcgc
     4861 tgctcggtgc gctgtcgcac gtcgtcgtcg agtcgggcat ggacgacccc gagggctgcg
     4921 tcacgcatct cgacaaggcc agcgacgcgg ccgaggcgct gcgggtgctg gacggcgacg
     4981 agatggcgct cgcgcatcgg gagtacgtgc gcgagatgga ggatgacgat cggcgggcgg
     5041 ggtattgaga tgagcgagaa gaagaagact gccgcggagc cagtcgagtc cgcggagacc
     5101 atcaccgcct acaaaggctt cggcgccgac tggacctgcc gcggctatca gtacgcggtc
     5161 ggcaagacgt acgcgcacga aggcgctgtg caagcctgcg agtcgggctt ccacgcctgc
     5221 gagtacccgc tgcacgtact gcgctactac cggcccggca cgtcgcgctt tgccgtcgtc
     5281 gagcagtcgg ggcagatcgc tcgacacggc gacgactcga aggtcgccag ctcgcgcatc
     5341 gccatcaagg ccgaaattga catcgcagga ctcatcaagg cagcggtcga gtacacggtc
     5401 aagcgcagca agccggagcc tggcgcgacg accgacaagg agcatggggc tgtgtccgcc
     5461 agcggcaact tcggcgcggc acggcgagcg gctactccgg cgcggccacg gcgagcggct
     5521 actccggcgc ggccacggcg agcggcaa
//
LOCUS       DAOQNN010000209         5528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8651_length_5528_cov_9.821122, whole genome shotgun sequence.
ACCESSION   DAOQNN010000209 DAOQNN010000000
VERSION     DAOQNN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8651_length_5528_cov_9.821122"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PKV97_11055"
     CDS             <1..922
                     /locus_tag="PKV97_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNB06458.1"
                     /translation="LGWAEDPAQDGAATDAADDAPRARLQHLRFATPDGEQTVEADAL
                     VLALGGGSWAKLGSDGAWVAPLRAAGVEVADLLPANCGFDVVGPAGTGWSAHFLDHFR
                     GQPLKSVAARCTDPAGRVHARAGECLISATGIEGGLVYALSAPLRDTLLARGEAVLEL
                     DLAPGRSLERLAAELARPRGSRSFSSHLHSQAGIKGVKAGLLRECLAKAEFDDPARLA
                     AAIKAMPLRLVATRPLDEAISSAGGVRFEALDAQLMLRARPGVFVAGEMLDWEAPTGG
                     YLLTACFATGLAAGTAAADWLSQQPRPPCP"
     gene            972..1604
                     /locus_tag="PKV97_11060"
     CDS             972..1604
                     /locus_tag="PKV97_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNB06459.1"
                     /translation="MNLHPFLVAIEVGGTLAFAMSGLIEATRKRMDIVGVFSVAFVSA
                     FGGGTLRDILLDRRPLFWIENQEYLWLVLAMVATTPLWLQTLRHKVGGRLMELADALG
                     LGLFAISGATLADAAGMPVLVSMMMGAITAVFGGVSRDVLCNEVPKVYSDHRPYAMCA
                     LFGCGVFLALDAIGLPLPIATLAGIAAATGSRLLALVFDVRLPAWPAHRD"
     gene            complement(1635..2837)
                     /locus_tag="PKV97_11065"
     CDS             complement(1635..2837)
                     /locus_tag="PKV97_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNB06460.1"
                     /translation="MQPVQTSNDVLFILLGAIMVLAMHAGFAFLEVGTVRRKNQVNAL
                     VKILSDFAVSTLAYFFIGYGIAYGVHFFSGAGALVEHHGFELTRFFFLLTFAAAIPAI
                     ISGGIAERARFQPQLAATFLLVGFVYPFFEGIAWNQAFGIQAWLKASFGAEFHDFAGS
                     VVVHAMGGWIALPAVLLLGARRGRYARNGQISAHPPSSIPFLALGAWILIVGWFGFNV
                     MSAQRLENISGLVAINSLMAMVGGTVAALAAGRNDPGFVHNGPLAGLVAVCAGSDVMH
                     PLGALVVGAVAGGLFVVMFTLTQNRWKIDDVLGVWPLHGLCGAWGGLAAGIFGSQALG
                     GLGGVAFASQAIMTALGIAVALAGGVLVYGGLKLAVGLRLDHEEEFNGADLSIHRITA
                     TAERESNW"
     gene            3155..4354
                     /locus_tag="PKV97_11070"
     CDS             3155..4354
                     /locus_tag="PKV97_11070"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNB06461.1"
                     /translation="MFTAVSAVCVTGLVTVDTGTYWSSFGQWVIMALFQIGGFGMMTA
                     ATLLGLLVNRSFRLRTRLTAQAETHTLGIGDVSSVAKLVLFVTVIVEVMTALALALRL
                     HLGYDLPLAEAAWSGLFHSVSAFNNAGFSIHADGLIRYASDSYLLAPIMLAIVIGGFG
                     FPVLQDLRNKLRDPQHWSVHTKLTLVGTAVLLAGGAILLLAFEWANPRTLGSMPAGDK
                     LLSAIFASVSARTAGFNSIDIGGLTHESLGLHYFLMFIGGGSAGTAGGVKVGTAMILV
                     MLVIAEIRGRMDTEAFGRRISHSAQRQAITVLVLSSAVVVLGTVFILHTTEIATDRVI
                     FEVISAFATVGLSTGITADLPDSAQLMLILLMYIGRVGTITLAASLALGEHRMPFRYP
                     EEHPVVG"
     gene            4347..5042
                     /locus_tag="PKV97_11075"
     CDS             4347..5042
                     /locus_tag="PKV97_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNB06462.1"
                     /translation="MARLFTEQFAFSKGDSVVVIGLGRFGGAVANSLMQLGHDVMGID
                     RDAEPVHEWADLLTHAVQADSTSAMTMRQLGVGDFAHAIVGIGGDLAASLMTVMALTE
                     LEIPDIWVKAMTPEHGKIAERIGAHHVVYPEADMGERVAHLISGRMMDYIEFDDGFAI
                     AKIQAPSVTHERSLAESAVREHFGVTVVGIKRAHEDFQHGKPGSVVLPGDLLIVSGPT
                     KKVEAFAASGRRR"
     gene            5123..5344
                     /locus_tag="PKV97_11080"
     CDS             5123..5344
                     /locus_tag="PKV97_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06463.1"
                     /translation="MLNWILNQFKRQSAEDLERAREMVKAAERGHSHPDLGKARDVAR
                     TLGIEVDVHASAEQLIQAIRRYLARHGEM"
BASE COUNT          859 a         1969 c         1836 g          864 t
ORIGIN      
        1 gctgggctgg gcggaggatc ccgcgcagga tggtgcggcc accgacgccg ccgatgacgc
       61 gccccgcgcg cgcctgcagc acctgcgctt cgccaccccg gacggcgagc agacggtcga
      121 ggccgacgcg ctcgtgctcg cgctcggcgg cggcagctgg gcgaagctcg gttccgatgg
      181 cgcctgggtg gcaccgctgc gcgccgccgg ggtcgaggtc gccgacctgc tcccggccaa
      241 ctgcggtttc gacgtcgtcg gccccgccgg cacgggctgg agcgcgcact tcctcgatca
      301 cttccgcggc cagccgctca agtccgtcgc cgcacgctgc accgaccccg ccggccgcgt
      361 ccatgcgcgc gccggcgaat gcctgatctc cgccaccggc atcgaaggcg gcctggtcta
      421 tgcgctctcc gcgccgctgc gcgacaccct gctcgcgcgc ggcgaggccg tgctcgagct
      481 cgacctcgcc cccggccgca gcctcgagcg cctcgccgcc gagctcgccc gcccgcgcgg
      541 cagccgctcg ttttccagcc acctgcacag ccaggccggc atcaagggcg tcaaggccgg
      601 cctgctgcgc gaatgcctgg cgaaggccga gttcgacgat cccgcccgcc tcgccgcggc
      661 catcaaggcc atgccgctgc gcctcgtcgc cacgcgcccg ctcgacgagg ccatcagcag
      721 cgccggcggc gtgcgcttcg aggcgctgga tgcgcagctg atgctgcgcg cccgccccgg
      781 cgtcttcgtc gccggcgaga tgctcgactg ggaagcgcct accggtggct acctgctcac
      841 cgcctgcttc gccaccggcc tcgccgccgg aaccgccgcc gccgactggc tgtcgcagca
      901 accccgaccg ccctgcccct gacgcctcac tccttccgcc tcacgcctca ctcttcacgc
      961 ctcgcacccc aatgaacctc cacccattcc tggtcgcaat cgaagtcggc ggcaccctgg
     1021 ccttcgcgat gtccggcctc atcgaggcca cccgcaagcg catggacatc gtcggcgtgt
     1081 tctcggtcgc cttcgtcagc gccttcggcg gcggcaccct gcgcgacatc ctgctcgacc
     1141 gccgcccgct gttctggatc gagaaccagg aatacctctg gctggtgctg gccatggtgg
     1201 cgacgacgcc gctatggctg cagaccctgc gccacaaggt cggcggccgc ctgatggagc
     1261 tcgccgacgc gctcggcctc ggcctcttcg ccatctccgg cgccactctc gccgacgccg
     1321 ccggcatgcc ggtgctggtg tcgatgatga tgggggcgat caccgcggtg ttcggcggcg
     1381 tgtcgcgcga cgtgctgtgc aacgaggtgc ccaaggtcta tagcgaccac cgcccctacg
     1441 ccatgtgcgc gctgttcggc tgcggcgtct tcctcgcgct cgacgcgatc ggcctgccgc
     1501 tgccgatcgc caccctggcc ggcatcgccg ccgctaccgg ctcccgcctg ctcgcgctcg
     1561 tcttcgacgt gcgcctgccg gcctggccgg cgcatcggga ctgaacgcgg ggtcggtgaa
     1621 cgaccgcctg ccagttacca gttcgactcg cgctcggccg tcgcggtgat gcggtggatc
     1681 gacaggtcgg cgccgttgaa ctcttcctcg tggtccaggc gcaggcccac cgcgagcttg
     1741 agcccgccat agacgagcac cccgccggcg agcgcgacgg cgatcccgag cgcggtcatg
     1801 atcgcctgcg aggcgaacgc caccccgccc agcccgccca gcgcctggct gccgaagatg
     1861 cccgccgcca gcccgcccca ggcgccgcac aggccgtgca gcggccacac cccgagcacg
     1921 tcgtcgatct tccagcggtt ctgcgtcagc gtgaacatca ccacgaacag tccccccgcc
     1981 accgcgccga ccaccagcgc accgagcggg tgcatcacgt ccgaacccgc gcacaccgcc
     2041 accagcccgg cgagcggccc gttgtgcacg aagcccgggt cgttgcgccc ggccgcgagc
     2101 gccgccaccg tgccgccgac catcgccatc agcgagttga tcgccaccag cccggagatg
     2161 ttctccaggc gctgcgccga catcacgttg aagccgaacc agcccacgat caggatccac
     2221 gcgccgagcg cgaggaaggg gatcgacgag ggcgggtggg ccgagatctg cccgttgcgc
     2281 gcatagcgcc cgcgccgcgc gcccagcagc agcaccgccg gcagcgcgat ccagccgccc
     2341 atcgcatgca ccaccaccga gccggcgaag tcgtggaact ccgcgccgaa gctcgccttg
     2401 agccaggcct ggatgccgaa ggcctggttc cacgcgatgc cctcgaagaa gggatagacg
     2461 aagccgacga gcaggaaggt cgccgccagc tgcggctgga agcgcgcgcg ctcggcgatg
     2521 ccgcccgaga tgatggcggg gatcgccgcc gcgaaggtga gcaggaagaa gaagcgcgtg
     2581 agctcgaagc cgtggtgctc caccagcgcg ccggcgccgg agaagaaatg cacgccgtag
     2641 gcgatgccgt agccgatgaa gaaataggcc agcgtcgaca ccgcgaagtc ggacaggatc
     2701 ttgaccagcg cgttgacctg gttcttccgc cgcaccgtcc ccacctccag aaaggcgaag
     2761 ccggcgtgca tggcgagcac catgatggcg cccagcagga tgaacagcac gtcgttgctc
     2821 gtttgaacgg gttgcatgat gcctccgaaa gtgacgggca tccttagcaa gcaccgttcc
     2881 agtgcgatga atcgggcgga atccaatgcg tcacgccatg atggtgcgca gaagacgcac
     2941 cagaggggtg cattcgtcag gcagcgcgca ccgtggcggg gcgcggtgag gagctcgtca
     3001 tcgcgcgtgc cggccaagcc gtcgcagcgc gggccgcctg ccgtcccgta cgccgcaggg
     3061 ccgccggctc gtcgaagaac cgcaggtgtc gagagccgac atgtccggcc aggcgggcgt
     3121 atgatctgca gccgacaagc gccagttcgc ggccctgttc accgcggtgt cggccgtgtg
     3181 cgtgaccggc ctggtgacgg tggacaccgg cacctactgg tcgtcgttcg ggcagtgggt
     3241 gatcatggcg ctgttccaga tcggcggctt cgggatgatg accgccgcca cgctgctcgg
     3301 gctgctggtc aatcgctcct ttcgcctgcg cacacgactc acggcgcagg cggaaaccca
     3361 cacactcggg atcggcgacg tcagcagcgt cgccaagctc gtgctgttcg tgaccgtgat
     3421 cgtcgaggtg atgaccgcac tggcgctggc tctgcgtttg catctcggtt acgacctgcc
     3481 tttggccgag gctgcctgga gcggcctgtt ccattccgtc tcggccttca acaacgccgg
     3541 cttttcgatc cacgccgatg gcttgatccg ctacgcgtcc gacagctacc tcctcgcacc
     3601 catcatgctg gcgatcgtga tcggcggttt cggctttccg gtgctgcaag acctccgcaa
     3661 caagctcagg gacccccagc attggtccgt gcacaccaag ctgacactcg tgggaacggc
     3721 cgtgctgctg gctggcggcg cgatcctgct gcttgccttc gagtgggcga acccgcgcac
     3781 cctcggatcg atgccggcag gggacaagct gttgtcggcg atcttcgcgt cggtgtcggc
     3841 acgcactgcc ggattcaact cgatcgatat cggtgggctg acgcacgaga gtctcggcct
     3901 gcactacttc ctgatgttca tcggcggcgg cagtgccggc acggccggtg gcgtgaaggt
     3961 gggcacggcg atgatcctgg tgatgctggt catcgccgag atccgcggcc ggatggacac
     4021 cgaggccttc ggccggcgca tcagccattc ggcccagcgc caggccatca ccgtgctggt
     4081 cctcagcagt gcggtcgtcg tgctgggcac ggtgttcatc ctgcacacca ccgagatcgc
     4141 taccgaccgg gtcatcttcg aggtgatctc ggccttcgcg acggtcggcc tgtccaccgg
     4201 catcactgca gacctgccgg attccgccca gctgatgctg atcctgctga tgtacatcgg
     4261 ccgggtcggc accatcacac tggcggcgtc gctcgcgctc ggcgagcacc gcatgccctt
     4321 ccgctatcca gaggagcatc ccgttgttgg ctaggttatt tacagagcag ttcgccttct
     4381 cgaaaggcga cagtgtggtc gtgatcggcc tcgggcgctt cggcggagcc gtcgccaatt
     4441 cgctgatgca gctcgggcac gacgtgatgg gcatcgaccg cgatgcggag ccggtacacg
     4501 aatgggcgga tctgctcacc catgcggtgc aggcggactc gaccagtgcg atgaccatgc
     4561 gccagcttgg cgtgggcgac ttcgcgcacg ccatcgtggg gatcggcggc gatctggccg
     4621 cgagcctgat gacggtgatg gcgctgaccg aactggagat cccggacatc tgggtcaagg
     4681 ccatgacgcc cgagcacggc aagatcgccg agcgcatcgg cgcacatcac gtggtctacc
     4741 cggaggccga catgggcgag cgcgtcgcgc atctcatcag cggccgcatg atggactaca
     4801 ttgaattcga cgacggcttt gcgatcgcga agatccaggc gccgagcgtc acgcacgagc
     4861 gctcgctcgc ggaatcggcg gtgcgcgagc acttcggcgt gacggtcgtc ggcatcaagc
     4921 gcgcacacga ggattttcag cacggcaagc cgggcagcgt cgtgttgccg ggtgacctgc
     4981 tgatcgtttc cggaccgacg aagaaggtcg aggcgtttgc ggccagcggt cggcgccgat
     5041 agctggccag cgtcgaggtg gcggctgcgc tgcgaccgtg ccggcctgtg caatgttctc
     5101 attcccggaa aggccgcgga agatgctcaa ctggatcctg aatcaattca agcgacagtc
     5161 ggccgaggat ctcgagcggg cgcgggaaat ggtaaaggcc gcggaaaggg ggcactcgca
     5221 cccggacctc gggaaggcac gcgatgttgc gcgcacgctc ggaatcgaag tggatgtcca
     5281 tgcgagcgcc gagcagctca tccaggcaat ccgccgttac ctcgcgcgcc acggcgagat
     5341 gtgaacccga ccgcgcaaga cgccgaacga gcgctcgagc cgggggacgc cgaccggcag
     5401 cacggcagct tttgaaggat cctcatcggc tgggaatctg tcgtgggaac atcggggtga
     5461 tgaatgtctc ttccgcatcc ggtcgagacc gccaccgtcg gtttgccgcg ccccaacgcg
     5521 ggttcggg
//
LOCUS       DAOQNN010000210         5518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8675_length_5518_cov_9.282262, whole genome shotgun sequence.
ACCESSION   DAOQNN010000210 DAOQNN010000000
VERSION     DAOQNN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8675_length_5518_cov_9.282262"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1052)
                     /locus_tag="PKV97_11085"
     CDS             complement(252..1052)
                     /locus_tag="PKV97_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNB06464.1"
                     /translation="MQPKKTESAPSAEGKTSIQVIERMMKLLDVLAEHADPVQLKQLA
                     IETGLHPSTAHRILGAMTHSGFVERSEAGAYRLGIRLLELGSLVKSRISLRETAMPYM
                     LKLHAATGESVNLGVRAGDEIVYVERTSSGRASVRVVHIVGARAPLHTTATGKLFLVE
                     DGLERIRDYARRTALPASTPASITALPALEKELDRVRRHGVAFDLDEVEAGVRCIAAG
                     IRDDSGELIAGLSLSTPSERFNPDWAPLVRETADEISRALGHLPPKAG"
     gene            1276..>2444
                     /locus_tag="PKV97_11090"
     CDS             1276..>2444
                     /locus_tag="PKV97_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNB06465.1"
                     /translation="MSLALIVLLPFIGALFPALMIRAGRSACATTAFVFPLLALVLLL
                     AQAPAVFRGEVLVAAWDWVPALGLQAGFFLDGLGMFFAGLILGIGLLVIVYARFYLAK
                     NDPMGVFYSYLLLFQGAMVGVVLSDNILLLLVFWELTSLSSFLLIGYWKHLPEGRQGA
                     RMALTVTGAGGLAMIAGMLILGDIAGSWNLSDILRNKEAIQASPLYVPALVLILAGCF
                     TKSAQFPFHFWLPHAMAAPTPVSAYLHSATMVKAGLFLMARMWPVLAGTTEWFYLVAT
                     TGLVTMLVAAGIALFKDDLKGLLAYSTVSHLGLVTMLLGFGTPLAAVAAVFHILNHAT
                     FKAALFMNAGIVDHEAGTRDIRRLGGLLQLMPITATMAMVAGASMAGLPPLNGFLS"
     assembly_gap    2445..2544
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2545..3954
                     /locus_tag="PKV97_11095"
     CDS             <2545..3954
                     /locus_tag="PKV97_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HNB06466.1"
                     /translation="VTGGAVMDYHLALWHGFTPALGLSAIAVALGLGALVAYRRVRAL
                     WQAGPHPEAKAMFDAFIEGAAALARGITHGLHNGSLQRYLAFALTAFTLAGFAAFLAA
                     PHAAGTRALLPAHPAAIAAWVLLVGGCALTVMLFRRRVLSVLVVGTVGLIVCLAFIYL
                     SAPDLALTQISVEVATVILILLALYFLPKEGPQSSSVRADPLRHLRDGVLAIVAGIGM
                     AGASWAMLTRDGSSLSSYYLDNSVSGGGGTNVVNVILVDFRGFDTFGEITVLGIAALA
                     IYALLDGALFGRTGRRLGAWSPDRPQSADRHPMIMVVATRVMLPLAMLVGAYIFLRGH
                     NQPGGGFIAALVVSIALIMQYMASGFGWAAHRVKVNYHAMIGLGVLVAAATGIGAMVL
                     DQPFLTSTFGHFHLPLVGEFELASAMAFDTGVFLTVVGAVMLALANLSRMGRWTSPYT
                     INTGAMDVDPRAASGKEQG"
     gene            3954..4370
                     /locus_tag="PKV97_11100"
     CDS             3954..4370
                     /locus_tag="PKV97_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HNB06467.1"
                     /translation="MEFLVASAIGVMTAAGIYMVLRARTFPVVIGLSLLTYATNLFLF
                     ATGRLAIGQPPVISAAATGYIDPLPQALVLTAIVISFGMTAVVVVMALRAYLETGNDH
                     VDLPGDASPEAAEDDWKLRARKPAPATARSGRGERA"
     gene            4367..>5518
                     /locus_tag="PKV97_11105"
     CDS             4367..>5518
                     /locus_tag="PKV97_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HNB06468.1"
                     /translation="MNHLLILPILLPAVVGALLVLAARHDGVLARTFAVASTVLLLAI
                     AVALLVLASDGQVRSYALGAWPAPFGIVLALDRLSALMLVLNAVLGLGVLLYAIGGWD
                     QRGKHFHPLFQFELMGINGAFLTGDFFNLFVFFEILLIASYGLMVHGGGGLRVKAGVQ
                     YVVTNLVGSSVFLIAVGMIYNVTGTLNMADLARIVPQVPAADQALLQTGAVLLLLVFA
                     VKAALVPLHFWLPGTYANAPGPVAALFAIMTKVGAYSILRLYVLAFGADAGAAAWLAA
                     PWLLPAGLLTLVLGMTGVLAARSLGQMASFAVIGSMGMLLATVAVFTPQATSAALYYL
                     IHSTLAAGALFLVADLVAERRGAQRDRFAAAPRIAQGGLVGGLFFLAAIA"
BASE COUNT          626 a         2023 c         1817 g          952 t
ORIGIN      
        1 ccgggtgtcg gtgtcttgcc gtgggagcga cgcaagtcgc gattgcagcc tttcctcctc
       61 gaccacgcct gtcgccgccg cggcgcagga tcgcgacttg cgtcgctccc acatcccacc
      121 ccgggcgccg tggtttttcc acctcccacc ccgggcgtcg tggttttccc gcacccggcc
      181 tcggggcatc ggtgtccgcg tgcagacctt gcggctttcg ccgctgcgag gggggctgcc
      241 cgcaagcggg cttatcccgc ctttggcgga aggtggccga gggcgcggga gatctcgtcg
      301 gcggtctcgc gcaccagtgg ggcccagtcg gggttgaagc gttcggaagg ggtcgatagc
      361 gacaggccgg cgatcagctc gccgctgtcg tcgcggatgc ccgcggcgat gcagcgcacg
      421 ccggcctcga cctcgtcgag gtcgaaggcc acgccgtggc ggcgcacgcg gtcgagctcc
      481 ttctccagcg ccggcagcgc ggtgatcgag gccggggtgg aggcgggcag ggcggtgcgg
      541 cgggcgtagt cgcggatgcg ctcgaggccg tcctcgacca ggaagagctt gccggtggcg
      601 gtggtgtgca gcggcgcgcg cgcgcccacg atgtgcacca cgcgcaccga ggcgcgcccg
      661 ctcgaggtgc gctcgacata cacgatctcg tcgccggcgc gcacgcccag gttcacgctc
      721 tcgccggtgg cggcgtgcag cttgagcatg tagggcatgg cggtctcgcg cagcgagatg
      781 cgcgacttca ccaggctgcc gagctcgagc aggcggatcc cgagccggta ggcgccggcc
      841 tcggagcgct cgacaaagcc gctgtgcgtc atcgcgccga ggatgcggtg cgcggtcgac
      901 ggatgcagcc cggtctcgat cgcgagctgc ttgagctgga ccggatcggc atgttcggcc
      961 agcacgtcga gcagcttcat catgcgttcg atgacctgga tcgaggtctt gccttcggca
     1021 ctgggggcag attcggtctt cttcggctgc acggaggttc ggggaaggat cggaagcttg
     1081 ccggggccgg caaggagatc tggcgaatgg tacccgtgcg atccgatttc gcctagtgaa
     1141 ataaaccccg atttggcggg ggtcgcatcc ccgatatact ccgcccggaa ttcgtcctca
     1201 tgcggggtgt gggtggcccc cgccacccgc gattcacgtc gcctttccca tcgaccacgc
     1261 ccgaggagcc ccccgatgag cctcgccctg atcgtgctgc tgcccttcat cggcgcgctc
     1321 tttcccgcgc tgatgatccg cgccggccgc agcgcctgcg cgaccaccgc cttcgtcttt
     1381 ccgctcctcg ccctcgtgct gctgctcgcg caggcgccgg cggtgttccg cggcgaggtc
     1441 ctcgtcgccg cctgggactg ggtgcccgcg ctcggcctgc aggcgggctt cttcctcgac
     1501 gggctgggca tgttcttcgc cgggctgatc ctgggcatcg gcctgctggt gatcgtctac
     1561 gcgcgcttct acctcgccaa gaacgacccg atgggcgtgt tctacagcta cctgctgctc
     1621 ttccagggcg cgatggtggg cgtcgtcctg tccgacaaca tcctgctgct gctggtgttc
     1681 tgggagctca ccagcctgtc gtccttcctg ctcatcggct actggaagca cctgcccgaa
     1741 ggccgccagg gtgcgcgcat ggcgctcacc gtcaccggcg ccggcgggct ggcgatgatc
     1801 gccggcatgc tgatcctggg cgacatcgcc ggctcctgga acctcagcga catcctgcgc
     1861 aacaaggaag cgatccaggc ctcgccgctg tacgtcccgg cgctggtgct gatcctcgcc
     1921 ggctgcttca ccaagagcgc gcagttcccc ttccacttct ggctgccgca cgcgatggcg
     1981 gcgcccaccc cggtgtcggc ctacctgcac tcggcgacca tggtgaaggc gggcctcttc
     2041 ctgatggcgc gcatgtggcc ggtgctcgcc gggacgaccg agtggttcta cctcgtcgcc
     2101 accaccggcc tggtgaccat gctggtggcg gccggcatcg cgctgttcaa ggacgacctc
     2161 aagggcctgc tggcctactc caccgtcagc cacctcggcc tggtgaccat gctgctcggc
     2221 ttcggcacgc cgctcgcggc ggtggccgcc gtgttccaca tcctcaacca cgccaccttc
     2281 aaggccgcgc tgttcatgaa cgccggcatc gtcgaccacg aggccggcac gcgcgacatc
     2341 cgccgcctcg gcggcctgct ccagctgatg ccgatcaccg cgaccatggc gatggtggcg
     2401 ggggcctcga tggccggcct gccgccgctc aacggcttcc tctcnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnngtcacc ggcggggcgg tgatggacta ccacctcgcg
     2581 ctctggcacg gcttcacccc ggcgctcggc ctgtcggcga tcgcggtcgc gctcggcctc
     2641 ggcgcgctcg tcgcctaccg gcgcgtgcgc gcgctctggc aggcggggcc gcaccccgag
     2701 gccaaggcga tgttcgacgc cttcatcgag ggcgcggccg cgctcgcccg cggcatcacc
     2761 cacggcctgc acaacggctc gctgcagcgc tatctcgcct tcgcgctcac cgccttcacg
     2821 ctcgccggct tcgccgcctt cctcgccgcg cctcacgccg cgggcacgcg cgcgctgctg
     2881 ccggcgcacc cggccgcgat cgccgcctgg gtgctgctgg tgggcggctg tgcgctcacc
     2941 gtgatgctgt tccgccgccg cgtgctgtcg gtgctggtgg tgggcacggt gggcctgatc
     3001 gtgtgcctgg ccttcatcta cctgtccgcg cccgacctcg cgctgacgca gatctcggtc
     3061 gaggtcgcca ccgtgatcct gatcctgctc gcgctctact tcctgcccaa ggaaggtccg
     3121 cagagctcct cggtgcgcgc cgatccgctg cgccacctgc gcgacggcgt gctcgccatc
     3181 gtggccggca tcggcatggc cggggcgagc tgggcgatgc tcacccgcga cggcagctcg
     3241 ctgtcgagct actacctcga caactcggtc tcgggcggcg gcggcaccaa cgtggtcaac
     3301 gtgatcctgg tcgacttccg cggcttcgac accttcggcg agatcaccgt gctcggcatc
     3361 gccgcgctgg cgatctacgc cctgctcgac ggcgcgctct tcggccgcac cggccgtcgt
     3421 ctgggcgcgt ggtcccccga ccgcccgcag tcggccgacc gccacccgat gatcatggtc
     3481 gtggccaccc gggtcatgct gccgctggcg atgctggtcg gcgcctacat cttcctgcgc
     3541 ggccacaacc agccgggcgg cggcttcatc gccgcgctcg tggtgtcgat cgcgctgatc
     3601 atgcagtaca tggcgagcgg cttcggctgg gcggcgcatc gggtcaaggt caactaccac
     3661 gccatgatcg gcctcggcgt gctggtggcc gccgccaccg gcatcggcgc gatggtgctc
     3721 gaccagccct tcctcacctc caccttcggc cacttccacc tgccgctggt gggcgagttc
     3781 gagctcgcca gcgcgatggc cttcgacacc ggggtcttcc tcaccgtggt cggcgcggtc
     3841 atgctggcgc tcgccaacct ctcgcgcatg gggcgctgga ccagccccta caccatcaac
     3901 accggcgcga tggacgtgga tccgcgcgcc gcctcgggca aggagcaggg ctgatggaat
     3961 tcctcgtcgc cagcgccatc ggcgtgatga ccgcggccgg catctacatg gtgctgcgcg
     4021 cgcgcacctt cccggtggtg atcggcctgt cgctgctcac ctacgccacc aacctgttcc
     4081 tgttcgccac cggccgcctg gcgatcggcc agccgccggt gatctccgcc gccgccacgg
     4141 gctacatcga cccgctgccg caggcgctcg tgctcaccgc gatcgtgatc tccttcggca
     4201 tgaccgcggt ggtggtggtg atggcgctgc gcgcctacct cgagaccggc aacgaccacg
     4261 tcgacctgcc cggcgacgcc tcgcccgagg ccgccgagga cgactggaag ctgcgcgccc
     4321 gcaagccggc gccggcgacg gcgcgcagcg gtcgcgggga gcgcgcatga accacctgct
     4381 gatcctcccc atcctgctgc cggccgtggt cggcgcgctg ctggtgctgg ccgcgcgcca
     4441 cgacggcgtg ctcgcgcgca ccttcgcggt ggcctccacc gtgctgctgc tcgccatcgc
     4501 cgtcgcgctg ctggtgctcg ccagcgacgg ccaggtgcgc agctacgcgc tcggcgcctg
     4561 gcccgcgccc ttcggcatcg tgctcgcgct cgaccgcctt tccgcgctga tgctggtgct
     4621 caatgccgtc ctcggcctcg gcgtgctgct gtacgcgatc ggcggctggg accagcgcgg
     4681 caagcacttc cacccgctct tccagttcga gctgatgggc atcaacggcg ccttcctcac
     4741 cggcgacttc ttcaacctct tcgtgttctt cgagatcctg ctgatcgcct cctacggtct
     4801 catggtgcat ggcggcggcg ggctgcgggt gaaggccggc gtgcagtacg tggtcaccaa
     4861 cctggtcggc tcctcggtgt tcctgatcgc ggtcggcatg atctacaacg tcaccggcac
     4921 cctcaacatg gccgacctcg cgcgcatcgt cccgcaggtg ccggcggccg accaggcgct
     4981 gctgcagacc ggcgcggtgc tgctgctgct ggtgttcgcg gtcaaggccg cgctcgtgcc
     5041 gctgcacttc tggcttcccg gcacctacgc caacgcgccc ggtccggtgg cggcgctgtt
     5101 cgcgatcatg accaaggtgg gcgcctattc catcctgcgc ctgtacgtgc tcgccttcgg
     5161 cgccgacgcc ggggccgcgg cctggctcgc cgcgccctgg ctgctgccgg ccgggctgct
     5221 caccctggtg ctgggcatga ccggcgtgct cgccgcgcgc agcctgggcc agatggccag
     5281 cttcgcggtg atcggctcga tgggcatgct gctcgccacc gtggcggtgt tcaccccgca
     5341 ggcgaccagc gccgcgctgt actacctcat ccactccacg ctcgccgccg gcgcgctctt
     5401 cctcgtcgcc gacctcgttg ccgagcgccg cggcgcgcag cgcgaccgct tcgccgccgc
     5461 gccgcgcatc gcccagggcg ggctcgtggg cggcctgttc ttcctcgccg ccatcgcc
//
LOCUS       DAOQNN010000211         5512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8689_length_5512_cov_8.829760, whole genome shotgun sequence.
ACCESSION   DAOQNN010000211 DAOQNN010000000
VERSION     DAOQNN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8689_length_5512_cov_8.829760"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKV97_11110"
     CDS             <1..374
                     /locus_tag="PKV97_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06469.1"
                     /translation="EGLALAALHAGRLLAVVCAVGILLERLPLARLVGGLYALSRPLR
                     LVGVAPERLALRLLLVLRYVEASPRGQGPADWRHWLADESPSDAAAEAAPVVLARERL
                     GLGEGLLAGALFGTLLWWSLA"
     gene            371..1168
                     /gene="truA"
                     /locus_tag="PKV97_11115"
     CDS             371..1168
                     /gene="truA"
                     /locus_tag="PKV97_11115"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNB06470.1"
                     /translation="MRRIVLGLEYAGDAFCGWQSQAHGRTVQDVLERALAQIAGEPVR
                     THCAGRTDTGVHASAQVAHFDTRAVRPASAWVRGVNALLPPAVVVRWSTEVGDDFHAR
                     FLACERQYRYILHNAPTRPAMLAGRVGWYHPALDVERMAAAAQCLVGTHDFSSFRAAG
                     CQAKTPVRVMHEARVVRQGEYLLFDFRANGFLHHMIRNLVGALVYVGKGRYPVAWMRE
                     LLEARDRARAAPTFSPDGLYLCGVAYPPRWSLPGDGRIIALPQLPLV"
     gene            1169..1792
                     /locus_tag="PKV97_11120"
     CDS             1169..1792
                     /locus_tag="PKV97_11120"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNB06471.1"
                     /translation="MFRTRIKICGLTRPQDVRAAVDSGADAIGFVFYPPSPRAVSIEQ
                     AAELVALLPPFVVSVGLFVNPAPGEVEAVLARVPLQLLQFHGDESESDCARHGRAWIK
                     AARMRPGVDLLEFAASYPRASGILVDAFVDGYGGGGRTFDWTLIPAGFKRPLVLSGGL
                     DADNVGDAVRRIRPWAVDVSSGVESAKGIKDADMIAAFIAGVRHADG"
     gene            1779..2990
                     /gene="trpB"
                     /locus_tag="PKV97_11125"
     CDS             1779..2990
                     /gene="trpB"
                     /locus_tag="PKV97_11125"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNB06472.1"
                     /translation="MQMADTPYQFPDASGHFGPYGGIFVAETLMPALAELREAYAACQ
                     NDPAFVAEFEYELKHYVGRPSPIYHAKRWSELLGGAQIYLKREDLNHTGAHKVNNCIG
                     QAMVARRMGKPRVIAETGAGQHGVATATVAARYGMECVVYMGSEDVKRQAANVYRMKL
                     LGATVVPVESGSKTLKDALNEAMRDWVTNVADTFYIIGTVAGPHPYPMMVRDFQSVIG
                     KECLVQMPDMVGRQPDYVIACVGGGSNAMGIFHPYIGVPGVRLVGVEAAGDGLDTGRH
                     SASLTAGRPGVLHGNRTYLLQDEDGQIIETHSVSAGLDYPGVGPEHAWLKDTARAEYV
                     GITDAEALEAFHKLCRLEGIIPALESSHALAYAAKLAPSLPKDKILLVNLSGRGDKDM
                     HTVAERSGIQF"
     gene            3046..3867
                     /gene="trpA"
                     /locus_tag="PKV97_11130"
     CDS             3046..3867
                     /gene="trpA"
                     /locus_tag="PKV97_11130"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNB06473.1"
                     /translation="MSRIQTVFQRLQADGRKALIPFVTAGFPAPALTLPLMNALVEGG
                     ADILELGVPFSDPMADGPTIQRASERALAQGMSLRKVLETVRAFRAANADTPVVLMGY
                     ANPIEAMGQERFVAAAHEAGVDGVLVVDYPPEECVSFAATAKAGGLDPIFLLAPTSGE
                     QRFADVARAGSGYIYYVSLKGVTGSAKLDVDEVARRIPEIRAAVGMPVGVGFGIRDAE
                     SAQRIGAVADAVVIGSRIIEEIENNPGDEVARVRDFMRPIRAALDALATGRGVQA"
     gene            3864..4739
                     /gene="accD"
                     /locus_tag="PKV97_11135"
     CDS             3864..4739
                     /gene="accD"
                     /locus_tag="PKV97_11135"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNB06474.1"
                     /translation="MSWFEKLIPPRIKRGDSSRSKSIPEGLWSKCEGCESVLYRSDLD
                     SNLGVCPKCGHHHRLRARRRLDQLLDPEGRVEIGAEVTPVDALKFKDSRKYPDRLSAA
                     AADTGEADAMVVMRGAIKTVPVVVACFEFEFLGGSMGSVVGERFVRGARAAFEERLPF
                     ICITASGGARMQEGLLSLMQMAKTTASITRLAERRLPFITLLTDPTMGGVSASFAFMG
                     DVVVAEPNALIGFAGPRVIEQTVREKLPEGFQRAEFLLEKGAIDLIVDRRELRDRLAV
                     LLAMLNRQPADVVVG"
     gene            4739..>5512
                     /locus_tag="PKV97_11140"
     CDS             4739..>5512
                     /locus_tag="PKV97_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNB06475.1"
                     /translation="MAEQAASPASLDGWLALLEQRHGQRIELGLERVAAVRARMQAES
                     DAVVITVGGTNGKGSCCAMLEGILLASGYRVGCYTSPHLLRYNERVRIDGRDADDAAL
                     VAGFAAVEAARGDTALTYFEHGTLAAWQVFAAAGLDVIILEVGLGGRLDAVNVFEPDC
                     ALVTSVAMDHMDYLGDTREAIGFEKAGIFRAGRPAVCGDPQPPATLVEHAAAIGAQLW
                     ISGRDFGFGGDRQQWGYWRYAQPAREGALVRRGGLAYPAL"
BASE COUNT          758 a         1969 c         1927 g          858 t
ORIGIN      
        1 gcgaggggct tgcgctcgcg gcgctgcatg ccgggcggct gctcgccgtg gtgtgcgcgg
       61 tcggcatcct gctcgagcgc ctgccgctcg cgcgcctggt cggtggcctg tacgcgctgt
      121 cgcgcccgct gcgcctggtc ggcgtggcgc ccgaacgcct cgccctgcgc ctcttgctgg
      181 tgctgcgcta cgtcgaggcc tcgccgcgcg ggcaggggcc ggccgactgg cgacactggc
      241 tggcagacga gtctccgtcc gacgcggccg ccgaagcggc gccggtggtg ctggcgcgcg
      301 agcgcctcgg actgggcgag gggctgctgg cgggtgcgct cttcggcacg ctgctgtggt
      361 ggagcctggc atgaggcgca tcgtgctcgg cctcgaatac gccggcgacg ccttctgcgg
      421 ctggcagagc caggcccatg gccgcaccgt gcaggacgtg ctcgagcgcg cgctcgcgca
      481 gatcgcgggc gagccggtgc gcacccactg cgccggacgc accgacaccg gcgtgcatgc
      541 gagcgcgcag gtggcgcatt tcgacacccg cgccgtgcgc ccggcctcgg cctgggtgcg
      601 cggtgtcaac gcgctgctgc cgccggccgt ggtggtgcgc tggtcgaccg aggtgggcga
      661 cgacttccac gcccgcttcc tggcctgcga gcggcagtac cgctacatcc tgcacaatgc
      721 gccgacgcgg ccggcgatgc tggccgggcg ggtgggctgg taccacccgg cgctcgacgt
      781 cgagcgcatg gccgccgccg cgcagtgcct ggtcggcacc cacgacttct caagttttcg
      841 cgctgccggc tgtcaggcga agacgccggt gcgggtgatg cacgaggccc gcgtggtccg
      901 ccagggcgag tacctgctgt tcgatttccg cgccaacggc ttcctccacc acatgatccg
      961 caacctggtc ggcgcgctgg tgtacgtcgg caaggggcgt tatccggtgg cgtggatgcg
     1021 cgagctgctc gaggcccgcg accgcgcgcg cgcggcgccg accttctccc cggacgggct
     1081 ctacctgtgc ggcgtggcct acccgccgcg ctggtcgctg ccgggtgatg ggcgtatcat
     1141 cgcgctgccc cagctacccc tcgtctgaat gttccgtacc cgaatcaaga tctgcggcct
     1201 gacccggccg caggacgtgc gcgcggctgt cgatagcggt gccgacgcga tcggcttcgt
     1261 gttctacccg cccagcccgc gtgcggtctc catagagcag gctgccgagc tcgtcgcgct
     1321 gctgcccccc ttcgtcgtct cggtcggcct cttcgtcaat cccgctcccg gcgaggtcga
     1381 ggcggtactc gcgcgcgttc cgctgcaact tctgcagttc cacggtgacg aaagcgagtc
     1441 cgactgtgcc cgtcacgggc gcgcgtggat caaggcggcg cgaatgcggc ccggggtcga
     1501 tctgctagaa ttcgcggctt cgtacccgcg cgcgagcggc atcctggtcg acgccttcgt
     1561 cgacggctat ggcggcgggg gcaggacctt cgactggacg ctgatccccg caggcttcaa
     1621 gcgtccgctc gtgctttcgg gcggactcga cgcggacaac gtgggcgacg ccgtgcgccg
     1681 catccgcccg tgggcggtgg atgtgtcgag cggggtcgag agtgccaagg gaatcaagga
     1741 cgcggacatg atcgccgcgt tcatcgccgg agttcgacat gcagatggct gacacgcctt
     1801 accagtttcc cgacgcgagc gggcatttcg ggccctacgg cggcatcttc gtcgccgaaa
     1861 ccctgatgcc ggcgctcgcc gagctgcgcg aggcctacgc ggcctgccag aacgatccgg
     1921 cgttcgtcgc cgagttcgag tacgagctaa agcactacgt cggccgcccg agcccgatct
     1981 accatgccaa gcgctggtcg gagctccttg gcggcgcgca gatctacctc aagcgcgagg
     2041 atctcaacca caccggcgcg cacaaggtga acaactgcat cggccaggcc atggtcgccc
     2101 gccgcatggg caagccgcgc gtgatcgccg agaccggtgc cggccagcac ggtgtggcca
     2161 ccgccaccgt ggccgcacgc tacggcatgg agtgcgtggt ctacatgggt tcggaagacg
     2221 tcaagcgcca ggccgccaac gtctaccgca tgaagctgct cggcgccacc gtggtgccgg
     2281 tcgagtcggg ctcgaagacg ctcaaggatg cgctcaacga agccatgcgc gactgggtga
     2341 ccaacgtcgc cgataccttc tacatcatcg gcaccgtcgc cggcccgcac ccgtatccga
     2401 tgatggtgcg cgacttccag tcggtgatcg gcaaggaatg cctggtgcag atgcccgaca
     2461 tggtgggccg ccagcccgac tacgtgatcg cctgcgtggg cggcggctcc aacgcgatgg
     2521 gcatcttcca tccctacatc ggcgtgccgg gcgtcaggct tgtcggcgtc gaagccgccg
     2581 gcgacggcct ggacacgggg cgccactcgg cctcgctgac cgccggccgc cccggcgtgc
     2641 tgcacggcaa ccgcacctac ctgctgcagg acgaggacgg gcagatcatc gagacgcact
     2701 cggtctccgc cggactcgac tacccgggcg tcggtcccga gcacgcctgg ctgaaggaca
     2761 ccgcacgcgc cgagtatgtc ggcatcaccg acgccgaggc gctcgaggcc ttccacaagc
     2821 tgtgccggct ggagggcatc attccggcgc tcgagtcctc gcacgcgctc gcctacgccg
     2881 ccaagctggc gccctccctg cccaaggaca agatcctgct ggtcaacctc tccgggcgcg
     2941 gcgacaagga catgcacacc gtcgccgagc gctccggcat ccagttctga acctgaatcc
     3001 gaacctgaag cgggccgctg atgcggcccg cagtgtgatc gagacatgtc gagaatccag
     3061 accgttttcc agcgcctcca ggccgatggc cgcaaggcgc tgattccctt cgtcaccgcc
     3121 ggcttcccgg cgcccgcgct caccctgccg ctgatgaacg cgctggtcga gggcggcgcc
     3181 gacatcctcg agctcggcgt gcccttctcc gacccgatgg ccgacggccc caccatccag
     3241 cgcgcctccg agcgcgcgct cgcgcagggc atgagcctgc gcaaggtgct cgagaccgtg
     3301 cgcgccttcc gcgccgccaa tgccgatacc ccggtggtgc tgatgggcta cgccaacccg
     3361 atcgaggcca tgggccagga gcgcttcgtc gctgccgcgc atgaagccgg agtcgatggc
     3421 gtgctggtgg tcgattaccc gccagaggag tgcgtgagct tcgccgccac cgccaaggcc
     3481 ggcggcctcg atcccatctt cctgctcgcg cccacctcgg gcgagcagcg cttcgccgat
     3541 gtcgcgcgcg ccggcagcgg ctatatctac tacgtctcgc tcaagggcgt gaccggcagc
     3601 gccaagctcg acgtcgacga ggtcgcccgc cgcattcccg agatccgcgc cgcggtcggc
     3661 atgccggtgg gcgtgggctt cggcatccgc gacgccgaga gcgcgcagcg catcggcgcg
     3721 gtggccgacg cggtcgtgat cggcagccgg atcatcgagg agatcgagaa caacccgggt
     3781 gacgaggtgg cgcgtgtgcg cgacttcatg cgcccgatcc gcgccgcgct ggacgcgctg
     3841 gcgacgggca ggggggtaca ggcatgagct ggttcgagaa actgatcccg ccgcgcatca
     3901 agcgcggcga ctcgtcgcgc agcaagtcga tcccggaagg gctgtggagc aagtgcgagg
     3961 gttgcgagtc ggtgctctac cgctccgacc tcgacagcaa cctcggcgtg tgcccgaagt
     4021 gcggccatca ccatcgcctg cgcgctcgcc gtcgcctcga ccagctgctc gaccccgagg
     4081 gccgcgtcga gatcggcgcc gaggtcacgc cggtcgatgc gctcaagttc aaggactcgc
     4141 gcaagtatcc cgatcgcctg tccgcggccg ctgccgacac cggcgaggcc gacgcgatgg
     4201 tggtgatgcg cggcgcgatc aagaccgtcc cggtggtggt cgcctgcttc gagttcgaat
     4261 tcctcggcgg ctcgatgggt tcggtggtcg gcgagcgctt cgtgcgcggt gcgcgtgcgg
     4321 ccttcgagga gcgcctgccc ttcatctgca tcaccgcctc gggcggcgcg cgcatgcagg
     4381 aaggcctgct ctcgctgatg cagatggcca agaccaccgc ctcgatcacc cgcctggccg
     4441 agcgccgcct gcccttcatc accttgctga ccgaccccac catgggcggc gtgtcggcga
     4501 gctttgcatt catgggcgac gtggtcgtcg ccgagcccaa cgcgctgatc ggcttcgccg
     4561 gcccgcgcgt gatcgagcag accgtgcgcg agaagctgcc cgaaggcttc cagcgcgccg
     4621 agttcctgct cgagaagggt gcgatcgacc tgatcgtcga ccgtcgcgag ttgcgcgacc
     4681 gcctcgcggt gctgctcgcc atgctcaacc gccagcctgc ggacgtggtc gtcgggtgat
     4741 ggcggagcaa gccgcatcgc ccgccagcct ggacggctgg ctcgccctgc tggagcagcg
     4801 ccacggccag cgcatcgagc tcgggctcga gcgcgtcgcc gcggtgcgtg cgcgcatgca
     4861 ggccgagtcg gacgccgtgg tgatcaccgt cggcggcacc aacggcaagg gctcgtgctg
     4921 cgccatgctc gaaggcatcc tgctcgcctc gggctaccgc gtgggctgct acacctcgcc
     4981 ccacctgctg cgctacaacg agcgcgtgcg catcgacggc agggacgccg acgacgcggc
     5041 cctggtggcc ggattcgccg ccgtcgaggc cgcccgcggc gacaccgcgc tgacctattt
     5101 cgagcacggc accctggcgg cctggcaggt cttcgccgcg gcagggctgg acgtgatcat
     5161 cctcgaggtc ggcctcggcg gccggctcga cgcggtcaac gtgttcgagc ccgactgcgc
     5221 gctggtgacc agcgtggcca tggaccacat ggactacctc ggcgacacgc gcgaggcgat
     5281 cggcttcgag aaggcgggca ttttccgcgc cggccgtccg gccgtgtgcg gcgatccgca
     5341 gccgcccgcg accctggtcg agcacgccgc ggcgatcggc gcgcagctgt ggatcagcgg
     5401 gcgcgacttc ggctttggcg gcgaccgtca gcagtggggc tactggcgct acgcccagcc
     5461 tgcgcgcgag ggtgcgctgg tgcgccgtgg cgggctcgcc tatccggcgc tg
//
LOCUS       DAOQNN010000212         5477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8781_length_5477_cov_10.614165, whole genome shotgun sequence.
ACCESSION   DAOQNN010000212 DAOQNN010000000
VERSION     DAOQNN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8781_length_5477_cov_10.614165"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKV97_11145"
     CDS             complement(<1..369)
                     /locus_tag="PKV97_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNB06476.1"
                     /translation="MSLALVHARALDGIEAPPVTVEVHLGNGLPQFHLVGLADTEVRE
                     ARDRVRAAIQSAQFEFPQRRITVNLAPADLPKEGGRFDLAIAIGILAASGQVAERLLP
                     RLELCGELSLNGGLRPVRGVL"
     gene            complement(641..892)
                     /locus_tag="PKV97_11150"
     CDS             complement(641..892)
                     /locus_tag="PKV97_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HNB06477.1"
                     /translation="MSTPRILDEIGARLSELAANSPVRDIEKNAKAMLGSAFGKLDLV
                     TREEFEVQREVLAQARRKLAELEARVAELEAKLAGGDKA"
     assembly_gap    1098..1197
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1471..2175
                     /locus_tag="PKV97_11155"
     CDS             1471..2175
                     /locus_tag="PKV97_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HNB06478.1"
                     /translation="MHTKPLATALAILATIATAGTAHAGDGPFTANVGLVSDYAYRGW
                     SQTDERPALQGGFDYAHDSGLYAGVWGSNVSWLSDANTDVSNSLELDLYGGYKGTVGA
                     IGYDVGLLQYYYPGSYPKPYNSPNTLEGYVGLSWEFLSFKYSYAFTDLFGYDKSDGSQ
                     YYDLGAAVDVGGGFTLAAHVGYSDIKGQDDYTDWKLGVTKEFGGFNFGLHYVDTDVDN
                     ADLADERVILSIAKSF"
     gene            2205..2543
                     /gene="glnK"
                     /locus_tag="PKV97_11160"
     CDS             2205..2543
                     /gene="glnK"
                     /locus_tag="PKV97_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNB06479.1"
                     /translation="MKFIAAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEAAIPDDILDQAIEAIEKSAATGKIGDGKIFVFELEQAIRI
                     RTGETGPEAL"
     gene            2558..3880
                     /gene="amt"
                     /locus_tag="PKV97_11165"
     CDS             2558..3880
                     /gene="amt"
                     /locus_tag="PKV97_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNB06480.1"
                     /translation="MRKLLAHLPAAALLAGASGGAFAQEAAAAVAEVHKGDVAWIMTS
                     TLLVLMMALPGLALFYGGMVRAKNMLSVLMQVMTVFSLNALLFAIYGYSLTFTEASPF
                     IGSLDKVFLSGVTIETLADTFTETVKLPEFAFIAFQATFAGITGALIVGAFAERMKFS
                     AVLIFSVIWFTLCYLPICHMVWGPGGYLLEDGALDFAGGTVVHINAGIAGLVGAWMVG
                     KRIGYGREALAPHSLTLTMVGASLLWVGWFGFNAGSNLEATAGAALAFINTLVATAAA
                     VLAWSLGEALIKGKPSMLGAASGAVAGLVAITPACGSVGPMGAIVIGTLSGFICLWGV
                     NGLKHMLGADDALDVFGVHGVGGIVGAILTGVFAAPSLGGTGAEDFSIASQVWIQTWS
                     VLVTIVWSAVVAFVAYKVADLLVGLRVPEDEERQGLDTTAHGETAYRY"
     gene            4111..4584
                     /locus_tag="PKV97_11170"
     CDS             4111..4584
                     /locus_tag="PKV97_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06481.1"
                     /translation="MRHKIAPLRLLSLAALVTGCASITTGHNQSLSVETRLDGQPAPG
                     ASCKLENDKGAWFATSPGTVVVHRSYNDLNVRCEKDGHEPGVAAARSSTKGMAFGNIL
                     AGGIIGAAIDAGSGAAYDYPSIISVELGRKRSLPAEPPPDASTPRIAPQPLGNDT"
     gene            4704..>5477
                     /gene="csm6"
                     /locus_tag="PKV97_11175"
     CDS             4704..>5477
                     /gene="csm6"
                     /locus_tag="PKV97_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated ring nuclease Csm6"
                     /protein_id="HNB06482.1"
                     /translation="MNPNLPATYPRRILLAVTGLSPQIVTETLHALAIGNDTPFIPTE
                     IHLITTAEGADRARLALLSDRLGWFHRLRRDYALPEIAFDVDHIHILRAPSGALMHDI
                     RSPADNLVCADFITEKVRELSADPGAALHVSIAGGRKTMGFFLGYALSLFGRPQDRLS
                     HVLVSEPFENTWDFFYPTPYENIIETSGKKLVDARDARLSLAEIPFVSLRHGLPTALL
                     DGSARFEETVGAARAALGPAHLELDLRARSVRAAGREFRL"
BASE COUNT          887 a         1948 c         1703 g          839 t
ORIGIN      
        1 gagcacccca cgcaccgggc gcaatccgcc gttcagcgaa agctcgccgc acagctccag
       61 gcgcgggagc agccgctcgg ccacctggcc ggaggcggcg aggatgccga tcgcgatcgc
      121 gagatcgaag cgcccgccct ccttgggcag gtctgcaggc gccaggttga cggtgatgcg
      181 gcgctgggga aactcgaact gcgccgactg gatcgccgca cgcacgcggt cgcgcgcctc
      241 gcgcacctcg gtgtcggcga ggccgacgag atggaactgg ggcaggccgt tgcccaggtg
      301 gacctcgacc gtgaccgggg gcgcctcgat cccgtcgagc gcgcgcgcat gaacgagggc
      361 gagcgacatc tgcgactccg gacggaaagg aatcgtcagt ctagcggagc cgcggtcgat
      421 gacaaagaaa tatgcggaac gcgggaccgg gggcattcgg tcacgagacg catacggtgt
      481 ttcatgcaga agcgaacgca gcacgtagcg tgtcgcgacg cccatcgccg cccaccgtcc
      541 gttcgcggct ggcgccgctc ctacgaggtt tccgggaacc ttgccggttt ccgcaggagc
      601 gccgccagac gcgaatgcag cgtgtggaaa cgcagctgga tcaggccttg tcgccgccgg
      661 ccagcttcgc ttccagttcg gcgacgcgcg cctcgagctc ggcgagcttg cgccgcgcct
      721 gcgcgagcac ctcgcgctgc acctcgaatt cctcgcgggt gaccaggtcg agcttgccga
      781 aggccgagcc gagcatggcc ttggcgttct tctcgatgtc gcggacgggg ctgttggcgg
      841 cgagttcgga gaggcgggcg ccgatttcgt cgaggatgcg gggcgtgctc atgatggcgt
      901 ggattccggg ttgcagaggc gtcggagtct agcacgcacg cccctgcgga ctggagcgct
      961 gccggcccgc cgcacacgat cggcgcaagc gcggtgcgcg cgacattggc gcgatcacga
     1021 cttgcgtcgc tccggcggga gcccgggcga cgcaggggtg ggcgaaggtt ttcgtaggag
     1081 cgacgcaagt cgcgatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg
     1201 ggcgacgcag gggtgggcga aggttttcgt aggagcgacg caagtcgcga tctggcgcca
     1261 agaaccgtca accatcgctc gcacaacacg aatggcgcac cacattggtg cgcaacgccc
     1321 cgaaatcgcg cgcccatccc tgccgcagcg cagcaagatc cgcccgccga caagcggaaa
     1381 cctaggaatc accaggcttt ccgcaccctg gcacgccacg tgcttaggga gtctcgtcgc
     1441 gaactttcca aacctcgtca ggagatgacc atgcacacga aaccgctcgc caccgcgctc
     1501 gccatcctcg ccacgatcgc caccgccggc accgcccatg ccggcgacgg ccccttcacc
     1561 gccaacgtcg gcttggtgtc cgactacgcc taccgcggct ggagccagac cgacgagcgt
     1621 cccgcgctgc agggcggctt cgactacgcc cacgacagcg gcctgtacgc cggcgtgtgg
     1681 ggctcgaacg tatcgtggct gtcggacgcc aacacggacg tcagcaacag cctcgagctc
     1741 gacctctacg gcggttacaa ggggacggtc ggcgccatcg gctacgacgt cggcctgctg
     1801 cagtactact accccggcag ctaccccaag ccctacaaca gcccgaacac gctcgagggc
     1861 tatgtcggcc tgtcgtggga gttcctgtcc ttcaagtact cgtacgcctt caccgacctg
     1921 ttcggctacg acaagtcgga cggcagccag tactacgacc tcggcgccgc ggtcgatgtc
     1981 ggcggcggct tcaccctcgc cgcccacgtc ggttacagcg acatcaaggg ccaggacgac
     2041 tacaccgact ggaagctggg cgtcaccaag gagttcggcg gcttcaactt cggcctgcac
     2101 tacgtcgaca ccgacgtgga caacgccgac ctcgccgacg agcgcgtgat cctgtcgatc
     2161 gccaagagct tctgagcatc gcccatccgg aaccggagaa agacatgaaa ttcatcgctg
     2221 caatcatcaa gcccttcaag ctcgacgagg tccgcgaggc gctctcggcc atcggcgtgc
     2281 agggcatcac cgtgaccgag gtcaagggct tcggccgcca gaagggccac accgagctct
     2341 atcgcggcgc cgagtacgtc gtcgacttcc tgcccaaggt caaggtcgag gccgccatcc
     2401 ccgacgacat cctcgaccag gcgatcgaag cgatcgagaa gtccgccgcc accggcaaga
     2461 tcggcgacgg caagatcttc gtcttcgaac tcgagcaggc catccgcatc cgcaccggcg
     2521 agaccggccc ggaagcgctg taagggagca acgcaaaatg agaaaactcc tcgcacatct
     2581 gccggcggca gccctgctcg ccggcgcctc cggcggcgcc ttcgcccagg aggccgcggc
     2641 cgccgtggcc gaggtgcaca agggcgacgt ggcctggatc atgacctcca cgctgctcgt
     2701 gctgatgatg gccctgcccg gcctggcgct gttctacggc ggcatggtgc gcgccaagaa
     2761 catgctctcg gtgctcatgc aggtcatgac cgtgttctcg ctcaacgccc tgctgttcgc
     2821 gatctacggc tacagcctga ccttcaccga agccagcccc ttcatcggct cgctcgacaa
     2881 ggtcttcctg tccggggtga cgatcgagac gctcgccgac accttcaccg agaccgtgaa
     2941 gctgcccgag ttcgccttca tcgccttcca ggccaccttc gccggcatca ccggtgcgct
     3001 catcgtcggc gccttcgccg agcgcatgaa gttctccgcg gtgctgatct tctcggtgat
     3061 ctggttcacg ctgtgctacc tgccgatctg ccacatggtg tggggcccgg gcggctacct
     3121 gctcgaagac ggcgcgctcg acttcgccgg cggcaccgtg gtgcacatca acgccggcat
     3181 cgccggtctg gtgggcgcct ggatggtggg caagcgcatc ggctacggcc gtgaggcgct
     3241 cgcaccgcac agcctgaccc tgaccatggt cggcgcctcg ctgctgtggg tgggctggtt
     3301 cggcttcaac gccggatcca acctcgaagc caccgccggc gccgcgctcg ccttcatcaa
     3361 caccctggtc gccaccgccg ccgcggtgct cgcctggtcg ctcggcgagg cgctcatcaa
     3421 gggcaagccc tcgatgctgg gcgcggcctc gggtgcggtc gccggcctgg tggcgatcac
     3481 cccggcctgc ggctcggtcg gcccgatggg cgcgatcgtg atcggcaccc tgtccggctt
     3541 catctgcctg tggggcgtga atgggctcaa gcacatgctc ggtgcggacg acgcgctcga
     3601 cgtgttcggg gtgcatggcg tgggcggcat cgtcggcgcg atcctcaccg gcgtgttcgc
     3661 cgcgcccagc ctgggcggca ccggcgccga ggacttctcg atcgcctcgc aggtgtggat
     3721 ccagacctgg agcgtgctcg tgaccatcgt gtggtcggcc gtggtcgcct tcgtcgccta
     3781 caaggtcgcg gacctgctgg tcgggctgcg cgtgccggaa gacgaggagc gtcagggcct
     3841 cgacaccacg gcgcacggcg agaccgccta ccgctactga gccggcttgc ggaaccgtgc
     3901 tcctccggtt ccgatccact cgcggcgccc ttcggggcgc cgtttttctt tggcgcgccc
     3961 ccaatggaag cgatcgggcg caacgccctg ccggcattcg cgcagcgcaa gcctgccagc
     4021 ggcaccgaca ccgcaagctt gccgttcgac cctccgcacg atgacatcgg gagaatcccc
     4081 ggggtccaat ccaccccgag gcaacgcccc atgcgtcaca agatcgcccc tctccgcctg
     4141 ctttccctcg ccgcactcgt taccggctgt gcctcgatca cgaccggtca caaccagagt
     4201 ctcagcgtcg aaacgcgcct cgacggccag cccgccccgg gcgcgagctg caagctcgag
     4261 aacgacaagg gcgcctggtt cgccaccagc ccgggaacgg ttgtcgtgca ccgctcgtac
     4321 aacgacctga acgtgcgctg cgaaaaggac gggcatgaac ccggcgtcgc tgccgcccgc
     4381 tccagcacca agggcatggc cttcggcaac atcctggccg gcggcatcat cggcgccgcc
     4441 atcgatgccg gctcgggcgc ggcctatgac tatccgagca tcatcagcgt cgaactcggc
     4501 cgcaaacgct ccctgcccgc cgagcccccg cccgatgcca gcacgccacg catcgctcca
     4561 caaccgctcg gtaacgacac ctgacgcccg gcacgcgaca cgccaagccg tgaagccccg
     4621 cacggcaagc tcgccggcgg gcgacggagc ccggctcaac cgatatatgc ttctgcaatg
     4681 tcatgtggga ataccgtttt cgaatgaatc cgaatctacc tgcaacgtat ccaaggcgga
     4741 tcctgctcgc cgtgaccggg ctgtcgccgc agatcgtcac cgaaaccctg catgcgctcg
     4801 cgatcggcaa tgacacgccc ttcatcccga ccgaaattca cctgatcacc accgccgagg
     4861 gggccgaccg cgcccgcctc gcgctgctgt cggaccggtt gggctggttc caccgcctgc
     4921 gccgcgacta tgcgctgccc gagatcgcct tcgacgtcga tcacatccac atcctgcgcg
     4981 ccccctccgg ggcgctcatg cacgacatcc gcagccccgc ggacaacctg gtctgcgccg
     5041 acttcatcac cgaaaaggtc cgcgaactga gcgcagaccc cggcgccgcc ctgcacgtct
     5101 cgattgccgg cgggcgcaag accatgggct tcttcctcgg ctacgccctc tccctcttcg
     5161 gccgcccgca ggaccgcctc tcccacgtgc tcgtgtccga gccgttcgag aacacctggg
     5221 actttttcta ccccacgccc tacgagaaca tcatcgagac cagcggcaag aagctggtgg
     5281 acgcccgcga tgcccgcctg agcctggcgg agatcccatt cgtcagcctg cgccacggcc
     5341 ttcccaccgc gctgctcgac ggcagcgcgc gcttcgagga gacggtcggt gccgcacgtg
     5401 ccgcgctggg cccggcgcac ctggagctcg atctgcgcgc gcgctcggtc cgcgccgccg
     5461 gccgcgagtt ccgcctg
//
LOCUS       DAOQNN010000213         5473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8788_length_5473_cov_9.760244, whole genome shotgun sequence.
ACCESSION   DAOQNN010000213 DAOQNN010000000
VERSION     DAOQNN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8788_length_5473_cov_9.760244"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..488
                     /gene="recX"
                     /locus_tag="PKV97_11180"
     CDS             39..488
                     /gene="recX"
                     /locus_tag="PKV97_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HNB06483.1"
                     /translation="MGEPSLRERALRHLARRDHSRAELARKLAPHGEPAEIEAVLERM
                     RELGLQSDERYAEAFVRGKAARFGTARLRNELVRRGIDRDRIDEALADECVESEIERA
                     RAVLHGRFATPPADAREWARQARFLQTRGFAPELIRKLLKEPYDESA"
     gene            475..1197
                     /gene="lptB"
                     /locus_tag="PKV97_11185"
     CDS             475..1197
                     /gene="lptB"
                     /locus_tag="PKV97_11185"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNB06484.1"
                     /translation="MSLLKVAGLQKRYKARTVVHDVGFEVGSGEVVGLLGPNGAGKTT
                     CFYMIVGLVRADGGQIALDGTDLTHLPIHARARLGLSYLPQEMSVFRKLSVAENIRAV
                     LELQGLERRQIEERLEELLMELGIHHLRDNTAISLSGGERRRCEIARALATDPRLILL
                     DEPFAGVDPIAVIDIQKIIRFLKERGIGVLITDHNVRETLGICDRAYIISEGRVLASG
                     KPTEIIENERVRQVYLGEHFRL"
     gene            1220..2674
                     /locus_tag="PKV97_11190"
     CDS             1220..2674
                     /locus_tag="PKV97_11190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNB06485.1"
                     /translation="MKPSLQLKLSQHLTLTPQLQQSIKLLQLSTIELNQEIERVLLEN
                     PMLEREDGDGGVDYAPAPPPGNAPEREAAPEPAAEPAGESITHEGGEGSSEDEGIDWS
                     NVGQGASSRSEDDEDGDYQDIQAAGVSLREHLDQQIALSPLSDRDRALVRFLIEALDD
                     DGYLHQPLEDLLELLPQDAEVDLDELAIALRHVQSLEPAGIGARSPQECLALQLQALP
                     ECPIRPLALEIVTSHLELLAERNFARIRKLTGCDDEGLREAQALICSLDPHPGSRYST
                     AETRYVLPDVVVRKLRGQWTVSLNQEAMPRLRINRLYASLLQQNRAQGGGLGGQLQEA
                     RWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGDVAMRPLTLREIADQLELHESTVSRV
                     TTQKYMATPRGVFELKYFFGSHVATDTGGAASSTAIRALIRQLVDAEDSKKPLSDAKI
                     AEILGQQGIVVARRTIAKYRESLNIPPVSLRKSL"
     gene            2693..3016
                     /gene="raiA"
                     /locus_tag="PKV97_11195"
     CDS             2693..3016
                     /gene="raiA"
                     /locus_tag="PKV97_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNB06486.1"
                     /translation="MNLNITGRHVEVTPAIREYVTSKLDRVIRHFDNVTSVTVILSVE
                     KLKQKAEVTLHVRGKDLFVEADDADLYAAIDAMTDKLDRQVQKYKQKQSDHNHESLKH
                     QVPEA"
     gene            3153..3638
                     /gene="ptsN"
                     /locus_tag="PKV97_11200"
     CDS             3153..3638
                     /gene="ptsN"
                     /locus_tag="PKV97_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HNB06487.1"
                     /translation="MSLISQLLPLSNVVIDLDASSKKRVFEQAGLLFENNQGIARSTV
                     FDSLFTRERLGSTGLGQGIAIPHGRIKGLKDAAGAFLRLATPVQFDAPDGRPVDMLFV
                     LLVPEQANETHLQLLSELAQMFSDREFRETLHGATDAAQIHNLFVNWGADAANQPDPA
                     V"
     gene            3607..4554
                     /gene="hprK"
                     /locus_tag="PKV97_11205"
     CDS             3607..4554
                     /gene="hprK"
                     /locus_tag="PKV97_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNB06488.1"
                     /translation="MRQTSLTRLYEAHRESLSLVHVSGPIGARLSVAEERIWPADLVG
                     HLNLIHPTRLQVLGAAELAWAQRQANEKVAHHLNEILSAHPPAIIVADGCEIPNILHG
                     ICEAHDVALFTARHPSASVIDQLRLYLSRELAEKVSLHGVFMDVLGLGVMITGDSGAG
                     KSELGLELISRGHGLVADDVVELSRIAPTVLEGRCPEMLQDFIEVRGLGLINIRTIFG
                     ETACRRKMRLRLICHLDRRQPGPDDPCRLPLQQETQDILGVKVARVVLPVAAGRNLAV
                     LLEAAVRSTILKLRGIDSTQEFIDRQARALHAEEAYRAD"
     gene            4627..>5473
                     /gene="ilvA"
                     /locus_tag="PKV97_11210"
     CDS             4627..>5473
                     /gene="ilvA"
                     /locus_tag="PKV97_11210"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HNB06489.1"
                     /translation="MSSTPDYLERILNAQVYDVAVETPLDLATNLSVRTHNRIYFKRE
                     DMQPVFSFKIRGAYNKMANLSPQALKRGVICASAGNHAQGVALSAARLGVRAVIVMPS
                     TTPQIKIDAVKARGGEVVLAGESYSDAYTHALELEKSEKLTFVHPFDDPDVIAGQGTV
                     GLEILRAHPKPIHAVFCCVGGGGLIAGVAAYIKRLRPETKIIGVEAVDADAMTRSLAA
                     GKRIALEQVGIFADGAAVKEVGAETFRLCQQYVDEMIVVDNDAICAAIKDVFEDTRSI
                     LEPAGA"
BASE COUNT          968 a         1999 c         1715 g          791 t
ORIGIN      
        1 cccggcgccg gccgaggcct gaccccgtcg acgccgacat gggtgagccg agcctgcgcg
       61 aacgtgcgct ccgccatctc gcccggcgcg accactcgcg cgccgaactt gcgcgcaagc
      121 tcgcacccca tggcgagccg gccgagatcg aagcggtgct cgagcgcatg cgcgagctcg
      181 gcctgcagtc cgacgagcgc tacgccgagg ccttcgtgcg cggcaaggcg gcgcgcttcg
      241 gcaccgcacg attgcgcaac gagctcgtgc ggcgaggcat cgaccgtgac cggatcgacg
      301 aggcgctcgc cgacgagtgc gtagaatccg agatcgagcg ggcgcgcgcg gtgctgcacg
      361 gccgcttcgc caccccaccc gccgacgcac gcgaatgggc gcgccaggcg cgcttcctgc
      421 aaacccgggg cttcgccccc gagctgatcc gcaagctgct gaaagagccc tacgatgagt
      481 ctgcttaagg tcgccggcct gcaaaagcgc tacaaggcac gcaccgtggt gcatgacgtc
      541 ggcttcgagg tcggctcggg cgaggtcgtc ggcctgctcg gtcccaacgg cgccggcaag
      601 accacctgct tctacatgat tgtgggtctg gtgcgcgccg acggcggcca gatcgccctc
      661 gacggcaccg acctcaccca cctgccgatc cacgcccgcg cgcgcttggg cctgtcctac
      721 ctgccgcagg agatgagcgt gttccgcaag ctcagcgtgg ccgagaatat ccgcgccgtg
      781 ctcgaactcc aggggcttga acgccgccag atcgaggagc ggctcgagga actgctgatg
      841 gaactgggca tccatcacct gcgcgacaac accgcgatct cgctctcggg cggggagcgc
      901 cggcgctgcg agatcgcccg cgcgctcgcc accgacccgc gcctgatcct gctcgacgag
      961 cccttcgccg gggtcgatcc gatcgcggtc atcgacatcc agaagatcat ccgcttcctc
     1021 aaggagcgcg gcatcggcgt gctgatcacc gatcacaatg tgcgcgagac cctgggcatc
     1081 tgcgaccgcg cctacatcat cagcgaaggc cgcgtgctgg caagcggaaa acctaccgag
     1141 atcatcgaga acgagcgcgt gcgccaggtc tacctcggcg agcacttccg cctgtgagcc
     1201 cgccctcctg ccgaccgcga tgaaacccag cctccagctc aagctctccc agcacctcac
     1261 gctgacgccc cagctgcagc agtcgatcaa gctgctgcag ctgtctacga tcgagctgaa
     1321 ccaggagatc gagcgcgtgc tgctcgagaa tcccatgctc gagcgcgagg atggcgacgg
     1381 cggcgtcgac tacgcccccg cgccgccgcc gggcaacgcg cccgagcgcg aagccgcgcc
     1441 ggaacccgcc gccgaaccgg cgggcgagtc gatcacgcac gagggcggcg aaggcagcag
     1501 cgaggacgag ggcatcgact ggtccaacgt gggtcagggc gcgtccagcc gcagcgagga
     1561 cgacgaggac ggcgactacc aggacatcca ggccgccggc gtgtcgctgc gcgaacacct
     1621 cgaccagcag atcgccctct cgcccctgtc cgaccgcgac cgcgcgctgg tgcgcttcct
     1681 gatcgaggca ctcgacgacg acggctacct gcaccagccg ctggaagatc tcctcgagct
     1741 gttgccgcag gacgccgagg tcgatctcga cgagctcgcc atcgccctgc gccacgtgca
     1801 gagcctcgag ccggccggga tcggcgcgcg cagcccgcag gaatgtctgg ccctgcagct
     1861 ccaggccttg cccgagtgcc cgatacgccc tctcgctctc gagatcgtga ccagccacct
     1921 cgaactgctc gcggagcgca acttcgcccg tatccgcaag ctcaccggct gtgacgacga
     1981 gggtctgcgc gaagcgcagg ccctgatctg cagcctcgac ccccaccccg gctcgcgcta
     2041 ctccaccgcc gagacgcgct acgtgctgcc cgacgtcgtc gtgcgcaagc tgcgcggcca
     2101 atggaccgtg agcctgaacc aggaagcgat gccgcgtctg cgcatcaacc gcctgtacgc
     2161 gagcctgttg cagcagaacc gcgcacaggg cggcggcctc ggcgggcagc tgcaggaggc
     2221 gcgctggctg atcaagaacg tccagcagcg cttcgacacc atcctgcgcg tgtcccaggc
     2281 catcgttgac cagcaacggc agttcttcga tcatggggac gtggcgatgc ggccactcac
     2341 cctaagggag atcgcagacc agctcgagct gcacgaatcc accgtctcgc gcgtcaccac
     2401 gcagaagtac atggcgacgc cgcgcggagt cttcgagctc aagtattttt tcggcagtca
     2461 tgtcgcgacc gacaccggcg gtgcggcttc ctccacggcg attcgtgcgc tgattcgcca
     2521 gctggtggac gcggaggaca gcaagaagcc gctgtccgac gccaagatcg ccgaaattct
     2581 cggccaacag ggaatcgtcg tggcgaggcg cacgatcgcc aagtaccgcg agtccctcaa
     2641 catcccgccg gtcagcctgc gcaaatccct ctgacaacaa caggagtcat ccatgaatct
     2701 gaacatcacc gggcgccacg tcgaagtcac ccccgccatc cgcgaatacg tgacatccaa
     2761 gctcgatcgc gtcatccgcc atttcgacaa cgtcaccagc gtcaccgtca tcctgtcggt
     2821 cgaaaagctc aagcagaaag ccgaggtgac gctgcacgtg cgcggcaagg acctcttcgt
     2881 cgaagcggac gacgccgacc tctacgcggc gatcgacgcg atgaccgaca agctcgaccg
     2941 tcaggttcag aagtacaagc agaagcagtc cgaccataac cacgagtcgc tcaagcacca
     3001 ggtccccgag gcctgaggac cggcttgaag ctccccgtcc ggggtgtggt tataatgcgc
     3061 gcgttttcac gaagcgcggc cgcacacgcc ccggaccgga ttcccgcccc cctggcggca
     3121 gctgacggaa gagtgcgcga gcccacctgc gaatgagcct gatatcccaa ctcctgccgc
     3181 tttccaacgt ggtgatcgat ctcgacgcga gcagcaagaa gcgcgtcttt gaacaggccg
     3241 gcctgctgtt cgagaacaac cagggcatcg cccgcagcac cgtgttcgac agcctgttca
     3301 cccgcgaacg cctcggctcg accggactgg gtcagggcat cgccatcccg cacggccgca
     3361 tcaaggggct caaggacgcc gccggcgctt ttctgcgcct ggccaccccg gtgcagttcg
     3421 atgctcccga cggccgtccg gtggacatgc tgttcgtgct gctcgtgccc gaacaggcca
     3481 acgaaaccca tctgcagctg ctctccgaac tcgcgcagat gttcagcgac cgcgagttcc
     3541 gcgagaccct gcacggcgcg acggatgccg cacagatcca caacctgttc gtgaattggg
     3601 gtgccgatgc ggcaaaccag cctgacccgg ctgtatgagg cgcaccgcga aagcctgagc
     3661 ctcgtccacg tcagcgggcc gatcggtgcc cgtctgtcgg tcgccgaaga gcgcatctgg
     3721 ccggccgacc tcgtcggcca cctcaacctg atccacccca cccggctaca ggtgctcggc
     3781 gccgcagagc tcgcgtgggc acaacgccag gcgaacgaga aggtcgccca ccacctcaac
     3841 gagatcctct cggcgcaccc ccccgcgatc atcgtcgccg acggctgcga gatccccaac
     3901 atcctccacg gcatctgcga ggcgcacgac gtcgcgctgt tcaccgcgcg ccacccctcg
     3961 gcgagcgtga tcgaccagct ccgcctctac ctgtcgcgcg agctcgccga gaaggtctcg
     4021 ctgcacggcg tgttcatgga cgtgctcggg ctcggcgtga tgatcaccgg cgattccggc
     4081 gcgggcaaat ccgagctcgg cctcgagctg atctcgcgcg gccacggcct cgtcgccgac
     4141 gacgtggtcg agctgtcgcg catcgcgccc acggtgctcg agggccgctg ccccgagatg
     4201 ctgcaggatt tcatcgaagt gcgcggcctc ggcctgatca acatccgcac catcttcggc
     4261 gagaccgcgt gccggcggaa gatgcgcctg cgcctgatct gccacctcga ccgccgccaa
     4321 cccggtcccg acgacccctg ccggctgccg ctgcagcagg agacgcagga catcctcggc
     4381 gtgaaggtcg cccgcgtcgt gctgccggtg gccgcgggac gcaacctcgc ggtgctgctc
     4441 gaggccgcag tgcgctcgac catcctcaag ctgcgcggca tcgactccac ccaggaattc
     4501 atcgaccgcc aggcgcgcgc gctgcacgcc gaagaggcct accgcgccga ctgagccacc
     4561 cgcacttgcg gcacggcgcc gccggctgtt acgctcgatc gctttgcagc agcctactgg
     4621 ccggccatga gctccactcc cgattacctg gaacgcatcc tcaacgcgca ggtgtacgac
     4681 gtcgccgtcg agaccccgct cgacctcgcc accaacctgt cggtgcgcac gcacaaccgc
     4741 atctacttca agcgcgagga catgcagccc gtgttcagct tcaagatccg cggcgcctac
     4801 aacaagatgg cgaacctgtc gccgcaggcc ttgaagcgcg gcgtgatctg cgcctcggcg
     4861 ggcaaccacg cccagggcgt ggcgctgtcg gcggccaggc tgggcgtgcg cgcggtgatc
     4921 gtgatgccga gcaccacgcc gcagatcaag atcgacgcgg tcaaggcgcg cggcggcgag
     4981 gtcgtgctcg caggcgaatc ctattccgac gcctacacgc acgcgctcga gctcgagaag
     5041 agcgagaaac tcaccttcgt ccaccccttc gacgaccccg acgtgatcgc cggccagggc
     5101 acggtgggcc tggagatcct gcgcgcgcac ccgaagccga tccacgcggt gttctgctgt
     5161 gtcggcggcg gcgggctgat cgccggcgtg gcggcctaca tcaagcgcct gcgcccggag
     5221 acgaagatca tcggcgtcga ggcggtggac gccgacgcga tgacgcgctc gctcgccgcg
     5281 ggcaagcgca tcgcgctcga gcaggtcggc atcttcgccg acggcgccgc cgtcaaggag
     5341 gtcggtgccg agaccttccg cctgtgccag cagtatgtgg acgagatgat cgtggtcgac
     5401 aacgacgcca tctgcgccgc gatcaaggac gtcttcgagg acacccgctc catcctcgag
     5461 cccgccggcg cgc
//
LOCUS       DAOQNN010000214         5395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_8999_length_5395_cov_7.744007, whole genome shotgun sequence.
ACCESSION   DAOQNN010000214 DAOQNN010000000
VERSION     DAOQNN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8999_length_5395_cov_7.744007"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="PKV97_11215"
     CDS             complement(<1..1569)
                     /locus_tag="PKV97_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNB06490.1"
                     /translation="MLILAAALSAAPALAQTLPSAANPGALQQRRIDEEERRRQLEQL
                     ERREITDPVRREAAPPPAAPPAGEAVRFTVREIRFTESAILPAEALAELAGPYTGREV
                     SLAELRELAARIDAAYRERRVVTARAVIPPQDVSDGVVDIRLVEGRVGEIAVEGNAST
                     DADFITRRIGLVAGELVDLPVLEQDLRRFNRTQDAQLRADLAAGQRFGTSDLRILVEE
                     PPRHSLRLSLDNGGSEGSGEWRTGLQYTNRSLLGFRDELSLGVVGAKGQESYSAGYSF
                     PVNRSGGRLSLAAYKDHVEIVEGPLRDLDISGRSTGTIVALRQPVHLGERSRTDLVLG
                     SKRRETRNWISSVPLQETQTVDGHLGVEHQAADDHGYWLATYTLTGGRAKGFERSNYT
                     LGRAFVRRVHALAPGWSAQAGLNYQHSGHSPLPSGEQLQIGGEYSVRGYPVGVWSGDR
                     GFIANLELHHPLGELAASEGLPGVRATGFFFADHGRVTPDRAPNSLEPQHRQLGSAGW
                     GVRAAIGERVDARLT"
     gene            complement(1620..>5395)
                     /locus_tag="PKV97_11220"
     CDS             complement(1620..>5395)
                     /locus_tag="PKV97_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leukotoxin LktA family filamentous adhesin"
                     /protein_id="HNB06491.1"
                     /translation="RTDEYLYSQNGWFGITAAPVQDQYRVGTTANTLDPLLQGEFLRV
                     QSLTNPDAFVPTTTTVNGFRSVTQGASWKKCNWWTLCANATYYMQYFVTTGNKTTVTG
                     SVRADHPIPIEYIGFDTARIAIDSVGEVVLAGSLNNRSGTTSVDTDGHLSQGNATATV
                     GGRTIELSAGTGIGSTTPAGEPQQALRIRQLEGGVVHADTTSGSIHLDQVVGNLNVGR
                     IGSAGVDRVTLESAGDILAKDADALVQARRIDLVAENGRIGTLDEAGNGTPLRIRSGD
                     STSLDPDVLARHGLSARARGNIHLANEADPDPAAAGTYTGDLLLIGVESGAGDVRIRT
                     SGSAIDNNPYGSVDTRTEDQLAALWDELRLRVKADPDPNDGVAVGADAKAQEAVAAFA
                     NGRSNNYRLYWQLRGRQADGGAAYDPGYQYVPTATERRVLADSGMSDAEIAAFAANRS
                     AQYHALHAEVGGYTTAFDEDFRYVATDAERAAITRGSSWTEAQLLLSVGAGLIKKVTD
                     TVTTIQEPNVVGRKVTLIADGDIGAYGPDLVIDTTAGIDTLSKAERAALAAAERGDAQ
                     VDDPDNPRIVTLRQPQAVNLAVAPDGALSARAGGHVLIGAEQDLRIDRIVAGGETRIK
                     VAGGLSGLASATDAVNVKSGGRLILEAASAGIGGTLDPDGLFLTPFGIQLTSPDAGLI
                     LRAGGHAWVAAESGLFVDTVFALGDVRLDASGAILDFQLTEAPLAPELNLLARNVALA
                     SRTGAIGSSVNPFDVAVSHEDGRISASTARFGEGAHLNGPMGEFFNIANVTSGDAVAL
                     SSATFMFIDGRVTGPGPISLVSGASMVLTPFADIHATTLGVFLQASDLLMQESADGAD
                     AARIQVDVGTIDLRTVGHQAAPDAPRSGGDAVITGIFTGNPTESAILIDAFGSVLDGG
                     DKRLDIIARTAPAATLTIRAGGQIGGNPLEIDVLHFNADSGGLIHLDSPGSLAMGDVR
                     AEREIDIRAAGSISADSVTSVTEDVRLAADGGDLRVGAIAGRDVELGVQGGNAEIGSI
                     DARDLGLRVENGDARIGSIDARDVDLGARDRLDIDRLDVGRSLRLGGNDIVARVFASG
                     GAPIVGSIGGYGGGMASVADLTLSGPGGFHFDDIAARTGRLDLPLGTLQVDRFQVGER
                     MVVTNPQTRLLIDQQNFRLQGYDVQLYSGGAPFALALDRNLIATDSFVIDRRTSHEVR
                     GPDGPLASVVEYTERVLADIHLAPPAAGAPREDEEESLVRIAPTPVALGTEGQ"
BASE COUNT          675 a         1806 c         2068 g          846 t
ORIGIN      
        1 ggtgaggcgt gcgtccacgc gctcgccgat tgcggcgcgt acgccccagc ctgcactgcc
       61 gagctggcga tgctgcggtt cgagcgagtt gggtgcgcgg tcgggggtga cgcggccgtg
      121 gtcggcgaag aagaagccgg tggcgcgcac gccgggcagg ccttcgctcg cggcgagctc
      181 gccgagcggg tgatgcagtt ccaggttggc gatgaagccg cggtcgcccg accatacgcc
      241 caccgggtag ccgcgcacgc tgtactcgcc gccgatctgc agctgctcgc cgctcggcag
      301 cgggctgtgg ccgctgtgct ggtagttgag gccggcctgc gccgaccagc ccggggccag
      361 ggcgtggacg cggcgcacga aggcgcgccc gagcgtgtag ttgctgcgct cgaagccctt
      421 ggcgcgaccg ccggtgaggg tgtaggtggc gagccagtag ccgtggtcgt cggccgcctg
      481 gtgctccacg ccgaggtggc cgtccaccgt ctgcgtctcc tgcagcggta cgctgctgat
      541 ccagttgcgc gtctcgcgcc gtttgctgcc cagcaccagg tcggtgcgcg agcgttcgcc
      601 cagatgcacc ggttggcgca gcgcgacgat ggtgccggtg gagcggccgc tgatgtcgag
      661 gtcgcgcagc gggccctcga cgatctcgac gtggtccttg tacgccgcca ggctgaggcg
      721 gccgccgctg cggttcaccg ggaagctgta gcctgccgag tagctctcct gccccttcgc
      781 gccgaccacg cccaggctca gctcgtcgcg gaagccgagc aggctgcggt tggtgtattg
      841 caggccggtg cgccactcgc ccgagccctc gctgccgccg ttgtcgaggc tcaggcgcag
      901 gctgtggcgc ggcggctcct cgaccaggat cctgaggtcc gaggtgccga agcgctgccc
      961 cgccgccagg tccgcgcgca gctgcgcgtc ctgggtgcgg ttgaagcggc gcaggtcctg
     1021 ctccagcacc ggcaggtcga ccagctcgcc cgcgacgagg ccgatgcgcc gggtgatgaa
     1081 gtccgcgtcg gtgctggcgt tgccctctac cgcgatctcg cccacgcggc cttccaccag
     1141 gcggatgtcg acgacaccgt cggagacgtc ctgcggcggg atcaccgcgc gcgcggtgac
     1201 cacgcgccgt tcgcggtagg ccgcgtcgat gcgcgcggcg agctcgcgca gctcggcgag
     1261 gctcacctcg cgcccggtgt agggcccggc gagctcggcg agcgcctcgg cgggcaggat
     1321 ggccgactcg gtgaagcgga tctcccgcac cgtgaagcgc accgcctcgc ctgccggggg
     1381 ggcagccggc ggcggtgcgg cttcgcgccg gaccggatcg gtgatctcgc ggcgctcgag
     1441 ctgttcgagc tggcggcggc gctcctcctc gtcgatccgg cgctgctgca acgcgccggg
     1501 attcgccgcc gagggcaggg tctgggccag ggcgggggcc gccgagagcg ctgcagccag
     1561 gatcagcatg ggtttcacga ttgcgggctc tccactggag gggattggtc gtttcggcct
     1621 cattgtccct ccgttccgag cgcgacgggg gtgggggcga tgcggaccag gctctcttcc
     1681 tcgtcctcgc ggggcgctcc ggcagccggc ggggcgagat ggatgtcggc gagcacgcgc
     1741 tcggtgtact cgaccacgct cgccagcggg ccatcgggtc cgcgcacctc gtgcgaggtg
     1801 cgacggtcga tgacgaagct gtcggtggcg atcaggttgc gatcgagcgc cagggcgaag
     1861 ggggccccgc cggagtagag ctgcacgtcg tagccctgca ggcggaagtt ctgctggtcg
     1921 atcagcagcc gagtttgcgg attggtgacg accatgcgct cgccgacctg gaagcgatcg
     1981 acctgcaggg tgccgagggg caggtcgagc cggccggttc gagcggcgat gtcgtcgaag
     2041 tggaatccgc ccgggccgct cagcgtcagg tccgcgaccg aggccatccc gccgccatag
     2101 cccccgatgg agccgacgat cggcgcgcca ccgctggcga agacccgggc gacgatgtcg
     2161 ttgccgccca gtcgcaggct gcggccgacg tcgagacgat cgatgtcgag gcgatcgcgc
     2221 gcgccgaggt cgacgtcgcg ggcgtcgatc gaaccgatgc gagcgtcgcc gttctccacc
     2281 cggagcccga ggtcgcgcgc gtcgatcgag ccgatctccg cattgccacc ctgaacgccg
     2341 agttcgacgt cgcgtcccgc gatggcgccg acgcgcaggt cgccgccatc ggccgcgagc
     2401 cgtacgtcct cggtcacgct ggtgacggag tccgcactga tcgagccggc ggcgcggatg
     2461 tcgatctcgc gttcggcgcg tacgtcaccc atggccagcg aacccggcga gtcgaggtgg
     2521 atgaggccgc cggagtcggc gttgaagtgg agcacgtcga tctcgagcgg gttgccgccg
     2581 atctggccgc cggcacggat cgtcagggtg gcggcggggg cggtgcgagc gatgatgtcg
     2641 aggcgcttgt cgccaccgtc gagcacgctg ccgaaggcgt cgatgaggat cgcgctctcg
     2701 gtcgggttgc cggtgaagat gccggtgatg accgcgtcgc caccgctgcg gggcgcgtcg
     2761 ggtgcagcct ggtggcccac ggtgcgcagg tcgatggtgc cgacgtcgac ctggatgcgc
     2821 gccgcgtctg cgccgtccgc cgattcctgc atcagcaggt cggaagcctg caggaacacg
     2881 ccgagggtgg tggcgtggat gtcggcgaac ggggtcagca ccatgctggc gccggacacc
     2941 aggctgatcg ggcccggccc ggtgacgcgg ccgtcgatga acatgaaggt ggccgaggac
     3001 agcgcgacgg cgtcgcccga cgtgacattg gcgatgttga agaactcgcc catcggcccg
     3061 ttcaggtgcg caccttcgcc gaagcgagcg gtgctggcgg agatgcgccc gtcctcgtgc
     3121 gagaccgcga catcgaacgg attgacggac gagccgatcg cccccgtgcg cgaagccagc
     3181 gccacattgc gcgcgagcag attgagctcg ggcgccaggg gcgcctcggt gagctggaag
     3241 tcgaggatgg cgccgctggc gtcgaggcgt acgtcgccga gcgcgaacac cgtgtcgacg
     3301 aagaggcccg actcggccgc gacccaggcg tgcccaccgg cgcgcaggat caggccggcg
     3361 tcgggcgagg tgagctggat gccgaacggg gtcaggaaca gcccgtcggg gtccagcgtt
     3421 ccgccgatgc cggcgctggc ggcctccagg atgaggcggc cgccgctctt cacgttgacc
     3481 gcgtcggtcg cgctggcgag accgctgagg ccgcccgcca ccttgatccg cgtctccccg
     3541 ccggcgacga tgcggtcgat gcgcaggtcc tgctcggcgc cgatcaggac atggccgccc
     3601 gcccgcgcgc tcagcgcgcc gtcgggcgcg accgcgaggt tcaccgcctg cggctggcgc
     3661 agggtgacga tgcgcgggtt gtccgggtcg tcgacctggg cgtcgccgcg ttcggccgcg
     3721 gccagcgcgg cgcgctcggc cttgctcagg gtgtcgatgc cggcggtggt gtcgatcacc
     3781 aggtccggac cataggcgcc gatgtcgccg tcggcgatga gcgtgacctt gcggccgacg
     3841 acgttgggtt cctggatcgt ggtgacggtg tcggtgacct tcttgatcag gcctgcgccg
     3901 accgacagca gcagctgcgc ctcggtccaa ctcgagccgc gggtgatcgc ggcacgctcg
     3961 gcgtcggtgg cgacgtagcg gaaatcctcg tcgaaggcgg tggtgtagcc gccgacctcg
     4021 gcgtgcagcg cgtggtactg cgcgctgcgg ttggcggcga aggcggcgat ctcggcgtcg
     4081 ctcatgcccg aatcggcgag cacccggcgc tcggtggcgg tgggcacgta ctggtatccc
     4141 gggtcgtagg ccgcgccgcc gtcggcctgg cggccgcgga gctgccagta caggcggtag
     4201 ttgttgctgc gaccgttggc gaaggcggcg accgcctcct gtgccttggc atcggcgccc
     4261 accgcgacgc cgtcgttcgg gtcggggtcg gccttcacgc gcaggcgcag ctcgtcccac
     4321 agcgcggcga gctggtcctc cgtgcgcgtg tcgaccgagc cgtaggggtt gttgtcgatc
     4381 gcgctgccgc tggtgcggat gcgcacgtcg cccgcgcccg actccacgcc gatcagcagc
     4441 agatcgccgg tataggtgcc tgctgccgcc gggtcggggt cggcctcgtt ggcgaggtgg
     4501 atgttgccgc gcgcgcgcgc cgacaggccg tggcgggcga gcacgtcggg gtcgaggctg
     4561 gtgctgtcgc cgctgcggat gcgcagcggc gtgccgttgc cggcctcatc cagcgtgccg
     4621 atgcggccgt tctcggcgac caggtcgatg cgccgcgcct gcaccagggc gtcggcatcc
     4681 ttggcgagga tgtcgccggc ggactccagg gtcaccctgt ccaccccggc gctgccgatg
     4741 cggcccacgt tcaggttgcc caccacctgg tcgaggtgga tcgagccgct ggtggtgtcg
     4801 gcatggacca cgccgccttc gagctggcga atgcgcaggg cctgctgcgg ctcgcccgcg
     4861 ggcgtcgtgc tgccgatgcc ggtgccggcc gacagctcga tcgtgcgccc gccgacggtg
     4921 gcggtcgcgt tgccctgcga caggtggccg tcggtgtcga ccgaggtcgt gccgctgcgg
     4981 ttgttgaggc tgccggcgag caccacttcg ccgaccgagt cgatggcgat gcgggcggtg
     5041 tcgaagccga tgtactcgat cgggatcggg tggtcggcgc gtacgctgcc ggtgaccgtg
     5101 gtcttgttgc cggtggtgac gaagtactgc atgtagtacg tcgcattggc gcacaaggtc
     5161 caccagttgc acttcttcca gctcgccccc tgggtgacgg accggaagcc gttgaccgtg
     5221 gtcgtcgtgg gcacgaacgc gtccgggttg gtcaggctct gcacgcgcag gaactcgccc
     5281 tgcagcagcg gatcgagggt gttcgcggtg gtgccgaccc tgtactggtc ctgcaccggg
     5341 gccgcggtga tgccgaacca gccgttctgc gagtacaggt actcgtcggt gcgca
//
LOCUS       DAOQNN010000215         5316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9231_length_5316_cov_8.008363, whole genome shotgun sequence.
ACCESSION   DAOQNN010000215 DAOQNN010000000
VERSION     DAOQNN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9231_length_5316_cov_8.008363"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PKV97_11225"
     CDS             <1..636
                     /locus_tag="PKV97_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNB06492.1"
                     /translation="GATLAACRSALDREGGAACAANLAGGTHHAHRDFGSGFCVFNDA
                     AIAALAMRAEGRAGRIAIVDCDVHQGDGTATILADTPEVFTCSLHGAKNFPFRKQASD
                     LDVELPDDTGDEAYLAALDAALDTVFTRARPELVIYLAGADPYADDRLGRLALSIDGL
                     RRRDERVLSRCRAADVPVAVAMAGGYAQPIDDTVTIHFNTLCTAARVANAR"
     gene            802..1644
                     /locus_tag="PKV97_11230"
     CDS             802..1644
                     /locus_tag="PKV97_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HNB06493.1"
                     /translation="MTTPLYGVDFTSSPRRAKPITVAHGHLDGARVELVRFEHCVDWP
                     GFEALLARPGPWLGAFDFPFGLPREAIADLDWPQTWPALVRHCATLGKPAFRAALDAY
                     RESRPVGRRYAHRATDLPARSHSPLKLVNPPVGLMFLEGAPRLLAAGVRVPGMHDGDP
                     SRVAIEAYPGLLARRITPASYKSDERRKQNAERHAARKQIIDTLTAGPVSSQGASRPL
                     VLAATREQHQAMLDDASGDLLDAALALLQAAAAARLGPPHFGLPAGMDPLEGWIAGCG
                     TSNA"
     gene            1868..2983
                     /locus_tag="PKV97_11235"
     CDS             1868..2983
                     /locus_tag="PKV97_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNB06494.1"
                     /translation="MKRTVVAVALMSIGLSAAQAQEVVVKLGGAAPLTGNQSHLGKDL
                     ENGTRLAIEEANAKGVTIGGKKVKFELVGEDDQADPRTGTTVAQRLVDAGVKGVIGHL
                     NSGTSIPASRIYDQAGIPQVSPASTNPKLTLQNFSGVFRTIANDVQQGTGLGKYATGP
                     LGAKRVAIIDDRTAYGQGLADETAKAVKETGGEVVAREFTTDKATDFNAILTKIRATN
                     PEVVFYGGMDAQAGPMVRQMKQLGITAKFLTGDGGCSPEMIKLAGDGMSSSAYCSMPG
                     LPLEKMPGGADFRERYKKRFNADVQVYAPYAYDAAMAIITAMQKADSVEPSKYLPELK
                     KSNFPGVIGNVSFDANGDIKEGAVTIYNFKDGAWVPL"
     gene            3122..4054
                     /locus_tag="PKV97_11240"
     CDS             3122..4054
                     /locus_tag="PKV97_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB06495.1"
                     /translation="METLIQQFINGLVIGSVYALIALGYTMVYGILGLINFAHGDVLM
                     VGALVALQTMLFLMGMAPGMSPLVMLAIALAVAMVICMLLGFTMERLAYRRLRNAPRL
                     APLITAIGVSFLLQTLAMIIWGRNYHTFPQLISTTPLQPIPGVFVTPVQIAIVVVSAL
                     LMVGLLLLVNRTRIGRAMRATAENHRVASLMGVDTNAVIALTFVIGAALAAVAGVMFA
                     SNYGIAHYGMGFMPGLKAFTAAVLGGIGNLGGAMLGGVVLGLVESFGAGYIEHLTFGF
                     LNSSYQDIFAFIILGLVLIFRPTGLLGERVSDRA"
     gene            4068..5189
                     /locus_tag="PKV97_11245"
     CDS             4068..5189
                     /locus_tag="PKV97_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB06496.1"
                     /translation="MNELVAAVPWLGKRNPTAARWLIILVAIAAPFIAWQFGRSWVRI
                     LDFALLYMMLALGLNLVVGFAGLLDLGYIAFYAVGAYTFAFLASPHFDVHLPFWVILP
                     LGAAFAALAGVMLGFPVLRLRGDYLAIVTLGFGEIVRIFMLNLNYPVNITNGPQGINM
                     IDPINLFGWDLSRNIRIGEDFTIHSLFLYYYFFLAAVVASIIFIQRLQISRIGRAWAA
                     MRDDELAAKAIGINTRDMKLLAFALGATFGGVAGALFGAFQGFVSPESFSLMESIAVL
                     TMVVFGGMGNLAGAVVGAFVLALLPEILRDVAVPLQQTLFGHVLLDPEVLRMLLLSLA
                     MILMMLLRPAGMIPARARYCHDHHLSHHLNNTPKREAAK"
     gene            5186..>5316
                     /locus_tag="PKV97_11250"
     CDS             5186..>5316
                     /locus_tag="PKV97_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB06497.1"
                     /translation="MITLLEARNIGKRFGGLQALSDVSLTIRKGEVYGLIGPNGAGKT
                     "
BASE COUNT          814 a         1973 c         1667 g          862 t
ORIGIN      
        1 ggcgccacgc tggccgcctg ccgcagcgcg ctcgatcgcg agggcggcgc ggcctgcgcc
       61 gccaacctcg ccggcggcac gcaccacgca catcgcgact tcggctcagg cttctgcgtg
      121 ttcaacgacg ccgccatcgc cgcgctggcg atgcgggccg agggtcgcgc cggccgcatc
      181 gccatcgtcg attgcgacgt gcaccagggc gacggcaccg cgaccatcct cgccgacacg
      241 cccgaggtct tcacctgcag cctgcacggt gcgaagaact tccccttccg caagcaggcg
      301 agcgacctcg acgtcgaact gcccgacgac accggcgacg aggcctacct cgccgcgctc
      361 gatgcggcgc tcgacaccgt cttcacccgc gcccgccccg agctggtgat ctacctcgcc
      421 ggcgccgacc cctacgccga tgaccgcctc ggccgactcg cgctcagcat cgacggcctg
      481 cgccggcgcg acgaacgcgt gctcagccgg tgccgcgccg ccgacgtgcc ggtcgccgtc
      541 gccatggctg gcggctacgc gcagccgatc gacgacacgg tgacgatcca cttcaacacc
      601 ctctgcacgg cggcgcgggt ggccaatgcg cgctgacggc cgggttcaaa tccgcggcgc
      661 gcactgcgat ggtgccggaa cgcgagtacg tgccccggat cggcccgcgt tgcaccgggc
      721 atcgcgtccg tcccgacgcg gtgcggcctg cagcgccgaa gcaccgatca cgacgcattg
      781 cgtcgctgga gacatgcagc gatgacgacc ccactgtacg gcgtggactt cacctcctca
      841 ccccgccgcg ccaaaccgat caccgtggcc catggccacc tcgacggcgc gcgcgtggag
      901 ctcgtccgtt tcgagcactg cgttgactgg cccggcttcg aggccctgct cgctcgcccc
      961 ggcccctggc taggcgcctt tgacttcccg ttcggactgc cgcgcgaggc gatcgccgac
     1021 ctggactggc cgcagacctg gccagccctc gtgcgccatt gcgccacgct cggcaagccc
     1081 gcctttcgcg ccgcgctcga tgcctaccgc gaatctcgcc cggtcggtcg ccgctatgcc
     1141 catcgcgcca ccgacctccc cgcacgctcg cacagtccgc tgaagctggt gaatccgccg
     1201 gtcgggctga tgttcctgga aggggcgccg cgtctgcttg ccgctggcgt gcgtgtgccg
     1261 ggcatgcacg acggtgaccc gagccgcgtg gccatcgagg cttatcccgg gctgctcgcc
     1321 cgccgcatca cccccgcttc gtacaagagc gacgagcgcc gcaagcagaa cgcggaacgc
     1381 catgcggcac gcaagcagat catcgacacg ctcaccgccg gaccggtctc gtcgcagggc
     1441 gcgtcccgac cgctggtgtt ggccgccaca cgcgaacaac accaggccat gctcgacgac
     1501 gccagcggcg atcttctgga cgccgcgctc gcgctgctgc aggcggccgc ggctgcgcgc
     1561 cttggcccgc cgcacttcgg cctgcccgcc gggatggatc cgctggaggg gtggatcgcc
     1621 ggttgcggga caagcaacgc ttagcactcg gcgaccaccg gcttcgcacc atcgacgacg
     1681 tcgcatgacc cgggcatgcc tgcccaatac ggtcgcttgc ttggcatggc gcttgcttca
     1741 gcctcagggc aggacaagtc attcgcgcag ttaattcgcc cattcgcaac gcgttttttt
     1801 gggcacaata cggacgcgtt tcggcttcac ccaaacccct cccccgatca ccccggagat
     1861 ccagagcatg aagagaaccg tcgtcgcagt cgccctgatg agcatcggcc tcagtgccgc
     1921 ccaagcgcag gaagtggtcg tcaagctggg cggggccgcc ccgctcaccg gtaaccagtc
     1981 gcaccttggc aaggatctcg agaacggcac ccgcctcgcc atcgaggagg ccaacgccaa
     2041 gggcgtgacg atcggcggca agaaggtcaa gttcgagctg gtcggcgagg acgaccaggc
     2101 cgacccgcgc accggcacca cggtggcgca gcgcctggtc gatgccgggg tgaaaggcgt
     2161 gatcgggcat ctgaactcgg gcacctcgat tccggcctcg cgcatctacg accaggcggg
     2221 cattccgcag gtgtcgccgg cctcgaccaa cccgaagctg acgctgcaga acttcagcgg
     2281 cgtgttccgc accatcgcca acgacgtgca gcaaggtacc gggctcggca agtacgccac
     2341 cggcccgctc ggcgccaagc gcgtcgcgat catcgacgac cgcaccgcct acggccaggg
     2401 cctcgccgac gagaccgcga aggcggtgaa ggagacgggc ggcgaggtcg tcgcgcgcga
     2461 attcaccacc gacaaggcca ccgacttcaa cgccatcctc accaagatcc gcgccaccaa
     2521 ccccgaggtc gtcttctacg gcggcatgga cgcccaggcc ggcccgatgg tgcggcagat
     2581 gaagcagctc ggcatcaccg ccaagttcct caccggcgac ggcggctgca gccccgagat
     2641 gatcaagctc gccggcgacg gcatgtcgtc cagcgcctac tgctcgatgc cgggcctgcc
     2701 gctggagaag atgccgggcg gcgccgactt ccgcgagcgt tacaagaagc gcttcaacgc
     2761 cgacgtgcag gtgtatgccc cctacgccta cgacgccgcg atggccatca tcaccgcgat
     2821 gcagaaagcc gactcggtcg agccgtccaa gtacctgccc gagctcaaga agagcaactt
     2881 cccgggcgtg atcggcaacg tctccttcga cgccaacggc gacatcaagg aaggcgcggt
     2941 gacgatctac aacttcaagg acggcgcctg ggtgccgctg taagcgcctg aaccgccgcc
     3001 ggcgcacccg cttgcggcgc gccggcggcg cgcgacatcc ccgtttcgat ttgcccgccc
     3061 cggagaccct gcgcgtccgc cggggcgtgg cgcgcgactc cgcagacttc aagaaggcag
     3121 catggaaacc ctgatccagc aattcatcaa cgggctggtg atcggcagcg tctacgcgct
     3181 gatcgcgctc ggctacacga tggtgtacgg catcctgggc ctgatcaatt tcgcccacgg
     3241 cgacgtgctg atggtgggcg cgctggtcgc gctgcagacc atgctgttcc tgatgggcat
     3301 ggcacccggc atgtcgccgc tggtgatgct ggcgatcgcg ctcgcggtgg cgatggtgat
     3361 ctgcatgctg ctcggcttca ccatggagcg gctcgcctac cggcgattgc gcaacgcgcc
     3421 gcgcctggcg ccgctgatca ccgcgatcgg cgtgtccttc ctgctgcaga ccctggcgat
     3481 gatcatctgg ggccgcaact accacacctt cccgcaactc atctcgacca cgccgctgca
     3541 gccgatcccc ggcgtgttcg tcaccccggt gcagatcgcc atcgtggtgg tgtcggcgct
     3601 gctgatggtc ggcctgctgc tgctcgtcaa tcgcacccgc atcggccgcg cgatgcgcgc
     3661 gaccgccgag aaccaccgcg tcgccagcct gatgggggtc gacaccaacg cggtgatcgc
     3721 gctcaccttc gtcatcggtg ccgcgctcgc cgccgtggcc ggggtgatgt tcgccagcaa
     3781 ctacggcatc gcccactacg gcatgggctt catgccgggc ctcaaggcct tcaccgccgc
     3841 ggtgctgggc ggcatcggca acctcggtgg cgcgatgctc ggcggcgtcg tgctcgggct
     3901 ggtggagtcc ttcggcgcgg gctacatcga gcacctcacc ttcggcttcc tcaactcgtc
     3961 ctaccaggac atcttcgctt tcatcatcct cggcctggtg ctgatcttcc ggcccaccgg
     4021 actgctcggc gaacgcgtgt cggaccgggc ctgagcgagc acgcaccatg aacgaactcg
     4081 tcgccgccgt gccctggctg ggcaagcgca accccaccgc cgcgcgctgg ctgatcatcc
     4141 tcgtcgccat cgcggcgccc ttcatcgcct ggcagttcgg ccgcagctgg gtgcgcatcc
     4201 tcgacttcgc gctgctctac atgatgctgg cgctcggcct caacctggtg gtcggcttcg
     4261 ccggcctgct cgacctcggc tacatcgcgt tctatgcggt gggcgcctac actttcgcct
     4321 tcctggcctc gccgcacttc gacgtgcatc tgccgttctg ggtcatcctg ccgctgggcg
     4381 ccgccttcgc cgcgctcgcc ggcgtaatgc tcggctttcc ggtgttgcga ttacgcgggg
     4441 attacctcgc catcgtcacc ctcggtttcg gcgagatcgt gcgcatcttc atgctcaacc
     4501 tcaactaccc ggtcaacatc accaacggcc cgcagggcat caacatgatc gacccgatca
     4561 acctgttcgg ctgggacctg tcgcgcaaca tccgcatcgg cgaggacttc accatccact
     4621 cgctgttcct ctactactac ttcttcctcg ccgcggtggt cgcctcgatc atcttcatcc
     4681 agcgcctgca gatctcgcgc atcggccgcg cgtgggcggc gatgcgcgac gacgagctcg
     4741 ccgccaaggc gatcggcatc aacacccgcg acatgaagct gctcgccttc gccctcggcg
     4801 ccaccttcgg cggcgtggcg ggcgcgctgt tcggcgcctt ccagggcttc gtcagcccgg
     4861 agagcttcag cctgatggag tcgatcgcgg tgctgaccat ggtggtgttc ggcggcatgg
     4921 gcaacctcgc cggcgcggtg gtcggcgcct tcgtgctcgc gctgctgccc gagatcctgc
     4981 gcgacgtcgc cgtgccgctg cagcagaccc tgttcggcca cgtgctgctc gaccccgagg
     5041 tgctgcgcat gctgctgctg tcgctggcga tgatcctgat gatgctgctg cgcccggccg
     5101 gcatgattcc ggcgcgtgcg cgctactgcc acgatcacca cctgagccat cacctgaaca
     5161 acacgccgaa gcgggaggcc gcaaagtgat cacgctgctc gaagcccgca acatcggcaa
     5221 gcgcttcggc ggcctgcagg ccttgtccga cgtgtcgctg accatccgca agggcgaggt
     5281 ctacggcctg atcggcccca acggcgccgg caagac
//
LOCUS       DAOQNN010000216         5297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9298_length_5297_cov_9.166158, whole genome shotgun sequence.
ACCESSION   DAOQNN010000216 DAOQNN010000000
VERSION     DAOQNN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9298_length_5297_cov_9.166158"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PKV97_11255"
     CDS             <1..174
                     /locus_tag="PKV97_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB06498.1"
                     /translation="SELIDWLKLLVAEDIPPEALRSADGKAAIPPLHGALLKNEQDVH
                     LFERLVFMARHNR"
     gene            191..1582
                     /locus_tag="PKV97_11260"
     CDS             191..1582
                     /locus_tag="PKV97_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB06499.1"
                     /translation="MLRALLGRLFGRKRPAPSVAAGEAEGFRRLQRAAPLRAEGAPAS
                     PVEAGATFLCREAVLGRNQRITGYQFMLQAGTRAHIRHSSRNIHHVFAEVLVRSLVDA
                     DIGELLGERLAFIDLPDSFLSHPSLAALPARNMVIVPTVLPDAGAPEPAALRAAVTAL
                     RARGMRIGLIDPTVVGELAHLLPQAEFIVAQAEGLDARRGLKLGNIALEVAHDARLVV
                     RGLPSLDDFRFCCKLGASLFQGPFITSREDWSARDPGPNTARIGALIARLRADADTRE
                     LADLLKQDAALSLRLLRYINSAANALPQPVSSIEHALALLGRERLQRWLILLACTADK
                     AGERGGAVLETALVRGRMMELLGAGRPAAESDALFLVGLLSLIDVILQVPLDKALAPL
                     ALGAEVEASVRGDHGPYQALLALAVACERGEAGEGAAGDALRAAAAACGIAPEQASEC
                     HLQALSWAMQIQD"
     gene            1605..2783
                     /locus_tag="PKV97_11265"
     CDS             1605..2783
                     /locus_tag="PKV97_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB06500.1"
                     /translation="MLIDFFLHLKARKLPVSTREFLTVLEGLRDHVCGPSLDDFYYYA
                     RLCLVKDESLYDRFDQAFGEYFKGVTALPDLEADLPEDWLRAMVKKHLSPEERAKLEK
                     LGWDKLMEEFRKRLQEQKGRHQGGSKWIGTGGSSPFGNNGTHPEGIRVGGESAGNRTA
                     VKVWDKREFRNLDDSVELGTRNIKVALRRLRRFARQGAAEELDLDGTIAATARNAGWL
                     DLMMRPERHNAVKVLLFLDVGGSMDDHVKVCEELFSACRLEFKHLEYFYFHNCVYEGV
                     WRDNRRRHAERTPLLDVIHKYGSDYKLIFVGDATMSPYEILHPHGSVEHMNAEAGATW
                     LRRLLDAYPAAAWLNPEPERLWPYRQSIGILGQIMGERMYPMTLDGLARAMQALSRRH
                     "
     gene            2814..4346
                     /locus_tag="PKV97_11270"
     CDS             2814..4346
                     /locus_tag="PKV97_11270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB06501.1"
                     /translation="MEETELPCPFCNAHSTRPIALPDGLWSACCADCGARGPIAPDAR
                     TALARWEQATRDGELLRTVIDESPDIILLKDWDGRFLLGNAALARLYDTTPEQLVGKD
                     DGAFNPNREQVDFYLENVRAVMRGGRTQIVEESSTNAETGEVHYFHSIKKPLRGPAGE
                     PRILVIAHDITDLRRAYRVIEERERSYAYAMEAAGEGIWDWDIDAGTVTHNRRWCELL
                     GYAADTLVHPIAAFADSLHPDDHDEVMGLLDRALAGVGSYEHEHRMRRPDGSVLWVLD
                     RGRVVERAPDGRPLRMAGTVTDVSPRKHNEFVLALATDALAHANAELERKVAERTADL
                     ARANEELARLARRDALTGLPNRLAVDERLHEESRRQPGPCGTGCAVLMIDIDSFKPIN
                     DTHGHATGDAVLALVAGLLAATVREADFVSRYGGEEFLVLLPDTALEGARTVAEKLRT
                     VVAETAHPVAGRITISIGLAMAAPGTGELATVVAQADAALYEAKRSGRNRLVIAPPPT
                     AV"
     gene            complement(4473..4976)
                     /locus_tag="PKV97_11275"
     CDS             complement(4473..4976)
                     /locus_tag="PKV97_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB06502.1"
                     /translation="MAETQQKVKKPAAQATPLSVLQRFRILIRTAQRHSQWIERQSGV
                     TGAQLWALQELREAPGLRVGELANRMALHQSTTSNMVDRLETSGLIRKERTSADQRVV
                     RLHLTDDGLALLSRAPSPARGVLPEALRLMDDEGLASLQSELEKLLRQIKNLDEGFGM
                     QPLPFTE"
BASE COUNT          815 a         2012 c         1727 g          743 t
ORIGIN      
        1 tccgagctga tcgactggct caagctgctg gtggccgagg acatcccgcc cgaggcgctg
       61 cgcagcgccg acggcaaggc ggcgatcccg ccgctgcacg gcgccctgct caagaacgag
      121 caggacgtgc acctcttcga gcgcctggtc ttcatggccc gccacaaccg ctgatccggc
      181 gcacgcatcc atgctccgcg ccctgctcgg ccggctcttc ggccgcaagc gccccgcccc
      241 ctccgtcgca gcgggcgagg cggagggttt ccgccgcctc cagcgcgcgg cgccgctgcg
      301 cgccgagggg gcccccgcga gccccgtcga ggccggcgcc accttcctgt gccgcgaagc
      361 ggtgctcggg cgcaaccagc gcatcaccgg ctaccagttc atgctgcagg cgggcacgcg
      421 cgcgcacatc cgccacagca gccgcaacat ccaccacgtc ttcgccgaag tgctggtgcg
      481 cagcctggtc gacgcggaca tcggcgagct gctcggcgaa cgcctggcct tcatcgacct
      541 gcccgattcc ttcctgtccc accccagcct cgccgccctc ccggcgcgca acatggtgat
      601 cgtgccgacc gtgctccccg atgccggcgc tcccgagccc gccgcgctgc gcgccgccgt
      661 gaccgcgctg cgcgcgcgcg gcatgcgcat cggcctgatc gatccgaccg tggtgggcga
      721 gctcgcccac ctgctgccgc aggccgagtt catcgtcgcc caggccgagg gcctcgacgc
      781 ccgccgcggc ctcaagctcg gcaacatcgc cctcgaggtg gcgcacgacg cgcgcctggt
      841 ggtgcgcggc ctgccctcgc tcgacgactt ccgcttctgc tgcaagctcg gcgccagcct
      901 cttccagggc cccttcatca ccagccgcga ggactggagc gcgcgcgacc ccggccccaa
      961 caccgcacgc atcggcgccc tgatcgcccg cctgcgcgca gatgccgaca cccgcgagct
     1021 cgccgacctg ctcaagcagg acgctgcgtt gtcgctgcgc ctgctgcgct acatcaactc
     1081 cgcggcgaac gcgctgccgc agccggtgtc ctcgatcgaa cacgcgctcg cgctgctcgg
     1141 ccgcgagcgc ctgcagcgct ggctgatcct gctcgcgtgc accgcggaca aggcgggaga
     1201 acgcggcggc gcggtgctgg agaccgcgct ggtacgcggg cgcatgatgg agctgctcgg
     1261 tgccggccgc cctgcggccg agtccgacgc gctcttcctg gtcggcctgc tgtcgctgat
     1321 cgacgtgatc ctgcaggtgc cgctcgacaa ggcgctcgcc ccgctcgcgc tgggagcgga
     1381 ggtcgaagcc tcggtgcgcg gcgaccatgg cccctatcag gccctgctcg cgctcgcggt
     1441 ggcgtgcgag cgtggagagg ccggagaggg tgcggcgggc gacgcgctcc gcgccgccgc
     1501 cgcggcctgc ggcatcgccc ccgagcaggc ctccgaatgc cacctgcagg ccctgtcctg
     1561 ggcgatgcag atccaggact gaccgcgcac gaagggaaga ccccatgctc atcgacttct
     1621 tcctgcacct caaggcacgc aagctgccgg tgtccacacg cgagttcctc accgtgctcg
     1681 agggcttgcg cgaccacgtc tgcgggccct cgctcgacga cttctactac tacgcgcggc
     1741 tgtgcctggt gaaggacgaa tcgctctacg accgcttcga ccaggccttc ggcgaatact
     1801 tcaagggcgt caccgccctg cccgacctgg aggccgacct gcccgaggac tggctgcgcg
     1861 cgatggtgaa gaagcacctc tcgcccgagg agcgcgccaa gctcgagaag ctcggctggg
     1921 acaagctgat ggaggaattc cgcaagcgcc tgcaggaaca gaagggacgc caccagggcg
     1981 gcagcaagtg gatcggcacc ggcggcagct cgcccttcgg caacaacggc acccaccccg
     2041 agggcatccg cgtgggtggc gagtccgccg gcaaccgcac ggcggtcaag gtgtgggaca
     2101 agcgcgagtt ccgcaacctc gacgattcgg tcgagctcgg cacgcgcaac atcaaggtcg
     2161 cgctgcgtcg cctgcgccgc ttcgcccgcc agggcgcggc cgaggagctc gacctcgacg
     2221 gcaccatcgc cgccaccgcg cgcaacgccg gctggctcga cctgatgatg cgccccgaac
     2281 gccacaacgc ggtcaaggtg ctgctcttcc tcgacgtcgg cggctcgatg gacgaccacg
     2341 tcaaggtctg cgaggagctc ttctccgcct gccggctcga gttcaagcac ctcgagtact
     2401 tctacttcca caactgcgtg tacgagggcg tttggcgcga caaccgccgc cgccacgccg
     2461 agcgtacgcc gctcctcgac gtgatccaca agtacggctc ggactacaag ctcatcttcg
     2521 tcggcgatgc caccatgagc ccctacgaga tcctgcatcc gcacggctcg gtcgagcaca
     2581 tgaacgccga ggccggcgcc acctggctgc gccgcctgct cgacgcctac ccggccgcgg
     2641 cctggctcaa ccccgagccg gagcgcctgt ggccctaccg ccagtcgatc ggcatcctcg
     2701 gccagatcat gggcgagcgc atgtacccga tgaccttgga cggcctggcg cgcgcgatgc
     2761 aggccctgtc ccgccgccac tgatcgaggc atcgcacccc cgctccagcc gtcatggaag
     2821 agaccgaact cccctgcccg ttctgcaacg cccacagcac ccggccaatc gcgctccccg
     2881 acgggctctg gagcgcgtgc tgcgccgact gcggcgcacg cggcccgatc gcgcccgacg
     2941 cccgcacggc gctggcgcgc tgggaacagg cgacgcgcga cggcgagctg ctgcgcacgg
     3001 tgatcgacga gtcgcccgac atcatcctgc tcaaggactg ggatggccgc ttcctgctcg
     3061 gcaacgccgc gctcgcacgc ctgtacgaca ccacgccgga gcaactcgtc ggcaaggacg
     3121 acggcgcctt caacccgaac cgcgagcagg tggatttcta cctggagaac gtgcgcgccg
     3181 tgatgcgcgg tggtcgcacg cagatcgtcg aggagagctc caccaatgcc gagaccggcg
     3241 aggtgcatta cttccactcg atcaagaagc cgctgcgcgg tcccgcgggc gagccgcgca
     3301 tcctcgtgat cgcgcacgac atcaccgacc ttcgacgtgc ataccgcgtc atcgaggagc
     3361 gcgagcgcag ctacgcctat gccatggagg ctgccggcga aggcatctgg gactgggaca
     3421 tcgatgccgg cacggtcacc cacaaccggc gctggtgcga gctgctcggt tacgccgccg
     3481 acaccctggt gcacccgatc gccgcattcg cggacagcct gcacccggac gatcacgacg
     3541 aggtgatggg cctgctcgac cgggccctcg ccggcgtggg cagctacgag cacgagcacc
     3601 gcatgcgtcg ccccgacggc agcgtgctgt gggtcctcga ccgcgggcgt gtcgtcgagc
     3661 gcgcacccga cggccgcccg ctgcgcatgg cgggcacggt caccgacgtc agcccgagaa
     3721 aacacaacga gttcgtgctc gcgctcgcga ccgacgcgct cgcgcacgcc aacgccgagc
     3781 tcgagcgcaa ggtggccgaa cggaccgccg atctcgcccg cgccaacgaa gagctcgccc
     3841 gcctcgcccg ccgcgacgcg ctcaccggac tgcccaaccg actcgccgtc gacgaacgcc
     3901 tgcacgagga gtcgcgccgg cagccgggac cgtgcggcac cggctgcgct gtgctgatga
     3961 tcgacatcga ctcgttcaag ccgatcaacg acacgcacgg ccacgccacc ggcgacgcgg
     4021 tgctggccct cgtcgccggc ctgctcgccg ccacggtgcg cgaggctgac ttcgtgtcgc
     4081 gctacggcgg cgaggagttc ctggtactgc tgcccgatac cgccctcgag ggcgcgcgca
     4141 ccgtcgccga gaagctgcgc accgtcgtcg ccgagacggc gcatcccgtg gccgggagga
     4201 tcaccatctc gatcggcctg gcgatggccg cacccggcac cggcgagctg gcgaccgtgg
     4261 tcgcgcaggc cgacgccgcg ctctacgagg ccaagcgcag cggtcgcaac cggctggtga
     4321 tcgcgccccc gccgaccgcc gtctgagccc cgtacggccg cgcggcgaca aaaaaatggc
     4381 ggcctcgcgg gccgccgaat ctgctgtccg cacggaggcg gtcacttcat ctgaagtacg
     4441 gtcgaggcgg accgccccgg gcggaccgcc gttcattcgg tgaagggaag cggctgcatg
     4501 ccgaagcctt cgtcgagatt cttgatctgg cgcagcagct tctcaagctc gctctgcagg
     4561 ctggcgaggc cctcgtcgtc catcagccgc aaggcctcgg gcagcacacc ccgcgccggc
     4621 gaaggcgccc tcgacagcag cgcgagcccg tcgtcggtga ggtggaggcg caccacgcgc
     4681 tggtcggcgc tcgtgcgctc cttgcggatc agccccgagg tctcgagccg gtccaccatg
     4741 ttcgaggtgg tggactggtg aagcgccatc cggttcgcga gctcgcccac gcgcaggccg
     4801 ggcgcctcgc gcagctcctg cagcgcccac agctgggcgc cggtgacgcc gctctggcgc
     4861 tcgatccact gggagtggcg ctgcgcggtg cggatcagga tccggaaacg ctgcaggacc
     4921 gacagggggg tcgcctgagc cgccggcttc ttcactttct gttgcgtttc cgccattcga
     4981 gtctcgctgg aactatattt ccacaaacaa tgattgtcaa aaaaacatca ctccgctcac
     5041 gcttgcgtat ggtagcggta tcgttcgttc ttcgcatgtg cagcattgca gaaaggcctc
     5101 tggaaatgct gctgtcgtcc actccgccag gaaggaatcc cgcatggcag cacagccacc
     5161 gatgagcccc gagcggaacc ccgctgctcc tgcacaagcc gacggcgcgc gccccgctgc
     5221 gccgcctccc gagccgcccg actcgcccat cgagccgccc ggatcagcgg tcgcgccccg
     5281 caccgccgca cgcgggc
//
LOCUS       DAOQNN010000217         5277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9355_length_5277_cov_9.700498, whole genome shotgun sequence.
ACCESSION   DAOQNN010000217 DAOQNN010000000
VERSION     DAOQNN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9355_length_5277_cov_9.700498"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1972
                     /locus_tag="PKV97_11280"
     CDS             <1..1972
                     /locus_tag="PKV97_11280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB06503.1"
                     /translation="LLVKAGRHPGASRAILSHSGAPMGEDAVFYAALRRAGVIRLYNM
                     GQLFAAANALFSHFRPRGNRLAIITNGGGPGVMAADRAADIGIPLAEFAESTVEKLNA
                     CLPSGWSHGNPVDILGDAGPERYRAALKAVLEGPNVDGVLVMLTPQAVTDPSGVADVV
                     IELEKTADKPVLVCWMGEELVAEARAKFSAAGIPHFRTPEPAVELFSHISAYYQNQKL
                     LMQTPSSLSHLPPPSIESARLVIEMALSERRKKLNEMESKALLAAFRIPIAQTVVARS
                     AAEAMVLSAEIGLPVVMKIDSPNIVHKSEVGGVRLNIRSLAAVRSTYQEILDEVKRVQ
                     PEAVINGIAIEPMIQKRNGRELVVSVRRDPVFGPAITFGEGGNLVEENRDVAVALPPL
                     NSFLVKDMIRSTRISTRLGEFRNMPAVDMNALELVLLRISEMVCELPWITAMEINPLI
                     VDENGVVAVDANISVENVSPTADRYDHVAIHPYPSHLISTWTVPDGTTVTIRPIKPED
                     AELEVDFVRRLSAETKYYRFMNTMRELPPAMVARLTQIDYDREMAFVATLEVEGVENE
                     IGVCRYAVNPDGESCEFAVVVADDWQHRGLARKLMGVLIETARSRGIQYMNGVFLANN
                     ERMLRFVQKLGFVLSNDPEDSSVKLGILALQD"
     gene            1976..2824
                     /locus_tag="PKV97_11285"
     CDS             1976..2824
                     /locus_tag="PKV97_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HNB06504.1"
                     /translation="MPATIEPIEFHGQPALRLATASGARAVVALHGAQVLSWAPPGGE
                     ERLYLSPKAVFDGHSAVRGGIPVCFPQFADLGPLPAHGFARNRRWSLTTERSGKDFAL
                     AGLTLADDEATRALWPYAFSAELSILIEDSRLDLELEITNTGDEAFEFTAALHTYLAV
                     REVEESRIEGLHGFSYRDKTDADRIQRDSGDALVIESETDRVYHEVNRPLLLREYHRS
                     LGVNADGFPDVVVWNPWIERSAAIADLPDDGFRRMLCIEAAAARQPVRVQPGESWWGR
                     QTLVAL"
     gene            3048..>5277
                     /locus_tag="PKV97_11290"
     CDS             3048..>5277
                     /locus_tag="PKV97_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNB06505.1"
                     /translation="MLPPIEYRIAEELGVSPRQVIAAVQLIDDGATVPFIARYRKEVT
                     GGLDDTQLRTLEERLGYLRELEDRRATVLASIEEQGKLTAELRAEVEDADTKQRLEDL
                     YLPYKPKRRTKAQIAREAGIGPLAETLLADPTLTPEVEAEKYINAEAGFADTKAVLDG
                     ARQILMEHFAEDATLLGELRSYLHEHGRVRSSVIEGKENEGAKFRDWFDFAEPIATMP
                     SHRALALLRGRNEGVLRLALDVERPDPDAPHPCEGRIAVRFGIRSQGRPGDRWLAETA
                     RWAWSVKISIHLELELMSELRERAEEEAIRVFARNLHDLLLAAPAGTRATIGLDPGIR
                     TGVKVAVVDATGKLVDTATVYPFEPRRDRESSIATIAALAKKHAVELIAIGNGTASRE
                     TDALVAELMKRYPELQLTKIVVSEAGASVYSASELAAREFPELDVSLRGAVSIARRLQ
                     DPLAELVKIDPKSIGVGQYQHDVNQGRLAKSLDAVVEDCVNAVGVDVNTASAPLLARI
                     SGLNATLAGNIVEYRNTRGPFRSRSALKDVPRLGPKTFEQAAGFLRIPSGDNPLDASS
                     VHPEAYPVVERILARVRKSVRELMGNGGFLKSLKPEEFTDERFGLPTVQDILAELEKP
                     GRDPRPEFRTATFREGVETLKDLEPGMLLEGVVTNVTNFGAFVDIGVHQDGLVHISAL
                     SNTFVKDPHSVVKAGQVVKVKVLEVDIPRHRIALTMRLGDEVTAKRGESGARRDEGAP
                     RRG"
BASE COUNT          863 a         1925 c         1736 g          753 t
ORIGIN      
        1 gctgctggtc aaggccggcc gccaccccgg cgcctcgcgc gcgatcctgt cgcacagcgg
       61 cgcgccgatg ggcgaggacg cggtgttcta cgccgcgctg cgtcgcgccg gcgtgatccg
      121 cctgtacaac atgggccagc tcttcgccgc ggccaacgcg ctgttctcgc acttccgccc
      181 gcgcggcaac cgcctcgcga tcatcaccaa cggcggcggc ccgggcgtca tggcggccga
      241 ccgcgcggcc gacatcggca tcccgctcgc ggagttcgcc gagagcacgg tcgagaagct
      301 caacgcctgc ctgcccagcg gctggtcgca cggcaacccg gtggacatcc tcggcgacgc
      361 cggcccggaa cgttatcgcg cggcgctcaa ggcggtgctc gaaggcccca acgtcgacgg
      421 cgtgctggtg atgctgaccc cgcaggcggt gaccgacccg agcggcgtcg ccgacgtcgt
      481 gatcgagctc gagaagaccg ctgacaagcc ggtgctggtg tgctggatgg gcgaggagct
      541 cgtcgccgag gcgcgcgcca agttcagcgc cgcgggcatc ccgcacttcc gcacgccgga
      601 gccggcggtc gagctcttca gccacatctc ggcctactac cagaaccaga agctgctgat
      661 gcagacgccg tcctcgctgt cgcacctgcc gccgccctcg atcgagagcg cgcgcctggt
      721 gatcgagatg gccctgtccg agcgccgcaa gaagctcaac gagatggagt cgaaggcgct
      781 gctcgcggcc ttccgcattc cgatcgcgca gaccgtggtc gcgcgcagtg ccgccgaggc
      841 gatggtgctc tcggccgaga tcggcctgcc ggtggtgatg aagatcgact cgccgaacat
      901 cgtgcacaaa tccgaggtgg gcggcgtgcg cctgaacatc cgcagcctcg ccgcggtgcg
      961 ctcgacctat caggagatcc tcgacgaggt caagcgcgtg cagcccgagg cggtgatcaa
     1021 cggcatcgcg atcgagccga tgatccagaa gcgcaacggc cgcgagctgg tggtgagcgt
     1081 gcgccgcgac ccggtgttcg gccccgcgat caccttcggc gagggcggca acctggtcga
     1141 ggagaaccgc gacgtcgcgg tggccctgcc gccgctcaac agcttcctcg tcaaggacat
     1201 gatccgctcg acccgcatct ccacccgcct cggcgagttc cgcaacatgc cggcggtgga
     1261 catgaacgcg ctcgagctgg tgctgttgcg catctccgag atggtctgcg agctgccgtg
     1321 gatcaccgcg atggagatca acccgctgat cgtggacgag aacggcgtgg tcgcggtcga
     1381 cgcgaacatc agcgtggaaa acgtgtctcc gacagcggac cgctacgacc acgtcgcgat
     1441 ccacccgtat ccgtcgcacc tgatcagcac ctggaccgtg cccgacggca ccacggtgac
     1501 gatccgtccg atcaagccgg aggacgccga gctcgaggtc gacttcgtgc gccgcctgtc
     1561 ggccgagacc aagtactacc gcttcatgaa caccatgcgc gagttgccgc ccgcgatggt
     1621 cgcgcgcctg acccagatcg actacgaccg cgagatggcc ttcgtcgcca ccctcgaggt
     1681 cgagggcgtc gagaacgaga tcggcgtttg ccgctacgca gtgaacccgg acggcgagtc
     1741 ctgcgaattc gccgtggtgg tggccgacga ctggcagcac cgcggcctcg cccgcaagct
     1801 catgggcgtg ctgatcgaga cggcgcgcag ccgtggcatc cagtacatga acggggtctt
     1861 cctcgccaac aacgagcgca tgctcaggtt cgtgcagaag ctcgggttcg tgctcagcaa
     1921 cgatccggaa gacagctcgg tcaagctggg cattctggcg ctgcaggact gagcgatgcc
     1981 cgcgacgatc gagcccatcg agttccacgg ccagccagcg ctgcgcctgg ccaccgcttc
     2041 gggtgcacgc gcggtggtgg cgctgcacgg cgcgcaggtg ctgtcgtggg cgccaccggg
     2101 tggcgaggag cgcctgtacc tgagcccgaa ggcagtgttc gacgggcaca gcgccgtccg
     2161 tggcggcatc ccggtgtgct tcccgcagtt cgccgacctc ggccccctgc ccgcgcacgg
     2221 cttcgcgcgc aaccggcggt ggtcgctcac gaccgagcgc agcggcaagg acttcgccct
     2281 cgcggggctg acgctcgccg acgacgaagc cacccgcgcg ctgtggccgt acgcgttttc
     2341 ggccgagctg tcgatcctga tcgaggacag ccggctcgac ctcgaactcg agatcaccaa
     2401 caccggcgac gaggccttcg agttcaccgc cgcgctgcac acctacctcg cggtacgcga
     2461 ggtcgaggaa tcgcgcatcg aaggcctgca cggcttctcc tatcgcgaca agaccgacgc
     2521 cgaccgcatc cagcgcgata gcggggacgc cttggtgatc gagtccgaga ccgaccgcgt
     2581 ctatcacgag gtaaaccgcc cgctgctgct gcgcgaatac caccgcagcc tgggcgtcaa
     2641 tgccgacggt ttccccgacg tggtggtgtg gaacccctgg atcgagcgca gcgccgcgat
     2701 cgcggacctg ccggacgacg gcttccgccg catgctctgc atcgaggccg ccgccgcccg
     2761 ccagccggta cgcgtgcagc ccggcgaatc gtggtggggg cggcagacct tggtcgccct
     2821 ctgagcctgc gtcgcgcacg cacgagccgc cgcagcctcg ctgccttctg ccggagcgac
     2881 gcgagtcgca ctcacggcgc ccgaagtccg cgccgccgtc gccggatcgc ccgctcacga
     2941 ctcgcttcgc cccgcaacgg ccgccacacc ggtccgcggg ttgctctccc cgcgccgcct
     3001 gttgcactat ccgggcctta tcttctgcgc ttcaccgcct cttccccatg ctccccccga
     3061 tcgaataccg catcgccgaa gagctcggcg tctcgccgcg ccaggtcatc gccgccgtcc
     3121 agctcatcga cgatggcgcc accgtgccct tcatcgcccg ctaccgcaag gaagtcaccg
     3181 gtggcctgga cgacacgcag ttgcgcacgc tggaggagcg cctgggctac ctgcgcgagc
     3241 tcgaagaccg ccgcgccacc gtgctggcct cgatcgagga gcagggcaag ctcaccgccg
     3301 agctgcgtgc agaggtcgag gacgccgaca ccaagcagcg cctcgaggac ctctaccttc
     3361 cctacaagcc caagcgccgc accaaggcac agatcgcgcg cgaagccggc atcggcccgc
     3421 tggccgagac attgctcgcc gacccgaccc tgacgcccga agtcgaagcc gaaaagtaca
     3481 tcaacgccga ggccggtttt gccgacacca aggcggtgct cgacggcgcg cgccagatcc
     3541 tgatggagca cttcgccgag gacgccaccc tactcggcga gctgcgcagc tacctgcacg
     3601 aacatggccg ggtgcgctcg agcgtgatcg agggcaagga gaacgaaggc gccaagttcc
     3661 gcgactggtt cgacttcgcc gaaccgatcg ccaccatgcc ctcgcaccgt gcgctggcgc
     3721 tgctgcgcgg gcgcaacgaa ggcgtgctgc gcctggcact cgacgtcgag cgccccgacc
     3781 ccgacgcccc gcatccctgc gaaggccgca tcgccgtgcg cttcggcatc cgcagccagg
     3841 gccgcccggg cgaccgctgg ctcgccgaga ccgcgcgctg ggcgtggagc gtgaagatct
     3901 ccattcacct cgaactcgag ctgatgagcg agctgcgcga gcgcgccgag gaagaggcga
     3961 tccgcgtctt cgcgcgcaac ctccacgacc tgctgctcgc cgcccccgcc ggcacgcgcg
     4021 ccaccatcgg cctcgacccc ggaatccgca ccggagtgaa ggtcgcggtg gtggatgcca
     4081 ccggcaagct ggtcgacacc gccaccgtgt atcccttcga gccgcgccgc gaccgcgaat
     4141 cgtcgatcgc caccatcgcc gcgctggcga agaagcatgc ggtcgagctg atcgccatcg
     4201 gcaacggcac cgcctcgcgc gagaccgacg cgctggtggc cgagctgatg aagcgctacc
     4261 cggagctgca gctgaccaag atcgtcgtct cggaggccgg cgcctcggtt tattccgcgt
     4321 ccgaactcgc cgcacgcgag ttccccgagc tcgacgtcag cctgcgcggc gcggtttcga
     4381 tcgcccgccg cctgcaggat ccgctcgccg agctggtcaa gatcgatccc aagtcgatcg
     4441 gcgtcggcca gtaccagcac gacgtcaacc agggccgcct ggcgaagagc ctggacgccg
     4501 tggtggaaga ctgcgtgaac gcggtcggcg tggacgtgaa caccgcgtct gcaccgctgc
     4561 tcgcacgcat ctcgggtctg aacgccacgc tcgccggcaa catcgtcgag tatcgcaata
     4621 cccggggccc cttccgcagc cgcagcgcat tgaaggacgt gccccgcctc ggccccaaga
     4681 ccttcgagca ggccgcgggc ttcctgcgca tccccagcgg cgacaacccg ctcgacgcct
     4741 cctcggtgca ccccgaggcc tacccggtgg tcgagcgcat cctcgcgcgc gtgcggaaga
     4801 gcgtgcgcga actgatgggc aacggcggct tcctcaagag cctgaagccg gaggagttca
     4861 ccgacgagcg cttcgggctg cccaccgtgc aggacatcct cgccgaactg gaaaagcccg
     4921 gccgcgaccc gcgccccgag ttccgcaccg cgaccttccg cgagggcgtg gagaccttga
     4981 aggacctcga gcccggcatg ctgctcgaag gggtggtcac caacgtcacc aacttcggcg
     5041 ccttcgtcga catcggcgtg catcaggacg gcctggtgca catctccgcg ctgtccaaca
     5101 ccttcgtcaa ggacccgcac agcgtggtca aggcgggtca ggtggtgaag gtgaaggtgc
     5161 tggaggtgga catcccgcgc caccgcatcg cgctcaccat gcgcctgggc gacgaggtga
     5221 ctgcgaagcg cggcgagtcc ggcgcccggc gcgacgaggg tgcgccgcgg cgcgggg
//
LOCUS       DAOQNN010000218         5267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9391_length_5267_cov_8.027437, whole genome shotgun sequence.
ACCESSION   DAOQNN010000218 DAOQNN010000000
VERSION     DAOQNN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9391_length_5267_cov_8.027437"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..215)
                     /locus_tag="PKV97_11295"
     CDS             complement(3..215)
                     /locus_tag="PKV97_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06506.1"
                     /translation="MNKTQTEPDMIKRQTIAAMLTTTAIAFSIATPAQAARLLEAGPF
                     DTVSACSGDKPATATPGERKDKPAQT"
     gene            complement(212..1621)
                     /locus_tag="PKV97_11300"
     CDS             complement(212..1621)
                     /locus_tag="PKV97_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB06507.1"
                     /translation="MHATAAAAPARLLAVDDDADLLRLLAMRLQATGYDVATADSVAA
                     ARSRLGVERFDLVVSDVRLPDGDGLALFEDIRRQHPALPVILLTAHGTIPDAVEATAL
                     GVAGYLTKPFDSQALLQGIRQALQRAGPTHARSGLRAEAPASAGDTAWRAAILSRSSR
                     MQAVLDEARLVAAADASVLIRGDSGTGKELLARAIHLASPRAAAPFVAINCGAIPEPL
                     LESELFGHVRGAFTGATSAHQGLVQAAHGGTLFLDEIGDMPPALQVKLLRVLQERAVR
                     PVGATRAQAVDLRIVSATHRDLDAALAAGQFRADLYYRIDVVSLQLPTLAERREDIPL
                     LAGHFLRSLAHKYGRRLTGFAPEALEALATAPWPGNVRQLHNVVEQACALATTALIPR
                     ALVERALRVQPVEALGYAEAKRRFERNYLIQLLKLTAGNVSDAARLAERNRTEFYRLL
                     QRHDLTPELFRGAGPDVEQ"
     gene            complement(1636..2103)
                     /locus_tag="PKV97_11305"
     CDS             complement(1636..2103)
                     /locus_tag="PKV97_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06508.1"
                     /translation="MLPATLALLAGCADLPARQAAEDDTSAAALAHEAAQQRLHTAVQ
                     AAQPGQERLATARRALESLLADDRAQARALHPYARALLEQLRVRQQVAALAEHLRRQL
                     DERARASDAQALELDALRRQNAALQRQLDALAEIERELNSPGLPVRPRTGLPE"
     gene            complement(2174..3634)
                     /locus_tag="PKV97_11310"
     CDS             complement(2174..3634)
                     /locus_tag="PKV97_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB06509.1"
                     /translation="MHHLSFRSILLASFLLITASLAAAVAGGWRGMETVVRAIQEDKQ
                     RALALSAAARQLRERSVNLERSARQYEVLGEPALARRFEATLAEALPALALLESAGPA
                     LASAGAAWRACADRIAAWLASLAIPGPTDGVREQPMPATAAGRGDGLATADDFRHLGA
                     LADRLAAALDHHLGERDLALMDTLERERRAVALQILAALLLAGLLAALAGGWLLRPLG
                     RMEQAIGELGENRLTRPIDIDGPADLRRLGERLDWLRLRLAELEADRGRVLRHVSHEL
                     KTPLASLREGVALLEDGVLGPLEPEQREVTAILAHSAHTLQARIEQLLRLNASQFDAR
                     SLHLQPTALLPLLRKIGEEQRLPARARRVTIGLAGDAPIVQADAGKLHSVFSNLLANA
                     IAFSPTGGHIRIELRRAGENVVIDCRDEGPGIAPDELERIFEPFYQGRRSPPSSAGGS
                     GIGLAIVREFIGAHRGRVRALPSELGAHFRVELPHA"
     gene            3979..4548
                     /locus_tag="PKV97_11315"
     CDS             3979..4548
                     /locus_tag="PKV97_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNB06510.1"
                     /translation="MFNKPNLFHGTGEVSAQRNAQRMAGTNVAAVPVTPPAETGGSAA
                     AREPQAVERADAAAAAGAVGESRLIVGPEVKLKGAEILDCDTLVVEGRVEATMDSRVL
                     RIAEKGAFKGTVGIDIAEIHGHFEGELTARKQLLIHAGGRVSGKIRYGKILIEEGGEL
                     SGDVQSLGAAGKELRAIDDKPRALAAVAG"
     gene            complement(4668..>5267)
                     /locus_tag="PKV97_11320"
     CDS             complement(4668..>5267)
                     /locus_tag="PKV97_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNB06511.1"
                     /translation="GAQAGAQQYEPMAASVRAALHAAVSDAVAPTLPIPDAGERAQWL
                     AEMSRRLARRIPDEAYRRELLTSIHYEATRAGLDPQMVLGLIQVESAFRKYAISSAGA
                     RGYMQVMPFWIKVIGREDDNLFHMRTNLRYGCTILRHYLDIEKGNLFRALGRYNGSLG
                     KPDYPNLVRAAWERHWTWTPQQLASAGASQPQSGATTAR"
BASE COUNT          673 a         1698 c         2147 g          749 t
ORIGIN      
        1 gatcaggtct gcgcgggctt gtccttgcgc tcgcccggag tagcggtggc gggcttgtca
       61 ccgctgcagg cggagacggt gtcgaacggt ccggcttcga gcaggcgggc ggcctgggcc
      121 ggggtggcga tcgagaaggc gatcgcggtg gtggtcagca tggcggcgat ggtctggcgt
      181 ttgatcatgt cgggctccgt ctgggtcttg ttcattgttc gacatcgggg ccggcgccgc
      241 ggaagagctc gggcgtcagg tcgtgccgct gcagcaggcg gtagaactcg gtgcgattgc
      301 gctccgccag ccgtgcagcg tccgaaacat tacccgcagt cagtttcagc agctgtatca
      361 gatagtttcg ctcgaagcgg cgcttcgcct cggcgtagcc gagggcctcg accggctgca
      421 cgcgcagggc gcgttcgacc agggcgcgcg ggatcagcgc cgtggtggcg agcgcgcacg
      481 cctgctcgac cacgttgtgg agctggcgca cgttgcccgg ccacggcgcg gtggcgagtg
      541 cctccagcgc ttcgggcgcg aagccggtga ggcgcctgcc gtacttgtgc gcgaggctgc
      601 gcaggaagtg cccggcgagc agcgggatgt cctcgcggcg ctcggccagg gtgggcagct
      661 gcaggctcac cacgtcgatg cggtagtaga ggtccgcgcg gaactggccc gcggccagcg
      721 ccgcgtcgag gtcgcggtgg gtggcggaga cgatgcgcag gtcgaccgcc tgcgcgcgcg
      781 tcgcacccac cggacgcacg gcgcgctcct gcagcacgcg cagcagcttg acctgcagcg
      841 cgggcggcat gtcgccgatc tcgtcgagga agagtgtgcc gccgtgggcg gcctgcacca
      901 ggccctgatg ggcgctggtg gcgccggtga aggcgccgcg cacgtggccg aagagctcgg
      961 attcgagcag cggctcggga atggcgccgc agttgatcgc gacgaagggc gcagcggcgc
     1021 gcgggctggc gaggtggatc gcgcgtgcga gcagctcctt gccggtgccg ctgtcaccgc
     1081 ggatcagcac gctggcgtcc gcggcggcga ccagacgggc ctcgtcgagg actgcctgca
     1141 tgcggctgct gcggctgagg atcgctgcgc gccaggccgt gtcgccggcg ctggcggggg
     1201 cttcggcgcg gagaccgctg cgggcgtggg tggggccggc gcgttgcagg gcctggcgga
     1261 tgccctgcag cagggcctgg ctgtcgaagg gcttggtgag ataaccggcg accccgagcg
     1321 cggtggcctc gaccgcgtcg gggatggtgc cgtgcgcggt gagcaggatg accggcagcg
     1381 ccggatgctg gcggcggatg tcctcgaaca gggcgagtcc gtcgccgtcg ggcaggcgca
     1441 cgtcgctcac cacgaggtcg aagcgctcca cgccgagacg gctgcgtgcg gccgcgacgc
     1501 tgtccgcggt ggcgacgtcg tagccggtgg cctgcaggcg catcgcgagc aggcgcagca
     1561 ggtcggcgtc gtcgtcgacc gccagcaggc gcgccggtgc cgccgccgcg gtcgcgtgca
     1621 tctccgtcga cgtgctcatt cgggcagccc ggtgcggggc cgcaccggca ggccgggcga
     1681 gttcagctcg cgttcgatct cggcgagcgc gtcgagctgg cgctgcagcg cggcgttctg
     1741 ccggcgcagc gcgtcgagtt ccagcgcctg cgcgtcgctg gcgcgggcac gctcgtcgag
     1801 ctggcggcgc agatgctcgg cgagtgcggc gacttgctgg cgcacgcgga gctgttcgag
     1861 cagggcgcgt gcgtagggat gcagcgcgcg cgcctgtgcg cgatcgtcgg cgagcaggct
     1921 ttcgagcgcg cgccgcgccg tggcgagccg ctcctgcccg ggctgggcgg cctgcaccgc
     1981 ggtgtgcagg cgctgctgcg cggcttcgtg ggcaagtgcc gcggcgctcg tgtcgtcctc
     2041 ggccgcctgc cgggccggga ggtcggcgca ccccgcgagc agggcgaggg tggcggggag
     2101 cacggcgcgg gcgatccgga cgcacgcgcg gcgtgctgcg cgggagactg cgcggacgac
     2161 gtggaggcgg gcgtcaggca tggggcaact cgacgcggaa atgggcgcca agctcgctcg
     2221 gcagcgcgcg cacccggccg cggtgggcgc cgatgaactc gcgcacgatc gccagcccga
     2281 tgccgctgcc gcctgcgctt gagggagggc tgcgacgacc ctggtagaag ggctcgaaga
     2341 tgcgttcgag ctcgtcgggc gcgatccccg ggccttcgtc gcggcagtcg atcaccacgt
     2401 tctcgccggc gcggcggagc tcgatgcgga tgtggccacc ggtggggctg aaggcgatcg
     2461 cgttggcgag caggttggag aacacgctgt gcagcttgcc ggcgtcggct tgcacgatgg
     2521 gagcgtcgcc cgcgagtccg atcgtcaccc ggcgtgcgcg cgccggcagg cgctgctcct
     2581 cgccgatctt gcgcagcagg ggcaggaggg cggtgggctg caggtgcagc gagcgcgcgt
     2641 cgaactggct cgcgttgagc cgcagcagct gttcgatgcg cgcctgcagg gtgtgcgcgc
     2701 tgtgcgcgag gatggcggtc acctcgcgct gctcgggttc gagcgggccg agcacgccgt
     2761 cctcgagcag ggcgacccct tcgcgcagcg aggcgagcgg ggtcttgagc tcgtgcgaga
     2821 cgtggcgcag cacgcggccg cggtcggcct cgagttcggc gaggcgcagg cgcagccagt
     2881 cgagccgctc gcccagcctg cgcaggtcgg cagggccgtc gatgtcgatc gggcgggtga
     2941 ggcggttctc gccgagctcg ccgatcgcct gttccatgcg cccgagcggg cgcagcagcc
     3001 acccgcccgc gagtgccgcg agcaggccgg cgaggagcag ggcggcgagg atctgcaggg
     3061 cgacggcacg gcgttcgcgc tcgagcgtgt ccatgagggc gagatcgcgc tcgccgagat
     3121 ggtggtccag cgccgcggcg agccggtcgg cgagcgcgcc gaggtggcgg aagtcgtccg
     3181 ccgtggcgag gccgtcgccg cgcccggccg cagtggcggg catgggctgt tcgcgcacgc
     3241 cgtcggtcgg cccgggtatg gcgaggctcg ccagccatgc ggcgatgcgg tccgcgcagg
     3301 cgcgccaggc cgcgcctgcg ctggcgagtg cggggccggc cgattccagc agcgcgagcg
     3361 cgggcagtgc ctcggcgagg gtggcctcga agcgccgggc gagtgccggc tcgcccagca
     3421 cctcgtactg gcgcgcgctg cgttcgaggt tcacgctgcg ttcgcggagc tgacgcgccg
     3481 cggcgctgag ggcgagcgcg cgctgcttgt cctcctggat cgcgcgcacg accgtttcca
     3541 tgccgcgcca gccgcccgcg acggcggcgg ccaggctggc ggtgatcagc aggaagctcg
     3601 cgagcaggat gctgcgaaag gagaggtgat gcatgcaggg agggggttga gcatcgcagc
     3661 ggagtgctgc gccggggtcg ccgagtctag gacggcgcgc gcggtcgcag cgtcgcgcaa
     3721 gtgcaagacg aagtaagcgg cgggtatcgg tgtcgccgcc gagcgacagc ggggaggcct
     3781 tgttccccgg aggcttgcgc ccgatctcgg gatgcggtgt cggggacggg cgacggtttc
     3841 cccgcgcgtg cgcccacgta ccgcccgggt cggtgcgcca agtgcccgca ggcaaaagga
     3901 aaaggtcctt tggcatggcg gttgctgaag gggctggaga gcgcagcgcc gtcgctgcgc
     3961 cggactccag gagagaacat gttcaacaag cccaatctgt tccacggcac cggcgaggtg
     4021 tccgcccagc gcaacgccca acgcatggca ggcacgaacg ttgccgcggt gcccgtgacg
     4081 ccgccggccg aaaccggcgg ttcggccgca gcgcgcgagc cgcaggcggt cgagcgcgcg
     4141 gatgcggcgg cggctgcggg ggccgtgggt gagagccgtc tgatcgtcgg tcccgaggtg
     4201 aagctcaagg gcgccgagat cctcgactgc gataccctgg tcgtggaggg gcgggtcgag
     4261 gcgacgatgg acagccgcgt gttgcgcatc gccgaaaagg gcgccttcaa gggcacggtc
     4321 ggcatcgaca tcgccgagat ccacggccat ttcgagggtg agctcaccgc ccgcaaacag
     4381 ctgctgatcc acgccggcgg gcgggtgagc ggcaagatcc gctacggcaa gatcctcatc
     4441 gaggaggggg gcgagctgtc tggcgacgtg cagtcgctgg gcgccgcggg caaggagctg
     4501 cgtgcgatcg acgacaagcc gagggcgctc gccgcggtgg cgggctgagc gggtcggcag
     4561 ggggcaggtc ccggcatggc agcggacgtg tccgcgcatg gcgcgagccg tgcggtgctg
     4621 cgtggagggg cggtagtgcc cctcgtgagc gcgatgccct ggtggggtca gcgcgccgtc
     4681 gtggcgccgc tttgcggctg ggaagcgccc gcggaggcaa gctgctgcgg cgtccaggtc
     4741 cagtgccttt cccaggcggc gcggaccagg ttggggtagt ccggcttgcc caggctgccg
     4801 ttgtagcgtc ccagcgcacg gaagaggttg cctttctcga tgtcgaggta gtggcgcagg
     4861 atggtgcagc cgtagcgcag gttggtgcgc atgtggaaga ggttgtcgtc ctcgcggccg
     4921 atgaccttga tccagaacgg catcacctgc atgtagccgc gcgcccccgc cgaggagatc
     4981 gcgtacttgc ggaaggcgct ctcgacctgg atcagcccga gcaccatctg cgggtcgagt
     5041 ccggcgcggg tggcctcgta gtggatgctg gtgaggagct ccctgcgata ggcctcgtcc
     5101 gggatgcgcc tggccagccg gcgcgacatc tcggcgagcc actgtgcacg ttcaccggcg
     5161 tcggggatcg gcagcgtcgg tgccacggcg tcgctgaccg cggcgtgcag cgcggcgcgc
     5221 acgctcgccg ccatgggctc gtactgctgc gcgccggcct gcgcgcc
//
LOCUS       DAOQNN010000219         5253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9445_length_5253_cov_8.650058, whole genome shotgun sequence.
ACCESSION   DAOQNN010000219 DAOQNN010000000
VERSION     DAOQNN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9445_length_5253_cov_8.650058"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="PKV97_11325"
     CDS             <1..1514
                     /locus_tag="PKV97_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB06512.1"
                     /translation="LAIVAVLGLLFVRMPTSFLPEEDQGVMFNQVMLPAGATQERTLE
                     VLKKVEHHFLENEKDTVRAIFTVAGFSFGGSGQHMGIGFVNLKHWDERKAPGKDVKSV
                     AGRAMGAFAQIREAMVFAFVPPAVIELGTSGGFTVQMQDRSGLGHGALLAARNQFLGM
                     AAQDKRLVGVRPNGQEDMPELKLDIDHAKAGALGLSVADINDTLSTAWGGSYINDFLD
                     RGRIKKVYLQGDAGSRMTPEDLDKWYVRNKAGDMVPFSAFATAHWTYGSPRLERFNGQ
                     PSMELLGQAAPGLSSGEAMKAVEEIMARLPPGIGYDWSGTSYEERRSGSQAPALYALS
                     LLVVFLCLAALYESWSVPFAVMLVVPLGVLGALLAASGRGLSNDVYFQVGLLATIGLS
                     SKNAILIVEFAKAQMEEGKDLISATLTAVRMRLRPIIMTSLAFGFGVLPLAIATGAGA
                     GSQNAIGTGVLGGTIGATLLGLFFVPLFYVVIKRIFPDKPKGEAVAPIAQEGR"
     gene            1516..2994
                     /gene="adeC"
                     /locus_tag="PKV97_11330"
     CDS             1516..2994
                     /gene="adeC"
                     /locus_tag="PKV97_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AdeC/AdeK/OprM family multidrug efflux complex
                     outer membrane factor"
                     /protein_id="HNB06513.1"
                     /translation="MKTHAKALLVPDSVPVSSVRRARAATTLRSLVVALAAATLGACS
                     MIPVFERPAAPVPATFPQATPTAAPVVAPLADTIAWRDYFADQRLREVIALALENNRD
                     LRVAALNIERARAQYGIQQADLFPSIAVSGGQSAQRVPGDLSMTGDSNVSRQYSANLG
                     FASYELDFFGRVRSLEEQALQNYLATEEARRSAQISLVAEVANAWLRLAADRERLALA
                     HNTRQTRQASFDLVRRSLELGAVSALDVHQAETLLQSARADAARFETVVAQDRNVLAQ
                     LVGGGVPDTLLPDGLEQAVATVAELPAGVPSEVLTRRPDIVQAERALLAANASIGAAR
                     AAFFPRITLTATAGTASSTLDGLFDGGSGAWSFVPQLRLPIFEAGRLQASLDLAEIQR
                     DINVAQYEKAIQSAFREVADALAERATVAERIDARRKQVAATQNSFKLSDARYRGGVD
                     SYLSLLDAQRSLYAAELELIGVRLIEATNRVELYKALGGGWQ"
     assembly_gap    3041..3140
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3196..4329
                     /locus_tag="PKV97_11335"
     CDS             3196..4329
                     /locus_tag="PKV97_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNB06514.1"
                     /translation="MNIPAQSADVDRRIPVTLLTGFLGAGKTTLLNHLLRQPQMEGSA
                     VLINEFGAVGVDHHLVEKVDESLVVLDSGCICCSVQGDLVRALKSLFMRALRRELKGL
                     RRVLIETTGLADPAPVIHTLMAEPFLSERYRLDGVVTAVDLTHAREQLTHHNEAVRQV
                     AMADRVLLTKGDLAADAMRAEVGALVAALNPGARQIEVAGGEVGPEAVFGCGLYDPAG
                     KTPDVAAWLGEEAVRAARRAPVAPLWRRPGRSGAGVHSLTAAEQENSRHTPGVSAFVL
                     VFDEPLDWFGFSDGLALLLQVYGGRILRIKGLLNVAGDPLPRVLQCVQHSVYPASSLP
                     AWPDSAPYDDRRSRLVFIVRELAEDEVISILGSFTGQLPRRAD"
     gene            complement(4409..4894)
                     /locus_tag="PKV97_11340"
     CDS             complement(4409..4894)
                     /locus_tag="PKV97_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06515.1"
                     /translation="MQHTSTVRLALLALVLAGSLGATAAHAEALACPTLSTATQVQPC
                     PTEDELKYTYTGYCSDNARLYGRDVLTCASFENYKEVKNVALWEAEGGKFDGYLSCNV
                     DAASIKASKAQKLSVERVKTLTRVICDYENDHRLVHRTKSECVVEAADCSGAECKARC
                     D"
     gene            complement(4960..>5253)
                     /locus_tag="PKV97_11345"
     CDS             complement(4960..>5253)
                     /locus_tag="PKV97_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNB06516.1"
                     /translation="AVEESLMRVSNYPEFATHKQNHEDLIAQVKALQDKLDSGQATIT
                     FELLHFLKVWLTRHINEADKRFGAYFVEAGGASKWSDHVEQAMKKRKWWWKFW"
BASE COUNT          722 a         1805 c         1794 g          832 t
ORIGIN      
        1 acctcgccat cgtcgcggtg ctcggcctgc tcttcgtgcg catgccgacc tccttcctgc
       61 ccgaggaaga ccagggcgtg atgttcaacc aggtcatgct gcccgccggc gccacccagg
      121 agcgcacgct cgaggtgctg aagaaggtcg agcaccactt cctcgagaac gaaaaggaca
      181 ccgtgcgcgc gatcttcacc gtcgccggct tttccttcgg cggcagcggc cagcacatgg
      241 gcatcggctt cgtgaacctc aagcactggg acgagcgcaa ggcgccgggc aaggacgtga
      301 agtcggtggc cgggcgcgcg atgggcgcgt tcgcgcagat ccgcgaggcg atggtgttcg
      361 ccttcgtgcc gccggcggtg atcgagctcg gcacctcggg cggcttcacc gtgcagatgc
      421 aggaccgctc cggcctcggc cacggcgccc tgctcgccgc gcgcaaccag ttcctcggca
      481 tggcggcgca ggacaagcgc ctggtcggtg tgcgccccaa cggccaggag gacatgcccg
      541 agctcaagct cgacatcgac cacgccaagg cgggtgcgct cggcctctcg gtcgccgaca
      601 tcaacgacac cttgtcgacc gcctggggcg gcagctacat caacgacttc ctcgaccgcg
      661 gccgcatcaa gaaggtgtac ctgcagggcg acgcgggctc gcgcatgacg cccgaggacc
      721 tcgacaagtg gtacgtgcgc aacaaggcgg gcgacatggt gccgttctcg gccttcgcca
      781 ccgcgcactg gacctacggc tcgccgcgcc tcgaacgctt caacggccag ccgtcgatgg
      841 aactgctcgg ccaggccgcg cccgggctgt cgtccggcga ggcgatgaag gcggtcgagg
      901 agatcatggc caggcttccg ccggggatcg gctacgactg gtcgggcacc tcctacgagg
      961 aacgccgttc gggctcgcag gcgcctgccc tctatgcgct gtcgctgctg gtggtgttcc
     1021 tgtgcctggc ggcgctgtac gagagctggt cggtgccctt cgcggtgatg ctggtggtgc
     1081 cgctcggcgt gctcggcgcg ctgctcgccg ccagcgggcg cgggctgtcg aacgacgtgt
     1141 atttccaggt cggcctgctc gccaccatcg gcctgtcgtc gaagaacgcg atcctgatcg
     1201 tggagttcgc caaggcgcag atggaggagg gcaaggatct gatctccgcc acgctgaccg
     1261 cggtgcgcat gcgtctgcgc ccgatcatca tgacctcgct cgccttcggc ttcggcgtgc
     1321 tgccgctggc gatcgccacc ggcgccggcg ccggcagcca gaacgcgatc ggcaccggcg
     1381 tgctcggcgg caccatcggc gcgacgctgc tcggcctgtt cttcgtgccg ctgttctacg
     1441 tcgtgatcaa gcgcattttc ccggacaagc ccaagggtga ggccgtggcg ccgatcgccc
     1501 aggagggacg ttgagatgaa gacccacgca aaagccctgc tcgtgccgga ttcggtgccg
     1561 gtgtcgagcg tccgtcgcgc gcgtgcagcc acgacgctgc gcagcctggt agtggcgctc
     1621 gccgccgcca cgctcggcgc ctgctcgatg atcccggtgt tcgagcgccc cgcggcgccg
     1681 gtgccggcga ccttcccgca ggcgacgccg accgcggccc cggtggtggc gcccctggcc
     1741 gacaccatcg cctggcgcga ctacttcgcc gatcagcgcc tgcgcgaggt gatcgcgctc
     1801 gcactcgaga acaaccgcga cctgcgcgtc gccgcgctca acatcgagcg cgcgcgcgcg
     1861 cagtacggca tccagcaggc ggacctgttc ccgtcgatcg cggtcagcgg cgggcagagt
     1921 gcgcagcgcg tgccgggcga cctgagcatg acgggtgact ccaacgtcag ccgccagtac
     1981 tcggccaacc tcggcttcgc cagctacgag ctcgatttct tcggccgcgt ccgcagcctc
     2041 gaggagcagg cgctgcagaa ctacctcgcg accgaggagg ctcgccgcag cgcgcagatc
     2101 agcctggtgg ccgaagtcgc caatgcctgg cttcgcctgg ctgcagaccg cgaacgcctg
     2161 gcgctggcgc acaacacccg gcagacccgc caggcgtcgt tcgatctcgt gcggcgcagc
     2221 ttggagctgg gggcagtgtc ggcgctcgac gtgcatcagg ccgagaccct gctgcagagc
     2281 gcgcgtgccg atgcggcacg tttcgagacc gtggttgcgc aggaccgcaa cgtgctcgcg
     2341 caactggtcg gcggcggtgt cccggacacg ctgttgccgg acgggctcga gcaggcggtt
     2401 gcgacggtgg cggagctccc cgccggggtg ccctccgagg tgctcacgcg ccgtcccgac
     2461 atcgtgcagg ccgaacgtgc cctgctcgcg gccaatgcca gcatcggcgc tgcgcgtgca
     2521 gccttcttcc cgcgcatcac gctgaccgcc accgccggca ccgcgagcag cacgctcgat
     2581 ggcctgttcg acggcggctc gggggcgtgg agtttcgtgc cgcagttgcg cctgccgatc
     2641 ttcgaggccg gccgcctcca ggccagcctg gatctcgccg agatccagcg cgacatcaac
     2701 gtcgcccagt acgagaaggc gatccagtcg gcgttcaggg aagtggccga cgccctcgcc
     2761 gagcgcgcga ccgtcgccga gcgcatcgac gcccggcgca agcaggtggc ggcgacgcag
     2821 aacagcttca agctgtccga cgcccgctac cgcggtggtg tcgacagcta cctgagcctg
     2881 ctcgacgccc agcgcagcct ctacgcagcc gaactggagc tgatcggcgt gcgcctcatc
     2941 gaggcgacca accgcgtcga actctacaag gcgctcggcg gcggctggca gtgaccgccc
     3001 cttcccaccc accgcgatcc ggccgcccgt aggcgcgacg nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn ctcgtgctcg cggcaggctt ctgatcgtcg ggtgcgccgg
     3181 ctacactgcg gcgccatgaa cattcccgcg caatccgccg acgtcgaccg ccgcattccg
     3241 gtgacgctgc tcaccggctt cctcggtgcc ggcaagacca cgctgctcaa ccacctgctg
     3301 cgccagccgc agatggaggg cagcgcggtg ctgatcaacg agttcggcgc cgtcggggtc
     3361 gaccaccacc tggtggagaa ggtggacgag agcctggtcg tgctcgactc gggctgcatc
     3421 tgctgcagcg tgcagggcga cctggtgcgc gcgctcaaga gcctcttcat gcgtgcgctg
     3481 cggcgcgagc tgaagggcct gcgccgggtg ctgatcgaga ccaccgggct cgccgacccc
     3541 gcgccggtga tccacaccct gatggccgag cccttcctgt ccgagcgcta ccggctcgac
     3601 ggcgtggtga ccgcggtcga cctcacccat gcgcgcgaac agctcaccca ccacaacgag
     3661 gcggtgcgcc aggtcgcgat ggccgaccgg gtgctgctga ccaagggcga tctggccgcc
     3721 gacgcgatgc gcgccgaggt gggtgcgctg gtggccgcgc tcaaccccgg cgcgcgccag
     3781 atcgaggtgg cgggcgggga ggtggggccg gaggcggtgt tcggctgcgg tctgtacgac
     3841 cccgctggca agacccccga cgtggccgcc tggctgggcg aggaggcggt gcgcgccgcg
     3901 cgtcgtgcgc ccgttgcgcc gctttggcgc aggccgggcc ggagcggggc cggcgttcac
     3961 tctctcacgg ccgcggagca ggagaactcg cgccacaccc ccggcgtgag cgccttcgtg
     4021 ctcgtcttcg acgagccgct cgactggttc ggcttctccg acgggctcgc cttgctgctg
     4081 caggtctacg gcggacgcat cctgcgcatc aagggcctgc tcaacgtcgc cggcgacccg
     4141 ctgccgcggg tactgcagtg cgtgcagcac agcgtctatc cggcgagcag cctgccggcc
     4201 tggccggatt ctgcgcccta cgacgaccgc cgcagccggc tggtgttcat cgtgcgcgag
     4261 ctcgccgagg acgaggtgat ctccatcctg ggcagcttca ccgggcagct gccgcgccgc
     4321 gcggactgaa gggacgaatc cgccgccgcc cgcgtccgag ctccggtccc gccgcctgcg
     4381 tcgcggtccc gcggccggct cggcttactc agtcgcagcg cgccttgcac tcggcgccgc
     4441 tgcagtccgc ggcctccacc acgcactccg acttggtgcg atgcacgagg cggtggtcgt
     4501 tctcgtagtc gcagatcacg cgggtgagcg tcttgacgcg ctcgacgctc agcttctgtg
     4561 ccttcgaggc cttgatcgag gccgcgtcga cgttgcagga caggtagccg tcgaacttgc
     4621 cgccctcggc ctcccacagt gcgacgttct tcacttcctt gtagttctcg aagctcgcgc
     4681 aggtgagcac gtcgcggccg tacaggcggg cgttgtcgct gcagtagccg gtgtaggtgt
     4741 acttgagttc gtcctcggtc gggcagggct ggacctgggt cgccgtggac agcgtggggc
     4801 aggcgagcgc ttcggcgtgt gcggcggtgg cgccgaggct gccggcgagg acgagggcga
     4861 ggagggcgag tcggaccgtg gaagtgtgct gcatcgtcgt gtttccggaa gggtgagtcg
     4921 ggttgcctgt ggaggagtct gaggcctggc ctgccgttct taccagaact tccaccacca
     4981 cttgcgcttc ttcatcgcct gctcgacgtg atcggaccac ttgctggctc cgccggcctc
     5041 gacgaaatac gcgccgaagc gcttgtccgc ctcgttgatg tggcgcgtga gccatacctt
     5101 gaggaagtgc aggagttcga aggtgatggt cgcctggccg gagtcgagct tgtcctgcag
     5161 ggccttcacc tgggcgatca ggtcctcgtg gttctgcttg tgggtggcga attcggggta
     5221 attcgacacc cgcatcaggc tctcctcgac cgc
//
LOCUS       DAOQNN010000220         5245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9465_length_5245_cov_12.322351, whole genome shotgun sequence.
ACCESSION   DAOQNN010000220 DAOQNN010000000
VERSION     DAOQNN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9465_length_5245_cov_12.322351"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="PKV97_11350"
     CDS             <1..1250
                     /locus_tag="PKV97_11350"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNB06517.1"
                     /translation="AIVEVGGTVGDIESLPFLEAIRQMGFEEGRHGTCFIHLTLLPYI
                     PTAGELKTKPTQHSVKELREIGIQPDILLCRADRPVPEDERRKIALFCNVMPEAVIEC
                     LDADSIYKIPGQLHDQMLDQIVCHKLDILARAADLSVWDRLIHALENPKQTVDIGFVG
                     KYVDLTESYKSLIESLNHAGMHTESKVRIHYIDSEDIERDGCDVLESMDAILVPGGFG
                     KRGTEGKIAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMEGAHSTEFERDTRFPV
                     IGLITEWKDRTGKIEKRSEESDLGGTMRLGGQLCLLKEGTLARSIYGAPEVMERHRHR
                     YEVNNTLLARLEDKGLVVSGRAPVTDLCEMVELAADVHPWFVGCQFHPEFTSNPRKGH
                     PLFTAYVKAAIAHKQAAAPRSEA"
     gene            1247..2086
                     /gene="kdsA"
                     /locus_tag="PKV97_11355"
     CDS             1247..2086
                     /gene="kdsA"
                     /locus_tag="PKV97_11355"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNB06518.1"
                     /translation="MNLCGFEVGLDKPVFLISGPCVAESEQMCLDIAGQMKEICAELG
                     IPYIFKASYDKANRSSGKSFRGHGMDAGLKMLEAVKKQLGLPVLTDVHTIEEIPTVAA
                     VVDVLQTPAFLCRQTDFIHAVAASGKPVNIKKGQFLAPGDMKNVADKAREANGGADTI
                     MVCERGASFGYNNLVSDMRALAIMRDTGCPVVFDATHSVQLPGGQGTASGGQREFVPV
                     LARAAVAVGVAGLFMETHPDPAKALSDGPNAWPLPKMKALLTVLKDIDALVKRQGFLE
                     TTL"
     gene            2242..3525
                     /gene="eno"
                     /locus_tag="PKV97_11360"
     CDS             2242..3525
                     /gene="eno"
                     /locus_tag="PKV97_11360"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNB06519.1"
                     /translation="MSAIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTG
                     SREAIELRDGDKSRFLGKGVLRAVENVNTEIAEAIIGLDAEEQAFIDRTLIELDGTEN
                     KSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSSPMVMPVPMMNVINGGEHANNSL
                     DIQECMIMPVSMTSFREALRCGAEIFHHLKKIVDAKGYPTTVGDEGGFAPNVSGTEEA
                     LNLIQEAIVAAGYVPGQDVLLALDCAASEFYKDGKYELKGEGLSLTAAGFTDYLETLC
                     DKFPIVSIEDAMAEGDWDGWKILTERLGKRVQLVGDDLFVTNTRILAQGIEQGVANSI
                     LIKINQIGTLTETFAAVEMAKLAGYTAVISHRSGETDDSTIADIAVGLRAMQIKTGSL
                     SRSDRISKYNQLLRIEEDLGDTASYPGKRAFYNLR"
     gene            3625..3897
                     /gene="ftsB"
                     /locus_tag="PKV97_11365"
     CDS             3625..3897
                     /gene="ftsB"
                     /locus_tag="PKV97_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HNB06520.1"
                     /translation="MRWPILILIALVVLLQYPLWLGKGGWLRVWEVDRQLHAQREENL
                     RLEQRNAALAAEVNDLKSGNEAIEERARFELGLTRPGEIYVQIPQR"
     gene            complement(3938..4522)
                     /locus_tag="PKV97_11370"
     CDS             complement(3938..4522)
                     /locus_tag="PKV97_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNB06521.1"
                     /translation="MSPEPLPRAFLSAPLHAGIGKRRLALLAGVATVAALLAGCGTTP
                     VAGPGSPPRTVAPSGHYFSLEGEHQTQEMVLFALGLLDTGYRFGGRNPDAGLDCSGMV
                     AYIVENISGRKLPHNAAQIADRTRPIEVHELRPGDLVFFNTLERRHSHMGIYIGDGRF
                     VHAPNSRGRVRVERLASRYFAQRIDGARTLLASN"
     gene            complement(4661..>5245)
                     /locus_tag="PKV97_11375"
     CDS             complement(4661..>5245)
                     /locus_tag="PKV97_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305918.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNB06522.1"
                     /translation="SGPAANDLAVAVMYAERSGASAAAVAREVNRDIAAALELARAQG
                     AVKVQSGNVSTWPAYGKDGQGRITAWRMRSELRLESTDTAAMSELVGKLQESLALAQL
                     SMEPAPETRRKAVSDVTVDALRAFEARAKLIADTLGRRYRIAHLSVGEHGLQPPPMPR
                     MRAAAMAAEAAPAPLEGGESRVSVQVGGRIELLD"
BASE COUNT          882 a         1774 c         1763 g          826 t
ORIGIN      
        1 tggcgatcgt cgaggtcggc ggcacggtgg gcgacatcga gtcgctgccc ttcctggagg
       61 ccatccgcca gatgggcttc gaagagggcc gccacggcac ctgcttcatc cacctcacgc
      121 tgctgcccta cattccgacc gccggtgaac tgaagaccaa gccgacccag cactcggtga
      181 aggagctgcg cgagatcggc atccagcccg acatcctgct gtgccgcgcc gaccgtccgg
      241 tgccggaaga cgagcgccgc aagatcgcgc tgttctgcaa cgtgatgccc gaggcggtca
      301 tcgagtgcct ggacgccgac tcgatctaca agatccccgg tcagctgcat gaccagatgc
      361 tcgaccagat cgtctgccac aagctcgaca tcctcgcgcg cgcggccgac ctctcggtgt
      421 gggacaggct catccacgcg ctcgagaacc cgaagcagac cgtggatatc ggtttcgtcg
      481 gcaagtacgt ggaccttacc gagtcgtaca agtcgctgat cgagtcgctc aaccacgccg
      541 gcatgcatac cgagtcgaag gtcaggatcc actacatcga ctcggaggat atcgagcgcg
      601 acggttgcga tgtgctcgag tcgatggatg ccatcctggt gccgggcggc ttcggcaagc
      661 gcgggaccga gggcaagatc gcggccatcc gctatgcgcg cgagaacaag gtgccctacc
      721 tgggcatctg tctcggcatg cagctcgcgg tggtggagtt cgcccgcgac gtcgcgggca
      781 tggaaggcgc gcactcgacc gagttcgagc gcgacaccag gttcccggtg atcggcctca
      841 tcaccgagtg gaaggaccgc acgggcaaga tcgagaagcg ctccgaggag tccgacctcg
      901 gtggcaccat gcgcctcggc ggccagctct gcctgctcaa ggaaggcacg ctggcgcgtt
      961 cgatctacgg cgcccccgag gtcatggagc gtcaccgcca ccgctacgag gtgaacaaca
     1021 ccctgctcgc caggctcgaa gacaagggcc tggtggtgtc gggccgcgcg cccgtcaccg
     1081 acctgtgcga gatggtcgag ctggctgcgg acgtgcatcc gtggttcgtc ggctgtcagt
     1141 tccatcccga attcacctcc aacccgcgca agggtcatcc gctgttcacc gcttatgtga
     1201 aggctgcgat cgcgcacaag caggctgcgg ccccgaggag cgaagcatga atctctgcgg
     1261 tttcgaagtc ggcctcgaca agccggtgtt cctgatctcc ggcccctgcg tggccgaatc
     1321 cgagcagatg tgcctggaca tcgccgggca gatgaaggag atctgcgccg agctgggcat
     1381 cccttacatc ttcaaggctt cgtacgacaa ggccaaccgc agctcgggca agagcttccg
     1441 cggccatggc atggacgccg gcctgaagat gctcgaggcg gtcaagaagc agctcggcct
     1501 gccggtgctg accgacgtgc acacgatcga ggagatcccg accgtcgccg cggtggtcga
     1561 cgtgctgcag accccggcct tcctgtgccg ccagaccgat ttcatccacg cggtggcggc
     1621 cagcggcaag ccggtgaaca tcaagaaggg ccagttcctc gcccccggcg acatgaagaa
     1681 cgtcgccgac aaggcgcgcg aggccaatgg cggcgccgac accatcatgg tgtgcgagcg
     1741 cggcgcctcc ttcggctaca acaacctcgt ctccgacatg cgtgcgctgg cgatcatgcg
     1801 cgacaccggc tgcccggtgg tgttcgacgc tacccactcg gtgcagctgc cgggcgggca
     1861 gggaaccgcc tcgggtggcc agcgcgagtt cgtgccggtg ctggcgcgcg ccgcggtcgc
     1921 cgtgggcgtg gcgggccttt tcatggagac ccacccggac cccgccaagg cgctgtccga
     1981 cggccccaac gcctggccgc tgccgaagat gaaggcgctg ctgaccgtgc tcaaggacat
     2041 cgacgcgctg gtcaagcgcc agggtttcct cgagaccacg ctctgagacc ccatttttcg
     2101 ggaatgcggc taccggcgga ctgatgcgcg tcatccgtcg ccgctgctca caaggcggtc
     2161 atccggtgcg agcatgctcc ccgccggatc gcgttcccgc atgatcgatc cgaaacctcc
     2221 gtaactgcta caggactaga catgagtgca atcgttgatg tgatcgcccg cgagattctc
     2281 gattcccgcg gcaaccccac ggtcgaagcc gacgtgctgc tcgaatcggg cgtgatgggc
     2341 cgcgccgcgg tgccctcggg cgcctccacc ggctcgcgcg aggcgatcga gctgcgtgac
     2401 ggcgacaagt cgcgcttcct cggcaagggc gtgctgcgcg cggtcgagaa cgtgaacacc
     2461 gagatcgccg aggccatcat cgggctggac gccgaggagc aggccttcat cgaccgtacc
     2521 ctgatcgagc tcgacggcac cgagaacaag tcgcgcctgg gcgccaacgc catgctggcg
     2581 gtgtcgatgg cggtggccaa ggctgccgcg gaagaggccg gcctgccgct gtaccgctac
     2641 ttcggcggct ccagcccgat ggtgatgccg gtgccgatga tgaacgtgat caacggcggc
     2701 gagcacgcca acaacagcct cgacatccag gaatgcatga tcatgccggt gagcatgacc
     2761 agcttccgcg aggccctgcg ctgcggcgcc gagatcttcc accatctcaa gaagatcgtc
     2821 gacgccaagg gctaccccac caccgtcggc gacgagggcg gcttcgctcc caacgtgtcg
     2881 ggcaccgagg aagcgctcaa cctgatccag gaggccatcg tcgccgccgg ctacgtgcct
     2941 ggccaggacg tgctgctggc gctcgactgc gccgcctccg agttctacaa ggacggcaag
     3001 tacgagctga agggcgaagg cctgtcgctg accgccgcgg gcttcaccga ctacctcgag
     3061 accctgtgcg acaagttccc gatcgtctcg atcgaggacg ccatggccga gggcgactgg
     3121 gacggctgga agatcctcac cgagcgcctg ggcaagcgcg tgcagctggt gggcgacgac
     3181 ctcttcgtca ccaacaccag gatcctcgcg cagggcatcg agcagggcgt ggccaactcg
     3241 atcctcatca agatcaatca gatcggcacg ctcaccgaga ccttcgcggc agtcgagatg
     3301 gccaagctcg ccggctacac cgcggtgatt tcgcaccgct cgggcgagac cgacgattcg
     3361 accatcgccg acatcgcggt gggcctgcgc gccatgcaga tcaagaccgg ctcgctgagc
     3421 cgctcggacc gcatctcgaa gtacaaccag ctgctgcgca tcgaggaaga cctcggcgac
     3481 accgcttcct acccgggcaa gcgcgcgttc tacaacctgc gctgaagcac cctgctgcgc
     3541 tgatccgcag cttccggaag gccggcgggc gacccgccgg ccttcgtcgt gaatgcgcct
     3601 gaactcatct ccgtacccgt ctccatgcgc tggccgatcc tgatcctgat cgcactcgtc
     3661 gtgctgctgc agtacccgct ctggctgggg aaagggggct ggctgcgcgt ctgggaggtc
     3721 gatcgccagc tgcatgcgca gcgcgaggag aacctgcgcc tcgagcagcg caacgccgcg
     3781 ctcgcggcgg aggtgaacga tctcaagtcg ggcaacgagg cgatcgagga gcgcgcgcgt
     3841 ttcgaactcg ggctcacccg gccgggcgag atctacgtgc agattccgca gcgctgaacc
     3901 ctggccgcgg gtatcgccgc cgttccggcg acagtcctca gttgctcgcg agcagcgtgc
     3961 gcgcgccgtc gatgcgctgc gcgaaatagc ggctggcgag acgttcgacc cgcacccggc
     4021 cacggctgtt gggcgcgtgc acgaagcgcc cgtcgccgat gtagatcccc atgtgcgaat
     4081 gccggcgctc cagcgtgttg aagaacacca ggtcccccgg gcgcagttcg tgcacctcga
     4141 tcgggcgcgt gcgatcggcg atctgcgcgg cgttgtgcgg cagcttgcgg ccgctgatgt
     4201 tctccacgat gtaggccacc atgccgctgc aatcgaggcc cgcgtccgga ttgcgcccgc
     4261 cgaagcggta gccggtatcg agcaggccga gcgcgaagag caccatttcc tgcgtctggt
     4321 gctcaccctc gagagagaaa tagtgcccgc tcggcgccac cgtgcgcggt ggcgagccgg
     4381 ggccggcgac cggggtggtg ccgcagcccg ccagcagtgc ggccacggtg gccacgccgg
     4441 caagcagtgc caggcgccgc ttgccgatgc cggcgtgcag aggggcggaa aggaaggcgc
     4501 gagggagggg ctcgggactc atggcgcgat ccggtgcaaa aaggatttct gccagagtat
     4561 gtcaggaccc gcccaggccg gcaaggtctg ctttcagatc aggggtttgg agaggttttc
     4621 tcgaagaagg ctgaagtcgg gcccgcgtgg ggcgcggtcg tcagtcgagc agttcgatgc
     4681 gtcctccgac ctgcaccgac acccggctct cgccgccctc gagtggcgcc ggggcggcct
     4741 ccgcggccat cgccgccgcg cgcatgcgcg gcatcggcgg aggctgcagg ccgtgctcgc
     4801 cgaccgacag gtgggcgatg cggtagcgcc gaccgagggt gtcggcgatc agtttcgcgc
     4861 gcgcctcgaa ggcgcgcagg gcgtcgacgg tgacgtcgga gacggccttc ctgcgcgtct
     4921 cgggggctgg ttccatgctc agctgggcga gcgccaggct ctcctggagc ttgccgacga
     4981 gctccgacat cgccgccgtg tcggtgctct cgaggcggag ctcggaacgc atccgccagg
     5041 cggtgatacg gccctggccg tcctttccgt atgccggcca ggtcgagacg tttccgctct
     5101 gcaccttgac ggcaccctgc gcgcgcgcga gttcgagcgc cgcggcgatg tcgcggttca
     5161 cctcgcgtgc caccgcggcc gcgctcgcgc cgctgcgctc tgcatacatc acggcgaccg
     5221 cgaggtcgtt ggcggccggg ccgct
//
LOCUS       DAOQNN010000221         5223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9526_length_5223_cov_8.836494, whole genome shotgun sequence.
ACCESSION   DAOQNN010000221 DAOQNN010000000
VERSION     DAOQNN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9526_length_5223_cov_8.836494"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2167)
                     /locus_tag="PKV97_11380"
     CDS             complement(<1..2167)
                     /locus_tag="PKV97_11380"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNB06523.1"
                     /translation="MAEVGNPVRAKSMRDDRDDFINLGEIIAVLLEYKWLILAVTFFA
                     VFIGAVVAFVSTPIYRADGLVQVQDSKGPKGGLAALRDVEAVLGENSSVTAELEILRS
                     RMILGRVVERLRLDIRATPDYFPIFGRALARRYNGEQPAAPLLGLGSYAWGGERITVQ
                     TLEVPSYLVGLPLTLIAGDNGGFTLYDDQDQPLLDGSVGQPASSADGKTTLFVAELVA
                     RPGTHFDLARISQIQAIAALREDLEVRERARQSNVIEAAYSDADRAEAERLLNEVLNA
                     YVRQNVEYRSAEADATLAFLEKQLPELKAQLDTAEAAYNDYRQTRGSVDLTLETQSVL
                     SSIVKVDADVVELQQKRDELRQRFTPEHPQVKAIDSQLGRLRAVRGTLDKDVNRLPDT
                     QQTALRLRRDVEVATALYTNLLNSAQQLRVARAGTVGDVRVIDPAATAPLPVAPRKAL
                     ILLLSGVLGVLGSLGLVWAIRSLRVVVEDPQTIERELSLPVYATVPASKDEAVLSRAI
                     ARGKMDKGQLLAIANPDDDAMESLRSLRTTLHFALLGAEKGSVLITGPAPGVGKSFIS
                     KNLGAVLAQAGKRVMLVDGDLRKGHINKAFGIGRGVGVSDYIMGAASIEQIVKPTGID
                     NFALVTTGQIPPNPSELLMHPRFAALLAELEKQCDVLIIDAPPVLAVSDAAIIGRQVG
                     ATLLVARAGRHPVRELEQAIKRFDQAGVEVKGFVFNGFDL"
     assembly_gap    2255..2354
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2464..3759)
                     /locus_tag="PKV97_11385"
     CDS             complement(2464..3759)
                     /locus_tag="PKV97_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HNB06524.1"
                     /translation="MSRFTTDRIAHCGASNGAVRATDASGLDARAAGPATRADATRSG
                     GLVRTAVIAFAAAAIGGCTLIPGTSGSLMREESSVPLPVTEGTDTVPANVKLKPITAE
                     LIIEQHQAKESRFRSGKAGGQTATRASDPKKIPGFDYQDYKLGPGDIINVIVWDHPEI
                     TIPAGSYRSAEQSGTLVAEDGTIFFPFAGVVKVAGLTTREVRQVLAKRMASVIENVQL
                     DVRIVSFRSKRVYVVGEVAKPGLQPIDDIRMTLVEAINRAGNITEEADHGNVLLTRNG
                     QTWRVDLQALYEEGDVSQNVLLQPGDIINVPDRQLNKVFVLGEVRNPGSFVMNKRRTT
                     LAEALSDAGFVNQSTSDPAWVYVMRSDNGSAELFHLNARSPDALLLAERFPLLPRDVV
                     YVDVAAIARWNRVVSNILPTSQMLQLTSETRYPLFGGRQ"
     gene            complement(3756..3911)
                     /locus_tag="PKV97_11390"
     CDS             complement(3756..3911)
                     /locus_tag="PKV97_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06525.1"
                     /translation="MTPLEPAPRLDPGIIGKAPETHIVQANMPACRDAAAEARAGQAA
                     KLERGFR"
     gene            4738..4953
                     /locus_tag="PKV97_11395"
     CDS             4738..4953
                     /locus_tag="PKV97_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06526.1"
                     /translation="MCTFDAPIARCEAMNQMVLLDETRRECAFEHGCPPGQKCPLNGC
                     FARQSGLYDAHPEIGAHGQPRVAHHAH"
BASE COUNT          750 a         1636 c         1844 g          893 t
ORIGIN      
        1 tgaggtcgaa gccgttgaac acgaatccct tgacctcgac cccggcctgg tcgaagcgct
       61 tgatggcctg ctcgagctcg cgcaccgggt ggcggccggc gcgggcgacc aggagggtgg
      121 cgccgacctg gcggccgatg atggcggcgt cggacacggc gagcaccggg ggcgcgtcga
      181 tgatgagcac gtcgcactgc ttttcgagct cggcgagcag cgcggcgaag cgcgggtgca
      241 tgaggagctc ggacgggttg ggcgggatct ggccggtggt gacgagggcg aagttgtcga
      301 tgccggtggg cttgacgatc tgctcgatgc tggcggcgcc catgatgtag tcggacacgc
      361 ccacaccgcg gccgatgccg aaggccttgt tgatgtggcc cttgcgcagg tcgccgtcga
      421 ccagcatgac gcgcttgccg gcctgggcga gcacggcgcc gaggttcttg ctgatgaagc
      481 tcttgccgac gccgggcgcc gggccggtga tgagcaccga gcccttctcg gcgccgagca
      541 gcgcgaagtg cagcgtggtg cgcaggctgc gcaggctctc catggcgtcg tcgtcggggt
      601 tggcgatggc gagcagctgg cccttgtcca ttttgccgcg ggcgatggcg cgcgagagca
      661 cggcttcgtc cttgctggcg ggcacggtgg cgtacaccgg cagcgagagt tcgcgctcga
      721 tggtctgcgg gtcttcgacg accacgcgca ggctgcggat ggcccacacc aggccgagcg
      781 agccgagcac gccgagcacg cccgagagca gcaggatgag cgccttgcgc ggggcgaccg
      841 gcagcggcgc ggtggcggcg gggtcgatca cgcgcacgtc gccgacggtg ccggcgcgcg
      901 ccacgcgcag ctgctgggcg ctgttgagca ggttggtgta cagcgcggtg gcgacctcga
      961 cgtcgcggcg caggcgcagc gcggtctgct gggtgtcggg gaggcggttg acgtccttgt
     1021 cgagggtgcc gcgcacggcg cgcaggcggc cgagctgcga gtcgatggcc ttgacctggg
     1081 ggtgctcggg ggtgaagcgc tggcgcagct cgtcgcgctt ttgctggagc tcgacgacgt
     1141 cggcgtccac cttgacgatg gagctgagca ccgactgggt ctcgagggtg aggtcgaccg
     1201 agccgcgggt ctggcggtag tcgttgtagg cggcttcggc ggtgtcgagc tgggccttga
     1261 gctcggggag ctgcttttcg aggaaggcca gggtggcgtc ggcctcggcg gagcggtatt
     1321 cgacgttctg gcgcacgtag gcgttgagga cttcgttgag caggcgctcg gcttcggcgc
     1381 ggtcggcgtc gctgtaggcg gcttcgatca cgttggactg gcgcgcgcgc tcgcgcactt
     1441 cgaggtcttc gcgcagggcg gcgatggcct ggatctggct gatgcgggcg agatcgaaat
     1501 gggtgccggg gcgggcgacg agctcggcga cgaagagcgt ggtcttgccg tcggcgctgc
     1561 tggcgggctg gccgacgctg ccgtcgagca ggggctggtc ctggtcgtcg tagagggtga
     1621 agccgccgtt gtcgccggcg atgagggtga gcggcaggcc gaccaggtag gacggcacct
     1681 cgagggtctg cacggtgatg cgctcgccgc cccaggcgta gctgcccagg ccgagcagcg
     1741 gtgcggcggg ctgctcgccg ttatagcggc gcgcgagggc gcggccgaag atggggaagt
     1801 agtcgggggt ggcgcggatg tcgaggcgca ggcgctcgac cacgcggccg aggatcatgc
     1861 gcgagcgcag gatctcgagc tcggcggtga ccgagctgtt ctcgccgagc acggcctcga
     1921 cgtcgcgcag cgcggccagc ccgcccttgg gccccttgct gtcctgcacc tgcaccagcc
     1981 cgtcggcgcg gtagatgggc gtggacacga aggccaccac cgcgccgatg aagacggcga
     2041 agaaggtgac ggcgaggatc agccacttgt attcgagcag gacggcgatg atctcgccca
     2101 ggttgatgaa gtcgtcccgg tcgtcgcgca tggacttggc gcggacagga ttcccgactt
     2161 cagccacggc ggagggtgca gtttctcgac gcaagggtgt aggttcctgt tgtgtggtca
     2221 tgtgttgcca atcaatttcc gtaggagcga cgcannnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnccattg cgcgccgcgg ctcatcgaac gcgacgatcg cgacttgcgt
     2401 cgctcctaca agaaccgtgc gaagcccgtt ccgacgcgag aatcgggccg gcgcggttcc
     2461 ttgttactgc cggccgccga acagcggata ccgcgtctcg ctggtgagct gcagcatctg
     2521 cgaggtcggc aggatgttgc tgaccacgcg gttccagcgc gcgatcgccg ccacgtcgac
     2581 atacaccaca tcccgcggca gcagcgggaa gcgctcggcc agcagcagcg cgtcggggga
     2641 gcgggcgttg aggtggaaga gctcggcgct gccgttgtcg ctgcgcatca catacaccca
     2701 ggccgggtcg gacgtgctct ggttgacgaa gccggcgtcg gacagcgcct cggcgagcgt
     2761 ggtgcggcgc ttgttcatga cgaacgagcc cgggttgcgc acctcgccga gcacgaagac
     2821 cttgttgagc tggcggtcgg gcacgttgat gatgtcgccc ggctgcagca gcacgttctg
     2881 gctgacgtcg ccctcctcgt acagcgcctg caggtcgacc cgccaggtct gcccgttgcg
     2941 ggtgagcagc acgttgccgt ggtcggcctc ttcggtgatg ttgccggcgc ggttgatggc
     3001 ctcgaccagg gtcatgcgga tgtcgtcgat cggctgcagg ccgggcttgg cgacttcgcc
     3061 gacgacatac acgcgcttgc tgcggaagga gacgatgcgc acgtcgagct ggacgttttc
     3121 gatgacgctg gccatgcgct tggcgaggac ctggcgcacc tcgcgggtgg tgaggccggc
     3181 gaccttcacc acgccggcga aggggaagaa gatggtgccg tcttcggcga ccagggtgcc
     3241 ggactgctcg gccgagcggt aggagccggc cgggatggtg atctcggggt ggtcccagac
     3301 gatgacgttg atgatgtcgc cggggccgag cttgtagtcc tggtagtcga agccggggat
     3361 cttcttggga tcgctcgcgc gggtggcggt ctggccgccg gccttgccgc tgcggaagcg
     3421 cgactccttg gcctggtgct gttcgatgat cagctcggcg gtgatgggct tgagcttgac
     3481 gttggccggg acggtgtcgg tgccctcggt gaccggcagc ggcacgctgg actcttcgcg
     3541 catgaggctg ccgctggtgc cggggatgag ggtgcagccg ccgatggccg cggcggcgaa
     3601 ggcgatcacg gcagtgcgga cgagaccgcc gctgcgggtg gcgtccgcac gggtggcggg
     3661 gccggccgcg cgggcgtcaa ggccactggc gtcggtggcg cgcacggcgc cattggatgc
     3721 gccgcagtgg gcgatccggt cggtggtgaa gcgactcatc gaaaaccccg ttcgagtttt
     3781 gcggcttgcc ccgcgcgcgc ctcggcggcg gcgtcgcgac atgccggcat gtttgcttgc
     3841 acgatatgcg tttcgggtgc cttgccgatg atgcccggat cgaggcgtgg agccggttcc
     3901 aggggggtca tcgcaggcaa ctcggcgccg tggtttacag caaaaaacgc gccagcaaat
     3961 aatgcattgc accaaaccag tgtcgagtca gtgcgttacg gagaggttaa agcccccgcg
     4021 ccgcgccgct ccgatctgct gtccgagcaa agtgtaaaga ttcccgacac cttgtggacc
     4081 atgccacgcg cagcccctga ggaactattc ctcattgctg cgttgcagca cccaagttgg
     4141 gcgaattgta ggggattacg ccagcatgcg caaaaccgcc cccgcccgga tcgcgactcg
     4201 tgtcgcctga tcgcgacttg cgtcgctcct acaccccctc ccgaagcgcg ccggttttcg
     4261 tgggagcgac gcaagtcgcg attgcagctt tcccgccccg accacgcctc tcgccgccgc
     4321 accgcctgat cgcgacttgc gtcgctccta caacacctcc cgaggcgcgc cggttttcgt
     4381 gggagcgacg caagtcgcga ttgcagcttt cccgccccga ccacgcccat cgccaccgca
     4441 ccgcctgatc gcgacttgcg tcgctcctac gaaaaccatg ccaacgcccg tggtccgatg
     4501 tcgttcagtg ccggcggcgg cgatcttcgg ccgtggcccg gtgcggttcg gcgctggcgg
     4561 ggccgatagg cggccttagc ggcaaaattg gctgaagctt gatcctggtc aacctttgtc
     4621 catattcacg ctgcgtttgc gcaacaagca cctagattgg aagcgaacag ttcgtgaaat
     4681 aagtcacggt tttccgactc taccgagaag agaccgcgac attggaagga gaccatcatg
     4741 tgtacattcg acgccccgat cgcccgttgc gaagccatga atcaaatggt gctgctggac
     4801 gaaacccgta gggaatgcgc tttcgaacat ggctgcccgc ccggtcagaa atgcccgctg
     4861 aacggctgct tcgctcgcca gagcggcctc tacgacgccc acccggaaat cggcgcgcac
     4921 ggccaaccgc gcgttgccca ccacgcacac tagggcccgg cccggcatcc gcgctccccg
     4981 ccgcaaggag ggggagcgct ttgccgcttc ccgttctccc cgcttcacga cttctcccgc
     5041 ctcctcgcct cctcgcctcc tcgtttcctc gtttccccgc ctccccgctt caccactgcc
     5101 ccgcttcacc gcttctccct cttctccgct tctcccgctt cttcaccccc tccccactcc
     5161 ccctccctcc gttttgcgga accccgcccg caagccgacg gtcagtctcc ggacagaaac
     5221 acg
//
LOCUS       DAOQNN010000222         5163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9690_length_5163_cov_9.351997, whole genome shotgun sequence.
ACCESSION   DAOQNN010000222 DAOQNN010000000
VERSION     DAOQNN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9690_length_5163_cov_9.351997"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1065)
                     /gene="galU"
                     /locus_tag="PKV97_11400"
     CDS             complement(160..1065)
                     /gene="galU"
                     /locus_tag="PKV97_11400"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNB06527.1"
                     /translation="MKKVTKAIFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVAAGITDMIFITGRTKRSIEDHFDKAYELETELEARGKKQLLEIVRQTVPKGVNCIY
                     IRQSEALGLGHAVRCASHVVSDEAFAVILADDLLDNKDGTPVLKQMVDVYNYHRCSVL
                     GTMNVPREETRSYGIVSTESDKGQVQRMTGIVEKPKPEEAPTTQAVVGRYILTPRIFD
                     HIRALKPGAGGEYQLTDAIASLLKEEAVLAFQFDGVRYDCGSKLGYLKATIEFGLRHP
                     ETGGDFAAYLSGRFGAEGKAAKAAK"
     gene            complement(1145..3226)
                     /gene="ligA"
                     /locus_tag="PKV97_11405"
     CDS             complement(1145..3226)
                     /gene="ligA"
                     /locus_tag="PKV97_11405"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNB06528.1"
                     /translation="MDLFAEDGRGETAADAAARAAALRAEIARHDHAYYVLDAPTIPD
                     AEYDRLFRELQAIERAYPALRSADSPTQRVGGKALAQFATVRHRVPMLSISTETDTEA
                     SGAFAFDARVRRALGLDEDDAAVAYAAELKFDGLAISLRYEDGVLVQAATRGDGETGE
                     DVTSNVRTVKAIPLRLLGEAPPVLEVRGEIYLRRDDFERLNARQAEAGEKIFVNPRNA
                     AAGSIRQLDPGIAARRPLSFYAYGLGEVAGWTLPATHTEVLDALAAFGLPVCGHRAQV
                     RGAEGLAAFHARIGALRDTLPFDIDGVVYKVDALALQQQLGFVTREPRWAVAHKYPAQ
                     EAVTLLRDIEVQVGRTGALTPVARLEPVFVGGVTVTNATLHNQDEIDRKDVRIGDWVI
                     VRRAGDVIPEVVAPILERRGGELPRFVLLDRFPTCPVCGSHVVRGEDEAVARCTGGLF
                     CPAQRKQALLHFAGRRAMDIEGLGDKLVDQLVDAAIVKTPVDLYRLGVLALANLERMG
                     EKSAQNLLMAIEKSRGTTLARFIFALGIRNVGEATARDLARHFGKLDALIAADVDALQ
                     QVPDVGPIVAKCIAEFFAEPHNREVIEQLRAAGVHWAEGEPQGSVAGALAGKTFVLTG
                     TLPTLSRDEAKARIEARGGKVAGSVSKKTHYVVAGAEAGSKLDKAQALGVAILDEDGL
                     RALLNETDTNA"
     gene            complement(3226..4308)
                     /locus_tag="PKV97_11410"
     CDS             complement(3226..4308)
                     /locus_tag="PKV97_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HNB06529.1"
                     /translation="MDSELQIALIGAGIAAVVLVVGYNKWQERRHRREAERAFKSEHR
                     DVLLEPREEPAAGERREPGIGAAEDEPRRFSEAPMKRSTPELPRLLDARADCVIRLET
                     IEALEVGRVWAIQAEQLAGLGKPVYWFGFNDAENVWQPLGPDSSGACHWFCAALQLVN
                     RQGSIGETDFMRFSGGVQRVADALMSLPPALPPRGETLRNATELDSFCADVDVQIGIN
                     VVSREGQFEGRAIHAVAEKHGLRLGADGAYHMVDGEHSVFTVANLESGRFSPESLKGL
                     VTRGLTLVIDVPRVSNGGAAFDRMMKMATTLAGELGGDVVDDNRAPFGAEAATIIRNQ
                     IGQFQSRMADHDIPAGSPLALRLFSL"
     gene            complement(4346..>5163)
                     /locus_tag="PKV97_11415"
     CDS             complement(4346..>5163)
                     /locus_tag="PKV97_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNB06530.1"
                     /translation="APVRARVAELRLALQAAELAVAQFEERLVEARADEAALAPLLAA
                     EPKESTLQREVARLAREIAELGAVNMAALDELTTASERKGYLDAQTEDLLQAIDTLED
                     AIRRIDRETREQLQDTYNTVNRQFGALFPQLFGGGRAELVLTGEEILDAGIQIVAQPP
                     GKKNASIHLLSGGEKALTAIALVFSMFQLNPAPFCMLDEVDAPLDDTNTERYANMVKR
                     MSAQTQFIFISHSKITMEFAQQLVGVTMQEQGVSRVVEVDIEEALRLADPAAA"
BASE COUNT          744 a         1777 c         1783 g          859 t
ORIGIN      
        1 acgacgtcgg ggcccgtggt ggcagcaacc gtggtcacgc gttcggaacc actctccccg
       61 ccacggacaa ggcagacctg ctcgagtacc tcaagacgat gtgaaacggg cgacccgcgg
      121 ccgcccgttt ccgacccgcc tcgacgcgcg agacgggact tacttggctg ccttcgccgc
      181 cttgccttcg gcgccgaagc gcccgctcag gtaggccgcg aagtcgccac cagtttccgg
      241 atggcgcaac ccgaactcga tcgtcgcctt caggtagccg agcttcgaac cgcagtcgta
      301 gcgcacgccg tcgaactgga aggccagcac ggcctcctcc ttgagcagcg atgcgatcgc
      361 gtcggtgagc tggtactcgc caccggcgcc gggcttcaac gcacggatgt gatcgaagat
      421 gcgcggggtc aggatgtagc gaccgaccac ggcctgggtc gtcggtgctt cctcgggctt
      481 gggcttctcg acgatgccgg tcatgcgctg cacctggccc ttgtcggatt cggtgctgac
      541 gatgccgtag ctgcgggttt cctcgcgcgg cacattcatc gtgccgagca ccgagcagcg
      601 gtgatagttg tacacatcca ccatctgctt caacaccggc gtgccatcct tgttgtcgag
      661 caggtcgtcg gcgaggatca ccgcgaaggc ctcgtcgctc accacgtggc tggcgcaacg
      721 cacggcatgg ccgaggccga gcgcctcgga ctggcggatg tagatacagt tgacgccctt
      781 cggcaccgtc tggcgcacga tctcgagcag ctgcttcttg ccgcgcgcct cgagctcggt
      841 ctcgagctcg taggccttgt cgaagtggtc ctcgatcgag cgcttggtgc gaccggtgat
      901 gaagatcatg tcggtgatgc cggcggcgac cgcctcttcc actgcgtact ggatcaaggg
      961 cttgtcgacg atcggcagca tctccttcgg gctcgccttg gtggcgggca ggaagcgcgt
     1021 acccatgccg gcaaccggga aaatcgcctt ggtgactttc ttcattttta ttgctctccc
     1081 atgaatagag ggggttgcga cgcaacagcg ggcgcgatca tgcggcaagc gtgcgcggtt
     1141 tgcatcaagc gtttgtatcc gtttcgttca gcagcgcgcg caggccgtcc tcgtcgagga
     1201 tcgcaacgcc gagcgcctgc gccttgtcga gcttggagcc cgcctcggcg ccggcgacca
     1261 cgtagtgcgt cttcttcgac accgagccgg cgaccttgcc gccgcgggcc tcgatccgcg
     1321 ccttggcctc gtcgcggctc agggtcggca gcgtgccggt gagcacgaag gtcttgcccg
     1381 cgagtgcgcc ggccaccgag ccctgcggct cgccctcggc ccaatgcacg ccggcggcac
     1441 gcaactgctc gatcacctcg cgattgtgcg gctcggcgaa gaactcggcg atgcatttcg
     1501 ccacgatggg ccccacgtcg ggcacctgct gcagggcatc gacgtcggcc gcgatcagcg
     1561 cgtcgagctt gccgaagtgg cgtgcgaggt cgcgcgcagt ggcctcgccg acattgcgga
     1621 tgccgagcgc gaagatgaag cgcgccagcg tggtgccacg gctcttctcg atcgccatca
     1681 gcagattctg cgccgacttc tcgcccatgc gctcgaggtt ggcgagcgcg agcacgccga
     1741 ggcggtagag gtcgaccggc gtcttcacga tcgccgcgtc cacaagctga tcgaccagct
     1801 tgtcgcccaa tccttcgatg tccatcgcac gccggccggc gaagtgaagc agcgcctgct
     1861 tgcgctgggc cgggcagaac agcccgccgg tgcagcgcgc gaccgcttcg tcttcgccgc
     1921 gcaccacatg cgaaccgcac accggacagg tggggaaccg gtcgagcagc acgaagcgcg
     1981 gcagttcgcc gccgcggcgc tccaggatcg gcgccaccac ctcggggatc acgtcgcctg
     2041 cacggcgcac gatcacccag tcgccgatgc gcacgtcctt gcggtcgatc tcgtcctggt
     2101 tgtgcagcgt ggcgttggtg accgtgacgc cgccgacgaa gaccggctcg aggcgcgcca
     2161 ccggggtcag cgcgccggtg cggccgacct gcacctcgat gtcgcgcagc agcgtcaccg
     2221 cctcctgagc agggtacttg tgcgccaccg cccagcgcgg ctcgcgggtg acgaagccga
     2281 gctgctgctg cagcgcgagc gcgtcgacct tgtagacgac gccgtcgatg tcgaagggca
     2341 gcgtgtcgcg cagtgcgccg atgcgtgcgt ggaaggccgc cagcccctct gcgccgcgca
     2401 cctgcgcgcg gtggccgcac accggcaggc cgaaggcggc gagcgcgtcc agcacctcgg
     2461 tatgggtcgc gggcagcgtc caaccggcga cctcgcccag cccgtaggcg tagaaggaca
     2521 agggccggcg cgcggcgatg ccggggtcga gctggcggat gctgccggcg gcggcgttgc
     2581 gcggattgac gaagatcttc tcgccggcct ccgcctggcg cgcgttgagg cgctcgaagt
     2641 cgtcgcggcg caggtagatc tcgccgcgca cctccagcac cggcggcgcc tcgccgagca
     2701 ggcgcagcgg gatcgccttc accgtgcgca cgttgctcgt gacgtcctcc ccggtctcgc
     2761 cgtcgccgcg ggtagcggcc tgcacgagca cgccgtcctc gtagcgcagg ctgatcgcga
     2821 ggccgtcgaa cttgagctcg gcggcgtagg ccaccgccgc atcgtcctcg tcgaggccga
     2881 gcgcgcggcg cacgcgcgca tcgaacgcga atgccccgct cgcctcggtg tcggtctcgg
     2941 tggagatcga cagcatgggc acgcggtggc gcaccgtggc gaactgcgcg agcgccttgc
     3001 cgcccacccg ctgtgtcggc gagtcggcac tgcgcagcgc cgggtacgcc cgctcgatgg
     3061 cctgcagctc gcggaacagg cgatcgtact cggcgtcggg aatcgtcggc gcgtcgagca
     3121 cgtaataggc atggtcgtga cgcgcgatct cggcgcgcag ggccgctgcg cgtgcggcgg
     3181 cgtcggccgc ggtctcgccg cggccgtctt cggcgaacag gtccatcaca aggagaacag
     3241 gcgcagggcg agcgggctgc cggcggggat gtcgtggtca gccatgcggc tctggaactg
     3301 gccgatctgg ttgcggatga tcgtcgccgc ctcggcgccg aagggcgcac ggttgtcgtc
     3361 gaccacgtcg ccaccgagtt cgccggcgag cgtggtcgcc atcttcatca tgcggtcgaa
     3421 ggcggcaccg ccgttgctca ctcgcggcac gtcgatcacc agcgtcagcc cgcgcgtcac
     3481 caggcccttg aggctctcgg gcgagaagcg accggactcg aggttggcca ccgtgaacac
     3541 gctgtgctcg ccatcgacca tgtggtaggc gccgtcggcc ccgaggcgca ggccgtgctt
     3601 ctcggccacg gcgtggatcg cgcggccttc gaactggcct tcgcgggaga cgacgttgat
     3661 gccgatctgc acgtcgacat cggcgcagaa gctgtcgagc tcggtggcgt tgcgcagggt
     3721 ctcgccgcgc ggcggcagcg ctgggggcag gctcatcagc gcatcggcca cgcgttgcac
     3781 gccgcccgag aagcgcatga agtcggtctc gccgatcgag ccctggcggt tgaccagctg
     3841 cagcgcggcg cagaaccagt ggcaggcgcc gctgctgtcg ggaccgagcg gctgccagac
     3901 gttctccgca tcgttgaagc cgaaccagta caccggcttg cccagcccgg cgagttgctc
     3961 ggcctggatc gcccacacac ggccgacctc gagcgcctcg atggtctcga ggcggatcac
     4021 gcagtcggcg cgcgcatcga gcaggcgtgg cagctcgggc gtgctgcgct tcatcggcgc
     4081 ctcgctgaag cggcgcggct cgtcttcggc ggcaccgatg ccgggttcgc gacgctcgcc
     4141 cgcggcgggc tcctcgcgcg gttcgagcag cacgtcacgg tgctcggact tgaaggcccg
     4201 ctcggcctcg cgtcggtggc ggcgctcctg ccatttgttg tagcccacca ccagcaccac
     4261 cgcggcgatg ccggcgccga tcagcgcgat ctgaagttcg ctgtccattt ggatcccaga
     4321 attgctccgt gtagcttgtc ctgcgtcacg ctgcggccgg gtcggccagg cgcagcgcct
     4381 cttcgatatc gacttccacc acccgcgaga cgccctgctc ctgcatcgtc acccccacca
     4441 gctgctgcgc gaattccatc gtgatcttgc tgtggctgat gaagatgaac tgcgtctgtg
     4501 cggacatgcg cttgaccatg ttcgcatagc gctcggtgtt cgtgtcgtcc agcggcgcat
     4561 ccacctcgtc gagcatgcag aatggggccg gattgagctg gaacatcgag aacaccagcg
     4621 cgatcgcggt gagtgccttt tccccgcccg agagcagatg gatcgacgcg tttttcttgc
     4681 ccggcggctg ggcgacgatc tggatgccgg cgtcgaggat ctcctcgccg gtcagcacca
     4741 gctccgcccg tccgccgccg aacaactgcg ggaacagggc gccgaactgc cgattgaccg
     4801 tattgtaagt gtcctgaagc tgctcgcgcg tctcgcgatc gatgcgccgg atggcatctt
     4861 ccagggtgtc gatggcctgc agcaggtctt cggtctgcgc gtcgaggtag cccttgcgct
     4921 cggaggcggt cgtgagctcg tcgagcgcgg ccatgttgac cgcgccaagc tcggcgattt
     4981 cgcgcgccag gcgggcgact tcgcgctgca gggtggattc cttgggctcg gcggcgagca
     5041 gcggcgcgag cgccgcctcg tcggcgcggg cctcgacgag gcgctcctcg aattgggcaa
     5101 ccgcgagttc ggcggcctgc agcgcgaggc gcagctcggc cacgcgcgcg cggaccggcg
     5161 cgg
//
LOCUS       DAOQNN010000223         5156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9712_length_5156_cov_11.490100, whole genome shotgun sequence.
ACCESSION   DAOQNN010000223 DAOQNN010000000
VERSION     DAOQNN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9712_length_5156_cov_11.490100"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PKV97_11420"
     CDS             <1..602
                     /locus_tag="PKV97_11420"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNB06531.1"
                     /translation="IDPTVHDIRFVEDDWENPTLGAWGLGWEVWLDGMEVTQFTYFQQ
                     VGGLDCKPVLGEITYGIERLAMYLQGVENVYDLVWSVAPDGRKVTYGDVYHQNEVEQS
                     TFNFEHSNVDFLFSLFNNYESEAKRVMEVGLALPAYEMVLKAAHTFNLLDARGAISVT
                     ERAAYIGRIRNLSRAVAQAYFESREALGFPMLNAEKEAA"
     gene            602..2689
                     /gene="glyS"
                     /locus_tag="PKV97_11425"
     CDS             602..2689
                     /gene="glyS"
                     /locus_tag="PKV97_11425"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNB06532.1"
                     /translation="MTVATLLVELLTEELPPKALPRLGETFATRIAEGLKAKGLAPVD
                     AAFRSFASPRRLAVTVAQVAAEAAAKEVTEKLMPVSVALDAEGKPTPALLKKMEAKGI
                     AVEAVARFERRLDGKAEALFHTAMVPGAKLADVLSGVVQDAIKALPIPKVMRWGDGDA
                     TFVRPVHKLTLLHGAEVVPGRVLDLDSGRTSRGHRFMSRGDISIATADAYEPTLLAEG
                     KVIPNFAERRADIERQLLAEAAKQGALLNEYADLLDEVAALVEHPTVYVGEFEAEFLA
                     VPQECLILTMRANQKYFPLFDGAGKLLNRFLIVSNMQVADPINIVAGNARVVRPRLSD
                     ARFFFEQDKKQKLEARLPRLAPVVYHNKLGSQLERVERLEALAGAIAAQLHGDVAAAK
                     RAARLAKADLVTDMVGEFPELQGIMGRYYALADGEGEVVADAIQAHYQPRFAGDALPA
                     GNVAAAVALADKLDALVGFFGIGMVPTGDKDPFALRRAALGVLRILMEAPLPLELPRL
                     IDTAAAGFKPGLLTAEGFAAQLQDFMYERLKNLLRETGRDGAVIDAVLALRPARIDLV
                     PAKLAAVEAFRALPEAEALAAANKRIVNILKKAEGAPGEPDLALLQEAAEKALFRQVV
                     EVAPLVRSHVANEDYTDALRALAGLRAAVDQFFEDVMVMAEEPLTRANRIALLAQLAG
                     LMNQVADISRLSA"
     gene            2748..3281
                     /gene="gmhB"
                     /locus_tag="PKV97_11430"
     CDS             2748..3281
                     /gene="gmhB"
                     /locus_tag="PKV97_11430"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HNB06533.1"
                     /translation="MKLIILDRDGVINVDSGQYIKSPEEWKPIPGALEAIARLNQWGW
                     RVVVATNQSGIGRGLFGMDTLNAINDRMVRSLAQAGGRLDGIFFCPHAADSTCDCRKP
                     KPGMLLQIAERFNVELADVPCVGDGLRDLQAAAAAGAQPWLVLTGKGETTRASGELPP
                     GTLVYPDLDAVAAALTA"
     gene            3340..4116
                     /locus_tag="PKV97_11435"
     CDS             3340..4116
                     /locus_tag="PKV97_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB06534.1"
                     /translation="MHLLRSILFALVLTVITVPYAFFATLIFWLPPMTRHRLITSWVP
                     IMMWVIRHVLGIRYRVIGAENIPAGPAVVLSKHQSAWETIALQVIFPPLCYVLKRELL
                     RVPFFGWALGRIPGIAIDRSAGKDALTQVVEQGRERLKEGLWVVVFPEGTRVAPGATL
                     RYKPGGAILAKRAGVPVVPVAHNAGEFWRRNAFIKRPGEIVVSIGPVIDVKGAKATDV
                     NEQAEAWIEGEMRRLFPHLYREEAGMPPAAGDAAAEDEGN"
     gene            4181..4555
                     /locus_tag="PKV97_11440"
     CDS             4181..4555
                     /locus_tag="PKV97_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNB06535.1"
                     /translation="MGEEIRALGEDEILASLAAGLPHWRLEAGALVRDYRTGGWKATL
                     MVAGVVGHLAEAAWHHPELELCWDRVRVRLSTHSPRGITARDFALARKIEEVVHWQPA
                     REGGALEGTPNADDRFRYIRYD"
     gene            complement(4667..>5156)
                     /locus_tag="PKV97_11445"
     CDS             complement(4667..>5156)
                     /locus_tag="PKV97_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNB06536.1"
                     /translation="TAFASAERLAALNLNTARSLLEDGVANTKALLAAKDVQELVNLQ
                     VSLAQPIVEKAVAYARSVYEISSQSQEEMSKVFEGQVAELNKGVATALDKAAKSAPAG
                     SDVAVAAVKSAIAAANSAYDSMSKAAKQVAEIAEANVAAATNATVKAVSTTAKTTAKK
                     AA"
BASE COUNT          787 a         1774 c         1784 g          811 t
ORIGIN      
        1 gcatcgaccc gacggtgcac gacatccgct tcgtcgagga cgactgggag aacccgaccc
       61 tgggcgcctg gggattgggc tgggaagtct ggctggacgg catggaggtc acccagttca
      121 cctacttcca gcaggtcggc ggcctcgatt gcaagccggt gctgggcgag atcacctacg
      181 gcatcgagcg cctggcgatg tacctgcagg gcgtggagaa cgtctatgac ctggtgtggt
      241 cggtcgcgcc ggacggtcgc aaggtcacct acggcgacgt ttaccaccag aacgaggtcg
      301 agcaatcgac gttcaacttc gagcacagca acgtcgactt cctgttctcg ctattcaaca
      361 actacgagtc cgaggccaag cgcgtgatgg aggtcgggct ggcactgccg gcctacgaga
      421 tggtgctcaa ggccgcccac accttcaacc tgctcgacgc ccgcggcgcg atctcggtga
      481 ccgagcgcgc cgcctacatc ggccgcatcc gcaacctctc gcgcgcggtc gcccaggcct
      541 atttcgaatc ccgcgaagcc ctcggcttcc cgatgctgaa tgctgaaaag gaggccgcgt
      601 gatgaccgtt gcgaccctgc tcgtcgaact gctcaccgaa gaactgccgc ccaaggccct
      661 gccgcgcctg ggcgagacct tcgccaccag gatcgccgag ggcctgaaag ccaagggact
      721 ggcgcctgtc gacgccgcct tccgcagttt cgccagtccg cgccggctgg cggtgaccgt
      781 ggcgcaggtg gctgccgagg ccgccgcgaa ggaggtcacc gaaaagctga tgcccgtgtc
      841 cgtggcgctc gacgccgagg gcaagcccac cccggcgttg ctgaagaaga tggaagcgaa
      901 gggcatcgcc gtcgaggccg tcgcccgctt cgagcgccgc ctggacggga aggccgaggc
      961 cttgttccac accgccatgg tgccgggcgc gaagctcgcc gacgtgctgt cgggggtcgt
     1021 gcaggacgcc atcaaggcgc tgcccatccc caaggtcatg cgctggggcg atggcgacgc
     1081 caccttcgtg cgcccggtgc acaagctcac cctgctgcac ggcgccgagg tggtgccggg
     1141 ccgcgtgctg gatctggatt ccggccgtac ctcccgcggc caccgcttca tgagccgcgg
     1201 cgacatctcc atcgccaccg ccgacgccta cgagcccacg ctgctcgccg agggcaaggt
     1261 catcccgaac ttcgccgagc gtcgcgccga tatcgagcgc cagctgctcg ccgaggccgc
     1321 gaagcagggt gcgctgctca atgaatacgc cgacctgctc gacgaggtcg ccgcgctggt
     1381 cgaacacccg acggtgtacg tgggcgagtt cgaggccgag ttcctcgccg tgccgcagga
     1441 gtgcctgatc ctgaccatgc gcgccaacca gaagtatttc ccgctcttcg atggcgccgg
     1501 caagctgctc aatcgtttcc tgatcgtctc caacatgcag gtggccgacc cgatcaacat
     1561 cgtcgccggc aacgcccgcg tggtgcgccc gcgcctgtcg gacgcgcgct tcttcttcga
     1621 gcaggacaag aagcagaagc tcgaggcccg cctgccgcgc ctggcgccgg tggtctatca
     1681 caacaagctc ggcagccagc tcgaacgcgt cgagcgcctc gaggcgctgg ccggtgcgat
     1741 cgctgcacag ctgcacggcg acgtcgccgc ggcgaagcgt gccgcgcgcc tggccaaggc
     1801 cgacctcgtc accgacatgg tcggcgagtt ccccgagctg cagggcatca tgggacgcta
     1861 ctacgcgctc gccgacggcg agggcgaggt ggtggccgac gccatccagg cccactacca
     1921 gccgcgcttc gccggcgacg cactgcccgc cggtaacgtc gccgccgcgg tggcgctggc
     1981 cgacaagctc gacgccctgg tcggcttctt cggtatcggc atggtgccca ccggcgacaa
     2041 ggatccgttc gcgctgcgcc gcgccgcgct cggcgtgctg cgcatcctca tggaagcgcc
     2101 gctgccgctc gagctgccca ggctgatcga caccgcggct gccggcttca agcccggcct
     2161 gctgacggcc gaaggcttcg ccgcgcagct gcaggacttc atgtacgagc gtctgaagaa
     2221 cctgctacgg gagacgggcc gtgacggcgc cgtgatcgac gccgtgctgg cgctgagacc
     2281 cgcgcgcatc gacctggtgc cggccaagct cgccgcggtg gaggccttcc gcgcgctgcc
     2341 cgaggccgag gccctggccg cggccaacaa gcgcatcgtc aacatcctca agaaggccga
     2401 aggcgcgccg ggcgagcccg acctcgcgct gctgcaggaa gctgccgaga aggcgctctt
     2461 ccgtcaggtg gtcgaggtgg cgccgctggt gcgttcgcac gtcgccaacg aggactacac
     2521 ggatgcgctg cgtgcactcg ccggactgcg cgcagcggtg gaccagttct tcgaagacgt
     2581 catggtgatg gcggaggagc cgctgacccg tgccaaccgc atcgcgctgc ttgcccagct
     2641 cgccggcctg atgaaccagg tggcggacat ctcgcgcctg tcggcctgat cgtcatagcc
     2701 cccgcggcgt gcgtcgccgc gggcttcgcc cccgcaagga gcccgtgatg aagctcatca
     2761 ttctcgaccg cgacggcgtg atcaacgtcg actcggggca gtacatcaag tcgcccgagg
     2821 agtggaagcc gatccccggc gcgctggagg cgatcgcacg gctcaaccag tggggctggc
     2881 gggtggtggt ggcgaccaat cagtccggca tcggccgtgg gctgttcggc atggacacgc
     2941 tcaacgccat caacgaccgc atggtcagga gcctcgccca ggcgggcggg cggctcgacg
     3001 gcatcttctt ctgtccgcac gcggccgact ccacctgcga ctgccgcaag cccaagccgg
     3061 gcatgctgct gcagatcgcc gagcgcttca acgtcgagct cgccgacgtg ccctgcgtcg
     3121 gcgacggcct gcgcgacctc caggccgccg cggcggctgg cgcgcagccc tggctggtgc
     3181 tcaccggaaa gggcgaaacc acgcgcgcga gcggcgagct tccgcccggt acgctggtgt
     3241 atcccgacct cgatgcggtc gccgctgcgc tgaccgcctg agcggcgcgg cgcgacagcg
     3301 ctgccgcatc cctcacgcac tcttcaccgg acgagaatcg tgcatctgct ccgctccatc
     3361 ctgttcgcac tcgtgcttac cgtgatcacg gtgccctacg cgttcttcgc caccctcatc
     3421 ttctggctgc cgccgatgac ccggcaccgg ctgatcacga gctgggtgcc gatcatgatg
     3481 tgggtgatcc gccacgtgct cggcatccgc taccgcgtga tcggcgccga gaacatcccg
     3541 gccggtcccg cggtggtgct gtccaagcac cagtcggcat gggagacgat cgcgctgcag
     3601 gtgatctttc cgccgctgtg ttacgtgctc aagcgcgagc tgctgcgcgt gcccttcttc
     3661 ggctgggcgc tgggcaggat cccgggcatc gccattgacc gctccgcagg caaggacgcg
     3721 ctcacccagg tggtggagca gggccgcgag cgcctcaagg agggcctgtg ggtggtggtc
     3781 ttccccgaag gcactcgggt ggcgccgggt gcgacgctgc gctacaagcc cggcggagcc
     3841 atcctcgcca agcgcgcggg cgtccccgtg gtgccggtgg cgcacaacgc gggcgagttc
     3901 tggaggcgca acgccttcat caagcgcccg ggcgagatcg tcgtcagcat cggcccggtg
     3961 atcgacgtca agggtgccaa ggccaccgat gtgaacgagc aggcggaggc ctggatcgaa
     4021 ggcgagatgc gccggctgtt cccgcatctc tatcgcgaag aggcgggcat gccgccggcc
     4081 gcgggcgatg ccgcggcgga agacgagggg aactgagcgc gttctcgccc ggtctcccgc
     4141 ataggccgcc ggggcggccc cagacatcgg gagagggacg atgggtgaag aaatccgcgc
     4201 actcggcgag gacgagatcc tcgccagcct tgcggcgggg ctgccgcact ggcgcctcga
     4261 ggccggcgcc ctggtgcgcg actaccgcac cggcggctgg aaggcgacac tgatggtggc
     4321 cggtgtggtc gggcacctgg ccgaggcggc ttggcatcat cccgagctgg agctgtgctg
     4381 ggaccgggtg cgggtgcgcc tgtccacgca ttcgccgcgc ggcatcaccg cacgcgattt
     4441 cgcgctcgcg cgcaagatcg aggaggtcgt gcactggcag cccgcgcgcg agggcggggc
     4501 gctggaaggc acgccgaacg cggacgaccg cttccgttac atccgctacg actgaagcgg
     4561 cgtgcgtgcg tttccgctgc agtccgcgcc ctgccgcgtc accagtcggt agacgcaaaa
     4621 aggccccgca tgcggggcct ttttgtcaga cggtgccgga ggaagcttag gcagccttct
     4681 tggcggtggt cttggcagtg gtgctgacgg ccttgacggt ggcgttggtc gcggcagcca
     4741 cgttggcttc ggcgatctcg gcgacctgct tggcggcctt gctcatgctg tcgtaggcgc
     4801 tgttggcggc ggcgatggcc gacttgacag cggcgacggc gacgtccgaa ccggcgggag
     4861 ccgacttggc agccttgtcg agcgcggtgg cgacgccctt gttgagctcg gcgacctgac
     4921 cttcgaagac cttcgacatc tcttcctggc tctgcgacga gatctcgtag acgctgcggg
     4981 cgtaggcgac ggccttctcg acgatcggct gggccagcga aacctgcagg ttcaccagct
     5041 cttgcacgtc cttggcagcc agcagggcct tggtgttggc cacgccgtct tccagcagcg
     5101 aacgggcggt gttcaggttc agggcggcga ggcgctcggc gctggcgaag gcggtg
//
LOCUS       DAOQNN010000224         5150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9727_length_5150_cov_9.223356, whole genome shotgun sequence.
ACCESSION   DAOQNN010000224 DAOQNN010000000
VERSION     DAOQNN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9727_length_5150_cov_9.223356"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /gene="flhF"
                     /locus_tag="PKV97_11450"
     CDS             <1..1033
                     /gene="flhF"
                     /locus_tag="PKV97_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNB06537.1"
                     /translation="TAARPAPSGPAVFSGRAARSDADEFGSARVRELQETNARLMAEL
                     TGIRGMIERQLAGFAWGETRRQAPARAAMLGELLEAGFSAAIARKLVEAVHEDADRIE
                     AREAVGAALDRLMRACASDEDLLDRGGVYALVGPTGVGKTTTTAKLAARCVVRHGAGR
                     LALITTDGYRIGAQEQLRIYGRILGVPVFSVRDAADLRQTLAGLRNKHMVLIDTMGMS
                     QRDRMVAEQAAMLSGAGDVRRLLLLNATARGDTLDDVVRAYAGGDLAGCIFTKVDEAA
                     SLAPALDVAVRHELDIRYLTNGQRVPEDLHLPNRAYLLHRALREHGSASPWHLQGDEA
                     GMLLAAGGA"
     gene            1036..1821
                     /locus_tag="PKV97_11455"
     CDS             1036..1821
                     /locus_tag="PKV97_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FleN"
                     /protein_id="HNB06538.1"
                     /translation="MLDGREDQAAGLRRLFRRAPPQVVALYAGGRRAADNAALAAHRI
                     AGRSERVLVLDEAEGGARLCGALGVGEGVDLLQLLDGRSTLADIVQPVPGLVGRVPAA
                     AAALALPLLDDPHRACLIEALRILHRHAGFVLVHASGEQAAEPSPFVLAAPRRLVVAE
                     ASASGATDAYHTIKGLAAAGAGSVHVAVCRARGRADATAFFRSLDALVRRHVGVPLAW
                     VGELERDEIAGGLQQPLQASTHDAGAAFLRRVGAMARAGVMRA"
     gene            1946..2719
                     /locus_tag="PKV97_11460"
     CDS             1946..2719
                     /locus_tag="PKV97_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HNB06539.1"
                     /translation="MYDAHGKLDKGGLVENYAPLVKRIAYQLMSRLPASVDVDDLIQN
                     GMMGLLDALGRYEQGLGAQFETYAVQRIRGAMLDGLRENDWLPRSLRRDMRRIEAAIH
                     SLEQQNGRPPSESELAAALDLPLEDYQRMLQDARGHQLVYLEDFNREEDDDYLDRHLA
                     QEGNDPLTLLEDTDLRRRLVGAIEHLPEREQLVMALYYDEELNLREIGEVLGVTESRV
                     CQLHSQAVARLRACVFEGGTAMPVARRRGRPPKNAAAGR"
     gene            2750..3490
                     /locus_tag="PKV97_11465"
     CDS             2750..3490
                     /locus_tag="PKV97_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNB06540.1"
                     /translation="MDKISLVGLTLGIAAILVGQVIEGGHLSSLFQPTAFMIVIGGTL
                     GAVMLQSPLRIFMQGMRMARWVFVPPAPAFDTIIEQVANWGHTARKEGLLSLENQIEA
                     LSDPFMRKGLQLLVDGIEPNRLREVLEVEIGVWENQMKMSARIWEAAGGYAPTIGILG
                     AVMGLIHVMENLSDPSRLGAGIAVAFVATIYGVGFANLVFLPIAKKLGAHIVTLTTQR
                     EMFVDGLVGIANGDNPRIIASRMQGYLA"
     gene            3506..4345
                     /gene="motD"
                     /locus_tag="PKV97_11470"
     CDS             3506..4345
                     /gene="motD"
                     /locus_tag="PKV97_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HNB06541.1"
                     /translation="MRRRRKLDEEHDNHERWLVSYADFITLLFAFFVVMYSLSSVNEG
                     KYRVLSDSLVQAFRNVNVNESGEQIVLPKVSVVPSQPITRPAPPAVDPVVEQRRQQAA
                     QRMRNVADEIRRVLSPLTGSGQVKVTEGAFGVSVEINASLLFAPGEAALGSNAVSALQ
                     AVARVLAPAEFPITVEGHTDNVPISTFRFPSNWELSAVRASTVARLFIDNGVTPARLT
                     AAGYADQRPVADNADEEGRGRNRRVTILIESRMADPEGNAGPTTIPPDDPIRSILPEA
                     PQG"
     gene            complement(4608..>5150)
                     /locus_tag="PKV97_11475"
     CDS             complement(4608..>5150)
                     /locus_tag="PKV97_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNB06542.1"
                     /translation="KVSELPGGRGDGAPITTLVEFQDGGKLAQVVTDTPDSVYFFANS
                     GGYGFLCSVADASSRQRAGKAFMSLEKGEKVLAPAKVFGDWIAAASENGRILVFPRPE
                     MKTQSGGRGVIVMALDEGEALAAMAVPADDAVLAVEGSGRGGKALTIELKPAQREPLR
                     HRRARKGVPLTPKLKPERMR"
BASE COUNT          734 a         1883 c         1794 g          739 t
ORIGIN      
        1 gaccgctgcc cgcccggcgc cgagcggtcc ggcggtgttc tccggtcgtg ccgcgcgttc
       61 cgacgccgac gaattcggca gcgcgcgcgt gcgcgaactg caggagacca acgcccgcct
      121 gatggccgag ctcaccggca tccgcggcat gatcgaacgt cagctcgccg gcttcgcctg
      181 gggcgagacg cgccgccagg cgccggcgcg cgcggccatg ctcggagagc tgctcgaggc
      241 cgggttctcc gcggcgatcg cgcgcaagct cgtcgaggcc gtgcacgagg acgccgaccg
      301 catcgaggcg cgcgaggccg tgggtgccgc cctcgatcgc ctgatgcggg cctgcgcctc
      361 ggacgaggac ctgctcgacc gtggcggcgt gtatgccctg gtcggcccca ccggcgtcgg
      421 caagaccacc accaccgcca agctcgccgc ccgctgcgtg gtgcgccacg gcgccggccg
      481 gctcgcgctg atcaccaccg acggctaccg catcggcgcc caggagcagc tgcgcatcta
      541 cggccgcatt ctcggcgtgc cggtgttctc ggtgcgcgac gcggccgacc tgcgccagac
      601 cctggccggc ctgcgcaaca agcacatggt gctcatcgac accatgggca tgagccagcg
      661 cgaccgcatg gtggccgagc aggcggccat gctctccggc gccggcgacg tgcgccggct
      721 gctgctgctc aacgccaccg cgcgcggcga caccctggac gacgtggtgc gcgcctatgc
      781 cggcggcgac ctcgccggct gcatcttcac caaggtggac gaggccgcct cgctcgcgcc
      841 ggcgctcgat gtcgcggtgc gccacgagct cgacatccgc tacctcacca acggccagcg
      901 cgtacccgag gacctgcacc tgcccaaccg tgcctacctc ttgcatcgcg cgctgcgcga
      961 gcacggcagc gcctcgccct ggcacctgca gggcgacgag gccggcatgc tgctcgcggc
     1021 gggaggggcc tgagcatgct cgacggacgc gaagaccagg ccgccggcct gcgcaggctg
     1081 ttccgccgcg cgccgcccca ggtggtggcg ctgtatgcgg gcggtcgccg cgccgccgac
     1141 aacgccgcgc tcgccgccca ccgcatcgcc gggcgcagcg agcgagtgct ggtgctggac
     1201 gaggccgagg gcggcgccag gctgtgtggc gcgctcggcg tgggcgaggg cgtggacctg
     1261 ctgcagctgc tcgacggccg cagcacgctc gccgacatcg tgcagccggt gccgggcctg
     1321 gtgggccgcg tgccggccgc ggccgcggcg ctcgcgctgc ccctgctcga cgatccgcac
     1381 cgtgcctgcc tgatcgaggc gctgcgcatc ctgcaccgcc acgccggctt cgttctggtg
     1441 cacgcgagcg gcgaacaggc cgcggagccg tctccgttcg tgctcgccgc gccgcgccga
     1501 ctggtggtcg ccgaggcgag cgcgagcggc gccaccgacg cctaccacac catcaagggc
     1561 cttgccgccg cgggagccgg ctcggtgcac gtggccgtgt gccgcgcgcg cgggcgggcg
     1621 gacgccaccg ccttcttccg cagtctcgat gcgctcgtgc gccgccacgt gggcgtgccg
     1681 ctcgcgtggg tgggcgagct cgaacgcgac gagatcgccg gcggcctgca gcagccgctg
     1741 caggcttcga cccacgatgc gggcgcggcc ttcctgcgcc gggtcggcgc catggcgcgt
     1801 gccggcgtca tgcgcgcctg atgcgccgcc gacgtgacag atcgcgctgg taaaggcggc
     1861 gatgctacgg gaaaatgcac aactcctatc cacgcgggcg gggtccgacg gtcccgtctg
     1921 ctgccagaac ccacaggccc gatgcatgta cgacgcacac ggtaagctcg acaagggtgg
     1981 cctcgtcgag aactacgccc cgctggtcaa gcgcatcgcc taccagttga tgtcgcgcct
     2041 gcccgccagc gtggatgtgg acgacctgat ccagaacggc atgatgggcc tgctcgatgc
     2101 cctcggccgc tacgagcagg gcctcggcgc ccagttcgag acctatgcgg tgcagcgcat
     2161 ccgcggcgcc atgctcgacg gcctgcgcga gaacgactgg ctgccgcgca gcctgcgccg
     2221 cgacatgcgc cgcatcgagg cggcgatcca cagcctggag cagcagaacg gccgcccgcc
     2281 ctccgagagc gagctcgccg ccgcgctcga cctgccgctg gaggattacc agcgtatgct
     2341 gcaggacgcg cgcggccacc agctcgtcta tctcgaggat ttcaaccgcg aggaagacga
     2401 cgactatctc gaccgccatc tcgcccagga gggcaacgat ccgctcaccc tgctcgagga
     2461 caccgacctg cgccgccgcc tggtcggggc gatcgagcac ctgcccgagc gcgagcagct
     2521 ggtgatggcg ctgtattacg acgaggagct caacctgcgc gagatcggcg aggtgctcgg
     2581 cgtcaccgag tcgcgcgtgt gccagctgca cagccaggcg gtggctcgcc tgcgcgcctg
     2641 cgtcttcgag ggcggcacgg cgatgccggt cgcgcgccgg cgcgggcgcc cacccaagaa
     2701 cgcggccgcg ggccgctgat cggggccgcc agcgctgccc ccggatacca tggacaagat
     2761 cagcctcgtc ggcctcaccc tcggaatcgc cgccatcctc gtcggccagg tgatcgaggg
     2821 cggtcacctg tcctcgctgt tccagcccac cgccttcatg atcgtgatcg gcggcacgct
     2881 cggcgcggtc atgctgcaga gcccgctgcg catcttcatg cagggcatgc gcatggcgcg
     2941 ctgggtcttc gtgccgccgg cgcccgcctt cgatacgatc atcgagcagg tcgcgaactg
     3001 gggccacacc gcgcgcaagg agggcctgct gagcctggag aaccagatcg aggcgctgtc
     3061 cgaccccttc atgcgcaagg gcctgcagct gctggtcgac ggcatcgagc ccaaccgcct
     3121 gcgcgaggtg ctcgaggtcg agatcggcgt gtgggagaac cagatgaaga tgtcggcgcg
     3181 catctgggag gcggccggcg gctatgcgcc gacgatcggc atcctgggcg cggtgatggg
     3241 cctgatccac gtgatggaga acctctccga cccttcgcgg ctgggcgccg gcatcgcggt
     3301 cgccttcgtc gccacgatct acggcgtggg cttcgccaac ctggtgttcc tgccgatcgc
     3361 caagaagctg ggcgcgcaca tcgtcaccct gaccacccag cgcgagatgt tcgtcgatgg
     3421 cctggtgggg atcgccaacg gcgacaaccc gcgcatcatc gccagccgca tgcaaggcta
     3481 cttggcctga gcgccgggag acatcatgcg gcgcaggcgc aagctcgacg aggaacacga
     3541 caaccacgaa cgctggctgg tgtcctacgc cgacttcatc acgctgctgt tcgccttctt
     3601 cgtggtcatg tattcgctgt cgtcggtcaa cgagggcaag taccgcgtgc tctccgactc
     3661 gctcgtgcag gccttccgca acgtcaatgt gaacgagagc ggcgaacaga tcgtgctgcc
     3721 caaggtcagc gtcgtgcctt cgcagccgat cacccggccg gcgcccccgg cggtggatcc
     3781 tgtcgtggag cagcgtcgcc agcaggccgc gcagcgcatg cgcaacgtgg ccgacgagat
     3841 ccgccgcgtg ctctcgccgc tgacggggtc cggccaggtg aaggtcaccg agggcgcctt
     3901 cggcgtcagc gtcgagatca acgccagcct gctcttcgcg ccgggcgagg ccgcgctcgg
     3961 cagcaatgcc gtgtcggcgc tgcaggcggt ggcgcgggtg ctggcgccgg cggagttccc
     4021 gatcacggtc gagggccaca ccgacaacgt gccgatcagc accttccgct ttccgtcgaa
     4081 ctgggagctc tcggcggtgc gtgcctcgac ggtggcgcgg ctcttcatcg acaacggcgt
     4141 caccccggcg cgcctgaccg ccgccggcta tgccgaccag cgcccggtcg cggacaacgc
     4201 ggacgaggag ggccgcggcc gcaaccgtcg cgtcaccatc ctgatcgagt cgcgcatggc
     4261 cgaccccgaa ggcaatgccg gcccgaccac catcccgccc gacgacccca tccgctccat
     4321 cctgccggag gccccgcagg gttgatggca gccggtcggg gtgagacccc acggcgtgtc
     4381 cggttccttg cggatctcgt gggagcgacg ccaaggtgca ttggtacgtc gctcccacga
     4441 gagatgccga ggtgcattgg tgcgtcgctc ccacgagaga tgccgaggtg catcggtacg
     4501 tcgctcccac gagagacgct gaggcgcatc ggtgcgtcgc ttccacgcga gatacgaggc
     4561 gcgtcggtgc gccgtaggag cgacgcgagt cgcgatcggc gcaacggtca gcgcatgcgc
     4621 tcgggcttga gcttcggagt cagcggcacg cccttgcgtg cgcggcggtg gcgcagcggt
     4681 tcgcgttggg cgggcttgag ctcgatcgtc aacgccttgc cgccgcgacc actgccctcg
     4741 acggcgagca ccgcatcgtc ggccggcacc gccatcgccg ccagcgcctc gccttcgtcg
     4801 agcgccatca cgatgacgcc gcggccgccg ctctgggtct tcatctcggg gcgggggaag
     4861 accaggatgc ggccgttctc ggacgccgcc gcgatccagt cgccgaacac cttcgccggc
     4921 gccagcacct tctcgccctt ttccaggctc atgaaggcct tgcccgcgcg ctgacggctg
     4981 ctggcgtcgg cgacgctgca caggaaacca tagccgccgc tgttggcgaa gaagtacacc
     5041 gaatccggcg tgtcggtgac gacctgggcg agcttgccgc cgtcctggaa ctcgaccagc
     5101 gtggtgatcg gcgcgccgtc gccgcgcccg ccgggcagct cggacacctt
//
LOCUS       DAOQNN010000225         5144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9741_length_5144_cov_9.608371, whole genome shotgun sequence.
ACCESSION   DAOQNN010000225 DAOQNN010000000
VERSION     DAOQNN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9741_length_5144_cov_9.608371"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..580
                     /gene="amrA"
                     /locus_tag="PKV97_11480"
     CDS             14..580
                     /gene="amrA"
                     /locus_tag="PKV97_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNB06543.1"
                     /translation="MPDTDFGTTLLALARAAIAHRLGRGPAPVIPDDPRLHEKGATFI
                     TLAKDGELRGCIGSLRRSRELGEDVIHNALAAAFEDSRFEPLAPEELGAVAIEVSILS
                     EPVFLDFFGEEELLAQLRPGEDGLIIFSGCHSATFLPQVWEQLPEPRQFLAALKHKAG
                     LPTNREVVELMAARYHVEKFKETEREPS"
     gene            577..1701
                     /gene="amrS"
                     /locus_tag="PKV97_11485"
     CDS             577..1701
                     /gene="amrS"
                     /locus_tag="PKV97_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNB06544.1"
                     /translation="MNTKPAPAAEVGSRHPARYWHRIEDGRYQCDLCPRLCKLHPGQR
                     GACFVRMREGDEMLLTTYGRSSGFCIDPIEKKPLAHFYPGSKVFSFGTAGCNLACKFC
                     QNWDISKSRDLDTLMDEASPAEIAETALRWGCHSVAFTYNDPVIFAEYAMDVADACHA
                     RGLKTVAVTAGYISECARRDFFSRMDAANVDLKGFTDDFYVKLCGAHLQPVLDTLVWL
                     RHETDVWVELTTLLIPGHNDSASEVTALANWVRKELGAEVPLHFSAFHPDFKMDELPP
                     TPPATLARARRIALDAGLKHVYTGNVHDIEGDTTRCASCGEPLIVRDWYEIRRYALGN
                     DGHCPHCGSVLAGRFAAVGAAVGDAFGARRIPVAIHRHGT"
     gene            1720..2376
                     /locus_tag="PKV97_11490"
     CDS             1720..2376
                     /locus_tag="PKV97_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB06545.1"
                     /translation="MKLRNTLISIPAQGAWLDGLLAHAPDVRGLAILPQSSGHPALDS
                     GPRPVDVALQSRGFATLRVDLLTRQEALRDPDASYNIARLTERILAAVEWIAHQPPLA
                     GLQLGLVTQDTGCAAAIRAAVKAPETFAAIACVDGRADLAGAEPLRQLRCPARFIVSP
                     GAPESAILEKAFSLLGGARHWLALPDAGDARQQEILQARAVTSWLEQHLAAAERTRVE
                     "
     gene            2406..3029
                     /locus_tag="PKV97_11495"
     CDS             2406..3029
                     /locus_tag="PKV97_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HNB06546.1"
                     /translation="MSISSLGGGDAAWRQGTAMNAARPAGASWGPATAGAGGKAQEAG
                     AGAADTLSEAEQRQVAELAERDREVRAHEMAHVAAGAGLVTRGASYTYQTGPDGQRYA
                     IGGEVGIDTSPGRSPEESLSKAERIRAAALAPAEPSGQDRQVAAQASRMASDARMEIA
                     RREFEQGGAGTEAAATRDGPGYRGMRAAQAYSAALAPGQGPTLSATA"
     gene            3188..>5144
                     /locus_tag="PKV97_11500"
     CDS             3188..>5144
                     /locus_tag="PKV97_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNB06547.1"
                     /translation="MSLSLDALLSRLATVKLPKPGGRLELEPLAGSADALAIAQLASR
                     GRMLVVITANPLDAQRLQDEIAWVAPGLRAHLLPDWETLPYDSFSPHQDLISERLSTL
                     YAISRGETDIVLVPASTALYRMAPPGYLAAYTFFLKQGERLDVEQFKAQMALAGYAHV
                     TQVVSPGEFSVRGGLVDLFPMGSPLPFRIDLFDDEVESIKTFDPDTQRTVYPTKEIRL
                     LPAREFPLDDKGRTRFRSRFRETFEGDPTRASIYKDVSNGIAPAGIEYYLPLFFDETA
                     TLLDYLPAETPVLLHRDVPAAIAEFWKDTRSRHDLLKGDRSRPVLAPEQLFLTDEAFF
                     IALKNLPRLAIGAEVGAKAGAEPAAASTDALALPPPEVAVERKATDPLHKLKAFLDGF
                     DGRVLLLSDSPGRRETMAEFFAEYDVRPEATADFGAFLDSDARLALGVAPLARGFVLP
                     AARLAVLTETELYASTARSRVRRDARKAATMEGWLRDLSELKIGDPVVHVSHGIGRYL
                     GLLHMNLGEGDTEFLHLEYNGGDKLYVPVSQLHVITRYAGADPDAIDLHRLGSGQWEK
                     AKKKAAMQVRDTAAELLALYAQRAARPGHRFDFKQHDLEAFAEAFGFETTPDQQAAID
                     AVVADMKSGRPMDRLVCGDVGFGKTEVA"
BASE COUNT          769 a         1981 c         1667 g          727 t
ORIGIN      
        1 gaacccccaa gccatgcccg acactgattt cggtaccacc ctgctcgcgc tcgcccgcgc
       61 cgcgatcgcc caccgcctcg gccgtggccc cgccccggtc atcccggacg atccccgcct
      121 gcacgagaag ggcgcgacct tcatcaccct cgccaaggac ggcgagctgc gcggctgcat
      181 cggcagtctg cggcgctcgc gcgagctcgg cgaggacgtg atccacaacg cgcttgcggc
      241 ggccttcgag gactcgcgct tcgagccgct cgcgcccgag gaactcggcg cggtggcgat
      301 cgaggtctcc atcctgtccg aaccggtgtt cctcgacttc ttcggtgagg aagagctgct
      361 cgcccagctg cgccccggcg aggacgggct gatcatcttc tccggctgcc acagcgccac
      421 cttcctgccc caggtgtggg agcagctgcc cgagccgcgc cagttcctcg ccgcgctcaa
      481 gcacaaggcc ggcctgccca ccaaccgcga ggtcgtcgag ctgatggccg cgcgctatca
      541 cgtggaaaaa ttcaaggaga ccgaacggga gccctcatga acacgaagcc cgcgccagcc
      601 gccgaggtcg gcagccgcca tcccgcgcgc tactggcacc gcatcgaaga cggtcgctac
      661 cagtgcgacc tctgtccgcg cctgtgcaag ctgcaccccg gccagcgcgg ggcctgcttc
      721 gtgcgcatgc gcgagggcga cgagatgctg ctcactacct atggacgcag ctcgggcttc
      781 tgcatcgacc cgatcgagaa gaagccgctc gcccacttct acccgggcag caaggtgttc
      841 tccttcggca ccgccggctg caacctggcg tgcaagttct gccagaactg ggacatctcc
      901 aagtcgcgcg acctcgacac cctgatggac gaggcctcgc ccgcggaaat cgccgagacc
      961 gcgctgcgct ggggctgcca cagcgtcgcc ttcacctaca acgacccggt gatcttcgcc
     1021 gagtacgcga tggacgtggc cgacgcctgc catgcgcgcg ggctgaagac cgtggcggtg
     1081 accgccggct acatctccga gtgcgcgcgg cgcgatttct tctcgcgcat ggatgcggcc
     1141 aacgtcgatc tcaagggctt caccgacgac ttctacgtca agctgtgcgg cgcccatctg
     1201 cagcccgtgc tcgacaccct ggtatggctg cgccacgaga ccgacgtgtg ggtcgaactc
     1261 accaccctgc tgatcccggg ccacaacgac tccgccagcg aggtcaccgc gctggcgaac
     1321 tgggtgcgca aggagctggg cgccgaggtg ccgctgcact tcagcgcctt ccaccccgac
     1381 ttcaagatgg acgagctgcc gcccacgccg cctgccacgc tcgcgcgcgc gcgccgcatc
     1441 gccctcgacg ccggcctgaa gcacgtctac accggcaacg tgcacgacat cgaaggcgac
     1501 acgacccgtt gcgcgagctg cggcgagccc ctgatcgtgc gcgactggta cgagatccgc
     1561 cgctacgccc tcggcaacga tggccactgt ccgcactgcg gcagcgtgct cgccggtcgc
     1621 ttcgctgcag tgggcgccgc ggtcggcgac gccttcggcg cgcgccgcat cccggtcgcc
     1681 atccaccgtc acggcacctg agcgcattcg acccgcaacg tgaaacttcg aaacaccctc
     1741 atcagcatcc ccgcacaggg cgcgtggctc gacggcctgc ttgcacacgc gccggacgtg
     1801 cgcgggctcg ccatcctccc gcagtcgagc ggacaccctg cgctcgacag cgggccccgg
     1861 ccggtcgatg tcgccctgca gtcgcgcggc ttcgccactc tccgggtcga cctgctgacc
     1921 cggcaggagg cgctgcgcga ccccgatgcg tcgtacaaca tcgcccgcct gaccgagcgc
     1981 atcctcgcgg cggtcgagtg gatcgcccac cagccaccgc tcgccgggct gcagctcggc
     2041 ctcgtcacgc aggacaccgg ctgcgccgcc gcgatccgcg cggcggtcaa ggcgccggag
     2101 accttcgcgg cgatcgcctg tgtcgacggc cgcgccgacc tcgcgggtgc cgagcccttg
     2161 cgccagctgc gctgcccggc gcgcttcatc gtcagccccg gcgccccgga gagcgcgatc
     2221 ctggagaaag ccttttccct gctcgggggg gcgcgccact ggctcgccct gcccgacgcc
     2281 ggtgacgccc gccagcagga gatcctgcag gcgcgggcgg tgacgagctg gctcgaacag
     2341 cacctcgccg cggccgagcg cacgcgggtg gaataggatc ggagcgcgcc cgcttcatgt
     2401 ccgcaatgtc gatttcctcg ctcggtggcg gtgacgcggc gtggcgccag ggcacggcga
     2461 tgaatgccgc ccgccctgcc ggcgcctcct gggggccggc gacggcgggg gccggcggca
     2521 aggcgcaaga ggcaggagcg ggggcggccg acacgctctc cgaggccgaa cagcgccagg
     2581 tcgccgagct cgcggaacgc gaccgcgagg tccgtgccca cgagatggcc catgtcgccg
     2641 cgggcgccgg cctggtgacc cggggcgcca gctacaccta tcagaccggc ccggacggcc
     2701 agcgctacgc gatcggcggc gaggtcggga tcgatacctc gccgggccgc agccccgagg
     2761 agtccctctc caaggccgag cgcatccgcg ccgcagccct cgcccccgcc gagccgtcgg
     2821 gccaggaccg ccaggtcgcc gcgcaggctt cccgcatggc aagcgatgcg cgcatggaga
     2881 tcgcccgccg cgaattcgag caaggcggcg cgggcaccga ggcagcggcc acgcgcgatg
     2941 gccccggcta tcgcggcatg cgagcggcgc aggcctattc ggcagcgctc gctccaggcc
     3001 aggggcccac cctttcagcc accgcctgag ccttccgctc cgcgttcgag cgatcacgcc
     3061 gtgcgctggc gggccgggat gaacgataat tctctctttg ccggccccgc gtccctaccg
     3121 ccatgctgcc cgcctccgcc acggtgacgg agggtcgcag tccgcagacg acgctcgaga
     3181 aaccacgatg tccctctccc tcgacgccct cctctcccgg ctcgccacgg tcaagctgcc
     3241 caagcccggc ggccggctcg aactcgagcc gctcgccggc tcggcagacg ccctcgccat
     3301 cgcccagctc gccagccgcg gccgcatgct ggtggtgatc acggccaacc cgctcgacgc
     3361 ccagcgtctg caggacgaga tcgcctgggt cgcacccggc ctgcgcgcgc acctgctgcc
     3421 ggactgggag accctgccct acgacagctt ctcgccccac caggacctga tctccgagcg
     3481 gctgtcgacg ctctatgcga tttctcgcgg cgaaaccgac atcgtgctgg tgccggcctc
     3541 caccgcgctc tatcgcatgg caccaccggg ctacctcgcc gcctacacct tctttctcaa
     3601 gcagggcgag cggctggatg tggagcagtt caaggcgcag atggcgctcg caggctatgc
     3661 gcacgtcacc caggtggtga gcccggggga gttctcggtg cgcggcgggc tggtggacct
     3721 gttcccgatg ggttcgccgc tgcccttccg tatcgacctc ttcgacgacg aggtcgagag
     3781 catcaagacc ttcgaccccg acacccagcg cacggtgtat cccaccaagg agatccgcct
     3841 gctgccggcg cgcgagttcc cgctcgacga caaggggcgc acgcgctttc gcagccgctt
     3901 ccgcgagacc ttcgagggcg acccgacgcg cgcgtcgatc tacaaggacg tctccaacgg
     3961 catcgccccc gccggcatcg agtactacct gccgctgttc ttcgacgaga ccgcgacgct
     4021 gctggactac ctgcccgccg agacgccggt gctgctgcac cgcgacgtgc cggcggcgat
     4081 cgccgagttc tggaaggaca cgcgctcgcg ccacgacctg ttgaagggcg accgcagccg
     4141 cccggtgctg gcaccggagc agctcttcct gaccgacgag gccttcttca tcgcgctgaa
     4201 gaacctgccg cgtctggcga tcggggccga agtgggcgcc aaagcgggcg ccgagcccgc
     4261 ggccgccagc accgacgcgc tcgccctgcc cccgcccgag gtcgcggtcg agcgcaaggc
     4321 caccgacccg ctgcacaagc tcaaggcctt cctcgacggc ttcgacggtc gcgtgctgct
     4381 gctctcggac tcgcccggcc ggcgcgagac catggccgag ttcttcgccg agtacgacgt
     4441 gcgccccgag gccacggccg atttcggcgc cttcctggat tccgacgccc gcctcgcgct
     4501 tggcgtcgcc ccgctcgcgc gcggcttcgt tctaccggct gcaaggctcg cggtgctgac
     4561 cgagaccgag ctctatgcct ccaccgcgcg cagccgcgtg cgccgcgacg cgcgcaaggc
     4621 cgccaccatg gaaggctggc tgcgcgacct ttccgagctc aagatcggcg acccggtggt
     4681 gcacgtctcg cacggcatcg gccgctacct cggcctcctc cacatgaacc tcggcgaggg
     4741 cgacaccgag ttcctgcacc tggaatacaa cggcggcgac aagctctatg tgccggtgtc
     4801 gcagctgcac gtgatcaccc gctatgcggg cgccgacccg gacgcgatcg acctgcaccg
     4861 cctcggctcc gggcagtggg agaaggccaa gaagaaggcc gcgatgcagg tgcgcgacac
     4921 cgccgccgaa ctgctcgcgc tctacgccca gcgcgccgcg cgtcccggcc accgcttcga
     4981 cttcaagcag catgacctcg aagccttcgc cgaggccttc ggcttcgaga ccacgcccga
     5041 ccagcaggcc gccatcgacg cggtggtggc cgacatgaag tccggccgcc cgatggaccg
     5101 cctggtgtgc ggcgacgtcg gcttcggcaa gaccgaggtc gcgc
//
LOCUS       DAOQNN010000226         5140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9756_length_5140_cov_8.005310, whole genome shotgun sequence.
ACCESSION   DAOQNN010000226 DAOQNN010000000
VERSION     DAOQNN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9756_length_5140_cov_8.005310"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..2518
                     /gene="xdhB"
                     /locus_tag="PKV97_11505"
     CDS             95..2518
                     /gene="xdhB"
                     /locus_tag="PKV97_11505"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HNB06548.1"
                     /translation="MNEPIEPTSSPPASAPPAGQPHPHESAHLHVAGEATYVDDLPEP
                     AGTAHAALGLSTQAHARIVAMDFGAVLAAPGVLGVLVAADIPGRNDCGPIVHDDPILA
                     AGEVHYVGQPIFAVIADEREGARRAARRARIDYAPLPARLDPLEAACAGELVLPPMRL
                     MRGDVDAALAAAPHRVEGRWQVGGQEHFYLEGQVALAQPREDGCLQVWCSTQHPSEMQ
                     QVVAHCLGIAAHRVVVEMRRMGGGFGGKESQSALFACVAALAAARFRRAVKLRPDRDD
                     DIAITGKRHDFHYDYTVGHDEEGRLLALRVDMVARAGCSADLSGPVATRALCHVDNAY
                     HLPAADLRARLARTHTQSNTAFRGFGGPQGAILAEWVIDGIARTLGRDALDVRRANFY
                     GGPGRDTTPYGQVVADNVLDALVDELEASSDYRARRAEIAAFNAAAPVLRRGLALTPV
                     KFGISFNLTHLNQAGALVHVYADGSVLVNHGGTEMGQGLHTKVCQVVAAELGIALQRV
                     RATATDTSKVANTSATAASTGADLNGQAAAAAARTIRMRLVEFALRRFAEGGEGGQGE
                     DRGDARPGRRPADPAAVRFADGQVYVGAHTIPFAELVGAAYRARVQLWSDGFYATPGL
                     HWDAATLTGHPFHYFAYGAAVSEVVVDTLTGEWRLLRADLLHDAGSSINPAIDRGQVE
                     GAFIQGMGWLTMEELWWNAEGRLMTHAPSTYKIPAAHDCPADLRVRLFHNPNRADTLL
                     RSKAVGEPPLLLGFSVFFALRDAIAAAGDGHSNPPLRAPATPEAILDALDAVRRPPTP
                     DASHLTPDA"
     gene            2554..>2899
                     /locus_tag="PKV97_11510"
     CDS             2554..>2899
                     /locus_tag="PKV97_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNB06549.1"
                     /translation="MPSWISTLSCLLETDARAVLVTVAATRGSAPRAAGAHMVVTAAQ
                     VQGSVGGGRLEQRAIGIARELLVTDAPSPRLERFALGASLGQCCGGVVVLAFERVGPA
                     QREWVGRAATLAA"
     assembly_gap    2900..2999
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3000..3626
                     /gene="xdhC"
                     /locus_tag="PKV97_11515"
     CDS             <3000..3626
                     /gene="xdhC"
                     /locus_tag="PKV97_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HNB06550.1"
                     /translation="SRDRADGSATAPGSAAALGTAILLDASPPPALQVVMFGAGHVGR
                     ALAELFGRLPLRVRWVDARAQEFPPALAPNVEPHCTDTPTAEVRAAPAGAVFLVLTHS
                     HALDFELVEAILARDDFRFCGLIGSQTKRARFAHRLHARGIEEARIARLRCPVGLPGL
                     PGKEAEVIALAIAAEVMRLRPQAHPPSTPRRPAAGAPATQPTESGHET"
     gene            3623..4930
                     /gene="guaD"
                     /locus_tag="PKV97_11520"
     CDS             3623..4930
                     /gene="guaD"
                     /locus_tag="PKV97_11520"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HNB06551.1"
                     /translation="MKPSAPPSPRLSAVRGEIVHFLADPAADPRALEHFADGVLIVRD
                     GHVAECGPAAALLPKLPAGTPLADHRGKLILPGFVDTHVHYPQTDIIASHGEQLLEWL
                     EKYTFPAERRFADRAHAAEVAGFFCDELLRNGTTTAAAFATVHAASVDALFEAARARR
                     MRMITGKVLMDRNCPDFLRDTAETGYAESKALIERWHNRDRLLYAITPRFAPTSTPAQ
                     MTLAGRLFNELPGVFLQSHLAENRAEVAWVAQLYPQARSYLDVYARAGQLGTRAVFAH
                     CIWLDDADRRHMAETGAAMSFCPTSNLFLGSGLFDLRRARTLGVRVGLGTDVGGGTSF
                     SMLQTMNEAYKVLQLGGQSLSAASAFHLATLGGAHSLYLDDRIGNLAPGKEADFVVLD
                     PRATPLLERRSAACATLEERLFVLMMLGDDRAVAATHLMGVPA"
     gene            complement(4950..>5140)
                     /locus_tag="PKV97_11525"
     CDS             complement(4950..>5140)
                     /locus_tag="PKV97_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB06552.1"
                     /translation="SEAIVLAILSLSHALGLEVVAEGVERPEQRDFLRHHGCDCFQGY
                     LFGKPLPMEAWGDFLEMI"
BASE COUNT          672 a         2028 c         1682 g          658 t
ORIGIN      
        1 ctcgagaccc gccccgacgc gccgctcgcc gcggcgcaga cgcgcgcctt cgccgcggaa
       61 gatctcgccg gcggcgggcg ctgaggagaa cgcgatgaac gagccgatcg agcccacctc
      121 gtcaccgccg gcatccgcac cccccgccgg ccagccgcat ccgcacgagt ccgcccacct
      181 gcacgtggcc ggcgaggcca cctacgtcga cgacctcccc gaacccgccg gcaccgcaca
      241 cgcggcgctc ggcctgtcga cgcaggcaca cgcgcgcatc gtggcgatgg acttcggcgc
      301 ggtgctcgcg gcgcccggcg tgctcggcgt gctggtggcc gccgacattc cgggacgcaa
      361 cgactgcggg ccgatcgtgc acgacgaccc catcctcgcc gcaggcgaag ttcactacgt
      421 cggccagccg atcttcgcgg tgatcgccga cgagcgcgag ggcgcgcgcc gcgccgcgcg
      481 ccgcgcccgc atcgactacg caccgctgcc ggcgcggctc gacccgctgg aggccgcatg
      541 cgcgggcgag ctcgtgctgc cgccgatgcg cctcatgcgt ggcgacgtcg atgcggcgct
      601 ggccgcggcg ccgcatcgcg tggaggggcg atggcaggtc ggcggccagg agcacttcta
      661 cctcgaaggc caggtcgcgc tcgcccagcc gcgcgaggac ggctgcctgc aggtgtggtg
      721 ttccacccag cacccgagcg agatgcagca ggtggtggcg cactgcctgg gcatcgccgc
      781 ccaccgcgtg gtcgtggaga tgcgccgcat gggcggcggc ttcggcggca aggagtcgca
      841 gtcggcgctg ttcgcctgcg tggcggcgct ggcggcggcg agatttcgcc gcgcggtcaa
      901 gctgcgcccc gatcgcgacg acgacatcgc catcaccggc aagcgccacg acttccacta
      961 cgactacacc gtcggtcacg acgaagaggg ccgcctcctc gcgctgcgcg tcgacatggt
     1021 cgcacgcgcg ggctgctccg ccgacctcag cggcccggtc gccacgcgcg cgctctgcca
     1081 tgtcgacaac gcctaccacc tgccggcggc cgacctccgt gcccggctcg cgcgcaccca
     1141 cacccagtcc aacaccgcct tccgcggttt cggcggaccg cagggcgcga tcctcgccga
     1201 gtgggtgatc gacggcatcg cgcgcacgct cgggcgcgat gccctcgacg tgcgccgcgc
     1261 caacttctac ggcggcccgg ggcgcgacac cacgccctac ggccaggtgg tcgccgacaa
     1321 cgtgctggac gcgctggtcg acgaactcga ggcgagcagc gactaccgcg cccgccgcgc
     1381 cgagatcgcc gccttcaacg ccgccgctcc ggtgctgcgg cgcgggctgg cgctcacccc
     1441 ggtgaagttc ggcatctcct tcaacctcac ccacttgaac caggccggcg cgctggtgca
     1501 tgtgtatgcc gacggctcgg tgctggtaaa ccacggtggt accgagatgg gccagggcct
     1561 gcacaccaag gtctgccagg tggtcgccgc cgagctcggc atcgcgctcc agcgcgtgcg
     1621 cgccaccgcc accgacacca gcaaggtggc caacacctcg gccaccgcgg cctccaccgg
     1681 cgccgacctc aacggccagg ccgcggccgc cgccgcgcgc acgatccgca tgcgcctggt
     1741 ggagttcgca ctgcgccgct tcgccgaggg gggcgagggg ggccaaggcg aagaccgcgg
     1801 cgatgcgcgc ccaggccgcc ggcccgccga cccggccgcg gtgcgcttcg ccgacggcca
     1861 ggtgtacgtc ggtgcacaca cgatcccctt cgccgagctc gtcggcgcgg cctaccgcgc
     1921 ccgcgtgcag ctgtggtccg acggcttcta cgccacgccc ggactgcact gggacgcggc
     1981 cacgctcacc ggccacccct tccactactt cgcctacggc gcggcggtgt ccgaggtggt
     2041 ggtcgacacg ctcaccggcg aatggcgcct gctgcgcgcc gacctgctgc acgacgccgg
     2101 cagctcgatc aacccggcga tcgaccgcgg ccaggtcgag ggcgccttca tccagggcat
     2161 gggctggctc accatggaag agctgtggtg gaacgccgag ggccgcctga tgacccacgc
     2221 gccttccacc tacaagatcc ccgccgcgca cgactgcccg gcggacctgc gcgtgcgcct
     2281 gttccacaac cccaaccgcg ccgacaccct cctgcgctcc aaggcggtcg gcgaaccgcc
     2341 gctgctgctc ggtttctcgg tcttcttcgc gctccgcgac gccatcgccg ccgccggcga
     2401 cggccacagc aacccgcccc tgcgcgcacc cgccacccct gaggccatcc tcgacgcgct
     2461 cgacgcggtg cgccggcccc cgacgcccga cgcctcccat ctgacgcccg acgcctgaca
     2521 cccgacgttt cgcccttcgc agctcacctc cccttgccct cctggatctc caccctctcc
     2581 tgtctgctcg agaccgacgc ccgcgcggtc ctggtcacgg tcgccgccac acgaggttcg
     2641 gcaccgcgcg ccgcgggtgc gcacatggtg gtgacggccg cccaggtgca gggcagcgtc
     2701 ggcggcggcc ggctggagca gcgcgccatc gggatcgcgc gcgagctgct cgtcaccgac
     2761 gccccttccc cgcgcctcga acgtttcgcc ctcggcgcca gcctcggcca gtgctgcggc
     2821 ggcgtcgtgg tgctggcctt cgagcgcgtc ggcccggcac agcgggagtg ggtcgggcgc
     2881 gccgccacgc tcgccgccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     3001 gtcgcgatcg ggccgacgga tcggcgacag cgcccggatc cgccgcggcg ctcgggacgg
     3061 cgatcctgct cgatgcgagc ccgccgccgg cgctgcaggt ggtgatgttc ggcgcgggcc
     3121 atgtggggcg ggcgctggcg gagctgttcg ggcggctgcc gctgcgcgtg cgctgggtcg
     3181 acgcgcgcgc gcaggaattc ccgcccgcgc ttgcgcccaa tgtcgaaccg cactgcaccg
     3241 acacgccgac ggcggaggtg cgcgcggcgc ccgcgggcgc ggtcttcctg gtcctcaccc
     3301 acagccacgc gctcgacttc gagctggtcg aagccatcct cgcgcgcgac gacttccgct
     3361 tttgcggcct gatcggctcg cagaccaagc gcgcgcgctt cgcccaccgc ctgcacgcgc
     3421 gcggaatcga agaagcgcgc atcgcacgcc tgcgctgccc ggtcggcctg cccggcctgc
     3481 cgggcaagga agccgaggtg atcgcgctcg cgatcgccgc cgaagtgatg cgcctgcgcc
     3541 cgcaagcgca tcccccgagc acgccgcgcc ggcccgcagc gggcgcaccg gccacgcaac
     3601 ccacggaatc cggccacgag acatgaagcc ctccgcccca ccctccccgc ggctctccgc
     3661 cgtacgcggc gagatcgtcc acttcctcgc cgaccccgcg gccgacccgc gcgcgctcga
     3721 gcacttcgcc gacggcgtgc tgatcgtgcg cgacggccac gtcgccgaat gcggccccgc
     3781 cgccgcgctg ttgcccaagc tgcccgccgg cacgccgctc gccgaccacc gcggcaagct
     3841 gatcctgccc ggcttcgtcg acacccacgt gcattacccg cagaccgaca tcatcgccag
     3901 ccacggcgag cagctgctcg aatggctgga gaaatacacc ttccccgccg agcgccgctt
     3961 cgccgaccgc gcgcatgccg ccgaggtcgc cggcttcttc tgcgacgagc tgctgcgcaa
     4021 cggcaccacc accgcggcgg ccttcgccac ggtgcacgcg gcctcggtgg atgcgctctt
     4081 cgaggccgcg cgcgcgcgcc gcatgcgcat gatcaccggc aaggtgctga tggaccgcaa
     4141 ctgccccgac ttcctgcgcg acacggcgga gaccggctac gcggagtcga aggccttgat
     4201 cgagcgctgg cacaaccgcg accgcctgct gtacgccatc accccgcgct tcgcgcccac
     4261 ctccacgccc gcgcagatga cgctcgccgg ccgcctcttc aacgagctcc cgggcgtctt
     4321 cctgcagtcg cacctcgccg agaaccgcgc cgaggtggcc tgggtggcgc agctgtaccc
     4381 gcaggcgcgc agctacctcg acgtgtatgc ccgcgccggc cagctcggca cgcgcgcggt
     4441 gttcgcgcac tgcatctggc tcgacgacgc cgaccgccgg cacatggccg agaccggcgc
     4501 ggcgatgagc ttctgcccca cctccaacct cttcctcggc tcggggctgt tcgacctgcg
     4561 gcgcgcgcgc acgctcggcg tgcgcgtggg actgggcacg gacgtggggg gcggcaccag
     4621 cttctcgatg ctgcagacca tgaacgaggc ctacaaggtg ctgcagctcg gcggccagtc
     4681 gctgtcggcg gcgagcgctt tccacctcgc caccctgggc ggcgcgcaca gcctctacct
     4741 cgacgaccgc atcggcaacc tcgcgcccgg caaggaggcc gacttcgtcg tcctcgaccc
     4801 gcgcgccacg cccctgctcg agcgccgcag cgccgcctgc gcgacgttgg aagagcgcct
     4861 cttcgtgctg atgatgctcg gcgacgaccg cgcggtggcg gccacgcact tgatgggcgt
     4921 gccggcctga ggcccgcgcg cgaccggcct cagatcatct cgaggaagtc gccccaggcc
     4981 tccatcggca gcggcttgcc gaacaggtag ccctggaagc agtcgcatcc gtggtgacgc
     5041 aggaagtcgc gctgctccgg cctctccact ccttcggcca ccacctccag gccgagcgcg
     5101 tggctcagcg acaggatcgc cagcacgatc gcctcgctgc
//
LOCUS       DAOQNN010000227         5130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9785_length_5130_cov_10.925517, whole genome shotgun sequence.
ACCESSION   DAOQNN010000227 DAOQNN010000000
VERSION     DAOQNN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9785_length_5130_cov_10.925517"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..1651
                     /locus_tag="PKV97_11530"
     CDS             11..1651
                     /locus_tag="PKV97_11530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNB06553.1"
                     /translation="MIPPGGTAGAPAAAASVGAHGAIARGLALAVLALAGVFVLLTFD
                     QHGISNDEEVQHVYGRLLLDFYASSFADRQAFEYKNLYLYGGFFDLLAAAFERAGVAE
                     GPALWDLRHLISAVFGLLGLAGTWLLARRLAGEWAGLAALVLLSITGSWSGAMFTHTK
                     DIPFATTMLWALYFSVRVLDTLPAPPWRVLAGLGVALGCAFGLRIGAVFAVFYLGVGV
                     LAATALQPGGRVRFLLRGVLALLPAAAIALALGALFWPWAAMEPGNVLTAMRAFSHFS
                     FELDTVLAGRVMNVGEVPGHYLAAYLLVRLPELFLAGLALALLLGVRAVPALAGEQAL
                     RAALPWLPVVLAALFPLVYTLLAAPPLYNGLRHFSFVLPPLAVLAGMGLVRAWHGLSV
                     RPPLLRRAVLGACALAVLGQLGQLARLHPYEYLAYNRLAGGVQGAVGRWEQDYWASSL
                     REAVHALNALVAREGGAGRHYSVAVCAEPLQAQVWLAPGLRATRDWWGADFYLSPTHM
                     GCDEAMRGRVVAQVERAGLVLAVVKDRRALVGEERRPR"
     gene            1648..2058
                     /locus_tag="PKV97_11535"
     CDS             1648..2058
                     /locus_tag="PKV97_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNB06554.1"
                     /translation="MSPSGGCARFLRFAAVGAVGTLAHYALLLALVEGADAPPLVGAI
                     AGFMLGALVNYALARRLVFASTRSHAQALPRFFAVALAGLAWTALLMSLFMELLGLHY
                     LLAQVLTTALLLLWHYAGNALWTFRRGRRDTESR"
     gene            2055..2960
                     /locus_tag="PKV97_11540"
     CDS             2055..2960
                     /locus_tag="PKV97_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB06555.1"
                     /translation="MSAQAPAGTPPHRLEADLLLVLTTLIAAAGWIFSKEALTGMPPL
                     IFIGLRFAIAGLVLALFSLPQLRALDRRGLRNSLLVGALFAGGMVLWILGLSHSSHLG
                     EASFISSMGIVMVPVFARLFFGDRPPASTWFALPLALAGFACLSLDGGFEVELGQWFF
                     LGAALVFALMFNVNSHIVRNVPVRALSVVQMLAVGVLVLPPALLIETWPQAWSAPVLG
                     WLLASALLATTLRFLLQLHGQSLTTPSHAALIMMLEPVWTALAAAWWFAETMSTLQLA
                     GCGLIFLALVVSRWAWIRGLFGGRG"
     gene            2982..3506
                     /gene="def"
                     /locus_tag="PKV97_11545"
     CDS             2982..3506
                     /gene="def"
                     /locus_tag="PKV97_11545"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNB06556.1"
                     /translation="MAVRTLLRMGDARLLQPAAPVGEFGTPALAALIEDLFDTMAAHG
                     GVGLAAPQIGVGLQVVIFGFERSERYPDAAPVPRTILLNPVIVPLTEEREEGWEGCLS
                     VPGLRGMVPRATRIRYTGCTPAGEPIERFAEGFHARVVQHECDHLAGVLYPMRVRDFR
                     RFGFTDVLFPELQG"
     gene            3596..4939
                     /locus_tag="PKV97_11550"
     CDS             3596..4939
                     /locus_tag="PKV97_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB06557.1"
                     /translation="MRLKTCLGCGVVVLASLWLSLAHAADKAVLFENVFVFDGRSSER
                     GRSPVNVLVVGDTIQTISAAPIAPPADAELTRVAGGGRTLMPGLIDAHWHAMLIGQKL
                     VDAMTSDVGYTNLRAAQVAEATLMRGFTTVRDMGGPVQGLRRAIEEGRFPGPRIFPSG
                     AMISQTGGHGDFRLPHEVPRAASQGLSHTELTRTAAIADGADEVLRRTREQLMLGATQ
                     IKLMAGGGVTSIYDPIDATQYTQDEIRAAVAAAENWGTYVTVHAYTSRAVQVAIEAGV
                     KAVEHGQLVDEETVRLMAQKGIWWSLQPFLDNELANPQAGANRVKQLMVAAGTDRAYA
                     LARKHAVKVAFGTDILFSGDNGEVQNARLVSLERWYPPGEVLQIATGNNGALLELTGE
                     RNPYRKPLGVVAEGALADLLLVDGDPTADLSLIKRPESSFVLIMKNGRIYKNLLP"
BASE COUNT          604 a         1784 c         1856 g          886 t
ORIGIN      
        1 agcgcgatcg atgatcccgc caggcgggac ggcgggtgcg ccggccgcgg cggcatccgt
       61 cggggcgcat ggcgcgatcg cgcgcgggct cgccctcgcc gtgctggcgc tggccggggt
      121 gttcgtgctg ctcaccttcg accagcacgg catcagcaac gacgaggagg tccagcatgt
      181 ctacggccgc ctgctgctcg acttctacgc ctccagcttc gccgaccgcc aggccttcga
      241 atacaagaac ctctatcttt acgggggctt cttcgacctc ctcgcggcgg ccttcgagcg
      301 cgccggggtg gccgaagggc cggcgctgtg ggacctgcgc cacctgatct cggccgtctt
      361 cggtctcctc gggctggccg gcacctggct gctcgcgcgc cggctcgcgg gcgagtgggc
      421 ggggctggcg gcgctcgtgc tgttgtcgat caccggctcg tggtcgggcg cgatgttcac
      481 ccacaccaag gacatcccct tcgccaccac gatgctgtgg gcgctgtact tcagcgtgcg
      541 cgtgctcgac acgctgcccg cgccgccgtg gcgcgtgctc gcggggctgg gtgtggcgct
      601 cggctgcgcc ttcggcctgc gcatcggtgc ggtgttcgcg gtgttctacc ttggcgtcgg
      661 cgtgctcgcg gcgacggcct tgcagccggg cgggcgggtg cgtttcctgc ttcgcggcgt
      721 gctggcgctg ctgccggcgg cggcgatcgc gctggcgctg ggcgcgctgt tctggccgtg
      781 ggcggcgatg gagccgggta acgtgctcac ggcgatgcgc gcgttctcgc atttcagctt
      841 cgagctcgac accgtgctgg ccgggcgcgt gatgaacgtc ggcgaggtcc cggggcatta
      901 cctcgcggcc tacctgctgg tgcgcctgcc ggagcttttc ctcgccgggc tcgcgctcgc
      961 gctgctgctc ggcgtgcgcg ccgtgccggc gctcgctggc gagcaggccc tgcgcgcggc
     1021 cctgccgtgg ctgccggtgg tgctggcagc gctgttcccg ctcgtctaca ccctgcttgc
     1081 ggcgccgccg ctgtacaacg ggctgcgcca tttcagcttc gtgctgccgc cactggcggt
     1141 gctggcgggc atggggctcg tgcgcgcatg gcacggcctg agcgtccgtc cgccgctgct
     1201 gcgccgcgcc gtgctgggcg cgtgcgccct ggcggtcctc ggccaactcg gccagctcgc
     1261 ccgcctgcat ccctacgagt atctcgccta caaccgcctc gcgggcggcg tgcagggggc
     1321 ggtcgggcgc tgggagcagg actactgggc gagcagcctg cgcgaggcgg tgcacgccct
     1381 caacgccctg gtcgcgcgcg aggggggggc agggcgacac tattccgtgg ccgtgtgcgc
     1441 cgagccgctg caagcccagg tgtggctcgc gcccgggttg cgcgcgacgc gcgactggtg
     1501 gggggcggac ttctacctct cccccaccca catgggttgc gacgaggcca tgcggggccg
     1561 cgtggtggcg caggtcgagc gtgcggggct ggtgctggcg gtggtcaagg atcggcgcgc
     1621 gctggttggc gaggagcggc ggccccgatg agcccgtccg gcggatgcgc gcgcttcctg
     1681 cgctttgccg cggtgggggc ggtgggtacg ctcgcccact acgcgctgtt gctcgcgctg
     1741 gtggagggcg cggatgcgcc gccgctggtg ggggccatcg ccggattcat gctcggcgcg
     1801 ctggtcaact acgccctcgc gcgccggctg gtgttcgcgt ccacgcgcag ccacgcccag
     1861 gccctgccgc gcttcttcgc cgtggcgctg gccgggctgg cgtggacggc gctgctgatg
     1921 agtctcttca tggagctgct cggcctgcac tacctgctcg cccaggtgtt gacgaccgcg
     1981 ctgctgctgc tgtggcacta cgccggcaac gccctgtgga ccttccgccg cggccggcgc
     2041 gacacggaga gccgatgagc gcacaggctc ccgccggtac gccgccgcat cgcctcgagg
     2101 ccgacctcct gctcgtgctc accacgttga tcgccgcggc gggctggatc ttctccaagg
     2161 aggcgctcac cggcatgccg ccgctgatct tcatcggcct gcgcttcgcc atcgccgggc
     2221 tggtgctggc actgttctcg ctgccgcagc tgcgcgcgct cgatcggcgc ggactgcgca
     2281 acagcctgct cgtcggcgcg ctgttcgccg gcggcatggt gctgtggatc ctcggcctgt
     2341 cgcacagcag ccacctcggc gaagcctcgt tcatctccag catgggcatc gtcatggtgc
     2401 cggtgttcgc gcgcctgttc ttcggcgacc gtccgccggc gagcacctgg ttcgcgctgc
     2461 cgctcgcact cgccggcttc gcctgcctgt cgctcgacgg cggcttcgag gtcgagctcg
     2521 ggcagtggtt cttcctcggc gcggcgctgg tgttcgcgct catgttcaac gtcaattcgc
     2581 acatcgtgcg caacgtgccg gtgcgcgcgc tcagcgtggt gcagatgctc gccgtcggcg
     2641 tgctggtgct gccgccggcc ttgctgatcg agacctggcc gcaggcctgg agcgcgccgg
     2701 tgctcggctg gctgctcgcg agcgcgctgc tcgccaccac gctgcgcttc ctgctccagc
     2761 tccacggcca gagcctgacc acgcccagcc atgcggcgct gatcatgatg ctcgagccgg
     2821 tgtggaccgc gctcgccgcg gcgtggtggt tcgccgagac catgagcacg ctgcaactcg
     2881 ccggctgcgg gctgatcttc cttgccctcg tggtgagccg ctgggcgtgg atccgcggcc
     2941 tcttcggcgg gcgcggctga acgatcgggg agagagattc catggctgtc cgtacccttc
     3001 tgcgcatggg tgatgcgcgc cttctgcagc cggccgcgcc ggtcggcgaa ttcggcacgc
     3061 ctgcgctcgc cgcgctcatc gaggacctgt tcgacaccat ggccgcgcac ggcggtgtgg
     3121 gcctggccgc gccgcagatc ggcgtcggcc tgcaggtggt gatcttcggc ttcgagcgca
     3181 gcgagcgcta cccggacgcc gcgccggtgc cgcgcaccat cctgctgaac ccggtgatcg
     3241 ttccgctcac ggaagagcgc gaggagggct gggagggctg cctgtcggtg cccggcctgc
     3301 gcggcatggt gccgcgcgcc acgcgcatcc gctacaccgg ctgcacgccc gcgggcgagc
     3361 cgatcgagcg cttcgccgag ggcttccatg cgcgcgtggt gcagcacgag tgcgaccacc
     3421 tcgccggcgt gctgtatccg atgcgggtgc gggatttccg ccgcttcggg tttacggacg
     3481 tgctgtttcc ggagctgcag ggatagccaa ggcctggctt tatccccagg acgggggtgg
     3541 cggcccggac tgctatcctg atgattgttt cgatcacgtc cagaggggtc gtccgatgag
     3601 gctgaaaacc tgtctcggtt gcggggtcgt cgtgctcgca tcgttgtggc tgtcgctggc
     3661 gcatgcggcg gacaaggccg tcctgttcga gaatgtcttc gtcttcgatg gcaggagcag
     3721 cgagcggggc cgctcgccgg tcaacgtgct ggtggtgggc gacaccatcc agaccatctc
     3781 ggcggcgccg atcgcgccgc ccgcggacgc cgagctgacg cgtgtcgccg gcgggggccg
     3841 cacgctgatg cccggcctga tcgatgcgca ctggcacgcg atgctgatcg gccagaagct
     3901 ggtcgatgcc atgaccagcg acgtcggcta taccaatctg cgtgcggcgc aggtcgccga
     3961 ggcgacgctg atgcgcggct tcaccacggt gcgcgacatg ggcgggccgg tgcagggcct
     4021 gcggcgtgcg atcgaggagg ggcgtttccc cgggccgcgc atcttcccgt cgggcgcgat
     4081 gatctcgcag accggcggcc atggcgattt ccgtctgccg cacgaggtgc cgcgcgccgc
     4141 cagccagggt ctcagtcata ccgaacttac ccgcaccgcc gccattgccg atggcgccga
     4201 cgaggtgctg cggcgcaccc gcgaacagct gatgctcggc gcgacgcaga tcaagctgat
     4261 ggctggcggt ggggtgacgt cgatctacga cccgatcgac gccacccaat acacccagga
     4321 cgagatccgc gccgcagtgg ctgcggcaga gaactggggc acttacgtta cggtgcacgc
     4381 ctacaccagc cgtgcggttc aggtcgccat cgaggccggg gtcaaggcgg tggagcacgg
     4441 ccagctggtc gatgaggaga cggtcaggct gatggcgcag aaggggatct ggtggtcgct
     4501 ccagcccttc ctcgacaacg agctcgccaa cccgcaggcc ggggccaacc gggtcaagca
     4561 actgatggtg gcggccggca ccgatcgcgc ctacgcgctg gcgcgcaagc acgcggtgaa
     4621 ggtggccttc ggcaccgaca tcctgttctc gggggacaac ggagaggtgc agaacgcacg
     4681 cctggtctcg ctcgagcgct ggtatccccc cggcgaggtg ctgcagatcg cgaccggcaa
     4741 caacggcgcc ctgctcgagc ttaccggcga gcgcaacccg tatcgcaagc cgctcggggt
     4801 cgtcgccgag ggcgcgctcg ccgacctgtt gctggtcgat ggcgacccga cggcggacct
     4861 gtcgctcatc aagcgtcccg agtcgagctt cgtcctcatc atgaagaacg ggcgcatcta
     4921 caagaacctg ctgccctgag cggcgccttc gcagcccccc atgcgttcga gtttgctcat
     4981 ccgtgccggc atggtggcgc cggtcaccat tgcgctgccg ccgacggcgg gcgcacagcc
     5041 accagcctcc gccgacttcc tcgacgccga agggggcgcc ggtcagacgt tgaacaggaa
     5101 gttcagcacg tcgccgtcct tgaccacgta
//
LOCUS       DAOQNN010000228         5113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9835_length_5113_cov_9.763345, whole genome shotgun sequence.
ACCESSION   DAOQNN010000228 DAOQNN010000000
VERSION     DAOQNN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9835_length_5113_cov_9.763345"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PKV97_11555"
     CDS             <1..343
                     /locus_tag="PKV97_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HNB06558.1"
                     /translation="RCAGVCARRETPEEHDARLAGALAGVGVKPWPWAGPVAVEEVRA
                     HGQHRLQVFDRWCHLGSAQDEAGALALAAAPRAFDADVWRILARWLAVPAHLAQVRVL
                     AAQAGEGERGG"
     gene            complement(397..1098)
                     /locus_tag="PKV97_11560"
     CDS             complement(397..1098)
                     /locus_tag="PKV97_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB06559.1"
                     /translation="MSCRPPSLAPDASDLLARFAPFDMLDPCARERVLAAARPIHAVR
                     GELLVQQEDCPRGIYLVLDGRVKCFVLSPSGGEKIVRLAEPGSFFGEEAALINRPHVA
                     NAQAIGTSELLLVPTAALRTAMVSSTAFSVSMSNRLAEASYDLMSTMQVQFQLSGTQR
                     VAHYLTRLAPEDAERCEIRLDIDKQTIAAQLNLTPETLSRILSQLTRDGMIQPRGRRG
                     MVLSKLSQLRDCAAH"
     gene            1355..3268
                     /locus_tag="PKV97_11565"
     CDS             1355..3268
                     /locus_tag="PKV97_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB06560.1"
                     /translation="MYLAVPRLLVRRSIMLLVSVALVAMTLIGLAGMSASFVVVDRSR
                     DSVQAIAVASTLRGHTQRIANLIAIDALKGRIGVSERTRAAMADIERELQQAALRRFV
                     DEAPGELFAATYRGVQAGWERSVRPRIEALAGPVPPDAREVETLLAGVDDFVGQVDSL
                     VAVLGQENERRIDELRRILALAAAVTLGVVLVVIVLLQRALLRPLGGLLDAARRIAGG
                     DFGVRVRYTGEDELGQVGSAFNLMADELARHYHLLELRVTEKTAELQRSNRSLELLYH
                     AIARLYQAPSAPDAYEATLRDIDRVAGLEGSFVCIEPRPGAPAAVIASSMGPCPDRAE
                     RGEDACAACRAGSEGQALALLSPTLLRFPLRDREHHHGMLRLSLSDGARLEDWQRQLV
                     EALSRHIGMALGAARRTEQERLLALQEERSVIARELHDSLAQALSYMKIQVSLLQRAL
                     ADPARTAEAEPILADLREGISAAYRQLRELLVSFRLGLSADLATLMEDAAREYGTRGG
                     LEVELAVELGACQLSPNQEVHVLQIVREALSNMVRHASARHAWVALRGGADGEVLLEV
                     RDDGCGIGAPPADARNHHGLAIMRERARSMGGEIDIGPALPSGTRVCVRFRSANASMA
                     AMAQGDAKKETET"
     gene            3265..3909
                     /gene="narL"
                     /locus_tag="PKV97_11570"
     CDS             3265..3909
                     /gene="narL"
                     /locus_tag="PKV97_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HNB06561.1"
                     /translation="MNESCRVLIVDDHPLFRRGLVQLLRTIPAFVLVGEAASGAEGLV
                     LARELRPDLVLLDLNMRDGDGLDMLRGLRAARSEARVVMVTVSDSGEDVVAALRGGAE
                     GYLLKDMEPEAMLEALQAVAAGRVVIPPQLNHLLAAALRGESRPQSAGAAGLTEQELR
                     ILEKIAAGLPNKQIGRELDIAEGTVKVHVKHILRKLELRSRVEAAVWAVEHLRA"
     gene            4035..4571
                     /gene="napF"
                     /locus_tag="PKV97_11575"
     CDS             4035..4571
                     /gene="napF"
                     /locus_tag="PKV97_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HNB06562.1"
                     /translation="MASSASPRAASSRRNFLFGRTRAVEPALRPPWALEEDDFVARCT
                     RCERCIEACPTTILVRMDGGFPGVDFSRGECSFCGDCVAACEPRALWREDASAAPWDR
                     VAVIGAACLAARGVECRVCGEACPEGAIRFRPRLGGVAHPELDAAACTACGACIGPCP
                     TRAIDTRVRNPLPTESAT"
     gene            4568..4822
                     /locus_tag="PKV97_11580"
     CDS             4568..4822
                     /locus_tag="PKV97_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HNB06563.1"
                     /translation="MNIASLVVRAFPADFPEIVEGLKKVPGVELHGQSAEKGNIVITI
                     EDGAGWSVTDSILAVNLLPKVQGLTIAYEYTDEGLELTEV"
     gene            4824..>5113
                     /locus_tag="PKV97_11585"
     CDS             4824..>5113
                     /locus_tag="PKV97_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNB06564.1"
                     /translation="MSMNRREFIKNNAIAAAAATAGIGIPVVAEAQQPGAGGTKVRWD
                     KAACRFCGTGCSVLVGVQDGRVVATQGDPDSPVNRGLNCIKGYFLSKIMYGE"
BASE COUNT          705 a         1721 c         1910 g          777 t
ORIGIN      
        1 gcgctgcgcg ggcgtgtgcg cgcggcgcga gacccccgag gagcacgacg cccgcctcgc
       61 cggcgcgctc gccggggtgg gcgtcaagcc ctggccgtgg gcggggccgg tggcagtgga
      121 agaggtgcgc gcgcacggcc agcaccggct gcaggtgttc gaccgctggt gccacctggg
      181 cagcgcgcag gacgaggccg gcgcgctcgc cctcgccgcg gcgccgcgcg ccttcgacgc
      241 cgacgtctgg cgcatcctcg cgcgctggct ggcggtgccg gcccatctcg cgcaggtgcg
      301 cgtgctcgcc gcgcaggcgg gcgaagggga gcggggcggt taagcggcgc aggctgccgc
      361 ggtggtgccg gccacgaacg atgcgccgga cgcggcctag tgcgccgcgc agtcgcgcag
      421 ctgcgacagc ttgctgagca ccatgccgcg gcggccgcgc ggctggatca tgccgtcgcg
      481 ggtgagctgc gagaggatgc gcgacagcgt ttccggggtc aggttgagct gcgcggcgat
      541 ggtctgcttg tcgatgtcga ggcggatctc gcagcgctcg gcatcttccg gcgcgagccg
      601 ggtcaggtag tgggcgacgc gctgcgtgcc gctgagctgg aactgaacct gcatggtcga
      661 catcagatcg tagctggctt ccgcgagccg gttgctcatc gacaccgaaa aggcggtcga
      721 gctcaccatc gcggtgcgca gcgcggcggt cggcaccagc agcagctcgc tcgttccgat
      781 cgcctgggcg ttggccacgt gtgggcggtt gatcagcgcg gcctcctcgc cgaagaagct
      841 gccgggctcg gcgaggcgga cgatcttctc gccgcccgag ggcgagagga cgaagcattt
      901 gactcttccg tcgagcacca ggtagatacc gcgcggacag tcctcctgct gcacgaggag
      961 ttcgccgcgg acggcgtgga tcggccgcgc ggcggcgagc acgcgctcgc gggcgcaagg
     1021 gtcgagcatg tcgaagggag cgaagcgggc gagcaggtcg ctggcgtcgg gggcgaggga
     1081 ggggggcctg cagctcatgc ggatctcctg cggacgtcgg cgtggttttc cagtcggtag
     1141 actagccgct ggtttccacc ctgcatggcg tcgcgacctg cagcggggcg cgcgcgtgtt
     1201 tttggcatac ctccgaagag gtatccgcgg attttggcaa gtcactgaaa agtagacgca
     1261 aaggtcgagg cttgcaggcg ttgcagccga ggatcgaagt ggaaggggta tgtccacgtt
     1321 gttgtcccgg atcgcgtccg gatggagatt tcacatgtat ctggcggtcc cccgcctgct
     1381 cgtgaggcgc tcgatcatgc tgctggtgtc ggtcgccctc gtggcgatga cgctgatcgg
     1441 gctggcgggc atgagcgcat ccttcgtcgt ggtggatcgc agccgcgaca gcgtgcaggc
     1501 gatcgccgtg gccagcaccc tgcgcggcca tacccagcgg atcgccaacc tgatcgcgat
     1561 cgatgcgctc aagggccgca tcggtgtgtc cgagcgcacg cgcgcggcga tggccgacat
     1621 cgagcgcgag ctgcagcagg ccgcgctgcg ccgcttcgtg gacgaggccc cgggagagct
     1681 cttcgcggcg acctaccgcg gcgtgcaggc gggttgggag cgctcggtgc ggccgcggat
     1741 cgaggcgctc gccgggcctg tgccgccgga tgcgcgcgag gtcgagacgc tgctcgccgg
     1801 ggtcgacgac ttcgtcggtc aggtcgatag cctggtcgcg gtgctcggcc aggagaacga
     1861 gcgccgtatc gacgagctgc gccgcatcct cgcgctcgcc gcggcggtga cgctgggggt
     1921 ggtgctggtg gtgatcgtct tgctgcagcg ggcgctgctg cgcccgctcg gtggcctgct
     1981 cgacgccgca cgccgcatcg ccggcggtga tttcggcgtg cgcgtgcgct acaccgggga
     2041 ggacgaactc ggccaggtcg gcagcgcctt caacctgatg gccgacgagc tcgcccgcca
     2101 ctatcacctg ctcgagctgc gcgtcaccga gaagaccgcc gagctgcagc gcagcaaccg
     2161 ttcgctcgag ctgctctacc acgccatcgc ccggctctac caggcgccga gcgcgcccga
     2221 cgcctacgag gccaccctgc gcgacatcga ccgcgtcgcc gggctggagg gctccttcgt
     2281 ctgcatcgag ccgcgccccg gcgcccccgc ggcggtgatc gcctcgtcca tggggccctg
     2341 tccggatcgc gccgagcgcg gcgaggacgc ctgcgcggcc tgccgggccg gctcggaggg
     2401 gcaggcgctg gcgctgctgt cgccgaccct gctgcgcttt ccgctgcgcg accgcgagca
     2461 ccatcacggc atgctgcgcc tgtcgctgtc cgacggcgcg cgcctggagg actggcagcg
     2521 ccagctcgtc gaggcgctgt cgcgccacat cggcatggcg ctcggcgcgg cacggcgcac
     2581 cgagcaggag cgcctgctcg cactgcagga ggagcgctcg gtgatcgcgc gcgagctgca
     2641 cgattcgctc gcgcaggcgc tgtcctacat gaagatccag gtcagcctgt tgcagcgcgc
     2701 actcgccgat ccggcgcgca cggcggaggc ggagccgatc ctggccgacc tgcgcgaggg
     2761 catcagcgcc gcctaccgtc agctgcgcga gctgctggtg tccttccgcc tcggcctgtc
     2821 ggccgacctc gccaccctga tggaagatgc ggcgcgcgaa tacggcacgc gcggcggcct
     2881 ggaggtcgag ctcgccgtgg agctcggcgc ctgccagctc agcccgaacc aggaggtgca
     2941 tgtgctgcag atcgtgcgcg aggccttgtc gaacatggtg cgccacgctt cggcacgcca
     3001 tgcctgggtg gcgctgcgtg gcggcgcgga tggcgaggtg ctgctggagg tgcgcgacga
     3061 cggctgcggc atcggcgcgc cgccggcgga tgcccgcaac caccacgggc tggcgatcat
     3121 gcgcgagcgc gcgcgcagca tgggcgggga aatcgacatc gggccggcgc tgccgagcgg
     3181 tacccgggtg tgcgtgcgct tccgatccgc caacgcgagc atggcggcga tggcgcaggg
     3241 tgatgcgaag aaggaaacgg agacatgaac gagagctgtc gcgtgctgat cgtggacgac
     3301 cacccgctgt tccgacgcgg actggtgcag ctgctgcgca cgattcccgc cttcgtcctc
     3361 gtcggcgagg ccgccagtgg cgcagaaggg ctcgtgctcg cgcgcgagct gcgccccgac
     3421 ctggtcctgc tcgacctcaa catgcgcgac ggcgacgggc tcgacatgct gcgcggcctg
     3481 cgcgcggcgc gcagcgaggc gcgcgtggtg atggtcacgg tgtcggactc cggcgaggat
     3541 gtggtggccg ccttgcgcgg cggcgccgag ggctacctgc tcaaggacat ggagcccgag
     3601 gccatgctcg aggcgctgca ggccgtggcc gccgggcggg tggtgatccc cccgcagctc
     3661 aaccacctcc tcgccgccgc gttgcgcggc gagtcgcggc cacaatcggc gggcgccgcc
     3721 gggctcaccg agcaggagct gcgcatcctc gagaagatcg ccgccgggct ccccaacaag
     3781 cagatcgggc gcgagctcga catcgcggag ggtacggtga aggtccacgt caagcacatc
     3841 ctgcgcaagc tcgagctgcg ctcgcgcgtc gaggccgcgg tgtgggcggt ggagcatctg
     3901 cgggcctgag cggcggggaa gggcgcgcgc ggcaagcccg gctgtattga gatgagttcg
     3961 caggttgact caggtcaata attgactgct gctactgggc tgtaatggcg gctgcgcaat
     4021 ttgctggatc gtccttggct tcctccgcat ctccgcgcgc ggcgagttcc cgccggaact
     4081 tcctcttcgg acgcacgcgc gcagtcgagc ccgcactccg cccgccctgg gcgctggagg
     4141 aggacgactt cgtcgcgcgt tgcacgcgct gcgagcgttg tatcgaggcc tgtcccacca
     4201 ccatcctggt gcgcatggac ggcggcttcc ccggggtcga tttcagccgc ggcgaatgca
     4261 gcttctgcgg cgattgcgtc gccgcctgcg aacctcgcgc gctttggcgc gaggacgcgt
     4321 ccgctgcgcc ctgggatcgt gtcgccgtga tcggcgcagc ctgcctggcg gcgcgcggcg
     4381 tcgaatgccg cgtgtgcggc gaggcctgcc ccgagggcgc catccgcttc cgtccccgcc
     4441 tcgggggcgt cgcccatccc gaactcgacg ccgccgcgtg caccgcctgc ggcgcctgca
     4501 tcggcccgtg cccgacgcgt gcgatcgaca cgcgcgtgcg caatcccctc cctacggaga
     4561 gcgcaacatg aatattgcaa gtctggtcgt gcgggccttt cccgcggatt tccccgagat
     4621 cgtcgagggc ctgaagaagg tgcccggggt cgagttgcac gggcagtccg ccgagaaggg
     4681 caacatcgtc atcaccatcg aggacggcgc gggctggtcg gtgaccgact cgatcctggc
     4741 ggtgaacctg ctgcccaagg tgcaggggct gacgatcgcc tacgaataca ccgatgaagg
     4801 tctggaattg acggaggtct gacatgagca tgaatcgacg cgaattcatc aagaacaacg
     4861 cgatcgccgc ggcggccgcc accgccggca tcgggatccc ggtcgtcgcc gaggcgcagc
     4921 agcccggcgc gggcggcacc aaggtgcgct gggacaaggc ggcctgccgc ttctgcggca
     4981 ccggctgctc ggtgctggtg ggcgtgcagg acgggcgcgt ggtcgccacc cagggtgatc
     5041 cggactcgcc ggtcaaccgc ggcctgaact gcatcaaggg ctacttcctg tccaagatca
     5101 tgtacggcga gga
//
LOCUS       DAOQNN010000229         5097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9878_length_5097_cov_10.000595, whole genome shotgun sequence.
ACCESSION   DAOQNN010000229 DAOQNN010000000
VERSION     DAOQNN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9878_length_5097_cov_10.000595"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..680
                     /gene="moaC"
                     /locus_tag="PKV97_11590"
     CDS             192..680
                     /gene="moaC"
                     /locus_tag="PKV97_11590"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNB06565.1"
                     /translation="MSTPESSPLTHFDADGQAHMVDVGAKAETRRVAVATGRIHMLPA
                     TFDMVKSGSAKKGDVLGIARIAAIQASKRTSELIPLCHPIPLTRVAVEFELDAAAHAI
                     RCTVTAETVGRTGVEMEALTAVNVGLLTIYDMCKAVDRGMRMEGIQLMEKLGGKSGHW
                     KA"
     gene            complement(715..966)
                     /locus_tag="PKV97_11595"
     CDS             complement(715..966)
                     /locus_tag="PKV97_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06566.1"
                     /translation="MSPVAARLPLVALALFGLAAVSLGAALLCFAGLGALLHPALSDP
                     MAGLAFLVSAIALAGTGAFPLVFARLARRDAGPQPPPGA"
     gene            complement(992..1726)
                     /locus_tag="PKV97_11600"
     CDS             complement(992..1726)
                     /locus_tag="PKV97_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNB06567.1"
                     /translation="MPIFPLSIHDLRFAPNGREVLAGVDLELSEEGITLVLGPNGAGK
                     SVLLRTICGLIEPSSGSMAWGGAGGTRPQQGVMMVFQRPMMLRMAVLDNVVLGLKPLG
                     VAGAERRRRGLEMLERVGLADRAGDSARQLSGGEQQRLALARAWLTRPRLLLLDEPTA
                     SLDPSASAAVERIIREIRTDGTRILMVTHNLGQATRLGDDIVFMAGGRVCEHTPVRAF
                     FARPRSEEARLFIQGELPWRMRFQGA"
     gene            complement(1735..2439)
                     /locus_tag="PKV97_11605"
     CDS             complement(1735..2439)
                     /locus_tag="PKV97_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB06568.1"
                     /translation="MSAFAATFSDALSLLAHFDHTVLEIVALSLQVSGAAVVLGTLIG
                     LPLGACIAVGRFPGKTTLSVLINGMMGLPSVVVGVVVYLILSRSGPFGALGMLYSPAA
                     MVVAQTLLVVPLMAAITRQVVEDVWARYAEELQVMRFSWWDSVTTLLHDCRHSLVVAV
                     LAGLGRAMSEVGAVMIVGGNIDGATRVMTTAIALETSKGDLALAIALGMVLIVIILAL
                     NALAYVLRQWAMRRFG"
     gene            complement(2553..2840)
                     /locus_tag="PKV97_11610"
     CDS             complement(2553..2840)
                     /locus_tag="PKV97_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNB06569.1"
                     /translation="MSEAYVVGHITVKNAEMWAEYRSKVPQTLVPWGAELVFRGRQVA
                     ALAGESPHPDIVVIRFPSVAAVNDWFSSPAYQALIPLREQAADVLLLSYES"
     gene            complement(2874..3182)
                     /locus_tag="PKV97_11615"
     CDS             complement(2874..3182)
                     /locus_tag="PKV97_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06570.1"
                     /translation="MAFINGPVPESFERRVTATAREFERDLRQAWPGVEGGAASLRFR
                     LQDGAQRLVIDIEPAGVRRLGLFELPQLIVRYGFGPGEEAARRALLARLDRAMQKGGG
                     "
     gene            complement(3187..3444)
                     /locus_tag="PKV97_11620"
     CDS             complement(3187..3444)
                     /locus_tag="PKV97_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNB06571.1"
                     /translation="MTIRIAFKLFASLSDYLPDGHRGNRLELEVDEGTTVAEMIRRYN
                     VPERSAHLVLVNGHFVPPSERARKRLAEGDELAIWPPIAGG"
     gene            complement(3467..4756)
                     /locus_tag="PKV97_11625"
     CDS             complement(3467..4756)
                     /locus_tag="PKV97_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB06572.1"
                     /translation="MKHLILGNGPAGVVAAETLRKVAPADDILMVGHEDAPPYSRMAI
                     PYLLEGNIDESGTWLRKDPGHFERLRIRQLRGRAVALDADKRKVLFDDGHFEHYDRLL
                     IATGSHPVRPPIPGVVLPSVHTCWTLADARAIAAHARPGARVLQLGAGFIGCIIMEAL
                     AKCGVQLTVVEMGDRMVPRMMTPKAGGMIKRWVEEKGIRVLTSAGVQRIDRGSEAGSD
                     APLVVSLSTGDRLDCDLVIVAAGVAPNVGFLDETPVHVAKGVLVDDHMETSVPGIFAA
                     GDVAEAPDLFTGAHLVSAIQPNAADQARIAATNMARDRVHESRLNGVLAINVLDTLGL
                     ISSSFGQWWGEKPEAGGSGVEMVDEARYRYLSLQFKDDVLVGATSIGLTQHVGALRGL
                     IHGQVKLGDWKERLLANPLAFVDAYVARSQQPMALAR"
     gene            complement(4989..5072)
                     /locus_tag="PKV97_11630"
     CDS             complement(4989..5072)
                     /locus_tag="PKV97_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNB06573.1"
                     /translation="MLPHYYEVRGWTPGGEPTAETRARLGL"
BASE COUNT          810 a         1834 c         1787 g          666 t
ORIGIN      
        1 agcacggggg ccggcgcagg cagggccagc gcgaggctga gcagcagggc gaggggtcgg
       61 cgcatcatcg gatgctatga tacccgccga tccgcgcccg ttcccccgcc gatgtatcga
      121 cgtgtagcgg cgcctcccgc cgcgtggcga ttgccgccgc ggcacacccg catcttctgc
      181 cctgccccac catgagcact ccggaatcca gtccgctgac ccacttcgac gccgacggcc
      241 aggcccacat ggtcgacgtc ggcgccaagg ccgaaacccg ccgcgtcgcg gtcgccaccg
      301 gccgcatcca catgcttccc gccaccttcg acatggtgaa gtcgggcagc gcgaagaagg
      361 gcgacgtgct cggcatcgcc cgcatcgccg cgatccaggc ctccaagcgc accagcgagc
      421 tgatcccgct ctgccacccc atcccgctca cccgcgtcgc ggtcgagttc gagctcgacg
      481 cggccgcgca cgccatccgc tgcacggtca ccgccgagac cgtcggccgc accggcgtcg
      541 agatggaggc gctcaccgcg gtcaacgtcg gcctgctcac catctacgac atgtgcaagg
      601 cggtggaccg cggcatgcgc atggagggca tccagctgat ggagaagctg ggcggcaagt
      661 cggggcactg gaaggcgtga ggggggcgcg gcgtcgggcg taaggagcga ggcctcaggc
      721 gccgggcggc ggctgggggc ctgcatcgcg ccgcgcgaga cgcgcgaaga ccaaggggaa
      781 ggcgccggtg ccggcgagcg cgatcgccga gacgaggaag gccagcccgg ccatcgggtc
      841 ggacaaggcg gggtgcagga gcgcgccgag accggcgaag cacagcagcg cggccccgag
      901 cgacacggct gcgagcccga acagggcaag cgcgacgagc ggcagacggg cggccaccgg
      961 actcatcgcg ccgcctcccg cagcgtcgat ctcatgctcc ctgaaaacgc atccgccatg
     1021 gcaattctcc ctggatgaag aggcgcgcct cctccgagcg cggacgggcg aagaaggcgc
     1081 gcaccggggt gtgctcgcac acccggccgc ccgccatgaa gacgatgtcg tcgcccaggc
     1141 gagtcgcctg accgaggttg tgagtgacca tgaggatgcg cgtgccgtcg gtgcggatct
     1201 cgcggatgat gcgctcgacc gccgcgctcg ccgaggggtc gaggctggcg gtgggctcgt
     1261 cgagcagcag caggcgcggc ctggtcagcc aagcgcgcgc gagcgcgaga cgctgctgct
     1321 cgccgccgga gagctggcgc gcactgtcgc cggcgcggtc ggcgaggccg acccgctcca
     1381 gcatctccag gccgcgccgg cgccgctccg cccccgccac cccgagcggc ttgagcccga
     1441 ggacgacgtt gtcgagcacg gccatgcgca gcatcatcgg gcgctggaag accatcatca
     1501 cgccctgctg cggccgcgta ccgccggccc cgccccaggc catgctgccc gagctcggct
     1561 cgatcagccc gcagatcgtg cgcagcagca cgctcttgcc tgcgccgttg gggccgagca
     1621 ccagggtgat gccctcctcg ctcagttcca gatccacgcc cgcgagcacc tcccgcccgt
     1681 tgggcgcgaa gcgcaggtcg tggatggaga gggggaagat gggcatcgcg gacctcaccc
     1741 gaagcgccgc atcgcccact gccgcagcac gtaggcgagc gcgttgagcg cgaggatgat
     1801 cacgatcagc accatgccga gcgcgatcgc cagcgccagg tcgcccttgc tggtctcgag
     1861 cgcgatcgcc gtggtcatga cccgggtggc gccgtcgatg ttgccgccga cgatcatcac
     1921 cgcgcccacc tcgctcatcg cccgtccgag gcccgcgagc accgccacca ccagcgagtg
     1981 gcggcagtcg tgcagcagcg tggtgacgct gtcccaccac gaaaagcgca tcacctgcag
     2041 ctcctcggcg tagcgcgccc acacgtcctc gaccacctgg cgggtgatcg ccgccatcaa
     2101 cggcaccacc agcagggtct gcgccaccac catcgccgcc ggcgaataca gcatgcccag
     2161 ggcgccgaag ggccccgagc gcgacaggat caggtacacc accacgccca ccaccaccga
     2221 gggcaggccc atcatgccgt tgatcagcac cgacagcgtg gtcttgcccg gaaagcgccc
     2281 gaccgcgatg caggccccga gcggcaggcc gatcagcgtg ccgagcacca ccgccgcgcc
     2341 gctgacctgc agcgacagcg cgacgatctc gagcaccgtg tggtcgaaat gggcaagcag
     2401 cgacagcgca tcggagaagg tggcggcgaa ggcggacatc ggcggggagg ataaccgaaa
     2461 gccgacgccg gccggaacgc cgggagcggc ttcagccggg cgccgccgcg gcgcgcgctg
     2521 gtgtcggagg ccggtgcgaa aggcgcccgc ggctagcttt cgtaggacag caggagcacg
     2581 tccgccgcct gctcccggag cgggatcagg gcctgatagg ctggcgacga gaaccagtcg
     2641 ttcaccgccg cgacgctggg aaagcggatc acgacgatgt ccgggtgggg gctttcgccc
     2701 gcgagcgcgg ccacctgccg gccgcgaaac accagctccg cgccccaggg gaccaaggtc
     2761 tgcggaacct tgctgcggta ttcggcccac atctccgcgt tcttcaccgt gatatggccg
     2821 acgacgtatg cttcactcat ggacgggctt ccagaaatcg attgtgctgc agatcaaccg
     2881 ccgcccttct gcatcgcgcg atcgagccgc gccagcaagg cgcggcgcgc ggcttcctcg
     2941 cccggaccga agccgtagcg cacgatcagt tgcggcagct cgaacagacc caggcggcgc
     3001 acgccggcag gctcgatgtc gatgaccagg cgctgcgcgc cgtcctgcag gcggaagcgc
     3061 aggcttgccg cgccgccctc cacccccggc caggcctggc gcaggtcgcg ctcgaactcg
     3121 cgcgcggtgg cggtgacccg gcgctcgaag gactcgggca ccgggccatt gatgaacgcc
     3181 acctgctcag ccacccgcga tcggcggcca gatggcgagc tcgtcgccct cggcgaggcg
     3241 cttgcgggcg cgctccgagg gcggcacgaa gtgtccgttc accagcacca gatgcgcgct
     3301 gcgctcgggc acgttgtagc gccggatcat ctccgccacc gtggtgccct cgtccacctc
     3361 gagttcgagg cggttgccac ggtggccgtc gggcagatag tccgacagcg aggcgaagag
     3421 cttgaaggcg atgcggatcg tcacgcgagt ctccaagatc gccccctcag cgcgccagcg
     3481 ccatcggctg ctggctgcgc gcgacgtagg catcgacgaa ggcgagcggg ttggcgagca
     3541 ggcgctcctt ccagtcgccc agcttcacct ggccgtggat cagcccgcgc agcgcgccca
     3601 catgctgggt gagcccgatc gaggtggcgc cgaccagcac gtcgtccttg aactgcaggc
     3661 tcaggtagcg gtagcgcgcc tcgtccacca tctcgacccc gctgccgccg gcctcgggct
     3721 tttcgcccca ccactggccg aaggacgagg agatcaagcc gagcgtgtcg agcacgttga
     3781 tggccagcac gccgttgagg cgcgactcgt ggacgcggtc gcgcgccatg ttggtggcgg
     3841 cgatgcgcgc ctggtcggcg gcgttgggct ggatcgccga caccaggtgc gcgccggtga
     3901 acaggtcggg cgcctcggcc acgtcgccgg ccgcgaagat gccgggcacg ctggtctcca
     3961 tgtggtcgtc gaccagcaca cccttggcca catgcaccgg cgtctcgtcg agaaagccga
     4021 cgttgggcgc cacgcccgcg gcgacgatca ccaggtcgca gtcgaggcgg tcgccggtgg
     4081 acaggctcac caccagcggc gcatcggaac cggcctcgct gccgcggtcg atgcgctgca
     4141 cgcccgccga ggtgagcacg cggatgccct tctcctcgac ccagcgcttg atcatgccgc
     4201 cagccttcgg cgtcatcatg cgtggcacca tgcggtcgcc catctccacc acggtgagct
     4261 gcacgccgca cttggcgagc gcttccatga tgatgcagcc gatgaagccg gcgcccagtt
     4321 gcagcacgcg cgcgccgggt ctggcatggg cggcgatcgc gcgcgcgtcg gcgagcgtcc
     4381 agcaggtgtg caccgagggc aggaccacgc cggggatggg cgggcgcacc ggatgcgagc
     4441 cggtggcgat cagcaggcgg tcgtagtgct cgaagtggcc gtcgtcgaac agcaccttgc
     4501 gtttgtcggc gtcgagcgcg accgcgcggc cgcgcagctg gcggatgcgc aggcgctcga
     4561 agtggccggg gtccttgcgc agccaggtgc ccgactcgtc gatgttgcct tcgagcaggt
     4621 agggaatcgc catgcgcgaa tacggcggcg cgtcctcgtg gccgaccatc aggatgtcgt
     4681 cggccggcgc caccttgcgc agggtttcgg cagcaacgac gcccgcgggg ccgttgccga
     4741 gaatcaggtg tttcattgca gcgtcctcta gcgaaccgcg gcgtggaagg caggccgcgg
     4801 caaggcgatc gcaggtaatc cgggtgatcc gggccccgta ggagcggcgc aagccgcgag
     4861 cccttcggat cgggccgagc gtgcgcggcg ccaaccgcgc ccgttcaaac cctcgcggct
     4921 ggcgccgctc ctgcagggca atccagcgcc atgggcgccc gcaggggcgt ccatgggcgt
     4981 ttgctgaatc acaagcccag gcgtgcccgt gtctccgcgg tcggctcgcc acccggcgtc
     5041 cacccgcgca cctcgtagta gtgcggcagc atcttgtcga gctggttcac caggccc
//
LOCUS       DAOQNN010000230         5096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9881_length_5096_cov_12.044832, whole genome shotgun sequence.
ACCESSION   DAOQNN010000230 DAOQNN010000000
VERSION     DAOQNN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9881_length_5096_cov_12.044832"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(475..1875)
                     /gene="dnaB"
                     /locus_tag="PKV97_11635"
     CDS             complement(475..1875)
                     /gene="dnaB"
                     /locus_tag="PKV97_11635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNB06574.1"
                     /translation="MSSASSRFSDFSADPQLAQIKLPPHSLEAEQSLIGGILLDNQAW
                     ERVADLVNEADFYRDDHRRIYRHITKLIDQGRPADAVTVFESLEKNGEAEQAGGLAYI
                     AELANNTPSAANIRRYAEIVRERAILRKLVAVGDEIAASALSPSGKDAKALLDEAEAK
                     VFEIAEAGARHASGFVSIQPILKQVVDRVQELYDRDNPSEVTGVPTGLVDLDEKTSGL
                     QPSDMIIVAGRPAMGKTTFALNLAEHIAIEQRLPVAIFSMEMPGTQLATRFISSVGRI
                     DMQKIRSGRLTDDDWQRLTMAMGKLYDAPLYIDETPGLNPIDLRARARRLARQCGRLG
                     LIVIDYLQLMSGTREGDNRASELSEISRSVKSLAKELNVPIMALSQLNRSLEQRPNKR
                     PVMSDLRESGAIEQDADIIMFIYRDEVYNPDSPDKGTAELIIGKHRNGPTGTVRLTFI
                     GENTRFENYAAAPGMY"
     gene            complement(2017..2466)
                     /gene="rplI"
                     /locus_tag="PKV97_11640"
     CDS             complement(2017..2466)
                     /gene="rplI"
                     /locus_tag="PKV97_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNB06575.1"
                     /translation="MQIILLEKVVNLGGLGDVVKVKDGYARNFLIPQGKAKRATQANL
                     AEFEARRAELERVQAEKLAAAQELAGKLEGVSVEVARKAGMDGRLFGSVGNADISEAL
                     AAKGFDIERSAIRMPDGPLKQVGETQLEVSLHADVLANITVVVAAEQ"
     gene            complement(2482..2757)
                     /gene="rpsR"
                     /locus_tag="PKV97_11645"
     CDS             complement(2482..2757)
                     /gene="rpsR"
                     /locus_tag="PKV97_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNB06576.1"
                     /translation="MAFKPKSKFKKKDDRGGRGLFKRRKFCRFTAEKIEEVDYKDVDI
                     LKDFITENAKIMPARITGTKAGYQRQLSVAVKRARFLALMPYTDLHQ"
     gene            complement(2760..3074)
                     /gene="priB"
                     /locus_tag="PKV97_11650"
     CDS             complement(2760..3074)
                     /gene="priB"
                     /locus_tag="PKV97_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HNB06577.1"
                     /translation="MPEPGLNELRLDARVAEVLPLRRTPAGIPVASCVLAHESKQVEA
                     GLVREVSVEVQAVALGDLAAVLAVAVPGSALRATGFLAAKSLRSRTPVLHLNTIEFLE
                     GN"
     gene            complement(3076..3462)
                     /gene="rpsF"
                     /locus_tag="PKV97_11655"
     CDS             complement(3076..3462)
                     /gene="rpsF"
                     /locus_tag="PKV97_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNB06578.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKSIVTTRNGQIHRLEDWGR
                     RQMAYPIQKVHKAHYVLMNIECDGETLAELEHSFKFNDAVLRHLTIKMKKAVTTPSPM
                     MKEEKSRSMNIGADDAKPADSEAAAA"
     gene            3701..4033
                     /locus_tag="PKV97_11660"
     CDS             3701..4033
                     /locus_tag="PKV97_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06579.1"
                     /translation="MNTTLTRKIATALLCGSVLAAAAPAAYADRDDRGPRWGHHKHHY
                     KHDHRRHWDERTVYREKVIIRERPRYYREAEIHNHYYERPYRSYSYSRSPAVVIGVDI
                     PPLVIPLR"
     gene            complement(4113..>5096)
                     /locus_tag="PKV97_11665"
     CDS             complement(4113..>5096)
                     /locus_tag="PKV97_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HNB06580.1"
                     /translation="DARDAAADVGGRAGAGQPGGKGSGKAAGDAAGRSAAPAGLPAQV
                     LSGFTAWTFGALPELLEVRVAGREVIGFPALHDDGDSVSLRPHDTPEEASRVHRRGLA
                     RLFALNLKDQVRAVERLPGLRELALQYMGFGTEAELKARLIEATLGRCCLLEPLPTDA
                     DAFAKRCAEAKSRVSLVAQEFMRLTGQLLVEHAALQKRLSGLKTFPEVVADIQGQLGA
                     LLPKDFLVAFEWDKLAHFARYLKGAAVRLDKLRNNPARDAQAMAEWKQLAQAWERERL
                     ARRRAGVEDPALEEFRWLLEELRVGLYAQELRTPMPVSVKRLQKIWESRPR"
BASE COUNT          902 a         1730 c         1599 g          865 t
ORIGIN      
        1 cgccgaccac aacaaccaag cacccacact tatcggttgt ccaagttttt aaagaatcgc
       61 tgcgttgccg cagcgaagaa gcgagattat gtagaaccta catcaccctg tcaagcatct
      121 cgcgaatctt tttttgcgcc gccccgccaa aaacacggaa caacaagacc cgcaacaccc
      181 gaccgcctct ccccttcccg caccgctgca cctgcactca agcaccgctg cgctggaaga
      241 ggtgcgcatt gtagacaacc aaatcctgac gtcaagaaaa aaacaacaaa gattccgatg
      301 aaagcatgcg agcaacgccg acgggacccc gggaccgatc accgagcaca acacacctct
      361 ggcgccagcg tctctacggg cctcccgcag ggcccgacgc aggaacaatc gcttggtgac
      421 gaccgccgac cttcctgcgc aaaccgcaaa atcgcgacca tcctgcgata gggctcagta
      481 catgccgggg gcggcggcgt agttctcgaa gcgcgtattc tccccgatga aggtgaggcg
      541 cacggtgccg gtgggaccgt tacgatgctt gccgatgatc agttcggccg tccccttgtc
      601 gggcgaatcg gggttgtaga cctcgtcgcg atagatgaac atgatgatgt ccgcgtcctg
      661 ctcgatggca ccggactcgc gcagatcgga catcaccggg cgcttgttgg gacgctgctc
      721 gaggctgcgg ttgagctggg aaagcgccat gatcggcacg ttcaactcct tggccagcga
      781 ctttaccgaa cgcgagatct cggacagctc ggaggcacgg ttgtcgccct cgcgcgtgcc
      841 gctcatcagc tgcaggtagt cgatcacgat cagaccgagc ctgccgcact ggcgcgccag
      901 gcgacgcgca cgggcgcgca ggtcgatcgg gttgaggccg ggcgtctcgt cgatgtagag
      961 cggcgcatcg tagagcttgc ccatcgccat ggtcagacgc tgccagtcgt cgtcggtcag
     1021 gcgtccactg cggatcttct gcatgtcgat ccgacccacc gaggagatga agcgagtcgc
     1081 gagctgcgtg cccggcatct ccatcgagaa gatcgcaacc ggcaggcgct gctcgatcgc
     1141 gatgtgctcg gcaaggttga gcgcgaaggt ggtcttgccc atcgccggac ggccggcgac
     1201 gatgatcatg tcggacggct gcaggccgga ggtcttctcg tcgaggtcga ccaggccggt
     1261 cggcacgccg gtgacctcgg aggggttgtc gcggtcgtag agttcctgca cgcgatccac
     1321 gacctgcttg aggattggct ggatcgagac gaaaccgctg gcgtggcgcg cgccggcctc
     1381 ggcgatctcg aagaccttgg cctcggcctc gtcgagcagc gccttggcgt ccttgcccga
     1441 cggactgagc gcactggcag cgatctcgtc tcccaccgcc acgagcttgc gcaggatcgc
     1501 gcgctcgcgg acgatctccg cgtagcgccg gatgttcgcc gccgacggcg tgttgttggc
     1561 gagctcggcg atgtaggcga gaccgccggc ctgctcggcc tcgccgttct tctccagcga
     1621 ctcgaacacg gtgaccgcat ccgccggtcg cccctggtcg atcagcttgg tgatgtggcg
     1681 gtagatgcgg cggtggtcgt cgcgatagaa atcggcctcg ttcaccaggt cggcgacacg
     1741 ctcccaagcc tggttgtcga gcaggatgcc gccgatcagc gactgctcgg cctccagcga
     1801 gtgcggcggc agcttgatct gcgcgagctg aggatcggcg gagaaatcgg agaaacggga
     1861 agacgcggaa ctcatcggat gcaccatcgg ggccggaccg cacattgtag cgagccggac
     1921 cgcgagacgt tggcgaaggg aacgcgccgc aggggcgcag gaaacaaaaa gggctgccag
     1981 cggcagccct cttcgtgatg cacgtacaga tcgcgattac tgctcggcag ccaccacgac
     2041 ggtgatgttc gcgagcacgt cggcgtgcag cgacacttcg agctgagtct cgccgacctg
     2101 cttgagcggg ccgtccggca tgcggatggc ggaacgctcg atgtcgaagc ccttggcggc
     2161 gagcgcctcg gagatgtcgg cattgccgac cgagccgaac agacggccgt ccatgcccgc
     2221 cttgcgagca acttcgacgc tgacgccttc gagcttgccc gccagctcct gggccgcagc
     2281 cagcttttcg gcctggacgc gctcgagctc ggcacgacgc gcttcgaact cggccagatt
     2341 ggcctgggtc gcgcgcttcg ccttgccctg cgggatcagg aagttgcggg cgtagccgtc
     2401 cttgaccttt acgacgtcgc cgagaccacc gaggttcacc actttctcga gaagaatgat
     2461 ctgcatgggt tcgttctcct cttactggtg caggtcggtg taaggcatca gggccaggaa
     2521 gcgggcgcgc ttgacagcga ccgacagctg acgctggtaa cccgccttgg tgccggtgat
     2581 gcgcgccggc atgatcttgg cgttttcggt gatgaagtcc ttgaggatgt ccacatcctt
     2641 gtagtcgact tcctcgatct tctccgcggt gaagcggcag aacttgcgac gcttgaacag
     2701 accacgaccg ccgcgatcgt ccttcttctt gaacttggat ttgggcttga aggccatttt
     2761 cagtttcctt cgagaaattc gatagtgttc aggtgcagaa ccggagttcg gctgcgcagg
     2821 ctcttcgcgg cgaggaatcc ggtggcgcgc agcgcactcc cgggaaccgc cacagccagc
     2881 accgccgcca gatcgcccag cgccaccgcc tgcacttcca ccgaaacctc gcggaccagg
     2941 cccgcttcga cttgtttcga ttcgtgcgcc agcacacagc tggcgaccgg tattccggca
     3001 ggggttcgac gcagcggcag cacttcggcc acgcgcgcat cgaggcgcag ttcgttcaga
     3061 cccggctcag gcaactcagg cggcagcggc ttcgctgtcg gccggcttgg cgtcgtcggc
     3121 accgatgttc atcgagcgcg acttctcttc cttcatcatc ggcgaaggcg tggtgacggc
     3181 cttcttcatc ttgatggtga ggtgacgcag cacggcgtcg ttgaacttga acgagtgctc
     3241 gagttcggcc agcgtctcgc cgtcgcactc gatgttcatc agcacgtagt gggccttgtg
     3301 caccttttgg atcgggtagg ccatctggcg gcgcccccag tcttccaggc ggtggatctg
     3361 gccgttgcgg gtggtcacga tggacttgta gcgctcgacc atcgccggca cctgctcgga
     3421 ctggtccggg tgcacgatga agacgatttc ataatgtcgc atgcaaactc cttgcggttg
     3481 gttgttgcag ccccccggca tgcggtccgg tggagcaagg gaagctcgcc agtctaacaa
     3541 gaaaacccgg aaaatcaaag acactgaaga agcccggaag tcgggagcgc ggttgcaaaa
     3601 gcccgcttac actctgcgac tgcctcgtgg cgtccgcctc gcctagtatc cggatcaccg
     3661 gggccctgcc ctgcaacgaa gaaatcgaac gaggcagacc atgaacacga ccctgacccg
     3721 caagatcgcc accgccctgc tctgcggcag cgtcctcgcc gctgccgcgc ctgccgccta
     3781 cgccgaccgg gacgaccgcg gcccgcgctg gggccaccac aagcaccact acaagcacga
     3841 tcatcgccgc cactgggacg agcgcaccgt gtatcgcgag aaggtcatca tccgtgaacg
     3901 cccgcgctac taccgcgagg cggagatcca caaccactac tacgagcgcc cctaccgctc
     3961 ttacagctac agccgctcgc ccgccgtggt catcggcgtg gatatcccgc cgctggtgat
     4021 cccgctgcgc tgaacacccc ccgctccatc cccgttcgca gggcgcggcc tcactcccct
     4081 gacggcacgc ccggccgagc cggtcgcgcg actcagcgcg gccggctctc ccagatcttc
     4141 tgcagacgct tcaccgacac cggcatcggc gtcctcagct cctgcgcgta caagcccacg
     4201 cgcaactcct ccagcagcca gcggaactct tccagcgccg gatcttccac gcccgcgcgc
     4261 cggcgggcga ggcgctcgcg ctcccaggcc tgggcgagct gcttccactc cgccatggcc
     4321 tgggcgtcgc gcgccggatt gttgcggagt ttgtcgagcc gcaccgcggc acccttgaga
     4381 tagcgtgcga agtgcgccag cttgtcccac tcgaaggcga cgaggaagtc cttcggcagc
     4441 agcgcaccga gctggccctg gatatcagcc accacctcgg gaaaggtctt cagcccggac
     4501 aggcgcttct gcagcgccgc atgctcgacc agcagctggc cggtcaggcg catgaactcc
     4561 tgcgcaacca ggctcacccg cgacttggcc tcggcgcagc gcttcgcgaa cgcgtcggca
     4621 tccgtaggca aaggctcgag caggcagcag cggcccagcg tcgcctcgat caggcgcgcc
     4681 ttcagctcgg cctcggtgcc gaagcccatg tactgcagcg cgagctcgcg caagccgggc
     4741 agacgctcga ccgcgcgcac ctgatccttg aggttgagcg cgaacaggcg ggcgaggccg
     4801 cggcgatgca cgcgcgacgc ctcctcgggc gtgtcgtggg ggcgtaacga gacgctgtcg
     4861 ccgtcgtcgt gcagcgcagg aaaaccgatc acctcacggc cggcgacgcg cacctcgagc
     4921 aactcgggca gggcgccgaa agtccacgca gtgaagccgg acagcacctg cgccgggagt
     4981 ccggcaggcg cagcgctacg gcctgccgcg tcgcccgccg ccttgccgct ccctttgccc
     5041 cccggctgcc ccgccccggc acggccaccc acatccgccg cggcatcacg cgcatc
//
LOCUS       DAOQNN010000231         5090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9891_length_5090_cov_11.057994, whole genome shotgun sequence.
ACCESSION   DAOQNN010000231 DAOQNN010000000
VERSION     DAOQNN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9891_length_5090_cov_11.057994"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PKV97_11670"
     CDS             complement(<1..389)
                     /locus_tag="PKV97_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNB06581.1"
                     /translation="MFEKILIANRGEIALRILRACRELGIKTVAVHSEADTHAKYVRL
                     ADESVCIGPPPSAQSYLNVPAIISAAEVTDAEAIHPGYGFLSENADFGERVEQSGFVF
                     IGPRPETIRLMGDKVSAKDAMKAAGVP"
     gene            complement(397..849)
                     /gene="accB"
                     /locus_tag="PKV97_11675"
     CDS             complement(397..849)
                     /gene="accB"
                     /locus_tag="PKV97_11675"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNB06582.1"
                     /translation="MDLRKLKKLIDLVQESGISELEVTEGEEKVRIAKHTSAPPATYY
                     AQVPPAPAAAPAAVVASPALTDAEDELPDGHVVKSPMVGTFYRAGAPGAKPLVELGQK
                     IATGDRLCIIEAMKLMNEIEADASGVVKAILVENGEPVEYGQPLFVIG"
     gene            complement(1295..1918)
                     /locus_tag="PKV97_11680"
     CDS             complement(1295..1918)
                     /locus_tag="PKV97_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06583.1"
                     /translation="MPSHAHVSRSGGLRREIAALAARMMAEDGISDFGFAKRKAAKQL
                     GATDADALPNNNEVEAELRAWLAIYQDEEQPQRLLEMRSAAIEVMRLLADFRPYLTGG
                     ALDGTAGRYSELEIELFPESTKDVEIFFLNQDFAYEHREPRRPAPHTPEAILSFDWDD
                     VPVKVSIYPSAAERSPRRGQDRARLAQVEALVAAAVESTRDTGSHAP"
     gene            2002..3348
                     /gene="mpl"
                     /locus_tag="PKV97_11685"
     CDS             2002..3348
                     /gene="mpl"
                     /locus_tag="PKV97_11685"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HNB06584.1"
                     /translation="MHIHILGICGTFMGGVALLARAAGHTVTGCDANVYPPMSTQLEE
                     QGIGLIEGYDTSQLDLEPDMYVVGNAVSRGNPLLEAILDRGLPYVSGPQWLAEHVLAG
                     RWVLAVAGTHGKTTTTSLLAWMLEDAGLSPSFLVGGVPQNFGVSARLTESPFFVIEAD
                     EYDTAFCDKRSKFVHYRPRTAILNNLEFDHADIFADLGAIETQFHHLVRTIPASGRIV
                     ANAHEDSLKRVIERGCWSELEWFGGDGRWTVGTGADEAEAVFRLDGVEQGRVRMALAG
                     SHNRDNALAAIAAARHVGVTPAQAIASLGRFAGIKRRLELRGTVRGVSVYDDFAHHPT
                     AIALTVGGLRRREPQGRILAVLEPRSNTMKLGVMKAQLPASLAEADLVFCHAHGLDWD
                     AAAALAPLGARARTYDRLDALVAAVVAAAQPGDHVLVMSNGGFGGVHQKLLDALGA"
     gene            complement(3410..4069)
                     /locus_tag="PKV97_11690"
     CDS             complement(3410..4069)
                     /locus_tag="PKV97_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNB06585.1"
                     /translation="MTRPQHADRPAKSSRRTLLLGLSAAIALPLLQGCFPVVATGVGA
                     GAAMVSDRRTSGTYVEDEGIEWKVAARIRERLGDSVHVNVTSYNRNVLLTGEAPNETV
                     RGQLDGIVAGVENVRGVINEVAIAPNSSLTARGNDTLITSNVKARMIDSGAVSAHNVK
                     VVTEANVVFLLGIVPRSEADAAAEVARTSQGVRKVVRVFEYISDEEARRLDNPTGARR
                     N"
     gene            complement(4069..4662)
                     /locus_tag="PKV97_11695"
     CDS             complement(4069..4662)
                     /locus_tag="PKV97_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNB06586.1"
                     /translation="MNLIDRISHQFEDNMRASLEALELLAAPIAGAVELITASLLDSG
                     KVLVCGSSGSAGDARRFAAQLVNGFELERPALAAMALSADSSSLAPIAGDPDTQALFA
                     RQIAAFGQPGDILLTLSANPDSARVLDAIRAAHERDMRVIALTGAGGGRTAETLGPAD
                     ILLCAPAERIARIREIHLLILHCLCDGIDCLLLGVED"
     gene            complement(4707..>5090)
                     /locus_tag="PKV97_11700"
     CDS             complement(4707..>5090)
                     /locus_tag="PKV97_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNB06587.1"
                     /translation="DSIGARPTPAQARGAAAEALAAEHLAAHGLRVIARNVRCRGGEV
                     DLVCLDRSHVVFVEVRLRRNNRFGGAAESITAAKQRRVLIAAQWWLGGAGRRFRDAAC
                     RFDAVLLDALDPARIIWLPGAFDAG"
BASE COUNT          727 a         1658 c         1892 g          813 t
ORIGIN      
        1 cagggcacgc cagcggcctt catcgcatcc ttggcagaga ccttgtcgcc catcaggcgg
       61 atggtctcgg ggcgtgggcc gatgaagacg aagcccgact gctcgacgcg ctcgccgaag
      121 tcggcgttct cggagaggaa gccgtagccg gggtggatgg cctcggcgtc ggtgacctcg
      181 gcggcagaga tgatggccgg cacgttgagg tagctctgcg cagagggcgg cgggccgatg
      241 caaacggact cgtcggccag tcgcacgtac ttggcgtggg tgtcggcctc ggagtggacc
      301 gcgaccgtct tgatgccgag ctcgcggcag gcgcgcagga tgcgcagggc gatttccccg
      361 cggttggcga tcaggatctt ctcgaacatg gcggactcag ccgatcacga acaggggctg
      421 gccgtattcg acgggctcac cgttctcgac caggatggcc ttgacgacgc cgctggcgtc
      481 ggcctcgatc tcgttcatca gcttcatcgc ctcgatgatg cacaggcggt cgccggtggc
      541 gatcttctgg ccgagttcga ccagcggctt cgcgcccggg gccccggcgc gatagaaggt
      601 cccgaccatg ggcgacttga ccacgtgacc gtcgggcaac tcgtcttcgg catcggtcag
      661 ggcgggggag gcgacgacgg ccgccggtgc ggctgcgggt gcggggggga cctgggcata
      721 gtaggttgcc ggaggtgcgc tggtgtgctt ggcgatgcgt accttttcct cgccttcggt
      781 cacctcgagc tccgagatgc cggactcctg gacgagatcg atgagtttct tgagcttgcg
      841 cagatccatg gtgttctccg gacgggaccg cgatcgcctc gacgggcgcc acgatcggga
      901 tgattcagtg ttcgggtttc gcgcacacgc cgaggccgcc gcggtcgggc ggcggactgg
      961 tgcggaaacg tttcgtgctg agggggccgc gcgcgaactc gctcggccgg gatggctcgc
     1021 caccacgggc ggaatgcgtg caagcgacct cgtgaagcac cgccccgtca tgccgggggg
     1081 ctggcccgag cgcccggtga agatgggtcg tcaagcattt tgagcgcttc atggatgcaa
     1141 gtttgccgta aatcgcggca aattgtccac tttgggttgt gatctggact tgcgtggagg
     1201 gtgcctggga cccggcgccg tgcgcggatt ctgcgctcgt gcgccgtggg ctgcaagccg
     1261 cggagggtgc ccggcatggc ggggcaggca aacctcaggg agcgtgcgac cccgtgtcgc
     1321 gtgtgctctc caccgccgcg gcgaccaggg cctcgacctg ggcgaggcgg gcgcgatcct
     1381 ggccgcggcg cgggctgcgc tcggcggccg agggatagat cgacaccttc accggcacgt
     1441 cgtcccagtc gaagctgagg atggcctcgg gcgtatgcgg tgcggggcgg cgcggctcgc
     1501 ggtgctcgta ggcgaagtcc tggttgagga agaagatctc gacgtccttg gtgctctcgg
     1561 ggaagagttc gatctcgagt tccgagtagc gtccggcggt gccgtcgagg gctccgccgg
     1621 tgaggtaggg gcgaaagtcg gcgagcaggc gcatgacctc gatcgccgcg ctgcgcatct
     1681 cgagcaggcg ctgcggctgc tcctcgtcct ggtagatcgc aagccaggcg cgcagctcgg
     1741 cctcgacctc gttgttgttg ggcagggcgt cggcgtcggt ggcgccgagc tgtttcgcag
     1801 ccttgcgctt ggcgaagccg aagtcgctga tgccgtcctc ggccatcatg cgtgcggcga
     1861 gcgcggcgat ctcgcgccgc aggccgccgc tgcgtgatac gtgggcgtgt gagggcatgg
     1921 tgggcctcgg gtagaatgcg cgcccattgt aatacacccc cggttgcggc cggatgaagg
     1981 cttagcggag cgcgaacccc catgcacatc catatcctcg gcatctgcgg caccttcatg
     2041 ggcggcgtcg cgctgctcgc gcgcgccgcc ggccacaccg tcaccggctg cgacgccaac
     2101 gtctacccgc cgatgagcac gcagctcgag gagcagggca tcgggctgat cgagggctac
     2161 gacaccagcc agctcgacct cgagcccgac atgtacgtcg tcggcaacgc ggtgtcgcgc
     2221 ggcaatccgc tcctcgaggc gatcctcgac cgcggcctgc cctacgtgtc cgggccgcag
     2281 tggctggccg agcacgtgct cgccgggcgc tgggtgctgg cggtcgccgg cacccacggc
     2341 aagaccacca cgacctcgct gctcgcctgg atgctggagg atgcgggttt gtcgccatcc
     2401 ttcctggtgg gcggcgtgcc gcagaacttc ggcgtgtccg cgcgcctgac cgagtcgccc
     2461 ttcttcgtga tcgaggccga cgagtacgac accgcgttct gcgacaagcg ctcgaagttc
     2521 gtgcattacc ggccgcgcac cgcgatcctc aacaatctcg agttcgacca cgccgacatc
     2581 ttcgccgacc tcggcgcgat cgagacccag ttccaccatc tggtgcgcac catcccggcg
     2641 agcggccgca tcgtcgccaa cgcccacgag gacagcctga agcgcgtcat cgagcgtggc
     2701 tgctggtcgg agctggaatg gttcggcggc gacggccgat ggacggtggg caccggggcg
     2761 gacgaggccg aggcggtgtt ccgcctcgat ggcgtcgagc agggccgggt gcgcatggcg
     2821 ctcgccggca gccacaaccg cgacaacgcg ctcgccgcga tcgccgccgc gcgccacgtc
     2881 ggcgtcacgc ccgcgcaggc gatcgccagc ctcgggcgtt tcgccggcat caagcgccgg
     2941 ctggaactgc gcgggacggt aagaggtgtg agcgtctatg acgacttcgc ccaccacccg
     3001 accgcgatcg cgctcaccgt cggcggcctg cgccggcgcg aaccgcaggg tcgcatcctg
     3061 gcggtgctcg agccgcgctc gaacaccatg aagctgggcg tcatgaaggc gcagttgccg
     3121 gccagcctcg ccgaagccga tctggtgttc tgccatgcgc acgggctgga ctgggatgcc
     3181 gccgcggcgc tcgccccgct gggcgcacgc gcgcgtacct acgatcgcct ggatgcgctg
     3241 gtcgccgccg tggttgcggc ggcacagccc ggcgaccacg tgctggtgat gagcaacggc
     3301 ggcttcggcg gcgtgcacca gaagctgctc gacgcgctcg gagcgtaagt cgcatccctg
     3361 gaagcgcgtt cggaaggacg ccgccgccag ggatgccggt cgggggcgct cagttgcggc
     3421 gcgcgccggt cgggttgtcg aggcggcggg cttcctcgtc gctgatgtat tcgaacaccc
     3481 gcacgacctt gcgcacgcct tggctggtgc gcgccacctc ggcggcggcg tcggcctcgc
     3541 tgcgggggac gatgccgagc aggaagacga cgttggcctc ggtcaccacc ttgacgttgt
     3601 gggccgagac ggccccggag tcgatcatgc gcgccttcac attggaggtg atcaaggtgt
     3661 cgttgccgcg cgcggtgagg ctggagttgg gcgcgatggc gacttcgttg atcacgccgc
     3721 gcacattctc gacgccggcc acgatgccat cgagctggcc gcgcacggtc tcgttgggcg
     3781 cttcgccggt gagcagcacg ttgcggttgt aggaggtgac gttgacatgc acgctgtcgc
     3841 cgaggcgctc gcggatgcgc gccgccacct tccattcgat cccttcgtcc tcgacatagg
     3901 tgccgctggt gcgacggtcg gagaccatcg ccgcacccgc gccgacaccg gtggcgacga
     3961 ccgggaagca gccctgcagc aagggcagtg cgatggctgc ggaaaggccc aggaggaggg
     4021 tgcgccggct ggacttcgcc ggtctgtcgg cgtgttgggg tcgggtcatc agtcttccac
     4081 tccgagtaag aggcagtcga taccgtcgca caggcagtgc aggatcagca agtggatttc
     4141 ccggatgcgg gcaatccgct cggcgggtgc gcagagcagg atgtcggccg ggccgagcgt
     4201 ctcggcggtg cgcccgccgc cggcgccggt gagggcgatc actcgcatgt cgcgctcatg
     4261 ggcggcgcgg atggcgtcga gcacgcgcgc cgaatcgggg ttggcggaca gggtcagcag
     4321 gatgtcgccg ggctgcccaa aggcggcgat ctgacgggcg aacaaggctt gggtatcggg
     4381 gtcgccggcg atcggggcga ggctggagct gtcggcgctc agcgccatcg cggcgagcgc
     4441 cgggcgttcg agctcgaagc cattgaccag ctgagcggcg aaacggcgcg cgtcgccggc
     4501 cgaaccgctg ctgccgcaca ctagcacctt gccgctgtcg agcaggctgg cggtgatcag
     4561 ctcgaccgcg ccggcgatcg gcgcggcaag cagctcgagc gcctcgagcg aggcgcgcat
     4621 gttgtcttcg aactggtgcg agatgcggtc gatcaggttc atgcggcaag cggcgtctgc
     4681 ggggcgccag agtctagcac gcgctatcag ccggcatcga aggcccccgg gagccagatg
     4741 atgcgcgccg ggtcgagcgc gtcgagcagc accgcgtcga agcggcaggc tgcgtcgcga
     4801 aagcgccgcc cggcgccgcc cagccaccac tgcgcggcga tcagcacgcg gcgctgcttg
     4861 gccgcggtga tgctctcggc ggcgccgccg aagcggttgt tgcggcgcag gcgcacctcg
     4921 acgaagacga cgtggctgcg gtcgaggcag accaggtcga cctcgccgcc gcggcagcgc
     4981 acgttgcgcg cgatcacgcg caggccgtgt gcggcaaggt gttcggcggc gagcgcctcg
     5041 gcagcggcgc cgcgcgcttg cgccggcgtc gggcgtgcgc cgatactgtc
//
LOCUS       DAOQNN010000232         5078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9926_length_5078_cov_10.787776, whole genome shotgun sequence.
ACCESSION   DAOQNN010000232 DAOQNN010000000
VERSION     DAOQNN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9926_length_5078_cov_10.787776"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PKV97_11705"
     CDS             <1..791
                     /locus_tag="PKV97_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HNB06588.1"
                     /translation="FEAPLRFHHGIALPAPELHAEALLFAARRLFASLAAWLDARHAA
                     VDRFRLRFGHGSRPPTELEFALGRPSRDEGHFALIVRERLAMLELSAEVDSITLSAES
                     PVAADPRARDLFGDPAQAGEDAALLLARLQARLGEECVQRLDLRADHRPEAAWRNTVP
                     SAQAATARAGRDAAAGPASTRTASPPRTTAGLRPLWLLPAPRPISAPRYTLLGAAERI
                     ETGWWDGMPVRRDYYLARDADNVLCWIFESLDAPGQWFVHGYFG"
     gene            1045..1629
                     /locus_tag="PKV97_11710"
     CDS             1045..1629
                     /locus_tag="PKV97_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06589.1"
                     /translation="MPEPSSSVTVSAETRARPLPGDLDRRRALFALLVCVVALPASWL
                     LFSQLERLWPEIVRLEGLGFLAAATLLGGVMAIGPLAAALGFLTAVWFGVRSVYSARS
                     LATPLLDRVIVGAGLLVWFMPALAAIAQAVRALTTGRIHFVRPPRDYLLATDPIAFWQ
                     GVGFWLIIAGLAGFLAWRYWQPKLFPAHDAAPEA"
     gene            complement(1729..3576)
                     /locus_tag="PKV97_11715"
     CDS             complement(1729..3576)
                     /locus_tag="PKV97_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HNB06590.1"
                     /translation="MSARSTRLTAAASALHDRPPPAVEYRIRPANPGAHLFEVSCTVA
                     EPDPAGQVFSLPAWIPGSYMIREFARNIVRLRAEADGEPCALEKLDKHTWRAAAVPGA
                     RVLSVHYEVYAWDLSVRTAHLDTTHGFFNGTSVFLAVAGRTEAPCVVTIERPEGDCGR
                     DWKLVTALPPEHGRPGQACRFGRFRAADYDELIDHPVEMGRFTLARFEAAGVPHDIAL
                     TGRHDCDLERLCADLRRICEWQIALFGTPAPVDYYAFLTMVVGEGYGGLEHRASTALI
                     CSRAELPWKGMEGLPDGYKSFLGLCSHEYFHTWNVKRIKPAAFTPYDLARENHTRLLW
                     AFEGFTSYYDDLALVRSGVIGIDDYLGLLGKTIANVLRGSGRLKQSVAESSFDAWTKY
                     YRQDENAPNAIVSYYAKGALIALALDLQLRAGSEGAASLDDVMRLLWRRHGLTGVGVP
                     EDGIFAAVRDAGGERLGARLAKWLQKAVDGCEDLPLARLLRPFGVSLRAEAAGTAPVL
                     GMKLGGGSGEAKVANVYDDGPAQAAGVSAGDVLIALDGLRISSAKGLEDLLARRGAGD
                     EVELHLFRRDELMSFRAVLAAPPAERQELKLAPRADSAAAKLRRGWLGG"
     gene            complement(3750..>5078)
                     /locus_tag="PKV97_11720"
     CDS             complement(3750..>5078)
                     /locus_tag="PKV97_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06591.1"
                     /translation="QLAFINPEAPAFAIPLVASQAGDLISKTANEVATDNGTTVNRSY
                     LSRQVLSLSNEVTDFTGLKQASDGLISADGTEQGRLMATRRQVDAICAEFFSKCPTAK
                     KLSDDLAHYIELVATRNAKIAEYNDALTELCYLRSQTDAIRSAKNKTESWRVSQAGLS
                     LPQMAAFSSALWRHAREQCVETLYLASRAFTMHTLSNKDVFVDVLRQLMAGSQRPDNI
                     NSTAFRAALIDFIVGRLKEENKRLSTIQAFVPMGNSCRIEFTKARNPLLFATLMNGNE
                     GLVSILPARKDTNTDHTHFPGMANVRLSAIRACALGMRTKDNIHVIQFTHPGVETIVT
                     ASDVPVTVNHQKVHHEYRYDARALSPDGTTISAGAAAAARPETGTVTSAPAATPAPVL
                     TGGFLDAQRQMIGPFCQWSIRIPREYNSELDLSGLRSIIIEFEGTYQSFA"
BASE COUNT          735 a         1797 c         1691 g          855 t
ORIGIN      
        1 ggttcgaagc gccgctgcgc ttccaccacg gcatcgccct gcccgcgccg gaacttcacg
       61 ccgaggccct gctcttcgcc gcacgccgcc tcttcgccag ccttgccgcc tggctcgatg
      121 cccgccacgc ggcagtcgac cgcttccgcc tgcgtttcgg ccacggctcg cgaccgccca
      181 ccgaactcga gttcgcgctc ggccgcccaa gccgcgacga ggggcacttc gccctgatcg
      241 tgcgcgaacg cctggccatg ctcgagctgt ccgccgaggt cgactcgatc accctgagtg
      301 cggaaagccc cgtggctgcc gacccgcgcg cccgggacct gttcggcgat cctgcccagg
      361 ccggagagga cgccgccctg ctgcttgccc gcctgcaggc gcgacttggc gaggaatgcg
      421 tgcagcgcct ggatcttcgc gccgatcacc gtcccgaagc cgcctggcga aacaccgtcc
      481 cctccgcaca ggcggccact gcacgcgccg gccgcgacgc cgccgcgggg ccggcctcga
      541 cacggacggc atcgccgcca cgcacgacag caggcctgcg cccgctctgg ctgctgcccg
      601 cgccgcgtcc gatctccgct ccccgctata ccctgctcgg cgccgccgaa cgcatcgaga
      661 ccggctggtg ggacggcatg ccggtccgcc gcgactacta cctcgcgcgc gatgccgaca
      721 acgtcctgtg ctggatcttc gagagcctgg atgcgccggg gcagtggttc gtgcatgggt
      781 atttcggctg aagacgccgg tcccgatcac ggtgccggag cccgcgacga gggcggcgcg
      841 agccgggacg gaggatcgcc actcgcgtcg ctcccagcgg gatccgaagc tgcatcgtgc
      901 tgcggtaacg tgccgatttt cgtgggagcg acgcaagtcg cgatcacgac gacgcgcgag
      961 cgcgtgacgg gaactgcagg ggcgcggggc tgtgcgcgta gtacacttcc cgggctccga
     1021 accttttcca ccgcctccat tcccatgccc gagccttcct cttccgtcac cgtttccgct
     1081 gaaacccgcg cccgccccct gccgggcgac ctcgaccggc gccgcgcgtt gttcgcgctg
     1141 ctggtatgcg tggtcgcgct gcctgcatcg tggctgttgt tcagccagct cgagcgcctg
     1201 tggccggaga tcgtgcgcct ggaggggctc ggcttcctcg cggcggccac cctgctcggc
     1261 ggcgtgatgg cgatcggccc gctcgctgcg gcgctcggct tcctgaccgc ggtgtggttc
     1321 ggggtgcgca gcgtctacag cgcgcgcagc ctcgccacgc cgctgctcga ccgcgtcatc
     1381 gtcggcgccg ggctgctggt ttggttcatg cccgcgctgg cggcgatcgc acaggcggtg
     1441 cgcgcgctga ccacagggcg gatccacttc gtgcgcccgc cgcgcgacta cctcctcgcc
     1501 accgatccga tcgccttctg gcagggcgtc ggcttctggc tgatcatcgc ggggctcgcc
     1561 ggcttcctcg cctggcgcta ctggcagccg aagctgttcc cggcgcacga cgccgccccc
     1621 gaggcctgaa cgcgacctgc cgccgcgggc gcgcctggcc gtgcgtgccc gctccgtcac
     1681 accttgccgt cgtccgcggc tgcgcctcgc gcacggcaag gcggcgcatc acccccccaa
     1741 ccaaccccgc cgcagcttcg ctgccgcgct atcggcgcgc ggcgccagct tgagctcctg
     1801 gcgctcggca ggcggtgcag cgagcaccgc gcggaagctc atcagctcgt cgcgacggaa
     1861 gagatgcagt tccacctcgt cgcccgcacc acgacgggcg agcagatcct ccagcccctt
     1921 ggcgctggag atcctcagcc cgtcgagcgc gatcagcacg tccccggccg agacgcccgc
     1981 cgcctgcgcc ggaccgtcgt cgtacacatt ggcgaccttc gcctcgccac tgcccccgcc
     2041 gagcttcatc ccgagcaccg gcgcggtccc cgccgcctcg gcgcgcaggc tcacgccgaa
     2101 gggacgcagc aggcgcgcca gcggcagatc ctcgcagccg tccaccgcct tctgcagcca
     2161 tttcgccagg cgcgcgccga ggcgttcgcc gcccgcgtcg cgcaccgcgg cgaagatgcc
     2221 atcctccggc acgcccacgc cggtgaggcc gtggcgccgc cacagcagcc gcatcacgtc
     2281 gtccaggctg gccgcaccct cgctgcccgc gcgcagctgc aggtcgagcg cgagcgcgat
     2341 cagcgcaccc ttggcgtagt agctgacgat ggcgttgggt gcgttctcgt cctggcggta
     2401 gtacttggtc cacgcgtcga aggaggactc cgccacgctc tgcttgagcc ggccgctgcc
     2461 gcgcaagacg ttggcgatgg tcttgccgag cagccccagg tagtcatcga tgccgatcac
     2521 gccgctgcgc accagggcga ggtcgtcgta gtacgacgtg aagccctcga aggcccacag
     2581 cagccgggtg tggttctcgc gcgcgagatc gtagggcgtg aacgccgccg gcttgatgcg
     2641 cttgacgttc caggtgtgga aatactcgtg gctgcacagg ccgaggaagc tcttgtagcc
     2701 gtcgggcaga ccctccatgc ccttccacgg cagctcggcg cggctgcaga tcagcgcggt
     2761 cgaggcgcga tgctccagcc cgccgtagcc ctctcccacc accatggtca ggaaggcgta
     2821 gtagtccacc ggcgccggcg tgccgaagag cgcgatctgc cattcgcata tgcggcgcag
     2881 gtcggcgcac aggcgctcga ggtcgcagtc gtggcggccg gtgagcgcga tgtcgtgcgg
     2941 cacgcccgcg gcctcgaagc gtgccagcgt gaaacggccc atctcgaccg ggtggtcgat
     3001 gagctcgtcg tagtccgcgg cgcggaagcg gccgaaccgg caggcctggc ccgggcggcc
     3061 gtgctcgggc ggcagcgcgg tgacgagctt ccagtcgcgc ccgcagtcgc cctcggggcg
     3121 ttcgatggtc accacgcaag gtgcttcggt acgccccgcc accgcgagga agacgctcgt
     3181 gccgttgaag aagccgtgcg tggtgtcgag atgggcggtg cgcaccgaca ggtcccaggc
     3241 atagacctcg taatgcacgc tcagcacgcg cgcgcccggc accgcagccg cgcgccaggt
     3301 gtgcttgtcg agcttctcca gcgcgcaggg ctcgccgtcg gcctcggcac gcaggcgcac
     3361 gatgttgcgc gcgaactcgc ggatcatgta gctgcccggg atccacgccg gcaggctgaa
     3421 gacctgcccc gcggggtcgg gctcggccac cgtgcagctc acctcgaaga ggtgggcgcc
     3481 ggggttcgcc gggcggatgc ggtattcgac cgcgggcggc ggacggtcat ggagcgccga
     3541 cgcggcggcg gtcaagcggg tggaacgggc ggacatcggc aggctccggc ggaactcgtc
     3601 tggaagaggt ccaatcgtaa ccgcatggcc gggcgccggg gaggcgacag aggtcggcag
     3661 cgatctcccg gcgcccaaag gcgaacgccc ggcgtgacgg ccgcgatccg cggatacccg
     3721 tcatccccat gggtcaatta ccgtgcgcat cacgcgaacg actgataggt tccttcgaac
     3781 tcgatgatga tcgaccggag tccgctcaga tcgagctcac tgttgtactc ccggggaatt
     3841 cggattgacc actgacagaa cggcccgatc atttgtcgct gcgcgtccag gaacccgccg
     3901 gtcagcacgg gcgctggtgt cgccgctggt gcgctggtaa cggtgcccgt ctcgggtctt
     3961 gctgccgccg ccgcacctgc cgaaattgtc gtgccgtcgg gggagagcgc acgtgcatcg
     4021 tagcggtact cgtgatgcac tttttgatga ttgacggtga ccggcacgtc gctggcggtc
     4081 acgatcgtct ctacgccggg atgcgtaaac tgaatcacgt gaatgttgtc cttggtcctc
     4141 atcccgagcg cgcacgctcg aatggcggag aggcggacgt tggccattcc cgggaagtgt
     4201 gtatggtcgg tgttggtgtc ctttcgcgcc ggaagaatcg acaccagccc ctcgttgccg
     4261 ttcatcagcg ttgcgaacag cagggggttc cgcgcttttg tgaactcgat tcggcaggag
     4321 ttgcccatgg ggacaaaagc ttgaatggtc gaaagccgct tgttctcctc tttcagtcgt
     4381 ccgacgatga agtcgatcaa cgcggctctg aacgcggtcg agttgatgtt gtcgggccgc
     4441 tgcgagccag ccatcaactg ccgaagcacg tcgacgaaca catccttgtt gctcagggtg
     4501 tgcatcgtga atgcgcggct cgccaggtac agcgtctcca cacactgctc gcgcgcgtga
     4561 cgccacaacg cgctgctgaa cgcggccatc tgcggcaggc tgagaccggc ctgactgacc
     4621 cgccaacttt cggtcttgtt cttggccgac cttatggcat ccgtctgcga tcgcaggtag
     4681 cacaattcgg taagcgcgtc gttgtactcg gcaatcttgg cattccgcgt cgcgaccagt
     4741 tcgatgtagt gcgccagatc gtcgcttaat ttcttcgctg tcggacactt gctgaagaat
     4801 tcggcgcaga tcgcatcaac ctgcctgcgc gtcgccatca ggcggccttg ctcggttccg
     4861 tcggcgctga tcagtccgtc actggcctgt ttcaatccgg tgaagtcggt gacttcattg
     4921 gacaaggaca gcacctgacg gctgagatac gaacgattca ccgtggtgcc gttgtcggtc
     4981 gcgacctcgt tggcagtctt cgagatcaaa tcgccggcct ggcttgcaac gagcggtatc
     5041 gcgaaggctg gcgcctcagg gttgatgaag gccaactg
//
LOCUS       DAOQNN010000233         5070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9951_length_5070_cov_10.624526, whole genome shotgun sequence.
ACCESSION   DAOQNN010000233 DAOQNN010000000
VERSION     DAOQNN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9951_length_5070_cov_10.624526"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /gene="lpdA"
                     /locus_tag="PKV97_11725"
     CDS             <1..1236
                     /gene="lpdA"
                     /locus_tag="PKV97_11725"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNB06592.1"
                     /translation="HTSHLFEEAGHAFEGQGISVGTPKIDVPKMIARKSGIVDQLTGG
                     IKGLFKKNKVTLLNGHGAFVGKADAGWQVQVGEELVTAKQVIVATGSAPRHLPGVPVD
                     NKIVCDNVGALDLDAVPKKLAVIGAGVIGLEMGSVWRRLGAEVTVLEAMPEFLSLADQ
                     DVAKEALKVFTKQGLKFNLGVTIGEVKVGKKGVSIAYKDKDGAEQKLEADRLIVSVGR
                     VPNTQGLNAEAVGLKLNERGQIEVDDHCKTNLPGVWAVGDVVRGPMLAHKAMEEAVMV
                     AELMAGQAGHCNFDTVPWVLYTSPEIAWVGKTEQQLKAAGVAYKVGKIPFLANGRALG
                     MGDSTGFVKMLADATTDRILGVHIIGANASELISEAVVAMEFAGCSEDLARICHAHPT
                     LSEVVHEAALACDKRPLHF"
     gene            1340..2455
                     /gene="zapE"
                     /locus_tag="PKV97_11730"
     CDS             1340..2455
                     /gene="zapE"
                     /locus_tag="PKV97_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNB06593.1"
                     /translation="MPHRILNVPQHGMIDAYEALLKTRGYQSDPAQRAAAQRLQGLYT
                     ELVGFKAARQGSRIKQFLNKPKMPRSVYFWGGVGRGKSFLMDCFYDSVPYRRKRRVHF
                     HAFMQEVQNDLRKFNNAPDPLQKVADRISEHTRLLCFDEFHVSDIADAMILGRLLDAL
                     FDRGVIFVMTSNYPPDGLYPNGLMRINFLPTIEKIKQRFDVIEVDHGTDYRLRTLEKM
                     EIYLVPHDDAAKRKMAEDFRRLAASEGRGGSVEILGRNIDVVRQAPGVIWFDFASLCG
                     GPRSQNDYLEIAREHHTVILSNVPKMLVGNASEARRFTWLIDVLYDHRVKLIMSAAVE
                     AHDLYTEGHNAHEFVRTVSRLIEMRTRDYLAEPHRTE"
     gene            complement(2581..4626)
                     /locus_tag="PKV97_11735"
     CDS             complement(2581..4626)
                     /locus_tag="PKV97_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HNB06594.1"
                     /translation="MKKPDTLDDLPTNLSANEHFQSVVERAVSRRGFLKSGLGLSAVT
                     FLSGSLAACTSDDDTPVAGTPTAGTPPAPAPAAGPLLGFAAVATSSGDAIVVPAGYSA
                     QIFTPWGSPLFSDSPAWRPDGTNTGEEQARQVGDNHDGMSYFPIDGSNEGLLVMNHEY
                     CNYEYLFGAEFMTPWTADKVSKALNAHGVSVLHVKKDGAGRWEVHIGSPYNRRITGKT
                     PMTLTGPAAGDALLRTTADPSGLNVLGTLNNCANGKTLWNTYLTCEENFNGYFATAAS
                     PAPTRSAAFVRYGISAGGSGYRWHEHEDRFDYAKEPNEANRFGWVVEINPFEPGSTPK
                     KRTALGRFKHENAEMRLAADKRVVVYMGDDQANDYIYKFVSDGVFDASRGLANGNLLD
                     AGKLYVAKFDAGAASGDFMGVGEWLLLDKAANPTLAADARFATQAEVLIHARLAADAV
                     GATKMDRPEWITTHPQTGEVYCALTNNSGRTTTDEANPRAQNRYGQIVRWREAGDDAA
                     AMTFEWDLFVLAGNPVAYPDRQDLRSGSANVSADNTFNSPDGIGFDGAGRLWIQTDGN
                     FSNSGDYAGQGNNQMLVADPESKEIRRFLVGPSGCEITGLAFSPDYRTMFINVQHPGE
                     AGSHPRAPDASMRGSLSMDEYLAQNPLAFSQWPEAGGGRPRSATVVITKDDGGVVGS"
     gene            complement(4759..>5070)
                     /locus_tag="PKV97_11740"
     CDS             complement(4759..>5070)
                     /locus_tag="PKV97_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06595.1"
                     /translation="GWGGRCAGTLQAEWRAGGVDILPGQALGDHRIVAAIAAPVTTLE
                     ISALRGRALAVNGKVELSGGQRPRVDLDVGGDPRLLGRLEGMLAELRPTRDGERMRIR
                     F"
BASE COUNT          817 a         1714 c         1713 g          826 t
ORIGIN      
        1 cacacctcgc acctgttcga ggaagccggc cacgccttcg aaggccaggg catcagcgtc
       61 ggcacgccca agatcgacgt gcccaagatg atcgcccgca agtcgggcat cgtcgaccag
      121 ctcaccggcg gcatcaaggg cctgttcaag aagaacaagg tcaccctgct caacggccac
      181 ggcgccttcg tcggcaaggc cgacgccggc tggcaggtcc aggtcggcga agagctcgtc
      241 accgccaagc aggtcatcgt cgccaccggc tcggccccgc gccacctgcc gggcgtgccg
      301 gtcgacaaca agatcgtctg cgacaacgtc ggcgcgctcg acctcgacgc ggtgccgaag
      361 aagctcgccg tcatcggcgc cggcgtcatc ggcctcgaga tgggctcggt gtggcgtcgc
      421 ctcggtgccg aggtcaccgt gctcgaagcc atgcccgagt tcctctcgct cgccgaccag
      481 gacgtggcca aggaagcgct caaggtcttc accaagcagg gcctcaagtt caacctcggc
      541 gtcaccatcg gtgaggtcaa ggtgggcaag aagggcgtca gcatcgccta caaggacaag
      601 gacggcgccg agcagaagct cgaagccgac cgcctgatcg tctccgtcgg ccgcgtgccc
      661 aacacccagg gcctcaacgc cgaagccgtc gggctcaagc tcaacgagcg cggccagatc
      721 gaggtggacg accactgcaa gaccaacctg cccggcgtgt gggcggtggg cgacgtggtg
      781 cgcgggccga tgctcgcgca caaggcgatg gaagaggcgg tcatggtcgc cgagctgatg
      841 gcgggccagg ccggccactg caacttcgac accgtgccct gggtgctgta caccagcccc
      901 gagatcgcct gggtcggcaa gaccgagcag cagttgaagg ccgccggcgt ggcctacaag
      961 gtcggcaaga tccccttcct ggccaacggc cgcgcgctcg gcatgggcga ctcgaccggc
     1021 ttcgtcaaga tgctggccga cgccactacc gaccgcatcc tcggcgtgca catcatcggc
     1081 gccaacgcgt cggagctgat ctccgaagcc gtggtggcga tggagttcgc cggctgctcg
     1141 gaagacctgg cgcgcatctg ccacgcccac ccgaccctgt cggaagtggt gcacgaggcc
     1201 gcgctcgcct gcgacaagcg tccgctgcac ttctgagcga ggcgtgagct gtgaggggtg
     1261 aggtgcgggg gcttccctgc gcccacccct ttttgcgtta acctgcagtc ctccgatcaa
     1321 cgctcctgac cgatttccta tgcctcaccg catcctgaac gttccccagc acgggatgat
     1381 cgacgcctac gaagccctgc tgaagacgcg cggctaccag tccgatcccg cccagcgcgc
     1441 cgcagcacag cgcctgcagg gcctgtacac cgagctcgtc ggcttcaagg cggcgcgcca
     1501 gggcagccgc atcaagcagt tcctgaacaa gcccaagatg ccgcgcagcg tctacttctg
     1561 gggtggcgtg gggcgcggca agagcttcct gatggactgc ttctacgact cggtgccgta
     1621 tcgccgcaag cgccgggtgc acttccacgc cttcatgcag gaggtgcaga acgacctgcg
     1681 caagttcaac aacgcacccg atccgctgca gaaggtcgcc gaccgcatct ccgagcatac
     1741 ccggctgctg tgcttcgacg agttccacgt ctccgacatc gccgacgcga tgatcctggg
     1801 gcggctcctc gacgcgctct tcgaccgcgg cgtgatcttc gtgatgacct cgaactatcc
     1861 gcccgacggc ctctatccca acggcctgat gcgcatcaac ttcctgccca cgatcgagaa
     1921 gatcaagcag cgcttcgacg tcatcgaggt ggatcacggc accgactatc gcctgcgcac
     1981 gctcgagaag atggagatct acctcgtgcc gcacgacgac gcggccaagc gcaagatggc
     2041 cgaggacttc cgccgcctcg ccgcgagcga gggccgcggc ggcagcgtcg agatcctcgg
     2101 gcgcaacatc gacgtggtgc gccaggcccc gggggtgatc tggttcgact tcgccagcct
     2161 gtgcggcggc ccgcgctcgc agaacgacta cctcgagatc gcgcgcgagc accacacggt
     2221 gatcctgtcg aacgtgccca agatgctggt cggcaacgcc tccgaggcac gccgcttcac
     2281 ctggctgatc gacgtgctct acgaccaccg cgtcaagctc atcatgagcg cggcggtcga
     2341 ggcgcacgac ctctacaccg agggccacaa cgcccacgag ttcgtgcgca cggtgagccg
     2401 cctgatcgag atgcgcaccc gcgactacct cgccgagccg caccgcaccg aatagtcggg
     2461 gcgcggcgcc cgtccgccgc gatgggcgtc gcttgggcgc gcgtccggcc cttggacaac
     2521 cagggcggcg cgggcgtgtc ccccgagcgc tcgcgccgcc ctgttcgtcc cgccgtggga
     2581 tcaggagccg accacgcccc cgtcatcctt cgtgatcacg acggtcgccg agcgtgggcg
     2641 accgccgccg gcctcgggcc actgactgaa cgcgagcggg ttctgcgcaa gatactcgtc
     2701 catcgacagg ctcccgcgca tgctcgcgtc cggtgcgcgc ggatgcgaac cggcctcgcc
     2761 gggatgttgc acgttgatga acatggtccg gtagtcgggg gagaatgcaa ggccggtgat
     2821 ctcgcagccc gaaggtccga ccaggaagcg gcggatctcc ttgctctcgg ggtcggcaac
     2881 cagcatctgg ttgttgccct ggcccgcgta gtcgccgcta ttcgagaagt ttccgtcggt
     2941 ctggatccac agccggcccg cgccgtcgaa gccgatgccg tcggggctgt tgaaggtgtt
     3001 gtcggcggat acgttcgcgg aaccggagcg caggtcctgg cggtcggggt aagccaccgg
     3061 gttgcctgcg agcacgaaga gatcccactc gaaagtcatc gcggcggcgt cgtcgccggc
     3121 ctcgcgccag cgcacgatct gtccgtagcg gttctgtgcg cgcgggttcg cctcgtccgt
     3181 cgtggtcctg ccggagttgt tggtcagggc acaatagacc tcgccggttt gcggatgcgt
     3241 ggtgatccac tccgggcgat ccatcttcgt cgcaccgacg gcgtcggcgg cgaggcgggc
     3301 gtggatcagg acttcggcct gggtggcgaa gcgggcgtct gccgccagcg tggggttggc
     3361 cgccttgtcg agcagcagcc attcccccac acccatgaag tcgccgctgg ccgcgccggc
     3421 gtcgaatttg gccacataca gcttgccagc gtcgagcagg ttgccgtttg caagcccacg
     3481 gcttgcgtcg aaaacgccat cggacacgaa cttgtagatg taatcgttgg cctggtcgtc
     3541 gcccatgtag acgaccacgc gcttgtctgc ggcgagcctc atctcggcgt tttcatgctt
     3601 gaagcggccg agcgcggtgc gcttcttcgg cgtggagccg ggctcgaagg gattgatctc
     3661 gacgacccag ccgaagcggt tggcctcgtt gggttccttg gcgtagtcga agcggtcctc
     3721 atgctcgtgc cagcggtagc cggaaccgcc ggcgctgatg ccgtagcgga cgaaggccgc
     3781 gctgcgcgtc ggtgccgggc tcgctgcggt ggcgaaatag ccgttgaagt tctcttcgca
     3841 ggtcaggtag gtgttccaca gcgtcttgcc gttggcgcag ttgttcagcg ttcccaagac
     3901 gttgaggcca ctggggtccg ccgtggtgcg caggagggcg tcgccggcgg cggggccggt
     3961 cagcgtcatc ggcgtcttgc cggtgatccg gcggttgtag ggcgagccga tgtggacttc
     4021 ccagcgcccc gcgccgtcct tcttgacgtg gaggaccgag accccatgcg cgttgagcgc
     4081 cttggagacc ttgtccgccg tccacggcgt catgaattcg gcgccaaaca gatactcgta
     4141 gttgcagtac tcgtggttca tcaccaggag gccttcgttc gatccgtcga tcgggaagta
     4201 gctcatcccg tcgtggttgt cgccgacctg gcgagcctgc tcttcaccgg tgttggtgcc
     4261 gtccggtcgc catgcaggag agtcgctgaa cagcggcgat ccccagggag tgaagatctg
     4321 cgccgaatag cccgcgggga cgacgatcgc atcgccgctc gatgtggcga cggcggcgaa
     4381 gccgaggagc ggccccgcgg caggtgcagg cgcagggggc gtcccggcgg tcggggtgcc
     4441 ggccacgggc gtgtcgtcgt ccgaggtaca ggcagcgagc gagcccgaca ggaaggtgac
     4501 ggccgagagg cccaggccgc tcttcaggaa gccgcggcgg ctgaccgcac gctccacgac
     4561 ggactggaag tgctcgttgg ccgagaggtt ggtcggaagg tcgtcgagcg tatcgggctt
     4621 cttcattgct tcatcactcc ggatgcaggg tcggaaaagc cacgagtttt cctgcgccgg
     4681 attacgcggg cgtgaagggt ttcttacgct tcgacgaggg cgatcccgcg cacgcggcgg
     4741 agtgcggcgg ggggtgattc agaaacggat ccgcatgcgc tcgccgtcgc gtgtcgggcg
     4801 cagctccgcg agcatgcctt ccaggcggcc aagcagccgg ggatcgccgc cgacatcgag
     4861 atcgacgcgc gggcgctgcc cgcccgagag ttccaccttg ccattcacgg cgagcgcgcg
     4921 cccgcgcagg gcgctgatct ccagcgtcgt caccggcgct gcgatcgcgg cgacgatgcg
     4981 atggtcgccg agcgcctggc cgggcaggat gtccacgccg ccggcgcgcc actccgcctg
     5041 cagcgtgccc gcgcagcgtc cgccccagcc
//
LOCUS       DAOQNN010000234         5060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_9987_length_5060_cov_10.946653, whole genome shotgun sequence.
ACCESSION   DAOQNN010000234 DAOQNN010000000
VERSION     DAOQNN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9987_length_5060_cov_10.946653"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PKV97_11745"
     CDS             <1..383
                     /locus_tag="PKV97_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB06596.1"
                     /translation="ERDEAQFCALFAEDGEYHNTPFAVQRGHAQLAAYWQRVKLQEDV
                     QVRYEILATSPAGGIAHWHVTYQVASEELFRIWAQSTGTNLVARKPGDPLPRMVLDGV
                     LKAEFGSGVCTRCAIWWHSMPEPV"
     gene            complement(506..1039)
                     /locus_tag="PKV97_11750"
     CDS             complement(506..1039)
                     /locus_tag="PKV97_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06597.1"
                     /translation="MNAPFLTFLTWPQRWLLLTVLLALSALLLFGRIASLDEFIKARG
                     EGGIMALQFAGSAARAAHLLVAWRGLEDLLRSQTRWDFLFLLVYPAVLSLACAMLAEA
                     RGNPLPTIGIFVAWLALAAIPLDAIENLATLRMIDHGASESLARWITVCATMKFAIVL
                     TAAGYLLVAGIATLFQR"
     gene            1286..1651
                     /locus_tag="PKV97_11755"
     CDS             1286..1651
                     /locus_tag="PKV97_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB06598.1"
                     /translation="MHKTLTAFSAAKTQPSFDFPRPERLVAGNPQRATWAHFERDGMS
                     AGLWGCEPGAWRIAFADDTDEFFHVLSGRIRITDEGGLAREFGPGEACVIPAGFNGVF
                     EVLETVAKHYVFVKRGEMG"
     gene            1729..2913
                     /locus_tag="PKV97_11760"
     CDS             1729..2913
                     /locus_tag="PKV97_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06599.1"
                     /translation="MPARFCSLVLGLCVASAVFAQARPPVPNVVLDHMDELDRRCVAA
                     GGRAGAGRFVVAQDFTGDGRLDYLLSEGDYDCLGRPGLFRPGGAGRVDIFVVDARNAA
                     RRVYSDRLVGYRVLAGKPAKVQIARQGAACGAGSNPRTQCAAQLAWNGHGFGEAVAVS
                     DAGAAPSAAAPVPAAPGVGAAAGSPPAPLVVPPAARSRFLAGCRQAYVSRSADAARWA
                     DEQCVADWGKVTESAEATDALLAALPASAGEAVPLASLRQRLAGVRWAARPRSRELSA
                     SGRLGKFEVSVTGSPAAKTLGMSWMEVGAEPPYDIVGAMRVRGVTLTEIACEEFGAGE
                     WQRVFSGSAPGRAPFRLELGQRIAPFANSNSYYAATIDLSGRPPAPAVSKSACSNPSN
                     LI"
     gene            complement(2935..3981)
                     /locus_tag="PKV97_11765"
     CDS             complement(2935..3981)
                     /locus_tag="PKV97_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB06600.1"
                     /translation="MKILAPSPAELTRGRAATPIAFIRAIVAGYRLRGMDPASALAQA
                     QIPPALLEDPAAHVTAAQMELLSGVAMQELDDEALGWFSRRLPWGSYGMLCRASLTSP
                     TLEVALKRWCRHHRLLTEDIVFELAQRGGMATIHVAEHAGLGELREFCLVSTLRYLLG
                     YACWLVDSRIALGEAAFPFPAPAHADAYAYLFAGPARFSAAAACIRFDARYLALPVRR
                     DEKALQAMLQRALPLTVLQYRRDRLLVHGVAQLLAANPAAAHTAEEVAAQLNLSVRTL
                     HRQLKEEGVSLQRLKNAARREHAVKLLLQSARPVKQIAAAVGFDSEKSFARAFREWTG
                     VAPSAYRSNAEPAC"
     gene            4144..>5060
                     /locus_tag="PKV97_11770"
     CDS             4144..>5060
                     /locus_tag="PKV97_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB06601.1"
                     /translation="MNVPSLNFNLGETIDALRDTLQAFCAAEIAPRAAEIDRVNEFPA
                     DLWKKLGDLGVHGMTVSEEYGGTDMGYLAHIVAMEEISRASASVGLSYGAHSNLCINQ
                     IRRNGTEAQKQKYLPKLISGEHVGALAMSEPNAGSDVVSMKLRADRKGDRFILNGSKM
                     WITNGGDADTLVVYAKTDIAAGPKGITAFIIEKGMKGFSHGTHLDKLGMRGSNTFPLF
                     FDDVEVPAENVLGGEANIGRGVQVLMSGLDYERAVLSGGPLGIMAACMDVVVPYLHER
                     KQFDTPIGEFQLMQGKVADLYSTWSACRA"
BASE COUNT          776 a         1681 c         1851 g          752 t
ORIGIN      
        1 aggaacgcga cgaggcgcag ttctgcgccc tgttcgcgga ggatggcgaa taccacaaca
       61 ccccctttgc ggtgcagcgc ggacatgcgc aactcgccgc ctactggcag cgcgtgaagc
      121 tgcaggagga cgtgcaggtg cgctacgaga tcctcgccac ctcgcccgcc ggcggcatcg
      181 cgcactggca cgtcacctac caggtggcct cggaggagtt gttccggatc tgggcgcaat
      241 ccaccggcac caacctggtc gcgcgcaagc cgggcgaccc gctgccgcgc atggtgctcg
      301 acggcgtgtt gaaggcggag ttcggcagtg gcgtgtgcac gcgctgtgcc atctggtggc
      361 acagcatgcc ggaaccggtg taagggctgc aggggtgtgc gcggaccggg gcgccccgag
      421 tcgctgatcc gggcgtgcgg cgtggtgctg tggtgtcgac ctgcacccgt cccgccgcgg
      481 gggatgcggg atcccggccg ggtcatcagc gctggaagag tgtcgcgatt cccgccacga
      541 gcaggtagcc ggcggcagtg aggacgatgg cgaatttcat cgttgcgcag acggtgatcc
      601 atcgcgccag cgattcgctc gcaccgtggt cgatcatgcg cagcgtggcc agattctcga
      661 tcgcgtcgag cggtatcgcc gcaagtgcga gccaggcgac gaagatcccg atcgtcggca
      721 gagggttgcc tcgcgcctcg gccagcatcg cgcaggcgag cgagagtacc gccgggtaga
      781 ccagcaggaa gaggaagtcc cagcgggtct gcgagcgcaa gagatcctcc aggccgcgcc
      841 aggcgacgag gagatgcgcg gcgcgggccg ccgatccagc gaactggagc gccatgatgc
      901 caccctcgcc cctggccttg atgaactcgt ccaagcttgc gatgcgtccg aacaacaaga
      961 gcgcagaaag ggcgagcagc acggtcaaca gcagccagcg ttgcggccag gtcaggaagg
     1021 tgaggaaggg cgcgttcatt gtgggggcct gggggcctga tacgctcatt ctagacccgc
     1081 cggcgcgcag tccgcggcgg cctcccggcc gggtgaacgt ggtgggggcg tgaggacagg
     1141 gggccgcgcg agcggtcggg catggaggag gacggcggtg cgccttgggc tggagcgtgc
     1201 gttggcgagc tgactggtcg gggctggctc gccaggtcgg gcagcgaggc tagaatgcgg
     1261 cctccactgc ctccccgctg ccgccatgca caagacgctc accgccttct ccgccgcaaa
     1321 gacgcaaccg agcttcgact tcccccgccc cgagcgcctg gtcgcgggca atccgcagcg
     1381 cgccacgtgg gcccacttcg agcgcgacgg catgtccgcc gggctgtggg gctgcgagcc
     1441 gggcgcgtgg cggatcgcct tcgccgacga caccgacgag ttcttccatg tgctctctgg
     1501 acgcatccgc atcaccgacg agggcggtct ggcacgcgag ttcgggccgg gcgaggcgtg
     1561 cgtgatcccg gcaggcttca acggggtgtt cgaggtgctg gagacggtgg cgaagcacta
     1621 cgtgttcgtg aagcgtggcg agatgggttg aggggcgcga tcgggcgggc gccttgcccg
     1681 tggcgggtgc agcgtctagg ctgcagaagc cacccggagg tcgctgccat gcccgcaaga
     1741 ttctgttcgc tcgttcttgg cctctgcgtc gcttcggcgg tcttcgcgca ggcgcgccca
     1801 cccgtcccga acgtggtgct cgatcacatg gacgagctcg accggcgctg cgtcgccgct
     1861 ggcggtcgcg ccggggcagg gcgtttcgtc gtggcgcagg atttcacggg cgatggccgg
     1921 ctcgactatc tgctctccga gggcgattac gactgcctcg gccggccggg tctgttccgc
     1981 ccggggggcg cagggcgggt ggatatcttc gtcgtcgatg cgcgcaacgc ggcgcgccgc
     2041 gtctattccg atcggctcgt cggctatcgg gtgctcgccg gcaagccggc caaggtgcag
     2101 atcgcccgcc agggggcagc ctgcggtgca ggcagcaacc cccgcacgca gtgtgccgcg
     2161 caactggcgt ggaacgggca tggctttggt gaagcggtcg cggtgagtga tgcgggggcg
     2221 gcaccatccg cggccgcgcc tgtgccggcg gcgccaggtg tcggcgcagc ggcgggttcc
     2281 ccgcccgcgc ccctggtggt gccgcccgcg gcgcgttcgc gcttcctggc cggatgccgc
     2341 caggcctatg tttcgcgcag tgcggatgct gcacgctggg ccgacgagca atgtgtggcg
     2401 gactggggca aggtcaccga gagtgcggag gcgaccgatg cactgctggc cgccctgccg
     2461 gcctcggcgg gtgaagcggt gccgctggcg agtctgcgcc agcgcctggc cggcgtgcgc
     2521 tgggcggcca ggccccgctc acgcgagctg tcggcgagcg gtcgcctggg caagttcgag
     2581 gtctcggtga ccggttcgcc cgccgcgaag accctgggaa tgagctggat ggaggtcggc
     2641 gccgagccac cctacgacat cgtcggtgca atgcgcgtgc gcggcgtcac gctcaccgag
     2701 atcgcttgcg aggaattcgg cgccggcgag tggcaacggg tgttctcggg cagcgcgccg
     2761 ggacgggcac cgttccggct cgagctcgga cagcgcatcg cacccttcgc gaattccaat
     2821 tcctattacg ctgcgacgat cgacctctcg gggcggccgc ctgcgccggc ggtgtcgaag
     2881 tcggcgtgct ccaacccgag caacctgatc taggccgtgg atgaaaggcg tccgctagca
     2941 ggcgggctct gcgtttgacc ggtacgcgct tggcgcgacc cccgtccact cccggaacgc
     3001 gcgcgcgaag ctcttctcgc tgtcgaagcc gacggcggct gcgatctgct tcaccggtct
     3061 ggcgctctgc agcagcagct tcaccgcatg ctcccgccgc gccgcgttct tcaggcgctg
     3121 cagcgacacg ccttcttcct tgagctgtcg gtgcagggtg cgcaccgaca ggttgagctg
     3181 tgcggcaacc tcctcggcgg tgtgggcggc ggcggggttg gcggcgagca attgcgcgac
     3241 gccatgcacc agcaggcggt cgcggcggta ctgcagcacg gtgagcggca gcgcacgctg
     3301 cagcatggcc tgcagcgcct tctcgtcgcg gcgcaccggc agggcgaggt agcgcgcgtc
     3361 gaagcggatg caggcggccg cggcggagaa acgcgccggg ccggcgaaga ggtaggcgta
     3421 ggcgtcggcg tgcgccgggg cagggaaggg gaaggcggcc tcgccgagcg cgatgcgcga
     3481 atcgaccagc cagcaggcgt agccgagcag gtagcgcagc gtgctgacga ggcagaactc
     3541 gcgcagctcg ccgagtccgg cgtgctcggc gacgtggatc gtggccatgc cgccgcgttg
     3601 cgcgagctcg aagacgatgt cctcggtcag caggcggtgg tgacggcacc agcgcttgag
     3661 cgccacctcc agcgtcggcg aggtcagtga ggcgcggcag agcatgccgt agctgcccca
     3721 gggcagccgg cgcgagaacc agcccagtgc ctcgtcgtcg agctcctgca tcgccacgcc
     3781 cgagagcagc tccatctgcg cggcggtgac atgtgcggcc ggatcttcca gcagcgcggg
     3841 cgggatctgc gcctgcgcca gcgcgctggc cggatccatg ccccgcagcc gatacccggc
     3901 aacgatcgca cggatgaagg cgatcggcgt ggcggcgcgg ccgcgggtga gttcggcggg
     3961 cgagggggcg aggatcttca tgtgccggaa tgctagccgg cgtggcacga attgcaacct
     4021 cgatggcact cgccgcgcga gcggcccggt taatctcgcc ccatccgcgg gcatgcgccg
     4081 tccataatgg attttccgcg caccgcccca cgcgccagga caacctcgag aaggagacac
     4141 cccatgaacg tgcccagcct gaacttcaac ctcggcgaga ccatcgacgc cctgcgcgac
     4201 accctgcagg ccttctgcgc cgccgagatc gccccgcgcg cggccgagat cgaccgcgtc
     4261 aacgagttcc ccgccgacct gtggaagaag ctcggcgacc tcggcgtgca tggcatgacg
     4321 gtgagcgagg agtacggcgg caccgacatg ggctacctgg cgcacatcgt ggcgatggag
     4381 gagatctcgc gcgcgtccgc ctcggtgggc ctgtcctacg gtgcgcactc caacctgtgc
     4441 atcaaccaga tccgccgcaa cggcaccgag gcgcagaagc agaagtacct gcccaagctg
     4501 atctcgggcg agcacgtggg cgcgctggcg atgtccgaac ccaacgccgg ctccgacgtg
     4561 gtgagcatga agctgcgcgc cgaccgcaag ggtgaccgct tcatcctcaa cggcagcaag
     4621 atgtggatca ccaacggcgg cgacgccgac accctggtgg tctatgccaa gaccgacatc
     4681 gccgccggcc ccaagggcat caccgccttc atcatcgaga agggcatgaa ggggttcagc
     4741 cacggcaccc acctcgacaa gctcggcatg cgcggctcca acaccttccc gctgttcttc
     4801 gacgacgtcg aggtgccggc ggagaacgtg ctcggcggcg aggccaacat cggtcgcggc
     4861 gtgcaggtgc tgatgagcgg cctggactac gagcgcgcgg tgctctccgg cggcccgctc
     4921 ggcatcatgg cggcgtgcat ggacgtggtg gtgccctacc tgcacgagcg caagcagttc
     4981 gacaccccga tcggcgaatt ccagctcatg cagggcaagg tcgccgacct gtactccacc
     5041 tggagcgcct gccgcgccta
//
LOCUS       DAOQNN010000235         5048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10024_length_5048_cov_11.136792, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000235 DAOQNN010000000
VERSION     DAOQNN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10024_length_5048_cov_11.136792"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PKV97_11775"
     CDS             <1..698
                     /locus_tag="PKV97_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB06602.1"
                     /translation="IRKGEVYGLIGPNGAGKTTFFNVLTGAYTPDGGDFVFNGSELPS
                     GKPHKVVGRGIARTFQNIRLFRDLTALENVMAGHHIRTRAGIWGVLTQNRFTREEERL
                     TTERAYELLRYVGIERFADVVSKNLSYGDQRRLEIARALATEPQLLALDEPAAGMNAT
                     ETGQLKQLIEQIRNDGITVMLIEHDVKLVMGLCDRVAVLDFGSKIAEDVPAVVQKDEA
                     VIAAYLGGGKHAN"
     gene            688..1434
                     /locus_tag="PKV97_11780"
     CDS             688..1434
                     /locus_tag="PKV97_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB06603.1"
                     /translation="MQTDPRLEPSTPLLRLQELKIAYGGIQAVKGIALELHAGETVCL
                     IGANGAGKTTTLNAIAGVLPIAGGDIHYKGERINALPAHRRLRAGIALVPEGRGIFTR
                     LTVEENLRMGAYTRKDTAAIEADLERVYTMLPRVKERLAQVAGTLSGGEQQMVAIGRA
                     LLSRPRLLLLDEPSMGLAPLIVEKIFEVVQSVAREGVTILLVEQNANLALEFAQRGYV
                     MESGRITLTGSGSALLQDPKVRAAYLGEEA"
     gene            1645..2664
                     /locus_tag="PKV97_11785"
     CDS             1645..2664
                     /locus_tag="PKV97_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNB06604.1"
                     /translation="MQNLKYFTWSIGFTVFGLVGGYLIGGWPAAFIVAVLAVLETSLS
                     FDNAVVNATVLRHMDEKWRQRFLLWGILIAVFGMRIVFPLAIVGVVASLGPVEVVDLA
                     LFNPDEYARLLTSAHHEIAAFGGAFLMMVFLKFFIDENKDTHWLAPIESPLTKLGRLE
                     AAQILLTLLAILATSAWIDGADKRMEFIVAGVWGLIVYIAADGLGAIMGGEEGEGGKM
                     VAKTGFAGFMYLELLDASFSFDGVIGAFALTTSLPIIAIGLGVGAMFVRSFTLMLVYR
                     GTLEAYRYLEHGAFWAIGALAAIMFVGVHMHIPETFTGLIGAVLIGAAFWSSLRHNRL
                     SVGGG"
     gene            2935..4836
                     /locus_tag="PKV97_11790"
     CDS             2935..4836
                     /locus_tag="PKV97_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor I family protein"
                     /protein_id="HNB06605.1"
                     /translation="MSHRLRHALETFSLSLLAAATLAACTGDDDTPPPPPATETAPAP
                     QAPAPERTPISLRLERIGGYATNLFGKSAAEIPAYDPASKRAFVVNADAGAIDVLDLA
                     NPATPISVGRLDSQALLAGSEINSVAVRDGVVAVAIQASPKTDKGRVALYRAADLALL
                     GSVEVGALPDMLTFTPDGKTVLVANEGEPNDDYSIDPEGSISVIDVSTPTAPVARSAG
                     FTAFNGQEKALRAQGVRIYGPGASAAQDLEPEYIAVSADGATAWATLQENNALARIDV
                     AKATVEAILPLGDKDHGVDANAFDPSDTDKKAVIQAWPGVRGLYLPDAIASYTHQGRT
                     LLVTANEGDARTWGEGDDAYWDGDASKGFVEEFRVKHLTNTRGWTGRKGDDLPPQLDA
                     LADGGLLDPTVFAYCGAVAGSPGQCRDDDQLGRLNITWTMGYRQDADGKPILFDTAGN
                     ENPAGTRLMYDTLYAFGGRSFSIRDADGKLVWDSGAEIERFLASDECKLGSARNIPCA
                     TWFNANHEEGDTLDNRSDNKGAEPEGVALGRIGDKTFAFVGLERMGGVLVYDITDPQA
                     PTRVDYLNPREDWSTKDPSTVLASVGDLGPEGLHFIPAKDSPNAKPLLLVGNEVSGTT
                     AVYQLNLSY"
     gene            complement(4820..>5048)
                     /locus_tag="PKV97_11795"
     CDS             complement(4820..>5048)
                     /locus_tag="PKV97_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06606.1"
                     /translation="RMHARFDAALDARVVWVEYGWWQPGAAGAADGYDARSEAGANYS
                     RLVSDRRVDPVVGSQPLRSGLCEIEPLSSSG"
BASE COUNT          817 a         1870 c         1646 g          715 t
ORIGIN      
        1 ccatccgcaa gggcgaggtc tacggcctga tcggccccaa cggcgccggc aagaccacct
       61 tcttcaacgt cctcaccggt gcctacaccc ccgacggcgg cgacttcgtc ttcaacggca
      121 gcgagctgcc cagcggcaag ccgcacaagg tggtcggccg cggcatcgcc cgcaccttcc
      181 agaacatccg cctgttccgc gacctcaccg cgctcgagaa cgtcatggcc ggccaccaca
      241 tccgcacccg ggcgggcata tggggcgtgc tgacgcagaa ccgcttcacc cgcgaggagg
      301 aacgcctgac caccgagcgc gcctacgagc tgctgcgcta cgtcggcatc gagcgcttcg
      361 cagacgtggt gtcgaagaac ctctcctacg gcgaccagcg ccgcctggag atcgcgcgcg
      421 cgctcgccac cgagccgcag ctgctcgcgc tcgacgaacc ggccgccggc atgaacgcca
      481 ccgaaaccgg ccagctcaag caactgatcg agcagatccg caacgacggc atcaccgtga
      541 tgctgatcga gcacgacgtg aagctggtga tgggcctgtg cgaccgcgtc gcggtgctcg
      601 acttcggcag caagatcgcc gaggacgtgc ccgcagtggt gcagaaggac gaagcggtga
      661 tcgccgccta tctgggcgga ggcaagcatg caaactgatc cccggctcga gccgagcacg
      721 ccgctgctgc gcctgcagga gctgaagatc gcctatggtg gcatccaggc ggtcaagggc
      781 atcgcgctcg agctccatgc cggcgagacc gtgtgcctga tcggcgccaa tggcgccggg
      841 aagacgacca cgctcaacgc catcgccggc gtactgccga tcgccggcgg cgacatccac
      901 tacaagggcg agcgcatcaa cgccctgccc gcacatcgcc gtctgcgcgc aggcatcgcg
      961 ctggttcccg aggggcgcgg catcttcacc cgcctgacgg tggaggagaa cctgcgcatg
     1021 ggcgcgtaca cgcgcaagga cacggccgcc atcgaggccg acctcgagcg cgtctacacc
     1081 atgctgccgc gcgtcaagga acgcctggcc caggtcgccg gcacgctgtc gggcggcgag
     1141 cagcagatgg tggcgatcgg ccgcgccctg ctgtcgcgcc cgcgcctgct gctgctcgac
     1201 gagccgtcca tgggcctggc accgctgatc gtggagaaga tcttcgaggt cgtgcagtcg
     1261 gtggcgcgcg agggtgtcac catcctgctc gtcgagcaga acgccaacct cgcgctcgag
     1321 ttcgcgcagc gcggctacgt gatggagtcg gggcggatca ccctcaccgg cagtggcagc
     1381 gccttgttgc aggatccgaa ggtgcgcgcg gcgtacctgg gcgaggaggc ctaggacgag
     1441 gcgcgtcgcg attccggcct ctccggaggc gccggcgcga ctcgaccgtc cgatcgcgac
     1501 tggcgtcgct cctggggcac ggctccggag gccatgtcgg ttcgctcgcc cgagcgcaag
     1561 tcggcggagc acgagccggc caccgcactc cgcagcgggg agggaaagcg ctaacattgc
     1621 gcactttttt gcggccctcc ccccatgcag aatctgaagt acttcacctg gtcgatcggc
     1681 tttaccgtct tcgggctcgt cggcggctac ctgatcggcg gctggccggc cgccttcatc
     1741 gtcgccgtgc tcgcggtgct ggagacctcg ctgtccttcg acaacgcggt ggtcaacgcc
     1801 accgtgctgc gccacatgga cgagaagtgg cgccagcgct tcctgctgtg gggcatcctg
     1861 atcgcggtgt tcggcatgcg catcgtgttc ccgctcgcca tcgtcggcgt ggtcgccagc
     1921 ctggggccgg tcgaggtggt cgatctcgcg ctcttcaacc ccgacgaata cgcccgcctg
     1981 ctcacctcgg cgcatcacga gatcgcggcc ttcggcggcg ccttcctgat gatggtcttc
     2041 ctgaagttct tcatcgacga gaacaaggac acccactggc tggcgccgat cgagagcccg
     2101 ctgaccaagc tcggccgcct ggaggccgcg cagatcctgc tgaccctgct cgccatcctc
     2161 gccacctcgg cgtggatcga cggtgcggac aagcgcatgg agttcatcgt cgccggggtg
     2221 tgggggctga tcgtctatat cgccgccgac ggcctgggcg cgatcatggg cggcgaggag
     2281 ggcgaaggcg gcaagatggt cgccaagacc ggcttcgccg gcttcatgta cctggagctg
     2341 ctggacgcgt ccttcagctt cgacggcgtc atcggcgcct tcgcgctcac caccagcctg
     2401 ccgatcatcg ccatcggcct gggcgtgggg gcgatgttcg tgcgctcgtt tacgctgatg
     2461 ctggtgtatc gcggcacgct cgaggcctac cgctacctgg agcacggcgc cttctgggcc
     2521 atcggtgcgc tggcggcgat catgttcgtg ggagtgcaca tgcacatccc ggagaccttc
     2581 accggcctga tcggcgcggt gctgatcggc gcggcgttct ggagctcgct gcggcacaac
     2641 cggctgagcg taggcggggg ctgaagcgca gaatcgcgac tcgcgtcgct cccacaagac
     2701 catcgcggct ccgcggcggt ggaagcaccg catccccgat ccggagcgaa ccacctcgcc
     2761 cgggtcgcat gtgggagcga cgcaagtcgc gattgcggct gcgcctgcaa acgattgcgg
     2821 cgacacctgc gcacgatggc ggccggtcgg acggtctgcg cgcagcctcc tgtaacaccc
     2881 cggtcacgcc gtgctcatag attcgggggc ctttgcccac cgccccccga caccatgagc
     2941 caccgcctgc gccacgccct cgagaccttc tccctcagcc tcctcgccgc cgccacgctg
     3001 gctgcgtgca ccggcgacga cgacactcca ccgccgccgc ctgcaaccga aaccgccccg
     3061 gccccgcagg cccccgctcc cgagcgcacc ccgatctcgc tgcgcctgga gcgcatcggc
     3121 ggctacgcga ccaatctctt cggcaagagc gccgcggaga tccccgccta cgaccccgcc
     3181 agcaagcgcg ccttcgtggt caacgcggat gcgggcgcga tcgacgtgct cgacctcgcc
     3241 aaccccgcaa cgccgatctc cgtcggccgg ctcgactcgc aggccctgct cgccggctcc
     3301 gagatcaaca gcgtcgccgt gcgcgatggc gtggtcgcgg tcgcgatcca ggccagcccc
     3361 aagaccgaca agggccgggt cgcgctctac cgcgcggccg accttgcgct gctgggcagc
     3421 gtcgaggtcg gcgccctgcc cgacatgctc acctttaccc ccgacggcaa gaccgtgctg
     3481 gtcgccaacg aaggcgagcc gaacgacgac tactcgatcg accccgaggg ctcgatcagc
     3541 gtgatcgacg tctccacccc cacggccccg gtcgcccgca gcgccggctt caccgccttc
     3601 aacggccagg agaaggcgtt gcgcgcccag ggcgtgcgca tctacggccc cggcgcgagc
     3661 gccgcgcagg acctcgagcc cgagtacatc gccgtctcgg ccgacggcgc caccgcgtgg
     3721 gccacgctgc aggagaacaa cgcgctcgcc cgcatcgacg tggccaaggc cacggtggag
     3781 gcgatcctgc cgctcggcga caaggaccac ggtgtggatg cgaacgcgtt cgatccctcc
     3841 gacaccgaca agaaggccgt catccaggcc tggccgggcg tgcgcggcct ctacctgccc
     3901 gacgcgatcg cctcgtacac ccaccagggc cgcaccctgc tggtcaccgc caacgagggt
     3961 gacgcccgta cctggggcga aggcgacgac gcctactggg acggcgacgc cagcaagggc
     4021 ttcgtcgagg agttccgcgt caagcacctc accaacacca ggggctggac cggccgcaag
     4081 ggcgacgacc tgccgccgca gctcgacgcg ctcgccgacg gcggcctgct cgacccgacg
     4141 gtcttcgcct actgcggcgc ggtcgccggc agccccggcc agtgccgcga cgacgaccag
     4201 ctcggccgcc tcaacatcac ctggaccatg ggctatcgcc aggacgccga cggcaagccg
     4261 atcctgttcg acaccgccgg caacgagaac ccggccggca cccgcctgat gtacgacacg
     4321 ctctacgcct tcggcggacg ctcgttctcg atccgcgacg cggacggcaa gctggtgtgg
     4381 gactcgggcg ccgagatcga gcgcttcctc gccagcgacg agtgcaagct cggcagcgcg
     4441 cgcaacatcc cctgcgcgac ctggttcaac gccaaccacg aggaaggcga cacgctggac
     4501 aatcgcagcg acaacaaggg tgcggaaccc gaaggcgtcg cgctcggccg catcggcgac
     4561 aagaccttcg ccttcgtcgg cctcgagcgc atgggcggcg tgctggtcta tgacatcacc
     4621 gacccgcagg cgccgacccg cgtggactac ctcaacccgc gcgaggactg gagcaccaaa
     4681 gacccctcga ccgtgctggc gagcgtcggc gacctcggcc ccgaaggcct gcacttcatc
     4741 cccgccaagg actcgcccaa cgccaagccg ctgctgctgg tcggcaacga ggtcagcggc
     4801 accacggcgg tgtaccagct caacctgagc tactgagcgg ctcgatctcg cacagccccg
     4861 agcgcagggg ctgcgagccg acgaccggat cgacgcggcg gtcggagacg aggcggctgt
     4921 agttcgcccc cgcctccgag cgcgcgtcgt agccgtcggc cgcccccgcc gcgccgggct
     4981 gccaccagcc gtattccacc cacaccacgc gcgcatcgag cgccgcatcg aagcgcgcat
     5041 gcatgcgg
//
LOCUS       DAOQNN010000236         5047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10027_length_5047_cov_8.870793, whole genome shotgun sequence.
ACCESSION   DAOQNN010000236 DAOQNN010000000
VERSION     DAOQNN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10027_length_5047_cov_8.870793"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKV97_11800"
     CDS             <1..329
                     /locus_tag="PKV97_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNB06607.1"
                     /translation="PRRLAERGFNQAVELARGFARARDLPLGLAVVRKLRDLPAQAGL
                     DREARLRSPRGAFECVEDLSGRRVIVVDDVMTTGATLDELARCLKGRGASWVGNLVVA
                     RTPSPS"
     gene            467..943
                     /locus_tag="PKV97_11805"
     CDS             467..943
                     /locus_tag="PKV97_11805"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNB06608.1"
                     /translation="MFEIVLYQPEIPPNTGNVIRLAANTGCRLHLVAPLGFDLSDKQL
                     ARAGLDYHDLTCLTVHPDWAHCREVLAGRRMFAMSTRASRRHDRVDFQPGDALLFGPE
                     SRGLPEEILREFAPERRVRIPMRPTNRSVNLSNAVAVVVYEGWRQHDFAGAVAQGD"
     gene            complement(965..1957)
                     /locus_tag="PKV97_11810"
     CDS             complement(965..1957)
                     /locus_tag="PKV97_11810"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNB06609.1"
                     /translation="MSRRRIAVFGAGAWGTALAQAFSASHEVVLWGRDRGQLEEIARI
                     HENTRYLRGVRLRDTLAVEADFARAAAQAELHLVVTPLAGLRTTVRELRRLAPGRALL
                     WACKGFEAGTGKLPHEIVAEECGAGSASGVLTGPSFAAEVARGLPAAIALAAHDADFA
                     RHWVGVLHQPRLRIYANTDLVGCEVGGAVKNVMAIAAGVSDGMGFGLNARAALITRGL
                     AEIARLARALGARGETLMGLAGMGDLILTCTGDLSRNRRVGLALAEGKGLADILRQLG
                     HVAEGVSTAREVVGLARRHGVEMPICEAIDALLHHGLDARGAVERLLARDPRAE"
     gene            complement(1954..2451)
                     /locus_tag="PKV97_11815"
     CDS             complement(1954..2451)
                     /locus_tag="PKV97_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNB06610.1"
                     /translation="MKRARRPSPQIPSAPLGRALLMLAVFAPLGGAALPVAAQAIEYR
                     SVAASTLLREQPAPDAEALFRLRPGTPVEIVVREDGWMRVRDPAGGFGWVEDGALVTR
                     RTVIVTAERAIVRRAAQETAAPAFEATRNVVLELLEPASEGWARVRHVEGFEGYVHAS
                     EVWGL"
     gene            complement(2462..2926)
                     /gene="secB"
                     /locus_tag="PKV97_11820"
     CDS             complement(2462..2926)
                     /gene="secB"
                     /locus_tag="PKV97_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNB06611.1"
                     /translation="MSENTQPVFSIEKLYVKDLSVEVPGAPQIFLERETPQVNVQLRT
                     EGQVVEEGVYEVTLTVTVNARIGEERNLFLVEVAQAGIFQIRNLPEGELEPVMMIGCP
                     NILFPYAREAVSDAVTRAGFQPVLLSPVNFESLYQAQRQQAIEQANGEVPIQ"
     gene            complement(3053..3322)
                     /gene="grxC"
                     /locus_tag="PKV97_11825"
     CDS             complement(3053..3322)
                     /gene="grxC"
                     /locus_tag="PKV97_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNB06612.1"
                     /translation="MQPPIRMYATATCPYCIRAEQLLLDKGVVGLDKIRVDLDPSRRE
                     EMMKLSGRRTVPQIFIGDYHVGGCDDLFALERGGRLAALLRGESV"
     gene            complement(3325..3741)
                     /locus_tag="PKV97_11830"
     CDS             complement(3325..3741)
                     /locus_tag="PKV97_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB06613.1"
                     /translation="MEFLQHNWYWAALAAASGTWLMVELVRSRGDDTQLSPVEATLLV
                     NREDAIVIDVREQGEYAQGHIPNARHIPAGEIERRGKEMEKWKDHPVILCCTTGARSN
                     SAAGALRKAGFNRIYNLRGGMMEWQKAGQPVSRKRK"
     gene            complement(3774..4103)
                     /locus_tag="PKV97_11835"
     CDS             complement(3774..4103)
                     /locus_tag="PKV97_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB06614.1"
                     /translation="MDKDAITTLIDKHEHIETAARALKAIAHPLRLKILCVLGSGEEC
                     VQDIVDAVGTSQSNISQHLAILRDKGVLQTRKDANRVYYRVGDERTLQLIVLMREVFC
                     ADDDAER"
     gene            4311..>5047
                     /locus_tag="PKV97_11840"
     CDS             4311..>5047
                     /locus_tag="PKV97_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HNB06615.1"
                     /translation="MSPRSLKSPIAGACIAALAALLVVPGPAFAQAGGEIAERRSDLD
                     ELKQRIRELQEEMAKAEADRTSAQKALVDSERSVSRAVRELARIEGERRNAEKRLAVL
                     EGEQRETAARIDARRGELADWVRRHYVHGAADGVAPLLSARDPNQLARDAHYLEHLGR
                     ARLELIEGLRTDLSETRRRADEISARRDRLATLESEQRDRQQQLQTEQSRRKAALAEV
                     SRELQAKKSEVDALQQDEQRLGKLIETL"
BASE COUNT          713 a         1754 c         1829 g          751 t
ORIGIN      
        1 atccgcgcag gctcgccgag cgcggcttca accaggcggt ggagctggcg cgcggattcg
       61 cgcgtgcgcg cgacctgccg ctcgggctcg ccgtcgtgcg caagctgcgc gacctgccgg
      121 cgcaggccgg gctcgaccgc gaggcccgcc tgcgcagccc gcgcggcgcc ttcgagtgcg
      181 tggaggacct gagcgggcgc agggtgatcg tggtcgacga cgtgatgacc accggcgcca
      241 cgctcgacga gcttgcgcgc tgcctcaagg ggcgaggggc gagctgggtc ggcaacctgg
      301 tcgtggcgcg cacgccttcg ccctcttgag cccgcggtgg tggcggcctg ctagactcgc
      361 cggccggcgg ctccgctgca ctcgcttcgg cttgcggagc ggtcttgccg cagccgattt
      421 ccgcccggcc atgcacctgc acccggccaa ccttggctat ccagcgatgt tcgagatcgt
      481 cctctaccag cccgagatcc cgcccaacac cggcaacgtg atccgcctcg ccgccaacac
      541 cggctgccgc ctccatctgg tggcgccgct cggcttcgac ctgtcggaca agcagctcgc
      601 ccgcgccggc ctggactacc acgatctcac ctgcctgacc gtgcatcccg actgggcgca
      661 ctgccgcgag gtgctcgccg ggcggcgcat gttcgccatg agcacgcgtg cgagccggcg
      721 tcacgaccgt gtggatttcc agcctggcga tgcgctgctc ttcggtcccg agagccgcgg
      781 cctgcccgag gagatcctgc gcgagttcgc gcccgagcgg cgggtgcgca tcccgatgcg
      841 cccgaccaac cgcagcgtca atctgtcgaa cgcggtggcg gtggtggtgt acgagggctg
      901 gcgccagcac gactttgccg gcgccgtcgc gcagggcgac tgagccgagc cgcgccgcgc
      961 gcgctcactc cgcgcgcggg tcgcgtgcga gcaggcgctc cacggcgccg cgcgcgtcga
     1021 gtccgtgatg gaggagagcg tcgatggctt cgcagatcgg catctcgacg ccatggcggc
     1081 gggcgaggcc gaccacctcg cgtgcggtcg acacgccctc ggcaacgtgg ccgagctggc
     1141 gcaggatgtc ggcgaggccc ttgccttcgg ccagcgccag gccgacgcgg cggttgcgcg
     1201 agaggtcgcc ggtgcaggtg agaatcaggt cgcccatgcc ggccaggccc atcagggtct
     1261 cgccgcgcgc gccgagcgca cgcgcgaggc gggcgatctc ggccaagccg cgggtgatca
     1321 gggcggcgcg cgcattgagt ccgaagccca tgccgtcgga gacgccggcg gcgatcgcca
     1381 tgacgttctt gaccgcgccg ccgacctcgc agcccaccag atcggtgttg gcgtagatgc
     1441 gcaggcgggg ctggtgcagc acgcccaccc agtggcgggc gaaatcggcg tcgtgtgccg
     1501 ccagcgcgat cgccgcgggc aggccgcgcg cgacctcggc ggcgaagctc gggccggtga
     1561 gtacgccact cgcggacccg gcgccgcact cctcggcgac gatctcgtgc ggcagcttgc
     1621 cggtgccggc ctcgaagccc ttgcaggccc acagcagcgc acgcccggga gcgagccgac
     1681 gcagctcgcg caccgtggtg cgcaggccgg cgaggggggt gacgaccaga tgcagctcgg
     1741 cctgcgctgc ggcgcgcgcg aagtcggcct cgaccgcgag cgtgtcgcgc aggcggacac
     1801 cgcgcaggta gcgggtgttc tcgtggatac gggcgatctc ttcgagctgg ccgcggtcgc
     1861 ggccccacag gaccacctcg tggctggcgg aaaaggcttg ggcgagcgcg gtgccccagg
     1921 cgcccgcgcc gaacacggcg atgcggcggc ggctcacagt ccccatacct cgctggcgtg
     1981 cacgtagcct tcgaagccct cgacgtggcg cacgcgcgcc cagccctcgc tggcgggttc
     2041 gagcagttcg aggacgacgt tgcgcgtggc ttcgaaggcg ggcgcggcgg tctcctgcgc
     2101 ggcgcggcgc acgatcgcgc gctccgcggt gacgatgacc gtcctgcgcg tgacgagggc
     2161 gccgtcctcg acccagccga acccgccggc agggtcgcgc acgcgcatcc agccgtcctc
     2221 gcgcacgacg atctcgaccg gtgtgcccgg gcgcaggcgg aacagcgcct cggcatccgg
     2281 cgccggctgc tcgcgcagca gggtcgaagc cgcgaccgag cggtattcga tcgcctgcgc
     2341 ggcgaccggc aaggccgcgc cgcccagggg ggcgaacacg gcgagcatca ggagcgcgcg
     2401 gccgagcggc gccgaaggta tctgcgggga cggacggcgc gcgcgcttca tgcgggacgc
     2461 ctcactggat gggaacttcg ccgttcgcct gctcgatggc ctgctggcgt tgcgcctggt
     2521 agagcgattc gaaattgacc ggcgaaagca gcaccggctg gaaaccggca cgggtgaccg
     2581 cgtccgacac cgcctcgcgg gcgtagggga agaggatgtt ggggcagccg atcatcatca
     2641 ccggctccag ctcaccctcg gggaggttgc ggatctggaa gatgccggcc tgcgcgacct
     2701 cgaccaggaa gaggttgcgt tcctcgccga tgcgggcgtt gacggtgacg gtcagggtga
     2761 cttcgtacac gccttcctcg accacctggc cttcggtacg cagttgcacg ttcacctgcg
     2821 gggtctcgcg ctcgaggaag atctgcgggg cacccggcac ctcgaccgag aggtccttga
     2881 cgtagagttt ttcgatcgag aagacgggtt gggtgttttc gctcatgaat cgggttccgg
     2941 tttgggtggg tacggccgcg cctgcggccg cggggaaaaa ggatgctcgg cggtggagcg
     3001 tcggagatgg tggggcgggg gcgcctcgcg tgccgcgggt acgaacccgc gctcagacgc
     3061 tctcgccgcg cagcagcgca gcgagcctgc cgccgcgctc gagggcgaag agatcgtcgc
     3121 agccgcccac atggtagtcg ccgatgaaga tctgcggaac ggtgcggcgt ccgctcagct
     3181 tcatcatctc ctcgcgacgc gaggggtcga gatcgacgcg gatcttgtcc agcccgacca
     3241 cgcccttgtc gagcagcaat tgctcggcgc ggatgcagta ggggcaggtg gcggtcgcgt
     3301 acatgcggat cggaggctgc atgctcactt cctcttgcgg ctgacgggct gccccgcctt
     3361 ctgccactcc atcatgccgc cgcgcaggtt gtagatgcgg ttgaatccgg ccttgcgcag
     3421 cgcccctgcg gcgctgttcg agcgcgcgcc ggtggtgcag caaaggatga ccgggtggtc
     3481 cttccacttt tccatctcct tgccgcggcg ctcgatctcg ccggcgggga tgtggcgcgc
     3541 gttggggatg tggccctggg cgtactcgcc ctgctcgcgc acgtcgatga cgatcgcgtc
     3601 ctcgcggttg acgaggaggg tcgcctccac gggcgagagc tgggtgtcgt cgccgcggct
     3661 gcgcacgagt tcgaccatca gccaggtgcc cgaggcggcg gcgagcgccg cccagtacca
     3721 gttgtgttgc aggaattcca cgaaggggat gctccggttg cggggttggg gggtcagcgt
     3781 tcggcgtcgt cgtcggcgca gaagacctcg cgcatcagca cgatcagctg cagggtgcgc
     3841 tcgtcgccga cgcggtaata gacgcggttg gcgtccttgc gcgtctgcag cacacccttg
     3901 tcgcgcagga tggcgagatg ctgggagatg ttgctctgcg aggttcccac cgcgtcgacg
     3961 atgtcctgca cgcattcttc gccgctgccg agcacacaga ggatcttcag gcgcagggga
     4021 tgcgcgatcg ccttgagcgc gcgggcggcg gtctcgatgt gttcgtgctt gtcgatcagg
     4081 gtggtgattg cgtctttgtc cacgatgggg ccggcagaac caaagcgcta gtataaaata
     4141 acgcgttgtc ccgatgcatg agagatctcg cgtccgagcg gccgcgtgcc ggtcacgagc
     4201 cgccgcctcg cgcacccttc accgcatcgg gtcccttgtc cgactgccct ccgtcctcgc
     4261 ccccgtccag cattgctcgc ccctcgcaac tctccgcgga tcccaccccg ttgtcgccca
     4321 gaagcctgaa gtcgccgatc gcaggcgcgt gcatcgccgc gctcgccgcc ctccttgtcg
     4381 tgcccggccc ggccttcgcc caggccggcg gcgagatcgc cgagcggcgc tccgacctcg
     4441 acgagctcaa gcagcgtatc cgcgagttgc aggaggagat ggcgaaggcg gaggccgatc
     4501 gcaccagcgc gcagaaggcg ctggtcgact ccgagcgttc ggtgtcgcgc gccgtacgcg
     4561 agctcgcccg catcgagggc gagcgcagga atgccgagaa gcggctcgcg gtgctcgaag
     4621 gcgagcagcg cgagaccgcc gcgcgcatcg acgcccgccg cggtgagctc gccgactggg
     4681 tgcgtcgcca ctacgtgcat ggcgcggccg acggtgtggc gccgctgctg tcggcacgcg
     4741 accccaacca gctcgcgcgc gacgcgcact acctggagca ccttggtcgc gcccgcctgg
     4801 agctcatcga aggcctgcgc accgacctct ccgagacccg gcgccgcgcc gacgagatca
     4861 gcgcccgccg cgatcgtctc gccacgctcg agagcgagca gcgcgaccgc cagcagcagc
     4921 tgcagaccga gcagtcgcgg cgcaaggccg cgctcgccga ggtgagccgc gagctgcagg
     4981 cgaagaagag cgaggtcgat gcgctgcagc aggacgaaca gcgtctcggc aagctcatcg
     5041 agaccct
//
LOCUS       DAOQNN010000237         5036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10065_length_5036_cov_7.327645, whole genome shotgun sequence.
ACCESSION   DAOQNN010000237 DAOQNN010000000
VERSION     DAOQNN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10065_length_5036_cov_7.327645"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PKV97_11845"
     CDS             complement(<1..892)
                     /locus_tag="PKV97_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB06616.1"
                     /translation="MRIQKSVFALTTALLCLSLAACSERPAPVAAVEATRPAPILTVG
                     RRTADEALRLPGRVRAARRAELSFDVPGFVDSFSLEEGRRVKAGEVVARLDDRIYRSR
                     LDAARAEFERARNDLARYQRLWESEQAVARAEVDDRRARLEAARTSLAAAERDLADTA
                     IKAPFEGVITRRRIEPFTNVQAKQPIAELQDLRALEVVVNVPERLVRRMRPQQAAVAF
                     LEGERSVEGDRAAPGQALRLELQSFAAEADALTQTYAVVLAVRSVPAGVNLLPGMAVS
                     LEPAADAGGAPAADAAITVPL"
     gene            complement(1037..1246)
                     /locus_tag="PKV97_11850"
     CDS             complement(1037..1246)
                     /locus_tag="PKV97_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06617.1"
                     /translation="MRTKAFPLLAVLGCLLLASCGGDTYVKVQGTTTISKGQELADLQ
                     RALDEGAIDRGDYDLLRKRILDRPN"
     gene            complement(1250..1450)
                     /locus_tag="PKV97_11855"
     CDS             complement(1250..1450)
                     /locus_tag="PKV97_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06618.1"
                     /translation="MNTRLALFAGLIGLSLTGCGGHEVVRPTVDVSIGQQLIDLKRAR
                     EAGALSDREYERQRERLIDSVE"
     gene            complement(1646..2143)
                     /locus_tag="PKV97_11860"
     CDS             complement(1646..2143)
                     /locus_tag="PKV97_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06619.1"
                     /translation="MTIRSATRYLAGVVLCAGLALGAHAGTPEEDATLGLIREQFQDV
                     DLRRVIEVSASMKLDEQGAKRFWPIYRAYLAEIVALRDRQIDTLAEYARQLDAGWIDD
                     PTARTSLARSLALEQARVDARHHMIRKAGEVITPIQTLRLYQLELAMDASIGSRVLQQ
                     IPLAK"
     gene            complement(2140..2541)
                     /locus_tag="PKV97_11865"
     CDS             complement(2140..2541)
                     /locus_tag="PKV97_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06620.1"
                     /translation="MSEPAFNFRHGKRGDARRQLAALGLALVLLAGLWPQPAEAQSPR
                     ARERRVERSLAAKDAIRHRQMKRAYVAGAVAQHRRERIRDRYEDRYYRDRYERYDRRD
                     DDDLSRVLIGVAVGAVATSIIMNSHQDGDAR"
     gene            complement(2541..2903)
                     /locus_tag="PKV97_11870"
     CDS             complement(2541..2903)
                     /locus_tag="PKV97_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06621.1"
                     /translation="MKIRPFVCSLALCLGLSAPNAGAEPAQGDEMERARCSGIIDLFV
                     AADPALEKDEQALAAAQDRAYVFLMWAHGYLSGRDGIDFARRPLNQDGITALVGEIYA
                     VCRNDEARLFLDAVKEIR"
     gene            complement(2960..3589)
                     /locus_tag="PKV97_11875"
     CDS             complement(2960..3589)
                     /locus_tag="PKV97_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HNB06622.1"
                     /translation="MKMLRLALPCALMLGLSACSTTPALRAPEDYPRLDVKADEPNTL
                     VFVKPGIELSRYTKVFVAPVGVQIGEEGARHDVGDAEARAVAAFAERVLKEKLSRTRQ
                     LVTAPAPDVLSVRFRIIGLEPTSGAQMVMMVPPFALINMVSPKGLFIGSVTVGGELYE
                     GLASTPSVAFVATRSRPGIDAASAFGRWSAAEKVLENAAERLATDLDRP"
     gene            complement(3586..3837)
                     /locus_tag="PKV97_11880"
     CDS             complement(3586..3837)
                     /locus_tag="PKV97_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNB06623.1"
                     /translation="MKLILSGLSPETDLANLTKRMSRFGPVRGVRPVREGDPQRPWFI
                     VDLDLPHGAAVAVARRIDGIYFNGRFLHASVMLPIGSDR"
     gene            3959..4426
                     /locus_tag="PKV97_11885"
     CDS             3959..4426
                     /locus_tag="PKV97_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06624.1"
                     /translation="MDDFDRRFEKTFAMVAFASNRHLVDHMRRIINLLEIDAESAMLW
                     GLVAHLSVAHAMHPGAQPADLLAPDGFLLGEARPVRLADLVQVSGLPKETVRRKLEKL
                     RERGKLGRTEDGRWVVLRSGVDETTYEFTRESVKRLLQTARVIEGILQHARLD"
     gene            complement(4493..4831)
                     /locus_tag="PKV97_11890"
     CDS             complement(4493..4831)
                     /locus_tag="PKV97_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06625.1"
                     /translation="MVIRDIFRFARVSTAVGLLCVVLLLACAQAIPEDAGKLMNGVRE
                     SLNVFAWVAMWRPAELWLYAHLPERHWRRLSLRLGQAKVGVERPAATITRASASGTPR
                     GPASETVAAT"
     gene            complement(4866..>5036)
                     /locus_tag="PKV97_11895"
     CDS             complement(4866..>5036)
                     /locus_tag="PKV97_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HNB06626.1"
                     /translation="VEDFVERSGYDALPLALVDGELAVAGRHPTRSELARWAGLAQPI
                     PAGGCCSGGHCG"
BASE COUNT          814 a         1749 c         1736 g          737 t
ORIGIN      
        1 cgagcgggac ggtgatggcg gcgtccgccg cgggcgcccc gccggcgtcc gccgccggtt
       61 ccaggctcac cgccatcccc ggcagcaggt tgacgccggc aggcaccgag cgcaccgcga
      121 gcaccaccgc ataggtctgc gtcagcgcgt cggcctcggc ggcgaaggac tgcagctcga
      181 gcctcagcgc ctgccccggc gcggcgcggt cgccctccac gctgcgctcg ccctcgagaa
      241 atgcgaccgc agcttgctgc ggccgcatgc ggcgcaccag acgctcgggc acattcacca
      301 cgacctcgag cgcgcgcagg tcctgcagct cggcgatcgg ctgcttggcc tgcacattgg
      361 tgaagggctc gatgcggcgg cgggtgatca cgccctcgaa aggcgccttg atcgcagtgt
      421 cggcgagatc gcgctcggcg gcggcgaggc tggtgcgcgc ggcttcgagg cgggcgcggc
      481 ggtcgtcgac ctcggcgcgg gcgaccgcct gctcgctttc ccacaggcgc tgatagcgcg
      541 cgagatcgtt ccgcgcacgc tcgaactccg cacgcgcggc gtccagacgc gagcggtaga
      601 tgcggtcgtc aaggcgcgcg accacctccc cagccttcac ccgccgcccc tcctccaggc
      661 tgaaggagtc gacgaagccg ggcacgtcga acgacaactc ggcgcgcctg gctgcacgca
      721 cccgcccggg caggcgcaag gcctcgtccg ccgtgcgccg tccgacggtg agaatcggcg
      781 ccggacgcgt cgcctccacc gccgcaacgg gcgcgggccg ctcgctgcag gcggccaacg
      841 acaggcaaag cagcgcggta gtcagcgcga aaactgattt ttgaatgcgc atgaatttct
      901 tctggtcgga tgcgggcggt cggggcgcgg gtcgcgtgcg cactgccggc gcggcgcagc
      961 caccagacgt gctcatcgcg caaggcctgt ttacacgacg cgtgcatgcg gggcacgcac
     1021 gacgcgggac cgatcgtcag ttgggtcggt caagtatccg cttgcgcagc agatcgtagt
     1081 cgccgcgatc gatcgcaccc tcgtcgagcg cgcgctgcag gtcggccagc tcctgcccct
     1141 tcgagatcgt cgtggtgccc tgcaccttca cataggtgtc cccgccgcaa ctcgccaaca
     1201 gcaggcaacc gaggacggcc agcaagggaa aagctttcgt gcgcatggtt cactccacgc
     1261 tgtcgatcag gcgttcgcgc tggcgctcgt actcgcggtc cgagagggcg cccgcctcgc
     1321 gcgcacgctt gaggtcgatg agctgctggc cgatggagac gtccaccgta ggccgtacga
     1381 cttcgtggcc accgcaaccc gtcagcgaca agccgatcag cccggcgaag agggcaagcc
     1441 tggtattcat tgaaaggatc ccatgagcgt ggcgacaggc acgaccgaaa ccggccatgc
     1501 gggacgggcg aaaccgattt gcgcagctta atccggcccg catgccccat gtccgcgttc
     1561 gtcccaaaat gggccaggcc gacgcaacag gcgcccgcct gcaggcactc ggctgcgaac
     1621 atcgcttcgg ctggccggcc actgcctact tcgccagggg gatctgctgg agcacacgac
     1681 tgccgatcga cgcatccatc gccagttcaa gctgatacag ccgcagcgtc tggatgggcg
     1741 tgatcacctc acccgccttc cggatcatgt ggtggcgggc gtccacccga gcctgctcca
     1801 gcgcgagcga gcgtgccagc gaggtgcgcg ccgtggggtc gtcgatccag cccgcgtcga
     1861 gctgcctggc gtactcggca agcgtgtcga tctgacggtc acgcaaggcg acgatctcgg
     1921 ccaggtaggc gcgatagatc ggccagaagc gcttcgcacc ctgctcgtcg agcttcatgc
     1981 tggcgctgac ctcgatcacg cggcgcaggt ccacatcctg gaactgctcg cggatcagac
     2041 cgagggtcgc gtcctcctcg ggcgttccgg catgcgcacc caaggccagc ccggcgcaaa
     2101 ggacgacgcc ggcaaggtaa cgggttgcag atcggatcgt catcgtgcgt ccccatcctg
     2161 gtgactgttc atgatgatgc tcgtggcgac cgcgccgact gcgaccccga tcaacacgcg
     2221 gctgagatcg tcatcgtcgc gacggtcgta gcgctcgtaa cggtcgcggt aatagcggtc
     2281 ctcgtaacgg tcccggatcc gctcgcgccg atgctgcgcg accgcgccgg cgacataggc
     2341 ccgtttcatc tgccgatgcc ggatcgcatc cttggccgcc agcgaacgct ccacccgccg
     2401 ctcgcgtgcg cgtggcgatt gcgcctcggc cggttgcggc cacaggcccg cgagcaggac
     2461 cagggcgagg cccagcgcgg cgagttgccg gcgcgcgtcg ccgcgcttgc cgtgacgaaa
     2521 gttgaaggcc ggttcgctca tcagcggatc tccttcaccg catcgaggaa cagacgcgcc
     2581 tcgtcgttcc tgcacaccgc gtagatctcg cccaccagcg cggtgatgcc gtcctggttc
     2641 agcggccggc gtgcgaagtc gatgccgtcg cggccgctca ggtagccgtg ggcccacatc
     2701 aggaagacgt aggcacgatc ctgggcggcg gccagcgcct gttcgtcctt ctcgagcgct
     2761 gggtcggccg ctacgaacag atcgatgatg ccactgcagc gggcacgctc catctcgtcg
     2821 ccctgtgcgg gttcggcccc cgcgttcggc gcggaaagac ccaggcacag cgccaggctg
     2881 cagacaaacg gacgaatttt catgtcaggt ttccttcgag ggagacggca atcaggacgg
     2941 cgcatgcgcg gccgagggct cagggccgat cgaggtccgt cgccaggcgc tcggcggcgt
     3001 tctccagcac cttctcggca gccgaccagc gcccgaacgc cgaggcggcg tcgatgcccg
     3061 gccggctgcg ggtggcgacg aaggcgacgc tgggcgtgct ggccagcccc tcgtagagtt
     3121 cgccgccgac ggtgaccgag ccgatgaaga gccccttcgg gctcaccatg ttgatgaggg
     3181 cgaagggcgg caccatcatc accatctggg caccgctcgt cggctcgagc ccgatgatgc
     3241 ggaagcgcac gctcagcacg tccggcgcgg gcgcggtgac gagttgccgg gttcgcgaca
     3301 gtttctcctt cagcacccgc tcggcgaacg ccgccaccgc acgcgcctcg gcgtcgccga
     3361 catcgtgacg cgccccctct tcaccgatct gcacgcccac cggggcgacg aagaccttcg
     3421 tgtagcggct cagctcgatg cccggcttga cgaacacgag ggtgttgggc tcgtcggcct
     3481 tgacgtcgag ccgggggtaa tcctccggtg cgcgcagcgc cggagtggtg gaacaggcgc
     3541 tcaggcccag catgagcgca cacggcaggg caaggcgtag catcttcatc tatcggatcc
     3601 gatcgggagc atcacgctcg cgtgcaggaa gcggccgttg aaatagatgc cgtcgatgcg
     3661 gcgcgcgacc gcaaccgcgg cgccgtgcgg caggtcgagg tcgacgatga accatgggcg
     3721 ctgcggatcg ccctcgcgga ccggtcggac gccacgcacc gggccgaaac ggctcatgcg
     3781 cttggtgagg ttggcgagat ccgtttccgg actcaggccc gacaggatca gcttcaagtg
     3841 cggcctccgg cagctggaac tggattgggt cgaaaaggcg ttgcaggata aatcgggcgc
     3901 agggaaaatc ggcggcaaac ggcccatttc gggccgccgc tcgtctggag acatcgagat
     3961 ggacgatttc gaccggcgct tcgagaagac cttcgcgatg gtggcctttg cgagtaaccg
     4021 ccacctggtc gatcacatgc gacgcatcat caacttgctc gagatcgacg cggagagcgc
     4081 catgttgtgg ggcctggtcg cgcacctcag cgtcgcgcat gccatgcatc ccggcgcgca
     4141 gccggcggac ctgctcgcgc ccgacggctt cctgctcggc gaggcacgcc cggttcgcct
     4201 cgccgatctg gtgcaggtgt cagggctgcc gaaggagacc gtgcggcgca agctggaaaa
     4261 gctgcgcgag cgcggcaagc tcggacgcac cgaggacggg cgctgggtgg tgctgcggag
     4321 cggcgtcgac gagaccacct acgagttcac ccgcgagtcg gtcaagcgcc tgctgcagac
     4381 cgcgcgcgtg atcgagggca tcctgcagca cgcgcgcctg gactgaaccg gcgccgactg
     4441 ccggcggcag gggcggacag ggtggctcgg tccgggggaa cggggaatcc gttcaggttg
     4501 cggcgacggt ctcggaggca gggccgcgag gcgttcccga tgcgcttgcg cgagtaatcg
     4561 tcgcggccgg tcgctccacg cccaccttcg cctggccgag gcgcagggag aggcggcgcc
     4621 agtggcgttc gggcagatgg gcgtacagcc acagctcggc gggtcgccac atcgccaccc
     4681 aggcgaagac gttcaggctc tcgcgcaccc cgttcatcag cttccccgcg tcctcgggga
     4741 tcgcctgtgc gcaggcgagc agcagcacca cgcaaagcag accgaccgcg gtcgacaccc
     4801 gcgcgaagcg gaagatgtcg cggatcacca cgcccgcagg gtcgaacgaa ggagcagacc
     4861 ccgcctcagc cgcaatggcc gcccgagcag cacccccccg ccggaatcgg ctgcgccagc
     4921 ccggcccagc gcgcgagctc gctgcgcgtg ggatggcggc cggccacggc cagctcgccg
     4981 tcgaccaggg ccagcggcag ggcgtcatat ccggagcgct cgacgaagtc ctcgac
//
LOCUS       DAOQNN010000238         5022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10112_length_5022_cov_9.570968, whole genome shotgun sequence.
ACCESSION   DAOQNN010000238 DAOQNN010000000
VERSION     DAOQNN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10112_length_5022_cov_9.570968"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PKV97_11900"
     CDS             <1..683
                     /locus_tag="PKV97_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HNB06627.1"
                     /translation="FLDHLVPDDRLRLREMALAFLGCKEFHGAQGLELDDDMLLAIAL
                     QACLPVLHIGLQAYEGWVGVIVYPGDFLIPRRIVDEAGVLHEYEDEVLGEAWAGGPVL
                     LSWFPGNDAPAGVNVVIHEFAHKLDMENGDVDGLPRLRPGMSRRDWAKAFGEAYEAFC
                     AEVDRGVDTTIDPYAAEHPGEFFAVVSEVFFEDPLVLHRRFPAVYDQLVHFYGMDPAA
                     RAAAAAGG"
     gene            685..1149
                     /locus_tag="PKV97_11905"
     CDS             685..1149
                     /locus_tag="PKV97_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNB06628.1"
                     /translation="MSAPKRLLIVHHTQSGNTGRLAAAVLEGALRVSETETRLLRAFD
                     AGTADLLACDGLLLGTPENFGYMSGALKDFFDRTYYPCEGKLVGLPYAVFVSAGNDGT
                     GAVREIGRIANGYGWKMVAEALIVRREITPADLERARELGEAMAAGLAMGVF"
     gene            1356..1796
                     /locus_tag="PKV97_11910"
     CDS             1356..1796
                     /locus_tag="PKV97_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HNB06629.1"
                     /translation="MAITWILVANASLAKLYANLGPNKGLTLVKELIHPESRQKNGDL
                     VSDRSGANGSNGTGGGSMQPQTLPKQHEAKVFAQEIAQTLYHGRTSNAFKRAILVAPP
                     AFMGLLNAVIDGPTAQMVTDRFEKDYTKSTQDELGERLGSTIFL"
     gene            complement(2028..2588)
                     /gene="thpR"
                     /locus_tag="PKV97_11915"
     CDS             complement(2028..2588)
                     /gene="thpR"
                     /locus_tag="PKV97_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNB06630.1"
                     /translation="MAEAAADAGRAPNLRLFFALWPDAATRAGLHARARALHSECGGR
                     AMRRDTIHLTLAFLGETPVRQVERLRAVAAGVQGDGFALDLDRVGSWHRNRILWTGPS
                     MVPPALAALAQDLEGRLRADGCALEARAFSPHVTLVRNAHAAPREAQLTPLRWTVGSF
                     VLVASERSAAGAHYRTIGRWPLKRRG"
     gene            complement(2581..3075)
                     /gene="ispF"
                     /locus_tag="PKV97_11920"
     CDS             complement(2581..3075)
                     /gene="ispF"
                     /locus_tag="PKV97_11920"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNB06631.1"
                     /translation="MSIPFRIGQGFDVHALVPGRPLIVGGVDIPFGHGLLGHSDADVL
                     LHAVTDALLGAAGLGDIGRLFPDTDPAHAGADSRVLLREAFARVRAAGWEVVNLDATL
                     ICKAPRILPHAPAMVANLAADLEMDATAINIKGKTTEKLGFTGRGEGIAAQVVALIAR
                     AEHG"
     gene            complement(3072..3782)
                     /gene="ispD"
                     /locus_tag="PKV97_11925"
     CDS             complement(3072..3782)
                     /gene="ispD"
                     /locus_tag="PKV97_11925"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNB06632.1"
                     /translation="MQISHPRHFAIVPAAGSGSRMAAARPKQYLSLLGRPLIHHALSV
                     LCAAPAIDAVFVVLSVDDTEWATHDWSALGPKLRPLFCGGATRADSVLGGLRAIAGEA
                     AAGDWVLVHDAARPCLAPWHVDKLVRELAHDEVGGLLAVPVADTLKRADEHRHVRETV
                     PRDSLWQAQTPQMFRYAMLRRALEGAREVTDEASAIEAAGLRPRLVQGDATNLKVTYP
                     LDLHLAEWILENREGHNA"
     gene            3912..4709
                     /gene="amrB"
                     /locus_tag="PKV97_11930"
     CDS             3912..4709
                     /gene="amrB"
                     /locus_tag="PKV97_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNB06633.1"
                     /translation="MALASIRPAAVAGQFYPRDERVLRTQLAEMLSTAVAFESAPAPK
                     AIIVPHAGYMYSGPVAASAYALLAPLRERIRRVVMLGPTHRVAVRGFSLPAAQRFATP
                     LGEVPVARGDWEALQARDDVLVDDRPHALEHCLEVQLPFLQVCLERFEIVPVLVGDAS
                     PEATASLIERLWGGPETLILISSDLSHYLSYREAQWSDRAAVDQVLELRPGLDHEQAC
                     GATPINGLLLAARKHDLQAHLLDLRNSGDTAGDRSRVVGYTSIAFPD"
     gene            4784..>5022
                     /locus_tag="PKV97_11935"
     CDS             4784..>5022
                     /locus_tag="PKV97_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043745823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNB06634.1"
                     /translation="MPMNRIQFQAGLSMPEFLQRYGTEPSCEAALEKARWPAGFRCPR
                     CDGVTYSRVRGRTYALFQCQACRHQTSLIAGTVMQG"
BASE COUNT          780 a         1741 c         1719 g          782 t
ORIGIN      
        1 ccttcctcga ccacctcgtt cccgacgatc ggttgcgtct gcgcgagatg gcgctcgcct
       61 tcctcgggtg caaggagttc catggtgcgc aggggctgga actcgacgac gacatgctgc
      121 tggcgatcgc gctgcaggcc tgcctgccgg tacttcacat cggcctgcag gcttacgagg
      181 gctgggtggg cgtgatcgtg tatccgggcg acttcctgat cccgcgccgc atcgtcgacg
      241 aggccggcgt gctgcacgaa tacgaggacg aggtgctggg cgaggcctgg gccggcgggc
      301 cggtgctgct gtcctggttt cccgggaacg acgctccggc gggcgtgaac gtggtcattc
      361 acgagttcgc ccacaagctc gacatggaga acggcgacgt ggacggcctg ccccgcctgc
      421 gccccggcat gtctcgccgg gactgggcca aggccttcgg cgaggcctac gaggcgttct
      481 gcgccgaggt cgaccgcggc gtcgacacca cgatcgaccc ttatgccgcc gagcacccgg
      541 gtgagttctt cgcggtggtc tccgaggtct tcttcgagga tccgctcgtg ctgcacaggc
      601 gctttcccgc ggtctacgat caactcgtgc acttctacgg aatggacccc gccgcccgcg
      661 cggcggccgc tgccggaggc tgacatgagc gcgcccaaac gcctgctgat cgtccatcac
      721 acccagagcg gcaacacggg ccggctcgcc gccgcggtgc tcgagggggc gctgcgcgtg
      781 agcgagaccg agacgcggtt gctgcgcgcc ttcgacgccg gcaccgccga cctgctcgcg
      841 tgcgacggcc tgctgctcgg tacgccggaa aacttcggct acatgtcggg cgcgctcaag
      901 gacttcttcg atcgcaccta ctacccctgc gagggcaaac tcgtcggcct gccctatgcg
      961 gtcttcgtca gcgccggcaa cgacggcacc ggcgcagtgc gcgagatcgg ccgcatcgcc
     1021 aacggctacg gctggaagat ggtcgccgag gccctgatcg tgcggcgcga gatcaccccc
     1081 gccgacctgg agcgcgcgcg cgagctcggc gaggcgatgg cggccgggtt ggcgatgggg
     1141 gtgttctgag cgtgcgaggg gcgaggcttc gggcgtgcga tgccgcgggg acgcggtcgc
     1201 ggcgagcacg tcgggtaagt ttacgtttgc gtcaatcggg atcggttttc acgaagcggt
     1261 aacgatggtc gtggcggagt cggttcgcgt gacctaagct tcgatcgtgc ccactgcgca
     1321 gggcacccgg tctttaatcg aacggaggat tgcttatggc catcacctgg attctggttg
     1381 ccaacgccag cctggcgaag ctgtacgcca acctgggccc gaacaagggg ctcacgctgg
     1441 tcaaggaact gatccacccg gaaagcagac aaaagaatgg cgatttggtc tcggaccgct
     1501 cgggcgcgaa tggctcgaac ggcaccggtg gcggctcgat gcagccgcag accctgccca
     1561 agcagcacga ggccaaagtg ttcgcacagg agattgccca gacgctctac catggacgca
     1621 cctcgaatgc attcaagcgt gcgatcctgg tcgctccacc cgccttcatg ggcctgctca
     1681 acgcggtcat cgacggcccc accgcgcaga tggtcacaga ccgcttcgaa aaggattaca
     1741 ccaagtcgac gcaggacgag ctgggggaac gcctcggctc gacgatcttt ctctgaggga
     1801 ggacgttcac gaaggctcca gatccgatca gccaatggcc gagggcacct gcccgagcca
     1861 aggaggatgt gcttgaccga acccccgagg atgcagcttt cctgacggat gcagtacaca
     1921 cgatgaagac atcgtctcca ctacggagac cccgggcacg cgagacgtga cccgaataag
     1981 gacggggcgc attgcgcccc gttcgctttc ggctccgccc gatcgaatca cccacgccgc
     2041 ttcaacggcc agcgcccgat cgtgcggtag tgcgcaccgg cggcgctgcg ctcggaggcg
     2101 accaggacga agctgccgac cgtccatcgc agaggcgtga gctgcgcctc gcggggcgcc
     2161 gcgtgggcgt tgcgcacgag ggtgacatgc ggggagaagg cacgcgcctc gagtgcgcag
     2221 ccgtccgcgc gcagccgtcc ttccagatcc tgcgcgagcg cggccagcgc cggcggcacc
     2281 atcgacggac cggtccacag gatccggttg cgatgccagc tgccgacgcg atcgaggtcg
     2341 agcgcgaagc cgtccccctg gacgcccgcg gccaccgcgc gcaatcgctc cacctgccgc
     2401 acaggcgttt cgccgaggaa ggccagggtc aggtggatgg tgtcacgacg catcgcgcga
     2461 ccgccgcact cggaatgcag cgcccgtgca cgcgcatgca gcccggcacg ggtggcggca
     2521 tccggccaca gcgcgaagaa gagccgcaga ttcggcgccc tccccgcgtc cgctgcagct
     2581 tcagccatgc tccgcgcgcg cgatcagcgc gaccacctgc gcggcgatgc cttcgccacg
     2641 tccggtgaag ccgagcttct cggtggtctt gcccttgatg ttgatcgccg tcgcgtccat
     2701 ctccaggtcg gcggccaggt tggccaccat cgccggggcg tgcggcagga tgcgcggcgc
     2761 cttgcagatc agcgtggcat cgaggttcac gacctcccag cccgctgcgc gcacgcgcgc
     2821 gaaggcctcg cgcagcagca cgcggctgtc ggcgccggcg tgggccgggt cggtatccgg
     2881 gaagagccgg ccgatatcgc ccaggccggc ggcgccgagc agcgcgtccg tcaccgcatg
     2941 caacagcacg tcggcatccg agtggccgag cagtccatgc ccgaagggaa tgtcgacgcc
     3001 gccgacgatc agcggccggc cggggaccag ggcgtgcacg tcgaagccct ggccgatgcg
     3061 aaaggggatg ctcatgcgtt gtggccctca cggttctcga ggatccactc ggccaggtgc
     3121 aggtcgagcg gataggtgac cttcaggttg gtggcgtcgc cctgcaccag gcgcggacgc
     3181 aggccggcgg cctcgatcgc gctcgcctcg tcggtgacct cgcgcgcgcc ttcgagcgcg
     3241 cgtcgcagca tggcgtagcg gaacatctgc ggcgtctgcg cctgccacag gctgtcgcgc
     3301 ggcacggtct cgcgcacatg gcggtgctcg tcggcacgct tgagggtgtc ggccaccggg
     3361 actgccagca agccgccgac ctcgtcgtgg gcgagctcgc gtaccagctt gtcgacatgc
     3421 cagggcgcca ggcaggggcg cgcggcgtcg tgcaccagca cccagtcccc tgccgccgcc
     3481 tcgccggcga ttgcgcgcag gccgccgagc acgctgtcgg cgcgcgtggc gccaccgcag
     3541 aagagcgggc gcagcttggg gccgagcgcg ctccagtcgt gcgttgccca ctccgtgtcg
     3601 tccaccgaga gcacgacgaa caccgcgtcg atcgcaggcg cggcgcacag caccgaaagc
     3661 gcatgatgga tcagggggcg accgagcagc gacagatact gcttgggacg cgcggcggcc
     3721 atgcgcgagc cgctgcctgc ggcgggcacg atggcgaagt ggcgtgggtg gctgatctgc
     3781 atggcgcgca ttctagccgc gccgcccgcc gcgcgggctt gtgcaagcac gggtttcgat
     3841 gcgggacgat cccggccggg ctgcctataa tggaatctgt tccaacgatg acatccccgc
     3901 aggattcctc catggcactc gcttcgatcc gcccggcggc cgtggccggg cagttttatc
     3961 ctcgcgacga gcgcgtgctg cgcacccagc tcgccgagat gctctccacc gcggtcgcct
     4021 tcgagtcggc gcccgcgccc aaggcgatca tcgtgccgca cgcgggctac atgtactccg
     4081 ggccggtggc ggcgagcgcc tatgcgctgc tcgcaccgct gcgcgagcgc atccgccgcg
     4141 tcgtcatgct cggccccacg catcgcgtgg cggtgcgcgg cttctcgctg ccggcagcgc
     4201 agcgctttgc caccccgctc ggcgaggtcc cggtcgcacg cggcgactgg gaggccctgc
     4261 aggcgcgcga cgatgtcctg gtcgacgatc gcccgcacgc gctcgagcac tgcctcgagg
     4321 tgcagttgcc cttcctgcag gtctgtctcg agcgcttcga gatcgtcccg gtgctggtcg
     4381 gcgacgcctc gcccgaagcc accgccagcc tgatcgagcg cctgtggggc ggccccgaga
     4441 ccctgatcct gatcagctcg gacctctcgc attacctgag ctaccgcgag gcgcaatggt
     4501 ccgaccgcgc cgcggtggac caggtgctcg aactgcgccc cggtctcgac cacgagcagg
     4561 cctgcggcgc caccccgatc aacgggctgc tgctcgccgc ccgcaagcac gacctgcagg
     4621 cccacctgct ggacctgcgc aactccggcg acaccgcggg cgaccgctcg cgcgtcgtcg
     4681 gctacaccag catcgccttt cctgattagc aacgatgctc agccaggcac cccaacgggt
     4741 ctatactgta cggaaataca gtttttctgc cgggaggtgc gccatgccga tgaaccgaat
     4801 ccagtttcaa gccggactgt ccatgcccga gtttctccag cgctacggaa ccgagccgtc
     4861 ctgcgaggcg gctttggaga aggcacgctg gcctgcgggg tttcggtgtc cgcgctgtga
     4921 tggcgttact tacagccggg tacgcgggcg aacctacgcg ctgttccagt gccaggcgtg
     4981 tcgacatcaa acgtcgctga tcgccggcac cgtgatgcag gg
//
LOCUS       DAOQNN010000239         5015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10135_length_5015_cov_11.173387, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000239 DAOQNN010000000
VERSION     DAOQNN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10135_length_5015_cov_11.173387"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PKV97_11940"
     CDS             <1..770
                     /locus_tag="PKV97_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNB06635.1"
                     /translation="GLYLQDRHGQPLQAGAAPDEAMQAMFARAQNEGRDLYEHEARAL
                     LRTHGVAVAEEIVAHTADELAVAIERFRDQPLAMKVVSKDILHKSDAGGVKLNLVGEA
                     ALREAWAQILAGCRAYDADADIRGVLLTPMARKGVEVIVGVVRDPIFGPVLMFGLGGI
                     FVEILEDVAFRAIPLSRRDAETMVDQLGARKVLAGVRGEAAVDKVALVELLLRVSSIV
                     SAYPQLSELDLNPVIAYPDGYAVVDARIITNRTVASS"
     gene            987..2855
                     /locus_tag="PKV97_11945"
     CDS             987..2855
                     /locus_tag="PKV97_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB06636.1"
                     /translation="MENPEMARKRRSTIIWVIALAFVGLVFDGYDLVVYGAVLPGFMG
                     PDSAKWIGDHALTPKDAGMLGSYAMIGMMLGALVAGTLGDLIGRRKVMIGAIAWFSIG
                     MAVSAMTETLAAFGVWRFITGLGVGALAGTTGAMAAEFAPPGKKNLATAFTYAGIPLG
                     SLLSALLAIFLLPIIGWRGMFLIGALPLVTLLPLAIWKLPESVAWLASRGRLDEARKV
                     SERTGVPIPEAEIKPQQSAAANASDARAGWAGLFTVYLVPAIVIGFVSAFCLLLVYSL
                     NTWLPKIMLPIMGQNGSLALLLVLNAGAMVGTLYGSTLADKHSPQRIVALGFLVGALA
                     MGAIGMIASTIDVPMVAKAGEIQQLADVSGIVVALLLLTIAITGLGTSGTQTLIYGLA
                     ANYYRTNVRGAGVAWTAAFGRIGGIFGPIFGAFLAVTFAGELHSIFYVLGGISLVGLA
                     LTLIIPKSKAAAVAQAVAPTTAAAAAVPAGKSRLYGTIMAVVDHTGNRLTRERVSEFV
                     SLTGSKVHLVYLAPEHVLSAEVDQTDKAIDPAHVANLQEYVNEVNAQGVPAEGQILTS
                     TLFGRGRAVVDLAEQLRCDLIILNTEEGGQRAKAELAQEVAGQNPKMAVLIARSTQ"
     gene            complement(3029..3211)
                     /locus_tag="PKV97_11950"
     CDS             complement(3029..3211)
                     /locus_tag="PKV97_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06637.1"
                     /translation="MHIYFSHDGFLALALGLWELLRALRPKIAQTLTITARDPHPAPS
                     RSEESDVRAPQWRRHL"
     gene            3191..>3606
                     /locus_tag="PKV97_11955"
     CDS             3191..>3606
                     /locus_tag="PKV97_11955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB06638.1"
                     /translation="MRKVDVHQTIDEAKFNKFHLNVLFWCALVIIFDGYDLVIYGVVL
                     PVLMKEWDLTPLQAGSLGSAALFGMMFGALIFGPLSDRIGRKKVIMITVIIFSGVTFL
                     NGFAETPTQFAAMRFIAGLGIGGVMPNVVALMTEYMP"
     assembly_gap    3607..3706
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3885..4916
                     /locus_tag="PKV97_11960"
     CDS             3885..4916
                     /locus_tag="PKV97_11960"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNB06639.1"
                     /translation="MELEAYVRALPKAELHLHIEGTLEPEMMFALARRNGVALPWASV
                     EAVRAAYAFTDLQSFLDLYYAGAAVLVREQDFFELAFAYFERAHADGVVHAELFFDPQ
                     THTARGVALETVLDGLERACVEARARWGIGSRLILCFLRHLSEEEGFATLQQALPHLS
                     RIDGVGLDSSERGHPPAKFARLFARCRELGLHVVAHAGEEGPPAYIVDALDLLKAERI
                     DHGVRCTEDPALVGRLVREQVPLTVCPLSNVKLCVFPDLARHNLGQLFAAGLKVTINS
                     DDPAYFGGYVAKNYVDTARALGLGRAELRRIARNSLEASFVSAAERAPWLARLDALGE
                     DCEGEGGAA"
BASE COUNT          737 a         1675 c         1664 g          839 t
ORIGIN      
        1 gcgggctcta cctgcaggac cgccacggcc agcccctgca ggccggcgcg gcgcccgacg
       61 aggcgatgca ggcgatgttc gcgcgcgccc agaacgaggg ccgcgacctc tacgagcacg
      121 aggccagggc gctgctgcgc acgcacggtg tcgcggtggc ggaggaaatc gtcgcccaca
      181 cggcggacga gcttgcggtg gcgatcgagc gcttccgcga ccagccgctg gcgatgaagg
      241 tggtgtcgaa ggacatcctg cacaagtccg acgccggcgg ggtgaagctg aacctggtcg
      301 gcgaggcggc gctgcgcgag gcctgggcac agattctggc gggctgtcgg gcatacgatg
      361 ccgacgccga cattcgcggt gtgctgctga caccgatggc gaggaagggg gtggaggtca
      421 tcgtcggcgt ggtgcgcgac ccgattttcg gcccggtgct gatgttcggc ctgggcggca
      481 tcttcgtcga gatcctcgag gacgtcgcct tccgcgcgat tcccctgtcc cggcgcgacg
      541 ccgagaccat ggtcgatcag ctcggcgcgc gcaaggtcct cgccggcgtg cgtggcgagg
      601 cggcggtgga taaggtcgcg ctggtcgagc tgctgctgcg ggtgtcgagc atcgtcagtg
      661 cctacccgca gctctccgag ctcgacctca acccggtgat cgcctacccg gacggctatg
      721 cggtggtcga cgcgcgcatc atcaccaacc gcacggtggc cagcagctga gccggtcgag
      781 cggattttcg cgccttggat cggggtgtgc acgaagcgga tatcgatcgt gcacaagctg
      841 gatattgatc ggtgtgatcg cgccataacc tgacggcctt gcaatcaagg aagcttgacg
      901 cacgtcaagt tcgtcactgc tcgcccgtcc gatctcccct gcagcaattc ccccaaagag
      961 gcacgatttt gaaaacgaac gttccgatgg aaaacccgga gatggcgcgc aagcggcgca
     1021 gcaccatcat ctgggtgatc gccctggcct tcgtcggcct ggtcttcgat ggctacgacc
     1081 tcgtcgtcta tggcgcagtc ctgcccggct tcatggggcc ggactccgcc aagtggatcg
     1141 gcgaccacgc actcacgccg aaagacgccg gcatgctcgg ctcgtacgcg atgatcggca
     1201 tgatgctcgg cgccctcgtc gccggcaccc tgggcgatct gatcggccgc cgcaaggtca
     1261 tgatcggcgc catcgcctgg ttctcgatcg gcatggcggt gtcggcgatg accgagacgc
     1321 tcgccgcctt cggcgtctgg cgcttcatca ccggcctggg cgtgggcgcg ctggcgggca
     1381 ccacgggcgc gatggcggcc gagttcgccc cgccggggaa gaagaacctc gccaccgcct
     1441 tcacctatgc cggcatcccg ctgggttcgc tgctgtctgc gctgctggcg atcttcctgc
     1501 tgccgatcat cggctggcgc ggcatgttcc tgatcggcgc gctgcccctg gtcaccctgc
     1561 tgccgctggc gatctggaag cttcccgagt cggtggcctg gctggcctcg cgcggccgcc
     1621 tggacgaggc ccgcaaggtc tccgagcgca ccggcgtgcc catccccgag gcggagatca
     1681 agccgcagca gagcgcggcc gccaatgcct cggacgcgcg tgcgggctgg gcgggtctgt
     1741 tcaccgtcta tctcgtcccg gccatcgtca tcggcttcgt ctcggcgttc tgcctgctgc
     1801 tggtctattc gctgaacacc tggctgccga agatcatgct gccgatcatg ggccagaacg
     1861 gatcgctcgc attgctgctg gtcctcaacg ccggcgccat ggtcggtacc ctgtacggct
     1921 ccaccctggc cgacaagcac agcccgcagc gcatcgtggc gctgggcttc ctggtcggcg
     1981 cgctcgccat gggcgccatc ggcatgatcg cctccacgat cgacgtcccg atggtcgcca
     2041 aagcaggcga gatccagcaa ctcgccgacg tcagcggcat cgtcgtggcc ctgctgctgc
     2101 ttacgatcgc gatcaccggc ctggggacct cggggacgca gacgctgatc tacggtctgg
     2161 ccgccaacta ctaccgcacc aacgtccgtg gcgccggtgt cgcctggacg gcggccttcg
     2221 gtcgtatcgg cggcatcttc gggccgatct tcggcgcctt cctggcggtc acgttcgccg
     2281 gcgagctgca cagcatcttc tacgtgctcg gcggcatcag cctcgtcggc ctggccctca
     2341 ccctgatcat tccgaagagc aaggcggcgg cggttgcaca ggcggtggcg ccgaccacgg
     2401 cggctgcggc ggccgtgccc gccggcaagt cccgtctcta cggcaccatc atggccgtgg
     2461 tcgatcacac cggaaacagg ctgacgcgcg agcgcgtctc cgagttcgtc tccctgacgg
     2521 gctcgaaggt gcacctggtg tatctcgcgc cggagcacgt cctgtccgcc gaggtcgatc
     2581 agaccgacaa ggccatcgac ccggcgcacg tggcgaacct gcaggagtat gtgaacgagg
     2641 tgaacgcgca gggtgtcccg gcggagggcc agatcctgac ctccacgctg ttcggtcgcg
     2701 gcagggcggt ggtcgacctg gccgagcagc tgcgctgcga cctgatcatc ctcaacaccg
     2761 aggagggcgg ccagcgtgcc aaggccgagc tcgcgcagga ggtggccgga cagaacccga
     2821 agatggcggt gctgatcgcg cgctcgacgc agtaagacgc tgccgcacca cgaagcccgc
     2881 cccgaccgct acggtcgggg cgggcttttt tgtgcgcggc gcaaccgcgc ctcgcacccg
     2941 gggtgggcaa caggtgtatg agatattgtg cagtcgaaat gtgttcatgc gcaaaaatgt
     3001 gcgatatagt gcatttttgg cgcctgggtc acaggtgcct gcgccattgc ggcgcacgaa
     3061 catcgctctc ttcgctccgt gatggggcgg gatgcgggtc gcgcgccgtg atcgtgagcg
     3121 tctgcgcgat cttcggtcgc aatgcccgca gcaactccca caaccccaac gcaagcgcca
     3181 ggaatccatc gtgagaaaag tagatgtgca tcaaacaatc gacgaggcga agttcaacaa
     3241 atttcacctg aacgtgctgt tctggtgtgc gctcgtcatc atcttcgacg gctacgacct
     3301 ggtgatctac ggggtcgtgc tgccggtgct gatgaaggag tgggacctca cgccgctgca
     3361 ggcgggctcg ctcggcagcg ccgcgctgtt cggcatgatg ttcggcgcgc tgatcttcgg
     3421 gccgctgtcc gaccgcatcg gccgcaagaa ggtgatcatg atcacggtga tcatcttcag
     3481 cggcgtgacc ttcctcaatg gcttcgccga gacgcccacc cagttcgccg cgatgcgctt
     3541 catcgccggc ctcggcatcg gcggcgtgat gcccaacgtg gtcgcgctga tgaccgagta
     3601 catgccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgag accggcaagc
     3721 tcgaggtgga ttgaggcgat tccgggccat gggcaaccgt cggcgcaccg gatcgatgcg
     3781 ggaacggccg gtgccgttcc cgcattcttg cgttcgggtg aatgtggcgc gggaacagcg
     3841 gcccgattgg cgctagcatt gaagtcccgc ccaggagacc cgccatggag ctcgaagcct
     3901 acgtccgcgc cttgcccaag gccgaactgc acctgcatat cgagggcacg ctcgagcccg
     3961 aaatgatgtt cgcgctcgcg cgccgcaacg gcgtggcgct gccctgggcg agcgtcgagg
     4021 cggtgcgcgc cgcctacgcc ttcaccgatc tgcaaagctt tctcgacctc tactacgccg
     4081 gcgcggcggt gctggtccgc gagcaggact tcttcgagct cgcgttcgcc tacttcgagc
     4141 gcgcacacgc cgacggcgtg gtccatgccg agctcttctt cgacccgcag acgcataccg
     4201 cgcgcggggt ggcgctcgag accgtgctcg acggcctcga gcgcgcctgc gtcgaggcgc
     4261 gcgcgcgctg gggcatcggc tcgcgcctga tcctgtgctt cctgcgccat ctcagcgagg
     4321 aggagggctt cgccacgctg cagcaggctc tgccgcacct gtcgcgcatc gacggcgtcg
     4381 gcctcgactc gtccgagcgc gggcatccgc cggccaagtt cgcccgcctg ttcgcgcgct
     4441 gccgcgagct cggcctgcat gtggtcgccc atgccggcga ggaggggccg ccggcctaca
     4501 tcgtcgatgc gctcgacctg ctgaaggccg agcgcatcga ccacggcgtg cgctgcaccg
     4561 aggatccggc gctggtcgga cgcctcgtgc gcgagcaggt accgctcacc gtgtgcccgc
     4621 tctccaacgt caagctgtgc gtgttccccg acctcgcccg gcacaacctc gggcagctgt
     4681 tcgccgcggg gctcaaggtg acgatcaatt ccgacgaccc ggcctatttc ggcggctacg
     4741 tggcgaagaa ttatgtcgac accgcgcgcg cgctcggcct cggccgtgcc gagctgcgtc
     4801 gcatcgcgcg caacagcctg gaggcgagct ttgtgtccgc cgccgaacgc gcgccctggc
     4861 tcgcccggct cgacgcgctc ggggaggact gcgagggaga gggcggcgcg gcatgacgcc
     4921 accgactgcc gcagagcgcc gggcgctgcg tgaccgcctc accgcggtgc cgctgttcgc
     4981 cggcctgccc gccgtgcagc tggccgagct cgccg
//
LOCUS       DAOQNN010000240         5006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10159_length_5006_cov_9.124217, whole genome shotgun sequence.
ACCESSION   DAOQNN010000240 DAOQNN010000000
VERSION     DAOQNN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10159_length_5006_cov_9.124217"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PKV97_11965"
     CDS             complement(<1..696)
                     /locus_tag="PKV97_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNB06640.1"
                     /translation="MPAAAPVLDAAQLAEAFALFSRASEELSTAYAALQAQVGQLSER
                     LSVLMGALPAGVLVVDRAGTVVQANRAAESLFGGGLAGVAWAELAARLEDSGTPGEFV
                     LRRDPAAVDGDPRRLALSESELDSGAERILLVHDVTDTHRMRLTAERNERLAAMGEMV
                     AGLAHQLRTPLAAALLYTGNLRQPELDPAQRAMVADRALERLRYLERLIRDMLLFARG
                     DSAGRQRFAVCELL"
     gene            900..2618
                     /gene="fliF"
                     /locus_tag="PKV97_11970"
     CDS             900..2618
                     /gene="fliF"
                     /locus_tag="PKV97_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNB06641.1"
                     /translation="MAAAENTADLPPGAAPADAQATTRMQQALQNLAALSTRQKIAGA
                     VAIALAIALVAGSLLWNRAPEYAVLFSNLDERDGGQIIAELQQRNIPYRMSPTGHAIL
                     VPQAQVHETRLRLAADGLPKGSLAGFELMDGQKLGISQFNEQVNYQRALEGELTRTVQ
                     AIDAVASARVHLAMPKQTAFLRDDQRPTASVMVNLRGGRILSPDQVAGIVHLVSSSVP
                     RMHAEGVKIVDQNGKLLTEQADPLLRAGLNATQLEYVRLLEQGFIERIDKILAPLVGK
                     GNYGAQVAADVDFNQVEQTAETYKPNPTPDQAIRSQQTSEAFNPQPGAQGVPGALTNQ
                     PPVPATAPITNPQVAGGGAGAQGLASGNRSAVLNYELDRNIQHVKQAVGQIKRLSVAV
                     VVNNRTLPGPDGTPTNVPLPDEEIARITNLVREAVGYNADRGDTINVASGAFADDGSG
                     AAPPPWKDPEIVALGKEGLNWLLVLIAILFAYFGVIRPLLRTVVPPKPKEEKKGAAAG
                     EEGGEGEEGEEGVRVTLSGETGEGEETETFEQRLERARTAARNDPKMVANLIKDWMGM
                     NEEARK"
     gene            2615..3616
                     /gene="fliG"
                     /locus_tag="PKV97_11975"
     CDS             2615..3616
                     /gene="fliG"
                     /locus_tag="PKV97_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNB06642.1"
                     /translation="MTTPEEGLEKAALLLMTLGPDAAAGVLRQLGPREVQKLTMKMAS
                     MTPQSRETVEPVLIEVNEHFGKGSMIQSDDDQLREMLTKALGDDRANQLLSRMMSGSE
                     TAGIESLKWMDAATAADLIKGEHPQIIATILVHLEYDQAGEILKHFDDRLRNDVVLRI
                     ATLDGVQPTALKELNDALTRMLSGATTVKKAAMGGVRHAAEILNFVGSGAETAVIDNV
                     REYDPELAQKILDEMFVFENLLDIDDRGIQSILREVQSDSLIIALKGAPPELREKIFK
                     NMSSRAAEMLREDLEARGPVRLSEVEAEQKEILKTVRRLAEEGQVMLGGKGGDDGFV"
     gene            3731..4609
                     /locus_tag="PKV97_11980"
     CDS             3731..4609
                     /locus_tag="PKV97_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HNB06643.1"
                     /translation="MSISRHQAVGAYRRWEPEDFDAPAGKGSAPPPEAFAPADLAGLV
                     QAAPAPSTASPPIAPPAPEPAPSPPAADSVAALPPDFHLPTAEEIERMHDDMRRAGFE
                     EGRAAGHAEGLEAGRAEGYTAGRARAEAEAARLAGLADSLDEALRELDGEIAEQLMAL
                     AIEMARRMVKLTLAEHPEAILETVRSALLQLPQGHAHIQLHPDDLALVREHLGEQLAH
                     AGHRLQEDARLERGECRIDGQGAQVDATLETRWRRVLESIGHERARWHVAEEAEASDA
                     TGPVADTAPPAPEPPA"
     gene            4606..>5006
                     /locus_tag="PKV97_11985"
     CDS             4606..>5006
                     /locus_tag="PKV97_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HNB06644.1"
                     /translation="MNRHQALWRDFLGDGRRLVEGCNPLEVSGRLTRINGLVMEAAGI
                     KLPLGSGCRILVPGGGAVEAEVVGFNGDKLYMMPTDDVFGLAPGARVLPMEVGVPRLV
                     PGKRIGPRRRAADRAKHLPMEVGVPRLVPGKR"
BASE COUNT          831 a         1805 c         1775 g          595 t
ORIGIN      
        1 gagcagctcg cacaccgcga agcgctgacg tccggcgctg tcgccgcgcg cgaacagcag
       61 catgtcgcgg atgaggcgct cgaggtagcg caggcgttcg agcgcgcggt cggccaccat
      121 ggcgcgctgc gccgggtcga gctcgggctg gcgcaggttg ccggtgtaca gcagcgcggc
      181 ggcgagcggc gtgcgcaact ggtgggcgag cccggcgacc atctcgccca tggccgcgag
      241 gcgctcgttg cgctcggcgg tcaggcgcat gcggtgggtg tcggtgacgt cgtgaacgag
      301 caggatgcgc tcggcgccgg agtcgagctc ggactcggac agggccaagc ggcgcgggtc
      361 gccatcgacc gcggcagggt cgcggcgcag cacgaattcg cccggggtgc cgctgtcttc
      421 gaggcgggcg gcgagctccg cccacgccac cccggcgagc ccgcctccga acaagctctc
      481 ggcagcgcgg ttggcctgca ccacggtgcc ggcacgatcg accaccagca ccccggcggg
      541 cagcgccccc atcagcaccg acaggcgttc gctgagctgg ccgacctggg cctgcagcgc
      601 ggcgtaggcg gtggagagct cctcggaggc gcggctgaag agcgcgaagg cctcggcgag
      661 ctgggcggcg tcgagcaccg gcgccgcggc gggcatgctc cctgcttgat cggaggcgac
      721 gactgcggcc cgcatgggaa ggtccgaatc cgttttcacg ggatgcactc taaatgcaca
      781 ggccgccatg gtagccggca aacaggcggc aaaaaatgcc gcttttcacg ccacgaggct
      841 tgcacgcgcg gacgcacaat gccgccatcc gccaaagcgt atgcaacagg accgccatca
      901 tggccgcagc agaaaacacc gccgacctgc ctccgggcgc cgcccccgcc gatgcgcagg
      961 cgacgacgcg gatgcagcag gcgctgcaga acctcgccgc gctcagcacg cgccagaaga
     1021 tcgccggcgc ggtcgccatc gcgctggcga tcgcgctcgt ggccggctcc ctgctgtgga
     1081 accgcgcccc ggagtacgcg gtgctgttct ccaacctcga cgagcgcgac ggcggacaga
     1141 tcatcgccga gctgcagcag cgcaacatcc cctacaggat gtcgcccacc ggccacgcca
     1201 tcctggtgcc gcaggcgcag gtgcatgaga cgcggctgcg tctggcggcc gacggcctgc
     1261 ccaagggcag cctcgccggc ttcgagctga tggacggcca gaagctcggc atctcgcagt
     1321 tcaacgagca ggtgaactac cagcgcgcgc tcgaaggcga gctcacccgc accgtgcagg
     1381 cgatcgacgc ggtggccagc gcccgggtgc acctggcgat gcccaagcag accgccttcc
     1441 tgcgcgacga ccagcgcccc accgcctcgg tgatggtgaa cctgcgcggc ggccgcatcc
     1501 tctcgcccga ccaggtcgcc ggcatcgtgc acctggtgtc gtcgagcgtg ccacgcatgc
     1561 acgccgaggg cgtgaagatc gtggaccaga acggcaagct gctcaccgaa caggccgacc
     1621 cgctgctgcg cgcaggtctg aacgccaccc agctcgaata cgtccgcctg ctcgagcagg
     1681 gcttcatcga gcgcatcgac aagatcctcg cgcccctggt gggcaagggc aactacggcg
     1741 cccaggtggc ggccgatgtg gacttcaacc aggtcgaaca gacggccgag acctacaagc
     1801 ccaaccccac gcccgatcag gccatccgca gccagcagac cagcgaggcc ttcaacccgc
     1861 agccgggcgc gcagggcgtg ccgggcgcgc tcaccaacca gccgccggtg ccggccaccg
     1921 cgccgatcac caacccgcag gtggccggcg gcggcgcggg cgcgcagggc ctggcgagcg
     1981 gcaaccgcag cgcggtgctc aactacgagc tcgaccgcaa catccagcac gtcaagcagg
     2041 cggtcgggca gatcaagcgc ctgtcggtcg cggtggtggt gaataatcgc accctccccg
     2101 ggcccgacgg cacgcccacc aacgtgccgc tgcccgacga ggagatcgcg cgcatcacca
     2161 acctggtgcg cgaggcggtg ggctacaacg ccgaccgcgg cgacaccatc aacgtcgcca
     2221 gcggcgcctt cgccgatgac ggcagcggag ccgcaccgcc gccgtggaag gatccggaga
     2281 tcgtcgcgct cggcaaggaa ggcctgaact ggctgctggt tctgatcgcg atcctgttcg
     2341 cctactttgg cgtgatccgc ccgctgctgc gcaccgtggt gccgcccaag ccgaaggaag
     2401 agaagaaggg cgccgccgcg ggcgaggaag gcggggaagg cgaagagggc gaggaaggcg
     2461 tgcgcgtcac cctgtccggc gagaccggcg agggtgaaga gacggagacc ttcgagcagc
     2521 gtctcgagcg tgcccgcacc gcggcgcgca acgatcccaa gatggtcgcc aacctgatca
     2581 aggactggat gggcatgaac gaggaggcgc gcaagtgacg acacccgagg aaggcctgga
     2641 gaaggcggcg ctgctgctga tgaccctcgg ccccgacgcc gccgccgggg tgctgcgcca
     2701 gctcggcccg cgcgaggtgc agaagctgac catgaagatg gcctcgatga cgccgcagtc
     2761 gcgcgagacc gtggagccgg tgctgatcga ggtgaacgag cacttcggca agggctcgat
     2821 gatccagtcc gacgacgacc agctgcgcga gatgctcacc aaggcgctcg gcgacgaccg
     2881 cgccaaccag ctgctgtcgc gcatgatgag cggctcggag accgccggca tcgagagcct
     2941 gaagtggatg gacgcggcca ccgccgccga cctgatcaag ggcgagcacc cgcagatcat
     3001 cgccaccatc ctggtgcacc tcgagtacga ccaggccggc gagatcctca agcatttcga
     3061 cgaccgcctg cgcaacgacg tggtgctgcg catcgccacg ctcgacggcg tgcagccgac
     3121 cgcgctgaag gagctcaacg acgccctcac gcgcatgctc tcgggcgcga ccacggtgaa
     3181 gaaggcggcc atgggcgggg tacgccacgc cgccgagatc ctcaacttcg tcggttcggg
     3241 cgccgagacc gcggtcatcg acaacgtgcg cgaatacgac ccggagctgg cgcagaagat
     3301 cctcgacgag atgttcgtgt tcgagaacct gctcgacatc gacgaccgcg gcatccagtc
     3361 catcctgcgc gaggtgcagt ccgactcgct gatcatcgcg ctcaagggcg ccccgcccga
     3421 gctgcgcgag aagatcttca agaacatgtc ctcgcgcgcc gccgagatgc tgcgcgagga
     3481 cctcgaggcg cgcggcccgg tgcgactgtc cgaggtcgag gccgagcaga aggagatcct
     3541 caagacggtg cgccgcctgg ccgaggaagg ccaggtcatg ctcggcggca agggcgggga
     3601 cgatggcttc gtgtgatccc cgcgccgccc gcatgcggag ttcgcccgcg gcgcacccgt
     3661 gcggccgcgc gggaggaagc gcgtggcgcg gcccagggtg gagcccgcgt ggagacagga
     3721 ggatcgtccc gtgagcatct cgcgccacca ggcggtcggc gcctaccggc gctgggaacc
     3781 ggaggacttc gatgcccccg ccggcaaggg ctcggcgccg ccgccggaag ccttcgcgcc
     3841 cgcggatctc gcaggcctcg tgcaagcggc gcccgcgccc tccacagcct cgccgcccat
     3901 tgcccccccc gcgccggagc ccgccccgtc cccgcccgca gccgactctg tcgccgcgct
     3961 gccgcccgat ttccacctgc ccaccgcgga ggagatcgag cgcatgcacg acgacatgcg
     4021 ccgcgccggc ttcgaggaag gccgcgcggc cggccacgcc gaggggctgg aagccgggcg
     4081 tgccgaaggc tacaccgcgg gtcgtgcacg ggccgaggcc gaggctgcgc gcctggccgg
     4141 gctcgccgac agcctggacg aggccttgcg cgagctcgac ggcgagatcg ccgagcagct
     4201 gatggcgctg gcgatcgaga tggcgcggcg gatggtgaag ctcacccttg ccgagcaccc
     4261 cgaggccatc ctcgagacgg tgcgcagcgc gctgctccag ctgcctcagg gccacgccca
     4321 catccagctc cacccagacg acctcgccct ggtgcgcgag caccttggcg aacagctcgc
     4381 ccacgccggc caccgcctgc aggaggacgc ccgcctcgaa cgcggcgagt gccgcatcga
     4441 cggccagggc gcgcaggtgg atgcgacgct ggagacgcgc tggcggcggg tgctcgaatc
     4501 catcggccac gagcgcgcgc gctggcacgt tgcggaggaa gcggaggcga gcgacgcgac
     4561 cggcccggtt gccgacaccg ccccgcccgc gccggagccg ccggcatgaa ccgtcaccag
     4621 gcgctgtggc gcgacttcct cggcgacggc cgccgcctgg tcgagggctg caacccgctc
     4681 gaggtctccg gccgcctcac ccgcatcaac ggcctggtca tggaagcggc cggcatcaag
     4741 ctgccgctcg ggtcgggctg ccgcatcctg gtgcccggcg gtggcgcggt cgaggccgag
     4801 gtggtcggct tcaacggcga caagctctac atgatgccca ccgacgacgt cttcggcctg
     4861 gcgcccggcg cgcgcgtgct gccgatggaa gtcggtgtgc cgcgcctggt cccgggcaag
     4921 cgcatcggcc cgcgccggcg cgccgccgac cgcgccaagc acctgccgat ggaagtcggt
     4981 gtgccgcgcc tggtcccggg caagcg
//
LOCUS       DAOQNN010000241         4971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10242_length_4971_cov_9.947925, whole genome shotgun sequence.
ACCESSION   DAOQNN010000241 DAOQNN010000000
VERSION     DAOQNN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10242_length_4971_cov_9.947925"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PKV97_11990"
     CDS             <1..896
                     /locus_tag="PKV97_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB06645.1"
                     /translation="TDAASATCLNAREAAAFLQLNEKKLYELANSREIPAARVGGKWL
                     FPRALLEEWLLEQAHGGALSDRLLITGSDDPLLAATVGALAPVLGGDAFVAYSPTGTL
                     PGLELLARRRASVCALHWGGVEHSLQQHAMLLRRFAPHRHWALVRLALREQGVILRRG
                     LAVDGIETLAAFDYRWAMRQAGAGSAHFLESVLASRGFSAADCSVVATARSEREAAAL
                     VAREDADCAPGTRAAATEFGLGFLPLGWEAFDLALPRDVMFRRLFQDLLAAHGDARSQ
                     ALAHRLGGYDLGPLGRVLGLD"
     gene            complement(916..2250)
                     /locus_tag="PKV97_11995"
     CDS             complement(916..2250)
                     /locus_tag="PKV97_11995"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB06646.1"
                     /translation="MRIEKTTAGTPHRIVIVGGGAGGLELATRLGRKFGRGGRVEVTL
                     VDSKRTHVWKPKLHEIAAGSMDIGDHEVPYLAQAHWHGFRFRIGQMIGLDRSRREIHV
                     APFVDEDGKEVTPARSFPYDTLVIAVGSRSNDFGTPGVREHALKLETVADARRFHVRM
                     VNACVRAHAQRSPLRPEQLHVAIIGAGATGVELAAELHHTTREVVAYGLDRVDAEKDV
                     RLTLIEAAPRVLPALPERLSRSTERLLRKLGVEVNTDARVAEVLPDGVRLADGRVLPA
                     ELVVWAAGVKAPDFLTRLDGLETNRINQLVVRQTLQSSVDEDIFAMGDCAACPWPEKD
                     GFVPPRAQAAHQQASHLYTQLQRRLAGKPVQDYRYRDFGSLVSLGEFSTVGNMMGGLA
                     RGDLFIEGWFARLMYASLYKMHELALHGWVKVTLDTLARMITRRTEPHVKLH"
     gene            complement(2358..2849)
                     /gene="mobB"
                     /locus_tag="PKV97_12000"
     CDS             complement(2358..2849)
                     /gene="mobB"
                     /locus_tag="PKV97_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNB06647.1"
                     /translation="MKVMGFAGWSGSGKTTLVEQVIAALSARGLVVSLVKHAHHSFDI
                     DHAGKDSWRHRQAGCREVMVSSDQRWSLTRELRGGPEATLDELLAHLGPCDLVLVEGF
                     KRAAIPKIEVHRRGLAEPLLYPHDPHIVAIATDAELPATLPRLDLNDPVQVADFIADH
                     LHL"
     gene            complement(2846..4927)
                     /locus_tag="PKV97_12005"
     CDS             complement(2846..4927)
                     /locus_tag="PKV97_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNB06648.1"
                     /translation="MRLAERLRGSVRAKLLALVLAPLMLGFPLIMGVLWYWGEVYYHR
                     LMVSRVASDLATAHGYFERVIDGVGNGVQGLAGSHALAQTLAHESPEAVAALLGARKG
                     GLALDFLNLLDVNGRVLHASTALAAGSARGGWPVVQQAIRARGGSVVERFAPAELAAI
                     DAGMRERARLALVATARARPDSRGEEERGLVVHAAAPVLDAAGNLVAVLEGGVLLNGN
                     LDFVDTLNAIVYREGSLPEGSEGTATLFVDDVRIATNVRLFEGARALGTRASDEVRTH
                     VLDHGRTWLETAFVVNDWYVSAYEPVVDSRGERVGMLYVGFLEAPFRAAKRMALLVVG
                     ALALLVSAAGALLTLYWARGIFRPIERMHATIGRIDAGDEQARVGDVASRDELGRLAT
                     AFDHLLDDQAARRTELQALNASLDRKVAERTADLADANAELRAAQHRLVMSEKLAAIG
                     ELTAGVAHEISNPTAVIQGNLDLLREELGPAAQPVANEIRLIHEQVGRIRLIVTKLLQ
                     FARPGEFAGYVEDVDVNAALADCLVLTRQHLARAEVKVVQRLAATARVQINLQELQQV
                     LINLIVNAVQAMPAGGTLTLETVDRDPRQDARGVRITVRDTGGGIRAEDLARIFDPFF
                     TTKKRQGTGLGLSISHTLVERYGGRIEVDSAPGRGAAFTVSLLAEPVYRKDPAPPPGG
                     RENGTGQTEAT"
BASE COUNT          705 a         1804 c         1735 g          727 t
ORIGIN      
        1 gcaccgacgc ggcatccgcg acctgcctca acgcacgcga ggccgcggcc ttcctgcagc
       61 tcaacgagaa gaagctctac gaactcgcca acagccgcga gattcccgcc gcccgcgtgg
      121 gcggcaagtg gctgttcccg cgcgcactgc tcgaggagtg gctgctcgag caggcgcatg
      181 gcggcgcact cagcgaccgg ctgttgatca ccggcagcga cgacccactg ctggccgcca
      241 cggtgggcgc gctcgctccc gtgctggggg gcgatgcctt cgtcgcctac agccccaccg
      301 gcaccctgcc cgggctcgag ctgctcgcgc gtcgccgcgc cagcgtgtgt gcgctgcact
      361 ggggcggggt ggagcacagc ctgcagcagc acgccatgct gctgcgccgc ttcgccccgc
      421 atcgccattg ggcgctggtg cgcctggcgc tgcgcgagca gggcgtaatc ctgcgccgcg
      481 gcctggcggt cgacggcatc gagacgctcg ccgccttcga ctatcgctgg gcgatgcgcc
      541 aggccggcgc gggttcggcg cacttcctcg agtccgtgct cgccagccgc ggtttctcgg
      601 cggccgactg cagcgtggtc gccaccgccc gcagcgagcg cgaggccgcg gccctggtcg
      661 cacgcgagga cgccgactgc gcgcccggca cgcgcgccgc agcgaccgag ttcggcctcg
      721 gcttcctgcc cttgggctgg gaggccttcg acctcgcgct gccgcgcgac gtgatgttcc
      781 gccgcctctt ccaggatctg ctcgccgcgc acggcgacgc gcgctcacag gcgctggcgc
      841 accggctcgg cggctacgac ctcggcccgc tcgggcgcgt gctcggcctc gactgaaggc
      901 gcggcacgcg cggcctcagt gcagcttgac gtggggctcg gtgcggcgcg tgatcatgcg
      961 cgccaaggtg tcgagcgtca ccttcaccca gccgtgcagc gccagttcgt gcatcttgta
     1021 caaggaggca tacatcaggc gcgcgaacca gccctcgatg aacaggtcgc ccctggcgag
     1081 cccgcccatc atgttgccca ccgtgctgaa ctcgcccagc gacaccagcg aaccgaagtc
     1141 ccggtagcgg tagtcctgca ccggcttgcc ggcgaggcgg cgctgcaact gcgtgtacag
     1201 gtgcgaggcc tgctggtgcg cggcctgcgc gcgcgggggc acgaagccgt ccttttccgg
     1261 ccacgggcag gcggcgcagt cgcccatcgc gaagatgtcc tcgtctaccg aactctgcag
     1321 ggtctgccgc accacgagct ggttgatgcg attggtctcc agcccgtcga gccgggtgag
     1381 gaaatcgggc gccttcaccc cggccgccca caccaccagc tcggcgggca ggacgcggcc
     1441 gtcggccagg cgcacgccat cgggcagcac ctcggccacg cgtgcgtcgg tattcacttc
     1501 cacacccagc ttgcgcagca agcgctcggt cgatcgcgac aggcgctcgg gcaaggccgg
     1561 cagcacgcgc ggcgcggctt cgatcagggt gagccggacg tccttttcgg catcgacgcg
     1621 gtccagcccg tacgccacca cctcgcgcgt ggtgtggtgc aactcggcgg caagctcgac
     1681 gccggtggcg ccggcaccga tgatcgccac atgcaactgc tcggggcgca gcggcgaacg
     1741 ctgggcgtgg gcgcgcacgc acgcattgac catgcgcaca tggaaacgcc gcgcatcggc
     1801 gacggtctcg agcttgagcg catgctcgcg cacccccggc gtgccgaaat cgttgctgcg
     1861 gctgccgacc gcgatcacca gcgtgtcgta ggggaaggaa cgcgcgggcg tgacctcctt
     1921 gccgtcttca tcgacgaagg gggcgacgtg gatctcgcgc cggctgcggt cgagcccgat
     1981 catctgcccg atgcggaagc ggaagccatg ccagtgcgcc tgcgccaggt agggcacctc
     2041 gtgatcgccg atgtccatgc tgccggcagc gatctcgtgg agcttgggct tccacacgtg
     2101 ggtgcgcttg ctgtcgacga gggtgacctc gacccgcccg ccgcggccga acttgcgccc
     2161 caggcgtgtc gccagctcca gtccgccggc gcccccgccg acaatgacga tgcggtgggg
     2221 cgtccccgcc gtggtctttt cgatcctcat taccgtctct cgagaaaatt attaatctcg
     2281 agaatcctaa cagagcattc gccctgccat accgggcgtt cgcatggatg caacagactg
     2341 gaccgacctc gcccgcacta caggtgcaga tggtccgcga tgaagtcggc cacctgcacc
     2401 gggtcgttga ggtccagccg cggcagcgtc gcaggcagct cggcgtcggt cgcgatggcg
     2461 acgatgtgcg gatcgtgcgg atacagcagc ggctcggcga gcccccggcg atgcacctcg
     2521 atcttgggga tcgccgcgcg cttgaagccc tcgaccaaca ccagatcgca cggtccgagg
     2581 tgggcaagca gctcgtcgag cgtcgcctcg ggcccgccgc gcagctcgcg tgtcagcgac
     2641 cagcgctggt ccgagctcac catgacctcg cggcagccgg cctggcgatg gcgccaggaa
     2701 tccttgccgg catggtcgat gtcgaagctg tggtgggcgt gcttgaccag cgacaccacc
     2761 aggccgcgcg cgctcagcgc cgcgatcacc tgctccacca gcgtcgtctt gccgctgccg
     2821 gaccagccgg cgaaacccat cactttcatg tcgcttccgt ttgtcctgtt ccgttctccc
     2881 gtccgccggg cggcggcgcg gggtccttgc gatacaccgg ctcggcgagc aggctcaccg
     2941 tgaaggcagc gccccgcccg ggcgcgctgt ccacctcgat gcggccaccg tagcgctcga
     3001 ccagggtgtg gctgatcgac agccccagcc cggtgccttg gcgcttcttg gtggtaaaga
     3061 agggatcgaa gatgcgcgcc aggtcctcgg cacggatgcc gccgccggtg tcgcgcaccg
     3121 tgatgcgcac gccgcgcgca tcctgccgcg ggtcgcggtc gaccgtctcc agcgtgagcg
     3181 tgccgccggc cggcatggcc tgcacggcgt tgacgatgag gttgatcagc acctgctgca
     3241 gctcctgcag gttgatctgc acccgcgcgg tggcggccag gcgctgcacc accttcacct
     3301 cggcgcgcgc cagatgctgg cgcgtcagca ccaggcagtc ggcgagcgcg gcgttcacgt
     3361 cgacgtcctc gacgtagccg gcgaactcgc ccggacgggc gaactgcagc agcttggtga
     3421 cgatcaggcg aatgcgcccg acctgctcgt ggatcaggcg gatctcgttg gccaccggct
     3481 gcgccgcggg gccgagctcc tcgcgcagca ggtcgagatt gccctggatc accgcggtgg
     3541 gattgctgat ctcgtgcgca acgccggcgg tgagctcgcc gatcgcggcg agcttctcgc
     3601 tcatcaccag gcggtgctgt gcggcgcgca gctcggcgtt ggcgtcggcg aggtcggcgg
     3661 tgcgctcggc gaccttgcgg tcgagcgaag cgttgagcgc ctgcagctcg gtgcggcgcg
     3721 cggcctggtc gtcgagcagg tggtcgaagg cggtagccag gcggccgagc tcgtcgcggc
     3781 tggcgacgtc acccacgcgc gcctgctcgt cgccggcgtc gatgcggccg atggtggcgt
     3841 gcatgcgctc gatcgggcgg aagatgccgc gcgcccaata cagcgtcagc agcgcgccgg
     3901 cggcgctgac cagcagcgcg agcgcgccga ccaccagcag ggccatgcgc ttggcggcgc
     3961 ggaagggcgc ctcgaggaag ccgacgtaga gcatgccgac gcgttcgcca cggctgtcca
     4021 ccaccggctc gtaagccgac acgtaccagt cgttgaccac gaaggcggtc tccagccagg
     4081 tacgcccgtg gtcgagcaca tgcgtgcgga cctcgtccga ggcgcgcgtg ccgagcgcgc
     4141 gcgcgccctc gaacaggcgc acgttggtgg cgatgcgcac atcgtcgacg aagagcgtgg
     4201 cggtgccctc gctgccctcg ggcaggctgc cctcgcggta gacgatggcg ttgagggtat
     4261 cgacgaagtc gaggttgcca ttgagcagca cgccgccttc gagcaccgcg accaggttgc
     4321 ccgcggcgtc gagcaccggg gccgccgcgt gcaccaccag gccgcgctcc tcctcgccgc
     4381 gcgaatccgg ccgcgcgcgc gccgtggcca ccagggcaag gcgggcacgc tcacgcatgc
     4441 cggcgtcgat cgccgcgagc tcggcgggtg cgaagcgctc gacgacgctg ccgccgcggg
     4501 cgcggatcgc ctgctgcacg acgggccatc caccacgcgc gctccccgcc gccagcgcgg
     4561 tcgacgcatg cagcacgcgg ccattgacgt cgagcaggtt gaggaagtcg agcgcgagtc
     4621 cgcccttgcg cgcaccgagc agcgcggcca ccgcttccgg actctcgtgc gccagggtct
     4681 gcgcgagcgc atgcgagccg gcgagcccct gcacgccgtt gccgacgccg tcgatcacgc
     4741 gctcgaagta accgtgcgcg gtcgccaggt cgctggcgac ccgcgacacc atcaggcggt
     4801 ggtagtagac ctcgccccag taccacagca cgcccatgat cagcgggaag cccagcatca
     4861 gcggcgcgag cacgagggcg agcagcttgg cgcgtaccga gccgcgcagg cgttcggcga
     4921 gcctcatcga cccgctcccg caccgggccc cgccatccgg cgcggcctgc t
//
LOCUS       DAOQNN010000242         4947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10325_length_4947_cov_7.581153, whole genome shotgun sequence.
ACCESSION   DAOQNN010000242 DAOQNN010000000
VERSION     DAOQNN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10325_length_4947_cov_7.581153"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKV97_12010"
     CDS             <1..447
                     /locus_tag="PKV97_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HNB06649.1"
                     /translation="QIGIAVPILPPAGALLGFLDADAAPVEWLGHANALLHTAACLIE
                     HRLIETEPRGFLLLRFHRYASVLGTPLEALVLFDAEGAVLLANHTARALLAPCAFGAG
                     GAAACFATQWCGIVGYAALGLCEPFVLRDRRGGGFSAQASLRRQAT"
     gene            444..1850
                     /locus_tag="PKV97_12015"
     CDS             444..1850
                     /locus_tag="PKV97_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNB06650.1"
                     /translation="MSGARTPSLQARLSLVLTVLIAVLMSAGAAVWMRETRQAIHEEV
                     EAATRVAQQWLVVVLAEQRDGDAEARARLLSRLAAVGRIRANRLEVFGAGGERLYVSP
                     ESPYKAGRDAPAWFAQHVAPELGARRLDAGALEVVLTPDTSRAVLDAWDHLVAALGWA
                     CALLLALWLATRVAIRRALAPLQQIHVALEHGARGRFDQRLPEYATRELALLARSYNR
                     LADRLDESRATNARLEADQSLARTIQARLEQERRAIARELHDELGQSITAVRSIAGAI
                     LQRSAEQPGIHGSAQAILAMTGHMQDGVHAILQRLRRDAPAAHERIEDTLQAYCALWA
                     SHHEGIALRCQIEPLGEARDEPLDDGVGLAVLRVLQESLTNVARHARARSVEVRVGLR
                     REGGGRDGQAEIEVEVRDDGRGLGPQPTPGRHGLTGMRERLAELHGRLELHSPPTGGL
                     CVRARLPQALRQPLPVTQ"
     gene            1850..2500
                     /locus_tag="PKV97_12020"
     CDS             1850..2500
                     /locus_tag="PKV97_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB06651.1"
                     /translation="MKPPSLHALRILLADDHAVVRMGFRLLLEGAGAEVVGEADCGEA
                     AVQAFGQLHPDVLLMDVSMPGIGGLGALERVLAHHPGARVLMLSAHDDAQIPARALRA
                     GAGGYLSKRAHPDELLHAVATVARGQRYIDPAIAPALALAQFAGGGDPVAALTEKEFA
                     VFLQLARGRSVAEIAETHSLSPSTVGTHLYHIKQKLEVSNAAELALVAVRCGLIEV"
     gene            2630..3310
                     /locus_tag="PKV97_12025"
     CDS             2630..3310
                     /locus_tag="PKV97_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNB06652.1"
                     /translation="MSKEKDPQDFLAPSYYFDHEHPRVRAFIEESVGDAVDAVERAVK
                     LYYAVRDGIRYDPYRFYMRKEYFTASRTLIEGAAYCVPKANLLTAVARGAGIPARVGF
                     ADVRNHLSTERLRQLVGGDVYRWHGYVAMHLEGRWVKATPAFNLQMCERFDVHPLEFD
                     GRNDSLMHPYNARDERHMEYVKERGTFDDFPFEALVADMWEHHPGLVEAAEADLKGDF
                     EHEAMVDR"
     gene            complement(3392..3934)
                     /locus_tag="PKV97_12030"
     CDS             complement(3392..3934)
                     /locus_tag="PKV97_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06653.1"
                     /translation="MPVDLLFAMLRNPASTGAVLPSSRTLANAMAGAALGADTVIELG
                     AGTGPVTEALLRRLPGVPLIAVELQPALARRLQARFPTVDVRQAAAKEVVDGLIERPG
                     RLVLVSSLPFRSLPKEVAAATVDSLCRFLAHDGERKLVQFTYQPRAPFAVPRHLRWQR
                     RTVVWRNTPPAGVWELQAVR"
     gene            4046..>4947
                     /locus_tag="PKV97_12035"
     CDS             4046..>4947
                     /locus_tag="PKV97_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HNB06654.1"
                     /translation="MAGTTLLALIDDIATLLDDVATMTKVAAKKTAGVLGDDLALNAQ
                     QVTGVQASRELPVVWAVAKGSLVNKAILVPAALLISWLAPWLITPLLMLGGAFLCFEG
                     FEKLAHKFLHSKAEDEAEHRETLEAVADPNVDLVAYEKEKIKGAVRTDFILSAEIVVI
                     TLGTVAAASLGQRVTVLVGISLVMTVGVYGLVAGIVKLDDLGLHLQRSASAFTRRIGG
                     AILGFAPWLMKTLSVVGTAAMFMVGGGILLHGWPTAAHAVEHFAAGFGGVGGALINTG
                     AGAGVGIVAGAIVLAAVMVVQKLRG"
BASE COUNT          704 a         1858 c         1727 g          658 t
ORIGIN      
        1 cagatcggca tcgcggtgcc gatcctgccc ccggccggcg ccctgctcgg cttcctcgac
       61 gccgacgccg ccccggtgga atggctgggc cacgccaacg cgctgctcca caccgccgcc
      121 tgcctcatcg agcaccgcct catcgagacc gagccgcggg gcttcctgct gctgcgcttc
      181 caccgctacg cgagcgtgct cggcaccccg ctcgaagcgc tcgtgctgtt cgatgccgaa
      241 ggcgcggtcc tgctcgccaa ccacaccgcg cgcgccctgc tcgcgccctg cgccttcggc
      301 gcgggcggcg ccgcggcctg cttcgccacc cagtggtgcg gcatcgtcgg ctacgccgcg
      361 ctcggcctgt gcgagccctt cgtgctgcgc gaccggcgcg gcggcggctt ctccgcgcag
      421 gccagcctgc gccggcaggc gacatgagcg gcgcgcgcac gcccagcctg caggcccgcc
      481 tcagcctggt gctcaccgtg ctgatcgcgg tgctgatgag tgcgggcgcg gccgtgtgga
      541 tgcgcgagac gcgccaggcc atccacgaag aggtcgaggc cgccacccgg gtcgcgcagc
      601 agtggctggt cgtggtgctg gcggagcagc gcgacggcga cgccgaggca cgcgcgcggc
      661 tgctgagccg gctcgccgcg gtcgggcgca tccgcgccaa ccggctcgaa gtcttcggcg
      721 cgggcggaga acggctgtac gtgtcgcccg agtcgcccta caaggccggt cgcgacgcgc
      781 cggcctggtt cgcgcagcac gtcgcgcccg agctcggcgc gcgccggctc gacgccggcg
      841 cgctggaggt ggtgctgacg cccgacacct cgcgcgcggt gctcgacgcc tgggaccatc
      901 tcgtcgccgc gctcggctgg gcgtgcgcgc tgctgctcgc gctctggctc gccacccgcg
      961 tggcgatccg gcgcgcgctc gcgccgctgc agcagatcca cgtcgcgctc gagcacggcg
     1021 cccgcggccg cttcgaccag cgcctgccgg aatacgccac ccgcgagctc gcgctcctcg
     1081 cgcgcagcta caaccgcctc gccgaccgcc tggacgagtc gcgcgccacc aacgcccgcc
     1141 tcgaggccga ccagagcctc gcgcgcacca tccaggcccg gctggagcag gagcgccgcg
     1201 cgatcgcgcg cgagctccac gacgagctcg gccagagcat caccgcggtg cgctcgatcg
     1261 ccggcgccat cctgcagcgc agcgccgagc agcccggcat ccacggcagc gcgcaggcca
     1321 tcctcgccat gaccggccac atgcaggacg gcgtgcacgc gatcctgcag cgcctgcgcc
     1381 gcgacgcacc ggccgcgcac gagcgcatcg aggacacgct gcaggcctac tgcgcgctgt
     1441 gggccagcca tcacgaaggc atcgcgctgc gttgccagat cgagcccctc ggcgaggctc
     1501 gcgacgagcc gctcgacgac ggcgtcggcc tcgccgtgct gcgcgtgctg caggagagcc
     1561 tcaccaacgt cgcccgccac gctcgcgcgc gctcggtcga ggtgcgcgtc ggcctgcgcc
     1621 gcgagggcgg cggtcgcgac ggacaggcgg agatcgaggt cgaggtccgc gacgacggcc
     1681 gcggcctggg cccgcagccc acccccggcc gccacggcct caccggcatg cgcgagcgcc
     1741 tcgccgaact ccacggccgg ctcgaactgc acagcccgcc caccggcggc ctgtgcgtgc
     1801 gcgcccgtct cccgcaggct ctgcggcaac ccctaccggt cacgcaatga tgaagccccc
     1861 ttccctccac gccctgcgca tcctgctcgc cgacgatcat gcggtggtgc gcatgggctt
     1921 ccgcctgctg ctcgaaggcg ccggtgccga ggtcgtcggc gaggccgatt gcggcgaggc
     1981 ggccgtgcag gccttcggcc agctgcaccc cgatgtgctg ctgatggacg tgtcgatgcc
     2041 gggcatcggc ggactcggcg cgctcgagcg cgtgctcgcc caccaccccg gggcgcgcgt
     2101 gctgatgctg tcggcgcacg acgacgcgca gatccccgcg cgcgcgctgc gtgccggcgc
     2161 cggcggctac ctgtccaagc gcgcccatcc ggacgagttg ctgcacgcgg tggccaccgt
     2221 cgcgcgcggc cagcgctaca tcgacccggc gatcgcgccc gccctcgcgc tcgcgcagtt
     2281 cgccggcggc ggcgacccgg tcgcggcact caccgagaag gaattcgccg tcttcctgca
     2341 actggcgcgc gggcgcagcg tggccgagat cgccgagacc cacagcctga gcccgagcac
     2401 ggtgggcacc cacctgtatc acatcaagca gaagctcgaa gtgtcgaatg ccgccgagct
     2461 cgcgctcgtc gcggtgcgct gcggcctcat cgaagtctag cgacaggcgc gtcggcgccc
     2521 gcggagcaga catcgcggcg atccgtgcgg cgcaaataat attgacgcga acgtcaactt
     2581 ccccttatac attcccgaga caacgcaggc acaccgagga gaaccgggaa tgagcaagga
     2641 gaaggatccg caggactttc tggcaccgag ttattacttc gaccacgagc atccgcgcgt
     2701 gcgcgccttc atcgaggaga gcgtgggcga cgcggtcgat gcggtcgagc gcgcggtgaa
     2761 gctgtactac gcggtgcgcg acggcatccg ctacgacccc taccgcttct acatgcgcaa
     2821 ggagtacttc accgccagcc gcacgctgat cgagggcgcc gcctactgcg tgcccaaggc
     2881 caacctgctc accgccgtcg cacgcggcgc gggcatcccg gcgcgggtgg gcttcgccga
     2941 cgtgcgcaac cacctcagca ccgagcgcct gcgccagctc gtcggcggcg acgtgtatcg
     3001 ctggcacggc tacgtcgcca tgcacctgga aggccgctgg gtgaaggcga cgccggcgtt
     3061 caacctgcag atgtgcgagc gcttcgacgt ccatccgctc gaattcgacg gccgcaacga
     3121 ctcgctgatg cacccctaca acgcgcgcga cgaacgccac atggagtacg tgaaggagcg
     3181 cgggaccttc gacgacttcc ccttcgaggc gctcgtcgcc gacatgtggg agcaccaccc
     3241 gggtctggtc gaagcggccg aggccgacct gaagggcgac ttcgagcacg aggccatggt
     3301 cgaccgctga gcggccaccg cgcgagcacc gccgggcggc ggcgtggcgg acacggcacg
     3361 gcttcacggg tgacttgccg ccgcgaaggc ctcagcgcac cgcctgcagc tcccacaccc
     3421 ccgccggcgg cgtgttgcgc cacaccacgg tgcggcgctg ccagcgcagg tggcgcggca
     3481 ccgcgaacgg cgcgcgcggc tggtaggtga actgcacgag cttgcgctcg ccgtcgtggg
     3541 cgaggaatcg gcagaggctg tcgaccgtcg ccgccgcgac ctccttgggc agggagcgga
     3601 aaggcaggct ggaaaccagc accaggcggc cggggcgctc gatcaggcca tcgacgacct
     3661 ccttcgccgc cgcctggcgc acatcgaccg tcggaaagcg cgcctgcagc cgccgcgcca
     3721 gcgcgggctg gagctcgacc gcgatcagcg gcacgccggg caggcggcgc agcaaggcct
     3781 cggtcaccgg gccggtgccg gcgccgagct cgattacggt gtcggcgccg agcgccgcgc
     3841 ccgccatcgc attggccagc gtgcgcgagg aaggcagcac cgcgccggtc gatgcggggt
     3901 tgcgcagcat ggcgaacagc agatcgaccg gcatcggccc cccttctcaa actcgcaagg
     3961 cgcccatggt agcgccatgc ggacctgccc ggcacccggc ggatttctgt caccatcacg
     4021 cctttcctcc ccacaacggt agatcatggc cggaaccacc ctgctcgcac tcatcgacga
     4081 catcgccacc ctcctggacg acgtcgccac catgaccaaa gtcgccgcca agaagaccgc
     4141 cggcgtgctc ggcgacgacc tcgccctcaa cgcccagcag gtcaccggcg tgcaggccag
     4201 ccgcgagctg ccggtggtgt gggcggtggc caagggctcg ctggtgaaca aggccatcct
     4261 ggtgccggcg gcgctgctga tcagctggct ggcgccgtgg ctgatcaccc cgctgctgat
     4321 gctcggcggc gccttcctgt gcttcgaggg cttcgagaag ctcgcgcaca agttcctgca
     4381 ctcgaaggcg gaggacgagg ccgagcaccg cgagaccctg gaggcggtgg ccgaccccaa
     4441 cgtggacctg gtcgcctacg agaaggagaa gatcaagggc gcggtgcgca ccgacttcat
     4501 cctgtcggcc gagatcgtgg tgatcaccct cggcaccgtg gccgccgcct cgctcggcca
     4561 gcgcgtgacg gtgctggtcg gcatctcgct ggtgatgacg gtgggcgtgt atggcctggt
     4621 ggccggcatc gtcaagctcg acgacctcgg cctgcacctg cagcgcagcg cctcggcctt
     4681 cacccgccgc atcggcggcg ccatcctggg cttcgcaccg tggctgatga agacgctgtc
     4741 ggtggtcggc accgcggcga tgttcatggt cggcggcggc atcctgctgc acggctggcc
     4801 gacggcggcg cacgcggtgg agcatttcgc cgcgggtttc ggcggcgtgg gcggcgcatt
     4861 gatcaacacc ggcgcgggcg cgggggtcgg catcgtcgcc ggtgcgatcg tgctcgcggc
     4921 ggtgatggtg gtgcagaagc tgcgcgg
//
LOCUS       DAOQNN010000243         4918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10422_length_4918_cov_11.076702, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000243 DAOQNN010000000
VERSION     DAOQNN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10422_length_4918_cov_11.076702"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKV97_12040"
     CDS             complement(<1..374)
                     /locus_tag="PKV97_12040"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNB06655.1"
                     /translation="MSSPLYDLEVDRLAGGTTTLGEYAGKLLLIVNTASQCGLTPHYA
                     GLEKLYQTYKDRGLVVLGFPCNQFGAQEPGSAEEIGAFCARNYGVSFPMFAKIEVNGD
                     GAHPLYKYLKQHAKGILGTEAI"
     gene            complement(480..2852)
                     /locus_tag="PKV97_12045"
     CDS             complement(480..2852)
                     /locus_tag="PKV97_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB06656.1"
                     /translation="MTRAAFALLLHTSSSIRLLVLALIGLAGLGWFVVEAGTSSAREH
                     LRYARDLREIRQVDAELGAAALARRLGLAEDWHGADAHLARMGELLEDLGRVPDFLTG
                     SEMAAVRRQLDHYTALQTDKRTVISALRVLLDAPPQNGGAAEAEADRLLRQLLALQTA
                     REGERLVESYTDGYVASQTRMHRHRVLLVLAALALGAYLLLTALRLRAVSLTVSKAHA
                     DLQEHAAALGRAEAELRLYATVFTNASEGMLITDAHSRIIAANPAFSRITGYSPAEVA
                     GRSPALLNSGRQDAGFYRRMWSTLASRGKWQGELWNRRRDGSIYPEWLSITAVPGEGE
                     GADGQPCNYIGIFSDITERKEAEARILHLAHHDPLTNLPNRALLNDRLSQSLLQARRD
                     GRGAAVLLLDLDRFKTINDTLGHERGDSLLLEICARCRRVLRDTDTLARLSGDEFVIV
                     LPDATTADVSADTARRILDAIALPCRLGEHEISITASIGIALFPRDGGDESTLLRNAD
                     AAMHRAKEAGRNGFEFYTEDMNTSSLGRLLLEHQLRGAAERGELLLHYQPKVSAAGGR
                     LGGFEALLRWQHPELGMVAPGQFIPLAEESGLIVPIGQWVLEEACRQQRAWLDAGFDV
                     PPLAVNLSAHQLAQPDIVATVAAAIECHRLAPGLIELELTETMLMRDVDRTIRTLVRL
                     REMGVRLAIDDFGTGYSSLNYLRQFPVNALKIDRSFVSDIGSDGAEGKIAAAIVGMAH
                     SLGLDAVAEGVESEAQRAFLQMHGCHQLQGFLIARPAPAREIERFLSRRA"
     gene            complement(3052..3828)
                     /locus_tag="PKV97_12050"
     CDS             complement(3052..3828)
                     /locus_tag="PKV97_12050"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HNB06657.1"
                     /translation="MSSLATERVLSVHHWNESLFSFRTTRDPGLRFENGQFVMIGLDV
                     GGKPLTRAYSIASPNYEEHLEFFSIKVPDGPLTSRLQHLRPGDPIVVSKKPTGTLVLH
                     DLNPGKHLYLLATGTGLAPFLSVVQDPETYERFEKVVLVHGVRFVSELAYTDFITREL
                     PQHEYFGEQVREQLIYYPTVTREPFRNTGRITHVIDSGRLFADIGLPELDPGHDRVMI
                     CGSQAMNKDCCDLLDARGFKMSPRIGVAGDYVIERAFVEK"
     gene            3936..4862
                     /locus_tag="PKV97_12055"
     CDS             3936..4862
                     /locus_tag="PKV97_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB06658.1"
                     /translation="MKFTLRQIQVFVAVARAEHVSRAAEGLALSQSAASAALAELESQ
                     CAQPLFDRHGKRLRLNEQGSLLLPHAVELLDRAAEFEGRMRGEQGLGPLRVGATLTIG
                     NYLLPLIVSDFLQAHPESRVHLQVRNTATIAERLLHHEIDLGLVEGQVVDAELHVEPW
                     VEDELVVFCAPGHALAARGRASVAELAGQPWILRERGSGTRETFDRALRHHPGGVVPR
                     LELEHTEAIKRAVESGLGLGCLSRLALRDAFRRGSLVAVEVPELDLRRRFSFVWHRGK
                     YHGAALRRFVEDCRAFTAGARRSDEIPLPPVA"
BASE COUNT          688 a         1557 c         1845 g          828 t
ORIGIN      
        1 ttgatcgcct cggtgccgag gatgcctttc gcgtgctgtt tcagatactt gtacagcgga
       61 tgtgcgccgt cgccgttgac ctcgatcttg gcgaacatcg gaaagctcac gccgtagttc
      121 ctggcgcaga aggcgccgat ctcctccgcg ctacccggct cctgcgcgcc gaactggttg
      181 cacggaaagc cgagcaccac gaggccgcgg tccttgtagg tctgatacag cttttccagg
      241 ccggcgtaat gcggggtgag cccgcactgg cttgcggtgt tgacgatcag cagcagcttg
      301 ccggcgtatt cgccgagcgt ggtggtgccg ccggcgaggc ggtcgacttc gaggtcgtac
      361 aggggcgagc tcatggggga gggtctccgt gcgcgggtgg gatgcgggcc cgccgcatgg
      421 cggacttcgc aggcttggag gcgggcgggc cggcgcaagt ttccgccgag ccgcgcgatt
      481 caggcgcgcc ggctcaggaa gcgctcgatt tcgcgcgcag gcgccgggcg ggcgatcagg
      541 aagccctgca actgatggca gccgtgcatc tgcaggaagg cgcgttgggc ctcgctctcc
      601 acgccttcgg cgaccgcgtc cagccccagg ctgtgggcca tgccgacgat ggctgcggcg
      661 atcttgccct cggcgccgtc gctgccgatg tcgctgacga agctgcggtc gatcttcagc
      721 gcattgaccg ggaactggcg caggtagttg agcgaggagt agccggtgcc gaagtcgtcg
      781 atcgccaggc gcacgcccat ctcgcgcagg cgcacgaggg tgcggatggt gcgatcgacg
      841 tcgcgcatca gcatggtctc ggtgagctcg agctcgatca ggccgggcgc gaggcggtgg
      901 cactcgatgg ccgccgccac ggttgcgacg atgtcgggtt gggcgagctg gtgcgccgag
      961 aggttcaccg cgagcggcgg cacgtcgaaa ccggcgtcca gccaggcgcg ctgttggcgg
     1021 caggcttcct cgagcaccca ctggccgatc ggcacgatca ggcccgattc ttcggcgagc
     1081 gggatgaact gccccggggc caccatgccg agctcgggat gctgccagcg cagcagcgcc
     1141 tcgaagccgc cgaggcggcc gccggcggcg ctcaccttcg gctggtagtg cagcagcagt
     1201 tcgccgcgct cggcggcgcc gcgcagctgg tgttcgagca acaggcggcc gagcgaactg
     1261 gtgttcatgt cctcggtgta gaactcgaaa ccgttgcgcc cggcctcctt ggcgcgatgc
     1321 atggcggcgt cggcattgcg caacagggtc gattcgtcgc cgccgtcgcg cgggaagagg
     1381 gcgatgccga tgctcgccgt gatgctgatc tcgtgctcgc ccagccggca gggcagggcg
     1441 atggcgtcga ggatcctgcg cgcggtgtct gcgctgacgt cggcggtggt ggcgtcgggc
     1501 agcacgatga cgaactcgtc cccgctcagg cgggcaagcg tgtcggtgtc gcgcagcacg
     1561 cgccggcagc gtgcgcagat ctccagcagc agactgtccc cgcgttcgtg gccgagggtg
     1621 tcgttgatcg tcttgaagcg gtcgaggtcg agcagcagca ccgcggcgcc gcggccgtcg
     1681 cgccgtgcct gcagcaggct ctggctcagg cggtcgttga gcagcgcgcg gttgggcagg
     1741 ttggtgagcg ggtcgtggtg ggcgaggtgc aggatgcgcg cttcggcctc cttgcgttcg
     1801 gtgatgtcgg agaagatgcc gatgtagttg cacggctgtc cgtccgcccc ctcgccctcg
     1861 cccggcaccg cggtgatcga cagccactcg ggatagatcg agccgtcgcg gcggcggttc
     1921 cacagttcgc cctgccactt gccgcggctg gcgagcgtgg accacatgcg ccggtagaag
     1981 ccggcgtcct gccgcccgga gttcagcagc gccggcgagc gccccgcgac ctccgccggg
     2041 ctgtagccgg tgatgcggga gaaggccggg ttggcggcga tgatgcggct gtgcgcatcg
     2101 gtgatcagca tgccttcgct ggcgttggtg aataccgtgg cgtacaggcg cagctcggct
     2161 tcggcacggc cgagcgcagc ggcgtgttcc tgcaggtcgg catgcgcctt gctgacagtg
     2221 agcgagaccg cgcgcagtcg cagcgcggtg agcagcaggt aggcgccgag cgcgagcgcg
     2281 gcgagcacca gcagcacgcg gtggcggtgc atgcgggttt gcgaggcgac gtagccgtcg
     2341 gtgtagctct ccaccaggcg ctcgccctcg cgtgcggtct gcagcgcgag gagctggcgc
     2401 aggaggcggt cggcctcggc ttcggccgcg ccgccgttct gcggcggcgc gtcgaggagc
     2461 acgcgcagcg cgctgatcac ggtgcgcttg tcggtctgca gggcggtgta gtgatcgagc
     2521 tggcggcgca cggccgccat ctcgctgccg gtgaggaagt cgggaacgcg gccgaggtct
     2581 tcgaggagtt cacccatccg cgccaggtgg gcgtcggccc cgtgccagtc ctcggcgagc
     2641 ccgagccggc gggcgagcgc ggcggcgccg agctcggcat cgacctggcg gatctcgcgc
     2701 aggtcgcggg cgtagcgcag gtgctcgcgt gccgagctgg tgccggcctc gaccacgaac
     2761 caccccagcc cggcgaggcc gatcagcgcg agcacgagca ggcggatgct gctcgaggtg
     2821 tgcagcagca gcgcgaaggc ggcgcgcgtc acttcctccg ctccggagcg aggggccggg
     2881 gagcgggggg caaggttgag ccggggagaa gggaggtggg caaggcgggc acgggcggat
     2941 cctatctgcg cgtcttgtcc tggaattgat ctagatcaaa ggttggatga taggacatcc
     3001 gggtcgggca tgccgatcca ctatgccggg atggccccgt ccacgacagc ttcacttctc
     3061 cacgaacgcg cgctcgatca cgtagtcgcc cgccacgccg atgcgcggcg acatcttgaa
     3121 gccgcgcgcg tcgagcaggt cgcagcagtc cttgttcatc gcctggctgc cgcagatcat
     3181 tacgcggtcg tgcccgggat cgagctccgg caggccaatg tcggcgaaga gccgaccgga
     3241 gtcgatcaca tgagtgatgc gtccggtgtt gcggaagggc tcgcgcgtca ccgtcggata
     3301 gtagatcagc tgctcgcgca cctgctcgcc gaagtattca tgctgcggca gctcgcgggt
     3361 gatgaagtcg gtgtaagcga gttcggagac gaaacgcaca ccatgcacca gcacgacctt
     3421 ctcgaagcgc tcgtaggtct ccgggtcctg gaccacgctc aggaagggcg ccaggccggt
     3481 gccggtggcg agcaggtaga ggtgtttgcc cgggttgagg tcgtgcagca ccagggtgcc
     3541 ggtgggcttc ttgctcacca cgatcgggtc gcccgggcgc aggtgttgca ggcgcgaggt
     3601 cagcgggccg tccggcacct tgatgctgaa gaactcgagg tgctcctcgt agttggggct
     3661 ggcgatgctg taggcgcgcg tcagcggctt gccgccgacg tccaggccga tcatcacgaa
     3721 ctggccgttc tcgaagcgca gcccggggtc gcgggtggtg cggaagctga agagggattc
     3781 gttccagtga tggacgctga ggacgcgttc ggtggcgagg ctgctcatgg tttcgggtct
     3841 ctgcaggctg cgggatggag gagtgagcgg attctaggta cttctcctta cctgtatggc
     3901 ggataatatt catatccttc atcgacttta tcgatatgaa gtttaccctc cgccagatcc
     3961 aggtcttcgt cgcggtggcg cgcgccgagc acgtctcgcg cgctgccgag ggcctcgcgt
     4021 tgtcgcagtc ggcggcgagt gcggcgctcg ccgagctgga gagtcagtgc gcgcagccgc
     4081 tcttcgaccg ccacggcaag cgcctgcgcc tgaacgagca gggcagtctg ctgctgccgc
     4141 atgcggtgga gctgctcgac cgtgccgccg agtttgaggg ccgcatgcgc ggcgagcagg
     4201 gcctcggccc gctgcgcgtg ggcgcgacgc tgaccatcgg caactacctg ctgccgctga
     4261 tcgtgtcgga tttcctccag gcccaccccg agagccgggt gcatctgcag gtgcgcaaca
     4321 ccgcgaccat cgccgagcgc ctgctgcacc acgagatcga cctgggtctg gtcgagggcc
     4381 aggtggtgga tgccgagctg cacgtcgagc cctgggtcga ggacgagctc gtggtgttct
     4441 gcgcgccggg gcatgcgctc gccgcacgcg gtcgcgcgag cgtggccgag ctcgccggcc
     4501 agccgtggat cctgcgcgag cgtggttcgg gcacgcgcga gaccttcgat cgcgcgttgc
     4561 ggcatcaccc gggcggggtc gtgccgcgac tggagctcga gcataccgag gccatcaagc
     4621 gcgcggtgga gagcggcctg ggcttgggtt gcctgtcgcg gctggcgctg cgcgatgcgt
     4681 tccggcgcgg cagcctggtg gcggtggagg tgcccgagct cgacctgcgc cggcgcttca
     4741 gcttcgtctg gcatcgcgga aaatatcacg gtgccgcgct gcgccgcttc gtcgaggatt
     4801 gccgcgcctt caccgccggc gcccgccgca gcgacgagat cccgctgccg ccggtggctt
     4861 gaattcgtcg cggtgtttcg ttcggcgctg cgatcgcgac ttgcgtcgct cccacaat
//
LOCUS       DAOQNN010000244         4910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10442_length_4910_cov_9.123790, whole genome shotgun sequence.
ACCESSION   DAOQNN010000244 DAOQNN010000000
VERSION     DAOQNN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10442_length_4910_cov_9.123790"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PKV97_12060"
     CDS             <1..489
                     /locus_tag="PKV97_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNB06659.1"
                     /translation="LWRFREGYDPYVKAGEGVKFYGHKDNKAVVFALPYQDPPEMPDA
                     EFDMWLCTGRVLEHWHTGSMTRRVPELHRSVPEAQIFMHPEDAQKRGLQRGMAVKVVS
                     RRGEIVARLETRGRNKPPVGLIFVPFFDEGRLVNKLTLDATCPISKETDYKKCAVKVL
                     KV"
     gene            552..1451
                     /gene="napG"
                     /locus_tag="PKV97_12065"
     CDS             552..1451
                     /gene="napG"
                     /locus_tag="PKV97_12065"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HNB06660.1"
                     /translation="MLMNDKVQSTGRVEPVAPASRRRFLKDAAGVAGGAGLLALGAGM
                     YAKQASALPATAVRPPGALPEDDFLAACVRCGLCVRDCPYKTLKLAEFGDGVATGTPY
                     FEARDIPCEMCEDIPCVVACPTGALDRALVDITKAKMGLAVLIDQENCLNFLGLRCDV
                     CYRVCPVIDQAITLERMHNPRSDRHAMLLPTVHSEHCTGCGKCEKACVLPGEAAIKVL
                     PIKLAQGSKAEHYLRGWEEKEAAGKSLIGDQVELPVRGLEGKAYGDTRVEPGQGPVQA
                     PAQVPDYVPAKPAGGGLDSGWKP"
     gene            1448..2383
                     /gene="napH"
                     /locus_tag="PKV97_12070"
     CDS             1448..2383
                     /gene="napH"
                     /locus_tag="PKV97_12070"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HNB06661.1"
                     /translation="MSARLGAGGVPERKVPPTSVRGAAMRGVVARPGREAVEAKGWLR
                     AHKWLLLRRASQLGILALFLVGPWLGWWIVKGNLSSSLTLGVLPLTDPFLVVQQFAAG
                     HWSYREALLGAGIVLAFYLLFGGRLFCSWVCPVNLVTDAAAWLRRRLGLKGGKVPSGS
                     TRYWLLGFVLVIAAATGSLAWEWVNPVSMFHRGLIFGFGLAWGIVGGIFFYDLLIAPR
                     GWCGHLCPQGAFYALLGRAAVVKVSAAKRSACNDCMDCFAVCPEPQVIRPALKGVGQD
                     HPLILDADCTTCGRCVDVCGKDVFRITTRFNRSES"
     assembly_gap    2388..2487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2488..2928
                     /locus_tag="PKV97_12075"
     CDS             <2488..2928
                     /locus_tag="PKV97_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HNB06662.1"
                     /translation="TRNLVLALVAAVGFGAMVPPAAMAQAVKSIRGADVAEQELQVGN
                     YKPIPDQAPIERDYVQQPPLIPHKVEGYEVTINFNKCMDCHAWSRYKESGATKVSLTH
                     FKDRDGGELSNISPRRYFCMQCHVPQTDAKPLVGNKFQRAEGLR"
     gene            2952..3572
                     /locus_tag="PKV97_12080"
     CDS             2952..3572
                     /locus_tag="PKV97_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNB06663.1"
                     /translation="MTDNNKSLMQRIRGAGWGAIVLLVAAGVVFWGGFNWALEMTNTE
                     KFCISCHEMEQNVFKEYRNTIHYQNRTGVRATCPDCHVPKEWGPKMIRKIKASRELYG
                     KVMGTIDTPEKFAAERLRLAQNEWNRMKANNSQECRNCHNYEYFDYSVQGRRSNQMHQ
                     AGFAEGKTCIDCHKGIAHSLPPVDQHIGAPREGVAPEVMHPPAKKD"
     gene            3691..4317
                     /gene="ccmA"
                     /locus_tag="PKV97_12085"
     CDS             3691..4317
                     /gene="ccmA"
                     /locus_tag="PKV97_12085"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HNB06664.1"
                     /translation="MLEVESLACLKGDRLLFRDLAFRLQAGGLLRVAGPNGVGKTSLL
                     RLVTGLALPEAGEIRWRGESIRRAREAFHGDLLYLGHAAALNDLLSPLENLRFACAAA
                     GDAVDAEDCVQALQRIGLADQLDLPARVLSQGQRRRVGLARLFLSNARPLWVLDEPFT
                     ALDVRAVADLAATLSQHCEAGGMVMLTTHQDAPFARPPEVLDVGVFAC"
     gene            4311..>4910
                     /gene="ccmB"
                     /locus_tag="PKV97_12090"
     CDS             4311..>4910
                     /gene="ccmB"
                     /locus_tag="PKV97_12090"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNB06665.1"
                     /translation="MLSTFLAVLRRDLLLAWRGRADVLVTLAFFIIVVCLFPFGVGAE
                     PNQLRAIAPGVLWVAALLACLLSLHRLFAQDYSDGTLEQLLLSSEPAALWVMAKVLAF
                     WLSTGLPVVAVAPAMGLLLDLEQGGLPVLILSLLLGTPILALLGAVGAALTLGLRGGG
                     MLLALLVLPLFVPVLIFGAGAVEAELSGSGAAAHLLLLGG"
BASE COUNT          696 a         1666 c         1667 g          781 t
ORIGIN      
        1 ctgtggcgct tccgcgaggg ctatgacccg tacgtgaagg cgggcgaggg cgtgaagttc
       61 tacggccaca aggacaacaa ggcggtcgtg ttcgcgctgc cctaccagga tccgcccgag
      121 atgcccgacg ccgagttcga catgtggctg tgtaccggcc gcgtgctgga gcactggcac
      181 accggctcga tgacccggcg cgtgcccgag ctgcaccgct cggtgcccga ggcacagatc
      241 ttcatgcacc ccgaggacgc gcagaagcgc ggcctgcagc gcggaatggc ggtcaaggtg
      301 gtgtcgcggc gcggcgagat cgtcgcccgc ctggagaccc gcggccgcaa caagccgccg
      361 gtcgggctca tcttcgtgcc cttcttcgac gaaggccggc tggtgaacaa gctcacgctc
      421 gacgccacct gcccgatctc gaaggagacc gactacaaga aatgcgcggt gaaggtcctt
      481 aaggtctgat gagaggaggc gcgagtccgg cccccgccga ccggggggac gcgcggaggg
      541 cggggctcgc gatgctgatg aacgacaagg tgcaatcgac cggacgcgtt gaacccgtcg
      601 ctccggcttc gcgccgccgc ttcctcaagg atgcggcggg cgtcgccggc ggtgcgggtc
      661 tgctcgcgct cggtgccggg atgtacgcga agcaggcgtc ggcgttgccg gcgacggccg
      721 tccgtccgcc cggcgctctc cccgaagacg attttctcgc ggcctgcgtg cgctgcggcc
      781 tgtgcgtgcg cgactgtccc tacaagaccc tgaagctggc cgagttcggc gacggggtgg
      841 cgacgggcac gccgtacttc gaggcgcgcg acattccctg cgagatgtgc gaggacatcc
      901 cctgcgtggt ggcctgtccg accggcgcgc tcgaccgtgc gctggtcgat atcaccaagg
      961 cgaagatggg gctggcggtc ctgatcgacc aggagaactg cctcaacttc ctcggcctgc
     1021 gctgtgacgt gtgctaccgg gtgtgcccgg tcatcgacca ggcgatcacc ctcgagcgca
     1081 tgcacaaccc gcgctccgac cgtcacgcga tgctgctgcc gaccgtgcat tccgagcact
     1141 gcaccggctg cggcaagtgc gagaaggcct gcgtgctgcc cggcgaggcg gcgatcaagg
     1201 tgttgccgat caagctggcg caaggctcga aggccgaaca ctacctgcgt ggctgggaag
     1261 agaaggaggc cgccggcaag tcgctcatcg gcgaccaggt cgagctgccg gtgcgcggcc
     1321 tcgaaggcaa ggcctacggc gacactcggg tcgaacccgg gcaagggccg gtccaggcgc
     1381 ccgcgcaggt gccggattac gttcccgcga agccggcggg cggtggtctc gactcggggt
     1441 ggaagccatg agcgcgcgac tcggcgccgg cggcgtgccg gaacgcaagg tcccgcccac
     1501 cagcgtgcgc ggcgccgcga tgcgcggcgt ggtcgcacgt cccgggcgcg aggcggtgga
     1561 ggcgaagggc tggctgcgcg cgcacaagtg gctgctgctg cggcgcgcgt cgcagctcgg
     1621 catcctcgca ctcttcctcg tggggccatg gctggggtgg tggatcgtca agggcaacct
     1681 gtcgtccagc ctcaccctcg gcgtgctgcc gctcaccgac cccttcctgg tcgtgcagca
     1741 gttcgccgcc gggcactggt cctaccggga ggcgctgctc ggcgccggca tcgtgctcgc
     1801 cttctatctg ctcttcggtg ggcggctgtt ctgctcgtgg gtgtgtccgg tcaacctcgt
     1861 caccgacgcc gccgcctggt tgcgccggcg cctgggcctc aagggtggca aggtgccgtc
     1921 gggcagcacg cgctactggc tgctcggctt cgtgctggtg atcgcggcgg cgaccggctc
     1981 gctggcgtgg gagtgggtta atccggtgtc gatgttccac cgcggcctga tcttcggttt
     2041 cggactggcc tggggcatcg tcggcggcat cttcttctac gacctgctca tcgccccgcg
     2101 cggctggtgt ggccacctgt gcccgcaagg cgccttctac gccctgctcg gacgcgccgc
     2161 cgtcgtcaag gtctcggccg ccaagcgcag cgcgtgcaac gactgcatgg actgcttcgc
     2221 cgtctgcccc gagccccagg tcatccggcc cgcgctcaag ggcgtaggcc aggaccaccc
     2281 cctcattctg gatgccgact gcaccacctg cggacgctgc gtggatgtgt gcggcaagga
     2341 cgttttccgc attacgaccc gattcaacag gagcgagtca tgaaaaannn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnacc cgaaatctgg tgctcgcgct cgtcgccgct
     2521 gtcggcttcg gtgccatggt gccgcccgcg gcgatggcgc aggcggtgaa gagcatccgc
     2581 ggcgccgacg tggccgagca ggagctgcag gtcggcaact acaagccgat tcccgaccag
     2641 gcgccgatcg agcgcgacta cgtgcagcaa ccgccgctga tcccgcacaa ggtggagggc
     2701 tacgaggtca cgatcaactt caacaagtgc atggactgcc acgcctggag ccgctacaag
     2761 gagtcgggtg ccaccaaggt cagcctgacc cacttcaagg atcgtgacgg cggcgaactg
     2821 tcgaacatct cgccgcgccg ctatttctgc atgcagtgcc atgtgcccca gaccgacgcc
     2881 aagccgctgg tcggcaacaa gttccagcgc gcggaaggcc tgcgctgaag aatcgacaca
     2941 cggggatcga catgaccgac aacaacaaga gcctgatgca gcgcatccgc ggtgcgggct
     3001 ggggcgccat cgtcctgctc gtcgccgcgg gcgtggtgtt ctgggggggc ttcaactggg
     3061 ccctggagat gaccaacacg gagaagttct gcatttcctg tcatgagatg gagcagaacg
     3121 tcttcaagga gtatcgcaac accatccact accagaaccg caccggggtg cgcgcgacct
     3181 gcccggactg ccacgtgccc aaggagtggg gcccgaagat gatccgcaag atcaaggcct
     3241 cgcgcgagct ctacggcaag gtgatgggca ccatcgacac gccggagaag ttcgccgccg
     3301 agcgcctgcg cctggcgcag aacgagtgga accgcatgaa ggcgaacaac tcgcaggagt
     3361 gccgcaactg ccacaactac gagtatttcg actactcggt gcagggccgt cgctccaacc
     3421 agatgcacca ggccggcttc gccgagggca agacctgcat cgactgccac aagggcatcg
     3481 cgcactcgct gccgccggtc gaccagcaca tcggcgcgcc gcgcgaaggc gtcgctcccg
     3541 aggtgatgca cccgcccgcg aagaaggact gagctgcgcc gcagcccccg ccccgcgctg
     3601 tgccgcatcg agcggtgcgc cgcggggcgc gtctttccgg tgtgtgccgg gatggcgcat
     3661 cccgggccgc ctgtgttcca atccgtcccc atgctcgaag tcgaaagtct cgcctgcctg
     3721 aaaggcgatc gcctgctgtt ccgcgacctc gccttccgcc tccaggcggg tggcctgctg
     3781 cgcgtcgccg ggcccaacgg ggtcggcaag accagcctgc tgcgcctggt caccgggctg
     3841 gcgctgcccg aggccggcga gatccgctgg cgcggcgagt cgatccggcg cgcgcgcgag
     3901 gccttccacg gcgacctgct ctacctcggc cacgccgccg cgctcaatga cctcctcagc
     3961 ccgctggaga acctgcgttt cgcctgcgct gccgccggcg acgcggtcga cgcggaggac
     4021 tgcgtgcagg ccctgcagcg catcggcctc gccgaccagc tcgacctgcc ggcgcgcgtg
     4081 ctgtcgcagg gccagcgccg ccgcgtcggc ctggcgcggc tgttcctgtc gaacgcgcgc
     4141 ccgctgtggg tgctcgacga gcccttcacc gcgctcgacg tgcgtgcggt cgccgatctc
     4201 gccgccacgc tctcgcagca ctgcgaagcg ggcggcatgg tgatgctcac cacccaccag
     4261 gacgcgccct tcgcgcgccc gcccgaggtg ctcgacgtcg gagtcttcgc atgctgagca
     4321 ccttcctcgc cgtgctgcgg cgcgacctgc tgctggcctg gcgcgggcgc gccgacgtgc
     4381 tcgtcacgct cgccttcttc atcatcgtgg tgtgcctgtt ccccttcggc gtcggcgccg
     4441 agcccaacca gctgcgcgcg atcgcgcccg gcgtgctgtg ggtcgcggcg ctgctcgcct
     4501 gcctgctttc gctgcatcgc ctgttcgcgc aggactacag cgacggaact ctcgagcagc
     4561 ttctcctgtc tagcgagccg gctgcgctgt gggtgatggc caaggtgctg gcgttctggc
     4621 tcagcaccgg cctgccggtg gtggcggtgg cgccggccat ggggctgctg cttgacctcg
     4681 aacaaggcgg gctgcccgtg ctgatactaa gtttgctgct cggcacgccc atcctcgcgc
     4741 tgctcggtgc ggtcggcgcg gcgctgaccc tcgggctgcg cggcggtggc atgctgctgg
     4801 ccctgctggt gctgccgctg ttcgtcccgg tgctgatctt cggtgccggc gcggtcgagg
     4861 ccgagctgtc gggtagcggc gccgccgccc atctgctgct gctcggcggt
//
LOCUS       DAOQNN010000245         4870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10583_length_4870_cov_8.280166, whole genome shotgun sequence.
ACCESSION   DAOQNN010000245 DAOQNN010000000
VERSION     DAOQNN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10583_length_4870_cov_8.280166"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PKV97_12095"
     CDS             complement(<1..487)
                     /locus_tag="PKV97_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06666.1"
                     /translation="MSEVRTEQESPQRHKAGAAQVEKPQVSQAPQAPASKDSQLLPLA
                     ALAAPGLAGALLLGASSPALAAAKTDPNEKTTVTQIQWRISYSVEIPGNCMVAGFSQW
                     QKSKFRGWDVYKLSFRWNSPGTPESSNSLLSPPPKYDDELKYGGNVVSPPGNSHWVLI
                     GD"
     gene            696..2201
                     /locus_tag="PKV97_12100"
     CDS             696..2201
                     /locus_tag="PKV97_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB06667.1"
                     /translation="MLIRRQQWAIAIASLITVVVATGLWIANNLALRAELERGRMSAQ
                     IGPVGAAQMRSRIFEFLAEPSPAARARWYESHQALGALLAPALMDGTPGEPIIAGLRA
                     RHAELPALFEALAADRLREAAASTPTPELLDAQRMASVHFMEATQQVASDTLRLLDLG
                     QARLERLHADQERLIAGLILALGLVIGLELFLVHRRILDPLQELHKGARAIEKGHYEH
                     RLGMSGKDEIAQVGAQFDHMADALGETLQALHQQRSQLELANRELESFSYSVSHDLRA
                     PLRGIGGWAQALEEDHGAALPVEAQEYLARIRGECARMNLLIEDLLQLARVTRSEPSF
                     EWLELDLLAREVAEHVRESWPGRDIEFVIAPGLTVWGDRRLLGIALANLFDNACKFTA
                     RVPRARVELGCREIVDPLGTRVLRQYFVRDNGAGFDMKHAARLFVPFQRMHSQREFPG
                     TGIGLAIVQRIIQRHAGVLEAEAGPGEGACFRFTLPLLPGPPAPVPNATPP"
     gene            2212..3759
                     /locus_tag="PKV97_12105"
     CDS             2212..3759
                     /locus_tag="PKV97_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB06668.1"
                     /translation="MTPPPNSAPAPSTPSPADSRSATAPRELAVLMVEDSESDALLVL
                     RALRRAGFALRHRRVEDGAGMRAALAGERWDVVLSDFNLPGFDARSALAELHASGLDL
                     PFIVVSGTIGDEIAIELMRRGAHDYLMKENLARLAPAIERELGEARVRAERRRALAAL
                     HDSEAVLREAQRIGGVGQWRRDLPGGAPWCSDTLFALFGMAPVAGEDALDALLTRVHP
                     DDLGRLLQAHRELAAGSEHVELEYRIVHADGSLRYVLECAARSRGVEAATPRLIGTVH
                     DVTAQRAAAEALRTANARLRQMSLRVLDAQEAERRAVAYELHDQIGQALTAVKLDLQA
                     MTPHLDAGPAVARLAAAIHTTEEALAQVRGLSLNLRPPQLDYMGLEASLRWHAERECA
                     RAGLALDFAGSVGEFKAEERCAIVCFRLLQEALTNVVRHAAAKRVRIALSAADGRLDL
                     EIADDGRGFDLARARRRMLEGASVGLLGMEERAALIGGEVHIDAAPGAGTRIRVSLPC
                     TAHAAAA"
     gene            3769..4449
                     /locus_tag="PKV97_12110"
     CDS             3769..4449
                     /locus_tag="PKV97_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB06669.1"
                     /translation="MEGMRVLLVDDHSLVRAGIRALLGSIAGVAGIEEASSGRQAVEL
                     AATMQPDLVLMDIAMDDMDGLAATRTIAAAHPHIKVVILSMHDTGDFFEQALRCGARG
                     YLLKDAAVAELEMAIAAVMRGDVYLSPAVSRHLVGNMLARPPEDERAASVLTPRQTEI
                     LELIGSGLGTKEIARRLDLSVKTVEAHRAQIMERLGVRDIANLLLEAARRGLITLGKR
                     STPLDAPR"
     gene            4549..>4870
                     /locus_tag="PKV97_12115"
     CDS             4549..>4870
                     /locus_tag="PKV97_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB06670.1"
                     /translation="MGETVNLLIVEDNESDAALVVRQLERAGYTVATARRVDRAAELG
                     AALEACAWSAVLCDYALPGFSPEEALAQVQASGLDLPFVVVSASLADEEAGRLMALGA
                     HDYVH"
BASE COUNT          690 a         1766 c         1730 g          684 t
ORIGIN      
        1 cgtcaccgat cagcacccag tggctgttgc cgggaggcga gacgacattg ccgccgtact
       61 tgagctcgtc gtcgtacttc ggcggcggag agaggagcga gttgctcgac tcaggagtgc
      121 cgggagagtt ccaccggaag gacagcttgt acacgtccca gcccctgaac ttggacttct
      181 gccactgcga gaacccggcg accatgcagt taccggggat ctcgacgctg taggagatcc
      241 gccactggat ctgagtgacc gtggtcttct cgttggggtc ggtcttggcc gccgcgaggg
      301 cgggcgagga ggcgccgagc agcagcgccc ccgccaaccc gggcgcggcg agcgcagcca
      361 ggggcaggag ctgcgaatcc ttcgaggcgg gagcctgggg ggcttgggac acttgaggct
      421 tctccacttg tgccgcaccc gccttgtggc gctgcggcga ttcctgttcc gtgcggactt
      481 cgctcacgga gcgctccgcg cgaagatgga acccatccgg ggactgccgt taaactccgc
      541 atccgcccgg gggtttgccg gcggcatgca tttccgtcgc ctgcatgttc ccgctccggg
      601 gcgtctaggg acaatcggta aaattccgtg catgccccta ggggattgga ctagtcggaa
      661 agcaggagca ggctcatcga gacggaggga ttcgcgtgct gatccgcagg cagcaatggg
      721 cgatcgcgat cgcgtcgctg atcaccgtcg tggtcgccac ggggctgtgg atcgcgaaca
      781 acctggcctt gcgcgccgaa ctggagcgcg gccgcatgtc ggcgcagatc gggccggtgg
      841 gcgccgcgca gatgcgctcg cgcatcttcg agttcctcgc cgagccctcg ccggccgcgc
      901 gcgcgcgctg gtacgagagc caccaggcgc tcggcgcgct gctcgcgccc gcgctgatgg
      961 acggcacccc cggcgagccg atcatcgcgg gcctgcgcgc acgccacgcg gaactgccgg
     1021 cgctgttcga ggcactcgcc gccgatcgcc tgcgcgaggc cgcagccagc acgccgacgc
     1081 ccgagttgct cgacgcgcag cgcatggcct cggtgcactt catggaggcg acgcagcagg
     1141 tggccagcga caccctgcgc ctgctcgatc tcggccaggc ccggctggaa cggctgcatg
     1201 ccgatcagga gcgcctgatc gccggcctca tcctcgcact gggcctggtg atcggcctgg
     1261 agctcttcct ggtgcatcgc cgcatcctcg atccgctgca ggaactgcac aagggcgcgc
     1321 gggcgatcga gaagggtcac tacgagcacc gcctcggcat gtccggcaag gacgagatcg
     1381 cccaggtcgg tgcgcagttc gaccatatgg ccgacgcgct cggcgagacc ctgcaggccc
     1441 tgcaccagca gcgcagccag ctcgaactcg ccaaccgcga gctggagagc ttcagctact
     1501 cggtgtcgca cgacctgcgc gcaccgctgc gcggcatcgg cggctgggcg caggcgctcg
     1561 aggaggatca cggcgccgcc ctgcccgtgg aagcgcagga atacctcgcg cgcatccgcg
     1621 gcgaatgcgc gcgcatgaac ctgctcatcg aggacctgct gcagctggcc cgcgtcaccc
     1681 gcagcgagcc gtccttcgag tggctggagc tcgacctgct cgcccgcgag gtggccgagc
     1741 acgtgcgcga gagctggccg gggcgcgaca tcgagttcgt gatcgccccc gggctgacgg
     1801 tctggggcga ccgccgcctg ctcgggatcg cgctcgccaa cctgttcgac aacgcctgca
     1861 agttcaccgc gcgcgtgccg cgggcgcgcg tcgaactcgg ctgccgggaa atcgtcgatc
     1921 ccctcggcac gcgcgtgctg cggcagtatt tcgtgcgcga caacggcgcg ggcttcgaca
     1981 tgaagcacgc cgcccggctg ttcgtgccct tccagcgcat gcactcgcag cgcgagtttc
     2041 ccggcaccgg catcgggctg gcgatcgtgc agcgtatcat ccagcgtcac gccggcgtgc
     2101 tggaggccga ggccgggccc ggcgagggcg cgtgcttccg cttcaccctg ccgctgctgc
     2161 ccggaccgcc tgcccccgta ccgaacgcaa cgcccccatg aggcgagcgc cttgacccct
     2221 cccccgaact ccgcccctgc cccctccacg ccctcgcctg cggacagccg cagcgcaacc
     2281 gcgccacgag agctcgcggt gctgatggtg gaagactccg agagcgatgc gctgctggtc
     2341 ctgcgtgcgc tgcggcgtgc cggcttcgcg ctgcgccatc gccgcgtcga ggacggtgcc
     2401 ggaatgcgcg cggcgctcgc cggcgagcgc tgggacgtgg tgctctccga cttcaacctg
     2461 cccggcttcg acgcgcgcag tgcgcttgcc gagctgcacg cgagcgggct agacctgccc
     2521 ttcatcgtgg tgtcgggcac catcggcgac gagattgcga tcgagctgat gcgccgcggc
     2581 gcccacgact acctgatgaa agagaacctc gcccgcctcg cccccgcgat cgagcgcgag
     2641 ctcggcgagg cgcgcgtccg cgccgagcgc cgacgcgcgc tcgccgccct gcacgacagc
     2701 gaggcggtgc tgcgcgaggc gcagcgcatc ggtggggtcg ggcagtggcg cagggacctg
     2761 ccgggcggcg cgccatggtg ctcggacacc ttgttcgcgc tcttcggcat ggccccggtg
     2821 gcgggcgagg atgcgctcga cgccctgctc acccgcgtgc acccggacga cctcggccgc
     2881 ctgctgcagg cgcatcgcga gctcgcggcc ggcagcgagc acgtcgagct ggagtaccgc
     2941 atcgtccatg ccgacggcag cctgcgctac gtgctcgagt gcgccgcccg cagccgcggc
     3001 gtcgaggctg cgacgccgcg gctgatcggc acggtgcacg atgtcaccgc gcagcgcgcc
     3061 gcggccgagg ccttgcgcac cgccaacgcg cgcctgcgcc agatgtcgct gcgcgtgctc
     3121 gacgcccagg aggccgagcg ccgtgcggtg gcctacgagc tgcacgacca gatcggccag
     3181 gcgctgaccg cggtcaagct cgacctgcag gcgatgaccc cgcatctgga cgccggcccg
     3241 gcggtggcgc gcctggccgc ggccatccac accaccgagg aggcgctcgc gcaggtgcgc
     3301 ggcctgtccc tgaacctgcg cccgccgcag ctcgactaca tgggcctgga ggcctcgctg
     3361 cgctggcacg ccgagcgcga atgcgcgcgc gccgggctgg cgctcgactt cgccggcagc
     3421 gtgggcgagt tcaaggcgga agagcgctgc gcgatcgtgt gcttccgcct gctgcaggag
     3481 gcgctcacca acgtcgtccg ccatgcggcg gcgaagcggg tgcgcatcgc cctgtcggcc
     3541 gcggacggcc gcctggacct ggagatcgcc gacgacggcc gcggcttcga cctcgcgcgc
     3601 gcgcgccggc gcatgctgga gggcgccagc gtgggcctgc tcggcatgga ggagcgcgcc
     3661 gcgctcatcg gcggcgaggt ccacatcgac gcggcgcccg gcgcgggcac ccgcatccgg
     3721 gtcagcctgc cctgtaccgc acacgccgcc gcggcgtagg attcggccat ggaaggaatg
     3781 cgagttctgc tggtggacga tcacagcctg gtgcgcgcgg gcatccgcgc gctgctcggc
     3841 tccatcgcgg gcgtggccgg catcgaggag gcgagcagcg gccgtcaggc ggtcgagctg
     3901 gcggcgacga tgcagcccga cctggtgctg atggacatcg cgatggacga catggacggc
     3961 ctcgccgcca cacgcacgat cgccgcggcg catccgcaca tcaaggtggt gatcctgtcg
     4021 atgcacgaca ccggcgactt cttcgagcag gcgctgcgct gcggcgcgcg cggctacctg
     4081 ctcaaggacg ccgcggtggc cgagctcgag atggcgatcg cggcggtgat gcgcggcgac
     4141 gtctatctga gcccggcggt gtcgcgccac ctggtgggca acatgctcgc ccgcccgccc
     4201 gaggacgaac gcgcggcgag cgtgctgacc ccgcgccaga ccgagatcct cgagctcatc
     4261 ggcagcggcc tcggcaccaa ggagatcgcc cgccggctcg acctcagcgt caagaccgtg
     4321 gaggcgcacc gcgcgcagat catggagcgc ctgggcgtgc gcgacatcgc caacctgctg
     4381 ctcgaggccg cgcgccgcgg cctcatcacg ctcggcaagc gctccacacc cctagacgcg
     4441 cctcggtaat ccaagggatt gcccccaccg cccgccggct tatcatcgcc gcatggaccg
     4501 gagtgccgta gcgcgcttgc ggccgcaccc cgcaatggaa gggcaagcat gggcgagacc
     4561 gtcaacctgc tgatcgtcga ggacaacgaa tccgatgccg cgctggtcgt gcgccagctc
     4621 gagcgcgccg gctacacggt ggcgacggcg cgccgcgtcg atcgcgccgc ggaactcggc
     4681 gccgcgctcg aggcctgcgc gtggtccgcg gtgctgtgcg actacgccct gcccgggttc
     4741 agccccgagg aggcgctcgc ccaggtgcag gcgagcggcc tcgacctgcc cttcgtggtc
     4801 gtctcggcct cgctcgccga cgaggaggcc ggccgcctga tggcgctcgg cgcacacgac
     4861 tacgtgcaca
//
LOCUS       DAOQNN010000246         4860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10610_length_4860_cov_7.749636, whole genome shotgun sequence.
ACCESSION   DAOQNN010000246 DAOQNN010000000
VERSION     DAOQNN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10610_length_4860_cov_7.749636"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..939)
                     /gene="rsmH"
                     /locus_tag="PKV97_12120"
     CDS             complement(4..939)
                     /gene="rsmH"
                     /locus_tag="PKV97_12120"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNB06671.1"
                     /translation="MNLAHAHVSVLLSEAVDALAIRADGFYVDGTFGRGGHSRAVLQR
                     LGPQGRLIAFDRDPLAIAAGQAVGDARLTLVHSAFSALDDELARLGVQAVDGVLLDLG
                     VSSPQLDDAARGMSFRFDAPLDMRMDTSRGQTVAEWLAEASVGQITEVIREYGEERFA
                     HAIAKAIATARAGGAVATTGQLAALVEKAVRTREPGQHPATRTFQALRIFINQELEEL
                     SRVLPVCVSRLRPGGRLAVISFHSLEDRIVKRFMRDESRPPVLPRRLPVRAADLPAPR
                     LQLVGKAMRPSEAEVAANPRARSAVLRVAERTEAP"
     gene            complement(936..1379)
                     /gene="mraZ"
                     /locus_tag="PKV97_12125"
     CDS             complement(936..1379)
                     /gene="mraZ"
                     /locus_tag="PKV97_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNB06672.1"
                     /translation="MFQGAVALNLDAKGRLAIPARHRDALAVDNGQVVLTAHPHGCCL
                     VYPVPAWNPIRDHVLRAPSLDPQAAMLKRLLVGFAQEETLDAAGRVLVAPSLRKFAAL
                     EKQVWLVGQGAHFELWSDERWEKQQQAMLELVNTGLPPGFESLAL"
     gene            complement(1740..2213)
                     /gene="rnpB"
                     /locus_tag="PKV97_12130"
     ncRNA           complement(1740..2213)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKV97_12130"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2254..2757)
                     /locus_tag="PKV97_12135"
     CDS             complement(2254..2757)
                     /locus_tag="PKV97_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06673.1"
                     /translation="MGVRMHETGARDAGAPMSIRRGVRGGAGRALRRGLAAVGLLAVL
                     AGCENSATAYMIDGNQHALILVREQKFFWDDELRQAMVVSRLPACQKRIRIHPGSATL
                     VEMKIYAAGDGLWALHQGARWYLAGTEECRVQDWDNPGDRPPGALVGSFVLKDGAPAF
                     VPAPAAK"
     gene            complement(2759..3799)
                     /gene="pyrC"
                     /locus_tag="PKV97_12140"
     CDS             complement(2759..3799)
                     /gene="pyrC"
                     /locus_tag="PKV97_12140"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNB06674.1"
                     /translation="MQSITLIRPDDWHLHVRDDAALDAVVPHTAARFGRALIMPNLKP
                     PVTTTAQALAYRERILAAARGSRFEPLMSLYLTDNLSPDEIDRARASGHVVACKLYPA
                     GATTNSDAGVTAIDKIYAVLERMEKLGMVLCVHGEATGAEVDVFDRERVFVERTLSPL
                     VRRFPGLKVVFEHITTAEAAQFVRAAGSHVAATVTAHHLLLNRNAIFAGGIRPHHYCL
                     PVLKRESHREALIAAVTSGNARFFLGTDSAPHARSTKENACGCAGCYTAHAGIELYAE
                     VFDAAGALDRLEAFASLNGPAFYGLAPSSDTITLRREPWSVPASYPYLGEDPLVPLRA
                     GEQVGWKLVEGA"
     gene            complement(3874..4761)
                     /gene="rsmI"
                     /locus_tag="PKV97_12145"
     CDS             complement(3874..4761)
                     /gene="rsmI"
                     /locus_tag="PKV97_12145"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNB06675.1"
                     /translation="MTVSAPLPDRPLSSTPSLYVVATPLGNLHDITLRALAVLAAVDV
                     VAAEDTRHSQRLLDAHGIRARMLAAHQHNEHEAAGVIVAELAAGRQVALITDAGTPAV
                     SDPGARLVARVRAAGHPVVPLPGPCAAVAALSVSGFAEGGFRFVGFLPAKAGARAAAL
                     RELADERAAMVFHEAPHRIVECVADMEQVLGGARELLIAREMTKLHEQIVRMPLAQTA
                     AWFAADTNRLRGEFVLVVEGAPAGEVVDARARRVLDLLLAELPVKGAARLAAEITGAP
                     RKALYALALERKGEDSPQA"
BASE COUNT          753 a         1707 c         1701 g          699 t
ORIGIN      
        1 ccatcagggc gcctccgtgc gctcggccac gcgcagcacc gcgctgcgcg cgcgcgggtt
       61 ggccgcgacc tcggcctcgc tcgggcgcat tgccttgccg acgagttgca ggcgcggcgc
      121 gggcagatcc gccgccctga ccggcagccg gcgcggcagc accggcggac gcgactcgtc
      181 acgcatgaag cgcttcacga tgcgatcctc gagcgagtgg aagctgatca ccgccaggcg
      241 cccacccggc cgcaaccggg acacgcagac gggcagcacg cggctcagct cctcgagctc
      301 ctgattgatg aaaatccgaa gagcctggaa ggtgcgtgtc gccgggtgct gccccggctc
      361 gcgcgtgcgg accgcttttt ccacgagcgc ggcaagttgt ccagtggttg cgacagcccc
      421 ccctgcccga gcagttgcaa tcgcctttgc aatcgcatga gcaaaccgtt cctctccata
      481 ttccctgatg acctccgtga tctggccgac ggacgcctcc gccagccact ccgccacggt
      541 ctgcccgcgg ctcgtatcca tgcgcatgtc gaggggggcg tcgaagcgaa agctcatccc
      601 gcgcgcagcg tcgtccagct ggggcgaaga caccccgagg tccagcagca ctccatccac
      661 cgcctgcacg cccaggcgcg cgagctcgtc gtcgagcgcg ctgaacgccg agtgcacgag
      721 cgtcagccgg gcgtcgccca ccgcctgccc ggcagcgatc gccagcggat cacggtcgaa
      781 ggcgatcaac cgcccctgcg gtccgaggcg ttggagcacc gcgcgactgt ggccgccgcg
      841 cccgaaggtt ccgtcgacgt agaagccgtc ggcccggatc gcaagcgcgt cgaccgcttc
      901 ggagaggagg acgctgacgt gggcatgggc gaggttcaca gcgcgaggct ctcgaacccg
      961 ggcggcaggc cggtattgac cagttccagc atcgcctgct gctgcttctc ccagcgctcg
     1021 tcggaccaca gctcgaagtg cgcaccttgg ccgaccagcc acacctgctt ctcgagcgcg
     1081 gcgaacttgc gcagggaggg cgccaccagc acgcgaccgg cggcgtcgag cgtctcttcc
     1141 tgggcgaagc ccacgagcag gcgcttgagc atcgccgcct gcggatccag gctcggtgcg
     1201 cgcagcacgt ggtcgcggat cggattccag gcggggacgg gatagacgag gcagcagccg
     1261 tgcggatggg cggtcagcac cacctggccg ttgtcgacgg ccagggcatc acgatgccgc
     1321 gcgggaatcg caaggcgtcc cttcgcatcg agattgagcg ccaccgctcc ctggaacatc
     1381 gaaccccctg acggcttcgg gaaagcatcg gaacgacact tttccccact tttttccact
     1441 tgaacccact ttagaggaaa ggccataccg ggtcaaggct gcaagatcgg aatccctttg
     1501 ttgcacaatg acttaccgct ctgcgccaag acacggataa atcctgaaaa atcaagggag
     1561 atgcgcatgg acgttcgcgc ggcacgcgaa atcgggccgc gcaaagggcg cagtgggaaa
     1621 aagtgggaat gccggtgcaa tccggcctgc tcgcactggc ggattccgag cggatggggg
     1681 agaacgctgc tgcctgccgc tcgcagaggg ggcatgcgcg ggcggaaaca ggggaagaag
     1741 tgggaagtcc gccgataagc cgggttctgt cgaacgccac cggcggaccg gtgacatcgg
     1801 gcagtcattc ctctgggcgc cacgtcgccg tggcgctcta gcagcctacc cgggagcgac
     1861 gcgagccacg ccgatgctcc cctatttggc cttgccccgg atggggtttg ccgtgccagt
     1921 cctgttgcca gtcctgcggt gagctcttac ctgccgatgc ttgcgcaccg accctcagct
     1981 tgctcgcgca agccgcggca ccgtttcacc cttacctgta cccattgaag agcaagctct
     2041 tcaaaatgga ctctgctgcc ttgcggcagc gaagtccggg ccatcggcgg tctgctctct
     2101 gttgcacttt ccgtcgcctc acggcgcccg gccgttagcc ggcatcctgc tctgcggggc
     2161 ccggactttc ctccacgcac gacgaggtgc gcagcgactg cctggcggac ttccggcccg
     2221 gattatacgc gtgccggcag ggcgtcgccc ggcttatttg gcggcgggcg cgggtacgaa
     2281 ggcgggcgcg ccgtccttga ggacgaagct gccgacgagg gctccgggcg ggcggtcacc
     2341 cggattgtcc cagtcctgca cgcggcactc ctcggtaccg gcgagatacc aacgcgcgcc
     2401 ctggtgcagc gcccacaggc cgtctccggc ggcgtagatc ttcatctcga cgagggtcgc
     2461 cgagcccgga tggatgcgga tgcgcttctg acaggcgggc aggcgcgaca ccaccatcgc
     2521 ctgacgcaac tcgtcatccc agaagaactt ctgctcgcgc accaggatca gcgcgtgctg
     2581 gttgccgtcg atcatgtagg cggtggcgct gttctcgcaa cccgccagca cggcgagcag
     2641 accgacggca gcgagaccgc ggcgcagcgc gcgcccggcg ccgccgcgga cgccgcgccg
     2701 gatcgacatg ggcgcgcccg catcccgggc gcccgtctca tgcatgcgga cgcccatctc
     2761 aggccccttc caccagcttc cagcccacct gctcgccggc gcgcaagggc accagcggat
     2821 cttcgcccag gtaggggtag ctcgccggca cgctccaggg ctcgcgccgc agcgtgatgg
     2881 tgtcgctgct cggcgccagg ccgtagaagg ccgggccgtt gaggctggcg aaggcctcga
     2941 ggcgatcgag cgcgccggcg gcgtcgaaga cctcggcgta gagctcgatg cccgcgtgcg
     3001 cggtgtagca gcctgcgcag ccgcaggcgt tttccttggt gctgcgcgca tgcggcgcgg
     3061 agtcggtgcc aaggaaaaag cgcgcgttgc ccgaggtgac cgcggcgatc agcgcctcgc
     3121 ggtggctctc gcgcttgagg accggcaggc agtagtggtg cgggcggatg ccgccggcga
     3181 agatcgcgtt gcggttgagc agcaagtggt gggcggtgac cgtggcggcc acgtgcgagc
     3241 ccgctgcgcg cacgaactgc gccgcctccg cggtggtgat gtgctcgaac accaccttga
     3301 gccccgggaa acgccgcacc agcggcgaca gcgtgcgctc gacgaagacg cgctcgcggt
     3361 cgaacacgtc aacctccgcc ccggtggcct cgccatgcac gcacagcacc atgccgagct
     3421 tctccatgcg ttcgagcacc gcatagatct tgtcgatcgc ggtgacgccg gcgtcggagt
     3481 tcgtggtcgc gcccgccgga tagagcttgc aggcgaccac gtggccgctc gcgcgggcgc
     3541 ggtcgatctc gtccggcgac aggttgtcgg tgaggtagag gctcatcagc ggctcgaacc
     3601 tcgacccgcg cgccgcggcg aggatgcgct cgcggtaggc gagcgcctgc gcggtggtgg
     3661 tgaccggcgg cttcaggttg ggcatgatca gcgcccggcc gaagcgggcg gcggtgtgcg
     3721 gcaccaccgc gtcgagcgcg gcgtcgtcgc gcacatgcag gtgccagtcg tcggggcgga
     3781 tcagggtaat cgattgcatc ggggcgttcc tcgtgtgacc cgccgattat aggcgcttcg
     3841 gcggtgcgca cggccgacgc gggcgtgcat gcgtcacgcc tgcggcgaat cctcgccctt
     3901 gcgctcgagc gcgagcgcgt acaaggcctt gcgcggcgcc ccggtgatct cggccgcaag
     3961 ccgcgccgcc cccttcaccg gcagctcggc gagcagcaga tcgagcaccc ggcgcgcccg
     4021 cgcatccacc acctcgccgg ccggcgcgcc ttccaccaca agcacgaact cgccgcgcag
     4081 gcggttggtg tcggcggcga accaggcggc ggtctgcgcc agcggcatgc gcacgatctg
     4141 ctcgtggagc ttggtcatct cgcgtgcgat cagcagctcg cgcgcgccgc cgaggacctg
     4201 ctccatgtcg gcgacgcact cgacgatgcg gtggggcgcc tcgtggaaca ccatcgcggc
     4261 gcgttcgtcg gcgagttcgc gcagcgccgc cgcgcgcgca ccggccttcg ccggcaggaa
     4321 accgacgaag cggaagcccc cctcggcgaa gcccgatacc gacagggccg ccaccgccgc
     4381 gcacggcccg ggcaacggca ccaccggatg ccccgcggcg cgcacccgcg cgaccaggcg
     4441 ggcgcccggg tccgacaccg ccggcgtgcc ggcgtcggtg atcagcgcga cctggcggcc
     4501 ggcagcgagc tcggcgacga tcaccccggc ggcctcgtgc tcgttgtgct ggtgcgccgc
     4561 gagcatgcgc gcgcggatac cgtgcgcatc gagcaggcgc tggctgtggc gcgtatcctc
     4621 ggccgcgacc acgtccaccg cggcgagcac ggcgagcgcg cgcagcgtga tgtcatgcag
     4681 gttcccgagc ggcgtcgcca ccacatacaa tgacggcgta ctggagagcg gacgatcggg
     4741 caagggagcg gagacggtca tgggcgggag atccggaggc tggacgcgca gcgcgggcga
     4801 ggacgcccgg ggcgcgcacg cgggggcgga cagtatcggc gcacgcccga cgccggcgca
//
LOCUS       DAOQNN010000247         4824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10748_length_4824_cov_7.637241, whole genome shotgun sequence.
ACCESSION   DAOQNN010000247 DAOQNN010000000
VERSION     DAOQNN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10748_length_4824_cov_7.637241"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1744)
                     /locus_tag="PKV97_12150"
     CDS             complement(119..1744)
                     /locus_tag="PKV97_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB06676.1"
                     /translation="MISLRNLRLARGAKVLIESATLQIHPGWKVGLTGANGSGKSSLF
                     ALLRDQLHPDQGDLDLPPGWVIAHVAQETPGLAQPAIDYVLDGDAELRRIEAELAAAE
                     AAHDGSHIGELHARLHEIGGYAARARAAALLDGLGFLQSDIERPVADFSGGWRMRLNL
                     AQALMCRSDLLLLDEPTNHLDLDAVIWLEAWLRDYRGTLLLISHDREFLDACVTHIAH
                     IEQQKLGLYTGGYSDFERQRGERLIQQQAMFERQQRDIAHMEDYIRRFRAKATKARQA
                     QSRIKALERMERIAAAHVDTPFSFAFRDAPPAPDPLLQIEDGAVGYGDKTILERITLT
                     LRPGERVGLLGRNGAGKSTLIKLLAHELQLASGRRLEGKGLATGYFAQHQLETLRPDE
                     SALQHMVRLDPQTREQELRDYLGGFDFRGDGVGGTSTPATTPCGPFSGGEKSRLALAL
                     LIWQRPNLLLLDEPTNHLDLATREALSVALNEFEGTVMLVSHDRALLRAVCDEFWLVS
                     RGGIAPFDGDLEDYQRYLLEEAKRAHTEQAVTA"
     gene            1871..2605
                     /locus_tag="PKV97_12155"
     CDS             1871..2605
                     /locus_tag="PKV97_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06677.1"
                     /translation="MSSIAPLGHPNPETARMIRIRSLSLVVAHRSIMPGLCAALLAST
                     ILPAHAQSADEAARTAERTRAEALAADGKALRAEAEATYQATVPGCYEKFLVNRCIDQ
                     AKKQRLETIRRARELEAESRGITLAERQRAAAAKPAAGERPSPPAPPAAPPAAAPLAP
                     SGPSGDATIAPSAEAERIRAQRVEASREAEAATARQRAAQDAERASARRDAEDAAARR
                     ARQAEEDRARYEKRLREYEQEQAGKK"
     gene            complement(2870..3478)
                     /locus_tag="PKV97_12160"
     CDS             complement(2870..3478)
                     /locus_tag="PKV97_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HNB06678.1"
                     /translation="MSRPVVEFWFEFASTYSYLSVMRIERAAAATGVDIEWKPFLLGP
                     VFLALGWNDSPFNIYPPKGRYMWRDLERLAEKEGLPFRHPSRFPRNGLLAARVALVGV
                     EEGWVAPFARAVMSANFAEDREIGEEAVIVEILGALGLPAAELMARAQADANKLALRC
                     QTERAAELGLFGAPSFRVGEELFWGNDRLEDALAWACRADRR"
     gene            complement(3598..4479)
                     /locus_tag="PKV97_12165"
     CDS             complement(3598..4479)
                     /locus_tag="PKV97_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB06679.1"
                     /translation="MHKTELDRLAIGLMVVLCSIWGLQQVAIKLASAGISPVWQAGLR
                     SIGATAIVVVWALLRGVKLFERDRSLAPGLLAGALFAGEFAMIFLGLQHTSASRGVIF
                     LYTAPFFVALGAVWLLPQEHMRRRQWMGMALAFVGVLALFGENLFAATEGAWVGDLML
                     LCAALLWAATTLTVKASALARASAEKTLLYQLGVSALVLPPLSLAMGEPGVFAPTPMV
                     WANLFFQAAIVAGVSYLAWFWLVREYPATRLSSFSFLTPVMGVLAGGLILGEPLTPAV
                     FAALFLVGSGIWVANRS"
     gene            complement(4529..>4824)
                     /locus_tag="PKV97_12170"
     CDS             complement(4529..>4824)
                     /locus_tag="PKV97_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06680.1"
                     /translation="ARWQDELLDALRVRADAAAFAPRLAQALAPREARWSAQHRAAVA
                     HNRARTLELLAEVHALSSAAQRRQLRDEIDMLATQFAGISCAEPARVSAAGGR"
BASE COUNT          794 a         1742 c         1685 g          603 t
ORIGIN      
        1 gggcgcaagc acgcattcga ccagccgtaa ctcaaactcc ccgcagcacg taacctctac
       61 tcccacttac gccgcaccct gaatcgcccc gggaatcgcg gaggccagtt gatttgagtc
      121 aggcagtgac ggcctgctcg gtgtgcgccc gcttggcctc ctccagcaaa tagcgctggt
      181 agtcctccag atccccgtcg aagggggcga tgccgccgcg ggagaccagc cagaattcgt
      241 cgcacaccgc ccgcagcagg gcgcggtcgt ggctgaccag catcacggtg ccttcgaatt
      301 cgttgagggc gacgctgagc gcttcgcggg tggcgaggtc gaggtggttg gtcggctcgt
      361 cgagcagcag caggttgggg cgctgccaga tcagcagcgc cagcgccagg cgcgacttct
      421 cgccgcccga gaacggcccg cagggcgtgg tcgccggggt ggacgtgccg cccacgccgt
      481 cgccgcggaa gtcgaagccg ccgagatagt cgcgcagctc ctgctcgcgc gtctgcggat
      541 cgagcctgac catgtgctgc agcgccgact cgtccgggcg cagcgtttcc agttgatgct
      601 gggcgaagta gccggtcgcc aggcccttgc cttccaggcg gcggccgctg gcgagctgca
      661 gctcgtgcgc gagcagcttg atcagcgtcg acttgccggc accgttgcgc ccgagcaggc
      721 cgacgcgctc gcccgggcgc agggtcagcg tgatgcgctc caggatcgtc ttgtcgccgt
      781 aacccaccgc accgtcctcg atctgcagca aggggtcggg cgctggcggc gcatcgcgga
      841 aggcgaagct gaacggcgtg tcgacgtgtg ccgcggcgat gcgctccatg cgctcgagcg
      901 ccttgatccg gctctgcgcc tggcgggcct tggtggcctt ggcgcggaag cggcggatgt
      961 agtcttccat gtgcgcgatg tcgcgctgct gcctctcgaa catcgcctgc tgctggatga
     1021 ggcgttcgcc gcgctgacgc tcgaagtcgg aatagccccc ggtgtagagg ccgagcttct
     1081 gctgctcgat gtgggcgatg tgggtgacgc aggcgtcgag gaactcgcgg tcgtgcgaga
     1141 tcagcagcag cgtgccgcgg tagtcgcgca gccaggcctc gagccagatc accgcgtcga
     1201 ggtcgaggtg gttggtgggc tcgtcgagca gcagcaggtc ggaacggcac atcagcgcct
     1261 gcgccaggtt caggcgcatg cgccagccgc cggagaagtc cgccacgggg cgctcgatgt
     1321 cggattgcag gaaacccagt ccgtcgagca gggccgccgc gcgcgcgcgc gccgcatagc
     1381 cgccgatctc gtgcaggcgg gcgtggagtt ccccgatgtg cgagccatcg tgcgcagctt
     1441 cggccgccgc cagttcggcc tcgatgcggc gcagctcggc gtcgccgtcg agcacgtagt
     1501 cgatcgccgg ctgcgccagc ccgggggttt cctgcgcgac gtgggcgatc acccagcccg
     1561 gcggcaggtc gaggtcgccc tggtcgggat ggagctggtc gcgcagcagg gcgaacaggc
     1621 tggacttgcc gctgccgttg gcgccggtga ggccgacctt ccagccgggg tggatctgca
     1681 gcgtggcgct ctcgatgagg accttggcgc cgcgcgcgag gcgcaaattg cgaagactga
     1741 tcacgaacgg atacacggcc ggcaggccgc gggcggttgc aaaggcgcta ttctacgcgc
     1801 cggctcgatc gaagccggaa cacgcattcc ggaaacccga gccgaccact cggccgcggc
     1861 aacgcgatga atgtcttcga tcgccccgct cggccacccg aatccggaaa ccgcacgcat
     1921 gatccgtatc cgctccctgt ctctcgtcgt cgcccaccgc tccatcatgc ccggcctgtg
     1981 cgcggcgcta ctcgcctcca cgatcctgcc cgcccacgcg cagagcgcgg acgaggccgc
     2041 ccgcactgcc gagcgtaccc gcgcggaggc gctcgcagcc gacggcaagg cgctgcgcgc
     2101 cgaagccgag gccacctacc aggccaccgt gccgggctgc tacgagaagt tcctcgtcaa
     2161 ccgttgcatc gaccaggcga agaagcagcg cctggagacg atccggcgtg cccgcgagct
     2221 cgaagccgaa agccgcggca tcaccctggc ggagcgccaa cgcgcagcag cggccaagcc
     2281 ggccgccggc gaacgcccca gcccccctgc cccacctgcc gccccgcccg ccgccgcccc
     2341 cctggctcct tccgggccga gcggggacgc gacgatcgcg ccatctgccg aagccgagcg
     2401 catccgcgcc cagcgcgtcg aggccagccg cgaggccgaa gccgccacgg cacgccagcg
     2461 tgcggcccag gacgccgagc gcgcgtctgc gcgccgcgat gccgaagacg ccgccgcccg
     2521 ccgcgcccgc caggccgagg aagaccgcgc gcgctacgaa aagcgcctgc gcgagtacga
     2581 gcaggagcag gcggggaaga agtaagacct gccgcaagcc cgcaatcgag gaccctcggc
     2641 gagacgcagg cgggagcgac gcaagtcgtg ctcgcgcccc gatcgcgact cgcgtcgctc
     2701 ccacaagaaa ccgacacgga tcccgaaacc ccgccaggag cgactcgcgt cgcgcccaca
     2761 agaaaccgac gcggatcccg cagccccgcc aggagcgacg caagtcgcga tacggccccc
     2821 ggaacggcgc cgcgtccgtc gaaccgcccg ctcgcgactc gcatcgcgcc taccggcgat
     2881 cggcacggca cgcccaggcg agcgcatcct ccaggcgatc gttgccccag aacaactcct
     2941 cgccgacacg gaagctcggc gccccgaaca ggcccagttc ggctgcgcgc tcggtctgac
     3001 agcgcagcgc gagcttgttg gcatccgcct gtgcgcgcgc catcagctcc gctgcgggca
     3061 ggccgagcgc gccgaggatc tcgacgatca ccgcctcctc gccgatctcg cggtcctcgg
     3121 cgaaattcgc gctcatcacc gcacgcgcga agggcgccac ccaaccctcc tccaccccca
     3181 ccagcgccac ccgcgcggcg agcaggccgt tgcgcgggaa gcggctgggg tggcggaagg
     3241 gcaggccctc cttctccgcc agacgttcca ggtcgcgcca catgtagcgc cccttgggcg
     3301 ggtagatgtt gaagggcgag tcgttccagc ccagcgcgag gaacaccggc ccgagcagga
     3361 aaggcttcca ttcgatgtcc acgccggtcg cggccgctgc gcgctcgatg cgcatcaccg
     3421 acaggtagga ataggtgctg gcgaactcga accagaactc gacgacgggt cgcgacatgg
     3481 ggaggctcct aggaagacgg tggacgccgt cactataggc gatcgccccg cgccccgccc
     3541 ccccgcgggg cgccaagccc gcacgcgcaa gagagtgcag ctgggcagcc ggtcacgcta
     3601 cgaccggttc gccacccaga tcccgctgcc gacgaggaag agcgcggcga acaccgccgg
     3661 cgtgagcggc tcgccgagga tcagcccgcc ggcgagcacg cccatcaccg gggtcaggaa
     3721 ggagaacgag gacaggcgcg tggccggata ctcgcgcacc agccagaacc aggccagata
     3781 cgacacgccg gcgacgatcg ccgcctggaa gaagagattg gcccacacca tgggcgtggg
     3841 cgcgaacacg cccggctcgc ccatcgccag cgagagcggc ggcagcacca gcgccgacac
     3901 gccgagctgg tagagcagcg tcttctcggc cgatgcgcgc gccagcgcgc tcgccttcac
     3961 ggtcagcgtg gtcgccgccc acagcagcgc ggcgcagagc agcatgaggt cgcccaccca
     4021 ggcgccctcg gtggcggcga agaggttctc gccgaagagc gcgagcacgc cgacgaaggc
     4081 gagcgccatg cccatccact gccgcctccg catgtgctcc tgcggcagca gccagaccgc
     4141 gccgagggcg acgaagaagg gcgcggtgta caggaagatc accccgcgcg aggcgctggt
     4201 gtgctgcagg ccgaggaaga tcatcgcgaa ctcgccggcg aacagcgccc cggcgagcag
     4261 gccgggcgcg aggctgcggt cgcgctcgaa gagcttcacc ccacgcagca gcgcccaaac
     4321 gacgacgatc gccgtggcgc cgatcgagcg caggccggcc tgccacaccg gcgagatgcc
     4381 ggcgctggca agcttgatgg caacctgctg gaggccccag atcgagcaca gcaccaccat
     4441 gaggccgatg gcgagacggt cgagttcggt cttgtgcatg cgggagagcc cgcaccaggg
     4501 cgcgggcggc ggtagacgaa gggcgcgatt atcggccgcc agccgcggaa acgcgagccg
     4561 gttcggcgca gcttatgccc gcgaactggg tggcgagcat gtcgatctcg tcacgcagtt
     4621 ggcggcgttg tgctgcgctg gacaatgcat gcacttcggc gagcagctcc agcgtgcgtg
     4681 cgcggttgtg ggcgaccgcc gcgcgatgct gcgcgctcca gcgcgcctcg cgcggcgcca
     4741 gcgcctgcgc caggcggggg gcgaaggcgg cggcgtccgc gcgcacgcgc aaggcgtcga
     4801 gcagctcgtc ctgccagcgc gcgc
//
LOCUS       DAOQNN010000248         4816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10769_length_4816_cov_9.231884, whole genome shotgun sequence.
ACCESSION   DAOQNN010000248 DAOQNN010000000
VERSION     DAOQNN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10769_length_4816_cov_9.231884"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..2164)
                     /gene="htpG"
                     /locus_tag="PKV97_12175"
     CDS             complement(221..2164)
                     /gene="htpG"
                     /locus_tag="PKV97_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNB06681.1"
                     /translation="MSEVHNPSQPSSQTLAFQAEVKQLLHLMIHSLYSNREIFLRELV
                     SNASDACDKLRFEALDNGALYEDDSELKIRIGFDAEARTITIADNGIGMSRDEAIAHL
                     GTIAKSGTREFFGQLTGDQKKDANLIGQFGVGFYSAFLVADRVTVLTRRAGLAAGEGV
                     KWECSMSGDEAGAYTVEAVDKPARGTEITLHLREGQEDLLSSWKLKSLIRKYSDHIVQ
                     PIVMKKEEWNEEQKKQVVTDEDETVNQANALWTRSKSDITDDEYTAFYKHVAHDFEDP
                     LAWTHSRVEGRHEYTNLLYIPKNAPFDLWDRNARHGIKLYVKRVFIMDDAEKLMPTYL
                     RFVRGVVDSADLPLNVSREILQESKDIDTIRAGCTKKVLTLLESLANSDEAADKDKYA
                     EFWKAFGKVLKEGVGEDFANKDKIAGLLRFASTKLDTPDEVVSLADYIGRMKEGQDKI
                     YFVTAETFNAAKNSPHLEVFRKKGIEVLLLTDRVDEWVTGNLTEFDGKALVSVAKGGL
                     DLGALEDETEKQEVEKAADEYKELLDKMKASLGERVKDVKVTLRLTDSPACLVADEHD
                     LGMNLARILKAAGQNAPVSKPILEINPNHPAVLRLKYEDKHFDDWAAVLFDQALLAEG
                     GTLDDPATFVKRINQLMMAMSGN"
     gene            complement(2255..3889)
                     /locus_tag="PKV97_12180"
     CDS             complement(2255..3889)
                     /locus_tag="PKV97_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB06682.1"
                     /translation="MRFESLTVARRLALMLLVALAGMVLLALAALYENRDDMREGYAR
                     NVRSQIELATGVLAHFHALEQAGVLDREAAQKAAKDTLRGLRYQNNEYFFVLDLELNM
                     LMHPLRPALEGKLADLKDSAGKHFVREMVTLARAQGQGFVDYTWPRSGVATDPAQPKL
                     SYVKAFPSWGWVVGSGVYVDDIDAAFNDAALRFGALVAVVLVLLGLIGVWVIRGVTGA
                     LGGEPAYAGAIMKRAAAGDLAVDVEQRGRGDSLLGNLSNMLAQLRAMIGAIGGSASRL
                     GASAREIHAVSRSVSEASVSQTGATASIAAAIEEMTVSIEQISDSARLAEQHSSTAAG
                     LADEGAGKAERAAREMQAIAARVGDAAQKIQQLVARADEVGTIANVIKEIAGQTNLLA
                     LNAAIEAARAGEQGRGFAVVADEVRGLAERTAVATVQIEKVIEGIQNETRATVGAMAE
                     VSSQVDGGVALVGDATDSLHRIRSTAGEALERIREVAYATGEQSSASTEIARQVQTIT
                     EMADGTSGSMHSVVVAAEQLQGLAAELDALVGRFRC"
     gene            complement(4020..4574)
                     /gene="greB"
                     /locus_tag="PKV97_12185"
     CDS             complement(4020..4574)
                     /gene="greB"
                     /locus_tag="PKV97_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HNB06683.1"
                     /translation="MNKAFVKESDGDDGDEELAPALRLPPGSKNYMTRRGYDALRVEL
                     DQLVRVERPKLVETVAWAAGNGDRSENGDYIYGKKRLREIDRRIRFLIKRIESAEVVD
                     PERQQGLEQVFFGATVSFSDLDTAEEQTLQIVGVDEADASQGRISWISPLARAVLKAR
                     VGDVVRFHSPAGVREIEIGAVEYR"
BASE COUNT          679 a         1687 c         1609 g          841 t
ORIGIN      
        1 tcgccgccta cgcacccgca ccctcggcgt acggctcgta ctacgggttc gcacccgaaa
       61 attgagttgc gcgcgctccc gccgcgccgc gcagcggtga gcgcgcgcgc ctgcgcactc
      121 gcgcagcccg tgcggagcct gcacgactga gctgaaacga acgggcggcc gcccttcggc
      181 gcgccgcccg ttgcgcttgc agtgcccggc cgcagccgga tcagttcccg ctcatcgcca
      241 tcatcagctg gttgatgcgc ttgacgaagg tggccggatc gtcgagcgtg ccgccctcgg
      301 ccagcagggc ctggtcgaac agcaccgccg cccagtcgtc gaagtgcttg tcctcgtact
      361 tcaggcgcag caccgccggg tggttggggt tgatctcgag gatgggcttt gacaccggcg
      421 cgttctggcc cgcagccttg aggatgcgcg cgaggttcat gccgaggtcg tgctcgtcgg
      481 cgaccaggca ggccggggag tcggtcaggc gcagggtcac cttcacgtcc ttgacgcgct
      541 cgcccaggct cgccttcatc ttgtcgagca gctccttgta ctcgtcggcg gccttctcga
      601 cctcctgctt ctcggtctcg tcctccagcg cgcccaggtc cagcccgccc ttggccaccg
      661 acaccagcgc cttgccgtcg aactcggtga ggttgccggt cacccactcg tcgacgcggt
      721 cggtgagcag cagcacctcg atgcccttct tgcggaagac ctcgagatgc gggctgttct
      781 tcgccgcgtt gaaggtctcg gcggtgacga agtagatctt gtcctggcct tccttcatgc
      841 ggccgatgta gtcggcgagc gacaccacct cgtcgggcgt atccagcttg gtcgaagcga
      901 agcgcagcag gccggcgatc ttgtccttgt tggcgaagtc ctcgcccacg ccttccttga
      961 gcaccttgcc gaaggccttc cagaactcgg cgtacttgtc cttgtccgcg gcctcgtcgc
     1021 tgttggccag gctctcgagc agggtcagca ccttcttggt gcagccggcg cggatggtgt
     1081 cgatgtcctt ggattcctgc aggatctcgc gcgacacgtt gagcggcagg tcggccgagt
     1141 cgaccacgcc gcgcacgaag cgcaggtagg tcggcatcag cttctcggcg tcatccatga
     1201 tgaagacgcg cttcacatag agcttgatgc cgtgcctggc gttgcggtcc cacaggtcga
     1261 agggcgcgtt cttcgggatg tagagcaggt tggtgtattc gtggcggccc tcgacgcgcg
     1321 agtgcgtcca cgccagcggg tcctcgaagt cgtgggcgac gtgcttgtag aaggcggtgt
     1381 actcgtcgtc cgtgatgtcg ctcttcgagc gcgtccacag cgcgttggcc tggttgacgg
     1441 tctcgtcctc gtcggtcacg acctgcttct tctgctcctc gttccattcc tccttcttca
     1501 tcacgatcgg ctgcacgatg tgatcggagt acttgcggat caggctcttg agcttccacg
     1561 acgacagcag gtcttcctgg ccctcgcgca ggtgcagggt gatctcggtg ccacgggcgg
     1621 gcttgtcgac ggcctcgacg gtgtaggcgc cggcctcgtc gccgctcatc gagcattccc
     1681 acttcacgcc ttcgcccgcg gccagaccgg cgcgacgggt gagcacggtg acgcgatcgg
     1741 cgaccaggaa ggcggagtag aagccgacgc cgaactggcc gatcaggttg gcgtccttct
     1801 tctggtcgcc ggtgagctgg ccgaagaact cgcgcgtgcc cgacttggcg atggtaccga
     1861 ggtgggcgat cgcctcgtcg cggctcatgc cgatgccgtt gtcggcgatg gtgatcgtcc
     1921 tggcctcggc gtcgaagccg atgcggatct tcagctcgct gtcgtcctca tacagcgcgc
     1981 cgttgtcgag cgcctcgaag cgcagcttgt cgcaggcatc cgaggcgttg gagacgagct
     2041 cgcgcaggaa gatctcgcgg ttggaataca gcgagtggat catcaggtga agcagctgct
     2101 tcacctcggc ctggaaggcg agcgtctggg aggaaggttg cgaagggttg tggacttcgg
     2161 acattggaag ctgctccgtc gaggggttgt cgaaacgcag cagcagttgg gggcacggcg
     2221 gcggatttca agcgccaccg cccccccccg cggatcagca gcggaagcgc ccgaccaggg
     2281 cgtcgagctc ggcggccagg ccctgcagct gctcggccgc caccaccacg ctgtgcatcg
     2341 agcccgaggt gccgtcggcc atctcggtga tggtctgcac ctggcgggcg atctcggtgg
     2401 aggcgctgct ctgctcgccg gtggcgtagg ccacctcgcg gatgcgctcg agcgcctcgc
     2461 ccgcggtgct gcggatgcgg tgcagcgaat cggtcgcgtc gcccaccagc gccacgccgc
     2521 catcgacctg gctcgacacc tcggccatcg cgcccacggt ggcgcgtgtc tcgttctgga
     2581 tgccctcgat caccttctcg atctgcacgg tggccaccgc ggtgcgctcg gccaggccgc
     2641 gcacctcgtc ggccaccacc gcgaagccgc gcccctgctc gccggcgcgc gcggcctcga
     2701 tggccgcatt gagcgcgagc aggttggtct ggccggcgat ctccttgatc acgttggcga
     2761 tggtgccgac ctcgtcggcg cgtgccacga gctgctggat cttctgcgcc gcgtcgccca
     2821 cgcgcgcggc gatggcctgc atctcgcgcg ccgcgcgctc ggccttgccg gcgccctcgt
     2881 cggcgagccc ggccgcggtg ctcgaatgct gctcggccag gcgcgcactg tcggagatct
     2941 gctcgatgct cacggtcatc tcctcgatcg ccgcagcgat cgacgcggtc gcgccggtct
     3001 gcgagaccga ggcctcggac accgagcgcg acaccgcgtg gatctcgcgc gcgctcgcgc
     3061 ccaggcgcga ggcgctgccg ccgatcgcgc cgatcatggc gcgcagctgc gcgagcatgt
     3121 tcgagaggtt gccgagcagg ctgtcgcccc gcccgcgctg ctccacgtcc accgccagat
     3181 cgccggcagc ggcgcgcttc atgatcgcgc cggcgtaggc cggctcgcca ccgagcgcgc
     3241 cggtgacccc gcggatcacc cacacgccga tcagcccgag cagcacgagc acaaccgcga
     3301 ccagggcgcc gaagcgcagc gccgcgtcat tgaaggcagc atcgatgtca tccacataca
     3361 cgcccgagcc caccacccag ccccaggacg ggaaggcctt cacgtagctc agcttgggct
     3421 gggccgggtc ggtggcgacg cccgagcgcg gccaggtgta gtccacgaag ccctgcccct
     3481 gcgcgcgcgc cagcgtcacc atctcgcgca cgaagtgctt gcccgcgctg tccttcaggt
     3541 cggcgagctt gccctcgagc gcgggccgca gcgggtgcat cagcatgttc agctcgagat
     3601 cgagcacgaa gaagtactcg ttgttctggt agcgcagccc gcgcagcgtg tccttcgcag
     3661 ccttctgcgc cgcctcgcgg tcgagcacgc cggcctgctc cagcgcgtgg aaatgcgcga
     3721 gcaccccggt ggccagttcg atctggctgc gcacgttgcg cgcatagccc tcgcgcatgt
     3781 cgtcgcggtt ttcgtacaag gccgcgagcg cgagcaggac catgcccgcg agggcgacga
     3841 gcagcatcag cgccaggcgg cgggcgacgg tgagggattc gaaacgcatc ttgccggacc
     3901 atccacgaaa tggagacttt gcgggagggc tgtcgcctgg gcgtacaacc gcttcgtccg
     3961 gtcaacggca gcattcggcc cgacttgagc accctgcagc ctgaatgcca ctccaccgtt
     4021 cagcggtatt ccaccgcgcc gatctcgatc tcgcgcacgc cggccgggct gtggaagcgc
     4081 accacgtcgc cgacgcgcgc cttgagcacc gcacgcgcca ggggcgagat ccagctgatg
     4141 cgcccctgcg aggcgtcggc ctcgtcgacc ccgacgatct gcagggtctg ctcctccgcg
     4201 gtatcgagat cgctgaagct caccgtcgca ccgaagaaga cctgctccag gccctgctgg
     4261 cgctcgggat cgaccacctc ggcgctctcg atgcgcttga tcaggaagcg gatgcggcgg
     4321 tcgatctcgc gcaggcgctt cttgccgtag atgtagtcgc cgttctccga gcgatcgccg
     4381 ttgcccgcgg cccaggccac ggtctcgacc agcttcgggc gctcgacgcg cacgagctgg
     4441 tcgagctcga cgcgcagcgc gtcgtagccg cgccgagtca tgtagttctt gctgcccggc
     4501 ggcagccgca acgcgggggc gagctcctcg tccccgtcgt cgccgtcgct ctccttcacg
     4561 aaggccttgt tcacttgcgc attccggtcg gtatcgaggg gcgcatggta agaaatcggc
     4621 gcccgccggg gaagcgtcgc ggcgtttccc gccctgggac ggcgcgcgcc tgcggtcgtg
     4681 ttgtcatccc ctgcgccgcc gcataccatt gccggcaaaa cgacgccgcc cggcacttcg
     4741 cccacggccg gcgcacacgc caggggaacg agcatgaccg gggagagctt cgacgaggtg
     4801 accagcctgc gccagc
//
LOCUS       DAOQNN010000249         4789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10873_length_4789_cov_9.014998, whole genome shotgun sequence.
ACCESSION   DAOQNN010000249 DAOQNN010000000
VERSION     DAOQNN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10873_length_4789_cov_9.014998"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKV97_12190"
     CDS             complement(<1..262)
                     /locus_tag="PKV97_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB06684.1"
                     /translation="MGKWLQSNEHLTFEVRERVARITLNRPDRRNALSWEMLSELRAA
                     LLEADDLRSVSVIVLGGAGPDFCAGYDMKSAYARYAAEGDAAL"
     gene            complement(273..1805)
                     /locus_tag="PKV97_12195"
     CDS             complement(273..1805)
                     /locus_tag="PKV97_12195"
                     /inference="COORDINATES: protein motif:HMM:NF009071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNB06685.1"
                     /translation="MSITPMIYLDGRGVTRDEVLRVAQQAATGFEQLGVSEGDTVALL
                     LRNDFAFFEVQQAAAAVGAYGVPLNWHGRTEEVIYVLEDAKPKVLVAHADLLAPIRAL
                     VPAGIRVFVVPTPPEIQQRYGVSTELARPLPGDTVWREWAERFEPLQAPPRRARATMI
                     YTSGTTGHPKGVKRQPATPEESKAYVELFASVYGLKPGMRALVCGPLYHASPNGYGRQ
                     ALMTADVLVLQSKFDPEETLALIEKHRIDNAVMVPTMFVRMLKLPPEVRNRYDVRSLR
                     WVTHTGAPCPREVKKDLMDWWGPVVYETYGGTEVGTATLATPQDWLEHPGCVGMPTPG
                     TRMAFYDEEGNRLPEGEIGEIFARVPAYADFTYLNHEEKRKSVERDGLISLGDVGYMK
                     EGRLYLCDRRSDMVIFGGTNIYPAEIEMVLTQCPGVKDCAVFGIPDDDFGESLAAAVE
                     RMPESQVTPEEIRSYLLERLAKFKVPKRIDFHESLPREDSGKIFKRRLRDPFWQNAGR
                     KI"
     gene            complement(1817..3055)
                     /locus_tag="PKV97_12200"
     CDS             complement(1817..3055)
                     /locus_tag="PKV97_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB06686.1"
                     /translation="MCRAISIRAQRRSTAARPKCRRTSWPRPSSDKANMNFELNEDQA
                     ALAESLSRLLADRYGFEKRRALTASGAAHDEKTWAGLAELGLTALPVPEALGGFGGGA
                     RDLLPAMQAFGRSLSMEPFLASAVLAGTALRLGADEAMQTRLLPGMASGEIVSAWAHD
                     EPAGRHAPLWVETRAERKDGGWKLDGIKSNVPAGGLARYFVVSARIAGAPADANGCAL
                     FLVDAKDAGVALREFRLVDDTAAGELVLTQAAAQPLANPEDSTRTRLAIQGTVAAGIA
                     AVCADMSGAAQAAFDLAMDYLRTRKQFGRLIGENQALRHRASEMLVSLEMARSMAMAA
                     AVAADDASAEDSAPDLHRAKLSVARNARNVALGAIQLHGGIGMTEEYAVGHYLRRIHV
                     LDQMFGDADAHAARLAAALL"
     gene            complement(2961..4121)
                     /locus_tag="PKV97_12205"
     CDS             complement(2961..4121)
                     /locus_tag="PKV97_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB06687.1"
                     /translation="MDLDLTPEQRAFRDEVRTFIREKLPSEIRERLRAGHPPRKQDTV
                     AWQRILNERGWAAPHWPKRYGGADLGPMERLILLDETYRAPAPLPQVLNVGMLGPVLM
                     KFGTPAQCDHFLPRLANLDIWFCQGFSEPGSGSDLASLRTAARREGDHYIVNGQKIWT
                     TTAHLADWVFALVRTDTEARKQEGISFLLIDLRTPGITIRPIHSIDGEHHLNEVFFDE
                     VRVPVGNLVGQENKGWDCAKFLLSNERTGIANVGLCRERLDYARELAGRYTQAGKPLL
                     DDPKLRADIAMLEAETRALEITNWRFLLTDSEQKRLPAFASVLKLKGTELQQEINALL
                     ARIVGPGGLERRSADEVTHGPIVPRYFYSRAASIYGGTTEVQKDILAKAIVG"
     gene            4187..4783
                     /locus_tag="PKV97_12210"
     CDS             4187..4783
                     /locus_tag="PKV97_12210"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNB06688.1"
                     /translation="MTNESLFQPVRTRRGFEAVCDRIREQLMSGELKPGDQLPGEREL
                     AERFGIGRAAARDALRSLEVSGVVEARKGIQGGFFIRNGGSGGLAQTVQDMVSLAQAS
                     LENVMEARTEIICVAIRLACPRATNEEFDTIQRDIDLHADLFRLGRASRNTQSVIEFY
                     RLIAQATHNPLVLMMVDALSEVYRGLLVFSAISDGTKS"
BASE COUNT          762 a         1550 c         1649 g          828 t
ORIGIN      
        1 cgagcgcggc atcgccttcg gcggcgtagc gcgcgtaggc cgacttcatg tcgtagcccg
       61 cgcagaaatc ggggcctgcg cctccgagca cgatcacgct cacgctccgc aggtcgtcgg
      121 cctccagcag cgctgcgcgc agctcggaga gcatctccca cgacagcgca ttgcgccggt
      181 ccggccggtt gagggtgatg cgcgcgacgc gctctcgcac ctcgaaggtg aggtgttcat
      241 tggattgcag ccacttgccc atgcgccgcc cctcaaatct tgcgcccggc gttctgccag
      301 aacgggtcgc gcaagcggcg cttgaagatc ttgccgctgt cctcgcgcgg cagcgattcg
      361 tggaagtcga tccgcttggg caccttgaac ttcgcgagac gttcgagaag ataggaccgg
      421 atctcctccg gcgtgacttg cgactcgggc atgcgttcga cagcggcagc gagcgattcg
      481 ccgaagtcgt cgtcgggaat gccgaacacg gcgcagtcct tcacgccagg gcactgggtc
      541 agcaccatct ctatctcggc cggatagatg ttggtgccgc cgaagatcac catgtccgaa
      601 cggcggtcac agagatagag ccggccttcc ttcatgtagc ccacgtcgcc caggctgatg
      661 aggccgtcgc gctccacgct ctttcgcttc tcttcgtggt tgagataagt gaagtcggca
      721 tacgctggca cgcgagcaaa gatctcgccg atctcgcctt cgggcaggcg atttccctcc
      781 tcgtcataga aggccatgcg cgtgccgggc gtgggcatgc cgacgcaacc gggatgctcc
      841 agccagtcct gcggcgtggc gagcgtggcg gtaccgactt cagtgccgcc gtaggtctcg
      901 tagaccacgg gaccccacca atccatcaga tccttcttca cctcgcgcgg acacggcgcg
      961 ccggtgtgcg tcacccatcg cagcgagcgc acgtcgtagc ggttgcgcac ctccggcggc
     1021 agcttgagca tgcgcacgaa catcgtgggc accatcaccg cgttgtcgat gcggtgcttc
     1081 tcgatcagcg ccagcgtctc ttccggatcg aacttggact gcagcaccag cacgtccgcc
     1141 gtcatcagcg cctgacggcc gtacccgttg ggcgacgcgt ggtagagcgg gccgcacacc
     1201 agcgcacgca tgccgggctt gagcccatac acgctggcga acaactcgac ataggccttg
     1261 ctttcctcgg gcgtggcggg ctggcgcttc acgcccttgg gatggccggt ggtgccggag
     1321 gtgtagatca tggtcgcgcg ggcgcggcgc ggcggcgcct gcagcggctc gaagcgctcg
     1381 gcccattcgc gccaaaccgt gtcgcccggt aagggccgtg cgagttcggt cgagacgccg
     1441 tagcgctgct ggatttccgg cggcgtcggc accacgaaga cgcggatgcc cgcgggcacg
     1501 agcgcgcgga tcggggcgag aaggtcggca tgcgcgacca gcaccttcgg cttcgcgtct
     1561 tcgagcacgt agatcacttc ttccgtgcgg ccgtgccagt tgagcggcac gccataggcg
     1621 ccgaccgcgg cggcggcctg ctgtacttcg aagaaggcga agtcgttgcg cagcagcagg
     1681 gcgacggtgt cgccttcgct cacgccgagt tgctcgaagc ccgtggctgc ctgctgcgcc
     1741 acgcgcagca cctcgtcgcg cgtcacgccg cggccgtcga ggtagatcat gggggtgatg
     1801 ctcatcggtg taggtctcaa agaagggcag ccgcgagacg cgcggcatgg gcgtcggcgt
     1861 cgccgaacat ctggtcgagc acgtggatgc gccgcaggta gtgcccgacg gcgtattcct
     1921 ctgtcatgcc gatgccgccg tgaagctgga tcgcgccgag cgccacgttg cgtgcgttgc
     1981 gggcgacgga gagcttcgcg cgatgcaggt cgggggcact gtcttccgcg ctggcatcgt
     2041 ctgccgcaac agcggcggcc atggccatgc tgcgcgccat ctcgaggctg accagcatct
     2101 cggacgcgcg atggcgcagc gcctggttct cgccgatcag gcggccgaac tgcttgcgtg
     2161 tgcgcaggta gtccatcgcg agatcgaagg cggcctgggc ggcgccgctc atgtcggcgc
     2221 acacggcggc gatgccagcg gccacggtgc cttggatggc gagacgcgtg cgagtcgaat
     2281 cttcggggtt ggccaacggc tgcgccgctg cttgcgtcag caccagctcg cctgctgctg
     2341 tgtcatcgac caggcggaac tcgcgcagcg cgacgcctgc gtccttggcg tccacgagga
     2401 acagcgcgca cccgttcgcg tcagccggtg caccggcgat gcgcgcgctc acgacgaagt
     2461 accgcgccag acctccggcg ggcacgttgg acttgatgcc gtcgagcttc cagccgccgt
     2521 ccttgcgttc ggcacgcgtc tccacccaca gcggcgcgtg acggccggcg ggttcgtcat
     2581 gcgcccaggc cgacacgatc tcgccgctgg ccatgccggg cagcagtcgt gtctgcatcg
     2641 cctcgtcggc gccgaggcgc agggcggtgc ctgcgagcac cgcgctcgcc aggaacggct
     2701 ccatcgagag cgagcggccg aaggcctgca tcgcgggcag caggtcgcgc gcaccgccac
     2761 cgaagccgcc gagtgcttct ggcactggca acgctgtcag gcccagctcg gccaatccag
     2821 cccaggtctt ttcgtcatgc gctgcgccgg aggcggtgag cgcacggcgc ttctcgaatc
     2881 catagcgatc ggccagcagg cgggaaaggc tctccgcgag cgccgcctga tcctcattca
     2941 gttcgaaatt catgtttgcc ttatccgacg atggccttgg ccaggatgtc cttctgcact
     3001 tcggtcgtgc cgccgtagat cgacgctgcg cgcgaataga aatagcgcgg cacaatcgga
     3061 ccgtgcgtga cttcgtcggc ggagcggcgc tccagaccgc ccgggccgac gatgcgtgcg
     3121 agaagcgcgt tgatctcctg ctgcaactcg gtgcccttga gcttgagtac cgacgcgaag
     3181 gccggcaggc gcttctgctc gctgtcggtg agcaggaagc gccagttggt gatctccagc
     3241 gcgcgcgtct cggcctccag catggcgatg tctgcgcgca gcttggggtc gtcgagcagc
     3301 ggcttgccgg cctgtgtgta gcgtccggcg agttcgcgcg cgtagtcgag ccgctcgcgg
     3361 cacaggccga cgttggcgat gcccgtgcgt tcgttcgaca gcaggaactt cgcgcaatcc
     3421 cagcccttgt tctcctggcc gaccaggttg ccaacgggca cgcgcacctc gtcgaagaag
     3481 acttcgttga gatggtgctc gccgtcgatg gagtggatcg gccggatcgt gatgccgggg
     3541 gtgcgcaggt cgatcagcag gaaagaaatg ccttcctgct tgcgcgcctc ggtatcggtg
     3601 cgcacgaggg cgaacaccca gtcggcgagg tgcgcagtgg tggtccagat cttctggccg
     3661 ttgacgatgt agtgatcgcc ctcgcggcgt gcggcagtgc gaagcgacgc gaggtcggag
     3721 ccggagccgg gctccgagaa gccctggcag aaccagatat cgagattcgc gaggcgcggc
     3781 aggaagtggt cgcactgtgc aggcgtgccg aacttcatca gcaccgggcc gagcatgccg
     3841 acgttgagca cctgcggcag cggggcgggg gcgcgatagg tttcgtcgag caggatcagg
     3901 cgttccatcg ggccgaggtc ggcgccgccg tagcgcttgg gccagtgcgg cgcggcccag
     3961 ccacgctcgt tgaggatgcg ctgccaggcc actgtgtcct gcttgcgcgg cggatggccg
     4021 gcacgcaggc gttcgcggat ctctgagggc agcttttcgc ggatgaaggt gcgcacctcg
     4081 tcacggaagg cgcgctgttc gggggtgagg tccaagtcca tggcgaatca tcttatagtt
     4141 caaccaatga atatgcaata ctgccgaacc ctcaaaagga tctttcatga ccaacgaatc
     4201 gctcttccaa cctgtgcgca cccggcgtgg cttcgaggcc gtgtgcgacc gcattcgcga
     4261 acagttgatg agcggcgagc tgaagccggg cgaccagctc cccggcgagc gcgaactggc
     4321 cgagcgcttc ggcatcggac gcgcggcagc acgcgacgca ttgcgcagcc tggaggtctc
     4381 gggcgtggtg gaagcgcgca agggcatcca gggcggcttc ttcatcagga atggcggcag
     4441 cggcggtctt gcgcagacgg tgcaggacat ggtgtcgctc gcgcaggcgt cgctggagaa
     4501 cgtgatggag gcgcgcaccg agatcatctg cgtggcgatc cgcctcgcct gcccgcgcgc
     4561 gaccaacgag gagttcgaca cgatccagcg cgacatcgac ctgcatgcgg atctgttccg
     4621 actggggcgc gcctcgcgca acacgcagtc ggtgatcgag ttctaccggc tcatcgcgca
     4681 agcgacgcac aacccgctgg tgctgatgat ggtggacgcg ttgtctgagg tgtaccgggg
     4741 tctcctcgtt ttcagtgcaa taagtgacgg tacgaaaagc tagcactgg
//
LOCUS       DAOQNN010000250         4782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10903_length_4782_cov_10.185530, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000250 DAOQNN010000000
VERSION     DAOQNN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10903_length_4782_cov_10.185530"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKV97_12215"
     CDS             <1..417
                     /locus_tag="PKV97_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB06689.1"
                     /translation="GKFEMPSRAYIGRFNFKGGDQQKIVGNLSGGERGRLHLAKTLIQ
                     GGNVLLLDEPSNDLDVETLRALEDALLEFAGCALVISHDRWFLDRICTHILAAEGDSQ
                     WFFFEGNYQEYEEDKRKRLGEEGAKPKRIRYKPIAR"
     gene            583..852
                     /locus_tag="PKV97_12220"
     CDS             583..852
                     /locus_tag="PKV97_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06690.1"
                     /translation="MAAGRRIQWQRGQGMTEYIIIVALIAVAAIAVYQYFGQTVRNQT
                     AAIATEIAGKDGGTAKSAAQTAAGKAATEANTARTLDTYTGNAGR"
     gene            882..2306
                     /locus_tag="PKV97_12225"
     CDS             882..2306
                     /locus_tag="PKV97_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HNB06691.1"
                     /translation="MAATPRPGVAQRPQGADRQHGQALVLGLFLVFATLAVLLLMFNT
                     GRVVDEKMRLTNAADATAWSVATLEARALNYDAYTNRAIVANEVAIAQAISLISWMHY
                     FETAVENAPNLNQVAASWLYKPGEYPRLLQLLAGVNGTSYLDVGSGGSLRAIVEGLDI
                     GLGTIATAHDAVANALSLSQRVMHASLDTGIAQAGFANDFVRRIDPDMRASINPISHG
                     FRNLTRTMAKDSAQGDARQRIADVTLRSRDAFTRERVWSIRGLNLPPLQRKVELKRRG
                     GTELIGYDEWRALDTLEHQGQRMKRWRWRWRRTPLAWGGAGFTGDEGADARRGDHGDT
                     YTSNPITTLNRAEPAIVDAGAAHFGGLPETRELRDLSPAAEHRSGVTVHVAKARERLR
                     GSGGIGSVQPQGNLRAFEATAPGGHLAAMARAEVFFERPAARSDNKTERASLYSPYWQ
                     ARLAAVTPTDRAWAAGRQDGMALP"
     gene            2303..3073
                     /locus_tag="PKV97_12230"
     CDS             2303..3073
                     /locus_tag="PKV97_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06692.1"
                     /translation="MKPCHPLLPAAPAAQGGQSTTEFLVLALVLVPLFLSLPLLGKYL
                     DLAHATEQAARYVAFETSIVGPARQPPTAAALAAQVRERIFSTSGAPILSGAGNASAT
                     EHPANPLWIDHRGEHLLADPASRIGVDLAAHANAAPASALLAGPHGLDLPAGSEHTGT
                     IHVSPRDVPGLPPFDTLGLSIRRHQVLLGHNWSAAGSTDYARRIEGAGPLAYPIRPLA
                     LIGNTVGRILPPLVLDKAIEVGRVHAEILPCDRLEQGC"
     gene            3076..3858
                     /locus_tag="PKV97_12235"
     CDS             3076..3858
                     /locus_tag="PKV97_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06693.1"
                     /translation="MPAHPAPRLPTSRHTTPALHPRPRPDGRGRGARAGLSIAFALLV
                     GYHAEAALPEPLPPLAGARITPVLDDARVHGVPTRIRRFEARTATASVRAFYEGAVEG
                     TPLSSRIDGWEVLSWKHGAALRTLRLRPCRTAPAACTEGTLSESDLSRDPAPSSPAPG
                     RPADSLLSTDLETNDAGRHARILVWHDESSPSWAAARLVRELSAQGLALERRAPVSRP
                     GLEGVGLWFGARGREAVATIVRHGSGTTVTLQMIDHGARSGR"
     gene            3855..4034
                     /locus_tag="PKV97_12240"
     CDS             3855..4034
                     /locus_tag="PKV97_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06694.1"
                     /translation="MKRIRRLRGQAMHEYLVILAIVLGVFGLPAFDGKPLLVFFAEAV
                     ATGFARFLASISLPL"
     gene            4042..>4782
                     /gene="cpaB"
                     /locus_tag="PKV97_12245"
     CDS             4042..>4782
                     /gene="cpaB"
                     /locus_tag="PKV97_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HNB06695.1"
                     /translation="MLKRVPRNLIILAVASLFGLGAAWLAARYLEERATEIELRQQGQ
                     MGHAVVAKTDLAVGTPLTRDLVAVREVPVEWLHSSAITPEQFERAAGAVLAQPARGGE
                     PLVWSQLEERRPATLSAQLAPGRRATTIPVDETSSLSGLLRPGDRIDLLATVQRDHHH
                     LTLPLLQGVRVLATGSRTRPGEDDGAVDPLGATFGTLTLDLSEEEATRLVAVRALARL
                     TAVLRHPQDTRAVPALRTDALALLGLAPP"
BASE COUNT          736 a         1823 c         1537 g          686 t
ORIGIN      
        1 ggcaagttcg agatgcccag ccgcgcctac atcggccgct tcaacttcaa gggcggcgac
       61 cagcagaaga tcgtcggcaa cctgtcgggc ggcgagcgtg ggcggctgca cctggccaag
      121 accctgatcc agggcggcaa cgtgctgctg ctcgacgagc cgtccaacga cctcgacgtc
      181 gagaccctgc gcgcgctcga agacgcgctg ctggagttcg ccggctgcgc gctggtgatc
      241 tcgcacgacc gctggttcct cgaccgcatc tgcacccaca tcctggcggc cgagggcgac
      301 tcgcagtggt tcttcttcga gggcaactac caggaatacg aggaagacaa gcgtaagcgc
      361 ctgggcgagg aaggcgccaa gcccaagcgc atccgctaca agccgatcgc gcgctgacac
      421 atccaccgga cgcgcgctgt ccccggaggc ccgctgcatg ccctgcagcg ggcttttgcg
      481 tttacgcctc ccgaggacct cgaacagaga atcttatttg catattgacg acaccattcc
      541 cgtttgtcat tatccgctca tcatcaaccc ggaggatcga tcatggcggc aggcagaagg
      601 atccagtggc agcgcggtca gggcatgacc gagtacatca tcatcgtggc cctgatcgcg
      661 gtcgccgcga tcgccgtgta tcagtacttc ggccagacgg tgcgcaacca gaccgcggcg
      721 atcgccacgg agatcgccgg caaggacggc ggcaccgcca agagcgccgc acaaaccgcg
      781 gccggcaagg ctgcgacgga ggccaacacc gcccgcacgc ttgacaccta caccggcaac
      841 gcgggccgtt gagcctgtcc gaaccgcccg caccggttgc catggccgcc accccgcgtc
      901 ccggcgtcgc gcagcgcccg caaggagccg accgccagca cggccaagca ctcgtcctcg
      961 gtctcttcct cgtctttgcc accctcgcgg tcctgctgct gatgttcaac accggcaggg
     1021 tggtcgacga aaagatgcgc ctcaccaacg ccgccgacgc caccgcctgg agcgtggcca
     1081 cactcgaggc gcgcgcgctc aactacgacg cctacaccaa tcgagcgatc gtcgccaacg
     1141 aggtcgcgat cgcgcaggcg atcagcctga tctcgtggat gcactacttc gagacggcgg
     1201 tcgaaaacgc tccgaacctg aaccaggtcg ccgcgagctg gctgtacaag cccggcgagt
     1261 atccgcgcct ccttcaattg ctcgccgggg tcaatggcac gtcctatctg gatgtcggga
     1321 gcggcggttc gctgcgggcg atcgtggagg gcctggatat cgggctgggt accatcgcca
     1381 ccgcccacga cgcggtcgcg aacgcactct cgctctcgca gcgcgtcatg cacgcgagcc
     1441 tggacacggg catcgcccag gccggtttcg cgaacgactt cgtgcgtcgg atcgatcccg
     1501 acatgcgagc gtcgatcaac ccgatcagcc acgggttccg caatctcacc cgcacgatgg
     1561 cgaaggactc cgcgcaaggc gatgcgcgtc aacggatcgc cgacgtgacg ctacgctcgc
     1621 gcgacgcatt cacccgcgag cgcgtctggt cgatccgcgg cttgaacctc ccgccattgc
     1681 aacgcaaggt cgaactcaag cgacgcggcg gcaccgagct gatcggttac gacgaatggc
     1741 gcgcgctcga cacgctcgaa caccagggcc agcggatgaa acgctggcgc tggcgctggc
     1801 gtcgcacgcc gctcgcctgg ggcggtgccg gctttacggg cgacgagggt gccgatgccc
     1861 ggcgtggcga tcacggcgac acctacacga gcaatccgat cacgacgctg aaccgggccg
     1921 agcccgcgat cgtcgatgcg ggggccgcgc acttcggcgg gctgccggag acacgcgagc
     1981 tgcgcgacct ttcgcccgcc gcagagcacc gctccggcgt gaccgtccac gttgccaagg
     2041 cacgggaaag actgcgcggt tcggggggca tcggcagcgt gcaaccgcag ggaaacctgc
     2101 gtgcattcga agcgaccgcg ccgggcggcc acctcgccgc gatggcgcgc gccgaggtgt
     2161 tcttcgagcg tcccgccgcg cgcagcgaca acaagaccga gcgggcaagt ctgtacagcc
     2221 cctactggca ggccaggctc gccgccgtca ccccgacgga ccgcgcctgg gctgccggac
     2281 ggcaggacgg gatggcgctg ccatgaagcc ctgccacccc ctcctcccgg ctgcgcccgc
     2341 agcgcaaggc ggccagtcga caaccgagtt cctcgtgctc gcgctggtcc ttgtcccgct
     2401 cttcctgagc ctgccgctgc tcggcaagta cctcgacctc gcccacgcga ccgagcaggc
     2461 ggcacgttac gtggccttcg agacctcgat cgtgggccct gcccggcagc cccccacggc
     2521 cgcagccctg gccgcgcagg tccgcgagcg catcttctcc acaagcggag cgccgatcct
     2581 cagcggtgcc ggcaacgcgt ccgcgacgga gcatccggcc aaccccttgt ggatcgatca
     2641 tcgcggggaa catttgctcg ccgacccggc cagccggatc ggggtcgatc ttgcggcgca
     2701 cgccaacgcg gcaccggcct ccgcgctgct cgccggcccg cacggcctcg acctgccggc
     2761 cggctccgaa cacaccggga cgatccacgt gtcgccgcgc gacgttcccg gcctgccccc
     2821 cttcgacacc ctcggactgt cgatccgccg ccaccaggtc ctgctcggtc acaactggtc
     2881 ggcggccgga tcgaccgact acgcccgccg catcgaaggc gccggcccgc tcgcctaccc
     2941 gatccggccg ctggcgctga tcgggaatac ggtcgggcgc atcctgccgc cgctggtgct
     3001 cgacaaggcg atcgaggtcg ggcgcgtgca cgccgagatc ctgccttgcg accgtctcga
     3061 acaagggtgc tgaccatgcc cgcacacccc gccccgcgcc tcccgacttc gcgccacacg
     3121 actcctgccc tccatccccg gcctcgtccg gacggacgtg gtcgcggcgc gcgcgcaggc
     3181 ctgtcgatcg ccttcgcgct gctggtcggt taccacgccg aggccgccct gcccgaaccc
     3241 ttgccgcccc tcgccggagc ccgtatcacg ccggtgcttg acgacgcccg cgtccatggc
     3301 gtgccgaccc ggatccgccg gttcgaggcg cgaaccgcga cagccagcgt gcgcgccttc
     3361 tacgagggag cggtcgaggg cacgcccctg agctcgcgca tcgacggctg ggaagtgctg
     3421 tcctggaagc acggcgcggc cttgcgcacc ttgcgcctgc gtccatgccg tacggcgccg
     3481 gccgcctgca ccgaaggcac cctgtccgag tcagatctct cgcgcgatcc ggcgccatcc
     3541 tcgccagctc cgggccgccc ggcggacagc ctgctcagca ccgacctgga aacgaacgat
     3601 gccggccggc atgcacgcat cctcgtctgg catgacgaat cctcgccctc ctgggccgcg
     3661 gcgcgcctgg tccgcgagct ctcggcccag ggcctcgcgc tcgagcgtcg tgcgccggtc
     3721 tcccggcccg gcctggaggg cgtggggctg tggttcggcg cccggggccg cgaggcggtt
     3781 gcgaccatcg tccgccacgg cagcgggacc acggtgacgc tgcagatgat cgaccacggc
     3841 gcacggagcg ggcgatgaag cggatccggc gcctccgcgg ccaggcgatg catgaatacc
     3901 tggtcatcct cgccatcgtg ctcggcgtct tcggccttcc ggccttcgac ggcaagccgc
     3961 tcctcgtctt cttcgccgaa gccgtggcca ccggcttcgc gcgttttctc gccagcattt
     4021 cactgccgtt gtgatccacc catgctcaag agagtccccc gcaacctcat catcctggcc
     4081 gtcgcctccc tcttcggcct tggcgcagcg tggctcgccg cacgctacct ggaggagcgc
     4141 gccaccgaga tcgaactgcg ccagcaaggg cagatgggcc atgcggtggt cgccaagacg
     4201 gacctcgcgg tcgggacccc cctcacccgc gacctcgtcg ccgtgcgcga agtgccagtc
     4261 gagtggctgc actcgagcgc gatcaccccg gagcagttcg agcgcgcggc aggtgcggtg
     4321 ctcgcccagc cggctcgcgg tggcgagccg ctggtgtggt cgcagctcga agagcggcga
     4381 cccgccaccc tctccgcgca gctcgccccg ggtcggcgcg ccaccacgat tccggtcgac
     4441 gagaccagct cgctctccgg cctgctgcgc ccgggcgacc gcatcgacct gctcgcgacc
     4501 gtgcagcgcg accaccatca cctgaccctg cccctgctcc agggcgtacg cgtgctcgcg
     4561 accgggagcc gcacccggcc cggcgaggac gacggcgcgg tcgacccgct cggcgccacg
     4621 ttcggcacgc tgaccctgga cctctcggaa gaggaggcga cccggctcgt cgccgtgcgc
     4681 gcgctcgccc gcctgaccgc ggtgctccgc catcctcagg acacacgtgc ggtcccggcg
     4741 ctgcggaccg acgcgctcgc gctcctcggc ctcgcaccac cg
//
LOCUS       DAOQNN010000251         4773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10933_length_4773_cov_7.860110, whole genome shotgun sequence.
ACCESSION   DAOQNN010000251 DAOQNN010000000
VERSION     DAOQNN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10933_length_4773_cov_7.860110"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PKV97_12250"
     CDS             <1..949
                     /locus_tag="PKV97_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HNB06696.1"
                     /translation="FGAHPSLEVALERSLTELLQGRSFEGLNDLPRPTFESNAVTEPN
                     NFVEHFIDSSGVVSWRFFSAKADYPFVEWDFSSHGERSNAEEAATLFGILEDMGKEVY
                     MAVFDQLGATVCRIVVPGYSEVYPVDDLIWNNTNKALAFRADILNLHRLDDEALEALL
                     ERLEESELDDYTDIIELIGIEFDENTVWGQLTILELKLLIHLALQQLEEAKERVEAFL
                     QYNDNTVDRVLFYQALNVVLEVMLDDELALEDYEANFRRMFGDARMDAVIGSVEGSVR
                     FHGLTPTSLQLEGLERHQRLIDSYRKLHKARARAAGVSA"
     gene            952..1989
                     /locus_tag="PKV97_12255"
     CDS             952..1989
                     /locus_tag="PKV97_12255"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNB06697.1"
                     /translation="MLLPETDPPASVLVDFIDGVGVLTLNRPAVLNALDLATVRALTR
                     QLDAWADDPAIRAVLLRGSGERAFCAGGDVRSLYQLQRQGAPELVSYFDEEYALDCRI
                     HRYPKPTIALMDGVVMGGGMGISQGCTHRLVTERTRMAMPETAIGIFPDVGASYFLSR
                     LPPALGVYIGVVGDTLSGADAVASGLADRLVPSALFADLRALIAAIDAEAWPAAPTQT
                     KIPSLLTAPVLAAVERHFGHGSVEEILAALAGETDPLLRPWVERTQGLLAQRSPLMLC
                     VTFAQLQRGRRLGLEDCFRMERTMMEYSFAHGDTMEGIRALLIDKDRNPRWRHRTVAE
                     VGREEVEAFFG"
     gene            complement(2261..3172)
                     /gene="fdhE"
                     /locus_tag="PKV97_12260"
     CDS             complement(2261..3172)
                     /gene="fdhE"
                     /locus_tag="PKV97_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HNB06698.1"
                     /translation="MNTQAIELPPSGEAAAVLLPDPGTVFLARAERFDALALHHGLGE
                     WLAFLGRLSHAQHAALQASAQDITPPAEDVLERAAKHRMPPISVASYQRPAVWREILR
                     NLVGDLLENAPVGARETLDGLLVTSAEELDKLGEKLLNGEPEGADMARLPYVAAAMQV
                     VFTALASKLDAGALPLMEAHSTCPVCGSHPVVSVIRSDGPTAGLRYVHCTLCNTEWHV
                     PHATCVSCGDRDKITLHEIDGDDGIARAESCGSCESYTKLIVQPKNVRVDPIADDLAS
                     VALDMLVDDAGFVRSGPNYLLIGAGES"
     gene            3461..4258
                     /locus_tag="PKV97_12265"
     CDS             3461..4258
                     /locus_tag="PKV97_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNB06699.1"
                     /translation="MNAPPSVSRSDSRPVSPRVALFATCLVDAMRPSVGFAALKLLRD
                     AGCRVEIPQAQTCCGQPAFNSGDTAHAATLARHFIETFEGYDYVVAPSGSCIGMTKVH
                     YAEALADDPAWAERARRLGERSFELMSFLVDVMGYAPPDVKLDTSFTYHDSCSGLREL
                     GVHDQPRALLGQVAGLTLKPLEGNDVCCGFGGTFCVKYAAISNAVVGEKAEAIERSGA
                     QLLLGGDLGCLMNMAGKLKRKGSSVRAFHAAEVLAGMGGGPAIGEEG"
     gene            4262..>4773
                     /locus_tag="PKV97_12270"
     CDS             4262..>4773
                     /locus_tag="PKV97_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNB06700.1"
                     /translation="MEEATLSFKARAERALADPTLKIAIDRTTGTAERKRAVAMSQFP
                     QFEAARERGKRIKDHVIANLDHYLLEFERNAIASGAKVYWAATAEEASQIVVRLCKEA
                     GAKRVTRVKSMLGEEIGLPHALDEAGIERVETDLAEHIVQLGGDRPSHIVWPSMHRTR
                     EQVSEMFKRL"
BASE COUNT          744 a         1650 c         1652 g          727 t
ORIGIN      
        1 gttcggcgcg cacccgagcc tcgaggtggc gctggagcgc agcctgaccg agctgctgca
       61 ggggcgtagc ttcgagggcc tcaacgatct gccgcggccc actttcgaga gcaacgccgt
      121 caccgagccg aacaacttcg tcgagcactt catcgattcg agcggcgtgg tgtcgtggcg
      181 cttcttcagc gccaaggccg attacccgtt cgtcgaatgg gatttctctt cccacggtga
      241 aaggtccaac gccgaggaag ccgcgaccct gttcggcatc ctcgaggaca tgggcaagga
      301 agtgtacatg gcggtgttcg accagctcgg cgccaccgtc tgccgcatcg tcgtgccggg
      361 gtattccgag gtctatccgg tggatgacct gatctggaac aacaccaaca aggcgctggc
      421 cttccgcgcc gacatcctca acctgcatcg cctcgacgat gaggccctcg aagccctgct
      481 cgagcgcctg gaagaaagcg agctcgacga ttacaccgac atcatcgagc tgatcggcat
      541 cgagttcgac gaaaacacgg tgtggggtca gctcacgatc ctggagctga agctgttgat
      601 ccatctcgcc ctgcagcaat tggaagaggc gaaggagcgc gtcgaggcct tcctgcaata
      661 caacgacaac acggtcgatc gcgtgctgtt ctaccaggcc ctgaacgtgg tgctggaggt
      721 gatgctggac gacgagctgg cgctggaaga ctacgaggcc aacttccgcc gcatgttcgg
      781 cgacgcgcgc atggacgcgg tgatcggctc ggtggagggc agcgtgcgtt tccacggcct
      841 cacgccgacg agcctgcagc tcgaagggct cgagcgccac cagcgcctta tcgacagcta
      901 ccgcaagctg cacaaggcgc gggcgcgcgc ggcgggcgtg tccgcgtaga catgctgctc
      961 cccgagaccg atcctcccgc cagcgtcctg gtcgacttca tcgacggcgt cggcgtcctc
     1021 accttgaatc gcccggcggt gctcaacgcg ctcgacctcg ccaccgtgcg cgcgcttacc
     1081 cgccagctcg acgcctgggc ggacgacccg gcgatccgcg cggtgctgct gcgcggcagc
     1141 ggcgagcgcg cgttctgcgc cggcggcgac gtgcgctcgc tgtaccagct gcagcgccag
     1201 ggggcgccgg agctggtgag ctacttcgac gaggagtacg cgctcgactg ccggatccac
     1261 cgctacccca agcccaccat cgccctcatg gacggcgtgg tgatgggcgg cggcatggga
     1321 atctcgcagg gttgcaccca tcgcctggtc accgagcgca cgcgcatggc gatgccggag
     1381 acggcgatcg ggatctttcc cgacgtgggc gcgagctact tcctgtcgcg cctgccgccg
     1441 gcgctgggcg tctacatcgg ggtggtcggc gacaccctga gcggcgccga cgcggtggcg
     1501 agcggcctcg ccgaccgcct ggtgccgagc gccttgttcg ccgacctccg ggcgctgatc
     1561 gccgcgatcg atgccgaggc ctggccggcg gcgccgacgc agacgaagat cccctcgctg
     1621 ctgaccgcgc cggtgctcgc cgcggtggag cgccacttcg gccatggcag cgtggaagag
     1681 atcctcgccg cgctcgccgg cgagaccgat ccgctgctgc ggccgtgggt ggaacgcacg
     1741 caggggctgc tggcgcagcg ttcgccgctg atgctgtgcg tgaccttcgc gcagctgcag
     1801 cgcggacgcc ggctgggcct ggaggactgc ttccgcatgg agcggacgat gatggagtac
     1861 agcttcgcgc acggcgacac catggaaggc attcgcgcgc tgctgatcga caaggaccgc
     1921 aacccgcgct ggcggcatcg cacggtggcg gaggtcggcc gcgaagaggt ggaggccttc
     1981 ttcggctgaa gggcagactc gtcgccgccg caatcgcgac tcgcgtcgct cttacaagac
     2041 cgccgctgct ggcggtcggt cccagcggta ggagcgacgc aagtcgcgat cctccgcgcc
     2101 cgaaacccgc gccatcgccg ccgtaccgcc gcaatcgcga ctcgcgtcgc tcctacaaaa
     2161 ccgccgcggc tggcggtcgg ccccagcggt aggagcgacg caagtcgcga tcctccacgc
     2221 ccgaaacccg cgccgccgcc cccgcactgc cttggcgttg ttacgactcc ccggcgccga
     2281 tgagcaggta attcggcccg ctgcggacga aacccgcgtc gtcgaccagc atgtccagcg
     2341 ccaccgaggc gaggtcgtcg gcgatcgggt cgacgcgcac gttcttcggc tggacgatga
     2401 gcttggtgta gctctcgcag gacccgcagg actccgcgcg cgcgatgccg tcgtcgccgt
     2461 cgatctcgtg cagggtgatc ttgtcgcggt cgccgcagct cacgcaggtg gcgtgcggga
     2521 cgtgccactc ggtgttgcac agcgtgcaat gcacgtagcg caggccggcg gtcgggccgt
     2581 cgctgcggat cacgctcacg accgggtggc tgccgcacac cgggcaggtg ctgtgggctt
     2641 ccatcagcgg cagcgcgccg gcgtcgagct tgctggcgag cgcggtgaac accacctgca
     2701 tcgccgccgc gacgtagggc agacgggcca tgtcggcgcc ctcgggttcg ccgttgagca
     2761 gcttctcgcc cagcttgtcg agctcctcgg cgctggtgac gagcagcccg tcgagcgtct
     2821 cgcgcgcgcc cacgggcgcg ttctccagca ggtcgccgac gaggttgcgc aggatctcgc
     2881 gccacaccgc ggggcgctgg tacgaggcca cgctgatcgg cggcatgcgg tgcttggctg
     2941 cgcgttcgag gacgtcttcc gccgggggcg tgatgtcctg tgccgaggct tgcagcgccg
     3001 catgctgggc atgcgacagc ctgccgagga aggcgagcca ctcgcccagg ccgtggtgca
     3061 gcgcgagggc gtcgaaacgc tcggcacgcg cgaggaagac ggtgcccgga tcgggcagga
     3121 ggacggcggc ggcttcgccc gagggcggca gttcgatggc ttgggtgttc atgttgtctc
     3181 tggatggaaa agcgcacgcg atgatcggcg cagtagcgtg ccgaatgcaa gccggacatg
     3241 cagaaaccac cctccgctgg aggtcgcgac ggccttgcag gcgtgacgcc ggggcccttc
     3301 agggtttgcc cgtagaccgg tcccgaatcc gcgccgcacc acgaaaaaca atcgtattct
     3361 gtccgacgtg gcgaggaggc ggtgctgccg gggcctggcc cctttgcggt gcgccgaacg
     3421 cccgcggacg atcgcccgga caacctggag gaagaccccc atgaacgcac ccccaagcgt
     3481 ctcgcgctcc gattcgcgcc cggtctcgcc gcgcgtcgcg ctgttcgcca cctgcctggt
     3541 ggacgcgatg cgccccagcg tcggcttcgc cgcgctcaag ctgctgcgcg acgccggctg
     3601 ccgggtcgag atcccgcaag cgcagacctg ctgcggccag cccgccttca acagcggcga
     3661 taccgcgcac gcggccacgc tggcacgcca tttcatcgag accttcgagg gctacgacta
     3721 cgtggtcgcg ccctcgggct cctgtatcgg catgaccaag gtgcactacg ccgaggcgct
     3781 cgccgacgac ccggcctggg ccgagcgcgc acgccgcctg ggcgagcgca gcttcgagct
     3841 gatgagcttc ctggtcgacg tgatgggcta cgcccccccc gacgtgaagc tcgacaccag
     3901 cttcacctac cacgacagct gctcgggcct gcgcgagctc ggcgtgcacg accagccgcg
     3961 tgcgctgctc ggtcaggtcg ccgggctcac cctgaagccg ctcgaaggca acgacgtgtg
     4021 ctgcggcttc ggcggcacct tctgtgtcaa gtacgcggcg atctccaacg cggtcgtcgg
     4081 cgagaaggcc gaggccatcg agcgcagcgg cgcgcagctg ctgctcggcg gtgaccttgg
     4141 ctgcctgatg aacatggccg gcaagctcaa gcgcaagggc tcgagcgtgc gcgccttcca
     4201 cgccgccgag gtgctcgccg gcatgggcgg cggtccggcc atcggcgagg agggctgagc
     4261 catggaagag gccacgctct ccttcaaggc gcgcgccgag cgtgcgctcg ccgaccccac
     4321 gctcaagatc gccatcgacc gtaccaccgg caccgccgag cgcaagcgcg cggtggcgat
     4381 gagccagttc ccgcagttcg aggcggcgcg cgagcgcggc aagcgcatca aggatcacgt
     4441 gatcgccaac ctcgaccact acctgctcga gttcgagcgc aacgccattg cctcgggcgc
     4501 caaggtgtac tgggccgcga ccgccgagga ggcctcgcag atcgtcgtgc gcctgtgcaa
     4561 ggaggcgggc gcgaagcgcg tgacgcgcgt caagtcgatg ctgggcgagg agatcggcct
     4621 gccgcacgcc ctggatgaag ccggcatcga gcgcgtcgag accgacctcg ccgagcacat
     4681 cgtgcagttg ggtggcgacc gtccctcgca catcgtgtgg ccgtcgatgc accgcacgcg
     4741 cgagcaggtc tcggagatgt tcaagcgcct gca
//
LOCUS       DAOQNN010000252         4767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_10957_length_4767_cov_10.164686, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000252 DAOQNN010000000
VERSION     DAOQNN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10957_length_4767_cov_10.164686"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1566)
                     /locus_tag="PKV97_12275"
     CDS             complement(<1..1566)
                     /locus_tag="PKV97_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNB06701.1"
                     /translation="MSIDRHTLELLAPAKTADFGIEAIDHGADAVYIGGPAFGARSSA
                     DNSVEDIARLVQHAHRYHAEVFVATNTILFDHEIEPAHKLIWQLYDAGVDALIVQDMG
                     LLELDLPPIQFHASTQTDIRDASKARFLQDVGFSQIVLARELSLNEVKKIAAATTCQL
                     EYFVHGALCVAFSGQCYISHAHTGRSANRGECSQACRLPYDLKDKDGHTVASNQHMLS
                     MKDNNQSANLRALAAAGVSSFKIEGRYKDLSYVKNITAHYRTLIDEILEHPDNDGARY
                     RRASSGRTTFFFTPQADKTFNRGYTDYFTNDRRHGIEAFESPKFVGEPIGRVKKIDTK
                     GRTFFDVERAAPIHNADGLTWYDPKGELTGLRVNRAEADGGGEGIDRIFPADPLPTDL
                     VPGTSLFRNHDHEFERALGKKSAERRIRVDARFAATHDGFALTLTDEDGVAVTATLAA
                     AFEPAQNVERALATLREHLGKLGNTIFSAGELVLDLPAAPFLPAGQLNALRRDAVERL
                     EAGRLAAHARPLRA"
     gene            complement(1704..2582)
                     /locus_tag="PKV97_12280"
     CDS             complement(1704..2582)
                     /locus_tag="PKV97_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06702.1"
                     /translation="MLHHPRIALVTGATMAKPDPETHLLVDALAQRGIVADVLPWNSA
                     QDWAAYPLVVVRTPWDYFRHLDAFLAWAERTSALTHLVNPVAVIAWNSHKGYLRELAA
                     GGVATVPTLWIERGEAAPGARLTAQGWDEVVVKPAVSIGAIGALRARADDPACGAHLE
                     DLVAAGDVMVQPFVHSIAEAGEVSMIYFGGVFSHAICKRPAAGDYRVQDLYGGTVHAH
                     TPTAAERRLAEAVLAQAPAATTYARVDLVSHEGRPALMELEAIEPELFLGATPDAAAN
                     FAAELARRLEVAQAQG"
     gene            complement(2645..3001)
                     /locus_tag="PKV97_12285"
     CDS             complement(2645..3001)
                     /locus_tag="PKV97_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06703.1"
                     /translation="MPTSVRTLLRVLFIAALGGAFWLALMPIEPVIKLFSWQDKVEHA
                     LLFAGLAILGVAAWPQRVGLLAAGLLAYGAAMEVAQSTTAYRVGDPLDWLADAVGLLV
                     LLPFLRNRKTNTANAR"
     gene            3164..4342
                     /locus_tag="PKV97_12290"
     CDS             3164..4342
                     /locus_tag="PKV97_12290"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNB06704.1"
                     /translation="MQDGKVRELSSSRTRAALRVALLAGMLAAGSMHAPLAGATGMVY
                     PSETGPLRVTEVARGLESPWALAFLPDGRMLVTERPGRMRIVDTAGRVSEPIAGVPEV
                     HARGQGGLLDVALAPDFDRDRLIVFSYAEPTARGARTAVARARLDVDGLRLEDVRRIF
                     AQDEDPAGNHHWGSRLVFGRDGRLFVTLGDRFHHRERAQALDSHLGKVVRIDPDGGVP
                     ADNPLVGRAGVRGEIWSWGHRNVQGAALHPRTGELWTHEHGPQGGDEINRTLGGRNYG
                     WPEITYGREYVTGRKIGAGSEREDVAAPVLQWTPSIAPSGMAFYTGDAFPQWQGNLFV
                     GALKFQLLARLVLDGERVVREERLLEGLGRIRDVRQGPDGRLWLLDESAGRVLRIDPQ
                     "
     gene            complement(4391..>4767)
                     /locus_tag="PKV97_12295"
     CDS             complement(4391..>4767)
                     /locus_tag="PKV97_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNB06705.1"
                     /translation="RDARSNAMCGRFGFGPEVGELAKQLRFSLSAQPDNVFNVATARG
                     VDVLISDVDDPKIAARVPAWYRRSVAARTFVLFPLTIKGRPVAMIYADKDRAGELRIG
                     EQELAMLRTLRNQAVLAIKQAG"
BASE COUNT          656 a         1534 c         1827 g          750 t
ORIGIN      
        1 ggcccgcagc gggcgggcgt gggccgcgag gcggccggcc tcgagccgct ccacggcatc
       61 gcggcgcagt gcattgagct gtccggcggg caggaagggc gcggcgggca ggtcgagcac
      121 gagctcgcct gcgctgaaga tcgtgttgcc cagcttgccg aggtgctcgc gcagggtggc
      181 gagtgcacgc tcgacgttct gtgccggctc gaaggcggcg gcaagcgtcg cggtgacggc
      241 gacgccgtcc tcgtcggtca gggtcagcgc gaagccgtcg tgggtggcgg cgaaacgtgc
      301 atcgacacgg atgcggcgct cggccgactt cttccccaac gcgcgctcga attcgtggtc
      361 gtggttgcgg aacagcgagg tgccggggac caggtcggtc ggcagggggt cggcagggaa
      421 gatgcggtcg atgccttcgc cgccgccgtc ggcctcggcg cggttcaccc gcaggccggt
      481 gagctcgccc ttggggtcgt accaggtcag gccgtcggcg ttgtggatcg gcgccgcgcg
      541 ctcgacgtcg aagaacgtgc gccccttggt gtcgatcttc tttacgcggc cgatgggctc
      601 gccgacgaac ttgggcgatt cgaacgcctc gatgccgtgg cggcgatcgt tggtgaagta
      661 gtccgtgtag ccgcggttga aggtcttgtc ggcctgcggg gtgaagaaga aggtggtgcg
      721 tccgctggag gcgcggcgat accgggcgcc gtcgttgtcc gggtgctcga ggatctcgtc
      781 gatcagcgtg cggtagtgcg cggtgatgtt cttgacgtag gagagatcct tgtagcggcc
      841 ctcgatcttg aacgagctta cccccgcggc ggcgagcgcg cgcaagttgg cgctctggtt
      901 gttgtccttc atcgacagca tgtgctggtt gctggcgacg gtgtggccgt ccttgtcctt
      961 gaggtcgtag ggcagccggc aggcctgcga gcactcgccg cggttggcgc tgcgcccggt
     1021 gtgcgcgtgg ctgatgtagc actggccgga gaaggccacg cacagtgcgc cgtgcacgaa
     1081 gtattcgagc tggcaggtgg tggccgcggc gatcttcttc acctcgttca acgacagctc
     1141 gcgcgccagc acgatctgcg agaagcccac gtcctgcagg aagcgcgcct tgctcgcgtc
     1201 gcggatgtcg gtctgagtgc tggcgtggaa ctggatcggc ggcaggtcga gctcgagcag
     1261 gcccatgtcc tgcacgatga gtgcgtcgac gccggcatcg tacagttgcc agatcagctt
     1321 gtgcgccggc tcgatctcgt ggtcgaagag gatggtgttg gtggcgacga agacctcggc
     1381 gtgataacgg tgggcgtgct gcacgaggcg cgcgatgtcc tccaccgagt tgtccgcgga
     1441 ggagcgcgcg ccgaaggccg ggccgccgat gtagaccgcg tccgcgccgt ggtcgatggc
     1501 ctcgatgccg aagtcggcgg tcttggcggg ggcgaggagt tcgagggtgt ggcggtcgat
     1561 gctcatcggt ggttgaagtg cgcaagcgct tgattggaaa ggccgcgatg ataacaaggt
     1621 gtgcgccggc aggcgcgccc gaccgcaaaa aaggcgcgcc gccggtgctg gcggcggatc
     1681 gcccggtgcg cccgtgcccg cgtctatcct tgcgcctgcg ccacttcgag ccggcgtgcc
     1741 agttcggcgg cgaagttcgc ggccgcgtcc ggggttgcgc cgaggaagag ttcgggctcg
     1801 atcgcctcca gctccatcag cgccggccga ccctcgtggc tcaccaggtc gacccgggca
     1861 taggtggtcg ccgcgggcgc ctgcgcgagc acggcctcgg ccagcctgcg ctcggccgcg
     1921 gttggcgtgt gcgcatgcac cgtgccgccg tacaggtcct gcacacgata gtcgcctgcg
     1981 gcagggcgtt tgcagatggc atgggaaaag accccgccga agtagatcat cgacacctcg
     2041 cccgcctctg cgatgctgtg cacgaagggc tggaccatga cgtcgcccgc tgcgaccagg
     2101 tcctccagat gcgcgccgca ggcgggatcg tcggccctgg cgcgcagggc gccgatggcg
     2161 ccgatggaca ccgcaggctt gaccaccacc tcgtcccagc cctgcgcggt cagccgtgcg
     2221 ccgggggcgg cttcgccgcg ttcgatccac agcgtgggca cggtggcgac gccgccggcc
     2281 gcgagttcgc gcaggtagcc cttgtggctg ttccacgcga tcacggctac cgggttgacg
     2341 agatgggtga gcgcgctggt gcgctcggcc caggccagga acgcgtcgag gtggcggaag
     2401 tagtcccatg gcgtgcgtac cacgacgagg gggtacgcgg cccagtcctg tgcgctgttc
     2461 cagggcagca cgtcggcgac gatgccgcgc tgcgcgaggg cgtcgacgag caggtgggtt
     2521 tcgggatccg gcttggccat cgtggcgccg gtgacgaggg cgatgcgcgg gtggtggagc
     2581 atgggcaggc ctgttcggga ggtgtcgagc gcggaccgca tgggcccgcg tgccggcagg
     2641 gagtttaccg ggcgttcgcc gtgttcgttt tccggttgcg gaggaagggc agcaagacca
     2701 gcagcccgac cgcgtcggcg agccagtcga gcggatcgcc gacgcggtag gcggtggtcg
     2761 actgcgccac ctccatcgcc gcgccgtagg ccagcagccc ggccgcgagc aggccgacgc
     2821 gttgcggcca ggcggcgacg ccgaggatcg cgaggccggc gaagagcagg gcgtgctcga
     2881 ccttgtcctg ccaggagaac agcttgatca ccggctcgat cggcatcagc gcgagccaga
     2941 aggcgccacc gagggcggcg atgaagagca cgcgcagcag cgtgcgaacg gatgtgggca
     3001 tctgtggtcc gaaaaagcgg ttggctgcct gtcggtggtg cggcgcgcac agcagcgctg
     3061 ggaaggtggc ggcggttcgg tgtcgtgcgt cgatggctgg cggagcttag cggcaaatgc
     3121 gcggcgcgtg gtgcggcgcg caataacctg gggagatgac ccgatgcagg atggcaaagt
     3181 tcgcgaactt tcgagctcgc gcacgcgcgc agcgctgcgc gtcgcgctgc tcgccggcat
     3241 gctcgccgcc ggcagcatgc acgccccgct cgcgggcgcg accggcatgg tgtatccgag
     3301 cgagacgggg ccgctgcgcg tgaccgaggt cgcgcgcggt ttggagagtc cgtgggcgct
     3361 cgccttcctc cccgacggtc gcatgctggt gaccgagcgc ccggggcgga tgcgcatcgt
     3421 cgatacggca gggcgggtgt cggaacccat cgccggcgtg cccgaggtcc atgcgcgcgg
     3481 gcagggcggc ctgctcgacg ttgcgctcgc gcccgacttc gaccgtgacc gcctgatcgt
     3541 gttctcctac gccgagccga ccgcgcgggg agcgcgcacc gccgtggcgc gcgcgcggct
     3601 cgacgtcgac ggcctgcgcc tcgaggacgt gcgccgcatc ttcgcgcagg acgaggatcc
     3661 cgcgggcaat caccactggg gctcgcgcct ggtgttcggg cgcgacggcc gcctgttcgt
     3721 gaccctgggc gatcggttcc accaccgcga gcgcgcgcag gcgctcgaca gccatctcgg
     3781 caaggtggtg cgcatcgatc ccgacggcgg tgtgcccgcc gacaacccgc tcgtggggcg
     3841 tgcaggcgtg cgcggcgaga tctggtcctg gggccaccgc aacgtgcagg gtgcggcctt
     3901 gcacccgcgc accggcgagc tgtggaccca cgagcacggc ccgcagggcg gcgacgagat
     3961 caaccgcacc ctgggcgggc gcaattacgg ctggccggag atcacctacg gccgcgaata
     4021 cgtcaccggt cgcaagatcg gcgccggcag cgagcgcgaa gacgtggcgg cgccggtgct
     4081 gcaatggaca ccctcgatcg ccccctcggg catggccttc tataccggcg atgccttccc
     4141 gcagtggcag ggcaacctct tcgtcggcgc gctcaagttc cagctgctcg cccgcctggt
     4201 gctcgatggc gagcgcgtgg tgcgcgagga gcgcctgctc gaaggcctgg ggcggatccg
     4261 cgacgtgcgc caggggccgg acggccgcct gtggctgctc gacgagagcg ccgggcgggt
     4321 gctgcggatc gatccgcagt gagcggggct cgcgtgctcg tccccggaag cctcgcgggg
     4381 gcgattcacc ttagcccgcc tgcttgatcg ccagcaccgc ctggttgcgc agggtgcgca
     4441 gcatggcgag ctcctgctcg ccgatgcgca gctctccggc gcgatccttg tcggcgtaga
     4501 tcatcgccac cgggcggccc ttgatcgtga gtggaaagag cacgaaggtg cgcgccgcca
     4561 ccgacctgcg gtaccacgcc ggcacgcggg cggcgatctt ggggtcgtcc acgtcgctga
     4621 tcagcacgtc cacgccgcgt gcggtggcca cgttgaagac gttgtcgggc tgggccgaga
     4681 ggctgaagcg cagctgcttg gcgagctcgc ctacctcggg gccgaagccg aagcgcccgc
     4741 acatggcgtt gctgcgcgcg tcgcgca
//
LOCUS       DAOQNN010000253         4741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11046_length_4741_cov_8.765898, whole genome shotgun sequence.
ACCESSION   DAOQNN010000253 DAOQNN010000000
VERSION     DAOQNN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11046_length_4741_cov_8.765898"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="PKV97_12300"
     CDS             complement(<1..1482)
                     /locus_tag="PKV97_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06706.1"
                     /translation="MNPGAPAPADDAFDAVFAIRLPPRPYPGLRPFEQHEWPIFFGRE
                     RMTDEIVDRLLGHRLLVVHGDSGCGKSSLVRAGVLPRLEQESARGGVRWRTCTTLPRA
                     APLWNLARALAALDTPGGDGHGLELRTIAFRRALNFGREAPAALAELLGCGPRSQVCI
                     LIDQFEELFEHARRHGAEEATLLAACLVGLLDAPPAGLYAVLTMRSEFLGACSRYEGF
                     AEAVNRTQYLLPRMEHDDLMRAIREPAVLYDGEVTRELAERLIVDGGGGQDQLPLIQH
                     GLMLLHDERARAAGLALAGPTPGAPAWRLGVEHYHAEHGLAGLLSAHADAVQARAEQQ
                     CLGGEATRVVEDLFRALTDINAEGQAIRRPCPLARLVAVTGAEESVLRCVVDTFRADG
                     VSFLEPYGHEALPADELIDISHEALIRCWRRIAEPREGWLAREFRNGLVWRALLVQAD
                     SFERDPGNVLGATTTDERERWLRRRNAAWAERYGGGWERVQRLL"
     gene            complement(1479..2579)
                     /locus_tag="PKV97_12305"
     CDS             complement(1479..2579)
                     /locus_tag="PKV97_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNB06707.1"
                     /translation="MSYVGEPFRHDLFVSYSHGDDGSGGACLAPWSAAFAHELERELR
                     ADRRFRTSLSVFRDQDHRPAQSLDPMAPLTAQLQEQIAGAALLVVLMSPDYLASKWCA
                     DERDWWLQRQAALGLPHEERIAVVRIWPTDTPWPAALCDARGEPLVGFAFYPADGVPP
                     RPLGWTDLPGAFGSEFRKALLDMVGRLYPKLEAMRARTEELRRQREEAERLVRDGGQL
                     LYLHGRSDHAAAWEEAALKLVDKGFAVVPGEPDRVDSDPLRLQAVRERRVETLSGCDA
                     LLLLGTEDGRALDADLVVVGKHDRQSARARSHRLLPCGLLDTAGATIATNVRRATARI
                     VQADWIDGTDAGWPVAVRDWLGGKGLQAGGAP"
     gene            complement(2718..3845)
                     /locus_tag="PKV97_12310"
     CDS             complement(2718..3845)
                     /locus_tag="PKV97_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HNB06708.1"
                     /translation="MSKRLHSLSSLLRAAVSLAAAALAAIALAAPPGALPALNIAIED
                     TSVSGISSGGFMSVQMQVAHSGIVRGAGVVAGGPYYCAQDSVLKATTECSCTGAPTLS
                     CKVGEASTAVPELVAATRAFRAEGRIDDPAQIARQRVVTVVGGKDALVPAAITRQLHG
                     YYDAFGLPAAALKPVTLDAAGHTMPTTAYGGACGTTDEPYIGSCGFEAAHAILEWIYG
                     PDVAPPRTTPTPAGRFVEFDQRAYIPATGFFSHLWGTGLDKTGWVYIPPACAAGERCR
                     LHVVFHGCKQGQSFLPLRRPPDGGLYNGTTFVRNTGYDRWADTNRLVVLFPQAVSIPW
                     KNPNGCWDWWGYTDAHYATRDGIQIRSVRAMIERLASGRHD"
     gene            4055..>4741
                     /gene="menA"
                     /locus_tag="PKV97_12315"
     CDS             4055..>4741
                     /gene="menA"
                     /locus_tag="PKV97_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HNB06709.1"
                     /translation="MTPPHPAPSRPGTLAVWWAAIRPRTLGMALAPVMLGTALAVADG
                     AELAWAVLFATVSCALLIQIGTNLYNDAIDFERGTDRPERLGPLRVTLAGWASAAEVK
                     RGALLCLLLALLLGAWLCVVGGALILAIGLASLIAGWAYSGGPRPISHSPLGELFVLL
                     FFGVFAVLGSHWLQSRALGHLDLLAGLAVGAPAAAVLLVNNYRDLAGDLRAGRRTLAA
                     LLGAEGARRAF"
BASE COUNT          583 a         1731 c         1784 g          643 t
ORIGIN      
        1 gagcaggcgc tgcacgcgct cccagccgcc accgtagcgt tccgcccagg cggcgttgcg
       61 ccggcgcagc cagcgttcgc gctcgtcggt ggtggtggcg ccgagcacgt tgcccgggtc
      121 gcgctcgaag ctgtcggcct gcaccagcag cgcgcgccac accaggccgt tgcggaactc
      181 gcgcgccagc cagccctcgc gcggctcggc gatgcgccgc cagcagcgga tcagggcctc
      241 atggctgatg tcgatgagct cgtcggcggg gagcgcctcg tgcccgtagg gctcgaggaa
      301 ggacacgccg tcggcacgga aagtgtccac cacgcagcgc agtacggact cctcggcgcc
      361 ggtcaccgcc accaggcgtg cgagcgggca gggccggcgg atggcctggc cctcggcgtt
      421 gatgtcggtg agcgcgcgga agaggtcttc caccacccgc gtcgcctcgc cgccgaggca
      481 ttgctgctcg gcgcgcgcct gcacggcgtc ggcgtgtgcc gagagcaggc cggcgaggcc
      541 gtgctcggcg tggtaatgct ccaccccgag ccgccacgcc ggcgcgcccg gggtcggtcc
      601 ggcaagggcc aggcccgcgg cacgcgcacg ctcgtcgtgg agcagcatca gcccgtgctg
      661 gatcagcggc agctggtcct gtccgccgcc gccgtccacg atcaggcgct cggcgagctc
      721 gcgcgtcacc tcgccgtcgt agagcaccgc cggctcgcgg atcgcccgca tgaggtcgtc
      781 gtgctccatg cgcggcagca ggtattgcgt gcgattgacc gcctcggcga agccctcgta
      841 gcgcgagcat gcgccgagaa actccgagcg catggtcagc accgcgtaca gcccggccgg
      901 cggcgcatcg agcaggccga ccaggcaggc ggccagcagg gtcgcttctt ccgcaccatg
      961 gcggcgggcg tgctcgaaga gctcctcgaa ctggtcgatg aggatgcaga cctggctgcg
     1021 cgggccgcag ccgagcagct cggcgagcgc cgccggggct tcgcggccga agttgagcgc
     1081 gcggcggaag gcgatcgtgc gcagctccag cccgtgcccg tcgccgccgg gcgtgtcgag
     1141 cgcggcgagc gcacgcgcga ggttccacag cggcgcagcg cgcggcagtg tggtgcaggt
     1201 acgccagcgc acgccgccgc gcgcgctctc ctgctccagc cgcggcagca cgccggcgcg
     1261 caccagcgag ctcttgccgc agcccgagtc gccatggacc acgagcaggc gatggccgag
     1321 caggcggtcg acgatctcgt cggtcatgcg ctcgcggccg aagaagatcg gccactcatg
     1381 ttgctcgaag gggcgcaggc cgggataggg gcggggcggc aggcggatcg cgaagaccgc
     1441 gtcgaaggcg tcgtcggcgg gggcaggcgc gccggggttc atggtgcgcc cccggcctgc
     1501 aagcccttgc cgcccagcca gtcgcgcacc gcgaccggcc agcctgcgtc ggtgccgtcg
     1561 atccagtccg cctgcacgat gcgcgcggtg gcgcggcgca cgttggtggc gatggtggcg
     1621 ccggcggtgt cgagcaggcc gcagggcagc agtcggtgag agcgtgcgcg cgccgactgg
     1681 cgatcgtgct tgcccaccac cacgaggtcg gcatcgaggg cgcgcccgtc ctcggtgccg
     1741 agcagcagca gggcgtcgca gccgctcagg gtctccaccc tgcgctcgcg cacggcctgc
     1801 aggcgcagcg gatcgctgtc gacgcggtcg ggctcgcccg gcaccaccgc gaagcccttg
     1861 tcgaccagct tgagcgccgc ctcttcccaa gccgcggcgt ggtcgctgcg gccgtgcagg
     1921 tagagcagct ggccgccgtc gcggaccagg cgctcggcct cctcgcgctg gcggcgcagc
     1981 tcctcggtgc gcgcgcgcat cgcctcgagc ttggggtaga gccggccgac catgtcgagc
     2041 agggccttgc ggaactcgct gccgaaggcg cccggcaggt cggtccagcc gagcgggcgc
     2101 ggcggcacgc cgtcggccgg gtagaaggcg aagccgacca gcggctcgcc gcgcgcatcg
     2161 cacaacgccg ccggccacgg tgtgtcggtg ggccagatgc gcaccaccgc gatacgctcc
     2221 tcgtgcggca ggccgagcgc ggcctggcgc tgcaaccacc agtcgcgctc gtcggcgcac
     2281 cacttcgacg cgaggtagtc gggcgacatc agcaccacca gcagcgccgc gccggcgatc
     2341 tgctcctgca gctgggcggt gagcggtgcc atcgggtcga ggctctgcgc cgggcggtgg
     2401 tcctggtcgc ggaacacgct caggctggtg cggaagcggc ggtcggcacg cagctcgcgc
     2461 tcgagctcgt gcgcgaacgc ggccgaccac ggcgcgaggc aggcgccgcc gctaccgtcg
     2521 tcgccgtggc tgtagctcac gaagaggtcg tggcggaagg gctctccgac ataggacatg
     2581 gggactccct cgcgcgggcc cgtgcatcgc cgcagcccgt cccgcgtggg tgggccgccg
     2641 ggcggcggcc cctgctttcc ttatagtcca cgcgccgggg ctgggccaca cgggctgcgc
     2701 ccgcgccacc gcggcgctca gtcgtggcga cccgaggcca gccgctcgat catcgcgcgc
     2761 acgctgcgga tctggatgcc gtcgcgggtg gcgtagtgcg cgtcggtgta gccccaccag
     2821 tcccagcaac cgttggggtt cttccacggg atcgacaccg cctgcgggaa cagcaccacc
     2881 aggcggttgg tgtcggccca gcggtcgtag cccgtgttgc gcacgaaggt ggtgccgttg
     2941 tacagcccgc catcgggcgg ccgccgcaag ggcaggaagc tctgcccctg cttgcagccg
     3001 tggaacacca cgtgcagccg acagcgctcg cccgccgcgc aggcgggcgg gatgtagacc
     3061 cagccggtct tgtccaggcc ggtgccccac agatggctga agaagccggt cgcggggatg
     3121 taggcgcgct ggtcgaactc gacgaagcgg cccgccggcg tgggcgtggt gcgcggcgga
     3181 gcgacgtcgg ggccgtagat ccactcgagg atcgcgtgcg cggcctcgaa gccgcagctg
     3241 ccgatgtagg gctcgtcggt cgtgccgcag gcgccgccgt aggcggtggt gggcatggtg
     3301 tggccggcgg cgtcgagcgt caccggcttg agtgcggccg cgggcaggcc gaaggcgtcg
     3361 tagtagccgt gcagctggcg ggtgatcgcg gccggcacca gggcatcctt tccgcccacc
     3421 acggtcacca cgcgctggcg ggcgatctgc gccgggtcgt cgatgcggcc ctcggcccgg
     3481 aaggcgcgcg tggccgcgac cagctcgggc accgccgtgc tggcctcgcc gaccttgcac
     3541 gacagcgtcg gcgcgccggt gcaggagcat tcggtcgtgg ccttgagcac gctgtcctgc
     3601 gcacagtagt agggcccgcc ggcgaccacc cccgcgccgc ggacgatccc ggagtgggcg
     3661 acctgcatct gcaccgacat gaagccgccc gaagagatgc ccgacaccga ggtgtcctcg
     3721 atggcgatgt tgagcgccgg cagcgctccg gggggcgcgg cgagggcgat ggcggcgagg
     3781 gcggccgcgg cgaggctgac cgcggcgcgg aggagggagg aaagagagtg caggcgcttg
     3841 ctcatggcgg acctccggca ggcggcgtgg gggtggattc gtgtccagca tagtcgagcg
     3901 cggcgcatcc gcgggagctg cgcggcggct atgcgataat ccgcggtctt cccgcttgtc
     3961 ccgccatgtc cgtttccctg cccttcgccc cgcacgccct tccgtcctca cggctcgccc
     4021 ccatcggcgc cgctcccgcc cgtcgaggcg gggcgtgacg ccgccgcatc ccgcgccatc
     4081 ccgcccgggc acgctcgcgg tttggtgggc ggcgatcagg ccgcgcaccc tgggcatggc
     4141 gctcgccccg gtcatgctcg gcaccgcgct cgccgtcgcc gacggcgccg agctggcgtg
     4201 ggcggtgctg ttcgccaccg tgtcctgcgc gctgctgatc cagatcggca ccaacctcta
     4261 caacgacgcg atcgacttcg agcgcggcac cgatcgcccc gagcgcctcg ggccgctgcg
     4321 ggtcaccctc gccggctggg cgagcgccgc cgaggtcaag cgcggcgccc tgctctgcct
     4381 gctgctcgcg ctcctgctcg gcgcctggct ctgtgtcgtc ggcggcgccc tgatcctcgc
     4441 gatcgggctg gcctcgctga tcgccggctg ggcctattcc ggcgggccac ggccgatctc
     4501 gcactcgccg ctcggcgagc tcttcgtgct gctcttcttc ggcgtgttcg cggtgctcgg
     4561 cagccactgg ctgcagtcgc gcgcgctcgg ccacctcgac ctgctcgccg ggctggcggt
     4621 gggtgcgccc gccgccgcgg tgctgctggt caacaactat cgcgacctcg ccggcgacct
     4681 gcgcgccggg cgtcgcaccc tcgccgcgct gctcggcgcg gagggcgcgc gccgcgcctt
     4741 c
//
LOCUS       DAOQNN010000254         4740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11050_length_4740_cov_11.023693, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000254 DAOQNN010000000
VERSION     DAOQNN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11050_length_4740_cov_11.023693"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..634)
                     /locus_tag="PKV97_12320"
     CDS             complement(149..634)
                     /locus_tag="PKV97_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNB06710.1"
                     /translation="MPSFDILSEVDQPALRNAVEQANRKVDGRHDFKGTSAKIELAEK
                     LLTLYGDSDFQLEQIKTILLPEMTKKNVDVRCLDYGDVQKISGNKVKQEVKVRVGVEQ
                     ELAKKIVKLLKDSKMKVQAAIQGDAVRVSGAKRDVLQEAIALVKKQITDFPLQYGNFR
                     D"
     gene            complement(650..973)
                     /locus_tag="PKV97_12325"
     CDS             complement(650..973)
                     /locus_tag="PKV97_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNB06711.1"
                     /translation="MGITAKLDGVAVTTQANVYFDGKCVSHSVQFADGTKKSVGVILP
                     ASLTFNTGAPEIMEGVAGSCRVRLKGQADWKTYGAGESFDVPGDSSFDIEVAGEPYHY
                     ICHFG"
     gene            complement(988..1218)
                     /locus_tag="PKV97_12330"
     CDS             complement(988..1218)
                     /locus_tag="PKV97_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HNB06712.1"
                     /translation="MANDDYILGLSFVEFEDLALKLGLTGLILFMVFIIWNLGKESKA
                     GRYGMMWLFLGLGVGFVGFIAKGIIQKLLGIE"
     gene            complement(1240..2469)
                     /locus_tag="PKV97_12335"
     CDS             complement(1240..2469)
                     /locus_tag="PKV97_12335"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNB06713.1"
                     /translation="MSDVNKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEE
                     LEPARAKALQFGIQPENIFIDDLREEFVRDFVFPMFRANTIYEGEYLLGTSIARPLIA
                     KRQIEIARATGADAVSHGATGKGNDQVRFELGYYALMPGVKVIAPWREWDLLSREKLL
                     AYAEKHGIPIEMKHKQGGSPYSMDANLLHISFEGRHLENPAAEAEESMWRWTVSPEAA
                     PDAAEYIDLEFEQGDLVAIDGKRMKAHELLARLNELGGKHGIGRLDLVENRYVGMKSR
                     GCYETPGGTILLRAHRAIESITLDREVAHLKDDLMPRYASMIYNGYWWSPERRAIQAL
                     IDHTQQTVNGWVRLKLYKGNVIVTGRDSKTDSLFDPTIATFEDDAGAYNQKDAHGFIR
                     LNALRMRIAANAKAKRG"
     gene            complement(2537..3463)
                     /gene="argF"
                     /locus_tag="PKV97_12340"
     CDS             complement(2537..3463)
                     /gene="argF"
                     /locus_tag="PKV97_12340"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNB06714.1"
                     /translation="MSSPIKHYLQFKDFTREEYEYVFARAKWIKDKFKRYEPYHPLFD
                     RTLVMIFEKASTRTRLSFEAGMHQLGGSAIYLNTRDSQLGRGEPVEDAGQVISRMSDL
                     VMIRTFEQDIVERFAANSRVPIINGLTNEYHPCQILADILTFIEHRGSIQGKTVAWIG
                     DSNNMCNTWLQAAEVLDFNVHVSTPPGYEVEPERAGLYGTANFEQFADPMDACRGADL
                     VTTDVWTSMGFEAENEERMKAFADWCVDAEMMSVAHKEAVFMHCLPAHRGEEVTAEVI
                     DGPQSVVWDEAENRLHAQKALMEYLVLGRIEN"
     gene            complement(3473..4648)
                     /locus_tag="PKV97_12345"
     CDS             complement(3473..4648)
                     /locus_tag="PKV97_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNB06715.1"
                     /translation="MSHVMNTYARLPVAFTHGEGVWLYDETGKRYLDALSGIAVNTLG
                     YKHPRLVKAIADQAERVLHTSNLYRIPLQEQLSDRIAEAAGMDEVFFCNSGCEANEAA
                     IKLARMYGHNKGIELPHIIVMENAFHGRTMATLSATGNRKAQAGFEPLVQGFIRVPYK
                     DIEAIRRIAEHNHTVVAVMLEMIQGEGGVNVADEAFQRDLRALCDDRGWLMICDEVQC
                     GMGRTGKWFGWQHAGTRPDVMTLAKGLASGVPIGACVTSGLAKGLFGPGNHGSTFGGN
                     PLACAAGLATFDAIVEDELMDNAVAVGAAIRKGMAEALAGEAGVVDIRGRGLMIGIEL
                     DRPCGVLMARAAENGLLLSVTSERVVRLLPALTFTTADAQTLVSMLAPMIRDFLANG"
BASE COUNT          717 a         1509 c         1613 g          901 t
ORIGIN      
        1 cgctgcttct cgtgggagcg acgcaagtcg cgattccccg cccggaacat cgcctcgtcg
       61 ccgtcaaccc gactccccca caacccaccc cccgaacgca cgacgggcgc ctcgcggcgc
      121 ccgtcggatc cctgccccgg cgttccgctc agtcgcggaa gttgccgtac tgcagcggaa
      181 aatcggtgat ctgcttcttg accagcgcga tcgcctcctg caatacgtcg cgcttggcgc
      241 cggagacgcg caccgcgtcg ccctggatcg cagcctgcac cttcatcttg ctgtccttca
      301 agagcttgac gatcttcttg gccagttcct gctccacgcc gacccgcacc ttgacctcct
      361 gcttgacctt gttgccggag atcttctgca cgtcgccgta gtcgagacat cggacgtcga
      421 cgttcttctt ggtcatctcg ggcagcagga tggtcttgat ctgctcgagc tggaagtcgc
      481 tgtcgccgta gagggtcagc agcttctcgg cgagctcgat cttggcgctc gtgcccttga
      541 agtcgtgacg gccgtcgacc ttgcggttgg cctgctcgac cgcgttgcgc agcgcgggct
      601 ggtcgacctc ggacaggatg tcgaaagacg gcatgctccc ctcccccgct cagccgaagt
      661 ggcagatgta gtggtagggc tcgcccgcca cctcgatgtc gaagctggag tcgcccggca
      721 cgtcgaagga ttcgccggcg ccgtaggtct tccagtccgc ctggcctttc aggcggacgc
      781 ggcaggagcc ggccacgccc tccatgatct cgggcgcgcc ggtgttgaag gtgagcgagg
      841 ccggcaggat gacccccacg gatttcttcg tgccgtccgc gaactgcacg ctatggctga
      901 cgcacttgcc gtcgaaatag acgttggcct gggtggtgac ggcgacgccg tcgagcttgg
      961 cggtaatgcc catcgcttgt gttttcctta ttcgatgccg agcagcttct ggatgatgcc
     1021 cttggcgatg aagccgacga agcccacgcc caggcctagg aacagccaca tcatgccgta
     1081 gcggccggcc ttggattcct tgcccaggtt ccagatgatg aagaccatga acaggatcag
     1141 gccggtcaag ccgagtttga gggcgaggtc ctcgaactcg acgaagctga gtccgaggat
     1201 gtagtcgtcg ttggccatcg caagggatcc gttgcgggtt tagccgcgct tggccttggc
     1261 gttggccgcg atgcgcatgc gcagcgcgtt caggcggatg aagccgtgcg cgtccttctg
     1321 gttgtaggcg ccggcgtcgt cctcgaaggt ggcgatcgtc gggtcgaaca gcgagtcggt
     1381 cttcgagtcg cgaccggtga cgatgacgtt gcccttgtag agcttgaggc gtacccagcc
     1441 gttgacggtc tgctgggtgt ggtcgatgag cgcctggatc gcgcggcgct cggggctcca
     1501 ccagtaaccg ttgtagatca tgctggcgta gcgcggcatg aggtcgtcct tgaggtgggc
     1561 gacttcgcgg tcgagcgtga tcgattcgat ggcgcggtgg gcgcgcagca ggatggtgcc
     1621 ccccggggtc tcgtagcagc cgcggctctt catgccgacg tagcggttct ccaccaggtc
     1681 gaggcggccg atgccgtgct tgccgccgag ctcgttgagc ctggcgagca gctcgtgcgc
     1741 cttcatgcgc ttgccgtcga tcgcgaccag atcgccctgt tcgaactcga ggtcgatgta
     1801 ctcggcggcg tcgggcgcgg cctccggcga caccgtccag cgccacatcg actcctcggc
     1861 ctcggcggcg gggttctcga ggtggcggcc ctcgaaggag atgtgcagca ggttggcgtc
     1921 catcgagtag ggcgagccgc cctgcttgtg cttcatctcg atggggatgc cgtgcttctc
     1981 tgcgtaggcg agcagctttt cgcgcgacag caggtcccac tcgcgccacg gcgcgatgac
     2041 cttcacgccc ggcatcagcg cgtagtagcc gagctcgaag cggacctggt cgttgccctt
     2101 gccggtggcg ccgtgcgaga cggcatcggc gccggtggcg cgcgcgatct cgatctggcg
     2161 cttggcgatc agcggacgcg cgatcgaggt gccgagcagg tattcgcctt cgtagatggt
     2221 gttggcgcgg aacatcggga agacgaagtc gcgcacgaac tcctcgcgca ggtcgtcgat
     2281 gaagatgttc tcgggctgga taccgaactg cagcgccttg gcgcgcgcgg gctcgagctc
     2341 ttcaccctgg ccgaggtcgg cggtgaaggt gaccacttcg cactggtagg tgtcctgcag
     2401 ccacttcagg atgaccgaag tgtccagccc gcccgagtag gcgagcacta ctttgttgac
     2461 gtcgctcatg ctgttttcct gtttcctgct gtgttgccgg cgcgcacgcc cgtgcgtgcc
     2521 gtgattgttc gttggatcag ttctcgatgc ggccgaggac caggtattcc atcaacgcct
     2581 tctgcgcatg cagtcggttc tcggcctcgt cccacaccac ggactgcggg ccgtcgatga
     2641 cttcggcggt gacttcctcg ccgcggtgcg cgggcaggca gtgcatgaag acggcctcct
     2701 tgtgcgcgac cgacatcatc tcggcatcga cgcaccagtc ggcgaaggcc ttcatgcgct
     2761 cttcgttttc ggcctcgaag cccatcgagg tccacacgtc ggtggtgacc aggtcggcgc
     2821 cgcggcaggc gtccatcggg tcggcgaact gctcgaagtt ggccgtcccg tacaggccgg
     2881 cgcgctcggg ctcgacttca tagcccggcg gggtcgacac gtgaacgttg aagtccagca
     2941 cttcggcggc ctgcagccag gtgttgcaca tgttgttgct gtcgccgatc caggccacgg
     3001 tcttgccctg gatcgagccg cggtgctcga tgaaggtgag gatgtcggcg aggatctggc
     3061 aggggtggta ctcgttggtg aggccgttga tgatcggcac gcgcgagttg gcggcgaagc
     3121 gctcgacgat gtcctgctcg aaggtacgga tcatcaccag gtcgctcatg cgcgagatca
     3181 cctggccggc gtcctccacc ggctcgccgc ggccgagctg cgagtcgcgg gtgttgaggt
     3241 agatcgccga gccgccgagc tggtgcatgc cggcctcgaa cgagaggcgc gtgcgcgtgc
     3301 tcgccttctc gaagatcatc accagcgtgc ggtcgaagag cgggtggtag ggctcgtagc
     3361 gcttgaactt gtccttgatc cacttcgcgc gcgcgaacac gtattcgtat tcctcgcggg
     3421 tgaaatcctt gaactggagg tagtgcttga tgggagagct catacgcagt cctcagccgt
     3481 tcgcgaggaa gtcgcgaatc atcggcgcca gcatggacac cagggtctgc gcgtcggcgg
     3541 tggtgaaggt gagcgccggc agcaagcgca ccacgcgctc ggaggtcacc gacagcagca
     3601 ggccgttctc ggcggcgcgc gccatcagca cgccgcaggg gcggtcgagt tcgatgccga
     3661 tcatgaggcc gcgaccgcgg atgtcgacca cgcccgcctc gccggcgagc gcctcggcca
     3721 tgcccttgcg gatcgccgcg ccgacggcga cggcgttgtc catgagctcg tcctcgacga
     3781 tggcgtcgaa ggtggccagg cccgccgcgc aggcgagcgg gttgccgccg aaggtggagc
     3841 catggttgcc cggcccgaac aggcccttgg ccaggccgct ggtcacgcag gcgccgatcg
     3901 gcacgcccga ggccagaccc ttggccagcg tcatcacgtc gggcctcgtg cccgcatgct
     3961 gccagccgaa ccacttgccg gtgcggccca tgccgcactg gacctcgtcg cagatcatca
     4021 gccagcccct gtcgtcgcac agggcgcgca gatcacgctg gaaggcctcg tcggcgacgt
     4081 tgaccccgcc ctcgccctgg atcatctcga gcatcaccgc gaccacggtg tggttgtgct
     4141 cggcgatcct gcggatggcc tcgatgtcct tgtagggcac gcggatgaag ccctgcacca
     4201 gcggctcgaa accggcctgg gccttgcggt tgccggtggc cgacaacgtg gccatggtgc
     4261 ggccgtggaa ggcgttctcc atgacgatga tgtgcggcag ctcgatgccc ttgttgtggc
     4321 cgtacatgcg cgcgagcttg atcgccgcct cgttggcctc gcagccggag ttgcagaaga
     4381 acacctcgtc catgcccgcg gcttcggcga tgcggtcgga gagctgctcc tgcagcggga
     4441 tgcgatacag gttggaggtg tgcaggacgc gctcggcctg gtcggcgatg gccttgacca
     4501 ggcgggggtg cttgtacccg agcgtgttga ccgcgatgcc ggagagggca tcgagatatc
     4561 gcttgcctgt ctcgtcgtac agccacacgc cctcgccgtg cgtgaaggcc acgggcaggc
     4621 gggcgtaggt attcatgaca tgggacatgg gggtacctcg atcggtgcga agccggaaaa
     4681 cgcacacggc ggcagacgcc gccgtgcagg aaatgtgccg atgttaagcc aaaacggccg
//
LOCUS       DAOQNN010000255         4728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11100_length_4728_cov_9.025893, whole genome shotgun sequence.
ACCESSION   DAOQNN010000255 DAOQNN010000000
VERSION     DAOQNN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11100_length_4728_cov_9.025893"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..1456)
                     /locus_tag="PKV97_12350"
     CDS             complement(272..1456)
                     /locus_tag="PKV97_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HNB06716.1"
                     /translation="MGLKIYLSSTFEDLKDYRRRVYDQLRTLRHDVIAMEDYVAADER
                     PLDKCLRDVRDSDVYIGLFAWRYGYVPRAGNPQRRSVTELEYLEAKKHKKPCLIFLTD
                     NRAPWPPDQMDSTTGDNDSGKKIAALRKALREKGLVSTFETPEALAIKVVTALYSWQS
                     ETSRAQSQLGHAAVEEALAPGVQAAARRTNPLMWVPGSRLRVRFLGADGAFAARILRL
                     AQIWSAYANIFFVASEDKDAEVRVAFDKDGGSWSYEGTNALAVPPSEPTMNLGWLVPA
                     LPIDDVESVVLHEFGHVLGLAHEHNNPSGDIPWDRKEVLKLLGGPPNHWDQNTIDQYL
                     YRTWETDRFPFAKPFDPLSIMSYFFPKEATSGKQIFSTNTTLSSGDKEFISRLYPYAT
                     GG"
     gene            1660..2073
                     /locus_tag="PKV97_12355"
     CDS             1660..2073
                     /locus_tag="PKV97_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB06717.1"
                     /translation="MNTLGWQELKVVALAVDDLARAEDFYRSKLGLEPRPQADGDVGF
                     ALGGLTLLLKPLAGPGGAAEKSPYPRLTVAVADAPRMERELAARGVTISDAVQLYEDG
                     RYWVGAFLDSEGNKLWFCSEAGKPAPSVSGGRSSP"
     gene            complement(2123..2509)
                     /locus_tag="PKV97_12360"
     CDS             complement(2123..2509)
                     /locus_tag="PKV97_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNB06718.1"
                     /translation="MPIAIWTPALSLGVDSLDADHQKLIDTLNMVFDAMLFDRSSAAL
                     HSALATLDDYVTGHFAREEAWMQARDHPDLARHRQEHEALRDQLALLCAMSDLSDQER
                     SVELLMVLRDWLLGHIAHSDRDASGP"
     gene            complement(2517..4166)
                     /locus_tag="PKV97_12365"
     CDS             complement(2517..4166)
                     /locus_tag="PKV97_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNB06719.1"
                     /translation="MMFPGIRRGSDSLMARRPDSLIHGLDDKPRALTLAALGLQHFMV
                     AAPNLAIVVMICRAAGVDAAAAEGVLSFSLIALAIATLVQCSRRLGSGYFIVSCNSGI
                     YLSASLAAATGGLPVVCGMTLVAGIFQAFFASLLVRARRFFPNEIVALSIIIVGIEIG
                     VIGLNRLSGAGVPGLVVGGATFFVSVVFGVFGRGRWRLYCSLLGMACGYLVYLTTGIP
                     GPSSASDAFSSALFGLPSSAHLGLEFDVGYLLPFLFAAVAASLKTMGAVDICARINDA
                     AWVRPEMHAIRRGVLVDGLSTTLSGLFGSTGTNSSTSSVGVSHASGATSRVIGIAVAA
                     WMLVFALSPAFTHLVVTLPDPVVGGMLVFTGCLVIANGFQILGTVPMDMRRAGVVAFP
                     LLVAIARVANAPLFAQLPDSVKPLMSSALAIGVFAALLVNAFLSWGNARQATVALDPG
                     HPVGERLRQVRRILDEWNVAHNVKVRVMLTLRELLDERASSLKLAFNGCTLHIGLTRA
                     APAQDAQTTPDGRFFADRVSDAMAHRKRANGSSVISLCFEQ"
BASE COUNT          781 a         1477 c         1704 g          766 t
ORIGIN      
        1 tcggtggccc agcgcttgac gttggcgtgc agctcgcatt gctgtatcgc gtttgccagc
       61 aggacctcgg cgtcgtgcgt cgacatgcct ccgccggaga gcaagttcgc cgcgtcgtgc
      121 agcgtcagtg tggtgccacg gtcgctcgtg ttggtcattt tgatctcccg ggccttgcag
      181 cgtgtgccga accgcgatct cgacaacggt gccggcggat ggtgcggcga gccagcgggg
      241 cgcgggatcg ggatctgcac ccgagttcca ttcagccccc cgtggcgtag ggatacagcc
      301 ggctgatgaa ctccttgtcg cccgatgaca gggtggtgtt ggtgctgaag atctgcttgc
      361 cgctggtggc ttccttgggg aagaagtagg acatgatcga cagcggatcg aagggtttgg
      421 caaaggggaa gcggtcggtc tcccaagtgc ggtagaggta ctggtcgatg gtgttctggt
      481 cccagtggtt gggcgggcca cccaggagct tgaggacctc cttcctgtcc cagggaatgt
      541 cgccgctggg gttgttgtgt tcgtgggcga ggcccagcac gtggccgaac tcgtgcagca
      601 cgaccgactc gacgtcgtcg atcggcagcg ccggcacaag ccagccgagg ttcatggtcg
      661 gctcgctcgg cggcacggcg agggcgttcg tgccctcgta ggaccaggag ccgccatcct
      721 tgtcgaaggc gacacgcacc tcggcatcct tgtcctcgct ggcgacgaag aagatgttgg
      781 cgtaggcgct ccagatctgc gcaagacgca ggatgcgtgc cgcgaaggcg ccgtcggcgc
      841 caaggaagcg caccctcagg cgcgagcccg gcacccacat caaggggttg gtcctgcgtg
      901 ccgccgcctg aaccccgggc gccagggcct cttcgaccgc agcatgccca agctgcgatt
      961 gggcgcgcga tgtctccgac tgccagctgt agagcgcggt gacgaccttg atcgcgagcg
     1021 cctccggcgt ctcgaaggtc gataccagcc ctttttcccg cagggccttg cgcaaggcgg
     1081 cgatcttctt gccgctgtcg ttgtcgccgg tggtggagtc catctggtcc ggcggccacg
     1141 gcgcgcgatt gtcggtcagg aagatcaggc agggcttctt gtgcttcttc gcctcgaggt
     1201 actccagctc ggtcaccgag cggcgctggg gattgccggc gcgcggcacg tagccgtagc
     1261 gccaggcgaa caggccgata tagacgtcgc tgtcgcgcac gtcgcgcagg cacttgtcga
     1321 gcgggcgctc gtcggcggcg acgtagtcct ccatcgcgat gacgtcgtgg cgcaaggtcc
     1381 gcaactggtc atacacgcgg cgccgatagt ccttcagatc ctcgaaggtc gaggataggt
     1441 agattttcag gcccatcgcc ttcccctcat gcggcccctg tgctttaaag gtagtgagcc
     1501 gcggcaaagt tgcaagatga acatcggctt ttcgggccgc ccgcattgac cgcttccggc
     1561 tggctttgcg ggcagtcgtc caccgcgtgg cccggcaaag cgccgcagcg taatcggggt
     1621 ctgtccccgt ttttcatcgg caagcggcga ggtgtggaga tgaacacgct gggatggcag
     1681 gaactgaagg tggtggcact cgccgtcgac gacctggcga gggcggagga tttttaccgc
     1741 agcaagctgg gcctcgagcc gaggccgcag gcggacggtg acgtcggttt cgccctgggc
     1801 ggcctgacgc tcctgctcaa gccgcttgcc ggacccggcg gcgcggcgga gaaatcgccc
     1861 tatccgcgcc tgaccgttgc ggttgcggac gcgccgcgga tggagcggga actggctgcg
     1921 cgtggcgtca cgatcagcga cgcggtgcaa ctctacgagg acggccgtta ctgggtcggc
     1981 gccttcctgg acagcgaggg caacaagctg tggttctgct ccgaagccgg gaagcccgcg
     2041 ccaagtgtct ctgggggcag gtcttcccct tgattgcaga ggaaacgcgg tcgtggggct
     2101 gccctcggct gtcccaggcc tttcatggcc cggacgcgtc gcggtccgag tgggcgatgt
     2161 ggccgagcag ccagtcgcgc agcaccatca ggagctcgac cgaacgctcc tggtccgaca
     2221 ggtcgctcat ggcgcacagg agcgcgagct gatcgcgcag cgcctcgtgt tcctgccgat
     2281 ggcgcgccag gtccgggtgg tcgcgtgcct gcatccacgc ctcctcgcgc gcgaagtgcc
     2341 cggtcacgta gtcgtcgagg gtggcgagcg cgctgtgcag ggccgcggag ctgcgatcga
     2401 acagcatcgc atcgaacacc atgttcagcg tgtcgattag cttctggtga tcggcgtcga
     2461 ggctgtcgac cccgagcgag agcgccgggg tccagatcgc gatgggcatg tcggcctcac
     2521 tgctcgaaac atagcgagat gacgctgctg ccgttcgcgc gcttgcggtg ggccatcgcg
     2581 tcgctgaccc ggtcggcgaa gaagcggccg tccggggtgg tttgcgcgtc ctgcgccggg
     2641 gccgcgcgcg tgagaccgat gtgcagcgtg cagccgttga aggcgagctt caggctggag
     2701 gcgcgctcgt cgagcagctc gcgcagggtg agcatgacgc gaaccttgac gttgtgggcc
     2761 acgttccatt cgtcgaggat cctccggacc tggcgcaggc gctcgccgac ggggtggccg
     2821 ggatcgaggg cgaccgtggc ttggcgggcg ttgccccagc tcaggaaggc gttgaccagc
     2881 agcgcggcga acacgccgat cgcgagcgcc gaggacatca gcggcttcac cgagtcgggc
     2941 agttgcgcga acaggggggc gttggcgacg cgtgcgatcg ccaccaggag cggaaacgcg
     3001 accacaccgg cccggcgcat gtccatcggg acggtgccga ggatctggaa tccgttggcg
     3061 atgaccaggc agccggtgaa gaccagcatc ccgccgacca cgggatccgg cagggtgacc
     3121 acgaggtgcg tgaacgccgg actgagtgcg aacaccagca tccatgcggc gacggcgatg
     3181 ccgatgacgc ggctggttgc gccgctcgcg tgcgaaaccc ccacgctcga ggtcgaggag
     3241 ttcgtgccgg tgctgccgaa caagcccgag agggtggtgg acagcccatc gacgagcacg
     3301 ccccgccgga tcgcgtgcat ctccggccga acccaggcgg cgtcgttgat ccgcgcacag
     3361 atgtcgacgg cacccatggt cttgagcgac gcggcgaccg cggcgaagag gaaggggagc
     3421 aagtagccga cgtcgaattc gagccccaga tgtgcggagg agggcaggcc gaacagcgcc
     3481 gacgagaagg cgtcgcttgc gctgctcggg ccggggatgc cggtcgtcag gtacacgaga
     3541 tagccgcagg ccatgccgag cagggaacaa tacaggcgcc agcggccgcg cccgaagacg
     3601 ccgaagacca ccgagacgaa gaaggtggcg ccgccgacca ccaggccggg gacgcccgcg
     3661 ccggagaggc ggttcaggcc gatgacgccg atctcgatgc cgacgatgat gatcgagagc
     3721 gcgacgatct cattcgggaa gaatcggcgg gcccgtacca gcaggctggc gaagaaggcc
     3781 tggaagatcc cggcgaccag cgtcatcccg cacacgaccg gcagcccgcc ggtggccgcg
     3841 gcgagcgagg ccgacagata gatccccgag ttgcagctga cgatgaaata ccccgagccg
     3901 agtcgtctgc tgcactggac cagcgtcgcg atggccagcg cgatgagcga aaacgagagc
     3961 accccctcgg cggcggccgc gtcgacgccc gcggcccggc agatcatgac gacgatcgcc
     4021 aggttgggcg ccgccaccat gaaatgctgg agcccgagtg cagcgagcgt cagggcgcgc
     4081 ggtttatcgt ccagtccgtg gatgaggctg tccggccgtc gcgccatcag ggagtcggac
     4141 ccgcggcgga taccgggaaa catcatcgtg attctccgag tccgcggata ggtggaacga
     4201 tggcggcgaa cgatccgggc gcgcccgacc tcgggccggc tgcaggtgtg gaacaccctt
     4261 caaccggccg gccgcgtcag ggtgggaatg gcgctcgggc gttgcgcgac aggcgcttcg
     4321 tcgcgggcga ttgattcaac atagacgagg aaacaggcgg ggtcaggcct ttcgttcgac
     4381 gctcaccgcg ctgtctgccg gcgctcgtgt gccgacgatg cgagccgcgt ctccactgca
     4441 tttcggcgcg ccagagtttg cgaggcaccg gttagcgccc aagatcgctg gatcgcctcg
     4501 gcaaggcaga tgatgatcgc cgcgcccctg tgcgtcgttt gtggccggtt ctagtgcgct
     4561 ggggggtatg gccgccgact ttccgtcgcg tgaaggccgg accggagcgc aggccgagtg
     4621 cgtgactacg cggtggccag attgcccgga tttggcggca ccatggctac atggcgccac
     4681 aaagcacaac gccagccccg ggaggctggc gcaagtgctt gaattcag
//
LOCUS       DAOQNN010000256         4663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11331_length_4663_cov_9.578776, whole genome shotgun sequence.
ACCESSION   DAOQNN010000256 DAOQNN010000000
VERSION     DAOQNN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11331_length_4663_cov_9.578776"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      277..501
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            770..1408
                     /locus_tag="PKV97_12370"
     CDS             770..1408
                     /locus_tag="PKV97_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNB06720.1"
                     /translation="MHIEPGILSGAKIAAANLAATALVAAQAPQLLRKPQRLLRTLLA
                     ALFFSVFMQSFHTPAGASELHFIGAMPIYLTLGFVPTLLGFGLGLLLQGGVFEPADLL
                     HLGVNFLSLAVPLVALHATLGRRLASGAATRVGSVLKLDAAYYAGVAVMVGFWLAMGE
                     VATPFAAWAAFASAYLPVALIEPVLTVALVRLLQSQAHRPLVRLCFAVPARG"
     gene            1408..2235
                     /gene="cobM"
                     /locus_tag="PKV97_12375"
     CDS             1408..2235
                     /gene="cobM"
                     /locus_tag="PKV97_12375"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HNB06721.1"
                     /translation="MTALPGTVWFVGAGPGDPDLITVKGRRLLERAGAILFAGSLVDR
                     SATQFAPAHCEIRDSRDMTLEGMSAWLLDACARHPVVVRLQTGDPGLYGALVEMTRPL
                     DAAGIPWRVVPGVSSAMAAAAAAGETLTLPEVTQTVILTRVAGRTPMPPGEALEALAA
                     HRTTLCIFLSITLLHEVQRALRAAGWPEDAPIVVVHKASWAGAEKIVRGTLADIKRRC
                     QDEKIASQAMILASPALGARLWPDLARSKLYDPGFGHRFRKASMPAAAAGIATGETA"
     gene            2232..>2986
                     /locus_tag="PKV97_12380"
     CDS             2232..>2986
                     /locus_tag="PKV97_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HNB06722.1"
                     /translation="MNKTAILLVGHGSRNREGNKEILHFAAQWRERHPGWRIETCFIE
                     HADVLLDEGLDRAAHAAHRVVTIPFILNAAGHVKMELPAAIERARARHPGVAFACARH
                     LGMGRELFAVLRGQLDRLLRQLDMPDPHTTGVVLLGRGSSDAGANGEFAKMARWLFED
                     GDHELVDLAFTGVTWPRLETVVQRQARLGMTQICIVPVYLFTGVLMERIRAQVERLRR
                     QYPQLAFALGTHFGFDEGIFALLDRRVDDHEAP"
     assembly_gap    2987..3086
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3116..3796
                     /locus_tag="PKV97_12385"
     CDS             3116..3796
                     /locus_tag="PKV97_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HNB06723.1"
                     /translation="MRTNTVTEQLTAAGRAIEHDSFAIIDAEVGRHAYTAGQWPVVRR
                     MIHANADFEFNGLTEFHPDAVDAGLAAILGGGARIVADVEMICVGLSASRLAHFGMRT
                     HQFISDPDVVEHARAEDSTRAVQAMRKAHRLGLLDGAIVGIGNAPTALIELVRLIRDE
                     GVRPALVVGMPVGFVSAAESKDRMAAVDAVPWIVIRGRKGGSTLVVAAIHALLGLAET
                     RQRQAVRG"
     gene            3806..>4663
                     /locus_tag="PKV97_12390"
     CDS             3806..>4663
                     /locus_tag="PKV97_12390"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HNB06724.1"
                     /translation="MAAGHSPPDKVRKGDARRARGNRTGLTTGANSAAAAAAATLGLV
                     RGAVPDAVECVLPNTTRVHFTIRDGQVDGDHAHAVSIKDAGDDPDATHGARLTADVRR
                     IRRGGGVVILAGGRGVGVVTKPGLGLAVGGPAINPVPRRNIIDNVRAAGAPILEAGDG
                     LEVTISVPGGEEMAQKTLNARLGILGGISILGTTGIVRPYSTAAFRASVIQAIDVAAN
                     QGQTCVVFTTGGRTEKCAMRAFPDLDEACFVQMGDFVKAAFTTAVRQGMRHIVVGAMI
                     GKLTKIAQGL"
BASE COUNT          633 a         1763 c         1513 g          654 t
ORIGIN      
        1 cggagggtaa cgcgccccgc cccgccggcg cgaacccgca gcgccgcccg cagccccgcg
       61 ccgccctgtt cgccgccaag ccgggcggcg acaagcgctg agcaggacga ccggaaacag
      121 cgaggccgtc ggcgcgggtc cgagattcgc ataacccgcg ccgatggccg catcgcgact
      181 ggtgtcgctc ccacgaaaac cggcatcgcg accgcggcgc tcccgtcccc accgctgcac
      241 atcgcttgcc ttcccgcccc agcttcgcct atccttcgct ccgcattcga tccgggtgcc
      301 ctgtcataac ttcatatgac agggtgaaac gggaagtccg gtgacgtcgt ccgccttgcg
      361 cgcgcacgac tattccggcg cagcccccgc tgctgtgagc ctgacgaatc cgccaacacg
      421 ccactgagcc ttgcgctcgg gaaggccggc ggacgaggaa gacggcaagc cagaagaccg
      481 gcccgatcga gtacggaacc cgcacccgcc cgcaagtggc gtcgtgcgcg gttccaggtt
      541 gatgtgagcc ccggggtgtg ggttcgatcc gactggtttt ccgccggctg cccgtgtgcg
      601 ccggaagctc cagccgactc gtcgtgcaag cggtctccag gcaatgccgt gcccggcgtg
      661 cgctcgcggt ttcaccgcgt gcgtgcgccc accggcagcg ccgcgcccgc atgccgcccc
      721 ccgcccgcgt ggttcgcatc gtcgttccat gcactcgtgg ggagtcatca tgcacatcga
      781 acccggcatc ctttccggcg ccaagatcgc cgccgccaac ctcgccgcca ccgcgctcgt
      841 cgccgcccag gcgccgcaac ttctcaggaa gccgcagcgc ttgctgcgca ccctgctcgc
      901 agccctgttc ttctccgtct tcatgcagag cttccatacg cccgccggcg catcggagct
      961 gcacttcatc ggcgccatgc cgatctacct gacgctcggc ttcgtgccca ccctgctcgg
     1021 cttcggcctc ggcctgctgc ttcagggcgg ggtcttcgag cccgccgacc tgctgcatct
     1081 cggggtcaat ttcctctcgc tggccgtacc gctggtggcg ctgcatgcca cgctcggccg
     1141 caggctcgcc agcggcgccg cgacgcgggt gggatcggtg ctgaagctcg acgccgccta
     1201 ctacgccggc gtcgccgtca tggtcggctt ctggctggcc atgggcgagg tcgccacgcc
     1261 cttcgcggca tgggccgcct tcgccagcgc ctacctcccg gtggcgctga tcgagccggt
     1321 gctgacggtc gcactcgtcc gcctgctgca gtcgcaggcg catcgtcccc tcgtgcggct
     1381 ctgcttcgcc gtgccggcgc gcggctgatg accgcgctgc cgggtacggt ctggttcgtc
     1441 ggcgcgggcc ccggcgaccc cgacctgatc accgtgaaag gccgccgcct gctcgagcgg
     1501 gcgggcgcga tcctgttcgc cggctcgctg gtcgaccgca gcgcgacgca gttcgcgccc
     1561 gcccactgcg agatccgcga ctccagggac atgacgctcg agggcatgag cgcctggctg
     1621 ctggacgcct gcgcccgcca tcccgtcgtg gtgcgcctgc agaccggcga ccccggcctg
     1681 tacggtgccc tggtcgagat gacgcgcccg ctcgacgccg ccggcattcc ttggcgggtg
     1741 gtgccgggcg tgtcctcggc gatggccgcg gccgccgccg cgggcgagac cctgaccctg
     1801 ccggaggtga cgcagacggt gatcctcacc cgtgtcgccg ggcgcacgcc gatgccgccg
     1861 ggcgaggcgc tcgaggcgct cgccgcgcac cgcacgacgc tgtgcatctt cctgtcgatc
     1921 acgctgctgc acgaggtgca gcgtgcgctg cgcgccgccg gctggccgga ggacgccccc
     1981 atcgtggtgg tgcacaaggc gagctgggcg ggcgcggaga agatcgtgcg cggcacgctg
     2041 gccgacatca agcgccgttg ccaggacgag aagatcgcca gccaggccat gattctcgcc
     2101 agtccggcgc tcggcgcccg cctgtggccc gacctcgcac gctcgaaact ctacgacccg
     2161 ggcttcggcc accgcttccg caaggcgtcg atgccggccg ccgcggccgg catcgccacc
     2221 ggagaaaccg catgaacaag accgccatcc ttctcgtcgg ccacggctcg cgcaaccgcg
     2281 agggcaacaa ggagatcctc cacttcgccg cacaatggcg cgaacgccac cccggctggc
     2341 gcatcgagac ctgcttcatc gagcacgccg acgtgctgct cgacgagggc ctcgaccgcg
     2401 ccgcgcacgc ggcgcaccgt gtcgtcacga ttcccttcat cctcaacgcc gcgggccacg
     2461 tcaagatgga gcttccggct gcgatcgagc gcgcccgcgc ccgccacccc ggcgtcgcct
     2521 tcgcctgcgc ccgccacctc ggcatggggc gcgagctctt cgccgtactg cgcggccagc
     2581 tcgaccgcct gctgaggcag ctcgacatgc ccgacccgca caccaccggc gtggtgctgc
     2641 tcggccgcgg ctcctcggac gccggcgcca acggcgagtt cgccaagatg gcgcgctggc
     2701 tcttcgagga cggcgaccac gagctggtcg acctggcttt caccggcgtg acctggccgc
     2761 gactcgagac cgtggtgcag cgccaggccc gcctgggcat gacgcagatc tgcatcgtgc
     2821 cggtgtacct cttcaccggc gtgctgatgg agcgcatccg ggcacaggtc gagcgcctgc
     2881 ggcgccagta cccgcagctc gccttcgcgc tcggcacgca cttcggcttc gacgagggca
     2941 tcttcgcgct gctcgaccgc agggtcgacg accacgaagc acccgannnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnccac gcgcacgcct gaagccgaac ccgccatgcg
     3121 caccaacacc gtcaccgaac agctcaccgc cgccgggcgc gcgatcgagc acgactcctt
     3181 cgccatcatc gacgccgagg tcggccgtca cgcctacacc gccgggcagt ggccggtggt
     3241 gcgccggatg atccacgcca acgccgactt cgagttcaac ggcctcaccg aattccatcc
     3301 cgatgcggtg gacgccggcc tggccgccat cctcggcggc ggcgcgcgga tcgtcgccga
     3361 cgtcgagatg atctgtgtcg gcctgtccgc ctcgcgtctc gcccacttcg gcatgcgcac
     3421 gcaccagttc atctccgacc ccgacgtcgt cgagcacgcc cgcgccgagg acagcacgcg
     3481 cgcggtgcag gccatgcgca aggcgcaccg cctcggcctg ctcgacggcg ccatcgtcgg
     3541 catcggcaac gcacccaccg cgctgatcga gctggtgcga ctgatccgcg atgaaggcgt
     3601 gcgcccggcg ctggtggtcg gcatgccggt gggcttcgtg tcggcggccg aatccaagga
     3661 ccggatggcc gcggtcgacg cggtgccgtg gatcgtcatc cgcggccgca agggcggctc
     3721 caccctggtg gtggcggcga tccacgccct gctcgggctg gccgagacac ggcagcgaca
     3781 ggccgtccgg ggctgaagcg acacgatggc ggcaggacat tccccccccg acaaggtgcg
     3841 caagggcgac gccaggcgcg cgcgcggcaa tcgtaccggc ctcaccaccg gcgccaactc
     3901 ggccgcggcc gcggcggccg ccacgctcgg cctggtgcgc ggcgcggtgc ccgacgcggt
     3961 ggagtgcgtg ctgcccaaca cgacgcgggt gcacttcacg atccgcgacg gccaggtgga
     4021 cggcgaccac gcccacgcgg tctcgatcaa ggacgccggc gacgaccccg atgccaccca
     4081 tggcgcccgt ctcaccgccg acgtgcgccg catccgcagg ggcggcggcg tggtgatcct
     4141 cgcgggcggc cgtggcgtgg gcgtggtcac caagcccggg ctcgggctgg cggtcggcgg
     4201 ccccgcgatc aaccccgtgc cgcggcgcaa catcatcgac aacgtgcgcg cggccggcgc
     4261 acccatcctc gaggccggcg acggtctgga ggtgacgatc tcggtgcccg gcggcgagga
     4321 gatggcgcag aagacgctca atgcccgcct cggcatcctc ggcggcatca gcatcctcgg
     4381 caccaccggc atcgtccgcc cgtattccac cgccgccttc cgcgccagcg tgatccaggc
     4441 catcgatgtc gccgccaacc agggccagac ctgcgtggtg ttcaccaccg gtggccgcac
     4501 cgagaaatgc gcgatgcgcg ccttcccgga cctcgacgag gcctgcttcg tgcagatggg
     4561 cgacttcgtc aaggccgcct tcaccaccgc ggtgaggcag ggcatgcgcc acatcgtcgt
     4621 cggcgccatg atcggcaagc tcaccaagat cgcccagggc ctg
//
LOCUS       DAOQNN010000257         4656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11360_length_4656_cov_11.123669, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000257 DAOQNN010000000
VERSION     DAOQNN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11360_length_4656_cov_11.123669"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKV97_12395"
     CDS             complement(<1..714)
                     /locus_tag="PKV97_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNB06725.1"
                     /translation="MRKLLTVTAVALALGTLFPQASALSAAHAQAAAPSMVSPNRFGL
                     PDFGDLVEQVGPAVVNIRVVQRAAQGTAGENPLANDPFYDFLRRFGVPMPGFPGQPGM
                     GPRARQGIGSGFIVSKDGYVLTNAHVVAGEDGDAALSEVTVTLIDKREFKAKVVGIDR
                     RTDVALLKLDASGLPAVKIGNPDQARVGEWVVAMGSPFGFDNTVTAGIISAKARRLPD
                     ETYVPFIQTDVAINPGNSGG"
     gene            complement(863..2899)
                     /locus_tag="PKV97_12400"
     CDS             complement(863..2899)
                     /locus_tag="PKV97_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB06726.1"
                     /translation="MHLHRPPTALALLSLLAAGTVEASSADAFFEPLPVVLSASRLAQ
                     PLQDSPGAVTVIDADLIAATGYRELARVLRLVPGFQVGQERGNLQWVTYHGLGMDSPM
                     QIQLLVDGRASLTPSFTASAERALPGDIERIEVVRGSNSAAYGSNAFLGIVNIITDDA
                     SRHPGTRLKLATGAPGIRDASLRHAVQSGPLSVRIGAHHEEDEGFADLYDGRRTDVLN
                     LRADLQVGEHDAVALNAGVAESTHELGYAGSIFDIAAQREGRDTNRSLHLRWRHGPED
                     DAIRVSAYWSRQSRDDLWRYDSAANSRTLANAFPERLRGTVGNSATFVQHNLEIERRE
                     RVDEATRLMWGAEWKRIEHDSPFWYHERRNHARDEHRLFANLELRTGTAFLWNAGGML
                     EQLQGDEARLAPRVFLNWQAAPRITWRIGHSRAWRQPDLFERNVDIRITDDKGELVQR
                     RQYPNPDLRPQRIDASEIGFLGVFGDSRSTLDVRLFRERIEDLIVRNAVPSDWPIEGK
                     LPEAISRGLGSTRWENHSGRVQLNGIETQLHLKPWRGTEFLLAHSLIDRDVDDPRIRR
                     NVAPYTASLSWLQRAGAWRSLLSVLRMGPIDAGFSYVPGFDYTVPSYTTVDWSVSRSL
                     RLFDMAVELRLTGINLLGRHQELANRPLQAQPEFAGRPANEVSRQAWLALETTF"
     gene            3029..3985
                     /locus_tag="PKV97_12405"
     CDS             3029..3985
                     /locus_tag="PKV97_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB06727.1"
                     /translation="MTLTDLRYLVALAHERHFGRAAEKCHVSQPTLSVAVKKVEEELG
                     IQLFERSASEVKITETGRRIVAQAERVLMEASQIQEIAAAGKDPLAGPLRVGVIYTIG
                     PYLLPRLIPRVHQLAPRMPLIIQENFTARLAEALKRGELDVIIISLPFDEAGVVAQPV
                     YDEPFRVLMPADHPWNAESEIDPQHLADDQLLLLGAGNCFRDQVLEVCPSCRNIGGLQ
                     RTLEGSSLETIRHMVATGLGVTVLPSSAADEMATQNPLVAVRPFTMPEPSRRVALAWR
                     VTYPRNGAIDVLRAAILDSALPGVRPVGRAVPPGQVPWVDSR"
     gene            4102..4572
                     /locus_tag="PKV97_12410"
     CDS             4102..4572
                     /locus_tag="PKV97_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HNB06728.1"
                     /translation="MEIDIGIKEKDRAKIAEGLSRLLADSYTLYLTTHNFHWNVTGPM
                     FNTLHLMFETQYNELALAVDEIAERIRALGFPAPGTYKEFQKLTSMAEPEGVPNAQEM
                     IRLLVQGQEAVVKTARSIAPIADKASDEPTLDLLTQRMQVHEKTAWMLRSLLEG"
BASE COUNT          718 a         1515 c         1679 g          744 t
ORIGIN      
        1 cccgccggag ttgcccgggt tgatcgccac gtcggtctgg atgaagggca cgtaggtctc
       61 gtcgggcagg cggcgcgcct tggccgagat gatgccggcg gtgaccgtgt tgtcgaagcc
      121 gaagggcgag cccatcgcca ccacccattc gcccacgcgg gcctggtcgg ggttgccgat
      181 cttcaccgcg ggcaggccgc tggcgtcgag cttgagcagg gcgacgtcgg tacgacggtc
      241 gatgccgacc accttggcct tgaactcgcg cttgtcgatc agggtgacgg tgacctcgga
      301 gagcgcggcg tcgccatcct cgccggcgac cacgtgggcg ttggtgagca cgtagccgtc
      361 cttgctgacg atgaagcccg agccgatgcc ctggcgcgcg cgcggaccca tgccgggctg
      421 gccggggaag cccggcatgg gcacgccgaa gcggcgcagg aagtcgtaga aggggtcgtt
      481 ggcgagcggg ttctcgcccg ccgtgccctg ggccgcgcgc tgcaccacgc ggatattgac
      541 caccgccggg cccacctgct cgaccaggtc gccgaagtcg ggcaggccga agcggttggg
      601 cgagaccatc gacggggcgg ccgcctgggc gtgcgcggcg ctcagcgccg aggcttgggg
      661 aaagagggtg ccgagcgcga gcgcgacggc ggtgacggtc aggagtttgc gcatgttgtc
      721 gggcatcctt gttcggcaac ggaaagattc ggcgccggag tcccggcggc cacctgtcgt
      781 ggacgcggcc tggcgtgcgc cgttcccgac gaaacctgcg ttttcaaatc gggtcgggca
      841 cgggcgcttc aagaggctgc gttcagaaag tggtttcgag cgcgagccag gcctggcggc
      901 tcacctcgtt ggcgggtcgg ccggcgaact cgggctgggc ctgcaggggg cgattggcga
      961 gttcctggtg gcggccgagc aggttgatgc cggtcaggcg gagctccacc gccatatcga
     1021 acagccgcag gctgcgcgag acgctccagt ccaccgtggt gtaggacggc acggtgtagt
     1081 cgaagccggg aacgtagctg aagccggcgt cgatcggccc catgcgcagc acgctgagca
     1141 gcgagcgcca cgcccctgca cgctgcagcc agctcaggct ggccgtgtag ggggcgacgt
     1201 tgcggcggat gcgtgggtcg tcgacgtcgc ggtcgatgag gctgtgcgcg aggaggaact
     1261 cggtgccgcg ccagggcttc aggtggagct gggtttcgat gccgttcagt tgcacgcggc
     1321 cgctgtggtt ctcccagcgc gtgctgccca gccctcgcga gatcgcctcg gggagcttgc
     1381 cctcgatcgg ccagtcggag ggaacggcgt tgcgcacgat caggtcttcg atgcgctcgc
     1441 ggaacaggcg gacgtcgagc gtgctgcggc tgtcgccgaa aacgccgaga aagccgatct
     1501 cgctggcgtc gatgcgctgg gggcgcaggt ccggattggg gtattgccgg cgctggacca
     1561 actcgccctt gtcgtcggtg atccgtatgt cgacgttgcg ctcgaaaagg tccggctgtc
     1621 gccatgcgcg cgagtggccg atgcgccagg tgatccgtgg agcggcctgc cagttcagga
     1681 acacgcgcgg cgcgagccgc gcctcgtcgc cttgcaactg ctccagcatg ccgcccgcgt
     1741 tccacaggaa ggcggtgccc gttcgcagct cgagattggc gaacaggcgg tgttcgtccc
     1801 gcgcatggtt gcggcgctcg tgataccaga agggcgagtc gtgctcgatg cgcttccatt
     1861 ccgcgcccca catcagccgc gtggcctcgt ccacgcgctc gcgccgctcg atctccaggt
     1921 tgtgctggac gaaggtggcg ctgttgccga ccgtgccgcg caggcgctcg gggaaggcgt
     1981 tcgccagggt ccggctgttt gccgcggagt cgtagcgcca gaggtcgtcg cgactctgcc
     2041 ggctccagta ggccgacacg cgtatcgcgt cgtcctcggg gccgtgccgc caccgcagat
     2101 gcagggatcg gttggtgtcg cgcccttcgc gctgggccgc gatgtcgaag atcgagccgg
     2161 cgtatccgag ttcgtgggtg ctctcggcga cccccgcgtt gagggcgacc gcatcgtgtt
     2221 cgcccacctg caggtcggcg cgaaggttca ggacgtcggt gcggcgaccg tcgtacaggt
     2281 cggcaaaccc ttcgtcttct tcatgatgcg cgccgatgcg cacgctgagg ggcccgctct
     2341 gcacggcgtg tcgcaggctc gcgtcgcgga tgccgggtgc gccggtggcg agcttcagcc
     2401 gggtgccggg atgtcggctg gcgtcgtcgg tgatgatgtt gacgatgccg aggaaggcgt
     2461 tggacccgta ggcggccgag ttcgagccgc gtaccacctc gatgcgttcg atgtctccgg
     2521 gcagggcacg ctcggcgctg gcggtgaagg atggcgtcag cgacgcgcgt ccgtcgacca
     2581 gcagctggat ctgcatcggc gagtccatgc cgaggccgtg gtaggtcacc cactgcaggt
     2641 tgccgcgctc ctggccgacc tggaatccgg gcaccaggcg cagcacgcgc gcgagctcgc
     2701 ggtagccggt cgcggcgatc aggtccgcat cgatcacggt gaccgcgccc gggctgtcct
     2761 gcaacggctg cgccagacgc gaggcgctca gcaccaccgg caggggctcg aagaaggcat
     2821 ctgcggacga cgcttcgacg gtgccggcag ccagcagcga cagcagggcg agagcggtgg
     2881 gggggcggtg gaggtgcatc ggaagcgggc ggctgcgcag aatgcgcgag tatacccagt
     2941 gcgctttcgg ggcgcgcagc aacgatcagt cggggtaaca tcgggcaccg tcgactccgg
     3001 gtgcacgccc ttccatcgtg tcgaagccat gaccctgacc gatctccgtt atctcgtcgc
     3061 gctcgcccat gagcgtcatt tcggccgcgc cgccgagaag tgccacgtgt cgcagcccac
     3121 gctgtcggtg gcggtgaaga aggtcgagga ggagctcggc atccagctct tcgagcgcag
     3181 cgcctcggag gtcaagatca ccgagaccgg gcgccggatc gtcgcccagg ccgagcgggt
     3241 gctgatggag gcgagccaga tccaggagat cgccgccgca ggcaaggacc cgctcgccgg
     3301 gccgctgcgt gtgggcgtga tctacaccat cggaccctac ctgctgccgc ggctgattcc
     3361 gcgcgtgcat cagctcgcgc cccggatgcc gctgatcatc caggagaact tcaccgcgcg
     3421 tctggccgag gcgctcaagc gcggcgaact cgacgtgatc atcatcagcc tgcccttcga
     3481 cgaggcgggc gtggtggcgc agcccgtgta tgacgagccc ttccgcgtgc tgatgccggc
     3541 cgaccatccg tggaatgcgg aaagcgagat cgacccccag catctcgccg acgaccagct
     3601 cctgctgctc ggtgccggca actgcttccg cgaccaggtg ctggaggtgt gcccgagctg
     3661 ccgcaacatc ggcgggctgc agcgcaccct cgagggcagc tcgctcgaaa ccatccgcca
     3721 catggtggcc accggcctcg gcgtgaccgt gctgccgagc tcggcagcgg acgagatggc
     3781 gacgcagaat ccgctggtcg cggtgcggcc tttcaccatg ccggagccct cgcgtcgcgt
     3841 cgcgctcgcc tggcgggtga cctatccgcg caacggggcg atcgacgtgc tgcgcgcggc
     3901 gatcctcgac agcgccctgc cgggcgtgcg tccggtgggc cgcgccgtgc cgcccgggca
     3961 ggtgccgtgg gtggattcgc gatgaaaatt cttgatcgga tccatcaggg taatcggttt
     4021 gatcaatgca tccctcaggc gctaacatga agcgcaaacc cctgcgggcg gtgtgaagcc
     4081 tgcatacaca ggagaaccag catggaaatc gacatcggca tcaaggaaaa ggaccgcgcg
     4141 aagatcgcgg agggcctgtc gcgtctgctc gccgacagct acacgcttta cctgaccacg
     4201 cacaacttcc actggaacgt gaccgggccg atgttcaaca ccctgcatct gatgttcgag
     4261 acgcagtaca acgaactcgc gctcgcggtg gacgagatcg ccgaacgcat ccgcgcgctc
     4321 ggcttcccgg cgccgggcac ctacaaggaa ttccagaagc tcaccagcat ggccgagccg
     4381 gaaggtgtgc cgaacgcgca ggagatgatc cgcctgctgg tgcagggtca ggaagcggtt
     4441 gtcaagaccg cccgcagcat cgccccgatc gccgacaagg cgagcgacga gcccacgctc
     4501 gacctgctca cccagcgcat gcaggtgcac gagaagaccg cctggatgct gcgcagcctg
     4561 ctcgaaggct gattcccgcc gcgcttgtgc agcaaatgaa cgggcccgcg gggcccgttc
     4621 ctgtatctgt ggcgactcgc gtcgctccta cggacg
//
LOCUS       DAOQNN010000258         4644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11414_length_4644_cov_10.567662, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000258 DAOQNN010000000
VERSION     DAOQNN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11414_length_4644_cov_10.567662"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="PKV97_12415"
     CDS             <1..1327
                     /locus_tag="PKV97_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNB06729.1"
                     /translation="VAIAFAIGAELAIAAHAGLIRERPWLTLLIAPAGFAALAWISRR
                     FFPGTEGSGIPQAIAASMSDDGRVRRQLLSLRIALAKVFLTLGGLLSGASVGREGPSV
                     QIGASVLHLLASRKRHRRIASSRDLIVAGSGAGIAAAFNTPLGGIMFAIEEMCRYRAF
                     QANSTTLTAVIFAGLMSLAVLGNYTYFGRTLASLGWPDGIWPVLACGVAGGLLGGIFT
                     RVLVASARGLPGRLGRIAEGQPVAFAAMCGLLTALIGLASGGVTYGTGYAESKAALEG
                     SAALPAAFTLMKLAVIWLVFASKIPGGIFAPALAVGAGLGADLALLLPGEQDAAILVL
                     GMVAFLAAMTQTPITSFVIVMEMTANHQMLLPLMATAVVAHGVARSVAPVPLYHALSH
                     PWLRRAEKRAHEKRPRPAHPPAGAQGAGAARPAVEPPSSADAGAPDQRL"
     gene            complement(1292..2245)
                     /locus_tag="PKV97_12420"
     CDS             complement(1292..2245)
                     /locus_tag="PKV97_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB06730.1"
                     /translation="MNNPNSMFARAMPILFGALFLIGLVAPFAVYPTFLMKVLCFGLF
                     ACAFNLLLGFAGLLSFGHAAFLGSAGYVCGMLVRDLGVTPELGILGGTLAAGALGWVF
                     GVLAIRRTGIYFAMITLALSQMVYFVVLQWKATGGEDGLQGVPRGHLFGVIDLSNNLA
                     MYYLVFAVFCIGFFIIYRAIHSPFGQILKAIRENEPRAMSLGYDVAKFKLLAFVLSAA
                     LAGMAGATKTLVFQLASLTDVHWHMSGEVVLMTLLGGLGTVLGPLVGAATIVSLQTEL
                     ADSVGSWVTVIMGTIFVVCVLLFRRGIVGELQALIRRAGIR"
     gene            complement(2269..3150)
                     /locus_tag="PKV97_12425"
     CDS             complement(2269..3150)
                     /locus_tag="PKV97_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB06731.1"
                     /translation="MEIFGVPSALLGSQLLVGLINGSFYAILSLGLAIIFGLLNIINF
                     THGAQYMMGAFVAWIALNKLGINYWVALLFAPIVIGALGVLIERTMLRKLYKLDHLYG
                     LLLTFGLALIFEGIFRDQYGISGQSYPVPELLQGGVNLGFMFLPIYRGWVVVAALTVC
                     FATWFVIEKTKLGAYLRAGTENAQMVQALGINVPLLITFTYGFGVALAAFAGVLAAPV
                     YQVSPLMGSNLIIVVFAVVVIGGMGSIMGSILTGLGLGLIEGLTRVFYPEASAVVVFV
                     IMVIVLLVRPAGLFGKA"
     gene            complement(3222..4436)
                     /locus_tag="PKV97_12430"
     CDS             complement(3222..4436)
                     /locus_tag="PKV97_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB06732.1"
                     /translation="MQMKKLTTLACAAAFAMLSGAASAQISGNTVKIGVLTDMSGTYS
                     DLAGAGAVLATQMAIEDFIAEAKPAFKVEMVSADHQNKADIASNKAREWFEREGVDTA
                     TELVTTSTALAVMKIAKEMNRVALMSGPASTPITNEQCNDVTVHYTYDTYALANGTAK
                     AVTQQGGNTWFFLTADYAFGQALEKDSSAVVTANGGKVLGSVRHPFPASDFSSFLLQA
                     QASGAKIIGLANAGADTTNAIKQAAEFGVTPKQSLAGLLMFISDVHSLGLQATQGMYL
                     TTGFYWDLDDQTRAWSKRFFEKQKRMPTMVQAGQYSSVLHYLRAVQATGTDEAGKVMA
                     QMKATPIKDFFGKNGRIREDGRMVHDMYLAQVKTPAESKYPWDYYHIRQTIPAEEAFQ
                     PLAQSKCPLVKK"
BASE COUNT          751 a         1577 c         1598 g          718 t
ORIGIN      
        1 ggtcgcgatc gccttcgcga tcggcgccga gctggcgatc gccgcccacg ccgggctgat
       61 ccgcgagcgc ccctggctca ccctgctgat cgcgcccgcc ggcttcgccg cgctggcgtg
      121 gatctcgcgc cgcttcttcc ccggtaccga gggcagcggc atcccgcagg cgatcgcggc
      181 gtcgatgtcg gatgacggcc gcgtgcgccg ccagctgctg tcgctgcgca tcgcgctggc
      241 caaggtcttc ctgaccctgg gcgggctgct cagcggcgcc tcggtgggtc gcgagggccc
      301 ttcggtgcag atcggcgcct cggtgctgca cctgctcgcc agccgcaagc gccaccgccg
      361 catcgcctcc agccgcgacc tcatcgtcgc cggcagcggc gccggcatcg ccgcggcctt
      421 caacaccccg ctcggcggca tcatgttcgc gatcgaggag atgtgccgct accgcgcctt
      481 ccaggccaac agcaccacgc tcaccgcggt gatcttcgcc ggcctgatgt cgctcgccgt
      541 gctgggcaac tacacctact tcggccgcac cctggcgagc ctgggctggc ccgacgggat
      601 ctggccggtg ctcgcctgcg gggtggccgg aggcctgctc ggcggcatct tcacgcgcgt
      661 gctggtggcg tccgcacgcg gcctgcccgg ccgcctcggt cgcatcgccg agggccagcc
      721 ggtcgccttc gcggcgatgt gcggcctgct caccgcgttg atcggcctgg ccagcggcgg
      781 cgtcacctac ggcacgggct atgcggaatc caaggccgcg ctcgagggca gcgcggcctt
      841 gccggcggcc ttcacgctga tgaagctggc ggtgatctgg ctggtcttcg cgtccaagat
      901 ccccggcggc atcttcgccc ccgcgctcgc ggtgggcgcc ggcctgggcg ccgacctcgc
      961 cctgctgctg ccgggcgagc aggacgcggc gatcctggtg ctgggcatgg tggccttcct
     1021 cgcggcgatg acgcagacgc cgatcacctc cttcgtgatc gtcatggaga tgaccgccaa
     1081 ccaccagatg ctgctgccgc tgatggccac cgcggtggtg gcccacggcg tggcgcgctc
     1141 ggtggcgccg gtaccgctct accacgcgct ctcccacccc tggctgcggc gcgccgagaa
     1201 gcgggcgcac gaaaaacgcc cgcgccctgc gcaccccccg gcgggtgcgc agggcgcggg
     1261 cgccgcgagg ccggctgtgg aaccgccgtc ctcagcggat gccggcgcgc cggatcagcg
     1321 cctgtagctc gccgacgatg ccgcggcgga acagcagcac gcagaccacg aagatcgtgc
     1381 ccatgatcac cgtgacccag gagcccacgc tgtcggcgag ctcggtctgc agcgacacga
     1441 tggtggcggc gccgaccagc gggccgagca cggtgccgag gccgccgagc agcgtcatca
     1501 gcaccacctc gcccgacatg tgccaatgca cgtcggtgag cgaggcgagc tggaacacca
     1561 gggtcttggt cgcccccgcc atgccggcga gcgcggccga gagcacgaag gcgagcagct
     1621 tgaacttggc cacgtcgtag ccgagcgaca tcgcgcgcgg ctcgttctcg cggatcgcct
     1681 tgaggatctg cccgaagggc gaatggatcg cacggtagat gatgaagaag ccgatgcaga
     1741 acaccgcgaa cacgaggtag tacatcgcga ggttgttcga caggtcgatg acaccgaaca
     1801 gatgtccgcg cggtacgccc tgcaagccgt cctcgccacc ggtcgccttc cactgcagca
     1861 cgacgaaata caccatctgc gacagcgcca gcgtgatcat cgcgaagtag atcccggtac
     1921 ggcggatcgc cagcacgccg aagacccagc cgagcgcgcc ggcggcgagc gtgccgccga
     1981 ggatgccgag ctcgggcgtc acccccaggt cgcgcaccag catgccgcac acgtagccgg
     2041 cgctgccgag gaaggcggca tggccgaagg agagcagccc ggcgaagccg agcagcaggt
     2101 tgaacgcgca cgcgaacagc ccgaagcaca ggaccttcat cagaaaggtg ggatacaccg
     2161 cgaacggcgc caccagcccg atcaggaaca gcgcgccgaa caggatcggc atggcgcggg
     2221 cgaacatcga attggggttg ttcatcagtc tgattccttt gcccgcgctc aggccttgcc
     2281 gaacaggccg gcgggccgca ccagcagcac gattaccatg atcacgaaca ccaccactgc
     2341 cgaagcctcc gggtagaaca cccgcgtcag cccctcgatc agccccaggc cgaggccggt
     2401 gaggatggag cccatgatcg aacccatgcc gccgatcacg accaccgcga acaccacgat
     2461 gatgaggttg gagcccatca gcggactgac ctggtacacc ggcgccgcga gcaccccggc
     2521 gaaggcggcg agcgcgacgc cgaagccgta ggtgaaggtg atcagcagcg gcacgttgat
     2581 gccgagcgcc tgcaccatct gcgcgttctc ggtgccggcg cgcaggtagg cgccgagctt
     2641 ggtcttctcg atgacgaacc aggtggcgaa gcacaccgtc agcgcggcca ccacgaccca
     2701 gccgcggtag atcggcagga acatgaagcc gaggttcacc ccgccctgca gcagctcggg
     2761 caccgggtag gactggccgg agatgccgta ctggtcgcgg aagatgccct cgaagatcag
     2821 cgccaggccg aaggtgagca agaggccgta gaggtggtcg agcttgtaga gcttgcgcag
     2881 catcgtgcgc tcgatcagca cgccgagcgc gccgatgacg atgggcgcga acagcagcgc
     2941 gacccagtag ttgatgccga gcttgttgag cgcgatccac gccacgaagg cgcccatcat
     3001 gtactgggcg ccgtgggtga agttgatgat gttgagcagg ccgaagatga tggccaggcc
     3061 caggctcagg atcgcgtaga acgaaccgtt gatgaggccg accagcagct ggctgccgag
     3121 cagcgcgctc ggaacgccga agatttccat gtgcgactcc cgcagcggga cggccgtgcc
     3181 gccccgctgc gtcgtgaagt gcgggacgcc ccgcgatcag ctcacttctt gaccagcggg
     3241 cacttcgact gcgccagcgg ctggaaggcc tcttccgccg ggatggtctg gcggatgtgg
     3301 tagtagtccc acgggtactt cgactcggcg ggcgtcttca cctgcgccag gtacatgtcg
     3361 tgcaccatgc gcccatcctc gcggatgcgg ccgttcttgc cgaagaagtc cttgatcggg
     3421 gtggccttca tctgcgccat gaccttgccc gcctcgtccg tgccggtggc ctgcaccgcg
     3481 cgcaggtagt gcagcaccga ggagtactgg ccggcctgca ccatggtcgg catgcgcttc
     3541 tgcttctcga agaagcgctt ggaccaggca cgcgtctggt cgtcgagatc ccagtagaag
     3601 ccggtggtca ggtacatgcc ctgggtggcc tgcaggccga gcgagtgcac gtcggagatg
     3661 aacatcagca ggccggcgag cgactgcttg ggcgtgacgc cgaactcggc cgcctgcttg
     3721 atcgcgttgg tggtgtcggc gccggcgttg gccagaccga tgatcttggc gcccgaggcc
     3781 tgggcctgaa gcaggaagga cgagaagtcg gaagccggga agggatgacg taccgagccg
     3841 agcaccttgc cgccgttggc cgtcaccacc gcggacgaat ccttctccag cgcctgaccg
     3901 aaggcatagt cggcggtcag gaagaaccag gtgttgccgc cctgctgcgt caccgccttg
     3961 gcggtgccgt tggcgagcgc ataggtgtcg taggtgtagt gcaccgtcac gtcgttgcac
     4021 tgctcgttgg tgatcggcgt cgaggccggg ccgctcatca gcgcgacgcg gttcatctcc
     4081 ttggcgatct tcatcaccgc gagcgcggtc gaggtggtga cgagctcggt agccgtgtcg
     4141 acgccctcgc gctcgaacca ttcgcgcgcc ttgttggaag cgatgtcggc cttgttctgg
     4201 tggtcggcgg agaccatctc gaccttgaag gccggcttgg cctcggcgat gaagtcctcg
     4261 atcgccatct gggtggcgag caccgcgcct gcgccggcaa ggtcggaata ggtgcccgac
     4321 atgtcggtga gcacgccgat cttgaccgtg ttgcccgaaa tctgcgcact cgccgcgccc
     4381 gacagcatgg cgaaggcggc cgcgcaggcc agggtggtga gtttcttcat ctgcatcgtg
     4441 ggtctccgtc tcgttcgtgt ggttgttacg cagggactgc gcggtgcagc cgctcagacc
     4501 cccagcgtct ggtgcagcca gtccatcttc ccggggagct cctccgcggt gatggtggcg
     4561 atgatctcgc cgtgctcgag cacgtagtgg cggtcggcca gcggggcggc gaagcggaag
     4621 ttctgctcca ccagcacgat ggtg
//
LOCUS       DAOQNN010000259         4635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11448_length_4635_cov_9.776856, whole genome shotgun sequence.
ACCESSION   DAOQNN010000259 DAOQNN010000000
VERSION     DAOQNN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11448_length_4635_cov_9.776856"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..510)
                     /locus_tag="PKV97_12435"
     CDS             complement(121..510)
                     /locus_tag="PKV97_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06733.1"
                     /translation="MLLLPTHDVAAEELVLIVNRDSGISALSREQASHLFLGRVKMLP
                     SGARANVVEVEPLRASFYRRLLGRDIAEINAYWARLQFSGRTQPPLRVADSAAAISRV
                     AQDPDAIAFVDLVPDDPRARVVLRLGP"
     gene            complement(554..1819)
                     /locus_tag="PKV97_12440"
     CDS             complement(554..1819)
                     /locus_tag="PKV97_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal protein"
                     /protein_id="HNB06734.1"
                     /translation="MRPVCIAAIAAMSLCATSAAALAAGRPVQADAPRWSASGFGTLG
                     AAWHDEDDTQFRRSVDQHRGTRANELDFGVDSSLGLQVHGALTPQWSVMAQAVMNQGR
                     HGEWGPHLVWGFVKHVPSDWLELRAGRLVTDIYLDGDSRHVGYAYTTVRPYADVYGRL
                     TYDNFDGLDATLQHALGDGLLRFKLFGGRTRGSVFLNRMEHPVPIGRSFGVTLDWIGP
                     ELSLKLSWGNMVSTRNAIYRELPFTLRHLASLAPGTEFAGQAVARASEIAGSNRIGYL
                     GAALGWERGPFSLQLMATDMSMSVYPRFEGWGTGATAAYRVGRWKPYLTGSRSILDPV
                     DRALDVPPALDPAGQLRGFWQTVHGYTRHDQTTIGVGVRYDFADNMALKLQFDRIDAK
                     RSSALLDDRGFVTGPRSPTVFSATLDFVF"
     gene            2017..3096
                     /locus_tag="PKV97_12445"
     CDS             2017..3096
                     /locus_tag="PKV97_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HNB06735.1"
                     /translation="MERKDFTRPVHVTTWMAVFLALVAALAAALVPQLKHAFIANAAF
                     NGVILFVLAVGIAVNLRQVWRLQREVLWIEEFQHSAGGGSATGLQPVLLAPMARVLSN
                     RRRGQAHLSPASTRSILDGVQIRLEEQRDLSRYLIGLLIFLGLLGTFWGLLATIRSIG
                     EIIGSLSVGSDPVAMFEALKAKLDAPLGGMATSFSTSLFGLAGSLVVGFLDLQSGHAQ
                     NRFYNELEDWLSHMTKLPSGVGAEGEGNVPAYVQALLEQTAESIERMQRSAAESERER
                     RATAEHLGELNTQLTRLADLIGRESRDLSTLSTTQDELRGLLRQLAQQPAQGVQFTED
                     LRAELRLLSRTIGAALGGAGAGKAD"
     gene            3100..4224
                     /locus_tag="PKV97_12450"
     CDS             3100..4224
                     /locus_tag="PKV97_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HNB06736.1"
                     /translation="MARLARRRPLDFWPGFVDALASLLMVMVFVILIFVIGQFVLADA
                     VSGRDRALAQLEAELATLARTLSLEQSARQRAEAEVGELSASLSAARRESEARGNELL
                     AARDELHAAQDEARSRREEATRIVADIEALQRLKTELEAEAARLASALDTSERGLKEH
                     KEMSAAAIAQVELLNRQLAAVREQLEQLNAALDAAKLAAKDKDLKLEELGRELNLALA
                     GRVKELARYRSEFFGRLQEVLGKRKDVQIVGDRFVFSSEVLFASASDEVSADGMVQLT
                     RLAETLKTLSADMPKDLPWVLQVDGHTDRRPIATARFPSNWELSTARALAIVKFLRGQ
                     GIPPERLAATGYGEFHPLDARSTEEAYTRNRRIELKLTSR"
     gene            complement(4247..>4635)
                     /locus_tag="PKV97_12455"
     CDS             complement(4247..>4635)
                     /locus_tag="PKV97_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNB06737.1"
                     /translation="LFPAISLLLLAYTNRFLTLAQVIRQLNASPDRGAALVQCQLPGL
                     KRRITLTQYMQGFGVLSFLLCALSMLALFLEAEVAGKWMFGTSIVTLAVSLVLSLVEV
                     LLSTEALSLVVRDLEQAKREGGTPCS"
BASE COUNT          779 a         1687 c         1521 g          648 t
ORIGIN      
        1 gcgaagccgt actgcgactt gccggcctgt cggccgatcc cgcgcgtgac gccacgcccg
       61 acccgcttgg cgctaccgat cgcggcgatg ccgcccccca ccggccggcc gacggcgggc
      121 tcagggacca aggcgcagca ccacccgcgc gcggggatcg tcggggacga gatcgacgaa
      181 cgcgatcgca tccggatcct gggcgacccg cgaaatcgcg gccgcgctgt cggcgacccg
      241 cagcggcggt tgtgtccgcc cggagaactg caatcgcgcc cagtacgcgt ttatctcggc
      301 gatgtcgcgc ccgagcaggc ggcgatagaa gcttgcgcgc aacggttcca cttcgaccac
      361 gttggcacgc gcccccgaag gcagcatctt cacgcgcccg aggaagagat ggctggcctg
      421 ctcgcgactc agggcggaga tgccgctgtc gcgattgacg atgaggacga gttcctcggc
      481 cgccacgtcg tgcgtcggga gcagcagcac gaggagcgca gcgacgagcg tatggattcg
      541 catggccgga cgctcagaag acgaaatcca gcgttgcgga aaacaccgtc ggcgagcgcg
      601 ggccggtgac gaaaccacga tcgtcgagca gcgcggagga acgcttcgcg tcgatgcgat
      661 cgaactgcag cttcaaggcc atgttgtcgg cgaagtcata gcgcacgccc acgccgatgg
      721 tggtctggtc gtggcgggtg tagccgtgga cggtctgcca gaaaccgcgc agctggcccg
      781 cggggtccaa cgccggcggc acgtcgaggg cccgatcgac aggatcgagg atgctgcgcg
      841 agccggtaag gtaaggcttc cagcgcccga cccgataggc ggcggtcgcc cccgtccccc
      901 aaccttcgaa gcgcgggtac acggacatcg acatgtcggt ggccatgagc tgcagcgaga
      961 acggtccgcg ctcccagccg agcgccgccc ccaggtaacc gatccggttg gaccccgcga
     1021 tctcggatgc gcgggccacg gcctgccctg cgaattccgt ccccggggcc aggctcgcga
     1081 ggtgacgcag cgtgaacggc agctcccgat agatggcgtt gcgcgtggac accatgttgc
     1141 cccaggacag tttcaggctc agttccggcc cgatccagtc cagggtgacg ccgaagctgc
     1201 ggccgatcgg caccggatgc tccatccggt tcaggaacac gctgccgcgc gtacgcccgc
     1261 cgaacagctt gaagcgcagc aggccgtcgc cgagcgcgtg ctggagggtc gcatccaggc
     1321 cgtcgaagtt gtcataggtc agacggccgt acacgtcggc atacggcctc accgtcgtgt
     1381 aggcgtagcc gacgtggcgc gagtcgccgt cgaggtagat atcggtgacc aggcgaccgg
     1441 cgcgcagttc gagccaatcg ctgggcacgt gcttgacgaa gccccatacc aggtgcggtc
     1501 cccactcgcc gtggcgcccc tggttcatga ccgcctgcgc catgaccgac cactgcggcg
     1561 tcaacgcacc gtgcacctgc aggccgagcg agctgtcgac accgaagtcg agttcgttcg
     1621 cacgcgtgcc acgatgctgg tccacgctgc ggcggaactg cgtatcgtcc tcgtcgtgcc
     1681 aggcggcgcc gagcgtgccg aagccgctcg cgctccagcg cggggcgtcg gcctgcacag
     1741 gccgtcctgc cgccagcgcg gcggcgctcg tcgcgcacaa gctcatcgcc gcgatcgcgg
     1801 cgatgcaaac gggtctcacg gtcgacaccg ggcaacacct ctcccgatcg cggatcgggt
     1861 ccgagtgagc aaaaggctcg aagatagcga gcccaccgcc cctcatccca tgacatagat
     1921 cacgactccg ccatttaacg cggttcgcgc tagactcgca gtcgccacgc gaccccgaat
     1981 gggcactcgt cacccaaagc tccggaaaaa cacccgatgg aacgcaagga tttcaccaga
     2041 cccgtccatg tcacgacctg gatggccgtg tttctcgccc tcgtcgccgc gctcgccgcc
     2101 gccctggtcc cgcaactcaa gcacgccttc atcgccaacg ccgcattcaa cggcgtgatc
     2161 ctcttcgtcc tcgcggtcgg catcgcagtc aatctgcgcc aggtgtggcg gctgcagcgt
     2221 gaggtgctgt ggatcgagga attccagcac agcgccggcg gcggctcggc caccggcctg
     2281 cagccggtgc tgctcgcccc gatggcacgt gtgctctcca accgccgtcg cggccaggcc
     2341 cacctgtcgc cggcctccac ccgctccatc ctcgacggcg tgcagatccg cctcgaggag
     2401 cagcgcgacc tctcgcgcta cctgatcggc ctgctgatct tcctcggcct gctcggcacc
     2461 ttctggggcc tgctcgccac catccggtcc atcggcgaga tcatcggcag cctgagcgtc
     2521 ggcagcgatc ccgtcgccat gttcgaggcg ctcaaggcca agctcgacgc gccgctcggc
     2581 ggcatggcga cgagcttctc gacctcgctc ttcggcctcg ccggctcgct cgtggtcggc
     2641 ttcctcgacc tgcaatccgg ccatgcacag aaccgcttct acaacgagct cgaggactgg
     2701 ctctcgcaca tgaccaagct gccctccggc gtcggcgccg agggcgaggg caacgtgccc
     2761 gcctacgtcc aggccctgct cgagcagacc gccgaaagca tcgagcgcat gcagcgctcg
     2821 gccgcggaat ccgagcgcga gcgccgcgcc accgccgagc acctcggcga gctcaacacc
     2881 cagctcaccc gcctggccga tctcatcggg cgcgaatcgc gcgacctgtc cacgctgtcc
     2941 accacccagg acgagctgcg cgggctgctg cgccagctcg cccagcagcc cgcccagggc
     3001 gtgcaattca ccgaagacct gcgcgccgag ttgcgcctgc tgtcgcgcac gatcggcgcc
     3061 gcgctcggtg gtgccggcgc cggcaaggcg gactgagcca tggcccgact cgcgcgccgc
     3121 cggccgctgg atttctggcc cggcttcgtc gacgccctcg cctcgctgct gatggtgatg
     3181 gtgttcgtga tcctgatctt cgtcatcggc cagttcgtgc tcgccgacgc ggtgagcggc
     3241 cgcgaccgcg cgctcgccca gctcgaggcc gagctcgcca ccctcgccag gacactgagc
     3301 ctggaacaga gcgcgcgcca acgagcggag gccgaggtcg gcgagctctc ggcctcgctg
     3361 agcgccgccc ggcgcgagag cgaagcgcgc ggcaacgagc tgctcgccgc gcgcgacgag
     3421 ctccacgccg cccaggacga ggcgcgcagc cgccgcgagg aggccacccg catcgttgcc
     3481 gacatcgagg ccctgcagcg cctgaagacc gagctcgagg ccgaggccgc ccgcctggcg
     3541 agcgcactcg acacctcgga gcgcggcctg aaggaacaca aggagatgtc cgccgccgcg
     3601 atcgcccagg tcgagttgct caatcgccag ctcgccgcgg tgcgcgagca actcgagcag
     3661 ctcaatgcgg cactcgacgc cgccaagctc gcggccaagg acaaggatct gaagctcgag
     3721 gagctcggcc gcgagctcaa cctggcgctt gccggccgcg tcaaggaact cgcacgttac
     3781 cgctccgagt tcttcggccg cctgcaggag gtgctcggca agcgcaagga cgtgcagatc
     3841 gtcggcgacc gcttcgtctt ctccagcgag gtgctgttcg cctccgcctc ggacgaggtc
     3901 agcgccgacg gcatggtcca gctcacccgc ctggccgaga cgctgaagac gctgtccgcc
     3961 gacatgccca aggacctgcc ctgggtcctg caggtggacg gccacaccga ccgccgcccg
     4021 atcgccaccg cccgcttccc gtcgaactgg gagctatcga ccgcccgcgc cctcgccatc
     4081 gtcaagttcc tgcgcggcca gggcatcccg cccgagcgcc tggccgcgac cggctacggc
     4141 gagttccacc cgctcgatgc gcgcagcacc gaggaagcct acacgcgcaa ccgccgcatc
     4201 gagctcaagc tgacaagccg gtgaggcgac gcaaaggggc acgacgtcag gagcaaggcg
     4261 tccccccttc ccgcttcgcc tgctccaggt cgcggacgac cagcgatagc gcctcggtcg
     4321 acaacaacac ctcgaccagc gaaagcacca acgacaccgc gagcgtcacg atgctggtgc
     4381 cgaacatcca tttgccggcc acctcggcct cgaggaagag cgcgagcatg gacagcgcgc
     4441 agagcaggaa gctcagcacg ccaaagcctt gcatgtactg ggtgagggtg atccggcgct
     4501 tgagcccggg gagctggcac tgcaccagcg cggcgccgcg atcgggcgag gcgttgagct
     4561 ggcggatcac ctgggcaagc gtgaggaagc ggttggtgta ggcgagcagc agcagcgaga
     4621 tcgccgggaa gagca
//
LOCUS       DAOQNN010000260         4621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11500_length_4621_cov_9.472843, whole genome shotgun sequence.
ACCESSION   DAOQNN010000260 DAOQNN010000000
VERSION     DAOQNN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11500_length_4621_cov_9.472843"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKV97_12460"
     CDS             <1..452
                     /locus_tag="PKV97_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB06738.1"
                     /translation="DPGNLGSLLRTAAAAGIRHALLGEGCAQAWSPRALRAGMGAQFV
                     VRIEEGCELLARLESYPGLCLATALREDAHPLYELDLRGPVAWLFGAEGQGLSPALLA
                     RADGCVIIPMAAGIESLNVAAAAAVCLFEQARQLRATGTIRGVNFPS"
     gene            564..1076
                     /locus_tag="PKV97_12465"
     CDS             564..1076
                     /locus_tag="PKV97_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06739.1"
                     /translation="MEKYAKTPKGQDEIAHRTHGLGLRARRLLILVDGQRSVEDLCAL
                     TGDAGIAAALGELAEAGFIAPSAGMADPPEASIREGQPAAGAAPVARAHTGHTASLPL
                     ARDFMINTLRTFHGPYGKLGLVKRVHATRSSSELRALIAEWKESIDESRLGRKRADEL
                     LALLEEVLPG"
     gene            1149..1943
                     /gene="yaaA"
                     /locus_tag="PKV97_12470"
     CDS             1149..1943
                     /gene="yaaA"
                     /locus_tag="PKV97_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNB06740.1"
                     /translation="MILVISPAKALDYETPITVSTYTRPDFLDDSAALIEVLREKSPA
                     EIAELMSLSDPLSTLNVARYASWSRPFSPDNARQALLAFNGDVYEGLDARSLTADELG
                     WAQDHLRILSGLYGVLRPLDLMQAYRLEMGTRLANPRGKNLYEFWGERITDALNGLLA
                     REEDAGRERVLLNLASDEYFKSVKPKKLKGRIVTPVFEDWKGGRYKIVSFYAKRARGL
                     MSRYVITNRIDEVDALQGFDAEGYAFAADASDADTLVFRRREEGTC"
     gene            1940..3076
                     /locus_tag="PKV97_12475"
     CDS             1940..3076
                     /locus_tag="PKV97_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HNB06741.1"
                     /translation="MKISSSFDSGAIEVVRLDDPADIRLRLRKDNAADFHQWFHFRLM
                     GAAGKPVRMVFENAAGAAYPDGWRDYRCVASYDRQNWFRIAGTRYEDGQLIVEHTPER
                     DSLYYAYFEPYSHERHLDLLARVELSPYARVRNLGSTVDGRDLDVVTVGRDAPGRVPV
                     WIIARQHPGETMAEWFVEGLLERLLDNADPVARKVREHAVLHIVPNMNPDGAVRGNLR
                     TNAAGRNLNREWRAPDPVASPEVFLVRAEMERTGCALFLDIHGDESLPYVFFSTAEEV
                     PGFTAAAAAAQARFVETLLMVSPDFQTQEGYKPGRFGEELLTLASKWVANQFGCVSLT
                     LEMPFKDNIILPDGHVGWNGARSKRLGAAMLDPILRHLREGGEG"
     gene            complement(3173..4498)
                     /gene="hpnE"
                     /locus_tag="PKV97_12480"
     CDS             complement(3173..4498)
                     /gene="hpnE"
                     /locus_tag="PKV97_12480"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HNB06742.1"
                     /translation="MSVPVVAIIGAGYAGLACGVELARAGVHVTVFERSHTMGGRARV
                     VHKDHHWRVDNGQHILIGAYSELTRLLRLTGVSPKQLAHLPLTLHVPGRLHLKAASLP
                     APFHLAVGLLRAQGLHWADRLAMLRLMRWLKRRGFRLPDPGMTVDALLAETRQTPTLC
                     QLIWEPLCVAALNTPAAAASAQVFANVLRDSLGAGAAASELLLPRTDLSELFPVPAAR
                     YIGVRRGRLRTGNAIEAVERVAGGYRLDGDPGSHPWPHVVVATAPYHAGALLASVGGC
                     ERLVAQIDALEHEPIVSVYLALGRGQQLPEPMIGLASGSTAGPAQWVFDRGQLGGPEG
                     LFSCVISAHGPHEALARDELVLAVHAQLERQLGRRLPAPEWSQVIVEKRATFACRPGV
                     FRPGIRTPLPGLWLAGDYLDSDYPATLESAVRSGVACATAILAEIGHKA"
BASE COUNT          712 a         1633 c         1641 g          635 t
ORIGIN      
        1 aggaccccgg caacctcggc agcctgctgc gcaccgcggc cgcggccggc atccgccacg
       61 cgctgctcgg tgagggctgc gcgcaggcct ggtcgccgcg cgcgctgcgt gccggcatgg
      121 gcgcgcagtt cgttgtccgg atcgaggagg gctgcgaact gctcgcccga ctggagagct
      181 acccgggtct gtgcctggcc actgccctgc gcgaggacgc ccacccgctc tacgagctcg
      241 acctgcgcgg cccggtggcc tggcttttcg gggcggaagg gcagggactt tccccggccc
      301 tgctggcgcg tgccgacggc tgcgtcatca tcccgatggc ggccggcatc gaatccctca
      361 acgtcgccgc ggccgcggcg gtctgtctgt tcgagcaggc ccgtcagctc cgcgccacgg
      421 ggacgatccg aggcgtgaat tttccttctt gactcgggcg caggcttgct tccatgcttt
      481 ccgcattcca agcggggcac ggcggacgcc gcgacagacc ccgaggacga gaccaggggt
      541 ggtagcgccc cgcgaggagg atgatggaaa agtacgccaa gacacccaag ggacaggacg
      601 agatcgcgca tcgcacgcac ggcctcgggc tgcgcgcccg ccgcctgctg atcctggtcg
      661 atggacaacg cagcgtcgag gatctgtgtg cccttaccgg cgacgcgggg attgcggctg
      721 cgctcggaga actcgccgag gcagggttca tcgcgcccag cgccgggatg gccgacccgc
      781 ccgaagccag catacgcgaa ggacagccgg cggcaggcgc ggcgccggtc gcgcgggcgc
      841 acaccggcca cacggccagc ctgccgctcg cgcgcgactt catgatcaac accctgcgca
      901 ccttccacgg cccctacggc aagctcggcc tggtcaagcg tgtccatgcg acccggagca
      961 gcagcgaact gcgcgcgctg atcgccgaat ggaaggagtc gatcgacgag tcacgcctcg
     1021 gccgcaagcg ggcggacgag ctgctcgcgc tgctggagga ggtgctgccg ggctgaaggc
     1081 ggtgttgcgg ggcgattcgc gttaacatcc ggccatccgg ctccgcgccg acacagcgaa
     1141 cagaaggcat gatcctcgtc atctcccccg ccaaggcgct cgactacgag acgccgatca
     1201 ccgtctccac ctacacccgg cccgacttcc tcgacgattc cgcagcgctg atcgaggtac
     1261 tgcgcgagaa atcgcccgcc gagatcgccg agctgatgtc gctctcggat ccactctcca
     1321 cgctcaacgt ggcgcgctac gcgagctggt cgcgcccatt cagccccgac aacgcacggc
     1381 aggcgctgct ggccttcaac ggcgacgtct acgagggact cgatgcgagg agcctgaccg
     1441 cggacgaact tggctgggcg caggaccacc tgcgcatcct ctccggcctc tacggcgtgc
     1501 tgcgcccgct cgacctgatg caggcctacc gcctggagat gggcacccgg ctggccaacc
     1561 cgcgcggcaa gaacctctac gagttctggg gcgagcgcat caccgacgcg ctcaacggcc
     1621 tgctcgcgcg cgaggaagac gccgggcgcg agcgtgtgct gctgaacctc gcctccgacg
     1681 agtacttcaa gtcggtcaag cccaagaagc tgaaggggcg catcgtcacc ccggtgttcg
     1741 aggactggaa gggcgggcgc tacaagatcg tcagcttcta cgccaagcgc gcgcgcgggc
     1801 tgatgagccg ttacgtcatc acgaatcgca tcgacgaggt cgacgcactg cagggcttcg
     1861 acgccgaggg ctacgccttc gccgccgacg cgtccgacgc cgataccctg gtcttccgtc
     1921 gtcgcgagga gggaacgtgc tgaagatcag ctccagcttc gactccggcg cgatcgaggt
     1981 cgtgcgcctc gacgatccgg ccgacatccg cctgcgcctg cgcaaggaca acgctgccga
     2041 cttccaccag tggttccact tccgcctgat gggcgcggcc ggcaagccgg tgcgcatggt
     2101 gttcgagaat gccgcgggcg cggcctatcc ggacggatgg cgggactacc gctgcgtcgc
     2161 ctcctacgac cgccagaact ggttccgcat cgccggcacg cgctacgagg acgggcagct
     2221 gatcgtcgag catacgcccg agcgcgacag cctctactac gcctacttcg agccctattc
     2281 gcacgagcgt cacctcgacc tgctcgcgcg cgtcgagctc tcgccctacg cccgcgtgcg
     2341 caacctcggc agtacggtgg atgggcggga cctcgacgtg gtgacggtcg ggcgcgacgc
     2401 gccgggacgg gtgccggtgt ggatcatcgc gcgccagcat cccggcgaga ccatggcgga
     2461 atggttcgtc gagggcctcc tcgagcgcct gctcgacaat gccgacccgg tcgcgcgcaa
     2521 ggtgcgcgag cacgccgtgc tgcacatcgt gccgaacatg aacccggacg gcgcggtgcg
     2581 cggcaacctg cgcaccaacg ccgccgggcg caacctcaac cgcgaatggc gcgcgcccga
     2641 ccccgtcgcc agccccgagg tcttcctggt gcgcgcggaa atggagcgca ccggctgcgc
     2701 gctcttcctc gacatccacg gcgacgagtc gctgccctac gtgttcttct ccaccgccga
     2761 ggaggtgcct ggcttcaccg ccgcggcggc ggccgcgcag gcgcgcttcg tcgagaccct
     2821 cctcatggtc agccccgact tccagacgca ggagggctac aagcccggcc gcttcggcga
     2881 ggaactgctg acgctggcga gcaagtgggt cgccaaccag ttcggctgcg tgtcgctgac
     2941 cctggagatg ccgttcaagg acaacatcat cctgcccgac ggccacgtcg gctggaacgg
     3001 tgcgcgcagc aagcgcctgg gcgcggccat gctcgatccg atcctgcgcc acctgcgtga
     3061 gggcggcgaa ggctgatcgg gaggggaggc ggtggcgagt ggacgtggcg gcgacgtgca
     3121 gctgcgtccc cgcttcacgg ccgcgaggcc gcggccggat tcaccgagcg gatcaggcct
     3181 tgtgcccgat ctcggcgagg atcgcggtcg cgcaggccac acccgagcgc accgcggatt
     3241 ccagcgtcgc ggggtagtcc gaatccaggt aatcgccggc cagccacagt ccgggcagcg
     3301 gggtgcggat gccggggcgg aagacgcctg gccgacacgc gaaagtggcg cgcttttcga
     3361 cgatgacctg cgaccactcc ggggctggca gacggcgacc gagctggcgt tcgagctggg
     3421 cgtgcaccgc gaggacgagc tcgtcgcgcg cgagcgcctc gtgcgggccg tgggcgctga
     3481 tcacgcacga gaagaggccc tccggcccgc cgagctggcc gcggtcgaag acccactgcg
     3541 ccggcccggc ggtgctgccc gaggccaggc cgatcatcgg ctcggggagc tgctggccgc
     3601 gtccgagggc gagatagacg gacacgatcg gctcgtgctc gagcgcgtcg atctgcgcca
     3661 ccaggcgctc gcaaccgccg acggaggcga gcagcgctcc ggcatggtag ggcgcggtgg
     3721 ccaccaccac gtgaggccag gggtggctgc cggggtcgcc gtcgaggcgg tagccgccgg
     3781 ccacacgttc gacggcctcg atcgcgttgc cggtgcgcag cctgccgcgg cgcacaccga
     3841 tgtagcgcgc cgccggcacc gggaagagct cggacaggtc ggtgcgcggc agcagcagct
     3901 cgctggcggc ggcacccgca ccgaggctgt cgcgcagcac gttcgcgaag acctgggccg
     3961 aggccgcggc ggccggggtg ttgagcgccg ccacgcacag cggctcccag atcagctggc
     4021 acagcgtcgg agtctggcgg gtctcggcga gcagggcgtc gaccgtcatc cccggatcgg
     4081 gcaggcggaa gccgcgtcgc ttcaaccagc gcatcaggcg cagcatcgcc aggcggtcgg
     4141 cccagtgcag cccctgcgca cgcagcaacc ccacggcgag gtggaaaggc gccggcaggg
     4201 acgcggcctt caggtgcagg cggccgggga cgtgcagggt cagcggcagg tgggcgagct
     4261 gcttgggcga cacgccggtg aggcgcagca ggcgggtaag ctcgctgtag gcgccgatca
     4321 ggatgtgctg gccgttgtcc acccgccagt ggtggtcctt gtgcaccacg cgggcgcgtc
     4381 cgcccatggt gtgcgagcgt tcgaacacgg tgacatgcac cccggcgcgg gccagctcga
     4441 ccccgcaggc gagcccggcg tagcccgcgc cgatgatggc gacgaccggc acgctcatgg
     4501 ccggaggttc cggctgccgt gccagctcga tcccgcgcgc tgggtgcgct tccctgcggg
     4561 cgtgctcatg cgcggaacca ggtcgtgccc gcgagccaca ccttgcgcag cggggtcagg
     4621 g
//
LOCUS       DAOQNN010000261         4618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11508_length_4618_cov_10.599825, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000261 DAOQNN010000000
VERSION     DAOQNN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11508_length_4618_cov_10.599825"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PKV97_12485"
     CDS             complement(<1..518)
                     /locus_tag="PKV97_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNB06743.1"
                     /translation="MPTIRATLHATLAASLLLALLPPAHAQRAGNIGGCDIRRGTLCT
                     NLNLNGANLEGVNLANSQFTRSDLSGANLRGATLNEANFSQAEMAGAKLDKASMLRAN
                     LRGARLTGASFKEADLRNAILQNADLHDADLTAAQLGGADLTNARAERSRFDGAELTR
                     SNLRAAKLAGAS"
     gene            complement(539..1036)
                     /locus_tag="PKV97_12490"
     CDS             complement(539..1036)
                     /locus_tag="PKV97_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB06744.1"
                     /translation="MKLKIAAAVAVLVLAVAGFFVFTQKPAAPDVAFSTLQGQSFRTA
                     DLRGKVVLVNFWATTCTTCIKEMPALKATQEKFEARGYRTVAVAMDYDPPAQVAAFVE
                     RSALPFTFVLDRDGSIARGFEGVRLTPTSYVLDKHGRIVQKILGEPDFDKLHGLLDQL
                     LAEPA"
     gene            1239..1679
                     /locus_tag="PKV97_12495"
     CDS             1239..1679
                     /locus_tag="PKV97_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNB06745.1"
                     /translation="MTSPPVFTWSDRFLLGHRTIDDTHREFVECVDALLRASDAEIPA
                     ALDAFARHAEAHFAQEDAWLSAADFPTGGDCHIDEHAKVMASVREVQELVAKGEVEIA
                     RELAQALMDWFPGHADYMDSALATWLVKKSHDGRPLVLRRKENL"
     gene            1841..3832
                     /locus_tag="PKV97_12500"
     CDS             1841..3832
                     /locus_tag="PKV97_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB06746.1"
                     /translation="MSALLNAPQREAIRYLDGPCLVLAGAGSGKTRVITHKIAHLINE
                     CGIGAANIAAITFTNKAAKEMQERVAHIMGGRVPGGLTVCTFHALGVRIIRQEAKHCG
                     LKPQFSILDASDTVQIVSDVAGDSDKGIAKQMQWQISSWKNAMITPEEAAQLADNEIA
                     SVAARLYKEYERTLRAYQAVDFDDLIALPVRLFDEHPEVRERWQNKLRYLLVDEYQDT
                     NRAQYRLLKLLSGVRGAFTAVGDDDQAIYAWRGADVENLKLLQQDYPKLKVIKLEQNY
                     RSSRRILEAANTVIANNAKLFDKRLWSEHGQGEQIVVTNCRDAEHEAEWVATKITAHK
                     FEHRTRFKDYAILYRGNHQARLIEQQLRNHRIPYVMSGGQSFFDKAEIRDLIAWLRLL
                     VNEDDDLAFIRAITTPRRGIGAATIEALGAYAGHRHTSLFTAVFEEGLTQHLNAKQLQ
                     GVQEFAAYINRLQYRAPREPAAQLLEDLLGAIRYEAWLFEHCDTREAESKWSNVRDFV
                     GWLGRKGEEDGKNLLELTQTIALMSMLDKEDPDFDGVQMATLHASKGLEFPHVFLVGV
                     EEGLLPHQSSIDEDKVEEERRLMYVGITRAQRSLNLTWCERRKSGKEFRTCEPSRFIA
                     EMGGDIKMNDRKTAQPVTKEEGKARLANLMAMFENRDKA"
     gene            complement(3971..4417)
                     /locus_tag="PKV97_12505"
     CDS             complement(3971..4417)
                     /locus_tag="PKV97_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB06747.1"
                     /translation="MSNPRPLRPARLAMPVALVAAALLAGCGDSGKVDEELRATLIQP
                     VARVEIQAVTITPGTRTGEQIYKSICAACHDAGAVGAPKTGDAAAWGPRLALGHDGLT
                     ASAIAGKNAMPPRGGGSDLTDTEVKRAVAYLANLAGAGYTEPPVEQ"
BASE COUNT          754 a         1556 c         1631 g          677 t
ORIGIN      
        1 aaggacgcgc ccgcgagctt ggcggcgcgc aggttgctgc gggtgagctc ggcgccgtcg
       61 aagcggctgc gctcggcgcg ggcgttggtc aggtcggcgc cgccgagctg ggcggcggtg
      121 agatcggcgt cgtgcaggtc ggcgttctgc aggatggcgt tgcgcaggtc ggcttccttg
      181 aagctggcgc cggtcaggcg tgcgccacgc aggttggcgc gcagcatcga ggccttgtcc
      241 agcttcgcgc ctgccatctc ggcctgggag aagttggcct cgttcaaggt ggcgccgcgc
      301 aggttggcgc cggacagatc cgagcgggtg aactgcgagt tggccagatt caccccttcg
      361 aggttcgccc cgttgaggtt gaggttggtg cacagcgtgc cgcggcggat gtcgcagccg
      421 ccgatgttgc cggcgcgctg ggcgtgggcg ggcgggagca gggcgagcag caggctggcc
      481 gcgagggtgg cgtgcagtgt ggcgcggatc gtgggcatgg ggtgcgtggg cttcccggtc
      541 aggcgggttc ggcgagaagc tggtcgagca ggccgtgcag cttgtcgaag tcgggttcgc
      601 cgaggatctt ctgcacgatc ctgccgtgct tgtcgaggac gtagctggtc ggggtcaggc
      661 gcacgccctc gaagccgcgc gcgatgctgc cgtcgcggtc gagcacgaag gtgaagggca
      721 gggcgctgcg ctcgacgaag gcggcgacct gggcgggcgg gtcgtagtcc atcgccaccg
      781 ccacggtgcg gtagccgcgc gcctcgaact tctcctgcgt cgccttcagc gccggcatct
      841 ccttgatgca ggtggtgcag gtcgtggccc agaagttcac cagcactacc ttgccgcgca
      901 ggtcggcggt gcggaagctc tgcccctgca gcgtggagaa ggccacgtcg ggggcggcgg
      961 gcttctgggt gaacacgaag aagccggcga cggcgaggac gaggaccgcg acggcggcgg
     1021 cgatcttgag cttcatggcg gggtccggaa cggttgaact gcgcggcggc aggcgcttta
     1081 cgggcgattt tgagcgagcg cccggggctt gtcaccttcg accggtcgga caggcgctgc
     1141 ctgcaaaggt gcccgtcttg ctagacttca agcatcgggg acgcgcgacc ggcgtccctg
     1201 cacccgtttt ccgacattcg atccgaggca agaccgcaat gaccagcccc cccgtgttta
     1261 cctggagtga ccgtttcctg ctcgggcacc gcacgatcga cgacacccac cgcgagttcg
     1321 tcgagtgcgt ggatgcgctg ctgcgcgcga gcgacgcgga gatccccgcc gcgctcgacg
     1381 ccttcgcccg ccacgccgag gcgcacttcg cgcaggagga cgcctggctc tccgcggccg
     1441 atttccccac cggtggcgac tgccacatcg acgagcacgc caaggtgatg gcgtcggtgc
     1501 gcgaggtgca ggagctcgtc gccaagggcg aggtggagat cgcgcgcgag ctcgcgcagg
     1561 cgctgatgga ctggttcccc ggccacgccg actacatgga ctcggcactc gccacctggc
     1621 tggtgaagaa gtcccacgat gggcgtccgc tggtgctgcg gcgcaaggaa aacctgtgag
     1681 cgcctcgcca ggcgggcgca ggcgacctcg ggcgagcgcc cggtgggcgc gcatccggcg
     1741 ccgtccggcc ggcactcgcc gggccgcacg cccgatcggc tagaatcgat ccccacgccg
     1801 cccggccggg cggctttgct tttctgcgat ccagctcacg atgtccgccc tcctcaacgc
     1861 gccccagcgc gaagcgatcc gctatctcga cggcccctgc ctggtgctgg ccggcgccgg
     1921 cagcggcaag acgcgggtga tcacgcacaa gatcgcgcac ctgatcaacg agtgcggcat
     1981 cggcgcggcc aacatcgccg cgatcacctt caccaacaag gccgccaagg agatgcagga
     2041 gcgcgtcgcc cacatcatgg gcgggcgcgt gccgggcggg ctcacggtct gcaccttcca
     2101 cgcgctcggc gtgcgcatca tccgccagga ggccaagcac tgcgggctga agccgcagtt
     2161 ctccatcctc gacgcctccg acaccgtgca gatcgtctcg gacgtcgccg gcgacagcga
     2221 caagggcatc gccaagcaga tgcagtggca gatctcgtcg tggaagaacg cgatgatcac
     2281 gcccgaggag gccgcgcagc tcgccgacaa cgagatcgcc tcggtcgccg ccaggctcta
     2341 caaggaatac gagcgcaccc tgcgcgccta ccaggcagtg gatttcgacg acctcatcgc
     2401 gctgccggtg cgcctgttcg acgagcaccc cgaggtgcgc gagcgctggc agaacaagct
     2461 gcgctacctg ctggtggacg agtaccagga caccaaccgc gcccagtacc ggctgctcaa
     2521 gctgctgtcg ggcgtgcgcg gcgccttcac cgcggtgggc gacgacgacc aggcgatcta
     2581 cgcctggcgc ggcgccgacg tcgagaacct gaagctgctg cagcaggact accccaagct
     2641 gaaggtgatc aagctcgagc agaactaccg ctcctcacgc cgcatcctcg aagccgccaa
     2701 caccgtcatc gccaacaacg cgaagctctt cgacaagcgc ctgtggtccg agcacggcca
     2761 gggcgagcag atcgtcgtca ccaactgccg cgacgccgag cacgaggccg agtgggtggc
     2821 gaccaagatc accgcgcaca agttcgagca ccgcacccgc ttcaaggact acgccatcct
     2881 ttaccgcggc aaccaccagg cccgcctcat cgagcagcag ctgcgcaacc accgcatccc
     2941 ctacgtgatg tcgggcgggc agagcttctt cgacaaggcc gagatccgcg acctcatcgc
     3001 ctggctgcgc ctgctggtga acgaggacga cgacctcgcc ttcatccgcg ccatcaccac
     3061 gccgcgccgc ggcatcggcg ccgccaccat cgaggcgctc ggcgcctacg ccgggcaccg
     3121 ccacaccagc ctgttcaccg cggtgttcga ggagggcctc acccagcacc tcaacgccaa
     3181 gcagctgcag ggcgtgcagg agttcgccgc ctacatcaac cgtctgcagt accgcgcccc
     3241 gcgcgagccc gccgcgcagc tgctggaaga cctgctcggc gcgatccgct acgaggcctg
     3301 gctgttcgag cactgcgaca cgcgcgaggc cgagtcgaag tggagcaacg tgcgcgactt
     3361 cgtcggctgg ctcggccgca agggcgagga ggacggcaag aacctgctcg agctcaccca
     3421 gaccatcgcg ctgatgtcca tgctcgacaa ggaagacccc gacttcgacg gcgtgcagat
     3481 ggccaccctg cacgcctcca agggcctgga gtttccgcat gtgttcctgg tcggcgtcga
     3541 ggagggcctg ctgccgcacc agagcagcat cgacgaggac aaggtcgagg aagagcgccg
     3601 cctgatgtac gtcggcatca cccgcgcgca acgcagcctc aacctgacct ggtgcgagcg
     3661 gcgcaagtcg ggcaaggagt tccgcacctg cgagccgagc cgcttcatcg ccgagatggg
     3721 cggcgacatc aagatgaacg accgcaagac cgcacagccg gtgacgaagg aagaaggcaa
     3781 ggcgcggctg gcgaacctga tggcgatgtt cgagaaccgc gacaaggctt gagcggccgc
     3841 tcgcttgtgg cgccccgaca ggaaagggcg cgataggggt ctgctgcagg ccggggcccc
     3901 aggcgcaaaa aaggcagccg cggaggctgc ctttcctgac ctggccgacg gctgcgacgc
     3961 agcccgcgga ttactgctcg accggcggct cggtatagcc ggcgccggcg aggttcgcga
     4021 ggtaggccac ggcgcgtttc acctcggtgt ccgtgaggtc gctgccgcca ccgcgcgggg
     4081 gcatggcgtt cttgccggcg atcgccgagg cggtcaggcc gtcgtggccg agcgccaggc
     4141 ggggtcccca ggccgcggcg tcgccggtct tcggcgcacc caccgcgccg gcgtcgtggc
     4201 aggcggcgca gatcgacttg tagatctgct cgccggtgcg ggttcccggc gtgatggtca
     4261 cggcctggat ctcgacacgg gcgaccggct ggatcagtgt ggcgcggagt tcttcatcga
     4321 ccttgcccga gtcgccacag ccggccagca gcgcggctgc gaccagggcg acgggcattg
     4381 cgaggcgggc gggccgcagg gggcgtgggt tcgacatgct ttgatcctcg gacggtggcg
     4441 caagacaggg tctaaagcgg ggattatagc gggctaaaaa ccattgtggc agcgcgccat
     4501 ggactttgcc gccgaagcgg gctatgcttc cgcgccttgt ccgggcgctt cgtccggaca
     4561 ccacgcgccc gtagctcagc tggatagagt actgccctcc gaaggcaggg gtcggacg
//
LOCUS       DAOQNN010000262         4590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11630_length_4590_cov_9.907166, whole genome shotgun sequence.
ACCESSION   DAOQNN010000262 DAOQNN010000000
VERSION     DAOQNN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11630_length_4590_cov_9.907166"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKV97_12510"
     CDS             <1..591
                     /locus_tag="PKV97_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB06748.1"
                     /translation="DENIMNSTRSTDFITPKSRTSRTFRNAVLGLAEHHPFARALVNS
                     GRLSVPSFLVDSRLNTADDAVFAGNMVPGAPMDDAPIESVGGEPWLLQMLGDRFQLLH
                     YVADAADLDAASVAALQSLAEGPIPVEPIVVAARGRAPAGLRTLIDAKGRIRERYDLQ
                     PGTTYLARPDQHVAARWRALDPARVRAAVARATCNA"
     gene            603..839
                     /locus_tag="PKV97_12515"
     CDS             603..839
                     /locus_tag="PKV97_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HNB06749.1"
                     /translation="MALNTQPNLFEPGKRYFRAFSPGDDFYEALIETHRDLSDEQSEM
                     VNARLILLLANHIGDIAVLREAMSIARAGVVAKD"
     gene            993..>1480
                     /locus_tag="PKV97_12520"
     CDS             993..>1480
                     /locus_tag="PKV97_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB06750.1"
                     /translation="MTPIVKKIHHVAYRCKDALETARWYEKHLGMKLVLSIAEDAVPS
                     TGEPDPYMHIFMDAGMGNVLAFFELPTRAPMGRDENTPAWTQHLAMEVDSMETLLATK
                     ARLESEGIQVVGPTDHALFQSIYFFDPNGHRLELACNTNRPGMLEALDKVKWDMLEEW
                     AQT"
     assembly_gap    1481..1580
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1581..1904
                     /locus_tag="PKV97_12525"
     CDS             <1581..1904
                     /locus_tag="PKV97_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNB06751.1"
                     /translation="PEAGVDMTFDFGQLVAHVARTRELEAGSIIGSGTVSNKQGDLWG
                     SSIDHGGVGYCCLAEVRTYETIEQGKPATSFMRDGDVVRIEMFDRQGRNVFGTIENRV
                     TARKG"
     gene            1910..2554
                     /gene="maiA"
                     /locus_tag="PKV97_12530"
     CDS             1910..2554
                     /gene="maiA"
                     /locus_tag="PKV97_12530"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HNB06752.1"
                     /translation="MKLYTYFRSSAAYRVRIALNLKGLDYEAVPVHLVRGGGEHRQPA
                     YLGLNPAGLVPALEDQGQVLTQSLAIIEYLEESHPQPALLPAAPLDRARVRAIAQAIA
                     CDIHPVNNLRVLQYLTRELDASEEQKNAWYRHWIGVGLQAVEAMLAGDARTGAFCHGD
                     TPGLADCCLVPQVFNARRFGCELAAMPTVLRIAETCAGLEAFQRAAPEAQPDAE"
     gene            2737..3921
                     /locus_tag="PKV97_12535"
     CDS             2737..3921
                     /locus_tag="PKV97_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family efflux MFS transporter"
                     /protein_id="HNB06753.1"
                     /translation="MSTAFVVLALSLLLGLQPVTTDLYLPALPSITQDLGATVAQAQL
                     TLSGLLLAFGLSQLVWGPISDRFGRRPVLLLGLASYVVAAVGSTLAGSMEALLLWRIA
                     QGAAMGASVMCARALVRDLYSPEIGARAISKGLTGLGVIACLSIPLGGVLAEFFGWRS
                     ALSALALFGAATLALIALRFEETLQRPDPDALRPATLVRTWITILRNPTFLAFALLAA
                     ATYGGLFTLLAASSFVFIRLMELSRIEYGALMFVIVLAYVGGTLLCRRLLVRFGVQRT
                     VAIGGVFSLAGGSLLGLGGLAGLEGVWPILLPALIYMVGHGIHQPCGQAGAVGPFPKA
                     AGAASAMSGFLMMVVAFAMGQWLGASMDGTPLPMTHGMWFWGVATAAVAWGLVGRVPR
                     QA"
     gene            complement(4039..>4590)
                     /locus_tag="PKV97_12540"
     CDS             complement(4039..>4590)
                     /locus_tag="PKV97_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNB06754.1"
                     /translation="EFDVFGFVRERTGNIMPGITPSNTHTTRDGRHITIGANGDAIFR
                     RLMRAMGRDDLAEDATLADNAGRDARREELYALIDAWVAQHDEAAVLATLAAAEVPAS
                     RIYSVADMFADPQFLAREMLHTVKLPDGRDCRMPGVVPKLSGTPGGSEWIGPALGAHT
                     DAVLAGLGYDEARIAALRAAGAI"
BASE COUNT          616 a         1592 c         1569 g          713 t
ORIGIN      
        1 gacgagaaca tcatgaactc gacgcgctcg accgacttca tcacgcccaa gagccgcacc
       61 agcaggacct tccgcaacgc ggtgctgggc ctggccgagc accatccctt cgcgcgcgcc
      121 ctggtcaact ccgggcgcct gtcggtgccg agcttcctcg tcgattcgcg gctcaacacc
      181 gccgacgatg cggtgttcgc cggcaacatg gtgccgggcg cgccgatgga cgacgccccg
      241 atcgagtccg tcggcggcga gccctggctg ctgcagatgc tcggcgaccg cttccagctg
      301 ctgcactacg tggccgacgc cgccgacctc gacgccgcca gcgtggccgc gctgcagtcg
      361 ctcgcggaag ggccgattcc ggtggagccg atcgtggtcg cggcgcgcgg ccgggcgccg
      421 gccggactgc gcacgctgat cgacgccaag ggccgcatcc gcgagcgcta cgacctgcag
      481 cccggcacca cctacctcgc ccgccccgac cagcacgtcg ccgcgcgctg gcgcgccctc
      541 gacccggccc gcgtgcgcgc cgccgtcgcc cgcgccacct gcaacgcctg aaggagaccg
      601 tcatggccct gaacacccag cccaacctgt tcgaacccgg caagcgctac ttccgtgcct
      661 tctcgcccgg cgacgatttc tacgaagccc tgatcgagac ccaccgtgat ctgagcgacg
      721 agcagagcga gatggtcaac gcccgcctga tcctgctgct cgccaaccac atcggcgaca
      781 tcgcggtgct gcgcgaggcg atgagcatcg ctcgcgcagg ggtggtcgca aaggactgat
      841 cgttacgcga gccggtcgat ggtggccgtg ctgcagacga cgtgcagcga gcgggatcgc
      901 tgcaatgatg atgcccggac cggagcgctc cgttatcttc ccgaaatgcc gcaacggagc
      961 ttcggcactg aaccaaggac gaggagaccg caatgacccc gatcgtgaag aagatccatc
     1021 acgtcgccta ccgctgcaag gacgcgctcg agaccgcgcg ctggtacgag aagcacctcg
     1081 gcatgaagct cgtgctgtcg atcgccgagg acgccgtgcc ctctaccggc gagcccgatc
     1141 cctacatgca catcttcatg gacgccggca tgggcaacgt gctcgccttc ttcgagctgc
     1201 ccacgcgtgc gccgatgggc cgcgacgaga acaccccggc gtggacccag cacctggcga
     1261 tggaggtcga ctcgatggag accctgctcg ccaccaaggc ccgcctcgag tccgagggca
     1321 tccaggtggt cggtcccacc gaccacgcgc tcttccagtc gatctacttc ttcgatccga
     1381 acggccaccg cctcgagctc gcctgcaaca cgaaccgccc gggcatgctg gaggcgctcg
     1441 acaaggtcaa gtgggacatg ctcgaggagt gggcgcagac nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn cccgaggccg gcgtcgacat gaccttcgac ttcggccagc
     1621 tcgtcgccca cgtcgccagg acgcgcgagc tcgaggcggg ctcgatcatc ggctcgggca
     1681 cggtctcgaa caagcagggc gacctgtggg gctcgtcgat cgatcacggc ggcgtcggct
     1741 actgctgcct cgccgaagta cgcacctacg agaccatcga gcagggcaag ccggccacgt
     1801 ccttcatgcg cgacggcgac gtcgtccgca tcgagatgtt cgaccgccag ggccgcaacg
     1861 tcttcggcac gatcgagaac cgggtgacgg cgcgcaaggg ctgaggcaag tgaagctcta
     1921 cacctacttc cgcagttcgg ccgcgtatcg cgttcgcatc gcactgaacc tcaagggcct
     1981 ggactacgag gccgtgccgg tgcacctggt gcgcggcggc ggcgagcacc gccagcccgc
     2041 ctacctgggc ctcaatcccg ccggcctggt gccggcgctg gaggaccagg gccaggtgct
     2101 gacccagtcg ctggcgatca tcgagtatct cgaggagagc catccgcagc cggcactgct
     2161 gccggccgcg ccgctcgacc gcgcccgcgt gcgtgcgatc gcgcaggcga tcgcctgcga
     2221 catccacccg gtgaataacc tgcgcgtgct gcagtacctc acgcgcgagc tcgatgccag
     2281 cgaagagcag aagaacgcct ggtaccggca ctggatcggc gtcggcctgc aggcggtgga
     2341 ggcgatgctc gccggcgatg cgcgtaccgg tgcgttctgc cacggtgata cgcccggcct
     2401 ggccgactgc tgcctggtgc cgcaggtctt caacgcccgc cgcttcggct gcgagctggc
     2461 ggcgatgccg acggtgctgc gcatcgcgga gacctgcgcc gggctggagg ctttccagcg
     2521 cgccgcgccc gaggcccagc ccgacgccga gtgagcggcg cagtctggct ttccggtctt
     2581 gcaggtgagc ggttgcgaat ggctgcgcgc gcaggcgagg gagaccgggg cgcgtgcgca
     2641 agtcgcgcag ggatgcccgg cgacgatctt tttttgccct tcgtcctgcc accggcggta
     2701 tcttccgcgg gcttggtccc cgttttcgga gtcccgatgt cgaccgcttt cgtcgtcctt
     2761 gccttgtcgc tgctgctcgg cctccagccg gtcaccaccg atctctatct gcccgcgctg
     2821 ccctcgatca cccaggacct gggcgccacg gtggcgcagg cgcagctcac gctgtccggc
     2881 ctgctgctcg ccttcggcct gtcgcagctg gtgtggggac cgatctcgga ccgcttcggg
     2941 cgccggccgg tgctcctgct cgggctggcg agctacgtcg tggcggcggt gggcagcacg
     3001 ctcgccgggt cgatggaggc gctgctgctg tggcggatcg cccagggcgc ggcgatgggg
     3061 gcgtcggtga tgtgcgcgcg tgcgctggtg cgcgacctct acagccccga gatcggggcg
     3121 cgcgcgatca gcaaggggct caccgggctg ggcgtgatcg cctgcctgag cattccgctc
     3181 ggcggcgtgc tcgccgaatt cttcggctgg cggagcgcct tgtcggcgct ggcgctgttc
     3241 ggtgcggcca ccttggcgct gatcgcgctg cgcttcgagg agaccctgca gcgccccgat
     3301 ccggatgcgc tgcgcccggc cacgctggtg cgcacctgga tcaccatcct gcgcaacccc
     3361 accttcctcg ccttcgcgct gctggctgcg gcgacctacg gcggcctgtt caccctgctg
     3421 gcggcctcgt ccttcgtgtt catccgtctg atggagctgt cgcgcatcga gtacggcgcg
     3481 ctgatgttcg tgatcgtgct cgcctacgtt ggcgggaccc tgttgtgccg ccgcctgctc
     3541 gtgcgcttcg gcgtgcagcg cacggtggcg atcggcggcg tgttctcgct cgcgggcggc
     3601 agcctgctcg ggctcggcgg gctggccggc ctggagggcg tgtggccgat cctgctcccc
     3661 gccttgatct acatggtcgg ccacggcatc caccagccct gcggccaggc gggcgcggtc
     3721 gggcccttcc cgaaggcggc gggcgccgcc tcggcgatga gcggcttcct gatgatggtg
     3781 gtggcgttcg cgatggggca gtggctgggc gcgagcatgg acgggacgcc gctgccgatg
     3841 acccacggca tgtggttctg gggcgtggcg acggccgcgg tggcctgggg gctggtcggc
     3901 cgggtgccgc gtcaggcttg aatgcatggg gcgggtggcg cgaagcgggg cggctccgat
     3961 gggaacggcg ctcgctccac cgccgcattc gcgccttgcg gcgctcctgc agggaggtcc
     4021 tgcatggagg tcgccggatc agatcgcgcc ggctgcgcgc agcgcggcga tgcgggcctc
     4081 gtcgtaaccc aggcccgcca acaccgcgtc ggtgtgcgcg cccagcgccg gcccgatcca
     4141 ttcgctgccg ccgggcgtgc ccgacagctt cggcaccacg cccggcatgc ggcagtcgcg
     4201 cccgtcgggc agcttcaccg tgtgcaacat ctcgcgcgcc aggaattgcg ggtcggcgaa
     4261 catgtcggcc accgagtaga tgcgcgaggc cggcacctcg gcggcggcca gcgtcgccag
     4321 caccgcggcc tcgtcgtgct gcgccaccca ggcgtcgatc agcgcgtaca actcctcgcg
     4381 gcgagcgtcg cggccggcgt tgtcagcgag cgtggcgtcc tcggcgaggt cgtcgcgccc
     4441 catcgcacgc atcaggcggc ggaagatcgc gtcgccgttg gcgccgatgg tgatgtggcg
     4501 gccgtcgcgg gtggtgtggg tgttggacgg cgtgatgccg ggcatgatgt tgccggtgcg
     4561 ctcgcgcacg aagccgaaca cgtcgaactc
//
LOCUS       DAOQNN010000263         4578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11670_length_4578_cov_8.734247, whole genome shotgun sequence.
ACCESSION   DAOQNN010000263 DAOQNN010000000
VERSION     DAOQNN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11670_length_4578_cov_8.734247"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="PKV97_12545"
     CDS             complement(<1..1521)
                     /locus_tag="PKV97_12545"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNB06755.1"
                     /translation="MKSHWCRLLLALGLWLAGAGSIGRAAEAAPRLPVDESAAERDAR
                     LAWWREARFGLFIHWGPASLSGREISWSRIGHPHDHPGLESTPAAEYDRLYERFDPVK
                     FDADAWMRLARDAGMKYVVFITKHHDGFSMWRTQLRPDYSIAATPFGRGGRDICAELA
                     AAAHKYGLKLGWYYSTRDWTHPDYLVGDNAKYDAFYRGQVRELLSQYGRVDLMWFDHV
                     AGNWRDYRFEELFDMMYRLQPGLVVNSRAAAFIRGTADQPSARVQTLVRGDFDTPEQR
                     IGVFQNDRPWESCVTLTECADGGGWSYRPDGRTRTFAECVRMLVNCATGDGNLLLNVG
                     PLPTGEIASGQQAVLRGMGAWLRRYGASIYGTRGGPYRNGDWGGATYRDRTVYLHVLK
                     PGVGRLTFPPLRARVLRATSLTGGSPVVHQTAAGLTVTLPRAASADTNRLDTIIRLEL
                     DAPVAAEFNEGRPLNVPEIATVTLEAPLDYQVVQRRTARAGRVVVSGRAPAGTDRVK"
     gene            complement(1652..2014)
                     /locus_tag="PKV97_12550"
     CDS             complement(1652..2014)
                     /locus_tag="PKV97_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06756.1"
                     /translation="MSHIPNLVSIHPYFKIKPGKADEFKASLPAFIAKTATEKLNLFY
                     DFTIHDDVVLCREGYVGAEGLLTHLDGVGPLLGPLMTLADLVRVEVHGPAAELEKLKG
                     PLAALNPTWHTRLLGVER"
     gene            complement(2341..2751)
                     /locus_tag="PKV97_12555"
     CDS             complement(2341..2751)
                     /locus_tag="PKV97_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06757.1"
                     /translation="MAVTPEEIGLHGCWQVIAVERQSQDLTNLAESPSLEIGCYATSL
                     ALGQYDDDAILALIRNHWSAIENGTHYRRDVSLGEDACRASHRGGAAVLASLRNLANG
                     IYELEKERDRTRVDSLKSWCQQQTFSTVWPILNR"
     gene            complement(2789..4087)
                     /locus_tag="PKV97_12560"
     CDS             complement(2789..4087)
                     /locus_tag="PKV97_12560"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HNB06758.1"
                     /translation="MVRLLRDEERERFNRHLENEHYLESSTLVGRWLRYVAEVDGQWV
                     ALLTFSAAALHIKARERWIGWSPRQRARRLGFVVNNSRFLVLPERQRYPNLASRVLGL
                     CLRRLSHDWQEQWGHPVLVVESFVDESQYRGTCYKACGFEAVGPTEGFGRASRDFYAA
                     HGQPKQLYLRQLRPGLREKLRQAKWPEELARHEANLAGPCPFRAPALENLLERMAALR
                     DERRGHGLRHQQRFVLACAAVCTVMGACGYRAFENTCMKFTQRQLRALGCTPDEADGR
                     YYPPSDSTFQRVLNRVDSAALATLIGQWLAQQEVGALAQLAVDGKVLRGSGRHDGKPL
                     QLLSAVTHHLRLTLDQVAIEAKSNEIPALKPLLKKLDLPPNTLITADAMHCQQESARC
                     ITQELGGDYLFGLKGNQSGILEQAQHLLTQQGFPPTGSMG"
BASE COUNT          774 a         1533 c         1481 g          790 t
ORIGIN      
        1 cttcacccgg tccgtccccg ccggcgcccg accgctcacc accacgcgcc cggctctggc
       61 ggtccggcgc tggacgacct gataatcgag cggcgcctcg agcgtcaccg tcgcgatctc
      121 gggaacgttg aggggccggc cttcgttgaa ttccgccgcg accggcgcgt cgagctcgag
      181 ccggatgatg gtgtcgagcc ggttcgtatc cgctgacgcc gcgcggggca gcgtcaccgt
      241 caaacctgcg gcagtctggt gcaccaccgg cgaaccgccg gtcaggctcg tggcccgcag
      301 cacccgtgcg cgcagcggcg gaaaggtcag tcggcccacg cccggtttca gcacgtgcag
      361 gtacaccgtg cggtcgcgat acgtggcgcc gccccaatcg ccgttccggt aaggaccgcc
      421 gcgggtgccg taaatgctcg cgccgtaccg ccgcagccag gcccccatcc cgcgcaacac
      481 ggcctgctgg ccggaagcga tctcgcccgt cggcaacgga ccgacattca ggagcagatt
      541 gccgtcgccc gtcgcgcagt tcaccagcat ccgcacgcat tcggcgaagg tccgcgtccg
      601 cccgtcgggc cgatacgacc agccgccgcc gtcggcgcat tcggtcagcg tgacgcacga
      661 ctcccatgga cgatcgttct ggaagacgcc gatccgctgt tccggggtgt cgaagtcgcc
      721 gcgcacgagt gtctgcaccc gggccgacgg ttgatcggcg gtgccgcgaa tgaaggcggc
      781 ggcgcggctg ttcaccacca ggccgggctg cagccggtac atcatgtcga acaactcctc
      841 gaaccggtaa tcgcgccagt tcccggccac gtgatcgaac cacatcaggt ccacgcggcc
      901 atactgggac aacagctccc ggacctggcc gcggtagaac gcgtcgtact tcgcgttgtc
      961 gcccaccagg taatcgggat gggtccagtc gcgggtcgag tagtaccagc cgagcttgag
     1021 gccgtacttg tgcgccgccg cggccagctc ggcacagatg tcccggccgc cgcggccgaa
     1081 cggcgtcgcc gcgatgctgt agtccggccg cagctgggtg cgccacatcg agaaaccgtc
     1141 gtgatgcttg gtgatgaaca cgacgtattt catgccggca tcgcgcgcca gccgcatcca
     1201 ggcgtcggcg tcgaacttga ccggatcgaa ccgctcgtac agccggtcgt actcggctgc
     1261 cggcgtggac tccagtccgg ggtgatcgtg cggatgcccg atgcgcgacc agctgatttc
     1321 gcgtccgctc aggctggccg ggccccagtg gatgaacagg ccgaagcgcg cctcgcgcca
     1381 ccaggccagc cgggcgtccc gttcggccgc gctctcgtca accggcagcc ggggcgccgc
     1441 ttcggccgcc cggccgattg aacccgcacc cgccagccac agccccaacg cgagcaggag
     1501 ccggcaccaa tgagatttca tgaagggaga aggagtcccg gcggccggcc gtggaaatcc
     1561 ggcgccgccg gggagaatga acgaagcaat gcccgcccgg ggcggccggt tgcaggtggt
     1621 ccggcccggc ccggtgggac gtgagttcgg atcaacgttc cacacccagc agccgggtgt
     1681 gccaggtcgg attcaacgcg gcgagcgggc ccttgagctt ctcgagttcc gccgccggcc
     1741 cgtgcacttc cacccgcacg agatccgcga gcgtcatgag tggtccgagc aacggtccga
     1801 cgccatccag atgcgtcagg agaccttcgg cgccgacata gccttcgcgg cagaggacga
     1861 cgtcatcgtg gatggtgaag tcgtaaaaca ggttcagctt ttcggtcgcg gtcttggcga
     1921 tgaaggcggg cagcgacgct ttgaattcgt cggccttccc gggcttgatc ttgaagtaag
     1981 ggtggatgct caccaggttc ggaatgtggc tcatggcggt gaattcgagt tggcgttgaa
     2041 ggatcgcgga cgttggtggt tgcggccgac gctaccaacc ggacgcccct gagtccatcg
     2101 gcaacgcgac cgcaccgctc gcggcaccac ggctgattca tctggttttg gggagggagg
     2161 gctgtagcct tgcgagacgg gctcgtagcg caacctcgtt cgggagtcgg cgtgccgtgg
     2221 gtttttgagt cctttacggc gcccggcact cccgcgggtc ctattgggtc cagttccggg
     2281 gtggcgcggc caagtcactg gccgccaccc tggagctcct tgggttttgc gaggcgaggt
     2341 tcaccgattg aggatcggcc agacggtcga gaaggtctgt tgttggcacc aggatttgag
     2401 cgagtcgacg cgagtgcgat cacgctcctt ttccagttcg tagatgccat tggctaagtt
     2461 acgcaggctg gccaagacgg cggccccacc gcgatgcgag gctcggcagg catcctctcc
     2521 caggctgacg tctcgccggt aatgcgtgcc gttctcgatg gccgaccagt ggttgcggat
     2581 cagggccagg atcgcgtcgt catcgtattg ccccagcgcc aggctggtgg cgtagcagcc
     2641 gatttccagc gagggagatt cggcgaggtt ggtcaagtcc tgcgactgac gttccaccgc
     2701 gatgacctgc cagcagccgt gcaggccgat ttcctccggg gtgacggcca cgcgcgcgat
     2761 gcggcgtcgg tcgaggcgcc cgtgaccctt atcccatcga cccagtgggg ggaaaaccct
     2821 gctgagtcag caggtgttgg gcttgctcga ggatgccgct ctggttgcct ttgagtccga
     2881 agagataatc gccgcccagt tcctgggtga tgcagcgagc gctctcctgc tggcagtgca
     2941 tcgcatcggc cgtgatgagc gtgttgggcg gcagatccag cttcttgagc agcggtttca
     3001 aggctgggat ctcgttgctc ttcgcttcaa tggcgacctg atcgagcgtc agccgcagat
     3061 gatgcgtgac ggccgagagc aactggagcg gcttgccgtc gtgccgtccg ctgccgcgca
     3121 acaccttgcc atcgacggcc aattgcgcca gggcgccgac ttcctgttgg gcgagccact
     3181 gtccgatgag cgtggccagc gcggccgagt cgacgcggtt gagcacgcgc tggaaggtcg
     3241 agtcactcgg cgggtagtaa cggccgtccg cttcatcggg ggtgcaaccc aaggcacgga
     3301 gttggcgctg ggtgaacttc atgcaagtgt tctcgaaggc ccgataacca cacgcaccca
     3361 tcacggtgca cacggcggcg caggccagca cgaagcgctg ttggtggcgc aagccgtgcc
     3421 cgcgacgttc atcgcgcagc gccgccatac gttcgagcag gttctccagc gctggagccc
     3481 ggaacgggca cggtccagcc aggttggctt catgccgggc gagttcttca ggccacttgg
     3541 cctgacgcaa cttctcacgc aagccgggcc gcagctgccg caggtacagt tgcttcggtt
     3601 ggccgtgcgc tgcatagaaa tcacggctcg cacgcccaaa tccctccgtc ggaccaaccg
     3661 cctcgaagcc gcaggccttg taacaggtgc cgcggtattg gctctcgtcg acgaaactct
     3721 cgaccaccag aaccggatgg ccccactgct cttgccaatc gtgactgagt cgccgcaggc
     3781 acaggcccag gacgcgggag gccaggttgg gatagcgctg acgctccggc agcaccagaa
     3841 accggctgtt gttcaccaca aaaccaagtc gacgcgcccg ttgccgcgga ctccagccaa
     3901 tccaccgctc acgcgctttg atgtgcagag ccgcggcgct gaaggtcagc aacgcaaccc
     3961 actgcccatc gacctcagcc acataccgca gccaccggcc gaccagcgtg ctgctctcaa
     4021 ggtagtgttc gttctcaagg tgacgattaa atcgctcacg ctcctcgtcc cgaagcaggc
     4081 gcaccacgac acgcttgaga acgtcggact caggctgcga acacgcgtta accatgcgcc
     4141 cacggtaaat cgaagcgcgc cgcgcgtcca gtcatttccc gcccctctcc agcccctatt
     4201 catcccattc acccagatga atcagccctg actcgcaagc ttctgcatcg caattgcccg
     4261 attgccgcgc acgcaccggc cgcgatggcg ctcggcgccc aatccgccct catcacttcc
     4321 aatccgggtc atccggccta gtagacaatg cccgaacctc gcgctaatcg tggcgtcatg
     4381 ggtcagggag actttggatg gcaacggacg atcgacggat tcgcacggtc gcttgcgccc
     4441 gcaacactcg cgttcctgta tcccgatcga tgtcagatct gccgtcgagg gcgagcccgg
     4501 gctgatgatg gctacgtatg cacgaactgc cgggaaggct caggcggcgt ccggttcatc
     4561 cggccaccct tctgccat
//
LOCUS       DAOQNN010000264         4551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11787_length_4551_cov_11.262900, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000264 DAOQNN010000000
VERSION     DAOQNN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11787_length_4551_cov_11.262900"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1835
                     /locus_tag="PKV97_12565"
     CDS             <1..1835
                     /locus_tag="PKV97_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNB06759.1"
                     /translation="NAAEGGRSGDNMNLRGFYTFGDMYLDGIRDTAQYNRETFNLEQI
                     DVLRGSAAMLFGRGQAGGVINQVTKTPLLGNRGRITGSVGIDDYREVTGDFNKELGDT
                     TAVRLNVMKRDEGSWRSNPTDGAEPEIHREGAGLSLVTGLYTDNEFTFNHYYLKTRDN
                     PDYGISFDPATKRPTKNFSADTFWGTNNTFDDSDTSMTSLVHQYKLSSQAEIRTQLRH
                     ASYERVYWAQAPERINPPSFDGSTGSPKVRQTDTENLTLQSDLTARAELLGMKHELLA
                     GVEYLKEDSSRWGLRDLDPGAGRFYRPGAFTSAPAITYQGDTLAFYAQDTIEFVPNWK
                     LTLGARHDSLDAEYSSLNSPQLDFSETSLRTGLSWHPNAYTHYYISYSDSFSPTADLY
                     QLSGGSYPAERSKVTELGAKWMLMEGDLALRAALYRADKEWERNTDLESTAAILTKKR
                     RTDGLELEVAGRVTPKWEVFSGFSLMDAEILEVAPGADARLEGQRPRNTPKLTFNLWS
                     TYALGGGWKIGGGVEAKSDRYAYVPTAANANSNFVNGKFSPNTAPGYARWDAMLAYEQ
                     KSWAARLNVKNIFDRVYYDAVYDQGGFAIPGSGRAIILTGEYKF"
     gene            1894..3072
                     /locus_tag="PKV97_12570"
     CDS             1894..3072
                     /locus_tag="PKV97_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HNB06760.1"
                     /translation="MNKRDFLRTLGGGLSLALLPRTLFAATTPGELPAIRLIAAWRSL
                     HDLGRDGASRGAKAEGSTDYVGILATDWQQGLARIETAVAVPDRVHGLLPDRDGGFLV
                     CANRPGPWLMRCAADGEVLARQVLEDEKSGRTLNGHAAFDPAGEWLYTTESETGSTQG
                     WIAVRRRDSLRKEAEFSTRGIEPHELLFDAEGKLVVANGGILRAAGDRKRDLERMDSS
                     LVRLDPRSGERLGQWWLKDKRLSLRHLALGSAPAAGGPALVGIALQAEHDDPAERAAA
                     PILAVWNGDSLAIPSRETVGAGYCGDIATGMDGGFYLSAERSHRVARWHPAAPARLEI
                     IAELNKAGALAELPLDGTHASLIGSPRGLGFWHPQHDPMLLRWPVEIAPDNHWTLVGA
                     "
     gene            complement(3311..3994)
                     /locus_tag="PKV97_12575"
     CDS             complement(3311..3994)
                     /locus_tag="PKV97_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06761.1"
                     /translation="MSQRKSGLRPIVLASLFALCSPPALAERPMAVDDAGTLERGGAK
                     LEFGWSRDDAVRGYEGAAGFGPIDNVEVEVGYGRGKDRDADPDAKIRAVGAAVKWVPL
                     QSERGLSAGLKYEYGRERVSGEDSSRVDALAGLATWAFEQGPRVHVNLGREWARDDED
                     LDFWGIGLDVPLGEQLDFVVETFGAEHSGPDRQVGLRYTIAEGVKISGAVGRGNDRNI
                     ANVGVAWEF"
     gene            complement(4363..>4551)
                     /locus_tag="PKV97_12580"
     CDS             complement(4363..>4551)
                     /locus_tag="PKV97_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06762.1"
                     /translation="NPLAPSSAGVTEKFTSSEQSGSMVSVFSIPRGEVHEQEMLQEGQ
                     ALQGMSAARKVSRNPARC"
BASE COUNT          820 a         1654 c         1427 g          650 t
ORIGIN      
        1 tcaacgccgc cgagggcggc cgctcgggcg acaacatgaa cctgcgcggc ttctacacct
       61 tcggcgacat gtacctcgat ggcatccgcg acaccgcgca atacaaccgc gagaccttca
      121 acctcgaaca gatcgacgtg ctgcgcggct cggccgcgat gctgttcggc cgcggccagg
      181 ccggcggcgt catcaaccag gtcaccaaga cgccgctgct cggcaaccgc ggcaggatca
      241 ccggcagcgt cggcatcgac gactatcggg aagtgactgg cgacttcaac aaggagcttg
      301 gcgacaccac cgccgtgcgt ctgaacgtga tgaagcgcga cgaagggagc tggcgcagca
      361 acccgaccga tggcgccgaa ccggagatcc accgcgaggg cgccggcctg agcctggtta
      421 ccggcctgta caccgacaac gagttcacct tcaaccatta ctacctgaag acgcgcgaca
      481 acccggacta cggcatctcc tttgatccgg cgaccaagcg cccgacgaag aacttctccg
      541 ccgacacctt ctggggcacg aacaacacct tcgacgacag cgacacctcg atgacttcgc
      601 tggtgcacca gtacaagctg tcctcgcagg ccgagatccg cacccagctg cgccatgcga
      661 gctacgagcg cgtgtactgg gcgcaggcac ccgaacgcat caacccgccg agcttcgatg
      721 gcagcaccgg cagcccgaag gtgcgccaga ccgacaccga gaacctgacc ctgcagtccg
      781 acctcaccgc acgtgccgag ctcctcggca tgaagcacga gctgctcgcc ggcgtcgagt
      841 acctgaagga agactcgagc cgctgggggc tgcgggatct ggatcccggt gcaggccgct
      901 tctatcgtcc gggcgccttc acctcggcgc cggcgatcac ctaccagggc gacacgctcg
      961 ccttctacgc ccaggacacg atcgagttcg tgccgaactg gaagctgacc ctgggtgcaa
     1021 ggcatgactc gctcgacgcc gagtattcca gcctcaactc gccgcagctc gacttcagcg
     1081 agaccagcct gcgcaccggc ctgtcctggc acccgaacgc atacacccac tactacatca
     1141 gctacagcga ctccttcagt ccgaccgccg acctctacca gctctccggc ggcagctacc
     1201 ccgccgagcg cagcaaggtc accgaactcg gcgccaagtg gatgctgatg gagggcgacc
     1261 tcgccctgcg cgccgcgttg taccgcgccg acaaggaatg ggagcgcaac accgacctcg
     1321 agtccaccgc cgcaatcctg acgaagaagc gccggaccga cggtctggag ctcgaggtcg
     1381 ccggtcgcgt caccccgaaa tgggaggtct tcagcggctt ctcgctgatg gacgccgaga
     1441 tcctcgaagt cgccccgggc gcagacgccc gcctcgaagg ccagcgcccg cgcaacacgc
     1501 ccaagctcac cttcaacctg tggtcgacct acgcgctcgg cggcggctgg aagatcggcg
     1561 gcggcgtcga ggccaagagc gaccgctacg cctacgtgcc gacggctgcc aacgcgaaca
     1621 gcaacttcgt caacggcaag ttctcgccca acacggcacc cggctacgcg cgctgggacg
     1681 cgatgctggc ctacgagcag aagagctggg ccgcgcgcct caacgtcaag aacattttcg
     1741 accgggtgta ctacgacgcg gtgtatgacc agggcggctt cgccatcccc ggcagcggcc
     1801 gcgcgatcat cctgaccggc gaatacaagt tctgatccga gcctatcgat cggcgggccc
     1861 ctgcgggccc gtcgcttcca ctggaacatc ccgatgaaca aacgcgactt cctgcgcaca
     1921 ctgggcggcg gcctctcgct cgccctgctg ccccgcaccc tgttcgccgc caccacgccc
     1981 ggcgaactcc ccgcaatccg cctgatcgcc gcctggcgca gcctgcacga cctcggccgc
     2041 gatggcgcct cgcgcggcgc gaaggcggag ggcagcaccg actacgtcgg catcctggcc
     2101 accgactggc agcaaggcct cgcccgcatc gagaccgcgg tcgcggttcc cgatcgggtc
     2161 cacggcctgc tgcccgaccg cgacggcggt ttcctggtgt gcgccaaccg tcccggcccc
     2221 tggctgatgc gctgcgccgc cgacggcgaa gtgctcgccc gccaggtcct cgaggacgag
     2281 aagagcgggc gtacgctcaa tggtcacgcg gccttcgacc ccgcgggcga gtggctgtac
     2341 accaccgaat ccgagaccgg ctccacccag ggctggatcg ccgtgcgccg gcgcgacagc
     2401 ctgcgcaagg aggcggagtt ctccacccgc gggatcgagc cgcacgagct gctgttcgac
     2461 gccgagggca agctggtggt cgccaacggc ggcatcctgc gcgccgcggg cgaccgcaag
     2521 cgcgacctcg agcgcatgga ctcctcgctg gtgcggctcg atccgcgcag cggcgagcgc
     2581 ctgggccagt ggtggctgaa ggacaagcgc ctgagcctgc gccacctcgc cctcggctcc
     2641 gcgccggcag cgggcggccc tgcgctggta ggtatcgcac tgcaggccga gcacgacgac
     2701 cctgccgagc gcgccgccgc ccccatcctc gcggtgtgga atggcgacag cctggcaatc
     2761 cccagccgcg agaccgtcgg cgcgggctac tgcggcgaca tcgccaccgg catggacggc
     2821 ggcttctacc tctccgccga gcgcagccat cgggtagcgc gctggcatcc ggcggccccc
     2881 gcgcgcctgg agatcatcgc cgagctgaac aaggcgggcg cgctggccga actcccgctc
     2941 gacggcaccc acgccagcct gatcggctca ccgcgcggcc tcggcttctg gcacccgcag
     3001 cacgatccga tgctgctgcg ctggccggtg gagatcgcgc cggacaacca ctggacgctg
     3061 gtcggagcct gaaccccgcc acccgcctgg atcgccgtat cgcgactcgc gtcgctccta
     3121 caggaagctc cgaagcgggg gttttctgca ggagcgacgc gcgtcgcgac atggagcgga
     3181 gaattcgccc cgacgcgttt cccgcttacg ggaaacgacc gccacgacga cccgcggctc
     3241 tcgagaaaac gcgctacaag ttggtatttt ctttgcgaac ccattccacc gcgggcgggg
     3301 gatgggacgc tcagaactcc caggccacgc ccacgttggc gatgttgcga tcgttgccgc
     3361 gaccgaccgc gccagagatc ttcacgccct cggcgatcgt gtagcgcaga ccgacctggc
     3421 gatccgggcc ggaatgctcc gcaccgaagg tctcgacgac gaagtcgagc tgctcgccga
     3481 gcggcacgtc gaggccgatg ccccagaagt cgaggtcctc gtcgtcgcgc gcccactcgc
     3541 ggccgaggtt cacatgcacc cgcgggccct gctcgaacgc ccaggtcgcc aggccggcga
     3601 gcgcgtcgac acgcgaggag tcctcgccgc tgacgcgctc gcgcccatac tcgtatttca
     3661 ggccagccga aagcccgcgc tcggactgca atggcaccca tttgaccgcc gcgccgacgg
     3721 cgcggatctt cgcgtctgga tcggcgtcac gatccttccc gcggccatag cccacctcca
     3781 cttcgacatt gtcgatcgga ccgaaaccgg ccgcgccctc gtagccgcgc accgcgtcgt
     3841 cgcggctcca gccgaactcg agcttggcgc cgccgcgctc gagcgtgccg gcatcatcga
     3901 ccgccatcgg gcgttcggcg agagccggag gggaacacaa ggcgaacagc gaggccagca
     3961 cgatcgggcg cagcccggac ttgcgttgag acatgacgat tcctttgcag ggagaggtgg
     4021 acaaagacgg gccgcccaca cgagcggccc gaaaagggat gcgcaactcc gaacgaccgg
     4081 ggctacgagg tgatcgctgg acaagaccca cgccgtcgat gcggcaggga cgcccgacac
     4141 tcgtgcctcg gacgtgcccg catgcgccgg gaggggcttc ttactcgagc acctggccag
     4201 acacggaaag ggcggtgcgg ccatgttcga gcaccgcatc gcgatcgagc ttcccggaat
     4261 attcggcccc cgcctcaatg cagccgatga tcgcggtcgc cgggcctggg gatgggttaa
     4321 aaaatattaa ccgcaacaac aatcattacc gtttgataat catcaacagc gcgcgggatt
     4381 ccggcttact ttgcgcgccg cggacatccc ttgcagggct tgtccttcct gcagcatttc
     4441 ttgctcatgg acttctcctc gtggaatgga aaagacggac accatcgaac cggactgctc
     4501 agagcttgtg aacttttcgg tcacgcccgc cgaggagggc gcgagtgggt t
//
LOCUS       DAOQNN010000265         4550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11792_length_4550_cov_6.313014, whole genome shotgun sequence.
ACCESSION   DAOQNN010000265 DAOQNN010000000
VERSION     DAOQNN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11792_length_4550_cov_6.313014"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..351
                     /locus_tag="PKV97_12585"
     CDS             115..351
                     /locus_tag="PKV97_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06763.1"
                     /translation="MSQTINEQMKLRRERQLQRIERANPDHFRFPREMPRQQRRIPFV
                     ADRRELVELRDAVIGLVAFVGLVAALVTAPWWFP"
     gene            596..1387
                     /locus_tag="PKV97_12590"
     CDS             596..1387
                     /locus_tag="PKV97_12590"
                     /inference="COORDINATES: protein motif:HMM:NF024096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HNB06764.1"
                     /translation="MSNADYHAHPALSKSQLADFMVCPANYFGLHLAPDRPARQETSG
                     QRAGTLLHTLVLEPDTFAERYAIGPDVSRAAKEWKTFAAALPADVTALKPDEYDEGLK
                     QAESLHRHADIADLLSSGEAETSIFWTDPITGLPCRCRPDWLHETPEGWIVLDLKTGP
                     ADPRTFGLQVARMSYDVQDAFYSEGIQIATGKPVIAFLFGVVETAPPFLSMCGLINDQ
                     GRESGKRKTRKALDAFARCKANNEWPGYEGVQLLTLPTYALDYQD"
     gene            1393..2523
                     /locus_tag="PKV97_12595"
     CDS             1393..2523
                     /locus_tag="PKV97_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06765.1"
                     /translation="MSQVTSLADIKAGKSNAVSSFNPDAWHAMQARDDQLIRDSVLHG
                     YAGKEYVYSFKIQGTQVTGVSVVGARELASHYGGIKHRIVASVDKTGSLFVFKSFEPL
                     AVQANIIPQLAGEEDFYEVVIEITDIKTGNSIQVRKKETKQEKRRDGSAYDRPHYDVI
                     AESKAYRNGVLSIIPQNVIKEFEERALKAGHAGETKTLDQLRSGALAFCAKNGIAVER
                     QSIANLTYAELQGLSASAANGVEAFRESAEALGLIRKAAAPAPALTEQQPVTMPPLQT
                     KTETPAYVDRETGEIQEPQRQQAEQSSGPGFADAHAAVRSGDFDLARDIARSLPTAQR
                     QQIETAIANLSAPPAEESENPAPTQQAPAARRSRGQAGLGLE"
     gene            2568..4421
                     /locus_tag="PKV97_12600"
     CDS             2568..4421
                     /locus_tag="PKV97_12600"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB06766.1"
                     /translation="MKIHSIHVEGFLGARQVDIALVEPITLIAGQNGAGKSSIANAIA
                     LALNAELGRVDVKKDAARLVTEGATAACVQIQTDSADFEVHITKAGKITDHAAGRKPH
                     PALPFALDASRFSRLPDAERRTLLFNLLGIETTPEAIAERLARMKCDADKVKAIGPAL
                     RAGFVAGEKHARELASQARGKWVAVTGEKAYGDKKAEGWEPPPAAALAGDPAALAENA
                     SARANELGEQIAALQQELGAARATREAAQQRAVRIERLRDQAGMVERIKGRLATAEEN
                     VASCKAQFAGAGSDDPKAPGSYLLRGLASALNEFVALTGESHGVVGYHLNGDVARWGE
                     FESLINRSAAHLGEYRKLHGDPDATPADPVRLAELRHALQTAESGAANARRDLAAAEA
                     AGAELAELEKAAPLEAPKIEDIETRLADARRRHADWQADVQKYLEAAGAAERRAQQIA
                     DAARHHADVQQWLAIADALSPDGIQAAMLADALDPINARLAENAALAQWPAPKITADM
                     AIVVDGRAIALLSESERWRTDALLTVSLGQLSGLRFAVLDRFDCLDMTGREDLLFWLS
                     DLAEAQQIDTVVVLGTLKAAPPAGALPPHIEAQWIERGALIPASQQTLEAA"
BASE COUNT          820 a         1662 c         1458 g          610 t
ORIGIN      
        1 cgttcgcatg aacggcgtcg cgctgatcgg gattgtgatt gtcgtctggc tcggggccgg
       61 actcctggtc gcgcgcctct ttcggatcaa ccggcatgac aaggacgacg aattatgagc
      121 cagaccatca acgagcagat gaagctcagg cgcgaacgtc agttgcagcg catcgagcgc
      181 gcgaaccccg accactttcg tttcccgcgc gagatgccgc gccagcagcg gcgcatcccc
      241 ttcgtcgccg accggcgaga gctggtcgag ctgcgcgacg cggtgatcgg cttggtcgcc
      301 ttcgtcggcc tcgtggccgc cctcgttacc gcgccgtggt ggttcccgtg accacgattc
      361 agccagcgcg gaccgtcttc ccgtcgcacg tgacacgcca gcttgcctgg cgccgggttg
      421 cactcacggt gccgttcgat gtgtggatcg agcgcgagta gatccagttc gcgcgccgca
      481 acggctaccg cacccgcaac ggcgccgcac aggcgcattt cgatcgatac atggagacct
      541 tgcaggcatg aatgccccgg aacgcatcaa cctcgagccc cgcgtcgtgc gcggcatgag
      601 caacgccgat taccacgcgc atccggctct cagcaaaagc cagctcgccg acttcatggt
      661 ctgcccggcc aattacttcg ggctgcacct cgcgcccgat cgccccgcgc gccaggaaac
      721 cagcggccag cgcgccggaa ccttgctgca caccctggta ctcgagcccg acactttcgc
      781 cgagcgctac gccatcggcc cggacgtgtc gcgcgcggcc aaagagtgga agaccttcgc
      841 cgccgccctg ccggccgacg tcaccgcgct caaacccgac gagtacgacg aaggcctcaa
      901 gcaggccgaa agcctgcacc ggcacgccga catcgccgac ctgctgtcga gcggggaggc
      961 cgagacctcg atcttctgga cggacccgat caccggcctg ccctgccgct gtcggcccga
     1021 ctggctgcac gaaacgcccg agggctggat cgtgctcgat ctgaaaaccg gcccagccga
     1081 tccgcgcacg ttcgggctgc aggtcgcgcg aatgtcctac gacgtccagg acgcgttcta
     1141 ttcagaaggg attcagatcg ccaccggcaa gcccgtgatt gcctttctct tcggggtggt
     1201 cgagaccgcg cccccgttcc tttccatgtg cggcctgatc aacgaccagg gccgcgaatc
     1261 tggcaagcgc aaaacgcgca aggcgcttga cgccttcgcg cgctgcaagg ccaacaacga
     1321 gtggcccggc tacgagggcg tgcagctact caccctcccg acctacgccc tcgactacca
     1381 ggactgacca ccatgagcca agtcaccagc ctcgccgaca tcaaagccgg caaatccaac
     1441 gccgtctcca gcttcaaccc ggacgcctgg catgccatgc aggcgcgcga cgatcagctg
     1501 atccgcgact ccgtgctgca cggctatgcc ggcaaggagt acgtgtactc gttcaagatc
     1561 cagggcaccc aggtcaccgg cgtgtccgtc gtcggcgccc gggagctcgc ctcgcactat
     1621 ggcggaatca agcaccgcat cgtcgccagc gtcgacaaga ccggctcgct tttcgtgttc
     1681 aagagcttcg agccgctcgc ggtgcaggcc aacatcatcc cgcagcttgc cggtgaggaa
     1741 gacttctacg aggtcgtgat cgagatcacc gacatcaaga ccggcaacag catccaggtg
     1801 cgcaagaaag agaccaagca ggagaagcgg cgcgacggca gcgcctacga ccgcccgcac
     1861 tacgacgtga tcgccgaatc caaggcgtac cgcaacggcg tgctgtcgat catcccgcag
     1921 aacgtcatca aggagttcga ggagcgcgcg ctcaaggccg gccacgccgg cgagacgaag
     1981 acgcttgacc agctgcgcag tggcgcgctg gccttctgcg ccaagaacgg catcgcggtc
     2041 gagcggcagt cgatcgcgaa cctgacctat gcagagctcc agggcctcag cgcatcggcc
     2101 gcgaacggcg tcgaggcctt ccgcgagtcc gccgaggcgc tcgggctgat ccgcaaagcc
     2161 gcagcgccgg cgcccgccct caccgagcag cagccggtca ccatgccgcc gctgcagacc
     2221 aagacggaga cgccggccta cgtcgatcgc gaaaccggcg aaattcagga gccgcagcgc
     2281 caacaggccg agcaatcgtc cggccccggc ttcgcggacg cgcatgccgc ggtgcgctcg
     2341 ggtgacttcg acctggcgcg cgacatcgcc cgtagcctcc cgacggccca gcgccagcag
     2401 atcgagactg cgatcgcgaa tctgtccgcc ccaccggccg aggagagcga gaaccccgcc
     2461 ccgacccagc aagcccccgc cgcccggcgc agccgcggcc aagccggtct cggcctcgag
     2521 taacccacca agcgcgcccg ccgccgagcg ggcgccggag cacgctcatg aaaatccact
     2581 ccatccacgt cgagggtttc ctcggcgcgc ggcaggtcga catcgccctc gtcgagccga
     2641 tcacgctgat tgcgggacag aacggcgccg gcaagagcag catcgccaac gcgatcgcgc
     2701 ttgcgctcaa cgccgagctc ggccgcgtcg atgtcaaaaa ggacgccgcc cgcctcgtca
     2761 ccgagggtgc caccgcggcc tgcgtgcaga tccagaccga cagcgccgac ttcgaggtgc
     2821 acatcaccaa ggccggcaag atcaccgatc acgccgccgg ccggaagccg caccccgccc
     2881 tgcccttcgc gctagacgcg tcgcggttct ccaggctgcc cgatgccgag cgccgcacgc
     2941 tgctgttcaa cctgctgggc atcgagacga cgcccgaggc gattgccgag cgtctcgctc
     3001 ggatgaaatg cgatgccgac aaggtcaagg ccatcggtcc tgccctgcgc gccggcttcg
     3061 tcgccggcga gaaacacgcc cgcgagcttg cgagtcaggc acgcggcaag tgggtggcgg
     3121 tcaccggaga gaaggcctac ggcgacaaga aggccgaagg atgggagccg ccgccggctg
     3181 cagcgctggc aggcgatccc gcggccctgg ccgagaacgc gagcgcccgc gccaacgagc
     3241 tcggcgagca gatcgccgcc ctgcagcaag agctcggcgc cgcgcgtgcc acgcgggagg
     3301 ccgctcagca gcgcgcagtc cgcatcgagc gcctgcgcga tcaggccggc atggtcgagc
     3361 gcatcaaggg ccggctggcc accgccgagg agaacgtggc ctcgtgcaag gcgcagttcg
     3421 ccggcgccgg cagcgacgat ccgaaggcgc cgggcagcta cctgctgcgg gggctggcca
     3481 gcgcgctcaa tgagttcgtc gcgctaacgg gcgaaagcca cggcgttgtc ggctaccact
     3541 tgaacggcga tgtcgccaga tggggcgagt tcgagtcgct gatcaaccgg tcggccgccc
     3601 atctcggcga gtaccgcaag ttgcacggcg accctgacgc cacgcccgcc gacccggtgc
     3661 gcctggccga gctgcgccac gcgctgcaga cggccgagtc cggtgctgcg aacgcccgcc
     3721 gggatctcgc cgcggccgaa gcggcaggcg cagaactcgc cgagctcgag aaagctgcgc
     3781 cgctcgaggc gccgaagatc gaggacatcg agacgcgcct ggccgatgcc cgccggcgcc
     3841 acgcagactg gcaggccgac gtccagaagt acctggaggc agccggcgcc gccgaacgtc
     3901 gtgcgcagca gatagccgac gccgcccggc accacgccga cgtgcagcag tggctggcaa
     3961 tcgccgacgc gctgtcgccc gatggcatcc aggctgcgat gctcgccgac gcgctggacc
     4021 cgatcaatgc ccgcctggcg gagaacgccg cgctcgccca gtggccggcg ccgaagatca
     4081 ccgcggacat ggcgatcgtg gtcgacggcc gcgcgatcgc gctgctgtcg gagtcggagc
     4141 gctggcgcac cgatgcgctg ctgacggtct cgctcgggca gctctccggc ctgcgcttcg
     4201 ccgtgctcga ccgcttcgac tgcctggaca tgaccggccg cgaggacctg ctgttctggc
     4261 tctctgacct ggccgaagcc cagcagatcg acacggtggt cgtgctcggc acgctcaagg
     4321 ccgcaccgcc cgccggcgcg ttgccgccgc acatcgaggc gcagtggatc gagcgcggcg
     4381 cgctgatccc cgccagtcag caaaccttgg aggcagcatg accaccgaca accaaactct
     4441 gcagcaactc ctcaccgagc gcgtgaccgc cttcgccaac ggcgaccgca tcgacgccaa
     4501 ccgcaagacg gtcgaggaca tgagcctgat gcccgacttc gcccaggtct
//
LOCUS       DAOQNN010000266         4522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11901_length_4522_cov_10.026640, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000266 DAOQNN010000000
VERSION     DAOQNN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11901_length_4522_cov_10.026640"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PKV97_12605"
     CDS             <1..740
                     /locus_tag="PKV97_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB06767.1"
                     /translation="ELRAANERLKELDRLKDDFMSSVTHELRTPLTSIRALSEMMLDD
                     PDIDVEDRQRFLGIIVSEAVRLSRLVNQVLDMAKIESGHAEWHNTDIDLCELVRHSGD
                     ATLQLFRDRGAELRLHLPESVPTLRADHDRLLQVMLNLLSNAAKFVPTGSGRVDVTLS
                     CDGERLRVAVKDNGPGIEPAQQPVVFEKFRQGGDERSRPQGTGLGLPISRQIVEHFGG
                     RLWLESVPGEGATFSFELPLKSAAGEG"
     gene            771..1139
                     /locus_tag="PKV97_12610"
     CDS             771..1139
                     /locus_tag="PKV97_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB06768.1"
                     /translation="MDKKVLIADDEQNIVISLEFLMKREGYQVEVANDGEEAVRRIRA
                     SRPDLVLLDVMMPKKSGFEVCQEIRSDPEMAGIRILMLTAKGRDTEVAKGLALGADAY
                     MTKPFSTRELVDKVRSLLEH"
     gene            1143..3452
                     /locus_tag="PKV97_12615"
     CDS             1143..3452
                     /locus_tag="PKV97_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNB06769.1"
                     /translation="MQARARFMLAVVVLFLLMTGPFLLTALIVWVETEGEARDLLLKV
                     LSPHLTLGTLLTAMGFAAGVGVVRVLFRQYVQGLLRMSENLRLMLGANRGFRVHPEGP
                     PEVQQLAVATNELASQRDVLLQDVEAQIGRAKASVELEKDRLAALMSELTQSVVVCNL
                     DGRVLLYNNRARAQFRALSDAPGVAGGGELIGLGRSIYGVFERNLIAHALDTIQQRLR
                     RGAAQAQANFVTTTRAGQLLRVQMAPVLSLAAPALVAAESTAVAAGSVTGVQQNAAAE
                     GDGAGNAGGGAFPAAPEVAAAAVTSERTLTGFILMLDNITRNFETESRRDQMLHNLTE
                     GNRASLANVRAAADMLEFEDLTDEMRTRFRKVVRDEVQAMSQRLDHTANEFADSLKAR
                     WPLEEMLGADLIAAAQRRIEARIGLPTKLEEVDESLWVKVDSFSLIQAITYLASRLSD
                     EFEVREVRFRLSAAGRLVHLDLIWSGQAMSTETVMSWELEPMKIEDESSPLTVRDVVD
                     RHDGEMWLEREKVRHRAFFRILLPAATPQEQAEAETYLRGESRPEYYDFDLFAWSEKS
                     HELDDRLLSELSYTVFDTETTGLNPSQGDEIIQVGATRILNGKLLRAESFEQLVDPER
                     PLAPESAKIHGITSEMLRGQPTIDKVLPAFHAFAADTVLIAHNAAFDMRFLQLKEEQT
                     GLRFDHPVIDTLLLSAVVHPNQESHRLEAIAERLGLTIVGRHTALGDAIVTAEVFLKL
                     IPLLAEKGIRTLRDAREAAEKTYYARVKY"
     gene            3490..>4522
                     /locus_tag="PKV97_12620"
     CDS             3490..>4522
                     /locus_tag="PKV97_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HNB06770.1"
                     /translation="MRRNPGFARQLRHYYGIYTLGFALFVVLLAIGESLGLSQQLIGH
                     VFLFVTIAIYATIGVLSRTSDVTEYYVAGRRVPALFNGMATAADWMSAASFIGLAGTL
                     YFSGFEGLAFVTGWTGGFVLVALLLAPYLRKFGQYTIPDFLGARYQGNVARLVGLAAA
                     VLASFVYLVAQIYGVGLVTSRFVSVEFEIGLFIGLAGILVCSFLGGMRAVTWTQVAQY
                     VILIIAYLVPVVILSYKLTGIPIPQAVYGTVLQQISAREDELLEAPTERAVRDLYSAR
                     ARDYADKIAALPASLEDERRRMIDELNRMRLDSAPARDIALTERALRDLPRTPAEARE
                     AWQRARAEAL"
BASE COUNT          711 a         1586 c         1522 g          703 t
ORIGIN      
        1 ccgagctgcg cgccgccaac gagcgcttga aggagctcga ccgcctcaag gacgacttca
       61 tgtcctcggt gacgcacgag ctgcgcacgc ccttgacctc gatccgcgcg ctctccgaga
      121 tgatgctcga cgaccccgac atcgacgtcg aggaccgcca gcgcttcctc ggcatcatcg
      181 tgtccgaggc ggtgcgcctg tcgcgcctgg tgaaccaggt gctcgacatg gccaagatcg
      241 agtccggcca tgccgagtgg cacaacaccg acatcgacct gtgcgaactg gtgcgccact
      301 cgggcgacgc caccctgcaa ctcttccgcg accgcggcgc cgaactgcgc ctgcacctgc
      361 cggagtcggt gccgaccctg cgtgccgacc acgaccgact gctgcaggtg atgctgaacc
      421 tgctgtcgaa cgcggcgaag ttcgtgccca cgggcagcgg tcgcgtggat gtgacgttga
      481 gctgcgatgg cgaacgcctg cgcgtcgcgg tgaaggacaa cgggccgggg atcgagccgg
      541 cgcagcagcc ggtggtgttc gagaagttcc gccagggcgg cgacgaacgc tcgcgaccgc
      601 agggtaccgg cctggggctg ccgatcagcc gccagatcgt cgagcacttc ggcggtcggc
      661 tgtggctgga gtcggtgccg ggcgagggcg cgacattctc cttcgagctg ccgctgaagt
      721 cggcggcggg cgagggatga catagaatat cgggcgggga ggagacgctg atggacaaga
      781 aagtactgat tgccgacgac gaacagaaca tcgtcatttc gctggaattc ctcatgaagc
      841 gcgagggcta ccaggtcgag gtggccaacg acggcgagga ggcggtgcgc aggatccgcg
      901 cctcgcgacc cgacctggtg ctgctcgacg tgatgatgcc gaagaagagc ggcttcgagg
      961 tctgccagga gatccgttcc gaccccgaga tggcgggaat ccgcatcctg atgctgaccg
     1021 ccaagggccg cgacaccgag gtcgccaagg gcctcgcgct cggcgccgac gcctacatga
     1081 ccaagccctt ctccacccgc gagctggtgg acaaggtgcg cagccttctg gagcactgaa
     1141 tcatgcaggc acgtgcgcga ttcatgctcg cggtcgtcgt gctgttcctg ctgatgaccg
     1201 ggcccttcct gctcacggcg ctgatcgtct gggtggagac cgagggcgag gcgcgcgacc
     1261 tgcttctgaa ggtgctgtcg ccgcacctca cgctcggtac cctgctcacc gcgatgggct
     1321 tcgctgccgg ggtgggcgtg gtgcgcgtgc tcttccgcca gtacgtccag ggcctgctgc
     1381 gcatgtcgga gaacctgcgc ctgatgctgg gtgccaaccg cggtttccgc gtgcaccccg
     1441 aggggccgcc cgaggtgcag cagctcgcgg tcgccaccaa cgagctcgcg agccagcgcg
     1501 acgtgctgct gcaggacgtg gaggcgcaga tcgggcgcgc caaggcttcg gtcgagctcg
     1561 agaaggaccg cctcgccgcg ctgatgtccg agctcaccca aagcgtggtg gtgtgcaacc
     1621 tcgacggccg cgtgctgctc tacaacaacc gtgcgcgcgc ccagttccgc gcgctctcgg
     1681 acgcgcccgg cgtggccggc ggcggcgagc tgatcgggct ggggcgctcc atctatggcg
     1741 tcttcgagcg caacctgatc gcgcacgcgc tcgacaccat ccagcagcgc ctgcgccgcg
     1801 gtgccgccca ggcacaggcc aacttcgtca ccaccacccg tgccggccag ctgctgcgcg
     1861 tgcagatggc gccggtgctg tcgctggccg cgccggcgct tgtggcggcg gagtcgacag
     1921 ccgtcgccgc agggagcgtg accggtgtgc agcagaatgc ggcagcggag ggcgacggcg
     1981 ctggcaatgc agggggcggc gcgttccccg cagcgcccga ggtcgccgcg gcagcggtca
     2041 cgtccgagcg cacgctcacc ggtttcatcc tgatgctcga caacatcacg cggaacttcg
     2101 agaccgagtc gcgccgcgac cagatgctgc acaacctcac cgagggcaac cgcgcctcgc
     2161 tcgccaacgt gcgcgcggcg gccgacatgc tcgagttcga ggacctcacc gacgagatgc
     2221 ggacgcgttt ccgcaaggtg gtgcgcgacg aggtgcaggc catgagccag cgccttgacc
     2281 acacggcgaa cgagttcgcc gactcgctta aggcgcgctg gccgctcgag gagatgctcg
     2341 gcgccgacct gatcgccgcg gcgcagcgcc gcatcgaggc ccgcatcggc ctgcccacca
     2401 agctcgagga ggtcgatgaa tccctgtggg tgaaggtcga cagcttctcg ctgatccagg
     2461 ccatcaccta cctcgccagc cggctgtcgg acgaattcga ggtgcgcgag gtgcgcttcc
     2521 gcctgagcgc ggccgggcgc ctcgtgcacc tcgacctgat ctggtcgggg caggcgatga
     2581 gcaccgagac ggtgatgagc tgggagctcg aaccgatgaa gatcgaggac gagagcagcc
     2641 cgctcaccgt gcgcgacgtg gtcgaccgcc acgacggcga gatgtggctc gagcgcgaga
     2701 aggtgcgcca ccgtgccttc ttccgcatcc tgctgccggc ggcgacgccg caggagcagg
     2761 ccgaggccga gacctacctg cgcggcgaga gccgtcccga gtactacgac ttcgatctct
     2821 tcgcgtggtc ggagaagtcg cacgaactcg acgaccgcct gctctccgag ctcagctaca
     2881 cggtgttcga caccgagacc accgggctca acccctcgca gggcgacgag atcatccagg
     2941 tcggcgccac ccgcatcctc aacggcaagc tgctgcgcgc ggaatccttc gagcagctgg
     3001 tggacccgga gcgtccgctc gcccccgagt cggccaagat ccatggcatc acctcggaga
     3061 tgctgcgcgg ccagcccacc atcgacaagg tgctgcccgc cttccacgcc ttcgccgccg
     3121 acaccgtgct gatcgcgcac aacgccgcct tcgacatgcg gttcctgcag ctcaaggaag
     3181 agcagaccgg cctgcgcttc gaccatccgg tgatcgacac cctgctgctg tcggcagtgg
     3241 tgcatcccaa ccaggagtcg caccgcctgg aggcgatcgc cgagcgcctg ggcctgacca
     3301 tcgtcggccg tcacaccgcg ctcggcgacg cgatcgtgac cgccgaggtc tttctcaagc
     3361 tgatcccgct gctcgccgag aagggcatcc gcaccctgcg cgacgcgcgc gaggccgccg
     3421 agaagaccta ctacgcgcgg gtcaagtact gatgaccggc cgccgcaggc cgcgcggaga
     3481 gcattcccct tgaggcgcaa ccccggcttc gcgcgtcagc tgcgccacta ctacggcatc
     3541 tacaccctcg gcttcgcgct tttcgtcgtg ctgctcgcga tcggcgagag cctgggcctg
     3601 tcgcagcagc tcatcggcca cgtcttcctg ttcgtgacga tcgcgatcta cgccaccatc
     3661 ggcgtgctct cgcgcacctc cgacgtcacc gagtactacg tcgccggacg ccgcgtgccg
     3721 gcgctgttca acggcatggc gaccgcggcc gactggatga gcgccgcgtc cttcatcggt
     3781 ctggccggta ccttgtactt cagcggcttc gagggcctgg ccttcgtgac cggatggacc
     3841 ggcggcttcg tgctggtggc cttgctgctc gcgccctacc tgcgcaagtt cggccaatac
     3901 accatccccg acttcctcgg cgcgcgctac cagggcaacg tcgcgcgcct ggtcggactg
     3961 gcggcggcgg tgctggcgag cttcgtctat ctggtcgcgc agatctacgg cgtgggcctg
     4021 gtgaccagcc gcttcgtcag cgtcgagttc gagatcgggc tcttcatcgg cctggccggc
     4081 atcctggtgt gctccttcct cggcggcatg cgcgccgtga cctggaccca ggtggcgcag
     4141 tacgtgatcc tgatcatcgc ctacctggtt ccggtcgtga tcctgtccta caagctcacc
     4201 gggattccga tcccgcaggc ggtttacggc accgtgctgc agcagatcag cgcgcgcgag
     4261 gacgagctcc tcgaggctcc caccgagcgc gcggtgcgcg atctctacag cgcgcgcgcg
     4321 cgcgactacg ccgacaagat cgccgcgctg cccgcctcgc tcgaggacga gcgccggcgc
     4381 atgatcgacg agctcaaccg catgcgcctg gacagcgcgc cggcgcgcga catcgcactg
     4441 accgagcgcg cgttgcgcga cctgccgcgc acgccggccg aggcccgcga ggcctggcag
     4501 cgcgcgcgcg ccgaggcgct cg
//
LOCUS       DAOQNN010000267         4506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_11958_length_4506_cov_8.837115, whole genome shotgun sequence.
ACCESSION   DAOQNN010000267 DAOQNN010000000
VERSION     DAOQNN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11958_length_4506_cov_8.837115"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..206)
                     /locus_tag="PKV97_12625"
     tRNA            complement(122..206)
                     /locus_tag="PKV97_12625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Leu,seq:cag)
     gene            246..3413
                     /gene="rnr"
                     /locus_tag="PKV97_12630"
     CDS             246..3413
                     /gene="rnr"
                     /locus_tag="PKV97_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNB06771.1"
                     /translation="MTRKTKKTDQQRSGAPSRRTQAAPQQGEIAPRANPPGRKAGRGT
                     LAAHAAPEPAAQGSTRHTGSPARGPGRSRTARKAAAVQASAVRLADPFYERETQQYDN
                     PLPSREYVLQILAERGVPMPLAELAGALDIAPHELDFFDRRLRAMERDGQVVRNRRDA
                     YLLPAKADLIKGRVEGHPDGFGFLRRDDGEPDVFLGPKEMREVLHGDRVMVRISGTDR
                     RGRPEGKLVEVLERANSRVVGRVINEHGVMIVVPENRRLAQDILVAPGGRKKPEPGQI
                     VTVELVEQPTKFAQPIGRIVEVLGNYADPGMEIEIALRKHDLPFEFSSEAKAQTRKLP
                     DVVRKKDWAGREDLTKLPLVTIDGETAKDFDDAVYCERQGKGYRLIVAIADVSHYVDA
                     ASALDKDAFDRGNSVYFPRRVIPMLPEKLSNGLCSLNPQLERLAMVADMNIAATGEIR
                     NYRFYPAVIWSHARLTYTKVAAALYDKDPAVRAELAALLPHLEQLDQLFRVLLKARAK
                     RGAIDFETTETRMIFDDNGKIAQIVPEVRNDAHRLIEECMLAANVCASDFLATREHPA
                     LYRVHDSPSEDKLAKLREFLKEFGLGLGGGDEPRAADFARLLEQVKDRPDAQLLQTVM
                     LRSLKQAMYSPDNVGHFGLAYESYTHFTSPIRRYPDLLIHRGIKAALAGEQYRPGDWE
                     QIGLHCSMTERRADDATRDVVAFLKCYFMQDRVGEEFVGSVSAVVPFGLFVALDDIFI
                     EGLLHISDLGSDYFHYDETRHALMGERTGKQFRLSDRVKVQLVRVDMATNKIDFRLIE
                     GPLPVEAKAPPKVAEVVAAEVVPVEGKKARKPRSKKAEVEERVEATPAPVVEAVPAAA
                     EPAAPKVRGKRTKKAAPEALAEVPVAPAVESTAAAAEPAAPKARGKRAKKAAPEVVAE
                     VLVAPVGESVTAAEASATPKARGKRAKKVVAGQVAEVVAAPAIEPVPAAAEPVAPKAR
                     GKRAKKAAIATVAEVVVAPVLEVTPDAAPPAAPKTRGKRAKKAVAGTATEVPAASAAE
                     ALESAPPVPARRAGRKTTTKGSDRG"
     gene            3406..4161
                     /gene="rlmB"
                     /locus_tag="PKV97_12635"
     CDS             3406..4161
                     /gene="rlmB"
                     /locus_tag="PKV97_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNB06772.1"
                     /translation="MAKAPENTPTRLIYGFHAVSAKLRHAPDSVLEIFLTADRKDARV
                     RKFLAQAESAGVRITQSEGDKLDALVGTRRHQGVVAKVDATRREIKLADVLDSLDENA
                     LILVLDGVQDPHNLGACLRVADAAGAHAVVAPKDRAVGLNATAVKVASGAADTVPYIT
                     VTNLARALREMQDAGVWVVGAAGEAEKTLYGVDQKVPVAWVLGAEGDGLRRLTRETCD
                     ELARIPMHGSVESLNVSVASGICLFEARRQRGS"
     gene            complement(4195..>4506)
                     /locus_tag="PKV97_12640"
     CDS             complement(4195..>4506)
                     /locus_tag="PKV97_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNB06773.1"
                     /translation="RSTARFAGWTFDANRHALLAPDGREVPLSAAEAGLLATLLRRPN
                     KILSREQLLGERDVDPFDRSIDVRISRLRRKLDDDPQNPRLIKTVYGAGYLFAVQVEW
                     V"
BASE COUNT          757 a         1657 c         1493 g          599 t
ORIGIN      
        1 acgcaagtcg cgacctgcgc atgcagcctg caccgcgcgt cctccagcgc gccgcatttg
       61 tccgcgtcaa cacggcgcaa aaacaaaaaa gccgctccga agagcggctt tcccgtgaaa
      121 ctggtgccca gaagaggact cgaacctcca cgcctcgcgg cgctagtacc tgaaactagt
      181 gcgtctacca attccgccat ctgggcaaga agacgcgcat tataagcacg caaagaagat
      241 tgtcaatgac tcgcaaaacg aaaaaaaccg atcagcagcg ctccggcgcc ccctcccgca
      301 gaacgcaggc cgcgccgcag cagggcgaga ttgcgccgcg cgccaacccg cccgggcgca
      361 aggccgggcg cggcacactt gccgcgcacg ccgcgcccga acccgctgcc caaggcagca
      421 ctcggcacac cggctccccg gcgcgcggcc ctggccgcag tcggaccgcg cgcaaggcgg
      481 cagccgtgca ggccagcgcc gtccgtctcg ccgacccctt ctacgagcgt gagacgcagc
      541 agtacgacaa cccgctcccc agccgcgaat acgtgctgca gatcctcgcc gagcgcggcg
      601 tgccgatgcc cctcgcggag ctcgccggcg cgctcgacat cgccccgcac gagctcgact
      661 tcttcgaccg tcgcctgcgc gcgatggagc gcgacggcca ggtcgtgcgc aaccgccgcg
      721 acgcctacct gctgccggcc aaggccgacc tcatcaaggg gcgcgtcgag ggccaccccg
      781 acggcttcgg cttcctgcgc cgcgacgacg gcgagcccga cgtctttctc ggtcccaagg
      841 agatgcgcga ggtgctgcac ggcgaccgcg tcatggtgcg catctccggc acggaccgcc
      901 gcggccgtcc cgagggcaag ctggtcgagg tgctcgagcg cgccaactcc cgcgtcgtcg
      961 gtcgtgtcat caacgagcac ggcgtgatga tcgtcgtgcc cgagaaccgc cgcctggcgc
     1021 aggacatcct ggtcgccccg ggcgggcgca agaagcccga gccaggccag atcgtcaccg
     1081 tcgagctggt cgagcagccc accaagttcg cccagccgat cgggcgcatc gtcgaggtgc
     1141 tgggcaacta cgccgacccc ggcatggaga tcgagatcgc gctgcgcaag cacgacctgc
     1201 ccttcgagtt ctcctccgag gccaaggcgc agacgcgcaa gctgcccgac gtggtgcgca
     1261 agaaggactg ggccggccgc gaggacctca ccaagttgcc gttggtcacc atcgacggcg
     1321 agaccgccaa ggacttcgac gacgcggtgt attgcgagcg ccagggcaag ggttaccgcc
     1381 tcatcgtcgc catcgccgac gtctcgcact acgtcgatgc cgccagcgcg ctcgacaagg
     1441 atgccttcga tcgcggcaac tcggtgtact tcccgcgccg cgtcatcccg atgctgcccg
     1501 agaagctctc caacggcctg tgctcgctca acccgcagct cgagcgcctg gccatggtcg
     1561 ccgacatgaa catcgcggcc accggcgaga tcaggaacta ccgcttctac cccgcggtga
     1621 tctggtcgca cgcgcgcctg acctacacga aggttgcggc ggccctgtac gacaaggacc
     1681 cggccgtgcg cgccgaactc gccgcgctgc tcccccacct cgagcagctc gaccagctct
     1741 tccgcgtgct gctcaaggcg cgcgcgaagc gcggcgcgat tgacttcgag accaccgaga
     1801 cgcgcatgat cttcgacgat aacggcaaga tcgcgcagat cgtccccgag gtgcgcaacg
     1861 atgcccaccg cctgatcgag gagtgcatgc tcgccgccaa cgtgtgcgcc tccgacttcc
     1921 tcgccacgcg cgagcacccg gcgttgtacc gcgtccacga ctcgccctcc gaggacaagc
     1981 tcgccaagct gcgcgagttc ctcaaggagt tcggcctcgg cctcggcggc ggcgacgagc
     2041 cgcgcgccgc cgacttcgcc aggctgctcg agcaggtcaa ggaccgcccc gacgcccaat
     2101 tgttgcagac cgtcatgctg cgttcgctca agcaggcgat gtacagcccg gacaacgtcg
     2161 gccacttcgg cctcgcctac gagtcctata cccacttcac ctcaccgatc cggcgctatc
     2221 ccgacctgct gatccaccgc ggcatcaagg ccgcgctcgc cggcgagcag taccgccccg
     2281 gcgactggga gcagatcggc ctgcactgct cgatgaccga gcgccgcgcc gacgacgcca
     2341 cccgcgacgt ggtcgccttc ctcaagtgct acttcatgca ggaccgcgtc ggcgaggagt
     2401 tcgttggcag cgtgtcggcg gtggtgcctt tcggtctctt cgtggcgctc gacgacatct
     2461 tcatcgaggg gctgctgcac atctccgacc tcggcagcga ttacttccac tacgacgaga
     2521 cccgccatgc gctcatgggt gagcgcaccg gcaagcagtt ccgcctgtcc gaccgggtca
     2581 aggtgcagct cgtgcgggtg gatatggcca ccaacaagat cgacttccgc ctcatcgagg
     2641 ggccgctccc ggtcgaggcc aaagccccgc cgaaggtcgc cgaagtggtg gccgcggagg
     2701 tcgtgcccgt ggaagggaag aaggcgcgca agccgcgcag caagaaggct gaggtggagg
     2761 agagggtcga ggccacacct gcgccggtgg tcgaggctgt gcctgccgca gcggagcccg
     2821 ccgcgcccaa ggtgcgcggc aagcggacga agaaggccgc acccgaagcg cttgcggagg
     2881 tgcccgtcgc gccggcggtc gagtccacgg ctgccgcagc ggagcccgcc gcgcccaagg
     2941 cgcgcggcaa gcgggcgaag aaggctgcgc ccgaagtggt cgcggaggtg ctcgtcgcac
     3001 cggtgggcga gtctgtgact gctgccgagg cgtcggcgac gcccaaggcg cgcggcaagc
     3061 gcgcgaagaa ggtggtcgcc gggcaggtgg ccgaggtcgt ggctgcgccg gcgatcgagc
     3121 ctgtgcccgc cgccgcggaa cctgtcgcgc ccaaggcgcg cggcaagcgt gcgaagaagg
     3181 ctgcgatcgc aacggtcgcc gaggtcgtcg tcgcaccggt gctcgaggtt acgccggatg
     3241 cggctccgcc cgccgcgccc aagacacgcg gcaagcgcgc gaagaaggcc gtcgccggca
     3301 ccgcgaccga agtccccgcc gcgtccgccg ccgaggcgct agaatccgcg cccccggtac
     3361 cggcccgacg tgccggccgc aagaccacca caaagggctc cgaccgtggc taaagcaccc
     3421 gaaaacacac ccacccggct gatctacggc ttccacgccg tctccgccaa gctgcgccac
     3481 gcgcccgact cggtgctcga gatcttcctc accgccgatc gcaaggacgc gcgcgtgcgc
     3541 aagttcctcg cccaggccga gagtgccggc gtacgcatca cccagagcga gggcgacaag
     3601 ctcgacgccc tcgtcgggac ccgccgccac cagggggtgg tcgccaaggt cgacgccacc
     3661 cggcgcgaga tcaagctcgc cgacgtgctc gacagcctcg acgagaacgc gctcatcctc
     3721 gtcctcgacg gcgtgcagga tccgcacaac ctcggcgcct gcctgcgcgt ggccgatgct
     3781 gccggcgccc acgccgtggt cgcgcccaag gatcgcgcgg tgggtctcaa cgccaccgcc
     3841 gtcaaggtcg ccagcggcgc cgccgacacc gtgccctaca ttaccgtcac caatctcgcg
     3901 cgcgcgctgc gcgagatgca ggacgccggc gtgtgggtcg tcggcgccgc cggcgaggcc
     3961 gagaagaccc tctacggggt cgaccagaag gtccccgtcg cctgggtgct cggcgccgag
     4021 ggcgacggcc tgcgccgcct cacccgcgag acctgcgacg agctcgcgcg catcccgatg
     4081 cacggcagcg tcgagagcct caacgtgtcc gtcgccagcg gcatctgcct gttcgaggcc
     4141 aggcgccagc gcggctcctg aagctgtggt ggtcccggcc tgccccttgg cctctcacac
     4201 ccactccacc tgcaccgcaa acagataccc cgcgccatac accgtcttga tcaggcgggg
     4261 gttctgcggg tcgtcgtcga gcttgcggcg caggcgcgag atgcggacgt cgatgctgcg
     4321 gtcgaaggga tcgacgtcgc gttcgccgag gagctgttcg cgcgacagga tcttgttggg
     4381 gcgacgcagc agggtggcga ggaggccggc ctcggctgcg gaaagcggga cctcgcggcc
     4441 gtcgggggcg aggagggcgt ggcggttggc gtcgaaggtc cagccggcga agcgcgccgt
     4501 gctgcg
//
LOCUS       DAOQNN010000268         4474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_12124_length_4474_cov_8.524327, whole genome shotgun sequence.
ACCESSION   DAOQNN010000268 DAOQNN010000000
VERSION     DAOQNN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12124_length_4474_cov_8.524327"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..1342)
                     /locus_tag="PKV97_12645"
     CDS             complement(323..1342)
                     /locus_tag="PKV97_12645"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNB06774.1"
                     /translation="MNATPAPYPRLVADIGGTNARFALVEAPGAAPTHLRALRCAEHG
                     GPEAALRAWLADTGARLPAYAAFGIATPIDGDGVAMTNHPWRFSIGALCGALGLRRLT
                     VVNDFTALALALPALGDGDLVRVGGGEPHAGAARALIGAGTGLGVSGLLPVPGGWVPL
                     QGEGGHVTLPASCTREAAVVAWLAARHGHVSAERVLSGPGLVVLHDTLRALDGEARVE
                     RTPAEISERALAGGCRHCVEALELFCALLGTVAGDVALTLGARGGLYIGGGIVPRLGD
                     FFLRSAFRERFVAKGRFRPWLERIPIWVVVAPHAALTGASAALDSAIELGFTALAEPR
                     GHVSP"
     gene            1728..3446
                     /locus_tag="PKV97_12650"
     CDS             1728..3446
                     /locus_tag="PKV97_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNB06775.1"
                     /translation="MPPSAAPSVGPQPASSPAAPWWKSAVAYQLYPRSFMDSDGDGIG
                     DLRGILARLDYLQWLGVDVIWICPIFDSPNDDNGYDIRDYRKILSTFGTMEDFDRLLA
                     EVHRRGMRLILDLVLNHTSDEHPWFLESRASPDNPKRDWYIWREGRDGHPPNNWESIF
                     RGPAWTRDPATGQYYLHLFTRRQPDLDWTNPEMRAAFHDIVRWWLDKGIDGFRLDAVS
                     HIRKMPGLPDLPNPRGLPWVPSFAMHMNVDGVLDTIGELCRETFRRYDVMTVGEANGV
                     GPREAVEWVGSDRGRLDMIFQFEHLALWSRAPGAPLDVVALKKVLSRWQHALHGKGWN
                     ALFLENHDIPRVVSKWGDTGALWRESATALATMYFLMEGTPFIYQGQEIGMTNGVFER
                     IEDFDDVLAKNDYAQRRLAGEEEGAIVADLVLTGRDPARTPMQWDDGPQAGFTRGRPW
                     LAVNPNHRRINVAQQRHDPASVLNHYRRLIALRKAEPALVLGDYRLLMKDDAQIYAYQ
                     RRLDGERIAVIVNLSPHPARFDHPGVVFRHERLLLANRAVEAHPAAHALELAPYEARV
                     YRVGKM"
     gene            complement(3653..>4474)
                     /locus_tag="PKV97_12655"
     CDS             complement(3653..>4474)
                     /locus_tag="PKV97_12655"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HNB06776.1"
                     /translation="PADPLARIARWLREADGLIVAAGAGMGVDSGLPDFRGPEGFWRA
                     YPALGRAHVRFEEIANPAAFERDPALAWGFYGHRLALYRRTVPHRGFALLQEIAAGLR
                     GGAFVFTSNVDGQFQKAGFADARIMEVHGSIHHLQCQRGCRGAVWCAEGFEPQVDEEA
                     CRLLGEPPRCPHCGGVARPNILMFGDWAWIPRRTEAQEAGLVAWLEHVRRPVVIELGA
                     GENVPTVRRFAESIGGRLVRINPQAEPTLPAGAIHLRCGALEGIATVHAALVGHR"
BASE COUNT          692 a         1679 c         1533 g          570 t
ORIGIN      
        1 accgccggtg agcgcgacgg cgccccgatc gcgactcgcg tcgctcccac agccccaccc
       61 ggacgccggc aacagagtgc acgcgctggt ttttgtggga gcgacgcaag tcgcgaccgc
      121 acgattgcgc atgcggcgac cgccggtgag cgcgacggcg ccccgatcgc gactcgcgtc
      181 gctcccacag ccccacccgg acgccggcaa cagagtgcac gcgctggttt ttgtgggagc
      241 gacgcaagtc gcgatcgcac gattgcgcat gcggcgaccg ccggtgaacg cggcggcgcc
      301 ccgatcgcga ctcgcgtcgc gcctacgggg aaacgtgccc gcgcggctcg gcgagcgcgg
      361 tgaagccgag ctcgatggcg ctgtcgagtg ccgccgaggc gccggtgagg gcggcgtggg
      421 gggcgacgac cacccagatc gggatgcgtt cgagccaggg gcgaaagcgg cccttggcga
      481 cgaaacgttc gcggaacgcg gagcgcagga agaaatcccc cagccgcggc acgatgccac
      541 cgccgatata cagcccgccg cgcgcgccca gggtgagcgc gacgtcgccc gccaccgtgc
      601 cgagcagcgc gcagaagagc tcgagtgcct cgacgcagtg gcggcagccg ccggccagcg
      661 ccctttcgct gatctccgcc ggcgtgcgct cgacacgcgc ttcgccgtcg agcgcgcgca
      721 aggtgtcgtg gagcacgacc agaccgggac ccgagagcac gcgctcggcc gagacatggc
      781 catggcgggc ggcgagccag gcgaccaccg cggcctcgcg tgtgcaagag gccggcagcg
      841 tcacgtgccc gccctcgcct tgcagcggca cccagccgcc cggcacgggc agcaggcccg
      901 aaacgccgag cccggtgccg gcgccgatca acgcccgcgc ggcgcccgca tgcggctcgc
      961 cgccgccgac gcggaccagg tcgccgtcgc cgagcgcggg cagcgcgagc gcgagcgcgg
     1021 tgaaatcgtt caccacggtc agccggcgca ggccgagcgc gccgcacagc gcgccgatcg
     1081 aaaagcgcca gggatgattg gtcatcgcca cgccgtcgcc gtcgatcggc gtggcgatgc
     1141 cgaaggcggc gtaagcgggc aggcgggcgc cggtgtccgc caaccaggcg cgcaacgcgg
     1201 cctccgggcc gccgtgttcc gcgcagcgca gcgctcgcag atgcgtcggt gccgctccgg
     1261 gcgcctcgac gagggcgaag cgcgcgttgg tgccgccgat gtcggcgacc aggcggggat
     1321 agggagcagg ggtggcgttc atgtcccccg attctgccac gtgcaaaggc gcttgccggc
     1381 ccgggtgtcc gtagctgccc caaggcacct gcctgtaatc gccgtccccc gattccgttc
     1441 ccccgcgccg tggtggggcc ggatcaccct gatcaggacc cccgtcgcgc ccacgggggc
     1501 tacccgccgc cagccggcct tgtccgcagg agcgacgcaa gtcgcgattc tccgcctgcg
     1561 tcgcccggtg ctccagccgg gggggcgggg gcgcagcaat tcaaggttcc gtcgcgcctg
     1621 cgatggcaac aggcgcaagg gttcgtccgg cctgcgttcg tcatcccgcg ggcgcgggcc
     1681 tacaatcgaa cgcggcctcc cgaacaaccc gcctgctcgg tgcaatcatg cccccttccg
     1741 cagccccctc cgtcggcccg cagccggctt cgagccccgc tgccccctgg tggaaatccg
     1801 ccgtcgccta ccagctctac ccgcgcagct tcatggacag cgacggtgac ggcatcggcg
     1861 acctgcgcgg catcctcgcg cggctcgact acctgcaatg gctgggcgtc gacgtgatct
     1921 ggatttgccc gatcttcgac tccccgaacg acgacaacgg ctacgatatc cgcgactacc
     1981 gcaagatcct gtcgaccttc ggcaccatgg aggatttcga ccgcctgctc gccgaagtcc
     2041 accgccgcgg catgaggctg atcctcgacc tggtgctcaa ccacaccagc gacgagcacc
     2101 cctggttcct cgagtcgcgc gcctcgcccg acaaccccaa gcgcgactgg tacatctggc
     2161 gcgagggccg cgacgggcat ccgcccaaca actgggagag catcttccgc ggccccgcgt
     2221 ggacccggga cccggccacc gggcagtact acctgcacct gttcacccgc cgccagcccg
     2281 acctcgactg gaccaatccg gagatgcgcg cggccttcca cgacatcgtg cgctggtggc
     2341 tggacaaggg catcgacggc ttccgcctcg atgcggtgag ccacatccgc aagatgccgg
     2401 gcctgcccga cctgcccaat ccgcgcggcc tgccctgggt gccgtccttc gccatgcaca
     2461 tgaacgtcga cggcgtgctc gacaccatcg gcgagctctg ccgcgagacc ttccgccgct
     2521 acgacgtgat gaccgtgggc gaggccaacg gcgtcggccc gcgcgaggcg gtggagtggg
     2581 tgggcagcga ccgtggccgg ctcgacatga tcttccagtt cgagcacctg gcgctgtggt
     2641 cgcgcgcgcc cggcgcgccg ctcgacgtgg tcgcgctcaa gaaggtgctg tcgcgctggc
     2701 agcatgcgct gcacggcaag ggctggaacg cgctcttcct ggagaaccac gacatccccc
     2761 gcgtcgtctc caagtggggc gacaccggcg cgctgtggcg cgagagcgcg accgcgctgg
     2821 cgacgatgta cttcctgatg gagggcacgc ccttcatcta ccagggccag gagatcggca
     2881 tgaccaacgg cgtcttcgag cgcatcgagg acttcgacga cgtgctggcg aagaacgact
     2941 acgcgcagcg ccgcctggcc ggcgaggagg agggcgcgat cgtcgccgac cttgtcctca
     3001 ccggccgcga cccggcgcgc acgccgatgc agtgggacga cgggccgcag gcgggcttca
     3061 cgcgcggacg gccgtggctg gcggtcaatc ccaaccatcg ccgcatcaac gtcgcccagc
     3121 agcgccacga cccggcttcg gtgctcaacc actaccgccg cctgatcgcg ctgcgcaagg
     3181 ccgagcccgc gctggtgctc ggcgactatc gcctgctgat gaaggacgac gcgcagatct
     3241 acgcctacca gcgccggctg gacggcgagc gcattgcggt gatcgtcaac ctgagccctc
     3301 acccggcgcg cttcgatcat cccggcgtgg tgttccgcca cgaacgcctg ctgctcgcca
     3361 accgcgccgt cgaagcgcac cctgccgccc acgcgctcga gctcgcaccc tacgaggccc
     3421 gcgtataccg cgtgggaaaa atgtaggcgc gccgcaaggc gcgaaacggg cgctccggag
     3481 atcgacgcgc gtcgcagtct ccagcgccgc attcgcgcct tgcgacgcgc ctacagggac
     3541 gcgcggagcc gctacgacgt ccatcgccgc acgtcgacag ctgatcgccg gttacgcccg
     3601 cagccgtgcc ggttcatgta ggagcgccgc aaggcgcgaa tcgggcgtcg ggtcagcggt
     3661 gcccgaccag ggccgcatgg acggtggcaa tgccttcgag cgcaccgcag cgcaggtgga
     3721 tcgcgcccgc cgggagcgtc ggctccgcct gcgggttgat gcgcaccagc cgcccgccga
     3781 tcgactcggc gaagcggcgc accgtgggca cgttctcgcc cgcgccgagc tcgatcacca
     3841 ccggcctgcg cacgtgctcc agccaggcga ccagccccgc ctcctgcgcc tcggtgcggc
     3901 gtggaatcca ggcccagtcg ccgaacatca ggatgttggg gcgcgcgacg ccgccgcaat
     3961 gcgggcagcg cggcggctcg ccgagcagtc tgcaggcttc ctcgtccacc tgcggctcga
     4021 agccctccgc gcaccacacc gcgccccggc agccgcgctg gcactgcagg tgatgaatcg
     4081 agccgtgcac ctccatgatc cgcgcatcgg caaagccggc cttctgaaac tgcccatcca
     4141 cattcgaggt aaagacgaag gcgccgccgc gcaatcccgc ggcgatctcc tgcagcagcg
     4201 cgaagccccg gtgcggcacg gtgcgccggt agagcgccag gcggtgcccg tagaaccccc
     4261 aggcgagcgc cggatcgcgc tcgaacgcgg ccggattggc gatctcctcg aagcgcacgt
     4321 gggcgcggcc gagcgccgga taggcccgcc agaagccctc ggggccgcgg aaatccggca
     4381 gcccggaatc cacccccatg cccgcgcccg cggcgacgat cagcccgtcg gcctcgcgca
     4441 gccatcgggc gatgcgggca aggggatcgg cagg
//
LOCUS       DAOQNN010000269         4449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_12224_length_4449_cov_9.706190, whole genome shotgun sequence.
ACCESSION   DAOQNN010000269 DAOQNN010000000
VERSION     DAOQNN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12224_length_4449_cov_9.706190"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..539
                     /locus_tag="PKV97_12660"
     CDS             261..539
                     /locus_tag="PKV97_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HNB06777.1"
                     /translation="MTAAQPKNPLHGLTLERIVTDLVACFGWAGLGRRIDIRCFNHEP
                     SIVSSLKFLRRTPWAREQVEALYLDTLRAAARPGAATDPDTPRSTPNT"
     gene            536..1393
                     /locus_tag="PKV97_12665"
     CDS             536..1393
                     /locus_tag="PKV97_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HNB06778.1"
                     /translation="MKTPSRIEPLVADGLVDHVLRQLMSGKEAMVYVVQCGDELRCAK
                     VYKEANKRGFHKAADYTEGRKVKNSRQARAMAKGSRYGRQEQEAAWQHAEVDALRRLA
                     AAGVRVPVPYDFHEGVLLMELVADEDGEPAPRLNDVVLDEADARRFHAELVRQVVRML
                     CAGIVHGDLSEYNVLVDRDGPVIIDLPQAVDAAANNNARAMLERDVGNLAAYFGQFAP
                     DLLDTAYGKEIWALYESGKLQPESALTGVFEVEAGHADVDEVMQVIEAARREEAARQA
                     GRAEAAGQR"
     gene            complement(1629..2534)
                     /locus_tag="PKV97_12670"
     CDS             complement(1629..2534)
                     /locus_tag="PKV97_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNB06779.1"
                     /translation="MNPLVRIQPRSADLGDGMRVRRTLPNRQQRMVGAWCFLDHAGPV
                     HFEPGKGLHVGAHPHIGLQTFTWLIEGEVLHRDSLGNEQLIRPGQVNLMTAGHGIAHT
                     EDSLHDGVRLHAAQLWIALPPEHQDCAPAFDHYPTLPQWREGGAELTLLAGRYGQRTA
                     PARIHSALVGIDIACDEASALTLALDPGFEYGLMALVGDLKIDGETIRTDELAYLGKG
                     RGELHLALPAGARVLFLGGEPFDEPVQMWWNFVGFSRADIAQAQAAWERGDERFGAVG
                     DGRARRLVAPPLPWRGPGDTRLPHT"
     gene            2749..3084
                     /locus_tag="PKV97_12675"
     CDS             2749..3084
                     /locus_tag="PKV97_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06780.1"
                     /translation="MNWIMAVSIGFCVLLSSGTAMAAGAGQEGGMASVQAPAQQQEKK
                     EGAKTAVTEADPGNRASLLYIVLIGGVMVVAMKMAIAYGKKKLDEEREAAREKAAREE
                     RQNKSQAQG"
     gene            3329..4243
                     /locus_tag="PKV97_12680"
     CDS             3329..4243
                     /locus_tag="PKV97_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNB06781.1"
                     /translation="MVRALYSRAGSHAGVGLLLLVLFFLYAERAFGLLPADHAVQAWL
                     DPVRNAALALALFTGAHLLHALASARLSQRRKGRKPVPKVLLDLLRVFLFALAALGGL
                     SLLFRHDLSGILTGSGLVLAVLGFAIRNVVADTLSGIALGIEAPFRIGDWVRIENLAQ
                     GRVQEIGWRTTRLVTGDSTYVILPNSQISRQRITNFSAPKKEYRDHAELTLPVALPVA
                     EARELMRAALTHAASIAEDKPPEVQLVSYGPGGITYRVKYWVPQHDREPVCRDEVLGL
                     VDAALRARGVVLAQPLAVSACQHAEAAS"
BASE COUNT          682 a         1402 c         1639 g          726 t
ORIGIN      
        1 aggcgctgcg gaaggccttg ttcaagtaca agctgcacgc ggacgaggag ctgttcgaga
       61 aggcgttcag ttatatccgg cagtattact gagcgacccg agcatcaacg ggcaaaacga
      121 cacatgacca cgcagaccca tttcagcctt ctgccgcccg ttacaccccg gattgacaac
      181 gcgcgccagc gcggagtatc agtcactccc cctgcagcaa gcgatccgca caaccgtcag
      241 tctcgacgcg agaagacccg atgaccgcag cacaacccaa gaatccgctc catggcctga
      301 cgctcgaacg gatcgtgacc gacctggtcg cctgcttcgg ctgggcgggc ctggggcggc
      361 ggatcgacat ccgctgcttc aaccacgaac cgagcatcgt ctcgagcctc aagttcctgc
      421 gtcgcacgcc gtgggcgcgc gagcaggtgg aggcgctgta tctcgatacc ctgcgtgcgg
      481 ccgcccggcc gggcgcggca acggaccccg acaccccgcg gagcaccccg aacacgtgaa
      541 gaccccgagc agaattgaac cgctggtggc cgacggcctc gtcgaccacg tcctgcgcca
      601 gctcatgagc ggcaaggagg ccatggtcta tgtcgtgcag tgcggcgacg agctgcgctg
      661 cgccaaggtg tacaaggagg ccaacaagcg cggcttccac aaggcggccg actacaccga
      721 ggggcgcaag gtcaagaaca gccgtcaggc gcgcgcgatg gccaagggct cgcgctatgg
      781 ccgccaggag caggaggcgg cgtggcagca cgccgaggtc gacgcgctgc ggcggctcgc
      841 cgcggccggg gtgcgcgtgc ccgtgcccta cgacttccac gagggcgtgc tgctgatgga
      901 gctggtggcc gacgaggacg gcgagccggc gccgcggctc aacgatgtgg tgctcgacga
      961 ggcggatgcg cggcgcttcc atgccgagct ggtgcgccag gtcgtgcgca tgctgtgcgc
     1021 cggcatcgtc catggggatc tgtccgaata caacgtgctg gtcgatcgcg acgggccggt
     1081 gatcatcgat ctgccgcagg cggtggatgc ggcggccaac aacaacgccc gcgccatgct
     1141 cgagcgcgat gtcggcaacc tcgccgccta cttcgggcag ttcgcccccg acctgctcga
     1201 caccgcgtat ggcaaggaga tctgggcctt gtacgagtcg ggcaagctgc agcccgagag
     1261 cgcgctcacc ggggtcttcg aggtggaggc cgggcacgcc gatgtcgatg aggtgatgca
     1321 ggtgatcgag gcggcgcgtc gtgaggaggc cgcgcgccag gcgggccggg cagaggccgc
     1381 cggccagcgc tgagcggcga ggcggactcc gatgcgcgct tgcccgggcg tctcgcccga
     1441 cggggtcgag tgcgatcggc cgatccgatg ttccattggt cggtccggat cgtcggtgac
     1501 gtgcgtcgca gactcgacag cattcgtacg cgcgcggacc gcgcgcaggc tgcccggctc
     1561 gagggctggc tgggggcggt gcttgccgac cgctcggacc ggagtccggg tttcgacctc
     1621 gactgcgctc aggtgtgggg gaggcgcgtg tcgcccggcc cccgccatgg cagaggcggc
     1681 gccacgaggc gtcgcgcccg gccgtcaccg accgcgccga agcgctcgtc gccgcgctcc
     1741 cacgcggcct gcgcctgcgc gatgtcggct ctgctgaagc cgacgaagtt ccaccacatc
     1801 tgcaccggtt cgtcgaacgg ctcgccaccc aggaacagca cccgcgcgcc tgcgggcagg
     1861 gccaggtgca gctcgccgcg tcctttgccg aggtaggcga gctcgtctgt acggatcgtc
     1921 tcgccgtcga tcttcagatc gcccacgagc gccatcagcc cgtactcgaa accggggtcg
     1981 agcgcgagcg tgagcgcgct cgcctcgtcg caggcgatgt cgatgccgac cagcgcggag
     2041 tggatgcgcg ccggcgcggt ccgttggccg tagcggccgg cgagcagggt gagttcggcg
     2101 ccgccttcgc gccattgcgg cagcgtgggg tagtggtcga aggccggggc gcaatcctgg
     2161 tgctccggcg gcagggcgat ccacagttgc gcggcgtgca gccgcacgcc gtcgtgcagc
     2221 gaatcctcgg tatgggcgat gccgtggcct gcggtcatga ggttgacctg tcccgggcgg
     2281 atgagctgct cgttgcccag gctgtcgcgg tgcagcacct cgccctcgat cagccaggtg
     2341 aaggtctgca ggccgatatg cggatgcgcg ccgacgtgca ggcccttgcc gggctcgaag
     2401 tgcaccggcc cggcgtggtc gaggaagcac cacgccccaa ccatgcgctg ctggcgattg
     2461 gggagggtgc ggcggacgcg catgccgtcg ccgaggtcgg cgctgcgggg ctggatgcgg
     2521 acgagcgggt tcatggtctg tgtcctcgtg gggctgctgc cttgggcgtc gtggggattc
     2581 cccgtggaga gattcttgcg gcgcgtcgac cgcggtgggc tgttcgggtg ccgagtcccg
     2641 gcggggttct tcagtacaat gaccagaggc tcgacagatc gatgtgtcga ggcgccgctc
     2701 gctggaaaag tggacagcgc gtgacgagat cgacgaatga ggatttcgat gaattggatc
     2761 atggcagtgt cgatcggttt ctgtgtcctc ctttcgagtg gcacggccat ggctgcgggc
     2821 gccggacagg aaggggggat ggcgagcgtg caggcgcccg cgcagcagca ggagaagaag
     2881 gaaggcgcga agacggcagt gacggaggcg gaccccggca atcgagcttc gttgctgtat
     2941 atcgtgctga tcggtggggt gatggtcgtg gccatgaaga tggcgatcgc ttacggcaag
     3001 aagaagctgg acgaggagcg cgaggccgcc cgagagaaag ctgcgcgtga ggagcgacag
     3061 aacaagtcgc aagcgcaggg gtgaggcagg cggggccctg cgtttgaaaa cgcggaatcg
     3121 atccccatat agaaacgatt ggccatcctg gccacccacc cccaggaact tccggagacc
     3181 atgtgatggg ctgctgcggc cgcgcgagat tcgccttgtg catcgaccag gcacatgcct
     3241 ggccccggcc tcgatagcgc cttgccggcg cggatcggca ggccggggtc ggtggagtgt
     3301 cttttcctct cgtctcgaag gtcctgccat ggttcgcgcg ctgtactccc gcgccggctc
     3361 gcatgccggc gtcggtctcc tcctcctggt gttgttcttc ctgtatgccg agcgggcgtt
     3421 cgggttgctg ccggccgatc atgccgtgca ggcctggctc gatccggtgc gcaatgcggc
     3481 gctcgcgctc gccctgttca ccggcgcgca tctgctgcac gcgctcgcgt cggcgcggct
     3541 gtcgcagcgg cgcaaggggc gcaagccggt gcccaaggtg ctgctggacc tgctgcgggt
     3601 gttcctgttt gcgctggcgg cgctgggcgg gctgtcgctg ctgttccgcc acgacctgtc
     3661 ggggatcctg accggctcgg gcctggtgct ggcggtgctg ggcttcgcga tccgcaacgt
     3721 ggtggccgac acgctgtcgg ggatcgcgct cgggatcgag gcgccgttcc gcatcggcga
     3781 ctgggtgcgc atcgaaaacc tggcccaggg gcgggtgcag gagatcggct ggcgcacgac
     3841 gcggctggtg accggggaca gcacctacgt gatcctgccc aacagccaga tctcgcggca
     3901 gcggatcacc aatttcagcg cgccgaagaa ggaatatcgc gaccatgccg agctcaccct
     3961 gccggtcgcg ctgccggtgg ccgaggcgcg ggagctgatg cgcgcggcgc tgacgcatgc
     4021 ggcgagcatc gccgaggaca agccgcccga ggtgcagctg gtgtcctatg gccctggggg
     4081 catcacctat cgggtgaagt actgggtgcc gcagcacgac cgcgagccgg tctgccgcga
     4141 cgaggtgctc gggctcgtcg atgcggcgct gcgtgcgcgc ggggtcgtgc tggcgcagcc
     4201 gctggcggtg tccgcgtgcc aacatgcgga agccgcgtcc taggaacagg cttgcctgcg
     4261 cggctcgtcg gggcggggtg tggcgctgtc ggtctttttt acactcggag acgggtgtcg
     4321 acggaagtcg tcgataaatc agcggcttac ggtttggttc ggttcttgct tgagtctttt
     4381 cggggagtgt tggataccgg ggaggagcga atcatgaggc aggcttgcgc atcgatcctt
     4441 gcaactgcg
//
LOCUS       DAOQNN010000270         4389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_12508_length_4389_cov_9.996078, whole genome shotgun sequence.
ACCESSION   DAOQNN010000270 DAOQNN010000000
VERSION     DAOQNN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12508_length_4389_cov_9.996078"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKV97_12685"
     CDS             <1..237
                     /locus_tag="PKV97_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB06782.1"
                     /translation="AIELAPAVRVNGVSPGPIEWPEDDQFPPAERERIVAHTLLGRVG
                     SPDDIARTVAFLMFDAPYITGQILAVDGGRSAHL"
     gene            304..1257
                     /gene="ttcA"
                     /locus_tag="PKV97_12690"
     CDS             304..1257
                     /gene="ttcA"
                     /locus_tag="PKV97_12690"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004384785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNB06783.1"
                     /translation="MTAASAPAAALPTTEAGGDARFSNTFLRLKKKLERGVGEAIGDY
                     NMIGDGDTVMVCVSGGKDSYTLLSCLLALRERAPVDFRIVAMNLDQKQPGFPDHVLPA
                     YFESIGVEYRIVTEDTYSIVKDKIPEGKTTCSLCSRLRRGIIYRTATEIGATRIALGH
                     HRDDMLETLFLNMFFGGKIKAMPPKLVSDDGKHVVIRPLAYCTEADIARFARTMDFPI
                     IPCNLCGSQENAQRKLIKNMLQGWARDYPGRIESLATSLKNVVPSHLSDARLFDFVGL
                     TQHTVVEEGDTAFDPPELPDRTLGASVKLFRAGDQEERECA"
     gene            1383..2255
                     /locus_tag="PKV97_12695"
     CDS             1383..2255
                     /locus_tag="PKV97_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNB06784.1"
                     /translation="MPERKNLCVLVALHPVDAVLAERLGADEARHAAERHARRIERAI
                     VMQGGQVLLGGAEQTIAGFERSDAAVHAASEALDRIHGLPPLLGSKQTVRIGVHYGVL
                     DNDGEEPAGEGPAVARQLAHLAESGQALISGTAVMVLGGAARQQVGSQPLSGPVWTRV
                     GIPVHAIGQRSGLVTSVPGAARLSSRLRLRHQQEVLFVEELRPVLLLGRELANDVTIM
                     DPRASRQHARIERRPEGFVLFDQSTNGSFVAIEGKPECFVRQEQMLLDGPGRLGCGFS
                     TTELERDLVFFDIV"
     gene            complement(2335..2889)
                     /locus_tag="PKV97_12700"
     CDS             complement(2335..2889)
                     /locus_tag="PKV97_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HNB06785.1"
                     /translation="MYVICKIVLRILRRTGRGDKIMKRILGATGVVLVLAALTPAQAW
                     ANPQHERMKRCNAEAKTQALGGDARKAFMSTCLSGKHEAAAASAPGAAPALASGSAAG
                     VPAAAPVAAPAMVPAAASAVAPAAASAAGAAAPALAVAPTAGSAQKVSSEDMARQKAC
                     NRAATEQSLRGAKRKAFIGECMSG"
     gene            2953..3366
                     /locus_tag="PKV97_12705"
     CDS             2953..3366
                     /locus_tag="PKV97_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNB06786.1"
                     /translation="MLNEDSTLLAGLAASAFLAATLLPGGSEAVFAALLALRPELTPA
                     ALLVATAANTAGGMSTYALGRLLPRKESGAHARTLLRWGSLALLLSWVPLIGDALCAA
                     AGALRLNAFACLAWMALGKGLRYAAIAWALPSAGA"
     gene            3446..3796
                     /locus_tag="PKV97_12710"
     CDS             3446..3796
                     /locus_tag="PKV97_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNB06787.1"
                     /translation="MSAIERHGVTARYADAVIHAGVLHLVEVPASSAGSIEAQTREVL
                     ESVGRQLAKLGSDPARILMATIYLVDMADYAGMNAVWDAWVPPGSAPARACVQVAGLA
                     DPGWRIEIALTAAR"
     gene            3921..>4389
                     /locus_tag="PKV97_12715"
     CDS             3921..>4389
                     /locus_tag="PKV97_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HNB06788.1"
                     /translation="MSQRLREIPYNYTSFSDREIVIRLLGAEAWAVLDQLRTERVTGR
                     SARMLYEVLGDIWVVRRNPYLEDDLLTSRERRDALIGALDHRLNEIDKRRQGNDHVTL
                     LVARAREAVSNFERWFDDTARRRKAVLKALARHTRRDNICFDGHARVSHVTDAT"
BASE COUNT          660 a         1645 c         1426 g          658 t
ORIGIN      
        1 gcgatcgagc tcgcccccgc ggtgcgggtc aacggcgtct cgcccggccc gatcgaatgg
       61 ccggaggacg accagttccc gcccgccgag cgcgagcgca tcgtcgccca caccttgctc
      121 ggccgcgtcg gcagcccgga cgacatcgcg cgcacggtcg ccttcctgat gttcgacgca
      181 ccctacatca ccggccagat cctcgcagtc gatggcgggc gcagcgcgca tctgtagcca
      241 agggtcgttt ccgcgccggt ttctgggata attcgcacct gattttcccg acgagcatcg
      301 accgtgaccg cagcctccgc ccccgccgcc gccctcccca ccaccgaggc cggcggagac
      361 gcccgcttct ccaacacctt cctgcgcctg aagaagaaac tcgagcgtgg cgtcggcgag
      421 gccatcggcg actacaacat gatcggcgac ggcgacaccg tgatggtgtg cgtctccggc
      481 ggaaaggact cctacaccct gctgtcctgc ctgctcgcgc tgcgcgaacg cgccccggtc
      541 gacttccgca tcgtggcgat gaacctcgat cagaagcagc ccggcttccc cgaccacgtg
      601 ctgccggcct acttcgagtc gatcggtgtc gagtatcgca tcgtcaccga ggacacctac
      661 tcgatcgtca aggacaagat tcccgagggc aagaccacct gctcgctgtg ttcgcgtctg
      721 cgccgcggca tcatctaccg caccgccacg gagatcggcg ccacccgcat cgcgctcggc
      781 caccaccgcg acgacatgct cgagaccctg ttcctcaaca tgttcttcgg cggcaagatc
      841 aaggccatgc cgcccaagct ggtcagcgac gacggcaagc acgtggtgat ccgcccgctc
      901 gcctactgca ccgaggccga catcgcacgc ttcgcgcgca cgatggactt ccccatcatc
      961 ccgtgcaatc tgtgcggctc gcaggaaaac gcccagcgca agctgatcaa gaacatgctg
     1021 cagggctggg cgcgcgacta cccgggccgc atcgaatcgc tcgccacttc gctgaagaac
     1081 gtggtgccct cgcacctgtc cgacgcgcgc ctgttcgact tcgtcggcct cacgcagcac
     1141 accgtggtcg aagaaggcga caccgccttc gatccgcccg agctgcccga ccgtacgctc
     1201 ggcgccagcg tgaagctgtt ccgcgcgggc gaccaggaag aacgcgaatg cgcctgaggg
     1261 aggcgcgacc gcgcctgatt cggatgccga cgccggcgcc ttgcggatcc ggtgcgacat
     1321 ccccatgatc ctgttccggg ccggccacgc cagccgccag atctgacacg gaaaaccctg
     1381 cgatgcccga gcgaaagaac ctctgcgtgc tggtcgcgct ccatccggtg gatgcggtgc
     1441 tcgccgaacg cctcggtgcg gacgaggcgc gccatgccgc cgagcgtcac gcgcggcgga
     1501 tcgagcgtgc gatcgtgatg cagggcggcc aggtcctgct cggcggcgcg gagcagacca
     1561 tcgccggatt cgaacgcagc gacgcggcgg tgcacgcggc gagcgaggcg ctcgaccgca
     1621 tacacggcct tccacccctg ctcggcagca agcagacggt acgcatcggc gtgcactatg
     1681 gggtcctgga caacgatggc gaggagcccg ccggagaggg tccagcggtg gcccgccagc
     1741 tcgcccatct ggccgagagc gggcaggcgc tgatctccgg caccgcggtg atggtcctcg
     1801 gcggtgccgc acggcaacag gtcggctcgc agccgctgag cggtccggtg tggacgcggg
     1861 tcggcatccc ggtgcatgcg atcggacagc gcagcggtct ggtcacctcg gttcccggcg
     1921 cagcgcggct gtcgagccgg ctgcgcctgc gccaccagca ggaagtcctg ttcgtggagg
     1981 agttgcgccc ggtgctgctg ctcggccgcg agctggccaa cgacgtcacg atcatggatc
     2041 cgcgcgcctc gcgtcagcat gcgcgcatcg agcgccgccc cgagggcttc gtgctgttcg
     2101 atcagagcac caacggcagc ttcgtcgcca tcgagggcaa gccggagtgc ttcgtgagac
     2161 aggagcagat gctgctcgac ggtcccggcc ggctcggctg cggcttctcg acgaccgagc
     2221 tcgagcgcga cctggtgttc ttcgacatcg tctgagcccc gcgcgcggga gaagggcgtg
     2281 cgcgggggac acaggcgccc gcccgcgcgg cacgcccgca tggaaacgac cggatcagcc
     2341 gctcatgcat tcgccgatga aggccttgcg cttggccccg cgcagcgact gctcggtggc
     2401 cgcacgattg caggccttct gccttgccat gtcctcgctc gagaccttct gtgcggagcc
     2461 cgctgtcggc gcgaccgcaa gcgcaggtgc ggctgcaccg gccgcgctcg cggccgccgg
     2521 tgcgaccgcc gaggccgctg ccggcaccat agccggtgcc gcaacaggcg ctgcagccgg
     2581 gacgccggcc gcgctgccgg aggcgagagc aggcgcggca cccggagcac tcgcagcggc
     2641 agcctcgtgc ttgccgctca ggcaggtgct catgaaggcc ttgcgcgcat cgcccccgag
     2701 cgcctgcgtc ttcgcttcgg cattgcaacg cttcatgcgt tcgtgctggg gattggccca
     2761 tgcctgcgcg ggggtcagcg ccgcgagcac gagaacgacg ccggtggcgc cgagaattcg
     2821 cttcatgatc ttgtctcctc gtccggttct acgcaaaatg cgtaacacga ttttgcatat
     2881 gacatacatc ggcgtcaatg cgcatcgcat tgccaccgag acgcatgcgg ctccccgccg
     2941 gcgcccgcac cgatgctgaa cgaggactcc acgctcctcg cgggcctcgc cgcgtccgcc
     3001 ttcctggccg cgaccctgct gccgggcggc tccgaagcgg tattcgccgc gctgctggcg
     3061 ctgcgcccgg aactgacgcc ggcagcgctg ctcgtcgcca ccgcggccaa caccgcgggc
     3121 ggcatgagca cctacgcgct cggccgcctg ctgccccgca aggaatccgg cgcgcatgca
     3181 cgcacgctgc tgcgctgggg cagcctcgcc ctgctgctgt cctgggtgcc gctgatcggc
     3241 gacgcgctgt gcgctgccgc gggtgcgctg cgcctgaacg ccttcgcctg cctggcgtgg
     3301 atggcgctcg gcaagggcct gcgctacgcg gcgatcgcct gggcattgcc gtccgccggc
     3361 gcctgagccc gccgcgcacg ccgacccgcg ccgcgccgcc gccctcgcct atactcgccc
     3421 cctgccctcg caacggagcc cccccatgtc cgccatcgaa cgccacggcg tcaccgcccg
     3481 ctatgccgac gccgtcatcc acgccggcgt cctgcacctg gtcgaggtcc ccgcgagcag
     3541 cgccggatcg atcgaagccc agacacgcga ggtgctggag tccgtcggcc gccagctcgc
     3601 caagctcggc agcgaccccg cgcgcatcct gatggccacc atctacctcg tcgacatggc
     3661 cgactacgcc ggcatgaacg cggtgtggga tgcctgggtt ccgcccggca gcgccccggc
     3721 acgcgcctgc gtccaggtcg ccgggctcgc cgacccgggc tggcggatcg agatcgcgct
     3781 caccgccgcg cgctgaagct cgccgcgccg ggcgacggag gccgaatccc tgctggcctt
     3841 aaggtcttga tttttgctgc atggtgcact gcggttcgct agaatggacc gcttccctgc
     3901 agccgcttcc gggtagtcca atgagccaac gcctgcgcga gattccgtac aactacacct
     3961 cgttttccga ccgcgagatc gtcatccggc tgctcggggc cgaggcctgg gcggtgctcg
     4021 accagctccg caccgaacgc gtcaccggcc gctcggcgcg gatgctctac gaggtcctcg
     4081 gcgacatctg ggtggtgcgc cgcaacccct acctcgagga cgacctcctc acctcgcgcg
     4141 agcgccgcga cgcgctgatc ggcgcgctcg accaccgcct caacgagatc gacaaacgcc
     4201 gccagggcaa cgaccacgtc accctgctgg tggcgcgtgc gcgcgaggcg gtgtccaact
     4261 tcgagcgctg gttcgacgac accgcgcgcc gccgcaaggc ggtgctcaag gcgctggcca
     4321 ggcacacccg gcgcgacaac atctgcttcg acggtcacgc ccgcgtctcc cacgtcaccg
     4381 acgccaccg
//
LOCUS       DAOQNN010000271         4372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_12575_length_4372_cov_9.686820, whole genome shotgun sequence.
ACCESSION   DAOQNN010000271 DAOQNN010000000
VERSION     DAOQNN010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12575_length_4372_cov_9.686820"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /gene="prpE"
                     /locus_tag="PKV97_12720"
     CDS             complement(<1..1496)
                     /gene="prpE"
                     /locus_tag="PKV97_12720"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HNB06789.1"
                     /translation="MTDYQQFHRRSIEDRDGFWAEQAQLVHWSKPAQQVCDYSNPPFV
                     KWFKGGETNLCYNAVDRHAAARPNDRALVYISTETDEEKVYSFAELQREVERMAAIYQ
                     ELGVKRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFAAGSLATRIDDAKPVLM
                     VSSDAGMRNGKPVPYKHLVDEACKLAEFPPAKVLIVDRGLDKGFPKVEGRDVDYAELR
                     ARHLDAKVPVVWLESSEPSYILYTSGTTGKPKGVQRDTGGYTVALTASMKHIFTGGAG
                     ETMFSTSDIGWVVGHSYIIYGPLLAGMATIMYEGTPLRPDAGIWWQIVEKYKVTVMFS
                     APTAVRVLKKQDPAFLKKYDLSSLKHLFLAGEPLDETSHQWIMEELGIPVIDNYWQTE
                     TGWPMLAICRGVEDSPIKLGSPAFPVYGYDLRIFREDGTECGANEKGIVGIVPPLPPG
                     CLSTVWGQDDRFVSTYFSLFTEPVVYSSSDWGIKDDKGYYTILGRMDDV"
     gene            complement(1633..2808)
                     /locus_tag="PKV97_12725"
     CDS             complement(1633..2808)
                     /locus_tag="PKV97_12725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB06790.1"
                     /translation="MDPGALTTDGDGRLPRILVVDDSRMVRASIVKHIRGRFEAREEP
                     DGEAAWEALLVDPAIEIVLTDIGMPRLDGYGLLERLRASKLPRVRDLPVVIISGDEDE
                     SARARALALGANGFVTKGTGGVELLATLMSLLRLSKAQGELERSRAALERHSPVDPES
                     GLATEAYLEHHGAQAVAEARRRQADIGAMVIEIDQFDELATRYGVQVVQLILRKLGKL
                     VSGRIRAEDTLSQHGQACFALLSPGIDRVSTCAFALRLRASVEKLVMAYRDERIRVTL
                     SIGVASAVTDGREAIGALLALAQERATQAHTAGGNRVVAEGGEVDPAAIERELARVPS
                     VDGALRLLRAGARDEVAGQLPALVGALLPLLELLESRLQSGLPLARLREFHDKGGGG"
     gene            complement(2822..3817)
                     /locus_tag="PKV97_12730"
     CDS             complement(2822..3817)
                     /locus_tag="PKV97_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNB06791.1"
                     /translation="MNPFKAFVIDQDENRKVVGGMRTMSADQLDAGEVTIRVHYSSIN
                     YKDALAATGAGKIIRRFPCVGGIDLCGEVVDSADARFRPGDKVIATSFDIGVAHHGGY
                     AEYARVPAGWVVPLPAGLDLFEAMALGTAGFTAALGIVRMEDNGLAPANGPVVVTGAT
                     GGVGALAIDMLAQLGYHVVALTGKDAEADYLKMLGAAEILPRSSIDFDKVRPLEAARW
                     AGAVDNVGGQILHWVLATMKQAGTVASIGNAASFNVDTTVFPLILRGVSLLGVDSGYM
                     GFPTRQRVWERLATDLKPRHLAAITRTIDFDALPGAFDDFIHGRVKGRTVVRIGA"
     gene            4002..>4372
                     /locus_tag="PKV97_12735"
     CDS             4002..>4372
                     /locus_tag="PKV97_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB06792.1"
                     /translation="MPLLPSPLPSSAAYIDAFTRAGLPVLRHTVRAFEELRSADPAPG
                     AREIAAVVLADPLMTLRLLTHLEANRRASQNHDITTIERAVLMLGVEPFFATFCDLPT
                     VEDTLAAHPKALVVALKLIARA"
BASE COUNT          648 a         1528 c         1505 g          691 t
ORIGIN      
        1 acgtcgtcca tgcggccgag gatggtgtag tagcccttgt cgtccttgat gccccagtcg
       61 gacgaggaat acaccaccgg ctcggtgaac aggctgaagt aggtgctgac gaagcggtcg
      121 tcctggcccc acacggtgga caggcagccc ggcggcagcg gcggcacgat gccgacgatg
      181 cctttctcgt tggcgccgca ctcggtgccg tcctcgcgga agatgcgcag gtcgtagcca
      241 tagaccggga aggcgggcga gccgagcttg atcgggctgt cttccacgcc gcggcagatg
      301 gcgagcatcg gccagccggt ctcggtctgc cagtagttgt cgatgaccgg gatgccgagc
      361 tcttccatga tccactggtg gctggtctcg tcgagcggct cgccggcgag gaacaggtgc
      421 ttgagcgagg acaggtcgta cttcttgagg aaggccgggt cctgcttctt gagcacgcgc
      481 accgcggtgg gcgcggagaa catcaccgtg accttgtact tctcgacgat ctgccaccag
      541 atgccggcgt cggggcgcag cggcgtgccc tcgtacatga tcgtggccat gccggcgagc
      601 agcgggccgt agatgatgta gctgtggccg accacccagc cgatgtccga ggtcgagaac
      661 atggtctcgc ccgcgccgcc ggtgaagatg tgcttcatcg acgcggtcag ggccacggtg
      721 tagccgccgg tgtcgcgctg cacgcccttg ggcttgccgg tggtgccgga ggtgtacagg
      781 atgtagctcg gctcggagga ctcgagccac acgaccggga ccttggcgtc caggtgcctg
      841 gcacgcagct cggcgtagtc gacgtcacgg ccttccacct tggggaagcc cttgtccagg
      901 ccgcgatcga cgatcagcac cttggcgggc gggaattcgg cgagcttgca ggcttcatcc
      961 accaggtgct tgtagggcac cggcttgccg ttgcgcatgc cggcatccga gctgaccatc
     1021 agcaccggct tggcgtcgtc gatgcgggtg gcgagcgagc cggccgcgaa accgccgaac
     1081 accaccgagt ggatcgcgcc gatgcgcgcg caggccagca tggcgaacgc ggcctcggcg
     1141 atcatcggca tgtagatcag cacgcgatcg ccgcgcttga cgccgagctc ctggtagatc
     1201 gccgccatgc gctcgacctc gcgctgcagc tcggcgaagg agtagacctt ctcctcgtcg
     1261 gtctcggtcg agatatacac cagcgcacgg tcgttcgggc gcgcggcggc gtggcgatcg
     1321 acggcgttgt agcacaggtt ggtctcgcca cccttgaacc acttgacgaa cggcgggttg
     1381 gaatagtcgc agacctgctg cgcgggcttg ctccagtgca cgagctgcgc ctgctcggcc
     1441 cagaacccgt cgcggtcttc gatggaacgg cggtggaact gctggtaatc ggtcattgct
     1501 tcctctccct tctgccttca atgatcgtgt tgtcgaactc gacgtgccac cggcagcgcg
     1561 gcgccgggac agcgcagcga agcccgcggg cacacctgtt ggggacggcc tgcgggtgcg
     1621 gccgccctcc actcatccgc ccccgccctt gtcgtggaac tcgcggagtc ttgcgagcgg
     1681 taatccggat tgaagcctgg attctagcaa ttcgagcagc ggcagcagcg cgccgacgag
     1741 cgcgggcagc tgccccgcca cctcgtcgcg cgcgccggcg cgcagcaggc gcagcgcgcc
     1801 atcgacgctg gggacgcgcg caagctcgcg ttcgatcgcc gccgggtcga cctcgccgcc
     1861 ctcggcgacg acgcggttgc cacccgccgt gtgcgcctgc gtggcgcgct cctgggcgag
     1921 cgcgagcagc gcgccgatgg cctcgcgacc gtcggtgacc gcgctggcga cgccgatgct
     1981 cagggtcacg cggatgcgct cgtcgcggta ggccatcacc agcttctcga cgctggcgcg
     2041 caggcgcagc gcgaaggcgc aggtggacac gcggtcgatg cccggcgaca gcagcgcgaa
     2101 gcaggcctgg ccgtgctgcg acaaggtgtc ctcggcgcgg atgcgcccgc tcaccagctt
     2161 gccgagcttg cgcaggatga gctgcaccac ctgcacgccg tagcgcgtgg cgagctcgtc
     2221 gaactggtcg atctcgatca ccatcgcgcc gatgtcggcc tggcgccggc gcgcctcggc
     2281 cacggcctgg gcgccgtgat gctcgaggta ggcctcggtc gccaggcccg actcggggtc
     2341 gaccgggctg tggcgctcga gcgcggcgcg gctgcgctcg agctcgccct gcgccttgga
     2401 caggcgcagc agcgacatca gcgtggcgag cagctcgacc ccgcccgtgc ccttggtgac
     2461 gaagccgttg gcgccgaggg cgagcgcgcg cgcacgcgcg gactcgtcct cgtcgccgga
     2521 gatgatcacc accggcaggt cgcgcacgcg cggcagcttc gaggcgcgca ggcgctcgag
     2581 caggccgtag ccgtccaggc gcggcatgcc gatgtcggtg agcacgatct cgatcgccgg
     2641 atcgaccagc agcgcctccc aggcggcctc gccgtcgggc tcctcgcgcg cctcgaaacg
     2701 accgcggatg tgcttgacga tggacgcgcg caccatgcgc gagtcgtcca cgacgaggat
     2761 gcgcggcagc ctgccgtccc cgtccgtcgt cagcgcgccc gggtccatgg cgttcttcgg
     2821 ctcaggcgcc gatgcgcacg acggtgcgcc ccttgacgcg gccgtggatg aagtcgtcga
     2881 aggcgcccgg caacgcgtcg aagtcgatcg tgcgcgtgat cgcggcgagg tggcgcggct
     2941 tgaggtcggt ggccaggcgc tcccacacgc gctgccgggt cgggaagccc atgtagcccg
     3001 aatccacccc gagcaggctc accccgcgca ggatgagcgg gaacaccgtg gtatcgacgt
     3061 tgaagctcgc cgcgttgccg atgctcgcca ccgtgccggc ctgcttcatg gtcgccagca
     3121 cccagtgcag gatctgcccg ccgacgttat ccaccgcgcc ggcccagcgc gccgcctcga
     3181 gcggtcgcac cttgtcgaag tcgatggagg agcgcggcag gatctccgcc gcgccgagca
     3241 tcttcaggta gtcggcctcg gcatccttgc cggtgagcgc gaccacgtgg taaccgagct
     3301 gcgccagcat gtcgatggcc agcgcgccga cgccacccgt ggcgccggtg accaccaccg
     3361 gcccgttggc cggggcgagg ccgttgtcct ccatgcgcac gatgccgagc gcggcggtga
     3421 agcccgcggt gcccagcgcc atcgcctcga agagatcgag ccccgccggc aagggcacga
     3481 cccagcccgc cggcacgcgc gcgtactcgg catagccgcc atggtgggcg accccgatgt
     3541 cgaagctggt ggcgatcacc ttgtcgcccg gcctgaagcg cgcatcggcg ctgtcgacca
     3601 cttcgccgca caggtcgatg ccgccgacgc aggggaagcg acggatgatc ttgccggcgc
     3661 cggtcgcagc gagcgcgtcc ttgtagttga tgctggagta gtggacgcgg atcgtcacct
     3721 cgcccgcatc gagctggtcg gcgctcatcg tgcgcatgcc gccgaccacc ttgcggttct
     3781 cgtcctggtc gatcacgaag gccttgaagg gattcatgcg gaagtctcct gtcgatgtgg
     3841 cggccagcct ggaggctgcg ttgattcata attatagccg caactgcgaa tctgcccgaa
     3901 cacggcacgc tcgcctcaag tttgtgccgc ggcggccgtc atcccggttt gattccatga
     3961 gccgcaccac cgccaccccg accgcgccag agaccgcgaa catgcccctg ctgcccagcc
     4021 ccctgccctc ctccgcagcc tacatcgacg ccttcacccg cgccggactg cccgtcctgc
     4081 gccacaccgt acgcgccttc gaggaactgc gcagcgcgga tcctgcgccg ggcgcacgcg
     4141 agatcgccgc ggtggtgctg gccgatccgc tgatgacctt gcgcctgctc acgcatctcg
     4201 aggcgaaccg gcgcgcctcg cagaaccacg acatcaccac catcgagcgc gcggtgctga
     4261 tgctgggcgt ggagccgttc ttcgccacct tctgcgatct tcccacggtg gaagacaccc
     4321 tcgcggcgca cccgaaggcg ctcgtcgtgg cgctcaagct gatcgcgcgc gc
//
LOCUS       DAOQNN010000272         4353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_12649_length_4353_cov_7.784784, whole genome shotgun sequence.
ACCESSION   DAOQNN010000272 DAOQNN010000000
VERSION     DAOQNN010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12649_length_4353_cov_7.784784"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..2304
                     /locus_tag="PKV97_12740"
     CDS             277..2304
                     /locus_tag="PKV97_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HNB06793.1"
                     /translation="MEKLLERFGQPDQDPVRLWSALVDKLRPARPTDTDQATGNLREL
                     GNLLARRPDLLANLRTAVLELFAERKQVTMYVSSGLLPSTGFFSETSRRFGSRMLPEL
                     LDTTHLKDLLSAVFHRVDDEVWVRAIADEAWQDFLRLLVGHQTPMFEEDASPLPSAVG
                     EILESLRVLSFHVSAIGLDRELVRIDPHLEEHESPFLAQNAELLAYIGHYKAWWTTPG
                     ALIADDKHLTVMLHQCDEVLQRVRKRAMRVGTSLTLTFKLERLRQHLERIHELIALLG
                     ELRIRRVVEDAAPRIVRLFKTLVRAECRKNILSDYWGQNVELLSLRMTESASRTGEHY
                     ITSSRSEYFGLFASAALGGLIIAFMAGNKVVLGSQGMAPLNELLSFCLNYGLGFALIH
                     MLGGTVATKQPAMTANAIAASIGEARGKTRDLEALADLIVRTIRSQIGAILGNIGVAI
                     PVAIGVGVLIHLATGSHFISPEKAHSLLAEIHPLGGALFFAAIAGVCLFMSGLIAAYY
                     DNLSAYNRIPQRLTQLRGLRRLLGERRLRKLADYVEQNIGALVGNFFFGFLLGGATGL
                     GVLFGLPIDIRHIAFSSAYLGYAAAALDFSIPLTTAAIAFAGVLLIGLTNLTVSFALT
                     LSVAMRARRITFAQSRSLGGLLLRRLLRTPHAFLFPPRGDEPALGPTPPNG"
     gene            2323..2397
                     /locus_tag="PKV97_12745"
     tRNA            2323..2397
                     /locus_tag="PKV97_12745"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2356..2358,aa:Arg,seq:cct)
     gene            2497..3156
                     /locus_tag="PKV97_12750"
     CDS             2497..3156
                     /locus_tag="PKV97_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNB06794.1"
                     /translation="MPRYSQVLFDLDGTLIDSAPAILASYRDAFASTGHQPVIAIDAG
                     IVGPPLLETLRMLAGTTDIGVIDALAAGFKASYDSRGYRQTAAYAGVDAMLRRLGAAG
                     CTLSIATNKRLLPTRLILEHLGWSGYFAAVYALDLFEPRLPDKASMIARLLQDRGIAR
                     GDAVYVGDRSEDGESADANRLPFIAATWGYGSLESTEMAPHWRAVASPAALVDAILGD
                     A"
     gene            complement(3182..3979)
                     /locus_tag="PKV97_12755"
     CDS             complement(3182..3979)
                     /locus_tag="PKV97_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNB06795.1"
                     /translation="MNPRPETRARRRLLACALALPAALALAPIARAELASTLARVKPS
                     VVAVGTYQRTRSPAFQFRGTGFAVGDGMLVATNAHVLPDEIDGAKMEALVIVLPGDDL
                     TGAVRPVKVVATERAQDLALLRLEGGPPLPALRLAGGGGVVEGQEVAFTGFPIGAVLG
                     MTPVTHRGIVSAITPIAIPQANSRDLNPALIRRLATDPFRVYQLDATAYPGNSGSPLY
                     DPASGEVIGVLNMVFVKSTKEKVLADPSGISYAIPVEYLQRLIAGRN"
     gene            complement(3976..>4353)
                     /locus_tag="PKV97_12760"
     CDS             complement(3976..>4353)
                     /locus_tag="PKV97_12760"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNB06796.1"
                     /translation="SALNPDALSPKNAMGVMQLIPATAERFNVGKPYDPEQNIRGGLA
                     YLRWLLAYFEGDIALAAAAYNAGEGAVDRYRGVPPYRETRAYVERILRHVGQDRHPYD
                     SRVVPPSPMLRKLRLALEEEHES"
BASE COUNT          662 a         1542 c         1475 g          674 t
ORIGIN      
        1 acgcgctccc cggcgtcgag ctggaagctc acgtgatcga gcaggtcgac gtgaccgaag
       61 gcgaggcagg cgttatctac ggcgaggagc ggcatcttgg cgggcacggc gcgcgggccg
      121 cgggtcggat cggggtggcg attctagccc cgcgctggcg gctccggcgg aatcccgcat
      181 aatgcagtgc acaaaaccgc cgcgcggcga cccggttcag cgcgcgacgg tcgcgcctgg
      241 cgcggccgcg aggcggatac ggaacacggg aaacgaatgg aaaaactgct cgaacgcttc
      301 ggccagccgg accaggatcc ggtccggctg tggtccgcac tggtcgacaa gctgcgcccc
      361 gcgcgcccga ccgacaccga ccaggccacc gggaacctgc gcgaactcgg caacctgctc
      421 gcgcgccggc ccgatctgct ggcgaacctg cgcacggcgg tgctggagct cttcgccgag
      481 cgcaagcagg tgacgatgta cgtctcctcc ggcctcctgc cctcgaccgg gttcttctcc
      541 gagacctcgc gcaggttcgg cagccgcatg ctgcccgagc tgctcgacac cacccacctc
      601 aaggacctcc tctccgcggt cttccaccgc gtggatgacg aagtctgggt gcgcgccatc
      661 gcggacgagg cctggcagga cttcctccgc ctgctggtcg gacaccagac cccgatgttc
      721 gaggaagacg ccagcccgct gccgagcgcg gtgggcgaga tcctcgagtc gctgcgcgtg
      781 ctctccttcc acgtctcggc gatcgggctc gaccgcgagc tggtgcgcat cgatccccac
      841 ctcgaggagc acgaatcccc cttcctcgcg cagaacgccg agctgctcgc ctacatcggc
      901 cactacaagg cgtggtggac caccccgggc gccctgatcg ccgacgacaa gcacctcacc
      961 gtgatgctgc accagtgcga cgaggtgctg cagcgggtgc gcaagcgcgc gatgcgggtg
     1021 ggcaccagcc tgacgctgac cttcaagctc gagcgcctgc gccagcacct ggagcgcatc
     1081 cacgagctca tcgcgctgct cggcgagctg cgcatccggc gcgtggtcga ggacgccgcg
     1141 ccccgcatcg tgcgcttgtt caagaccctg gtgcgcgccg agtgccgcaa gaacatcctc
     1201 tccgactact ggggccagaa cgtcgagctg ctgtcgctgc gcatgaccga gagcgcgagc
     1261 cgcaccggcg agcactacat caccagttcg cgcagcgagt atttcgggct gttcgcatcg
     1321 gccgcgctcg gcgggctgat catcgccttc atggctggca acaaggtcgt gctcggcagc
     1381 cagggcatgg ccccgctcaa cgagctgctg tccttctgcc tcaactacgg actgggcttc
     1441 gcgctgatcc acatgctcgg cggcacggtc gccaccaagc agccggcgat gaccgcaaac
     1501 gcgatcgccg cctcgatcgg cgaggccagg ggcaagacgc gcgacctgga ggcgctcgcc
     1561 gacctgatcg tgcgcacgat ccgcagccag atcggtgcca tcctcggcaa catcggggtg
     1621 gcgatcccgg tggcgatcgg cgtgggcgtg ctgatccacc tcgccaccgg cagccatttc
     1681 atcagcccgg agaaggcgca ctcattgctg gccgagatcc atccactcgg cggcgcgctg
     1741 ttcttcgccg cgatcgcggg cgtgtgcctg ttcatgtcgg ggctgatcgc cgcctattac
     1801 gacaacctct cggcctacaa ccgcatcccg cagcggctca cccagctgcg cggcctgcgc
     1861 cgcctgctcg gcgagcgccg cctgcgcaag ctcgcggact acgtcgagca gaacatcggt
     1921 gccctcgtcg gcaacttctt cttcggtttc ctgctcggcg gcgccaccgg cctgggggta
     1981 ctgttcggcc tgccgatcga catccgccac atcgccttct cgtcggccta cctcggctac
     2041 gccgcggcgg cgctcgactt ctcgatcccg ctgacgaccg cggcgatcgc cttcgccggc
     2101 gtgctgctga tcggcctcac caacctgacg gtgagcttcg cgctgacgct gagcgtggcg
     2161 atgcgcgcac gccgcatcac cttcgcgcag agccgctcgc tcggcggcct gctgctacgc
     2221 cgtctgctgc gcacgccgca cgccttcctg ttcccgccgc gcggggacga gccggcactt
     2281 ggtccgacgc cgccgaacgg ctagaatgcg cgtccccgag tcctcgtcat agttcaatgg
     2341 atagaacggg cgcctcctaa gcgccagatc caggttcgat tcctggtgac gggaccactc
     2401 attcgcgttt tcggtgttgt aaacggttgg tcgcggcttg ctcggcaggc gctcgattcg
     2461 gggaccggag gcgtgcttcc cgatcgcacg agcccgatgc cccgctactc ccaggtgctg
     2521 ttcgacctcg acggcaccct gatcgactcc gcccccgcca tcctcgcgag ctaccgcgac
     2581 gccttcgcca gcacgggtca ccagccggtg atcgcgatcg acgcgggcat cgtcggcccg
     2641 ccgctgctcg agaccctgcg catgctcgcc gggaccaccg acatcggggt gatcgacgcg
     2701 ctcgcagccg gctttaaggc gagctacgac agccgcggct accgccagac cgcggcctac
     2761 gccggcgtcg acgcgatgct gcgccgcctc ggcgcggccg gctgcacgct gtcgatcgcg
     2821 accaacaagc gcctgctgcc gacgcggctg atcctcgagc acctgggctg gagcgggtat
     2881 ttcgccgcgg tgtatgcgct cgacctgttc gagccacgcc tgcccgacaa ggcctcgatg
     2941 atcgcgcgcc tgctgcagga tcgcggcatc gcccgtggcg atgcggtcta tgtgggcgac
     3001 cgcagcgagg acggcgaatc ggccgacgcc aaccggctgc ccttcatcgc cgcgacctgg
     3061 ggttacggca gcctggagtc gaccgagatg gcgccgcact ggcgcgcggt ggccagcccg
     3121 gcggcgctcg tcgacgcgat tctgggcgac gcctgacgct tgcgggcgtg ctcgtgcgcg
     3181 cctagttccg gccggcgatc aggcgctgca ggtactcgac ggggatggcg tagctgatgc
     3241 ccgacggatc ggcgagcacc ttttccttgg tcgacttcac gaacaccatg ttgagcacgc
     3301 cgatcacctc gccactggcg ggatcgtaga gcgggctgcc actgttgccc ggataggcgg
     3361 tggcgtcgag ctggtagacg cggaacggat cggtggcgag acgccggatg agcgccgggt
     3421 tgagatcgcg agaattggcc tgaggaatcg cgatcggcgt gatcgccgag acgatgccgc
     3481 ggtgcgtcac cggggtcatg ccgagcacgg caccgatcgg gaagccggtg aaggcgacct
     3541 cctggccctc gaccacgccg cccccgccgg cgagacgaag cgcaggcagc ggcggcccgc
     3601 cttcgagccg gagcaaggcg agatcctgcg cgcgctccgt ggccaccacc ttcaccggtc
     3661 gcaccgctcc ggtgaggtcg tcgcccggaa gcacgatgac gagcgcctcc atcttcgcgc
     3721 cgtcgatctc gtcaggcagg acatgggcgt tggtggcgac cagcatgccg tcgccgaccg
     3781 caaagccggt accacggaac tggaaggccg ggctgcgtgt acgctgatag gtaccgacgg
     3841 cgacgaccga gggcttgacg cgcgccagcg tggacgccag ctcggcacgt gcgatcgggg
     3901 ccagggcgag cgcggcggga agggcgagcg cgcaggccag cagccgacgc cgcgcacgcg
     3961 tctccgggcg gggattcatg actcgtgttc ctcttccaga gcgaggcgga gcttgcgcaa
     4021 catcgggctg ggggggacca cgcggctgtc gtagggatgg cgatcctgac cgacgtgacg
     4081 caggatgcgc tccacgtagg cacgcgtctc gcgatacggc ggcacccccc gatagcggtc
     4141 gaccgccccc tctccggcgt tgtaggccgc tgcggcgagc gcgatgtcgc cctcgaaata
     4201 ggccagcagc cagcgcaggt aggcgaggcc gccacggatg ttctgctcgg gatcgtaggg
     4261 cttgccgacg ttgaagcgct cggccgtggc gggaatgagc tgcatgacgc ccatcgcgtt
     4321 cttgggcgag agcgcgtcgg ggttcagggc cga
//
LOCUS       DAOQNN010000273         4310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_12866_length_4310_cov_10.177673, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000273 DAOQNN010000000
VERSION     DAOQNN010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12866_length_4310_cov_10.177673"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..753)
                     /locus_tag="PKV97_12765"
     CDS             complement(253..753)
                     /locus_tag="PKV97_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HNB06797.1"
                     /translation="MRSRRRFLATSLAAGVLAAHRSAFAHGKAEHPHKAASDAPKEQQ
                     EWGIAGDERSVARTIPIVMTDAMRFVPDRIAIRLGETIRFTHENRGSVMHEMVVGTPA
                     TLAEHAELMRRFPGMEHDEPWMAHVGPGGRGQMIWHFNRAGDFQFACLIPGHFQAGMI
                     GTITVG"
     gene            complement(914..1378)
                     /locus_tag="PKV97_12770"
     CDS             complement(914..1378)
                     /locus_tag="PKV97_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06798.1"
                     /translation="MKLRSFRLAGLMPVAALVAALHGSPVLAQTTEQAQPWPPAASGP
                     ALQARMKSIRAETDPERRMALMEEQIKALEAASQGTPAVCPMMGGQMQGGPMGAGPMM
                     KGAGAGPGAGGMGGMMMDPRLVQEHMKMMQEHMGMMQNMMKMHPAVKPAMPN"
     gene            1565..2245
                     /locus_tag="PKV97_12775"
     CDS             1565..2245
                     /locus_tag="PKV97_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HNB06799.1"
                     /translation="MKILIVEDEVKTGDYLRQGLTEAGFVVDLARDGLDGLHLALSGD
                     YDLIVLDVMLPSLDGWGILQTLRRSGRENPVLFLTARDQIEDRVRGLELGADDYLVKP
                     FAFSEFLARVRTLLRRGRSHEPDTLRSANLELDLLRRRVTRCGQRIDLTAKEFALLEL
                     LLRRKGEVLPRSLIASQVWDMNFDSDTNVIEVAVRRLRVKVDDPFEPKLIRTVRGMGY
                     VLEAMEPS"
     gene            2248..3651
                     /locus_tag="PKV97_12780"
     CDS             2248..3651
                     /locus_tag="PKV97_12780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HNB06800.1"
                     /translation="MIRIHSLTARLATLFAVLAASLLVLVAVLFGRMLDAHFQELDMH
                     ELQGKVTLIRNTLQDEAASGGRAERIQALERSFVGHESVGVLLRDVDGRVLNVIHPEH
                     FTAAQRAGERPSGVVTDWTVDGRPHRGVEVGIALPRSGSKDQTIAALVALDLSHHVHF
                     LASVRNATWAGVVAAAMAAALFGWFAAHRGLAPLRRVTETARRLSARQLAERLSIDDA
                     PLEVRDHAEAFNGMLARLEAAFQRLGDYSADIAHELRTPISNLMTQTQVALSRPRTID
                     EYQDILASNLEEYERIARMVSDMLFLAKAEENTLAHASEPVDLALEADALIDFYEALA
                     EERQVRIVREGQASVQGDRLMLRRALSNLISNALRHTPDEGQITIRIDADATDIRLAV
                     CNVGDPIPGDQIERIFERFHRGSAQREFRGEGAGLGLAITRSIVQAHGGQISAQSAEG
                     ATCFTITLPGHRAPSSS"
     gene            4113..>4310
                     /locus_tag="PKV97_12785"
     CDS             4113..>4310
                     /locus_tag="PKV97_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06801.1"
                     /translation="MTILTPAQRRATCIHEAGHAVVFALGGVSVYRLAVAAAGAEAWR
                     TDIRNGRMCTGLWGLCEKADLV"
BASE COUNT          710 a         1456 c         1398 g          746 t
ORIGIN      
        1 cttgaccgta ccctcggcca gcttggcatc ggcctcgctc gcggcactcg ccccgccgtg
       61 atgggcactg tggtcggcct gcgcaaacgc taccggggca gcgcccccca gcacggccag
      121 gagtgcagtg gtgatgaggg tcttcttcat gatgggtttc tccaaggatt gaaaatccgt
      181 gcaaattcga aaatccccac gattatgcga agcgcccacc gacaggacgc tgacgcacgg
      241 attacatttc tgtcagccaa ccgtgatcgt gccgatcatg ccggcctgga aatggccagg
      301 aatcaggcag gcgaactgga agtcccccgc gcggttgaag tgccagatca tttgcccgcg
      361 tccgcccggg ccgacatgtg ccatccacgg ctcgtcatgc tccatccctg ggaagcgccg
      421 catcagttcg gcgtgctcgg cgagcgtcgc gggcgtgccg accaccatct cgtgcatcac
      481 cgagccgcga ttctcgtggg tgaagcggat cgtctcgccc aggcgtatcg cgatacggtc
      541 ggggacgaaa cgcattgcat cggtcatcac gatggggatc gttctcgcaa cggatctctc
      601 atcgcccgcg attccccatt cctgctgttc cttgggggca tcggacgcag ccttgtgggg
      661 atgctcggcc ttgccatggg cgaacgcgct gcggtgagcg gcaagcacac cggccgcaag
      721 gctggtggcg aggaatcgac gacggcttct catggggatc tccatctgcc tggcaggtga
      781 atcagcacca cggccacgcc cacccctcca ccatcgtgga tgacctgtct ctgcatgggg
      841 aaatccgttt gctgatcttg tcctgagccg gacgcggggc gatcgacgag gtcgccgccc
      901 cgcatctcga ggctcagttg ggcatcgccg gcttgaccgc cggatgcatc ttcatcatgt
      961 tctgcatcat gcccatgtgc tcttgcatca tcttcatgtg ctcctgcacg agcctcgggt
     1021 ccatcatcat gccgcccatc cctccagccc cgggtccagc cccggcgccc ttcatcatcg
     1081 ggccggcgcc catcggtcct ccctgcatct ggccacccat catcgggcac acggcgggcg
     1141 tgccctgcga tgccgcctcc agggccttga tctgctcctc catcagggcc atgcgacgtt
     1201 cggggtcggt ctcggcacgg atcgacttca tgcgggcctg aagtgcgggg ccactcgctg
     1261 cgggcggcca gggctgcgcc tgctcggtcg tctgagcgag gaccggactg ccgtgcaggg
     1321 ctgcgaccag agcggcaacg ggcatgagcc cggcgagacg gaatgaacgc agtttcatga
     1381 tcgatctccc atcggttgcg gggttccgtc gcgatacgga acgatatgcc acgcggggca
     1441 tgttgcgccc gcccgcctgt cgtcggcctg acctggacat tacatttctg taattccgcc
     1501 tgctctcccg caccccggag gcagaatcgg tcaccgacag cacgaaccga gtaggagcga
     1561 aacaatgaag atcctgatcg tcgaagacga agtgaagacc ggcgactacc tgcgccaggg
     1621 cctgaccgag gccggattcg tcgtcgatct cgcgcgcgac ggcctcgatg gacttcacct
     1681 cgcgctcagc ggcgactacg acctcatcgt gctggacgtg atgctgccgt cgctggacgg
     1741 ctggggcatc ctgcagacac tgcgtcgcag cggtcgcgag aacccggtgt tgttcctgac
     1801 cgcccgtgac cagatcgagg accgcgttcg tggcctcgag cttggcgccg atgattacct
     1861 ggtcaagccc ttcgcattct ccgagttcct cgcccgggtc cgcaccctgc tgcgcagggg
     1921 gcggagccat gaaccggaca ccctgcgctc ggcgaatctc gaactggatc tgcttcgccg
     1981 ccgcgtgacg cgctgcgggc agcgcatcga cctgaccgcc aaggaattcg ccctgctcga
     2041 actgctgctg cggcgcaagg gcgaagtgtt gccgcgctcc ttgatcgcct cccaggtgtg
     2101 ggacatgaac ttcgacagcg acaccaatgt gatcgaggtc gctgtacgca ggctgagggt
     2161 gaaggtcgac gaccccttcg agcccaagct gatccgcacc gtgcgcggca tgggctacgt
     2221 gctcgaagcg atggagccga gctgacgatg atccgcatcc actcgctgac ggcgcgcctg
     2281 gcgacgctgt ttgccgtcct cgccgcgagc ctgctcgtgc tggtggcggt gctcttcggc
     2341 cgcatgctgg acgcgcattt ccaggagctg gacatgcacg agctgcaagg caaggtcacg
     2401 ctgatccgca acacgctgca ggatgaggct gcgagtggcg gacgcgcgga acgcatccag
     2461 gcgctcgagc gctccttcgt tggacacgaa agcgtggggg tgctgctacg cgacgtggac
     2521 gggcgcgtgc tgaacgtcat ccatcccgag cacttcaccg ctgcccagcg ggctggagaa
     2581 cgcccatcgg gcgtggtgac cgactggacg gtcgatgggc gcccccatcg aggggtcgag
     2641 gtcggcatcg cgctgccgcg cagcgggtcg aaggaccaaa cgatcgcagc gctggtcgca
     2701 ctcgatctgt cgcaccacgt ccatttcctc gcgtcggtgc gcaacgccac ctgggcgggc
     2761 gtggtcgcgg cagccatggc ggccgcgctc ttcggctggt ttgcggcgca ccggggcctc
     2821 gctcccctgc gccgggtcac cgaaaccgcc cgccggctgt cggcacggca actcgccgag
     2881 cgtctctcca tcgacgatgc accgctggag gtgcgcgacc acgccgaggc gttcaacggc
     2941 atgctcgccc ggctcgaggc cgcgttccag cgcctcggcg actattcggc cgacatcgcg
     3001 cacgaactgc gcacgccgat ctcgaacctg atgacccaga ctcaggtcgc gctatcgcgg
     3061 ccacggacga tcgacgagta ccaggacatc ctggcgtcga acctcgagga gtacgagcgc
     3121 atcgcgcgca tggtcagcga catgctgttt ctcgccaagg ccgaggagaa cacactggcg
     3181 catgccagcg aacccgtgga cctggcgctc gaggcggatg cactcatcga tttctacgag
     3241 gccctggccg aagagcgcca ggtccgcatc gttcgggagg ggcaggcgag cgtccagggt
     3301 gatcgactga tgctgcggcg cgccttgtcg aacctgattt cgaacgccct gcgccacacg
     3361 ccggacgagg ggcagatcac gatcaggatt gacgcggacg ccaccgacat ccgcctcgcc
     3421 gtctgtaatg tcggcgatcc gatccccggc gaccagatcg agcggatctt cgagcgcttc
     3481 catcgcggca gtgcccagcg cgaatttcgc ggtgaaggcg cagggctcgg gcttgccatc
     3541 acacgctcca tcgtccaggc ccacgggggt cagatcagtg cacagtcggc tgaaggtgcc
     3601 acatgcttca ccatcacctt gcccgggcac cgggcgccat cgtcttcttg atccagcgaa
     3661 ttcggcacgg gttcgggcgt tctgactggc tgcaacgagg ggcacctccc tgcggacgac
     3721 agccgacgac ttctctgcga gtccccggag gcagtcggac gggcttgatc gagtggggat
     3781 tcaggacctt tttccacgat gcgcgggtgg gcgagccgaa agcgccgcag atccgccgga
     3841 ccgtggcttc gccgcagcgc gcgcgctcga gcaggctttc gacgaccccg gcctgggtgt
     3901 catccgcctc gatggaacgg cgaggcgtct ggcgggttcg tcggcctcga ccctgacgag
     3961 tgcgttcggc tgcatggatc atccaaggac ttggaccatg cgccgggcat ggaccgcgca
     4021 tccccgctac cgagcaccca catgatgggc aaagcttcga cctgccgccg cgatcctccg
     4081 gcggcctcca gcatcccgca tggaacacga acatgaccat actgaccccc gcgcagcggc
     4141 gcgccacctg catccacgag gccggccatg cggtggtgtt cgcgctcggc ggcgtgtcgg
     4201 tctaccggct ggcggtggcg gccgcagggg ccgaggcctg gcgcaccgac atccgcaacg
     4261 gacgcatgtg caccgggctg tggggactgt gcgagaaggc ggacctggtg
//
LOCUS       DAOQNN010000274         4309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_12873_length_4309_cov_8.021862, whole genome shotgun sequence.
ACCESSION   DAOQNN010000274 DAOQNN010000000
VERSION     DAOQNN010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12873_length_4309_cov_8.021862"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKV97_12790"
     CDS             <1..287
                     /locus_tag="PKV97_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB06802.1"
                     /translation="AVLVAAADAGIGAPAVRFTVRDSGRGIAPELQERLFEPFFTTRA
                     EGTGLGLAIARGVARAHGGDITLRSAPGQGAEFTLVVPLAAAPIGAQENP"
     gene            284..1705
                     /locus_tag="PKV97_12795"
     CDS             284..1705
                     /locus_tag="PKV97_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB06803.1"
                     /translation="MIETLNILVVEDDAALRDAVCFTLEMAHHRVVGVDGGAAALAAI
                     EREHFNLVVTDLRMQPMDGLQVLHEVRARRPQTPVLLMTAYGDVDKAVAAMRGGACDF
                     LMKPFEPEVLLESVRRYAAVQPAGEDTIAEDPHTRGLLALAARVAESDATVMLSGESG
                     TGKEVFARYIHQHSPRAKGPFIAINCAAIPENLLEATLFGYEKGAFTGAQAAQPGKFE
                     QAQGGTILLDEISEMPLGLQAKLLRVLQEREVERVGGKKPVALDIRVLATSNRDMLGE
                     VAAGHFREDLYYRLNVFPIAIPALRERPGDIVPLARHFLARHAARQQRQVRFSPAAEA
                     LLPQYAWPGNVRELENIMHRVLILTQGDEVSAETIRLCLPQWKPEPANSAGGAAGLAA
                     SPGRQGGVAAVQSGSGQAAGAGTAGPAPERPTNMKDLEREHILATLREFGGSRKKTVE
                     KLGISERTLRYKLQQYREEGHDV"
     gene            2024..2347
                     /gene="fliE"
                     /locus_tag="PKV97_12800"
     CDS             2024..2347
                     /gene="fliE"
                     /locus_tag="PKV97_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNB06804.1"
                     /translation="MDTRGIDQMLSELRSVSQAAQGKAAPAADAPAGGVNFADVLNTA
                     LKDVSAAQGEARAMAQNFSAGDPNVNLQDVMVNLQKASMSFQQMVQVRNRLVTAYQDI
                     MNMPV"
     gene            complement(2525..3316)
                     /locus_tag="PKV97_12805"
     CDS             complement(2525..3316)
                     /locus_tag="PKV97_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNB06805.1"
                     /translation="MAEPSTPSPASPAPLDGGRGDELEKFTLRGARQILQLLQDLITH
                     RGLITAHTGGGHSFMTAVLKVDEERGRVVLDPSPDPQANRRALAAPRLTCVTQLDGIR
                     IQFPLVGLGEGQDKGRPALFAPLPAEMLRLQRREFYRLQVPLAHELSCLLKAEDLARK
                     PVEVSARVIDIGAGGVAVVVPTGAAEFVIGGTLPACRLALPDGEPIELDLEVRNLNRQ
                     TQRNGTEQLRVGLRFAALPRAADTRIQRYIFKTERALNAKARGGL"
     gene            complement(3370..4149)
                     /locus_tag="PKV97_12810"
     CDS             complement(3370..4149)
                     /locus_tag="PKV97_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNB06806.1"
                     /translation="MTIDQEDTRIELIHADDEAKYVLRDAREILAIVRNLLGARALVS
                     ARLAPGNESVLSTLVAVAEDGTSLLLDGSADPLQNQRMERADALDCVTQLDKVRIQFL
                     LRGQRLVEDGGSPGFRAPLPEALLRLQRREFYRLQTPISEGVSCTLALPRVDGSRREA
                     ELRILDISGGGVAIAVPPVGVHFEPGTEFPDSLLRLPDTAPISARLVVRNLFRITRRG
                     GAEVLRAGCEFADLPRGAEDAIQRYILRAERTRNARERGRL"
BASE COUNT          619 a         1511 c         1553 g          626 t
ORIGIN      
        1 aggcggtgct ggtggccgcc gccgacgccg gcatcggcgc gcccgcggtg cgcttcacgg
       61 tgcgcgacag cggccggggc atcgcccccg agctgcagga gcgcctcttc gaacccttct
      121 tcaccacccg cgccgaaggc accgggctgg gcctggcgat cgcccgcggc gtcgcgcgcg
      181 cccacggcgg cgacatcacc ctgcgctccg cgcccgggca gggcgcggaa ttcaccctcg
      241 tcgtgccgct ggcggcggcg cccattggcg cacaagagaa cccatgatcg agaccctcaa
      301 catcctcgtc gtcgaggacg acgccgcgct gcgcgacgcg gtctgtttca cgctggagat
      361 ggcgcaccac cgcgtcgtcg gcgtcgatgg cggcgcggcg gcgctcgcgg cgatcgagcg
      421 cgagcacttc aacctggtcg tcaccgacct gcgcatgcag cccatggacg gcctgcaggt
      481 gctgcacgag gtgcgcgcgc gccggccgca gacgccggtg ctgctgatga ccgcctatgg
      541 cgacgtcgac aaggcggtgg cggcgatgcg cggcggcgcc tgcgacttcc tgatgaagcc
      601 cttcgagccc gaggtgctgc tcgagagcgt gcgccgctac gccgcggtgc agcccgcggg
      661 cgaggacacc atcgccgagg acccccacac ccgcggcctg ctcgcgctcg ccgcgcgcgt
      721 ggccgagtcg gatgcgacgg tgatgctctc gggcgagtcc ggcaccggca aggaggtctt
      781 cgcgcgttac atccaccagc actcgccgcg cgccaagggc cccttcatcg cgatcaactg
      841 tgcggccatc cccgagaacc tgctcgaggc cacgctcttc ggctacgaga agggcgcctt
      901 caccggtgcg caggcggcgc agccgggcaa gttcgagcag gcccagggcg gcaccatcct
      961 gctcgacgag atctccgaga tgccgctcgg cctgcaggcc aagctgctgc gcgtgctgca
     1021 ggagcgcgag gtcgagcgcg tcggcggcaa gaagccggtg gccctcgaca tccgcgtgct
     1081 cgccaccagc aaccgcgaca tgctcggcga ggtcgccgcc gggcacttcc gcgaggatct
     1141 gtattaccgc ctcaacgtgt tcccgatcgc catccccgcg ctgcgcgagc gcccgggcga
     1201 catcgtgccg ctggcgcgcc acttcctcgc ccgccacgcc gcccgccagc agcgccaggt
     1261 gcgcttctcg cccgccgccg aggcgctgct gccgcagtac gcctggccgg gcaacgtgcg
     1321 cgagctggaa aacatcatgc accgcgtgct gatcctgacc cagggcgacg aggtgagcgc
     1381 cgagaccatc cgcctgtgct tgccacaatg gaagccggag ccggcaaatt ctgccggcgg
     1441 ggcggcaggg cttgccgctt ctcccgggag gcaggggggt gtcgcggccg tgcagtcggg
     1501 tagcgggcag gccgccgggg ccggcacggc cggtccggct cccgagcgcc cgaccaacat
     1561 gaaagacctc gagcgcgagc acatcctcgc cacgctgcgc gagttcggcg gctcgcgcaa
     1621 gaagaccgtg gaaaagctcg gcatctccga gcgcaccctg cgctacaagc tgcagcaata
     1681 ccgcgaggag ggccacgacg tgtgagacct ggcgccctgt gggagcgacg cgagtcgcga
     1741 tcccggcccc gcagcccttt ccggccgccc atcggccacc ggattgcacg atcgcccgct
     1801 cgcgacttgc gtcgctccta cggcaagtca accttcttcc gaaggcgcct gtgggagcga
     1861 cgcaagtcgc gatcgggcgc atggaatcag tgccggcgcc gtcgtcgcga cttgcgtcgc
     1921 tcctacgaaa accgcaacga ccgcgcgtcc tcggcggctc tggcacgcga tttgctttgc
     1981 tacactgacg tcaccgacca ccctgaagcc gggggattac gctatggaca cccgtggaat
     2041 cgaccagatg ctgagcgaac tgcgctcggt gtcgcaggcc gcgcaaggca aggccgcgcc
     2101 cgccgcggac gcgcccgcgg gaggcgtcaa cttcgccgac gtgctcaaca ccgccttgaa
     2161 agacgtcagc gccgcccagg gcgaggcgcg cgcgatggcg caaaacttct ccgccggcga
     2221 ccccaacgtc aatctgcagg acgtcatggt caacctgcaa aaggccagca tgtccttcca
     2281 gcagatggtc caggtccgca accgcctggt cacggcgtat caggacatca tgaacatgcc
     2341 ggtctgaccg gcttcgccgc ggttctcccg taggagcgac gcaagtcgcg accggggcgt
     2401 tgtcgcgcat ttcgagcgcc ggaatcgcga attgcgtcgc tcctacgaaa acccgcggcg
     2461 tctcgggtgg gatgcaggtg acgcgagtcg cgaccggggc gctgcggaaa ggcttggcgt
     2521 catcctacag gcctccgcgt gccttggcgt tgagcgcgcg ttccgtcttg aagatgtagc
     2581 gctggatgcg ggtgtccgca gcgcgcggca gggcggcgaa gcgcaggccc acccgcagtt
     2641 gttcggtgcc gttgcgctgc gtctggcggt tgaggtttct cacctcgagg tcgagttcga
     2701 tcggctcgcc gtccggcagc gcgaggcggc aggccggcag tgtgccgccg atgacgaatt
     2761 ccgccgcccc cgtgggcacc accaccgcca cccctccggc accgatgtcg atcacgcgtg
     2821 cgctcacctc caccggcttg cgggcgaggt cctccgcttt cagcaggcag ctcagctcgt
     2881 gcgcgagcgg tacctgcagg cggtagaact cgcgccgctg caggcgcagc atctcggcgg
     2941 gcaggggcgc gaagagggcg gggcggccct tgtcctggcc ttcgccgagg ccgacgagcg
     3001 ggaactggat gcggatgccg tcgagctggg tcacgcaggt caggcgcggc gcggcgagcg
     3061 cacggcggtt ggcctggggg tcggggctgg ggtcgagcac cacgcgcccg cgctcttcgt
     3121 ccaccttcag caccgcggtc atgaaggagt ggccgccgcc ggtgtgggcg gtgatcaagc
     3181 cgcggtgggt gatgagatcc tgcagcagct gcaggatctg gcgcgcgccg cgcagggtga
     3241 atttctcgag ctcgtcgccg cgtccgccgt cgagcggtgc cgggctggcg gggcttgggg
     3301 tgctgggttc ggccatgcgg ggctatcgtg tcttgggcag ggtcgggagg cgggtggggt
     3361 gccgcgggtt cacaagcggc cgcgctcgcg cgcgttgcgg gtgcgctcgg cgcgcaggat
     3421 gtagcgctgg atcgcgtcct cggcgccgcg cggcaggtcg gcgaactcgc agccggcacg
     3481 cagcacctcg gcgccaccgc gccgggtgat gcggaagagg ttgcgcacca ccaggcgcgc
     3541 ggaaatcggt gcggtgtcgg gcaggcgcag caggctgtcg gggaactcgg ttccgggctc
     3601 gaagtgcacg cccaccggcg gcaccgcgat cgccacgccg ccgccgctga tgtcgaggat
     3661 gcgcagctcg gcctcgcgcc ggctgccgtc gacacggggg agggccagcg tgcagctcac
     3721 cccctccgag atcggcgtct gcaggcggta gaactcgcgc cgctgcaggc gcagcagggc
     3781 ttccggcagc ggcgcgcgga agcccgggct gccgccgtcc tcgacgaggc gctggccgcg
     3841 cagcaggaac tggatacgca ccttgtcgag ctgggtcacg cagtcgagcg cgtcggcgcg
     3901 ctccatgcgc tggttctgca gcgggtcggc gctgccgtcg agcagcaggc tggtgccgtc
     3961 ttccgcgacc gcgaccaggg tggacagcac ggattcgttg cccggcgcca gccgtgcgct
     4021 cacgagcgcc cgcgcaccga gcaggttgcg cacgatggcg agaatctcgc gcgcgtcgcg
     4081 cagcacgtac ttcgcctcgt cgtcggcgtg gatgagttcg atgcgggtgt cttcttggtc
     4141 gatcgtcatg ccttggggct cgtcgcttga ggcagcgcac ggctgctgca caaactcgtt
     4201 ttcttatatc acgagacacg ccccgggggc gtggtagcgc gccccgcgag gccgcggcgt
     4261 ccgggaggcc ccgcaggtgc gatgcaagtc gcgatcatcg gggcgtgga
//
LOCUS       DAOQNN010000275         4297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_12937_length_4297_cov_9.477133, whole genome shotgun sequence.
ACCESSION   DAOQNN010000275 DAOQNN010000000
VERSION     DAOQNN010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12937_length_4297_cov_9.477133"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="PKV97_12815"
     CDS             <1..1345
                     /locus_tag="PKV97_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06807.1"
                     /translation="PPAQAKAAPKPRARTLGTSGDESLVTLASSLYPDDPAAQARFIE
                     ATAAANPKLFADAAALTRVLPAGTALRLPDLRPAAPQPEAAPAPVVAQGPDRLVVDKT
                     VATRSTGGTTAAHGDAPPRNHAERERALAAAIDRSIIAEMELLARIKELEEIQAGLKE
                     RLLRMQAVPAVATAPAVAVPASPPPRTEPVTPAETRPALAAENAWGRDAYLFGGLGFA
                     ALALVALLRRSRRPTPAAPSSRPTDTATATTLGSAESRKGRARAEETGFGFGVGTVDT
                     RLEWPAGTATETIQEHKSAVELADIMMSFGRVRGAAETLAEFIRGNPREAVSPWLKLL
                     EVYRAAGLRDEFNAIAGELNKTFNVTTVNWQSYDALRASKMSLEDLPHICEALQKTWR
                     TAACQRHLQQLLRDNRNGTRAGFPFSVIDEILTLTAILEEDLGPCPPAADEPPRR"
     gene            complement(1342..2091)
                     /locus_tag="PKV97_12820"
     CDS             complement(1342..2091)
                     /locus_tag="PKV97_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNB06808.1"
                     /translation="MQLTELGGHLDTLLDASRLKDYCPNGLQVEGRAEVRRVLCGVTA
                     SQALLDLAATGAYDAVFVHHGLFWKGDDGRVTGFRRRRLHTLLANDISLFAYHLPLDV
                     HPELGNNAQLARLMGWQGTARFADQELGWIGRPAREGERADEIARALARRLGREPLLV
                     GDGERPVRRIAWCTGGAQGFFEQAILAGAELYVSGEISEPTVHLARESGVPYIAAGHH
                     ATERYGARAMAHYLNEVLGLEASFVDLDNPV"
     gene            2187..3338
                     /locus_tag="PKV97_12825"
     CDS             2187..3338
                     /locus_tag="PKV97_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNB06809.1"
                     /translation="MRRLWLIFAQAVTVSVAVLFVLNTLKPEWLRKATPAAVVAILEA
                     PAGSERAPAAGSYAPAAQRSMPAVVHIFTSKGGRGQRHPLLDDPLFRHFFGERPDSAP
                     SESGLGSGVIVSPDGYVLTNNHVIETADAIEVALNDGRKFAARLVGRDPETDLAVLRI
                     DGAEALPAITFPAADSLAVGDVVLAIGNPFGVGQTVTMGIVSALGRSQLGINTFENYI
                     QTDAAINPGNSGGALVDSAGSLVGINTAIYSRSGGSLGIGFAIPVSIARDVLEQIVAT
                     GEVVRGWVGVEIQDLTPELAASFGYRDAGGALIAGVLRGSPADRAGVRPGDVLVALDG
                     ESVRDPRAMLDMVAALSPGKRAVFRVRRGAEALDLEVEVGRRPIPPAAR"
     gene            complement(3366..4163)
                     /gene="tatC"
                     /locus_tag="PKV97_12830"
     CDS             complement(3366..4163)
                     /gene="tatC"
                     /locus_tag="PKV97_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNB06810.1"
                     /translation="MSEQQETFIAHLIELRDRLIRALLAIVIVFVCLMPWAGAIYDIL
                     AKPMMDTLPAGTNMIATGVVTPFFVPVKVTMMVAFVFALPWVLYQAWAFIAPGLYAHE
                     KRLALPLVLGSTLLFLLGMAFCYFFVFGTVFKFIAEFAPKSIVPAPDIEQYLSFVMSM
                     FIAFGITFEVPVAVILMVKAGIVDVAKLRESRPYVIVGAFVVAAIVTPPDVVSQFMLA
                     VPMCLLYELGIVLAGMLSRPSPQQEAAGETVQQLAGPAAADGEGEPR"
BASE COUNT          679 a         1585 c         1460 g          573 t
ORIGIN      
        1 accgcccgcg caggcgaagg cggcgccgaa accacgcgcg cgcaccctcg gcacgagcgg
       61 cgacgaatcg ctcgtcaccc tggcgtcgtc gctgtacccg gacgaccccg ccgcgcaggc
      121 gcgcttcatc gaggcgaccg ccgcggcaaa cccgaagctg ttcgccgacg ccgccgcact
      181 cacccgcgtc ctgcccgcag gcacggcgct gcgcctgccc gacctgcgcc ccgccgcgcc
      241 gcagcccgaa gcggcaccgg caccggttgt cgcccaggga cccgaccggc tcgtcgtgga
      301 caagaccgtc gccacgcgga gcacgggcgg gaccacggcg gcgcatggcg acgccccccc
      361 gcgcaatcac gcggagcgcg agcgcgcgct tgccgcggcg atcgaccgca gcatcatcgc
      421 cgagatggag ctgctcgccc gcatcaagga gctcgaggaa atccaggccg ggctgaagga
      481 gcgcctgctc aggatgcagg ccgtccccgc cgtcgcaacc gccccggccg tcgcagtccc
      541 cgcctcgcct ccgccgcgga ccgagccggt cacccccgcc gaaacgcgcc ctgcgctcgc
      601 ggccgagaac gcctggggcc gcgacgccta cctgttcggc ggactcgggt ttgccgcctt
      661 ggctctcgtc gcgctgttgc gccgctcgcg gcgtccgacg ccggcagcgc cgtccagtcg
      721 gccgacggac accgccaccg cgacgacgct cggcagcgcg gagtcccgca agggccgcgc
      781 gcgggccgaa gaaaccggct tcggtttcgg cgtcggcacc gtcgacactc gcctcgagtg
      841 gccggcgggg acggcaaccg agaccatcca ggagcacaag tccgcggtcg agctcgccga
      901 catcatgatg agcttcggcc gcgtccgcgg cgcagccgag acgctcgccg agttcatccg
      961 cggcaatccg cgcgaagcgg tctcgccctg gctcaagctg ctcgaggtct accgggcagc
     1021 gggcctgcgc gacgagttca acgcgatcgc cggggagctc aacaagacct tcaacgtcac
     1081 cacggtcaac tggcagagct acgacgcgct gcgggcgtcg aagatgagcc tggaggacct
     1141 gccgcacatc tgcgaggccc tgcagaagac atggcgcacc gccgcctgcc agcgccacct
     1201 gcagcagctg ctgcgcgaca atcgcaacgg cacccgcgcc ggcttcccgt tctcggtgat
     1261 cgacgagatc ctcaccctca ccgccatcct cgaggaagac ttgggcccct gcccgccggc
     1321 cgcggacgag cccccccggc gctagaccgg gttatcgagg tcgacgaagc tcgcctccag
     1381 cccgagtacc tcgttcaggt agtgcgccat ggcgcgtgcg ccgtagcgct cggtggcgtg
     1441 gtggccggcg gcgatgtagg gcacacccga ctcgcgcgcc agatgcaccg tgggctcgga
     1501 gatctcgccc gacacgtaga gctcggcccc ggccaggatc gcctgctcga aaaagccctg
     1561 cgcaccgccg gtgcaccagg cgatccggcg caccggacgc tcgccgtcac cgaccagcag
     1621 cggctcgcgc ccgagccgcc gggcaagcgc gcgcgcgatc tcgtccgcac gctcgccctc
     1681 ccgcgccggc cggccgatcc agcccagctc ctggtcggcg aagcgcgccg tcccctgcca
     1741 gcccatcagg cgcgcgagtt gcgcgttgtt gccgagctcc ggatgcacgt cgagcggcag
     1801 atggtaggcg aacaggctga tgtcgttggc gagcagggta tgcaggcgcc gacgccggaa
     1861 gccggtcacg cgaccgtcgt cgcccttcca gaacaggcca tggtgaacga agacggcgtc
     1921 atacgccccc gttgctgcaa ggtcgagcag ggcctggctg gcagtcaccc cgcacagcac
     1981 gcgccggacc tccgcgcgtc cttccacttg caggccgttt gggcaataat ccttcagccg
     2041 tgaagcgtcg agcagggtgt cgagatgacc cccgagttcg gtgagctgca tgcgtcctcc
     2101 ttcatccttg cttcgcgcgt ggctcctcga ccggagaacg ctccggccgg ccgggcgcga
     2161 ccccttcttc tttgaagccc gacattatgc gccgcttgtg gctcatcttc gcgcaggcgg
     2221 tgaccgtcag cgtcgccgtg ctgttcgtgc tcaacaccct caagccggaa tggctgcgca
     2281 aggccacgcc cgcggcggtg gtggcgatcc tcgaggcacc tgcgggcagc gagcgtgcgc
     2341 cggcggcagg ctcctacgcc ccggctgcac agcgttcgat gccggcggta gtgcacatct
     2401 tcaccagcaa gggcggtcgc ggccagcgcc acccgctgct cgacgacccg ctgttccgcc
     2461 acttcttcgg ggagcgcccg gacagcgcac cgagcgaatc cggcctcggc tcgggcgtca
     2521 tcgtcagccc cgacggctac gtactcacca acaaccacgt catcgagacc gccgacgcga
     2581 tcgaggtcgc gctcaacgac ggccgcaagt tcgccgcccg cctggtcggg cgcgaccccg
     2641 agaccgacct cgcggtgctg cgcatcgacg gcgccgaggc gcttccggcg atcaccttcc
     2701 cggccgccga cagcctcgcg gtgggcgacg tggtgctggc gatcggcaac cccttcggcg
     2761 tcggccagac ggtcaccatg ggcatcgtct cggcgctcgg tcgcagccag ctcggcatca
     2821 acaccttcga gaactacatc cagaccgacg ccgcgatcaa cccgggcaac tcgggtggcg
     2881 cgctggtcga cagcgcgggc agcctggtcg gcatcaacac ggcgatctac tcgcgctcgg
     2941 gcggttcgct cggcatcggc ttcgcaatcc cggtctcgat cgcgcgcgac gtgctcgagc
     3001 agatcgtcgc caccggcgag gtcgtgcgcg gctgggtggg cgtggagatc caggacctca
     3061 cacccgaact ggccgcctcg ttcggctatc gcgacgccgg cggcgcgctc atcgccggcg
     3121 tactgcgcgg cagcccggca gatcgggcgg gcgtgcgtcc gggcgacgtt ctggtcgcgc
     3181 tcgacggcga gagcgtgcgc gacccgcgcg cgatgctcga catggtcgcg gcgctctcac
     3241 ccggcaagcg tgcggtgttc agggtgcggc gcggcgccga ggcgctcgac ctcgaggtgg
     3301 aagtgggccg ccgcccgatc ccgcccgccg cgcgctgagg caccgaccga cgcggcccgc
     3361 ggcactcagc ggggctcgcc ctcgccatcc gcagcagccg gcccggcgag ctgctgtacc
     3421 gtctcgccgg cggcctcctg ctgaggggaa ggcctggaca gcatgcccgc gagcacgatg
     3481 ccaagctcat agagcaggca catcggcacg gccagcatga actgcgagac cacatccggc
     3541 ggggtgacga tggcggcgac cacaaaggcg ccgacgatca cgtaagggcg cgactcgcgc
     3601 agcttggcga catcgacgat gccggccttg accatcagga tcaccgccac cggcacctcg
     3661 aaggtgatgc cgaaggcgat gaacatcgac atcacgaagg acaggtactg ctcgatgtcc
     3721 ggcgccggga cgatgctctt gggagcgaat tcggcgatga acttgaacac cgtgccgaac
     3781 acgaagaaat agcagaaggc catgccgagc aggaaaagca gcgtgctgcc gagcaccagc
     3841 ggcagggcga ggcgcttctc gtgcgcgtac agcccgggcg cgatgaaggc ccaggcctgg
     3901 tagagcaccc agggcagcgc gaacacgaag gcgaccatca tcgtcacctt gaccgggacg
     3961 aagaagggcg tcaccacgcc ggtggcgatc atgttggtac ccgcaggcag cgtgtccatc
     4021 atcggcttgg cgaggatgtc gtagatcgcg cccgcccacg gcatcaggca gacgaagacg
     4081 atcacgatcg cgagcagcgc gcggatcagg cggtcgcgca gctcgatcag gtgggcgatg
     4141 aaggtttcct gttgttcgct catgccttga ccttgtccgc tccaggcgcg gtgcggggcg
     4201 tgctatccag cccgagctcg agttgcgggg cgggacgggc gggcgtcgca tccgctccgg
     4261 ccggcgcggg cgctgccgcc ggattcgcga cgggcgt
//
LOCUS       DAOQNN010000276         4282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13021_length_4282_cov_8.683700, whole genome shotgun sequence.
ACCESSION   DAOQNN010000276 DAOQNN010000000
VERSION     DAOQNN010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13021_length_4282_cov_8.683700"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKV97_12835"
     CDS             <1..311
                     /locus_tag="PKV97_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB06811.1"
                     /translation="VPVAEVRYLRAELKYVTARTAVREYVLDESLVQIEQEFPRSFLR
                     LHRNCLVALAAVAGVERAAERDGQKEAEPRWEVLLRDLPERLPVSRRQWPAVRQALGL
                     "
     gene            complement(318..3080)
                     /gene="ppc"
                     /locus_tag="PKV97_12840"
     CDS             complement(318..3080)
                     /gene="ppc"
                     /locus_tag="PKV97_12840"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNB06812.1"
                     /translation="MNQDKDAPLRDDIRLLGRLLGDTVRDQQGEAAFGLIERIRQNSV
                     RFRRDDDIAARRELEDILDALSREQTIQVVRAFSYFSHLANIAEDQHHIRRSRAHLIA
                     GSAPREGSLAHALERALDSGAADPAALAGFFDGALVSPVLTAHPTEVQRKSILNCETV
                     IARLLDARDRMQLTPEEAEANDEALRRAVLTLWQTRMLRTAKLSVIDEVNNGLSYFET
                     TFLRELPRLYASLEDRLAAADRALGTPELAAFVQVGSWIGGDRDGNPFVTAEVLERAL
                     AMQCAVALGHYLDELHILGSQLSLGIGLVSASDALLALAEASPDHSPHRSDEPYRRAI
                     SGIYARLAATYRSLLGHDPLRHAVAAAQPYPSAAALAEDLEVLHRSLAANGSGALTRG
                     RLRHLRRAVKVFGFHLAPLDLRQNSDVHERTVAELLERACPGTAYASLDEDARCALLL
                     EELATARPLASPHVRYTDETEGELAIFRAARQAHLRYGTAAIRNCIISKTDDVSDLLE
                     LAVLLKEAGLLRPLENALDVNIVPLFETIGDLENAAGVMARIFSIPAYRGLLEARDHT
                     QEVMLGYSDSNKDGGFLTSGWALYKAEGELVETFARHGVRLRLFHGRGGSVGRGGGPS
                     YQAILAQPEGAVQGQIRLTEQGEVIGAKYGNPEVGRRNLEVLVAATLESSLRPASASP
                     TPPAFLDAMQALSDAAFAAYRGLVYETEGFERYFWESTVISEIAALNIGSRPASRKKS
                     TAIEDLRAIPWVFSWSQCRVMLPGWYGFGSAVKDFLTANPDDGLALLQRMNMEWPFFQ
                     TLLSNMDMVLSKTDLAIASRYAELVKDAALRDTIFGRIRAEHQATVGAVLRITGQSEL
                     LDANPLLKRSIRNRFPYLDPLNHVQVELLRRHREAAEAGGIDERIRLGIHLSINGIAA
                     GLRNSG"
     gene            3338..4270
                     /gene="hemC"
                     /locus_tag="PKV97_12845"
     CDS             3338..4270
                     /gene="hemC"
                     /locus_tag="PKV97_12845"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNB06813.1"
                     /translation="MSQSSPERIVIATRESRLALWQAEHIKARLEALHPGCRVDLLGM
                     TTRGDQILDRPLAKVGGKGLFVKELETALLDGRADIAVHSMKDVPMVMEPEFALPCIT
                     AREVPLDAFVSSRYESLADMPPGAVVGTSSLRRESQIHAQYPFLGVTSLRGNLDTRLR
                     KLDEGQYDAIILAAAGLTRLGLGERIRSVMPAEVSLPAAGQGALGIECLSARADVIGW
                     LQPLVDADTTACVLAERAVARALAGSCEVPLGAYAVMREGRLWLRGFVARPDGSTIVR
                     AEREGAPAEAEALGRALADDLRAQGAEAILAELG"
BASE COUNT          574 a         1417 c         1627 g          664 t
ORIGIN      
        1 tggtgcctgt ggccgaggtg cgctatctgc gtgccgagct caagtacgtc accgcgcgca
       61 ccgcggtgcg cgagtatgtg ctcgacgagt cgctggtgca gatcgagcag gagtttccgc
      121 gcagtttcct gcgcctgcac cgcaactgcc tggtcgcgct cgccgcagtg gcgggtgtgg
      181 agcgggcagc ggagcgggat ggacagaagg aggcggagcc gcgctgggag gtgctgttgc
      241 gcgatctgcc cgagcgcctg ccggtgagcc ggcggcagtg gccggcggtg cggcaggcgc
      301 tcggcctgta agacggctca gccgctgttg cgcaagcccg cggcgatgcc gttgatggag
      361 aggtggatgc cgagccggat gcgctcgtcg atgccgcccg cctcggcggc ctcgcggtgg
      421 cggcgcagca gctcgacctg gacgtggttg agcgggtcga ggtaggggaa gcggttgcgg
      481 atcgagcgct tgagcagcgg gttggcgtcg agcagctcgc tctggccggt gatccgcagc
      541 accgccccga ccgtggcctg gtgctcggcg cggatgcgcc cgaagatcgt gtcgcgcagc
      601 gccgcgtcct tgaccagctc ggcgtagcgg ctggcgatcg cgaggtcggt cttggacagc
      661 accatgtcca tgttcgacag cagggtctgg aagaagggcc attccatgtt catgcgctgc
      721 aagagcgcca ggccgtcgtc cgggttggcg gtgaggaagt ccttcaccgc gctgccgaag
      781 ccgtaccagc ccggcagcat cacccggcac tgcgaccagc tgaacaccca ggggatcgcg
      841 cgcaggtcct cgatcgcggt gctcttcttg cgcgaggccg ggcggctgcc gatgttgagc
      901 gctgcgatct cgctgatcac ggtcgactcc cagaagtagc gctcgaaacc ctcggtctcg
      961 tagaccaggc cgcggtaggc cgcgaaggcc gcgtccgaga gcgcctgcat ggcgtcgagg
     1021 aaggcgggcg gcgtcgggct ggcgctggcc gggcgcaggc tggactcgag cgtggcggcg
     1081 accagcacct cgaggttgcg tcggccgact tccggattgc catatttggc gccgatcacc
     1141 tcgccctgtt cggtgaggcg gatctgcccc tgcaccgcgc cctcgggctg ggcgaggatg
     1201 gcctggtagc tcgggccgcc gccgcggccc accgagcccc cgcggccgtg gaagaggcgc
     1261 aggcgcacgc cgtggcgcgc gaaggtttcg accagctcgc cctcggcctt gtagagcgcc
     1321 cagcccgagg tcaggaagcc gccgtccttg ttgctgtcgg agtagccgag catgacctcc
     1381 tgggtgtggt cgcgcgcttc gagcagtccg cggtaggcgg ggatcgagaa gatgcgcgcc
     1441 atcacgccgg cggcgttctc gaggtcgccg atggtctcga agagcggcac gatgttcacg
     1501 tcgagcgcgt tctcgagcgg gcgcaggagg cccgcctcct tcagcagtac cgcgagttcg
     1561 agcaggtcgg agacgtcgtc ggtcttggag atgatgcagt tgcggatcgc cgccgtgccg
     1621 tagcgcaggt gggcctggcg ggcggcgcgg aagatcgcca gctcgccctc ggtctcgtcc
     1681 gtgtagcgga cgtgcggcga ggcgagcggg cgcgcggtgg cgagttcttc gagcagcagc
     1741 gcacagcgcg cgtcctcgtc gaggctggca taggcggtgc cggggcaggc gcgttcgagc
     1801 agctcggcga ccgtgcgttc gtgcacatcg gagttctggc gcaggtcgag cggggcgaga
     1861 tggaagccga acaccttcac cgcgcggcgc aggtggcgca gtcgcccgcg cgtgagcgcg
     1921 cccgagccgt tggcggcgag cgagcggtgc agcacctcga ggtcctcggc gagcgcggcc
     1981 gcgctcgggt agggctgggc ggcggcgacc gcgtggcgta gcgggtcgtg gccaagcagg
     2041 ctgcggtagg tggcggccag gcgcgcgtag atgcccgaga tcgcacgccg gtagggctcg
     2101 tcgctgcgat gcggcgagtg gtcgggcgag gcctcggcaa gcgcgagcag ggcatcggag
     2161 gccgagacca ggccgatgcc gagcgagagc tgcgagccca ggatgtgcaa ctcgtccaga
     2221 taatgcccga gcgcgaccgc gcactgcatg gcgagcgcgc gctcgagcac ctcggcggtg
     2281 acgaaggggt tgccgtcgcg gtcgccgccg atccagctcc ccacctgcac gaaggcggcg
     2341 agctcgggcg tgcccagcgc ccggtcggcg gcggcgaggc ggtcctccag gctggcgtag
     2401 agccgtggca gttcgcgcag gaaggtggtc tcgaagtagg acaggccgtt gttgacctcg
     2461 tcgatcaccg acagcttggc ggtgcgcagc atgcgcgtct gccacagcgt cagcaccgcg
     2521 cggcgcagcg cctcgtcgtt ggcctcggcc tcctcggggg tgagctgcat gcggtcgcgc
     2581 gcatccagga gccgcgcgat cacggtctcg cagttgagaa tgctcttcct ctgcacctcg
     2641 gtggggtggg cggtgagcac cggcgacacc agcgcgccgt cgaagaaacc ggccagcgcc
     2701 gcggggtcgg ccgcgccgct gtcgagcgcg cgctccagcg cgtgcgccag gctgccctcg
     2761 cgcggtgccg agccggcgat caggtgggcg cgcgagcgcc ggatgtggtg ctggtcctcg
     2821 gcgatgttgg ccaggtggga gaaatagctg aaggcgcgca ccacctggat ggtctgctcg
     2881 cgtgacaagg cgtcgaggat gtcctccagc tcgcggcgcg cggcgatgtc gtcgtcgcgg
     2941 cggaagcgca ccgagttctg gcggatgcgt tcgatgaggc cgaatgcagc ctcgccctgc
     3001 tggtcgcgca cggtgtcgcc gagcaggcgg ccgagcaggc ggatgtcgtc gcggaggggg
     3061 gcgtccttgt cctggttcat tctttcgttt cggaattcga ttggagggga gagcggagct
     3121 taccgcaagc gggtcgcttt cctgcccgtt tcaggtcgat ccccggtgct gccgtctttc
     3181 gcgtcggctg cccggtggga ggcggcgcgg ccggcccgcg tgctagaatg cgcccagtcg
     3241 tgcggacgca ttcagcattc tgtcatgttg cgatgcatca tcctggcgcc cgtcgcctcc
     3301 gcacctgtgc ccccgttttt ccaggtgaat cccgatcgtg agtcaaagca gccccgagcg
     3361 catcgtcatc gcaacccgtg agagccgtct cgccctgtgg caggccgagc acatcaaggc
     3421 ccgcctcgag gcgctccacc ccggctgccg ggtcgatctg ctgggcatga ccacgcgtgg
     3481 cgaccagatc ctcgatcgtc cgctcgcgaa ggtcggcggc aaggggctgt tcgtcaagga
     3541 gctcgagacc gcgctgctcg acggccgcgc cgacatcgcc gtgcattcga tgaaggacgt
     3601 gccgatggtg atggagccgg agttcgcgct gccctgcatc acggcgcgcg aggtcccgct
     3661 cgacgccttc gtctccagcc gctacgagag cctcgccgac atgcccccgg gtgccgtggt
     3721 cggcacctcc tcgctgcgcc gcgagtcgca gatccacgcg cagtatccct tcctcggcgt
     3781 caccagcttg cgtggcaacc tcgatacccg cctgcgcaag ctcgacgagg gtcagtacga
     3841 cgccatcatc ctcgccgccg ccggcctcac gcggctcggc ctgggcgagc gcatccgctc
     3901 ggtgatgccg gccgaggtct cgttgcccgc ggccgggcag ggcgcgctcg gcatcgaatg
     3961 cctgtccgcg cgcgcggacg tgatcggctg gctgcagccg ctcgtcgatg ccgacaccac
     4021 cgcgtgcgtc ctcgccgagc gcgcggtcgc acgcgcgctc gccggcagct gcgaggtgcc
     4081 gctgggcgcc tacgcggtga tgcgcgaggg gcggctgtgg ttgcgcggct tcgtcgcgcg
     4141 ccccgacggc agcaccatcg tgcgcgccga acgcgagggc gcgccggccg aggccgaggc
     4201 gctcgggcgc gcgctcgccg acgacctgcg ggcgcagggc gcggaagcga tcctcgccga
     4261 gctcggctga gcggacggca ag
//
LOCUS       DAOQNN010000277         4279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13037_length_4279_cov_10.552794, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000277 DAOQNN010000000
VERSION     DAOQNN010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13037_length_4279_cov_10.552794"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKV97_12850"
     CDS             complement(<1..353)
                     /locus_tag="PKV97_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNB06814.1"
                     /translation="MSVTDTQISKSAAQPLPASAVVAVVGTGAMGAGIAQVAAAAGHV
                     VKLLDNRPGAAAKAIEGIRAQFGKLADKGKMSTEAAQAAAERLIAVDQLAELADAALV
                     VEAIVENLEAKQKLYG"
     gene            complement(350..1144)
                     /gene="paaG"
                     /locus_tag="PKV97_12855"
     CDS             complement(350..1144)
                     /gene="paaG"
                     /locus_tag="PKV97_12855"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HNB06815.1"
                     /translation="MEYNNIQFAVEAGVARLTLNRPDKLNSFTGEMHAELRDALDRVH
                     ADKAARVLVLTGAGRAFCAGQDLADPDMASVGGKMPDIGNVVEKNYKPLVLRLQNLRV
                     PTIAAVNGIAAGAGASVALACDLVVAAKSASFLQAFSKIGLVPDTGGTWFLPQRVGMA
                     RAMGLAMLADKLPAEKAADWGLIWAAYDDAEFAAKVDALAAQLAAMPTKALVRTRQAM
                     HAAAGHTLEQQLSFEGGFMRELGWSPDYAEGVAAFMEKRAPKFTGE"
     gene            complement(1155..2822)
                     /gene="paaN"
                     /locus_tag="PKV97_12860"
     CDS             complement(1155..2822)
                     /gene="paaN"
                     /locus_tag="PKV97_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HNB06816.1"
                     /translation="MPHPLLEKHRATLESALDAIATRGYWSAFPEMPSPKLYGEAAPD
                     EGKRAFEAHLGKPFELGQPGQTGWHGGESSPYGVALDVRYPVCDPETLIAAGLEAMKG
                     WQAVGADGRTGICLEILQRLNKQSFEIAHAVMMTTGQGWMMAFQAGGPHAQDRGLEAV
                     TYAWREQSFVPAETTWEKPQGKNPALVMKKHFQIVGRGVGLVIGCGTFPTWNTYPGLF
                     AALATGNAVIVKAHSNAILPAAITVRTIRTVLAENGIDPNLVSLCVATQRSVTQALAT
                     HPAVQSVDFTGSNVFGQWLIDNCRQAQVYAELAGVNNIVIDSTDSYKAMLGNLAFTLS
                     LYSGQMCTTSQAIFVPAGGIDTEDGHKSYDEVCADLARAVERFLSKPEVAHAVLGAIQ
                     SADTAERIDIANSGALGKVVLASQKLDNPEFPGAKVRTPVLLACDAADEKAYMEERFG
                     PISFIVKVADTAAGIALSERVVRTHGALTVGLYSTRQDVIDAMTEATWRGKVALSINL
                     TGGVFVNQSAAFSDYHGTGGNPSANASYSDSAFVANRFRVVQRRYHV"
     gene            3152..4246
                     /gene="paaX"
                     /locus_tag="PKV97_12865"
     CDS             3152..4246
                     /gene="paaX"
                     /locus_tag="PKV97_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HNB06817.1"
                     /translation="MPAESAWRSSSSLAYASAAASKARSGARRICPESIDFLPPTAAR
                     TSSSVTTSIQDRLEAFRKQDRVQAGSLIISVFGDAVLPRGGRIWLGSLIRLLEPLGLN
                     ERLVRTSVFRLARDEWLVSEPCGRRTDYMLTPSGLQRIEEASRRIYASAAQGWDRRWR
                     LILAVGELAAKDRERLRRALFWQGFGALDGDCFVHPSADLITAFDALVGEGLGGLLPR
                     LKPLMAADAALGAAASDADLVHAAWNLERLAQTYREFVDCYQPVLAQLRDGASESGDE
                     NAFLMRMLLIHDYRRLLLRDPGLPDVLLASDWPGQRARLLCRELYRRLLVPSERHIDA
                     HLRLADGRTPEASPRLLARFQDADPLRGAA"
BASE COUNT          614 a         1399 c         1510 g          756 t
ORIGIN      
        1 ccgtacagct tctgcttggc ttcgaggttc tcgacgatgg cctcgaccac cagtgcggcg
       61 tcggcgagct cggccagctg gtcgacggcg atcaggcgtt cagcggccgc ctgcgcggct
      121 tcggtgctca tcttgccctt gtcggcgagc ttgccgaact gggcacggat gccttcgatc
      181 gccttggcgg cggcgcccgg acggttgtcg agcagcttga ccacgtggcc ggccgcggcc
      241 gccacctggg cgatgccggc gcccatggcg ccggtgccga ccacggcgac gacagccgag
      301 gcgggcagcg gctgcgcggc ggacttggag atctgggtgt cggtcacgct cattccccgg
      361 tgaacttcgg tgcgcgcttt tccatgaagg cggcgacgcc ttcggcgtag tcggggctcc
      421 agcccagctc gcgcatgaag ccgccctcga aggagagctg ctgctccagc gtatggccgg
      481 ccgcggcgtg catggcctgg cgggtgcgca ccagggcctt ggtcggcatg gcggcgagct
      541 gcgcggcgag cgcgtccacc tttgcagcga actcggcatc gtcgtacgcc gcccagatca
      601 ggccccagtc ggcggccttc tcggcgggca gcttgtcggc gagcatcgcc aggcccatcg
      661 cgcgcgccat gccgacgcgc tgcggcagga accaggtgcc gccggtgtcg ggtacgagtc
      721 cgatcttgct gaaggcctgc aggaaggagg ccgacttcgc cgccaccacc agatcgcagg
      781 cgagcgccac gctggcgccg gcgccggcgg cgatgccgtt gaccgcggcg atggtgggca
      841 cgcgcaggtt ctgcaggcgc agcacgagcg gcttgtagtt cttttcgacg acgttgccga
      901 tgtcgggcat cttgccgccc accgacgcca tgtcgggatc ggccagatcc tggccggcgc
      961 agaaggcgcg gccggcgccg gtgagcacca gcacgcgcgc ggccttgtcg gcgtggacgc
     1021 ggtcgagcgc gtcgcgcagc tcggcgtgca tctcgccggt gaagctgttg agcttgtcgg
     1081 ggcggttcag cgtcaggcgg gcgacgcccg cctccactgc aaactggatg ttgttgtatt
     1141 ccacggacgg atcctcagac gtggtagcgg cgctggacga cgcggaagcg gttggcgacg
     1201 aaggcggaat ccgaatacga ggcgttggcc gacgggttgc cgccggtgcc gtggtaatcg
     1261 gagaaggccg ccgactggtt cacgaacacg ccgccggtga ggttgatcga cagcgcgacc
     1321 ttgccgcgcc aggtggcttc ggtcatcgcg tcgatgacgt cctgcctcgt ggagtagagc
     1381 ccgacggtga gcgcgccgtg ggtcctgacc acacgctcgg agagcgcgat ccctgcggcg
     1441 gtgtcggcga ccttgacgat gaagctgatc gggccgaagc gctcttccat gtaggccttc
     1501 tcgtcggcgg cgtcgcaggc gagcagcacc ggggtgcgca ccttggcgcc cgggaactcg
     1561 gggttgtcga gcttctgcga ggccagcacg accttgccca gcgcgccgct gttggcgatg
     1621 tcgatgcgtt cggcggtgtc ggcggactgg atcgcgccga gcacggcgtg ggcgacctcg
     1681 ggtttggaca ggaagcgctc gaccgcacgg gcgaggtcgg cgcagacctc gtcgtagctc
     1741 ttgtggccgt cctcggtgtc gatcccgccc gcgggcacga agatcgcctg cgaggtggtg
     1801 cacatctggc cggaatacag cgagagcgtg aaggcgaggt tgcccagcat cgccttgtag
     1861 gagtccgtcg agtcgatgac gatgttgttc acgccggcga gctcggcata gacctgggcc
     1921 tggcggcagt tgtcgatcag ccactggccg aacacgttgc tgccggtgaa gtcgaccgac
     1981 tgcaccgccg ggtgggtggc gagcgcctgg gtgacgctgc gctgggtggc cacgcacagg
     2041 ctcaccaggt tggggtcgat gccgttctcg gcgagcacgg tgcggatcgt gcgcacggtg
     2101 atcgccgccg gcaggatggc attgctgtgc gccttgacga tgaccgcgtt gccggtggcg
     2161 agcgcggcga acaggcccgg gtaggtgttc caggtcggga aggtgccgca gccgatcacc
     2221 aggcccacgc cgcggccgac gatctggaag tgcttcttca tgaccagcgc cgggttcttg
     2281 ccctggggct tctcccaggt ggtctcggcc ggcacgaagc tctgctcgcg ccaggcgtag
     2341 gtcacggcct cgaggccgcg atcctgggcg tgcgggccgc cggcctggaa ggccatcatc
     2401 cagccctggc cggtggtcat catgaccgcg tgggcgatct caaagctctg cttgttcagg
     2461 cgctggagga tttccaggca gatgccggtg cggccgtcgg cgcccaccgc ctgccagccc
     2521 ttcatcgcct cgagcccggc ggcgatcagc gtctccgggt cgcacaccgg atagcgcacg
     2581 tcgagcgcca cgccataggg cgagctctcg ccgccatgcc agcccgtctg gcccggctgc
     2641 ccgagctcga acggcttgcc caggtgcgcc tcgaaggcgc gcttgccctc gtccggcgcc
     2701 gcctcgccat agagcttggg gctcggcatc tcggggaagg cagaccagta gccgcgcgtg
     2761 gcgatggcgt cgagggcgct ttcgagggtg gcgcggtgct tttcgagcaa ggggtggggc
     2821 atgggtctct ccgagtgggt ggtcttgtgc ttcaaacgat acaaatcgat ttgataaatg
     2881 tcaatactag gtatctattc tggagggggt gatcacctca agttttgata ggcgaaacac
     2941 gtttagatat aaggaagtct tttataaaca gtgggatatg tgaaaattat cggggtgttc
     3001 cggcagccgt agccagtcga aaaacccggg gcgtccgctg ctctggactt aatgtgatac
     3061 aagaagacat agaaaaatct agtataagta tcgtattaat ctccttgctg ccttgccctc
     3121 ccgcgcactc gcccgcacgc atccaggctt cgtgcccgcg gaatcggcgt ggcgctcgtc
     3181 ctcgagcctt gcgtacgcga gtgcggcggc ctcgaaagcg cgctccggcg ctaggcgcat
     3241 ctgcccggaa tcgatagact tcttgccccc aaccgcagcg cgaacaagct cgtccgtgac
     3301 cacttccatc caagatcgcc ttgaagcgtt ccgcaagcaa gatcgcgtgc aggccgggtc
     3361 gctgatcatt tccgtcttcg gcgacgccgt gctgccccga ggcggacgca tctggctggg
     3421 cagcctgatc cgcttgctcg agccgcttgg gctcaacgag cgtctcgtcc gcacatcggt
     3481 gtttcggctt gcgcgcgatg aatggctggt cagcgagcct tgcggccgcc gtacggacta
     3541 tatgctgacg ccttccggtc tgcagcgtat cgaggaggcg tcgcgccgca tctacgcttc
     3601 cgctgcgcag ggctgggatc gtcgctggcg gctgatcctc gccgtcggcg agcttgcggc
     3661 caaggaccgc gaacgcctgc gcagggcgct gttctggcag ggcttcggcg ccctcgacgg
     3721 ggactgcttc gtgcatccga gcgcggacct catcaccgcc ttcgacgcgc tcgtcgggga
     3781 ggggttgggt gggctgctgc cgcggctcaa gcccctgatg gctgcggatg cggccctggg
     3841 tgcggcggcc agcgacgccg acctggtgca tgcggcatgg aatctcgagc gcctggcgca
     3901 gacctatcgc gaattcgtcg actgctacca gcccgtgctg gcccagctcc gcgatggcgc
     3961 cagcgagtcc ggggatgaga acgccttcct catgcggatg ctgctgatcc acgactaccg
     4021 ccgcctgctg ctgcgcgatc ccggattgcc cgacgtgctg ctggcgagcg actggccggg
     4081 gcagcgtgcg cggctgctgt gccgcgaact ctaccgtcgg ctgctggtgc cgtccgagcg
     4141 ccatatcgac gctcacctcc gcctcgccga cggccgcacg cccgaggcgt cgccccgcct
     4201 tctcgcgcgc ttccaggacg ccgacccgct gcgcggcgcc gcctgagcgc cgccagcctc
     4261 ttcccgccgg tcggcgcgc
//
LOCUS       DAOQNN010000278         4256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13165_length_4256_cov_9.928826, whole genome shotgun sequence.
ACCESSION   DAOQNN010000278 DAOQNN010000000
VERSION     DAOQNN010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13165_length_4256_cov_9.928826"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..738
                     /locus_tag="PKV97_12870"
     CDS             328..738
                     /locus_tag="PKV97_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNB06818.1"
                     /translation="MAGRKKNLPLVYSCSGCSSAAQMANHVALRLDREGEAEMSCIAG
                     VGGNVPHLVNIARSGRPILALDGCALECTRSSLAERGVRPAAHLLLNEQGVKKRYATD
                     FDREEAERVFEQARAAVRGLRATPATAMTGGTTD"
     gene            complement(744..1331)
                     /locus_tag="PKV97_12875"
     CDS             complement(744..1331)
                     /locus_tag="PKV97_12875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNB06819.1"
                     /translation="MKLPRIVVMPGSRRREALSRRVAQACAQAVREAGAEVELVDLDD
                     YPAPLYDGDLEAASGLPEGIVRLQRVLHASDGLLLVNPEYNGSLTPLLKNTLDWCSRP
                     NPADAERSGGKVYAGRAAAVVGTSPGALGGMRVLFHVRDVLGYLGMQVIPQQVAVGQA
                     GEAVGADGRLLDAARQGMLEGLAEALVDTARRLRA"
     gene            1516..1953
                     /gene="trxC"
                     /locus_tag="PKV97_12880"
     CDS             1516..1953
                     /gene="trxC"
                     /locus_tag="PKV97_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HNB06820.1"
                     /translation="MSDALHLVCPHCDAVNRVPATRLAEAPNCGRCGKPLFTAHPVEL
                     DAANFDRHVGRSDVPVLVDFWAPWCGPCRTMAPAFAQAAGMLEPQLRLAKLDTEAHQA
                     LAARFGIRSIPTLALFRGGREIARQAGVMSAADIVRWVRGQLG"
     gene            complement(1962..2300)
                     /locus_tag="PKV97_12885"
     CDS             complement(1962..2300)
                     /locus_tag="PKV97_12885"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB06821.1"
                     /translation="MPADLNPSGDVFGGWIMSMVDIAGAVPAIRVARSKVATVAVDSF
                     VFKQPVSTGDIVSFHAEVVRVGRTSITVDVEVYAQRHPENPVVVKVTEARLTYVAVDE
                     FRAKRTIGAG"
     gene            complement(2478..2702)
                     /locus_tag="PKV97_12890"
                     /pseudo
     CDS             complement(2478..2702)
                     /locus_tag="PKV97_12890"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
     gene            complement(2849..>4256)
                     /locus_tag="PKV97_12895"
     CDS             complement(2849..>4256)
                     /locus_tag="PKV97_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNB06822.1"
                     /translation="LPAVQLAELAAAARLLELPARTLVHGCGEPFDEAYVLCSGTVVR
                     FRELDGDARKVVELVHTPQLLGCGEFFGASRHESGCETASACALIALDAGVLRAVAER
                     SLVLTRRMLQAVAARLCEVEFDVAGRHASRTSAQRILDYLVELAGGSLALAGETTVEL
                     ASTKKVLAARIGITPEAFSRSLRELADKGVIVVDKRLIHIQNAATLDTGAGEQPQRLS
                     FSRRNKGARRDETMSVGELINLCGRLRLQVQRLAIDWVLIGHGVAPVEMQLRLRQDIA
                     EFERSLAVLQAASLAPELGEALEDVGRVWADFRDALGAAERPPAGAARVLQLSEDFIT
                     AADFLNAVAGGFAASGSLYMVNVAGRNRMLSQRVAKFFLFEGFEGCPDPQAQIAASCR
                     AFEHNLQRLRENAGELPELVAQIDATAALWARFIAALDPAIRQPHRIGRVRAVLDEGE
                     RLLRYTNTLVKLFERLAH"
BASE COUNT          665 a         1570 c         1462 g          559 t
ORIGIN      
        1 agctgcggcc ggcgcagcgg gggccgcagc cgctgccgga gccgcggggg ccttggccgg
       61 cgccgcggga gtgccgctac cggagttgcc acaggcagcc agcagaacgg acgccgcagc
      121 ggcgatcagg aagggaaact tggtcatgcg tattctccga tttgtcgtgg tccgtcgcag
      181 agcgtctccg cgaatgcatt gcacactagc ggcaaaccct cacaagttga ttgatagcgg
      241 tcaattcacc ccgaacacgg tccaccgggt gaactgccgc agcatagtaa actattcgtc
      301 ccaacatgcc cgcccgagga ccccgccatg gccggccgca agaagaacct gccgctcgtc
      361 tattcctgct cgggctgctc cagcgcagcg cagatggcca accacgtggc gctgcgcctg
      421 gatcgcgagg gcgaggccga gatgtcctgc atcgccgggg tcggcggcaa cgtcccccat
      481 ctggtgaaca tcgcccgctc cgggcgaccg atcctcgcgc tcgacggctg cgcgctcgaa
      541 tgcacgcgca gcagcctggc cgagcgcggc gtccgacccg ccgcccacct gctgctcaac
      601 gagcaggggg tcaagaagcg ctacgccacg gatttcgatc gcgaagaggc cgagcgcgtt
      661 ttcgagcagg cccgcgcagc ggtgcgcggc ctgcgtgcga cgcccgccac cgcgatgacg
      721 ggcgggacga cggactgaag ccctcaggcg cgcaggcgcc gcgccgtgtc caccaacgcc
      781 tccgcaagcc cctccagcat gccctgccgg gcggcatcga gcaggcgtcc gtcggcgccg
      841 accgcctcgc cggcctggcc caccgcgacc tgctgcggaa tgacctgcat gccgaggtag
      901 ccgagcacgt cgcgcacgtg gaacagcacc cgcatgccac cgagcgcgcc cggcgaggtt
      961 cccaccaccg ccgccgcccg cccggcatac accttgccgc cggagcgctc ggcgtcggcc
     1021 gggttgggcc gcgagcacca gtcgagggtg ttcttgagca agggcgtgag cgagccgttg
     1081 tactcgggat tgaccagcag caggccgtcg ctcgcatgca gcacccgctg caggcgcacg
     1141 atgccctcgg gcaggccgga ggcggcttcg aggtcgccgt cgtagagcgg cgcggggtaa
     1201 tcgtccagat cgacgagctc gacctcggcc cccgcctccc gcacggcctg cgcgcacgcc
     1261 tgcgccacgc gacgcgacaa ggcctcgcgc cggcggctac cgggcatcac cacgattcgg
     1321 ggcagcttca tcggcgcttc tccttcacaa cgagatgccg gcattatccg ccggatcggc
     1381 ggtgcgaacg cgatcacgcc gaatcgcggc cacatgccgc ccccaccccc agccgcatcg
     1441 acggacaccg gctctcttga atcccgccgc ttcgcctcga catcgggaga atccgaaatg
     1501 cccgggagcc ccgccatgtc cgacgccctg cacctcgtct gcccgcactg cgacgctgtc
     1561 aaccgtgtcc ccgccacccg cctcgccgaa gcaccgaact gcggcaggtg cggcaagccg
     1621 ctgttcaccg cccacccggt ggagctcgac gcggcgaact tcgaccgcca tgtcggccgc
     1681 agcgacgtcc cggtgctggt ggacttctgg gcgccgtggt gcggcccctg tcgcacgatg
     1741 gcgcccgcct tcgcgcaggc cgcgggcatg ctcgaaccgc agctacgcct cgccaagctc
     1801 gacaccgaag cccaccaggc cttggcggca cgcttcggca tccgcagcat ccccaccctg
     1861 gcgctgtttc gcggcggccg cgagatcgcc cgccaggccg gcgtgatgag cgcggccgac
     1921 atcgtccgct gggtgcgcgg ccagctcggc tgaaagcggg ctcagcccgc cccgatcgtg
     1981 cgcttggcgc ggaattcgtc caccgcgaca taggtcaggc gcgcctcggt caccttcacc
     2041 accaccgggt tctccgggtg gcgctgggcg tagacctcga cgtccacggt gatcgaggtg
     2101 cgccccacgc gcaccacctc ggcgtggaag ctgacgatgt cgccggtcga caccggctgc
     2161 ttgaagacga aggagtccac cgccacggtg gcgaccttgg agcgcgcgac gcggatcgcc
     2221 ggcaccgcgc cggcgatgtc caccatcgac atgatccagc caccgaacac gtcgcccgag
     2281 gggttgaggt cggccggcat gggcacgacc cgcagcacga gctccttgtc caggggcgga
     2341 cgctcgccgc cggggggcag ggtcgactcc gggcccacca tgcgcttgca gcccgcgcgg
     2401 tcgacggcga cgtaagtcat gcgcgcctcg gtcaccttga ccaccaccgg atccgccgga
     2461 tggcgctcgg caaacacgtg cacgtcgacc gtgatcgagg acttgcccac cgcgaccacg
     2521 tcggcgtgga agctgacgat gtcgccgacc gagatcggct gcctgaagac gaaggaattc
     2581 accgccatcg tgaccacgtg cgagcgcgcg cggcggatcg cgggcagggc gccggcgatg
     2641 tcgaccatcg ccatgaccca accgccgaac acgtcgccgg cggagttgag gtcggccggc
     2701 atcggcatca ggcgcaacgc cggctcggtg gcgggcggcg tcgtcgggtg ggaggggctg
     2761 gaggctgcgc tcatgggttg ctccacggat cgaggggaaa agggcgcccc gtgccggaca
     2821 ggcggggcgc ggaatcagcg gccggatttc agtgcgcgag gcgctcgaag agcttgacca
     2881 gggtgttggt gtagcgcaac aggcgctccc cctcgtcgag caccgcgcgc acccggccga
     2941 tgcgatgagg ctggcggatc gcggggtcga gcgcggcgat gaagcgcgcc cacagggcgg
     3001 ccgtggcgtc gatctgcgcc accagctcgg gcagctcgcc ggcattctcg cgcaagcgct
     3061 gcaggttgtg ctcgaaggcc cggcacgagg cggcgatctg cgcctgcggg tcggggcagc
     3121 cctcgaagcc ctcgaagagg aagaacttcg ccacccgctg cgacaacatg cggttgcgtc
     3181 cggcgacgtt gaccatgtac aggctgccgg aggcggcgaa gccgccggcc acggcgttga
     3241 gaaaatccgc cgcggtgatg aagtcctcgc tcagctgcag cacccgcgcc gcgcccgccg
     3301 gcgggcgctc ggccgcaccc agcgcatcgc gaaaatccgc ccacacccgg cccacgtcct
     3361 ccagcgcctc gccgagctcg ggggcgaggc tggcggcctg caggaccgcg aggctgcgct
     3421 cgaactcggc gatgtcctgg cgcaggcgca gctgcatctc gaccggcgcc acgccatgcc
     3481 cgatcagcac ccagtcgatc gccagacgct gcacctgcag gcgcaggcgg ccgcacaggt
     3541 tgatcagctc accgaccgac atcgtctcgt cgcggcgcgc acccttgttg cggcgcgaga
     3601 agctcaggcg ctgcggctgc tcgcccgcac cggtgtccag ggtggcggcg ttctggatgt
     3661 ggatcaggcg cttgtcgacc acgatcacgc ccttgtcggc aagctcgcgc agactgcgcg
     3721 agaaggcctc gggggtgatg ccgatgcgcg cggcgagcac cttcttggtc gaggccagct
     3781 ccaccgtggt ctcgccggcg agcgccaggc tgccgccggc gagctcgacc agataatcga
     3841 ggatgcgctg cgcgctggtg cggctggcat ggcgcccggc gacgtcgaac tcgacctcgc
     3901 acagccgcgc cgccaccgcc tgcagcatgc gccgggtcag caccaggctg cgctcggcca
     3961 ccgcgcgcag cacgccggcg tcgagcgcga tcagcgcgca ggcgcttgcc gtctcgcagc
     4021 ccgattcatg acggctggca ccgaagaact cgccgcagcc gagcagctga ggggtgtgca
     4081 ccagctcgac caccttgcgc gcgtcgccgt cgagctcgcg aaagcgcacc accgtcccgc
     4141 tgcacaacac gtatgcctcg tcgaagggct cgccgcagcc atgcaccagg gtgcgcgccg
     4201 gcagctcgag caggcgcgcc gcggcggcga gctcggccag ctgcacggcg ggcagg
//
LOCUS       DAOQNN010000279         4241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13233_length_4241_cov_6.579312, whole genome shotgun sequence.
ACCESSION   DAOQNN010000279 DAOQNN010000000
VERSION     DAOQNN010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13233_length_4241_cov_6.579312"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1004..1960)
                     /locus_tag="PKV97_12900"
     CDS             complement(1004..1960)
                     /locus_tag="PKV97_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HNB06823.1"
                     /translation="MRTLWNWEHRDPTATLPKWGRKPHSPQAHRDAYHAVRIVMKAQG
                     KTIGEGTALRALQGAVPTLLVRWALKVRKAWLRRRDAHRCAAQRMSIVPVAPGVLGSL
                     DAMQLGRVEHEPVKAEQVVDVLSTKTLAAEIVWSPSGADVVRMLERLRDSDEGLPLAF
                     LLDRGPENKNREVARFAEEHHVVLIYNAPHTPEHNAPVERKNRDVRGVSGLRASTRLA
                     SLDEARCRFAAATRVLDHARLRAKHGYATAAVRDRIGRVRYTPALREALWRDVKAAQE
                     RARSDCARARDRPRAEREAALAVLDVYGMIERYRGGVRVRSA"
     gene            complement(1963..>4241)
                     /locus_tag="PKV97_12905"
     CDS             complement(1963..>4241)
                     /locus_tag="PKV97_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HNB06824.1"
                     /translation="EAQVDEQEQDPRWLDEERDGEEDGEGQDAPLSRGTAERSPEGAS
                     AAVPRESGASGNRYSVGEKLDYLRRFVQSGKTLVEFCRRERLNTKSFCDWRRQFDALG
                     EAGLRPKPNLRNTGGHSGRVIPADERRALVESFLRMKLSQAVFARTYGISVSSLSKWL
                     RRYHAHGPKGLEPGKRGRKPGSSGGARIALPIQAEILNTKRRFPDFGMRKVRDYMKRF
                     FGFKVSAGGVRATLEREGIAPLPMLKKRRRGPDRIRRFERATPMDLWQSDITSYVLTR
                     NSRRVYLTVFLDDRSRYVVAWSLATRQTSAFVCDTLLEGIASFGKPKEVLTDQGRQYF
                     AWRGKSEFQKLLDKEGIRHVVARSHHPQTVGKCERLWKTVGTEFWERAQPQDLDDARE
                     RLGHFFSHYNHFRPHQSLEGMTPADRFFGAETELRAMLEAQQAEHELALAIDQPPRKP
                     VFLIGQVGDQKVSMRALDGRIEFGTEGGAIEAMALKDAGIPQTKPEAKPANPTTMNPE
                     NEHATQHGVEHGTEHDEPESAHEQHGGGAAIADEPAEPAAGGSDAAGVAGDLEADAPR
                     AQAPHASAAASGDLDPGPVGSGESGGVGAGEGDRALDHRDVDRQDEQGGGGTPAEDVA
                     APGVAAVAAGGVGRDCGAVDPAAHAAAAEVCAACGRPRGAGEAAGAVERSGALAEGSA
                     APEAGALGDRAGERAVAPIPGAEKVGESRCEEVAPSTGREPSGAGLAPSTASESQPNC
                     ERERFDAKSDERSQRATA"
BASE COUNT          585 a         1538 c         1318 g          800 t
ORIGIN      
        1 actaacgctc gtatcgctgg tggctttctc gcgtgcccag catgccatta ctagtccgac
       61 ggttaccgcg gcggacgaag ggatgattcg cttgaacggc acggtaagtt tctccactct
      121 aatctagatc catcgacggg cggcgggcgc gcgagcttgc gtcgagtcat acaaatacca
      181 gaacttcgat gctacgcttt ctttacatct gtgagcaagc tcaattcccc gcgatgtgca
      241 cggttggggt ttcgcccaag cgtacgcttc tgcgcccatc tgatctcgcg ctcgaggagg
      301 ggcagctccg gatcgggcgt cgccgggtga ttctcgcggc gtctccggtc acgtggaacg
      361 cttccaaggg ttccggtagc gggtgcctca catgggcatc ggctttcgag atcgggggag
      421 gccgcccttg caatcgccga gcgctgcgcg ccccggttct cgtgcccgag tacaagttcg
      481 cgcgcattcc tcgcaatccg gcctgcaacg ctgcgtgtgc taactgcttt gcgttgcgat
      541 gctctccagg agtctgtctt tgcttcggat ttgttagtgc tgctttctgg tcgccaaagg
      601 agcgtggcaa cagtcgctca cggctcgagc gcggttcgcg ggaggcgtcg ccgcgtctac
      661 acttgactca gagcgaacgc gcacgccaac cagcaacctt cgagtgagcc gtcggtacct
      721 tctgcgagat cttgaccaag agcgttatag atctggatat cgtctcctcg gccgctccag
      781 tcttcggcac ctgctgcgcc tccagttgcc cacgaagcag ctatctcagc tccttcccag
      841 acaacccctc cggcggcgga tatggctttg gcagccgcgt aagccgcagg agaccctcca
      901 agaactgttg caatcttgtg agttactcgg cagtggtgaa cgcagtgcag ttggccccag
      961 tactcctcat cgctctgtgt agtgcacacg aaatgatttc cgcttaagca ctccggactc
     1021 gcaccccgcc tcgataccgt tcgatcatcc catacacgtc cagcactgcc aatgcggcct
     1081 cccgttctgc ccgcggccgg tctcgcgccc ttgcgcagtc tgagcgcgcc cgctcttggg
     1141 cggccttcac gtcgcgccac agcgcctcac gcagcgcggg agtgtaacgc acccgtccga
     1201 tccggtcacg cacagccgcg gtcgcgtagc cgtgcttggc gcgcaagcgg gcgtggtcga
     1261 gcacgcgcgt cgcggcggcg aagcggcagc gcgcctcgtc gagcgaggcg agcctggtgc
     1321 tggcgcgcag tccggacact ccgcgcacgt cacggttctt gcgctcgacc ggcgcgttgt
     1381 gctcgggcgt gtgcggcgcg ttgtagatca agacgacgtg gtgctcctct gcgaagcgcg
     1441 cgacctcgcg gttcttgttc tcgggcccgc ggtcgagcag aaacgcgagc ggcaagccct
     1501 cgtcgctgtc gcgcagccgc tcgagcatgc gcaccacgtc ggcgccgctc ggcgaccaca
     1561 cgatctccgc ggcgagcgtc ttggtcgaca gcacgtcgac cacctgctcg gccttgaccg
     1621 gctcgtgctc gacgcggccg agctgcatcg cgtcgagcga gcccagcacg ccgggcgcga
     1681 ccggcacgat gctcatgcgc tgtgccgcgc accggtgcgc gtcacggcga cgcagccatg
     1741 cctttcggac cttgagcgcc cagcgcacga gcagcgtcgg cactgcgccc tgcaaggcgc
     1801 ggagcgcggt gccttcgccg atcgtcttgc cttgtgcctt catcacgatg cgcaccgcat
     1861 ggtacgcgtc acggtgcgcc tgcggactgt gcggcttgcg gccccacttc ggcagcgtcg
     1921 ccgttgggtc ccggtgctcc cagttccaca gggtccgcac cgttacgccg tggctcgttg
     1981 cgaccgctcg tcgctctttg cgtcgaatct ctctcgttcg cagttgggtt gcgactcgct
     2041 tgctgtcgac ggcgcgagtc cggcaccgct tggctcgcgg cccgtgctcg gcgcgacttc
     2101 ttcgcatcga gactctccga ctttctcagc accgggaatt ggcgcaacag cgcgttcacc
     2161 tgctcggtcg ccaagagctc ctgcttcagg cgccgcactt ccttccgcaa gagctccgga
     2221 tcgctcgacg gcgccggccg cctcaccagc gcctcgaggc cgcccgcaag cggcgcagac
     2281 ctccgccgcg gccgcgtgcg cggctggatc aacagccccg caatcacgcc cgacaccgcc
     2341 tgctgcgaca gctgccacac ccggagcggc gacatcttca gctggcgtgc cacctccgcc
     2401 ttgctcgtct tgccgatcca catctcgatg atcaagagcg cgatctcctt cgcccgcgcc
     2461 aactcctccg gactcgccgc ttcccacagg ccccggatcg agatcgccgg acgcggcagc
     2521 actcgcgtgc ggcgcttgcg ctcgtggcgc gtccgcttcg agatcgccgg cgactccggc
     2581 cgcgtcagat ccgccggctg cgggctcggc aggttcgtct gcgatcgcgg cgccgcctcc
     2641 gtgctgttcg tgggcggact cgggctcgtc gtgttcggtg ccgtgctcga cgccgtgctg
     2701 tgtcgcatgc tcgttctccg ggttcatggt ggtggggttg gctggtttcg cttcgggttt
     2761 cgtttgcggg atccccgcgt ccttcagcgc catcgcctcg atcgcgccgc cctcggtgcc
     2821 aaactcgatc cgcccgtcga gcgcgcgcat cgacaccttc tggtcgccga cctgcccgat
     2881 caggaaaacc ggcttcctcg gcggctgatc gatcgcgagt gcgagctcgt gctcggcctg
     2941 ctgcgcctcg agcatcgcgc gcagctcggt ctccgcgccg aagaagcggt ccgccggcgt
     3001 catcccctcg aggctctggt gcggccgaaa gtggttgtag tgcgaaaaga agtggcccaa
     3061 gcgctcgcgc gcgtcgtcga ggtcttgcgg ctgcgcgcgc tcccaaaact ccgtgccgac
     3121 cgtcttccag agccgctcgc acttgcccac cgtctgcggg tggtgactgc gcgcgaccac
     3181 gtggcggatg ccctccttgt cgagcagctt ctggaactcg ctcttgccgc gccacgcgaa
     3241 gtactgccgg ccctgatcgg tcagcacctc cttcggcttg ccgaagctcg caatgccttc
     3301 gagcagcgtg tcgcacacaa acgcgctggt ctgccgcgtc gcgagcgacc acgcgaccac
     3361 gtagcggctg cgatcatcga gaaacaccgt caagtacacg cgccgtgagt tgcgcgtcag
     3421 cacgtagctc gtgatgtcgc tctgccagag gtccatcggc gtggcccgct cgaagcgccg
     3481 aatccggtcc ggtccgcgcc gccgcttctt cagcatcggc agcggcgcga tgccctcacg
     3541 ctcgagcgtc gcgcgcacgc ctcccgcgct gaccttgaag ccgaagaagc gcttcatgta
     3601 gtcgcgcacc ttgcgcatcc cgaagtccgg gaagcgccgc ttggtgttca agatctccgc
     3661 ctggatcggc agcgcgatcc gcgcgccccc gctgctgccc ggcttccggc cgcgcttgcc
     3721 gggctcgagc cccttcgggc catgcgcgtg gtagcgccgc agccacttgc tgagcgacga
     3781 caccgagatc ccgtaggtcc gtgcgaacac cgcctggctc agcttcatcc gcaggaacga
     3841 ctcgaccagc gcgcggcgct cgtcggccgg gatcacccgg ccggagtgac cgccggtgtt
     3901 gcgcagattg ggcttcggcc gcagccccgc ctcgccgagc gcatcgaact gccgacgcca
     3961 atcgcagaac gacttggtgt tgaggcgctc gcgccggcag aactcgacca gcgtcttgcc
     4021 gctctgcacg aagcggcgca ggtagtcgag cttctcgccg acgctgtagc gattccccga
     4081 cgctccgctc tcgcggggga cggctgcgct cgccccttcg gggcttcgct ccgccgtccc
     4141 ccgcgagagc ggagcgtcct gcccttcccc gtcctcctcc ccgtcccgct cctcgtcaag
     4201 ccaacgcggg tcctgctcct gctcgtccac ctgcgcctcc t
//
LOCUS       DAOQNN010000280         4231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13284_length_4231_cov_8.987548, whole genome shotgun sequence.
ACCESSION   DAOQNN010000280 DAOQNN010000000
VERSION     DAOQNN010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13284_length_4231_cov_8.987548"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..444
                     /gene="ureA"
                     /locus_tag="PKV97_12910"
     CDS             142..444
                     /gene="ureA"
                     /locus_tag="PKV97_12910"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HNB06825.1"
                     /translation="MELSPREKDKLLVFTAGLLAERRRARGLKLNYPEAVALITCAIL
                     EGARDGRTVAELMGYGATLLTRADVMAGVAEMIPDIQVEATFPDGTKLVTVHNPIV"
     gene            456..809
                     /locus_tag="PKV97_12915"
     CDS             456..809
                     /locus_tag="PKV97_12915"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HNB06826.1"
                     /translation="MIPGELFVADGDIELAAGRATRTLVVSNTGDRPIQVGSHYHFAE
                     TNSALDFDRAAARGFRLNIAAGTAVRFEPGQWRTVELVALDGEREVYGFNGLVMGRLD
                     EPATAPAAHRTQGSL"
     gene            809..2560
                     /gene="ureC"
                     /locus_tag="PKV97_12920"
     CDS             809..2560
                     /gene="ureC"
                     /locus_tag="PKV97_12920"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HNB06827.1"
                     /translation="MATISRRAYAEMFGPTVGDRLRLADTELVIEVEKDFTIYGEEVK
                     FGGGKVIRDGMGQGQGVSADVADTIVTNALIIDAVAGIVKADVGLKDGRIWRIGKGGN
                     PDIQPGVTIPIGAGTEVIAGEGMILTAGGIDSHIHWICPQQIEEALSSGVTTMLGGGT
                     GPATGTFATTCTPGPWHIHRMLEAAEAFPMNLGFFGKGNASLPAPLKEQVQAGVIGLK
                     LHEDWGTTPAAIDNCLTVAEQMDIQVAIHTDTLNESGFVETTLAAFKDRTIHTFHTEG
                     AGGGHAPDIIRAISRPNVLPSSTNPTRPYTVNTIDEHLDMLMVCHHLDPSIAEDVAFA
                     ESRIRRETIAAEDILHDTGAFSMMSSDSQAMGRVGEVVIRTWQTAHKMKLQRGWLAPR
                     RSAAAAVPDAVAVVEGSTANDNFRVKRYIAKYTINPALTHGIAHEVGSIEPGKLADLV
                     LWRPAFFGVKPSLVIKGGMIAAAAMGDPNASIPTPQPVHWRPMFGSFGRALKCAVTFV
                     SQAALHNAAVAALGLQKPLVAVKGCRTLTKADMVLNDATPQIEVDPETYVVRADGEHL
                     ACEPATELPLAQRYFLF"
     gene            2598..3146
                     /gene="ureE"
                     /locus_tag="PKV97_12925"
     CDS             2598..3146
                     /gene="ureE"
                     /locus_tag="PKV97_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HNB06828.1"
                     /translation="MPMTAETLADFVMPAPATDVLLIEQFHEGDEPASEVLELDFGAR
                     TKSRLRTRLASGAEVGLFLPRGTILRGGQKLRARDGRIVEVRAAAEDLLEARCASAVA
                     LARAAFHLGNRHVAVEVGHDEAGDWLRLQADHVLEGMLVGLGALVSALRAPFEPEAGA
                     YAPGHQHPGDGSGARIHLMSGQ"
     gene            3143..3922
                     /locus_tag="PKV97_12930"
     CDS             3143..3922
                     /locus_tag="PKV97_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HNB06829.1"
                     /translation="MSAATHRPMPAPARPCAAAAAVIECNAAGAGRGLLPLVRLLQLA
                     SPALPVGAYTYSQGLEWAVESGLVRSEAEAAAWIGELLEWSLARFEVPLLGCQLAAWS
                     RNEDAELARLNDDFLASRETAELRAETVQMGWSLVRLLAELDAFASIPGWRARLLAIE
                     APAFPTAWSAAAAAWQVAPGEALVAYLWAWLENQVMAAVKAVPLGQSAGQRLLATLGD
                     RIPALAAAALVLPETAWSNYTPGLAIASSRHETQYTRLFRS"
     gene            3919..>4231
                     /locus_tag="PKV97_12935"
     CDS             3919..>4231
                     /locus_tag="PKV97_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNB06830.1"
                     /translation="MTPISALHAAHAPTTARAHAGPQRGPLRVGIGGPVGSGKTALTL
                     AMCLALRDKYDIAVVTNDIYTAEDAQFLVRNEALAPERIIGVETGGCPHTAIREDASI
                     NL"
BASE COUNT          657 a         1538 c         1491 g          545 t
ORIGIN      
        1 ggcctgcgag cccggccggc agtggttcac ccgcctgtgg gcgacggcgc gcccggcagt
       61 cgcgggacgg gcggcgcaca cgccgcggat ctggaacacc tgaaacccat gggcggcagc
      121 gcgccgccgc ggaggaagac gatggaactg agcccacgcg agaaggacaa gctgctggtg
      181 ttcaccgccg gcctgctcgc cgaacgccgg cgcgcgcgcg gcctgaagct gaactacccg
      241 gaggcggtgg cgctgatcac ctgcgcgatc ctcgagggcg cgcgcgacgg ccgcacggtg
      301 gcggagctga tgggctacgg cgcgaccctg ctgacgcgcg cggacgtgat ggcgggcgtc
      361 gcggagatga tccccgacat ccaggtggag gcgacgtttc ccgacggcac caagctggtg
      421 accgtgcaca acccgatcgt gtgaggagca ggccgatgat ccccggagaa ctcttcgtcg
      481 ccgacggcga catcgaactc gccgccggcc gtgccacgcg cacgctggtg gtgagcaaca
      541 ccggcgaccg cccgatccag gtcggctcgc actaccactt cgccgagacc aacagcgcgc
      601 tcgacttcga ccgcgcggcg gcgcgcggct tccgcctgaa catcgccgcc ggcacggcgg
      661 tgcgcttcga gcccggacag tggcgcaccg tcgagctggt ggcgctcgat ggcgagcgcg
      721 aggtgtacgg cttcaacggc ctcgtcatgg gacggctgga cgagcccgcg accgcgcccg
      781 ccgcgcaccg gacgcaaggg agcctgtgat ggcgacgatc tcgcgccgcg cgtacgcgga
      841 gatgttcggc cccacggtgg gcgaccggct tcgcctggcc gacaccgagc tggtgatcga
      901 ggtggagaag gacttcacca tctacggcga agaggtgaag ttcggcggcg gcaaggtgat
      961 ccgcgacggc atgggccagg gccagggcgt ctcggccgac gtggcggaca ccatcgtcac
     1021 caacgcgctg atcatcgacg cggtggccgg catcgtgaag gccgacgtcg gtctcaagga
     1081 cggccgcatc tggaggatcg gcaagggcgg caacccggac atccagcccg gggtgacgat
     1141 cccgatcggc gccggcaccg aggtgatcgc cggcgagggc atgatcctca ccgcgggcgg
     1201 catcgacagc cacatccact ggatctgccc gcagcagatc gaggaggcgc tgagctcggg
     1261 cgtcaccacc atgctgggcg gtggcaccgg gccggcgacc ggcaccttcg cgaccacgtg
     1321 cacgccgggc ccctggcaca tccaccgcat gctggaggcg gccgaggcct tcccgatgaa
     1381 cctcggcttc ttcggcaagg gcaacgccag cctgccggcg ccgctgaagg agcaggtgca
     1441 ggccggcgtg atcgggctga agctgcacga ggactggggc acgacgccgg cggcgatcga
     1501 caactgcctg acggtggccg agcagatgga catccaggtc gccatccaca ccgacacgct
     1561 caacgagtcg ggtttcgtcg agaccacgct ggcggcgttc aaggatcgca ccatccacac
     1621 cttccacacc gagggcgcgg gcggcggcca cgcgccggac atcatccgcg cgatcagccg
     1681 ccccaacgtg ctgccgtcct cgaccaaccc gacgcggccc tacacggtca acaccatcga
     1741 cgagcacctc gacatgctga tggtgtgcca ccacctcgat ccgagcatcg ccgaggacgt
     1801 ggccttcgcc gaatcgcgca tccgccgcga gaccatcgcc gccgaggaca tcctgcacga
     1861 caccggcgcg ttctcgatga tgtcgtccga ctcgcaggcc atgggccggg tgggcgaggt
     1921 ggtgatccgc acctggcaga ccgcgcacaa gatgaagctg cagcgcggct ggctggcgcc
     1981 gcggcgcagc gcggcggcgg cggtgccgga cgcggtggcc gtggtcgagg gcagcaccgc
     2041 caacgacaac ttccgcgtca agcgctacat cgccaagtac accatcaatc ccgcgctgac
     2101 ccacggcatc gcccacgagg tgggctcgat cgagccgggc aagctcgccg acctggtgct
     2161 gtggcgcccg gccttcttcg gcgtcaagcc gagcctggtg atcaagggcg gcatgatcgc
     2221 cgcggccgcg atgggcgacc ccaacgcctc gatcccgacc ccgcagccgg tgcattggcg
     2281 gccgatgttc ggcagcttcg gacgcgcgct gaaatgcgcg gtgaccttcg tgtcgcaggc
     2341 cgcgctgcac aacgccgcgg tggccgcgct cgggctgcag aagccgctgg tggcggtgaa
     2401 gggctgccgc acgctcacga aggccgacat ggtgctcaac gacgccacgc cgcagatcga
     2461 ggtcgatccc gagacctatg tcgtgcgcgc cgacggcgag cacctcgcct gcgaacccgc
     2521 caccgagctg ccgctggcgc aacgctattt cctgttctga gcgcagcgca ccggaaccgc
     2581 actggagacc tcccgccatg ccgatgaccg cagaaaccct ggccgacttc gtgatgcccg
     2641 ctcccgccac cgacgtgctg ctgatcgagc agttccacga gggcgacgag ccagcgagcg
     2701 aggtgctcga gctcgacttc ggcgcccgca ccaagagccg cctgcgcacc cggctcgcct
     2761 ccggcgccga ggtgggcctc ttcctgccgc gcggcaccat cctgcgcggc ggacagaagc
     2821 tgcgcgcacg cgacggccgc atcgtcgagg tgcgcgccgc tgccgaggac ctgctcgagg
     2881 cgcgctgcgc ctcggcggta gcgctggcgc gcgccgcctt ccacctcggc aaccgccacg
     2941 tcgcggtcga ggtcgggcac gacgaggccg gcgactggct gcgcctccag gccgatcacg
     3001 tgctcgaagg catgctggtg gggctgggcg cactggtgag cgcgctgcgc gcgcccttcg
     3061 agcccgaggc cggcgcctat gcgcccggcc accagcaccc gggcgacggc agcggcgcgc
     3121 gcatccacct catgagcggg caatgagcgc cgccacccat cgcccgatgc cggcgccggc
     3181 aaggccatgc gccgccgcag ccgctgtcat cgaatgcaac gccgcgggag cgggtcgcgg
     3241 cctgttgccg ctggtgcgcc tgctgcagct cgccagcccg gcactgcccg tcggcgccta
     3301 cacctactcg caaggcctgg agtgggcggt ggagagcggc ctcgtgcgca gcgaggcgga
     3361 ggccgcggcg tggatcggcg agctgctgga atggagcctg gcacgcttcg aggtgccgct
     3421 gctcggttgc cagctcgcgg cgtggtcgcg aaacgaagac gccgagcttg cgcggctcaa
     3481 tgacgacttt ctcgccagcc gcgagactgc cgagctgcgc gccgagacgg tgcagatggg
     3541 ctggtcgctg gtgaggctgc tggccgagct ggatgcgttt gcgtcgattc ccggctggcg
     3601 cgcgcgcctg ctggcgatcg aggcccccgc ctttcccacc gcgtggtcgg ccgccgccgc
     3661 ggcctggcag gtagcgcccg gcgaggcgct cgtcgcctac ctgtgggcct ggctggagaa
     3721 ccaggtgatg gccgcggtga aggccgtgcc gctcggccag agtgccgggc agcgcctgct
     3781 cgccacgctc ggcgaccgca tcccggcgct ggcggcggcg gcgctcgtgc tgcccgagac
     3841 ggcctggagc aactacaccc ccggcctggc gatcgccagc agccggcacg agacccaata
     3901 cacgaggctg ttccgatcat gaccccgatt tctgccctcc acgccgcgca tgcccccacg
     3961 accgcccgtg cacacgccgg accgcagcgc ggccccctgc gcgtcggcat cggcggcccg
     4021 gtcggttcgg gcaagaccgc gctgaccctg gcgatgtgcc tggccctgcg cgacaagtac
     4081 gacatcgccg tggtcaccaa cgacatctac accgccgagg acgcccagtt cctcgtgcgc
     4141 aacgaggcgc tcgcgcccga gcgcatcatc ggcgtggaga ccggcggctg cccgcacacc
     4201 gcgatccgcg aggacgcctc gatcaacctc g
//
LOCUS       DAOQNN010000281         4199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13451_length_4199_cov_8.727558, whole genome shotgun sequence.
ACCESSION   DAOQNN010000281 DAOQNN010000000
VERSION     DAOQNN010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13451_length_4199_cov_8.727558"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PKV97_12940"
     CDS             <1..236
                     /locus_tag="PKV97_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNB06831.1"
                     /translation="ARREPAREREGLRAGPRRGRSSVAADGFDFSKPYEPLNPPVAVA
                     EQTQPTVAAAGKPDPLRRHQRPIAVLLGGLGRK"
     gene            361..2163
                     /locus_tag="PKV97_12945"
     CDS             361..2163
                     /locus_tag="PKV97_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB06832.1"
                     /translation="MNPSGHLPGLPPSPGPSSAEPFVADLSEGAETGVYLALLELLDE
                     GLLITGDEVVLDANSAACRLLGRDYRDVAGRPLADLFPDGQAFLAARARLLIEGERRG
                     RLDFALPDGGVRALEFNCAPRLRPGIHAIILSAPAVVAEYETGTPVLAAPRPTAGGAR
                     AFDDAPSMSMYLTSHDPLTGLANRRMLEARFGDCAVRAAGRHGSIAIVRLDLDGFAAV
                     NRSLGESAGDEVLRCIARRLAATAGSAALVARERSDSFIVLLPEPAHPGNADQFAETL
                     LRAVAEPLELGGGLQRITASAGLALYPQDGRNLDTLLRNAKSALEQARRLGGNHCSVF
                     HTRLDGPGFDSSHLERGLRHALQADELCLHFQPLVDARSGRTRAGEALLRWRHPELGL
                     LPYRQFMGAVGDPRLLADVGDWVLATACDHATDWPPTHDGECLRLTVNVSVEQLMRGD
                     FAARVATALRTSGLKAERLELDLDEKVLALDSPTLAATLERIAATGVRLSIDDFGRGL
                     SSIPRLRRYPLAALKLDPELVRGVGRSEDSEAIVEAVGALAGTLGLEIYARGVEHRGQ
                     QAFLCALDCHLQQGPLFGRPLAAREFADRLLRAQ"
     gene            complement(2157..2468)
                     /locus_tag="PKV97_12950"
     CDS             complement(2157..2468)
                     /locus_tag="PKV97_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06833.1"
                     /translation="MRVCLFAFLLVVPLGLSSAPALAQKAPAAPAANPPTPEWRRTDG
                     YRFPALADSVRRLQYTTLAMQLREYCADRRVSDDFVRERLQRFGRITGRAETCASLRD
                     Y"
     gene            complement(2567..3946)
                     /locus_tag="PKV97_12955"
     CDS             complement(2567..3946)
                     /locus_tag="PKV97_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNB06834.1"
                     /translation="MTSNWFVNMAYFIAAIVFIFGLKAMASPVTARKGVVWAGYAMIA
                     ATLATFFIPDLHNIGLMVVAIVLGGGVAWYSGKVVKMTDMPQMVAIYNGMGGGAAAGI
                     AAIELVRGVPHDAITSTLLALGAVIGAVAFSGSCVAFAKLQGLMTKAIRLPGQNNINF
                     LLSVITIGIGLTVAYSAHPGGFEIFLFFVLALALGVILVMPIGGADMPVVISLLNAFT
                     GLAVGFKGFVIGNPALIVAGIVVGASGMLLTQLMAKAMNRPIKNVIFAPITGAAVEGG
                     EAVEGTMRELSAMDAAALMRYGQKVIIVPGYGMAVAGAQHKVWEMAQLLEEGGVEVVF
                     AIHPVAGRMPGHMNVLLAEAGVPYDKIFDLDEINNDFAQADVALVIGANDVVNPVART
                     DKSSPIYGMPILNADMAQNVIVVKRGKGAGYSGIENALFYKDNCRMLYGSAQEAIAEV
                     IQHVKSMEA"
     gene            complement(3943..>4199)
                     /locus_tag="PKV97_12960"
     CDS             complement(3943..>4199)
                     /locus_tag="PKV97_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNB06835.1"
                     /translation="YEVISRVPVILHTPLMSGSNFVHGVVLIGSMVALGHADPESPLQ
                     LLIGFFAVFLGAANAAGGYIVTERMLGMFKAGGRKQGGEQ"
BASE COUNT          626 a         1571 c         1402 g          600 t
ORIGIN      
        1 aggctcgtcg cgagccagcg cgcgagcgcg aaggcctgcg cgccggcccc cgccgtggcc
       61 gcagcagcgt cgccgccgac ggcttcgact tctccaagcc ctacgagccg ctcaatcccc
      121 ccgtcgcggt cgccgagcag actcagccca ccgtggccgc tgccggcaag cccgacccgc
      181 tacgtcgcca ccagcgtccg atcgcggtgc tgctcggcgg cctcgggcgc aaataagggg
      241 caccccggcc ctttattcgc cacacggcgg cgcacaggca tcggctatgc tgtggtctca
      301 ctcgcaggct cccccgcccg ggggcgcccg catcgaacac ccctgcgatc ctctgctgtc
      361 gtgaaccctt ccggacacct gccgggcctg ccgccctccc ccggcccctc ctctgccgag
      421 cccttcgtcg cggacctttc ggagggcgcc gagaccggcg tatacctcgc cctgctcgaa
      481 ctgctcgacg agggcctgct gatcaccggc gacgaggtcg tgctcgacgc caacagcgcc
      541 gcctgccgcc tgctcggccg cgactaccgc gacgtggcag ggcgtccgct cgccgacctc
      601 ttccccgacg gccaggcctt cctggccgcg cgcgcgcgcc tgctgatcga gggcgaacgc
      661 cgcggccgcc tcgacttcgc tctcccggat ggcggcgtgc gcgcgctcga gttcaactgc
      721 gcgccgcggc tgcgccccgg catccacgcc atcatcctga gcgcgcccgc ggtcgtggcg
      781 gagtacgaaa cgggcacccc cgtcctcgca gcgccacgcc cgacagcagg cggtgcgcgc
      841 gcgttcgacg acgcgccgtc gatgtcgatg tacctgacga gccacgaccc gctcaccggc
      901 ctggccaacc gccgcatgct cgaggcgcgc ttcggtgact gcgcggtgcg cgcggcgggc
      961 cgccacggca gcatcgcgat cgtgcgcctc gacctcgacg gcttcgccgc ggtcaatcgc
     1021 agtctgggcg agagcgccgg cgacgaggtc ctccggtgca tcgcccgccg cctcgccgcc
     1081 accgccggtt ccgctgccct ggtggcgcgc gagcgcagcg acagcttcat cgtgctgctg
     1141 cccgagccag cgcatcccgg caacgccgac cagttcgccg aaaccctgct gcgcgcagtg
     1201 gccgagcccc tcgagctcgg cggcgggctg cagcgcatca ccgccagcgc cggcctcgcc
     1261 ctctatccgc aggacgggcg caacctcgac accctgctgc gcaacgccaa gagcgcgctc
     1321 gaacaggcac gccggctcgg cggcaaccac tgcagcgtct tccacacccg gctcgacggc
     1381 cccggcttcg acagcagcca tctcgaacgc ggcctgcgcc acgccttgca agccgacgag
     1441 ctgtgcctgc acttccagcc gctggtcgat gcgcgctccg gtcgcacccg tgcgggcgag
     1501 gccttgctgc gctggcgcca ccccgagctc ggcctgctgc cctaccgcca gttcatgggt
     1561 gcggtcggcg acccccgcct gctcgccgac gtcggcgact gggtgctggc cactgcctgc
     1621 gaccacgcca ccgactggcc gccgacgcac gatggcgaat gcctgcgcct gacggtgaat
     1681 gtctcggtcg agcagctcat gcgcggcgac ttcgcggcgc gcgtcgcgac cgccctgcgc
     1741 acctccgggc tgaaggcgga gcggctcgag ctcgacctcg acgaaaaggt gctggcactc
     1801 gacagcccga cgctcgccgc cacgctggag cgcatcgccg ccaccggcgt gcgcctgtcg
     1861 atcgacgact tcggccgcgg cctgtcgtcc atcccgcgcc tgcgccgcta cccgctcgcc
     1921 gcgctcaagc tcgaccccga gctggtacgc ggggtcggtc gcagcgagga cagcgaggcc
     1981 atcgtcgagg cggtcggcgc gctcgccggc acgctcgggc tggaaatcta cgcgcgcggg
     2041 gtcgagcacc gcggccagca ggccttcctg tgcgcgctcg actgccacct gcagcaaggg
     2101 ccgctgttcg ggcggccgct ggcggcgcgg gagttcgcgg accgcctgct ccgcgctcag
     2161 taatcccgca ggctggcgca ggtctccgcg cgcccggtaa tacgaccgaa gcgctgcagg
     2221 cgctcgcgca cgaagtcgtc ggacacgcgc cggtcggcac agtattcgcg cagctgcatg
     2281 gccagcgtgg tgtattgcag gcggcgcacg ctgtcggcga gcgccgggaa acgatagccg
     2341 tcggtgcgcc gccactcggg cgtcggcgga ttcgctgcag gcgcagcggg ggccttctgc
     2401 gcaagggccg gagcgctgga aaggccgagc gggacgacaa ggaggaaagc gaaaaggcaa
     2461 acgcgcatcg ggccgcaggt acaggaaggt tccctgccct tacggcccga tgcgcctccg
     2521 gcttgagcgg aacccgccgg cgctgcagcc ggcgggcggt tcggcgtcat gcctccatcg
     2581 acttcacgtg ctggatgacc tcggcgatgg cttcctgcgc gctgccgtag agcatgcggc
     2641 agttgtcctt gtagaacagc gcgttctcga tgcccgagta gccggcaccc ttgccgcgct
     2701 tgaccacgat gacgttctgc gccatgtcgg cgttgaggat cggcatgccg tagatcgggc
     2761 tcgacttgtc ggtgcgcgcg accgggttca ccacgtcgtt ggcaccgatc accagggcca
     2821 cgtcggcctg ggcgaagtcg ttgttgatct cgtcgagatc gaagatcttg tcgtagggca
     2881 cgccggcctc ggcgagcagc acgttcatgt ggccgggcat gcggcccgcg accggatgga
     2941 tcgcgaacac cacctcgacg ccgccctcct ccagcagctg cgccatctcc cacaccttgt
     3001 gctgggcgcc ggccaccgcc atgccgtagc ccggcacgat gatgaccttc tggccatagc
     3061 gcatcagcgc cgccgcatcc atcgccgaga gctcgcgcat cgtgccttcg accgcctcgc
     3121 cgccttccac ggctgcgccg gtgatcgggg cgaagatgac gttcttgatc gggcggttca
     3181 tcgccttggc catgagctgg gtgagcagca tgcccgaggc gccgaccacg atgccggcca
     3241 cgatcagcgc cgggttgccg atcacgaagc ccttgaagcc gaccgcgagg ccggtgaagg
     3301 cgttcagcag cgagatcacc accggcatgt cggcgccgcc gatcggcatc accaggatca
     3361 cgccgagcgc gagcgcgagc acgaagaaca ggaagatctc gaagccgccc gggtgcgccg
     3421 agtaggccac ggtgaggccg atgccgatgg tgatgaccga gagcaggaag ttgatgttgt
     3481 tctgccccgg caggcggatc gccttggtca tcagcccctg cagcttggcg aaggccacgc
     3541 acgaacccga gaacgccacc gcgccgatca ccgcgccgag cgcgagcagc gtggaggtga
     3601 tcgcgtcgtg cggcacgccg cgcaccagct cgatcgccgc gatgccggcc gcggcgccgc
     3661 cgcccatgcc gttgtagatc gcgaccatct gcggcatgtc ggtcatcttg accaccttgc
     3721 cgctgtacca ggcgacgccg ccgccgagca cgatcgccac caccatcagg ccgatgttgt
     3781 gcaggtcggg gatgaagaag gtggccaggg tcgcggcaat catcgcgtag ccggcccaca
     3841 ccacgccctt gcgcgcggtg accggcgagg ccatcgcctt gaggccgaag atgaacacga
     3901 tggcggcgat gaagtacgcc atgttgacga accagttcga ggtcattgct cacccccctg
     3961 cttgcggccg ccggccttga acatgcccag catgcgctcg gtgacgatgt agccgccggc
     4021 ggcgttggcc gcgccgagga acaccgcgaa gaagccgatc agcagctgca gcgggctttc
     4081 cgggtcggca tggccgagcg ccaccatgga tccgatcagc acgacgccat gcacgaagtt
     4141 ggagcccgac atcagcgggg tgtgcaggat caccggcacg cgcgagatca cctcgtaac
//
LOCUS       DAOQNN010000282         4196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13468_length_4196_cov_9.931901, whole genome shotgun sequence.
ACCESSION   DAOQNN010000282 DAOQNN010000000
VERSION     DAOQNN010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13468_length_4196_cov_9.931901"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKV97_12965"
     CDS             complement(<1..406)
                     /locus_tag="PKV97_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNB06836.1"
                     /translation="MSQTVEVKVPDIGDFKDVPVIEILVEPGDMVKAEDPLVTLESDK
                     ATMDVPSPTAGRVKELRVKLGDKVSEGALVLVLEADAAEKTATPASQPTRAQAPASTT
                     LPAASPATASTCSYKGEVDLDTDLLVLGAGPGG"
     gene            complement(403..981)
                     /locus_tag="PKV97_12970"
     CDS             complement(403..981)
                     /locus_tag="PKV97_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06837.1"
                     /translation="MTTITVVKKNGHIAISAESLTTFGDTRLGAEYKAEHDKILHIGD
                     SWIGVCGSSAHHLVLAHLLAKIEDLRLGSRSEVFETFRKLHPMLKEQAFLNPKEDEDD
                     PYESSQITALIANPTGIYGVYSYREVFAYDRFWAVGSGRSFALGAMYAAYERTKTAAE
                     IAAIGVAAGVEFDTASSGPIVTHAVKLIKEKK"
     gene            complement(1046..2647)
                     /gene="aceF"
                     /locus_tag="PKV97_12975"
     CDS             complement(1046..2647)
                     /gene="aceF"
                     /locus_tag="PKV97_12975"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HNB06838.1"
                     /translation="MSQIIEVKVPDIGDYKDVPVIEVMVKPGDVVKAEDALVTLESDK
                     ATMDVPAPAAGTVKELRLKVGDKVSEGTLVLVLEAGTAEKPATPASQPAPAQTPATAP
                     AAATSVIEVKVPDIGDYKDVPVIEVMVKVGDAVKAEDPLVTLESDKATMDVPAPAAGV
                     VQALRVQLGDKVSEGSVILTLAAAGTAVTAMAAAAAPAPSQASVSPAPAAPSTSPAPV
                     PAATAPAGKAHASPSVRKFARELGVDVSKVAGSGPKGRILHDDVQRFVKGVMAQVASG
                     TAPTASSGTSLGGGLDLLPWPKVDFAKFGEIEARPLSRIKKISGQNLSRNWVMIPAVT
                     YHEDADITDLEAFRIQVNKENEKSGAKLTMLAFLIKACVKALQKFPEFNASIDGDNLV
                     YKKYFHIAFAADTPNGLVVPVVKNADQKGVFEIAAETGELAKKARDGKLAPTEMQGAC
                     FTISSLGGIGGTYFAPIVNAPEVAILGVNKSAMKPVWDGKEFVPRLMLPLSLTADHRV
                     IDGALATRFNVYVAQLLADIRRMML"
     gene            complement(2664..>4196)
                     /gene="aceE"
                     /locus_tag="PKV97_12980"
     CDS             complement(2664..>4196)
                     /gene="aceE"
                     /locus_tag="PKV97_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936832.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNB06839.1"
                     /translation="HKGEPTVILAKTIKGYGMGSSGEAANIAHQAKKMSRDSLMKFRD
                     RFKLPLSDEQVDNLVFLKFPDGSKELEYMRARRMDLGGYLPARRRKADALAVPPLSAF
                     EPLLKASGEGREVSTTMAFVRILNILLKDKQVGKRIVPIVPDESRTFGMEGMFRQIGI
                     WNQQGQKYLPQDHDQLMFYKESKDGQILQEGINEAGAMADWIAAATAYSTHGLQMIPV
                     YIFYSMFGLQRTGDLCWAAGDMRARGFLVGGTAGRTTLNGEGLQHEDGHSHLWSGAIP
                     NCVSYDPTFSYELAVIVQDGLRRMYAEQEDIYYYLTVMNENYEHPEMPAGAEADILKG
                     MYSFRQGAASNGPRVQLLGSGTIFREVIAAAEMLKKDWGVESDLWGCPSFTELAREGH
                     AAARWNLLHPTEKQRPSHVETCLKDTRGPVIAATDYVKLFADQIRAFVPRRYVVLGTD
                     GFGRSDTREKLRHFFEVDRNWVTVAALKALADDGQIDRSKVAEAISKYGLDVNKPNPM
                     TV"
BASE COUNT          658 a         1328 c         1359 g          851 t
ORIGIN      
        1 agccgccagg gcctgctccc agcaccagca ggtcggtgtc gaggtcgact tcacccttgt
       61 agctgcaggt tgaagctgtt gcgggcgatg ccgctgggag ggtcgtggat gctggcgctt
      121 gcgctcgtgt cggctgcgag gccggtgtgg cggtcttctc agcggcatcg gcctcgagaa
      181 ccaataccag cgcgccttcc gagaccttgt cgccaagctt cacgcgcaat tccttcaccc
      241 tgccggccgt gggcgagggt acgtccatcg tcgccttgtc ggattcgagg gtgaccaagg
      301 gatcttcggc ctttaccata tcgccgggtt cgacgaggat ttcgatcacc ggcacatcct
      361 tgaaatcgcc gatgtcgggc accttcactt cgactgtctg gctcatttct tttccttgat
      421 cagtttgacg gcgtgggtca cgatcgggcc gctcgatgca gtgtcgaatt ccacgcccgc
      481 ggcaacgccg attgcggcga tctcagccgc ggtcttggtc cgctcatagg cggcgtacat
      541 cgcgccgagg gcaaaactgc ggcccgagcc gacagcccag aagcggtcgt aggcgaacac
      601 ctcgcggtac gaatacacgc cgtagatgcc ggtgggattg gcgatcagcg cggtgatctg
      661 cgacgactcg tagggatcgt cctcatcctc tttcggattg aggaaggcct gctccttgag
      721 catcggatgc agtttgcgaa aggtttcgaa cacttcgctg cgcgaaccga gccggagatc
      781 ttcgatcttc gccagcagat gcgccagcac cagatgatgc gccgaactgc cgcagacgcc
      841 gatccacgaa tcgccgatgt gcaggatctt gtcgtgctcg gccttgtatt cggcgccgag
      901 ccgcgtatcg ccgaaggtgg tgagcgattc agccgaaatt gcgatgtggc cgttcttctt
      961 gacgacggtg atggtggtca tggcaatggc ggagtgagtt cgggagcagc gattatcgcc
     1021 tgcgcccgaa tcctctccgg tttcctcaga gcatcatccg ccggatgtcg gcgagcaact
     1081 gcgcgacata gacgttgaag cgcgtcgcca gcgcgccgtc gatcacgcgg tgatcggcgg
     1141 tcagcgacaa cggcagcatc agccgtggca cgaactcctt gccatcccag acaggcttca
     1201 tcgcgctctt gttcacgccg aggatcgcca cttccggcgc gttgacgatc ggggcgaagt
     1261 aggtgccgcc gatgccgccg aggctggaaa tcgtgaagca tgcaccctgc atctcggtcg
     1321 gcgcgagctt gccgtcgcgc gccttcttcg ccagttcgcc ggtttcggcg gcaatctcga
     1381 acacgccctt ttgatccgca ttcttcacca ctggcaccac cagcccgttg ggcgtgtcgg
     1441 ccgcgaaggc gatgtggaag tacttcttgt aaacgaggtt atcaccgtcg atgctggcgt
     1501 tgaactcggg gaacttctgc aacgccttga cgcaggcctt gatcaggaaa gcgagcatgg
     1561 tcagtttggc gcccgacttt tcgttctcct tgttcacctg gatgcggaag gcttcgaggt
     1621 cggtgatgtc ggcatcctcg tgataggtga cagccgggat catcacccag ttgcgcgaca
     1681 ggttctggcc ggaaatcttc ttgatgcgcg acagtggcct ggcctcgatc tcgccaaact
     1741 tggcgaaatc caccttcggc catggcagca ggtcgaggcc gccgccgagc gaggtgccgc
     1801 tggatgctgt cggtgcggta ccgctggcca cctgcgccat gacgcccttg acgaagcgct
     1861 gcacatcgtc gtgcaggata cggcctttcg gcccactgcc agcgaccttc gagacatcga
     1921 cgccgagttc gcgcgcgaac ttgcgcaccg aggggctggc gtgcgccttg ccggcgggcg
     1981 cggtggcagc cgggacgggt gcgggcgacg tgctcggcgc agccggcgcc ggagacaccg
     2041 atgcctgcga cggcgcaggg gcggctgcag cggccattgc ggtgaccgcc gtacctgcgg
     2101 ccgcgagcgt aaggatcacg ctgccttccg aaaccttgtc gccgagctgc acgcgcagtg
     2161 cctgcaccac gccggcggcg ggggcgggca cgtccatcgt tgccttgtcg gattcgagcg
     2221 tgaccagggg gtcttccgcc ttcacggcat cgccgacctt gaccatgacc tcgatcaccg
     2281 gcacgtcctt gtagtcgccg atatcgggca ccttcacttc tatgacgctc gtggctgcag
     2341 ccggtgctgt tgctggtgtt tgggcgggtg caggctgcga ggcaggcgtg gcgggcttct
     2401 ctgcggtgcc tgcctcgaga accaatacca gcgtgccttc cgagaccttg tcgccgacct
     2461 tgaggcgcag ttccttcacc gtgccggcag ccggcgcggg tacgtccatg gtcgccttgt
     2521 ccgattcgag cgtgaccagc gcatcttccg ccttcaccac gtcgcccggc ttgaccatca
     2581 cctcgatgac cggcacgtcc ttgtagtcgc cgatgtcggg aaccttgact tcgattatct
     2641 ggctcattgt cgtcgtcccg ttttcagacc gtcatcggat tcggtttgtt cacgtcgagg
     2701 ccgtatttcg agatcgcttc ggccaccttg ctgcgatcga tctggccgtc gtcggccagc
     2761 gccttgagtg cggcgaccgt gacccagttg cggtcgacct cgaagaagtg gcgcagcttc
     2821 tcgcgtgtgt cggagcggcc gaagccgtcg gtgcccagta ccacgtagcg gcgcggcacg
     2881 aaggcacgga tctggtctgc gaacagcttg acgtagtcgg tcgcggcaat caccgggccg
     2941 cgcgtgtcct tgaggcaggt ttcgacgtgc gacggcctct gcttctcggt cggatgcagc
     3001 aggttccagc gtgcggcggc atggccttcg cgcgccagtt cggtgaaact cgggcagccc
     3061 cacagatcgg attcgacgcc ccagtccttc ttcagcatct cggcggcggc gatcacctcg
     3121 cggaagatcg tgccgctgcc cagcaactgc acgcgcgggc cattgctggc cgcgccctgg
     3181 cggaacgagt acatgccctt gagaatgtcg gcctcggcgc cggccggcat ctcgggatgt
     3241 tcgtaattct cgttcatcac cgtcaggtag tagtagatgt cctcctgttc ggcatacatg
     3301 cggcgcaggc cgtcctgcac gatgaccgcg agttcgtagg agaaagtcgg gtcgtagctg
     3361 acgcagttcg ggatcgcgcc cgaccacagg tggctgtggc cgtcctcgtg ctgcaggcct
     3421 tcgccgttga gcgtggtgcg cccggcggtg ccgccgacca ggaagccgcg cgcgcgcatg
     3481 tcgcctgccg cccagcacag gtcgccggtg cgctgcaggc cgaacatcga atagaagatg
     3541 tagaccggaa tcatctgcag gccgtgcgtc gagtaggccg tcgccgcggc gatccagtcg
     3601 gccatcgcgc cggcctcgtt gatgccctcc tgcaggatct ggccgtcctt cgattccttg
     3661 tagaacatca gctggtcgtg gtcctgcggc agatacttct gtccttgctg gttccatatg
     3721 ccgatctggc ggaacatgcc ctccatgccg aaggtgcgcg actcgtccgg cacgatcggc
     3781 acgatgcgct tgccgacctg cttgtccttg agcaggatgt tgaggatgcg cacgaaggcc
     3841 atggtggtgg acacttcgcg gccctcgccg ctcgccttga gcagcggttc gaaggccgac
     3901 agcggcggca ccgcgagtgc atcggccttg cggcggcgcg ccggcaggta gccgccgaga
     3961 tccatgcgcc gcgcgcgcat gtactcgagt tccttcgagc cgtcggggaa cttgaggaag
     4021 accagattat cgacctgctc gtcggagagc ggcagcttga agcggtcgcg gaacttcatc
     4081 agcgagtcgc gcgacatctt tttcgcctgg tgggcgatgt tcgccgcttc gcccgaggaa
     4141 cccatgccgt agcccttgat cgtcttggcg aggatcaccg tgggttcacc cttgtg
//
LOCUS       DAOQNN010000283         4195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13473_length_4195_cov_10.305556, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000283 DAOQNN010000000
VERSION     DAOQNN010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13473_length_4195_cov_10.305556"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..720)
                     /gene="plsY"
                     /locus_tag="PKV97_12985"
     CDS             complement(112..720)
                     /gene="plsY"
                     /locus_tag="PKV97_12985"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNB06840.1"
                     /translation="MSTLLLIVAAYLLGSIPFAIVTSRLFGLEDPRKYGSGNPGATNV
                     LRSGNKAAAVLTLLGDALKGWLAVWAALRLGFEPDIAALAGLAAFLGHVFSLFLRFNG
                     GKGVATALGVLAGVDARIAIFCAVIWLMVAYTTRYSSAAALSAALAAPLAGLLFLGAK
                     PAVGLLAVMTAVLFWRHKENIARLRAGTEGKIGGKKKDGPAA"
     gene            888..1262
                     /locus_tag="PKV97_12990"
     CDS             888..1262
                     /locus_tag="PKV97_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNB06841.1"
                     /translation="MDFIFIEELRVKAWVGIYAREKTGPQTVELNLTFGVPDAAAEHD
                     DIADTIDYAVVTERIRRELGERHFNLIESLGEFVVKLLFEEFGAPWVKLKVGKIGVMK
                     DVRRVGVYIQRSKAGVVVPPAA"
     gene            complement(1282..1770)
                     /gene="ybaK"
                     /locus_tag="PKV97_12995"
     CDS             complement(1282..1770)
                     /gene="ybaK"
                     /locus_tag="PKV97_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNB06842.1"
                     /translation="MSRNEAHAPETPATRFLRQHGVAFSSHLYEYEEHGGTTVSSREL
                     NVPEHAVVKTLIMEDENAQPMVVLMHGDHKVSTKELARQIPCKHVETCKPETANRHSG
                     YLVGGTSPFGTRKKMPVYMERTVLDLPLVYINGGRRGFLVGIHPHDLLRVLQPRLVQV
                     AN"
     gene            complement(1886..2815)
                     /gene="xerD"
                     /locus_tag="PKV97_13000"
     CDS             complement(1886..2815)
                     /gene="xerD"
                     /locus_tag="PKV97_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNB06843.1"
                     /translation="MSGRAPAREAGLQPAHRAELDRFADALWLEHGLARNTLAGYRSD
                     LARFAAWLETRAQRLPAAAGADLAAYLAEFSRAAKPASQRRLIAAWRRYYRLLLANRE
                     IAEDPTLAIDTPRAAERFPKTLSEPQVEALLAAPALDTPQGLRDRCMLEVLYAAGLRV
                     SELVGLKIFAVGVNEGVVRVMGKGSKERLVPLGEIAADWLQRYLAEARPVLLAGRSCD
                     EIFVTRLGAAMSRQMFWRLIKHYALAAGIAPERISPHTLRHAFATHLLNHGADLRVVQ
                     LLLGHADISTTQIYTHVARERLKQLHARHHPRA"
     gene            complement(2812..3318)
                     /locus_tag="PKV97_13005"
     CDS             complement(2812..3318)
                     /locus_tag="PKV97_13005"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNB06844.1"
                     /translation="MSRAPVTTTGGADTSAFDAIIALPFGPFGIRAHDGQVQELVFLP
                     PGTPLHEPRNASAGAAAHALRAWIDDPDQPFPVPLAPCGTPFRRRVWTAIAAIPRGRT
                     ATYGELAASLDSAPRAVGQACGANPFPLIVPCHRVTSAAGLGGFANAREGWLLDVKRW
                     LLAFEGAL"
     gene            complement(3315..3899)
                     /locus_tag="PKV97_13010"
     CDS             complement(3315..3899)
                     /locus_tag="PKV97_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNB06845.1"
                     /translation="MSIRILRSLIVAALLSASLAQAAVKVGNVEFAPQQTVQQRTLEL
                     NGAGLRTRLGFKVYAMGLYLRAPLAEAGAILADKGEKRIRIVPIRDLEAKQFGDALQT
                     GLEKNHDAAALAALKPATDALLGALAAVGEVKAGTEIVLDRLGNGATRLLVDTKQQGE
                     DIADAAFYPALLRIWLGERPADSELREALLKTPQ"
     gene            complement(3935..>4195)
                     /locus_tag="PKV97_13015"
     CDS             complement(3935..>4195)
                     /locus_tag="PKV97_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HNB06846.1"
                     /translation="HINTCSSGHTEAIVTENYSHAMRFLREVDSSSVMVNASTRFADG
                     FEYGLGAEIGISTDKIHARGPVGLEGLTSQKWIVFGNGEVRR"
BASE COUNT          589 a         1369 c         1611 g          626 t
ORIGIN      
        1 tgtaggagcg gcgcaagccg cgaacccggc gcccggcatc agagccgccg ctcctccccc
       61 gcattcgcgc ctgcgggcgc tcctgcatgg acctccgcgc gcccggaggc cttacgccgc
      121 cggcccgtcc ttcttcttgc cgccgatctt gccctcggtg ccggcgcgca ggcgggcgat
      181 gttctccttg tggcgccaga acagcaccgc cgtcatcacc gcgagcaggc cgaccgccgg
      241 cttggcgccg aggaagagca ggcccgcgag gggcgcggcg agcgccgccg acagcgccgc
      301 ggccgaggag tagcgcgtgg tgtaggccac catcagccag atcaccgcgc agaagatcgc
      361 gatgcgtgcg tcgaccccgg cgagtacgcc gagcgcggtg gccacgccct tgccgccgtt
      421 gaagcgcagg aagagcgaga acacgtggcc gaggaaggcg gccagcccgg cgagcgcggc
      481 gatgtccggc tcgaagccga ggcgcagcgc cgcccacacc gccagccagc ccttgagcgc
      541 gtcgccgagc agggtgagca cggccgcggc cttgttgccg ctgcgcagca cgttggtcgc
      601 gccggggttg cccgagccgt acttgcgcgg atcttccagg ccgaacaggc ggctggtgac
      661 gatcgcgaag ggaatggagc cgagcaggta ggcggcgacg atcagaagca gggtggacat
      721 cttggctcca ctgcccgccg gcgggcagct cgaaagggac ggaagggggg agggggcatc
      781 cggcacgcct cgcctgtcgc aagccggcac cgatggtgtg cgacagcttt tccgggcgcg
      841 cgcgcggggg cgcgttagaa tccggacgat tctaaacggg aagcagcatg gatttcatct
      901 tcatcgagga actgcgggtc aaggcctggg tcggcatcta cgcgcgcgag aagaccggcc
      961 cgcagacggt cgagctgaac ctcaccttcg gcgttcccga cgccgctgca gagcacgacg
     1021 acatcgccga caccatcgac tatgcggtgg tgaccgagcg cattcgccgc gagctcggcg
     1081 agcgccactt caacctcatc gagagtctgg gcgagttcgt cgtcaagctg ctgttcgagg
     1141 agttcggcgc gccctgggtc aaactcaagg tgggcaagat cggggtgatg aaggatgtgc
     1201 gccgcgtcgg cgtctatatc cagcgcagca aggcgggtgt ggtcgtaccg ccggccgcct
     1261 gagcgcccgc gggcgctcgc ttcagttcgc cacctgcacc agcctgggct ggagcacgcg
     1321 cagcaggtcg tgcgggtgga tcccgaccag gaagccgcgc cgcccgccgt tgatgtagac
     1381 cagcggcagg tcgagcacgg tgcgctccat gtacaccggc atcttcttgc gcgtgccgaa
     1441 gggcgaggtg ccgcccacca gatagccgct gtggcggttc gcggtctccg gcttgcaggt
     1501 ctcgacgtgc ttgcacggga tctggcgcgc gagctccttg gtggacacct tgtggtcgcc
     1561 atgcatcagc accaccatcg gctgcgcgtt ctcgtcctcc atgatcaggg tcttgaccac
     1621 cgcgtgctcg ggcacgttga gttcgcgcga ggacaccgtg gtgccgccgt gctcctcgta
     1681 ttcgtagagg tggctggaga aggcgacgcc gtgctggcgc aggaagcggg tggcgggggt
     1741 ctcgggggcg tgggcttcgt ttctgctcat gatcggacgg cctgtcgggc gcgccgccgc
     1801 gagcgggcgg cgcggtctcc acattgtagt gccatccgcc atgccggggc gtatggacgc
     1861 gtcgccccgg ccgcggccgg cgcgctcatg cgcgcggatg gtggcgcgcg tgcagctgct
     1921 tgaggcgctc gcgcgcgacg tgggtgtaga tctgcgtggt cgagatgtcg gcgtggccga
     1981 gcagcagctg caccacgcgc aggtcggcgc cgtggttgag caggtgggtg gcgaaggcgt
     2041 ggcgcagggt gtggggcgag atgcgctcgg gcgcgatgcc ggcggcgagg gcgtagtgct
     2101 tgatcaggcg ccagaacatc tggcgcgaca tcgccgcccc caggcgggtc acgaagatct
     2161 cgtcgcagct gcggccggcg agcaggaccg ggcgtgcctc ggccaggtag cgctgcagcc
     2221 agtcggcggc gatctcgccg agcggcacca ggcgctcctt gctgcccttg cccatgaccc
     2281 gcaccacgcc ctcattcacc cccaccgcaa agatcttcag gccgaccagc tcggagaccc
     2341 gcaggccggc ggcgtagagc acctccagca tgcagcggtc gcgcaggcct tgcggggtgt
     2401 cgagcgcggg agcggcgagc agggcctcga cctggggctc gctcagggtc ttgggaaagc
     2461 gttcggccgc gcgcggggtg tcgatcgcca gcgtggggtc ctcggcgatc tcgcggttgg
     2521 cgaggagcag gcggtaatag cgccgccacg cggcgatcag ccggcgttgg ctcgccggct
     2581 tcgcggcgcg gctgaattcg gcgaggtagg cggcgaggtc cgcgccggcg gcggcgggca
     2641 ggcgttgcgc acgcgtctcc agccaggcgg cgaagcgggc gaggtcgctg cggtagccgg
     2701 cgagggtgtt cctggccagc ccgtgctcga gccagagcgc atcggcgaag cggtcgagct
     2761 cggcgcggtg cgcgggctgc aggccggctt cgcgtgccgg cgcgcgtccg ctcacagcgc
     2821 cccttcaaaa gcgagcagcc agcgcttgac gtcgaggagc cagccttcgc gggcgttggc
     2881 gaagccaccg agcccggcgg ccgaggtcac gcggtggcag ggcacgatca ggggaaaggg
     2941 gttggcgccg caggcctggc cgaccgcgcg cggcgcgctg tccaggctgg cggcgagctc
     3001 gccgtaggtt gcggtgcgtc cgcgcgggat tgccgcgatg gccgtccata cgcgacgacg
     3061 gaagggcgtg ccgcagggtg cgagcggcac cgggaagggc tggtcggggt cgtcgatcca
     3121 ggcgcgcagc gcgtgcgccg cggcgccggc gctggcgttg cgcggttcgt gcaagggcgt
     3181 gcccggaggc aggaagacga gctcctgcac ctgcccgtcg tgcgcgcgga tgccgaaggg
     3241 gccgaagggc agcgcgatga tcgcgtcgaa ggccgacgtg tccgcgccgc ccgtcgtcgt
     3301 gacgggcgcc cggctcactg cggtgtcttg agcagcgcct cgcgcagctc gctgtcggcc
     3361 gggcgctcgc ccagccagat gcgcagcagc gcgggataga aggcggcgtc ggcgatgtcc
     3421 tcgccctgct gcttcgtatc gaccagcagc cgggtggcgc cgttgccgag gcgatcgagc
     3481 acgatctcgg tgccggcctt gacctcgccg accgcggcga gcgcgcccag cagcgcgtcg
     3541 gtggccggct tgagcgcggc cagcgcggcg gcgtcgtggt tcttctcgag cccggtctgc
     3601 agggcatcgc cgaactgctt cgcctcgagg tcgcggatcg gcacgatgcg gatgcgcttc
     3661 tcgcctttgt cggccaggat cgcgccggcc tcggcgagcg gggcgcgcag gtacaggccc
     3721 atcgcataga ccttgaagcc gagccgggtg cgcaggccgg cgccgttgag ctcgagcgtg
     3781 cgctgctgca cggtctgctg cggggcgaac tcgacgttgc cgaccttgac ggcggcctgg
     3841 gcgaggctgg ccgagagcag ggcggcgacg atcaggctgc ggaggatgcg gatgctcatg
     3901 gggttccttg tcggggtggt tccatttggc agtgtcagcg gcggacttcg ccgttgccga
     3961 acacgatcca tttctggctg gtaaggccct ccaggccgac cgggccgcgg gcgtggatct
     4021 tgtcggtgga gatgccgatc tccgcgccga ggccgtactc gaagccgtcg gcgaagcggg
     4081 tggaggcatt caccatcacc gaggatgaat ctacctcgcg caggaagcgc atggcgtggg
     4141 aatagttctc ggtgacgatc gcttcggtgt ggcccgaact gcaggtgttg atgtg
//
LOCUS       DAOQNN010000284         4193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13487_length_4193_cov_10.395602, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000284 DAOQNN010000000
VERSION     DAOQNN010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13487_length_4193_cov_10.395602"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1087)
                     /locus_tag="PKV97_13020"
     CDS             complement(<1..>1087)
                     /locus_tag="PKV97_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB06847.1"
                     /translation="FGDVEDYAGRVMLLIDGYAVQDNLFDQAYIDESGLIDLELVDRV
                     EYVPGTGSVTYGNNALLGILNVVTRRGRDFDGARVSAEISSRGASRQRATWGKHFNNG
                     AEVLLSASTLDVDGRNLYFPAYDTPATNFGVAEGLDGERNQRVFGKLSWSGWTVQAAW
                     VEREKSVPTNPSAYTAFNTPFPTRDESAFLGVRHETDLGLQLYSSSSLMLGRYAYWNQ
                     REYALDEDGEYDDGEKYGVRDYHGAWWRFDQKFVGRWFTDHTLVFGAELRDDHRQSFH
                     RRFLSPAGEVTDRDDGELSRRTFSLYVADDYRLNQQWTLNLGVRHDDADDLDGNLSPR
                     AALIWQQDPATTWKASYSEAFKMPNAN"
     assembly_gap    1088..1187
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1188..2897
                     /locus_tag="PKV97_13025"
     CDS             <1188..2897
                     /locus_tag="PKV97_13025"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB06848.1"
                     /translation="VASVTEAIMRLGMGTVRNLALGFSLVDQYQNGPCGQFDYQSFWS
                     HSLLMGLAMQRFSNMTGVGSSEELFACGLLAQVGRLSLATVYPVEYGRLLDGQDGAAR
                     SLVALEQEHLQTDHNELTAALLGEWGIPGALIEPIYHHEDPESSGFSRGSRPYQLVHL
                     FYLARRIADLGLAGESERNACTAELLLLGGRIGLDAEQLGATVDDLVQQWRTWGELLR
                     VPASALPSFAHMSAAHAMRDEKSDGAAALRVLLVDDDPTTRCMMEGVLSGALGHVMVC
                     ANNGREALALAVETQPQIVVTDWMMPGMDGLDLARALRATEWGQTMYLIMLTGHENED
                     EVSEAFEAGVDDFLVKPVNVRTLRARLRAARHYVQLLEAWERDRAQLKQFAAELAISN
                     RRLAHAAMTDLLTNLPNRRSGMNALAKAWAASSRFEQPLSVMMLDIDHFKRINDSHGH
                     AVGDTVLRDVAQAIQQSARKDDSMCRMGGEEFLVICPNTDLKAAVQAAERLRRMVERL
                     RIQAGETEIQTSVSIGVATREVGMTETDALVNAADRALYRAKQAGRNRSCVVVQGQMR
                     CFP"
     gene            complement(3106..>4193)
                     /locus_tag="PKV97_13030"
     CDS             complement(3106..>4193)
                     /locus_tag="PKV97_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNB06849.1"
                     /translation="ADDNGMLLLDLKDLRAMVQYVGENAKSFTTEYGNISSASIGAIQ
                     RNLLALEEQGGDVFFGEPMLDIADLMQTDADGRGVINVLAADKLYHSPKLYSTFLLWM
                     LSELFEQLPEVGDPDKPKMVFFFDEAHLLFNDAPKALIEKIEQVVRLIRSKGVGVYFV
                     TQNPLDVPDTVLGQLGNRVQHALRAFTPRDQKAVKTAADTMRPNPAFDAVAAITELGV
                     GEALISFLDEKGRPQITERCNVIAPDSRLGPLEPGERRAAIAGSVIYGHYEKAVDRES
                     AYELLKASAATKAAVEAGRAAAKEVGGEDTGSVVSDILFGATGPRGGKRDGLVQIAAK
                     TVTRTIGSSLGRQIVRGILGSLLGGKR"
BASE COUNT          624 a         1381 c         1435 g          653 t
ORIGIN      
        1 tcgttggcgt tgggcatctt gaaggcctcg ctgtacgaag ccttccaggt cgtcgccgga
       61 tcctgctgcc agatcaacgc ggcacgcggg ctgaggttgc cgtcgagatc gtcggcgtcg
      121 tcgtgacgca cgcccaggtt gagcgtccac tgctggttca gccggtagtc gtcggcgacg
      181 tagagactga aggtgcggcg cgagagctcg ccgtcgtcac gatccgtgac ttcgccggcg
      241 ggcgagagga agcggcggtg gaaggactgg cggtggtcgt cgcgcaactc cgccccgaat
      301 accagcgtgt ggtcggtgaa ccagcgcccg acgaacttct ggtcgaagcg ccaccacgcg
      361 ccgtggtagt cgcgcacgcc gtacttctcg ccgtcgtcgt actcgccgtc ctcgtcgagg
      421 gcgtattcgc gctggttcca gtaggcgtag cgtcctaaca tcagactgga cgaggaatac
      481 agctgcagac cgaggtcggt ctcgtggcgc accccgagga aggcgctctc gtcgcgcgtc
      541 gggaacggcg tgttgaaggc ggtgtatgcg gaggggttcg tgggtacgct cttctcgcgc
      601 tccacccacg ccgcctgaac ggtccagccc gaccacgaca gctttccgaa cacccgctgg
      661 ttacgctcgc cgtcgagccc ctcggccacg ccgaagttgg tcgcgggcgt gtcgtaggcg
      721 gggaagtaca ggttgcggcc atcgacgtcg agcgtcgagg ccgagagcag gacctcggcg
      781 ccgttgttga aatgcttgcc ccaggtggcg cgctggcggc tggcgccgcg gctggagatc
      841 tccgcggaca cgcgcgcacc gtcgaagtcg cgcccgcgcc gggtgacgac attgaggatg
      901 ccgagcagcg cgttgttgcc gtaggtcacc gagccggttc ccggcacgta ctcgacgcga
      961 tcgaccagtt ccaggtcgat caggccggat tcgtcgatgt aggcctggtc gaagaggttg
     1021 tcctgcaccg cgtagccgtc gatcagcagc atcacgcggc cggcgtagtc ctcgacgtcg
     1081 ccgaagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtg gcctcggtca
     1201 ccgaggcgat catgcgcctg ggcatgggca cggtgcgcaa cctcgcgctc ggcttctcgc
     1261 tggtggacca gtaccagaac gggccctgcg gacagttcga ctaccaaagc ttctggtcgc
     1321 attcgctgct gatggggctg gcgatgcaga ggttcagcaa catgacgggg gtcggctcga
     1381 gcgaggagct cttcgcctgc ggcctgctcg cccaggtggg ccggctctcg ctcgccaccg
     1441 tctatccggt cgagtacggc cgcctgctcg acggccagga cggcgccgcg cgcagcctgg
     1501 tggcgctcga gcaggagcac ctgcagaccg atcacaacga gctcaccgcc gcgctgctcg
     1561 gcgagtgggg catccccggc gcgctgatcg agccgatcta ccaccacgag gacccggaaa
     1621 gctccggctt ctcgcgcggc tcacggccct accagctggt acatctcttc taccttgcgc
     1681 gccgcatcgc cgacctcggt ctcgccggcg agagcgaacg caacgcctgc accgccgagt
     1741 tgctgctgct cgggggcagg atcggcctcg acgccgaaca gctcggcgcc acggtggacg
     1801 acctggtgca gcagtggcgc acttggggcg agctgctgcg cgtgccggcc tcggcgctgc
     1861 cctcgttcgc gcacatgtcg gcggcgcacg cgatgcgcga cgagaagagc gatggcgcgg
     1921 cggcgctgcg cgtgctgctg gtcgacgacg accccaccac gcgctgcatg atggagggcg
     1981 tgctgtccgg ggcgctgggc cacgtgatgg tgtgcgccaa caacggtcgc gaggcgcttg
     2041 cgctggcggt cgaaacccag ccgcagatcg tcgtcaccga ctggatgatg ccgggcatgg
     2101 acgggctgga cctcgcgcgt gctctgcgtg cgaccgagtg ggggcagacc atgtacctga
     2161 tcatgctcac cgggcatgaa aacgaggacg aggtgagcga ggccttcgag gccggggtgg
     2221 acgatttcct ggtgaagccg gtcaacgtgc gcaccctgcg cgcgcgtctg cgcgccgccc
     2281 ggcactacgt gcagctgctc gaggcgtggg agcgcgaccg tgcccagctc aagcagttcg
     2341 cggcggagct ggcgatcagc aaccgccgcc tcgcccacgc ggcgatgacc gacctgctca
     2401 ccaacctgcc caaccgccgc tcgggcatga acgcgctcgc caaggcctgg gccgcctcca
     2461 gccgcttcga acagccgctg tcggtgatga tgctcgacat cgaccacttc aagcgcatca
     2521 acgacagcca cggccacgcg gtcggcgaca ccgtgctgcg cgacgtcgcc caggccatcc
     2581 agcagtcggc gcgcaaggac gacagcatgt gccgcatggg tggcgaggaa ttcctcgtga
     2641 tctgcccgaa caccgacctc aaggcggcgg tccaggccgc cgagcgcctg cgccgcatgg
     2701 tcgagcgcct gcgcatccag gccggcgaaa cggaaataca gacctcggtg agcatcggcg
     2761 tggccacccg cgaggtcggc atgaccgaga ccgacgcgct cgtcaatgcc gccgatcgcg
     2821 ccctctaccg cgccaagcaa gccggccgca accgcagctg cgtggtggtg caggggcaga
     2881 tgcggtgttt tccgtgaggc gtcgggaacc ggcctgcgcg cggcgcgaat cggccgcggc
     2941 agcgctgaag cgcgggatcg cgacttgcgt cgctcctacg agaccgccgc gctcccgaag
     3001 gcgcctgtag gagcgacgca agtcgcgatc cggcaatgga caccgcggcg cgcggcgctt
     3061 gcccgcgcgg ccgccggaag ggatgcaatc cccctcccgt ccccttcagc gcttgccgcc
     3121 cagcagcgag cccaatatcc cgcgcacgat ctgccgcccc aggctgctgc cgatggtgcg
     3181 cgtgaccgtc ttggcggcga tctgtaccag tccgtcgcgc ttgccgccac gcgggccggt
     3241 ggcaccgaag aggatgtcgc tgaccacact gccggtatcc tcgccgccga cctccttcgc
     3301 cgccgcgcgg ccggcctcga ccgcggcctt ggtcgcggcg ctggccttga gcagctcgta
     3361 ggcggattcg cggtcgaccg ccttctcgta gtggccgtag atcaccgagc cggcgatcgc
     3421 cgcccggcgc tcgccgggct cgagcgggcc gaggcgcgaa tcgggcgcga tgacgttgca
     3481 gcgctcggtg atctgcggcc ggcctttttc gtcgaggaag gagatcagcg cctcgcccac
     3541 gccgagctcg gtgatcgccg caacggcgtc gaaggccggg ttggggcgca tggtgtcggc
     3601 ggcggtcttc accgccttct ggtcgcgcgg ggtgaaggcg cgcagcgcgt gctgcacgcg
     3661 gttgccgagt tggccgagca cggtgtcggg cacgtcgagc gggttctggg tgacgaaata
     3721 cacgcccacg cccttggagc ggatcaggcg caccacctgc tcgatcttct cgatcagcgc
     3781 cttgggcgcg tcgttgaaca gcaggtgggc ctcgtcgaag aagaacacca tcttcggctt
     3841 gtcggggtcg ccgacctcgg gcagctgctc gaagagctcg gacagcatcc acagcaggaa
     3901 ggtggagtag agcttgggcg agtggtagag cttgtccgcg gcgagcacgt tgatcacgcc
     3961 gcggccatcg gcgtcggtct gcatcaggtc ggcgatgtcg agcatcggct cgccgaagaa
     4021 cacgtcgccg ccctgctctt ccagcgccag caggttgcgc tggatggcac cgatcgaggc
     4081 gctggagatg ttgccgtact cggtggtgaa gctcttcgcg ttctcgccca cgtactggac
     4141 catcgcgcgc aggtccttga ggtcgagcag cagcatgccg ttgtcgtcgg cga
//
LOCUS       DAOQNN010000285         4178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13556_length_4178_cov_7.468106, whole genome shotgun sequence.
ACCESSION   DAOQNN010000285 DAOQNN010000000
VERSION     DAOQNN010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13556_length_4178_cov_7.468106"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1288
                     /locus_tag="PKV97_13035"
     CDS             143..1288
                     /locus_tag="PKV97_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNB06850.1"
                     /translation="MSADEGRAALVLTGGGARAAYQVGVLAAVRELRGKRPGNPFPIL
                     CGTSAGAINAVALAVHAGDFGRAVRHIQRVWRSLHVDQVYRVDPLALFGSGLRWGAAL
                     FGGWLVRQTPRSLLDNTPLRGLLERVLDFPAIEAAIGRGDLRALSVTASGYSSGESLA
                     FFQGAPELQPWRRAQRLGVRAELDVCHVLASAALPFVFPAVKIHREYFGDGSMRQLAP
                     VSPAIHLGADRILVIGAGRTAEEGRVRAAGGYPSPAQIAGHALSSIFLDTLEVDLERL
                     ARINDTVGRLDEARRAEAGIALRPLECLVIQPSQRLDIIAARHAHSLPAQLRIVLRGL
                     GTFRREGSTLLSYLLFEPSFTRALIELGYHDAMTRAGELRRFLRIDS"
     gene            1449..2615
                     /gene="pbpG"
                     /locus_tag="PKV97_13040"
     CDS             1449..2615
                     /gene="pbpG"
                     /locus_tag="PKV97_13040"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HNB06851.1"
                     /translation="MKVRPLIAAIAAMFALQSGMAIAAPKASGKAATSKVSKPAAKKA
                     AATAPVRKAAVKPAPKKGVVAKAAASDTKAARSAKAATRASANARGKAPTMRVSLRKP
                     AVAAAALAAPAAVLADEPDTPVTLDGSGMPVLASNAFYVINQRTGEVLLERNATEVLP
                     IASITKLMTAMVVLDAGLSLSEPIEINDADIDQLKGTGSRLALGTTLSREEMLQLALM
                     SSENRAASSLARHYPGGEAAFVDAMNVKARLLGLWNTRFHDSTGLNPANVSSPRDLAK
                     MVAASASYPLIREFSTAHERYVPVHGRMLRFGNTNSLVRSPEWTISVSKTGYISEAGR
                     CLVMQTWLHQQPVVMVLMDSSGRYTRTADAKRVRKWLEQTAPQRLAAAPQGAGG"
     gene            2846..>4178
                     /locus_tag="PKV97_13045"
     CDS             2846..>4178
                     /locus_tag="PKV97_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNB06852.1"
                     /translation="MGAALPETAPSRLFLSGNEAVALAVRDAGCRVAAAYPGTPATEI
                     LEELSRFPDLYTEWSVNEKVSLEVALGASMVGARAFCAMKHVGLNVAADALMTMTVTG
                     TEGGLVIAVADDVGMSSSQNEQDSRFWARFAHLPLFEPADAQESYDMAREAFAVSERF
                     RCPAIVRLTTRICHVKGRVVAGEREAHEPAGFVKDPQRWVMVPGHAKPRVALMYEREE
                     ALRAASAQSPFNLLVEGSDRRIGFVTSGPAYMHVRETFPDAPVFKLGQSYPLPLERLR
                     EFAAGVDQLLVVEETEPLVESELRAGGIACTGKDVLPRVGELSPDRLRPAVARLRGEE
                     IPVTAQPRLVPQQVFPRPPTMCVACPHLGIYYTLAQLRNLTISGDIGCYTLGAGHPWN
                     ALDTCISMGASMGVALGMDKGRGQADAKKAVIAVIGDSTFMHMGMQGLLDIT"
BASE COUNT          577 a         1584 c         1403 g          614 t
ORIGIN      
        1 gtaggagcga cgccagtcgc gatcgcggcg cttccgcacc gaccacgccc ctcgctaccg
       61 caccgcgccc gcgcattcct tccttgtccg gtcggcctgc gctgcgcgaa gctgacgcca
      121 tcccatggcc ggaggcgggc ggatgagcgc ggacgaaggg cgggcggcgt tggtgctgac
      181 cgggggtggc gcgcgcgccg cctaccaggt cggcgtgctg gctgcggtcc gcgaactgcg
      241 cggcaagcgc cccggcaacc ccttcccgat cctgtgcggc acctcggcgg gggcgatcaa
      301 cgcggtcgcg ctcgccgtgc acgcgggcga cttcggccgc gcggtgcggc acatccagcg
      361 cgtctggcgc agcctgcacg tggatcaggt gtaccgcgtc gatccgctcg cgctcttcgg
      421 cagcggcctg cgctggggcg cggcgctgtt cggcggctgg ctggtccggc agacgccacg
      481 ctcgctgctc gacaacacgc ccttgcgcgg cctgctcgag cgcgtgctcg acttccccgc
      541 gatcgaggcc gcgatcggcc gcggcgacct gcgcgcgctc agcgtcaccg cctcgggcta
      601 ttcctccggc gagagcctcg ccttcttcca gggggcgccc gagctgcagc cctggcggcg
      661 cgcgcagcgc ctgggcgtgc gcgccgagct cgacgtctgc cacgtgctgg cgagcgcggc
      721 gttgcccttc gtcttccccg cggtgaagat ccatcgcgag tatttcggcg acggctcgat
      781 gcgccagctc gcgccggtga gcccggcgat ccacctcggc gccgaccgca tcctggtgat
      841 cggcgccgga cgcaccgccg aggaggggag agtgcgtgca gcgggcggct acccgtcgcc
      901 ggcgcagatc gccggccacg ccttgtcgag catcttcctc gacaccctcg aggtcgatct
      961 cgagcgcctc gcgcgcatca acgacacggt gggccggctc gacgaggccc gccgcgccga
     1021 ggcggggatc gcgctgcgtc cgctcgagtg cctggtgatc cagccctcgc agcgcctcga
     1081 catcatcgcc gcgcgccatg cgcacagcct gccggcacag ctgcgcatcg tgctgcgcgg
     1141 gctgggcacc tttcgccgcg agggctcgac cctgctctcc tacctgcttt tcgaaccgag
     1201 cttcacgcgc gcgctgatcg agctcggcta tcatgacgcg atgacgcgcg ccggcgaact
     1261 gcgccgcttc ctgcgcatcg actcctgacc cggggcgggg ggtggcgagc ggactcgccg
     1321 gcgacgccgc tgcgggagta gaattccgcc agagttggaa acaacttgaa atccatttcg
     1381 tatctgccta ttttttaaag cagaacgcct tgaatccgcc ttccaccgct ctccgtcccg
     1441 tcgccatcat gaaagttcgc cccctgatcg ccgcgattgc cgcgatgttc gcgctgcagt
     1501 ccggcatggc catcgcagcc ccgaaggcat ccggcaaggc tgcgacgagc aaggtttcca
     1561 agccggccgc gaagaaggcc gcggccaccg ccccggtgcg caaggccgcc gtcaagcccg
     1621 cgccgaagaa gggcgtcgtg gccaaggccg cggcgtccga caccaaggcg gcccgttccg
     1681 ccaaggctgc gacccgtgcg tccgccaacg cccgcggcaa ggcgccgacg atgcgggtga
     1741 gcctgcgcaa gccggcggtc gccgccgccg cgctcgccgc ccccgccgcg gtgctcgccg
     1801 acgaaccgga taccccggtc acgcttgacg gcagcggcat gcccgtgctc gcttcgaacg
     1861 ccttctacgt gatcaaccag cgcaccggcg aggtgctgct cgagcgcaac gccaccgaag
     1921 tgttgccgat cgcctcgatc accaagctga tgaccgcgat ggtggtgctc gatgcggggc
     1981 tgagcctgtc cgagccgatc gagatcaacg acgccgacat cgaccagctc aagggcaccg
     2041 gctcgcggct cgcgctcggc accacgctgt cgcgcgaaga gatgctgcag ctcgcgctga
     2101 tgtcgtccga aaaccgcgcg gcctcgtcgc tggcccgcca ctatccgggc ggcgaggccg
     2161 ccttcgtcga cgcgatgaac gtcaaggcgc gcctgctcgg gctgtggaac acccgcttcc
     2221 atgacagcac cgggctcaac ccggccaacg tctccagccc gcgcgacctc gccaagatgg
     2281 tcgcggcctc ggcgtcctac ccgctgatcc gcgagttctc caccgcgcac gagcgctacg
     2341 tgccggtgca cggccgcatg ctgcgcttcg gcaacaccaa ctcgctggtg cgcagcccgg
     2401 aatggacgat cagcgtgtcc aagaccggct acatctccga ggcggggcgc tgcctggtca
     2461 tgcagacctg gctgcaccag cagccggtgg tcatggtgct gatggactcc tccggccgct
     2521 acacccgcac cgccgacgcc aagcgcgtgc gcaagtggct cgaacagacc gctccccagc
     2581 gcctggcggc cgcaccgcag ggcgcgggcg gctgagcttc ccgattacgc aggtcgaacg
     2641 acgcggggcc gcccggtggg gcaggccccg cgtcgcgttt ggtctgatcg agcgcactgc
     2701 cgctgcgggc gcggcatcgg cgcccgtcgc gggtgttccg tggcgaccac gactgcaggg
     2761 cggttgatct tcgtcaatgg agccgggcag ggcgctcact aagctcgcgg gttccgaccc
     2821 cgaacccacc agagaggatc tccccatggg tgctgccctg cccgaaaccg ccccgagccg
     2881 cctgttcctg tccggcaacg aggccgtggc gctcgccgtg cgcgacgccg gctgccgcgt
     2941 cgccgccgcc tatccgggca cgccggcgac cgagatcctc gaggagctgt cgcgctttcc
     3001 cgacctgtac accgagtggt cggtgaacga gaaggtctcg ctcgaggtcg cgctcggcgc
     3061 ctcgatggtg ggcgcgcgcg ccttctgcgc gatgaagcac gtcggcctca acgtcgccgc
     3121 cgacgcgctg atgaccatga ccgtcaccgg caccgaaggc ggcctcgtga tcgcggtcgc
     3181 cgacgacgtc ggcatgtcct cgtcgcagaa cgagcaggac tcgcgcttct gggcccgctt
     3241 tgcccacctg ccgctgttcg agcccgccga cgcgcaggag tcctacgaca tggcgcgcga
     3301 ggccttcgcg gtgtccgagc gcttccgctg cccggcgatc gtgcgcctga ccacccgcat
     3361 ctgccacgtc aagggccgcg tcgtcgcggg cgagcgcgag gcccacgagc ccgccggctt
     3421 cgtcaaggat ccgcagcgct gggtcatggt gcccggccac gccaagccgc gcgtggcgct
     3481 gatgtacgag cgcgaggagg ccttgcgcgc cgcctccgcg cagagcccgt tcaacctgct
     3541 cgtcgagggc agcgaccgcc gcatcggctt cgtgacctcg ggcccggcct acatgcacgt
     3601 gcgcgagacc ttccccgacg cgccggtgtt caagctcggc cagtcctacc cgctgccgct
     3661 cgagcgcctg cgcgagttcg ccgccggcgt cgaccagctc ctggtggtcg aagagaccga
     3721 gcccctggtc gagagcgagc tgcgcgccgg cggcatcgcc tgcacgggca aggacgtgct
     3781 gccgcgcgtc ggcgagctct cgcccgaccg cctgcgcccg gccgtggccc gcctgcgcgg
     3841 cgaggaaatc cccgtcaccg cgcagccccg cctggtgccg cagcaggtct tcccgcgccc
     3901 gcccaccatg tgcgtcgcct gtccgcacct gggcatctat tacaccctcg cgcagctgcg
     3961 caacctcacc atctcgggcg acatcggctg ctacaccctg ggcgccggcc acccctggaa
     4021 cgcgctcgac acctgcatct ccatgggcgc ctcgatgggc gtcgcgctcg gcatggacaa
     4081 gggccgcggc caggccgacg ccaagaaggc ggtgatcgcc gtgatcggcg actcgacctt
     4141 catgcacatg ggcatgcagg gcctgctcga catcacct
//
LOCUS       DAOQNN010000286         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13595_length_4170_cov_10.982017, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000286 DAOQNN010000000
VERSION     DAOQNN010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13595_length_4170_cov_10.982017"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..876)
                     /locus_tag="PKV97_13050"
     CDS             complement(262..876)
                     /locus_tag="PKV97_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06853.1"
                     /translation="MSSERFPAFFDQVPRLVVRDPLAEFLGAAEGGVLEYGYADAVRL
                     AGHSCPTVASAYALAHHAMTRLFPGALPVRGLVQVGFQHPLDEGVTGVVAAVVGLLTG
                     SAQDGGFKGIAGRFVRRNLQRFGEPQPLALRFSRSDTGASVDAAAELAKVQPHPEMAE
                     RMGRCMRGDADAGELQRFGQLWQDRVRRVLVEHWDDPEVFHFSG"
     gene            complement(944..1771)
                     /locus_tag="PKV97_13055"
     CDS             complement(944..1771)
                     /locus_tag="PKV97_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNB06854.1"
                     /translation="MQDIPNPSPSTARDAAESAAERRGIQSIEVGGSLLSALVREGKP
                     MMLKDLAREAGMPAAKAHPYLVSFGKLGLVEQDALTGRYGLGPFALQMGLSALHGLDP
                     IKAALPEAARLADDIELNVAIAVWGNHGPTVVHIEECSRQIHINMRPGTVMTPLLLSA
                     TGRVFAAFLPQRIVAPVLAAEAAAVGAPPLEMSAADVRAVLDEIRTQRLARALGNPIP
                     GIHAFSAPVFDGGGQLALAITAMGPVGSVDPDWEGLTACRVRAAAKAIERRLGKQAA"
     gene            1918..2883
                     /locus_tag="PKV97_13060"
     CDS             1918..2883
                     /locus_tag="PKV97_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB06855.1"
                     /translation="MSTKKFASQADLEEKKISWEKLSDNAYAYTAEGDPNTGVIIGDD
                     GVMIIDATATPVAAQGVIAKVREITDLPIKYVVLTHYHAVRVLGASGYKAEGLEQIIA
                     SKDTWDLIVERGQQDMDSEIGRFPRLFQAVESVPGLTWPTMTFSGEMTVWMGKLEVRI
                     KQLGRGHTKGDTIVYLPAQDICFSGDLVEADAACYTGDAYLADWPQTLDNLAAMNFAK
                     LVPGRGPALMTPEKVQAGLAYTRDFVSTLYRSAQEAVAKGMDLKATMAHTRANMDPKF
                     GQVFIYEHCLPFDVTRAHDEASGIRDPRIWTAERDQQMWHALQQG"
     gene            3039..>4170
                     /locus_tag="PKV97_13065"
     CDS             3039..>4170
                     /locus_tag="PKV97_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB06856.1"
                     /translation="MLSTYQYPKFEYVRPPEIAEGRDGHYPVVIVGAGPVGLCTAIDL
                     ARQGRQVLLLDNDDTVSIGSRGVCYAKRTLEVLDRLGCGEEMVQKGVSWNVGRTFFRE
                     DEVFNFNLCPEPDHHRPGMINLQQYYLEDYLVQRARKLPNLEMRFRNNVIAVTPGEDK
                     ASLRVETPDGAYTLTTDWLVVADGARSPIRTMLGLDIEGKVFMDRFLIADVVMKADFP
                     TERWFWFDPPFHPNQSVLLHRQADNVFRIDFQLGWQADPEQEKKPENVIPRIQAMIGD
                     DREFELEWVSVYTFQCRRMNRFNHGRVLFVGDSAHQVSPFGARGANSGIQDADNLVWK
                     LALVMDGKAPATLLDTYGDERGFAADENIMNSTRSTDFITPKS"
BASE COUNT          680 a         1476 c         1422 g          592 t
ORIGIN      
        1 aggccgaccg gggcgatctg cgccacctga tcccggccgc gctgctcggc atcggcgcgg
       61 ggacgctgct ctgcggcgtg ctctccgacc gcatggtcaa gctcgtgctc gggctgatcg
      121 tgctcgcctt cgttgccgac cgcctgctgc gccggcgcac cgcggtcgcc gcgaccggcg
      181 aaggaccgcg cgtgctgccc ttcgcccggt cgtgcggcgc caagctgcta tgggacgggc
      241 tgggcggcgg aagccggccg atcagccgga aaagtggaac acctcggggt cgtcccagtg
      301 ctcgacgagc acgcgccgga cgcggtcctg ccacagctgt ccgaagcgct gcagttcgcc
      361 ggcgtcggcg tcgccgcgca tgcagcggcc catgcgctcg gccatctcgg ggtggggctg
      421 cacctttgcc agttcggcgg cagcgtccac ggaggcgccg gtgtcgctgc ggctgaagcg
      481 cagcgcgagc ggctgcggtt cgccgaagcg ctgcaggttg cggcgcacga agcgcccggc
      541 gatgcccttg aagccgccgt cctgggcgct gccggtgagg agcccgacca ccgcggcgac
      601 gacgccggtc acgccctcgt cgagaggatg ctggaagccc acctgcacca agccgcgcac
      661 gggcaatgcg ccggggaaga gccgcgtcat cgcgtgatgg gcgagggcgt aggccgaggc
      721 caccgtcggg caggaatggc cggccaggcg caccgcgtcg gcgtagccgt actcgagcac
      781 gcccccctcg gcggcgccga ggaactcggc gagcggatcg cgcaccacga ggcgtggcac
      841 ctggtcgaag aaagcgggaa aacgttcgct ggacatgctc gctccggggc gttggggagc
      901 gccgattctc cgtccgcgcc ggtgtcggcc gccttgacgt ggctcaggcc gcctgcttgc
      961 cgaggcggcg ctcgatcgcc ttcgctgccg cgcgcacccg gcatgcggtg aggccttccc
     1021 agtccggatc gacgctgccg accggtccca tcgcggtgat cgcgagcgcg agctggccgc
     1081 cgccgtcgaa gaccggcgcg gaaaacgcat ggatgcccgg gatcggattg ccgagtgcgc
     1141 gcgcgaggcg ctgcgtgcgg atctcgtcga gcaccgcccg gacgtcggcg gcggacatct
     1201 ccagcggtgg ggcgcccacc gcagccgctt cggcggcgag cacgggggcg acgatgcgct
     1261 gcggcaggaa ggccgcgaac acgcgcccgg tggccgacag cagcaagggc gtcatgaccg
     1321 tgccgggccg catgttgatg tggatctggc ggctgcactc ctcgatgtgc acgacggtgg
     1381 ggccgtggtt gccccacacc gcgatcgcca cgttgagctc gatgtcgtcg gcgagccggg
     1441 cggcttcggg cagggccgcc ttgatcggat cgaggccgtg cagcgcgctc aggcccatct
     1501 gcagcgcgaa cgggccgagc ccgtagcggc cggtgagcgc gtcctgctcc accaggccga
     1561 gcttgccgaa gctcaccagg tagggatgcg ccttggccgc cggcatgccg gcctcgcgtg
     1621 cgaggtcctt cagcatcatc ggcttgcctt cgcgcaccag ggcggacagc aggctgccgc
     1681 cgacctcgat ggactggatg ccgcggcgct cggcggcgga ctcggccgca tcgcgtgcag
     1741 tcgaggggga gggattaggg atgtcctgca tgctgctctc cggattcgcg gcgcatctta
     1801 acagtccacc ggactggccg cgcatccgga agatttgcct ataacaaacg tattgactat
     1861 taacgaactc acaactagta tggatccatc gctgcacatc accggagacc tcccctcatg
     1921 agcaccaaga aattcgcctc ccaggccgac ctcgaagaga agaagatcag ctgggagaaa
     1981 ctgtccgaca acgcctatgc ctataccgcc gagggcgacc ccaacaccgg cgtgatcatc
     2041 ggcgacgacg gcgtgatgat catcgacgcc accgccaccc cggtggccgc gcagggcgtg
     2101 atcgccaagg tgcgcgagat caccgacctg ccgatcaagt atgtggtgct cacccactac
     2161 cacgcggtgc gcgtgctcgg cgcctcgggc tacaaggccg agggcctgga gcagatcatc
     2221 gccagcaagg acacctggga cctcatcgtc gagcgcggcc agcaggacat ggactccgag
     2281 atcggccgct tcccgcgcct gttccaggcg gtcgagagcg tgcccgggct gacctggccg
     2341 accatgacct tcagtggcga gatgaccgtg tggatgggca agctcgaggt ccgcatcaag
     2401 cagctcggcc gcggccacac caagggcgac accatcgtct atctgcccgc ccaggacatc
     2461 tgcttctcgg gcgacctggt cgaggccgac gccgcctgct acaccggcga cgcctacctc
     2521 gccgactggc cgcagaccct cgacaacctg gcggcgatga acttcgccaa gctcgtgccc
     2581 ggccgcggcc cggcgctgat gacgccggag aaggtgcagg ccggcctcgc ctacacgcgc
     2641 gacttcgtct ccacgctgta ccgcagcgcg caggaggcgg tcgcgaaggg catggacctg
     2701 aaggcgacga tggcgcacac ccgcgccaac atggacccga agttcgggca ggtcttcatc
     2761 tacgagcact gcctgccctt cgacgtgacc cgcgcccacg acgaggcgag cggcatccgc
     2821 gatccgcgca tctggaccgc cgagcgcgac cagcagatgt ggcacgcgct gcagcagggc
     2881 tgagcctgca gcgggcgggg cgccgtgtgg cgccgcgcct ctcgcacccc tgatttccag
     2941 cagcgtccgg cgccggctgc ggcgccccgc accgcaccgc aggaccgcgc gccaagacgc
     3001 ggccccggcg cacacgaaca aacccggagg agaccgccgt gctgagcacg taccaatacc
     3061 ccaagttcga atacgtccgt ccgcccgaga tcgccgaagg gcgcgacggc cactacccgg
     3121 tggtcatcgt cggcgccggc ccggtgggtc tgtgcaccgc gatcgacctc gcccgccagg
     3181 gccggcaggt gctgctgctc gacaacgacg acacggtgtc gatcggctcg cgcggcgttt
     3241 gctacgccaa gcgcaccctc gaggtgctcg accgcctcgg ctgcggcgag gagatggtgc
     3301 agaagggcgt gtcgtggaac gtcggccgca ccttcttccg cgaggacgag gtattcaact
     3361 tcaacctctg ccccgagccc gaccaccacc gcccgggcat gatcaacctg cagcagtact
     3421 acctcgagga ctacctggtg cagcgcgcgc gcaagctccc gaacctcgag atgcgcttca
     3481 ggaacaacgt gatcgcggtc acccccggcg aggataaggc gagcctgcgc gtggagacgc
     3541 ccgacggcgc ctatacgctc accaccgact ggctggtggt ggccgacggc gcgcgcagcc
     3601 cgatccgcac catgctcggg ctggatatcg agggcaaggt cttcatggac cgcttcctca
     3661 tcgccgacgt ggtcatgaag gcggacttcc ccaccgagcg ctggttctgg ttcgacccgc
     3721 ccttccatcc caaccagtcg gtgctgctgc accgccaggc cgacaacgtc ttccgcatcg
     3781 acttccagct cggctggcag gccgatccgg agcaggagaa gaagcccgag aacgtgatcc
     3841 cgcgcatcca ggcgatgatc ggggacgatc gcgagttcga gctcgagtgg gtcagcgtgt
     3901 ataccttcca gtgccggcgc atgaaccgct tcaatcacgg tcgcgtgctc ttcgtcggcg
     3961 actcggcgca ccaggtctcg cccttcggcg cgcgcggcgc caactccggc atccaggacg
     4021 ccgacaacct ggtgtggaag ctcgcgctgg tgatggacgg caaggcgccc gccacgctgc
     4081 tcgacaccta cggcgacgag cgcggcttcg cggcggacga gaacatcatg aactcgacgc
     4141 gctcgaccga cttcatcacg cccaagagcc
//
LOCUS       DAOQNN010000287         4132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13792_length_4132_cov_8.993623, whole genome shotgun sequence.
ACCESSION   DAOQNN010000287 DAOQNN010000000
VERSION     DAOQNN010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13792_length_4132_cov_8.993623"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..836
                     /locus_tag="PKV97_13070"
     CDS             30..836
                     /locus_tag="PKV97_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNB06857.1"
                     /translation="MTVTLQRLVESAAFQRFIVMVIVINAITLGLETSPTAMQQAGGL
                     LLALDRAALAVFVVELALKLYVYRLRFFRSGWNVFDFTIVAITLAPTGEGLQVLRSLR
                     ILRALRLVSVVPSMRKVVNALLRAIPGMSSVLTLLLLVFYVAAVMSTKLFGASFPEWF
                     GSIGESFYTLFQVMTLESWSMGIARPVMEAYPYAWIFFVLFILLTTFAVLNLFIAIVV
                     DSMSAVEHEEQEQTRELVGVDHDAVVAELRAIRAELAALRGAGGEPPPTA"
     gene            complement(853..1257)
                     /locus_tag="PKV97_13075"
     CDS             complement(853..1257)
                     /locus_tag="PKV97_13075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNB06858.1"
                     /translation="MRRLLSLVALLPLAAAIAAGPQTTTLPSGVTVETYRVGETGAPP
                     TLADVVRVHYQGTLADGKEFDSSYSRGRPAELPLEALVPCWKEVLQQMRPGGAVRIVC
                     PPETAYGSRGVPGRIPPDSTLNFVIELLEVKR"
     gene            complement(1274..1660)
                     /locus_tag="PKV97_13080"
     CDS             complement(1274..1660)
                     /locus_tag="PKV97_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNB06859.1"
                     /translation="MNLLLDTHVALWAITDHPRLPQAARDLILSPKTTVWVSVASVWE
                     IAIKHALGRGDMPVSSRDAVRYFTASGYRLLAIEPEHVVAVETLPAHHQDPFDRLLVA
                     QALVEPMRLVTHDRFVALYSDTIIKI"
     gene            complement(1663..1905)
                     /locus_tag="PKV97_13085"
     CDS             complement(1663..1905)
                     /locus_tag="PKV97_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNB06860.1"
                     /translation="MQSVNMLQAKSSLSRLVEAIESGREREIVIARNGRPAARLVPME
                     SASMGPRIGVAKGRFEVPDDIDASNEDVARLFLAGG"
     gene            complement(2112..>4132)
                     /gene="alaS"
                     /locus_tag="PKV97_13090"
     CDS             complement(2112..>4132)
                     /gene="alaS"
                     /locus_tag="PKV97_13090"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNB06861.1"
                     /translation="DRYIEIWNNVFMQFNRDEQGVMHPLPKPSVDTGMGLERISAVLQ
                     HVHANYEIDLFQALIKAAARETCSADMDSPSLKVLADHIRACSFLIADGVIPGNEGRG
                     YVLRRIIRRAIRHGYKLGARAAFFHKMVPDLVAEMGSAYPELKDGERRIMDVLRQEEE
                     RFFATIENGMAIVEAELAAMEAAGKKVFDGDTAFKLHDTFGFPLDLTADICREREVTV
                     DALAFDAAMARQKEQARAAGKFKMAANLEYDGPETTFHGYTLLEEKGNILALYKDGVP
                     VNELLEGEMGVVVLDHTPFYAESGGQVGDRGELRAAAGIFGVEDTQKIQAAVFGHHGV
                     VRTGKLAVGQGVVARVDVAARAATARNHSVTHLMHKALREVLGAHVQQKGSLVDPDKT
                     RFDFAHSAPMSAEEIREVEEIVNREILANAATQAEVMALEAAQKSGAMMLFGEKYADE
                     VRVLSIGTSKELCGGTHVARTGDIGLFKIVGEGGVAAGIRRVEAITGENALRYAQEQE
                     RRVQGMSALLKVQPDEVAERVAGILDNVRALEKELARLKSKLAASQGDELVSQAVEIK
                     GIRVLAALLEGADAPTLRDTMDKLKDKLKSAAIVLAAVADGKVSLIAGVTADHTARVK
                     AGELVNFVAQQVGGKGGGRPDMAQAGGTQPENLAAALAGVKDWVAGKL"
BASE COUNT          600 a         1452 c         1357 g          723 t
ORIGIN      
        1 accgcccccc tacctccccc ctacccgaca tgaccgtcac cctgcaacgc ctcgtcgaat
       61 ccgccgcctt ccagcgcttc atcgtcatgg tgatcgtcat caacgcgatc accctcggcc
      121 tcgagacctc gcccaccgcc atgcaacagg cgggcggact gctgctcgcg ctcgaccgcg
      181 ccgcgctcgc ggtgttcgtg gtcgagctcg cgctcaagct ctacgtctac cgcctgcgct
      241 tcttccgcag cggctggaac gtgttcgact tcaccatcgt cgcgatcacc ctcgccccca
      301 ccggcgaagg cctgcaggtg ctgcgctcct tgcgcatcct gcgcgcgctg cgcctggtgt
      361 cggtggtgcc ctcgatgcgc aaggtcgtca atgcgctgct gcgcgccatc cccggcatga
      421 gctcggtgct caccctgctg ctgctcgtgt tctacgtcgc cgcggtgatg agcaccaagc
      481 tcttcggcgc gagcttcccg gagtggttcg gaagcatcgg cgaatccttc tacaccctgt
      541 tccaggtcat gaccctggag tcgtggtcga tgggcatcgc ccgcccggtc atggaggcct
      601 atccctacgc gtggatcttc ttcgtcctct tcatcctgct caccaccttc gccgtgctca
      661 acctcttcat cgccatcgtg gtcgattcga tgagcgcggt cgaacacgag gagcaggagc
      721 agacccgcga gctggtcggc gtggaccacg acgcggtggt cgccgaactg cgcgcgatcc
      781 gcgccgagct ggccgcgctg cggggggccg gtggcgagcc cccacccacc gcctgacgcg
      841 gcaccgaccg cctcagcgct tgacctcgag cagctcgatc acgaagttca gcgtgctgtc
      901 gggcgggatg cggccgggca cgccgcgact gccgtaggcg gtctccggag gacatacgat
      961 gcgcaccgcg ccgcccggtc tcatctgctg cagcacttcc ttccagcacg gcaccagcgc
     1021 ctccagcggc agctcggccg ggcggccgcg gctgtaggag ctgtcgaact ccttgccgtc
     1081 ggcgagcgtg ccctggtaat gcacccgtac cacgtcggcc agcgtcggcg gcgcaccggt
     1141 ctcgcccacg cggtaggtct cgacggtgac gcccgagggc aaggtcgtgg tctgcggccc
     1201 ggcggcgatc gcagcggcaa ggggaagcag ggcgacgagg gaaagcagtc ggcgcatgtc
     1261 ggactccgga gattcagatc ttgatgatgg tgtcgctgta gagcgccacg aaacgatcgt
     1321 gcgtgaccag gcgcatcggc tcgaccagtg cttgtgccac cagaagacgg tcgaaagggt
     1381 cctgatgatg cgcgggcaag gtctcgaccg ccacgacgtg ctcgggctcg atcgccagga
     1441 ggcgataccc cgaggcggtg aaatagcgca cggcatcccg gctggacacc ggcatgtctc
     1501 cacgcccgag cgcatgcttg atggcgatct cccagacgct tgcgacgctg acccataccg
     1561 tcgttttggg cgacaggatc agatcgcgcg cggcctgcgg cagcctgggg tggtcggtga
     1621 tggcccacag cgcgacatgc gtatccagaa gcagattcaa gatcagcctc cggcgaggaa
     1681 cagcctggcg acgtcctcgt tgctggcgtc gatgtcgtcg ggcacctcga acctgccctt
     1741 ggccaccccg atgcgcggcc ccatggacgc gctctccatc ggaacgagcc gcgcggcagg
     1801 gcggccgttg cgggcgatga cgatctcgcg ctcccggccg gactcgatcg cctccaccag
     1861 gcgggacaag gatgactttg cctgcagcat atttacggat tgcatggagg cctcctcgga
     1921 cacttgttta gtctagtctg gctaatatgt tcctgcaaac gagttcgcgg gattctccat
     1981 tgcaccgacg gcaccgcctg cgccggagcc cgtctcgaaa aacgacgccc ggcaatcgcc
     2041 gggcgtcgtc ctgcatgcaa agccggatca caaacgagcg ccgctcgcgc tcgccgcgct
     2101 gaagatcgcc cttacagctt cccggccacc cagtccttca cccccgccag cgccgccgcc
     2161 aggttctccg gctgggtgcc gccggcctgg gccatgtcgg ggcggccgcc gcccttgccg
     2221 ccgacctgct gggcgacgaa gttgaccagc tcgccggcct tgaccctggc ggtgtggtcg
     2281 gcggtgacgc ccgcgatcag gctgaccttg ccgtcggcga ccgcggcgag cacgatcgcg
     2341 gccgacttga gcttgtcctt gagcttgtcc atggtgtcgc gcagggtcgg cgcgtcggcg
     2401 ccttcgagca gggcggcgag caccctgatg cctttgatct cgaccgcctg actcaccagc
     2461 tcgtcgccct ggctggccgc gagcttgctc ttgaggcggg ccagttcctt ctccagcgca
     2521 cgaacgttgt cgaggatgcc ggcgacgcgc tcggcgacct cgtcgggctg gaccttgagc
     2581 agcgccgaca tgccctgcac gcggcgctcc tgctcctggg cgtagcgcag cgcgttctcg
     2641 ccggtgatcg cctcgacgcg gcggatgccg gcggcgacac cgccctctcc gacgatcttg
     2701 aacaggccga tgtcgccggt gcgcgccacg tgggtgccgc cgcacagctc cttcgacgtg
     2761 ccgatcgaca gcacacgcac ctcgtcggcg tacttctcgc cgaacagcat catcgcgccc
     2821 gacttctgcg ccgcctccag cgccatcacc tcggcctgcg tggcggcgtt ggcgaggatc
     2881 tcgcggttga cgatctcctc gacctcgcgg atctcctcgg cgctcatcgg cgcggagtgg
     2941 gcgaagtcga agcgggtctt gtcgggatcg accagcgagc ccttctgctg cacgtgggcg
     3001 ccaagcactt cccgcagcgc cttgtgcatc aggtgggtga ccgagtggtt gcgcgcggtg
     3061 gcggcgcgcg cggcgacgtc cacccgcgcg accacgccct gcccgaccgc cagcttgccg
     3121 gtgcgcacca cgccgtgatg gccgaacacc gcggcctgga tcttctgcgt gtcttccacg
     3181 ccgaagatgc cggcggcagc gcgcagctcg ccgcggtcgc cgacctggcc gccggactcg
     3241 gcatagaacg gggtgtggtc gagcacgacc acacccatct cgccctcgag cagctcattg
     3301 accgggacgc cgtccttgta cagcgcgagg atgttgccct tctcttccag cagcgtgtag
     3361 ccgtggaagg tggtctcggg gccgtcgtat tccaggttgg cggccatctt gaacttgccg
     3421 gcggcgcgcg cctgctcctt ctggcgcgcc atcgcggcat cgaaggcgag cgcgtcgacg
     3481 gtgacctcgc gttcgcggca gatgtcggcg gtgaggtcga gcgggaagcc gaaggtgtcg
     3541 tgcagcttga aggcggtgtc gccgtcgaag accttcttgc ccgctgcttc catcgcggcg
     3601 agctcggcct cgacgatcgc catgccgttc tcgatggtgg cgaagaagcg ctcttcttcc
     3661 tggcgcagca cgtccatgat gcggcgctcg ccgtccttga gctcgggata ggcgctgccc
     3721 atctcggcga ccaggtcggg caccatcttg tggaaaaagg cggcgcgcgc gccgagcttg
     3781 tagccgtggc ggatggcgcg gcggatgatg cggcgcagca cgtagccgcg accctcgttg
     3841 cccgggatca cgccgtcggc gatcaggaag gagcaggcgc ggatgtggtc ggccagcacc
     3901 ttcagcgaag gcgagtccat gtcggcactg caggtttccc gcgccgccgc cttgatcagc
     3961 gcctggaaga ggtcgatctc gtagttggcg tgcacgtgct gcagcaccgc cgagatgcgc
     4021 tccaggccca tgccggtgtc caccgagggc ttgggcagcg gatgcatcac gccctgctcg
     4081 tcgcggttga actgcatgaa cacgttgttc cagatctcga tgtagcggtc gc
//
LOCUS       DAOQNN010000288         4125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13826_length_4125_cov_9.083784, whole genome shotgun sequence.
ACCESSION   DAOQNN010000288 DAOQNN010000000
VERSION     DAOQNN010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13826_length_4125_cov_9.083784"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKV97_13095"
     CDS             <1..253
                     /locus_tag="PKV97_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNB06862.1"
                     /translation="MLGGRLARAATRLLEARSQRLDALAAHLQHLAPQAVLARGYAIA
                     RDKQGRVLRSAAGIPEGTAVSVQLADGRLDTRVIGHGKA"
     gene            390..974
                     /locus_tag="PKV97_13100"
     CDS             390..974
                     /locus_tag="PKV97_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNB06863.1"
                     /translation="MEHTLPALPYAKDALAPHISAETLEFHYGKHHQAYVTNLNNLIK
                     GTEYENLDLEAIVKKAPAGGVYNNSAQVWNHTFFWNSMKPNGGGEPSGALADAIKAKW
                     GSFDDFKKAFQASAVGNFGSAWTWLVKKADGSVDIVNMGAAGTPLTTGDKALLCIDVW
                     EHAYYIDYRNRRPDFVATFLDKLANWDFAAKNFA"
     gene            1117..1674
                     /locus_tag="PKV97_13105"
                     /pseudo
     CDS             1117..1674
                     /locus_tag="PKV97_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            1987..3063
                     /gene="tal"
                     /locus_tag="PKV97_13110"
     CDS             1987..3063
                     /gene="tal"
                     /locus_tag="PKV97_13110"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNB06864.1"
                     /translation="MNPLLQVRQHGQQIWLDNLSRTLLEEGHLARFVADDGVAGVTTN
                     PAIFHKAISGGRYYEDDLAALKQQPLSAEARYEALVIPDVQRACDLLAPLHRDSGGSA
                     GYVSLEVSPALAHDADGTVAAGLRLKAAVDRPNLLIKVPATPAGLVAIERLIGEGVSV
                     NVTLMFSLAHCEGVAEAYLRGLARLRAAGGDVAGVMSVASLFLSRVDTLVDKLLEERG
                     GDLPALRGRTAVAMARLAYEAYQERFHGAGFEDLAAAGARPQYMLWASTGTKNPAYSD
                     LLYVEPLIGAETINTLPDATLDALRDHGHVASTLEQDVEQAAAHFTALAAAGIDLVAV
                     GERLQQDGLAQFEQAFAGLLELTA"
     gene            complement(3106..>4125)
                     /locus_tag="PKV97_13115"
     CDS             complement(3106..>4125)
                     /locus_tag="PKV97_13115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNB06865.1"
                     /translation="QNAVMKTRMQPIGRLFHKYPRIARDLARNLGKEVELALVGEDTE
                     IDKTMVEDLSDPIIHLIRNAVDHGIEDPARRAAAGKPAKSVLRLEARQEGDHIVITVA
                     DDGRGMDPERLRAKALAQGVITAEEASTLDERQSYDLIFLPGFSTAEKVSDVSGRGVG
                     MDVVRTNIQKLNGSIEIQSQLGKGTTLLIHLPLTLAILPVLLVRLGDQPFAVPLSMVR
                     EILPIEPGGIQDVGGKATMVVRGEVLPIVPLSALLGWPREAVPQYGVVMQSGAAVFIL
                     AIDSFAGREDAVIKSLEVFRPKGVAGVTTLANGQIVLILDMKELLESAGDRRSVSRST
                     LFEAA"
BASE COUNT          651 a         1540 c         1354 g          580 t
ORIGIN      
        1 catgctcggt gggcgcctcg cgcgcgcagc cacccgcctg ctggaagccc gcagccagcg
       61 cctcgacgcc ctcgccgccc acctccagca tctcgcgcca caggcggtac tggcgcgcgg
      121 ctatgccatc gcccgtgaca agcaaggccg cgtcctgcgc agcgccgccg gcatccccga
      181 gggcaccgcg gtgagcgtgc agctcgcgga cggccgcctc gacacccggg tcatcggcca
      241 cgggaaggcc tgagcgcacg ggttccgcaa atcccccgca tgggcttacc atgcgggaag
      301 caagcttgtt cggggtctaa atccagacta aaatggtccg gcttctaccc ccaacccgaa
      361 cacagacaga ccaacaacgg agatcacgca tggaacacac cctgcccgcc ctgccctatg
      421 ccaaggacgc cctcgccccg cacatctcgg ccgagaccct ggagttccac tacggcaagc
      481 accaccaggc ctacgtcacc aacctgaaca acctgatcaa gggcaccgag tacgagaacc
      541 tcgacctcga agccatcgtc aagaaggccc ccgccggcgg cgtgtataac aactccgcgc
      601 aggtgtggaa ccacaccttc ttctggaaca gcatgaagcc caacggcggc ggcgagccca
      661 gcggcgcgct ggccgacgcg atcaaggcca agtggggctc cttcgacgac ttcaagaagg
      721 ccttccaggc ctccgcggtc ggcaacttcg gttccgcctg gacctggctg gtcaagaagg
      781 ccgacggctc ggtcgacatc gtcaacatgg gcgccgccgg cacccccctg accaccggcg
      841 acaaggccct gctctgtatc gacgtgtggg aacacgccta ctacatcgac taccgcaacc
      901 gtcgcccgga cttcgtcgcc accttcctcg acaagctggc caactgggac ttcgcggcga
      961 agaacttcgc ctgattcccg gagccggccc gcggcgggcc gagctggatg cgaaagcgac
     1021 ccgcgtggtc gctttcgtgc tttatggccg gtacgagcgt cggcgcgcgc gcgcagcccc
     1081 ctgcgcgtcg gcgaggcgcc accgacgaga cacgagatgc tcgagatcct ctaccgcgac
     1141 gactggctgg ttgcgatcca caagccctcc ggcctgctcg tgcaccgcag cccgatcgcc
     1201 gcgcacgagg aacgcttcgc ggtgcagctg ctgcgcgacc agctcggccg ccgcgtgtat
     1261 cccgcccacc ggctggaccg cggcacctca ggcgtgctgc tgttcgcact cgatcgcgag
     1321 gtggcgcgca cgctcgcgca gcgtttcgag gcgcaggcgg tcgacaagcg ctacctggcg
     1381 gtcgtgcgcg ggcatccgcc cgagcacggc gtgatcgagc acgcgctggt gcgccgactc
     1441 gatgcggtcg aagtgcgcag cggcaagggc gccggggcgc gcgacgcgct gcccgaggat
     1501 gtcgacgaca gtgattcgcc agaggctgcc gagccggtcg cccagcccgc gcgcacccgc
     1561 ttccgccgcc tcgccacggt cgagctgccg catgcggtgg accgctaccc cagcagccgc
     1621 tacgcgctcg tcgagctctt ccccgagacc gggcgccgcc atcagctgcg ccgccacctc
     1681 aagcacatcg cccacccgat catcggcgac gccacctacg gcaagggccg ccacaaccgc
     1741 ctgttccagg cgctcttcgg cagccaccgc ctgctgctcg catgcacgcg gctagcgctg
     1801 gcgcatccga cgacggagcg cctgctggag atcgaggcgc cggtggcgga ggattttgcc
     1861 gcggtgctcg cggcgctcgg ctggcagacg gcacagcgca gcactttcga ggcagaatcc
     1921 aggctttccg ccgcccgcgg gcccgcgccc gagcggcccc gttcccacga cactccaggt
     1981 agaacgatga acccgctgct ccaggtacgc cagcacggcc agcagatctg gcttgacaac
     2041 ctctcccgca ccctgctcga ggaaggccac ctcgcccgct tcgtcgccga cgacggcgtc
     2101 gccggcgtga ccaccaaccc ggcgatcttc cacaaggcga tttccggcgg gcgttattac
     2161 gaggacgatc tcgccgcgct caagcagcag ccgctgagcg ccgaggcgcg ctacgaggcc
     2221 ctggtgatcc ccgacgtgca gcgcgcctgc gacctgctcg cacccctgca tcgggacagc
     2281 ggcggcagcg ccggctacgt cagcctggaa gtctcgcccg cgctcgccca cgacgccgac
     2341 ggtaccgtgg ccgccggcct gcgcctgaag gccgcggtcg accgccccaa cctgctgatc
     2401 aaggtgcccg ccacgccggc gggcctggtc gccatcgagc gcctgatcgg cgagggcgtc
     2461 agcgtcaacg tcacgctgat gttctcgctc gcgcactgcg agggggtggc cgaggcctac
     2521 ctgcgcgggc tcgcgcgtct gcgcgcggcg ggcggagacg tcgcgggcgt gatgtcggtg
     2581 gcgagcctgt tcctgtcgcg ggtcgacacc ctggtggaca agctgctgga agagcgcggc
     2641 ggcgacctgc cggcgctgcg cggccgcacc gcggtggcga tggcgcggct ggcctacgag
     2701 gcctaccagg agcgcttcca cggcgccggc ttcgaggacc tggccgcggc gggcgcacgt
     2761 ccgcagtaca tgttgtgggc aagcaccggc accaagaacc cggcctacag cgacctgctc
     2821 tacgtcgagc cgctgatcgg tgccgagacc atcaacaccc tgcccgacgc cacgctcgac
     2881 gccctgcgcg accacggcca cgtggcttcg acgctggaac aggacgtcga gcaggcggcc
     2941 gcccacttca cggccctcgc agcagccggc atcgacctcg tggccgtggg cgagcgcctg
     3001 cagcaggacg gcctggcgca gttcgagcag gccttcgcag ggctgctcga actcaccgcc
     3061 tgagttgcca cctcgctcgc cagccgagcg cgaccggggc ggcggctacg ccgcctcgaa
     3121 cagcgtcgag cgcgacacgc tgcgccgatc gccggccgac tcgagcagct ccttcatgtc
     3181 gaggatcagc acgatctggc cgttggcgag cgtggtcacc ccggccacgc ccttcggccg
     3241 gaagacctcg agcgacttga tcaccgcgtc ctcgcgcccg gcgaagctgt cgatcgcgag
     3301 aatgaacacc gcggcgcccg actgcatcac cacgccatac tgcggcaccg cctcgcgcgg
     3361 ccagccgagc agcgcggaca gcggcacgat cggcagcacc tcgccgcgca ccaccatggt
     3421 ggccttgcca ccgacgtcct ggatgccgcc cggctcgatc ggcaggatct cgcgcaccat
     3481 cgacagcggc acggcgaagg gctgatcgcc gaggcgcacc agcagcaccg gcaggatcgc
     3541 cagggtcagc ggcaggtgga tcagcagcgt ggtgcccttg ccgagctggg actggatctc
     3601 gatgctgccg ttgagtttct ggatgttggt gcgcaccacg tccatgccga cgccgcggcc
     3661 ggacacgtcc gacaccttct cggcggtgga aaacccgggc aggaagatga ggtcgtagct
     3721 ctggcgctca tcgagcgtgc tcgcctcctc ggcggtgatc acgccttgcg cgagcgcctt
     3781 cgcgcgcagc ctctcgggat ccatgccgcg gccgtcgtcg gccacggtga tcacgatgtg
     3841 gtcgccctcc tggcgcgcct ccaggcgcag caccgacttg gccggcttgc cggcggcggc
     3901 gcgccgggcg ggatcctcga tgccgtgatc caccgcgttg cggatgaggt ggatgatcgg
     3961 gtcggagaga tcctcgacca tggtcttgtc gatctcggtg tcctcgccga ccagcgccag
     4021 ctcgacctcc ttgccgaggt tgcgcgcgag gtcgcgggcg atgcgcgggt acttgtggaa
     4081 gagccggccg atcggctgca tgcgcgtctt catgaccgcg ttctg
//
LOCUS       DAOQNN010000289         4111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13894_length_4111_cov_10.063609, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000289 DAOQNN010000000
VERSION     DAOQNN010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13894_length_4111_cov_10.063609"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..98
                     /locus_tag="PKV97_13120"
     CDS             <1..98
                     /locus_tag="PKV97_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HNB06866.1"
                     /translation="ARARKYLAEQMEKHFFEGGADQVAGYVPPQA"
     gene            complement(159..851)
                     /gene="phoU"
                     /locus_tag="PKV97_13125"
     CDS             complement(159..851)
                     /gene="phoU"
                     /locus_tag="PKV97_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNB06867.1"
                     /translation="MTEHTLRQYDIELEEIRRGVLNMGGLAEAQVVKAIEGLRSGQLD
                     LLDTVIEGDKPINLAQVELDDDCSHIIAKRQPAAGDLRLVLAVIKIASDLERIGDEAK
                     KIAKAARRLHSAETPFVPRVGLTQAVELALELLRASLDAFARVNPEGIEDLRRKDAEI
                     DAGFKGVMRQLITYMMEDPRTISSCLEMLFIAKAIERVGDHAMNIAEHVVFVARGEDV
                     RFEKSQAQLGRG"
     gene            complement(941..1603)
                     /gene="rpiA"
                     /locus_tag="PKV97_13130"
     CDS             complement(941..1603)
                     /gene="rpiA"
                     /locus_tag="PKV97_13130"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNB06868.1"
                     /translation="MTQDELKKAAAYAALDHVVEGSIVGVGTGSTVNHFIDALAGMRD
                     RIRGAVSSSEASTQRLRAHGIEVFDLDGIESLPVYVDGADEIDGGFAMIKGGGGALTR
                     EKIVAAVSERFVCICDASKKVEVLGRFPLPIEVIPMARSHVARELRALGGEPRLREGF
                     VTDNGNLILDVHGLSITDPVAIEMRINQIVGVVTNGLFAVRGADVLLLAAPGGVQRLD
                     AR"
     gene            complement(1660..2907)
                     /locus_tag="PKV97_13135"
     CDS             complement(1660..2907)
                     /locus_tag="PKV97_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNB06869.1"
                     /translation="MNPNPAGGDDRSFLAVDAARAAILGALTPIAGRERVAVRAALGR
                     VLAEDVIAPCNVPAHDNSAMDGYAVRAADLSADGESALAVVGTAFAGRPFSGMVGAGQ
                     AVRIMTGATIPQGADTIVVQEVARREHERVFIPIGQKKGQNLRRAGEDLAEGGVAIPA
                     GKRCGPAELGLIASLGIAEVCVRRRLRVAVMSTGDEIASIGRPLGPGEVYDSNRYTLH
                     GVLTRLGCELLDLGVVPDRPEALEEAFREAAQAADVIITSGGVSVGEADFIREMMNRM
                     GEVAFWKLAIKPGRPMAFGRIGDAVLFGLPGNPVAVMVTFYQFVQDALLQLMGVAPLP
                     APERFPVRAAFSMRKQPGRVEYLRGRLQRSGEALAVAPAGAQGSGVLSSMSEADCFVV
                     LPADCTAVREGDTVLVQPFQGIV"
     gene            complement(2957..>4111)
                     /gene="ccsB"
                     /locus_tag="PKV97_13140"
     CDS             complement(2957..>4111)
                     /gene="ccsB"
                     /locus_tag="PKV97_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HNB06870.1"
                     /translation="TPAGPAPAALRPHTPLLRRLGAFDWLYALALMLGAAFALVRYHA
                     VMDVYDDLILVLQVPVLAVIGWQWKPFRAFLAAVALLALASIALYQGDLARAEQTFLL
                     KYLISSQTAIMWMSALIFIATGAYWLGLLARSDFAERTGSALTWIAAVMGATGLFVRW
                     YESYLLGPEIGHIPISNLYEVFVLFALMTALLYLYYEGRYATRRMGAFVMIVISAAVA
                     FLLWYTFTREAHEIQPLIPALQSYWMKVHVPTNFIGYGAFAVSAMVGTAYLLAERGIL
                     ASRLPPLRVLEDVMYKAIAIGFAFFTIATILGALWAAEAWGTYWQWDPKETWALIVWL
                     NYATWLHMRLTKGLRGAVLAWWAVVGLLVTTFAFIGVNMFLTGLHSYGAL"
BASE COUNT          646 a         1365 c         1497 g          603 t
ORIGIN      
        1 acgcgcgcgc gcgcaagtac ctcgccgagc agatggagaa gcacttcttc gaaggtggcg
       61 ccgaccaggt ggccggctac gtcccgccgc aggcgtaagc ccggatcgcc gatgtaaaaa
      121 aggggagccg caagctcccc tttttcatgc ctgacgcctc agcccctgcc gagctgcgcc
      181 tgcgacttct cgaagcgcac gtcctcgccg cgcgccacga acaccacgtg ctcggcgatg
      241 ttcatcgcgt ggtcgccgac gcgctcgatc gccttggcga tgaagagcat ctcgaggcag
      301 ctcgagatcg tgcgcgggtc ttccatcatg taggtgatga gctggcgcat cacgcccttg
      361 aagccggcgt cgatctccgc gtccttgcgg cgcaggtcct cgatgccttc cggattcacc
      421 cgcgcgaagg cgtcgagcga ggcgcgcagc agctcgagcg cgagctccac cgcctgggtg
      481 aggcccacgc gcggcacgaa gggggtttcg gcgctgtgca ggcggcgcgc cgccttggcg
      541 atcttcttcg cctcgtcgcc gatgcgctcg aggtcggagg cgatcttgat caccgccagc
      601 accaggcgca ggtcgccggc ggccggctgg cgcttggcga tgatgtggct gcagtcgtcg
      661 tcgagctcga cctgcgcgag gttgatcggc ttgtcgcctt cgatcacggt gtcgagcagg
      721 tcgagctggc cgctgcgcag gccctcgatc gccttgacga cctgcgcctc cgccaggccg
      781 cccatgttga ggacgccacg gcggatttct tcgagttcga tgtcgtactg tctgagagtg
      841 tgttctgtca tgttcgccac gatccagtcc gggaagcgcg caggctaaca ggcgattgtt
      901 aaggttttgt tgcctgcaac acggctgcca tgcaggtcgc tcaccgtgcg tccagccgtt
      961 gcacgccgcc gggggcggcg agcagcagca cgtcggcgcc gcgcacggcg aagagcccgt
     1021 tggtgactac accgacgatc tggttgatcc gcatctcgat ggcgacgggg tcggtgatcg
     1081 acagcccatg cacgtcgagg atcaggttgc cgttgtcggt gacgaagccc tcgcgcagcc
     1141 tgggctcgcc gccgagcgcg cgcagctcgc gggcgacgtg gctgcgggcc atcgggatca
     1201 cctcgatcgg cagcgggaag cggccgagca cctcgacctt cttgctcgcg tcgcagatgc
     1261 acacgaagcg ctcggacact gcggcgacga tcttctcgcg ggtcagtgcg ccgccgccgc
     1321 ccttgatcat cgcgaagccg ccgtcgatct cgtcggcgcc gtctacatac accggcagcg
     1381 actcgatgcc gtcgaggtcg aacacctcga tgccgtgcgc gcgcaggcgt tgcgtgctcg
     1441 cttcggagct ggagaccgcg ccgcggatgc gatcgcgcat gccggcgagc gcatcgatga
     1501 agtggttgac ggtggagccg gtgccgacgc cgacgatgct gccttcgacc acgtggtcga
     1561 gagcggcgta ggcggcggct ttcttgagtt cgtcctgggt catgtgcgcg aggaagggat
     1621 gaggtgggaa ggggaaaggg ggagggggaa gccggccgct cagacgatgc cctggaaggg
     1681 ttgcacgagg acggtgtcgc cttctctcac ggcggtgcag tcggccggca gcacgacgaa
     1741 gcagtcggcc tcggacatgg agctcagcac gccggagccc tgggcgccgg cgggcgccac
     1801 ggcaagcgcc tcgccgctgc gctgcaggcg gccgcgcagg tactcgacgc gacccggctg
     1861 cttgcgcatc gagaaggcgg cgcgcacggg gaagcgctcg ggcgcgggca gcggtgcgac
     1921 gcccatgagc tggagcagcg cgtcctgcac gaactggtag aaggtgacca tcaccgccac
     1981 cgggttaccg ggcagcccga acagcaccgc atcgccgatg cggccgaagg ccatcggccg
     2041 cccgggcttg atcgcgagct tccagaacgc cacctccccc atgcggttca tcatctcgcg
     2101 gatgaagtcg gcctcgccga ccgacacgcc gccgctggtg atgatcacgt cggcggcctg
     2161 ggcggcctcg cggaaggcct cctcgagcgc ctccgggcgg tccggcacga cgccgaggtc
     2221 gaggagctcg cagcccaggc gggtgagcac gccgtgcagg gtgtagcggt tgctgtcgta
     2281 gacctcgccg gggccgagcg ggcggccgat cgaggcgatc tcgtcgccgg tggacatcac
     2341 cgccacgcgc aggcggcggc gcacgcacac ctcggcgatg ccgagcgagg cgatcaggcc
     2401 gagttcggcg ggaccgcagc gcttgcccgc ggggatcgcg accccgccct cggcgaggtc
     2461 ctcgccggcg cggcgcaggt tctggccctt cttctgcccg atggggatga acacgcgctc
     2521 gtgctcgcga cgggcgactt cctgcacgac gatggtgtcg gcgccctgag gtatggtggc
     2581 gccggtcatg atgcgcacgg cctggccggc gccgaccatg cccgagaagg gccggcctgc
     2641 gaaggcggtg ccgaccaccg cgagcgcgct ctcgccatcg gccgagaggt cggcggcgcg
     2701 caccgcgtag ccgtccatcg cggagttgtc gtgcgcgggc acgttgcacg gcgcgatcac
     2761 gtcctcggcg agcacgcggc cgagcgcggc gcgtaccgcg acgcgctcgc gcccggcgat
     2821 cggggtgagc gcgccgagga tcgcggcgcg cgcggcatcg acggcgagga agctgcggtc
     2881 gtcgccgcct gcgggattgg gattcatgct tcgggtccgt cctcgatggg cgaccgtccg
     2941 tttcgctgcg gcgcgctcac agcgcaccgt aggagtgcag gccggtcagg aacatgttga
     3001 cgccgatgaa ggcgaaggtg gtgacgagca ggcccaccac cgcccaccat gccagcaccg
     3061 cgccgcgcag gcccttggtc aggcgcatgt gcagccaggt cgcgtagttg agccacacga
     3121 tcagcgccca ggtctccttc gggtcccact gccagtaggt gccccaggcc tcggccgccc
     3181 acagcgcgcc gaggatggtg gcgatggtga agaaggcgaa gccgatcgcg atcgccttgt
     3241 acatcacgtc ctcgagcacg cgcaagggcg gcaggcggga ggcgaggatg ccgcgctcgg
     3301 cgagcaggta ggcggtgccg accatggccg agaccgcgaa ggcgccgtag ccgatgaagt
     3361 tggtcggcac gtgcaccttc atccagtagg actgcagcgc cgggatcagc ggctggatct
     3421 cgtgcgcctc gcgggtgaag gtgtaccaga gcaggaaggc caccgcggcc gagatcacga
     3481 tcatcacgaa ggcgcccatg cgccgcgtgg cgtagcgacc ctcgtagtag aggtagagca
     3541 gcgccgtcat cagcgcgaac agcacgaaga cctcgtacag gttgctgatc gggatgtggc
     3601 cgatctcggg gccgagcagg taggactcgt accagcgcac gaagaggccg gtggcgccca
     3661 tgaccgcggc gatccaggtc agcgccgagc cggtgcgctc ggcgaagtcg ctgcgtgcga
     3721 gcaggcccag ccagtacgcg cccgtggcga tgaagatcag cgcgctcatc cacatgatcg
     3781 cggtctggct ggagatcagg tacttgagca ggaaggtctg ctcggcgcgc gcgagatcgc
     3841 cttggtagag cgcgatgctc gccagcgcca gcagcgccac cgcggcgagg aaggcccgga
     3901 agggcttcca ctgccagccg atcacggcga gcaccggcac ttgcagcacc aggatgagat
     3961 cgtcgtatac gtccatcacc gcgtggtagc ggacgagcgc gaaggccgcg cccagcatca
     4021 gcgcgagcgc gtacagccag tcgaaggcgc cgagccggcg cagcagcggc gtgtgcggcc
     4081 ggagcgcggc gggggccgga ccggccgggg t
//
LOCUS       DAOQNN010000290         4099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_13958_length_4099_cov_6.966370, whole genome shotgun sequence.
ACCESSION   DAOQNN010000290 DAOQNN010000000
VERSION     DAOQNN010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13958_length_4099_cov_6.966370"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /locus_tag="PKV97_13145"
     CDS             complement(<1..1727)
                     /locus_tag="PKV97_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNB06871.1"
                     /translation="MKPEKKPAAPAAEAPVVSNFIRSIIDEDNRNGKWGGRVETRFPP
                     EPNGYLHYGHAKSICLNFGLAESYGGVCHMRFDDTNPEKEEQEYVDAILEAVQWLGFE
                     WGEHLYFASDYFDKMYACAISLIKAGKAYVDSQSAEEMRVNRGTLTEAGKNSPYRERS
                     VDENLELFARMRAGEFAEGTHILRAKVDMASPNINLRDPAIYRIRHATHHRTGDAWHL
                     YPMYTFAHPIEDAIENITHSVCTLEFEDQRPFYDWLLDALATEGHFPRPLPQQIEFAR
                     LNLTYVVLSKRKLIQLVNEGHVDGWDDPRLPTLVGARRRGFTAAGFRRFAERIGVSKA
                     ESWIDMSVLEECMRDDLNEAAQRRVAVLDPLKLVLTNYAEGASELCQAPNHPLEPELG
                     KRDMPFSRELWIEREDFMEEPVKGFHRLYPGNMVRLRYGFVVKCTGCEKDADGKVTAV
                     LAEYMPDSKSGTPGADDYKVKGNLHWVSVAHGFETEVRIYDRLFAHPHPGQRREGDAP
                     DFERDFLDDINPDSKRVITAYLEPALKDARPEDRFQFERHGYFVADRVDSKPGAPVFN
                     RTVTLKDSWA"
     gene            1954..2337
                     /gene="crcB"
                     /locus_tag="PKV97_13150"
     CDS             1954..2337
                     /gene="crcB"
                     /locus_tag="PKV97_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNB06872.1"
                     /translation="MNLAAFAAVGAGAACGAWLRWGLGLWLNPVFALVPLGTLMANLL
                     GGFLMGVVLAAVQAWPTMSPALKLLLTTGLLGGLTTFSTFSAESFHLLQRGAIGWLGA
                     HLVVHVAGSVLMTWAGYVLFNALRA"
     gene            2472..3140
                     /locus_tag="PKV97_13155"
     CDS             2472..3140
                     /locus_tag="PKV97_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB06873.1"
                     /translation="MKKIIVTLSAVALAVSGLSGCANMNETQRDTGMGAAIGAVAGGL
                     IGAATAGGNKGKSAATGAAIGAALGAGGGYLWSQNMQKQRAEMEQATAGTGIGISQTT
                     DNQLKVDIPADVSFDVGRHAIKPNMRPVLDRLASTLNQHPVTTVTIIGHTDSTGSDAV
                     NDPLSINRAAATRDYLVQRGVSAQRIAVDGRGSRQPVADNSTASGRAMNRRVEIFIAE
                     PAQR"
     gene            complement(3368..>4099)
                     /locus_tag="PKV97_13160"
     CDS             complement(3368..>4099)
                     /locus_tag="PKV97_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HNB06874.1"
                     /translation="IVRSVGLEGGQRDLYETVRAAMDEKVRAEIAGKGFARSQIVILD
                     ALLKLRQVCCDPRLLKSPAALRVKERAKLDLLMDMLPELIDEGRRILVFSQFTTMLGL
                     IAGELDKAKIGWVALTGDTRDRRVPVEDFQKGRVPVFLISLKAGGVGLNLTAADTVIH
                     YDPWWNPAAENQATDRAHRIGQDKPVFVFKLVCAGSIEEKILALQERKAALAESVLSE
                     DADALAKFGEADIAALLAPLPADAR"
BASE COUNT          579 a         1317 c         1488 g          715 t
ORIGIN      
        1 gcccacgaat ccttcagcgt caccgtgcgg ttgaacaccg gcgcgccggg cttcgagtcc
       61 acgcggtcgg cgacgaagta gccgtggcgc tcgaactgga agcggtcttc gggccgggcg
      121 tccttcagtg ccggctccag gtaggcggtg atgacgcgct tgctgtcggg gttgatgtcg
      181 tcgaggaagt cgcgctcgaa gtcgggcgcg tcgccttcgc ggcgctggcc ggggtggggg
      241 tgggcgaaga ggcggtcgta gatgcgcacc tcggtttcaa aaccgtgcgc caccgacacc
      301 cagtgcaggt tgcccttgac cttgtagtcg tcggcgcccg gcgtgccgga cttcgagtcg
      361 ggcatgtact cggcgagcac cgcggtgacc ttgccgtcgg cgtccttctc gcagccggtg
      421 cacttgacga cgaagccgta gcgcaggcgc accatgttgc ccggatagag gcggtggaag
      481 cccttgacgg gctcttccat gaagtcctcg cgctcgatcc acagctcgcg cgagaagggc
      541 atgtcgcgct tgccgagctc gggctcgagc gggtggttgg gtgcctggca cagctcggag
      601 gcgccttcgg cgtagttggt aaggacgagc ttcagcgggt cgagcaccgc cacgcggcgc
      661 tgggcggcct cgttgagatc gtcgcgcatg cactcttcca gcacgctcat gtcgatccac
      721 gactcggcct tggacacgcc gatgcgctcg gcgaagcggc ggaagccggc ggcggtgaag
      781 ccgcggcggc gggcgccgac cagcgtgggc aggcgcgggt cgtcccagcc gtcgacatgg
      841 ccttcgttca ccagctggat cagcttgcgc ttggacagca ccacgtaggt caggttgagg
      901 cgcgcgaact cgatctgctg cggcagcggg cgcgggaagt ggccttcggt ggcgagcgcg
      961 tcgagcagcc agtcgtagaa cgggcgctgg tcctcgaact cgagcgtgca caccgagtgg
     1021 gtgatgttct cgatcgcgtc ctcgatcgga tgcgcgaagg tgtacatcgg gtacaggtgc
     1081 caagcgtcgc cggtgcgatg gtgggtggcg tggcggatgc ggtagatcgc cgggtcgcgc
     1141 aggttgatgt tggggctcgc catgtcgacc ttggcgcgca ggatgtgcgt gccctcggcg
     1201 aattcgccgg cgcgcatgcg cgcgaagagc tcgaggttct cgtcgacgct gcgctcgcgg
     1261 tacgggctgt tcttgcccgc ctcggtcagg gtgccgcggt tgacgcgcat ctcctcggcc
     1321 gactgcgaat cgacataggc cttgccggcc ttgatcaggc tgatggcgca ggcgtacatc
     1381 ttgtcgaagt agtccgacgc aaagtacagg tgctcgcccc actcgaagcc gagccactgc
     1441 accgcctcga ggatggcgtc gacatattcc tgctcttcct tctcggggtt ggtgtcgtcg
     1501 aagcgcatgt ggcagacgcc gccgtaggac tcggccaggc cgaagttcag gcagatcgac
     1561 ttggcgtggc cgtagtgcag gtagccgttg ggctcgggcg ggaagcgtgt ctcgacgcgg
     1621 ccgccccatt tgccgttgcg gttgtcctcg tcgatgatgc tgcggatgaa gttggagacg
     1681 acgggcgctt cggcggcggg tgcggcgggt ttcttttcgg gtttcatcgg gaaggcaggg
     1741 gtcggaaagt tcggcgggat ggtgggggtt cgtgtgcagc tggggccgga ccccgaggcg
     1801 ggcggcgcaa tggaccgatt gtagccgatc gggcgtgcgg gctcgccgga gcgggccggt
     1861 cacggggtgc ggcgttcggg gcgcgatgcc cgcggagggg tagaatcgtg cggccggtgg
     1921 gcggcagcag ggaggcggga tggagcacgg gcgatgaatc tggcggcatt cgcagcggtt
     1981 ggggcggggg cggcgtgcgg tgcgtggctg cgctggggat tggggctgtg gctcaacccg
     2041 gtgttcgcgc tcgtcccgct cggcaccctg atggcgaacc tgctgggcgg tttcctcatg
     2101 ggcgtagtgc tcgcggcggt gcaggcctgg ccgacgatgt cgccggcgct caagctcctg
     2161 ctcaccaccg gcctgctcgg cgggctgacg accttttcca ccttttccgc cgagtccttc
     2221 cacctgttgc agcgcggcgc gatcggctgg ctgggtgccc acctggtcgt gcatgtggct
     2281 ggatccgtgc tgatgacctg ggcgggatac gtgcttttca atgccctgcg tgcgtgacct
     2341 gcgccgcaag gcgggcggcc gcgtcatttt ctgtttcact ccgatgggct agagtggatg
     2401 agcgttacat cgataattca cgccgttctg catccccagt ggcaccccct caacgcaacg
     2461 gagcccatcc aatgaaaaaa atcatcgtca ccctttccgc ggtcgcgctc gccgtgagcg
     2521 gtctttccgg ctgcgcgaac atgaacgaga cccagcgcga cacgggcatg ggagccgcca
     2581 tcggcgccgt ggccggcggc ctcatcggtg ccgccaccgc gggcggcaac aagggcaaga
     2641 gcgccgcaac cggtgctgcg atcggcgctg cactcggggc gggcggcggc tacctgtggt
     2701 cgcagaacat gcagaagcag cgcgccgaga tggagcaggc gaccgcgggc accggcatcg
     2761 gcatcagcca gacgaccgac aaccagctca aggtcgacat tcccgcggac gtttccttcg
     2821 acgtgggccg ccacgcgatc aagcccaaca tgcgtccggt gctcgaccgt ctggccagca
     2881 cgctgaacca gcatccggtg accacggtga ccatcatcgg ccataccgac agcaccggct
     2941 cggatgcggt gaacgatccg ctatcgatca accgcgccgc cgccacccgc gattatctgg
     3001 tgcagcgtgg cgtgtccgcg cagcgcatcg cggtcgatgg gcgcggctcg cgccagcccg
     3061 tcgccgacaa ctcgacggcc tccggtcgtg cgatgaaccg ccgcgtggag atcttcatcg
     3121 cggagccggc gcagcgctga ccccggacgg ggtcgggtga gcgcttgcgg cgggatttcg
     3181 cgccgcgcca tctcgaccgc ttcaatcgcg actcgcgtcg ctcccacggg gctcctggag
     3241 ccccgtgctc attgcggggg cggcgcaagt ggcgactcgt gcgttcggtc gcttcggcgc
     3301 ggaggagcgc cggcaccgag gcttgcgccg ctcctgcagg acagctttgc ccggcttccg
     3361 ctcttgctca gcgcgcgtcc gcggggagcg gcgcgagcag ggcggcgatg tcggcctcgc
     3421 cgaacttggc gagcgcgtcc gcgtcctccg acagcacgct ctcggcgagg gcggccttgc
     3481 gctcctgcag ggcgaggatc ttctcctcga tgctgcccgc gcacaccagc ttgaacacga
     3541 acaccggctt gtcctggccg atgcggtggg cgcggtcggt ggcctggttc tcggccgcgg
     3601 ggttccacca cgggtcgtag tggatcacgg tgtcggcggc ggtgaggttg aggcccacgc
     3661 cgcccgcctt caggctgatc aggaataccg gcacgcgccc cttctggaag tcttccaccg
     3721 gcacccggcg atcgcgggtg tcgccggtca gcgcgaccca gccgatcttc gccttgtcga
     3781 gttcgccagc gatcaggccg agcatggtgg tgaactgcga gaacaccagg atgcgccggc
     3841 cctcgtcgat cagctcgggg agcatgtcca tgagcaggtc gagcttggcg cgctccttca
     3901 cccgcaaggc ggcgggcgac ttcaacaggc gcgggtcgca gcacacctgg cgcagcttca
     3961 gcagcgcgtc gaggatcacg atctggctgc gcgcgaagcc cttgccggcg atctccgcgc
     4021 gcaccttctc gtccatcgcc gcgcgcaccg tctcgtagag gtcgcgctgg ccgccctcca
     4081 ggccgacgct gcgcacgat
//
LOCUS       DAOQNN010000291         4079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14073_length_4079_cov_12.061133, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000291 DAOQNN010000000
VERSION     DAOQNN010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14073_length_4079_cov_12.061133"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4079)
                     /locus_tag="PKV97_13165"
     CDS             complement(<1..>4079)
                     /locus_tag="PKV97_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06875.1"
                     /translation="FTNGTGTGKTFLTLGAIKRLTKRGKGNGIIVVPNEAVMNEWVKS
                     APALGLQIKPLSDTQDAGSGIAITTYANFGQNDEIAKRDYDFVAIDEAHLLMLGKDAE
                     PTKALDALRAVSLHPDGVLQRAEMQNRELVDRISTTSDQLKSLYRMMNNPDIMDQMYA
                     AYRAEADGLEKQLAADRAKWKAVLDAVESDVMARQGEKRTRAIFLSATPFAYEPSIQW
                     GEGYLYEFPKVSGRGYNAPNGYQKFMMQHFGWRMRNGKLNKPPAEVDSDLMQRQFNTW
                     LRKEGVLSARMLDVDFDYERKFILIQGGVGQTIDDGLRWLRETDNGRYWSLYGAINEQ
                     FDYLTRSRLLEAIKARAAIPYIKKHHALGRKVVVFYDFNSGGGINVFNLSEQLKRAEA
                     AEKASDYSVGKDGVDVPVRGQGKVVHGQIKRENVPRSQLIREFMAARPELQAIDFSQY
                     GSPLTTLLEAFPGAGVYNGMSQYKKTRVQSIRDFNDDAKPEANLLLVQKAANAGWSGH
                     DTTGKHGRVLINLGLPVAPIEAIQEEGRIYRVGQKSDANLQYFNTGTNWERYAFGSKI
                     ARRAGTAENLAMGEQARGLREAFIQAFENSDPDYEPGAADGRGGKAGDRDLVGAITEW
                     DRARSLYFAQQKRTSRTKAAEGVDYYATPEPLGLKMVEWAGILTGESVLEPSAGHGAI
                     ARWFPEKSDRTVIEPSLELASRLTLATDAKLINERFEGHDTINKYDAIVMNPPFGTGG
                     KTAIDHLDKAFRHLTDGGRVVALIPEGPTANKRFEDWLYGDKQVKAKPVANDVGAFGA
                     VFMGDTVTVEVSGVAGIAGGVLGTGTITGKVTGFREGRLFVKRDGEALATGYRPDQIR
                     SVQPTGARTATVKSAPNAYLRASIGLPGVTFERAGTGVKTRVVIIDKIEGMRASRGVE
                     TQPEREIEAATVKELFDRLENMEVVGRSLPAHKEQERKQGQERSAAKDATAGKADDVG
                     LVQPDGSPATIEQLQKPEKAGGKTKLLTDAPRVKHVVARSGKELFGVIAKRLTRNEAK
                     AVDPYSWPKDGGFFIRMEHVVRPPSVVEQGTLPTSNAGGNVAFSAVSSGIAASPADRA
                     IYSMAAEGKSTDEILSFISKSSRRPFNRVLAAALRRAGLVSSITLDTASRFGKQQGAA
                     RYAAAYNPTTDTVALFTYRDAERHILHELVHAATMKAIAGKGPASVRMRALFRHVQKS
                     GRLDGQYGMTDVDEFVAEAFSNPRFQEALRGIPAPAGSTLKSAWQWFVRIVARIVGIK
                     TQPSETALDRALTVGAQLMNENAALAGRRGDGDTRYAIAWHGTPHVWPPEPGFPHGRP
                     RLDKMGTGEGAQAYGWGWYSAEARDVAASYKNYLSEPKI"
BASE COUNT          604 a         1402 c         1247 g          826 t
ORIGIN      
        1 caatctttgg ttcggaaaga tagttcttgt aactggccgc tacatcccgc gcttctgcag
       61 agtaccaacc ccatccgtaa gcctgcgccc cctcgccagt ccccatcttg tcgagccgcg
      121 gccggccgtg cgggaaccca ggctcaggag gccagacgtg cggggtgccg tgccacgcaa
      181 tcgcgtacct ggtgtcaccg tctcctctgc gcccagccag cgccgcattc tcgttcatca
      241 actgcgcccc gaccgtcagc gcacgatcga gcgccgtttc cgacggctgc gtcttgatgc
      301 cgacgatccg cgcgacgatc cgcacgaacc actgccaggc gctcttgagc gtcgatcctg
      361 ccggcgccgg gatgcctctc agcgcttcct gaaagcgtgg attgctgaag gcctcggcga
      421 cgaactcatc aacgtccgtc atcccgtact gcccgtcgag cctgccggac ttctggacat
      481 gccggaacag cgcccgcatg cggacagaag ccggcccttt gccggcgatc gccttcatgg
      541 ttgcggcatg caccagctcg tgcaggatgt ggcgctctgc gtcgcggtac gtgaacagag
      601 ccaccgtgtc ggtcgtcggg ttgtacgccg ctgcatagcg cgctgcgccc tgctgtttcc
      661 cgaagcgcga ggcggtgtcc agcgtgatgg acgacacgag gccagcccgg cgcagcgcag
      721 ccgccagcac gcggttgaac gggcgccggc tggacttgct gatgaacgac agaatctcat
      781 cagtcgactt gccttcggca gccatggagt agattgcgcg gtctgccgga gatgcggcga
      841 taccggacga aacagccgag aacgccacgt tgccgccagc gttcgaggtt ggcagcgtgc
      901 cctgctccac gacgctcggg gggcggacga catgctccat gcggatgaag aacccgccgt
      961 ccttcggcca tgagtatggg tcaacagcct tcgcttcgtt cctcgtcagc ctcttggcta
     1021 tgacgccgaa cagctctttc ccgctccttg cgacaacgtg cttgacgcgc ggcgcatccg
     1081 tgagaagctt cgtcttccct ccggccttct caggcttctg cagttgctcg atggttgcgg
     1141 gcgacccatc cggctgcacg agaccgacgt cgtccgcctt gccggcggtt gcgtccttcg
     1201 cggcgctgcg ctcctgcccc tgcttcctct cctgctcctt gtgtgccggc aaggaccggc
     1261 cgacaacctc catgttctcc aggcggtcga acagctcctt cacggttgcg gcttcgatct
     1321 cgcgctcggg ctgcgtctcg acaccccgag aggcgcgcat gccttcgatc ttgtcgatga
     1381 tcaccacgcg ggtcttgacg ccggtaccgg cgcgctcgaa cgtcaccccg ggaagtccga
     1441 tggacgcccg caggtacgca ttcggagcgc tcttgaccgt ggccgtgcgc gcccctgtcg
     1501 gctgcacgga cctgatctga tccgggcggt atccggttgc caaggcctct ccatcgcgct
     1561 tcacgaacag cctgccttcc cggaagccgg tcaccttgcc ggtgatcgtt ccggtgccca
     1621 ggacgccacc ggcaatgcca gcaactccgc tcacctcgac ggtgaccgtg tcgcccatga
     1681 acaccgcgcc gaatgcgccc acatcgttcg caacgggttt cgccttgacc tgcttgtctc
     1741 cgtacagcca gtcttcaaac cgcttgttgg cagtcggccc ttctgggatc agggcgacca
     1801 cgcgcccgcc gtccgtgagg tgccggaaag ccttgtcgag gtgatcgatc gccgtctttc
     1861 cgccggtgcc gaacggcgga ttcatcacga tcgcgtcgta cttgttgatc gtgtcgtgac
     1921 cctcgaagcg ctcgttgatg agcttggcgt cggtcgccag cgtcaggcgc gaagcgagtt
     1981 caagcgacgg ctcgatgacc gtccgatcgc tcttttccgg gaaccagcgc gcgatcgcgc
     2041 cgtggcccgc agatggctcc aggacgctct cacccgtcag gatgccggcc cactcgacca
     2101 tcttgagccc cagcggctcg ggcgtggcgt agtagtccac gccctcggcg gccttggtcc
     2161 tggacgtccg cttctgctgc gcgaagtaga gcgatcgggc gcggtcccac tcggtgatcg
     2221 cgccgaccag atcgcgatcg ccggccttcc cgcccctgcc gtccgctgcg cctggctcgt
     2281 aatccgggtc gctgttctcg aacgcctgga tgaacgcctc gcgaagcccg cgcgcctgct
     2341 cgcccatcgc caggttctcc gccgtgccgg cgcggcgggc tatcttcgat ccgaaggcgt
     2401 agcgctccca gttcgtgccc gtgttgaagt actggaggtt ggcgtccgac ttctggccca
     2461 cccggtaaat gcgcccctct tcctggatcg cctcgatcgg cgccaccggc aaacccaggt
     2521 tgatcagaac ccgaccgtgc ttgcctgtcg tgtcgtgacc agaccatccg gcgttggcgg
     2581 ccttctgcac cagcaacagg ttcgcttctg gcttggcatc gtcgttgaag tctcgaatcg
     2641 actggacgcg cgtcttcttg tactgactca tgccgttgta gacgccggcg cccgggaagg
     2701 cctcgagcag cgtcgtcagc ggcgacccat actggctgaa gtcgatcgct tgcagctccg
     2761 gacgggcggc catgaactcc cggatcagtt gcgagcgcgg aacattctcg cgcttgatct
     2821 ggccgtgcac gaccttcccc tgcccacgaa caggaacgtc gaccccatcc ttgccgacag
     2881 agtaatcgga cgccttctcg gcggcctctg cccgcttcag ttgctcgctg aggttgaaga
     2941 cgttgatccc gccgccactg ttgaagtcgt agaacacgac gaccttgcgc ccaagggcat
     3001 ggtgcttctt gatgtacggg atggctgcgc gcgccttgat ggcctccagc aggcgcgagc
     3061 gcgtcaggta gtcgaactgc tcgttgatgg cgccatagag gctccagtac cgcccgttgt
     3121 ccgtctcgcg cagccaccgc aggccgtcgt cgatggtctg gccgacgccg ccctggatga
     3181 ggatgaattt ccgctcgtag tcgaagtcga cgtcgagcat gcgcgcggac agtaccccct
     3241 ccttgcgcag ccaggtgttg aactgacgct gcatcaggtc gctgtccact tcggccggcg
     3301 gcttgttcag cttgccgttg cgcatgcgcc agccgaaatg ctgcatcatg aacttctggt
     3361 atccgttcgg cgcgttgtag ccgcgcccgc tgaccttcgg gaactcgtac aggtaccctt
     3421 cgccccactg gatgcttggc tcgtaggcaa acggggtcgc cgacaggaag attgcgcgag
     3481 tgcgcttctc gccctgtcga gccatgacgt cgctctcgac ggcatcgagg accgccttcc
     3541 acttggcccg gtctgccgcc agttgtttct cgagcccgtc tgcctcggcc cggtacgccg
     3601 cgtacatctg gtccatgatg tccgggttgt tcatcatgcg gtagagggac ttcagttggt
     3661 ccgacgtggt gctgatgcgg tcgaccagtt cccggttctg catctcggcg cgctgcagca
     3721 cgccgtctgg gtgaagactc accgcacgca gagcgtccag cgccttcgtt ggctcggcgt
     3781 ccttgccgag catgaggagg tgcgcttcgt cgatcgccac aaagtcgtag tcgcgcttgg
     3841 cgatctcgtc gttctgcccg aagttggcgt aggtcgtgat ggcgatcccg gacccggcat
     3901 cctgggtgtc ggacagcggc ttgatctgca gaccgagcgc cggcgcgctc ttcacccact
     3961 cgttcatcac cgcctcgttc ggcacgacga tgatgccgtt gcccttgccc cgcttggtga
     4021 ggcgcttgat cgctccgagt gtcaggaagg tcttgccggt ccccgtgccg ttggtgaac
//
LOCUS       DAOQNN010000292         4075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14105_length_4075_cov_9.098010, whole genome shotgun sequence.
ACCESSION   DAOQNN010000292 DAOQNN010000000
VERSION     DAOQNN010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14105_length_4075_cov_9.098010"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(359..1105)
                     /gene="ydfG"
                     /locus_tag="PKV97_13170"
     CDS             complement(359..1105)
                     /gene="ydfG"
                     /locus_tag="PKV97_13170"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HNB06876.1"
                     /translation="MIVFVTGASAGFGAAIARSFVKGGHRVVATARRKDRLDALAAEL
                     GDALLALELDVRDADAVAAVPGVLPDGFAEVDVLVNNAGLALGLEPAHKASLEDWHTM
                     IDTNCTGLVQVTRAFLPGMVARNRGHIFNLGSVAGRWPYAGGNVYGATKAFVRQFSLN
                     LRADLLGTALRVTDIEPGLCGGTEFSNVRFHGDDDKAAKVYADVQPLTAEDIADSVYW
                     IATRPAHVNINTIELMPVAQSFAGLSVHRG"
     gene            1369..2376
                     /locus_tag="PKV97_13175"
     CDS             1369..2376
                     /locus_tag="PKV97_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB06877.1"
                     /translation="MKLGPLRSTLLTLALATGVAGVSHAQTTLLNVSYDPTRELYQDF
                     NPEFAKHWKARTGETVTIKQSHGGAGKQARAVIDGLEADVVTLALAYDIDAIAEQTGK
                     IPADWQKRLANNSSPYTSTIVFLVRKGNPKGIKDWGDLVKPGVEVVTPNPKTSGGARW
                     NYLAAWAYALKQPGGNEQTAQAFVTELIKHVPVLDSGARGATNTFVQRGIGDVLLAWE
                     NEAFLSINELGPDKFEIVVPSISIRAEPPVTVVDGVAKKRGTEKLAQAYLEYLYSPVG
                     QKLAAKHYYRPVRPEHADPADVARFPKVELITIEDLGGWQAAQKKHFADGGVFDQIYA
                     K"
     gene            2392..2607
                     /locus_tag="PKV97_13180"
     CDS             2392..2607
                     /locus_tag="PKV97_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNB06878.1"
                     /translation="MAIQAINVRNQFRGRVKEIIRGEVVSEVDVETSSGIVTSVITTR
                     SVDELGLKPGSEVVALVKSTEVSIATL"
     gene            2615..3307
                     /locus_tag="PKV97_13185"
     CDS             2615..3307
                     /locus_tag="PKV97_13185"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB06879.1"
                     /translation="MRQTVLIVPGYRGSGPGHWQSWLEGELPEARRVGGIDWDTPLLA
                     RWAGEVRREIDASAGAVWIVAHSFGCLASVVAAVDRPQKVAGLLLVAPADPERFELLG
                     VREDPQRSAGVGRFLPQSGLPCPSFMVVSRSDPWLSQDVAHEWAERWGSRLIDAGDAG
                     HINVESGHGPWPLAPSLLQQLRDSAGDLPLGMITPDQRAPLRGRSSALARLRHQTRRF
                     LSLRRTTMPSTP"
     gene            3345..>4075
                     /locus_tag="PKV97_13190"
     CDS             3345..>4075
                     /locus_tag="PKV97_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB06880.1"
                     /translation="MIRCQEFRGLAPAQECNMPESNTAKLLTLPNAGQGALSIRAKAL
                     IFHDAKSLRLLRQVELIAPTEANVLIIGETGTGKELVARHIHAQSGRSGPFVAVNCGA
                     FSDNLVEAELFGHEAGAFTGAQQARAGWFEAANGGTLFLDEVGDLPLAMQVKLLRVLQ
                     ERQVVRLGSRKPIPVDVRLVAATNVNLEKAVQAGHFRLDLYYRLNVAPVELPPLRERP
                     GDILPLVEYFIDFYRQRLDIPTPRL"
BASE COUNT          690 a         1431 c         1382 g          572 t
ORIGIN      
        1 cccgccacgc tcgccaccag cagcgccacg cagatggcaa cgcccagcac gcgccagggg
       61 cgcgggcggc gcccgacgga aggcactgcg ggctccggag cggaaggcgg aagatctgga
      121 gtcacggtcg ggcggatcga tggtgcattg cggaaattat gcgcatcctg ctgcaagaaa
      181 atgacactgt tacaaactcg ccacaggcac ggagtcgtcc gcaggccgcc cgcctcgccc
      241 ccccgggcag gcgcatgcgg ccatcgtcgc aagcgaaccc gacgtggcgc cagcgcgagg
      301 gagcggcgac ggcgccctgc ccgctccgct gcggatgtgg tcgcacgccg gccagggctc
      361 agccgcgatg caccgacaag ccggcgaagg actgcgccac cggcatcagc tcgatggtgt
      421 tgatattgac gtgcgccggc cgggtggcga tccagtacac cgagtcggcg atgtcctcgg
      481 cggtcagcgg ctgcacgtcg gcatacacct tggccgcctt gtcgtcgtcg ccgtggaagc
      541 gcacgttgga gaactcggta ccgccgcaca gcccgggctc gatgtcggtg acgcgcagcg
      601 cggtgccgag caggtcggcg cgcaggttga ggctgaactg gcgcacgaag gccttggtgg
      661 cgccatagac gttgccgcca gcatagggcc agcgtccggc caccgagccg aggttgaaga
      721 tgtggccgcg gttgcgcgcc accatgccgg gcaggaaggc gcgggtgacc tgcaccaggc
      781 cggtgcagtt ggtgtcgatc atggtgtgcc agtcttccag cgaggctttg tgcgcaggtt
      841 cgaggccgag cgcgaggccg gcgttgttga ccagcacgtc cacctcggcg aagccgtcgg
      901 gcagcacgcc cggcaccgcg gcgaccgcat cggcgtcgcg cacgtcgagc tcgagcgcca
      961 gcagcgcgtc gccgagttcg gcggcgagcg catccaggcg gtccttgcgg cgtgcggtgg
     1021 cgacgacgcg gtggccgccc ttgacgaagc tgcgcgcgat ggcggcgccg aagccggcgg
     1081 aggcgccggt gacgaagacg atcatttgtg gaattcccca tgcgaaggcg tgacaggccg
     1141 cgccgcggtc gcggggcggg cgaaagaacg cgagatgtta gcgaacccgg tcgtggattc
     1201 caaagacggg ccgcgcgcgc agagggctcg tcgtgcgcgg ggtcgtcgct ctcggggtcg
     1261 tcgccccccg agccgccgag cgaataagca atccgttttt tcttggttcg ggtgaaggcg
     1321 cacgaaggct aaattttcgc catcgcactc acccatccgg agaaagaaat gaagctcggc
     1381 ccccttcgca gcaccctgct caccctggcg ctcgccaccg gcgtcgccgg cgtctcccac
     1441 gcgcagacca ccctgctcaa cgtctcctac gaccccacac gcgagctcta ccaggacttc
     1501 aaccccgagt tcgccaagca ctggaaagcc cgcaccggcg agaccgtcac catcaagcag
     1561 tcccacggcg gcgccgggaa gcaggcgcgc gcggtgatcg acggcctcga agccgacgtg
     1621 gtcacgctgg ccctggccta cgacatcgac gccatcgccg agcagacggg caagatcccc
     1681 gccgactggc agaagcgcct ggccaacaac agctcgccct acacctcgac catcgtcttc
     1741 ctggtacgca agggcaaccc caagggcatc aaggactggg gcgacctggt caagccgggc
     1801 gtcgaggtcg tcacccccaa ccccaagacg tccggcggcg cgcgctggaa ctacctcgcc
     1861 gcctgggcct acgcgctcaa gcagccgggc ggcaacgaac agaccgcgca ggccttcgtc
     1921 accgaactga tcaagcacgt gccggtgctg gattcgggcg cccgcggcgc caccaacacc
     1981 ttcgtccagc gcggcatcgg cgacgtgctg ctggcgtggg agaacgaggc cttcctgtcg
     2041 atcaacgaac tcggcccgga caagttcgag atcgtcgtgc cctcgatctc gatccgcgcc
     2101 gaaccgccgg tcaccgtcgt cgatggcgtg gcgaaaaaac gcggcaccga gaagctcgcc
     2161 caggcctacc tcgagtacct gtactcgccg gtcgggcaga aactcgccgc caagcactac
     2221 taccgcccgg tcaggcccga acacgccgac ccggccgacg tcgcccgctt cccgaaagtc
     2281 gagctgatca ccatcgagga cctcggcggc tggcaggcgg cgcagaagaa gcactttgcc
     2341 gacggcgggg tgttcgacca gatctacgcc aagtaagcga ggagcactgc gatggcaatc
     2401 caggcaatca acgtccgcaa ccagtttcgc ggccgggtga aggaaatcat ccgtggcgag
     2461 gtggtcagcg aagtcgatgt ggaaacttcc tcgggcatcg tcacctcggt gatcaccacg
     2521 cgctcggtcg acgagctcgg gctcaagccc ggctccgagg tcgtcgcgct ggtgaagtcc
     2581 accgaagtgt cgatcgccac gctgtgaggc cgtcatgcgc cagacggtgc tgatcgtccc
     2641 cggctaccgc ggcagcggcc ccgggcactg gcagagctgg ctcgagggcg aactgcccga
     2701 ggcccggcgc gtcggcggca tcgactggga caccccgctg ctcgcgcgct gggcaggcga
     2761 ggtacggcgc gagatcgacg cctcggccgg tgcggtgtgg atcgttgcgc acagcttcgg
     2821 ctgcctcgcc agcgtggtgg ccgcggtgga tcggccacag aaggttgcag gcctgctgct
     2881 ggtggcgccc gccgatccgg agcgcttcga actcctcggc gtgcgcgagg acccacagcg
     2941 cagcgccggg gtcggccgct tcctgccgca atccggcctc ccctgtccca gcttcatggt
     3001 cgtcagccgc agcgacccgt ggctgagcca ggacgtggcg catgaatggg ccgagcgctg
     3061 gggaagcagg ctgatcgatg cgggcgatgc aggacacatc aatgtcgagt ccggtcacgg
     3121 gccctggccg ctcgctccga gcctgttgca gcaactccgc gacagcgcgg gcgacctccc
     3181 gctcgggatg atcacgccgg accagcgagc gccgttgcgc ggccgcagca gcgccttggc
     3241 ccggctgcgg caccagacgc gacgcttcct gagcctgcgc agaacgacca tgccgagcac
     3301 gccctgaccg ctgcgctttc cccactgcaa cacgagcgcc gctcttgatc cgctgccagg
     3361 agttccgcgg cctcgccccc gcacaggagt gcaacatgcc cgaatcgaac accgccaagc
     3421 tgctgacctt gcccaacgcc gggcaaggcg cgctctccat ccgcgccaag gcgttgatct
     3481 tccatgacgc caaatcgctg cgcctgctgc gccaggtcga gctcatcgcg ccgaccgaag
     3541 ccaacgtgct gatcatcggc gagaccggca ccggcaagga gctggtggcg cgccacattc
     3601 acgcgcagag cggacgcagc ggccccttcg tggcggtcaa ttgcggcgcg ttcagcgaca
     3661 acctggtcga ggccgagctg ttcggccacg aggccggcgc cttcaccggc gcccagcagg
     3721 cgcgcgccgg ctggttcgag gcggccaacg gcggcacgct gttcctcgac gaggtcggcg
     3781 acctgccgct ggcgatgcag gtcaagctgc tgcgcgtgct gcaggagcgc caggtggtgc
     3841 ggctgggctc gcgcaagccg atcccggtgg atgtacgcct ggtggcggcg accaacgtca
     3901 atctggagaa ggcggtgcag gccgggcatt tccgcctcga cctctactac cggctcaacg
     3961 tcgcgccggt ggagctgcca ccgctgcgcg agcgcccggg cgacatcctg ccgctggtcg
     4021 agtacttcat cgacttctac cgccagcgcc tcgacatccc gacaccgcgc ctgag
//
LOCUS       DAOQNN010000293         4065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14159_length_4065_cov_8.950125, whole genome shotgun sequence.
ACCESSION   DAOQNN010000293 DAOQNN010000000
VERSION     DAOQNN010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14159_length_4065_cov_8.950125"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1952)
                     /locus_tag="PKV97_13195"
     CDS             complement(105..1952)
                     /locus_tag="PKV97_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNB06881.1"
                     /translation="MSAPARYTLGIDLGTTHCALSYVDLEASDGDQAAQQVLEIPQLT
                     GPGSIEARPLLPSFLYLPHADELAPGDLSLPWGATQDHAVGEFARSRGAATPIRLVAS
                     AKSWLCHPGVDRRADILPAEAPPEVARISPLTASVRYLSHLRAAWNATHPEAPFDAQD
                     VTVTIPASFDPAARELTAEAAQAAGYPPITLLEEPQAALYSWIQRSAGQWRKEVKTGD
                     LILVVDVGGGTTDLSLIAVLEREGNLELHRVAVGEHILLGGDNMDLALAYTVTRKLAE
                     QGTRLDPWQTRALAHACRGAKEALLSDSDLDSVPVVVPSRGSKLIGGSIRTEVRREEL
                     VATLVEGFFPRVAVTEQPITRARAALTQLGLPYAQDAAVTRHLAAFLTRQLGATADLE
                     GFAAQPEESVFLHPTAVLFNGGVLKSGLIAGRVLDTLNDWLAADGAAPARLLGGADLD
                     LAVARGAAYYGYVRRGRGVRIRGGTARAYYVGIESSMPAIPGMEPPLEALCVAPFGME
                     EGSEAALPAQEFGLVVGEPVRFRFFGSSVRRQDEVGTLLDFWQPDELQELEEIEATLP
                     AEGRRPGEVVRVKLHARVTEAGTLELEALPVDGSARWKVQFDVRGNAEA"
     gene            complement(1949..2503)
                     /locus_tag="PKV97_13200"
     CDS             complement(1949..2503)
                     /locus_tag="PKV97_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HNB06882.1"
                     /translation="MASRNPSFFRRIGLAFSVLFGAELAGRLVRLREEGADTEAAAGT
                     EDAAAAAQPAIVRETTPEAALQLLGLLQRDARFIDFVQEDIAAYSDADIGAAARVVHE
                     GCRKVLDEHFAITPVRDEAEGSRLTLPEGFDAAAVRLTGKVVGKPPFTGTLGHRGWRV
                     TDTRLPALAEGHDASVIAQAEVEL"
     gene            complement(2595..3941)
                     /locus_tag="PKV97_13205"
     CDS             complement(2595..3941)
                     /locus_tag="PKV97_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNB06883.1"
                     /translation="MKSALFVDFDNVYSGLRKLDPAIADRFARQPLEWVNWVIGALEL
                     PDHAPAGARRRLLVRRCYLNPQAYQRFRPSFNLAGFEIIDCPALTSEGKTSTDIHMVL
                     DIIDLLQHEARYDEFIVFSADADFTPVLRKLRRWDRRTTVLAIGFPSAAYRASADLLI
                     DPDEFVRNALGFKDEDEASGAGAPPADVAPESLSLAASARFAQASPEAARSVPPASLS
                     SGADALDHDHAEIVETMRAEVARSAAPVPCSRLASLITTRHPALAADWNGKGSFRRFV
                     DSLDLAPLRFDWSNSGGFVFDPARSGAGMLDGNATPDWGEDQDILPLAMQIHEVSGVP
                     LLGPAEYRALFSIIEDDVGRHPFDLKETGKRVRDRLREAGLGGSRLDVNWILRGLLMR
                     GHAFGEGQDRAATLATKTANNVRSLCLREQMILDRAAETAIARWFGTHPAPTRSGQ"
BASE COUNT          537 a         1315 c         1639 g          574 t
ORIGIN      
        1 ccagcacctg gatgcggtcc tcgccctctg cggcgaaggc gaccacggtg ttgctggtgc
       61 cgaggtcgat gccgacggtg tagcgcgtcg tcacgcgagc gacctcaggc ctcggcgttg
      121 ccgcgcacgt cgaactgcac cttccagcgc gcgctgccgt ccaccggcag ggcctcgagc
      181 tcgagcgtgc cggcctcggt gacgcgggcg tgcagcttca cgcgcacgac ctcgccgggg
      241 cggcggcctt cggcgggcag ggtggcctcg atctcctcga gctcctgcag ctcgtcgggc
      301 tgccagaagt cgagcagggt gccgacctcg tcctggcggc gcaccgagga gccgaagaag
      361 cggaagcgca ccggctcgcc gaccaccagg ccgaactcct gcgcgggcag ggcggcctcg
      421 ctgccctcct ccatgccgaa gggggcgacg cagagggctt cgagcggcgg ctccatgccc
      481 gggatggcgg gcatcgagga ctcgatgccg acgtagtagg cgcgcgcggt gccgccgcgg
      541 atgcgcaccc cgcgtccgcg gcgcacgtag ccgtagtagg ccgcgccgcg cgccaccgcg
      601 aggtcgaggt cggcgccgcc gaggagccgc gccggcgcgg cgccgtcggc ggcgagccag
      661 tcgttcaggg tgtcgagcac gcgcccggcg atcagcccgg acttgagcac gccgccgttg
      721 aacagcaccg cggtggggtg caggaagacg ctctcctcgg gctgcgcggc gaagccttcg
      781 agatcggcgg tggcgccgag ctggcgggtg aggaaggcgg cgaggtggcg ggtgaccgcc
      841 gcgtcctgcg catagggcag gccgagctgg gtgagcgcgg cgcgcgcccg cgtgatgggt
      901 tgctcggtga ccgcgacgcg ggggaagaag ccctcgacca gggtggcgac cagctcctcg
      961 cgccgcacct cggtgcggat cgagccgccg atcagcttcg agccgcgact cggcaccacc
     1021 accggcacgc tgtcgaggtc ggagtcgctc agcagcgcct ccttggcgcc gcggcaggca
     1081 tgggcgagcg cgcgcgtctg ccaggggtcg agccgggtgc cctgctcggc gagcttgcgc
     1141 gtgaccgtgt aggccagcgc caggtccatg ttgtcgccgc cgagcaggat gtgctcgccc
     1201 accgccacgc ggtgcagctc gaggttgccc tcgcgctcga gcaccgcgat cagcgacagg
     1261 tcggtggtgc cgccgccgac gtcgaccacc aggatcaggt cgccggtctt cacttccttg
     1321 cgccactgcc cggcgctgcg ctggatccag ctgtagagcg ccgcctgcgg ctcctcgagc
     1381 agggtgatcg gcgggtagcc ggcggcctgc gcggcctcgg cggtgagctc gcgcgccgcg
     1441 gggtcgaagg aggccgggat ggtgacggtg acgtcctggg cgtcgaaggg cgcctcggga
     1501 tgggtggcgt tccaggccgc gcgcaggtgc gacaggtagc gcaccgaggc ggtcagcggc
     1561 gagatgcgcg ccacctcggg cggcgcctcg gcgggcagga tgtcggcgcg gcggtcgacg
     1621 ccggggtggc acagccagct cttggcgctg gccaccaggc ggatcggcgt ggccgccccg
     1681 cggctgcggg cgaactcgcc caccgcgtga tcctgcgtcg ccccccaggg caggctcagg
     1741 tcgccggggg cgagctcgtc ggcgtgcggc agatagagga aggagggcag cagcgggcgc
     1801 gcctcgatgc tgcccgggcc ggtgagctgg ggaatctcca gcacctgctg cgcggcctgg
     1861 tcgccgtcgc tggcttcgag gtcgacatag gacagcgcgc agtgggtggt gccgaggtcg
     1921 atgccgaggg tgtagcgggc gggggcgctc acagctccac ctccgcctgg gcgatcacgc
     1981 tggcgtcgtg gccttcggcc agcgccggca ggcgggtgtc ggtcacgcgc cagccgcgat
     2041 ggccgagggt gccggtgaag ggcggcttgc ccaccacctt gccggtgagg cgcacggccg
     2101 cggcgtcgaa gccttcgggc agggtgaggc ggctgccttc ggcctcgtcg cgcaccggcg
     2161 tgatggcgaa gtgctcgtcg agcaccttgc ggcagccctc gtgcactacg cgcgcggcgg
     2221 cgccgatgtc ggcatccgaa taggcggcga tgtcttcctg gacgaaatcg atgaagcgtg
     2281 cgtcgcgctg gagcaggccg agcagctgca gcgcggcctc gggggtggtc tcgcgcacga
     2341 tggcgggctg ggcggctgcg gcggcgtcct cggtgccggc agcggcctcg gtgtctgcgc
     2401 cttcctcgcg caggcggacg aggcggccgg cgagctcggc gccgaagagg acggagaagg
     2461 cgaggccgat gcggcggaag aaggaggggt tgcgactggc catgggggtg gtcctcgtgc
     2521 tggatctgtt gcgacgcaat atagctcaga agcgtgtggg aggcggagtc gagggccgcg
     2581 gcacccgcat cggctcactg cccgctgcgg gtgggagccg gatgggtgcc gaaccagcgt
     2641 gcgatcgcgg tctcggccgc gcggtcgagg atcatctgct cgcgcaggca gagcgagcgc
     2701 acgttgttcg ccgtcttggt ggcgagcgtc gcggcgcggt cctgcccctc gccgaaggcg
     2761 tggccgcgca tcagcagtcc gcgcaggatc cagttgacgt cgagccggct gccgccgaga
     2821 ccggcttcgc gcaggcggtc gcgcacgcgc ttgccggttt ccttgaggtc gaagggatgg
     2881 cggccgacgt cgtcctcgat gatcgagaac agcgcgcggt attcggccgg gccgagcagg
     2941 gggacgccgg agacctcgtg gatctgcatg gcgagcggca ggatgtcctg gtcctcgccc
     3001 cagtcgggcg tggcgttgcc gtccagcatg ccggcgccgg agcgggcggg atcgaagacg
     3061 aagccgccgc tgttgctcca gtcgaaacgc agcggcgcga ggtcgaggct gtcgacgaac
     3121 ctgcggaagc tgcccttgcc gttccagtcg gcggcgaggg cggggtggcg cgtggtgatc
     3181 aggctcgcca ggcggctgca ggggacgggc gcggccgagc gcgcaacctc ggcgcgcatg
     3241 gtctcgacga tctcggcgtg gtcgtggtcg agggcgtcgg cgccgctgga gagcgaggcc
     3301 gggggaacgc tccgggccgc ttccggcgac gcctgcgcga aacgtgcgct cgctgcgagc
     3361 gacaggcttt ccggggcgac gtcggcaggg ggcgcgccgg cgccggacgc ctcgtcctcg
     3421 tccttgaagc cgagggcgtt gcggacgaac tcgtcggggt cgatcagcag gtcggccgag
     3481 gcgcgatagg ccgcggacgg aaagccgatc gccagcaccg tggtgcgccg atcccagcgc
     3541 cgcagcttgc gcagcaccgg ggtgaagtcg gcgtcggccg agaacacgat gaactcgtcg
     3601 tagcgggcct cgtgctgcag caggtcgatg atgtcgagca ccatgtggat gtcggtgctg
     3661 gtcttgccct cgctcgtcag cgccgggcag tcgatgatct cgaagccggc gaggttgaag
     3721 gacgggcgga agcgctggta ggcctgcggg ttgaggtagc agcgccgcac cagcagccgg
     3781 cggcgcgcgc cggcgggggc gtggtcgggc agctcgagcg cgccgatcac ccagttcacc
     3841 cactccaggg gctggcgggc gaagcggtcg gcgatggccg ggtcgagctt gcgcaggccg
     3901 gaatagacgt tgtcgaagtc gacgaagagg gcgcttttca agatgtctcg cggggatggg
     3961 gggtcaggta gggctcgtgg ccgggccgtg ggggtgggcg tcgtgtggtg gcatttctgg
     4021 aagtgtgatt cagttgaaac aagcacttgc gtgctgaact gtcga
//
LOCUS       DAOQNN010000294         4041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14315_length_4041_cov_9.150778, whole genome shotgun sequence.
ACCESSION   DAOQNN010000294 DAOQNN010000000
VERSION     DAOQNN010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14315_length_4041_cov_9.150778"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..237)
                     /locus_tag="PKV97_13210"
     CDS             complement(4..237)
                     /locus_tag="PKV97_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06884.1"
                     /translation="MAQPTLKQRKTFALIRIFGGMVAALYLSFVVVTNMLAGHALEGE
                     LLYSALVALAGYGYAAWYLRELAAVAREERGGR"
     gene            414..1841
                     /locus_tag="PKV97_13215"
     CDS             414..1841
                     /locus_tag="PKV97_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNB06885.1"
                     /translation="MHAPVSGSPGTAPAAPSPALSLSPALLFLLVFVEGFVSLGAEIL
                     ALRRLVPHVGSAITVTAPTIAFFLLALALGYQAGGQVAGDYLARVRRNFLLAAALIAL
                     GLAGPVVELAFAHLQPAPVAWAVVVCGVLCPVAWLLGQTVPILTNLLRHERVGARSGA
                     ALYWSTLGSFLGALTLSMGVMQWLGVGAAVLVCAGLLLTAVPALGRAAGARLPSAAAA
                     LAIAGMALVANLVLPQQIETAYATYRVEAVAIPGYVAPEVLRINNSPASILDADEPPR
                     YARYIRRLRDLLLDELGFRGRSVLVLGAGGFTLSHREPLNRYVYVDIDPAVKEIAERD
                     FLREPVRGEFVAADARAHLRATAARHDAVVVDVYSALTSIPAHLVTREFWRATRAVLK
                     PGGVLAANLILDRELASPYARSLLASIQAEYGACAVEVLFRDRPQGNVLVICSAPSTA
                     HAAQEPALPYTDERNRADLDVLRGS"
     gene            complement(1842..4040)
                     /locus_tag="PKV97_13220"
     CDS             complement(1842..4040)
                     /locus_tag="PKV97_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB06886.1"
                     /translation="MSFPDAPVPRCCMNIKQPVVDAGGRPVGVIGLAIDIEARKQQER
                     ELVGLKNDYAAILQALPDPLFEFDIEGRCHGYRTPDEDLLAAPPEVFLGRTVREVLPP
                     EEAEVCLEALREAAAKGRSTGKVFKVEAPQGTRWFELSVAPMASAAGRTQRFNVLSRE
                     VTARERMERALQEQESLLRAVVDNTPVEYWARDLDGRCTLQNAMTVAHWGDLLGQRPE
                     ESAKEPAARTEWLETNRRAYAGETVHREVEYEVDGETRIFQCLVAPIRVGGEVVGIFG
                     FNQDITERKRQEHQIHELAFFDPLTRLPNRRLMFDRLERALGSSARRRHQGALLLIDL
                     DHFKELNDSHGHEAGDQLLMKVAARLGLSIREGDTAARLGGDEFVVILEDLEDVADEL
                     LHIKAVADRIGRELGRPFQLQRGTAGETISYQCSASIGISAFGEPGVGAAELLRRADT
                     ALYQAKAAGRNAVSFFDPIMQAAVTARARLHSDLRQAIRGGQFELHYQVQVDAQRRPA
                     GAEALVRWRHPEKGLIHPGGFIDLAEETGLIVPLGHWVLATGCRQLALWARHPATAHL
                     RLAVNVSARQFRQPSFADEVRALLEQTGAPADRLKLELTESSLIEDTEAVIERMETLR
                     ALGIRFALDDFGTGYSSLAYLKRLPLAQLKIDQSFVRDLMSDPNDASIVRTILALGDS
                     LGLAVIAEGVETEAQYAFLAAHGCRAYQGYLFGRPVALDVFEAGLAASDG"
BASE COUNT          539 a         1480 c         1423 g          599 t
ORIGIN      
        1 ccctcatcgg ccgccgcgct cctcgcgcgc caccgcggcg agctcgcgca ggtaccaggc
       61 cgcgtagccg tagccggcga gcgcgaccag ggcggaataa agcagctcgc cttcgagcgc
      121 atggccggcg agcatgttgg tgacgacgac gaagctcagg tagagcgcgg cgaccatgcc
      181 gccgaagatg cggatgaggg cgaaggtctt gcgctgcttg agggtgggct gggccatggg
      241 tagacggggc gggacaagga gacaggcggc agggtagccg attccggcct tgccggcatc
      301 ggccggggga gcgcagcgca acactgcgca caggcgtgct ggcctccggc ggcaacgcgc
      361 cggataatcg gtcgcctcca aggcgccccc ttcaccacgc gaggacctcc gcgatgcacg
      421 ctccggtctc cggcagcccc gggacggcgc ccgccgcgcc ctcccccgcc ctgtcgctgt
      481 cgcccgccct gctcttcctg ctcgtcttcg tcgagggctt cgtctcgctc ggcgccgaga
      541 tcctcgcgct gcgccggctg gtgccgcacg tgggcagcgc gatcaccgtc accgcgccga
      601 cgatcgcctt cttcctgctc gcgctggcgc tcggctacca ggccggcggg caggtggccg
      661 gcgactacct cgctcgcgtg cggcgcaact tcctgctcgc cgcggcgctg atcgcgctcg
      721 ggctggcggg tccggtggtg gagctggcct tcgcccacct gcagccggca ccggtggcct
      781 gggcggtggt ggtgtgcggc gtgctgtgcc cggtggcctg gctgctcggc cagaccgtgc
      841 ccatcctcac caacctgctg cgccacgagc gcgtcggcgc gcgcagcggg gcggcgctgt
      901 actggtccac gctgggctcc ttcctgggcg cgctgacgct gtcgatgggg gtgatgcagt
      961 ggctgggcgt gggcgcggcg gtgctggtgt gcgccggcct gttgctcaca gcggtaccgg
     1021 cgctcggccg cgcggctggt gcgcgcctgc cgtcggccgc ggcggcgctc gcgatcgcgg
     1081 gcatggcgct ggtggccaac ctggtgctgc cgcagcagat cgagaccgcc tacgccacct
     1141 accgcgtcga ggcggtggca atccccggct acgtggcacc cgaggtcctg cgcatcaaca
     1201 attcgccggc ctccatcctc gacgccgacg agccgcctcg ctatgcgcgc tacatccgcc
     1261 gcctgcgcga cctgctgctc gacgagctcg gcttccgcgg acgcagcgtg ctggtgctgg
     1321 gcgcgggggg cttcacgctc tcgcaccgcg agccgctcaa tcgttacgtc tatgtcgata
     1381 tcgaccccgc ggtgaaggag atcgccgagc gcgacttcct gcgcgagccg gtgcgcggcg
     1441 agttcgtcgc cgccgacgcg cgcgcccacc tgcgcgcgac cgccgcgcgc cacgacgcgg
     1501 tggtggtcga cgtctacagc gcgctcacca gcatccccgc ccacctggtc acgcgcgagt
     1561 tctggcgcgc cacccgtgcc gtgctgaagc cgggcggcgt cctcgccgcc aacctgatcc
     1621 tcgaccgcga gctcgccagc ccctacgcgc gcagcctgct ggcgagcatc caggccgagt
     1681 acggcgcgtg cgcggtcgag gtgctgttcc gcgaccgccc gcagggcaac gtgctggtga
     1741 tctgctccgc cccgtccacg gcgcacgcgg cgcaggaacc cgccctgccc tacaccgacg
     1801 agcgcaaccg cgccgacctc gacgtgctgc gcggcagctg atcagccgtc cgacgccgcc
     1861 agcccggcct cgaacacgtc cagcgcgacc ggccgcccga acagatagcc ctggtaggcg
     1921 cggcagccgt gcgccgccag gaaggcatac tgcgcctcgg tctcgacccc ctcggcgatc
     1981 acggcgaggc cgaggctgtc gccgagggcg aggatggtgc gcacgatgct ggcatcgttg
     2041 ggatcggaca tcaggtcgcg cacgaaagac tggtcgatct tcaactgcgc cagcggcagg
     2101 cgcttgagat aggcgagcga ggagtaaccg gtgccgaagt cgtcgagcgc gaaacggatg
     2161 ccgagcgcac gcagcgtctc catgcgctcg atcaccgcct cggtgtcctc gatcaggctg
     2221 ctctcggtca gctcgagctt gaggcggtcc gccggcgcgc cggtctgttc gagcagcgca
     2281 cgcacctcgt cagcgaagga cggctgacgg aactgacgcg cgctcacgtt aaccgcaagc
     2341 cgcaggtgcg cggtcgcggg gtggcgggcc cacagcgcga gctgccggca ccccgtcgcc
     2401 agcacccagt ggccgagcgg cacgatcagc ccggtttcct cggcgaggtc gatgaatccg
     2461 cccgggtgga tcagcccctt ctcgggatga cgccagcgca ccagcgcctc ggcgcccgcg
     2521 ggccgtcgct gcgcatccac ctggacctgg taatgcagct cgaactggcc tccgcggatc
     2581 gcctggcgca ggtcggaatg caggcgcgcg cgcgcggtga ccgcggcctg catgatcggg
     2641 tcgaagaagc tgaccgcgtt gcgccccgcg gccttggcct ggtagagcgc ggtatcggca
     2701 cgacgcagca gctcggccgc gccgacgccc ggttcgccga aggcgctgat gccgatgctg
     2761 gccgagcact ggtaggagat cgtctcgccc gccgtgccgc gctgcagctg gaagggtcgg
     2821 ccgagctccc ggccgatgcg gtccgcgacc gccttgatgt gcaggagctc gtcggcgacg
     2881 tcctcgagat cctccaggat caccacgaac tcgtcgcccc ccaggcgcgc ggccgtgtcg
     2941 ccctcgcgga tgctcagccc gaggcgcgcg gcgaccttca tcagcagctg gtctccggcc
     3001 tcgtggccgt gggaatcgtt gagctccttg aagtggtcga ggtcgatcag cagcagcgcg
     3061 ccctggtgcc ggcgacgcgc actgctgccc aaggcccgct ccaggcggtc gaacatcaga
     3121 cgccggttgg gcagccgggt cagcggatcg aagaaggcga gctcgtggat ctggtgctcc
     3181 tggcgcttgc gctcggtgat gtcctggttg aaaccgaaga tgcccaccac ctcgccgccc
     3241 acgcggatcg gcgcgaccag gcactggaag atgcgcgtct cgccgtcgac ctcgtactcg
     3301 acctcgcgat ggacggtctc gccggcgtag gcacggcggt tggtctccag ccactcggtc
     3361 cgtgccgcgg gttccttcgc gctctcttcc ggacgctggc cgagcaggtc gccccagtgc
     3421 gccaccgtca tcgcgttctg cagggtgcag cgcccgtcga ggtcgcgcgc ccagtattcc
     3481 accggggtgt tgtccaccac ggcgcgcagc agcgactcct gctcctgcag cgcgcgctcc
     3541 atgcgctcgc gggcggtcac ctcgcgcgaa agcacgttga agcgctgcgt ccggcccgcc
     3601 gcgctggcca tcggcgccac cgagagctcg aaccagcgcg tgccctgggg cgcctccacc
     3661 ttgaacacct tgcccgtgga gcgccccttc gccgccgcct cgcgcagggc ctcgagacag
     3721 acctcggcct cttccggcgg cagcacctcg cgcacggtgc gccccaggaa cacttcgggc
     3781 ggcgcggcga gcagatcctc gtcgggagtg cggtagccgt ggcagcggcc ttcgatgtcg
     3841 aactcgaaca agggatcggg cagggcctgg aggatcgcgg catagtcgtt cttgaggccg
     3901 accagctccc gctcctgctg cttgcgcgcc tcgatgtcga tggcaaggcc gatcacgccg
     3961 accgggcggc cgccggcgtc gacgaccggc tgcttgatgt tcatgcagca gcgcggcacg
     4021 ggcgcgtcgg gaaagctcag a
//
LOCUS       DAOQNN010000295         4014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14472_length_4014_cov_9.415762, whole genome shotgun sequence.
ACCESSION   DAOQNN010000295 DAOQNN010000000
VERSION     DAOQNN010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14472_length_4014_cov_9.415762"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PKV97_13225"
     CDS             <1..426
                     /locus_tag="PKV97_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06887.1"
                     /translation="AAEWTQLARRSEAPEVLARADALLALLAAPALAEAAGANVQALL
                     LRKASDIRSDHDLRVALSQTHVNPLKWLGMAFLGFLTLVSVAMAHLERPRAAFAAVLL
                     FALAAAPTAAIVLIQGNPFQQPSSVTPAPIVAVAKALER"
     gene            787..1494
                     /locus_tag="PKV97_13230"
     CDS             787..1494
                     /locus_tag="PKV97_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB06888.1"
                     /translation="MSASLPRLLTTLAAIGGLAAAGTAHASLINGGFETGTGTPFVAD
                     ANWALFNESAVPGWETTATDNQIEIWGNNFSSVSGGPVPAYEGRQFAEINATQFATLY
                     QDVFGIATGSVVGFEFAHRGRSGVDTMRMMITDLGLDNLLGGGDDTVLFSKDYSTGNT
                     AWAFYTSAGEAPISALGNATRFSYQAISTANGSPSVGNFIDAADFGVGVQAVPEPASI
                     ALIALGLAGLGLRRKRA"
     gene            complement(1556..1918)
                     /locus_tag="PKV97_13235"
     CDS             complement(1556..1918)
                     /locus_tag="PKV97_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB06889.1"
                     /translation="MSAEIQAAGRGNLFAALPPPAGGEIFQTLFANPRCRVERIVSHG
                     HASAPGEWYDQAGDEWVALLAGAAVLRFEGGDDDGDDVKLKAGDWLVIPAHRRHRVES
                     TSADALWLAVHCVADPAK"
     gene            complement(1926..2417)
                     /locus_tag="PKV97_13240"
     CDS             complement(1926..2417)
                     /locus_tag="PKV97_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNB06890.1"
                     /translation="MPKHSTPAELPVQFEPEFIAGIREIFEQKIAFNALLGLQIDDIG
                     GERVHGRIAMRHELVGHFVHNRIHGGVIAACLDAMGGLAVMAAIGARHLDEPPFQRLH
                     RFSKLGTIDLRIDYLRPGIGESFRMEAEVLRLGSRVASTRMSFLDADGKLLSTGSAAY
                     IVS"
     gene            complement(2547..3947)
                     /locus_tag="PKV97_13245"
     CDS             complement(2547..3947)
                     /locus_tag="PKV97_13245"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNB06891.1"
                     /translation="MISVEKIGGTSMSAFGDVLRHIMLYDKSRILGRIYVVSAYSGVT
                     NQLLEHKKTGERGIYALFAEGSDYHGALDALAVSLKKLNAGFAELALPLDVADAFVDT
                     RIGEVKKYLDAMQHVLASGYIRRQDVLLAAREVLASVGESHSAFNSVEILKANGVNAI
                     LLDLAGFHDDKAWTIDERIHNSFKGLDLDNSVIVATGYTKGTEGIMREFDRGYSEVTF
                     SKIAVEVRPAEAVIHKEFHLSSADPNIVGLENAVIVGATNYDVADQLADVGMEAIHPK
                     AAKPMELAGIPIRLKNTFEPEHPGTLITKDYVGERARVEMVTGSDKVTLVEIHDPSMV
                     GTVGFDLGIMEIFCRHDISYILKATNANSIAHVLWENSVTPALIAELEARYQVVTVKN
                     SAIVCAIGSNISIPGVLARAAQALADAQINVNCVSQTLRQVNMQFIIERSDYKKAIIA
                     LNHSLCVTPGVPVPRA"
BASE COUNT          617 a         1381 c         1359 g          657 t
ORIGIN      
        1 gccgccgaat ggacccagct ggcgcgccgc agcgaggcgc cggaggtgct cgcccgcgcc
       61 gacgcgctgc tggcgctgct cgccgccccc gcgctcgccg aggccgcggg cgccaacgtg
      121 caggccctgc tgctgcgcaa ggcgagcgac atccgttccg accacgacct gcgcgtcgca
      181 ctcagccaga cgcacgtcaa cccgctgaag tggctgggaa tggccttcct gggcttcctg
      241 accctggtct cggtggccat ggcccacctc gaacgcccgc gcgcggcctt cgcggcggtg
      301 ctgctgttcg cgctcgccgc ggcccccacc gccgccatcg tgctgatcca gggcaacccc
      361 ttccagcagc cgagctcggt cacgcccgcg ccgatcgtcg cggtcgccaa ggcgctggag
      421 cgctgaagcg ccggtcggcc ctgcggttgc agcgccgtgc cgaggtcccg ccgcgcgccc
      481 tcgcgctgca ggccctgctg cgccttgcga cacccgatcg ggcaaacggc gaatccgtcg
      541 cgatggacgc ggccgccccc ggtcgatggc tgtcggaaaa attgacactg cggcaactcg
      601 agcacgcccg ggatggcccg tgcattcctc tccagaggcc catttcccac tggaatcaac
      661 gcgctgtgac cttctaccgc gcgtgctggc gcgatcattg cttaagatct ggtcgaaact
      721 ctcgggccga cctttcgcgt ctacgcccgt caccgaacgt gtcgcccaaa aataaaggaa
      781 ccacccatgt ccgcctcgct cccccgcctc ctcaccaccc tcgccgcgat cggcggcctg
      841 gccgcggcgg gcaccgccca cgccagcctg atcaacggcg gcttcgaaac cggaacgggg
      901 acgcccttcg tcgccgacgc caactgggcg ctgttcaacg agtcggcggt acccggctgg
      961 gaaaccaccg ccaccgacaa ccagatcgag atctggggca acaacttcag ctcggtgagc
     1021 ggcggcccgg tgcccgccta cgaggggcgc cagttcgccg agatcaacgc cacccagttc
     1081 gccaccctgt accaggacgt gttcggcatc gccacgggca gcgtcgtcgg cttcgagttc
     1141 gcccaccgcg gacgttcggg ggtggacacg atgcgcatga tgatcaccga cctcggcctc
     1201 gacaacctgc tcggcggtgg cgatgacacg gtgttgttct ccaaggacta ctcgaccggc
     1261 aacaccgcct gggccttcta cacgagcgcc ggcgaggcgc cgatcagcgc cctcggcaat
     1321 gccacgcgct tctcgtatca ggccatcagc accgccaatg gcagcccctc ggtcggcaac
     1381 ttcatcgacg ccgccgactt cggcgtcggc gtgcaggcag tgccggaacc ggcgtcgatc
     1441 gcgctgatcg cgctcggcct cgcggggctg gggctgcgcc gcaagcgcgc ctgagacacg
     1501 cgtcgcagtg caggcggttt gacaccacgg ccccggcggt tcgcccgggg ccgtttcact
     1561 ttgcggggtc ggctacgcag tgcaccgcca gccacagcgc atccgccgag gtcgattcga
     1621 cgcggtgacg ccgatgtgcc gggatcacga gccagtcacc ggccttcagc ttcacgtcgt
     1681 cgccgtcatc gtcgcccccc tcgaagcgca gcaccgccgc gcccgccagc agcgccaccc
     1741 actcgtcgcc agcctggtcg taccactcgc cgggcgcgct cgcatggccg tgcgagacga
     1801 tgcgctcgac acggcagcgc ggattggcga acagggtctg gaagatctcg ccgcctgcgg
     1861 gcggcggcag cgcggcgaag agattgccgc gcccggcagc ctggatctcg gcgctcattc
     1921 cccgctcacg aaacgatgta cgccgccgaa ccggtcgaca gcagcttgcc gtcggcgtcg
     1981 aggaagctca tccgcgtcga cgcgacgcgc gaacccaggc gcaggacctc ggcttccatg
     2041 cggaaggatt cgccgatacc ggggcgcagg tagtcgatgc gcaggtcgat ggtgccgagc
     2101 ttggagaagc ggtgcaggcg ctggaagggc ggttcgtcga ggtggcgggc gccgatcgcg
     2161 gccatcaccg cgagaccgcc catggcgtcg aggcaggcgg cgatcacgcc gccgtggatg
     2221 cggttatgca cgaaatgccc gacgagctcg tggcgcatcg cgatgcggcc atgcacgcgc
     2281 tcgccgccga tgtcgtcgat ctgcaggccg agcagcgcgt tgaaggcgat cttctgctcg
     2341 aagatctcgc ggatgccggc gatgaactcc ggctcgaact gcaccggcag ctccgccggg
     2401 gtggagtgtt tcggcatgga tgcatgctcc cttgcaagaa atggcgcagc cccgggcgtg
     2461 gcgggcacgc accggggcca cgggcaggct tgcgggcgcg gatacgcgcc gcagcctgca
     2521 gcgccccgca gggcgcgagg caacgatcag gcgcgcggca ccggcacgcc gggcgtcacg
     2581 cacagcgaat ggttgagggc gatgatcgcc ttcttgtagt cgctgcgctc gatgatgaac
     2641 tgcatgttca cctggcgcag ggtctgcgac acgcagttca cgttgatctg cgcgtcggcg
     2701 agcgcctgcg ccgcgcgtgc gagcacgccc gggatgctga tgttggagcc gatcgcgcac
     2761 acgatcgcgc tgttcttgac cgtcaccacc tggtagcgcg cctcgagctc ggcgatcagc
     2821 gcgggcgtca ccgagttctc ccacagcacg tgggcgatcg agttggcgtt ggtcgccttg
     2881 aggatgtagg agatgtcgtg cctgcagaag atctccatga tcccgaggtc gaagccgacg
     2941 gtgccgacca tgctggggtc gtggatctcg accagggtca ccttgtcgct gccggtcacc
     3001 atctcgacgc gggcgcgctc gccgacgtag tccttggtga tcagggtgcc cgggtgttcg
     3061 ggctcgaagg tgttcttgag gcggatcggg atgccggcga gctccatcgg cttggccgcc
     3121 ttggggtgga tcgcctccat gccgacgtcg gcgagctgat cggcgacgtc gtagttggtg
     3181 gcgcccacga tcaccgcgtt ctccaggccg acgatgttgg ggtcggccga ggagaggtgg
     3241 aactccttgt ggatcaccgc ctcggccggg cgcacctcga ccgcgatctt gctgaaggtg
     3301 acctcggaat agccgcgatc gaactcgcgc atgatgccct cggtgccctt ggtgtagccg
     3361 gtggcgacga tcacgctgtt gtcgaggtcg agccccttga agctgttgtg gatgcgctcg
     3421 tcgatggtcc acgccttgtc gtcgtggaag ccggcgaggt cgagcaggat ggcgttgacg
     3481 ccgttggcct tgaggatctc gacgctgttg aaggcgctgt gcgactcgcc caccgaggcc
     3541 agcacctcgc gcgcggcgag cagcacgtcc tggcggcgga tgtagccgct ggcgagcacg
     3601 tgctgcatcg cgtcgaggta cttcttgacc tcgccgatgc gggtgtcgac gaaggcgtcg
     3661 gcgacgtcca gcggcagggc gagctcggcg aagccggcgt tgagcttctt gaggctgacc
     3721 gcgagcgcgt cgagcgcgcc gtggtagtcg gaaccttccg cgaacagggc gtagatgccg
     3781 cgttcgccgg tcttcttgtg ctccagcagc tggttggtca cgccggaata ggccgacacc
     3841 acgtagatgc ggccgaggat gcgcgacttg tcgtagagca tgatgtggcg cagcacatcc
     3901 ccgaaggcgg acatcgaggt gccaccgatc ttttcaaccg agatcatccg ttttcccgag
     3961 ctttgcatga cgtgtgttcc agttctgatt agagaccgac cgaaacccgt catc
//
LOCUS       DAOQNN010000296         4014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14473_length_4014_cov_7.597120, whole genome shotgun sequence.
ACCESSION   DAOQNN010000296 DAOQNN010000000
VERSION     DAOQNN010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14473_length_4014_cov_7.597120"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="PKV97_13250"
     CDS             complement(<1..154)
                     /locus_tag="PKV97_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNB06892.1"
                     /translation="MSTAPQVAVIGGGYAGFAAAVTLARGGARFTLFEASRTLGGRAR
                     VVEKDGY"
     gene            complement(168..1001)
                     /gene="hpnD"
                     /locus_tag="PKV97_13255"
     CDS             complement(168..1001)
                     /gene="hpnD"
                     /locus_tag="PKV97_13255"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HNB06893.1"
                     /translation="MNPHDYCQDKAAKSGSSFYYSFMFLPAERRQAITALYAFCREVD
                     DVVDECHDLSLAQTKLEWWRQEVGRIYGGLPTHPVGHALKDVLKGFRLPQEQLLEIID
                     GMAMDLSQTRYLDFKGLQLYCYRVASVVGLLAAEIFGYQDRQTLKYAHDLGLAFQLTN
                     IIRDVGEDARRGRIYLPIEDLQRFKVPAKDLLEARYSDAFRELMAFQAERAEKFYDQA
                     FAQLPAIDRKAQRPGLVMAAIYRTLLREIARDGFLVLDRRTSLTPLRKIWLAGKTWFG
                     A"
     gene            complement(998..1846)
                     /gene="hpnC"
                     /locus_tag="PKV97_13260"
     CDS             complement(998..1846)
                     /gene="hpnC"
                     /locus_tag="PKV97_13260"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HNB06894.1"
                     /translation="MPVEHYENFPVASLLLPAHLREPVEAIYAFARGADDVADEGDAP
                     AVARLARLHDYQLGLEACAQGRPVADATLAPMFARLGRAIHTHALPLQPFRDLLDAFM
                     QDVGKTRYADFAELRDYCRRSADPVGRLMLHLYGAADAENLRRSDVICTALQLINFWQ
                     DVAVDWEKRRIYLPLDDLARFGLDEPALDALARGERSPGEDWRALMAFEVRRARTMML
                     DGAPLARTLRGRVGWELRLVVLGGLRILEGIEAVGYDVFHHRPALGRGDWARLGWRAL
                     NYGKLG"
     gene            2114..2791
                     /locus_tag="PKV97_13265"
     CDS             2114..2791
                     /locus_tag="PKV97_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB06895.1"
                     /translation="MLQLDDVTRILPGAPARVLFERLALRVKAGECVAIVGESGSGKS
                     TLLNCIAGLEPIDGGTIRVGETPIERLDDDAMARLRRSHFGFVFQAFHILPHLNLLQN
                     VALPLWLLGIAEAEAARRAQEMLSRVGLGARAPDPPQQLSGGELQRVAIARALVHRPH
                     LVLADEPTGNLDPGRASEVLDLLLGQIRDSRAAGVMVTHSRSAAARADRVLELRADGL
                     HDVTGHP"
     gene            2788..>4014
                     /locus_tag="PKV97_13270"
     CDS             2788..>4014
                     /locus_tag="PKV97_13270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB06896.1"
                     /translation="MSVPLRRIFLASLLRRRLASALSLLAIALGVALGLAVHLIHGAA
                     LDEFGRGMRAVAGKADLQVVGPRDGFDEALFATLARRPEVAAASPVLEIEARLPGREE
                     SLRILGVDLFRVLRVQPGLMPLAEGGGDLRFAALRPGVVFLSDDARQRLSAPGRSMEG
                     EVLPPALVDARIAVQSGVRSLVLEASGRVAGAGGVLGVMDLGAAQQAFARIGVLSRID
                     LRLAEGVSPAAAQAALSALLPPGIALRTPEAAAGELGSLSRAYRVNLTMLAAIALLTG
                     GFLVFSTQLLSVARRRREFALLRALGLVRAELMDQEHQRCVRSGQTFCLVKLDIDGFK
                     RVNDAHGQAIGDAVLRALAQEALRHVRGSDLVGRWSGEEFVVMLPDTRAVLARAPMER
                     LVQRLAALRILHGAEAV"
BASE COUNT          555 a         1387 c         1484 g          588 t
ORIGIN      
        1 ggtagccgtc cttctcgacc acccgcgccc ggccaccgag ggtgcgcgaa gcttcgaaca
       61 gcgtgaaccg cgcgccgccg cgggcgaggg tgacggccgc cgcaaagccg gcgtagccgc
      121 cgccgatgac agccacctgg ggcgcggtgc tcatcgcgcg ataccgctca tgccccgaac
      181 cacgtcttgc cggcgagcca gatcttgcgc agcggggtga gggaggtgcg gcggtcgagc
      241 accaggaaac cgtcgcgcgc gatttcgcgc agcagggtgc ggtagatcgc cgccatcacc
      301 aggccggggc gctgcgcctt gcggtcgatc gcgggcaact gggcgaaggc ctggtcgtag
      361 aacttctccg cgcgctcggc ctggaaggcc atgagctcgc ggaaggcgtc ggagtagcgc
      421 gcctcgagga ggtccttggc gggcaccttg aagcgctgca ggtcctcgat cggcaggtag
      481 atgcgcccgc ggcgggcgtc ctcgccgacg tcgcggatga tgttggtgag ctggaaggcc
      541 agcccgaggt cgtgcgcata cttgagcgtc tggcggtcct ggtagccgaa gatctcggca
      601 gcgagcaggc cgacgacgct ggcgacgcgg tagcagtaga gctgcaggcc cttgaagtcg
      661 aggtagcgcg tctgcgacag gtccatcgcc atgccgtcga tgatctcgag cagctgctcc
      721 tgcggcaggc ggaagccctt cagcacgtcc ttgagcgcat gtccgacagg gtgggtgggc
      781 aggccgccgt agatgcgccc gacctcctgg cgccaccact cgagcttggt ctgcgcgagc
      841 gagaggtcgt ggcattcgtc gaccacgtcg tccacctcgc ggcagaaagc gtacagggcg
      901 gtgatcgcct ggcggcgctc ggcgggcagg aacatgaagc tgtagtagaa gctcgagccg
      961 ctcttggcgg ccttgtcctg gcagtagtcg tgtggattca tccgagtttt ccgtaattca
     1021 gcgcgcgcca gcccaggcgc gcccagtcgc cgcgaccaag cgcggggcgg tggtggaaga
     1081 catcgtagcc gaccgcctcg atcccttcga gaatgcgcag cccgcccagc acgaccagtc
     1141 gcaactccca gccgacgcgc ccgcgcaggg tacgggcgag cggggcgccg tcgagcatca
     1201 tcgtgcgagc acgccgcacc tcgaaggcca tcagcgcgcg ccagtcctcg cccgggctgc
     1261 gttcgccgcg ggcgagcgcg tcgagcgcgg gctcgtcgag gccgaagcgg gcgagatcgt
     1321 cgagcggcag gtagatgcgc cgcttttccc agtcgaccgc gacgtcctgc cagaagttga
     1381 tcagctgcag ggcggtgcag atcacgtccg agcggcgcag gttctccgcg tccgccgcgc
     1441 catacagatg cagcatcagg cgccccaccg ggtcggccga gcgccggcag tagtcgcgca
     1501 gctctgcgaa gtcggcgtag cgcgtcttgc cgacgtcctg catgaaggcg tcgagcaggt
     1561 cgcggaaagg ctgcagcggc agcgcatggg tatggatcgc gcggccgagg cgggcgaaca
     1621 tcggcgccag cgtcgcgtcg gccaccggcc ggccctgcgc gcaggcctcg agcccgagct
     1681 ggtagtcgtg caggcgcgcc aaccgcgcca ccgcgggcgc gtcgccttcg tcggcgacgt
     1741 cgtcagctcc gcgcgcgaag gcatagatcg cctccaccgg ctcgcgcagg tgggccggca
     1801 acagcagtga ggcgacaggg aagttctcgt agtgctcgac cggcatcggg gcaaaggagc
     1861 ggcggaatcc gcagggtgcc gcagtatatc cgcacgcctc gtggagaagc ggcgcaagcc
     1921 gcgaacaggg cggttcgcca atgggggctt cgcaatctcc accgccgcat tcgccccttg
     1981 cggcgctcct accggaggca tcgcacccgg aagcgcatgc aggagcggcg caagccgcga
     2041 accttttgcg cagggcaatg acacaatggc gtccatcatt ccgctctcca gccccgtgcc
     2101 acgaggcctt ccgatgctgc agctcgacga cgtcacccgc atcctgcccg gcgcccccgc
     2161 gcgcgtgctg ttcgagcgcc tggcgctgcg ggtcaaggca ggggagtgcg tggcgatcgt
     2221 cggcgagtcg ggctcgggca agtcgaccct gctcaactgc atcgccggcc tggaaccgat
     2281 cgacggcggc acgatccggg tcggcgaaac acccatcgag cgcctcgacg acgacgccat
     2341 ggcgcgcctg cgccgtagtc acttcggttt cgtgttccag gctttccaca tcctgcccca
     2401 cctgaacctg ctgcagaacg tggcgctccc cttgtggctg ctcggcatcg cggaggccga
     2461 ggccgcgcgc cgcgcacaag agatgctcag ccgcgtcggg ctgggcgcgc gcgcgccgga
     2521 tccgccgcag caactgtccg ggggtgagct gcagcgcgtg gcgatcgcgc gcgcgctggt
     2581 acatcggcct cacctggtgc tcgcggacga gcccaccggc aacctcgacc cggggcgagc
     2641 gagcgaggta ctcgacctgc tgctcggcca gatccgcgac agccgcgccg ccggcgtcat
     2701 ggtcacgcac tcgcgcagcg cggcggcgcg cgccgaccgc gtgctggaac ttcgagccga
     2761 cgggctgcat gacgtcacgg ggcatccgtg agcgtgcctc ttcgacgcat cttcctggcc
     2821 agcctgctgc gccgccgcct ggcgagcgcc ctgtcgctgc tggcgatcgc gctcggcgtg
     2881 gcgctggggc tggcggtgca tctgatccac ggtgccgcgc tcgacgagtt cggccgcggc
     2941 atgcgcgcgg tggcgggcaa ggccgacctg caggtggtgg ggccgcgcga cgggttcgac
     3001 gaggccttgt tcgccaccct ggcgcgccgg cccgaagtgg cggcggcgag tccggtgctg
     3061 gagatcgagg cgcgcctgcc cgggcgcgag gagagcctgc gcattctcgg cgttgacctc
     3121 ttccgcgtgc tgcgcgtgca gcccgggctg atgccgctgg cagagggcgg cggggacctg
     3181 cgcttcgcgg cactgcgtcc cggcgtggtg ttcctgagcg acgacgcccg gcagcgcctt
     3241 tccgcgccgg ggcgctccat ggaaggcgag gtgctgccgc cggcgctcgt cgacgcccgc
     3301 atcgcggtgc agtcgggcgt gcgcagcctg gtgctcgagg cgagtgggcg ggtggcgggc
     3361 gcaggcggcg tgctcggcgt catggacctc ggcgcagcgc agcaggcctt cgcacgcatc
     3421 ggcgtgttga gccggatcga cctgcgcctg gccgagggcg tttccccggc ggcggcgcag
     3481 gcggccttgt ccgccctgct gccaccgggg atcgccttgc gcacacccga ggcggcagcg
     3541 ggcgagctcg gctcgctgtc gcgcgcatac cgggtgaacc tgaccatgct ggcggcgatc
     3601 gcgctgctga ccggcggctt cctcgtgttc tccacgcagc tgctgtcggt ggcgcggcgg
     3661 cggcgcgagt tcgcgctgct gcgcgcgctc ggtctggtgc gcgccgagct gatggaccag
     3721 gagcaccagc gctgcgtgcg ctcgggccag accttctgcc tggtcaagct cgacatcgac
     3781 ggcttcaagc gcgtcaacga cgcgcatggc caggcgatcg gcgacgccgt gctgcgtgcg
     3841 ctcgcgcagg aggccttgcg gcacgtgcgc ggctcggatc tcgtcggccg ctggagcggc
     3901 gaggagttcg tcgtcatgct gcccgacacg cgcgccgtgc tcgcgcgcgc gccgatggag
     3961 cggctcgtgc agcggctggc cgcgctgcgc atcctgcacg gcgccgaagc ggtg
//
LOCUS       DAOQNN010000297         3997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14587_length_3997_cov_8.135211, whole genome shotgun sequence.
ACCESSION   DAOQNN010000297 DAOQNN010000000
VERSION     DAOQNN010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14587_length_3997_cov_8.135211"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKV97_13275"
     CDS             complement(<1..282)
                     /locus_tag="PKV97_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNB06897.1"
                     /translation="MHTHHGHQHDHHAHNEHDHQHGDPAHGHAYPHQAGHAHPPHHAH
                     DHSASRFLPLALVLTLGFAAVEAIAGWWSGSLALLGDAGHMLTDAASLGL"
     gene            complement(391..1707)
                     /locus_tag="PKV97_13280"
     CDS             complement(391..1707)
                     /locus_tag="PKV97_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB06898.1"
                     /translation="MFNRKLKSTLQEQQAELAARQAVIEAIDRATARIEFAPDGTVLF
                     ANELFLATMKYRSEEVLGQHHRMFCDEAYANSSDYLAHWQRLRRGEPVSGRFERRDAT
                     GEPVWLEATYNPVFGPDGRVLRVVKLATDITAQVREGAEHRAMLSAIERSMAMIEFDL
                     DGKVLAANDNFLATMGYARHEVLGRHHRLFCAAEDAATPEYAQFWERLRHGEYFSGRF
                     RRVARNGRTVWLEASYNPVFDASGRPCKFIKLAADISPRVEALEEEVANAAAALERAR
                     DNAHLAERGTDVIARATAQMVAISNSAEDAAQTFGQLGDETGRITTIVNTIREIADQT
                     NLLALNAAIEAARAGEHGRGFAVVADEVRKLAERTSKATTEISAMISTVQDATHLAIS
                     RVHGMHETATASIGLAREAGEVIDGIHNGATEVVEVVERFSGLLHR"
     gene            1874..2779
                     /locus_tag="PKV97_13285"
     CDS             1874..2779
                     /locus_tag="PKV97_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNB06899.1"
                     /translation="MKKSIGQRQRGIRLLSWNIQWGRGADGRVDLARTRAAIRAAGPL
                     DLICLQEVASNFPGLAGGAREDEPAFFAEACADFEPVFGAGMDVPDGDGGRARFGNLL
                     LSRLPVGQVFRHLLPSPADPACPAMQRVCVEAVVEAPWGPLRVMSTHLEYYSARQRRA
                     QIEALRALQAQAADLARLPAAGKESNPAFAARPRPASAVLCGDFNCEPGSAEYLALQR
                     PLPASELAPDAERPHWVDAWAVAHGARAHAPSVGLHGAEWPDRSYCCDFFFVTADLAP
                     RVCAVEVLADTAASDHQPVVLELGA"
     gene            complement(2822..3613)
                     /locus_tag="PKV97_13290"
     CDS             complement(2822..3613)
                     /locus_tag="PKV97_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB06900.1"
                     /translation="MTQTLQLDGRRAAFDESGPAAAPAILLIHGAGHDRGVWQAVTAG
                     LAAAGRRVIVPDLPGHGDSEGEAPGAVDTMAAWVLAFADALGLERFELAGHSMGSLVA
                     LAAAAAAPGRIARLHLLGSLAPMPVAPFLLDAARSDPAQAHALINKFSFAPAEVLGEE
                     RRRMLEAGNRERMERNRSETLARDLAACNTWQDGLVAAARRDCPTLLLCGALDRMTPI
                     EAIGPLHEALRAGGGEVRLVELAGCGHAMQEEAPTAVVRALLGTD"
     gene            complement(3770..3985)
                     /locus_tag="PKV97_13295"
     CDS             complement(3770..3985)
                     /locus_tag="PKV97_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HNB06901.1"
                     /translation="MTGESFDEVTSLRQRLDELRLEHRDLDDAIARLADNPVEDELML
                     RRLKKRKLALKDRIVAIEHLLDPDEYA"
BASE COUNT          488 a         1296 c         1581 g          632 t
ORIGIN      
        1 cagcccgagc gaggccgcgt cggtgagcat gtggccggcg tcgccgagca gggcgagcga
       61 gcccgaccac catccggcga tcgcctcgac cgcggcgaag ccgagcgtga gcacgagcgc
      121 gagcggcagg aagcggctcg cggagtggtc gtgcgcgtga tgaggggggt gggcgtgacc
      181 ggcttgatgc gggtatgcgt ggccgtgtgc ggggtcgccg tgttggtggt cgtgctcgtt
      241 gtgggcgtgg tggtcgtgct gatgaccgtg atgggtgtgc atcgcagcgc ttcctcgagg
      301 gcttcagcgg attatgcctg tggcgcagtc ggtcccgaca cctccggcat ccgcggcggg
      361 ccgcggcgcg cgtccacacc cttcggggac tcagcgatgc agcagccccg agaagcgctc
      421 caccacctcg acgacctcgg tggcgccgtt gtggatgccg tcgatgacct cgccggcctc
      481 gcgtgccaag ccgatgctcg cggtggcggt ctcgtgcatg ccgtgcaccc gcgagatcgc
      541 caggtgggtg gcgtcctgca cggtggagat catggcgctg atctcggtgg tggccttgga
      601 ggtgcgctcg gcgagcttgc gcacctcgtc ggcgaccacc gcgaagccgc ggccgtgctc
      661 gccggcgcgg gcggcctcga tcgccgcgtt gagcgcgagc aggttcgtct ggtcggcgat
      721 ctcgcggatg gtgttgacga tggtggtgat gcgcccggtc tcgtcgccga gctggccgaa
      781 ggtctgcgcg gcatcctcgg cgctgttcga gatcgccacc atctgtgcgg tggcgcgcgc
      841 gatcacgtcg gtgccgcgct cggcgaggtg ggcgttgtcg cgggcgcgtt cgagcgcggc
      901 ggcggcgttg gcgacctctt cctcgagcgc ctcgacgcgg gggctgatgt cggcggcgag
      961 cttgatgaac ttgcagggcc gaccggacgc atcgaacacc gggttgtagc tggcctcgag
     1021 ccagaccgtg cggccgttgc gcgccacgcg gcggaagcgg ccggagaaat attcgccgtg
     1081 gcgcaggcgc tcccagaact gcgcgtactc gggcgtcgca gcgtcctcgg cggcgcagaa
     1141 caggcgatgg tggcggccaa gcacctcgtg gcgggcgtag cccatggtgg cgaggaagtt
     1201 gtcgttggcg gcgaggacct tgccgtcgag gtcgaattcg atcatcgcca tcgagcgctc
     1261 gatcgccgac agcatggcgc ggtgctcggc gccctcgcgg acttgggcgg tgatgtcggt
     1321 ggcgagcttg accacgcgca gcacgcggcc gtcggggccg aatacggggt tgtaggtggc
     1381 ctcgagccac accggctcgc cagtggcgtc gcggcgctcg aagcgcccgc tcaccggctc
     1441 gccgcggcgc aggcgctgcc agtgcgcgag gtagtccgac gaattcgcgt aggcctcgtc
     1501 gcagaacatg cggtggtgct gcccgagcac ctcttcgctg cgatacttca tggtcgcgag
     1561 gaagagctcg ttggcgaaca gcacggtacc gtcgggtgcg aactcgatgc gggcggtggc
     1621 gcggtcgatc gcctcgatca ccgcctggcg tgcggcgagc tcggcctgtt gttcctggag
     1681 ggtgctcttg agcttgcggt tgaacatggt ctggtggccg tcgtcgggag tcgcgatgac
     1741 gggcggccgc aggccgcctg tccggatgcg cggcgtgtcg ggctggaagg cggcggggcg
     1801 tccgcgccgg acacggggcg tgcccggtat aacggcgcgc gggggtcttt ctttacatca
     1861 tgtcgaaatg cgtatgaaga aatcgatcgg gcagcgacaa cgaggcattc gcctgctgag
     1921 ctggaacatc cagtgggggc gcggggcgga cgggcgggtg gatctggcgc gtacgcgcgc
     1981 ggcgatccgc gccgccgggc cgctcgacct gatctgcctg caggaagtgg cgagcaattt
     2041 tcccggcctc gccggcggtg cgcgcgagga cgagccggcc ttcttcgccg aggcctgtgc
     2101 ggatttcgag ccggtgttcg gcgcgggtat ggacgtgccc gacggcgacg gcgggcgggc
     2161 gcgcttcggc aacctgctgc tgtcgcgcct gccggtgggg caggtgttcc gccacctgct
     2221 gccgtcgccc gccgaccccg cctgtccggc gatgcagcgg gtgtgcgtgg aggcggtggt
     2281 ggaggcgccc tgggggccgc tgcgggtgat gagcacccac ctcgagtatt actccgcgcg
     2341 ccagcgccgc gcccagatcg aggccttgcg cgcgctgcag gcccaggcgg cggacctggc
     2401 gcggttgccg gcggcgggca aggagtccaa ccccgccttc gccgcgcgcc cgcgtccggc
     2461 ctcggcggtg ctgtgcgggg acttcaattg cgaaccgggc tcggccgaat acctcgcgct
     2521 gcagcgtccg ctacccgcgt ccgagctcgc accggacgcc gagcgcccgc actgggtgga
     2581 cgcgtgggcg gtcgcgcacg gcgcgcgagc gcatgcgccc agcgtcgggc tgcacggtgc
     2641 ggagtggccc gaccgcagtt attgctgcga cttcttcttc gtgactgccg acctcgcgcc
     2701 gcgggtgtgc gcggtcgagg tgctcgcgga caccgcggcc tcggatcacc agccggtggt
     2761 gctggaactg ggcgcgtgag acgagaggag cgcggcaggg ctgacgaccg cccggccggg
     2821 ctcagtcggt cccgagcagg gcccgcacca cggcggtcgg ggcctcttcc tgcatcgcgt
     2881 ggccgcagcc tgccagctcc accagcctca cttcgccgcc gccggcgcgc agggcctcgt
     2941 gcagcgggcc gatcgcctcg atcggggtca tgcggtcgag ggcaccgcac agcagcaggg
     3001 tgggacagtc gcggcgcgcg gcggcaacca gcccatcctg ccaggtgttg caggcggcga
     3061 ggtcgcgcgc gagcgtctcg ctgcggttgc gttccatgcg ctcgcggttg ccggcctcga
     3121 gcatgcgccg gcgttcctcg ccgaggactt cggcgggggc aaaggagaac ttgttgatca
     3181 gcgcgtgggc ctgcgcaggg tcgctgcgcg cggcatcgag caggaagggc gcgaccggca
     3241 tcggcgcgag gctgccgagc aggtgcaggc gtgcgatgcg ccccggcgcg gcagccgcgg
     3301 cggccagcgc gaccagcgag cccatgctgt gcccggcgag ttcgaagcgt tcgaggccga
     3361 gcgcgtcggc gaaagcgagc acccatgctg ccatggtgtc gaccgcgccg ggggcctcgc
     3421 cttccgaatc gccgtgtccg gggaggtcgg ggacgatcac ccggcggccg gcggcggcca
     3481 gcccggcggt gaccgcttgc cagacgccgc ggtcgtgtcc ggcgccgtgg atcagaagga
     3541 tggccggcgc ggcggcgggg ccggactcgt cgaaggcggc gcggcggccg tcgagctgga
     3601 gggtctgggt catcggcgtg ttcttggagg gttcgggacc gcggacgggc gcaggcgtcc
     3661 tggagggggc tctgcgaagc cgagcctgcc gcgtgcgggt tcaggcggcc ggacgttcag
     3721 ccggccggat ggagtgtcga gccggcgggt tgttcaggca ccgtggcagt caggcatatt
     3781 cgtcggggtc gagcaggtgc tcgatcgcga cgatgcggtc cttgagtgcg agcttgcgtt
     3841 tcttgagccg gcgcagcatc agctcgtcct ccaccgggtt gtcggcgagg cgggcgatcg
     3901 cgtcgtcgag gtcgcggtgc tcgaggcgca actcgtccag ccgctggcgc aggctggtca
     3961 cctcgtcgaa gctctccccg gtcatgctcg ttcccct
//
LOCUS       DAOQNN010000298         3991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14623_length_3991_cov_5.341717, whole genome shotgun sequence.
ACCESSION   DAOQNN010000298 DAOQNN010000000
VERSION     DAOQNN010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14623_length_3991_cov_5.341717"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1896
                     /locus_tag="PKV97_13300"
     CDS             58..1896
                     /locus_tag="PKV97_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB06902.1"
                     /translation="MKIRVSALASACALACFLAPAAHAADAQLGHVVVTATRQPISAD
                     AALASVDVIERDEIARAGHSSLLGLLSSRPGVQMARNGGPGSSGSIFIRGANSGHTLV
                     LVDGVRMGSATSGSPVLETIPLELIERIEILRGPGSALYGADALGGVIQVFTRKGREG
                     FQPSVRVGAGTDGAREASATLAGGNERLRYSLTAGHERSDGFNARPNSATAADADDDG
                     FREDYLGASLVLDLGGNDELGANLLYSDMRNWYDAAQPFDSYLDKRAETFGAYLRKQH
                     SADWASTLRFGHGVDALDNQSNASTRSRFDTTQRQLSWQHDVAVGGGSLMAAYEFLQQ
                     RVKTTSDFEKTRRHINAFLLGWGGEFDRHNVQLNARHDRNSQFGGKTTGAAAYGYRLA
                     PEWRAHASIGTAFKAPTFNDLYFPVECYGAWGCFGGNPDLEPEEALNRELGVAWERNG
                     VGVDLTYFNNRIKNLIDWSTGIASNVGRADIQGIEAALSATLGDYRLRASVDLLDAED
                     DETGDQLGRRARVGGALALERAVGAWTWGVEWNGKGRRYDQVPNAVSNRLGGYGLVDA
                     YAHYAVARDWSVEVRANNLLDKDHELAKGFATQGRSAFVALRYAMH"
     gene            1948..2934
                     /locus_tag="PKV97_13305"
     CDS             1948..2934
                     /locus_tag="PKV97_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNB06903.1"
                     /translation="MRRRRAPLAIAAVLLSAAAMALLWALVAGDLPVAATDVFAALAG
                     AEGMGATVIRELRLPRALAAFACGGLLATAGALMQVLLRNPLADPYILGISGGAAVGA
                     LGAMMLGAALFWVDVSAFAGALAAMLLVFGLAHGDGSWTQTRLLLTGVIVAAGCGAAV
                     SLMLALAPDTRVMSMLFWMMGDASAAARPWPALAVLALALVAVIPFARDLNVLARGEL
                     SARALGVRVGHLRVALYVLASLLTATAVTLVGSVGFVGLVVPHLVRLALGNDQRVLIP
                     AAALAGGTLLTLADTAARTVMAPQQLPVGVLTALIGVPVFLFLLSRNPRGTP"
     gene            2931..3740
                     /locus_tag="PKV97_13310"
     CDS             2931..3740
                     /locus_tag="PKV97_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB06904.1"
                     /translation="MNAARRALPVDDAPLLEAERLALEVGERWLCCEFSLSLAAGECL
                     VLLGPNGAGKTTLLHTLAGLREPTVGEVRIGGLPYAAWPALEAARFRGLLAQQQPDPF
                     AASVLETVLVGRHPHLGRWGWEGPQDVAIARAALESVGLSGFEQRDVQTLSGGERQRV
                     GIATLLAQAPRLFLLDEPLNHLDLHHQIAALELFQRLLREQGTARGSVLVLHDINLAA
                     RYADHIILLDGRGGVIAGDRDAVLRADLLGQAFGHPLRRIEHEGRVVFLPD"
BASE COUNT          525 a         1410 c         1437 g          619 t
ORIGIN      
        1 atgtgaggtt gccatggcgt gcggggacgc gcggcatggc gaagggagtt caaaccaatg
       61 aagatccgtg tttccgcgct cgcctcggca tgcgcgctcg cctgtttcct cgcaccggcc
      121 gctcatgcgg ccgatgccca gctcggccac gtcgtcgtga ccgccactcg ccagccgatc
      181 agtgccgatg cggccctggc cagcgtggat gtcatcgagc gcgacgaaat cgcccgcgcg
      241 gggcattcga gcctgctggg cctgctctcc tcgcggccgg gcgtgcagat ggcacgcaat
      301 ggcggtcctg gttcgagcgg cagcatcttc atccgcgggg cgaactccgg acacacgctg
      361 gtgctggtcg atggggtgcg catgggctcc gccaccagcg gctctccggt gctggagacg
      421 atcccgctcg aactcatcga gcgtatcgag atcctgcgcg gccccggcag tgcgctgtac
      481 ggcgcggatg cgctcggtgg cgtgatccag gtgttcaccc gcaaggggcg cgagggcttc
      541 cagccctccg tgcgcgtcgg ggccggcacg gacggcgcgc gcgaggcgag cgccacgctc
      601 gcgggtggaa acgagcgtct gcgctacagc ctgaccgccg gtcacgagcg cagcgacggc
      661 ttcaacgcac ggccgaattc ggcgaccgcc gcggatgccg acgacgacgg cttccgcgag
      721 gattatctgg gcgcgtcgct ggtgctggac ctgggcggca atgacgagct cggcgccaac
      781 ctgctgtatt ccgacatgcg caactggtac gacgcggcac agcctttcga ctcctacctc
      841 gacaagcgtg ccgagacctt cggcgcgtat ctgcgcaagc agcattccgc cgactgggcc
      901 agcaccctgc gcttcggtca cggggtggat gccctcgaca accagtcgaa cgcgagcacg
      961 cgttcccgct tcgataccac gcagcgccag ttgagctggc agcacgacgt cgccgtaggt
     1021 ggcggctcgc tgatggcggc gtacgagttt ctgcagcagc gcgtgaagac cacctccgac
     1081 ttcgagaaga cgcgtcgcca catcaacgcc ttcctgctcg gctggggtgg tgaattcgac
     1141 cgccacaacg tgcagctcaa tgcgcgccac gaccgcaact cgcaattcgg tggcaagacc
     1201 accggcgccg cggcctacgg ctatcggctt gcgcccgagt ggcgcgccca tgccagcatc
     1261 ggcaccgcgt tcaaggcgcc gaccttcaac gatctgtact tcccggtgga gtgctacggt
     1321 gcctggggct gcttcggcgg caatccggac ctggagcccg aggaggcgct caaccgtgag
     1381 ctgggcgtgg cctgggagcg caacggggtc ggtgtcgacc tgacctattt caacaaccgc
     1441 atcaagaacc tcatcgactg gagcaccggc atcgcctcca atgtgggcag ggccgacatc
     1501 caggggatcg aggcggcgct gtccgcgacc ctgggcgact accggctgcg cgccagcgtc
     1561 gacctgctcg acgcggagga tgacgagacc ggcgatcagc tcggccgccg cgcgcgcgtc
     1621 ggcggggcgc tcgcgctgga gcgtgcggtg ggcgcgtgga cctggggtgt ggagtggaac
     1681 ggcaaggggc ggcgctatga tcaggtgccg aacgcggtgt ccaatcgcct cggcggctac
     1741 ggtctggtcg atgcctacgc gcactacgcc gtggcgcgcg actggagcgt cgaagttcgt
     1801 gccaacaatc tactcgacaa ggaccatgag cttgcgaagg ggttcgcaac acagggcagg
     1861 agtgccttcg tcgcgctgcg ctacgcgatg cactgaccgg cgatgcccgc cgtcgccctg
     1921 cgtggggagt cgcgcttgag tccggccatg cgccgcaggc gggcgccgct ggcgatcgct
     1981 gcggtactgc tgtccgcggc ggccatggcc ttgctgtggg cgctggtcgc cggcgacctg
     2041 ccggttgcgg cgaccgatgt cttcgccgcg ctggccggcg cggagggcat gggggcgacc
     2101 gtcatccgcg aactccgcct cccccgcgcc ctcgccgcct tcgcctgcgg cggcctgctc
     2161 gccaccgcgg gtgcgctgat gcaggtgctg ctgcgcaacc cgctcgccga cccctacatc
     2221 ctgggcatct cgggcggggc ggcggtgggg gcgctggggg cgatgatgct cggggcggcg
     2281 ctgttctggg tggatgtgtc ggccttcgcc ggggcgctgg cggcgatgct gctcgtcttc
     2341 gggctcgccc atggcgacgg cagctggacg cagacgcgcc tgctgctcac cggcgtcatc
     2401 gtcgccgccg gctgcggcgc ggcggtgtcg ctgatgctgg cgctcgcgcc cgacacgcgg
     2461 gtgatgtcga tgctgttctg gatgatgggc gacgccagcg cggcggcgcg gccgtggccg
     2521 gcgctggcgg tgctggcgct ggcgctggtc gcggtgatac ccttcgcgcg cgacctcaac
     2581 gtgctcgcgc gtggcgagct cagcgcgcgc gcgctgggcg tgcgcgtcgg ccacctgcgc
     2641 gtcgcgctct atgtgctcgc ctcgctgctc accgccaccg cggtcacgct cgtgggctcg
     2701 gtgggcttcg tcggcctggt ggtgccgcac ctggtgcggc tggcgctggg caacgaccag
     2761 cgcgtgctga tccccgcggc ggcgctcgcc ggcggcaccc tgctgaccct ggccgacacc
     2821 gcggcgcgca ccgtgatggc gccgcagcag ctgccggtgg gcgtgctcac cgcgctgatc
     2881 ggcgtgccgg tcttcctctt cctgctctcg cgcaacccgc gcggcacgcc atgaacgccg
     2941 cccgccgcgc cttgcccgtg gatgacgccc ccttgctcga ggccgagcgc ctcgcgctcg
     3001 aggtgggcga gcgctggctg tgctgcgagt tcagcctgag cctggcggcg ggcgagtgcc
     3061 tggtgctgct cggccccaac ggcgcgggca agaccacgct gttgcacacc ctggccggac
     3121 tgcgcgagcc cacggtgggc gaggtgcgca tcggcggcct gccctatgcg gcctggccgg
     3181 cgctggaggc ggcgcggttc cgcggcctgc tcgcgcagca gcagcccgac cccttcgcgg
     3241 ccagcgtgct cgagaccgtg ctggtcggcc gccacccgca cctggggcgc tggggctggg
     3301 aggggccgca ggacgtggcg atcgcgcgcg cggcgctgga gtcggtcggg ctgagcggct
     3361 tcgagcagcg cgacgtgcag acgctttcgg gcggcgagcg ccagcgcgtg ggcatcgcca
     3421 ccctgctcgc gcaggcgccg cgcctgttcc tgctcgacga gccgctcaac cacctcgacc
     3481 tgcaccacca gatcgccgcg ctcgagctct tccagcgcct gctgcgcgag cagggtaccg
     3541 cgcgcgggag cgtgctggtg ctgcacgaca tcaacctcgc cgcacgctac gccgaccaca
     3601 tcatcctgct cgacggccgt ggcggcgtga tcgccggcga tcgcgacgcc gtgctgcgtg
     3661 ccgacctgct cggccaggcc ttcggccacc cgctgcgccg gatcgagcac gaggggaggg
     3721 tggtgttcct gccggactga gcgcagggag cgggcaggag agcgtatcgg tgtctccgat
     3781 gttccgcgtt catcggcttt cccgatagcg gacgcgttcc gatgcgggtg caggctcccg
     3841 ccgcatcgtg acgccttccg caccatcctc ccgcggcgct cacgccatcg ccgccgggcc
     3901 cccgaaaacg aacaggaaga aacgacatga cccactcccc cgaccgcgac acccgccaca
     3961 acgcccgcat ggcacgcaag aaggacgtcg t
//
LOCUS       DAOQNN010000299         3985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14675_length_3985_cov_9.637913, whole genome shotgun sequence.
ACCESSION   DAOQNN010000299 DAOQNN010000000
VERSION     DAOQNN010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14675_length_3985_cov_9.637913"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKV97_13315"
     CDS             <1..190
                     /locus_tag="PKV97_13315"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HNB06905.1"
                     /translation="DEKWVTERAYDNPKFVEDLVRDIALRLRADARVASWRVQSENFE
                     SIHNHSAYALVEGENAPE"
     gene            196..1569
                     /locus_tag="PKV97_13320"
     CDS             196..1569
                     /locus_tag="PKV97_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB06906.1"
                     /translation="MKMGEIERLHATGEAGRGAEGRRIAVIGGGIAGLSTAWLLAPQH
                     SVTLFEAGSYVGGHTNTIDVTVDGLTHPVDTGFLVFNRRTYPNLCALFALLQVEAVET
                     EMSFGVSLAEPELEWAGTDLASVFAQRGNLVRPAFLAMLRDILRFNRETTRMASEGGM
                     PALSLGEYLDLEDYSQAFRDWYLLPMAAAIWSCPTRTMLAYPLATFVQFCHNHGLLQI
                     LDRPTWMTVKGGGRSYVRRMLARLDDVRVNAPVQRVVRGEDGVWVHTAAGPERFDEVV
                     FACHSDQALAILGSEATPDERRILGAVRYQANVAYLHTDTALLPRRPKVWSAWNYLAG
                     KGAPDARPVSVSYLINRLQPLPFQTPVVVSLNPFIAPAADKTFRRIEYAHPVFDHGAI
                     DAQAALPSIQGRERCWFAGAWTGYGFHEDGLKSAVAVVEAMGVEVPWRRGRVPAAKRA
                     GTALEAA"
     gene            1569..2339
                     /locus_tag="PKV97_13325"
     CDS             1569..2339
                     /locus_tag="PKV97_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNB06907.1"
                     /translation="MPDALQPAVCFGAVMHERHVQAHNRFVYPSAFLRLPLGRLDQVR
                     APLLGIERANLFSFRSRDHGARDGSPLLPWIRALLNERGLAAACDGEIVLQTMPRILG
                     FVFNPVSFWFCHDRAGGLRAVLAEVNNTFGERHNYLLHHADLRPIGAGDELRASKRFH
                     VSPFFPVRGEYRFRFEQRGAVHAVSIELWDGGERQLSTRLAGRAEAVGGASMAKWLAR
                     QPFMTLGVVARIHWQALRLALRRVSFFRKPAPPLEETT"
     gene            2339..3580
                     /locus_tag="PKV97_13330"
     CDS             2339..3580
                     /locus_tag="PKV97_13330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNB06908.1"
                     /translation="MNALDHALQPLAGLRLARLPRATRLCLEMLDRLDGGAIAVELPD
                     GVRVRAGHGALVAHLRVRDHAVFDEALARGDIGFGEAWMDGLWDTEDLPGLLRLLSAN
                     RSRLQGAIYGRMFRLLSHRLQHLLRANTRSGSRRNIEAHYDLGNDFYALWLDPTMTYS
                     AAVFASPDESLQDAQLRKYRRILGELGARPGQTILEVGCGWGGFAEVAATEFGCKVLG
                     ITLSPAQLAFARARAERGGFADRVDLELCDYRDVRGQYDHVVSIEMIEAVGERFWPTY
                     FAQLSALLKPGGRCVVQAITIADALFARYRRGTDFIQRHVFPGGMLPSPGAVAQQAGR
                     AGLEVVGDFAFGRDYARTLAHWHRSFDAQAAAVRAQGFPERFLRMWRFYLAYCEAGFD
                     TGDLDVHHYVFAHAAAGSQGG"
     gene            3666..>3985
                     /locus_tag="PKV97_13335"
     CDS             3666..>3985
                     /locus_tag="PKV97_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06909.1"
                     /translation="MSAPICLPARSPRRLQRSFERFARLPRALGAVLACCMLLALETV
                     HALGLPPQLVASTAGLREVGRGELRWLGFKLYDAALWARAPGEQATADEHVLSIRYAR
                     AVTSA"
BASE COUNT          516 a         1480 c         1385 g          604 t
ORIGIN      
        1 ggacgagaag tgggtcaccg agcgcgccta cgacaacccc aagttcgtcg aggacctcgt
       61 gcgcgacatc gcgttgcgcc tgcgtgccga tgcgcgcgtg gcctcgtggc gggtgcagtc
      121 ggagaacttc gagtccatcc acaaccattc cgcctacgcc ctcgtcgagg gcgagaacgc
      181 gccggagtaa tcgccatgaa gatgggtgag atcgagcgcc tgcacgccac gggcgaggcg
      241 ggccggggtg ccgaggggcg gcggatcgcc gtgatcggcg gcggcatcgc cggtctgtcg
      301 accgcctggc tgctcgcgcc gcagcacagc gtgacgctgt tcgaagcggg cagctacgtc
      361 ggcggtcaca ccaacaccat cgacgtgacc gtcgacggcc tcacccatcc ggtggacacc
      421 ggcttcctgg tcttcaaccg ccgcacctac cccaacctgt gtgcgctgtt cgcgctgctg
      481 caggtcgagg cggtggagac cgagatgagc ttcggcgtca gcctggccga acccgagctc
      541 gaatgggccg gcaccgacct tgccagcgtg ttcgcccagc gcggcaacct ggtgcgtcct
      601 gccttcctcg ccatgttgcg cgacatcctg cgcttcaacc gcgagaccac gcggatggcg
      661 agcgaaggtg gcatgccggc cctgtcgctc ggcgagtatc tcgacctgga ggactactcg
      721 caggccttcc gcgactggta cctgctgccc atggccgcgg cgatctggtc gtgtccgacg
      781 cgcaccatgc tcgcctatcc gctcgcgacc ttcgtgcagt tctgccacaa ccacggactg
      841 ctgcagatcc tcgatcgtcc gacctggatg acggtgaagg gcggcggacg cagctacgtg
      901 cgccgcatgc tcgcccgcct cgacgacgtg cgggtgaacg cgccggtgca gcgcgtggta
      961 cgcggcgagg acggggtgtg ggtgcacacc gcggccgggc cggagcgctt cgacgaggtc
     1021 gtcttcgcct gtcacagcga ccaggccctg gccatcctcg gcagcgaggc cacgccggac
     1081 gagcgccgca tcctcggcgc cgtgcgctac caggccaacg ttgcgtacct gcacaccgac
     1141 accgcgctcc tgccacgccg cccgaaggtg tggtcggcgt ggaactacct cgccggcaag
     1201 ggcgcgcccg acgcgcgtcc ggtgtcggtc agctacctca tcaaccgcct gcagccgctg
     1261 cctttccaga ccccggtcgt ggtgtcgctc aaccccttca tcgcgcccgc agccgacaag
     1321 accttccgtc gcatcgagta cgcccacccg gtgttcgacc acggcgcgat cgacgcccag
     1381 gccgcgctgc cttccatcca gggccgcgag cgctgctggt tcgccggcgc ctggaccggc
     1441 tacggctttc atgaagacgg cctcaagtcg gcggtggcgg tggtcgaggc gatgggcgtg
     1501 gaagtgccgt ggcggcgcgg ccgcgtgccg gcggcgaagc gggccgggac ggcgctggag
     1561 gcggcgtgat gcccgacgcg ctccagcccg cggtgtgctt cggcgcggtc atgcacgagc
     1621 gccacgtgca ggcgcacaac cgcttcgtgt acccgagcgc cttcctgcgc ctgccgctcg
     1681 gccgcctgga ccaagtgcgc gctccgctgc tcggcatcga gcgtgccaac ctgttcagct
     1741 tccgcagccg cgaccacggc gcgcgcgacg gctcgccctt gctgccgtgg atccgcgccc
     1801 tcctgaacga gcgcgggctg gccgcggcat gcgacggcga gatcgtgctg cagaccatgc
     1861 cgcgcattct cggcttcgtc ttcaacccgg tgagcttctg gttctgccac gaccgcgcgg
     1921 gcggcctgcg tgcggtgctg gccgaggtga acaacacctt cggcgagcgt cacaactacc
     1981 tgctgcacca cgccgacctg cgccccatcg gcgcgggcga tgagctgcgc gccagcaagc
     2041 gcttccatgt ctcccccttc ttcccggtgc gcggcgagta ccgcttccgc ttcgagcagc
     2101 gcggcgccgt gcatgcggtg tcgatcgagc tctgggacgg cggcgagcgc cagctctcca
     2161 cccgcctggc gggccgcgcc gaggccgtcg gtggggccag catggcgaag tggctcgcgc
     2221 gccagccctt catgaccctg ggcgtcgtcg cccgcatcca ctggcaggcg ctgcgcctgg
     2281 ccctgcggcg ggtgagcttc ttccgcaagc ccgcgccgcc gctcgaggag accacctgat
     2341 gaacgcactc gaccacgccc tgcagccgct cgccggcctg cgcctcgcgc gcctgccgcg
     2401 cgccacccgg ctgtgcctgg agatgctcga ccgcctcgac ggcggcgcga tcgcggtgga
     2461 gctgcccgac ggcgtgcgcg tgcgcgccgg ccacggtgcg ctcgtcgccc acctgcgcgt
     2521 gcgcgaccac gccgtgttcg acgaggcgct cgcacgcggc gacatcggct tcggcgaagc
     2581 ctggatggac ggcctgtggg acaccgagga cctccccggc ctgctgcgcc tgctctcggc
     2641 caaccgcagc cgcctgcagg gcgcgatcta cggccgcatg ttccgcctgc tcagccaccg
     2701 cctgcagcac ctgctgcgcg ccaacacgcg cagcggctcg cggcgcaaca tcgaggccca
     2761 ctacgacctc ggcaacgact tctatgcgct ctggctcgac cccaccatga cctactcggc
     2821 ggcggtgttc gcctcgcccg acgagtcgct gcaggacgcc cagctgcgca agtaccgccg
     2881 catcctcggc gagctcggcg cgcggcccgg gcagaccatc ctggaggtgg gctgcggctg
     2941 gggcggcttc gccgaggtcg ccgccaccga gttcggctgc aaggtgctgg gcatcacgct
     3001 gtcgccggcg cagctggcgt tcgcgcgtgc gcgcgccgag cgcggcggct tcgccgaccg
     3061 ggtggatctg gagctgtgcg actaccgcga cgtgcgcggc cagtacgacc atgtggtgtc
     3121 gatcgagatg atcgaggcgg tgggcgagcg cttctggccg acctacttcg cccagctctc
     3181 cgcactgctg aagccgggcg ggcgctgcgt ggtgcaggcg atcacgatcg ccgacgcgct
     3241 gttcgcgcgc taccggcgcg gcaccgactt catccagcgc cacgtcttcc ccggcggcat
     3301 gctgcccagc ccgggcgcgg tggcgcagca ggccgggcgc gccgggctcg aggtggtggg
     3361 cgacttcgcc ttcgggcgcg actatgcgcg cacgctcgcg cactggcatc ggagcttcga
     3421 cgcgcaggct gcagcggtgc gcgcccaggg cttccccgag cgcttcctgc gcatgtggcg
     3481 tttctatctc gcctactgcg aggcgggctt cgacaccggc gacctcgacg tgcatcacta
     3541 cgtgttcgcg catgccgccg cagggtcgca aggcggctga atggtgatgg gcgcagcgga
     3601 gggaagcgac ctgtgccgac cccctggccg cgccgtcccc gcattcgggg cctggaggtg
     3661 accccatgag cgccccgatc tgccttcccg cacgctcccc gcggcgcctg cagcgcagct
     3721 tcgagcggtt cgcccgcctc ccgcgcgcgc tcggcgcagt gctggcgtgc tgcatgctgc
     3781 tggcgctgga gacggtgcac gcgctcggcc tgccgccgca gctcgtggcg agcacggccg
     3841 ggctgcgcga ggtcggccgc ggcgagctgc gctggctcgg cttcaagctc tacgacgccg
     3901 cgctgtgggc gcgcgcgccg ggcgaacagg ccacggcaga cgagcacgtt ctgtcgatcc
     3961 gctacgcgcg cgcggtcacg tccgc
//
LOCUS       DAOQNN010000300         3979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14706_length_3979_cov_7.328236, whole genome shotgun sequence.
ACCESSION   DAOQNN010000300 DAOQNN010000000
VERSION     DAOQNN010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14706_length_3979_cov_7.328236"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(541..936)
                     /locus_tag="PKV97_13340"
     CDS             complement(541..936)
                     /locus_tag="PKV97_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06910.1"
                     /translation="MPRHPQKPHRPSSADQRSGERFGERMARAHRTRDELANNAWLRF
                     LCEVSTAWDSAKNEASLERIRAAVQAGARKRRLARLGLAMLQALLALFWEMLLAVSFP
                     LVALLIQVVVQLCIYAFMAYVIYKLLTVW"
     gene            1081..1365
                     /locus_tag="PKV97_13345"
     CDS             1081..1365
                     /locus_tag="PKV97_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNB06911.1"
                     /translation="MPKRDFYLAAYDVADPRRLVAALALVRGYVTGGQKSVHEVFLTA
                     GARHELLHDMALLLDETRDRFFLLRLDPSARTYALGVATKPADPDYFYVG"
     gene            1372..2355
                     /gene="cas1"
                     /locus_tag="PKV97_13350"
     CDS             1372..2355
                     /gene="cas1"
                     /locus_tag="PKV97_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNB06912.1"
                     /translation="MATLLLDRADLEVRFADGALALYENGTRSQTVPLRLLDRVVIQG
                     ARTRLDSTVLLKLAEAGAAILLQSPRMSRRVAIVLGPAHNDAAIRLAQTRQVLDAEFC
                     DAWSRKLVRSKLRHQLRLLDTAMAQRPDRRKPLFDARGGVADALATVVAEPAGSARLR
                     GLEGAAARAYFHGLTALFAPELAFTGRNRRPPRDPVNACLSLGYTLLHFEAVRSAHAA
                     GLDPLLGFYHRPAFGRESLACDLIEPLRPTVDAWVWRQFSTGQLRAEHFTDDRGACLL
                     GKTGRGHFYAGWERAARFPRRWLRLACARLARGMAREGKQWLEEAEDPDLF"
     gene            2372..3172
                     /locus_tag="PKV97_13355"
     CDS             2372..3172
                     /locus_tag="PKV97_13355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNB06913.1"
                     /translation="MVTPARRTHAAAALPPRKPLYLLASTPARMDAGPDHLVLRRDQG
                     APLRFPLARLCRIVCNRHVAWSGQALALCLEHGVPITWVDGRGHALGCTQGHQDEALP
                     FATLLETYLELPDWPARFANWRARRRLETLTTTAQRAARAGREFDPIAFSELKRELVY
                     NGTHPLVFPEQGQAWCEAIAIDRLQHQGLQACYWGFGASRLALGVELGALLWAELNLE
                     CGALPAGVAPGQSVARLFEAWAHEHEGRLLLHLGDLERHLAREVEAWQ"
     gene            3169..3516
                     /gene="cas2"
                     /locus_tag="PKV97_13360"
     CDS             3169..3516
                     /gene="cas2"
                     /locus_tag="PKV97_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNB06914.1"
                     /translation="MNAPRAWLISYDITDPRRLIRLHRFLVRQATPVQYSVFHFEGSP
                     AQMGRLMVEIETRIDPDTDDVRGYQLPARLQVDTLGRGSLPSGTALLSADAEALHALL
                     RPPPSMGGARSDG"
     gene            3554..>3979
                     /locus_tag="PKV97_13365"
     CDS             3554..>3979
                     /locus_tag="PKV97_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06915.1"
                     /translation="MLFSDWHSYDHYGLAFVAFFGTLAAVFLIQWVMMRSRWAGWMQS
                     LQGVAPPFMNALGVLFGLVLAFLANDTWSAHDRAMSAVYREADGLRGIAALADSLPGP
                     LRDEVRASLAAYARASADEWPQLARRGESTEVLARADAML"
BASE COUNT          594 a         1418 c         1329 g          638 t
ORIGIN      
        1 atcatgcgct ggggctcgtg gagcgggtgc gcgctgaaga caacacggat tcaggattga
       61 gacttgaggg cggcggcaaa gctttctcgg gcatagtgtt cgaactgatg ccctgaattc
      121 aaagggattg agactctgct gtgcgggata acagcgcaga ccttgtgttc gaactgatgc
      181 cctgaattca aagggattga gactccgttt cgctgacgat gtagcggctc gaaccgttcg
      241 aactgatgcc ctgaattcaa agggattgag acgattgcgt gccgttcagg taggcgacgg
      301 ccagatgttc gaactgacgc cttgaattca aagggatcga tagaagtcag cgcagcagtg
      361 ggtcgcggaa ggcgttcgtc cgaacgaacg cagtacccct ctcggccgcc tcgggcacgg
      421 cgcgaccttc tggagcagcg ctcgggaggc ctcctgcggg acgctgcgag gccggatgga
      481 tcgccctgcg caatggccac ggtctgctgc cgcgctgcgc tggcttggct gtcgcgcctg
      541 ctaccagacc gtcagcaact tgtagatgac gtacgccatg aaggcgtaga tgcacaactg
      601 cacgacgacc tggatcagca aggccaccag cggaaacgac accgcaagca gcatttccca
      661 aaacagcgcc agcagcgcct ggagcatggc cagccccagg cgcgcgaggc ggcgcttgcg
      721 ggcgcctgct tgcactgctg cgcggatgcg ctcgagagac gcctcgttct tggcgctgtc
      781 ccaggcagta ctcacctcgc acagaaagcg gagccacgca ttgttggcca actcgtcgcg
      841 agtccggtgg gcacgcgcca tccgctcacc gaagcgctcg ccggaacgct ggtcggcaga
      901 cgacggtcgg tgcggctttt gcgggtgacg gggcatgggg tttggccggc tgcttgaaca
      961 ggccgccagt gtcggcgtat cccggtcctt gccgcgcgat cgcaagcttg ccggcgctga
     1021 cgacggcccg gcagggcgcc atgatgggct cacccgcaaa ccaccgtgcc cgccgccatc
     1081 atgcccaagc gcgatttcta ccttgccgcc tacgacgtcg ccgatccgcg ccggctggtc
     1141 gccgcgctgg ccttggtgcg cggctacgtg accggcgggc agaagtcggt gcatgaggtc
     1201 ttcctcaccg cgggcgcgcg ccatgagctg ctgcacgaca tggcactgct gctcgacgag
     1261 acgcgcgacc gcttcttcct gctgcgcctc gacccgagcg cgcgcaccta tgcgctcggc
     1321 gtggcgacca agccggccga ccccgactac ttctacgtgg gatgaaccgc catggccacc
     1381 ttgctgctcg atcgtgccga cctcgaagtc cgtttcgccg atggcgcgct cgcgctgtac
     1441 gagaacggca cccgcagcca gaccgtgccg ctgcgcctgc tcgaccgggt ggtgatccag
     1501 ggcgcacgca cccggctcga cagcacggtg ctgctcaagc tcgccgaggc cggcgccgcc
     1561 attctgctgc agagcccgcg catgagccgg cgggtggcga tcgtgctcgg cccggcgcac
     1621 aacgacgccg cgatccggct tgcacaaacc cggcaagtgc tggatgcgga gttctgcgac
     1681 gcgtggtcgc gcaagctggt gcgcagcaag ttgcgccacc agctgcgcct gctcgacacc
     1741 gcgatggcgc agcggcccga ccgccgcaag ccgctgttcg acgcacgcgg cggcgttgcc
     1801 gacgcgctcg ccaccgtcgt cgccgagcct gccggtagtg ctcgcctgcg cgggctcgag
     1861 ggcgcagcgg cgcgtgcgta cttccacggc ctgacggcgc tgttcgcccc cgagctcgcc
     1921 tttaccggcc gcaaccggcg cccgccgcgc gacccggtga atgcctgtct gtcgctcggc
     1981 tataccctgt tgcatttcga ggcggtgcgc agcgcccacg ccgcggggct cgacccgctg
     2041 ctcggcttct atcaccgccc ggccttcggt cgcgagtcgc tcgcctgcga tctcatcgag
     2101 ccgctgcgtc cgacggtcga tgcctgggtg tggcggcaat tttccaccgg ccagctgcgc
     2161 gccgagcatt tcaccgacga ccgtggcgct tgtctgctcg gcaagaccgg ccgcgggcac
     2221 ttctacgccg gctgggaacg cgccgcccgc ttcccgcggc gctggttgcg actggcctgc
     2281 gccaggctcg cccgcggcat ggcgcgcgag ggcaagcaat ggctggaaga ggccgaggac
     2341 ccggacctgt tctgaacgcg gagacacgac catggttacg cccgcccgtc gtacgcatgc
     2401 cgccgccgcg ctgccgccgc gcaagccgct ctacctgctc gccagcacgc cggcacgcat
     2461 ggatgccggg ccggaccatc tggtgctgcg ccgcgaccag ggcgcgccgc tgcgttttcc
     2521 gcttgcccgc ctgtgccgca tcgtgtgcaa ccgccatgtc gcctggagcg gccaggcact
     2581 cgcgctctgc ctggagcacg gcgtaccgat cacctgggtg gacggccgcg gccacgccct
     2641 cggttgcacg caaggccatc aggacgaagc cttgcccttc gccacgctcc tggagaccta
     2701 tctggagttg ccggactggc ccgcgcgctt cgccaactgg cgcgcgcgcc gtcggctcga
     2761 gacgctgacc accaccgctc agcgcgccgc acgcgccggg cgggagttcg accccattgc
     2821 cttctccgag ctcaagcgcg agctcgtcta caacggcacc catccgctcg tcttccccga
     2881 gcaggggcag gcctggtgcg aggcgatcgc catcgaccgc ctgcagcacc aaggcctgca
     2941 ggcgtgctac tggggcttcg gggcgagtcg gctggcgctc ggggtcgagc tcggcgcact
     3001 gttgtgggcg gagctcaacc tcgaatgcgg cgcgctgccc gccggcgtcg cccccggtca
     3061 gagcgttgcc cgcctgttcg aagcctgggc gcacgagcac gaaggccgcc tgctgctcca
     3121 cctcggcgac ctcgagcgcc acctcgcccg cgaggtcgaa gcatggcaat gaacgcgccg
     3181 cgcgcctggc tgatcagcta cgacatcacc gacccacgta gactgatccg gctgcatcgc
     3241 ttcctggtcc gtcaggccac accggtgcag tactcggtgt tccacttcga gggcagcccg
     3301 gcgcagatgg gacggctgat ggtcgagatc gagacgcgta tcgaccccga taccgacgac
     3361 gtgcgcggct accagctccc cgcgcgcctg caggtcgaca ccctgggccg cggcagcctg
     3421 ccaagcggca ccgcgctgct gtctgccgac gccgaagccc tgcacgcgct gctgcgaccg
     3481 cccccgagca tgggcggggc gcgatcggac gggtagactg cgaggacctc ccgaacgccc
     3541 ctcctggatc tccatgcttt tctccgactg gcacagctac gaccactacg gcctcgcctt
     3601 cgtcgccttc ttcggcaccc tcgccgccgt gttcctgatc cagtgggtga tgatgcgctc
     3661 gcgctgggcg ggctggatgc agtccctgca gggggtggcg cccccgttca tgaacgcgct
     3721 cggcgtgctc ttcggcctgg tgctggcctt tctcgccaac gacacctgga gcgcgcacga
     3781 ccgcgcgatg agcgcggtgt atcgcgaggc ggacggcctg cgaggcatcg ccgccctggc
     3841 cgacagcctg ccgggaccgc tgcgcgacga ggtgcgcgcg tcgctggcgg cctacgcgcg
     3901 tgccagcgcg gacgaatggc cacaactcgc acggcgtgga gagagcaccg aggtgctggc
     3961 gcgcgccgat gccatgctc
//
LOCUS       DAOQNN010000301         3976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14726_length_3976_cov_10.771997, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000301 DAOQNN010000000
VERSION     DAOQNN010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14726_length_3976_cov_10.771997"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..801)
                     /gene="urtE"
                     /locus_tag="PKV97_13370"
     CDS             complement(109..801)
                     /gene="urtE"
                     /locus_tag="PKV97_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HNB06916.1"
                     /translation="MLKVDNLNQYYGGSHILRGLSFDVPVGKVTTLLGRNGVGKTTLL
                     KTLMGLVPAASGSISFHDQDITRAPSWKRVAAGIGFVPQGREIFPRLTVEENLLMGLA
                     TRPRGSAIPPRIFDMFPVLRQMMGRRGGDLSGGQQQQLAIGRALAFGPRLLILDEPTE
                     GIQPSIIKDIERAIRSLAETGEMAILLVEQYYDFAESLADQYLLMERGEFIMRGRGET
                     MPQDGVREALAV"
     gene            complement(808..1674)
                     /gene="urtD"
                     /locus_tag="PKV97_13375"
     CDS             complement(808..1674)
                     /gene="urtD"
                     /locus_tag="PKV97_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HNB06917.1"
                     /translation="MIPTLEIERERTRLDAERENEREHWDGSAADGRVFRPGELDLSH
                     KVILYLDDITVSFDGFRALNKLSLTIDAGELRCIIGPNGAGKTTMMDVITGKTRPDSG
                     TAFFGQTIDLTRLTEPEIAHAGIGRKFQKPTIFEQHTAFENLELAMKADKRVRRTLFA
                     SLIDDERDRVAAVLRQIRLDQAADRPAGLLSHGQKQWLEIGMLLMQEPRLLLLDEPVA
                     GMTDDETERTAELFTSLAGKHSLVVVEHDMAFVEALGGKVTVLCEGSVLAEGDLATVQ
                     ADPRVIEVYLGR"
     gene            complement(1671..2813)
                     /gene="urtC"
                     /locus_tag="PKV97_13380"
     CDS             complement(1671..2813)
                     /gene="urtC"
                     /locus_tag="PKV97_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HNB06918.1"
                     /translation="MRTPFILRLFHSMPRGGWIALAIFAAVAWIGVPVAHLALPESHP
                     LHVSAYTVSLLGKILCYMVVAVAMDLIWGYAGILSLGHGLFFALGGYAFGMYLMRQIG
                     RDGSYGAELPDFMVFLDWKELPWYWAGSDSLLWALFLVVALPGLVAFVFGWFAFRSRI
                     KGVYFSIITQALTYAAMLLFFRNETGFGGNNGFTDFKRILGHDITSPGMRVTLFALSA
                     ALLIACLVLGRYLVSSRFGRVLAAIRDAESRVMFIGYNPLHYKLFIWTLSAMLCGLAG
                     ALYVPQVGIINPSEMSPANSIEIAVWVAVGGRGTLVGAVLGAGIVNLAKSVFTVYLPE
                     YWPFVLGFLFIVVTLYLPDGVVGLWRKLRARRAAARLDDTAKGANA"
     gene            complement(2824..>3976)
                     /gene="urtB"
                     /locus_tag="PKV97_13385"
     CDS             complement(2824..>3976)
                     /gene="urtB"
                     /locus_tag="PKV97_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HNB06919.1"
                     /translation="DAAVREVLLYASAKLELASPDPALRLKAAQALGASSDAAVKNLL
                     AALLEKSGEGAAASWVEPDAEVRAAAAASMRAIDRRIGLAQTAGTLFSGLSLGSILLL
                     AALGLAITYGVMGVINMAHGELLMVGAYATFLVQGVFRAHFPEYLDFYVLAAIPAAFL
                     AAALVGIAMERLVLRHLYGRPLESLLATWGLSLVLIQAARVLFGPQNLELANPSWMSG
                     GVELAGGVVLPYNRIVILAFAAFVLVLIWLMMQKTRLGMFVRAVTQNRPMAGCVGVPT
                     ARVDTLAFAIGSGVAGLGGLALSQIANVGPAMGTGYIVDAFMVVVLGGVGQLAGAVWA
                     ALGLGIVAKFLEGWAGAVVAKILVLVFIIAFIQKRPQGIFALKGRFADS"
BASE COUNT          653 a         1316 c         1454 g          553 t
ORIGIN      
        1 aggcgaggtg cagctcggcg tgccaggccg gcgcgggctg gacggagggg aggcgcgagt
       61 tcatggccgg attatccgga taaggcggcg ggcgtgggag ctcgtcgctc agaccgccag
      121 cgcctcccgc acgccgtcct ggggcatggt ctctcccctg ccccgcatga tgaactcgcc
      181 gcgctccatc agcaggtact ggtcggccag gctctcggcg aagtcgtagt actgctcgac
      241 gagcaggatg gccatctcgc cggtctcggc gaggctgcgg atggcgcgct cgatgtcctt
      301 gatgatggag ggctggatgc cctcggtggg ctcgtcgagg atcagcagcc gggggccgaa
      361 ggcgagcgcg cggccgatcg cgagctgttg ctgctggccg ccggagaggt cgccgccgcg
      421 ccggcccatc atctgcctca gcaccgggaa catgtcgaag atgcgcggcg ggatggcgct
      481 gccgcgtggc cgggtggcca ggcccatgag caggttctcc tcgacggtga ggcgcgggaa
      541 gatctcgcgc ccctgcggca cgaagccgat gccggcggcg acgcgcttcc aggacggcgc
      601 gcgggtgatg tcctggtcgt ggaaggaaat gctgccgctg gcggcgggca ccaggcccat
      661 cagggtcttc agcagcgtgg tcttgcccac gccgttgcgc ccgagcagcg tcgtcacctt
      721 gccgacgggc acgtcgaagg acaggccgcg caggatgtgg ctgccgccgt agtactggtt
      781 caggttgtcg actttcagca tgttcgttca gcgccccagg tagacctcga tcacgcgcgg
      841 atccgcctgc accgtggcga gatccccctc ggccagcacc gaaccctcgc acagcaccgt
      901 gaccttgccg ccgagcgcct cgacgaaggc catgtcgtgc tcgaccacca ccagcgagtg
      961 cttgccggcg aggctcgtga acagctcggc ggtgcgctcg gtctcgtcgt cggtcatgcc
     1021 ggcgaccggc tcgtcgagca gcagcaggcg tggctcctgc atcagcagca tgccgatctc
     1081 cagccactgc ttctggccgt gcgagagcag cccggcgggg cggtcggcgg cctggtcgag
     1141 gcggatctgg cgcagcaccg cggcgacgcg gtcgcgctcg tcgtcgatga ggctggcgaa
     1201 cagggtgcgg cgcacgcgct tgtcggcctt catggccagc tcgaggttct cgaaggccgt
     1261 gtgctgctcg aagatggtcg gcttctggaa cttgcggccg atgccggcgt gggcgatctc
     1321 gggttcggtc aggcgggtga ggtcgatggt ctggccgaag aaggcggtgc ccgagtcggg
     1381 ccgcgtcttg ccggtgatca cgtccatcat ggtggtcttg ccggcgccgt tggggccgat
     1441 gatgcagcgc agctcgccgg cgtcgatggt caggctgagc ttgttgagcg cgcggaagcc
     1501 gtcgaagctc acggtgatgt cgtcgaggta gaggatgacc ttgtgcgaca ggtcgagctc
     1561 gccgggccgg aacacccgcc cgtccgcggc cgagccgtcc cagtgctcgc gctcgttctc
     1621 gcgctcggcg tcgaggcggg tgcgctcgcg ttcgatctcg agggtgggga tcatgcgttc
     1681 gctcccttgg cggtgtcgtc caggcgcgcg gcggcgcggc gcgcgcgcag cttgcgccac
     1741 aggccgacca cgccgtcggg caggtagagg gtgacgacga tgaacaggaa gccgagcacg
     1801 aagggccagt attccggcag gtagacggtg aacacgctct tggcgaggtt cacgatgccg
     1861 gcgccgagca ctgcgcccac cagcgtgccg cgcccgccca ccgccaccca cactgcgatc
     1921 tcgatcgagt tcgccggcga catctccgag gggttgatga tgccgacctg gggcacgtac
     1981 aaggcgcccg ccagcccgca cagcatcgcc gacagcgtcc agatgaagag cttgtagtgc
     2041 agcgggttgt agccgatgaa catgacccgg ctctcggcgt cgcggatcgc cgccagcacg
     2101 cggccgaagc gcgagctcac caggtagcgg ccgagcacca ggcaggcgat cagcagcgcc
     2161 gccgagagcg cgaacagcgt cacccgcatg ccgggcgagg tgatgtcgtg gccgaggatg
     2221 cgcttgaagt cggtgaagcc gttgttgccg ccgaagccgg tctcgttgcg gaagaacagc
     2281 agcatcgcgg cgtaggtcag cgcctgggtg atgatcgaaa agtacacccc cttgatgcgc
     2341 gagcggaagg cgaaccagcc gaacacgaag gccaccagcc cgggcagcgc gaccaccagg
     2401 aacagcgccc acagcaggga atccgagccc gcccagtacc agggcaattc cttccagtcg
     2461 aggaagacca tgaagtcggg cagctcggcg ccgtagctgc cgtcgcggcc gatctggcgc
     2521 atcaggtaca tgccgaaggc gtagccgccg agggcgaaga agaggccgtg gccgagcgac
     2581 aggatgccgg cgtagcccca gatcaggtcc atggcgacgg cgaccaccat gtagcacagg
     2641 atcttgccga gcaggctgac ggtgtaggcg gagacgtgca gcggatggct ctcgggcagc
     2701 gcgaggtgcg cgaccggcac gccgatccag gccacggcgg cgaagatcgc cagcgcgatc
     2761 cagccgccgc gcggcatgct gtggaacagt ctcaggatga agggcgtgcg catggcggcg
     2821 gcctcagctg tcggcgaagc ggcccttgag ggcgaagatg ccctgcggac gcttctggat
     2881 gaaggcgatg atgaagacca gcaccaggat cttggccacg accgcgcccg cccagccttc
     2941 gaggaacttg gcgacgatgc cgaggccgag cgccgcccat accgcgccgg cgagctggcc
     3001 gacgccgccg agcaccacca ccatgaaggc gtcgacgatg tagccggtgc ccatggccgg
     3061 gccgacgttg gcgatctgcg acagcgccag cccgcccagc ccggcgacgc cggagccgat
     3121 cgcgaaggcg agcgtgtcca cccgcgcggt gggcacgccc acgcagcccg ccatcggccg
     3181 gttctgggtg acggcgcgca cgaacatgcc cagccgcgtc ttctgcatca tcagccagat
     3241 cagcaccagc acgaaggcgg cgaaggcgag gatgacgatg cggttgtacg gcagcaccac
     3301 gccgccggcg agctcgacgc cgcccgacat ccatgacggg ttggcgagct ccaggttctg
     3361 cgggccgaag agcacgcgcg cggcctggat cagcaccagc gacaggcccc aggtggcgag
     3421 caggctctcc agcgggcggc cgtagaggtg gcgcagcacc aggcgctcca tggcgatgcc
     3481 gaccaaggcc gcagcgagga aggccgccgg gatcgcggcg agcacgtaga agtccagata
     3541 ttcggggaaa tgggcgcgga acacgccctg caccaggaag gtggcgtagg cgcccaccat
     3601 cagcagctcg ccgtgcgcca tgttgatgac gcccatcacg ccataggtga tcgccaggcc
     3661 gagtgcggcg agcagcagga tggagccgag cgacaggccg gagaacagcg tgccggcggt
     3721 ctgcgccagg ccgatgcggc gatcgatcgc gcgcatcgag gccgcggccg cggcgcgcac
     3781 ttcggcgtcc ggttcgaccc agctcgccgc tgcaccttcg ccgctcttct ccagcagcgc
     3841 ggcgagcagg ttcttgaccg cggcatcgga cgaggcgccg agcgcctgcg ccgccttcag
     3901 gcgcagggcg gggtcgggcg aggccagctc cagcttggcg ctggcgtaga gcagcacctc
     3961 gcgcaccgcg gcgtcc
//
LOCUS       DAOQNN010000302         3975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14731_length_3975_cov_8.765816, whole genome shotgun sequence.
ACCESSION   DAOQNN010000302 DAOQNN010000000
VERSION     DAOQNN010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14731_length_3975_cov_8.765816"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKV97_13390"
     CDS             complement(<1..411)
                     /locus_tag="PKV97_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HNB06920.1"
                     /translation="MAVTTVAQFAAELHRPAATLLEQLQHAGVNKTSASDALTDADKE
                     RLLAFLRNAHGTQSADRKKITLTKKSTSEIKQADASGKARTIQVEVRKKRVFVKREDL
                     PVDTAPADDEAERAEQARLEEQARLDAERQRLEEQ"
     gene            complement(497..1975)
                     /gene="nusA"
                     /locus_tag="PKV97_13395"
     CDS             complement(497..1975)
                     /gene="nusA"
                     /locus_tag="PKV97_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNB06921.1"
                     /translation="MNRELLMLVDAISREKNVDREVVFGAVESALASATKKLYEGEVD
                     IRVAIDRETGEYETFRRWHVVPDEAGLQLPEAEILHFEAKEQVDDIEVGDYLEEPVES
                     VPIGRIGAQAAKQVILQKIRDAEREMLLNDFLSRGDKIFSGTVKRLDKGDLIIESGRV
                     EGRLKRSELIAKENLRTGDRVRAMIMEVDPTLRGPQIVLSRSAPEFMIELFRQEVPEI
                     EQSLLEIKSCARDSGSRAKIAVVSHDKRVDPIGTCVGVRGSRVNAVTNELAGERVDIV
                     LWSEDPAQFVIGALAPANVQSIVVDEEKHAMDVVVDEENLAIAIGRGGQNVRLASELT
                     GWRINIMTAEESAAKQAQESDAIRKLFVEKLDVDEEVADILIAEGFTSLEEVAYVPLQ
                     EMLEIESFDEDTVHELRNRAKDVLLTMEIAQEERVDEVSQDLRDLEGLTPELIAKLAD
                     GGIHTRDDLADLAVDELTEMTGVDEAAAKALIMKAREHWFTA"
     gene            complement(1972..2574)
                     /gene="rimP"
                     /locus_tag="PKV97_13400"
     CDS             complement(1972..2574)
                     /gene="rimP"
                     /locus_tag="PKV97_13400"
                     /inference="COORDINATES: protein motif:HMM:NF000929.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNB06922.1"
                     /translation="MSWQLAVERTVAGMGYELVDFGRSAGGLLSVFIDRLPGRSYPTG
                     SGESITVDDCELVTRQLQYVLEVEGCDYARLEVSSPGLDRPLRKSADFVRFGGLEIDV
                     TLKRPFQGRKKYRGVLQPSADLAGEAALPESFELVFRDEQEDKVLGFALDEIREARLV
                     PVVDFKKGRKRRDAVEGADAADEAAQLPAGEAKESGGHEE"
     gene            complement(3071..>3975)
                     /locus_tag="PKV97_13405"
     CDS             complement(3071..>3975)
                     /locus_tag="PKV97_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB06923.1"
                     /translation="KMEGKLHERVVGQDEAIVAVSDAIRRSRAGLSDPNRPLGSFLFL
                     GPTGVGKTELCKALAGFLFDSEEHMIRIDMSEFMEKHSVSRLIGAPPGYVGYDEGGYL
                     TEAVRRKPYSVLLLDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIVMTSNL
                     GSHMIMQMAGQDSELIREAVWAEVKQHFRPEFLNRIDEAVVFHALDQSHIESIAKIQL
                     KALEARLAKMEMALEVTPEALAELAKVGFDPVFGARPLKRAIQQRIENPVAKLILEGR
                     FGPKDVIPVGVNTTGGFEFGRTVH"
BASE COUNT          646 a         1423 c         1193 g          713 t
ORIGIN      
        1 ctgctcctcg aggcgctggc gctcggcatc cagccgggcc tgctcttcca ggcgcgcctg
       61 ctcggcacgc tcggcctcgt cgtcggccgg agcggtgtcg accggcagat cctcgcgctt
      121 gacgaacacg cgcttcttgc ggacctcgac ctgaatggtg cgggccttgc cggaggcatc
      181 ggcctgcttg atctcgctgg tcgacttctt cgtcagcgtg atcttcttgc ggtcagcgct
      241 ctgggtgccg tgcgcattgc gcaggaaggc cagcaggcgc tccttgtccg cgtcggtgag
      301 cgcgtcgctg gccgacgtct tgttcacacc tgcgtgttga agttgctcga gcagcgtcgc
      361 agccgggcga tggagctcgg cagcgaactg ggcaacggtg gtcacagcca tgtcttgtat
      421 tccttgcgac gtgcgtgcga ggtgtcgctc tgggtcggta tccgtcgggt cgatctgggc
      481 ggcgtcggcg aagacgtcag gcggtgaacc agtgttcacg cgccttcatg atcagggcct
      541 tggcagcggc ctcgtcgacc ccggtcatct cggtcaactc gtccaccgcc agatcggcca
      601 gatcgtcgcg ggtgtggatg ccgccgtcgg ccagcttggc gatcagttcg ggcgtgaggc
      661 cctccagatc gcgcaggtcc tgcgacacct cgtcgacgcg ctcttcctga gcgatttcca
      721 tcgtcagcag cacgtccttg gcgcgattgc gcaattcgtg aaccgtatcc tcgtcgaagg
      781 attcgatctc cagcatttcc tgcagcggca cgtaggccac ctcctccagg ctggtgaacc
      841 cctcggcgat caggatgtcg gccacctcct cgtcgacgtc gagcttttcg acgaagagct
      901 tgcggatcgc atcgctctcc tgcgcctgct tggcggccga ctcctcggcc gtcatgatgt
      961 tgatcctcca gccggtcagc tcggaggcca ggcgcacgtt ctgcccaccg cggccgatgg
     1021 cgatcgcgag gttctcctcg tcgaccacca cgtccatggc gtgcttttct tcatcgacca
     1081 cgatggactg cacgttcgcc ggcgccagcg caccgatcac gaactgcgcc gggtcctccg
     1141 accacagtac gatgtccacc cgctcgccgg cgagctcatt ggtcacggcg ttcacccgcg
     1201 agccccgcac accgacgcag gtgccgatcg ggtcgacccg cttgtcgtgc gagaccaccg
     1261 cgatcttggc gcgcgaaccg gagtcgcggg cgcagctctt gatctccaac aggctctgct
     1321 cgatctcggg cacttcctgg cggaacagct cgatcatgaa ctccggcgcc gaacgcgaca
     1381 gcacgatctg cgggccacgc agcgtcgggt ccacctccat gatcatcgcc cgcacgcggt
     1441 cgccggtgcg caggttttcc ttggcgatca gctcgctgcg cttgagccga ccctcgacgc
     1501 gacccgactc gatgatcagg tcgcccttgt ccaggcgctt gacggtgccg gagaaaatct
     1561 tgtcaccccg cgacaggaag tcgttgagca gcatttcgcg ctcggcgtcg cggattttct
     1621 gcaggatcac ctgcttggcc gcctgcgcgc cgatgcgacc gatcggcacc gactcgaccg
     1681 gctcttccag atagtcgcca acctcgatgt cgtcgacctg ctccttggcc tcgaagtgca
     1741 ggatctcggc ctcgggcaat tgcagccccg cctcatcggg aacgacgtgc cagcggcgga
     1801 aggtctcgta ctcgccggtc tcacggtcga tggccacgcg gatgtccacc tcgccctcgt
     1861 agagcttctt ggtggcagag gccagcgccg actcgacggc accgaacacc acctcgcggt
     1921 cgacgttctt ctcgcgcgag atcgcatcca ccagcatcaa caattcgcga ttcattcttc
     1981 gtgacctccg gattcctttg cctcgcccgc aggcagttgc gccgcctcgt cagccgcgtc
     2041 ggccccctcg acggcatcac ggcgcttgcg ccccttcttg aaatccacca ccggcaccag
     2101 ccgtgcctcg cggatctcgt cgagcgcaaa acccaacacc ttgtcttcct gttcgtcgcg
     2161 aaacaccagc tcgaaggatt ccggcagcgc cgcctcgccc gccaggtccg ccgacggctg
     2221 cagcacaccc cggtacttct tgcgcccctg gaacgggcgc ttgagcgtga cgtcgatctc
     2281 gaggccgccg aaacgcacga aatcggccga cttgcgcagc ggacgatcca gccccggcga
     2341 ggacacttcg aggcgcgcgt aatcacagcc ctcgacctcg agcacatact gcaattggcg
     2401 cgtcaccagc tcacagtcgt cgacggtgat cgactcgccc gagccggtcg ggtagctgcg
     2461 ccccggcagc cggtcgatga aaacggacaa caagcccccc gcgctgcgac cgaagtcgac
     2521 gagctcatag cccatccccg ccacagtgcg ctccacagcc agctgccaac tcatcgactc
     2581 gcgttcccct cgaccagtgc aaaaggcagc ctgggcgacg cacccgacac gacgaatcgc
     2641 accgaacacg ccctcaggct gccgcagatc ctcccgcacc acccaccgaa accccgccaa
     2701 tggacggatt gatgtcgggt ggcaccgaag caaaaaaaat gggccggtca ataccgccca
     2761 tccagcgact taaacaaacc gagattatgc cccactttgc gacaggcaga caaggcagca
     2821 gcgtcggcgc cacagaaaac tcggcccgct cccgcaccac aggaatcgcg ccggcgactg
     2881 tgcagccggc cggaagagcc attcgctgtc cgcttcgacg cagcgatcaa ccccttccca
     2941 tcgaggcaaa caccggcccg accttccttg ttggcgttga attgtcagcc ctgcaatctg
     3001 ttctgagtct ccatgccgga agacgcggcg ccgacggggc cgagcgcccc cgcgcgccgg
     3061 gccgtccgcc tcagtgcacc gtgcggccga actcgaagcc gcccgtggtg ttcacgccca
     3121 ccgggatcac gtccttcggc ccgaaccggc cttccaggat cagcttggcc accgggttct
     3181 cgatgcgctg ctggatcgca cgcttgagcg gacgggcgcc gaacaccggg tcgaagccga
     3241 ccttggccag ctcggccagc gcctcgggcg tgacctccag cgccatctcc atcttcgcca
     3301 gccgggcctc cagcgccttg agctggatct tcgcgatcga ctcgatgtgc gactggtcga
     3361 gcgcgtggaa caccaccgcc tcgtcgatgc ggttcaggaa ctcggggcgg aagtgctgct
     3421 tcacctcggc ccagaccgcc tcgcggatca gctcactgtc ctgcccggcc atctgcatga
     3481 tcatgtgcga gccgaggttg ctggtcatca cgatcacggt gttcttgaag tccaccgtgc
     3541 ggccctggcc atcggtcagg cggccgtcgt cgagcacctg aagcagcacg ttgaacacgt
     3601 ccgggtgggc cttttccacc tcgtcgagca gcagcaccga gtagggcttg cggcgcaccg
     3661 cctcggtcag gtagccgccc tcgtcgtagc cgacgtagcc cggaggtgca ccgatcagcc
     3721 ggctcacgga gtgcttctcc atgaactcgc tcatgtcgat gcggatcatg tgctcctcgc
     3781 tgtcgaacag gaagccggcg agtgccttgc acagctcggt cttgcccacg ccggtggggc
     3841 cgaggaagag gaagctgccc agcggtcggt tggggtcgga aagaccggcg cggctgcggc
     3901 ggatggcgtc cgacaccgcg acgatggcct cgtcctggcc caccacgcgc tcgtgcagct
     3961 tgccctccat cttca
//
LOCUS       DAOQNN010000303         3942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14949_length_3942_cov_5.718549, whole genome shotgun sequence.
ACCESSION   DAOQNN010000303 DAOQNN010000000
VERSION     DAOQNN010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14949_length_3942_cov_5.718549"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="PKV97_13410"
     CDS             <1..1289
                     /locus_tag="PKV97_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNB06924.1"
                     /translation="SENRIGRATPAAVLAALARGALDPEPIAWQRLADYRAAGGYTLL
                     ERLRSGETGVAALEARLAEAGLRGLGGAGFPTARKWQAVRAGAAPRYLVVNADEGEPG
                     TFKDRHYLESAPHRVLEGALASALAVGAAAIYVYLRDEYPGLHAVLREAIAELEAAGL
                     VAPGFIVLRRGAGAYICGEESALIESLEGKPGKPRHRPPFVAEAGLFGRPTLVNNVET
                     LYWIPLLAASAAFAGEGRRRRSGLRSFSVSGRVNKPGVHLAPAGITLRELVDEHCGGL
                     QPGHRLLAYLPGGASGGILPAALADLPLDFDTLQPHGCFIGSAAIIVLSDQDDLRAVA
                     DNLLAFFADESCGQCTPCRLGTEKLLALLRTDDWDVARLQALAQTLRDASICGLGQAA
                     PNPVSSLLRFFPAELARAGVRLHAGPPARDAGELQP"
     gene            1286..>3942
                     /locus_tag="PKV97_13415"
     CDS             1286..>3942
                     /locus_tag="PKV97_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNB06925.1"
                     /translation="MSRHDREDLGSTPKPPNLATAPVVAAAHDALDQRADAFEIVLDG
                     VAVPAFPGETLWQVALRAGERIPHLCFKDAPGYRADGNCRACMVEIEGERVLAASCMR
                     AAQPGMVVRSASSARARQAREGVLELLLEQQPARTHSPDRSSHFWKLVEHIRLPAADR
                     GPAAQQPDRTHPAIHVNLDACITCGLCVRACREVQVNEVIGLAHRGARAKIVFDFDDA
                     LGDSSCVACGECVQACPTGALMPATLVDAQGRGDSAGAERRVDSVCPYCGVGCQLTYH
                     VRDERILFVEGRNGPANENRLCVKGRFGFDYPNHRGRLTTPLIRRAGVPKGVDPAFDP
                     ANPLSHFRPASWDEALDFAAAGLRRLLDAHGPGVLAGFGSAKCSNEEAWLLQKLVRTG
                     FGSNNVDHCTRLCHASSVAALMECIGSGAVTASFMQAAHADVVILTGCNPTVNHPVAA
                     TYFKQAAKRGTRLIVLDPRGLVLGHHAHRMVRFTPGSDVALFNAMLNVIVGEGLCDRD
                     FIAARTEGFEALAAHVAPLTPEAMAPLCGVAPDEIRAIARLYATAERAMIFWGMGISQ
                     HVHGTDNARCLIALALATGHVGRPGTGLHPLRGQNNVQGASDAGLIPMVLPDYRPVGD
                     AQYRAAFEELWATPLPAEPGLTVVETMDAIAAGRVRGMYILGENPAMSDPDLHHTRAA
                     LARLEHLVVQDLFVTETAQFADVILPASAWPEKDGTVTNTNRQIQLGRAAVPLPGEAR
                     PDWWILQQLARRLGLDWQYAHPREVFAEMKRAMRSLDHIDWARLEREGAVTTPCPAED
                     APGKDVVFDDRFPTASGRARFRPTVPLPPDEPVDAAWPTVLITGRQLEHWHTGAMTRR
                     SAVLDALEPAAVATLAPAELARLGLAPGAA"
BASE COUNT          519 a         1578 c         1331 g          514 t
ORIGIN      
        1 ccagcgagaa caggatcggc cgcgcgaccc cggccgcggt cctggccgca ctcgcccgcg
       61 gagcgctcga ccccgaaccg atcgcctggc agcgcctcgc cgactatcgc gccgcaggcg
      121 gctacaccct gctggagcgc ctgcggagcg gcgagaccgg cgttgccgcg ctcgaggccc
      181 ggctcgccga agccggcctg cgcggcctcg gcggtgccgg cttcccgacc gcgcgcaagt
      241 ggcaggcggt gcgcgccggc gccgcgccgc gctacctggt cgtcaatgcc gacgaaggcg
      301 agccgggcac cttcaaggac cgccactacc tggaatcggc accgcaccgg gtgctcgagg
      361 gcgcgctggc gagcgcgctc gcggtgggcg cggcggcgat ctacgtctac ctgcgcgacg
      421 aataccccgg cctgcacgcg gtgctgcgcg aggcgatcgc cgaactggag gccgccggcc
      481 tcgtcgcacc cggcttcatc gtgttgcgcc gcggcgccgg cgcctacatc tgcggcgagg
      541 agtcggcgct gatcgagtcg ctcgaaggca agccgggcaa gccgcgccat cgcccgccct
      601 tcgtcgccga ggccgggctc ttcggccgac cgacgctggt gaacaacgtc gagaccctgt
      661 actggatccc gctgctcgcc gccagcgccg ccttcgccgg cgaggggcgg cgccggcgca
      721 gcggcctgcg cagcttctcg gtgtctggcc gggtgaacaa gccgggcgtg cacctggccc
      781 ccgccggcat caccctgcgc gagctggtgg acgagcactg cggcggcctg cagcccggcc
      841 accgcctgct cgcctacctg cccggcggcg cctcgggcgg catcctgccc gccgcgctcg
      901 ccgacctgcc gctcgacttc gacaccctgc agccccatgg ctgcttcatc ggctcggcgg
      961 cgatcatcgt gctctcggac caggacgatc tgcgcgccgt cgccgacaac ctgctcgcct
     1021 tcttcgccga cgaatcctgc ggccaatgca cgccctgccg cctcggcacc gaaaagctgc
     1081 tcgcgctgct gcgcacggac gactgggacg tggcgcgcct gcaggccctc gcgcagaccc
     1141 tgcgcgacgc ctcgatctgc ggcctcggcc aggccgcgcc caacccggtg agcagcctgc
     1201 tgcgcttctt cccggccgag ctcgcgcgcg ccggggtgag gctgcatgcc gggcctcctg
     1261 cccgcgatgc cggggagctg cagccatgag ccggcacgat cgcgaggacc ttggctccac
     1321 gcccaagccc cccaacctgg cgacggcgcc agttgttgcg gcagcacacg acgcgctcga
     1381 ccagcgtgcg gacgccttcg agatcgtcct cgacggcgtc gcggtcccgg ccttccccgg
     1441 cgagacgctg tggcaggtcg cgctgcgcgc cggcgagcgc atcccccacc tgtgcttcaa
     1501 ggacgctccc ggctaccgcg ccgacggcaa ctgccgcgcc tgcatggtcg agatcgaggg
     1561 cgagcgcgtg ctcgccgcct cctgcatgcg cgcggcgcag ccgggcatgg tggtacgcag
     1621 cgccagctcg gcgcgggcac ggcaggcgcg cgagggcgtg ctcgaactgc tgctcgaaca
     1681 gcagccggca cgcacgcaca gccccgaccg ctccagccat ttctggaagc tggtcgagca
     1741 cattcgtttg cccgccgccg accgcggccc cgcagcgcag cagccggacc gcacccaccc
     1801 cgcgatccac gtgaacctgg acgcctgcat cacctgcggc ctgtgcgtgc gcgcctgccg
     1861 cgaggtgcag gtgaacgagg tcatcggcct cgcacaccgc ggtgcacgcg cgaagatcgt
     1921 cttcgacttc gacgatgcgc tcggcgacag cagctgcgtg gcctgcggcg agtgcgtgca
     1981 ggcctgtccc accggcgcgc tgatgcccgc caccctggtc gatgcgcagg gccgcggcga
     2041 ctcggccggc gccgagcgta gggtggattc ggtgtgcccc tactgcggcg tgggctgcca
     2101 gctcacctac cacgtccggg acgagcgcat cctcttcgtc gagggccgca acggcccggc
     2161 caacgagaac cgcctgtgcg tgaagggccg cttcggcttc gattacccca accaccgcgg
     2221 ccgcctgacg acgccgctga tccgccgcgc gggcgtgccc aagggcgtgg atcccgcctt
     2281 cgatccggcg aatccgctca gccacttccg cccggcgagc tgggacgagg cgctcgactt
     2341 cgccgccgcc ggcctgcgcc gcctgctcga cgcccacggc cccggcgtgc tcgccggctt
     2401 cggcagcgcc aagtgctcga acgaggaggc ctggctgctc cagaagctgg tgcgcacggg
     2461 cttcggctcc aacaacgtcg accactgcac ccggctgtgc cacgccagct cggtcgccgc
     2521 gctgatggag tgcatcggct cgggcgcggt cacggcctcc ttcatgcagg ccgcgcacgc
     2581 ggacgtggtc atcctcaccg gctgcaaccc gacggtgaac cacccggtcg ccgccacgta
     2641 cttcaagcag gccgccaagc gcggcaccag gctgatcgtg ctcgacccgc gcggcctggt
     2701 gctcggccac cacgcccacc gcatggtgcg cttcacgccg ggcagcgacg tcgcgctctt
     2761 caacgccatg ctcaacgtca tcgtcggcga gggcctgtgc gaccgcgact tcatcgccgc
     2821 gcgcaccgag ggcttcgagg cgctcgccgc ccacgtcgca ccgctcacgc ccgaggccat
     2881 ggcgccgctg tgcggggtgg cgccggacga gattcgcgcc atcgcccgcc tctacgccac
     2941 cgccgagcgc gcgatgatct tctggggcat gggcatctcg cagcatgtgc acggcaccga
     3001 caacgcgcgc tgcctgatcg cgctcgcgct cgccaccggc catgtcggcc gccccggcac
     3061 cggcctgcat ccgctgcgcg ggcagaacaa cgtgcagggg gcgtccgacg ccggcctcat
     3121 ccccatggtg ctgcccgact accgcccggt gggcgacgcg cagtaccgcg ccgccttcga
     3181 ggaactgtgg gcgacgccgc tccccgccga gcccggcctc acggtcgtcg agaccatgga
     3241 cgcgattgcc gccggccggg tgcgcggcat gtacatcctc ggcgagaacc cggcgatgtc
     3301 cgaccccgac ctgcaccaca cccgcgccgc gctcgccagg ctcgagcacc tcgtcgtgca
     3361 ggacctcttc gtcaccgaga ccgcgcagtt cgccgacgtg atcctgcccg cctcggcctg
     3421 gcccgagaag gacggcaccg tcaccaacac caaccgccag atccagctcg gccgcgccgc
     3481 cgtgccgctg cccggcgagg cgcggcccga ctggtggatc ctccagcagc tcgcccgccg
     3541 cctcggcctc gactggcagt acgcgcaccc gcgcgaagtc ttcgccgaga tgaagcgggc
     3601 gatgcgctcg ctcgaccaca tcgactgggc gcgtctggag cgcgagggcg cggtcacaac
     3661 cccttgcccg gccgaggacg ccccgggcaa ggacgtggtc ttcgacgacc gcttccccac
     3721 cgcgagcgga cgcgcgcgct tccgccccac cgtgccgctg ccgcccgacg agccggtcga
     3781 cgccgcctgg cccacggtgc tgatcaccgg ccgccagctc gaacactggc acaccggcgc
     3841 gatgacgcgg cgcagcgcgg tgctcgacgc gctcgaaccg gccgcggtgg cgacgctggc
     3901 cccggccgaa ctggcgcgcc tcggcctcgc tcccggtgcc gc
//
LOCUS       DAOQNN010000304         3941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_14955_length_3941_cov_9.821668, whole genome shotgun sequence.
ACCESSION   DAOQNN010000304 DAOQNN010000000
VERSION     DAOQNN010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14955_length_3941_cov_9.821668"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /gene="aroE"
                     /locus_tag="PKV97_13420"
     CDS             complement(<1..723)
                     /gene="aroE"
                     /locus_tag="PKV97_13420"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNB06926.1"
                     /translation="MDRYAVVGNPIAHSKSPWIHAAFARQTGQALTYEALLAPLDDFA
                     ATVARFRAEGGRGLNVTVPFKLEAFALADRRTPRAEAAGAVNTLAFGTDGILGDNTDG
                     AGLVRDLASHLGCPPRGRRILLLGAGGATRGALLPLLDTAPADLCIANRTAHKAVELA
                     QAFAAHAGTVRIEGCGFEALADQRFDLVINATSASLSGELPPLPPGLYAQGALAYDMM
                     YGRGHTPFLRAALADGAARVADG"
     gene            complement(734..1657)
                     /locus_tag="PKV97_13425"
     CDS             complement(734..1657)
                     /locus_tag="PKV97_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNB06927.1"
                     /translation="MTPPAAPVLPAGALPGAWSGRLRRFGGPLLVAAFVISISLHALL
                     LLIHFRLPEAKPARDKGLEVVLVNARHARAPDKAEVLAQANVDAGGNTEQEVRASTPL
                     PPQPNRQDGDALVEAKRRTPEPVREQKQVITRDKAPAKVAAAPKKAEQPTPTPEPQRE
                     ISGLDLLDSAAAVARIEAQIDRDLKELAKRPRKKFIGARAQEYRFAQYVEDWRQKVER
                     IGTLNYPDAARGRLYGSLLLSVTIRSDGTVERMGVHRSSGHKVLDDAALRIVKMAAPY
                     APFPPDIRKDYDVIEITRTWTFTNSDQVRAN"
     gene            complement(1665..3572)
                     /locus_tag="PKV97_13430"
     CDS             complement(1665..3572)
                     /locus_tag="PKV97_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNB06928.1"
                     /translation="MFVLYEEDGAFKAGTILADNDATLQVENTHGKRVKLKRAHVLME
                     FREPGPSDLLARAEAEAEGLDVEFLWEVCGDDEFAFADFAAEYHGHVPNAVESAALLL
                     RLHSAPIWFHRKGRGRFRKAPADILQAALAGLEKKRQQAAAIEHMRAELVAGRLPPEL
                     AALLPQALYRPDRNRPEIKALEAACVDSGLSAARLLLACGALASSYDFHYNRFLFEHF
                     PEGTAFPAIEPPVLPTALPRAEVAAFSIDDASTTEIDDAFSVTPRAEGGWRIGIHIAA
                     PALGFARGSTLDAVARRRLSTVYMPGNKITMLPDEIVQTFTLGEGRDCPAVSLYLDIT
                     PGLAIVHQESRVELVPIVANLRHHDIEPVFNDDTVHNGGPDFQWKRELTLLWDLATVL
                     EAGRGKAAGNEDRIDFGFSVDWTEETADGPGRVSIGRRLRGSPMDKLVAELMIHANMT
                     WGKLLDTSGIPGLYRAQGGGKVRMTTVAAPHEGLGVDCYAWSSSPLRRYVDLVNQWQI
                     ISVLQGTPPAFAPKSAELMAAMRDFELTYAEYAEFQRGMERYWCLRWLRQHGRGEVEA
                     TVLRENVVRLAEIPLVIRVPSMPLQMPGSRVKLVVERTDLLDVEVEARFVQTLAEPDP
                     ETVDEAGFGFA"
BASE COUNT          512 a         1303 c         1526 g          600 t
ORIGIN      
        1 cccgtcggcc acccgcgccg caccgtcggc gagcgcggcg cgcaggaagg gcgtgtggcc
       61 gcggccgtac atcatgtcgt aggcgagcgc gccctgggcg tagaggccgg gcggcagcgg
      121 cggcagttcg ccggacaggc tggcggaggt ggcgttgatc acgaggtcga agcgctggtc
      181 ggcgagcgcc tcgaagccgc agccctcgat gcgcaccgtg ccggcgtggg cggcgaaggc
      241 ctgcgccagc tccaccgcct tgtgcgccgt gcggttggcg atgcacaggt cagcgggtgc
      301 ggtgtcgagc agcggcaaca gcgcaccgcg ggtggcgccg ccggcgccca gcagcaggat
      361 gcgccgcccg cggggcgggc aaccgagatg gctcgcgagg tcgcgcacca ggccggcgcc
      421 gtcggtgttg tcgccgagga tgccgtccgt gccgaaggcg agcgtgttga ccgcaccggc
      481 cgcctcggca cgcggcgtgc ggcggtcggc gagggcgaag gcctcgagct tgaagggcac
      541 ggtgacgttg aggccgcgcc caccctcggc gcgaaagcgc gccaccgtcg cggcgaaatc
      601 gtcgagcggg gcgagcaggg cctcgtaggt gagcgcctgc ccggtctggc gagcgaacgc
      661 ggcgtggatc cagggcgact tgctgtgggc gatcgggttg ccgacgacgg cgtaacggtc
      721 catgggcggg gtctcagttg gcgcgcacct ggtcgctgtt ggtgaaggtc cacgtgcggg
      781 tgatctcgat cacgtcgtag tccttgcgga tgtcgggtgg gaagggggcg tagggcgcgg
      841 ccatcttgac gatgcgcagc gcggcgtcgt cgagcacctt gtggcccgag ctgcgatgca
      901 cgcccatccg ctccaccgtg ccgtcggagc ggatcgtcac cgacagcagc aggctgccgt
      961 agagccggcc gcgcgccgca tcggggtagt tcagcgtgcc gatgcgctcg accttctgcc
     1021 gccagtcctc gacgtactgc gcgaagcggt attcctgcgc gcgcgcgccg atgaacttct
     1081 tgcgcggacg cttggcgagt tccttgaggt cgcggtcgat ctgggcctcg atgcgggcga
     1141 ccgcggcggc gctgtcgagc aggtcgaggc cgctgatctc gcgctgcggc tcgggtgtcg
     1201 gcgtgggctg ctcggccttc ttgggcgccg cggcgacctt ggcaggggcc ttgtcgcgag
     1261 tgatcacctg cttctgttcg cgcaccggct cgggcgtgcg gcgcttggcc tcgaccaggg
     1321 cgtcgccgtc ctggcggttg ggctgcggtg gcagcggtgt gctcgcgcgc acctcctgct
     1381 cggtgttgcc gccggcatcg acgttggcct gcgcgagcac ctcggccttg tcgggtgccc
     1441 gggcgtggcg ggcgttgacc agcaccactt ccagaccctt gtcgcgcgcg ggcttggcct
     1501 cgggcaggcg gaagtggatc agcagcagca gcgcgtgcag gctgatcgag atgacgaagg
     1561 ccgccaccag cagcggtccg ccgaagcgcc gcaggcgccc gctccaggcc cccggcaggg
     1621 cgccggcggg caggacgggg gcggcgggcg gagtcatgcg cagatcacgc gaaaccgaag
     1681 ccggcctcgt ccaccgtctc gggatcgggt tcggcgagcg tctgtacgaa gcgcgcctcc
     1741 acctcgacgt cgagcaggtc ggtgcgctcg acgacgagct tgacccggct gccgggcatc
     1801 tgcagcggca tcgagggtac gcggatcacc agcggaatct cggcgaggcg caccacgttc
     1861 tcgcgcagca ccgtggcctc gacctcgccg cggccgtgct ggcgcagcca gcgcaggcac
     1921 cagtagcgct ccatgccgcg ctggaactcg gcgtactcgg cgtaggtgag ctcgaagtcg
     1981 cgcatcgccg ccatcagctc ggccgacttg ggcgcgaacg cgggcggggt gccctgcagc
     2041 accgagatga tctgccactg gttcaccaag tcgacgtagc gccgcagcgg cgagctcgac
     2101 cacgcgtagc agtccacccc gaggccttcg tgcggcgcgg cgaccgtggt catgcgcacc
     2161 ttgccgccgc cctgggcgcg atacaggccg gggatgcccg aggtatcgag cagcttgccc
     2221 caggtcatgt tggcgtggat catcagctcg gcgacgagct tgtccatcgg cgagccgcgc
     2281 aggcgccggc cgatgctgac gcgtcccggg ccgtccgcgg tctcctcggt ccagtccacg
     2341 ctgaagccga agtcgatgcg gtcctcgttg ccggcggcct tgccgcgtcc agcctcgagc
     2401 acggtggcga ggtcccacag cagggtcagt tcgcgcttcc actggaagtc ggggccgccg
     2461 ttgtggacgg tgtcgtcgtt gaacaccggc tcgatgtcgt ggtggcgcag gttggccacg
     2521 atcggcacga gctcgacccg gctctcctgg tggacgatcg ccagccccgg ggtgatgtcg
     2581 aggtagagcg acaccgccgg acagtcgcgg ccctcgccga gggtgaaggt ctgcacgatc
     2641 tcgtcgggca gcatggtgat cttgttgccc ggcatgtaga cggtggacag gcgccggcgc
     2701 gcgaccgcgt cgagcgtcga gccgcgcgcg aagccgagcg cgggtgcggc gatgtggatg
     2761 ccgatgcgcc agccgccctc ggcgcgcggc gtcaccgaaa aggcgtcgtc gatctcggtg
     2821 gtggaggcgt cgtcgatcga gaatgcggca acctcggcac gcggcagcgc ggtcggcagc
     2881 acggggggct cgatggcggg gaaggcggtg ccttcgggga agtgctcgaa gaggaagcgg
     2941 ttgtaatgga agtcgtagct cgaggcgagc gcaccgcagg ccagcagcag gcgcgcggcc
     3001 gacagcccgc tgtcgacgca ggcggcttcg agcgccttga tctctgggcg gttgcggtcg
     3061 gggcggtaga gcgcctgcgg cagcagtgcg gcgagctcgg gcggcaggcg cccggcgacc
     3121 agttcggcgc gcatgtgctc gatcgcggcg gcctgctggc gcttcttctc gaggccggcg
     3181 agcgcggcct ggaggatgtc ggcgggggcc ttgcggaagc gcccgcgtcc cttgcggtgg
     3241 aaccagatcg gcgccgagtg caggcgcagc agcagggcgg ccgattcgac cgcgttcggc
     3301 acatggccgt gatattcggc ggcgaagtcg gcgaaggcga actcgtcgtc gccgcacact
     3361 tcccagagga attcgacgtc caggccttcg gcctcggcct cggcgcgcgc gagcaggtcg
     3421 gaaggccccg gctcgcggaa ctccatcagc acatgggcgc gcttgagctt cacccgcttg
     3481 ccgtgggtgt tctcgacctg cagcgtggcg tcgttatcgg cgaggatggt tccggccttg
     3541 aaggccccgt cctcttcata cagcacgaac atcggcatcc gcctgaagaa agcaagcgcg
     3601 ctagtttact ggattcgggc cgccggccct atcgcggggt gcgaatgagg gggcgatgcc
     3661 gcgcgggggg cgatccggcg cggctccgat cggggctggc accgattcgg tgcggctcca
     3721 gtgcggtgcg gttcggcgtc ggcgtggttc ggagtcggtg cggcttcgat tcggccgcgg
     3781 ggccggggtg gcgggggccg cctgcactcg cggcccctcg ctgcgctcgg ggcttcccgc
     3841 ggtggaggac gccgcggggg cggcgacgca aactcgtcgc tgcgcgcctc ggacatgcgt
     3901 cgccttgttt cccccgcgac gccctccgcc tcggcgctcc t
//
LOCUS       DAOQNN010000305         3906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_15215_length_3906_cov_10.319657, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000305 DAOQNN010000000
VERSION     DAOQNN010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15215_length_3906_cov_10.319657"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PKV97_13435"
     CDS             <1..528
                     /locus_tag="PKV97_13435"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNB06929.1"
                     /translation="KIEFSLKDCLGRVWQCGTLQLDFNLPVRLGAEYVDEDNTKKIPV
                     MLHRAILGSLERFIGILIEHHAGAMPLWLAPVQAVVMNISEGQAEYAEEAVARLKKAG
                     FRVEADLRNEKINYKIREHSVQKLPYQIVIGEKEKAAGLVAVRARGGQDLGQMSLDSL
                     IERWLREVEAKAGSV"
     gene            555..1076
                     /gene="infC"
                     /locus_tag="PKV97_13440"
     CDS             555..1076
                     /gene="infC"
                     /locus_tag="PKV97_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNB06930.1"
                     /translation="MAQEKKQRVNREITAPEVRLVGEEGEQLGIVSLNASLSMAEEAG
                     LDLVEIAPMAVPPVCKLMDFGKFKYQEQKKAHEARLKQKQVQVKEVKLRPGTDENDYQ
                     IKLRNLKRFLEEGDKCKVTLRFRGREMAHQEFGLRQLERVKADLEELGQVEQMPKMEG
                     RQMIMIIAPKKKH"
     gene            1222..1419
                     /gene="rpmI"
                     /locus_tag="PKV97_13445"
     CDS             1222..1419
                     /gene="rpmI"
                     /locus_tag="PKV97_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNB06931.1"
                     /translation="MPKMKTKSGAKKRFKVRSSGGIKRSQAFKRHILTKKTTKVKRHL
                     RGMTEVHAADEKLIRAMLPYA"
     gene            1432..1791
                     /gene="rplT"
                     /locus_tag="PKV97_13450"
     CDS             1432..1791
                     /gene="rplT"
                     /locus_tag="PKV97_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNB06932.1"
                     /translation="MPRVKRGVTARARHKKVLSQAKGYRGRRKNVYRIAKQAVMKAGQ
                     YAYRDRRQRKRQFRTLWIARINAAARELGLTYSVFMNGLKKAAIEIDRKVLADLAVFD
                     KPAFAAIAEQARAKLAA"
     gene            1945..2976
                     /gene="pheS"
                     /locus_tag="PKV97_13455"
     CDS             1945..2976
                     /gene="pheS"
                     /locus_tag="PKV97_13455"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNB06933.1"
                     /translation="MEQLDQLVQQAAAEFAAAVDGAQLEQAKARYLGKSGALTERLKA
                     LGKLEPEARREAGAAINAAKTAIEAALESRRNALREAALLAQLAAEALDVTLPGRGGV
                     QGGLHPVSRTLERVERLFGSIGFVVADGPEIETDWHNFTALNTPENHPARSMHDTFYL
                     EGHDDVLLRTHTSPVQIRAMQAHVARHRDADAMPDLRIIAPGRVFRVDSDATHSPMFH
                     QVEGLWVGETVSFADLKGVIADFLRRFFETDDLQVRFRPSFFPFTEPSAEIDVAFMSG
                     ALEGRWLEIAGCGVVHPNVLRFGGIDPERYTGFAFGMGPDRLTMLRYGVNDLRLFFEG
                     DLRFLGQFR"
     gene            3026..>3906
                     /locus_tag="PKV97_13460"
     CDS             3026..>3906
                     /locus_tag="PKV97_13460"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNB06934.1"
                     /translation="MQFSEQWLRTFVDPQLDSAGLGHLLTMAGLEVEEAEPAAPAFRG
                     VVVARIVEAEKHPNADKLKLCKVDAGTGELLQIVCGAPNAAAGMKVPCAVVGAVLPGE
                     FEIKKAKLRGVESFGMLCSARELGFSEDHSGLYALPEDAPVGTDIRAYLGLDDTLFTI
                     KLTPNRADCLSLTGVAREVAAITGGEFRALAIEPVSPSIDARRDIVLDAPQACPRYCG
                     RILRGVDARAATPDWMARRLVRSGIRSISALVDITNYVMLELGQPLHAFDNTKLQGAI
                     HVRLPRAGEQVLLLNEQT"
BASE COUNT          729 a         1315 c         1260 g          602 t
ORIGIN      
        1 aagatcgagt tctcgctgaa ggattgcctg ggccgcgtgt ggcagtgcgg cacgctgcag
       61 ctcgacttca atctgccggt gcgcctcggt gccgagtatg tcgacgagga caacaccaag
      121 aagatcccgg tgatgctgca ccgtgccatc ctcggttcgc tggagcgctt catcggcatc
      181 ctgatcgagc atcacgccgg cgccatgccg ctgtggctcg ccccggtgca ggcggtggtg
      241 atgaacatct ccgagggcca ggccgaatat gccgaagagg cggtggcgag gctgaagaag
      301 gccggcttcc gcgtcgaggc ggatttgcgc aacgagaaga ttaactataa aattcgcgaa
      361 catagcgtgc agaagctgcc gtaccagatc gtcatcggcg agaaggagaa ggcggcaggc
      421 ctggtggccg tacgtgcccg gggtggccag gatcttggcc aaatgtccct cgattcgctg
      481 atcgaacgct ggcttcgcga agttgaagcg aaagccggat cggtctgata ttggttttcg
      541 tggagagttg aaccatcgca caagaaaaaa agcagcgcgt aaatcgggag atcactgcgc
      601 ctgaggtccg gctggtcggc gaggagggcg agcagctcgg tatcgtctcc ctgaacgcat
      661 ccctcagcat ggccgaagag gccgggctgg atctggtcga aatcgcaccg atggccgtgc
      721 cgcccgtgtg caagctgatg gatttcggca agttcaagta tcaggaacag aagaaggccc
      781 acgaagcccg actcaagcag aagcaggtgc aggtcaagga ggtcaagctc cgccccggca
      841 ccgacgagaa cgactaccag atcaagctgc gcaacctcaa gcgcttcctg gaagaaggcg
      901 acaagtgcaa ggtgaccttg cgcttccgcg gccgcgagat ggcgcaccag gagttcggcc
      961 tgcgccagct cgagcgcgtg aaggcggacc tcgaggaact cggccaggtc gagcagatgc
     1021 ccaagatgga agggcgccag atgatcatga tcatcgcgcc gaagaagaag cactgagctt
     1081 ctttctgaag aaattgcccc gcaaggggcg aggccgcggg aaggggtttc cctccggcgg
     1141 aaaacaagtg gtgccgggta acaaaacgcg ccgggctttt cggcgctacc tggcgtcatc
     1201 caaaaacatg gagtctcagc aatgcccaag atgaagacca agagcggagc gaagaagcgc
     1261 ttcaaggtcc gctccagcgg cgggatcaag cggtcccagg cgttcaagcg ccacatcctg
     1321 accaagaaga cgacgaaggt gaagcgtcac ctgcgcggca tgaccgaagt gcatgcggca
     1381 gatgaaaagc tgatccgcgc catgcttccg tacgcctgat aggagaccgc aatgccccga
     1441 gtcaaacgtg gtgtaaccgc ccgcgcccgt cacaagaaag tcctctccca ggccaagggt
     1501 taccgtggcc gtcgcaagaa cgtctatcgc atcgccaagc aggcggtgat gaaggcgggt
     1561 cagtacgcct accgcgaccg tcgtcagcgc aagcgtcagt tccgcaccct gtggatcgcg
     1621 cgtatcaacg ccgccgcgcg cgagctgggc ctgacctaca gcgtgttcat gaacggtctc
     1681 aagaaggccg cgatcgagat cgaccgcaag gtgctggccg atctggccgt gttcgacaag
     1741 ccggcgttcg ccgcgattgc cgagcaggcc cgggccaaac tcgctgcgta atcgcgcgca
     1801 gccaaaaaaa aggaggcctg gcctcctttt tttttctgga ttgccggcgc ccggcgggcg
     1861 ccgtgaccgg acccccgaat tcccgatgag cggcgcgccc cgcggcgctc atcccgcaac
     1921 accgcattcc gccagggcag ggacatggaa cagctcgatc aactggtaca acaggccgcc
     1981 gccgaattcg ccgccgcggt cgacggcgcg cagctcgaac aggccaaggc gcgctacctc
     2041 ggcaagtccg gcgcgctcac cgagcgcctg aaggcgctcg gcaagctcga gcccgaggcg
     2101 cgccgcgagg cgggcgccgc gatcaacgcc gccaagaccg cgatcgaggc cgcgctcgaa
     2161 agccgccgca acgcgctgcg cgaagccgcg ctgctcgccc agctcgccgc cgaggcgctc
     2221 gacgttaccc tgcccgggcg cggtggcgtg cagggcggac tgcatccggt gagccgcacc
     2281 ctcgagcgcg tcgagcgcct gttcggctcg atcggcttcg tggtcgccga cggtcccgag
     2341 atcgagaccg actggcacaa cttcaccgcg ctcaacacgc ccgagaacca cccggcgcgc
     2401 tcgatgcacg acaccttcta tctcgaaggc cacgacgacg tgctgctgcg cacccacacc
     2461 agcccggtgc agatccgcgc catgcaggcc cacgtcgcgc gccaccgtga cgccgatgcc
     2521 atgcccgacc tgcgcatcat cgcgcccggc cgtgtcttcc gcgtggactc cgatgccacc
     2581 cactcgccga tgttccacca ggtcgagggc ctgtgggtgg gcgagacggt gagcttcgcc
     2641 gacctcaagg gcgtgatcgc cgacttcctg cgccgcttct tcgagaccga cgacctgcag
     2701 gtgcgcttcc gcccgtcctt cttccccttc accgagcctt cggccgagat cgacgtcgcc
     2761 ttcatgagcg gcgcgctcga ggggcgctgg ctggagatcg ccggctgcgg cgtggtgcac
     2821 cccaacgtgc tgcgcttcgg cggcatcgac cccgagcgct acaccggctt cgccttcggc
     2881 atggggccgg accgcctcac catgctgcgc tacggcgtga acgacctgcg cctgttcttc
     2941 gagggcgacc tgcgcttcct cggccagttc cgctgagcgg cttcgcacaa gctgcgccct
     3001 accacgcaat ccgggtaatc gaatcatgca attctcagaa cagtggctgc ggaccttcgt
     3061 cgatccgcaa ctggacagcg ccggactcgg ccatctgctg accatggccg gcctcgaggt
     3121 cgaggaggcc gaacccgccg cgcccgcctt ccgcggcgtc gtggtggcca ggatcgtcga
     3181 ggccgagaag caccccaacg ccgacaagct caagctgtgc aaggtcgacg ccggcaccgg
     3241 cgaattgctg cagatcgtct gcggcgcgcc caacgctgcc gccggcatga aggtgccatg
     3301 cgcggtggtc ggcgcggtcc tgcccggcga gttcgagatc aagaaggcca agctgcgcgg
     3361 cgtcgagtcc ttcggcatgc tgtgctctgc gcgcgagctc gggttctccg aggatcactc
     3421 cggcctctac gcgctgcccg aggatgcccc ggtgggcacc gacatccgcg cctacctcgg
     3481 cctggacgac accttgttca ccatcaagct gacccccaac cgcgccgact gcctgagcct
     3541 gaccggcgtg gcgcgcgagg tcgcggcgat caccggcggc gagttccgtg cgctcgcgat
     3601 cgaaccggtt tcgccttcga tcgatgcgcg ccgcgacatc gtgctggatg cgccgcaagc
     3661 ctgtccgcgc tactgcggcc gcatcctgcg cggcgtggac gcgcgtgccg cgacccccga
     3721 ctggatggcg cgccgcctgg tgcgcagcgg catccgctcg atcagcgcgc tggtcgacat
     3781 caccaactac gtcatgctcg agctcggcca gccgctgcac gccttcgaca acacgaagct
     3841 gcagggcgcc atccacgtgc gcctgccgcg cgcgggcgag caggtgctgc tgctcaacga
     3901 gcagac
//
LOCUS       DAOQNN010000306         3877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_15398_length_3877_cov_8.514914, whole genome shotgun sequence.
ACCESSION   DAOQNN010000306 DAOQNN010000000
VERSION     DAOQNN010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15398_length_3877_cov_8.514914"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3877)
                     /locus_tag="PKV97_13465"
     CDS             complement(<1..>3877)
                     /locus_tag="PKV97_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leukotoxin LktA family filamentous adhesin"
                     /protein_id="HNB06935.1"
                     /translation="SADAKGGTVRIGSDHRSESSASATPAEDATASGDTLGIGASVAV
                     NVVANESRAELANGARLINPGAVTVEAVGDYLVHTEAEAGAEGGIAATPVAAVAVVDN
                     QTHAVIGSGERVQAGAKVGVTATHSSTVETSATGSAEGEKAAIGIAVAVNVLTERVSA
                     SIQRDVFTPGAIEVAAHNAHKGSATASASAKGGKGEEEDGSSAEDGVDLEVQRQSGFA
                     QDKQKDGSASKTQQPGSAESSEGKLSVAAAVAVNALDSRSEAYVASAIELQAGGRLAV
                     EAWSNTDAEAEADGSAAGSTATVGIGAAAAVNSIETHTLAWIGDAQVTANGIAVNAGT
                     EILGDDEENRFSASAKAGAGAGKVGIAGALALNLLTTHTRAEVLEGARVDAEGGDVEL
                     EAGSTTETSATALPDEPVDGGDFGVGASVALNLFREDEVRARIRRGAEITDLDKLRVI
                     ANASSDTRATAEAGAAGSVALDAAVALSDLKLNTAAVVEAGTEIVAAGAVEIRATSTG
                     EHEAEATGDTESEKVGIGASGAIVLSTTTTTASLQRSLQTTTSGEEGNLTIDADATRS
                     YEAVAKASAAGGKAEEDTTAAERDKASTSSTLKDNQDAQEGTQTTGNSNKLAVAAAVG
                     AVVMDDDVRADVGRAQGADSDPAVRLDVGGELAIGAHNQSDFSARGLGNTLDVSKLES
                     SAQVGIGVGVGLAIARNDTAATIGDGTLVADAAGVQVAAGSSQNTSAEFANKLAAEGV
                     AGAGAEKVGIAGALAVAWSQSTTSASLGEGVRIGDRTTGEYAGEVSITADNTSKLAAK
                     AWSAALAGKVGIGASIAILRANNSQHAWIGEQAEVTAAGLQLAGRNHLLNSSPSFDWT
                     ILDDLENRFTEANLQVLLGESNYYTETIAGAGGEQVAVTGSFSVNVFDELTEATIGDG
                     ARVNTNGTVDLEADNQSSATAFAGGVSAAGKVGVGLASADIVNRSQTRATIGADARIE
                     QSAAIDIDATAAMDLAAVTASAAAAGKAGVGGVLSLIDSENQVEAATGARSFLRSDGT
                     IDVDAGNTFDALGVAGVAGVGGTAGVGVSAGTFLVDNQTRASIGAAADVAAAGAIGVR
                     GTAVQDVTSVVVGGAGGGTAGVAASAAVNDYNPLTYAWIGDGARINQGVSGAALAGRS
                     LAVHAHARTDLLSIVGTVGIGGTAGVGGAADVVVIDKETAAWIGEGVIARSGDSLAVG
                     ASSAEAIRSVGVGLSAGGTAGVQGSASVLVLDIDTLASVKTGSALFSDGNLVIAADAD
                     SDLDLLAGAIGAAGTAAVGAGAAVAVVDKT"
BASE COUNT          394 a         1419 c         1415 g          649 t
ORIGIN      
        1 ggtcttgtcc accaccgcga ccgccgcgcc ggcgcccacc gccgcggtcc ccgccgcgcc
       61 gatcgcgccg gcgagcaggt cgaggtcgct gtcggcgtcg gccgcgatca ccaggttgcc
      121 gtcggagaac agcgcgctgc ccgtctttac cgaggcgagc gtgtcgatgt cgagcaccag
      181 caccgaagcc gagccctgca caccggccgt gccgcccgcg ctcaggccca cgcccaccga
      241 gcggatcgcc tctgcgctgc tggcgcccac cgcgaggctg tcgccgctgc gggcgatcac
      301 gccctcgccg atccaggccg cggtctcctt gtcgatcacc accacgtcgg cggcgccgcc
      361 gacgccggcg gtgcccccga tgcctaccgt gccgacgatg gacagcaagt cggtgcgggc
      421 gtgcgcgtgc accgccaggc tgcggcccgc aagggccgcg ccgctcacgc cctgattgat
      481 gcgtgcgccg tcgccgatcc acgcgtaggt gagcgggttg tagtcgttca ccgccgcgga
      541 tgcggctacg cccgcggtgc cgccgccagc gccgccgacg acgaccgagg tgacatcctg
      601 caccgcggtg ccgcgcacgc cgatcgcgcc cgccgccgcg acgtcggccg cggcgccgat
      661 cgaggcgcga gtctggttgt cgaccaggaa ggtgccggcg gatacgccca ccccggcggt
      721 tccgcccacg ccggccactc cggcgacgcc gagggcatcg aaggtgttgc cggcgtcgac
      781 gtcgatggtg ccgtcgctgc gcaggaagct gcgtgcgccg gtggcggcct cgacctggtt
      841 ctccgagtcg atcaggctca gcacgccgcc cacgccggcc ttgcccgcgg cggccgcgct
      901 cgcggtgacc gcggcgagat ccatcgcggc cgtggcgtcg atgtcgatcg cggcggactg
      961 ctcgatgcgc gcgtccgcgc cgatcgtggc ccgggtctgg ctgcggttga cgatgtcggc
     1021 gctggccagg cccacgccga ccttgcccgc ggccgacacg ccgccggcga aggcggtcgc
     1081 ggaggactgg ttgtcggctt ccaggtcgac cgtgccgttg gtgttcaccc gcgcgccgtc
     1141 gccgatcgtg gcctcggtga gttcgtcgaa gacattcacc gagaacgagc cggtgaccgc
     1201 gacctgctcg ccgccggcgc cggcgatggt ctcggtgtag tagttgcttt cgccgagcag
     1261 cacctgcagg ttggcctcgg tgaagcggtt ctcgaggtcg tcgaggatcg tccagtcgaa
     1321 gctcggcgag ctgttcagca gatggttgcg ccccgcgagc tgcagcccgg ccgcggtgac
     1381 ttcggcctgt tcgccgatcc aggcatgctg gctgttgttg gcgcgcagga tggcgatcga
     1441 ggcgccgatg ccgaccttgc cggcgagcgc cgcgctccag gccttggccg cgagcttgct
     1501 ggtgttgtcc gcggtgatgc tgacttcgcc ggcgtactcc ccggtggtcc gatcgccgat
     1561 gcggacgccc tcgcccagcg aggcggaggt ggtggattgc gaccatgcca ccgcaagtgc
     1621 gcccgcgatg ccgaccttct ccgccccggc gcccgccacg ccttctgcgg cgagcttgtt
     1681 ggcgaactcc gccgaggtgt tctgcgacga tccggcggca acttgcacgc ccgccgcgtc
     1741 cgcgaccagc gtgccgtcgc cgatcgtggc ggcggtgtcg ttgcgcgcga tcgccaaccc
     1801 gacaccgaca ccgatgccga cctgggcgct gctctcgagc ttggacacgt ccagggtgtt
     1861 gcccaggccg cgggcgctga agtcgctctg gttgtgtgcg ccgatcgcga gttcaccgcc
     1921 gacgtcgagg cggacggcag ggtcgctgtc cgccccctgc gcacgcccga cgtccgcgcg
     1981 cacgtcgtcg tccatgacca ccgcgcccac cgcggccgcc accgccagtt tgttgctgtt
     2041 gccggtggtc tgggtgccct cctgggcgtc ctggttgtcc ttcagggtcg agctggtgct
     2101 ggccttgtcg cgctcggcgg cggtggtgtc ctcctcggcc ttgccgccgg cggcgctggc
     2161 cttggccacc gcctcgtagc tgcgcgtggc gtcggcgtcg atggtgagat tgccctcctc
     2221 gccgctggtg gtcgtctgca gcgagcgctg caggctggcg gtggtcgtgg tcgtgctgag
     2281 cacgatcgcg cccgaggcgc cgatgccgac cttttccgat tcggtgtcgc ccgtcgcctc
     2341 ggcctcgtgc tcgccggtgg aggtggcgcg gatctcgacg gcgccggcgg cgacgatttc
     2401 ggtgccggcc tcgaccacgg cggccgtgtt cagcttgagg tcggacaggg ccacggcggc
     2461 gtcgagcgcc acgctgcccg cagcgcctgc ctcggcggtg gcgcgggtgt cgctgctggc
     2521 gttggcgatc acacgcagct tgtcgaggtc ggtgatctcg gcgccgcgcc ggatgcgtgc
     2581 ccgcacctcg tcctcgcgga agaggttgag cgcgaccgag gcaccgacgc cgaagtcgcc
     2641 gccgtcgacc ggctcgtcgg gcagggcggt ggcgctggtt tccgtggtgc tgccggcttc
     2701 gagctcgaca tcgccgccct cggcgtccac gcgcgcgcct tcgagcacct cggcgcgggt
     2761 gtgggtcgtg agcaggttga gcgcgagcgc gccggcgatg ccgaccttgc ccgcgccggc
     2821 gccagccttc gcgctcgcgc tgaagcggtt ctcctcgtcg tcgccgagga tttccgtgcc
     2881 ggcgtttacg gcgatgccgt tcgcggtcac ctgagcgtcg ccgatccagg ccagggtgtg
     2941 ggtctcgatg ctgttgaccg cggcggcggc gccgatgccg accgtcgccg tgctcccggc
     3001 cgcgctgcca tcggcctcgg cttcggcgtc ggtgttgctc caggcctcga ccgcgagccg
     3061 gccgccggcc tgcaactcga tggcgctggc gacgtaggcc tcgctgcggc tgtcgagcgc
     3121 gttcaccgcg accgccgcgg ccacgctgag cttgccctcg ctcgattcgg ccgagcccgg
     3181 ctgctgggtc ttgctcgcgc tgccgtcctt ctgcttgtcc tgggcgaagc cggactggcg
     3241 ctggacctcg aggtccacgc cgtcctccgc gctgctgccg tcctcttcct cgcccttgcc
     3301 gcccttggcg ctcgcgctcg cagtggccga gcccttgtgg gcgttgtgcg cggccacttc
     3361 gatcgcgccc ggggtgaaca cgtcgcgctg gatgcttgcg ctgacgcgtt cggtgagcac
     3421 attgaccgcc acggcgatgc cgatcgcggc cttctcgccc tcggcgctgc ccgtggccga
     3481 ggtttcgacg gtgctggaat gggtggcggt gacgccgacc ttggcgccgg cctgcacgcg
     3541 ttcgccgctg ccgatcacgg catgggtctg gttgtcgacc accgcgaccg cggccaccgg
     3601 ggtggcggcg atgccgccct cggcgccagc ctcggcctcg gtgtggacga ggtagtcgcc
     3661 gacggcctcg accgtgacgg cgcccgggtt gatcaggcgc gcgccgttgg cgagctcggc
     3721 gcggctctcg ttggcgacga cgttgaccgc caccgaggcg ccgatgccca gggtgtcgcc
     3781 gctggcagtg gcgtcctcgg cgggggtggc gctggccgag gactcgctgc ggtggtcgct
     3841 gccgatgcgg acggtgccgc ccttcgcgtc ggcgctg
//
LOCUS       DAOQNN010000307         3866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_15472_length_3866_cov_11.323012, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000307 DAOQNN010000000
VERSION     DAOQNN010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15472_length_3866_cov_11.323012"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /gene="mrdA"
                     /locus_tag="PKV97_13470"
     CDS             complement(<1..1930)
                     /gene="mrdA"
                     /locus_tag="PKV97_13470"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNB06936.1"
                     /translation="MTEFRAPAVELARFRLRVVVAAVFVLVCFGLLAARFHHLQVKRH
                     DYFLTRAEDNRIALLPVVPHRGTIVDRKGVVLARNYATYTLEITPSQVRDLDATLDEL
                     ASLLPIDGRDRRRFRKLYEESRNFESVPLRSRLTDEEVARVVSQRYRLPGVDVKARLL
                     RDYPQGTTASHVIGYIGRITERDVERIEEAGNAANYRGSQHMGKAGLEQSYEHELHGT
                     TGFEQVEVNAGGRAVRALSHTPATPGNDLELTLDIELQKVAEKAFGDRRGALVAIEPS
                     TGGVLALASMPTYDPNLFVDGISTQDWKALNDSPDHPMLHRAIYSTYPPGSTFKPFMA
                     LAGLETGKRTARQAIHDVGYFNSGGHRFMDDKIGGHGMVDLHKSIVVSCNTYYYQLAN
                     DLGIEGIAGFMAPFGFGERTGIDLPGEATGVLPSPEWKRKRFKKAEQQRWFGGETISV
                     GIGQGYNAYTPLQLANALAALVNRGRLYRPHVVKYVVDSRTGERRAVEPEPLRQIPLR
                     DAHLRAVLDAMVDVNRTGTGKRAFKGAPYTVGGKTGTAQVFSLRGQRYVEGRVRERLR
                     DHSWFVAYAPAENPKIALAVLVENGGFGAQSAAPIARQVIDYYLLDQRAGAPAAEDEE
                     GAESEEGAEGGGEASPATP"
     gene            complement(1943..2464)
                     /gene="mreD"
                     /locus_tag="PKV97_13475"
     CDS             complement(1943..2464)
                     /gene="mreD"
                     /locus_tag="PKV97_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNB06937.1"
                     /translation="MHPTNRSSRILQPVKLWFVYLSLFVALGLEYIPTGRTPGLPDWV
                     ALALAFWCVREPLAIGMGAGFAFGLLMDVGLGAAMGQHALAYVVLAYLANALARRVLW
                     FPAWQQALHVLPLLLLAQAIMALVRLVAGAEFPGWAYFLSSFGSALLWTPLSFLLLLP
                     QYQPDERDDNRPI"
     gene            complement(2468..3346)
                     /gene="mreC"
                     /locus_tag="PKV97_13480"
     CDS             complement(2468..3346)
                     /gene="mreC"
                     /locus_tag="PKV97_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNB06938.1"
                     /translation="MSLVGHQPPPIFRRGPAPLARLFVFVALCLVLLVADLRFRYLEV
                     LRNALSVVTYPLQMAASTPADVVRNASRYFGTLVDVQLENAELRRHQLGAGERLLRFE
                     QLEQENLHLRELLQMSQRVQTKSIAADILYNAPDPFARKVILDRGTQQGVEAGLAVLD
                     ANGVIGQVTRVHPVQSEVTLLTDRNQSIPVSVVRNGVRGVLYGVGRGMLEMRHVLAEV
                     DIQPGDRLVTSGLDGIFVPGLPVATVTRVDRDQDAFARIECEPLAAIERSVQVLVIGR
                     AAYPPPPPPPEPAGGR"
     gene            complement(3418..>3866)
                     /locus_tag="PKV97_13485"
     CDS             complement(3418..>3866)
                     /locus_tag="PKV97_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNB06939.1"
                     /translation="VNYIRRNYGMLIGETTAEKIKKQIGSAFPGSEVREMEVKGRNLA
                     EGIPRSFTISSNEILEALTEPLNQIVSAVKIGLEQTPPELGADIAERGMMLTGGGALV
                     RDLDRLLMEETGLPVVVAEEPLTCVARGCGMALDKMDKLASIFTSE"
BASE COUNT          598 a         1295 c         1412 g          561 t
ORIGIN      
        1 ccggcgtcgc cggcgacgcc tcgccgccgc cctccgcgcc ctcctcgctc tcggcgccct
       61 cctcgtcctc ggcggccggc gcgccggcac gctggtcgag caggtagtag tcgatcacct
      121 ggcgcgcgat cggcgccgcc gactgggcgc cgaagccgcc gttctcgacc agcaccgcga
      181 gcgcgatctt cggattctcg gccggggcgt aggcgacgaa ccaggagtgg tcgcgcaggc
      241 gctcgcgcac gcggccctcg acgtagcgct ggccgcgcag cgagaacacc tgggcggtgc
      301 cggtcttgcc gccgaccgtg tagggcgccc ccttgaacgc gcgcttgccg gtgcccgtgc
      361 ggttgacgtc gaccatcgcg tcgagcaccg ccctcaggtg cgcatcccgc agcggaatct
      421 ggcgcagggg ctcgggttcg accgcacgcc gctcgccggt gcgcgaatcg accacgtact
      481 tgaccacgtg cgggcggtac agacggcccc ggttgaccag cgcggcgagc gcgttggcga
      541 gttgcagcgg cgtgtaggcg ttgtagccct ggccgatgcc caccgagatc gtctcgccgc
      601 cgaaccagcg ctgctgctcg gccttcttga agcgcttgcg cttccactcc ggcgagggca
      661 gcacgccggt ggcctccccg ggcaggtcga tgccggtgcg ctcgccgaag ccgaaggggg
      721 ccatgaagcc ggcgatgccc tcgatgccga ggtcgttggc gagctggtag taataggtgt
      781 tgcacgacac cacgatcgac ttgtgcaggt ccaccatgcc gtggccgccg atcttgtcgt
      841 ccatgaaacg gtggccgccg gagttgaagt agccgacgtc gtggatcgcc tgccgcgcgg
      901 tgcgcttgcc ggtctcgagg ccggcgagcg ccatgaaggg tttgaaggtg gagccgggcg
      961 gataggtgga atagatcgcg cggtgcagca tcgggtggtc gggcgaatcg ttgagcgcct
     1021 tccagtcctg ggtcgagatg ccgtcgacga agaggttggg gtcgtaggtc ggcatcgacg
     1081 ccagcgcgag cacgccgccg gtcgagggct cgatcgccac cagggcgccg cggcgatcgc
     1141 cgaaggcctt ctcggccacc ttctgcagtt cgatgtcgag ggtcagctcg agatcgttgc
     1201 ccggcgtcgc cggagtgtgc gacagcgcgc gcaccgcgcg cccgccggcg ttgacctcga
     1261 cctgctcgaa gccggtggtg ccgtgcaact cgtgctcgta ggactgctcc aggccggcct
     1321 tgcccatgtg ctggctgccg cggtagttgg ccgcgttgcc ggcctcctcg atgcgctcga
     1381 cgtcgcgctc ggtgatgcgg ccgatgtagc cgatcacatg cgatgcggta gttccctgcg
     1441 ggtagtcgcg cagcaggcgg gccttcacgt cgacgccggg caggcggtag cgctgcgaga
     1501 ccacgcgtgc gacctcctcg tcggtgagcc ggctgcgcag cggcacgctc tcgaagttgc
     1561 ggctctcctc gtagagcttg cggaaacggc ggcggtcgcg tccgtcgatc ggcagcaggc
     1621 tggcgagctc gtcgagcgtg gcgtcgaggt cgcgcacctg cgagggggtg atctcgagcg
     1681 tgtaggtggc gtagttgcgc gccagcacca cgcccttgcg atcgacgatg gtgccgcggt
     1741 gcggcaccac cggcagcaac gcgatgcggt tgtcctcggc gcgggtgagg aagtagtcgt
     1801 ggcgcttgac ctgcaggtgg tggaagcgcg ccgcgagcag accgaaacag acgagcacga
     1861 acaccgccgc caccacgacg cgaagccgga agcgggccag ttcgaccgcg ggcgcacgaa
     1921 actcggtcat cgcccgcccg cctcagatcg ggcgattgtc atcgcgctcg tccggctggt
     1981 actgcggcag cagcagcagg aaggacagcg gcgtccacag cagcgcgctg ccgaagctcg
     2041 acaggaaata cgcccacccg gggaactccg cccccgccac caggcgcacc agcgccatga
     2101 tcgcctgcgc cagcagcagc aggggcaaca cgtgcagcgc ctgctgccag gccgggaacc
     2161 acagcacgcg gcgggcgagc gcgttggcca gataggccag cacgacatag gcgagcgcgt
     2221 gctgccccat cgccgcgccg aggccgacgt ccatcagcag gccgaaggcg aaacccgcgc
     2281 ccatgccgat cgcgagcggc tcgcgcacgc accagaaggc caacgccagc gccacccagt
     2341 cgggcaggcc gggcgtgcgt ccggtgggga tgtactccag gccgagcgcg acgaacaggc
     2401 tcaggtagac gaaccacagc ttgaccggct gcaggatgcg gctggaacgg ttggtggggt
     2461 gcatggttca gcgccctccc gcgggttcgg gcggcggcgg aggcggcgga tacgcggcgc
     2521 gcccgatcac cagcacctgc acgctgcgct cgatcgccgc cagcggctcg cattcgatgc
     2581 gcgcgaaggc gtcctggtcg cgatcgaccc gggtcaccgt cgccaccggc aggccgggca
     2641 cgaagatgcc gtcgaggccc gaggtgacga ggcggtcgcc aggctggata tcgacctcgg
     2701 cgagcacgtg gcgcatctcc agcatgccgc ggccgacgcc atagagcacg ccgcgcacgc
     2761 cgttgcgcac cacgctgacc ggaatggact ggttgcggtc ggtgagcagg gtgacttcgg
     2821 actggaccgg atggacgcgg gtgacctggc cgatcacgcc gttggcgtcg agcaccgcga
     2881 gaccggcctc gacaccctgc tgggtgccac ggtcgaggat caccttgcgc gcgaaggggt
     2941 cgggcgcgtt gtagaggatg tcggcggcga tgctcttcgt ctgcacgcgc tgcgacatct
     3001 gcagcagctc gcgcagatgc aggttctcct gctcgagctg ctcgaagcgc agcaggcgtt
     3061 cgccggcgcc gagctggtgg cggcgcaact cggcgttctc gagctgcaca tcgaccaggg
     3121 tgccgaaata gcgcgaggcg ttgcgcacca cgtcggccgg cgtcgacgcc gccatctgca
     3181 gcgggtaggt gaccactgac agcgcgttgc gcagcacctc gagatagcgg aagcgcaggt
     3241 cggccacgag cagcacgagg cacagcgcga cgaacacgaa caggcgcgcc aagggtgccg
     3301 ggccgcgccg gaagatggga gggggctgat ggccgacgag agacatccgc tacgaaccag
     3361 ggccgtcggg tggagaggag aaacgcgccc cggcgcgcgc accggggcag gagagcgtca
     3421 ctcggacgtg aagatggagg cgagcttgtc catcttgtcg agcgccatgc cgcagccgcg
     3481 cgcgacgcag gtcagcggtt cctcggccac gaccaccggc aggccggtct cttccatcag
     3541 caggcggtcg agatcgcgca ccagggcgcc accgccggtg agcatcatgc cgcgctcggc
     3601 gatgtcggcg ccgagctcgg gcggcgtctg ctcgaggccg atcttgaccg cggagacgat
     3661 ctggttgagc ggctcggtga gcgcctcgag gatctcgttg gacgagatcg tgaagctgcg
     3721 cgggatgccc tcggccaggt tgcggccctt gacctccatc tcgcgcacct cggaaccggg
     3781 gaaggcggaa ccgatctgct tcttgatctt ctcggcggtg gtttcgccga tcagcatgcc
     3841 gtagttgcgg cggatgtaat tgacga
//
LOCUS       DAOQNN010000308         3864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_15492_length_3864_cov_9.751116, whole genome shotgun sequence.
ACCESSION   DAOQNN010000308 DAOQNN010000000
VERSION     DAOQNN010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15492_length_3864_cov_9.751116"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKV97_13490"
     CDS             complement(<1..202)
                     /locus_tag="PKV97_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB06940.1"
                     /translation="MQKKGLIGSAFAMGVLSLAMSSAWAQEAPKLTAEEMEKGKQIYF
                     ERCAGCHGVLRKGATGKNLEPHW"
     gene            456..1349
                     /gene="cobA"
                     /locus_tag="PKV97_13495"
     CDS             456..1349
                     /gene="cobA"
                     /locus_tag="PKV97_13495"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNB06941.1"
                     /translation="MQAGTFRSDASPAGAAKPRSLRASRAALPPRGTPEGIGERPRST
                     RAQGHGKVHLVGAGPGDPDLLTVKALRLITGAEVVVYDHLVGEAVVALIPPAARRLYA
                     GKEAGNHALAQHEINRLLVELAREGHDVVRLKGGDPFIFGRGGEEMQALLEAGVECEI
                     VPGITAAAGAASATGIPLTHREHAQTLVFATGHLKDDTVDLDWAALARPRQTVVVYMG
                     LGALEIICTQMISHGLAADTPAAVIHAATTPRQCGVHATLATLPGAVRAAGIRAPALI
                     MIGEVVALDPGALLAAAATAG"
     gene            1402..2844
                     /locus_tag="PKV97_13500"
     CDS             1402..2844
                     /locus_tag="PKV97_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HNB06942.1"
                     /translation="MAGENEFFRPIREIEQRRVVSCSADDALVDIVGRMREMSISCVV
                     VVDGAHPTAILTDRDLRNKVIAAGRDPAGLRVRDVMSAPVITIGEDDVLYEALYRMSR
                     HGIHRLVVVDRKGALAGIVTVTDLLRLQAHSPHQLVLDIEKAESIDALRELHQRIQQL
                     IVHLAGTGIAIRDTVKLIANLNDQVLIRLIDLLRRDRYPDLTTDFAFVVMGSEGRGEQ
                     TLSTDQDNAILYADSVQGEALARLEAFSNELIDALISIGVPPCPGGIMARNPAWRRSL
                     TAWKRELTQWLSTPTPDNVMTGSMFMDLRPLYGRRELADTLREHALHYMSHEQGFLVR
                     MAQNMVNFAPPLGWFGRIKVEKSGERRGEVDLKKAGIFAITDGIKALAMEARRLDGST
                     HDRMEALVEAGVLKPEQVADLRAAFDFLVRMRLRGQVEALRGGRKPGNYIALDQLNAM
                     QQGELRLALEGVASFQAFIQQHFKLQLLRN"
     gene            complement(2880..3794)
                     /locus_tag="PKV97_13505"
     CDS             complement(2880..3794)
                     /locus_tag="PKV97_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNB06943.1"
                     /translation="MTEARPSILQYWSAVIRTTVKLWLEAQVFVHAAALAFFTVFSVA
                     PVVIIAVSLVGLVLGESAAQGRIAEQLEVAIGPEVAAAIQTAVVNSRIQHSGLLPTIA
                     GVGAIVFGATTVFTQMQESLNAIWGVAPRPTRSGVFIHLKTRLLSLAVVLGIGFVLLV
                     SLSLSIFVRALVAFAQDWLPVPVGLIIGLDWAIALLVVTLLFATIFRVLPEVVLGWRD
                     VLPGAFVTALLFALGRALIAIYLSTTATASTYGAAGSLVLLLLWVNYSSLILLFGAAF
                     TRAYLEARGRTVRPRATAVLVHRQLIED"
BASE COUNT          671 a         1402 c         1252 g          539 t
ORIGIN      
        1 tccagtgcgg ctcgaggttc ttgccggtgg cgcccttgcg cagcacgccg tggcaacctg
       61 cgcagcgctc gaagtagatc tgcttgcctt tttccatctc ctcggcggtc agcttgggcg
      121 cttcctgggc ccaggcgctg ctcatcgcga gggacagcac ccccatggca aacgccgatc
      181 cgatcaaacc tttcttttgc atagccctct ccttacacga cacgggatgt gaaacaacat
      241 tgcgacggca gtatggtttt tccggggcag gcccatcctt gatgtgaatt aattttccac
      301 accagcgccg aggataaagt tcggggacgc cggaacgacg tcgccatgcg cacgcccgcg
      361 gatttcgcgg tgtcctggac aagcgttttc cggcccctgg cggaaccgat ttttcgccgc
      421 aacggatttc ccgagaccgt gctggagccc acatcatgca ggcaggaacc tttcgatcgg
      481 atgcttcgcc ggccggcgcg gccaagccgc gctcccttcg cgccagtcgc gccgccctgc
      541 cgccccgcgg cacgcccgag ggcatcggcg agcggccgcg atccacgcgt gcgcagggac
      601 atggcaaggt ccatctggta ggcgccggcc cgggcgaccc cgaccttctc accgtgaagg
      661 cgctgcgcct gatcaccggc gccgaagtgg tcgtctacga ccatctcgtc ggcgaggcgg
      721 tggtggcgct gatcccgccc gccgcgcgtc gcctgtacgc gggcaaggaa gccggcaacc
      781 acgccctcgc ccagcacgag atcaaccgcc tgctcgtcga actcgcacgc gaagggcacg
      841 acgtggtgcg actcaagggc ggcgatccct tcatcttcgg gcgcggcggc gaagagatgc
      901 aggccctgct cgaggccggc gtcgagtgcg agatcgtgcc cggcatcacc gccgcggcgg
      961 gcgcggcctc ggccaccgga atcccgctca cccaccgcga gcacgcgcag accctggtgt
     1021 tcgccaccgg ccacctcaag gacgacaccg tcgacctcga ctgggcggcg ctcgcccgcc
     1081 cgcgccagac cgtggtggtc tacatgggcc tcggcgcgct cgagatcatc tgtacgcaga
     1141 tgatctccca cggactggcg gcggacacgc cggcggcggt gatccacgcc gccaccacac
     1201 cgcggcagtg cggcgtgcat gccacgctcg cgaccctgcc cggggcagtg cgcgcggccg
     1261 gcatccgcgc gccggcgctg atcatgatcg gcgaggtggt cgcactcgac cccggcgcgc
     1321 tgctcgcagc cgcggcgacg gcgggctgaa acggccgcgc ccgggtaaaa tggcgaattc
     1381 gacgcacccc ggggagcagc catggcagga gaaaacgagt tctttcgtcc gatccgggag
     1441 atcgagcagc gccgcgtggt gagctgcagc gccgacgacg cgctggtcga tatcgtcggc
     1501 cgcatgcgcg agatgagcat ctcctgcgtg gtcgtcgtcg acggcgcgca cccgacggcg
     1561 atcctgaccg accgcgacct gcgcaacaag gtgatcgccg ctgggcgcga cccggcgggg
     1621 ctccgggtgc gcgacgtgat gagcgcgccg gtgatcacca tcggcgagga cgacgtgctc
     1681 tacgaggcgc tctaccgcat gtcgcgccac ggcatccacc gcctcgtcgt ggtcgaccgc
     1741 aagggcgcac tcgccggcat cgtcaccgtc accgacctgc tgcgcctgca ggcgcactcg
     1801 ccccaccagc tcgtgctcga catcgagaag gccgaatcga tcgacgccct gcgcgagctg
     1861 catcagcgca tccagcagct gatcgtgcat ctcgccggca ccggcatcgc aatccgcgac
     1921 acggtgaagc tgatcgccaa cctcaacgac caggtcctga tccgcctgat cgacctgctg
     1981 cgccgcgatc gctaccccga tctcaccacc gacttcgcct tcgtggtgat gggcagcgaa
     2041 gggcgcggcg aacagacgct atcgaccgac caggacaacg ccatccttta cgccgactcg
     2101 gtgcagggcg aggcgctggc gcggctcgaa gccttctcca acgaactcat cgacgccctg
     2161 atctcgatcg gcgtgccacc ctgccccggc ggcatcatgg cgcgcaaccc cgcctggcgg
     2221 cgcagtctga cggcctggaa gcgcgagctc acgcagtggc tatcgacgcc gacgcccgac
     2281 aacgtgatga ccggcagcat gttcatggac ctgcgcccgc tctacggtcg gcgggagctc
     2341 gccgacaccc tgcgcgaaca cgcgctgcac tacatgagcc acgagcaggg cttcctcgtg
     2401 cggatggcgc agaacatggt caacttcgcc ccgccgctgg gctggttcgg ccgcatcaag
     2461 gtggaaaaat ccggcgagcg ccgcggcgag gtcgacctca agaaggccgg catcttcgcc
     2521 atcaccgacg gcatcaaggc gctggcgatg gaggcgcgcc ggctcgacgg cagcacccac
     2581 gaccgcatgg aggcgctggt cgaggccggc gtgctcaagc ccgaacaggt cgccgacctg
     2641 cgcgccgcct tcgacttcct ggtgcggatg cgactgcgcg gccaggtcga agccttgcgc
     2701 ggcgggcgca agccgggcaa ctacatcgcc ctcgaccagt tgaacgcgat gcagcagggc
     2761 gagctgcgcc tcgccctgga gggcgtcgcc agcttccagg ccttcatcca gcagcacttc
     2821 aagctgcagc tgctgcgcaa ctgagcggag cgcggccgag ccacccacgg gaggcggact
     2881 caatcctcga tcagctggcg atgcaccagc accgccgtcg cgcgcggccg caccgtccgg
     2941 ccgcgcgcct ccagataggc gcgcgtgaac gcggcgccga agagcaggat caacgaggag
     3001 taattcaccc acagcagcag cagcaccagc gagcccgccg ccccgtaagt ggacgcggtc
     3061 gcggtggtcg acaggtagat cgcgatcagc gcgcgcccga gcgcgaacag cagcgcggtg
     3121 acgaaggcgc ccggcagcac gtcacgccag cccagcacca cctcgggcag cacgcggaag
     3181 atggtggcga agagcagcgt caccaccagc agcgcgatcg cccagtccag cccgatgatc
     3241 agccccaccg gcaccggcag ccagtcctgc gcgaaggcga cgagcgcgcg cacgaagatg
     3301 ctcagcgaca gcgacaccag cagcacgaag ccgatgccga gcaccaccgc gagcgacagg
     3361 aggcgcgtct tgagatggat gaacacgccg ctgcgcgtgg gccgcggcgc cactccccag
     3421 atcgcgttga gcgattcctg catctgggtg aataccgtcg tcgcgccgaa cacgatggca
     3481 ccgacgccgg cgatcgtggg cagcagcccg ctgtgctgga tgcggctgtt gaccaccgcg
     3541 gtctggatcg cagccgccac ctccggcccg atcgccacct cgagctgctc ggcgatgcgg
     3601 ccctgagccg cactctcgcc gagcaccagg cccaccaggc tgaccgcgat gatcaccacc
     3661 ggcgccaccg agaacacggt gaagaacgcc agcgcagccg catgtacgaa gacctgggct
     3721 tccagccaca gcttcacggt ggtccggatc acggcggacc agtattgcag gatcgacggt
     3781 cgggcttcgg tcatggccgc atgttagcgc cattgccctg gaagccgctt ccagaacgcg
     3841 cccctcagct cactcccact cgat
//
LOCUS       DAOQNN010000309         3843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_15626_length_3843_cov_7.169747, whole genome shotgun sequence.
ACCESSION   DAOQNN010000309 DAOQNN010000000
VERSION     DAOQNN010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15626_length_3843_cov_7.169747"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..250
                     /locus_tag="PKV97_13510"
     CDS             53..250
                     /locus_tag="PKV97_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06944.1"
                     /translation="MTTQGDAARDLARMATEADDEATRTLARLLFYQDTSAAERDPQL
                     AQLAATGDTIRLDELLAKLGA"
     gene            254..823
                     /locus_tag="PKV97_13515"
     CDS             254..823
                     /locus_tag="PKV97_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06945.1"
                     /translation="MNVPQHRDPIARLALFALVAVLAGALFVPAQPVTTAAAQPIVIL
                     STVTPAIVPLASEVMPTAASVEAAPTALPIIPTAEVARIYPATATPISTEPPPPAIEV
                     ASAPETAPAAPPPIDAALNDPAQNGGTVPDPGCPFPIVNGVCGDGNPAPPAQTEFGSK
                     PGRDIPPKVDRLKAIDLPGGGAIYPTAQP"
     gene            820..984
                     /locus_tag="PKV97_13520"
     CDS             820..984
                     /locus_tag="PKV97_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06946.1"
                     /translation="MKTARLLLVYKDGREVIEGVTGYRETKGDRLEIFRGGKPPLYVP
                     LETLTSYGYV"
     gene            981..2276
                     /locus_tag="PKV97_13525"
     CDS             981..2276
                     /locus_tag="PKV97_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06947.1"
                     /translation="MIMLTVITSYNLHIAVLLGLILMARAATHWRPALVIDALAVALY
                     AVRPVPATLVFIPVQALIRYVPELARQVALAFRVQSPHGWTRALLLLLLPGLARYDAP
                     AAAPATTAPSSYPPAVTGATTRLPEPSISAARPLGPGEWIAALNEDEAAPHLGIIGPP
                     RLGKTTLALVLVSRRPGDLVIATPKAADDADAWGGATCARPTIDLASQAVDWTPIAQA
                     IGDVHFEMLRRNVENDVGAPPLTIVIDELSTTIPNIPPVARRQLVELWAMAPSCKIRV
                     IVISQDVNARAWGVEGRRDILDSLVFARVAPGRRWSLGRLDPNGRLTDPTPLDTAGLD
                     RQARAVRLRGREWGGRRQGAPCPVGAGVGAPPPPIPAQTNAHTQTQTDAEARIALYME
                     WRAAGMKKDMARALRNAKGAGIDDVEWAEAGKRLANGPS"
     gene            2285..2926
                     /locus_tag="PKV97_13530"
     CDS             2285..2926
                     /locus_tag="PKV97_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06948.1"
                     /translation="MKHLIKLALFCLAAVAAYLVYGFTALVAPWYVALGAAGSLVGTY
                     IGLAFADVPAAQRPRAIMVARGAMVVEAVYGFLFMLSVQSPEVFHTPLSLYLSVPLSI
                     LHGAAFSVLAYFVSLFVVHEARGVPDAAPIEPRDQAIVDALGAVVEVSRAVLARLDEP
                     RSIAAPEPIQAVPLLEQDVDQTIQVGGKAVSLRKLAEISGKPLTSLRREIEKL"
     gene            3097..3390
                     /locus_tag="PKV97_13535"
     CDS             3097..3390
                     /locus_tag="PKV97_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06949.1"
                     /translation="MKTITIQIDTTDEAMVGGFGWENCDPRASVLEFKAQVLKRVGER
                     FPGYAIEIEETDHANTVVFADGRDYSREVEEDQQAVREIISSVWADAEWYVEA"
     gene            3391..3696
                     /locus_tag="PKV97_13540"
     CDS             3391..3696
                     /locus_tag="PKV97_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06950.1"
                     /translation="MTDRIDAALALLAPHLSATDPLEVRAIVEDLHDSAQDDLLASLR
                     GANEAAQAWGVSQRRAVAHIARLHEKHGIGRQVGGAWVLRQVDIERHRPDPKYRRKA"
BASE COUNT          645 a         1319 c         1315 g          564 t
ORIGIN      
        1 acggcgtgaa tggttcgcac cccgcaagat ggaaccgaga ggagtatagc atatgaccac
       61 ccagggcgac gccgcgcgag acctggcgcg gatggcaacc gaggccgacg acgaggcaac
      121 gcgcacgctc gcgcggctgc tgttctacca ggacacgagc gccgccgaac gtgacccgca
      181 gctcgcccag ctcgccgcga cgggtgacac catccggctg gacgagctgc tggcgaagct
      241 aggagcatag cgtatgaacg tcccccagca ccgcgacccg atcgcccgcc tggcgctgtt
      301 cgcgcttgtg gccgtgctgg ccggcgcgct gttcgtgccg gcgcaacccg tcacgacggc
      361 ggcggcgcag ccgatcgtca tcctctcgac tgtgaccccg gcgatcgtgc cgctggcctc
      421 cgaggtgatg ccaaccgccg cgtcagtcga ggccgcgccg accgcgcttc cgatcatccc
      481 gaccgccgag gtagcgcgga tctacccggc gacggcgacg cccatttcga ccgagccgcc
      541 gccgcccgcg atcgaagtcg ccagcgcgcc ggagacggcc ccggccgcgc cgccgccgat
      601 cgacgcggcg ctgaacgacc ccgcgcagaa cggcggcacc gtgcccgatc ccggctgccc
      661 gttcccgatc gtgaatggcg tctgcggcga cggcaaccca gcaccgccgg cgcagaccga
      721 gttcgggagc aagccgggcc gcgacattcc gccgaaggtc gatcgcctca aggcgatcga
      781 cctgccgggc gggggcgcca tctacccgac ggcgcaacca tgaaaaccgc gcgcctgctg
      841 ctggtctaca aagacggccg ggaagtgatc gagggcgtga ccggctaccg cgagacgaaa
      901 ggcgaccggc tagagatttt ccgcggcggc aaaccaccgc tgtatgtgcc gctcgaaaca
      961 ctcacgagct acgggtacgt atgatcatgc taactgtcat caccagttac aacctgcata
     1021 ttgccgtgct gctcggcctc atcctgatgg cacgcgcggc gacgcactgg cggccggcgc
     1081 tggtgatcga cgcactggcg gtcgcgctct acgccgtgcg gcccgtgccg gcgacgctgg
     1141 tgtttatccc ggtgcaagcg ctcatccgct acgtgccgga gctggcgcgc caggtcgcgc
     1201 tcgcgttccg cgtccagtcg ccgcacggtt ggacgcgcgc gctgctgctg ctactgctgc
     1261 caggtctcgc gcgctacgac gcgccagccg cggccccggc gacgacggcg ccgagcagct
     1321 acccgcccgc cgtgacgggc gcgaccacgc gcctgccaga gccgagcatt tcggccgcgc
     1381 gcccgctcgg cccaggcgaa tggatcgccg cgctgaacga ggacgaggcc gcgccgcatc
     1441 tcggcatcat cggcccgccg cggctcggca aaacgacgct cgcactggtg ctggtttcgc
     1501 gccgaccggg cgacctggtg atcgccacgc caaaggccgc cgacgacgcg gatgcatggg
     1561 gcggcgcgac atgcgcgcga ccgacgatcg atctggcctc gcaggcggtc gattggacgc
     1621 cgatcgcaca ggccattggc gacgtgcatt tcgaaatgct ccgtcgcaac gttgagaacg
     1681 acgtgggggc gccgccgctc acgatcgtga ttgacgagct gagtacgacg attccaaaca
     1741 tcccgccggt tgcgcgccgc cagcttgtcg agctgtgggc aatggcgccg agctgcaaga
     1801 tccgggtgat cgtcatcagc caggacgtca acgcccgcgc gtggggggtc gaggggcgcc
     1861 gggacatcct cgactccctg gtgttcgccc gcgtggcgcc cggccggcgg tggtccctgg
     1921 gacgactgga ccccaacggg cgcctgacag acccaacccc cctcgacacc gccgggcttg
     1981 accggcaggc ccgcgccgtc cggctccggg ggcgggagtg gggcggacgc cggcaggggg
     2041 cgccgtgccc cgtgggtgcg ggtgtggggg cgccccctcc ccccatccca gcgcaaacaa
     2101 acgcacacac acaaacgcaa acagacgccg aggcgcggat cgcgctctat atggaatggc
     2161 gcgcggcggg catgaaaaag gacatggcgc gcgcgctccg caacgcaaag ggcgcgggta
     2221 ttgacgatgt ggagtgggcc gaggcgggca aacggctcgc aaacgggcca agttaggagc
     2281 aactatgaag cacttaatca agctggcctt gttctgcctg gcggccgtcg ccgcctatct
     2341 ggtctacggc ttcaccgccc tggtcgcgcc gtggtacgtg gcgcttggcg cggccggatc
     2401 gctggtcggc acgtacatcg gcctggcatt cgcggatgtg ccagccgcgc agcgcccgcg
     2461 cgccattatg gtcgcgcggg gggccatggt ggtcgaggcg gtgtacgggt tcctgttcat
     2521 gctgagcgtg cagtcgcccg aagtgttcca cacgccgctc agcctgtacc tgagtgtgcc
     2581 actgtcgatc ctgcatggcg cggcgttctc ggtcctggcc tacttcgtga gcctgttcgt
     2641 ggtccatgag gcgcgcggtg tgccagacgc cgcgccaatt gagccacgcg accaggcgat
     2701 cgtggatgcg cttggcgcgg tggtcgaggt gagccgcgcc gtactggcac gcctggacga
     2761 gccgcgatcc atcgccgcgc cagaaccgat ccaagccgtg ccactgttgg aacaggacgt
     2821 ggatcagacg atccaggttg gcgggaaggc ggtcagccta cgcaagctgg ccgagatcag
     2881 cgggaagccg ctgaccagcc tgcgccggga gatcgagaaa ctttagcccc ctggcagccg
     2941 cactaggccc cgcgcctgcc gcctctcccc cgagcccccg cccgccgtga tggcgggctt
     3001 ttctcttttg gaaaaaagat ctacttgaca aactgtactc aatctggtat agtacaacca
     3061 taccggatga cgaatagtta cacaaaggag cgcatcatga agaccatcac gattcagatc
     3121 gatacgaccg acgaggcgat ggtgggcggg ttcggctggg agaattgcga cccgcgcgca
     3181 agcgttctgg agttcaaggc gcaggtgctc aagcgggtcg gcgagcgctt ccccggctac
     3241 gcgatcgaga tcgaggaaac cgaccacgcg aacacggttg tcttcgcgga cgggcgcgac
     3301 tacagccgcg aggtggaaga ggaccagcag gcggtgcgcg agatcatttc aagcgtgtgg
     3361 gccgacgccg agtggtacgt ggaggcatag atgaccgatc gcatcgacgc ggcgctagcc
     3421 ctgctggcgc cgcatctctc cgccaccgac ccgttggagg tccgcgcgat tgtcgaggat
     3481 ctgcacgaca gcgcacagga tgacctactc gccagcctgc gcggcgccaa tgaggccgcg
     3541 caggcgtggg gggtgagcca gcggcgcgcg gtcgcgcata tcgcgcggct gcatgaaaag
     3601 cacggcatcg gtcgccaggt tggcggggcg tgggtgctcc gccaggtgga tattgagcgg
     3661 catcgcccgg acccgaagta tcgacggaaa gcctagcccc gcctcgccgc gctggcgcga
     3721 cacgcccccg atccgcatcg gatcgggggc gttttgttta ccctaagagc catgctttca
     3781 gcgcctgccg cacggcttcc cacgcgccac cgccgcctga tccgtcgccg ggcgggccct
     3841 gcg
//
LOCUS       DAOQNN010000310         3810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_15860_length_3810_cov_9.169907, whole genome shotgun sequence.
ACCESSION   DAOQNN010000310 DAOQNN010000000
VERSION     DAOQNN010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15860_length_3810_cov_9.169907"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="PKV97_13545"
     CDS             <1..1267
                     /locus_tag="PKV97_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNB06951.1"
                     /translation="RLGNMEAVQFHPTAIVPAGILVTEGCRGDGGLLRDVDGHRFMPD
                     YEPEKAELASRDVVSRRMAEHMRRGKGVKSAAGEHLWLDIAVLGAAHIDHKLREVKDI
                     CRHFLGIDPARDWIPVRPTQHYSMGGIRTDHRGESPALRGLFAVGEAACWDLHGFNRL
                     GGNSLAETVVAGMLVGEYVADFVESHAGALHFSPGLAREFVGRERAALEAIAARPVSA
                     PGGDSVASLRREMEATMTEHVGLFRNETGLRTAVATLTDLHARAQRVGIASRHLDANP
                     ELVAAYRLPRMLALALCAAQGALERRESRGAHAREDYPARNDRDWLRRTLAWWPVGAA
                     TPVLEYEPIRVESMELPPGFRGYGTRNIVEHPATALREAELESIRARLEGAGGAPLQA
                     ALLDFRARLPERYRGDNERIADIEEGAPR"
     gene            1264..2001
                     /locus_tag="PKV97_13550"
     CDS             1264..2001
                     /locus_tag="PKV97_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HNB06952.1"
                     /translation="MSRRLKISIFRHNPRDPRSVPRLVDYELDERPGMTLFIALNAIR
                     DSQAPDLNFDFVCRAGVCGACAMVIDGRPGLACRTLTSTLPAAFSLYPLPVFRLVADL
                     SVDTGSWMRELAERLQTWVHLDAEPDLDRLEARMEPALAEAVYELDRCIECGCCIAAC
                     ATAQMREGFVGAVGLEKIARFRLDPADTRDDADFYELVGNDEGIFGCMSLLACNDLCP
                     KELPLQTQLAWLRRKMAGQGVRWLRRG"
     gene            complement(2470..3654)
                     /locus_tag="PKV97_13555"
     CDS             complement(2470..3654)
                     /locus_tag="PKV97_13555"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNB06953.1"
                     /translation="MQVKKLTELDVRGKKVFIRADLNVPQDDAGNITEDTRIRASLPS
                     IQYCLDNGAAVMVTSHLGRPTEGTVGPDDTLAPVAVRLGQLLHRPVRLVSDWVEGGFE
                     VKPGEVVLLENCRCNKGEKKDNEELAKKMAALCDVYVNDAFGTAHRAEATTHGIARFA
                     PVACAGILMGAEIDALGKALQSPQRPLVAIVGGSKVSSKLTILKSLAHKVDQLIVGGG
                     IANTFLLASGKRIGESLAEPELVKEAQAIMDMMKARGAEVPLPVDVVVADEVSALARA
                     NRIPVEEVGAHDRILDVGPKTAAKLSEIIANAGTIVWNGPVGVFELPQFAGGTKMMAS
                     AIAHSEAFSIAGGGDTLAAIAKFHIAEDVGYISTGGGAFLEFLEGKTLPAIAALEARV
                     KD"
BASE COUNT          535 a         1341 c         1366 g          568 t
ORIGIN      
        1 gcgcctgggc aacatggagg cggtgcagtt ccaccccacc gcgatcgtgc ccgccggcat
       61 tctcgtcacc gagggctgcc gcggcgacgg cggcctgctg cgcgacgtcg acggccaccg
      121 cttcatgccc gactacgagc cggagaaggc cgagctggcc tcgcgtgacg tggtctcgcg
      181 ccgcatggcc gagcacatgc gccgcggcaa gggcgtcaag tcggccgccg gcgagcacct
      241 gtggctggac atcgcggtgc tcggcgccgc ccatatcgac cacaagctgc gcgaggtgaa
      301 ggacatctgc cgccacttcc tcggcatcga cccggcgcgc gactggatcc cggtgcggcc
      361 gacccagcac tactcgatgg gcggcatccg caccgaccac cgcggcgagt cgcccgcgct
      421 gcgcggcctg ttcgcggtgg gcgaggcggc gtgctgggac ctgcacggct tcaaccgcct
      481 gggcggcaac tcgctcgccg agacggtggt ggcgggcatg ctggtgggcg agtacgtcgc
      541 cgacttcgtc gagtcgcacg ccggcgcctt gcacttctcg cccggcctcg cgcgcgagtt
      601 cgtcggccgc gagcgcgccg ccctggaggc gatcgcggct cgtccggtgt ccgcgccggg
      661 cggcgacagc gtggcctcgc tgcggcgcga gatggaggcg accatgaccg agcacgtcgg
      721 cctgttccgc aacgagaccg ggctgcgcac agcggtcgcg acgctgaccg acctgcatgc
      781 gcgcgcgcag cgggtcggca tcgccagccg ccacctcgac gccaatccgg agctggtggc
      841 cgcctaccgg ctgccgcgca tgctggcgtt ggcgctgtgc gccgcgcagg gcgcgctcga
      901 gcgccgcgag agccgcggtg cgcacgcgcg cgaagactac ccggcacgca acgaccgcga
      961 ctggctgcgc cgcacgctgg cctggtggcc ggtgggcgcc gccacgccgg tgctggaata
     1021 cgagccgatc cgggtcgaga gcatggagct gccgcccgga ttccgcggct atggcacgcg
     1081 caacatcgtc gagcatccgg ccaccgcgct gcgcgaggcc gagctcgagt ccatccgtgc
     1141 ccgtctcgaa ggcgcggggg gtgccccgct gcaggccgcg ctgctggatt tccgcgcacg
     1201 cctgcccgaa cgctatcgcg gcgacaacga acgcatcgcc gacatcgaag agggagcgcc
     1261 gcgatgagcc gccgcctgaa gatctcgatc ttccgccaca acccgcgcga cccccggagc
     1321 gtgccgcgcc tggtcgacta cgagctcgac gagcggccgg gcatgacgct cttcatcgcg
     1381 ctcaacgcga tccgcgactc ccaggcgccc gacctcaact tcgacttcgt gtgccgcgcc
     1441 ggcgtgtgcg gggcgtgcgc gatggtgatc gacggccgcc ccgggctggc ctgccgcacg
     1501 ctgacgagca cgctgccggc ggcgttcagc ctctatccgc tgccggtgtt ccgcctcgtg
     1561 gcggacctgt cggtggatac cgggagctgg atgcgcgagc tcgccgagcg cctgcagacc
     1621 tgggtgcatc tcgacgcgga gcccgatctc gatcgcctgg aggcgcgcat ggagccggcg
     1681 ctggccgagg cggtctatga gctcgaccgc tgcatcgaat gcggctgctg catcgccgcc
     1741 tgcgccaccg cgcagatgcg cgagggcttc gtcggcgcgg tcggtctcga gaagatcgcg
     1801 cgtttccgcc tcgacccggc ggatacgcgc gacgacgccg acttctacga actcgtcggc
     1861 aacgacgagg gcatcttcgg ctgcatgagc ctgctcgcct gcaacgacct ctgtcccaag
     1921 gagctgcccc tgcagaccca gctcgcctgg ctgcgccgca agatggcggg gcaaggggta
     1981 cgctggctgc ggcggggata aatcgagcgc cgcaatcgcg actcgcgtcg ctcctacacc
     2041 tcatccgcgg cgccgcagga cgcgtaggag cgacgccagt cgcgatccgg cgcaataggc
     2101 cagcgcggcc tgaactccgg gcgccgcaat cgcgactcgc gtcgctccta cgcccccacc
     2161 cgagacgccg cgggtcttgc aggagcgacg ccagtcgcga tcccggccac gcggtatttg
     2221 caggccgtgc gtcgcccgcc ggagcgcccg atcgcgactt gcgtcgctcc cacgacaacc
     2281 gtcgcgctcc ggccagcgcc tggaattgcg tgtaggagcg acgccagtcg cgatccggcg
     2341 caataggcca gcgcggcctg aactccgggc gccgcaatcg cgactggcgt cgctcctgca
     2401 ccccacccga gacgccgcgg gtcttgcagg cgcgacgcaa gtcgcaatcg ggcggtgccg
     2461 gatcggggct cagtccttga cgcgcgcttc cagtgccgcg atcgcgggca gggtcttgcc
     2521 ctcgaggaac tcgaggaagg cgccgccgcc ggtggagatg tagcccacgt cctcggcgat
     2581 gtggaacttg gcgatcgccg ccagggtgtc gccgccaccc gcgatcgaga aggcctcgga
     2641 gtgcgcgatc gccgaggcca tcatcttggt gccgccggcg aactggggca gctcgaacac
     2701 gccgaccggg ccgttccaca cgatggtgcc ggcgttggcg atgatctcgg agagtttggc
     2761 ggccgtcttc gggccgacgt cgaggatgcg gtcgtgcgcg ccgacctctt ccaccggaat
     2821 gcggttggca cgcgccagcg cggagacctc gtcggcgacg acgacgtcga ccggcagcgg
     2881 cacctcggcg ccacgcgcct tcatcatgtc catgatcgcc tgcgcttcct tgaccagctc
     2941 gggctcggcg agcgactcgc cgatgcgctt gccggaggcg agcaggaagg tgttggcgat
     3001 gccgccgccg acgatgagct ggtccacctt gtgggcgagc gacttcagga tggtgagctt
     3061 ggaggagacc ttggagccgc cgacgatcgc caccagcgga cgctgcgggg actgcagggc
     3121 cttgccgagc gcgtcgatct cggcgcccat caggatgccg gcgcaggcca ccggggcgaa
     3181 gcgggcgatg ccgtgggtgg tggcctcggc gcggtgggcg gtgccgaagg cgtcattgac
     3241 atagacgtcg cagagcgcgg ccatcttctt cgccagctct tcgttgtcct tcttctcgcc
     3301 cttgttgcag cggcagttct cgagcaggac gacctcgccc ggcttgacct cgaagccgcc
     3361 ctcgacccag tcggacacca ggcgcaccgg gcggtgcagc agctggccga ggcgcaccgc
     3421 gaccggggcc agggtgtcgt cggggccgac cgtgccttcg gtggggcggc cgaggtggga
     3481 ggtgaccatc accgcggcgc cgttgtccag gcagtactgg atcgagggca gcgaggcgcg
     3541 gatgcgggtg tcctcggtga tgttgccggc gtcgtcctgc ggcacgttga ggtcggcgcg
     3601 gatgaagacc ttcttgccgc gcacgtcgag ttcggtgagt ttcttgactt gcatgattcg
     3661 ctcgttcccg gaaaaacgcc ctgcggggca gggcgtcgat tcgactggat gtccgtgtgt
     3721 agcctggcgc ttacttggcg ttgcagaagg cgcgcgcggc gtcgagcatg cggcaggagt
     3781 agccccactc gttgtcgtac caggccagca
//
LOCUS       DAOQNN010000311         3776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16090_length_3776_cov_9.239989, whole genome shotgun sequence.
ACCESSION   DAOQNN010000311 DAOQNN010000000
VERSION     DAOQNN010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16090_length_3776_cov_9.239989"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /gene="acsA"
                     /locus_tag="PKV97_13560"
     CDS             <1..1461
                     /gene="acsA"
                     /locus_tag="PKV97_13560"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNB06954.1"
                     /translation="RGERVFVLTGRIPELYIAALGGLKAGCVVSPLFSAFGPEPIATR
                     IGIGEGAVLVTTDLLYERKVAKLRDRLATLRHVLLVGEEGGATAIPGTGDLATLMAQA
                     ADTFETVATQPEDLALLHFTSGTTGTPKGAMHVHGAVLTHWATGRYALDLHEGDIFWC
                     TADPGWVTGTSYGIVAPLLHGVTSIVDVADFDAERWYRILQEQQVSVWYTAPTAIRML
                     MKAGPGLARGFSFPHLRFVASVGEPLNPEAVWWGQEVLGRPIHDNWWQTETGGIMIAN
                     LPALDIKPGSMGKPLPGVEAAIVRPREGGGVEVIDAPDQEGELALRRGWPSMLRGYLN
                     NEERYRKCFAGDWYLTGDLARRDADGYYWFVGRSDDVIKSAGHLIGPFEVESALMEHP
                     AVAEAGVIGKPDEMVGEVVKAFVSLKQGFEPSEALRKELLGHARKKLGAAVAPKEIDF
                     LPVLPRTRSGKIMRRLLKARELGLPEGDTSTLEAGA"
     gene            1458..2471
                     /gene="pdhA"
                     /locus_tag="PKV97_13565"
     CDS             1458..2471
                     /gene="pdhA"
                     /locus_tag="PKV97_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNB06955.1"
                     /translation="MTRTRKPRASAERPLELRLLADMLRIRRMEEKCAELYGAGRIRG
                     FLHLYIGEEACATGAMHALAADDNVVATYREHGHALLRGVGMDAIMAEMFGKAAGCSR
                     GRGGSMHLFDVARRFYGGNAIVGGGLPLAVGLALAERLQGVRRVTLCVFGEGAMAEGA
                     FHESINLAALWQLPVLFCCENNLYAMGTALARSASQTDLCAKAASYRVATFRADGMDV
                     RAVFETVSRAVRQVREGGGPAFVELQTYRFRAHSMFDPDLYRDKAEVERWKQRGPIHS
                     LSAQLKAEGRLDEEAFLALDAEAEAEVAAAVAFAEQAPWEPVADLLKDVHTPAGAAAG
                     GTP"
     gene            2468..3439
                     /locus_tag="PKV97_13570"
     CDS             2468..3439
                     /locus_tag="PKV97_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNB06956.1"
                     /translation="MRMSYREALRLALREALQNDARVFLMGEDVGRYGGSYAVSKGLL
                     EEFGSERIRDTPLSELGFVGAGVGAALGGLRPIVEVMTVNFSLLALDQIVNSAAVLRH
                     MSGGQCSVPLVLRMATGAGRQLGAQHSHSLENWYAHIPGIRVLAPATVADARGMLAPA
                     LADPDPVVIFEHAQLYNLEDELPDDWRCDIARAAVRRAGAQASVFAYGGCLPKALQAA
                     EELAAQGVEVEVVDLRVLRPLDIDTVAASVRRTHRAVVVDEGWRSGSLAAELIARIVE
                     TCFYELDAPPARVCSEEVPIPYAKHLEEAALPQVPKIGAAVKELLNV"
     gene            3432..>3776
                     /locus_tag="PKV97_13575"
     CDS             3432..>3776
                     /locus_tag="PKV97_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNB06957.1"
                     /translation="MFEFKLPSLGADMDEGKLLEWLVRPGDRVVKGQVVAIVDTSKAA
                     VDVEIWQDGTVHELLVEPGTRMAVGTVMATLLEPGEAPAAKRTRTKTVKAAAAAAVPV
                     VEAPAAKPAPDVS"
BASE COUNT          559 a         1271 c         1400 g          546 t
ORIGIN      
        1 cgcggcgagc gtgtcttcgt gctgaccggg cgcatcccgg agctgtacat cgcggcgctc
       61 ggcggcctga aggcgggctg cgtggtgtcg ccgctgttct ccgccttcgg cccggagccg
      121 atcgccaccc ggatcggcat cggcgagggg gcggtgctgg tcaccaccga cctgctgtac
      181 gagcgcaagg tggcgaagct gcgcgatcgt ctggcgacct tacgccatgt gctgctggtg
      241 ggtgaggagg gtggcgcgac cgccatcccc ggcaccggag accttgcgac gctgatggcg
      301 caggccgcag acacgttcga gacggtggcg acccagcccg aagacctcgc cctgctgcac
      361 ttcaccagcg gcaccaccgg cacgcccaag ggcgccatgc acgtgcatgg cgcggtcctc
      421 acccactggg cgaccggccg ctacgcgctc gatctgcatg agggcgacat cttctggtgc
      481 accgccgacc cgggttgggt taccggcacc tcctacggca tcgtcgcgcc gctgctgcac
      541 ggcgtgactt ccatcgtcga tgtggccgac ttcgacgccg agcgctggta ccgcatcctg
      601 caggaacagc aggtcagcgt ctggtacacc gcacccacgg cgatccgcat gctgatgaag
      661 gccgggcccg gattggcgcg cggcttctcc ttcccccacc tgcgcttcgt cgccagcgtg
      721 ggcgaaccgc tcaaccccga ggcggtatgg tgggggcagg aagtgctcgg cagaccgatc
      781 cacgacaact ggtggcagac cgagaccggc ggcatcatga tcgccaacct gccggcgctc
      841 gacatcaagc ccggctcgat gggcaagccg ctgcccggcg tcgaggcggc catcgtgcgt
      901 ccgcgcgaag gcggcggcgt ggaggtgatc gacgcgcccg accaggaggg cgaactcgcg
      961 ctcaggcgtg gctggccgtc gatgctgcgc ggctacctca acaacgagga gcgctaccgc
     1021 aagtgcttcg ccggcgactg gtacctgacc ggcgacctgg cgcggcgcga cgccgacggc
     1081 tactactggt tcgtcggccg cagcgacgac gtgatcaagt cggccggcca cctgatcggg
     1141 cccttcgagg tcgagagcgc gctgatggag catccggcgg tggccgaggc cggcgtcatc
     1201 ggcaagcccg acgagatggt gggcgaggtg gtgaaggcct tcgtgtcgct gaagcagggc
     1261 ttcgagccct cggaggcgct gcgcaaggag ctgctcggcc atgcgcgcaa gaagctcggc
     1321 gcggcggtgg cacccaagga gatcgacttc ctgccggtgc tgccgcgcac gcgcagcggc
     1381 aagatcatgc gccggctgct gaaggcgcgc gaactcggcc tgcccgaagg cgacacctcc
     1441 acgctggagg ccggagcatg acgcgcacac gcaagcccag ggcctctgca gagcgtccgc
     1501 tggaactgcg cctgctggcc gacatgctgc gcatccgccg catggaggag aaatgcgccg
     1561 agctctacgg tgccggcagg atccgcggtt tcctgcacct ctacatcggc gaggaagcct
     1621 gcgccaccgg cgcgatgcac gcgctcgccg ccgacgataa cgtcgtcgcc acctaccgcg
     1681 agcacggcca cgcgctgctg cgcggtgtgg gcatggacgc gatcatggcc gagatgttcg
     1741 gcaaggcggc gggctgctcg cgcgggcgtg gcggctcgat gcatctgttc gacgtggcgc
     1801 gccgcttcta cggcggcaac gccatcgtcg gcggcggcct gccgctggcc gtgggcctgg
     1861 cgctggcgga gaggctgcaa ggcgtgcgcc gcgtcaccct gtgcgtgttc ggcgaaggcg
     1921 cgatggccga aggcgccttc cacgagtcga tcaacctcgc cgcgctgtgg cagcttccgg
     1981 tgctgttctg ctgcgagaac aacctgtacg cgatgggcac ggcattggcg cgctcggcgt
     2041 cgcagaccga cctgtgcgcc aaggcggcga gctaccgggt cgccaccttc cgcgccgacg
     2101 gcatggacgt gcgcgcggtg ttcgagaccg tgagccgcgc ggtgcggcag gtgagggagg
     2161 ggggcggccc ggccttcgtc gagctgcaga cctaccgctt ccgcgcgcac tcgatgttcg
     2221 accccgatct gtaccgcgac aaggcggaag tcgagcggtg gaagcagcgc ggtcccatcc
     2281 acagcctctc cgcgcagctg aaggccgagg gcaggctcga cgaggaggcc ttcctcgcgc
     2341 tcgatgccga ggccgaagcc gaggtggccg ccgcggtcgc gttcgccgaa caggcgccat
     2401 gggagccggt ggcggatctg ctgaaggacg tgcacacgcc ggccggcgcg gccgcagggg
     2461 gtacgccatg aggatgagct accgcgaggc gctgcggctg gcgctgcgcg aggcgctgca
     2521 gaacgatgcg cgcgtgttcc tgatgggcga ggacgtcggc cgctacggcg gcagctatgc
     2581 ggtgtcgaaa ggcctgctgg aggagttcgg ctccgagcgc atccgcgaca cgccgctgtc
     2641 cgaactcggc ttcgtcggcg ccggcgtggg ggcggcgctc ggcggcctgc gtccgatcgt
     2701 cgaggtgatg acggtcaatt tcagcctgct ggcgctggac cagatcgtca acagcgccgc
     2761 cgtgctgcgc cacatgtccg gcggccagtg ctcggtgccg ctggtgctgc gcatggccac
     2821 cggcgccggg cgccagctcg gcgcgcagca ctcgcacagc ctggagaact ggtacgccca
     2881 cattcccggc atccgcgtgc tggcgccggc taccgtggcc gacgcgcgcg gcatgctcgc
     2941 gccggcgctc gccgaccccg atccggtggt gatcttcgag cacgcccagc tctacaacct
     3001 ggaagacgag ttgccggacg actggcgctg cgacatcgcc cgcgccgcgg tgcgccgcgc
     3061 cggcgcgcag gcaagcgtgt tcgcttacgg tggctgcctc cccaaggcgc tgcaggcggc
     3121 ggaagaactg gcggcccagg gcgtcgaggt cgaggtggtc gatctgcgcg tgctgcgccc
     3181 gctggacatc gacaccgtcg ccgcatcggt gcgccggacg catcgtgcgg tggtggtgga
     3241 cgaaggctgg cgcagcggca gcctcgctgc cgagctgatc gcgcgcatcg tcgagacctg
     3301 cttctacgag ctcgacgcgc cgcccgcgcg ggtgtgcagc gaggaggtgc cgatcccgta
     3361 cgccaagcac ctcgaggagg ccgccttgcc tcaggtgccg aagatcggcg ccgcggtgaa
     3421 ggagctgctg aatgtttgag ttcaagctgc cttcgctcgg cgccgacatg gacgaaggca
     3481 agctgctgga gtggctcgtg cgccctggag acagggtcgt gaagggtcag gtcgtcgcca
     3541 tcgtcgacac ctccaaggcg gcggtggatg tcgagatctg gcaggacggc acggtgcatg
     3601 aactgctcgt ggagccgggg acgcggatgg cggtcggcac ggtgatggcg acgctgctgg
     3661 agcccggcga ggcgccggcg gcgaaaagga cgcgcacgaa gaccgtgaag gcggctgcag
     3721 cggcggcggt gccggtggtc gaagcgcccg cggcgaaacc cgcgccggac gtctcg
//
LOCUS       DAOQNN010000312         3773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16105_length_3773_cov_11.965573, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000312 DAOQNN010000000
VERSION     DAOQNN010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16105_length_3773_cov_11.965573"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2927
                     /locus_tag="PKV97_13580"
     CDS             <1..2927
                     /locus_tag="PKV97_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06958.1"
                     /translation="DPDAMAQAHGFGSGAEMLRAILNAPPRDEAVATRVRERLEAEEP
                     LADPQRFKAAVEKALHGRTALRLAATVLRAMMKGVRVSTSQLMAEAREAARVALLRTP
                     VGRINVRAMAAAEFRAAQRRDRAIASGKIEEAIEAQRQFLVQHYLTVEAAKVETEVRR
                     AVERSERNFGKADEQILKERRNLDYVNAGRLIMGRFGMLGSVPVERARADMEQMRELD
                     ADGFAKLERRMVDATSRAKAWREVALDEFRVLAADLDGLWSEAHRAEQVRIDGKMRDI
                     AEVRAELEDRLVRTFGGKRNEDGIVEAWEKVPRYLGGDIVSYMTRLEAFTRMVDGEAG
                     GPWHRYVYQPLKDATVAAELEKAEHKAWIESWLREFGPLSEKIDTKRFFGDGNALPTT
                     LAEVVGLLLHLGTESGERAVVLGNGLGHQVKGDLVTRLPVILRELADAGKITEKHFQQ
                     VQKLWDYIAKNLRERVFKTTREVWGFWPKEVEAKVVQTPFGTIPGGYFPAKVDHEKAT
                     VQTAAKLEALSKGGRDAFVAANPSVPRGMTIQRQNNVVQPRVLDLGMVGQHMDEAVNI
                     IHLQPAISDMARLFGGRRNTMAATLAQVLPKAQRNIIGPALERMARDTIEAPTNRTIR
                     GIVRWARKVASLAFLGFNPRNAAQQFTGLFNAVPEVGASRLWSAIREVAFHPGRTVEQ
                     IIGKSDAMRARWSDAMQLAAQDIQQILNPSTARKTLQQAQRAAFFLQRLVQQKTDAIV
                     WKAAYEKAVAELGPGMDVDKLDAEAVRRADSAVRLTQGEKTPLDVPTFLSGNAVAQMF
                     TQFADYPNTVLNQNIAAKDGQRFAVFAWTVLAPTLASSAIALTFAFGKIGSTRDQGDP
                     YEERLAKFIFGDQVRGIVGLVPIAGPLASSALNELFGLTNDPGGLRNQPFASWSMIQN
                     LANVATGDAKFSDALAAIAFGIGAPARPLVNVTRYQERIAAGREPQGDIVDYLRVLLN
                     GR"
     gene            complement(2914..3321)
                     /locus_tag="PKV97_13585"
     CDS             complement(2914..3321)
                     /locus_tag="PKV97_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06959.1"
                     /translation="MQLGHAHGAESSAALIAQTAQADRACFRLLMHTVLKASLGGSYR
                     EGLASASAVCWNPRMERRRQLRTALRLALAALCLLDAGWTCTAMMRPRRVSTMTWEQA
                     LSVAQRGDPAAALALSEHMREGIEAIRGLATAR"
BASE COUNT          627 a         1250 c         1364 g          532 t
ORIGIN      
        1 tggaccccga cgcgatggcg caggcgcacg gcttcggctc cggcgccgag atgctccgcg
       61 ccatcctgaa cgcgcccccg cgcgacgagg cggttgccac ccgcgtccgc gagcgcctgg
      121 aggccgagga gccgctggcc gacccgcagc ggttcaaggc ggccgtggag aaggcgctgc
      181 acggaaggac ggccctgcgg ctggcggcga ccgtgctgcg cgcgatgatg aagggcgttc
      241 gggtgtccac gtcgcagctc atggccgagg cgcgcgaggc ggcgcgggtg gcactgctgc
      301 ggacaccggt ggggcggatc aacgtgcgcg ccatggcggc ggccgagttc cgtgcggcgc
      361 agcggcggga ccgagccatc gccagcggca agatcgagga ggccatcgag gcccagcgcc
      421 agttcctcgt gcagcactac ctcacggtcg aggcggccaa ggtcgagacc gaggtgcgcc
      481 gggccgtgga gcgcagcgag cgcaacttcg ggaaggccga cgagcagatc ctcaaggagc
      541 gccggaacct cgactacgtg aacgccggcc gcctcatcat ggggcgcttc ggcatgctcg
      601 gatcggtgcc ggtggagcga gcccgcgccg acatggagca gatgcgcgag ctggacgccg
      661 acggcttcgc caagctggag cggcgcatgg tggacgcgac cagccgggcc aaggcgtggc
      721 gagaggtggc gctcgacgag ttccgcgtgc tggcggccga ccttgacggg ctgtggtcgg
      781 aggcgcaccg ggctgaacag gtccgcatcg acggcaagat gcgcgacatc gccgaagttc
      841 gcgccgagct cgaggaccgc ttggtccgca ccttcggggg caagcggaac gaggatggca
      901 tcgtcgaggc gtgggagaag gtgccgcgct acttgggcgg cgacatcgtg tcctacatga
      961 cgcggctgga ggcgttcacc cgcatggtgg acggcgaggc cggtgggccg tggcaccgct
     1021 acgtctacca gccgctcaag gatgcgacgg tcgcggccga actggagaag gccgaacaca
     1081 aggcgtggat cgagtcgtgg ctccgcgagt ttgggccgct gtccgagaag atcgacacca
     1141 agcggttctt cggggacggc aacgcgctgc ccacgacgct ggccgaggtg gttgggttgc
     1201 tgctgcacct tggaaccgag tcgggcgagc gggccgtggt gctcggcaac gggctcggcc
     1261 accaggtcaa gggcgacttg gtgacccggc tgccggtcat cctccgagag ctggccgacg
     1321 ccgggaagat caccgagaag cacttccagc aggtccagaa gctgtgggac tacatcgcca
     1381 agaacctgcg cgagcgtgtg ttcaagacca cgcgcgaggt gtggggcttc tggcccaagg
     1441 aggtcgaggc caaggtggtg cagacgcctt tcgggacgat ccccggcggc tacttcccgg
     1501 cgaaggtgga ccacgagaag gcgaccgtgc agacggcggc caagctggag gctctgagca
     1561 agggcgggcg cgacgccttc gtggcggcaa acccgagcgt gccgcgtggc atgacgatcc
     1621 agcggcagaa caacgtggta cagccgcgcg tgctcgacct cggcatggtc ggtcaacaca
     1681 tggacgaagc ggtcaacatc atccaccttc agccggcgat cagcgacatg gcgcggctgt
     1741 tcggcggccg gcggaacacg atggcggcga cgctggccca ggtgctgccg aaggcgcagc
     1801 ggaacatcat cggcccggcc ctggagcgca tggcgcgcga cacgatcgag gcgccgacga
     1861 accggacgat ccgaggcatc gtgcggtggg cgcggaaggt tgccagcctc gccttcctcg
     1921 gcttcaaccc gcgcaacgcc gcgcagcagt tcaccggcct gttcaacgcg gtgccggagg
     1981 tcggcgcgtc ccggctgtgg tcggcgatcc gcgaagtggc gttccatcct gggcgcaccg
     2041 tcgagcagat catcggcaag agcgacgcca tgcgcgcccg gtggagcgac gccatgcagt
     2101 tggcggcgca ggacatccag cagatcctga acccgagcac ggcgcgcaag acgcttcagc
     2161 aggcgcagcg ggccgcgttc ttcttgcagc ggctggtgca gcagaagacc gacgccatcg
     2221 tgtggaaggc cgcctacgag aaggccgtcg ccgagctcgg gcctggcatg gacgtcgaca
     2281 agctggacgc ggaggccgtg cgtcgcgccg actcggctgt gcgactgacg cagggagaga
     2341 agacgccgct ggacgtgcca acgttcctga gcggcaacgc ggtcgcacag atgttcacgc
     2401 agttcgccga ctaccccaac acggtgctca accagaacat cgcggcgaag gacgggcagc
     2461 ggttcgcggt gttcgcgtgg accgtgcttg ccccgacgct ggcgagcagt gccatcgcgc
     2521 tgacgttcgc gttcggcaag atcggcagca cgcgcgacca aggcgacccg tacgaggagc
     2581 ggctggccaa gttcatcttc ggcgatcagg tgcgcggcat cgtcgggttg gtcccgatcg
     2641 ccggcccgct ggcgtccagc gcgctcaatg agctgttcgg cctgacgaac gatccgggcg
     2701 gcctgcgcaa ccagcccttc gcgtcgtggt cgatgatcca gaatctcgcc aacgtggcga
     2761 cgggggacgc gaagttctcc gacgctctgg cggcgatcgc cttcgggatc ggggcgccgg
     2821 ctcggccgct ggtcaacgtc acgcgctacc aggagcgcat cgccgcaggg cgcgagccgc
     2881 aaggcgacat cgtggactac ctgcgggtgc tgctcaacgg gcggtagcaa gaccgcggat
     2941 cgcctcgatc ccctcccgca tgtgctccga gagcgcgagg gccgccgcag ggtcgccgcg
     3001 ctgcgccacc gacagcgcct gctcccacgt catcgtggag acgcggcgcg ggcgcatcat
     3061 cgcggtgcac gtccacccgg cgtcaagaag gcatagcgca gcaagcgcaa ggcgaagcgc
     3121 tgttcggagc tgccgacgtc tctccatgcg cggattccaa cacaccgcgc ttgcacttgc
     3181 cagaccctcg cgatatgacc cgcccagcga agccttgagg accgtatgca tcaacaaccg
     3241 gaaacatgcc cgatcggctt gcgccgtttg ggcgatcagc gcagcggaac tctcggcgcc
     3301 atgggcgtgg ccgagttgca tcgggttcct cggcttcgca accagaccgc cgttgcgctg
     3361 actcgccgca ccgacaccct ggctcggtgc ggcaccttta ccttcccctt gagcgtgcgc
     3421 gtcggaacct ccgcgcgtgc tctgcccgtc gcccaaagtt cggtcagcat cggcgacggg
     3481 aacctctctg cctgcgtcgt gcggcatggt gcgccgggag atccggcgac cagaacagac
     3541 gggctgcatg taacaccgcg cgacgcgggc gactccttct tccgctcggc gtgtcgccgg
     3601 gcagccgcga gcccggtggg cgaaagccag ggcggactct caaactggtg gaccagccac
     3661 cggcctgttg cgtccgggat cgcggtgcgc cgtcgcgtag ccctttgccg ggggcgatcg
     3721 gcggtttcct acaccctgcg cgcctcgtct gtagcgacga gactccttcc ccc
//
LOCUS       DAOQNN010000313         3773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16106_length_3773_cov_10.529048, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000313 DAOQNN010000000
VERSION     DAOQNN010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16106_length_3773_cov_10.529048"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..788)
                     /gene="yihA"
                     /locus_tag="PKV97_13590"
     CDS             complement(102..788)
                     /gene="yihA"
                     /locus_tag="PKV97_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNB06960.1"
                     /translation="MPLFRNATFEISIAKPSGLPPPSGAEIAFAGRSNAGKSSAINTL
                     AGHVRLAYVSKTPGRTQLINFFRLNNGALLVDLPGYGYAAVPEKIRRQWSGLIETYLR
                     TRESLIGLVLIMDSRHPLTPLDRNMIDWFAPSGRPMHVLLTKSDKLSRGAAAATLQEV
                     RRALEPLGPQVTVQLFSSLKKSGVEEVERVIGGWLVPAGTVGGDLADTPAGAVDAPPG
                     AQTKAPDQGT"
     gene            912..1541
                     /locus_tag="PKV97_13595"
     CDS             912..1541
                     /locus_tag="PKV97_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB06961.1"
                     /translation="MIKRSLLLSSLLLVAGGLQAQDQAPDLEKAKQTAETVCAACHSA
                     DGNSQLPANPKLAGQHEDYLFKQLREFKGWNGNPPVRENAIMGAMVAGLEEADMRGLA
                     KYFATFEQKPEPAKSLGTIDLGQKIWRAGIPAKGVPACAACHGPAGAGMPAQYPRLSG
                     QFAEYTEAQMKAFRDGVRANDPNGMMRTIALKMTDPEIKAVADYATGLR"
     gene            1668..3752
                     /locus_tag="PKV97_13600"
     CDS             1668..3752
                     /locus_tag="PKV97_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNB06962.1"
                     /translation="MHSGIARALFELFSSMRFAISLLTILSIASVVGTVVRQNEPFNA
                     YLNQFGPFWFPVFEKLGLYSVYNAGWFLVILAFLVLSTTLCIVRQTAPMLREMRSFRE
                     RAREASLRSFAHRVSLVPALPPADAVERAQAYLAHAGFTSRVADNGEGTLLAARQGSA
                     GRIGYFLAHGAIVLICIGGLLDGNLPLRLQVWLGDKHTTAADQVIANIPPSARLGTGN
                     PSFRGDVFIPEGRSTSFAVLGLADGILLQELPFNVTLDKFHIEHYQNGMPKRFASDIR
                     ITDRSSGQTTRHTIEVNHPLTVDGVTLYQSSFEDGGSKLAIKARSLLPGRPGVLREIE
                     GVVGESLAFADGAAGFSYTLEFTDFRAFNVEDMRGNEGGQGDEGEAAPRGMDKLQRHL
                     GSGAKGAEVRDLRNIGPSFTFKLRDPAGQAREFHNYMLPIEQGGRWFLYTGLRDAQAD
                     AFRYMRIPLDERGGVDAWFAIRDRLLDPAQRDALVARFAARSFPAGRGQDAMREALVQ
                     SARQTLGLFAEKGYETLGGFIEKSVPEAERGPAADVLVRILQGLAWEAWMLHREAGGE
                     PPLQLDPARATFVNDTIAAISDSQFYGAPAWLQLDGFEERHATVLQATRSPGMPLVYL
                     GALLLVLGVFAMLYIRERRLFVLVKPNEALVAMSSNRRALDVDEAFRQHVEGLAAMLG
                     APVPASEPREGA"
BASE COUNT          588 a         1388 c         1219 g          578 t
ORIGIN      
        1 catcttagca ggcagatggc gccgtgcccc caggccggcc atctcccgct tcacctccct
       61 gagcgggtgc ctcacgcctg gtgaggcatt ttcgcccccg actacgtccc ctggtcgggg
      121 gcttttgttt gtgcgcccgg cggcgcatcg accgcgccgg caggcgtgtc ggcgaggtcg
      181 ccgcccaccg tgccggctgg caccagccag ccgccgatca cgcgctcgac ctcctccacc
      241 ccgctcttct tcaggctgga aaacagctgc acggtgacct gcggcccgag cggctcgagc
      301 gcgcgacgca cctcctgcag ggtcgccgcg gcggcgccgc gcgagagctt gtcgctcttg
      361 gtgagcagca cgtgcatcgg cctgcccgag ggcgcgaacc aatcgatcat gttacggtcg
      421 agaggcgtga gcggatgtcg gctgtccatg atcagcacga gtccgatcag gctctcgcgg
      481 gtacgcaggt aggtctcgat cagccccgac cactggcggc ggatcttctc gggcaccgcg
      541 gcgtagccgt agccgggcag atcgacgagc agcgcgccgt tgttgagacg gaagaagttg
      601 atcagctgcg tgcgcccggg cgtcttcgac acgtaggcca ggcgcacgtg accggcgagg
      661 gtattgatcg cgctcgactt gccggcgttg gagcgcccgg cgaaggcgat ctccgcgccc
      721 gagggcggtg gcaggcccga aggcttggcg atcgagattt cgaatgttgc gttgcggaac
      781 agaggcatgg agggagatca ctgaaaaccg cgccttgcgc ggcgaacaga cctcatcgca
      841 tcgcgcaatc aggcgttcgt gtattagaat accgcgtttg aaacaatcac tcacggcttt
      901 ccgaggacat catgatcaag cgttccctgc tgctctcgtc gcttctgctg gtcgccggcg
      961 gccttcaggc ccaggaccag gccccggacc tcgaaaaggc caagcagacc gccgagacgg
     1021 tctgcgcggc ctgtcacagc gccgacggca acagccagct cccggccaac cccaagcttg
     1081 ccggccagca cgaggattac ctcttcaagc agctgcgcga gttcaagggc tggaacggca
     1141 atccgccggt gcgtgagaac gcgatcatgg gcgcgatggt cgccggcctc gaggaagccg
     1201 acatgcgcgg cctcgccaag tacttcgcca ccttcgagca gaagcctgag ccggccaaga
     1261 gcctcggcac catcgacctc ggccagaaga tctggcgcgc gggcatcccc gccaagggcg
     1321 tccccgcctg tgccgcctgc cacggccccg ccggcgcggg catgccggcg caatatccgc
     1381 gcctgtccgg ccagttcgcc gaatacaccg aagcgcagat gaaggccttc cgcgacggcg
     1441 tgcgcgcaaa cgacccgaac ggcatgatgc gcaccatcgc gctcaagatg accgatccgg
     1501 agatcaaggc cgtcgccgac tacgcgaccg gcctgcgctg accctgcgcc agccctgcgc
     1561 agcccccacc gccgagccgc gtgggggcgg gcgggggcga tcgtctgcat cgcgcgcccg
     1621 cgccgcacgc cctgcgacgc tggcgtccgt ctttctccga ggtccggatg catagcggca
     1681 tcgcgcgcgc cctcttcgag ctcttcagct cgatgcgttt tgccatctcc ctgctgacga
     1741 tcctgtcgat cgcgtcggta gtgggaacgg tcgttcgcca gaacgaaccc ttcaacgcct
     1801 atctcaacca gttcggtccg ttctggttcc ccgtcttcga gaagctcggc ctgtactccg
     1861 tgtacaacgc cggctggttc ctcgtcatcc tcgccttcct ggtgctgtcg accacgctgt
     1921 gcatcgtgcg ccagaccgcg ccgatgctgc gcgagatgcg cagcttccgc gagcgcgcgc
     1981 gcgaggcctc gctgcgcagc ttcgcccacc gcgtcagcct cgtgcccgcg ctgccccccg
     2041 ccgacgcggt agagcgcgcg caggcctacc tcgcgcacgc cggcttcacc agccgggtcg
     2101 ccgacaacgg cgaaggcacc ctgctcgccg cccgccaggg cagcgccgga cgcatcggct
     2161 acttcctcgc ccacggcgcg atcgtgctga tctgcatcgg cggcctgctc gacggcaacc
     2221 tgccgctgcg cctgcaggtg tggctgggcg acaagcacac caccgcggcc gaccaggtca
     2281 tcgcgaacat tccaccgtcg gcccgtctcg gcacgggcaa cccgagcttc cgcggcgacg
     2341 tcttcatccc cgagggccgc agcaccagct tcgcggtgct cggcctggcc gacggcatcc
     2401 tgctgcagga actgcccttc aacgtcacgc tcgacaagtt ccacatcgag cactaccaga
     2461 acggcatgcc caagcgcttc gccagcgaca tccgcatcac cgaccggtcg agcggccaga
     2521 ccacgcggca caccatcgag gtcaaccacc cgctcaccgt cgacggcgtc accctctacc
     2581 agtcgagctt cgaggatggc ggctcgaagc tggcgatcaa ggcgcgcagc ctgctgcctg
     2641 gccgtcccgg ggtgctgcgc gagatcgagg gcgtggtggg cgaaagcctg gcgttcgccg
     2701 acggcgccgc gggcttctcc tatacgctgg aattcaccga cttccgggcc ttcaacgtcg
     2761 aggacatgcg cggcaacgag ggcgggcaag gcgacgaagg cgaggccgcg ccgcgcggca
     2821 tggacaagct gcagcgccac ctcggcagcg gcgccaaagg cgcggaggtg cgcgacctgc
     2881 gcaacatcgg ccccagcttc accttcaagc tgcgcgaccc cgccggccag gcgcgcgagt
     2941 tccacaacta catgctgccg atcgagcagg gcgggcgctg gttcctctac accggcctgc
     3001 gcgacgcgca ggccgacgcc ttccgctaca tgcgcatccc cctcgacgag cgcggcggag
     3061 tggatgcctg gttcgcgatc cgcgaccgcc tgctcgaccc cgcgcagcgc gacgctctgg
     3121 tcgcccgctt cgccgcgcgc agcttccccg ccggtcgcgg ccaggacgcg atgcgcgagg
     3181 ccctggtgca gtcggcccgg cagaccctcg ggctgttcgc cgagaagggc tacgagacac
     3241 tcggcggctt catcgagaag tccgtgcccg aggccgaacg cggcccggcc gccgacgtgc
     3301 tggtgcgcat cctgcagggg ctcgcctggg aggcatggat gctgcaccgc gaggccggtg
     3361 gcgaaccgcc gctgcagctc gacccggcgc gcgccacctt cgtcaacgac accatcgcgg
     3421 cgatctccga cagccagttc tacggcgcgc ctgcctggct tcagctcgac ggcttcgagg
     3481 agcgtcacgc caccgtgctg caggcgacgc gctcgccggg catgccgctc gtctatctgg
     3541 gcgcgctgct gttggtgctg ggcgtgttcg cgatgctcta catccgcgaa cgtcgcctgt
     3601 tcgtgctggt gaagccgaac gaggcgctgg tcgcgatgtc ttccaaccgc agggcgctcg
     3661 acgtcgacga ggccttccgc caacacgtcg aaggcctcgc cgccatgctc ggcgcccccg
     3721 tgcccgcatc cgaaccccga gaaggtgcat gatggaaatc gcccgcccct ccc
//
LOCUS       DAOQNN010000314         3766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16149_length_3766_cov_8.159256, whole genome shotgun sequence.
ACCESSION   DAOQNN010000314 DAOQNN010000000
VERSION     DAOQNN010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16149_length_3766_cov_8.159256"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /gene="lexA"
                     /locus_tag="PKV97_13605"
     CDS             complement(<1..385)
                     /gene="lexA"
                     /locus_tag="PKV97_13605"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNB06963.1"
                     /translation="MTARSENLTARQQEILDFIRSTVESEGRPPTRAEVCTAFGFRSP
                     NAAETHLRALAAKGAILLEEGRARGIRLAEALGLPLVGRVAAGSPILAAEHVEARYQL
                     DPALFSPRADYLLRVRGMSMRDAGIL"
     gene            complement(445..1482)
                     /locus_tag="PKV97_13610"
     CDS             complement(445..1482)
                     /locus_tag="PKV97_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNB06964.1"
                     /translation="MPRPHPNAPAKPPAGMRAMQLERPGEALRAVHLAVPRPGPGQVL
                     LRVEACGVCRTDLHLIDGELPDPVLPMIPGHEIVGRVVELGEGVGDAAGMVPGQRIGV
                     PWLGWTCGHCGFCASQRENLCDAARFTGYQIQGGYAEYTVADARYCFPIDEALDAAEA
                     APLLCAGLIGYRALEMAGAARRIGLYGFGAAAHIITQVAVWQGREVYAFTRHGDSASQ
                     AFARRLGACWTGAGEERPPHPLDAALIFAPSGELVPAALRAVAKGGTVVCAGIHMSTI
                     PAFPYDILWGERRIVSVANLTRRDGDEFLALAPRVPVRTEVVRFALEDANEALERLRR
                     GALQGAAVLLP"
     gene            1703..2200
                     /locus_tag="PKV97_13615"
     CDS             1703..2200
                     /locus_tag="PKV97_13615"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNB06965.1"
                     /translation="MLQSGDIAPAFSLPDADMEIFDLASVAGKHNVVLYFYPRDKTPG
                     CTRQAADFSDHDDEFARYDCIVVGVSPDDCLTHAEFRDQEGLSIRLLSDSDTEVCRLY
                     NVWHEREVDGMKKMGVRRSTFVIDKQGRIRHALYDVTPRGHAAAVFELVKELEPQHAK
                     RNRQE"
     gene            2175..2663
                     /locus_tag="PKV97_13620"
     CDS             2175..2663
                     /locus_tag="PKV97_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB06966.1"
                     /translation="MQNEIVKNSVVTLKYTVRDPEGAVIDDGEHPLVYLHGGYDGIFP
                     LLEEALHGKKVGEQLQVKLQPEDAFGDYDEELILVEDAKLFPDNIEIGMSFERVSEDG
                     EEDMIYRITDIADGKVVVDGNHPLAGVALVFDVTVAEVRAASAEEITHGHVHGPGGHH
                     HH"
     gene            2835..>3766
                     /locus_tag="PKV97_13625"
     CDS             2835..>3766
                     /locus_tag="PKV97_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB06967.1"
                     /translation="MNAPLPADLPDAATAALLHVEGLGVAIARTDGEEVRPVDGVNFA
                     IAAGETFALLGESGCGKSMTALALARLLPDGGRIAAGSVRLGDEDLLALPEAAMRRVR
                     GGRIGMIFQEPATSLNPVMSVGAQIAEALAAHGLRGAAARAEACRLLAAVGIPDPERR
                     LQEFPFQFSGGMKQRVMIAIALAGRPRLLIADEPTTALDVTIQAQVLDLLAELQRREG
                     MAMLLITHDLGVVARVAHRVGVMYAGQVVESGPREAFFATPLHPYSRKLFAALPEAAK
                     RGGALDAPVGSVPPPDRHFPGCRFAERCPAAFDR"
BASE COUNT          567 a         1287 c         1361 g          551 t
ORIGIN      
        1 cgaggatgcc ggcgtcgcgc atgctcatgc cgcgcacgcg cagcaggtag tcggccctgg
       61 gcgagaacag cgcggggtcg agctgatagc gcgcctcgac gtgttcggcg gcgaggatgg
      121 ggctgcccgc ggcgacgcgg ccgaccaggg gcaggccgag tgcctcggcc aagcggatgc
      181 cgcgcgcgcg cccctcctcg agcaggatcg cgcccttggc ggcgagcgcg cgcaggtggg
      241 tctcggcggc gttgggcgag cggaagccga aggcggtgca cacctcggcg cgggtgggcg
      301 ggcggccttc ggactcgacg gtggagcgga tgaaatcgag gatttcctgc tggcgcgcgg
      361 tgaggttttc gctacgggcg gtcatgacag cctccatggg atggctgtaa ttttaaacag
      421 tcccgcagcg ctggcaagcg gaattcaggg cagcagcacc gccgccccct gcagcgcgcc
      481 gcggcgcagg cgctcgagcg cctcgttggc gtcctcgagg gcgaagcgga cgacctcggt
      541 gcgtaccggc accctgggcg cgagggcgag gaattcgtcg ccgtcgcggc gggtgaggtt
      601 ggcgaccgag acgatgcggc gctcgcccca caggatgtcg taggggaagg cggggatggt
      661 gctcatgtgg atgcccgcgc acaccaccgt gccacccttg gcgacggcgc gcagcgcggc
      721 ggggacgagc tcgccggagg gggcgaagat cagcgcggcg tcgagcgggt gcggggggcg
      781 ctcttcgccc gcgcccgtcc agcaggcgcc gagccggcgg gcgaaggcct ggcttgcgct
      841 gtcgccgtga cgggtgaagg cgtacacctc gcgaccctgc cacaccgcca cctgggtgat
      901 gatgtgggcg gccgcgccga agccgtagag gccgatgcgg cgcgcggcgc ccgccatctc
      961 cagcgcacga tagccgatca ggccggcgca cagcagcggc gcggcctcgg cggcgtcgag
     1021 cgcctcgtcg atcgggaagc agtagcgtgc gtcggccacg gtgtattcgg cataaccgcc
     1081 ctggatctgg tagccggtga agcgcgccgc gtcgcagagg ttctcgcgct ggctggcgca
     1141 gaagccgcaa tgcccgcagg tccaccccag ccagggcacg ccgatgcgct gacccggcac
     1201 catgcccgcg gcgtcgccga cgccttcgcc cagctcgacc acgcggccga cgatctcgtg
     1261 gccgggaatc atcggcagca ccgggtcggg cagctcgccg tcgatgaggt ggagatcggt
     1321 gcggcacacg ccgcaggcct ccacccgcag cagcacctgc ccggggccgg ggcgcggcac
     1381 cgccagatgc accgcgcgca gggcctcgcc ggggcgttcg agttgcatcg cgcgcatgcc
     1441 ggcagggggc ttggcggggg cgttcggatg gggccggggc atgcttgcgg tctccaggac
     1501 aggcggaacg atggtgcgct gcgacttgag tacggccccg atctgggcta ttcgttggac
     1561 acggagccgc atcgcggcac ccgacacgac gacaaggaaa agggaggaga cccccatgct
     1621 cgaccagtcc gaagcctgcg gtcccggcgg tagtgttcgc ctggggtctc ggctagaatc
     1681 cggcccacat cagggagaac acatgctgca gagcggagac atcgcccccg cgttttcgtt
     1741 gccggatgcc gacatggaaa ttttcgacct cgcgtcggtg gccggcaagc acaatgtggt
     1801 tctctacttt taccctcgcg acaagacccc gggctgcacg cgtcaggccg ccgatttcag
     1861 cgaccacgac gacgagttcg cccgctacga ctgcatcgtg gtcggcgtga gcccggacga
     1921 ttgcctgacc cacgccgagt ttcgcgacca ggaaggattg tcgatccgcc tgctctccga
     1981 ttcggacacc gaggtgtgcc ggctctacaa cgtatggcat gagcgcgaag tcgacgggat
     2041 gaagaagatg ggcgtgcggc ggtcgacctt cgtgatcgac aagcagggca ggatccgcca
     2101 cgcgctctac gatgtgacac cccgcggcca cgccgccgcg gttttcgaat tggtcaagga
     2161 actggaaccc cagcatgcaa aacgaaatcg tcaagaatag cgtggtgacg ctgaagtaca
     2221 ccgtgcgcga ccccgaaggc gcggtgatcg acgacggcga gcacccgctc gtttatctgc
     2281 acggcggcta cgatggcatc tttccgctgc tcgaagaggc cctgcacggc aagaaggtcg
     2341 gcgagcagct gcaggtcaag ctgcagcccg aagacgcctt cggcgactac gacgaggagc
     2401 tgatcctggt cgaggacgcc aagcttttcc cggacaacat cgagatcggc atgagcttcg
     2461 agcgcgtctc cgaggacggc gaggaagaca tgatctaccg catcaccgac atcgccgacg
     2521 gcaaggtcgt ggtcgatggc aaccacccgc tggccggcgt ggcgctggtg ttcgacgtca
     2581 ccgtcgccga ggtgcgcgcg gcgagcgccg aggagatcac ccacggccac gtgcatggtc
     2641 ccggtggtca tcaccaccac tgattgcacc gatgtcagga cggggccgcg caagcggccc
     2701 tttcgacatg tggacggcac ctgcggctgc gtggtgcgtg tcaggggccg tccgcctcgt
     2761 ccgcgcctcg ttcgcctctc tccgccttcc tccgcctcga tctgctgcgg ccctgcaagg
     2821 gtcgtcccac gcgcatgaac gctccgctcc ccgccgatct ccctgacgcc gcgaccgctg
     2881 cgctgctgca cgtcgaagga ctgggcgtgg cgatcgcgcg caccgacggc gaggaggtcc
     2941 gcccggtcga cggggtgaat ttcgccatcg ccgcgggcga gaccttcgcc ttgctcggcg
     3001 aatcgggctg cggcaagtcg atgaccgcgc tcgcgctcgc ccgcctgctg cccgacggcg
     3061 gccgcatcgc cgcgggcagc gtgcgcctgg gcgacgagga cctgctcgcg ctgcccgagg
     3121 cggcgatgcg gcgggtgcgt ggcgggcgca tcggcatgat cttccaggag cccgccacca
     3181 gcctcaaccc ggtgatgagc gtgggtgcgc agatcgccga ggcgctcgcc gcccacggct
     3241 tgcgcggcgc ggcggcgcgc gccgaagcct gtcggctgct cgcagcggtg ggcatccccg
     3301 accccgagcg ccggctgcag gagtttccct tccagttctc gggcgggatg aagcagcgcg
     3361 tgatgatcgc gatcgcgctc gccggccgtc cgcgcctgct gatcgccgac gagccgacca
     3421 ccgccctcga cgtcaccatc caggcccagg tgctcgacct gctcgccgag ttgcagcgcc
     3481 gcgagggcat ggcgatgctg ctgatcaccc acgatctcgg cgtggtcgcg cgcgtcgccc
     3541 accgcgtggg ggtgatgtat gccggtcagg tggtggagag cggtccgcgc gaggccttct
     3601 tcgccacgcc gctgcatccg tattcgcgca agctcttcgc cgcgctgccc gaggcggcca
     3661 agcgcggcgg cgcgctcgac gcgccggtgg gcagcgtgcc gccgcccgat cggcattttc
     3721 ccggctgccg cttcgccgag cgctgcccgg cggccttcga ccgctg
//
LOCUS       DAOQNN010000315         3755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16229_length_3755_cov_9.475676, whole genome shotgun sequence.
ACCESSION   DAOQNN010000315 DAOQNN010000000
VERSION     DAOQNN010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16229_length_3755_cov_9.475676"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..600)
                     /gene="hslV"
                     /locus_tag="PKV97_13630"
     CDS             complement(64..600)
                     /gene="hslV"
                     /locus_tag="PKV97_13630"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNB06968.1"
                     /translation="MEQYRGTTILSVRRGNRVALGGDGQVTLGNIVIKASARKVRTLY
                     NGQILAGFAGGTADAFTLFERFEAKLDKHQGNLLRSAVELAKDWRTDRMLRRLEAMLA
                     VADREHSLVITGNGDVLEPEQGIVAIGSGGAYAQSAARALLENTELEPREIVSKALSI
                     AGDLCIYTNHHHTIEVLD"
     gene            complement(644..1825)
                     /locus_tag="PKV97_13635"
     CDS             complement(644..1825)
                     /locus_tag="PKV97_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter"
                     /protein_id="HNB06969.1"
                     /translation="MALPFFGKKPVSPAPGAPRDARAGPGADALRDDRPPPTELSALD
                     FTGTDHGRALARVAGQVEVRECGSGIGAAFEEAAVLYANGSDDDALAVLEAAVGAEGG
                     EGGEGAWLMLLDLYRLRGERQRFESRVLDYATRFERSPPPWEDLSRVAGKAAGTELPL
                     VNLSGQLSAAAERQFAQLAKIARRSGGVRIDVAKVRGVDDAGCAVWRGLLAGLAAERL
                     QVRLVNVRALADILSQAVCPGTARARDTWLMLLELLQYAGDQARFEDVAVEYAVTFEE
                     SPPSWEGRGDAPRAPAAQGDEAPPADPEAFAFEGELCGAANDTLRRLAAFAVERREVR
                     IDCRRLRRLDFVCAGTLFNILATLQAQGKLVCLRDVNAMVAGLLRVMSVDKVAHVTLR
                     H"
     gene            1984..2436
                     /gene="ybgC"
                     /locus_tag="PKV97_13640"
     CDS             1984..2436
                     /gene="ybgC"
                     /locus_tag="PKV97_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HNB06970.1"
                     /translation="MQPKTVSDAGGESLPASSRFSLSVRVYYEDTDAGGVVYYANYLK
                     FCERTRTEWLRALGVAQHALLDEQGLGFVVRSVQADYLAPARLDDALAVSARIASRRR
                     ASLVFEHEIRRGGELLFTAQVLVACVDLRRMKPAALPASLHALLESPA"
     gene            2433..3101
                     /gene="tolQ"
                     /locus_tag="PKV97_13645"
     CDS             2433..3101
                     /gene="tolQ"
                     /locus_tag="PKV97_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HNB06971.1"
                     /translation="MTVTHDLSILSLISQASVLVQLVMALLASLSLVSWYWIFRKAFQ
                     IRQARAQTDEFERDFWSGGDLNTLYESAGRHQSEGMERIFEAGYREFTKLRGKGHDHG
                     ATLDGARRAMRATYQREIDDLEAHLAFLASVGSVSPYIGLLGTVWGIMNSFRGLSSVG
                     AATLAQVAPGIAEALVATAIGLFAAIPAVVAYNRFAHDIDRLGIRFESFMEEFSNILQ
                     RNLR"
     gene            3121..3537
                     /gene="tolR"
                     /locus_tag="PKV97_13650"
     CDS             3121..3537
                     /gene="tolR"
                     /locus_tag="PKV97_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HNB06972.1"
                     /translation="MRQRRLMNQINVVPYIDVMLVLLVIFMVTAPMIQQGTINVPSAG
                     AGTPPPQSEAIFIEVKADGAYALRASNSGSSRPVRRDQLSTALKDALAKNPEQPFLVA
                     ADSKLDYQKVIDVLETARDAGVRKISLVTQSATSQK"
     gene            3534..>3755
                     /locus_tag="PKV97_13655"
     CDS             3534..>3755
                     /locus_tag="PKV97_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HNB06973.1"
                     /translation="MRDRPAPHRREPPGKWQSLGLTVAVHLGLFLFLFFGIRWQSPPP
                     AALEVGLASAPERSAPAAARPQPAPEPRPE"
BASE COUNT          603 a         1361 c         1215 g          576 t
ORIGIN      
        1 acgatgtgct tgtcgagttc ggagacgatc tccggcgggg tcatctgggt catgcggtca
       61 gcctcaatcc agcacttcga tggtgtggtg gtgattggtg tagatgcaca ggtcgccggc
      121 gatggaaagc gccttggaca cgatttctct gggctcgagc tcggtgttct cgagcagtgc
      181 gcgcgcggcc gactgcgcgt aggcgccgcc gctgccgatg gccacgatgc cctgctcggg
      241 ctcgagcacg tcgccgttgc cggtgatcac cagcgaatgc tcgcgatcgg ccaccgcaag
      301 catggcctcc agccggcgca gcatgcggtc ggtgcgccag tccttggcga gctcgaccgc
      361 gctgcgcaac aggttgccct ggtgcttgtc gagcttggcc tcgaaacgct cgaacaaggt
      421 gaaggcgtcg gcggttccac cggcgaaacc ggcgaggatc tgaccgttgt agagcgtgcg
      481 caccttgcgc gcggaggcct tgatgacgat gttgccaagg gtgacctggc cgtcgccgcc
      541 gagcgcgacg cggttgccgc gccgcaccga caggatggtg gtgccgcgat actgttccat
      601 gaaggattct ccgtccccgc ggaaatcggg gctggtgtgt tgttcaatga cgcagggtga
      661 cgtgagccac cttgtcgacg ctcatgaccc gcagcaggcc ggcgaccatg gcgttcacgt
      721 cgcgaagaca gacgagttta ccttgagcct gcaacgtggc gagaatattg aagagcgtgc
      781 cggcgcagac aaaatcgagc cggcgcaggc gccggcaatc gatccggacc tcgcggcgct
      841 cgaccgcgaa ggcggcgagg cgccggaggg tatcgttggc agccccgcag agctcgccct
      901 cgaacgcgaa cgcctccgga tcagccggcg gcgcctcgtc cccctgcgcc gcgggcgccc
      961 ggggtgcgtc gccgcgacct tcccaggacg gcggcgactc ctcgaaggtc accgcatact
     1021 cgaccgcgac atcctcgaaa cgtgcctggt cgccggcgta ctgcaacagt tcgagcagca
     1081 tcagccaggt gtcgcgcgcc cgcgccgtcc cggggcacac ggcctgcgac aggatgtcgg
     1141 cgagcgcacg cacattgacc agcctcacct ggagccgctc cgcggccagc ccggcaagca
     1201 ggccgcgcca gaccgcgcag cccgcatcgt ccaccccgcg caccttggcg acgtcgatgc
     1261 gcaccccgcc gctgcggcgc gcgatcttgg cgagctgggc gaactggcgc tcggccgctg
     1321 ccgagagctg cccgctcagg ttgaccagcg gcagctcggt gcccgccgcc ttgcccgcca
     1381 cccgcgacag atcctcccag ggcggcggcg agcgctcgaa gcgggtagcg taatcgagca
     1441 cgcgcgactc gaagcgctgg cgctccccgc gcaggcggta gagatcgagc aacatcagcc
     1501 aggcaccttc accgccttcg ccaccttccg cgccgacggc cgcctcgagc acggcgagcg
     1561 cgtcgtcgtc gctaccgttc gcatacagca ccgcagcctc ctcgaaggcc gcgccgatcc
     1621 cgcttccgca ctcgcgcacc tcgacctgcc cggccacgcg tgcgagcgcg cgcccgtggt
     1681 cggtgccggt gaagtcgagc gcggacaact cggtaggcgg gggacgatcg tcgcgcagcg
     1741 cgtcggctcc cggcccggct cgcgcgtcgc gcggtgcgcc gggcgcgggc gaaacgggtt
     1801 tcttgccgaa gaaggggagc gccacgtccg tattccgatg tcgcggagcc ccatgcgagc
     1861 cgccgcaacc aacgacattc acagaaaagc gcttttgcaa caagcaataa gcgcaacaaa
     1921 aatccatcga tcaagaacgc gacagacgtg cattcggtgc cgtggctcgg ctagaatgct
     1981 gcaatgcaac cgaaaaccgt ttccgacgcg ggcggcgaat cgctccccgc gtcgtcgcgt
     2041 ttttccctgt ccgtccgagt ctattacgaa gacaccgacg cagggggtgt cgtctattac
     2101 gcaaactatc tcaaattctg cgagcgcacg cgcaccgaat ggctgcgtgc gctcggggtc
     2161 gcccagcacg ccttgctcga cgagcagggc ctgggtttcg tggtgcgctc ggtgcaggcc
     2221 gactacctcg ctccggcacg cctggacgac gcgctcgcgg tgagcgcccg catcgccagc
     2281 cggcgccgcg cgagtctcgt cttcgagcac gagatccgcc gcggcggcga acttctcttc
     2341 accgcgcagg tcctcgtggc ttgcgtggac ctgcggcgca tgaagcccgc agccctcccc
     2401 gcctccctgc acgcccttct cgaaagccca gcatgaccgt cactcacgac ctctccatcc
     2461 tcagcctgat ctcccaggcg agcgtcctcg tccagctcgt catggcgctg ctggcgagcc
     2521 tgtcgctcgt ctcctggtac tggatcttcc gcaaggcctt ccagatccgc caggcgcgcg
     2581 cgcagaccga cgagttcgag cgcgacttct ggagtggcgg cgacctcaac acgctctacg
     2641 agtcggccgg ccgccaccag tccgaaggca tggagcgcat cttcgaggcc ggctaccgcg
     2701 aattcaccaa gctgcgcggc aaaggccacg accacggcgc cacgctcgac ggcgcccgcc
     2761 gcgcgatgcg cgccacctac cagcgcgaga tcgacgacct cgaggcccac ctcgccttcc
     2821 tcgcctcggt ggggtcggtg tcgccttaca tcggcctgct cggcaccgtg tgggggatca
     2881 tgaactcctt ccgcggcctc tccagcgtcg gcgccgccac cctcgcgcag gtcgcgcccg
     2941 gcatcgccga agccctggtc gccacggcga tcggcctgtt cgcggcgatc ccggcggtgg
     3001 tggcctacaa ccgcttcgcg cacgacatcg accgcctcgg catccgcttc gagagcttca
     3061 tggaagagtt ctccaacatc ctgcaacgca acctgcgctg acccggacgg agttccaacg
     3121 atgcgccaac gccgtctcat gaaccagatc aacgtcgtgc cctacattga cgtgatgctg
     3181 gtgctgctgg tgatcttcat ggtcaccgcg ccgatgatcc agcagggcac gatcaacgtg
     3241 ccctccgccg gcgccggcac gccgccgcct cagtccgagg ccatcttcat cgaggtcaag
     3301 gccgacgggg cctatgcgct gcgcgccagc aacagcggga gctcgcgccc ggtgcgccgc
     3361 gaccagctgt cgaccgcgct caaggacgcc ctcgcgaaga accccgagca gcccttcctg
     3421 gtcgccgccg acagcaagct cgactaccag aaggtgatcg acgtgctcga gaccgcgcgc
     3481 gacgccggag tgcgcaagat cagcctggtc acccagagcg cgacttcgca gaaatgaggg
     3541 accgccccgc accgcaccgc cgcgagccgc ctggcaagtg gcagtccctc gggttgaccg
     3601 tggcggtcca cctgggattg ttcctgttcc tgttcttcgg catccgctgg cagagcccgc
     3661 cgcccgcggc gctcgaggtc gggctggctt cggcaccgga gcgcagcgcg ccagccgcag
     3721 cgcgcccgca gccggcgccg gagccccggc cggag
//
LOCUS       DAOQNN010000316         3737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16359_length_3737_cov_11.604291, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000316 DAOQNN010000000
VERSION     DAOQNN010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16359_length_3737_cov_11.604291"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="PKV97_13660"
     CDS             <1..147
                     /locus_tag="PKV97_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNB06974.1"
                     /translation="IGERMDRGRRALLKEGRNEVGPYQSPFEDGMQHFHEFYRRIMEP
                     HIGG"
     gene            complement(343..1281)
                     /gene="ispH"
                     /locus_tag="PKV97_13665"
                     /gene_synonym="lytB"
     CDS             complement(343..1281)
                     /gene="ispH"
                     /locus_tag="PKV97_13665"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNB06975.1"
                     /translation="MNDKEILLANPRGFCAGVERAIEIVERALKRYGAPIYVRHEVVH
                     NKFVVDDLRAKGAIFVDELDEVPTGNTVIFSAHGVSLAVREEAERRGLRVFDATCPLV
                     TKVHVEVARMRDQGKEIVMIGHKGHPEVEGTMGQVDGGIHLVETPADVAGLQVADPEQ
                     LAYVTQTTLSVDDAAAIVDALRTRFPRVVGPRKDDICYATQNRQDAVKFMTPYADVVF
                     VVGSKNSSNSNRLREVAALRGVPAYLIDNAAGIDPAWIEGKRRIGVTAGASAPEVLVA
                     EVIARLEELSGGAVRNLDGVPERVTFALPKELQDPR"
     gene            complement(1314..1742)
                     /locus_tag="PKV97_13670"
     CDS             complement(1314..1742)
                     /locus_tag="PKV97_13670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNB06976.1"
                     /translation="MSEAIQANSLVTLHYRISLPSGQPLISTFNATPATLQLGAGEML
                     PAMERLLIGLMPGTHHIFELAADEAFGPYRDELIERVKREHMPDEEIEAMSIMEFTAP
                     DGSRYSGLVREIDAQSALIDFNHPLAGKAIRFEVQVIGIS"
     gene            complement(1739..2227)
                     /gene="lspA"
                     /locus_tag="PKV97_13675"
     CDS             complement(1739..2227)
                     /gene="lspA"
                     /locus_tag="PKV97_13675"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNB06977.1"
                     /translation="MLPWLILAVVVGVLDQVTKQMVMANLRLGELIPITGFFDLVLVF
                     NTGAAFSFLADHSGWQRWFFTILAFGISAWLLTLMHHHRHEKLLPTAFALIIGGALGN
                     VYDRLVHGAVVDFLHFHYAGWSWPAFNLADSAITVGVAMMLWGQFFGPKAGDPPRPGA
                     PS"
     gene            complement(2289..>3737)
                     /locus_tag="PKV97_13680"
     CDS             complement(2289..>3737)
                     /locus_tag="PKV97_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB06978.1"
                     /translation="LHAMIANRPDWCISRQRNWGVPIPFFLHKETGELHPRTVELMEE
                     VALRVEQEGIEAWFKLDAAKLLGAEAAQYEKISDTLDVWFDSGTTHKHVLRGSHNDGH
                     PEGPRADMYLEGSDQHRGWFHSSLLTGCAIDGHAPYRSLLTHGFAVDGAGRKMSKSLG
                     NVVVPQEVTGKLGAEILRLWVASTDYSGELSISKEILDRVVEVYRRVRNTLRFLLANT
                     ADFDIATDAVPLEQWLDIDRYALAFTRQLAQQAEADYAKMEFHRVVQALQVFCAEDLG
                     AFYLDILKDRLYTTAAGSLPRRAAQTALWHITQTLLKLMAPILSFTAEEAWTVIHPGK
                     DEGTQDSVMLHTFHALPAQEGEAGLIARWETIRAVRAEGLKVIEALRTEGKVGASLQA
                     ELELALTADKFAAMASLGEDLRFVTMTSAATLSEVARAEDERVVATPSAAQKCERCWH
                     YVDTVGSDAEHPTLCGRCASNLFGEGETRAHA"
BASE COUNT          577 a         1216 c         1319 g          625 t
ORIGIN      
        1 atcggcgagc gcatggatcg cggccgcagg gcgctgctga aggaggggcg caacgaggtc
       61 ggcccctacc agtcgccctt cgaggacggc atgcagcatt tccacgagtt ctaccggcgc
      121 atcatggagc cgcacatcgg cgggtgaagc ggagagaagc gaggcgcgac ctgcggctcg
      181 cgcattccat cacgcgcggc gccgattcaa ggcgccacga tcgcgacttg cgtcgctccc
      241 acaggaaagt acacgcgact cgaccgccaa gtacacgcga ctcgaccgcc gccttgcaga
      301 agcgacgcaa ggcgccattc tctcccgcct tgctccgaag cttcagcgcg ggtcctgcaa
      361 ctccttgggc agcgcgaagg tcacgcgctc gggcacgccg tcgaggttgc gcaccgcgcc
      421 gccgctcagt tcttccaggc gcgcgatcac ctcggccacc agcacctcgg gcgccgaggc
      481 ccccgcggtc acgccgatgc ggcgcttgcc ctcgatccag gcggggtcga tgccggcggc
      541 gttgtcgatc aggtaggcgg gcacgccgcg cagcgcggcg acctcgcgca ggcggttgga
      601 attggagctg ttcttcgacc caaccacgaa caccacgtcc gcgtacgggg tcatgaactt
      661 caccgcgtcc tggcggttct gcgtggcgta gcagatatcg tccttcctcg ggccgacgac
      721 ccgggggaag cgggtgcgca gggcatcgac gatcgctgcc gcgtcatcca cggacagcgt
      781 ggtctgggtc acgtaggcga gctgttctgg gtcggccacc tgcaggccgg cgacgtcggc
      841 cggggtctcg accagatgga tgccgccgtc gacctggccc atcgtgccct cgacctcggg
      901 gtggcccttg tgaccgatca tgacgatctc cttgccctgg tcgcgcatgc gcgccacctc
      961 gacatggacc ttcgtcacca gcgggcaggt ggcgtcgaag acgcgcagcc cgcggcgctc
     1021 ggcttcctcg cgcaccgcga gcgagacgcc atgggccgag aagatcaccg tgttgccggt
     1081 agggacctcg tcgagttcgt cgacgaaaat cgcgcccttg gcgcgcaggt cgtccacgac
     1141 gaacttgttg tgcacgacct cgtggcgcac gtagatcggc gcgccgtagc gcttcagggc
     1201 gcgttcgacg atctcgatcg cgcgctcgac cccggcgcag aagccgcgcg ggttggcgag
     1261 caggatttcc ttgtcgttca tctttcgtct ctgtggggtg tcgcgggcgg cgctcaggag
     1321 atcccgatca cctgcacctc gaagcggatc gccttgcccg ccagcgggtg gttgaagtcg
     1381 atcagcgccg actgcgcgtc gatctcgcgc accaggccgg agtagcgtga gccatcgggt
     1441 gcggtgaact ccatgatgct catcgcttcg atctcctcgt cgggcatgtg ttcgcgcttg
     1501 acgcgctcga tcagctcgtc gcgatagggg ccgaaggctt cgtctgccgc cagttcgaag
     1561 atgtggtggg tgcccggcat caggccgatc agcaggcgct ccatcgccgg cagcatttcg
     1621 ccggcgccca attgcagcgt ggcgggggtg gcgttgaagg tgctgatcag cggctggccg
     1681 ctcggcagcg agatgcggta gtgcagggtg acgaggctgt tggcctggat cgcttcgctc
     1741 aagatggtgc tcccggtcgg ggcgggtcgc cggctttggg accaaagaac tggccccaga
     1801 gcatcatcgc gacacccacg gtgatggccg agtcggccag gttgaacgcc ggccagctcc
     1861 agccggcgta gtggaagtgc aggaagtcga ccaccgcgcc atgcacgagg cggtcataga
     1921 cgttgccgag cgcaccgccg atgatcaggg cgaaggcggt cggcagcagc ttttcgtggc
     1981 gatggtggtg catcagcgtc agcagccagg cgctgatgcc gaaggccagt atggtgaaaa
     2041 accagcgttg ccagccgctg tggtcggcga gaaagctgaa cgcggcgccc gtgttgaaca
     2101 ccagcaccag atcgaagaag cctgtgatcg gaatcagctc gccgaggcgc agattcgcca
     2161 tcaccatctg cttggtgacc tggtcgagca cgccgaccac cacggcgagg atcagccagg
     2221 gcagcatcag agacagcgca ctgcgcgcgt gcggcgtcgg gtgaggtgtc gtcatcgtgc
     2281 gggctgcctc aggcatgggc gcgggtttcg ccttcgccga acaggttgct cgcgcagcgc
     2341 ccgcacaggg tggggtgctc ggcgtcgctg ccgaccgtgt cgacgtagtg ccagcagcgc
     2401 tcgcacttct gcgccgcgct cggggtggcg acgacgcgct cgtcctcggc gcgggccacc
     2461 tcggacaggg tggccgccga ggtcatggtg acgaagcgca ggtcctcgcc caggctggcc
     2521 atcgcggcga acttgtcggc ggtgagcgcg agctccagtt ccgcctgcag cgaggcgccg
     2581 accttgcctt cggtgcgcag cgcctcgatg accttgaggc cttctgcacg cacggcgcgg
     2641 atggtctccc agcgcgcgat cagcccggct tcgccttcct gtgcgggaag ggcgtggaag
     2701 gtgtgcagca tcacgctatc ctgcgtaccc tcgtccttgc cggggtggat caccgtccag
     2761 gcctcctcgg cggtgaagga caggatcggc gccatcagtt tcagcagcgt ctgcgtgatg
     2821 tgccacagcg cggtttgcgc ggcgcgacgc ggcaggctgc cggcggcggt ggtgtacagg
     2881 cgatccttca ggatgtcgag gtagaaggcg cccaggtcct cggcgcagaa cacctgcagc
     2941 gcctgcacga cgcgatggaa ctccatcttc gcgtagtcgg cctcggcctg ctgcgcgagc
     3001 tggcgggtga aggcgagcgc gtagcggtcg atgtccagcc actgttcgag cggcaccgcg
     3061 tccgtggcga tgtcgaagtc ggcggtgttg gcgagcagga agcgcagggt gttgcgcacg
     3121 cggcgataga cctcgaccac gcggtcgagg atttccttcg agatcgacag ctcgccggaa
     3181 tagtcggtgg acgcgaccca caggcgcagg atctcggcgc cgagcttgcc ggtgacctcc
     3241 tgcggcacca ccacgttgcc gagcgacttg ctcatcttgc ggccggcccc gtccaccgcg
     3301 aagccgtggg tgagcaggct gcggtagggg gcgtggccgt cgatcgcgca gccggtgagc
     3361 agcgaggagt ggaaccagcc gcggtgctgg tcggagccct ccaggtacat gtcggcacgc
     3421 gggccttcgg ggtggccgtc gttgtgcgag ccgcgcagca cgtgcttgtg cgtggtgccg
     3481 gagtcgaacc agacgtcgag cgtgtcgctg atcttctcgt actgcgcggc ctcggcaccg
     3541 agcagcttgg cggcatcgag cttgaaccag gcctcgatgc cctcctgctc cacgcgcagc
     3601 gcgacctcct ccatcagctc gacggtgcgc ggatgcaact cgccggtctc cttgtgcagg
     3661 aagaagggga tcggtacgcc ccagttgcgc tggcgcgaga tgcaccagtc ggggcggttg
     3721 gcgatcatcg cgtgcag
//
LOCUS       DAOQNN010000317         3703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16595_length_3703_cov_10.467379, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000317 DAOQNN010000000
VERSION     DAOQNN010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16595_length_3703_cov_10.467379"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="trpD"
                     /locus_tag="PKV97_13685"
     CDS             complement(<1..681)
                     /gene="trpD"
                     /locus_tag="PKV97_13685"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNB06979.1"
                     /translation="MTTITAQQALQRTIEHREIFFDEMLSLMRQIMAGEISPVMTAAI
                     LTGLRVKKETIGEITAAATVMREMSTKVVVEPPHDHFLDVVGTGGDGANTFNISTTTI
                     FVAAAAGARVAKHGGRGVSSKSGAADVIEALGVNLDLTPQQVAECIEATGIGFMYAPA
                     HHSAMKNVAPVRREMGVRTIFNILGPLTNPAGAPNTLMGVFHPDLVGILVRVLQRLGS
                     EHVMVVYGM"
     gene            complement(703..1290)
                     /locus_tag="PKV97_13690"
     CDS             complement(703..1290)
                     /locus_tag="PKV97_13690"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNB06980.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGAKVKVYRNDEITLEQIGRLAP
                     EHLVISPGPCSPAQAGISVAAIKAFAGKLPILGVCLGHQSIGEAFGGRVVHAKRLMHG
                     KTSPVHHIDQGVFRGLPNPLTCTRYHSLAIERASLPDCLEVTAWTEDGEIMGVRHRTL
                     DVEGVQFHPESILTECGHALLKNFLDRGASQPAAA"
     gene            complement(1376..2848)
                     /gene="trpE"
                     /locus_tag="PKV97_13695"
     CDS             complement(1376..2848)
                     /gene="trpE"
                     /locus_tag="PKV97_13695"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNB06981.1"
                     /translation="MLEHEFNALAAEGYNRIPLTLETFADLDTPLSIYLKLANEPYTY
                     LLESVQGGERFGRYSFIGLSSPTRIEVYGRSALLLTGNRLVERRDYGDPLNFVAEFMN
                     RIKVPPRQHLPRFAGGLVGCFGYDTVRYIEPRLAKTEKADTVGTPDILLLLSEEVAIV
                     DNLSGKLTLVVYAEPEVPGAYKRAQRRLRELLARLREAVEIPEEFRGESAEPVSSFGE
                     EAFKDAVRRAKQYIVDGDLMQVVLSQRMSKPYAASPMALYRAIRSLNPSPYLFYFNLE
                     DFHVVGASPEILTRLEDDVVTVRPIAGTRKRGATPAEDLALEQELLADQKEIAEHVQL
                     LDLGRNDAGRVSEVGTVRVTERFTVERYSHVMHIVSNVEGKLREGLDALAVLRATFPA
                     GTVSGAPKVRAMEIIDELEPVKRGIYAGAVGYLGFHGDMDLAIAIRTAVVKDGQIHVQ
                     AGAGIVADSDPDAEWNETQSKARAMLRAAEMAEGGLDTRF"
     gene            complement(3162..>3703)
                     /locus_tag="PKV97_13700"
     CDS             complement(3162..>3703)
                     /locus_tag="PKV97_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNB06982.1"
                     /translation="IHSFVGKGIPNLVRRCLTEGTRASEDEIDAAVGVFRRHYAVVNG
                     ARTRIYPGVAELLDHLRGRGLPLAVVTNKAAAFTLPLLERMAIAHYFDAVVSGDTLPV
                     KKPDPAMLHLACARLGVAARQALMVGDSANDAFAARDAGMPVLLVRYGYSEGMPVDSI
                     ECDGLLSNALEVLDHIGPA"
BASE COUNT          551 a         1153 c         1400 g          599 t
ORIGIN      
        1 catgccgtag accaccatca cgtgctcgct gcccaggcgc tgcagcacgc gcaccaggat
       61 gcccacgagg tcggggtgga acacgcccat cagcgtgttg ggcgcgccgg ccgggttggt
      121 gagcgggccg aggatgttga agatggtgcg cacacccatc tcgcgccgca ccggggcgac
      181 gttcttcatc gcgctgtggt gggcgggcgc gtacatgaag ccgatgccgg tggcctcgat
      241 gcactcggcg acctgctgcg gggtgagatc caggttgacg ccgagcgcct cgatcacgtc
      301 ggccgcgccc gacttggacg acaccccgcg tccgccatgc ttggcgacgc gcgcgccggc
      361 ggccgcggcg acgaagatcg tggtggtgga gatgttgaag gtgttggcgc cgtcgccacc
      421 ggtgccgacg acgtcgagga agtggtcgtg cggcggctcg accacgacct tggtggacat
      481 ctctcgcatc accgtggcgg cggcggtgat ctcgccgatg gtctccttct tgacgcgcaa
      541 gccggtgagg atggcggcgg tcatcaccgg ggagatctcg ccggccatga tctggcgcat
      601 cagcgagagc atctcgtcga agaagatctc gcggtgttcg atcgtgcgtt gcagggcttg
      661 ctgggcggta atcgtggtca tggggctctc gcagcgttcg tttcaggcgg ccgccggctg
      721 gctggcgccg cggtcgagga agttcttcag cagcgcgtgg ccgcactcgg tgaggatgga
      781 ctcggggtgg aactgcacgc cctcgacgtc gagcgtcctg tggcgcacgc ccatgatctc
      841 gccgtcctcg gtccacgccg tcacttccag acaatcgggc aggctggcgc gttcgatcgc
      901 cagcgaatgg tagcgcgtgc aggtgagcgg gttgggcagg ccgcggaaga cgccctggtc
      961 gatgtgatgc accggcgagg tcttgccgtg catgaggcgc ttcgcgtgca cgacgcgccc
     1021 gccgaaggcc tcgccgatgc tctggtggcc gaggcagacg cccaggatgg ggagcttgcc
     1081 ggcgaaggcc ttgatcgccg ccaccgagat gccggcctgg gccggggagc acggcccggg
     1141 cgagatgacg agatgttcgg gcgcgaggcg gccgatctgc tcgagggtga tctcgtcgtt
     1201 gcgatacacc ttgaccttgg cgccgagttc gccgaagtac tgcacgaggt tgtaggtgaa
     1261 gctgtcgtag ttgtcgatca tcagcagcat ggtctttccc ttcgcgaagc cgcgcgccgc
     1321 gcgggcatcg ccagtatcga ggcgtggtgg acggagggcg gcgcaggcgc cgcgctcaga
     1381 agcgggtgtc gagtccgcct tcggccatct cggcggcgcg cagcatcgcg cgcgccttgc
     1441 tctgggtctc gttccactcg gcgtcggggt cggagtcggc gacgatgccg gcgccggcct
     1501 ggacgtggat ctggccgtcc ttcaccaccg cggtacggat cgcgatcgcc aggtccatgt
     1561 cgccatggaa gccgaggtag cccaccgcgc cggcgtagat gccacgcttg accggctcga
     1621 gctcgtcgat gatctccatc gcgcgcacct tgggcgcgcc cgacacggtg cccgcgggga
     1681 aggtggcgcg cagcacggcg agcgcgtcga ggccctcgcg cagcttgccc tcgacgttgg
     1741 agacgatgtg catcacgtgc gagtagcgct cgacggtgaa gcgctcggtg accctgaccg
     1801 tgccgacctc ggagacgcgg ccggcgtcgt tgcggccgag gtcgagcagt tgcacgtgct
     1861 cggcgatctc cttctgatcg gcgagcagtt cctgttcgag cgcaaggtcc tcggccgggg
     1921 tggcgccgcg cttgcgggtg ccggcgatcg gacgcacggt gacgacgtcg tcctcgagcc
     1981 gggtgaggat ctcgggcgag gcgccgacga cgtggaagtc ctcgaggttg aagtagaaca
     2041 ggtagggcga ggggttgagc gagcggatcg cgcggtacag cgccatcggg ctggcggcgt
     2101 agggcttgct catgcgctgc gacagcacga cctgcatgag gtcgccgtcg acgatgtact
     2161 gcttggcgcg gcgcaccgcg tccttgaagg cttcctcgcc gaagctcgac accggctcgg
     2221 cggactcgcc gcggaactcc tcggggatct ccaccgcctc gcgcaggcgg gcgaggagct
     2281 cgcgcaggcg cctctgcgcg cgcttgtacg cgcccggcac ctcgggctcg gcatagacga
     2341 cgagggtgag cttgccactg aggttgtcga cgatcgcgac ttcttccgac agcagcagca
     2401 ggatgtcggg cgtgccgacg gtgtcggctt tctcggtctt ggccaggcgc ggctcgatgt
     2461 agcgcacggt gtcgtagccg aaacagccca ccagaccgcc ggcgaagcgc ggcaggtgct
     2521 ggcgcggcgg caccttgatg cggttcatga actcggcgac gaagttgagc gggtcgccgt
     2581 agtcgcgacg ctcgaccagg cggttgccgg tgagcagcag ggcggagcgg ccatagacct
     2641 cgatgcgggt gggcgaggac aggccgatga aggaatagcg cccgaagcgc tcgccgccct
     2701 gcaccgattc gagcaggtag gtgtagggct cgttggcgag cttcaggtag atggagagcg
     2761 gggtgtcgag gtcggcgaag gtctcgaggg tgagcgggat gcggttgtag ccctcggcgg
     2821 cgagggcgtt gaattcgtgt tcgagcatgg gggctccacg tgggacggat cgatcggaag
     2881 ggcttgcagc gggcggggaa ggcgtcgcgc agcgtgcgcg gcgggccccg cagggggagg
     2941 tcgcgttcag tggcccaaag gccaacgcca gccgccgcgc cagtcgggct gggcggagga
     3001 cgaggcggag aacaggcgat cccggggtgc gagcatcacg atgaagtcgt tggtggagag
     3061 acggacgagg gcggatgaaa gggcgccggc gcagccaccg gaccttgcga gcccgcagcg
     3121 ggattgcgag ggattgcgcc gcccctgccg ccggtttgcg cctatgcagg accgatatgg
     3181 tccagcactt cgagtgcgtt cgatagtagc ccatcgcatt cgatgctgtc caccggcatg
     3241 ccttcgctgt agccgtaacg caccagcagc accggcatgc ccgcgtcgcg cgcggcgaag
     3301 gcgtcgttgg ccgagtcgcc gaccatcagc gcctgccggg cggccacgcc gaggcgcgcg
     3361 caggccaggt gcagcatcgc cgggtcgggt ttcttgaccg gcaaggtgtc gccgctgacc
     3421 acggcgtcga aatagtgcgc gatcgccatg cgctcgagca gcggcagcgt gaatgccgcg
     3481 gccttgttgg tgaccaccgc cagcggcaga ccgcgtccgc gcaggtgatc gagcagctcc
     3541 gcgaccccgg ggtagatgcg cgtgcgcgcg ccgttgacca cggcgtaatg gcggcggaat
     3601 acccccacgg cggcgtcgat ctcgtcctcg ctggcgcgcg tgccttccgt gaggcagcgc
     3661 cgcaccaggt tgggaatgcc cttgccgacg aagctgtgga tct
//
LOCUS       DAOQNN010000318         3702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16604_length_3702_cov_8.998355, whole genome shotgun sequence.
ACCESSION   DAOQNN010000318 DAOQNN010000000
VERSION     DAOQNN010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16604_length_3702_cov_8.998355"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1858
                     /locus_tag="PKV97_13705"
     CDS             167..1858
                     /locus_tag="PKV97_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNB06983.1"
                     /translation="MKLEFRPKLFDTLPGYSRATFVGDLSSGITVGVLALPLAMAFAI
                     ASGMSPTAGIWTAIVAGFLIAFLGGSKVQIGGPTGAFIPIIYAIVADLGVQNLLIATM
                     MSGVLLFAMGALRLGNMIRFIPLSVVIGFTNGIAVVIFISQIKDFLGLQIADLPGEFF
                     AKMGALAAALPTLHLPTVAVALVSLAILVAWNRQAVKTAWMRRLPGPLAVLIILTAIN
                     ALIALPVDTIGSRFGGIPQEIPPFGLPELSLSTLGKLIAPAITIALLGAIESLLSARV
                     ADSQIDDRHDPNQELMAQGIANVAAPLFGGFAATGAIARTATNIRTGGRTPIAGMIHA
                     GVLLAIVLALAPLASHIPLATLSAIVVIVSINMGEWHAFAPKELARYSLNYRTILLGT
                     FFITVVFDLTLAVEIGMALASLFFIYRMSDLTRIDRVPLEDFYGVEALSHPDGTPRIV
                     AYKLKGSLFFGAANKLENLLLGLPGHPEVVVLDLDKVINIDTTGLDILQTLHRNLQKR
                     GAHLVLCGLNSQPGSIVFRSGFSDKLGDDNIAANLTEALIRAQRLSGREPEIAYA"
     gene            1893..2219
                     /locus_tag="PKV97_13710"
     CDS             1893..2219
                     /locus_tag="PKV97_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNB06984.1"
                     /translation="MPDGKSPLPAASAPAADAIARRLRIHGRVQGVWYRGSATAEAAR
                     LGLSGWVRNRSDGTVEALAIGPLVKVETFVAWAHRGPENARVSRVEVFEAELERLTGF
                     EQRPTV"
     gene            complement(2439..>3702)
                     /gene="dnaE"
                     /locus_tag="PKV97_13715"
     CDS             complement(2439..>3702)
                     /gene="dnaE"
                     /locus_tag="PKV97_13715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585092.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time; main
                     replicative polymerase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNB06985.1"
                     /translation="EQLFDLMTKFAEYGFNKSHTAAYAVVTYHTAWLKAHHCAAFMAA
                     TMSSDLDNTDTVKIFYEDTLANGIKVLPPDVNASDYRFVPVDRKIIRYGLGAVKGVGE
                     PAVRAILAAHEKGGDFRDLFDFCERVDRRMVNRRVIEALIRAGAMDTLPGHKGLDRAQ
                     LMATVALAMEAAEQAAANAMQGGLFDLMPEAAGAAPEFAKVRAWTERERLKEEKLAIG
                     FFLSGHPFNAFKPEVRRFVRRTLAQLEPSRDITMLAGVVMEQRTKVGNRGKMAFVLLD
                     DGTEPREVTVYSEVLEASRGKIVTDEVLVVEAKVSNDDFSGGLRINADRLLTLGEARS
                     RFARALSLRIDGELAASGGAAGAAGKLQTLLEPFREGGCPIRVRYRNAAAEAELPLGD
                     GWRVRLDDALLESLREWLPGEAVEVVYP"
BASE COUNT          549 a         1388 c         1180 g          585 t
ORIGIN      
        1 ccgagtcgcc gtcatgccgc tcacaccctc gaaacgctgg atcgtcctgc atcacgccgg
       61 ccgagggtga ccgtctccgc gctccgcgcc gcctgccagc cctcgacgcc ggcggcgccg
      121 cccgggcctc gcgccccgtc ctgaaccagt acgctgcgga gacaacatga aactcgaatt
      181 ccgtcccaag ctcttcgaca ccctgcccgg ctacagccgt gcgaccttcg tcggcgacct
      241 ctcctcgggc atcaccgtgg gcgtgctcgc gctgccgctc gcgatggcgt tcgcgatcgc
      301 cagtggcatg tcgcctaccg cgggcatctg gaccgcgatc gtcgccggct tcctgatcgc
      361 cttccttggt ggctccaagg tgcagatcgg cgggcccacc ggcgccttca tcccgatcat
      421 ctacgccatc gtcgccgacc tcggcgtgca gaacctgctc atcgccacca tgatgtcggg
      481 cgtgctgctg ttcgccatgg gcgcgctgcg cctgggcaac atgatccgct tcatcccgct
      541 gtcggtcgtg atcggcttca ccaacggcat cgcggtggtg atcttcatct cgcagatcaa
      601 ggacttcctc ggcctgcaga tcgccgacct gccgggcgag ttcttcgcca agatgggggc
      661 gctcgccgcg gcgctgccca cgctgcacct gcccacggtg gcggtggcgc tcgtgtcgct
      721 cgcaatcctg gtggcgtgga accgccaggc cgtgaagacc gcgtggatgc gccgcctgcc
      781 cggcccgctc gcggtactga tcatcctgac cgcgatcaac gccctgatcg cgctcccggt
      841 cgacaccatc ggcagccgct tcggcggcat cccgcaggaa attccgccct tcggcctgcc
      901 ggaactgagc ctgtccacgc tcggcaagct gatcgcgccg gcgatcacga tcgcgctgct
      961 cggcgcgatc gagtcgctcc tctcggcgcg cgtcgccgac agccagatcg acgaccgcca
     1021 cgaccccaac caggagctga tggcgcaggg catcgccaac gtcgccgcgc cgctcttcgg
     1081 cggcttcgcc gccaccggcg cgatcgcgcg caccgcgacc aacatccgca ccggcggacg
     1141 caccccgatc gccggcatga tccacgccgg cgtgctgctc gcgatcgtgc tcgcgctcgc
     1201 cccgctcgcc agccacatcc cgctcgccac gctgtcggcg atcgtggtca tcgtgtcgat
     1261 caacatggga gagtggcacg ccttcgcgcc gaaggagctc gcgcgctact cgctcaacta
     1321 ccgcaccatc ctgctcggca ccttcttcat caccgtggtg ttcgacctca ccctggcggt
     1381 cgagatcggg atggcgctcg cgagcctatt cttcatctac cgcatgtccg acctcacccg
     1441 catcgaccgc gtgccgctgg aagacttcta tggcgtcgaa gccttgtccc accccgacgg
     1501 cacgccgcgc atcgtcgcct acaagctcaa gggcagcctc ttcttcggcg ccgccaacaa
     1561 gctcgagaac ctgctgctcg ggctgcccgg ccaccccgag gtggtcgtgc tggacctcga
     1621 caaggtgatc aacatcgaca ccaccggcct ggacatcctg cagaccctgc accgcaacct
     1681 gcagaagcgc ggcgcccacc tcgtgctgtg cggactcaac tcgcaacccg gctcgatcgt
     1741 gttccgctcg ggcttcagcg acaagctcgg cgacgacaac atcgccgcca acctcaccga
     1801 ggcgctgatc cgcgcccagc gcctgagcgg gcgcgagccg gagatcgcct atgcctgagc
     1861 ggaaatcgcc cacgcctgaa cagcactcga gcatgcccga cgggaaatca ccgctgcccg
     1921 ctgcaagcgc gccggcggcg gacgccatcg cccgccgcct gcgcatccac ggcagggtgc
     1981 agggcgtctg gtatcgcggc agcgcgaccg ccgaggcggc gcgcctcggc ctctcgggct
     2041 gggtgcgcaa ccgcagcgac ggaacggtcg aggcgctcgc gatcggcccg ctcgtcaagg
     2101 tcgaaacctt cgtcgcctgg gcccaccggg gcccggagaa tgcgcgcgtg tcgcgggtgg
     2161 aggtgttcga ggccgaactc gagcgcctca ccggcttcga gcagcgtccg acggtttgag
     2221 cacagcccga cccgaacgaa gcgccctcga cttccgcgac ggcgacgcgg cgcgcccgtt
     2281 gcgcgccgtg ggagttcatg cgctgtgatc gcgacttgcg tcgctcctac gaaaacccgc
     2341 agacccccag cgcatgttcg attcccgtgg gagcgacgca agtcgcgatt gcagcgcctc
     2401 cattccacgc cgcagtcgtc accgcaccgg gcggcgcctc aggggtacac cacctcgacc
     2461 gcctcgcccg gcagccattc gcgcaggctc tccagcagcg cgtcgtccag gcgcacgcgc
     2521 cagccgtcgc cgagcgggag ctcggcttcg gcggcggcgt tgcgatagcg gacgcggatc
     2581 gggcagccgc cctcgcggaa gggctcgagc agggtctgca gcttgccggc cgcgcccgcc
     2641 gcaccgccgc tggcggcaag ctcgccgtcg atgcgcagcg acagcgcgcg cgcgaagcgg
     2701 ctgcgcgcct ccccgagcgt gagcaggcgg tcggcgttga tgcgcaggcc gccggaaaag
     2761 tcatcgttgc tcaccttggc ctccaccacc agcacctcgt cggtgacgat cttgccgcgg
     2821 ctcgcctcca gcacctcgga atacaccgtg acctcgcgcg gctcggtacc gtcgtcgagc
     2881 agcacgaagg ccatcttgcc gcggttgccg accttggtgc gctgctccat caccacgccg
     2941 gccagcatgg tgatgtcgcg cgagggctcg agctgcgcca gcgtgcggcg cacgaagcgg
     3001 cgcacctcgg gcttgaaggc gttgaaggga tggccggaga ggaagaagcc gatcgcgagc
     3061 ttctcttcct tgaggcgctc gcgctcggtc cacgcacgca ccttggcgaa ctcgggcgcc
     3121 gcgcccgcgg cctccggcat caggtcgaag agcccaccct gcatcgcgtt ggccgccgcc
     3181 tgttcggccg cctccatcgc cagcgcgacg gtcgccatca gctgcgcgcg gtccaggccc
     3241 ttgtggcccg gcagggtgtc catcgcgccg gcgcggatca gcgcctcgat gacgcggcgg
     3301 tttaccatgc ggcgatcgac gcgctcgcag aaatcgaaca ggtcgcggaa gtctccgccc
     3361 ttctcgtgcg cggcgaggat cgcgcgcacc gcaggctcgc ccacgccctt caccgcgccc
     3421 aggccgtagc ggatgatctt gcgatccacc ggcacgaagc ggtagtcgga ggcgttgaca
     3481 tccggcggca gcaccttgat gccgttggcg agcgtgtctt cgtagaagat cttgacggta
     3541 tcggtgttgt ccaggtcgga cgacatcgtc gccgccatga aggccgcgca gtggtgcgcc
     3601 ttgagccagg cggtgtggta ggtgaccacc gcgtaggccg cggtgtgcga cttgttgaag
     3661 ccgtactccg cgaacttggt catcaggtcg aagagctgct cg
//
LOCUS       DAOQNN010000319         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16611_length_3701_cov_11.693911, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000319 DAOQNN010000000
VERSION     DAOQNN010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16611_length_3701_cov_11.693911"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PKV97_13720"
     CDS             <1..912
                     /locus_tag="PKV97_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06986.1"
                     /translation="GGWERVQRLLAASVEARALRAAEREVAEKQRQEARRLRRRSRAL
                     AVGLSVLAFTSALGGLAWQQSRIAEAARRFAEGERELALTARDEVVRQLEALVEAREA
                     EAAARAAAEAVAQEARQSATALASVVDELERATTAANAPASGDGQAVQRSLTEAKSEL
                     RAQVSNLSNLSAQQAAPPVSAAPAPASGGVAPRLWIYIAEEGQRPTAEALEARLRSVR
                     IGDAALELPGIVLVKAAPARSMLRCFRTEECRQDGEALARALAALLQSPTPSLEDFSA
                     LYGSSGSVRARHYELWFAPGAIVLSAR"
     gene            complement(941..1510)
                     /locus_tag="PKV97_13725"
     CDS             complement(941..1510)
                     /locus_tag="PKV97_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06987.1"
                     /translation="MMSSALAGLYERSGRSPPAALADWEARVDGWCDAYLRVFPDAEL
                     SEINLDLAVFQFDHVSERVTLAYALSVEPLMRRDSGRMRGFPDVNASVRRVLGDRAFV
                     ADKGHFLGHASGGILDINLFPQRRELNRGWSEEGKRFRSMERYVAEHPGTFFYHRPSY
                     RDQTWIPATLEYGVLVDGERWWVDRFRNV"
     gene            complement(1534..2670)
                     /locus_tag="PKV97_13730"
     CDS             complement(1534..2670)
                     /locus_tag="PKV97_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB06988.1"
                     /translation="MADFRKLRVMISSRCASTVRRGDSRVSMTVVRTRLKRELEAESL
                     CGERLFEVWISDNAPDQSQGDLETAWEKSLEEVRKADIVLVLYTGEAGWAPARGLGVC
                     HAEFQQAWNDGPARLKVVRIGDVGTAPKDAAELKRDQAFQAYFDDLNPTSPAASDVDA
                     IVARSREALREAVAGLVKLGGREARKGRFAYGAPLDWSRLDFGHRKKAMEDALGGGLA
                     EFGAVESSGGWLWPLGETSLLTICHGLPGGFGVAAAREMVGQPFLHDHLFLARALGER
                     EAPAGPLHLVACLKGITESQAMRQLGFPDATIVAAPFGVYVSDPVQKIQMIFLANCRD
                     LTTTRNALCRLAEWLNATGEAANLARRALGRRRVVQAILDVQGD"
     gene            complement(2839..>3701)
                     /locus_tag="PKV97_13735"
     CDS             complement(2839..>3701)
                     /locus_tag="PKV97_13735"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNB06989.1"
                     /translation="MCQVKDAPIRDWVKLAVTRARNSGMPAIFWLDPYRPHENELIKK
                     VKTYLKDHDTSGLDIQIMSQVRAMRVTLERVARGLDTISVTGNILRDYLTDLFPIMEL
                     GTSAKMLSIVPLMNGGGMYETGAGGSAPKHVQQLVQENHLRWDSLGEFLALAVSLEDL
                     GIKTGNARAKLLAKTLDEATGRLLDENKSPSPKTGQLDNRGSQYYLARFWAERLAGQA
                     EDAELAGKFAPLAKALAENEQKIVAELAEVQGKAVDIGGYYKADAEKCKAVMRPSATL
                     NAILKGARAA"
BASE COUNT          562 a         1299 c         1276 g          564 t
ORIGIN      
        1 ggcggctggg agcgcgtgca gcgcctgctc gccgccagcg tcgaggcgcg cgccctgcgc
       61 gcggccgagc gcgaggtcgc cgaaaagcag cgccaggagg cacgccgcct gcgccggcgc
      121 agccgcgcgc tcgccgtggg cctcagcgtg ctcgccttca ccagcgcgct cggcgggctc
      181 gcctggcagc agtcgcggat cgccgaggcg gcgcgccggt tcgccgaagg cgagcgcgag
      241 ctcgccctca ccgcacgcga cgaggtggtg cgccagctcg aggccctggt cgaggcgcgc
      301 gaggccgagg ccgccgcacg cgctgcggcc gaggcggtgg cgcaggaggc gcggcaatcc
      361 gccaccgcgc tcgccagcgt ggtcgacgaa ctcgaacgcg ccaccaccgc cgcgaacgcc
      421 cctgcgagcg gcgacggaca ggcggtgcag cgcagcctca ccgaggccaa gtcggagctg
      481 cgcgcccagg tgagcaatct ctccaatctc tcggcgcagc aggccgcacc gccggtctcc
      541 gcagcgcccg ctccggcgtc cggcggcgtg gcaccgcggc tgtggatcta catcgccgag
      601 gagggccagc gcccgaccgc cgaggcgctg gaggcgcgcc tgcgcagtgt gcgcatcggc
      661 gacgccgcgc tcgagctccc cggcatcgtg ctggtcaagg ccgcccccgc gcgcagcatg
      721 ctgcgctgct tccgtaccga ggaatgccgc caggacggcg aggccctcgc gcgtgcgctc
      781 gccgcgctgc tgcagtcgcc cacgccgagc ctggaggatt tcagcgcgct ctacggcagc
      841 agcggttcgg tccgcgcacg ccattacgag ctctggttcg cccccggggc gatcgtgctg
      901 tcggcacgct agcgcggagc ctgcatgcca ggcgcagcgc tcagacgttc ctgaaccgat
      961 ccacccacca acgctcccca tcgaccagga cgccgtattc cagcgtggcg ggtatccagg
     1021 tctgatcgcg atacgaaggg cgatggtaaa agaaggtccc ggggtgctcg gcgacgtacc
     1081 gctccatgct gcggaagcgc ttgccttcct cggaccagcc gcggttcagc tcgcgccgct
     1141 gcgggaacag gttgatgtcc aggatgcctc cgctggcgtg accgaggaaa tgccccttgt
     1201 ccgcgacgaa ggctcgatcc ccgaggacgc ggcgcaccga ggcattgacg tcgggaaagc
     1261 cgcgcatgcg cccgctgtcg cgacgcatga gcggctcgac cgacagcgca taggcgagcg
     1321 tgacccgttc gctcacatga tcgaactgga acacggcgag atcgaggttg atctccgaca
     1381 gctcggcatc ggggaagacc cggagatagg catcgcacca gccatcgacg cgggcctccc
     1441 agtccgccag cgcggccggc gggctgcgac cgctgcgttc atacaggccg gcaagcgcgg
     1501 acgacatcat gctttcctat cgttcgggcg atctcagtcg ccctgcacat cgaggatggc
     1561 ctgcacgacc ctgcgccgac caagcgcacg gcgcgccagg ttcgccgcct cgccggtggc
     1621 gttcagccac tcggccaggc gacagagggc attgcgggtc gtggtcagat cgcggcagtt
     1681 ggcgaggaag atcatctgga tcttctgcac tgggtcggac acatagacac cgaagggtgc
     1741 cgcgacgatg gtcgcatccg ggaagcccag ctggcgcatc gcctggctct cggtaatgcc
     1801 tttcaggcag gcgaccagat gcagcggacc ggccggtgct tcccgttcgc cgagtgccct
     1861 ggcgaggaac agatggtcgt ggagaaaggg ctggcccacc atttcccggg cggccgccac
     1921 gccgaagccc cctggcagac catgacagat ggtgagcagc gaggtttcgc ccagaggcca
     1981 caaccagccc ccgctggatt cgaccgcgcc gaactcggcg agccccccac cgagcgcgtc
     2041 ttccatcgcc ttcttgcggt ggccgaaatc gagccggctc cagtcgagcg gggcaccgta
     2101 ggcgaaacgc cccttgcgtg cctcgcgtcc acccagcttg accagcccgg ccaccgcctc
     2161 gcgcaaggcc tcgcggctgc gtgcgacgat ggcgtcgacg tcggaagcgg ccggcgaggt
     2221 gggattgagg tcgtcgaaat aggcctggaa ggcctgatcg cgcttcaact cggcggcgtc
     2281 cttgggcgcc gtgcccacgt cgccgatgcg caccaccttg agccgcgccg gcccatcgtt
     2341 ccaagcctgc tgaaactccg catggcacac gccgaggccg cgagcgggcg cccagcccgc
     2401 ctcgccggtg taaagcacca ggacaatgtc ggccttgcgc acctcctcga gcgatttctc
     2461 ccacgcggtc tccagatcgc cctgcgattg atccggcgca ttgtcggaga tccacacctc
     2521 gaaaagccgc tcgccgcaga ggctttccgc ttcgagttcg cgcttcagcc gcgtgcggac
     2581 gacggtcatc gaaacccgtg aatccccacg gcgcaccgtg gacgcgcagc ggctggagat
     2641 catcacgcgg agcttgcgga aatcggccat cggcctgtcc tcgtaaggcg aggctgtcat
     2701 gttaggcacc cggcggcgat cgtgcatacc cttgccttgc gtggggacgc gaaaaagggg
     2761 caggtcgacg cattcgtcga tctgcccctt ttcgggttcg ccggccgccg ggcgctggcc
     2821 ggcggcacat caaccgcctc aggcggcgcg cgcgcccttc aggatggcgt tgagcgtggc
     2881 gctcggacgc atcaccgcct tgcacttctc ggcgtcggcc ttgtagtagc cgccgatgtc
     2941 caccgccttg ccctgcacct cggcgagctc ggcgacgatc ttctgctcgt tctcggccaa
     3001 ggccttggcg agcggggcga acttgcccgc cagctcggcg tcttccgcct gtccggcgag
     3061 gcgctcggcc cagaagcggg cgaggtagta ctggctgccg cggttgtcga gctggccggt
     3121 cttgggcgag ggcgacttgt tctcgtcgag caggcggccg gtggcctcgt ccagggtctt
     3181 ggcgagcagc ttggcgcggg cgttgccggt cttgatgccg aggtcctcca gcgacaccgc
     3241 cagggcgaga aactcgccga gcgaatccca gcgcaggtgg ttctcctgca cgagctgctg
     3301 cacgtgcttg ggcgccgagc cgccggcacc ggtctcgtac atgccgccgc cgttcatcag
     3361 cggcacgatc gacagcatct tggcgctggt gcccagctcc atgatcggga acaggtcggt
     3421 gaggtagtcg cgcaggatgt tgccggtcac cgagatggtg tccaggccgc gcgcgacacg
     3481 ctcgagggtc acgcgcatcg cgcgcacctg cgacatgatc tggatgtcga ggccgctggt
     3541 gtcgtgatcc ttgaggtagg tcttgacctt cttgatcagc tcgttctcgt gcggacgata
     3601 ggggtcgagc cagaagatcg ccggcatgcc ggagttgcgc gcgcgggtga cggcgagctt
     3661 gacccagtcg cggatcggcg cgtccttgac ctggcacatg c
//
LOCUS       DAOQNN010000320         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16613_length_3701_cov_8.129457, whole genome shotgun sequence.
ACCESSION   DAOQNN010000320 DAOQNN010000000
VERSION     DAOQNN010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16613_length_3701_cov_8.129457"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..762)
                     /locus_tag="PKV97_13740"
     CDS             complement(28..762)
                     /locus_tag="PKV97_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNB06990.1"
                     /translation="MNAPPLQYRLLKRLYQGRCSTVSRVLLPGRDAPVIVKQLDRGRC
                     APGAPARLQHEFELLGTLELEGIPQALEQLEFERGPALVFADREAHSLRQAMRPGSRE
                     WHDWLALMIRVCELLGRLHEARVIHKQINPDHILVTPSGAPLLVDFGMATRFLREQAD
                     WNTPQLAGHRLPYLAPEQSGRINREIDYRTDYYSFGATLYELLTGQPPFAGREGLELV
                     HCHIAREPRPPHHINRLLQGNRTFMS"
     gene            complement(782..1510)
                     /gene="torR"
                     /locus_tag="PKV97_13745"
     CDS             complement(782..1510)
                     /gene="torR"
                     /locus_tag="PKV97_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HNB06991.1"
                     /translation="MSKETPAMPHIMVVEDDPVSRALLVGYFEKEGYRVSASDNADDL
                     LQRVEQERIALVLLDIRLPGKDGLTLTRELRAASRVGIILVTARDDEIDRIVGLELGA
                     DDYVTKPFNPRELLVRARNLLWRVAQCAGSETREAEQGCFRFEGWTLNPHKRQLVSPD
                     GRIERLPQGEFKLLMALLSHAGQVLSRDQLMDAIHDREWTPNDRTVDVLVGRVRRKLD
                     DNPADPRLILTAHGAGYLFAGELD"
     gene            1763..3355
                     /gene="peaA"
                     /locus_tag="PKV97_13750"
     CDS             1763..3355
                     /gene="peaA"
                     /locus_tag="PKV97_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009310 - amine catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     alpha"
                     /protein_id="HNB06992.1"
                     /translation="MEKERNITSMIAAGLLALAAVVGAPERALAADGAALLAANCLSC
                     HSAQGGSISRVEGQRKTPEGWQMTITRMQEQHGAKVSAEDKRRLIKYLADTRGLAPAE
                     TAGWRYLLEHDNNRVETIDGRYRDMCARCHSGARFALQRRSEDEWKLLMHTHIGLNPT
                     LEFHSLARDRQWFPLAVNEVAPALARDFALDARAWKAWQAAPRTALDGSWRIAGFLPG
                     KGAFEGRMRASAAGEDRYELAIEGRYADGALLSGRGMATVYTGYEWRGSVDIDGVALR
                     QVMAADARGERMHGRQHFADSRRMGGALSALREGAAPMVMAVMPAQLRRGETAELTVV
                     GSGLAGAVSLGKGVRVLEVVSRSADRVVVRAHADGRDGLNDVAVGSARGKGLLAVYGR
                     VARVEVEPANAIARVGGPGDAQMQKVGVAYRAIGWAAGRDGKAGTADDLRLGYLPARW
                     SIEAADEEAEKAHDADFVGHIGADGVFVPGDAGPNPARPKSLGNTGRVNVVATVEAAG
                     ATVEGRGKLDVAVPDFVQRALD"
     gene            3372..>3701
                     /locus_tag="PKV97_13755"
     CDS             3372..>3701
                     /locus_tag="PKV97_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase maturase"
                     /protein_id="HNB06993.1"
                     /translation="MTTLTLIRPNLHRVDGAGRQLLFHVPSSSLFELDGPARDVLALF
                     DEQATLETATLADRLGGRHDALALRETVDELRQLHVLAEHGEDGPIEPPPDPPTRFPL
                     DTLVLNVN"
BASE COUNT          522 a         1245 c         1401 g          533 t
ORIGIN      
        1 acgaatttcg cgccgagctg cttggggtta tgacatgaag gtgcgattgc cctggagcag
       61 gcggttgatg tggtgcggcg ggcgcggctc gcgggcgatg tggcaatgca ccagttcgag
      121 cccttcgcga cccgcgaagg gcggctggcc ggtgagcagc tcgtacaggg tggcgccgaa
      181 gctgtagtag tcggtgcggt agtcgatctc gcggttgatc cgcccgctct gctcgggggc
      241 gaggtagggc aggcgatgtc ccgccagctg cggcgtgttc cagtccgcct gctcgcgcag
      301 gaagcgcgtc gccatgccga aatcgaccag caggggcgcg ccggacgggg tcaccaggat
      361 gtggtccgga ttgatctgct tgtggatgac gcgggcctcg tgcagcctgc cgagcagctc
      421 gcacacccgg atcatcagcg ccagccagtc gtgccattcg cggctgccgg gccgcatcgc
      481 ctggcgcagc gaatgcgcct cgcggtcggc gaacaccagc gccgggccgc gctcgaactc
      541 gagctgctcg agcgcctgcg gaatgccttc cagctcgagc gtgccgagca gctcgaactc
      601 gtgctgcagg cgtgcgggcg ccccgggcgc gcagcgtccg cgatcgagct gcttgacgat
      661 caccggcgcg tcgcgccccg gcagcagcac gcggctcacc gtgctgcagc gcccctggta
      721 gaggcgcttg agcaggcggt attgcagcgg gggcgcgttc atcgcggcgg ccggcgcggg
      781 ttcagtcgag ctcgccggcg aagaggtagc ccgcgccgtg cgcggtcagg atcaggcgcg
      841 ggtcggcggg gttgtcgtcg agcttgcggc gcacgcgccc gaccagcacg tcgaccgtgc
      901 ggtcgttggg cgtccactcg cggtcgtgga tcgcatccat cagctggtcg cgcgacagca
      961 cctggccggc gtggctcagc aaggccatca gcagcttgaa ctcgccctgg ggcaggcgct
     1021 cgatgcggcc gtccggggag accagctggc gcttgtgcgg gttcagcgtc cagccttcga
     1081 agcggaagca gccttgctcg gcctcgcgcg tctcgctgcc ggcgcattgc gccacccgcc
     1141 acagcaggtt gcgcgcgcgc accagcagct cgcgcgggtt gaacggcttg gtgacgtagt
     1201 cgtcggcgcc gagctcgagc ccgacgatgc ggtcgatctc gtcgtcgcgc gcggtcacca
     1261 ggatgatgcc gacgcgcgag gcggcgcgca gctcgcgggt gagcgtgagc ccgtccttgc
     1321 cgggcaggcg gatgtcgagc agcacgagcg cgatgcgctc ctgctcgacg cgctgcagca
     1381 ggtcgtcggc attgtcgctc gcggagacgc gatagccttc cttctcgaaa tagccgacga
     1441 gcaatgcgcg cgagacgggg tcgtcctcga ccaccatgat gtgcggcatc gcgggtgtct
     1501 ccttcgacat cgatcctcct tcgtttgggg tcgaacgttc gcttgtcgtg cgcggatgat
     1561 cccccgaatc cccgtcaccg gcaatccgtc gcgagagggc gtgcgtggga tgttgcacgg
     1621 cgtttacatt cgcgcacaac tcggcacatc ggccgccaag ttcgtttacc ggacggactt
     1681 atcgtgtccg tcatcggaag tccggcgggc cgccacggca cccgcccggg ccgcatgcgc
     1741 aacggagcga ggagcgaagg ccttggaaaa ggaaaggaac atcacgtcga tgatcgcggc
     1801 ggggctgctc gccctggcgg ccgtggtcgg cgcgccggag cgggcgctgg cggcggacgg
     1861 cgcggcgctg ctggccgcga actgcctgtc ctgccacagc gcgcagggcg gcagcatttc
     1921 ccgcgtcgag ggccagcgca agacccccga aggctggcag atgacgatca cccggatgca
     1981 ggaacagcac ggcgcgaagg tctcggccga ggacaagcgc cgcctgatca agtacctggc
     2041 cgacacgcgc gggctggccc cggccgagac ggcgggctgg cgctatctgc tcgagcacga
     2101 caacaaccgc gtcgagacca tcgacggacg ctaccgcgac atgtgcgcgc gctgccactc
     2161 cggcgcccgc ttcgcgctgc agcggcgcag cgaggacgag tggaagctgc tgatgcacac
     2221 gcacatcggc ctcaacccga cgctcgagtt ccactcgctg gcacgcgacc ggcagtggtt
     2281 cccgctcgcg gtgaacgagg tcgcccccgc gctggcgcgc gacttcgcgc tcgatgcgcg
     2341 cgcatggaag gcgtggcagg ccgcgccgcg cacggcgctg gacggcagct ggcggatcgc
     2401 cggcttcctg cccggcaagg gcgcgttcga gggacggatg cgcgcgagcg cggccggcga
     2461 ggaccgctac gaactggcga tcgagggccg ctacgccgac ggggccctgc tctcggggcg
     2521 tggcatggcc acggtgtaca ccggctacga gtggcggggc agcgtcgaca tcgacggcgt
     2581 cgcgctgcgc caggtgatgg cggccgatgc gcgcggcgag cgcatgcatg ggcgccagca
     2641 ctttgccgat tcgcgccgca tgggcggggc gctgagcgcg ctgcgcgagg gtgccgcgcc
     2701 gatggtgatg gcggtgatgc ccgcgcagct gcgccgcggc gagaccgccg agctgaccgt
     2761 ggtcggcagc gggctggcgg gggcggtgtc gctcggcaag ggcgtgcgcg tgctcgaggt
     2821 ggtctcgcgc agcgccgacc gcgtggtggt gcgtgcgcat gccgacggcc gtgacggact
     2881 gaacgacgtg gcggtcggca gcgcgcgcgg caaggggctg ctcgcagttt atgggcgggt
     2941 ggcgcgcgtc gaggtcgagc cggccaacgc gatcgcgcgc gtgggcgggc cgggcgatgc
     3001 gcagatgcag aaggtgggcg tcgcctaccg cgccatcggc tgggcggcgg ggcgcgacgg
     3061 caaggctggt accgccgacg acctgcgcct cggttacctg cctgcacgct ggtcgatcga
     3121 ggccgccgac gaggaggccg agaaggcgca cgatgcggac ttcgtcggcc atatcggtgc
     3181 cgacggcgtg ttcgtcccgg gcgatgcggg accgaacccg gcgcggccga agtcgctcgg
     3241 caacaccggc cgggtgaatg tggtcgccac ggtcgaggcc gcgggcgcga cggtcgaggg
     3301 gcgcggcaag ctcgacgtcg cggtgcccga cttcgtccag cgtgcgctcg attgagcgcg
     3361 gggcggatcc gatgacgacc ctgaccctga tccggcccaa cctgcaccgc gtcgacggcg
     3421 cgggccggca gctgctgttc catgtgccga gcagcagcct gttcgaactc gacggccctg
     3481 cacgcgacgt gctggcgctg ttcgacgagc aggccacgct cgagaccgcg accctggccg
     3541 atcgcctcgg tggccgccac gatgcgctcg ccctgcgcga gacggtggac gagctgcgcc
     3601 agctccacgt gctcgccgag cacggcgagg acggcccgat cgagccgccg ccggacccgc
     3661 cgacgcgctt tcccctcgac accctggtgc tcaacgtcaa t
//
LOCUS       DAOQNN010000321         3698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16634_length_3698_cov_8.456492, whole genome shotgun sequence.
ACCESSION   DAOQNN010000321 DAOQNN010000000
VERSION     DAOQNN010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16634_length_3698_cov_8.456492"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..935
                     /locus_tag="PKV97_13760"
     CDS             51..935
                     /locus_tag="PKV97_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNB06994.1"
                     /translation="MSTTLKPRIPSATVQRERTVERLVTGHPTSDGAGVKLTRVLTQP
                     LQRRLDPFLMLDAFGSDQADDYIAGFPDHPHRGFETVTYMIAGRMLHRDSAGHEGLLE
                     NGGVQWMTAGRGVIHSEIPQQEEGVMEGFQLWLNLPAQDKMSAPWYRDFAAGELPRFT
                     TSAGVEATVIAGESHGVTGAVTRTVTAPLYLDLHLPAGSSFAQPLAADHNVFVYVYRG
                     RVKLGEVAVPVRNMAILANAADRDGVRIEATEDARVLLIAGRPLGEPIAQYGPFVMNS
                     EQEIYQAVADYRAGRLAA"
     gene            complement(1315..2229)
                     /gene="ybiB"
                     /locus_tag="PKV97_13765"
     CDS             complement(1315..2229)
                     /gene="ybiB"
                     /locus_tag="PKV97_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HNB06995.1"
                     /translation="MKYGPIIKEIGRGAKGARDLDTAAAAALFGDILDGAVPELELGA
                     ILLAFRIKSEALPELLGFKQAMDARTVQVAVPEGPRCVVLPTYNGARRQANLMPLVAL
                     LLAREGVPVLIQGRHDFETRVSPFELLAALDLHPAVDAVEASAQLARRHIACIGVDKL
                     LSGLDPLLALRLRMGVRASGHTMAKLVDPCRGRSVRVVAVTHPEYLERMDAFLRADGG
                     RAMLMRGTEGEIYANPRRCPEMKVYVDGEARVAVPAEEGGAPPLAGLPDTPSVADNAA
                     LIRAMLAGEVPVPAPIRAQVAALASVAA"
     gene            2338..3219
                     /locus_tag="PKV97_13770"
     CDS             2338..3219
                     /locus_tag="PKV97_13770"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB06996.1"
                     /translation="MDVGFIGLGLMGRPMALRLLKAGHRVQVWSRRRESMQPLLEAGA
                     GDCASAAEVARRAAVTISMVPDAPDVEQVTLGADGVADGAAPGHIHIDMSTIAPAVAQ
                     SIAERLGARGVIALDAPVSGGEPGAIAGTLTIMVGGDAAACVHVQPLFEAMGKSITRI
                     GEAGAGQVAKACNQIVTGVGVAAVAEALNFAAKSGADGTKVREALLGGFAYSRILENH
                     GQRMLERNFRPGFKAWMHQKDLRIVMGEAHRLGVALPAAAAAAQLFNGMLGCGMGDED
                     SVAMLKLLERMSGGVGE"
     gene            3323..>3698
                     /locus_tag="PKV97_13775"
     CDS             3323..>3698
                     /locus_tag="PKV97_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNB06997.1"
                     /translation="MRVGFIGLGVMGEPMARHLRAAGHALAVWARRPESAAALVAEGV
                     PLCATPSDLAARSDVVITIVTASADVEALAFGVQGLAEGFAPGAVHVDMSTIAPASAR
                     ALAERYATRGVGWVDAPVSGGGQ"
BASE COUNT          532 a         1282 c         1362 g          522 t
ORIGIN      
        1 gacgcggccg acgccagcat ctacccgacc ccggacagag gagcacgcag atgagcacga
       61 ccctcaagcc ccgcatcccc tccgcgaccg tgcagcgtga acgcacggtg gagcggctcg
      121 tgaccggcca tccgacttcg gacggtgccg gtgtcaagct cacccgcgtg ctcacccagc
      181 cgctgcagcg ccggctcgat cccttcctga tgctcgacgc cttcggcagc gatcaggccg
      241 acgactacat cgccggcttc cccgaccatc cccaccgcgg cttcgagacc gtgacctaca
      301 tgatcgccgg ccgcatgctg catcgcgaca gcgccggcca cgagggcctg ctggagaacg
      361 gcggcgtgca gtggatgacc gccggccgcg gcgtgatcca ctccgagatc ccgcagcagg
      421 aagagggcgt gatggaaggc ttccagctct ggttgaacct gccagcacag gacaagatga
      481 gcgcgccctg gtatcgcgac ttcgccgcgg gggaactgcc gcgcttcacc acctccgccg
      541 gcgtcgaggc caccgtcatc gccggcgaga gccatggcgt gaccggcgcc gtcacccgca
      601 cggtcaccgc gccgctctac ctcgacctgc acctgcccgc cggttcgagc ttcgcgcaac
      661 ccctggcggc cgaccacaac gtcttcgttt acgtgtaccg cgggcgggtg aagcttggtg
      721 aggtggcggt gccggtgcgc aacatggcga tcctcgccaa cgccgcggat cgcgacggcg
      781 tgcgcattga ggcgaccgag gacgcccgcg tgctgctgat cgccggccgc ccgctcggcg
      841 agccgatcgc gcaatacggc ccgttcgtca tgaacagcga gcaggagatc taccaagccg
      901 tagccgacta tcgcgcgggc cgcctggccg cctgaagaga gacctgcggt tcgcccgcgc
      961 tggcggcgat cgcgggacag gcgccgcggt cgccccgaaa accgccgcgc ttactttgcc
     1021 ccgctgcgat cgcgacttgc gtcgctccca cgggaaccgc cgggcttccg aagcccggcc
     1081 gcgatcgcga cttgcgtcgc tcctgcggga accgccgcgc ttccgaagcc cggccgtgat
     1141 cgcgacttgc gtcgctcctg cgggaatcgc cgcgcttccg aagcagtgct gtaggagcga
     1201 cgcaagtcgc gattacggtg cttcaggccg cgccgtcgcg tgctgctccg gctcccggcc
     1261 gcatcgcgac ttgcggagcg gctcgggaac gatgggaagt cgggtcggat gcgctcaggc
     1321 ggccacggat gcgagggcgg cgacctgggc gcggatcggc gccggcacgg gaacctcgcc
     1381 cgcgagcatg gcgcggatca acgccgcgtt gtccgccacc gagggcgtat ccggcagccc
     1441 ggcgagcggg ggggcgccgc cttcctctgc ggggacagcg actctggcct cgccatccac
     1501 gtagaccttc atttccggac aacgtcgcgg gttggcgtag atctcgccct cggtgccgcg
     1561 catcagcatc gcgcggccgc cgtctgcgcg caggaaggcg tccatgcgct cgaggtactc
     1621 gggatgcgtc accgcgacca cgcgtacgct gcgtccgcgg caggggtcga ccagcttggc
     1681 catggtgtgg ccgctcgcac gcacgcccat gcgcaggcgc agcgcgagca gcggatccag
     1741 cccggacagc agcttgtcca caccgatgca ggcgatgtgc cgccgcgcga gttgtgcgga
     1801 agcctcgacg gcgtcgacgg ccggatgcag gtcgagcgcg gcgagcagct cgaaggggct
     1861 cacccgtgtc tcgaagtcgt ggcggccctg gatcagcacc ggcacgccct cgcgcgcgag
     1921 caacagcgcc accagcggca tcaggttggc ctggcggcgc gcgccgttgt aggtcggcag
     1981 caccacgcag cgcggaccct cgggcaccgc cacctgcacg gtgcgcgcgt ccatcgcctg
     2041 cttgaagccg agcagctcgg gcagcgcctc gctcttgatg cggaaggcga gcaggatggc
     2101 gccgagctcg agttcgggca ccgcgccgtc gaggatgtcg ccgaagagcg ccgccgcagc
     2161 ggccgtgtcg aggtcgcggg cgcccttggc gccccgcccg atttccttga tgattggacc
     2221 gtatttcatg gcgtcgatta tggcgagctt cgcgccgcgc gaccagcgtg tcggcgcttc
     2281 gtgcccctgg cctgcccaat ccgtcgcctc gcaggaaatt ccacggagac tcgacgaatg
     2341 gatgtcggat tcatcgggct cggcctcatg ggccggccca tggcgctcag gctgctgaag
     2401 gcgggccacc gcgtgcaggt gtggagccgg cggcgcgaat cgatgcagcc gctgctggag
     2461 gccggcgcgg gcgactgcgc gagtgcggcc gaggtggcgc ggcgcgcggc ggtgacgatc
     2521 agcatggtgc cggacgcgcc cgacgtcgag caggtgacgc tgggtgccga tggcgtggcc
     2581 gacggtgccg cacccggcca catccacatc gacatgagca cgatcgcgcc cgctgtggcg
     2641 cagtccatcg ccgagcgcct gggcgcgcgc ggggtgatcg cgctcgatgc gccggtttcg
     2701 ggcggcgagc cgggtgcgat cgcgggtacg ctcaccatca tggtgggcgg cgacgcggcg
     2761 gcctgcgtgc acgtgcagcc gctcttcgaa gccatgggca agtcgatcac ccgcatcgga
     2821 gaggccggtg ccggccaggt cgccaaggcc tgcaatcaga tcgtcaccgg ggtgggcgtg
     2881 gcggcggtgg ccgaggcgct caatttcgcg gccaagagcg gcgcggacgg cacgaaggtg
     2941 cgcgaggcgc tgctcggcgg cttcgcatac tcgcgcatcc tggagaacca cggccagcgc
     3001 atgctcgagc gcaacttccg gcccggcttc aaggcctgga tgcaccagaa ggacctgcgc
     3061 atcgtcatgg gcgaggcgca ccgcctcggc gtggcgctgc ccgcggctgc ggccgcggcg
     3121 cagctcttca acggcatgct gggttgcggg atgggggatg aagactcggt ggcgatgctg
     3181 aagctgctgg agcgcatgag cggcggggtg ggtgaatgag cgcgcccgca cggccgcccg
     3241 aagggaagcg ctgtcctctc ccggggagga ggtcgcgcag cgacaggagg gtagtccggt
     3301 gagcgcggcg gggggcaaga cgatgagagt cggtttcatc gggctgggcg tgatgggcga
     3361 accgatggcg cgccacctgc gcgctgcggg ccatgcgctc gcggtatggg cgcggcggcc
     3421 ggagtcggcg gcggcgctgg tcgccgaggg cgtgcccttg tgcgcgacgc cttctgatct
     3481 ggccgcgcgc agcgacgtgg tgatcaccat cgtcaccgcg agcgcagacg tcgaggcgct
     3541 cgccttcggt gtgcaagggc tggccgaggg cttcgcgccc ggggccgtcc atgtggacat
     3601 gagcacgatc gccccggcgt cggcgcgcgc gctcgcggag cgctacgcca cgcgcggcgt
     3661 cggctgggtg gacgcgccgg tgtcgggcgg cggacagg
//
LOCUS       DAOQNN010000322         3694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16662_length_3694_cov_7.361363, whole genome shotgun sequence.
ACCESSION   DAOQNN010000322 DAOQNN010000000
VERSION     DAOQNN010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16662_length_3694_cov_7.361363"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PKV97_13780"
     CDS             complement(<1..641)
                     /locus_tag="PKV97_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HNB06998.1"
                     /translation="MSCAPDSAQDGPSAPEPPAAGQCVANNAGNPCGSASGPASQGST
                     TGQDVGAGNPIDVLSGNKYQQEVDLPALPGILGLEIVRHYNSRRAHPGDIGALGSGWR
                     LSYETRLNIRDDRLEILQADGARVIFARRPGEPQRCASRDPSRGEILLRHRPGGGDEY
                     LWRWPDGRSLLFDSAGHLVQILVASGEFVSLRHDAAGRLLQVTDPQGRSLQVH"
     gene            complement(899..1936)
                     /locus_tag="PKV97_13785"
     CDS             complement(899..1936)
                     /locus_tag="PKV97_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNB06999.1"
                     /translation="MMNPAHPLPPARRPLPRLLPRPWRHRQRGAGAAEFVIVAPALLA
                     LTLAMLQTGLAFHARSIVNYASFEAARAGSIANAGLASMRLAFARAMTPYYGGGRNLA
                     ELADAFAAAQLDLAAALRIEIVSPTRESFDDYHSLAAARRLQTTARVIPSAQPGVLGC
                     PADRANCNADPASNRSGQTLADANLLKLRVTWGLPPAKQVPLAGRFFTWALRMLDPDD
                     DDAFRRGLVEAGRIPMVSQVTLRMQSDAIENEAMRPRTAGSATPPAADSTPLPGCTPT
                     APLCTPEPATADTAPEAPDPTGEDGTPAGDGSGSDGEPGPGTDSGDETEDAEDAEDAE
                     DAEDAEEEPEC"
     gene            complement(1933..2259)
                     /locus_tag="PKV97_13790"
     CDS             complement(1933..2259)
                     /locus_tag="PKV97_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNB07000.1"
                     /translation="MRVLVARSFLQRARGLLGRTHLAADEALLLRPCSSVHTFGMCFA
                     IDVVFIDPQGVVVAVREALGPWRFARCRGAAAVLEMAAGAARRAGLVPGTHLRLASTL
                     SVVVRA"
     gene            complement(2262..3167)
                     /locus_tag="PKV97_13795"
     CDS             complement(2262..3167)
                     /locus_tag="PKV97_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNB07001.1"
                     /translation="MSYAHLLVLDERTVIAAVALLAGLGSAGIVAALARSVSAVRPED
                     RTWLDTPPRFLRLLWWPTRWIAHLVAPLLPARLQARLAVRLRLAGLDYTLSPAQLLAH
                     RVAVGLCGLAAGLGCAHAWALPPALPALAGATAGTLIAWSWLDDRIRCRRRLMLKQLP
                     FVLDLITLCVEAGLNLTGALQQAAAKGPAGPLGEELHRVLRDVRAGKSRADALRGFAD
                     RIGEPAIANLVSTVIQAENMGMSLGPMLRAQGEQRRAERFARAEKAAMEAPVKMLLPL
                     IACIFPCTFIVLGFPIAVKFMTMGL"
     gene            complement(3164..>3694)
                     /locus_tag="PKV97_13800"
     CDS             complement(3164..>3694)
                     /locus_tag="PKV97_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNB07002.1"
                     /translation="IEQQLPDALQGIAGGLRAGVSMTVALQQLVREGRPPIAQEFDLT
                     LREHRLGIPLDEALDHLAARVRMPSLTLVIAAMRIANETGGSLAEALERAALTVRSQL
                     AMEGKIGALTAQGKLQAVVVGMLPLALLLVLDRMEPEAMGFLWHSPPGWATLAVIATL
                     EVLGVLLIRRIVAIDV"
BASE COUNT          512 a         1246 c         1420 g          516 t
ORIGIN      
        1 tagtgaactt gcaggctgcg gccctgcggg tcggtgacct gcagcagccg gccggcggcg
       61 tcgtggcgca gtgagacgaa ctcgccgctc gcaacgagga tctgcaccag gtggccggcg
      121 ctgtcgaaca gcaggctgcg gccgtcgggc cagcgccaga gatattcgtc gccgccgccg
      181 gggcggtgcc ggagcaggat ctcgccgcgg ctggggtcgc ggctggcgca gcgctgcggc
      241 tcaccgggcc ggcgcgcgaa gattacccgc gcgccgtcgg cctggaggat ttcgaggcga
      301 tcgtcgcgga tgttcaggcg cgtttcgtag gacagtctcc agccgctgcc gagcgccccg
      361 atgtcgcccg ggtgcgcgcg tcggctgttg tagtggcgga cgatctcgag gccgaggatg
      421 cctggcagcg ccggaagatc gacctcctgt tggtacttgt tgcccgagag gacgtcgatc
      481 gggtttcccg cgccgacgtc ctggccggtg gtggaaccct ggctggccgg gccgctggcg
      541 ctgccgcagg ggttgcccgc gttgttggcg acgcattgcc ccgccgcggg gggctctggc
      601 gccgaggggc cgtcctgcgc agagtccggc gcacagctca tcgtgattcc gcacccgctc
      661 tgctggatgc cgtgatccgc ggcgccggat tgcgccgccg aggtttcggc cgctaccccg
      721 gggagcgccg tgatacagag cgccgccagc agtgcggcgc ccgcccgcat cgcatggtgc
      781 agccgaccgg ctggcggctg caggcatgcg gacgagctgc gcatcggttg ccgccgcgcc
      841 ggagtggtcc ggcggtacaa ggccgcgggg gcatcgcatg aaagcgtggg ggcagcgttc
      901 agcactcggg ttcctcctcc gcgtcctcgg cgtcctccgc gtcctcggcg tcctccgcgt
      961 cctcggtttc gtcgccgctg tcggttcccg gccccggctc accgtcggat ccgctgccgt
     1021 cgccagccgg cgtaccgtct tccccggtcg ggtccggcgc ctcgggtgca gtgtccgcgg
     1081 tcgcaggttc gggcgtgcac agaggggccg tgggggtgca gccgggcagg ggcgtcgaat
     1141 cggcggctgg gggggtggcg cttcccgccg tacggggccg catcgcctcg ttctcgatcg
     1201 cgtcggactg catgcgcagc gtgacctggc tcaccatcgg gatgcggccg gcctccacca
     1261 ggccgcggcg gaaggcgtcg tcgtcgtccg gatcgagcat gcgcagggcc caggtgaaga
     1321 atcgtccggc gaggggaacc tgcttggccg gcggcagccc ccaggtgacg cgcagcttga
     1381 gcaggttggc gtcggcgagt gtctggcccg agcggttgct tgccggatcg gcgttgcagt
     1441 tcgcgcggtc cgccgggcag ccgagcacgc ccggctgcgc ggacggaatc acccgcgccg
     1501 tggtctgcag gcggcgcgcg gccgccaggc tgtggtagtc gtcgaagctc tcgcgggtgg
     1561 gggagacgat ctcgatgcgc agggcggcgg cgagatcgag ctgcgccgcg gcgaaggcgt
     1621 cggcgagctc ggcgaggttg cgaccgccgc cgtaataggg ggtcatcgcg cgcgcgaagg
     1681 ccaggcgcat gctcgccagt ccggcgttgg cgatgcttcc ggcgcgcgcg gcctcgaagc
     1741 tggcgtaatt gacgatgctg cgggcatgga aggcgagccc ggtctgcagc atcgccagtg
     1801 tgagcgcgag cagggccggg gcgacgatga cgaactcggc cgcgccggcg ccccgctggc
     1861 ggtggcgcca aggccgtgga agaagccgtg gaagaggccg ccgcgccggc ggcagcggat
     1921 gggcgggatt catcatgcgc gcactaccac cgacaaggtg gaagccagcc tgagatgcgt
     1981 gccggggacg agtcccgccc gtctcgcggc gccggccgcc atctcgagca cggcggcggc
     2041 gccgcggcaa cgcgcgaaac gccacgggcc gagcgcctcg cgcaccgcga caacgacgcc
     2101 ttgcgggtcg atgaacacca cgtcgatggc gaagcacatg ccgaaggtgt ggaccgacga
     2161 gcatggacga agcagcaagg cctcgtcggc ggcgaggtgc gtgcgtccga gcaggccgcg
     2221 ggcgcgttgc aggaagctgc gggcgacaag gacgcgcatg ctcacagccc catggtcatg
     2281 aacttcaccg cgatcggaaa gccgagcacg atgaaggtgc aggggaagat gcaggcgatc
     2341 agtgggagca gcatcttcac gggcgcttcc atcgccgcct tctcggcgcg ggcgaagcgc
     2401 tccgcgcggc gttgctcgcc ctgcgcgcgc agcatcgggc ccaggctcat gcccatgttc
     2461 tcggcctgaa tgaccgtgga gaccaggttc gcgatcgccg gttcgccgat gcggtcggcg
     2521 aaaccgcgca gcgcgtcggc gcgcgacttg cccgcacgca cgtcgcgcag cacgcggtgc
     2581 agttcttcgc cgagtggtcc ggcagggcct tttgcggccg cctgctggag tgccccggtg
     2641 aggttgaggc cggcctcgac gcacagggtg atgaggtcga gcacgaaggg gagctgcttg
     2701 agcatcagcc ggcgtcggca gcggatgcga tcgtccagcc aggaccacgc gatcagtgtg
     2761 ccggcggtgg cgcccgccag cgcgggaagc gccggaggaa gtgcccatgc gtgggcacac
     2821 ccgaggccgg ctgccagccc gcacagcccg acggcgaccc ggtgtgcgag cagctgcgcc
     2881 ggagagagcg tgtagtcgag tccggcaagg cgcagtcgca cggccagccg tgcctgcagc
     2941 cgcgccggca gcagcggtgc gaccaggtgc gcgatccagc gcgtgggcca ccacagcagg
     3001 cgcaggaagc gcggtggcgt gtcgagccag gtgcggtcct cgggccggac ggcgctgacg
     3061 ctgcgggcca gcgcggcgac gatgccggcg cttccgagac cggcgagcag ggccaccgcc
     3121 gcgatcaccg tgcgttcgtc gagcacaagc aagtgcgcgt agctcatacg tcgatcgcca
     3181 cgatgcgccg gatcagcagc acgccgagca cctcgagcgt ggcgatcacc gccagcgtcg
     3241 cccagccggg cggcgagtgc cacaggaagc ccatcgcctc cggttccatg cggtcgagca
     3301 ccagcagcag cgccagcggc agcatgccga ccaccaccgc ctgaagcttg ccctgtgcgg
     3361 tgagggcgcc gatcttgcct tccatcgcga gctggctgcg tacggtcagc gccgcgcgtt
     3421 cgagcgcttc ggccaggctg ccgccggtct cgttggcgat ccgcatcgcg gcgatcacga
     3481 gggtcagcga gggcatgcgc acgcgtgcgg cgagatggtc gagcgcctcg tcgagcggaa
     3541 tgccgaggcg gtgctcgcgc agggtgaggt cgaactcctg ggcgatcggc gggcgccctt
     3601 cacggacgag ttgctgcagc gccacggtca tcgaaacccc cgcgcgcagg ccgccggcga
     3661 tgccctgcag cgcgtcgggc agctgctgtt cgat
//
LOCUS       DAOQNN010000323         3693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16669_length_3693_cov_10.929632, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000323 DAOQNN010000000
VERSION     DAOQNN010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16669_length_3693_cov_10.929632"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PKV97_13805"
     CDS             <1..1201
                     /locus_tag="PKV97_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB07003.1"
                     /translation="LALPVLARLVLPLLARGRSAIWRLAHARLAAAPGQAVVAGAGVI
                     ASVALAVSMAIMVNSFRVSVDEWLAQVLPADLYVRASAASGSGYLDPPALVAIAATPG
                     VARVETTRLVNLRVSDTEPLFSVLARATQGNRGLPLVSGSMEPVAGDAEGLPPAWVSE
                     AAADRHDLARGSRFELPLGGRMQAFRVAGVWRDYARQHGAALIERRDYVALTGDDRVN
                     DAALLLEPGATPSAVAQALRERFGAEHITIALPGEIRALSLQIFDRTFLITYLMEAVA
                     VVIGLFGIATTFAALAATRQGEFGMLRHLGFTRAEIGRLIATEGALTAGLGVIAGLAG
                     GAAIAWVLIEIINRQSFHWSMELAVPWSGLAIFAVCLVLLAALVARLAGRHAMRRSAV
                     LAVKADW"
     gene            1326..2399
                     /locus_tag="PKV97_13810"
     CDS             1326..2399
                     /locus_tag="PKV97_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HNB07004.1"
                     /translation="MLVCGGAAQAQDSGPPYPPVVPGKALVFPQDFGAHPAYRTEWWY
                     ITGWLRDAAGVERGFQLTFFRVRTRIGEDNPSRFAPRQLVLAHAAVADPATGRLRHAE
                     RSGRAYPGLVEALEGRTAVRVDDWFLEGGESPAAGYRSRIEAEDFGFELEFTPDRAPV
                     PNGRDGYSQKAPDPLNASYYYSRPQLAVSGSLRLDGEPVAVSGHAWLDHEWSSEILPE
                     GARGWDWIGINLGDGGGLMAFRMRDHEGRALWAAATTSTRDGSVRIHPPTEVRFEPLR
                     RWRSPRTGIEYPVEWAVSVGERRYRLRPLMDDQELDSRASTGAIYWEGAVRLHEAGEG
                     AEEGPELGRGYLEMTGYAERLRM"
     gene            complement(2565..3149)
                     /locus_tag="PKV97_13815"
     CDS             complement(2565..3149)
                     /locus_tag="PKV97_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07005.1"
                     /translation="MEINQKKRSNRLRFTFNDDSFNYAYKDRSGSGDIDVQYAGFPTK
                     ASVSIERNEWLRNAGLIWCVIGVLQLGHAMVTGDSLQGRGFWLLIGVICLVWYRWATV
                     KYSVFRSQAGNVLVIQDREHDRIVDEINRRRKDQLLALYGELDMRNTLEDEIAKFSWL
                     HEQDVLSKDELEGRLAQARSAFQAPGPAASSQLH"
     gene            complement(3183..>3693)
                     /locus_tag="PKV97_13820"
     CDS             complement(3183..>3693)
                     /locus_tag="PKV97_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07006.1"
                     /translation="LAFLIVRGAVEHGSLVLRTDPARDAVFVTTALTPPASGFSLSPG
                     YTLEGHILETGERARLAVSRDVYIRAAPGARHTVLRTNDPATPWVLRATFNPDEAVLR
                     VGDGGVAWFALVALLPVALWGVLVFYPWVRARGEGRALMKEQMSRNLLSITKLTLLLG
                     VVALIKRLA"
BASE COUNT          544 a         1231 c         1343 g          575 t
ORIGIN      
        1 cctggcgctg ccggtgctcg cgcgcctcgt gctgcccttg ctcgcgcgcg ggcgcagcgc
       61 gatctggcgg ctggcgcacg cgcgcctggc ggcggcgccg gggcaggcgg tggtggcggg
      121 ggcaggggtg atcgccagcg tcgcgctcgc ggtgtcgatg gcgatcatgg tgaattcctt
      181 ccgcgtctcg gtggatgagt ggctcgccca ggtcttgccg gcagacctgt acgtgcgtgc
      241 ctcggcggcg agtggcagcg gctacctcga tccgcctgcg ctcgtggcga tcgcggccac
      301 gcccggcgtg gcgcgagtag agacgacgcg gctcgtgaac ctgcgcgttt cggacaccga
      361 gccgctgttc agcgtgctgg cgcgcgcgac gcagggcaac cgggggctgc cgctggtgag
      421 cggatcgatg gagccggtgg ccggggacgc ggaaggcctg cctccggcct gggtgtcgga
      481 ggcggctgcc gaccgccacg atctggcgcg cggcagccgc ttcgagctgc cgctgggagg
      541 ccggatgcag gccttccgcg tcgccggggt gtggcgcgac tacgcccgcc agcacggcgc
      601 cgcgctgatc gagcgccgcg actacgtcgc cctcaccggc gacgaccggg taaacgacgc
      661 cgcgctactg ctcgagccgg gtgccacccc gtcagcggtg gcgcaggcgc tgcgcgagcg
      721 cttcggcgcc gagcacatca ccatcgcgct gccgggcgag atccgcgcgc tcagcctgca
      781 gatattcgat cgtaccttcc tgatcaccta cctgatggag gcggtcgcgg tggtgatcgg
      841 cctgttcggc atcgccacca ccttcgccgc gctcgccgcc acccgccagg gcgagttcgg
      901 catgctgcgc catctcggtt tcacgcgcgc cgagatcggt cgcctgatcg ccaccgaggg
      961 cgcgctgacc gcagggctgg gcgtgatcgc cggactcgcc ggcggcgcgg cgatcgcctg
     1021 ggtgctgatc gagatcatca accggcagag cttccactgg agcatggagc tggccgtgcc
     1081 gtggagcggg ctggcgatct tcgccgtctg cctggtgctg ctggcggcac tggtggcccg
     1141 gctcgccgga aggcacgcga tgcggcggtc ggcggtgctg gcggtgaagg cggattggta
     1201 gggggtgagg cgtgaggagt caggagtcag gagcggtggg ggcgggcagg ctcgcggcgg
     1261 gcgttgcgct tgtggcgctg gagaggcggc ttgtccggct gcgcgtgctg ctcctgggag
     1321 cgctgatgct ggtgtgcggt ggggcggcgc aggcgcagga ttccggaccg ccctacccgc
     1381 cggtcgtgcc gggcaaggcg ctggtgtttc cgcaggattt cggtgcacat ccggcctatc
     1441 gcaccgagtg gtggtacatc accggctggt tgcgcgacgc ggcgggggtg gagcgcgggt
     1501 tccagctcac cttcttccgc gtgcgcacgc gcatcggcga ggacaacccg agccgcttcg
     1561 cgccacgcca gttggtgctc gcgcatgccg cggtggccga cccggcgacc ggcaggctgc
     1621 gccacgccga gcgctcggga cgcgcctatc ccggactggt cgaggcgctg gaagggcgta
     1681 ccgcggtacg ggtggatgac tggttcctcg aaggaggaga gagcccggcg gccggctacc
     1741 gcagccgcat cgaggcggag gacttcggtt tcgagctcga attcaccccg gaccgcgcgc
     1801 cagtgccgaa cgggcgcgat ggctacagcc agaaggcgcc cgatccgctc aatgccagct
     1861 actactacag ccgcccgcag ctcgcggtga gcggctcgct gcgcctggat ggcgagccgg
     1921 tggcggtgag cggccatgcc tggctggatc acgagtggtc gagcgagatc ctgcccgagg
     1981 gcgcgcgcgg ctgggactgg atcggcatca acctcggcga cggcggcggc ctgatggcct
     2041 tccgcatgcg cgaccacgag ggccgcgcgc tctgggcggc cgcgaccacg agcacgcgcg
     2101 atggcagcgt ccgcatccac ccgcccacgg aggtgcgctt cgaaccgctg cgccgctggc
     2161 gctcgccgcg caccggaatc gagtacccgg tggagtgggc ggtctcggtg ggcgaacggc
     2221 gctaccggct gcgcccgctg atggacgacc aggagctcga cagccgcgcc tccaccgggg
     2281 cgatctattg ggagggcgcg gtgcgcctgc acgaggcggg ggagggtgcc gaggagggcc
     2341 ccgagctcgg gcgcggctac ctggagatga ccggctacgc cgagcgcttg cgcatgtaga
     2401 aaccatgaag ggccatccgt tccctctcca cctgaccccg ttgcacggga gctcacggca
     2461 gagcgaaatt ccgtctgacg cgtagaacgc caacgcccgc cggaccagcc acgcgagccc
     2521 ggtgccggca acacctcatc gcgcccgttt cgccagcgca gccgtcaatg aagctggctg
     2581 gatgctgccg gccctggcgc ctgaaaggcc gagcgcgcct gtgcgaggcg tccttccagt
     2641 tcgtccttgg acaagacgtc ctgctcatgc agccaagaga actttgcgat ctcgtcctcg
     2701 agcgtgttgc gcatgtccag ttcgccatag agagcaagga gctggtcctt tcgccggcgg
     2761 ttgatttcat cgacgatccg atcatgctcg cggtcctgaa tcacgaggac gttacccgct
     2821 tgcgagcgga aaaccgaata cttgacggtc gcccagcgat accagacaag gcagatcacc
     2881 ccgatgagaa gccagaagcc tcgcccctga agcgagtcgc cggtgaccat cgcgtgcccc
     2941 aattgcagca caccgatgac gcaccagatc agtccggcat tgcgcagcca ttcgttccgc
     3001 tcgatcgaga ccgaggcctt cgtcgggaag ccggcgtact gcacgtcgat gtcgccggag
     3061 ccgctgcggt ccttgtaggc atagttgaag ctgtcgtcgt tgaacgtgaa cctcaatcgg
     3121 ttggaacgct tcttctggtt gatttccacg gagtcctccg gttcatgggt tgtcttgcgc
     3181 cgtcaggcaa gccgcttgat cagcgccacg acgcccagca gcagtgtcag tttggtaatc
     3241 gacagcaggt tcctgctcat ctgctccttc atcagcgctc gaccttcacc ccgggcccgc
     3301 acccacggat agaacacgag cacgccccac agggcgacgg gcagcagcgc gacgagcgcg
     3361 aaccaggcga cgccgccatc tccgacccgt aagacagcct cgtcggggtt gaacgtcgcc
     3421 ctcaacaccc agggcgtggc gggatcgttg gtgcgcagga cggtgtggcg ggcaccgggc
     3481 gccgcacgga tatacacatc ccgcgacacc gcaagacgag cgcgctcgcc cgtctccagg
     3541 atgtggccct cgagcgtgta gccgggtgac aggctgaagc ccgacgcggg cggtgtcagg
     3601 gcggtcgtga cgaagaccgc gtctctggcc ggatcggtgc gcagcacgag gctgccgtgc
     3661 tcgacggcgc ctcgaacgat caggaacgcg agg
//
LOCUS       DAOQNN010000324         3687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16712_length_3687_cov_7.580121, whole genome shotgun sequence.
ACCESSION   DAOQNN010000324 DAOQNN010000000
VERSION     DAOQNN010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16712_length_3687_cov_7.580121"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PKV97_13825"
     CDS             complement(<1..769)
                     /locus_tag="PKV97_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07007.1"
                     /translation="MKTQLARIARSLCLAFALGAGGVATAASGEAGEVNLPAQELTPR
                     TLYHFLLAEIAGARGQIGLSAQLYLDLARSTRDPRIARRATEIAMYSRNLVMARGAAE
                     IWTEVAPDSDEARRVLAGLSGAGRGEDINLEAIQFQLARVLAQSNGRLAQNLLSLGHT
                     LARVPDKQAVRGIVMRLTEPYVEMPEAHIARAQAAQAVEDRMGALAAVDRALELRSGW
                     EPAVLLKVQILQQAGAHTEALRVLEAEVARAPTSRSLR"
     gene            969..1787
                     /gene="mutM"
                     /locus_tag="PKV97_13830"
     CDS             969..1787
                     /gene="mutM"
                     /locus_tag="PKV97_13830"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNB07008.1"
                     /translation="MPELPEVETTCRGIRPHVQGRSLSRLVVRNPRLRVAVPADLPAH
                     IEGARLEAVDRRAKYLLLRFPAGTVIVHLGMSGSLRVVAAAEPAGAHDHVDFVFGECA
                     LRLRDPRRFGMVVWQPGDVAAHPLLAGLGPEPLGDGFDADWLLRITRGLRAPIKHVLM
                     DSRKLVGVGNIYASESLFRARIHPLEPAGRLGPRRCARLVDCVRETLAAAIAAGGSTL
                     RDFVGGDGRPGYFQQQYFVYGRDGESCRVCGTPVRRIVSAQRASFFCPRCQRRS"
     gene            1953..>3687
                     /locus_tag="PKV97_13835"
     CDS             1953..>3687
                     /locus_tag="PKV97_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNB07009.1"
                     /translation="MTDLTEQFSAYSRWRGHASDAVARLRAWLARNDVGNAQADMRLQ
                     YLLDRLRDDKLTVAFVAEFSRGKSELINAIFFADHGDRILPSSAGRTTMCPTELQWQK
                     GAQPELRLLPIRSRARQTPVSELKRYPEEWTVIALDAVDAPGLQAALARVGETERVEV
                     AEAQALGFRVDAAGDDGIRPDAEGRIEIPRWRHAVIQFPHPLLEQGLVVLDTPGLNAI
                     GAEPELTLSMLPNAHAVLFILAADTGVTQSDLAVWRNYVQAGSERQRGRLVVLNKIDG
                     LWDGLRDEARIEAEIGRQVDSVAATLGVEPAAVFPVSAQKGLVARIQGDEALLARSRV
                     PQLERALAEGLLPAKQEIVREDILAESAEVAAATRSLLEARLAGLREQLQELTDLRGK
                     NQSVIEYMMRKIRSEKEEFEQGLQKYYAVRTVFTDLANNLFAHMGLDAVRDETRRTRE
                     AMLESSFTRGLRNAMQGYFRSLRGNLQRSTEEIGEITRMLDAMYKRFSVEHGLKLTAP
                     EGFSTLRYEKELERLETAFNRQINTALTLVTTEKHALTQKFFETVAVQARQTFELANR
                     DVEQWLRAVMSP"
BASE COUNT          529 a         1318 c         1311 g          529 t
ORIGIN      
        1 ggcgcagcga ccggctcgtc ggcgcgcgcg cgacctcggc ctcgagcacg cgcagggcct
       61 ccgtgtgcgc gcctgcctgc tggaggatct gcaccttcag cagcaccgcg ggttcccagc
      121 cgctgcgcag ctcgagggcg cgatccaccg cagcgagcgc gcccatcctg tcctcgaccg
      181 cctgtgccgc ctgcgcgcgg gcgatgtgcg cctcgggcat ctcgacgtag ggttcggtca
      241 ggcgcatcac gatgccgcgc accgcctgtt tgtcgggtac gcgggcgagg gtatggccga
      301 ggctcagcag gttctgcgcg aggcggccgt tcgattgcgc gagcacgcgc gcgagctgga
      361 actggatggc ctcgaggttg atgtcctcgc cgcggccggc gccgctcagc ccggcgagta
      421 cccggcgcgc ctcgtccgag tcgggggcga cttcggtcca gatctccgcg gcgccgcgcg
      481 ccatcacgag gttgcgcgag tacatcgcga tctcggtggc gcggcgggcg atgcgcgggt
      541 cgcgcgtgct gcgcgcgagg tcgagataga gctgtgccga caggccgatc tggccgcgcg
      601 cgccggcgat ctcggcaagc aggaagtggt acagcgtgcg cggcgtgagt tcctgcgcgg
      661 gcaggttgac ctcgccggcc tcgccgctgg ccgccgtcgc gaccccgccg gcgccgagcg
      721 cgaaggccag gcagagggag cgggcgatgc gggcgagctg ggttttcatg ggaggaattc
      781 cgggaccttc gatgggttgc gccagagccg cggcgcaaga aacgcctggc acccgccggg
      841 gggtgccgcg aaagcttcga gtgctcggct acaattcggt tcatgccatg gtaggcagat
      901 cgccgcccct gccgcaaccc gttccccgtg tgcgcccgcg cgcgggcagc ccacggattc
      961 ctttcccgat gcccgaactg cccgaagtcg agaccacctg ccgtggaatc cggccgcacg
     1021 tccagggccg cagcctgagc cggctggtgg tgcgcaatcc gcgcctgcgc gtggcggtgc
     1081 ccgccgacct gcccgcgcac atcgagggcg ccaggctcga ggcggtcgac cggcgtgcca
     1141 agtacctgct gctgcgtttt cccgcgggca cggtgatcgt gcatctcgga atgtcgggca
     1201 gcctgcgcgt ggtcgcggcg gccgagccgg cgggcgcgca cgaccacgtc gattttgtct
     1261 tcggcgagtg cgcgctgcgt ctgcgcgacc cacgccgctt cggcatggtg gtgtggcagc
     1321 ccggcgacgt ggcggcgcat ccgctgctcg ccggcctggg gccggagccg ctcggcgacg
     1381 gcttcgacgc cgactggctg ctgcgcatca cgcgcggtct gcgcgcgccg atcaagcatg
     1441 tgctgatgga cagccgcaag ctcgtcggcg tgggcaacat ctacgcctcc gagagcctct
     1501 tccgcgcccg catccatccg ctcgagcccg ccggccggct cgggccgcga cgctgcgcgc
     1561 gcctggtcga ttgtgtgcgc gagacgctcg ccgccgcgat cgcggccggc ggcagcacgc
     1621 tgcgcgactt tgtcggcggc gacggccggc cgggctattt ccagcagcag tacttcgtct
     1681 atggtcgcga cggcgagtcc tgccgggtgt gcggcacgcc ggtgcggcgc atcgtcagcg
     1741 cccaacgcgc gagcttcttc tgtccgcgct gccagcgccg cagctgaaag gacggcgatg
     1801 ccccggtggg ggggggcatc aggcggcgtg caatttggca cgctcgcgaa ccgcgaagcg
     1861 ggttatggtg aataatgttc tgcgctgcgg gccggcgagg ccttgccctc gcgcgcgccc
     1921 gacgcccccc cgcctgccgg acgagcacgc cgatgaccga tctcaccgaa cagttttccg
     1981 cctacagccg ctggcgcggc cacgccagcg acgcggtggc ccgcctgcgc gcgtggctgg
     2041 cgcgtaacga cgtgggcaac gcccaggcgg acatgcgcct gcaatacctg ctcgaccgcc
     2101 tgcgcgacga caagctcacg gtggccttcg tcgccgagtt ctcgcgcggc aagtccgagc
     2161 tcatcaacgc gatcttcttc gccgaccacg gcgaccgcat cctgccctcc agcgcgggcc
     2221 gcaccacgat gtgcccgacc gagctgcagt ggcagaaggg ggcgcagccc gagctgcgtc
     2281 tgctgccgat ccgcagccgc gcgcgccaga cgccggtgag cgagctcaag cgctatcccg
     2341 aagaatggac cgtgatcgcg ctcgacgcgg tcgacgcccc cggcctgcag gccgcgctcg
     2401 cgcgcgtggg cgagaccgag cgggtcgagg tcgccgaagc gcaggcgctc ggcttccgcg
     2461 tcgatgccgc aggggacgac ggcatccgtc ccgatgccga gggtcgcatc gagatcccgc
     2521 gctggcgcca tgccgtgatc cagttcccgc atcccctgct cgagcagggc ctggtggtgc
     2581 tcgacacgcc gggcctcaat gcgatcggcg ccgagcccga gctcaccctg tcgatgctgc
     2641 cgaatgcgca cgcggtgctg ttcatcctcg ccgccgacac cggcgtcacc cagagcgatc
     2701 tcgcggtgtg gcgcaactac gtgcaggctg gcagcgagcg ccagcgcggg cgcctggtcg
     2761 tgctcaacaa gatcgacggg ctgtgggacg ggctgcgcga cgaagcgcgc atcgaagccg
     2821 agatcggccg ccaggtcgac tcggtcgccg cgaccctcgg cgtcgagccc gccgcagtgt
     2881 tcccggtgtc ggcgcagaag ggcctggtgg cgcgcatcca gggcgacgag gccctgctcg
     2941 cgcgcagccg tgtgccacag ctcgagcgtg cgctcgccga gggcctgctg cccgccaagc
     3001 aggagatcgt gcgcgaggac atcctcgccg agtccgccga ggtcgccgcc gccacgcgca
     3061 gcctgctcga ggcgcgcctg gccggcttgc gcgaacagct ccaggaactt accgacctgc
     3121 gcggcaagaa ccagagcgtc atcgaataca tgatgcgcaa gatccgcagc gagaaggagg
     3181 agttcgagca ggggctgcag aagtactacg ccgtgcgtac ggtgttcacc gacctcgcca
     3241 acaacctgtt cgcgcacatg ggcctggacg cggtgcgcga cgagacgcgg cgcacgcgcg
     3301 aggcgatgct cgaatccagc ttcacgcgcg ggctgcgcaa cgccatgcag ggctatttcc
     3361 gcagcctgcg tggcaacctg cagcgctcga ccgaggagat cggcgagatc acccgcatgc
     3421 tcgacgccat gtacaagcgc ttcagcgtcg agcacggcct caagctcacc gcacccgaag
     3481 gcttctcgac gctgcgctac gagaaggagc tcgagcgcct ggagacggcc ttcaaccgcc
     3541 agatcaacac cgcgctgacc ctggtcacca ccgagaagca cgcgctcacg cagaagttct
     3601 tcgagaccgt cgcggtgcag gcgcgccaga ccttcgagct cgccaaccgc gacgtcgagc
     3661 agtggctgcg cgcggtgatg tcgccgc
//
LOCUS       DAOQNN010000325         3684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16737_length_3684_cov_8.803527, whole genome shotgun sequence.
ACCESSION   DAOQNN010000325 DAOQNN010000000
VERSION     DAOQNN010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16737_length_3684_cov_8.803527"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKV97_13840"
     CDS             complement(<1..353)
                     /locus_tag="PKV97_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB07010.1"
                     /translation="MLSIYNTLSRSKEAFTPIESGKVRMYVCGMTVYDFCHLGHARVM
                     VVFDMVARWLRASGLDVTYVRNITDIDDKIIRRAQENGESIRQLTDRFIAAMHEDADA
                     LGVLRPDHEPRATDYV"
     gene            646..1233
                     /locus_tag="PKV97_13845"
     CDS             646..1233
                     /locus_tag="PKV97_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB07011.1"
                     /translation="MKRHLLAFATAASLAFPVLAANPTVELKTSQGDIVVEVFADKAP
                     KSAENFVQYVKDGFYDGTVFHRVIDGFMVQGGGFDASMNQKATRAPIENEAKNGLKNE
                     RGTLAMARTSDPHSASAQFFINLVPNTFLDYPSRDGWGYAVFGKVVKGMEVVDKIAKL
                     PTANRGFHQNVPVEAIVVESARVVGTAAPAKKPAK"
     gene            1316..1810
                     /locus_tag="PKV97_13850"
     CDS             1316..1810
                     /locus_tag="PKV97_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB07012.1"
                     /translation="MAVKLHTNHGVITLELDAEKAPVTVANFLAYVEAGHYDNTVFHR
                     VIDGFMIQGGGFEPGMKQKATGEQIKNEADNGLKNDRGTIAMARTQAPHSATAQFFIN
                     VADNAFLNHTSPDLQGWGYCVFGKVSEGMDVVDSIRKVKTGSSGFHQDVPKEDVIIER
                     AEVV"
     gene            1879..2661
                     /locus_tag="PKV97_13855"
     CDS             1879..2661
                     /locus_tag="PKV97_13855"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNB07013.1"
                     /translation="MPEAARAALPALFISDLHLTEDEPANAAAFLDFLHGPARAAASL
                     FILGDLFEYWAGDDDLETPFNARIAAAIRQLADTGTAVFFMTGNRDLLAGPTFAAAIG
                     ATLLEDPARVRCGDRDDADAADAPLLLLAHGDALCTDDLAYQAFRRQVRDPAWQAGFL
                     AQPLAARKAFIAGLRQKSEAAKAEKAMEIMDVNADAVAALLREHGYPTLVHGHTHRPD
                     THHIRVDGHDCIRRVLPDWRGQAAWLAFDGANFTRVDAASGR"
     gene            2774..>3684
                     /locus_tag="PKV97_13860"
     CDS             2774..>3684
                     /locus_tag="PKV97_13860"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNB07014.1"
                     /translation="MNGSEGTIMVLVATRKGAWFFHSDTARRTWRVEGPQLLGQIVSH
                     AVLDPRDGRTLLAAARTGHLGPTLFRSTDLGRHWSEAVQPPAFARAPQGTTGRTVDHT
                     FWLTPAPAGEPGCWYAGTSPQGLFRSTDGGVHWTPFSCLNDDPQYIRWMGTVQDGTPD
                     GPKLHSIIIDPRDPAHLYIGMSGGGVHESRDAGRSFAPLLNGLEVVEGFDRADPSFHD
                     PHCIRMCPSAPDRLYQQNHCGVYRLDRPGDGWVRIGRNMPAEVGDIGFPLVVHPRDAE
                     RAWIFPMDGTDVWPRTSPGGRPGSCAA"
BASE COUNT          589 a         1408 c         1178 g          509 t
ORIGIN      
        1 acgtagtcgg tggcgcgcgg ctcgtggtcg gggcgcagca cgccgagcgc gtcggcgtcc
       61 tcgtgcatcg cggcgatgaa gcggtcggtg agctgacgga tgctctcgcc gttctcctgc
      121 gcgcggcgga tgatcttgtc gtcgatgtcg gtgatgttgc gcacgtaggt cacgtccagc
      181 ccgctcgcgc gcagccagcg cgccaccatg tcgaacacca ccatcacgcg cgcatggcca
      241 aggtggcaga agtcgtagac cgtcatgccg cagacgtaca tgcggacctt gccggattcg
      301 atgggcgtga aggcttcctt gctgcgcgag agggtgttgt agatggaaag catcggtgcg
      361 gacgggtcgt tgctggaggc cggccgccgg cggaacgcgg gcggcggctc gattgtgagg
      421 ggggggggct tgttgctaga atccggcgct cgaggcggac ttcggcaggc ccacgcaagc
      481 gcgcgagtat agcacgctgc ctccgccccc ttccccgggc ccggccgccc ggcgtgcatc
      541 gggagcgcat gcggcacgcg catcgggctt gcccggagcg cccgcgtcct gccagctctc
      601 cagccatccc ggcggccatc cttccgcccc attccaggag cccccatgaa acgacacctg
      661 cttgccttcg ccaccgcggc cagcctagcc ttcccggtgc tcgccgccaa cccgacggtc
      721 gagctcaaga ccagccaggg cgatatcgtc gtcgaggtct tcgccgacaa ggcgcccaag
      781 tccgccgaga acttcgtgca gtatgtgaag gacggcttct acgacggcac cgtgttccac
      841 cgcgtgatcg acggcttcat ggtgcagggc ggcggcttcg acgcaagcat gaaccagaag
      901 gccacgcgcg ccccgatcga gaacgaggcc aagaacggcc tcaagaacga gcgcggcacc
      961 ctggcgatgg cgcgcacctc cgacccgcac tcggcgagcg cgcagttctt catcaacctc
     1021 gtccccaaca ccttcctcga ctacccgtcg cgcgacggct ggggctacgc ggtgttcggc
     1081 aaggtcgtca agggcatgga ggtcgtcgac aagatcgcca agctgcccac cgccaaccgc
     1141 ggcttccacc agaacgtccc ggtcgaggcg atcgtcgtgg agagcgcgcg cgtggtcggc
     1201 accgccgcgc cggcgaagaa gcctgcgaag taagcccgcg aagcaaaccc acaacacccc
     1261 ctgcccggcg gccacgctgc cgggcccaca ccggacccac cccaggagaa cccccatggc
     1321 agtcaagctg cacaccaacc acggcgtcat caccctcgaa ctcgacgccg agaaggcccc
     1381 cgtcaccgtg gccaacttcc tcgcctacgt cgaggccggc cactacgaca acaccgtctt
     1441 ccaccgcgtc atcgacggct tcatgatcca gggcggcggc ttcgagcccg gcatgaagca
     1501 gaaggccact ggcgagcaga tcaagaacga ggccgacaac ggcctcaaga acgaccgcgg
     1561 caccatcgcc atggcgcgca cccaggcccc gcactcggcc accgcgcagt tcttcatcaa
     1621 cgtcgccgac aacgccttcc tcaaccacac ctcgcccgac ctgcagggct ggggctactg
     1681 tgtgttcggc aaggtcagcg agggcatgga cgtggtcgac agcatccgca aggtcaagac
     1741 cggctccagc ggcttccacc aggacgtgcc gaaggaagac gtgatcatcg agcgcgccga
     1801 agtcgtctga tcgtgcccgc gcgcgaccct tcgcaagtcg cctccgcccc ggcccggccg
     1861 ggcggggccg ccgccacgat gccggaagcc gcccgcgcgg cgcttccggc attgttcatt
     1921 tccgacctcc acctgaccga ggacgagccc gccaacgccg ccgccttcct cgacttcctg
     1981 cacggcccgg cgcgcgcggc cgcgagcctc ttcatcctcg gcgacctgtt cgaatactgg
     2041 gccggcgacg acgacctcga gacccccttc aacgcccgca tcgccgcggc gatccgtcag
     2101 ctcgccgaca ccggcaccgc ggtcttcttc atgaccggca accgcgacct gctcgccggc
     2161 ccgaccttcg ccgcggcgat cggcgcgacc ctgctcgagg acccggcgcg cgtgcgctgc
     2221 ggagatcgcg acgacgcaga cgctgccgac gccccgctcc tgctgctcgc ccacggcgac
     2281 gcgctgtgca ccgacgacct cgcctaccag gccttccgcc gccaggtgcg cgaccccgcc
     2341 tggcaggccg gcttcctcgc ccagccgctg gccgcgcgca aggccttcat cgccggcctg
     2401 cgccagaaga gcgaggccgc caaggccgaa aaagccatgg agatcatgga cgtcaacgcc
     2461 gacgcggtcg ccgccctgct gcgcgagcac ggctacccca ccctggtgca cggccacacc
     2521 caccgccccg acacccacca catccgggtg gacggccacg actgcatccg ccgcgtgctg
     2581 cccgactggc gcggccaagc ggcctggctc gccttcgacg gcgcgaattt cacgcgcgtg
     2641 gacgccgctt ccggacgctg atcgatccgc cgcggcgcgc tcctgcatgc ggcaagccgc
     2701 agcatcctgg tctatagaac aggaaaggcg cggcccgggg gcttccgggt ccgcccctgc
     2761 gcggagctca gtcatgaacg gatccgaagg cacgatcatg gtgctggtgg cgacccgcaa
     2821 gggcgcctgg ttcttccaca gcgataccgc gcgccggacc tggcgggtgg aggggcccca
     2881 actgctcggc cagatcgtca gccacgcggt gctcgacccg cgcgacggcc gtacgctgct
     2941 cgccgccgcc aggaccggcc atcttggccc caccttgttc cgctccaccg acctcggccg
     3001 gcactggtcg gaggccgtcc agccgcccgc cttcgcgcgt gccccccaag gcactacagg
     3061 gcgcacggtg gaccacactt tctggctcac ccccgccccc gccggcgaac ccggctgctg
     3121 gtatgcaggc acctcgccgc agggcctgtt ccgctccacc gacggcggcg tgcactggac
     3181 gccgttctcc tgcctcaacg acgacccgca gtacatccgt tggatgggca ccgtgcagga
     3241 cggtacgccg gacgggccca agctgcattc gatcatcatc gacccgcgcg accccgcgca
     3301 tctgtacatc ggcatgtcgg gcggcggcgt gcacgagtcc cgcgacgccg ggcgcagctt
     3361 cgcgccactc ctgaacgggc tcgaggtggt cgagggcttc gaccgcgccg accccagctt
     3421 ccacgacccg cactgcatcc ggatgtgccc gagcgcaccg gaccggctct accagcagaa
     3481 tcactgcggc gtgtaccggc tcgaccgccc gggcgacggg tgggtgcgga tcggtcgcaa
     3541 catgcctgcg gaggtcggcg acatcggctt tccgctcgtg gtgcatccgc gcgacgccga
     3601 gcgcgcgtgg atcttcccga tggacggcac cgacgtctgg ccgcgcacga gcccgggcgg
     3661 ccgcccgggc tcgtgcgcgg ccag
//
LOCUS       DAOQNN010000326         3669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16856_length_3669_cov_11.201992, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000326 DAOQNN010000000
VERSION     DAOQNN010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16856_length_3669_cov_11.201992"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKV97_13865"
     CDS             complement(<1..530)
                     /locus_tag="PKV97_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HNB07015.1"
                     /translation="MEQMSVTQFAGELKMPAAVLLEQLKRAGVEKSGPTDLLTEQDKA
                     RLLDYLRRSHGDTQPKGKITLTRKQTSEIRATDSTGRARTVQVEVRKKRTFVKRDELA
                     EQAGVEAAAVEQAEIAEAVVEAPAPVTEAAAPAVEPAVEAAPVVAAAAPEVVEPPVAE
                     AVAPVAEAVAPVVEPAP"
     gene            complement(544..2019)
                     /gene="nusA"
                     /locus_tag="PKV97_13870"
     CDS             complement(544..2019)
                     /gene="nusA"
                     /locus_tag="PKV97_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNB07016.1"
                     /translation="MSREILLLVDALAREKNVAKDIVFSALETALASATKKRIHDDAD
                     VVVSIDRDSGDYTSKRRWLVMLDEEVANDEAEMGIIDARELRADVQIGDYIEEELEPI
                     DFGRIGAQAAKQVILQKIRDAEREQVLNDFLDRKEFLVSGSIKRMERGNAIIEVGRME
                     AVLPRDQQIPRENLRVGDRVKAFLLRIDRGARGPQLVLSRTAPEFLMKLFELEVPEIE
                     DGLLELKACARDAGLRAKIAVKSNDQRIDPIGTCVGLRGSRVTAVRNEIAGEQIDIIV
                     WSQDPAQFVVAALQPAEVVSIVVDEESHAMDVVVDENNLAIAIGRSGQNVKLASELTG
                     WTINLMSEQESAEKTAQEQQGLRALFMEKLDVDEEVADILIEEGFSSLEEVAYVPLSE
                     MLEIEAFDEDTVNELRNRARNVLLTEAIVTEEQLEKVSDDLLGLEGMDKSLAATLAQQ
                     GIRTRDDLADLAVDELVEMAGIDEERAKALISVARAHWFEE"
     gene            complement(2047..2481)
                     /gene="rimP"
                     /locus_tag="PKV97_13875"
     CDS             complement(2047..2481)
                     /gene="rimP"
                     /locus_tag="PKV97_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNB07017.1"
                     /translation="MRADVEGLIEQVVTGLGYELVDIEFSPKGRLLRVFLDIERGITV
                     DDCATVSNQLQRVFEVENVDYDRLEVSSPGLDRPLKKLADFERFAGEQAQVRLSLPIG
                     NQRNFVGVIERVQDGAVALRTEKGEVLLPFDDIEKARLVPQF"
     gene            complement(2751..>3669)
                     /locus_tag="PKV97_13880"
     CDS             complement(2751..>3669)
                     /locus_tag="PKV97_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNB07018.1"
                     /translation="GDPVRHPDAHRSALGQPEGGERPVRGRGPGGGRNAPTTLSEPER
                     LQKVLAQAGVASRREIEEWVVAGRISVNGLPASLGQKIGPGDRVKVNGKLVPLRFTQR
                     SPRVLIYHKPEGEIVSRDDPEGRPTVFERLPILRKGRWLAVGRLDFNTSGLLLFTNDG
                     DLANKLMHPRYELEREYAVRILGELTEEQVKSLTDGIQLEDGPAKFNLLRDEGGEGAN
                     HWYRVTISEGRNREVRRMFEAVGLTVSRLMRVRYGSVELPARLKRGMWMEMPEAEACA
                     LAGLPPPQQSRQQDLRDKRPVKLHRTQAR"
BASE COUNT          565 a         1263 c         1207 g          634 t
ORIGIN      
        1 ggggccggct cgacgaccgg agctacggcc tcggcgacgg gcgcgacggc ctcggccacc
       61 ggcggctcga ccacctccgg cgcggcggct gcaacaaccg gcgccgcttc gacggcaggc
      121 tcgaccgcgg gcgcagcggc ctcggtgacc ggcgcgggcg cctcgacgac cgcctcggcg
      181 atttcggctt gctcgaccgc cgcagcctcg accccggcct gctcggcgag ctcgtcacgc
      241 ttgacgaagg tgcgcttctt gcgcacctcg acctgcaccg tgcgcgcacg cccggtggag
      301 tcggtggcgc ggatctcgga ggtctgcttg cgcgtcaggg tgatcttgcc cttgggctgg
      361 gtgtcgccgt gcgaacgccg caggtagtcc agcagcctcg ccttgtcctg ttcggtgagc
      421 aggtcggtgg ggccggattt ttccacgccc gcacgcttca actgctcgag cagcaccgcg
      481 gccggcattt tcagttcgcc ggcaaactgg gtcacgctca tctgttccat tactgccctc
      541 ccatcattct tcgaaccaat gggcgcgcgc aacggaaatc agcgccttgg ctctttcttc
      601 gtcgatcccg gccatttcga ccagctcgtc gaccgcaagg tcggccaggt cgtcgcgggt
      661 acgaatgccc tgctgggcca gtgtggcggc cagcgacttg tccatgcctt cgaggccgag
      721 caagtcgtcg gaaaccttct cgagctgctc ctcggtgacg atggcctcgg tcagcagcac
      781 gttgcgcgct cgattgcgca gttcgttgac cgtgtcctcg tcgaaggcct cgatctcgag
      841 catttcggag agcggcacgt aggccacctc ttcgagcgag gagaaaccct cctcgatcag
      901 gatgtcggcg acttcctcgt cgacgtccag tttttccatg aacagcgcgc gcaggccctg
      961 ctgctcctgg gcggtctttt cggccgactc ctgctcgctc atcaggttga tcgtccaccc
     1021 ggtgagctcg gaggcgagct tgacgttctg gccgctgcgg ccgatggcga tcgcgaggtt
     1081 gttctcgtcg accaccacgt ccatcgcgtg cgactcctcg tccacgacga tggagacgac
     1141 ctcggcgggc tgcagcgcgg cgaccacgaa ctgggcggga tcctgcgacc acacgatgat
     1201 gtcgatctgc tcgccggcga tctcgttgcg cacggcggtg acgcgcgagc cgcgcaggcc
     1261 gacgcaggta ccgatcgggt cgatgcgttg gtcgttggac ttgaccgcga tcttggcgcg
     1321 caggccggcg tcgcgggcgc aggccttgag ctcgagcagg ccgtcctcga tctcggggac
     1381 ctcgagctcg aagagcttca tgaggaattc gggcgcggtg cgcgacagca ccagctgcgg
     1441 gccgcgcgcg ccgcggtcga tgcgcagcag gaaggccttg acacgatcgc ccacgcgcag
     1501 attctcgcgc gggatctgct ggtcgcgcgg cagcacggct tccatgcggc cgacctcgat
     1561 gatcgcgttg ccgcgctcca tgcgcttgat cgagccggag acgaggaact ccttgcggtc
     1621 gaggaagtcg ttgagcacct gctcgcgctc ggcgtcgcgg atcttctgca ggatgacctg
     1681 cttggcggcc tgggcgccga tgcgaccgaa gtcgatcggc tcgagctctt cctcgatgta
     1741 gtcaccgatc tgcacgtcgg cgcgcagctc gcgggcatcg atgatgccca tctcggcctc
     1801 gtcgttcgcg acctcctcgt cgagcatcac cagccagcgg cgcttcgagg tgtagtcgcc
     1861 ggagtcgcgg tcgatcgaca ccaccacgtc ggcatcgtcg tggatgcgtt tcttggtcgc
     1921 agaggccaag gcggtttcga gcgcggaaaa aacgatgtcc ttggcgacgt tcttctcgcg
     1981 cgccaaggca tcgacaagca gcaggatctc gcggctcatt tcagtaaaac ctccacatcc
     2041 acagtctcaa aattggggca ccaggcgcgc tttctcgatg tcgtcgaagg gcagcagcac
     2101 ctcgcccttc tcggtgcgca gcgccaccgc gccgtcctgc acccgctcga tcacgcccac
     2161 gaaattgcgt tgattgccga tcggcagcga caggcggacc tgcgcctgct cgccggcgaa
     2221 acgttcgaaa tcggccagct tcttcaacgg gcggtcgagg ccgggtgagg acacctcgag
     2281 ccggtcgtaa tcgacgttct cgacctcgaa cacgcgctgc agctggttgc tgacggtggc
     2341 gcaatcgtcg accgtgatgc cgcgctcgat gtcgaggaag acgcgcagca agcggccctt
     2401 gggcgagaac tcgatatcga cgagttcgta tcccagtccg gtcaccacct gctcgatcaa
     2461 tccctcgacg tccgcgcgca tctgcctgcc ccaccgcttc ggcgccacgt cgggcgccac
     2521 aaataaaaaa tgggcgaaac gcccatcaaa caattgttcc tgcacgaaaa accgctggcc
     2581 acaaccgcat gacggagcga acgcctgccg tggctcgtgg ccacaaagga aatccatcca
     2641 tcaaagccga acgagtatac agccaaaccg cggggacgag caaacgcacg cgcgaagatc
     2701 gcgcgtgcgc cactcccccg gcgaggactg cggagtccct gcgggacgcc tcaacgcgcc
     2761 tgggtgcggt gcagcttcac cggccgcttg tcgcgcagat cctgctgacg gctctgctgc
     2821 ggcggcggca ggccggcgag cgcgcaggcc tcggcctcgg gcatctccat ccacatgccg
     2881 cgcttgaggc gcgcgggcag ctcgacgctg ccgtagcgca cccgcatcag ccggctcacg
     2941 gtcaggccca cggcttcgaa catgcgccgc acttcgcggt tgcggccctc cgagatcgtc
     3001 acccggtacc agtggttggc accctcgccg ccctcgtcgc gcagcaggtt gaacttggcc
     3061 gggccgtcct cgagctggat gccgtcggta agcgacttca cctgctcttc ggtgagctcg
     3121 ccgaggatgc gcaccgcgta ctcgcgctcg agttcgtagc gcgggtgcat cagcttgttg
     3181 gcgaggtcgc cgtcgttggt gaacaacagc agccccgagg tgttgaagtc caggcgcccg
     3241 accgccagcc agcgcccctt gcgcaggatg ggcaggcgct cgaatacggt gggacggccc
     3301 tcggggtcgt cgcgcgagac gatctcgcct tcgggcttgt ggtagatcag cacgcgcggc
     3361 gagcgctggg tgaagcgcag cggcacgagc ttgccattga ccttgacgcg gtcgcccggg
     3421 ccgatcttct ggccgagcga ggccggcagg ccattgaccg agatgcgccc ggccaccacc
     3481 cattcctcga tctcgcgccg cgaggcgacg cccgcctggg cgagcacctt ctgcaggcgc
     3541 tcgggctcgc tgagcgtggt gggcgcgttg cgcccgccgc ccggcccacg gccacgcacg
     3601 gggcgctcgc cgccctcggg ctggccgagc gcggaacggt gagcgtcggg atggcggacg
     3661 ggatcgccc
//
LOCUS       DAOQNN010000327         3669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16857_length_3669_cov_7.846154, whole genome shotgun sequence.
ACCESSION   DAOQNN010000327 DAOQNN010000000
VERSION     DAOQNN010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16857_length_3669_cov_7.846154"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..457)
                     /gene="fliO"
                     /locus_tag="PKV97_13885"
     CDS             complement(95..457)
                     /gene="fliO"
                     /locus_tag="PKV97_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNB07019.1"
                     /translation="MPELGASLVQMSFGLAVVVGLLFACLWLLRRLSAPRGGGAAIKV
                     LGAAAVGPRERVVLVEIGEDVLVLGVAPGSVARLHELKRVDLPLPADLPAQPLPGGKT
                     FATWLRQAAQRRERRDEA"
     gene            complement(466..933)
                     /gene="fliN"
                     /locus_tag="PKV97_13890"
     CDS             complement(466..933)
                     /gene="fliN"
                     /locus_tag="PKV97_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNB07020.1"
                     /translation="MADENLITDDDWAAAMQEQAAAEEGGDSEADRVAAELAAAAMSD
                     SPYQARPASQLFEDFGAPAGKPGALNDFDMILDIPVQITVELGRTKLSIRNLLQLAHG
                     SVVELDGLAGEPMDVLVNGTLIAQGEVVVVNDKFGIRLTDIITPAERMRKIRN"
     gene            complement(926..1936)
                     /gene="fliM"
                     /locus_tag="PKV97_13895"
     CDS             complement(926..1936)
                     /gene="fliM"
                     /locus_tag="PKV97_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNB07021.1"
                     /translation="MSADFLSQEEVDALLRGVTGESDEPVADEAGVEGVRPYNLATQE
                     RIVRGRMPTLELINERFARYLRIGLFNYMHRNAEVSAGPIKVQKYAEFVRNLVVPTNL
                     NLITAKPLRGTGLVVFDPNLVFLVVDNMFGGDGRFHTRVEGRDFTPTEQRIIRGMLDV
                     VFAEYVRAWAPVFNLQPEFIRSEMNSQFANIATPSEVVVSTSLSVEFGGSQAEMHICF
                     PYSMLEPIRDLLYSSMQSDHLTSDRRWIKLLTRQLQTAEVELACSFGTARVSLRDIVD
                     MRVGDVIPLAVQPVLQAEVDGVAVFECRQGTRNGRYALRVERFITAEDNEPPMIPGAQ
                     NG"
     gene            complement(1949..2521)
                     /locus_tag="PKV97_13900"
     CDS             complement(1949..2521)
                     /locus_tag="PKV97_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNB07022.1"
                     /translation="MAKAPAKTEEGDETPKKKGKLGLILIIVLVVIVLVLGALVALLL
                     LKGGKDKEHEEPAAHAAPAAQQLHTTGTVDLTKPPTFIPLDAFTVNLRRDEGDHYLQV
                     VAVMRVADATMDPTLKAFMPEIRHRINLLLSSKLPSEVATVEGREALSLAITESVNEA
                     LGFKPVRDAAGKPVPNGPVQAVLFTSFIIQ"
     gene            complement(2615..>3669)
                     /locus_tag="PKV97_13905"
     CDS             complement(2615..>3669)
                     /locus_tag="PKV97_13905"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNB07023.1"
                     /translation="HGWFAQLVAHAPLPLLERLGLPAAPELVEDTEVLKADLMRRFHA
                     AVDADATSADTVTATASSPTDPAALAGLPAAIAALLAGRAEAPSGADATGSEAGTLQL
                     VADGGRKSLQNSALLPKTDLSATAGGNTGPDGNATPAFLLQGREFAAATMAARGASGA
                     GAGGTAADGLEVPGTSNANGNLPAGLAHTPGATHLLRQASSAHAASPQLPVATPAGEQ
                     GWAEDVGNQVRWMLGRAESKAELVLTPPNMGKLEVSINLAGDQTTAQFIASSQAARDA
                     LERALPQLREALQQAGILLGDANVSTSGQGREGDGREGSGGRGERGEAGASGHAGTGG
                     AGVWLKQQQGMVDTFA"
BASE COUNT          512 a         1262 c         1319 g          576 t
ORIGIN      
        1 cagggcctgc gccgtcgcca gcaggggcag gccggcgagc gcgcaggcga gcgcgaggcg
       61 cagcagtgcc acggggtggc tcggggcgcg gcgctcaggc ttcatcgcgg cgttccctgc
      121 gttgcgcagc ctggcgcagc caggtggcga aggtcttgcc gcccggcagg ggctgggccg
      181 gcaggtcggc gggcagcggc aggtcgacgc gcttgagttc gtgcaggcgc gcgacgctgc
      241 cgggcgccac gcccagcacc agcacgtctt caccgatctc gaccagcacc acgcgctcgc
      301 gcgggccgac cgcggccgcg ccgagcacct tgatcgccgc gccgccgccg cgcggggcgg
      361 acaggcggcg gagcagccac aggcaggcga acagcaggcc gaccaccacc gccaggccga
      421 agctcatctg cacgagactg gcgccgagtt cgggcatcgc gctgctcagt tgcggatctt
      481 ccgcatgcgc tcggcggggg tgatgatgtc ggtgaggcgg atgccgaact tgtcgttgac
      541 caccacgacc tcgccctggg cgatcagggt gccgttcacc agcacatcca tgggctcgcc
      601 ggccaggccg tcgagctcga ccaccgagcc gtgcgcgagc tgcagcaggt tacggatcga
      661 cagcttggtg cgcccgagct ccacggtgat ctgcaccggg atgtcgagga tcatgtcgaa
      721 gtcgttgagc gcgcccggct tgcccgccgg ggcgccgaag tcctcgaaga gctggctggc
      781 ggggcgggcc tggtagggcg agtcgctcat cgccgcggcc gcgagctcgg cggcgacccg
      841 atcggcttcg ctgtcgccgc cttcctcggc ggcggcctgt tcctgcatcg cggctgccca
      901 gtcgtcgtcg gtaatcaggt tctcgtcagc cattctgggc tcccggaatc atggggggtt
      961 cgttgtcctc ggcggtgatg aagcgctcca cgcgcagcgc atagcgcccg ttgcgcgtgc
     1021 cctggcggca ttcgaacacc gccacgccgt cgacctcggc ctgcagcacc ggctgcaccg
     1081 cgagcgggat cacgtcaccg acgcgcatgt cgacgatgtc gcgcaggctc acccgcgccg
     1141 tgccgaacga gcatgcgagt tccacctcgg cggtctgcaa ctgacgcgtc agcagcttga
     1201 tccagcgccg gtccgaggtg aggtggtcgc tctgcatcga ggaatagagc aggtcgcgga
     1261 tcggctccag catcgagtac gggaagcaga tgtgcatctc cgcctgcgag ccgccgaact
     1321 cgaccgagag gctggtcgac accaccacct cggagggcgt cgcgatgttg gcgaactgcg
     1381 agttcatctc cgagcggatg aactcgggct gcagattgaa caccggtgcc caggcgcgca
     1441 cgtattcggc aaacacgacg tcgagcatgc cgcggatgat gcgctgctcg gtcggggtga
     1501 aatcgcggcc ctccacgcgg gtgtggaagc gcccgtcgcc gccgaacatg ttgtccacca
     1561 ccaggaagac caggttgggg tcgaacacga ccagcccggt gccgcgcagc ggcttggcag
     1621 tgatcaggtt gagattggtc ggcaccacca ggttgcgcac gaactcggcg tacttctgca
     1681 ccttgatcgg ccccgccgac acctcggcgt tgcggtgcat gtagttgaac aggccgatgc
     1741 gcaggtagcg cgcgaagcgc tcgttgatga gctcgagcgt gggcatgcgc ccgcgcacga
     1801 tgcgttcctg ggtggcgagg ttgtaggggc gtacgccctc gacgccggct tcgtccgcga
     1861 ccggctcgtc cgactcgccg gtgacgcccc gcagcagggc gtcgacctct tcctgggaga
     1921 gaaagtccgc ggacatggcg gcgatccctt actggatgat gaaggaggtg aacagcacgg
     1981 cctgcaccgg gccgttgggc accggcttgc cggccgcgtc gcgcaccggc ttgaagccga
     2041 gcgcctcgtt cacgctctcg gtgatcgcca gcgacaaggc ctcgcggccc tcgaccgtgg
     2101 ccacctcgga cggcagcttg ctcgacagca gcaggttgat gcggtggcgg atctccggca
     2161 tgaaggcctt cagcgtcggg tccatggtcg cgtcggccac gcgcatcacg gccaccacct
     2221 gcaggtagtg gtcgccctcg tcgcggcgca ggttgaccgt gaaggcgtcg agcgggatga
     2281 aggtgggcgg cttggtgagg tccaccgtgc cggtggtgtg cagctgctgc gcagccggcg
     2341 cggcgtgggc cgccggctcc tcgtgctcct tgtccttgcc gcccttgagc agcagcagcg
     2401 cgacgagcgc gccgagcacg agcacgatca ccacgagcac gatgatgagg atcaggccga
     2461 gcttgccctt cttcttgggg gtctcgtcgc cttcctcggt ctttgccggc gccttggcca
     2521 tgcgaatctc ctgtagtacg gattgaatta tccgggaggt ccgcacccgc caaagcgccg
     2581 attagtgggg caagcccgcc ccaattcgcg cgcttcaggc gaaggtgtcg accatcccct
     2641 gttgctgttt cagccacacc ccggcaccgc cggtgccggc gtggccactc gcgcccgcct
     2701 cgccgcgttc gccacgaccg ccgctgcctt cgcgtccatc gccctcgcgg ccctggccgg
     2761 aggtgctcac gttggcgtcg cccagcagga tgccggcctg ctgcagcgcc tcgcgcagct
     2821 gcggcagcgc gcgttcgagc gcgtcgcgcg cggcctggct cgaggcgatg aactgcgccg
     2881 tcgtctggtc gccggcgagg ttgatcgaca cctcgagctt gcccatgttc ggcggcgtca
     2941 gcacgagttc ggccttgctc tcggcacggc cgagcatcca gcgcacctga ttgccgacgt
     3001 cttccgccca gccctgctcg ccggccgggg tcgccaccgg cagctgcggc gatgcggcgt
     3061 gcgcggagga cgcctggcgc agcagatgtg tcgcgccagg cgtgtgcgcc aggccggccg
     3121 gcaggttgcc gttcgcgttg ctcgtgcccg gcacctcgag gccatccgcc gcggtgccgc
     3181 ctgcaccggc gccgctcgcg ccgcgagccg ccatggttgc ggcggcgaac tcgcggccct
     3241 ggagcaggaa ggccggcgtg gcgttgccat cgggaccggt gttgccgcca gccgtggccg
     3301 acagatcggt tttcggcagc agtgcggaat tctgaagcga cttgcgcccg ccgtccgcaa
     3361 ccagctgcag cgtgcccgct tcggagccgg tcgcatccgc tccggagggg gcttcggcgc
     3421 gaccggcgag cagtgcggca atcgctgccg gcaggccggc aagggctgcc gggtcggtgg
     3481 gcgagcttgc cgtcgcggtg accgtatcgg cagatgttgc gtcggcgtcg accgcggcgt
     3541 ggaagcggcg catcaggtcg gccttgagga cctcggtgtc ctcgaccagt tcgggcgccg
     3601 cgggcagacc caggcgctcc agcagcggca gtggcgcgtg ggcgacgagc tgcgcgaacc
     3661 agccgtgga
//
LOCUS       DAOQNN010000328         3665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16891_length_3665_cov_9.014127, whole genome shotgun sequence.
ACCESSION   DAOQNN010000328 DAOQNN010000000
VERSION     DAOQNN010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16891_length_3665_cov_9.014127"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PKV97_13910"
     CDS             complement(<1..1488)
                     /locus_tag="PKV97_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukotoxin LktA family filamentous adhesin"
                     /protein_id="HNB07024.1"
                     /translation="MHHHREPEPVAAAPRLKEIVVHVTCALIVSHGLVPELAWAVDIV
                     PDGRTATSVSPLGTNAADVRNTDRLLGNGNAVNSFSRFNVGSGETANLHLPDGAAALI
                     NLVRDQKTTIDGMLNAIKEGRIGGKVYFANPHGVVVGPSGKVNVGSLHVSTPSQRFVD
                     DFFDAPGGAEASAAALVEGRAPRNTAAEIRIDGEVNATDQVGLSAGSITVGGKVLSGA
                     KFAGGAPDFSDVVNVHGLATGGAVVENQGRIEIVGDSGIELTGTVSSRRVNVGDDASL
                     RSGVSIAASGDIALRAPTIRIGSGGKLLAAGSDTHAGGDVTLQAQAVGGTLDALSDTA
                     ARIEASGATLTGRDLHLQASAEHRSTLAPLVTKTTSATIEVDGSTLEASGRATLEATA
                     TAAIATPDVVPLAVVDVDVAASVRVQGDTRITSAGPARLGATTTTTIDARPGLPDLGA
                     LPADAGAAIVIADTTASVDLLGNAELASTADKTELAATNRTTVDAA"
     gene            complement(1706..2485)
                     /locus_tag="PKV97_13915"
     CDS             complement(1706..2485)
                     /locus_tag="PKV97_13915"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNB07025.1"
                     /translation="MRYQLELFFTALGFFTRLPVPAWVPWSSERMHHAARYFALVGWV
                     VGGAGALGYLALAWLLPPAVAVVLSMALTIRLTGAFHEDGFADSCDGLGGGWDKAQIL
                     AIMKDSRIGSYGTIGMVLMLLAKAAALVELADPASPGGSVALALLVAHPLSRLAATVV
                     LHRLPYVRADDSAKSVAVARPLAPAGLALAVASGLLPLLLLPPAAALCALLAAAAVTT
                     WSARLFKRRLGGYTGDLLGATQQASELAIYLALLAASRLAS"
     gene            complement(2485..3546)
                     /gene="cobT"
                     /locus_tag="PKV97_13920"
     CDS             complement(2485..3546)
                     /gene="cobT"
                     /locus_tag="PKV97_13920"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNB07026.1"
                     /translation="MKPYAIASPDPLLSAAIQHKIDRKTKPRGALGRLEALAHQIALL
                     QQTLEPELRQPHIVVFAGDHGAARAGVSAYPQDVTWQMVENFLAGGAAINVFTRQLGL
                     DLTVVDAGVNHDFGKRPGLIDAKIAPGTANYIEEPAMSAAQRDAALERGRGVAHALAD
                     NGCNCIGLGEMGIGNTASASLLTHCLVGAEHDAELYTVTGRGTGLDDPGLFRKRSMLA
                     QALQRGGRPHDPLEVLVQYGGLEIAMMAGAILGAAERRMLVLVDGFIVTSAVLVAHAL
                     APAVLSCCVFAHRSQEPGHRVQLDFLQAEPLMELDLRLGEGTGAALAFPLVQAAVNFI
                     NEMASFESAGVSDQGRSPG"
BASE COUNT          483 a         1205 c         1435 g          542 t
ORIGIN      
        1 ggccgcgtcg accgtcgtgc ggttggtcgc ggccagttca gtcttgtccg ccgtgctggc
       61 gagctcggcg ttgccgagca gatccacgct ggcggtggtg tcggcgatga cgatcgccgc
      121 gccggcgtcg gccggcagcg cgccgaggtc gggcaggccg ggacgggcgt cgatggtcgt
      181 ggtggtggtg gcgccgaggc gggcgggacc ggcgctggtg atccgggtgt cgccctggac
      241 gcgcacgctc gccgcgacgt ccacatcgac caccgccagc ggcacgacat ccggggtggc
      301 gatggcggcg gtggcggtgg cctcgagggt ggcgcggccg ctcgcctcca gcgtggatcc
      361 gtccacctcg atcgtcgcgc tcgtggtctt ggtgaccagc ggcgccagcg tgctacggtg
      421 ttccgcgctc gcctgcaggt gcaggtcgcg gccggtgagg gtcgcgccgc tcgcctcgat
      481 gcgcgccgcg gtgtcggaca aggcgtcgag cgtgccgccg acggcctgcg cctgcagcgt
      541 cacgtctccg cccgcgtggg tgtcgctgcc cgcggcgagc agcttgccgc cgctgccgat
      601 gcggatcgtg ggcgcacgca gggcgatgtc gcccgaggcg gcgatcgaca cgccgctgcg
      661 caggctggcg tcgtcgccga cgttcacccg gcgcgaggag accgtgccgg tgagctcgat
      721 gccgctgtcg ccgacgattt cgatgcggcc ctggttctcg accaccgcgc cgcccgtcgc
      781 caggccgtgc acgttgacca cgtcgctgaa gtcgggggcg ccgccggcga acttcgcgcc
      841 gctcagcacc ttgccgccca ccgtgatcga gcctgcggac aggccgacct ggtcggtggc
      901 gttgacctcg ccgtcgatgc ggatctcggc cgcggtgttg cgcggcgcgc ggccctcgac
      961 cagggcggcg gccgaggcct cggcgccgcc cggggcgtcg aagaagtcgt cgacgaagcg
     1021 ctggctgggc gtggacacgt gcaggctgcc gacgttgacc ttgccgctcg ggccgacgac
     1081 gacgccgtgc gggttggcga aatagacctt gccgccgatc cggccttcct tgatggcatt
     1141 gagcatgccg tcgatggtgg tcttctggtc gcgcaccaga ttgatgagcg cggcggcgcc
     1201 gtcgggcagg tggaggttgg cggtctcgcc gctgccgaca ttgaagcggc tgaaggagtt
     1261 gaccgcattg ccgttgccga ggaggcggtc ggtgttgcgg acgtcggcgg cgttcgtgcc
     1321 gagcgggctc acgctggtcg cggtgcggcc gtcggggacg atgtcgaccg cccacgccag
     1381 ctcgggcacc aggccgtggc tgacgatcag cgcgcaagtg acatggacga cgatttcctt
     1441 caggcgcggc gcagctgcga ccggctcggg ctcgcgatgg tgatgcatgg cttgtctccc
     1501 cctgcccctc ttgtcgaggg cgcggagccg cgcttcaggt cggggctcca ccatgctttc
     1561 cgcgcacgcc tgaagacgtg tccgacttgc cggagaaaga ctccagatca actcatattg
     1621 ggttgccgac ttgtcgggag caagtttttt ttgtcctggg tcaagatccg cccaggccgc
     1681 atggggattg cacggcggtg caggctcagc tcgccagccg cgatgcggcg agcagggcga
     1741 ggtagatggc gagctcgctc gcctgctggg tggcgccgag caggtcgccg gtgtagccgc
     1801 cgagccggcg cttgaagagg cgcgcgctcc acgtcgtcac cgcagcggcg gcgagcaggg
     1861 cgcagagcgc ggcggcgggt ggcagcagca gcaggggtag caggccgctg gcgacggcca
     1921 gcgcgagccc ggccggtgcc agcgggcgcg cgacggcgac cgacttggcc gaatcgtcgg
     1981 cgcgtacgta gggcaggcgg tgcagcacca cggtggcggc caggcgcgac agcggatggg
     2041 cgacgagcag ggcgagcgcc acgctccccc ccgggctcgc cgggtcggcc agttccacca
     2101 gcgccgccgc cttggcgagc agcatcagca ccatgccgat ggtgccgtag ctgccgatcc
     2161 gcgagtcctt catgatcgcc aggatctgtg ccttgtccca gccgccgccg aggccgtcgc
     2221 agctgtcggc gaagccgtcc tcgtggaagg cgccggtgag gcggatggtg agcgccatcg
     2281 acaggacgac cgccacggcg ggcggcagca gccaggcgag ggcgaggtag ccgagcgcgc
     2341 ctgcgccgcc caccacccag cccacgagtg cgaagtagcg cgccgcgtgg tgcatgcgct
     2401 cggacgacca cggcacccat gcgggcaccg ggaggcgggt gaagaagccg agcgcggtga
     2461 agaagagttc gagctggtag cgcatcagcc cgggctgcgg ccctggtcgc tgaccccggc
     2521 ggactcgaag ctcgccatct cgttgatgaa attcaccgcc gcctgcacga gcgggaaggc
     2581 gagtgccgcg ccggtgcctt cgcccaggcg gaggtcgagt tccatcagcg gttcggcctg
     2641 caggaagtcg agctgcacgc ggtggccggg ctcctgcgag cgatgcgcga agacgcagca
     2701 cgacagcacc gcgggtgcca gggcgtgggc gacgagcacg gccgaggtga cgatgaagcc
     2761 gtcgaccagc accagcatgc ggcgctcggc ggcgccgagg atggcgcccg ccatcatggc
     2821 gatctcgagg ccgccatact gcaccagcac ctccagcggg tcgtgtggtc gaccaccgcg
     2881 ctgcagcgcc tgggcgagca tgctgcgctt gcggaacagg ccggggtcgt ccaggccggt
     2941 gccgcggccg gtgacggtat agagctcggc gtcgtgctcg gcgcccacca ggcagtgggt
     3001 gagcagcgag gccgacgcgg tgttgccgat acccatctcg cccaggccga tgcagttgca
     3061 gccgttgtcg gcgagcgcat gggcgacgcc gcgcccgcgc tcgagcgctg cgtcgcgctg
     3121 cgcggcgctc atggccggct cttcgatgta gttggccgtg ccgggcgcga tcttggcgtc
     3181 gatcagcccc gggcgcttgc cgaagtcgtg gttgacgccg gcatcgacca cggtgagatc
     3241 cagcccgagc tggcgggtga atacgttgat cgccgcgccg ccggcgagga agttctccac
     3301 catctgccag gtcacgtcct gcgggtaggc ggacacgccg gcacgtgccg cgccgtggtc
     3361 gccggcgaag acgacgatgt gcggctggcg cagttcgggt tccagggtct gctgcagcag
     3421 cgcgatctgg tgcgcgagcg cctccaggcg gccgagcgcg ccgcgcggct tggtcttgcg
     3481 gtcgatcttg tgctggatcg cggcggagag cagcgggtcg ggcgaggcga tggcgtaggg
     3541 cttcatgcgg caggtggcga gggtggggtt gtgggcgccg gggcggaggg ttccgcgtcc
     3601 ggcaggtcgc gatagaggtc tgcgagggtg agctcgaagt cgaaggcggg cagggcgagg
     3661 cgggc
//
LOCUS       DAOQNN010000329         3654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16977_length_3654_cov_11.165324, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000329 DAOQNN010000000
VERSION     DAOQNN010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16977_length_3654_cov_11.165324"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PKV97_13925"
     CDS             <1..1416
                     /locus_tag="PKV97_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HNB07027.1"
                     /translation="DNRPEAAAKAVAGIRAQFAKLADKGRMGAEAAAAASERLVPVEQ
                     LADLADAALVVEAIVESLEAKQKLYRDLEEIVGADCIFGTNTSSISVTAIGSALQRPE
                     RLAGLHFFNPAPLMKLVEIVSGLATARVVADTLFATAAAWGKTPVHARSTPGFIVNRV
                     ARPYYAEALRILNEGGGDCATLDAIMRDAGGFRMGPFELMDMIGHDVNFAVTRSVWNA
                     FYNDARFTPSLIQQELVDAGFFGRKSGRGFYDYREGAARPAPQVAVAQHAPDEIVLHG
                     HSAAVHALADRLFERNIPHTWGEANDGRIAETGGAVIFVTDGRTATQRATEMGIANVV
                     LIDLALDYATAGRIAVGAAINCFAPAVGAAISLLQAAGFEVCRLGDTPGLAVMRTVAM
                     LANEAADAVNQGVCDAAGADAAMKLGVNYPRGPLEWADRVGLPAICAVLANLARFYGE
                     DRYRVSPLIQQRVFAGKKIHD"
     gene            1409..1876
                     /gene="paaI"
                     /locus_tag="PKV97_13930"
     CDS             1409..1876
                     /gene="paaI"
                     /locus_tag="PKV97_13930"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HNB07028.1"
                     /translation="MTDAQPIPTDPQALAEAVTAAMWSRDRTAQALGMRIEHVGPGRS
                     RVSMTVRGDMVNGHHICHGGLIFTLADTAFAYACNAYNRNTVASGCNIDFVATGREGD
                     TLQAEAVERTLAGRTGVYDVTVRDSAGNTIALFRGKSYRISGEVIAGLAAENA"
     gene            1928..3253
                     /gene="paaF"
                     /locus_tag="PKV97_13935"
     CDS             1928..3253
                     /gene="paaF"
                     /locus_tag="PKV97_13935"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNB07029.1"
                     /translation="MPVKSPRPGDLDPIETASRDEISALQLQRLQWSVRHTYDNVAPY
                     RKRCEEAGVHPDDLQSLADLAKFPFMTKTDLRDNYPFGLFAVPRDKVARLHASSGTTG
                     KSVVVGYTKRDLDNWADVVARSLRAAGVRAGDMVHNAYGYGMFTGGLGAHYGIERAGC
                     TAVPMSGGQTEKQVQQIMDFRPDAIMVTPSYSLVIAEEFERLNIKPEDISLKIGIFGA
                     EPWGEGMRSEIERKLGIDAIDIYGLTEVMGPGVASECIETKDGPVIWEDHFYPEIIDP
                     DTGEVLPDGEDGELVFTSLTKEAFPVIRYRTRDLTRLLPPTARSFRRIGKITGRSDDM
                     LIVRGVNVFPTQIEEQIMRDRRLSGNYQIVLTRDGHLDNVEIRVEVQRELSGKLSRTE
                     TENIARELQHHIKTIIGISTKVNVMEFEAIPRTLTGKARRVIDQRPKQL"
     gene            3264..>3654
                     /locus_tag="PKV97_13940"
     CDS             3264..>3654
                     /locus_tag="PKV97_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNB07030.1"
                     /translation="MTQAFICDAIRTPIGRYGGTLASVRADDLGAVPLKALMTRNPQV
                     DWTAVEDIIYGCANQAGEDNRNVARMSGLLAGLPIEVPGTTVNRLCGSGMDAIGLAAR
                     SIKSGETELMIAGGVESMSRAPFVMGKA"
BASE COUNT          619 a         1377 c         1157 g          501 t
ORIGIN      
        1 gacaaccgcc ccgaggccgc cgccaaggcc gtcgccggca tccgcgccca gttcgccaag
       61 ctcgccgaca aggggcgcat gggcgccgag gccgccgccg cggccagcga gcgtctggtg
      121 ccggtcgagc agctcgccga tctggccgac gcggccctcg tcgtcgaggc catcgtcgag
      181 agcctcgagg ccaagcagaa gctctaccgc gacctcgaag aaatcgtggg cgccgactgc
      241 atcttcggca ccaacacctc gtcgatctcc gtcactgcga tcggctcggc cctgcagcgc
      301 cccgagcgcc tggccggcct gcacttcttc aacccggcgc cgctgatgaa gctggtcgag
      361 atcgtctccg gcctcgccac cgcgcgcgtg gtcgccgaca ccctgttcgc caccgccgcg
      421 gcctggggca agacgccggt gcatgcgcgc tcgacgccgg gcttcatcgt caaccgcgtc
      481 gcccgtccct actacgccga ggcgctgcgc atcctcaacg aaggcggtgg cgactgcgcc
      541 acactcgacg cgatcatgcg cgacgccggc ggtttccgca tggggccgtt cgagctgatg
      601 gacatgatcg gccacgacgt caatttcgcc gtcacacgct cggtgtggaa cgccttctac
      661 aacgatgcgc gcttcacgcc ctcgctgatc cagcaggagc tggtcgacgc cggctttttc
      721 ggtcgcaagt cgggccgcgg cttctacgac taccgcgagg gcgccgcgcg ccccgcaccc
      781 caggtcgcgg tggcgcagca cgcaccggac gagatcgtgc tgcacggcca ttcggccgcc
      841 gtgcatgcgc tcgccgaccg cctcttcgag cgcaacatcc cccatacctg gggcgaagcc
      901 aacgacggtc gcatcgccga gacggggggc gcggtgatct tcgtcaccga cggccgcacc
      961 gccacgcaac gcgccaccga gatgggcatc gccaacgtgg tgctgatcga cctcgcactg
     1021 gactacgcca ccgccggccg catcgcggtc ggcgccgcga tcaactgctt cgcgcccgcg
     1081 gtcggcgccg cgatcagcct gctgcaggcg gccggcttcg aggtctgccg cctgggcgac
     1141 accccgggac tggcagtgat gcgcaccgtc gcgatgctcg ccaacgaagc ggccgacgcg
     1201 gtcaaccagg gggtgtgcga tgccgccggc gccgatgcgg cgatgaagct cggcgtcaat
     1261 tacccgcgcg gaccgctgga atgggccgac cgcgtcggcc tgccggcgat ctgcgccgta
     1321 ctcgccaacc tcgcccgctt ctacggcgag gaccgctatc gcgtgtcccc gctgatccag
     1381 cagcgcgtgt tcgccggaaa gaagatccat gactgatgcg cagcccatcc cgaccgatcc
     1441 gcaggccctg gccgaggccg tgaccgcggc gatgtggtcg cgcgaccgta ccgcccaggc
     1501 gctcggcatg cgcatcgagc acgtcggtcc cggccgctcc agggtcagca tgacggtgcg
     1561 cggcgacatg gtgaacggcc accacatctg ccacggcggc ctcatcttca ccctggccga
     1621 caccgccttc gcctacgcct gcaacgccta caaccgcaac accgtggcgt ccggatgcaa
     1681 catcgacttc gtcgccaccg ggcgcgaagg cgacaccttg caggccgagg ccgtggagcg
     1741 cacgctggcc ggtcgcaccg gcgtctatga cgtcaccgtg cgcgacagcg caggcaacac
     1801 catcgccctg ttccggggca agagctaccg tatctccggc gaggtgatcg ccggactcgc
     1861 cgcagagaac gcctagacgg gtccgcgcgg ccccaagcac acgtagagaa acagaggaga
     1921 ccacaccatg cccgtgaaga gcccccgccc cggcgacctc gacccgatcg agaccgccag
     1981 ccgcgacgaa atttccgccc tgcagctgca gcgcctgcag tggtcggtgc gccacaccta
     2041 cgacaacgtc gcgccctacc gcaagcgctg cgaggaagcg ggcgtgcatc ccgacgacct
     2101 ccagtcgctg gccgacctcg cgaagttccc cttcatgacg aagacggacc tgcgcgacaa
     2161 ctaccccttc ggcctgttcg ccgtgccgcg cgacaaggtc gcccgcctgc acgcctccag
     2221 cggcaccacc ggcaagtcgg tcgtcgtcgg ctacacgaag cgcgacctcg acaactgggc
     2281 cgacgtggtc gcccgctcgc tgcgcgcggc cggcgtgcgt gccggcgaca tggtgcacaa
     2341 cgcctacggc tacggcatgt tcaccggcgg cctcggcgcc cactacggca tcgagcgtgc
     2401 cggctgcacc gcggtgccga tgtccggcgg ccagaccgag aagcaggtgc agcagatcat
     2461 ggacttccgc cccgacgcga tcatggtcac gccgtcctac tcgctggtga tcgccgagga
     2521 gttcgagcgc ctgaacatca agcccgagga catctcgctg aagatcggca tcttcggcgc
     2581 cgagccctgg ggcgaaggca tgcgcagcga gatcgagcgc aagctcggca tcgacgccat
     2641 cgacatctac ggcctcaccg aggtcatggg cccgggcgtg gccagcgagt gcatcgagac
     2701 caaggacggc ccggtgatct gggaagacca cttctacccc gagatcatcg accccgacac
     2761 cggcgaggtg ctgcccgacg gcgaggacgg cgagctggtg ttcacctccc tcaccaagga
     2821 ggccttcccg gtcatccgct accgcacccg cgacctcacc cgcctgctgc cgccgaccgc
     2881 gcgctcgttc cgccgcatcg gcaagatcac cgggcgctcg gacgacatgc tgatcgtgcg
     2941 cggcgtgaac gtgttcccga cccagatcga ggagcagatc atgcgcgacc gccgcctgtc
     3001 ggggaactac cagatcgtgc tcacgcgcga cggccatctc gacaacgtcg agatccgcgt
     3061 cgaggtccag cgcgaactct ccggcaagct cagccgcacc gagaccgaga acatcgcacg
     3121 cgagctgcag caccacatca agaccatcat cggcatctcc accaaggtga acgtgatgga
     3181 gttcgaagcc attccgcgca cgctgaccgg caaggcccgc cgcgtcatcg accagcgccc
     3241 gaagcaactg taaggagctg gccatgactc aagcattcat ctgcgacgcc atccgcaccc
     3301 ccatcggccg ctacggcggc accctggcct ccgtgcgtgc cgacgacctc ggcgccgtgc
     3361 cgctcaaggc cctgatgacc cgcaaccccc aggtcgactg gaccgcggtc gaagacatca
     3421 tctacggctg cgccaaccag gccggcgagg acaaccgcaa cgtcgcgcgc atgtccggcc
     3481 tgctcgccgg gctgccgatc gaggtgcccg gcaccacggt caaccgcctg tgcggctcgg
     3541 gcatggacgc catcggcctg gccgcgcgct cgatcaagtc gggcgagacc gagctgatga
     3601 tcgccggcgg cgtcgagagc atgtcgcgcg cgcccttcgt gatgggcaag gccg
//
LOCUS       DAOQNN010000330         3654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16979_length_3654_cov_7.137538, whole genome shotgun sequence.
ACCESSION   DAOQNN010000330 DAOQNN010000000
VERSION     DAOQNN010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16979_length_3654_cov_7.137538"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PKV97_13945"
     CDS             <1..911
                     /locus_tag="PKV97_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNB07031.1"
                     /translation="VNDLWRTLPAHELRTDARAEARFPPEPEENLLYFVEKNAPLLEP
                     WQREVVRIVRKIAQYFFPQRQTQVMNEGWATFWHYTLLNHLYDEGLLADSFMLEFLQS
                     HTNVVAQPPYNSRWFGGINPYALGFAMWRDIRRICEDPDEEDRRWFPEIAGSDWLETF
                     DFAMRNFKDESFVAQYLSPKVMRDFRLFAVLDDDHEDKLEVSAIHDDRGFRKLREILS
                     DQYNLGAREPNVQVWNVDLRGDRSLTLRHNPFRRRPLGDGTDEVMRHLARLWGFTVRL
                     ERADDDGKVELLHEIRGETTRPRPPG"
     gene            1050..2591
                     /locus_tag="PKV97_13950"
     CDS             1050..2591
                     /locus_tag="PKV97_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HNB07032.1"
                     /translation="MFSPLRPIYPVAGIREIEDKLIPNARPPLMERAGRAAAQDAVRL
                     IMDRPGPILIACGPGNNGGDGFVMARQLAQAGREVVVAFCSAAERLPAEAAKAHADYL
                     AAGGSIVSDLPAAPANGWALVVDALFGIGLGRPIEDRYASWIHTLNAQPCPRMALDVP
                     SGLDADTGSPLGATFRATHTTTFIALKPGLLTNDGPDHCGEISVQRIEIDAPAWLPAR
                     GHAIASSLFRHLLQPRPRNTHKGLYGDAAILGGNAGMVGAALLAGRAALWLGTGRVYV
                     GLLDPAGPAVDFAHPELMLRRAETLPERLSALAIGPGLGTQGASANVLADALARPIPL
                     LLDADALNLLSADAALRRVLCTRGAATVLTPHPAEAARLLGSDTASVQADRLRAALEL
                     ARRYRALVVLKGCGSIVATPDERWFINGSGHSGMASAGMGDVLSGLVTGLLAQGWPPE
                     SALIAGVHLHGAAADRLAREGIGPVGLSASETIDAARGVFNGWLIEAQREPRNTGPHS
                     PRTGR"
     gene            2724..3569
                     /gene="nadC"
                     /locus_tag="PKV97_13955"
     CDS             2724..3569
                     /gene="nadC"
                     /locus_tag="PKV97_13955"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNB07033.1"
                     /translation="MLSEQLRVEIQRNIAASLAEDIGTGDLTARLIAPDTEARGRVIT
                     REDAVICGTAWFDAAFAALSPAAVVHWHVKDGDSVKAGQTLCEIAARARVLLTAERTA
                     LNFLQLLSGTATVTRRFVDAVAGTRAKIVDTRKTLPGLRLAQKYAVAIGGGTNHRVGL
                     YDGILVKENHIIAAGGIREVVEQASAIAPSNVFIEVEVETLGQLREALDAGVKMILLD
                     NMTLEQMCEAVAINAGRAELEASGGVNLERVRAIAETGVDRISIGTLTKDVRALDLSL
                     RHVER"
BASE COUNT          547 a         1459 c         1164 g          484 t
ORIGIN      
        1 aggtcaacga cctgtggcgc acactgcccg cccacgaact gcgcaccgat gcccgggccg
       61 aggcgcgctt cccgcccgag cccgaggaga acctgctcta cttcgtcgag aagaacgccc
      121 cgctgctcga gccctggcag cgcgaggtgg tgcgcatcgt gcgcaagatc gcgcagtact
      181 tcttcccgca gcgccagacc caggtcatga acgaaggctg ggcgaccttc tggcactaca
      241 ccctgctcaa ccacctctac gacgagggtc tgctcgccga cagcttcatg ctggaattcc
      301 tgcagtcgca caccaacgtc gtcgcgcagc cgccctacaa cagccgctgg ttcggcggga
      361 tcaaccccta cgcgctgggc ttcgcgatgt ggcgggacat ccgccgcatc tgcgaggacc
      421 ccgacgagga agaccgccgc tggttccccg agatcgccgg cagcgactgg cttgaaacct
      481 tcgatttcgc gatgcgcaac ttcaaggacg agagcttcgt cgcgcaatac ctgtcgccca
      541 aggtcatgcg cgacttccgc ctgttcgcgg tgctcgacga cgaccacgag gacaagctcg
      601 aggtctccgc gatccacgac gacaggggct tccgcaagct gcgcgagatc ctctccgacc
      661 agtacaacct gggcgcgcgc gaacccaacg tgcaggtgtg gaacgtcgac ctgcgcggcg
      721 accgctcgct caccctgcgc cacaacccct tccgccgccg cccgctcggc gacggcaccg
      781 acgaagtcat gcgccacctc gcccgcctgt ggggcttcac cgtgcgcctc gagcgtgcgg
      841 acgacgacgg caaggtcgaa ctcctccacg agatccgcgg cgagaccacc cgcccccgcc
      901 cgcccggctg agcgcctgtc gcccgaagcg acccgaaggc cagcgcagtt ggagcgcggg
      961 tcgcgctccg gccgccaaga ttgcgcccgc gcgcattggg cgcgcgcagc cgaggacgta
     1021 gaatcccgac tcccccaccg gagtccgtca tgttcagccc cctgcgtccg atctaccccg
     1081 tcgccggcat ccgcgagatc gaggacaaac tcatcccgaa cgcccgcccg ccgctgatgg
     1141 agcgcgccgg gcgcgccgcc gcgcaggatg cggtacggct gatcatggat cgccccgggc
     1201 cgatcctgat cgcctgcggc cctggcaaca atggcggcga cggcttcgtg atggcgcgcc
     1261 agctcgccca ggccgggcgc gaggtggtgg tcgcgttctg cagcgcggcc gagcgtctgc
     1321 cggccgaggc ggccaaggcg cacgccgact acctcgccgc cggcggcagc atcgtctccg
     1381 acctccccgc cgcgccggcc aacggctggg ccctggtggt agacgcgctc ttcgggatcg
     1441 gcctcggacg cccgatcgaa gaccgctacg cgagctggat ccacaccctc aacgcccaac
     1501 cctgcccgcg catggcgctc gacgtcccga gcggactcga cgccgacacc ggcagcccgc
     1561 tcggcgccac cttccgcgcc acccacacca ccaccttcat cgccctcaaa cccggcctgc
     1621 tcaccaacga cggccccgac cactgcggcg agatcagcgt gcagcgcatc gagatcgacg
     1681 cccctgcctg gctgccggcg cgcggtcacg cgatcgcatc ctcgctgttc cgccatctgc
     1741 tgcagccgcg cccgcgcaac acccacaagg gtctctacgg cgacgccgcc atcctcggcg
     1801 gcaacgccgg catggtcggc gccgcgctac tcgccggccg cgccgcgctc tggctgggga
     1861 cgggaagggt ctatgtcggc ctgctcgacc ccgccggccc cgcggtcgac ttcgcccacc
     1921 ccgagctcat gctgcgtcgc gccgagaccc tgcccgagcg cctcagcgcg ctcgccatcg
     1981 gccccggcct gggcacccag ggcgcgtccg ccaacgtgct cgccgacgcc ctcgcgcgcc
     2041 cgatcccgct gctcctcgac gccgacgcgc tcaacctcct ctccgccgat gcggcgctgc
     2101 gccgggtgtt gtgcacgcgc ggcgcagcca ccgtgctcac cccccacccc gcagaggctg
     2161 cccgcctgct cggcagcgac accgcaagcg tgcaggccga ccggctgcgt gccgcgctcg
     2221 aactcgcccg ccgctaccgt gccttggtcg tgctcaaggg ctgtggcagc atcgtcgcca
     2281 cacccgacga gcgctggttc atcaacggca gcggccactc cgggatggca agcgcgggca
     2341 tgggagacgt gctgagcggc ctcgtcaccg gcctgctcgc gcagggctgg ccgcccgagt
     2401 ccgcgctcat cgccggcgta cacctgcacg gtgccgcagc cgaccgactc gcacgcgaag
     2461 gcatcggtcc ggtcgggttg agcgcgagcg agaccatcga cgccgcccgt ggcgtcttca
     2521 acggctggct gatcgaggcg cagcgcgagc cgcgcaacac cggcccccat tccccgcgga
     2581 ccgggcgctg agcgcgccgg tgctagaatc gcagcatccc ggcggcgcca ccgggcgcag
     2641 ccacccggcg cctggcgcgt ctgtgccggg ccgccgcctc gcccggcccg ccgcccttcc
     2701 cgcctaacct tgcaggtcct cccatgctct ccgaacagct ccgcgtcgaa atccagcgca
     2761 acatcgcagc ctcgctcgcc gaggacatcg gcaccggcga cctcaccgcg cgcctgatcg
     2821 caccggacac cgaggcgcgc ggccgcgtca tcacccgcga ggacgcggtc atctgcggca
     2881 ccgcctggtt cgacgccgcg ttcgccgcgc tcagcccggc ggcggtggtg cactggcacg
     2941 tcaaggacgg cgacagcgtc aaggcggggc agaccctgtg cgagatcgcc gcgcgcgcgc
     3001 gcgtgctgct gaccgccgag cgcaccgcgc tcaacttcct gcagctgctc tcgggcaccg
     3061 ccaccgtcac ccgccgcttc gtcgatgcgg tggccggcac ccgcgccaag atcgtcgaca
     3121 cccgcaagac cctgccgggc ctgcgcctgg cgcagaagta cgcggtcgcg atcggtggcg
     3181 gcaccaacca ccgcgtcggc ctctacgacg gcatcctggt caaggagaac cacatcatcg
     3241 cggccggcgg catccgcgag gtggtcgagc aggcaagtgc catcgcgccc tcgaacgtgt
     3301 tcatcgaggt cgaggtggag acactggggc aactgcgcga ggcgctcgat gccggcgtga
     3361 agatgatcct gctcgacaac atgacgctcg agcagatgtg cgaggcggtg gctatcaatg
     3421 ccggccgcgc cgaactcgag gcctcgggtg gcgtgaatct cgagcgcgtg cgcgcgatcg
     3481 ccgagaccgg cgtcgaccgc atctcgatcg gcaccctgac caaggacgta cgagcgctcg
     3541 acctgtcgct gcgccacgtc gagcgctgag ggcgcgggtc cgcccatggc cgccagcgcc
     3601 cccctgcccc ctgccgcgcc cgtcgcttcc gacccgcccg ccctgcccgc ctgg
//
LOCUS       DAOQNN010000331         3653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_16984_length_3653_cov_9.031684, whole genome shotgun sequence.
ACCESSION   DAOQNN010000331 DAOQNN010000000
VERSION     DAOQNN010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16984_length_3653_cov_9.031684"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /gene="tilS"
                     /locus_tag="PKV97_13960"
     CDS             <1..879
                     /gene="tilS"
                     /locus_tag="PKV97_13960"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNB07034.1"
                     /translation="GRVGRLRPLLGVRRAQLLEWAGAHGLGWVEDESNAELRFTRNAL
                     RHRLLPVAEGLFPAAVPNLARAAAHFAEADALLGELAAIDAAACGAPVLCRADLLALT
                     PARQANLLRWLLLGSGAPLPGAAPLGEALRQLRACDPAHPLRLPLGAWVCCAYRDRVW
                     LEPAQPPAPQALHWQGEAAMHWGGDEVRLVASIGAGLGRGCLEDAGEVLLGPRREGLR
                     MRLHPARPRRELRKLCQEAAIPAWLRDRLPVLWVDGEPAWVGGIGVAAEFACAPGAAG
                     LVPEWRPGGVTPAPPP"
     gene            complement(903..1496)
                     /locus_tag="PKV97_13965"
     CDS             complement(903..1496)
                     /locus_tag="PKV97_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB07035.1"
                     /translation="MNPACTHIIDDDEAIRDALEWLFKTRGVPCRSWASAEAFLADWR
                     GCIVLDIRMQGMSGLECFDELLARGCQLPVIFITGHGDVPMAVAALKKGAFHFIEKPF
                     NDHDLVDLVEKALATDDERLRAAASRETIEARLATLTQREREVMELILEGKYNKVIAD
                     DLSISMRTVEAHRSRIFEKMEVRSAVELAQLLTTLRT"
     gene            complement(1493..3487)
                     /locus_tag="PKV97_13970"
     CDS             complement(1493..3487)
                     /locus_tag="PKV97_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB07036.1"
                     /translation="MSLSDPLPPPARRRWLQQLPYLSLALFLAAIAALVWLTREYDDE
                     AQRATLISDVLWMEQNLRFNLDRNENHLQEIGPELLATPTLSAQTEARLASLLTSERG
                     LARILWLSPEGEVRGAMPPLAPAQAVHDASADAAAVPVDESSVRLARAIGRPVYGPAH
                     PAPGGTHHFEVHVPVWSDEAQVGMVVGVYALSDVVMRELPWWFSERYHVAVLDSDGRT
                     IAAKSKVAPVSPRQSYSMPFDPPGHGLTLQISAYRSDPRWIPVLLGASIVLLAGIIVW
                     SVMQLRRQLARRQQAEVALRAESVFRKAMEDSMLTGMRARDLEGRLTYVNSAFCRMTG
                     FSADELLGRKPPMPYWDPERLEQTFELHRQIMTSGSASEGTEVRLRRKNGETLDVLVF
                     EAPLIDASGRHAGWMGSVLDITEQKRAREQARQQEERLQQSSRLITMGEMASTLAHEL
                     NQPLAAIASYTTGCINRLQDEAPLDRSELLDVHQRIARQAQRAGEIIRRVHDFVRRSE
                     PKREALDLAAVIRDAIGLIEADARKRGMRIRSELADGLPQVPADAVMIEQIVVNLVRN
                     AMDSMRDTPPAERVVAVHTAREGRFVTVTVADRGAGIPAETAARLFEPFFTTKQEGMG
                     MGLNICRSIAELHRGRLAFESRPGGGTIFTLSLPVDAEFE"
BASE COUNT          489 a         1237 c         1376 g          551 t
ORIGIN      
        1 gggcgggtgg ggcggctgcg cccgctgctc ggggtgcgcc gcgcgcagct gctcgagtgg
       61 gcgggcgcgc acggactcgg ttgggtggag gacgagagca acgccgagct gcgcttcacc
      121 cgcaacgccc tccgccatcg tctgctgccg gtggccgaag gcctcttccc ggcggcggtg
      181 ccgaacctgg cgcgcgctgc cgcgcatttc gccgaggccg acgcgctgct cggcgaactc
      241 gccgcgatcg atgccgcggc ttgcggcgcg cccgtgctat gccgcgcgga cttgctcgcc
      301 ctgacgccgg cgcgccaggc caacctgctg cgctggctgc tgctcgggtc cggtgcgccc
      361 ttgcccggcg cggcgccgct cggcgaagcc ctgcgccagc tgcgcgcgtg cgatcccgcc
      421 caccccctgc gcctgccgct cggcgcctgg gtgtgctgcg cctaccgcga tcgtgtgtgg
      481 ctggagccgg cgcagccgcc cgctccgcag gcgctgcact ggcagggcga ggcggcgatg
      541 cactggggcg gggatgaagt acgcctcgtg gcgtcgatcg gcgcgggact gggcaggggc
      601 tgcctcgaag atgccgggga ggtcctgctc gggccccggc gcgagggtct gcgcatgcga
      661 ctgcaccccg cgcggccgcg gcgcgagctg cgcaagctct gccaggaggc cgcgattccc
      721 gcgtggctgc gcgaccgcct gccggtgctg tgggtggacg gcgagcccgc gtgggtgggc
      781 ggcatcggcg tggcggcgga gttcgcctgc gcgccggggg cagcggggct cgtgccggag
      841 tggcgaccgg gcggcgtcac ccccgcgccg ccaccctgag cgcggcacgg tggcgcccgc
      901 gctcaggtgc gcagcgtggt cagcagctgt gccagctcca ccgccgagcg cacctccatc
      961 ttctcgaaga tgcgcgagcg gtgcgcctcc acggtgcgca tcgagatcga caggtcgtcg
     1021 gcgatcacct tgttgtactt gccttcgagg atcagctcca tgacctcgcg ctcgcgctgg
     1081 gtgagcgtgg ccaggcgcgc ctcgatggtc tcgcggctgg cggcggcgcg caggcgctcg
     1141 tcgtcggtgg cgagcgcctt ctcgacaagg tcgacgagat cgtggtcgtt gaagggcttc
     1201 tcgatgaagt ggaaggcgcc ctttttgagc gccgccaccg ccatcggcac gtcgccgtgg
     1261 ccggtgatga agatcaccgg cagctggcag ccgcgcgcga gcagctcgtc gaagcactcc
     1321 agcccgctca tgccctgcat gcggatgtca agcacgatgc agccgcgcca gtcggcgagg
     1381 aaggcctcgg ccgaggccca gctccggcag ggtacgccgc gcgtcttgaa cagccattcg
     1441 agggcgtcgc ggatggcttc gtcgtcgtcg atgatgtgcg tgcaggcggg attcattcga
     1501 actcggcgtc caccgggagg gaaagggtga agatggtacc gccgcccggc cgcgattcga
     1561 aggccaggcg gccgcggtgc agctcggcga tcgagcggca gatgttgagt cccatgccca
     1621 tgccctcctg cttggtggtg aagaagggct cgaacagccg cgcggcggtt tcggcgggaa
     1681 tgcctgcgcc gcggtcggcg acggtgacgg tgacgaagcg gccctcgcgc gcggtgtgca
     1741 cggcgacgac gcgctcggct ggcggggtgt cgcgcatcga gtccatcgcg ttgcgcacca
     1801 ggttgacgac gatctgctcg atcatcaccg cgtccgccgg cacctgcggc aggccgtcgg
     1861 cgagctcgct gcggatccgc atgccgcgct tgcgcgcgtc ggcctcgatc aggccgatgg
     1921 cgtcgcggat caccgcggcg aggtcgagcg cctcgcgctt gggttcggag cggcgcacga
     1981 aatcgtgcac ccggcggatg atctcgccgg cgcgctgggc ctggcgggcg atgcgctggt
     2041 gcacgtcgag cagctcgctg cggtcgagcg gcgcctcgtc ctgcaggcgg ttgatgcagc
     2101 ccgtggtgta gcttgcgatc gccgcgagcg gctggttgag ctcgtgcgcg agcgtggacg
     2161 ccatctcgcc catggtgatc aggcgcgagc tctgctgcag gcgctcctcc tgctggcgcg
     2221 cctgttcgcg cgcgcgcttc tgttcggtga tgtcgagcac cgaacccatc cagccggcgt
     2281 ggcggccgga agcgtcgatc agcggcgcct cgaagacgag gacgtcgagg gtctcgccat
     2341 tcttgcgtcg caggcgcacc tcggtgcctt cgctggcgct gccgctggtc atgatctggc
     2401 ggtgcagctc gaaggtctgc tccagccttt ccgggtccca gtagggcatc ggcggcttgc
     2461 gaccgagcag ctcgtccgcg ctgaagccgg tcatgcggca aaaggcggag ttgacgtagg
     2521 tgaggcggcc ttcgaggtcg cgcgcacgca tgccggtcag catcgagtcc tccatcgcct
     2581 tgcggaagac cgactcggcg cgcagcgcca cctcggcctg ctgacggcgt gcgagctggc
     2641 ggcgcagttg catcacgctc cacacgatga tgccggccag cagcacgatc gaggcgccga
     2701 gcagcaccgg gatccagcgc gggtcggagc ggtaggcgga gatctgcagc gtgaggccgt
     2761 gtccgggcgg atcgaagggc atcgaatagg actgccgcgg cgacaccggc gcgactttcg
     2821 acttggcggc gatcgtgcgt ccgtcgctgt cgagcacggc gacgtggtag cgctcggaga
     2881 accaccacgg cagctcgcgc atcaccacgt ccgagagcgc atacacgccg accaccatgc
     2941 cgacctgcgc ctcgtccgac cacaccggca cgtgcacctc gaagtggtgg gtgccaccgg
     3001 gcgccggatg cgcggggcca tacaccgggc ggccgatggc gcgtgccagc cgcacgctgg
     3061 attcgtcgac cggcaccgcg gcagcatccg cggaggcgtc gtgcaccgcc tgggcggggg
     3121 ccagcggcgg catcgcaccg cgcacttcgc cttccggcga cagccacagg atgcgcgcga
     3181 ggccgcgctc ggatgtcagc aggctggcga gccgagcctc ggtctgcgcc gacagcgtgg
     3241 gcgtggcgag cagctcgggg ccgatctcct gcaggtggtt ctcgttgcgg tcgaggttga
     3301 agcgcaggtt ctgctccatc cacagcacgt cgctgatcag cgtcgcgcgc tgggcctcgt
     3361 cgtcgtactc gcgggtcagc cataccagcg cggcgatcgc ggcgaggaag agcgccaggc
     3421 tgaggtaggg cagctgctgc agccagcgcc ggcgcgccgg gggcgggaga gggtcggaga
     3481 ggctcatcgg aaaaggctgc gggtcgggat ccggcggctt gcggttgtca cgcatgcgga
     3541 gatccacaat agcagccctc acagggcgaa acgatactga tccccgacac agcgaaactt
     3601 atcgccgtgc atgcgcccgg taaggagctg tccgggcggc gaaaactacc tag
//
LOCUS       DAOQNN010000332         3642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_17056_length_3642_cov_9.941455, whole genome shotgun sequence.
ACCESSION   DAOQNN010000332 DAOQNN010000000
VERSION     DAOQNN010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17056_length_3642_cov_9.941455"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1180
                     /locus_tag="PKV97_13975"
     CDS             263..1180
                     /locus_tag="PKV97_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB07037.1"
                     /translation="MGNSELNYKHLRYFWTVARVGTIAEAGRVLGLAPHSISAQLATF
                     EGTLGVTLFRRSGRRLALTEAGERILGQAEEIFALGDQIVETLRDDSLRRSLPFRIGI
                     PDSMPKSMVHRLIEPALRLERPGRLVCRESPLSELLAELAVHRLDLVIADRPIPPEVS
                     VRGHEHLLGESTLSVFAAPALAGRLQGDFPRLLDGAPFLLPGEDVAHRGALLQWFERM
                     RIRPAIVAEFDDSALMKAFGQAGDGVFAAPTTIADYVCRQYGVRLLGEVPDARARIYA
                     ITTERRLSHPAMQAIRNAASRALTPAPRA"
     gene            complement(1211..1588)
                     /gene="trxA"
                     /locus_tag="PKV97_13980"
     CDS             complement(1211..1588)
                     /gene="trxA"
                     /locus_tag="PKV97_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNB07038.1"
                     /translation="MATLELTADNFNDTVAHHPIVFLDFWAAWCGPCRNFAPTYEAAA
                     EANPDIVFGKVDTEAQQDLAAAFQIRSIPTIAVIREGVMVFRESGALPANALNQVIEG
                     VRGLDMDQVRAEIAQQQADAQQG"
     gene            1711..2820
                     /locus_tag="PKV97_13985"
     CDS             1711..2820
                     /locus_tag="PKV97_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HNB07039.1"
                     /translation="MRSRAAVAWAAKQPLEVTEVDVAPPKAGEVLVRIVATGVCHTDA
                     YTLSGADPEGIFPAILGHEGGGIVEAVGEGVSSVAVGDHVIPLYTPECGKCKFCLSGK
                     TNLCQAIRATQGKGLMPDGSSRFSKDGKPIFHYMGTSTFSEYTVLPEISVAKIRKDAP
                     LDKVCLLGCGVTTGIGAVRNTAKVEAGASVAVFGLGGIGLSAIIGAVMNKAGRIVAVD
                     INPEKFEIARQLGATDCINPLDYDRPIQEVIVDLTDGGVDYSFECIGNVKVMRSALEC
                     CHKGWGESVIIGVAGAGEEISTRPFQLVTGRVWRGSAFGGVRGRSELPGYVDMAQRGE
                     IPLDTFITHRMGLEDINKAFDLMHEGKSIRSVILY"
     gene            2835..>3642
                     /gene="fghA"
                     /locus_tag="PKV97_13990"
     CDS             2835..>3642
                     /gene="fghA"
                     /locus_tag="PKV97_13990"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HNB07040.1"
                     /translation="MSTAPEQIAANRSFGGWHRRFRHRSAALGCDMVFAIYLPPQAAA
                     GPVPVLYWLSGLTCTDENFMQKAGAQRLAAELGIAIVAPDTSPRGTGVPGDPDGAWDF
                     GLGAGFYVNATEEPWSRHYRMYDYVVTELPALVEANFPVSDARAISGHSMGGHGALVC
                     ALKNPGRYRSVSAFAPIANPSACPWGEKAFSRYLGAERARWLEWDACALIAGASERLP
                     LLVDQGEADGFLAEQLRPEALRTACAAAGHPLTLRLQPGYDHSYYFIASFI"
BASE COUNT          546 a         1273 c         1261 g          562 t
ORIGIN      
        1 gtgcatcatc gtgtcgacct cgttgcggtc caggccgccg tcggcgagca cggtgagggc
       61 gagcaggcgg gcggccgccg gggtgctgtt cgtttcgtag tggcgcatgg cgtggtctcc
      121 tttcgtgtgc ggtctcttgc gtcgccgcgc gctcccgggg gaagcgccgc ggccgacgag
      181 tgcagactaa ggcagaagca tccgccgaaa aagtcgacta cgattagttg attgatcgat
      241 tttttcgacg gaaccgaagg ccatgggcaa cagcgagctc aactacaagc acctgcgcta
      301 cttctggacc gtcgcgcgcg tgggcacgat cgccgaggcc gggcgggtgc tcgggctggc
      361 gccgcactcg atcagcgccc agctcgccac cttcgagggc acgctcggcg tcaccctgtt
      421 ccgccgcagc ggccgtcgcc tggcgctgac cgaagcgggt gaacgcatcc tgggccaggc
      481 cgaggagatc ttcgcgctcg gcgaccagat cgtggagacg ctgcgcgacg acagtctgcg
      541 ccgcagcctg cccttccgca tcggcatccc ggactcgatg cccaagtcga tggtgcatcg
      601 cctgatcgag cccgcgctgc gcctggagcg cccgggccgg ctggtgtgcc gcgagtcgcc
      661 gctgtccgag ctgctcgccg agctcgcggt gcaccgcctc gacctggtga tcgccgaccg
      721 cccgatcccg cccgaggtca gcgtgcgcgg ccacgagcac ctgctcggcg agagcacgct
      781 gagcgtgttc gccgcgccgg cgctggccgg gcgcctgcag ggcgactttc cgcgcctgct
      841 cgacggcgcg cccttcctgc tgccgggcga ggacgtcgcc caccgcggcg cactgttgca
      901 gtggttcgag cgcatgcgca tccgcccggc gatcgtcgcc gaattcgacg acagcgcgct
      961 gatgaaggcc ttcggccagg ccggcgacgg cgtgttcgcc gcccccacca cgatcgccga
     1021 ctacgtgtgc cggcagtacg gggtgcgcct gctcggcgag gtgccggatg cgcgcgcgcg
     1081 catctatgcg atcaccaccg agcgccggct ctcgcatccg gccatgcagg cgatccgcaa
     1141 cgcggcctcg cgcgcgctga cgcccgcgcc gcgggcctga gggtcggccg gaatccctcg
     1201 ccggggtcgt tcagccctgc tgcgcgtccg cctgttgctg ggcgatctcg gcgcgcacct
     1261 ggtccatgtc gaggccgcgc acgccctcga tcacctggtt cagcgcgttc gcgggcagcg
     1321 cgccggactc gcggaacacc atgacgccct cgcggatcac cgcgatggtg gggatggagc
     1381 ggatctggaa ggccgcggcg agatcctgct gggcctcggt gtcgaccttg ccgaaaacga
     1441 tgtcggggtt ggcctcggct gccgcctcgt aggtcggcgc gaaattgcgg cagggaccgc
     1501 accaggccgc ccagaagtcg aggaacacga tcgggtggtg cgcgacggtg tcgttgaagt
     1561 tgtcggcagt aagttcgagg gtggccatgt gcggctccat tcaatattag cgaagggcga
     1621 tgatacgtcg cccgaccgca tgggtcacgg agcggtcggg catttcgcct agaataaccg
     1681 tttggaccca atcgaaaagg aaatcccatg atccgttccc gcgctgccgt cgcctgggct
     1741 gccaaacagc cgctcgaagt caccgaagtg gacgtcgccc cgcccaaggc cggcgaagtg
     1801 ctggtacgca tcgtcgccac cggggtctgc cacaccgacg cctacacgct ttcgggcgcg
     1861 gatccggaag gcatcttccc cgccatcctc ggccacgagg gcggcggcat cgtcgaggcg
     1921 gtgggtgaag gcgtgagctc ggtggcggtc ggcgaccacg tgatcccgct gtacacgccc
     1981 gaatgcggca agtgcaagtt ctgcctgtcg ggcaagacca acctctgcca ggccatccgc
     2041 gccacccagg gcaagggcct gatgcccgac ggcagcagcc gcttcagcaa ggacggcaag
     2101 ccgatcttcc actacatggg cacctcgacc ttctcggaat acaccgtgct gcccgagatc
     2161 agcgtggcga agatccgcaa ggacgccccg ctcgacaagg tctgcctgct cggctgcggc
     2221 gtcaccaccg gcatcggcgc ggtgcgcaac accgccaagg tcgaggccgg cgccagcgtg
     2281 gcggtgttcg gcctcggcgg catcggcctg tcggcgatca tcggcgcggt catgaacaag
     2341 gccgggcgca tcgtcgcggt cgacatcaac ccggagaagt tcgagatcgc acgccagctc
     2401 ggcgccaccg actgcatcaa cccgctcgac tacgaccgcc cgatccagga ggtcatcgtc
     2461 gacctcaccg acggcggcgt ggactactcc ttcgagtgca tcggcaacgt caaggtgatg
     2521 cgctcggcgc tggagtgctg ccacaagggc tggggcgagt cggtgatcat cggcgtggcc
     2581 ggcgcgggcg aggagatctc cacccgcccc ttccagctcg tcaccgggcg ggtgtggcgc
     2641 ggctcggcct tcggtggcgt gcgggggcgc agcgagctgc ccggctacgt cgacatggcg
     2701 cagcgcggcg aaatcccgct cgacaccttc atcacccacc gcatggggct ggaggacatc
     2761 aacaaggcct tcgacctgat gcacgaaggc aagagcatcc gctcggtgat cctgtactga
     2821 tggcggagac cgcgatgtcg accgcccctg agcagatcgc cgccaaccgc agcttcggcg
     2881 gctggcaccg ccgcttccgc caccgctcgg cggcgctggg ctgcgacatg gtgttcgcga
     2941 tctacctgcc cccgcaggcc gcggccggcc cggtgccggt gctgtactgg ctgtcggggc
     3001 tcacctgcac cgacgagaac ttcatgcaga aggccggcgc ccagcgcctg gccgccgagc
     3061 tggggatcgc gatcgtggcg cccgacacca gcccgcgtgg caccggggtg ccgggcgacc
     3121 cggacggcgc atgggacttc gggctgggcg cgggcttcta tgtgaacgcg accgaggaac
     3181 cctggagtcg ccactaccgc atgtacgact acgtggtgac ggaactgccg gcgctcgtcg
     3241 aggcgaactt tccggtgagc gacgcgcgcg cgatctcggg gcattcgatg ggcgggcacg
     3301 gcgcgctggt gtgcgcgctg aagaacccgg ggcgctaccg ctcggtgtcg gccttcgcgc
     3361 cgatcgccaa cccgagtgcc tgcccctggg gcgagaaagc gttctcgcgc tacctcggcg
     3421 cggagcgcgc gcgctggctc gaatgggatg cctgcgcgct gatcgccggc gccagcgagc
     3481 gcctgccatt gctggtggac cagggcgaag ccgacggttt tctcgccgaa cagctccgcc
     3541 ccgaggccct gcgcaccgct tgcgccgccg ccggccaccc gctcacgctg cgcctgcagc
     3601 cgggttacga ccacagctac tacttcatcg ccagcttcat cg
//
LOCUS       DAOQNN010000333         3585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_17534_length_3585_cov_5.345326, whole genome shotgun sequence.
ACCESSION   DAOQNN010000333 DAOQNN010000000
VERSION     DAOQNN010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17534_length_3585_cov_5.345326"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKV97_13995"
     CDS             complement(<1..205)
                     /locus_tag="PKV97_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA synthetase"
                     /protein_id="HNB07041.1"
                     /translation="MPDASESFLAGLVRHGGRPALRGEGGEIAYDRLPEAVAACRAIL
                     DSVGARRVALALDNGPQWALWDLA"
     gene            complement(189..857)
                     /locus_tag="PKV97_14000"
     CDS             complement(189..857)
                     /locus_tag="PKV97_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HNB07042.1"
                     /translation="MDLPWLDHPARPAPGAQPAARSLRLLEVDDARRPAVEHFVRERF
                     AAHYQARVRHFMPRLFALEAADGSLHGAVGCRGAADQGLFLERYLDEPVERVITRRAG
                     ERVARDGIVEVGNLAAHGAGTARLLIIALTRILAAEGLRWVCFTGTPALINSFRRLGL
                     DPLTLGPADPRRMGAELPEWGSYYDAGPLVMAGEIRGGDRTLAGAGRELQSGRGGQLR
                     AGCV"
     gene            1057..1314
                     /locus_tag="PKV97_14005"
     CDS             1057..1314
                     /locus_tag="PKV97_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNB07043.1"
                     /translation="MNRILALAAAALVSTSALAGPTCTAPKEKWMKEADFKAKVEAQG
                     YKIKNFKVTKNDCYEIYGFDKDGKKVEIYFNPETGAELERK"
     gene            1378..1935
                     /locus_tag="PKV97_14010"
     CDS             1378..1935
                     /locus_tag="PKV97_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNB07044.1"
                     /translation="MRPPDSELALRVWDPFVRVFHWSLVSCVILNFFVVDDGETLHRF
                     VGYTASGLVVARLVWGFVGSEYARFANFFPTPVRLRAHLAAMRTGRRDFQPGHNPLGA
                     LMMLALMALVLALGLTGFLQTTDAFWGEEWLEEIHEALAATLIALAGIHAAAAIVMGR
                     LERVNLVGAMITGIKRTRGGRVDLR"
     gene            complement(1972..2718)
                     /locus_tag="PKV97_14015"
     CDS             complement(1972..2718)
                     /locus_tag="PKV97_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNB07045.1"
                     /translation="MHASELFTPLPVPALDASRRWLLAQSALLFLGALLLYGTFERTD
                     LDRRLAHALFDAELGLFPLRHSWFLEAVMHKAAKQATYVLVAASLYLCWQGWRGRLAW
                     LPARNALLAAIGMLAIPLATSTAKLLTARYCPWDLIDFGGYAPYLGLFEAAPPGLKAG
                     QCFPAGHASTGFLWIVWAVALKPAGPRAARAALVAGVLAGGVLGTARMLQGAHFLSHT
                     LWTLWLAWAVSVSLAVLVRADLGLAGRRGA"
     gene            complement(2723..>3585)
                     /locus_tag="PKV97_14020"
     CDS             complement(2723..>3585)
                     /locus_tag="PKV97_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNB07046.1"
                     /translation="HDTTPELARRAVINFPDVTSCGTNTEVSVPCMFSLQGRRNYDED
                     AIRGSESLLDVLRHAGLRVVWNDNQSGCKGVCAGVESLRPDPAALPALCDGERCLDEA
                     LLESSRALLRDPQGNLVLVLHQLGNHGPAYFRRYPEAFRRFTPTCDDEDLSKCTREQV
                     VNSYDNALSYTDHVLARGIDLLKELEPRYDAALLYVSDHGESLGENGLYLHGLPYSIA
                     PAEQTRVPMLMWLSSGFAARNRVDAACLRGQAARPASHDNLFHTVLGLLDVRTAVRDD
                     ALDLTAPCRS"
BASE COUNT          566 a         1256 c         1275 g          488 t
ORIGIN      
        1 gcgcgaggtc ccacagcgcc cactgcgggc cgttgtcgag cgcgagcgcg acgcggcgcg
       61 cccccaccga gtcgaggatg gcacggcacg cggcgacggc ctcgggcagg cgatcgtagg
      121 cgatctcccc gccctcgccc cgcagcgcgg gccgcccgcc gtggcggacg aggccggcca
      181 ggaaggactc agacgcatcc ggcacggagc tgccctccac gcccggattg gagctcgcgg
      241 ccggcgcccg cgagggtgcg atccccgccc cggatctcgc ccgccatcac cagcgggccg
      301 gcgtcgtagt agctgcccca ctccggcagc tcggcaccca tgcggcgcgg atcggcaggg
      361 ccgagcgtga gcggatcgag gccgaggcgg cggaagctgt tgatcagcgc cggcgtgccg
      421 gtgaagcaca cccagcgcag gccttcggcg gccaggatgc gggtgagcgc gatgatcagc
      481 aggcgtgcgg tgccggcccc gtgggcggcg aggttgccga cctctacgat cccgtcgcgt
      541 gccacccgct ctcccgcccg ccgcgtgatg acgcgctcga ccggctcgtc gagatagcgt
      601 tcgaggaaga gcccctggtc tgccgcgcca cggcagccga ccgccccgtg caggctgccg
      661 tcggcggctt cgagtgcgaa cagccgcggc atgaagtggc gtacacgcgc ctggtaatgc
      721 gccgcgaagc gttcgcgcac gaagtgctcc acggcggggc ggcgggcgtc atcgacctcg
      781 agcaggcgaa gactgcgcgc cgcgggctgt gcgcccggcg ctgggcgggc tgggtggtcg
      841 agccatggga ggtccatgtg tgtcggggtc cgttgatgag aactgcaccg gatcctgacc
      901 atgctggctt aagtgcagct taaggaggaa ccaaccgcgg gcgtcgcgca ccggatcacc
      961 gcccctgcct gttcagcttc ccttcatgta cggaagctta aattgcggtc gcgttccgga
     1021 tcggggcgcg ctcgatcgac aaacaggaga acacacatga accgcattct cgccctcgcc
     1081 gccgctgcgc tcgtttccac ctcggccctc gccggcccga cctgcaccgc ccccaaggag
     1141 aagtggatga aggaggccga cttcaaggcc aaggtcgaag cccagggcta caagatcaag
     1201 aacttcaagg tcaccaagaa cgactgctac gagatctacg gcttcgacaa ggacggaaag
     1261 aaggtggaga tctacttcaa tcccgagacc ggcgccgaac tcgaacgcaa gtgacctgcc
     1321 atcggggcgg cgcgcggccg cgcgccgccc tcctccaccc gacaaggagc ctctgccatg
     1381 cgcccgcccg attccgaact tgccctgcgg gtctgggacc cgttcgtgcg cgtcttccac
     1441 tggagcctgg tgagctgcgt catcctgaac ttcttcgtcg tcgatgacgg cgagacgctg
     1501 caccggttcg tcggctacac cgcctcgggt ctcgtcgtcg cccgcctggt ctggggcttc
     1561 gtcggcagcg aatacgcgcg cttcgccaac ttcttcccga cccccgtgcg gctgcgcgcc
     1621 cacctcgcgg cgatgcgcac cggcaggcgc gacttccagc ccggccacaa tcccctgggc
     1681 gcgctgatga tgctcgcgct gatggcgctg gtcctcgcac tcggcctgac cggcttcctg
     1741 cagaccacgg acgcgttctg gggcgaggaa tggctggagg agatccacga ggcgctcgcc
     1801 gccaccctga tcgcactcgc cgggatccat gccgcggcgg ccatcgtgat gggccggctg
     1861 gagcgggtga acctggtcgg cgcgatgatc accgggatca agcgcacgcg gggcggccgc
     1921 gtcgacctgc gctgacggag cagcgctgcg gtccgccgca gcgcggacgc gtcatgcgcc
     1981 gcgccgcccc gcgagaccga ggtcggcgcg cacgagcacc gccaggctca cgctcacggc
     2041 ccacgccaac cacagagtcc acagcgtgtg cgacaggaag tgcgcacctt gcagcatgcg
     2101 cgccgtaccg aggacgccgc cggcgagcac gcccgccacg agcgcggcgc gcgccgcacg
     2161 cggcccggcc ggtttcagcg ccaccgccca gacgatccac aggaagccgg tcgacgcgtg
     2221 cccggccggg aagcactgcc ccgccttgag ccccggcggc gcagcctcga acaggcccag
     2281 atagggtgcg tagccgccga agtcgatgag gtcccagggg cagtagcgcg ccgtcagcag
     2341 cttggcggtc gaggtcgcga gcggaatcgc gagcatgccg atcgccgcga gcagtgcatt
     2401 gcgcgcgggc agccacgcca gccggccgcg ccagccctgc cagcacaggt acaggctggc
     2461 cgccaccagc acgtaggtgg cctgcttggc cgccttgtgc atcaccgcct cgaggaacca
     2521 gctgtgccgc aggggaaaga ggccgagctc cgcatcgaac agcgcgtgcg ccaggcggcg
     2581 atccagatcg gttcgctcga atgtcccgta gagcagcagt gcaccgagga agagcagtgc
     2641 ggactgcgcg agcagccagc gccgcgacgc atcgagcgcg ggcaccggca gcggggtgaa
     2701 gagttcgctg gcgtgcatgt gctcagctcc ggcagggggc ggtcaggtcg agtgcgtcgt
     2761 cgcggaccgc ggtgcgcacg tcgagcaggc ccaggacggt atggaagagg ttgtcatggc
     2821 tggcgggccg ggcggcctgc ccgcgcaggc acgccgcatc gacccggttg cgcgcggcga
     2881 agccggacga cagccacatc agcatcggca cgcgggtctg ctccgcgggc gcgatcgagt
     2941 acggcagccc gtgcaggtag aggccgttct cgccgagcga ctcgccatgg tccgagacat
     3001 acagcagcgc ggcgtcgtag cgcggctcca gctccttcag caggtcgatg ccgcgggcga
     3061 gcacgtggtc ggtgtagctc agcgcgttgt catagctgtt gaccacctgc tcgcgggtgc
     3121 acttcgacag gtcctcgtcg tcgcaggtcg gcgtgaagcg gcggaaggct tccggatagc
     3181 ggcggaagta ggccggaccg tggttgccga gctgatgcag gaccagcacg aggttgcctt
     3241 gcggatcacg gagcagcgcc cgactgctct ccagcaaggc ctcgtcgagg cagcgctcgc
     3301 cgtcgcacag cgcgggcaag gcggcggggt cggggcgcag gctctcgacc ccggcgcaca
     3361 cgcccttgca gccggactgg ttgtcgttcc acaccacccg caggccggcg tgacgcagca
     3421 catcgagcaa ggactcgctg ccgcggatgg catcctcgtc gtagttgcgc cgcccctgca
     3481 gcgagaacat gcagggcacc gacacctcgg tgttggtgcc gcagctcgtg acgtccggga
     3541 agttgatgac cgcgcggcgg gcgagctcgg gcgtggtgtc gtggg
//
LOCUS       DAOQNN010000334         3578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_17578_length_3578_cov_7.166619, whole genome shotgun sequence.
ACCESSION   DAOQNN010000334 DAOQNN010000000
VERSION     DAOQNN010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17578_length_3578_cov_7.166619"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKV97_14025"
     CDS             complement(<1..535)
                     /locus_tag="PKV97_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HNB07047.1"
                     /translation="MAVALASTVCAAAGEGAFERALAEQARYLATEALAYEHGEGVPR
                     DQSYAALLYCESARLGDTEGMYALGWMYANGRGVERNDAYAGTLFAMAAAKGDEHAQR
                     MLRYTGDYTGEVPDCLHTPSTQIVQRWPVEALIARLPAPRQAVARMLAELAPKYGVRP
                     EFALAIGLTESALNPDAL"
     gene            complement(665..3469)
                     /gene="prsT"
                     /locus_tag="PKV97_14030"
     CDS             complement(665..3469)
                     /gene="prsT"
                     /locus_tag="PKV97_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNB07048.1"
                     /translation="MSENRTVRHHPRWSRPIRGIAVAAFSALVLVGCGETPEQMLASA
                     KSFLEKQDFDAAGIQLKNALQENANLAEARFLLGRVNLRQGNVAGAVKELERALELGY
                     PRDAVAAELAPALVRAGEFDRLLAEFGDPRVTAPATIAIVQAALGDAHLARREIDKAQ
                     AAYLQALGANADEHGARIGFARTAAIKGDLKAAEEAVREVIARAPQVADAHALLGDLL
                     VARGDLSQGLAALREAVRLQPGTVANHYALVTLLLRQNAIAEADAALDAMKSAVGRHP
                     FTRYLLALRDYRENRLVEARDALMQVLKESPGLLVAELLAGVVHLRLNDHEIARAHLD
                     RVLQAAPGNLVARQALVASHLATGESKRALELVQPALQVPQPSARLLGLAGQVYIANG
                     DFERAEDYFERAAKAAPADVRARTRLGVARMAVGDADAGFTELEEASRMDEGGIQADL
                     ALVVGHLRRGDAKKALAAQAELERKQPDNALVHNLRGGLMLATRNIPAARAAFEKALA
                     KQPTYLSAAVSLARLDLAERRPEMAIGRIKAVVDRDPRSVEALLTFAEMQRLTGAAPI
                     EVLGTLERAEAVSPGAPVVGLATIQHHLSEGQPAEALRVAQKLATAHPNDPRAIEALA
                     RAQFAAGDSQQAISALNRLVGLLPESPLPLVALADMHRALMDGQAAEQALRKALGIAP
                     DSMDVRQRLIALALQRKDPQGALRLAREAQSRQPELPAGYLLEGDVHAVGSQWPDAAE
                     AYRKALERKGGGTAAVRLHSALLRADRRADANRMSEGWLKANPTDLVLRGHLGELALS
                     EKRYADAARIFARMAEMAPQNPIILNNLAWAANEAKDPKALEYAEQALRLAPDNPAII
                     DTVGTIQVASGAVDAGLANLRRAVSIGPDLLPLQLNLVKALAKAGRKDEARSQLDALM
                     PRLQEGTPMHQEARALQASL"
     gene            complement(3466..>3578)
                     /locus_tag="PKV97_14035"
     CDS             complement(3466..>3578)
                     /locus_tag="PKV97_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB07049.1"
                     /translation="ARTNGNIARAAEILGVSRPSLYDLLSRFGLKKENAS"
BASE COUNT          544 a         1250 c         1238 g          546 t
ORIGIN      
        1 agagcgcgtc ggggttcagg gccgactcgg tcagcccgat cgcgagggcg aactccgggc
       61 gcacaccata cttgggggcg agctcggcga gcatgcgcgc gaccgcctga cgcggcgcag
      121 gcaggcgcgc gatgagcgcc tcgacaggcc agcgctgcac gatctgggtc gaaggcgtat
      181 gcaggcagtc gggcacttcg ccggtgtagt cgccggtgta tcgcaacatg cgctgcgcat
      241 gctcgtcgcc cttcgccgcg gccattgcga agagcgtgcc ggcgtaggcg tcgttgcgct
      301 cgacaccgcg gccgttggcg tacatccagc cgagcgcgta catgccctcg gtgtcgccga
      361 ggcgcgcgga ctcgcagtag agcaaggccg catacgactg gtcgcgcggc acgccctcgc
      421 cgtgttcgta ggccagcgcc tcggtggcga gatagcgggc ctgctcggcg agcgcacgct
      481 cgaacgcgcc ctcaccggcc gcggcgcaaa cggtcgaggc cagcgcgacc gccaacccca
      541 gcagccgtgg aaggcgccgg aggcgtggcc gcgcaatcct gccgaccggg accgggcaac
      601 ttgtcttgtc catgactctt tatcgcacac gccacgctgg atcgccaccc ctgggatcgg
      661 gggatcagag cgacgcttgc agcgcacgcg cctcctgatg catcggggtc ccctcctgca
      721 ggcgcggcat cagcgcatcg agctggctgc gcgcctcgtc cttgcgtccg gccttggcga
      781 gcgccttcac gaggttcagc tgcagcggca gcaagtcggg accgatcgac accgcgcgcc
      841 gcaggttggc caggccggca tcgaccgccc cgctggcgac ctggatggtg cccacggtat
      901 cgatgatcgc cgggttgtcc ggggcgaggc gcagagcctg ctcggcatac tccagcgcct
      961 tcgggtcctt cgcttcgttg gcggcccagg ccagattgtt caggatgatc gggttctgcg
     1021 gcgccatctc ggccatgcgc gcaaagatcc tggccgcatc cgcataccgt ttctcgctca
     1081 gggccagttc gccaagatgg ccacgcagca cgagatccgt cggattcgcc ttcagccacc
     1141 cctcggacat gcgattcgca tcggccctgc ggtctgcgcg gagcagggcc gaatgcaggc
     1201 gtaccgcagc cgtgcctcca cccttgcgct cgagcgcctt gcgataagcc tccgcggcat
     1261 cgggccactg gctgcccacc gcatgcacgt cgccctcgag caggtagcct gcaggaagct
     1321 cgggctgccg cgactgggcc tcgcgggcaa gacgcaacgc accctgcggg tccttgcgct
     1381 gcagcgccag ggcgatcagg cgctggcgga cgtccatgga gtcgggggcg atccccaagg
     1441 ccttgcgcaa ggcctgctcc gccgcctgtc cgtccatcag cgcacgatgc atgtccgcca
     1501 gcgcaacgag cggaagcggc gactcgggca atagcccgac cagccgattg agcgcggaaa
     1561 tcgcttgctg gctgtccccc gcggcgaact gcgcgcgcgc gagcgcctcg atcgcgcgcg
     1621 gatcgttcgg atgcgcggtc gcgagcttct gcgccacccg cagtgcttcc gcaggctgcc
     1681 cttcggagag atggtgctgg atggttgcca gaccgacgac cggggccccg ggcgagacgg
     1741 cctcggcacg ctccagcgtt ccgagcacct cgatcggcgc ggcgccggtg agacgctgca
     1801 tctcggcgaa ggtcagcaag gcctcgacac tcctggggtc gcgatcgacc accgccttga
     1861 tgcgcccgat ggccatctcg ggacggcgct cggccagatc gagccgcgcc aggctgaccg
     1921 cggcggagag ataggtgggc tgcttcgcga gtgccttctc gaacgctgca cgcgcagcag
     1981 gaatgttgcg cgtggccagc atcagcccgc cacgaagatt gtgcacgagc gcattgtccg
     2041 gctgcttgcg ctcgagttcg gcctgtgcgg cgagcgcctt tttcgcgtcg cccctgcgca
     2101 gatgcccgac gacgagggct agatcggcct ggatgccgcc ctcatccatc cgggaggctt
     2161 cctcgagttc ggtgaagccg gcatccgcgt cgccgacggc catgcgcgcg accccaaggc
     2221 gcgttcgcgc acgcacgtcc gccggtgctg ccttcgccgc ccgttcgaaa tagtcctcgg
     2281 cacgctcgaa atccccattg gcgatgtaca cctgccctgc caggccgagc aggcgggcac
     2341 tcggctgcgg aacctggagc gcgggctgga cgagctcgag cgcacgcttc gactcgccgg
     2401 tggcgaggtg cgaggccacc aacgcctggc gagcgacgag atttcccggc gcggcctgga
     2461 gaacccggtc gagatgggca cgcgcgatct cgtgatcgtt cagccgcaaa tgcacgacgc
     2521 ctgcgagcag ttcggcgacg agcagcccgg gcgactcctt gagcacctgc atgagcgcgt
     2581 cgcgggcttc cacgaggcga ttctcgcggt aatcccgcag cgctaggagg taacgcgtga
     2641 aggggtgcct gcccaccgcc gacttcatcg cgtcgagggc ggcatcggcc tcggcgatgg
     2701 cgttctggcg aagcagaagg gtgacgagcg cgtaatggtt ggccaccgtc cccggttgca
     2761 gacgcacggc ctcgcgcaag gcggcgagcc cctgcgacag atcgccgcgg gcaacgagca
     2821 ggtcgccgag cagcgcatgc gcatcggcga cctgcggcgc gcgtgcgatg acctcccgta
     2881 cggcctcttc cgctgctttc agatcaccct tgatcgccgc ggtgcgtgcg aatccgatcc
     2941 gcgccccgtg ctcgtcggcg ttcgccccga gcgcctggag ataggccgcc tgcgccttgt
     3001 cgatctcccg ccgcgccaga tgcgcatcgc ccagcgcagc ctggacgatg gcgatcgtcg
     3061 cgggcgcggt cacccgagga tcgccgaact ccgcgagaag ccggtcgaac tcgcccgccc
     3121 gcaccagcgc cggcgcaagt tcggcggcga ccgcgtcgcg cgggtagccg agctcgagcg
     3181 cccgctcgag ctccttgacc gcgccagcga cgttgccctg acgcaggttg acccgcccga
     3241 ggagaaaacg cgcctcggcg agattggcgt tttcctgcag ggcattcttg agctggatgc
     3301 cggccgcgtc gaagtcctgc ttctccagaa aggacttcgc cgaggcgagc atctgctcgg
     3361 gcgtctcgcc acaaccgaca agaacgagcg ccgagaacgc ggcgacggcg atgccccgaa
     3421 tgggccggga ccagcgcggg tggtgacgga cggtgcggtt ttcgctcacg aagcattctc
     3481 cttcttgagt ccgaagcggc tcaatagatc gtacagggag gggcggctga caccgaggat
     3541 ctcggcggcg cgggcgatgt tgccgttggt gcgggcga
//
LOCUS       DAOQNN010000335         3534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_17957_length_3534_cov_10.956884, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000335 DAOQNN010000000
VERSION     DAOQNN010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17957_length_3534_cov_10.956884"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /gene="glpK"
                     /locus_tag="PKV97_14040"
     CDS             complement(<1..1359)
                     /gene="glpK"
                     /locus_tag="PKV97_14040"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNB07050.1"
                     /translation="MAYLLALDQGTTSSRAIVFDADGRVRGLAQQAFAQHFPAPDRVE
                     HDPQDILRTQLDCAREALANAGVAAREVAAIGIANQRETTVLWERTSGRALAPAIVWQ
                     DRRTAVECERLRAAGCAEDVRARTGLELDPYFSATKLAWLLDHVPDARVRAERGELAF
                     GTIDSWLLWHLSGGRLHLTDAGNAARTLLFNLHTLDWDPVLLERFRIPPVLLPRVVAS
                     SGVCGETDPALFGAAIPIAGIGGDQQAATFGQACLAPGMAKNTYGTGCFLLMNTGHVP
                     VASAHRLLTTVGWTGVDAAPSYALEGSVFMGGAVVQWLRDGLGLVKRASEVEALAASV
                     PDSGGVVLVPAFTGLGAPYWDPHARGTLLGLSRGSGAAHIARAALEAIAMQSADLVQA
                     MARDGAAPLAELRVDGGAAANDLLMQFQADLLGVPVLRPRLLETTAFGAACLAALGCG
                     LWR"
     gene            1483..2355
                     /locus_tag="PKV97_14045"
     CDS             1483..2355
                     /locus_tag="PKV97_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB07051.1"
                     /translation="MPAFWMIVSCFLFACMGVCVKLASGAFSTGEIVFWRGLVSMLMM
                     VGLAASRGIGLKTPHWKMHLSRSVSGSIALGCYFFAIGLLPLATAVTLNYTSPLFVAL
                     VLALFFGERLRAIAVLAILAGFAGVVLLLRPTLQPEQWLGALAGLGSGALASLAYVSL
                     RELGRAGEPEVRTVFWFSVMTSTMGLAWALAGELHRPDAGQLATLLGVGLFGGLAQLA
                     MTRSYRYGRTVVSANLSYATVIFASVFGMLLWDELMPASSWLAVGLIVAGAIGVSLAT
                     PKPAPLPARAVATD"
     gene            2415..2792
                     /locus_tag="PKV97_14050"
     CDS             2415..2792
                     /locus_tag="PKV97_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNB07052.1"
                     /translation="MISTESTDKLVSLVVFGEFTLADYKEFEDVVNYRVQFSGPVDLL
                     FDLRQMAGFTLDVAWEEIKFSRQHAGDFRRIAVLTEDQWLTWSAWVSQLFVTAEVKVF
                     DDEDEARAWLAAETTAETTGSAQ"
     gene            2789..>3534
                     /locus_tag="PKV97_14055"
     CDS             2789..>3534
                     /locus_tag="PKV97_14055"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNB07053.1"
                     /translation="MNASYATLIGAIDLAQHLRDADWVVFDCRADLVDPVFGQRAYDV
                     AHVPGAFFIDLETGLSGPKTGRNGRHPLPDPASLAARLAACGVGNHSQVVAYDDAGGM
                     FAARLWWMLRWLGHHRVAVLDGGLQAWMAAGLALDDEPPRARPATYTLALRPDRVDTQ
                     YVQQHLGRPDMLLLDARSPDRFRGENEVLDPVGGHIPGARNRFFRDNLDARGCFKQAS
                     VLREEFASLLGAYTPRQVVHQCGSGVTACV"
BASE COUNT          488 a         1278 c         1234 g          534 t
ORIGIN      
        1 cctccacagc ccgcagccga gcgcggccag gcaggcggcg ccgaaggcgg tggtctcgag
       61 caggcgtgga cgcagcaccg gcacgccgag caggtcggcc tggaactgca tcagcaggtc
      121 gttggccgcg gcgccgccat cgacgcgcag ctcggcgagc ggcgcggcgc cgtcgcgtgc
      181 catcgcctgg acgaggtcgg cgctctgcat ggcgatcgcc tccagtgccg cacgcgcgat
      241 gtgcgccgca ccgctgccac gcgacaggcc gagcagggtg ccgcgcgcgt gcgggtccca
      301 gtacggcgcg cccagcccgg tgaaggccgg caccagcacc accccaccgc tgtcgggcac
      361 gctcgccgcc aaggcctcga cctcggacgc gcgcttcacc agcccgaggc cgtcacgcaa
      421 ccactgcacc accgcaccgc ccatgaacac gctgccctcg agcgcgtagc tgggcgccgc
      481 atccaccccg gtccacccca cggtggtcaa tagccggtgc gcggacgcca ccggcacatg
      541 gccggtgttc atgagcagga agcagccggt gccgtaggtg ttcttcgcca tgcccggggc
      601 gaggcaggcc tggccgaagg tcgccgcctg ctggtcgccg ccgatgccgg cgatcgggat
      661 cgcagcaccg aacagcgccg gatcggtctc gccgcacacc ccgctgctcg ccaccacgcg
      721 cggcagcagc acgggcggga tgcggaagcg ctcgagcaac accggatccc agtccagcgt
      781 gtggaggttg aagagcagcg tgcgcgcggc attgcccgca tcggtcaggt gcagccggcc
      841 gccgctcaga tgccacagca gccagctgtc gatggtgccg aaggcgagct cgccgcgctc
      901 ggcgcgcacg cgcgcatcgg ggacgtggtc gagcagccag gcgagcttgg tggcggagaa
      961 atagggatcg agctccagcc cggtgcgcgc gcgcacgtcc tctgcgcagc ccgcggcgcg
     1021 caggcgctcg cactcgaccg cggtgcggcg gtcctgccag acgatcgcgg gggcgagggc
     1081 gcgaccgctg gtgcgctccc acagcacggt ggtctcgcgc tggttggcga tgccgatcgc
     1141 cgccacctcg cgtgcggcca cgccggcatt ggcgagcgcc tcgcgggcgc agtcgagctg
     1201 ggtgcgcagg atgtcctggg gatcgtgctc gacccggtcg ggcgcaggga agtgctgcgc
     1261 gaatgcctgc tgggcgaggc cgcgcacgcg gccatcggcg tcgaacacga tcgcgcgcga
     1321 gctggtggtg ccctggtcga gggcgagcag gtaggccatg gcgactccgc acgggggctg
     1381 accgcgatac cgcgagccta gcgccggacg cgccccgccg ccacccggga aagccacgat
     1441 gtgattcaat ggagtcccct tcgttgcagc gcaacaaccg ccatgcccgc cttctggatg
     1501 atcgtctcct gcttcctgtt cgcctgcatg ggcgtgtgcg tgaagctcgc ctcgggggcg
     1561 ttctcgaccg gcgagatcgt gttctggcgc gggctcgtga gcatgctgat gatggtcgga
     1621 ctggcggcga gccgcggtat cggcctgaag acgccgcact ggaagatgca cctgtcgcgc
     1681 agcgtgtcgg gctcgatcgc gctcggctgc tacttcttcg cgatcggcct gctgccgctg
     1741 gcgaccgcgg tcacgctcaa ctacacctcg ccgctcttcg tcgccctggt gctggcgctg
     1801 ttcttcggcg agcgcctgcg cgccatcgcc gtgctcgcga tcctcgccgg cttcgccggc
     1861 gtggtattgc tgctgcgccc cacgctgcag cccgagcaat ggctgggcgc gctcgccgga
     1921 ctgggctcgg gtgccctggc cagcctcgcc tacgtgagcc tgcgcgagct cggccgtgcc
     1981 ggcgagccgg aagttcgcac ggtgttctgg ttctcggtga tgacctcgac gatgggcctg
     2041 gcgtgggcac tcgccggcga gttgcaccgc cccgatgccg ggcaactcgc cacgctgctc
     2101 ggcgtcggcc tcttcggtgg gctcgcccag ctcgcgatga cgcgctccta ccgctacggg
     2161 cgcaccgtgg tgtcggctaa cctgagctac gccaccgtga tcttcgccag cgtgttcggc
     2221 atgctgctgt gggacgaact catgccggcc tcgtcctggc tcgcggtcgg cctgatcgtg
     2281 gccggcgcga tcggcgtctc gctcgccacc ccgaagccgg caccgctgcc ggcccgtgcc
     2341 gtcgcgacgg attgagcccc gccggtgcgc ggactatagt gggggcatct ccgatcacct
     2401 ccagggaggc tgccatgatc agcaccgaga gcaccgacaa actcgtctcg ctcgtcgtct
     2461 tcggcgaatt caccctcgcc gactacaagg agttcgaaga cgtggtcaat taccgggtgc
     2521 agttcagcgg accggtcgac ctgctcttcg acctgcgcca gatggcgggc ttcacgctcg
     2581 acgtggcgtg ggaggagatc aagttctcgc gccagcacgc gggcgatttc cgccgcatcg
     2641 cggtactcac ggaagaccag tggctgacct ggagcgcatg ggtgtcgcag ctcttcgtca
     2701 ccgccgaggt caaggtcttc gacgacgagg acgaggcacg cgcctggctc gccgccgaaa
     2761 cgaccgcaga gaccacggga agcgcgcagt gaacgcttcc tacgcgacgc tgatcggtgc
     2821 gatcgatctc gcgcagcacc tgcgcgacgc cgactgggtc gtgttcgact gccgtgccga
     2881 cctcgtcgat ccggtcttcg gccagcgcgc ctacgacgtc gcgcacgtgc cgggggcctt
     2941 tttcatcgac ctggagaccg ggttgtcggg gccgaagacc ggccgcaacg gccgtcaccc
     3001 cctgcccgat cccgccagcc tcgccgcccg gctcgccgcc tgcggcgtgg gcaatcatag
     3061 ccaggtggtc gcctacgacg acgccggcgg catgttcgcc gcgcgcctgt ggtggatgct
     3121 gcgctggctc ggccatcatc gcgtcgcggt actcgatggc ggcctgcagg cctggatggc
     3181 ggccgggctc gcgctcgacg acgagccgcc gcgtgcgcgc cccgcgacct atacgctggc
     3241 gctgcgtccg gaccgggtcg atacccagta cgtgcagcaa catctcggcc gccccgacat
     3301 gctgctgctc gatgcgcgca gccccgaccg cttccgcggc gagaacgagg tgctcgatcc
     3361 ggtgggcggg cacatccccg gcgcacgcaa ccgcttcttc cgcgacaacc tcgacgcgcg
     3421 cggctgcttc aagcaggcct cggtactgcg cgaagagttc gccagcctgc tcggcgcata
     3481 cacgccgcgc caggtggtgc accagtgcgg ctcgggcgtc accgcctgcg tgaa
//
LOCUS       DAOQNN010000336         3533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_17969_length_3533_cov_8.124209, whole genome shotgun sequence.
ACCESSION   DAOQNN010000336 DAOQNN010000000
VERSION     DAOQNN010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17969_length_3533_cov_8.124209"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PKV97_14060"
     CDS             <1..426
                     /locus_tag="PKV97_14060"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB07054.1"
                     /translation="RMSLAFEQRIVGKQYVAVVHGHLARDDGEIDLPLAEDADHRPRQ
                     VVDPLRGRRALTRYRVLDRQGHDAEASSRLELKPVTGRAHQLRVHLRSLGHPILGDAL
                     YAPPESAARFARMHLHATRIAFMHPHTRVPMLFDSPAPF"
     gene            488..1582
                     /locus_tag="PKV97_14065"
     CDS             488..1582
                     /locus_tag="PKV97_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HNB07055.1"
                     /translation="MTIRSPLQRVADLLRTEWHHLTAFTPSTRRWQLAFAAALASGLP
                     LMVGAWFGHLEYGLISSIGGLALLYLPNTPMSHRMVWLMACAFGMAASYALGLMTHFL
                     PLAKVGVLALIATLVTMVCRFYRVGPPGSLFFVMAAAIGAYTPGTVEEIPLRVGLLTM
                     GALLASLIAFVYSLQVLRLQPADAVPPLQPPTFDFVVFDSVVIGAFVGLSLAIAQALQ
                     LDRPYWVPVSCLAVIQGVSLRAVWNRQLQRVLGTAIGMLFAWWLLSLPLDAWRIALLV
                     ILLVFVIESLVVRHYGLAVIFITPLTILLAEAATLGHAPLAELIASRFIDTLLGCVVG
                     LVGGICLHSARFRTLFGNPMRRLIPRQPPS"
     gene            1617..2573
                     /locus_tag="PKV97_14070"
     CDS             1617..2573
                     /locus_tag="PKV97_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB07056.1"
                     /translation="MKINQLRYFLAVADKGSVRAAATALGVSAAAVSQALRELETAMG
                     TPLFTRESHGAVLSYAGRQFLIHARLILGQVSRAEAEIAQIRGATGGSLTIGVTPWVA
                     QSILPHALARFHALRPDVHLDVTEAVGTVHPLLRDGTLDLVIAIPPPRQEASIFYTRE
                     LFRCDLAVVGRLGHPFASATSLEELVDQDWVLTMLGAGQDEPLVNLLAPHGIAPPPHR
                     VHYARSALVAISMLEVGNMLTICPWPLLETPLLRGRVQALPIRNPLPEMHTAIIVRRN
                     DTLSAAGQLFIDCFEDATRTCVATDDPALKRIMNSVERAPDA"
     gene            complement(2697..2966)
                     /locus_tag="PKV97_14075"
     CDS             complement(2697..2966)
                     /locus_tag="PKV97_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07057.1"
                     /translation="MQAPKILPWVARKAGISEGDALDAWRRALVDAAAYVGVRSGATF
                     DRVAVDRFVALAHARATVLSARAARPAPSVTRRWPPGAVAQRCAA"
     gene            3209..>3533
                     /locus_tag="PKV97_14080"
     CDS             3209..>3533
                     /locus_tag="PKV97_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNB07058.1"
                     /translation="MSSTLTDLLSTALAARAPLIAQLAAEDTDAWRLFHGTVEGAPGI
                     TVDRYGSVLLAQTFHRPLSAEQLAELEAFYAAALPGLALVWNDRSGKNSRVANPLPPE
                     KQALAE"
BASE COUNT          503 a         1336 c         1130 g          564 t
ORIGIN      
        1 cgcatgagcc tcgccttcga gcagcgcatc gtcggcaagc agtacgtggc cgtggtgcac
       61 gggcatctcg cgcgggacga cggcgagatc gacctgccgc tcgcggagga cgcggaccac
      121 cgcccccgcc aggtcgtcga tccactgcgc ggccggcgcg cgctcacccg ctaccgcgtg
      181 ctcgatcgcc agggacacga cgccgaggcc agcagccggc tggaactcaa gccggtcacc
      241 ggacgcgcgc atcagctgcg cgtacacctg cgctccctcg gccaccccat cctcggcgat
      301 gcgctgtacg ctccgcccga gtccgccgcc cgcttcgccc gcatgcacct gcacgccacc
      361 cgcatcgcct tcatgcatcc gcacacccgc gtgccgatgc tgttcgacag cccggcgccc
      421 ttctgaagcc gctccccctt gccacccgcg gcgtgctata catcgtgccc tcgaccggta
      481 gaagcgcatg acgatccgct ccccgctgca acgcgtcgca gacctgctgc gcaccgagtg
      541 gcaccacctc accgcgttca cccccagcac ccggcgctgg caactggcct tcgccgccgc
      601 gctggcctcc ggcctgccgc tgatggtggg ggcatggttc ggccatctcg aatacggact
      661 gatctcgtcg atcggcgggc tcgcgctcct ctacctcccc aacacgccga tgtcgcaccg
      721 catggtgtgg ctgatggcct gcgccttcgg catggcggcg agctatgcgc tcgggctgat
      781 gacgcatttc ctgccgctgg ccaaggtagg cgtgctggcg ttgatcgcga cgctggtgac
      841 catggtgtgc cgcttctatc gcgtcggccc cccgggcagc ctgttcttcg tcatggccgc
      901 ggcgatcggc gcctacacgc ccgggacggt ggaggagatc ccgctgcgcg tgggcttgct
      961 gacgatgggc gcgctgctcg cgagcctgat cgccttcgtc tacagcctcc aggtcctgcg
     1021 cctgcagccg gccgatgcgg tgccgccgct gcagccgccg accttcgact tcgtcgtctt
     1081 cgattcggtg gtgatcggcg cctttgtcgg cctgtcgctg gcgatcgccc aggcgctcca
     1141 gctcgaccgc ccctactggg tgccggtgag ctgcctggcg gtgatccagg gcgtctcgct
     1201 gcgcgcggtg tggaaccgcc agctccagcg cgtgctcggc accgccatcg gcatgctctt
     1261 cgcctggtgg ctgctgagcc tgccgctcga cgcgtggcgg atcgcgctgc tggtgatcct
     1321 gctcgtcttc gtgatcgaga gcctggtggt gcgccactac gggctcgcgg tgatcttcat
     1381 cactccgctc acgatcctgc tcgccgaggc cgccaccctg ggccacgcgc cgctcgccga
     1441 gctgatcgcg tcgcgcttca tcgacaccct gctcggctgc gtcgtcggtc tggtgggcgg
     1501 catctgcctg cacagcgcgc gcttccgcac gctcttcggc aacccgatgc gccggctgat
     1561 cccacgccag ccgccatcct gaccggcttc agcccgtagc cggaccgcca gcaccgatga
     1621 aaatcaacca gttgcgctat ttcctcgccg tggccgacaa gggcagcgtg cgcgcggccg
     1681 caacggcact cggggtctcg gctgccgccg tcagccaggc cctgcgcgaa ctcgagacgg
     1741 cgatggggac gccgctgttc acgcgcgagt cgcacggcgc ggtgctgagc tacgccggac
     1801 gacagttcct catccacgcc cgcctgatcc ttggccaggt cagccgcgcc gaagccgaga
     1861 tcgcacagat ccgcggcgca accggtggct cgctgacgat cggggtcacg ccctgggtcg
     1921 cgcagtccat ccttccccac gccctggccc gcttccacgc cctgcgcccg gatgtgcatc
     1981 tggacgtcac cgaggcggtg ggcacggtcc atccgctgct gcgcgacggc accctcgacc
     2041 tggtgatcgc gattccgcca ccacgccagg aggcgtcgat cttctacacg cgcgagctgt
     2101 tccgctgcga tctggctgtc gtcggccgcc tcggccatcc ctttgccagc gccacttcgc
     2161 tcgaggagct cgtcgaccag gactgggtgc tgacgatgct cggcgccggc caggacgagc
     2221 cgctggtcaa cctgctcgcc ccccacggca tcgcgccgcc gccgcaccgt gtgcattacg
     2281 cgcgctcggc gctggtcgcc atcagcatgc tcgaggtcgg caacatgctc accatctgcc
     2341 cctggcccct gctcgagacc ccgctgctgc gcgggcgcgt gcaggccctg ccgatccgca
     2401 acccgctgcc cgaaatgcac accgccatca tcgtccgccg caacgatacg ctgagcgcgg
     2461 cggggcagtt gttcatcgac tgcttcgagg acgccacccg gacctgcgtg gccaccgacg
     2521 atcccgcgct caagcgcatc atgaactcgg tcgagcgggc gcccgacgca tgatccgccg
     2581 ggaagaaacc aaaaaaatgc ccgcaagctg cgggcatgaa ccacattgaa gtggaggagg
     2641 gagaccagaa aggatttgca ggcggtcgcg tggcaccacg ccacgccctt gcaggctcag
     2701 gcggcgcagc gttgcgcgac cgcgcccggc ggccaccgcc gggtcacgct gggcgccggg
     2761 cgcgcggcgc gtgccgacag gacggtagcg cgggcatggg cgagggcgac gaagcgatcg
     2821 accgcaacgc gatcgaaggt cgcgccgctg cgcacgccga cgtacgcggc cgcatcgacc
     2881 aaggcgcgtc gccaagcatc gagggcatca ccttcactga tgccggcctt gcgggcaacc
     2941 cagggcagaa ttttgggggc ttgcatggtg agaactcctg tgaatttcgt cgaaggccgc
     3001 gcgccggtga gcctggttgc acgcctcttg cagtgcacaa atcttatggg acgatggacc
     3061 gtccgaatag acaggcattt ccgtgggcgt aaggtttttg tttacgcggg acgacgcccg
     3121 ctggtcgcac tacccgcggg gcaggacagg cgccagcccg ggccggcgcg cttcgggcat
     3181 actggcgcgc cccgcgcagg aacccaccat gtcctccacc ctcaccgacc tgctctcgac
     3241 cgcgctcgcc gcccgcgcac cgctgatcgc acagctggct gccgaagaca ccgacgcctg
     3301 gcgcctcttt cacggcaccg tcgagggcgc gcccggcatc acggtcgacc gctacggcag
     3361 cgtgctgctg gcgcagacct tccatcgccc gctcagcgcc gagcagctgg ccgagctcga
     3421 ggccttctac gctgccgccc tgcccggcct ggcgctggtg tggaacgacc gcagcggcaa
     3481 gaactcgcgc gtcgccaatc cccttccgcc cgaaaaacag gccctcgccg agc
//
LOCUS       DAOQNN010000337         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_17976_length_3532_cov_7.029336, whole genome shotgun sequence.
ACCESSION   DAOQNN010000337 DAOQNN010000000
VERSION     DAOQNN010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17976_length_3532_cov_7.029336"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="PKV97_14085"
     CDS             complement(<1..1478)
                     /locus_tag="PKV97_14085"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNB07059.1"
                     /translation="MAQPGPSDTRSEPREQPPRRRIRPVHVAGAYTAVGLAWIFISDT
                     IAHGLTRSSTAAFNADVIKGSLFVVLSGVLVFVLASRLVRSADASEKRAEASRRQFEE
                     SKRLLEAFSAHCPASMYLVGDDGRIRMVNPRTAQHLGRAPEALVGVGMSELFDPAHAA
                     AVERQHAQILASGSPEMLEEHVRLPDGQLRHFSSFKFPVFEPGGRAVAIGGFSIDVTD
                     TRRAEARYRLLFEHTSSGITINDKGGRYLDVNPEGCRLLGASRDEILRRSAGELDLRQ
                     RIDAVGPGSEAAASEAERSGVMRFRNFAGEERAVDFVAHPLPDGTYLTVFSDATARLR
                     EERVRAEYTRALEMLAGDAPLSRILEEIVRTAEQVDPRVLGSVVLLGDDGRTMRRVVA
                     PSMPRAYSDALLGVEIGPDRGSCGTSMFTGLRVVAHDIETDPRWADFRTLTREHGLRA
                     CWSEPIRLASGEVVGSFAMYYREPRSPSESETAVITGWAHLVG"
     gene            complement(1523..2047)
                     /locus_tag="PKV97_14090"
     CDS             complement(1523..2047)
                     /locus_tag="PKV97_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNB07060.1"
                     /translation="MKLIADEARVDQLISDLGRQIIAGTVERERLALIGIRRRGDVLA
                     ARIGQKLGVKDVGSIDITLYRDDLSETGAAARVGKTNIPFDIEGREIVLCDDVIMTGR
                     SVLAAIREILDFGRPRRLRLCVLVERPERELPLQPEHSALMLHPGPGEKVDVRLKGQD
                     EVEGVWISQKSEKA"
     gene            complement(2097..2381)
                     /locus_tag="PKV97_14095"
     CDS             complement(2097..2381)
                     /locus_tag="PKV97_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07061.1"
                     /translation="MATLEPDRPTPRTLPRWLTKPLLALIVSALASGLLLVLLLVIGQ
                     LMAPGLVGVKAAVRVALASWFLGFWVILWRVVFPARREEPAQERPPTGAA"
     gene            complement(2422..3528)
                     /locus_tag="PKV97_14100"
     CDS             complement(2422..3528)
                     /locus_tag="PKV97_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB07062.1"
                     /translation="MTDTHPHNPTPAPTNSPSLVGDPAAAIAQVRQAYERLHTEVHKV
                     IVGQEAVVDQVLMSIFCRGHALLEGVPGLAKTLLISTLARTLSLGFARIQFTPDLMPS
                     DITGTEVIEEDKSTGVRELRFVKGPIFANVILADEINRTPPKTQAALLEAMQEHQVTV
                     GGVQHRLPQPFFVLATQNPIEQEGTYPLPEAQLDRFMFNIRISYPTAEEELEIVRRTT
                     STGRGTPQAVLSAQDIARVQELVRAVEVPDYVARYAIRIVRATRVSEPMEAGLERPKL
                     VKSYLGWGAGPRASEYLILAAKAKAVLGGSMHVTPEHIQAIAKPVLRHRLLTNFNAEA
                     DQVTTDIIIESLLRDIPVEGLGGPQKRQMDAVMN"
BASE COUNT          501 a         1248 c         1252 g          531 t
ORIGIN      
        1 ccgaccaggt gcgcccagcc ggtgatgacc gccgtctccg actcgctggg cgagcggggc
       61 tcgcggtagt acatggcgaa cgagcccacc acctcgccgc tcgccagccg gatcggctcg
      121 gaccagcacg cgcgcagccc gtgctcgcgc gtgagcgtgc ggaaatcggc ccagcgcggg
      181 tcggtctcga tgtcgtgcgc gaccacccgc aggccggtga acatcgacgt gccgcacgag
      241 ccccggtcgg gcccgatctc cacccccagc agcgcgtcgg aataggcgcg cggcatgctg
      301 ggggccacga cgcggcgcat cgtgcgcccg tcgtcgccca gcagcaccac cgaccccagc
      361 acgcgcggat ccacctgctc ggcggtgcgc acgatctcct cgaggatgcg gctcaggggg
      421 gcgtcgccgg cgagcatctc cagcgcgcgg gtgtactcgg cgcgcacacg ctcctcgcgc
      481 aggcgggcgg tcgcgtcgct gaagacggtc aggtaggtgc cgtcgggcag cgggtgggcg
      541 acgaagtcca cggcgcgttc ctcgccggcg aagttgcgga agcgcatgac gcccgagcgt
      601 tcggcctccg acgccgccgc ctcagaaccg ggcccgaccg cgtcaatccg ctgccggagg
      661 tccagttcgc cggcgctgcg ccgcaggatc tcgtcacgcg atgcgccgag caggcggcac
      721 ccctcggggt tcacgtcgag gtagcgcccc cctttgtcgt tgatcgtgat gccgctggac
      781 gtgtgctcga agagcaggcg gtagcgggcc tcggcgcggc gtgtgtcggt cacgtcgatc
      841 gaaaagccgc cgatggccac ggcgcgcccg ccgggctcga agaccgggaa cttgaacgac
      901 gaaaagtggc gcagctgccc gtcggggagc cgcacgtgct cctcgagcat ctccgggctg
      961 ccggacgcga ggatctgtgc gtgctggcgc tcgacggcgg cggcgtgcgc ggggtcgaac
     1021 agttccgaca tgccgacgcc cacgagcgcc tcgggcgcgc gccccaggtg ctgcgccgtc
     1081 cgcgggttga ccatgcggat gcgcccgtca tcgccgacga ggtacatcga cgccgggcag
     1141 tgggccgaga acgcctcgag caggcgcttg ctctcctcga actggcggcg cgaggcctcg
     1201 gcgcgctttt ctgaggcgtc ggcgctgcgc accaggcgcg atgccagcac gaagaccagc
     1261 acgccgctga gaaccacaaa gagcgagccc ttgatcacgt cggcgttgaa cgccgccgtc
     1321 gagctgcgcg tcaggccgtg cgcgatcgtg tcgctgatga agatccaggc cagcccgacg
     1381 gcggtgtagg cccccgcgac gtgcacgggg cggatgcgcc gtcggggcgg ctgctcacgg
     1441 ggttcggacc gggtgtcgga cggaccgggc tgggccatgc cgcatcatcg ccgaccggat
     1501 cgttgcgcac aagcgcgggc ggtcaggcct tttcgctctt ctggctgatc cagacgccct
     1561 cgacctcgtc ctgacccttc agacgcacgt ccaccttctc ccccgggccg gggtgcagca
     1621 tgagcgccga gtgctcgggc tgcagcggca gttctcgctc cggacgctcg accagcacgc
     1681 acaggcgcag ccggcgcggg cgcccgaagt cgaggatctc gcggatcgcc gccagcaccg
     1741 agcgcccggt catgatcacg tcgtcgcaca acacgatctc gcgcccctcg atgtcgaagg
     1801 ggatgttggt cttgccgacg cgcgccgccg cgcccgtctc cgacaggtcg tcgcggtaga
     1861 gcgtgatgtc gatcgagccg acgtccttga cgccgagttt ctgcccgatg cgtgcggcca
     1921 ggacgtcgcc gcggcgccgg atcccgatga gcgccaggcg ctcccgttcc acggtgccgg
     1981 cgatgatctg gcgtccgagg tccgagatga gttggtccac gcgcgcttcg tccgcgatga
     2041 gcttcatgcc gccagtataa cgccggcgga tgctgcgccc cgcgttcgcg gtcgattcag
     2101 gcggcgccgg tcggcggccg ctcctgcgcc ggttcttcgc ggcgcgccgg gaacaccacc
     2161 cgccacagga tgacccagaa ccccaggaac cacgaggcga gcgcgacgcg aaccgccgcc
     2221 ttgacgccca ccaggcccgg cgccatcaac tggccgatca ccagcagcag cacgagcagc
     2281 aggcccgacg ccagcgccga gacgatcagc gcgagcagcg gtttcgtcag ccagcgcggc
     2341 agggtgcggg gcgtagggcg gtcgggctcc agagtcgcca tcgctggtcg gctcccggga
     2401 agagcgtgcg ggggtcggcg gtcagttcat cacggcgtcc atctggcgct tctgcggtcc
     2461 gcccaggccc tcgacgggga tgtcgcgcag gagcgactcg atgatgatgt cggtggtcac
     2521 ctggtccgcc tcggcgttga agttcgtcag caggcggtgg cgcagcaccg gcttggcgat
     2581 cgcctggatg tgctcgggcg tgacgtgcat cgacccgccc agcaccgcct tggccttggc
     2641 cgcgaggatg aggtactcgc tcgcgcgcgg ccccgcgccc cagcccaggt aactcttgac
     2701 gagtttcggg cgctcgagcc cggcctccat cggctcgctc acgcgtgtgg cgcggacaat
     2761 gcggatcgcg taccgcgcca cgtagtccgg cacctccacc gcgcggacca gttcctgcac
     2821 acgggcgatg tcctgagcgc tcagcaccgc ctgcggcgtg ccgcgaccgg tgctggtcgt
     2881 gcgacgcacg atctccagtt cctcctccgc cgtcgggtaa ctgatccgga tgttgaacat
     2941 gaaccggtcc aactgcgcct cgggcagcgg gtaggtgcct tcctgctcga tcgggttctg
     3001 cgtcgccagc acgaagaacg gctgcggcag ccggtgctgc acgccgccga cggtcacctg
     3061 gtgctcctgc atcgcttcga ggagcgcggc ctgggtcttg ggcggggtgc ggttgatctc
     3121 gtcggccagg atcacgttcg cgaagatcgg gcccttgacg aaccgcagct cgcgcacgcc
     3181 cgtgctcttg tcctcctcga tcacttccgt gccggtgatg tcgctgggca tcagatcggg
     3241 cgtgaactgg atgcgcgcaa accccaggct cagcgtccgc gccagcgtcg agatcaagag
     3301 cgtcttggcc agccccggca ccccctccaa cagcgcgtgg ccgcggcaga agatcgacat
     3361 cagcacctgg tcgaccaccg cttcctgtcc cacgatcacc ttgtgcacct cggtgtgcag
     3421 acgctcgtac gcctggcgca cctgcgcgat cgcggcggcg gggtcgccga cgagcgaggg
     3481 ggagttggtc ggtgcggggg tcgggttgtg ggggtgggta tcggtcatgg gg
//
LOCUS       DAOQNN010000338         3518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18089_length_3518_cov_12.984118, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000338 DAOQNN010000000
VERSION     DAOQNN010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18089_length_3518_cov_12.984118"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PKV97_14105"
     CDS             complement(<1..1272)
                     /locus_tag="PKV97_14105"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765267.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HNB07063.1"
                     /translation="MLEAYRAHVAERAALGIPPLPLSKQQATELVELLKNPPAGEEAF
                     LVELLTYRVPAGVDDAAKVKAEFLARVAKGEEACALVSRAKATELLGTMLGGFNIKPM
                     IDLLADAEVGAVAAEGLKKTLLMFDYFHDVKELAEKGNANAKAVMQSWADAEWFTSRP
                     EVPASQKLTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRPGITPEEAGVRG
                     PVKFLEELRAKGNLVAYVGDVVGTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGS
                     KIAPIFFNTMEDAGALPIEIDAGQMDMGDEIELKVDHASGKVTALKNGAVISESQLKT
                     LVILDEVRAGGRIPLIIGRGLTTKAREALGLPPSTLFRLPQAPADTGKGFSLAQKMVG
                     RACGMPEGQGIRPGTYCEPKMTTVGSQDTTGP"
     gene            1679..2785
                     /locus_tag="PKV97_14110"
                     /pseudo
     CDS             1679..2785
                     /locus_tag="PKV97_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
     gene            complement(2818..>3518)
                     /locus_tag="PKV97_14115"
     CDS             complement(2818..>3518)
                     /locus_tag="PKV97_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HNB07064.1"
                     /translation="FTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAFEALKPMI
                     GGTLNIKNVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIER
                     TLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLN
                     LVTAAIVLWNTVYLERAANALRSHGQVIDDSLLQCLSPLGWEHINLTGDYLWRSSAKI
                     GAGKFRPLRPLQPA"
BASE COUNT          553 a         1162 c         1208 g          595 t
ORIGIN      
        1 cgggccggtg gtgtcctggc tgccgacggt ggtcatcttg ggttcgcagt aggtacccgg
       61 gcggatgccc tggccttccg gcatgccgca ggcgcggccg accatcttct gcgccaggct
      121 gaagcccttg ccggtatcgg cgggggcctg cggcaggcgg aacagcgtcg agggcggcag
      181 gccgagggcc tcgcgcgcct tggtggtgag gccgcggccg atgatcagcg gaatgcggcc
      241 accggcgcgc acttcgtcga ggatgaccag ggtcttgagc tgcgattcgg agatcacggc
      301 gccgttcttg agcgcggtga ccttgccgga agcgtggtcg accttgagct cgatctcgtc
      361 gcccatgtcc atctggccgg cgtcgatctc gatcggcagc gcgccggcgt cttccatggt
      421 gttgaagaag atcggggcga tcttggagcc gaggcacacg ccgccgaaac gcttgttcgg
      481 gacgaagggg atgtcctcgc cggtgaacca cagcaccgag ttggtggcgg acttgcgcga
      541 ggaaccggtg ccgaccacgt caccgacgta ggcgaccagg ttgcccttgg cacgcagctc
      601 ttcgaggaac ttgaccgggc cgcgcacgcc cgcttcttcc ggggtgatgc ccggacgcgg
      661 gttcttgagc atggccagcg cgtgcagcgg gatgtcgggg cgcgaccagg cgtcgggtgc
      721 gggcgacagg tcgtcggtgt tggtctcgcc ggtgaccttg aagacggtga gcttttgcga
      781 ggcggggact tccgggcggc tggtgaacca ctcggcgtcg gcccacgact gcatcacggc
      841 tttggcgttg gcgttgccct tctcggccag ctccttgacg tcgtggaagt agtcgaacat
      901 cagcagggtc ttcttgagac cctcggcggc gaccgcgccc acttcggcgt cggccagcag
      961 atcgatcatc ggcttgatgt tgaagccgcc cagcatggtg ccgagcagct cggtggcctt
     1021 ggcgcgcgac accagggcac aggcttcctc gcccttggcg accctggcca ggaactcggc
     1081 cttgaccttg gcggcgtcat ccacgccggc ggggacgcgg taggtgagca gctcgacgag
     1141 gaaggcctcc tcgccggcgg gggggttctt gagcaactcg acgagttcgg tggcctgctg
     1201 cttggagagc ggcagcggcg ggatgccgag ggcggcacgt tcggcgacat gggcacggta
     1261 ggcttcaagc acggcgactt cctcactatg tgacggtgat cgggactccg gacgggtcgg
     1321 ccccaccgga acgggcgaga ttatatgtct tttataagac ttgcggtgca gtgcaacacc
     1381 atggctcgtc gcccgacatt cgagccggaa gattctacgc cttcgtcacg ctgcggggga
     1441 tatccccgcg cgccgcatgc gttcgctgac cggcagggtc ttgcggacga tcccgcagag
     1501 ggtgtgccgt agagcgatgc aagtcgcgat tgcgcgtctg caccccgccg tcgcccgtcg
     1561 ccaccacgcc atcacgactc gcgtcgctcc cacaggaacg cacgatttgc gcctgcagga
     1621 acttccccgc cgcgcagcga tcgtttgtat tggtcccaac aagaaaagga gacccacgat
     1681 gtctgccacc ccgttcgata cgctccgccc cctgcccgcg tccgccgccc gcctctccta
     1741 tcactcgctg ccggcgctcg aagaggccgg cgtgggccgc atctcgcggc tgccggtgtc
     1801 gatccgcatc gtgctcgagg ccgtgctgcg ccactgcgac ggccacaagg tgaccgccga
     1861 gcacgtgcgc gaactcgcca actgggaggc cgacgcacca cgcaccgccg agatcccctt
     1921 cgtggtcgcg cgcgtggtgc tgcaggactt caccggcgtg ccgctgctcg ccgacctcgc
     1981 cgcgatgcgc agcgtggccg tggcgcaggg ccgcgaggcc aagctcatcg agccgctcgt
     2041 gccggtcgac ctggtggtcg accactcggt gcagatcgac cactacggca cgccggacgc
     2101 gctgcgccgc aacatggaga tcgagttcga gcgcaaccac gagcgctacc agttcatgaa
     2161 gtggggcatg caggccttcg acagcttccg cgtggtgccg ccgggcatcg gcatcgtgca
     2221 ccaggtcaac ctcgaacacc ttttccgcgg cgtgcgcacg cgcgatggcc tgtgcttccc
     2281 cgacaccctg gtgggcaccg actcgcacac caccatgatc aacggcatcg gcgtggtggg
     2341 ctggggcgtg ggcggcatcg aggccgaagc ggcgatgctc ggccagccgg tgtacttcct
     2401 cacccccgac gtgatcggcg tggaactgcg cggcagcctg cccgccggca ccaccgcgac
     2461 cgacctggtg ctgacggtga ccgagctgct gcgccgcgag aaggtggtgg gcaagttcgt
     2521 cgagttcttc ggcgagggca ccgccagcct gtcggtcacc gatcgcgcca ccctcgccaa
     2581 catggcgccg gagtatggcg ccaccatggg cttcttcccg gtggacgcga agaccatcga
     2641 ctacatgcgc gccaccggcc gcgaagaggc cgaatgcgcg ctgatggaag gctacttccg
     2701 cgcgcaaggc ctgttcggcg tgccacgcgc gggcgagatc gactacacgc gcagcctgat
     2761 gctggacctc gccacgatcg agccggggtc gcctcagaaa acggaaaaaa tcgtacgcta
     2821 agccggttgc agcggtcgta gcggcctgaa cttgcccgcg ccgatcttgg cgctgctgcg
     2881 ccagaggtaa tcgccggtca ggttgatgtg ctcccagccc agcggcgaca ggcactgcaa
     2941 caggctgtca tcgatgactt ggccgtggct acgcagggcg ttcgccgccc gctccagata
     3001 gaccgtgttc cacaacacga tggccgccgt caccaggttg aggccgctgg cccggtagcg
     3061 ctgctgctcg aagctgcggt cgcggatttc gcccagccgg ttgaagaaca cggcgcgggc
     3121 cagcgcgttg cgggcttcgc ccttgttcag cccggcatgc acgcggcggc gcagctcgac
     3181 gctttgcagc cagtccagaa tgaacagcgt gcgctcgatg cgccccagct cgcgcagggc
     3241 cacggccagg ccgttctggc gcgggtagct gccgagcttc ctgagcatca gcgaagccgt
     3301 cacagtaccc tgctttatcg aagtggccag ccgcaggatt tcgtcccaat gggcacggac
     3361 gttcttgatg ttgagcgtgc cgccgatcat gggcttcagt gcctcaaagg cagcatcgcc
     3421 cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgtggcgcga agcgaaagcc
     3481 cagcaggtgc atcagcgcga agacgtgatc ggtgaagc
//
LOCUS       DAOQNN010000339         3517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18098_length_3517_cov_14.697574, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000339 DAOQNN010000000
VERSION     DAOQNN010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18098_length_3517_cov_14.697574"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /gene="sdhA"
                     /locus_tag="PKV97_14120"
     CDS             complement(<1..1354)
                     /gene="sdhA"
                     /locus_tag="PKV97_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HNB07065.1"
                     /translation="MNVAKRTFDAVIVGAGGAGLRAALQLSEAGLKTAVLTKVFPTRS
                     HTVAAQGGVAASLGNSTEDNWHWHMYDTVKGSDWLGDQDAIEFMCKKANEVVIELEHY
                     GMPFDRTDNGKIYQRPFGGHSMNYGQAPVMRSCAAADRTGHAMLHALYQRNVRANTQF
                     FVEWMALDLIRDADGDVLGVTAMEMETGEVSIFHAKATIFATGGAGRIYYSSTNAFIN
                     TGDGVGMAARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILRNAQGERFMERY
                     APNLKDLASRDVVSRAMTTEINEGRGCGPDKDHVLLDITHLAPDTIMKRLPGIREIAI
                     QFAGVDPIKAPIPVVPTCHYQMGGIPTNYKGQVVVPKDGNPNSPVAGFYAAGECACAS
                     VHGANRLGTNSLLDLLVFGKSAGETVVEDFQSGQLKLKPLPADAADVSLARLARLDGQ
                     T"
     gene            complement(1357..1707)
                     /gene="sdhD"
                     /locus_tag="PKV97_14125"
     CDS             complement(1357..1707)
                     /gene="sdhD"
                     /locus_tag="PKV97_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNB07066.1"
                     /translation="MVKQRIVVGAHYGLKDWIAQRATAVYMAAYTIIFALCAFSLREL
                     NYQEWSGLFSHGLFKFLSFLFFLSVFYHAWIGIRDLWMDYIKPNGVRLALHLVTIFVL
                     VGYAGWAAQILWRL"
     gene            complement(1701..2090)
                     /gene="sdhC"
                     /locus_tag="PKV97_14130"
     CDS             complement(1701..2090)
                     /gene="sdhC"
                     /locus_tag="PKV97_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNB07067.1"
                     /translation="MSEVTARKPRPKYLALNEIRLPLPGIVSILHRISGVGIFLMLPF
                     LLYLLDRSLGSPETFETFAAVVDNWFIKLVLFGLLWAYLHHFCAGIRFLLLDLHKGLE
                     LEEARKSSKIVLIVSLTLTLIIGVVLW"
     gene            complement(2190..2918)
                     /locus_tag="PKV97_14135"
     CDS             complement(2190..2918)
                     /locus_tag="PKV97_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNB07068.1"
                     /translation="MSQESPTFSPLYRQIKALILQALEAGEWRPGQAIPSEQELAARF
                     NVSQGTVRKAIDEMAADNLLVRKQGKGTFVASHNDPRALFRFLRLVPVDGDLSPPQSI
                     PLDCWRAKAGQEASRMLAIEPGAPIIIVRRLLKFAARPVVIDEIYLPGEVFQGLSAET
                     LQGWNGSLYSLFETRFGVRMIRAQERIRAVAADRGAAEALGVQEGTPLLSVERVTYTY
                     GDRPVEWRRGLYSTAEHFYLNELN"
     gene            3162..>3517
                     /locus_tag="PKV97_14140"
     CDS             3162..>3517
                     /locus_tag="PKV97_14140"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435434.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNB07069.1"
                     /translation="MSKAPMRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLDL
                     PQAQKAVKGVMMELEDCAFPLLAGMIATDDPNVAFKDADVCLLVGARPRGPGMERADL
                     LTANGAIFTVQGKAIA"
BASE COUNT          647 a         1104 c         1181 g          585 t
ORIGIN      
        1 tggtctggcc gtcgaggcgg gccaggcggg cgagcgagac gtcggcagcg tcggccggca
       61 acggcttgag cttgagctgg ccggactgga agtcctcgac cacggtctcg cccgccgact
      121 tgccgaacac cagcaggtcg agtagcgagt tggtgccgag gcggttggcg ccgtgcaccg
      181 aggcgcaggc gcactcgccc gcggcgtaga agccggcgac cgggctgttc gggttgccgt
      241 ccttcggcac cacgacctgg cccttgtagt tggtcgggat gccgcccatc tggtagtggc
      301 aggtcggcac gaccgggatc ggcgccttga tcgggtcgac gccggcgaac tggatcgcga
      361 tctcgcggat gccgggcagg cgcttcatga tggtgtcggg cgcaaggtgc gtgatgtcga
      421 gcagcacgtg atccttgtcg ggaccgcagc cacgaccttc gttgatctcg gtggtcatcg
      481 cgcgcgaaac cacgtcgcgc gaggcgagat ccttgaggtt gggcgcgtag cgctccatga
      541 agcgctcgcc ctgggcgttg cgcaggatgc cgccctcgcc gcgcacgcct tcggtgatga
      601 gcacgcccgc gccggcaacg ccggtggggt ggaactgcca gaactccatg tcctcgagcg
      661 ggatgccggc acgcgccgcc atgccgacgc cgtcgccggt gttgatgaag gcgttggtcg
      721 aggaataata gatgcggccg gcaccgccgg tagcgaagat ggtggccttg gcgtggaaga
      781 tcgagacctc gccagtctcc atttccatcg cggtgacgcc gagcacgtcg ccatcggcgt
      841 cgcggatcag gtcgagcgcc atccattcga cgaagaactg ggtgttggcg cgcacgttgc
      901 gctggtagag cgcgtgcagc atggcgtggc cggtacggtc ggccgcggcg caggagcgca
      961 tcaccggcgc ctggccgtag ttcatcgagt ggccgccgaa cggacgctgg tagatcttgc
     1021 cgttgtcggt gcggtcgaag ggcatgccgt agtgctcgag ctcgatgacg acctcgttgg
     1081 ccttcttgca catgaactcg atggcatcct ggtcgcccag ccagtccgac cccttgacgg
     1141 tgtcgtacat gtgccagtgc cagttgtcct cggtcgagtt gcccagcgag gcggcgacgc
     1201 cgccctgcgc cgccacggtg tgcgagcggg tcgggaacac cttggtcagc acggcggtct
     1261 tgaggccggc ttcggagagt tgcagggccg cgcgcaggcc ggcaccgccg gcgcccacga
     1321 tgaccgcatc aaaggtacgc ttcgcgacgt tcacgcttaa agcctccaca ggatctgggc
     1381 tgcccagccg gcatagccga cgagcacgaa aatggtgacc aggtgcagcg cgaggcgcac
     1441 accgttcggc ttgatgtagt ccatccagag gtcgcggatg ccgatccagg cgtgatagaa
     1501 gaccgacagg aagaacagga agctgaggaa cttgaacaag ccgtgcgaga acaggccgga
     1561 ccattcctgg tagttgagct cgcgcaggct gaacgcacag agcgcgaaga tgatggtgta
     1621 ggccgccatg tagacggcgg tggcgcgttg cgcgatccag tccttcagac cgtagtgcgc
     1681 gccgacgacg atacgctgtt tcaccatagg accaccccga tgatcaaggt gagcgtaagg
     1741 ctcacgatca gaacgatttt ggaggacttg cgtgcttcct cgagctccag acccttgtgc
     1801 aggtcgagca gcaggaagcg gatgccggca cagaagtggt gcaggtaggc ccacagcagg
     1861 ccgaacagga cgagcttgat gaaccagttg tcgaccaccg cggcgaaggt ctcgaaggtc
     1921 tccggagagc cgaggctgcg atcgagcagg tacaacagga aaggcaacat caggaagatg
     1981 ccgacgccgc tgatgcggtg aaggatcgag acgatgcccg gcaacggcag ccggatctcg
     2041 ttgagggcca ggtacttcgg ccttggtttg cgcgctgtta cttctgacat gaagactccc
     2101 ctctgcatgt gcgacgaccc ccgtcgcgga ctccgaaaaa agacgaaaaa ttatagcgcc
     2161 ggtcccggcc tgcagcccat gcgcacccct caattcagct cattcagata aaaatgttcc
     2221 gccgtcgaat acagcccgcg acgccactcc acgggccgat cgccataagt gtacgtgaca
     2281 cgctcgaccg acagcagcgg cgtgccttcc tggacgccga gcgcctcggc ggcgccacgg
     2341 tcggcggcga ccgcgcggat gcgctcctgg gcgcggatca tgcgcacgcc gaagcgggtc
     2401 tcgaagagac tgtagagcga accgttccag ccctgcaggg tctcggcgct gagtccctgg
     2461 aacacctcgc cgggcagata gatctcgtcg atgacgacgg gtcgggcggc aaacttcaac
     2521 aagcggcgga cgatgatgat cggggcgccg ggctcgatcg cgagcatgcg cgaagcctcc
     2581 tgcccggcct tcgcacgcca gcaatccagc ggtatgcttt gcggaggcga gagatcacca
     2641 tccaccggca cgaggcggag gaaacggaag agcgcgcgcg gatcgttgtg agaggccacg
     2701 aaggtgccct tcccctgctt gcgcacgagc aggttgtcgg ctgccatctc gtcgatagcc
     2761 ttgcgcacgg taccctgcga cacgttgaag cgcgcggcga gttcctgctc gctcggaatg
     2821 gcctggccgg gacgccattc cccagcctca agtgcctgaa ggatcagggc cttgatctgg
     2881 cgatagagcg gactgaaggt aggcgactcc tgcgacatgc gcgcattgga gcataaattt
     2941 tcgcaaacgt ctatctttgg tcttgtgtct tatataagat ataagatggc atttgacagg
     3001 cattcaagcc cggtctaata tgccggcgcc cagcttggtc ctcgcgcacg cgggggggcg
     3061 ccgcgccagc tcccgcgacg atcccgcctg cgggtgccgc gacggtgccg ggcaggtttc
     3121 cagcacctta atacgaccgt ctcactcgag ggagtaatca catgagcaaa gcccccatgc
     3181 gcgtcgctgt caccggcgcc gccggccaga tcggctacag cctgctgttc cgcatcgcca
     3241 gcggcgaaat gctgggcaag gaccagcccg tcatcctgca actgctcgac ctgccccagg
     3301 cccagaaggc cgtcaagggc gtgatgatgg agctggaaga ctgcgccttc ccgctgctcg
     3361 ccggcatgat cgccaccgac gatcccaacg tcgcattcaa ggacgccgac gtgtgcctgc
     3421 tggtcggcgc ccgtccgcgc ggccccggca tggagcgtgc cgacctgctg accgccaacg
     3481 gcgccatctt caccgtccag ggcaaggcca tcgccga
//
LOCUS       DAOQNN010000340         3516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18105_length_3516_cov_11.064432, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000340 DAOQNN010000000
VERSION     DAOQNN010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18105_length_3516_cov_11.064432"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2181)
                     /locus_tag="PKV97_14145"
     CDS             complement(<1..2181)
                     /locus_tag="PKV97_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNB07070.1"
                     /translation="MTWILLFSLPPLAGALAYGRAPLFAWLGAGLAWLLAMGGAAGWG
                     FWLTLFAMAVFAGVLSVFLVDSLRMKWLTSRIFGAFRATLPTMSQTEKDALEAGTVWW
                     EGELFAGKPDWKKLQAYPWPTLSAEEQAFLDNETDELCRLTHDWECTRHGDMPAEVWR
                     YIKAKGFLGMIIPKEYGGKGFSAYAHSQVVTKLSTRSSAPAVTVMVPNSLGPAELLLH
                     YGTPEQKQHYLPRLATGQEIPAFALTSPWAGSDAASIPDAGVVCKGMWNGEEVLGMRV
                     NFDKRYITLAPVCTVFGLAFHLHDPERLLGGDEVLGITCALIPHDHPGVDIGRRHFPL
                     NAVWMNGPVRGKDVFVPLDFIIGGPKMAGQGWRMLMECLAAGRSISLPGSNTGMQKMV
                     ARAVGAYARVRYQFKTAIGRFEGVEEALTRIGANTYLSDAARVMTAGAIDLGEKPSVV
                     SAIVKYHVTERARQTVNDGMDVIGGKGICLGPQNFLGRAYQQIPVGITVEGANILTRS
                     LILFGQGAIRCHPYVLAEMHSAQKGDMKAFDKALWGHVGFTVSNAARALIMGLTGSHF
                     VKVPGDVAPETRRYYQQLTRFSAAFAFIADLSMGTMGGALKRKEKLSARLGDILSLMY
                     LASATLKRFESEGRHGADAPLMHWAIWDCMFRIQQAFEGVIANFPNKLFAFVLRRVVV
                     FPLGRPYVVPSDRLGHKVAALLLEPSPTRDRLTSDVHLPTDIEEP"
     gene            complement(2236..2919)
                     /locus_tag="PKV97_14150"
     CDS             complement(2236..2919)
                     /locus_tag="PKV97_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNB07071.1"
                     /translation="MLISMTSTPPAPTNDTRDRILDAAERLFVENGFDGTSMRMITGA
                     ANANLAAVNYHFGGKDALVQEVFRRRLAALNARRLDELDRLEAEAAGAPLKPSRIVEA
                     FFGTALELAADTEHGGRTFMRLLARTYNEPNAFVRQFLAEEYAEVMDRFLGALFRALP
                     EVPRAEILWRFHFMIGAMAFAIAGLGGLSELMEVGPQDEDPARLLPRLMSFLLGGLRA
                     PLPDGGLQG"
     gene            3054..>3516
                     /locus_tag="PKV97_14155"
     CDS             3054..>3516
                     /locus_tag="PKV97_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNB07072.1"
                     /translation="MRRAPSAALPAPATGERHDWQTLKSLLPFIWAYKGRVLFALACL
                     VAAKGANVSVPIIFKHLIDGLTITPEQAFIVVPAALLLAYGALRFSTSLLTELREVVF
                     ARVTQQAVREISLRVFRHLHALSLRFHLERQTGGLTRDIERGTRSIGSLISY"
BASE COUNT          590 a         1162 c         1207 g          557 t
ORIGIN      
        1 cggctcctcg atgtcggtgg gcagatgcac gtcggaggtc aggcggtcgc gcgtcggcga
       61 gggctcgagc agcagcgcgg cgaccttgtg accgagcctg tccgagggca cgacgtaggg
      121 gcggccgagc gggaacacca cgacgcggcg cagcacgaag gcgaagagtt tgttggggaa
      181 gttcgcgatc accccctcga aggcctgctg gatgcggaac atgcagtccc agatcgccca
      241 gtgcatcagc ggcgcgtcgg cgccatggcg gccctcggac tcgaagcgct tgagcgtggc
      301 gctggcgagg tacatcagcg acaggatgtc gccgaggcgg gcggaaagct tctccttgcg
      361 cttgagcgcg ccgcccatgg tgcccatcga gaggtcggcg atgaaggcga aggcggccga
      421 gaagcgggtg agctgctggt aatagcgccg cgtctcgggc gccacgtcgc cgggcacctt
      481 gacgaagtgc gaaccggtga ggcccatgat cagcgcgcgc gcggcgttcg acacggtaaa
      541 gccgacgtgg ccccacagcg ccttgtcgaa ggccttcatg tcgcccttct gcgcggaatg
      601 catctcggcg agcacgtagg gatggcagcg gatcgcgccc tgaccgaaca ggatcaggct
      661 gcgggtgagg atgttggcgc cttccacggt gatgccgacc gggatctgct ggtaggcgcg
      721 gccgaggaag ttctgcgggc cgaggcagat gcccttgccg ccgatcacgt ccatgccgtc
      781 gttaaccgtc tggcgggcgc gctcggtgac gtggtacttg acgatcgccg acaccaccga
      841 gggcttctcg ccgaggtcga tcgcgccggc ggtcatcacg cgcgccgcgt cgctcagata
      901 ggtgttggcg ccgatgcggg tgagcgcctc ttccacgccc tcgaagcggc cgatcgcggt
      961 cttgaactgg tagcgcacgc gggcataggc gccgacggcg cgcgccacca tcttctgcat
     1021 gccggtgttg gagccgggca acgagatgct gcgcccggcc gccaggcact ccatcagcat
     1081 gcgccagccc tggcccgcca tcttcggccc gccgatgatg aaatcgagcg gcacgaacac
     1141 gtccttgcca cgcaccgggc cgttcatcca caccgcgttg agggggaagt ggcgacgacc
     1201 gatgtcgacg ccgggatggt cgtgcgggat gagcgcgcag gtgatgccga gcacttcgtc
     1261 accaccgagc aggcgctcag ggtcgtgaag atggaaggcc agcccgaaca cggtgcacac
     1321 cggggcgagc gtgatgtagc gcttgtcgaa attgacccgc atgccgagca cttcctcgcc
     1381 gttccacatg cccttgcaca ccacgccggc gtcggggatg gaggcggcgt ccgagcccgc
     1441 ccatggactg gtgagcgcga aagccggaat ctcctggccg gtagccaggc gcggaaggta
     1501 gtgctgcttc tgctcgggcg tgccgtagtg cagcagcaac tcggccgggc cgagcgagtt
     1561 cggcaccatg acggtgaccg ccggcgccga cgagcgcgta gacagcttgg tcaccacctg
     1621 ggagtgcgca taggccgaga agcccttgcc gccatattcc ttgggaatga tcatgccgag
     1681 gaagcccttg gccttgatgt accgccagac ttcggcgggc atgtcgccgt gccgggtgca
     1741 ttcccagtca tgggtcaggc ggcagagctc gtcggtctcg ttgtcgagga aggcttgctc
     1801 ctcggcgctc agcgtcggcc agggataggc ctgcagcttc ttccagtccg gcttgccggc
     1861 gaagagctcg ccttcccacc acaccgtacc ggcttccagc gcgtccttct cggtctgcga
     1921 catggtgggc agggtggcgc ggaaggcccc gaagatgcgc gaagtcagcc acttcatgcg
     1981 cagcgaatcg accaggaaga cgctcagcac gccggcgaac accgccatcg cgaacagcgt
     2041 cagccagaag ccccagccgg cggcgccccc catcgccagc agccaggcca accccgcccc
     2101 gagccaggca aaaagtggcg ctcgcccgta ggcgagcgca cctgccaagg gagggaggga
     2161 aaacagcaga atccaggtca tcatcttcat ctcctcttgc cggcgcgccg gagggcgtac
     2221 cgctcgaaat cgtcgtcagc cttgcagacc accgtcgggc aggggcgcac gcaggccgcc
     2281 gagcaggaaa ctcatcaggc gcggcagcag ccgggcggga tcctcgtctt gcggccccac
     2341 ttccatcaat tcgctcaatc cgccgagtcc ggcgatcgca aaggccatcg caccgatcat
     2401 gaagtggaag cgccacagga tctctgcgcg cggcacctcg ggcagggcgc ggaagagcgc
     2461 gccaaggaag cgatccatca cctcggcgta ctcctctgcg aggaactggc gcacgaaggc
     2521 gttgggctca ttgtaggtac gcgcgagcag gcgcatgaag gtgcgcccgc cgtgctcggt
     2581 gtcggcggcg agttccagcg cggtgccgaa gaaggcctcg acgatgcggc tgggcttgag
     2641 cggggctccc gcggcctcgg cctccaggcg gtcgagttcg tcgaggcggc gcgcgttgag
     2701 ggcggcgaga cggcggcgga aaacctcctg cacgagcgcg tccttgccgc cgaagtggta
     2761 attgaccgcg gcgaggttcg cattcgccgc gccggtgatc atgcgcatcg aggtgccgtc
     2821 gaagccgttc tccacgaaaa ggcgctcggc tgcatcgagg atgcggtcgc gggtgtcgtt
     2881 ggtcggcgcg ggcggggtcg aagtcatcga gatcagcatg tttcaaacgt ttgtttgaat
     2941 catacgtttt ctttatcgcc tgtcaagccg ggctgatcct ggagcctgcc gtttgactcg
     3001 cagcagggct gcgcaacaga atcgggcgcc ttccacgcac cggcagacct ccgatgagac
     3061 gcgccccctc cgccgccctg cccgcccccg ccaccggcga acgccacgac tggcagacgc
     3121 tcaagagtct gctgcccttc atctgggcct acaagggccg ggtgctcttc gcgctcgcct
     3181 gcctggtcgc ggccaagggc gccaacgtgt cggtgccgat catcttcaag cacctgatcg
     3241 atggcctcac gatcacgccc gaacaggcct tcatcgtggt gccggcggca ctgctgctgg
     3301 cctacggtgc gctgcgcttc tccacctcgc tgctgaccga actgcgcgag gtggtgttcg
     3361 cacgcgtgac ccagcaggcg gtgcgcgaga tctcgctgcg cgtgttccgc cacctgcacg
     3421 cgctgtcgct gcgtttccac ctcgaacgcc agaccggcgg gctcacgcgc gacatcgagc
     3481 gcggcacgcg ctcgatcggc tcgctgatca gctaca
//
LOCUS       DAOQNN010000341         3516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18106_length_3516_cov_8.297024, whole genome shotgun sequence.
ACCESSION   DAOQNN010000341 DAOQNN010000000
VERSION     DAOQNN010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18106_length_3516_cov_8.297024"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1906
                     /locus_tag="PKV97_14160"
     CDS             <1..1906
                     /locus_tag="PKV97_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB07073.1"
                     /translation="ALQAPAAPIRAEEAGAGAEAAPEVEPLASPTLRVRAERIDRFVN
                     QAGEIGVARSRVEGELRSLRRSLLDLTENVIRLRNQLREVEIQAEVQMQSRIAQAGTQ
                     HTDFDPLELDRYTRLQELTRMMAESVNDVTTVQQGLLHSLDGAELALGSQARMARELQ
                     QGLMQVRMLPFDNLASRLYRVVRQSARELGKRAALDLRGGRIEVDRSVLEQMAAPLEH
                     LLRNAVAHGIEAPAARLAAGKAETGNIELTVRQEGNEIAIEIADDGAGLDLERIAAQA
                     RARGLLGADERVDERRLVNLIFVSGFSTAREVSAVSGRGVGMDVVKAQTAAAGGRVEV
                     ASRPGAGTRFSIRLPLTLAVTQTLLVEALGRSWAVPANMIVQAMELKPDALQAMQRAH
                     GIEWQGEHYPYRYLPRLLGDREARPAEARYSWVLLLRAGAQTLALHVDGLRGNQEVIV
                     KNAGPQLTRVVGMSGATVLGDGEIVLILNPVALGARAATTTDEAHAGLFGPAAEAPPP
                     LHLPTVMVVDDSLTVRKITGRLLEREGYRVLTAKDGVDALEQLIDLKPDVVLTDIEMP
                     RMDGFDLVRNIRADARLHDLPVVVITSRLAEKHRRYALEVGADHYLGKPYQEEELLAL
                     VARYAQAGKG"
     gene            complement(2083..3243)
                     /locus_tag="PKV97_14165"
     CDS             complement(2083..3243)
                     /locus_tag="PKV97_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNB07074.1"
                     /translation="MTAPAFDFDRLIDRRSLPSEKWGRYAGRDVLPLWVADMDFAAPP
                     AVIEALHRRIDHGVFGYTDAWPALEQAVVEGLRRDHGWTIQPDWLVWLPGVVAGFNLA
                     CAIAGEAGDGVLTATPVYPPFLTAPANTGRVLQRAELVLGDGRWQWDWDALEAACTPT
                     TRLLLLCSPHNPVGRVFDEAELRRLADFAARHDLLVCSDEIHCGLVLDADRTHRPLAA
                     LDEAIARRTITLMAPSKTWNIPALYCAFAIIPDPALRRRYRHAMRGIVPHVNVLGMVA
                     AEAAYRDGDAWRRALLDYLRGNRERVLEVLGAMPGLTTTRPEATYLAWVDCRAMMAAR
                     GVSDPAAFFEAAGVGLSDGAAFGAPGFVRLNFGCPRATLDEALARMARALGA"
     gene            complement(3373..>3516)
                     /locus_tag="PKV97_14170"
     CDS             complement(3373..>3516)
                     /locus_tag="PKV97_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HNB07075.1"
                     /translation="WTMGGIDQTACRTKIGVDYPGPIVDHAAARERTLQRFGVVKNDA
                     QGT"
BASE COUNT          515 a         1280 c         1269 g          452 t
ORIGIN      
        1 ggccctccag gcgccggccg ccccgatccg cgccgaggaa gccggcgccg gcgcggaggc
       61 ggcacccgag gtcgagccgc tcgcctcccc gaccctgcgt gtgcgcgccg agcggatcga
      121 ccgcttcgtc aaccaggccg gcgagatcgg cgtcgcgcgc agccgggtcg agggcgagct
      181 gcgcagcctg cggcgctcgc tgctcgatct caccgagaac gtgatccgcc tgcgcaacca
      241 gctgcgcgag gtcgagatcc aggccgaggt gcagatgcaa tcgcgcatcg cgcaggccgg
      301 cacccagcac accgatttcg atccgctcga gctcgaccgc tacacccgcc tgcaggagct
      361 cacgcggatg atggccgaga gcgtcaacga cgtgaccacc gtgcagcagg gcctgctgca
      421 cagcctggac ggcgcggagc tcgccctcgg cagccaggcg cgcatggcgc gcgagctgca
      481 gcaagggctg atgcaggtgc gcatgctgcc cttcgacaac cttgccagcc ggctctaccg
      541 ggtggtgcgg cagagcgcgc gcgagctcgg caagcgtgcc gcgctcgacc tgcgcggcgg
      601 acgcatcgag gtcgaccgca gtgtgctcga gcagatggcc gcgccgctcg aacacctgct
      661 gcgcaacgcg gtcgcgcacg gcatcgaggc cccggccgcg cgcctggccg ccggcaaggc
      721 cgagaccggc aacatcgagc tgacggtgcg ccaggagggg aacgagatcg ccatcgagat
      781 cgccgacgac ggcgccgggc tcgacctcga gcgcatcgcg gcgcaggcgc gcgcgcgcgg
      841 tctgctcggc gccgacgagc gcgtcgacga gcgccgcctg gtgaacctga tcttcgtctc
      901 cggcttcagc accgcgcgcg aggtctccgc ggtctccggc cgcggcgtcg gcatggacgt
      961 ggtgaaggcg caaaccgccg ccgccggtgg ccgcgtcgag gtcgccagcc ggcccggcgc
     1021 cggcacccgc ttctcgatcc gcctgccgct caccctcgcg gtaacccaga ccttgctggt
     1081 ggaggcgctc ggccgcagct gggccgttcc cgccaacatg atcgtgcagg ccatggaact
     1141 caagcccgac gcgctgcagg ccatgcagcg ggcgcacggc atcgaatggc agggcgagca
     1201 ttatccctac cgctatctgc cgcgcctgct cggcgatcgc gaggcgcgcc ccgccgaggc
     1261 gcgctacagc tgggtgctgc tgctgcgcgc cggcgcgcag accctcgcgc tgcatgtcga
     1321 cgggctgcgc ggcaaccagg aagtcatcgt caagaacgcc ggcccgcagc tcacccgcgt
     1381 cgtcggcatg agcggcgcaa ccgtgcttgg cgacggcgag atcgtgctga tcctcaaccc
     1441 ggtcgcgctc ggcgcgcgcg ccgccaccac cacggacgag gcgcacgcgg ggttgttcgg
     1501 tcccgctgcc gaggcgccgc cgccgctgca cctgcccacg gtgatggtgg tggacgactc
     1561 gctgaccgtg cgcaagatca ccggtcgcct gctcgagcgc gagggctatc gcgtgctcac
     1621 cgccaaggac ggcgtggacg cgctcgagca gctcatcgac ctgaagcccg acgtggtgct
     1681 caccgacatc gagatgccgc gcatggacgg cttcgacctc gtccgcaaca tccgcgccga
     1741 cgcgcgcctg cacgacctgc cggtggtggt gatcacctcg cgcctggcgg agaagcaccg
     1801 ccgttacgcc ctggaggtcg gcgccgacca ctacctcggc aagccctacc aggaagagga
     1861 gctgctcgcg ctggtggcgc gctacgcaca ggcgggcaag ggctgagggg ggcctcgctc
     1921 tttctgcggt gccagccggg atgcggcaac ggatgcgcct cgacgccctc cgaaccgccg
     1981 ccgatctcga cttgcgtcgc gcctgcgccc ccggcgtgga agcacgctgc aggagcgacg
     2041 caagtcgaga tattgcagtg gaagaccgct ccgtcccggc gtctacgcgc ccagcgcgcg
     2101 cgccatgcgc gcgagcgctt cgtccagcgt cgcgcgcggg cagccgaagt tgagccgcac
     2161 gaagcccggc gcgccgaagg ccgcgccatc cgagagcccg acgccggcgg cctcgaagaa
     2221 ggccgccggg tcggagaccc cgcgcgccgc catcatcgcg cggcagtcga cccaggccag
     2281 gtaggtcgct tcggggcgcg tcgtcgtcag gccgggcatc gcacccagca cctccagcac
     2341 acgctcgcgg ttgccgcgca ggtaatccag cagcgcccgt cgccaggcat cgccgtcgcg
     2401 gtaggcggcc tccgccgcga ccatgccgag cacgttcaca tgcggcacga tgccgcgcat
     2461 ggcgtggcgg tagcgccggc gcagcgcggg gtcgggaatg atcgcgaagg cgcagtacag
     2521 cgccgggatg ttccaggtct tggacggcgc catcagggtg atcgtgcgcc gcgcgatcgc
     2581 ctcgtcgagc gcggcgagcg ggcggtgcgt gcgatcggcg tcgagaacca ggccgcagtg
     2641 gatctcgtcc gagcacacca gcaggtcgtg gcgcgcggcg aagtcggcga gccggcgcag
     2701 ttcggcttcg tcgaacaccc ggccgaccgg gttgtgcggg ctgcacagca gcagcagccg
     2761 ggtcgtgggc gtgcaggcgg cctcgagcgc gtcccagtcc cattgccagc ggccgtcgcc
     2821 cagcaccagt tcggcgcgct gcagcacgcg gccggtgttg gccggggcgg taaggaaagg
     2881 cggatacacc ggggtggcgg tgagcacgcc gtcgcccgct tcgccggcga tggcgcaggc
     2941 gaggttgaag ccggcgacca cgcccggcag ccacaccagc cagtcgggct ggatcgtcca
     3001 tccgtgatcc cgccgcaggc cttcgaccac ggcctgctcg agcgcgggcc aggcatcggt
     3061 gtagccgaac acaccatggt cgatgcgccg gtgcagggcc tcgatgaccg ccggcggcgc
     3121 ggcgaagtcc atgtcggcga cccacagcgg cagcacgtcg cggccggcgt agcggcccca
     3181 tttttcgctc ggcaggctgc ggcggtcgat gaggcggtcg aagtcgaagg cgggggcggt
     3241 catggcgggg cgtccggtga agggtgacgg ggcgtcattg tcccgcattc ccccgagcgc
     3301 tcgctgcccc gcgggagcga cgcaagtcgc gatccggcgc ccggacggcg caccgaatcc
     3361 ccgggctgac gcctatgtgc cttgcgcgtc gttcttcacc acgccgaaac gttgcagcgt
     3421 gcgctcacgc gcagcggcgt gatccacgat cggtccgggg taatcgacgc cgatcttcgt
     3481 ccggcacgcg gtctggtcga tgccgcccat ggtcca
//
LOCUS       DAOQNN010000342         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18169_length_3510_cov_12.613314, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000342 DAOQNN010000000
VERSION     DAOQNN010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18169_length_3510_cov_12.613314"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1041)
                     /locus_tag="PKV97_14175"
     CDS             complement(136..1041)
                     /locus_tag="PKV97_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07076.1"
                     /translation="MSTDGSDETTARLKHLGGVEILVLVLALGTGGLAAAYAIAMAVG
                     AHAAGLEPPLMLLELGGILALGEGGALVVGVVLFGLAGLRYWDNGFRLDARVVAMPLA
                     IAVIVWSVKYAHEAREAGAARHAREMAEAAEQGGKQRLREIAFACHGFCGYAEDSGPL
                     VWLVGQADSVADYNAIGLMLNQRADRSARSGRMSDPRSALEVAVDRYDTQPDLVAYLL
                     GISRVNHHFMVTRASVAEFDATLLYAVKRRAGAPLVSLLVGNGANPNADVDGGATALE
                     ASAWYPDGEVAQAVRRALNGGNGSR"
     gene            complement(1076..2443)
                     /locus_tag="PKV97_14180"
     CDS             complement(1076..2443)
                     /locus_tag="PKV97_14180"
                     /inference="COORDINATES: protein motif:HMM:NF025433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HNB07077.1"
                     /translation="MKAVPALLRGVVAVGALVALAGCAGPSGAMRSGAATEPALQSMA
                     QDGVTVSVAVLTAAQARERFGVDLDAHGVQAAWIRVRNASPRGLWFIRNLLDPDFYSA
                     DEVASMTEGEVPNAGREAVRQHLRDESIRVFMQPAAITEGHLFLPKMEGGRYLDLRLQ
                     GDAWDEAQAAVTAPARPRELRFGFAVAMPDGEFDFEQLHPAKIYAGRETPDVDAAGLR
                     RALEALPCCTSDRGGSNPGDPLNVVLVGEAAEVMHALTRGGWSFTHRITAHTVGREIA
                     AALSGSAYAVAPVSSLYAFDRSHDVALQRARRSIAQRSHMRLWLAPFLFEGRHVWVGQ
                     VSRDIGVKLTTKSPTLTTHVIDPEVDATREYLLHSLMARNLVERFGFVKGSAAAPREA
                     PRRNLTDDPYFSDGLRLVVVLAQEAVAPGAARNLMWERSAAPIAEGQSPAAQRNVRPI
                     GAAAE"
     gene            complement(2440..>3510)
                     /locus_tag="PKV97_14185"
     CDS             complement(2440..>3510)
                     /locus_tag="PKV97_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07078.1"
                     /translation="AGCAPQAMLGYRPEAPISANLPLGAAGVRDARAAFAPLFERELR
                     AFDASADAAAWLHTGPAAQRDVSMALEEIDRRFDERRVQTVVLVVPGLLGDCVDDQSV
                     PFGDGVLRTRELESTASYAQYADLGLRDIRLLWVPGRASVEANGARLAAALREVAARD
                     GVAHIVLVAYSKGTPDVLHALRLLEADGGVPRQIAALVSVAGIVMGTPLADHYETLYA
                     SLSPLLSPFGCTAAAGGEVASLTRRERADWLAAHPPPPALAYHSVVAFAAPDETAPFL
                     RHSQAMLAAIDPRNDGQVVAADALLPGSALIAAARADHWSLALPLERNPHALVRAVAP
                     SRTFPRAPLFRALVKWVVGTLP"
BASE COUNT          517 a         1360 c         1175 g          458 t
ORIGIN      
        1 gctgcatgcc gcgattcagc gaggccgggc tggagttcca ggtctgggat gcccggaccc
       61 ggacgcacat ctacaaatcg gaatcggcct tcctgcccga tagccggcca cccgtgcggt
      121 agcggcggca cccgctcacc gcgagccgtt gccgccgttc aacgcgcgcc tgaccgcctg
      181 cgccacttct ccgtccgggt accacgccga cgcttcgagc gccgtagcgc cgccatcgac
      241 atctgcattc ggattggcgc cgttgccgac cagcaggctg accagcggcg cacccgcgcg
      301 acgcttcacg gcgtagagca gcgtcgcgtc gaattcggcc acactcgcgc gcgtcaccat
      361 gaagtggtgg ttgacgcggc tgatgccgag cagataggcc accagatccg gctgggtgtc
      421 gtaccggtcc accgccactt ccagggccga gcgcgggtcg gacatccggc ccgaacgcgc
      481 gctgcggtcg gcgcgctggt tcagcatcag gccgatcgcg ttgtagtccg cgacgctgtc
      541 cgcctggccg accagccaca ccagcgggcc gctgtcctcg gcatagccgc agaagccgtg
      601 gcaggcgaac gcgatctcgc gcaggcgctg ctttccgccc tgttccgccg cctccgccat
      661 ctcgcgggcg tggcgtgccg cgccggcctc acgcgcctca tgcgcgtact tgaccgacca
      721 cacgatcacc gcaatcgcca gcggcatggc cacgaccctt gcatcgagcc gaaagccgtt
      781 gtcccagtag cgcaatccgg cgaggccgaa cagcaccaca ccgaccacca gcgccccacc
      841 ctcgccgagc gccaggatgc cgccgagttc gagcagcatc aggggcggct ccagcccggc
      901 tgcatgcgcc ccgactgcca tggcgatcgc ataggccgcg gccaggccgc cggtgcccag
      961 ggcgaggacg aggaccagta tctcgacgcc gcccaggtgc ttcaggcgcg ctgtcgtctc
     1021 gtcggaaccg tcagtactca tgggtcatcc gtccgatccg cctgtcgccc cggcatcatt
     1081 cagccgccgc cccgatcggc cgcacgttgc gctgcgcggc cgggctttgt ccttcggcga
     1141 tgggggcggc cgagcgctcc cacatcaggt tgcgcgcggc gcccggcgcg accgcctctt
     1201 gggcaagcac gaccaccagg cgcaggccgt cgctgaaata cggatcgtcg gtcaggttgc
     1261 gccgcggcgc ctcgcgcggg gccgccgcgc tgcccttgac gaaaccgaag cgctcgacca
     1321 ggttgcgcgc catcaagctg tgcagcaggt attcgcgcgt cgcgtcgacc tcggggtcga
     1381 tcacgtgcgt cgtcagcgtc ggcgacttgg tggtgagctt cacgccgatg tcgcggctca
     1441 cctgccccac ccacacatgg cgcccctcga acaggaacgg cgccagccac agccgcatgt
     1501 ggctgcgctg cgcgatcgag cgccgtgcgc gctgcagcgc cacgtcatgg ctgcggtcga
     1561 aggcatacag gctgctcacc ggcgccaccg cataggccga tccgctcagc gccgcggcga
     1621 tctcgcggcc gacggtgtgc gcggtgatcc ggtgcgtgaa cgaccagccg ccgcgcgtca
     1681 gcgcatgcat cacctcggcc gcctcgccca ccagcaccac gttgagcggg tcgcccgggt
     1741 tgctgccgcc gcggtccgag gtgcagcacg gcagcgcttc gagcgcgcgg cgcaggccgg
     1801 ccgcgtcgac atccggcgtc tcgcgcccgg cgtagatctt cgccgggtgc aactgttcga
     1861 agtcgaactc gccatcgggc atcgcaacgg cgaagccgaa gcgcagctcg cgcggccgcg
     1921 ccggcgcggt gacggcggct tgcgcctcgt cccacgcatc gccctgcagg cgcaggtcga
     1981 ggtagcggcc gccttccatc ttcggcagga acagatggcc ctcggtgatt gccgccggct
     2041 gcatgaacac gcggatcgac tcgtcgcgca ggtgctgccg cacggcctcg cgcccggcat
     2101 ttggcacctc gccttcggtc atcgacgcca cctcgtcggc cgagtagaaa tccgggtcga
     2161 gcaggttgcg gatgaaccac aacccgcgcg gcgaggcgtt gcgcacgcgg atccacgctg
     2221 cctgcacgcc gtgcgcatcg agatcgacgc cgaagcgctc gcgcgcctgc gcggccgtca
     2281 gcacggcgac cgagacggtg acgccgtcct gcgccatcga ctgcaaggcc ggttcggttg
     2341 ccgcgccgct tcgcatcgca cccgacggcc ccgcgcagcc ggccagcgcg accagcgcgc
     2401 ccacggcgac aaccccccgc agcaaggcgg gcacagcctt catggcaacg tgcccacgac
     2461 ccacttcacg agggcgcgga acagcggcgc acgcggaaac gtgcggctcg gcgccaccgc
     2521 gcgcaccagc gcgtgcgggt tgcgctccag cggcagcgcg aggctccagt ggtccgcgcg
     2581 cgccgcggca atcagcgcgc tgcccggcag cagcgcatcc gccgcgacca cctgcccgtc
     2641 attgcgcggg tcgatggccg ccagcatcgc ctggctgtgg cgcaggaacg gcgccgtctc
     2701 gtccggcgcg gcgaaggcca ccacggaatg gtaggccagc gcgggtggcg gcgggtgcgc
     2761 ggccagccag tccgcgcgtt cgcgccgcgt gaggctcgcc acctcgccgc cggccgccgc
     2821 cgtgcagccg aacggcgaca gcagcggcga caggctcgcg tagagcgtct cgtaatggtc
     2881 ggccagcggc gtgcccatca cgatgccggc caccgacacc agcgcggcga tctgccgcgg
     2941 cacgccgccg tccgcctcca gcaggcgcag cgcgtgcagc acgtcgggcg tgcccttcga
     3001 gtaagcgacc agcacgatgt gcgcgacccc gtcgcgcgcc gccacctcgc gcagcgccgc
     3061 cgccagccgc gcaccgttgg cctcaaccga cgcacgcccc ggcacccaca gcaggcggat
     3121 gtcgcgcagg ccgaggtcgg cgtactgcgc gtacgacgcc gtcgattcca gctcgcgcgt
     3181 gcgcagcacg ccgtcgccga agggcaccga ctggtcatcc acgcaatcgc ccagcaggcc
     3241 gggcaccacc agcaccacgg tctgcacccg ccgctcgtca aagcggcggt ctatctcttc
     3301 gagcgccatc gatacgtcgc gctgtgccgc cgggcccgta tgcagccacg ccgccgcatc
     3361 ggccgacgca tcgaaggcgc gcaactcgcg ttcgaacagc ggcgcgaacg ccgcgcgcgc
     3421 atcgcgcacg cccgccgcgc caagcggcag gttcgcgctg atcggcgcct cgggccgata
     3481 gcccaacatc gcctgcggcg cacagccggc
//
LOCUS       DAOQNN010000343         3509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18179_length_3509_cov_11.623625, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000343 DAOQNN010000000
VERSION     DAOQNN010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18179_length_3509_cov_11.623625"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PKV97_14190"
     CDS             complement(<1..1318)
                     /locus_tag="PKV97_14190"
                     /inference="COORDINATES: protein motif:HMM:NF021128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HNB07079.1"
                     /translation="MKIHDLIQGSPEWLAYRAQHFNASDAPAMMGCSPYKTRAQLLRE
                     MHTGVAPDVDIGTQKRFDNGHRAEALARPLAEEFIGAELYPVTGSEGKFSASFDGLTL
                     DEGTGFEHKAMNDDLRACIVDEDSGAALPLHHRVQMEQQLLVSGAERILFMTSAWTPD
                     GDLIEERHCWYYSEPALRDKIIAGWRQFERDLAEYVPAAAADPAPIGKAPETLPALRI
                     EVTGAVTASNLAEFRETALAAIRSVNRTLKTDQDFADAEKAIKWCAEVESRLKAAKDH
                     ALSQTSSIDALFKALDDIGTEARTVRLDLDKLVARRKSEVKEEAVAAARRALEQHVAA
                     INAEIAPMRLPPVPADFGGAIKGLRSVASMDDKLQSLLASAKVACDALARGIRANVAA
                     FKEQAAGYEFLFVDLGQIVHKAPEDFAATLQARIAAHKEAEAARERQ"
     gene            complement(1315..2184)
                     /locus_tag="PKV97_14195"
     CDS             complement(1315..2184)
                     /locus_tag="PKV97_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07080.1"
                     /translation="MNELQTTPAPTAMAAAFDLSPRTFEQALQLADYMAKSEMVPKQY
                     RGRPGDCFIAMQWGMEIGLKPLQALQSIAAINGKPNIYGDAGKALLLAHGCIIEEDDA
                     ETVKANSRARCCIKRPGRPPVQRTFSLEDAKTAGLWNKEGPWRAYPWRQMAWRAFWFA
                     ARDAAADLLRGMGGMEEAMDTPTERHMGMAEEVAPPAAPALPDYAADAFDKNLPLWAK
                     LVADGKKTAPDLLATLSTKATFSEEQKTRILSLKKAAATPAPPPPTDDAPDDGHDDFR
                     AGLDATTTEGSGK"
     gene            complement(2190..2381)
                     /locus_tag="PKV97_14200"
     CDS             complement(2190..2381)
                     /locus_tag="PKV97_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07081.1"
                     /translation="MIADPNEGPRTCGWCEHLMRCPTPQACELAEEERRRRSSRRDLI
                     AAVALLLAVLGTLALINWR"
     gene            complement(2378..3307)
                     /locus_tag="PKV97_14205"
     CDS             complement(2378..3307)
                     /locus_tag="PKV97_14205"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNB07082.1"
                     /translation="MRVLDLFSGAAGGWSLGLHRAGFTTVAACEIDEQRRAAYAHNNP
                     GVRLYDDVRSLTAGRLLADLGYLPECVAGSPPCQDASTANSKGRGIDGERTGLFRDFV
                     RIVGEVRPRWVAAENVPGLRTRGADWVLDALEARGYTCWPLVVGAVHAGAPHERKRAW
                     FIAADADQVGRPHARKQRARQGKPRRTIAAHSAANNLHADQDSKPHMPVDGEVARGMG
                     LPGDADEAVLEIGQGERRDFRSQLAALERAVGPLGARWHGGAARSFGMAAGLPAGLAR
                     QCVAAYGDSVLPQITEAIGHAIRRTEEALRAAT"
BASE COUNT          451 a         1213 c         1236 g          609 t
ORIGIN      
        1 gctgccgctc gcgcgccgcc tcggcctcct tgtgcgccgc gatgcgcgcc tgcagcgtgg
       61 ccgcgaagtc ctccggcgcc ttgtgcacga tctggcccag gtcgacgaac aggaactcgt
      121 agccggcggc ctgctccttg aacgcggcca cgttcgctcg gatgccgcgc gccagcgcgt
      181 cgcaggcgac cttggccgac gccagcagcg actggagctt gtcgtccatg ctggccaccg
      241 agcgcaagcc cttgatggcc ccgccgaagt cggccggtac cggcggcagc cgcatcggcg
      301 cgatctcggc gttgatcgcc gcgacgtgct gctcgagcgc gcgccgcgcg gccgcgacag
      361 cctcctcctt cacctcgctc ttgcgccggg cgaccagctt gtccaggtcc aggcgcacgg
      421 tgcgcgcctc ggtgccgatg tcgtcgagcg ccttgaagag cgcgtcgatg ctgctggtct
      481 gcgacagcgc gtggtccttc gcggccttca ggcgcgactc cacctcggcg caccacttga
      541 tcgccttctc ggcgtcggcg aagtcctggt cggtcttcag cgtgcggttg accgagcgga
      601 tcgcggccag cgcggtctcg cggaactcgg ccaggttgct cgcggtgacg gcgccggtca
      661 cttcgatgcg cagcgcgggc agggtctcgg gggccttgcc gatcggcgcg gggtcggccg
      721 ccgcggcggg gacgtactcg gccaggtcgc gctcgaactg gcgccagccc gcgatgatct
      781 tgtcgcgcag cgccggctcg gagtagtacc agcagtgacg ctcctcgatc aggtcgccgt
      841 cgggcgtcca ggcgctggtc atgaacagga tgcgctcggc gccgctcaca agcagctgct
      901 gctccatctg cacgcggtgg tgcagcggca gcgcagcacc ggaatcctcg tcgacgatgc
      961 acgcccgcag gtcgtcgttc atcgccttgt gctcgaagcc ggtgccctcg tcgagcgtga
     1021 gcccgtcgaa ggacgcggag aacttgccct cgctgcccgt caccgggtac agctcggcgc
     1081 cgatgaactc ctcggccagc gggcgcgcca gcgcttcggc gcggtggccg ttgtcgaagc
     1141 gcttctgcgt gccgatgtcg acgtcgggcg cgacgccggt gtgcatctcg cgcagcagct
     1201 gcgcccgcgt cttgtagggg ctgcagccca tcatcgcggg cgcgtcgctg gcgttgaagt
     1261 gctgggcgcg ataggcgagc cactccgggc tgccctggat caggtcgtgg atcttcactt
     1321 gccgctccct tcggtggtgg tggcgtccag gcccgcgcgg aagtcgtcgt gaccgtcgtc
     1381 gggcgcatcg tcggtcgggg gcggcggcgc cggcgttgcg gcggccttct tcagcgacag
     1441 gatgcgagtt ttctgctcct cgctgaacgt ggccttggtg ctcagcgtgg ccagcaggtc
     1501 gggcgcggtc ttcttgccgt cggcgacgag cttcgcccac agcggcaggt tcttgtcgaa
     1561 ggcgtcggcc gcgtagtcgg ggagcgcggg agcggccggg ggcgcaactt cctcggccat
     1621 gcccatgtgg cgctcggtcg gcgtgtccat cgcctcttcc atgccgccca tgccgcgcag
     1681 caggtcggcc gcggcatcgc gcgcggcgaa ccagaacgcg cgccaggcca tctggcgcca
     1741 cgggtacgcg cgccacgggc cttccttgtt ccacaggccg gcggtcttcg cgtcctcgag
     1801 cgagaacgtg cgctgcaccg gcgggcggcc cgggcgcttg atgcagcagc gcgcacggct
     1861 gttcgccttg acggtctctg cgtcgtcctc ttcgatgatg cagccgtggg ccagcagcag
     1921 cgccttgccg gcgtcgccgt agatgttggg cttgccgttg atggccgcga tggactgcag
     1981 cgcctggagc ggcttcagcc cgatctccat gccccactgc atggcgatga agcagtcgcc
     2041 gggccggccg cggtactgct tgggcaccat ctcggacttg gccatgtagt cggcgagctg
     2101 cagcgcctgc tcgaaggtgc gggggctcag gtcgaaagcg gctgccatcg cagtcggcgc
     2161 gggagtggtc tgaagttcgt tcacggggtt cacctccagt tgatgagtgc aagggtgccg
     2221 agcactgcga gcagcagcgc cacggcggcg atcaggtcgc gccgggagga ccggcggcga
     2281 cgctcttctt cagcgagctc gcacgcctgc ggcgtcgggc agcgcatcag gtgctcgcac
     2341 cagccgcagg tgcgcgggcc ttcgttgggg tcggcgatca tgtggcggcc ctcagcgctt
     2401 cttccgtccg ccgaatcgcg tgcccgatgg cttcggtgat ctgcgggagg accgagtctc
     2461 cataggcggc cacgcattgg cgagccagcc cggcgggtag ccctgcagcc attccgaaac
     2521 tgcgtgctgc gcctccgtgc catcgagccc caagcggacc aactgctcgt tcaagggccg
     2581 cgagttgcga tcgaaagtcg cgtcgctcgc cttgcccgat ctccagtacc gcttcgtcgg
     2641 cgtcgccagg aagccccatg ccccgcgcca cttctccatc gacgggcatg tgaggtttgc
     2701 tgtcttggtc ggcgtgaaga ttgttcgcag cactgtgtgc agcgatggtc cgtcttggct
     2761 tgccttgccg ggcccgctgt ttccgcgcat gcggccgtcc gacttgatcg gcgtcggcag
     2821 caatgaacca ggcgcgcttt cgctcgtgcg gagctccggc atggacagca cccaccacga
     2881 gcggccagca ggtgtaaccg cgcgcctcca gtgcatcgag aacccagtcc gcgcctcgag
     2941 ttctgagacc agggacattc tcagcagcga cccaacgagg gcggacctcg ccgacgatgc
     3001 ggacgaagtc tctgaacagg ccggtgcgct cgccgtcgat gccgcggccc ttgctgttcg
     3061 cggtgctggc gtcctggcag ggcgggcttc cggcgacgca ctcggggagg tatccgagat
     3121 cggccaaaag tcggcctgcg gtgagtgatc gcacatcgtc atacaggcgc acgcccgggt
     3181 tgttgtgcgc gtaggccgcg cgtcgctgtt cgtcgatctc gcacgcggcg acggtggtga
     3241 agccggcgcg gtgcaggccg agggaccatc caccggcggc gccgctgaac aggtcgagca
     3301 cgcgcatcac gcccccaccc aagtcgccac ctcagcgagc tcgcacgcct gcggtgtcgg
     3361 gcacgcgtcc aggcgccagc atcggctgca caggcggcct tcgttcgggt cggcgatcac
     3421 ggggcggcct ccagcagcgc gcggtcgtca gacatcggcg gtctccttcg gcttcatgaa
     3481 caccatccag tgcgtgcctg ccttcttgc
//
LOCUS       DAOQNN010000344         3497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18264_length_3497_cov_7.311156, whole genome shotgun sequence.
ACCESSION   DAOQNN010000344 DAOQNN010000000
VERSION     DAOQNN010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18264_length_3497_cov_7.311156"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..956
                     /locus_tag="PKV97_14210"
     CDS             24..956
                     /locus_tag="PKV97_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNB07083.1"
                     /translation="MAPAAGDTSVPGVAAAAAAFVPRPAVRSAFAERSMILDATRAGP
                     RLVAVGERGVVLLSDDDGAHWRQAGKVPVDATLTAVSFADAREGWAVGHLGVILHSAD
                     GGETWSVQRIDPVEDRPLFAVAFTDARNGVAVGLWSLMLRTRDGGRTWEAAELPPPPG
                     DTRADANLMSVFGDGQGRLFIAGERGLVLRSLDGGATWSYHPTGYRGTFWSGTALADG
                     TLIVGGLRGSVYRSTDNGEHWQRVETGSQSSVTDLVAFDGRVHAVGLDGVSLDSRDGG
                     ASFAVERRADRLSLTAAVATAGGVLHFSRAGLVR"
     gene            complement(1380..1787)
                     /locus_tag="PKV97_14215"
     CDS             complement(1380..1787)
                     /locus_tag="PKV97_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB07084.1"
                     /translation="MSDTLQPGLSLTRKYDIDAARTIAFMGDECRVYSTPALLYDIEV
                     CCRDLLLQHIGEGKDSVGTRVELDHVGATLLGMWIEITVKLVQVDGPAVVFEFSARDA
                     LEEVARGRHNRFVVGVDKTRQRLLGKLAKAQAA"
     gene            complement(1852..>3497)
                     /locus_tag="PKV97_14220"
     CDS             complement(1852..>3497)
                     /locus_tag="PKV97_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNB07085.1"
                     /translation="LPDAPGAQAALQELALDCACDGEAVRVVAARCSVGAPPPLRVAR
                     PIHKIDRLLHPERIGIVGASASGMNFGRIILRNLLGSGCAPERLCVIRPGGGEIDGVA
                     CIENLAAIEGKLDLLIVAVAADAVYPLVDEIIAAGTVEAVMLIPGGLGETAKSRAPAA
                     DLAARINAAHACGDGGPIFLGANCLGVVSHPGRYDSWFIPLERLPKPQKAPERRAVML
                     SQSGAFMITRLSHNPWLDPRYMIAMGNQTDLTHGDLLSWFAAREDVDSIGIYVEGFRD
                     LDGLAFARAVRRAVLAGKQVVVYKSGRSEAGQAGVMGHTASIAGDPVLFASVLAQAGA
                     TVTDDVEVFDDLFYMAGTLHAKRIGGDRLAAASGAGFEAVSAADAIVGETFSMRMAAL
                     SADTVGRIEAVLAEKKLAALVEVRNPLDMNPGADDDAHVRITAAMIEDSEVDAVVVGL
                     DPTAPMVRALEQSRLRPGYDLSDPESTVHTLPPLVARTDKPIVAVVDAGRLYDAMAAG
                     LMDRGVCVFRNVARGTRALVRHVEARLDAERIRRHFGQS"
BASE COUNT          462 a         1174 c         1346 g          515 t
ORIGIN      
        1 gctgcgcggc gctctgcgcg agcctggcgc ccgctgccgg cgatacgtcg gtgcccggcg
       61 tggccgccgc tgccgccgcc ttcgtaccgc ggcctgcggt gcgctcggcc ttcgccgaac
      121 gcagcatgat cctcgacgcg acccgtgcgg gcccccgcct cgtcgcggtg ggggagcgcg
      181 gcgtggtgct gctctccgac gacgacggcg cgcactggcg ccaggcgggc aaggtgccgg
      241 tcgacgcgac cctgaccgcg gtgagcttcg ccgacgcgcg cgagggctgg gcggtggggc
      301 atctcggcgt gatcctgcac agcgccgacg gcggcgagac ctggagcgtg cagcgcatcg
      361 accctgtcga ggacaggccg ctgttcgctg tggcgttcac cgacgcacgc aacggagtcg
      421 cggtcgggct gtggtcgctg atgctgcgca cccgcgacgg cggccggacc tgggaggcgg
      481 cggagctgcc gccgccgccg ggcgacacgc gcgccgacgc caacctgatg agcgtgttcg
      541 gcgatgggca ggggcgacta ttcatcgccg gggagcgcgg cctggtgttg cgctcgctcg
      601 atggcggcgc gacctggagc tatcatccga ccggctatcg cggcacgttc tggagcggca
      661 ccgcgctcgc cgacggcacg ctgatcgtcg gtggcctgcg cggcagcgtc tatcgcagca
      721 ccgacaacgg cgaacactgg cagcgcgtcg agaccggctc gcagagctcg gtcaccgacc
      781 tcgtcgcctt cgacggtcgc gtgcatgcgg tcggcctcga cggtgtcagc ctcgacagcc
      841 gcgatggcgg ggcgagcttc gcggtggagc ggcgggcgga tcgcctgtcg ctgaccgccg
      901 cggtggcgac ggccggtggt gtgctgcatt tttcgcgcgc tggcctggtg cgctgaaacg
      961 ggtgggcgcg caattcaggc gccgtatcgc ggcttgcgcc gctcctacgg agaaccgctt
     1021 acgccctgta ggagcgccgc aaggcgcgaa cggtgccttg aggcggcgat gacgcgggat
     1081 ccatgcgccg tatcgcggct tgcgccgctc ctacggagaa ccgcttacgc cctgtaggag
     1141 cgccgcaagg cgcgaacggt gccttgaggc ggcgatgacg cgggatccat gcgccgtatc
     1201 gcggcttgcg ccgctcctac ggagaaccgc ttacgccctg taggagcgcc gcaaggcgcg
     1261 aacggtgcct tgaggcggcg atgacgcggg atccatgcgc cgtatcgcgg cttgcgccgc
     1321 tcctacggag aaccgcttac gccctgtagg agcgccgcaa ggcgcgaacg ctggcccgct
     1381 caggccgcct gcgccttggc gagcttgccg agcaggcgct ggcgggtctt gtcgacgccg
     1441 accacgaagc ggttgtggcg gccgcgggcg acctcctcga gggcgtcgcg cgcgctgaac
     1501 tcgaacacca ccgcagggcc atcgacctgg accagcttga cggtgatctc gatccacatg
     1561 ccgagcaggg tggcgccgac gtggtcgagc tcgacgcggg tgccgaccga gtccttgcct
     1621 tctccgatgt gctggagcag caggtcgcgg cagcacacct cgatgtcgta gagcagcgcg
     1681 ggggtggaat agacgcggca ctcgtcgccc atgaaggcga tcgtgcgggc ggcgtcgata
     1741 tcgtacttgc gggtgaggct gaggccgggt tgaagggtgt cgctcatcgt ctgtctccgt
     1801 ctgcgggtgg ttgggtgtgt gttcgtgctg cggggtgctt gcgggctgga atcacgattg
     1861 gccgaaatgg cggcggatac gctcggcgtc caggcgggcc tcgacgtggc gcaccagcgc
     1921 gcgggtgccg cgcgcgacgt tgcggaacac gcatacgccg cgatccatca ggccggcggc
     1981 catggcgtcg tagaggcggc cggcgtcgac gaccgcgacg atcggcttgt ctgtgcgcgc
     2041 caccagcggc ggcagggtgt gcaccgtgct ctcggggtcc gacaggtcgt agccggggcg
     2101 cagcctgctc tgttcgagcg cgcgcaccat gggggcggtg gggtcgaggc cgaccacgac
     2161 cgcgtcgacc tcggaatcct cgatcatcgc ggcggtgatg cgcacgtggg cgtcgtcgtc
     2221 ggcgcccggg ttcatgtcga gcgggttgcg cacctcgacc agggcggcga gcttcttctc
     2281 ggccagcacc gcctcgatgc ggccgacggt gtcggcggac agcgcggcca tgcgcatcga
     2341 gaaggtctcg ccgacgatcg cgtccgcggc gctcaccgcc tcgaagccgg cgccgctcgc
     2401 cgcggcgagg cggtcgccgc cgatgcgctt ggcgtgcagg gtgccggcca tgtagaagag
     2461 gtcgtcgaag acctcgacgt cgtcggtgac ggtggcgccg gcctgggcga gcaccgaggc
     2521 gaacagcacc gggtcgcccg cgatcgaggc ggtgtggccc atcacgccgg cctggccggc
     2581 ctcgctgcgg cccgacttgt acaccacgac ctgcttgccg gcgagcaccg cgcgccgcac
     2641 cgcgcgcgcg aaggccaggc cgtcgaggtc gcggaagcct tcgacgtaga tgccgatgct
     2701 gtcgacgtcc tcgcgcgcgg cgaaccagga cagcaggtcg ccgtgggtga ggtcggtctg
     2761 gttgcccatc gcgatcatgt agcgcgggtc gagccagggg ttgtgcgaga gccgggtgat
     2821 catgaaggcg ccgctctggc tcagcatgac cgcgcggcgt tcgggtgcct tctgcggctt
     2881 gggcaggcgc tcgagcggaa tgaaccagga gtcgtagcgc cccgggtgtg agaccacgcc
     2941 gaggcagttg gcaccgagga agatcggccc gccgtcgccg cacgcgtgcg cggcgttgat
     3001 gcgggccgcc aggtcggcgg cgggcgcgcg gctcttcgcg gtctcgccga ggccgcccgg
     3061 gatcagcatc acggcctcga cggtgccggc cgcgatgatc tcgtcgacca gggggtagac
     3121 cgcgtcggcg gcgaccgcga cgatcagcag gtcgagcttg ccctcgatcg cggcgaggtt
     3181 ctcgatgcag gcgacgccgt cgatctcgcc gccaccgggg cggatcacgc acaggcgctc
     3241 gggcgcgcag ccgctgccga gcaggttgcg caggatgatg cgaccgaagt tcatgccgct
     3301 ggccgaagcg ccgacgatgc cgatgcgctc ggggtggagc aggcggtcga tcttgtggat
     3361 tggtcgtgcc acgcgcagcg gcggaggggc gccgacgctg cagcgcgccg cgacaacccg
     3421 gacagcctcg ccgtcgcagg cgcagtccag cgccagctcc tgcagcgcgg cctgcgcgcc
     3481 cggggcgtcg ggcaggc
//
LOCUS       DAOQNN010000345         3495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18286_length_3495_cov_11.491860, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000345 DAOQNN010000000
VERSION     DAOQNN010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18286_length_3495_cov_11.491860"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PKV97_14225"
     CDS             <1..676
                     /locus_tag="PKV97_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB07086.1"
                     /translation="VVLADLNEEAGKALAAELGAAARFVRTDVTDEASTQAAVEAAVQ
                     GCGGLHGLVCCAGVAPGEKVVGKDGPHGLDRFVRGVMINLVGTFNPIRLAAAAMSKAA
                     PNEEGERGVIVTTASVAAFDGQIGQATYAASKAGVVGMTLPIARELARYGIRVMSIAP
                     GIFETPMLRGLPQAVQDSLGEMVPFPSRLGRASEYAQLVRSICENPMLNGEIIRLDGA
                     IRMAAK"
     gene            687..2255
                     /locus_tag="PKV97_14230"
     CDS             687..2255
                     /locus_tag="PKV97_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNB07087.1"
                     /translation="MNPVHTTMIPADVRRAGRNLIGDAFARSVRRNPEREALRFEGRS
                     WRYAQLDRAANRVANRLLGLGLAVGDRVAAYGRNSDAYVLLWLGCVKAGLIHVPINYA
                     LLDAELRYIVEQSGARALFCDADMATHVEALGSDLPCEWRGTLHGGHERDVLEWAQAG
                     GTDTAPAIALDEEAIAQLLYTSGTTAAPKGAMMSHRALLAEYTSTLLATDIRAEDCAL
                     AALPLYHSAQMHVFLMPQLLVGATTLLIQAPQPERCFELIERERVTSFFAPPTVWIAF
                     LRHPAFAPARLASLQKGYYGASIMPVPVLQELAARLPALQLYNCYGQSEIAPLATVLR
                     PEEHAERPASAGRPIFNVETRIVDSDLNDVPAGEMGEIVHRSPQLMSGYWDKPEETAA
                     SFRDGWFRSGDVGYLDEAGYLYITDRIKDIIKTGGVVVASREVEECLYTHPAVAEVAV
                     IGLPDERWIEAVTAVVALKQGAQATAAELIAHVHARLAAFKVPKRVFFVEDMPRNASG
                     KLLKRELRVRYAET"
     gene            complement(2290..2781)
                     /locus_tag="PKV97_14235"
     CDS             complement(2290..2781)
                     /locus_tag="PKV97_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNB07088.1"
                     /translation="MNITQHTDYALRLLMYLGTCTERRVTIKEVAERFDISRSHLMKV
                     VTGLVAKGFVDGARGKGGGLRLARAATQIGLGDVVRGMEPSFELVECFGSGSHCLLDP
                     ACRLKGALAGALQAFFAALDRLTLADLIGDDGQLHAVLGIPQADAQAPRPTIEIRPGT
                     RAD"
     gene            2901..3257
                     /locus_tag="PKV97_14240"
     CDS             2901..3257
                     /locus_tag="PKV97_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNB07089.1"
                     /translation="MKRHPHLVRLSREHHAALRLGRHLLAGGAAAELRAEHVALVTHF
                     AEEERELAPLLETGGHGALAARLRAEHAHLRSLFATAADGVREAAAGQALIDHVRFEE
                     RELFPAIETLFEEVLP"
     gene            3254..>3495
                     /locus_tag="PKV97_14245"
     CDS             3254..>3495
                     /locus_tag="PKV97_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNB07090.1"
                     /translation="MTHVVTEACIRCKYTDCVSMCPVDAFREGPNFLVIDPEECIDCT
                     LCVAECPVDAIVPEDELSDEQREYLALNAALAKDWPR"
BASE COUNT          531 a         1229 c         1208 g          527 t
ORIGIN      
        1 ggtggtgctc gccgacctca acgaagaggc cggcaaggcg ttggcggccg aactcggcgc
       61 ggcggcccgt ttcgtgcgca ccgacgtcac cgacgaggcc tcgacgcagg cggcggtcga
      121 ggccgcggtg cagggctgcg ggggcctgca cggcctggtg tgctgcgcgg gcgtggcgcc
      181 gggcgagaag gtggtgggca aggacggccc gcatggtctc gaccgcttcg ttcgcggcgt
      241 gatgatcaac cttgtcggca ccttcaaccc gatccgcctc gccgccgccg cgatgagcaa
      301 ggccgcgccc aacgaggagg gcgagcgtgg cgtgatcgtc accaccgcct cggtcgccgc
      361 cttcgacggc cagatcggcc aggcgacgta tgcggcgtcc aaggccggcg tggtcggcat
      421 gacgctgccg atcgcgcgcg agctggcacg ctacggcatc cgcgtgatga gcatcgcccc
      481 cggcatcttc gagaccccga tgctgcgcgg gctgccgcag gcggtgcagg attcgctcgg
      541 cgagatggtg cctttcccct cgcgcctcgg ccgcgccagc gaatatgcgc agctggtgcg
      601 ctcgatctgc gagaacccga tgttgaacgg cgagatcatc cgtctcgatg gcgcgatccg
      661 catggcagcc aagtgaggag cccggcatga accccgtaca cacgaccatg attcccgccg
      721 acgtccgtcg tgccggacgc aacctgatcg gcgacgcctt cgcgcgctcg gtgcgccgca
      781 accccgagcg cgaggcgctg cgcttcgagg ggcgcagctg gcgctacgcc cagctcgacc
      841 gtgccgccaa ccgcgtcgcc aaccgcctgc tcggcctcgg cctggcggtc ggcgatcgcg
      901 tcgccgccta tgggcgcaat tccgacgcct acgtgctgct gtggctgggc tgcgtgaagg
      961 cggggctgat ccacgtcccg atcaactacg cgctgctcga cgccgagctg cgctacatcg
     1021 tcgagcagtc gggcgcgcgt gcgttgttct gcgacgccga catggcgacc cacgtcgagg
     1081 ccctcggcag cgacctgccc tgcgaatggc gcggcacgct gcacggcgga cacgagcgcg
     1141 acgtcctcga gtgggcgcag gccggcggca cggacacggc cccggcgatc gcgctcgacg
     1201 aggaggccat cgcgcagctg ctgtacacct cgggcaccac cgccgcgccc aagggcgcga
     1261 tgatgagcca tcgtgcgctg ctcgccgaat acaccagcac gctgctcgcc accgacatcc
     1321 gcgccgagga ctgcgcgctc gccgcgctgc cgctgtacca ctcggcgcag atgcacgtct
     1381 tcctgatgcc gcagctgctg gtgggcgcga ccaccctgct gatccaggcg ccgcagcccg
     1441 agcgctgctt cgagctgatc gagcgcgagc gcgtgacgtc cttcttcgcc ccgcccacgg
     1501 tgtggatcgc cttcctgcgt catccggcct tcgcgccggc gcggctggcc tcgctgcaga
     1561 agggctacta cggcgcctcg atcatgccgg tgccggtgct gcaggagctg gccgcacgcc
     1621 tgccggcgct gcagctctac aactgctatg gccagagcga gatcgccccg ctcgccaccg
     1681 tgctgcgtcc ggaggaacac gccgagcgcc ccgcgtccgc cgggcggccg atcttcaacg
     1741 tcgaaacccg catcgtcgac agcgacctca acgacgtgcc tgcgggcgag atgggcgaga
     1801 tcgtgcaccg ctcgccgcag ctcatgagcg gctactggga caagccggag gagaccgcgg
     1861 ccagcttccg cgacggctgg ttccgttcgg gggacgtcgg ctacctcgac gaggccggct
     1921 acctctacat caccgaccgc atcaaggaca tcatcaagac cggcggcgtg gtggtggcga
     1981 gccgcgaggt ggaggagtgc ctgtacaccc accccgcggt ggccgaggtc gcggtgatcg
     2041 gcctgcccga cgagcgctgg atcgaggccg tgacggcggt ggtggcgctc aagcagggcg
     2101 cgcaggccac ggccgcagag ctgatcgccc acgtgcatgc ccggctggcg gccttcaagg
     2161 tgcccaagcg ggtgttcttc gtcgaggaca tgccgcgcaa cgcctccggc aagctgctca
     2221 agcgcgagct gcgcgtgcgc tacgcggaaa cctgagcgcc ccgggtggcg gtacccgtgc
     2281 cgactcgcct cagtctgccc gggtgcccgg cctgatctcg atcgtcggcc ggggggcttg
     2341 cgcgtccgct tgcgggatgc cgagcacggc gtgcagctgc ccgtcgtcgc cgatcaggtc
     2401 ggcgagcgtg aggcggtcga gggcggcaaa gaaggcctgc agggcgccgg ccagtgcgcc
     2461 cttgagccgg caggccggat ccagcaggca gtgcgatccc gatccgaagc actcgaccag
     2521 ctcgaagctg ggctccatcc cgcggaccac gtcgccgaga ccgatctgcg tcgccgcacg
     2581 cgccagccgg agtccgccgc ccttgccgcg cgcgccgtcg acaaaaccct tcgccaccag
     2641 cccggtgacc accttcatca ggtggctgcg cgagatgtcg aagcgttccg ccacttcctt
     2701 gatggtcacg cgtcgctcgg tgcaggtgcc gaggtacatg agcaggcgaa gcgcgtaatc
     2761 ggtgtgctgg gtgatgttca tcgcgaaggt gcgccgggcg tatggcgggc tgcatggtag
     2821 cagtaataga ttcatttctg ttgactcttt aaaagatgca tttaatatgc accttaaagg
     2881 cctcgaccga aggagctgcc atgaaacgcc acccccatct cgtccggctg tcgcgcgaac
     2941 accatgccgc gctgcggctc ggcaggcatc tgctcgccgg cggcgcggcg gccgagctgc
     3001 gcgcggaaca cgtcgcgctc gtcacccact ttgccgagga agagcgcgag ctggccccct
     3061 tgctcgaaac gggcggtcac ggcgcactcg ccgcgcgcct gcgcgccgag catgcccacc
     3121 tgaggtccct gttcgccacc gcagcggacg gcgtgcgcga ggccgcggcc gggcaggcgc
     3181 tgatcgacca tgtgcgcttc gaggagcgcg aactgtttcc ggcgatcgaa accctgttcg
     3241 aggaagtgct gccatgaccc atgtcgtcac cgaagcctgc atccgctgca aatacacgga
     3301 ttgcgtgagc atgtgcccgg tcgacgcctt tcgcgagggg ccgaacttcc tcgtgatcga
     3361 ccccgaggag tgcatcgact gcaccctgtg cgtcgccgag tgtccggtcg atgcgatcgt
     3421 gccggaagat gagttgagcg acgagcagcg cgaatacctc gccctcaacg cggcgctggc
     3481 gaaggactgg ccgcg
//
LOCUS       DAOQNN010000346         3489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18339_length_3489_cov_7.460105, whole genome shotgun sequence.
ACCESSION   DAOQNN010000346 DAOQNN010000000
VERSION     DAOQNN010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18339_length_3489_cov_7.460105"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            657..1373
                     /locus_tag="PKV97_14250"
     CDS             657..1373
                     /locus_tag="PKV97_14250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB07091.1"
                     /translation="MRPFLHVGGRDYLRCEACEATFLHPAQRPAADAECAEYRLHRND
                     PEDPGYRRFLARLVDPLRIRLAPAAQGLDYGCGPGPALAAMLREAGHPMRVWDPFFAP
                     DPDALARRYDFVTCTEVVEHFHAPAAEFARLDGLLRPGGWLGITTCLQTEDARFAAWH
                     YRRDPTHVVFYRAATFRVIAARFGWRCELPAKDVVLLHKPAPPAAAAPCLSPPNPDRE
                     SPCATKPSSTTSPTASPRSA"
     gene            1316..2098
                     /locus_tag="PKV97_14255"
     CDS             1316..2098
                     /locus_tag="PKV97_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNB07092.1"
                     /translation="MRYETLLYDLSDGIAEIRLNRPQRLNAVTQRLYDELNDALGRAE
                     VAREVRVVLLTGAGRAFCVGADLKEHKAGRSAFDRRQYLQGEQDVCRRLIDLKKPVVA
                     AVNGYALGAGAEIALASDFLLMAEGAQLGLPEISIGNFLGGGVTWLLPRLVGLAKARE
                     LVFLGERIGGEEAVRIGLANRVFADAGFLDEARAFARRIAAKAPFSMELAKRQLNLSA
                     ERTLDACLVAELEGMMFVGTTRDWQEGVDAFAEKRAPVFRGE"
     assembly_gap    2112..2211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2212..>3489
                     /locus_tag="PKV97_14260"
     CDS             <2212..>3489
                     /locus_tag="PKV97_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNB07093.1"
                     /translation="YPINPKVPEILGVPTCATLDDVPGPIDLALICTPAKTLPGLLEA
                     CGRKGVKGAVILASGFRESGPEGARLEQQVLDAARAGGVRIIGPNTSGMFNLHHKVNL
                     LDLRNARPGDIGFISQSGNMLLALVLEAEHNGHVGFSTYVGPGNQTDVGFNDYLRYLG
                     EDVHTRVATLYVEGFRDGQRFLEVAREITPVKPVVVYKSGATELGKKAASSHTGALAG
                     SYAMTVDLLRQVGVSVVHHSDEILPVAEGLALMQQARGRRIAVIADGGGQATITSDRL
                     SESGLELAELSAATRKRLAAILFPQAALANPVDVAGSSDADPEVLARCVEIVADDDNV
                     DAVFLVGMFGGYHIRFAESLLGGEMRAAEAMIELARASAKPLVVYSLYAPVKPPALRR
                     LHEAGLPVYASIEQAVKVLEALAERGLYLQDRH"
BASE COUNT          451 a         1196 c         1235 g          507 t
ORIGIN      
        1 gggcgggggt ggcgagcggc gtggtcgccg tggtcgcggc ggattgttgg gcgcgcgcga
       61 gcgcgggcag cgcgaggccg gccgcgagcg tgccggcgcg caggaagttg cggcggttca
      121 tgtgttctcc cgatgagcga agccgccccg gtgcgggatg ccgccggggc ttgatggttg
      181 gccgttgccg gcggccgtgt gcggggtgtg tccgcggcgc ggaaggccga tgcgaaggat
      241 cggcacgcgg gtccggcgcg cattgtcggc gattgtggag gccgcgtgaa ccgccgggga
      301 gatcgcgact tgcgtcgctc ctgcagagac cgtcgtgttg cgagccttgt cgtgggagcg
      361 acgcgagtcg cgatccagcg gttccgcggc gccgaaaatc gcgacttgcg tcgctcctac
      421 gagaacggta ctcaccccgc gggccttgtc gtgggagcga cgcgagtcgc gacccagtgg
      481 ctccgatgtg ccgcgcgcgg atccgcacga gcgtcagatg cagccccgtg ctccgcctgc
      541 ccccgggttc gggatctccg ccatgggcag ggccggcggg gctcgataca atcgcccgca
      601 tgaatcagcc ggctcccggg acggcgactt gctgcccggt ctgtcttggc gcatccgtgc
      661 gcccctttct gcacgtgggc gggcgcgact acctgcgctg cgaagcctgc gaggcgacct
      721 tcctgcaccc ggcccagcgg ccggcggcgg atgcggagtg cgccgaatac cgcctgcacc
      781 gcaacgaccc cgaggatccc ggctaccgcc gcttcctcgc gcgcctggtc gatccgctgc
      841 gcatccgcct cgcgcccgcg gcgcaggggc tggactacgg ctgcggtccg gggccggcgc
      901 tggccgcgat gctgcgcgag gccggccacc cgatgcgggt gtgggacccc ttcttcgccc
      961 ccgaccccga cgcgctcgcg cgccgctacg acttcgtcac ctgcaccgag gtggtcgagc
     1021 acttccacgc gcccgcggcc gagttcgccc gcctggacgg tctgctgcgc cccgggggct
     1081 ggctcggcat caccacctgc ctgcagaccg aggatgcgcg cttcgccgcc tggcactacc
     1141 ggcgcgatcc cacccacgtg gtgttctatc gcgcggccac cttccgggtg atcgccgccc
     1201 gcttcggctg gcgctgcgag ctgccggcca aggacgtggt gctgctgcac aagcccgcgc
     1261 cgccggcggc cgccgcgccg tgcctttctc cccccaaccc cgaccgggag tctccatgcg
     1321 ctacgaaacc ctcctctacg acctctccga cggcatcgcc gagatccgcc tgaaccgccc
     1381 gcagcgcctc aacgcggtga cgcagcggct ctacgacgag ttgaacgacg cgctcggccg
     1441 cgccgaggtt gcgcgcgagg tgcgcgtggt gctgctcacc ggcgcgggcc gcgccttctg
     1501 cgtcggcgcc gatctcaagg aacacaaggc tggacgcagc gccttcgacc gccgccaata
     1561 cctccagggc gagcaggacg tgtgccgccg cctgatcgac ctgaagaagc cggtggtcgc
     1621 cgcggtcaac ggctacgcac tcggcgccgg tgccgagatc gcgctcgcgt ccgacttcct
     1681 gctgatggcc gagggcgcgc agctcggcct gcccgagatc tcgatcggca acttcctcgg
     1741 cggcggcgtg acctggctgc tgccacgcct ggtcgggctg gcgaaggcgc gcgagctggt
     1801 cttcctcggc gagcgcatcg gtggcgagga ggcggtgcgc atcgggctgg cgaaccgggt
     1861 gttcgccgac gcgggcttcc tcgacgaggc gcgcgccttc gcgcgcagga tcgccgccaa
     1921 ggcgccgttc tcgatggagc tcgccaagcg ccagctcaat ctttcggccg agcgcacgct
     1981 cgacgcctgc ctcgtcgccg agctcgaggg catgatgttt gtcggcacca cgcgcgactg
     2041 gcaggagggc gtggatgcct tcgccgagaa gcgcgcgccg gtgttcaggg gggaatgaca
     2101 tgaacgagaa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntctacccga
     2221 tcaaccccaa ggtgcccgag atcctcggcg tgccgacctg cgccacgctc gacgacgtgc
     2281 ccgggccgat cgacctggcg ctgatctgca ccccggcgaa gaccctgccc gggctgctcg
     2341 aggcgtgcgg gcgcaagggc gtgaagggcg cggtgatcct cgccagcggc tttcgcgaga
     2401 gcgggccgga gggcgccagg ctcgagcagc aggtgctgga cgcggcgcgt gcgggcggcg
     2461 tgcgcatcat cggcccgaac acctcgggca tgttcaacct gcatcacaag gtgaacctgc
     2521 tcgacctgcg caacgccagg cccggcgaca tcggcttcat ctcgcagtcg ggcaacatgc
     2581 tgctggcgct ggtgctggag gccgagcaca acggccacgt gggtttctcg acctacgtcg
     2641 gcccgggtaa ccagaccgac gtcggcttca acgactacct gcgctacctc ggcgaggacg
     2701 tgcacacccg ggtggcgacg ctgtatgtgg agggcttccg cgacggccag cgctttctcg
     2761 aggtggcgcg cgagatcacc ccggtgaagc cggtggtggt gtacaagtcg ggcgccaccg
     2821 agctcggcaa gaaggcggcg agctcgcaca ccggcgcgct cgccggcagc tacgcgatga
     2881 cggtggacct gctgcgccag gtgggggtga gcgtggtgca tcactccgac gagatcctgc
     2941 cggtggccga gggcctggcg ctgatgcagc aggcgcgtgg gcggcggatt gcggtgatcg
     3001 ccgacggtgg cggccaggct acgatcacct ccgaccgctt gtcggagtcc ggcctggaac
     3061 tggccgagct gtctgcggcg acgcgcaagc gcctggcggc gatcctgttc ccgcaggccg
     3121 cgttggcgaa tccggtggat gtggccggca gctcggacgc cgaccccgag gtgctggcgc
     3181 gctgcgtgga gatcgtcgcc gacgacgaca acgtggacgc ggtcttcctc gtcggcatgt
     3241 tcggcggcta ccacatccgc ttcgccgagt ccctgctcgg cggcgagatg cgcgcggccg
     3301 aggccatgat cgagctcgcg cgcgccagcg caaagccgct ggtggtctac agcctctacg
     3361 ccccggtcaa gccgcctgcg ctacgccgcc tgcacgaggc cgggctgccg gtgtacgcct
     3421 ccatcgagca ggcggtgaag gtgctggagg cgctcgccga gcgcgggctc tacctgcagg
     3481 accgccacg
//
LOCUS       DAOQNN010000347         3477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18432_length_3477_cov_9.325248, whole genome shotgun sequence.
ACCESSION   DAOQNN010000347 DAOQNN010000000
VERSION     DAOQNN010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18432_length_3477_cov_9.325248"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PKV97_14265"
     CDS             <1..795
                     /locus_tag="PKV97_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNB07094.1"
                     /translation="AATVPALFAAGLFPGIVAALCLLVPAIVISRKYGFGRDYKAARP
                     PLGKSFIEAIWGLLAPVIILGGLRTGIFTPTEAAVVAVAYGIFVGMVVYRSISFRGLF
                     RVLVEAGELSAVVLMIIGIASVFAWAGNTLGIFDAAAKALVGLHSSEWLMLLSINLLL
                     LAAGMFLDAISIFLILLPLLVPIAVAMGWDLVWFGVMMTINLAIGQFTPPMAISLMIT
                     SRIAGIGMEETFKWVIWLVLAMGAGLTLMIVFPQLALWLPGKLGYL"
     gene            complement(871..1272)
                     /locus_tag="PKV97_14270"
     CDS             complement(871..1272)
                     /locus_tag="PKV97_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF995 domain-containing protein"
                     /protein_id="HNB07095.1"
                     /translation="MNRLLACAAAALLLSACASTEKELADKGMKPLDAAKVRTLLSGN
                     TATGTTASGSGFMIYNAPDGTTRGKSGNSSDTGKWDVTADGQHCNHWTSWRGGERRCA
                     RLYEADGEIRYFDGGNYAGRFKIEQGNPYKL"
     gene            complement(1401..2024)
                     /locus_tag="PKV97_14275"
     CDS             complement(1401..2024)
                     /locus_tag="PKV97_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB07096.1"
                     /translation="MQITDPTPHTTEASRATARRQQILAAAAQCFREHGFHGASIARI
                     SKIAGMSAGHIYHYFENKEAIIAAIVAQDLETLLTLTAELRAACNVRHALIERAAEGV
                     LDQLDPDSAALKVEIVAEAARNSHVAQIVREADATCRRELMVTIRAIRSAAGHDDTPE
                     ATADMVEVLAALFEGLLLRTIRNPELDRKHVAQRIQLAINDLLDQPG"
     gene            2223..3425
                     /locus_tag="PKV97_14280"
     CDS             2223..3425
                     /locus_tag="PKV97_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB07097.1"
                     /translation="MKTSSQPLFRSAAAVALSALVLAACSGGKEQQGGAPGAGAPPQA
                     PAVTVMTVASGSVPMVTELPGRTTPYLIAELRPQVTGILTQRAFDEGSEVKAGQVLYR
                     IDDAPYQAAHDSAKAALARAEANAQVARIKAERHAGLVKIDAVSKQANDDTQAALKQA
                     QAEVAAAKAALDKARIDLDYTRLKSPISGRIGRSAVTPGALVTANQVQALATVQQLDP
                     IYVDLTQSSADMLRMRAEIAAGRLQAGAKGEVPVKLILEDGSEYAVEGRLALSEVTVD
                     EGTGSVTLRARFPNADGVLLPGMYVRARLPQGTRSEAILVPHKALSRDPLGNALVMVV
                     NAESKVEARPVKVAQSLASDWVVTGGLAAGERIIVEGLQKARPGAPVQAQEAGATPAA
                     ATPAAKAS"
BASE COUNT          499 a         1134 c         1242 g          602 t
ORIGIN      
        1 gcggcgaccg tgccggcgct gttcgccgcc ggcctgttcc ccggcatcgt cgccgcactg
       61 tgcctgctcg tgccggcgat cgtgatcagc cgcaagtacg gcttcggccg cgactacaag
      121 gccgcgcgcc cgccgctcgg caagagcttc atcgaggcga tctggggcct gctggcgccg
      181 gtgatcatcc tcggcggcct gcgcaccggc atcttcacgc ccaccgaggc ggcggtggtg
      241 gcggtggcct acggcatctt cgtcggcatg gtggtgtatc gcagcatcag cttcaggggc
      301 ctgttccgcg tgctggtcga ggccggcgag ctgtcggcgg tggtgctgat gatcatcggc
      361 atcgcctcgg tattcgcctg ggcgggcaac acgctgggca tcttcgacgc cgcggcgaag
      421 gcgctggtcg gcctgcactc cagtgaatgg ctgatgctgc tgtcgatcaa cctgctgctg
      481 ctcgcggcag ggatgttcct cgacgcgatc tcgatcttcc tgatcctgct gccgctgctg
      541 gtgccgatcg cggtggcgat gggctgggac ctggtgtggt tcggggtgat gatgacgatc
      601 aacctcgcca tcggccagtt caccccgccg atggcgatct ccctgatgat cacctcgcgc
      661 atcgccggga tcggcatgga ggagaccttc aagtgggtga tctggctggt gctggcgatg
      721 ggtgccgggc tgacgctgat gatcgtgttc ccgcagctcg cgctgtggct gccggggaag
      781 ctcggctacc tgtaaggccc ggattcagtg gggtcggact cgatcggtcg ccgaccggga
      841 cgatcgatcg agtctgcccc ccattgattc tcagagcttg tagggattgc cctgctcgat
      901 cttgaagcgg ccagcgtagt tgccgccgtc gaaatagcgg atttcgccgt ccgcctcgta
      961 cagccgcgcg cagcgccgct cgccgccgcg ccaggaggtc caatggttgc agtgctggcc
     1021 gtcggcggtg acgtcccact tgccggtgtc gctgctgttg ccgctcttgc cgcgcgtggt
     1081 gccgtcgggg gcgttgtaga tcatgaagcc gctgccggac gcggtggtgc cggttgcagt
     1141 gttgccggac agcagggtgc ggaccttcgc cgcgtcgagc ggcttcatgc ccttgtcggc
     1201 aagttctttc tcggtggatg cgcaggcact cagcagcagt gccgccgccg cgcaggcgag
     1261 cagtctgttc atttgggtct ccgtcgggac gttgttgtgg tcctttgccg ttgcgcggcg
     1321 cgagagcagt gcgccgctat gactggtcta ggacttggtc atgcgttcgt caagccgagg
     1381 ggatgcctgg tgccgaaagc tcagcccggc tggtcgagca ggtcgttgat ggcgagctgg
     1441 atgcgctgcg ccacgtgctt gcggtcgagc tcggggttgc ggatcgtgcg cagcagcagg
     1501 ccttcgaaca gggctgcgag cacttcgacc atgtcggcag ttgcttccgg cgtgtcgtcg
     1561 tggccggcgg cgctgcggat ggcgcggatg gtgaccatca gttcgcgccg acaggtggcg
     1621 tcggcctcgc ggacgatctg cgccacgtgt gagttgcgcg cggcctcggc gacgatctcc
     1681 accttcagcg ctgcgctgtc ggggtcgagc tgatccagca cgccttcggc ggcgcgttcg
     1741 atcaaggcat ggcgtacgtt gcaggcggcg cgcagctcgg cggtcagggt cagcagggtt
     1801 tcaagatcct gcgccacgat cgcggcgatg atcgcttcct tgttctcgaa gtagtggtag
     1861 atatggccag cactcatgcc ggcgatcttg gagatccgtg cgatgctggc gccatgaaag
     1921 ccgtgctcgc ggaagcactg cgcggcggcg gcgaggatct gctggcgacg ggcggtggcg
     1981 cgcgaggctt cggtagtgtg gggcgtgggg tccgtgatct gcatgctgct ccggtcaggc
     2041 cttcactccc gccggcgatg aaccaccggg gcgggacgat gtccttaaga atcttgcgat
     2101 gcagcaaacc ataacataga atgagcgttc gatctagaat aaccattcta atgagcggga
     2161 tgtcggccag cgatcaacag tcgcacgccg gtgtcgtcgt ttctcgcgtt cacgaggctc
     2221 caatgaagac aagctcccaa ccccttttca ggtccgcggc tgcggtggcg ctgagcgccc
     2281 tggtcctggc cgcctgcagc ggaggcaagg aacaacaggg cggcgcgccg ggcgccgggg
     2341 cgccgccgca ggcgccggcg gtgaccgtga tgacggtcgc cagcggcagc gtgccgatgg
     2401 tgaccgagct gcccggccgc accacgccct acctgatcgc cgagctgcgc ccgcaagtca
     2461 ccggcatcct cacccagcgc gccttcgacg agggcagcga ggtcaaggcc gggcaggtgc
     2521 tctaccgcat cgacgacgcc ccctaccagg ctgcccacga cagcgccaag gcggcgctcg
     2581 cccgtgccga ggccaatgcc caggtcgcgc gcatcaaggc cgaacgccac gccgggctgg
     2641 tcaagatcga cgctgtcagc aagcaggcca acgacgacac ccaggccgcg ctgaagcagg
     2701 cccaggccga ggtcgccgcg gcgaaggcgg cgctcgacaa ggcgcgcatc gacctcgact
     2761 acacccgcct caagtcgccg atctccgggc gcatcggacg ctcggcggtg acgccgggcg
     2821 cgctggtcac cgccaaccag gtgcaggcgc ttgccaccgt gcagcagctc gacccgatct
     2881 acgtcgacct cacccagtcg agcgccgaca tgctgcgcat gcgcgcggag atcgccgccg
     2941 gccgcctgca ggcgggcgcg aagggcgagg tgccggtgaa gctgatcctc gaggacggca
     3001 gcgagtacgc cgtcgagggt cgcctggcgc tctccgaggt cacggtggac gaaggcaccg
     3061 gcagcgtgac cctgcgcgcg cgcttcccca acgcggacgg cgtgctgctg ccgggcatgt
     3121 acgtgcgtgc gcgcctgccg cagggcacgc gcagcgaggc catcctggtg ccgcacaagg
     3181 cgctgtcgcg cgacccgctc ggcaacgcgc tggtgatggt ggtgaatgcc gagagcaagg
     3241 tcgaggcgcg tccggtgaag gtggcgcaat cgctcgcctc ggactgggtg gtgaccggcg
     3301 gcctcgcggc cggcgaacgc atcatcgtcg aaggcctgca gaaggcccgc ccgggtgcgc
     3361 cggtgcaggc gcaggaggcc ggcgcgacgc ccgccgccgc gacgcccgcc gccaaggcca
     3421 gctgaggtag ccgaccatgg cccgtttctt catcgaccgt cccatcttcg cgtgggt
//
LOCUS       DAOQNN010000348         3477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18433_length_3477_cov_7.507306, whole genome shotgun sequence.
ACCESSION   DAOQNN010000348 DAOQNN010000000
VERSION     DAOQNN010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18433_length_3477_cov_7.507306"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1200)
                     /gene="pheA"
                     /locus_tag="PKV97_14285"
     CDS             complement(133..1200)
                     /gene="pheA"
                     /locus_tag="PKV97_14285"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNB07098.1"
                     /translation="MSDELLKLRNEIDRIDEEILARLAERARCAQRVGEIKRGVMYYR
                     PEREAQVLRRLAELNPGPLSSDAVKTIFREVMSACLGLEQPLRVAYLGPAGTFSESAS
                     RKHFGSAPNFLAMAAIDDVFRAVEAGNADYGVVPVENSTEGAVGGTLDLLLANPLKVC
                     GEVRLRIHQQLMSRAEGIGAARRIYSHAQSLAQCHEWLNRNLPHLPRIPVASNAEAAR
                     MASEDPESCAIAGDAAAQLYGLNILAPNIEDDPNNTTRFLVIADHDAGPSGKDRTSLV
                     FSAPNRPGAIHSLLEPMARHGVDMTKLQSRPARSGLWEYVFYADINGHREDPEVAAAL
                     RELDERAAFVKIIGSYPVAAI"
     gene            complement(1280..2377)
                     /gene="serC"
                     /locus_tag="PKV97_14290"
     CDS             complement(1280..2377)
                     /gene="serC"
                     /locus_tag="PKV97_14290"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNB07099.1"
                     /translation="MSRVWNFSAGPAVLPEEVLRQAADEMLDWHGSGMGVMEMSHRGK
                     EFTAIAAQAEADLRELLAVPANYRILFMQGGAIAENAIIPMNLMGDRRVADYVVTGSW
                     SQKSQKEARKYAEVNIAASSEAGGFTTVPAMADWRLSADPAYVFTCTNETIGGLEYPF
                     EPDLAQIGRGEVPVVADMSSHILSRVIDVSKYGLIFGGAQKNIGPAGLTIVIVREDLL
                     GRAMPHCPTAFDFKTVADNGSMYNTPPTYGIYIAGLVFQWLKRQGGVAAIEAQNVAKA
                     KLLYDFLDASDFYENRIDPACRSRMNVPFFLKDETLNDAFLAEAKAAGLVQLKGHKSV
                     GGMRASIYNAMPLAGVQALVDFMRDFAARRG"
     gene            complement(2377..>3477)
                     /locus_tag="PKV97_14295"
     CDS             complement(2377..>3477)
                     /locus_tag="PKV97_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNB07100.1"
                     /translation="RSELVMNTAEINIEDLITPEDMVVTLSHTGYFKRQPLADYRAQR
                     RGGRGKQATSMKDEDFIDHLFVANTHDTVLCFSNRGRCYWLKVYEVPEGTRNSRGKPI
                     VNLFPLVEGEKITAVLPIKEFDEEHFVFMATSEGTVKKTALTAFSNPRKAGIIAVNLD
                     DGDHLIGVAITDGQCDVMLFSDAGKAVRFAETDVRPMGRDARGVRGMTLEDGQAVIAM
                     LVAKDESQSVLTATENGYGKRTSIVEYTRHGRGTKGMIAIQTSDRNGRLVGAVLVEPS
                     DEVMLISTGGVLIRTSVESIREMGRSTQGVTLINLDEGTFLASIEKVAESESDESMRA
                     SGEGAEGGEAEGGAQAAPGEGEAPAADEEGEA"
BASE COUNT          475 a         1189 c         1210 g          603 t
ORIGIN      
        1 agatcggctt gcccggctgg taggccatga tcgcgcggat gtagtcgggg gcaagactgg
       61 caatgctcat gggtcgtctc ctgctgcggc ccgcactccc cgcgcgggcc tggtgtgcaa
      121 tcggggccgc gctcagatcg ccgcgaccgg ataggaaccg atgatcttca cgaaggcggc
      181 gcgctcgtcg agctcgcgca gcgcggccgc cacctcgggg tcttcgcggt ggccgttgat
      241 gtcggcgtag aacacgtact cccacagccc ggagcgcgcc gggcgcgact gcagcttggt
      301 catgtccacg ccgtggcggg ccatcggctc gagcaggctg tggatcgccc ccggccggtt
      361 gggcgcggag aacaccagcg aggtgcggtc cttgcccgag ggcccggcgt cgtggtcggc
      421 gatgaccagg aagcgcgtcg tgttgttggg atcgtcctcg atgttgggcg cgaggatgtt
      481 gagcccgtag agctgcgccg cggcgtcgcc ggcgatggcg caggactcgg gatcctcgga
      541 ggccatgcgc gcggcctcgg cgttgctcgc caccgggatg cgcggcaggt gcgggaggtt
      601 gcggttgagc cactcgtggc actgcgccag cgactgcgcg tgcgagtaga tgcggcgggc
      661 ggcgccgatg ccctcggcgc gcgacatcag ctgctggtgg atgcgcaggc gcacctcgcc
      721 gcagaccttg agcgggttgg ccagcagcag atcgagcgtg ccgccgaccg cgccctcggt
      781 ggagttctcc accggcacca cgccgtagtc ggcattgccg gcttccaccg cgcggaagac
      841 gtcgtcgatc gccgccatcg ccaggaagtt gggcgcggaa ccgaagtgct tgcggctggc
      901 gctctcggag aaggtgccgg cggggccgag gtaggccacg cgcagcggct gctccagccc
      961 gaggcaggcc gacatcacct cgcggaagat ggtcttcacc gcatcggaag acagcgggcc
     1021 ggggttgagc tcggccagcc ggcgcagcac ctgcgcctcg cgctcggggc ggtaatacat
     1081 caccccgcgc ttgatctcgc ccacgcgctg cgcgcagcgc gcgcgctcgg cgaggcgggc
     1141 gaggatttcc tcgtcgatgc ggtcgatctc gttgcgcagt ttcagcagtt cgtcgctcat
     1201 gctcggcccg tggcccctcc ggcttgcgtc ctgatgttgt cccgtgtttc cgtcccgcct
     1261 tgccctgccc cgcgcggcct cagccgcggc gggcggcgaa gtcgcgcatg aagtcgacca
     1321 gcgcctgcac gcccgcgagc ggcatcgcgt tgtagatcga cgcgcgcatg ccgccgaccg
     1381 acttgtgacc cttcagctgc accagcccgg cggccttggc ctcggcgagg aaggcgtcgt
     1441 tcaaggtctc gtccttgagg aagaagggca cgttcatgcg cgaacggcag gccgggtcga
     1501 tgcgattctc gtagaaatcg ctggcgtcga ggaagtcgta cagcagcttc gccttggcga
     1561 cgttctgcgc ctcgatcgcc gccaccccgc cctggcgctt gagccactgg aagaccagcc
     1621 cggcgatgta gatgccgtag gtgggcggcg tgttgtacat cgagccgttg tcggccacgg
     1681 tcttgaagtc gaaggcggtg gggcagtgcg gcatcgcgcg cccgagcagg tcctcgcgca
     1741 cgatcacgat ggtgagaccc gccgggccga tgttcttctg cgcgccgccg aagatgaggc
     1801 cgtacttcga gacgtcgatc acccgcgaca ggatgtgcga ggacatgtcg gccaccaccg
     1861 gcacctcgcc gcggccgatc tgcgcgaggt cgggctcgaa ggggtactcg aggccgccga
     1921 tggtctcgtt ggtgcaggtg aacacgtagg ccgggtcggc cgacagcctc cagtccgcca
     1981 tcgccggcac ggtggtgaag ccgcccgcct cggacgaggc ggcgatgttc acctcggcgt
     2041 acttgcgcgc ttccttctgc gacttctgcg accacgagcc ggtgaccacg tagtcggcca
     2101 cgcgtctgtc gcccatcagg ttcatcggga tgatcgcgtt ctcggcgatc gccccgccct
     2161 gcatgaagag gatgcggtag ttggccggca ccgcgagcag ctcgcgcagg tcggcctcgg
     2221 cctgggcggc gatggcggtg aactccttgc cgcgatggct catctccatc acgcccatgc
     2281 cgctgccgtg ccagtcgagc atctcgtcgg cggcctggcg cagcacctct tcgggcagca
     2341 ccgcgggacc ggcgctgaag ttccacacgc gactcatcag gcctctcctt cctcgtccgc
     2401 agcgggggcc tcgccctcgc ccggcgcggc ttgtgcgccg ccttcggcct cgccgccctc
     2461 ggcgccctcg ccgctggcac gcatgctctc gtccgactcg gactcggcca ccttctcgat
     2521 gctggcgagg aaggtgccct cgtccaggtt gatcagggtc acgccctggg tcgagcggcc
     2581 catctcgcgg atgctctcga cgctggtgcg gatcagcacg ccgccggtgg agatcagcat
     2641 cacctcgtcg ctcggctcga ccagcaccgc gcccaccagc ctgccgttgc ggtcgctggt
     2701 ctggatcgcg atcatgccct tggtgccgcg gccgtggcgg gtgtactcca cgatcgaggt
     2761 gcgcttgccg tagccgtttt cggtcgcggt cagcaccgac tgcgactcgt ccttggccac
     2821 cagcatcgcg atcaccgcct gaccgtcctc cagcgtcatg ccgcgcacgc cgcgcgcgtc
     2881 gcgccccatc gggcgcacgt cggtctcggc gaagcgcacc gccttgccgg catccgagaa
     2941 cagcatcacg tcgcactggc cgtcggtgat tgccaccccg atcaggtggt cgccgtcgtc
     3001 caggttgacc gcgatgatgc cggccttgcg cggattggag aacgcggtga gcgcggtctt
     3061 cttgaccgtg ccttccgagg tcgccatgaa cacgaagtgc tcctcgtcga actccttgat
     3121 cggcagcacc gcggtgatct tctcgccttc caccagcggg aagaggttca cgatcggctt
     3181 gccgcgcgag ttgcgcgtgc cctccgggac ctcgtacacc ttgagccagt agcagcgccc
     3241 gcggttggag aagcacagca ccgtgtcgtg agtgttggcg acgaagaggt ggtcgatgaa
     3301 gtcctcgtcc ttcatcgagg tcgcctgctt gccgcggcca ccgcggcgct gggcgcggta
     3361 gtcggcgagc ggctggcgct tgaagtagcc ggtgtgcgac agggtcacga ccatgtcttc
     3421 cggcgtgatc aggtcctcga tgttgatctc ggcggtgttc atcaccagct cggagcg
//
LOCUS       DAOQNN010000349         3473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18466_length_3473_cov_9.038327, whole genome shotgun sequence.
ACCESSION   DAOQNN010000349 DAOQNN010000000
VERSION     DAOQNN010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18466_length_3473_cov_9.038327"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /gene="nadD"
                     /locus_tag="PKV97_14300"
     CDS             <1..651
                     /gene="nadD"
                     /locus_tag="PKV97_14300"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNB07101.1"
                     /translation="PLGVLGGTFDPIHTGHLRLAEEAREALGLDGVRFIPAGQPPHRG
                     EPGSTAEDRLAMARLATAGNPGFSVDDGEVRAQQKSYTVPTLERLRAEIGPQQPLVLI
                     LGADAFAGLPTWHRWTDLFALAHIAVANRPGYAPHGRRWPATLSPALDAACAGRHTAD
                     PADLRRAPAGRVVPFDMTPLAISASLIRDLVRDGHSARYLLPESVLDYIAAHHLYR"
     gene            682..1029
                     /gene="rsfS"
                     /locus_tag="PKV97_14305"
     CDS             682..1029
                     /gene="rsfS"
                     /locus_tag="PKV97_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNB07102.1"
                     /translation="MDTPTLEKIVVAALEDIKARDIEVIDTSKHTPLFDRIVVASAES
                     GRQTRALAQNVHDKVKEAGGEIIGTEGQETGEWVLVDLGNIVVHVMQPAVRAHYKLEE
                     LWNTPPSSRRNAA"
     gene            1127..1597
                     /gene="rlmH"
                     /locus_tag="PKV97_14310"
     CDS             1127..1597
                     /gene="rlmH"
                     /locus_tag="PKV97_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNB07103.1"
                     /translation="MKLIIVAVGHRMPAWVQAGFDEFARRMPRELQLQLVEVKAEPRS
                     GGKTVEAMMAAEAARIEATLPPRCRRVILDERGADLSTLALARRLESWQGAGDDVALI
                     VGGPDGLAPAFKAGAHEAIRLSSLTLPHALVRPLLAEALYRAWTVLKNHPYHRE"
     gene            1804..2424
                     /locus_tag="PKV97_14315"
     CDS             1804..2424
                     /locus_tag="PKV97_14315"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNB07104.1"
                     /translation="MTLTPRIYLASTSPRRRDLLRQIGVNFDVLTFRAGERGEDADVD
                     ETPLPDEAVERYVERLALAKAQAGMRRVLWRKLLPHPVLAADTTLEVDGEIVGKPREA
                     AEAHAILERLSGRRHRVLTAVAMSDGERTRSRLSVSEVAFRTLSEHDIRRYIATGEPF
                     DKAGAYGIQGHAAIFIEEIRGSYSGIMGLPLFETAALLEIFGHPVL"
     gene            2438..>3473
                     /locus_tag="PKV97_14320"
     CDS             2438..>3473
                     /locus_tag="PKV97_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNB07105.1"
                     /translation="MSIEFLINFTPQETRVAIVEQGVVQELHVERTASRGIVGNLYLG
                     KVVRVLPGMQSAFIDIGLERTAFLHVADIWSDRQNGEGARPIEKILTEGQSLMVQVLK
                     DPIGTKGARLSTQVSLAGRLLVYLPQEKHIGISQRIEDEAGREALRSRLVALVPEDEP
                     GGFIVRTMAESATDAELAADIAYLRKLWREISGSTVGLFPPKLLHEDLGLGQRTLRDL
                     VTDETSRIRVDSRENFQKLTAFAAEYTPQVLPLLEHYSGDRPLFELYSVEEEIQKALA
                     RRVDLKSGGYLIIDQTEAMTTVDVNTGGFVGARNFDDTIFKTNLEASQAIARQLRLRN
                     LGGIIIIDFID"
BASE COUNT          552 a         1366 c         1098 g          457 t
ORIGIN      
        1 ccgctcggcg tgctcggcgg caccttcgac cccatccaca ccggccacct gcgcctcgcc
       61 gaagaagcgc gcgaggcgct cggcctcgac ggcgtgcgct tcatccccgc cggtcagccg
      121 ccgcaccgcg gcgagcccgg cagcaccgcc gaggaccgcc tcgccatggc acggctcgcc
      181 accgccggca accccggctt cagcgtggac gacggcgagg tgcgcgcgca gcagaagagc
      241 tacaccgtgc ccaccctcga gcgcctgcgc gccgagatcg gcccgcagca gccgctggtg
      301 ctgatcctcg gcgccgacgc cttcgccggg ctgccgacct ggcaccgctg gaccgatctg
      361 ttcgcgctcg cccacatcgc ggtcgccaac cgccccggct acgcgcccca cggccggcgc
      421 tggcccgcca ccctgtcgcc cgcgctcgac gccgcctgcg cgggccgcca caccgccgac
      481 cccgcggatc tgcgccgcgc accggcgggc cgcgtcgtcc ccttcgacat gaccccgctg
      541 gcgatctccg cctcgctgat ccgcgatctg gtgcgcgacg gccacagtgc ccgctatttg
      601 ctgccggaat cggttctcga ctatattgcc gcgcaccatc tctaccgctg agcgccccgc
      661 gccggcccac acaggatccg aatggacacc cccaccctcg aaaagatcgt cgtcgccgcc
      721 ctcgaagaca tcaaggcacg cgacatcgaa gtcatcgaca cctcgaagca cacccccctc
      781 ttcgaccgca tcgtcgtcgc cagcgccgaa tccggccgtc agacccgggc gctcgcgcag
      841 aacgtgcacg acaaggtcaa ggaagccggc ggcgagatca tcggcaccga aggccaggag
      901 accggcgagt gggtgctggt cgacctcggc aacatcgtgg tgcacgtgat gcagcccgcg
      961 gtgcgcgcgc actacaagct cgaggaactc tggaacaccc cgccgtccag ccggcgcaac
     1021 gccgcctgac gagtagcgcc ccgcgcgagc ggccgcctgc gtacggcgcg ggtggcggtc
     1081 gcgtttttcg acgcatgctc gtccacgcac gcgcgcaggt tcaaccatga aactgatcat
     1141 cgtcgccgtc ggccaccgca tgcccgcctg ggtgcaggcc ggcttcgacg agttcgcccg
     1201 ccgcatgccg cgcgagctgc agttgcagct cgtcgaggtc aaggccgagc cgcgcagcgg
     1261 cggcaagacg gtggaggcga tgatggcggc cgaggccgcg cgcatcgagg ccaccctgcc
     1321 gccgcgctgc cgccgcgtga tcctcgacga gcgcggcgcc gacctcagca cgctggcgct
     1381 cgcgcgccgg ctggagtcct ggcagggcgc aggcgacgac gtcgcgctca tcgtcggcgg
     1441 ccccgacggc ctcgccccag cgttcaaggc gggcgcccac gaggccatcc gcctctccag
     1501 cctcaccctc ccccacgcct tggtgcgccc cctgctggcc gaggcgctct accgcgcgtg
     1561 gacggtactc aagaaccacc cctaccaccg cgagtagcgc ctgcggcatc ggccgatcgc
     1621 gacttgcgtc gctcccacaa gaaccaccgc gtctccgaag gcccccgtgg gagcgacgcg
     1681 agtcgcgatt gcggcggtga tgccggcacg gcggcgcggg cgggcggtga aacccgttag
     1741 aatcgcgcca cccgagcccg accggcgccg accggcgtcg cggatcctgc ggaacgccat
     1801 gcaatgaccc tcaccccgcg catctacctt gcctccacga gcccccgtcg ccgcgacctt
     1861 ctgcggcaga tcggcgtcaa tttcgacgtg ctcaccttcc gcgccggcga gcgcggcgag
     1921 gatgccgatg tcgacgagac ccccctgccc gacgaggcgg tcgaacgcta cgtcgaacgc
     1981 ctcgcgctcg ccaaggccca ggccgggatg cggcgggtgc tgtggcgcaa gctgctgccg
     2041 catccggtgc tcgccgccga caccacgctg gaggtcgacg gcgagatcgt cggcaagccg
     2101 cgcgaggccg ccgaggcgca cgccatcctg gagcgcctgt ccggccgccg ccaccgcgtg
     2161 ctgaccgcgg tcgcgatgag cgacggcgag cgcacgcgca gccggctgtc ggtcagcgag
     2221 gtcgccttcc gcacgctgag cgagcacgac atccgccgct acatcgccac cggcgaaccc
     2281 ttcgacaagg ccggcgccta cggcatccag ggtcacgccg cgatcttcat cgaggagatc
     2341 cgcggcagct actcggggat catgggcctg ccgctgttcg agaccgccgc cctgctcgag
     2401 atcttcggcc accccgtcct gtgagcacac cccgtccatg agcatcgaat tcctcatcaa
     2461 cttcaccccg caggaaaccc gcgtcgcgat cgtcgagcag ggcgtggtgc aggagctgca
     2521 cgtcgagcgc accgccagcc gcggcatcgt cggcaacctc tacctcggca aggtcgtccg
     2581 ggtgctgccc ggcatgcagt cggccttcat cgacatcggc ctcgagcgca ccgccttcct
     2641 gcacgtggcc gacatctgga gcgaccgcca gaacggcgag ggtgcgcgtc cgatcgagaa
     2701 gatcctcacc gaaggccaga gcctgatggt gcaggtgctc aaggacccga tcggcaccaa
     2761 gggcgcacgc ctgtccaccc aggtcagcct cgccggccgc ctgctcgtct acctgccgca
     2821 ggaaaagcac atcggcatct cccagcgcat cgaggacgag gccgggcgcg aggcgctgcg
     2881 cagccggctg gtcgcgctgg tgcccgaaga cgagccgggc ggcttcatcg tgcgcaccat
     2941 ggccgaatcc gccaccgacg ccgagctcgc cgccgacatc gcctacctgc gcaagctgtg
     3001 gcgcgagatc tccggctcga ccgtgggcct gttcccgccg aagctgctgc acgaggacct
     3061 cgggctgggc cagcgcacgc tgcgcgacct cgtcaccgac gagacctcac gcatccgcgt
     3121 cgactcgcgg gagaacttcc agaagctcac cgccttcgcc gccgaataca cgccgcaggt
     3181 gctgccgctg ctcgagcact acagcggcga tcgcccgctc ttcgagctct acagcgtgga
     3241 ggaggagatc cagaaggcgc tcgcccgccg cgtcgatctc aaatccggcg gctacctgat
     3301 catcgaccag accgaggcga tgaccaccgt cgacgtcaac accggcggct tcgtcggcgc
     3361 gcgcaacttc gacgacacca tcttcaagac caacctcgag gccagccagg ccatcgcccg
     3421 ccagctgcgc ctgcgcaacc tcggcggcat catcatcatc gacttcatcg aca
//
LOCUS       DAOQNN010000350         3453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18637_length_3453_cov_12.043849, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000350 DAOQNN010000000
VERSION     DAOQNN010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18637_length_3453_cov_12.043849"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1003)
                     /locus_tag="PKV97_14325"
     CDS             complement(161..1003)
                     /locus_tag="PKV97_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB07106.1"
                     /translation="MADRLAGLLRHYSLSARVFHSGAFCGQHHYAAPHGYIHLVRRGP
                     ITARSPVHEDLLVTEPSLLFYPRVASHRFVAAPGDTAELLCAEVDLGASTGNPLAMAL
                     PSMLLIPLADLPGLGPTLELLFAEAERDQCGRQAAIDRLCELLLIQLLRYLMDGRLGA
                     TGLLAGLADPKLARAITAMHDAPQTAWSLEALAAKAGMSRARFAAAFKDAVGVTPGDY
                     LADWRMNVSCTLLKQGRPVAVVADRVGYGSPNALARAFRVRMGCAPRDWLAQQRGDAA
                     LSGS"
     gene            1121..1654
                     /locus_tag="PKV97_14330"
     CDS             1121..1654
                     /locus_tag="PKV97_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNB07107.1"
                     /translation="MTQIAPLTIDTADAATAATLKAVKAKLGMVPNLFATLAHAPAAL
                     NGYLGLSETLGTGRLNASQREIVALAAGQANRCQYCLSAHTLIGKGAGLSAEAIAAAR
                     TGQAANALDDAIAGFARALVEQRGVVSADAMANYRRAGLDDGLILEVIANVALNTLTN
                     YTNHIADTTVDFPVVAV"
     gene            1682..2230
                     /locus_tag="PKV97_14335"
     CDS             1682..2230
                     /locus_tag="PKV97_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HNB07108.1"
                     /translation="MKIALTGALLASALVLPLAVTAGDFSPYVDSQGGISRPTDFRTN
                     FVHLGSYAVLDEKSASRGLHDVYTEKASAEHYRKTGKFLDGATLVKEIRKLETSAMTT
                     GNPVVWGSDAAVWFVMVKDAKGRFASNPLWGDGWGWALFKADAPAKNVAVSYEADCMG
                     CHVPAAKTDRVFIQGYPTLTQH"
     gene            2304..2579
                     /locus_tag="PKV97_14340"
                     /pseudo
     CDS             2304..2579
                     /locus_tag="PKV97_14340"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
     gene            2641..2904
                     /locus_tag="PKV97_14345"
     CDS             2641..2904
                     /locus_tag="PKV97_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07109.1"
                     /translation="MQRFERLSLVIVLGSYAMDYHLGTGKTPLTRVVEAWREHWPQAF
                     PLPHPSPRNNRWLVRNPWFQQDVLPALQARVQAVLTANPKETP"
     gene            complement(3088..>3453)
                     /locus_tag="PKV97_14350"
     CDS             complement(3088..>3453)
                     /locus_tag="PKV97_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HNB07110.1"
                     /translation="QHNMTARQASNQLPITDTPLILNDKTTRLSRLTSEWFASDGRQP
                     TPRIQLNYNDAIKSLVAAGYGATLLPHEASTPLPDTRIVMRPLQPLLWRQLGIAHRGG
                     DVERPTQHVLDVLWGLSAG"
BASE COUNT          668 a         1218 c         1008 g          559 t
ORIGIN      
        1 gacaagccac gttgatcggc tgacggaatt ttcggcggtt ccggcttaga acccccactt
       61 ggagttccgc catttgctcc ggcgtgatgt gaatttcacc cgctcgacga gcgcaacacc
      121 ggcgccgcct acgccaagaa ccggcgaatc gaactcaaac tcacgaaccg ctgagcgccg
      181 cgtcaccgcg ctgctgcgcc agccagtcgc gcggggcgca gcccatgcgc acgcggaagg
      241 cgcgcgccag agcgttcggg ctgccgtagc cgacgcggtc ggccaccacc gccaccggcc
      301 gcccctgctt gagcagggtg caactgacgt tcatccgcca gtcggccaga tagtcgcccg
      361 gcgtgacgcc caccgcgtcc ttgaacgcgg cggcgaagcg cgcgcgtgac atgcccgcct
      421 tcgccgccag cgcctcgagc gaccacgcgg tctgcggcgc atcgtgcatg gcggttatgg
      481 cgcgcgcgag tttcgggtcg gccaggcccg ccagcagtcc ggtggcgcca agccgcccat
      541 ccatcagata acgcagcaac tggatcagta gcaactcgca caagcggtcg atggcggcct
      601 gccgaccgca ctggtcgcgc tcggcttcgg caaacagcaa ctctagcgtc ggcccgaggc
      661 cgggcaagtc cgccaacgga atcagcagca tcgacggcaa ggccatggcc aacggattgc
      721 ccgtcgaagc gcccagatcg acctccgcac acaacagctc agcggtatcg ccgggggcgg
      781 cgacaaaacg atgcgacgcc acgcgcggat aaaacagcag gctcggctcg gtaaccagca
      841 gatcttcatg cacgggcgaa cgcgccgtaa tcgggccgcg ccgcaccaga tggatatagc
      901 catgcggcgc cgcgtaatga tgctgcccac agaaggcgcc gctgtgaaaa acgcgcgccg
      961 aaagcgagta gtgccggagc aagccggcca gtcgatccgc cattccactc tccatataag
     1021 acgataggtt acgtatttga tactttacgt gccatatcgt accaaacaaa gggcgatcat
     1081 gcccacgtgc tcttgcacct cacccactac ggagatagac atgacccaaa tcgcccccct
     1141 gaccatcgac accgctgacg ccgctaccgc tgccacactc aaggccgtca aagccaagct
     1201 tggcatggtg ccgaacctgt tcgccacgct ggcccacgcg ccggcggcgc tcaatggcta
     1261 cctcggcttg tccgaaacgc tgggcaccgg ccgcctgaat gcctcccagc gcgagatcgt
     1321 tgcgctcgct gccggtcaag ccaatcgctg ccagtattgc ctgagcgcac acaccctgat
     1381 cggcaaaggt gccggactgt cggcagaggc catcgccgcg gcccgcaccg gccaggcggc
     1441 caacgcactt gacgatgcaa tcgccggctt tgcccgcgcg ctggtcgagc aacgcggcgt
     1501 ggtgtcggcc gacgccatgg ccaactaccg ccgcgcgggg ctcgacgacg gcctgatact
     1561 ggaagtgatc gccaacgtcg cgctgaacac gctcaccaac tacaccaacc acatcgccga
     1621 caccacggtg gatttccccg tggtcgccgt ctgatctcca taggcatata aggagaacac
     1681 gatgaaaatc gcactcaccg gcgctctgct cgccagcgcc ctcgtcctgc cactggccgt
     1741 cacggccggc gacttttcgc cctatgtgga cagccagggc ggcatcagcc gccccacaga
     1801 cttccgcaca aacttcgtcc acctgggctc atatgcggtg ttggacgaaa aatccgcctc
     1861 tcgcggcttg cacgatgtgt acaccgaaaa ggcctcggcc gagcactacc gcaagaccgg
     1921 caagttcttg gacggcgcca cgctggtgaa agagatccgc aagctcgaaa ccagcgccat
     1981 gaccactggc aaccctgtgg tctggggttc cgatgcggcc gtgtggttcg tgatggtcaa
     2041 ggacgccaaa ggccgtttcg ccagcaaccc cttgtggggc gacggctggg gctgggcgct
     2101 gttcaaggcc gacgcccccg ccaagaatgt cgccgtcagc tacgaagccg actgcatggg
     2161 ctgccatgtg ccggcggcca agaccgaccg cgtattcatc cagggctatc cgacactgac
     2221 ccagcactga cttgctgccg ggttacgctt cgcgcaggat ggcacctccg tcctgcgcga
     2281 gccacccttt ccggaagcgc gccatgccga cactcgacgc ctttctgacc gacgtccgcg
     2341 cctgcacgcg ctgcgcaccg catctgccac acggcgtgca gccggtcttc cagtttcacc
     2401 cgagtgcgcc catcctcatc gccggccaag caccgggggc ccgggtccat gcctcaggcg
     2461 tgcccttcga cgacgccagc ggcgcgcgtc tgcgcgcctg gctgggcgtg gaccgcgaca
     2521 ccttctacga ccctacccgc atcgccattc tgcccatggg cttctgttat ccgggcaccg
     2581 gcaaaagcgg tgacctgccg ctacgcccgg agtgcgcccc ccgcctggcg cgaagccttc
     2641 atgcagcgtt tcgagcgcct gtcgctggtc atcgtgctgg gcagctacgc catggactac
     2701 cacctcggta ctggcaagac gccgctcacc cgggtggtcg aagcctggcg cgagcactgg
     2761 ccgcaagcgt tccccctgcc tcaccctagc ccgcgcaaca atcgctggct ggtgcgcaac
     2821 ccctggttcc aacaagacgt gctgcccgcg ctgcaagcac gcgtccaggc agtcctcacc
     2881 gccaacccca aggaaacccc atgaccgacg acaccgccgc cattaccgag ctgctgcaca
     2941 agtactacga caccctggcc tgggcgaacg gagacaggca ccggctttgg agcgatgaac
     3001 gacggcttca ggtgcggtag ctgacgcctg ggacctcaat caccgtatga ccgggtcgaa
     3061 agcggtcctt tctctgacaa catcgcccta gcccgcactc aacccccaca acacatccag
     3121 cacatgttgc gtaggccgct cgacgtcccc accacggtgg gcaataccaa gttgacgcca
     3181 caataagggc tgtaatggcc gcatgacgat cctggtatcg ggcaatggcg tggaggcttc
     3241 atgcggcaac aacgtcgcac cataaccggc cgccactagg cttttgatcg catcgttgta
     3301 gttcagttga atacgcggcg tgggctgccg tccatcactg gcgaaccact ccgaggtcaa
     3361 gcgcgaaagc cgagtagttt tgtcattcag aattaatggg gtatccgtaa tcggtagttg
     3421 atttgacgcc tgtcgggcag tcatgttgtg ttg
//
LOCUS       DAOQNN010000351         3436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18797_length_3436_cov_11.846791, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000351 DAOQNN010000000
VERSION     DAOQNN010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18797_length_3436_cov_11.846791"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /gene="dxs"
                     /locus_tag="PKV97_14355"
     CDS             complement(<1..1143)
                     /gene="dxs"
                     /locus_tag="PKV97_14355"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNB07111.1"
                     /translation="MAPYPNLERVGCPADVRALDRRELGALASELRAFLIESVSKTGG
                     HLSSNLGTVELTIALHHVFNTPHDRIVWDVGHQTYGHKVLTGRREAMTGLRHFGGISG
                     FPRRCESEYDTFGTAHSSTSISAALGMAVAARARGEDRHAIAVIGDGAMSAGMAFEAL
                     NNAGDMENLNLLVILNDNEMSISPPVGALTKILARMMSGSTYNTARRVGEKVLGVAPP
                     VAELARKVEEYAKGMIGPGTLFEEFGFHYYGPIDGHDLDALIPTLQNLRKLKGPQFLH
                     VITRKGQGYKLAEADPVLYHGVSKFDHTAGIQTGKGGGKLTYTQVFGDWLCDMAKADP
                     RIVGITPAMREGSGLVRFAQEFPERYFDVGIAEQHAVTFAAGLACEG"
     gene            complement(1192..2100)
                     /locus_tag="PKV97_14360"
     CDS             complement(1192..2100)
                     /locus_tag="PKV97_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNB07112.1"
                     /translation="MNADSKLPSFSEWTAQIQQRTEVALAGVLPDAAIAPQRLHEAMR
                     YAVLGGGKRVRPLLAHAAGALAGAAAEDLDRIACAVELIHAYSLVHDDLPCMDDDVLR
                     RGKPTVHVEFDEATALLAGDALQTLAFQVLAERPLGTDPANQLAMLNQLAIASGSRGM
                     AGGQAIDLASVGKRLGREELEFMHIHKTGALIRAAVLLGARAGGALGADEHARLDHYA
                     KVVGLLFQVVDDLLDAEADTATLGKTAGKDADNDKPTYVSLLGLVDARRLAEEMLDEA
                     RASLRPFGARALRLEQLADYIVHRAF"
     gene            complement(2097..2351)
                     /locus_tag="PKV97_14365"
     CDS             complement(2097..2351)
                     /locus_tag="PKV97_14365"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNB07113.1"
                     /translation="MPKSASSPKSFEAAVSELEAIVQEMESGNLPLEDALARYQRGVG
                     LLRHCQTTLSDAEQRVRMLEGDGLTDFPTEPTRDGDEAGA"
     gene            2478..>3436
                     /locus_tag="PKV97_14370"
     CDS             2478..>3436
                     /locus_tag="PKV97_14370"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNB07114.1"
                     /translation="MSDIASKARLAQAASQLPVSWYFDQQVFELEKKLLFDAGPGYVG
                     HELMVPEVGNYRSLEWLDHAKLLFRTEAGVHQMSNVCRHRQAIMLQGSGTTKLVVCPV
                     HRWTYDRQGNLLGAPHFPEKPCLGLRRDELERWNGLLFKGPRSASADLAGMQVADEFD
                     FSGYKLDKVEVHHCNYNWKTFIEVYLEDYHVVPFHPGLGNFVTCKDLSWQFGDWYSVQ
                     KVGITSLAKPGSETYAKWHKAVIDYYGEKKPTHGAIWLTYYPNVMVEWYPHVLVVSTL
                     IPTDVDKTTNVVEFYYPEDIVEFEREFVEAEQAAYMETAIEDD"
BASE COUNT          559 a         1081 c         1218 g          578 t
ORIGIN      
        1 gccctcgcag gccagcccgg cggcgaaggt gaccgcgtgc tgctcggcga tgccgacgtc
       61 gaagtagcgc tcggggaact cctgcgcgaa gcgcaccagg ccggagccct cgcgcatcgc
      121 cggggtgatg ccgacgatgc gcggatcggc cttggccatg tcgcacagcc agtcgccgaa
      181 cacctgggtg taggtgagct tgccaccgcc cttgccggtc tggatgccgg cggtgtgatc
      241 gaacttcgac accccgtggt agagcaccgg gtcggcctcg gcgagcttgt agccctggcc
      301 cttgcgggtg atcacgtgga ggaactgcgg tcccttgagc ttgcgcaggt tctgcagggt
      361 cggaatcagt gcgtcgaggt cgtggccgtc gatcggaccg tagtagtgga agccgaactc
      421 ctcgaacagc gtgcccgggc cgatcatgcc cttggcgtac tcctcgacct tgcgcgcgag
      481 ctcggccacc ggcggggcga cgccgagcac cttctcgccg acccggcgcg cggtgttgta
      541 ggtcgagccc gacatcatgc gggcgaggat cttggtgagc gcccccaccg gtggcgaaat
      601 cgacatctcg ttgtcgttga ggatcaccag caggttgagg ttctccatgt cgccggcgtt
      661 gttgagggcc tcgaaggcca tgccggccga catcgcgccg tcgccaatca ccgcgatcgc
      721 gtggcggtcc tcgccgcgtg cgcgcgccgc cacggccatg ccgagcgcag ccgagatcga
      781 ggtggaggag tgtgcggtgc cgaaggtgtc gtactcgctc tcgcagcgcc gcgggaagcc
      841 ggagatgccg ccgaagtggc gcagcccggt catcgcctcg cggcggccgg tgagcacctt
      901 gtggccgtag gtctggtggc cgacgtccca cacgatgcgg tcgtgagggg tgttgaaaac
      961 gtggtgcagc gcgatcgtca gctcgacggt gccgaggttg gaggatagat ggccgccggt
     1021 cttcgatacc gactcgatca ggaaggcgcg caattcggag gcgagggcgc cgagttcgcg
     1081 gcgatcgagt gcacggacgt cggcgggaca gccgacgcgt tccaggttcg ggtagggggc
     1141 catgggggat ccggctttgg ggttgttctt gggggccgca gtcaggcgcg atcagaaggc
     1201 gcgatgcacg atgtagtccg cgagctgctc caggcgcagc gcacgcgcgc cgaaagggcg
     1261 caggctggcg cgcgcctcgt cgagcatctc ctcggcgagg cggcgggcgt cgaccaggcc
     1321 gagcaggctc acgtaggtcg gcttgtcgtt gtcggcgtcc ttgcccgcgg tcttgcccag
     1381 cgtggcggtg tcggcctcgg cgtcgaggag gtcgtcgacc acctggaaga gcaggcccac
     1441 gaccttggcg tagtggtcca ggcgagcgtg ctcgtcggca cccaatgcgc cgccggcacg
     1501 cgcgccgagc agcaccgcgg cgcggatcag cgccccggtc ttgtggatgt gcatgaactc
     1561 gagctcttcg cgccccagcc gcttgcccac cgaggcgagg tcgatcgcct ggccgccggc
     1621 catgccgcgc gagcctgacg cgatggcgag ctggttcagc atcgcgagct ggttcgcggg
     1681 gtccgtgccg agcggacgct cggcgagcac ctggaaggcc agcgtctgca gcgcgtcgcc
     1741 ggcgagcagg gcggtggcct cgtcgaactc gacatgcacg gtaggcttgc cgcgacgcag
     1801 cacgtcatcg tccatgcaag ggagatcgtc gtgcaccagc gaataggcgt ggatcagctc
     1861 gaccgcgcag gcgatgcggt cgaggtcctc ggcagcggcg ccggcgagtg cgccggccgc
     1921 atgcgcgagc agcggacgca cgcgcttgcc accgcccagc accgcgtagc gcatcgcctc
     1981 gtgcaggcgc tgcggggcga tcgcggcatc gggcagcacg ccggcgagcg cgacctcggt
     2041 gcgctgctgg atctgcgccg tccattcgga gaaggagggg agcttgctgt ctgcgttcat
     2101 gcgcctgcct cgtcgccgtc gcgggtcggt tcggtcggga aatcggtgag gccgtcgcct
     2161 tcgagcatgc gcacgcgctg ctcggcgtcg ctgagcgtgg tctggcagtg acgcagcagg
     2221 ccgacgccac gctgatagcg cgcgagggca tcctcgagcg gcagattgcc ggattccatt
     2281 tcctggacaa tggcctcgag ctcggagacc gccgcttcga acgacttggg ggaggatgcc
     2341 gactttggca tggatcgata tctggcgcgg caaaagcgga aaaataaccg aaggcgggta
     2401 tggcggtcaa acggggggtg tcttacacta aaccctttat ttttccggat ttcttgtccg
     2461 caagaggagg ttcggggatg tccgacatcg cttccaaggc tcgactggcc caggcagcct
     2521 cccagctgcc tgtctcgtgg tacttcgacc agcaggtttt cgaactggag aagaaactcc
     2581 tcttcgatgc cggtccgggg tacgtcggcc atgagctgat ggttcccgag gtcggcaact
     2641 accgctcgct ggagtggctc gaccacgcca agctgctgtt ccgcaccgag gcgggtgtgc
     2701 accagatgtc caacgtctgc cgccaccgcc aggcgatcat gctgcaaggc agcggcacga
     2761 ccaagctcgt ggtctgcccg gtgcaccgct ggacctacga ccgccagggc aacctgctcg
     2821 gcgcaccgca cttccccgag aagccctgcc tgggcctcag gcgcgacgag ctcgaacgct
     2881 ggaatggcct gcttttcaag ggcccgcgct cggcgagcgc cgaccttgcc gggatgcagg
     2941 tggcggacga attcgatttc tcgggctaca agctcgacaa ggtcgaggtc caccattgca
     3001 attacaactg gaagaccttc atcgaggtct atctggagga ctatcacgtc gtgcccttcc
     3061 accccggact gggcaacttc gtgacctgca aggacctgag ctggcagttc ggcgactggt
     3121 attcggtaca gaaggtgggc atcacctcgc tcgccaagcc gggctcggag acctacgcca
     3181 agtggcacaa ggcggtgatc gactactacg gcgagaagaa gccgacgcat ggtgcgatct
     3241 ggctcaccta ctacccgaat gtcatggtgg agtggtaccc gcacgtgctg gtggtgagca
     3301 cgctgattcc gacggatgtg gacaagacga ccaacgtggt ggagttctac tacccggagg
     3361 acatcgtcga attcgagcgc gagttcgtcg aggccgagca ggccgcctac atggagaccg
     3421 cgatcgagga cgacga
//
LOCUS       DAOQNN010000352         3424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18899_length_3424_cov_9.571386, whole genome shotgun sequence.
ACCESSION   DAOQNN010000352 DAOQNN010000000
VERSION     DAOQNN010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18899_length_3424_cov_9.571386"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PKV97_14375"
     CDS             complement(<1..818)
                     /locus_tag="PKV97_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HNB07115.1"
                     /translation="MTSERPRSRPLRGVANTAVFLRAVDSTLRAIAGCAGGASAPSGV
                     DGIETAALPPALTTAEIVRLRGEADALALRLRHHDERLHALHDLQGERARAAYDAIAQ
                     ARVEALGAGRMPGVAANIAALIEQRCRDRPLDRATSREQVALAEALHVLARERFCGAP
                     PPPAARTMAELWRPYFDAHVGPRLDGLREHLRDERAFAAGLHELIEALDLENPTSEAR
                     EPRKLARSEGEPGDEDAAEQARAGGAAARDAQPSLRHASSGRNEGGRAARADFAA"
     gene            complement(829..1821)
                     /gene="cobS"
                     /locus_tag="PKV97_14380"
     CDS             complement(829..1821)
                     /gene="cobS"
                     /locus_tag="PKV97_14380"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HNB07116.1"
                     /translation="MTEIPMTAAGPLPPLPDTECPVREAFGLDSDMRVPAFSRGSEHV
                     PPRDDTYRFDPDTTLAILAGFAFNRRVMIQGYHGTGKSTHIEQVAARLNWPCVRINLD
                     SHVSRIDLIGKDAIVLRDGKQVTEFREGILPWALQHACALVFDEYDAGRPDVMFVIQR
                     VLEVEGRLTLLDQSRVIHPHPAFRLFATANTIGLGDTTGLYHGTQQINQAQMDRWNIV
                     ATLNYLDHDAEVEIVVAKMPAYDTKAGRARVGAMVMLAGLTRSGFIDGDLSTVMSPRT
                     VLTWAENALIFADLRFAFRVSFLNRCDEIERAVVAEYYQRCFGEELIDPRAAAG"
     gene            1996..2451
                     /locus_tag="PKV97_14385"
     CDS             1996..2451
                     /locus_tag="PKV97_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB07117.1"
                     /translation="MADELGFHLRCAQLAAFKHFAHAVGAVEGITPGLYGMLQAIDNN
                     PGLSQSALAVAMDVDRSSIVKVVDRLEGKGLIVRDASPTDRRRYRLHMTATGARALRR
                     IQDAVIRQDRVFSARLDDTERATLIALLTRLYRPDSETPTARAGIGAES"
     gene            2448..2933
                     /gene="hcrC"
                     /locus_tag="PKV97_14390"
     CDS             2448..2933
                     /gene="hcrC"
                     /locus_tag="PKV97_14390"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit gamma"
                     /protein_id="HNB07118.1"
                     /translation="MKNILRLTLNGRLREDLVPDNMLLLDYLREAVGLTGTKQGCDGG
                     ECGACTVLVDDRPRLACSTLAHQVAGRKVETVESLSTHGTLSKLQAAFHEKLGTQCGF
                     CTPGMLMASEALLRKNPDPSREEIKTALAGNLCRCTGYVKIIESVEAAAAARLCAEEA
                     K"
     gene            2930..>3424
                     /locus_tag="PKV97_14395"
     CDS             2930..>3424
                     /locus_tag="PKV97_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit alpha"
                     /protein_id="HNB07119.1"
                     /translation="MNPRLPRNGTVGARTPLVDGVEKVTGKAKYTADIAAPGALVGRI
                     LRSPHAHARILSIDTAAAEALEGVFAVCTGAETPVPYGVLPIAENEYPLAREKVRYRG
                     DPVAAVAAIDEATAEKALGLIRVDYEVLPAYFTPKAARKAGAVAIHDDKPDNVLREVH
                     AEFGD"
BASE COUNT          534 a         1157 c         1207 g          526 t
ORIGIN      
        1 gcggcaaagt ctgcccgcgc tgcccgcccg ccctcgttcc gaccggacga ggcgtggcgg
       61 agcgagggct gcgcgtccct cgcagccgcg ccgcccgccc gcgcctgctc cgcagcgtcc
      121 tcgtccccgg gttcgccctc gctgcgggcc agcttgcgcg gctcgcgggc ttcgctggtg
      181 gggttttcca ggtcgagcgc ctcgatcagt tcatgcaatc ccgcggcgaa cgcgcgttcg
      241 tcgcgcaggt gctcgcgtag gccgtcgagg cgcgggccga cgtgggcgtc gaagtagggt
      301 cgccacagtt cggccatggt ccgtgccgcc ggcgggggag gcgcgccgca gaagcgttcg
      361 cgggcgagga cgtgcagggc ctcggcgagc gcaacctgtt cccggctggt cgcgcggtcg
      421 agcggcctgt cccggcagcg ctgctcgatg agggcggcga tgttggccgc cacgcccggc
      481 atccggcccg cccccaaggc ctcgacgcgt gcctgtgcga tcgcatcgta ggccgcgcgt
      541 gcgcgctcgc cctgcagatc gtgcagggca tggaggcgct cgtcgtgatg gcgcaggcgc
      601 agggccagcg cgtcggcctc gccgcgcagg cggacgatct ccgccgtcgt cagggcgggc
      661 ggcagtgcag cggtttcgat gccgtcgacg ccggatgggg cggacgcgcc gccggcgcac
      721 ccggcgatcg cacgcagggt gctgtcgacc gcccgcagaa agacggccgt gttcgcgact
      781 ccgcgcaggg gccgggagcg ggggcgttcg cttgtcatga ggctgcgatc agcccgcggc
      841 ggcgcgcgga tcgatcagct cctcgccgaa acagcgctgg tagtactcgg ccaccaccgc
      901 gcgctcgatc tcgtcgcacc ggttcaggaa gctgacccgg aatgcgaagc gaaggtccgc
      961 gaagatcagc gcgttctcgg cccaggtcag caccgtgcgc ggcgacatga ccgtcgacag
     1021 gtcgccgtcg atgaagccgc tgcgggtcag tccggccagc atcaccatgg cgccgacgcg
     1081 ggcgcgtccg gctttcgtgt cgtatgcggg catcttggcg acgacgatct ccacctcggc
     1141 atcgtggtcg aggtagttca gggtcgcgac gatgttccag cggtccatct gggcctggtt
     1201 gatctgttgg gtgccgtgat agagcccggt ggtgtcgccc aggccgatgg tgttggcggt
     1261 ggcgaacagg cgaaacgccg gatgcggatg gatcacccgc gactgatcga gcagcgtgag
     1321 cctgccctcg acctccagta cgcgctggat cacgaacatc acgtcgggcc ggccggcgtc
     1381 gtattcgtcg aagaccagtg cgcaggcatg ttgcagggcc cagggcagga tgccctcgcg
     1441 gaactcggtc acctgcttgc cgtcgcgcag cacgatggcg tccttgccga tcaggtcgat
     1501 gcggctgacg tggctgtcga ggttgatgcg cacgcacggc cagttcaggc gtgcggccac
     1561 ctgctcgatg tgggtcgatt ttccggtgcc gtgatagccc tggatcatca cccgccggtt
     1621 gaaggcgaag cccgccagga tggccaacgt cgtatcggga tcgaagcggt aggtgtcgtc
     1681 gcgtggcggc acatgctcgc tgccccggct gaaggccggc accctcatgt cgctgtccag
     1741 cccgaaggcc tcgcgcactg gacattcggt atcgggcagg ggcggaagag ggcctgcggc
     1801 tgtcatcggg atttcggtca tcgagggctc ccgggtgtgg aggaggcaag ggcggtcctg
     1861 gcgaatcggc aaacaatcca gttgcagaaa aagtttgttg gagcaacaat tggaacacaa
     1921 acaatttgag gcgccaatag catgcccccg gatgatccga acccaggcaa gccggcagtg
     1981 gtttcccatg gtcccatggc cgacgaactc ggcttccacc tgcgctgcgc gcagcttgcc
     2041 gcgttcaagc acttcgccca tgccgtcggt gcggtggaag ggatcacacc cggcctctac
     2101 ggcatgctcc aggcgatcga caacaacccc gggctgagcc aaagcgcgct ggcggtcgcg
     2161 atggatgtcg accgctcctc gatcgtcaag gtcgtcgatc ggctggaggg gaaaggcttg
     2221 atcgtccgtg acgcctctcc gaccgatcgc aggcggtacc ggctgcacat gacggcgacc
     2281 ggcgcgcggg cgctacggcg tatccaggac gccgtgatac gccaggaccg ggtcttctcg
     2341 gcgcgcctcg acgacaccga gcgcgccacg ctgatcgcgc tgctgacccg actttaccga
     2401 ccagacagtg aaaccccgac cgctcgcgcg ggaataggag ccgaatcttg aagaacatcc
     2461 ttcgcctgac cctcaacggg cgtctccgtg aagacctcgt ccccgacaac atgctgctgc
     2521 tggattacct gcgcgaagcg gtcggcctca ccgggaccaa gcagggctgc gatggcggcg
     2581 aatgcggcgc ctgcaccgtc ctggtggacg atcgcccccg cctcgcctgt tccaccctgg
     2641 cccaccaggt cgccggccgg aaggtcgaga ccgtcgagtc gctgtcgacc cacggcacgc
     2701 tctcgaaact gcaggccgca ttccacgaga aactgggtac ccagtgcggc ttctgcacgc
     2761 cgggcatgct gatggcgtcc gaagccttgc tgcgcaagaa ccccgatccc tcgcgcgagg
     2821 agatcaagac ggcgctggcc ggcaatctgt gccgctgcac cgggtacgtg aagatcatcg
     2881 agtccgtgga ggccgcggcg gcggcccggc tgtgcgcgga ggaggcgaaa tgaacccgag
     2941 gctccccagg aatggcacgg tcggcgcccg cacgcccttg gtcgacgggg tggagaaggt
     3001 caccggcaag gccaaataca cggcggacat cgccgccccc ggcgccttgg tcggacgcat
     3061 cctgcgctcg ccgcacgccc acgcccgcat cctgtccatc gacaccgcgg cggccgaggc
     3121 gctggagggg gtgttcgcgg tctgcaccgg tgccgagacc ccggtgccct acggcgtgct
     3181 gccgatcgcc gagaacgaat acccgctcgc gcgcgagaag gtgcgctatc gcggcgatcc
     3241 ggtcgcagcg gtcgccgcga tcgacgaggc gacggcggag aaggccctcg gcctgatccg
     3301 ggtcgactac gaggtcctgc ccgcgtattt cacccccaag gcggcgcgca aggcgggggc
     3361 ggtcgcgatc cacgacgaca agcccgacaa cgtgctgcgc gaggtgcacg ccgagttcgg
     3421 cgac
//
LOCUS       DAOQNN010000353         3421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18921_length_3421_cov_7.461378, whole genome shotgun sequence.
ACCESSION   DAOQNN010000353 DAOQNN010000000
VERSION     DAOQNN010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18921_length_3421_cov_7.461378"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..986)
                     /gene="serB"
                     /locus_tag="PKV97_14400"
     CDS             complement(141..986)
                     /gene="serB"
                     /locus_tag="PKV97_14400"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNB07120.1"
                     /translation="MNLVVLAEDVDSVALKNVAKVSEASAIEQITTRSFRLVGAKPVE
                     GLVAACAAGGLDHVWLPEGRQLRDFGLFVTDMDSTLINIECIDEIADMQGFKREVAAI
                     TEAAMQGEIDFRESLTRRVALLAGLPESALAEVFDHRLQLNPGAERLLQGLQRAGIRT
                     MLVSGGFTYFTERLKSRLGFDYAWANQLEVYDGKLTGKVLGDIVDGEAKAAHLEQVRD
                     ELGLRPEQVIAAGDGANDIPMLRAAGFGVAFHAKPVLRDVAHCCLDHTGLDGILDLFG
                     PGGES"
     gene            complement(983..2059)
                     /locus_tag="PKV97_14405"
     CDS             complement(983..2059)
                     /locus_tag="PKV97_14405"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNB07121.1"
                     /translation="MPTQTENLNVLAFDHMPSPDEVKARVPLTERAAAAVVAGRKAVM
                     DILDRKDPRVFVVVGPCSIHDPVAGLDYARRLKALADEVSDVLLLVMRVYFEKPRTST
                     GWKGYINDPFMDDSFRIDVGMERARKFLLDVCELGLPTATEALDPIAPQYYGDLIAWT
                     AIGARTSESQTHREMASGLSTPVGFKNATDGDLEVAINAIISAGSPHSFLGINSQGQS
                     AVTRTRGNRYGHVVLRGGGGRPNYDTVSVSLAEQALAKAKLAKNIVVDCSHANSWKKP
                     EYQPLVMKDVMHQIREGNQSIVGLMIESNIEAGNQPIPADLSQLKYGCSVTDACVDWA
                     TTEDMIRKSAAVLRDVLPKRERRA"
     gene            complement(2202..2585)
                     /locus_tag="PKV97_14410"
     CDS             complement(2202..2585)
                     /locus_tag="PKV97_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB07122.1"
                     /translation="MKQVQAIRPDREVLTRQRLPYFIGISGQTVGASGLSMHLVTIPP
                     GARSEPHVHVGYETGIYVLEGRVLTRWGAKLENELISAAGEFLFVPAGVPHEAINLSA
                     TEAARAVVARNHPAEQDQVQPYVAG"
     gene            complement(2716..>3421)
                     /locus_tag="PKV97_14415"
     CDS             complement(2716..>3421)
                     /locus_tag="PKV97_14415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB07123.1"
                     /translation="RGIDVAQAHAAATISTHRKRFRAMPRIEPFEAHAARYDAWFERH
                     QAAYVSELLALRPFVPLSGRGLEIGVGSGRFAAPLGVQVGIDPSRTMLERARARGVEV
                     CEGTAEHLPFAEHSFDHALVVTTLCFVDSPAAMLTQARRVLKPGGSLVIAFIDRDSPL
                     GRDYEAHRETSDFYRDASFHSAGEVERLLLDAGFSLRSWAQTLARPLAENGHIEPARP
                     GRGEGAFVVVDAVRPS"
BASE COUNT          528 a         1205 c         1131 g          557 t
ORIGIN      
        1 gccggccgcc tcagcgaggc cccgtaggag cgacgccagt cgcgatcggg cggcgcggtg
       61 ccgctgacgc ggcggtggag gtgcgggggc cggaatcgcg actggcgtcg ctcctacgaa
      121 aacgggggcc gcgtgcggct tcagctttcg ccgccagggc cgaacaggtc gaggatgccg
      181 tccaggccgg tgtggtcgag gcagcagtgg gcgacgtcgc gcagcaccgg cttggcgtgg
      241 aaggcgacac cgaagccggc cgcgcgcagc atggggatgt cgttggcgcc gtcgccggcg
      301 gcgatcacct gctcgggcct gaggcccaac tcgtcgcgca cctgctccag gtgcgcggcc
      361 ttggcctcgc cgtccacgat gtccccgagc acctttccgg tcagctttcc gtcatacacc
      421 tcgagctggt tcgcccacgc atagtcgaag cccagccgcg acttcagccg ctcggtgaaa
      481 tacgtgaagc cgcccgacac cagcatcgtg cggatacccg cgcgctgcaa cccctgcagc
      541 aggcgctccg ccccggggtt gagctgcagg cggtgatcga agacctcggc gagcgcgctc
      601 tcgggcaggc cggcgagcag ggccacgcgc cgcgtcagcg actcgcggaa gtcgatctcc
      661 ccctgcatcg ccgcctccgt gatcgccgcc acctcgcgtt tgaagccctg catgtcggcg
      721 atctcgtcga tgcactcgat gttgatcagg gtcgagtcca tgtcggtcac gaagaggccg
      781 aaatcgcgca gctggcggcc ctcgggcagc cacacgtggt cgagcccccc ggccgcgcac
      841 gcggccacca ggccttcgac cggcttcgcg ccgacgaggc ggaaggagcg cgtcgtgatc
      901 tgctcgatcg cgctcgcttc cgacaccttg gcgacgttct tgagcgcgac gctatcgacg
      961 tcctcggcca gcacgaccag gttcatgcgc gccgctcccg cttcggcagc acgtcgcgca
     1021 gcacggcggc ggacttgcgg atcatgtcct cggtcgtcgc ccaatcgaca caggcgtcgg
     1081 tgaccgaaca gccgtacttg agctgcgaca ggtcggccgg aatcggctgg ttgccggctt
     1141 cgatattgct ctcgatcatc aggccgacga tcgactggtt gccctcgcgg atctgatgca
     1201 tcacgtcctt catcaccagg ggctggtatt cgggcttctt ccacgagttg gcgtgcgagc
     1261 aatcgaccac gatgttcttc gccagcttgg ccttcgcgag cgcctgctcg gccagcgaca
     1321 ccgacaccgt gtcgtagttg ggccggccgc cgccgccgcg cagcaccacg tggccgtaac
     1381 ggttgccgcg cgtgcgggta accgccgact ggccctggct gttgatgccg aggaagctgt
     1441 gcggactgcc ggccgaaatg atcgcgttga tcgccacctc gaggtcgcca tcggtggcgt
     1501 tcttgaagcc gaccggcgtc gacaggcccg aggccatctc gcgatgggtc tgcgactcgg
     1561 aggtgcgcgc gccgatcgcg gtccaggcga tgaggtcgcc gtaatactgc ggtgcaatcg
     1621 ggtcgagcgc ctcggtggcc gtgggcaggc cgagctcgca cacgtcgagg aggaatttgc
     1681 gcgcacgctc catgcccacg tcgatgcgga aggagtcgtc catgaacgga tcgttgatgt
     1741 agcccttcca cccggtcgag gtgcgcggct tctcgaaata cacccgcatc accagcagca
     1801 gcacgtcgga gacctcgtcg gcgagcgcct tcagccgtcg cgcataatcc agcccggcca
     1861 ccgggtcatg gatcgagcac ggcccgacca ccacgaacac gcgcggatcc ttgcggtcga
     1921 ggatgtccat caccgccttg cgcccggcca ccaccgcggc cgcggcgcgc tcggtcagcg
     1981 gcacgcgcgc cttcacctcg tccggcgagg gcatgtggtc gaaggcgagg acgttgaggt
     2041 tttcggtctg ggtgggcatt gcgggcactc cgattgctgc ctggaaagcc tgcaattgta
     2101 acgcccgtgc cggggcatgt ccgcggagat ttgcgcggcg caagggattg gccggcacgc
     2161 atcgaagccg cgccgcttga tggatccgcc gcgccgagcc cctacccggc aacgtagggc
     2221 tgaacctggt cctgctctgc cggatggttg cgggccacca ccgcgcgcgc cgcttcagtg
     2281 gcgctgagat tgatcgcctc gtgcggaacc cccgccggaa cgaacaggaa ctcccccgcc
     2341 gcactgatca gctcgttttc cagcttcgcg ccccagcgcg tcaggaccct gccttcgagc
     2401 acatagattc cggtttcgta gccgacgtgg acatgcggct ccgaccgggc cccgggcgga
     2461 atcgtcacca gatgcatgct cagcccgctt gccccgaccg tctgccccga gatcccgatg
     2521 aaatacggca gccgctgacg cgtcaggact tcacggtccg gtcgtatcgc ttgcacttgc
     2581 ttcatgtcgt tctcctccgc gcagggacga tcccttggaa gtgggtgtcg cgcatgttgt
     2641 ggatgccttt ccgccgggaa gattccgatg tcgccctccc gcgcgctgcc gtcctcatcg
     2701 gtggccggcg acagatcacg agggccgcac cgcatcgacg accacgaagg cgccttcgcc
     2761 ccgaccgggg cgcgccggct cgatgtgccc gttctccgcg agcggccggg ccagcgtctg
     2821 cgcccacgac cgcagggaaa aacctgcatc cagcagcagg cgctccactt cgcccgccga
     2881 atggaagctc gcatcgcggt agaaatcgct ggtctcgcgg tgcgcctcgt agtcgcgacc
     2941 cagcgggctg tcccggtcga tgaacgcgat caccaggctg ccgcccggct tcaagacccg
     3001 ccgcgcctgc gtcagcatcg ccgccggcga atcgacgaag cacaaggtgg tgacgacgag
     3061 cgcgtgatcg aaactgtgct cggcgaaagg cagatgctcg gcggtgccct cgcagacttc
     3121 gaccccgcgg gcacgcgcgc gctccagcat cgtccgcgac gggtcgatcc ccacctgaac
     3181 ccccagcggc gcggcgaagc gcccgctgcc gacgccgatc tccagaccgc gcccgctcag
     3241 cgggacgaag ggtcgcagcg cgagcagttc ggagacatag gctgcctggt ggcgttcaaa
     3301 ccacgcgtcg taacgcgctg catgcgcttc aaacggctcg atcctgggca tcgcgcggaa
     3361 cctcttccgg tgtgtcgaaa tcgtggcggc ggcgtgcgcc tgtgccacat cgatccctct
     3421 c
//
LOCUS       DAOQNN010000354         3420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_18931_length_3420_cov_11.905795, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000354 DAOQNN010000000
VERSION     DAOQNN010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18931_length_3420_cov_11.905795"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2057)
                     /locus_tag="PKV97_14420"
     CDS             complement(<1..2057)
                     /locus_tag="PKV97_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNB07124.1"
                     /translation="MLPGWLIVGASFAYLLLLFAVAYFGDRRADAGRSVIDNPWVFSL
                     SLAVYCTAWTYFGSVGRAASGGPWFLPTYLGPTLGLTLAWLVVLKMIRIARSYRVTSI
                     ADFVASRYGKSHLLGGLVTLIAVVGIVPYIALQLKAISSGYALLVGEHDPLFRIESQA
                     GWWQDGTLYIALALAAFTVLFGTRHLDTTERHEGMVAAIAFESVVKLFAFLAVGIYVT
                     YWLYDGFGDLYARAAAVPELHRLLAFDGAGRLGYGGWFAHVLLAMLSIIFLPRQFQIA
                     VVENVNEQHLRRATWVFPAYLLAINIFVIPIAIGGLLHFGRGTVDPDTFVLTLPLAHG
                     QSALALLVFIGGLSAATGMVIVEAIALSTMVCNDLVMPMLLRSRRLDLARERDLTGLL
                     LGIRRGAIVVLLLLGYLYFRLAGEAYALVSIGLISFAAVAQFAPVVIGGMYWRGGTRE
                     GALAGLLAGFAVWVYTLLLPSFAKSGWLDPGFLEHGVLGLEWLKPEQLFGLTGLDNIS
                     HALFWSLLANIGCYVAVSMLRVPTGPEATQGALFVDVFRSGAAAPASFWRTGAEAGEL
                     VPLVARFLGQRRTEEAFGAYARSHGLQQVSELKADPELVHFAETLLAGAIGSASARVM
                     VSTVVQAEPLGLGEVMDILDEASQVRAYSHKLEEKSRALEAATAELRAANERLKELDR
                     LKD"
     gene            complement(2071..2268)
                     /locus_tag="PKV97_14425"
     CDS             complement(2071..2268)
                     /locus_tag="PKV97_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07125.1"
                     /translation="MRKGLAGQRLVAVFIAGLVLLNYPILSLFDRPQTVLGLPLLHVY
                     LFSVWVALILVVAWIVERGAR"
     gene            complement(2464..>3420)
                     /locus_tag="PKV97_14430"
     CDS             complement(2464..>3420)
                     /locus_tag="PKV97_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB07126.1"
                     /translation="TYPDAGRFWKMIQDHKVSIFYTAPTAIRSLIKAADNNPAVHPKQ
                     YDLSSLRILGSVGEPINPAAWEWYYENVGGGRCPIVDTFWQTETGAHMITPMPGATPM
                     VPGSCTLPFPGIMAAVVDETGTEVPWGQGGILVVTKPWPSMIRNVWGDPDRFKKTYYP
                     EDFKGKLYLAGDGAIRDAETGYFTITGRIDDVLNVSGHRMGTMEIESALVAHEKVAEA
                     AVVGRPDDLTGEAIVAFVVLKGARPTGEDAAKMIKELQNWVGHEIGPIAKPKDIRFGE
                     NLPKTRSGKIMRRLLRQLAKGEDVTQDTSTLENPHILEQLKG"
BASE COUNT          599 a         1165 c         1191 g          465 t
ORIGIN      
        1 tcgtccttga ggcggtcgag ctccttcaag cgctcgttgg cggcgcgcag ctcggcagtc
       61 gcggcctcga gcgcacgcga cttctcctcg agcttgtgcg agtaggcgcg cacctgcgag
      121 gcctcgtcga ggatgtccat gacctcgccg agcccgagcg gctccgcctg caccacggtc
      181 gacaccatca cccgcgcgga ggcgctgccg atcgcgccgg cgagcagggt ctcggcgaag
      241 tgcaccagct cggggtcggc cttgagctcg gagacctgct gcaggccgtg cgagcgcgcg
      301 taggcgccga aggcctcctc ggtgcggcgc tgcccgagga agcgcgccac cagcggcacc
      361 agctcgccgg cctcggcgcc ggtgcgccag aagctcgccg gcgccgccgc gccgctccgg
      421 aacacgtcca caaagagcgc gccctgggtc gcctccggcc cggtcggcac gcgcagcatc
      481 gataccgcga cgtagcagcc gatgttggcg agcaggctcc agaacagcgc gtggctgatg
      541 ttgtccaggc cggtgaggcc gaagagctgc tcgggcttga gccattccag gccgagcacg
      601 ccgtgctcga ggaagcccgg atcgagccag cccgacttgg cgaacgaggg cagcagcagc
      661 gtatagaccc acaccgcgaa gccggcgagc agaccggcga gcgcgccctc gcgggtgccg
      721 ccgcgccagt acatgccgcc gatcaccacc ggggcgaact gcgccaccgc ggcgaagctg
      781 atgaggccga tgctgaccag cgcgtaggcc tcgccggcga ggcggaagta gaggtagccg
      841 agcagcagca gcaccacgat cgcaccgcgg cggatgccca gcagcaggcc ggtgaggtcg
      901 cgctcgcgcg cgaggtcgag ccgccggctg cgcagcagca tcggcatcac caggtcgttg
      961 cacaccatgg tggagagcgc gatcgcctcc acgatcacca tgccggtcgc cgccgacagg
     1021 ccgccgatga agacgagcag cgcgagcgcg ctctgcccgt gcgcgagcgg cagggtcagc
     1081 acgaaggtgt ccggatccac ggtgccgcgg ccgaaatgca gcagcccgcc gatcgcgatc
     1141 gggatcacga agatgttgat cgccagcagg taggcgggaa agacccaggt cgcgcggcgc
     1201 aggtgctgct cgttgacgtt ctcgaccacc gcgatctgga actgccgcgg caggaagatg
     1261 atggacagca tggccagcag cacgtgagcg aaccagccgc cgtagccgag ccggccggca
     1321 ccgtcgaagg cgagcaggcg gtgcagctcg ggcacggcgg cggcgcgcgc gtagaggtcg
     1381 ccaaagccgt cgtagagcca gtaggtgacg tagatgccga ccgccaggaa ggcgaaaagt
     1441 ttgaccaccg actcgaaggc gatcgccgcg accatgccct cgtggcgctc ggtggtgtcg
     1501 aggtggcggg tgccgaacag caccgtgaag gcggcgagcg cgagcgcgat gtagagcgtg
     1561 ccgtcctgcc accagcccgc ctggctctcg atgcggaaca gcgggtcgtg ctcgcccacc
     1621 agcagcgcgt agccgctgga gatcgccttc agctgcaacg cgatgtaggg cacgatgccg
     1681 accaccgcga tcagcgtgac cagcccgccg agcaggtggc tcttgccgta gcgcgaggcg
     1741 acgaagtcgg cgatcgaggt gacccggtag ctgcgcgcga tgcggatcat cttgagcacg
     1801 accagccagg ccagggtcag gcccagcgtg ggcccgagat aggtgggcag gaaccacggc
     1861 ccgcccgagg cggcgcggcc gacgctgccg aaataggtcc acgcggtgca atacaccgcg
     1921 agcgaaagac tgaaaaccca gggattgtcg atgaccgagc gcccggcatc cgcgcgccgg
     1981 tcgccgaagt aggccaccgc gaacagcagc agcagatagg cgaacgaagc gccgacgatc
     2041 agccagcccg ggagcatccc gacctccgca tcagcgcgcc ccgcgctcga cgatccacgc
     2101 cacgaccagg atcagcgcga cccacaccga gaacagatag acgtggagca gcggcaaccc
     2161 gagaacggtc tgcggccggt cgaacaggga cagtatggga tagttgagca acaccaggcc
     2221 ggcgatgaac acggccacca gccgctgccc ggccagaccc ttgcgcatcc tgtcgctccc
     2281 atgaaacgag ccacggcaaa cgaaaaggcg cagcgccgcc ctgctcttcg tggcagggtc
     2341 ggcgcgacgc acgatcaggg gcacgccttg cgcgtacccg gtctcccccc ggcaccggct
     2401 cgctggccgg cttcctcctg tcgttcagga aggccggacg cggggcgccc ggccggcact
     2461 gcatcagccc ttgagctgct cgaggatatg cgggttctcg agcgtcgagg tgtcctgggt
     2521 gacatcctcg cccttggcga gctggcgcag caggcggcgc atgatcttgc cggagcgggt
     2581 cttgggcagg ttctcgccga aacggatgtc cttcggcttg gcgatcggcc cgatctcgtg
     2641 acccacccag ttctgcagtt ccttgatcat cttcgcggca tcctcgccgg tcggacgcgc
     2701 gcccttgagc accacgaagg ccacgatcgc ctcgccggtc aggtcgtccg gacgaccgac
     2761 cacggcggcc tcggccacct tctcgtgcgc gaccagggcc gactcgattt ccatcgtgcc
     2821 catgcggtgg ccggagacgt tcagcacgtc gtcgatacga ccggtgatgg tgaagtaacc
     2881 ggtctcggcg tcacggatgg cgccgtcgcc cgcgaggtac agcttgccct tgaagtcctc
     2941 ggggtagtag gtcttcttga agcggtcggg atcgccccag acgttgcgga tcatcgacgg
     3001 ccacggcttg gtgacgacga ggatgccgcc ctgaccccag ggcacctcgg taccggtctc
     3061 gtccaccacc gcagccatga tgcccgggaa gggcagcgtg cacgagcccg gaaccatcgg
     3121 cgtggcgccc ggcatcggcg tgatcatgtg cgcaccggtc tcggtctgcc agaaggtatc
     3181 gacgatcggg cagcggccgc cgccgacgtt ctcgtagtac cactcccagg ccgccgggtt
     3241 gatcggctcg ccgaccgaac ccaggatgcg cagcgaggac aggtcgtact gcttcggatg
     3301 cactgccggg ttgttgtcgg cggccttgat cagcgaacgg atcgcggtcg gcgcggtata
     3361 gaagatgctg accttgtggt cctggatcat cttccagaag cggccggcgt ccgggtaggt
//
LOCUS       DAOQNN010000355         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_19077_length_3404_cov_8.213795, whole genome shotgun sequence.
ACCESSION   DAOQNN010000355 DAOQNN010000000
VERSION     DAOQNN010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19077_length_3404_cov_8.213795"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="PKV97_14435"
     CDS             <1..1311
                     /locus_tag="PKV97_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB07127.1"
                     /translation="PLLARDLARLLKTSDGDRHVNILFNPDFFDSDDGSPLFDGVYKK
                     VREPLDLLLGARTRGAEQQGLGEVGEDLAGYHTVSLHDGAWRLFLLRDARDRQWILVG
                     EREDVRGELLGRITLRSVLPDLVGLPLVAVLVWLAIGWGLRPLARIVESLQARGPDDL
                     SALALQDVPQELEPMVAALDRLLHQVNELLERERRFLAYAAHELRTPLAVLRIHAQNA
                     LQAPDPADREEALRLLGSGIERATRVVAQLLTLARLEPDASRPKRLPIELLALVREQL
                     AELTPLADEHGQDLALEADEGADFHLLGDAGSLGILMQNLVGNAVRHTPPDGCIRVLL
                     EATPAAIVLRVQDSGHGVPPELREKVFERFFRAGGGQGAGLGLAIVARIVELHGGTIA
                     LDGCALGGLEVRVVLPRDAAVPRGVQGDEGTVPPCSTSGGAARP"
     gene            1436..1954
                     /locus_tag="PKV97_14440"
     CDS             1436..1954
                     /locus_tag="PKV97_14440"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNB07128.1"
                     /translation="MPSNEDFHAGERALQQRWGTASLWDAARRERLLWDHLPDWVQPR
                     LAGAPFFFLATSDATGRCDCSFKGGGPGLVRILDARRLAFPDFDGNGAFMSLGNLRQN
                     PQVGCLFIDFGDGARLRVNGRATLLEGEAAARLFPGQDRAVVVDVEQVVPNCARHVPT
                     LHPPTADARGAA"
     gene            1951..2898
                     /locus_tag="PKV97_14445"
     CDS             1951..2898
                     /locus_tag="PKV97_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB07129.1"
                     /translation="MKIPYRIAANVEWTSKCNAACAMCPREAIDRPQSMSMATWRQVL
                     DRLAPDEIFRVVLAGYGEPTTHPRFFEFIDELRPHPVRFDMVSNGHLLDVERLRHLDG
                     AIDLLMVSFSSIDPDVYARVHVNLDQRRVMDNLQAANRVLRRTRLAISLTPMPDCLPA
                     LGDTIAWLRAHGIERLTMSPTLYNRGGKLEAHRLASAELRRMIRQHGLRSQELDFVPS
                     AAEIFRQWRRNRFGCIPRNVDLFVAASGDYLYCYNDVAHRHPIGHVAADPVAAVLRRR
                     EGMAALPTLCEGCNMRHRYGPRELLRAGVGFARARLREG"
     gene            3071..>3404
                     /locus_tag="PKV97_14450"
     CDS             3071..>3404
                     /locus_tag="PKV97_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07130.1"
                     /translation="MKTRTLMATAMGVLCAGLTFAQQPVAAPGEEAPVFGAQIMTEQE
                     RVEHRERMRAAGTPEEREQLRLEHHKQMVERARERGVTLPDEPPMRGGMGAGGGMGAG
                     AGMGAGGGM"
BASE COUNT          474 a         1155 c         1247 g          528 t
ORIGIN      
        1 ccgctgctcg cgcgcgatct ggcgaggttg ctcaagacca gcgacggcga ccggcatgtg
       61 aacattctgt ttaatcccga cttcttcgac agcgacgacg gcagcccgct gttcgacggt
      121 gtgtataaga aagtgcgcga gccgctcgac ctgttgctcg gcgcacgcac caggggcgcc
      181 gagcaacagg ggctcggcga ggtgggagag gatcttgcgg gctatcacac ggtgagtctg
      241 cacgatggcg cctggcggct cttcctgctc cgggacgcgc gcgaccgcca gtggatcctg
      301 gtcggcgagc gcgaggacgt gcggggagaa ctcctgggca ggatcacctt gcgcagcgtg
      361 ctgcccgatc tcgtcgggtt gccgttggtc gccgtgctgg tgtggctggc gatcggctgg
      421 ggcctgcgcc cgctcgcgcg catcgtcgaa tcgttgcagg cgcgcgggcc ggacgacctc
      481 tccgccctcg cgctgcagga cgtgccgcag gagctcgagc cgatggtggc cgcgctcgac
      541 cgcctgctgc atcaggtcaa cgagctgctc gaacgcgagc gccgcttcct cgcctatgcc
      601 gcgcacgagc tgcgcacgcc gctggccgtg ctgcgcatcc atgcccagaa tgcgctgcag
      661 gcgcctgatc cggcggatcg cgaggaggcg ctccggctgc tgggctcggg catcgagcgt
      721 gccacccggg tggtggcgca gttgctgacg ctggcccgcc tcgaacccga cgcgagccgg
      781 cccaagaggc tgccgatcga gctgctcgcg cttgtccgcg agcagctcgc cgagctgacc
      841 ccgctcgccg acgagcatgg tcaggacctc gccctcgagg cggacgaggg ggccgacttc
      901 cacctgctcg gcgatgccgg cagcctgggc atcctgatgc agaatctggt gggcaacgcg
      961 gtgcggcaca cgccgcccga cggctgcatc cgcgtgctgc tcgaggccac gcccgcagcc
     1021 atcgtgctgc gggtgcagga cagcggccat ggcgtgccgc cggagctgcg cgagaaggtg
     1081 ttcgagcgct tcttccgcgc cggtggcggg cagggggcag gtctcgggct ggcgatcgtc
     1141 gcgcgcatcg tcgaactgca tggcggcacg atcgcgctcg acggctgtgc gctcggcggg
     1201 ctggaggtgc gggtggtgct gccgcgggat gccgccgtgc cgcgcggggt gcagggcgat
     1261 gaggggacgg tgccgccgtg ctccacgtcc ggcggagcgg cccgccctta agcgtcgctt
     1321 aagacggcgt ccttaccgtg aacctgcgct ccgcgtggga gcgctcgtcg ggtgcaagtc
     1381 ggccctccgg ccgttgccga gcgcccggcg tgaacctgcg agcggggacg ggaagatgcc
     1441 atcgaatgaa gatttccatg ccggcgagcg cgccctgcag cagcgctggg gcaccgcatc
     1501 gctgtgggat gcggcgcgcc gggagcggct gctgtgggat cacctcccgg actgggtgca
     1561 gccccggctc gcgggggccc cgttcttctt cctcgccacc agcgatgcca cggggcgttg
     1621 cgactgttcc ttcaaggggg gcggccccgg cctggtgcgc atcctcgatg cgcgccgctt
     1681 ggcgttcccg gatttcgacg gcaacggcgc gttcatgagc ctgggcaacc tgcggcagaa
     1741 tccgcaggtc ggctgcctgt tcatcgactt cggcgacggc gcccgcctgc gcgtcaatgg
     1801 ccgggcgacg ctgctcgagg gcgaggcggc ggccaggctc ttccccgggc aggaccgcgc
     1861 cgtggtggtg gacgtcgagc aggtcgtgcc caactgcgcc cgccacgtcc ccacactgca
     1921 cccccctact gccgacgcgc ggggcgctgc atgaagattc cctaccgcat cgccgccaac
     1981 gtcgagtgga ccagcaagtg caacgccgcg tgcgcgatgt gtccgcgcga ggccatcgac
     2041 cgcccgcagt cgatgagcat ggcgacctgg cggcaggtgc tcgatcgcct ggcgccggat
     2101 gagatcttcc gggtcgtgct ggccggttac ggcgagccga ccacgcatcc ccgtttcttc
     2161 gagttcatcg acgagctgcg cccgcacccg gtgcgcttcg acatggtcag caacggtcac
     2221 ctgctcgacg tcgagcgcct gcgccacctc gacggtgcga tcgacctgct gatggtgtcg
     2281 ttctccagca tcgacccgga cgtctatgcc cgcgttcacg tcaatctcga ccagcgccgc
     2341 gtgatggaca acctgcaggc ggcgaaccgt gtcctgcgcc gcacccggct tgcgatcagc
     2401 ctcaccccga tgcccgactg cctgcccgcg ctgggcgaca ccatcgcgtg gttgcgcgca
     2461 catgggatcg agcggctgac catgtcgccc acgctctaca accgcggcgg caagctcgag
     2521 gcccatcgcc tggcgagcgc ggagctgcgg cggatgatcc gccagcacgg gctgcgctcg
     2581 caggagctcg acttcgtgcc gagcgcggcc gagatcttcc gtcagtggcg gcgcaaccgt
     2641 ttcggctgca ttccgcgcaa tgtcgacctg ttcgtcgccg cgagcggtga ctatctctac
     2701 tgctacaacg atgtcgcgca ccgccacccg atcggccatg tcgccgccga tccggtggct
     2761 gcggtgttgc ggcgccgcga gggcatggcg gcgctcccga ccttgtgcga gggctgcaac
     2821 atgcgccatc gctatggtcc ccgcgagctg ctgcgggcag gggtcgggtt tgcccgggcg
     2881 cgcctgcggg agggatgaag gtctggcggg cgggcgtcgt ggcagtggtg tggcggctga
     2941 cgggacggcg atacactgcg gtacacacgc cccccgcggg aagactacat ttgacgcgga
     3001 cgagcctgag agctgccatg ccctccggga gggccggcga gcaatgtcca acgtcgtaaa
     3061 ggagatgacc atgaagacgc gtacgctgat ggcaacggca atgggtgtcc tgtgcgcagg
     3121 gctgaccttc gcccagcaac ccgtcgcggc gccgggggag gaggcgcccg tcttcggcgc
     3181 ccagatcatg accgagcagg agcgcgtgga acaccgcgag cgcatgcgtg ctgcgggtac
     3241 gcccgaggag cgcgagcagc tgcgactcga gcatcacaag cagatggtcg agcgtgcacg
     3301 cgagcggggc gtcaccctcc ccgacgagcc gccgatgcgc ggcggcatgg gggcgggcgg
     3361 tggcatgggg gcgggagccg gcatgggggc gggcggtggc atgg
//
LOCUS       DAOQNN010000356         3393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_19161_length_3393_cov_9.083583, whole genome shotgun sequence.
ACCESSION   DAOQNN010000356 DAOQNN010000000
VERSION     DAOQNN010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19161_length_3393_cov_9.083583"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PKV97_14455"
     CDS             complement(<1..339)
                     /locus_tag="PKV97_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HNB07131.1"
                     /translation="MPLTKETRSLLDMAYRVGVPRFHELSVAQARHSAQKLHFAFRPE
                     APAVASTAEVPIPRPDTSALLARLYRPFGSHMDDRLPLVIFAHGGGWCIGDVASYDVA
                     CRELANGSGCA"
     gene            479..1663
                     /gene="ppk2"
                     /locus_tag="PKV97_14460"
     CDS             479..1663
                     /gene="ppk2"
                     /locus_tag="PKV97_14460"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNB07132.1"
                     /translation="MSKIEETVPVADTPSTRSRKPRTAPRAKRKSVVRPIASGDTAPD
                     FSSTGIEDFEIEQAIDAAQDSKLVAVKDILARAAADPQARTDALEAILDGASPDDIHM
                     LRRALMRADLPQGPGAHPDDELSPDWRKGGYPYRNLMSRKAYEKQKYRLQVELLKLQA
                     WVKETGQRVVILFEGRDAAGKGGTIKRFMEHLNPRGARVVALEKPSELERGQWYFQRY
                     IQHLPTAGEIVLFDRSWYNRAGVERVMGFCTPDEYNEFMRQVPEFERNLVRSGIHLIK
                     FWFSVSREEQRRRFKERESHPLKQWKLSPIDLASLDKWDEYTKAKEAMFFYTDTADAP
                     WTVVKSDCKKRARLNALRYVLHKLPYNNKDVSLVGPLDPLLVGRANVVYERGEKDAVA
                     LL"
     gene            1847..2485
                     /locus_tag="PKV97_14465"
     CDS             1847..2485
                     /locus_tag="PKV97_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07133.1"
                     /translation="MNIGKLRHKATAGLIVAALLAGPAFADKPDGAGGGKPDRVQGGK
                     PDHGGPQERGDKRPDKRSEQGNRDVRGDETRGRGGDRDGGQARGREQDRDREHRTDRA
                     GSSPRVSAYFSDRHREVVRQYYGDRFRAGDCPPGLAKKGNGCMPPGQAKKWRIGAPLP
                     RDVVWYDAPGDIVLRLGMPPEGHRFVRVAADILLISVGTGMVVDAIEDLSRL"
     gene            2607..3164
                     /locus_tag="PKV97_14470"
     CDS             2607..3164
                     /locus_tag="PKV97_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNB07134.1"
                     /translation="MSFARLARALAPAVLLATLAPNAGAADDATTRLYDHSLRRLHAD
                     ESVRLADRYAGQPVLVVNTASHCGFTRQFRELEAVHQRYRERGLKVAGFPSDDFRQEA
                     ADEAETAEVCFQNFGVTFDMYAPIHVTGTEAHPLFREIARQSAAPEWNFHKYVIDRNG
                     RVVAAFGSRTKPDAPEVIAAIEKAL"
     gene            3229..>3393
                     /locus_tag="PKV97_14475"
     CDS             3229..>3393
                     /locus_tag="PKV97_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB07135.1"
                     /translation="MKEYDLIAIGGGSGGVATARRAAEYGARVLLIEAARLGGTCVNV
                     GCVPKKVMWYA"
BASE COUNT          584 a         1213 c         1145 g          451 t
ORIGIN      
        1 cgcgcagccc gagccgttgg cgagttcgcg gcaggccacg tcgtagctcg ccacgtcgcc
       61 gatgcaccag cctccaccgt gcgcgaagat caccagcggc agacggtcgt ccatatggct
      121 gccgaagggc cggtacaggc gggcgagcag cgccgaggtg tcggggcgcg ggatcggcac
      181 ctcggcggtg gaggccaccg ccggggcttc gggccggaag gcgaagtgca gcttctgcgc
      241 cgaatgacgc gcctgcgcca ccgagagctc gtggaagcgg ggcacgccga cacggtaggc
      301 catgtcgagc agggaacggg tctctttggt caatggcatg atcgagaagg ggagtcgttt
      361 tgcacggccg ctgcgggcgc cgcgcgaagt gcgcgattct acccgcgccc cccgaggggc
      421 gcgtgccgag cgcattgcgg aggattagaa ttcgttgaaa caagggagag gaaacatcat
      481 gagcaagatc gaggaaacag tcccggtagc agacacccct tccacgcgca gcaggaagcc
      541 gcgcaccgcg ccgcgcgcga agcgcaagag cgtcgtgcgt ccgatcgcct ccggcgacac
      601 tgcgcccgac ttcagcagca ccggcatcga ggacttcgag atcgagcagg cgatcgacgc
      661 cgcgcaggac tccaagctcg tcgccgtcaa ggacatcctc gcccgcgcgg cggccgatcc
      721 gcaggcgagg accgacgccc tcgaggccat cctcgacggc gcctcgcccg acgacatcca
      781 catgctgcgg cgcgcgctca tgcgcgccga cctgccgcag ggtccgggcg cccatccgga
      841 cgacgagctc tcgcccgact ggcgcaaggg cggctacccc taccgcaacc tgatgtcgcg
      901 caaggcctac gagaagcaga agtaccgcct gcaggtcgag ctgctcaagc tgcaggcctg
      961 ggtcaaggag accggccagc gcgtggtgat cctgttcgag ggccgcgacg ccgccggcaa
     1021 gggcggcacg atcaagcgct tcatggagca cctcaacccg cgcggcgcgc gcgtggtggc
     1081 gctggagaag ccctccgagc tcgagcgcgg ccagtggtac ttccagcgct acatccagca
     1141 cctgcccact gcgggcgaga tcgtgctgtt cgaccgctcc tggtacaacc gcgccggcgt
     1201 cgagcgcgtc atgggcttct gcacgcccga cgagtacaac gagttcatgc gccaggtccc
     1261 cgagttcgag cgcaacctgg tgcgcagcgg catccacctg atcaagttct ggttctcggt
     1321 gagccgcgag gagcagcgtc gccgcttcaa ggagcgcgag tcgcacccgc tcaagcagtg
     1381 gaagctgtcg ccgatcgacc tcgcctcgct cgacaagtgg gacgaataca ccaaggccaa
     1441 ggaggcgatg ttcttctaca ccgacaccgc cgatgcgccg tggaccgtcg tcaagtccga
     1501 ctgcaagaag cgcgcgcgcc tcaatgcgct gcgctacgtg ctgcacaagc tgccttacaa
     1561 caacaaggac gtctcgctcg tcggcccgct cgatcccttg ctggtaggcc gtgccaacgt
     1621 ggtgtacgag cgcggcgaga aggacgccgt ggcgctgctg taagcgtctg cagtccgacg
     1681 cagcgcacat ttgcatccgg gatcgacggc tggtgacaag aggtaagccg tcgtgcgggc
     1741 cgcgtccgtg gcgatctatc ctgcaggcac gggtcgccgg cgggatgtcc ctcgctggct
     1801 ggccgccccc gcgcgcccca cggcacaaac gacaagggag aacaagatga acatcggcaa
     1861 acttcgccac aaggccaccg ccggcctgat cgtcgccgcg ctcctcgccg gcccggcctt
     1921 cgccgacaag ccggacgggg ccggcggcgg caaacccgat cgcgtccagg gcggcaaacc
     1981 cgatcacggc ggtccccagg aacgcggcga caagcgcccc gacaaacgca gcgagcaggg
     2041 caatcgggac gtgcgtggcg acgagacgcg cgggcgcggt ggagaccgcg acggtggcca
     2101 ggcgcgcggc cgcgagcagg accgcgaccg cgaacatcgc accgaccgcg ccggctcgtc
     2161 gccgcgcgtt tcggcctact tctccgaccg tcaccgcgag gtggtgcgtc agtattacgg
     2221 cgaccgcttc cgtgccggcg actgcccacc cggactggca aagaagggca acggctgcat
     2281 gccgccggga caggcgaaga agtggcgcat cggcgcgccg ctgccgcgcg acgtggtgtg
     2341 gtacgacgcg ccgggcgaca tcgtgctacg cctcggcatg ccgcccgaag gtcatcgctt
     2401 cgtccgcgtc gccgccgaca tcctgctgat cagtgtcggc accgggatgg tggtcgatgc
     2461 gatcgaggat ctcagccgcc tctgagcccc ggcgcggatc cgccacgagg cgggtccggt
     2521 gcctcgctgc gcgtgccacg cccacaaggc gaaccgatcg cgcatcgccc ggtccaccgc
     2581 ttgccgttcc tgccaggaga ccgatcatgt ccttcgcccg tcttgcccgt gccctcgccc
     2641 ccgccgttct gctcgccacc ctcgccccca acgccggcgc tgccgacgat gcgacgacgc
     2701 gcctctacga ccacagcctg cgccgtctgc acgccgacga gtcggtgcgg ctcgcagacc
     2761 gctacgccgg ccagcccgtg ctggtggtca acaccgccag ccactgcggc ttcacgcggc
     2821 agttccgcga gctcgaggcg gtgcaccagc gctacaggga gcgcgggctc aaggtcgccg
     2881 gctttccgtc cgacgacttc cgccaggagg ctgccgacga ggccgagacc gccgaggtct
     2941 gcttccagaa cttcggcgtc accttcgaca tgtacgcgcc gatccacgtc accggcaccg
     3001 aagcgcatcc cttgttccgc gagatcgcac gccagtcggc ggcgccggag tggaacttcc
     3061 acaagtacgt gatcgaccgc aacggccgcg tggtggcggc cttcggcagc cgcaccaagc
     3121 ccgacgcccc cgaggtgatc gcggcaatcg agaaggcgct gtgagcgccg cgccccacca
     3181 agcgcgacaa cgaggcggcc gccagccgcc cacggaaagg gagaacccat gaaggaatac
     3241 gacctgatcg ccatcggcgg cggcagcggt ggcgtcgcca ccgcccgccg cgccgcggag
     3301 tacggtgcgc gcgtgctgct gatcgaggcc gcccgcctcg gcggcacctg cgtcaacgtc
     3361 ggctgcgtgc ccaagaaggt gatgtggtac gcc
//
LOCUS       DAOQNN010000357         3377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_19305_length_3377_cov_7.092113, whole genome shotgun sequence.
ACCESSION   DAOQNN010000357 DAOQNN010000000
VERSION     DAOQNN010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19305_length_3377_cov_7.092113"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKV97_14480"
     CDS             complement(<1..300)
                     /locus_tag="PKV97_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNB07136.1"
                     /translation="MSPIDASRPHPPPRAGHVLVAALALGAGSAQAAGIDASGLAWNG
                     GLVLLGGGLGYLLGRRSLRRRTLAGAAEGRAADDPLHDDAPASGGAASRREWAGTP"
     gene            complement(429..1889)
                     /gene="glgA"
                     /locus_tag="PKV97_14485"
     CDS             complement(429..1889)
                     /gene="glgA"
                     /locus_tag="PKV97_14485"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNB07137.1"
                     /translation="MRILQVCAEIFPLLKTGGLADVAGALPPALGALGADVRVLLPGF
                     DAILAGLESPVEIARLQAPGGMGNNPARLLRGHLPAIGGEAYVIDAPGWYLRTGGPYA
                     DAAQQPYADNHLRFALLGWTAAELAQGLDTSWQPGVVHAHDWHAGLVPAWLQVAAARG
                     RSVPGCVFTVHNLAYQGMFPRACFDALGLPAHFFSIHGVEFHGHLNFMKAGLYYAGRV
                     TTVSPSYAREIQGPEQGCGLDGLLRARAHELSGILNGVDAAVWDPATDTLLPARYTAD
                     DMAGKARCRSALRRELGLAGKAEGPLYCVVSRLTEQKGLHLVLQALPALLAGGGQLAL
                     LGSGDAGLEGAFRDAAAAAPESVAVRIGYDEAFAHRLIAGSDVILVPSHYEPCGLTQL
                     YGLKYGTLPLVRRVGGLADTVADTRLETLDTDATGFVFEEFSAAALERTLLRVNAVHR
                     RPTEWRRVRARGMAKDFGWARAAREYVALYRGLTPA"
     gene            complement(1906..3174)
                     /gene="glgC"
                     /locus_tag="PKV97_14490"
     CDS             complement(1906..3174)
                     /gene="glgC"
                     /locus_tag="PKV97_14490"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNB07138.1"
                     /translation="MPAEAANRRRILTRRTLALVLAGGRGSRLRDLTNVRAKPAVHFG
                     GKFRIIDFALSNCMNSGLRRIGVITQYKSHSLLRHLQRGWSFLRNEMGEFVDLLPAQQ
                     RIDEEQWYQGTADAVFQNLDIIRNSTPPDYIVVLAGDHVYKMDYSIMLEDHAASGRGV
                     TVGCIEVPREEAKAFGVMAIDARRHITAFVEKPADPPALPGNPGLSLASMGIYIFSAN
                     YLYRLLEDDAKNPDSSHDFGKDLIPRAVAENQALAHPFTLSAIATPPFSGPYWRDVGT
                     VDAYWAANLDLASTTPALNMYDKDWPIWTYQEQLPPAKFVHDLDGRRGEALNALVSGG
                     CIVSGSVVRESVLFSNVLVRSYSTIEQAVVLPDVQINRHCRLKKVVIDRHCVIPERTV
                     IGEDAEADARRFHRTEGGVVLVTREMLDRL"
     gene            complement(3177..>3377)
                     /locus_tag="PKV97_14495"
     CDS             complement(3177..>3377)
                     /locus_tag="PKV97_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07139.1"
                     /translation="LLVNPAAASCRFRLPAGAWSPVFDSARADGSPALPAPGHESHPV
                     EPVLSARTVLVLLESAQHHQES"
BASE COUNT          489 a         1143 c         1260 g          485 t
ORIGIN      
        1 aggtgtgcct gcccactccc gcctcgaagc cgcgccgccg ctcgccgggg cgtcgtcgtg
       61 aagcgggtcg tccgcagcgc ggccctctgc cgcaccggcg agggtgcgtc ggcgcaggct
      121 gcggcggccg agcagatagc ccagcccgcc gccgagcaga accagaccgc cgttccaggc
      181 cagcccgctg gcatcgatcc ccgcggcctg cgccgagccc gcgccgaggg cgagcgccgc
      241 cacgagcaca tgcccggcac gaggcggtgg gtgcggtctg gacgcgtcga tggggctcac
      301 ctggtcagtg gcggcggtcc cgcatgccgc atggtttccg tggcggtcgg actcgtcctg
      361 tatagcacag aagctgcccg cggcgggccg tgcgcgaagc cggcgcggcg acgggcgccg
      421 gcgcggcctc atgcaggcgt gagaccacga taaagggcga catattcccg cgcggcacgc
      481 gcccagccga agtccttcgc catgccgcgg gcgcgcacgc gtctccattc ggtgggacgg
      541 cggtggacgg cattcacccg cagcagcgtg cgttcgagcg cagcggcgga gaattcctcg
      601 aagacgaagc cggtggcgtc ggtgtcgagc gtctccaggc gggtgtcggc taccgtgtcg
      661 gcgaggccgc cgacgcgccg caccagaggc agggtgccgt acttgaggcc gtagagctgg
      721 gtcaggccgc agggttcgta gtgcgagggc accaggatca cgtcgctgcc ggcgatcagg
      781 cggtgggcga aggcttcgtc gtagccgatg cgcaccgcca ccgattcagg cgcggctgcc
      841 gccgcgtcac ggaaggcgcc ctccagcccg gcgtcgccgc tgccgagcag ggcgagctgg
      901 ccgccgcccg ccagcagcgc gggcagggcc tgcagcacca ggtgcaggcc cttctgctcg
      961 gtgagccggc tcaccacgca gtacaacggg ccctcggcct tgcccgccag cccgagttcg
     1021 cggcgcagcg cgctgcggca gcgcgccttg ccggccatgt cgtccgcggt gtagcgcgcg
     1081 ggcagcaggg tatccgtggc cggatcccac accgccgcat cgaccccgtt gaggatgccc
     1141 gagagctcgt gcgcgcgggc gcgcaggagg ccgtccagcc cgcagccctg ttccggcccc
     1201 tggatctcgc gcgcatagct cgggctcacc gtggtgacgc gcccggcgta gtacagcccg
     1261 gccttcatga agttgaggtg cccgtggaac tccacgccgt ggatcgagaa gaaatgcgcg
     1321 ggcagcccga gcgcgtcgaa gcaggcgcgc gggaacatgc cctgataggc gaggttatgt
     1381 accgtgaaca cgcagcccgg cacgctccgc ccgcgcgctg ccgcgacctg caaccatgcc
     1441 ggcacgaggc cggcgtgcca gtcgtgcgcg tgcaccacgc cgggttgcca ggacgtgtcg
     1501 agcccttggg cgagctctgc tgcggtccac ccgagcaggg cgaagcgcag gtggttgtcg
     1561 gcgtagggct gctgtgcggc gtcggcgtag ggaccacccg tgcgcaagta ccagcccggg
     1621 gcgtcgatca cgtaggcctc cccgccgatc gcgggcaggt gtccgcgcag gagccgcgcc
     1681 gggttgttcc ccatcccgcc cggcgcttgc aggcgggcga tctcgaccgg cgactccagc
     1741 ccggcgagga tggcgtcgaa gccgggcagc agcacccgga cgtccgcgcc gagcgcgccc
     1801 agggcgggcg gcagcgcccc ggcgacgtcg gccagcccgc cggttttcag cagtgggaag
     1861 atctcggcac acacctgaag gatccgcatc gtctggtctc cgcgctcaca gtcggtcgag
     1921 catttcgcgc gtcaccagca ccacgccgcc ctcggtgcgg tggaagcggc gcgcatccgc
     1981 ctcggcgtcc tcgccgatca ccgtgcgctc ggggatcacg cagtggcgat cgatgacgac
     2041 cttcttcagg cggcagtggc ggttgatctg cacgtcgggc agcaccaccg cctgctcgat
     2101 cgtgctgtag gagcgcacca gcacgttgga gaacagcacc gactcgcgca cgaccgatcc
     2161 ggagacgatg cagccgcccg agaccagcgc gttgagcgcc tcgccgcggc gaccgtcgag
     2221 atcgtgcacg aacttggccg gcggtagttg ctcctggtag gtccagatcg gccagtcctt
     2281 gtcgtacatg ttgagcgccg gcgtggtcga ggcgaggtcg aggttggccg cccagtaggc
     2341 gtccaccgtg ccgacgtcgc gccagtaggg gccggagaag ggcggggtgg cgatcgccga
     2401 cagcgtgaag gggtgggcga gcgcctggtt ttccgccacc gcgcgcggaa tcaggtcctt
     2461 gccgaagtcg tggctggagt ccggattctt cgcgtcgtcc tcgagcaggc ggtagaggta
     2521 gttggccgag aatatgtaga tgcccatgct ggcgagcgac agcccgggat tgcccggcag
     2581 cgctggcggg tcggcgggct tctcgacgaa ggcggtgatg tggcgccgtg catcgatcgc
     2641 catcacgccg aaggccttgg cctcctcgcg cggcacctcg atgcagccca cggtgacacc
     2701 gcgcccgctc gcggcgtggt cctcgagcat gatcgagtag tccatcttgt acacatggtc
     2761 gccggcgagc acgacgatgt agtcgggcgg cgtggagttg cggatgatgt cgaggttctg
     2821 gaacaccgcg tcggcggtgc cctggtacca ctgctcttcg tcgatgcgct gctgcgccgg
     2881 cagcaggtcg acgaattcgc ccatctcgtt gcgcaggaag ctccagccgc gctgcaggtg
     2941 gcgcagcagc gagtgcgact tgtactgtgt gatcacgccg atgcggcgca ggcccgagtt
     3001 catgcagttc gacagcgcga agtcgatgat gcggaacttg ccgccgaagt gcaccgccgg
     3061 cttggcgcgc acgttggtga gatcgcgcag gcgcgagccg cgcccgccgg cgagcacgag
     3121 ggcgagggtg cggcgcgtga ggatccgtcg tcggttggcg gcttcggcag gcatgatcag
     3181 gactcctggt ggtgttgtgc ggattctagg aggacgagca cggtgcgcgc ggacagcacc
     3241 ggctcgacag gatggctttc gtggccgggg gcgggcagcg ccgggctgcc gtcggcccgg
     3301 gcactgtcga agaccggcga ccatgcgccc gcgggcaggc ggaagcggca cgaggccgcg
     3361 gcgggattga cgagcag
//
LOCUS       DAOQNN010000358         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_19310_length_3376_cov_12.872930, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000358 DAOQNN010000000
VERSION     DAOQNN010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19310_length_3376_cov_12.872930"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /gene="rplL"
                     /locus_tag="PKV97_14500"
     CDS             complement(<1..226)
                     /gene="rplL"
                     /locus_tag="PKV97_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987814.1"
                     /note="present in two forms; L12 is normal, while L7 is
                     aminoacylated at the N-terminal serine; the only multicopy
                     ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two
                     L12 dimers bind L10; critically important for translation
                     efficiency and fidelity; stimulates GTPase activity of
                     translation factors; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNB07140.1"
                     /translation="MAISKEDILEAVAGMTVMELNDLVKAFEEKFGVSAASLAVAAPG
                     AGAAAAPVEEKTEFDVVLLEAGAKKVEVIKV"
     gene            complement(284..787)
                     /gene="rplJ"
                     /locus_tag="PKV97_14505"
     CDS             complement(284..787)
                     /gene="rplJ"
                     /locus_tag="PKV97_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNB07141.1"
                     /translation="MGLNLDDKKAVVAEVSAQVANAQTIAVAEYRGIAVGDLTALRAK
                     ARESGVYLRVLKNTLVRRAIAGTPFEGLAGDLTGPLIYGISADPVAAAKVLNDFAKGN
                     DKIVLRAGSYAGKTLDKAAVQALASIPSREELLAKLLGVMQAPVSGFAGALAALAKKR
                     EEEGAAA"
     gene            complement(1039..1737)
                     /gene="rplA"
                     /locus_tag="PKV97_14510"
     CDS             complement(1039..1737)
                     /gene="rplA"
                     /locus_tag="PKV97_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNB07142.1"
                     /translation="MAKLSKRVQALRAKVDRNQAYPVADALRLVKECATAKFDESIDI
                     AVNLGVDARKSDQVVRGSVVLPAGTGKSVRVAVFAQGDKAEAARAAGAEVVGFDDLAE
                     QVKAGNLDFDVCIATPDAMRVVGQLGQILGPRGLMPNPKVGTVTMDVATAVKNAKAGQ
                     VQYRTDKAGLVHATIGRASFEVDALQQNLTAFVDALVKAKPAAAKGIYLRRIAVSSTM
                     GAGVRVEPGSLNPA"
     gene            complement(1740..2171)
                     /gene="rplK"
                     /locus_tag="PKV97_14515"
     CDS             complement(1740..2171)
                     /gene="rplK"
                     /locus_tag="PKV97_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNB07143.1"
                     /translation="MAKKITGYIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     KTQGMEPGLPIPVVITAFADKSFTFIMKTPPATILIKKAAGIQKGSAKPHTDKVGKIT
                     RAQVEDIAKAKQPDLTAADLEAAVRTIAGSARSMGITVEGL"
     gene            complement(2289..2822)
                     /gene="nusG"
                     /locus_tag="PKV97_14520"
     CDS             complement(2289..2822)
                     /gene="nusG"
                     /locus_tag="PKV97_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNB07144.1"
                     /translation="MSKRWYVVHAYSGFEKSVQRALIDRVNRSGMQDSFGQILVPVEE
                     VIEMRGGQKTVTERKFFPGYVLVEMEMNDESWHLVKSTPKVTGFVGGTANKPAPISEK
                     EVEKIMQQMQEGVDKPRPKVLFEIGEVVRVKEGPFTDFHGSVEDANYEKNKLRVSVTI
                     FGRATPVELDFAQVEKT"
     gene            complement(2824..3171)
                     /gene="secE"
                     /locus_tag="PKV97_14525"
     CDS             complement(2824..3171)
                     /gene="secE"
                     /locus_tag="PKV97_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNB07145.1"
                     /translation="MADKLKFALALALLAAGVVGFYVLSEQPLVLRVLSVLAGLGAGI
                     AVAALSEPGRRFIGFARESITETKKVVWPSRKETTQTTGLVFAFAVVMALFLWLTDKS
                     LEWVLYDLVLGWK"
     gene            complement(3196..3271)
                     /locus_tag="PKV97_14530"
     tRNA            complement(3196..3271)
                     /locus_tag="PKV97_14530"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3236..3238),aa:Trp,seq:cca)
BASE COUNT          587 a         1107 c         1067 g          615 t
ORIGIN      
        1 cgaccttgat gacctcgacc ttcttggcgc cggcttcgag cagcacgacg tcgaattcgg
       61 tcttctcttc caccggggcg gcggcggcac cggcacccgg agcggccacg gccagcgagg
      121 ccgcggacac gccgaacttc tcttcgaacg ccttgaccaa gtcgttcagt tccatcacgg
      181 tcatgccggc gacggcttcc aggatgtctt ctttgctgat tgccatttca aatcactcca
      241 gaatcgataa tcttgcgaaa attttcgtag cggaaggctg cggtcaggcg gcagcgcctt
      301 cttcctcgcg cttcttggcg agggcggcga gcgcaccggc aaaaccggac accggcgcct
      361 gcatgacgcc cagcagcttg gcgagcagct cttcgcggct cgggatcgaa gcgagcgcct
      421 gcacggcggc cttgtcgagc gtcttgcccg cgtaggaacc ggcgcgcagc acgatcttgt
      481 cgttgccctt ggcgaagtcg ttcagcacct tggctgcagc gaccggatcg gccgaaatgc
      541 catagatcag cggaccggtc agatcgcccg ccagcccctc gaacggggtg ccggcgatcg
      601 cacgacggac cagggtgttc ttcagcacgc gcaggtacac gccagactcg cgggccttgg
      661 cacgcagcgc ggtgaggtcg cccaccgcaa tgccgcgata ctcggccacc gcgatcgtct
      721 gggcattggc cacttgtgcc gacacctcag ccactacggc tttcttgtca tcgagattga
      781 gacccacagg tctttcctcc tttcaagtca acacgcgaac cgctcgcgcg gctcgcacct
      841 acggcgacct ggatcaggag cgcggccggc gaaccggcca aatcctgttc tgagcacacc
      901 gtctacgcag gccattcaca tgcttaagcg gcaccttgcc ggggccacac ctgcggtctt
      961 tgacgatccg cagccccgga cgggcggcta cggctcaaag ttcggcggag cgcccgccct
     1021 gggcggacgt cctggcaatc aggcagggtt gaggctgccc ggctccacgc gcacgccggc
     1081 acccatggtg ctggagacgg cgatgcggcg caggtagatc cccttcgccg cggccggctt
     1141 ggccttgacg agggcatcga cgaaggcggt caagttctgc tgcagcgcat cgacctcgaa
     1201 cgaggcgcgg ccgatggtgg cgtgcaccag gcccgccttg tcggtgcggt actgcacctg
     1261 gccggccttg gcgttcttca ccgcggtggc gacgtccatg gtcacggtgc cgaccttggg
     1321 gttcggcatg aggccgcgcg gaccgagaat ctggccgagc tggccgacca cgcgcatggc
     1381 gtcaggggtc gcgatgcaga cgtcgaaatc gagattgccg gccttgacct gctcggcgag
     1441 gtcgtcgaag ccgacgacct cggcaccggc ggcgcgggcg gcctcggcct tgtcgccctg
     1501 ggcgaacacg gcgacgcgaa ccgacttgcc ggtacccgcg ggcagcacga ccgaaccgcg
     1561 tacgacctgg tcggacttgc gcgcatcgac accaaggttg accgcgatgt cgatcgactc
     1621 gtcgaacttg gcagtggcgc actccttgac cagacgcagg gcgtcggcaa ccgggtaggc
     1681 ctggttacgg tcgaccttgg cgcgcagcgc ctgaactcgc ttggacagct tcgccatgat
     1741 tacaggccct ccacggtgat gcccatgctg cgggcggaac cggcgatggt gcgcacggcg
     1801 gcttcgaggt cggcagcggt gaggtccggc tgcttggcct tggcgatgtc ctcgacctgg
     1861 gcacgagtga tcttcccgac cttgtcggtg tgcggcttgg ccgaaccctt ctggatcccg
     1921 gcggccttct tgatcaggat cgtggccggc ggagtcttca tgatgaacgt gaaggacttg
     1981 tcggcgaagg cggtgatcac caccggaatc ggcagaccgg gctccatgcc ctgggtcttg
     2041 gcgttgaacg ccttgcagaa ttccatgatg ttcaggccgc gctgaccgag cgccggaccg
     2101 atcggagggc tggggttggc cttgccggcc ggaacctgca gcttgatgta gccagtgatt
     2161 ttctttgcca tctaggcttg ctcctgtgat gagtgctagc gcaccggaga acccggcgct
     2221 cctcgcagtg cccgcgctcg tcggccgagt gcacgggctt cggcaggcct cgcggcctgc
     2281 cgtcttgctc aggtcttctc gacctgggcg aaatcgagtt ccaccggcgt ggcgcgaccg
     2341 aagatggtca ccgacacgcg cagcttgttc ttttcgtagt ttgcatcctc gacgctgcca
     2401 tggaagtcgg tgaacggccc ttccttgacg cgaaccacct cgccgatctc gaacagcacc
     2461 ttgggacgcg gcttgtcgac gccttcctgc atctgctgca tgatcttctc gacctccttt
     2521 tcggagatcg gggcgggctt gttcgccgta ccgccgacga agccggtgac cttgggcgtc
     2581 gacttcacca agtgccagga ctcgtcgttc atttccattt cgacgagaac atagccgggg
     2641 aagaacttgc gctcggtgac ggtcttctgg ccgccgcgca tctcgatgac ctcttcgacg
     2701 ggcaccagga tctgaccgaa actatcctgc atgcccgatc gattgacgcg gtcgatcagc
     2761 gcgcgctgaa cggacttttc gaagcccgaa taggcgtgaa ccacatacca acgcttgctc
     2821 atatcacttc caacccagga ccagatcgta caggacccac tccaggctct tgtccgtcag
     2881 ccacaggaag agcgccatca ccaccgcgaa cgcgaacacc agaccagtgg tctgcgtggt
     2941 ttccttgcgg ctcggccaga caaccttctt ggtttcggtg atcgattcgc gggcaaaacc
     3001 gatgaagcgg cgacccggct cggagagggc ggcgacggcg atgccggcac ccaggcccgc
     3061 aagcacggaa agcacgcgca gcacgagagg ctgctcggac agcacgtaga agccgactac
     3121 gccggctgcc aacagtgcca gcgccagcgc aaatttcaac ttatcggcca ttgcggatga
     3181 acacccggaa aatgttggca ggggcagagg gaatcgaacc cccaaccttc ggttttggag
     3241 accgacgctc tgccagttga gctatacccc tgcagcggac actgcaaggc gcacccgagc
     3301 gggtgcgcct gtctggcaat aatcgagcga ttactcgatg atcttggcga cgacgccggc
     3361 gccgacggta cggcca
//
LOCUS       DAOQNN010000359         3367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_19406_length_3367_cov_10.458031, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000359 DAOQNN010000000
VERSION     DAOQNN010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19406_length_3367_cov_10.458031"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKV97_14535"
     CDS             complement(<1..231)
                     /locus_tag="PKV97_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide succinyltransferase"
                     /protein_id="HNB07146.1"
                     /translation="MLIEVKVPQLSESVSEATLVTWHKKEGDAVARDENLIDIETDKV
                     VLETPAPADGVLVKIIKQGGDTVTSGELIAQID"
     gene            complement(255..3104)
                     /locus_tag="PKV97_14540"
     CDS             complement(255..3104)
                     /locus_tag="PKV97_14540"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNB07147.1"
                     /translation="MMKQLEQTSHLFGSNAPFIEEQYENYLADPASVSAEWREYFDKL
                     QAQVGAAARDVPHGPVIAAFEQMAKRGPVRTIVTAGEDKQQVSVLQLINAYRFLGNRW
                     ANLDPLKRTERPQIAELEPSYYGFTEADLSKSFNVGSFHGFSTERATLREILEALRQT
                     YCGSIGAEYMYMTDIGQKRWIQSRLESLRGTPKFSAEMKKRILERTTAAETLERYLHT
                     RYVGQKRFSLEGGESAIVAMDELIRVAGSLGVQESVIGMAHRGRLNVLVNTLGKSPSM
                     LFSEFEGKAAADLTAGDVKYHMGFSSDVMTPGGPMHLTLAFNPSHLEIINPVVEGSVY
                     ARQVRRGDADKKQVLPVLIHGDAAVAGQGVNQEMLNFSQTRGYGTGGTVHLVVNNQIG
                     FTTSDPRDYRSSLYCTDIFKMVEAPIFHVNGDDPEAVALVTALAVEFRQEFKKDVVVD
                     IICFRKLGHNEQDEPMVTQPLMYRTIQKHPGTRKLYAERLVAEGTLKAEEPDQMIAEY
                     REHLDKGQLLYNPVLSGHNRQFAADWTPYIKQPYTDECDTTVPVQEIKRLSERLSAIP
                     ANFTLHSRVKKIIDDRAAMGRGEAPFDWGMGENLAYASLLAQGYGVRLSGEDVGRGTF
                     FHRHAVLHDQKRERWDEGIYKPLDHIQDGQARFQSYDSVLSEEGVLAFEYGYATTEPN
                     ELVIWEAQFGDFVNGAQVVLDQFISSGEAKWGRLCGLTLMLPHGYEGQGPEHSSARLE
                     RYMNNAAEHNWQICVPTTPAQIFHLLRRQAIRKVRKPLIIITPKSLLRHKEAISSIEE
                     LANGSFQTVIPEVEKLDAKKVKRVVLCQGKIYYELLAHRRENKITDTALVRIEQLYPF
                     PTEAFAKAIEQFPNAKEIVWAQEEPRNQGAWYWLASRQHLVNVLGTKRRLLLVSRPAA
                     ASPAVGYYAKHNAQQKAVLENAFGPIQDTTPQSPH"
BASE COUNT          500 a         1032 c         1176 g          659 t
ORIGIN      
        1 gtcaatctgg gcgatcagct cacccgaggt cacggtgtcg ccaccctgct tgatgatctt
       61 caccagcaca ccgtcggccg gggccggggt ctcgagcacg accttgtcgg tctcgatgtc
      121 gatcaggttc tcgtcgcgcg cgaccgcgtc gccttccttc ttgtgccagg tgaccagcgt
      181 ggcttcggaa accgactcgg acagctgcgg tactttgact tcgatcagca tggatttctc
      241 tccctaggta tttttcagtg cggggactgc ggcgtggtgt cctggatcgg accgaaggcg
      301 ttttcgagga ccgccttttg ttgcgcgttg tgcttggcgt agtagccgac cgcgggcgag
      361 gcggcggccg ggcggctcac cagcagcaga cggcgcttgg tgccgagcac gttgaccagg
      421 tgctggcgcg aggcgagcca gtaccacgca ccctggttgc gcggctcttc ctgggcccag
      481 acgatctcct tggcgttcgg gaactgctcg atcgccttgg cgaaggcctc ggtcgggaac
      541 ggatagagct gctcgatgcg gaccagcgcg gtgtcggtga tcttgttctc gcggcgatgg
      601 gcgaggagct cgtagtagat cttgccctgg cacagaacca cgcgcttgac cttcttggcg
      661 tcgagcttct cgacctcggg gatcacggtc tggaagctgc cgttggccag ctcctcgatc
      721 gaggagatcg cctccttgtg acgcagcagc gacttcggcg tgatgatgat cagcggcttg
      781 cgcaccttgc ggatcgcctg gcggcgcagc aggtggaaga tctgcgccgg ggtggtcggc
      841 acgcagatct gccagttgtg ctcggcggcg ttgttcatgt agcgctcgag acgcgccgag
      901 gagtgctcgg ggccttggcc ctcgtagccg tgcggcagca tcagcgtgag tccgcacagg
      961 cgaccccact tggcctcgcc cgagctgatg aactggtcga gcaccacctg ggcgccgttg
     1021 acgaagtcgc cgaactgggc ctcccagatc acgagctcgt tgggctcggt cgtggcgtag
     1081 ccgtattcga aggcgagcac gccttcctcc gacaacaccg agtcgtagct ctggaagcgg
     1141 gcctggccgt cctggatgtg gtcgagcggc ttgtagatgc cttcgtccca gcgctcgcgc
     1201 ttctggtcgt gcagcacggc gtggcgatgg aagaaggtgc cgcggccgac gtcctcgccg
     1261 gagaggcgca cgccgtagcc ctgcgccagc aggctggcgt aggccaggtt ctcgcccatg
     1321 ccccagtcga agggcgcttc gccacgcccc atcgcggcgc ggtcgtcgat gatcttcttg
     1381 acgcgcgagt gcagcgtgaa gttcgccggg atggcactca ggcgctcgga caggcgcttg
     1441 atctcctgca ccggcaccgt ggtgtcgcac tcgtcggtgt agggctgctt gatgtagggc
     1501 gtccagtccg cggcgaactg gcggttgtgg ccggagagca ccgggttgta gagcagctgg
     1561 cccttgtcga gatgctcgcg gtactcggcg atcatctggt cgggctcctc ggccttcagg
     1621 gtgccctcgg cgaccaggcg ctcggcgtag agcttgcggg tgccggggtg cttctggatg
     1681 gtgcggtaca tcagcggctg cgtcaccatc ggctcgtcct gctcgttgtg gccgagcttg
     1741 cggaagcaga tgatgtcgac cacgacgtcc ttcttgaact cctggcggaa ttcgaccgcc
     1801 agcgcggtga ccagggccac ggcctcgggg tcgtcgccat tgacgtggaa gatcggcgcc
     1861 tcgaccatct tgaagatgtc ggtgcaatac agcgaggagc ggtagtcgcg cgggtcggag
     1921 gtggtgaagc cgatctggtt gttgacgacc aggtgcaccg tgccgcccgt gccgtagccg
     1981 cgagtctgcg agaagttgag catttcctgg ttaacgccct ggccggccac ggcggcgtcg
     2041 ccgtggatca gcaccggcag cacctgcttc ttgtcggcgt cgccgcgacg gacctggcgg
     2101 gcatacaccg agccctcgac caccgggttg atgatctcga ggtgcgaggg gttgaacgcg
     2161 agcgtcaggt gcatcgggcc gcccggggtc atcacgtcgc tcgagaagcc catgtggtac
     2221 ttcacgtcgc cggcggtgag gtcggccgcg gccttgccct cgaactccga gaacagcatc
     2281 gagggcgact tgcccagggt gttgacgagc acgttgaggc ggccgcggtg ggccatgccg
     2341 atcacgcttt cctgcacacc caggctgccg gcgacgcgga tcagttcgtc catcgcgacg
     2401 atggccgact cgccgccttc gagcgagaag cgcttctggc caacgtagcg ggtgtgcaga
     2461 tagcgctcca gggtctcggc cgcggtggtg cgctcgagga tgcgcttctt catctccgcc
     2521 gagaacttcg gcgtgccgcg caggctctcg aggcggcttt ggatccagcg cttctgaccg
     2581 atgtcggtca tgtacatgta ctcggcgccg atcgagccgc agtaggtctg gcgcagcgcc
     2641 tcgaggatct cgcgcagggt ggcgcgctcg gtgctgaagc cgtggaagga gccgacgttg
     2701 aagctcttgg agagatcggc ttcggtgaag ccgtaatagg acggctcgag ctcggcgatc
     2761 tgcgggcgct cggtgcgctt gagcggatcg aggttggccc agcggttgcc gaggaagcgg
     2821 taggcgttga tcagttgcag cacggagacc tgctgcttgt cctcgccggc ggtgacgatg
     2881 gtgcgcaccg ggccgcgctt ggccatctgc tcgaaggcgg caatgaccgg accatgcggc
     2941 acgtcgcgcg cggcggcgcc gacctgagcc tgcagcttgt cgaaatattc gcgccactcg
     3001 gcggacaccg aggccggatc ggcgaggtag ttttcgtact gttcttcgat gaacggcgcg
     3061 ttcgagccga acaagtgcga agtctgttcg agttgcttca tcatgggata agcacctgtg
     3121 gtgttctgtt gccctgtttt cgggtgcggg agtgcagccg acgcgtcgat gccgatggaa
     3181 aaacgggatg gccgcgcacg cgcaaccacc ccgtcaccgt cagtcgcggc ggatcctgca
     3241 acccctcttc atcgtctctt acggacgctg cgcgagcgcc ggcacgtcac gacgcgcggt
     3301 gccgacgtag agctggcgcg gacggccgat cttgtattcc ggatcggtga tcatctcttc
     3361 ccactgc
//
LOCUS       DAOQNN010000360         3361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_19482_length_3361_cov_5.534483, whole genome shotgun sequence.
ACCESSION   DAOQNN010000360 DAOQNN010000000
VERSION     DAOQNN010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19482_length_3361_cov_5.534483"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2573
                     /gene="kdpD"
                     /locus_tag="PKV97_14545"
     CDS             <1..2573
                     /gene="kdpD"
                     /locus_tag="PKV97_14545"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HNB07148.1"
                     /translation="YAMLGAARDARANGVDVVVGLAETHGRAETEALLAGLEVLARRR
                     VDYRERTLAEFDLDAALARRPALLLVDELAHSNAPGSRHPKRWQDVEELLAAGIDVWT
                     TLNVQHLESLNDVVGGITGVRVWETVADRVFDAADEVALVDLPPDDLLTRLKEGKVYL
                     PAQAAMAVENFFRRANLIALRDLSLRRLAERVDAQVQTARAAAPGARVWATAESLLVC
                     LGPGDTDGKLIRSAARLASKLDARWHAVYIETPRLQRLPEDRRSAILATLRLAQEMGA
                     ETAVLAEPDLVDAALDYARAHNLGKIVIGRRARPQRRWWGQFMRRLGERAPDIDLIAV
                     ARRAEWPPAPRQTAPPPSAPAGRVAPYAGAAALCVAVALLATPLRYALELSNIVMLFL
                     LAVVGAAYRWGRGPAVLAAVLSVALFDFFFVPPRFTFAVSDMQYLVTFAVMLIVGLTI
                     GQSMVAVRYQARIAGHREQRIQSLYRMARDLSAALGEAQVAEIGARSLVATFHAQVRV
                     LLPDTQGKLAAPVEAMPQVDLAIAQWCFDHGEPAGIGTDTLPAAAAAYLPLKAPAGMQ
                     GVLAVQPDSRRLLHIPEQRRLLETHATLIAMALERLHFVTQVHETRLGAASERLRSSL
                     LAALSHDLRTPLTALVGLADTLTLRLLAAQAPESETAATIRDQALRTGHMVDNLLEMA
                     RLQSGTVTPRRDWLVLEEVVGAAVRALEPALAEHPLQTDLPADLPLVQGDAVMLERVL
                     VNLLDNAIKYTPPGTPIGITAAVAGETLEVAVWDEGPGLPPGQEQVIFERFARGTHES
                     AITGVGLGLAICQAIVAAHGGHIRAENRPAGGARFVFTLPLAPPPAPPAETAPSTPPH
                     "
     gene            2606..3253
                     /gene="kdpE"
                     /locus_tag="PKV97_14550"
     CDS             2606..3253
                     /gene="kdpE"
                     /locus_tag="PKV97_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HNB07149.1"
                     /translation="MRRTLEDAGLKVAEAATLARGLIEAGTRRPDLVILDLGLPDGDG
                     RDFITALRAWSRVPVIVLSARIEEADKVAALDAGADDYLTKPFGVAELLARVRVALRH
                     GRSGVEAGSVVHLGPVTVDLVARRVQRGGADIHLTAVEFRLLTVLLKAPGRVLTHRHL
                     LREVWGPNSSEHSHYLRIYMARLRHKLEADPARPRYLVTETGVGYRCVPDLPAAG"
BASE COUNT          457 a         1228 c         1199 g          477 t
ORIGIN      
        1 cctacgccat gctcggcgcc gcccgcgacg cgcgggccaa cggcgtggac gtggtggtcg
       61 gcctcgccga aacccacggc cgggccgaga ccgaagccct gctcgcgggc ctcgaggtgc
      121 tggcgcgccg gcgcgtcgac taccgcgagc gcacgctcgc cgagttcgac ctcgacgccg
      181 cgctcgcgcg ccggccggcg ctgctgctgg tggatgagct cgcgcacagc aacgcgccgg
      241 gcagccggca tccgaagcgc tggcaggacg tggaggaact gctcgccgcc ggcatcgacg
      301 tatggaccac gctcaacgtc cagcatctcg aaagcctgaa cgacgtggtc ggcggcatca
      361 ccggtgtgcg cgtgtgggaa accgtcgccg accgggtgtt cgacgccgcc gacgaggtgg
      421 cgctggtcga cctgccgccg gacgatctgc tcacgcgtct gaaggaaggc aaggtctacc
      481 tgccggcaca ggccgcgatg gctgtggaga atttcttccg tcgtgccaac ctcatcgcgc
      541 tgcgcgacct gtcgctgcgg cgcctggccg agcgcgtgga cgcgcaggtg cagacggcgc
      601 gcgcggcggc gcccggcgcg cgggtatggg ccaccgccga gtcgctgctg gtgtgtctcg
      661 gacccggcga caccgacggc aagctcatcc gcagcgcggc gcgtctcgca agcaagctcg
      721 acgcgcgctg gcacgccgtc tacatcgaaa cgccgcgtct gcagcgcctg ccggaagacc
      781 ggcgcagcgc catcctcgcc acgctgcgcc tggcccagga gatgggcgcc gaaaccgcgg
      841 tgctggcgga gcccgatctg gtcgatgcgg cgctcgacta cgcccgcgcc cacaacctcg
      901 gcaagatcgt gatcggccgg cgcgcccgcc cgcagcggcg ctggtggggc cagttcatgc
      961 ggcggctcgg cgagcgggcg ccggatatcg atctcattgc cgtggcgcgg cgggcggaat
     1021 ggccgcccgc gccgcgccag acggcaccgc cacccagcgc tccggccggg cgcgttgcac
     1081 cctacgccgg ggctgccgcg ttatgcgtgg cggtagcgct gctggcgacg ccgctgcggt
     1141 acgcactgga actcagcaac atcgtgatgc tgttcctgct cgccgtggtg ggcgcggcct
     1201 accgctgggg acgcgggccc gccgtgctgg cggcggtgct gtcggtggcg ctgttcgatt
     1261 tcttcttcgt gccgccgcgg ttcacgtttg cggtcagcga catgcaatac ctcgtcacct
     1321 tcgcggtcat gctcatcgtc ggactcacca tcggccagtc gatggtggcc gtgcgctacc
     1381 aggcgcgcat cgccggccac cgcgagcagc gcatccagag cctctaccgg atggcgcgcg
     1441 acctgtcggc ggcgctcggc gaggcgcagg tggccgagat cggcgcgcgg tcgctggtgg
     1501 cgacgtttca tgcccaggtc cgggtcctgc tgcccgacac gcagggcaag ctcgccgcac
     1561 ccgtcgaggc aatgccgcag gtcgatctcg ccatcgcgca gtggtgtttc gatcatggcg
     1621 agccggccgg catcggcacc gacacgctgc cggccgctgc cgccgcctac ttgccgctga
     1681 aggcgccggc cggcatgcag ggtgtgctcg cggtgcagcc ggacagccgc cgcctgctgc
     1741 acatccccga acagcgccgc ctgctcgaaa cccatgccac cctgatcgcc atggcgctcg
     1801 agcgcctgca cttcgtcacc caggtgcacg agacgcgcct gggcgccgcc tccgagcgcc
     1861 tgcgcagttc actgctggcg gcgctgtcgc acgatttgcg cacgccactg accgcgctgg
     1921 tgggcctcgc cgacacgctg acgctgcgcc tgctcgccgc ccaggcgccg gaaagcgaga
     1981 ccgccgccac gattcgcgac caggcgctgc gcaccggcca catggtggac aacctgctcg
     2041 agatggcgcg cctgcagtcc ggcaccgtca ccccgcgccg cgactggctg gtgctggagg
     2101 aggtggtcgg tgcggcggtg cgggcgctcg aacccgcgct cgccgagcat ccgctgcaga
     2161 ccgatctgcc ggccgacctg ccgctggtgc agggcgacgc cgtcatgctg gaacgggtgc
     2221 tggtcaacct gctcgacaac gccatcaagt acacgccgcc cggcacgccg atcggcatca
     2281 ccgcggcggt ggcgggcgag acgctggagg tcgccgtgtg ggacgaagga cccggcttgc
     2341 cgccgggtca ggagcaggtg atcttcgagc gcttcgcgcg cggcacgcac gagtcggcca
     2401 tcaccggcgt cgggctcggg ctcgccatct gccaggccat cgtcgccgca catggcgggc
     2461 acatccgcgc cgaaaatcgc cccgcgggcg gggcgcgctt cgtcttcacc ctgccgctcg
     2521 cgccgccgcc ggcgccgccc gcggagaccg cgccatcgac gccgccgcac tgatcgtcga
     2581 ggacgatgcg gcgatccgcc tgttcgtgcg ccgcacgctg gaagacgcgg gcctcaaggt
     2641 agcggaagcc gccacgctgg cgcgcgggct aatcgaagcc ggcacgcggc ggccggatct
     2701 cgtgatcctc gaccttggcc tgcccgatgg tgacggccgc gacttcatca ccgcgctgcg
     2761 cgcctggagc cgggtgccgg tgatcgtgct gtcggcgcgc atcgaggaag ccgacaaggt
     2821 ggcggcgctc gacgccggcg cggacgacta cctcaccaaa cccttcggcg tcgcggaact
     2881 gctggcgcgg gtgcgcgtgg cgctgcgcca cggccgcagc ggcgtcgaag ccggctcggt
     2941 ggtgcacctc gggccggtga cggtggatct ggtggcgcgg cgcgtgcagc gcggcggcgc
     3001 ggacatccac ctcacggcgg tcgagttccg cctgctgacc gtactgctga aggcgccggg
     3061 ccgcgtgctg acccatcgcc acctgctgcg cgaggtatgg ggcccgaaca gcagcgagca
     3121 cagccactac ctgcgcatct acatggcgcg cctgcgccac aagctcgagg ccgacccggc
     3181 ccggccacgg tatctcgtga ccgagaccgg ggtaggctat cgctgtgtgc ccgatcttcc
     3241 tgcagcgggg tgaatgtcac gacagggtaa ctgagtcacc caccgcagcg agctgcaggg
     3301 gctgctggtc tgcggcaccc tgattgctac cttccccgaa catctgccgc tgccggcgtg
     3361 g
//
LOCUS       DAOQNN010000361         3352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_19576_length_3352_cov_7.947225, whole genome shotgun sequence.
ACCESSION   DAOQNN010000361 DAOQNN010000000
VERSION     DAOQNN010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19576_length_3352_cov_7.947225"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1274
                     /locus_tag="PKV97_14555"
     CDS             126..1274
                     /locus_tag="PKV97_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB07150.1"
                     /translation="MKTLRRLFVLALVAALVGAGIWWMTRPQPIEVAVHEVARGTVEA
                     TLTNTRAGEVEACQRAKMATIAGGRIEWLGVEEGDRVEAGQVLMRLWHGDLDARAAVV
                     RAQLATARQRVQEACAVAANAEREAARQAQLVKRGFVSASAEERARTEARARRAACDT
                     AQADIATAQAQLVATETERQRLVLVAPFAGTVAKISGELGEYATPSPPGVPMPPAIDL
                     IDDSCLYVKAPMDEIDAPKIRPGLPVRITLEAIPGKVFEGRVRRVAPYITAVEKQART
                     VAVDVDFADPAEAHGMLVGYSTDVEIVLATRAEVLRVPTGALREGGKVLVVEGDTLVE
                     RTLRTGVANWEFTEVVSGLAAGERIVTSLEREGVMAGAKVVLRPEEAR"
     gene            1609..2304
                     /locus_tag="PKV97_14560"
     CDS             1609..2304
                     /locus_tag="PKV97_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB07151.1"
                     /translation="MAQIELEHIDRIFKLGDSEVHALRDITLRIEPGEYVAVMGPSGS
                     GKSTLLNLLGLLDRPDAGHYRLEGRDVTTLSADEQAAVRSARIGFVFQSFHLVPRLSA
                     AENIGLPLVLAGIPVAERAARIAQALQDFGLEGRARHRPDELSGGQRQRVAIARATIM
                     RPAMLLADEPTGNLDRATGHEVTALLEALNAAGTTLIVVTHDPSMGERAHRRILMEDG
                     AIRQDLRTAAPPP"
     gene            2301..>3352
                     /locus_tag="PKV97_14565"
     CDS             2301..>3352
                     /locus_tag="PKV97_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB07152.1"
                     /translation="MSPADTLRFATRAATAWPLRTALMMLAMSIGVAAVVVLTALGDG
                     ARRYVVNEFSALGASLLIVLPGRTETGGVNAGSFVSSTPRELTNADAAALLRLPLVQR
                     VAPLAVGNSEIAVGGRLREVTVVGTSADFLELRGLKIGHGGFLPREDFNRASAVAVIG
                     DALRSELFPGQSAVGRMIRVGDTRLRVIGVLTPSGRGLGMTTDELVLVPVATAQAMFD
                     TSGLFRIFVEARGREALPATQRQIEERLRARRDDELDFTVITQDAVLGTFDRILGALT
                     LGVAGIAAISLAVAGILVMNVMLVAVTQRTAEIGLLKALGARAGTIRAAFLAEAALLS
                     VAGALAGFALGHAGAWG"
BASE COUNT          468 a         1230 c         1196 g          458 t
ORIGIN      
        1 cgaagcccgg cgcgccggtg ttcaaccgca cggtgacgct gaaggattcg tgggccaagg
       61 gcgggaagtg agatcgcatc cttcgcgcgc ggcgaccaga ccttgaccgc tccggcactc
      121 cactcatgaa gaccctccgc cgcctcttcg ttctcgcact cgtcgcggcg ctggtgggcg
      181 cggggatctg gtggatgacc cgcccgcagc cgatcgaggt ggcggtgcac gaggtggccc
      241 gcggtacggt cgaggccacg ctgaccaaca cccgcgccgg cgaggtcgag gcctgccagc
      301 gcgcgaagat ggcgacgatc gcgggcgggc ggatcgagtg gctgggcgtg gaggaaggcg
      361 accgcgtcga ggccggacag gtgctgatgc ggctgtggca tggtgacctc gacgcgcgcg
      421 cggcggtcgt gcgcgcccag ctcgccaccg cccgccagcg cgtgcaggag gcgtgcgccg
      481 tggcggccaa cgccgagcgc gaggccgcgc gccaggcgca gctggtgaag cgcggcttcg
      541 tgtccgccag cgccgaggag agggcccgca ccgaggcgcg tgcccgccgc gccgcctgcg
      601 acaccgcgca ggccgacatc gccaccgccc aggcccagct cgtcgcgacc gaaaccgagc
      661 gccagcgcct ggtgttggtt gcgcccttcg ccggcaccgt ggccaagatc agcggcgagc
      721 tgggcgaata cgcgacgccc tccccgcccg gcgtgccgat gccgccggcg atcgacctga
      781 tcgacgactc ctgcctgtac gtgaaggcgc cgatggacga aatcgacgca ccgaagatcc
      841 ggcccgggct gccggtacgc atcacgctcg aggccatccc cggcaaggtc ttcgaaggcc
      901 gcgtgcgccg tgtcgcgccg tatatcaccg cggtggagaa gcaggcgcgc acggtggcgg
      961 tggatgtgga tttcgcggac ccggccgagg cgcacgggat gctggtgggc tacagcacgg
     1021 acgtggagat cgtgctcgcg acacgtgcgg aggtgctgcg cgtcccgacc ggggccttgc
     1081 gggagggcgg caaggtgctg gtggtcgagg gcgacacgct ggtcgaacgc acgctgcgga
     1141 cgggtgtggc gaactgggag ttcaccgagg tggtgtcggg actggctgcg ggggaaagga
     1201 ttgtgacgtc gctcgagcgg gagggggtga tggcgggggc gaaggtggtg ctgcggccgg
     1261 aagaggcgcg gtaggcgctg gatcgacgtg ggcgccccgg ctcccggatc gcgacttgcg
     1321 tcgctcccac atccctcccc gagcgccgcg gtgtcgccgc ttgcacgcca gtcggcgcac
     1381 gcattcccgg gcgtcgcagc gtcgccgctt ccggtccaat cggcgccagc ctcgccaggc
     1441 gccgcgtctc gcctgtggga gcgacgcaag tcgcgattgc cgccgccgag cgggcaaggt
     1501 cttctttcat tgtccgctcg cccgacaccc catgcagggc gctgaaaggc gcctcatgcc
     1561 acctcacgga agccacaccg gcctgaattg aagctgcaac agaacaccat ggcccagatc
     1621 gaactcgagc acatcgaccg catcttcaag ctcggcgaca gcgaggtgca cgccctgcgc
     1681 gacatcacgc tgcgcatcga gcccggcgag tacgtcgcgg tcatgggacc gtcgggttcc
     1741 ggcaaatcga cgctgctcaa cctgctcggt ctgctcgacc gccccgacgc cggccattac
     1801 cggctcgaag gccgcgacgt caccacactc tcggccgacg agcaggccgc ggtgcgcagc
     1861 gcacgcatcg gcttcgtgtt ccagagcttc cacctcgtcc cccgcctcag cgcagccgag
     1921 aacatcggcc tgccgctggt gctcgccggc atccccgtcg ccgagcgcgc ggctcgcatc
     1981 gcgcaggcct tgcaggactt cggcctcgag gggcgcgccc ggcaccgccc tgacgaactc
     2041 tccggcggcc agcgccagcg cgtcgcgatc gcgcgtgcca ccatcatgcg gccggcgatg
     2101 ctgctcgccg acgagcccac cggcaacctc gaccgcgcca ccggccacga ggtcaccgcg
     2161 ctgctcgagg cgctcaacgc cgccggcacc acgctgatcg tcgtcaccca cgacccttcg
     2221 atgggcgagc gcgcacaccg ccgcatcctg atggaggacg gcgcgatccg tcaggacctg
     2281 cgcaccgccg caccgccccc atgagccccg ccgacaccct gcgcttcgcc acccgcgccg
     2341 ccaccgcgtg gccgctgcgc accgcgttga tgatgctggc gatgtcgatc ggcgtcgccg
     2401 cggtggtggt actcaccgcg ctcggcgacg gcgcgcggcg ctacgtggtg aacgagttct
     2461 ccgcgctcgg tgccagcctg ctgatcgtgc tgcccggacg caccgagacc ggtggcgtca
     2521 acgcgggcag cttcgtcagc agcacgccgc gcgagctcac caacgccgac gccgccgcgc
     2581 tgttgcgctt gccgctggtg cagcgcgtgg cgccgctcgc ggtgggcaac tccgagatcg
     2641 ccgtcggcgg ccggctgcgc gaggtcacgg tcgtcggcac cagcgccgac ttcctcgagc
     2701 tgcgcggtct gaagatcggc cacggcggct tcctgccgcg cgaggacttc aaccgcgcct
     2761 cggcggtggc ggtgatcggc gacgcactgc gcagcgaact cttccccgga cagagcgcgg
     2821 tgggccgcat gatccgtgtc ggcgacaccc ggctgcgcgt gatcggcgtg ctcacgccat
     2881 ccgggcgcgg gctgggcatg accaccgacg agctggtgct ggtgccggtg gcgaccgcgc
     2941 aggcgatgtt cgacaccagc gggctgttcc gcatcttcgt cgaggcgcgc gggcgcgagg
     3001 ccttgcccgc aacgcagcgc cagatcgagg agcgcctgcg cgcgcgccgc gacgacgagc
     3061 tcgacttcac cgtgatcacc caggacgccg ttctcggcac cttcgaccgc atcctcgggg
     3121 cgctcaccct gggcgtggcc ggcatcgccg cgatcagcct ggcagtcgcg ggcatcctgg
     3181 tgatgaacgt gatgctggtc gcggtcaccc agcgcaccgc cgaaatcggc ctgctcaagg
     3241 cgctcggcgc gcgcgccggc accatccgcg ccgccttcct cgccgaggcc gcgctgctct
     3301 ccgtcgccgg cgcgctcgcc ggcttcgcgc tcggccacgc cggcgcctgg gg
//
LOCUS       DAOQNN010000362         3342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_19658_length_3342_cov_11.367204, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000362 DAOQNN010000000
VERSION     DAOQNN010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19658_length_3342_cov_11.367204"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKV97_14570"
     CDS             complement(<1..238)
                     /locus_tag="PKV97_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNB07153.1"
                     /translation="MDPISASGESRAVALARRVLRIEADAVAALGARIGEEFERAVEI
                     ILARHGRVIVTGVGKSGHIGRKLAATLASTGTPAY"
     gene            392..2359
                     /locus_tag="PKV97_14575"
     CDS             392..2359
                     /locus_tag="PKV97_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HNB07154.1"
                     /translation="MLNTLEFVVLLLAAAVVMVALFRSMNLPPVLAYLLVGALGGPHA
                     LDLMQESAGARHLAEFGVVFLMFSIGLEFSLGRLMAMKRIVFGLGLAQVLTTIALATL
                     LGRLGGLGWITSFALGGTLAMSSTAILSKLLADRMELDSRHGREVIGVLLFQDIAVVP
                     LLILLPALSRPAEEMMFTLGLAAFKVVFLLSLVLVFGQRLMRWWFTVMARRRSGELFM
                     LNVLLITLGLAWLSERVGLSLALGAFLAGMLISETEYRYQVEEDIKPFRDVLLGLFFV
                     TVGMLLDVAEIVRNLPAVLGLLGALLVLKFGLVFAASRVFGSPAGTAMRSGLWLCAGG
                     EFGFVLLSQILDLGLMPYGLAQAAVAALVLSMLVAPLIVQVSDRLVMRFVASEWLLRS
                     MELTRVAAQSLNTERHIIVCGYGRSGQYLARFLEQENVAYVALDLDPERVREAGAAGD
                     TVVYGDAARRETLVAAGIQRASALVVSFDALEAALRVIHHTHALRPGLPVVVRAGDEA
                     DLEKLAQAGAAEVVPEAFEASIMLASHALALTGVPLSRVVRRIREIRSQRYALMRGFF
                     QGSSDAGDAGAGAEARLRSVIVPEGAAGVGRSIGELGLEEFGVAVSAVRRRGIRALSP
                     GAETRIQSGDVIVLLGVPGGLEAAEERLLGG"
     gene            complement(2388..3029)
                     /locus_tag="PKV97_14580"
     CDS             complement(2388..3029)
                     /locus_tag="PKV97_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNB07155.1"
                     /translation="MQKKPQTGFTLVEIAIVLLVVGLLLGGVMKGQALIDSARVKNLA
                     QDLRSVPAMVHAYQDRFRALPGDDPAAVRHLCSGGSGCTVAGNGNGVIDGTWDAGADT
                     ESVRLWQHLRLAGIATGSTDTADAGFLPRNALGGRLGVQRGGSVLGLPGSLVVCSDNV
                     PGKLVTQIDIALDDGDPAAGSLRAGTPGDTGLVAISAANRLLEANAHTVCSNL"
     gene            complement(3095..>3342)
                     /locus_tag="PKV97_14585"
     CDS             complement(3095..>3342)
                     /locus_tag="PKV97_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNB07156.1"
                     /translation="TPGARLQIRDHDGRRITTTDSQAVAIVWSTGANLHADGDNTSHA
                     SATPSYQAGEPGADFDDLLAWLGRPLLIARLAQAGRL"
BASE COUNT          394 a         1123 c         1258 g          567 t
ORIGIN      
        1 agtaggccgg cgtgccggtg ctggcgagcg tggcggcgag cttgcggccg atgtggcccg
       61 acttgccaac gccggtgacg atcacccggc catggcgcgc gaggatgatc tcgaccgcgc
      121 gctcgaattc ctcgccgatg cgcgccccga gcgcggccac ggcgtcggcc tcgatgcgca
      181 gcacgcggcg ggcgagggcg acggctcggc tttcgcccga agcggaaatt gggtccatgg
      241 attcgatgct ttaaactggc gcggaaggga gaccggtggc tcgacgggat gacggcgcgc
      301 cgttgccggc aagctcccga tcgagcggat tgtataccaa acacccgcgc cgccgcccgg
      361 cagcgctggc gccacacgcg gccgggcttc catgctgaat accctcgaat tcgtggtcct
      421 gctgctggcc gccgccgtgg tgatggtggc cctcttccgc agcatgaacc tgccgccggt
      481 gctcgcctac ctgctcgtcg gcgcgctcgg cgggccgcat gcgctcgacc tgatgcagga
      541 gtccgccggc gcgcgccacc tcgccgagtt cggcgtggtg ttcctgatgt tctcgatcgg
      601 gctggagttc tcgctcgggc gcctgatggc gatgaagcgc atcgtgttcg ggctcggact
      661 cgcccaggtg ctgacgacga tcgcgctcgc caccctgctc ggtcgcctcg gcgggctggg
      721 ctggatcacc agcttcgcgc tcggcggcac gctggcgatg tcgtccaccg cgatcctctc
      781 caagctgctc gccgaccgca tggagctcga cagccgtcat ggccgcgagg tgatcggcgt
      841 gctgctgttc caggacatcg cggtggtgcc gctgctgatc ctgctgccgg cgctgtcgcg
      901 cccggccgag gagatgatgt tcaccctcgg cctggcggcc ttcaaggtgg tcttcctgct
      961 ctcgctggtg ttggtcttcg gccagcgcct gatgcgctgg tggttcaccg tgatggcgcg
     1021 gcggcgctcg ggcgagctct tcatgctcaa cgtgctgctg atcacgctcg ggctggcctg
     1081 gctgtccgag cgggtcgggc tgtcgctcgc gctcggcgcc ttccttgccg gcatgctgat
     1141 ctcggagacc gagtaccgct accaggtgga agaggacatc aagcccttcc gcgacgtgct
     1201 gctcgggctc ttcttcgtca ccgtcggcat gttgctcgac gtcgccgaga tcgtgcgcaa
     1261 cctgccggcg gtgctcggcc tgctcggcgc gctgctggtg ctcaagttcg ggctggtgtt
     1321 cgcggcctcg cgcgtgttcg gttcgccggc gggcacggcg atgcgctccg ggctgtggtt
     1381 gtgcgcgggc ggcgagttcg gtttcgtcct gctgtcgcag atcctcgacc tcgggctgat
     1441 gccctacggc ctcgcccagg ccgcggtggc cgcactggtg ctgtcgatgc tggtcgcgcc
     1501 gctgatcgtg caggtgagcg accgcctggt catgcgcttc gtcgcctcgg aatggttgct
     1561 gcgctcgatg gagctcaccc gggtggcggc gcagtcgctc aacaccgagc gccacatcat
     1621 cgtgtgcggc tacggacgca gcggccagta cctggcgcgc ttcctcgagc aggagaacgt
     1681 cgcctacgtc gcgctcgacc tcgacccgga gcgcgtgcgc gaggcgggcg cggccgggga
     1741 caccgtggtg tacggcgacg ccgcgcggcg cgagaccctg gtcgccgccg gcatccagcg
     1801 cgcgagcgcg ctggtggtgt ccttcgacgc gctggaggcg gcgctgcggg tgatccatca
     1861 cacccacgcg ctgcgtcccg gcctgccggt ggtggtgcgc gccggcgacg aggccgacct
     1921 cgagaagctt gcccaggcgg gggcggccga ggtggtgccc gaggccttcg aggccagcat
     1981 catgctcgcc tcgcacgcgc tcgcgctcac tggggtgccg ctcagtcgcg tggtgcggcg
     2041 catccgcgag atccgcagcc agcgctatgc gctgatgcgc ggcttcttcc agggcagcag
     2101 cgatgcgggc gatgcgggcg ccggcgcgga ggcgcgcctg cgctcggtga tcgtcccgga
     2161 gggcgcggcg ggagtcgggc gcagcatcgg cgaactcggg ctggaggagt tcggcgtggc
     2221 ggtgtcggca gtgcgtcggc gcggcatccg cgcgctctcg ccgggtgccg agacacgcat
     2281 ccagagcggt gacgtgatcg tgctgctcgg cgtccccggc ggcctggaag ccgccgagga
     2341 gcgcctgctg ggtggctgag ccgcccccgc tccgcacgcc tcacgcctca cagattcgaa
     2401 cacaccgtgt gcgcgttcgc ttcgagcagc cggttggccg cggagatcgc caccagcccg
     2461 gtgtcgccgg gtgtgccggc acgcagcgag ccggccgcgg ggtcgccgtc gtcgagcgcg
     2521 atgtcgatct gtgtgaccag cttgccgggc acgttgtccg agcacaccac caggctgccc
     2581 ggcaggccga gcacgctgcc accgcgctgc acgccgagcc ggccaccgag tgcgttgcgc
     2641 ggcaggaagc cggcgtcggc ggtgtcggtg ctgccggtgg cgatgccggc caggcgcagg
     2701 tgctgccaga ggcggacgga ctcggtgtcg gcgcccgcat cccaggtgcc gtcgatcacg
     2761 ccgttgccgt tgccggccac ggtgcagccg ctgccgccgg agcagagatg gcggacggcg
     2821 gcggggtcgt cgccggggag ggcgcggaag cggtcctgat aggcatggac catcgccggc
     2881 acgctgcgca ggtcctgggc gaggttcttg acacgggcac tgtcgatcag cgcctgcccc
     2941 ttcatgaccc cgccgagcag caggccgacg acgagcagca cgatcgcgat ctcgacgagg
     3001 gtgaatccgg tttgcggttt cttttgcatg gtttgtctcc cgggtgatgt ggcagtggtg
     3061 atggaaaggg ctttgtgcgg atgcgcggcc gggatcacag ccgcccggcc tgggcgagac
     3121 gggcgatcag cagcgggcgc ccgagccagg cgagcagatc gtcgaaatcg gcgcccggtt
     3181 cgccggcctg gtagctcggg gtggcgctgg cgtggctggt gttgtcgccg tcggcgtgca
     3241 ggttggcgcc ggtcgaccac acgatcgcga ccgcctgcga gtcggtcgtg gtgatgcgcc
     3301 ggccgtcgtg atcgcggatc tgcaggcgtg cgccgggtgt gg
//
LOCUS       DAOQNN010000363         3333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_19760_length_3333_cov_10.431361, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000363 DAOQNN010000000
VERSION     DAOQNN010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19760_length_3333_cov_10.431361"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PKV97_14590"
     CDS             <1..863
                     /locus_tag="PKV97_14590"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNB07157.1"
                     /translation="CYHGHADSLLVKAGSGLLTFGNPSSGGVPADFAKHTIVLDYNDL
                     QQVEDVFKARGDEIAAIIVEPVAGNMNLIKPQPGFLEGLRRICTEYGAVLIFDEVMTG
                     FRVGPQGVQGLYGITPDLTTLGKVIGGGMPVGAFGGRRDIMEKIAPLGSVYQAGTLSG
                     SPVAVAAGMVSLKLTREAGFYERLTASTQRLVAGLAAAAKDAGVTFSADSVGGMFGVY
                     FADKVPASFAQVMATDKERFNRFFHAMLEAGHYFAPSAFEAGFVSVAHGEAEIDTTVA
                     VARAVFAQLG"
     gene            complement(930..2372)
                     /gene="mgtE"
                     /locus_tag="PKV97_14595"
     CDS             complement(930..2372)
                     /gene="mgtE"
                     /locus_tag="PKV97_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNB07158.1"
                     /translation="MTEPEELHADDVQQHLKEVQDLLARQKVVEGLVHRQDMPRHELV
                     ENLVHKQHEAGLRAKLDSLHPADIAYILEALPREERLYVWDLVKAERDGEILLEVSDA
                     VRESLIETMDPEELKAAAGSLDADELADLAPDLPPEVIEDVFQSLDSEGREQLRAAMS
                     YPEDSVGALMDFDMVTVRDDVTLEVVLRYLRRFDELPDHTDKLFVVDREDHLEGILTL
                     ETLLISDPEKTVAEVMRSEPVISFTPEDEADDAAQAFERYDLVSAPVIDSERRVIGRI
                     TVADAVDFIREESEAEILSQAGLREEEDIFASVWSSVKNRWAWLAINLVTAFVASRVI
                     GLFEESIEKLVALAALMPIVAGIGGNSGNQTVTMIVRAIAMGQVEPSAMTRLLRKELG
                     VALINGVVWGGLLGVLAWWLYGNVDLGLVMTGAMTLNLLLAACAGVLVPMLRQRLGAD
                     PALGGSVMITALTDSGGFFIFLGLATLFLL"
     gene            complement(2507..3214)
                     /gene="mtgA"
                     /locus_tag="PKV97_14600"
     CDS             complement(2507..3214)
                     /gene="mtgA"
                     /locus_tag="PKV97_14600"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNB07159.1"
                     /translation="MPIPAPRRILGWVARGLGVALLLALFWQAVLLTQVVWWSRFDPG
                     STSFMRIRLAELREADPRAELRQQWVPYERISIHLKRAVVAAEDDRFLDHDGFDWEGI
                     ERALERNAEEGRVTAGGSTISQQLAKNLFLSSSRSWLRKGQEAAITLMIEATWDKRRI
                     LEVYLNVAEWGNGVFGAEAAARHYYGRPAASLGPAEAARLAVMLPNPRRYEKTFGPRL
                     AAHAARVQRRMANSQVP"
     gene            complement(3222..>3333)
                     /gene="aroE"
                     /locus_tag="PKV97_14605"
     CDS             complement(3222..>3333)
                     /gene="aroE"
                     /locus_tag="PKV97_14605"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371937.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNB07160.1"
                     /translation="LGMLVEQAAESFALWRGVRPDSAPVLAELRQLIEAG"
BASE COUNT          507 a         1171 c         1151 g          504 t
ORIGIN      
        1 gctgctacca cggccacgcc gacagcctgc tggtgaaggc cggctccggc ctgctgacct
       61 tcggcaaccc gtcctcgggc ggcgtgccgg cggacttcgc caagcacacc atcgtgctcg
      121 actacaacga cctgcagcag gtcgaggacg tgttcaaggc gcgcggcgac gagatcgccg
      181 cgatcatcgt cgagccggtg gccggcaaca tgaacctgat caagccgcag cccggcttcc
      241 tcgaaggcct gcgccgcatc tgcaccgagt acggcgcggt gctgatcttc gacgaggtga
      301 tgacggggtt ccgcgtcggc ccgcagggcg tgcagggcct ctacggcatc acccccgacc
      361 tgaccacgct cggcaaggtg atcggcggcg gcatgccggt gggcgccttc ggcggtcgtc
      421 gcgacatcat ggaaaagatc gccccgctgg gcagcgttta ccaggccggc accctgtcgg
      481 gcagcccggt ggcagtggcg gcgggcatgg tgtcgctgaa gctgacgcgt gaggccggct
      541 tctacgagcg gctgacggcg agcacgcagc gcctggttgc gggtctggcc gcggcggcca
      601 aggatgccgg cgtcacgttc agcgcggact cggtcggcgg catgttcggc gtctattttg
      661 ccgacaaggt gccggcctcc ttcgcccagg tgatggctac ggacaaggag cgcttcaacc
      721 gcttcttcca cgccatgctg gaagccggcc actacttcgc gccctcggcc ttcgaggccg
      781 gcttcgtgtc ggtggcgcat ggcgaggccg agatcgacac cacggtggcc gtggcgcgcg
      841 cggtcttcgc ccaactgggc tgagccttcc cgcaggcctg cggagcgggc tcgcccgctc
      901 ctggcgccat attcctcaca tctcgcccgt cagagcagga aaagcgtcgc cagcccgagg
      961 aagatgaaga agccgcccga gtcggtgagt gcggtgatca tcaccgagcc gccgagcgcg
     1021 gggtcggcgc cgaggcgctg gcgcagcatc gggaccagca cgcccgcgca ggctgccagc
     1081 aggaggttca gcgtcatcgc accggtcatc accaggccga gatcgacgtt gccgtagagc
     1141 caccacgcca gcacgccgag cagtccgccc cacaccacgc cgttgatcag cgccacgccg
     1201 agctccttgc gcagcaggcg ggtcatcgcc gagggctcga cctggcccat cgcgatcgca
     1261 cgcacgatca tcgtcacggt ctggttgccc gagttgccgc cgatgccggc gacgatcggc
     1321 atcagcgccg cgagcgcgac cagcttctcg atcgactcct cgaacaggcc gatcacgcgc
     1381 gaggccacga aggcggtgac gaggttgatc gccagccagg cccagcggtt cttcaccgag
     1441 ctccacaccg aggcgaagat gtcctcctcc tcgcgcaggc cggcctggga caggatctcg
     1501 gcctcggact cctcgcggat gaagtccacc gcgtcggcca cggtgatgcg gccgatcacc
     1561 cgccgctcgg aatcgatcac cggcgccgac accaggtcgt agcgctcgaa ggcctgggcg
     1621 gcgtcgtcgg cctcgtcctc gggggtgaag ctgatcaccg gctcggagcg catcacctcg
     1681 gccacggtct tttccgggtc gctgatcagc agcgtctcca gggtcaggat gccctcgagg
     1741 tggtcctcgc ggtcgaccac gaagagcttg tcggtgtggt cggggagttc gtcgaagcgg
     1801 cgcaggtagc gcagcacgac ctccagggtg acgtcgtcgc gtaccgtcac catgtcgaag
     1861 tccatcagcg cgccgacgga atcctccggg taggacatcg ccgcgcgcag ctgctcgcgg
     1921 ccctcgctgt cgagcgactg gaagacgtcc tcgatgacct cgggcggcag gtcgggggcg
     1981 aggtcggcga gctcgtcggc atccagcgaa cctgccgccg ccttgagctc ctcggggtcc
     2041 atcgtctcga tgagcgactc gcgtaccgcg tcggagacct cgagcaggat ctcgccgtca
     2101 cgctctgcct tgaccaggtc ccagacatag agacgctcct cgcgcggcag cgcctcgagg
     2161 atgtaggcga tgtcggccgg gtgcagactg tcgagcttgg cgcgcagccc ggcctcgtgc
     2221 tgcttgtgca cgaggttctc caccagctcg tggcgcggca tgtcctggcg atggacgaga
     2281 ccctccacca ccttctggcg ggcgagcaga tcctgcacct ccttgaggtg ctgctggacg
     2341 tcgtcggcgt ggagttcttc gggctcggtc atcgcgcgca aggccggcgg aggggcaagt
     2401 cgcgaattct acggactccc ccccgcgcac gcgaccccga tcatgtgaca gaaggcgaaa
     2461 aatcggcgga gggccattcc cgcccctcgc gccttgcgcg tcgcgctcaa ggcacctgcg
     2521 aattcgccat ccgccgttgc acccgcgccg cgtgcgcggc caggcggggg ccgaaggtct
     2581 tctcgtagcg ccgcgggttg ggcagcatca ccgccaggcg cgcggcctcg gccgggccca
     2641 ggctggcggc cgggcggccg tagtagtggc gcgcggcggc ctcggcaccg aacacgccgt
     2701 tgccccattc ggccacgttc aggtacacct cgaggatgcg gcgcttgtcc caggtcgcct
     2761 cgatcatcag ggtgatcgcc gcctcctggc ccttgcgcag ccaggagcgc gaggacgaca
     2821 ggaagaggtt cttggcgagc tgctggctga tcgtcgagcc gccggcggtg acgcggccct
     2881 cctcggcgtt gcgctccagg gcgcgctcga tgccctccca gtcgaagccg tcgtggtcga
     2941 ggaagcgatc gtcctcggca gcgactaccg cgcgcttgag gtggatcgag atgcgctcgt
     3001 agggcaccca ttgctggcgc agttcggccc gcgggtcggc ttcgcgcaac tcggcgaggc
     3061 ggatgcgcat gaagctcgtg ctgccgggat cgaagcggct ccaccacacc acctgggtca
     3121 gcagcaccgc ctgccagaac agcgccaaca gcagcgcgac acccagcccg cgggcgaccc
     3181 agccgaggat gcggcgcggc gcggggatcg gcacgctggc ctcagccggc ttcgatgagc
     3241 tggcgcagct cggccagcac cggcgcggag tcggggcgca cgccgcgcca cagcgcgaag
     3301 ctctcggcgg cctgctcgac cagcatgccc agc
//
LOCUS       DAOQNN010000364         3323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_19845_length_3323_cov_5.406365, whole genome shotgun sequence.
ACCESSION   DAOQNN010000364 DAOQNN010000000
VERSION     DAOQNN010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19845_length_3323_cov_5.406365"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..2670
                     /locus_tag="PKV97_14610"
     CDS             223..2670
                     /locus_tag="PKV97_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNB07161.1"
                     /translation="MRIIALAFLAGTVLVQRSAALDGVVAAAALGLVAALGLRLALVL
                     AWGRGSAARAGRWIPIALAALGALAAGAGWAAWRAEVRLSDELAGTLDGVELQLRGRV
                     AGLPRASGSGWRFDFEVEPDTPGVPGLVQLAWRPERTVVEAKLDAEADSASPLPLAMP
                     RPGERWALTVRLWRPHGFANPSGFDYEAWLLERGVRATGSVRGTGRLLDELPATWMQH
                     VHRVRATVRERILAALPDGAHAGLLVALAVGDQQGIDAGQWEIFRRTGVAHLVSISGL
                     HVALVAMFCGGLAGAAWRRQPALVLRVPVQQVHALGGLAGATAYALLAGMGVPVLRAW
                     LMLLVGALALIGGRRIPPSRVLAIALLVVLVVDPWAVLSAGFWLSFGAVAVILAVLGG
                     RVAPSAGWRAALRVQLAISFALVPLLLAQFQAVPLLSPLANLVAIPLVSFVVTPLVLL
                     AIPWPIPLLLVPADFAAGWMMDFLAPLAALERAMIERAAPPPWLFGGALAAVAILLLP
                     RASPGRLAAPALLAALLSWQPARPQEGGFRAWVLDVGQGLAVHVQTRAHDLLYDSGPP
                     FGSESDAGERVVLPWLHAAGVRRLDRVVLSHPDSDHVGGAASVLAGLPVGTVLAGQAV
                     HARERAAWAARLAHGREPADCARVPAWERDGVRFEILHPAPVRAPSEEPAGAGASPHD
                     NDASCVLRVVGAAGALLLTGDIGTAAEAKLIARQPPASLSAAVVVSAHHGSRSSSSAA
                     FVDATLPEHAVHSAGSRNSFGHPHPEVWARWAEAGARNWRTDAQGAIRIDFAAAPEEG
                     VQVNAEREMQPRYWHGR"
     gene            2753..>3323
                     /locus_tag="PKV97_14615"
     CDS             2753..>3323
                     /locus_tag="PKV97_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07162.1"
                     /translation="MSLLDSLKKFFSPSAPTTPAERAAVEPERVRAAQPLLQPRARHV
                     LCSGPHGLHRVAYTEWGEPTNRRVLVCVHGLTRNGRDFDDLARALAGEYRVVCPDVVG
                     RGRSDWLTDKADYGFPVYARAVSPGTTLGRYRTVASQVPGIACHGEIFNPDHLGGPAV
                     PDVLGLTMAERVADPAALLARVRKAPGLNG"
BASE COUNT          335 a         1200 c         1255 g          533 t
ORIGIN      
        1 tctcgctgac gctgcggtgg tcggggagga agcgtttgaa gagcttgcgc atggttcagg
       61 tccgcgtggg cgagtctagc aggagcccgg cccatcgcgt cgtggcatgc gcggccatcc
      121 tcgacgcggc ggcccgggtc gcgatcgtcc gtcgagggcg ggatggcggc cggcgatcac
      181 ggcgcccgcc cttccgggaa tgcctgaggc atactcgatg ccatgcgcat catcgctctc
      241 gccttcctcg ccggcaccgt cctcgtccag cggtcggctg cgctcgatgg cgtcgtggcc
      301 gcggcggcgc tcggcctcgt cgctgcgctc ggcttgcggc tggcgttggt gttggcgtgg
      361 ggccgcgggt cggcggctcg ggctgggcgc tggatcccca tcgccctggc cgcactcggt
      421 gcgctcgccg ccggcgcggg ctgggcggcg tggcgggccg aggtccggct gagcgacgag
      481 ctcgccggca ccctcgacgg tgtcgagttg cagctgcggg gcagggtggc cgggctgcca
      541 cgcgcgagcg ggtccggctg gcgcttcgac ttcgaggtcg agcccgacac gcccggcgtg
      601 ccgggactcg tgcagctggc ctggcgtccc gaacgcaccg ttgtcgaggc caagctcgac
      661 gccgaggccg attccgcgag ccccctgccg ctggcgatgc cccgtccggg cgagcgctgg
      721 gcgctgaccg tgcgtctgtg gcggccccac ggtttcgcca atccctccgg cttcgactac
      781 gaggcctggt tgctcgagcg cggggttcgc gccacgggca gcgtgcgggg gacggggcgg
      841 ctcctagacg agcttcccgc cacctggatg cagcacgtcc atcgcgtgcg cgcgacggtg
      901 cgcgagcgca tcctcgccgc cctgcccgac ggggcgcacg ccggcctgtt ggtggcgctc
      961 gcggtcggtg atcaacaagg catcgatgcc gggcagtggg agatcttccg ccgcaccggc
     1021 gtcgcccacc tggtgtccat ctcgggcctg cacgtggcgc tggtggcgat gttctgcggc
     1081 ggtctcgccg gcgcggcgtg gcgccggcag ccggcgctgg tgctgcgtgt tccggtgcag
     1141 caggtgcacg cgctcggcgg gctggccggg gccaccgcct atgccttgct ggccggcatg
     1201 ggcgttccgg tgctgcgcgc ctggctgatg ctgctggtgg gcgcgctggc gctgatcggc
     1261 gggcggcgca taccgccctc gcgcgtactc gcgatcgcat tgctcgtggt gctggtggtc
     1321 gatccctggg cggtgctgtc ggccggcttc tggctgtcct ttggtgcggt ggcggtgatc
     1381 ctcgccgtgc tcggcggccg tgtcgcaccg tcggccggct ggcgcgccgc gctgcgcgtg
     1441 cagctcgcga tcagcttcgc gctggtgccg ctgctgcttg cgcagttcca ggcggttccg
     1501 ttgctgtcgc cgctcgccaa cctggtggcg attccgctgg tgagcttcgt cgtcacgccg
     1561 ctggtgctgc tcgcgatccc ctggccgatc cccttgctgc tcgtgccggc cgacttcgcc
     1621 gcggggtgga tgatggattt ccttgcgccg cttgcagcgc tcgagagggc gatgatcgag
     1681 cgcgcggcgc cgccgccgtg gcttttcggc ggtgcgctcg ccgccgtggc gatcctgctg
     1741 ctgccgcgcg caagcccggg gcggttggcg gcaccggcat tgctcgcggc gctgctgtcc
     1801 tggcagccgg cgcgcccgca ggagggcggt ttccgcgcct gggtgctgga cgtcggccag
     1861 ggtctcgcgg tgcatgtgca gacgcgtgcg catgacctcc tctacgacag cgggccaccc
     1921 ttcggcagcg agtccgatgc cggcgagcgg gtggtcctgc cctggctgca cgcggccggc
     1981 gtgcgccggc tggatcgggt ggtgctgtcg catccggatt ccgatcatgt gggcggcgcc
     2041 gcctcggtgc tcgccggcct gccggtgggc acggtgctgg ccggacaggc cgtccatgcg
     2101 cgcgaacgcg cagcctgggc ggcccgcctg gcgcacgggc gcgagcctgc ggactgcgca
     2161 cgcgtgcccg catgggagcg cgatggcgtg cgcttcgaga ttctgcatcc ggcgcccgtg
     2221 cgcgcgccga gcgaggagcc ggcaggcgcg ggcgcaagtc cgcacgacaa cgacgcctcc
     2281 tgcgtgctgc gcgtcgttgg cgccgccggc gcgctgctgc tgaccggcga catcggcacc
     2341 gccgccgagg ccaagctgat cgcccgccag ccaccggcaa gcctttccgc cgcggtcgtg
     2401 gtcagcgccc accatggcag ccgctcgtcg tcctcggccg ccttcgtcga cgcgaccctc
     2461 ccggaacacg cggtccactc cgccggcagc cgcaacagct tcggccatcc ccaccccgag
     2521 gtgtgggcac gctgggcgga ggcaggggca cgcaattggc gcaccgatgc gcagggtgcg
     2581 atccggatcg acttcgcggc ggcgccggag gagggcgtcc aggtgaacgc cgagcgcgag
     2641 atgcagccgc gctactggca cggtcgctga gggcggcggg cttgtgtccc ggatccgccg
     2701 cttcgggcta gacttgcagc gacccgaaac ccgttccgag tgcccgcccc tcatgtccct
     2761 gctcgattcg ctcaagaagt tcttcagccc gtccgcaccg acgaccccgg ccgagcgtgc
     2821 cgcggtggag ccggagcgtg tgcgcgccgc gcaaccgctg ctccagccgc gcgcccggca
     2881 cgtgctgtgc agcgggccgc acggcctgca ccgcgtggcc tacaccgaat ggggcgagcc
     2941 gacgaatcgg cgcgtgctgg tctgtgtcca cggtctcacc cgcaacggcc gcgacttcga
     3001 cgatctcgcg cgcgcgctcg ccggcgagta ccgcgtcgtc tgcccggacg tggtcgggcg
     3061 cgggcgcagc gactggctga cggacaaggc ggactacggc ttcccggtct acgcgcgcgc
     3121 cgtcagcccg ggcaccacgc tcggccgcta caggacggtg gcgtcgcagg tgccgggcat
     3181 cgcctgccac ggcgagatct tcaatcccga tcatctcggc ggcccggccg tgccggacgt
     3241 gctgggcctg acgatggccg agcgcgtcgc cgatccggcc gcccttctcg cccgcgtccg
     3301 caaggccccc gggctcaacg gct
//
LOCUS       DAOQNN010000365         3319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_19889_length_3319_cov_11.609069, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000365 DAOQNN010000000
VERSION     DAOQNN010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19889_length_3319_cov_11.609069"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..503)
                     /locus_tag="PKV97_14620"
     CDS             complement(3..503)
                     /locus_tag="PKV97_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNB07163.1"
                     /translation="MPSLAWSADLDNNLAPMDETHREFVECYNAAANAAPDDFLRALD
                     ALIEHAKAHFDLENGWMEAVDFPGCHRAEHDRVLAVMADIRKRVERGDHFLGRRLMEE
                     LPAWFVNHVNGMDAALAFHLATIGFDVDSCTLRPLAEGETRAAGCGCATLAAEDAAAG
                     EAARAA"
     gene            complement(513..983)
                     /locus_tag="PKV97_14625"
     CDS             complement(513..983)
                     /locus_tag="PKV97_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HNB07164.1"
                     /translation="MSTVSTKPYLLRAIWEWCVDQGFTPYLATLVDEDTRVPPGYARD
                     GQIVLNLSPDATGQLQMANDFISFQARFGGAAHSLVIPVANVVAIYARENGQGMAFEP
                     ELEAVEDASVAVQDPSADVVQDMSDTTAELRRERSAEPQPPARPPRGSHLKIVK"
     gene            complement(1048..1644)
                     /locus_tag="PKV97_14630"
     CDS             complement(1048..1644)
                     /locus_tag="PKV97_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNB07165.1"
                     /translation="MMNLYSGTTDPFSHRCRIVLFEKGMDFEVIDVDLYNKPEDIAVI
                     NPYNRVPVLVDRDLVLYEANIINEYIDERFPHPQLMPPDPIMRARARQLLHTFEQELF
                     THLEALEAAQKGVDKVRTHVRDHLTQLAPVFTKQKYMLGEEFSMLDVAIAPLLWRLEH
                     YGIELPKAAAPLMKYAERIFSRQGFIDALTPSEKVMRR"
     gene            complement(1752..2507)
                     /locus_tag="PKV97_14635"
     CDS             complement(1752..2507)
                     /locus_tag="PKV97_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HNB07166.1"
                     /translation="MKTRVINFIKRFAAVALFAPAVAMAAGASVHLDKAPVSSEPAAL
                     QHGAKLFVNYCMNCHSASFVRYNQLQNIGLSEAEIRDNLMFTGERVGDLMKIAMRPAE
                     SKGWFGVAPPDLTLVARQRASADGSGADWLYTYLRQFYRDPSRPTGWNNAIFANVAMP
                     HVFWQLQGEQVAKITQNADGTTTTALELVKPGKLSVQEYDKAVADLVSFLVWMGEPVA
                     EKRKSIGALVLIFLAGMFVLSYALKKNYWKDIH"
     gene            complement(2504..>3319)
                     /locus_tag="PKV97_14640"
     CDS             complement(2504..>3319)
                     /locus_tag="PKV97_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNB07167.1"
                     /translation="WGQMSFWGAQVIVNLFSAIPVIGPDLSLVIRGDYVVSDATLNRF
                     FSFHVIAVPLVLIGLVLAHIVALHEVGSNNPDGVEIKKKKDATGKPLDGIPFHPYYTV
                     KDIVGVVVFLFFFSAVVFFMPEGGGYFLEFNNFIPADPLKTPPHIAPVWYFTPFYSIL
                     RAVTYPLFGLDAKFWGVVAMGASVVIIAFLPWLDRSPVKSIRYKGPIFKMVLVVFVIA
                     FFILGYLGVLPPTPGRTLVSQICSVIYFAFFLLMPWYSKLDKCKPEPERVNFK"
BASE COUNT          597 a         1034 c         1125 g          563 t
ORIGIN      
        1 catcaggccg cgcgcgcggc ctcgccggct gccgcatcct cggcagcgag cgtggcgcag
       61 ccgcagccgg cggcgcgcgt ctcgccttcg gcgagtgggc gcagcgtgca cgaatcgacg
      121 tcgaagccga tcgtcgccag atgaaaggcg agcgcggcat ccatgccgtt cacgtgattc
      181 acgaaccagg ccggcagctc ttccatcagt cggcggccaa ggaagtggtc gccgcgctcg
      241 acgcgcttgc ggatgtcggc catcaccgcg agcacgcggt catgctcggc gcgatggcag
      301 ccgggaaagt cgacggcctc catccagccg ttctcgagat cgaagtgggc cttcgcatgc
      361 tcgatgagcg cgtcgagcgc acgcaggaaa tcatcgggcg cggcgttggc cgcggcgttg
      421 tagcactcga cgaactcgcg atgggtctcg tccatcgggg cgaggttgtt gtcgagatcg
      481 gcgctccagg caaggctagg catcggtgtt cctcacttga cgatcttgag atggctgccg
      541 cgcggcgggc gcgcgggggg ctgcggctcc gcggagcgct cgcgacgcag ttcggcggtg
      601 gtgtcgctca tgtcctggac gacgtcggcg gacggatcct gcaccgccac ggacgcatcc
      661 tccaccgcct cgagctcggg ctcgaaggcc atgccctgcc cgttctcgcg tgcgtagatc
      721 gcgaccacgt tggccaccgg gatcaccagc gaatgcgcgg caccgccgaa acgggcctgg
      781 aagctgatga agtcgttggc catctgcagc tggccggtgg cgtcgggcga aaggttcagc
      841 acgatctggc cgtcgcgcgc gtaaccgggc ggcacccggg tgtcctcgtc caccagcgtg
      901 gcgagatagg gcgtgaaacc ctgatccacg caccattccc agatggcgcg caacaggtat
      961 ggcttggtcg agacggtgct catcggctct ccggtggccg tgccacgccc ctgcgggcgc
     1021 cgcacggcct gactgcggtc gtgcggctca gcgccgcatg accttttcgg acggggtcag
     1081 cgcgtcgatg aatccctgac ggctgaagat gcgttcggcg tacttcatca gcggcgcggc
     1141 ggccttgggc agttcgatgc cgtagtgctc gaggcgccac agcagcgggg cgatcgcgac
     1201 gtcgagcatg gagaactcct cgccgagcat gtatttctgc ttggtaaaca ccggcgcgag
     1261 ctgggtgaga tggtctcgca cgtgggtgcg caccttgtcc acgcccttct gcgcggcctc
     1321 cagtgcctcc aggtgggtga acagctcctg ctcgaaagtg tgcaacagct ggcgggcacg
     1381 cgcacgcatg atcgggtcgg gtggcatcag ctgcggatgc gggaagcgct cgtcgatgta
     1441 ctcgttgatg atgttggcct cgtagagcac gaggtcgcga tcgaccagca ccggcacgcg
     1501 gttgtacggg ttgatgacgg cgatgtcttc gggcttgttg tagaggtcga cgtcgatgac
     1561 ctcgaagtcc atgccttttt cgaacagcac gatccggcaa cgatgactga agggatcggt
     1621 cgtgccggaa tacaggttca tcatggtgtt acgactccgg aattcaaaag caaaagcgca
     1681 ccacgcgggt ccgcgtggtg cgtcagggtc aggaccgcac tagccggccc ggagggccgc
     1741 ggcgacagga atcaatgaat gtccttccag tagttcttct tcagcgcgta gctgagcacg
     1801 aacatgccgg cgaggaagat cagcacgagg gcgccgatcg acttgcgctt ctcggccacc
     1861 ggctcgccca tccagaccag gaaggagacg aggtcggcga ccgccttgtc gtactcctgg
     1921 accgacagct tgcccggctt gacgagttcg agggccgtcg tcgtcgtgcc gtccgcattc
     1981 tgcgtgatct tggcgacctg ctcgccctgc agctgccaga acacgtgcgg catggccacg
     2041 ttggcgaaga tggcattgtt ccagccggtc gggcggctcg ggtcgcggta gaactggcgc
     2101 agataggtat acagccagtc ggccccgctg ccgtccgccg aggcgcgctg ccgcgccacc
     2161 agcgtcaggt cgggcggcgc gacgccgaac caccccttgg actccgccgg ccgcatcgcg
     2221 atcttcatca ggtcgccgac gcgctcgccg gtgaacatta ggttgtcgcg gatctccgcc
     2281 tcggagaggc cgatgttctg cagctggttg tagcgcacga agctggcgct atggcagttc
     2341 atgcagtaat tgacgaacag cttggcgccg tgctgcagcg cggccggctc gctgctcacc
     2401 ggcgccttgt ccagatgcac gctggcaccg gcggccatcg ccacggccgg cgcgaacagc
     2461 gccacggccg cgaaccgctt gatgaagttg atcactcgcg ttttcatttg aagttcaccc
     2521 tttccggttc gggtttgcac ttgtcgagct tgctgtacca cggcatcagc aggaagaagg
     2581 cgaagtagat caccgagcag atctgcgaca ccagcgtgcg acccggggtc gggggcagaa
     2641 cacccaggta acccaggatg aagaaggcga tcacgaagac gaccagcacc atcttgaaga
     2701 tcggcccctt gtagcggatc gacttgacgg ggctgcggtc cagccagggc aggaaggcga
     2761 tgatcaccac cgacgcgccc atggccacca cgccccagaa cttcgcatcg agaccgaaca
     2821 gcggataggt cacggcgcgc aggatcgaat agaagggggt gaaataccac accggcgcga
     2881 tgtgcggcgg ggtcttcagc ggatcggccg ggatgaagtt gttgaactcg aggaagtagc
     2941 caccgccctc gggcatgaag aacacgaccg cactgaagaa gaacaggaag acgacgacgc
     3001 cgacgatgtc cttcaccgtg tagtacgggt ggaaggggat gccgtcgagc ggcttgccgg
     3061 tcgcatcctt cttcttcttg atctcgacac catcggggtt gttggagccg acttcgtgca
     3121 gcgcgacgat gtgcgcgagc accaggccga tcagcaccag cggcacggcg atgacgtgga
     3181 aggagaagaa gcggttcagc gtggcgtcgg agacgacgta gtcgccgcgg atcaccagcg
     3241 acaggtccgg accgatcacc gggatcgccg agaacaggtt cacgatcacc tgggcgcccc
     3301 agaacgacat ctggcccca
//
LOCUS       DAOQNN010000366         3311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_19966_length_3311_cov_9.062961, whole genome shotgun sequence.
ACCESSION   DAOQNN010000366 DAOQNN010000000
VERSION     DAOQNN010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19966_length_3311_cov_9.062961"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="PKV97_14645"
     CDS             complement(<1..1265)
                     /locus_tag="PKV97_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNB07168.1"
                     /translation="MRLPALLLACVLMVPAAFAAAPQAGARPDHVADRDCLACHPDQA
                     DAWAESKHRHAMQPATAQTVLGDFGEAGSGEVRFGEGAAAARFFRRGGDFMVAAQGPD
                     GRTQALAASHVFGIRPLQQVLLPQQGGRLQAFTIAWDTTRKAWFSLHADGPVAPGDNL
                     HWSGRYQNWNLMCGECHTTAYRKGYDDEHDRYATTWAEANVGCQACHGAGRAHAESAR
                     KLAAAGERGQAPLHTPNRAQAGAHAQVDQCAACHARRTRLVEDAVAGAPLFDQFIPDN
                     LRPGLYHADGQQLDEVFEYGSYRQSRMYQAGVACTDCHDPHRGRLRAEGNALCTACHN
                     PAPDRGRFPGLQAKDYDAPSHHFHRGGGAGSQCVDCHMPSRNYMVVHPRRDHAIRVPR
                     PDLGARTGAPDACTGCHRPTARSATSRRWA"
     gene            complement(1270..3018)
                     /locus_tag="PKV97_14650"
     CDS             complement(1270..3018)
                     /locus_tag="PKV97_14650"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNB07169.1"
                     /translation="MKAALATLLIPVATLAIGVQAGHAASPVQHDRPNILLIMADDLG
                     YTDLGSYGSEIATPNLDTLADTGVKMTQFYASPFCSPTRAMLMSGTDNHLAGFGDMAE
                     LMLPEQRGKPGYEGYLNERVVPMAQVLRDAGYRTLMTGKWHLGVPEQYSPAARGFDQS
                     YALVHGGSSHWSDGAGIVAADPAKPPKAIYRENGKETTLPKDFFSSDFFTSRLIEYID
                     AGKGSGKPFFAYLAFTAPHWPLHAHDADIARYEQRYKDGYDKLRRERLERMKKLGLVA
                     ADTPVFEGHPLWPKWDSLSAAEKESEARRMAVYAAMVDNMDQNIGRMLDYLKKTGQLD
                     NTFIFFLSDNGADGNSVYDVARTREWIHKDMDNSIAHIGKSGSYAEYGPGWAQVGSTP
                     FRMFKSFMYEGGIAVPAIAWGPGVKGGKLESAMAHVTDIAPTLFELAGAKHPGTEYQG
                     RPVLPLRGASMLPLLQGRGQAVHGADKAIGWELGGRKALRKGDWKIVSANQPWGTGDW
                     ELFNVAQDRSESRNLAAANPQKLGEMLVAWRDYVRETGTLEIPNLANRPGYSNGAKYY
                     EDLKYEATLVPRTAKP"
     gene            complement(3015..>3311)
                     /locus_tag="PKV97_14655"
     CDS             complement(3015..>3311)
                     /locus_tag="PKV97_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07170.1"
                     /translation="AQEVGDRPDEGGEGLLVHGVWVVGRGSPAIVGRTIGRPTLDCLA
                     RDTGPDIRRAAELPSSLGPTFHPAEHFQIAQLFSRIELNSWNRPEAGLIPGDRQ"
BASE COUNT          419 a         1110 c         1233 g          549 t
ORIGIN      
        1 gcccatcgcc gcgacgtagc gcttcgcgct gtcggccggt ggcagccggt gcaggcgtcg
       61 ggcgcgccgg tgcgcgcgcc caggtcgggg cgcggcaccc ggatggcgtg gtcgcggcgc
      121 ggatgcacga ccatgtagtt gcggctcggc atgtggcagt cgacgcactg gctgccggcg
      181 ccgccgccgc ggtggaagtg atgcgagggc gcgtcgtagt ccttcgcctg caggccgggg
      241 aagcgaccgc ggtcgggcgc gggattgtgg caggcggtgc acagcgcgtt gccttcggcg
      301 cgcaggcgac cgcggtgagg gtcgtggcag tcggtgcagg ccacgcccgc ctggtacatg
      361 cggctctggc ggtaggagcc gtactcgaag acctcgtcga gctgctggcc gtcggcgtgg
      421 tagaggccgg ggcgcaggtt gtcggggatg aactggtcga acagcggcgc gccggccact
      481 gcgtcctcga ccagccgggt gcggcgcgcg tggcaggcgg cgcactggtc gacctgggcg
      541 tgtgcgccgg cctgcgcccg gttcggggta tgcagcggcg cctggccgcg ctcgcccgcg
      601 gcggcgagct tgcgtgcgct ctcggcgtgc gcgcggccgg cgccgtggca ggcctggcag
      661 cccacgttcg cctcggccca ggtggtggcg tagcggtcgt gctcgtcgtc gtagcccttg
      721 cggtaggcgg tggtgtggca ctcgccgcac atcaggttcc agttctggta gcgcccgctc
      781 cagtgcaggt tgtcgcccgg ggcgaccggg ccgtcggcgt gaagcgagaa ccaggccttg
      841 cgcgtggtat cccaggcgat cgtgaaggcc tgcaggcgtc cgccctgctg cggcagcagc
      901 acctgctgca gcggccggat gccgaagacg tggctggccg cgagcgcctg cgtgcggccg
      961 tcgggccctt gcgcggcgac catgaagtcg ccgccgcggc ggaagaagcg cgccgccgcc
     1021 gcaccctcgc cgaagcggac ttcgccggag ccggcctctc cgaagtcgcc gagcacggtc
     1081 tgcgccgtcg ccggctgcat cgcatggcgg tgcttcgatt cggcccaggc gtccgcctgg
     1141 tcggggtggc aggcgaggca gtcacggtcg gcgacgtggt cgggccgcgc ccccgcttgc
     1201 ggggcggccg cgaacgcggc cggcaccatc aggacgcagg cgaggagcag ggcgggcagg
     1261 cgcatgcgct cagggcttgg ccgtgcgcgg gacgagggtg gcctcgtact tgaggtcctc
     1321 gtagtacttc gcgccgttgc tgtagccggg gcggttggcg aggttgggga tctccagcgt
     1381 gccggtctcg cgcacgtagt cgcgccaggc caccagcatc tcgcccagct tctgcgggtt
     1441 ggcggcggcg aggttgcggc tctcgctgcg gtcctgcgcg acgttgaaga gctcccagtc
     1501 gcccgtgccc cagggctggt tcgccgacac gatcttccag tcgcccttgc gcagcgcctt
     1561 gcgcccgccc agctcccagc cgatcgcctt gtcggcgcca tgcacggcct gcccgcgccc
     1621 ctgcagcagc ggcagcatcg aggcgccgcg cagcggcagc acgggcctgc cctggtactc
     1681 ggtgccgggg tgcttcgcgc cggcgagctc gaacagcgtg ggcgcgatgt cggtcacgtg
     1741 ggccatcgcc gactcgagct tgccgccctt gacgcccggg ccccaggcga tcgccggcac
     1801 ggcgatgccg ccctcgtaca tgaaggactt gaacatgcgg aacggcgtcg aacccacctg
     1861 cgcccagccc ggcccgtact cggcgtagga gccggacttg ccgatgtgcg cgatgctgtt
     1921 gtccatgtcc ttgtggatcc actcgcgggt gcgcgccacg tcgtacaccg agttgccgtc
     1981 ggcgccgttg tcggacagga agaagatgaa ggtgttgtcg agctgcccgg tcttcttcag
     2041 gtagtcgagc atgcggccga tgttctggtc catgttgtcg accatcgcgg cgtacaccgc
     2101 catgcgcctg gcctcggatt ccttctccgc cgcgctcagg ctgtcccact tcggccacag
     2161 cggatggcct tcgaacaccg gcgtgtcggc ggccaccagg ccgagcttct tcatgcgctc
     2221 gaggcgctcg cggcgcagct tgtcgtagcc gtccttgtag cgctgctcgt acctggcgat
     2281 gtcggcgtcg tgcgcgtgca gcggccagtg cggcgcggtg aaggcgaggt aggcgaagaa
     2341 gggcttgccc gaacccttgc cggcgtcgat gtactcgatc agccgcgagg tgaagaagtc
     2401 ggacgagaag aagtccttcg gcagcgtcgt ctccttgccg ttctcgcggt agatggcctt
     2461 cggcggcttg gccggatcgg cggcgacgat gcccgcgccg tcgctccagt ggctggagcc
     2521 gccatgcacc agcgcatacg actggtcgaa gccgcgcgcg gccgggctgt actgctcggg
     2581 cacgccgagg tgccacttgc cggtcatcag cgtgcgatag ccggcgtcgc gcagcacctg
     2641 cgccatcggc accacgcgct cgttgaggta gccctcgtag cccggcttgc cgcgctgctc
     2701 gggcagcatc agctcggcca tgtcgccgaa gccggcgagg tggttgtcgg tgccggacat
     2761 cagcatcgcg cgcgtcggcg agcagaacgg cgaggcgtag aactgggtca tcttgacccc
     2821 ggtgtcggcc agggtgtcga ggttgggagt ggcgatttcg ctgccgtagc tgccgaggtc
     2881 ggtgtagccg aggtcgtcgg ccatgatcag caggatgttg gggcggtcgt gctggaccgg
     2941 cgaggcggcg tggccggcct ggacgccgat ggcgagcgtt gccaccggga tcagcagggt
     3001 cgcgagcgct gctttcattg tctgtcccct ggaataagcc cggcttctgg ccggttccat
     3061 gaatttaatt caattctcga gaaaagctgc gcgatctgaa aatgttccgc agggtggaac
     3121 gtcgggccaa gactggacgg cagttctgcc gcccgccgga tgtccggccc ggtgtcccgt
     3181 gcgaggcagt cgagcgtggg ccggccaatc gtcctgccca caatcgcagg cgatccccgc
     3241 ccgacgaccc agaccccatg aaccagcaag ccctctccgc cctcatctgg tcggtcgccg
     3301 acctcttgcg c
//
LOCUS       DAOQNN010000367         3298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_20093_length_3298_cov_12.270737, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000367 DAOQNN010000000
VERSION     DAOQNN010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20093_length_3298_cov_12.270737"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1465
                     /locus_tag="PKV97_14660"
     CDS             272..1465
                     /locus_tag="PKV97_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HNB07171.1"
                     /translation="MDYQIQRLKVLGAGIFSLMLALGVARFAYTPLLPLMQAQAGLGL
                     AEGGWLAAINYTGYLSGALLAASISDLVLKDRLYRIGMVVAVLTTLMMGLTTDFTVWA
                     VSRYLAGLSSAAAMLLGTGLILNWLIRHNHRHELGIHFAGIGLGIAGCSVAVALMSLW
                     LDWRAQWFVFTAIACVLLVPALRWLPAPDTSGLTRSGAPMHDDPPSPLFLRIFMASYF
                     CAGVGFVVSATFIVAIVNRLPGLEGQGSWSFLAIGLAAMPACIVWDFIARRTGALNAL
                     ILAAVLQIVGILLPVVVGGSLGAIAGALLFGGTFVGMVSLVLTMAGRYYPTRPAKMMG
                     KMTISYGVAQILGPAVTGWLGETFGSYAGGLWFAAAMMGVGTVLLVLLKIVDRRDAQA
                     AAGVA"
     gene            complement(1441..2292)
                     /gene="nudC"
                     /locus_tag="PKV97_14665"
     CDS             complement(1441..2292)
                     /gene="nudC"
                     /locus_tag="PKV97_14665"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HNB07172.1"
                     /translation="MSLPSSLPPSGDPRPARTLVFRGHQLLVDLAATPTAGPDSWRAG
                     LVALRTLDFPVTTGEAWTVVEVERTIEAPDGHAFQHLKSLLDTLDGETLALAARALEL
                     LEFDRAHRFCGACGAPTTLRTDPPARRCSAPDCGREHFPRVSPVVIVAVERGAEILLG
                     RSPHFPPGLYSTLAGFVDAGESAEQAVHREIFEETGLRIRAPRYFSSQAWPFPHALML
                     GYQAEYAGGDIVCAPGEIEDARFFHVDALPPLFPIRYAMANQLLRDFCLRHGRPWPPA
                     QATPAAA"
     gene            2594..>3298
                     /locus_tag="PKV97_14670"
     CDS             2594..>3298
                     /locus_tag="PKV97_14670"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNB07173.1"
                     /translation="MSAGKSKIIYTLTDEAPLLATCAFLPIIRTFTGPAGVEVEKADI
                     SVSARVLAEFPEYLSEDQRVPDTLAELGRLTLEPDTNIIKLPNISASVAQLKACVKEL
                     QEKGYKIPDYPEDPKTDEEKALRVRFGKCLGSAVNPVLREGNSDRRAPAAVKNYAKKH
                     PHSMGEWKQWSQTHVSHMHHGDFYHGEKSMTLDRARDVKMELITKSGKTIVLKPKVSL
                     LEGEIIDSMFMSKKALC"
BASE COUNT          537 a         1064 c         1173 g          524 t
ORIGIN      
        1 agggggccag cagcgtaaac aggagcagca ggaagactcg gatcagcggg cgcatcggga
       61 agattctgtg gacgtggcag gagatgggcc tgcgggaggc gtggggcgga tcggcgtgcg
      121 cggcaacacc gggtccaggg gccgaagttt agccggtcgc caggagccgg gggcgcgtgg
      181 ccggatgaaa gcttcgtgtc gaaacatttg cttgaatgga tacgactccc cgtctatcgt
      241 gtcgcgtccg gaggagatgg aaaacgacaa gatggactac cagatccagc gtctgaaggt
      301 gctcggtgcg ggcatcttca gcctgatgct cgcgctcggc gtggcgcgct tcgcctatac
      361 cccgctgctg ccgctgatgc aggcccaggc cgggctcggg ttggccgagg gcggctggct
      421 cgcggcgatc aattacaccg ggtacctgag cggtgcgctg ctcgccgcct cgatcagcga
      481 cctcgtgctc aaggaccgcc tctaccgcat cggcatggtg gtcgcggtgc tgaccacgct
      541 gatgatgggg ctcaccaccg atttcacggt gtgggcggtg tcgcgctacc tggccggact
      601 ttcgagtgcg gcggcgatgt tgctcggcac gggcctgatc ctgaactggc tgatccgtca
      661 caaccatcgc cacgagctcg gcatccactt cgccggcatc ggtctgggca tcgccggctg
      721 ctcggtggcc gtggcgctga tgagcctgtg gctggactgg cgtgcgcagt ggttcgtgtt
      781 caccgcgatt gcctgcgtgc tgctggtgcc ggcgctgcgc tggctgccgg cacccgacac
      841 cagcgggctg acgcgcagcg gcgcgccgat gcacgacgac ccgcccagcc cgctcttcct
      901 gcgcatcttc atggcgtcct acttctgtgc cggcgtcggc ttcgtggtca gcgcgacctt
      961 catcgtcgcc atcgtcaatc gcctgcccgg gttggagggc caggggagct ggagctttct
     1021 cgccatcggc ctggcggcga tgccggcctg catcgtgtgg gatttcatcg cccgccgtac
     1081 cggcgcgctg aacgcgctga tcctcgccgc ggtgctgcag atcgtcggca tcctgctgcc
     1141 ggtggtggtc ggtggcagcc tgggggcgat cgccggcgcc ctgctcttcg gcggcacctt
     1201 cgtcggcatg gtcagcctgg tgctgaccat ggccgggcgt tactacccga cgcggccggc
     1261 caagatgatg ggcaagatga ccatctccta tggcgtcgcg cagatcctcg gcccggcggt
     1321 gaccggctgg ctgggcgaga ccttcggcag ctacgcaggc gggctgtggt tcgccgcggc
     1381 gatgatgggc gtgggcaccg tgttgctggt gctgctgaag atcgtggacc ggcgcgacgc
     1441 tcaggccgcg gcaggcgtcg cctgagcggg cggccacggt cgcccgtggc gcaggcagaa
     1501 gtcgcgcagc agctggttgg ccatcgcgta gcggatcggg aacagcggcg gcagcgcgtc
     1561 gacatggaag aagcgcgcat cctcgatctc gcccggagcg cagacgatgt ccccgccggc
     1621 gtactcggcc tggtagccga gcatcagcgc atgcgggaag ggccacgcct ggctggagaa
     1681 atagcgtggc gcgcggatgc gcaggccggt ctcctcgaag atctcgcgat gcaccgcctg
     1741 ctcggccgat tcgcccgcgt ccacgaagcc ggcgagcgtg ctgtagaggc cgggcgggaa
     1801 gtgcggcgag cgcccgagca ggatctcggc accgcgctcg accgccacga tcaccaccgg
     1861 cgagacgcgc ggaaagtgct cgcgaccgca gtccggcgcg ctgcagcgcc gcgccggcgg
     1921 atccgtccgc agtgtggtgg gcgcgccgca ggcgccgcag aagcggtgcg cgcggtcgaa
     1981 ctcgagcagc tcgagggcgc gcgcggcgag ggcgagcgtc tccccgtcaa gcgtgtcgag
     2041 cagcgacttg agatgctgga acgcatgtcc gtcgggtgcc tcgatcgtgc gctcgacctc
     2101 gaccaccgtc catgcctcgc ccgtggtcac ggggaagtcg agggtgcgca gcgcgacgag
     2161 accggcccgc caggaatccg ggccggccgt gggcgtcgcc gcgaggtcca cgagcaactg
     2221 gtggccgcgg aacacgagcg tgcgtgccgg ccgcggatcg ccggatgggg gcagtgaaga
     2281 aggaaggctc atgatcgtgg gaccggggtc gggcggagcg tcggcgcaag gaggaaggcg
     2341 agggcggcag catcgggcag atcgcccgtg ctgtcgagtc tgtggcgcgg gtcgtgaccg
     2401 cggggctcgt ttccaggccc ggcaaggctt gccgcggttt atttcgcgat gtgaaagaag
     2461 gtttcgatgc ccgacaggtc gataagtggt atttttccgg gcgactgcga tcgtgcggcg
     2521 gccttgccgc gccggcgaca gtgggtcgcc atcgggtagt cgggacaaga tctaaaacat
     2581 ataggggaaa tcaatgtctg caggcaaatc caagatcatc tacacgctca cggacgaagc
     2641 gccgctgctg gcgacgtgcg ccttcctccc gatcatccgc accttcaccg gtcctgcagg
     2701 cgtcgaggtc gagaaggccg acatctccgt gtcggcgcgc gtgctcgccg agttccccga
     2761 atacctgagc gaagaccagc gcgtccccga cacgctcgcc gaactgggcc gcctgaccct
     2821 cgagcccgac accaacatca tcaagctgcc caacatcagc gcctcggtcg cccagctcaa
     2881 ggcttgcgtc aaggaactgc aggagaaggg ctacaagatc cccgactacc ccgaggaccc
     2941 caagaccgac gaggaaaagg cgctccgcgt ccgcttcggc aagtgcctcg gctcggccgt
     3001 caacccggtg ctgcgcgagg gcaactccga ccgtcgcgcg cccgccgcag tcaagaacta
     3061 cgccaagaag cacccgcact ccatgggcga gtggaagcag tggtcgcaga cccacgtctc
     3121 ccacatgcac cacggcgact tctaccacgg cgagaagtcg atgacgctcg accgcgcgcg
     3181 cgacgtcaag atggagctga tcaccaagtc gggcaagacc atcgtgctca agcccaaggt
     3241 ctcgctgctc gaaggcgaga tcatcgactc gatgttcatg agcaagaagg cgctgtgc
//
LOCUS       DAOQNN010000368         3298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_20094_length_3298_cov_7.626580, whole genome shotgun sequence.
ACCESSION   DAOQNN010000368 DAOQNN010000000
VERSION     DAOQNN010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20094_length_3298_cov_7.626580"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKV97_14675"
     CDS             complement(<1..309)
                     /locus_tag="PKV97_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase"
                     /protein_id="HNB07174.1"
                     /translation="MRQAERALFDLRRGLSILVRDHARAFVVCAVEGLDDAAAGEMQM
                     LAGSPARLVLSDHRLAAIGRAGAAQAVSVGLSPLPDAAGLHALAWQRGASLPADAELR
                     D"
     gene            509..1273
                     /locus_tag="PKV97_14680"
     CDS             509..1273
                     /locus_tag="PKV97_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNB07175.1"
                     /translation="MAIEIASRGAWRCARALRVDTAELLDLFLPWVARPGRLAIAQLG
                     QSLDGRIATASGASHYINCLEARTHLHRLRALVDAVVVGVGTVNADDPQLTVRHVAGR
                     NPVRVVLDPRGRARADARLFQDGAAPTVHLVGEDLAPGTAAVLPGRVARAGLPGAGSG
                     FEPGAVLDWLARAGCRRVLVEGGGHTVSRFVQAGAIERLHLLVAPLLIGSGRAGLVLP
                     PIDTLDEALRPVCRPFRCGSDSLFDLDFDGAGRAAQ"
     gene            1472..2407
                     /locus_tag="PKV97_14685"
     CDS             1472..2407
                     /locus_tag="PKV97_14685"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB07176.1"
                     /translation="MSIDLPSNPHQGPLPTRASATARPVVHDLPRAREAGARPASVQR
                     LDLCRLVADVVPDEAPPNPAEQYEALVLVRGHAFLRSSEAAIELEPGQILLGTGASPE
                     LLYSADSELWSVLVPESVLREACSECGWLHAGEGVRFNRSPCTRDELDHLVTLLRLVA
                     SESASGALRAQLFMHYAHAVANKLLFTLQHNQPTSQTCGQARLFERLERHIEARIKQD
                     ISVEQLAQFAGLSQRSLYQVFKDQARMSPRTYIRRKKLEHVYATLMDPAVRVASVTAV
                     ALDYGFTHLGRFAELYKSSFGILPSESLKARPPGK"
     gene            complement(2382..3296)
                     /locus_tag="PKV97_14690"
     CDS             complement(2382..3296)
                     /locus_tag="PKV97_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNB07177.1"
                     /translation="MLAGVLWWIEPRALIAAFGAPAPAWLALALAIGVLQTALSAWRW
                     QLTAGRLGAPLGFAHALREYYLASFLNQILPGGVMGDAARAWRHASLPGIARDAAWQA
                     VVIERGAGQLALLLVVVASVLAAPALQVVPGRLGDAVDLRGLPWMGVLAVLALVATAL
                     AGRTRTALHSLASATRQALLGRAVLLRQLLASLLIVASYVAVWLCCMRMSGLATPPAQ
                     AAALVPWVLLAMAIPLSVAGWGIREGAAALVWQAAGLDAAEGVAASVSYGVVVLLSTL
                     PGALALRRHEPGARSTAEATPTCRADGP"
BASE COUNT          477 a         1161 c         1206 g          454 t
ORIGIN      
        1 gtcgcgaagt tcggcgtccg cgggcaggct ggcgccgcgc tgccaggcga gcgcatgcag
       61 accggcggcg tcgggaagcg gcgacaggcc gacggaaacc gcctgcgcgg ccccggcgcg
      121 gccgatcgcg gcgaggcggt ggtcgctcag caccaggcgt gcgggcgagc cggccagcat
      181 ctgcatttcg cccgccgcgg catcgtccag gccttcgacc gcgcacacga cgaaggcgcg
      241 cgcgtggtcg cgcacgagga tcgaaagtcc gcgtcgcagg tcgaaaaggg cacgctcggc
      301 ttggcgcatt tgtttttctc cgtttggggt gtcggggctc gaccctgcac caggcgcttt
      361 ccaggccggc agacgcgagt gaagacgatt tttcagggaa ggatggatgg cggacgaact
      421 tgtaccagat tggggctggg cctgcatcct gcaagcccgc gatgctgatt gggccggcgc
      481 cgggacgata agtttcacgg tcgagggcgt ggcgatcgag atcgcttcgc gcggcgcctg
      541 gcgctgcgcg cgggccttgc gcgtggacac cgcggaactg ctcgatctct tcctgccctg
      601 ggtggccagg cccggccgtc tcgcgatcgc ccagctgggg cagagcctgg atggccgcat
      661 cgccaccgcg agcggggcct cgcactatat caactgcctc gaggcacgta cccacctgca
      721 ccggctgcgg gcgctggtgg atgcggtggt ggtgggggtc ggcacggtga atgccgacga
      781 tcctcagctc acggtgcgcc acgtcgccgg gcgcaacccc gtgcgcgtgg tgctcgaccc
      841 gcgcggtcgc gcgcgggcgg acgcgaggct gttccaagac ggcgccgcgc ccaccgtgca
      901 cctggttggc gaggatctcg cgccgggcac cgccgccgtg ctgccggggc gggtcgcgcg
      961 cgcgggcctg ccaggtgccg ggtcgggttt cgagccgggc gcggtgctcg attggctggc
     1021 acgggcgggc tgcaggcggg tgctggtgga ggggggtggg cacaccgtgt cgcgtttcgt
     1081 gcaggcgggg gcgatcgagc ggctccatct gctggtcgca ccgctgctga tcggctcggg
     1141 gcgcgccggg ctggttctgc cgccgatcga cacgctcgac gaggccctgc gtccggtgtg
     1201 caggccgttc cgctgcggga gcgacagctt gttcgacctc gacttcgatg gggcggggcg
     1261 ggcggcgcag tagagatgaa atctcgatat aaaataaaaa catcgacaaa cgcgtttctc
     1321 gtcgacgaat gactgatatg atcccgcagg tcttcgtaat cgtttccgtg cgatcctgaa
     1381 tcgttcggct gcgctgcggg acaaccggcg ctccacggcg agtgggcgac ggaccccgct
     1441 gcagccggct cgccgattca ctggagtgag gatgtcgatc gacctgccgt ccaatccgca
     1501 tcaaggcccg ctcccgacgc gggcctccgc aaccgcccga cctgtggtgc acgatctgcc
     1561 gcgggcgcgg gaagccggcg cgcgtccggc gagtgtgcag cgcctggacc tgtgccggct
     1621 ggtcgccgat gtcgtgcccg acgaggcccc gcccaacccc gcggagcaat acgaggccct
     1681 cgtgctcgtg cgcgggcacg ccttcctgcg cagcagcgag gcggcgatcg agctggagcc
     1741 cggacagatc ctgctcggca ccggtgcgtc gcccgagctg ctctattcgg ccgatagcga
     1801 gctgtggagt gtgctcgtcc ccgagtccgt gctgcgcgaa gcgtgttcgg agtgcggctg
     1861 gctccacgcg ggagagggcg tgcgcttcaa ccgcagcccc tgcacgcgcg acgagctcga
     1921 ccacctggtc acgctgctgc gcctggttgc gagcgagagc gccagcgggg cgctacgcgc
     1981 gcagctgttc atgcactacg cccatgccgt ggccaacaag ctgctgttca ccctgcagca
     2041 taaccagccg acctcgcaga cctgcggcca ggcgcgcctg ttcgagcgcc tcgagcgcca
     2101 catcgaggcg cgcatcaagc aggacatcag cgtcgagcaa ctcgcccagt tcgccgggct
     2161 gagccagcgc tcgctctatc aggtgttcaa ggaccaggcg cggatgtcgc cgcgaaccta
     2221 catccgccgc aagaagctcg agcatgtgta cgccaccctg atggacccgg ccgtgcgcgt
     2281 ggcgagcgtc accgcggtgg cgctcgacta cggctttacc cacctcgggc gcttcgccga
     2341 gctctacaag tcgagcttcg gcatcctgcc ctcggagagc ctcaaggccc gtccgcccgg
     2401 caagtaggcg tcgcctcggc ggtggagcgc gcgccgggct cgtggcgccg cagcgccagc
     2461 gcgcccggca gcgtcgacag cagcacgacc acgccgtagc tcaccgaggc cgccacgcct
     2521 tcggcggcgt ccagcccggc cgcctgccac accagcgcgg ccgcgccttc acggatgccc
     2581 cagcccgcca ccgagagcgg gatcgccatc gccagcagca cccagggcac cagcgccgcc
     2641 gcctgggctg gcggcgtggc gagcccgctc atgcgcatgc agcacagcca caccgcgacg
     2701 tagctggcca cgatcagcag cgaggcgagc agctggcgca gcagcaccgc gcgcccgagc
     2761 agcgcctgcc gggtggcgct cgccaggctg tgcagggccg tgcgggtgcg gcctgcgagt
     2821 gccgtcgcga cgagggcgag cacggcgagc acgcccatcc acggcagtcc ccggaggtcg
     2881 acggcgtcgc ccaggcgtcc cggcacaacc tgcagcgcgg gggcggcgag cacgctcgcg
     2941 accaccacca gcagcagcgc gagctggccc gcgccgcgct cgatcaccac tgcctgccag
     3001 gctgcgtcgc gggctatgcc cggcagactg gcgtggcgcc aggcgcgcgc ggcgtcgccc
     3061 atcaccccgc ccggcaggat ctggttgaga aagctcgcga ggtagtactc gcgcagcgca
     3121 tgggcgaagc cgagcggggc gccgaggcgg ccggcggtga gctgccagcg ccacgccgac
     3181 aaggccgtct gcagcacgcc gatcgcgagc gcgagcgcga gccaggcggg cgcgggcgcg
     3241 ccgaaggcgg cgatgagggc gcgcggttcg atccaccaca gcacgccggc gagcagcc
//
LOCUS       DAOQNN010000369         3288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_20196_length_3288_cov_26.125580, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000369 DAOQNN010000000
VERSION     DAOQNN010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20196_length_3288_cov_26.125580"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1823)
                     /locus_tag="PKV97_14695"
     CDS             complement(<1..1823)
                     /locus_tag="PKV97_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07178.1"
                     /translation="MAKSAATDEQLLAAISAAEQASVSMSEGQLATDRADAIDRFNGK
                     TYGNERPGRSAVVSRDVADVVEGVLANVIKPFVAGDQVVQFNPRGPEDENAAQQETDY
                     VNFVALERNNGFVVLASSFKDALLLRNGYIKCGWTKREDVMVERYEGQSDDQLAILLS
                     DPEIEVVQHSEYPDPSFVPPPMPAPQAMQAGFTPDMPPQQMLHDVVVRRKSSTEYVET
                     VPVPPDEIVISERTRTPSVQDTPFIQHRTHKTLSELRQLGYDVPDDIADDDKGETQEG
                     YARDRFSFTGDDEDEHVNDPSRREVLFKESWIRFDYDGDGIAELRRVCSVGQEVLSNE
                     ETDLIPIAAFTGTIMSHQHLGVSLYDQVKDVAELKTAVLRQFMDNKYLANNARTIVDV
                     DRVNLDDLLESRPGGIVRVNGDPASAVVPMVTPDTGASALQALEYIDSIRENRTGYTR
                     YAQGMGSDSLINKTATGLMQASNQSQMRLEMVSRTIAETGIRDLFRIIHALTLKHANK
                     AEKVRLRNKWVEVDPRTWARRTDLQISVGLGSGNSDQMFGKLMTMVPLMQQAQAMGLA
                     GPQEAYNLGAELWKAAGFKNPDKFLHPPAVDPKTGQPQQPQP"
     gene            complement(1824..3236)
                     /locus_tag="PKV97_14700"
     CDS             complement(1824..3236)
                     /locus_tag="PKV97_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07179.1"
                     /translation="MQASLETLPQPSPDSERAEAERTILRWRAEGPALFAQQALGASP
                     TEQQWQASRELVKTRRVSIRSGHGTGKSAFMAWTILWALVCYHPVKVPVTAPTGHQLS
                     DVLWAELAMWWRKMPPDLMAEFEWAAERFYRKDVPQEAFAAARTSRPEKPEALQGFHA
                     ENVVFVIDEASGIPDEVFQVAEGALSTDGAFVVMAANPTRMDGYFYDSHHKQRGRWAA
                     LHWNAEDSPLVSRDYIEDMAAKYGRESAIYRIRVRGEFAGNPDGVIPLDLIEGAISRE
                     VNAYGPERWGLDVARFGEDRTALAKRHGNAMLVPVKSWRGKDLMQTAGLVKIEYDNAQ
                     RKPEAICVDVIGLGAGVADRMRELGLPVVAVNVAESPSASERYNRLRDELWFKAREWF
                     AGRDVKLVQDDELTAELTMPNYKVLSTGKIQVESKDEIKKRAGGNRSPDLADAFCLTF
                     AMGGSKRKWAPLKYNDTGIV"
BASE COUNT          517 a         1140 c          999 g          632 t
ORIGIN      
        1 ggctgcggct gctgcggctg gcccgtcttg ggatcgacgg ccggcgggtg caggaacttg
       61 tccgggttct tgaagcccgc cgccttccag agctcggccc cgaggttgta agcctcctgc
      121 ggcccagcaa ggcccatcgc ctgcgcctgc tgcatcagcg gcaccatcgt catgagcttg
      181 ccgaacatct ggtccgagtt acccgacccc agcccgaccg agatttgcag gtcggtgcgc
      241 cgcgcccacg tccggggatc gacctcgacc cacttgttcc gcagccgcac cttctcggct
      301 ttgttcgcgt gcttgagcgt cagcgcgtgg atgatgcgga acaggtcgcg gatgcccgtc
      361 tcggcaatcg tccggctgac catctcaagc cgcatttgcg actggttgga cgcctgcatc
      421 agccccgtag cggtcttgtt gatgagcgag tccgacccca tgccctgcgc gtagcgcgtg
      481 tacccggtgc ggttctcgcg gatgctgtcg atgtactcca gcgcctgcag ggccgacgcc
      541 ccggtgtcag gcgtcaccat cggtacaacc gccgacgcgg ggtcgccgtt aacgcgaaca
      601 atgccgcccg gccgcgactc caacaggtcg tcgaggttca cccgatcaac gtctacgata
      661 gtcctcgcat tattggcgag gtacttgttg tccatgaact gccgcaggac cgccgtcttg
      721 agttcggcaa cgtccttcac ctggtcgtac aggctgacgc ctaggtgctg gtgcgacatg
      781 atcgtgccag tgaaggcggc gatcggaatc aggtcggttt cctcgttcga cagcacttcc
      841 tgacccacgc tgcacacgcg tcgcagctcg gcaatcccgt cgccgtcata gtcgaaccga
      901 atccagctct ccttaaacag cacctcacgc cgagacgggt cgtttacgtg ctcgtcctcg
      961 tcgtcgccgg tgaacgagaa gcggtcgcgg gcgtagccct cttgcgtctc gcccttgtcg
     1021 tcgtcggcaa tgtcgtcggg tacgtcgtag cccaactgcc gcagctccga gagcgtcttg
     1081 tgcgtccggt gctggatgaa cggcgtgtcc tgcacgctcg gcgtgcgcgt ccgctcagag
     1141 attacgatct cgtccggcgg gacggggacc gtctcgacgt actcggtgct tgacttccgg
     1201 cgcaccacaa cgtcatgcag catttgctgc ggcggcatgt cgggcgtgaa cccagcctgc
     1261 atcgcctgcg gggccggcat cggcggcggg acaaacgacg ggtcaggata ctccgagtgc
     1321 tgcaccacct cgatctcggg atcggacagc aggatggcca actggtcgtc agactggccc
     1381 tcgtaccgct cgaccatcac atcttcacgc ttcgtccagc cgcacttgat gtagccattc
     1441 cgcagcagca gggcgtcctt gaacgacgac gccagcacca cgaatccgtt attacgctcc
     1501 agagcgacga agttaacgta gtccgtctcc tgctgggccg cgttctcgtc ctccggcccg
     1561 cgggggttga actgcaccac ctgatcgccg gccacgaacg gcttgatgac gttcgccagc
     1621 acgccctcga ccacatccgc cacatcgcgg gagacgaccg cagaccggcc cggccgctcg
     1681 ttgccgtacg tcttgccatt gaaccggtca atggcgtccg cgcggtcggt cgcaagttgc
     1741 ccctccgaca tgctcacgga cgcctgttcg gcggccgaga tcgcggctag caactgctcg
     1801 tcagtggcgg cggatttggc caattagacg attcccgtat cgttgtactt gagcggcgcc
     1861 cacttgcgct tgctgccgcc catagcgaag gtcaggcaga aggcgtcggc aaggtcgggc
     1921 gacctgttgc cgccggcccg cttcttgatc tcgtccttgc tctcgacctg aatctttccg
     1981 gtcgatagca ccttgtagtt aggcatggtc agctcggcgg tcagctcgtc atcctgaaca
     2041 agcttcacgt cccgcccggc gaaccattcg cgcgccttga accagagttc atcgcgcagc
     2101 cggttgtatc gctcgctcgc gctcggggac tcggccacgt tcaccgcaac caccggcagg
     2161 cccagctcgc gcatacggtc ggccacgcca gcgcccagcc caatcacgtc cacacagata
     2221 gcctcgggct tgcgctgcgc gttgtcgtac tcgatcttga ccagcccggc ggtctgcatc
     2281 agatccttgc cgcgccagga cttgaccggc accagcatcg cattgccgtg ccgcttggcg
     2341 agcgccgtgc ggtcctcgcc gaagcgggca acgtccagcc cccagcgctc gggaccgtat
     2401 gcgttcacct cgcggctgat ggccccctcg atcaagtcca gcgggatcac gccgtcgggg
     2461 ttgccggcga actcgccacg cacccggatg cggtagatcg cggactcgcg gccgtacttc
     2521 gccgccatgt cctcgatgta gtcccggctg actagcgggc tatcctcggc gttccaatgc
     2581 agcgccgccc accgaccacg ctgcttgtgg tggctgtcgt agaaatagcc atccatccgc
     2641 gtcgggttgg ccgccatcac gacgaacgcg ccgtcggtgg acagcgcgcc ctcggccacc
     2701 tggaacacct cgtcgggtat ccccgaggct tcgtcgatca cgaatacgac gttctcggcg
     2761 tggaagccct gcagcgcctc gggcttctcc ggcctcgacg tacgggcggc cgcgaaggct
     2821 tcttgcggta cgtccttgcg gtagaaccgc tccgcagccc actcgaactc ggccatcagg
     2881 tcgggcggca tcttgcgcca ccacatggcg agctccgccc acagaacgtc ggacagttgg
     2941 tgccccgtcg gagccgtcac cggcaccttg accgggtgat agcagacgag cgcccacagg
     3001 atcgtccacg ccataaaggc cgacttgccg gtgccgtggc ccgaccggat agacacccgc
     3061 cgggtcttga ccagctcacg gctggcctgc cattgctgct cggtgggact tgcccccagc
     3121 gcctgctggg cgaacagggc cgggccttca gccctccacc gcaggatcgt tctttccgct
     3181 tccgctcttt ccgagtccgg cgatggttgc ggtaaggttt ccagtgacgc ttgcattgat
     3241 ctccgagggg atcagcttgg acgcaagctt gtagaagtcg gtgggatt
//
LOCUS       DAOQNN010000370         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_20222_length_3286_cov_9.222532, whole genome shotgun sequence.
ACCESSION   DAOQNN010000370 DAOQNN010000000
VERSION     DAOQNN010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20222_length_3286_cov_9.222532"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..739)
                     /gene="infC"
                     /locus_tag="PKV97_14705"
     CDS             complement(191..739)
                     /gene="infC"
                     /locus_tag="PKV97_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNB07180.1"
                     /translation="MATFLDRRTPNAERKHRLNREITAPEVRLNGPENEPLGIVSIQE
                     ALRLSAEHDVDLVEIAPTAQPPVCRLMDYGKFKYQEQKKAAEAKAKQHVIDVKEVKFR
                     PGTDEGDYNIKMRNLRRFLEDGDKGKVTLRFRGREITHQDIGMRLLERIRDELADVSL
                     VEHMPKLEGRQMIMVLGPKKKK"
     gene            complement(820..2733)
                     /gene="thrS"
                     /locus_tag="PKV97_14710"
     CDS             complement(820..2733)
                     /gene="thrS"
                     /locus_tag="PKV97_14710"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNB07181.1"
                     /translation="MISIQLPDGSRREFDGPVTVAEVAASIGAGLAKAALAGRVGSGA
                     EARLVDISHRIEADTQLAIVTAKDADGLDIIRHSTAHLLAYAVKSLFPDAQVTIGPTI
                     EHGFYYDFAYKRPFTPEDLAAIEKKMAELAAKDEPVVRSVLPRNEAVAYFKSIGEAYK
                     AEIIEGIPADQDVSLYAEGSFTDLCRGPHVPSTGKLKFFKLMKVAGAYWRGDHRNEML
                     QRVYGTAWATKDDLQKYLTMLEEAEKRDHRKLGRELDLFHLDEHSPGTVFWHPKGWAI
                     WQEVEQYMRRVYRDNGYQEVKGPQILDKGLWEKTGHWDKYRENMFTTESEKRDYALKP
                     MNCPGHILIYKQGIKSYRDLPLRYGEFGACHRNEPTGGLHGIMRVRGFTQDDGHIFCT
                     EDQILPECDAFTALLKKVYADFGFTDILYKVATRPDKRIGSDELWDRAEAALIASLRS
                     SGCEFEISPGEGAFYGPKIEYTLKDALGRHWQCGTIQVDFSLPERLGAEFVAESGERQ
                     HPVMLHRAILGSLERFIGILIEEHAGALPLWLAPTQVAVMNITDAQADYAQDVAKALQ
                     KQGLRVALDLRNEKITYKIREHSLQKVPFLLVVGDKEKAAGAVAVRARGNQDLGVMAL
                     EAFSQKLAAELTR"
     gene            complement(2994..>3286)
                     /locus_tag="PKV97_14715"
     CDS             complement(2994..>3286)
                     /locus_tag="PKV97_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNB07182.1"
                     /translation="CIVDSNSDDIVYSNLFTGVHGNYLKPSIRAAGLDPDHLPESDPT
                     KMNFGGDAKKAWKDIWGCGQGIGAIDKIRPAGALVAQLAAEYAAAKARVCGG"
BASE COUNT          524 a         1069 c         1050 g          643 t
ORIGIN      
        1 cgaaaacgct tcttcgcgct gctcttggtc ttcatcttgg gcatgactgc tccttttgct
       61 agttcccgta cggcgccgcg aaactgatcg cgaacttggg ggccgacggg caccgggcgg
      121 cagcagcgac caaaccgcta ccacctcgtc tttcatgtct gccagaccgg accaccggtc
      181 gggcagccaa tcacttcttc ttcttcgggc cgagcaccat gatcatctgg cgcccttcga
      241 gcttgggcat gtgctcgacc agcgagacat cggccaactc gtcgcgaatg cgctccagca
      301 ggcgcatgcc gatgtcctgg tgcgtgatct cgcggccgcg gaagcgcagc gtgaccttgc
      361 ccttgtcgcc gtcttccagg aagcggcgca ggttgcgcat cttgatgttg tagtcgcctt
      421 cgtccgtgcc cggacggaac ttgacttcct tgacgtcgat gacgtgctgc ttggccttgg
      481 cctcggccgc cttcttctgc tcctggtact tgaacttgcc gtagtccatc aggcggcaga
      541 ccggcggctg cgcagtcggc gcaatctcca ccagatccac atcgtgctcg gccgacaggc
      601 gcagagcctc ctggatgctg acgatgccca ggggctcgtt ctccggtccg ttcaggcgca
      661 cctcgggagc ggtgatctcg cggttcaggc gatgcttgcg ctcggcgttg ggcgtacggc
      721 ggtcaagaaa ggtagcgatg gtccaattcc tccagggccc ggggccccac gatcaatgca
      781 gcgcacccaa gcaaagagca acgaaccacc gacccaccct cagcgggtca actcggcagc
      841 gagcttctgg gagaaggcct ccagggccat cacaccgagg tcctgattgc cccgggcgcg
      901 cacggcgacg gctccagcag ccttttcctt gtcaccgaca acgagcagga acggaacctt
      961 ctgcagcgaa tgctcgcgta ttttatacgt gatcttttcg ttgcgcaaat caagtgcgac
     1021 cctaagtcct tgtttttgca gcgctttggc cacgtcctgg gcgtagtcgg cctgcgcgtc
     1081 ggtgatgttc atgaccgcga cctgcgtcgg cgcgagccac aaaggcagtg cgccggcgtg
     1141 ttcctcgatc aggatgccga tgaaacgctc cagactgccg aggatcgcgc ggtgcagcat
     1201 caccgggtgc tggcgctcgc cggactcggc gacgaactcg gcgcccaggc gctcgggcag
     1261 cgagaaatcg acctggatcg tgccgcactg ccagtgccgg cccagcgcat ccttgagcgt
     1321 gtactcgatc ttcgggccgt aaaaggcacc ctccccgggc gagatctcga actcgcagcc
     1381 ggagctgcgc aggctggcga tgagcgccgc ctcggcacgg tcccagagct catccgagcc
     1441 gatgcgcttg tccggccggg tggcgacctt gtagagaatg tcggtgaagc cgaagtcggc
     1501 gtagaccttc ttcagcagcg cggtgaaggc gtcgcactcg ggcaggatct ggtcttcggt
     1561 gcagaagatg tggccatcgt cctgcgtgaa gccgcgcacg cgcatgatgc cgtgcaggcc
     1621 gccggtgggc tcgttgcggt ggcaggcacc gaactcgccg tagcgcagcg gcaggtcgcg
     1681 gtagctcttg atgccctgct tgtagatcag aatgtggccc gggcagttca tcggcttgag
     1741 cgcgtagtcg cgcttttccg attcggtggt gaacatgttc tcccggtact tgtcccagtg
     1801 gccggtcttt tcccacaggc ccttgtcgag gatctgcgga cccttgacct cctggtagcc
     1861 gttgtcgcgg tagacacggc gcatgtactg ctcgacctcc tgccagatcg cccagccctt
     1921 ggggtgccag aacaccgtgc cgggcgagtg ttcgtcgagg tggaacaggt cgagttcacg
     1981 cccgagcttg cggtggtcgc gcttctcggc ctcctcgagc atcgtcaggt acttctgcag
     2041 gtcgtccttg gtcgcccagg cggtaccgta gacgcgctgc aacatctcgt tgcggtggtc
     2101 accgcgccag taggcgccgg ccaccttcat cagcttgaag aacttcagct tgccggtgct
     2161 cggcacgtgc gggccacggc acaggtcggt gaagctgcct tcggcgtaga gcgacacgtc
     2221 ctggtcggcc gggatgccct cgatgatctc ggccttgtag gcctcaccga tggacttgaa
     2281 gtaagccacc gcctcgttgc gcggcagcac gctgcgcacg accggctcgt ccttggccgc
     2341 cagctcggcc atcttcttct cgatcgcagc caggtcctcg ggggtgaagg ggcgcttgta
     2401 ggcgaagtcg tagtagaagc cgtgctcgat ggtcggcccg atcgtcacct gcgcgtccgg
     2461 gaacagcgac ttgaccgcat aggccagcag gtgcgcggtc gagtggcgga tgatgtccag
     2521 gccatcggca tccttggcgg tgacgatcgc caactgcgtg tcggcctcga tgcgatggct
     2581 gatgtcgacg aggcgagcct ccgcccccga gccgacgcgg cccgccagcg ccgccttggc
     2641 cagaccggca ccgatcgacg cagccacttc ggccaccgtg acggggccgt cgaactcccg
     2701 gcgcgaaccg tcgggcaact ggatggaaat catggggtgt cctctcgtgg cggcccctgc
     2761 agccgcggat caggcaagaa aaaagcgcgg cgatcgccgc gcttgtctca ggagaaaggc
     2821 aatgagcgag agcggcggtc gaccgactag acctcggcgg aggcggtgaa gggggtgctg
     2881 ctcgttcgcg gtgtcataac cgggctgcct ttctcgtcct tgctcgttga tcgaattcgg
     2941 tattttagtg gcagcaattc gcgatggcgc gacgccgtct cagcgccata cactcagccg
     3001 ccgcagaccc gcgccttcgc agccgcgtac tcggccgcca actgcgccac cagcgccccg
     3061 gccggccgga tcttgtcgat cgcgccgatg ccctggccgc agccccagat gtccttccag
     3121 gcctttttcg catcgccgcc gaaattcatc ttggtcggat cgctttccgg caggtgatcg
     3181 ggatccaggc ccgccgccct gatgcttggc ttcaggtagt tgccatgcac accggtgaac
     3241 aggttgctgt agacgatgtc gtcgctgttg ctgtcgacga tgcact
//
LOCUS       DAOQNN010000371         3261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_20513_length_3261_cov_8.649719, whole genome shotgun sequence.
ACCESSION   DAOQNN010000371 DAOQNN010000000
VERSION     DAOQNN010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20513_length_3261_cov_8.649719"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKV97_14720"
     CDS             <1..670
                     /locus_tag="PKV97_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB07183.1"
                     /translation="DAGALANAAVEPAHCAESPAPAGVLALALDLSRAGEIERARDRL
                     LAAWGGIDLVVFNAGTYRPLRAWELTAEAVRETLETNLVGTMSGTAAVLPTLLAQGHG
                     AIALVGSVAGYGGLPKALVYGPSKAALINFAEVLHLDLSPRGIGVFLIDPGFVATPLT
                     AQNDFAMPALQTPEQAAAAIVDGFARGDFEIHFPKRFTRALKLLQLLPRPLYFALIRR
                     ATGL"
     gene            682..1161
                     /locus_tag="PKV97_14725"
     CDS             682..1161
                     /locus_tag="PKV97_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB07184.1"
                     /translation="MTDPKPPEHLPSSAASAEPVRQSPARRLARFYETLTPAALPGLD
                     QLYAPDARFKDPFNEVAGTAAIRRIFAHMFATTEAPRFEVTDCIEQGEQAMLGWAFHF
                     ALRGRTLTVRGVTHLRFDADGRVVLHRDYWDAAEELYEKLPLIGAPLRWLRRRMSAE"
     gene            1464..2069
                     /locus_tag="PKV97_14730"
     CDS             1464..2069
                     /locus_tag="PKV97_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB07185.1"
                     /translation="MPSNLCAPLRTLLLAVIAPLLLLAGCTPAEPVFKSTDITGTSYG
                     KTLRLTDHNGQERTLADFKGKVVTIFFGYTQCPDVCPTALSGMSTVMQELGPDAERVQ
                     VLFVTVDPERDTPELLAQYVPVFDARFLGLYGTPEKIAELAKEFRVFYRKSGDLAGHY
                     TIDHTAGTYVFGPDGRPRLYVRHAEDPQVVVADIKALLAGK"
     gene            complement(2133..2984)
                     /gene="rpoH"
                     /locus_tag="PKV97_14735"
     CDS             complement(2133..2984)
                     /gene="rpoH"
                     /locus_tag="PKV97_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HNB07186.1"
                     /translation="MSHALTFPVPAAGSLDQYIQSVNRMPMLSEQQEVDLATRLRDEG
                     DVDAARQLVMSHLRVVVAIARGYLGYGLPHADLIQEGNIGLMKAVKRFDPTRGVRLVS
                     FAIHWIKAEIHEYILKNWRLVKIATTKAQRKLFFNLRGMKQDSSTLQPAEVRSIAAQL
                     GVKPEEVVEMETRLTGRDIPLDAGSDDEDERFAPIAYLPDPHAEPSEQVEQAQLARLQ
                     DSGLRDALASLDERSRAIVQRRWLAEGDSATLHELAAEYGVSAERIRQIEAKAMQKMR
                     GMLAAVA"
     gene            complement(3070..>3261)
                     /locus_tag="PKV97_14740"
     CDS             complement(3070..>3261)
                     /locus_tag="PKV97_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNB07187.1"
                     /translation="AICRHYAEQATRLKEAGTPIGANDLWIACHALAEDATVVTHNTR
                     EFRRIAGLRVEDWVSSDED"
BASE COUNT          454 a         1177 c         1089 g          541 t
ORIGIN      
        1 ggacgcgggc gcgctcgcga atgcggccgt cgagcctgcg cactgcgccg agtcgcccgc
       61 tcccgctgga gtgctcgcgc ttgcgctcga cttgtcgcgc gcgggcgaga tcgaacgggc
      121 gcgcgaccgg ctgctcgccg cctggggcgg catcgatctc gtggtcttca acgccggcac
      181 ctaccgcccg ttgcgcgcct gggagctcac tgcggaggcc gtgcgtgaga cgctggagac
      241 caacctcgtc ggcaccatga gcggcaccgc ggcagtcctg ccgaccctgc tcgcgcaggg
      301 tcacggcgcc atcgcgctgg tcggcagcgt cgccggctac ggcggactgc cgaaggcgct
      361 cgtctacggc ccgtccaagg cggcgctgat caacttcgcc gaggtcctcc acctcgacct
      421 gtcgccgcgc ggcatcggcg tcttcctgat cgaccccggc ttcgtcgcca cgccgctgac
      481 cgcgcagaac gacttcgcca tgcccgcgct gcagacgccc gagcaggcgg ccgctgcgat
      541 cgtcgacggc ttcgcgcgcg gcgacttcga gatccacttc ccgaagcgct tcacgcgcgc
      601 gctcaagctc ctgcagctgc tgccgcggcc gctgtatttc gcgctgatcc gccgcgcaac
      661 tggcctgtag ggcttgccgc catgaccgac ccgaagcctc cggagcacct tccatcgagc
      721 gccgcttcgg cggaacccgt acggcaatct cccgcccggc gcctggcgcg cttctacgag
      781 acgctcaccc ccgccgcact gcctgggctc gaccagctct acgcccccga cgcgcgcttc
      841 aaggacccct tcaacgaggt cgccggcacc gccgccatcc gccgcatctt cgcgcacatg
      901 ttcgccacca ccgaggcgcc gcgcttcgag gtcacagact gcatcgagca gggcgaacag
      961 gccatgctgg gctgggcctt ccacttcgcg ctgcgcggcc gcacgctgac ggtgcgcggt
     1021 gtcacgcatc tgcgcttcga cgccgacggc cgcgtggtgc tgcaccgcga ctactgggat
     1081 gccgccgagg agctgtacga gaagctgccg ctgatcggcg ccccgctgcg ctggctgcgg
     1141 cgcaggatgt cggcggagta aggtgtgggg ggaggtgacg ggcgcactgc gatcgcgact
     1201 tgcgtcgctc ccacaggagc cttcgaacac gcggcggttt ttcgtaggag cgacgcaagt
     1261 cgcgatcccc gccgcgcggg gaatccgcgt cggcgcgtgc tccagcgctg cgatcgcgac
     1321 ttgcgtcgct cccacagggg tcttcggaag cgcggcggtt tctcgtggga ccgacgcaag
     1381 tcgcgattcc ccgttgcgga gcgggttccg gcctgctgac gaagggacta tcatcgaccc
     1441 ctgatccgct tgcccgtccc ctcatgccct ccaacctctg cgcgccgctg cgcaccctcc
     1501 tcctcgccgt catcgcgccc cttctcctgc tcgccggctg cacgcccgcc gagccggtgt
     1561 tcaagagcac cgacatcacc ggcacgagct atggcaagac gctgcgcctc accgaccaca
     1621 acggccagga gcgcacgctc gccgacttca agggcaaggt ggtgacgatc ttcttcggct
     1681 acacgcaatg ccccgacgtc tgccccaccg cgctgtcggg catgagcacg gtgatgcagg
     1741 agctcggccc cgacgccgag cgcgtgcagg tgctcttcgt caccgtcgac cccgagcgcg
     1801 acacgcccga gctgctcgcg cagtacgtgc cggtgttcga tgcgcgcttc ctcggcctct
     1861 acggcacacc cgagaagatc gccgagctgg cgaaggaatt ccgcgtgttc taccgcaaga
     1921 gcggtgacct cgccggccac tacaccatcg accacaccgc cggcacctac gtcttcggcc
     1981 ccgacggccg gccgcggctg tacgtgaggc acgccgagga cccgcaggtg gtcgtggcgg
     2041 acatcaaggc cttgctggcg gggaagtgag gacgcgcgct ccaaaggaaa acggcggcct
     2101 cgggccgccg tttcgttgtc tggggccggt ggtcacgcca ccgcggccag catcccgcgc
     2161 atcttctgca tcgccttggc ctcgatctgg cggatgcgct cggccgacac gccgtattcc
     2221 gccgccagct cgtgcagcgt ggcgctgtcg ccctcggcga gccagcgccg ctgcacgatc
     2281 gcgcggctgc gctcgtccag gctggcgagc gcgtcgcgca ggccgctgtc ctgcaggcgg
     2341 gcgagctgag cctgctcgac ctgctcggag ggctcggcgt gcggatcggg caggtaggcg
     2401 atgggtgcga agcgctcgtc ctcgtcgtcg ctgccggcgt cgagcgggat gtcgcggccg
     2461 gtgaggcgcg tctccatctc caccacttcc tcgggcttca cgcccagctg cgcggcgatc
     2521 gagcgcacct cggcgggctg cagcgtgctg ctgtcctgct tcatgccgcg caggttgaag
     2581 aagagcttgc gctgcgcctt ggtggtggcg atcttcacca ggcgccagtt cttcaggatg
     2641 tattcgtgga tctcggcctt gatccagtgg atcgcgaacg acaccagacg cacgccgcgc
     2701 gtggggtcga agcgcttgac cgccttcatc aggccgatgt tgccttcctg gatgaggtcg
     2761 gcatggggca ggccgtagcc gaggtagccg cgcgcgatgg caacgacgac gcgcagatgc
     2821 gacatcacca gttgccgcgc cgcatccacg tcgccctcgt cgcgcagccg cgtcgccaga
     2881 tccacctcct gctgctcgct cagcatcggc atgcgattca cgctctggat gtactgatcc
     2941 aggctgccgg cggcgggaac gggaaaggtc agggcgtgtg acatgggctg gggtcctcct
     3001 tgcgcatcaa tcttagcact cggggcgtga gagtgctaag gggcggcggg agttccgggg
     3061 tagtcctcgt cagtcctcgt cggaggacac ccagtcctcg acgcgcaaac ccgcaatgcg
     3121 cctgaactcg cgcgtattgt gggtgaccac ggtggcgtct tcggccagtg catggcaggc
     3181 gatccacagg tcgtttgcgc cgatcggtgt gcctgcctcc ttgaggcggg tggcctgttc
     3241 cgcgtaatgc cggcagatgg c
//
LOCUS       DAOQNN010000372         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_20582_length_3254_cov_10.323851, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000372 DAOQNN010000000
VERSION     DAOQNN010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20582_length_3254_cov_10.323851"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PKV97_14745"
     CDS             complement(<1..399)
                     /locus_tag="PKV97_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB07188.1"
                     /translation="MKQLVLDIRPDAPPTLENFVAGANAELVATVSLLASPATAAQLP
                     ARHIYLWGAPGSGRSHLLRAAVDAAHATGRPAHLLAAATIEDDLPDLMILDIMLPRLN
                     GLEVLRQLRGERRTEPIILLSARGAETDKVT"
     gene            complement(396..1502)
                     /locus_tag="PKV97_14750"
     CDS             complement(396..1502)
                     /locus_tag="PKV97_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNB07189.1"
                     /translation="MKPPRVDRLQTVAWTATGAALVALLWLLGPILTPFVVGAVFAYI
                     CDPAVNWMVARRVPRALAVLLVILALGLLLIALALILVPMVYREGVLLVRRLPELVQM
                     FNLNVAPLLEARLGVDIKLNAEQFQQLIADNWTSAQELVPAVLAHLKTGGMAVLGFLA
                     NVVLIPLVMFYLLQEWPRILDELERIVPRPWVDGTKRVLGDIDSVMSEFLRGQLSVML
                     LLAVFYSAGLWLAGLNFWLPVGVLTGLLVFIPYVGFGGGLILAIVAALLQAQGWPPLA
                     GVAIVYALGQVVESFVLTPYLVGERIGLHPLAVIFALMAFGQLFGFVGVLVALPVSAA
                     LLVGLREVREAWLASPVYLGTQPRPIIASERERP"
     gene            1644..2711
                     /gene="purM"
                     /locus_tag="PKV97_14755"
     CDS             1644..2711
                     /gene="purM"
                     /locus_tag="PKV97_14755"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNB07190.1"
                     /translation="MADHHPEPLDPQPSLSYRDAGVDIEAGDALVERIKPFAKRTMRP
                     EVLGGIGGFGALFELSKKYKEPVLVSGTDGVGTKLKLAFQLDKHDTVGQDLVAMSVND
                     ILVQGAEPLFFLDYFACGKLDVDTAADVVQGIARGCELAGCALIGGETAEMPGMYPEG
                     EYDLAGFAVGVVEKADIIDGSRIVPGDVVLGLASSGAHSNGYSLIRKIVERAGTDMMA
                     PFHGRPFRDVVMEPTRIYVKPMLELMRAMPGVVKGMAHITGGGITENVPRILREELTA
                     RIDGDTWALPQMFRWLQFAGGVNQDEMYRVFNCGIGMVVIVSAADAEAAEAKLAAAGE
                     TVYRIGVIDTRREGEAQTIID"
     gene            2857..>3254
                     /locus_tag="PKV97_14760"
     CDS             2857..>3254
                     /locus_tag="PKV97_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HNB07191.1"
                     /translation="MGYRLAADAVLLLHLGFIAFAVLGGLLALRWRLAPWLHLPAVAW
                     AAWIEASGGLCPLTPLENRLRVLAGQSGYAGGFIEHYLLPLIYPAALTRELQLALAGF
                     VVLVNLAVYAWVWRRKLRQGSTGACARRARA"
BASE COUNT          511 a         1144 c         1140 g          459 t
ORIGIN      
        1 cgtcaccttg tcggtctcgg cgccgcgcgc ggacagcaag atgatcggct ccgtgcgccg
       61 ctcgccgcgc agctgacgca gcacctcgag gccgttcaat cgtgggagca tgatgtcgag
      121 gatcatgagg tccggcaggt cgtcctcgat ggtcgcggcg gcgagcaggt gggccggccg
      181 gccagtggca tgcgcggcgt cgaccgcggc gcgcagcagg tggctgcgtc cgctgccggg
      241 cgctccccac aggtagatgt ggcgcgcggg cagctgcgcc gcggtggcgg gcgaggccag
      301 cagcgacacg gtggcgacca gctcggcgtt ggcgccggcg acgaagttct cgagcgtggg
      361 cggggcgtcg gggcggatgt cgaggacgag ctgcttcatg ggcgctcgcg ctcgctcgcg
      421 atgatcggcc gcggctgcgt gccgagatac accgggctgg cgagccaggc ctcgcgcacc
      481 tcgcgcaggc ctaccagcag cgcggcgctc accggcagcg cgaccagcac gccgacgaag
      541 ccgaagagct ggccgaaggc catcagcgcg aagatcaccg cgagcgggtg caggccgatg
      601 cgttcgccca ccaggtaggg cgtgagcacg aagctctcga cgacctgacc gagcgcatac
      661 acgattgcca cgccggccag cggcggccag ccctgcgcct gcagcagcgc ggcgacgatg
      721 gcgaggatca gcccgccgcc gaagccgacg taggggatga agaccagcag gccggtgagc
      781 acgcccaccg gcagccagaa gttgagcccg gccagccaca ggccggcgct gtagaacacc
      841 gccagcagca gcatcaccga gagctggccg cgcaggaact cggacatcac cgagtcgatg
      901 tcgccgagga cgcgcttggt gccgtcgacc caggggcgcg gcacgatgcg ctcgagctcg
      961 tcgaggatgc gcggccactc ctgcagcagg tagaacatca ccagcggaat cagcaccacg
     1021 ttggcgagga agccgagcac cgccatgccc ccggtcttga ggtgggcgag caccgccggc
     1081 accagttcct gcgcgctcgt ccagttgtcg gcgatcagct gctggaactg ttcggcgttg
     1141 agcttgatgt ctacgccgag gcgcgcctcg agcagcggcg cgacgttgag gttgaacatc
     1201 tgcaccagct cgggcaggcg gcgcaccagc agcacgccct cgcgatagac catcggtacc
     1261 aggatcagcg cgagcgcgat cagcagcagg ccgagcgcga ggatcaccag cagcaccgcc
     1321 agtgcccgcg gcacgcggcg cgcgaccatc cagttgaccg ccgggtcgca gatgtaggcg
     1381 aacaccgccc ccaccacgaa gggggtgagg atgggcccga gcagccagag cagggcgacg
     1441 agggcggcgc cggtcgcggt ccaggcgacg gtttgcaggc gatcgacgcg gggaggcttc
     1501 atttaaagta aaatcgcggg cttggcgcgg gcgaaaaccg cccgcagaac acgtaaatgt
     1561 agccacttgg cgttgcggcc tcaagctcgg tccctccggg catgcggcga cgcccggcag
     1621 cctgcgagcc cgaaggaacg atcttggccg accatcatcc cgaaccgctg gatccccagc
     1681 cctccctttc ctaccgcgac gccggcgtgg acatcgaagc cggcgacgcg cttgtcgagc
     1741 gcatcaagcc cttcgccaag cgcaccatgc gccccgaggt gctgggcggc atcggcggct
     1801 tcggcgcact cttcgagctg tcgaagaagt acaaggagcc ggtgctggtg tccggcaccg
     1861 acggcgtcgg caccaagctc aagctcgcct tccagctcga caagcacgac accgtcggcc
     1921 aggacctggt ggcgatgagc gtgaacgaca tcctggtgca gggcgccgag ccgctgttct
     1981 tcctcgacta cttcgcctgc ggcaagctcg acgtcgacac cgccgccgac gtggtccagg
     2041 gcatcgcccg cggctgcgag ctcgccggct gcgcgctcat cggcggcgaa accgccgaga
     2101 tgcccggcat gtaccccgag ggcgaatacg acctcgccgg cttcgcggtc ggcgtggtcg
     2161 agaaggccga catcatcgac ggcagccgga tcgtccccgg cgacgtggtg ctcggcctgg
     2221 cctcgagcgg cgcgcactcc aacggctact cgctgatccg caagatcgtc gagcgcgccg
     2281 gcaccgacat gatggcgccc ttccacggcc gtcccttccg cgacgtggtc atggaaccca
     2341 cccgcatcta cgtcaagccc atgctcgagc tcatgcgcgc gatgcccggc gtggtcaagg
     2401 gcatggccca catcaccggc ggcggcatca ccgaaaacgt gccgcgcatc ctgcgcgagg
     2461 agctgaccgc gcgcatcgac ggcgacacct gggcgctgcc gcagatgttc cgctggctgc
     2521 agttcgccgg cggggtgaac caggacgaga tgtatcgcgt gttcaactgc ggcatcggca
     2581 tggtggtgat cgtgtcggcg gccgacgccg aggccgccga ggcgaagctc gccgccgcgg
     2641 gcgagaccgt gtatcgcatc ggcgtcatcg acacccgccg cgaaggcgag gcgcagacca
     2701 tcatcgactg aggatgtgcc ccggcgcgca gggggcgacc cgtgcgcgcc gcgttatcga
     2761 cctcggtcga gccgcgtttc cgcgccgctt tccgcgttcc catccgcgtt cccctccgcg
     2821 ttccgttcca tcccccggcc tggagctcgc cgcgccatgg gctatcgcct cgccgccgac
     2881 gcggtgctgc tgcttcacct gggcttcatc gccttcgccg tgctgggcgg gctgctcgcg
     2941 ctgcgctggc ggcttgcccc ctggctgcat ctgcccgcgg tcgcctgggc cgcgtggatc
     3001 gaagcgagcg gcgggctgtg tccgctcacc ccgctcgaga accgcctgcg cgtgctggcc
     3061 ggccagtccg gctatgcggg cggcttcatc gagcactatc ttcttccgct gatctatccc
     3121 gccgcgctca cccgcgaact gcagctcgcg ctcgccggct tcgtcgtgct cgtcaacctg
     3181 gcggtgtatg cgtgggtgtg gcgtcggaag ctgcgccagg gcagcactgg tgcgtgcgcc
     3241 aggcgggcgc gggc
//
LOCUS       DAOQNN010000373         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_21055_length_3210_cov_11.303328, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000373 DAOQNN010000000
VERSION     DAOQNN010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21055_length_3210_cov_11.303328"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1943)
                     /locus_tag="PKV97_14765"
     CDS             complement(<1..1943)
                     /locus_tag="PKV97_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNB07192.1"
                     /translation="MNTSVSSAVTHRLAFFKGLQTLTNRIHASKDTDEIMLELAPELC
                     SLFEAERFTIYALEEGGASIVTRVKTGLSGVQSIRLPIGENSIAGFVALSGQLVNIRD
                     VYDDEELRRISPHLALRREVDASTGFHSRQMLVAPIRDPDTGSLLGVVQLINTLNREP
                     FSKMTEEGLMGLAQTLGVAFLRHNRQAGRLRSRFEALVVDGRITAEEFADATREARES
                     GAGTENLLIEQLGLSHAELGAAASRYFGVPYQPFVANHVKPMDLLRNIKREFVERSLW
                     LPWEENPEGVVILCVDPEQVRSSGVAQNVLPKKRLTYRVTTQRDFELTVEQFFGVTLD
                     DASVSDLLSGLDDHEEDALSLRDEVNAAADNELVKLVNKIIIDAYRQGASDIHIEPRP
                     GKEKTQIRFRKDGSLVPYIEIPASYRNPLVTRIKIMCDLDISERRKPQDGKIRFRKFA
                     PVDLELRVATLPTQGGMEDVVMRLLAHSEPIPIDQLGLLPFNLERLKSAIAKPYGIFF
                     VCGPTGSGKTTTLHSILSYINTPDTKIWTVEDPVEITQKGLRQVQVNRKAGIDFATMM
                     RAFLRADPDVIMVGEMRDHETVSVGIEASLTGHLVFSTLHTNSAPESVVRLLDMGMDP
                     FNFADALLGVLAQRLAKRLCLKCR"
     gene            complement(2026..>2388)
                     /locus_tag="PKV97_14770"
     CDS             complement(2026..>2388)
                     /locus_tag="PKV97_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNB07193.1"
                     /translation="PARHTVPAVAYRLDSAHGQLVFSGDTAYCPELIEAINDCPQLRH
                     LILETAFAEEQHGLAVASRHLCPSMARAVLDEIRGTPEIHISHLKPGVGQRIIDQLGA
                     CAPGRPLRALGRGDLLEF"
     assembly_gap    2389..2417
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2418..2804)
                     /locus_tag="PKV97_14775"
     CDS             complement(<2418..2804)
                     /locus_tag="PKV97_14775"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNB07194.1"
                     /translation="MKLKVLGCSGGIGGAQPRTTSFLLDDDILIDCGTGVGDLTLDEL
                     ARIDHVFLTHAHLDHIAALPLMIDSVGELRQAPVQVHATPETIRILHSHIFNWLVWPD
                     FSAIPDHHQPFMRFHPIHVGERRDCGG"
     gene            complement(2919..>3210)
                     /locus_tag="PKV97_14780"
     CDS             complement(2919..>3210)
                     /locus_tag="PKV97_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNB07195.1"
                     /translation="ATAASAAGEGQGVIQVLSGANAGRELPLTKSLTTLGKPGRQVAV
                     ITRRPHGYFITHVEGASFPLVNGRAIDAQARPLRDHDIIEIAGVKMEFFLRA"
BASE COUNT          481 a         1001 c         1175 g          524 t
ORIGIN      
        1 cggcacttca ggcacaggcg cttggccagg cgctgggcga gcacgccgag cagcgcgtcg
       61 gcgaagttga acgggtccat gcccatgtcg agcaggcgca cgaccgattc gggcgcgctg
      121 ttggtgtgca gggtcgagaa caccaggtgg ccggtcagcg aggcctcgat gccgaccgag
      181 acggtctcgt ggtcgcgcat ctcgccgacc atgatcacgt cggggtcggc gcgcaggaag
      241 gcgcgcatca tggtggcgaa gtcgatgccg gccttgcggt tgacctgcac ctggcgcagg
      301 cccttctggg tgatctcgac cgggtcttcc accgtccaga tcttggtgtc gggggtgttg
      361 atgtagctca ggatggagtg cagcgtggtg gtcttgcccg agccggtggg gccgcacacg
      421 aagaagatgc cgtagggctt ggcgatcgcg ctcttgaggc gctcgagatt gaagggcagc
      481 aggccgagct ggtcgatcgg gatcggctcg ctgtgcgcga gcaggcgcat caccacgtcc
      541 tccatgccgc cctgggtggg cagcgtggcc acgcgcagct ccaggtcgac cggggcgaac
      601 ttgcggaagc ggatcttgcc gtcctgcggc ttgcggcgct cggagatgtc gaggtcgcac
      661 atgatcttga tgcgggtgac gagcgggttg cggtagcttg ccgggatctc gatgtagggc
      721 accagcgagc cgtccttgcg gaagcggatc tgggtcttct ccttgcccgg gcggggctcg
      781 atgtggatgt cggaggcgcc ctgacgatag gcgtcgatga tgatcttgtt gaccagcttg
      841 acgagctcgt tgtcggcggc ggcgttgacc tcgtcccgga gcgagagggc gtcctcctcg
      901 tgatcgtcga gaccggacag caggtccgag accgaggcgt cgtccagggt cacaccgaag
      961 aactgctcga ccgtcagctc gaagtcgcgc tgggtggtga cgcggtaggt cagccgcttc
     1021 ttcggcagca cgttctgcgc cacgcccgag ctgcgcacct gctcggggtc gacgcacagg
     1081 atcaccacgc cctcggggtt ctcctcccag ggcagccaca ggctgcgctc gacgaactcg
     1141 cgcttgatgt tgcgcagcag gtccatcggc ttgacgtggt tggcgacgaa gggctgatag
     1201 ggcacgccga aatagcgcga ggccgctgcg ccgagttcgg cgtggctgag gccgagctgc
     1261 tcgatcagca ggttctcggt gcccgccccg ctctcgcgcg cctcgcgggt ggcgtcggcg
     1321 aactcctcgg cggtgatacg gccatcgacc accagcgcct cgaagcgcga acgcaggcgc
     1381 ccggcctggc ggttgtggcg caggaaggcg acgccgagcg tctgtgcgag ccccatcaac
     1441 ccctcctcgg tcatcttgga gaagggttcg cggttgaggg tgttgatgag ctgcacgacc
     1501 ccgagcaggc tgccggtgtc gggatcgcgg atgggcgcga ccagcatctg gcgcgagtgg
     1561 aagccggtgc tggcgtcgac ctcgcggcgc agcgcgaggt gcggcgagat gcggcgcagc
     1621 tcctcgtcgt cgtacacgtc gcggatattg accagttggc cgctgagcgc gacgaagccg
     1681 gcgatgctgt tctcgccgat cggcaggcgg atgctctgca ccccgctcag cccggtcttg
     1741 acccgggtga cgatcgaggc cccgccctcc tccagcgcgt agatggtgaa gcgctcggcc
     1801 tcgaacagcg agcacagctc gggcgcgagc tcgagcatga tctcgtcggt gtccttgctc
     1861 gcatggatgc ggttggtcag cgtctgcaag cccttgaaga aggcgagacg gtgcgtgacg
     1921 gcgctggaca cggaggtgtt catgaggatt cctcgggatg gagacgcccg ggggcgagcg
     1981 gtggcatgca aggtagcgga ttccgcgccg cgcccggtac ccgcatcaga actccaggag
     2041 gtcgccgcga ccgagtgcgc gcagcggtcg tccgggcgcg caggccccga gctggtcgat
     2101 gatgcgctgc ccgacgcccg gcttgaggtg gctgatgtgg atctcgggcg tgccgcggat
     2161 ctcgtcgagc accgcgcgcg ccatgctcgg gcagaggtgg cgcgaagcca cggcgagccc
     2221 gtgctgttcc tcggcgaagg cggtctcgag gatgaggtgg cgcagttgcg gacagtcgtt
     2281 gatcgcctcg atcagctcgg gacagtaggc ggtatctccg gaaaacacca gctgcccgtg
     2341 cgcactgtcg agccggtagg ccactgcggg gacggtatgg cgggcaggnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnccc gccgcaatcg cggcgctcgc cgacgtggat cggatggaag
     2461 cgcatgaagg gctggtggtg gtcgggaatc gcggaaaaat cgggccacac cagccagttg
     2521 aagatgtgcg agtgcaggat gcggatggtc tcgggcgtcg catgcacctg caccggcgcc
     2581 tggcgcagtt cgcccaccga gtcgatcatc agcggcagcg cggcgatgtg gtcgaggtgc
     2641 gcatgggtga ggaagacgtg gtcgatccgc gccagctcgt ccagcgtcag gtcgccgacg
     2701 cccgtcccgc agtcgatcag gatgtcgtcg tcgagcagga acgaggtggt gcgcggttgc
     2761 gccccgccga ttccgccgct gcaccccagc accttgagct tcatggcctc cgtcatccgc
     2821 gtccgcggcg gccggaggcc accgtgcaaa cccatgcgaa accttagcac cgaccgcgcg
     2881 ccggccgcgc gggagaatgt cacgccctcc ccggcccctc aggcgcgcag gaagaactcc
     2941 atcttgacgc ccgcgatctc gatgatgtcg tggtcgcgca ggggacgcgc ctgggcgtcg
     3001 atcgccctgc cgttgacgag cggaaaactc gccccctcga cgtgggtgat gaagtagccg
     3061 tgcgggcggc gcgtgatcac cgcgacctgg cgccccggct tgccgagcgt ggtgagcgac
     3121 ttggtgagcg gcagctcgcg cccggcgttg gcgccactga gcacctggat cacaccctgc
     3181 ccctcgcccg ccgcggaggc ggcggtggcg
//
LOCUS       DAOQNN010000374         3206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_21093_length_3206_cov_13.092669, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000374 DAOQNN010000000
VERSION     DAOQNN010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21093_length_3206_cov_13.092669"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PKV97_14785"
     CDS             complement(<1..337)
                     /locus_tag="PKV97_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HNB07196.1"
                     /translation="MSIKVAINGFGRIGRCTLRAIYEQGLQNEFEVVAINASGDLATN
                     AHLLKYDTTHGRFATSVETEGENVIIIDGKKIPFYSTKDPKGVNWGSHGVDVLLECTG
                     AYTTKAKAQA"
     gene            complement(475..2502)
                     /gene="tkt"
                     /locus_tag="PKV97_14790"
     CDS             complement(475..2502)
                     /gene="tkt"
                     /locus_tag="PKV97_14790"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNB07197.1"
                     /translation="MQSTRNVQPPVFNPITGAIRALAMDAVQQANSGHPGAPMGMAEI
                     AEVLWRHHLRHNPANPKWADRDRFVLSNGHGSMLIYALLHLTGYDLPIEELKRFRQLH
                     SKTPGHPEYGYAPGVETTTGPLGQGITNAVGMALAEKILAAEFNRPGHEMVDHHTYVF
                     LGDGCLMEGISHEACSLAGTWGLGKLVAFYDDNNISIDGHVDGWFTDDTPKRFEAYGW
                     QVIRDVQGHDPVAIEAAIQQAKANTAQPTLICCKTIIGAGAPNKQDSHDVHGAPLGAA
                     EIEAARAHIGWNHPPFHIPADVYAAWDARTKGASLEAQWNAAFAAYRAAHPELAAEFV
                     RRMAGELPADWDAHVAAVLAKVVDKAETIATRKASQNAIEAYAPRLPELIGGSADLAG
                     SNLTLWSGAKGVSKTSGGNYVYYGVREFGMAAIANGISLHGGLIPYTATFLMFSEYAR
                     NALRMAALMKVRQIFVFTHDSIGLGEDGPTHQPVEQTATLRLIPNMDVWRPCDTTESA
                     VAWANAIERKDGPTSLCFSRQNLPFQARTSDQVAAIRKGGYVLSEAPASVNGGKPQAV
                     ILATGSEVALAMAAQKTLAEQGVAVRVVSMPSTNVFDRQDAAYKASVLPAGLPRVAVE
                     AGTTDGWYKYVGLEGRVIGIDRFGESAPAGELFKYFGITAEAVVQAVKSVL"
     gene            complement(2935..>3206)
                     /locus_tag="PKV97_14795"
     CDS             complement(2935..>3206)
                     /locus_tag="PKV97_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HNB07198.1"
                     /translation="HINFQRVPVVDTSNPFIARDVPTQDESMVVIRFKDPRGVDFPYL
                     LRMIHDAFMSRANTIVIPGGKLDLAMQLILTPLIWKLMERRRQWV"
BASE COUNT          441 a          970 c         1215 g          580 t
ORIGIN      
        1 gggcctgggc cttggccttg gtggtgtagg cgccggtgca ttcgagcagc acgtccacgc
       61 cgtggctgcc ccagttcacg cccttggggt ccttggtgga gtagaagggg atcttcttgc
      121 cgtcgatgat gatgacgttc tcgccctcgg tctcgaccga ggtggcgaag cggccgtggg
      181 tggtgtcgta cttgagcagg tgggcgttgg tggccagatc gcccgaggcg ttgatggcga
      241 ccacttcgaa ctcgttctgc aggccctgct cgtagatggc gcgcagcgtg cagcgaccga
      301 tacgaccgaa accgttgatg gcgaccttga tgctcatcct tgcttctccc tgagataacg
      361 gtggaaaacc gcgtgatttg tgtttgccga tgccctgccg gtccgtgcgg gaccgtgtcc
      421 tgatgtgcag cgggagcgat ccgtttccgg aaccgctccc gcgtcagggt ctcgtcagag
      481 caccgacttc actgcctgca ccaccgcctc cgcggtgatg ccgaaatact tgaagagctc
      541 gcccgccggg gccgactcgc cgaagcggtc gatgccgatc acgcggcctt cgagcccgac
      601 gtacttgtac cagccgtcgg tcgtgccggc ctcgaccgcg acgcgcggca ggccggcggg
      661 cagcacgctc gccttgtacg cggcgtcctg gcggtcgaag acgttggtcg agggcatgga
      721 caccacgcgc accgcgacac cctgctcggc gagggttttc tgcgcggcca tggcgagcgc
      781 gacctcggag ccggtggcga ggatcaccgc ctgcggcttg ccgccattca cgctggccgg
      841 ggcctccgac agcacatagc cacccttgcg gatcgccgcg acctgatcgg aagtgcgtgc
      901 ctggaaggga aggttctggc gcgagaagca caggctggtc gggccatcct tgcgctcgat
      961 cgcgttcgcc cacgccacgg ccgactcggt ggtgtcgcag ggacgccaca cgtccatgtt
     1021 ggggatcagg cgcagcgtgg cggtctgttc caccggttgg tgggtgggac cgtcctcgcc
     1081 gaggccgatc gaatcgtgcg tgaacacgaa gatctggcgc accttcatca gtgcggccat
     1141 gcgcagggcg ttgcgcgcat actcgctgaa catcaggaag gtggcggtgt aggggatgag
     1201 accgccgtgc aggctgatgc cgttggcgat cgccgccatg ccgaattcgc gcacgccgta
     1261 atagacgtag ttgccgccgc tggtcttgct gacgcccttc gcgcccgacc acagcgtgag
     1321 gttggagccg gcgaggtcgg ccgagccgcc gatgagttcg ggcagcctgg gtgcgtaggc
     1381 ctcgatggcg ttctggctgg ccttgcgggt ggcgatggtc tcggccttgt cgacgacctt
     1441 ggcgagcacc gcggcgacgt gggcgtccca gtcggcgggc agctcgccgg ccatgcggcg
     1501 cacgaactcg gcggcgagct cggggtgggc ggcacggtag gcggcgaagg cggcgttcca
     1561 ttgcgcctcg agcgaggcgc ccttggtgcg cgcgtcccag gccgcgtaga cgtcggccgg
     1621 gatgtggaag ggcgggtggt tccagccgat gtgggcgcgc gcggcctcga tctcggcggc
     1681 accgagcggg gcgccatgca cgtcgtggct gtcctgcttg ttgggcgcgc cggcgccgat
     1741 gatggtcttg cagcagatca gcgtgggctg ggcggtgttg gccttggcct gctggatcgc
     1801 ggcctcgatg gcgaccgggt cgtggccttg cacgtctcgg atcacctgcc agccgtaggc
     1861 ttcgaagcgc ttgggggtgt cgtcggtgaa ccagccgtcg acatggccgt cgatggagat
     1921 gttgttgtcg tcgtagaacg cgaccagctt gcccaggccc caggtgccgg cgagcgagca
     1981 ggcttcgtgc gagatgcctt ccatcaggca gccgtcgccg aggaagacat aggtgtggtg
     2041 gtcgaccatc tcgtggccgg ggcggttgaa ctcggcggcg aggatcttct cggcgagcgc
     2101 catgccgacg gcgttggtga tgccctgacc gagcggaccg gtggtggtct cgacgccggg
     2161 ggcgtagccg tactcggggt ggccgggggt cttgctgtgg agctggcgga agcgcttgag
     2221 ctcctcgatc ggcaggtcgt agccggtgag gtgcagcagc gcgtagatca gcatcgagcc
     2281 gtggccgttg gagagcacga agcggtcgcg gtcggcccac ttggggttgg ccggattgtg
     2341 gcgcaggtgg tggcgccaca gcacctcggc gatctcggcc atgcccatcg gcgcgccggg
     2401 atggccggag ttggcctgct gcaccgcgtc catcgcgagg gcgcggatgg cgccggtgat
     2461 ggggttgaag accgggggct ggacgttgcg ggtcgactgc atgctgtttt cgggccggag
     2521 gccgccatcg ggaaagttcg gaattatccc ttaaaagacc ccggggatgc caatgttgca
     2581 ttgcggcaat gttgcgcggg gcgaggggct ttcgcggggc ggtgggcgcg gcttggaatc
     2641 ggtcacgagg tccgaggagg ggcggcgtcg attcggtcgc ggggtcgggg tggtgtgcgc
     2701 cgcctgcact cgcggccctt cgctgcgctc cgggctgccc tcggcggagg ccgccgcggg
     2761 ggcggcgacg caaactcgtc gctgcgctcc tcaaacatgc gtcgccttgt ttcccccgcg
     2821 acgccctcca cctcggcgct cctgaagttg gcgcacacca ccccgacccc gcgacctcgc
     2881 gcggagtggg gctggggggc tggtcggtgt gctgccgagg aattccgtcg cgcctcaaac
     2941 ccactggcgg cgacgttcca tcagcttcca gatgagcggg gtgaggatca gctgcatggc
     3001 gaggtcgagc ttgccgccgg ggatcacgat ggtgttggcg cggctcatga aggcgtcgtg
     3061 gatcatgcgc agcaggtagg ggaagtcgac gccgcgcggg tccttgaagc ggatcaccac
     3121 catggattcg tcctgggtag ggacgtcgcg tgcgatgaag gggttggagg tgtcgaccac
     3181 cggcacgcgc tggaagttga tgtggg
//
LOCUS       DAOQNN010000375         3205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_21097_length_3205_cov_6.685714, whole genome shotgun sequence.
ACCESSION   DAOQNN010000375 DAOQNN010000000
VERSION     DAOQNN010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21097_length_3205_cov_6.685714"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1019)
                     /locus_tag="PKV97_14800"
     CDS             complement(27..1019)
                     /locus_tag="PKV97_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004386048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB07199.1"
                     /translation="MNANPHLAIIGAGIAGLACARSLADSGVRVTVLDKSRGPGGRTS
                     TRRGEGWACDHGAQYFTARHPDFIEELAAWQAAGVAAPWPARLTVFDSDGRRGVHREE
                     ARFVGTPRMTAPARHLARGLDLRVQLTVSALQRYPHGWEIATAERGMLPEAFDGVLLA
                     VPSPQAVPLLLPLSPALAATAQAARMQACWALMARFDAPLAVDFDAAFVNRGPLRWVA
                     RDRSKPGRDGLETWSLHASAEWSEAHIEDAPERVAVALVGAFEELGGRMPLGWAAHRW
                     RYAITESALDGGCAWDAAQAVGLCGDWANGGRVEGAWLSGRALARRVLDGLAAG"
     gene            1268..1729
                     /locus_tag="PKV97_14805"
     CDS             1268..1729
                     /locus_tag="PKV97_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HNB07200.1"
                     /translation="MNVLLPDSVRRTANRLGYAGLIPFVVLAPASLLDAHHGITWSDA
                     LFAYGAVILSFVCALHWGFAMALPGLDERTRVRSLLWSVVPSLIAWPALLLVPSDAAV
                     LLVLGFVLHYVQDRRLARVAGLPEWYLPLRLRLTTVACIALISGAFVQWPY"
     gene            1783..3084
                     /locus_tag="PKV97_14810"
     CDS             1783..3084
                     /locus_tag="PKV97_14810"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNB07201.1"
                     /translation="MNTAASAIDPSLPVADAARSAAVPGGRLSRLARLGSLATGVAGG
                     MLAEGARQLAAGKRPKVSELVLTPANARRVAEQLAQLRGAAMKVGQLMSMDAGSLLPP
                     ELADILARLREDARTMPMSQVVQVLETHWGKGWEQGFERFSFTPCAAASIGQVHRART
                     RDGEELAIKLQYPGVRRSIDSDVDNVATLLRVSGLLPKALDLAPLLAEAKRQLHEEAD
                     YRREAESLHRFGGLLGDAEHFVLPRAVDALTRSDILAMSWVEGVAVESLAEPQAADQA
                     LRDRIAGLLIGLLFRELFEFRLIQTDPNFANYRFDAASGRLVLLDFGATRPYAEPVVE
                     AYRRLMAGTVRGDRAAMGEAAQAIGYFQDNIHAHQRDAVIDLFEIACEPVRHPGAYDF
                     GTSDLPLRLRDTGLKLSMERDFWHPPPADALFLHRKLGGDA"
BASE COUNT          419 a         1205 c         1113 g          468 t
ORIGIN      
        1 ccggcagcgc attcatgcgc gcccgctcag cccgccgcga ggccgtcgag cacccggcgc
       61 gcgagcgcgc gcccgctcag ccaggcgccc tccacgcggc cgccgttggc ccagtcgccg
      121 cacaggccca cggcctgcgc ggcatcccag gcgcagccgc cgtcgagcgc ggattcggtg
      181 atcgcgtagc gccagcggtg cgccgcccag cccagcggca tgcgcccgcc gagttcctcg
      241 aaggcgccga ccagcgccac ggcgacgcgc tcgggcgcat cctcgatgtg cgcctcgctc
      301 cactccgcgc tcgcgtgcag cgaccaggtc tccagcccgt cccgccccgg cttggagcgg
      361 tcgcgcgcga cccagcgcag cggcccgcga ttgacgaagg cggcgtcgaa atccaccgcc
      421 aggggggcat cgaagcgcgc catcagcgcc cagcaggcct gcatccgcgc cgcctgcgcc
      481 gtcgcggcga gcgccggcga caagggcagc agcagcggca ccgcctgcgg cgagggcacc
      541 gcgagcagca cgccgtcgaa ggcctcgggc agcatcccgc gctcggcggt ggcgatctcc
      601 cagccgtgcg ggtagcgctg cagcgcactc accgtcagct gcacgcgcag gtccaggcct
      661 cgcgccaggt ggcgggcggg cgcggtcatg cgcggcgtgc cgacgaagcg cgcctcctcg
      721 cggtgcacgc cgcggcggcc gtcgctgtcg aacacggtga ggcgcgccgg ccacggcgcc
      781 gccacccccg ccgcctgcca ggccgcgagt tcctcgatga agtccgggtg gcgcgcggtg
      841 aagtattgcg cgccgtggtc gcaggcccag ccctcgccgc ggcgggtgct cgtgcgcccg
      901 cccgggccgc gactcttgtc gaggacagtg acgcggactc cggagtcggc gaggctgcgc
      961 gcgcaggcca ggccggcaat cccggcgccg atgattgcaa ggtgtggatt ggcgttcatg
     1021 ggcgatccgg gaggaagggg ggagttggac acgagcttga atatactctt tttgtcctgg
     1081 acaaaatagg attggcttca taagatgaac tcaagcgcgg ggcgtgcatg gccaagtcgc
     1141 gcaagccgaa tccgagcctc cgttggcggc gcagggccgg cggggccggc cacgacgccc
     1201 accccggact ccgctctgtc cctccgcccc caagcctgcc cttcttcctc ccccgcgagc
     1261 cgcgatcatg aacgtcctgc ttcccgactc cgtccgccgc accgccaacc ggctcggcta
     1321 cgccggcctg atccccttcg tggtgctcgc ccccgccagc ctgctcgacg cccaccatgg
     1381 catcacctgg agcgacgcgc tgttcgccta cggcgccgtg atcctcagtt tcgtgtgcgc
     1441 gctgcactgg gggttcgcga tggcgctgcc cgggttggac gaacgtactc gcgtgcgcag
     1501 cctgctgtgg agcgtggtgc cctcgctgat tgcctggccg gcgctgctgc tggtgccgtc
     1561 ggacgccgcg gtgctgctgg tgctcggctt cgtgttgcac tacgtgcagg accgccgtct
     1621 ggcgcgcgtg gccggtctgc ccgagtggta tctgccgctg cgcctgcgcc tcaccaccgt
     1681 cgcctgcatc gcgctgatca gcggcgcctt cgtgcaatgg ccgtactgaa gtctgcactt
     1741 gcccacccga gcccggcccg aatctccacc gaggagcacg ccatgaacac tgccgccagc
     1801 gccatcgacc cgagccttcc cgtcgcggat gccgcgcgca gcgccgcggt gcccggcggc
     1861 cgcctgtccc gtctcgcccg cctgggcagt ctcgccaccg gggtggcggg cggcatgctc
     1921 gccgagggtg cgcgccagct cgccgccggc aaacgaccca aggtgagcga gctggtgctc
     1981 accccggcca acgcccgccg cgtcgccgaa cagctcgcgc agctgcgcgg tgctgcgatg
     2041 aaggtcggcc agctgatgtc gatggacgcc ggcagcctgc tgccgcccga actcgccgac
     2101 atcctcgccc gcctgcgcga ggacgcgcgc acgatgccga tgagccaggt ggtgcaggtg
     2161 ctggagacgc actggggcaa gggctgggag cagggcttcg agcgcttctc cttcaccccc
     2221 tgcgcggcgg cctcgatcgg ccaggtgcat cgcgcgcgca ctcgcgatgg cgaggaactg
     2281 gcgatcaagc tgcaatatcc cggcgtgcgg cgcagcatcg acagcgacgt ggacaacgtc
     2341 gccaccctgc tgcgcgtctc cgggctgctg cccaaggcgc tcgatctcgc gccgctgctc
     2401 gccgaggcca agcgccagct tcacgaggag gccgactacc gtcgcgaggc cgagagcctg
     2461 caccgcttcg gcggcctgct cggcgatgcg gaacacttcg tgctgccgcg cgcggtcgat
     2521 gcgctcacgc gcagcgacat cctggcgatg agctgggtgg agggcgtggc ggtggagtcg
     2581 ctcgcagagc cgcaggcggc cgaccaggcg ctgcgcgacc gcatcgcggg cctcttgatc
     2641 gggctgctgt tccgcgagct cttcgagttc cgtctcatcc agaccgaccc caacttcgcc
     2701 aactaccgct tcgacgccgc cagcggccga ctggtgctgc tcgacttcgg cgccacccgg
     2761 ccctatgccg agccggtggt cgaggcctac cgtcgcctga tggcgggcac ggtgcgcggc
     2821 gaccgcgccg cgatgggcga ggcggcgcag gcgatcggct acttccagga caatatccat
     2881 gcccatcagc gcgatgcggt gatcgacctc ttcgagatcg cttgcgagcc ggtgcgccac
     2941 cccggcgcct acgatttcgg taccagcgac ctgccgctgc gcctgcgcga taccgggctg
     3001 aagctgagca tggaacgcga cttctggcac cccccgcccg ccgatgcgct cttcctgcac
     3061 cgcaagctcg gcggcgatgc gtagcgtcgc gcttatgagg cgacggactg ctcgcccgcg
     3121 cggctcgtct ggcgctggcg caggatgccc gcgcgctggt gctgtgtcct gcgctgctcg
     3181 tcggtcaggt tgcggtaatc ggcgg
//
LOCUS       DAOQNN010000376         3204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_21115_length_3204_cov_8.244840, whole genome shotgun sequence.
ACCESSION   DAOQNN010000376 DAOQNN010000000
VERSION     DAOQNN010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21115_length_3204_cov_8.244840"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3204
                     /gene="putA"
                     /locus_tag="PKV97_14815"
     CDS             <1..>3204
                     /gene="putA"
                     /locus_tag="PKV97_14815"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HNB07202.1"
                     /translation="SARLPAALREPVRALAHDLVVGLRATRTRSSGVDALMKEFSLSS
                     QEGVALMCLAEALLRVPDKATADRLIRDKLADGDWRAHIGNSPSLFVNAATWGLLITG
                     RLVSTSSEQGLSSALTRLVARGGEPLIRKGMDLAMRMLGEQFVTGRDIDEALERGREH
                     ENKGYRYSFDMLGEAAMTASDAERYFRDYERAIQAIGVASRGRGVVDGNGISVKLSAL
                     HPRYTWSQRERVLAELLPKLKTLCLQARRHDIGLNIDAEEADRLDLSLDLLEALATDD
                     ALAGWTGLGFVVQAYQKRAPHVLDFVIDLARRSGQRMMVRLVKGAYWDAEIKRAQIDG
                     LAGYPVYSRKVYTDVSYLACAKKLLAARDVIYPQFATHNAHSLAAIFHLAAADGRAWQ
                     PGDYEFQCLHGMGEPLYDQIVGRADRHRLVRIYAPVGSHETLLAYLVRRLLENGANTS
                     FVNRIVDERVPVEELIADPVEQAAPLAGAPHPKIALPAELFGGLRTNSAGIDLASEPV
                     RARIAAALANSRTTPFAAGPIVAGRSAVPADVTPVSNPADRGELVGGVAEATTDDLEH
                     ALAAATAAAADWAATPAAARSERLERAAALFEREADTLLALAVREAGKSWANAVAELR
                     EAVDFLRYYAQQARGFDPASHVALGPVLCISPWNFPLAIFSGQVAAALAAGNPVLAKP
                     AEQTPLIAAHAVRLLHEAGIPAAALQLLPGRGETVGAALVADARVRGVMFTGSTEVAA
                     LINRSLAARGGKVPLIAETGGQNAMIVDSTALPEQVVTDVLASAFDSAGQRCSALRVL
                     CLQQDIADDVLHMLQGALAELRLGNPADVRVDIGPVIDAEARDGLEAHVAAMLAKGAR
                     VTRLPLPAACAHGTFVAPSIIEIGALSELGREQFGPILHVLRYRASELDSLIDAIDAS
                     GYGLTMGVHTRIDETVERVAARAHVGNLYVNRNIIGAVVGVQPFGGEGLSGTGPKAGG
                     PLYLHRLLARTPGPVLADGALAVTDSDETRAADAAFSGFLEWLDGAGRGLLEGDELAA
                     LRDRADACRARRIAGLRLALPGPTGEDDSLRFTARGT"
BASE COUNT          438 a         1248 c         1085 g          433 t
ORIGIN      
        1 gtccgcccgc ctccccgcgg ccctgcgcga acccgtgcgc gccctcgccc acgacctcgt
       61 cgtcggcctg cgcgccacgc gcacgcgctc gtccggcgtc gacgccctga tgaaggagtt
      121 ctcgctctcc agccaggaag gcgtggcgct gatgtgcctg gccgaggccc tgctgcgcgt
      181 gcccgacaag gccaccgccg accgcctgat ccgcgacaag ctcgccgacg gcgactggcg
      241 cgcacacatc ggcaacagcc cctcgctgtt cgtcaacgct gccacctggg gcctgctgat
      301 caccggccgg ctggtgtcga ccagcagcga acaaggcctg tcctccgcgc tcacccgcct
      361 ggtggcgcgc ggcggcgagc cgctgatccg caagggcatg gacctggcga tgcgcatgct
      421 cggcgagcag ttcgtcaccg ggcgcgacat cgacgaggcc ctcgagcgcg gccgcgagca
      481 cgagaacaag ggttaccgct actccttcga catgctcggc gaggcggcga tgacggcaag
      541 cgacgcagag cgctatttcc gcgactacga gcgcgccatc caggccatcg gcgtcgcctc
      601 ccggggccgc ggcgtggtcg acggcaacgg catctcggtc aagctctcgg ccctgcatcc
      661 gcgctacacc tggtcgcagc gcgagcgcgt gctggccgag ctgctgccga agttgaagac
      721 cctgtgcctg caggccaggc gccacgacat cgggctgaac atcgacgccg aggaggccga
      781 ccgcctcgac ctctcgctcg acctgctcga ggcgctcgcc accgacgacg cgctcgccgg
      841 ctggacggga ctgggcttcg tggtccaggc ctaccagaag cgcgcccccc acgtgctcga
      901 cttcgtcatc gacctcgcgc gccgcagcgg ccagcgcatg atggtgcgcc tggtcaaggg
      961 cgcgtactgg gacgccgaga tcaagcgcgc gcagatcgac gggctggccg gctacccggt
     1021 gtactcgcgc aaggtgtaca ccgatgtgtc ctacctcgcc tgcgcgaaga agctgctcgc
     1081 cgcacgcgac gtcatctacc cgcagttcgc cacccacaac gcacattcgc tggcggcgat
     1141 cttccacctc gccgccgccg acggccgcgc atggcagccg ggcgactacg agttccagtg
     1201 cctgcacggc atgggcgagc cgctctacga ccagatcgtc ggccgcgccg accgtcaccg
     1261 cctggtgcgc atctacgccc cggtgggcag ccacgagacc ctgctcgcct atctggtgcg
     1321 ccgcctgctc gagaacggcg ccaacaccag cttcgtgaac cgcatcgtcg acgagcgcgt
     1381 gccggtggag gagctgattg ccgacccggt cgagcaggcc gccccgctcg ccggcgcgcc
     1441 gcatccgaag attgcgctgc ccgccgagct gttcggtggc ctgcgcacca actccgccgg
     1501 catcgacctc gccagcgaac cggtgcgcgc ccgaatcgcc gcggcgctgg ccaactcgcg
     1561 caccacgccc ttcgcggccg gtccgatcgt cgccggccgc agcgcggtgc ccgcggacgt
     1621 gaccccggtc tccaacccgg ccgaccgcgg cgagctcgtc ggcggcgtcg ccgaagcgac
     1681 gaccgacgac ctcgagcacg cgctcgccgc cgccactgcg gccgcagccg actgggccgc
     1741 caccccggct gcggcgcgca gcgaacgcct ggagcgcgcc gcggcgctgt tcgaacgcga
     1801 ggccgacacc ctgctcgcgc ttgcagtgcg cgaagccggc aagtcgtggg ccaacgcggt
     1861 ggccgagctg cgcgaggcgg tcgacttcct gcgctactac gcccagcagg cgcgtggctt
     1921 cgaccctgcc agccacgtgg cgctcggtcc ggtgctgtgc atcagcccgt ggaacttccc
     1981 gctggcgatt ttcagcggcc aggtcgccgc cgcgctcgcc gccggcaatc cggtgctcgc
     2041 caagccggcc gagcagaccc cgctgatcgc cgcccatgca gtgcgcctgc tgcacgaggc
     2101 cggcatcccg gctgcggcgc tgcagttgct gcccggccgc ggcgagaccg tcggcgccgc
     2161 gctggtggcc gatgcgcgcg tgcgtggggt gatgttcacc ggctccaccg aggtcgccgc
     2221 gctgatcaac cgcagcctcg ccgcgcgtgg cggcaaggtg ccgctgatcg ccgagaccgg
     2281 cggccagaac gcgatgatcg tggactccac cgcactcccc gagcaggtgg tgacggacgt
     2341 cctcgcctcc gccttcgact ccgccggcca gcgctgctcg gcgctgcgcg tgctgtgcct
     2401 gcagcaggac atcgccgacg acgtcctcca catgctgcag ggcgcgctcg ccgaactgcg
     2461 cctgggcaat ccggcggacg tgcgcgtgga catcggcccg gtcatcgacg ccgaggcgcg
     2521 cgacggtctc gaggcccacg tcgccgcgat gcttgccaag ggcgcccgcg tcacccgcct
     2581 gccgctgccc gccgcatgcg cgcacggcac cttcgtcgcg ccgtcgatca tcgagatcgg
     2641 tgccctgagc gaactgggcc gcgagcagtt cggccccatc ctgcacgtgc tgcgctaccg
     2701 cgcgtccgaa ttggacagcc tgatcgacgc gatcgacgcc agcggctacg gcctcaccat
     2761 gggggtgcac acccgcatcg acgagaccgt ggagcgcgtc gccgcgcgcg cgcacgtcgg
     2821 caacctgtat gtgaaccgca acatcatcgg cgcggtggtg ggcgtgcagc ccttcggcgg
     2881 cgagggcctg tccggcaccg gcccgaaggc gggcggaccg ctctacctgc accggctgct
     2941 tgcgcgtacg cccggcccgg tgctggccga tggcgcgctc gcggtgaccg acagcgacga
     3001 aacccgcgcc gcggatgccg cgttctccgg cttcctggaa tggctggacg gcgccggccg
     3061 cggcctgctc gagggcgacg agctcgccgc gctacgcgac cgcgccgacg cctgccgtgc
     3121 gcgccgtatt gctggcctgc gcctggcgct gcccggcccc accggcgagg acgacagcct
     3181 gcgcttcacc gcgcgcggca ccat
//
LOCUS       DAOQNN010000377         3196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_21200_length_3196_cov_9.562560, whole genome shotgun sequence.
ACCESSION   DAOQNN010000377 DAOQNN010000000
VERSION     DAOQNN010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21200_length_3196_cov_9.562560"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PKV97_14820"
     CDS             complement(<1..969)
                     /locus_tag="PKV97_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB07203.1"
                     /translation="MVDNTMRAAQAAVVESLRRQFSASGQVPDAGLAVPVLRSWARCQ
                     RLGLDMGERVRPPSTTRGDLMVARERSAALLAHASGVMEHLHEQIRSSGSMVLLADAS
                     GMILHGIGDPAFVDRANRVALQAGANWSECERGTNAIGATLIERAPVEVLGAEHYLDC
                     NGVLTCSAAPIFDWRGELLGALDISGDHRSHQPHTLGLARMGVRLVERRLFESEHARH
                     ALLSFHPRPEGVGGLQEGLLAIDADGEIVAADRQARALLGVEERRVAGLGNFSSLFRG
                     GFGTLIGRAARDPAALVELELRSGARIHARLRLSPAWQAGAQSAPAG"
     gene            complement(1156..1536)
                     /locus_tag="PKV97_14825"
     CDS             complement(1156..1536)
                     /locus_tag="PKV97_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HNB07204.1"
                     /translation="MVERVIATPATLALIEQLKAEHGPDLMFHQSGGCCDNSAANCYL
                     PTDLTIGPHDVHLGEIGGVPFYISASQYEYWKHTQLIIDVVDGNGGTFSLEGGTGKAF
                     HTRSRLYDDAEMAAVEAEDRALGR"
     gene            complement(1628..3148)
                     /locus_tag="PKV97_14830"
     CDS             complement(1628..3148)
                     /locus_tag="PKV97_14830"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB07205.1"
                     /translation="MLYALPGQADAKLQFKTRYDNFIGGQWVAPVKGQYFDVVTPITG
                     QKYTQAARSTAEDIELALDAAHAAFPKWGKADATTRSNILLKIADRLEANLELLAYAE
                     TVDNGKPIRETLNADIPLAVDHFRYFAGCLRSQEGGISEIDENTIAYHIHEPLGVVGQ
                     IIPWNFPILMAAWKLAPALGAGNCVVLKPAESTPISILVLMELIADLLPPGVLNIVNG
                     YGREAGMPLATSKRIAKIAFTGSTSTGRVIAQAAANNLIPATLELGGKSPNIFFADVM
                     DKDDAFLDKAIEGLVLFAFNQGEVCTCPSRALIQESIYDRFIERAVKRVAAIKQGSPL
                     DTDTMMGAQASSEQMSKIQSYLQLGKEEGAQVLIGGARAQLDGDLADGYYIQPTLFKG
                     HNKMRIFQEEIFGPVLAVTTFKDEAEALAIANDTLYGLGAGVWSRNGNVAYRMGRAIQ
                     AGRVWTNCYHAYPAHAAFGGYKESGIGRETHKVMLDHYQQTKNLLVSYSENKLGFF"
BASE COUNT          459 a         1052 c         1165 g          520 t
ORIGIN      
        1 gccggccggg gccgactgcg cgccggcctg ccaggccggg ctcagccgca ggcgcgcgtg
       61 gatgcgtgcg cccgaacgca gctcgagctc gaccagcgcg gccggatcgc gcgccgcgcg
      121 cccgatcagc gtgccgaagc cgccccggaa caggctggag aaatttccca ggcccgccac
      181 ccggcgctct tcgaccccca gcagcgcgcg cgcctggcgg tcggcggcga cgatctcgcc
      241 atccgcatcg atcgccagca gaccctcctg caggccgccg acgccctcgg gacgcggatg
      301 gaaggagagc agcgcatggc gcgcgtgttc gctctcgaac aagcggcgct cgaccaggcg
      361 cacccccatg cgcgcgagcc cgagcgtatg gggctggtgg ctgcggtggt cgcccgagat
      421 gtcgagcgcg ccgagcagct cgccgcgcca gtcgaagatc ggcgccgcgc tgcaggtgag
      481 cacgccgttg cagtcgaggt agtgctcggc gccgagcacc tcgaccggcg cacgctcgat
      541 cagggtggcg ccgatcgcgt tggtgccgcg ctcgcattcg ctccagttcg cgcccgcctg
      601 cagcgcgacg cggttggcgc ggtcgacgaa ggcggggtcg ccgatgccgt gcaggatcat
      661 cccgctggcg tcggcgagca gcaccatgct gcccgaggaa cggatctgtt cgtgcaggtg
      721 ctccatgacg ccactggcgt gcgcgagcaa ggccgcgctg cgctcgcgcg ccaccatcag
      781 gtcgccccgc gtggtgctgg gcgggcgcac acgctcgccc atgtccaatc ccaggcgctg
      841 gcagcgcgcc caggagcgca gcaccggcac cgccaggccc gcgtcgggca cctggccgga
      901 ggcggaaaac tgcctgcgca gcgactcgac caccgccgcc tgcgcggcac gcatcgtgtt
      961 atcgaccatc gtctcctctc cacctgcggc cgcggtgcgg cggcgttcgg cacgcctcgc
     1021 ccgggctctt ccatcgtcgt cgaggccgct ccgggggtcg gacagcggcc gtcaaacgac
     1081 tgaagcaagc atcatgccgc aagcacggca cccgaggcgg ccggccatgg agaaaagtac
     1141 gaggatcgcg gaatcctatc gcccgagggc gcgatcctcc gcctccaccg ccgccatctc
     1201 ggcatcgtcg tacaggcgcg agcgggtgtg gaaggccttg ccggtgcccc cctcgagcga
     1261 gaaggtgccg ccgttgccat cgacgacgtc gatgatgagc tgggtgtgct tccagtattc
     1321 gtattgcgac gcgctgatgt agaagggcac gccgccgatc tcgcccaggt gcacgtcgtg
     1381 ggggccgatg gtcaggtcgg tgggcaggta gcagttggcg gcgctgttgt cgcaacagcc
     1441 gccggactga tggaacatca ggtcgggacc gtgctcggcc ttgagctgct cgatcaatgc
     1501 gagtgtcgca ggggtggcga tgacgcgttc gaccatgtcg cgtgcctccg gaacgaaaaa
     1561 gcgggggcgg tcgcccgccc ccgcgaaggt cgcccgcggt gcgggcgagg gagggagaac
     1621 gccgtgatca gaagaagccg agcttgttct cgctgtagct cacgagcagg ttcttcgtct
     1681 gctggtagtg gtcgagcatg accttgtgcg tctcgcggcc gatgcccgac tccttgtagc
     1741 cgccgaaggc cgcgtgcgcc gggtaggcgt ggtagcagtt ggtccacacg cgcccggcct
     1801 ggatggcgcg gcccatgcgg taggcgacgt tgccgttgcg gctccacacg ccggcgccca
     1861 ggccgtacag ggtgtcgttg gcgatcgcca gggcctcggc ctcgtccttg aaggtggtca
     1921 ccgcgagcac cggcccgaag atctcctcct ggaagatgcg catcttgttg tggcccttga
     1981 acagcgtcgg ctggatgtaa tagccgtcgg ccaggtcgcc gtcgagctgg gcgcgcgcgc
     2041 cgccgatcag cacctgggcg ccctcttcct tgccgagctg caggtaggac tggatcttgc
     2101 tcatctgctc ggaggaggcc tgcgcgccca tcatggtgtc ggtgtcgagc gggctgccct
     2161 gcttgatcgc ggcgacgcgc ttcaccgcgc gctcgatgaa gcggtcgtag atcgactcct
     2221 ggatcagcgc gcggctcggg caggtgcaca cctcgccctg gttgaaggcg aacagcacca
     2281 ggccctcgat cgccttgtcg aggaaggcgt cgtccttgtc catgacgtcg gcgaagaaga
     2341 tgttgggcga cttgccgccc agctcgagcg tggccgggat caggttgttg gcggcagcct
     2401 gggcgatcac gcggccggtg gaggtggagc cggtgaaggc gatcttggcg atgcgcttgc
     2461 tggtggcgag cggcatgccg gcctcgcggc cgtagccgtt gacgatgttg agcacgcccg
     2521 gcggcagcag gtcggcgatc agctccatca gcaccaggat cgagatcggg gtcgactcgg
     2581 cgggcttgag caccacgcag ttgcccgcgc ccagcgccgg cgccagcttc cacgcggcca
     2641 tcaggatcgg gaagttccac gggatgatct ggccgaccac gcccagcggc tcgtggatgt
     2701 ggtaggcgat ggtgttctcg tcgatctccg agatgccgcc ttcctgcgag cgcaggcagc
     2761 cggcgaagta gcggaagtgg tcgacggcga gcgggatgtc ggcgttgagc gtctcgcgga
     2821 tcggcttgcc gttatcgacg gtctcggcgt aggcgagcag ctcgaggttg gcctcgaggc
     2881 gatcggcgat cttgagcagg atgttggagc gcgtggtggc gtcggccttg ccccacttcg
     2941 ggaaggcggc gtgcgcggcg tcgagcgcga gctcgatgtc ctcggcggtg gagcgcgcgg
     3001 cctgggtgta cttctggccg gtgatcggcg tgaccacgtc gaaatactgg cccttgaccg
     3061 gagccaccca ctggccaccg atgaagttgt cgtagcgggt cttgaactgg agcttggcgt
     3121 cggcctggcc gggcagtgcg tacagcatgg tgtcgtctcc tcgtgtggtt tcggttgcgg
     3181 gcggtttcgc cacacg
//
LOCUS       DAOQNN010000378         3192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_21241_length_3192_cov_10.041760, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000378 DAOQNN010000000
VERSION     DAOQNN010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21241_length_3192_cov_10.041760"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..124
                     /locus_tag="PKV97_14835"
     CDS             <1..124
                     /locus_tag="PKV97_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNB07206.1"
                     /translation="YLLDAHHWLILHGRYVCTARKPKCGECGVRDLCLCRDKTD"
     gene            222..656
                     /locus_tag="PKV97_14840"
     CDS             222..656
                     /locus_tag="PKV97_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HNB07207.1"
                     /translation="MFNPSRDQVRSFFIDTWRKARAKEVLTPMETIAADIIALHPEYH
                     AVVEDPEAVHRDFPPEAGQINPFLHLSLHLAIEEQLSIDQPPGIRAAFEAACQHRGDR
                     HDAMHDALECLGEMLFDAQRKGTPPDGAAYVGALRKRAGLAL"
     gene            851..1978
                     /locus_tag="PKV97_14845"
     CDS             851..1978
                     /locus_tag="PKV97_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB07208.1"
                     /translation="MPGLLTHVDPDGLLEYSVVYTDRSLNHMSQAFQGVMRELSATLK
                     KVYHAHAVAIVPGSGTFGMEAVARQFATGRKTLVIRNGWFSYRWTQIFEMGDIPAESV
                     VLKARQVRDEAQAPFVPAPIDEVVAAIRQHRPDVVFAPHVETSAGMMLPDDYLRAVAD
                     ATHAVGGLFVLDCIASGTIWVDMDATGVDVLVSAPQKGWSASPCCAMVMLSAAARERI
                     EGTTSTSFACDLKKWLQIMEAYEGGAHAYHATMPTDGLVTLRNVMRETEAYGFDKVKA
                     EQVELGAGVRALLEARGFPSVAAAGFQAPGVVVSFTTDDGIKTGAKFAAAGLQVAAGV
                     PLACDERPDYKSFRVGLFGLDKLHNVARTVATLEKALDQVG"
     gene            complement(2095..>3192)
                     /gene="hflX"
                     /locus_tag="PKV97_14850"
     CDS             complement(2095..>3192)
                     /gene="hflX"
                     /locus_tag="PKV97_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNB07209.1"
                     /translation="APTDTVPRTGAIEALLVDHEISPSQARNLELETGCEVMDRTMVI
                     LEIFHRNARSRAAKAQVEIARLGYMAPRLREAAKLAGPQGRQRSGVGGRGAGESHTEL
                     DRRKIRDRIAELQLEIVAMEAERKTQRARRQGRQSPASVALVGYTNAGKSTLMRALTG
                     SEVLVANKLFATLDTTVRTLYPESVPRVLVSDTVGFIKNLPHGLVASFKSTLDEALDA
                     GLLLHVIDASDPGFERQLEVTDAVLQEIGADELPRIRVFNKIDHVGDAAAQAECEAAL
                     RAKYPGCIVMSARRPDEVARLRQTIVAFFQQDLVEAELLLPWSAQQLRKEIYANCEVL
                     EERAEDEGAVFRLRGEPDTVERLRAQCALTR"
BASE COUNT          468 a         1202 c         1052 g          470 t
ORIGIN      
        1 ctacctgctc gacgcccacc actggctgat cctgcatggc cgctacgtgt gcaccgcgcg
       61 caagcccaag tgcggcgagt gcggcgtgcg cgacctgtgc ctgtgccgcg acaagactga
      121 ctgaaacccg acgcggacgc ccgccggcgc gcgctgcgcc cgcaccgcac ccgcgcccga
      181 acaccccgcg cccgagtgcg caagagaacc gaggacccga catgttcaac cccagccgcg
      241 accaggtgcg cagcttcttc atcgacacct ggcgcaaggc gcgcgccaag gaagtgctga
      301 cgccgatgga gaccatcgcc gccgacatca tcgcgctcca ccccgagtac cacgccgtgg
      361 tcgaggaccc ggaggcggtg caccgcgact ttccgcccga ggccgggcag atcaacccct
      421 tcctgcacct gtcgctgcac ctggccatcg aggagcagct ctcgatcgac cagccgcccg
      481 gcatccgcgc cgccttcgag gccgcctgcc agcaccgcgg cgaccgccac gacgccatgc
      541 acgacgcgct cgagtgcctc ggcgagatgc tcttcgacgc ccagcgcaag ggcaccccgc
      601 ccgacggcgc ggcctacgtc ggcgcgctgc gcaagcgcgc cgggctggcg ctctgaacgc
      661 agcccgatcg gacgccgttc gccctccgcg cctcgccctg cacgcgggcg aggcgctaga
      721 atgatcgctt tcggacgaat ggatcgggcc cgcctcgacg aggtccgggg ccgatcccgg
      781 tccacgcccc gcccaactca ttcccgcccc ccttcttcct cgaagaatcc ccgctcccca
      841 ggaggtctcc atgcccggtc tgctcaccca cgtcgatccc gatggcctgc tcgaatactc
      901 ggtggtctat accgaccgct cgctcaacca catgtcgcag gccttccagg gcgtcatgcg
      961 cgagctctcg gcgacgctga agaaggtcta ccacgcccac gccgtcgcca tcgtccccgg
     1021 cagcggcacc ttcggcatgg aagcggtcgc gcgccagttc gccaccggcc ggaagaccct
     1081 ggtgatccgc aacggctggt tcagctaccg gtggacgcag atcttcgaga tgggcgacat
     1141 ccccgccgaa tccgtggtgc tcaaggcccg ccaggtgcgc gacgaggcgc aggcgccctt
     1201 cgtcccggcg ccgatcgacg aggtcgttgc ggcgatccgc cagcaccgcc ccgacgtggt
     1261 gttcgccccg cacgtcgaga cctcggccgg catgatgctt cccgacgact acctgcgcgc
     1321 ggtggccgac gccacccacg cggtcggcgg cctcttcgtg ctcgactgca tcgcctcggg
     1381 cacgatctgg gtggatatgg acgccaccgg cgtcgacgtg ctggtgagcg cgccgcagaa
     1441 gggctggagc gcctcgccgt gctgcgcgat ggtcatgctg agcgccgccg cgcgcgagcg
     1501 catcgagggc accaccagca ccagcttcgc ctgcgacctc aagaagtggc tgcagatcat
     1561 ggaagcctac gagggcggcg cccacgccta ccacgccacc atgcccaccg atggcctggt
     1621 cacgctgcgc aacgtgatgc gcgagaccga ggcctacggc ttcgacaagg tcaaggccga
     1681 gcaggtggaa ctcggcgctg gcgtgcgtgc gctgctcgag gcgcgcggct tcccgagcgt
     1741 ggccgccgcg ggcttccagg ccccgggcgt ggtggtgagc ttcaccaccg acgacggcat
     1801 caagaccggc gccaagttcg ccgccgccgg cctgcaggtc gccgccggcg tgccgctcgc
     1861 ctgcgacgag cgtcccgact acaagagctt ccgcgtcggc ctgttcgggc tcgacaagct
     1921 gcacaacgtc gcgcgcaccg tcgccacgct ggagaaggcg ctcgatcagg tcggctgagc
     1981 gggctcgccc cacccgaccg gggggccact cgcccgagac ccgacgccgc atgcgcgcaa
     2041 atccgggcgc atcagctcac cggcaaacgc cacccttgcg gtggcgtttg cgcatcagcg
     2101 tgtcagggca cattgcgcgc gcagtcgttc gacggtgtcg ggctcgccgc gcagcctgaa
     2161 gaccgcccct tcatcctccg ccctttcctc cagcacctcg cagttggcgt agatctcctt
     2221 gcgcagctgc tgcgccgacc acggcagcag cagctccgcc tcgaccaggt cctgctggaa
     2281 gaacgccacg atcgtctgcc gcagcctggc cacctcatcc ggccggcgcg cgctcatgac
     2341 gatgcagccg gggtatttcg cgcgcagcgc ggcctcgcat tcggcctgcg ccgcagcgtc
     2401 gccgacgtgg tcgatcttgt tgaagacgcg gatgcgcggc agctcgtcgg cgccgatttc
     2461 ctgcagcacc gcgtcggtga cctcgagctg gcgctcgaag ccgggatcgc tggcgtcgat
     2521 gacgtgcagc agcaggccgg cgtccagcgc ttcgtccagg gtggacttga acgacgccac
     2581 caggccgtgc ggcaggttct tgatgaagcc gacggtgtcg ctgaccagca cgcgcggcac
     2641 gctctcgggg tagagcgtgc gtacggtggt gtcgagggtg gcgaagagct tgttggcgac
     2701 cagcacctcg ctgcccgtga gcgcgcgcat cagcgtggat ttgcccgcat tggtgtagcc
     2761 gaccagcgcc acgctggccg ggctctggcg accctggcgg cgcgcgcgct gggtcttgcg
     2821 ctcggcctcc atcgcgacga tctccagttg cagctcggcg atgcggtcgc gaatcttgcg
     2881 gcgatcgagc tcggtatgcg actcgcccgc accgcgaccg cccacgccgc tgcgctgccg
     2941 tccctgcggc ccggcgagct tggcggcttc gcgcaggcgc ggcgccatgt agcccaggcg
     3001 ggcgatctcc acctgcgcct tggccgcgcg cgagcgcgcg ttgcggtgga agatctcgag
     3061 gatgaccatg gtccggtcca tcacctcgca gccggtctcg agctcgaggt tgcgcgcctg
     3121 cgaaggcgag atctcgtggt cgaccagcag cgcctcgatc gcgccggtgc gcggcacggt
     3181 gtccgtcggc gc
//
LOCUS       DAOQNN010000379         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_21272_length_3189_cov_11.920549, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000379 DAOQNN010000000
VERSION     DAOQNN010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21272_length_3189_cov_11.920549"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PKV97_14855"
     CDS             <1..897
                     /locus_tag="PKV97_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HNB07210.1"
                     /translation="PAAVPARAAGADAAIPSAQQTVLKAAAPASMQPGSVPAAQQAVA
                     PAPASAPPRRSAREEHAVQPARAAPASVQDVPPWEDLPPEAYADSDLPPPREARNGHD
                     EAYAASAGAHTGAISRARPEPRGGGARRAEEVPSRSAPLPAVRAPEPVTSPAAPTQDA
                     PAASGDAAALLALGDWRGLIRALGLGGLVRELAQHCEWVECGDGVLQLRLSTAHRHLL
                     DMNRGAVERLQDVLCAALGRTLAVRIAIGDISGQTPAQRDAIERQVRHAEAVAALEAD
                     PFVRELIERFDATLVENTVKPL"
     gene            971..1294
                     /locus_tag="PKV97_14860"
     CDS             971..1294
                     /locus_tag="PKV97_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNB07211.1"
                     /translation="MMKGGIAGLMKQAQQMQENMKKMQDQLGSIEVEGQSGAGMVKVQ
                     MTCKYDVRRVSIDESVMDDREMLEDLVAAALNDAVRKVEAATQEKMSSVTAGLNLPPG
                     MKLPF"
     gene            1306..1899
                     /gene="recR"
                     /locus_tag="PKV97_14865"
     CDS             1306..1899
                     /gene="recR"
                     /locus_tag="PKV97_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNB07212.1"
                     /translation="MSPSSLDELIEALRCLPGVGPKSAQRMAYHLLQRDQRGAARLGN
                     ALLHALEVLRHCARCNSFTEDEVCPRCANPRRDAALLCVVEMPADLAMIEQTQSYNGL
                     YYVLMGRLSPLDGVGPRELRLDKLIARATEEGVREVILATNFTNEGEATAHTVASLLG
                     ARGVKVSRLSRGVPVGGELEHTDIGTIGQALIERRAV"
     gene            2085..2678
                     /gene="petA"
                     /locus_tag="PKV97_14870"
     CDS             2085..2678
                     /gene="petA"
                     /locus_tag="PKV97_14870"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNB07213.1"
                     /translation="MSVDQKMDSSRRTLLLATSAAGGVAAVATAVPFVASLTPSERAK
                     AAGAPVEADVSKLKPGEMMTVEWRGKPVWILRRTPEMLASLDKTDEKVSDPNSVKPQQ
                     PEYAKNKHRSIKPEYLVTVGICTHLGCSPSEKFKVGADSGMGADWPGGFLCPCHGSIF
                     DLAGRVYSSMPAPDNLEVPPHQYLADTKILVGDDGKA"
     gene            2682..>3189
                     /locus_tag="PKV97_14875"
     CDS             2682..>3189
                     /locus_tag="PKV97_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNB07214.1"
                     /translation="MTTKSQAVLNWIDERFPLTSTWKAHLSEYYAPKNFNFWYFFGSL
                     ALLVLVIQILTGIFLVMHYKPDASLNASGVPVAFASVEYIMREVPGGWLIRYLHSTGA
                     SAFFIVVYLHMFRGLLYGSYRKPRELIWVFGTLIFLVLMAEAFMGYLLPWGQMSFWGA
                     QVIVNLFSA"
BASE COUNT          492 a         1098 c         1081 g          518 t
ORIGIN      
        1 ccggcggccg tccccgcgcg ggcggccggc gccgatgcgg cgatccccag cgcgcaacag
       61 accgtcctca aggctgcggc gcccgcatcc atgcagcccg gctccgttcc ggcggcgcag
      121 caggccgtcg cacctgcgcc tgcgagcgcg ccgccgcgca gatccgcgcg cgaggagcac
      181 gccgtgcagc cggcgcgtgc tgccccggca agcgtgcagg acgttccgcc ctgggaggat
      241 ctcccgcccg aggcctatgc cgattccgat ctgccgccgc cgcgcgaggc gcgcaacggc
      301 cacgacgagg cctacgccgc ctccgcgggt gcgcacacgg gggcgatctc acgggcacgc
      361 ccggagccgc gtggcggtgg cgcgcggcgg gcggaggaag tcccgtcgcg gtccgcgccc
      421 cttcccgcgg tgcgcgcgcc cgagccggtg acgtctccgg ctgcgccgac ccaggatgcg
      481 cccgccgcca gtggcgatgc cgcggcgctg cttgcgctcg gcgactggcg cggcctgatc
      541 cgcgcgctcg ggctcggcgg actggtgcgc gagcttgccc agcactgcga atgggtcgag
      601 tgtggcgatg gcgtgctgca gctgcgcctg tcgaccgcgc accgccacct gctcgacatg
      661 aatcgcggcg cggtcgagcg cctgcaggac gtgctgtgcg ccgcgctcgg acgcacgctc
      721 gcggtgcgca tcgcgatcgg cgacatctcc ggacagaccc ccgcgcagcg cgacgcgatc
      781 gagcgccagg tccgccatgc cgaggcggtc gcagcgctcg aggccgaccc cttcgtgcgc
      841 gagctgatcg aacgtttcga cgccacgctc gtcgagaaca cggtgaaacc cctttgaacc
      901 ccttcgatgc cgggaggcgc gccctcccga tcgaccccac ccgacaaccg atccgacgga
      961 gaacaccatc atgatgaaag gcggtatcgc gggcctgatg aagcaggccc agcagatgca
     1021 ggaaaacatg aagaagatgc aggaccagct cggctcgatc gaggtcgagg ggcagtccgg
     1081 cgccggcatg gtcaaggtgc agatgacctg caagtacgac gtgcgccgcg tgtccatcga
     1141 cgaatcggtc atggacgacc gcgagatgct cgaggacctc gtcgccgcag cgctcaacga
     1201 cgccgtgcgc aaggtcgagg cggccaccca ggagaagatg tccagcgtca ccgccgggct
     1261 gaacctgccg ccgggcatga agctgccgtt ctgagcggcg cgccgatgtc gccttccagc
     1321 ctcgacgaac tgatcgaggc actgcgctgc ctgcccgggg tcggccccaa gtcggcgcag
     1381 cgcatggcct atcatctgct gcagcgtgat cagcgcggcg cggcgcggct gggcaatgcc
     1441 ctgctgcatg cgctcgaggt gctgcgccat tgcgcgcgct gcaacagctt caccgaggac
     1501 gaggtgtgtc cgcgctgcgc caatccgcgc cgcgacgccg cgctgctgtg cgtggtggag
     1561 atgccggccg atctcgccat gatcgagcag acgcagtcct acaacggcct ctactacgtg
     1621 ctgatgggcc ggctttcgcc gctcgatggc gtcgggccgc gcgaactgcg cctggacaag
     1681 ctgatcgcgc gcgccaccga ggagggggtg cgcgaggtca tcctcgccac caacttcacc
     1741 aacgagggcg aggccaccgc gcacaccgtc gcgagcctgc tcggcgcgcg cggggtcaag
     1801 gtcagccggc tgtcgcgcgg cgtcccggtc ggcggcgagc tcgagcatac cgacatcggc
     1861 accatcggcc aggctctgat cgagcgccgc gcggtctgaa tttccgttgc tgcaatgcag
     1921 caacgcatgc cccgggcggt tcctgatcgg tgtcggtttt cgtgccgaag gccttgttcc
     1981 ggctggcgat cggcccgtcg cgctttctct gctgcagcgt cttcgatata atcggatggt
     2041 tatttgtatc caggtctttc gttttccttt ttgggaagag gctcatgagc gttgatcaga
     2101 aaatggacag cagccgccgc accctgctgc tggcgacgtc cgccgcaggt ggcgtcgccg
     2161 cagtggcgac cgcggtgccg ttcgtcgcca gcctgacgcc gtccgaacgc gcgaaggcgg
     2221 ccggtgcgcc ggtggaggcg gacgtcagca agctgaagcc gggcgagatg atgacggtgg
     2281 agtggcgtgg caagccggtg tggatcctgc gccgcacgcc cgagatgctc gcctcgctcg
     2341 acaagacgga cgaaaaggtc agcgacccca actccgtgaa gccgcagcag cccgagtacg
     2401 ccaagaacaa gcaccgctcg atcaagccgg agtacctggt cacggtcggc atctgcaccc
     2461 acctcggctg ctcgccctcc gagaagttca aggtgggcgc ggacagcggc atgggcgccg
     2521 actggccggg tggcttcctg tgcccctgtc acggctcgat cttcgatctg gccggccgcg
     2581 tgtatagcag catgcccgcg ccggacaatc tcgaggtgcc gccgcaccag tatctcgcgg
     2641 acaccaaaat ccttgttggc gatgacggga aggcataagc gatgacgacc aaatcccaag
     2701 ccgttctcaa ctggatcgac gagcgcttcc cgctcacgtc gacctggaag gcgcacctgt
     2761 ccgagtacta cgcgccgaag aacttcaact tctggtactt cttcggctcg ctggccctgc
     2821 tggtgctggt gatccagatc ctcaccggca tcttcctggt gatgcactac aagccggacg
     2881 cctcgctcaa cgcgtcgggc gttccggtgg cgttcgccag cgtcgagtac atcatgcgcg
     2941 aggtgccggg cggctggctg atccgctacc tgcactcgac cggtgcgtcg gccttcttca
     3001 tcgtcgtgta tctgcacatg ttccgcggtc tgctctatgg ctcttatcgc aagccgcgcg
     3061 agttgatctg ggtcttcggc acgctgatct tcctggtgtt gatggccgag gcgttcatgg
     3121 gctatctgct gccgtggggc cagatgtcgt tctggggcgc ccaggtgatc gtgaacctgt
     3181 tctcggcga
//
LOCUS       DAOQNN010000380         3185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_21318_length_3185_cov_9.554952, whole genome shotgun sequence.
ACCESSION   DAOQNN010000380 DAOQNN010000000
VERSION     DAOQNN010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21318_length_3185_cov_9.554952"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3185
                     /locus_tag="PKV97_14880"
     CDS             <1..>3185
                     /locus_tag="PKV97_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNB07215.1"
                     /translation="TSRSTVGTMTELADHFKLLYARAAHLHCRCCGKPVRRDSAVSIA
                     ADLRARAAAAGDPRLVVCFPVTVPANFSEEEVTGLLNAQGYTRIQERIAGAGAEGQDV
                     LQVVQDRFRASNAEDSRLAEALEAALQRGHGRLAVYASGEGVKAEKSAGTPGEAVWRY
                     SSGLHCADCDIHYSEATPGLFSFNSPIGACETCRGFGRVIGVDFGLVVPDESKTLAEG
                     AIKPWQTESYRECQQDLEKMAKKYGVAMDIPVRDLPPEHRQWLFEGDPKWKNWDSSWP
                     RYWYGVRHFFDWLETKAYKMHIRVLLSRYRSYTECPACHGARLKPDALLWRLPTRAQA
                     SCRSGASRDHHVSSSVGAAEPPIAACAAPTGGAPTVGRGLTIGGAPTVGEGPTMGAAP
                     AVGEAAAAGGIAIAGVQGLAIHELMALPVDRIRDAIAALALPGVADEATELVLGEIRS
                     RLTYLADVGLGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRD
                     IGRILGVMTRLRDAGNTLVVVEHDPQVMVAADELLEIGPGPGERGGDIVARGTPAEIA
                     ANPDSVTGPWLAGRKRIDVDRPPRPVDAATPRLKLVGARQHNLRDLTVGFPLRRLTCL
                     TGVSGSGKSTLIQDVLFPALAKHFGEAAESPGVFDRLENVDNLRGVVMVDQSPIGKSS
                     RSNPVSYVGAWDPIRALFAALPEAKQRGYTPGTFSFNAGHGRCPTCTGSGFEHVEMQF
                     LSDVWLRCPDCDGKRYRPEVLELQWNGLSVADVLELTVHEALDVFREHPKVLAALAPL
                     ADVGLDYLRLGQPVPTLSGGEAQRLKLAGHLAEAAQKKPVRKASIVDKFGDKAGDKTE
                     PGLLFLFDEPTTGLHFEDVAKLLGAFDKLLQAGHSLIVIEHNLDVIAASDWIIDLGPE
                     GGEGGGAIVVEGPPEAVRTHASSHTGAALRDYAEALRKVGDGGRSSADAEAPGSRRSG
                     ASRDHGASGSAVATESPIAACAAPTGDRALVAAEPRVEYRPVVAPAIEIRHAREHNLK
                     NVSLQIPRDQFTVITGLSGSGKSTLAFDIVFG"
BASE COUNT          475 a         1091 c         1134 g          485 t
ORIGIN      
        1 acctcgcgct cgacggtggg cacgatgacc gagctcgccg accacttcaa gctgctctac
       61 gcgcgcgccg cgcatctgca ctgtcgttgc tgcggcaagc cggtgcggcg ggattcggcg
      121 gtgagcatcg ccgccgacct gcgcgcgcgc gccgcggcag cgggcgatcc gcgcctggtg
      181 gtgtgctttc cggtgacggt gccggcgaat ttcagtgagg aggaggtcac cggcctgctc
      241 aacgcgcagg gctatacccg catccaggaa cgcatcgccg gtgccggcgc cgaggggcag
      301 gacgtgctgc aggtggtgca ggaccgcttc cgcgcgtcga acgccgagga ttcgcgtctg
      361 gcggaggcgc tggaggcggc gctgcagcgc ggacacgggc ggcttgccgt ctacgcttcg
      421 ggcgaggggg tgaaggcaga gaagtcggct gggacgccgg gcgaggcggt gtggcgctat
      481 tcctccggcc tgcattgcgc cgactgcgac atccactatt cggaagccac gcccggcctg
      541 ttctcgttca actcgccgat cggcgcctgc gagacctgtc gcggcttcgg acgggtgatc
      601 ggggtggatt tcggcctggt ggtgccggac gaatcgaaga cgctggccga gggcgcgatc
      661 aagccctggc agaccgagag ctatcgcgag tgccagcagg acctcgagaa gatggcgaag
      721 aagtacggcg tggcgatgga catcccggtg cgggatctgc cgccggagca tcgccagtgg
      781 ctgttcgagg gcgacccgaa atggaagaac tgggacagct cgtggccgcg ctactggtac
      841 ggcgtgcggc atttcttcga ctggctggag accaaggctt acaagatgca catccgcgtg
      901 ctgctgtcgc gctaccgcag ctacacggaa tgcccggcgt gccatggggc gcggttgaaa
      961 cccgacgcct tgttgtggcg cttgccgacc cgcgctcagg cttcatgtag gagcggcgca
     1021 agccgcgatc accacgtttc atcgagtgtt ggcgccgctg aacccccgat cgcggcttgc
     1081 gccgctccca cagggggtgc gccgactgtc ggccggggac tgaccatcgg gggggcgccg
     1141 accgtcggcg agggaccgac catgggggct gcgcccgccg tcggcgaggc agccgcggcg
     1201 ggggggattg cgatcgcggg ggtgcagggc ctcgccatcc acgagctgat ggctctgccg
     1261 gtcgatcgca tccgcgacgc catcgccgcg ctcgccctgc ccggcgtggc ggacgaagcc
     1321 accgagctgg tgctcggcga gatccgcagc cgactcacct acctggccga cgtcggcctc
     1381 ggctacctga cgctggaccg ccagtcgcgc acgctgtcgg gcggcgaggt gcagcgcatc
     1441 aacctgacca ctgcgctcgg cacctcgctg gtgaataccc tgttcgtgct cgacgagccc
     1501 tcgatcggcc tgcatccgcg cgacatcggc cgcatcctcg gggtgatgac gcggctgcgc
     1561 gacgccggca acacgctggt ggtggtcgag cacgacccgc aggtgatggt cgccgccgac
     1621 gagctgctcg agatcggccc cggcccgggc gagcgcggcg gcgacatcgt cgcgcgcggc
     1681 acgccggccg agatcgcggc gaatcccgat tctgtgaccg gcccctggct ggcgggcagg
     1741 aagcgcatcg acgtcgaccg tccgccgcgc ccggtggacg ccgccacacc gcggctgaag
     1801 ctggtcggcg cgcgccagca caacctgcgc gacctcacgg tcggtttccc gctgcggcgc
     1861 ctgacctgcc tgaccggcgt gtccggctcg ggcaaatcga cgctgatcca ggacgtgctg
     1921 ttcccggcgc tggccaagca tttcggggaa gcggcggaat cgccgggggt cttcgatcgt
     1981 ctcgagaatg tggataactt gcgcggcgtg gtcatggtgg accagtcgcc gatcggcaag
     2041 agctcgcgct ccaacccggt gagctacgtc ggcgcctggg acccgatccg tgccctcttc
     2101 gccgccctgc cggaggcgaa gcagcgcggc tatacccccg gcaccttcag cttcaacgcc
     2161 ggccacggcc gctgcccgac ctgcaccggc tcgggcttcg agcacgtcga gatgcaattc
     2221 ctgtccgacg tgtggctgcg ctgcccggac tgcgacggca agcgttatcg gccggaagtg
     2281 cttgaattgc aatggaacgg gctgtcggtg gccgacgtgc tggagctcac cgtgcatgag
     2341 gcgctggatg tgttccgcga gcatcccaag gtgctcgccg cgctcgcgcc gctcgccgac
     2401 gtcggcctgg attacctgcg cctcggccag cccgtgccga cgctgtcggg tggcgaggcg
     2461 cagcgcctca agctcgccgg ccacctcgcc gaggccgcgc agaagaagcc tgtacgcaaa
     2521 gccagtattg tggataagtt tggggacaag gctggggaca agaccgagcc cggcctgctc
     2581 ttcctcttcg acgagcccac caccggcctg cacttcgagg acgtcgccaa gctgctcggc
     2641 gccttcgaca agctgctgca ggcgggccat tcgctgatcg tgatcgagca caacctcgac
     2701 gtgatcgctg cttccgactg gatcatcgac ctcggccccg agggcggcga aggcggcggc
     2761 gcgatcgtgg tcgaaggccc gccggaggcg gtgcgcacgc acgcgagctc gcacaccggt
     2821 gccgcgctgc gcgactacgc cgaggcgctt cgaaaggtcg gggatggcgg ccggagctcc
     2881 gccgacgcgg aggctccggg ttcccgtagg agcggcgcaa gccgcgatca cggcgcttcc
     2941 ggcagcgccg tcgccacgga aagcccaatc gcggcttgcg ccgctcctac gggggatcgg
     3001 gcgctcgtcg ccgcggagcc gcgggtggaa taccgccccg tagtcgcccc cgcgatcgaa
     3061 atccgccacg cccgcgagca caacctcaag aacgtcagcc tgcagatccc gcgcgaccag
     3121 ttcaccgtga tcaccggcct gtccggctcg ggcaagtcca cgctggcctt cgacatcgtc
     3181 ttcgg
//
LOCUS       DAOQNN010000381         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_21777_length_3146_cov_11.187318, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000381 DAOQNN010000000
VERSION     DAOQNN010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21777_length_3146_cov_11.187318"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PKV97_14885"
     CDS             complement(<1..1181)
                     /locus_tag="PKV97_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HNB07216.1"
                     /translation="MTPVEQPVSAAAAAADAAADRDCYHCGLPIPPETRHYVRIDGRE
                     RRMCCVGCEAVAQSIVDNGLVDYYRHRDAMPETRREAMPVELQELGLFDHPDFQKSFV
                     RPVGEHEREAALILEGITCAACVWLNENHVARLSGVSAIEINYATRRARVRWDERRIK
                     LSDILAAIQAIGYRAYPYDAERSEQLAHRERRSMLWRVFVAGFGMMQVMMYAFPVYVA
                     GEGDMTWDIEQLLRWASLLLTLPVVLYSAAPFFQRAWRDLRLRRLGMDVPVALGVGSA
                     FAASLWATLTDGPEVYFDSVTMFVFFLLGGRYLEMIARQKAVRGVEELGKALPSFAER
                     IAGWPGESAGERVPTSQLVAGDVLRVRPGEVIPVDGLVVEGRGEANEALLTGESMPVP
                     KA"
     gene            complement(1319..2086)
                     /locus_tag="PKV97_14890"
     CDS             complement(1319..2086)
                     /locus_tag="PKV97_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB07217.1"
                     /translation="MQIENGVFVITGGGSGLGAASARMLVEAGGRVVLADVNAQAGEA
                     LAAELGAAACFVQTDVTDADSAKAAIDTAVQRFGGLNGLVSCAGVAPAEKVVGREGPH
                     RLESFARTVSINLIGTFNMLRLAADVMSKAEPNAGGERGVIVNTASVAAFDGQVGQAG
                     YASSKAGVVGLTLPVARELSRFGIRVMAIAPGIMETPMLMGMPQEVQDSLGKMVPFPS
                     RLGKPAEFAALVRHIVENAYLNGEVIRLDGAIRMAAK"
     gene            complement(2291..2824)
                     /locus_tag="PKV97_14895"
     CDS             complement(2291..2824)
                     /locus_tag="PKV97_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNB07218.1"
                     /translation="MKTSLILTLVGPDRPGLVSAVSACAGAHGANWMESRLTRLAGQF
                     AGVVRLEVDAAAADALERGLNGLVAEGLKLAVVRADAGAAAQARLARLELVGHDRPGI
                     VRDISAVLARHGLSIDALETACESASMSGEPLFRARAELAVPAGIGLAAVRNDLEALA
                     NELMVDLDFEEVSEPAG"
BASE COUNT          542 a         1138 c         1039 g          427 t
ORIGIN      
        1 gccttgggga ccggcatgct ctcgccggtc agcagcgcct cgttggcctc gccgcgaccc
       61 tcgaccacca gaccatcgac cgggatcacc tcgcccgggc gcacacgcag cacgtccccc
      121 gccaccagct gcgaggtcgg cacgcgttcg ccggcggact cccccggcca gccggcgatg
      181 cgctcggcga acgagggcaa ggctttgccc aattcctcca ccccgcgcac cgccttctgg
      241 cgcgcgatca tctcgaggta gcgcccgccg agcaggaaga acacgaacat cgtcaccgaa
      301 tcgaaataga cttccggccc gtcggtcagc gtcgcccaca ggctggcggc gaaggcgctg
      361 cccacgccga gcgcgaccgg cacgtccatg cccaggcggc gcaggcgcag gtcgcgccag
      421 gcgcgctgga agaagggcgc ggccgaatac agcaccaccg ggagcgtcag cagcaggctg
      481 gcccagcgca gcagctgctc gatgtcccag gtcatgtcgc cctcgccggc cacgtagacc
      541 gggaaggcgt acatcatcac ctgcatcatg ccgaagccgg cgacgaacac ccgccacagc
      601 atcgagcgcc gctcacgatg ggcgagctgc tcggaacgct cggcgtcgta gggataggcg
      661 cggtagccga tcgcctggat cgcagcgagg atgtcggaaa gcttgatccg gcgctcgtcc
      721 cagcgcacgc gcgcacgccg ggtggcgtag ttgatctcga tcgccgacac gccggacagg
      781 cgggcgacgt ggttctcgtt gagccacacg caggcggcgc aggtgatgcc ctcgaggatc
      841 aacgcggctt cgcgctcgtg ctcgccgacc gggcgcacga agctcttctg gaagtcgggg
      901 tgatcgaaga ggccgagctc ctgcagttcg accggcatgg cctcgcgccg cgtctcgggc
      961 atcgcgtcgc gatggcggta gtaatccacc aggccgttat cgacgatcga ctgcgccacc
     1021 gcctcgcagc cgacgcagca catgcgccgc tcgcggccgt cgatgcgcac gtagtgccgc
     1081 gtctcgggag ggatcggcag accgcagtga tagcagtcgc gatccgcggc ggcatcggct
     1141 gcggcggcgg ccgcggaaac gggctgttct acaggagtca tcggcgccaa gcgaaaccgc
     1201 cccgagcgca catgcgcggg gcgggaagaa ttggaaccga tttaataaca taattcaccc
     1261 gccccggcta ttgcaccgca gcatcgccgg caggcggctg ctgcggtgcg acgccttctt
     1321 acttggcggc catgcggatc gcgccgtcga ggcggatcac ctcgccgttc aggtaggcgt
     1381 tctcgacgat gtggcgcacc agcgcggcga actcggcggg cttgcccaga cgcgagggga
     1441 agggaaccat cttgccgagc gagtcctgca cctcttgcgg catgcccatc agcatcggcg
     1501 tctccatgat gccgggggcg atggccatca cgcggatgcc gaagcgggac agctcgcgcg
     1561 ccaccggcag cgtgagtccg accacgccag ccttggacga ggcgtagccg gcctgtccga
     1621 cctggccgtc gaaggcggcc accgacgcgg tattcacgat cacgccacgc tcgccgccgg
     1681 cattgggctc ggccttgctc atgacgtcgg cggcgaggcg caacatgttg aaggtgccga
     1741 tcaggttgat cgaaacggta cgtgcgaagc tctcgagccg atgcggcccc tcgcggccca
     1801 ccaccttttc ggctggcgcc acaccggcgc agctgaccag gccattgagg cctccgaagc
     1861 gctgcaccgc agtatcgatc gccgccttgg cgctgtcggc atcggtgacg tcggtctgca
     1921 cgaagcaggc cgcagcgccg agctcggcgg cgagcgcctc gcccgcctgc gcgttgacgt
     1981 cggccagcac cacgcggcca ccggcttcga ccagcatgcg cgcgctcgcc gcgcccaggc
     2041 ccgagccacc acccgtgatc acgaatacgc cattctcgat ctgcatcgcc tttctcctcg
     2101 aaaaatcggt gacggcggac gccgtccgct gcagcgcagc accttaaatt acgttgacgt
     2161 aggcgtcaat cggatcgcca cgtcgctgcc ccaacgtggc acgcgggcaa gcgcacgcga
     2221 acaccgcgga gccccggcat gtcgagcgga tgcgcggcac gcgccacgcc cccgcgaact
     2281 ccgcgagtcc tcagccggcc ggctcggaca cttcctcgaa atcgagatcg accatcagct
     2341 cgttcgcgag cgcctccagg tcgttgcgca ccgcagcgag gccgatccct gccggcaccg
     2401 ccagctccgc gcgcgcgcga aagagcggct cgccgctcat cgaggcactc tcgcaagccg
     2461 tctccagcgc atcgatgctg aggccgtggc gagcgagcac ggccgaaatg tcgcgcacga
     2521 tgccgggacg atcgtggccg acgagctcga gacgcgccag gcgcgcctgc gcagcggcgc
     2581 ccgcatcggc ccgcacgacg gccagcttga gcccttccgc aaccagcccg ttcaggccgc
     2641 gctcgagcgc gtcggccgcc gcggcatcca cttcgaggcg cacgacgccg gcgaactgcc
     2701 cggccagccg ggtcaggcgg ctctccatcc agttcgcgcc gtgggcgccg gcgcaggccg
     2761 acaccgcact caccaggccg ggacgatcgg ggccgaccag ggtaaggatc agggaagtct
     2821 tcatgttcgg tctccgtccg gccccatcag cggcgctccg cgtccctcgg cgcggaatcg
     2881 agggcgatcg ctgcaaggcc ggagacaatt ggaatgggaa ccgacacgat aacccagcgg
     2941 cctcgcaagc gggagcccgg cggcccgggc aggacgcacg ccctcggcga ctcggcacat
     3001 cgcttccgcg aggcgcttgc aggacggcta catgccagct gaaaagcaaa aagggcgacc
     3061 ccgaaggacc gccctgagtg cttgaaacca ttggtgccgg agaagagact cgaactcccg
     3121 accttcgcat tacgaatgcg ctgctc
//
LOCUS       DAOQNN010000382         3131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_21937_length_3131_cov_10.300715, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000382 DAOQNN010000000
VERSION     DAOQNN010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21937_length_3131_cov_10.300715"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PKV97_14900"
     CDS             <1..844
                     /locus_tag="PKV97_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNB07219.1"
                     /translation="KGVTEETTTGVHRLYQMHARGELRFPAINVNDSVTKSKFDNLYG
                     CRESLVDGIKRATDVMIAGKVAVVAGYGDVGKGSAQALRALSAQVWVTEVDPICALQA
                     AMEGYRVVTMEYAADKADIFVTATGNYHVITHDHMAKMKDQAIVCNIGHFDNEIDVAS
                     IEQYQWEEIKPQVDHVIFPDGKRIILLAKGRLVNLGCATGHPSYVMSSSFANQTIAQI
                     ELFTRTADYPIGVYTLPKHLDEKVARLQLKKLNVQLTELTDAQAAYIGVPKQGPYKSD
                     AYRY"
     gene            935..1792
                     /locus_tag="PKV97_14905"
     CDS             935..1792
                     /locus_tag="PKV97_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNB07220.1"
                     /translation="MSMNSFHARQGKPRPAAQGKGPADRHGLLRVDQLLVAQGLAASR
                     TAARALIEAGRVSHEGQPVAKPALELPPDARLAVTPADSDRFVSRGALKLEGALARSG
                     LDVRGATCLDIGQSTGGFTDCLLQAGAARVVGVEVGHNQLHPRLRADGRCVTLEGVNA
                     RQLDAAALGGHYPPHGFDLLVCDASFISLALLLPQWAALLAPAGHVLALVKPQFEVGP
                     QGLGKGGIVRDPARYTEVEVRLRAAADTAGLVILDWFDSPITGGDGNREFFFHAIRRP
                     GGDTHRSEP"
     gene            1789..2622
                     /gene="metF"
                     /locus_tag="PKV97_14910"
     CDS             1789..2622
                     /gene="metF"
                     /locus_tag="PKV97_14910"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNB07221.1"
                     /translation="MKPEISIEFFPPTTAEGAEKLHATREKLAALEPAFFSVTYGAGG
                     STRERTFATVKEIAAAGHEAAPHLSCVGSSRDSIREILQEYAAAGIRRIVALRGDLPS
                     GAGAAGEFRYASELVEFIRSETGDRFRIEVAAYPEWHPQAKSPKDDLAAFKRKVEAGA
                     DSAITQYFYNLDAYLNFVDAVRRMGVDVPIVPGIMPIGSFSKLARFSDACGAEIPRWM
                     RKTFESYGDDSDAIRAFGLDVVTRLCEKLLAAGAPGLHFYSMNMAGPTTEICRRLGLT
                     A"
     gene            2763..>3131
                     /locus_tag="PKV97_14915"
     CDS             2763..>3131
                     /locus_tag="PKV97_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB07222.1"
                     /translation="MHFAPFSSGPAQGIDRCVAGADLCISARRQLARLLLTGALALPF
                     AAAAEAPPDLSLEQLIALPVESAAEYERVAVVGASKYEQTKDRVAAAVTAITRDEIRS
                     FGWRTLDEALATLPGVHTTYD"
BASE COUNT          506 a         1170 c         1010 g          445 t
ORIGIN      
        1 caagggcgtg accgaggaga ccaccaccgg cgtgcatcgc ctgtaccaga tgcacgcgcg
       61 cggcgagctc aggttcccgg cgatcaacgt caacgattcg gtcaccaagt cgaagttcga
      121 caacctctac ggctgccgcg aatcgctggt cgacggcatc aagcgcgcca ccgacgtgat
      181 gatcgccggc aaggtcgcgg tggtggccgg ctacggtgac gtgggcaagg gctcggccca
      241 ggccctgcgc gcgctgtcgg cccaggtgtg ggtgaccgag gtcgacccga tctgcgcgct
      301 gcaggcggcg atggaaggct accgcgtggt cacgatggaa tacgccgccg acaaggccga
      361 catcttcgtc accgcgaccg gcaactacca cgtgatcacc cacgaccaca tggcgaagat
      421 gaaggaccag gccatcgtct gcaacatcgg ccacttcgac aacgagatcg acgtcgccag
      481 catcgagcag taccagtggg aggagatcaa gccgcaggtc gatcacgtga tcttccccga
      541 cggcaagagg atcatcctgc tcgccaaggg ccgcctggtg aacctcggct gcgccaccgg
      601 ccacccgagc tacgtgatgt cgagctcctt cgccaaccag acgatcgcac agatcgagct
      661 gttcacccgc acggccgact acccgatcgg cgtgtacacc ctgcccaagc acctcgacga
      721 gaaggtcgcc cgcctgcagc tgaagaagct caacgtgcaa ctcaccgagc tgaccgacgc
      781 gcaggccgcc tacatcggcg tgcccaagca aggcccgtac aagtcggacg cgtaccgcta
      841 ctgagcccgg gggcggcgca gcccccggca ggagcgcctt ccggcgctcc tgtacaaagg
      901 ctcttcacgc ctcaccccct gactcctcac gcccatgagc atgaactcct tccacgcccg
      961 ccagggcaag ccgcggccgg ccgcgcaggg caagggcccg gcggaccgcc acggcctgct
     1021 gcgcgtcgac cagctgctgg tggcgcaggg gctggcggcc tcgcgcaccg cggcgcgcgc
     1081 gctgatcgag gccgggcggg tgagccacga gggccagccg gtcgccaagc cggcgctgga
     1141 attgccgccc gacgcacggc tcgccgtcac ccccgccgac agcgaccgct tcgtctcgcg
     1201 cggcgcgctc aagctcgaag gcgcgctcgc gcgcagcggg ctggacgtac gcggcgccac
     1261 ctgcctcgac atcggccaat ccaccggcgg cttcaccgac tgcctgttgc aggccggcgc
     1321 agcgagggtg gtcggggtgg aggtcggcca caaccagctc catccgcgcc tgcgcgccga
     1381 tgggcgctgc gtcaccctgg agggcgtcaa cgcccgccag ctcgacgccg ccgcgctcgg
     1441 cggacactac ccgccgcacg gcttcgacct gctggtgtgc gacgccagct tcatctcgct
     1501 cgccctgctg ctgccgcagt gggccgcgct gctcgcaccc gccggccacg tgctggcgct
     1561 ggtcaagccg cagttcgagg tcggcccgca aggcctgggc aagggcggca tcgtgcgcga
     1621 ccccgcgcgc tacaccgagg tcgaggtccg cctgcgcgcc gcagcggaca ccgccggcct
     1681 cgtcatcctc gactggttcg actcgccgat caccggcggc gacggcaacc gcgaattctt
     1741 cttccacgcc atccgcaggc cgggcggcga cacccaccga agcgaaccat gaaacccgaa
     1801 atctccatcg agttcttccc gcccaccacg gccgagggcg ccgagaagct gcatgccacc
     1861 cgggagaagc tcgccgcgct cgagccggcc ttcttctcgg tgacctacgg cgccggcggc
     1921 tccacccgcg agcgcacctt cgccaccgtg aaggagatcg ccgccgccgg ccacgaggcc
     1981 gcgccacacc tgtcctgcgt gggctcgtcg cgcgacagca tccgcgagat cctgcaggaa
     2041 tacgccgccg ccggcatccg ccgcatcgtc gcgctgcgcg gcgatctgcc ctcgggcgcc
     2101 ggcgcggcgg gcgagttccg ctacgccagc gagctcgtcg agttcatccg cagcgagacg
     2161 ggcgaccgct tccgcatcga ggtcgccgcc taccccgagt ggcacccgca ggcgaagagc
     2221 ccgaaggacg acctcgccgc cttcaagcgc aaggtcgagg ccggcgccga ctcggcgatc
     2281 acgcagtatt tctacaacct cgacgcctac ctgaacttcg tcgacgcggt gcgcaggatg
     2341 ggcgtcgacg tgccgatcgt gcccggcatc atgccgatcg gcagcttcag caagctggcg
     2401 cgcttctcgg atgcctgcgg cgccgagatt ccgcgctgga tgcgcaagac cttcgagagc
     2461 tacggcgacg acagcgacgc gatccgcgcc ttcggcctcg acgtggtcac ccggctgtgc
     2521 gagaagctgc tcgccgcggg cgcgcccggc ctgcatttct acagcatgaa catggccggc
     2581 ccgaccaccg agatctgccg gcggctgggg ctgactgcct gatctcccgg caatcctgga
     2641 cgggatcgta aggtgggtgt gacgtatgcg gctcaagccc ctgtggcgta tgccgtatag
     2701 tccttcgatc agcacatctt cttccgcccc acgcctcgtg ctggcggcca ggatccgcca
     2761 ccatgcattt cgcgcctttc tcctccggtc ctgcccaagg cattgatcgg tgcgtggctg
     2821 gcgcggacct ctgcatatct gcccggcgcc aactggcccg gctgctgttg accggcgccc
     2881 tcgcgctgcc cttcgccgcg gcggccgagg cgccccctga tctgagcctg gagcagttga
     2941 ttgcgcttcc ggtggagagc gcggcagagt acgagcgggt cgcggtggtc ggcgcctcaa
     3001 aatacgagca gacgaaggac cgcgttgccg ccgccgtcac cgcgatcacc cgcgacgaga
     3061 tccgcagctt cggctggcgc acgctcgacg aggcgctcgc caccctcccc ggcgtgcata
     3121 ccacctacga c
//
LOCUS       DAOQNN010000383         3129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_21960_length_3129_cov_7.309369, whole genome shotgun sequence.
ACCESSION   DAOQNN010000383 DAOQNN010000000
VERSION     DAOQNN010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21960_length_3129_cov_7.309369"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="PKV97_14920"
     CDS             complement(<1..1299)
                     /locus_tag="PKV97_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB07223.1"
                     /translation="MKKPLLLLLVLALVAGFFASGLHRQLDLDTLKAGMAGFAAWREA
                     SPVLVAALYFAAYVAVTALSLPGAAVMTLAGGALFGLGWGLLIVSFASTIGATLAFLV
                     SRHLLRDSVHARFGARLRAIDEGIARDGAFYLFSLRLVPAFPFFLINLLMGLTPIRTR
                     TFYWVSQLGMLPGTLVYVNAGTELGAVDSLAGVLSPGLVASFVLLGLFPLLARWMVER
                     VQARRVYAGWQRPARFERNLVVIGAGAAGLVTSYIAAAVKAKVTLVEAGRMGGDCLNT
                     GCVPSKALIRSARLAHQIRHADRYGLEPGEPEIDFRRLMARVRAVIRKIEPHDSVERY
                     SALGVEVLQGRARIVDPWTVEVALNEGGTRRLSTRSIVIATGARPTVPDLPGLEAVGY
                     LTSDTVWDAFAALDAPPRRLLVLGGGPIGCELTQAFARFGA"
     gene            complement(1346..2455)
                     /locus_tag="PKV97_14925"
     CDS             complement(1346..2455)
                     /locus_tag="PKV97_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB07224.1"
                     /translation="MHELVQDYYGRTLQSSADLRTSACCDASAPPPALRPVLGRIHPE
                     VLARYYGCGLVSPPLLEGCRVLDLGCGSGRDVYALAQLVGEHGEVVGVDMTAEQLAVA
                     RRHEDYHREAFGFARSNVRFLEGYIERLGELGLEDASFDVIVSNCVINLCPDKAAVLR
                     EVFRLLRRGGEFYFSDVYADRRVPAAVRDDPVLYGECLGGALYWNDFLALARAAGFAD
                     PRLVEDRPLAATDPALAAKLGDVRFFSATWRLFRIDGLESACEDFGQAVVYRGTLPEQ
                     ATRFVLDKRHGFDAGRVVPVSGNTWRMLHDSRLRPHFDFIGSFERHYGRFTAEGPAMP
                     FDTAARGTVTDNPAPTTMPAPVPATLGSAARKSCC"
     gene            complement(2525..>3129)
                     /locus_tag="PKV97_14930"
     CDS             complement(2525..>3129)
                     /locus_tag="PKV97_14930"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB07225.1"
                     /translation="GMAGGLLAGHAEGARWRVLRRLAAAGIALHPARARGTPAGLELV
                     DAGRDAPTTLPADCVIAATGAHAPPWTQSSGLACDAAGWVMVDAGHRSVSHPFIHAAG
                     DVCTRADPGFARSGVHAVHAGPVLAANLLAALAGGPASAPVRSYRPRPRSLYLIASGP
                     RHAIASWGRWSAEGRWVWRWKDRIDRGFIARHRRAAIVPV"
BASE COUNT          473 a         1101 c         1175 g          380 t
ORIGIN      
        1 cgctccgaag cgggcgaagg cctgcgtcag ctcgcagccg atcggcccgc cgccgagcac
       61 cagcagccgg cgcggcggcg cgtcgagcgc ggcgaaggcg tcccataccg tgtcgctggt
      121 gaggtagccc accgcctcca gcccgggcag gtcgggcacg gtcgggcggg cgccggtggc
      181 gatcacgatg ctgcgggtgc tgagccggcg cgtgccgccc tcgttcagcg ccacctccac
      241 cgtccatggg tcgacgatgc gcgcgcgccc ctgcagcacc tcgacgccga gcgcgctgta
      301 gcgctccacg ctgtcgtgcg gctcgatctt gcggattacc gcgcgcacgc gcgccatcag
      361 cctgcggaag tcgatctccg gctcgccggg ctccaggccg tagcggtcgg cgtggcggat
      421 ctggtgggcc agcctggcgc tgcggatcag cgccttgctc ggcacgcagc cggtgttgag
      481 gcagtcgccg cccatgcgtc cggcctcgac cagggtcacc ttcgccttca ccgccgccgc
      541 gatgtaggac gtcaccagcc cggcggcgcc ggcgccgatc accaccaggt tgcgctcgaa
      601 gcgtgccggg cgctgccacc ccgcatacac ccggcgcgcc tgcacgcgct cgaccatcca
      661 gcgtgcgagc agcgggaaga ggccgagcag cacgaaggag gcgacgaggc ccggagacag
      721 cacgccggcc aggctgtcca ccgcgccgag ctcggtgccc gcgttcacat acaccagcgt
      781 gccgggcagc atgccgagct ggctcaccca gtagaaggtg cgcgtgcgga tcggggtgag
      841 ccccatcagc aggttgatca ggaagaaggg gaaggccggc accaggcgca gcgagaacag
      901 gtagaacgcg ccgtcgcgcg cgatgccctc gtcgatcgcg cgcaggcgcg cgccgaagcg
      961 cgcgtgcacg ctgtcgcgca gcaggtggcg cgagaccagg aaggcgagcg tggcgccgat
     1021 ggtcgacgcg aaggacacga tcagcagccc ccagcccagc ccgaacagtg ccccgccggc
     1081 cagggtcatc accgccgcgc cgggcagcga tagcgcggtc accgccacgt aggcggcgaa
     1141 atagagggcg gcgaccagga ccggcgaggc ctcgcgccag gcagcgaacc cggccatgcc
     1201 ggccttgagg gtgtcgaggt cgagctggcg gtgcagcccg ctggcgaaga agccggcgac
     1261 gagcgcgagc acgagcagca gcagaagggg tttcttcatc ttcgtcccgg gagcgggtga
     1321 gccgtgcgga gccgtcgtgg cccgatcagc agcaggattt gcgcgccgcg ctgccgaggg
     1381 tcgcggggac cggcgccggc attgtcgtcg gcgccgggtt gtccgtgacg gtgccgcgcg
     1441 ccgccgtgtc gaagggcatc gccgggccct ctgcggtgaa gcggccgtaa tggcgctcga
     1501 agctgccgat gaagtcgaag tgcggccgca gccggctgtc atgcagcatg cgccaggtgt
     1561 tgccgctcac cggtacgacc cggccggcgt cgaagccgtg ccgcttgtcg agcacgaagc
     1621 gcgtggcttg ctcgggcagc gtgccgcgat acaccaccgc ctggccgaag tcctcgcagg
     1681 cgctctccag tccgtctatc cggaacagcc gccaggtcgc cgagaagaag cggacgtcgc
     1741 ccagcttcgc ggccagcgcg gggtcggtcg ccgcgagcgg ccggtcctcc acgaggcgcg
     1801 ggtcggcgaa gcctgcggcg cgggcgagcg cgaggaagtc gttccagtac agcgcgccgc
     1861 ccaggcattc gccgtacagc accggatcgt cgcgcacggc ggcgggcacg cggcggtcgg
     1921 catacacgtc cgagaagtag aactcgccac cccggcgaag caggcggaag acctcgcgca
     1981 gcaccgcggc cttgtccggg cacaggttga tcacgcagtt cgacacgatc acgtcgaagc
     2041 tggcgtcctc caggccgagc tcgcccaggc gctcgatata gccctcgagg aagcgcacgt
     2101 tgctgcgcgc gaagccgaag gcctcgcggt ggtagtcctc gtggcggcgc gcgacggcga
     2161 gctgctccgc ggtcatgtcc acgccgacga cctcgccatg ctcgccaacg agctgggcga
     2221 gcgcatacac gtcgcgcccc gaaccgcagc cgaggtcgag cacccggcag ccttcgagca
     2281 ggggcgggct caccaggccg cagccgtagt agcgcgcgag cacttcgggg tggatgcgcc
     2341 cgagcaccgg cctgagcgcc ggcggcgggg cgctggcatc gcagcaggcc gaggtcctga
     2401 ggtcggcgga ggattgcagg gtgcggccgt agtagtcctg gacgagttcg tgcatggtgg
     2461 ggccccgggg aataagggag tgagaaggga gcttcgggct ttgtcgcatg tgctccgcgg
     2521 attcttacac cggcacgatg gctgcgcgcc ggtgacgtgc aatgaatcct cggtcgatgc
     2581 ggtccttcca gcgccacacc cagcgcccct cggcggacca gcgtccccac gaggcgatgg
     2641 cgtggcgcgg accgcttgcg atcaggtaga gcgaacgcgg gcgcggccgg tagctgcgca
     2701 ccggcgcgct cgccgggccg cccgccagtg cggcgagcag gttggcggcg agcaccgggc
     2761 cggcatggac cgcgtgcacg cccgagcgcg cgaagccggg atccgcgcgg gtgcacacgt
     2821 cgccggcggc gtggatgaaa ggatgcgaga cgctgcgatg gccggcgtcg accatgaccc
     2881 agcccgcggc gtcgcaggcc aggccgctgc tttgcgtcca gggcggggcg tgtgcgccgg
     2941 tggcggcgat cacgcagtcc gcgggcagcg ttgtcggcgc gtcacgcccg gcgtccacaa
     3001 gctcgagccc cgcaggcgtg ccccgtgcac gagccgggtg gagcgcgatg ccggccgcgg
     3061 ccagacggcg cagcacgcgc caccgcgcac cttcggcatg gccggcgagc agcccgccgg
     3121 ccatgccga
//
LOCUS       DAOQNN010000384         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_22169_length_3110_cov_8.774468, whole genome shotgun sequence.
ACCESSION   DAOQNN010000384 DAOQNN010000000
VERSION     DAOQNN010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22169_length_3110_cov_8.774468"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PKV97_14935"
     CDS             complement(<1..380)
                     /locus_tag="PKV97_14935"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924544.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNB07226.1"
                     /translation="MRASQFHLFTLKEAPSDAEVVSQKLMLRAGMIRKVAAGIYSYMP
                     MGLRAIRKVEAIVREEMDRAGAMELIMPMVQPAELWDETGRWDKMGDELLRFKDRHER
                     DFALQPTSEEVVTDIARQELKSYR"
     gene            548..1141
                     /locus_tag="PKV97_14940"
     CDS             548..1141
                     /locus_tag="PKV97_14940"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNB07227.1"
                     /translation="MLDREGFRPNVGIILVNARNEVFWGKRIREHSWQFPQGGIKHGE
                     SPEQAMYRELHEEVGLRPEHVRILGRTRGWLRYEVPKHWIRREWRNTYRGQKQIWFLL
                     RLVGRDSDVCLRASTHPEFDAWRWSNYWVPLEAVIEFKRQVYQLALIELARILFRSRS
                     VELPDSYRLPEPEPAPEPVRVVLGERAEHGLRDAMRK"
     gene            1319..1852
                     /locus_tag="PKV97_14945"
     CDS             1319..1852
                     /locus_tag="PKV97_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HNB07228.1"
                     /translation="MRPSLGALPRAGVVALALLSVPASAGFLSDADPDWKEGEATMPA
                     APREAALRAIDVGTPSPNAFFVDEDSVGVGADGVVRYTLVVRTRGGAENVSFEGIRCA
                     TFERRIYASGRKDGTWAALKNSAWQPIVESGYNRPRAALAHDYFCDGPAGPRDREHAL
                     RLLRERRDPIDPSGVHQ"
     gene            1849..2469
                     /gene="coq7"
                     /locus_tag="PKV97_14950"
     CDS             1849..2469
                     /gene="coq7"
                     /locus_tag="PKV97_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HNB07229.1"
                     /translation="MIDQAIIEFDKALRTVFASARSVRAMPGEGVPDAELGAAERSHA
                     AALMRVNHVGEVCAQALYQGQSIMSREPAIRDTLRQAAQEETEHLAWTERRIAELGGR
                     KSLLNPLWYGGALALGLAAGRFGERWNLGFLAETERQVEQHLKSHLDTLPAADRRSRA
                     IVEQMKVDEAEHAATALRLGAHELPAPAKGAMKLAARVMTTLAYRL"
     gene            complement(2525..2944)
                     /locus_tag="PKV97_14955"
     CDS             complement(2525..2944)
                     /locus_tag="PKV97_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB07230.1"
                     /translation="MECTVKWLDGMSFIAETGTGHLVAMDGAPDAGGRNLAPRPMELL
                     LAGAGGCTAFDVMLILKRGRHDVRGCSVRLEAERAESDPKVFTRIRFIYTITGRKLKR
                     ETVERAVHLSAEKYCSASIMLGKTAEMIHEVELVEAD"
BASE COUNT          443 a         1033 c         1096 g          538 t
ORIGIN      
        1 tggcggtagc tcttgagctc ctggcgcgcg atgtcggtga cgacctcctc ggaggtgggt
       61 tgcagcgcga agtcgcgctc gtggcggtcc ttgaagcgca gcagctcgtc gcccatcttg
      121 tcccagcgcc cggtctcgtc ccacagctcg gcgggctgca ccatcggcat gatcagttcc
      181 atggcgccgg cgcggtccat ctcctcgcgc acgatggcct cgaccttgcg gatcgcgcgc
      241 aggcccatcg gcatgtagct gtagatgccg gcggcgacct tgcggatcat gccggcgcgc
      301 agcatcagct tctggctgac gacctcggcg tcggaggggg cttccttgag ggtgaagaga
      361 tggaactggc tggcgcgcat gggagtcttc ggatcaggcg tggacaagcg cgcgattcta
      421 gcgcatgggg ccggcgcgca agcgttcctc gacctccagc ttagtggatg caccgtggca
      481 gtgcgctttc attgcgtcgc caagtgtgaa aaaatcgtga ccaatcaaac gaattcgaag
      541 gttcgtcatg cttgaccgtg aaggctttcg cccgaacgtc ggcatcattc tggtcaatgc
      601 gcgcaacgag gtcttctggg gcaagcgcat ccgcgaacat tcctggcagt ttccacaagg
      661 tggcatcaag cacggcgagt cgccggagca ggccatgtat cgggaactcc acgaagaggt
      721 cggcctgcgg cccgagcacg tcaggatcct cggccgtacg cgcggatggt tgcgctatga
      781 ggttcccaag cactggatcc ggcgcgagtg gcgcaacacc taccgtgggc agaagcagat
      841 ctggttcctg ctccgcctgg tggggcgcga ctccgacgtc tgtctgcgcg cgagcactca
      901 tccggaattc gatgcctggc gctggagcaa ctactgggtg ccgctggaag cggtgatcga
      961 attcaagcgc caggtgtatc agctggcctt gatcgaactg gcgcgcatcc tgtttcgttc
     1021 gcgcagcgtc gagttgccgg acagctatcg actgcccgaa ccggaacccg ctcccgagcc
     1081 ggtgcgggtg gtgctcggcg agcgcgccga gcacggactg cgcgatgcga tgcggaagta
     1141 gcccgactcc gtcctgccgc acggcgcagg ccggcggcgg gctggctccg tgcggccggc
     1201 gcttgcccgc cgcggcggga ccgtgtgccg tgggcgcgcc ggtcctgctt cggcgctgcc
     1261 cgcttccttc ctccccgatg cggagttcct actcttgagt cctttcccga gccgtttctt
     1321 gcgcccctcg ctcggtgcac ttccacgcgc cggcgttgtc gccctcgccc tcctgtcggt
     1381 gcccgcgtcc gcggggtttc tgtcggatgc cgatcccgac tggaaggagg gcgaggcgac
     1441 gatgccggcc gcgccgcgcg aggccgcgct gcgtgcgatc gacgtcggca cgccttctcc
     1501 caatgccttc ttcgtcgacg aggacagcgt cggcgtcggg gcggatggcg tggtgcgcta
     1561 caccttggtc gtacgcacgc gcggcggcgc ggagaacgtc agcttcgagg gcatccgctg
     1621 cgccaccttc gagcgccgga tctacgccag cggacgcaag gacgggacct gggcggcgct
     1681 gaaaaacagt gcctggcagc ccatcgtcga aagcggctac aaccgcccgc gcgcggcgct
     1741 ggcgcacgac tatttctgcg acggcccggc cgggccgcgc gaccgcgagc atgcgttgcg
     1801 cctgctgcgg gagcggcgcg accccatcga cccgtctgga gtgcatcaat gatcgatcag
     1861 gccatcatcg agttcgacaa ggccctgcgg acggtcttcg cgtcggcgcg cagcgtgcgg
     1921 gcgatgccgg gcgagggcgt gcccgatgcc gagctgggcg cagccgagcg cagccatgcg
     1981 gcggcgctga tgcgggtgaa tcatgtcggc gaggtgtgcg cgcaggcgct ctaccagggg
     2041 cagtccatca tgtcgcgcga gcccgcgatt cgcgacaccc tgcgccaggc cgcgcaggag
     2101 gagaccgagc acctggcgtg gaccgagcgc cgcatcgccg agctcggtgg tcgcaagagc
     2161 ctgctcaatc cgctgtggta tggcggcgcg ctcgccctcg ggctggcggc ggggcgcttc
     2221 ggcgagcgct ggaacctcgg cttcctggcc gagaccgagc gccaggtcga gcagcacctc
     2281 aagagccatc tcgacaccct gccggccgcg gatcggcgct cgcgcgcgat cgtcgagcag
     2341 atgaaggtgg acgaggccga gcacgccgcg acggcgctgc gcctgggtgc gcacgagttg
     2401 ccggcaccgg cgaagggggc gatgaagctc gccgcgcggg tgatgacgac gctcgcctac
     2461 cggctctgag cgattcccgt gccctcgtcc gcgtcgcgag ggcagcctcg cgccgccgcg
     2521 gtcctcagtc cgcctcgacc agctcgacct cgtggatcat ctcggcggtc ttgccgagca
     2581 tgatcgaggc cgagcagtat ttttccgccg acaggtgcac cgcgcgctcg accgtctcgc
     2641 gcttgagctt gcgaccggtg atcgtgtaga tgaagcggat gcgggtgaac accttggggt
     2701 cggactccgc gcgctcggct tcgaggcgca cgctgcagcc gcgcacatcg tggcgtccgc
     2761 gcttgaggat cagcatgacg tcgaaggcgg tgcagccacc ggcgccggcc agcagcaact
     2821 ccatcggccg tggagccagg ttgcgcccgc cggcatcggg cgcgccgtcc atcgcgacga
     2881 ggtggccggt gccggtttcg gcgatgaagc tcatgccgtc gagccacttg accgtgcatt
     2941 ccatgctgtt cgctccttcg ggttctggtc ccgttgtcgg gttcgttggg ggttggggcg
     3001 gcatgttacc gcagtgcgtc atggagttcg tgcgcctcgc ctggcgtcgc cgcatccttc
     3061 gtgcccgtgc cgacttgcgg ttgaaacttc cgggcccgag gcgagcgcgg
//
LOCUS       DAOQNN010000385         3105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_22232_length_3105_cov_11.222295, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000385 DAOQNN010000000
VERSION     DAOQNN010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22232_length_3105_cov_11.222295"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3105
                     /gene="hrpA"
                     /locus_tag="PKV97_14960"
     CDS             <1..>3105
                     /gene="hrpA"
                     /locus_tag="PKV97_14960"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HNB07231.1"
                     /translation="LERSRAALRARRAALPVPDFPPELPVSGRRDEIATALAAHQVII
                     VCGETGSGKTTQLPKIAMTLGRGVAGLIGHTQPRRLAARATASRIAQELKSPLGEVVG
                     YKIRFTDKTGERSHVKLMTDGILLAETQTDPLLAAYDTLIIDEAHERSLNIDFLLGYL
                     KTLLPRRPDLKVVVTSATLDAERFARHFADAVGKPAPVIEVSGRLYPIEMRYRPVESE
                     EIDPAAAARSAAKGGREAAKDRSRDLMDALVDAVDEAQRCGPGDVLVFLPGEREIREA
                     AEALRKAHHLPGTEILPLFARQSAQEQARVFSASNGRRVVLSTNVAETSLTVPGIRYV
                     VDTGLARIKRYSPRNKVEQLQVEKIARSAAQQRAGRCGRVMDGICIRLYDEDDFNRRP
                     AHTDPEILRSSLAGVILRMKALKLGAVEDFPFIDAPGARLIGDGYALLAELGAVSDDD
                     ERKLTPTGVELAKLPLDPRIGRMILAARDRGALAELLVIAAALSVQDPRERPQDSPGA
                     ADQAHARFRGGEQDQKSEFLWYWHLWKAWDEVQRHESSSKQKAWCKKHFLNYMRMREW
                     RDVFAQLHTLCAEHGWKENEQPANYEAIHKALLAGLLGNVGCKVEDASGPQAGAYLGA
                     RGIKFWPHPGSALAKKAGKWIMAAEQVETSRLFGRCIARIEPEWVEEVGGHLIKRQVF
                     EPHWSKASGAVRAWERGTLYGLVIYPRRGVAYRDIDPALCRELFIREGLVQGEIAEGP
                     ARAMAFLAHNQRLVAEIERLEHKSRRPDVLVDETLIEAFYDSKLPAEVCDLAGLEAWR
                     KPAEKAEPKLLHLSREQLMRHDAEGVTTDRFPPTLEVLGQKLKLAYLHQPGEADDGVT
                     LAVPLAMLNQIPANRCEWLVPGLLEEKVAALMKTVPQKHRHRLQPVAESAAAFMAVFE
                     AGEFDTDEPLLKMLQRFVEERVQLKLPLESFRPENLKPHCFMNFRVIDEHGRLMGQSR
                     NLMELRARFREQVAARFSAAKIGGALAGALSVVGVGGAGGGQGVGAGGAGVSARGGGS
                     DSVAG"
BASE COUNT          477 a         1077 c         1125 g          426 t
ORIGIN      
        1 gctcgaacgc tcgcgcgccg cgctgcgcgc acgccgcgcc gccttgccgg tgccggactt
       61 cccgcccgag ctgccagtgt ccgggcgccg cgacgagatc gccacggccc tcgccgccca
      121 ccaggtcatc atcgtgtgcg gcgagaccgg ctcgggcaag accacccagc tgccgaagat
      181 cgcgatgacg ctcggccgcg gcgtcgccgg cctcatcggc cacacccagc cgcgtcgcct
      241 ggccgcacgc gccaccgcga gccgcatcgc gcaggagctc aagagcccgc tcggcgaggt
      301 cgtgggctac aagatccgct tcaccgacaa gaccggcgag cgcagccacg tcaagctgat
      361 gaccgacggc attctgctcg ccgagaccca gaccgacccg ctgctcgccg cctacgacac
      421 gctgatcatc gacgaggcgc acgagcgcag cctcaacatc gatttcctgc tcggctacct
      481 gaagaccctg ctgccacgcc ggccggatct gaaggtggtg gtgacctcgg cgacgctgga
      541 tgccgagcgt ttcgcccggc actttgccga tgccgtgggc aagccggcgc cggtgatcga
      601 ggtgtcgggc cggttgtatc cgatcgagat gcgctaccgg ccggtggaga gcgaggagat
      661 tgaccccgcc gcggccgcgc gcagcgccgc caagggtggc agggaggccg ccaaggacag
      721 gagccgcgac ctcatggacg cgctggtcga tgcggtcgac gaggcccagc gctgcggccc
      781 gggtgacgtg ctggttttcc tgcccggcga gcgcgagatc cgcgaggccg ccgaggcgct
      841 gcgcaaggct caccacctgc ccggcaccga gatcctgccg ctgttcgcgc gccagtcggc
      901 gcaggagcaa gcgcgcgtgt tctcggcctc caacggccgc cgggtggtgc tgtcgaccaa
      961 cgtcgccgag acctcgctga ccgtgccggg catccgctac gtggtcgaca ccggactcgc
     1021 gcgcatcaag cgctactcgc cgcgcaacaa ggtcgagcag ctgcaggtcg agaagatcgc
     1081 ccggtcggcc gcgcagcagc gcgccgggcg ctgcggtcgt gtcatggacg gcatctgcat
     1141 ccgcctctac gacgaggacg acttcaaccg ccgcccggcg cacaccgatc ccgagatcct
     1201 gcggtcgtcg ctcgccggcg tgatcctgcg catgaaggcc ctgaagctcg gcgcggtgga
     1261 ggacttcccc ttcatcgacg cccccggcgc gcgcctgatc ggcgatggct acgcgctgct
     1321 ggccgagttg ggcgcggtca gcgacgacga tgaacgcaag ctcaccccga ccggcgtcga
     1381 gctcgccaag ctgccgctcg acccgcgcat cggccgcatg atcctggccg cgcgcgaccg
     1441 tggcgcgctc gccgagttgc tggtgatcgc cgccgcgctg tcggtgcagg acccgcgcga
     1501 gcgcccgcag gacagcccgg gtgccgccga ccaggcgcac gccaggttcc gcggcgggga
     1561 gcaggatcag aagtccgagt tcctgtggta ctggcacctg tggaaggcct gggacgaggt
     1621 ccagcgccac gagtcctcca gcaagcagaa ggcctggtgc aagaagcact tcctcaacta
     1681 catgcgcatg cgcgagtggc gcgatgtgtt cgcccagctc cacaccctgt gcgccgagca
     1741 cggctggaag gagaacgaac agccggcgaa ctacgaggcg atccacaagg cgctgctcgc
     1801 cgggctgctc ggcaacgtcg gctgcaaggt cgaggatgcc tcggggccgc aggccggcgc
     1861 ctacctcggc gcgcgcggga tcaagttctg gccgcacccg ggctccgcgc tggcgaagaa
     1921 ggccggcaag tggatcatgg ccgccgagca ggtcgagacc agccgcttgt tcgggcgctg
     1981 catcgcgcgc atcgagccgg agtgggtgga ggaggtcggc ggccacctga tcaagcgcca
     2041 ggtgttcgag ccgcactggt cgaaggcgtc cggcgcggtg cgcgcctggg agcgcggcac
     2101 gctgtacgga ctggtgatct atccgcgccg cggcgtggcc tatcgcgaca tcgaccccgc
     2161 gctgtgccgc gagctcttca tccgcgaggg cctggtgcag ggcgagatcg ccgaggggcc
     2221 ggcgcgcgcg atggccttcc tcgcccataa ccagcgcctg gtggcggaga tcgagcgcct
     2281 cgagcacaag tcgcgccgcc ccgacgtgct ggtggacgag accctgatcg aggccttcta
     2341 cgacagcaag ctccccgcgg aggtctgcga cctggcgggg ctggaggcgt ggcgcaagcc
     2401 ggcggagaag gccgagccca agctgctgca tctgtcgcgc gagcaactga tgcgccacga
     2461 cgcggaaggg gtgaccaccg accgtttccc gccgacgctg gaggtgctcg ggcagaagtt
     2521 gaagctcgcc tatctgcacc agcctggcga ggccgacgac ggcgtgacgc tcgcggtgcc
     2581 gctggcgatg ctcaaccaga tccccgccaa ccgctgcgag tggctggtgc cgggcctgct
     2641 cgaagagaag gttgcggcgc tgatgaagac cgtgccgcag aagcaccgcc accgcctgca
     2701 gccggtggcc gagagcgcgg cggccttcat ggcggtcttc gaggccggcg agttcgacac
     2761 cgacgagccg ctgctgaaga tgctgcaacg cttcgtggag gagcgcgtgc agttgaagct
     2821 gccgctggag agcttccgcc cggagaacct gaagccgcac tgcttcatga acttccgcgt
     2881 gatcgacgag cacggccgct tgatggggca gtcgcgcaac ctgatggagc tgcgtgcgcg
     2941 cttccgcgag caggtggcgg cgcgcttctc ggcggcgaag atcggcggcg cgctcgcggg
     3001 ggcgctgtcg gtggtgggcg tcgggggcgc gggtggtggc caaggcgtcg gtgccggcgg
     3061 agcgggcgtc agtgcgcggg ggggcggatc cgactccgtt gcggg
//
LOCUS       DAOQNN010000386         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_22261_length_3103_cov_4.855315, whole genome shotgun sequence.
ACCESSION   DAOQNN010000386 DAOQNN010000000
VERSION     DAOQNN010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22261_length_3103_cov_4.855315"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PKV97_14965"
     CDS             complement(<1..980)
                     /locus_tag="PKV97_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HNB07232.1"
                     /translation="MTRPLPAATTLARPVRPSRLCLAVAQVCIVLGMPTAAGNAQAGG
                     GATASVASAQTAIRAGERLSDWLLRNPPGAGDDPAALAWQVPSARQAQAELKQALLAQ
                     LWSARDLQADETTRQRLADWLRSLPVTGRVPLAIVDARWLQANPDQDPILAPGHALVL
                     PQRPGTVTLLADDGRPCAVAHRPGTQARGYLQACIGGAASEADMAWIVQPDGRVQRFG
                     IATWNAEPQSEPAPGAWIWAPRRSAAWPEDFSDRLATFLATQSPTPIAARAAPTQTTT
                     PIAAHAAPTQNGTLATAPVGATPVAIPPSAPTPAAGAHDERLIAAHAAPT"
     gene            complement(985..1665)
                     /locus_tag="PKV97_14970"
     CDS             complement(985..1665)
                     /locus_tag="PKV97_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HNB07233.1"
                     /translation="MLAILACAACSTDSTNISRTFELAYRGGSLADRAAPNPAYRYLR
                     TVVDGRSALLVLGYTDPDPATPDAPPIEVWYSAEGEVVRLQQGRVVGTSGLSTDWRST
                     RAPDAPTWGSLVASAAQDRAAGQVLATWQRTRDESAYRSGVIDRISVHAIAAPRGTEL
                     AGLAPESLQWFEERAEPIVPPAGTPGAVRLTARYALRVDSGATEVVYTEHCLGPELCL
                     SLQRWPAR"
     gene            complement(1956..2264)
                     /locus_tag="PKV97_14975"
     CDS             complement(1956..2264)
                     /locus_tag="PKV97_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07234.1"
                     /translation="MKKTALIAAILAFSTAPVLAQTAPRPGTGAPAQAAPAPAQAGAA
                     GAQAGAAGSGAGAAGASAAAVGGLTAGTVAAVAVGVAAVAAAASSDSSSGTVAPSGTR
                     "
     gene            2508..>3103
                     /locus_tag="PKV97_14980"
     CDS             2508..>3103
                     /locus_tag="PKV97_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNB07235.1"
                     /translation="MNTTEHCNRLRSAAKALILGLALACSSVTMAQNAPAAAGFAAAG
                     GAPAAVGSGGLGMGAPALSGGAPGAAVGAASGAAASAVAADGFETQAAPTVIGGEGLS
                     PSQDAALDQAVTVDADGNDRVGGTVRAARVLNDFQQFVARSTGQVLPLYGSGFFAGSR
                     VFNSPTAPVADDYVLGPGDQVLVRIWGAFESQTRAQIDR"
BASE COUNT          428 a         1004 c         1212 g          459 t
ORIGIN      
        1 tctgtaggag cggcgtgagc cgcgatcagg cgctcgtcgt gcgcaccggc ggcgggcgtg
       61 ggggccgacg gcgggatcgc gactggcgtc gctcctacgg gggcggtagc aagcgtgccg
      121 ttttgtgtag gagcggcgtg agccgcgatc ggcgtggtgg tttgtgtagg agcggcgcga
      181 gccgcgatcg gcgtggggga ctgcgtcgcc aggaaggtgg cgaggcggtc ggagaaatcc
      241 tcgggccagg cggcgctacg gcgcggcgcc cagatccagg cgccgggagc cggttccgat
      301 tgcggttcgg cgttccatgt ggcgattccg aaccgctgca cgcgaccatc tggttgcacg
      361 atccaggcca tgtcggcctc gctggccgct ccgccgatgc aggcctgcag gtagccgcgg
      421 gcctgggtgc cgggccggtg cgcgacagca caagggcggc cgtcgtcggc gagcaaggtg
      481 acggttccgg ggcgctgtgg cagcacgagc gcgtggccgg gggcgaggat cggatcctgg
      541 tccggattgg cctgcagcca gcgtgcgtcg acgatcgcca gcggcacgcg gccggtgacg
      601 ggcaggctgc gcagccagtc ggcgaggcgc tggcgggtgg tctcgtcggc ctgcaggtca
      661 cgtgcagacc acagctgggc gagcaaggcc tgcttgagct ccgcctgggc ctggcgggcg
      721 cttggcactt gccaggcgag tgcagcgggg tcgtcgccag cgccgggcgg gttgcgcagc
      781 agccagtcgc tgaggcgttc gcccgcgcgg atggccgttt gtgcggacgc cacggacgcg
      841 gtcgcgccgc cgccggcctg ggcgttgccg gccgcggtcg gcatccccag cacgatgcag
      901 acctgcgcca cggcgaggca cagacgtgat ggccggacgg ggcgtgcgag cgtggtcgcg
      961 gcgggaagcg ggcgcgtcat cgagtcaccg tgccggccag cgctgcagcg agaggcagag
     1021 ctccgggccg aggcagtgtt cggtgtagac gacctcggtc gcgccgctgt cgacgcgcag
     1081 ggcgtagcgc gcggtgaggc ggacggcgcc cggcgtgccg gcgggcggga cgatcggttc
     1141 ggcgcgctct tcgaaccatt gcaggctttc gggggcgagg ccggcgagct cggtgccgcg
     1201 cggggcggcg atggcatgca cgctgatgcg gtcgatgacc cccgagcggt aggcggattc
     1261 gtcgcgggtg cgttgccagg tggcgagcac ttggccggca gctcggtcct gagctgcgct
     1321 cgcgaccagg ctgccccagg tgggggcgtc tggtgcgcgg gtgctgcgcc agtcggtgct
     1381 gagcccgctg gtgccgacga cgcgcccctg ctgcaggcgc acgacctcgc cctccgcgct
     1441 ataccagacc tcgatcggag gcgcgtcggg cgtcgctgga tcgggatcgg tgtagcccag
     1501 cacgagcagc gcgctgcgac cgtcgaccac cgtgcgcagg tagcgatagg ccggattcgg
     1561 cgcagcccgg tccgccaagg agccgccgcg gtaggcgagc tcgaaggtgc gcgagatgtt
     1621 ggtcgagtcg gtgctgcagg ccgcgcaggc gagaatcgcc agcacggcca gtcgctctgg
     1681 taaagcgcgc gacatcggaa cagaaggtac ggcgttcatc aaacgaaaag cccccaaagg
     1741 gtgacccccg gggggcttgt gcttcgagtc gggtggtggt cgcgcgcagc tgttcgcggc
     1801 ttgcgccgct cctacggggc gcttgggcct tggcgaggtt cgcggcttgc gccgctccta
     1861 caaggcgctg gggccgtggc tttcgtgtag gagcggcgca agccgcgaat tttcagctgt
     1921 tgcgaacgat cgacctgaca acccgaggcg agcgatcaac gcgtgccgga cggtgcaacg
     1981 gtgcccgagc tgctgtcgct gctcgccgcc gcggccacgg ccgccacgcc gactgcgacg
     2041 gcggccaccg tgcctgcggt caaaccaccc accgcagccg cgctggcacc ggcggcgccc
     2101 gctccgctac ccgcagcacc ggcttgcgca ccggccgcac ccgcttgggc cggcgcaggg
     2161 gccgcctggg caggagcgcc cgtgccgggg cggggggcgg tttgcgccag cacgggcgcg
     2221 gtggagaagg caaggatcgc ggcgatcaga gcggtttttt tcatggaaac tcctgtcgag
     2281 gaaagcgcga ccggctggcc gagcgctgga aaagtttgtt tcggacgcaa ctattctagc
     2341 ttgtgccgac cgtggttgaa acatgaacaa gtccttgaag gagaagaata cgacaatcga
     2401 ggccgcccga ttccggctgc cccgccaagc cgtgaccgag cgcagccagt cagccgactt
     2461 tttccagcta caatccacgc cctcaagcgt ccttatgttg tcagaaaatg aataccaccg
     2521 aacattgcaa ccgcctgcgg agcgctgcga aggcgctgat cctgggcttg gcgctggctt
     2581 gctcgtccgt gacgatggcg cagaacgccc ccgcagcagc gggcttcgcg gcggcgggcg
     2641 gtgctccggc tgccgtgggg agcggcgggc tgggcatggg ggcccctgcc ttgtccggtg
     2701 gcgcgccggg tgcggccgtc ggtgccgcca gcggtgctgc ggcatctgca gttgccgcgg
     2761 acggattcga gacccaggct gctccgaccg tgatcggcgg tgaaggcctg agcccctccc
     2821 aggatgccgc gctcgaccag gcggtgacgg tcgacgccga cggcaacgac cgcgtgggcg
     2881 gcacggtgcg cgccgcgcgg gtgctcaacg acttccagca attcgtggcg cgcagcaccg
     2941 ggcaggtgct gccgctgtac ggttcgggct tcttcgcggg ctcgcgagtg ttcaacagcc
     3001 ccaccgcgcc ggtcgccgac gactacgtgc tcggcccggg cgaccaggtg ctggtgcgga
     3061 tctggggcgc cttcgagtcg cagacccggg cgcagatcga ccg
//
LOCUS       DAOQNN010000387         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_22328_length_3098_cov_9.005587, whole genome shotgun sequence.
ACCESSION   DAOQNN010000387 DAOQNN010000000
VERSION     DAOQNN010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22328_length_3098_cov_9.005587"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..564
                     /gene="uraD"
                     /locus_tag="PKV97_14985"
     CDS             46..564
                     /gene="uraD"
                     /locus_tag="PKV97_14985"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HNB07236.1"
                     /translation="MDAALDELSRLAQDAFVARLEGIFEHSPWIAERAWSAGPFASID
                     ALHAAMCLVVDTAAPAEQLGLICAHPELAGKEAEEGTLTTASTGEQRGAGLDQCSKEE
                     LQRLRRLNQDYRARFGFPFVVAVKGLTRHQILDRVEARLANARDAEFRTCLAEIGKIA
                     RFRLDAMFGTNS"
     gene            891..1823
                     /gene="puuE"
                     /locus_tag="PKV97_14990"
     CDS             891..1823
                     /gene="puuE"
                     /locus_tag="PKV97_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HNB07237.1"
                     /translation="MTSNPTYPRDLVGYGRNPPHADWPGRARIAVQFVLNYEEGGEHC
                     VLHGDAGSEQFLSELFNAASYPDRHLSMEGIYEYGSRVGVWRFLREFERRGLPLTIFG
                     VSMALERHPELTAAFKELGHEIACHGWRWIHYQNVPEEIEREHMKIGMEIIERLTGTR
                     ALGWYTGRDSPNTRRLVADHGGFLYDSDYYGDDLPLWTEVRRTDGETVPHLVVPYTLD
                     TNDMRFALPQGFSHGDEFFEYLRDAFDVMYAEGEERPAMMSIGMHCRLLGRPGRFRAL
                     QRFLDHIEKHDRVWVCRRVDVARHWIERHPYQAK"
     gene            1925..2974
                     /gene="alc"
                     /locus_tag="PKV97_14995"
     CDS             1925..2974
                     /gene="alc"
                     /locus_tag="PKV97_14995"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HNB07238.1"
                     /translation="MASHTPGAPVFAPAAELPDWALRSVDLANPRLGAKAIAASDDFF
                     AEVGRMLNPEPAQFVPGKFDTNGKWMDGWESRRKRVAGHDWALVKLGVKGVIRGFDVD
                     TSHFTGNYPPAVSIEACVSETDDVAALQAAEWTEILPASPMGPNSHHLLECASTAAWT
                     HLRVNMYPDGGIARLRVYGRPVGTLAAKAAGGELVDLVAMENGGRALSWNDASFGSSA
                     AALLLPGRGMNMGDGWETRRRREPGNDWCVLELGAPGTVERIEVDTAFFKGNYPDRCS
                     VQAAYVSGGTDRSITTQSMFWQTLLPEQKLEMDAIHTFTEQVAKLGPITHVRFNIFPD
                     GGVSRLRLWGKVEAK"
BASE COUNT          498 a         1196 c          981 g          423 t
ORIGIN      
        1 cccgccaccg ccccaagcac cgcccccgac cgcccggccg ccgcgctgga cgccgccctg
       61 gatgaactct cccgcctggc gcaggacgcc ttcgtcgccc gcctcgaagg catcttcgag
      121 cactcgccgt ggatcgccga gcgtgcctgg agcgccggtc ccttcgccag catcgacgcg
      181 ctgcacgccg cgatgtgcct ggtggtcgac accgccgccc ccgccgagca gctcggcctc
      241 atctgcgccc accccgagct cgccggcaag gaagccgagg aaggcacgct gaccacggcc
      301 tccaccggcg agcagcgcgg cgccgggctc gaccagtgca gcaaggaaga gctccagcgc
      361 ctgcgccggc tcaatcagga ctaccgcgcg cgcttcggct ttcccttcgt ggtcgcggtc
      421 aagggcctca cccgccacca gatcctggac cgcgtcgagg cccgcctggc caacgcgcgc
      481 gacgccgaat tccgcacctg cctggccgag atcggcaaga tcgcccgctt ccggctggat
      541 gcgatgttcg ggacgaacag ctgaacggcc gccgcgaggg cgcgggtggt cagcatctcg
      601 gcgcgggaat cgcgactcgc gtcgctcctg caggaaccac cccgccaacg cagccgcacg
      661 gtaggagcga cgcaagtcgc gatcaaacgc gcgtcgcgcc tagcgcgcgg cgaggcaggc
      721 gaggtcggtt tcgtgagagc gacgcaagtc gcgatcaaac gcccccgcct gtgcgaagcc
      781 gacattcggc gccaagtcat cgcccggatc gcgactggcg tcgctcccac acgaaccgcc
      841 acggccgatt ggcctgttga atccctgatc gcctgtctgg acagaacacc atgacctcga
      901 acccgaccta cccccgcgac ctcgtcggct acggccgcaa cccgccccac gccgactggc
      961 caggccgcgc ccgcatcgcg gtgcagttcg tgctcaacta cgaggaaggc ggcgagcact
     1021 gcgtgctgca cggcgacgcc ggcagcgagc agttcctctc cgagctcttc aatgccgcga
     1081 gctaccccga ccgccacctg tcgatggagg gcatctacga gtacggctcg cgcgtgggcg
     1141 tttggcgctt cctgcgcgag ttcgagcgcc gcggcctgcc gctgacgatc ttcggcgtgt
     1201 cgatggcgct cgagcgccac cccgaactca ccgccgcctt caaggaactc ggccacgaga
     1261 tcgcctgcca cggctggcgc tggatccact accagaacgt gcccgaggag atcgagcgcg
     1321 agcacatgaa gatcggcatg gagatcatcg agcgcctcac cggcacgcgc gcactgggct
     1381 ggtacaccgg ccgcgactcc ccgaacaccc gccgcctggt cgccgaccac ggcggcttcc
     1441 tgtacgactc ggactattac ggcgacgacc tgccgttgtg gaccgaggtg agaaggaccg
     1501 acggcgagac cgtgccgcat ctggtggtgc cctacacgct cgacaccaac gacatgcgct
     1561 tcgcgctgcc gcagggcttc tcgcacggcg acgagttctt cgaatacctg cgtgacgcct
     1621 tcgacgtgat gtacgccgag ggcgaggagc gtcccgcgat gatgtcgatc ggcatgcact
     1681 gccgcctgct cggccgcccg ggccgcttcc gcgccctgca gcgcttcctc gaccacatcg
     1741 agaagcacga ccgcgtgtgg gtgtgccgtc gcgtcgacgt cgcccgccac tggatcgagc
     1801 gccaccccta ccaagcgaag taagccgggg ggaaatcggg aaatcgggga cagaccacga
     1861 tttcccgagc gactcccggc tctttcccgc ctgcctcacg acccgaaccc aaaggagaac
     1921 ccccatggcc agccacaccc ccggcgcccc cgtcttcgcc cccgctgccg agctgcccga
     1981 ctgggcgctg cgctcggtcg acctcgccaa cccgcgcctc ggcgccaagg cgatcgccgc
     2041 ctccgacgac ttcttcgccg aagtcggccg catgctcaac cccgaacccg cgcagttcgt
     2101 ccccggcaag ttcgacacca acggcaagtg gatggacggc tgggagtcgc gcaggaagcg
     2161 cgtcgccggc cacgactggg cgctcgtgaa actcggcgtc aagggcgtga tccgcggctt
     2221 cgacgtcgac acctcccact tcaccggcaa ctacccgccc gcggtgtcga tcgaggcctg
     2281 cgtgtccgag accgacgacg tcgccgccct gcaggcggcc gagtggaccg agatcctccc
     2341 cgccagcccg atgggcccca acagccacca ccttctcgag tgcgccagca ccgcggcgtg
     2401 gacccacctg cgcgtgaaca tgtaccccga cggcggcatc gcgcgcctgc gcgtctacgg
     2461 ccgcccggtg ggcacactcg cggccaaggc cgccggcggc gagctggtcg acctggtggc
     2521 aatggagaac ggcggccgcg cgctgtcgtg gaacgacgcc agcttcggct catccgccgc
     2581 cgccctgctg ctgcccgggc gcggcatgaa catgggcgac ggctgggaga cccgccgccg
     2641 ccgcgagccc ggcaacgact ggtgcgtgct cgagctcggc gcccccggca cggtcgagcg
     2701 catcgaggtc gacaccgcct tcttcaaggg caactacccg gaccgctgct cggtgcaggc
     2761 cgcctacgtc agcggcggca ccgaccgctc gatcaccacc cagtcgatgt tctggcagac
     2821 cctgctgccc gagcagaagc tggagatgga cgcgatccat acctttacgg agcaggtcgc
     2881 gaaactgggg ccgatcaccc acgtccgctt caacatcttc cccgacggcg gcgtctcgcg
     2941 cctgcgcctg tggggcaagg tcgaggcgaa gtgagaccgg cggcggccgc gcccgcgccg
     3001 ccgcggatgc ccgcgccgac catcgcgact tgcatcgctc ctgcccgtgc tgccgagacc
     3061 gccaatcgcg acttgcgtcg ctcctacccg tgctgccg
//
LOCUS       DAOQNN010000388         3095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_22375_length_3095_cov_10.790132, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000388 DAOQNN010000000
VERSION     DAOQNN010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22375_length_3095_cov_10.790132"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PKV97_15000"
     CDS             <1..784
                     /locus_tag="PKV97_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNB07239.1"
                     /translation="GQCCPQPAPEPRPEPKPEPKPEPKPEPKPEPKPEPKPEPKPEPR
                     PEPPKPQAKPEIVAKAPEKKPEPPKPEPPKPEPKPQPKPEPKPEPKPEPKPQPKPEPK
                     PEPKPQAKPEPKPQPKPDTKPIDDYMAQRLAQETQRAEQARLSSLMAQEGARAAAAGP
                     SRQGPPGGDIDKYRAAIAAKVRGNLLRPPGLSGNPEAVFEVDQLPSGEVLNVRLKRSS
                     GVPALDEAIERAIRRSSPLPLPDNKNQFERTLELKFRPLADD"
     gene            825..2102
                     /gene="tolB"
                     /locus_tag="PKV97_15005"
     CDS             825..2102
                     /gene="tolB"
                     /locus_tag="PKV97_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNB07240.1"
                     /translation="MIKTLHAFRLFLAGLLASLALGAHAQLSIEITGTGASRFPVIIP
                     IFENEGRLPQSVTDVVRADLERSGLFTLIDTGPLPMPESVAPDLAAVRSRGADAVLTG
                     SLIPMADGRYDVRFRLFDAQKQQELGAMSLPMSPAQNRLVGHRIADFVYEKLTGLPGY
                     FATRIAYVIKSGPNFELQIADADGMNAATALRSREPIISPSWSPDGQRLAYVSFEQKK
                     PIVYVHTLATGQRRVVANFKGSNSAPSWSPDGSQLAVVLTKDGLSQLYAIGADGSGVR
                     RLAQSSGIDTEPFWGADGHIYFTSDRGGSPQIYRIPASGGSAQRVSFEGNYNVTPRLS
                     FDGKLLAFITRNAGRFQVAVQDVATRQTTILTDSARDESPSFAPNGRMILYATDTGGR
                     GVLSAVSTDGRIRQRLTVQAADVREPAWGPQIR"
     gene            2171..2683
                     /gene="pal"
                     /locus_tag="PKV97_15010"
     CDS             2171..2683
                     /gene="pal"
                     /locus_tag="PKV97_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNB07241.1"
                     /translation="MKKLVLPALLALTLAACSSTGPENGAASVQDRGTGVATVTAGSA
                     KGSGIAALTDPNNILSKRSVFFDFDSFVIKQEARPLIEAHARFLVQNPQMNMLVQGNT
                     DERGSREYNLALGQKRADVVKQALMLLGAKESQIESVSLGEEKPRCEDRTENCYAENR
                     RGDMLYTGEF"
     gene            2696..>3095
                     /locus_tag="PKV97_15015"
     CDS             2696..>3095
                     /locus_tag="PKV97_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgF trimerization domain-containing protein"
                     /protein_id="HNB07242.1"
                     /translation="MKRLLPLAAAFVLIPSAPAHAQLFGGDTEARNQIFQLRQDVQGQ
                     IETTSRGQLELAMQNEQMRAELARLRGQIELLTNEVETLKQRQKDFYVDLDARLRRYE
                     SGAAGAASAAPASGDAAPAAPAATAAPPQAP"
BASE COUNT          568 a         1209 c          929 g          389 t
ORIGIN      
        1 cggccagtgc tgcccgcagc cggcgccgga gccccggccg gagccgaaac ccgaaccgaa
       61 gcccgagccc aaacccgaac cgaagcccga gcccaagccg gaaccgaagc cggaaccgaa
      121 gccggaaccc agacctgaac cgcccaagcc ccaggccaag cccgagatcg tcgccaaggc
      181 gcccgagaag aagccggagc cgcccaagcc cgagccaccg aaacccgaac ccaaaccaca
      241 accgaagccg gagcccaagc ctgaaccgaa gcccgagccc aagccccaac ccaagccgga
      301 gccgaaaccc gagcccaagc cgcaggccaa gcccgaaccc aagccccagc ccaagccgga
      361 caccaagccg atcgacgact acatggcgca gcgcctcgcg caggagaccc agcgcgccga
      421 gcaggcccgc ctgtccagcc tgatggcgca ggaaggtgcg cgcgcggccg cggccggtcc
      481 gagccgccag ggtccgcccg gtggcgacat cgacaaatac cgcgcggcga tcgctgccaa
      541 ggtgcgcggc aacctgttgc gcccgcccgg cctgtccggc aacccggagg cggtgttcga
      601 ggtcgaccag ctgccgagtg gcgaagtgct caatgtccgc ctcaagcgct cgtccggcgt
      661 gcccgccctc gacgaggcca tcgagcgcgc gatccggcgc tcgagcccgc tgccgctgcc
      721 ggacaacaag aaccagttcg agcgtaccct cgaactgaaa ttccgtccgc tggcggacga
      781 ttgagtccgt catgtctata atccgcggct ggagaaaacc ccgcatgatc aaaacactgc
      841 acgcattccg cctcttcctc gccggcctgc tcgccagcct cgcgctgggt gcgcacgccc
      901 agctctcgat cgagatcacc ggcaccggcg cctcgcgctt tccggtcatc atcccgatct
      961 tcgagaacga gggccgcctg ccgcagagcg tcaccgacgt cgtgcgcgcc gacctcgagc
     1021 gcagcggtct gttcaccctg atcgacaccg gcccgctgcc gatgcccgaa tcggtggcgc
     1081 ccgacctcgc cgcggtgcgc agtcgcggcg ccgacgcggt gctcaccggc agcctgatcc
     1141 cgatggccga cgggcgctac gacgtgcgct tccggctctt cgacgcgcag aagcagcagg
     1201 aactcggcgc catgtcgctg ccgatgagtc cggcgcagaa ccgcctggtc ggccaccgca
     1261 tcgccgactt cgtgtacgag aagctcaccg gcctgccggg ttacttcgcc acccgcatcg
     1321 cctacgtgat caagtccggt cccaacttcg agctccagat cgccgacgcc gacggcatga
     1381 acgccgccac cgcgctgcgc tcgcgcgagc cgatcatctc gccttcgtgg tcgcccgacg
     1441 gccagcgcct ggcctatgtg tccttcgagc agaagaagcc gatcgtctac gtgcacacac
     1501 tcgccaccgg ccagcgccgc gtggtcgcca acttcaaggg ctcgaactcc gcgccgagct
     1561 ggtcgcccga cggcagccag ctcgcggtcg tgctcaccaa ggacggcctg tcgcagctct
     1621 acgcgatcgg tgccgacggc tccggcgtgc gccgcctcgc gcaatcctcc ggcatcgaca
     1681 ccgagccctt ctggggcgcc gacggccaca tctacttcac ctcggatcgc ggcggcagtc
     1741 cgcagatcta ccgtattcct gccagtggcg gcagcgccca gcgggtgagc ttcgaaggca
     1801 actacaacgt gacgccgcgc ctgtcgttcg acggcaagtt gctcgccttc atcacccgca
     1861 atgccggccg cttccaggtg gccgtgcagg acgtcgccac ccggcagacc accatcctca
     1921 ccgattcggc acgcgacgaa tcgcccagct tcgcccccaa cggccgcatg atcctctacg
     1981 ccaccgacac cggtggccgt ggcgtgctgt cggcggtgtc gaccgacggg cgcatccgcc
     2041 agcggctcac ggtgcaggcc gccgatgtcc gcgaacccgc ctggggtccg cagatccggt
     2101 aaccatgaag cggcaacaaa gccgcatacc cctctctgct gtcatcaaca ctcgtactgg
     2161 agcatcaccc atgaagaaac tcgtcctccc cgcgctgctc gccctgaccc tggccgcctg
     2221 ctcgagcacc ggccccgaga acggcgccgc ctccgtccag gaccgcggca ccggcgtcgc
     2281 caccgtgacg gccggcagcg ccaagggcag cggcatcgcg gcgctcaccg accccaacaa
     2341 catcctctcc aagcgcagcg tcttcttcga cttcgacagc ttcgtgatca agcaggaagc
     2401 ccgcccgctg atcgaagccc acgcccgctt cctggtgcag aacccgcaga tgaacatgct
     2461 cgtccagggc aacaccgacg agcgcggcag ccgcgaatac aacctcgccc tcggccagaa
     2521 gcgcgccgac gtcgtcaagc aggccctcat gctgctcggc gccaaggaat cgcagatcga
     2581 gtccgtgagc ctgggtgaag agaagccgcg ctgcgaagac cgcaccgaga actgctacgc
     2641 cgagaaccgc cgcggcgaca tgctctacac gggcgagttc taagagagtc ccgacatgaa
     2701 acgccttctg ccgctcgccg cagcgttcgt cctcatcccg agcgccccgg cgcacgccca
     2761 gctcttcggg ggtgacaccg aggcccgcaa ccagatcttc cagctccggc aggacgttca
     2821 ggggcagatc gagaccacca gccgcggcca gctcgagctc gccatgcaga acgaacagat
     2881 gcgcgccgag ctggcgcgcc tgcgtggcca gatcgaactg ctgaccaacg aggtggagac
     2941 gctcaagcag cgccagaagg atttctacgt cgacctcgac gcccgcctgc gccggtacga
     3001 atccggcgcg gcgggcgccg cctccgccgc cccggcctcc ggggatgcgg cgccggcggc
     3061 accggctgcg accgcagcgc ccccgcaggc acctg
//
LOCUS       DAOQNN010000389         3073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_22655_length_3073_cov_11.833996, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000389 DAOQNN010000000
VERSION     DAOQNN010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22655_length_3073_cov_11.833996"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PKV97_15020"
     CDS             complement(<1..698)
                     /locus_tag="PKV97_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HNB07243.1"
                     /translation="MNLQQLRYVHEVARRGLNVSEAADALFTSQPGVSKQIRQLESEL
                     GIEIFTRHGKRLVAVTEPGRAVLAIAERVLRDLDNLQQVGDEFANEAVGRLSIATTHT
                     QARYVLPDVIRRFMQRYPQVKLELHQGNPRQVCEMVLDGSADIAIATEAIAEYDELVM
                     LPCYQWNRCVVATPRHPILQAAPLTLEAIARYPLITYDDAFTGRGQINKAFLGRGLKP
                     KVVLTAIDSDVIKK"
     gene            803..1585
                     /locus_tag="PKV97_15025"
     CDS             803..1585
                     /locus_tag="PKV97_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB07244.1"
                     /translation="MDFAYTIAGFAVGAIVGLTGVGGGSLMTPLLVLMFGIHPSVAVG
                     TDLLYAAITKAGGTLAHGLKGTVDWKITRLLATGSIPAAAITLALIGHFAPGGIEGAA
                     HLIQVSLGVALVLTAVALIFRKRIQAYAATRSGGRPADPACTARLTVLTGAVLGVLVS
                     ISSVGAGALGVTALFFLYPAMPALRIVGSDIAHAVPLTAVAGIGHWFLGSVDWLLLGS
                     LLVGSLPGIWLGSHISTKVPDRVLRPILATMLVLVGAKLMAH"
     gene            1654..>3073
                     /locus_tag="PKV97_15030"
     CDS             1654..>3073
                     /locus_tag="PKV97_15030"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNB07245.1"
                     /translation="MYQYDEHDQRLLDQRVAQFRDQMRRHLAGELSADEFRPLRLQNG
                     LYIQRHAPMFRISVPYGHLASRQLRKLAHLARSYDHGYAHFTTRTNVQFNWPKLEDVP
                     EMLAELASVQMHANQTSGNCIRNITADPFAGVAADEVADPRPFAEILRQWATYNPEFA
                     FLPRKFKIAFNSAAEDRVVLRVYDIGLDLVRNEAGELGFRVLVGGGLGRTPILGEEIK
                     PFLPWQHLISYCEAILRVYNRWGRRDNLWKARIKILVKALGPEEFGRQVEEEWANGKD
                     GPNTITAAELARVSAHFAPPAYETLPAQDAAFDELLAGNKAFATWVKRNVRTHRQPGY
                     AAVVLSLKKTGTAPGDVTAEQMDLIADLADRFSFGEARMLHEQNVALADVRRTDLFEL
                     WQAARAAGLATPNIGLLTDIICCPGGDFCSLANARSIPIADAIQRRFDDLDYQHDIGE
                     IDLNISGCMNSCGHHHVGHIGIL"
BASE COUNT          494 a         1127 c          969 g          483 t
ORIGIN      
        1 tacttcttga tcacgtccga gtcgatcgcg gtgagcacca ccttgggctt gaggccgcga
       61 ccgaggaagg ccttgttgat ctggccgcgg ccggtgaagg cgtcgtcgta ggtaatcagc
      121 ggatagcgcg cgatcgcctc gagcgtgagc ggcgcggcct gcaggatggg gtggcgcggg
      181 gtggcgacca cgcagcggtt ccactggtaa cagggcagca tcaccagctc gtcgtactcg
      241 gcgatggcct cggtggcgat cgcgatgtcg gcgctgccgt cgagcaccat ctcgcaaacc
      301 tggcgcggat tgccctggtg cagctcgagc ttgacctgcg ggtagcgctg catgaagcgg
      361 cggatcacgt cgggcagcac gtagcgcgcc tgggtatggg tggtcgcaat cgacaggcgg
      421 cccacggcct cgttggcgaa ttcgtcaccc acctgctgca ggttgtcgag gtcgcgcagc
      481 acgcgctcgg cgatcgccag caccgcccgc cccggctcgg tgaccgccac caggcgcttg
      541 ccgtggcgag tgaagatctc gatgccgagc tcggactcga gctggcggat ctgcttggac
      601 acccccggct gcgaggtgaa cagcgcgtcg gccgcctcgg agacattgag cccgcgacgg
      661 gcgacttcat gcacgtaacg cagttgctgc aggttcatgg cgcgctagat ataacaacca
      721 gttcttaaag tctaactgca aaccgcttct tgcgcttaac tgaagtcctt acgatgcgcc
      781 gcaacatcca ggagcgcgtt acatggactt cgcctacacc atcgccgggt tcgcagtcgg
      841 tgccatcgtc ggtctgaccg gcgttggagg cgggtcattg atgacgccgc tgctggtgct
      901 gatgttcggc atccacccct cggtcgccgt cggcacagac ctgctctacg ccgcgatcac
      961 caaggccggc ggcaccctgg cgcacggact caagggcacg gtggactgga agatcacccg
     1021 cctgctcgcc accggcagca ttcccgcggc ggcgatcacg ctggcgctga tcggacactt
     1081 cgcgcccggc ggcatcgaag gcgcggccca cctgatccag gtctcgctcg gcgtcgcctt
     1141 ggtgctgacc gcggtggcgc tgatcttccg caagcgcatc caggcctatg ccgcgacgcg
     1201 ctcgggtggc cgccccgccg atccggcgtg caccgcccgc ctgaccgtgc tcaccggcgc
     1261 cgtgctcggc gtgctggtat cgatctcctc ggtgggcgcc ggcgcgctcg gcgtcaccgc
     1321 gctgttcttc ctctacccgg cgatgccggc gctacgcatc gtcggctcgg acatcgccca
     1381 cgccgtgccg ctgaccgcgg tcgccggcat cggccactgg tttctcggca gcgtcgactg
     1441 gctgctgctg ggcagcctgc tggtcggctc gctgcccggc atctggctcg gcagccacat
     1501 ttccaccaag gtacccgacc gcgtcctgcg cccgatcctg gcgacgatgc tggtgctggt
     1561 cggcgccaag ctgatggcgc actgagccgc agcgaccaag cacgcgacgc ccctccccgg
     1621 cccgaatcca cgcatacgac agagagagcc catatgtacc aatacgacga acacgaccag
     1681 cgcctgctcg accagcgcgt cgcccagttc cgcgaccaga tgcggcgtca cctggccggc
     1741 gagctctcgg ccgacgagtt ccgtccgctg cgcctgcaga acgggttgta catccagcgc
     1801 cacgcgccga tgttccgcat ctcggtgccc tacggccacc tcgccagccg ccagctgagg
     1861 aagctcgccc acctcgcgcg cagctacgac cacggctacg cccacttcac cacccgcacc
     1921 aacgtgcagt tcaactggcc gaagctggaa gacgtgccgg agatgctcgc cgaactggcc
     1981 agcgtgcaga tgcacgccaa ccagacctcg ggcaactgca tccgcaacat caccgccgac
     2041 cccttcgccg gcgtggccgc cgacgaggtc gccgatccgc gccccttcgc cgagatcctg
     2101 cgccagtggg cgacctacaa cccggagttc gccttcctgc cgcgcaagtt caagatcgcc
     2161 ttcaacagcg ctgccgaaga ccgcgtggtg ctgcgggtgt acgacatcgg cctcgacctg
     2221 gtgcgtaacg aggccggcga gctcggcttc cgcgtgctgg tgggcggcgg cctcggccgc
     2281 accccgatcc tgggcgagga gatcaagccc ttcctgccct ggcagcacct gatcagctac
     2341 tgcgaggcca tcctgcgcgt ctacaaccgc tggggccgcc gcgacaacct gtggaaggcg
     2401 cgcatcaaga tcctcgtcaa ggcgctcggg ccggaagagt tcggccgcca ggtggaggaa
     2461 gaatgggcca acggcaagga cggccccaac accatcaccg cggcggagct ggcgcgcgtg
     2521 tcggcccact tcgccccgcc tgcctacgag accctgcccg cgcaggacgc cgccttcgac
     2581 gaactcctcg ccggcaacaa ggccttcgcc acctgggtca agcgcaacgt gcgcacccac
     2641 cgccagcccg gctacgcggc ggtggtcctg tcgctgaaga agaccggcac cgccccgggc
     2701 gacgtcaccg ccgagcagat ggacctgatc gccgatctcg ccgaccgctt cagcttcggc
     2761 gaggcgcgca tgctgcacga gcagaacgtc gcgctcgccg acgtgcgccg caccgacctc
     2821 ttcgaactgt ggcaggcggc gcgtgccgcc ggcctggcga cgccgaacat cggcctgctg
     2881 accgacatca tctgctgccc cggcggcgac ttctgcagcc tcgccaacgc acgctcgatc
     2941 ccgatcgccg acgccatcca gcgccgcttc gacgacctcg actaccagca cgacatcggc
     3001 gagatcgacc tcaacatttc cggctgcatg aactcctgcg gccaccacca cgtcggccac
     3061 atcggcatcc tcg
//
LOCUS       DAOQNN010000390         3051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_22946_length_3051_cov_6.978638, whole genome shotgun sequence.
ACCESSION   DAOQNN010000390 DAOQNN010000000
VERSION     DAOQNN010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22946_length_3051_cov_6.978638"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1525)
                     /locus_tag="PKV97_15035"
     CDS             complement(89..1525)
                     /locus_tag="PKV97_15035"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNB07246.1"
                     /translation="MTYPHTRLLIDGQWCDALDGRTLAVHNPATGEEIGRVAHAAIAD
                     LDLAVAAAVKGFQTWRRTPAIERAKTLRRAAALMRERAGDIARLLTQEQGKPLPEAKM
                     ETLAAADIIEWFADEGLRVYGRIVPGRNLAATQMVIKDPVGPVAAFTPWNFPINQVVR
                     KAAAALATGCSILVKAAEETPAAPAELVRAFVDAGVPAGVIGLVYGTPAEISSYLIAH
                     PAIRKITFTGSTPVGKQLAALAGQHMKRVTMELGGHAPVIVCEDADLELAIKVSAASK
                     FRNAGQVCISPTRFLVHEAVRETFAAALARHASTLKVGDGLAEGTQMGPLANPRRVAA
                     MADFVQDALARGATVAAGGERIGAAGNFFAPTVLTGVPLDAKVFNEEPFGPVAAIRGF
                     TTLDEAIAEANRLSFGLAGYAFTRSLKNAHRLAHELEVGMLYVNQPATPSAEMPFGGI
                     KDSGYGTEGGPEALDAYLNTRAVTVMNV"
     gene            complement(1972..3048)
                     /locus_tag="PKV97_15040"
     CDS             complement(1972..3048)
                     /locus_tag="PKV97_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNB07247.1"
                     /translation="MLCRTNLLAALATPAPLAEPPAFAPTPDAGWRRFELTSRVEPLP
                     GDGPLRVWVPLPAMHETAWQRPMGSLWQGNADLMERVRDRSGAEMVYAEWAPGIAQPR
                     LEILSRFATRDRAIDFSQPSAAPQVLPRAERMHYLRATALLPTDGIVRDTARDIVHGA
                     KTDEDKARAIYEWIVDNTFREPKVRGCGIGDIRTMLETGNLAGKCADLNALFVGLARA
                     AGLPARDVYGLRVADSRFGYKSLGKSGNVSKAQHCRAEVFLERFGWVPVDPADVRKVV
                     LEEPPGKLSMVDPKVAAVRKQLFGAWEMNWLAYNDAHDLRLPGSTGSEIPFLMYPQGE
                     LAGQRFDSLDPDAFSYTLSAREIA"
BASE COUNT          386 a         1029 c         1157 g          479 t
ORIGIN      
        1 atgaaagtta acgagttatt gatttgagct atgcgaagtc ggtgcgattg ccctgagccc
       61 tgcgccgtca ccgcattgcg gcagcgactt acacgttcat caccgtcacc gcgcgcgtgt
      121 tcaggtaggc gtcgagcgcc tccggcccgc cctcggtgcc gtagcccgaa tccttgatgc
      181 cgccgaaggg catctccgcg ctcggcgtcg cgggttggtt cacgtacagc atgccgacct
      241 cgagttcgtg cgccaggcgg tgcgcgttct tcagcgagcg cgtgaacgcg tagcccgcca
      301 gcccgaagga caggcggttc gcctccgcga tcgcctcgtc cagcgtggtg aagccgcgga
      361 tcgccgcgac cggcccgaag ggttcctcat tgaacacctt cgcgtccagc ggcacgccgg
      421 tcagcaccgt gggcgcgaag aagttgcccg cggcgccgat gcgctctccg cccgccgcca
      481 cggtcgcgcc gcgagccagc gcgtcctgca cgaagtccgc catcgccgcc acgcggcggg
      541 gattggccag cggacccatc tgcgtgccct cggccaggcc gtcgccgacc ttcagcgtgc
      601 tcgcatgccg ggccagcgcg gcggcgaagg tctcgcgcac cgcctcgtgc acgaggaaac
      661 gcgtgggcga gatgcatacc tggccggcgt tgcggaactt cgacgccgcc gacaccttga
      721 tcgccagctc gaggtcggcg tcctcgcaca cgatcaccgg cgcatggccg ccgagctcca
      781 tcgtcacccg cttcatgtgc tggccggcga gcgccgcgag ctgcttgccg accggggtgg
      841 agccggtgaa ggtgatcttg cggatcgccg ggtgggcgat caggtagctc gagatctccg
      901 ccggggtccc atacaccagt ccgatcacgc ctgccggcac gccggcatcg acgaaggcgc
      961 gcaccagctc ggccggcgcc gcgggcgtct cctcggcggc cttgaccagg atggagcagc
     1021 cggtggcgag cgccgccgcg gccttgcgca cgacctggtt gatggggaag ttccacggcg
     1081 tgaaggcggc gaccgggccg accgggtcct tgatcaccat ctgcgtggcg gcgaggttgc
     1141 gtcccggcac gatgcggcca tacacgcgca ggccttcgtc ggcaaaccac tcgatgatgt
     1201 cggcggcggc gagtgtttcc attttcgcct cgggcagcgg cttgccttgt tcctgggtga
     1261 gcaggcgggc gatgtcgccg gcgcgctcgc gcatcagcgc tgcggcgcgg cgcagggtct
     1321 tggcgcgctc gatcgccggg gtgcggcgcc aggtctggaa gcccttcacg gcggcggcga
     1381 cggcgaggtc gaggtcggcg atcgcggcat gcgcgacgcg gccgatctcc tcgccggtgg
     1441 ccgggttgtg cacggccagc gtgcggccgt cgagggcgtc gcaccattgg ccgtcgatga
     1501 gcaggcgggt atgggggtag gtcatggggg cttcctctcg tgggaatgtg ggacagggca
     1561 ttctagcggg tggcggatga cggaagcgcg cacgggcgcg ccgggtcggg ccccgtccgt
     1621 ggccgcggtg tcgggcgccg cgcggggcgt acactcgcga cccttcgctg gcgctcaggg
     1681 ctgccctcgg tggaggacgc cgcgggggcg gcgacgcaaa ctcgtcgctg cgtgcctcgg
     1741 acccgcgtcg ccttgtttcc cccgcgacgt cctccacctc ggcgctccag atcttcccgc
     1801 gcggcacccg atgccgcggc cgggaccgtt tgcgggggct tgcaggcacg gggggccgcg
     1861 tggcctgcag cgctcgcgca ggtgcggggg cttcgatgcc gcaggctgcg gcgcccggcc
     1921 gagggctcgg gatcgcgcga ggtttcgcgc tcgcacgcct gtgttcccgc ctcaggcaat
     1981 ttcccgtgcg ctcagcgtgt agctgaaggc gtcggggtcc aggctgtcga agcgctggcc
     2041 ggcgagttcg ccctgggggt acatcaggaa ggggatctcg ctgccggtgc tgccgggcag
     2101 gcgcaggtcg tgggcgtcgt tgtaggccag ccagttcatc tcccaggcgc cgaagagctg
     2161 cttgcgcacc gcggcgacct tgggatcgac catggagagc ttgcccggcg gttcctccag
     2221 gacgaccttg cgcacgtccg cggggtccac cggcacccag ccgaagcgct ccaggaagac
     2281 ctcggcgcgg cagtgctggg ccttggagac gttgccgctc ttgcccaggc tcttgtagcc
     2341 gaagcgcgag tcggcgacgc gcaggccgta gacgtcgcgt gccggcagcc cggctgcgcg
     2401 cgccaggccg acgaagaggg cgttgaggtc ggcgcacttg cccgcgaggt tgccggtctc
     2461 cagcatcgtg cggatgtcgc cgatgccgca gccgcgcacc ttgggctcgc gaaaggtgtt
     2521 gtccacgatc cattcgtaga tggcgcgcgc cttgtcctcg tcggtcttcg cgccgtggac
     2581 gatgtcgcgt gcggtatcgc gcacgatgcc gtcggtgggc agcagcgcgg tggcgcgcag
     2641 gtagtgcatg cgttcggcgc gcggcagtac ctggggcgcg gcgctgggct ggctgaagtc
     2701 gatggcgcgg tcgcgggtgg cgaagcggct gaggatctcg agccggggct gggcgatgcc
     2761 cggcgcccac tcggcgtaga ccatctcggc gccggagcgg tcgcgcacac gttccatgag
     2821 gtcggcgttg ccctgccaca ggctgcccat cgggcgctgc caggcggtct cgtgcatcgc
     2881 cggcagcggc acccacacgc gcagcggacc gtcgccgggc aggggttcga cgcggctcgt
     2941 caactcgaag cggcgccagc cggcgtccgg cgtgggcgcg aaggcggggg gctcggcgag
     3001 cggggcgggg gtggcgaggg ccgcgaggag gttggtgcgg cagagcagtt c
//
LOCUS       DAOQNN010000391         3049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_22974_length_3049_cov_14.482298, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000391 DAOQNN010000000
VERSION     DAOQNN010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22974_length_3049_cov_14.482298"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PKV97_15045"
     CDS             <1..1049
                     /locus_tag="PKV97_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB07248.1"
                     /translation="AGDGHDHGDEGSAPVGGNGPRRLPDGSVFLPKPAQRQIGVRTLV
                     TEAGELPRTVALAGKVVMDPNAGGKVQALVAGRLEAGPRGLPGVGQTVKKGEVLAYVV
                     PSAGQIERSNQMAQLAELQAAKVLAEKRVARLKELADTVPRKEIEAAESELASLAARA
                     TAVGAGLSNRDALVAPVAGVIASSNAVAGQVVDARELVFEIVDPKRLRVEALAYDADT
                     AIDVAGASLAVGEQRVRLDFLGAARSLREQALPLAFGAAGDSLARFAVGQPVEVFVQT
                     RSTLQGVSVPAGSVLKNPANQTIVWVKTAPERFEPRTVTIAPLDGANVAVTSGLAAGE
                     RVATNAATLINQIR"
     gene            1065..>3049
                     /locus_tag="PKV97_15050"
     CDS             1065..>3049
                     /locus_tag="PKV97_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB07249.1"
                     /translation="MFKWLLDNSLGNRLLVIIASLVLMAYGAFTLTRTPVDVFPDLNK
                     PTVTIMTESGGMAAEEVEQLITFPLETTMNGLPGVESVRSISSAGLSFVYVTFEWSTE
                     IFRARQMVSERLSAMEEGLPADVIPRMGPISSVMGEIMQIAIPIDTAKISPMQVREYA
                     DWVLRPRLMAIPGIAQVIPIGGEVRQFQVQPDTRRMAELGISLEQMEAAIRGFSSNTS
                     GGFLELTGREYLIRNLGRTSSLEDLKNLAITARDGQPILLRQIASVTFAPALRRGDAG
                     FEGKPAVILGIQKQPTADTIHLTRSIEAALDEMKKSLPAGMEAPQVTFRQASFIEASI
                     GTLQGKLIGASVFVAAILFFFLGTLRPTVIALTAIPVSIFMTALVFKYFGLSINTMTL
                     GGLAIAIGGLVDDAVVGVENVLRRLKEDRAKHHDHRLHPIELVAHATMEVRSAILYAT
                     IIIVLVFLPLFALPGMEGRLFVPLGVAFIVSTLASLVVSVTVTPVLSFYLLPRMKSLD
                     HGDTRVLAWLKARYRNGLQAVLERPRAAVFAGTVAVIVAAAAVPFFPTTFLPPFNEGT
                     LLIGMRLNPGVTLAESTALAQQAEVLVKQVPEVTHVGRRSGRAELDEHAEGVHVSELD
                     VGLKPASELTRSMGEIAADIRARLVNLPAAIAIGQPIS"
BASE COUNT          490 a          989 c         1053 g          517 t
ORIGIN      
        1 tcgctggcga cgggcacgat cacggcgacg aaggctccgc gcccgtcggc ggcaacggtc
       61 cgcgacgctt gcccgacggc agcgtattcc tgcccaagcc ggcacaacgg cagatcggcg
      121 tgcgcacgct ggtcacggaa gcgggggagc tgccccgcac cgtggcgctc gccggcaaag
      181 tggtgatgga ccccaacgcg ggcggaaaag tgcaggcgct ggtcgccggc cgcctcgaag
      241 ccgggccgcg cggcttgccc ggagtcgggc agaccgtgaa gaagggcgag gtgctggcgt
      301 atgtcgtgcc gtcggcgggg cagatcgaac gctcgaacca gatggcgcag ctcgccgagt
      361 tgcaggccgc caaagtgctc gcggagaagc gcgtggcgcg gctgaaggag ctcgccgaca
      421 cggtgccgcg caaggagatc gaagcggccg agagcgaact cgcgagtctc gcggcccgcg
      481 cgacggcggt gggtgcgggg ctcagcaatc gcgatgcgct cgtggcgccc gtcgcgggcg
      541 tcatcgcgtc gagcaacgcc gtcgccggtc aggtcgtcga tgcgcgcgaa ctggtgtttg
      601 aaatcgtcga tccgaagcgc ctgcgcgtcg aggcgctggc ctacgatgcc gacaccgcca
      661 tcgatgtggc cggcgcgagc ctcgcggtcg gcgagcaacg cgtgaggctc gacttcctcg
      721 gcgcggcgcg cagtctgcgc gagcaggcgt tgccgctcgc tttcggtgcc gcgggcgatt
      781 ccctggcgcg tttcgctgtc gggcaaccgg tcgaggtgtt cgtgcagact cgcagcacgc
      841 tgcagggcgt gagcgtccct gcaggatcgg tgctgaagaa cccggcgaac cagaccatcg
      901 tctgggtcaa gaccgcgccg gaacgcttcg agccgcgcac cgtcaccatc gccccgctcg
      961 atggggcgaa cgtcgccgtg acgtcgggcc tcgcggccgg ggagcgtgtc gccacgaacg
     1021 cggcaaccct gatcaaccag atccgctgac ggggagcctg accgatgttc aagtggctac
     1081 tcgacaacag tctgggcaac cggctgctgg tgatcatcgc cagcctggtg ctgatggcct
     1141 acggcgcgtt cacgctcaca cgcacgcccg tggacgtgtt cccggacctc aacaagccga
     1201 cggtcacgat catgaccgaa tccggcggca tggcggcgga ggaggtcgaa cagctcatca
     1261 ccttcccgct cgaaacgaca atgaacgggc tacccggggt ggagtcggtg cgctccatct
     1321 ccagcgcggg gctgtccttc gtctatgtga ccttcgagtg gagcacggag attttccgcg
     1381 cccggcagat ggtgtcggag cgcctgtcgg cgatggagga aggcctgccg gcggacgtga
     1441 ttccgcgcat ggggccgatt agctccgtga tgggcgagat catgcagatc gcgatcccga
     1501 tcgataccgc taagatctcg ccgatgcagg tgcgcgaata cgctgactgg gtgctgcgtc
     1561 cgcgtctcat ggccatcccc ggtatcgcgc aggtcatccc gatcggcgga gaagtgcggc
     1621 agttccaggt gcagccagat acgcggcgca tggcggaact cggcatttcg ctggaacaga
     1681 tggaagcggc gatccgcggc ttttcgtcga acacctcggg cggcttcctc gaactcaccg
     1741 ggcgtgaata cctgatccgc aacctcggac gcacctcgag cctggaggat ctgaagaatc
     1801 tcgcgatcac ggcacgcgac ggtcagccga tcctgctgcg ccagattgcc tccgtgacgt
     1861 tcgcaccggc gctgcgccgc ggcgatgcgg gtttcgaggg caaaccggca gtcattctcg
     1921 gtatccagaa gcagccgacc gcggacacca ttcacctcac ccgctcgatc gaggccgcgc
     1981 tcgacgagat gaagaaatcg ctgcccgccg gcatggaagc gccgcaggtt acgttccggc
     2041 aggcgagctt catcgaggca tcgatcggca cgctgcaggg caagctgatt ggcgcctcgg
     2101 tgttcgtcgc ggcgatcctg ttcttcttcc tcggcacgct gcgcccgacg gtgatcgcgc
     2161 tcactgcgat cccggtgtcg atcttcatga cggcgctggt gttcaagtac ttcgggttgt
     2221 cgatcaacac gatgacgctc ggggggctgg cgattgcgat cggcggtctg gtcgacgacg
     2281 cggtggtggg cgtcgagaac gtgttgcgcc ggctgaagga ggatcgcgcg aagcatcatg
     2341 accatcgcct gcacccgatc gaactcgtgg ctcatgcaac gatggaggtg cgctcggcca
     2401 tcctctacgc gacgatcatc atcgtgctgg tgttcctgcc gttgttcgcg ctgcccggga
     2461 tggaggggcg gctgttcgtg ccgctcggcg tggcattcat cgtctcgacg ttggcgtcgc
     2521 tggtcgtttc ggtaacggtg accccggtcc tgtccttcta tctgctgccg cgcatgaagt
     2581 ccctcgacca tggcgacacg cgcgtacttg cctggctcaa ggcgcgctat cgcaacggtc
     2641 tgcaggccgt cctcgagcgg cccagggcgg ccgtgttcgc cggcacagtc gcggtgatcg
     2701 tcgcggccgc cgccgtgccc ttctttccga ccacgtttct gccgcccttc aacgaaggca
     2761 ccctgctgat cggcatgcgg ctcaacccgg gggtgacgct cgccgagtcc accgcgcttg
     2821 cgcaacaggc ggaggtgctg gtgaaacagg tgccggaggt cacgcacgtt ggtcgtcgca
     2881 gcggacgtgc ggaactcgat gaacatgctg aaggcgtgca cgtcagcgag ctcgatgtcg
     2941 ggctcaagcc ggcgtcagaa ctcacccgtt cgatggggga gatcgccgcc gacatccgcg
     3001 cccggctcgt gaacctgccc gctgcgatcg cgatcggcca gccgatctc
//
LOCUS       DAOQNN010000392         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_23152_length_3035_cov_8.155705, whole genome shotgun sequence.
ACCESSION   DAOQNN010000392 DAOQNN010000000
VERSION     DAOQNN010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23152_length_3035_cov_8.155705"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PKV97_15055"
     CDS             <1..904
                     /locus_tag="PKV97_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNB07250.1"
                     /translation="VREAGGAQAGADGDSAGDEAVDDPPQRLPALDVVAERFDINELE
                     LGRLEVLARNRGNLWQLDRFALVSADGRLAGKGQWRAGARQRTELDFALETASMGGLI
                     RRLGHPDVVRGGAASLAGQLAWRGAPTRIDYPSLSGRLRLETGAGQFNRLEPGVGRLL
                     GILSLQSLPRRITLDFRDVFSEGFAFDRISGSIELARGVLRSEDLEIRGPAARVAMSG
                     SADVVSETQDLRVLVQPTLSESVAIGAAAGLVNPVAGVVTYLAQKVLSDPIEKLFAFE
                     YTVTGAWSDPQVAKRGRAAAPESR"
     gene            999..1823
                     /locus_tag="PKV97_15060"
     CDS             999..1823
                     /locus_tag="PKV97_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNB07251.1"
                     /translation="MTETSLSAPVRIAAIQTVSGPDVAANLETASHLIAQAAAAGARV
                     IALPEYFPLISKDETAKVRIREPEGAGPLQDFLAGEARRHGVWLIGGTLPLVAEADDK
                     VRNSTLVFDDQGRRVARYDKIHLFGFQRGEERYDEAATIEPGGTVACFDSPAGRIGLS
                     VCYDLRFPELFRAMGEVDLIVLPAAFTWTTGRAHWEVLLRARAIENQCYVLAPAQGGC
                     HESGRVTWGHTMIVDPWGEILACRDEGPGVVVADLDPARLAAVRGSLPALRHRCLA"
     gene            1944..>3035
                     /locus_tag="PKV97_15065"
     CDS             1944..>3035
                     /locus_tag="PKV97_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNB07252.1"
                     /translation="MSKSQNPLKVADRYLLSPYGLGEAELEKTFRKLMRHDIDFADLY
                     FQYQRSEAWSLEEGIVKSGSFDIEQGVGVRAISGEKTAFAYSDDISLDALVGAATATR
                     AIAAAGERRRVGIVPHKLRTRLYRADDPLDSLDDTAKVELLERLEGFARGEDPRVTQV
                     MAHIAGSWEVVMVARSDGHMAADVRPLVRVSITVIMEEDGRREQGSAGGGGRYDYGWF
                     CDDRLHDYARQAVHQARVNLGADPAPAGTMPVVLGPGWPGILLHEAIGHGLEGDFNRK
                     GSSAFSGRIGQQVAARGVTVVDDGTLPDRRGSLSIDDEGNPTERTVLIEDGILTGYMQ
                     DMMNARLMGMPVTGNGRRESFAHLPLPRMT"
BASE COUNT          423 a         1123 c         1065 g          424 t
ORIGIN      
        1 ggtgcgcgag gccggcggcg cgcaggctgg cgcagacggc gactccgcgg gcgacgaggc
       61 ggtggacgat ccgccgcagc gcctgcccgc gctcgacgtg gtggccgagc gcttcgacat
      121 caacgagctc gaactcggcc gcctcgaggt cctcgcacgc aaccgcggca acctctggca
      181 gctcgaccgc ttcgcgctgg tgagcgccga cggacgcctc gccggcaagg ggcagtggcg
      241 cgcgggcgcg cgccagcgca ccgagctcga cttcgcgctc gagaccgcgt cgatgggtgg
      301 gctgatccgg cgcctgggcc accccgacgt ggtgcgcggc ggcgccgcca gcctggccgg
      361 ccagctcgca tggcggggtg cgccgacccg gatcgactat cccagcctgt cgggcaggct
      421 gcggctggag accggcgcgg gccagttcaa ccggctcgaa cccggcgtcg gccgtctgct
      481 cggcatcctc agcctgcagt cgctgccgcg ccggatcacg ctcgacttcc gcgacgtgtt
      541 ctccgaagga ttcgccttcg atcgcatttc cggtagcatc gagcttgccc gtggtgtgct
      601 gcgcagcgag gacctggaga tccgcggacc cgcggcgcgc gtcgcgatga gcggcagcgc
      661 cgacgtggtc tccgaaacgc aggacctgcg cgttctggtg cagccgacgc tgtccgagtc
      721 ggtggcgatc ggcgcggcgg cgggcttggt gaatccggtc gccggagtgg tgacctatct
      781 cgcccagaag gtgctcagcg acccgatcga gaagctcttc gccttcgagt acacggtgac
      841 cggcgcgtgg tccgaccccc aggtcgccaa gcgcggcagg gccgctgcgc cggagtcgcg
      901 ctgatccggg ggcgagcgcc caagctgcgt gcccttcctt gcccaacccc cgcggggtct
      961 tgccgccagc ggcagcaccc cgccccaagc tcgccgacat gaccgaaacc tccctttccg
     1021 cgccggtgcg catcgccgcc atccagacgg tctccgggcc cgacgtcgcc gccaacctgg
     1081 aaaccgcgtc gcacctgatt gcccaggccg ccgccgccgg cgcccgcgtg atcgcgctgc
     1141 ccgaatactt tccgctcatc agcaaggacg agaccgccaa ggtgcgcatc cgcgagcccg
     1201 agggcgccgg cccgctgcag gatttcctcg ccggcgaggc gcgccgccac ggcgtgtggc
     1261 tgatcggcgg caccctcccg ctggtcgccg aggccgacga caaggtgcgc aacagcaccc
     1321 tggtcttcga cgaccagggc cggcgcgtgg cgcgctacga caagatccac ctgttcggct
     1381 tccagcgcgg cgaggagcgc tacgacgagg cggccaccat cgagccgggc ggcacggtcg
     1441 cctgcttcga ctcgccggcc gggcgcatcg ggctgtcggt gtgctacgac ctgcgcttcc
     1501 ccgagctctt ccgcgccatg ggcgaggtgg atctgatcgt gctgccggcc gccttcacct
     1561 ggaccaccgg ccgcgcgcac tgggaggtgc tgctgcgcgc gcgcgcgatc gagaaccagt
     1621 gctacgtgct ggcgccggcc cagggcgggt gccacgagag cgggcgggtg acctggggcc
     1681 acaccatgat cgtcgatccc tggggcgaga tcctcgcctg ccgcgacgag ggccccggcg
     1741 tggtggtcgc ggacctcgat cccgcgcgcc tcgcggcggt acgcgggagc ctgcctgccc
     1801 tgcgccaccg ctgcctggcc tgaacgcgcc gtccgcgagg gggcggacgt ggccgatgcc
     1861 gccgcctcct cgtgtcgtcg ccccgcaatg atcgtttttg cccgatgccg gtccaaccgg
     1921 catcctccgc caaggattta ccgatgagca agagccagaa tcccctcaag gtcgccgacc
     1981 gttacctgct gtcgccctac ggcctgggcg aggcggagct cgagaagacc ttccgcaagc
     2041 tgatgcgcca cgacatcgac ttcgccgacc tctacttcca gtaccagcgc tccgaggcct
     2101 ggagcctgga agagggcatc gtcaagtcgg gcagcttcga catcgagcag ggcgtgggcg
     2161 tgcgcgcgat cagcggcgag aagaccgcct tcgcctactc cgacgacatc tcgctcgacg
     2221 ccctggtcgg cgccgccacc gccacgcgtg cgatcgctgc ggccggcgag cgccgccgcg
     2281 tcggcatcgt cccgcacaag ctcaggaccc gcctgtaccg ggccgacgac ccgctcgact
     2341 cgctcgacga caccgccaag gtcgagctgc tcgagcgcct cgagggcttc gcccgtggcg
     2401 aggatccacg cgtcacccag gtcatggcgc acatcgccgg ctcgtgggag gtggtgatgg
     2461 tggcgcgcag cgacggccac atggcggccg acgtgcgtcc gctggtgcgc gtgtcgatca
     2521 ccgtgatcat ggaagaggac ggccgccgcg agcagggcag tgccggcggc ggcgggcgtt
     2581 acgactacgg ctggttctgc gacgaccgcc tgcacgacta cgcccgccag gcggtgcatc
     2641 aggcgcgcgt gaacctcggc gccgacccgg ccccggccgg caccatgccg gtcgtgctcg
     2701 gccccggctg gccgggcatc ctgctccacg aggcgatcgg ccacgggctg gagggcgact
     2761 tcaaccgcaa gggcagctcg gcgttttccg ggcgcatcgg ccagcaggtg gcggccaggg
     2821 gcgtcaccgt ggttgacgac ggcacgctgc ccgatcgccg cggctcgctg tcgatcgacg
     2881 acgagggcaa cccgaccgag cgcaccgtgc tgatcgagga cggcatcctc accggctaca
     2941 tgcaggacat gatgaatgcc cgcctgatgg gcatgccggt caccggcaac ggccgccgcg
     3001 agtccttcgc ccacctgccg ctgccgcgca tgacc
//
LOCUS       DAOQNN010000393         3032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_23211_length_3032_cov_11.984884, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000393 DAOQNN010000000
VERSION     DAOQNN010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23211_length_3032_cov_11.984884"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..783)
                     /locus_tag="PKV97_15070"
     CDS             complement(1..783)
                     /locus_tag="PKV97_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB07253.1"
                     /translation="MNTRIDLDIDQGIATVTLHNPTKLNAVNAAMWRGLSAAMARIAA
                     DDSVRCVIVRGAGENAFAAGGDIEEFRSVRATVDDALHYHEDLVASALNAIRDCAVPT
                     VAAIRGACVGGGLEIAGCCDIRIAGESARFGAPINRLGFSMYPGEMAGLLALVGPAVV
                     LEILLEGRILDAREALQKGLLTRVVDDERVFEEAAVSAERIRAGAPLVARWHKQWVRR
                     LTTGVALDEEEKRRAFDFLATADYREGLAAFSEKRAPVFRGR"
     gene            963..1271
                     /locus_tag="PKV97_15075"
     CDS             963..1271
                     /locus_tag="PKV97_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNB07254.1"
                     /translation="MYAHIRRNPRFAELVAKRTRFAGILAAIVLIVFYGFVLLVAFAP
                     ELIGKRLFEGSNLTFGILAGLLQFIFFWVLTLVYVRRANGEFDDINKELVKAAWKEEK
                     "
     gene            1271..2950
                     /locus_tag="PKV97_15080"
     CDS             1271..2950
                     /locus_tag="PKV97_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNB07255.1"
                     /translation="MNARSLSRSAAGLLALAASPLVLAAGPAMDAAEKQPLNLHAIGM
                     FFVFVLMTLGITYWAASRTKSTADFYTAGGGITGFQNGLAIAGDYMSAATLLGLTAMM
                     YAQGVDAYIYMLSFFVGWPIILFLMAERLRNLGKFTFADITAYRLDQGKVRTMAAVSS
                     LTVVCFYLVAQMVGAGQLIKLLFGLDYNIALVVVGALMMVYVTFGGMVATTWVQIIKA
                     CMLLIGGTAVGVLAFSQFGFSFDELTNRAMEVHKLGQKLLNPGSLLADPVTAISLGLG
                     LMFGTAGLPHILMRFFTVTDAKEARKSVLYASGIVAYFFNVIAIMGLCAIIIVGQNPQ
                     FFEGGVIGGKVIGGGNMIAMHLANAVGGNLLLGFLSAVAFATILAVVAGLALAGASAI
                     SHDIYSRVIMKGKADEATELRVSKFATIGLGVVAVLLGMAFEKMNIAFMVALAFGVAA
                     SANFPVLILSMYWKGLTTKGALVGGYSGLVSAVLFVLLSKSVWVDVLGNASAIFPYTQ
                     PALFSMPIAFFMTWLFSTLDKSKAAEAEKEAFEDQYVRAQTGVGAATASSH"
BASE COUNT          452 a         1053 c          958 g          569 t
ORIGIN      
        1 ctagcgcccc cggaacaccg gtgcccgctt ctcgctgaat gcggcaaggc cctcgcgata
       61 gtccgcggtg gcgaggaagt cgaaggcgcg gcgcttctcc tcctcgtcca gcgccacgcc
      121 ggtggtcagc cggcgcaccc attgcttgtg ccagcgcgcc accagcggcg cgcccgcgcg
      181 gatgcgctcg gccgacacgg cggcctcctc gaatacgcgt tcgtcgtcca ccacgcgggt
      241 cagcaggccc ttctgcagcg cctcgcgcgc atccaggatg cggccctcga gcaggatttc
      301 caggaccacc gccgggccga ccagggcgag caggccggcc atctcgcccg gatacatcga
      361 gaaccccagg cggttgatcg gcgcgccgaa gcgtgccgac tcgcccgcga tgcggatgtc
      421 gcagcagccg gcgatctcca gcccgccgcc cacgcaggcg ccgcggatcg ccgccacggt
      481 gggcacggcg cagtcgcgga tcgcgttcag cgcgctcgcc accaggtcct cgtggtagtg
      541 cagcgcatcg tccaccgtcg cgcgcacgct gcggaactcc tcgatgtcgc cgccggcggc
      601 gaaggcgttc tcgcccgcgc cgcgcacgat cacgcagcgc accgaatcgt cggcggcgat
      661 gcgcgccatc gccgccgaga gtccgcgcca catcgccgcg ttcacagcgt tcagcttggt
      721 cgggttgtgc agggtcacgg tggcgatgcc ctggtcgatg tcgaggtcga ttcttgtgtt
      781 catcggttcg ccgttttcga taaataaaat ttttccatac ggatgtgtat ggagtcttat
      841 ggatgatata tcatccgctc gcctacgatg ccgcgtcaga cggccgccga ggcaggagga
      901 aaggtggagg caaagcagtt cgtgttgctt atccttggga gggagtgaaa gtgtctagct
      961 cgatgtatgc gcacatccgg cgcaacccgc ggttcgcgga gcttgtcgcc aagcgcaccc
     1021 gcttcgcggg cattctggcg gcgatcgtcc tgatcgtctt ttacggcttc gtgctgctcg
     1081 tggcgttcgc gcccgagctc atcggcaagc gtctgttcga gggcagcaac ctgaccttcg
     1141 gcatcctcgc cggcctgctg cagttcatct tcttctgggt cctgaccctg gtgtacgtcc
     1201 gccgcgccaa cggcgagttc gacgacatca acaaggaact cgtgaaggcc gcctggaagg
     1261 aggaaaaatg atgaacgccc gtagcctttc ccgtagcgcc gccggcctgc tcgcgctggc
     1321 cgccagcccg ctggtgctcg ccgccggtcc cgcgatggac gccgccgaga agcagccgct
     1381 caacctccac gccatcggca tgttcttcgt gttcgtgctg atgaccctgg gcatcaccta
     1441 ctgggcggcc agccgcacca agtcgaccgc cgacttctac accgccggcg gtggcatcac
     1501 cggcttccag aacggcctcg cgatcgccgg cgactacatg tccgccgcca cgctgctcgg
     1561 tctcaccgcg atgatgtacg cgcagggtgt ggatgcctac atctacatgc tctccttctt
     1621 cgtgggctgg ccgatcatcc tgttcctgat ggccgagcgc ctgcgcaacc tgggcaagtt
     1681 caccttcgcc gacatcactg cctaccgcct cgaccagggc aaggtgcgca cgatggccgc
     1741 ggtgagctcg ctgaccgtgg tgtgcttcta cctggtggcg cagatggtcg gtgccggcca
     1801 gctcatcaag ctgctgttcg gtctcgacta caacatcgcg ctcgtcgtcg tcggcgcgct
     1861 gatgatggtg tacgtcacct tcggcggcat ggtcgccacc acctgggtgc agatcatcaa
     1921 ggcctgcatg ctgctgatcg gcggtaccgc ggtgggcgtg ctcgcgttca gccagttcgg
     1981 cttctcgttc gacgagctca ccaaccgtgc gatggaagtg cacaagctgg gccagaagct
     2041 gctcaatccc ggcagcctgc tcgccgatcc ggtgaccgcg atctcgctcg gcctcggcct
     2101 gatgttcggc accgccggcc tgccgcacat cctgatgcgc ttcttcaccg tgaccgatgc
     2161 caaggaagcg cgcaagtccg tgctctacgc ctcgggtatc gtcgcctact tcttcaacgt
     2221 catcgccatc atgggcctgt gcgcgatcat catcgtcggc cagaacccgc agttcttcga
     2281 aggcggcgtg atcggcggca aggtcatcgg cggcggcaac atgatcgcca tgcacctggc
     2341 caacgcggtc ggcggcaacc ttctgctcgg cttcctgtcg gcggtggcct tcgcgaccat
     2401 cctcgcggtg gtggccggcc tggcgctggc cggtgcctcg gcgatctcgc acgacatcta
     2461 ctcgcgcgtc atcatgaagg gcaaggccga cgaagcgacc gaactgcggg tgtcgaagtt
     2521 cgccaccatc ggcctcggtg tggtcgcggt gctgctcggc atggccttcg agaagatgaa
     2581 catcgcgttc atggtcgcgc tcgccttcgg cgtggccgct tcggccaact tcccggtgct
     2641 gatcctgtcg atgtactgga agggcctcac caccaagggc gccctcgtgg gcggctactc
     2701 cggcctggtc agcgcagtgc tgttcgtgct gctgtccaag tcggtgtggg tggatgtgct
     2761 gggcaacgct tcggcgatct tcccctatac ccagccggcg ctgttctcga tgccgatcgc
     2821 cttcttcatg acctggctgt tctccacgct cgacaagagc aaggcggccg aggccgagaa
     2881 ggaagccttc gaggaccagt acgtgcgcgc ccagaccggt gtcggcgccg cgaccgcctc
     2941 gtcgcactga ccgaagcgcg cggttccccg aaccgcgccg catccccgac ccgccccgcg
     3001 ctcccgcgcc gggcgggttt ttcgtcgacc gg
//
LOCUS       DAOQNN010000394         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_23943_length_2977_cov_12.032170, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000394 DAOQNN010000000
VERSION     DAOQNN010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23943_length_2977_cov_12.032170"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PKV97_15085"
     CDS             <1..942
                     /locus_tag="PKV97_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNB07256.1"
                     /translation="VKVGGKVHASPSVRAFARELGVDLGQVKASGPKNRILKEDVAAF
                     IKGAMSTGVVPGKTVAAAAVGSLGGGLDLLPWPKVDFAKFGEVEVKPLSRIKKISGQN
                     LARNWVMIPAVTYHEDADITDLEAFRVQMNKEYEKSGKKLTMLAFIIKASVRALQEFP
                     EFNTCLDGDNLVYKKYFNIAFAADTPNGLVVPVVKDADKKSVFDIAAETGALAKKARD
                     GKLGPADMSGACFTISSLGGIGGTYFAPIVNAPEVAILGVNKSVMKPVWDGKAFVPRL
                     TLPMSLTADHRVIDGALATRFNVYLAQLLADFRRVML"
     gene            1034..2851
                     /gene="lpdA"
                     /locus_tag="PKV97_15090"
     CDS             1034..2851
                     /gene="lpdA"
                     /locus_tag="PKV97_15090"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNB07257.1"
                     /translation="MSQMIEVKVPDIGDFDAVPVIELFVKVGDSIAVDDAICTLESDK
                     ATMDVPSSAAGVVREVLVNLGDKVGEGAVLIRLEAAGAAAGAAQAANTAPASTPASAP
                     APSAVAAVPAATPSAAPAAASHAGGADAEYDMVVLGAGPGGYSAAFRAADLGLRTAIV
                     ERYSTLGGVCLNVGCIPSKALLHVAAVIEEAEHVDKAGIVFAKPSVDVDALRKHKDGV
                     IGKLTGGLSGMAKARKVDILRGYGSFLDPNHLEIEETTGASQDKTGAKKVVKFRNCII
                     AAGSAAVHLPFLPRDPRIVDSTGALELRQVPGKMLVIGGGIIGLEMATVYSTLGARID
                     VVEMLDRLMQGPDADAVKVWEKQNAHRFDNIMLKTKTVAVEAKEDGLWVKFEGDKAPA
                     EPVRYDMILQSAGRSPNGRKIGAEKAGVIVGERGFIPVDAQMRTNVPHIFAIGDIVGQ
                     PMLAHKAVHEGHVAAEVAAGHKAAFDATVIPGVAYTHPEVAWVGYTEAQAKAEGKKVD
                     TAKFPWAASGRAIANGADYGFTKLIFDAETHRVIGGTIVGPSAGDMIGEVCLAIEMGA
                     DATDIGKTIHPHPTLGETVGMAAEVAHGSCTDLPPMRKK"
BASE COUNT          540 a          994 c         1014 g          429 t
ORIGIN      
        1 gtcaaggtcg gcggcaaggt gcacgccagc ccctcggtgc gcgcctttgc gcgcgagctc
       61 ggagtcgatc tgggccaggt caaggccagc gggccgaaga accgcatcct caaggaagac
      121 gtcgccgcct tcatcaaggg cgcgatgagc accggcgtgg tgccgggcaa gaccgtggcc
      181 gccgcggccg tgggcagcct gggcgggggg ctggatctgt tgccgtggcc caaggtcgac
      241 tttgccaagt tcggcgaggt cgaggtcaag ccgctctcgc gcatcaagaa gatctccggc
      301 cagaacctcg cccgcaactg ggtgatgatc ccggcggtga cctaccacga ggacgccgac
      361 atcaccgacc tggaagcctt ccgggtgcag atgaacaagg agtacgagaa gtcgggcaag
      421 aagctcacca tgctcgcctt catcatcaag gcctcggtgc gcgcgctgca ggagttcccc
      481 gagttcaaca cctgcctcga cggcgacaac ctggtctaca agaagtactt caacatcgcc
      541 ttcgcggccg acacccccaa cggcctggtc gtgccggtgg tgaaggatgc cgacaagaag
      601 agcgtgttcg acatcgccgc cgagaccggc gcgctcgcca agaaggcgcg tgacggcaag
      661 ctcggcccgg ccgacatgtc gggcgcgtgc ttcacgatca gctcgctcgg cggcatcggc
      721 ggcacctact tcgcacccat cgtcaatgcg cccgaagtgg ccatcctcgg ggtgaacaag
      781 tcggtgatga agccggtgtg ggacggcaag gctttcgtgc cgcgcctgac gctgccgatg
      841 tcgctgaccg cggaccaccg cgtcatcgac ggcgcgctgg ccacccgctt caacgtgtac
      901 ctcgcgcaac tgctggcgga cttccgccgg gtgatgctct gacacggtga ggagtgaggc
      961 gtgagcggtc aggggtgaaa agccccggcc gcggcgcctt cctcttccgg aaacgcctca
     1021 cgaggtgatg aagatgagtc aaatgattga agtaaaggtc cccgacatcg gcgatttcga
     1081 tgcggtgccg gtgatcgagc tgttcgtgaa ggtcggcgac agcatcgcgg tggacgatgc
     1141 gatctgcacg ctggaatccg acaaggccac gatggacgtg ccctcctccg ccgcgggcgt
     1201 ggtcagggaa gtgctggtca acctgggcga caaggtcggc gagggcgcgg tgctgatccg
     1261 gctcgaagcc gccggtgccg ctgcaggagc ggcgcaagcc gcgaacacgg cgcctgcttc
     1321 cacgcctgcc tccgcacctg caccctccgc agttgccgcc gttcctgcag ccacgccgag
     1381 cgccgccccc gcggcggcga gccacgccgg cggcgccgat gccgaatacg acatggtcgt
     1441 gctcggcgcc ggccccggcg gctactcggc ggccttccgc gccgccgacc tcgggctcag
     1501 gaccgcgatc gtcgagcgct attcgacgct cggcggcgtg tgcctgaacg tgggctgcat
     1561 cccctccaag gcgctgctgc atgtggccgc ggtgatcgag gaggccgagc acgtcgacaa
     1621 ggcgggcatc gtcttcgcca agccgagcgt ggacgtcgat gcgctcagga agcacaagga
     1681 cggcgtgatc ggcaaactga ccggggggct ctccggcatg gccaaggcgc gcaaggtcga
     1741 catcctgcgc ggctacggca gcttcctcga ccccaaccac ctcgagatcg aggagaccac
     1801 cggcgcgagc caggacaaga ccggcgcgaa gaaggtggtg aagttccgca actgcatcat
     1861 cgccgccggc tcggccgcgg tgcacctgcc cttcctgccg cgcgacccgc gcatcgtcga
     1921 ctccaccggc gcgctcgagc tgcgccaggt gccgggcaag atgctggtga tcggcggcgg
     1981 catcatcggc ctggagatgg ccacggtgta ctcgaccctg ggtgcccgca tcgacgtggt
     2041 cgagatgctc gaccgcctga tgcagggtcc ggacgccgac gccgtgaagg tatgggagaa
     2101 gcagaacgcc caccgcttcg acaacatcat gctcaagacg aagaccgtcg ccgtcgaagc
     2161 gaaggaagac ggcctgtggg tgaagttcga gggcgacaag gcacccgccg agccggtgcg
     2221 ctacgacatg atcctgcaat cggcgggccg ttcgcccaac ggcaggaaga tcggcgccga
     2281 gaaggcgggc gtgatcgtcg gcgagcgcgg cttcatcccg gtggatgcgc agatgcgcac
     2341 caatgtgccg cacatcttcg cgatcggcga catcgtcggc cagcccatgc tcgcccacaa
     2401 ggcggtgcat gaggggcacg tcgccgcgga agtggcagcc ggccataagg cggccttcga
     2461 cgccaccgtg atcccgggcg tggcctacac ccatccggaa gtggcctggg tcggctacac
     2521 cgaggcgcag gccaaggccg agggcaagaa ggtcgatacc gccaagttcc cgtgggccgc
     2581 ctccggccgc gccatcgcca acggcgccga ctacggcttc accaagctga tcttcgacgc
     2641 cgagacccac cgcgtgatcg gcggcaccat cgtcgggccg tcggccggcg acatgatcgg
     2701 cgaggtgtgc ctggccatcg agatgggggc cgacgccacc gacatcggca agaccatcca
     2761 cccgcatccg accctgggcg agacggtggg catggccgcc gaagtggccc acggcagctg
     2821 caccgacctg ccaccgatgc gcaagaagta agcattcgcg ggcagcagaa aaaaccccgg
     2881 tgccgctgcg cgggaccggg gttttttatc gtctgaagaa tgactcagaa cttgtagttg
     2941 atgcccgcgt agatcgaacg gcccgcaccc gggaccg
//
LOCUS       DAOQNN010000395         2969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_24051_length_2969_cov_8.365134, whole genome shotgun sequence.
ACCESSION   DAOQNN010000395 DAOQNN010000000
VERSION     DAOQNN010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24051_length_2969_cov_8.365134"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PKV97_15095"
     CDS             <1..778
                     /locus_tag="PKV97_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB07258.1"
                     /translation="GACQVAVLAAGSHDAAPLEAQAGHGQAILSALGYGGEHLRVIEA
                     DADDWQALERALYDWAPAMTVAEPARFRLLAKKRETLDFALRHLVAHAPATTTPAGLP
                     AAIALNAGAPFGQVIASDACTLCMSCTGACPAGALRAASDAYRLEFVEKNCLQCGLCE
                     ASCPESAITLEPRLLPGEYGTEGARRARALREADLFHCTACGKAMGAAPLIESMIARL
                     SGHAMFAGEAERARLRMCADCRVIDMMKAEAAVKAWDLTE"
     gene            789..1436
                     /locus_tag="PKV97_15100"
     CDS             789..1436
                     /locus_tag="PKV97_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNB07259.1"
                     /translation="MSAAVNASISIQPQPTWSDEDRARAEHYALIARLFHAAPDAALL
                     AALAQAGRTLGGEGGELPRAWAALGAAAQTMPLQAVAEEFDALFVSVGKPEVMCNGSW
                     YLTGFLQEEPLAELRDDLAELGLGRRAGVTETEDHIAALAEVMRHLVLTGPDDAGLAR
                     QRRFFGRHLAPWYAQFAGALAAAPGADFYAKVGGLLAAFMGIEREAFDMLEAEAR"
     gene            1504..1716
                     /locus_tag="PKV97_15105"
     CDS             1504..1716
                     /locus_tag="PKV97_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNB07260.1"
                     /translation="MKDENTKLSLSRRNFLAAIGAGSAAGAATLAATAAQASPVTAKV
                     VEEARREVADQGVSEHMRNYYRTTRI"
     gene            1730..>2969
                     /locus_tag="PKV97_15110"
     CDS             1730..>2969
                     /locus_tag="PKV97_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNB07261.1"
                     /translation="MLTKKSATAATSARRLRGAAARQLGQTMDRRAFLKRSGLGVGAG
                     ALATQLPYNFIGAADAAAPAGVSRAEGKAEIRRTVCTHCSVGCAADAVVRNGVWVHQE
                     PVFDSPINLGSHCAKGAALREHGHGEYRLKYPMKLVDGKYQKISWEQALNEVGDRLLK
                     IREESGPDAVYFIGSSKHNNEQAYLLRKFVSFWGTNNTDHQARICHSTTVAGVANTWG
                     YGAMTNSYNDMQNAKAMLFIGSNAAEAHPVSLLHILHAKENGAKMIVVDPRFTRTAAK
                     AHQYIRIRSGTDVPFLFGLLYHIFQNGWEDKQYIDDRVFGMDKIREEVLAKWTPDKVE
                     EACGVPAEQMLLAARTMAENRPSTIVWCMGQTQHSIGNAMVRASCILQLALGNIGKSG
                     GGANIFRGHDNVQGATDVGPN"
BASE COUNT          467 a         1077 c         1023 g          402 t
ORIGIN      
        1 cggcgcctgc caggtggcgg tgctcgccgc cggcagccac gacgccgcgc cgctcgaggc
       61 ccaggcgggt catggccagg ccatcctctc cgccctgggt tacggcggcg aacacctgcg
      121 cgtgatcgag gccgacgccg acgactggca ggcgctcgag cgcgcactgt acgactgggc
      181 tccggcgatg accgtcgccg aacccgcgcg tttccggttg ctggcgaaga agcgcgagac
      241 gctggacttc gcgctccgcc atctggtcgc ccacgcgcct gccacgacca cgcctgcggg
      301 cctgccggcg gcgatcgcgc tcaacgcggg cgcacccttc ggccaggtga tcgcatccga
      361 cgcctgtacc ttgtgcatgt cctgtaccgg cgcctgtccg gcgggcgcgc tgcgcgccgc
      421 cagcgacgcc tatcggctcg agttcgtcga gaagaactgc ctgcagtgcg gcctgtgcga
      481 ggcgtcctgc ccggagtccg cgatcacgct cgagccgcgc ctgctgccgg gcgagtacgg
      541 caccgaaggt gcgcgccgcg cgcgcgccct gcgcgaagcc gacctcttcc actgcaccgc
      601 ctgcggcaag gccatgggcg ccgcgccgct gatcgaatcc atgatcgccc gcctgtccgg
      661 ccacgccatg ttcgccggcg aggccgagcg cgcacgcctg cgcatgtgcg ccgactgccg
      721 ggtgatcgac atgatgaagg ccgaagcggc cgtcaaggcc tgggacctca ctgaatgaac
      781 gtctccgaat gagcgcagcc gtgaacgcca gcatttccat ccagccccag ccgacctggt
      841 cggacgagga ccgcgcgcgc gccgagcact acgcgctgat cgcccgtctg ttccacgccg
      901 cgcccgacgc tgcgctgctc gcggcgctcg cgcaggcggg gcggacgctc ggcggcgagg
      961 gcggcgagct gccgcgtgcc tgggccgctt tgggcgcggc ggcgcagacg atgccgctgc
     1021 aggcggtggc cgaggagttc gacgccttgt tcgtcagcgt cggcaagccc gaggtgatgt
     1081 gcaacggctc ctggtacctg accggcttcc tgcaggaaga gccgctcgcc gagctgcgcg
     1141 acgacctcgc cgaactcggc ctgggccgcc gcgcgggcgt gaccgagacc gaggaccaca
     1201 tcgccgcact cgccgaggtc atgcgccacc tggtgctgac cgggccggac gacgcggggc
     1261 tggcgcgcca gcggcgcttc ttcggccgcc acctcgcgcc ctggtacgcg cagttcgccg
     1321 gcgcgcttgc ggcggcgccg ggggcggact tctacgcgaa ggtcggcggc ctgctggcgg
     1381 ccttcatggg catcgagcgc gaggcgttcg acatgctgga ggcggaagcg cgctgagcgc
     1441 ggccgccatg gacacgatgc ggggcgggcg ggcgcctgcg ccgcgaatga ggggagagcg
     1501 aggatgaagg acgaaaacac caagctgtcg ctgtcgcgcc gcaacttcct ggccgcgatc
     1561 ggtgccggca gtgccgctgg cgccgccacg ctggccgcga ccgccgccca ggcgtcgcct
     1621 gtcaccgcca aggtggtcga ggaggcgcgc cgcgaggtcg ccgaccaggg cgtgagcgag
     1681 cacatgcgca attactaccg cacgacgcgg atctgaggag gacgcgagga tgttgacgaa
     1741 gaaatccgcg accgccgcca ccagcgcgcg tcgcctgcgc ggcgccgccg cgcgccagct
     1801 cggccagacc atggaccgcc gcgccttcct gaagcgctcc ggcctcggcg tgggcgccgg
     1861 cgcgctcgcc acccagctgc cctacaactt catcggcgcc gccgacgccg cggcgcccgc
     1921 gggcgtgtcg cgcgccgagg gcaaggccga gatccgccgc accgtgtgca cccactgctc
     1981 ggtgggctgt gccgccgacg cggtggtgcg caacggcgtg tgggtgcatc aggagccggt
     2041 gttcgactcg ccgatcaacc tcgggtcgca ctgcgccaag ggcgccgcgc tgcgcgagca
     2101 tggccacggt gaataccgcc tgaagtatcc gatgaagctg gtcgacggca agtaccagaa
     2161 gatctcgtgg gagcaggcgc tcaacgaggt cggcgaccgc ctgctgaaga tccgcgagga
     2221 gtccggcccc gacgcggtgt atttcatcgg atcgtcgaaa cataacaacg agcaggccta
     2281 cctgctgcgc aagttcgtct ccttctgggg caccaacaac accgaccacc aggcgcgcat
     2341 ctgccactcc accaccgtgg cgggcgtggc gaacacctgg ggctacggcg cgatgacgaa
     2401 ctcctacaac gacatgcaga acgccaaggc gatgctgttc atcggctcga acgcggccga
     2461 ggcgcacccg gtgtccttgt tgcacatcct gcacgccaag gaaaacggcg ccaagatgat
     2521 cgtcgtcgat ccgcgcttca cccgcaccgc ggccaaggcc caccagtaca tccgcatccg
     2581 ctccggcacc gacgttccct tcctgttcgg cctgctgtac cacatcttcc agaacggctg
     2641 ggaagacaag cagtacatcg acgaccgcgt cttcggcatg gacaagatcc gcgaggaagt
     2701 gctcgccaag tggacgccgg acaaggtgga agaagcctgc ggtgtgcccg cggagcagat
     2761 gctgctcgcc gcccgcacca tggccgagaa ccgcccgagc accatcgtct ggtgcatggg
     2821 ccagacccag cactcgatcg gcaacgccat ggtgcgcgcc tcctgcatcc tgcagctcgc
     2881 gctcggcaac atcggcaagt cgggcggtgg cgccaacatc ttccgcggcc acgacaacgt
     2941 gcagggcgcg accgacgtcg gccccaacc
//
LOCUS       DAOQNN010000396         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_24517_length_2938_cov_12.204648, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000396 DAOQNN010000000
VERSION     DAOQNN010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24517_length_2938_cov_12.204648"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..>1257
                     /locus_tag="PKV97_15115"
     CDS             113..>1257
                     /locus_tag="PKV97_15115"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNB07262.1"
                     /translation="MPVNELLVILLLIAASAFFSMSEISLAAARKIKLRVMAEAGHLN
                     ARRVLALQDSPGHFFTVVQIGLNAVAILGGVVGEQALSPYVTELLRRAYAGPMLDTIA
                     FVVSFVFVTSLFVLFADLMPKRLAMVQPERIAVAVVQPMQVCMWLFAPLVWVFNGAAN
                     LIFRWFKLPSVRIEDITADDIMAMADAGAQAGALLRQEQHLISNVFELDSRIVPSAMT
                     SRENIVFLTLSESEESIRRKIAAHPHGKFPVCEDGIDSVIGYVDSKDILPRIVQGQDL
                     SLRTQPIVRKVLMLPDTLTLFEALERFRDAKEDFALILNEYALVVGLLSLQDVMNTVM
                     GDLVSPFQEELIVRRDDNSWLIDGATPIEDVMQALEIEVFEGFQNYET"
     assembly_gap    1258..1357
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1424..2095)
                     /locus_tag="PKV97_15120"
     CDS             complement(1424..2095)
                     /locus_tag="PKV97_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNB07263.1"
                     /translation="MNATRIAPIALYQEVAERLRQRIFSHELPPGTWVDEQALAEHYG
                     ISRTPLREALKVLASEGLVTLKPRRGCYVTEISERDLDEVFSVMSMLEGECARLSAAR
                     ATEDDLARLRAIHANLEKAAGKPDIDRFFEANQAFHLALQEIADNRWLLHVIEDLRKV
                     IKLSRHHSLFSEGRLEQSLAEHRDILQALLDRDGARAETLMRTHILSGRAALARIAEA
                     KTQAA"
     gene            2506..>2938
                     /locus_tag="PKV97_15125"
     CDS             2506..>2938
                     /locus_tag="PKV97_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNB07264.1"
                     /translation="MSKANDPVYPQPDIEAWKKAAAKQAPGGDLDKLNWITPEGLSVK
                     PLYTKADTEGLQHADTLPGFEPFLRGPQPTMYAVKPWTIRQYAGFSTAEASNAFYRKA
                     LAAGGQGVSVAFDLATHRGYDSDHPRVTGDVGKAGVAIDSVE"
BASE COUNT          440 a          960 c          941 g          497 t
ORIGIN      
        1 ggggggcggc gatgcgccgg ggcaccgatt gcccttcagg ccgcggtggt agactcgcag
       61 actcgattct ggttttgcct tccggcggga ccttcatccc aaaggagtcg tcgtgcctgt
      121 caacgaactg ctcgtcatcc tgctgctgat cgcagcgagc gccttctttt ccatgtccga
      181 gatctccctc gcggctgcgc gcaagatcaa gctgcgcgtc atggccgagg cagggcatct
      241 caacgcccgg cgcgtgcttg cgctgcagga cagtccgggc cacttcttca ccgtggtgca
      301 gatcggcctc aacgccgtcg ccattctcgg cggcgtagtc ggcgaacagg cgctttctcc
      361 ctacgtcacc gagctgctgc gccgggccta cgccggcccg atgctcgaca ccatcgcctt
      421 cgtcgtctcc ttcgttttcg tcacctcgct gttcgtgctt tttgccgacc tcatgcccaa
      481 gcgcctggcc atggtccagc ccgaacgcat cgcggtggcg gtggtgcagc cgatgcaggt
      541 gtgcatgtgg ctgttcgcgc cgctggtgtg ggtcttcaac ggcgcggcca acctcatctt
      601 ccgctggttc aagttgccga gcgtgcgcat cgaggacatc accgccgacg acatcatggc
      661 catggccgac gccggtgccc aggccggcgc gctgctgcgc caggagcagc acctgatcag
      721 caacgtgttc gagctcgact cgcgcatcgt gccctcggcg atgacctcgc gcgagaacat
      781 cgtcttcctc accctgtcgg agtccgagga gagcatccgg cgcaagatcg ccgcccaccc
      841 gcacgggaag tttccggtgt gcgaggacgg catcgacagc gtgatcggct atgtggactc
      901 gaaggacatc ctgccgcgca tcgtgcaggg ccaggatctg tccttgcgca cccagccgat
      961 cgtgcgcaag gtgctgatgc tgcccgacac gctcacgctc ttcgaggcgc tcgagcgctt
     1021 ccgcgacgcc aaggaggatt tcgcgctcat cctcaacgaa tacgcgctgg tggtgggcct
     1081 gctgtcgctg caggacgtga tgaacacggt gatgggcgat ctcgtcagcc ccttccagga
     1141 agagctcatc gtgcgccgag acgacaactc ctggctcatc gacggcgcca cgccgatcga
     1201 ggacgtcatg caggcgctcg agatcgaggt cttcgagggc ttccagaact acgagacnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngac gcgagtcgcg attgcgggcg
     1381 tgtcggccgg gtcgctccca cacgaaatca atgtcgccag gcctcacgcg gcctgcgtct
     1441 tcgcctcggc gatgcgggcg agggcggcgc ggccgctgag gatgtgggtg cgcatcaggg
     1501 tctcggcgcg cgcgccgtcg cggtcgagca aggcctggag gatgtcgcgg tgctcggcga
     1561 gcgactgctc cagccggccc tccgagaaca gcgagtggtg gcgcgacagc ttgatcacct
     1621 tgcgcaggtc ctcgatcacg tggagcaacc agcgattgtc ggcgatctcc tgcagggcga
     1681 ggtggaaggc ctggttggcc tcgaagaagc ggtcgatgtc gggcttgccg gcggccttct
     1741 cgaggttggc gtggatcgcg cgcaggcggg cgaggtcgtc ctcggtcgcg cgcgcagccg
     1801 acagccgggc gcactcgcct tcgagcatcg acatcacgct gaacacctcg tcgagatcgc
     1861 gctccgagat ctcggtcacg tagcagccgc gccgcggctt gagcgtcacc aggccctcgg
     1921 aggccagtac cttcagcgcc tcgcgcagcg gtgtgcgcga gatgccgtag tgctcggcca
     1981 gcgcctgttc gtccacccag gtgccgggcg gcagctcgtg cgagaagatc cgctggcgca
     2041 ggcgctcggc cacttcctgg tagagcgcga tcggagcgat gcgggtggcg ttcatgggcg
     2101 gattctttct tcggagcgga ttcaaaaggt ggcgcggggc agggccatca cgggtggtgg
     2161 tggcgcgtgc atgccctctc tcgccggacg ggcccggctc gctgcgcgcc ctttccgacc
     2221 gtcggaacgc attcgcccgc tgccgcctat tgcattcata attatggata aagtattatt
     2281 tttcctgcag tgcaagtcaa cttccgaagc gcgatgcgtc atgggaggcg ccgggaccac
     2341 ccggcggaag agaggggacg cgtcgcttgc cggagcgtcg acggccgctc gtgggatcgc
     2401 cacgggcggc ttcgtcgccg ccccgggggg gtttggcttg ccgataaatc accaatccgg
     2461 gtcgcgcctg taccccaggc cgccgcagat ctcacgaggt ttcccatgtc gaaagccaac
     2521 gatcccgtct atccccagcc cgacatcgag gcctggaaaa aggctgccgc caagcaggcg
     2581 ccggggggcg acctcgacaa gctgaactgg atcacgcccg agggcctctc ggtcaagccg
     2641 ctgtacacca aggccgacac cgaaggcctg cagcacgccg acacgctgcc cggcttcgag
     2701 cccttcctgc gcggcccgca gcccaccatg tacgcggtca agccgtggac catccgccag
     2761 tacgccggct tctccaccgc cgaagcgtcc aacgccttct accgcaaggc gctcgccgcc
     2821 ggcggccagg gcgtgtcggt cgcgttcgac ctggcgaccc accgcggcta cgactccgac
     2881 catccgcgcg tgaccggcga cgtcggcaag gccggcgtgg cgatcgactc ggtcgagg
//
LOCUS       DAOQNN010000397         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_24583_length_2933_cov_9.990618, whole genome shotgun sequence.
ACCESSION   DAOQNN010000397 DAOQNN010000000
VERSION     DAOQNN010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24583_length_2933_cov_9.990618"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKV97_15130"
     CDS             <1..197
                     /locus_tag="PKV97_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07265.1"
                     /translation="DPNSPSGFRYVRRSEAGERPREAMRGFRRDDDGESRRSFRRDAR
                     EDGARPEPQGREPAGNRAAN"
     gene            338..1135
                     /locus_tag="PKV97_15135"
     CDS             338..1135
                     /locus_tag="PKV97_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB07266.1"
                     /translation="MKLSSLLMLSLTAGALLAGCGKNETAPAAQSAAAPAAPAKIVIG
                     LDDNFPPMGFRNEKNELVGFDIDLAKEAAKRLGIAVEFKPIDWSAKEAELNGKRVDAL
                     WNGLTITEERRKNIGFTSAYMENHQIIVVPAASAIKTKAELAGKVIGIQDGSSAVDAV
                     QKDPVAASFKELKKFGDNVTALMDLTTGRLDAVVLDEVVGRYYTAKKPNDYVVLDDHF
                     GTEEYGVGTRKDDTALLAKLQKAMDEMKADGSAARISTEWFGKNIIK"
     gene            1298..1948
                     /locus_tag="PKV97_15140"
     CDS             1298..1948
                     /locus_tag="PKV97_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNB07267.1"
                     /translation="MDYILSILGPLAEGSLVTLQLFLITLLLAVPLGLALALVRISRF
                     RVASQAVNAYIWLMRGTPLMLQMLFIYYALPFVPGIGIRLPDFPAAIVAFALNYAAYF
                     AEIFRAGIQSIDRGQYEGAKVLGFSYGQTMRRIVLPQVVKRILPPMSNETITLVKDTS
                     LIYVLALNDLLRAARGIVQRDFTITPFIVAAAFYLIMTLVLTWLFQRLEKRHAVYD"
     gene            1983..2735
                     /locus_tag="PKV97_15145"
     CDS             1983..2735
                     /locus_tag="PKV97_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNB07268.1"
                     /translation="MILADGIRKRFGANEVLKGVSLTLAKGEVVAVIGPSGSGKSTFL
                     RCLNHLETIDAGRIVIEGDVLADNDTGGRARYVADAEVRRICARMGMVFQHFNLFPHL
                     TVLENVIEAPITVKGLKREAILPKAEALLRKVGLFDKRDAYPSRLSGGQKQRVAIARA
                     LAMEPDIMLFDEPTSALDPELTGEVLRTMRQLADEHMTMLVVTHEMGFAREVASRVVF
                     MDGGELIESRPAAEFFANPQHPRTRAFLDSML"
BASE COUNT          495 a         1089 c          932 g          417 t
ORIGIN      
        1 cagaccccaa ctcgccgagc ggcttccgct acgtgcgccg cagcgaggcg ggcgagcggc
       61 cacgcgaggc gatgcgcggt tttcggcgtg acgacgacgg ggagagccga cgcagcttcc
      121 ggcgcgacgc gagggaagac ggcgcccgtc ccgagcctca gggacgcgag cccgcaggca
      181 accgggcagc caactgagcg ccccttacgg gcagcggcaa ggttgctcgc ttgacgcagt
      241 gcagcgagaa tcacggtttt ggcgccggca ccccggcgcc ctctccgtgg cgggcccacc
      301 cggcccgtcc ccgaccaccc tcccgggcaa gaccaagatg aaactctcct cgctgctgat
      361 gctctccctc accgccggtg cgctgctcgc cggctgcggc aagaacgaaa ccgcgccggc
      421 cgcccaatcc gccgcagccc ccgccgcccc ggcgaagatc gtcatcggcc tcgacgacaa
      481 cttcccgccc atgggcttcc gcaacgagaa gaacgaactg gtcggcttcg acatcgacct
      541 cgccaaggaa gcggccaagc gcctcggcat cgccgtcgag ttcaagccga tcgactggag
      601 cgccaaggaa gccgagctca acggcaagcg cgtcgatgcg ctgtggaacg gcctcaccat
      661 caccgaggag cgcaggaaga acatcggctt caccagcgcc tacatggaga accaccagat
      721 catcgtggtg cccgccgcct cggcgatcaa gaccaaggcc gagctcgccg gcaaggtgat
      781 cggcatccag gacggcagca gcgcggtcga cgcggtgcag aaggacccgg tggccgcctc
      841 cttcaaggag ctgaagaagt tcggcgacaa cgtcaccgcg ctgatggacc tcaccaccgg
      901 ccgcctcgac gcggtggtgc tcgacgaggt cgtgggccgc tactacaccg ccaagaagcc
      961 gaacgactac gtggtgctcg acgaccactt cggcaccgag gaatacggcg tcggcacccg
     1021 caaggacgac accgcgctgc tggccaagct gcagaaggcg atggacgaga tgaaggccga
     1081 cggcagcgcc gcccgcatct ccaccgagtg gttcggcaag aacatcatca agtaagccgg
     1141 ctgcagagat cgcggcttgc gccgctccta cgtcggcccc gtgtaggagc ggcgcaagcc
     1201 gcgaattcct ccgctccccc cactcgggcg ccaacgccgc ccgcgccgac gctcccgcgc
     1261 gcctcctacc tgcccgccgc tcgcccggcg tcccgccatg gactacatcc tcagcatcct
     1321 cggcccgctc gccgagggct cgctggtcac gctgcagctc ttcctgatca ccctgctgct
     1381 ggccgtgccg ctcggcctgg cgctggcgct ggtgcgcatc tcgcgcttcc gcgtcgccag
     1441 ccaggcggtc aacgcctaca tctggctgat gcgcggcacg ccgctgatgc tgcagatgct
     1501 gttcatctac tatgcgctgc ccttcgtgcc ggggatcggc atccgcctgc ccgacttccc
     1561 ggcggcgatc gtggccttcg cgctcaacta cgcggcctac ttcgccgaga tcttccgcgc
     1621 cggcatccag tcgatcgacc gcggccagta cgagggcgcc aaggtgctcg gcttctccta
     1681 cggccagacc atgcgccgca tcgtgctgcc gcaggtggtc aagcgcatcc tgccgccgat
     1741 gagcaacgag accatcaccc tggtcaagga cacctcgctg atctacgtgc tcgcgctcaa
     1801 cgacctgctg cgcgccgcgc gcggcatcgt gcagcgcgac ttcacgatca cccccttcat
     1861 cgtcgccgcc gccttctacc tgatcatgac gctggtgctg acctggctct tccagcgcct
     1921 ggagaaacgc catgccgtct atgactgagg ccgccgcgat gacggggccg atgacgagcc
     1981 cgatgatcct cgccgacggc atccgcaagc gcttcggcgc caacgaggtg ctcaagggcg
     2041 tgtcgctgac gctcgccaag ggcgaggtgg tggcggtgat cgggccttcg ggctcgggca
     2101 agagcacctt cctgcgctgc ctcaaccatc tggaaacgat cgacgccggg cgcatcgtca
     2161 tcgagggcga cgtcctcgcc gacaacgaca ccggcggccg cgcgcgctac gtcgccgacg
     2221 ccgaggtgcg ccgcatctgc gcgcgcatgg gcatggtgtt ccagcacttc aacctgttcc
     2281 cccacctcac cgtgctggag aacgtgatcg aggccccgat cacggtgaag ggcctgaagc
     2341 gcgaggccat cctgcccaag gccgaagcgc tgctgcgcaa ggtcggcctc ttcgacaagc
     2401 gcgacgccta cccctcgcgc ctgtccggcg gacagaagca gcgcgtggcg atcgcgcgcg
     2461 cgctggcgat ggagcccgac atcatgctct tcgacgagcc cacctcggcg ctcgaccccg
     2521 agctcaccgg cgaggtgctg cgcaccatgc gccagctcgc cgacgagcac atgaccatgc
     2581 tggtggtgac gcacgagatg ggcttcgccc gcgaggtggc cagccgggtg gtgttcatgg
     2641 acggcggcga gctcatcgag tcgcgcccgg cggcggagtt cttcgccaac ccgcagcatc
     2701 cgcgcacgcg cgccttcctc gacagcatgc tgtagcaggc gggcgtcggc cgacacggcg
     2761 cgggtggaac ggggaatcgc gactcgcgtc gctcccaccg aaaaccgggc gatgcgcttg
     2821 gggacacgac cgtaggagcg acgcaagtcg cgattgcagc ccgcttcccc cgccaacacc
     2881 cgtcgcagcc ggaccacccg atcgcggctt gcgccgctcc tacaggtccc gga
//
LOCUS       DAOQNN010000398         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_24701_length_2925_cov_7.622300, whole genome shotgun sequence.
ACCESSION   DAOQNN010000398 DAOQNN010000000
VERSION     DAOQNN010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24701_length_2925_cov_7.622300"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PKV97_15150"
     CDS             complement(<1..734)
                     /locus_tag="PKV97_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNB07269.1"
                     /translation="MAEINLVEAVNLALAHELARDEAVVLLGEDIGANGGVFRATVGL
                     QQRFGAGRVVDTPLAETAIVGTAIGMAAMGLRPVAEIQFSGFLYPAIDHLINHAARLR
                     NRTRGRLSCPLVVRTPCGGGIHAPEHHSESPEAMLAHTPGLRVVMPSSPARAYGLLLA
                     AIRDPDPVMFLEPTRMYRLFRQEVADDGEALPLDVCFTLRGGTDLTLVSWGAMLHETQ
                     AAADALATEGISAEVIDVATLKPLDLP"
     gene            complement(727..1830)
                     /gene="pdhA"
                     /locus_tag="PKV97_15155"
     CDS             complement(727..1830)
                     /gene="pdhA"
                     /locus_tag="PKV97_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNB07270.1"
                     /translation="MSTVARFQIDHVRFLDPQGEALGPLPDFARDPAQLVPMYRAMYL
                     TRTFDAKAIALQRTGKLGTFASALGQEAIGVGVASAMRAEDALFPSYREHAAQLVRGV
                     RIEESLRYWGGDERGSDFAQARHDFPNCVPIGTQVCHAAGAAYAFKLRGEARAAVCFL
                     GDGGSSKGDFYEGLNFAAVWKAPLLIVVSNNQWAISVPRSAQSATATLAQKAIAAGVD
                     GVQVDGNDLVAVHHVAREALAKARAGGRPTLIEAVTYRLGDHTTADDASRYRDPAVVQ
                     EQWSFEPLTRLRNHLVGLGAWGPDKEEALARECTETVNAAVEAYLNTPPPTTAAMFEH
                     LFATLPAALVAQRDEALRFAPRKAGGQPGGDHG"
     gene            complement(2020..2700)
                     /locus_tag="PKV97_15160"
     CDS             complement(2020..2700)
                     /locus_tag="PKV97_15160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNB07271.1"
                     /translation="MSAQASDWRVPAATLRWLDAVPAERGVAMLVRHSVRDPLPPGDL
                     GYANALNAEGVELARSLGERLHGRLRSLHTSPLQRCVHTAEVFARVAGDTPPIVTDRM
                     LGDPGAWVLDRALAGENWKRLGSEGIMEQLATTDEDLPGTRPARVGAQNLAMHMLAIA
                     AGSPGLHVFVTHDILLAIAAARFLGPPPAGPERWPGYLEAAFFWQDDDGLHAGYRDRH
                     RGGLTGAR"
     gene            complement(2717..>2925)
                     /locus_tag="PKV97_15165"
     CDS             complement(2717..>2925)
                     /locus_tag="PKV97_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNB07272.1"
                     /translation="VFRVERQRLLKDKHLKLELARGSTRYEAIRFNHAEGAAGQIHAA
                     FRLGINEYNGVASVQLMLEHFEAA"
BASE COUNT          374 a         1005 c         1128 g          418 t
ORIGIN      
        1 ggcaggtcga gcggcttgag cgtggcgacg tcgatgacct cggccgagat gccctcggtg
       61 gcgagcgcgt cggccgcggc ctgggtctcg tgcagcatcg cgccccagct caccagggtg
      121 aggtcggtgc cgccgcgcag cgtgaagcac acgtccagcg gcagcgcctc gccgtcgtcg
      181 gccacctcct ggcggaacag gcggtacatc cgcgtcggct cgaggaacat caccgggtcg
      241 gggtcgcgga tcgctgccag cagcaggccg taggcgcgtg cgggcgagga cggcatgacc
      301 acgcgcaggc cgggggtgtg ggcgagcatc gcctccgggc tctcggagtg gtgctcgggc
      361 gcatggatgc ctccgccgca gggcgtgcgc accaccagcg ggcagctgag gcggccgcgg
      421 gtgcggttgc gcaggcgcgc ggcatggttg atcaggtggt cgatcgccgg atacaggaag
      481 ccggagaact ggatctccgc caccggcctg aggcccatcg ccgccatgcc gatcgcggtg
      541 ccgacgatcg cggtctcggc gagcggggtg tcgaccacgc gcccggcgcc gaagcgctgc
      601 tgcagcccga ccgtggcgcg gaacacgccg ccgttggcgc cgatgtcctc gccgagcagc
      661 acgaccgcct cgtcgcgcgc gagctcgtgg gcgagggcca ggttgaccgc ctcgacgaga
      721 ttgatctcag ccatgatcgc ctcccggttg tccgcccgcc ttgcgcggcg cgaagcgcag
      781 ggcctcgtcg cgctgggcca cgagcgcggc gggcagggtc gcgaagaggt gctcgaacat
      841 cgccgcggtg gtgggcggcg gggtgttcag ataggcctcg accgcggcat tcacggtctc
      901 ggtgcattcg cgcgcgagcg cctcctcctt gtccggcccc caggcgccga gcccgaccag
      961 gtggttgcgc agccgggtca gcggctcgaa ggaccactgc tcctgcacca ccgcagggtc
     1021 gcggtagcgc gaggcgtcgt cggcggtggt gtggtcgccg aggcgatagg tgaccgcctc
     1081 gatcagggtc ggtcgaccgc cggcgcgggc cttcgccagc gcttcacggg caacgtggtg
     1141 cactgcaacg aggtcgttgc cgtcgacctg cacgccatcg acgcctgcgg cgatggcctt
     1201 ctgcgccagc gtggcggtgg cgctctgcgc gctgcgcggc accgagatcg cccactggtt
     1261 gttgctcacc acgatcagca ggggcgcctt ccagacggcg gcgaagttga gcccctcgta
     1321 gaagtcgccc ttcgagctgc cgccatcgcc gaggaagcac accgctgcgc gcgcctcgcc
     1381 gcgcagcttg aaggcatagg ccgcgcccgc ggcgtggcag acctgggtgc cgatcggcac
     1441 gcagttggga aagtcgtggc gggcctgggc gaagtcgctg ccgcgctcgt cgccgcccca
     1501 gtagcgcagg ctctcctcga tgcgcacgcc gcgcacgagc tgggcggcgt gctcgcgata
     1561 ggagggaaac agcgcgtcct cggcacgcat cgcgctcgcc accccgaccc cgatcgcctc
     1621 ctggccgagc gcagacgcga aggtcccgag cttgccggtg cgctgcagcg cgatcgcctt
     1681 ggcgtcgaag gtgcgcgtga ggtacatcgc gcggtacatc gggaccagct gtgcgggatc
     1741 gcgcgcgaag tcgggcagcg gcccgagcgc ctcgccctgc gggtcgagga aacggacgtg
     1801 atcgatctgg aaccgggcga cagtgctcat ggcgcgctcc cttcaggctc ggtcggcccc
     1861 gcatgcctgc gcgccccggt cggggtccgt ctcctctggg cgccggtggc atccgggcgt
     1921 ggatgtcgtc ggcgaccggg tggcggcgcc gcgacgtttt ccgtttgcgg ggttggagcg
     1981 cgcccgtgcg cggatgttcc cgacgcggtt gccgcttcgt cagcgcgcgc cggtgaggcc
     2041 gccgcggtgg cggtcgcgat agccggcgtg gaggccgtcg tcgtcctgcc agaagaaggc
     2101 ggcctcgagg tagcccggcc agcgctccgg gccggccggc ggcgggccga ggaagcgcgc
     2161 cgccgcgatc gcgagcagga tgtcgtgggt gacgaagacg tgcagaccgg gcgagcccgc
     2221 ggcgatcgcg agcatgtgca tggcgaggtt ctgcgcgccc acgcgcgccg gacgcgtgcc
     2281 gggcaggtcc tcgtcggtcg tcgccaactg ctccatgatc ccctcgctgc cgaggcgctt
     2341 ccagttctcg cccgccagcg cgcggtcgag cacccaggcg ccggggtcgc cgagcatgcg
     2401 gtcggtgacg atcggcggcg tgtcgccggc cacgcgcgcg aagacctcgg cggtgtgcac
     2461 acagcgctgc agcgggctgg tgtgcaggct gcgcaggcgt ccgtgcaggc gctcgccaag
     2521 gctgcgcgcg agctcgacgc cttcggcgtt gagcgcgttg gcgtagccga ggtcgccggg
     2581 cggcaagggg tcgcgcaccg aatggcggac cagcatggcc acgccgcgct cggccggcac
     2641 cgcgtccagc cagcgcaggg tggcggcggg gacccgccag tccgaggctt gcgcgctcat
     2701 cgcgatggcg gcgggcctac gccgcctcga aatgttcgag catcagctgc acgctggcga
     2761 cgccgttgta ttcattgatg ccgaggcgga aggcggcgtg gatctggccg gcggcgccct
     2821 cggcgtggtt gaagcggatg gcctcgtagc gcgtgctgcc gcgcgcgagc tcgagcttga
     2881 ggtgcttgtc cttcaacagg cgctggcgct cgacgcggaa gacgt
//
LOCUS       DAOQNN010000399         2921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_24768_length_2921_cov_9.241452, whole genome shotgun sequence.
ACCESSION   DAOQNN010000399 DAOQNN010000000
VERSION     DAOQNN010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24768_length_2921_cov_9.241452"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /gene="era"
                     /locus_tag="PKV97_15170"
     CDS             complement(<1..900)
                     /gene="era"
                     /locus_tag="PKV97_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNB07273.1"
                     /translation="MSDASQTPPSDQPTGEDGFRAGFVAIVGRPNVGKSTLLNRLIGQ
                     KISIVSSKAQTTRHRVTGILTEPGAQFVFVDTPGFQTHHRNALNRSMNRTVSQVLADV
                     DLVFFVIEAGRFTEDDRKVLAVIPESSKVILVINKVDQLKDKTRLLPFIAQVREARDF
                     TEIVPLSAEKGRNVDELVKAATPLLPVGTPMFEEDEITDRSERFLASEFLREKLFRLL
                     GDELPYGIAVEIEKFEVEGNLRRIYAAVIVDKPGHKSMVIGRGGEKLKRISSEARVEL
                     EKLFDGKVFLEVWVKVKSGWADDE"
     gene            complement(897..1568)
                     /gene="rnc"
                     /locus_tag="PKV97_15175"
     CDS             complement(897..1568)
                     /gene="rnc"
                     /locus_tag="PKV97_15175"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNB07274.1"
                     /translation="MMPDRLQARLGHAFSDVRLLQEALTHRSFGQPNNERFEFLGDAV
                     LDCVVSIALFGRFGELREGELSRVRASLVRQDTLHRLALELELGDCLRLGEGELKSGG
                     SRRPSILADALEALFAAVYLDAGFEAAKGVIDRLYAPLIAEVDPCKPSKDPKTALQEW
                     LQARRIPVPSYTMVEIVGEAHAQEFEVACEIPKLAVRTLGRGPSRRIAEQQSAELALA
                     AVRKK"
     gene            complement(1573..1935)
                     /locus_tag="PKV97_15180"
     CDS             complement(1573..1935)
                     /locus_tag="PKV97_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HNB07275.1"
                     /translation="MKMRSQRGLTLLGVLVTGVIAAFVLVVGFRSVPVINEYLAIKRI
                     LGIVADESDKGASLIEVRRSFDRRGQIDDVSSVGGADLTIDKSGVRTVIDVEYTRKIP
                     LLANVSLSIDLHPSSEPR"
     gene            complement(1949..2737)
                     /gene="lepB"
                     /locus_tag="PKV97_15185"
     CDS             complement(1949..2737)
                     /gene="lepB"
                     /locus_tag="PKV97_15185"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNB07276.1"
                     /translation="MNFALILFLLLIASGALWSVDRFHARKLRPAGTPDPWWVEYGAS
                     FFPVILIVFGLRSFVVEPFKIPSGSMIPTLLVGDFILVNKWTYGIRLPVINRKIVAVN
                     DPQRGDVMVFRYPADPSMDYIKRVVGLPGDRVEYIDKRLTINGEAVAIREDGSYLHPD
                     RLYYSPKYVEKLGEVEHSVLIERDAPAYVPQVLNFPGREHCTYVHAGVRCTVPAGHYF
                     VLGDNRDASSDSRVWGFVPDQNIVGRAFFIWFNFGDMKRIGGFD"
     gene            complement(2778..>2921)
                     /locus_tag="PKV97_15190"
     CDS             complement(2778..>2921)
                     /locus_tag="PKV97_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07277.1"
                     /translation="KCYGGDITRKKKLLEKQKEGKKRMKQVGNVEIPQEAFLAVLRTD
                     EGK"
BASE COUNT          482 a          946 c          985 g          508 t
ORIGIN      
        1 ctcgtcgtcg gcccagccgc tcttgacctt gacccagacc tcgaggaaga ccttgccgtc
       61 gaagagcttc tcgagttcga cgcgggcctc gctggagatg cgcttgagct tctcgccacc
      121 gcggccgatg accatggact tgtggccggg cttgtcgacg atcaccgcgg cgtagatgcg
      181 gcgcaggttg ccctcgacct cgaatttctc gatctcgacc gcgatgccgt agggcagctc
      241 gtcgccgagc aggcggaaga gcttctcgcg caggaattcg gaggcgagga agcgctcgct
      301 gcggtcggtg atctcgtctt cctcgaacat cggcgtaccg accggcagca ggggcgtggc
      361 ggccttgacc agctcgtcga cgttgcgtcc cttctcggcc gacagcggca cgatctcggt
      421 gaagtcgcgc gcctcgcgca cctgggcgat gaagggcagc aggcgggtct tgtccttcaa
      481 ctggtcgacc ttgttgatga ccaggatcac cttcgaggac tcggggatca ccgccagcac
      541 cttgcggtcg tcctcggtga agcgcccggc ttcgatgacg aagaacacca gatcgacgtc
      601 ggccagcacc tgcgacacgg tgcggttcat cgaacggttg agcgcgttgc ggtgatgggt
      661 ctggaagccg ggggtgtcca cgaacacgaa ctgcgcgccc ggctcggtga ggatgccggt
      721 gacgcggtgt cgggtggtct gcgccttgct ggagacgatg ctgatcttct gcccgatcag
      781 acggttgagc agcgtcgact tgccgacgtt ggggcgaccg acgatggcga cgaagccggc
      841 gcgaaagccg tcctcgccgg tgggctggtc gctgggggga gtctgggacg cgtcgctcat
      901 ttctttctca ccgcggcgag cgcgagctcg gccgactgtt gttcggcgat gcgccggctc
      961 gggccgcggc ccagcgtgcg taccgcgagt ttggggattt cgcaggcgac ctcgaactcc
     1021 tgcgcgtggg cttcgccgac gatctcgacc atggtgtagc tcggcaccgg aatgcggcgc
     1081 gcctgcagcc attcctgcag cgcggtcttg gggtccttgc tcggcttgca cggatcgact
     1141 tcggcgatca gcggtgcgta gaggcggtcg atgaccccct tcgccgcttc gaagccggcg
     1201 tccagataga ccgcggcgaa cagcgcttcg agcgcatcgg cgaggatgga cgggcgccgg
     1261 ctgccgccgg acttgagctc gccctcgccc aggcgcaggc agtcgccgag ctcgagctcg
     1321 agcgcgaggc ggtgcagcgt gtcctggcgc accagcgagg cgcgcacccg cgacagctct
     1381 ccctcgcgca gctcgccgaa gcggccaaac agcgcgatcg ataccacgca gtcgaggacg
     1441 gcgtcgccga ggaactcgaa acgctcgttg ttgggctgcc cgaagctgcg atgggtcagc
     1501 gcctcctgca gcaggcggac atcggagaac gcgtggccca ggcgcgcctg aagtcgatcc
     1561 ggcatcatcg gctcagcgcg gctcgctgct cggatgcagg tcgatcgaca ggctgacgtt
     1621 ggcgagcagg ggaatcttgc gggtgtactc gacgtcgatg acggtgcgga cacccgactt
     1681 gtcgatcgtc agatccgcgc caccgacgct gctcacgtcg tcgatctgcc cgcgccgatc
     1741 gaagctgcgc cgcacctcga tcaggctcgc ccccttgtcg gactcgtcgg cgacgattcc
     1801 gaggatgcgc ttgatggcga gatactcatt gatcaccgga acgctgcgga acccgacgac
     1861 gagcacgaag gccgcgatca cgccggtcac cagcacgccc agaagcgtga gcccgcgttg
     1921 ggaacgcatc ttcatctcca cccccatttc agtcgaagcc gccgatccgc ttcatgtcgc
     1981 cgaagttgaa ccagatgaag aaggcacgcc cgacgatgtt ctggtccggc acgaagcccc
     2041 acacgcggct gtcgctgctg gcgtcgcgat tgtcgccgag cacgaagtag tgcccggccg
     2101 gcaccgtgca gcgcacgccc gcatgcacgt aggtgcaatg ctcgcgcccg gggaagttga
     2161 gcacctgcgg cacgtaggcc ggcgcgtcgc gctcgatcag cacagagtgc tcgacctcgc
     2221 cgagcttctc gacgtatttc ggcgagtagt acagccgatc cggatgcagg tagctgccgt
     2281 cctcacggat cgccaccgcc tcgccgttga tggtgaggcg cttgtcgatg tattcgacgc
     2341 ggtcgccggg caggccgacc acgcgcttga tgtagtccat cgacggatcg gccggatagc
     2401 ggaagaccat gacgtcgccg cgctgcggat cgttcacggc gacgatcttg cggttgatca
     2461 ccggcaggcg gatcccgtag gtccacttgt tgaccaggat gaagtcgccc accagcaagg
     2521 tcgggatcat cgaaccggaa gggatcttga aaggctcgac gacgaaggag cgcaggccga
     2581 acacgatcag gatcaccggg aagaagctcg cgccgtactc cacccaccac gggtcgggcg
     2641 tgcccgcggg acgcagcttg cgcgcgtgaa agcggtcgac ggaccacagc gcgccggagg
     2701 cgatcagcaa caggaacagg atcagggcaa agttcatgcg gtttccgtcg tgggccggcc
     2761 cccagggccg gcgtcgatca cttgccttcg tcggtgcgca gcacggcgag gaaggcttcc
     2821 tgcgggatct cgacgttgcc gacctgcttc atgcgcttct tgccttcctt ctgcttctcg
     2881 agcagcttct tcttgcgcgt gatgtcgccg ccgtagcact t
//
LOCUS       DAOQNN010000400         2915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_24851_length_2915_cov_9.322727, whole genome shotgun sequence.
ACCESSION   DAOQNN010000400 DAOQNN010000000
VERSION     DAOQNN010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24851_length_2915_cov_9.322727"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /gene="dnaG"
                     /locus_tag="PKV97_15195"
     CDS             complement(<1..1318)
                     /gene="dnaG"
                     /locus_tag="PKV97_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNB07278.1"
                     /translation="MIPQSFVQELLSRVDIVDVIERYLPLKKSGANYFACCPFHGEKS
                     ASFSVSPSKQFYHCFGCGVHGSAVGFLMEYSGLGFVDAIKELAAQVGLQVPDDGRSAA
                     PVHDDASERLYEAMAVAARFYRDQLKPSRQAIDYLKRRGLSGEVAARFGLGYAPDEWQ
                     ALEKAFPDYEAKTLADAGLVIDNEQGRRYDRFRNRIIFPIHDRRGRIIAFGGRVLDKG
                     EPKYLNSPETPLFEKGRELYGLFLAQKAIRDAGFAVVVEGYMDVVALAQFAIENAVAT
                     LGTATTPQHIHTLLRQTDRIVFCFDGDAAGRRAAWRALENALESLRDDATLAFLFLPA
                     EHDPDSFVRAEGAEAFRKAALAATPLGSFLIQELSGRHPLDSAEGRAAFVHEAAPLVT
                     RIAAPLLKLQVVKAVAESAGMTQGEVEYAFEQLAASAPRRRGASAPT"
     gene            complement(1479..1691)
                     /gene="rpsU"
                     /locus_tag="PKV97_15200"
     CDS             complement(1479..1691)
                     /gene="rpsU"
                     /locus_tag="PKV97_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNB07279.1"
                     /translation="MPGIRVKENEPFEVAIRRFKRTIEKTGVLTELRSREFYEKPTAE
                     RKRKLAAAVKRNHKRLRSQTLPPKLY"
     gene            complement(1654..1845)
                     /locus_tag="PKV97_15205"
     CDS             complement(1654..1845)
                     /locus_tag="PKV97_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07280.1"
                     /translation="MPSTFIGMHPFDSVSHFNPPPPARAIGGLAIDGRLVYIRGLSAH
                     QTPKEVRNARYPRQGKRAV"
     gene            1829..2869
                     /gene="tsaD"
                     /locus_tag="PKV97_15210"
     CDS             1829..2869
                     /gene="tsaD"
                     /locus_tag="PKV97_15210"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNB07281.1"
                     /translation="MKVLGIETSCDETGVAIYDTDTGLRAHCLHSQIDLHAAYGGVVP
                     ELASRDHIRRLPLLLRQTLAAAGLAAADIDAVAYTSGPGLAGALLVGASVAESFAMAR
                     GIPALPVHHLEGHLLSPLLSADPPAFPFVALLVSGGHTQLMRVRGVGDYALLGESVDD
                     AAGEAFDKTAKLLGLGYPGGPQLAGLAESGVPGRFRLPRPMLHSGDLDFSFSGLKTAV
                     LNVVSAPDWDPARMADLAAEFQQAVVDVLCAKALAALKKVGLKTLVVAGGVGANRCLR
                     ATLDAALARRGGRVHYPEPALCTDNGAMIAFAGALRLAAGESVPEVCAVRIRPRWPMV
                     ELRPPVQAPAIL"
BASE COUNT          407 a          988 c         1077 g          443 t
ORIGIN      
        1 gggtgggcgc cgaggcgcca cgccggcgcg gggcgctggc ggcgagctgc tcgaaggcgt
       61 attcgacctc gccctgggtc atgcccgcgc tctcggccac cgccttcacc acctgcagct
      121 tcagcagcgg cgcggcgatg cgagtgacca gcggcgccgc ctcatgcacg aaggccgcgc
      181 ggccctcggc gctgtcgagc ggatggcggc cggacagctc ctggatcagg aagctcccca
      241 gcggggtggc ggcgagcgcg gccttgcgga aggcttcggc gccctcggcg cgcacgaagg
      301 agtccgggtc gtgctcggcg ggcaggaaca ggaaggccag ggtggcgtcg tcgcgcaggg
      361 attcgagtgc gttctcgagc gcacgccatg ccgcgcggcg gcctgcggcg tcgccgtcga
      421 agcagaacac gatgcggtcg gtctggcgca gcagggtgtg gatatgctgc ggcgtggtcg
      481 cggtgccgag cgtggcgacc gcattctcga tggcgaactg ggcgagcgcg accacgtcca
      541 tgtagccctc gaccacgacc gcgaagccgg cgtcgcggat cgccttctgc gccaggaaga
      601 ggccgtagag ctcgcgcccc ttctcgaaca gcggggtctc gggcgagttc aggtacttgg
      661 gttcgccctt gtcgagcacg cgcccgccga aggcgatgat gcgtccgcgg cggtcgtgga
      721 tcgggaagat gatgcggttg cggaagcggt cgtagcgccg cccctgctcg ttgtcgatga
      781 ccaggccggc gtcggcgagc gtcttcgcct cgtagtcggg gaaggccttc tccagcgcct
      841 gccactcgtc gggcgcgtag ccgagcccga agcgcgcggc gacctcgccc gacagcccgc
      901 ggcgcttgag gtagtcgatg gcctggcggg agggcttgag ctggtcgcgg tagaagcgcg
      961 ccgccaccgc catcgcctca taaaggcgtt cgctcgcgtc gtcatgcacg ggtgccgcgc
     1021 tgcggccatc gtcgggcacc tgcaggccga cctgggcagc gagctccttg atcgcgtcga
     1081 cgaagcccag cccgctgtac tccatcagga agccgacggc gctaccgtgc acgccgcaac
     1141 cgaagcagtg gtagaactgc ttggacgggc tgaccgagaa cgaggcggat ttctcgccgt
     1201 ggaaggggca gcaggcgaag tagttggcgc cgctcttctt gagcgggagg tagcgttcga
     1261 tgacgtcgac gatgtcgacg cgggaaagca gttcctggac gaaggactgg ggaatcatcg
     1321 cgcgcgcaaa gacaaacgcc cgtcgcttcc gcatgcgcgg acgacgggtg ttcgtgaaaa
     1381 agcatcacgc cgggcgggga tcgagcgacc ccgggccggc gtgtgatcgc ccgccagggc
     1441 gggcatgggc gtcccgcgag ggcgggtcgc gtaccggatc agtagagctt cggcggcagc
     1501 gtctggctgc gcaggcgctt gtggttgcgc ttgacggcgg cggccagctt gcgcttgcgc
     1561 tcggcggtgg gcttttcgta gaactcgcgc gagcgcagtt cggtcagcac accggtcttc
     1621 tcgatggtgc gcttgaagcg gcggatcgca acttcaaacg gctcgttttc cttgacgcgg
     1681 ataccgggca ttgcggactt ccttgggtgt ctggtgggca gaaagaccgc gaatataaac
     1741 cagccgaccg tcgatggcaa ggccgccgat cgcccgcgcc ggcggtggcg ggttaaaatg
     1801 cgacaccgaa tcaaacggat gcatcccgat gaaagtgctc ggcatcgaaa cctcctgcga
     1861 cgagaccggc gtggcgatct acgacaccga caccggcctg cgcgcgcact gcctgcactc
     1921 gcagatcgac ctgcacgcgg cctacggcgg ggtggtgccc gagctcgctt cgcgcgatca
     1981 catccgccgc ctgccgctgc tgctgcgcca gaccctggcc gcggccggcc tcgccgccgc
     2041 ggacatcgac gccgtggcct acaccagcgg tcccgggctc gcgggcgcgc tgctggtggg
     2101 ggcgagcgtg gcggagtcgt tcgcgatggc gcgcgggatc ccggcgctgc cggtgcatca
     2161 cctcgagggc cacctgctgt cgccgctgct gtcggccgat cctccggcct tccccttcgt
     2221 ggccttgctg gtttcgggcg gccacaccca gctgatgcgg gtgcgcgggg tgggcgacta
     2281 cgcgctgctc ggcgagtcgg tggacgacgc cgccggcgag gccttcgaca agaccgccaa
     2341 gctgctcggc ctgggctatc ccggcgggcc gcagctcgcc gggctggccg aatccggcgt
     2401 gcccggccgc ttccgcctgc cgcggccgat gctgcactcg ggggacctcg acttcagctt
     2461 cagcggtctc aagaccgcgg tgctcaacgt ggtgtcggcg cccgactggg acccggcgcg
     2521 catggccgac ctcgctgccg agtttcagca ggccgtggtc gatgtgctgt gcgcgaaggc
     2581 gctcgcggcg ctgaagaagg tggggctgaa gaccctcgta gtggccggcg gggtgggtgc
     2641 caaccgctgc ctgcgcgcca ccctggacgc cgcgctcgcg cgccgcggcg ggcgcgtgca
     2701 ttaccccgag ccggcgctgt gcaccgacaa cggcgccatg atcgccttcg ccggcgcctt
     2761 gcgcctggcg gccggcgagt ccgtgccgga agtctgcgcc gtccgcatcc gcccgcgctg
     2821 gcccatggtc gaactgcgcc cgccggtgca ggcgcccgcc atcctgtagg agcggcgcaa
     2881 gccgcgaacc cggcgcccgg catcagagcc gccgc
//
LOCUS       DAOQNN010000401         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_24892_length_2913_cov_10.452064, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000401 DAOQNN010000000
VERSION     DAOQNN010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24892_length_2913_cov_10.452064"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /locus_tag="PKV97_15215"
     CDS             <1..1737
                     /locus_tag="PKV97_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNB07282.1"
                     /translation="SFPFLKEIILFLALSGVLMPLLSRLKINPVLGFLSVGVLLGPYG
                     LASLAKAWPPLSWFTFARPDQVEFLAELGVIFLMFMIGLEMSVERLWSMRRLVFGLGG
                     LQVALSAAAVGGLALWFGNGIEAAVILGVVLAFSSTAIVMQLLIQRHELGTPLGQASF
                     AILLFQDLAVVPLLVLISILGAASGEGSFASLLGLAMLKGVLTVVGIYLVGRRVVRPL
                     FHQIAVDKQPDTFTALTLLTTLGVAALTWVAGLSMALGALLAGLIIAETEFRHEVEIT
                     IEPFKGLLMGLFFMSVGMGIDLRALLAEPLWIPLSVVGLLAMKALIVFVLLRVFGLSW
                     GRSAEGGLLLGQGGEFAFIVIGMALTLGLLERDVGQFMLIVVGVSMLAAPVVARLGQS
                     LGDGIDRRIAPPAEANDAELGELGGHVIIAGYGRVGQLVGQMLEEQGVGYVAIENNAQ
                     LIAHQRKAGVPLVFGDASRPELLRKLRLDQARAVVLTMDHTAAAIHAVHGIRRMVPMM
                     RIIARARDEKHALLLREAGASVVVPETLESSLKLTGWVLETLGIPPDAALRLVELERE
                     RRIVALRDTGNA"
     gene            1763..2785
                     /locus_tag="PKV97_15220"
     CDS             1763..2785
                     /locus_tag="PKV97_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNB07283.1"
                     /translation="MIPQHNALQILRKPRIALLATGGTIAGTAGSATDTRDYTAGQLG
                     PAALLAAVPQLAELARIDATQIFSVDSKDMTPQHWLVLSRRVQRLMDDPAVDGIVITH
                     GTDTLEETACFLNITVATTKPVVITAAMRPATALSADGPLNLYQAVELACRPAAARSG
                     VLAVIGDRILPAATLAKRHPSAVDAFAFEGALTTLASDAALHALAPSRYSRVVLPDEL
                     QHLPPVELLTVAAGSSPALASAVARALARAQLLNGAAGLVLALPGNASLPEAWRPALE
                     ELPNAVPVVLASRCGGGVRRHHGNAPANWLDAGALDPLKARVRLIAGLGAGLRGARLR
                     RWFERA"
BASE COUNT          365 a         1098 c          981 g          469 t
ORIGIN      
        1 agctttccct tcctcaagga aatcatcctc ttcctcgcgc tgagcggtgt gctgatgccg
       61 ctgctgtcgc gcctgaagat caaccccgtg ctcggcttcc tgtcggtcgg ggtgctgctc
      121 ggcccctacg gcctcgcctc gctcgcaaag gcctggccgc cgctgtcgtg gttcaccttc
      181 gcgcgccccg accaggtgga gttcctcgcc gagctcggag tcatcttcct gatgttcatg
      241 atcggcctgg agatgtcggt cgagcgcctg tggtcgatgc gccggctggt gttcgggctc
      301 ggcggcctgc aggtcgcgct ctcggcagcg gcggtcgggg ggctcgcgct gtggttcggc
      361 aacggcatcg aggccgcggt catactcggc gtggtgctgg ccttctcgtc caccgcgatc
      421 gtcatgcagt tgctgatcca gcgccacgaa ctcggcacgc cgctcggcca ggcctcgttc
      481 gcgatcctgc tgttccagga cctcgcggtg gtgccgctgc tggtgctgat cagcatcctc
      541 ggtgccgcct cgggcgaggg cagcttcgcg agcctgctcg gcctcgcgat gctcaagggc
      601 gtgctcaccg tggtgggcat ctacctcgtc ggtcgccgcg tggtccgtcc gctcttccac
      661 cagatcgcag tcgacaagca acccgacacc ttcaccgcgc tcaccctgct gaccaccttg
      721 ggcgtggcgg cgctgacctg ggtggccggc ctgtcgatgg cgctcggcgc cctgctcgcc
      781 ggcctgatca ttgccgagac cgagttccgc cacgaggtgg agatcaccat cgagcccttc
      841 aagggcctgc tgatgggcct gttcttcatg tcggtgggca tgggcatcga cctgcgcgcg
      901 ctgctcgccg agcccttgtg gattccgctc tcggtggtcg gtctgctggc catgaaggcg
      961 ctcatcgtgt tcgtgctgct gcgcgttttc ggcctgtcct gggggcgctc cgccgagggc
     1021 ggcctgctgc tcggccaagg cggcgagttc gccttcatcg tgatcggcat ggcgctcacg
     1081 ctgggcctgc tcgagcgcga tgtgggccag ttcatgctga tcgtggtcgg cgtgtcgatg
     1141 ctcgccgcgc cggtggtcgc acgcctcgga cagagcctgg gcgacggcat cgaccgacgc
     1201 atcgcgccgc cggccgaggc gaacgatgcc gagctcggcg agctcggcgg gcatgtgatc
     1261 atcgccggct acggccgcgt cggccagctg gtcgggcaga tgctggaaga gcagggcgtg
     1321 ggctacgtcg ccatcgagaa caatgcccag ctcatcgccc accagcgcaa ggcgggggtg
     1381 ccgctggtgt tcggcgacgc cagccgcccg gagctgctgc gcaagctgcg cctcgaccag
     1441 gcgcgcgcgg tggtgctgac catggaccac accgccgccg cgatccacgc ggtgcacggc
     1501 atccgccgca tggtgccgat gatgcggatc atcgcccgcg cgcgcgacga gaagcatgcc
     1561 ctgctgctgc gcgaggccgg cgcctcggtg gtggtgcccg aaaccctcga gtccagcctc
     1621 aagctcaccg gctgggtgct ggagaccctc ggcatacccc ccgatgccgc gctacgcctg
     1681 gtcgagctgg aacgcgagcg gcgcatcgtc gcgctgcgcg acaccggcaa cgcctgaccg
     1741 ccccctgacg aatcccgccc acatgatccc gcagcacaac gccctccaga tcctgcgcaa
     1801 gccgcgcatc gcgctcctcg ccaccggcgg caccatcgcc ggcaccgccg gctcggccac
     1861 cgacacccgc gattacaccg ccggccagct cggccccgcg gcgctgctcg ccgccgtgcc
     1921 gcagctggcc gagctcgccc gcatcgacgc cacccagatc ttcagcgtcg acagcaagga
     1981 catgacgcca cagcactggc tggtgctgtc gcgccgggtg cagaggctga tggacgaccc
     2041 cgcggtcgac ggcatcgtca tcacccacgg caccgacacg ctggaagaga cggcctgttt
     2101 cctcaacatc accgtcgcca ccaccaagcc ggtggtgatc acggcggcga tgcgcccggc
     2161 caccgcgctt tcggccgacg gcccgctcaa cctctaccag gcggtcgagc tcgcctgccg
     2221 cccggcggcc gcacgctcgg gcgtgctcgc ggtgatcggc gaccgcatcc tgcccgcggc
     2281 caccctcgcc aagcgccatc cctcggcggt cgatgcgttc gcgttcgagg gcgcgctcac
     2341 cacgctcgcc agcgacgccg cgctgcacgc gctcgcgccc tcgcgctaca gccgtgtcgt
     2401 gctgccggac gagttgcagc acctgccgcc ggtggagctg ctcaccgtcg ctgcgggcag
     2461 ctcgcccgca ctcgcctcgg cggtggcgcg cgcgctcgcg cgcgcccagc tgctcaatgg
     2521 cgccgcaggc ctggtgctgg ccctgcccgg caacgccagc ctgcccgagg cctggcgacc
     2581 cgcgctggaa gaactgccca acgcggtacc ggtggtgctc gccagccgct gcggcggcgg
     2641 cgtgcgtcgg caccatggca acgcccccgc caactggctc gacgccggcg cgctcgaccc
     2701 gctcaaggcg cgcgtgcgcc tgatcgcggg cctcggcgcc ggattgcgcg gcgcacgtct
     2761 gcggcgctgg ttcgagcgcg cctgacgcgg cggtgcttgc gtcgcccccg cgcgcccctc
     2821 acagccccgg atcggtgccg ccgccggcga gcgcggtctc gaccatgcgc cgcgacagat
     2881 gcggcgcgaa gccctcgatg aaggcgtaca cat
//
LOCUS       DAOQNN010000402         2912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_24910_length_2912_cov_7.975149, whole genome shotgun sequence.
ACCESSION   DAOQNN010000402 DAOQNN010000000
VERSION     DAOQNN010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24910_length_2912_cov_7.975149"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKV97_15225"
     CDS             complement(<1..444)
                     /locus_tag="PKV97_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNB07284.1"
                     /translation="MNEAKDVEHSPWAVFLIFLRLGLTSFGGPVAHLGYFHAEFVARR
                     RWLGERAYADLVALCQFLPGPASSQVGMALGLQRAGYAGAFAAWAGFTLPSAIALILF
                     ALGLARHGDALPSGALHGLKVVAVVAQAVWGMAGKLCIGRARITLM"
     gene            complement(584..1477)
                     /locus_tag="PKV97_15230"
     CDS             complement(584..1477)
                     /locus_tag="PKV97_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNB07285.1"
                     /translation="MDGEDLGAGGCCGTCGGAAPKPRLQPLAAAEARTRSVFRIPGMD
                     CPSEEQMIRLRLAEAPVAALAFDLAGRRLTVDHDGDPQAILARLVPLGYGAELAESHT
                     LAADEAAPAAPADDAAEARVLWILLALNAVMFVVELGAGIWARSAGLVADAMDMFADA
                     AVYGVALYAVGRAARYKLGAARLAGGLQLVLALGALAEVVRRIVGDATPEPIGMMGIA
                     LLALAANVACLVLIARHREGGVHMKASYIFSANDVVANLGVIVAGVLVAWTGSAWPDW
                     IIGLVIGAVVLTGAVRILRLG"
     gene            complement(1506..1940)
                     /locus_tag="PKV97_15235"
     CDS             complement(1506..1940)
                     /locus_tag="PKV97_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNB07286.1"
                     /translation="MPIFWRPQFRIGHDDIDADHRYLILLINTVELVLRFPDDPRNVE
                     LALTELRRYAEDHFEREERIQIAYGYLHLDQHKLEHRALLAELDILVQRIHATLEDSD
                     GGVEGVKAQSGEITAFLRKWLVDHVMKSDMQLVPLFRKPVVR"
     gene            complement(2015..2644)
                     /locus_tag="PKV97_15240"
     CDS             complement(2015..2644)
                     /locus_tag="PKV97_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HNB07287.1"
                     /translation="MPTLDRRQFSPSAARNRDPILAVLQHVLPTAGLVLELGSGTGEH
                     AVHFARHLPALRWQPSDADPAALASITDWVAHSALPNVLSPLCFDLSTMPWPVAAADA
                     IVAINVLHYSPWESTPALFAGAAAVVPEGGVVVCYGPYRRGGAHTAPSNADFDEWLRS
                     VDARFAVRDLEAVETEARRCGFGLEAVVDMPANNFSLVFRRGGGLGVRA"
BASE COUNT          487 a         1051 c          998 g          376 t
ORIGIN      
        1 catcagggtg atgcgggcgc ggccgatgca gagcttgccc gccatgcccc acaccgcctg
       61 cgccaccacc gccaccacct tcagcccgtg cagcgcgccc gacggcagcg cgtcgccatg
      121 tcgtgcgaga ccgagcgcga agaggatcag cgcgatcgcc gagggcagcg tgaagcccgc
      181 ccacgcggcg aaggcgccgg cgtagccggc gcgctgcagc ccgagcgcca tgcccacctg
      241 gctgctcgcc gggccgggca ggaactggca cagcgccacc agatcggcat aggcgcgctc
      301 acccagccag cgccggcgcg cgacgaactc ggcgtggaaa tagcccaggt gcgccaccgg
      361 cccgccaaac gacgtgaggc ccaggcgcag gaagatcagg aagaccgccc aggggctgtg
      421 ctcgacgtct ttcgcttcgt tcatgtcggc tttcacagaa gttgatcggg tcttgcagga
      481 tgctcggagc cggcgtcatc aggcgcaggc gcatgccccc gcatcgacgg ggcccggccg
      541 acgaccgcgc actgccgatg gcgtaccggc cccccggtgg cgttcaccca agtcgcagga
      601 tgcgcaccgc gcccgtcagc accaccgcgc cgatcaccag gccgatgatc cagtccggcc
      661 acgcgctgcc ggtccacgcc accagcacgc cggcgacgat cacgccgagg ttggcgacga
      721 cgtcgttggc cgagaagatg tagctcgctt tcatgtgcac tccgccctcg cgatggcggg
      781 cgatcagcac caggcaggcg acgttggcgg cgagcgcgag cagcgcgatg cccatcatgc
      841 cgatcggctc cggcgtggca tcgccgacga tgcgacgcac gacctcggca agcgcgccaa
      901 gggccagcac cagctgcagc ccccccgcca ggcgcgcggc acccagcttg tagcgcgcgg
      961 cccgtcctac cgcgtaaagc gcgacgccat agacggcggc gtcggcgaac atgtccatcg
     1021 cgtcggcaac caggccggcc gagcgcgccc agatgccggc gccgagttcg accacgaaca
     1081 tcacggcatt gagcgccagc aggatccaca gcacgcgggc ctcggccgcg tcgtcggcgg
     1141 gcgcggcggg cgcggcctca tcggcggcga gcgtatgcga ctccgccagc tcggcgccgt
     1201 aacccagcgg gacgagacgg gcgaggatcg cctgcgggtc gccgtcgtga tccaccgtca
     1261 gccgccgccc ggcgaggtcg aacgccagcg ccgccaccgg cgcctcggcc aggcgcaggc
     1321 ggatcatctg ctcctcggac gggcagtcca tgccggggat gcgaaacacg ctgcgcgtgc
     1381 gcgcctcggc ggccgccagc ggctgcagcc gcggcttggg cgccgcgcca ccgcacgtgc
     1441 cgcaacagcc gcccgcacca agatcttctc catccatcga gggctgctcg ctcaaagcgg
     1501 agctcctaac gcacgaccgg cttgcggaac agcggcacga gctgcatgtc cgacttcatc
     1561 acgtgatcca ccagccattt gcgcagaaag gcggtgatct cgccgctctg cgccttgaca
     1621 ccttccaccc cgccatcgga atcctcgagc gtggcgtgga tgcgctgcac gaggatgtcg
     1681 agctcggcca gcaaggcgcg gtgttcgagc ttgtgctggt cgaggtggag gtagccgtag
     1741 gcgatctgga tgcgctcctc gcgctcgaag tggtcctctg cgtagcgtcg cagctcggtc
     1801 agcgccagct cgacattgcg cgggtcgtcc ggaaagcgca gcacgagctc caccgtgttg
     1861 atgagcagga tcaggtagcg gtggtcggcg tcgatgtcgt cgtggccaat gcggaattgc
     1921 ggtcgccaga agatgggcat gggagcctcg ggaacggttc gggggcgtgg aaacgcatca
     1981 gcatcgattg tcgccgatgc gccggccgcg cacatcatgc gcgcacgccg agccccccac
     2041 cgcgacggaa gaccaggctg aagttgttcg ccggcatgtc gaccaccgcc tccagcccga
     2101 agccacaacg ccgggcctcg gtctccaccg cctccaggtc gcgcaccgcg aagcgcgcat
     2161 cgacgctgcg cagccattcg tcgaagtcgg cgttgctcgg cgcggtgtgg gcgccgccgc
     2221 ggcgatacgg gccgtagcag accaccacgc cgccttccgg cacgaccgcc gccgcgcccg
     2281 caaacagcgc cggcgtgctc tcccagggcg agtaatgcag cacgttgatc gccacgatgg
     2341 cgtcggcggc ggcgaccggc cagggcatgg tggaaaggtc gaagcacagc ggcgacagca
     2401 cattgggcag cgcgctgtgc gccacccagt cggtgatcga ggccagcgcc gcgggatcgg
     2461 catcgctcgg ctgccaacgc agcgcgggca ggtggcgggc aaaatgcacc gcatgctcgc
     2521 cggtgccgct gccgagctcg agcaccaggc ccgcggtcgg cagcacgtgc tgcagcacgg
     2581 ccaggatggg gtcgcggttg cgcgcggcgg agggcgagaa ctggcggcgg tcgagggtcg
     2641 gcatgaggcg cggcgggcag ggtgaaggac ttcgtgatta tcgcctgctc tgcgccgggc
     2701 gccggcaatt ccagcccgga cgattcggac cgggcgtccc ctccgcctcg ccgtcagagc
     2761 gcagcggact tacatcgccc ttgtcgatgg aatcgctcgg gtaaaagata gatgcgcaaa
     2821 cgcgcacgcc gaggctgaga taatgaccca ccctcttcac cgccctaccc caccatgacc
     2881 gcacccgcca aacccctcgc cggcatcaag gt
//
LOCUS       DAOQNN010000403         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_24990_length_2906_cov_5.638723, whole genome shotgun sequence.
ACCESSION   DAOQNN010000403 DAOQNN010000000
VERSION     DAOQNN010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24990_length_2906_cov_5.638723"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /gene="hypD"
                     /locus_tag="PKV97_15245"
     CDS             <1..1022
                     /gene="hypD"
                     /locus_tag="PKV97_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNB07288.1"
                     /translation="NVRMIHGPGCPVCVLPIGRVDMAIGLALARPEVILCSYGDCLRV
                     PASDGLSLLKAKARGADVRMVYSAADALALAVREPAREVVFFAIGFETTTPPTALVLR
                     EAATRRLANFSVICNHVLTPPAITAILDSPAASRLGALHLDGFIGPAQVSTLIGSRPY
                     EAFAARYRKPVVIAGFEPLDVMQAIRMLVRQVDTGRAEVENEFTRAVVRDGNPKAQAI
                     VSEVLALRDEFEWRGLGVLARSALRIRPAFAPWDAEAKFGLDYRPVPDHRACECGAIL
                     RGVKKPTDCKLFGTVCTPETPMGSCMVSSEGACAAHYNYGRFRDVPVHVVGTSAPPNP
                     DQETA"
     gene            1019..2080
                     /gene="hypE"
                     /locus_tag="PKV97_15250"
     CDS             1019..2080
                     /gene="hypE"
                     /locus_tag="PKV97_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNB07289.1"
                     /translation="MNTVKTGYVRPLDLRKGRVDLGFGAGGRAMAQLISELFLRAFAN
                     DWLARGDDGAVLPAPAAGERLVMATDAHVVSPLFFPGGDIGSLSVHGTVNDLAVMGAR
                     PLYLAASFILEEGYALADLARIVESMASAARAAGVTVVTGDTKVVEQGKGDGVFITTT
                     GVGALPAGRDPGGARARPGDVVLVSGRIGDHGMAIMAQRESLAFDSEIVSDSAALHDL
                     VEALYAAVPAEAVRVLRDPTRGGLATTLNEIAAQSGVGMELDEAAIPVSAQVQAACEL
                     LGLDPLYVANEGKLVVLCAPEHAGAALAALRAHPLGTEAARIGCATADPQHFVQLRTG
                     LGGRRMVDWIAGEQLPRIC"
     gene            2246..>2906
                     /locus_tag="PKV97_15255"
     CDS             2246..>2906
                     /locus_tag="PKV97_15255"
                     /inference="COORDINATES: protein motif:HMM:NF024189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HNB07290.1"
                     /translation="MTIRSPLSPMLLAAALALGGCAVVPVDDYGYYEDDRYYERETVI
                     VTPPPRIEYRGLPPAAGYIWIDGYWNRVGPRPHWVPGYWSPPRMHSRPLAQPPHIDVR
                     PDGRGDRRDEHDRNRWRSDDARRDDRRWNDRSRDDVRRDRDSRPWPGFSPRGDGAGSP
                     DGPQLRDRRDLRDEARPPRIFQERPPGGREGLLRAEPPRDRAARPELRPAGAAGEARE
                     PR"
BASE COUNT          395 a         1106 c         1012 g          393 t
ORIGIN      
        1 ccaacgtgcg catgatccac ggccccggct gtccggtgtg cgtgctgccg atcggtcgcg
       61 tggacatggc gatcggcctc gcgctggcgc gccccgaggt gatcctgtgc agctacggcg
      121 actgcctgcg cgtgcccgcc tccgacggcc tttcgctgct gaaggccaag gcacgcggcg
      181 ccgacgtgcg catggtgtac tccgccgccg acgcgctcgc gctcgccgtg cgcgaacccg
      241 cacgcgaggt ggtcttcttc gcgatcggct tcgagaccac cacgcccccc accgcgctcg
      301 tgctgcgcga ggccgccacg cgcaggctcg ccaacttcag cgtgatctgc aaccacgtgc
      361 tgacgccgcc ggcgatcacc gcgatcctcg actcgcccgc agccagccgg ctcggggcgc
      421 tgcacctgga cgggttcatc ggccccgccc aagtgtccac cctgatcggc agccgcccct
      481 acgaagcctt cgccgcccgc taccgcaagc ccgtggtcat cgccggcttc gaaccgctcg
      541 atgtgatgca ggcgatccgc atgctggtgc gccaggtcga cacgggccgt gccgaggtcg
      601 aaaacgagtt cacccgcgcg gtggtgcgcg acggcaaccc gaaagcgcag gcgatcgtgt
      661 cggaggtgct ggcgttgcgc gacgagttcg agtggcgcgg cctcggtgtg ctggcgcgct
      721 ccgcgctgcg catccgcccc gccttcgcgc cctgggacgc cgaggcgaag ttcggtctcg
      781 actaccgccc cgtgcccgac caccgcgcct gcgaatgcgg cgccatcctg cgcggcgtga
      841 agaagccgac cgactgcaag ctcttcggca ccgtgtgcac cccggaaacg ccgatgggct
      901 cgtgcatggt gtcctccgag ggcgcctgcg ccgcgcacta caactacggg cgcttccgcg
      961 acgttccggt acatgtcgtc ggcacctccg cacccccgaa tccggaccag gaaaccgcat
     1021 gaacaccgtc aaaaccggct acgtccgccc cctggacctg cgcaagggcc gcgtcgacct
     1081 cggcttcggc gccggcgggc gggcgatggc gcaattgatc tccgaacttt tcctgcgcgc
     1141 gttcgccaac gactggctcg cccgcggcga cgacggcgcg gtgctgcccg cgcccgcagc
     1201 gggcgagcgc ctggtgatgg ccaccgacgc ccacgtggtg agcccgctgt tcttccccgg
     1261 cggcgacatc ggcagcctgt cggtgcatgg gacggtgaac gacctcgcgg tgatgggcgc
     1321 gcgcccactg tacctggccg ccagcttcat cctggaagaa ggctatgcgc tcgccgacct
     1381 cgcccgcatc gtcgaatcga tggcctccgc ggcgcgcgcg gccggggtga cggtggtgac
     1441 cggcgacacc aaggtggtcg aacagggcaa gggcgacggc gtgttcatca ccaccaccgg
     1501 cgtgggggcg ctgccggccg ggcgcgatcc gggcggcgcg cgggcgcggc cgggcgacgt
     1561 ggtgctggtg tcggggcgca tcggcgacca tggcatggca atcatggcgc agcgcgagtc
     1621 gctcgccttc gactccgaga tcgtctccga cagcgcggca ctgcacgacc tggtcgaggc
     1681 gctctacgcc gcggtgccgg ccgaggcggt ccgcgtgctg cgcgacccca cgcgaggcgg
     1741 actggcgacc accttgaacg agatcgccgc gcagtcgggc gtgggcatgg agctcgacga
     1801 ggcggcgatc ccggtgtcgg cgcaggtgca ggctgcctgc gagctgctcg ggctcgaccc
     1861 gctctacgtc gccaacgagg gcaagctggt ggtgctgtgc gctccggagc acgccggcgc
     1921 ggcgctcgcc gcgctgcgcg cgcatccgct cggcaccgag gcggcgcgga tcggatgcgc
     1981 caccgccgac ccgcagcact tcgtgcagct gcgcaccggc ctgggcgggc ggcgcatggt
     2041 ggactggatc gccggcgagc agctgccgcg gatctgttga gcgatcgcgt cgtcacaact
     2101 gattacctcg ccgcccaccc ggcgcgcccc ctttcgccta tcgtgcgcgt ggagacagca
     2161 gagacgcgga agggggcggc gggcggcagc gccccgcccc cggccgcccc gcatgtcgca
     2221 cccttctccg tggcaggacg caaccatgac catccgctcg cccctttcgc ccatgctgct
     2281 cgcagccgcg ctggcactcg gcggatgcgc ggtggtcccg gtcgacgact acggctacta
     2341 cgaagacgac cgctactacg agcgcgagac ggtgatcgtc acgccgcccc cccgcatcga
     2401 gtaccgcggc ctgccaccgg ccgccggcta catctggatc gatggctact ggaaccgggt
     2461 cggaccgcgc ccgcactggg tcccgggcta ctggagccca ccgcgcatgc attcgcgacc
     2521 gctcgcgcag ccgccgcaca tcgatgtgcg cccggacggg cgcggcgacc gtcgcgacga
     2581 acacgaccgc aaccgctggc ggagcgacga cgcgcgacgg gacgaccggc gctggaacga
     2641 ccgcagccgc gacgatgtcc gccgcgaccg cgacagccgg ccctggcccg gcttctcccc
     2701 ccgtggcgac ggcgctggtt ctcccgatgg cccgcagctg cgcgatcgcc gcgacctgcg
     2761 cgacgaggcc cgccccccga ggatcttcca ggaacgcccg cccggtggac gtgaaggact
     2821 gctgcgcgcc gaaccgccgc gcgatcgcgc tgcgcgcccc gagctgcgcc ccgcaggtgc
     2881 cgcaggcgag gcgcgcgagc cccgcc
//
LOCUS       DAOQNN010000404         2897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_25118_length_2897_cov_9.631597, whole genome shotgun sequence.
ACCESSION   DAOQNN010000404 DAOQNN010000000
VERSION     DAOQNN010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25118_length_2897_cov_9.631597"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKV97_15260"
     CDS             complement(<1..211)
                     /locus_tag="PKV97_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNB07291.1"
                     /translation="MASSPDSISMALFCDFENVALGVRDAKYEKFDIKRVLERLLLKG
                     SIVVKKAYCDWDRYKSFKAAMHEANF"
     gene            363..893
                     /locus_tag="PKV97_15265"
     CDS             363..893
                     /locus_tag="PKV97_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HNB07292.1"
                     /translation="MIKPAALAAALLATTLALPALAAERASARLLDRQGAEVGTATLV
                     EGPHGVLVHVVAKGLPPGPKAIHIHSVGTCEDGAQGFVASKGHLNPAARPHGLMNPDG
                     PDAGDLPNLFVHADGGVEAEFFTALASLSGAGGRAAILDADGAALVIHENRDDHASQP
                     IGGAGGRFFCGVIRAQ"
     gene            959..1489
                     /locus_tag="PKV97_15270"
     CDS             959..1489
                     /locus_tag="PKV97_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07293.1"
                     /translation="MASALVLTLAAAGCAPPTEEAALTPRGIGPMLLGVPLADAARAT
                     RRFDPAAARIGPGCDERDQIGVVLRVAGESLSVMAMAGADARIEEILAIPAQGAGAVT
                     LADAEACRAHGAAYAASLTPRLGAAPTWSVRERPVSHEFVFAFAGDVRVVARWFSGGR
                     SCDLLLQYGGKPAASP"
     gene            1683..2108
                     /gene="dksA"
                     /locus_tag="PKV97_15275"
     CDS             1683..2108
                     /gene="dksA"
                     /locus_tag="PKV97_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNB07294.1"
                     /translation="MAEDTLHKQFPPYVPAAGEEYMSEKQLAHFRAILDSLKGELVDD
                     IERTVHTMQDEATVFADPNDRASQESDIALELRNRDRERKLIKKIDETIGRIEAGEYG
                     YCDSCGVEIGLKRLEARPTATLCIDCKTLEEMRERQVAK"
     gene            complement(2226..>2897)
                     /locus_tag="PKV97_15280"
     CDS             complement(2226..>2897)
                     /locus_tag="PKV97_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNB07295.1"
                     /translation="AVLTDILGDEDHLGDMDFKVAGTENGITGLQMDIKIQGITKEIM
                     HAALEQAKEGRLHILGLMQQALGTSRGQVSEYAPRMINMKINPEKIRDVIGKGGAVIR
                     ALQEETGTVIEIEDDGSVKISSVSAEGAQAAKARIEAITAEVEVGKVYEGPVVRLLDF
                     GAIVNIMPGRDGLLHVSQIANERVNNVSDYVKEGQVVRVKVLETDERGKIRLSMKALL
                     NENAG"
BASE COUNT          421 a         1049 c          953 g          474 t
ORIGIN      
        1 cgaagttggc ctcgtgcatc gccgccttga agctcttgta gcggtcccag tcgcagtagg
       61 ccttcttgac cacgatgctg cccttgagca gcaggcgctc gagcacccgc ttgatgtcga
      121 acttctcgta cttcgcgtcc cgcacgccca gcgccacgtt ctcgaagtcg cagaacagcg
      181 ccatgctgat gctgtcgggg gaggaagcca tggaaatctc cgtttctgat gagccgccga
      241 tgttaagccc tcgccgcggt gcgtgccacc ggatgtaccg ccgcggcccg ccttggggcg
      301 tgcatgcctg ccggtttgtc ctatgcttga gactctggac gcattccaac aggagaacag
      361 ccatgatcaa acccgccgcc ctcgccgccg cgctccttgc caccacgctc gccctgcccg
      421 ccctcgccgc cgagcgtgcg agcgcccgtt tgctcgatcg ccagggcgcc gaggtgggca
      481 ccgcgaccct ggtcgagggg ccccacggcg tgttggtcca cgtggtcgcc aaggggctgc
      541 cgcctgggcc gaaggcgatc cacatccatt cggtgggcac ctgcgaggac ggcgcgcagg
      601 gcttcgtcgc ctcgaagggc cacctgaacc cggcggcaag gccgcacggg ctgatgaacc
      661 cggatggccc cgacgccggc gacctgccca acctcttcgt gcatgccgac ggcggcgtcg
      721 aggcggagtt cttcaccgcc ctcgccagcc tctcgggcgc gggcggacgc gccgccatcc
      781 tcgacgccga cggcgccgcg ctggtgatcc acgagaaccg cgacgaccac gccagtcagc
      841 cgatcggcgg cgccggcggg cgcttcttct gcggtgtgat ccgggcgcag tgagcggatc
      901 cgatcgccgc agccgttgcg aagccgggtc gcgcgcccac ccgcccgccg ccctcgcaat
      961 ggcgtcggcg ctcgtcctga cgctcgccgc cgcgggctgt gcgccaccca ccgaagaggc
     1021 ggcgctcacc ccccgcggca tcggtccgat gttgctcggc gttccgcttg ccgacgccgc
     1081 gcgcgccacc cggcgcttcg atcctgccgc cgcacggatc ggcccgggct gcgacgaacg
     1141 cgaccagatc ggcgtggtgc tgcgtgtggc cggcgagtcg ctgtcggtga tggcgatggc
     1201 cggcgccgac gccaggatcg aggagatcct cgccatcccc gcgcagggcg ccggcgcggt
     1261 cacgctggcc gatgccgagg cctgccgtgc gcacggcgcc gcctacgcgg ccagcctcac
     1321 cccccggctc ggcgcggcgc ccacctggtc ggtgcgcgaa cgcccggtct cgcacgagtt
     1381 cgtcttcgcc ttcgccggcg acgtacgcgt ggtggcgcgc tggttttccg gcggcaggag
     1441 ctgcgacctg ctgctgcagt acggcggcaa acccgccgcc agcccctgag caccgcgacc
     1501 gtccggggcc ctggcggcgc ctccgccgcg gctccgcagc gcggcagccg cgcactcgca
     1561 gccgggaacg cgcggtccga cacggggcac gccagggcga tccggggtat gcttgctgca
     1621 ccgggttgat tctgttatta agtcggacct caccaatcaa caccctctac ggaagtcgat
     1681 ccatggctga agataccctg cacaagcaat tcccgcccta cgttcccgct gccggcgagg
     1741 aatacatgag cgagaagcag ctggcccact tccgcgcgat cctcgattcg ctcaagggcg
     1801 agctggtgga tgacatcgag cgcacggtgc acacgatgca ggacgaggcg accgtgttcg
     1861 ccgaccccaa cgaccgcgcc agccaggagt ccgacatcgc gctcgagctg cgcaaccgcg
     1921 accgcgagcg caagctgatc aagaagatcg acgagacgat cggtcgcatc gaggccggcg
     1981 aatacggcta ctgcgacagc tgcggcgtcg agatcggcct caagcgcctc gaggcgcgcc
     2041 cgaccgcgac cttgtgcatc gactgcaaga cgctggaaga gatgcgcgag cgccaggtcg
     2101 cgaagtaatc cgccgctccc ggtcgcgacc gggacggaca acaaaaaagg acgcctgggg
     2161 cgtccttttt tgttggctgc gcgccgtgct gcagcgcgtg gcgggccgca gcccgccatc
     2221 cgccatcagc cggcgttttc gttcagcagc gccttcatgc tcaggcggat cttgccgcgc
     2281 tcgtcggtct cgaggacctt cacgcgcacg acctggcctt ccttgacgta gtccgagacg
     2341 ttattcacgc gctcgttggc gatctgcgac acgtgcagca ggccgtcgcg gcccggcatg
     2401 atgttcacga tggcgccgaa gtcgagcagg cgcacgaccg gaccttcgta gaccttgccg
     2461 acctcgacct cggcggtgat cgcctcgatc ctggccttgg ccgcctgggc gccctcggcg
     2521 ctgaccgagg aaatcttgac cgagccgtcg tcctcgatct cgatcacggt gccggtctct
     2581 tcctgcagcg cgcggatcac cgcgccgccc ttgccgatca cgtcgcggat cttctcgggg
     2641 ttgatcttca tgttgatcat gcgcggagcg tactcggaca cctggccgcg gctggtgccc
     2701 agggcctgct gcatgaggcc gaggatgtgc aggcggcctt ccttcgcctg ctccagcgcg
     2761 gcgtgcatga tctccttggt gatgccctgg atcttgatgt ccatctgcag gccggtgatg
     2821 ccgttctcgg taccggcgac cttgaagtcc atgtcgccga ggtggtcctc gtcgcccagg
     2881 atgtcggtca gcaccgc
//
LOCUS       DAOQNN010000405         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_25330_length_2882_cov_11.160948, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000405 DAOQNN010000000
VERSION     DAOQNN010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25330_length_2882_cov_11.160948"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /gene="dnaX"
                     /locus_tag="PKV97_15285"
     CDS             complement(<1..1153)
                     /gene="dnaX"
                     /locus_tag="PKV97_15285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNB07296.1"
                     /translation="MSYQVLARKWRPKSFGTLVGQEHVVRALTHALATGRLHHAWLFT
                     GTRGVGKTTISRILAKALNCETGITAEPCGICDACRAIDADRFPDYVEMDAASNRGVE
                     EMAALLDKAVYAPVQGRFKVYMIDEVHMLTGHAFNAMLKTLEEPPEHVKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMPQGHIVDHLTRILQAEEVAFEPGALRHLAKAAAGSMRDAL
                     SLLDQAIAHGAGRVEEEQVSHMLGTVGDDHLYAVLDALAAGDVPALLAVADGMEARSL
                     SFDAALQALATLVHRVALAQYAPAAIADELERARILGYVERFDAEFLQLAYQVAVHGR
                     DELPLAPDDYTGFTMTLLRLHAFRPEQPAALGGPGVPSGPAMASLKNALA"
     gene            complement(1212..>2882)
                     /gene="cysC"
                     /locus_tag="PKV97_15290"
     CDS             complement(1212..>2882)
                     /gene="cysC"
                     /locus_tag="PKV97_15290"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNB07297.1"
                     /translation="AEREQGITIDVAYRFFSTDKRKFIVADTPGHEQYTRNMVTGAST
                     ADAAILMVDARKGILTQTRRHSYLVNLIGIRHIVVAINKMDLVDYAEGVFRRIVEDYT
                     AFSRQLGIEQVTFIPMSAFRGDNITSPSAAMPWYHGTTLMGYLETVEVDDGLMQRAPF
                     RLPVQWVNRPNLDFRGFAGSIAGGTIRPGDRVRVQPSGRESTVARIVTRDGDLDRAVA
                     GQSVTLTLADEIDISRGDVISTVEAPAEVADQFECTVVWMHDEPMLAGRPYLLKIGAR
                     TVSATITEIKYQVNVNTLEHVAAKRLELNAIGVCNLSLDRPIAFDPYRINRDTGGFIL
                     IDRLSNNTVGAGLLHFALRRAHNIHLQHVDVDKRARAALKNQRGCVLWFTGLSGAGKS
                     SIANLVEKKLHALGHHTYLLDGDNVRHGLNKDLGFTDADRVENIRRVAEVAKLMVDAG
                     LIVLTAFISPFRSERRMARGLVEEGEFVEVFVDTPLEVAEARDPKGLYKKARRGELKN
                     FTGIDSPYEAPEDPELRLDTTRLDLEAAADAVLAWLGERGMLSRTPGG"
BASE COUNT          439 a          936 c         1038 g          469 t
ORIGIN      
        1 cggccagcgc gttcttcaat gacgccatcg ccggcccgga tggcacgccc ggcccgccaa
       61 gcgcagcagg ctgctccggg cggaacgcat gcaggcgcag cagggtcatc gtgaagccgg
      121 tgtagtcgtc cggcgccagc ggcagctcgt cgcggccgtg gacggcgacc tggtaggcga
      181 gctggaggaa ctcggcgtcg aagcgctcga cgtagccgag gatgcgcgcg cgctcgagct
      241 cgtcggcgat cgccgcaggt gcgtactggg cgagcgcgac gcgatgcacc agcgtggcca
      301 gcgcctgcag tgcggcgtcg aaggacaggc tcctcgcctc catgccgtcg gccaccgcga
      361 gcagcgccgg cacatcgccc gcggcgagcg cgtcgagcac cgcgtagagg tggtcgtccc
      421 ccaccgtgcc gagcatgtgg ctgacctgct cctcttccac ccggcccgcg ccatgcgcga
      481 tcgcctggtc gagcagcgac agcgcgtcgc gcatcgagcc cgccgccgcc ttggcgaggt
      541 gacgcagcgc gcccggctcg aacgccacct cctcggcctg caggatgcgc gtcaggtgat
      601 cgacgatatg gccttgcggc atctgcttga ggttgaactg caggcagcgc gacagcaccg
      661 tgaccgggat cttctggggg tcggtggtgg cgaggatgaa tttcacatgc tccggcggct
      721 cctcgagcgt cttcaacatg gcgttgaagg cgtgcccggt gagcatgtgc acttcgtcga
      781 tcatgtagac cttgaagcgc ccctgcaccg gcgcatacac cgccttgtcg agcagcgcgg
      841 ccatctcctc gacgccgcgg ttcgaagccg cgtccatctc gacgtagtcg gggaagcggt
      901 cggcatcgat cgcgcggcag gcgtcgcaaa tgccgcaggg ctcggcggtg atgccggtct
      961 cacagttgag tgccttggcg aggatgcggc tgatggtggt cttgcccacg ccgcgcgtgc
     1021 cggtgaacag ccaggcgtgg tgcagccgcc cggtggcgag cgcgtgcgtc agcgcacgca
     1081 cgacgtgctc ctgaccgacc agcgtgccga aggatttcgg ccgccacttg cgtgcgagga
     1141 cctgatagct catggcgggc gattctagca gagcggcggg cggcggcccg cgcgctcgac
     1201 gggacgcagg ttcagccccc cggcgtccgg ctcagcatgc cgcgctcgcc cagccaggca
     1261 agcaccgcat cggccgcggc ctcgaggtcg aggcgggtgg tatcgaggcg cagttcgggg
     1321 tcctccggcg cctcgtaagg cgaatcgatg ccggtgaagt tcttcagctc gccgcgccgg
     1381 gccttcttgt acaggccctt gggatcgcgc gcctcggcca cctccagcgg agtgtcgacg
     1441 aagacctcga cgaactcgcc ctcctccacc aggccgcgcg ccatgcggcg ctcggagcgg
     1501 aaaggcgaga tgaaggcggt gagcacgatc aggcctgcat cgaccatcag cttcgcgacc
     1561 tcggcgacgc gacggatgtt ctccacgcga tcggcgtcgg tgaagccgag gtccttgttg
     1621 aggccatggc gcacattgtc gccgtcgagc aggtaggtgt ggtggccgag cgcgtgcagt
     1681 ttcttctcga ccaggttggc gatcgaggac ttccctgcgc cggacaggcc ggtgaaccac
     1741 agcacgcagc cgcgctggtt cttcagcgcg gcacgcgcgc gcttgtccac atcgacatgc
     1801 tgcaggtgga tgttgtgcgc gcgacgcagc gcgaagtgca acaggccggc gccgacggtg
     1861 ttgttggaga ggcggtcgat caggatgaag ccgccggtgt cgcggttgat ccggtaggga
     1921 tcgaaggcga tcggccgatc caggctcagg ttgcacacgc cgatcgcgtt gagctcgagc
     1981 cgcttggcgg cgacgtgctc gagggtgttc acattcacct ggtacttgat ctcggtgatc
     2041 gtcgcgctga ccgtgcgcgc gccgatcttg agcaggtaag ggcggccggc gagcatgggc
     2101 tcgtcgtgca tccacaccac cgtgcactcg aactggtcgg cgacctcggc cggcgcctcg
     2161 acggtcgaga tgacgtcgcc gcgcgagatg tcgatctcgt cggcgagggt cagcgtgacc
     2221 gactgcccgg ccacggcccg gtcgagatcg ccgtcgcggg tcacgatgcg cgccaccgtg
     2281 ctctcgcgcc ccgagggctg tacgcgcaca cgatcgcccg ggcggatggt gccgccggcg
     2341 atggagccgg caaagccgcg gaagtcgaga ttggggcgat tgacccactg caccggcagg
     2401 cggaagggcg cgcgctgcat caggccgtca tcgacctcca ccgtctcgag gtagcccatc
     2461 agcgtggtgc cgtggtacca gggcatcgca gcgctcggag aggtgatgtt gtcgcccctg
     2521 aaggccgaca tcgggatgaa ggtcacctgc tcgatgccga gctggcggga gaaggcggtg
     2581 tagtcctcga cgatgcggcg gaacacgccc tcggcgtaat cgaccaggtc catcttgttg
     2641 atcgccacca cgatgtggcg gatgccgatg aggttcacca ggtagctgtg acgccgcgtc
     2701 tgggtgagga tgcccttgcg cgcatcgacc atcaggatgg ccgcgtcggc cgtcgaggcg
     2761 ccggtgacca tgttgcgggt gtattgctcg tggccggggg tgtcggcgac gatgaacttg
     2821 cgcttgtcgg tggagaagaa gcggtaggcc acgtcgatcg tgatgccctg ctcgcgctcg
     2881 gc
//
LOCUS       DAOQNN010000406         2879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_25377_length_2879_cov_8.690864, whole genome shotgun sequence.
ACCESSION   DAOQNN010000406 DAOQNN010000000
VERSION     DAOQNN010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25377_length_2879_cov_8.690864"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..564)
                     /locus_tag="PKV97_15295"
     CDS             complement(124..564)
                     /locus_tag="PKV97_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNB07298.1"
                     /translation="MNTHPAFQQFAHSTRNGFEQFQAYALIALDASEKLCALNFGAAR
                     ALCEAASAAPIAQPGSDLEAGFAKQRETQGQVLEQLGNYLQQVNEVVSGAQNELLELG
                     SRHFEDFQASMQSMLEQARKLGGVEVVQSEAAEEPRSRAIRKAA"
     gene            complement(700..1362)
                     /locus_tag="PKV97_15300"
     CDS             complement(700..1362)
                     /locus_tag="PKV97_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB07299.1"
                     /translation="MSLRILLADDHQMFRHALRALLARDPELEVVAEAASGEEVLAIA
                     DAQPVDLVCMDIAMPGMDGIEATRRLLARHPALRVIGLSAFADRQFVTDLLAAGARGY
                     ITKGEAGDELLRAIRTVCEGRSYLCPEVAATVADALRDSPGVYAAAPRLTARERQVLQ
                     LIAEGLTSVQIGDRLHIAASTVEVHRRNLMRKLDLHNVAELTRYAIHSGISPNVTPGG
                     AG"
     gene            complement(1359..2483)
                     /locus_tag="PKV97_15305"
     CDS             complement(1359..2483)
                     /locus_tag="PKV97_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB07300.1"
                     /translation="MRSPRLEALRSALEKAEFRLAATQDLARVGDWELDRHTGRMYWS
                     RELFRLFERPEALGVPDLNEALGYFSLESTNRTRDLFWEAIDSGRRCALEQEVLLPSG
                     EERRHFTVIVPVADETGRVFRLYGTVQDITERRRLEAERLEHLERLEELSRHLVEIEE
                     RERRELASALHDRASPNLAALQILFSSLADALPESARDELAPLLEDASALLADTTAGI
                     REICTNLRPATLDYAGLVPALREYVAQFRARTGLDVRVDAASGSPPCALSRATQTLCF
                     RLVQEALTNCAKHARAGSVRIGLGGCAGGVLLQIGDDGVGFDLSRLGEAGSTPGLGLI
                     TMRERVELAGGDFRLYTRPGDGTVIEVRLPAELHPAETNR"
     gene            complement(2480..>2879)
                     /locus_tag="PKV97_15310"
     CDS             complement(2480..>2879)
                     /locus_tag="PKV97_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB07301.1"
                     /translation="RPQDHAENLQRLEAMIPGFGAGLGAGFDPDRLDGRVGFRPASPD
                     RLPMVGALPLPDRPPASAARGLDELPRRPGLYALSGYGARGLVWSVLAAELLASALEG
                     DPAPLERDLVEAIDPARFVLRPLARTAVRE"
BASE COUNT          393 a          953 c         1120 g          413 t
ORIGIN      
        1 tgcgtaggag cgacgccagt cgcgattgca gcggtgaaag cgggcgcggg cgcgtgtccg
       61 tgcgctgctc ccgcaaaaac aaaccgccgc gagtgcggcg gtcgggatgg cggtgcaggg
      121 ggctcaggcg gccttgcgga tggcgcggct gcgcggctct tcggcggctt cggactggac
      181 gacctcgacg ccgcccagct tgcgcgcctg ttccagcatc gactgcatcg aggcctggaa
      241 gtcttcgaag tggcggctgc cgagttcgag cagctcgttt tgcgcgcccg acaccacctc
      301 gttcacctgc tgcaggtagt tccccagttg ctcgagcacc tgaccctggg tctcgcgctg
      361 cttcgcgaag ccggcctcga ggtcgctgcc cggttgggcg atcggcgcgg ccgaggcggc
      421 ctcgcacagc gcgcgcgcgg cgccgaagtt gagcgcgcac agcttctcgg aggcgtcgag
      481 cgcgatcagg gcataggcct ggaactgctc gaagccgttg cgggtcgagt gggcgaactg
      541 ctggaaggcc ggatgggtgt tcatggcgaa gtctccgcta aaggatgccg ggtggttctc
      601 tcagccagcg atgttaagcg gcaccccgtt ccgggcatat acagcgttcg cagtgtttgg
      661 actaccggtt taccggtagt gcgagggcgc cgggcgcgct cagcccgcgc cgcccggggt
      721 cacgttcggg ctgatgccgc tgtggatcgc gtagcgggtg agttcggcaa cgttgtgcag
      781 gtcgagcttg cgcatcaggt tgcggcggtg cacctcgacc gtggaggcgg cgatgtgcag
      841 gcgatcgccg atctgcaccg aggtcaggcc ttcggcgatc agctgcagca cctggcgctc
      901 gcgcgcggtg agccggggtg cggcggcgta caccccgggg ctgtcgcgca gtgcatccgc
      961 gaccgtcgcc gcgacctcgg ggcacaggta gctgcgcccc tcgcacacgg tgcggatcgc
     1021 gcgcagcagc tcgtcgccgg cttcgccctt ggtgatgtag ccgcgcgcgc cggcggcgag
     1081 caggtcggtc acgaactggc ggtcggcgaa ggccgaaagg ccgatcacgc gcagcgccgg
     1141 atggcgggcg agcaggcggc gggtggcctc gatgccgtcc atgcccggca tggcgatgtc
     1201 catgcacacg aggtcgaccg gctgggcatc ggcgatcgcg agcacctcct cgccactcgc
     1261 cgcctcggcg accacttcga gctccgggtc gcgggcgagc agcgcccgca gcgcgtggcg
     1321 gaacatctgg tggtcgtcgg caagcaggat gcgcaggctc atcggttcgt ttccgcgggg
     1381 tggagctcgg cgggcagccg tacctcgatg acggtgccgt cgccggggcg ggtatacagt
     1441 cggaagtccc cgccggcgag ctcgacgcgc tcgcgcatcg tgatcaggcc cagccccggg
     1501 gtgctgcccg cttcgccgag acgggagagg tcgaagccga cgccgtcgtc gccgatctgc
     1561 agcaggaccc cgccggcgca gccgccgagc ccgatgcgca cgctgccggc gcgagcgtgc
     1621 ttggcgcagt tggtgagcgc ctcctgcact aggcggaagc agagcgtctg cgtcgcgcgg
     1681 gagagggcgc acggggggct gccggacgca gcgtcgacgc gcacgtccag cccggtgcgg
     1741 gcgcggaact gggcgacgta ttcgcgcagt gcgggtacca ggccggcgta gtcgagcgtg
     1801 gccgggcgca ggttggtgca gatctcgcgg atgccggcgg tggtgtcggc gagcagggcc
     1861 gaggcgtcct ccagcagcgg ggcgagctca tcgcgggcgg attcggggag ggcgtcggcc
     1921 aggctggaga acaggatctg cagcgcggcg aggttggggc tggcgcggtc gtgcagcgcg
     1981 ctggcgagtt cgcggcgctc gcgctcctcg atctcgacca ggtggcggga gagctcttcg
     2041 aggcgttcca gatgctccag gcgctcggcc tccaggcgcc ggcgttcggt gatgtcctgc
     2101 accgtaccgt acaggcggaa cacgcgcccg gtctcgtcgg cgaccggcac gatcacggtg
     2161 aaatggcgac gctcctcgcc cgagggcagc aggacctcct gctccagggc gcagcgtcgg
     2221 ccgctgtcga tcgcctccca gaacaggtcg cgggtgcgat tcgtcgactc caggctgaag
     2281 tagccgagcg cctcgttgag gtcgggcacg ccgagcgctt ccgggcgctc gaagaggcgg
     2341 aagagttcgc gcgaccagta catccgcccg gtgtggcggt cgagctccca gtcgccgaca
     2401 cgggcgaggt cctgggtggc ggcgaggcgg aactccgcct tctcgagcgc gctgcgcaag
     2461 gcttcgagcc ggggcgacct cattcgcgca ccgccgtcct ggcaagcggg cgcagcacga
     2521 agcgcgccgg atcgatcgcc tcgaccaggt cgcgctcgag cggggcgggg tcgccttcga
     2581 gcgcgctggc gagcagctcg gcggcgagca ccgaccacac caggccgcgc gcgccgtagc
     2641 ccgagagcgc gtacagcccg ggccggcgcg gcagctcgtc gaggccgcgg gcggccgagg
     2701 cgggaggccg gtcgggcagc gggagggccc cgaccatcgg caggcggtcg ggtgaggcag
     2761 gccggaaccc cacccggcca tcgagccgat cggggtcgaa gcccgcgccc aggccggcgc
     2821 cgaagccggg aatcatggct tccaggcgct gcaggttctc ggcatggtcc tgcgggcgc
//
LOCUS       DAOQNN010000407         2879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_25381_length_2879_cov_5.654037, whole genome shotgun sequence.
ACCESSION   DAOQNN010000407 DAOQNN010000000
VERSION     DAOQNN010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25381_length_2879_cov_5.654037"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="PKV97_15315"
     CDS             complement(<1..1662)
                     /locus_tag="PKV97_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNB07302.1"
                     /translation="MQVQPQHPPALLARYVARPDRYDELCQRIGSVVVLRPHWRDFFH
                     GLAAMPADELSARRAALGRQIHENGITYNVYADPRGFARPWELDLLPYMLPAAEWAQI
                     EAAVIQRATLFDRILADLYGEQTLLEQGLVPPALIYGHSGFLRPLVGARPPGGRFLHL
                     YAVDLARSPDGRWWVVADRTQAPSGAGYALENRSVVARVLPELYRAAGVEPLVPFFEG
                     FRDGLVELAPADGAAAGEDPLIVVLTPGPYNETYFEHAFLAREMGFPLVEGQDLTVRD
                     DKVWLRTLEGLRRVHVILRRVDDLWCDPLELREDSALGVAGLVAAVRAGMVTVANALG
                     SGILETGALLGYLPRLCEHLLGSKLRMPSVATWWCGEPAACEYALKHLRELVIKPAYP
                     TLGARPVFCGDLPAAELDALAARIALRPFDFVAQEMVNLSQAPVLADEAGGAGNKAPA
                     RDARALVARNIGLRVFAAAGAEGFRVLPGGLVRVASEADMRIVSMQHGGGSKDAWVLG
                     SPARPRPIPRIVPGQLAPPAGGARVLGLSSRVAENFFWLGRYSERA"
     gene            complement(1669..>2879)
                     /locus_tag="PKV97_15320"
     CDS             complement(1669..>2879)
                     /locus_tag="PKV97_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNB07303.1"
                     /translation="TEKFMLDGRHTGTGGGNHFVLGGATPGDSPFLRRPDLVRSLLSY
                     WHNHPSLSYLFSGLFIGPTSQAPRVDEARHDSVHELEVAFQQMPEPGIQVPPWLIDRL
                     LRNLLIDASGNTHRAEFCIDKLYSPDSATGRLGLLELRAFEMPPHARMSLAQQLLLRA
                     LIARFWQQPYAPGRLRRWGTELHDRFLLPHWVAEDFRDVVGELREFGYPLEFDWFAPH
                     FEFRFPRYGEFAARGVRVELRMALEPWHVMGEEGAPGGTVRYVDSSVERLQVKVSGLN
                     DDRHVLTCNGRAVPLQPTGTVGEFVAGVRYRAWQPPSCLHPTIGVHAPLVFDLVDGWM
                     QRSMGGCVYHVAHPGGRNHESYPVNPYEAEGRRLARFTTNGHTPGRIAPAPAVRNADF
                     PFTLDLRRQG"
BASE COUNT          427 a         1034 c         1072 g          346 t
ORIGIN      
        1 ggcgcgctcg ctgtagcgtc cgagccagaa gaagttctcc gccacccggc tcgatagccc
       61 gagcacgcgc gcccccccgg ccggaggcgc gagctggccc gggacgatgc gcgggatcgg
      121 gcgcggacgc gccggcgagc cgagcaccca ggcgtccttg ctgccgccgc cgtgctgcat
      181 cgacacgata cgcatgtcgg cctccgaggc cacccggacg agcccgcccg gcagcacgcg
      241 aaacccctcc gcccccgccg ccgcgaacac gcgcagcccg atgttgcgcg cgaccagcgc
      301 gcgcgcgtcg cgcgcgggcg ccttgttccc ggcgccgccc gcctcgtccg ccagcaccgg
      361 ggcctgcgag aggttgacca tctcctgcgc gacgaagtcg aagggccgca gcgcgatccg
      421 tgccgccagc gcgtcgagct ctgccgccgg caggtcgccg cagaacaccg gccgcgcacc
      481 cagggtgggg taggccggct tgatcaccag ctcgcgcagg tgcttcagcg cgtattcgca
      541 ggccgccggc tcgccgcacc accaggtcgc caccgagggc atgcgcagct tgctgccgag
      601 caggtgctcg cacagccgcg gcaggtagcc gagcaacgcc ccggtctcga ggatgccgct
      661 gccgagcgcg ttggccaccg tcaccatgcc ggcgcgcacc gccgccacca ggccggcgac
      721 gccgagcgcg gagtcctcgc gcagctcgag cgggtcgcac cacaggtcgt ccacccggcg
      781 caggatcaca tgcacccggc gcaggccctc gagcgtgcgc aaccagacct tgtcgtcgcg
      841 cacggtgaga tcctggccct cgacgagcgg gaagcccatc tcgcgggcga ggaaggcgtg
      901 ctcgaagtag gtctcgttgt agggcccggg cgtgagcacc acgatcaggg gatcttcccc
      961 ggccgccgcg ccgtcggcgg gcgcgagctc gacgaggccg tcgcggaagc cctcgaagaa
     1021 ggggaccagc ggctcgacgc cggcggcgcg gtagagctcg ggcagcacgc gcgcgaccac
     1081 gctgcggttc tccagcgcgt agcccgcacc cgagggcgcc tgggtgcggt cggccaccac
     1141 ccaccagcgc ccgtcgggcg aacgcgccag gtccaccgcg tacaggtgca ggaagcgccc
     1201 gcccggcggc cgcgcgccca cgagcgggcg cagaaagccg ctatggccat agatgagcgc
     1261 gggcggcacc aggccctgct cgagcagggt ctgctcgccg tagaggtcgg cgaggatgcg
     1321 gtcgaacagg gtggcgcgct ggatcaccgc ggcctcgatc tgcgcccact ccgccgcggg
     1381 cagcatgtag ggcagcaggt cgagctccca cgggcgcgcg aagccgcgcg gatcggcata
     1441 cacgttgtag gtgatgccgt tctcgtggat ctgccggccg agcgcggccc gccgcgccga
     1501 cagctcgtcg gcgggcatgg cggcgaggcc atggaagaag tcccgccagt gcggccgcag
     1561 caccacgacg ctgccgatgc gttggcagag ctcgtcgtag cggtcgggcc gcgcgacgta
     1621 gcgggcgagc agcgcgggcg ggtgctgcgg ctggacctgc atggcgggtc agccctggcg
     1681 gcgcagatcg agggtgaagg ggaagtcggc gttgcgcacg gccggtgcgg gcgcgatgcg
     1741 gccgggcgtg tggccgttgg tggtgaagcg ggccaggcgg cggccttcgg cctcgtaggg
     1801 gttcaccggg tagctctcgt ggttgcgccc gcccggatgc gccacgtgat acacgcagcc
     1861 gcccatcgag cgctgcatcc agccatcgac caggtcgaac accagcggcg cgtgcacgcc
     1921 gatggtcggg tgcaggcagg acggcggctg ccaggcacgg tagcgcaccc cggcgacgaa
     1981 ctcgcccacc gtgccggtgg gctgcagcgg cacggcgcgg ccgttgcagg tcagcacgtg
     2041 gcggtcgtcg ttgagcccgg acaccttgac ctgcaggcgc tccaccgagg aatcgacgta
     2101 gcgcaccgtg ccaccgggcg ccccctcctc ccccatcacg tgccagggct cgagcgccat
     2161 gcgcagctcg acccgcacgc cgcgcgcggc gaactcgccg tacctgggga agcggaactc
     2221 gaagtgcggc gcgaaccagt cgaactcgag cgggtagccg aattcgcgca gctcgccgac
     2281 gacgtcgcgg aaatcctccg ccacccagtg cggcagcagg aagcggtcgt gcagctcggt
     2341 accccagcgc cgcagccgac ccggggcata aggctgctgc cagaagcgcg cgatcagcgc
     2401 gcgcagcagc agctgctggg ccaggctcat gcgcgcgtgc ggcggcatct cgaaggcgcg
     2461 cagctcgagc aggcccagcc gcccggtggc gctgtcgggg ctgtagagct tgtcgatgca
     2521 gaactcggcg cggtgggtgt tgccgctggc gtcgatcagc aggttgcgca gcaggcggtc
     2581 gatgagccac ggcggcacct ggatgcccgg ctccggcatc tgctggaagg cgacttcgag
     2641 ctcgtgcacc gagtcgtggc gcgcttcgtc cacccgcggc gcctgcgagg tcgggccgat
     2701 gaagagcccc gagaacaggt aggacaggct ggggtggttg tgccagtagc tcaacaggct
     2761 gcgcaccagg tcgggccgcc gcaggaaggg cgagtcgccc ggcgtggcac cgccgagcac
     2821 gaaatggttg ccaccgccgg tgccggtgtg gcggccgtcg agcatgaact tctcggtcg
//
LOCUS       DAOQNN010000408         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_25409_length_2877_cov_8.726435, whole genome shotgun sequence.
ACCESSION   DAOQNN010000408 DAOQNN010000000
VERSION     DAOQNN010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25409_length_2877_cov_8.726435"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PKV97_15325"
     CDS             complement(<1..889)
                     /locus_tag="PKV97_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HNB07304.1"
                     /translation="MSASIFAAVEMAPRDPILGLNEAFAADTRAEKVNLGVGVYYDDN
                     GKIPLLAAVRTAEKARLEAMPPRGYQPIEGPAAYNNAVQGLLFGKDSQLATSGQTITV
                     EALGGTGALKVGADYLKRLVPGATVYISDPSWENHRALFESAGFPVENYPYYDAASRG
                     VNFAGMKAKLDSLQPGSIIVLHACCHNPTGADLSDAQWDEVVAVCRERGLIPFLDMAY
                     QGFAEGIDADAVAVRALSASGLQFFVSSSFSKSFSLYGERVGALSIVTASKEEAGRVL
                     SQVKRVIRTNYSNPPIHGGA"
     gene            1041..>2877
                     /gene="uvrB"
                     /locus_tag="PKV97_15330"
     CDS             1041..>2877
                     /gene="uvrB"
                     /locus_tag="PKV97_15330"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNB07305.1"
                     /translation="MTTQGNSAGNDAPQQGGTVLGFEGSPFRLHQPFPPAGDQPEAIR
                     LLCEGVEDGLMYQTLLGVTGSGKTYTMANVIARMGRPALVLAPNKTLAAQLYAEFKEF
                     LPENAVEYFVSYYDYYQPEAYVPSRDLFIEKDSSINEHIEQMRLSATKSLMERRDVVI
                     VATVSCIYGIGDPVDYHAMILHLREGERMAHRDLIQRLVAMQYTRADIDFRRGTFRVR
                     GDVIDVFPAENAELAVRIEMFDDEIEHLTLFDPLTGHLRHKLARFTVYPSSHYVTPRA
                     TVLQAIEAIKEELKERVAFFQREGKLVEAQRIEQRTRFDLEMLNEMGFCKGIENYSRH
                     LSGRGPGEPPPTLIDYLPQDALLFIDESHVAIGQVGGMYKGDRSRKENLVGYGFRLPS
                     ALDNRPLKFDEFERLMPQTVFVSATPAKYEEEHQGQVVEQVVRPTGLIDPMVEVRPAM
                     TQVDDLLSEIKKRLVVNERVLVTVLTKRMAEDLTDYLADNGIRVRYLHSDIDTVERVE
                     IIRDLRLGEFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARH
                     LHGTAILYADRVTDSMKAAIGETERRRQKQIAFNEANGIVPKSVTK"
BASE COUNT          491 a          936 c          995 g          455 t
ORIGIN      
        1 tggcgccgcc gtggatcggc gggttggagt agttggtgcg gatcacgcgc ttgacctgcg
       61 acagcacgcg accggcctct tccttgctgg cggtgacgat ggacagcgca ccgacgcgct
      121 cgccgtagag cgagaagctc ttcgagaacg agctcgacac gaagaactgc aggccgctgg
      181 ccgacagcgc gcgcacggcc acggcgtcgg cgtcgatgcc ttcggcgaag ccctggtagg
      241 ccatgtcgag gaaggggatc aggccgcgct cgcggcacac ggcgaccact tcgtcccact
      301 gcgcgtccga caggtcggcg ccggtggggt tgtggcagca ggcgtgcagc acgatgatcg
      361 agccgggctg caggctgtcg agcttggcct tcatgccggc gaagttcacg ccgcggctgg
      421 cggcgtcgta gtaggggtag ttctcgaccg gaaagccggc ggattcgaac agcgcgcggt
      481 ggttctccca gctcgggtcg gagatgtaca cggtggcgcc ggggacgaga cgcttgagat
      541 agtcggcgcc aaccttgagc gcgccggtgc cgccgagggc ctcgacggtg atggtctggc
      601 cgctggtggc gagctgcgag tccttgccga agagcaggcc ctgcaccgcg ttgttgtagg
      661 ccgcggggcc ttcgatcggc tggtagccgc gcggcggcat ggcctcgagg cgggccttct
      721 cggcggtgcg cacggcggcg agcagcggga tcttgccgtt gtcgtcgtag tacacgccga
      781 cgccgaggtt gaccttctcg gcgcgggtgt cggcggcgaa ggcctcgttg aggccaagga
      841 tcgggtcacg gggcgccatc tcgacggcgg cgaagatcga agcggacatg aggactccag
      901 ttgttccggt agggcggcag tgaatgcgcg tggcgggaac ggacttgcgc cgtgccttgt
      961 cacgcgattt gggaaataat cggcgttttc gtccggtgcc gccgtgcggc gatgcaccgg
     1021 aatggagcgg gaattttagc atgacgactc aaggcaatag cgccggaaac gatgcaccgc
     1081 agcaaggcgg gaccgtgctc ggcttcgagg gcagcccctt ccgcctgcac cagccgtttc
     1141 cgcccgccgg cgaccagccc gaggcgatcc gcctgctgtg cgaaggcgtc gaggacggcc
     1201 tgatgtacca gaccctgctc ggcgtgaccg gctcgggcaa gacctacacc atggccaacg
     1261 tcatcgcccg catggggcga ccggccctgg tgctggcgcc caacaagacg ctggccgcgc
     1321 agctctacgc cgagttcaag gagttcctgc ccgagaacgc ggtcgagtac ttcgtcagct
     1381 actacgacta ctaccagccc gaggcctacg tgccctcgcg cgaccttttc atcgagaagg
     1441 attcgtcgat caacgagcac atcgagcaga tgcgcctgtc ggcgaccaag agcctgatgg
     1501 agcgccgcga cgtggtcatc gtggccacgg tgtcgtgcat ctacggcatc ggcgatccgg
     1561 tcgactacca cgcgatgatc ctgcacctgc gcgagggcga gcgcatggcg caccgcgacc
     1621 tcatccagcg cctggtggcg atgcaataca cccgcgccga catcgacttc cgccgcggca
     1681 ccttccgcgt gcgcggcgat gtgatcgacg tcttcccggc ggaaaacgcc gagctcgcgg
     1741 tgcgcatcga gatgttcgac gacgagatcg aacacctgac cctgttcgac ccgctcaccg
     1801 ggcacctcag gcacaagctc gcgcgcttca ccgtctatcc gtccagccac tacgtgaccc
     1861 cgcgcgcgac ggtgctgcag gcgatcgagg cgatcaagga ggagttgaag gagcgcgtcg
     1921 ccttcttcca gcgcgagggc aagctggtgg aggcgcagcg catcgagcag cgcacccgct
     1981 tcgacctcga gatgttgaac gagatgggct tctgcaaggg catcgagaac tactcccggc
     2041 acctgtccgg gcgcggtccg ggcgagccgc cgccgacgct gatcgactac ctgccgcagg
     2101 atgcgctgct cttcatcgac gagtcgcacg tggccatcgg ccaggtcggc ggcatgtaca
     2161 agggcgaccg ctcgcgcaag gagaacctgg tcggctacgg cttccgcctg ccctcggcgc
     2221 tggacaaccg gccgctgaag ttcgacgagt tcgagcgcct gatgccgcag accgtcttcg
     2281 tctccgccac gccggcgaag tacgaggagg agcaccaggg ccaggtggtg gagcaggtgg
     2341 tgcgcccgac cggcctgatc gacccgatgg tcgaggtgcg cccggcgatg acgcaggtgg
     2401 acgacctgct gtccgagatc aagaagcgcc tggtggtgaa cgagcgtgtg ctggtcaccg
     2461 tgctcaccaa gcgcatggcc gaggacctca ccgactatct cgccgacaac ggcatccgcg
     2521 tgcgctacct gcactcggac atcgacaccg tggaacgggt cgagatcatc cgcgacctgc
     2581 gcctgggcga gttcgacgtg ctggtcggca tcaacctgct gcgcgaaggc ctggacatcc
     2641 cggaggtgtc gctggtggcg atcctcgacg cggacaagga aggcttcctg cgctccgagc
     2701 gttcgctgat ccagaccatc ggccgcgccg cgcgtcacct gcacggtacc gccatccttt
     2761 atgccgaccg cgtcaccgac tcgatgaagg ccgccatcgg cgagaccgag cgccggcggc
     2821 agaagcagat cgccttcaac gaggccaacg gcatcgtgcc gaagtcggtg accaagc
//
LOCUS       DAOQNN010000409         2865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_25588_length_2865_cov_6.432028, whole genome shotgun sequence.
ACCESSION   DAOQNN010000409 DAOQNN010000000
VERSION     DAOQNN010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25588_length_2865_cov_6.432028"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKV97_15335"
     CDS             <1..200
                     /locus_tag="PKV97_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNB07306.1"
                     /translation="EKLSDRELQVFLRLARGESVSDIAAVLNLSVKTISTYRTRLLEK
                     LGVASNAELATYALRHGLILD"
     gene            214..645
                     /locus_tag="PKV97_15340"
     CDS             214..645
                     /locus_tag="PKV97_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB07307.1"
                     /translation="MSAPLNSESATVPALRVVLVEDSAMLRGMLCEMLAELDGVGVVA
                     EAEDEQGALAALEAQRPDLAIIDLELRMGSGLGVLRQLQAEPERFGRPRAVVFSNYGH
                     APVRARCEQLGVERFFDKSFQLDELIDFVQGAIPGIPGIPG"
     gene            complement(652..2046)
                     /locus_tag="PKV97_15345"
     CDS             complement(652..2046)
                     /locus_tag="PKV97_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNB07308.1"
                     /translation="MNADPEPLPLPPAPSRLPHPRAGRLIAVLLLSFVLGLAWLLAGQ
                     WQSESSRVALEDLRARSVRLHHLDTLLLQMLDAESSVRGYLLTQNPVYLRPYQDGQAA
                     LESTLAALRADTWADERQHETVDHLALLIDMRWSLLTQAIERGVPPGADQPDGGTGKQ
                     VTDDIRTRILALRTATQAEIDATQDGAFARFGDARTMNRVLGASVLALLLVLVVVLYR
                     QDRLRERMAQLLRSENERLHTEVAHRTEELSSLATYLTNAREAEQARLARELHDELGA
                     LLTAAKLDAAWIARKLPADTVAPMRSRFDRLLDTLNQGIALKRKVVAGLRPPLLSELG
                     LLEALRALPDSGDIGEQGGRLAIELPDALPDVPAQTALALYRIAQEALTNVRRYAQAS
                     SVRLRLGVEGDELVLAVEDDGVGFDPAARGHNRHGLAGMAHRVQMLAGRLEIDSAPGK
                     GTRITARVPFARMH"
     gene            complement(2232..2543)
                     /locus_tag="PKV97_15350"
     CDS             complement(2232..2543)
                     /locus_tag="PKV97_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNB07309.1"
                     /translation="MKHTAATLALSGLLAFTAVGCAVTRDQSTVGEYVDDATVTTRVK
                     AKFAEDPTVSAMSISVETLKGTVQLSGFAKSTAERNRAAEIARTTPGVKSVKNDIVIR
                     P"
BASE COUNT          372 a          971 c         1062 g          460 t
ORIGIN      
        1 acgagaagct gtccgaccgc gagctgcagg tcttcctgcg cctggcgcgc ggcgagtcgg
       61 tgtcggacat tgccgccgtg ctcaacctga gcgtgaagac catcagcacc taccgcaccc
      121 gcctgctcga gaagctcggc gtggccagca atgccgagct ggcgacctac gcgctgcgcc
      181 acgggttgat cctggactga ggagctcgtg tcgatgagcg cgccactcaa ttccgaatct
      241 gccaccgtcc ccgcgctgcg cgtcgtgctc gtggaagact ccgcgatgct gcgcgggatg
      301 ttgtgcgaga tgctcgccga gctcgacggc gtcggcgtcg tggccgaggc cgaggacgag
      361 caaggggcgc ttgccgcgct cgaggcgcag cgcccggatc tcgccatcat cgacctcgag
      421 ctgcgcatgg gcagcggcct tggcgtgctg aggcaactgc aggccgagcc ggagcgcttc
      481 ggccgtccgc gcgcggtggt gttctccaac tacggccacg cgccggtgcg cgcgcgctgc
      541 gagcagctcg gcgtcgagcg cttcttcgac aaatccttcc agctcgacga gctgatcgac
      601 ttcgtccagg gggccattcc aggcattccc ggcattccgg gctgagccgc ttcagtgcat
      661 gcgtgcgaac ggcacgcgtg cggtgatgcg cgtgcccttg cccggcgcgc tgtcgatctc
      721 cagccgaccg gcgagcatct gcacccggtg cgccatgccg gccagcccgt ggcggttgtg
      781 gccgcgcgcc gccgggtcga agcccacgcc gtcgtcttcc accgccagca ccagctcgtc
      841 gccttcgacc cccaggcgca ggcgcaccga gctcgcctgt gcatagcggc gcacgttggt
      901 cagggcctcc tgcgcgatgc ggtagagcgc aagtgccgtc tgcgcgggca catcgggcag
      961 ggcatcgggc agctcgatcg cgagccggcc gccctgttcg ccgatgtcgc cgctgtcggg
     1021 cagggcgcgc agcgcttcga gcaggccgag ctcggacagc agcggcggac gcaggccggc
     1081 caccaccttg cgcttgagcg cgatgccctg gttcaaggtg tcgagcaggc ggtcgaagcg
     1141 gctgcgcatc ggcgccacgg tgtcggccgg aagcttgcgc gcgatccagg cggcgtcgag
     1201 cttggcggcg gtgagcaagg cgccgagttc gtcgtgcagc tcgcgcgcga ggcgcgcctg
     1261 ctcggcctcg cgcgcattgg tcaggtaggt cgccaggctg ctcagttcct cggtgcggtg
     1321 ggcgacttcg gtgtgcaggc gctcgttctc ggagcgcagc aactgcgcca tgcgttcgcg
     1381 caggcggtcc tggcggtaga gcaccacgac cagcaccagc agcagcgcca gcacgctcgc
     1441 gccgagcacc cggttcatcg tgcgcgcgtc gccaaagcgt gcgaaggcgc catcctgggt
     1501 ggcgtcgatc tcggcctggg ttgcggtacg cagggcgagg atgcgggtgc ggatgtcgtc
     1561 ggtgacctgc ttgccggtgc cgccgtcggg ctggtcggcg ccgggcggaa ccccgcgttc
     1621 gatcgcctgc gtgagcaggc tccagcgcat gtcgatcagc agcgccaggt ggtcgacggt
     1681 ctcgtgctgg cgctcgtccg cccaggtgtc ggcgcgcaag gctgcgagcg tgctttccag
     1741 cgcggcctgg ccgtcctggt aaggcctcag gtagaccggg ttctgcgtca gcaggtagcc
     1801 gcgcacgctc gactcggcgt cgagcatctg cagcagcagg gtgtcgaggt gatgcaggcg
     1861 caccgagcgc gcgcgcagat cctccagcgc gacgcggctc gactcggact gccactggcc
     1921 ggcgagcagc cacgccagcc cgagcacgaa gctcagcagc agcacggcga tcaggcgccc
     1981 cgcgcgcggg tgcggcaggc gggatggggc ggggggcaag ggcagaggct ccggatcggc
     2041 attcatgaac ctgtcctatc ctggcccacg cctgcgggct tgtccgcagc atggggcggc
     2101 agcgcgcacg gccccgggcg cgggctagcg tggaaacggg gctgctgtgc ggtcgggttc
     2161 ccgatggcgg gcaagcccgc cccacgggaa cctccagccc ccgcttcggg cgggggctgc
     2221 ggcgcgcgtc ctcaaggacg gatcacgatg tcgttcttca ccgacttcac gcccggggtg
     2281 gtacgcgcga tctcggcagc gcggttgcgc tcggcggtgc tcttggcgaa gccggacaac
     2341 tgcaccgtgc ccttgagcgt ctcgacgctg atcgacatcg cgctgacggt cgggtcttcg
     2401 gcgaacttgg ccttcacgcg cgtggtcacg gtggcgtcgt cgacgtattc gccgaccgtg
     2461 gattggtcgc gggtgacggc gcagccgacg gcggtgaagg cgagcaggcc ggacagggcg
     2521 agggtggcgg cggtgtgttt catggcgggt ctcctgaggg ttgtcgtgtc ttggcgggtc
     2581 ggtcccgcga gtgggttgca actccactat aggcgcgact tcctgcgggc gttgtccgac
     2641 gaggcggaaa accgtgtagg acgattccgt caccttcccg tcgggggatg ccgatcatcg
     2701 cgtgccgggg cgctcgcccg cgtcgcgcgg gagcctcctg cgcgtgcgca ggccgccgtc
     2761 ctgcgcaggg cggaccgccc tggacggtcc gcgtccgccc tcagggccgc tgcgtctgcg
     2821 cgatcatctt gtcgagcgcc atcgtgtggt ccgcctcctc ggtgg
//
LOCUS       DAOQNN010000410         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_25641_length_2861_cov_10.940485, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000410 DAOQNN010000000
VERSION     DAOQNN010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25641_length_2861_cov_10.940485"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PKV97_15355"
     CDS             complement(<1..760)
                     /locus_tag="PKV97_15355"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNB07310.1"
                     /translation="MPLAIPETPPCVLCLSAGDATAGGGLAADILTLASMGCHPLPVQ
                     TAALVRDTRGIDELWPVAPELIAMQARAVLEDVPVAAFKLGFCGSVENVAVIAEILAD
                     YPDVPLVVEPGLHAAALLGEAGEEIAAALADLILPQTTLLVADRHELLRLTGLAGDEA
                     AESDEEDGGGGGQGETGGAELDEALERLLGNGTEYVLLTGGGDHGPQLVDTLFGSEGI
                     VRTDAGPRPAAPCLGAGTTLAAAAVAAMAHGMALP"
     gene            879..1031
                     /locus_tag="PKV97_15360"
     CDS             879..1031
                     /locus_tag="PKV97_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNB07311.1"
                     /translation="MCLICGFIYDEASGLPDEGIAPGTRWEDVPPNWTCPECNARKED
                     FEMVEI"
     gene            1072..1473
                     /locus_tag="PKV97_15365"
     CDS             1072..1473
                     /locus_tag="PKV97_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB07312.1"
                     /translation="MDLAGLKVMVIDDSNTIRRSAEIFLAQAGCEVVLAEDGFDALAK
                     IADHHPDVIFVDIMMPRLDGYQTCALIKKNPRLSPTPVIMLSSRDGLFDRARGRMAGS
                     DEYLTKPFTKDSLLRAVASHARRGGDAARAG"
     gene            1493..1858
                     /locus_tag="PKV97_15370"
     CDS             1493..1858
                     /locus_tag="PKV97_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB07313.1"
                     /translation="MPIRKILVVDDSPVERLALQEVLGRNGYQVLTAENGEQAIARSR
                     SDHPDLILMDVVMPGMNGYQATRTISRDDETRDIPIIMCTSKGQETDKIWGMRQGALD
                     YLVKPVDHDELLARIRAMG"
     gene            1864..2370
                     /locus_tag="PKV97_15375"
     CDS             1864..2370
                     /locus_tag="PKV97_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNB07314.1"
                     /translation="MARSSLREFQERLARRLAEAAGAERRGLLGFQCGSDNWLIELAE
                     AGEILPPPPLSAVPLTRPWFRGLANVRGSLYGVVDFAAFNGQPPISAGGRARLLLIGA
                     RHGIHSALLVSSSSGLRSHEDFEPAPGGDAPPWVARRLRDMQGRAWLQLDPARLLAQP
                     AFLDAGLE"
     gene            2420..>2861
                     /locus_tag="PKV97_15380"
     CDS             2420..>2861
                     /locus_tag="PKV97_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB07315.1"
                     /translation="MALNPFGKKAPAPGAGTEAQTIIETSAPEQTVANKTPSPPARSG
                     GRLGMLGGLFVACTAISAGLLAYQARDASLSALQLGAASELQVLPGQMARAARLARLG
                     EPGAVADLEQTRARFADLLQTLGQGGGHAGATLPPLPASMRPLLQ"
BASE COUNT          443 a         1082 c          962 g          374 t
ORIGIN      
        1 cgggcagcgc catgccgtgc gccatcgctg ccaccgcggc ggcggcgagg gtggtgccgg
       61 cacccaggca gggcgcggcc gggcgcgggc cggcgtcggt gcgcacgatg ccctcgctgc
      121 cgaagagggt gtcgacgagc tgcgggccgt ggtcgccgcc gccggtgagc agcacgtatt
      181 cggtaccgtt gccgagcagg cgctcgagcg cctcgtcgag ctcggcgcca cccgtctcgc
      241 cctggcctcc cccgccaccg tcctcctcgt cggactcggc cgcctcgtcc ccggccaggc
      301 cggtcaggcg cagcagctcg tggcggtcgg ccaccaacag cgtggtctgc ggcaggatca
      361 ggtcggcgag cgccgcggcg atctcctcgc ccgcctcgcc gagcagcgcc gcggcgtgca
      421 ggccaggttc gaccaccagg ggaacgtcgg gatagtcggc gaggatctcc gcgatcaccg
      481 cgacgttctc gacgctgccg cagaagccga gcttgaacgc cgccaccggc acgtcctcga
      541 gcaccgcgcg cgcctgcatg gcgatcagct cgggcgcgac cggccacagc tcgtcgatgc
      601 cgcgggtgtc gcgcaccagc gccgcggtct gcaccggcag cgggtggcag cccatgctcg
      661 ccagggtcag gatgtcggcc gcgagtcctc cgccggccgt ggcgtcaccc gccgacaggc
      721 acagcacgca ggggggagtt tccggaatgg cgagcggcat gggaggtgga aatgcagcgc
      781 ggatggggtg cgcgcggatg cgttaagata ggcggtcgat tctaccccca accctcgagt
      841 cacgctggaa catggccgat accccctgga agacctacat gtgcctgatc tgcggcttca
      901 tctacgacga agcgtcgggc ctgcccgacg agggcatcgc gccgggcacg cgctgggagg
      961 acgttccgcc gaactggacc tgtcccgagt gcaacgcgcg caaggaagac ttcgagatgg
     1021 tcgagatcta ggcccggacg ccaccgcccg acaacccacc aggaatacag catggacctt
     1081 gccggcctga aggtgatggt catcgacgac agcaacacga tccgccgcag cgccgagatc
     1141 ttcctcgccc aggcgggctg cgaggtcgtc ctcgccgagg acggtttcga cgcactcgcg
     1201 aagatcgccg accaccaccc cgacgtcatc ttcgtggaca tcatgatgcc gcgcctcgat
     1261 ggctaccaga cctgtgcgct gatcaagaag aacccccgcc tgtcgcccac gccggtgatc
     1321 atgctgtcct cgcgcgacgg cctcttcgac cgcgcgcgcg gacgcatggc gggctccgac
     1381 gaatacctca ccaaaccctt caccaaggac agcctgttgc gggcggtcgc cagccacgca
     1441 cggcgcggcg gcgatgcggc gcgcgcaggc tgagccacgc cggagacgac cgatgccgat
     1501 ccgcaagatt ctcgtggtcg acgactctcc cgtcgaacgc ctcgccctcc aggaggtgct
     1561 gggccgcaac ggctaccagg tgctgaccgc cgagaacggc gagcaggcca tcgcacgcag
     1621 ccgcagcgac caccccgacc tcatcctgat ggacgtggtg atgccgggca tgaacggcta
     1681 ccaggccacg cgcacgatct cgcgcgatga cgagacgcgc gacatcccga tcatcatgtg
     1741 caccagcaag ggccaggaga ccgacaagat ctggggcatg cgccagggcg cgctcgacta
     1801 cctggtcaag ccggtcgacc acgacgaact gctcgcccgc atccgggcga tgggctgagc
     1861 tcgatggccc gcagcagcct gcgcgaattc caggaacgcc tcgcccgtcg cctcgccgag
     1921 gccgccggcg ccgagcgccg cggcctgctc ggcttccagt gcggcagcga caactggttg
     1981 atcgagctcg ccgaggccgg cgagatcctg cccccgccgc ccttgtccgc ggtgccgctc
     2041 acccgcccct ggttccgcgg cctcgccaac gtgcgcggca gcctgtacgg cgtggtcgac
     2101 ttcgccgcct tcaacggcca gccgccgatc agcgccggcg ggcgggcgcg cctgctgctg
     2161 atcggtgcgc gccacggcat ccacagcgcg ctgctggtgt cgtcctcgag cggcctgcgc
     2221 agccatgagg acttcgagcc cgccccgggc ggcgatgccc cgccctgggt ggcacgccgc
     2281 ctgcgcgaca tgcagggccg cgcgtggctg cagctcgacc cagcccgcct gctcgcccag
     2341 cccgccttcc tcgacgccgg gctggaatga cgtacgcatc cactccgcaa gaacccgacc
     2401 gaaaaatcga gcatcgacca tggcattgaa cccgttcggc aagaaagccc cggcccccgg
     2461 cgccggcacc gaggcccaga cgatcatcga gacctccgcg ccggagcaga cggtcgccaa
     2521 caagactccg tctccccccg ctcgatccgg cggccgcctc ggcatgctcg gcggactctt
     2581 cgtcgcctgc acggcgatca gcgccggact gctcgcatac caggcgcggg atgcgagcct
     2641 gtccgcgctc cagctcggcg cggcgagcga actgcaggtg ctgcccggac agatggccag
     2701 ggcggcccgc ctcgcccgcc tcggcgagcc cggcgcggtc gccgatctcg agcagacccg
     2761 ggcacgcttc gccgacttgc tgcagaccct cggccagggc ggcggccatg ccggcgccac
     2821 gctgccgccg ctgcccgcga gcatgcggcc cctgctgcag g
//
LOCUS       DAOQNN010000411         2855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_25720_length_2855_cov_12.659286, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000411 DAOQNN010000000
VERSION     DAOQNN010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25720_length_2855_cov_12.659286"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PKV97_15385"
     CDS             <1..376
                     /locus_tag="PKV97_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB07316.1"
                     /translation="GIRVVPLVGPSSILLGLMGSGLNGQSFAFHGYLPVAEGERDGAL
                     RTLEDESRKLGRTQIFIETPYRNERMLDALLRTLKPETRLCVACELNTADEFLLTRSV
                     REWKKAARPALARRPALFLLLA"
     gene            complement(392..1048)
                     /locus_tag="PKV97_15390"
     CDS             complement(392..1048)
                     /locus_tag="PKV97_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNB07317.1"
                     /translation="MAERFDLIVFDWDGTLMDSAAAIVRAMQAAARDLDLPVPSEERA
                     RYVIGLGLGDALRHAVPDLEESDYPRMVERYRHHYLSSDHELSLFEGVDALIDALAGR
                     GHLLAVATGKSRVGLNRALGHTGLGRYFHATRCADECFSKPHPAMLEALMDELGVAPE
                     RTLMIGDTTHDLQMAKNARTAGLAVGFGAHPVEVLVAESPLACVRTPGELAGWLLANA
                     "
     gene            complement(1041..2000)
                     /locus_tag="PKV97_15395"
     CDS             complement(1041..2000)
                     /locus_tag="PKV97_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB07318.1"
                     /translation="MTTIEGKAIAVRHERIDEAAAGQRIDNYLLRIAKGVPKSHVYRI
                     LRSGEVRVNGRRVQQTYRLQEGDEVRIPPIRIAEPTHAAPAPAGKPLPVVYEDEALLV
                     IDKPSGKAVHGGSGVSYGVIEQLRVQRPELRMLELAHRLDRETSGLLIVAKKRSALTA
                     LHDMMREGSVEKRYLTLVPGRWSNPLQHVKVPLHKYLTAEGERRVRVADEGKAAHSIV
                     RLVRRWRDYSLLEVELKTGRTHQIRVHLAHLGFALCGDDKYGDFALNKRLEVEGLRRM
                     FLHAAKLRFAHPLTGAPLAFDSPLPADLQTFLDRLDAAEKKHG"
     gene            2452..>2855
                     /locus_tag="PKV97_15400"
     CDS             2452..>2855
                     /locus_tag="PKV97_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNB07319.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQKLIDLDIESAAKEERKSNIYKA
                     VITRIEPSLEAAFVDYGADRHGFLPFKEISRSYFQPGADAKASIKEALKEGQELIVQV
                     EKDERGNKGAALTTYISLAGRYLVLMPNNPRGG"
BASE COUNT          492 a          999 c          922 g          442 t
ORIGIN      
        1 cggcatccgc gtcgtaccgc tggtcggccc ctcctccatc ctgctcgggc tgatgggctc
       61 ggggctcaac ggccagagct tcgccttcca cggctacctg ccggtggccg agggcgagcg
      121 cgatggggcc ctgcgcacgc tcgaggacga atcccggaag ctcgggcgca cgcagatctt
      181 catcgagacg ccttatcgca acgagcgcat gctcgacgcc ctgctgcgca cgctcaagcc
      241 cgaaacccgg ctgtgcgtgg cctgcgaact caacaccgcg gacgaattcc tcctcacccg
      301 cagcgtgcgc gagtggaaga aggctgcgcg ccccgccctc gcccgccgcc ccgcgctctt
      361 cctgctgctg gcctgaacga cgctacgcgg ctcaggcgtt ggccagcagc cagcccgcga
      421 gctcgcccgg cgtacgcacg caggcgagcg gggactcggc caccagcacc tcgaccggat
      481 gcgcgccgaa gcccaccgcc agcccggcgg tgcgcgcgtt cttcgccatc tgcaggtcgt
      541 gggtggtgtc gccgatcatc agggtgcgct ccggcgccac gccgagctcg tccatgagcg
      601 cttcaagcat cgccggatgc ggcttggaga agcactcgtc ggcgcagcgc gtggcatgaa
      661 aatagcgacc cagcccggtg tggccgagcg cacggttcag gcccacgcgg ctcttgccgg
      721 tggcgaccgc cagcaggtga ccgcgcccgg cgagcgcgtc gatcagcgcg tcgacgccct
      781 cgaagaggct cagctcgtga tccgaggaca ggtagtggtg acgatagcgc tcgaccatgc
      841 gcggatagtc ggactcttcc agatcgggca cggcgtggcg caacgcatcg cccaggccga
      901 ggccgatgac gtagcgcgcg cgctcctcgg agggcaccgg cagatcgagg tcgcgcgccg
      961 cggcctgcat cgcacgcacg atggcggcag ccgaatccat caaggtgccg tcccagtcga
     1021 agacgatcag gtcgaaacgt tcagccatgc tttttctccg cggcatcgag ccggtcgagg
     1081 aaggtttgca ggtccgcggg cagcggcgaa tcgaaggcga gcggcgcacc ggtgagcggg
     1141 tgggcgaagc gcagcttcgc ggcgtgcagg aacatccgcc gcaagccctc gacctccagg
     1201 cgcttgttga gcgcgaagtc gccgtatttg tcgtcgccgc acagcgcgaa gccgagatgg
     1261 gcaagatgca cgcggatctg atgcgtgcgc ccggtcttca gttccacctc gagcaggctg
     1321 tagtcgcgcc agcgccgtac caggcgcacg atgctgtgcg ccgccttgcc ctcgtccgcg
     1381 acgcgcaccc ggcgctcgcc ttcggcggtc aggtacttgt gcagcggaac cttgacgtgc
     1441 tgcagcggat tggaccagcg ccccggcacc agggtcagat agcgcttctc cacgctgccc
     1501 tcgcgcatca tgtcgtgcaa cgcggtgagc gccgagcgtt tcttcgcgac gatcagcagc
     1561 cccgaggtct cgcggtcgag gcgatgggcg agctcgagca tgcgtagctc ggggcgctgc
     1621 acgcgcagtt gctcgatcac cccgtagctc accccgctgc cgccatgcac cgccttgccg
     1681 gagggcttgt cgatgacgag cagcgcctcg tcctcgtaca ccaccggcag cggcttgccc
     1741 gccggcgcgg gtgcggcgtg ggtcggctcg gcgatgcgga tcggcgggat gcgcacctcg
     1801 tcgccctcct gcaggcgata ggtctgctgc acgcgacgcc cgttcacccg cacctcgccg
     1861 ctgcgcagga tgcgatagac gtggctcttg ggcaccccct tggcgatgcg caggaggtag
     1921 ttgtcgatgc gctggcccgc cgccgcctcg tcgatgcgtt cgtggcgaac cgcgatcgct
     1981 ttgccttcaa tcgtcgtcat gaaatatact ttcgcgcggt tgaaccggtc gctgccaccc
     2041 aagtgcggcg cgtcagcagt ccgtcaggtc gaaacacctg ctccggacga cgcgcgcaga
     2101 ctgctggcaa agagccctga agagcgctcc cggtcacaac ccttgcccgc catccaaggc
     2161 cgaaaaggcg ccgatggtaa cgcaagcgat ccccttcgac acacccatcc agcccccggg
     2221 ttggattttg acgcgcaagt caccgacgca ggatcaaatg ttccggccac gctgccgcac
     2281 cggcaccgcg ggcgggaaca agagaaattc gaccagccga cccatcacag ccctgttaac
     2341 ccaccggatg aaagcagcag cagccctacc caatcgctcc tgatcccgga aagcgcgatg
     2401 gtgcgcgccg ttcgtcctgc ccgtgtgtct tctcgcggga gaacgatcca aatgaagcgc
     2461 atgcttttca atgcgacgca ggccgaagaa ctgcgcgtcg cgatcgtgga cggtcagaaa
     2521 ctgatcgacc tcgatatcga atcggccgcc aaggaagaac gcaagagcaa catctacaag
     2581 gccgtcatca cccgcatcga gcccagcctc gaggcggcgt tcgtggacta cggtgccgac
     2641 cgccacggct tcctgccgtt caaggagatc tcgcgttcct acttccagcc tggtgcggac
     2701 gccaaggcct cgatcaagga agccctgaag gaaggccagg aactcatcgt ccaggtcgag
     2761 aaggacgagc gcggcaacaa gggtgcggcg ctcaccacct acatctcgct ggccggccgc
     2821 tatctggtgc tgatgcccaa caacccgcgc ggcgg
//
LOCUS       DAOQNN010000412         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_25832_length_2849_cov_8.760558, whole genome shotgun sequence.
ACCESSION   DAOQNN010000412 DAOQNN010000000
VERSION     DAOQNN010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25832_length_2849_cov_8.760558"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1235)
                     /gene="holA"
                     /locus_tag="PKV97_15405"
     CDS             complement(225..1235)
                     /gene="holA"
                     /locus_tag="PKV97_15405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNB07320.1"
                     /translation="MNLRPDQLAAQLERPLAPLWLLHGNEPLLVLEAADAIRAAARRQ
                     GYEERETLVVGHGFKWDALALAAGNLSLFGGAKLIDLRIPTGKPGRDGGDALQRYIAH
                     LPSQTLTLVTLPEVDWATRKTAWFKALADAATTLELHAPERERLPEWVARRLAAQKQS
                     APPEALAFIADHVEGNLLAAHQEILKLGLLHGEGALTLEQVQDAVLDVARYDIDKLRQ
                     AVIEGDPARCARLLEGLEGEGAAPPLVLWALAHETRSLATLCAGRDAGQPLATLFKAE
                     RIFDPRRQQAMTRALGRLGQGKLRAALMHAARIDRMIKGLASGDLWDEFLQLALRLAR
                     RG"
     gene            complement(1255..1782)
                     /gene="lptE"
                     /locus_tag="PKV97_15410"
     CDS             complement(1255..1782)
                     /gene="lptE"
                     /locus_tag="PKV97_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNB07321.1"
                     /translation="MTAPGPVMATARRRLLGGALGAAALLLSGCGFRLRGPQALDFAT
                     VHISVPEQSEFGAQLRRLVATTGTTRVEEDAAKAEARLQILSNDRGREILSLTGAGKV
                     REYQLVQSLRFQLLDRAGKALIPPTSLTARREYTFDDSQVLGKEQEEALLYRDMQNDL
                     VQQLMRRLAAARRSG"
     gene            complement(1794..>2849)
                     /locus_tag="PKV97_15415"
     CDS             complement(1794..>2849)
                     /locus_tag="PKV97_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB07322.1"
                     /translation="NYWAPVDQYIGGIEHAILHLLYSRFFTRAMRDCGLVKVSEPFSA
                     LLTQGMVVAETYYRELDGGKKQWINPADVVVERDERGRIVAAKLESDGLPVVIGGTEK
                     MSKSKNNGVDPQSLVDQYGADTARLFMMFAAPPDQQLEWSDAGVEGASRFLRRVWNYG
                     HAFATATRAALPAERKLAANALPTAAADVRREVHTHLKQASYDFGKHQFNTVVSAAMK
                     ILNALEKAHKDLAAAEPAAHAEVAEEGFSILLRLLSPITPHICHALWQDCGFGTDILA
                     AQWPEVSEDALKRDEIELMLQVNGKLRGSLEVAADAAKADIEALALASEAAQKFMEGK
                     PAKKVVVVPGRLVNIVV"
BASE COUNT          384 a          930 c         1086 g          449 t
ORIGIN      
        1 acttcttgag gaaggccggg tcttgctttt tgagcacacg gatggcggcc cagggtctgc
       61 atgtagtgct ggatgtccat ggcgggctct tcttcggcgt cggtcgcggc gttcgggagc
      121 gccgcggggc aaaccggcat taaagcatcg aaccggacgc ggatgaagcc tggcgcgagc
      181 gtgcagcgag tgcagtgcga ggggtgcgcg agtgggggcg tgattcagcc gcggcgcgcc
      241 aggcgcaggg cgagctggag gaattcgtcc cacaggtcgc cgctggcgag gcccttgatc
      301 atgcggtcga tgcgtgcggc gtgcatcagc gcggcgcgca gcttgccctg gccgaggcgc
      361 ccgagcgcgc gcgtcatggc ctgctggcgg cgcggatcga agatgcgctc ggccttgaac
      421 agcgtcgcca ggggctggcc tgcgtcgcgc ccggcgcaaa gcgtggcgag gctgcgcgtc
      481 tcgtgggcga gcgcccatag caccagcggc ggggcggcgc cctcgccctc gaggccctcg
      541 agcaggcgag cgcagcgcgc cgggtcgccc tcgatgaccg cctgtcgcag cttgtcgatg
      601 tcgtagcgtg cgacgtcgag caccgcgtcc tgcacctgct cgagcgtgag cgcgccctcg
      661 ccgtgcagca ggccgagctt gaggatctcc tggtgggcgg cgagcaggtt gccttcgacg
      721 tgatcggcaa tgaaggcgag cgcctcgggc ggggcggact gcttctgcgc ggcgagccgg
      781 cgcgcgaccc actcgggcag acgttcgcgt tcgggggcgt ggagctccag cgtcgtcgcg
      841 gcgtcggcca gggccttgaa ccaggcggtc ttgcgcgtcg cccaatcgac ttccggcagg
      901 gtgaccaggg tcagggtctg ggaaggcagg tgggcgatgt agcgttgcag cgcatcgccg
      961 ccgtcgcggc cgggcttgcc cgtcgggatg cgcaggtcga tcagcttggc gccgccgaag
     1021 agcgagaggt tgcccgcggc gagggcgagc gcgtcccact tgaagccgtg gccgacgacc
     1081 agggtctcgc gctcctcgta gccctggcga cgggctgcgg cgcggattgc gtcggcagcc
     1141 tcgagcacca gcagcggctc gttgccgtgc agcagccaga gcggcgcgag cgggcgctcg
     1201 agctgggcgg cgagctggtc ggggcgcagg ttcacgcggg gcggcggaac gggttcagcc
     1261 gctgcggcgg gcggcggcga ggcggcgcat gagctgctgc accaggtcgt tctgcatgtc
     1321 gcggtagagc aaggcctctt cctgttcctt gccgagcacc tgactgtcgt cgaaggtgta
     1381 ctcgcgccgc gccgtcaggc tggtgggcgg gatcagcgcc ttgcccgcgc ggtcgaggag
     1441 ctggaagcgc aaggactgga cgagctggta ctcgcgcacc ttgcccgcac cggtgaggct
     1501 gaggatctcg cggccgcggt cgttggagag gatctgcagg cgggcttcgg ccttggctgc
     1561 gtcctcctcg acccgggtgg tgccggtggt ggcgacgagg cggcgcagct gggcgccgaa
     1621 ttcgctctgc tccggcacgc tgatgtgcac ggtcgcaaag tcgagcgcct gcgggccgcg
     1681 caggcgaaag ccacagcccg acagcagcag cgccgcggca ccgagggcgc caccgagcag
     1741 gcgccgccgc gcggtggcca tgacgggacc aggagcagtc atgccgcgct tcctcaaacg
     1801 acgatgttga ccaggcggcc gggcacgacg accaccttct tcgccggctt gccctccatg
     1861 aacttctgcg ccgcttccga agcgagggcg agcgcctcga tgtcggcctt cgccgcgtcg
     1921 gccgcgacct cgaggctgcc gcgcagcttg ccgttgacct gcagcatcag ctcgatctcg
     1981 tcccgcttca gcgcgtcctc gctgacctcg ggccactgcg ccgccaggat gtcggtgccg
     2041 aagccgcagt cctgccacag cgcgtggcag atgtgcgggg tgatcggcga cagcaggcgc
     2101 agcaggatcg agaagccttc ctcggctacc tcggcgtggg ccgccggctc ggcggcggca
     2161 agatccttgt gcgccttctc gagcgcgttg aggatcttca tcgccgccga caccacggtg
     2221 ttgaactggt gcttgccgaa gtcgtagctc gcctgcttga ggtgggtgtg cacctcgcgg
     2281 cgcacgtcgg ctgcggcggt gggcagcgca ttggcggcga gcttgcgctc ggcgggcagc
     2341 gcggcgcggg tggcggtggc gaaggcgtgg ccgtagttcc acacccggcg caggaagcgc
     2401 gaggcgccct cgacgccggc gtcggaccac tccagctgct ggtcgggcgg cgcggcgaac
     2461 atcatgaaca ggcgcgcggt gtcggcgccg tactggtcca ccagcgactg cgggtcgacg
     2521 ccgttgttct tcgacttcga catcttctcg gtgccgccga tgaccaccgg caggccgtcg
     2581 gattcgagtt tcgcggcgac gatgcggccg cgctcgtcac gctcgacgac cacgtcggcc
     2641 gggttgatcc actgcttctt gccgccgtcg agctcgcggt aataggtctc ggccaccacc
     2701 atgccctggg tgagcagcgc gctgaagggc tcggagacct tcaccaggcc gcagtcgcgc
     2761 atcgcgcggg tgaagaagcg cgagtacagc aggtgcagga tggcgtgctc gatgccgccg
     2821 atgtactggt cgaccggcgc ccagtagtt
//
LOCUS       DAOQNN010000413         2847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_25870_length_2847_cov_8.805158, whole genome shotgun sequence.
ACCESSION   DAOQNN010000413 DAOQNN010000000
VERSION     DAOQNN010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25870_length_2847_cov_8.805158"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PKV97_15420"
     CDS             complement(<1..1077)
                     /locus_tag="PKV97_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNB07323.1"
                     /translation="MNPAAPSTAEAPIVPSAPRFVHLRLHSEYSITDGIVQLDQAIAA
                     AAADGMPALGVSDLANLFGMVKFYKGARGKGIKPIVGVDAWIRNEAERDKPQRVLLIC
                     KNRAGYGQLCELLTRAYLENKHRGRAEMRREWFQNGAASALLCLSGAMSGDIGAAIAA
                     GNLALAEQLAADWARLFPDAFYIEIQRAGHPGTESYIRHAVELAGRLGLPVVATHPVQ
                     FLKREDFKAHEARVCIAQGYVLADKRRPRDFTEEQYLKSQAEMCELFADLPEALENAV
                     EIARRCSLTVQLGKNFLPLFPTPEGMTLDDFLVAEAKKGLEERLAQLYPHPEERERQR
                     PRYEQRLKFETDTIIQMGFPGYFLI"
     gene            complement(1121..2095)
                     /locus_tag="PKV97_15425"
     CDS             complement(1121..2095)
                     /locus_tag="PKV97_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HNB07324.1"
                     /translation="MTHAIRIHRTGGPEVLQWEAVELPPPATGEVRLRHHAVGLNFID
                     TYHRSGLYPLTLPSGLGMEGAGVVEAVGDGVAELKVGDRVAYTSGPLDAYAEARNIEA
                     RHLVPLPDDISFEQGAAMMLQGITAQYLLHATYPVKAGETILVHAAAGGVGSILVQWA
                     KQLGATVIGTVGNDDKAQRARALGCDHVIVYSRENFPERVRALTGGEGVAVVYDSVGR
                     DTFLDSVACLRRRGTMVSYGNATGPVAAFDCALLHKAGSVYVTRPGLPDYIASRAELV
                     ERAAALFEVVRSGQVRIEILQRYALKDAAQAHRDLEARKTTGSTVLLP"
     gene            complement(2163..>2847)
                     /locus_tag="PKV97_15430"
     CDS             complement(2163..>2847)
                     /locus_tag="PKV97_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HNB07325.1"
                     /translation="YFLIGGRDPVSARFQFSFRYRIFDEQGVVAETIPVASGLYFGFT
                     QTSLWDLQSESKPFRDSSFRPSLFYRFALDDPQKRGSLALSGGYEHESNGKEDMPSRS
                     IDTLFVRADARLRLDESGSYLGVVPKVWTYLDREDNPDIARYRGHAELGLRLGRDDGW
                     LFSTLIRRGTEGKMGTQYDLSYPIRRSVFSGVGAFVHLQAFKGYGETLLEYDENKEAQ
                     YRIGVSLVR"
BASE COUNT          442 a          955 c         1002 g          448 t
ORIGIN      
        1 gatcaggaag tagccgggga agcccatctg gatgatggtg tcggtctcga acttcagccg
       61 ctgctcgtag cgcgggcgct gacgctcgcg ctcctccggg tgcgggtaga gctgggcgag
      121 gcgctcctcc agccccttct tcgcctccgc gacgaggaag tcgtcgagcg tcatgccctc
      181 gggcgtcggg aacaagggca ggaagttctt gccgagttgc acggtgagcg agcagcggcg
      241 cgcgatctcg accgcgttct cgagcgcctc gggcagatcg gcgaagagct cgcacatctc
      301 cgcctggctc ttgaggtact gctcctcggt gaagtcgcgc ggccggcgct tgtcggcgag
      361 cacgtagccc tgcgcgatgc agacgcgcgc ctcgtgtgcc ttgaaatcct cgcgcttgag
      421 gaactgcacc gggtgggtcg ccaccaccgg caggccgagc cggccggcga gttccaccgc
      481 gtggcggatg taggactcgg tgccgggatg cccggcgcgc tggatctcga tgtagaaggc
      541 gtccggaaac agccgcgccc agtcggccgc gagctgttcg gcgagcgcga ggttgccggc
      601 cgcgatcgcc gcgccgatgt cgccgctcat cgcacccgac aggcacagca gcgcgcttgc
      661 ggcaccattc tggaaccact cacggcgcat ctcggcgcgg ccgcggtgct tgttctcgag
      721 gtaggcgcgc gtcagcagct cgcacagctg accatagccc gcgcggttct tgcagatgag
      781 cagcacgcgc tgcggtttgt cgcgctcggc ctcgttgcgg atccaggcgt cgacgccgac
      841 gatgggcttg atgcccttgc cgcgcgcgcc cttgtagaac ttgaccatgc cgaacaggtt
      901 ggcgagatcg gagacgccga gcgccggcat gccgtcggcc gcggcggcgg cgatcgcctg
      961 gtcgagctgg acgatgccgt cggtgatcga atattcggag tgcaggcgga gatgcacgaa
     1021 gcgcggagcg ctcggcacga tgggcgcttc ggccgtggag ggggcagcag gattcatggc
     1081 gcggatttta cccgacgccg cccgcccccg ccgccgacac tcagggcaac agcaccgtcg
     1141 agccggtggt cttgcgcgcc tcgaggtcgc ggtgcgcctg ggccgcgtcc ttcagcgcat
     1201 agcgctgcag gatctcgatc ctcacctggc cgctgcgcac cacctcaaac agcgccgcgg
     1261 cgcgttcgac gagctccgcg cggctggcga tgtagtcggg cagcccgggc cgagtgacat
     1321 agaccgagcc cgccttgtgc agcaaggcgc aatcgaaggc ggcgaccgga ccggtggcgt
     1381 tgccgtagct caccatggtg ccgcggcggc gcagacaggc gaccgaatcg aggaaggtgt
     1441 cgcgcccgac cgaatcgtac accaccgcga cgccttcgcc gccagtgagc gcgcgcacgc
     1501 gctcggggaa gttctcgcgc gagtacacga tgacgtggtc gcagccgagc gcgcgcgcgc
     1561 gctgcgcctt gtcatcgttg cccacggtgc cgatcacggt tgcgccgagc tgcttcgccc
     1621 actgcaccag gatcgagccg accccacccg ccgccgcatg caccaggatg gtctcgcccg
     1681 ccttgaccgg gtaggtggcg tgcaacaggt attgtgcggt aatgccctgc agcatcattg
     1741 ccgcgccctg ctcgaaggag atgtcgtccg gcaacggcac caggtggcgc gcctcgatgt
     1801 tgcgcgcctc ggcataggca tcgagcgggc cgctggtata ggccacgcgg tcgcccacct
     1861 tcagctcggc gacaccgtcg ccgaccgcct ccaccacgcc cgccccctcc atgcccagcc
     1921 cggacggcag cgtcagcggg tacaggccac tgcggtgata ggtgtcgatg aagttgagcc
     1981 ccaccgcatg gtggcgcagg cgcacctcgc cggtcgcggg tggcggcagc tcgacggcct
     2041 cccactgcag gacttcgggg ccgccggtgc ggtggattcg gatcgcatgg gtcatcggat
     2101 tctcctcggg attccttgcg cgcagcgtcg gagggagccc ggccacgcgg cgtcgggtga
     2161 tcttagcgta ccagcgagac cccgatgcgg tactgcgctt ccttgttctc gtcgtactcg
     2221 agcagggtct cgccatagcc cttgaaggcc tgcagatgga cgaaggcgcc caccccggag
     2281 aacacgctgc gccggatcgg ataggacagg tcgtactggg tgcccatctt gccctcggtg
     2341 ccgcgccgga tcagcgtgga gaacagccag ccgtcgtcgc ggccgaggcg caggccgagc
     2401 tcggcgtggc cgcggtagcg cgcgatgtcg ggattgtcct cccgatcgag gtaggtccac
     2461 accttgggca cgacgccgag gtaggagccg gactcgtcca ggcgcaggcg cgcgtcggcg
     2521 cgcacgaaga gcgtgtcgat gctgcgcgag ggcatgtcct ccttgccgtt cgattcgtgc
     2581 tcgtaaccgc cggagagtgc cagcgaaccg cgcttttgcg ggtcgtcgag cgcgaagcgg
     2641 tagaacagcg aagggcggaa gctcgaatcg cggaatggct tggattccga ctgcaggtcc
     2701 cacagcgagg tctgggtaaa gccgaagtag agcccgctcg ccaccgggat cgtctccgcc
     2761 accacgccct gctcgtcgaa gatgcggtaa cggaagctga actggaagcg cgccgacacc
     2821 gggtcgcgtc cgccgatgag gaagtac
//
LOCUS       DAOQNN010000414         2843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_25926_length_2843_cov_9.167862, whole genome shotgun sequence.
ACCESSION   DAOQNN010000414 DAOQNN010000000
VERSION     DAOQNN010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25926_length_2843_cov_9.167862"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..2695
                     /locus_tag="PKV97_15435"
     CDS             230..2695
                     /locus_tag="PKV97_15435"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNB07326.1"
                     /translation="MFGISAAHAAQANTDADVTQPVEAADGQALEGALPRLQETVASA
                     DTILLDYQYVWDPEGNLLHARRREPDGPLPSTRRSQAYDLRNQLVTSVEWRDDGDVLA
                     ETAVWRYAYDRHQRRVLAQEGAVSQQELAGHTRPTHFAPGSHRALSSSPRAANVPARA
                     AASRPHEAATPGHHDASGQPERFGTRSLRWDALGRLIEVRAGERSIARYAYDHRGLRI
                     ERTRFDPAMVAPTTTHTVYDDARQPLAELDADGRLIRQYLWLADLPLAVLDTPAQPAT
                     ETGSARRLLEDLRRIVQSWLDPQAGLAWLHTNHLGAPELATDADGEPLWRARHAPFGA
                     ATVTTSPRRPDFTLDLRLPGQVFDAETGLHYNRRRYYEPTLGEYLTPDPLGTPDGPNP
                     YAYATFNPLRNVDPDGLVLFAFDGTGNSDDLNDPAMAGSGFSNVVYFFDAYTATKRYV
                     SGVGTVHHDVDYGDIRPEDHATGHLLWWLTPGDPVHVNDMGGNYSGPARIGRMSQYLD
                     DEAELFSDDRVMDIDIVGFSRGAAQAREFANRIVAKTVRHENQLYYRYTNRRGDSACQ
                     AVDFRFMGLFDTVLSTNFSGEAYRLGIPEVFAHVAQAVALNEHRSDSITEFAYRNPKP
                     HRMHWGGFPLESIGASSAAPGRIRIEKGFIGAHADIGGGYPDAEQGLSLVALNWMVAQ
                     ARDAGVEMESVAPVPILNVVIHDQSNVIEIGNPQHSVVPRPTGNGDLPRIDYVFPEDR
                     AVNGAVAGDRQRRMAFDNGSLTHADTLRYITWLPRDATRRGDGSTLDPRRLGDVTGTV
                     DMASYLAWLGQPENGYTTTNGGF"
BASE COUNT          478 a         1091 c          881 g          393 t
ORIGIN      
        1 cggccagcgc cgcgggcacc gggcccggat ggcgttcgag cgcgatctct tcggcctgcg
       61 tgacttccgg gccggcaacg gcatgcgtac gttgcacgag cgcacgcgca ccggcacctt
      121 ggtccgcgtc gttcatctgc gcgccgacga gcgcggcgca ccggtccgta atgcacgtgc
      181 gctgctgccg atcgcacctg gccagcccct ggccacgcgc atcgaacgct tgttcggcat
      241 ttccgcggcc cacgccgcgc aagcaaacac cgatgcggac gtcacccagc cggtggaggc
      301 agcagatggc caagccctcg aaggtgcgtt accacgcctg caggagactg tcgcgtccgc
      361 cgacaccatc ctgctcgact accagtacgt ctgggacccg gaaggcaacc tcctgcacgc
      421 gcgccggcgc gagcccgacg gcccgctgcc ctcgacgcgc cgcagccagg cctacgacct
      481 tcgcaaccag ctcgtgacca gcgtcgaatg gcgggacgac ggcgacgtgc tggccgaaac
      541 cgccgtgtgg cgttacgcct acgatcgcca ccagcgccgc gtccttgccc aggaaggcgc
      601 cgtgtcgcag caggagctcg ccggccacac ccgcccgacc cacttcgcac cgggcagcca
      661 ccgtgccctg tcctcgagcc cacgcgcagc aaacgtgccg gcgcgggccg ccgcgagccg
      721 accgcacgag gccgccacgc ccggtcacca cgacgccagc ggccagcccg aacgcttcgg
      781 cacgcgcagc ctgcgatggg acgcactcgg ccgcctgatc gaggtgcgcg caggcgagcg
      841 cagcatcgcc cgctacgcct acgaccaccg cggcctgcgc atcgagcgca cgcgcttcga
      901 tcccgcaatg gtcgcgccca ccacgaccca caccgtgtac gacgacgccc gccaacccct
      961 cgcagaactg gacgccgacg gcagattgat ccgccagtat ctgtggctcg ccgacctgcc
     1021 cctcgccgta ctcgacaccc cggcgcagcc cgccaccgag accggctccg cgcgccgcct
     1081 cctcgaagat ctgcgccgca tcgtgcagag ctggctcgac ccgcaggccg gtctcgcctg
     1141 gctgcacacc aaccacctcg gcgcccccga actggccacc gacgccgacg gcgagccgct
     1201 ctggcgcgcg cgccacgccc ccttcggcgc cgcgacggtc accacctcgc cccggcgtcc
     1261 cgacttcacc ctcgacctgc gcctgcccgg ccaggtcttc gacgccgaaa ccggcctgca
     1321 ctacaaccgc cgccgctact acgagccgac cctgggcgaa tacctcaccc ccgacccgct
     1381 cggtacgccg gacgggccga acccgtatgc gtatgcgacg ttcaatccgc tgcggaacgt
     1441 ggatccggat gggctggtgc tgttcgcgtt cgatgggacg gggaacagcg acgacctcaa
     1501 cgacccggcg atggcgggca gcggattcag caatgtggtg tatttctttg acgcttacac
     1561 tgccaccaag cgctatgtca gtggcgtcgg caccgtgcat cacgacgtcg actacggcga
     1621 catccgcccg gaagaccacg cgaccggcca tctgctctgg tggctgacac cgggcgatcc
     1681 ggtccatgtg aacgacatgg gcggcaacta ctcggggccg gcacggatcg ggcggatgag
     1741 ccaatacctg gacgacgagg ccgagctctt cagcgacgac cgggtaatgg acatcgacat
     1801 cgtcggcttc agccgcggcg cggcccaggc gcgcgagttc gccaaccgga tcgtcgcgaa
     1861 gacggtccgc catgagaacc agctctatta ccgctacacg aaccgccgcg gagactctgc
     1921 ctgtcaggcg gtcgatttcc gcttcatggg gctcttcgac accgtgctat cgaccaattt
     1981 cagcggcgaa gcgtatcgcc tcgggatccc ggaggtcttc gcccatgtcg cgcaggcggt
     2041 cgcgctcaac gagcaccgct cggactcgat cacggagttc gcctatcgca acccgaagcc
     2101 gcaccgcatg cactggggcg gctttccgct cgagtcgatc ggcgccagca gcgctgcgcc
     2161 cggtcgcatc cgcatcgaga agggtttcat cggcgcccat gcggacatcg gcgggggcta
     2221 tcccgacgcc gagcaaggcc tctcgctggt tgcactcaac tggatggtgg cgcaagcacg
     2281 cgacgccggg gtagaaatgg agtcggtggc ccccgttccc atccttaatg tggtgatcca
     2341 cgaccagagc aatgtgatcg agatcggcaa cccgcaacac agcgtcgttc cgcgcccgac
     2401 cgggaacggc gatctgcccc ggatcgatta cgtcttcccc gaggaccgcg cggtcaacgg
     2461 cgcggtggcc ggagatcgcc agcgccgcat ggcgttcgac aacggcagcc tcacccacgc
     2521 cgacaccctg cgctacatca cctggctccc gcgcgacgcc acaaggcgcg gcgacggctc
     2581 cacgctcgac ccgcgccgtc tcggcgacgt caccggcacg gtggatatgg cgagctacct
     2641 cgcctggctc gggcagcccg agaacggcta cacgacgaca aatggaggat tctgatgcga
     2701 cgacgcttcc tgatccggct cgcggtggcg accctcggca ccgcccttat gacggcctgt
     2761 cgcgggaccc tgccgacagc cccgcccgcc ccgctcaccg aatcccaggc gcgctacctg
     2821 gagagccggc agcgcatgct gca
//
LOCUS       DAOQNN010000415         2827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_26176_length_2827_cov_12.498196, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000415 DAOQNN010000000
VERSION     DAOQNN010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26176_length_2827_cov_12.498196"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="PKV97_15440"
     CDS             <1..178
                     /locus_tag="PKV97_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB07327.1"
                     /translation="LAADAPPISAAKRLDQLEIELIERVMREVGGNLSAAARTLGVSR
                     NRLYRKLGRAGGVS"
     gene            309..788
                     /locus_tag="PKV97_15445"
     CDS             309..788
                     /locus_tag="PKV97_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB07328.1"
                     /translation="MWKALHIDPAKCTGCLQCEMACSYEHTGVINPSKSRIKVFNFEH
                     EGRKVPYTCTQCTEAWCLNSCPVDAIRIDLSTGAKMVFEDTCVGCKVCTIACPFGTIN
                     YNQDSGKVQKCDLCEGNPACASACPTGAITYVDADWTGIDKMRAWAAKANTPASAAA"
     gene            801..2648
                     /locus_tag="PKV97_15450"
     CDS             801..2648
                     /locus_tag="PKV97_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNB07329.1"
                     /translation="MGWNRKVLRVNLTAGTCTEEALNMQWADEYLGSRGLATKYLVSE
                     IDPRVDPLSPDNKMIMATGPLTGTMASTGGRYTVVTKGPLTGAIACSNSGGFFGAEMK
                     FAGWDMVIFEGKSAKPVWLYIENDKAELRDASGLWGRSCWETEEKIKEQLQDPLVRIS
                     SIGVSGENQVLYSCIVNDLHRAAGRSGVGAVMGSKNLKAVAIRGTKGVSGIKDFGAFL
                     KATNDAKKVLADNAVTGQGLPKYGTQVLMNVINEIGALPTRNHRDVQFEDASKISAEA
                     MHEKRASDGKTHLVTNAACFGCTIACGRISEIDKTHFTVKNSPKYWGASGGLEYEAAW
                     ALGAANGVGDLEALQYANLICNEQGMDPISFGATVGAAMELYELGILTDEKIGMAAPF
                     GSAEALAKMVEMTALGEGFGKELGLGSKRLCEKYGRPELSMSVKGQEFPAYDSRGIQG
                     MGLAYATSNRGACHLRGYTVASEVLGIPVKTDPLTTDGKPELVKAFQDATAVFDSAGI
                     CVFTSFAWTLADVQPQVAAACEGDWSMEKLNEVGERIWNMERMFNNAAGFTAKDDNLP
                     PRLTQEPAKTGPAKGLVNGLAKMLPEYYQVRGWTPGGEPTAETRARLGL"
BASE COUNT          521 a          937 c          959 g          410 t
ORIGIN      
        1 gctggcggcc gacgcgccgc cgatttccgc cgcgaaacgc ctggaccagc tcgagatcga
       61 gttgatcgag cgcgtcatgc gcgaggtcgg cggcaacctg tcggcggcgg cgcgcacgct
      121 cggggtcagc cgcaaccgcc tctatcgcaa gctcggccgc gccggcgggg tgtcctgagt
      181 ggggtaatcc cgccttgccc gcgggcaggt atgacttatg cttgtacttg cataggtgag
      241 cagtggggcg tactgcccgc agcactgtga ccgagcgcag gaatcgataa acgacaagga
      301 gacgatcgat gtggaaagca ctgcacattg atcccgcgaa gtgcaccggc tgtctgcaat
      361 gcgagatggc ctgttcctac gagcacaccg gggtcatcaa tccgagcaag tcgcgcatca
      421 aggtgttcaa cttcgagcac gagggccgca aggtgcccta cacctgcacg cagtgcaccg
      481 aggcctggtg cctgaactcc tgtccggtgg atgcgatccg catcgacctg tcgaccggcg
      541 ccaagatggt gttcgaggac acctgcgtcg gctgcaaggt gtgcacgatc gcctgtccgt
      601 tcggcaccat caactacaac caggacagcg gcaaggtgca gaagtgcgac ctgtgcgagg
      661 gcaaccccgc ctgcgccagc gcctgtccga ccggggcgat cacctatgtg gacgccgact
      721 ggaccggcat cgacaagatg cgcgcctggg ccgcgaaggc caacaccccg gcttcggccg
      781 ctgcctgagg aggatgcgtc atgggatgga atcgcaaagt cctgcgcgtg aacctcaccg
      841 cagggacctg taccgaagaa gcgctgaaca tgcagtgggc cgacgaatac ctcggctcgc
      901 gcgggctggc caccaagtac ctggtgtccg agatcgaccc cagggtcgac ccgctgtcgc
      961 ccgacaacaa gatgatcatg gccaccgggc cgctcaccgg caccatggcc tcgaccggtg
     1021 gccgctacac cgtggtcacc aaggggccgc tgaccggcgc catcgcgtgc tcgaactcgg
     1081 gcggcttctt cggcgccgag atgaagttcg ccggctggga catggtgatc ttcgagggca
     1141 agtcggccaa gccggtatgg ctctacatcg agaacgacaa ggccgagctg cgtgacgctt
     1201 ccggcctgtg gggcaggagc tgctgggaaa ccgaggagaa gatcaaggag caactccagg
     1261 acccgctggt acgcatctcc tcgatcggcg tctcgggcga gaaccaggtg ctctattcct
     1321 gcatcgtcaa tgacctgcac cgtgccgccg ggcgctccgg cgtgggtgcg gtgatgggct
     1381 cgaagaacct caaggcggtg gcgatccgcg gcaccaaggg cgtctccggc atcaaggact
     1441 tcggcgcctt cctcaaggcc acgaacgacg ccaagaaagt gctcgccgac aacgcggtca
     1501 ccggccaggg cctgcccaag tacggcaccc aggtgctgat gaacgtgatc aacgagatcg
     1561 gcgcgctgcc cacgcgcaac caccgcgacg tgcagttcga ggacgcctcg aagatctccg
     1621 ccgaggccat gcacgagaag cgcgcgtccg acggcaagac ccacctcgtc accaatgccg
     1681 cctgcttcgg gtgcacgatc gcctgcgggc gcatctccga gatcgacaag acccacttca
     1741 cggtcaagaa cagcccgaag tactggggcg cctccggcgg cctggagtac gaagcggcgt
     1801 gggcgctcgg cgcggccaac ggcgtgggcg acctcgaggc gctgcagtac gccaacctga
     1861 tctgcaacga gcagggcatg gacccgatct ccttcggcgc caccgtcggt gcggcgatgg
     1921 agctctacga gctggggatc cttaccgacg agaagatcgg catggcggcg cccttcggct
     1981 cggccgaggc gctggcgaag atggtcgaga tgaccgcgct cggcgagggt ttcggcaagg
     2041 agctcggcct gggcagcaag cgcctgtgcg agaagtacgg ccgccccgag ctgtcgatga
     2101 gcgtgaaggg gcaggaattc cccgcctacg actcgcgcgg catccagggc atgggcctgg
     2161 cctacgccac ctccaaccgc ggcgcctgcc acctgcgcgg ctataccgtg gcgtcggagg
     2221 tgctcggcat cccggtcaag accgacccgc tcaccaccga tggcaagccc gagctggtga
     2281 aggccttcca ggacgccacc gcggtgttcg actccgccgg gatctgcgtg ttcaccagct
     2341 tcgcgtggac cctggccgac gtgcagccgc aggtcgccgc ggcgtgcgag ggcgactgga
     2401 gcatggagaa gctcaacgag gtgggcgagc gcatctggaa catggagcgc atgttcaaca
     2461 acgcggccgg cttcaccgcc aaggatgaca acctgccgcc gcgcctcacc caggaaccgg
     2521 ccaagaccgg cccggccaag ggcctggtca acggcctggc gaagatgctg cccgagtact
     2581 atcaggtgcg cggatggacg ccgggtggcg agccgaccgc ggagacacgg gcacgcctgg
     2641 gcctgtgatt cagcaaacgc ccatggacgc ccccgcgggc gcccatggcg ctggattgcc
     2701 ctgcaggagc ggcgtcagcc gcgagggtct ggacgggcgc ggttggcgcc gcgcacgctc
     2761 ggcccgatcc gaagggcgcg cggcttgcgc cgctcctacg gggcccggat cacccggatt
     2821 acctgcg
//
LOCUS       DAOQNN010000416         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_26210_length_2825_cov_11.307581, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000416 DAOQNN010000000
VERSION     DAOQNN010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26210_length_2825_cov_11.307581"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="PKV97_15455"
     CDS             complement(<1..132)
                     /locus_tag="PKV97_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNB07330.1"
                     /translation="MSNFVTPEQFTAANKANIETLLTLANTAFASAERLAALNLNTAR
                     "
     gene            complement(461..1237)
                     /locus_tag="PKV97_15460"
     CDS             complement(461..1237)
                     /locus_tag="PKV97_15460"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNB07331.1"
                     /translation="MSFEMILAEKRGRVGLITLNRPKALNALNDQVVNEITAALNDFE
                     SDDGIGAIVITGSEKAFAAGADIGAMANFSYMDAYKSEYITRNWERVKTCRKPVIAAV
                     AGFALGGGSELAMMCDIIIAADTAKFGQPEVKLGTLPGAGGTQRLPRAVGKAKAMELC
                     LTGRMMDAVEAERAGLVARVVPADKLLEDALATAETIAGYSLPVVMMIKESINRAFES
                     SLNEGLLFERRVFHSAFALNDQKEGMAAFVEKRKANFRHD"
     gene            complement(1275..1817)
                     /gene="lptA"
                     /locus_tag="PKV97_15465"
     CDS             complement(1275..1817)
                     /gene="lptA"
                     /locus_tag="PKV97_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HNB07332.1"
                     /translation="MKRILLATVAALLAAVTTPALAEKADRDQPVNIEADRLTVDDRN
                     KVHIFEGSVVLTQGTLTIRGDKLVVTQDAAGFQNGVATAAGKGLATFRQRRDGSAEYV
                     EGEAERIEYDSRTEKARLFNRAKVSSGGDQVSGHYIEYDAITENYLATNAPGSRQGAP
                     AGRVRAVIQPKSDGQPSGGQ"
     gene            complement(1814..2395)
                     /gene="lptC"
                     /locus_tag="PKV97_15470"
     CDS             complement(1814..2395)
                     /gene="lptC"
                     /locus_tag="PKV97_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNB07333.1"
                     /translation="MRVDTWRLYPVFALALLAGGSVWLERITRIDDPAASIEQTGPDF
                     IAHDTRVVGFGADGAQRYELVAERLEHFPVGDITRLHTPRLQLHGAEGTTTITARAAD
                     VSPGGEQVDLTGEVRVRRPGQAGALPLALDSETLAVWPDAQRARTDSPVLMTRGAGRA
                     TAKGMRADNLFGTLELIGEVSTTMPPRRQGSPS"
     gene            complement(2396..>2825)
                     /locus_tag="PKV97_15475"
     CDS             complement(2396..>2825)
                     /locus_tag="PKV97_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB07334.1"
                     /translation="DFARSHPGGALGRRLLTHVGDVMRPAPAVPRVGSDAPLTQALLA
                     MTAGGMGMTAVVDADEVPVGIFTDGDLRRALEKGCDVRSARVSEVMTRSPRSIAPGAL
                     AAEAAATMENMRISQLLVLDDAGRLAGALTTHDLMLAKVI"
BASE COUNT          376 a          911 c         1043 g          495 t
ORIGIN      
        1 acgggcggtg ttcaggttca gggcggcgag gcgctcggcg ctggcgaagg cggtgttggc
       61 cagggtcagc agggtctcga tgttggcctt gttggcggcg gtgaactgct cgggggtgac
      121 gaagttgctc atgtggatct ccttggaaag tgtgggtgca tcaacatcgc tcgggtagct
      181 gcgatgttgc gttgcagcat ggttcggatt atacggaccc gatcgaggct gtcaagcata
      241 ttttggtgca tcgcaacatc ccggattgat tgaaggaatc aatcacttgc gccaggacgc
      301 catttcgtcc ggtgaagggt tcggggtgtt gcattgcacg ggcggcgagt acgccgaagc
      361 agtgtgccgg gcacgggttg caggcgcatg acgtcttccc gcgggtaaac gcaagcggcc
      421 ggctggacgc cggccgcggt gcggatcgag cgcgggacga tcagtcgtgg cggaagttcg
      481 ccttgcgctt ctcgacgaag gccgccatgc cttccttctg gtcgttgagc gcgaacgcgg
      541 agtggaagac gcggcgctcg aacagcaggc cctcgttgag gctgctctcg aaggcgcggt
      601 tgatcgattc cttgatcatc atgaccaccg gcagcgagta gccggcgatg gtttccgcgg
      661 tggcgagcgc gtcctcgagc agcttgtcgg ccggcaccac gcgcgcgacc aggccggcgc
      721 gctcggcttc gaccgcgtcc atcatgcgcc cggtcaggca cagctccatc gccttcgcct
      781 tgcccaccgc gcgcggcagg cgctgcgtgc cgcccgcacc gggcagggtg ccgagcttga
      841 cttccggctg gccgaacttc gcggtgtccg cggcgatgat gatgtcgcac atcatcgcca
      901 gctcgctgcc gccgccgagc gcaaagcccg ccaccgcggc gatcaccggc ttgcggcagg
      961 tcttcacgcg ctcccagttg cgcgtgatgt actcgctctt gtaggcatcc atgtaggaga
     1021 agttcgccat cgcgccgatg tcggcgcctg cggcgaaggc cttctccgag ccggtgatga
     1081 cgatggcacc gatgccatcg tcggactcga agtcgttgag ggcggcggtg atctcgttca
     1141 ccacctgatc gttgagcgcg ttgagcgcct tggggcggtt gagggtgatg aggccgacac
     1201 ggccgcgttt ctcggcgagg atcatctcga agctcatgct gtctccttcc tggttatccg
     1261 atcgtggggg ggaatcattg tccgccggag ggctgtccgt cggacttggg ctggatcacc
     1321 gcgcgcaccc ggcccgcggg cgcaccctgg cggctgcccg gggcgttggt ggcgagatag
     1381 ttctcggtga tcgcgtcgta ctcgatgtag tggccgctga cctggtcgcc gccgctgctc
     1441 accttggcgc ggttgaagag cctggccttc tcggtccggc tgtcgtactc gatgcgttcg
     1501 gcctcgcctt cgacgtattc ggcgctgccg tcgcgacgct ggcggaaggt ggccaggccc
     1561 ttgcccgccg cggtggcgac gccgttctgg aagccggcgg cgtcctgcgt cacgaccagc
     1621 ttgtcgccgc ggatggtgag cgtgccctgg gtcaggacca cgctgccctc gaagatgtgc
     1681 accttgttgc ggtcgtccac cgtgaggcgg tcggcctcga tgttgaccgg ctggtcgcgg
     1741 tcggccttct cggcgagcgc cggggtggtg acggcggcga gcagcgcggc cacggtagcg
     1801 aggaggattc gcttcatgag ggggatcctt ggcggcgggg tggcatcgtc gtgctgacct
     1861 cgccgatgag ttcgagcgtg ccgaacaggt tgtccgcacg catgcccttg gccgtcgcgc
     1921 gtccggcgcc gcgggtcatc aggaccggcg agtcggtgcg cgcgcgctgg gcgtccggcc
     1981 acaccgcgag cgtctccgag tcgagcgcga gcggcagcgc cccggcctgg cccggacgcc
     2041 gcacgcgcac ttcgccggtg aggtccacct gctcgccacc gggcgagacg tcggccgcgc
     2101 gcgcggtgat cgtggtcgtg ccttccgcgc cgtgcagctg caggcggggc gtgtgcagcc
     2161 gggtgatgtc gccgacgggg aaatgctcca ggcgttcggc cacgagctcg tagcgctgcg
     2221 caccgtcggc gccgaagccg accacgcggg tgtcgtgggc gatgaagtcc ggaccggtct
     2281 gctcgatgct cgccgccgga tcgtcgatcc gggtgatgcg ctcgagccac accgatccgc
     2341 ccgccagcag cgcgagggcg aagaccggat acaggcgcca ggtgtcgacc cgcattcaga
     2401 tgaccttcgc gagcatcagg tcgtgggtgg tgagcgcacc ggcgagccgg ccggcgtcgt
     2461 cgagcaccag gagctggctg atgcgcatgt tctccatcgt cgcggcggcc tcggccgcga
     2521 gcgcgccggg cgcgatgctg cgcggactgc gcgtcatgac ctcgctgacg cgcgcgctgc
     2581 gcacgtcgca gcccttctcc agcgcgcgcc gcaggtcgcc gtcggtgaag atgcccacgg
     2641 gcacctcgtc ggcgtcgacc accgcggtca tgcccatgcc gccggcggtc atcgccagca
     2701 aggcctgggt gaggggggcg tcgctgccca cgcgcgggac cgccggcgcg ggacgcatga
     2761 cgtcgccgac gtgggtcagc agccggcgac cgagcgcgcc gccggggtgg gagcgggcga
     2821 aatcc
//
LOCUS       DAOQNN010000417         2809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_26468_length_2809_cov_11.878359, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000417 DAOQNN010000000
VERSION     DAOQNN010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26468_length_2809_cov_11.878359"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="PKV97_15480"
     CDS             <1..1175
                     /locus_tag="PKV97_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNB07335.1"
                     /translation="ALARTVTQQAAGKDRTLSMLLGRIAVHRLWGWPMLLGVLYLVYL
                     FVGDFGASTLVGLLEEDFFGEVLNPAVTEFVTSHISTPWLADLIVGEYGLWTMGMTYA
                     LALILPIVTTFFLAFGVLEDSGYFSRLSVIANRMFAAIGLNGRAVLPMVLGLGCVTMA
                     TLTTRILHTPRERLITIYLMALAIPCSAQLGVVLGLLGGISFGATLIWVLAIVGVLLF
                     TGWLASKVVPGRRVPLVTELPPMRMPVLGNVLKKTGGRLKWYLIEVIPLFLIGTLIMF
                     GLDRSGALPWIIEAGEPLVVGWLGLPPEASAAFVMGFLRRDFGASGLFALQSELSMIQ
                     AVVGMVTITLFVPCIASLMMIIKEQGMKVALLMLAMIIPTAFLVGGLLNHGLHLIF"
     gene            1188..1448
                     /locus_tag="PKV97_15485"
     CDS             1188..1448
                     /locus_tag="PKV97_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HNB07336.1"
                     /translation="MNAAPSKPLPHERIRLIGTPVGRMVRIDDFDDEIDPLQREQLHA
                     YGLSAAHAVKVLQQQPMTVLLCEHVELAIEHAVSRHIWVQPA"
     gene            1596..>2809
                     /locus_tag="PKV97_15490"
     CDS             1596..>2809
                     /locus_tag="PKV97_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB07337.1"
                     /translation="MSQNAYQQGLDKNAANYVPLSPLSFIERTAMVYPTRTSVVHGTL
                     IYTWSQTYERCRRLASALVAHGVKKGDTVACMLPNVPSMFEAHFGVPMTGAVLNTLNT
                     RLDADAIAFMLQHGEAKVLITDPEFAGVVRAALELLPGDKPLVVDALDPEYPGTERIG
                     TVLYEDFLAGGNPDFQWKLPEDEWDAIALNYTSGTTGNPKGVVYHHRGAYLNAASNII
                     SWGMPQHSSYLWTLPMFHCNGWCFPWTMAANAGVNVCLRKVDPALIYELIRRHKVTHM
                     CGAPIVYGMLINAPDGLRAGIEHSVAGLIAGAAPPAAIIEGAEKIGFDITHVYGLTET
                     YGPASVCAKHPEWDALPIDRRAERNGRQGVRYHMQEAITVLDPVTMEPVPADGETMGE
                     IMFRGNLVMKGYL"
BASE COUNT          455 a         1015 c          874 g          465 t
ORIGIN      
        1 aggcgctcgc gcgcaccgtc acccagcagg ctgccggcaa ggaccgcacg ctgtcgatgc
       61 tgctcggccg catcgcggtg caccggctgt ggggctggcc gatgctgctc ggcgtgctgt
      121 atctggtgta tctgttcgtc ggcgacttcg gcgccagcac cctggtcgga ctgctggaag
      181 aagacttctt cggcgaggtc ctcaaccccg cggtcaccga gttcgtcacg agccacatct
      241 ccacgccctg gctggccgac ctgatcgtgg gcgagtacgg cctgtggacc atgggcatga
      301 cctacgcgct cgcgctgatc cttcccatcg tcaccacctt cttcctcgcc ttcggcgtgc
      361 tcgaggattc gggctacttc tcgcgcctgt cggtgatcgc caaccgcatg ttcgccgcca
      421 tcggcctcaa cggccgcgcg gtgctgccga tggtgctcgg cctgggctgc gtgaccatgg
      481 ccacgctcac cacccgcatc ctgcacaccc cgcgcgagcg cctgatcacc atctacctga
      541 tggcgctggc gatcccgtgc tctgcgcaac tcggcgtggt gctcggcctg ctcggcggca
      601 tctccttcgg cgccacgctg atctgggtgc tggcgatcgt cggcgtgctg ctgttcaccg
      661 gctggctggc gagcaaggtc gtgcccggcc gccgcgtgcc gctcgtcacc gagctaccgc
      721 cgatgcgcat gccggtgctc ggcaacgtgc tcaagaagac cggcggaagg ctgaagtggt
      781 atctgatcga ggtgatcccg ctgttcctga tcggcaccct gatcatgttc gggctcgacc
      841 gcagcggcgc gctgccgtgg atcatcgagg ccggcgagcc gctggtggtg ggctggctcg
      901 gcctgccgcc ggaggcgagc gccgccttcg tgatgggctt cctgcgccgc gacttcggcg
      961 ccagcggcct gttcgcgctg cagagcgagc tgtcgatgat ccaggccgtg gtgggcatgg
     1021 tcacgatcac gctcttcgtg ccctgcatcg ccagcctgat gatgatcatc aaggagcaag
     1081 gcatgaaggt cgccctgctg atgctcgcaa tgatcatccc caccgccttc ctggtcggcg
     1141 gcctgctcaa ccacggcctg cacctgatct tctgaggaat ccgcgccatg aacgccgccc
     1201 cctccaaacc cctgccccac gagcgcatcc gcctgatcgg caccccggtc gggcgcatgg
     1261 tgcgcatcga cgacttcgac gacgagatcg acccactgca gcgcgagcag ctccacgcct
     1321 acgggctctc cgccgcgcac gcggtgaagg tgctgcaaca gcaaccgatg acggtgctat
     1381 tgtgcgagca cgtcgagctc gcgatcgagc acgcggtgtc gcgccatatc tgggtgcagc
     1441 ccgcctgagc gctccaacct gccttcgcaa acgttgcgcg cgggagaact gcagacaaga
     1501 ccgacgccat cctccatact atacggtatg gaccgaaccc cgcgtgggac gccggcccga
     1561 ccagtttgtt tgacaatgaa cgggagagga aaaggatgtc gcaaaacgca tatcagcaag
     1621 gcctcgacaa gaacgcggcc aactacgtgc cgctgtcccc gctgagcttc atcgagcgca
     1681 ccgccatggt ctatccgacg cgcaccagcg tcgtccatgg cacgctcatc tacacctgga
     1741 gccagaccta cgagcgctgc cgccgcctcg cgagcgcctt ggtcgcgcac ggcgtgaaga
     1801 agggcgacac ggtcgcgtgc atgctgccca acgtgccgtc gatgttcgaa gcgcatttcg
     1861 gcgtgccgat gaccggcgcg gtcctcaaca cgctcaacac gcgcctcgac gccgatgcca
     1921 tcgccttcat gctgcagcac ggcgaagcca aggtgctgat caccgacccc gagttcgccg
     1981 gcgtggtccg cgccgcgctc gagctgctgc cgggagacaa gcccctggtg gtcgacgccc
     2041 tcgaccccga gtatccgggc accgagcgca tcggcaccgt gctctacgag gacttcctcg
     2101 ccggcggcaa ccccgacttc cagtggaagc tgcccgagga cgagtgggac gcgatcgcgc
     2161 tcaactacac ctccggcacc accggcaacc cgaagggcgt ggtctaccac caccgcggcg
     2221 cgtatctcaa cgccgcctcc aacatcatca gctggggcat gccgcagcac tcgtcctacc
     2281 tgtggacgct gccgatgttc cactgcaacg gctggtgctt cccgtggacc atggcggcca
     2341 acgccggcgt caacgtctgc ctgcgcaagg tcgacccggc gctgatctac gagctgatcc
     2401 gccgccacaa ggtcacccac atgtgcggcg cgccgatcgt ctacggcatg ctgatcaacg
     2461 cccccgacgg cctgcgcgcc ggcatcgagc acagcgtcgc cggcctcatc gccggcgccg
     2521 cgcccccggc cgcgatcatc gagggcgccg agaagatcgg cttcgacatc acccacgtct
     2581 atggcctgac cgaaacctac ggcccggcct cggtgtgcgc caagcacccc gagtgggatg
     2641 cgctgccgat cgaccgccgc gccgagcgca acggccgcca gggcgtgcgc taccacatgc
     2701 aggaagcgat caccgtgctc gacccggtga ccatggagcc ggtgcccgcc gacggcgaga
     2761 ccatgggcga gatcatgttc cgcggcaacc tggtgatgaa gggctacct
//
LOCUS       DAOQNN010000418         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_26557_length_2803_cov_8.921397, whole genome shotgun sequence.
ACCESSION   DAOQNN010000418 DAOQNN010000000
VERSION     DAOQNN010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26557_length_2803_cov_8.921397"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKV97_15495"
     CDS             <1..262
                     /locus_tag="PKV97_15495"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNB07338.1"
                     /translation="YGVRKINIDTDIRLAMTGAIRKFMFENPSKFDPREYNKPAREAA
                     KLVCKARYEAFGCAGMASKIKPVALDKIAARYKAGELSQIVR"
     gene            396..1697
                     /locus_tag="PKV97_15500"
     CDS             396..1697
                     /locus_tag="PKV97_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HNB07339.1"
                     /translation="MSSLFEQHPLRQTLSDEVHARPPVPLDTPEFVTYLAFLHHEGSA
                     GREADHLAALAGQLGQPAPDIDSGHVLLEAEGFRLKWERHNEFSSYCFFRRIEPDDSP
                     DDYALLHVPAAWRKAIPGLLIAATHIELRDVGEVPLEAVLHQQSRHGQALVASGVSDG
                     AGWVLTDFHLHDGFSHFLLLDKGFTPRQAGRIAQRLVEIETYRVMALLAFPVAKDTGR
                     LVSRAEDELADLMDGMGRSRSAEDDRAVLARLSRLAAEVERSVARTTFRFGAAAAYYR
                     LVRQRIDDLREQRLPGFSPIGEFMDRRLVPAIDTCTSIARRQDDLSGRIARNSQLLRT
                     RVDIEIERQNQELLAQMNRRAKIQLHLQETVEGLSVVAITYYASQLVNYVAKGAKHLI
                     EPLTPEVLTAVSIPVIAGIVFMGLRRMRKLLGTEEDGQAHP"
     gene            complement(1723..>2803)
                     /locus_tag="PKV97_15505"
     CDS             complement(1723..>2803)
                     /locus_tag="PKV97_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HNB07340.1"
                     /translation="SALAGLGVAGGAGVLSHRQSVAVYEAAAGTTWRHGEPAQRDTRA
                     ALRELVRYATLAPSSHNTQCWKFALAERSVSILPDYSRRCPVVDPDDHHLFVSLGCAA
                     ENLVQAAPAMGFHCEAAFDGEAAEVLNCALAPAPSRRTALFEAIPQRQSTRGEFDSRP
                     LSNEELRRLEAAGSGRGVSVRLLTGAQALERVLEFVVAGNTAQMRDPAFVRELKAWIR
                     FNGSDAARTGDGLFAGASGNPAAPAWLGGLLFDAFFTEAAENDKYARQIRSAAGIAVF
                     VSERDDRAHWIEAGRCFQRFALQATAIGVRTAHLNQPVELATLRPAFAADLGITNGRP
                     DLVIRFGRGPAMPRSLRRPLDAVLL"
BASE COUNT          432 a         1069 c          924 g          378 t
ORIGIN      
        1 gtacggcgtg cgcaagatca acatcgacac cgacatccgc ctcgccatga ccggggcgat
       61 ccgcaagttc atgttcgaga acccgtcgaa gttcgacccg cgcgagtaca acaagcccgc
      121 gcgcgaggcc gccaagctgg tctgcaaggc gcgctacgaa gccttcggct gcgccggcat
      181 ggcctcgaag atcaagcccg tcgcgctcga caagatcgcg gcgcgttaca aggccggcga
      241 gctgagccag atcgtgcgct gaaccagcgc ggagcacatg cttgaagcga cgaccgcgcc
      301 agctggcgcg gtcgtcgctt caagcggccg tggatccatt gtcttcgatc gcgtcgccct
      361 catccaaccc gccgccgact gctctgcgag ccaccatgtc ctccctcttc gaacagcacc
      421 ccctgcgcca gacgctcagc gacgaggtgc acgcgcgccc gccggtgccg ctggacaccc
      481 cggaattcgt cacctacctc gccttcctgc accacgaagg cagcgccggg cgcgaggcgg
      541 accacctcgc ggcgctggcc gggcagctcg gccagcccgc gccggacatc gactcgggcc
      601 acgtgctgct cgaggccgag ggtttccggc tgaagtggga gcgccacaac gagttctcca
      661 gctactgctt cttccgccgc atcgagcccg acgacagccc cgacgactac gccctgctgc
      721 acgtgcccgc ggcctggcgc aaggccatcc ccggcctgct gatcgccgcc acccacatcg
      781 agctgcgcga cgtcggcgag gtgccgctgg aggcggtgct gcaccagcag tcgcgccacg
      841 gccaggcgct ggtcgcctcc ggcgtctcgg acggcgccgg ctgggtgctc accgacttcc
      901 acctgcacga cggcttctcg cacttcctgc tgctcgacaa gggcttcacc ccgcgccagg
      961 ccggccgcat cgcccagcgc ctggtcgaga tcgagaccta ccgcgtcatg gcactgctcg
     1021 ccttcccggt ggccaaggac accggccgcc tggtctcgcg cgccgaggac gagctcgccg
     1081 acctcatgga cggcatgggc cggtcgcgca gcgccgagga cgaccgcgcg gtgctcgccc
     1141 gcctgtcgcg cctggccgcc gaggtcgagc gctcggtggc gcgcaccacc ttccgcttcg
     1201 gcgccgccgc ggcctactac cgcctggtgc gccagcgcat cgacgacctg cgcgaacagc
     1261 gcctgcccgg cttctccccg atcggcgagt tcatggaccg ccgcctcgtc cccgcgatcg
     1321 acacctgtac ctcgatcgcg cgccgccagg acgacctctc cggccgcatc gcacgcaact
     1381 cgcagctgct gcgcacccgc gtcgacatcg agatcgagcg ccagaaccag gagctgctcg
     1441 cgcagatgaa ccgccgcgcc aagatccagc tccaccttca ggagaccgtt gaagggctgt
     1501 cggtggtggc gatcacctac tacgcctcgc agctggtgaa ctacgtcgcc aagggcgcaa
     1561 agcacctgat cgagccgctg acgccggagg tgctcaccgc ggtgtcgatc ccggtcatcg
     1621 ccggcatcgt cttcatgggg ctgcggcgga tgcgcaagct gctgggcacg gaagaggacg
     1681 gccaggccca cccttgaggt ggccggcggc gggcgccgcc cctcacagca gcacggcgtc
     1741 gagcggacgg cgcagggatc ggggcatcgc cggccccctg ccgaaacgga tcaccagatc
     1801 cggccggccg ttcgtgatgc ccaggtcggc ggcaaaggcg gggcgcagcg tggcgagctc
     1861 gaccggctgg ttgagatgcg ccgtgcggac gccgatcgcg gtcgcctgca gcgcaaagcg
     1921 ctggaagcaa cgccccgcct cgatccagtg cgcgcgatcg tcgcgctccg agacgaacac
     1981 cgcgatcccc gcagcactgc ggatctgccg cgcgtacttg tcgttctcgg ccgcctcggt
     2041 gaagaaggca tcgaacagca ggccgcccag ccaggcgggg gccgcgggat ttccggacgc
     2101 ccctgcgaac aggccgtcgc cggtgcgcgc cgcatcgctg ccgttgaagc ggatccacgc
     2161 cttcagctcg cgcacgaacg ccgggtcccg catctgcgcc gtgttcccgg cgacgacgaa
     2221 ctccaggacg cgttccaacg cctgcgcccc ggtgagcagg cgcacgctca cgcccctgcc
     2281 cgagccggcc gcctcgagcc gccgcagctc ctcgttcgaa agcggccgcg agtcgaactc
     2341 gccgcgggtc gattgacgct gcggaatggc ctcgaacagg gcggttcgtc ggctcggcgc
     2401 gggcgccagc gcgcagttca ggacctcggc ggcctccccg tcgaaggcgg cctcgcagtg
     2461 gaagcccatc gccggcgccg cctgcacgag gttctcggca gcgcagccca ggctgacgaa
     2521 gaggtggtga tcgtcgggat ccaccaccgg gcagcgccgc gagtagtcgg ggagaatgga
     2581 aaccgaccgc tccgccagcg cgaacttcca gcactgcgtg ttgtggctgg acggcgcaag
     2641 ggtggcgtag cgcacgagct cgcgcagtgc cgcacgcgtg tcccgctgcg ccggctcgcc
     2701 atggcgccac gtcgtacccg ctgctgcctc gtagaccgcc acgctctgcc gatgggaaag
     2761 gacgccggcc ccgcccgcca cccccagccc ggccagcgcc gac
//
LOCUS       DAOQNN010000419         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_26638_length_2798_cov_10.705797, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000419 DAOQNN010000000
VERSION     DAOQNN010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26638_length_2798_cov_10.705797"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1281
                     /locus_tag="PKV97_15510"
     CDS             82..1281
                     /locus_tag="PKV97_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB07341.1"
                     /translation="MRWRDALHLALRAITAHRLRSLLTLLGIGVGIAAVILLTSIGEG
                     LHRFVLAEFGQFGTNVINLHPGRQGARGGPPGLPSTARDLTLDDADALARLPHVRHVT
                     GSVSGNAEVRAQGRVRRSTVLGVGPQMQEVYSMRVRVGQFLPPDEAASARAFVVLGPK
                     LARELFGSTIALGEHVEIGAERFRVVGVMEEKGQFLGIDLDDAAYIPVVRGMALYQRD
                     GLMEIALTYDPEAPAARVAEAVKKRMIARHGREDFTVLTQEDMLATLSNILDLLTAAV
                     GALGAISLLVGGVGIVTIMSIAVTERTGEIGLLVALGARRRTILALFLGEAVVLAGIG
                     GLLGLLVGAGLAQLVGLLVPAMPVATPWRYALAAEGVAIVVGLAAGVLPARRAARLDA
                     VEALRAE"
     gene            complement(1414..2733)
                     /locus_tag="PKV97_15515"
     CDS             complement(1414..2733)
                     /locus_tag="PKV97_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNB07342.1"
                     /translation="MPFKVFTARDLDRIPQLARLSPEQRFEMKVVAAVLPFRVNQYVI
                     DELIDWADVPRDPIFQLTFPQRGMLASEHYERIARLIEGDADKDALEAAIAEVRHALN
                     PHPADQMQMNMPLDEHGKRIDGLQHKYRETVLFFPSQGQTCHAYCSFCFRWAQFVGDK
                     ELRIASSEARVLHDYLRTHTEVTDLLVTGGDPMVMKTRHLREYLEPLLRPEFDHIQTI
                     RIGSKALTFWPHRFLGAEDADDLMRLLRQLVEAGKHVALMAHYNHWKELETDAAHAAI
                     RRIRATGAVIRAQGPLIAHINDDPAAWARLWKTEVRLGLVPYYMFVERDTGARHYFEV
                     PLARAWEIYQQAIQQVSGLARTARGPSMSASPGKVEIQGVTEIAGEKVFVLRFIQGRN
                     PDWVQRPFFARFDEKATWLDQLAPAFGEAKWFWQDEYEAIRAERLVG"
BASE COUNT          375 a          905 c         1078 g          440 t
ORIGIN      
        1 cggggtgctg ttcggcgtgc tgcccgcgcg ccgcgccgcc cggctcgatc cggtgcaggc
       61 cttgtcgaag cggtagcggc gatgcgctgg cgcgacgccc tccacctcgc cctgcgtgcg
      121 atcaccgcgc atcggctgcg cagcctcctg accctgctcg gcatcggcgt gggcatcgcc
      181 gcggtgatcc tgctcacctc gatcggcgag ggtctgcacc gcttcgtgct cgccgagttc
      241 ggccagttcg gcaccaacgt gatcaacctc cacccgggcc gccagggcgc acgcggcggc
      301 ccgcccggcc tgccgtccac cgcgcgcgac ctcaccctgg acgacgcgga cgcgctcgcc
      361 cgcctgcccc atgtgcgcca cgtcaccggt tcggtgagcg gcaacgccga ggtgcgcgcg
      421 caaggccggg tgcggcgcag caccgtgctc ggcgtgggac cgcagatgca ggaggtgtac
      481 tcgatgcggg tgcgcgtggg gcagttcctg ccgcccgacg aggcggcgag cgcgcgcgcc
      541 ttcgtggtgc tgggccccaa gctcgcacgc gagctcttcg gcagcaccat cgcgctcggc
      601 gagcacgtcg agatcggtgc cgagcgcttc cgcgtggtcg gcgtcatgga agagaagggg
      661 cagttcctcg gtatcgatct ggacgacgcc gcctacatcc cggtggtgcg cggcatggcg
      721 ctgtaccagc gcgacggcct catggagatc gcgttgacct acgaccccga ggcccccgcc
      781 gcgcgcgtgg cggaggcggt gaagaagcgg atgatcgcgc gccacggacg cgaggacttc
      841 accgtgctga cgcaggagga catgctggcg acgctgtcga acatcctcga tctgctcacc
      901 gcggcggtgg gtgcgctcgg ggcgatctcg ctgctggtgg gcggagtggg catcgtcacc
      961 atcatgagca tcgcggtcac cgagcgcacc ggagagatcg gcctgctggt ggccctgggc
     1021 gcacgtcggc gcaccatcct cgcgctcttc ctcggcgagg cggtggtgct ggcgggcatc
     1081 ggcggcctgc tcggcctgct cgtcggcgca ggccttgccc agctggtcgg gctgctggtg
     1141 ccggcgatgc cggtggccac gccctggcga tacgcgctcg ccgccgaggg ggtcgccatc
     1201 gtggtcgggc tcgccgccgg ggtcctgccc gcacgcaggg cggcacggct cgacgcggtc
     1261 gaggccttgc gcgcggagtg agccatgcgg cgcgcgcgcc cgttccagcg ccaccctcgc
     1321 acgcacgtcg ttcctgcaaa accaccgcgc ttccgggagc gcctggagcc aagcgagtcg
     1381 cgagtcggac gctgacgaag cgccgccccg ccctcagccg acgagccttt ccgcgcggat
     1441 cgcctcgtat tcgtcctgcc agaaccattt cgcctcgccg aaggcgggcg cgagctggtc
     1501 gagccaggtg gccttttcgt cgaaccgggc gaagaacggg cgttgcaccc agtcggggtt
     1561 gcggccctgg atgaagcgca gcacgaagac cttctcgccg gcgatctcgg tgacgccctg
     1621 gatctcgacc ttgccggggc tggcactcat gctcgggccg cgggcggtgc gggcgaggcc
     1681 ggagacctgc tggatggcct gctggtagat ctcccaggcg cgcgcgagcg gaacctcgaa
     1741 gtaatggcgg gcgccggtgt cgcgctcgac gaacatgtaa taggggacga ggccgaggcg
     1801 gacctcggtc ttccacaagc gcgcccaggc ggcggggtcg tcgttgatgt gggcgatgag
     1861 cggaccttgt gcgcggatga cggcaccggt ggcgcggatg cggcggatgg cggcgtgggc
     1921 ggcgtcggtc tcgagctcct tccagtggtt gtagtgcgcc atcagcgcga cgtgcttgcc
     1981 ggcttcgacg agctgccgga gcaggcgcat gagatcgtcg gcatcctcgg cgccgaggaa
     2041 gcggtgcggc cagaaggtga gcgccttgga gccgatgcgg atggtctgga tgtggtcgaa
     2101 ctcggggcgc agcaggggtt cgaggtattc gcgcaggtga cgggtcttca tgaccatggg
     2161 gtcgccaccg gtgacgagca ggtcggtgac ctcggtatgg gtgcgcaggt agtcgtgcag
     2221 cacgcgggct tcggagctgg cgatgcgcag ctccttgtcg ccgacgaatt gcgcccagcg
     2281 gaagcagaag ctgcaatagg cgtggcaggt ctggccctgg ctggggaaga agagcacggt
     2341 ctcgcgatac ttgtgctgca ggccgtcgat gcgcttgccg tgctcgtcga gcggcatgtt
     2401 catctgcatc tggtcggcgg ggtgcgggtt gagggcgtgg cggacctcgg cgatggcggc
     2461 ctcgagcgcg tccttgtccg cgtcgccttc gatgaggcgg gcgatgcgct cgtagtgctc
     2521 ggacgcgagc atgccgcgct gcgggaaggt gagctggaag atggggtcgc gggggacgtc
     2581 ggcccagtcg atgagctcgt cgatgacgta ttggttgacg cggaagggca gcacggcggc
     2641 cacgaccttc atctcgaagc gctgctcggg agaaaggcgc gcgagctgcg ggatgcggtc
     2701 gaggtcgcgg gcggtgaaga ccttgaaggg catcggggcg aagcgctcgg cggcgagcgc
     2761 ggcgcgcggg ttcggcactg cggcggcgat ggggaccg
//
LOCUS       DAOQNN010000420         2776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_26997_length_2776_cov_10.472620, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000420 DAOQNN010000000
VERSION     DAOQNN010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26997_length_2776_cov_10.472620"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKV97_15520"
     CDS             <1..249
                     /locus_tag="PKV97_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB07343.1"
                     /translation="PVASASAGIFLQLGAFSSFANAEGFRDTVRSQASDLAERFELFA
                     DGERFRLHAGPYDTVEAARDAAERMGNVLKLKPFVVVR"
     gene            358..1485
                     /locus_tag="PKV97_15525"
     CDS             358..1485
                     /locus_tag="PKV97_15525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNB07344.1"
                     /translation="MRFLIAVLVSLFSLSALAQTVPPPALAAKAWALVDHATGQTLAE
                     KDADARIEPASLTKLMTAYITFSKLKEGSLKPDQVVPVSTKAWRMEGSRMFIEPNKPV
                     SVDELIRGVIVQSGNDACVALAETIAGSEEAFAALMNREAQRLGMTNTHFTNSTGLPD
                     PQLYTSARDLAVLASAIVRDFPEYYGLYSLKEFTYNGIKQPNRNRLLYMDPSVDGMKT
                     GHTSSAGYCLVSTARRGERRLVSVVLGAASDSVRAQESLKLLNFGYQFYETVKLYSAD
                     EALSKFRVWKGKLNELPVGFTKDFALTVPKEAANKIQVSLESRQPLVAPLQRGQEVGT
                     LTVAVDGKPLGQYPVVALQDVEVAGFFGRLWDAIVMFFKSL"
     gene            1528..1812
                     /locus_tag="PKV97_15530"
     CDS             1528..1812
                     /locus_tag="PKV97_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HNB07345.1"
                     /translation="MADQQDAQTRQSLIEYPCDFPIKVMGARVDGFAQAVIDVVLRHA
                     PDFDPAGAEMRPSSKGNYLAVTCTFRALSQVQVDAMYRELTAHPMVKVVL"
     gene            1809..2477
                     /gene="lipB"
                     /locus_tag="PKV97_15535"
     CDS             1809..2477
                     /gene="lipB"
                     /locus_tag="PKV97_15535"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNB07346.1"
                     /translation="MIVKRLGLVEYAPALEAMRVFTAARGADTSDEIWLLQHPPVYTL
                     GQAGKPEHLLQNPADIPLVRIDRGGQITYHGPGQLVAYLLLDLPRRRLKVRELVHLME
                     QAIIDTLADYGLAAQRKDGAPGVYIAGDKIAALGLRVRNGCSYHGLAINVDADLAPFG
                     WINPCGYEGLKTIRMKDFGIDASVDAVGERLLGHLQALLPPIGTADAADQPPGAAASA
                     RAGP"
     gene            2600..>2776
                     /locus_tag="PKV97_15540"
     CDS             2600..>2776
                     /locus_tag="PKV97_15540"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584312.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNB07347.1"
                     /translation="METPARKQRGADKTARIPIKIVPAERLKKPDWIRIKLGAGQEAE
                     RFNEIKATLRDHKLH"
BASE COUNT          459 a         1011 c          876 g          430 t
ORIGIN      
        1 ccggtcgcgt cggcgagcgc gggcatcttc ctgcagctcg gcgccttctc ctcgttcgcc
       61 aacgccgaag gcttccgcga cacggtgcgc agccaggcct cggatctggc cgagcgtttc
      121 gaattgttcg ccgatggcga acgtttccgc ctccacgccg gtccctacga cacggtcgag
      181 gcggcgcgcg atgcggccga gcgcatgggc aacgtgctca agctcaagcc cttcgtggtg
      241 gtgcgctgac cgtcgtgcac cgcgcccggg ttgcaacaaa ggctttccat tttttgcccg
      301 tccatgcgcg gcgcctataa tccgccgttc ccattcccgc ttctccaggt tttcccgatg
      361 cgtttcctca ttgccgtact cgtctcgctg ttctcgctgt ccgcgctcgc gcagacggtg
      421 ccgccccccg cgctggccgc caaggcctgg gcgctggtcg accatgccac cgggcagacg
      481 ctcgccgaga aggacgccga cgcccgcatc gagccggcct cgctgaccaa gctgatgacg
      541 gcctacatca ccttctccaa gctcaaggaa ggctcgctca agcccgacca ggtggtgccg
      601 gtctccacca aggcctggcg catggaaggc tcgcgcatgt tcatcgagcc caacaagccg
      661 gtcagtgtgg atgagctcat ccgcggcgtg atcgtgcagt cgggcaacga cgcctgcgtg
      721 gcgctcgccg agaccatcgc cggcagcgag gaagccttcg ccgcgctgat gaaccgcgag
      781 gcgcaacgcc tgggcatgac caacacccac ttcaccaatt ccaccggcct gcccgatccg
      841 cagctgtaca ccagtgcgcg cgacctcgcc gtgctggcga gcgcgatcgt ccgcgacttc
      901 cccgagtact acggcctgta ttcactcaag gaattcacct acaacggcat caagcagccc
      961 aaccgcaacc gcctgctgta catggacccc tccgtcgacg gcatgaagac cggccacacc
     1021 agcagcgccg gctactgcct cgtgtccacc gcccgtcgcg gcgagcgccg cctggtctcg
     1081 gtggtgctgg gtgcggcctc ggactccgtg cgcgcgcagg agtcgctcaa actgctcaac
     1141 ttcggctacc agttctacga gacggtcaag ctctactccg ccgacgaggc gctgtcgaag
     1201 ttccgtgtct ggaagggcaa gctcaacgag ctgccggtcg ggttcacgaa ggacttcgcg
     1261 ctcaccgtgc ccaaggaggc ggcaaacaag atccaggtca gcctggagag ccgccagccc
     1321 ctggtggcgc cgctgcagcg cggccaggag gtcggcaccc tgacggtcgc ggtcgacggc
     1381 aagccgctcg gccagtaccc ggtggtggcg ctgcaggacg tcgaggtggc gggcttcttc
     1441 ggccgcctgt gggatgcgat cgtgatgttc ttcaagagct tgtgatgcgc gctccggacc
     1501 gcgaatccac gaatacggtg atccgccatg gctgaccagc aggacgcgca gacgcgccag
     1561 agcctgatcg agtatccctg cgactttccc atcaaggtga tgggcgcgcg ggtggacggc
     1621 ttcgcccagg cggtgatcga cgtggtgctg cgccacgcgc ccgacttcga tcctgccggc
     1681 gccgagatgc ggccctcgag caagggcaat tacctcgcgg tgacctgcac cttccgcgcg
     1741 ctgtcgcagg tgcaggtgga cgcgatgtac cgcgagctca ccgcgcaccc gatggtcaag
     1801 gtggtgctgt gatcgtcaag cgcctcggcc tggtggagta cgcgccggcg ctcgaggcga
     1861 tgcgcgtgtt caccgccgcg cgcggcgccg acacgtccga cgagatctgg ctgctgcagc
     1921 acccgccggt gtacaccctc ggccaggccg gcaagcccga gcacctgctg cagaacccgg
     1981 ccgatattcc gctggtgcgc atcgaccgtg gcggccagat cacctatcac ggccccggtc
     2041 agctggtggc ctacctgctg ctggacctgc cgcggcgcag gctcaaggtg cgcgagctgg
     2101 tgcacctgat ggagcaggcg atcatcgaca ccctggccga ttacggcctc gccgcacaac
     2161 gcaaggatgg cgcgccgggg gtctacatcg ccggcgacaa gatcgccgcg ctcggcctgc
     2221 gcgtccgcaa cggctgcagc tatcacggcc tggcgatcaa cgtggatgcc gacctcgcgc
     2281 ccttcggctg gatcaacccc tgtggctacg aggggctgaa gacgatccgc atgaaggatt
     2341 tcggcatcga tgccagcgtg gacgcggtcg gcgagcgtct gctcggccac ctgcaggccc
     2401 tgctgccgcc catcggcacc gccgatgcgg cggatcagcc gccgggggct gccgcgagcg
     2461 cacgcgccgg gccataatcc gctcaccggc gccgcagagc gccagcgccc ggcccgatcg
     2521 ctcgcgcacc ccgagcgcat ggcgcccacc ctgactccag cccccttccc cacccggaaa
     2581 cccacagaga cccacgaaca tggaaacccc cgctcgcaag cagcgcggcg ccgacaagac
     2641 cgcccgcatc ccgatcaaga tcgtccccgc cgaacgcctc aagaagcccg actggatccg
     2701 catcaagctc ggcgccggcc aggaggccga gcgcttcaac gagatcaagg ccacgctgcg
     2761 cgaccacaag ctgcac
//
LOCUS       DAOQNN010000421         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_27011_length_2775_cov_9.272426, whole genome shotgun sequence.
ACCESSION   DAOQNN010000421 DAOQNN010000000
VERSION     DAOQNN010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27011_length_2775_cov_9.272426"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKV97_15545"
     CDS             <1..794
                     /locus_tag="PKV97_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNB07348.1"
                     /translation="AFDADTLELVADVPATTEDGIRSKVVGLADLSGKRFIYSLFEAG
                     EIRITDFSDPKNPLTRRFAGGKQPYDALVTPDGRYYIAGLFGEDGLALIDLWNLDKGS
                     RKILSGYGRGEQPLPVFKMPHLRGWSIAGNRAYLPAIGRHEVLVVDIATWQEVDRIQV
                     KSQPVFAMARPDGREIWVNFAFPDNGWVQVIDTVTGKVTDTLQPGRGILHMEFLSKGH
                     EIWLSARDDNKVVIYDTASKQPIGGFESASPSGIFFTTRAARTGF"
     gene            794..1843
                     /locus_tag="PKV97_15550"
     CDS             794..1843
                     /locus_tag="PKV97_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNB07349.1"
                     /translation="MPRQAILPAALDATGFRLLNEWQRDFPLEPRPFARIAQAVGAAE
                     EDVISAYRDLSAQGLVSRVGAVFAPRRLGASALAALAAPPERLEEVAARVTREPAINH
                     NYQREHRYNLWFVVTAASRAQLEEVVAGIERDTGCAVIVLPLEEEFHIDLGFDLKAGG
                     EGRRPHADARSDEPIARGDAEGGCALPALERKLIGALQGGLVLDARPFATLAARVGLT
                     EGEVIACIARWVDEGLIRRFGVVVRHHELGLEANAMCVWDVPDAEVSGLGRRLAQEAA
                     VTLCYRRRRVLPGWPYNLFCMIHGSAREEVLAARADLAARLGLDRHPHAVLFSCRRFK
                     QTGARYLDAKDLAHV"
     gene            1836..2339
                     /locus_tag="PKV97_15555"
     CDS             1836..2339
                     /locus_tag="PKV97_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HNB07350.1"
                     /translation="MSEAQPAASVGRRGVAAKRAPDDTDRRLINAIQGEFPLSERPFA
                     EVGVLLGLAEDEVIARLQSLLDDRVLTRFGPMYQIERMGGAFCLAAIAVPEAQWTSAV
                     EAVNAFPEVAHNYRREHALNMWFVLATETPEGIADCARRIEAATGLVVHLFPKEREYF
                     VEMRLEA"
     gene            2408..>2775
                     /locus_tag="PKV97_15560"
     CDS             2408..>2775
                     /locus_tag="PKV97_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNB07351.1"
                     /translation="MDEAALAEFDRRLVLATQGGLPLVPRPYAALAETLGVAEGRVRE
                     RLAAMLADGRIRRIGAVPNHYAIGYTANGMSVWDIDAAVIDAVGERVGALDFVTHCYR
                     RPRHLPDWPYNLFAMVHAAS"
BASE COUNT          395 a          996 c          957 g          427 t
ORIGIN      
        1 aggccttcga cgccgacacg ctcgaactcg tcgccgatgt gcctgcaacc accgaggacg
       61 gcatccgctc caaggtcgtt ggcctggccg acctctccgg caagcgcttc atctattcgc
      121 tgttcgaggc gggcgagatt cgcatcaccg acttctccga cccgaagaat ccgctgaccc
      181 ggcgttttgc cggcggcaag cagccctacg atgcactggt cacgcccgat ggccggtact
      241 acatcgccgg cctgttcggc gaggacggcc ttgccctgat cgacctgtgg aacctcgaca
      301 agggcagccg caagatcctc tccggctacg gtcgtggcga gcagcccctg ccggtgttca
      361 agatgcccca cctgcgcggc tggtcgatcg ccggcaatcg ggcctacctg cccgccatcg
      421 gtcgccacga ggtgctggtg gtcgacatcg ccacctggca ggaggtcgat cgcatccagg
      481 tgaagagcca gccggtgttc gcgatggcgc gtccggacgg gcgcgagatc tgggtgaact
      541 tcgccttccc cgacaacggc tgggtgcagg tcatcgacac cgtcaccggc aaggttaccg
      601 acaccctgca gccgggccgc ggcatcctgc acatggagtt cctctccaag ggccacgaga
      661 tctggctgtc ggcgcgcgac gacaacaagg tcgtgatcta cgacaccgcc agcaagcagc
      721 cgatcggcgg cttcgagtcg gcgagcccga gcggcatctt cttcaccacg cgcgccgcgc
      781 gcacgggctt ctgatgccgc gccaggccat cctgcccgcg gcgctcgacg ccaccggctt
      841 ccgcctgctc aacgagtggc agcgtgactt cccgctcgag ccgcgacctt tcgcacgcat
      901 cgcgcaggcg gtgggcgccg ccgaggaaga cgtcatctcg gcctaccgcg acctgagcgc
      961 gcaaggcctg gtgagccggg tgggcgcggt gttcgctccg cgtcgcctgg gtgcgagcgc
     1021 actcgcggcc ctggccgcgc cgccggagcg cctggaggag gtcgccgcgc gcgtcacgcg
     1081 tgagccggcg atcaaccaca actaccagcg cgagcatcgc tacaacctct ggttcgtggt
     1141 caccgcggcc tcgcgtgcgc aactcgaaga ggtggtcgcc ggcatcgagc gcgacaccgg
     1201 ctgtgcggtc atcgtgctgc cgctggaaga agagttccac atcgacctgg gcttcgacct
     1261 caaggcgggc ggggaggggc ggcgaccgca tgccgacgcg cgcagcgacg agccgatcgc
     1321 acggggcgac gccgagggcg gctgcgcgtt gcctgcgcta gaacgcaagc tgatcggcgc
     1381 gctgcagggc ggcctggtgc tggacgcgcg ccccttcgcc acgctggcgg cgcgcgtcgg
     1441 cctcaccgag ggcgaggtga tcgcctgcat cgcgcgctgg gtggacgagg gcttgatccg
     1501 ccgcttcggc gtggtcgtgc gccaccatga gctcggcctc gaggcgaacg ccatgtgcgt
     1561 gtgggacgtc cccgacgccg aggtctccgg gcttggtcgc cggctcgcgc aggaggcggc
     1621 ggtcacgctg tgctaccgcc gccgccgcgt gctgcccggc tggccgtaca acctgttctg
     1681 catgatccac ggcagcgcgc gcgaggaggt gctggccgcg cgggcggatc tcgccgcgcg
     1741 cctcggcctc gaccgccatc cgcacgcggt gctgttcagt tgtcggcgtt tcaagcagac
     1801 cggcgcgcgc tatctcgacg ccaaggatct cgcccatgtc tgaagcccag ccggccgcgt
     1861 cggtcggccg tcgtggcgtg gccgcgaagc gtgcgcccga cgacaccgac cgtcgcctga
     1921 tcaatgccat ccagggcgag ttcccgctct ccgagcgccc ctttgccgag gtcggcgtcc
     1981 tgctcggcct ggcggaggac gaggtgatcg cgcgcctgca gtcgctgctc gacgaccgcg
     2041 tgctgacccg tttcgggccg atgtaccaga tcgagcgtat gggcggcgcc ttctgcctgg
     2101 ccgcgatcgc ggtgcccgag gcgcagtgga cgagtgcggt ggaggcggtc aatgccttcc
     2161 ccgaggtcgc gcacaactac cgccgcgagc acgcgctcaa catgtggttc gtgctcgcca
     2221 ccgagacgcc ggagggcatc gccgactgcg cgcgccgcat cgaggccgct accggcttgg
     2281 tggtgcatct gttccccaag gaacgcgagt acttcgtcga gatgaggctg gaggcctgat
     2341 ggtgcaaggc atgtccggga acggagccct cgcgcgggcg gatgcgcagg atgccgcggg
     2401 tgccggcatg gatgaagctg ccctcgcgga gttcgaccgc cgcctcgtgc tcgccaccca
     2461 gggcggcctg ccgctggttc cacgccccta cgccgcgctg gccgagacgc tcggcgtggc
     2521 cgagggccgc gtgcgcgagc gcctcgccgc catgctcgcc gacggccgca ttcgccgcat
     2581 cggcgcggtg cccaaccact acgccatcgg ctacaccgcc aatggcatga gcgtgtggga
     2641 catcgacgcc gcagtgatcg acgcggtcgg cgagcgcgtc ggtgcgctcg acttcgtcac
     2701 ccactgctac cgccgcccgc gccatctgcc cgactggccc tacaacctgt tcgcgatggt
     2761 gcacgccgcc agcca
//
LOCUS       DAOQNN010000422         2752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_27438_length_2752_cov_9.019281, whole genome shotgun sequence.
ACCESSION   DAOQNN010000422 DAOQNN010000000
VERSION     DAOQNN010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27438_length_2752_cov_9.019281"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /gene="cobO"
                     /locus_tag="PKV97_15565"
     CDS             <1..599
                     /gene="cobO"
                     /locus_tag="PKV97_15565"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNB07352.1"
                     /translation="PDRDTRHNARMARKKDVVDEKIAAAQAERGVLLVNTGNGKGKSS
                     AGFGLVARALGHGMQVGVVQFIKGRSDTGEEAFFRTQPGVRWHVMGEGFTWETQDRAR
                     DVASAEAAWALAREMLQNPEVGLVVLDELNIALKYQYLDVERVLADLRARPPMQHVVV
                     TGRGAPPALVEAADTVTDMTPVKHAFAAGVKAMPGMEW"
     gene            602..1450
                     /gene="cobA"
                     /locus_tag="PKV97_15570"
     CDS             602..1450
                     /gene="cobA"
                     /locus_tag="PKV97_15570"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNB07353.1"
                     /translation="MAVPDLAVHRPGPLPGWVYLVGAGPGDPELFTLRGARLVAGADV
                     IVYDNLVAPPILDLAPVEAERIYVGKKAADHSLPQDEINQLLLELARRNKRVVRLKGG
                     DPFIFGRGGEEMELLVEAGIHVEVVPGVTAAAGVSAYAGIPLTHREHAQSVVFATGFL
                     KEGKVDLDWPMLARPHQTVVIYMGISRLADICAQLVAHGLPPTTPAGVIERGTTHAQR
                     VVVADLATLAQRVLDEGVRPPSLTVVGSVVGLYPKLAWFEPQEQPKASPLPHAGCAVV
                     HGKGGE"
     gene            1465..>2752
                     /locus_tag="PKV97_15575"
     CDS             1465..>2752
                     /locus_tag="PKV97_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HNB07354.1"
                     /translation="MPETVDPGAASCPALFVAAPASGQGKTTVTAALARLHTRLGRRV
                     RVFKCGPDFLDPQIHAVASGAPVHNVDLGMCGEADIARRLHAAAREADLILVEGVMGL
                     YDGAPSGADIARRFGIPVMAVIDARAMAQTFGAVAYGLAHYQPGLPFAGVLANHVGSA
                     RHADMLRESLPAGMRWFGAVMRDVDAALPERHLGLLQAAEIDDLGARLDRLADHLERT
                     GAAELPPTVAFPAVAVAEIPPLLAGKRIAIAHDAAYGFLYPANVDTLRALGAELAFFS
                     PLAGEGLPAQCDAVWLPGGYPELHGAALAANAGLWVGLRAHVAAGKPVLAECGGLMSL
                     LDAIVDKVGVEHAFAGLLPGRAVMQKRLAALGMQEVDLPGGRLTGHTFHYSRTETPLV
                     PWLRARRPDGGEGEAIYRRGPITASYVHFWFPSNPVA"
BASE COUNT          366 a          958 c         1015 g          413 t
ORIGIN      
        1 cccccgaccg cgacacccgc cacaacgccc gcatggcacg caagaaggac gtcgtcgatg
       61 aaaagatcgc cgccgcgcag gccgagcgcg gcgtgctgct ggtcaacacc ggcaacggca
      121 agggcaagtc gagcgcgggt ttcggcctgg tggcgcgcgc gctcgggcac ggcatgcagg
      181 tcggcgtggt gcagttcatc aaggggcgct cggacaccgg cgaggaggcc ttcttccgca
      241 cgcagcccgg cgtgcgctgg cacgtgatgg gcgagggctt cacctgggag acccaggatc
      301 gtgcccgcga cgtcgcgtcg gccgaagccg cctgggcgct ggcacgcgaa atgcttcaga
      361 atcccgaagt cggcctcgtg gtgctggacg agctcaatat cgcactcaag taccagtatc
      421 tcgacgtgga gcgggtgctc gccgacctgc gcgcccggcc gccgatgcag catgtggtgg
      481 tcaccggccg cggggcgccg cccgcgctgg tcgaggctgc cgacaccgtc accgacatga
      541 cgccggtcaa gcacgccttc gccgcggggg tgaaggcgat gcccggcatg gagtggtaag
      601 aatggcagtt cccgatctcg cggttcatcg tcccggcccg ctgccgggct gggtctatct
      661 ggtgggcgcc ggcccgggcg acccggagct gttcacgctg cgcggcgcgc gcctggtggc
      721 gggcgcggac gtcatcgtct acgacaacct cgtcgcgccg cccatcctgg atctggcgcc
      781 ggtcgaggcc gagcgcatct acgtcggcaa gaaggcggcc gaccactcgc tgcctcagga
      841 cgagatcaac cagctgctgc tcgagctggc gcggcggaac aagcgcgtgg tgcggctcaa
      901 gggcggcgac cctttcatct tcggccgtgg cggcgaggag atggagctgc tggtcgaggc
      961 cggcatccac gtcgaggtgg tgccgggcgt gaccgcggcg gcgggtgtct ccgcctacgc
     1021 ggggatcccg ctcactcatc gcgagcacgc gcagtcggtg gtgttcgcca ccggcttcct
     1081 caaggaaggc aaggtcgacc tcgactggcc gatgctggca cgtccgcatc agacggtggt
     1141 gatctacatg ggcatctcgc gcctggccga catctgcgcc cagctcgtcg cccacggcct
     1201 gccgccgacc acgcctgcgg gggtgatcga gcgcggcacc acccatgcgc agcgcgtggt
     1261 ggtggccgac ctcgccaccc tggcgcagcg cgtgctggac gaaggcgtgc gtcccccctc
     1321 gctgaccgtg gtcggcagcg tggtggggct gtatcccaag ctggcctggt tcgagccgca
     1381 ggagcagccc aaggcctcgc cgctgccgca cgccggctgt gcggtggtgc acggaaaggg
     1441 tggcgagtga gcgtggcgga cgcgatgccg gaaacggtcg acccgggtgc ggcctcgtgc
     1501 ccggcactct tcgtcgccgc acccgcctcc ggccagggca agaccaccgt caccgcggcg
     1561 ctcgcgcgcc tgcacacgcg gctgggacgg cgggtgcggg tgttcaagtg cgggccggac
     1621 ttcctcgacc cgcagatcca cgcggtggcg agcggcgcgc cggtgcataa cgtcgacctg
     1681 gggatgtgcg gcgaggcgga catcgccagg cggctgcacg ccgcggcgcg cgaggccgac
     1741 ctgatcctgg tcgaaggtgt gatgggcctc tacgacggcg cgccctcggg cgccgacatc
     1801 gcacgccgct tcggcatccc ggtgatggcg gtgatcgacg cacgcgcgat ggcgcagacc
     1861 ttcggcgcgg tggcttacgg gttggcgcat taccagcccg ggttgccctt tgccggggtg
     1921 ctcgccaacc acgttggcag cgcgcgccac gccgacatgc tgcgcgagtc gctgccggcg
     1981 ggcatgcgct ggttcggtgc ggtgatgcgc gatgtggatg cggcgctgcc cgagcgtcat
     2041 ctcggtctgc tgcaggcggc ggagatcgac gacctcggtg cgcgtctcga ccgcctcgcc
     2101 gatcatctcg agcgcaccgg cgccgcggag ctgccgccga cggtggcgtt tcccgccgtg
     2161 gcagtggcgg agattccgcc actgctcgcc ggcaagcgga tcgcgatcgc gcacgacgcc
     2221 gcttacggct tcctctaccc ggccaatgtc gacaccctgc gcgcgctggg tgcggagctc
     2281 gccttcttct cgccgctcgc cggcgagggc ttgccggcac agtgcgacgc cgtctggctg
     2341 ccgggcggct accccgagct gcacggcgcc gcgctggccg ccaacgccgg gttgtgggtc
     2401 gggctgcgag cgcatgtggc cgccggcaag ccggtgctcg ccgagtgcgg gggcctgatg
     2461 agcctgctcg atgccatcgt cgacaaggtc ggggtggagc acgcgttcgc cggcctgctg
     2521 cccggccggg cggtgatgca gaaaaggctc gccgcgctcg gcatgcagga agtcgatctg
     2581 cccggcggtc ggctcaccgg ccacaccttt cattattcgc gcacggagac cccgctggtg
     2641 ccctggctgc gcgcgcgccg ccccgacggc ggcgagggcg aggcgatcta ccgtcgcggc
     2701 ccgatcaccg cctcgtacgt gcatttctgg ttcccgtcga atccggtggc gg
//
LOCUS       DAOQNN010000423         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_27542_length_2745_cov_7.084387, whole genome shotgun sequence.
ACCESSION   DAOQNN010000423 DAOQNN010000000
VERSION     DAOQNN010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27542_length_2745_cov_7.084387"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="PKV97_15580"
     CDS             <1..1308
                     /locus_tag="PKV97_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07355.1"
                     /translation="DGRRIVLERGELALDGVAAASARGVFDTFAGTLDTLAVELTDVD
                     LVRIGPRWLAPLLAPASAQRLRLEGRADLRAELRRDRLETLDVELHGAGFGLASPQGE
                     GIAAGGVAEEARAEVLAFGPVDGRVRWSSRGRGEAALQVGGGRWEKLALGAFALDAVL
                     GPAGLRLPGARIPLLDGALVLRDLALGWSVAGWEGSAGAVLEPVSMPLLTEALGLPRM
                     AGVMSAALPGLRLRPGELVLDGTLAVSVFGGWVQASGLRVHEPFGVASHLTGEIEARH
                     IDLAQLTEAFSFGSITGHIDADVRGLELSSWRPTAFDARIASIAGDERRRISQRAVQN
                     LGALGGGGALAAVQRSVLRLFETFGYRELGLRCRLAKGVCEMDGLDAAPDGAARADGG
                     FTIVRGGGVPALDVIGYNRRVDWNELVARLQRVVAEGVSPTID"
     gene            1333..1527
                     /locus_tag="PKV97_15585"
     CDS             1333..1527
                     /locus_tag="PKV97_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07356.1"
                     /translation="MNPVARWLFAAALVASTAACVTINIYFPAAAAEKAADRIIDEVW
                     QLREGAGSAPATPPAAGDAK"
     gene            1524..1964
                     /locus_tag="PKV97_15590"
     CDS             1524..1964
                     /locus_tag="PKV97_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HNB07357.1"
                     /translation="MKSASMPSGSWAGRLAALFVGLALAFPVHAQGNLEIDTPAISAL
                     RKTMQQRHAQLAPLYASGAVGIAADGTVSLRDAAAVPLAQRAQANAAVAAENADRAAL
                     YREIARANGHPEWEAEVRRTFAQRWLERAQPGWWVQQGAAWSRK"
     gene            1961..>2745
                     /locus_tag="PKV97_15595"
     CDS             1961..>2745
                     /locus_tag="PKV97_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB07358.1"
                     /translation="MMPVLVFDIETIPDVAGIRAIENLPAGLSDYEVAEWAFQQRRAS
                     HGNDFLPHHLQRVVAISCVLRDAQHFRVFSLSEPESGEGEIIQRFFDGIERYSPQIVS
                     WNGGGFDLPVLHYRGMLHGVAAPRYWDQGEGDYHDSRDFKWNNYISRYHSRHLDLMDL
                     LALYQPRASAPLDDLAKLMGYPGKLGMDGSAVWQAWQEGRIAEIRDYCETDVVNTYLV
                     YLRFERMRGHLDAAAWAAEVETVREALGRIDAPHWKQFLAAWP"
BASE COUNT          351 a          957 c         1014 g          423 t
ORIGIN      
        1 gacggccgcc ggatcgtgct cgagcgcggc gagctggcgc tcgatggggt tgcagccgcc
       61 tccgcgcgcg gcgtgttcga caccttcgcc ggcacgctgg acaccctcgc ggtcgagctg
      121 accgatgtcg atctcgtccg catcggcccg cgctggctgg cgccgctgct ggcgccggcg
      181 agtgcgcagc ggttgcgcct cgaggggcgg gcggacctgc gtgccgaatt gcgccgggat
      241 cggctggaga cgctcgacgt ggaactgcat ggggcgggct tcggtctcgc gtcgccgcag
      301 ggcgagggaa tcgccgcagg cggcgtagcg gaggaggcgc gcgccgaggt gctcgccttc
      361 ggccccgtgg atgggcgcgt gcgctggtcc tcgcgcggcc ggggcgaggc cgccctgcag
      421 gttggcggag ggcgctggga gaagctcgcg ctcggggcct tcgcgctcga cgcggtgctc
      481 gggcccgccg ggctgcgcct gccgggcgcg cgcatcccct tgctcgacgg tgcgctggtg
      541 ctccgggatc tcgccctcgg ctggtccgtc gcaggctggg aggggagcgc tggcgcggtc
      601 ctcgagccgg tgtcgatgcc cttgctcacc gaggcgctcg gtctgccgcg catggcgggg
      661 gtgatgtcgg ccgcgctgcc gggcttgcgc ctgcgtccgg gcgagctcgt gctcgacggc
      721 acgctcgcgg tgtccgtctt tggcgggtgg gtgcaggcga gcggactgcg cgtgcacgaa
      781 cccttcggcg tcgcttcgca cctgacgggc gagatcgagg cgcgccacat cgatctcgcc
      841 cagcttaccg aggccttcag cttcggcagc atcaccggtc atatcgacgc cgatgtgcgc
      901 ggcctggagc tgtcgagctg gcggcccacg gccttcgacg cccgcatcgc cagcatcgcc
      961 ggcgacgagc gccgccgcat cagccagcgc gcggtgcaga acctcggcgc gctcggcggc
     1021 gggggcgcgc tcgcggcggt gcagcgcagc gtgctgcgcc tgttcgagac tttcggctat
     1081 cgcgagctcg gcctgcgctg ccggcttgcc aagggcgtat gcgagatgga cgggctggat
     1141 gcggcgccgg acggcgccgc gcgcgccgat ggcggcttca ccatcgtgcg tggcggaggc
     1201 gtcccggcac tcgacgtcat cggctacaat cggcgcgtcg attggaacga actggtggcc
     1261 cgcctgcagc gcgtggtggc cgagggcgtc tctcccacga tcgattgaat ctgcatatcc
     1321 cgcgaggacg gcatgaaccc tgttgcgcgc tggctgttcg cggctgcgct ggttgcgagc
     1381 accgcggcct gcgtcacgat caacatctat ttcccggccg cggcggccga gaaggccgcc
     1441 gaccggatca tcgacgaggt ctggcagctg cgcgagggtg cgggcagcgc gcctgccacg
     1501 ccgccggccg cgggagacgc gaaatgaaat ccgcttcgat gccgtccggc tcctgggcgg
     1561 gccgcctggc ggccctgttc gtcggcctcg cgctcgcctt cccggtgcat gcgcagggca
     1621 acctcgagat cgacaccccg gcgatctccg cgctgcgcaa gacgatgcag cagcgccacg
     1681 cgcagctcgc gccgctgtat gcgtcgggtg cggtgggcat cgccgcagac ggtaccgtga
     1741 gcctgcgcga cgccgcggcg gtgccgctcg cgcagcgtgc ccaggccaac gccgcggttg
     1801 ccgccgaaaa tgccgaccgt gccgcgctct accgcgagat cgcgcgcgcc aacggtcacc
     1861 ccgagtggga ggccgaggtg cgccgcacct tcgcgcagcg ttggctcgag cgcgcgcagc
     1921 cgggctggtg ggtgcagcag ggcgctgcgt ggtcgaggaa atgatgccgg tcctcgtctt
     1981 cgacatcgaa accattcccg acgtcgccgg catccgcgcg atcgagaacc tgcccgcggg
     2041 cctgtccgac tacgaggtcg cggagtgggc gttccagcag cgccgcgcca gccacggcaa
     2101 cgacttcctg ccgcaccacc tgcagcgcgt ggtggcgatt tcctgtgtgc tgcgcgacgc
     2161 gcaacacttt cgggtgttct cgctgtccga gcccgaaagc ggcgagggcg agatcatcca
     2221 gcgcttcttc gacggcatcg agcgctactc gccacagatc gtgtcgtgga acggcggcgg
     2281 cttcgacctg ccggtgctgc actaccgcgg catgctgcac ggcgttgcgg caccgcgcta
     2341 ctgggaccag ggcgagggcg actaccacga ctcgcgcgac ttcaagtgga acaactacat
     2401 cagccgctac catagtcgtc atctggatct gatggacttg ctggcgctct accagccgcg
     2461 cgcgagcgcg ccgctcgacg acctcgccaa gctcatgggc tatcccggca agctcggcat
     2521 ggacggttcg gcggtctggc aggcctggca ggagggccgg atcgccgaga tccgcgacta
     2581 ctgcgagacc gacgtcgtca acacctacct cgtctacctg cgcttcgagc gcatgcgcgg
     2641 gcatctcgac gccgccgcgt gggcggcgga ggtggagacg gtgcgcgagg cgctcggccg
     2701 catcgacgca ccgcactgga agcagttcct cgccgcctgg ccgca
//
LOCUS       DAOQNN010000424         2739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_27647_length_2739_cov_9.849106, whole genome shotgun sequence.
ACCESSION   DAOQNN010000424 DAOQNN010000000
VERSION     DAOQNN010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27647_length_2739_cov_9.849106"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="PKV97_15600"
     CDS             complement(<1..1315)
                     /locus_tag="PKV97_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB07359.1"
                     /translation="MSLPLPRSLFARLMLIWLVGMALVLAVSLALFLGERERIGRSAL
                     FEGVAQEIASVAELLDRMSPPERERWIDEIGRRRLRLALRPPHDHLRPLADDHPLQAA
                     LREAMPQRPATLYTHPRDDRPHPGLAIVLALADGTPLQVRLPGIPPLPPIMPHQRGPF
                     LAALAALVLGVGLLTWIAVRIATRPLSRMAEAARALGEDPTRAPMDTRGPTEVAQAAA
                     AFNQMQQRVAEHVAERTRILAAISHDLQTPITRLRLRAEMVDDEGLRARIQSDLDAMQ
                     GLVKEGLAYARSLDERSPAQAIELGPLLQALAEDARDMGWTVRVPEGAQGRIVGNPSA
                     LRRALWNLIENAVKFGGKVEVALACVGEHCEIRVRDHGPGLAEDELEKVFEPFYRTEA
                     SRNRETGGTGLGLAITRNLLHAQGGEVTLRNHPDGGLEAVVRLRVP"
     gene            complement(1312..2082)
                     /locus_tag="PKV97_15605"
     CDS             complement(1312..2082)
                     /locus_tag="PKV97_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB07360.1"
                     /translation="MNTSDHILIVDDDRDIRQLLADYLEKQGLRCTTAADGREMKAAL
                     ETHRIDLIVLDLMMPGEDGLTLCRNLRASGGPHANTPILMLTARGEDMDRIIGLEMGA
                     DDYLAKPFVPRELFARIRAVLRRTRALPPNLDAAPPANARLLRFAHWRLDTINRHLVD
                     AEGTVVALSGAEYRMLAVFLSHPQKVLSRDQLMELTQGREADVFDRSIDLLVSRLRQR
                     LGDNAREPAIIKTVRNEGYVLAAEVVAETADTDGSGAR"
     gene            2279..>2739
                     /locus_tag="PKV97_15610"
     CDS             2279..>2739
                     /locus_tag="PKV97_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNB07361.1"
                     /translation="MKNWIKTSIAAAIIATGAIGSTAALAWGGHCDGPRGGKAGWSQM
                     APEQMKAKMAARAELHMARLELALALAPEQKPAFERFKADMKARAERMGSMMAERRAA
                     EPPKTAIERMQRMEEMSKLRQAEMGEARKSIETFYATLSDAQKTVFDAEFE"
BASE COUNT          401 a          908 c         1044 g          386 t
ORIGIN      
        1 acggcacgcg cagccgcacg accgcctcca gcccgccgtc ggggtgattg cgcagcgtga
       61 cctcgccgcc ctgggcatgc agcaggttgc gcgtgatcgc cagcccgagc ccggtcccac
      121 cggtctcgcg gttgcgcgag gcttcggtgc ggtagaaggg ctcgaacacc ttctcgagct
      181 cgtcctccgc gaggccgggg ccgtggtcgc ggacgcggat ctcgcagtgc tcgcccacgc
      241 aggccagcgc cacctcgacc ttgccgccga acttcactgc gttctcgatg aggttccaca
      301 gcgcgcggcg cagcgccgag gggttgccga cgatgcggcc ctgcgctccc tcgggcacgc
      361 gcacggtcca gcccatgtcg cgcgcatcct cggcgagggc ctgcagcagg gggccgagct
      421 cgatcgcctg cgccggactg cgttcgtcca ggctgcgcgc gtaggcgagg ccttccttca
      481 ccagtccctg catggcgtcg aggtcggact ggatgcgcgc gcgcaggccc tcgtcgtcga
      541 ccatttcggc gcgcaggcgc aggcgggtga tcggcgtctg caggtcgtgg gagatcgctg
      601 ccaggatgcg ggtacgctcg gcgacgtgct cggcgacacg ctgctgcatc tggttgaagg
      661 cggcggcggc ctgggcgacc tcggtggggc cgcgcgtgtc catgggtgcg cgcgtcgggt
      721 cctcgccgag cgcgcgcgcc gcctccgcca tgcgcgacag cggtcgggtg gcgatgcgca
      781 ccgcgatcca ggtgagcaag cctaccccca gcaccagcgc ggcgagggcg gcgaggaagg
      841 ggccgcgctg atgcggcatg atcggcggca acggcgggat gcccggcagg cggacctgca
      901 gcggggtgcc gtcggcgagc gcgagcacga tcgccaggcc ggggtggggg cggtcgtcgc
      961 gcggatgggt gtacagcgtg gccgggcgtt gcggcatggc ctcgcgcagc gcggcctgca
     1021 gcgggtggtc gtcggcgagc ggacgcagat ggtcgtgggg ggggcgcagc gcgaggcgca
     1081 ggcggcggcg gccgatctcg tcgatccagc gctcgcgctc gggcggcgac atgcggtcga
     1141 gcagctcggc gacgctggcg atctcctgtg ccacgccctc gaacagcgca ctgcgtccga
     1201 tgcgctcgcg ctcgccgagg aagagcgcga ggctcaccgc cagcaccagc gccatgccga
     1261 ccagccagat cagcatcagg cgggcgaaca ggctgcgcgg cagcggcagg ctcatcgcgc
     1321 gccgctcccg tcggtgtccg cggtctcggc gacgacctcg gcggcgagca cgtagccctc
     1381 gttgcgcacg gtcttgatga tcgccggttc gcgcgcgttg tcgccgaggc gctggcgcag
     1441 ccggctcacc agcaggtcga tcgagcgatc gaacacgtcg gcctcgcgtc cctgggtgag
     1501 ttccatgagc tggtcgcgcg acagcacctt ctgcgggtgc gacaggaaga ccgcgagcat
     1561 gcggtactcg gcgccggaga gcgcgaccac cgtgccctcg gcgtcgacca ggtggcggtt
     1621 gatcgtgtcc aggcgccagt gcgcgaagcg cagcaggcgc gcgttggcgg gcggggcggc
     1681 gtcgaggttg ggcggcagcg cgcgggtgcg gcgcagcacc gcgcggatgc gggcgaagag
     1741 ctcgcgcggc acgaagggct tggcgaggta gtcgtcggca cccatctcga ggccgatgat
     1801 gcggtccatg tcctcgccgc gcgcggtgag catcaggatc ggggtgttgg cgtgcggccc
     1861 gccgctcgcg cgcaggttgc ggcacagggt gagtccatcc tcgccgggca tcatcaggtc
     1921 gagcacgatg aggtcgatgc ggtgggtctc gagcgctgcc ttcatctcgc gcccgtcggc
     1981 cgcggtggtg cagcgcagtc cctgcttctc gaggtagtcg gcaagcagct ggcggatgtc
     2041 acggtcgtcg tcgacgatga ggatgtggtc ggacgtattc atgcggtgtt ctccatggcc
     2101 tggatcataa ggcggggtgg gcccggcttt gtatcccagc gtatctgcgg gcgctgccga
     2161 gacatcaggc tgcaaacgcg tgccgcgcgg acaaatccgg cttacatcgc ggtgttcaaa
     2221 tggctccgtc gcccgaacgg acgggcaaca cggacaagca acccacagga gagccatcat
     2281 gaaaaactgg atcaagacct cgatcgccgc cgccatcatc gccaccggcg ccatcggcag
     2341 caccgcagcg ctggcctggg gcggccattg cgacggcccg cgcggcggca aggccggctg
     2401 gtcgcagatg gcgcccgagc agatgaaggc gaagatggcc gcgcgcgccg agctgcacat
     2461 ggcacgcctc gaactggcgc tggccctggc gcccgagcag aagcccgcct tcgagcgctt
     2521 caaggccgac atgaaggcgc gtgccgagcg catgggcagc atgatggccg agcgccgcgc
     2581 cgccgagccg ccgaagacgg cgatcgagcg catgcagcgc atggaggaga tgagcaagct
     2641 acgccaggcc gagatgggcg aggcgcgcaa gtcgatcgag accttctatg cgacgctgag
     2701 cgatgcgcag aagacggtgt tcgatgcgga gttcgagaa
//
LOCUS       DAOQNN010000425         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_27904_length_2725_cov_11.275655, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000425 DAOQNN010000000
VERSION     DAOQNN010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27904_length_2725_cov_11.275655"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1067
                     /locus_tag="PKV97_15615"
     CDS             105..1067
                     /locus_tag="PKV97_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB07362.1"
                     /translation="MPERFPGLARRLPWLVLALLAAFPLIAPAFGLEYYVGFVRRVLI
                     VAIAAASLNFILGYGGMAALGHAGFIGVGAYTVVALVEAGFSSAWLAWGSAAVLAGLF
                     AAAIGAVSLRTRGVYFIMITLAFAQMLHYLAVSLRTYGGDDGYGLYTPLALGAWLDGA
                     HAQVFYWVVLAIAAAVFALFSRVAHSRYGHALRGVRDNETRMVALGYPVYRLKLAGFV
                     AAGAVAGLAGGLLASHNAFVSPSIMHWTQSALLLVMVMLGGAGRRWGAPLGVALWLTL
                     EEVLKLYTDYWHWPLGLLLLLVVFHAPQGVAALLERRAGKEGAR"
     gene            1064..1813
                     /locus_tag="PKV97_15620"
     CDS             1064..1813
                     /locus_tag="PKV97_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB07363.1"
                     /translation="MSLFRAEGLVKRFGGLLATDHVSLAVEAGEIHALIGPNGAGKST
                     LVNLISGLLPADAGRIVLDGVELTALTPHARTRAGLARCFQISSVFRNDSVRDNLLLA
                     VQAQAGSSFRCLSRRDAEHALQERAEALAQRTGLAEVLERNAGTLAHGLQKRLDLALA
                     LAARPRLLLLDEPMAGMGPEDSQQMVALIRGLAGETAILLIEHDMDAVFQLADRISVL
                     VYGKVLTSGDAAHIKGHAEVQAVYLGTEVAG"
     gene            1810..2523
                     /locus_tag="PKV97_15625"
     CDS             1810..2523
                     /locus_tag="PKV97_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB07364.1"
                     /translation="MNAPLLCCRGLEAGYGTSRVLFGLDFDIRAGEVVALLGRNGMGK
                     STTIRTIVGGLRPQRGEIVFDGRPIHAERADAIARLGVAIVPEGRQCFPNLTVHEHLV
                     AFAARRNGVAEPWTPARLYALFPRLAERADHLGNQLSGGEQQMLAIARALSTNPRLLI
                     LDEATEGLAPVIREEIWRCLALLKAQGQTTLVVDKYVERLMALADRNLILERGRITWQ
                     GSSAELDARRELWGRYLGV"
BASE COUNT          324 a          970 c         1003 g          428 t
ORIGIN      
        1 gacctcggcc cggcgctcgc cggcatcgcc atgtacgtgc tgatggcggc ggtgctgagc
       61 ttgaaacccg cggggctgtt ccccgcgcgc ggctgaggga cgcgatgccc gaacgcttcc
      121 ccggcctcgc gcgccgcctg ccctggctgg tgctggccct gctcgcggcc ttcccgctga
      181 tcgcgccggc cttcgggctg gagtactacg tcggcttcgt gcgccgggtg ctgatcgtgg
      241 cgatcgcggc ggccagcctc aacttcatcc tcggctacgg cggcatggcg gcgctcggcc
      301 acgcgggctt catcggcgtc ggggcctaca ccgtggtggc gctggtcgag gccgggttct
      361 cgtcggcctg gctggcctgg ggcagcgcgg cggtgcttgc cgggctgttc gcagcggcga
      421 tcggcgcggt gtcgctgcgc acgcgcgggg tgtacttcat catgatcacc ctcgccttcg
      481 cgcagatgct gcactacctc gcggtgtcgc tgcgcacgta tggcggcgac gacggctacg
      541 gcctgtacac cccgctggcg ctgggtgcct ggctggacgg cgcgcatgcg caggtcttct
      601 actgggtggt gctggcgatc gccgcggcgg tgttcgcgct cttcagccgg gtcgcgcatt
      661 cgcgctatgg ccatgcgctg cgcggcgtcc gcgacaacga gacgcgcatg gtggcgctcg
      721 gctacccggt ctatcggctc aagctcgccg gcttcgtcgc cgccggcgcg gtggcggggt
      781 tggccggtgg cctgctcgcc agccacaacg ccttcgtcag cccgtcgatc atgcactgga
      841 cgcagtccgc gctgctgctg gtgatggtga tgctcggcgg cgccggccgg cgctggggcg
      901 cgccgctcgg ggtggcgctg tggctgacgc tggaggaggt gctgaagctg tacaccgact
      961 actggcactg gccgctcggg ctgttgctgc tgctggtggt gttccacgcg ccgcagggcg
     1021 tggcggcgct gctcgagcgc cgcgccggca aggagggcgc gcgatgagcc tgttcagggc
     1081 ggagggcctg gtcaagcgct tcggcggcct gctcgccacc gaccatgtct ccctcgccgt
     1141 cgaggcgggc gagatccatg cgctgatcgg ccccaacggc gcgggcaagt ccaccctggt
     1201 gaacctgatc tccggactgc tgccggcgga cgccgggcgc atcgtgctcg acggtgtcga
     1261 gctgaccgcg ctgacgccgc acgcgcgcac ccgtgccggc ctggcgcgct gcttccagat
     1321 cagcagcgtg ttccgcaacg acagcgtgcg cgacaacctg ctgctcgcgg tgcaggcgca
     1381 ggcggggtcg agcttccgct gcctgtcgcg gcgcgacgcc gagcacgcgc tgcaggagcg
     1441 cgccgaggcg ctggcgcagc gcacgggcct ggccgaggtt ctcgagcgca acgccggcac
     1501 cttggcccac ggcctgcaga agcggctcga cctcgcgctc gcgctcgcgg cgcggcccag
     1561 gctgctgctg ctcgacgagc ccatggccgg catgggaccg gaggattcgc agcagatggt
     1621 ggcgctgatc cgcggtctcg ccggcgagac cgcgatcctg ctcatcgagc acgacatgga
     1681 cgcggtgttc cagctcgccg accgcatctc ggtgctggtg tacggcaagg tgctgacctc
     1741 gggcgacgcc gcccacatca aggggcatgc cgaggtgcag gccgtgtatc tggggaccga
     1801 ggtggcggga tgaacgcgcc gctgttgtgc tgccgcggcc tggaggccgg ctatggcacc
     1861 agccgggtgc tgttcggcct cgacttcgac atccgcgccg gcgaggtggt ggcgctgctc
     1921 gggcgtaacg gcatgggcaa gagcaccacg atccgcacga tcgtcggcgg cctgcgcccg
     1981 cagcgcggcg agatcgtctt cgacggccgt ccgatccacg ccgagcgcgc cgacgccatc
     2041 gcccgcctcg gcgtggcgat cgtgcccgag ggccggcagt gcttccccaa tctcacggtg
     2101 cacgaacacc tcgtcgcctt cgccgcgcgc cgcaatggcg tcgccgagcc atggacgccg
     2161 gcgcggctct acgcgctctt cccgcgcctg gccgagcgcg ccgaccacct cggcaaccag
     2221 ctctccggcg gcgagcagca gatgctggcg atcgcgcgtg cgctgtcgac caacccgcgc
     2281 ctgctgatcc tcgacgaagc caccgaaggc ctcgccccgg tgatccgcga ggagatctgg
     2341 cgctgcctcg cgctcctcaa ggcgcagggg cagaccacgc tggtggtgga taaatacgtc
     2401 gagaggctga tggcgctcgc cgaccgcaac ctcatcctgg agcgcgggcg cattacctgg
     2461 cagggcagct cggccgagct ggacgcgcgg cgcgagctgt gggggcgcta cctgggggtg
     2521 tgagcggggc ggacggacgg ggcccccggc gagcagcgtg ccggctgcca ggggcgatgt
     2581 tcgccgctcc ctgcccctgt aggagcggcg gaagccgcaa atgaggcggc tccgatgtac
     2641 gccgtgctcg atccgccgcc gcgttcgcgc ctgccggcgc tcctgcatca acccccgcgc
     2701 gcgcccacca ttttccccga tccac
//
LOCUS       DAOQNN010000426         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_27954_length_2722_cov_11.099363, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000426 DAOQNN010000000
VERSION     DAOQNN010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27954_length_2722_cov_11.099363"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..592)
                     /locus_tag="PKV97_15630"
     CDS             complement(14..592)
                     /locus_tag="PKV97_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302438.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HNB07365.1"
                     /translation="MTETISRFKVPELADLAEDVRERILAVQEKAGFVPNVFLMLAHR
                     PAEFRAFFDYHDALMEKDVGLSKAEREMIVVATSAAGQCLYCVIAHGAILRIRAKNPR
                     LADQLATNHRKAEISPRQRAMLDFAIKLSTRGAEVDEADLAVLRAHGFDDEAIWDIGA
                     ITAFFSMSNRLVHLTGTPPNEPFYLMGRVPKA"
     gene            complement(629..1612)
                     /locus_tag="PKV97_15635"
     CDS             complement(629..1612)
                     /locus_tag="PKV97_15635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HNB07366.1"
                     /translation="MFKAVLIEKDDAGYRAAVKELDEAALPEGDVGVRVEYSSLNYKD
                     GLAITGRGAVVRKFPMVAGIDLVGTVESSAHPGVAVGDKVILNGWGTSEVHWGGLAQR
                     ARVRGEWLVPLPAAFTPRQAMAIGTAGYTAMLCVMALERHGVVPQRGEVLVTGASGGV
                     GSVAIALLSRLGFTVVASTGRPQEADYLRELGATTIIDRSELGLPGRALGKERWAGVV
                     DTVGSHTLANACATTLYRGTVAACGLAQGLDFPASVAPFILRGVTLVGIDCVMAPREE
                     RIEAWSRLARDLDVHKLERMTREIGLGEVVDTAAALLDGQERGRIVVDVDR"
     gene            1753..>2722
                     /locus_tag="PKV97_15640"
     CDS             1753..>2722
                     /locus_tag="PKV97_15640"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNB07367.1"
                     /translation="MKRVDDFRLKLGSQELVPIMVGGMGVDISTSDLALEAARLGGVG
                     HISDAMLPTVSDRRYNTKYVKNKLQQYKFNVANADKSVVQFDLGLIAEATATHVGRTM
                     ERKRGPGMVFINCMEKLTMNAPKETLRVRLRTAMDHGIDGITLAAGLHLGSLSLIEDH
                     PRFRDVQLGIIVSSLRALQLFLKKSARTGRLPDYVVVEGPLAGGHLGFGMDWAQYDLA
                     TIVAEIRDWMREQQLDIPLIPAGGIFTGTDAVNFLEAGAAAVQVATRFTVTKECGLPD
                     EVKQDYFKAEEDEIEVNQISPTGYPMRMLKSSPAIGSGIRPNCEAYG"
BASE COUNT          444 a          949 c          905 g          424 t
ORIGIN      
        1 cgcacctcgc acctcacgcc ttcggcaccc tccccatcag gtagaagggc tcgttgggcg
       61 gcgtaccggt cagatgcacc agccggttgc tcatgctgaa gaaggcggtg atcgcgccga
      121 tgtcccagat cgcctcgtcg tcgaagccat gcgcgcgcag gaccgcgagg tcggcctcgt
      181 cgacctcggc gccacgcgtg ctgagcttga tggcgaagtc gagcatcgcg cgctggcgcg
      241 ggctgatctc ggccttgcgg tgattggtgg cgagctggtc ggcgaggcgc gggttcttcg
      301 cgcggatgcg caggatggcg ccatgggcga tcacgcagta caggcactgg ccggcagccg
      361 aggtggcgac gacgatcatc tcgcgctcgg ccttgctgag gccgacgtcc ttctccatca
      421 gggcgtcgtg gtagtcgaag aaggcgcgga actcggccgg gcggtgggcg agcatcagga
      481 acacgttggg cacgaagccc gccttctcct gcacggcgag gatgcgctcg cgcacgtcct
      541 cggcaaggtc ggcgagttcg ggcaccttga agcggctgat ggtttcggtc attggaagtt
      601 tcctccgtcg atgtgtttat ggtcgggttc agcgatcgac atcgaccacg atgcgcccgc
      661 gctcctggcc gtcgagcagc gcggcggcgg tgtcgaccac ctcgccgagg ccgatctcgc
      721 gcgtcatgcg ctcgagcttg tgaacgtcga ggtcgcgggc gaggcggctc caggcctcga
      781 tgcgctcctc gcgcggcgcc atcacgcagt cgatgccgac gagagtgacg ccgcgcagga
      841 tgaagggtgc gaccgaggcc gggaaatcga gtccctgcgc caggccgcag gcggcgaccg
      901 tgccgcggta cagggtggtc gcgcaggcgt tggccagggt gtggctgccc accgtgtcga
      961 ccacccccgc ccagcgctcc ttgccgagcg cgcggccggg gaggccgagt tcgctgcggt
     1021 cgatgatggt ggtcgcgccc agttcgcgca ggtagtcggc ctcctgcgga cgcccggtgg
     1081 aggccaccac ggtgaagccc agcctggaga gcagcgcgat cgccacgctg cccacgccgc
     1141 cactcgcgcc ggtcaccagc acctcgccgc gctgcggaac gacgccgtgg cgctcgagcg
     1201 ccatcacgca cagcatcgcg gtgtagccgg cggtcccgat cgccatcgcc tggcgcgggg
     1261 tgaaggccgc gggcagcggc accagccact cgccgcgcac gcgcgcacgc tgcgcgagcc
     1321 cgccccagtg cacctcgctc gtcccccagc cgttgaggat caccttgtcg cccaccgcca
     1381 cgcctggatg cgcgctggac tcgaccgtgc cgacgaggtc gatgcccgcg accatcggga
     1441 atttgcgcac caccgcacca cgcccggtga tcgccaggcc atccttgtag ttcagcgaag
     1501 agtattccac ccgaacgccg acgtcgcctt cgggcagcgc ggcctcgtcg agctccttca
     1561 ccgccgcgcg gtagccggca tcatccttct cgatcagcac tgccttgaac atttttctct
     1621 ccctgggcgc acctcgcgcc ggtttcgcag tcgaacccgc atgttaaacg atgcccgcat
     1681 ggcgctcgaa cttgggccca aaggggctct cgggtgataa tgtcggactg ccgaatcaac
     1741 ggactccaag caatgaagcg tgtagatgac ttccgcctga aactgggctc ccaggaactc
     1801 gtgccgatca tggtgggcgg gatgggggtg gatatttcca cctccgatct ggcgctcgag
     1861 gccgcccggc tgggcggggt cggacacatc tccgatgcga tgctgccgac cgtgtccgac
     1921 cggcgctaca acaccaagta cgtcaagaac aagctgcagc aatacaagtt caacgtcgcc
     1981 aacgcggaca agtcggtggt gcagttcgac ctcggcctca tcgccgaagc gaccgcgacc
     2041 cacgtcggcc gcaccatgga gcgcaagcgc gggccgggca tggtgttcat caactgcatg
     2101 gaaaagctga cgatgaacgc gcccaaggaa accctgcgtg tgcgcctgcg caccgcgatg
     2161 gaccacggca tcgacggcat cacactggca gccggcctgc acctgggctc gctctccctc
     2221 atcgaggacc atccgcgctt ccgcgacgtg cagctgggca tcatcgtcag ctcgctgcgc
     2281 gcgctgcagc tcttcctcaa gaagagcgcg cgcaccgggc ggctccccga ctacgtggtg
     2341 gtcgagggcc cgctcgccgg cggccacctc ggcttcggca tggactgggc gcagtacgac
     2401 ctcgccacca tcgtcgccga gatccgcgac tggatgcgcg agcaacagct cgacatcccg
     2461 ctgatcccgg ccggcggcat cttcaccggc accgacgcgg tgaatttcct cgaggccggc
     2521 gccgcggccg tccaggtcgc gacccgcttc acggtcacca aggagtgcgg cctgcccgac
     2581 gaggtcaagc aggactactt caaggccgag gaggacgaga tcgaggtcaa ccagatctcg
     2641 cccaccggct acccgatgcg catgctgaag agcagcccgg cgatcggcag cggcatccgc
     2701 cccaactgcg aggcctacgg ct
//
LOCUS       DAOQNN010000427         2709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_28159_length_2709_cov_7.301809, whole genome shotgun sequence.
ACCESSION   DAOQNN010000427 DAOQNN010000000
VERSION     DAOQNN010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28159_length_2709_cov_7.301809"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /gene="cysS"
                     /locus_tag="PKV97_15645"
     CDS             <1..1088
                     /gene="cysS"
                     /locus_tag="PKV97_15645"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNB07368.1"
                     /translation="DALGVLRPDHEPRATDYVAQMQSLISRLEQKGLAYVAANRDVCY
                     AVRKFDGYGKLSGKSLDELRAGERVDVAQDKNDPLDFVLWKHAKVEEPGEVKWASPWG
                     EGRPGWHIECSAMSSDLLGDHFDIHGGGMDLQFPHHENEIAQSEGAHGHAFVNYWMHN
                     GFVRVDDEKMSKSLGNFFTIREVLQKYDPEVVRFFILRAHYRSALNYSDAHLEDARNA
                     LTRLYTALKNVPVAGAPVVDWSEPFAQRLRAAMDDDFNTAEAVAVLFELANEVNRSTA
                     PALAAQLKALGGVLGLLGRDPQAFLQAGAPDADGLDADAIEAKIAERAAAKKAKNFAE
                     ADRIRAELTEAGVILEDGPGGTTWRRA"
     assembly_gap    1375..1474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1669..>2709)
                     /gene="dnaJ"
                     /locus_tag="PKV97_15650"
     CDS             complement(1669..>2709)
                     /gene="dnaJ"
                     /locus_tag="PKV97_15650"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNB07369.1"
                     /translation="AMKFHPDRNPDNKEAEEKFKEAKEAYEMLSDPQKKAAYDRYGHA
                     GVDPSMGAGPGAQGFDGFADAFGDIFGDLFGGGGRGGRSNVYRGADLRYNLEITLEEA
                     ARGAEKTIRIPTVEECGTCHGSGAKPGTHPKPCPTCQGHGQVRVQQGFFSIQQTCPKC
                     HGSGKIIPDPCRDCGGAGRTKKQKTLEVKIPAGIDDGMRLRHAGHGEPGLNGGPPGDL
                     YVEIHIRKHAVFERDHDDLHCEMPISITTAALGGEIEIPTLEGMARLKIPAETQSGKV
                     FRLRGKGIKNVRSHVHGDLMCHVVVETPVNLTERQKELLREFEESASGNATRHNPKAQ
                     GWMDKVRDFFGG"
BASE COUNT          380 a          901 c          922 g          406 t
ORIGIN      
        1 ccgacgcgct cggcgtgctg cgccccgacc acgagccgcg cgccaccgac tacgtcgcgc
       61 agatgcagtc gctgatctcg cgcctggagc agaagggcct ggcctacgtc gccgccaacc
      121 gcgacgtgtg ctacgcggtg cgcaagttcg acggctacgg caagctgtcg ggcaagtcgc
      181 tcgacgagct gcgcgccggc gagcgtgtgg atgtggcgca ggacaagaac gacccgctcg
      241 acttcgtgct gtggaagcac gccaaggtcg aggagccggg cgaggtgaag tgggcctcgc
      301 cctggggcga gggccggccg ggctggcaca tcgaatgctc ggcgatgagc tcggacctgc
      361 tcggcgacca cttcgacatc cacggcggcg gcatggacct gcagtttccg caccacgaga
      421 acgagatcgc ccagtccgaa ggcgcgcacg gccatgcctt cgtcaattac tggatgcaca
      481 acggcttcgt gcgcgtcgac gacgagaaga tgtcgaagtc gctcggcaac ttcttcacca
      541 tccgcgaggt gctgcagaag tacgaccccg aggtggtgcg cttcttcatc ctgcgcgcgc
      601 actaccgcag cgcgctcaac tactccgacg cccacctcga ggacgcccgc aacgcgctca
      661 cccgcctgta caccgcgctc aagaacgtgc cggtggccgg ggcgccggtg gtcgactgga
      721 gcgagccctt cgcgcagcgc ttgcgcgcgg cgatggacga cgacttcaac accgccgagg
      781 cggtggccgt gctgttcgag ctcgccaacg aggtcaaccg cagcaccgcg ccggcgctcg
      841 ccgcgcaact gaaggcgctc ggcggcgtgc tcggcctgtt gggacgcgac ccgcaggcct
      901 tcctgcaggc gggtgcgccc gacgcggacg ggctggacgc cgacgcgatc gaggcgaaga
      961 tcgccgaacg cgcggccgcc aagaaggcga agaacttcgc cgaggccgac cgcatccgcg
     1021 cagagctgac cgaggccggc gtgatcctcg aggacggccc cggcggtacc acctggcgcc
     1081 gcgcctgagc ctgcagcggc cagcggggcg actgcgctgg tcggcatcac cgctgtgctg
     1141 tggcgcaggt caccgcggta tcgccaggcg ctgccgtggc tggccgagtt gaatccaaac
     1201 gatccgaacc cgtacaagga accccgcaca tgatcatcac cggctttcag agcctgctcg
     1261 acgccgcgcg ccagcaaccc gaaccgcagc gcctgctgct cgtcttcact cgcgccgaac
     1321 tgcccgagaa ggccaccgcc gagcagcgcg ccaagttcga gcagggcgag ggcgnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcgtt ggccggcgtg acggtttccg
     1501 tgggagcgac gcaagtcgcg atcgacgtta cgagccgcga cgcgcggcgg cgggcgtgac
     1561 ggtttccgtg ggagcgacgc aagtcgcgat cgacgttacg agccgcgacg cgcggcggcg
     1621 ggcgcgaggc ggctgaaacc gggcccgccc cgcgcccgcg aacaggcgtc agccgccgaa
     1681 gaagtcccgc accttgtcca tccagccctg cgccttgggg ttgtggcgcg tggcgttgcc
     1741 gctggcgctc tcctcgaact cgcgcagcaa ctccttctga cgctcggtca ggttcaccgg
     1801 ggtctcgacc accacgtggc acatcaggtc gccgtgcacg tggctgcgca cgttcttgat
     1861 gcccttgccg cgcagccgga agaccttgcc gctctgcgtc tccgcgggga tcttcagccg
     1921 cgccatgcct tccagcgtcg ggatctcgat ctcgccgccg agcgccgcgg tggtgatgct
     1981 gatcggcatc tcgcagtgca ggtcgtcgtg gtcgcgctcg aacaccgcgt gcttgcggat
     2041 gtggatctcg acgtagaggt cgcccggcgg gccgccgttg aggccgggct cgccgtggcc
     2101 ggcgtggcgc aggcgcatgc cgtcgtcgat gccggcgggg atcttcacct cgagcgtctt
     2161 ctgcttcttg gtgcgtccgg cgccgccgca gtcgcggcag gggtcgggga tgatcttgcc
     2221 gctgccgtgg cacttcgggc aggtctgctg gatcgagaag aagccttgct gcacgcgcac
     2281 ctggccgtgg ccctggcagg tggggcaggg cttgggatgg gtgccgggct tggcgccgct
     2341 gccgtggcag gtgccgcact cctcgacggt ggggatgcgg atcgtcttct cggcgccgcg
     2401 cgcggcctct tccagggtga tctcgaggtt gtagcgcagg tcggcgccgc gatagacgtt
     2461 cgagcgcccg ccgcgtccgc cgcccccgaa gaggtcgccg aagatgtcgc cgaaggcgtc
     2521 ggcgaagccg tcgaagccct gcgcgccggg gcccgcgccc atcgacggat cgacgccggc
     2581 gtggccgtag cggtcgtagg cagccttctt ctgcgggtcg gagagcatct cgtaggcctc
     2641 cttggcctcc ttgaacttct cctcggcttc cttgttgtcc ggattgcggt ccggatgaaa
     2701 cttcatggc
//
LOCUS       DAOQNN010000428         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_28173_length_2708_cov_7.742179, whole genome shotgun sequence.
ACCESSION   DAOQNN010000428 DAOQNN010000000
VERSION     DAOQNN010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28173_length_2708_cov_7.742179"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PKV97_15655"
     CDS             complement(<1..808)
                     /locus_tag="PKV97_15655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB07370.1"
                     /translation="MRSTTEPTVSVVIPAFNAAWCIRRAVDSVLAQDFTDFELIVVDD
                     GSTDDTAEVLRGYGDALRLVSQPNGGMSRARNAGIRLARGRYIAFLDADDRWLPAKLG
                     RQVALLDQRPELAFCAATASLEDPDGKPVGTWACQGDGTASVAEVFAAHALIAGGASS
                     VLARREPVQALGGFDETLFGAEDTDLWIRLAACGGFACIPEALVVVLKRPGSVSRNRA
                     RMRAGALAMTRKNRPLLPADKRGAYWRALYAGTLCDYAKWACREGDRGAAL"
     gene            complement(792..1754)
                     /locus_tag="PKV97_15660"
     CDS             complement(792..1754)
                     /locus_tag="PKV97_15660"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNB07371.1"
                     /translation="MSSFRLSHLHKLLDPEIRAKNLQWLRWRARELYWSTTHPAMPDP
                     VFVIGCSRSGTTVTFETLAASGQYLHFDYELPQLWDGLYGPLNNGWASEAAGPEHADH
                     AHRERALAYFYARLGAGPVLDKTCINTLRVAYLHKLFPRAKFVFIHRDGRDNISSMID
                     GWRLGRTDGGFGLEKFFGPSPVPVEINGGEFHEWHFFLPPGWRDYNRASLEEVCAFQW
                     ISANRLALDAARDVPAAQWIQVRYEDIFERPVEMFRTVFERLGLPFDARMEAHCAGLS
                     GRPTSIVKGTPKKQKWKESNPEAIARILPAIAPLMRELGYALDD"
     gene            complement(1795..>2708)
                     /locus_tag="PKV97_15665"
     CDS             complement(1795..>2708)
                     /locus_tag="PKV97_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB07372.1"
                     /translation="TPFITVALCTHNHADRLARTLETLADLHPPERPWELLVIDNGCT
                     DATPALLARTAWHPAGVAVRVVREERLGLSNARNRALREARGEYLLFMDDDETPAPEW
                     LRAYEQAMEAFEPDALGGRIEVFFEDGVRPRWLQDELLGFLGKLDHGEARWLDAPGTP
                     IFGGNFAFRRGVFDTIGDFDARLGRMGKANIGGEDTEIYRRLLAHGCRVRWVPQAIIH
                     HRIQTPKLRRGYFLDLHFRQGRTEGSAKRGSGRRIPPPYLIAQWLRALGRAAGLRLRA
                     GADHSLRLEMNAAYFCGFLLGWMRDRG"
BASE COUNT          400 a          905 c         1003 g          400 t
ORIGIN      
        1 cgagcgcagc gccgcgatcg ccctcgcggc aggcccactt ggcgtagtcg cacaacgtgc
       61 cggcatagag tgcgcgccag taggcgccgc gcttgtcggc gggcaggaga gggcggttct
      121 tgcgcgtcat cgccagtgcg ccggcgcgca tccgcgcgcg gttccggctc acgctgccgg
      181 ggcgcttgag taccaccacc agcgcctcgg ggatgcatgc gaagccgccg catgcggcca
      241 gacggatcca caggtccgta tcttccgcgc cgaacagggt ctcgtcgaaa ccgccgagcg
      301 cctgcaccgg ctcgcgtcgc gcgagcaccg acgaggcgcc ccccgcgatc agcgcatggg
      361 cggcgaatac ctcggccacc gaggcggtgc cgtcgccctg gcaggcccag gtgccgaccg
      421 gcttgccgtc cgggtcctcg aggctcgccg tcgcggcgca gaaggccagc tccgggcgct
      481 gatcgagcag tgcgacctgg cgcccgagct tggcggggag ccagcggtcg tcggcgtcga
      541 ggaaggcgat gtagcgcccg cgtgcgaggc ggatgccggc gttgcgcgcc cggctcatgc
      601 cgccgttggg ctgggagacg aggcgcagcg cgtcgccgta gccgcgcagg acctccgcgg
      661 tgtcgtcggt actgccgtcg tccacgacga tcagctcgaa atcggtgaag tcctgcgcca
      721 gcacgctgtc gaccgcgcgc cggatgcacc aggcggcgtt gaaggccggg atgacgacgc
      781 tgaccgtcgg ctcagtcgtc gagcgcatag cccagctcgc gcatcagcgg agcgatcgcc
      841 ggcaggatgc gcgcgatcgc ctcggggttg ctctccttcc atttctgctt cttcggcgtg
      901 cccttgacga tgctggtcgg gcgccccgag agtcctgcgc agtgggcctc catgcgcgcg
      961 tcgaagggca ggccgaggcg ttcgaacacg gtgcggaaca tctccaccgg gcgctcgaag
     1021 atgtcttcgt agcgcacctg gatccactgc gccgccggca cgtcgcgcgc cgcgtcgagg
     1081 gcgagccggt tggcgctgat ccactggaac gcgcacacct cttccaggct ggcccggttg
     1141 tagtcgcgcc accccggcgg caggaagaag tgccattcgt ggaactcgcc gccgttgatc
     1201 tccaccggca ccggcgacgg gccgaagaat ttctccaggc cgaagccgcc atcggtacgg
     1261 cccagccgcc agccgtcgat catcgagctg atgttgtcgc gtccatcgcg gtggatgaag
     1321 acgaacttcg cgcgcgggaa cagcttgtgc aggtaggcca cgcgcagcgt gttgatgcag
     1381 gtcttgtcga gcaccgggcc ggcgccgagg cgcgcataga agtaggcgag cgcgcgttcg
     1441 cggtgcgcgt ggtcggcatg ttccgggcct gcggcttccg atgcccagcc gttgttcagc
     1501 ggcccgtaca gcccgtccca gagctgcggc agctcgtagt cgaaatgcag gtactgcccg
     1561 gacgcggcga gcgtctcgaa ggtgacggtg gtgcccgagc gcgagcagcc gatcacgaag
     1621 accgggtcgg gcatggccgg atgggtggtc gaccaataga gctcccgggc ccgccagcgc
     1681 agccactgga ggttcttggc gcggatctcg gggtcgagca gcttgtgcag gtgcgacagc
     1741 cggaacgatg acatcggggc aggtccaatc agagagacag ggggaggcgg cggctcagcc
     1801 gcggtcgcgc atccagccga gcaggaaacc gcagaagtag gccgcgttca tctccagccg
     1861 cagcgaatga tcggcgcccg cgcgcagcct gagtccggcg gcgcggccga gggcgcgcag
     1921 ccattgcgcg atcaggtagg ggggcgggat gcgtcggccg ctgccgcgct tcgccgaccc
     1981 ttcggtgcgt ccctggcgga agtgcaggtc gaggaaatag ccgcggcgca gcttcggggt
     2041 ctggatgcgg tggtggatga tcgcctgcgg aacccagcgc acccggcagc cgtgcgccag
     2101 caggcgacgg tagatctcgg tgtcctcgcc gccgatgttg gccttgccca tgcgtccgag
     2161 gcgggcgtcg aaatcgccga tggtgtcgaa gacgccgcgg cggaaggcga agttgccgcc
     2221 gaagatcggc gtccccggcg catccagcca gcgtgcctcg ccgtggtcga gcttgccgag
     2281 gaagccgagc agctcgtcct gcagccagcg cggccggacg ccgtcctcga agaagacctc
     2341 gatgcggccg cccagcgcgt cgggctcgaa cgcctccatg gcctgttcat aggcgcgcag
     2401 ccactcgggg gcgggcgtct cgtcgtcgtc catgaacagc aggtactcgc cgcgtgcctc
     2461 gcgcagtgcg cggttgcgcg cgttcgacaa gccgaggcgt tcttcccgca ccacccgcac
     2521 cgcaaccccc gcggggtgcc acgcggtgcg cgcgagcagt gcgggcgtgg cgtcggtgca
     2581 gccgttgtcg atcacgagca gctcccaggg gcgttcggga ggatgcagat cggcgagcgt
     2641 ttcgagcgtg cgtgcgaggc ggtcggcgtg gttgtgggtg cacagtgcga ccgtgatgaa
     2701 gggcgtgg
//
LOCUS       DAOQNN010000429         2707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_28193_length_2707_cov_10.581825, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000429 DAOQNN010000000
VERSION     DAOQNN010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28193_length_2707_cov_10.581825"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKV97_15670"
     CDS             complement(<1..266)
                     /locus_tag="PKV97_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HNB07373.1"
                     /translation="MEPTMLGPTRRPWLLAAVLCVLTALAALPARAALDPTLVRGLGG
                     DFNARVAAIDALGVSGGDEAAALLDALESGRLGTLDGRVVIVAP"
     gene            complement(439..1692)
                     /gene="urtA"
                     /locus_tag="PKV97_15675"
     CDS             complement(439..1692)
                     /gene="urtA"
                     /locus_tag="PKV97_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HNB07374.1"
                     /translation="MNRRNFVKALTLSASIAAIGLPAGAHAADTIKVGILHSLSGTMA
                     ISETALKNVALMTIEEINAGGGVLGKKLEPVVVDPASNWPLFAERARQLLAQDKVAAV
                     FGCWTSVSRKSVLPVFKELNGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMS
                     EEGGGAKRFVLLGTDYVYPRTTNKILRAFLKSKGVSDADILEDYTPFGHADYQTIIAR
                     IKQFASEGKKTAVVSTINGDSNVPFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKP
                     LLGHLAAWNYFMSVDNPQNKAFIDKYRAWAKKNGVPNADTVVTNDPMEATYVGLHMWK
                     QAVEKAASTDVDKVIAAMGGQSFKAPSGFTLTMDATNHHLHKPVLIGEVRADGQFDVV
                     WQTKGPIRAQPWSPFIEGNEGKQGL"
BASE COUNT          408 a          906 c          954 g          439 t
ORIGIN      
        1 tcgggcgcga cgatcaccac ccggccgtcg agcgtgccga gtcgcccgct ttcgagcgcg
       61 tccagcagcg ccgcggcttc gtcgccgccg gaaacgccga gcgcgtcgat cgcggcgacg
      121 cgggcgttga agtcaccgcc cagtccgcgc accagcgtgg ggtcgagcgc cgcccgcgcc
      181 ggcagcgcgg cgagcgccgt cagcacgcac agcacggcgg cgagaagcca tggtcgtctt
      241 gtcggtccga gcatcgtcgg ctccatcttc agttgcgggg aaacagccgg ggcggcgggc
      301 ggttcggcag gcgtggggcc aagggggcgt gcgggatctc gccgcaagag gctcgcggcg
      361 tgcgccgctc ctgcaggaga accgccgcgc tccggaccgg cggcgaaaat cggggtcgga
      421 ccccgatctt ctggacgatc acagcccctg cttgccctcg ttgccctcga tgaacgggct
      481 ccacggctgg gcgcggatcg gccccttggt ctgccatacc acgtcgaact ggccgtccgc
      541 gcgcacctcg ccgatcagca ccggcttgtg caggtggtgg ttggtcgcgt ccatggtcag
      601 cgtgaagccc gacggcgcct tgaagctctg cccgcccatc gccgcgatga ccttgtcgac
      661 gtcggtgctg gcggccttct cgaccgcctg cttccacatg tgcaggccga cgtaggtggc
      721 ctccatcggg tcgttggtga ccacggtgtc ggcgttgggc acgccgttct tcttcgccca
      781 cgcgcggtac ttgtcgatga aggccttgtt ctgcgggttg tcgaccgaca tgaagtagtt
      841 ccacgccgcg aggtggccga gcaggggctt ggtgtcgacg ccgcgcagct cctcctcgcc
      901 gacggagaag gccaccaccg gcacgtccgt cgccttcagc ccggcattgc ccagttcctt
      961 gtagaagggc acgttggagt cgccgttgat ggtcgacacc acggccgtct tcttgccctc
     1021 ggaggcgaac tgcttgatgc gcgcgatgat ggtctggtag tcggcgtggc cgaagggcgt
     1081 gtagtcctcc aggatgtccg catcgctcac ccccttgctc ttcaggaagg cgcgcaggat
     1141 cttgttggtc gtgcgcggat acacgtagtc ggtgccgagc agcacgaagc gcttcgcgcc
     1201 gccgccttcc tcgctcatca ggtactcgac cgcgggaatc gcctgctggt tgggcgcggc
     1261 gccggtgtag aagacgttct tctcgagttc ctcgccctcg tactgcaccg ggtagaagag
     1321 caggccgttc aactccttga acaccggcag caccgacttg cgcgacaccg aggtccagca
     1381 gccgaacacc gccgcgacct tgtcctgcgc cagcagctgg cgcgcgcgct cggcgaacag
     1441 cggccagttc gaggccgggt cgaccaccac cggctcgagc ttcttgccga gcacgccgcc
     1501 gccggcgttg atctcctcga tggtcatcag cgccacgttc ttgagcgcgg tctcggagat
     1561 cgccatcgtg cccgacagcg aatgcaggat gccgaccttg atggtgtcgg cggcgtgcgc
     1621 gccggcgggc aggccgatcg cggcgatgga agccgaaagc gtgagggctt tgacgaagtt
     1681 gcgacggttc atggacggac ctcttgcagt gggtggaagt cgagcgcagc gcaggctgcg
     1741 tcgatgaatc ctctgcaagg ggcgtgccag gttgtcgccg aattaacgga tggagtgtgg
     1801 gacgcggccc aaatccgcgc cgttgatgcg ctcgtggcac gccgacggat cttgtgcccg
     1861 ctctcctggc gtccctgcag cgcgccttcg tcgctgatgc cgcgccccgc gccgatgcag
     1921 tacccggaag acgtgcacca tcgcagtgca gcaaactccc ggccacgcgt acgcggcggg
     1981 tcttgtcgag gtacccggtg ccctggccga tcgcgactcg cgtcgctcct acgaaaaccg
     2041 ccccgatgcg atccgggacc tgtaggagcg gcgcaagccg cgatcgggtg gtccggctgc
     2101 gacgggcgtt ggcgggggaa gcaggctgca atcgcgactt gcgtcgctcc cacgaaaacc
     2161 accccgatgc gatccgggac ctgtaggagc ggcgcaagcc gcgatcgggt ggtccggctg
     2221 cgacgggcgt tggcggggga agcaggctgc aatcgcgact tgcgtcgctc ccacgcaaac
     2281 cacctcgacg cgatccggga cctgtaggag cggagcaagc cgcgatcggg tcgtccggct
     2341 gcgacgggcg ttggcggggg aaggaggctg caatcgcgac tcgcgtcgct cccacgcaaa
     2401 ccaccccggc gcgatccgga acctgtagga gcggcgcaag ccgcgatcgg gtcgtccggc
     2461 tgcgacgggc gttggcgggg gaaggaggct gcaatcgcga cttgcgtcgc tcccacgcaa
     2521 accaccccga tgcgatccgg gacctgtagg agcggcgcaa gccgcgatcg ggtggtccgg
     2581 ctgcgacggg cgttggcggg ggaaggaggc tgcaatcgcg actcgcgtcg ctcccacgaa
     2641 aaccaccccg acgcgatccg ggacctgtag gagcggcgca agccgcgatc gggtggtccg
     2701 gctgcga
//
LOCUS       DAOQNN010000430         2685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_28589_length_2685_cov_7.744487, whole genome shotgun sequence.
ACCESSION   DAOQNN010000430 DAOQNN010000000
VERSION     DAOQNN010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28589_length_2685_cov_7.744487"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKV97_15680"
     CDS             <1..403
                     /locus_tag="PKV97_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07375.1"
                     /translation="LLAPARLALVTDALRAVAAARAGLPRRVPLALKFRLGGADWANE
                     APPAVALAAAAAGFDLLTAVLPDDDPKRLSRLHVLITAVSDGPAVVAVGGIRKASDVA
                     EVRASGAAGVQVHRAFSESGATCLAQLLGSA"
     gene            552..797
                     /locus_tag="PKV97_15685"
     CDS             552..797
                     /locus_tag="PKV97_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stability protein"
                     /protein_id="HNB07376.1"
                     /translation="MPTTLTLKNIPDEIYQRLKASAVTHRRSLNSEAIVCLETVLLPG
                     RIAPSERLARARALRAALPASKFRAHDIDALKREDRL"
     gene            800..1189
                     /locus_tag="PKV97_15690"
     CDS             800..1189
                     /locus_tag="PKV97_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNB07377.1"
                     /translation="MVDTNVLAYLYLPTEFTTHAEALLEHDPEWVAPVLWRSEFRSIL
                     AGYLRRKTLAFDDARALQAEAESLLAGNEHEVDSQRVLEFVRDTDCSAYDCEFAALAL
                     RLDVKLVTMEARLLRAFPKFAVPLTAA"
     gene            complement(1303..1818)
                     /locus_tag="PKV97_15695"
     CDS             complement(1303..1818)
                     /locus_tag="PKV97_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB07378.1"
                     /translation="MSVVASPVSPSSLAADDRKRHRPPGPLLGFAPADAERILAHLVA
                     LDVDDRFLRFGYGIGDEGIARYVARIDFARDHVHGLTDAGGRVVALAHMAVRDGEVDF
                     GLSVAPAWRRRGWGRTLFAHVVELAPLLRAQRVLCHSISPAVLHMAGASGFRRVGGNP
                     AAPLVLDIPVG"
     regulatory      complement(1975..2182)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2289..>2685)
                     /locus_tag="PKV97_15700"
     CDS             complement(2289..>2685)
                     /locus_tag="PKV97_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNB07379.1"
                     /translation="AHPFDPAERPLDDPHGLGAHKTTLRARYDEGLRAALAAGAFDTL
                     FFDSAGRLTEGARSNVFLLLDGEWRTPPAGRGVLPGTMRAALLEDPAWAAREAELRVE
                     DLLRAQRIVLTNALRGAVEARLERAVGGR"
BASE COUNT          418 a          900 c          970 g          397 t
ORIGIN      
        1 gctgctggcg cccgcaaggc tggcgctcgt cacggacgcg ctgcgcgcgg tggcggcagc
       61 gcgcgccgga ctgccgcgcc gggttccgct cgcgctcaag ttcaggctcg gcggggcgga
      121 ctgggcgaat gaggcgccgc cggcggtcgc cctcgccgcc gcggcagcag gcttcgacct
      181 gctgacggcc gtgctgccgg acgacgaccc gaagcgcctc tcgcgcctgc acgttctgat
      241 caccgcagtg tccgacggtc cggccgtggt ggcagtgggc ggcatccgca aggcaagcga
      301 cgtcgccgaa gtccgcgcga gcggcgctgc gggcgtgcag gttcaccgcg cattctccga
      361 atcgggggcg acctgtctgg cgcagttgct gggcagcgcc tagcaagctg gcggactacc
      421 tctgccgccc tcgttgcagg attggctgcc cgaatcgcac ctggcgcgcg atgtggtcga
      481 tatggtggaa atatcggctt gacgcactgg cctcaagaac cattatggtt ccactttgga
      541 aggagcctgc catgcccact acgctgaccc tgaagaatat tccagatgag atataccaac
      601 gtctgaaggc gtcggcggta acgcacaggc gcagcctgaa cagcgaagcg atcgtgtgcc
      661 tggagaccgt gttgctgccg ggcaggatcg ccccgagtga gcgactggcc cgtgcccgcg
      721 cattgcgagc tgcactgcca gcgtcgaagt tcagggcgca cgacatcgac gccttgaagc
      781 gcgaggaccg cctgtgatcg tggtggatac caatgtcctg gcgtacctgt acctgccgac
      841 cgagttcact acacatgccg aggcgctgct ggagcatgac ccggagtggg tggcgccggt
      901 gctgtggcgc agcgagttcc gcagcattct cgcgggttac ctgcgacgaa agaccctggc
      961 tttcgatgat gcgcgcgcct tgcaggccga ggcggagagc ctgcttgcgg ggaacgagca
     1021 tgaagtcgac tctcagcggg tactggagtt cgtgcgcgac accgattgtt ctgcgtatga
     1081 ctgcgagttt gccgcgctgg ccctgcgcct ggacgtgaaa ctggtgacga tggaagccag
     1141 gctgctcagg gcattcccga agttcgcggt gccgctcact gcggcgtgag ccctggattc
     1201 agcggtttgg cgcaccttct cgctgaagct ccgccgaaat ggcttccctc gcgtcgccgg
     1261 agccggctga aaaaccggtc gagtgctccg gtagccgagg cgtcagccga ctggaatgtc
     1321 cagcaccagg ggcgcggccg gattgccgcc cacgcggcga aaaccgctgg cgccggccat
     1381 gtgcagcacc gcggggctga tgctgtggca cagcacgcgc tgcgcgcgca gcaggggtgc
     1441 gagctcgacg acgtgggcga acagggtccg gccccagccg cggcggcgcc aggcgggtgc
     1501 cacgctgaga ccgaaatcga cctcgccatc gcggacggcc atgtgcgcga gcgccacgac
     1561 ccttccgccc gcgtcggtca ggccgtggac gtggtcgcgc gcgaagtcga tgcgggcgac
     1621 gtagcgggcg atcccctcgt caccgatgcc gtagccgaag cgaaggaagc gatcgtcgac
     1681 atcgagcgcc acgaggtggg cgaggatgcg ctcggcatcc gccggcgcga acccgagcag
     1741 cgggccggga gggcggtgcc gcttgcgatc atccgcagcg agggaactgg gcgagactgg
     1801 actggcgacg acggacatgg cggagacccc gggcaggcgg gcgggatctc cggcggacgc
     1861 gtcccagcct ccccggcgcc gcgaagggcg cagggaactg ccggatcatc tgcgagacga
     1921 gcaagtgaaa tggtatggac catgcctctt gtcagggaca ccccgcccta aacgaatgaa
     1981 ggtgatgtcc atggcaggtc tcctggcttg cgggtcctcg cctcgtgcca gccttcccgg
     2041 agagactcca gtggccagat gggcacgcga ctcgtcgcat acagttgcgg gggcagctcc
     2101 ggcttgcacg cgcgggcgtg ttaccggatt cccttttcat gccggccggt gagggcaggc
     2161 aaccatgaaa cgcggccgag gttagccgcg cgcctccagc ctgtcaaacc actcccggga
     2221 tccgcccgcg gatgccggcc acacgctggc gagccaccct gggtcggacc agagcactcg
     2281 ccgccggctc agcgaccgcc gacggcgcgt tccagcctcg cctccaccgc cccgcgcagc
     2341 gcgttcgtca gcacgatgcg ctgcgcgcgc agcaggtcct ccacgcgcag ctcggcctcg
     2401 cgcgcggccc aggccggatc ctccagcagc gccgcgcgca tcgtgccggg cagcacgccg
     2461 cggccggcgg ggggggtgcg ccactcgccg tccaggagca ggaagacgtt gctgcgcgcg
     2521 ccctcggtga ggcggcccgc cgagtcgaag aacagcgtgt cgaaggcgcc cgctgccagc
     2581 gcggcgcgca ggccctcgtc gtagcgcgcg cgcagcgtgg tcttgtgcgc gccgaggccg
     2641 tgcgggtcgt cgagcgggcg ctcggcgggg tcgaacgggt gcgcg
//
LOCUS       DAOQNN010000431         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_29098_length_2658_cov_13.313100, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000431 DAOQNN010000000
VERSION     DAOQNN010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29098_length_2658_cov_13.313100"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKV97_15705"
     CDS             complement(<1..194)
                     /locus_tag="PKV97_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB07380.1"
                     /translation="MLAKIIDWSGRNRFLVLLATLFILAAGVFAVLRTPIDALPDLSD
                     VQVIVYTEYPGQAPQVVEDQV"
     gene            complement(208..1827)
                     /locus_tag="PKV97_15710"
     CDS             complement(208..1827)
                     /locus_tag="PKV97_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB07381.1"
                     /translation="MKTGAGIAVGLAVTLAAGGGYWFGQRPMASHGAPAASVAAVPAE
                     GAKKERKLLYYRNPMGLPDTSPTPKKDPMGMDYIPVYEGEADETPAAARWIKISTEKV
                     QKLGVRTEVAELRSLDRTVRAAGRIEIDERRSFAIAPKFEGYVERLHVNVTGQPVAQG
                     QPLFEVYSPELVSAQREYAIAAQGVEALKGAGSEAQSGMKQLAESSLLRLRNWDISEE
                     QIRALAKSGATKRTLTFRSPVAGIVTEKKALQGMRFMPGDTLYQVADLSSVWVIADVF
                     EQDIGQVRSGALAKVRINAYPDKIFEGKVSYVYPTLNAETRTVPVRVELANPGQLLKP
                     AMFAQVEVPVGGKGQVVTVPNSAVIDSGTRQIVLVAHGEGRFEPRDVKLGGRSENHIE
                     VMDGVKAGEKVVVAANFLIDAESNLKAAVGGFGHSGHGGAPKSGKDGEKPAAAPQAAG
                     HRAEGTVDSVDAKDGTVSLSHGPVASLKWPAMTMEFKVANPSLLQALKPGVKVDVEFV
                     ERQPGEWVITSAAPAGKAATPAPTANPHAGH"
     gene            complement(1824..>2658)
                     /locus_tag="PKV97_15715"
     CDS             complement(1824..>2658)
                     /locus_tag="PKV97_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB07382.1"
                     /translation="LQYVYRNEQLTREILDLMVRLEKIAQVRYAGGLAAQQDVIRAQV
                     EQTSMKNELVALQNERRQLQARLNALLARPTSSPLAAPERLRTLPAPAQLDPTTLEER
                     ARTKNPQIFADEARIRSAEKSRELTYKNRYPDFTLGVSPIQYQGAVREWELMVELNLP
                     LQQGSRRAQERESEAMLSAAKARRDATANQVLGDLAENLSGFDAARRTENLASTSLLP
                     QSELTFKAALAGYETGKVDFATLLDAQRQIRQARQSQIKAQAEAQIRLADIERILGED
                     L"
BASE COUNT          420 a          870 c          847 g          521 t
ORIGIN      
        1 acctggtcct cgaccacctg cggcgcctgg ccggggtatt cggtgtagac gatgacctgc
       61 acgtcggaga ggtcgggcag cgcgtcgatg ggcgtgcgca gcaccgcaaa caccccggcc
      121 gcgagaatga acagtgtggc gagcagaacc aggaagcggt tccggcccga ccagtcgatg
      181 attttggcca gcatgatgga tcctgtgtca gtggccggcg tgaggattgg ccgtcggcgc
      241 cggcgtagcc gctttgccgg caggggctgc cgaggtgatg acccattcgc cgggctggcg
      301 ctcgacgaat tcaacatcca ccttcacgcc cggcttcaag gcctggagca gcgacggatt
      361 ggccaccttg aactccatgg tcatcgccgg ccacttgagg ctggcgaccg ggccgtgact
      421 caagctgacc gtaccgtcct tggcatcgac actgtccacc gtgccttcag cccggtgccc
      481 ggccgcttgg ggcgccgccg caggcttctc gccgtccttt ccggacttcg gtgcaccacc
      541 atggcccgaa tgaccaaagc caccgaccgc cgctttcagg ttgctttccg catcgatcag
      601 gaagttggcg gcgacaacca ccttttcgcc ggccttgacg ccatccatca cttcgatatg
      661 gttctcgcta cgcccaccca gcttcacgtc ccgcggctcg aagcgccctt cgccgtgggc
      721 gacgaggacg atctggcgag tgccgctatc aatcaccgcc gaattcggca cggtcacgac
      781 ctggcccttg ccccccaccg gcacctctac ctgcgcaaac atcgcgggtt tcaggagttg
      841 ccccgggttg gcgagctcga cccggacggg cacggtccgc gtttccgcat tcaaggtcgg
      901 atagacatag ctcaccttgc cctcgaagat cttgtccgga taggcgttga tgcgcacctt
      961 ggccagcgcg ccgctacgca cctggccaat gtcctgctcg aagacgtcgg cgatgaccca
     1021 taccgacgac agatcggcca cctggtacaa ggtgtcgccg ggcatgaagc gcatgccttg
     1081 cagcgccttc ttctcggtga cgataccggc caccggcgag cggaaggtca gcgtgcgctt
     1141 ggtcgcgccc gacttggcga gggcgcggat ctgctcctcc gaaatgtccc agttgcgcag
     1201 gcgcagcagg ctggattcgg cgagctgctt catgccgctc tgcgcttcgc tgccggcccc
     1261 cttgagcgct tcgacaccct gggcggcgat ggcgtattcc cgttgggcgg acaccagttc
     1321 cgggctgtag acctcgaaca gcggctggcc ttgggcgacc ggctggccag tcacgttcac
     1381 gtgcagacgc tcgacgtagc cctcgaactt cggggcgatg gcgaaactgc gtcgttcatc
     1441 gatctcaatg cggccagcgg cccgtaccgt gcggtccaga gaacgcagtt cggcgacctc
     1501 ggtacgcaca ccaagctttt gcactttctc ggtactgatc ttgatccatc tggctgctgc
     1561 gggcgtctcg tccgcctcgc cttcatagac tgggatgtag tccattccca tcgggtcctt
     1621 cttgggcgtc ggtgaggtat ccggcaggcc catcggattg cggtagtaca acagcttgcg
     1681 ctccttcttc gcgccctccg cggggaccgc tgcgactgag gctgccggcg ccccatggct
     1741 cgccatgggg cgctgcccga accagtaacc gccgccggca gccaacgtga cagcaaggcc
     1801 gaccgcaatg cctgcgcctg ttttcatagg tcttctccaa gaatgcgttc gatgtcggca
     1861 aggcgtatct gtgcttcggc ctgggccttg atttgggact gtcgggcctg ccggatctgc
     1921 cgctgggcgt cgagcaaagt ggcgaagtcc accttgccgg tctcgtaccc ggcgagggcc
     1981 gccttgaaag tgagttcaga ttgcggcaag aggctcgttg aggccaggtt ctcggtccgg
     2041 cgagccgcat cgaaaccgga caggttctcg gccagatcac ccagcacctg attggcggtc
     2101 gcgtcgcggc gggccttggc ggcagacagc atggcctcgg actcccgctc ctgggcccgg
     2161 cggctgccct gctgtagcgg caggttcagt tccaccatca attcccattc acgcaccgcg
     2221 ccctggtact ggattggcga gacaccgagc gtgaagtcgg ggtagcggtt cttgtaagtg
     2281 agttcgcgac tcttctccgc cgagcgaatg cgggcttcgt cggcaaagat ctgtgggttc
     2341 ttcgtccgtg cccgctcttc cagggtcgtc ggatcgagtt gagcgggcgc gggcagtgtc
     2401 cgcaggcgct ccggggccgc caagggagag gaagtcggcc ttgcaagaag ggcattcagg
     2461 cgcgcctgca gttgccgacg ttcgttctgc agggcgacca actcattttt catgctggtc
     2521 tgttcgacct gggcacggat cacgtcctgt tgagcggcca gtccgccggc gtagcgcacc
     2581 tgggcgatct tctcgagacg gaccatcagg tcgaggattt cgcgggtcag ctgctcgttg
     2641 cgatagacgt actggagc
//
LOCUS       DAOQNN010000432         2654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_29187_length_2654_cov_9.517891, whole genome shotgun sequence.
ACCESSION   DAOQNN010000432 DAOQNN010000000
VERSION     DAOQNN010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29187_length_2654_cov_9.517891"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /gene="ychF"
                     /locus_tag="PKV97_15720"
     CDS             complement(<1..970)
                     /gene="ychF"
                     /locus_tag="PKV97_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNB07383.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIQAENYPFCTIEPNVGIV
                     EVPDPRLAALSEIVKPQKIQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVH
                     VVRCFADDNVIHVSGSVDPIRDIEVIDTELALADMATVEKALNRYKRPAASGDKEAKI
                     LVAVLEKCFAQLDQGKAVRALDLSKEEWASLKPFCLITAKPVLYAANVAEDGFDNNPH
                     LDAVRAHAAAEGAEVVALCAAIEAEIADLEDADKKEFLETMGLEEPGLDRLIRAGYTL
                     LGLQTYFTAGVKEVRAWTIHVGDTAPQAAGVIHTDFERGFIRAQTIA"
     gene            1399..2373
                     /locus_tag="PKV97_15725"
     CDS             1399..2373
                     /locus_tag="PKV97_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HNB07384.1"
                     /translation="MSINWIEAIDACSNAALASGALVPVQAEQVEMEDAGMRFILRWV
                     SSLAAKDASRSAAPAQADAAGDRAGKAEGVQGGVVNDKVTKDDKAVAIPGGPRDPNFN
                     PFLNPDPALTVGRVGDAHVAILNKFPLCARHLVLARREFEEQLRPLARSDFAALAELM
                     GEAGGIGLYNGGTAAGASQRHKHVQWMPDDAGNASLRLFAPGLPAGLAEQGMATHPAL
                     PMRHCFVRVRCGRGEAVETAADSLQAGFERACAELGLGAGEDGLLPPFNLLAGDGWLL
                     AIPRSREHFEGISMSAVCFGGTLYTRHREQIEAIRAVGPLRALAAVGC"
     gene            complement(2380..>2654)
                     /locus_tag="PKV97_15730"
     CDS             complement(2380..>2654)
                     /locus_tag="PKV97_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNB07385.1"
                     /translation="RIGIGHPGDRNEVVNFVLKPARREEQQQIDEALDKALAVWPQIV
                     RGELNAAATRLNVRPAPPKPPKPPKTPRPAQPDAGMEPPPTDGSAA"
BASE COUNT          354 a          819 c         1042 g          439 t
ORIGIN      
        1 aggcgatggt ctgggcgcgg atgaagccgc gctcgaagtc ggtgtggatg acgccggcgg
       61 cctgcggcgc ggtgtcgccg acgtggatgg tccacgcgcg cacttccttg acgccggcgg
      121 tgaagtaggt ctgcaggccg agcagcgtgt agccggcgcg gatcaggcgg tcgaggccgg
      181 gctcttcgag gcccatggtc tcgagaaact ccttcttgtc ggcgtcctcg aggtcggcga
      241 tctcggcctc gatcgcggcg cacagcgcga ccacttcggc gccctcggcg gcggcgtggg
      301 cgcgcacggc gtcgaggtgc gggttgttgt cgaagccgtc ctcggcgacg ttggcggcgt
      361 agagcaccgg cttggcggtg atcaggcaga agggcttgag gctggcccat tcttccttcg
      421 acaggtcgag cgcgcgcacg gccttgccct ggtcgagctg ggcgaagcac ttctcgagca
      481 cggcgacgag gatcttggcc tccttgtcac ccgaggcggc agggcgcttg tagcggttga
      541 gcgccttctc gacggtggcc atgtcggcga gggcgagctc ggtgtcgatg acctcgatgt
      601 cgcggatcgg gtcgacactg ccggagacgt ggatcacgtt gtcgtccgcg aagcagcgca
      661 cgacgtgcac gatggcgtcg gtctcgcgga tgttggcgag gaactggttg cccaggcctt
      721 cgcccttgga ggcgccggca accaggccgg cgatgtcgac gaactcgacg atggcgggct
      781 ggatcttctg cggcttgacg atctcggaca gcgcggccag gcgcgggtcc ggcacctcga
      841 cgatgccgac gttgggctcg atggtgcaga aggggtagtt ctcggcctgg atgccggcct
      901 tggtcagcgc gttgaagagg gtcgacttgc cgacgttggg caggccgacg attccgcatt
      961 tcaggctcat gtgaatcctt gatcgttccg ggggctccgt cgccgcatcg ggggctacgg
     1021 ccttgatccg gtatggaaag ccggggattg taccgtgtcg cggggtaggg ctccaaagcg
     1081 ggcgcggggt cggtccgtgc gcggactgcg ggctagggcg gcggtgcggc ggtggggggc
     1141 gcggttgggg gcggcttcga tccgaccgcg gggccggggc ggggcacgat ctcttggtgc
     1201 atttccgcag ccgtgccggt ttccgtagga gcgacgcgag tcgcgatcgc cacctccgca
     1261 tccaccgtgg cctggccgac cgcgtcgtcg cggctcctgc ggccatcctc gccatttccg
     1321 gccaggagcc attcggttcg ctgccggcct acaatcgccc atcctgcatt tgcccaaaaa
     1381 cacaccggag gagaagggat gagcatcaac tggatcgagg cgatcgatgc gtgcagcaac
     1441 gcggcgctgg cgtcgggggc gctggtgccg gtgcaggccg agcaggtcga aatggaagac
     1501 gcgggcatgc gcttcatcct gcgctgggtg tcctcgctcg cggccaagga tgcgagcagg
     1561 agcgccgcgc cggcgcaggc cgacgccgct ggcgacaggg cgggcaaggc cgaaggcgtg
     1621 caaggtgggg tggtcaatga caaggtgacc aaggacgaca aggcggtggc gatccccggc
     1681 gggccgcgcg atccgaactt caatcctttc ctgaatcccg acccggcgct caccgtcgga
     1741 cgggtgggtg atgcccatgt ggcgatcctg aacaagttcc ccctgtgcgc gcgccacctg
     1801 gtgctggcgc ggcgggagtt cgaggagcag ctgcggccgc tcgcgcgcag cgactttgcc
     1861 gcgctcgccg agctgatggg cgaggcgggc gggatcgggc tctacaacgg cggcaccgct
     1921 gcgggggcga gccagcgcca caagcacgtg cagtggatgc ccgacgatgc cggcaacgcc
     1981 agcctgcggc tgttcgcgcc cgggctgccc gcagggctgg cggagcaggg gatggcgacg
     2041 catccggcgc tgccgatgcg gcattgcttc gtgcgcgtgc gctgcggtcg cggcgaggcg
     2101 gtggagactg cggccgacag cctgcaggcc ggcttcgagc gtgcgtgtgc cgagctcggc
     2161 ctgggagcgg gggaggacgg gctgctgcca cccttcaacc tgctcgcggg cgatggctgg
     2221 ctgctggcga ttccgcgcag ccgggagcac ttcgagggga tctcgatgag cgcggtgtgc
     2281 ttcggcggca cgctgtacac acgccatcgc gagcagatcg aggcgatccg cgcggtcggg
     2341 ccgctgcgcg cgctggcggc ggtgggctgc tgagcgggat caggcggcgg agccgtccgt
     2401 cgggggcggc tccatgccgg cgtcgggttg cgcgggcctg ggggtcttgg gaggcttcgg
     2461 gggcttgggc ggcgcgggac gcacgttgag ccgggtcgcc gcggcattga gttcgccgcg
     2521 cacgatctgc ggccagaccg cgagcgcctt gtcgagcgct tcgtcgatct gctgctgttc
     2581 ttcgcgccgc gcgggcttga gcacgaagtt caccacctcg ttgcggtcgc ccggatggcc
     2641 gatgccgatg cgca
//
LOCUS       DAOQNN010000433         2649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_29283_length_2649_cov_11.005012, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000433 DAOQNN010000000
VERSION     DAOQNN010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29283_length_2649_cov_11.005012"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /gene="truB"
                     /locus_tag="PKV97_15735"
     CDS             complement(<1..752)
                     /gene="truB"
                     /locus_tag="PKV97_15735"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNB07386.1"
                     /translation="MTLAPAAEVAAEPARPKRKGQPQRRIVRREVDGVLLLDKPQGMS
                     SNGALQTARRLLAAAKAGHTGTLDPMASGLLPLTFGEATKFSQMLLDADKTYEAVVRL
                     GVDTDSGDAEGKVLATRPVAVDRAALEAVLERFRGEIEQVPPMYSALKRDGKALYEYA
                     RAGIELERAARRVVIHALELLDFAGERFTIRVHCSKGTYIRSLAMDIGAALGCGAHLA
                     ALRRTAIGAFDLSGALTLEALEAAGEGGRDALL"
     gene            complement(730..1155)
                     /gene="rbfA"
                     /locus_tag="PKV97_15740"
     CDS             complement(730..1155)
                     /gene="rbfA"
                     /locus_tag="PKV97_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNB07387.1"
                     /translation="MPKEYSRSQRVVEQIRRELAELIRLEVKDPRVGFITLTDVEITP
                     DYAHAKVFFTNMTGEADVPEILQGLRRASGFLRRELGRRIRIHTIPELHFHYDRSVEE
                     GSRLSQLIDEVVKEDEARQHDEAGGDDNTGTDDDARSRG"
     gene            complement(1159..>2649)
                     /gene="infB"
                     /locus_tag="PKV97_15745"
     CDS             complement(1159..>2649)
                     /gene="infB"
                     /locus_tag="PKV97_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNB07388.1"
                     /translation="VTVMGHVDHGKTSLLDYIRRAKVAAGEFGGITQHIGAYHVETAR
                     GMLTFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTREAIHHAKAAGVPLVV
                     AINKIDKPDANPERVTQELIAESVIPEAYGGDTMFVPVSAKKGTGIDELLEAVLLQAE
                     VLELTAPKDTPAKGLIIEARLDKGRGPVASLLVQSGTLRKGDVLLAGATFGRIRAMLD
                     ENGKQIDEAGPSIPVEILGLSDVPSAGDEAIALADEKKAREIALFRQGKFRDVKLAKQ
                     QAAKLETLMEQMSEGEVKSLALIIKADVQGSQEALVQSLQKLSTDEVRVNVIHGAVGA
                     ISESDVNLAQASGAVIIGFNTRADAGARKLAETFGVDIRYYNIIYDAVDEVKAALSGM
                     LAPEKREEVTGLVEIRQVFTISKVGSIAGCYVLEGVVRRNSHVRLLRNHTVLWTGELE
                     SLKRFKDDVKEVKFGYECGLQLRNYNDIQEGDQLEVFEIKEVARTL"
BASE COUNT          376 a          918 c          895 g          460 t
ORIGIN      
        1 agcagcgcat cgcgtccgcc ctcgccggcc gcctccagcg cttcgagcgt gagcgcaccg
       61 gacaggtcga aggcgccgat cgcggtgcgc cgcaacgccg ccaggtgggc accgcaaccg
      121 agcgcagcgc cgatgtccat cgcgaggctg cggatgtagg tgcccttgct gcaatgcacg
      181 cggatcgtga agcgttcgcc tgcgaagtcg agcagttcga gcgcatggat cacgacccgg
      241 cgcgccgcgc gctcgagctc gatgcccgcg cgcgcgtact cgtagagcgc cttgccgtcg
      301 cgcttgagcg ccgaatacat cggcggcacc tgctcgatct cgccgcggaa gcgctccagc
      361 acggcttcga gcgcggcgcg atccaccgcc accggacggg tggcgagcac cttgccctcg
      421 gcatcgccgc tgtcggtgtc cacgccgagc cgcaccaccg cctcgtaggt cttatcggcg
      481 tcgagcagca tctgcgagaa cttggtggcc tcgccgaagg tgagcggcag cagtccgctc
      541 gccatcggat ccagcgtgcc ggtgtggccg gccttggccg ccgcaagcag gcgccgtgcg
      601 gtctgcaagg caccgttcga gctcatgccc tgcggcttgt cgagcaacag cacgccgtcg
      661 acttcgcggc ggacgatcct gcgttgcggc tggcccttgc gcttcggccg cgccggctca
      721 gccgcgacct cagccgcggg agcgagcgtc atcatccgtg ccggtgttgt cgtcgccgcc
      781 agcctcgtcg tgctggcggg cctcgtcttc cttcaccacc tcgtcgatca gctgcgacag
      841 gcgcgagccc tcttccaccg aacggtcgta gtggaagtgc agctccggga tggtgtggat
      901 gcggatgcgg cggccgagct cgcgacgcag gaagccgctc gcgcggcgca agccctgcag
      961 gatctcgggc acgtcggcct cgcccgtcat gttggtgaag aacaccttgg cgtgggcgta
     1021 gtcgggggtg atctcgacat cggtcagggt gatgaagccg acgcgcgggt ccttcacctc
     1081 gaggcggatc agctcggcca gctcgcggcg gatctgctcc accacgcgct ggctgcggga
     1141 atactccttg ggcatcgctt acagggtcct cgccacttcc ttgatctcga agacctcgag
     1201 ctggtcgcct tcctggatgt cgttgtagtt gcgcagctgc aggccgcact cgtagccgaa
     1261 cttgacttcc ttgacgtcgt ccttgaagcg cttgagcgac tcgagctcgc cggtccacag
     1321 cacggtgtgg ttgcgcagca ggcggacgtg cgaattgcgg cgcacgacac cctcgagcac
     1381 gtagcaaccc gcgatcgagc cgaccttgga gatcgtgaac acctggcgga tctcgaccag
     1441 gccggtgact tcctcgcgct tctccggcgc cagcatgccc gacagcgccg ccttcacctc
     1501 atcgacggcg tcgtagatga tgttgtagta gcggatatcg acaccgaagg tctcggccag
     1561 cttgcgcgcc ccggcatcgg cacgggtatt gaagccgatg atgaccgcgc ccgaggcctg
     1621 cgccaggttg acgtcggatt cgctgatcgc gcccaccgca ccgtggatga cgttgacgcg
     1681 gacttcgtcg gtggagagct tctgcagcga ctgcaccagc gcctcctgcg aaccctgcac
     1741 gtcggccttg atgatgagcg ccaggctctt gacctcgccc tcggacatct gctccatgag
     1801 cgtctcgagc ttggcggcct gctgcttggc gagcttgacg tcgcggaact tgccctggcg
     1861 gaagagcgcg atctcgcgcg ccttcttctc gtcggccagc gcgatcgcct cgtcacccgc
     1921 ggacggcacg tccgacaggc cgaggatctc gaccgggatg gacggaccgg cctcgtcgat
     1981 ctgcttgccg ttttcgtcca gcatcgcgcg gatgcggccg aaggtggcgc cggcgagcag
     2041 cacgtcgccc ttgcgcagcg ttcccgactg gaccagcagc gaggccaccg ggccacgacc
     2101 cttgtccagg cgcgcctcga tgatcaggcc cttggcgggc gtgtccttgg gcgcggtgag
     2161 ctcgagcacc tcggcctgca gcagcacggc ctcgagcagc tcgtcgatgc cggtcccctt
     2221 cttggcggac accggcacga acatggtgtc gccgccgtag gcctcgggga tcaccgactc
     2281 ggcgatcagc tcctgggtca cgcgctcggg gttggcgtcg ggcttgtcga tcttgttgat
     2341 cgccaccacc agcggcacgc cggccgcctt ggcgtggtgg atcgcctcgc gcgtctgcgg
     2401 catcacgccg tcgtcggccg cgaccaccag gatgacgatg tcggtcgcct tcgccccgcg
     2461 ggcacgcatc gccgtgaagg cctcgtggcc cggggtgtcg aggaacgtga gcatgccgcg
     2521 cgcggtctcg acgtgatacg cgccgatgtg ctgggtgatg ccgccgaact cgcccgcggc
     2581 gaccttggcg cggcggatgt agtcgagcag cgaggtcttg ccgtggtcga cgtggcccat
     2641 cacggtgac
//
LOCUS       DAOQNN010000434         2647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_29321_length_2647_cov_9.686728, whole genome shotgun sequence.
ACCESSION   DAOQNN010000434 DAOQNN010000000
VERSION     DAOQNN010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29321_length_2647_cov_9.686728"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PKV97_15750"
     CDS             <1..951
                     /locus_tag="PKV97_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB07389.1"
                     /translation="LRPGYAIEYDYFDPRNLKSSLETKSIGGLFFAGQINGTTGYEEA
                     AAQGLLAGANAALQVQGREPWCPRRDEAYLGVLVDDLITRGVSEPYRMFTSRAEYRLS
                     LREDNADLRLTEKGRELGLVDDERWAAFCAKREAIERGTAQLKAAWARPEAIPADDQQ
                     RVLGKALEREQRYFELLRRPETSYASLMSLPGAPDERETDAQVIEQIEIAAKYQGYID
                     RQQDEVTKQMQAEATRLPVGLDYAQVRGLSKEVQQKLNQHKPETIGQAGRIQGVTPAA
                     ISLLLVWLKRRDLAARAGAVAPELAAPADAGDDVARRPAA"
     gene            1031..1684
                     /gene="rsmG"
                     /locus_tag="PKV97_15755"
     CDS             1031..1684
                     /gene="rsmG"
                     /locus_tag="PKV97_15755"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNB07390.1"
                     /translation="MSGRLQPYAARLADGVAALGLALPQETIDRLLAFGELLLKWNKV
                     YNLTAIRSPQELITHHLLDSLAVLPQLEAVNRLADIGSGGGLPGVVLAIVRPGLVVSS
                     IETVGKKASFQQQAKIELGLDNFSVLNQRVEQVRPQSLPGGAPEGVISRAFSSLADFV
                     ALSGHLVAEGGALYAMKGVNPVDEIAALPAGWAVAETHALTVPGLGAERHLLVIRRA"
     gene            1792..2562
                     /locus_tag="PKV97_15760"
     CDS             1792..2562
                     /locus_tag="PKV97_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNB07391.1"
                     /translation="MAKIFCVANQKGGVGKTTTCVNLAAALHQAGQRVLLIDLDPQGN
                     ATMGSGVDKRDLKSSVYHLLVGMVDLAGVRVASPTGGYDVLPANRDLAGAEVELVNLD
                     QREKRLRNALAAFVDDYDFVLIDCPPSLSMLTLNGLCCATGVIIPMQCEYYALEGLSD
                     LVNTIKKVHANLNRDLKIIGLLRVMFDPRVTLQQQVSNQLEGHFGDKVFRAIVPRNVR
                     LAEAPSHGMPGVVFDKAAKGAQAYMAFAHEMIERAKTF"
BASE COUNT          437 a          915 c          920 g          375 t
ORIGIN      
        1 ctgcgccccg gctacgccat cgagtacgac tacttcgacc cgcgcaacct caagtccagt
       61 ctggagacca agtcgatcgg cgggctgttc ttcgccggcc agatcaacgg caccaccggc
      121 tacgaggagg ccgccgcgca gggcctgctc gcaggcgcca acgccgcgct gcaggtgcag
      181 ggccgcgagc cctggtgtcc gcgccgcgac gaggcctacc tcggcgtgct ggtggacgac
      241 ctgatcacgc gtggcgtgtc cgagccctac cgcatgttca cctcgcgcgc cgagtaccgg
      301 ctgtcgctgc gcgaggacaa cgccgacctg cgcctgaccg agaagggccg cgaactcggc
      361 ctggtggacg acgaacgctg ggccgccttc tgcgccaagc gcgaggcgat cgagcgtggc
      421 acggcgcagc tcaaggcggc gtgggcgcgg ccggaggcga ttcccgccga cgaccagcag
      481 cgcgtgctcg gcaaggcgct cgagcgcgag cagcgctact tcgagctgct gcgccggccg
      541 gagaccagtt acgcctcgct gatgagcctg cccggcgcgc cggacgagcg cgagaccgac
      601 gcgcaggtga tcgagcagat cgagatcgcc gccaagtacc agggctacat cgaccgccag
      661 caggacgagg tcacgaagca gatgcaggcc gaggccacgc gcttgccggt tggcctcgac
      721 tacgcccagg tgcgcggcct gtccaaggaa gtgcagcaga agctcaacca gcacaagccc
      781 gagaccatcg gccaggccgg ccgtatccag ggcgtgaccc cggcggcgat ctcgctgctg
      841 ctggtgtggc tcaagcgccg cgacttggcg gcgcgcgcgg gcgctgtggc gcccgagctt
      901 gcggcgcctg cggacgcggg cgacgacgtg gcacggaggc cggcggcatg agcgcgctcg
      961 tatggcggcc ggaaggcaag cgcaccgctt ccatggggaa gagctcgcac cgcgacggga
     1021 gagaattgca gtgagcgggc gcctgcagcc ttacgccgcc cggctcgccg acggcgtcgc
     1081 cgcgctcggc ctcgccctcc cgcaggagac catcgaccgc ctgctcgcct tcggcgagct
     1141 gctgctgaag tggaacaagg tctacaacct cacggcgatc cgctcgccgc aggagctgat
     1201 cacccatcac ctgctcgact cgctcgcggt gctgccgcag ctggaggcgg tgaaccggct
     1261 cgccgacatc ggctcagggg gcggcctgcc gggcgtggtg ctggcgatcg tgcgccccgg
     1321 gctggtggtg tcctcgatcg agaccgtggg caagaaggcg agcttccagc agcaggcgaa
     1381 gatcgagctc gggctggaca acttcagcgt gctgaaccag cgcgtcgagc aggttcggcc
     1441 acagtcgctg cccggcggcg cgccggaagg cgtgatctcg cgggccttct ccagcctggc
     1501 ggatttcgtc gcgctgtccg gccacctcgt cgccgagggc ggcgcgctgt acgcgatgaa
     1561 aggcgtgaat ccggtcgacg agatcgccgc gctgcctgcg ggctgggcgg tggcggagac
     1621 ccatgcgctc acggtgccgg ggctgggcgc cgagcgtcat ctgctggtca tccggcgggc
     1681 gtgacactgc ggccctgaga agcgtcgtgc atgctgcatc gccgcattga agtacactcc
     1741 agggtttgca ccgtgtgtgc gagccttcca accggaacag cctgaaccga catggccaag
     1801 atattctgcg tcgccaacca gaagggcggg gtgggcaaga ccaccacctg cgtcaatctc
     1861 gccgccgccc tgcaccaggc cgggcagcgc gtgctgctga tcgacctcga tccgcagggc
     1921 aacgccacca tgggcagcgg ggtcgacaag cgcgacctga agagttcggt gtatcacctg
     1981 ctggtgggca tggtcgacct cgccggggtg cgggtggcct cgcccaccgg cggctacgac
     2041 gtgctgccgg ccaatcggga cctcgccggc gccgaggtcg agctggtcaa cctcgaccag
     2101 cgcgagaagc gcctgcgcaa cgcgctcgcc gccttcgtcg acgactacga cttcgtcctc
     2161 atcgactgcc cgccctcgct gtcgatgctg accctcaacg gcctgtgctg cgccaccggg
     2221 gtgatcatcc cgatgcagtg cgagtactac gcgctcgagg ggctttcgga cctggtcaac
     2281 acgatcaaga aggtgcatgc caacctcaac cgcgacctca agatcatcgg cctgctgcgg
     2341 gtgatgttcg acccgcgcgt gaccctgcag cagcaggtct cgaaccagct cgagggtcac
     2401 ttcggcgaca aggtcttccg cgccatcgtg ccgcgcaacg tgcgcctggc cgaggcgccc
     2461 agccacggca tgccgggcgt ggtgttcgac aaggcggcca agggtgccca ggcctacatg
     2521 gccttcgcgc acgagatgat cgagcgcgcg aagaccttct gaattccgga gccgacatga
     2581 acaaacccag actcaagggc ctcggccgcg gactcgacgc gctgctggcc gcgaaccagg
     2641 aagagga
//
LOCUS       DAOQNN010000435         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_29341_length_2646_cov_10.640679, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000435 DAOQNN010000000
VERSION     DAOQNN010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29341_length_2646_cov_10.640679"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..409
                     /locus_tag="PKV97_15765"
     CDS             20..409
                     /locus_tag="PKV97_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HNB07392.1"
                     /translation="MIGLFLVTHGTLGEALIQCVCHVLDKRPPLLGQLGLSAGEDPAD
                     ALPLARRMIATVDDGEGVLLLADLYGATPCNVATRLIEPGRIELVAGVSLPMLLRLLT
                     HRDGNLDTLIRRAVSGAGDGVTRVRPG"
     gene            463..732
                     /locus_tag="PKV97_15770"
     CDS             463..732
                     /locus_tag="PKV97_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNB07393.1"
                     /translation="MPRGDAPIINKLGLHARASAKLTQLASSFPCEVWLERNGRRVNA
                     KSIMGVMMLAAARGSTITIDTQGEGADAALQAIQELVADRFGEGE"
     gene            734..2464
                     /gene="ptsP"
                     /locus_tag="PKV97_15775"
     CDS             734..2464
                     /gene="ptsP"
                     /locus_tag="PKV97_15775"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNB07394.1"
                     /translation="MSFTLHGLAVSQGIAIGHVHLVSHALLEVNHYHVAPRYLDDELA
                     RLDEALATVQGELIGLKAITTSGQAHSEVGAFVDLQLMMLADPMLVDAARQLVTERRC
                     NAEWALVQQMEHLVEQFRQIEDPYLRERQADVVQVVERLVKVLLGHPGHLPPRRRDGL
                     GSIVVAHDLSPADTIGFRDHNIAGFVTDVGGPTSHTAIVARSLKIPAVVGLHHVRDLL
                     EDDELIIVDGTRGVIIVAPDEQIVEEYRLRRSELEIERSKLNRLRDTRAATLDGEEVN
                     LLANIEGPKDLPAVKSANADGIGLYRTEFLFIGRDTLPDEEEQYEAYRAVVKALPGKP
                     VTIRTFDVGADKALNGAQAREEPNPALGLRAVRYSLAEPKMFKTQLRALLRASAHGRL
                     QIMVPMLAHAQEVDQCLTLLEKAKAELRAEKIKFDEGIRIGGMIEVPAAALSLGMFIR
                     RLAFLSIGTNDLIQYTLAIDRSDEAVAHLYDPLHPAVLKLIAGTISSGARFGLPVSVC
                     GEMAGDPLYTELLLGMGLRNFSMHPGSILEIKQQVLRADLGELAPRVQRILKMDEPAR
                     IREAVERLTA"
     gene            2547..2621
                     /locus_tag="PKV97_15780"
     tRNA            2547..2621
                     /locus_tag="PKV97_15780"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2580..2582,aa:Gly,seq:ccc)
BASE COUNT          445 a         1003 c          825 g          373 t
ORIGIN      
        1 gaaggctgcg gagaacccca tgatcggcct ctttctcgtc acccacggca ccctcggcga
       61 agcgctgatc cagtgcgtgt gccacgtcct cgacaagcgc ccgccgctgc tcggccagct
      121 cggcctgtcg gcgggcgagg atcccgccga cgcgctgccc ctcgcgcgcc ggatgatcgc
      181 cacggtcgat gacggcgaag gcgtgctgct gctcgccgac ctctacggcg ccaccccgtg
      241 caacgtcgcc acgcgcctga tcgagcccgg gcgcatcgag ctcgtcgccg gagtcagcct
      301 gccgatgctg ctgcgcctgc tgacccatcg cgacggcaac ctcgacaccc tgatccggcg
      361 cgccgtcagc ggcgcgggcg acggcgtgac gcgcgtgcgg cccggctgat cacacccgcc
      421 aacaagaaca tacgaacgaa cgcaccacgg agaaccgcga agatgccgcg aggagacgcc
      481 cccatcatca acaagctcgg tctgcacgcg cgcgcctcgg ccaagctcac ccagcttgcc
      541 agcagctttc cgtgcgaggt ctggctcgag cgcaacggcc gccgggtgaa cgcgaagagc
      601 atcatggggg tgatgatgct ggcggccgcg cgcggcagca cgatcaccat cgacacccag
      661 ggcgagggcg ccgacgcggc actgcaggcc atccaggagc tggtcgccga ccgcttcggc
      721 gaaggggaat gagatgtcat tcacgctgca cggcctggcg gtctcgcagg gcatcgcgat
      781 cggccatgtc cacctcgtct ctcacgcgct gctcgaggtc aatcactacc acgtcgcgcc
      841 gcgctacctc gacgatgaac tcgcccgcct cgacgaggcc ctcgccaccg tccagggcga
      901 gctgatcggt ctcaaggcga tcaccacctc cggccaggcg cacagcgagg tcggcgcctt
      961 cgtcgatctg cagctgatga tgctcgccga cccgatgctg gtcgacgccg cccgccagct
     1021 cgtcaccgag cgccgctgca acgccgagtg ggcgctggtg cagcagatgg agcacctagt
     1081 cgagcagttc cgccagatcg aggaccccta cctgcgcgaa cgccaggccg atgtcgtcca
     1141 ggtcgtcgag cgcctggtca aggtgctgct cggccacccc ggccacctgc cgccgcgtcg
     1201 gcgcgacggg ctgggcagca tcgtcgtcgc ccacgatctg tcgcccgccg acaccatcgg
     1261 cttccgcgac cacaacatcg ccggcttcgt aaccgacgtc ggcggcccca ccagccacac
     1321 cgcgatcgtc gcgcgcagcc tcaagatccc cgcggtggtc ggcctgcacc acgtgcgcga
     1381 cctgctcgag gacgacgagc tcatcatcgt cgacggcacc cgcggcgtga tcatcgtcgc
     1441 ccccgacgag cagatcgtcg aggaataccg cctgcgccgc agcgagctcg agatcgagcg
     1501 ttccaagctc aaccgcctgc gcgacacccg cgccgccacg ctcgacggcg aggaggtcaa
     1561 cctgctcgcc aacatcgagg gccccaagga cctgcccgcg gtcaagagcg ccaacgccga
     1621 cggcatcggc ctgtaccgca ccgagttcct cttcatcggc cgcgacaccc tgcccgacga
     1681 ggaagagcag tacgaggcct accgcgccgt ggtcaaggcc ctgcccggca agccggtcac
     1741 catccgcact ttcgacgtcg gcgccgacaa ggcgctcaac ggtgcccagg cgcgcgagga
     1801 acccaacccg gcgctcggcc tgcgcgcggt gcgctactcg ctggccgagc ccaagatgtt
     1861 caagacgcag ctgcgcgcgc tgctgcgcgc ctcggcccac ggccgcctgc agatcatggt
     1921 gccgatgctc gcccacgccc aggaggtcga ccagtgcctg accctgctcg agaaggccaa
     1981 ggccgagctg cgcgccgaaa agatcaagtt cgacgagggc atccgcatcg gcggcatgat
     2041 cgaggtgccc gccgcggcgc tgtcgctcgg catgttcatc cggcggctgg ccttcctgtc
     2101 gatcggcacc aacgacctca tccagtacac cctggcgatc gaccgctcgg acgaggccgt
     2161 cgcgcacctc tacgacccgc tccaccccgc cgtgctcaag ctcatcgccg gcaccatctc
     2221 cagcggcgcg cgcttcggcc tgccggtgtc ggtgtgcggc gagatggcgg gcgacccgct
     2281 gtacaccgag ctgctgctcg gcatgggcct gcgcaacttc tcgatgcacc cgggcagcat
     2341 cctcgagatc aagcagcagg tgctgcgcgc cgacctcggc gagctcgccc ccagggtgca
     2401 gcgcatcctc aagatggacg agccggcacg catccgcgag gcggtggaac ggctcaccgc
     2461 ctgaaaacac cgccgcggtt gaagcgcacc ggccgtggca gtacaatccg gccttcttcg
     2521 cggtcgacgc caggtcagcc cgaaatgcgg gtgtcgtata atggctatta cctgagcttc
     2581 ccaagctcat gacgagggtt cgattccctt cacccgctcc accgcatcat tctgcggact
     2641 tccgca
//
LOCUS       DAOQNN010000436         2637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_29507_length_2637_cov_6.461658, whole genome shotgun sequence.
ACCESSION   DAOQNN010000436 DAOQNN010000000
VERSION     DAOQNN010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29507_length_2637_cov_6.461658"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1626
                     /locus_tag="PKV97_15785"
     CDS             <1..1626
                     /locus_tag="PKV97_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB07395.1"
                     /translation="PPVPRLFEALEAVLAPLDRLPRPLWLGGMTHSGGELAGRLAALE
                     AWRAALLAGGLPGSCAAWPEAEVQEAVREVFRGLGLPAYCADQPALVDTVLQGLLFHL
                     DLIVDYRDRGDTEAAAQARALDSFAADWAERCGEIDELVGAFGDLGDLLDNARWDALR
                     GLLRSTDWREVLRIRALIEGLPELARILRALGRACPTDEDAESSRALHAVVEHTEIQR
                     SVSHRVRVPDLPGETRGVQRSGRIARMLPAEATLLGHPRLRLVWHARRAERTLLAYED
                     DDHLQEDCLRPAPVLRPSQRPAPARRLEQGPMLVCVDTSGSMQGGAEAVAKAVVLEAV
                     RCAHARRRACRVYAFGGPDEVVEMELGVDVDGVGRLARFLGQGFGGGTDICAPLERAL
                     ARLDEAGWNLADLLIASDGEFGATPALAARVEAARRERGLRVQGILIGDRETIGLLEL
                     ADDIHWVRDWRRYGGDTDKPGGADADKRRGDGAGANAPTLAAGGGSPVHSSHLTADYF
                     PGALRTPENRAATVTPEAAAAAIRAGRHRGDRL"
     gene            1778..2623
                     /locus_tag="PKV97_15790"
     CDS             1778..2623
                     /locus_tag="PKV97_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNB07396.1"
                     /translation="MSFATDLIAELERATAGCPIPRVRALHLPPPEAAASKNGEFCAL
                     ELDDGALGLSYVLLGGILPRLAASDDPHTIVGMDALQLAREFAAPAAGAGGDAEIRRT
                     LGFAAANALSRTVMERIGFAPPRATDSIGGIDPQAGDHVGMVGLFTPLLKQVTAAGAQ
                     LTVIELNPDYAGHYDGYRVTLDAAALEDCNKVLSTSTILLNHTVDDMLAHCRNARRIV
                     LIGPSAGCLPDPLFARGVTVVGGSWITDRAGFIDALRGGESWSGFAYKFALGAADWPG
                     LPENR"
BASE COUNT          314 a         1022 c          936 g          365 t
ORIGIN      
        1 ccacccgtcc cccggctctt cgaggcgctc gaagccgtgc tcgcgccgct cgatcgcctg
       61 ccgcgtccgc tctggctcgg tggcatgacc cactccggcg gcgaactcgc gggccgtctc
      121 gccgcgctcg aagcctggcg ggctgcgctg ctcgccggcg gcctgcccgg gtcttgcgcg
      181 gcctggcccg aggcggaggt gcaggaggcc gtgcgcgagg tcttccgggg cctgggcttg
      241 cccgcctact gtgcagacca gcccgcgctg gtcgataccg tgctgcaagg cctgctcttc
      301 cacctcgacc tgatcgtcga ctaccgcgac cgcggcgata ccgaggccgc ggcgcaggcg
      361 agggcgctcg attccttcgc tgccgactgg gccgagcgct gcggcgagat cgacgagctg
      421 gtcggcgcct tcggggatct cggcgatctg ctcgacaacg cccgctggga tgcgctgcgc
      481 ggcctgctgc gcagcacgga ctggcgcgag gtgctgcgca tccgcgcgct gatcgagggc
      541 ctgcccgagc tcgcccgcat cctgcgcgca ctcggccgcg cctgccccac cgacgaggac
      601 gccgaatcca gccgggcatt gcacgccgtg gtcgagcaca ccgagataca gcgcagcgtc
      661 tcgcaccggg tgcgggtgcc cgacctgccc ggcgaaacgc gcggcgtgca gcgctcgggc
      721 cggatcgcgc gcatgctgcc ggccgaggcg acgctgctcg gccatccgcg cctgcgcctg
      781 gtctggcatg cgcgccgcgc cgagcgcacg ctgctcgcct acgaggacga cgaccacctg
      841 caggaggact gcctgcgccc ggcgccggtg ctgcgcccca gccagcgccc cgcgccggca
      901 cggcgcctgg agcaggggcc gatgctggtg tgcgtggata cctcgggctc gatgcagggg
      961 ggcgccgagg cggtggccaa ggcggtcgtg ctggaggcgg tgcgctgcgc tcacgcccgg
     1021 cgccgcgcct gccgggtgta tgccttcggc gggcccgacg aggtggtcga gatggagctc
     1081 ggcgtcgatg tcgatggcgt cggccggctc gcccgcttcc tcggccaggg cttcggcggc
     1141 ggcaccgaca tctgcgcccc gctcgagcgt gcgctcgccc gcctcgacga agccggctgg
     1201 aacctcgccg acctgctgat cgcgtccgat ggcgagttcg gcgccactcc ggcgctcgcg
     1261 gcgcgcgtcg aggccgcccg ccgcgagcgt ggcctgcgcg tgcagggcat cctgatcggc
     1321 gaccgcgaga ccatcggcct gctcgaactc gccgacgaca tccactgggt gcgcgactgg
     1381 cggcgctatg gaggcgatac ggacaagccg ggtggggccg acgcggacaa gcggcgtggc
     1441 gatggcgccg gcgcgaacgc cccgaccctt gctgccggcg gcggctcgcc ggtgcattcc
     1501 agccacctca ccgcggacta tttccccggt gccctgcgca cccccgagaa ccgtgccgcc
     1561 accgtcacac ccgaggcggc tgccgctgcc atccgtgccg gccgccaccg cggcgaccgg
     1621 ttgtaggagc ggcgcacgcc gcgaacgtcg gccttcgggc aatccccgcg cgtctcgttc
     1681 ccgcggccgg attcgcgcct tgcggcgctc ctgcaggggc gtgccgtcgt ggtaggctcg
     1741 cccgacccca ccgcccggcc ttgcgccgct gtccatcatg agcttcgcca ccgacctgat
     1801 cgccgaactc gaacgcgcca ccgccggctg cccgatcccg cgcgtgcgcg ccctgcacct
     1861 gccgccgccg gaggcggccg catcgaagaa cggcgagttc tgcgcgctcg agctcgacga
     1921 cggcgcgctc ggcctctcct atgtgctgct cggcggcatc ctcccgcggc tcgccgcctc
     1981 cgacgacccg cacaccatcg tcggcatgga cgcgctgcag ctcgcgcgcg agttcgccgc
     2041 gcccgccgcc ggcgcggggg gcgacgcgga gatccgccgc acgctcggct tcgccgcggc
     2101 caacgcgctg tcgcgcacgg tgatggagcg catcggtttc gcgccgccgc gcgccaccga
     2161 ctcgatcggc ggcatcgacc cgcaggcggg cgaccacgtc ggcatggtgg ggctgttcac
     2221 cccgctgctg aagcaggtca ccgctgccgg cgcgcagctc accgtgatcg agctgaaccc
     2281 cgactatgcc ggccactacg acggctaccg cgtcaccctc gacgccgcgg cgctggaaga
     2341 ctgcaacaag gtcctgtcga ccagcaccat cctgctcaac cacacggtgg acgacatgct
     2401 cgcgcattgc cgcaatgcgc ggcgcatcgt gctgatcggc ccgagcgccg gctgcctgcc
     2461 cgatccgctg ttcgcccgcg gcgtcaccgt ggtgggcggg agctggatca ccgatcgcgc
     2521 cggcttcatc gacgcgctgc gcggcggcga gtcctggagc ggcttcgcct acaagttcgc
     2581 gctcggcgcg gcggactggc caggcctgcc ggaaaaccgc taaggttgcg gcctcgc
//
LOCUS       DAOQNN010000437         2633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_29586_length_2633_cov_7.493794, whole genome shotgun sequence.
ACCESSION   DAOQNN010000437 DAOQNN010000000
VERSION     DAOQNN010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29586_length_2633_cov_7.493794"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PKV97_15795"
     CDS             complement(<1..903)
                     /locus_tag="PKV97_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNB07397.1"
                     /translation="MDSQTIRILLDGEAVSITGLAPTTSLLAWLRGARGLVGTKEGCN
                     EGDCGACTVVVGELADDPRDVPRLYPVNACIRFLATLDGKAVFTVEHLRSLAGGALHP
                     VQQAMVDCHGSQCGFCTPGFTMSLWALYEDRHAQGEAAADPARIRSALAGNLCRCTGY
                     RPIVEAGERMFDAPSRLLDRDRLRTAVLALRRDDTLDFSHEGGRFVAPRSVDELLALR
                     SRHPAATLLAGGTDIGLWVNKGLRRLDTLIHTGEVAALRAIVPHAGGLRIGAAASLTA
                     AFDALVRLHPELAELRERFASPPVR"
     gene            complement(985..2385)
                     /locus_tag="PKV97_15800"
     CDS             complement(985..2385)
                     /locus_tag="PKV97_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HNB07398.1"
                     /translation="MSDQTRTSRPLELDPVDQCPPGGRLFTLALQHVLVMYAGAIAVP
                     LIVGGALKLPKDQIAFLINADLLCCGLVTIIQALGVGRFGIRLPIMMGVTFAAVGPMV
                     AMAGNPALGINAILGAGIAAGLFGILFAPLVARALPLFPPVVTGSIIAVIGLSLMPVA
                     INWSAGGQPRIRDAATGLMVDNPAYGAPEYLAIAAIVLVVVLAITRYGRGFLSNVAVL
                     VGMGVGFAIAIALGKVGFDGLADAPWVSIVTPLHFGAPIFDPISIATMCLVMVVVMVE
                     SLGMFLALGELTGRQLSRDDLTRGLRTDGLGTLIGGFLNTFPHTSFSQNVGLVGVTGV
                     KSRWVCVAGGAILMAFGLFPKMALIIASVPQYVLGGAGLVMFGMVAATGIKILLAADL
                     RNNRFNLYIVALSVGAGMIPMVAPKFFQQMPHALEPLLHSGILLAAITAVTLNLFLNG
                     GRRSTTADAIEPALAH"
BASE COUNT          419 a          849 c         1024 g          341 t
ORIGIN      
        1 gcgcaccggc ggcgaggcga agcgctcccg cagctcggcc agctccgggt gcaggcggac
       61 gagcgcgtcg aaggcggccg tgagcgaagc cgctgcgccg atgcgcaggc cgccggcgtg
      121 cggcacgatc gcgcgcaggg cggcgacctc gccggtgtgg atcagggtgt cgagccggcg
      181 caggcccttg ttgacccaca ggccgatgtc ggtgccgccg gcgagcaggg tggccgcggg
      241 gtggcggctg cgcagggcga ggagttcgtc cacgctgcgc ggggcgacga agcggccgcc
      301 ctcgtggctg aaatcgagcg tgtcgtcgcg gcgcagcgcg agcacggcgg tgcgcaggcg
      361 atcgcggtcg agcaggcgcg acggggcgtc gaacatgcgc tcgccggcct ccacgatcgg
      421 ccggtagccc gtgcagcggc acaggttgcc ggcgagcgcg ctgcggatgc gggcgggatc
      481 cgcggcggcc tcgccctgcg cgtgacggtc ctcgtacagc gcccacagcg acatcgtgaa
      541 gccgggggtg cagaagccgc attgcgagcc gtggcaatcg accatcgcct gctgcaccgg
      601 gtgcagggcg ccgccggcga ggctgcgcag gtgttcgacg gtgaacaccg ccttgccgtc
      661 gagcgtggcc aggaagcgga tgcaggcgtt taccgggtag aggcgcggca cgtcgcgtgg
      721 atcgtcggcg agctcgccca ccaccacggt gcaggcgccg cagtcgccct cgttgcagcc
      781 ctccttggta ccgacgaggc cgcgcgcgcc gcgcagccag gcgagcaggc tggtggtggg
      841 ggcgaggccg gtgatcgaca ccgcctcgcc gtcgagcagg atgcgtatcg tctggctgtc
      901 catcttcggg tcccggagcg caggccagaa gatggtagcg cgttcggcgc cgccgtgccg
      961 tgccggcggc cgtccggcgc cgcctcagtg cgccagcgcc ggctcgatcg cgtcggcggt
     1021 ggtgctgcgg cggccgccgt tgaggaagag gttgagcgtg accgcggtga tcgccgcgag
     1081 caggatgccg ctgtgcagca ggggttcgag cgcgtgcggc atctgctgga agaacttcgg
     1141 cgccaccatc gggatcatgc cggcgcccac cgacagcgcg acgatgtaga ggttgaagcg
     1201 gttgttgcgc aggtcggccg cgagcaggat cttgatgccg gtggcggcga ccatgccgaa
     1261 catcaccagg ccggcgccgc cgagcacgta ctgcggcacc gaggcgatga tcagcgccat
     1321 cttggggaag aggccgaagg ccatcaggat cgcgccgccc gccacgcaca cccagcggct
     1381 cttgacgccg gtgacgccca ccaggccgac gttctgcgag aagctggtgt gcgggaaggt
     1441 gttgaggaag ccgccgatca gggtgccgag gccatcggtg cgcagcccgc gggtgaggtc
     1501 gtcgcgcgac agctggcggc cggtgagctc gccgagcgcg aggaacatgc cgagcgactc
     1561 gaccatcacc accaccatca ccaggcacat ggtggcgatc gagatcgggt cgaagatcgg
     1621 cgcgccgaag tgcagcggcg tcacgatcga cacccagggc gcgtcggcga ggccgtcgaa
     1681 gccgaccttg ccgagcgcga tcgcgatcgc gaagcccacg cccatgccga ccagcaccgc
     1741 cacgttggac aggaagccac gaccgtagcg ggtgatcgcc agcaccacga ccagcacgat
     1801 ggcggcgatg gcgaggtatt ccggggcacc gtaggcgggg ttgtccacca tcagtccggt
     1861 cgccgcgtcg cggatgcgcg gctggccgcc ggccgaccag ttgatcgcca caggcatcag
     1921 cgagaggccg atcaccgcga tgatcgagcc ggtgaccacc ggcgggaaca gcggcagcgc
     1981 gcgcgccacc aggggcgcga acaggatgcc gaagaggccg gcggcgatgc ccgcgccgag
     2041 gatggcgttg atgccgagcg cggggttgcc cgccatcgcc accatcggcc cgaccgcggc
     2101 gaaggtgacg cccatcatga tcggcaggcg gatgccgaag cggccgacgc cgagcgcctg
     2161 gatgatggtg accaggccgc aacacagcag gtcggcgttg atgagaaagg cgatctggtc
     2221 cttgggcagc ttgagcgcgc cgccgacgat cagcggcacc gcgatcgcgc cggcgtacat
     2281 caccagcacg tgctgcaggg cgagggtgaa caggcgcccg ccgggcgggc actggtctac
     2341 cgggtcgagt tcgagcggcc ggctcgtgcg ggtttgatcg gacatcgtgg cgtctccttg
     2401 tcgttatgga ccgatgagtc cagtctagga tcgcgccgtg atatggaaac ccttatgggc
     2461 ttataggacg gatcagggga tccgtatggt gtgtgcgggg agggggaggc gtggtggggc
     2521 aggggaagga gagcgggagc ctgcggcgga agggcgaagg cggtctctcg ggcgccgcat
     2581 cgcgacttgc gtcgctccca cgaaaaccgt cgcgtcgcgg gacacaccgg atc
//
LOCUS       DAOQNN010000438         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_29674_length_2629_cov_8.721834, whole genome shotgun sequence.
ACCESSION   DAOQNN010000438 DAOQNN010000000
VERSION     DAOQNN010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29674_length_2629_cov_8.721834"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKV97_15805"
     CDS             <1..389
                     /locus_tag="PKV97_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNB07399.1"
                     /translation="SPAQSAAAAPAAAGDDFPALPGIDRERAMQRLGKDRDMFIGLLG
                     LFIEDNAGVVAATRADLARGERESAARRMHTLRSNAGFICALAIMQAAAALEKAIAQD
                     EPDVAARLDELAADIAGLVEAGRAFL"
     gene            complement(403..1581)
                     /locus_tag="PKV97_15810"
     CDS             complement(403..1581)
                     /locus_tag="PKV97_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNB07400.1"
                     /translation="MAVMPSAALAVLVVALTSLGPLSTDFYLPALPAIARALHTDSAG
                     VQLTLSVYLLGFGAGQLLVGPLSDRCGRRPVMLWGMLVFLLSTLVCVFAESLAVLVGA
                     RLVQALGACAGPVLGRAVVRDLYGPAESARVLSHVSTATALAPLLAPLFGGWLTAAWG
                     WRATFVALAIYAMVLMLAVAALLKETNRHPDAHAMRPARMLANYRTLLADPAYRGALL
                     IGCGAFAALFAFISGSPFVFIEHFGMSPQQMGLAFGLNVTGFMIGSTLSARHSRPLGP
                     ARLIRYGVWIGAACGVLLAALAFASPQHPAAVMLPMWGVTAAIGLILPNATALGLAGY
                     PKMAGAAASLMGFVQMGLGAGAGMLVGHGVQHGTAALGLTVAAGMLFALAAWSRSPAR
                     "
     gene            complement(1855..>2629)
                     /locus_tag="PKV97_15815"
     CDS             complement(1855..>2629)
                     /locus_tag="PKV97_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNB07401.1"
                     /translation="SMAYDLHGPVRPTPQAAPEPTTAARDDAWDYERAVAELERLDIP
                     ITMAQFVRALEAGDQQLCSLFLHAGFDIDGRDARQWTPLMIACFHGREALALELIRLG
                     ASVDATDADGYTPLHWASVNGYQKVGEVLVRRQAEVNATSNAGITPLLQAAARGHLGV
                     VRLLLDRKAKVNLVAADGSTALLKAVANGHWEIVNTLLDAGASTQATMKNGTTLVDIA
                     ARSKDERIRERIAIAARMEARGDAPIQRDEPPPLTGTIY"
BASE COUNT          396 a          909 c         1006 g          318 t
ORIGIN      
        1 cgtcgccggc gcagagcgca gcagcggcgc ctgccgcggc tggcgacgat ttccccgcgc
       61 tgcccggcat cgaccgcgag cgtgcgatgc agcgcctcgg caaggatcgc gacatgttca
      121 tcggcctgct cgggctcttc atcgaggaca acgccggggt ggtggcggcg acccgcgccg
      181 atctcgcacg cggcgagcgc gaatcggcgg cgcgccgcat gcacacgctg cgcagcaacg
      241 ccggcttcat ctgcgcactc gcgatcatgc aggcggccgc agcgctggag aaggcaatcg
      301 cgcaggacga gcccgacgtg gcggcgcgcc tggacgaact cgcggcggac atcgccgggc
      361 tggtggaggc cggccgtgcg ttcttgtgaa cgccgcccgc gttcagcgcg cgggtgagcg
      421 gctccatgcg gcgagggcga acagcatgcc ggccgcgacg gtgagaccga gcgccgcggt
      481 gccgtgctgc acgccgtgcc cgaccagcat gcccgccccg gcgcccaggc ccatctgcac
      541 gaaacccatc agcgaggccg cggcaccggc catcttggga tagccggcga gtccgagcgc
      601 ggtggcgttg ggcaggatca gcccgatcgc cgcggtgacg ccccacatcg gcagcatcac
      661 cgccgccggg tgctgcgggc tggcgaaggc gagcgcggcg agcagcacgc cgcacgccgc
      721 gccgatccac acgccgtagc ggatcaggcg cgccgggccg agggggcgcg agtgccgcgc
      781 cgacagcgtc gagccgatca tgaagccggt gacgttgagc ccgaaggcca ggcccatctg
      841 ctgcggcgac atgccgaagt gctcgatgaa cacgaagggc gagccggaga tgaaggcgaa
      901 cagcgcggcg aaggcgccgc agccgatcag cagcgcgccg cggtaggcgg ggtcggcgag
      961 cagcgtacgg tagttggcca gcatccgggc cgggcgcatc gcatgggcat cggggtggcg
     1021 gttggtctcc ttcagcagcg cggccacggc gagcatcagg accatggcgt agatcgcgag
     1081 cgcgacgaag gtcgcgcgcc agccccaggc ggcggtgagc cagccgccga acagcggcgc
     1141 gagcagcggt gcgagcgcgg tggcggtgga cacgtgcgag agtacgcgcg ccgattcggc
     1201 ggggccgtag aggtcgcgca ccacggcacg gccgagtacc ggccctgcgc aggcgccgag
     1261 ggcctgcacc aggcgcgcgc ccaccagcac ggcgagcgat tccgcgaaca cgcacaccag
     1321 cgtggagagc aggaacacca gcatgcccca cagcatcacc ggccgccgtc cgcagcgatc
     1381 cgagagcggg ccgacgagca actgcccggc gccgaagccg agcaggtaga ccgacagggt
     1441 gagctgcacg ccggcgctgt cggtgtgcag ggcacgcgcg atcgcgggca gggcgggcag
     1501 gtagaagtcg gtggagagcg gcccgagcga ggtcagcgcc accaccagca ccgccagcgc
     1561 tgcggagggc atcaccgcca tgggccgccc gcatccgtcc gcagcgaagg gagcagggcc
     1621 ggtggcgcgg gcggggtgga gtgtttcgtc atcggcaatg aggcggtcca gcggagcgtg
     1681 attgtatccc gcctccgcgc cgtcgccgcc gttcatccac cgcagcgatc gcgactcgcg
     1741 tcgctcccac gatcaaccgg cacggtatcc gcagcgcccc ggcgcgacgc gagtcgcgag
     1801 cgggcggacg gatcgcacgg acggtcccgg caaggcggcg ttcgcgcccc gccgtcagta
     1861 gatcgtgccg gtgagcggtg gcggctcgtc gcgctggatc ggcgcatcgc cgcgcgcctc
     1921 catgcgcgcg gcgatggcga tgcgttcgcg gatgcgctcg tccttcgaac gtgcggcgat
     1981 gtcgaccagg gtggtgccgt tcttcatcgt cgcctgcgtg gaggcgccgg cgtcgagcag
     2041 cgtgttgacg atctcccagt gtccgttggc gaccgccttg agcagtgccg tggagccgtc
     2101 cgcggcgacg agattgacct tggccttgcg gtcgagcagc aggcgcacga cgcccaggtg
     2161 gccgcgcgcc gcggcctgca gcagcggcgt gatgccggcg ttgctcgtgg cattgacctc
     2221 ggcctggcgg cgcacgagca cctcgccgac cttctggtag ccgttcaccg aggcccagtg
     2281 cagcggcgtg tagccgtcgg cgtccgtcgc atccacgctg gcgccgagcc ggatcagctc
     2341 gagggcgagc gcctcgcggc catggaagca ggcgatcatc agcggcgtcc actggcgcgc
     2401 gtcgcggccg tcgatgtcga agccggcatg caggaagagc gagcacagtt gctggtcgcc
     2461 agcctccagc gcgcgcacga actgcgccat cgtgatcgga atgtcgaggc gctcgagctc
     2521 ggcgaccgcg cgctcgtagt cccaggcgtc gtcgcgcgcg gccgtcgtgg gctcgggggc
     2581 cgcttgcggg gtgggacgca ccgggccgtg gaggtcgtag gccatgctc
//
LOCUS       DAOQNN010000439         2617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_29904_length_2617_cov_7.433255, whole genome shotgun sequence.
ACCESSION   DAOQNN010000439 DAOQNN010000000
VERSION     DAOQNN010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29904_length_2617_cov_7.433255"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PKV97_15820"
     CDS             complement(<1..583)
                     /locus_tag="PKV97_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HNB07402.1"
                     /translation="MILYLHGFRSAPASIKARALKARMVERGLAERFWCAQLPVSPRA
                     AIALAETEIARIRAAGLEPTVVGSSLGGYYATWLAERHDLRAVLINPAVVAPLSLEAY
                     VGVQDNLYTGERFEFTRGHIDELRAIEVPTITRPERFWLLVETGDEVLDYRHAVDKYA
                     GARQTVLEGGDHGFSRWNDYLDEVLAYAGLGDTR"
     gene            complement(597..1421)
                     /locus_tag="PKV97_15825"
     CDS             complement(597..1421)
                     /locus_tag="PKV97_15825"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNB07403.1"
                     /translation="MDLSLLLVALVLGIVEGLTEFLPVSSTGHLIILGDLLGYNDDTS
                     KVFKIVIQLAAILAVCWDYRERLIKVASGITTEPSAQRFVWLLFIGFLPAAVLGLMFH
                     STIKGLLFNPITVATALVVGGLIILYVEKRAYHPRLNSVDELRWTDALKVGFAQALAM
                     IPGTSRSGATIMGGVIFGLSRKTAAEFSFFLAIPTMFAATAYDLYKNWALLRVEDLPV
                     FAVGFIASFVAAMWAVKSFIKFISNHTFVAFAWYRIVFGIFVLATWYFGWVEWSEP"
     gene            complement(1512..1766)
                     /locus_tag="PKV97_15830"
     CDS             complement(1512..1766)
                     /locus_tag="PKV97_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNB07404.1"
                     /translation="MDCRPGCAACCIAPSISSPIPGMPDGKPAGVRCVQLDEAGHCRL
                     FGDPRRPAVCASLRPGAEMCGTDREHALLFLATLEAMTAT"
     gene            complement(1770..2285)
                     /locus_tag="PKV97_15835"
     CDS             complement(1770..2285)
                     /locus_tag="PKV97_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2478 domain-containing protein"
                     /protein_id="HNB07405.1"
                     /translation="MPAHAIAAVVYKPTDNIEALLARAARALGERGVRLGGVLQHDIA
                     TVIEDPCAMQLENLETGESIPLSQELGRGSISCRVDPDALAQGSMAVRGAIERGVELV
                     IINKFGAQEVSGAGLRDEMAETVVAGVPLLTAVGERFLPEWKEFTGGEGVLLEPTLDA
                     ILAWWAAPSAG"
     gene            2399..>2617
                     /locus_tag="PKV97_15840"
     CDS             2399..>2617
                     /locus_tag="PKV97_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07406.1"
                     /translation="MRAEHKGARRPSALPNFDDCLSGDRPRLRRMARDLSHPSRADER
                     ATAQRLRLRADFDAENAVPVGEPPKVAPL"
BASE COUNT          434 a          848 c          967 g          368 t
ORIGIN      
        1 ggcgggtatc gcccagcccc gcataggcga gcacctcgtc caggtagtcg ttccagcgcg
       61 agaagccatg gtcgccgcct tcgagcacgg tctggcgggc accggcgtac ttgtccaccg
      121 cgtggcggta gtcgagcacc tcgtcgccgg tctccaccag cagccagaag cgctccggcc
      181 gggtgatcgt cggcacctcg atcgcgcgca gttcgtcgat gtggccgcgg gtgaactcga
      241 aacgctcgcc ggtgtagagg ttgtcctgca cgccgacata ggcctcgagc gagagcggcg
      301 cgaccacggc cggattgatc agcaccgcgc gcaagtcgtg gcgctcggcg agccaggtgg
      361 cgtagtagcc accgagcgag ctgccgacca cggtgggctc gaggccggcg gcgcggatgc
      421 gcgcgatctc cgtttcggcg agcgcgatcg cggcgcgcgg cgacaccggc agctgcgcgc
      481 accagaagcg ttcggccagg ccgcgctcga ccatgcgtgc cttcagggcg cgcgccttga
      541 tcgacgccgg ggcggaacgg aagccgtgca ggtagaggat catcgtgctg cctcgatcat
      601 ggctcggacc actcgaccca gccgaagtac caggtggcga gcacgaagat gccgaagacg
      661 atgcggtacc aggcgaaggc gacgaaggtg tggttggaga tgaacttgat gaagctcttc
      721 accgcccaca tcgcggcgac gaaggaggcg atgaagccga ccgcgaacac cggcaggtct
      781 tccacccgca gcagcgccca gttcttgtac aggtcgtagg cggtggcggc gaacatggtc
      841 gggatcgcca ggaagaagga gaactccgcc gccgtcttgc gcgacaggcc gaagatgacg
      901 ccgcccatga tggtcgcgcc cgagcgcgaa gtgcccggga tcatcgccag cgcctgggcg
      961 aagcccacct tgagcgcgtc ggtccagcgc agttcatcca ccgaattcag gcgcgggtgg
     1021 taggcgcgct tttccacgta gaggatgatc aggccgccga ccaccagcgc ggtggccacc
     1081 gtgatcgggt tgaacagcag gcccttgatg gtggagtgga acatcagccc gagcaccgcc
     1141 gcgggcagga agccgatgaa cagcagccag acgaagcgct gcgcgctcgg ttcggtggtg
     1201 attccgctcg cgaccttgat caggcgctcg cgatagtccc agcacacggc gaggatggcg
     1261 gcgagctgga tgacgatctt gaacaccttg ctggtgtcgt cgttgtagcc gagcaggtcg
     1321 cccaggatga tgaggtggcc ggtggaggac accgggagga attcggtcag gccttcgacg
     1381 atgccgagga cgagcgcgac gaggaggagg gagaggtcca tgcggccggg ctggaagtgg
     1441 gggaagggtg gagggatcgg aggccggatt atagccggcg ggatgttgca ctgcggggta
     1501 ggtttgcccg ctcaggtcgc cgtcatcgct tcgagggtgg cgagaaaaag cagggcatgc
     1561 tcgcggtcgg tgccgcacat ctcggcgccg gggcgcaggc tggcgcacac cgccggccga
     1621 cgcgggtcgc cgaacaggcg gcagtgcccg gcctcgtcga gctgcacgca gcgcacaccc
     1681 gcgggtttgc cgtccggcat gccggggatg ggcgaggaga tcgagggcgc gatgcagcag
     1741 gcggcgcagc cggggcggca gtccatgcct cagccggcgc tcggcgccgc ccaccaggcg
     1801 aggatggcgt cgagcgtggg ctcgagcagc acgccctcgc caccggtgaa ctccttccac
     1861 tcggggagga agcgctcgcc taccgccgtg agcaggggta cgccggccac gaccgtctcg
     1921 gccatttcgt cgcgcaggcc ggcgcccgac acttcctgcg cgccgaactt gttgatgatg
     1981 accagctcga cgccgcgctc gatcgcgccg cgcaccgcca tcgagccttg cgcgagcgcg
     2041 tccgggtcga cccggcagga gatcgagccg cggccgagct cctgtgacag cgggatgctc
     2101 tcgccggtct ccaggttctc gagctgcatc gcgcaggggt cctcgatcac cgtggcgatg
     2161 tcgtgctgca gcacgccgcc caggcgcacg ccacgctcgc ccagggcgcg cgcggcgcgg
     2221 gcgagcaggg cctcgatatt gtcggtgggc ttgtagacga cggcggcgat ggcgtgtgcg
     2281 ggcatggtcg ggctcggcgg gcggctcgtt cgtggatggg cctcattgtc gcactgcggc
     2341 gccaacaagg cgaagatgcg ggagataatg cgcggctcga cgaagccgaa acggatccat
     2401 gagggctgaa cacaagggcg cgcgccgccc gtccgcgctg ccgaacttcg atgactgtct
     2461 gagcggcgac cgcccgcgcc tgcgccgcat ggcgcgcgac ctctcccacc cgtcacgcgc
     2521 ggacgagcgc gccacggcgc agcgcctgcg cctgcgggcg gacttcgatg cggagaatgc
     2581 cgtacccgtg ggggaacccc ccaaggttgc gcccctg
//
LOCUS       DAOQNN010000440         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_29986_length_2612_cov_7.015252, whole genome shotgun sequence.
ACCESSION   DAOQNN010000440 DAOQNN010000000
VERSION     DAOQNN010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29986_length_2612_cov_7.015252"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PKV97_15845"
     CDS             <1..1024
                     /locus_tag="PKV97_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB07407.1"
                     /translation="LYVEDLRCVFDVSRPWLERVLAREPRRWLRAVDGVSFAIGRGCT
                     FALVGESGCGKSTVARLIVGLHEPSAGRIVFDGRDVAEVRGSAERQQLRSRFQMIFQD
                     PYASLNPRWRVERIIAEPIRVFGLEKDDAAIRARVAGLLDQVGLSPADGAKYPHEFSG
                     GQRQRICIARALANRPEFLVCDEPTSALDVSVQAQILNLMRDLQDELGLTFLFISHDL
                     AVVRHMADEVGVMYLGRIVEIAASRALFERPRHPYTRMLLAAIPDFSRRGRGDDAVRG
                     EVPNPITPPSGCSFHPRCPLADARCRGEAPVLRELDDGRRVACHAVEEGRDTIEAGAE
                     ICTEVS"
     gene            1021..1764
                     /locus_tag="PKV97_15850"
     CDS             1021..1764
                     /locus_tag="PKV97_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB07408.1"
                     /translation="MSGGTSEAARAEVRMEEAGGRTLAVVVGGGGGVGAAVVAALREA
                     GGHDEVLALGRRSSPALDLLEEASIAAAAAAVRARGVPTLVFDATGFLHGAGMEPEKS
                     WRELDAAHLAHAFAINAIGPALLMKHFLPLLPRDRRAVFATLSARVGSIGDNRLGGWY
                     AYRASKAALNQLVRTAAVELRRSRPQAICVALHPGTVDTGLSAPFAKSGLQVQAPAEA
                     AARLLAVIEGLGPGDSGEFFDHRGEPVPW"
     gene            1800..2447
                     /locus_tag="PKV97_15855"
     CDS             1800..2447
                     /locus_tag="PKV97_15855"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB07409.1"
                     /translation="MNAQEQEHMWSTRYRSAGEDYVFGTTPNRFLAAQAALLAAGASA
                     LSVADGEGRNAVWLAEQGLAVTATEISPVALEKARKLAAGRHVEVDFVLADAVEWDYP
                     EAAFDFVVAIFIQFADPAQRARIFAGMARALKPGGHLILQGYTPQQLEYRTGGPSAVE
                     NLYTAALLREAFAGLDIVHVQEYEDVLEEGVGHKGRSALVGLVARRPPALGEGRR"
BASE COUNT          328 a          940 c          973 g          371 t
ORIGIN      
        1 gctgtacgtc gaggacctgc gctgcgtctt cgacgtctcc cggccctggc tcgagcgggt
       61 gctcgcgcgc gagccgcggc gctggctgcg cgcggtcgat ggggtctcgt tcgcgatcgg
      121 gcgcggatgc accttcgcgc tggtgggaga gtcgggctgc ggcaaatcca cggtcgcgcg
      181 cctgatcgtc ggcctccatg agccctcggc cggccgcatc gtcttcgacg gccgcgacgt
      241 ggccgaggtg cgcgggagcg ccgagcgcca gcagctgcgc agccgcttcc agatgatctt
      301 ccaggacccc tacgccagcc tcaacccgcg ctggcgcgtg gagcgcatca tcgccgagcc
      361 gatccgcgtg ttcggcctgg agaaggacga cgcggcgatc cgcgcgcgcg tggccgggct
      421 gctggatcag gtcggcctgt cgccggccga tggcgcgaag tacccgcacg agttctccgg
      481 cggacagcgc cagcgcatct gcatcgcgcg cgcgctggcc aaccggcccg agttcctcgt
      541 ctgcgacgag ccgacctcgg cgctcgacgt gtcggtgcag gcgcagatcc tcaacctgat
      601 gcgcgacctg caggacgaac tcgggctcac cttcctgttc atcagccacg acctcgccgt
      661 ggtgcgccac atggccgacg aggtgggcgt gatgtacctc ggccgcatcg tcgagatcgc
      721 tgccagtcgc gcactgttcg agcgcccgcg ccacccctac acgcgcatgc tgctggcggc
      781 catccccgac ttttcccgcc gtggtcgggg cgacgatgcg gtgcgtggcg aggtccccaa
      841 cccgatcacc ccgccatccg gctgcagctt ccacccgcgc tgcccgctcg cggacgcgcg
      901 ctgccggggc gaggcgccgg tcctgcgcga gctcgacgac ggccggcgcg tggcctgtca
      961 tgcggtggag gaggggcgcg acacgatcga ggcgggcgcg gagatctgca cggaggtgtc
     1021 atgagcggcg gtacgagcga ggcggcgcgc gccgaggtgc gcatggagga agccggcggc
     1081 aggacgctcg ccgtggtcgt gggcggcggc ggtggggtgg gcgcggccgt ggtcgccgcg
     1141 ttgcgcgagg cgggtggcca cgacgaggtc cttgccctcg ggcgccggag cagccccgcg
     1201 ctcgatctgc tcgaagaggc ctcgatcgcc gctgcggccg ccgcggtgcg tgcgcgcggc
     1261 gtgccgaccc tggtcttcga cgccaccggt ttcctgcacg gcgcgggcat ggagccggag
     1321 aagagctggc gcgagctcga tgcggcccac ctggcgcacg cgttcgcgat caacgcgatc
     1381 ggcccggccc tgctgatgaa gcatttcctg ccgctgctgc cgcgcgaccg ccgcgcggtg
     1441 ttcgccacgc tgtcggcgcg cgtgggcagc atcggtgaca atcggctcgg cggctggtac
     1501 gcctaccgcg cctcgaaggc ggcgctcaac cagctggtgc gcaccgccgc ggtcgagctg
     1561 cgccgcagcc ggccgcaggc gatctgcgtg gcgctgcatc cgggcacggt cgacaccggg
     1621 ctgtcggcgc ccttcgccaa gtccgggctg caggtgcagg cccccgccga ggcggcggcg
     1681 cgcctgctcg cggtgatcga gggcctcggg ccgggcgact cgggcgaatt cttcgaccat
     1741 cgcggcgaac ccgtgccgtg gtgaccgccg cgccgccgcg gcgctttcag ggaccaggga
     1801 tgaacgcaca ggaacaggaa cacatgtggt ccacccgcta tcgcagcgcg ggcgaggact
     1861 acgtcttcgg caccaccccc aaccgcttcc tcgccgcaca ggcggcgctg ctcgcagccg
     1921 gcgccagcgc gctctcggtg gccgacggcg aggggcgcaa cgcggtgtgg ctggccgagc
     1981 aggggctcgc ggtgaccgcc accgagatct ccccggtggc gctggagaag gcgcgcaagc
     2041 ttgccgccgg ccgccatgtc gaggtcgact tcgtgctcgc cgacgcggtc gagtgggact
     2101 acccggaggc cgccttcgac ttcgtggtgg cgatcttcat ccagttcgcc gacccggcgc
     2161 agcgtgcgcg catctttgcc ggcatggcgc gcgcgttgaa gccgggcggc cacctgatcc
     2221 tgcagggcta cacgccgcaa cagctcgaat accgcaccgg cggcccgtcc gcggtggaga
     2281 acctgtatac ggccgcactg ctgcgcgagg ccttcgccgg gctcgacatc gtgcatgtgc
     2341 aggaatacga ggacgtgctg gaggagggtg tgggccacaa ggggcgttcg gccctggtcg
     2401 gcctggtcgc gcgccgtccg cccgcgctgg gggaggggcg ccgatgaacg cgatgcgcgc
     2461 cgtactcacc ggacacaacc gcggcctcgg cgaggcggtc gccgcggagc tgctcgatcg
     2521 cggcatcgcg gtgctcgcgc tcgcacgccg cggcaatccc gcgctcgccg cccgccacgg
     2581 cgagcgcctg cgcgaggtcg cgctcgatct cg
//
LOCUS       DAOQNN010000441         2604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_30162_length_2604_cov_10.307572, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000441 DAOQNN010000000
VERSION     DAOQNN010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30162_length_2604_cov_10.307572"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(444..2519)
                     /locus_tag="PKV97_15860"
     CDS             complement(444..2519)
                     /locus_tag="PKV97_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HNB07410.1"
                     /translation="MFKKILIANRGEIACRVIKTARKMGIKTVAVYSEADKDARHVEL
                     ADEAVLLGPAPSRESYLVADKIIAACKSTGAEAVHPGYGFLSENAAFAKRCEEEGIAF
                     IGPRYFSIEAMGDKIASKKLANEAKVNTIPGYNDAIDTPEQAVEIAKGIGYPVMIKAS
                     AGGGGKGLRVAFNDKEAFEGFSSCRNEARNSFGDDRVFIEKYVLEPRHIEIQVLGDSH
                     GNVVYLNERECSIQRRHQKVIEEAPSPFISDATRKAMGEQAVALAKAVKYQSAGTVEF
                     VVGKDQDFYFLEMNTRLQVEHPVTECITGLDLVEQMIRVAAGEKLSFGQADIKREGWA
                     IECRINAEDPFRNFLPSTGRLVKYAPPAADLEQGTETLQGPAYNGGKFGGVRVDTGVY
                     EGGEIPMFYDSMIAKLIVHGRDRNEAIAKMREALNAFVIRGISSNIPFQAALLAHPKF
                     VTGQFNTGFIAEHYSKGFKAEDVPHDDPAFLVALAAFVRRKARERAAGISGQLPGHEV
                     AHKNEMVVVQLGAEGQHVYHDVTLGDFDPAAGTVDVGVGGKTFAIQSHTKLGDVLMTG
                     LVNGRPFSAQVEQGSAKRPLAIKVAHNGTAIEALVLLPRAAELFKLMPYKAPPDLSKF
                     LLSPMPGLLVDVAVTAGQKVLAGEKLAVIEAMKMENILVATQDCVVKEVRAAKGESLA
                     VDQAIIAFE"
BASE COUNT          392 a          841 c          868 g          503 t
ORIGIN      
        1 gccgatggcg atctgctgga tgccgaggat cttgaagggt ttggtcatgg tgcggttgaa
       61 gacaatgaaa gtttgggtcg gtagaccacc ccgggcaggc cttgcagcac tggcgcgcag
      121 acgcatgaac ccgacgcgcc ttgcctacac gttcccatct ggttctgagg ggatatgggt
      181 gacatccact ccggcgatag gcttcaagaa gccacgtgca tctctgatgt cccgtagggg
      241 gacgatccat gcacgcccgg cgccatcggc gtggatgtca atgcgctgcc caggactcaa
      301 tcgcagtgct tcgcgcacgt ctttgggcag aacgatccga aacctagagg aaatggcaac
      361 gctcgtcaca ggcagtccct tcgcaacatg gccccatcat ggccgtgctg agattcagcg
      421 acaagcaaag cgctgccggc ccctcactcg aacgcgatga tggcctgatc cacggccagg
      481 ctctcgccct tggccgcacg cacttccttg accacgcagt cctgcgtggc gacgaggatg
      541 ttctccatct tcatcgcctc gatgaccgcg agcttctcgc cggcgagcac cttctggccc
      601 gccgtcacgg cgacgtcgac cagcaggccg ggcatcggcg agagcaggaa cttggacaga
      661 tcgggcggtg ccttgtaggg catcagcttg aacagctcgg ccgcacgcgg cagcaacacc
      721 agcgcttcga tcgcggtgcc gttgtgcgcc accttgatcg ccagcgggcg cttggccgag
      781 ccctgctcga cctgggcgct gaaggggcgg ccgttgacca ggccggtcat cagcacgtcg
      841 ccgagcttgg tgtggctctg gattgcgaag gtcttgccac cgacgccgac gtccaccgtg
      901 ccggccgccg ggtcgaagtc gcccagcgtg acgtcgtggt agacgtgctg gccttcggcg
      961 ccgagctgca ccacgaccat ctcgttcttg tgcgccacct cgtggccggg cagctggccg
     1021 ctgatgccgg cggcacgctc acgcgccttg cggcgcacga aggcggccag cgcgaccagg
     1081 aaggctgggt cgtcgtgcgg cacgtcctcg gccttgaagc ccttgctgta gtgctcggcg
     1141 atgaagccgg tgttgaactg gccggtgacg aacttcgggt gcgccagcag cgcggcctgg
     1201 aaggggatgt tgctgctgat gccgcggatc acgaaggcat tcagcgcctc gcgcatcttg
     1261 gcaatcgcct cgttgcggtc ccggccgtgc acgatgagct tggcgatcat cgagtcgtag
     1321 aacatcggga tctcgccacc ctcgtagacg ccggtgtcca cgcgcacgcc gccgaacttg
     1381 ccgccgttgt aggcgggacc ctgcagcgtt tcggtgccct gctccaggtc agcggccggt
     1441 ggggcgtact tcaccaggcg gccggtggag ggcaggaagt tgcggaaggg gtcttccgcg
     1501 ttgatgcggc actcgatggc ccagccttcg cgcttgatgt cggcctggcc gaagctcagc
     1561 ttctcgcccg cggccacgcg gatcatctgc tcgaccaggt ccaggccggt gatgcactcg
     1621 gtgaccgggt gctccacctg caggcgggtg ttcatttcca ggaagtagaa gtcctggtcc
     1681 ttgccgacca cgaattcgac cgtgccggcg gactggtact tcaccgcctt ggccagcgcc
     1741 acggcctgct cgcccatggc cttgcgcgtg gcgtcggaga tgaaggggct cggcgcctct
     1801 tcgatcactt tctggtggcg gcgctggatc gagcactcgc gctcgttcag gtagaccacg
     1861 ttgccgtggc tgtcgccgag cacctggatc tcgatgtggc gcggctcgag cacgtacttc
     1921 tcgatgaaga cgcggtcgtc gccgaagctg ttgcgcgcct cgttgcggca ggacgagaag
     1981 ccctcgaagg cttccttgtc gttgaaggcc acgcgcaggc ccttgccgcc gccgccggcc
     2041 gaggccttga tcatcaccgg gtagccgatg cccttcgcga tctccacggc ctgctcgggc
     2101 gtgtcgatgg cgtcgttgta gccggggatg gtgttgacct tggcctcgtt ggccagcttc
     2161 ttcgaggcga tcttgtcgcc catcgcctcg atgctgaagt agcgcgggcc gatgaaggcg
     2221 atgccctctt cctcgcagcg cttggcaaag gcggcgttct ccgacaggaa gccgtagccc
     2281 gggtgcaccg cttcggcgcc ggtgctcttg caggcggcga tgatcttgtc ggccaccagg
     2341 taggactcgc gcgagggcgc cgggcccagc agcacggcct cgtcggccag ctccacgtgg
     2401 cgggcgtcct tgtcggcctc ggagtagacg gcgaccgtct tgatgcccat cttccgggcg
     2461 gtcttgatga cgcggcaggc gatttcgccg cggttcgcga tcaggatctt cttgaacatg
     2521 ttcgtgtgct ccgcggggct tacagaggaa tgttcccgtg cttgcgccac gggttctcga
     2581 tcttcttgtc gcgcagcatc gtca
//
LOCUS       DAOQNN010000442         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_30301_length_2597_cov_6.472069, whole genome shotgun sequence.
ACCESSION   DAOQNN010000442 DAOQNN010000000
VERSION     DAOQNN010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30301_length_2597_cov_6.472069"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..562
                     /locus_tag="PKV97_15865"
     CDS             11..562
                     /locus_tag="PKV97_15865"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNB07411.1"
                     /translation="MGNRLSKIYTRTGDAGTTGLGDGKRVSKNSLRIHALGEVDETNA
                     IIGLLLCEELPEDVRVLLTNVQHDLFDLGGEVCIPGMSMITGKQVTHLEEELDRFNEP
                     LEPLKDFILPGGTRAAAYAHHARTVCRRAERALVALALEESVNDAPRQYLNRLSDLLF
                     VLGRVLNRAGGRGDVLWQKRKNA"
     gene            complement(722..>2597)
                     /locus_tag="PKV97_15870"
     CDS             complement(722..>2597)
                     /locus_tag="PKV97_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB07412.1"
                     /translation="IGALYRALWEHAAGARQLLLYAFLLLLASQLFKLAVPWLAGNAI
                     NQIQAGGLEGMSEAGRWLGMVFAAIVASWLLHGPGRILERNVALKVRERLAALLVGRL
                     LAAPLAWHERHHSGETTHRMQQSTHALYDFAQSQFIYLQNTVRLVGPIVALWLISPLV
                     GAVALVGYGLIGLLITRFDRVMMRVAVEENRAERAYSATLVDVLGNVLSVLALRRGEG
                     VARLIGERLAAVFGPLKKSIVLNEAKWCAVDLLASALWCVLVGVYAAEAAGVFGFGAA
                     AGGAAAAAGTIALGKVFMVYEYSQQAGGVITAIAAHFQSLARQRADYAAAEPLLSAAP
                     APRPADGEDIAASGWRTLSLEGVCFAHGDGRASALELDRLALVRGRRYALVGPSGAGK
                     TTLLRLLAGLDAPTAGVLSIDGEAVPAAATAAVVGERVPSAGGLPSDAGGLAAMPARL
                     RHLATLIPQQAEMFDGTLAENLLLGAGRTPERIEAALHAACADSVVEGLAQGLDTRVV
                     EGGANWSGGQRQRLALARGVLAAAGTSLLLLDEPTSSLDPETEARVYQRLFAHFPDAA
                     LVSSVHRLHLLDRFDEVILMRAGRIEAVGRAWELAQSSALFRELLAAQAVSGEEASQG
                     "
BASE COUNT          398 a          989 c          898 g          312 t
ORIGIN      
        1 cgaggacatc atgggtaacc gcctctccaa gatctacacc cgcaccggcg acgccggcac
       61 caccggcctg ggcgacggca agcgcgtctc caagaacagc ctgcgcatcc acgccctggg
      121 cgaggtcgac gagaccaacg ccatcatcgg cctgctgctg tgcgaggagc tgcccgagga
      181 cgtgcgcgtg ctgctgacca acgtgcagca cgacctcttc gacctcggcg gcgaggtgtg
      241 catcccgggc atgagcatga tcaccggcaa gcaggtgaca caccttgaag aagagctcga
      301 ccgcttcaac gaaccgctcg agccgctcaa ggacttcatc ctgcccggcg gcacccgcgc
      361 cgccgcctac gcccaccacg cccgcacggt gtgccgccgc gccgagcgcg cgctggtggc
      421 gctggcactc gaggagtctg tgaatgacgc cccgcgccag tacctcaacc gcctctccga
      481 cctcctcttc gtgctcggcc gcgtgctcaa ccgcgccggc ggccgcggcg acgtgctttg
      541 gcagaagcgg aagaatgcct gagggccgcc tcccgcagcc acgtcgtttc tcgtaggagc
      601 gacgcgagtc gcgattgcgg cgcttggaat ccgcgcctcc gccccggaag cgcccgatcg
      661 cgacgggcgt cgctcatgcc ggcgcgcccg gacggggtgc agcgcgtgcc gctccccggc
      721 ttcacccctg cgacgcctcc tctccgctca ccgcctgggc ggcgaggagt tcgcggaaca
      781 aggccgagct ctgcgccagc tcccaggcgc ggccgaccgc ctcgatgcgg ccggcgcgca
      841 tcaggatgac ctcgtcgaaa cgatcgagca ggtgcaggcg gtgcaccgag gacaccagcg
      901 ccgcgtccgg gaaatgcgcg aacaggcgct ggtagacgcg cgcctcggtc tccgggtcca
      961 ggctgctggt gggttcgtcg agcagcagca ggctggtgcc tgcggcggcg agcacgccgc
     1021 gggcgagcgc gaggcgctgg cgctgcccgc cggaccagtt cgcgcccccc tccaccaccc
     1081 gcgtgtccag cccctgcgcc aggccctcga ccacggagtc tgcgcacgcc gcatgcagcg
     1141 cggcctcgat gcgctcgggc gtgcgaccgg caccgagcag caggttctcg gccagcgtgc
     1201 cgtcgaacat ctcggcctgc tgcgggatca gggtggcgag atggcgcaga cgcgccggca
     1261 tcgccgccag ccccccggcg tcgctcggca agccgccagc gctgggaacg cgctcgccga
     1321 cgaccgccgc ggtggcggct gcgggcaccg cctcaccgtc gatcgacagg acacccgccg
     1381 tgggcgcgtc cagcccggcg agcaggcgca gcagggtggt cttgcccgcg ccgctcgggc
     1441 cgaccagggc atagcggcgg ccgcgcacga gcgccaggcg gtcgagctcg agcgcgctcg
     1501 cgcgcccgtc gccatgggcg aagcacacgc cctccaggct cagcgtgcgc cagcccgagg
     1561 cggcgatgtc ctcgccgtcg gccggacgcg gcgccggcgc ggcggacaac agcggctcgg
     1621 ctgcggcgta gtcggcacgc tggcgcgcga gcgactggaa gtgcgccgcg atcgcggtga
     1681 tcaccccgcc ggcctgctgc gagtactcgt acaccatgaa gaccttgccg agcgcgatcg
     1741 tgccggccgc cgccgcagcc ccgcccgcgg ccgcgccgaa gccgaacaca cccgcggcct
     1801 cggccgcata caccccgacc agcacgcacc acagcgcgct cgcgagcaga tccaccgcgc
     1861 accacttggc ctcgttgagc acgatgctct tcttcagcgg cccgaacacc gccgccaggc
     1921 gctcgccgat cagccgcgcc acgccctcgc cgcggcgcag cgcgagcacc gacagcacgt
     1981 tgccgagcac atcgaccagg gtcgcggagt aggcgcgctc ggcgcggttc tcctccaccg
     2041 ccacgcgcat catcacgcgg tcgaagcggg tgatgagcag gccgatgagg ccgtagccga
     2101 ccagggccac cgcacccacc agcggcgaga tcagccacag cgccacgatc ggccccacca
     2161 ggcgcacggt gttctgcagg tagatgaact ggctctgcgc gaagtcgtag agcgcatgcg
     2221 tgctctgctg catgcgatgg gtggtctcgc ccgagtggtg gcgctcgtgc caggccagcg
     2281 gcgcggcgag caggcggccc acgagcaggg cggcgaggcg ctcacgcacc ttgagcgcga
     2341 cgttgcgttc gaggatgcgc cccggtccgt gcagcagcca gctcgcgacg atggccgcga
     2401 agaccatgcc gagccagcgc ccggcctcgc tcatgccctc cagcccaccg gcctggatct
     2461 ggttgatcgc gttgccggcc agccagggca cggcgagctt gaagagctgg gacgcgagca
     2521 gcagcaggaa ggcgtagaga agcaactggc gcgcgcctgc ggcgtgctcc cacagcgcgc
     2581 ggtagagcgc gccgatg
//
LOCUS       DAOQNN010000443         2590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_30452_length_2590_cov_8.777515, whole genome shotgun sequence.
ACCESSION   DAOQNN010000443 DAOQNN010000000
VERSION     DAOQNN010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30452_length_2590_cov_8.777515"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PKV97_15875"
     CDS             <1..1153
                     /locus_tag="PKV97_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNB07413.1"
                     /translation="DIQPFHVMELLRRARELEAQGRDIIHMEVGEPDFGTPAPVVEAA
                     TRFLAGGDVHYTPALGLPALREAIARFYHDRFGADVAPERIVVTAGASGALMLALAAT
                     TDPGDEWLLPDPGYPSNRHLIRAFEGVARALAVDAASRYQPRPEQVDAAWGERTRGLM
                     VATPSNPTGTLLSVAEIAALHRRTRARGGVLLVDEIYQGLTYGVESASALADPVLNAA
                     DDVFVVNSFSKYFGMTGWRLGWLVAPAGFVRELEKLAQHFFISPSTPAQHAALAAFAP
                     ATIAILEERRHEFAQRRDVLLPALRALGFGIAAEPQGAFYVYADVSALADDSEALARR
                     MIEEAGVAATPGLDFGHHLPRRHLRIAYTTHGARLLEAAERIRRLGLGS"
     gene            complement(1467..2381)
                     /locus_tag="PKV97_15880"
     CDS             complement(1467..2381)
                     /locus_tag="PKV97_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNB07414.1"
                     /translation="MKHATRFKALARQAGGAARSLAHGAVLSVGLMGMGWLGTQGAGA
                     ADVRYGDVLGLQAEPTASELAMASVLSFAPRRGEMGASAPSFAASIPSLAASTRLHES
                     LPVAAPGLIERKAARAVAEPIPPVFHQPALRDIAALVDNPFEDEGALSDEMERVRDWI
                     ADTYQVSEETIEPALAAAETHAEELGFDPLLIVAIMAVESSFNHRAVSNMGALGLMQV
                     IPRYHKDKIGPNRGRNVLFDPEVNVRVGTLVLHEGLQRYGSMQRALQYYNGALNDPKA
                     RYTRKVMALKKRLMLIAGRDRSSAEQAS"
BASE COUNT          367 a          948 c          907 g          368 t
ORIGIN      
        1 cgacatccag cccttccacg tcatggaact gctgcgccgc gcgcgcgaac tcgaggcgca
       61 ggggcgcgac atcatccaca tggaggtcgg cgagcccgac ttcggcaccc cggcgccggt
      121 ggtcgaggcc gccactcgct tcctcgccgg cggcgacgtg cattacaccc cggcgctcgg
      181 cctgccggcg ctgcgtgagg cgatcgcgcg cttctatcac gaccgcttcg gcgccgacgt
      241 cgcgcccgag cgcatcgtcg tcaccgccgg cgcctcgggc gcgctgatgc tggcgctcgc
      301 cgccaccacc gatccgggcg acgagtggct gctgcccgac cccggctatc cctccaaccg
      361 tcacctgatc cgcgccttcg aaggggtcgc gcgcgcgctc gcggtcgatg ccgcgagccg
      421 ctaccagccc cgccccgaac aggtcgacgc ggcctggggc gagcgcaccc gcgggctgat
      481 ggtggcgaca ccgtccaacc ccaccggcac gctgctgtcg gtcgcggaga tcgccgcgct
      541 gcaccggcgc acccgcgcgc gtggcggcgt gctgctggtc gacgagatct accaggggct
      601 gacctacggc gtggaatcgg ccagcgcact tgccgacccg gtgctgaacg cggccgacga
      661 cgtcttcgtg gtgaacagct tctcgaagta tttcggcatg accggctggc ggctcggctg
      721 gctggtggcg ccggcgggct tcgtgcgcga gctcgagaag ctcgcccagc acttcttcat
      781 ctcgccgtcg acgccggcgc agcacgccgc gctcgcggcc ttcgcgccgg cgacgatcgc
      841 gatcctggag gagcgccgcc acgagtttgc ccagcgccgc gacgtgctgc tgccggcgct
      901 gcgcgcgctc ggcttcggca tcgccgcgga gccgcagggg gcgttctacg tgtatgcgga
      961 cgtgtcggcg ctcgccgacg actcggaagc gctcgcgcgc cggatgatcg aggaagcggg
     1021 cgtggccgcg acgcccggcc tggacttcgg ccaccacctg ccccgccgcc acctgcgcat
     1081 cgcgtacacc acgcacggcg cgcgactgct ggaggctgcg gagcggatcc gccggctggg
     1141 actgggaagc tgagccggag gccgctcgcc cgaggggtgg gcggtcggcg cctgcgaccg
     1201 gcgccaaact tcgaccggcg cccgaatcgc gacttgcgtc gctcctacgg caagccccgc
     1261 agagccccgg cgtaggatgc agcaacgccc cacgccctcg aaaccgcccc gcctgcgatg
     1321 taggagcgac gcaagtcgcg atcccccgcc gggcgcgacg taggagcgac gcaagccgcg
     1381 atcccccgcc gggcgcggcg taggagcgac gcaagtcgcg atcccccgtc cgacgccccg
     1441 cccgcgcgct tgcagggcgc aggaactcag ctcgcctgct ccgcgctgga acgatcgcgg
     1501 ccggcgatca gcatcaggcg cttcttcagc gccatgacct tgcgcgtgta gcgtgccttc
     1561 gggtcgttga gcgcaccgtt gtagtactgc agcgcgcgct gcatgctgcc gtagcgctgc
     1621 agcccctcgt gcagcaccag ggtgccgacg cggacattga cctcgggatc gaacaggacg
     1681 ttgcggccgc gattgggacc gatcttgtcc ttgtgatagc gcggaatcac ctgcatcaac
     1741 ccgagcgcgc ccatgttgct gaccgcgcga tggttgaagc tcgactccac cgccatgatc
     1801 gcgacgatca gcagggggtc gaaaccgagt tcctcggcgt gcgtctcggc cgcggccagc
     1861 gcgggctcga tcgtctcctc ggagacctgg taggtgtcgg cgatccagtc gcgcacacgc
     1921 tccatctcgt ccgagagcgc gccctcgtcc tcgaatgggt tgtcgaccag cgccgcgatg
     1981 tcgcgcagcg caggctggtg gaagaccggc gggatcggct ccgccacggc gcgcgcggcc
     2041 ttgcgctcga tcagccccgg cgcggccacc ggcaagctct cgtgcaagcg ggtggaggcg
     2101 gcgagggacg gaatcgaggc cgcgaaggaa ggcgccgaag cgcccatctc gccccggcgg
     2161 ggggcaaagg acagcaccga ggccatggcc agttcggaag cggtcggttc ggcctgcagg
     2221 ccgaggacgt cgccgtagcg cacatccgcg gcaccggcgc cttgcgtgcc gagccagccc
     2281 atacccatga gtccaacgga aagaaccgca ccgtgcgcca gactgcgcgc agcgccgccc
     2341 gcctgacgcg cgagcgcctt gaatcgagtt gcgtgtttca tggtgtcctc catgctgtgg
     2401 cctgagtcct gccgtggaag ggccctgtgg tgggggccgc ccggtgccgc cctgtcgggc
     2461 agcatccgcg tcgctcggcg ggttggccgg cacgacgggc aggagtactc ggtcgccagt
     2521 aggtgttaag acccatcgcc gcaaccagac gacgggcccc gcggtgcgtg cgtccatgaa
     2581 actgcgtctc
//
LOCUS       DAOQNN010000444         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_30879_length_2569_cov_13.515513, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000444 DAOQNN010000000
VERSION     DAOQNN010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30879_length_2569_cov_13.515513"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /gene="rpoD"
                     /locus_tag="PKV97_15885"
     CDS             <1..743
                     /gene="rpoD"
                     /locus_tag="PKV97_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNB07415.1"
                     /translation="AKMRRAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLM
                     KAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQIL
                     QETGQEPDPATLAEKMEMPEEKIRKIMKISKEPISMETPIGDDDDSHLGDFIEDTATL
                     APAEAAMYSGLRDATCEVLDSLTQREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERI
                     RQIEAKALRKLRHPSRSEKLRSFLDSDA"
     gene            786..861
                     /locus_tag="PKV97_15890"
     tRNA            786..861
                     /locus_tag="PKV97_15890"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:819..821,aa:Ile,seq:cat)
     gene            1068..1982
                     /locus_tag="PKV97_15895"
     CDS             1068..1982
                     /locus_tag="PKV97_15895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HNB07416.1"
                     /translation="MPIFTRGRHAPCFTPFETRSSGSVTRDPKGVMIMQVKPLCAALT
                     LAVLASTASAATTTLSGTIRDFCAPSITGTTGATTCTQLADFEGAIPGVVPNMTGTTL
                     SGGLPVAGANIVAGASTAANFAKWYVDSPGYNLSQAFSLTLGETTPGSGVYSYASSSF
                     FPIDGQLFGNQGRGHNYHFTLHLEGQLSFADPTAAADNSFSFTGDDDLWVFVNGIRFI
                     DLGGVHGAASASFTEETLKTAGLAPGTAYDLDIFFAERHTSESNFNITTSFAITPPSN
                     NVPEPGGLALLALGLAGLGAMRRRSPTR"
     gene            complement(2099..2426)
                     /gene="ssrA"
                     /locus_tag="PKV97_15900"
     tmRNA           complement(2099..2426)
                     /gene="ssrA"
                     /locus_tag="PKV97_15900"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01850"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="betaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01850"
BASE COUNT          505 a          900 c          737 g          427 t
ORIGIN      
        1 aagccaagat gcgtcgcgcc aagcgcgaga tgaccgaggc caacctgcgc ctggtaatct
       61 cgatcgcgaa gaagtacacc aaccgcggcc tgcagttcct cgacctcatc caggaaggca
      121 acatcggcct gatgaaggcg gtggacaagt tcgaataccg ccgcggctac aagttctcga
      181 cctacgccac gtggtggatc cgccaggcca tcacgcgctc gatcgccgac caggcgcgca
      241 ccatccgcat cccggtgcac atgatcgaga cgatcaacaa gatgaaccgc atcagccgcc
      301 agatcctgca ggagaccggt caggagccgg atcccgcgac gctggccgag aagatggaga
      361 tgcccgagga gaagatccgc aagatcatga agatctccaa ggagccgatc tccatggaga
      421 cgccgatcgg cgacgacgac gactctcacc tgggcgactt catcgaggac accgccaccc
      481 tggccccggc cgaggcggcg atgtactccg gcctgcgcga cgccacctgc gaggtgctcg
      541 actcgctgac ccagcgcgag gccaaggtgc tgcgcatgcg cttcggcatc gagatgaaca
      601 ccgaccacac gctggaagaa gtcggcaagc agttcgacgt cacccgcgag cgcatccgcc
      661 agatcgaagc caaggccctg cgcaagctgc gccacccgag ccgctccgag aagctgcgca
      721 gcttcctcga cagcgacgcg taaaggcccg gtgcaccgcc cggcggcgcc tgccgtcggg
      781 catgcgggcc tttagctcaa cggttagagc agaggactca taatcctttg gttgcgggtt
      841 cgaatcccgc agggcccacc aacgttttca agcacaacgc cagtcctccc cggggctggc
      901 gttgtgcttt tcgggtctcc cacattccgt ccaaggcagc gtcgtactgg aaggggcact
      961 ggcgcgtgcc gccgatcgag tcgttgtcgg cgttttttac agttccgccc ggatcacgcc
     1021 agccaatcac cttgcccgca agaatttact attttccatt caacaacttg ccgattttca
     1081 cccgaggcag gcatgcccct tgctttacac ccttcgagac ccggagttcc gggagtgtca
     1141 cacgggaccc gaaaggagtg atgatcatgc aagtcaaacc tctctgcgca gcgctgacgc
     1201 tggcagtgct cgcctccacg gcatcggccg ccacgacgac gctgtccggc accatccgcg
     1261 atttctgcgc gccatcgatc acgggcacca cgggcgctac gacatgcacc cagctcgccg
     1321 atttcgaagg cgccatcccc ggggtggtgc cgaacatgac cggaacgacc ctgagcggcg
     1381 ggctccccgt cgccggcgcg aacatcgttg ccggagcttc gacggcggcc aacttcgcga
     1441 aatggtacgt ggacagcccg ggctacaacc tgagccaggc cttctcgctg acgctcggcg
     1501 aaaccacgcc cggaagcggc gtctattcgt acgcctcgtc gagcttcttc ccgatcgacg
     1561 gccaactctt cggcaaccag gggcgcggcc acaactacca cttcacgctg cacctcgagg
     1621 gccagctctc ctttgccgac cccaccgccg ctgcagacaa ttcattcagc ttcaccggcg
     1681 acgacgacct gtgggtgttc gtgaatggca tccggttcat cgacctcggt ggcgtgcatg
     1741 gcgccgcaag cgcctccttc accgaggaga cgctgaagac cgccggactg gcaccgggaa
     1801 cggcctacga cctggatatc ttcttcgccg agcgccacac cagcgagtcg aatttcaaca
     1861 tcaccacgag cttcgcgatc acgcctcctt cgaacaacgt gcccgagccg ggagggctcg
     1921 cgctgctcgc cctcggactc gccggccttg gggcaatgcg acgccggtct cccacgcgct
     1981 gaagccgcgc ccgcaacggc cgcccgccgg gcggtcgttc ttcgtgaagt tccggagaaa
     2041 acgcagcctc gacgcgcgca ccgtcgccga atacagggaa atgcggaccg cgtagacgtg
     2101 acgatgggga ccgaagtccc caccggcttt tctttggtaa caaggctcaa gcagcctcgg
     2161 cataggcctt aagcggccag tgcgaaacgc tcatcgttgg cgtttatgga tttgcgcgga
     2221 ttacgtccgt cgcctctcgg gttgcctgct cacctttgcc cgccctgtcg aaaccggtac
     2281 acccccaccc aagcacactc ccgaatgcac ttgggtggag gtgaggggaa tcgaacccct
     2341 gtccagaacg cctccagatt gccgggatta caaccatgcc cgcgatctta cgcaggtcgg
     2401 cggctgcatt caatgcctgt gcgaggacaa ggcccgatcc gtttgtaagc aattgcaccg
     2461 cgccggacga actccctatc cgacgcggct ccagcccgca cgcacctcgc acctcgcacc
     2521 tcgcacctca cgccttcggc accctcccca tcaggtagaa gggctcgtt
//
LOCUS       DAOQNN010000445         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_31043_length_2562_cov_10.850020, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000445 DAOQNN010000000
VERSION     DAOQNN010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31043_length_2562_cov_10.850020"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2134)
                     /locus_tag="PKV97_15905"
     CDS             complement(<1..2134)
                     /locus_tag="PKV97_15905"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNB07417.1"
                     /translation="MSETTSASTQNTVKASAANLPMQEISGEVLVEKYAKGDEQTVAE
                     VRRRVARALAAVEAEDKRAHWEAKFLEAQEKGFVPAGRINSAAGTTLAATLINCFVQP
                     VGDSVTEAVDGRPGIYTALAQAAETMRRGGGVGYDFSSIRPKGALVKGTASNASGPVS
                     YMRVFDRSCETVESAGARRGAQMGVLRCDHPDIEEFIHAKDEGDLTNFNISVGVTDPF
                     MQAVEADGDVELVHKAEPTEEVKAAGAYQRDDGMWVYRKVRARELWDQVMRSTYDHAE
                     PGILFLDRMNADNNLYYCETIEATNPCAEQPLPPYGCCCLGSINLTLFVKNPFGDKAE
                     FDYAGFGKVVDVSIRMLDNVLEVTHWPLAQQHAEAQSKRRVGLGFTGLGDALIMLGQR
                     YDTAEAREEARKISEFMRNRAYLASSELARERGAFPLFNADLYLSGGNFASRLPADVK
                     DKIRKHGIRNSHLLSIAPTGTISLAFADNASNGIEPPFSYTYTRRKRMADGTFKEYAV
                     EDYAWRLYKHLGGNVDKLPQSFVTALEISAQAHKDMVAAVAPYVDTSISKTVNVPEDY
                     PYAEFEDLYLTAWKAGLKGLATYRPNSVLGSVLSVTPTAEQKQPHDVEIADANKRLSI
                     KNLPAPVLSSLRWPGRPDLPDGNLAWTYMLNTPTGGFALFVGQVGNNGGSFPFEVWVN
                     GADQPRGLGAVAKTLSMDMRANDRGWLKL"
     gene            complement(2350..>2562)
                     /locus_tag="PKV97_15910"
     CDS             complement(2350..>2562)
                     /locus_tag="PKV97_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB07418.1"
                     /translation="GAYAAEAATINLARRIAEADELDLPSSAAAARVAPETWGLLGLE
                     AERLAGLREGAEIDFAAYLSLFFPRR"
BASE COUNT          381 a          776 c          941 g          464 t
ORIGIN      
        1 tgagcttgag ccagccgcgg tcgttggcgc gcatgtccat cgacagggtc ttggccaccg
       61 cacccaggcc gcgcggctgg tcggcgccat tgacccacac ctcgaacggg aagctgccgc
      121 cgttgttgcc cacctggccg acgaagagcg cgaagccgcc ggtcggggtg ttgagcatgt
      181 aggtccacgc caggttgccg tcgggcaggt ccgggcggcc gggccagcgc aggctggaca
      241 gcaccggagc gggcaggttc ttgatcgaca ggcgcttgtt ggcgtcggcg atctcgacgt
      301 cgtgcggctg cttctgctcg gcggtcgggg tgaccgacag caccgaaccc agcaccgagt
      361 tgggacggta ggtggcgagc cccttgaggc cggctttcca ggcggtgagg taaagatcct
      421 cgaactcggc gtaggggtag tcctcgggca cgttcaccgt cttggagatg gacgtgtcga
      481 cgtagggcgc gaccgcggcg accatgtcct tgtgggcctg cgcggagatc tccagcgcgg
      541 tgacgaagga ctgcggcagc ttgtcgacgt tgccgccgag gtgcttgtac agacgccagg
      601 cgtagtcctc gaccgcgtac tccttgaagg tgccgtcggc catgcgcttg cgccgggtgt
      661 acgtatagga gaagggcggc tcgatgccgt tggaggcgtt gtcggcgaag gccaggctga
      721 tcgtgccggt gggggcgatc gacagcaggt gcgagttgcg gatgccgtgc ttgcggatct
      781 tgtccttcac atcggccggc aggcgcgagg cgaagttgcc gcccgacagg tagaggtcgg
      841 cgttgaagag cgggaaggcg ccgcgctcgc gggccagctc ggacgaggcc aggtaggcgc
      901 ggttgcgcat gaactcggag atcttgcgcg cttcctcgcg cgcctcggcg gtgtcgtagc
      961 gctggccgag catgatcagc gcgtcgccca ggccggtgaa tccgaggccc acgcggcgct
     1021 tggactgcgc ctcggcgtgc tgctgcgcga gcggccagtg ggtgacctcg agcacgttgt
     1081 cgagcatgcg gatggagacg tcgaccacct tgccgaagcc ggcatagtcg aactcggcct
     1141 tgtcgccgaa ggggttcttg acgaagagcg tgaggttgat cgagcccagg cagcagcagc
     1201 cgtagggcgg cagcggctgt tcggcgcagg ggttggtggc ctcgatggtc tcgcagtagt
     1261 acaggttgtt gtccgcgttc atgcggtcga ggaacaggat gcccggctcg gcgtggtcgt
     1321 aggtcgagcg catgacctgg tcccacagct cgcgcgcgcg caccttgcgg tacacccaca
     1381 tgccgtcgtc gcgctggtag gcgccggcgg ccttcacctc ttcggtgggc tcggccttgt
     1441 gcaccagctc cacgtcgccg tcggcctcga ccgcctgcat gaaggggtcg gtgacgccga
     1501 ccgagatgtt gaagttggtg agatcgcctt catccttggc gtggatgaat tcctcgatgt
     1561 cggggtggtc gcagcgcagc acgcccatct gcgcgccgcg gcgcgcgccg gcggactcca
     1621 ccgtctcgca cgagcggtcg aacacgcgca tgtaggacac cgggccggag gcgttggagg
     1681 ccgtgccctt gaccagcgcg cccttgggac ggatggacga gaagtcgtag ccgacgccgc
     1741 caccgcggcg catggtctcg gcggcctggg cgagcgcggt gtagatgccg gggcgaccat
     1801 cgaccgcctc ggtgaccgag tcgccgaccg gttgcacgaa gcagttgatc agcgtggcgg
     1861 cgagcgtggt gccggcggcg ctgttgatgc gaccggcggg cacgaagcct ttctcctgcg
     1921 cctcgaggaa cttcgcctcc cagtgggcgc gcttgtcctc ggcttcgact gcagccagcg
     1981 cgcgcgcgac gcggcgacgc acttcggcga cggtctgttc atcgcccttg gcgtacttct
     2041 cgacgagcac ctcgccggag atttcctgca tcggcaggtt ggcggcggag gctttgacgg
     2101 tgttctgggt ggacgcgctg gtggtctcgc tcatggtctt tttcttcctc cgtggtgggt
     2161 cttgggacgg acggaaggat agcgcggaac gagtgctggt gcaaaaaaat aaaaatatta
     2221 aaaaacaacg acttatataa tctctttgta tttcatgatt ttgcagggca ctacatgtag
     2281 tagcttccga agtggggtct ttgggctgga atcccagtct ccatgcgggt ttgcgccacg
     2341 ctgcggcccc tacctgcgtg ggaagaacag cgacaggtag gcggcgaaat cgatctccgc
     2401 accttcgcgc aggcccgcca ggcgctccgc ctcgagcccg agcaggcccc aggtctcggg
     2461 cgcgacgcgg gcggcggcgg cgctgctcgg gaggtccagc tcgtcggcct cggcgatgcg
     2521 gcgggcgagg ttgatggttg ccgcctcggc ggcgtaggcg cc
//
LOCUS       DAOQNN010000446         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_31279_length_2552_cov_9.983580, whole genome shotgun sequence.
ACCESSION   DAOQNN010000446 DAOQNN010000000
VERSION     DAOQNN010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31279_length_2552_cov_9.983580"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..640
                     /locus_tag="PKV97_15915"
     CDS             284..640
                     /locus_tag="PKV97_15915"
                     /inference="COORDINATES: protein motif:HMM:NF012329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HNB07419.1"
                     /translation="MSDMQDYHSRLSDPASRKFETFSYLPEMSTEQIRAQVEYIVKKG
                     WNPAVEHTEPENAFDHFWYMWKLPMFGESDVSRILAEAEACHKANPDHHVRLVGYDNF
                     KQSQGASMVIYRGKTV"
     gene            820..1644
                     /locus_tag="PKV97_15920"
     CDS             820..1644
                     /locus_tag="PKV97_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNB07420.1"
                     /translation="MHMADADPSGALAQYRINREPYYLPVADEIALFEAAYAARMPMM
                     LKGPTGCGKTRFIEHMAWRLGKPLITVACNEDMTASDLVGRFLLDAGGTRWQDGPLAL
                     AARHGAICYLDEVVEARQDTTVVIHPLTDTRRVLPLEKKGELVQAHADFQLVISYNPG
                     YQSLLKDLKQSTKQRFAALDFGYPEHAVECRIVAHEAGVSGDVADKLVSIAERARNLK
                     GHGLDEGISTRMLIHAGNLIARDVSPVTACRVALVRPITDDPDMRDALDAAVTTYF"
     gene            1722..>2552
                     /locus_tag="PKV97_15925"
     CDS             1722..>2552
                     /locus_tag="PKV97_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07421.1"
                     /translation="MDLGEYPELLDDLGPHAGEVLRASWTEASRVLSRTGLANYLRGA
                     QALHALGRGSDPVANFIQEAPAVAREVGEDVLADLVQGALAMASKTSGAVIALMLATA
                     PVAARRLGDAELFRAYLSFLGQLLAQAPRGVRPMLEQLEPLLGQLTLGGLRRWALWGA
                     QAHRVDFAAQERYFSLSSPDSQSVLQQERKGTLFVDVQRRIGMYLRALWGRDFFMRPT
                     STDYEARDGGRPFIDNWTIHLADAYDYTVELPLREGQCPPCQLGVNRHGVGLGHTTWL
                     K"
BASE COUNT          474 a          850 c          785 g          443 t
ORIGIN      
        1 tggaagcttg cgtcgaggcg cgcaaccagg gcgtggcgat cgagaaggaa ggcaaggcgg
       61 tcctgaccaa ggcggcccag tccagccccg agctcaaggt ggcgatggaa acctggaagg
      121 aaatcaagtt cgagttcgac acggtcgaca agctcgacgt ggcgcaccgt tgatcttccg
      181 acacggcgtt taaggcctaa agcattacga aacacgagag aacattcgga cgtccgggca
      241 aggcctctcg tccgccttca gaaatcgact tagaggaatt gccatgagcg acatgcagga
      301 ctaccattcg cgactgtccg atccggccag ccgcaagttc gaaacctttt cctacctgcc
      361 cgagatgagc accgagcaga tccgtgcgca ggtggaatac atcgtcaaga agggctggaa
      421 tcccgcggtc gaacacaccg aaccggagaa tgcgttcgac cacttctggt acatgtggaa
      481 gctcccgatg ttcggcgaaa gcgacgtcag ccgcatcctt gccgaagcgg aagcctgtca
      541 caaggcaaac cccgatcacc acgtacgcct ggttggttat gacaatttca agcagagcca
      601 gggcgcatcg atggtgatct accgcgggaa gacggtctaa ccgccgccgg accgctttcg
      661 aataccgacg cacgccgtgc gtctgccgat ccctctggct tgagggccgc aaggcccgat
      721 tgccatcctt ggccccgccc cgaaaggggt gggacttttt tcccgggatc cggtcggcag
      781 tttcgcatca cggcgctgca acacttcgcg gagcactcca tgcacatggc cgatgccgac
      841 ccatccgggg cactcgcgca atatcgcatc aaccgcgagc cctactacct gccggttgcc
      901 gacgagatcg cattgttcga ggccgcctac gctgcccgca tgccgatgat gctgaagggg
      961 ccgaccggct gcggcaagac gcgtttcatc gaacacatgg catggcgtct cggcaagccg
     1021 ctgatcaccg tcgcctgcaa cgaggacatg acggcttccg acctggtcgg ccgttttctc
     1081 ctcgatgccg gcgggacgcg ctggcaggac ggcccgctcg ccctggcagc ccgtcacggc
     1141 gcgatctgct acctcgatga agtcgtcgaa gcgcggcagg acacgacggt ggtgatacac
     1201 ccgcttaccg acacgcgtcg cgtgctgccg ctcgagaaga agggcgaact ggtccaggcg
     1261 cacgccgatt tccagctggt catttcctac aaccccggct accagtcgct gctcaaggat
     1321 ctcaagcagt cgaccaagca gcgcttcgcc gcgctcgact tcggctatcc ggaacacgcg
     1381 gtggaatgcc gcatcgtcgc acacgaggcc ggcgtatccg gcgacgttgc cgacaaactg
     1441 gtttcgatcg ccgaacgtgc gcgcaacctc aagggccatg gcctcgacga gggcatctcg
     1501 acacgcatgc tgatccatgc cggcaacctg attgctcgcg atgtctcccc ggtcacggcg
     1561 tgccgcgtgg cgctggtgcg gccgatcacc gacgaccccg acatgcgcga cgcgctcgac
     1621 gccgccgtga ccacgtattt ctgatgtgcc ggcgggtcgg atgaattctc gtcactggaa
     1681 agcggcacgc tgtcgcgcga tgacagcctc cgactgaact aatggacctc ggcgaatacc
     1741 cggagctgct cgacgacctc ggcccgcacg ccggcgaagt gttgcgtgct tcctggaccg
     1801 aggcatcgcg cgtcctgtcg cggacaggtc tcgcgaacta cctccgcggt gcgcaggctc
     1861 tccacgcact cgggcgcggc agcgacccgg ttgcgaattt catccaggaa gctcccgccg
     1921 tggcacgcga agtcggcgag gacgtgcttg ccgatctcgt ccaaggcgca ctcgcaatgg
     1981 cgtcgaagac cagcggcgca gtgattgcgc tgatgctggc aacggcgcct gtggcggctc
     2041 gacggctcgg ggatgccgaa ttgtttcgcg cctatctgtc gtttctcggc cagttgctgg
     2101 cgcaggcgcc gcgcggcgtg cgcccgatgc tcgaacagct cgaaccgctg ctcggccagt
     2161 tgaccctggg cggcctgcgg cgttgggcac tgtggggcgc ccaggcgcat cgcgtcgatt
     2221 tcgcggcgca ggagcgctac ttctcgttgt ccagcccgga ttcgcagtcg gtgctgcagc
     2281 aggaacgcaa gggaaccctg ttcgtcgacg tccagcgccg tatcggcatg tacctgcgcg
     2341 cactttgggg gcgtgatttt ttcatgcggc cgacctccac cgactacgaa gcgcgcgatg
     2401 gcggacgccc cttcatcgac aactggacta tccacctcgc cgacgcctac gactacaccg
     2461 tcgagctccc cctccgcgag ggccagtgcc ccccctgcca gctcggcgtc aaccgccacg
     2521 gcgttggcct cggccatact acatggctaa ag
//
LOCUS       DAOQNN010000447         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_31282_length_2552_cov_7.074489, whole genome shotgun sequence.
ACCESSION   DAOQNN010000447 DAOQNN010000000
VERSION     DAOQNN010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31282_length_2552_cov_7.074489"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="PKV97_15930"
     CDS             complement(<1..1066)
                     /locus_tag="PKV97_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNB07422.1"
                     /translation="MFGFLKKKFGKSVDEARPAEQAAEEQTASEVRAEDLAGAPAPQA
                     PAEASVAEAARRDAAEIDATAEPVEPAALPESPAQPAAEAAALAEPEPAAQAPAEAVP
                     EPQPEPPGEPTPEAPVAVEPAIEAESEAESEAESEAEPEAEPEPEPEPSAAAAAVAKK
                     SWTERLKAGLARTRNQIGGGLASLFGLRKIDEDLLEELESTLLMADCGVDATQHLIDD
                     LRRRWKRDRLETADQLQKALADGLHEIIAPLEAPLEVAGHRPFIIMIAGVNGSGKTTS
                     IGKLAKYFQAHGKSVLLAAGDTFRAAAREQLMTWGERNNVTVVAQDSGDAAAVIFDAI
                     NAARARKIDVVLADTAGRLPT"
     gene            1231..>2552
                     /locus_tag="PKV97_15935"
     CDS             1231..>2552
                     /locus_tag="PKV97_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB07423.1"
                     /translation="MFRLPPALRLARLPGSRLARGLLLGATLSAALAPPVLANPFETT
                     LANGMKLIVKEDRRAPSVVHMVWYRTGAMDEPDGVSGVAHVLEHMMFKGTREVGPGEF
                     NRRVAAVGGRDNAFTGKDYTAYFQQVPPAHLPAMMALEADRMKNLVLTDEEFAREIEV
                     VKEERRLRTDDQPRALVFEQLMATAYQAHPYRRPVIGWMPDLEAMRPEDARTWYRRWY
                     APNNAYLVVVGDVDHREVFRDAEQTYGALPAGELPARRISPEPAQRGPRASTVKAPAE
                     LPYLAMAWHVPALRDPANDREAFALDVLAAVLDGYDGARLTRGLVRDQRIAVSVGASY
                     DTGNRGPALFYLHGVPAAGTSPEALAEALRAELRRIADEGISAAELARVKTQAIAAQV
                     YKRDSLMGQAMEIGHLESAGLSWRDEDRLLDGLRAVTAEEVQAVAKRY"
BASE COUNT          334 a          949 c          902 g          367 t
ORIGIN      
        1 gggtgggcag gcggccggcg gtgtcggcga gcacgacgtc gatcttgcgc gcacgtgcgg
       61 cgttgatggc gtcgaagatc accgccgcgg cgtcgccgct gtcctgcgcc accacagtga
      121 cgttgttgcg ctcgccccag gtcatcagct gctcgcgcgc ggcggcgcgg aaggtgtcgc
      181 ccgcggcgag cagcacgctc ttgccatggg cctggaagta cttggccagc ttgccgatcg
      241 aggtcgtctt cccggagccg ttgacgcccg cgatcatgat gatgaagggg cgatgacctg
      301 cgacctcgag cggcgcttcg agcggcgcga tgatctcgtg caggccgtcg gcgagcgcct
      361 tctgcagctg gtcggcggtc tccaggcggt cgcgcttcca gcgccggcgc aggtcgtcga
      421 tcaggtgctg ggtggcgtcg acgccgcagt cggccatcag cagggtggat tcgagctcct
      481 cgagcaggtc ctcgtcgatc ttgcgcaggc cgaacaggct cgccagccct ccgccaatct
      541 ggtttcgagt gcgcgcgagg ccggccttga ggcgctcggt ccacgacttc ttcgcgaccg
      601 cggcggcggc cgcggacggc tccggctccg gctccggctc ggcttcgggc tcggcttcgg
      661 actcggcttc ggactcggct tcggactcgg cttcgatcgc cggctcgacc gcgacggggg
      721 cttcgggcgt cggctcgccc gggggttccg gctgcggctc gggcacggct tcggctggcg
      781 cctgcgcagc gggctccggc tcggcaaggg ccgccgcctc ggcggccggc tgcgcagggg
      841 attcgggcag cgcagcgggc tcgacgggct ctgcggtcgc gtcgatctcc gccgcgtcgc
      901 gacgcgcagc ctcggccacc gaggcctccg cgggcgcttg cggcgccggc gcgccggcga
      961 ggtcttcggc gcggacctcg gaggcggtct gctcctcggc agcctgctcg gcgggcctgg
     1021 cctcgtcgac agacttgccg aacttcttct tcaggaaacc aaacatgaaa cgctcgggtc
     1081 agaggggacg ttttgcgtcg gcgcacgcgg cccggcaagc atgggcgcgc accgccgatc
     1141 acgctaagat gcggctcccc ggcactggct gcgccatgcg cagcgggcct cgcggagtgc
     1201 cgcggattct aacagatgca ggacaactcc atgtttcgcc tcccccccgc cctccgcctc
     1261 gcccgcctgc ccggaagccg cctcgcacgc gggctcctcc tcggcgcgac gctctccgcc
     1321 gcgctcgccc cgcccgtcct cgccaacccc ttcgagacca cgctcgccaa cggcatgaag
     1381 ctgatcgtca aggaggaccg ccgcgcgccc tcggtggtgc acatggtgtg gtaccgcacg
     1441 ggtgcgatgg acgagccgga cggcgtctcg ggcgtggccc atgtgctcga gcacatgatg
     1501 ttcaagggca cgagggaggt cggcccgggc gagttcaacc gccgcgtggc cgccgtcggc
     1561 gggcgcgaca acgccttcac cggcaaggac tacacggcct acttccagca ggtgccgccc
     1621 gcccacctgc cggcgatgat ggcgctcgag gccgaccgca tgaaaaacct cgtgctcacc
     1681 gacgaggagt tcgcgcgcga gatcgaggtg gtcaaggagg agcgccgcct gcgcaccgac
     1741 gaccagccgc gcgcgctcgt gttcgagcag ctgatggcca ccgcctacca ggcccacccc
     1801 taccgccgcc cggtgatcgg ctggatgccc gacctcgagg ccatgcggcc ggaagacgcg
     1861 cgcacctggt accggcgctg gtacgcgccc aacaacgcgt acctggtggt cgtgggcgac
     1921 gtcgaccacc gcgaggtgtt ccgcgacgcc gagcagacct acggcgccct ccccgccggc
     1981 gagctgcccg cccgccgcat ctcccccgaa ccggcgcaac gcggcccgcg cgcctcgacg
     2041 gtgaaggccc ccgccgagct gccctatctc gccatggcct ggcacgtgcc cgcgctgcgc
     2101 gatcccgcca acgaccgcga ggccttcgcg ctcgacgtgc tcgccgccgt gctcgacggc
     2161 tacgacggcg cccgcctcac ccgcggcctg gtgcgcgacc agcgcatcgc ggtcagcgtc
     2221 ggtgccagct acgacaccgg caaccgcggc ccagcgctgt tctacctgca cggcgtgccc
     2281 gccgccggca ccagcccgga ggcgctcgcc gaggccttgc gcgccgagct gcgccgtatc
     2341 gccgacgaag gcatcagcgc cgcggagctc gcgcgcgtga agacgcaggc gatcgccgcc
     2401 caggtgtaca agcgcgactc gctgatgggc caggcgatgg agatcggcca cctcgaatcc
     2461 gcgggcctgt cctggcgcga cgaggacagg ctgctcgatg gcctgcgcgc ggtcaccgcc
     2521 gaggaggtgc aggcggtagc gaagcgttac tt
//
LOCUS       DAOQNN010000448         2541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_31519_length_2541_cov_14.833065, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000448 DAOQNN010000000
VERSION     DAOQNN010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31519_length_2541_cov_14.833065"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1392
                     /locus_tag="PKV97_15940"
     CDS             178..1392
                     /locus_tag="PKV97_15940"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB07424.1"
                     /translation="MTPLTDIELAALADDLESDRVERKRAWKGDAPEKGRQAVCAFAN
                     DLPNHRKPGVLLVGIADDGTPAGPEVVVTDALLQTLADIKTDGKITPPPTLTVTRRFL
                     KGHDVAVVTVWPADAPPVRLDGRIWIRTGPRRGLASAQDERILNERRRHLDRAFDATP
                     VYGCPLDELDRLTFEREFLPQAIAPDVLAANDRSYEDRLASLGMIASAADPTPTVAGI
                     LTLGKSPRTWVPCAYIQFLRIRGTELADPIADAAELDGTLQQMLRRADEKLAAFLTTE
                     VDFTSATIEERRTPYPMSALQQLLRNAVMHRTYEGTNAPVRLYWFDNRIEIHSPGGPY
                     GVVNAANFGQPGACDYRNQILAGVLKVLGFVQRFGFGIAQARRALAANGNPPPEFRVE
                     PTMVLATLRRAP"
     gene            1389..2402
                     /locus_tag="PKV97_15945"
     CDS             1389..2402
                     /locus_tag="PKV97_15945"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB07425.1"
                     /translation="MSTPVLTFFNNKGGVGKTSLVFHVAWMLCELGKRVVAIDLDPQA
                     NLTSAFLAEETLETLWDPPAPVESGTTIFRCVQPLTKVGDIRPPVVQSIHPGLVLVPG
                     DLALAGFEDELSKAWLEAMGSNNLYRPFRLLTAFWQVAQMAAAEHEADLILADVGPNL
                     GAINRSALIGSDHVVIPLAADLFSLQGLRNLGPTLTAWRSDWQKRLNNWPEPEFLLPA
                     GRMAPLGYILMQHMERLSRPVKAYKKWVDRIPATYVETVTGVQSPTPAHADDGNCLAR
                     LKHYRSLIPMAQETRKPVFHLTSADGAIGSHSQAVRDAGNDFRALATTILQRIDRGEE
                     SAF"
BASE COUNT          459 a          859 c          767 g          456 t
ORIGIN      
        1 ggcgccaccg ccgcgatgct ggtcgagttc taccggcggc tggcggcctt catggtgggt
       61 gggggttaag cggatggatt actctatttt gtgtgaccca atcagttgtc ccgaccattc
      121 tcttatcgcc tcggccgcac tgacgacggc cttgcgcgaa tgccaggaga acgccacatg
      181 actcccctga ccgacatcga gctggccgcg ttggccgacg acctcgaaag cgatcgggtc
      241 gagcgcaagc gtgcctggaa gggcgatgca ccggaaaaag ggcgccaggc ggtctgcgct
      301 ttcgccaacg acttgcccaa tcaccgcaaa cccggtgtcc tgctggtcgg tatcgccgac
      361 gacggcacgc cggctggccc ggaggtcgtg gtcaccgacg cgcttctgca aaccctggcg
      421 gacatcaaga ccgacggaaa aatcacgccg ccacccacgc tgacggtcac caggcggttt
      481 ctcaaaggcc acgacgtggc cgtggtcaca gtgtggccgg cggatgcgcc accggtccgg
      541 ctggatggcc gcatctggat ccgaactggc ccaaggcgcg gcttggcctc cgcgcaggac
      601 gagcggatcc tgaatgagag gcgccgccat ctggaccggg cattcgacgc cacgcctgtg
      661 tacggctgcc cgctcgatga gctggatcga ctgacgtttg aacgtgagtt cctgccgcag
      721 gcgatagccc ctgacgtgct tgccgccaac gaccgcagtt acgaagatcg tctggcatct
      781 ctgggcatga tcgcgtcggc ggccgacccg acgcccacgg tcgccggcat tctcacactg
      841 gggaaatcgc cgcgcacctg ggtgccgtgc gcgtacatcc agttcctgcg catacgcggc
      901 accgaactgg ccgacccgat cgccgacgcc gcagaattgg atgggaccct gcagcagatg
      961 ctgcgcaggg cggacgagaa gctcgcggcc tttctgacca ctgaagtcga tttcacctcc
     1021 gccacgatcg aggagcggcg cacgccttac ccgatgagcg ccctgcagca gctgctgcgc
     1081 aacgcggtga tgcaccgcac ctacgaaggc accaacgccc cggtgcgcct gtactggttc
     1141 gacaaccgca tcgagattca cagcccgggc gggccgtatg gtgtcgtcaa cgcggcgaat
     1201 ttcggccaac cgggcgcctg cgattaccgc aaccagattt tggctggcgt cctcaaggta
     1261 cttggcttcg tacagcgttt cggcttcggt atcgcccagg cacgccgcgc gctcgcagcc
     1321 aatggcaatc caccacccga gttccgcgtg gaaccgacca tggttctggc aacccttcgc
     1381 agggcgccat gagcaccccg gttctcacct ttttcaacaa caagggcggc gtcggcaaga
     1441 cctcgctggt tttccacgtt gcctggatgc tctgcgaact gggcaagcgc gtggtcgcca
     1501 tcgatctcga cccccaggcc aatctgacat cggcctttct cgcagaagag acgctggaaa
     1561 ccctgtggga cccaccggct ccagtcgagt ccggcacgac gatcttccgc tgcgtgcagc
     1621 cgctcaccaa ggtcggcgac atccgtccgc cggtggtgca gtccatccat cccgggctgg
     1681 tgctggtgcc cggcgatctg gcattggccg ggttcgagga cgagctgtcc aaggcgtggc
     1741 ttgaggccat gggttcgaac aacctctatc ggcccttccg cctgctcacc gccttttggc
     1801 aggtggcaca aatggccgct gccgaacacg aagccgatct gatcctggcc gacgtcgggc
     1861 cgaacctggg cgccatcaac cgttcggccc tgatcggcag cgatcatgtg gtcattccgc
     1921 tagcggccga tctgttctcg ctgcagggcc tgcgcaacct cggccccaca ttgaccgctt
     1981 ggcggtccga ctggcaaaag cggctgaaca actggccgga gccggagttt ttgctgccgg
     2041 ctggccgcat ggcgccactg ggttacatcc tgatgcagca catggagcgc ctgtcacgtc
     2101 cagtgaaggc ttacaaaaaa tgggtcgacc ggattccagc tacgtatgtt gaaaccgtca
     2161 cgggtgtgca atctccgaca cccgcccacg ctgacgacgg caattgcctg gctcgactca
     2221 agcactaccg cagcctgatc cccatggccc aggagacgcg caagcccgtg ttccatctca
     2281 cctcggcgga tggcgcaatc ggcagccatt cgcaagccgt ccgcgacgca gggaacgatt
     2341 ttcgggcgct cgcgaccacc attctccaac gcattgaccg gggcgaagaa tcagccttct
     2401 gacgcttgct tctctcctgc caagcaatac ctccagtaac accgacggag tcggcctgtt
     2461 cctgatccaa agtcgccgca ggcttttcta aaccaccccc gcctgcttca gccactgggc
     2521 cagttgcggt ggtgacaggg c
//
LOCUS       DAOQNN010000449         2535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_31661_length_2535_cov_8.237500, whole genome shotgun sequence.
ACCESSION   DAOQNN010000449 DAOQNN010000000
VERSION     DAOQNN010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31661_length_2535_cov_8.237500"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..389
                     /locus_tag="PKV97_15950"
     CDS             102..389
                     /locus_tag="PKV97_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB07426.1"
                     /translation="MRLREERGLSLRALSERSGIPVGTLSWWTHHLRASASRTDFVAV
                     KIRDDEDGRDPPSGCGASRLRMRLPSGVSLTVEGELAERVVAAVMEGMQRW"
     gene            383..817
                     /gene="tnpB"
                     /locus_tag="PKV97_15955"
     CDS             383..817
                     /gene="tnpB"
                     /locus_tag="PKV97_15955"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HNB07427.1"
                     /translation="MVIALPTGTTVHLAREATDMRKAFDGLCNLVRFGFDLDPFTGSL
                     FVFFNRRATHVKILQWDGNGFWLHAKRLERGTFERWHPSSDGARHVEIDRAQLMMLLE
                     GIDLKKVKFRRHFARSVRIDGGGRIEPDEARRRAADRRQPAR"
     gene            747..2372
                     /locus_tag="PKV97_15960"
     CDS             747..2372
                     /locus_tag="PKV97_15960"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HNB07428.1"
                     /translation="MAVAGSNQTKHVGAQQIDVNQLGDVELRDLVGKLMGEVARLQRD
                     NSALRRMLFGRRSERTKFVIGPGMLPFAEFEELKVDLERATAQAKQVEIATHTRNVNT
                     RRSELPAHLPRERVEHTLPEAERACPECGTTRQEIGEDVTQELERLEICFVREIAQKK
                     YACRACQGHVVKAKAPGRVLEKCLLGPAFLAQVAFDRFANHMPYARLERKYAVEGADL
                     SRSVLCSSMARLAGHLKPVFAVHTDETIDAITTSVLQVDDTEVLQRNGNSPGGRKVHV
                     WAWRNQDGGVVFHVTAERNQDTPRRMLGARTGLLQGDGHDCYKKLGDGVVLLGCMAHA
                     RRKFEAALKGGNTLAREPFEWMNMLFAIEREAKALGISDDEARLLEHRRAKSVPILTA
                     LRAWIDDASLRQIDLPQSPLMDAVGYCRNQWVPLTRFAENGRIREISNNACENTLRNV
                     VLGRKNWLFFGNEDGAENSMLLLSLIQSCRELGINPLAYLRDVMSRVHETEPDRMHEL
                     TPRGWRSAADHDAKIAAARRDLAELVARLQFDA"
BASE COUNT          443 a          812 c          858 g          422 t
ORIGIN      
        1 cgtaactgtc cggggaacca catctgtttc cgcgccggca cttcgccacg ctttccggca
       61 tggcaagtcg tcaaagtcga agagcagaga tcgagcgttg gatgcggctg cgtgaagagc
      121 gcgggctgag cctgagggcg ctgtcggagc gatcagggat cccggtgggc acgctgtcct
      181 ggtggactca tcacctccgt gcctcggcat cgcggacgga cttcgtcgcg gtgaagattc
      241 gcgacgacga ggacggccgt gatccgccat ctggatgcgg agcatcgcgg ctgcgcatgc
      301 ggttgccctc gggagtgtcc ctgacggtcg agggcgagct tgccgagcgc gtggtcgcgg
      361 ccgtcatgga gggcatgcag cgatggtgat cgcgttgccg accggcacga cggtccatct
      421 ggcgcgggag gcaaccgaca tgcgcaaggc cttcgacgga ttgtgcaacc tggtgcgctt
      481 cggtttcgat ctcgatccgt tcacgggcag cctgttcgtc ttcttcaatc gccgcgccac
      541 ccacgtgaag atcctgcagt gggatggcaa cggcttctgg ctgcacgcca agcgtctcga
      601 gcgcggcacc ttcgaacgct ggcatccgag ttcggatggc gcacgtcacg tcgagatcga
      661 tcgcgcacaa ctgatgatgc tgctcgaggg gatcgacttg aagaaggtga agtttcggcg
      721 ccactttgcg cgtagtgtgc gtatcgatgg cggtggccgg atcgaaccag acgaagcacg
      781 tcggcgcgca gcagatcgac gtcaaccagc tcggtgacgt cgagctgcgc gatctcgtcg
      841 ggaagctgat gggtgaagtc gcgcggctgc agagggacaa ctcggcgttg cgccgcatgc
      901 tcttcggccg ccgctcggaa cggaccaagt tcgtgatcgg cccggggatg ctgcccttcg
      961 ccgagttcga ggaactaaag gtcgatctcg agcgcgcgac agcgcaggcg aagcaggtcg
     1021 agatcgctac ccacacccgc aacgtcaaca cgcgtcgctc ggagttgccc gcgcatctgc
     1081 cccgcgaacg ggtcgagcac acgctgccgg aggccgagcg cgcgtgtccg gagtgcggca
     1141 cgactcgcca ggagatcggc gaggatgtca cccaggaact cgagcggctg gagatctgct
     1201 tcgttcggga gatcgcgcag aagaagtacg cctgccgcgc ctgtcagggt catgtggtca
     1261 aggcgaaggc tccgggccgg gtgctggaga agtgcctgct cggccccgcg ttcctggcgc
     1321 aggttgcatt cgaccgcttc gccaaccaca tgccgtatgc ccggctcgag cggaagtacg
     1381 cggtcgaggg cgccgacctc tcccgtagtg tgctgtgcag ttcgatggcg cggctcgcgg
     1441 ggcatctgaa gccggtcttc gcagtgcaca ccgacgaaac catcgacgcg atcaccacct
     1501 cggtactgca ggtcgacgac acagaagtcc tgcaacgcaa cggcaactct cctggcggcc
     1561 gcaaggtcca tgtctgggcc tggcggaacc aggatggagg cgtggtcttc catgtcaccg
     1621 cggaacgcaa ccaggacact ccgcggcgca tgctcggcgc tcgaacagga ctgctgcagg
     1681 gcgacggcca cgactgctac aagaagctcg gtgatggcgt cgtcctcttg ggatgcatgg
     1741 cccacgcgcg tcggaagttc gaggccgcac tcaagggcgg caacactctg gctcgcgagc
     1801 ccttcgagtg gatgaacatg ctgttcgcca tcgaacgcga ggcgaaggcg ctcggcatca
     1861 gtgacgacga agcgagactg ctcgaacatc gccgcgcgaa gtcggtgccg atcctgactg
     1921 ccttgcgcgc gtggatcgac gacgcctcgc tgcgccagat cgacctcccc cagagtccgc
     1981 tgatggatgc ggtcggctac tgccgcaacc agtgggtgcc tctgacccgc ttcgccgaga
     2041 acggcaggat ccgcgagatc tcgaacaacg cgtgcgagaa cacgctgcgc aacgtggtgc
     2101 tcgggcggaa gaactggctg tttttcggga acgaggatgg tgctgagaac agcatgctct
     2161 tgctcagcct gatccagtcg tgccgcgaac tcggcatcaa tcctctcgcc tacctccgcg
     2221 atgtgatgag tcgcgtgcac gagaccgagc cggatcggat gcacgaactc actcccagag
     2281 gatggcggtc ggccgccgac cacgacgcga agatcgccgc cgcgcggcgc gatctcgccg
     2341 agctcgtggc ccgcttgcag ttcgacgcct gatcgacgcc gtcgaccgcg cggtgccggg
     2401 caccgccgtc ccgactctcc agggtgcgcc gccgcccggc tggactgggc ggggcacccg
     2461 ccaggtcgcg aacacgcgcc agcctcctcg cgctcacgcg ccgcgagtcc ggggtgcact
     2521 tccccggaca gttac
//
LOCUS       DAOQNN010000450         2525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_31898_length_2525_cov_11.031984, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000450 DAOQNN010000000
VERSION     DAOQNN010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31898_length_2525_cov_11.031984"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PKV97_15965"
     CDS             complement(<1..720)
                     /locus_tag="PKV97_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB07429.1"
                     /translation="MSSTASYVHGASAKPLIGQTIGRFFDEACARHAGREALVVRHQD
                     VRLTYAQLKSKVDALACGLMRLGLEPGERIGIWSQNNMEWALTQFASAKAGLVLVNIN
                     PAYRRSELEYALEKVGCRALILSPAFKTSNYLEMIADLAPELGHCEPGLLRSHRLPSL
                     EMVIRMGAEKSPGMMSFDDLLRAPSREELTALAVLSEKLQFDDPINIQFTSGTTGHPK
                     GATLSHHNILNNGFFVGEAIKL"
     gene            complement(1183..>2525)
                     /locus_tag="PKV97_15970"
     CDS             complement(1183..>2525)
                     /locus_tag="PKV97_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-methylcrotonyl-CoA carboxylase"
                     /protein_id="HNB07430.1"
                     /translation="VYLFERDCSVQRRHQKVLEEAPAPGMTLERRAAMGKAAVDAAKA
                     VGYVGAGTVEFIANQDGSFYFMEMNTRLQVEHPVTEMITGLDLVEWQLRVASGEKLPL
                     AQEQLQIRGHALEARIYAEDPAKGFLPSTGKLVHLAPPAESLHVRVDTGVEEGDEISP
                     HYDPMIAKLIVWDINRDRALARMLQALADYRVVGVANNIEFLSRLTACPAFAGADLDT
                     GLIEREKAYLFPADEAVPAEVLQIAALAELLRDAAFADRRAARGADPASPWHARDGWR
                     MNATACRSLLFRHGESERAVEVAYLPGAWRLTVGSSSVVARGELNPRGLLRVELDGTR
                     MDATVIAAAGRRHVFARGRAWQLAAVDPLHHGGEGGGAEGGLMAPMPGKVIALVAAEG
                     AKVEKGAPLLILEAMKMEHTITAPAAGTVKAFRFGVGDQVGDGAELVEFEAAAV"
BASE COUNT          351 a          891 c          894 g          389 t
ORIGIN      
        1 cagcttgatc gcctcgccga cgaagaagcc gttgttgagg atgttgtggt gcgacagcgt
       61 ggcgcccttg gggtggccgg tggtgccgga ggtgaactgg atgttgatcg ggtcgtcgaa
      121 ctgcagcttc tcggacagca cggcgagcgc ggtcagttcc tcgcggctgg gcgcgcgcag
      181 caagtcgtcg aagctcatca tgccgggcga cttctctgcg cccatgcgga tcaccatctc
      241 caggctgggc aggcggtggc tgcgcagcag gccgggctcg caatggccga gctcgggcgc
      301 aaggtcggcg atcatctcga ggtagttgct ggtcttgaag gcgggcgaca ggatcagcgc
      361 gcggcaaccg accttctcga gcgcgtattc gagctcggag cggcggtagg cggggttgat
      421 gttcaccagc accaggccgg ccttggcgct ggcgaactgg gtcagcgccc actccatgtt
      481 gttctgcgac cagatgccga tgcgctcgcc cggttccagg ccgaggcgca tcaggccgca
      541 ggcgagcgcg tcgaccttgc tcttgagctg cgcgtaggtc aggcgcacgt cctggtgacg
      601 caccaccagc gcctcgcggc cggcgtggcg ggcgcaggcc tcgtcgaaga agcggccgat
      661 ggtctggccg atcaggggtt tggcggaagc gccgtgcaca tagctcgcag tgctgctcat
      721 cgtgtcgtcc tctctcctgt tcttgtcgtc gcgcggcatg tcgcgctcgc aatcctgtcc
      781 atcccgtgcc cgcgccacca cggaaccgct tcattcgcga cttgcgtcgc tcctacacgg
      841 agccccagac gcgcgcacgg tttccgtcat caccgaccca ccccatcgca agcgggcggg
      901 gagccccaca cgcgcgcggt tttccggcat cgccgacgca ccccatcgca ggtgggcagg
      961 aagcgccaga tgcccggcgc ttcccgtagg agcgacgcaa gtcgcgatcg ggcggctccg
     1021 cggcacaggc atggcgtcgg tgcgccctgc ggcatgcatg gacgcctcct gcggaaaacc
     1081 gccgcgcctc gggggccatg tgggagcgac gccagtcgcg attgccgccg ggcacccgcg
     1141 accgcgccga acaggtgcag gcgccccgcc acggcgttgg cctcaaaccg ccgcggcttc
     1201 gaactccacc agctcggcac cgtcgccgac ctggtcgccg acgccgaagc ggaaggcctt
     1261 gaccgtgccg gccgccggcg cggtgatggt gtgctccatc ttcatcgcct ccaggatcag
     1321 cagcggcgcg cccttctcca ccttcgcccc ctctgcggcg accagcgcga tgaccttgcc
     1381 cggcatcggc gccatcagcc cgccctcggc accgccgccc tcgccgccgt ggtgcagcgg
     1441 atcgaccgcg gcgagttgcc aggcgcggcc cctggcgaag acgtgacggc ggccggcagc
     1501 ggcgatcacg gtggcatcca tgcgggtgcc gtcgagctcg acccgcagca ggccgcgcgg
     1561 gttgagctcg ccgcgcgcga ccacgctgct gctgcccacc gtcagccgcc aggcgccggg
     1621 caggtaggcc acctcgaccg cgcgctcgct ctcgccgtgg cggaacagca agctgcggca
     1681 ggcggtggcg ttcatgcgcc agccgtcgcg ggcatgccag ggcgaggccg gatcggcgcc
     1741 gcgcgccgcg cggcggtcgg cgaaggcggc gtcgcgcagc agctcggcaa gcgcggcgat
     1801 ctgcaggacc tcggccggca cggcctcgtc ggctggaaac aggtaggcct tctcgcgctc
     1861 gatgagcccg gtgtcgaggt cggcgccggc gaaggccgga caggccgtga ggcgcgacag
     1921 gaactcgatg ttgttggcca cgcccaccac ccggtagtcg gcgagggcct gcagcatgcg
     1981 ggcgagcgcg cggtcgcggt tgatgtccca cacgatcagc ttggcgatca tcgggtcgta
     2041 gtgcgggctg atctcgtcgc cctcctcgac gccggtgtcg acgcgcacgt gcaggctctc
     2101 ggccggcggc gccagatgca ccagcttgcc ggtcgagggc aggaagccct tggccgggtc
     2161 ttcggcgtag atgcgcgctt ccagcgcatg gccgcggatc tgcagttgct cctgcgccag
     2221 cggcagcttc tcgcccgagg cgacgcgcag ctgccactcg acgaggtcca gcccggtgat
     2281 catctcggtg accgggtgct cgacctgcag gcgggtgttc atctccatga agtagaagga
     2341 gccgtcctgg ttggcgatga actccaccgt gcccgcgccg acatagccca ccgccttggc
     2401 cgcatcgacc gcggccttgc ccatcgcggc gcggcgctcg agcgtcatgc cgggcgcggg
     2461 ggcttcttcg agcaccttct ggtggcggcg ctgcaccgag cagtcgcgct cgaacaggta
     2521 cacca
//
LOCUS       DAOQNN010000451         2516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_32097_length_2516_cov_7.661926, whole genome shotgun sequence.
ACCESSION   DAOQNN010000451 DAOQNN010000000
VERSION     DAOQNN010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32097_length_2516_cov_7.661926"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="PKV97_15975"
     CDS             complement(<1..1271)
                     /locus_tag="PKV97_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNB07431.1"
                     /translation="MSLPSRLAFIDVESTGAHPVRDRITEIAILRVEDGEVVARWESL
                     VAPGQPIPPLIEEVTGISDAMVAEAPAFEVLADTVAALLEGCVFVAHSARFDYGFIRN
                     AFTRIGREFEAPVLCTVKLSRALYPEHHRHGLDALIERHGLVCAARHRAMGDVEVLWQ
                     FVRGLEAGFAPEVLARAAARAMKAPTQPPGLPEGMLEALPDAPGLYLFHAQPEPPPAG
                     ARDPALFVGRAQSLRARVREHFGAGARKGRDAELAARVKRVEWIETAGELDTSLRENA
                     LLRALAPPYNRPQEPAEAAFALRLLPNRRRAPMYETVAIAGTDPADWHGLHGVFRNRR
                     EADNLLRELAMLYRLCPRRLGLEGGNGACTAYASRRCAGVCARRETPEEHDARLAGAL
                     AGVGLKSWPWAGPVVVEEARPHGPRRLQVFDR"
     gene            complement(1384..1572)
                     /locus_tag="PKV97_15980"
     CDS             complement(1384..1572)
                     /locus_tag="PKV97_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HNB07432.1"
                     /translation="MAMYESDHTKFMREWMEKHPEQRDEQKEGRALWWDKPQSVEEQK
                     RVAAARVPVKPYYYDINN"
     gene            complement(1660..>2516)
                     /locus_tag="PKV97_15985"
     CDS             complement(1660..>2516)
                     /locus_tag="PKV97_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
                     /protein_id="HNB07433.1"
                     /translation="MPLRSELRNGDQVEIITAAHANPNPAWLSYARTGKARAQIRHFM
                     KNAQQDESVTLGERLLNQALRPHGLTLGQISSFAWDRFLRDRGVRHKKEVFSDLGLGK
                     RLPVIVARRLAEAQDLESGRPDVVKPKPAGAISIRGSEGVAVQLARCCHPIPGDPIIG
                     TLRKGQGLEVHTHDCPNVAKLRGDRGRWVDVEWEHMVDDRLFDVGIRVLSHNACGVLA
                     RVASAIAEEDSNIQSVSMDGGQGEYTALNFTVQVHDRMHLARVMRGVRRVQEVVRIGR
                     VKADGRVG"
BASE COUNT          340 a          894 c          920 g          362 t
ORIGIN      
        1 cggtcgaaca cctgcaggcg acgcggaccg tgcgggcgcg cctcttccac gaccaccggc
       61 cccgcccagg gccaggactt gaggcccaca ccggcgagcg caccggcgag acgggcgtcg
      121 tgctcctcgg gcgtctcacg gcgcgcgcac acgcccgcgc agcgccgcga ggcgtaggcg
      181 gtacaggcgc cgttgccgcc ctccaggcca agccggcgcg ggcacagccg gtacagcatc
      241 gccagctcgc gcagcaggtt gtcggcctcg cggcgattgc ggaagacgcc gtggagccca
      301 tgccaatccg ccgggtcggt gccggcgatc gccaccgtct cgtacatcgg cgcgcgccgc
      361 cggttgggca gcaggcgcag cgcgaaggca gcctcggcgg gctcctgcgg gcggttgtag
      421 ggcggagcca gcgcacgcag caaggcgttc tcgcgcagcg aggtgtcgag ttcgccggcg
      481 gtctcgatcc actccacccg cttcacccgc gccgccagct cggcgtcgcg ccccttgcgc
      541 gcacccgcgc cgaaatgctc gcgcacccgc gcgcgcagac tctgcgcgcg cccgacgaag
      601 agtgccgggt cgcgcgcgcc cgcaggcggt ggctcgggct gggcgtggaa aagatagagc
      661 ccgggggcgt cgggcagggc ctcgagcatg ccttcgggca agccgggcgg ctgcgtcggc
      721 gccttcatcg cgcgtgcggc tgcgcgcgcc agcacctcgg gcgcgaagcc ggcctccagc
      781 ccgcgcacga actgccacaa cacctcgacg tcgcccatcg cgcggtggcg cgcggcgcag
      841 acgaggccgt ggcgctcgat cagcgcatcc agtccatggc ggtggtgttc ggggtagagg
      901 gcgcgcgaca gcttgacggt gcacagcacc ggcgcctcga actcgcgacc gatgcgggtg
      961 aaggcgttgc ggatgaagcc gtagtcgaag cgcgcgctgt gggcgacgaa gacgcagccc
     1021 tcgagcagcg cggcgaccgt atcggccaac acctcgaagg cgggcgcctc ggccaccatg
     1081 gcgtcggaga tgccggtgac ctcctcgatc agcggcggga tgggctggcc gggcgcgacc
     1141 agactctccc agcgcgcgac gacctcgccg tcctcgacgc gcaggatggc gatctcggtg
     1201 atgcggtcgc gcaccgggtg cgcgccggtg ctctcgacat cgatgaaggc gagacgactc
     1261 ggcaggctca tgtcggcctg acgcgatgcc ggaggcgagc ttgcaacgga gggacgaggc
     1321 gccgcggccg gagcgggcgg cggcgcgagc acagccgcgt cacccccggc gcgtcatgcc
     1381 ggatcagttg ttgatgtcgt agtagtaggg cttgaccggc acgcgggcgg cggcgacgcg
     1441 cttctgctcc tcgacgctct gcggcttgtc ccaccacagg gcacggccct ctttctgctc
     1501 gtcgcgctgc tccgggtgct tctccatcca ctcgcgcatg aacttggtgt gatcggattc
     1561 gtacatcgcc atgtcgggtc ccccgtgggt tcacgtgcgg cgccacagag gcgtcgcggt
     1621 cggcgcgcat tctagcagcg tcggcggacg gcggggtcct cagccgacgc ggccgtcggc
     1681 cttgacgcgt ccgatgcgca ccacttcctg caccctgcgc acgccgcgca tcacgcgcgc
     1741 caggtgcatg cggtcgtgca cctgcacggt gaagttgagc gcggtgtatt cgccctgtcc
     1801 gccatccatg ctgacgctct ggatgttgct gtcctcctcg gcgatcgcac tcgccacccg
     1861 cgccagcacg ccgcaggcgt tgtggctgag cacgcggatg ccgacgtcga acaggcggtc
     1921 gtcgaccatg tgctcccact cgacatccac ccagcgcccg cggtcgccgc gcagcttggc
     1981 cacgttgggg cagtcgtggg tgtggacttc gaggccctgg cccttgcgca gggtgccgat
     2041 gatcgggtcc cccgggatgg gatggcagca gcgcgcgagc tgcaccgcca caccttccga
     2101 gccgcggatg ctgatcgccc ccgccggctt gggcttgacc acgtccgggc gaccggactc
     2161 gaggtcctgc gcctcggcga ggcggcgcgc gacgatgacc ggcaggcgct tgccgagccc
     2221 gaggtcggag aagacctcct tcttgtggcg cacgccgcgg tcgcgcagga agcggtccca
     2281 ggcgaagctg gagatctgcc cgagggtgag cccgtgcggg cgcagcgcct ggttgagcag
     2341 gcgctcgccg agcgtgaccg actcgtcctg ctgcgcgttc ttcatgaagt ggcggatctg
     2401 cgcgcgcgcc ttgccggtgc gcgcgtagga cagccaggcc ggattggggt tggcgtgggc
     2461 cgcggtgatg atctcgacct ggtcgccatt gcgcagctcg ctgcgcagcg gcatca
//
LOCUS       DAOQNN010000452         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_32333_length_2505_cov_7.339592, whole genome shotgun sequence.
ACCESSION   DAOQNN010000452 DAOQNN010000000
VERSION     DAOQNN010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32333_length_2505_cov_7.339592"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKV97_15990"
     CDS             complement(<1..637)
                     /locus_tag="PKV97_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNB07434.1"
                     /translation="METRAHHVLIGLFTVLVVGAALMFALWLGKSDADRQFEVYDIVF
                     QEAVSGLSKGGTVEFNGIKIGDVVSLRLDPADARRVIARVRVDSAAPVRSDTRARLVP
                     AGITGLTMIRLTSGEDPASTPLVSKGDEVARIIAAPSPLSRLLADGEDAITNVNDLLV
                     QARELLSADNVASFGRTLSNLELATGALAAQREDLNAALREVTQASRDASTA"
     gene            complement(639..1475)
                     /locus_tag="PKV97_15995"
     CDS             complement(639..1475)
                     /locus_tag="PKV97_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB07435.1"
                     /translation="MTAGLASAASDPRDNIVEVRGVRNQFGAQVVHDQLDLDVRRGEI
                     LSVVGGSGTGKSVLLRTIVGLNRPRAGSVHVFGEDLLSLPDARRKQVERRFGVLFQKG
                     ALFSSLTVAENVALPLIEHAGMSRREAAELAALKIALAGLPALAGDKFPSDLSGGMVK
                     RAALARALALDPDILFLDEPTAGLDPIGAAAFDQLILTLRDALGLTVFIVTHDLDTIY
                     TITDRVAVLAQKKVLVNAGLDEVAATDDAWIREYFHGPRGRAAATAVAAAQAVPAASE
                     NN"
     gene            complement(1472..>2505)
                     /locus_tag="PKV97_16000"
     CDS             complement(1472..>2505)
                     /locus_tag="PKV97_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB07436.1"
                     /translation="ADALRGRIDAATRVELAELGTLDTAGARLLHGLLGGERVAALLA
                     DEGALSRERRALLKAVADAIEGREERPAADEGYALGDVLAHIGHTMLVFWRHVSALTG
                     FIGLILETALRVSVQPRRWRVTALVANLERTGLDAVPIIALLTFLVGAVVAFLGATVL
                     QNFGASIFTVDLVAYSFLREFGVLLTAIIVAGRTASSFTAQIGSMRANEEIDAIRVLG
                     LDPVELLVLPRVYALLVALPILTFVAMIAGIVGGMLVSAMTLDISPVLFLSRIEDNVG
                     LIHFWVGMAKAPIFAFLIAVIGCLEGFRVQGSAQSVGEHTTSAVVQSIFVVIVVDAIA
                     ALFCMEMGW"
BASE COUNT          394 a          881 c          899 g          331 t
ORIGIN      
        1 gcgccgtgct ggcgtcgcgg ctggcctggg tgacctcgcg caacgcggcg ttgaggtcct
       61 cgcgctgcgc ggcgagcgcg cccgtggcca gttccaggtt gctcagcgtg cgcccgaacg
      121 aggccacgtt gtcggccgag agcagctcgc gcgcctgcac cagcaggtcg ttcacgttgg
      181 tgattgcgtc ctcgccgtcg gcgagcaggc ggctcagcgg cgaaggcgcg gcgatgatgc
      241 gcgcaacctc gtcgcccttc gacaccaggg gcgtgctcgc cggatcctcg ccgctggtca
      301 ggcggatcat ggtcaagccg gtgatgccgg caggcaccag gcgggcgcgg gtgtcgctgc
      361 gcaccggcgc ggcgctgtcc acgcgcacgc gggcgatgac gcggcgcgca tcggcgggat
      421 cgaggcgcag gctgaccacg tcgccgatct tgatgccgtt gaactccacc gtgccaccct
      481 tggacaggcc ggagaccgcc tcctggaaga cgatgtcgta cacctcgaac tggcggtcgg
      541 cgtcgctctt gcccagccac agcgcgaaca tcagcgcggc gccgacgacg agcacggtga
      601 agaggccgat cagcacatga tgggcgcggg tttccatgtc agttgttctc cgatgcagcg
      661 ggcacggcct gcgcggcggc caccgcggtg gcggccgcgc ggccgcgcgg gccgtggaag
      721 tattcgcgga tccaggcgtc gtcggtggcg gcgacttcgt ccaggccggc gttgaccagc
      781 accttcttct gcgccagcac ggcgacgcgg tcggtgatgg tgtagatggt gtcgaggtcg
      841 tgggtgacga tgaacaccgt gagcccgagc gcgtcgcgca gggtgaggat gagctggtcg
      901 aaggccgcgg cgccgatcgg gtcgaggccg gcggtgggtt cgtcgaggaa gaggatgtcg
      961 gggtcgagcg cgagcgcgcg cgccagcgcc gcacgcttga ccatgccgcc cgacagatcc
     1021 gacggaaact tgtcgccggc gagcgccggc agtccggcga gcgcgatctt gagcgccgcc
     1081 agctccgccg cctcgcggcg cgacatcccg gcgtgctcga tcaacggcag cgccacgttc
     1141 tcggccacgg tcagcgacga gaacagcgcg cccttctgga acagcacgcc gaagcggcgc
     1201 tcgacctgct tgcggcgcgc gtcgggcagg ctcagcaggt cctcgccgaa cacatgcacg
     1261 ctgcccgcgc gcggccggtt gaggccgacg atggtgcgca gcagcaccga cttgccggtg
     1321 cccgagccgc ccaccacgct gaggatctcg ccgcggcgca cgtcgaggtc gagctggtcg
     1381 tgcaccacct gggcgccgaa ctggttgcgc acgccgcgca cctcgacgat gttgtcgcgc
     1441 ggatcggatg cggcgctcgc cagccccgcc gtcaccaccc catctccatg cagaacagcg
     1501 ccgcgatcgc atccaccacg atcaccacga agatcgactg caccacggcc gaagtggtgt
     1561 gctcgcccac cgactgcgcg ctgccctgca cgcggaagcc ttccaggcag ccgatcaccg
     1621 cgatcaggaa ggcgaagatc ggcgccttgg ccatgccgac ccagaagtgg atgagcccga
     1681 cgttgtcctc gatccgcgac aggaacagca ccggcgagat atccagcgtc atcgccgaca
     1741 ccagcatgcc gccgacgatg cccgcgatca tcgccacgaa ggtcaggatg ggcagcgcaa
     1801 ccagcagcgc atacacgcgc ggcagcacca gcagctcgac cggatcgagg ccgagcacgc
     1861 ggatcgcgtc gatctcctcg ttggcgcgca tcgagccgat ctgcgcggtg aaggagctcg
     1921 ccgtgcggcc ggcgacgatg atcgcggtga gcagcacgcc gaactcgcgc aggaacgagt
     1981 aggccaccag gtcgaccgtg aagatgctgg cgccgaagtt ctgcagcacc gtggcgccga
     2041 ggaaggcgac caccgcgccg accaggaagg tcagcagcgc gatgatcggc accgcgtcga
     2101 ggccggtgcg ctcgaggttg gcgaccagcg cggtcacgcg ccagcgccgc ggctgcacgc
     2161 tcacgcgcag cgcagtctcc aggatcaggc cgatgaagcc ggtgagcgcg ctcacgtggc
     2221 gccagaacac cagcatggtg tggccgatgt gggcgagcac gtcgccgagc gcgtagccct
     2281 cgtccgcggc agggcgctcc tcgcggcctt cgatcgcatc ggccacggcc ttcagcagcg
     2341 cgcgccgctc gcggctcagc gcaccctcgt cggcgagcag ggcggcgacg cgctcgccgc
     2401 cgagcaagcc gtgcagcagg cgtgcgcccg cggtgtcgag cgtgccgagc tcggccagct
     2461 cgacccgcgt cgcggcgtcg atgcggccac gcagcgcatc ggcct
//
LOCUS       DAOQNN010000453         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_32547_length_2495_cov_8.051230, whole genome shotgun sequence.
ACCESSION   DAOQNN010000453 DAOQNN010000000
VERSION     DAOQNN010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32547_length_2495_cov_8.051230"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /gene="mobA"
                     /locus_tag="PKV97_16005"
     CDS             complement(<1..613)
                     /gene="mobA"
                     /locus_tag="PKV97_16005"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HNB07437.1"
                     /translation="MPPSPAPRPADRRITGILLAGGQGSRMGGVDKGLVELAGRPMAA
                     HALERLAPQVDALLINANQNLPAWQAFGHPVFGDDIGGFAGPLAGLHAGLLRAQHPFV
                     VTAPCDSPFLPADLVERLAAALHGADAQLAVAKTFGQPHPVFCLCRRDLADHLGAFLA
                     AGGRKIDRWYGSLKVVEVAFDDEEAAFRNVNTRDELAEAARDLP"
     gene            complement(650..1477)
                     /locus_tag="PKV97_16010"
     CDS             complement(650..1477)
                     /locus_tag="PKV97_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNB07438.1"
                     /translation="MTPPHRPLLSQARRPLTIEIPAVDERGDTVPTSIAGEHPLTLYV
                     DKREIVTLMTLGAAPEALAIGWLRNQRLVRSLDEIAAVQVDWDTDAVAITTRAGMQDA
                     ERKLGSRTVTTGCGQGTVFGGLMDEIDQIRLPHDRQLRQSTLYALMDAVRHHESIYKQ
                     AGAVHGCALARATPGGCAILMFFEDVGRHNAVDAIAGQMWLDGLDGSDKIFYTTGRLT
                     SEMVIKTAQMGIPFLVSRSGLTHMGWQIAQKIGLTMIGRAQGKHYLLFSGADRFERD"
     gene            1874..2341
                     /locus_tag="PKV97_16015"
     CDS             1874..2341
                     /locus_tag="PKV97_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HNB07439.1"
                     /translation="MKEFPIAVIMERRRLNNPWVDAAWEAVGVVPAFDDAAPAPRRIV
                     DEPERSQWRCGGFALELFRDEAENYFANLTAPIPKVFVLWRMDGELARPATVSVSYGE
                     AARWLDSGEQVDGVAMPREIADWVGDFVNTHYQPEPKKKVKRNDPLGLDRRRT"
BASE COUNT          349 a          802 c          984 g          360 t
ORIGIN      
        1 cgggcaggtc gcgggcggcc tcggccagct cgtcgcgggt gttgacgttg cggaaggcgg
       61 cctcctcgtc gtcgaaggcg acctcgacca ccttcaggct gccgtaccag cggtcgatct
      121 tgcgtccgcc ggcggcgagg aaggcaccga ggtgatcggc gagatcgcgc cggcacaggc
      181 agaacaccgg gtgcggctgc ccgaaggtct tggcgacggc gagctgggcg tcggcgccgt
      241 ggagggcggc ggccaggcgc tcgaccaggt ccgcgggcag gaacggggag tcgcagggcg
      301 cggtgacgac gaagggatgc tgcgcgcgca ggagcccggc atggagcccg gcgagcgggc
      361 cggcgaagcc gccgatgtcg tcgccgaaca ccggatggcc gaaggcctgc caggccggaa
      421 ggttctggtt ggcgttgatg agcagcgcgt cgacctgggg ggcgaggcgc tcgagcgcgt
      481 gtgccgccat cggccgcccg gcgagctcga ccaggccctt gtccacaccg cccatgcggc
      541 tgccctggcc gccagcgagc aggatcccgg tgatgcggcg gtcggcgggg cggggggctg
      601 gggagggagg catggtggga gctcgtcgtg cgtgggaggg ggtggcgctt cagtcgcgct
      661 cgaagcggtc ggcgccggag aacagcaggt agtgcttgcc ctgggcgcgg ccgatcatgg
      721 tgaggccgat cttctgcgcg atctgccagc ccatgtgggt gaggcccgag cgcgagacga
      781 ggaagggaat gcccatctgc gcggtcttga tcaccatctc ggaggtgagc cggccggtgg
      841 tgtagaagat cttgtcgctg ccatcgagcc cgtcgagcca catctggccg gcgattgcgt
      901 ccaccgcgtt gtgacggccg acgtcctcga agaacatcag gatcgcgcag ccgccgggcg
      961 tggccctggc gagggcgcag ccatgcaccg cgccggcctg cttgtagatg gactcgtggt
     1021 ggcgcacggc atccatcagg gcgtagaggg tggattgccg cagctggcga tcgtgcggca
     1081 ggcggatctg gtcgatctcg tccatgagtc cgccgaacac cgtgccctgg ccgcagccgg
     1141 tcgtgaccgt gcggctgccg agcttgcgct cggcgtcctg catgccggcg cgcgtggtga
     1201 tcgcgaccgc gtcggtatcc cagtccacct gcaccgcggc gatctcgtcc aggctgcgca
     1261 ccaggcgctg gttgcgcagc cagccgatgg cgagcgcctc gggggcggcg ccgagcgtca
     1321 tcagggtgac gatctcgcgc ttgtcgacgt agagcgtgag cggatgctcg ccggcgatcg
     1381 aggtcggcac cgtgtcgccg cgttcatcga ccgcggggat ctcgatggtg agtggacggc
     1441 gggcctggct cagcagggga cggtgaggag gggtcatcgg ggcggggccg gggatgcgcg
     1501 agcaggcgat tgtagccgca agtccggcga ggcatgggcg gagtcgcggc tccaatccgg
     1561 ccgtgcggtc gctgcggtga tccgcccttg ggaccacggc ggcgcgcgcc gcctgcactc
     1621 gcggcctcgc cccgacacgg aattcgctac cgcggatcgc gactcacgtg gcgattcccg
     1681 cacgcgcggc ggtcggacat tgtgggagcg acgcaagtcg cgatttccga aggtgcggcg
     1741 gccgcttggt gggagcgacg cgagtcgcga tcgcggcgcc tgaggtccgt gccgcgggtt
     1801 tccacgctcg cgcacggcgc ggcgggcgct tatgctaaga aagacatgga ccttcctttg
     1861 cgggtgcagc aggatgaaag aattccccat cgcggtgatc atggagcgcc gccgcctgaa
     1921 caacccctgg gtcgatgccg cctgggaggc ggtgggggtc gtgccggcct tcgacgacgc
     1981 cgcgccggcg ccacggcgca tcgtcgacga gcccgagcgc agccagtggc gctgcggggg
     2041 cttcgcgctc gagctcttcc gcgacgaggc ggagaactac ttcgccaacc tcaccgcgcc
     2101 catccccaag gtcttcgtgc tgtggcgcat ggacggcgag ctcgcccggc cggcgacggt
     2161 ctcggtgagc tatggcgagg ccgcgcgctg gctggactcg ggcgagcagg tcgatggcgt
     2221 ggcgatgccg cgcgagatcg ccgactgggt gggggatttc gtgaacacgc attaccagcc
     2281 ggagccgaag aagaaggtca agcgcaacga tccgctcggg ctggaccgga gacggacgtg
     2341 agccgcggct ttctctcgcg ctggtcgcgg cgcaagctcg ccgcggtagc gcaggccgag
     2401 gctgcagcgc agccggcggg cgctccgccc gcgccgctgc gcacgcacgc agcggatccg
     2461 ctgccgctcg cgccggccac accggatgcg gcggg
//
LOCUS       DAOQNN010000454         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_32590_length_2493_cov_11.964315, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000454 DAOQNN010000000
VERSION     DAOQNN010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32590_length_2493_cov_11.964315"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1570)
                     /locus_tag="PKV97_16020"
     CDS             complement(26..1570)
                     /locus_tag="PKV97_16020"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HNB07440.1"
                     /translation="MTRFRLLRPIAATRISGALVAAGDMSATTGGGGSAYDAAAQNAA
                     YDALYDAAMGSNSATAKWVATNGNDTTGNGTSGNPYLTIGKAISVMSAGGEVIVKDGT
                     YSGTANWISTVQVTIPNGSAGAYTRIRAENRFGVRITMSAAPSGYGDCPINLGSKQYV
                     WVDGFIIESTFTASTGDGNDNTLVEYGSATHCRATRLIVKRKSCDQYGGAYSYGDGNV
                     FEDCHHFGSSRYAYQGGTGGGSSPAGSTVLRRCVSYMPFGPVFEPTASFNFYGSNDGG
                     YALCKDVLFANCYEIDSPHLPQKVGQNPEDLKWGSFYHPKSVRNVEHIGCGVINGGGE
                     YGGIRFDNYGGAGDELGSVVDCFVATFDTGACFSKASANGTIPLTNCTGTGATGGLTS
                     GTGFTQTNNLSSGITYPVQRSGSNGAEQKYAIGAFLSERGDANYKTVQTSMKLWPFPY
                     EDEIATVFAESIARVTQDVPTGTSASADPFSGTALDGNAKTFTRRVWESTGNATPSFA
                     AGAGVY"
     gene            complement(1574..1954)
                     /locus_tag="PKV97_16025"
     CDS             complement(1574..1954)
                     /locus_tag="PKV97_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07441.1"
                     /translation="MALTFDNSAPKAALDAITALLAGGDFQLRASTTPLADLLFQTSG
                     GAFGVGTSANPSVAVSTTIADDTTPIAGTIDNFRLTNAGGTAIISGSVGTSGSDLNVT
                     NNVIPSGATSVSCPGGLTLSLTLS"
     gene            complement(1938..2135)
                     /locus_tag="PKV97_16030"
     CDS             complement(1938..2135)
                     /locus_tag="PKV97_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07442.1"
                     /translation="MFTPEKVKMKVGISGTLVFKDAAGNVIGSTEILDPALPVQKIPQ
                     PEAPIEGKPSTPQENLHGTDL"
     gene            complement(2135..>2493)
                     /locus_tag="PKV97_16035"
     CDS             complement(2135..>2493)
                     /locus_tag="PKV97_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07443.1"
                     /translation="REVASAMELPVDDVVPSDEVLTQMQDAAAQAQQKQIEDAQNFER
                     EKIQFQADLDASKEQAIAAREDKTKQGDFIREIVKQAVAGAMTEAKGKQPGGTKKLKY
                     NYGEDGLISGAEVEEA"
BASE COUNT          406 a          801 c          782 g          504 t
ORIGIN      
        1 aaggtgtcgt ttgcgacggt ggtcatcaat agactccagc gccggctgcg aagctcggcg
       61 tcgcgttgcc ggtggactcc cacacgcggc gcgtgaaggt cttggcgttt ccgtcaagag
      121 cagtcccgga gaacggatct gcgcttgcgc tagtgccagt cggaacgtct tgagtcacac
      181 gggcgatcga ctcagcaaac acagttgcaa tctcgtcctc gtaggggaac ggccacagct
      241 tcatgctggt ctgcacagtc ttgtagttcg catcgccgcg ttcgctcagg aatgcgccga
      301 tggcatactt ctgctccgct ccattgctgc cagagcgctg caccgggtac gtgatcccgc
      361 tggacaggtt gttggtctgc gtgaaacccg tcccagaggt gagcccccct gtggcacccg
      421 tccccgtgca gttggtcagc ggaatagttc cgttggcgct tgccttggag aagcacgccc
      481 cagtatcgaa agtggcaacg aagcaatcaa cgacgcttcc aagctcgtcc ccagcgccgc
      541 cgtagttgtc gaaacggata cctccatact cgccaccgcc attgatgacg ccgcagccga
      601 tgtgctcgac gttgcgcacg ctctttgggt ggtagaacga gccccacttc aagtcttcag
      661 ggttctgccc gaccttttgc ggaaggtgcg gagagtcaat ctcgtagcag ttggcaaaca
      721 gcacatcctt gcatagcgca tacccgccat cgttcgagcc gtagaagttg aacgacgccg
      781 tgggctcaaa caccgggccg aacggcatgt aagagacgca tcggcgcagc acggttgatc
      841 cggccggaga cgagccgccg cccgtgcctc cctggtaggc gtagcgcgag ctgccgaagt
      901 ggtggcagtc ctcgaagacg ttgccgtccc catacgaata ggcgccgcca tactggtcgc
      961 agctcttgcg cttgacgatc aggcgcgtgg ctctacagtg cgtcgccgag ccgtactcga
     1021 ccagggtgtt gtcgttgccg tcgcccgtgc ttgccgtgaa cgtggactcg atgatgaagc
     1081 cgtccaccca cacgtactgc ttggagccga gattgatcgg acagtcgccg tacccagatg
     1141 gcgcggcgct catcgtgatg cgaaccccga atcggttttc cgcgcggatg cgcgtgtagg
     1201 ccccggccga gccgttcggg atcgtcacct gcaccgtgct gatccagttc gccgtgccgc
     1261 tgtaggtgcc gtccttgacg atcacctcgc cgccggccga catcaccgaa atcgccttgc
     1321 cgatggtcag gtacgggttg ccgctcgtgc cgttgccggt ggtgtcgttg ccgttcgtgg
     1381 caacccactt ggccgtagcg ctgtttgacc ccattgccgc gtcatacagc gcgtcataag
     1441 cagcgttctg cgcggctgca tcgtatgcag agccaccgcc cccggttgtt gccgacatat
     1501 cacccgccgc aacgagcgca ccggaaatgc gcgtcgctgc gatcgggcgc aataggcgga
     1561 accgggtcat ggatcacgac agcgtgaggc tcagcgtcag gccaccgggg caggataccg
     1621 atgtggcgcc ggaaggaatc acgttgttgg tgacgttcag gtctgaaccc gacgtaccca
     1681 ccgaaccgga gatgatcgcc gtgcccccgg cgttggtcag ccggaagttg tcgatcgtgc
     1741 cggcgattgg cgtggtgtcg tccgcgatgg tcgtcgacac ggccacggaa gggttcgccg
     1801 aggtgccgac gccgaacgcg ccgcccgagg tctggaacag cagatccgcc agcggcgtcg
     1861 tgctggcgcg cagctggaag tcgccgccgg ccagaagcgc cgtgatggcg tcgagcgccg
     1921 ccttcggcgc ggaattgtca aaggtcagtg ccatggaggt tctcctgggg cgtggaaggc
     1981 ttgccctcga tgggtgcctc gggttgtgga atcttctgca ccggcagcgc cggatcgagg
     2041 atttccgtcg acccgatcac gttgccggca gcgtccttga agaccagcgt gccactgatc
     2101 cccactttca tcttgacctt ctcgggggtg aacatcacgc ttcctccact tcggcgccgg
     2161 agatcagccc gtcctcgccg tagttgtact tgagcttctt cgtgccgcca ggctgcttgc
     2221 ccttggcttc ggtcatcgcg ccggccacgg cctgcttgac gatctcgcgg atgaagtcgc
     2281 cctgcttggt cttgtcctcg cgcgccgcga tagcctgttc cttgctggcg tcgagatcgg
     2341 cctggaattg gatcttctcg cgttcgaagt tctgcgcgtc ctcgatctgc ttctgctgcg
     2401 cttgcgcggc cgcgtcctgc atctgggtca gcacctcgtc cgagggcacc acgtcgtcga
     2461 ccggcagttc catcgcgctg gccacctcgc gca
//
LOCUS       DAOQNN010000455         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_32876_length_2480_cov_7.653608, whole genome shotgun sequence.
ACCESSION   DAOQNN010000455 DAOQNN010000000
VERSION     DAOQNN010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32876_length_2480_cov_7.653608"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PKV97_16040"
     CDS             complement(<1..615)
                     /locus_tag="PKV97_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB07444.1"
                     /translation="MTDPAPFMLSPFGLPPLSVLFLAFLVAGTVLGLGLLHRHAHHVR
                     RHRDAVPQPFAGSIPLHSHQRAADYTVARARLSAFHAAANAGFVLALTLGGGLQAMHD
                     AWADVLPAGGLAHGVALLASLGVLGWLFELPFALLRTFGIERTFGFNRMTPRLYLADT
                     VREAALAALIGLPLLAAVLWLTLATGALWWAWVWAFWLGFNLLAM"
     gene            801..1349
                     /gene="orn"
                     /locus_tag="PKV97_16045"
     CDS             801..1349
                     /gene="orn"
                     /locus_tag="PKV97_16045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNB07445.1"
                     /translation="MAQDPKTLIWLDMEMTGLEPDRDRIIEIAIVLTDEALEVIAEAP
                     VIAVHQPDSVLDGMDEWNRNTHGKSGLTARVRASTMSEADAEAQVLAFLRAHVPARTS
                     PMCGNSVCQDRRFLARWMPELEAWFHYRNLDVSTLKELARRWRPEIYAGVKKAGSHTA
                     LADIHESIAELRHYRDHFLRLD"
     gene            1366..1527
                     /locus_tag="PKV97_16050"
     CDS             1366..1527
                     /locus_tag="PKV97_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07446.1"
                     /translation="MRIGPAGRVRGLVAWASRHRRWLIVLALLSVFFSVLGYFTNPVS
                     LPGMCRIYG"
     gene            complement(1774..>2480)
                     /locus_tag="PKV97_16055"
     CDS             complement(1774..>2480)
                     /locus_tag="PKV97_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07447.1"
                     /translation="LSQARLIPLVPALALLAAGGVASLRRGAASALDWFSLMSLAVFG
                     ILVWLAWSAQVAGWPSGLARHVARNAPGFVPPDANLRLLAGGAISVVWILLVARLPRS
                     PARAPANWAIGLVMLWSLAVVLLMPWFDQGRSYRTVVESLDIAVRGERGAQARECIAT
                     TGLPDAMKLSMDYFAGLRARPIDADATPCALLLVHVERRGRSAALAPEWETVWEYRRG
                     AGKRAETFRLLRRAGR"
BASE COUNT          419 a          822 c          926 g          313 t
ORIGIN      
        1 catcgcgagc aggttgaagc cgagccagaa cgcccacacc caggcccacc acagcgcgcc
       61 cgtcgccagc gtcagccaca gcaccgcggc gagcagcggc agcccgatca gcgcggcgag
      121 cgcggcctcg cgcacggtgt cggcgaggta gaggcgcggc gtcatgcggt tgaagccgaa
      181 ggtcctctcg atgccgaagg tgcgcagcag cgcgaagggt agttcgaaca gccagccgag
      241 cacgcccagg ctggcgagca gcgcgacgcc gtgggcgagc ccgccggcgg gcagcacgtc
      301 cgcccaggcg tcgtgcatcg cctgcagccc gccgccgagc gtgagcgcga gcacgaagcc
      361 ggcgttggcc gcggcgtgga aggcggacag gcgcgcgcgc gcgacggtgt agtcggcggc
      421 acgctggtgc gaatgcaggg gaatcgagcc ggcgaagggc tgcggcaccg cgtcgcgatg
      481 gcggcgcacg tggtgggcgt ggcgatgcag cagaccgagg ccgagcacgg taccggcgac
      541 caggaaggcc aggaacagga cggaaagagg cggcaggccg aagggcgaca gcatgaatgg
      601 ggcgggatcg gtcattgcga agcgcgggcg gtggatggag gtagggctgc gggcaggtgt
      661 gcgacccgac cggccatgcg ctgctgtccg tgacaggctg gcgcgatcgc tgctggccga
      721 ggccggcaac gtacgcgatc tatgacacaa tcgcgccctc attgtttcgc aggaaggccc
      781 cggaaaaggg cggacggatt atggcacagg acccgaagac gctgatatgg ctggacatgg
      841 agatgaccgg cctcgaaccc gatcgtgacc gcatcatcga gatcgcgatc gtgctcaccg
      901 acgaggcgct cgaagtgatc gccgaggcgc cggtgatcgc cgtgcatcag cccgacagcg
      961 tgctcgacgg catggacgag tggaaccgca atacccatgg caagtccggg ctcaccgctc
     1021 gggtacgcgc ctccacgatg tcggaggccg atgccgaggc gcaggtgctg gccttcctgc
     1081 gcgcgcacgt gcccgcgcgc acctcgccga tgtgcggcaa ttcggtgtgc caggaccgca
     1141 ggttcctcgc gcgctggatg ccggaactcg aggcctggtt ccactaccgc aacctcgacg
     1201 tgtccacgct caaggagctc gcgcggcgct ggcgtccgga gatctacgcg ggtgtcaaga
     1261 aggcgggcag ccacaccgcg ctcgccgaca tccacgagtc gatcgccgag ctgcgccact
     1321 atcgcgacca cttcctgcgc ctggactgag cgcgaggagc gcgacatgag gatcggtccc
     1381 gccgggcggg tccgcggcct cgtcgcctgg gcgagccgtc accggcgctg gttgatcgtg
     1441 ctcgcgctgc tgtcggtgtt cttcagcgtg ctgggctatt tcaccaaccc ggtatcgctg
     1501 ccggggatgt gcaggatcta cggctgaggg ccgtgcgcag gcgctggccc tcgacgtccg
     1561 tcgcagccga accgtccgat cgcgacttgc gtcgctccca cgaaaaccga cagggtgccc
     1621 ccggggtgac gcggcgccaa ggcgttacgg gttccggaag gtgacgggta ccggcgcgtg
     1681 aagcggcttc gacgtcgggt aggagcgacg caagtcgcga ttcgggcagg cgagaatgca
     1741 aggacctcgc cgcaacggca cggatgcgtc gattcagcgt ccggcgcggc gcagcagccg
     1801 gaaggtttcg gcgcgcttgc cggcaccacg gcggtattcc cacacggttt cccattcggg
     1861 tgcgagcgcg gcggagcggc cgcggcgctc gacatggacc agcagcagag cacacggtgt
     1921 ggcgtcggcg tcgatcgggc gtgcgcgcag gccggcgaaa tagtccatcg acagcttcat
     1981 cgcgtcgggc agcccggtgg tggcgatgca ttccctcgcc tgtgcgccgc gctcgccgcg
     2041 caccgcgata tcgagcgatt ccaccaccgt gcggtagctg cgtccctggt cgaaccaggg
     2101 catcagcaac accaccgcca ggctccacag catcaccagg ccgatcgccc agttcgccgg
     2161 cgcacgtgcg ggcgaccggg gcaggcgggc gacgaggagg atccacacca cgctgatggc
     2221 gccgccggca agcaggcgca ggttggcgtc cggcggcacg aagccggggg cgttgcgcgc
     2281 gacgtggcgt gcgagaccgc tcggccagcc ggcgacctgg gcgctccatg ccagccagac
     2341 gaggatgccg aagacggcca ggctcatcag cgagaaccag tcgagcgcgc tcgcggcgcc
     2401 gcggcgcagg ctggccacgc cgccggcggc gagcagggcg agcgcgggca ccagcgggat
     2461 cagccgggcc tgcgagagat
//
LOCUS       DAOQNN010000456         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_32916_length_2478_cov_11.337598, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000456 DAOQNN010000000
VERSION     DAOQNN010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32916_length_2478_cov_11.337598"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PKV97_16060"
     CDS             <1..1373
                     /locus_tag="PKV97_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB07448.1"
                     /translation="EEASGVAPPSATAPAATPDRDLRRSYKPITPPEPRRGSDASRDS
                     GDADATARVAGAEASTPIPGTGIATRVAPTSRPADRLARFSLARLASFSAREATELRR
                     DPVRLTLALLGSLVLMLVMGYGITMDVEDLHYAVLDHDRSVASQSYTLRIAGSRYFLE
                     QAPLEGHADLDARMRAGKLAMAIEIPPGFGRDLARGHAPQVAVWLDGSMPLRAELARA
                     YVGGVHAQTLADVIAESGAGPVRALASVEPRYRYNPELLSLVSMVPKVIPLLLVFIPA
                     MLTALGVVREKELGSILNVYTTPVTRLEFLLGKQLPYIALSMFNFALLFVLAVTLFQV
                     PFKGSFLALATGALLYVTATTGLGLLASTLTNSQIAALAGTSIGTLLPAIQFSGLMDP
                     VSALEGGGYWIGAIYPTTHFLTIASGTFSKALGFADLAGAFVPLALAIPVLTLLSALL
                     LKKQAK"
     gene            1385..>2478
                     /locus_tag="PKV97_16065"
     CDS             1385..>2478
                     /locus_tag="PKV97_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB07449.1"
                     /translation="MVRHPTPPKQPDQAGRSTHLRNTWNLGLKEFRSLSRDLAMVFLI
                     VFMFSASIYADARAKPESLSRAAIAVVDEDGSQLSGRIVAALQPPQFLPPEHVGLADM
                     DRGLDAGRHTFVLDIPPNFQRDVLAGRSPALQLNIDATRQGQAQTGAAYIQNILADEV
                     RAFVQRMRAPAPQPVELVPRAMFNPNLEASWFAAINAIINNVTMLGILLTGAALIRER
                     EHGTIEHLLVMPVTPLEIMLSKIWSMGVVVLVASSFSLQVMVKGVLGIDVAGSALLFG
                     AGTLLYLFAAASIGIYMGTIARSMPQFGLMSILILLPLQILSGGVTPRESMPEAIQTI
                     MGLAPTTHYVSLAQSILFRGAGLDVVWPAFA"
BASE COUNT          347 a          977 c          766 g          388 t
ORIGIN      
        1 tggaagaggc gagcggcgtc gcaccgccct ccgcgacggc gcctgctgca acgcccgatc
       61 gcgacttgcg tcgctcctac aaacccatca cacccccgga gccgcgacgt gggagcgacg
      121 caagtcgcga ttcaggcgat gccgacgcga cggcgcgcgt tgcaggcgcc gaagcgagca
      181 cgccgattcc gggcaccgga atcgcgactc gcgtcgctcc cacatcccgg cccgccgatc
      241 gcctcgcccg cttcagcctc gcccgcctgg cgagcttctc ggcgcgcgag gccaccgagc
      301 tgcgccgcga tccggtgcgg ctcacgctgg cgctgctggg cagcctggtg ctgatgctgg
      361 tgatgggcta cggcatcacc atggacgtcg aggacctgca ctacgcggtg ctcgaccacg
      421 accgcagcgt cgccagccag agctacaccc tgcgcatcgc cggctcgcgc tatttcctcg
      481 aacaggcgcc gctcgagggc cacgccgacc tcgacgcgcg catgcgtgcc ggcaagctgg
      541 cgatggcgat cgagatcccg cccggcttcg gccgcgacct cgcgcgcggc cacgcgccgc
      601 aggtggcggt gtggctggac ggctcgatgc cgctgcgcgc cgagcttgcg cgcgcctacg
      661 tcggcggcgt gcacgcacag accctcgccg acgtgatcgc cgaatccggc gccggcccgg
      721 tgcgcgcgct cgccagcgtc gagccgcgct accgctacaa ccccgagctg ctcagcctgg
      781 tgtcgatggt gcccaaggtg atcccgctgc tgctggtctt catccccgcc atgctcaccg
      841 cgctcggcgt ggtgcgcgag aaggagctcg gctccatcct caacgtctac accaccccgg
      901 tgacccggct cgagttcctg ctcggcaagc agctgcccta catcgcgctg tcgatgttca
      961 acttcgcgct gctcttcgtg ctcgcggtca cgctcttcca ggtgccgttc aagggcagct
     1021 tcctcgcgct cgccaccggt gcgctgctct acgtcaccgc caccaccggc ctgggcctgc
     1081 tcgcctccac gctcaccaac agccagatcg ccgcactcgc cggcacctcg atcggcaccc
     1141 tgctgccggc gatccagttc tccgggctga tggacccggt gagcgcgctc gaaggcggcg
     1201 gctactggat cggcgcgatc taccccacca cccacttcct caccatcgcc agcggcacct
     1261 tctccaaggc gctcggcttc gccgacctcg ccggcgcctt cgtgccgctc gcgctcgcga
     1321 tccccgtgct cacgctgctg tcggcgctgc tgctgaagaa gcaggccaag tgagacccgc
     1381 caccatggtc cgccacccga ctccaccgaa gcagccggac caagccggcc gcagcaccca
     1441 cctgcgcaac acctggaacc tcggcctcaa ggagttccgc agcctgtcgc gcgacctcgc
     1501 gatggtcttc ctgatcgtgt tcatgttcag cgcctcgatc tacgccgacg cgcgcgccaa
     1561 gccggagagc ctgtcgcgcg ccgcgatcgc ggtggtggac gaggacggct cgcagctctc
     1621 cggccgcatc gtcgccgcac tgcagccacc gcagttcctg ccgcccgagc acgtcggcct
     1681 cgccgacatg gaccgcggcc tcgacgccgg ccgccacacc ttcgtgctcg acatcccgcc
     1741 caacttccag cgcgacgtgc tcgccggccg cagcccggcg ctgcagctca acatcgacgc
     1801 cacccgccag ggccaggcgc agaccggcgc ggcctacatc cagaacatcc tcgccgacga
     1861 ggtgcgcgcc ttcgtgcagc gcatgcgcgc cccggcgccg cagccggtcg agctcgtgcc
     1921 gcgcgcgatg ttcaacccca acctcgaggc gagctggttc gccgcgatca acgcgatcat
     1981 caacaacgtc accatgctcg gcatcctgct caccggcgcc gcactgatcc gcgagcgcga
     2041 gcacggcacc atcgagcacc tgctggtgat gccggtcacc cccctcgaga tcatgctgtc
     2101 gaagatctgg tcgatgggcg tggtggtgct ggtggcctcg tccttctcgc tgcaggtgat
     2161 ggtgaagggc gtgctcggca tcgacgtggc cgggtcggcg ctgctcttcg gcgccggcac
     2221 cctgctctac ctcttcgccg ccgcctcgat cggcatctac atgggcacga tcgcacgctc
     2281 gatgccgcag ttcgggctga tgtccatcct gatcctgctg ccgctgcaga tcctctccgg
     2341 cggcgtgacc ccgcgcgaga gcatgcccga ggccatccag accatcatgg ggctggcgcc
     2401 gaccacgcac tacgtgtcgc tcgcgcagtc catcctcttc cgcggcgccg ggctggacgt
     2461 ggtgtggccg gccttcgc
//
LOCUS       DAOQNN010000457         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_32944_length_2477_cov_12.472337, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000457 DAOQNN010000000
VERSION     DAOQNN010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32944_length_2477_cov_12.472337"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..453)
                     /locus_tag="PKV97_16070"
     CDS             complement(172..453)
                     /locus_tag="PKV97_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07450.1"
                     /translation="MILDPVSSVLTDPRFPTLAVWISATPVNEAGALVAYVICRNDRG
                     EVLFCSPAIPVPKPVTAADFYPKSIGAPKARKRGKKKSKRTKRTKRSKR"
     gene            complement(469..987)
                     /locus_tag="PKV97_16075"
     CDS             complement(469..987)
                     /locus_tag="PKV97_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07451.1"
                     /translation="MSRRLAREGIYKGRATGWAVRKLPTGSISIRVEFEVHQRMEPDG
                     SWKPGPAASVAGDFFVIKQTGQPNETVASMLCETIGWGGTFAETTETPPLDAVVQIEV
                     EGRDWKGKTYFDAKWIRQENDAGKKRSAGNEDLDAVAVVNLDKKHAADLRAIAAKAKK
                     PAATNPDDGIPF"
     gene            complement(984..1982)
                     /locus_tag="PKV97_16080"
     CDS             complement(984..1982)
                     /locus_tag="PKV97_16080"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB07452.1"
                     /translation="MSGFRKAKAEQAALKMGVYGPAGSGKTFTSLLLAEGLAAATKKR
                     VAYIDTEHGTDFYCKDVPSRAVHPQAFDFDALYTRSLTEITAAVHGIDTATHGVVVID
                     SVTHLWEAAIAAYAGKKTSIDSIPMHAWGKIKKPYKDLMAFLLSSPLHVILCGRQGTE
                     YSTDDETEELKAVGVKMKAEGETPYEPHILIRMEAQKPRKTNEVAQIVAYAEKDRTGV
                     LAGRSFVNPNFESLCAPLLPLLGDVQAQIASADEAASKDAEKLIEQERERGETSTRLL
                     REWSAKVQLANDPKALKYVGKGITPQLKSQMLPSDVAALREVYQAREAEIGKAGVA"
     gene            complement(2054..2188)
                     /locus_tag="PKV97_16085"
     CDS             complement(2054..2188)
                     /locus_tag="PKV97_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07453.1"
                     /translation="MSHPERRGPRIVLAILLAIAAVGVLLGDAYLNGLEAAYEEALSK
                     "
     gene            complement(2185..>2477)
                     /locus_tag="PKV97_16090"
     CDS             complement(2185..>2477)
                     /locus_tag="PKV97_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07454.1"
                     /translation="RAGGAGAVSTPSFPHRLLWCRLESVEFPDCNTCRLNLAPGDCTD
                     MSGAIRVARAITRHLEGFILLRIETVAVGEPDTIYILADKKSKWVAREALEP"
BASE COUNT          347 a          827 c          802 g          501 t
ORIGIN      
        1 cgaccggggt gattcggacg cggaccaact cgccaccggg gttgttgcgc gcccagcgag
       61 ccgtggtttc acgatccgcg cagcaccacc ccggcttgat cttgccgtcg tagagtgcgc
      121 accacgccag ggccggcttc ggcagccgct tcttcgcctt cttcttcgcg ctcatcgctt
      181 gctcctcttg gtgcgcttgg ttcgcttgga cttcttcttc ccgcgctttc tagccttggg
      241 agcgccaata ctcttggggt agaagtcggc cgcggtcacc ggcttaggaa ccgggattgc
      301 cggcgagcag aacaggactt cgccccggtc gtttcggcag atgacgtagg cgaccagtgc
      361 gcccgcctcg ttcaccggag ttgcagagat ccacacggca agcgttggga atcgcgggtc
      421 ggtcagcacc gacgaaaccg ggtcgaggat catcacatcg cctccttgtt agaacggaat
      481 gccgtcgtcg gggttggtgg ccgctggctt cttcgccttg gccgcaatcg cacgcagatc
      541 ggccgcgtgc ttcttgtcga ggttgaccac cgccacggcg tccaagtcct cgttgccggc
      601 gctgcgcttc ttgccggcgt cgttctcctg cctgatccac ttcgcgtcga agtaggtctt
      661 gcccttccag tcgcggccct cgacttcgat ctgcaccacc gcatccagcg gcggcgtctc
      721 ggtggtctcg gcgaacgtgc cgccccagcc gatggtctcg cacagcatcg aggccaccgt
      781 ctcgttgggc tgccccgtct gcttgatgac gaagaagtcc ccagccacgg acgcggccgg
      841 gccaggcttc cacgagccgt cgggctccat gcgctggtgc acctcgaact caacgcggat
      901 ggagatggac ccggtaggca gcttgcgcac agcccacccg gtcgcccgcc ccttgtagat
      961 gccctcgcgg gcaagtcggc ggctcatgcg acacctgcct tgccgatctc ggcctcgcgg
     1021 gcttggtaga cctcacgcag cgccgccacg tcgctcggga gcatctgcga cttgagctgc
     1081 ggcgtgatgc ccttgcccac gtacttgagc gccttcgggt cgttggcgag ctgcaccttc
     1141 gcactccact cgcgcagcag acgcgttgag gtctccccgc gctcgcgttc ctgctcgatc
     1201 agcttctcgg cgtccttcga tgcggcctcg tcggccgatg caatctgtgc ctgcacgtcg
     1261 ccaagcaacg gcagcagcgg ggcgcacagg ctctcgaagt tcgggttgac gaagctgcgc
     1321 ccagccagca cgccggtccg gtccttctcg gcataggcga cgatctgcgc cacctcgttg
     1381 gtctttcgag gcttctgtgc ctccatgcgg atgaggatgt gcggctcgta gggagtttcg
     1441 ccctcggcct tcatcttcac tcccaccgcc ttgagttctt cggtctcgtc gtcggtggag
     1501 tactccgtgc cctgccgtcc gcacaggatg acgtgcagcg ggctcgacag caggaacgcc
     1561 atcagatcct tgtatggctt cttgatcttg ccccatgcgt gcatggggat gctgtcgatg
     1621 ctggtcttct tgccggcgta ggcggcgatc gcggcttccc acaggtgcgt cacgctgtcg
     1681 atgacgacga cgccatgggt tgcggtgtcg atgccgtgga cggcggccgt gatctcggtg
     1741 aggctgcgcg tgtagagcgc gtcgaagtcg aacgcctgcg ggtggacggc gcggctgggc
     1801 acgtccttgc agtagaagtc ggtgccgtgc tcggtgtcga tgtaggcgac gcgcttcttc
     1861 gtcgcggcgg ccagcccctc ggcaagcaga aggctggtga aggtcttgcc gcttccggcg
     1921 gggccataga cccccatctt gagtgcggcc tgttcggcct tggctttgcg gaatccgctc
     1981 atggtagcaa tctccgttgg gatgtagctg gggctgggat gccgcgcgct cgccccatgg
     2041 cgcgcggtga atgtcacttg ctgagagctt cctcgtaggc cgcttccagc ccgttcaggt
     2101 aggcatcgcc cagcagcacg ccaacagcgg cgatggcgag caggatggcg agcacgatgc
     2161 gcggcccgcg gcgttcgggg tggctcacgg ctccagcgcc tcccgcgcga cccacttcga
     2221 cttcttgtcg gcgaggatgt agatggtgtc gggctctccc acggcgaccg tctcgatcct
     2281 caggaggatg aacccttcaa gatgccgcgt gatagccctt gcaaccctga tggctcccga
     2341 catgtccgtg cagtcgccag gggcaaggtt gagccggcag gtgttgcagt cggggaactc
     2401 gaccgactcc agcctgcacc acagcagccg gtgagggaac gatggcgtgc tcacggctcc
     2461 agcgcctccc gcgcggc
//
LOCUS       DAOQNN010000458         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_33031_length_2473_cov_10.236146, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000458 DAOQNN010000000
VERSION     DAOQNN010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33031_length_2473_cov_10.236146"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2473
                     /gene="rpoB"
                     /locus_tag="PKV97_16095"
     CDS             <1..>2473
                     /gene="rpoB"
                     /locus_tag="PKV97_16095"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNB07455.1"
                     /translation="EQEVYMGEIPLMTTTGSFVINGTERVIVSQLHRSPGVFFEHDRG
                     KTHSSGKLLFSARIIPYRGSWLDFEFDPKDYLYFRVDRRRKMPVTILLRAIGMTPEEI
                     LATFHDTDTFHLQGEGAQFELVPERLRGEVARFDITTPDGKIIVAREKRITSKHIREI
                     DQAGVKTMAVPDDFLLGRVIATNVVDPATGELLARANDEITEDLLDKLRLAGVTGLST
                     LYINDLDRGGYISQTLRIDETADQWAAKVAIYRMMRPGEPPTEDAVEGLFQGLFYAEE
                     RYDLSSVGRMKFNRRAYPEKIDDKAPGWLKRFYQRVGAQGEDGPGVLVNDDILAVVGV
                     LVELRNGRGEIDDIDHLGNRRVRSVGELAENQFRAGLVRVERAVKERLSQAESDNLMP
                     HDLINAKPISAAIKEFFGSSQLSQFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEV
                     RDVHPTHYGRVCPIETPEGPNIGLINSLAVYARTNRHGFLETPYRKVNDGKVTDQIDF
                     LSAIEEGQYVIAQANAEIDEAGMLAGDLVSCRHKGEFLLATSDMVQYMDVAPGQIVSV
                     AASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPLVGTGIERRVAVDSGTAVQATRG
                     GIVDYVDANRVVIRVNDDETLPGEVGVDIYNMIKYTRSNQNTNINQRPVVKVGDLIAR
                     GDVVADGASTDLGELALGQNMLVGFMPWNGYNFEDSILISERVVADDRFTSIHIEELT
                     VVARDTKLGPEEITRDIASLGEAQLSRLDESGIVYIGAEVEAGDVLVGKVTPKGETQL
                     TPEEKLLRAIFGEKASDVKDTSLRVP"
BASE COUNT          437 a          855 c          807 g          374 t
ORIGIN      
        1 ggagcaggag gtctacatgg gcgagatccc gctcatgacc accaccggct ccttcgtgat
       61 caacggcacc gagcgcgtca tcgtttcgca gctgcaccgt tcgccgggcg tgttcttcga
      121 gcacgaccgc ggcaagaccc actcctcggg caagctgctg ttctcggcgc gcatcattcc
      181 ctaccgcggc tcctggctgg acttcgagtt cgatcccaag gactacctgt atttccgcgt
      241 cgaccgtcgg cgcaagatgc cggtcaccat cctgctgcgc gcgatcggga tgacgccgga
      301 ggagatcctc gcgaccttcc acgacaccga caccttccac ctgcagggcg agggtgcgca
      361 gttcgagctc gttcccgagc gcctgcgcgg cgaagtcgcg cgcttcgaca tcaccacgcc
      421 cgacggcaag atcatcgtcg cgcgcgagaa gcgcatcacc tccaagcaca tccgcgagat
      481 cgaccaggcc ggcgtgaaga ccatggcggt gccggacgac ttcctgctcg gccgggtgat
      541 cgcgaccaac gtcgtcgatc cggccaccgg cgagctgctg gcgcgcgcca acgacgagat
      601 caccgaggac ctgctcgaca agctgcgcct ggccggcgtc accgggctga gcacgctgta
      661 catcaacgac ctcgatcgcg gtggctacat ctcgcagacg ctgcgcatcg acgagaccgc
      721 cgaccagtgg gcggccaagg ttgcgatcta ccgcatgatg cgtccgggcg agccgcccac
      781 cgaggatgcc gtagaggggc ttttccaggg gctgttctac gccgaggagc gctacgacct
      841 gtcctcggtc ggccgcatga agttcaaccg ccgcgcctac cccgagaaga tcgacgacaa
      901 ggcgccgggc tggctcaagc gcttctacca gcgcgtcggc gcgcagggcg aggacggccc
      961 gggcgtgctg gtcaatgacg acatcctcgc ggtggtgggc gtgctggtcg agctgcgcaa
     1021 tggccgcggc gagatcgacg acatcgacca cctcggcaac cgccgcgtgc gttcggtggg
     1081 cgagctcgcc gagaaccagt tccgtgcggg cctggtgcgc gtcgagcgcg cggtcaagga
     1141 gcgcctgtcg caggccgagt ccgacaacct gatgccgcac gacctgatca acgccaagcc
     1201 gatctcggcg gcgatcaagg agttcttcgg ctccagccag ctctcgcagt tcatggatca
     1261 gaccaacccg ctgtccgaga tcacgcacaa gcgtcgggtc tcggcactgg gcccgggcgg
     1321 cctgacgcgt gagcgcgccg gcttcgaggt gcgcgacgtg cacccgacgc actacggccg
     1381 cgtgtgcccg atcgagaccc cggaaggtcc gaacatcggc ctgatcaact cgctcgcggt
     1441 gtatgcgcgc accaaccgcc acggcttcct cgagacgccg taccgcaagg tcaatgacgg
     1501 caaggtcacc gaccagatcg acttcctgtc ggcgatcgag gaggggcagt atgtgatcgc
     1561 gcaggcgaac gccgagatcg acgaggcggg catgctcgcc ggcgacctgg tgtcgtgccg
     1621 ccacaagggc gagttcctgc tcgccacctc ggacatggtg cagtacatgg acgtggcccc
     1681 gggccagatc gtgtcggtcg cggcttcgct gattcccttc ctggagcacg acgacgccaa
     1741 ccgcgcgctg atgggcgcca acatgcagcg ccaggcggtg ccctgcctgc gcccggaaaa
     1801 gccactggtc ggcaccggca tcgagcgccg cgtggcggtc gactcgggca ctgcggtgca
     1861 ggccacccgt ggcggcatcg tggactacgt ggatgcgaac cgggtggtga tccgggtgaa
     1921 cgacgacgag accctgccgg gtgaagtcgg cgtcgacatc tacaacatga tcaagtacac
     1981 ccgttccaac cagaacacca acatcaacca gcgcccggtg gtgaaggtgg gcgacctgat
     2041 cgcccgcggc gacgtggtgg ccgacggcgc ttccaccgac ctcggcgagc tcgcgctcgg
     2101 ccagaacatg ctggtcggct tcatgccctg gaacggctac aacttcgagg actcgatcct
     2161 catctccgag cgcgtggtcg ccgacgaccg cttcacctcg atccacatcg aggaactcac
     2221 cgtcgtcgcc cgcgacacca agctcggacc cgaggagatc acccgcgaca tcgcctcgct
     2281 cggcgaggcg cagctgtcgc gcctggacga gtcgggcatc gtgtatatcg gtgccgaggt
     2341 cgaggccggc gacgtgctgg tcggcaaggt cacgcccaag ggcgagaccc agctcacccc
     2401 cgaggaaaag ctgctgcgcg cgatcttcgg cgagaaggcc tccgacgtga aggacacctc
     2461 gctgcgcgtg ccc
//
LOCUS       DAOQNN010000459         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_33032_length_2473_cov_9.264682, whole genome shotgun sequence.
ACCESSION   DAOQNN010000459 DAOQNN010000000
VERSION     DAOQNN010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33032_length_2473_cov_9.264682"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKV97_16100"
     CDS             <1..256
                     /locus_tag="PKV97_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNB07456.1"
                     /translation="NAPMQGTAADLIKKAMIAADAWLASSGLKSKLVLQVHDELVLEV
                     PKAELETMREELPRLMGGVAALKVPLLVEVGAGDNWDEAH"
     gene            575..2335
                     /gene="msbA"
                     /locus_tag="PKV97_16105"
     CDS             575..2335
                     /gene="msbA"
                     /locus_tag="PKV97_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HNB07457.1"
                     /translation="MKVYLRLLGYTRPFLGYFALSILGFAIAGACKAGLASVLKYFID
                     GLAAPDAPIATGLPWLDALDLIFAIPLMIVVIALVEGLGTFLGNYGISRVSLGLIHDL
                     RTQLFGSFLVLPNRFYDAHNSGHLVSKITYNVSMVTDAATDAVKILFREGLTVVALLG
                     YLLWMNWRLTLTLLVVLPAIAWLVSNASRKFRRLSKNIQTSMGEVTHVASETIQGYRV
                     VRSFGGEAYESARFRKASTDNTRRQLKMVRTQETFSALMHLIVYSSMGLLLFTVLKVR
                     GDASAGDVVAYITAAGLLPRAIQLLGGVSARIQRGVAAADSIFEQLDEAPEPDAGTVE
                     RERVVGRVEAQGLGFTYPGAEGKALQGIDFKAEPGQMIALVGKSGSGKSTLANLIPRF
                     YQHDEGRLLVDGVAVEDYRLANLRHHIALVTQQVTLFNGTVAQNIAYGELAGASRDQI
                     VAAAEAANAREFIDRLPQGFDTEIGENGVLLSGGQRQRLAIARALLKNAPILILDEAT
                     SALDTESERLIQAAIERLMEGRTTIVIAHRLSTIEKADQILVMEAGRIVERGTHAELV
                     ARGGVYARLQAMGIEPDAAA"
BASE COUNT          401 a          877 c          811 g          384 t
ORIGIN      
        1 caacgcgccg atgcagggca cggccgccga cctcatcaag aaggcgatga tcgcggccga
       61 cgcctggctg gcctcgtcgg ggctgaagtc gaagctggtg ctgcaggtgc atgacgagct
      121 ggtgctggaa gtgcccaagg ccgagctcga gacgatgcgc gaagagctgc ccaggctgat
      181 gggcggcgtc gccgcgctca aggtgccgct gctggtggag gtcggcgccg gcgacaactg
      241 ggacgaggcg cactgaggag gaggcgcgta atcgcgactc gcgtcgctct tacaagaagc
      301 cgagctgcaa tcgcgactcg cgtcgcgcct acaggaaacc gaaccgcttt cccgatgtgc
      361 ctgtgggagc gacgcaagtc gcgatcgcgc ggtgggcctg cgcggaggat ccccgaagcg
      421 cgataatcgc gacttgcgtc gctcctacgg aaaccggccg tggccatcgt tcctgccgcg
      481 ggcttggcat atcatccggg ccatgactgc cgtacccgaa ccgatcgcat ccagcacacc
      541 cgcccccgcc gtcgagaagc cgcccgcgag cagcatgaag gtctacctcc gcctgctcgg
      601 ctacacccgg cccttcctcg gctacttcgc gctcagcatc ctcggcttcg ccatcgcagg
      661 cgcctgcaag gcggggctgg cgagcgtgct caagtacttc atcgacggtc ttgccgcgcc
      721 cgatgcgccg atcgccaccg gcctgccctg gctggacgcg ctcgacctga tcttcgccat
      781 cccgctcatg atcgtcgtca tcgcgctggt cgaaggcctc ggcaccttcc tcggcaacta
      841 cggcatctcg cgcgtctcgc tcggcctcat ccacgacctg cgcacccaac tcttcggcag
      901 cttcctggtg ctgcccaacc gcttctacga cgcgcacaac tccggccacc tcgtctccaa
      961 gatcacctac aacgtgtcga tggtgaccga cgcggccacc gacgcggtca agatcctgtt
     1021 ccgcgagggc ctcaccgtcg tcgcgctgct cggctacctg ctgtggatga actggcgcct
     1081 caccctgacc ctgctcgtgg tgctgcccgc gatcgcctgg ctggtgtcca acgccagccg
     1141 caagttccgc cgcctgtcga agaacatcca gacctcgatg ggcgaggtca cccacgtcgc
     1201 ctccgagacc atccagggct accgcgtggt acgcagcttc ggcggcgagg cctacgaatc
     1261 cgcgcgcttc cgcaaggcga gcaccgacaa cacccggcgc cagttgaaga tggtgcgcac
     1321 gcaggagacc ttctcggcgc tgatgcacct gatcgtgtat tcgtcgatgg gcctgctgct
     1381 ctttaccgtg ctgaaggtgc gcggcgacgc ctcggccggc gacgtggtcg cctacatcac
     1441 cgccgccggc ttgctgccgc gcgcgatcca gctgctgggc ggcgtcagcg cgcgcatcca
     1501 gcgcggtgtg gccgcggccg acagcatctt cgagcagctc gacgaggcgc ccgagcccga
     1561 cgccggcacg gtcgagcgcg agcgcgtcgt cggccgcgtc gaggcgcagg ggctgggctt
     1621 cacctacccc ggcgccgaag gcaaggcgct gcaaggcatc gacttcaagg ccgaacccgg
     1681 ccagatgatc gcgctcgtcg gcaagtccgg cagcggcaaa tccacgctcg ccaacctcat
     1741 cccgcgcttc taccagcacg acgagggccg gctgctggtc gatggcgttg cggtcgagga
     1801 ctacaggctg gccaacctgc gccaccatat cgcgctggtg acgcagcagg tcacgctctt
     1861 caacggcacc gtggcgcaaa acatcgccta cggcgagctc gccggcgcca gccgcgacca
     1921 gatcgtcgcc gccgccgagg ccgccaacgc gcgcgagttc atcgaccgcc tgccgcaggg
     1981 cttcgatacc gagatcggcg agaacggcgt gctgctgtcc ggcggccagc gccagcgcct
     2041 ggcgatcgca cgcgcgctgc tgaagaatgc gccgatcctg atcctggatg aggcgacgtc
     2101 tgcgctggat accgagtcgg aacggctcat ccaggcggcg atcgagcgcc tgatggaggg
     2161 acgtacgacc atcgtcatcg cgcaccggct atcgacgatc gagaaggcgg atcagatcct
     2221 ggtgatggag gcggggcgca tcgtggagcg cgggacgcat gcggagctgg tggcccgcgg
     2281 cggcgtctac gcgcgcctgc aggcgatggg gatcgagccg gacgccgcag cttgagttgc
     2341 gatcgtgcgc gtatcgagcc gcgcagcgat cgctccgccg ccgcaatcgc gactcgcgtc
     2401 gctcctacat ctacgcccgc gccggttttc gtaggagcga cgcaagtcgc gatcgggcga
     2461 tggaccgacc gcg
//
LOCUS       DAOQNN010000460         2469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_33138_length_2469_cov_11.629246, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000460 DAOQNN010000000
VERSION     DAOQNN010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33138_length_2469_cov_11.629246"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PKV97_16110"
     CDS             complement(<1..716)
                     /locus_tag="PKV97_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNB07458.1"
                     /translation="MFDKILIANRGEIACRVIKTARRMGIKTVAVYSEADANARHVRL
                     ADEAVLIGPAAARESYLVIDKIIAAAKQTGAQAIHPGYGFLSENEDFCHACEREGIVF
                     IGPPVSAIRAMGSKSEAKKLMEAAGVPLTPGYHGDDQDPAWLHQQADAIGYPVLIKAA
                     AGGGGKGMRLVDRSEDFIDLLASCKREAISSFGDDHVLVEKYITKPRHIEIQVFGDTH
                     GNVVYLFERDCSVQRRHQKVL"
     gene            complement(760..1551)
                     /locus_tag="PKV97_16115"
     CDS             complement(760..1551)
                     /locus_tag="PKV97_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNB07459.1"
                     /translation="MSYETLEIVRAAGVATIWMNRPDVHNAFNAQLIADLTTACIELD
                     ADQAVRAVVLAGRGRSFSAGADLNWMKAAGEASEAENFADAMKLAGMLRTLAEMNKPT
                     IARVHGAALGGGMGLASACDICIAGDKAVFATSEVKFGIIPSAISPYVIRAIGERQAT
                     RYFQTAERIGAARAAELGLAHEAVASEELDAKVKEVVEALLQGGPKSQAAAKDLIRAV
                     ANRPLSDALVEDTARRIASLRATPEAKEGLAAFLDKRPAAWIAQA"
     gene            complement(1561..>2469)
                     /locus_tag="PKV97_16120"
     CDS             complement(1561..>2469)
                     /locus_tag="PKV97_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNB07460.1"
                     /translation="GGDVHTRISGVADYLAENDSHALHIARRIVSNLNQRKEVELALA
                     DSEEPAYDPEEIYGIIPADPRKPYDVREVIARVVDGSRFDEFKPRYGTTLVTGFARLY
                     GYPVGIVANNGILFGESAQKGAHFVELCGQRGIPLLFLQNITGFMVGRKYENGGIAKD
                     GAKMVTAVATVQVPKITMIMGGSFGAGNYGMCGRAYSPRFLWMWPNARVSVMGGEQAA
                     SVLSTVRRDGIEARGGTWSKEDEEAFKAPIREQYEVQGHPYYATARLWDDGVIDPAQS
                     RRVLGLSLSATLNAPIPQTKFGVFRM"
BASE COUNT          338 a          847 c          851 g          433 t
ORIGIN      
        1 agcaccttct ggtggcggcg ctgcaccgag cagtcgcgct cgaacaggta caccacgttg
       61 ccgtgcgtgt cgccgaagac ctggatctcg atgtggcgcg gcttggtaat gtacttctcg
      121 accagcacgt ggtcgtcgcc gaagctggag atcgcctcgc gcttgcagga ggcgagcagg
      181 tcgatgaagt cctccgacct gtcgaccagg cgcatgccct tgccgccgcc gccggccgca
      241 gccttgatca gcaccgggta gccgatggcg tcggcctgct ggtgcagcca ggccgggtcc
      301 tggtcgtcgc cgtggtagcc gggcgtcagc ggcacgccgg ccgcctccat cagcttcttg
      361 gcctcggact tcgagcccat ggcgcggatc gccgacaccg gcgggccgat gaagacgatg
      421 ccttcgcgct cgcaggcgtg gcagaaatcc tcgttctcgg acaggaagcc gtagccgggg
      481 tggatggcct gggcaccggt ctgcttcgcg gcagcgatga tcttgtcgat caccaggtag
      541 ctctcgcgcg ccgccgccgg gccgatcagc accgcctcgt cggccaggcg cacgtggcgg
      601 gcattggcgt cggcctcgga atacaccgcg acggtcttga tgcccatgcg gcgggcggtc
      661 ttgatgacgc ggcaggcgat ttccccgcgg ttggcaatca ggatcttgtc gaacatttcg
      721 tctccagcgt gtcttgtgtc ggttcggcgc gtcggccgct caagcctgcg cgatccacgc
      781 cgcggggcgc ttgtcgagaa aggcggccag gccttccttg gcctcgggcg tggcgcgcag
      841 gctggcgatg cggcgcgcgg tgtcttccac caacgcatcg ctcagcggcc ggttggccac
      901 cgcgcggatc aggtccttgg ccgcggcctg cgacttcgga ccgccctgca gcagggcctc
      961 gacgacctcc ttcaccttgg cgtccagttc ctcgctcgcc accgcctcgt gcgccaggcc
     1021 gagttcggcc gcgcgcgccg cgccgatgcg ctcggcggtc tggaagtagc gggtggcctg
     1081 acgctcgccg atggcgcgga tcacgtaggg gctgatcgcc gacgggatga tcccgaactt
     1141 gacctcggag gtcgcgaaca ccgccttgtc cccggcgatg cagatgtcgc aggcgctcgc
     1201 caggcccatg ccgccaccga gcgccgcgcc atgcacgcgc gcgatggtgg gcttgttcat
     1261 ctcggccagg gtgcgcagca tgccggcaag cttcatcgca tcggcgaagt tctctgcctc
     1321 gctcgcctcg ccggcagcct tcatccagtt gaggtcggcg ccagccgaga agcttcttcc
     1381 ccgcccggcc agcaccaccg cgcgcactgc ctggtcggca tccagttcga tgcaggccgt
     1441 cgtgaggtcg gcgatcagct gcgcgttgaa ggcgttgtgc acgtccgggc gattcatcca
     1501 gatcgtcgcc acgccggctg cgcgcacgat ctccagggtc tcgtagctca tctcgccccc
     1561 ttacatccgg aacacaccga acttcgtctg cgggatcggc gcgttgagcg tggccgacag
     1621 cgacaggccg agcacgcggc gcgactgcgc ggggtcgatc acgccgtcgt cccacaggcg
     1681 ggcggtggcg tagtagggat gaccctgcac ctcgtactgc tcgcggatcg gcgccttgaa
     1741 ggcttcttcg tcttccttgc tccaggtgcc gcccctggcc tcgatgccgt cgcggcgcac
     1801 cgtggagagc acgctcgcgg cctgctcacc gcccatcacg ctgacgcgcg cgttcggcca
     1861 catccacagg aagcgcggcg agtaggcgcg gccgcacatg ccgtagttgc cggcaccgaa
     1921 gctgccgccc atgatcatgg tgatcttcgg cacctgcacg gtggcgaccg cggtgaccat
     1981 cttggcgccg tccttggcga tgccgccgtt ctcgtacttg cgcccgacca tgaagccggt
     2041 gatgttctgc aggaacagca gcgggatgcc gcgctggccg cacagctcga cgaagtgcgc
     2101 gcccttctgc gccgactcgc cgaacaggat gccgttgttg gcgacgatgc ccaccgggta
     2161 gccgtagagg cgggcgaagc cggtgaccag ggtggtgccg tagcgcggct tgaactcgtc
     2221 gaaacgcgag ccgtcgacga cgcgcgcgat gacctcgcgc acgtcgtagg gcttgcgcgg
     2281 gtcggcgggg atgatgccgt agatctcctc cgggtcgtag gcgggttcct cgctgtcggc
     2341 gagcgccagt tcgacctcct tgcgctggtt gaggttggag acgatgcggc gcgcaatgtg
     2401 gagcgcgtgg ctgtcgttct cggcgaggta gtcggccacg cccgagatgc gcgtgtgcac
     2461 gtcgccgcc
//
LOCUS       DAOQNN010000461         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_33927_length_2437_cov_12.311923, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000461 DAOQNN010000000
VERSION     DAOQNN010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33927_length_2437_cov_12.311923"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PKV97_16125"
     CDS             complement(<1..627)
                     /locus_tag="PKV97_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB07461.1"
                     /translation="MTSAFTPHPEMLRIHDLHAFYGESHILHGIDIKVARGECVTLLG
                     RNGSGRSTTLKSVLGLVGKRTGSIMVNGREVIAEPTHRMARYGIGYVPEERAIFASLS
                     TEENLMLPPQVASGALSVEEIYQMFPNLQERRNSPGSKLSGGEQQMLAMARVLRTGAK
                     LLLLDEITEGLAPVIVKKLGEVIVELKSRGFTIVLVEQNFRFAAPLADR"
     gene            complement(647..1414)
                     /locus_tag="PKV97_16130"
     CDS             complement(647..1414)
                     /locus_tag="PKV97_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB07462.1"
                     /translation="MSEFILETRGLTKEFKGFVAVSNVDLKVRTGHIHALIGPNGAGK
                     TTVFNLLTKFLPPTAGSILFDGKDVTREAPAVTARRGMVRSFQISATFPNLTVLENVR
                     IGLQRRLGTEFHFWRSAKSLDVLNDEAMALLEAVDLTRFADTVTAEMPYGRKRALEIA
                     TTLALEPRMLLLDEPTQGMGGEDIARVVELIRKVSANRTILMVEHNLSVVANLCDRIT
                     VLTRGSVLAEGSYAEVSKNPKVLEAYVGSDDIGDAHH"
     gene            1750..1953
                     /locus_tag="PKV97_16135"
     CDS             1750..1953
                     /locus_tag="PKV97_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNB07463.1"
                     /translation="MANGTVKWFNDSKGFGFITPEAGGDDLFAHFSAIQGQGFKTLAE
                     GQRVTFDVTTGPKGLQASNIRAA"
     gene            2085..>2437
                     /locus_tag="PKV97_16140"
     CDS             2085..>2437
                     /locus_tag="PKV97_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07464.1"
                     /translation="MTRLIQRTELETYYYTPVHCPFCGVRVMGADPSDETRITACPHT
                     LFIAHDTGFEYRSARLDHNLHLDGLSEREIEARWHEDARGIDGFTDTVCLPDAIKVAA
                     YAPAPSAYGSYYGFAP"
BASE COUNT          411 a          804 c          782 g          440 t
ORIGIN      
        1 gcggtcggcc agcggggcgg cgaagcggaa gttctgctcc accagcacga tggtgaagcc
       61 gcgcgatttc agctcgacga tgacctcgcc gagcttcttc acgatcaccg gcgccagccc
      121 ctcggtgatc tcgtcgagca gcagcagctt ggccccggtg cgcagcacgc gcgccatcgc
      181 cagcatctgc tgctcgccgc ccgagagctt ggagcctggg ctgttgcggc gttcctgcag
      241 gttgggaaac atctggtaga tctcttccac cgacagcgcc ccgctcgcca cctgcggcgg
      301 cagcatcagg ttctcctcgg tgctgagcga ggcgaagatc gcgcgctcct cgggcacgta
      361 gccgatgccg tagcgcgcca tgcggtgggt gggctcggcg atcacctcgc ggccgttgac
      421 catgatcgag ccggtgcgct tgccgaccag gccgagcacc gatttcaagg tcgtcgagcg
      481 tccggagccg ttgcgcccga gcaaggtcac gcattcgccg cgcgccacct tgatgtcgat
      541 gccgtgcagg atgtgcgact cgccgtagaa ggcgtggagg tcgtggatcc ggagcatctc
      601 cggatgaggg gtgaaggcac tggtcatggc gtttcggggg cggggatcag tggtgggcgt
      661 cgccgatgtc gtcggagccg acgtaggcct cgagcacctt ggggttcttc gacacctcgg
      721 cgtagctgcc ttcggcgagc acgctgccgc gggtgagcac ggtgatgcgg tcgcacaggt
      781 tggcgaccac cgagaggttg tgctccacca tcaggatggt gcggttggcg gacaccttgc
      841 ggatgagttc gaccacgcgc gcgatgtcct cgccccccat gccctgggtg ggttcgtcga
      901 gcaacagcat gcgcggctcg agcgccagcg tggtggcgat ctcgagcgcg cgcttgcggc
      961 catagggcat ctcggcggtg acggtgtcgg cgaaccgggt gagatccacc gcctcgagca
     1021 gggccatcgc ctcgtcgttc agaacatcca gcgacttcgc cgagcgccag aagtggaatt
     1081 ccgttcccag cctgcgctgc aggccgatgc gcacgttctc gagcaccgtc aggttgggaa
     1141 aggtcgccga gatctggaag gagcgcacca tgccgcggcg cgcagtcacc gccggcgcct
     1201 cgcgggtgac gtccttgccg tcgaacagga tggagcctgc agtcggcggc aggaacttgg
     1261 tcagcaggtt gaagaccgtg gtcttgcccg cgccgttggg gccgatcagg gcatggatgt
     1321 gccccgtccg caccttcagg tcgacattgg atacggcgac gaagcccttg aactccttgg
     1381 tgagtccacg ggtttcgaga atgaactcgc tcaaggctgt ctcctgcccg ggttcgtttt
     1441 ttgttgcggg cagtgttccg gccgcccgga gaggcgtcaa cctatcgttt gccccaatgg
     1501 gcaaaccctg ccgggccgag cctcgacgtg cctcgggcca cgctgccggg gcaccacgga
     1561 caagaaaaat aaaatatatc tatctttatg ggcagctttc gcgcatactg cgccgcagcg
     1621 atattcaagt catgtagttc atttgtcggc atatccggtt cctcgggccg tatttcctca
     1681 agtcgtacct cgctgtcttg aacctcgccc caaccagggg gctttcccca ttttcaggag
     1741 attcaagaca tggcaaacgg caccgtgaag tggttcaacg attccaaagg cttcggcttc
     1801 atcaccccgg aagcgggtgg cgacgacctc ttcgcccact tttccgccat ccagggccag
     1861 ggtttcaaga ccctcgccga aggccagcgc gtcacgttcg acgtgaccac cggccccaag
     1921 ggcctgcagg cgtccaacat ccgcgccgcc tgagctgcat cgcgaaccgg cgcctgcggc
     1981 gccgatcgcc tcgagaggcc cgacgagagt cgggcctttt tgcttttcgg cctgccgaaa
     2041 agcggcagcg cgaccgatca cccctacgaa tggaggacag cccgatgacc cggctcatcc
     2101 agagaaccga actcgagacc tactactaca cacccgtgca ttgccccttc tgcggcgtgc
     2161 gcgtgatggg cgccgacccc agcgacgaga cgcgcatcac cgcctgcccg cataccttgt
     2221 tcatcgccca cgacaccggc ttcgaatatc ggtcggcacg cctcgaccac aatctgcatc
     2281 tcgacggcct gtcggagcgc gagatcgagg cgcggtggca cgaggacgca cgcggcatcg
     2341 acggcttcac cgacacggtg tgcctgcccg atgcgatcaa ggtcgccgcc tacgcacccg
     2401 caccctcggc gtacggctcg tactacgggt tcgcacc
//
LOCUS       DAOQNN010000462         2434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_33987_length_2434_cov_6.805380, whole genome shotgun sequence.
ACCESSION   DAOQNN010000462 DAOQNN010000000
VERSION     DAOQNN010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33987_length_2434_cov_6.805380"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PKV97_16145"
     CDS             complement(<1..772)
                     /locus_tag="PKV97_16145"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585280.1"
                     /note="catalyzes the formation of 5-methyl-uridine at
                     position 54 in all tRNAs; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HNB07465.1"
                     /translation="MPLPCIDPARYEDQLAAKLARYRSDCAEFDLPEPAVFRSAPLHY
                     RLRAEFRLWHHEGRIDYAMFDSADPKQPILLETFPAAAEPIAKLMPRLRDALQASEAL
                     RRKIFQVDFLATLSGECLVSLVYHRPLDADWETAARALAAALDIQVIGRSRKQKIVLE
                     RDWVQEVLEVDGQRLRYQQFEGSFTQPNGGVNQHMLAWARAQAKAVGARPQGPGDLLE
                     LYCGNGNFTVALAPLFGRVLATEMSKTSVRAAHTNLADN"
     gene            complement(777..1487)
                     /locus_tag="PKV97_16150"
     CDS             complement(777..1487)
                     /locus_tag="PKV97_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07466.1"
                     /translation="MEHELKTLTARLLAQRWLPRGDKLVRRALVDETFRLELDRRLAD
                     VGLRLLDNPYAAHVGVALQPELAEPVFGAGREYAASNLGLTRDEVALLVILWALIVLP
                     KRQRQVTRRELVDDGQTDLFGGDKPVAHGDAVSGTLTEASLIADFGPRLGGRTKVRNF
                     MLGKLARLGFIERRNGLVTEGPLLDLALDYRVMADRIIHGTLGEILEAAGHAPPESNA
                     FELAEVLPDEAEALDEED"
     gene            complement(1521..>2434)
                     /locus_tag="PKV97_16155"
     CDS             complement(1521..>2434)
                     /locus_tag="PKV97_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07467.1"
                     /translation="EEDAELGFLTAQLAGLQAVGGITAESLGHLLSKLNDLTWHFEEA
                     IASGSEFRILDARRRLSANGRWLERGTELLDKLLADPEVDFDIARIAQRIGLAQSRLA
                     RADASFQRALNKIEAQRVTLGASGISSSDVAAWLRGLDAAALATLAADACASVPELPL
                     LAGGHELLDRAESLLEGGGSAAPADTTLPPADDAATGLAPQAEDLRMLEHFTHRLGAL
                     PAPQPLHHVIAGGGFAPASYRLSLLALLADGAETAPEGGPEDGPVSSFMRLPVEVEFG
                     DTLTAVGEDEIGAMTLGEVRPRATTAA"
BASE COUNT          320 a          802 c          956 g          356 t
ORIGIN      
        1 cgttgtcggc gaggttggtg tgcgcggcgc gcaccgaggt cttgctcatc tcggtcgcga
       61 gcacgcggcc gaacagcggc gccagcgcca cggtgaagtt gccgttgccg cagtagagtt
      121 cgagcaggtc acccgggcct tgcggacgcg cgcccacggc cttcgcctgc gcgcgcgccc
      181 aggccagcat gtgctggttc acgccgccgt tgggctgggt gaagctgccc tcgaactgct
      241 ggtagcgcag acgctggccg tcgacctcga gcacctcctg tacccagtcg cgctcgagca
      301 cgatcttctg cttgcggctg cgcccgatca cctggatgtc gagcgcggcg gcgagcgcgc
      361 gcgcggcggt ttcccagtcc gcgtcgagcg gacggtggta gaccaggctg accaggcact
      421 cgccgctcag ggtggcgagg aagtcgacct ggaagatctt gcgccgcagc gcctcgctgg
      481 cctggagcgc gtcgcgcagg cgcggcatca gcttggcgat cggttctgca gccgcgggga
      541 aggtctcgag caggatgggc tgcttcgggt ccgcgctgtc gaacatcgcg tagtcgatgc
      601 ggccctcgtg atgccacagg cggaactcgg cgcgcaggcg gtagtgcagc ggcgcggagc
      661 ggaagaccgc cggctcgggc aggtcgaact cggcgcagtc gctgcggtag cgcgcgagct
      721 tggcggcgag ctggtcctcg tagcgcgcgg ggtcgatgca gggcaggggc atgggctcag
      781 tcttcctcgt cgagggcttc agcttcgtcg ggcaggacct cggcgagttc gaaggcgttg
      841 ctctccggcg gcgcgtggcc ggcggcttcg aggatctcgc ccagggtgcc gtggatgatg
      901 cggtcggcca tcacgcggta gtcgagcgcg aggtcgagca gtggtccctc ggtgacgagc
      961 ccgttgcggc gctcgatgaa gccgaggcgg gcgagcttgc cgagcatgaa gttgcgcacc
     1021 ttggtgcgcc ctcccaggcg tgggccgaag tcggcgatca gggaggcctc ggtgagcgtg
     1081 cccgagacgg catcgccatg cgccaccggc ttgtcgccac cgaagagatc ggtctggccg
     1141 tcgtcgacga gctcgcgccg ggtcacctgg cgctgccgct tgggcagcac gatcagcgcc
     1201 cagaggatca ccagcagcgc gacctcgtcg cgcgtcaggc cgaggttgct ggccgcgtac
     1261 tcgcgcccgg cgccgaacac cggttcggcg agttcgggct gcagcgccac gccgacgtgg
     1321 gcggcgtagg ggttgtcgag caggcgcagg ccgacgtcgg ccagccgccg gtcgagttcg
     1381 aggcggaagg tttcatccac cagcgcgcgt cgcaccagct tgtcgccgcg cggcagccag
     1441 cgctgggcga gcaggcgcgc ggtgagggtc ttgagttcgt gttccatgga aatcctgggg
     1501 gctcgggccg ggggcggggc tcaagccgcg gtggtggcgc gcgggcgcac ctcgccgagc
     1561 gtcatcgcgc cgatctcgtc ctcgccgacc gcggtcagcg tgtcgccgaa ctcgacctcc
     1621 accggcaggc gcatgaagct gctcaccggg ccatcctccg ggccgccctc cggagcggtc
     1681 tccgcgccgt cggcgagcag cgcgagcagc gacaggcggt agctcgccgg ggcgaaaccg
     1741 ccgccggcaa tcacgtggtg caggggctgc ggcgcgggca gggccccgag acgatgggtg
     1801 aagtgttcga gcatgcgcag gtcttcggcc tgtggggcga ggccggtcgc ggcgtcgtcg
     1861 gccgggggca gggtggtgtc ggccggggcc gcgctgccgc cgccctcgag caaggattcg
     1921 gcgcggtcga gcagctcgtg cccgccggcg agcagaggca gctccggcac gctcgcgcag
     1981 gcgtccgccg ccagcgtggc gagcgccgcg gcatcgagcc cgcgcagcca ggcggcgacg
     2041 tccgaggacg agatgcccga ggcccccagc gtcacgcgct gcgcctcgat cttgttcagc
     2101 gcgcgctgga aggaggcgtc ggcgcgcgcc aggcgcgact gcgccagccc gatgcgctgc
     2161 gcgatgcggg cgatgtcgaa gtcgacctcg gggtcggcga gcagcttgtc gaggagctcg
     2221 gtgccgcgtt cgagccagcg cccgttggca gacaggcggc ggcgggcgtc gaggatgcgg
     2281 aactcggagc cggaggcgat cgcctcctcg aagtgccagg tgaggtcgtt gagcttggac
     2341 agcagatggc cgagcgactc ggcggtgatg ccgccgactg cctgcaggcc ggcgagctgg
     2401 gcggtgagga agccgagctc ggcgtcttct tcct
//
LOCUS       DAOQNN010000463         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_34008_length_2433_cov_10.273339, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000463 DAOQNN010000000
VERSION     DAOQNN010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34008_length_2433_cov_10.273339"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKV97_16160"
     CDS             complement(<1..332)
                     /locus_tag="PKV97_16160"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HNB07468.1"
                     /translation="MRDATAPGTRCVQDTVRDPDYGRPVGEDCLSLNVFSPAGAAARP
                     VMVWIHGGAFANGSADIYDARRLVTRGDAVTGVEVAIVDGDDPQAVGSLTVVAPRVCV
                     SASATGTPA"
     gene            complement(280..756)
                     /locus_tag="PKV97_16165"
     CDS             complement(280..756)
                     /locus_tag="PKV97_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07469.1"
                     /translation="MPLAIAVAIALASLGTAPPAAAVALGEVAALSAMGESFRAEIRL
                     PDGKPADAECFRVVPLSGGEFPALRQGRIAVSGSGAEARLVVRDAARVNEPVLKLAIE
                     NVCEARLRREYTLLLPFAPGARLPAAAVAAAATGVAVARRARRDRARDALRPGHRP"
     gene            1184..2344
                     /gene="sucC"
                     /locus_tag="PKV97_16170"
     CDS             1184..2344
                     /gene="sucC"
                     /locus_tag="PKV97_16170"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNB07470.1"
                     /translation="MKIHEYQAKELLRKYGVVTPRGFHCVSVDAAVKAAEELGGKIWV
                     VKAQIHAGGRGKGGGVKLARSLDEVRQHANDILGMQLVTHQTGPEGQKVRNLLIEEGA
                     DIKHEYYVAALTDRATQTVAMMASSEGGMDIEEVAHNTPEKIIKVFVDPLVGLTDAQA
                     AELAKGIGVPEGSVAKAVDAFKKLYTCYMETDASLAEINPLILEGNGNIKALDAKFNF
                     DSNALYRHPEIVDFRDFDEEDADEIEASKYDLAYISLDGNIGCLVNGAGLAMATMDTI
                     KLFGAEPANFLDVGGGATTEKVTEAFKIMLKNPKVKGILVNIFGGIMRCDTIATGVVA
                     AAREVNLSVPLVVRMKGTNEDLGKKILADSGLPIIAADTMAEAATKIVAAVK"
BASE COUNT          445 a          822 c          815 g          351 t
ORIGIN      
        1 gccggcgtgc ccgtcgccga cgcggagacg cagacccgcg gcgcgacgac ggtcagcgac
       61 cctacggcct gggggtcgtc gccgtcgacg atcgcgacct cgacgccggt cacagcgtcg
      121 ccgcgggtca cgagccggcg ggcgtcgtag atgtcggcgc tgccgttggc gaacgcgccg
      181 ccgtggatcc acaccatgac cgggcgagcg gcggcacccg ccggtgagaa cacgttgagc
      241 gagaggcagt cctcgccgac cgggcgcccg tagtccgggt cacggacggt gtcctggacg
      301 cagcgcgtcc cgggcgcggt cgcgtcgcgc acgtcgggcc acggcgacac cggttgcggc
      361 ggctgccacc gcagccgccg gcaggcgggc gccgggtgcg aagggcagca gcagcgtgta
      421 ctcgcgccgc aggcgcgcct cgcacacgtt ctcgatcgcc agcttcagca ccggctcgtt
      481 gacgcgggcg gcgtcccgca ccaccaggcg cgcttcggca ccgctgccgc tgacggcgat
      541 acggccctgg cgcaaggccg gaaactctcc accggagagg gggacgacgc gaaagcattc
      601 ggcatcggca ggttttccgt ccggcaggcg gatctccgcg cggaagctct cccccatcgc
      661 ggagagtgcg gcgacctcgc cgagcgccac cgccgccgcg ggaggggcgg tgccgagcga
      721 ggcgagggcg attgcgactg cgattgcgag cggcatgctg ttgttgttca tattccgcgc
      781 gtccggcggt ggatcgaatg attgccgagt tttagcagac tcgccgtagg cattccagcg
      841 actcgtgccg caatgcgacc gtgttttgtt gcaggtgtga cgtattcagc gttcccggat
      901 gcttccgcca cacggcagcc ggcgcggtgc gggatgcggg tggagcgggc ccggtgcggc
      961 tgccggcgca tggctgtgca ccgcgcaaaa ccagttataa ttatggggtc ttgcgaccgc
     1021 ggcgtgcgcg cccggtccgc gggtcgggtc ggggggtccc gattccagtg cggcgggcga
     1081 cggtcgaggg agacggcgcc ggtcgtggag cgatcgctcc gaagcatgcc atcaacctgt
     1141 ccgcggcggc ggctgccgtc gcggcttcga tcgaagggca accatgaaaa ttcatgagta
     1201 tcaggcaaaa gaactgttga ggaagtacgg cgtcgtcacg ccgcgcggtt tccactgcgt
     1261 atcggtggac gctgccgtga aggcggccga ggaactcggc ggcaagatct gggtggtcaa
     1321 ggcccagatc cacgccggcg gccgcggcaa gggcggcggc gtcaagctcg cccgctcgct
     1381 cgacgaagtg cgccagcacg ccaacgacat cctcggcatg cagctcgtga cccaccagac
     1441 cggccccgaa ggccaaaagg tgcgcaacct gctgatcgag gaaggcgccg acatcaagca
     1501 cgaatactac gtcgcggcgc tgaccgatcg tgccacccag acggtcgcca tgatggcctc
     1561 ctccgaaggc ggcatggaca tcgaggaagt cgcccacaac acccccgaga agatcatcaa
     1621 ggtcttcgtc gatccgctcg tcggcctcac cgacgcgcag gccgccgagc ttgccaaggg
     1681 catcggcgtg ccggaaggct cggtcgccaa ggcggtcgac gccttcaaga agctctacac
     1741 ctgctacatg gaaaccgatg cctcgctggc ggaaatcaac ccgctgatcc tcgagggcaa
     1801 cggcaacatc aaggcgctcg acgcgaagtt caacttcgac tccaacgccc tctaccgtca
     1861 cccggagatc gtcgatttcc gcgacttcga cgaagaggat gccgacgaga tcgaagcctc
     1921 caagtacgac ctcgcctaca tcagcctcga cggcaacatc ggctgcctgg tgaacggcgc
     1981 cggcctggcg atggccacca tggacacgat caagctgttc ggcgccgagc cggccaactt
     2041 cctcgacgtc ggcggcggtg ccacgaccga gaaggtcacc gaagcgttca agatcatgct
     2101 caagaacccc aaggtcaagg gcatcctggt caacatcttc ggcggcatca tgcgctgcga
     2161 caccatcgcc accggcgtgg tcgccgcggc ccgcgaagtg aacctctccg tccccctcgt
     2221 ggtgcgcatg aagggcacca acgaagacct cggcaagaag atcctcgccg attcgggcct
     2281 gccgatcatc gccgccgaca cgatggcgga agccgcgacc aagatcgtcg ctgcggtcaa
     2341 gtaaggagca cttcgaatgt ccatcctgat caacaaagac accaaggtca tcacccaggg
     2401 cattaccggc aagaccggcc agttccacac cga
//
LOCUS       DAOQNN010000464         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_34384_length_2417_cov_8.464437, whole genome shotgun sequence.
ACCESSION   DAOQNN010000464 DAOQNN010000000
VERSION     DAOQNN010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34384_length_2417_cov_8.464437"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PKV97_16175"
     CDS             <1..284
                     /locus_tag="PKV97_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07471.1"
                     /translation="PAALPLAEQPREVDEHFARTLREAMAQGDQLHPADLAATRRAVH
                     APPEAAQPLAFRAARFSDGSLATERDGVVVEVYSAAETDAIQKLLGVRT"
     gene            281..1129
                     /locus_tag="PKV97_16180"
     CDS             281..1129
                     /locus_tag="PKV97_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNB07472.1"
                     /translation="MRDPFLIDGPTCISFSGGRTSAYMLWRVLDSNGGLPDEAQVCFE
                     NTGKEDEATLRFVRECGSRWGVRIRWLEYRTAVVPADRWAKVDFDSASRDGEPFEAAI
                     RQRQFLPNPITRFCTVELKIRTAHRFLRSLGWTEWDQMVGIRADEQRRVAKIRARPSP
                     EIAAETMTMPLADAGVTLADVHAFWSQQSFGLELRTVNGRTLAGNCDLCFLKPAAQVM
                     SLIAEKPARAAWWARMEALALASTADGARFRNDRPSYSQMAQFAVQQGDLFDHDEEAL
                     ACFCGE"
     gene            1132..1368
                     /locus_tag="PKV97_16185"
     CDS             1132..1368
                     /locus_tag="PKV97_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07473.1"
                     /translation="MTPQDALQTSCAGKGRFPTQQQAAERAKRMRRRNGRERPATVYQ
                     CFFCHGWHIGGKRPDTVDGNKAFKRRRQQAMEQQ"
     gene            1368..2345
                     /locus_tag="PKV97_16190"
     CDS             1368..2345
                     /locus_tag="PKV97_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07474.1"
                     /translation="MARARNIKPGFFNNEDLAECSLAARLCFAGLWTLADREGRLEDR
                     PKRIKGELFRFDSLDVEPLLKELAHRGFILRYEVDGRGLIQVVAFHKHQNPHHREPPS
                     ELPPPPSPGLGPDANTPKPRAPGSSDGAEAPDRPKASPGLDPPRDDLPPPLSRVDSGA
                     LIPDSGSEEDVPPSPPARDEDGPGEDDPPPVTPTAAGIACRAMRQAGLTATNPSDPRL
                     HALLEVGVTADELAAIAAEAVTKGKGNAWSWVLAVAKARRDDAGQIAATTAAAPPDPM
                     AWRKTFEGVQRRAAELGLKPRTGEMAPDFERRVLTAHQRAASTAQGSPA"
BASE COUNT          413 a          869 c          822 g          313 t
ORIGIN      
        1 cccctgctgc gctgcccctt gccgagcagc cgcgcgaggt cgacgagcac ttcgcacgca
       61 cgctgcgcga ggcgatggcg cagggcgacc agctgcaccc cgccgacctg gccgccacgc
      121 gccgcgccgt gcacgcgcca cccgaggccg cgcagccgct cgcattccgc gccgcgcgct
      181 tctccgacgg ctcgctggcc accgagcgcg acggtgtcgt ggtcgaggtc tacagcgcgg
      241 ccgagaccga tgcgatccag aagctgctgg gggtgcggac gtgagggacc ccttcctgat
      301 cgacggcccg acgtgcatca gcttcagcgg cgggcgcacg agcgcttaca tgctgtggcg
      361 cgtgcttgac tccaacggag gactgcccga tgaagcacaa gtctgcttcg agaacacagg
      421 caaggaagac gaggccacgc tgcgcttcgt ccgcgagtgc ggatcccggt ggggagtacg
      481 tattcgatgg cttgaatatc ggaccgcggt ggtgcccgcg gatcgctggg ccaaggttga
      541 cttcgactct gcatcgcgcg acggcgagcc gttcgaagca gcgataaggc agcgtcagtt
      601 cctgccgaac ccgatcacgc ggttctgcac ggtcgagctg aagatcagga ccgcgcacag
      661 gttcctgcgc tcgctcggct ggaccgagtg ggaccagatg gtcggcatcc gcgcggacga
      721 gcagcgccgc gtggccaaga tccgcgccag gcccagcccg gagattgcgg ctgagacgat
      781 gacgatgccg ctggccgatg ctggcgtgac gctggccgac gtgcatgcgt tctggagcca
      841 gcagtcgttc ggccttgagc tgcggaccgt caacggccgc actttggccg gcaactgcga
      901 cttgtgtttc ctcaagcccg cagcgcaggt catgtcgctg atcgccgaga agccggcgcg
      961 tgcagcgtgg tgggctcgca tggaggcgct ggcgctggcg tcgacggcgg acggagcccg
     1021 tttccgcaac gaccgcccga gctacagcca gatggcgcag ttcgcggtcc agcagggcga
     1081 cttgttcgac cacgacgagg aagcgctcgc gtgcttctgt ggggagtgag catgaccccg
     1141 caagacgccc tccagacctc ctgcgccggc aagggccgct tcccgaccca gcagcaggcc
     1201 gccgagcgcg cgaagcgcat gcgccgccgt aacggccgcg agcgcccggc cacggtctac
     1261 cagtgcttct tctgccacgg ctggcacatc ggagggaagc gcccggacac ggtcgacggc
     1321 aacaaggcgt tcaagcggcg ccggcaacag gcgatggagc aacagtagtg gcccgcgcac
     1381 gcaacatcaa gcccgggttc ttcaacaacg aggatctggc cgagtgctct ctcgccgcga
     1441 ggctgtgctt cgccgggctg tggaccctgg ccgatcggga agggcgtctc gaggacagac
     1501 cgaagcggat caagggcgag ctgttcaggt tcgacagcct ggacgtcgag ccgctgctga
     1561 aggagcttgc gcaccgcggc ttcatcctgc gctacgaggt cgacggccgc ggcttgatcc
     1621 aggtggtcgc gttccacaag caccagaacc cccaccacag ggagcctcca agcgagttgc
     1681 caccaccacc aagcccaggg ctcgggcctg atgccaacac accaaagccc agggccccag
     1741 gatcatccga tggagccgag gccccggaca ggcccaaggc aagcccaggg cttgaccccc
     1801 caagagacga cttgccaccc cccctcagcc gtgtggattc cggagccctg attcctgatt
     1861 ccggaagcga ggaagacgta ccgccatcgc cccccgcgcg cgatgaggat ggccccgggg
     1921 aggatgaccc gcctccggtg acgcccaccg ccgccggcat cgcatgccgg gccatgcgcc
     1981 aggccgggct caccgcgacg aaccccagcg acccgaggct gcacgcgctc ctcgaggtcg
     2041 gcgtcacggc cgacgagctc gccgcgatcg ccgccgaggc ggtcaccaag ggcaagggca
     2101 acgcgtggtc ctgggtcctg gcggtcgcca aggcccgccg cgacgacgcc gggcagatcg
     2161 ccgcgaccac cgcggcagcg ccgcccgacc cgatggcctg gcgcaagacc ttcgagggcg
     2221 tgcagcgccg cgccgccgag ttgggcctga agccccgcac cggcgagatg gccccggact
     2281 tcgagcgccg cgtcctcacc gcccaccagc gcgctgcgag cacagcgcag ggatcccccg
     2341 catgacgacc accaccgcca ccgacccgaa gcgcaccgcc cggcagcttg ccgcggcagc
     2401 ccgcgcggag gaccagt
//
LOCUS       DAOQNN010000465         2414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_34456_length_2414_cov_13.304366, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000465 DAOQNN010000000
VERSION     DAOQNN010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34456_length_2414_cov_13.304366"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PKV97_16195"
     CDS             complement(<1..890)
                     /locus_tag="PKV97_16195"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343151.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNB07475.1"
                     /translation="MKSLLADLFKQTLPNEEEFDAITIGLASPDKIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTLSKVRRE
                     RMGHIELASPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVVEPGMTPLNRGQ
                     LLTEDDYLAKVEEYGDEFDAVMGAEGIGSLLRTLDINLEAEKLRGDLETTNSEAKIKK
                     FSKRLKVLEAFQQSGIKPEWMVLEVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELKAPDIIVRN"
     gene            complement(976..>2414)
                     /gene="rpoB"
                     /locus_tag="PKV97_16200"
     CDS             complement(976..>2414)
                     /gene="rpoB"
                     /locus_tag="PKV97_16200"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298411.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNB07476.1"
                     /translation="AEVEAGDVLVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDTSL
                     RVPSGMVGTVIDVQVFTREGIERDKRAQSIIDEQLRSFKTDLADQMRIVERDAFARIR
                     RMIVGQLANGGPKKLAKGAALTDPYLDSLEHYHWFDIRLADETLAVQLEAVREGLENT
                     RKDFEQAFEIKKKKLTQGDELPPGVQKMVKVYLAVKRRLQPGDKMAGRHGNKGVVSKI
                     VPVEDMPYMEDGTPLDIVLNPLGVPSRMNIGQILETHLGWAAKGLGQKIDKMLQAQAS
                     ALELRGFLEQIYNGSGHPEDIANLGDSDVRELAGNLKKGVPFATPVFDGAKEEEIQQM
                     LELAGLPTGGQVTLYDGRTGEAFERKVTVGYKHVLKLHHLVDDKMHARSTGPYSLVTQ
                     QPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVTGRTKVYENIVKGEHK
                     IDAGMPESFNVLVKEIRSLAIDIDLDRE"
BASE COUNT          366 a          778 c          792 g          478 t
ORIGIN      
        1 tcgttgcgca cgatgatgtc gggcgccttg agctcgagca gacgcttgag gcggttgttg
       61 cggttgatga cgcgacggta gaggtcgttc agatccgagg ttgcgaagcg gccaccgtcc
      121 agcggcacca gcgggcgcag gtcgggcggc agcaccggca gcacctcgag caccatccac
      181 tcgggcttga tgcccgactg ctggaaggcc tcgagcacct tcaggcgctt ggagaacttc
      241 ttgatcttgg cttcggagtt ggtggtctcc aggtcgccac gcagcttctc ggcctcgagg
      301 ttgatgtcga gcgtgcgcag cagggaaccg ataccctcgg cacccatgac cgcgtcgaac
      361 tcgtcaccgt actcctcgac cttggcgaga tagtcgtcct cggtcagcag ctggccgcgg
      421 ttgagcggcg tcatgcccgg ctcgacgacg acgaaggctt cgaagtagag cacgcgctcg
      481 atatcgcgca gggtcatgtc gagaaccatg ccgagacggc tcggcaagct cttcaggaac
      541 cagatgtggg cgaccggcga ggccagctcg atgtggccca tgcgctcgcg ccgcaccttc
      601 gacagcgtca cctcgacgcc gcacttctcg cagatcacgc cacggtgctt gaggcgcttg
      661 tacttgccgc acaggcactc gtagtccttg accggcccga agatcttggc gcagaacagg
      721 ccgtcacgct cgggtttgaa cgtgcggtag ttgatcgtct cgggcttctt gacttcgcca
      781 taggaccacg agcggatctt gtccggcgag gcgaggccga tcgtgatcgc gtcgaactct
      841 tcctcgttcg gcagggtttg cttgaacagg tcagcaagca ggcttttcat ggttcactcc
      901 atccgggccg ccgcgtcgtg ggcggcggcc ccgggattca atcgcaggca ggggacggga
      961 aggaaatcgc gacgctcact cgcggtcgag gtcgatgtcg atcgccagcg aacggatttc
     1021 cttcaccagc acgttgaagg attccggcat gccggcatcg atcttgtgct cgcccttgac
     1081 gatgttttcg tacaccttgg tccggccggt cacgtcgtcg gacttgaccg tcagcatctc
     1141 ctgcagcgtg taggcggcgc cgtaggcttc cagcgcccac acttccatct cgccgaagcg
     1201 ctggccgccg aactgcgcct tgccgcccag cggttgctgt gtgacgagcg agtacgggcc
     1261 ggtcgaacgc gcgtgcatct tgtcgtcgac caggtggtgc agcttcagca catgcttgta
     1321 gcccacggtg accttgcgct cgaaggcctc gccggtgcgg ccgtcgtaga gggtgacctg
     1381 gccgccggtg ggcaggccgg ccagctcgag catctgctgg atttcctctt ccttggcacc
     1441 gtcgaacacc ggggtcgcga agggcacgcc cttcttgagg ttgcccgcga gctcgcgaac
     1501 gtccgagtcg ccgaggttgg cgatgtcctc ggggtggccg ctgccgttgt agatctgctc
     1561 gaggaagccg cgcagctcca gcgccgaggc ctgcgcctgc agcatcttgt cgatcttctg
     1621 accgagtccc ttcgctgccc agccgaggtg ggtctcgagg atctggccga tgttcatccg
     1681 cgacggcacg ccgagcgggt tgagcacgat gtcgagcggg gtgccgtctt ccatgtacgg
     1741 catgtcctcg accgggacga tcttggagac cacgcccttg ttgccgtgac ggccggccat
     1801 cttgtcgccc ggctgcaggc ggcgcttcac cgccagatac accttgacca tcttctgcac
     1861 gcccgggggg agctcgtcgc cctgggtgag cttcttcttc ttgatctcga aggcctgctc
     1921 gaagtccttg cgagtgttct ccagaccttc acgcacggcc tcgagctgca ccgcgagggt
     1981 ctcgtcggcg aggcggatgt cgaaccagtg gtagtgctcc aggctgtcga ggtagggatc
     2041 ggtgagcgcc gcacccttgg cgagcttctt cggaccaccg ttggcaagct ggcccacgat
     2101 catgcggcgg atacgcgcga aggcgtcgcg ctcgacgatg cgcatctggt cggcgaggtc
     2161 ggtcttgaag ctgcgcagct gctcgtcgat gatcgactgc gcgcgcttgt cgcgctcgat
     2221 gccctcccgg gtgaacacct gcacgtcgat gacggtgccg accatgcccg agggcacgcg
     2281 cagcgaggtg tccttcacgt cggaggcctt ctcgccgaag atggcgcgca ggagcttctc
     2341 ttccggcgtc agctgggttt cgcccttggg cgtcaccttg ccgaccagca cgtcgccggc
     2401 ttccacctcg gccc
//
LOCUS       DAOQNN010000466         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_34485_length_2413_cov_7.921120, whole genome shotgun sequence.
ACCESSION   DAOQNN010000466 DAOQNN010000000
VERSION     DAOQNN010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34485_length_2413_cov_7.921120"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PKV97_16205"
     CDS             complement(<1..653)
                     /locus_tag="PKV97_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HNB07477.1"
                     /translation="MCRSVTAGARSLFKCHIDQDTASRPAREVTAHEVAALVRRVREA
                     GKERAAGVLRSYLSAAFNAAKRAPFDSALPADLIAFGLEHNPVDAIPAIAVQRGDRTL
                     AAGELRAYIGHLLRIGHETGPELADMALLLALYAGGQRMAQLLRAKVGDFDQDTATLR
                     LWDGKGKRTQPREHLLPLAPHAAGMVAALVERAKARETERAKQEGRAPELAGLWLFST
                     "
     gene            815..1288
                     /locus_tag="PKV97_16210"
     CDS             815..1288
                     /locus_tag="PKV97_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07478.1"
                     /translation="MARRSIEMYEYRQALMRMRQGDSEREIARSKLMGRSKAARFREP
                     ARAQGWLDPERPLPEDAEIAAALGQPKLPATAQSSIAAYRPLVEQWLAQGVSGVVIHT
                     TLKREHGFSGHYSSVRRLIAQIGRPPACEARWRAPVDKPMPSPEMTVIVTDAVKL"
     gene            complement(1369..1668)
                     /locus_tag="PKV97_16215"
     CDS             complement(1369..1668)
                     /locus_tag="PKV97_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07479.1"
                     /translation="MTELAALKPRILANLESGRRLSVPPELRDHLERITEMVAEHYQA
                     HHGEVVLWGKIDHYRHHDGRGTVYRISPDGRLLDESGAARVGLERRAELSVGVVF"
     gene            complement(1814..>2413)
                     /locus_tag="PKV97_16220"
     CDS             complement(1814..>2413)
                     /locus_tag="PKV97_16220"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNB07480.1"
                     /translation="HRKEYTMNVSPEKMLSAFRSTADIRVHELAVASDSLLSGVEHLT
                     ALNLNTGRALLDEAIAFTQAAASAREPRTLVELQVGSITPVLRHGHAWFTTAGTVAGQ
                     AQADALRLFEAGMSQWLQGVILTLELLHRSAPAGSALTVQALRSVVANASSAYDGATR
                     AAAHATATARTHADQAASAALEALDRSTSATVSLIRSAA"
BASE COUNT          361 a          800 c          862 g          390 t
ORIGIN      
        1 gtcgagaaaa gccacaggcc ggccagctcg ggcgcgcggc cttcctgctt ggcgcgctcg
       61 gtctctcggg ccttggcgcg ctccacaagg gcggccacca tcccggctgc gtgcggtgcc
      121 agcggtaata ggtgttcgcg gggttgcgtg cgcttgccct tgccatccca caggcgaagc
      181 gttgcggtgt cctggtcgaa gtctcccacc ttggcgcgca acagttgcgc catgcgttgc
      241 ccacccgcgt acagcgccag cagcaaagcc atgtcggcca gctcggggcc ggtctcgtgc
      301 ccgatgcgca gcaggtggcc gatgtacgcg cgcagttcgc cagcggctag cgtgcggtcg
      361 cctcgttgca cggcaattgc cggaatggcg tcgacggggt tgtgttcgag gccgaatgca
      421 atcaggtcgg caggaagggc ggaatcgaag ggcgcacgct tggcggcatt gaatgcggcg
      481 gacaggtagc tgcgcagaac tccggcggcg cgctccttgc cggcttccct cacccggcgc
      541 accagcgcgg ccacctcgtg cgcggtgact tcgcgagcgg gcctgctcgc ggtgtcctgg
      601 tcgatatggc acttgaacag gctccgcgct ccggctgtca ccgaccggca cattggagcc
      661 agttgatgat cggcacaatg gagccaccgc gtgatcggca catagaggcc agcgccggat
      721 cggcgtaacg gagccacttc cactctgacg gacgtctcga ccttgagcgg tccatcgcta
      781 ccctgctgcc tttttgttcg gcaggaggta ggcggtggcc cgcaggagca tcgagatgta
      841 cgagtaccgc caggccctga tgcgcatgcg ccagggtgac tccgagcgcg agatcgcgcg
      901 ctcgaaactg atggggcgca gcaaggcggc ccgcttccgc gagccggctc gcgcccaggg
      961 ctggctcgat cccgagcgcc ctctgcccga ggacgccgag atcgccgccg cgctcgggca
     1021 gccgaagctg ccggcgacgg cccagtcctc gattgccgcg taccggccgc tggtcgagca
     1081 gtggctcgcg cagggcgtct ccggcgtggt gatccacacc acgctcaagc gcgagcacgg
     1141 gttcagcggg cactactcca gcgtgcgccg gctgatcgcg cagatcggcc gcccgccagc
     1201 gtgtgaggcg cgctggcggg cgcctgtgga taagcctatg ccatcccccg aaatgaccgt
     1261 gatcgtgacc gacgcggtta aactctgaac cccacgctgg agcgcacaga acaccggtca
     1321 cgttcgtcgg tatgagcggt cacgatcgtc ggaatgcgca gtgacggttc agaaaacgac
     1381 tccgacagac agctctgcac gacgttctaa acctaccctt gccgcccctg attcatcaag
     1441 cagccgcccg tccgggctga tccgatacac cgttccgcgg ccgtcgtgat ggcggtagtg
     1501 gtcgattttt ccccagagca ccacctcgcc gtgatgtgcc tggtagtgct ccgcaaccat
     1561 ctccgtgatg cgttcgaggt ggtcgcgcag ttcagggggt acgctgaggc gcctgccgct
     1621 ctcaaggttc gccaggatgc ggggcttcag ggcggcaagc tcagtcatcg gtcaggcgct
     1681 catcatctcg ggcgcacgct gcaccgcagg catacctgca gcgcaggccg ccgccattcc
     1741 cttccgctct tgcacgcgca agtgccgccc caccttccac cgcgcggccc cctgcctcgg
     1801 gggcccgccc ggctcaggcc gcgctgcgga tgagcgagac cgtggcgctg gtgctgcggt
     1861 cgagggcctc gagcgcggca ctggcggcct ggtcggcgtg ggtacgcgcg gtggcagtgg
     1921 cgtgggcggc tgcacgagtg gcaccgtcgt aggcggacga ggcgttggcg accaccgagc
     1981 ggagggcctg cacggtgagc gcggagcccg cgggggcgct gcggtggagc agctcgaggg
     2041 tgaggatcac gccctgcagc cattgcgaca tgccagcctc gaacaggcgc agcgcgtcgg
     2101 cctgggcctg gccggcgacc gtgccggcgg tggtgaacca ggcgtggccg tgacgaagca
     2161 ccggggtgat cgagccgacc tggagctcga ccagggtgcg cggctcgcgc gccgacgcgg
     2221 cagcctgggt gaaggcgatg gcttcgtcga gcagcgcgcg gccggtgttg aggttgagcg
     2281 cggtcaggtg ctcgaccccg gagagcaggc tgtcgctcgc gacggcgagc tcgtggacgc
     2341 ggatgtccgc ggtgctgcgg aaggcggaaa gcattttctc gggcgagacg ttcatggtgt
     2401 actccttgcg gtg
//
LOCUS       DAOQNN010000467         2402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_34773_length_2402_cov_11.902855, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000467 DAOQNN010000000
VERSION     DAOQNN010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34773_length_2402_cov_11.902855"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1265)
                     /locus_tag="PKV97_16225"
     CDS             complement(78..1265)
                     /locus_tag="PKV97_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNB07481.1"
                     /translation="MSNSDLDDLAAELAEFAPPEKKNGRPASEERVIAGFEEIQRFVE
                     QHGRAPQHGEDRDIFERLYAVRLDRLRALPDCRALLEPLDHQGLLAGAPTVAASADET
                     IDIEDLAAELADVAGADDITVLRHVRTSAEKRAAEEIADRKPCEDFETFRPLFERVQA
                     EVKTGMRQSQPIEAGRRAIEVGDFFVLDGLTLYVAEVGEPLKTTAGEVDRRLRLIFSN
                     GTESNLLLRSLQRAFYNDPAARRLVSPESGQLSFGGELDADDIESGTIYVLRSLSDHP
                     YVAQHRDIIHKLGVTGGRVETRIANAENEATYLLAKVEVVATYKLAGINRARMENLFH
                     RLFAPARLNITINDRFGHPVQPEEWFLVPLFVIDEAVARIKDGSITGYIYDPQAAKLV
                     KAG"
     gene            complement(1258..>2402)
                     /locus_tag="PKV97_16230"
     CDS             complement(1258..>2402)
                     /locus_tag="PKV97_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004541851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB07482.1"
                     /translation="PDGRVLRIADLVDDDAAKRDRVSVSLKDPAQKNNRDHVDIIIAL
                     GMAKEGFDWIWCEHALTVGYRASLTEIVQIIGRATRDAPGKTRARFTNLIAEPDAAEE
                     AVTEAVNDTLKAIAASLLMEQVLAPRFEFKPKNPDSGPTPGFNYGEGGYDSDRCNVGV
                     NEQTGAYQIEIKGLAEPKSKEAARICQEDLNEVIAAFVQDKPTIERGLFDEELVPEEL
                     TQVRMGKIIKDKYPELDAEDQEAVRQHAIAALNLTQQAKRLVTEGESDGSPNTALIDG
                     VRRFAMDVRELDIDLIDRINPFGEAYAILAKTMSEDSLKQVAAAISAKRTSITPEDAK
                     VIAKRAVEFKRERGRAPSVTSQDAWEKHLAEGAAAFMRFRAEGRYE"
BASE COUNT          389 a          761 c          773 g          479 t
ORIGIN      
        1 ggaactaaac atcaccccga ccgaactggc ccggcagatc gcggtgccgg ccaatggtgc
       61 gatggagctg tctcgaccta ccccgccttc accagctttg ccgcttgggg atcgtagatg
      121 tagccggtga tgctgccatc cttgatgcgc gcgaccgcct cgtcgatgac gaacagcggc
      181 acgagaaacc actcctcggg ctgcacggga tggccgaagc gatcattgat ggtgatgttc
      241 aaccgcgcgg gcgcaaacag ccggtggaac aggttttcca tccgggcgcg gttgatgccc
      301 gcgagcttgt aggtggcgac gacctcgacc ttggcgagca ggtaggtcgc ttcgttctcg
      361 gcgttggcga tgcgcgtctc cacccggccg ccagtcacgc ccagcttgtg gatgatgtcg
      421 cggtgctgcg cgacgtatgg atgatccgac agcgagcgca ggacgtagat cgtgccgctc
      481 tcgatgtcgt ccgcgtccag ctcgccgccg aaggacagtt gtccactctc cggcgagacc
      541 agccgccgcg cggcggggtc gttgtaaaaa gcgcgctgga gcgagcgcag cagcagattg
      601 ctctcggtgc cgttggagaa gatgaggcgc aggcggcggt ccacttcccc ggcggtggtt
      661 ttcaacggct cgccgacctc ggcgacgtag agagtaaggc cgtcgagaac gaaaaagtcc
      721 ccgacttcaa tcgcacggcg gcccgcttcg atgggctggg actggcgcat accggttttg
      781 acttccgctt gcacgcgctc gaacaagggc cggaaggtct cgaagtcctc gcagggcttg
      841 cgatccgcga tctcctcggc ggcgcgcttt tcggcgctgg tacggacatg gcgcaagacc
      901 gtgatgtcgt ccgcgccggc aacatccgcc agttcggcag ccagatcttc gatgtcgata
      961 gtttcgtccg ccgaagcggc aacggtcggt gccccagcaa gcaaaccctg atggtctagc
     1021 ggctcaagca aggcgcggca atccgggagc gcgcgtagac ggtcaagccg cacggcatat
     1081 aggcgctcga atatgtctcg gtcttcgcca tgctgcggcg cgcgcccgtg ctgctctacg
     1141 aagcgctgga tttcctcgaa gccagcgatg acacgctcct cgctggctgg acgtccattt
     1201 ttcttctcag gtggtgcgaa ctcggccagc tccgccgcca ggtcgtcaag atcggagtta
     1261 ctcatagcgc ccctccgccc tgaaacgcat gaaggcggcc gcgccttcgg caaggtgctt
     1321 ttcccaagcg tcttgcgagg tcaccgacgg agcccgtcca cgctcgcgtt tgaactcaac
     1381 tgcgcgcttg gcgatcactt tggcatcctc aggcgtgatc gaggtgcgct tggccgagat
     1441 ggccgctgcg acctgcttca agctgtcctc gctcatggtc ttggcgagga tggcgtaggc
     1501 ttcgccaaag gggttgatgc ggtcgatcag gtcaatgtcc agctcgcgca cgtccatcgc
     1561 gaagcggcgc acgccgtcga tcagggcggt gttgggcgac ccgtcgctct cgccttcggt
     1621 gacaagccgc ttggcctgct gcgtgaggtt gagggcggcg atggcgtgct ggcgcacggc
     1681 ctcctgatct tcggcgtcaa gctcgggata cttgtccttg atgatcttgc ccatgcgaac
     1741 ctgcgtcagc tcctcgggca ccagctcctc atcgaacagg ccgcgctcga tggtgggctt
     1801 gtcctgcacg aaggccgcaa tgacttcgtt caggtcttcc tgacagattc gcgccgcctc
     1861 cttgctcttg ggctcggcga ggcccttgat ctcgatttgg tacgcccctg tctgctcatt
     1921 gacaccgaca ttgcagcggt ccgagtcgta gccaccctcg ccgtagttaa aacccggcgt
     1981 cgggccgctg tcggggttct tgggcttgaa ctcgaagcgc ggagcaagaa cctgctccat
     2041 cagcaggctt gccgcgatgg ccttcaacgt atcgttgacg gcctcggtga cagcttcctc
     2101 ggccgcatcc ggctcggcga tcaggttggt gaacctcgcg cgggtcttgc cgggcgcgtc
     2161 gcgggtagct cggccgatga tctgcacgat ctcggtcagg ctggcgcggt agcccacggt
     2221 cagcgcatgt tcgcaccaaa tccaatcgaa gccttccttc gccatgccca gcgcgatgat
     2281 gatgtccaca tggtcgcggt tgttcttctg cgccgggtct ttgagtgaga cggacacgcg
     2341 gtcgcgcttg gcggcatcgt catcgacgag atcggcgatt cgcagcacgc ggccatcggg
     2401 gc
//
LOCUS       DAOQNN010000468         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_34792_length_2401_cov_10.069480, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000468 DAOQNN010000000
VERSION     DAOQNN010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34792_length_2401_cov_10.069480"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2270)
                     /locus_tag="PKV97_16235"
     CDS             complement(<1..2270)
                     /locus_tag="PKV97_16235"
                     /inference="COORDINATES: protein motif:HMM:NF018620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HNB07483.1"
                     /translation="MTGLLSLILILGGCAMLARQALDERFGPTDPARYDQPVPPPRGM
                     SYHGDVQPILERRCVVCHACYDAPCQLKLGAWEGIARGTSTQIVYDGTRLSEAQPSTL
                     FVDAQSISEWRARGFSAVLNERQPTPAANLAAGVLARALQLKQAHPLPDSAILPATFD
                     LSLDRAQQCPRIEEYDTFARENPLWGMPFGLPGLSPPELATLTRWLELGAPFEGLPPL
                     PAPLLTEISGWEAFLNGSSLKERLVSRYLFEHLFLAHLYFESDGQSHFFRLVRSRTPP
                     GQPIDLVASRRPFDDPGVDPVYYRFDRERETIVAKTHMPYSLSPRRMARWRELFLTPD
                     YRVDELPSYAIEVASNPFVTFRDLPVSARYRFMREEAEFTIMGFIKGPVCRGQVALNV
                     IEDRFWVFFLADSDLQTLRAPFLDREASLLALPAAQGSDAAIIGPWREYARRQARYLQ
                     DKSAFLIQYASTRRPDLRWIWNGDGENANAALTVFRHFDSGTVVKGLLGGPPKTAWVV
                     SYPLLERIHYLLVAGFDVYGNIGHQLLTRLYMDFMRMEGEYNLLTFLPLAQRKEVRDL
                     WYLGASQEVKEHVYGSLARFDVDTAIPFRSDQPLQEFYAMLQQRLAPVLDRRHDLSSL
                     TDTVLRDDLEALAALRGPALSWLPELVVLRVDQPGQPAHYFTLLRNTGHRNVSSLLRE
                     GRELLPEDNTLTVVRGILGSYPRAIYRVPRPELRALAGEIGQLASEKDYRALADRYAV
                     RRSDHRFWPVSDELQAA"
BASE COUNT          442 a          756 c          800 g          403 t
ORIGIN      
        1 gcggcctgca gttcgtcgct caccggccag aagcgatgat cgctgcgacg gactgcgtag
       61 cggtccgcca gcgcgcggta atccttttcg gacgcgagtt ggccgatctc gcccgccagg
      121 gcgcggagtt ccgggcgcgg cacgcgatag atggctctgg gataggaacc gaggatgccc
      181 ctgacgacgg tcagcgtgtt gtcttcgggc aacagctctc ggccctcgcg caacagactc
      241 gagacatttc tatgcccggt gttgcgcaac agggtgaagt agtgcgcggg ctgacccggc
      301 tgatcgacgc gcagcacgac cagttcaggc agccacgaaa gtgccggtcc acgcagtgcc
      361 gcgagcgcct ccagatcgtc gcgaagcacg gtatcggtca acgacgagag atcgtggcga
      421 cggtcgagaa ccggtgccag tcgttgctgg agcatcgcgt agaactcctg cagcggttgg
      481 tcactacgga aggggattgc ggtgtcgaca tcgaaacgcg cgaggctgcc gtagacgtgc
      541 tccttgacct cctgactggc gccgagatac cagaggtcgc gcacttcctt ccgctgcgcg
      601 agcggcagga aggtcaggag gttgtactca ccctccatgc gcatgaagtc catgtacagc
      661 cgcgtcaata actggtggcc gatgttcccg tagacatcga aaccagcgac cagcaggtag
      721 tgaatccgct cgaggagcgg atagctgaca acccacgccg tcttcggcgg tccgccaagc
      781 aggcccttga ccacggttcc actatcgaag tggcgaaaaa cggtcagcgc cgcgttcgcg
      841 ttctcgccgt cgccgttcca gatccagcgc aggtcgggcc gccgggtgct cgcgtactgg
      901 atcaggaagg ccgacttgtc ctgcagatag cgcgcttggc gcctggcgta ttcgcgccac
      961 ggcccaatga ttgcggcatc gctcccctgg gcggcgggca gcgcgagcag gctggcttcc
     1021 cggtcgagga acggcgcgcg cagggtctgc agatcgctgt cggcgaggaa gaacacccag
     1081 aagcgatcct cgatcacgtt cagcgcgacc tgcccgcggc agaccggtcc cttgatgaag
     1141 cccatgatcg tgaactcggc ttcctctcgc atgaaacggt agcgcgcact gactggcagg
     1201 tcgcggaagg tcacgaaggg attgctcgcc acctcgatcg cgtacgaagg aagttcgtcg
     1261 acgcggtagt ccggagtcag gaacagctcg cgccagcgcg ccattcgccg cgggctgagc
     1321 gaatagggca tgtgcgtctt ggcgacgatc gtttctcgct cgcgatcgaa acggtaatag
     1381 acggggtcga ctcccgggtc gtcgaacggc cgtcggctgg ccacgaggtc gatcggctgc
     1441 cccggcgggg ttcgcgagcg aacgaggcga aagaaatggc tttgcccgtc gctctcgaaa
     1501 tagagatggg cgagaaacag gtgctcgaag aggtagcgac tcaccagtcg ttccttgagc
     1561 gagcttccgt tcaggaacgc ctcccatccg ctgatctccg tgagcagcgg cgcgggaagc
     1621 ggtggcaatc cctcgaacgg tgctcccagc tcgagccagc gcgtcagtgt cgccagttcc
     1681 ggcgggctca gtcccggcag gccgaaaggc atcccccaca acgggttctc cctcgcaaac
     1741 gtgtcgtact cttcgatccg cgggcactgc tgcgcgcggt cgagcgaaag gtcgaaggtc
     1801 gccggaagaa tcgccgagtc gggcagcgga tgcgcctgct tgagctgaag cgcgcgcgcc
     1861 agcacgccgg ccgcgaggtt cgccgctggc gtcggctgcc gctcgttgag taccgctgag
     1921 aagccacgcg cacgccactc ggaaatactc tgcgcatcga cgaagagcgt cgatggttgc
     1981 gcttcgctga gccgggttcc gtcgtagacg atctgcgtgc tcgtgccgcg ggcgatccct
     2041 tcccaggcgc cgagcttgag ctggcagggg gcgtcgtaac acgcatgaca gacaacgcag
     2101 cgccgttcga ggatcggctg cacgtcgccg tggtacgaca tgccgcgcgg cggtggcacc
     2161 ggctggtcat accgcgcggg gtccgtcgga ccgaatcgtt cgtccagtgc ctggcgagcg
     2221 agcatcgcgc agccgccgag gatcaagatc aacgacagca gcccggtcat ccggctgcaa
     2281 cgcaagaaaa tcgctcgagt tttcatcgtc ttcgaaggag aggaaattca gccgcgatgg
     2341 gcatcactcc gcgaattttc cgctcgtgca tcccgtcgtc aaccggaggg ccggccgcac
     2401 a
//
LOCUS       DAOQNN010000469         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_34833_length_2399_cov_8.007253, whole genome shotgun sequence.
ACCESSION   DAOQNN010000469 DAOQNN010000000
VERSION     DAOQNN010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34833_length_2399_cov_8.007253"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..500)
                     /gene="cueR"
                     /locus_tag="PKV97_16240"
     CDS             complement(102..500)
                     /gene="cueR"
                     /locus_tag="PKV97_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HNB07484.1"
                     /translation="MNIGKAAAASGVSAKMIRYYESIGLVVAARRTDVGYRSYTGEDV
                     HVLRFIRRARDLGFSLADIGDLLALWRDQGRASADVKRIALGHVAALERKIAELQGMV
                     DTLRTLAAHCHGDARPDCPILTDLDAGGGR"
     gene            complement(757..>2399)
                     /locus_tag="PKV97_16245"
     CDS             complement(757..>2399)
                     /locus_tag="PKV97_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HNB07485.1"
                     /translation="RTSDAIRRLMRIQPRTARVRRDAGVVELPIAEVAVGDVVEVRPG
                     EKVAVDGEVVEGASFVDESMITGEPVPVEKGAGATVVGGTLNTRGAFAFRATRVGADT
                     VLAQIIRMVEAAQGAKLPIQALVDKVTMVFVPVVMGVAALTFLAWLLFGAAWLPADVP
                     VLTFALVNAVAVLIVACPCAMGLATPTSIMVATGRAAQMGVLFRKGDALQLLRDAAVV
                     ALDKTGTLTVGKPQLTDLVVAEGFARADVLACVAAVERASEHPIAEAIVVAARAEGLE
                     IAAAEGFGAEPGYGVRARVGGQRVEVGADRLMRRLGLEPGVFADTASRLADEGKSPLY
                     AAIDGRLAAILAVADPLKPSSVAAVRALHDMGLKVAMITGDNARTARAIARQAGIDEV
                     VAEVLPDGKVAALRRLAGRAGDGEAAGRRVAFVGDGINDAPALAEADVGIAIGTGTDV
                     AIESADVVLMSGDLTGVPRAIALSRATMRNIGQNLFWAFAYNSALIPLAAGVFYPAFG
                     ALLSPVFAAAAMSMSSVFVLGNALRLRRFGVDAVQTAG"
BASE COUNT          363 a          944 c          792 g          300 t
ORIGIN      
        1 tctgtttgca ttcagggcgc ttctatcgag ggatgagggg tgcgatggta acccgccgcg
       61 ccactgccct gcgacgatcg ccgccccatc cccgccgccg ctcagcgccc gccacccgca
      121 tccaggtcgg tgaggatggg gcaatccggg cgtgcgtcgc catggcaatg cgccgcaagg
      181 gtgcgcagcg tatccaccat cccctgcagc tcggcgatct tgcgttcgag cgcggcgacg
      241 tgcccgagcg cgatgcgctt gacgtccgcg ctcgcccgcc cctggtcgcg ccacagcgcg
      301 agcaggtcgc cgatgtccgc cagcgagaag cccaggtcgc gcgcgcggcg gatgaagcgc
      361 agcacatgca catcctcgcc ggtgtaggaa cggtagccga cgtcggtccg ccgcgccgcc
      421 accaccaagc cgatcgactc gtagtagcgg atcatcttcg ccgacacgcc ggaggcggcg
      481 gccgccttgc cgatattcat gctgttctcc atgtgccgct cgcgcctccc agtcgccggc
      541 atccaagcgg agtgcccggc catgcgatgc accgacgccc tgcgcatgcc gcgcaggagc
      601 gacgcaagtc gcgattgcag cggcggaaac accgcggcgc ggagaggagc gccggatcgc
      661 gactcgcgtc gctcctaccc aaaccaccgt gcatccaaag cactgcgact tcccgcacgc
      721 aaggtgatgc ggctcgccgc ggctcgtggc ctgcgctcac ccggccgtct gcactgcgtc
      781 gacgccgaag cggcgcaggc gcagcgcgtt gccgagcacg aacacgctcg acatcgacat
      841 cgccgccgcg gcgaacaccg gcgacagcag cgcgccgaag gccgggtaga acacccccgc
      901 ggcgagcggg atcagcgcgc tgttgtaggc gaaggcccag aacaggttct ggccgatgtt
      961 gcgcatggtc gcgcgcgaca gcgcgatcgc gcgcggcacg cccgtcaggt cgcccgacat
     1021 cagcaccacg tcggcgctct cgatcgccac gtcggtgccg gtgccgatcg cgatgcccac
     1081 gtcggcctcg gccagcgccg gcgcgtcgtt gatgccgtcg ccgacgaagg cgacgcgacg
     1141 gcccgccgcc tccccgtcac cagcgcggcc cgccagcctg cgcagcgcag ccaccttgcc
     1201 gtcgggcagc acctccgcga caacctcgtc gatccccgcc tggcgggcga tcgcgcgcgc
     1261 ggtgcgcgcg ttgtcgccgg tgatcatcgc gaccttcagc cccatgtcgt ggagcgccct
     1321 caccgccgcg accgagctcg gcttgagcgg gtcggccact gcaaggatgg cggcgaggcg
     1381 gccgtcgatc gcggcataga gcggcgactt gccctcgtcg gcgaggcgag acgcggtgtc
     1441 ggcgaacacg cccggctcca gccccagccg gcgcatcagg cggtcggcgc cgacctcgac
     1501 gcgctgcccg cccacccgtg cgcgcacacc gtagccgggc tcggccccga agccctccgc
     1561 ggcggcgatc tccagccctt ccgcacgcgc cgcgacgacg atggcctcgg cgatcggatg
     1621 ctccgaggca cgctcgaccg ccgccacgca tgcgagcacg tcggcacgcg caaagccctc
     1681 ggcaaccacc aggtcggtga gctgcggctt gccgacggtg agcgtgccgg tcttgtccag
     1741 cgccaccacg gccgcgtctc gcagcagctg cagcgcgtcg cccttgcgga acagcacgcc
     1801 catctgcgcc gcgcgcccgg tggcgaccat gatcgaggtc ggcgtcgcca gccccatcgc
     1861 gcacgggcag gcgacgatca gcaccgccac cgcattcacc agcgcgaagg tgagcaccgg
     1921 cacatccgcc ggaagccagg cggccccgaa caacagccag gcgaggaagg tgagcgccgc
     1981 cacccccatc accaccggca cgaacaccat cgtcaccttg tcgaccaggg cctggatcgg
     2041 caacttggcg ccctgcgccg cctcgaccat gcggatgatc tgcgcgagca cggtgtccgc
     2101 ccccacccgg gtggcgcgga aggcgaaggc accgcgggtg ttgagcgtgc cgccgaccac
     2161 ggtcgcaccg gcgcccttct ccaccggcac cggctcgccg gtgatcatcg actcgtccac
     2221 gaaggacgca ccctcgacca cctcgccatc gaccgccacc ttctcgcccg ggcgcacctc
     2281 caccacgtcg cccactgcga cctcggcgat cggcagctcc accaccccgg cgtcgcgccg
     2341 cacgcgcgcg gtgcgcggct ggatgcgcat caggcggcgg atcgcgtcgc tggtgcggc
//
LOCUS       DAOQNN010000470         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_34877_length_2397_cov_9.485909, whole genome shotgun sequence.
ACCESSION   DAOQNN010000470 DAOQNN010000000
VERSION     DAOQNN010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34877_length_2397_cov_9.485909"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PKV97_16250"
     CDS             <1..599
                     /locus_tag="PKV97_16250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HNB07486.1"
                     /translation="WSDAKLERAIRRVIVRAEQRAGVGWAKWERIKAARDQRRDAADY
                     DGEALIDKWTKQAVARISGIRDEVAERMRKDIAAALKAGTDPAELAERWRREGLPLDR
                     GTAEGRLRVIAQHQLASLHAQVQSARARALGVRDFVWHTQEDDRVREAHRALHGRVFA
                     YDDPPSEGLPGEPINCRCWAESVVPDEFTIPIGSAFDT"
     gene            686..1390
                     /locus_tag="PKV97_16255"
     CDS             686..1390
                     /locus_tag="PKV97_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07487.1"
                     /translation="MQHSVEIVAWVDVAPTPERIRWAKAQGLAGLNVMVSGDSTDRTA
                     GPFAVHRWVEPTLAAIADAGLDVELTAWAQPHAAYLDAACEWLVGAALRHGARCVWWD
                     AEEPWTQARGGLSPQAAADRIDLAGAREGVTGIGYAPRSLDPLLARADVVAPQAYATT
                     RPGSLSHRGIAGVLEGWRRRAPSAQLEPALAAYSQPSDAGLAQAWVAAGRPSRIQVWA
                     LRHLRAAQLDALAGVG"
     gene            complement(1391..1672)
                     /locus_tag="PKV97_16260"
     CDS             complement(1391..1672)
                     /locus_tag="PKV97_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07488.1"
                     /translation="MTDSACGCLLGQSCSRCRSADGMRRFEQLYLSFSPPWTGRADLA
                     RAATVREFLEMPGRDLPWLACCLLGRRELDAIMLQLILEDLARRGVEVE"
     gene            complement(1669..>2397)
                     /locus_tag="PKV97_16265"
     CDS             complement(1669..>2397)
                     /locus_tag="PKV97_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07489.1"
                     /translation="PVPDLPPPSDLPTDCDATVGGRVLVVGLDTATGDGPTVVYMHGT
                     YETPEGVLATDSAAQAVAAAVEGEGGVLLLPRGRGWWLPGGPWPWGAVTGDAAWMAAD
                     AALVEAALACVPGDPDRVSVAGFSAGAIAAAYLGELRPWASVVLWSGGVQPADRPALP
                     DAPGVMALHGGAGDLAQLREGAIDYAEQAPSLGVLCDHGGGHATAQGTDGAAFLRDAR
                     ASGHPWAAAWPGGWTIDHYCGVVP"
BASE COUNT          353 a          893 c          867 g          284 t
ORIGIN      
        1 tgtggtccga cgccaagctc gagcgcgcga tccgtcgcgt gatcgtgcgt gccgaacagc
       61 gggccggcgt cggctgggcc aagtgggagc ggatcaaggc ggcccgcgat cagcgtcgcg
      121 acgcggccga ctacgacggc gaagcactga tcgacaagtg gaccaagcaa gcggtggcgc
      181 ggatctccgg gatccgcgac gaggtcgccg aacgcatgcg caaggacatc gcagccgcac
      241 tgaaggccgg caccgatccc gctgagctag cggagcggtg gcgccgcgag gggctgccgc
      301 tcgaccgcgg caccgccgag gggcggctgc gggtgatcgc ccagcatcag ctcgcgagcc
      361 tgcacgccca agtccaaagc gcccgcgccc gcgcgctcgg cgtgcgtgat ttcgtctggc
      421 acacacaaga ggacgatcgg gtgcgcgagg cccatcgcgc gctgcatggt cgcgtgttcg
      481 cgtacgacga tccgccgagc gagggtcttc cgggcgagcc gatcaactgc cgatgctggg
      541 ccgagtcggt ggtcccggac gagttcacga tcccgatcgg atcggccttc gatacgtagg
      601 gggctcaagt ccgaggtgca cccggccgat gcgtcccgcg tgcgacgatg gccggaacct
      661 gggcgggtgc tgctagactg tgtacgtgca gcacagcgtc gaaatcgtgg cgtgggtgga
      721 cgtggccccg accccggagc ggatccggtg ggcgaaggcc caggggttgg ccgggctgaa
      781 cgtgatggtc agcggggact cgacggatcg caccgcaggc ccgttcgcgg tgcaccgctg
      841 ggtcgagccc acactggcgg cgatcgctga tgccgggctc gacgtcgagc tgaccgcctg
      901 ggcacagccg cacgcggcct atctcgacgc ggcgtgcgaa tggctggtcg gcgccgccct
      961 gcggcacggc gcgcgctgtg tatggtggga tgcagaagaa ccgtggaccc aggcccgcgg
     1021 cgggctgagc ccccaggccg ccgccgatcg gatcgacctc gcaggcgcgc gcgagggcgt
     1081 gaccgggatc gggtacgcgc cccgctcgct cgacccgctg ctcgctcggg ccgacgtcgt
     1141 cgcaccccag gcctacgcca cgaccaggcc cggcagtctg tcgcaccggg gcatcgctgg
     1201 cgtcctcgag ggctggcgcc ggcgcgcgcc ctcggcccag ctcgagcccg ccctcgcggc
     1261 ctacagccag cccagcgacg ccgggctggc ccaggcctgg gtcgcggccg gtcgcccgag
     1321 ccggatccag gtctgggccc tgcgccacct cagggccgcg cagctcgacg ccctcgcggg
     1381 cgtcggatga tcattcgacc tcgacgcccc gcctggccaa gtcctcgagg atcagctgca
     1441 gcatgatcgc gtccaactcc cgccggccga gcaggcagca cgccagccac ggcagatcac
     1501 ggcccggcat ttcgaggaat tcacgcaccg tcgccgcccg tgccagatcg gcccgaccgg
     1561 tccacggcgg cgagaacgac agatagagct gctcgaatct ccgcatgccg tcggccgatc
     1621 gacaccgcga gcacgactgg ccgagcaggc aaccgcacgc gctgtcggtc acggcacgac
     1681 cccgcagtag tgatcgatcg tccacccgcc gggccacgcg gccgcccatg gatgcccact
     1741 cgcccgcgcg tcgcggagga acgcagcgcc atcggtcccc tgtgcagtcg cgtgcccgcc
     1801 accgtggtcg cacaggacgc cgaggctcgg cgcctgctcg gcgtagtcga tcgcaccctc
     1861 gcgcagctgg gcgagatcgc cggcgccacc atgcagggcc atcacgccgg gcgcgtctgg
     1921 cagcgccgga cggtcggccg gctgcacgcc gccggaccat agcacgaccg acgcccacgg
     1981 ccgcaactcg ccgagatacg cggccgcgat cgcccccgcg ctgaagcccg cgaccgacac
     2041 gcgatctgga tcgccaggca cgcacgcgag agcggcctcg acgagggccg cgtcggcggc
     2101 catccacgcg gcgtcgccgg tgacggcgcc ccaaggccac ggcccgcccg ggagccacca
     2161 gccgcgcccg cgcggcagca gcagcacgcc gccctcgccc tcgaccgccg cggccacggc
     2221 ctgggccgcg ctgtcggtcg caagcacgcc ctcgggggtc tcgtacgtcc cgtgcatgta
     2281 gacgacggtg gggccgtcgc cggtcgcggt gtcgagcccc acgacgagca cgcggccgcc
     2341 gacggtcgcg tcacagtccg tgggcaggtc cgacggcggc ggtagatcgg ggaccgg
//
LOCUS       DAOQNN010000471         2392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_34995_length_2392_cov_9.100556, whole genome shotgun sequence.
ACCESSION   DAOQNN010000471 DAOQNN010000000
VERSION     DAOQNN010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34995_length_2392_cov_9.100556"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /gene="ybgF"
                     /locus_tag="PKV97_16270"
     CDS             <1..418
                     /gene="ybgF"
                     /locus_tag="PKV97_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNB07490.1"
                     /translation="APVPASAPAPAPASANTESADYQGALALLKNGKHKDAQTAFERF
                     ITRYPAGNFTAGAHFWAGNAALQARDVASANRHFKTVMDKYPKENVAPDAMLGLANSQ
                     QAMNDAAGAKRTLQSLVERYPQSNAAQVARQRLSAR"
     gene            422..1093
                     /gene="queE"
                     /locus_tag="PKV97_16275"
     CDS             422..1093
                     /gene="queE"
                     /locus_tag="PKV97_16275"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNB07491.1"
                     /translation="MSAVETAPATAVRLRITEIFASLQGESTRVGLPTVFVRLTGCPL
                     RCTWCDTAYAFSGGETRTLDDILAEVASHGLRHVCVTGGEPLAQKGCLPLLAALCDAG
                     HDVSLETSGALDIAGVDPRVSRIVDLKAPGSGELARNRWENLALLCAHDELKFVLADA
                     ADYAWAKQQIAEHRLAERCGVLLSPVAGALDPAELAGWIVRDRLPVRFQLQLHKILWN
                     DARGR"
     gene            1164..1865
                     /gene="queC"
                     /locus_tag="PKV97_16280"
     CDS             1164..1865
                     /gene="queC"
                     /locus_tag="PKV97_16280"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNB07492.1"
                     /translation="MTSLHTPPRAVVLLSGGLDSATCLAIARDMGFETYALSVAYGQR
                     HAAELAASKRVAQALGAREHRTASVELGQFGGSALTDPGIAVPLDQDNTGIPVTYVPA
                     RNTVMLSIALAWAEVLGANDIFVGVNAVDYSGYPDCRPVFIEAFEHMARLATKVGVEG
                     AQLHIHAPLIELSKADIIRRGTALGVDYGLTVTCYQADDDGRACGRCEACRLRAAGFK
                     AAGVPDPTPYQPRSA"
     gene            1880..>2392
                     /locus_tag="PKV97_16285"
     CDS             1880..>2392
                     /locus_tag="PKV97_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNB07493.1"
                     /translation="MDHALVSASTIVWLGFAIGAAFGASSQRTGFCTMGAVSDAVNIG
                     DWTRMRMWALAVALATLGTGALHALGLFDPAKTLYTGARLPWLSHIVGGLAFGVGMTL
                     ASGCAGKTLIRIGGGNLKSVVVFVFLAIGASMTLRGLTGVWRVEFLDPVALQLPHGQT
                     LPAFLAAAGME"
BASE COUNT          339 a          967 c          749 g          337 t
ORIGIN      
        1 ggcacctgtc cccgcgtccg cacccgcgcc ggcacccgca tccgcgaaca ccgaatccgc
       61 cgactaccag ggcgccctcg cgctgctcaa gaacggcaag cacaaggacg cgcagaccgc
      121 gttcgagcgc ttcatcaccc gctacccggc aggcaacttc accgccggcg cccacttctg
      181 ggccggcaac gccgcactgc aggcgcgcga cgtcgccagt gcgaatcgcc atttcaagac
      241 ggtgatggac aagtacccca aggagaacgt cgcgcccgac gccatgctcg gcctggccaa
      301 cagtcagcag gccatgaacg acgccgccgg cgccaagcgc accctgcaat cgcttgtcga
      361 gcgctatccg cagagcaacg ccgcccaggt cgcccgccag cgcctgagcg cgcgctgatc
      421 catgtccgct gtcgaaacgg cgccggcgac ggccgtgcgc ctccgtatca ccgagatctt
      481 cgcctcgctg cagggcgaat ccacccgcgt cggcctgccc accgtgttcg tgcgcctgac
      541 cggctgcccg ctgcgctgca cctggtgcga caccgcctac gccttcagcg gcggcgagac
      601 acgcacgctc gacgacatcc tcgccgaggt ggccagccac ggtctgcgcc acgtgtgcgt
      661 gaccggcggc gagccgctcg cacagaaggg ctgcctaccg ctgctcgccg cgctgtgcga
      721 tgccggccac gacgtgtcgc tggaaacgag cggcgcactc gacatcgcgg gggtcgatcc
      781 ccgcgtgtcg cgcatcgtgg acctcaaggc cccgggctcg ggcgagctcg cccgcaaccg
      841 ctgggaaaac ctcgcgctgc tgtgcgccca cgacgagctc aagttcgtgc tcgccgacgc
      901 cgccgactac gcatgggcca agcagcagat cgccgagcac cgcctcgccg aacgctgcgg
      961 cgtgctgctg tcgccggtcg ccggcgcgct ggaccctgcc gagctcgccg gatggatcgt
     1021 gcgcgaccgc ctgccggtgc gcttccagct ccagctccac aagatcctgt ggaacgacgc
     1081 ccgcggccgc tgagcgccgc tgttccggcc cggcgacgcc gcgagcagat ccaccccgcc
     1141 ccgccatcca gaatgactcc ggaatgacca gcctccacac cccaccccgc gccgtcgtgc
     1201 tgctctccgg cggcctcgac tccgccacct gcctcgcgat cgcgcgcgac atgggcttcg
     1261 agacctacgc gctgtcggtc gcctacggcc agcgccacgc cgccgagctc gccgcctcga
     1321 agcgcgtggc gcaagcgctc ggcgcgcgcg agcaccgcac cgccagcgtc gaactcggcc
     1381 agttcggggg ctcggcgctc accgaccccg gcatcgcggt gccgctcgat caggacaaca
     1441 ccggcatccc ggtgacctac gtgccggcgc gcaacacggt catgctgtcg atcgcactcg
     1501 cctgggccga ggtgctcggc gccaacgaca tcttcgtcgg cgtgaatgcg gtcgattact
     1561 cgggctaccc cgattgccgg ccggtcttca tcgaggcctt cgagcacatg gcgcggctgg
     1621 ccaccaaggt cggcgtcgag ggcgcgcagc tgcacatcca cgccccgctg atcgagctct
     1681 ccaaggccga catcatccgc cgcggcaccg ccctcggcgt ggactacggc ctcaccgtca
     1741 cctgctacca agccgacgac gacggccgcg cctgcggccg ctgcgaagcc tgccgcctgc
     1801 gcgctgccgg tttcaaagcc gccggcgtgc ccgacccgac gccctaccag ccgcgttcgg
     1861 cctgaccccc tgccgcgcca tggaccacgc cctcgtctcc gcctccacca tcgtctggct
     1921 cggcttcgcg atcggcgccg cgttcggcgc ttccagccag cgcaccggct tctgcaccat
     1981 gggcgcggtc tccgacgcgg tgaacatcgg cgactggacg cggatgcgga tgtgggcgct
     2041 cgcggtcgcg ctcgccaccc tcggcaccgg cgcgctgcac gccctcgggc tcttcgaccc
     2101 tgccaagacc ctctacaccg gcgcccgcct gccctggctg tcacacatcg tgggcggact
     2161 cgccttcggc gtcggcatga cgctcgcctc cggctgcgcc ggcaagaccc tgatccgcat
     2221 cggcggtggc aacctcaagt cggtcgtggt gttcgtcttc ctcgccatcg gcgcctcgat
     2281 gacgctgcgc gggctcaccg gcgtgtggcg ggtcgagttc ctcgacccgg tggcgctgca
     2341 gctgccccac ggccagaccc tgcccgcctt cctcgcggcg gccggcatgg ag
//
LOCUS       DAOQNN010000472         2389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_35074_length_2389_cov_7.453728, whole genome shotgun sequence.
ACCESSION   DAOQNN010000472 DAOQNN010000000
VERSION     DAOQNN010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35074_length_2389_cov_7.453728"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..331
                     /locus_tag="PKV97_16290"
                     /pseudo
     CDS             20..331
                     /locus_tag="PKV97_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(502..1602)
                     /locus_tag="PKV97_16295"
     CDS             complement(502..1602)
                     /locus_tag="PKV97_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB07494.1"
                     /translation="MFFSGPRDRLRASEAENLLLEQANAALQERIRDLEAEQSELRAQ
                     TDALRRERTVLGGVFASLGSFGDSLGGVRESFLGLAGTLNQEKASALEAAAQSDTSRA
                     AFERIAGNLHATFERMGEASGNVGILSRRAEEIGGIVRLIKEIADQTNLLALNAAIEA
                     ARAGDAGRGFAVVADEVRKLAERTAKATTEIATLVENIQDETRAAREVMEFGAADAGR
                     HAADSTQAVSSMKHLLELSRRMERAVTSSALLANVELANIDELALKLEVYKVFLGVST
                     LRPADLPDERHCRLGQWYYDGEGREHFAGLPGYGELEDPHRAVHVHARRAVELHAQGR
                     LEEALAELQAMERANLTVMRGLGRMLDKAVGR"
     gene            1774..2382
                     /locus_tag="PKV97_16300"
     CDS             1774..2382
                     /locus_tag="PKV97_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNB07495.1"
                     /translation="MEFAKIFITLAALINPFGAIPAFIGLTQGQSSLERRRTARTAAL
                     ATVLVITVTALAGQYILAGFGITVPSLQVGGGVLLFIVAISMFNAQPAPSRTTPEETD
                     EAAERANIAVVPLTIPLLTGPGTVSTVIIYADQGTRTVLMLLGAGLVMGVVVWFAFNL
                     AGPISRLAGRTGLNIMTRLMGLVLAALAVEFIAAGIRAYVNA"
BASE COUNT          315 a          845 c          837 g          392 t
ORIGIN      
        1 gcttccggtg tccgccagcg tgggcatcgc cctccatccg cgcgacggcc gcgacccgca
       61 gaccctgctc aagaatgccg acacggcgat gtaccgtgcc aaggagtcgg gccggggcgg
      121 gttctgcttc tacgccgggg agatgaacgc gcaggcgcat cggcgcctcg acctcgagaa
      181 cggcctgcgt cgcgccctcg ccgaggggcg gctggaggtg cattgccggt cccaggtgtc
      241 gcccggggac ggacgcctgt gcggggtgga ggcgctggtg cgctggcgcc atccgcgcga
      301 ggggacgatc gggcccgacg agttcatccc gatcgcggag gagaccggcc tgatcgttcc
      361 gctcggccaa catgtgctgc gcacggcctg cctgcgaggg cgaccggccg ctcttgcccg
      421 cactgcgcgg atccggtcgc cgcagcgccc ggcgcctgag cggcgcccgt cgcgcacgcc
      481 ggatgccggc gcggcgcgta ctcagcgacc gacggccttg tccagcatcc tgccgaggcc
      541 gcgcatcacc gtcaggtttg cccgctccat cgcctgcagt tcggcgagcg cctcctccag
      601 tcggccctgt gcgtgcagtt ccacggcgcg ccgcgcatgc acgtgcaccg cgcgatgcgg
      661 gtcttcgagt tccccgtagc ccggcagccc ggcgaagtgc tcgcgtccct cgccgtcgta
      721 gtaccactgg ccgaggcggc agtgccgctc gtcgggcagg tctgcggggc gcagcgtcga
      781 tacgccgagg aagaccttgt acacctcgag cttgagcgcg agttcgtcga tgttggcgag
      841 ctcgacgttg gcgagcaggg cggaagaggt caccgcccgt tccatgcggc gcgacagctc
      901 gagcaggtgc ttcatgctgg agacggcctg cgtgctgtcc gcggcgtggc gtccggcgtc
      961 ggcggcgccg aactccatga cctcccgcgc ggcgcgtgtc tcgtcctgga tgttctcgac
     1021 cagggtggcg atctcggtgg tggccttcgc ggtgcgctcg gcaagcttgc gcacctcgtc
     1081 cgccaccacc gcgaaacccc gtcccgcgtc gccggcgcgc gcagcctcga tcgccgcgtt
     1141 gagggcgagc aggttggtct ggtcggcgat ctccttgatc aggcggacga tgccgccgat
     1201 ctcctcggcc cgacggctca ggatgccgac gttccccgac gcctcgccca tgcgctcgaa
     1261 ggtggcgtgg agattgccgg cgatccgctc gaacgcggcg cggctggtgt cggactgcgc
     1321 cgccgcttcg agcgccgatg ccttttcctg gttgagggtg ccggcgaggc ccaggaagga
     1381 ttcgcgcacg ccgccgagcg agtcgccaaa gctgcccagg ctggcgaaca cgccgcccag
     1441 cacggtgcgc tcgcggcgca aggcgtcggt ctgcgcgcgc agctcggact gctccgcctc
     1501 gaggtcgcgg atacgttcct gcagggcggc gttggcctgc tcgagcagca ggttctcggc
     1561 ttcgctggcg cgcagcctgt ctctcggtcc ggagaagaac atcgggggct ctggtaggat
     1621 atttaaagtg gagtatcaga tgaatctttt gaccggtcaa gccgcgcatt gcgtccgcgc
     1681 ccacgtcgat ttgcggtggg tccggccggc atcgtagtct gcgcgtcgcg ggacgacggc
     1741 ggggatgccc gcccgcccgc aggagcaaat cgaatggaat tcgcgaagat cttcatcacg
     1801 ctggcggccc tgatcaaccc cttcggcgcc atcccggcct tcatcggcct gacccagggg
     1861 caatcgtccc tcgaacgccg ccgcaccgcg cgtaccgccg cactggcgac cgtcctcgtc
     1921 atcaccgtga ccgcgctcgc cggccagtac atcctggcgg gtttcggcat caccgtgccc
     1981 tcgctgcagg tcggtggcgg cgtgctgctg ttcatcgtcg cgatctcgat gttcaacgcc
     2041 cagcccgcgc cgagccgcac cacccccgag gaaaccgacg aggccgccga gcgcgccaac
     2101 atcgccgtgg tgccgctgac gatcccgctg ctgaccgggc cgggcacggt cagcacggtg
     2161 atcatctacg ccgaccaggg tacgcgaacg gtgctgatgc tgctcggcgc cggcctcgtg
     2221 atgggcgtcg tggtgtggtt cgccttcaac ctggccggtc cgatcagccg cctggccggg
     2281 cgcaccggac tcaacatcat gacccggctg atgggcctgg tgctggcggc gctcgcggtg
     2341 gagttcatcg cagcgggcat ccgtgcctat gtgaacgcct gagggcgcc
//
LOCUS       DAOQNN010000473         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_35176_length_2385_cov_12.038627, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000473 DAOQNN010000000
VERSION     DAOQNN010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35176_length_2385_cov_12.038627"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /gene="rpsL"
                     /locus_tag="PKV97_16305"
     CDS             complement(<1..179)
                     /gene="rpsL"
                     /locus_tag="PKV97_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="interacts with and stabilizes bases of the 16S rRNA
                     that are involved in tRNA selection in the A site and with
                     the mRNA backbone; located at the interface of the 30S and
                     50S subunits, it traverses the body of the 30S subunit
                     contacting proteins on the other side; mutations in the
                     S12 gene confer streptomycin resistance; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNB07496.1"
                     /translation="MPTINQLVRKPRQLEVAKSKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNG"
     gene            complement(314..>2385)
                     /locus_tag="PKV97_16310"
     CDS             complement(314..>2385)
                     /locus_tag="PKV97_16310"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355315.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNB07497.1"
                     /translation="VDQESFNAIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIET
                     PITTNFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVTQDLVVIEDDCG
                     TREGFNMKALIEGGEVVEPLRERILGRVCVEDIVNPESQETMIEAGSLLDEDAVDLIE
                     SLGVDEVKVRTPLTCETRYGLCAQCYGRDLGRGSRVNVGEAVGVIAAQSIGEPGTQLT
                     MRTFHVGGAASRAAAASGVEAKSAGSIRFAGNMRYVSNAKGEKIVIARSAELVVADDM
                     GRERERHKLPYGAMLLVDDAAPVKAGTQLASWDPHTRPIVTEYTGTVKFENVEEGVTV
                     AKQIDEVTGLSTLVVIDAKRRSSTGSPKGVRPQVKLLDENGEEVKIAGTEHSVAITFQ
                     VGSLITVKDGQQINVGDILARIPQESAKTRDITGGLPRVAELFEARAPKDAGMLAEYT
                     GTVSFGKETKGKQRLVITEPDGTSHEFLIPKDKHLMVHDGQVVNKGELIVDGPSDPHD
                     ILRLQGISELARYIIDEVQDVYRLQGVKINDKHIEVIVRQMLRRVVITDPGDTRFIRE
                     EQVERSEVLDENDRVEAEGKLPAQYENVLLGITKASLSTDSFISAASFQETTRVLTEA
                     AIMGKRDELRGLKENVIVGRLIPAGTGLAYHRSRKAQEEGRDLGAGHAWAPAEEVVET
                     QQDIEAS"
BASE COUNT          367 a          792 c          771 g          455 t
ORIGIN      
        1 ccgttggtca ggcgcacctt ggcgacctta cgcagcgccg agttcggctt cttcggggtc
       61 gtggtgtaga cgcgggtgca aacgccgcgc ttctgcggac aagcctcgag tgccggaacc
      121 ttgctcttgg caacttccag ttggcgcggc ttgcgaacaa gctgattgat tgttggcata
      181 gcttaagaat ttcccataag accgcggcag cccaccatgg gccgccgcgg gcggattatt
      241 ccggctgaga ctgactacga ttggtcagcc cacaaaaaag aggccggagt ttagtccgag
      301 actgtccgaa aagtcaactc gcctcgatgt cctgctgtgt ttccacaacc tcttccgccg
      361 gcgcccaggc atgccccgca ccgagatcgc gcccctcttc ctgcgccttg cggctgcggt
      421 ggtaggccag accggtaccg gccgggatca ggcgaccgac gatgacgttc tccttcaggc
      481 cgcgcagttc gtcgcgcttg cccatgatcg ccgcctcggt cagcacgcgg gtggtctcct
      541 ggaaggaggc cgcggagatg aaggagtcgg tcgacagcga ggccttggtg atgcccagca
      601 gcacgttctc gtactgcgcc ggcagcttgc cttcggcttc gacgcggtcg ttctcgtcca
      661 gcacctcgga gcgctcgacc tgctcctcgc ggatgaaccg ggtatcgccc ggatcggtga
      721 tgaccacgcg acgcagcatc tggcggacga tcacctcgat gtgcttgtcg ttgatcttca
      781 cgccctgcag gcgatacacg tcctgcacct cgtcgatgat gtagcgcgcg agttcgctga
      841 tgccctgcag gcgcaggatg tcgtgcggat cggacggacc atccacgatc agttcgccct
      901 tgttaaccac ctggccgtcg tgcaccatca ggtgcttgtc cttggggatc aggaactcgt
      961 gcgaggtccc gtccggctcg gtgatcacca gacgctgctt gcccttggtc tccttgccga
     1021 aggacaccgt gccggtgtac tcggcgagca tgccggcatc cttgggcgca cgcgcctcga
     1081 agagctcggc cacgcgcggc aggccgccgg tgatgtcgcg ggtcttcgcc gactcctgcg
     1141 ggatgcgcgc gaggatgtcg ccgacgttga tctgctggcc gtccttcacg gtgatcaggg
     1201 agccgacctg gaaggtgatc gcaaccgagt gctcggtgcc ggcgatcttg acctcctcgc
     1261 cgttctcgtc cagcagcttg acctgcggac ggacgccctt ggggctgccg gtggacgagc
     1321 gacgcttggc atcgatgacc accagcgtgg acagaccggt aacttcgtcg atctgcttgg
     1381 cgacggtgac gccctcctcc acgttctcga acttcaccgt accggtgtat tcggtcacga
     1441 tcgggcgggt gtgcggatcc cacgaggcga gctgcgtgcc agccttgacc ggagcagcgt
     1501 catcgaccag cagcatggcc ccgtagggca gcttgtggcg ctcgcgctcg cggcccatgt
     1561 cgtcggccac cacgagctcg gccgagcgcg cgatgacgat cttttcgccc ttggcgttcg
     1621 agacgtagcg catgttgccg gcaaagcgga tcgaacctgc cgacttggcc tcgacgccac
     1681 tcgcggcggc ggctcgcgaa gccgcccccc cgacgtggaa ggtccgcatg gtcagctgcg
     1741 tgcccggctc gccgatcgac tgcgccgcga tgacgccgac ggcctcgccg acgttgaccc
     1801 gggaaccacg acccagatcg cggccgtagc actgcgcgca caggccgtag cgggtttcgc
     1861 aggtgagcgg cgtgcgcacc ttgacctcgt ccacgcccag gctctcgatc aggtccaccg
     1921 cgtcctcgtc gagcaggctt cccgcctcga tcatggtttc ctggctttcc ggattgacga
     1981 tgtcctcgac gcagacgcgg cccaggatgc gctcgcgcag cggctccacg acctcgccgc
     2041 cctcgatgag ggccttcatg ttgaagcctt cgcgcgtgcc gcagtcgtcc tcgatgacca
     2101 cgagatcctg ggtcacgtcg acgagacggc gagtcaggta gcccgagttc gcggtcttca
     2161 gcgcggtgtc ggccaggccc ttacgggcgc cgtgcgtcga gatgaagtac tgcaggacgt
     2221 tgaggccttc acggaagttg gtcgtgatcg gggtctcgat gatggaaccg tccggtttcg
     2281 ccatcaggcc gcgcatgccg gcgagctgcc ggatctgcgc cgcggaacca cgcgcgcccg
     2341 agtcggccat catgtagatc gcgttgaagg actcctggtc gacgt
//
LOCUS       DAOQNN010000474         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_35248_length_2382_cov_10.855608, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000474 DAOQNN010000000
VERSION     DAOQNN010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35248_length_2382_cov_10.855608"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PKV97_16315"
     CDS             <1..1024
                     /locus_tag="PKV97_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HNB07498.1"
                     /translation="EALERARPPPRHAEAHPGASASESATARNNFLALMFVLMVGTAA
                     LPHILMRYYTTPGVMEARRSVLWSLFFIFLLYVTAPAYAVFAKWEVYNNLVGSNIAIL
                     PDWVASWGRVGLVRIEDLNGDGILQLAELRLNTDVIVLATPEIAGLPYVVSGLVAAGG
                     LAAALSTADGLLLTISNALSHDLYYKVINPKASAHRRLVISKSQLLVVAVVAAWVASM
                     RPDNILFMVGLAFSIGGSAFFPALVLGIFWKRANRPGAVAGMLAGLAVTVFYVVRTHP
                     FFGGAMANAWFEINPISAGVFGVPLGFATIVAVSLLTKPPPQEIQDLVDYVRYPDIPG
                     QSRPHL"
     gene            1095..>2382
                     /locus_tag="PKV97_16320"
     CDS             1095..>2382
                     /locus_tag="PKV97_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNB07499.1"
                     /translation="MRFDKLTTKFQQALADAQSIAVGNDQQFIEPLHVLQALLEQDDG
                     GSVSLLQRAGVNVPPLKNAVAKAVGRLPKVEGHGGEVSIGRDLNNLLNVTDREAQKRG
                     DQFIASEMFLLALADDKGEAGRLLKENGLARKALEAAIEAVRGGANVGSQDAEGQRES
                     LSKYCLDLTERARSGKLDPVIGRDDEIRRAIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNDEVPETLKGKRVLSLDMAALLAGAKYRGEFEERLKAVLKEIAQEEGRIIVF
                     IDELHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRKYIEKDAALERRFQ
                     KVLVEEPSVEATIAILRGLQEKYEIHHGVDITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARIKMEIDSKPEVMDKLDRRMIQLKIEREAV"
BASE COUNT          408 a          827 c          788 g          359 t
ORIGIN      
        1 cgaggcgctc gaacgcgccc gcccgccgcc gcgccacgcc gaggcgcacc cgggcgccag
       61 cgccagcgag tccgccaccg cgcgcaacaa ctttctcgcg ctgatgttcg tgctcatggt
      121 gggcacggcg gcgctgccgc acatcctcat gcgctactac accacgcccg gcgtcatgga
      181 ggcgcggcgc tcggtgctgt ggtcgctgtt cttcatcttc ctgctctacg tcaccgcgcc
      241 ggcctacgcg gtgttcgcca agtgggaggt ctataacaat ctggtcggct cgaacatcgc
      301 gatcctgccc gactgggtcg cgtcgtgggg cagggtgggg ctggtgcgca tcgaggacct
      361 caacggcgac ggcatcctgc aactcgccga gctgcgcctg aacaccgacg tgatcgtgct
      421 cgccaccccg gagatcgccg gcctgcccta cgtggtatcg ggcctggtcg cggccggggg
      481 gctggccgcc gcgctatcga ccgccgacgg ccttttgctg acgatctcga acgcgctgtc
      541 gcacgatctc tactacaagg tcatcaaccc caaggcgagc gcgcaccggc gcctggtgat
      601 ctccaagtcg cagctgctgg tggtggcggt ggtggcggcc tgggtggcct cgatgcggcc
      661 ggacaacatc ctcttcatgg tcggcctggc cttctcgatc ggtggctcgg ccttcttccc
      721 ggcgctcgtg ctgggcatct tctggaagcg cgccaaccgc ccgggggcgg tcgccggcat
      781 gctggccggg ctggcggtca cggtgttcta cgtggtgcgt acccatccct tcttcggcgg
      841 cgcgatggcg aacgcctggt tcgaaatcaa cccgatctcg gccggcgtgt tcggcgtgcc
      901 gctcggcttt gcgaccatcg tcgcggtcag cctgctgacc aagccaccgc cgcaggagat
      961 ccaggacctg gtcgactacg tacgttaccc cgacatcccg ggacaaagcc ggccgcatct
     1021 ttgagcgcgc acttgaagcg cgccggatgc gccccacata gggttcagtt caacgaatcc
     1081 cgaggatgac ctccatgcgc ttcgacaagc tcaccaccaa gttccagcag gccctcgccg
     1141 atgcgcagag catcgccgtg ggcaacgacc agcagttcat cgagccgctg cacgtgctgc
     1201 aggccctgct cgagcaggac gacggcggca gtgtgtcgct gctgcagcgc gccggggtca
     1261 atgtcccgcc gctcaagaat gcggtcgcca aggccgtcgg ccgcctgccc aaggtggaag
     1321 ggcacggcgg cgaggtcagc atcgggcgcg acctcaacaa cctgctcaac gtcaccgacc
     1381 gcgaggcgca gaaacgcggc gaccagttca tcgccagcga gatgttcctg ctcgcgctcg
     1441 cggacgacaa gggcgaggcc ggccgcctgc tcaaggagaa cggcctggcg cgcaaggcgc
     1501 tcgaggccgc gatcgaggcg gtgcgcggcg gcgccaacgt gggcagccag gacgccgaag
     1561 gccagcgcga atcgctgtcg aagtactgtc tcgacctcac cgagcgcgca cgaagcggca
     1621 agctcgaccc ggtgatcggt cgtgacgacg agatccgccg cgccatccag atcctgcagc
     1681 gccgcaccaa gaacaacccg gtgctgatcg gcgagccggg cgtgggcaag accgcgatcg
     1741 tcgagggcct ggcgcagcgc atcgtcaatg acgaggtgcc cgagacgctc aagggcaagc
     1801 gcgtgctctc gctcgacatg gcggcgctgc tcgccggcgc caaataccgc ggcgagttcg
     1861 aggagcgcct gaaggcggtg ctcaaggaga tcgcccagga agaggggcgc atcatcgtct
     1921 tcatcgacga gctccacacc atggtgggcg ccggcaaggc cgagggcgcg atcgatgccg
     1981 gcaacatgct caagcccgcg ctcgcacgcg gcgagttgca ctgcatcggc gccaccacgc
     2041 tcgacgaata ccgcaagtac atcgagaagg acgcggcgct cgagcgccgc ttccagaagg
     2101 tgctcgtcga ggagccttcg gtcgaggcga ccatcgccat cctgcgcggc ctgcaggaga
     2161 agtacgagat ccaccacggc gtcgacatca ccgacccggc catcgtcgcc gcggccgaac
     2221 tgtcgcaccg ctatatcacc gaccgcttcc tgcccgacaa ggcgatcgac ctgatcgacg
     2281 aggccgcggc gcgcatcaag atggagatcg actccaagcc cgaggtcatg gacaagctcg
     2341 accgtcgcat gatccagctc aagatcgagc gcgaggcggt ga
//
LOCUS       DAOQNN010000475         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_35557_length_2369_cov_7.104149, whole genome shotgun sequence.
ACCESSION   DAOQNN010000475 DAOQNN010000000
VERSION     DAOQNN010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35557_length_2369_cov_7.104149"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1410
                     /locus_tag="PKV97_16325"
     CDS             7..1410
                     /locus_tag="PKV97_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB07500.1"
                     /translation="MTAEPPSLERSPTRPRRLHLLRTAAFVLGAGAAAGALAAPEIVE
                     WTAPTGARVHFVESRALPLVDIQISFAAGGALDPEGKAGLASMTQALLDAGAGGLDEQ
                     TIADRKADLGIELSGGTDNDRASMALRSLSSPAELDAAVELTATLLARPDFPAAVLER
                     ERSRAIAGLREALTKPATLAARQFNAALYTGHPYGHDSTPESLAAIGRDDLVDFHRRH
                     YGANRATVAIVGDVDRAAAERIAVRLTAGLPATQAAMPLPAPAPTTARTLRIPHPSAQ
                     AHILVGQPGMAREDADYFPLLVGNYVLGGGGFVSRLTAEVREKRGFAYSVYSALSPQQ
                     VAGPFQIGLQTRGSQAEEALAVVRATLAGFLADGPSAEELQAAKDNLINGFGLRLDSN
                     AKLLDYVSMIGFYRLPLDWLERYPQQVAAVDVAAVRAAWQRRIRPEQLVTVIAGGDGD
                     RPAAAGASTTPPAEAAQ"
     gene            1407..1946
                     /gene="rsmD"
                     /locus_tag="PKV97_16330"
     CDS             1407..1946
                     /gene="rsmD"
                     /locus_tag="PKV97_16330"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNB07501.1"
                     /translation="MSRVRIVGGQWRSRLLDVASVPGLRPTPDRVRETLFNWLGQDLD
                     GQACLDLFAGTGILGFEAASRGAARVVLVEHDARALDALHKAAKTLQAAQVEIVRGDA
                     VRFAQSTPQRFDGVFLDPPYKQGWIERVSPWLDRLVRPEGWLYVESEVAVDSLGAWRA
                     VKQGRAGQVHFHLMRRSEE"
     gene            1943..>2369
                     /gene="coaD"
                     /locus_tag="PKV97_16335"
     CDS             1943..>2369
                     /gene="coaD"
                     /locus_tag="PKV97_16335"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNB07502.1"
                     /translation="MKEAIAVYPGTFDPFTRGHEDLVRRASILFEKVVVAVARSNSKN
                     PIFGLEDRVDIARDAVSAFPNVEVVGFDCLLMEFLQQQDARVIIRGLRAVSDFEYEFQ
                     MAGMNRKLYPDVETVFLTPGEEFMFISATMVREIARLGGD"
BASE COUNT          340 a          910 c          782 g          337 t
ORIGIN      
        1 cccgccatga ccgcagaacc ccccagcctc gagcgctccc ccacccgccc ccgccgcctc
       61 catctcctgc gcaccgccgc gttcgtactt ggtgccggcg cggcggccgg cgcgctcgcc
      121 gcccccgaga tcgtcgaatg gaccgcgccc accggcgcgc gggtgcactt cgtcgagagc
      181 cgcgcgctgc cgctggtcga catccagatc tccttcgctg ccggcggcgc gctcgacccc
      241 gagggcaagg ccgggctggc gagcatgacc caggccctgc tcgacgccgg cgccggcggg
      301 ctcgacgagc agaccatcgc cgaccgcaag gccgatctcg gcatcgagct ctccggcggc
      361 accgacaacg accgcgccag catggcgctg cgcagcctgt cctcgcccgc cgagctcgac
      421 gccgcagtcg agctcaccgc caccctgctc gcccgtcccg actttcccgc cgcggtgctc
      481 gagcgcgagc gcagccgcgc gatcgccggc ctgcgcgagg cgctgaccaa gcccgccacg
      541 ctcgccgcgc gccagttcaa cgccgcgctc tacaccggcc acccttatgg ccacgacagc
      601 acgccggagt cgctcgccgc gatcgggcgc gacgacctgg tcgacttcca tcgccgccac
      661 tacggtgcca accgcgccac ggtggcgatc gtcggcgacg tggaccgcgc cgccgccgag
      721 cgcatcgcgg tccgcctcac cgcgggcctg cccgcgacgc aggccgccat gccgctgccg
      781 gcgcccgcgc ccaccacggc ccggaccttg cgcatcccgc acccctcggc gcaggcccac
      841 atcctcgtcg gccagcccgg catggcgcgc gaggacgccg actacttccc cttgctggtg
      901 ggcaactacg tgctcggcgg tggcggcttc gtgtcgcggc tgaccgccga ggtacgcgag
      961 aagcgcggtt ttgcctacag cgtgtacagc gcgctgtcgc cacagcaggt cgccggcccc
     1021 ttccagatcg gcctgcagac gcgcggaagc caggccgagg aagcgctcgc ggtggtgcgg
     1081 gcgacgctcg cgggcttcct cgccgacggc cccagcgccg aggagttgca ggccgccaag
     1141 gacaacctga tcaatggctt cggcctgcgt ctcgactcca acgccaagct gctcgactac
     1201 gtgtcgatga tcggcttcta ccgcctgccg ctcgactggc tggagcgcta cccgcagcag
     1261 gtcgccgcgg ttgacgtcgc cgcggtgcgc gcggcctggc aacgccgcat ccgcccggaa
     1321 cagctcgtca ccgtgatcgc cggcggcgac ggcgaccgtc ccgcggcggc gggtgcgtcc
     1381 accacgcccc ccgcggaagc cgcgcagtga gccgggtccg catcgtcggc ggacagtggc
     1441 gctcgcgcct gctcgacgtg gcctcggtgc caggcctgcg cccgacgccc gaccgcgtgc
     1501 gcgagaccct gttcaactgg ctgggccagg acctcgacgg ccaggcctgc ctggacctct
     1561 tcgccggcac cggcatcctc ggcttcgagg ccgcctcacg cggcgccgcc cgcgtcgtcc
     1621 tggtcgagca cgacgcgcgc gcgctcgacg cactgcacaa ggcggcaaag accctacagg
     1681 cggcgcaagt agagatcgtg cgcggcgatg cggtaagatt cgcgcaatcc acgccgcagc
     1741 gtttcgacgg cgtgttcctc gatccgccct acaagcaggg ctggatcgag cgggtgtcgc
     1801 cttggctgga ccggctggtc cgtcccgagg gatggctcta tgtcgaatcc gaagtcgccg
     1861 tggacagcct cggcgcatgg cgcgcggtca agcagggccg cgccgggcag gtccatttcc
     1921 atctcatgcg caggagcgag gaatgaagga agcgatcgcg gtctatccgg gcaccttcga
     1981 cccgttcacc cgcggccacg aggatctcgt gcggcgcgcc tcgatcctgt tcgagaaggt
     2041 cgtcgtcgcg gtcgcgcgca gcaacagcaa gaacccgatc ttcggactgg aggaccgggt
     2101 cgacatcgca cgcgatgccg tctcagcctt tcccaacgtc gaagtggtcg gcttcgactg
     2161 cctgctgatg gagttcctcc agcagcagga tgcgcgcgtg atcatccgcg ggctgcgtgc
     2221 ggtatccgac ttcgaatacg aattccagat ggcagggatg aaccgcaagc tttatccgga
     2281 cgtcgagacg gtgttcctga cgcccggtga agaattcatg ttcatctccg ccacgatggt
     2341 gcgggagatc gcccgcctcg gcggcgacg
//
LOCUS       DAOQNN010000476         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_35721_length_2363_cov_5.979636, whole genome shotgun sequence.
ACCESSION   DAOQNN010000476 DAOQNN010000000
VERSION     DAOQNN010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35721_length_2363_cov_5.979636"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..906
                     /locus_tag="PKV97_16340"
     CDS             28..906
                     /locus_tag="PKV97_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNB07503.1"
                     /translation="MAGDPRSEVTAAATEIPPMPDRELTSQPEPEPEPIAQAAVIAAP
                     DPDAERLLARCAQLEEQMVELSRRLADMEQQVRSYEQHQYAALGEVVGECLRLRQEYL
                     RLKAERSGRAADAERARQADEDFDAYRRSTEHTAAPLPELDADEQDELRRLYRAAAMR
                     CHPDRAAESERAAAHDIFLRVQAVYQARDLEGLRRLAAELAGWVETTPGAAAAGAGSL
                     KAPAGSGIRRRVGELQHKVADLILAVQTLQLDPIYRKARQVEHWDTHFGDARAGFEAE
                     CEALRAGIAGLASRSA"
     gene            complement(918..2294)
                     /gene="dbpA"
                     /locus_tag="PKV97_16345"
     CDS             complement(918..2294)
                     /gene="dbpA"
                     /locus_tag="PKV97_16345"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HNB07504.1"
                     /translation="MPFDGLPLSPAMLDTLRQLDYRAMTPIQAASLPLALAGHDLIAQ
                     AKTGSGKTAAFALPLLARLDVRKLAVQAMVLCPTRELADQVTQEIRRLARFEANIKVL
                     ALCGGTPMRPQRDSLEHGAHVVVGTPGRIMDHLERGSLVLEALNTLVLDEADRMLDMG
                     FHDDIAFVASRCPSERQTLLFSATYPEGIARLAARFLRKPQEVKLLEQHAHGKIRQRF
                     YEVAHPARLEAVATLLRHFRPVSTLAFCNTRQQCRDLVELLRARGFAALALNGEMEQR
                     ERDQVLIQFANRSCSVLVATDVAARGLDIARLEAVINVDVTPDPEIHIHRIGRTGRGD
                     EEGWALNLCSSADKRRVASIAQRMGFEPEWHALDELVEDANEPLQAPMTTLLILGGRK
                     DKIRPADVLGALTGEAGFTREQVGKITVTDQTTYVAVERAIAHEAVRRLAAGKLKGRK
                     VKVRALEI"
BASE COUNT          325 a          794 c          907 g          337 t
ORIGIN      
        1 gcggggccgt ccgcagtgcc gcccgaggtg gccggcgatc cgcggtccga agtcacggct
       61 gcagcgacgg agatcccgcc gatgccggat cgtgagctca cgtcccagcc ggagcctgag
      121 cctgagccga tcgcgcaggc ggcggtcatc gccgcgcccg atccggacgc ggagcgcctg
      181 ctggcgcgct gcgcccagct ggaggaacag atggtcgagc tcagccggcg tctggctgac
      241 atggagcagc aggtgcgcag ctacgagcag caccagtacg ctgccctcgg cgaagtggtg
      301 ggcgagtgcc tgcgcctgcg ccaggagtac ctcaggctca aggctgagcg ctcgggcagg
      361 gcggccgatg ccgaaagggc ccgccaggcc gacgaggact tcgacgccta ccgtcgcagc
      421 accgagcaca ccgccgcccc attgcccgag ctcgacgcgg acgaacagga cgaactgcgc
      481 cggctgtatc gcgccgcggc catgcgttgc catccggacc gcgccgcgga gagcgagcgc
      541 gccgccgcgc acgacatctt cctgcgcgtg caggccgtct accaggcccg cgacctcgag
      601 ggcctgcgcc gcctcgccgc ggagctggcg gggtgggtgg agacgacgcc gggggcagcc
      661 gcggcagggg cgggtagctt gaaggcgccc gcgggcagcg gcatcaggcg ccgcgttggc
      721 gagctgcagc acaaggtcgc cgacctgatc ctcgccgtgc agacgctgca gctcgatccc
      781 atttaccgca aggcacgaca ggtcgagcac tgggacacgc attttggcga cgcgcgtgca
      841 ggcttcgagg ccgaatgcga agccttgcgt gccgggattg cgggactggc ctcccgttcg
      901 gcctgaggct gcgcgcctca tatctccagc gcgcgcacct tcaccttccg gcccttgagc
      961 ttgccggccg caaggcgacg caccgcctcg tgtgcgatcg cgcgctcgac cgcgacgtag
     1021 gtggtctggt cggtgacggt gatcttgccg acctgctcgc gggtgaagcc ggcctcgccg
     1081 gtcagcgcgc cgagcacgtc cgcagggcgg atcttgtcct tgcgtccgcc caggatcagc
     1141 agcgtcgtca tcggcgcctg caggggctcg ttcgcgtctt ccaccagttc gtcgagggcg
     1201 tgccactcgg gctcgaagcc catcctctgc gcgatgctcg cgacgcggcg cttgtccgcg
     1261 ctgctgcaca ggttcagcgc ccagccctct tcgtcgccgc ggccggtgcg gccgatgcgg
     1321 tggatgtgga tctccgggtc gggggtgacg tcgacgttga tcaccgcctc gagacgtgcg
     1381 atgtccagcc cgcgcgcggc gacgtcggtg gcgacgagca ccgagcagct gcggttggcg
     1441 aactggatca gcacctggtc acgctcgcgc tgctccatct cgccgttgag ggcgagcgcc
     1501 gcgaagcccc gcgcgcgcag cagctcgacg aggtcgcggc actgctggcg ggtgttgcag
     1561 aaggccagcg tgctgaccgg gcggaagtgg cgcagcagcg tcgcgaccgc ctccagccgc
     1621 gccgggtggg cgacctcgta gaaacgctgg cggatcttgc cgtgcgcgtg ttgctcgagc
     1681 agcttcacct cctgcggctt gcgcaggaaa cgcgcggcca ggcgggcgat gccctcgggg
     1741 taggtggcgg agaacagcag ggtctggcgc tcggaggggc agcggctggc cacgaaggcg
     1801 atgtcgtcgt ggaaacccat gtccagcatg cggtcggctt cgtccagcac cagcgtgttc
     1861 agggcctcga gcaccaggct gccgcgttcg aggtggtcca tgatgcgccc gggcgtgccg
     1921 accacgacgt gggcgccgtg ctcgaggctg tcgcgctgcg ggcgcatcgg cgtgccgccg
     1981 cacagcgcca gcaccttgat gttggcctcg aagcgcgcca ggcggcggat ctcctgggtg
     2041 acctggtcgg ccagttcgcg ggtcgggcac agcaccatgg cctggacggc gagcttgcgc
     2101 acgtcgagcc gggccagcag gggcagggcg aaggccgcgg tcttgccgct gccggtcttg
     2161 gcctgggcga tgaggtcgtg cccggcgagg gccagcggca ggctggccgc ctggatcggg
     2221 gtcatggccc ggtagtcgag ctggcgcagg gtgtccagca tggcgggcga gagcggcagg
     2281 ccgtcgaaag gcaggccggg ggttgcgggc gggagtgacc cgggcggggc gtccggggga
     2341 ctcgcgagga tgggggagga ggg
//
LOCUS       DAOQNN010000477         2354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_35901_length_2354_cov_10.999565, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000477 DAOQNN010000000
VERSION     DAOQNN010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35901_length_2354_cov_10.999565"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1592)
                     /gene="pntB"
                     /locus_tag="PKV97_16350"
     CDS             complement(177..1592)
                     /gene="pntB"
                     /locus_tag="PKV97_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HNB07505.1"
                     /translation="MTSSLATVSYIGATILFILSLGGLAHPETARRGNLFGIAGMSIA
                     VLATVFGPQVGMGGIPWIVIALVIGAAVGLYAAKKVQMTQMPELVALMHSLVGLAACL
                     VGFASYVDTSLVFPTAAEKTIHEVEIYVGILIGAVTFSGSLVAFAKLSGKIGGKPLLL
                     PARHWLNLAGLILVILLGRWFLNTHSIWAGMALLLVMTLIALAFGVHMVMAIGGADMP
                     VVVSMLNSYSGWAAAATGFMLGNDLLIVVGALVGSSGAILSYIMCRAMNRNFVSVIAG
                     GFGSGSGAPAATADGSEPAGEVTSISAVDTADLLRDAKNIVIVPGYGMAVAQAQHVVC
                     EIVNVLRGQGVAVRFAIHPVAGRMPGHMNVLLAEAKVPYDIVLEMDEINADFPETDVA
                     IVIGANDIVNPAAQEDPGSPIAGMPVLEVWKARTSIVMKRSMASGYAGVDNPLFYKEN
                     NRMLFGDARKMLDEVLGALSA"
     gene            complement(1603..>2354)
                     /locus_tag="PKV97_16355"
     CDS             complement(1603..>2354)
                     /locus_tag="PKV97_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HNB07506.1"
                     /translation="KPGSVIVDLAAEQGGNCELTVPGEAVVRHGVTIIGYTDLVSRLA
                     RQSSTLYSNNLLRLTEDLCKAKDGNIAVNFEDDAIRGLTVIKQGELTWPAPPPKLPAA
                     SAKPKASSAPITAPKGHGHGAGAPMPVKSLAIVFGVGALLFLLVGLYAPASFLTHFTV
                     FVLACFVGYMVVWNVTPSLHTPLMSVTNAISSIIAIGALVQIAPPLAEGASDRPGGLI
                     GVLAFVAITLTAVNMFGGFAVTRRMLAMFRK"
BASE COUNT          405 a          767 c          808 g          374 t
ORIGIN      
        1 agcgcgacac gaaatctgca ctgcagcgcg cccggatctg actctccagg ttggccgtac
       61 gattgtccgg gcaggatcca cgtcccgatt gcagccaccg cggctcaggt cgggcgggct
      121 tcaccgcggt gccgccgcca cgcgaaaccc cggcacgcgg atccgcccgt gctgcttcaa
      181 gcgctcagcg cgcccaacac ctcgtcgagc atcttgcggg cgtcgccgaa cagcatgcgg
      241 ttgttttcct tgtagaacag cgggttgtcg accccggcgt agccggaagc catgctgcgc
      301 ttcatgacga tcgaggtccg tgccttccac acctcgagca ccggcatgcc ggcgatcggg
      361 ctgcccgggt cttcctgcgc ggccgggttc acgatgtcgt tggcaccgat cacgatcgcg
      421 acatccgttt cggggaagtc cgcgttgatc tcgtccatct cgagcacgat gtcgtaggga
      481 accttggcct cggccagcag gacgttcatg tggccgggca tgcggcctgc caccgggtgg
      541 atggcgaagc gcacggcgac gccctgtccg cgcagcacgt tgacgatctc gcagacgacg
      601 tgctgtgcct gtgcgaccgc catgccgtag cccggcacga tgacgatgtt cttcgcgtcg
      661 cgcagcaggt cggcggtatc caccgcgctg atcgacgtga cctcgccggc cggctccgat
      721 ccgtcggccg tcgccgcggg cgcgccgctg ccggagccga agccgcccgc gatgacgctg
      781 acgaagttgc ggttcatcgc gcggcacatg atgtaggaca ggatcgcccc cgaggagccc
      841 accagcgcgc cgacgacgat cagcagatcg ttgcccagca tgaagcccgt cgccgccgcc
      901 gcccaacccg aatagctgtt gagcatcgag accacgaccg gcatgtcggc gccaccgatc
      961 gccatcacca tgtgcacgcc gaaggccagc gcgatcaacg tcatcaccag cagcaaggcc
     1021 atgccggccc agatcgagtg tgtgttgagg aaccagcgcc cgagcaggat caccaggatc
     1081 agcccggcga ggttgagcca gtggcgcgcg ggcagcagca gcggcttgcc gccgatcttg
     1141 ccggagagct tggcgaaggc gaccagcgag ccggagaagg tgactgcgcc gatcaggatg
     1201 ccgacgtaga tctccacctc gtggatggtc ttctcggcag cggtggggaa caccagggag
     1261 gtgtccacgt agctcgcgaa gcccaccagg caggccgcca gacccaccag gctgtgcatg
     1321 agggcgacga gctcgggcat ctgcgtcatc tgcaccttct tcgcggcgta gaggccgacg
     1381 gcggcgccga tgacgagggc gatgacgatc catggaatgc cgcccatgcc gacttgcggg
     1441 ccgaacacgg tggccagcac cgcgatgctc atgcccgcga tgccgaacag gttgccgcgg
     1501 cgcgcggtct cgggatgggc gagtccgccc aggctgagga tgaagaggat ggtggcgccg
     1561 atgtaagaga cagtggccag acttgaggtc attgtcttct ccttatttgc ggaacatcgc
     1621 cagcatgcga cgcgtcaccg cgaagccgcc gaacatgttg acggccgtga gggtgatggc
     1681 gacgaaggcg agcacgccga tcagcccccc cggtcgatcg ctggcgcctt cggccagcgg
     1741 tggggcgatc tgcacgagcg cgccgatcgc gatgatcgac gagatggcgt tggtgacgct
     1801 catcagcggc gtgtgcagcg agggtgtgac gttccagacc accatgtagc ccacgaagca
     1861 ggccagcacg aacacggtga agtgcgtgag gaagctggcc ggggcgtaca ggccgacgag
     1921 caggaacagc agcgcgccga caccgaacac gatcgccagc gacttgaccg gcatcggcgc
     1981 gccggccccg tggccatggc ccttgggtgc ggtgatcggt gcgctcgaag ccttgggttt
     2041 ggccgatgcg gccggcagct tgggaggtgg tgcgggccag gtgagctcac cctgcttgat
     2101 cacggtgagg ccacggatcg cgtcgtcctc gaagttgacg gcgatgttgc catccttcgc
     2161 cttgcacagg tcttcggtga ggcggaggag gttgttcgaa tacagggtcg aggattgcct
     2221 ggccagccgg ctcacgaggt cggtgtagcc gatgatcgtg actccatggc gtaccaccgc
     2281 ctcgccgggc acggtgagct cgcagttgcc gccctgctcg gcggcgaggt cgacgatcac
     2341 gctgcccggc ttca
//
LOCUS       DAOQNN010000478         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_36182_length_2344_cov_6.417213, whole genome shotgun sequence.
ACCESSION   DAOQNN010000478 DAOQNN010000000
VERSION     DAOQNN010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36182_length_2344_cov_6.417213"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2344
                     /locus_tag="PKV97_16360"
     CDS             <1..>2344
                     /locus_tag="PKV97_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNB07507.1"
                     /translation="ATQPTEPAPAEGPQVITADGRCVPLPLEQLEAAVARACAGLAHA
                     EPAPILTELRRNLYDGMPMAEVGQALVMSARTLIERDPDYSRVAARLLLDVLRREAAE
                     VLALAPDGEAQTPQAWYADYFRSYLARGVQAQRLDPRLLEFDLDTLSAALEPARDLDF
                     TYLGLQTLYDRYLLDLDGRRFELPQAFFMRVAMGLALNEVDREARAIEFYRLLSSFEF
                     MSSTPTLFNAGTLRPQLSSCYLTTVPDDLDGIFGAIRDNALLSKFAGGLGNDWTRVRG
                     LGALIKGTHGKSQGVVPFLKVANDTAVAVNQGGKRKGAVCAYLETWHIDIEEFLELRK
                     NTGDERRRTHDMNTAHWIPDLFMRRVCEEADWTLFSPDETPDLHDLVGAAFEARYLAY
                     EQLAAAGGLRHYKRIPAVQLWRKMLSMLFETGHPWLTFKDACNLRYTNAHLGRVHSSN
                     LCTEITLHTSDEEIAVCNLGSINLAAHVDAGVLDEDKLVRTITTAVRMLDNVIDINWY
                     SVPQARRSNLRHRPVGLGLMGFQDALHRMQLAYASPAAVEFADRSMELISYHAIAASS
                     ALAAERGRYPSYEGSLWSQGILPIDSLALLAEARGETLEVDRSATLDWASLREQVRRQ
                     GMRNGNVLAIAPTATIANICGVSASIEPTFQNLFVKSNLSGEFTVANPYLVRELQALG
                     LWDPVMINDLKYYDGSLGPIARVPAELKARYACAFEIDPVWLVEAAARRQKWLDQAQS
                     LNLYVAEASGAQLDALYKLAWRRGLKTTYYLRTRGATHLEK"
BASE COUNT          381 a          815 c          757 g          391 t
ORIGIN      
        1 gccacgcagc ccaccgagcc cgcacctgcc gaggggccgc aggtcatcac cgccgatggg
       61 cgctgcgtgc ccttgccgct ggaacaactc gaagccgccg ttgcccgcgc ctgcgcgggg
      121 ctcgcccatg ccgagccggc gccgatcctg accgagctgc gccgcaatct ctacgacggc
      181 atgccgatgg cggaggtggg gcaggcgctg gtgatgagcg cgcgcacctt gatcgagcgc
      241 gatcccgatt acagccgcgt cgccgcccgc ctgctgctcg atgtcttgcg ccgcgaggcg
      301 gccgaggtgc tggcgctggc gcctgatggc gaggcacaga caccgcaggc ctggtacgcc
      361 gactatttcc gcagctatct ggcgcgtggc gtgcaggcgc agcgcctgga cccgcgcctg
      421 ctcgagttcg acctcgacac cttgagcgcg gcgctcgagc cggcgcgcga tctggacttc
      481 acctatctgg gcctgcagac gctctatgac cgctatctgc tcgacctcga cgggcgccgc
      541 ttcgagctgc cgcaggcctt cttcatgcga gtggcgatgg ggcttgcgct gaacgaggtc
      601 gaccgcgagg cgcgcgccat cgaattctat cggctgctgt ccagcttcga attcatgagc
      661 agcacgccca cgctgttcaa tgccggcacg ctgcgcccgc agctctcttc ctgctatctc
      721 accaccgtgc ccgatgatct cgacggcatt ttcggcgcca tccgcgacaa cgccctgttg
      781 tccaagttcg ccggtggcct cggcaacgac tggacgcggg tgcgcggact cggcgcgctc
      841 atcaagggca cgcacggcaa gagtcagggt gtggtgccct tcctcaaggt tgccaacgac
      901 accgcggtgg cggtcaatca gggcggcaag cgcaagggcg cggtctgcgc ttacctcgag
      961 acctggcaca tcgacatcga ggagttcctc gagctgcgca agaacacggg tgatgagcgc
     1021 cgccgcaccc acgacatgaa caccgcccac tggattccgg acctgttcat gcgccgggtc
     1081 tgcgaggagg ccgactggac gctgttttcg ccggacgaaa ccccggatct gcacgacctc
     1141 gtcggcgcgg ccttcgaggc gcgctatctc gcgtacgagc agctcgccgc ggccggcggt
     1201 ctgcgccatt acaaacgcat cccggcggtg cagctgtggc gcaagatgct ctccatgctg
     1261 ttcgagacgg gtcacccttg gctcaccttc aaagacgcct gcaacctgcg ctataccaat
     1321 gcgcatctgg ggcgggtgca cagctccaac ctgtgcaccg agatcaccct gcacaccagc
     1381 gatgaggaga tcgccgtgtg caacctaggc tcgatcaatc tcgccgctca tgtggacgcc
     1441 ggcgtcctcg atgaggacaa gctcgtgcgc accatcacca ccgccgtgcg catgctcgac
     1501 aacgtcatcg acatcaactg gtatagcgtg ccccaggcgc gccgctccaa cctgcgccat
     1561 cggccggtgg gcctgggcct catggggttt caggacgccc tgcaccggat gcaactcgcc
     1621 tacgcctcac cggcggcagt ggaattcgcc gatcgctcca tggagctgat cagctatcac
     1681 gccatcgcgg catcatccgc gctcgccgcc gaacgcgggc gctatccgag ctacgagggt
     1741 tcgctgtgga gtcagggcat tttgccgatc gactcgctgg cgctgctggc cgaggcgcgc
     1801 ggggagacgc tcgaggtcga ccgcagcgcc acgctcgact gggcgagcct gcgcgagcag
     1861 gtgcgccggc agggcatgcg caacggcaat gtcctcgcga tcgcgccgac cgcgaccatc
     1921 gccaacatct gcggggtgag cgcctcgatc gagccgacct tccagaacct gttcgtcaag
     1981 tccaatctct ccggcgagtt caccgtcgcc aacccttacc tcgtgcgcga gttgcaggcg
     2041 ctggggttgt gggatccggt gatgatcaat gatctcaagt actacgacgg cagcctgggg
     2101 ccgatcgcgc gggtgccggc agagctcaag gcgcgctacg cctgcgcctt cgagatcgat
     2161 ccggtatggc tggtggaggc ggcggcgcgg cggcagaagt ggctggacca ggcgcagtcg
     2221 ctgaatctct acgtcgccga ggcttcgggc gcccagctcg atgcgctcta caagctggcc
     2281 tggcggcggg gcctcaagac cacctattac ctgcgcaccc gcggcgccac ccatctggag
     2341 aagg
//
LOCUS       DAOQNN010000479         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_36542_length_2331_cov_14.894552, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000479 DAOQNN010000000
VERSION     DAOQNN010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36542_length_2331_cov_14.894552"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PKV97_16365"
     CDS             complement(<1..659)
                     /locus_tag="PKV97_16365"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB07508.1"
                     /translation="MAKLTELTVPKILHHEMPFTRLEIVVMSIIEGRLEVLLGRRAQA
                     PNQGRWALPGGVLRIDTDRDLDAAALRIGVERLGVELPYLRQLKAVGGRTRDPRAPWT
                     LSVVYRALVPIEAIEPNPGKRLEALKWDPAEEAGADVRLAFDHNELVQVAVEVTRGEI
                     DRLDLPFGFLPETFTLGELQACCEAILGRRLDKSSFRRRLDERNLLEAVPGEMRTGAF
                     RP"
     gene            731..1672
                     /locus_tag="PKV97_16370"
     CDS             731..1672
                     /locus_tag="PKV97_16370"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB07509.1"
                     /translation="MDCDIIGDVHGQDGKLVALLGKLGYRIKDGAYRHPQGRMALFLG
                     DLIDRGPGQIEVVNIVRRMIDAGSARSIMGNHEWNAIGFGMRDPDGPDFLRPRTESKI
                     REHKAFLEQVGLDSPLHEELVGWFATLPPVLDLGEIRLCHAWWNPDSIDTLGAAMDGD
                     GALDQEFLIASYRKRSTPWQAMECVTKGCEIRLPDGSSFRDHNDVTRKDIRVRWWDAS
                     VTGYRRAALVPDSERERIPDLPLPADLELGAVSDVPTFVGHYWLSGTPGVQNPTTAVL
                     DYGAAIKGPLVAYRWEGEKALSNDKLVWVQPNGGSGT"
     gene            1669..>2331
                     /locus_tag="PKV97_16375"
     CDS             1669..>2331
                     /locus_tag="PKV97_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07510.1"
                     /translation="MKVTVPDGAIGHVGMVAALKRVAEPDVGKLVIVCEPAGFVTSLV
                     GSVQPVFAWLAQSLGEPIDCQGRSSRTLYVADRCLIPLTGMSAAETSEIIRSQNQADF
                     DAALEHAGVALDAHEIGDDEFEKLLAKAAEQAFVGRSLEVVATPVALQEVGFRPVSGT
                     EGSLLFTGLHEGVELQIHADPEWISNWRLTGISHSPRHAQYTEKVLPNELPRGEIVRA
                     VLD"
BASE COUNT          419 a          750 c          756 g          406 t
ORIGIN      
        1 ggccgaaacg ccccggtacg catctctccc ggcaccgctt ccaggagatt tcgctcatca
       61 aggcgccgcc ggaagctcga cttgtccagg cgccggccga ggatggcctc gcagcaggct
      121 tgcagttccc ccagcgtaaa ggtctccggc aagaaaccga acggcagatc tagccggtcg
      181 atctcgccac gggtcacctc gaccgccacc tggaccaact cgttgtggtc gaatgccagc
      241 cgtacatcgg cgccagcctc ttcggcggga tcccacttca aagcctctag ccgcttgccc
      301 ggattcggct cgatggcctc aatcggcacc aacgcccggt agaccacgct caaggtccac
      361 ggcgcccgcg gatcgcgcgt ccgtccgccg accgccttga gctggcgcag gtaaggcagt
      421 tcgaccccca gccgctcgac gccgatgcgc agcgccgccg catccaagtc gcggtccgtg
      481 tcgatgcgca gcaccccgcc cggcagcgcc catcttccct gattcggcgc ttgggcgcgt
      541 cggccgagca gcacctcgag ccgcccctcg atgatcgaca tgacgacgat ctcgaggcgg
      601 gtaaacggca tctcgtgatg aaggattttc gggacggtca gttctgtaag tttcgccata
      661 gttgtgttgt gctacgaagt ggcgtacagt gtgttcgtag tgtattgcaa ctaagtaacg
      721 ggagcaatcc gtggactgcg acatcatcgg cgacgtacac gggcaggacg gcaaactggt
      781 cgccctgctc ggcaagctcg gctaccggat caaggatggc gcctaccggc acccgcaagg
      841 ccgcatggca ctgtttctcg gcgatctcat cgatcgtggc ccgggccaga tcgaggtggt
      901 aaatatcgtg cgccggatga tcgacgccgg cagcgcccgt tcgatcatgg gtaaccacga
      961 atggaatgcg atcggcttcg gcatgcgcga cccggatggc cccgactttc tgcgcccccg
     1021 caccgagagc aagatccgcg aacacaaggc gtttctcgag caggttggcc tggattctcc
     1081 gctgcacgag gaactggtgg gctggtttgc gaccttaccg ccggtcctcg acttgggtga
     1141 gatccgcctc tgtcacgcct ggtggaaccc cgacagtatc gacacgctgg gtgccgcaat
     1201 ggacggcgac ggggcgctgg atcaggagtt cctgatcgcc tcgtaccgca agcgcagtac
     1261 gccctggcag gccatggagt gcgtcaccaa gggttgcgag atccggctac ctgacggaag
     1321 cagctttcgc gatcacaacg atgtcacgcg caaggatatt cgcgtacgct ggtgggatgc
     1381 gtccgtgacg gggtatcggc gcgccgcact ggtgccggac agcgagcggg agcgcattcc
     1441 ggacctgccc ctgcctgccg acctcgaact gggcgccgta agcgatgtac cgacctttgt
     1501 cgggcactac tggctcagcg gcacgccggg ggtgcagaac cccaccacag ccgtgctcga
     1561 ttacggcgcg gcgatcaagg ggccgctggt ggcctaccgc tgggagggcg agaaggcgct
     1621 gagcaacgac aaactggtct gggtgcaacc gaatgggggc agcggcacat gaaggtcacc
     1681 gtcccggacg gtgccatcgg ccatgtcggc atggtggccg cactcaagcg ggtcgcggag
     1741 ccggacgtcg gcaaactggt gattgtgtgc gaaccggcgg gattcgtgac ctccctggtg
     1801 gggagtgttc agccggtgtt tgcctggctc gcccagtcgc tcggggaacc catcgactgc
     1861 cagggtcggt ccagccgaac cctttatgtg gcggaccgct gcctgatccc gctcaccgga
     1921 atgtcggcgg ccgagacgag cgagatcatt cggtcgcaga accaggccga cttcgatgcc
     1981 gcgctggaac atgccggggt tgccctcgac gcccatgaaa tcggcgacga tgagttcgag
     2041 aagctgctgg ccaaggcagc cgaacaggcc ttcgttggca ggtccctgga ggtggtagcc
     2101 acacccgtcg ccctgcagga ggtgggattc cgaccggttt ccggcaccga ggggtcactg
     2161 ctcttcaccg gcctgcatga gggtgtcgag ctgcagatcc atgccgatcc ggaatggatc
     2221 tcgaactggc gtctgaccgg aatctcccat tcgcctcgcc acgcgcaata caccgagaag
     2281 gtattgccca acgaactgcc gcgaggcgag atcgtccgcg ctgtgttgga c
//
LOCUS       DAOQNN010000480         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_36900_length_2318_cov_7.307556, whole genome shotgun sequence.
ACCESSION   DAOQNN010000480 DAOQNN010000000
VERSION     DAOQNN010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36900_length_2318_cov_7.307556"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PKV97_16380"
     CDS             complement(<1..474)
                     /locus_tag="PKV97_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNB07511.1"
                     /translation="MKNPRPLLLAVLLAHVAGAVCAADWQLVLSDRDRRIEIDRASVL
                     RSDAGTKISWGRVVLTSEEARRSGYAMIKALNRYDCRNRSFATVKRVYVDADDNIVRE
                     EPVAAEAPIAVARNTVDERMWREVCDPPSAVELQKMAAEAARIASAQPAAMAAPGG"
     regulatory      572..667
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            661..1554
                     /gene="thiD"
                     /locus_tag="PKV97_16385"
     CDS             661..1554
                     /gene="thiD"
                     /locus_tag="PKV97_16385"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNB07512.1"
                     /translation="MSSSASSAAPGSAPSASSGTASRIPNVVSIAGVDPSGGAGVFAD
                     LKTFSALGAYGCGVVAALTAQNTRTVTGVHVPPTDFLRLQIDTLFEDVAIHATKIGML
                     GSAEVTTTVAERLAHWQAARVVLDPVMVAKSGDSLLAKSAISALREALFPQAFLITPN
                     LPEAGVLLERRAPDTVKEMYRAAERLREFLPLSSERWVLLKGGHLPGNDLVDLLFDGD
                     RMVELPAQRIETKNTHGTGCTLSSAIAALLPQAEGQFRPVETAVRHARQWLLGAIARS
                     GELAVGSGHGPVHHFHALWRK"
     gene            1572..>2318
                     /locus_tag="PKV97_16390"
     CDS             1572..>2318
                     /locus_tag="PKV97_16390"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNB07513.1"
                     /translation="MNANEKFIAAKAHVDEAAIAPLPNSRKIHVEGSRPDIRVPMREI
                     TQADTPASFGAEQNPPIFVYDCSGPYSDPAAKIDIRSGLPALRQQWIEERGDTELLPD
                     LSSEFGRQRAADKTLDELRFPGLHRKPRRAKAGANVTQMHYARRGIITPEMEFVAIRE
                     NLNREQYIASLRASGGLKGQKMADMMLRQHPGQNFGASLPATITPEFVRDEIARGRAI
                     IPNNINHPESEPMIIGRNFLTKINANIGNSA"
BASE COUNT          379 a          887 c          730 g          322 t
ORIGIN      
        1 gcccccaggg gccgccatcg ccgccggctg cgcgctcgcg atccgggccg cctcggccgc
       61 catcttctgc agctcgaccg ccgagggcgg atcgcacacc tcgcgccaca tgcgctcgtc
      121 caccgtgttg cgcgccaccg cgatcggcgc ctcggccgcc accggctcct cgcgcacgat
      181 gttgtcgtcg gcatcgacat acacccgctt gaccgtggcg aagctgcggt tgcggcagtc
      241 gtagcggttg agcgccttga tcatggcata gccggagcgc cgcgcctcct ccgaggtcag
      301 caccacccgc ccccaggaga tcttggtgcc ggcgtccgag cggaggacgc tcgcgcgatc
      361 gatctcgatg cggcgatcgc ggtcgctcaa cacgagctgc cagtcggcgg cgcagaccgc
      421 gccggcgaca tgggcgagca gcacggcaag aaggagaggg cgcgggtttt tcatcggcgt
      481 cggcatggga tggcgttcca tcctgctaaa cgaccgcgag ggcgggatct tgagcggctt
      541 gccacgcgaa agtgcataca gtatcttccc acgctctcgt taggggtgcc cgccgcgcac
      601 gggctgagaa acacccttgg aacctgatcc ggctcgcacc ggcgtaggga aacgtcgttc
      661 atgagttcgt ccgcaagctc cgctgccccc ggcagcgcgc cgtccgcatc gtccgggacg
      721 gcttcccgca tcccgaacgt ggtgtccatc gccggcgtcg acccgtccgg cggcgccggc
      781 gtgttcgccg acctcaagac cttttccgcg ctcggcgcct acggctgcgg cgtggtcgcc
      841 gcacttaccg cgcagaacac ccgcaccgtc accggcgtgc atgtgccgcc caccgacttc
      901 ctgcgcctgc agatcgacac cctgttcgag gacgtcgcca tccacgccac caagatcggc
      961 atgctcggca gcgccgaggt caccaccacg gtggccgagc gcctggcgca ctggcaggcg
     1021 gccagggtgg tgctcgaccc cgtcatggtg gccaagagcg gcgacagcct gctggcgaag
     1081 agcgcgatct cggcgctgcg cgaggcgctc ttcccgcagg ccttcctgat cacccccaac
     1141 ctgcccgagg ccggcgtgct gctcgagcgg cgcgcacccg acacggtcaa ggagatgtac
     1201 cgcgccgccg agcgcctgcg cgagttcctg ccgctgtcct ccgagcgctg ggtgctgctg
     1261 aagggcggcc acctgcccgg caacgacctg gtcgacctgc tgttcgacgg cgaccgcatg
     1321 gtcgagctgc cggcgcaacg catcgaaacg aagaacaccc acggcaccgg gtgcacgctc
     1381 agctccgcga tcgccgcgct gctgccgcag gccgaaggcc agttccgccc ggtcgagacc
     1441 gcggtgcgcc acgcccgcca gtggttgctg ggcgcgatcg cgcgctccgg cgaactcgcg
     1501 gtcggcagcg gccacgggcc ggtgcatcac ttccacgcgc tgtggcgcaa gtaagccccc
     1561 caggagacag catgaacgcc aacgaaaaat tcatcgccgc caaggcccac gtcgacgagg
     1621 ccgcgatcgc cccgctgccc aactcgcgca agatccacgt cgagggctcg cgccccgaca
     1681 tccgcgtgcc gatgcgcgag atcacccagg ccgacacccc ggcctccttc ggcgccgagc
     1741 agaacccgcc gatcttcgtc tacgactgtt ccggccccta ctccgacccg gcggcgaaga
     1801 tcgacatccg ctccggcctg cccgcgctgc gccagcagtg gatcgaggag cgtggcgaca
     1861 ccgagttgct gcccgatctt tcgtccgaat tcggccgcca gcgcgccgcc gacaagaccc
     1921 tggacgagct gcgcttcccc ggcctgcacc gcaagccgcg ccgcgccaaa gccggtgcga
     1981 atgtcaccca gatgcactac gcccgccgcg gcatcatcac gcccgagatg gaattcgtcg
     2041 ccatccgcga gaacctgaac cgcgagcagt acatcgcctc gctgcgcgcc agcggcggcc
     2101 tcaagggcca gaagatggcc gacatgatgc tgcgccagca ccccgggcag aacttcggcg
     2161 ccagcctgcc ggcgacgatc acccccgagt tcgtgcgcga cgagatcgcc cgcgggcgcg
     2221 ccatcatccc caacaacatc aaccaccccg agagcgagcc gatgatcatc ggccgcaact
     2281 tcctcaccaa gatcaacgcc aacatcggca actcggcg
//
LOCUS       DAOQNN010000481         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_37400_length_2301_cov_8.418077, whole genome shotgun sequence.
ACCESSION   DAOQNN010000481 DAOQNN010000000
VERSION     DAOQNN010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37400_length_2301_cov_8.418077"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /gene="pcnB"
                     /locus_tag="PKV97_16395"
     CDS             complement(<1..1261)
                     /gene="pcnB"
                     /locus_tag="PKV97_16395"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNB07514.1"
                     /translation="MIRKLLRKVFKRPATPARHEPALIPVERHGIRREQLSPAARKVC
                     AVLQEKGFKAFVVGGAVRDLLIGHPPKDYDVATSATPEEVRAAFRRSRIIGRRFKIVH
                     VMSGPETIEVSTFRASQDAESADTDEHGRVLRDNVFGNQQEDATRRDFTVNALYYDPG
                     TETIVDYHHGVADIQQKTLRMIGDPRTRYREDPVRMLRAVRLAAKLGLTIDPAARQPI
                     REMGPLLENVPAARLFDEMLKLLFSGHSLKCLQQLRAEGLHHGLLPLLDVILEQPMGE
                     RFVTLALENTDERVREDKSVSPGFLFSTLLWHEVLKTWDNKKAAGDHSQPALFEAMDE
                     VLDAQAGKLAITRRIAGDIKDIWALQPRFDKRAGKAPYRVIEQPRYRAGWDFLRLRAQ
                     AGEIPMEIVDWWDDFAHAGPDQREALLR"
     gene            complement(1645..>2301)
                     /locus_tag="PKV97_16400"
     CDS             complement(1645..>2301)
                     /locus_tag="PKV97_16400"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNB07515.1"
                     /translation="LVLFDLDNTLLAGDSDFAWAQFLIGKGVLDREVQEAKNIAFYEQ
                     YKAGTLDIFEFLDFQLAPLARHPRAQLDAWHCEFMAQSIRPMITAKARTLVREHLDSG
                     ALVAVVTATNAFVTGPIVREFGIPHVVATIPAQQDGAFTGKPRGMPAFKAGKIERVDA
                     WLESLGLHLGSFEHSWFYSDSHNDLPLMQRVTDPVAVDPDDTLRAHAEAAGWPVISLR
                     "
BASE COUNT          340 a          707 c          879 g          375 t
ORIGIN      
        1 ggcgcagcag ggcctcgcgc tggtcggggc cggcgtgggc gaagtcgtcc caccagtcga
       61 cgatctccat cgggatttcg ccggcctgcg cgcgcaggcg caggaaatcc cagccggcgc
      121 ggtagcgcgg ctgctcgatg acgcggtagg gcgccttgcc ggcacgcttg tcgaagcgcg
      181 gctgcagcgc ccagatgtcc ttgatgtcgc cggcgatgcg gcgggtgatg gcgagcttgc
      241 cggcctgggc gtcgagcacc tcgtccatgg cctcgaagag tgcgggctgg ctatggtcgc
      301 cggcggcctt cttgttgtcc caggtcttca gcacctcgtg ccagagcagg gtggagaaca
      361 ggaagccggg cgaaacggac ttgtcctcgc gcacgcgctc gtcggtgttc tccagtgcca
      421 gggtgacgaa gcgctcgccc atcggctgct cgaggatcac gtccagcagt ggcagcaggc
      481 cgtggtgcag gccttcagcg cgcagctgct gcaggcactt cagcgagtga ccggagaaca
      541 gcagcttgag catctcgtcg aagagccgcg ccgccggcac gttctcgagc agcgggccca
      601 tctcgcggat cggctggcgc gctgcggggt cgatggtcag cccgagcttg gccgccaggc
      661 gcaccgcgcg cagcatgcgc accgggtcct cgcggtagcg cgtgcgcggg tcgccgatca
      721 tgcgcagggt cttctgctgg atgtcggcga cgccgtggtg gtagtcgacg atggtctcgg
      781 tgccgggatc gtagtacagc gcgttgaccg tgaagtcgcg ccgggtggcg tcttcctgct
      841 ggttgccgaa gacgttgtcg cgcagcacgc ggccgtgctc gtcggtgtcg gcgctctcgg
      901 cgtcctggct ggcgcggaag gtggagacct cgatggtctc cggcccgctc atcacgtgca
      961 cgatcttgaa gcgccggccg atgatgcgcg aacggcggaa ggcggcgcgc acctcctcgg
     1021 gtgtggcgct ggtggcgacg tcgtagtcct tgggcgggtg gccgatcagc aggtcgcgca
     1081 ccgcgccgcc gacgacgaag gccttgaagc ccttctcctg cagcaccgcg cataccttgc
     1141 gcgcggccgg cgacagctgc tcgcggcgga tgccgtggcg ttcgacggga atgagggcgg
     1201 gttcgtggcg tgcgggggtg gccgggcgct tgaaaacctt gcgcagcagc ttgcggatca
     1261 tcgggaaagg agtcgtgctt gcataagcgc gcaatgataa ccgatcgaaa cgtccgtacg
     1321 ctgcgcgaag gcggagggga gcggtgggga ttcggcaaaa tggcactccc gactcgcgtc
     1381 ccgcccgcgg aaaagctgcg cgcttccggg gcggctgcga tcgcgacttg cgtcgctcct
     1441 acagggaaac cggtgcgtct tcggggcggt ggcggtggcg acttgcgtcg ctcccacgca
     1501 aaccgtcacg cacccggagc cgcgatgtgg gagcgacgca agtcgcgatc ggcggtgcgg
     1561 gaggcgatga gcgtcggcgg gtgcggggtg gtgtaggagc gacgcaagtc gcgaatcttc
     1621 agcgcagcga gatcaacgac cagttcagcg cagcgagatc accggccagc cggcggcctc
     1681 ggcgtgggcg cgcagggtgt cgtcggggtc gaccgcgacc gggtcggtga cgcgctgcat
     1741 cagcggcagg tcgttgtgcg agtcgctgta gaaccagctg tgctcgaagc tgcccagatg
     1801 caggccgagc gactccagcc aggcatcgac gcgctcgatc ttgcccgcct tgaacgccgg
     1861 catgccgcgc ggcttgccgg tgaaggcacc gtcctgctgt gcggggatgg tggccaccac
     1921 gtgcgggatg ccgaactcgc gcacgatcgg accggtgacg aaggcgttgg tggcggtgac
     1981 caccgcgacc agggcgccgc tgtcgaggtg ctcgcgcacc agcgtgcgcg ccttggcggt
     2041 gatcatcgga cggatcgact gcgccatgaa ctcgcagtgc caggcgtcga gttgcgcgcg
     2101 cgggtggcgg gcgagggggg cgagctggaa gtcgaggaat tcgaagatgt cgagcgtgcc
     2161 ggccttgtac tgttcgtaga acgcgatgtt cttcgcttcc tgcacctcgc ggtcgagcac
     2221 gcccttgccg atgaggaact gcgcccaggc gaagtcggag tcgccggcga gcagggtgtt
     2281 gtcgaggtcg aagaggacga g
//
LOCUS       DAOQNN010000482         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_37479_length_2298_cov_9.496210, whole genome shotgun sequence.
ACCESSION   DAOQNN010000482 DAOQNN010000000
VERSION     DAOQNN010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37479_length_2298_cov_9.496210"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PKV97_16405"
     CDS             complement(<1..503)
                     /locus_tag="PKV97_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07516.1"
                     /translation="MSARARDLVLGTLLPLLALDALLVFDNAWPTLWPRPTPAVSIEL
                     AFAVAALVLFAAWRKHAAHGLVRVLAGLATLWIVLRYVQVTVPALFGRPLNLYWDLPH
                     LGAVLDMGGDGPGAKVLLALALGILVVLVLHRVVSACVRALARSAAAPGARALLGGVA
                     GAAILLWA"
     gene            638..1465
                     /locus_tag="PKV97_16410"
     CDS             638..1465
                     /locus_tag="PKV97_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNB07517.1"
                     /translation="MNAKPALPWWQDLTPSRIAGLARADAVALLPLAAIEQHGEHLPL
                     STDLDIALGLLEAAVPKLRAGLPLCVLPPVAVGCSLEHTGFAGTLALDAKTALASVVE
                     IGACVARAGLRRLVLFNTHGGNKALVDLAALELRAAQRMLVVRAHSFRFPPPPAALPA
                     DELRHGLHGGALETALMMHLAPHKVRRDAVDRFESLGARMAAAGRLLGPEGEAGFAWM
                     AQDLHPSGACGDPRLADAALGARLVEHFASRLARIVEEAHDFDLEALVTCAPAPEQR"
     gene            complement(1498..>2298)
                     /locus_tag="PKV97_16415"
     CDS             complement(1498..>2298)
                     /locus_tag="PKV97_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNB07518.1"
                     /translation="RVPAALVCEVAPDRAAAVAATVASGDLLEVDAAEAMRLCESGPG
                     RLARISEARVPLAEAENSRFVLFREADGVHEHVAIVIGERANWPEDVPVRLHSSCLTG
                     DLFGSLRCDCGEQLRRGVAAIDALGGGVLLYLSQEGRGIGLANKLRAYGLQDEGLDTI
                     DADQVIGFSKDERDFRIAHEMLDELGIDRILLLTNNPSKVAALQKAGIHVVGRRAIYG
                     EVTAQNQRYLRTKASRHGHWLNDLLNEQGEPGDDEPACLPAADAAHRA"
BASE COUNT          314 a          821 c          863 g          300 t
ORIGIN      
        1 gcccacagca ggatcgccgc gcccgcgaca ccgccgagca gcgcacgcgc gcccggtgcc
       61 gccgcgctgc gtgcgagggc acgcacgcag gccgacacca cgcgatgcag taccagcacg
      121 acgaggatac cgagcgcgag cgcgagcagg accttcgcgc ccggaccgtc gccgcccatg
      181 tcgagcacgg cgccgaggtg gggcaggtcc cagtagaggt tgagcgggcg gccgaacagc
      241 gcggggacgg tcacctgcac gtagcgcagc acgatccaca gcgtggccag ccccgcgagc
      301 acgcgcacga ggccgtgcgc tgcgtgtttc cgccaggcgg cgaagagcac cagtgcggcg
      361 acggcgaagg cgagctcgat cgagacggcc ggcgtcggcc gcggccacag cgtcggccag
      421 gcgttgtcga acaccagcaa ggcgtcgagc gcgagcagcg gcaggagcgt gccgagcaca
      481 agatcacgcg cccgtgcgct catgcgaccc ctccgccgcg tgcggcaaag cgcgggcgcg
      541 cgggtcggca ccagggcgcg ggacacggcg acgtcgggcc gtggccgagt gcggctatga
      601 tccgggtcag ccgatgcccc ccgggagacc gaccgaaatg aacgccaagc ccgcgctgcc
      661 ctggtggcag gacctgaccc cgagccgcat tgccgggctc gcccgcgccg atgcggtcgc
      721 gttgctgccg ctcgccgcga tcgagcagca cggagagcac ctgccgctgt cgaccgacct
      781 cgacatcgcc ctcggcctgc tcgaggcggc cgtgccgaag ctgcgcgcgg gcctgccgct
      841 gtgcgtgctg ccgccggtcg cggtggggtg cagcctggaa cacacgggct ttgccggtac
      901 gctcgcgctc gacgcgaaga ccgcgctggc gagcgtggtg gagatcggtg cctgcgtcgc
      961 gcgcgccggc ttgcgccgcc ttgtgctgtt caacacccat ggcggcaaca aggcgctggt
     1021 ggacctggcc gcgctcgagc tgcgcgccgc gcagcgcatg ctggtggtgc gcgcgcattc
     1081 cttccgcttc ccgccgccac ccgccgcgct gccggcggac gaactgcgcc acggcctgca
     1141 tggtggcgcg ctggagaccg cgctgatgat gcatctggcg ccgcacaagg tgaggcgcga
     1201 tgcggtcgat cgattcgagt cgctgggggc gcgcatggcg gcggcggggc gactgctcgg
     1261 ccccgagggc gaggccggct tcgcctggat ggcgcaggac cttcatcctt cgggggcctg
     1321 cggcgatcct cgcctggcgg acgccgcgct cggggcgcgc ctggtggaac acttcgcgag
     1381 ccgtctcgcg cgcatcgtcg aagaggcaca cgacttcgac ctcgaagcgc tggtgacgtg
     1441 cgcaccggcg ccggagcagc gctgagcggg ccgcatggcc ggcctggagg ccgcgcctca
     1501 ggcgcggtgc gcggcgtccg cggcgggcag gcatgcgggc tcgtcgtcgc ccggctcgcc
     1561 ctgctcgttc agcagatcgt tgagccagtg gccgtgacgg ctggccttgg tcctgaggta
     1621 gcgctggttc tgcgcggtga cctcgccgta gatcgcgcgc cggccgacga cgtggatgcc
     1681 ggctttctgc agcgcggcga ccttgctcgg gttgttggtg agcagcagga tgcggtcgat
     1741 gccgagctcg tcgagcatct cgtgggcgat gcggaagtcg cgctcgtcct tggagaagcc
     1801 gatcacctgg tcggcgtcga tcgtgtccag tccctcgtcc tgcaggccat aggcgcgcag
     1861 cttgttggcc aggccgatgc cgcgaccctc ctgcgagagg tagagcagca cgccgccgcc
     1921 gagcgcgtcg atcgctgcca cgccgcggcg cagctgctcg ccgcagtcgc aacgcaggct
     1981 gccgaagagg tcaccggtga ggcaggacga gtgcaggcgc accgggacgt cctcgggcca
     2041 gttcgcgcgc tctccgatga cgatggcgac gtgctcgtgc acgccgtcgg cttcacggaa
     2101 gagcacgaag cggctgttct cggcctcggc cagcggcacg cgcgcctcgc tgatgcgggc
     2161 gagccggccg gggccgctct cgcacaggcg catggcctcg gcggcatcga cctcgagcag
     2221 atcgccgctc gccaccgtgg ccgcgaccgc agcggcgcga tcgggcgcga cttcgcacac
     2281 cagcgccgcc ggcacccg
//
LOCUS       DAOQNN010000483         2297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_37510_length_2297_cov_12.519625, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000483 DAOQNN010000000
VERSION     DAOQNN010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37510_length_2297_cov_12.519625"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..>2297
                     /locus_tag="PKV97_16420"
     CDS             196..>2297
                     /locus_tag="PKV97_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNB07519.1"
                     /translation="MKFSKAEVDAIIEASPRTFVPFNKLVLSEDHQARSATAPVMSLP
                     ELAASIKDSGVLQNLVVVQAARGRYEVCAGGRRLAALTLLVQQGDIADNYPVPVLVVP
                     ADKALIASLAENCFHVPMHPADEFAAFARLLAQGKSVEDVAAAFGVTPLIVKRRMKLA
                     TVSPRLMALYREGGIGLDCLMVLASVDDHQRQEQAWAGLPSWNQRPEHLRQLLTRGEI
                     ESDADPLARFVTVKAYEKAGGPLRRDLFSDDDRKAYLLDPALLERLAADKLGKRAKQL
                     LSEGWKWVDIRPRFAYDEYVKHGELRKPRRAPTPEEAEALKALDTRIAALHEQMDALV
                     DRDGDGDAGEGVSGASEANDAADEDDASRDRAFLALESEADGLEEERKARLEALTEWP
                     VAWMALAGCVLHVGADGRAAIRAGLIRPEDRADLAQAITKAAEQKSSGGGGSEGDADP
                     ARGSGLPALQSLPSAAGATARPVHSEKLMRRLTAHRVAAIQAELLDRPEVALAALTTH
                     LTLKLLADPLHGAYRLPNLLAISATGNQHELRGAAEDIEASPAAQRIEADRIAWIEHL
                     PKEPDAVFDWMLRQPPQTVQRLLTFLVASTVNGITGTDAGRPVNDGLARALGLDMTRW
                     WSATGEAYLQHVSKARVVEVVHAVAGAKVAGPLAALKKDAAVAQAEQLLAGRGWLPDC
                     LRVQPHADAPATGGEEVDA"
BASE COUNT          363 a          812 c          818 g          304 t
ORIGIN      
        1 ggcaagcgcg aggtggtgga gcgcttggca gccctgaagt cgggcgagca gtcgtaggcg
       61 agccgacgcg cggtccggcg tcctgaccgg accaccggcg agcgctcctc gccacccttg
      121 aagtgcacgg gctcaatggg atcctgactg catggtgggc cgccggcctg ccatgcaacc
      181 gatcaggagt ccctcatgaa attctccaaa gccgaggtcg acgccatcat cgaggcgtcc
      241 cctcgcacct tcgttccctt caacaagctg gtgctgtccg aggaccacca ggcccgcagt
      301 gccacggcgc ccgtgatgtc gctgccggaa ctggccgcgt cgatcaagga cagcggcgtg
      361 ctgcagaacc tcgtcgtggt ccaggcggcg cgcgggcgct acgaggtctg cgccggcggg
      421 cggcggctcg ccgcgctcac gctgctcgtg cagcagggcg acatcgccga caactacccc
      481 gtgccggtgc tcgtcgtgcc ggccgacaag gcgctcatcg cgagccttgc cgagaactgc
      541 ttccacgtgc cgatgcaccc ggccgacgag ttcgccgcct tcgccagact gctcgcgcag
      601 ggcaagtccg tcgaggacgt ggccgcagcc ttcggcgtga cgccgctcat cgtcaagcgg
      661 cgcatgaagc tcgccaccgt ctcgccccgg ctgatggccc tgtaccgcga aggcggcatc
      721 ggcctggact gcctcatggt gctggcctcg gtcgacgatc accagcgcca ggagcaggcc
      781 tgggccggtc tgccgagctg gaaccagcgc ccggagcatc tgcggcagtt gctgacccgg
      841 ggcgagatcg aatcggacgc cgacccgctg gcccggttcg tcaccgtcaa ggcctacgag
      901 aaggccggcg gaccactgcg ccgcgacctg ttcagcgacg acgaccgcaa ggcctacctg
      961 ctggacccgg ccctgctgga gcgcctcgcc gccgacaagc tgggcaagcg cgcgaagcaa
     1021 ctgctgtctg aaggctggaa gtgggtcgac atccgtccgc gcttcgccta cgacgagtac
     1081 gtcaagcacg gcgagctgcg caagccccgc cgcgcaccga cgcccgagga agccgaggcg
     1141 ctgaaggcgc tggacacgcg catcgctgcc ctgcacgagc agatggacgc gctggtggac
     1201 cgcgacggcg atggcgatgc cggcgagggc gtcagcggcg ccagcgaggc gaacgacgcc
     1261 gccgacgagg acgacgcgtc gcgcgaccgt gccttcctcg ccctggagtc cgaggccgat
     1321 gggctggagg aagagcgcaa ggcgagactg gaagcgctga ccgaatggcc cgtggcctgg
     1381 atggcgctcg ccgggtgcgt gctgcatgtg ggcgcggacg gccgtgccgc gatcagggca
     1441 gggctcatcc gtcccgagga tcgggccgat ctggcgcagg ccatcaccaa ggctgccgaa
     1501 cagaagtcct ccggtggagg cggctcggaa ggcgacgcgg atcctgcgcg cggctcgggg
     1561 ctcccggccc tgcagtcgct tccatcggca gccggcgcca cggcacggcc cgtgcactcc
     1621 gagaagctca tgcgccgcct cacggcccac cgcgtcgccg cgatccaggc cgagctgctg
     1681 gaccgacccg aggttgcgct ggcggcactg acgacccacc tgacactgaa gctgctggcc
     1741 gacccgctcc atggcgccta ccgactgccc aacctgctgg cgatcagcgc gaccggcaac
     1801 cagcacgagc tgcggggcgc agccgaggac atcgaggcga gcccggcggc ccagcgcatc
     1861 gaagccgacc gcatcgcctg gatcgagcac ctgcccaagg aacccgacgc ggtgttcgac
     1921 tggatgctgc ggcagccgcc tcagacggtg cagcggctgc tgaccttcct ggtcgcgtcc
     1981 accgtgaacg gcatcaccgg caccgacgcc ggccgaccgg tcaatgacgg tctggcccgc
     2041 gccctcggcc tggacatgac gcgatggtgg tcggcgacgg gggaggccta ccttcagcac
     2101 gtgtccaagg cgagggtggt cgaggtggtg cacgcggtgg ccggcgccaa ggttgctggt
     2161 ccgctcgcgg cgctgaagaa ggatgccgcg gtggcgcagg ccgaacaact gctcgccggg
     2221 cgtggctggt tgcccgactg cctgcgcgtg cagccgcacg ccgatgcccc ggcgacgggt
     2281 ggcgaggagg tcgacgc
//
LOCUS       DAOQNN010000484         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_37647_length_2292_cov_9.286097, whole genome shotgun sequence.
ACCESSION   DAOQNN010000484 DAOQNN010000000
VERSION     DAOQNN010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37647_length_2292_cov_9.286097"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1297)
                     /locus_tag="PKV97_16425"
     CDS             complement(101..1297)
                     /locus_tag="PKV97_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNB07520.1"
                     /translation="MNPTRFAAGHAAHADWRVALARALDALLLDLRAAGGHDHAGRDG
                     LPEYTLGFCYLSDRFAADADEIVAELQRRLPGVHWVGTVGVGVAATGAEHFDEPALAL
                     MLAPLPRRAFRVFSGVQPLRADAEDFHPHTALVHADGRTPDLQELLPELAERTASGYL
                     FGGLTASRGRSVQIADGVFDGGGLSGVAFSAEVGVVSRVTQGCQPIGPRRLVSRARDN
                     FVITLDGRGALDCVMEDLGLPPDMALEPVAEALSNTLVGLHPGADAELVAPAAFGADM
                     LVRNIIGLDPRAGVVAIGDEVEQGAWLIFCRRDPAAALADLRRVAREIRDELVDSDRV
                     ALGAVYVSCSGRGGPHFGRPAAELELIRDVLGDVALAGFFAGGEIAHSRLYGYTGVLT
                     VFAAPR"
     gene            complement(1294..2196)
                     /locus_tag="PKV97_16430"
     CDS             complement(1294..2196)
                     /locus_tag="PKV97_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB07521.1"
                     /translation="MPARMHYTVLEPSSTERVAIRGLEYNVRRWGPADAPKVFLLHGW
                     MDTSATFQFVVDALRHDWQLIAPDWRGYGETTWLHRPYWFPDYYADLEALLAHYAPAG
                     PVRLVGHSMGANIAGIYAGLRPQRVARLAMLDFIGLKAPADVDAPQQLGRWLDAQLAP
                     PPARIYADRAALAERLRAVNPRLTPARAEFLCRHLGRVRADGQVEIAGDPWHKIPSPA
                     VYRIEDAMACWRRVRAPVLMLVSAEGYVHDRFGDEPEELQRRIDCFADARVVTVADCG
                     HNLQHDQPEVVAAELEAFLDREIP"
BASE COUNT          348 a          818 c          847 g          279 t
ORIGIN      
        1 ccttcgcgca gcgagcgcag ccacgacgac cgccgctcgc gcggctgacc cggcgggccc
       61 gcccagggcc cgagcctcgt gaagatcccg acgggcggcg tcagcgcggc gcggcgaaca
      121 cggtgagcac cccggtgtag ccgtagaggc ggctgtgggc gatctcgccg ccggcgaaga
      181 agcccgccag cgccacgtcg ccgagcacgt cgcggatcag ctcgagctcg gccgccggac
      241 ggccgaagtg cgggccaccg cggcccgagc agctcacata caccgcgccg agcgcgacac
      301 ggtcgctgtc caccagctcg tcgcgaatct cgcgcgccac gcggcgcagg tcggcgagcg
      361 ccgccgcagg gtcgcgccgg cagaagatca gccaggcgcc ctgctccacc tcgtcgccga
      421 tcgccaccac gccggcacgt ggatcgaggc cgatgatgtt gcgcaccagc atgtcggcac
      481 cgaaggcggc gggggcgacg agctccgcgt ccgcccccgg atgcagcccg accagggtgt
      541 tcgacaaggc ttcggcgacc ggctccagcg ccatgtcggg cggcaggccg agatcctcca
      601 tcacgcagtc gagcgcaccg cgaccgtcca gtgtgatgac gaagttgtcc cgcgcgcggc
      661 tcaccagccg ccgcggcccg atcggctggc agccctgggt gacgcgcgag acgacgccga
      721 cctcggcgga gaaggccacc cctgacaggc cgccgccgtc gaacacaccg tcggcgatct
      781 gcacgctgcg cccacgcgag gcggtcaacc cgccgaacag gtaaccgctc gcggtgcgct
      841 cggcgagttc gggcagcagc tcctgcaggt ccggggtgcg gccgtcggcg tgcaccagcg
      901 cggtgtgcgg gtggaagtcc tcggcatccg cacgcagcgg ctgcaccccc gagaacacgc
      961 ggaaggcgcg tcgcggcagg ggtgcgagca tcagcgcgag tgccggctcg tcgaagtgct
     1021 cggcaccggt cgcggccacc ccgacgccca cggtgccgac ccaatgcacg ccgggcaggc
     1081 ggcgctgcaa ctcggcgacg atctcgtcgg cgtcggcggc gaagcggtcg ctcaggtagc
     1141 agaagccgag ggtatattcg ggcagcccgt cccgccccgc atggtcatgg cctcccgcgg
     1201 cgcgcaggtc gagcagcagg gcgtcgagcg cgcgtgcgag cgccacccgc cagtcggcgt
     1261 gggcggcatg tccggcagcg aagcgggtcg ggttcatggg atctcccggt cgaggaacgc
     1321 ctccagctcg gccgccacca cctcgggctg gtcgtgctgc agattgtggc cgcaatcggc
     1381 cacggtgacg acgcgggcat cggcgaagca gtcgatgcgg cgctggagtt cctccggctc
     1441 gtcgccgaag cggtcatgca catagccctc ggcggagacc agcatcagca ccggggcacg
     1501 cacacgccgc cagcacgcca tcgcgtcctc gatgcggtag accgcgggcg aggggatttt
     1561 gtgccaggga tcgccggcga tctcgacctg tccgtcggca cgcacgcgcc cgaggtggcg
     1621 gcagaggaac tccgcacgcg ccggggtgag gcgcggattg accgcgcgca ggcgttccgc
     1681 gagcgcggcc cggtcggcgt agatgcgggc aggcggcggt gcgagctggg cgtcgagcca
     1741 gcgcccgagc tgctgcggcg catcgacgtc ggccggcgcc ttgagcccga tgaagtcgag
     1801 catcgccagc cgcgccacgc gctgcgggcg cagaccggcg tagatgccgg cgatgttggc
     1861 gcccatgctg tggccgacca ggcgcaccgg gccagcgggc gcgtagtggg cgagcagggc
     1921 ctcgaggtcg gcgtagtagt cgggaaacca gtaggggcgg tgcagccagg tggtctcgcc
     1981 gtagccgcgc cagtcggggg cgatcagctg ccagtcgtga cgcaacgcat cgacgacgaa
     2041 ctggaaggtg gcggacgtat ccatccagcc atgcagcagg aagaccttcg gcgcgtcggc
     2101 cgggccccag cggcgcacgt tgtattcgag gccgcggatg gcgacgcgtt cggtggagga
     2161 gggctcgagg acggtgtagt gcatgcgggc gggcatcttt tccgggacaa tcacgcttcc
     2221 attctagccg tcgattccat cccgccaccg tccacctggc atcctgccgg acgccacccg
     2281 cccgcccgca tg
//
LOCUS       DAOQNN010000485         2291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_37676_length_2291_cov_12.258945, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000485 DAOQNN010000000
VERSION     DAOQNN010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37676_length_2291_cov_12.258945"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="PKV97_16435"
     CDS             <1..96
                     /locus_tag="PKV97_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HNB07522.1"
                     /translation="LRLQPGYDHSYYFIASFIDEHLRHHAAALRG"
     gene            complement(199..>2291)
                     /locus_tag="PKV97_16440"
     CDS             complement(199..>2291)
                     /locus_tag="PKV97_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNB07523.1"
                     /translation="ATGIEEHDNYAVDFINAVAWIKENLPYAKTSGGVSNVSFSFRGN
                     DPVREAIHTVFLYHAIKAGLSMGIVNAGQLGVYDELAPELRDKVEDVVMNRKPGAGEA
                     LVEFAQTVKGQAKESAQDLAWREWAVEERLSHALVKGITEFVVADTEEVRAKLEAAGK
                     PPLAVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPYIEAEKLRTGA
                     SSKGRIVVATVKGDVHDIGKNIVGVVLGCNGYEVIDLGVMVPAAKILEAAKEHGAQAI
                     GLSGLITPSLEEMAHVAAEMKRQGFSVPLLIGGATTSRNHTAIKIAPHYDQPVVYVPD
                     ASRAVGVVTALLSEGRSEAFKAEVAADYEKIRALHANKKGMQLVSLEAARANAFRVAS
                     EYQPPRPNMLGVQAIDVELGDLVDYIDWGPFFQTWDLAGRFPAILDDEVVGETARNVF
                     ADGKAMLERIVAEEWLQAKAVFGLFPANAAGDDIEIYTGEDRKHLAMVWHGLRQQHER
                     PAGKPHWCLADFVAPKESGVPDWIGAFAVTAGLGIEARVAEFEAAHDDYHAIMLKSLA
                     DRLAEACAEWLHERVRRECWGYAADETLGNDALIREEYRGIRPAPGYPACPDHTVKGP
                     LFELLGARARIGMDLTESYAMTPAAAVSGFYFAHPESHYFAVSKIGRDQLEDWARRTG
                     MEVDEAARWLAPLL"
BASE COUNT          321 a          820 c          772 g          378 t
ORIGIN      
        1 ctgcgcctgc agccgggtta cgaccacagc tactacttca tcgccagctt catcgacgag
       61 cacctgcgcc accacgccgc cgcgctgcgc ggctgaagcg ccgtgttcgc gccttgcggc
      121 gcgcctgcat gaagattctc gcgcgtcttc cgtaggcgcg ccgcaaggcg cgaagcgggc
      181 cgcgggacga gcgccctctc acagcagcgg cgccagccag cgtgcggcct cgtcgacctc
      241 catgccggtg cggcgcgccc agtcctcgag ctggtcgcgg ccgatcttgg agacggcgaa
      301 gtagtggctc tcggggtggg cgaagtagaa gccgctcacc gccgccgccg gcgtcatcgc
      361 gtagctctcg gtgaggtcca tgccgatgcg cgcgcgcgcg ccgagcagct cgaacagcgg
      421 gcccttgacg gtgtggtccg ggcaggccgg gtagcccggc gcgggacgga tgccgcggta
      481 ctcctcgcgg atcagcgcat cgttgccgag cgtctcgtcg gcggcatagc cccagcactc
      541 gcggcgcacg cgctcgtgca gccactcggc gcaggcctcg gcgaggcgat cggccaggct
      601 cttcagcatg atggcgtggt agtcatcgtg cgcggcctcg aactctgcga cccgggcctc
      661 gatgccgagg ccggcggtga ccgcgaaggc gccgatccag tccggcacgc cggattcctt
      721 gggcgcgacg aagtccgcca ggcaccagtg cggcttgccc gcggggcgct cgtgctgctg
      781 gcgcaggccg tgccagacca tggcgaggtg cttgcggtcc tcgccggtgt agatctcgat
      841 gtcgtccccc gccgcgttgg ccgggaacag cccgaacacc gctttggcct gcagccactc
      901 ctcggcgacg atcctctcca gcatcgcctt gccgtcggca aagacgttgc gcgcggtctc
      961 gcccaccact tcgtcgtcga ggatggcggg gaagcggccg gcgaggtccc aggtctggaa
     1021 gaaggggccc cagtcgatgt aatcgaccaa gtcgccgagc tcgacgtcga tcgcctgcac
     1081 gcccagcatg ttcgggcgcg gcggctggta ttcggaggcg acgcggaagg cgttggcgcg
     1141 cgcggcctcg aggctcacca actgcatgcc cttcttgttg gcgtgcagcg cgcggatctt
     1201 ctcgtagtcg gcggcgacct cggccttgaa ggcctcgctc cggccttcgg acagcagcgc
     1261 ggtgaccacg cccaccgcac gcgaggcgtc gggcacgtac accaccggct gatcgtagtg
     1321 cggcgcgatc ttgatcgcgg tgtggttgcg gctggtggtg gcgccgccga tcaacagcgg
     1381 cacggaaaag ccctgacgct tcatctcggc ggcgacgtgg gccatctcct cgagcgaggg
     1441 cgtgatcagg ccggacaggc cgatcgcctg cgcgccgtgc tccttcgccg cctcgaggat
     1501 cttcgctgcc ggcaccatca cgccgaggtc gatcacctcg tagccattgc agccgagcac
     1561 cacgccgacg atgttcttgc cgatgtcgtg cacgtcgccc ttgaccgtgg cgacgacgat
     1621 acggcccttg ctgctggcgc cggtgcgcag cttctcggcc tcgatgtagg ggatcaggtg
     1681 cgccaccgcc tgcttcatga cgcgcgccga cttcaccacc tggggcagga acatcttgcc
     1741 cgcgccgaag aggtcgccga ccacgttcat gccggccatc agcgggccct cgatcaccgc
     1801 cagcggcggc ttgcccgcgg cctccagctt cgccctcacc tcctcggtat cggcgacgac
     1861 gaactcggtg atgcccttga ccagcgcgtg gctcaagcgc tcctcgaccg cccactcgcg
     1921 ccaggcgagg tcctgcgcgg actccttggc ctggcccttc accgtctgcg cgaactcgac
     1981 cagcgcctcg cccgccccgg gcttgcggtt catcaccacg tcctcgacct tgtcgcgcag
     2041 ctcgggcgcg agctcgtcat agacgccgag ctggccggca ttgacgatgc ccatcgacaa
     2101 gccggccttg atcgcgtggt agaggaacac ggtgtggatc gcctcgcgca ccgggtcgtt
     2161 gccgcggaag ctgaaggaca cgttcgacac cccgcccgag gtcttggcgt acggcaggtt
     2221 ctccttgatc caggccaccg cgttgatgaa gtcgaccgcg tagttgtcgt gctcctcgat
     2281 gccggtggcg a
//
LOCUS       DAOQNN010000486         2280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_37994_length_2280_cov_11.100674, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000486 DAOQNN010000000
VERSION     DAOQNN010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37994_length_2280_cov_11.100674"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /gene="pth"
                     /locus_tag="PKV97_16445"
     CDS             complement(<1..461)
                     /gene="pth"
                     /locus_tag="PKV97_16445"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNB07524.1"
                     /translation="MSNAAARPPRLIVGLGNPGAEYSETRHNAGFWFCERLADQLGAR
                     YSFESRFHGLVANAREAGMWLLMPQTFMNRSGQAIGALARFYRIEPAEILVVHDELDI
                     PPGQLRLKFGGGLGGHNGLKDTSAHLGTNDYWRLRIGIGHPGDRNEVVNFVL"
     gene            complement(495..1097)
                     /locus_tag="PKV97_16450"
     CDS             complement(495..1097)
                     /locus_tag="PKV97_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNB07525.1"
                     /translation="MQIEFKASKRDAQGTGASRRLRRAGRIPGILYGGAGETQPVSMD
                     HNELYHLLRKEAFHASVLSIEVDGAKQNAVLRDVQWHPFKQQVLHLDFQRIAAGEKMH
                     LKVPLHFVGADVAPAVKLGGCMISHTINELDVQCLPTDLPEFIEVDLSALEAGQSVHV
                     SQIKLPAGVELVQHGEGDPVVATALQVKGAAEAEGEGEQA"
     gene            complement(1185..2135)
                     /locus_tag="PKV97_16455"
     CDS             complement(1185..2135)
                     /locus_tag="PKV97_16455"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNB07526.1"
                     /translation="MPHGSLMVFTGNANPKLGADVTRRLGISLGSATVGRFSDGEVNV
                     ELLENVRGKDVFVLQPTCSPTNDNLMELLVLVDALKRASAGRITAAIPYFGYARQDRR
                     PRSARVPITAKVVANMLQAVGVQRLLTMDLHADQIQGFFDIAVDNVYAAPVLLADLDK
                     QKYDDLLVVSPDVGGVVRARAFAKRMECDLAIIDKRRPKANVSEVMNIIGEVEGRTCV
                     IMDDIVDTAGTLCKAAAALKKHGAKRVLSYCTHAVLSGAAVSRINESELDELVVTDTI
                     PLRDDAKACPRIRQISVASLLADTILRISNEESVSSLFME"
BASE COUNT          360 a          705 c          828 g          387 t
ORIGIN      
        1 agcacgaagt tcaccacctc gttgcggtcg cccggatggc cgatgccgat gcgcaggcgc
       61 cagtagtcgt tggtgccgag atgggcggag gtgtccttga ggccgttgtg gccgccgagg
      121 ccgccgccga acttgaggcg cagctggccg ggggggatgt cgagctcgtc gtgcacgacg
      181 aggatctcgg ctggttcgat gcggtagaag cgcgcgagcg caccgatcgc ctggccggag
      241 cggttcatga aggtctgcgg catcagcagc cacatgccgg cttcacgcgc gttggccacc
      301 aggccatgga aacgcgactc gaacgagtag cgcgcgccga gctggtcggc gagccgctcg
      361 caaaaccaaa acccggcgtt gtgccgggtt tcggagtatt cggcgcccgg attgccgagg
      421 ccgacgatca gacggggcgg acgcgcggcg gcgttgctca tcggtcgtcg gtctcctgca
      481 tccgggccgg gcgatcaggc ctgctcgcct tcgccttcgg cttcggcggc acccttcacc
      541 tgcagcgcgg tcgcgaccac cgggtcgcct tcgccgtgct gcaccagctc gacgccggcc
      601 ggcagcttga tctgcgagac gtgcaccgac tggccggctt ccagcgccga caggtcgacc
      661 tcgatgaact cgggcaggtc cgtgggcagg cactgcacgt cgagttcgtt gatggtgtgc
      721 gagatcatgc agccgccgag cttgacggcg ggggcgacgt cggcgccgac gaagtgcagc
      781 ggcaccttca ggtgcatctt ctcgcccgcg gcgatgcgct ggaagtcgag gtgcagcacc
      841 tgttgcttga acgggtgcca ttgcacgtcg cgcagcacgg cgttctgctt ggcgccatcg
      901 acctcgatcg agagcacgga agcgtggaag gcttccttgc gcagcaggtg gtagagctcg
      961 ttgtggtcca tcgacaccgg ctgggtttcg cccgcaccgc cgtagaggat gcccgggatc
     1021 cggccggcgc gacgcaggcg gcggctcgca cccgtaccct gcgcgtcacg cttggaggcc
     1081 ttgaattcga tttgcatgtg atgctcctga gtgggttgtc cctgcccgcg accaggcgga
     1141 gacggtggat gcccgtccga cgcggggccg gaagggcgaa agccttattc catgaacagc
     1201 gaggagaccg actcctcgtt gctgatgcgc aggatggtgt cggcgagcag cgaggccacc
     1261 gagatctggc ggatgcgcgg gcacgccttg gcgtcgtcgc gcagcggtat ggtgtcggtg
     1321 acgaccagct cgtcgagctc ggactcgttg atgcgcgaga ccgccgcgcc cgacagcacc
     1381 gcgtgggtgc agtaggacag cacacgcttg gcaccgtgct tcttgagcgc ggcggcagcc
     1441 ttgcacaggg tgccggcggt gtcgacgatg tcgtccatga tcacgcaggt gcggccctcg
     1501 acctcgccga tgatgttcat cacctcggag acgttggcct tggggcggcg cttgtcgatg
     1561 atcgccaggt cgcactccat gcgcttggcg aaggcgcgcg cgcgcaccac gccgccgacg
     1621 tcgggcgaga cgacgagcag gtcgtcgtac ttctgcttgt cgaggtcggc gagcagcacc
     1681 ggcgcggcgt acacgttgtc gaccgcgatg tcgaagaagc cctggatctg gtcggcgtgc
     1741 aggtccatgg tcagcaggcg ctggacgccc accgcctgca gcatgttggc gaccaccttg
     1801 gcggtgatcg gcacgcgcgc cgagcgcggg cggcgatcct ggcgggcgta gccgaaatag
     1861 gggatggcgg cggtgatgcg gccggcggag gcgcgcttga gggcgtcgac cagcaccagc
     1921 agttccatca gattgtcgtt ggtcggggag caggtgggct gcaggacgaa gacgtccttg
     1981 ccgcgcacgt tctcgagcag ttcgacattg acctcgccgt ccgagaagcg gccgacggtg
     2041 gccgagccga gggaaatccc caggcgtcgc gtcacgtcgg cgccaagctt ggggttggcg
     2101 ttgccggtga agaccatcag gctgccgtgg ggcatttctg ctactccgag tgccgtaagg
     2161 aacgacgagc gaatattcgg cgcgtaaaaa cgactcgggc agacccatgg tctgcccgtc
     2221 gttgtatggc tggggaggaa ggattcgaac cctcgaatgc cggaatcaaa atccggtgcc
//
LOCUS       DAOQNN010000487         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_38293_length_2270_cov_9.983296, whole genome shotgun sequence.
ACCESSION   DAOQNN010000487 DAOQNN010000000
VERSION     DAOQNN010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38293_length_2270_cov_9.983296"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PKV97_16460"
     CDS             <1..312
                     /locus_tag="PKV97_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNB07527.1"
                     /translation="VAGRDVVIFEDEIATAGTLVSSVDTLKAAGARSIHAAATHGVLC
                     GPAIERLRAAAIDSVVVTNTVALPEDKRLDKLAVLNVAPLFAAAIERIHSGQSVGALF
                     E"
     gene            complement(325..1236)
                     /locus_tag="PKV97_16465"
     CDS             complement(325..1236)
                     /locus_tag="PKV97_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNB07528.1"
                     /translation="MIVRPRPHWIHLLFVRRGSLLPRIFSQQLFVLLLSCAVVLAHGQ
                     LFHVKVTLTSAPFSLMGVALAIFLGFRINASYDRYWEARKFWGVVLVEARNLSRHALT
                     LTRADVDARPFVLGLIAFASTMRNQLRGRPRDEGLEGLLPDAVLGRVRSARFAPALVL
                     LWLGQWVRDRREAGCLPPMLAQHMEGSLDALSAALGGCERIANTPLPFTYTVILHRSS
                     YTYCMLLPFGLVDTTGWMTPLVVGFVSYTFFALEALSDEIEEPFGTMTNDLALDAIVA
                     SIDASLRELLGDTPPPAPQPDGDFVLN"
     gene            complement(1377..1850)
                     /locus_tag="PKV97_16470"
     CDS             complement(1377..1850)
                     /locus_tag="PKV97_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNB07529.1"
                     /translation="MEEMRHGPGKAHDNHLGDRVDDPLIDRLHWIIRQAIRVLAVLMV
                     AVILWTVADVVLVLYEKLSDPPFLLLDLNDIFVVFAAFLAVLIAIEIFVNITLYLRDD
                     VIHVKLVIATALMAIARKVIVLDLSTLEPMYLFAIGVIVLALGVTYWLVSARPGE"
     gene            complement(1931..>2270)
                     /locus_tag="PKV97_16475"
     CDS             complement(1931..>2270)
                     /locus_tag="PKV97_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNB07530.1"
                     /translation="TVLADGGLDRNEVDTMMHADLARRLGIGSVEFERILREYCDDLL
                     LSAHYLDGVRLKVADEVLDLLLEDIADPKLQQALLRTMQDIVSADGVETSAEVEVLAR
                     ALEKWGSRPH"
BASE COUNT          388 a          761 c          809 g          312 t
ORIGIN      
        1 gtggccgggc gcgacgtggt gatcttcgag gacgagatcg ccaccgccgg caccctggtg
       61 tcgtcggtgg acacgctcaa ggccgccggc gcgcgcagca tccacgccgc cgccacccac
      121 ggcgtgctgt gcggcccggc gatcgagcgc ctgcgcgcgg cggcgatcga ctcggtggtg
      181 gtcaccaaca ccgtggcgct gccggaagac aagcgtctcg acaagctcgc cgtgctcaac
      241 gtcgcaccgc tcttcgccgc ggcgatcgag cgcatccaca gcggccagag cgtgggcgcg
      301 ctgttcgagt gagcgggagc gcgctcagtt gagcacgaaa tccccatccg gctgcggcgc
      361 gggcggcggc gtgtcgccga gcagctcgcg caggctggcg tcgatggagg cgacgatggc
      421 atcgagcgcc aggtcgttcg tcatcgtgcc gaagggctcc tcgatctcgt cgctgagcgc
      481 ctcgagcgcg aagaaggtgt aggacacgaa acccaccacc agcggcgtca tccagcccgt
      541 cgtatccacc aggccgaagg gcagcagcat gcagtacgtg tacgagctgc ggtgcaggat
      601 caccgtgtag gtgaagggca gcggcgtgtt ggcgatgcgc tcgcagccgc caagcgcggc
      661 cgacagcgcg tcgagcgagc cctccatgtg ctgcgccagc atcggcggca ggcagcccgc
      721 ctcgcgccgg tcgcgcaccc attggccgag ccacagcagc accagcgcgg gtgcgaagcg
      781 ggcactccgc acgcgtccga gcaccgcgtc cggaagcagg ccttcgagcc cctcgtcgcg
      841 cggacggccg cgcagctggt tgcgcatcgt gcttgcgaag gcgatcaggc cgagcacgaa
      901 ggggcgggca tcgacgtcgg cgcgcgtcag ggtcagggca tgccgcgaca ggttgcgcgc
      961 ctcgaccagc acgacgcccc agaatttgcg cgcttcccag tagcggtcgt agctcgcgtt
     1021 gatgcgaaag ccgaggaaga tcgccagggc gacccccatc agcgagaacg gcgcggaggt
     1081 cagcgtgacc ttgacgtgga agagctggcc gtgcgcgagc accaccgcgc aggacagcaa
     1141 cagcacgaag agctgctgcg agaagatgcg cggcaacaag gagccgcggc ggacgaagag
     1201 aaggtgaatc cagtgcggac gagggcggac gatcacgacg ggcgggcgcg gggcgaggcg
     1261 caaaggcgtg gattctgaca gcttcgcggc gagggggagc aatttcgatc gcaaccgacg
     1321 ccctcggcgt ccggtcccgg ccgcgaccga cgggcctgcg gtgcccgcgt cccccctcac
     1381 tcgccgggcc gcgccgacac cagccagtag gtcacgccga gcgcgagcac gatcacgccg
     1441 atcgcgaaca ggtacatcgg ctccagcgtc gacaggtcga gcacgatcac cttgcgcgcg
     1501 atcgccatca gcgcggtggc aatcaccagc ttgacgtgga tgacgtcgtc gcgcaggtag
     1561 agcgtgatgt tgacgaagat ctcgatcgcg atcagcaccg ccaggaaggc ggcgaagacc
     1621 acgaagatgt cgttgaggtc gagcagcagg aagggcggat cggacagctt ctcgtagagc
     1681 acgaggacga cgtccgcgac ggtccacagg atcaccgcca ccatcagcac cgccagcacg
     1741 cggatcgcct ggcggatgat ccagtgcagg cggtcgatca ggggatcgtc gacacgatcg
     1801 cccagatggt tgtcgtgggc cttgccgggc ccgtgcctca tctcctccat gactgcctcc
     1861 gtggcggtgg tggcgagcgc gccggatcat gttccgaccc ggctcgccac cgggttcgtt
     1921 cgggcgtgcc ctagtgcggg cgcgagcccc atttttccag cgcgcgggcg agcacctcga
     1981 cctcggccga ggtctcgacc ccgtcggcgc tgacgatgtc ctgcatcgtg cgcaacaggg
     2041 cctgctgcag cttggggtcg gcgatgtcct cgagcagcag gtcgagcacc tcgtcggcca
     2101 ccttcaggcg cacgccgtcg aggtagtgcg cgctcagcag caggtcgtcg cagtattcgc
     2161 gcaggatgcg ctcgaactcc acgctgccga tgcccagcct gcgggcgagg tcggcgtgca
     2221 tcatcgtgtc gacctcgttg cggtccaggc cgccgtcggc gagcacggtg
//
LOCUS       DAOQNN010000488         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_38490_length_2264_cov_9.236306, whole genome shotgun sequence.
ACCESSION   DAOQNN010000488 DAOQNN010000000
VERSION     DAOQNN010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38490_length_2264_cov_9.236306"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PKV97_16480"
     CDS             complement(<1..991)
                     /locus_tag="PKV97_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNB07531.1"
                     /translation="MMRTLRLALRMMLRDLRAGELHLLGLAIMIAVASLTSVGFLADR
                     VGRALDREANQLLGGDLLLRADKPWPDAFIDEARSRGLQTARTVLFTSMASTAEGAAL
                     GGVKVVEEGYPLRGTIRLAPGPNQPDAPAGRAPEAGEVWLDERLFAELGVNTGDKVGL
                     GELEFRVGAMVSFEADRGANFFSLLPRAIFNLKDLEATGLIAEGSRATWRLHVAGAPQ
                     AVEAYEKWARANLGRGQRVETVDNARPEVRAALDQAQRFLRLAALLAVILAAVAVGLS
                     ARRFMARHLDGCAVMRVLGARQAQVLGIVVGEFLLFGLAAALAGTALGWAVQWG"
     gene            complement(1002..2174)
                     /locus_tag="PKV97_16485"
     CDS             complement(1002..2174)
                     /locus_tag="PKV97_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HNB07532.1"
                     /translation="MSLIESVRPLQSKLTAIRRDIHAHPELAFEERRTAELVARHLEA
                     LGIETHRGIGGTGVVGVVRGRRGLRAIGLRADMDALPITERNEFAHKSTIQGCMHACG
                     HDGHTTMLLGAAEALAARRDFDGTVYLIFQPAEEGEGGAPAMIADGLFERFPMEAVFG
                     MHNWPGMEAGSFAVHAGPVMASADRFDIRFTGVGAHAAMPHLGVDPVVAGAAFVQAAQ
                     TLVSRVLDPIDAGVVSVTQFHAGEAYNVIPDRAELCGTVRTFSAEVRDRLERGLRQVA
                     EGIAQSHGVQVDFEFRRGYPPTVNTVAEATLCAEAARAVAGPERVFTDRRPSMGAEDF
                     AYFLQEKPGAYVWVGNGPGEGGCMLHNPNYDFNDEVLPAGVAYWCALVQRLLGGVK"
BASE COUNT          324 a          831 c          801 g          308 t
ORIGIN      
        1 gcccccactg caccgcccag cccagcgcgg taccggcgag tgcggcggcc agcccgaaga
       61 gcaggaactc gcccaccaca atgcccagca cctgcgcctg acgcgcaccg agcacgcgca
      121 tcaccgcgca accgtcgagg tggcgtgcca tgaagcggcg cgcggacagg cccaccgcca
      181 ccgcggcgag gatcaccgcg agcagcgccg ccaggcgcag gaagcgctgc gcctggtcga
      241 gcgcggcacg cacctcgggg cgggcgttgt ccacggtctc gacgcgctgg ccgcggccga
      301 ggttcgcgcg cgcccacttc tcgtaagcct ccaccgcctg cggcgcgccg gccacgtgca
      361 agcgccaggt ggcgcggctg ccctcggcga tcaggccggt ggcctccaga tccttgaggt
      421 tgaagatcgc gcgcggcagc aggctgaaga agttggcgcc gcggtcggcc tcgaagctga
      481 ccatggcgcc gacgcggaac tcgagctcgc cgaggcccac cttgtcgccg gtgttcacgc
      541 ccagctcggc gaacaggcgc tcgtccagcc acacctcgcc cgcttcgggc gcgcgacctg
      601 cgggggcgtc gggctggttg ggaccgggcg ccagacggat ggtgccgcgc agcgggtagc
      661 cttcctccac caccttgacg ccgccgagcg ccgcgccctc ggcggtactc gccatgctgg
      721 tgaacagcac cgtcctggcg gtctgcaggc cgcggctgcg cgcctcgtcg atgaaggcgt
      781 cgggccacgg cttgtcggcg cgcagcagca ggtcgccacc gaggagctgg ttggcctcgc
      841 gatcgagcgc gcgcccaacg cggtcggcga ggaagcccac gctggtcagg ctcgccaccg
      901 cgatcatgat cgccagcccg agcaggtgca gctcgcccgc gcgcaagtcg cgcagcatca
      961 tgcgcagggc gagtcgaagc gtgcgcatca tcgggcggcg ctcacttcac gccgccgagc
     1021 aggcgctgca ccagcgcgca ccagtaggcc acgccggccg gcagcacctc gtcgttgaag
     1081 tcgtagttgg ggttgtgcag catgcagccg ccctcgcccg ggccgttgcc gacccacacg
     1141 taggcgcccg gcttctcctg caggaaataa gcgaaatcct ccgcacccat gctcgggcgg
     1201 cggtcggtga atacccgctc cggtcccgcc accgcgcgcg ccgcctcggc gcacagcgtg
     1261 gcctcggcga cagtgttgac ggtcggggga tagccgcggc ggaactcgaa atccacctgc
     1321 acaccatggc tctgcgcgat cccctcggcc acctggcgca ggccgcgctc gaggcggtcg
     1381 cgcacctcgg cggagaaggt gcgcaccgtg ccgcacagct cggcgcggtc ggggatcacg
     1441 ttgtaggcct cgccggcatg aaactgggtc accgacacca cgccggcgtc gatcggatcg
     1501 agcacgcggc tcaccagggt ctgcgccgcc tgcacgaagg ccgcgcctgc caccaccggg
     1561 tccacgccga ggtgcggcat cgccgcatgc gcgccgacgc cggtgaagcg gatgtcgaag
     1621 cggtcggcgc tcgccatcac cgggccggcg tgtacggcga agctccccgc ctccatgccc
     1681 ggccagttgt gcatgccgaa caccgcctcc atgggaaagc gctcgaagag tccgtcggcg
     1741 atcatcgccg gcgcgccgcc ctcgccttcc tcggcgggct ggaagatcag gtacaccgtg
     1801 ccgtcgaagt cgcgccgcgc ggccagcgcc tcggccgcgc cgagcagcat ggtggtgtga
     1861 ccgtcgtggc cgcaggcgtg catgcagccc tggatggtgg acttgtgcgc gaactcgttg
     1921 cgctctgtga tcggcaacgc atccatgtcg gcgcgcagcc cgatcgcacg cagcccgcgg
     1981 cggccgcgca ccacaccgac cacgccggtg ccgccgatgc cgcgatgggt ctcgatgccg
     2041 agcgcctcca gatggcgcgc caccagctcg gcggtgcggc gctcctcgaa ggcgagctcg
     2101 gggtgggcgt ggatgtcgcg gcggatcgcg gtcagcttgc tctgcagggg gcggacgctt
     2161 tcgatgaggc tcatggctgc accgtgttgt cgtggtttgt cgcgggtgtc cgagtgtagt
     2221 acaggcggac gcgggtggat ccttcggcgt cctcaatccg gttg
//
LOCUS       DAOQNN010000489         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_38491_length_2264_cov_8.107288, whole genome shotgun sequence.
ACCESSION   DAOQNN010000489 DAOQNN010000000
VERSION     DAOQNN010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38491_length_2264_cov_8.107288"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="PKV97_16490"
     CDS             complement(<1..1665)
                     /locus_tag="PKV97_16490"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HNB07533.1"
                     /translation="MGAPEHDLVSSLFRAYLRPRESLTVDAWADRCRVLAGETSAEPG
                     PWRTDRTPYLRQIMEDLSDDAQYEEVVLQFGTQLGKSESGLNWIGYCIDHDPGPMMLI
                     LPTVDIAKRFSKQRLAPMVRETPTLRARIRDSRSRDSGNTTLVKEFPGGLLVVTGANS
                     AAGLASMPSRVLYADEVDDYPDDVDGQGEPLGLATARQDTFARRKRLLSSSPKRPPGL
                     STIEARFHAGTRFRYEVPCPHCRAFQVLEWGGKEVPHGLKWIGNEPSTAHYVCRHCGV
                     VIEEHAKAAMLAGGRWAAENPLAAVRSYHLSSLYSPLGWLSWATIVREFLEAAAALVD
                     GKTAPMKTWRNTRLALTWAEPGARLQVHALKERAQRAGAHRLRDVPAPVAILTAGVDV
                     QDNRVEVSVWGWGPGEECALVDHQVIPGDPAQDDLWLRMDDYLATRFRHAGGGTIGVE
                     AVALDTGGHFTHTVYSYVRARSPGRRIDAGGQVWVQRVYAVKGQDRPGLPVKGKGTSV
                     DVNYRGKMVQRGVQLWMVGVNSAKDWLYARLRQERAGPGYVHLPADV"
     gene            complement(1652..2179)
                     /locus_tag="PKV97_16495"
     CDS             complement(1652..2179)
                     /locus_tag="PKV97_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07534.1"
                     /translation="MQPNLITPAEFARQIGVAKQTVHEAVKRGRLSVIDGKLDLEVAR
                     IQWDKNRSRRAPHSVEAAAPAGSPSGESTDGGAYWEAKTRREAAEASIAELKEAELRG
                     ELVRKAEVERAISSRLVALRESLEVLADRISALVAVESDAQVCRRLLRDEHRKALATF
                     TEQMLVADGAGDGRT"
BASE COUNT          310 a          873 c          730 g          351 t
ORIGIN      
        1 gacgtcggcc ggcaggtgaa cgtatcccgg ccccgcgcgc tcctggcgca gccgcgcata
       61 gagccagtcc ttggcactgt tcacgccgac catccacagc tgcacgcccc gctgcaccat
      121 cttgccgcgg tagttgacgt cgacgctcgt gcccttgccc ttcaccggca ggcccggccg
      181 gtcctggccc ttcacggcgt acacgcgctg cacccacacc tggccgccag cgtcgatgcg
      241 ccgacccggc gagcgcgcgc gcacgtacga gtacaccgtg tgcgtgaagt gcccgccggt
      301 gtccagtgcg accgcctcga cgccgatcgt cccgccgccg gcgtgccgga agcgcgtcgc
      361 gaggtagtcg tccatccgca gccagaggtc gtcctgcgcc gggtctccgg ggatcacctg
      421 gtggtcgacc agtgcgcact cctcgcccgg cccccagccc cacaccgaca cctcgaccct
      481 gttgtcctga acgtcgacgc ccgccgtcag gatcgccacc ggcgccggca cgtcgcgcag
      541 ccggtgcgcg ccggcgcgct gcgcccgttc cttcagcgca tgtacctgca gccgcgcgcc
      601 tggctcggcc caggtgagcg ccaaccgcgt gttcctccac gtcttcatcg gcgccgtctt
      661 gccgtccacg agcgcggccg cggcctcgag gaactcgcgc acgatcgtgg cccacgacag
      721 ccacccgagc ggcgagtaca ggctcgacag gtggtagctg cgcaccgccg ccagcgggtt
      781 ctccgcggcc caccggccgc cggccagcat cgcggctttc gcgtgctctt cgatcaccac
      841 gccgcagtgc cggcagacgt agtgcgcggt gctcggttcg ttgccgatcc acttcagccc
      901 gtgcggcacc tccttgccgc cccactcgag cacctggaac gcgcggcagt gcgggcatgg
      961 cacctcgtac cggaaccgcg tcccggcgtg gaaccgcgcc tcgatcgtcg agagcccggg
     1021 cgggcgcttc ggcgaggacg acagcagccg ctttcgccgc gcgaacgtgt cctgccgcgc
     1081 cgtcgccagc cccagcggct cgccctggcc gtcgacgtcg tccgggtagt cgtcgacctc
     1141 gtcggcgtac agcacgcgcg acggcatcga cgccaacccc gccgcactgt tcgcgccggt
     1201 gaccacgagc agcccgcctg ggaactcctt cacgagcgtc gtgttcccgc tgtcgcgcga
     1261 cctcgaatcc cggatgcgcg cacgcagcgt cggcgtctcg cgcaccatcg gcgcgagcct
     1321 ctgcttgctg aatcgcttgg caatgtcgac cgtcggcagg atcagcatca tcggccctgg
     1381 atcgtggtcg atacagtagc cgatccaatt cagcccggac tcgctcttgc ccaactgcgt
     1441 tccgaactgc agcaccacct cttcgtactg cgcgtcgtcc gacagatcct ccatgatctg
     1501 ccgcaggtac ggcgttcgat ccgtgcgcca cggccccggc tcggccgacg tctcgccggc
     1561 cagcacccgg cagcggtccg cccacgcatc caccgtgagc gactcgcgcg gccgaaggta
     1621 ggcgcggaac agcgacgaga caaggtcgtg ctcaggtgcg cccatcgccc gccccgtcag
     1681 cgaccagcat ctgctcggtg aacgtcgcga gcgccttgcg gtgctcgtcg cgcagcaagc
     1741 gccggcagac ctgcgcgtcg gactccaccg ccacgagcgc agagatccgg tcagcgagca
     1801 cctccagcga ctcgcgcagc gccaccaggc gcgaggagat cgcacgttcc acttctgcct
     1861 tgcgcacgag ctcgccgcgc agttccgcct ccttgagttc ggcgatcgag gcttcggcgg
     1921 cctcgcgccg ggtcttcgcc tcccagtagg cgccgccgtc ggtggactcg ccggaaggcg
     1981 accctgccgg cgctgcggcc tcgacggagt gcggcgcccg acggctgcgg ttcttgtccc
     2041 actggatgcg cgcgacctcg aggtcgagtt tcccgtcgat caccgacagc cggccgcgct
     2101 tgaccgcctc gtgcacggtc tgcttcgcca cgccgatctg gcgcgcgaac tcggccgggg
     2161 tgatcaggtt gggctgcacg gtcagggatg cggtggataa gtcaggatgg cggtcaggga
     2221 acctgacggg ctgggactag gttgcgatcg gggttcgcat tacc
//
LOCUS       DAOQNN010000490         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_38512_length_2263_cov_6.917120, whole genome shotgun sequence.
ACCESSION   DAOQNN010000490 DAOQNN010000000
VERSION     DAOQNN010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38512_length_2263_cov_6.917120"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /gene="gatB"
                     /locus_tag="PKV97_16500"
     CDS             complement(<1..295)
                     /gene="gatB"
                     /locus_tag="PKV97_16500"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763906.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HNB07535.1"
                     /translation="MSRSDWEVVIGLEVHAQLNTASKIFSGASTAFGAEPNRQASAVD
                     IALPGVLPVLNRGAVERAIRFGLAIGAHVAEKSVFARKNYFYPDLPKGYQISQY"
     gene            complement(333..848)
                     /locus_tag="PKV97_16505"
     CDS             complement(333..848)
                     /locus_tag="PKV97_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07536.1"
                     /translation="MSALSRPTLRPLHGRLAALAAAVLLSACALPVLPPSDAPISDEV
                     LASPSSAGKRLRPMPWRPLDVRADCRFRDEAGYTTRITLDIAQSKVNAFNADVDIPRR
                     GSCSFHGPFTQTREKPSVQLRAPDGCTVAIFEQGKAVTVGFANCASRCTRGASDYLWP
                     ILVDRTNGQCH"
     gene            complement(859..>2263)
                     /gene="gatA"
                     /locus_tag="PKV97_16510"
     CDS             complement(859..>2263)
                     /gene="gatA"
                     /locus_tag="PKV97_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNB07537.1"
                     /translation="AGLFLDRIDALNPALNAFITVDREGALAAARAADARIAAGSAGP
                     LTGIPLAHKDVFCTEGVLTTCGSKMLANFVSPYDAHVVSLLEAAGAVSLGKANMDEFA
                     MGSSNESSYFGAVKNPWNLGRIPGGSSGGSAAAVAARLAPIATGTDTGGSVRQPAAYC
                     GITGIKPTYGLVSRYGMIAYASSLDQGGAFGASAEDCALLLGAMTGFDARDSTSLERP
                     TEDYAAALAGPACARPLEGLRIGLPREFFAEGMSDDVRAAVEAAVEQYRVLGATTVEV
                     SLPNAKLAIPAYYVIAPAECSSNLSRFDGVRYGHRAADYGDLADMYAKSRAEGFGAEV
                     KRRILVGTYVLSHGYYDAYYLQAQRLRRLIAQDFQAALQHCDLIAGPTTPTTAWALGE
                     KADDPVQMYLSDIYTIAVNLAGLPGLSHPVGFGADGLPVGLQLIGDYFAESRLLHAAH
                     RFQHATDWHARRPACAA"
BASE COUNT          306 a          730 c          895 g          332 t
ORIGIN      
        1 cgtactggct gatctggtag cccttgggga ggtcggggta gaagtagttc ttgcgcgcga
       61 acacgctctt ttccgccacg tgggcgccga tcgccagccc gaagcggatc gcgcgctcga
      121 ccgcgccgcg gttgagcacc ggcagcacgc cgggtagggc gatgtccacc gcgctcgcct
      181 ggcggttggg ctcggcgccg aaggcggtgc tggcgccgga aaagatcttg ctggcggtgt
      241 tgagctgggc gtggacttcc agcccgatga cgacttccca atcggatcgg ctcatggtgt
      301 gttctcgtgt tgcgtgcagc cggccaccgg ggtcagtggc actggccgtt ggtccgatcg
      361 acgaggatcg gccacaggta atcggaggcg ccgcgggtgc agcggctggc gcagttggcg
      421 aagccgacgg tgacggcctt gccctgctcg aagatggcca ccgtgcagcc gtcgggcgcg
      481 cgcagctgca cgctcggctt ctcgcgcgtc tgcgtgaatg ggccgtggaa gctgcaactg
      541 ccgcggcgcg ggatgtcgac gtcggcgttg aaggcgttga ccttggactg cgcgatgtcg
      601 agcgtgatgc gcgtggtgta gccggcctcg tcgcggaagc ggcagtcggc acgcacgtcg
      661 agcggccgcc acggcatcgg gcgcaggcgc ttgccggccg agctcgggct ggccagcacc
      721 tcgtcgctga tcggcgcgtc ggaaggcggc agcaccggca acgcgcaggc cgacagcagc
      781 accgccgccg ccagtgcggc gaggcggccg tggagcgggc gcagcgtcgg gcgggacagg
      841 gcgctcactg cgtggaactc acgcggcgca cgccggccgg cgcgcgtgcc agtcggtggc
      901 gtgctggaag cggtgtgcgg cgtgcagcag gcgggattcg gcgaaataat cgccgatcag
      961 ctgcaggccc accggcaggc cgtcggcgcc gaagcccacc gggtgcgaca gtccgggcag
     1021 gccggcgagg ttcaccgcga tggtgtagat gtcggacagg tacatctgca ccgggtcgtc
     1081 ggccttctcg ccgagcgccc aggccgtggt cggcgtggtc ggtccggcga tcaggtcgca
     1141 gtgttgcagc gcggcctgga agtcctgcgc gatcaggcgg cgcaggcgct gcgcctgcag
     1201 gtagtaggca tcgtagtagc cgtgcgagag cacgtaggtg ccgaccagga tgcggcgctt
     1261 gacttcggcg ccgaagccct cggcacggct cttcgcgtac atgtcggcga ggtcgccgta
     1321 gtcggcggcg cggtggccgt agcgcacgcc atcgaagcga ctgaggttgc tcgagcattc
     1381 cgccggcgcg atcacgtagt aggccgggat cgccagcttc gcgttgggca gcgagacctc
     1441 gaccgtggtg gcgccgagca cgcggtactg ctcgaccgcc gcctcgaccg cggcgcgcac
     1501 gtcgtcggac atgccctcgg cgaagaactc gcgcggcagg ccgatgcgca ggccttcgag
     1561 cgggcgggcg caagcggggc cggcgagcgc ggcggcgtag tcctcggtcg ggcgctccag
     1621 gctggtggag tcgcgcgcgt cgaagccggt catcgccccg agcagcagcg cgcagtcctc
     1681 ggcgctggcg ccgaaggcgc cgccctggtc gagcgaggag gcataggcga tcatgccgta
     1741 gcggctcacc aggccgtagg tgggcttgat gccggtgatg ccgcagtagg ctgcgggctg
     1801 gcgcaccgag ccgccggtgt cggtgccggt ggcgatcggc gccaggcgcg ccgccaccgc
     1861 ggcggccgag ccgccggacg agccgccggg gatgcggccg aggttccacg ggttcttcac
     1921 cgcgccgaag tacgagctct cgttcgacga gcccatggcg aactcgtcca tgttggcctt
     1981 gcccaggctt accgcgccgg cggcctcgag caggctcacc acgtgggcgt cgtaggggct
     2041 gacgaagttg gccagcatct tcgagccgca ggtggtgagc acgccctcgg tgcagaacac
     2101 gtccttgtgc gcgagcggga tgccggtgag cggcccggcg ctgccggcgg cgatgcgcgc
     2161 gtcggccgcg cgcgcggcgg cgagcgcgcc ttcgcggtcg acggtgatga aggcgttgag
     2221 cgcggggttg agcgcgtcga tgcggtcgag gaacaggccc gcg
//
LOCUS       DAOQNN010000491         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_38589_length_2261_cov_6.487307, whole genome shotgun sequence.
ACCESSION   DAOQNN010000491 DAOQNN010000000
VERSION     DAOQNN010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38589_length_2261_cov_6.487307"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /gene="gorA"
                     /locus_tag="PKV97_16515"
     CDS             <1..1231
                     /gene="gorA"
                     /locus_tag="PKV97_16515"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HNB07538.1"
                     /translation="GGTCVNVGCVPKKVMWYAAGIAQALRDAPGYGFAEVAGRFDWAT
                     LKQRRDAYIERLNGIYAGMLDKSGVELKRGFARFSGPRVVEVEGEHFTAPHIVISTGG
                     RPALPDLPGAELGIDSDGFFALEAQPRRVAVVGAGYIAVELAGVFHGLGSEVSMLVRG
                     DRLLRPFDAMLRDELAAQMQEDGIALRFGTRARALRRQADGSLLVDCGEAGSLEVDAL
                     VWATGRQPNTDRLQLAAAGIEADTKGTIPTDAFQNTNVPGIYAIGDITGRAELTPVAI
                     AAGRRLALRLFRGAADSKLDYENIPTVVFSHPAIGTVGLTEDEARKRFTDVKVYTTRF
                     TAMYNALTEHRPKTSMKLVCAGAEERIVGAHVIGDGADEMLQGFAVAVKMGARKADFD
                     DTVAIHPTSAEEFVTMR"
     gene            1243..1452
                     /locus_tag="PKV97_16520"
     CDS             1243..1452
                     /locus_tag="PKV97_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07539.1"
                     /translation="MPMTYEQHLDEVATLLYERYDQSEKAAIKLVMAAQEDGFFSGHD
                     DDPSICTLERAQADARAVFRKYGKA"
     gene            complement(1449..1775)
                     /locus_tag="PKV97_16525"
     CDS             complement(1449..1775)
                     /locus_tag="PKV97_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNB07540.1"
                     /translation="MSDNAVHPFDARGVAKRFRHAAIFGALESLEDGEVMRFVNDHDP
                     LPLLNQIQQRYGNQVGIHYVARDPGNIVIDFTIQLGAPAAVGAPAAAVAGGGGCGGGG
                     GHGCCS"
     gene            1936..>2261
                     /locus_tag="PKV97_16530"
     CDS             1936..>2261
                     /locus_tag="PKV97_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNB07541.1"
                     /translation="MDSRPARRPFRHLGRLALPLVLAASLTLSGCGYNDFQRLDEQTR
                     AAWSEVLNQYQRRADLIPNLVETVKGEAAFEQETLTRVIEARARATSIQVTPGMLDDP
                     QAMERF"
BASE COUNT          385 a          807 c          746 g          323 t
ORIGIN      
        1 cggcggcacc tgcgtcaacg tcggctgcgt gcccaagaag gtgatgtggt acgccgccgg
       61 catcgcgcag gcgctgcgcg atgcccccgg atacggcttc gcggaggtcg ccgggcgctt
      121 cgactgggcc acgctcaagc agcgtcgtga tgcctacatc gagcgcctca acggcatcta
      181 cgccggcatg ctggacaagt ccggcgtcga gctgaagcgc ggcttcgcac gcttctccgg
      241 cccgcgggtg gtcgaagtcg aaggcgagca cttcaccgca ccccacatcg tgatctccac
      301 cggcggccgc cccgcgctgc ccgacctgcc cggcgccgag ctcggcatcg attcggacgg
      361 cttcttcgcc ctcgaggcgc agccgcgtcg cgtcgccgtg gtcggcgcgg gatacatcgc
      421 ggtggagctg gccggcgttt tccacggcct gggcagcgag gtctccatgc tcgtgcgcgg
      481 cgatcgcctg ctgcgcccct tcgacgccat gctgcgcgac gaactcgccg cgcagatgca
      541 ggaagacggc atcgcgcttc gcttcggcac ccgggcgcgc gcgctgcggc gccaggccga
      601 cggcagcctg ctcgtcgact gcggcgaggc cggcagcctc gaggtggacg cgctggtgtg
      661 ggccacaggc cgccagccca acaccgaccg cctgcaactc gccgcggccg gcatcgaggc
      721 cgacaccaag ggtacgatcc ccaccgacgc gttccagaac accaacgtgc ccggcatcta
      781 cgcgatcggc gacatcaccg gccgcgccga gctcaccccg gtggcgatcg ccgccggccg
      841 tcgcctcgcc ctgcgcctgt tccgcggcgc ggccgacagc aagctcgact acgagaacat
      901 ccccaccgtg gtgttcagcc acccggccat cggcaccgtc ggcctcaccg aggacgaggc
      961 gcgcaagcgc ttcaccgacg tcaaggtcta caccacgcgc ttcaccgcca tgtacaacgc
     1021 gctcaccgag catcgcccga agacgtcgat gaagctggtg tgcgccgggg cggaggagcg
     1081 catcgtcggc gcccatgtga tcggcgacgg cgccgacgag atgctgcagg gcttcgcggt
     1141 ggcggtgaag atgggcgcgc gcaaggcgga cttcgacgac acggtcgcga tccacccgac
     1201 cagcgccgaa gaattcgtca ccatgcgcta agggagcgag cgatgccgat gacttacgag
     1261 caacacctgg acgaggtcgc cacgctttta tacgagcgct acgaccagag cgaaaaggcg
     1321 gccatcaagc tggtgatggc cgcccaggag gacgggttct tcagcgggca cgacgacgat
     1381 ccgtcgatat gcaccctgga acgcgcgcag gccgatgccc gcgcggtctt ccgcaagtac
     1441 ggaaaggctt aggagcagca gccgtggccg ccaccgccac cgcaaccgcc gccaccggcg
     1501 accgcggcag cgggcgcgcc caccgcagcc ggcgcgccga gctggatggt gaagtcgatg
     1561 acgatgttgc ccgggtcgcg ggcgacgtag tggatgccga cctggttgcc gtagcgttgc
     1621 tggatctggt tcagcagcgg cagcggatcg tggtcgttga cgaagcgcat gacctcgccg
     1681 tcctcgagcg actcgagcgc accgaagatg gcggcatggc ggaagcgctt ggcaacgccg
     1741 cgcgcatcga aggggtgaac agcgttatcg gacatcttgg atcttccatt gggggtccgg
     1801 tctcgaacct gcggccggaa tcgcagctcg cgaatcctag taccgcgcgc aggcggaatc
     1861 cttgagcaag gacaaaaacc ccgtgcgcac tgctagagtg aagatctttc caccggcatc
     1921 ttccggagaa gcgaaatgga ttcccgcccc gctcgtcggc ctttccgtca cctcggccgc
     1981 ctcgcgctac cgctcgtcct cgccgcctcg ctgacactgt ccggctgcgg ttacaacgac
     2041 ttccagcgcc tcgacgaaca gacccgcgcc gcgtggtcgg aggtgctcaa ccagtaccag
     2101 cgccgcgccg atctcatccc caacctggtc gagacggtca agggcgaagc cgccttcgag
     2161 caggagacgc tgacccgcgt gatcgaggcg cgcgcccgcg ccacatcgat ccaggtcacc
     2221 cccgggatgc tcgacgaccc gcaagcgatg gagcgcttcc a
//
LOCUS       DAOQNN010000492         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_38836_length_2253_cov_11.812557, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000492 DAOQNN010000000
VERSION     DAOQNN010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38836_length_2253_cov_11.812557"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2253
                     /gene="carB"
                     /locus_tag="PKV97_16535"
     CDS             <1..>2253
                     /gene="carB"
                     /locus_tag="PKV97_16535"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNB07542.1"
                     /translation="ATADVTYIEPITWQVVEKIIAKERPDAVLPTMGGQTALNCALDL
                     HKHGVLAKYGVEMIGANEHAIEKAEDRLKFKDAMTKIGLGSAKSGIAHSMEEAWAVQK
                     SIQAEIGGSGFPMVIRPSFTLGGTGGGIAYNPEEFEEICKRGIDLSPTNELLIEESLI
                     GWKEYEMEVVRDRADNCIIVCSIENLDPMGIHTGDSITVAPAQTLTDREYQLLRNASI
                     AILREIGVDTGGSNVQFSINPRDGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLA
                     VGYTLDELKNDITGGATPASFEPSIDYVVTKIPRFAFEKFPAADPHLTTQMKSVGEVM
                     AMGRTFQESFQKALRGLETGIDGLTERSTDREEIVEEIGEPGPERILFVGDAFRIGMT
                     LDEIFEETAIDPWFLAQIEQLIQVEQSLKGRQLDGLTAQELRFLKKKGFSDRRLAKLL
                     GTDQHAVRALRHALGVRPVYKRVDTCAAEFATQTAYLYSCYEGEDGECEAAPSDRKKI
                     MVLGGGPNRIGQGIEFDYCCVHAALAMREDGYETIMVNCNPETVSTDYDTSDRLYFEP
                     VTLEDVLEIVAKEQPLGVIVQYGGQTPLKLALDLERNGVPIIGTTPDSIDIAEDRERF
                     QQLLHTLGLKQPPNRTARTEEAAMAMAHEIGYPLVVRPSYVLGGRAMEIVHGDRDLER
                     YMREAVRVSEKSPVLLDRFLDDAVEVDVDCISDGVDTMIGGIMEHIEQAGVHSGDSAC
                     SLPPYTLSAV"
BASE COUNT          411 a          768 c          759 g          315 t
ORIGIN      
        1 cggccacggc cgacgtcacc tacatcgagc ccatcacctg gcaggtggtc gagaaaatca
       61 tcgccaagga gcgcccggat gcggtgctgc ccaccatggg cggccagacc gcgctgaact
      121 gcgcgctgga cctgcacaag cacggcgtgc tggcgaagta cggcgtcgag atgatcggcg
      181 ccaacgagca cgccatcgag aaggccgagg accgcctgaa gttcaaggac gcgatgacca
      241 agatcggcct gggctcggcc aagtcgggca tcgcgcactc gatggaagaa gcctgggcgg
      301 tgcagaagag catccaggcc gagatcggtg gctcgggttt cccgatggtg atccgcccca
      361 gcttcacgct cggcggcacc ggcggcggca tcgcctacaa cccggaggag ttcgaggaga
      421 tctgcaagcg cggcatcgac ctctccccga ccaacgagct gctgatcgag gagtcgctga
      481 tcggctggaa ggagtacgag atggaggtgg tccgcgaccg ggcggacaac tgcatcatcg
      541 tctgctcgat cgagaacctc gacccgatgg gcatccacac cggtgactcg atcaccgtgg
      601 cgccggccca gaccctgacc gaccgcgagt accagctgct gcgcaacgcc tcgatcgcca
      661 tcctgcgcga gatcggcgtg gacaccggcg gctcgaacgt gcagttctcg atcaacccgc
      721 gcgacgggcg catggtcgtc atcgagatga acccgcgcgt gtcgcgctcc tccgcgctgg
      781 cctccaaggc cacgggcttc ccgatcgcca agatcgccgc caagctggcg gtgggctaca
      841 cgctcgacga gctgaagaac gacatcaccg gcggcgcgac cccggcgtcc ttcgagccct
      901 cgatcgacta cgtcgtcacc aagatccctc gcttcgcctt cgagaagttc cccgcggccg
      961 acccgcacct gaccacgcag atgaagtcgg tgggcgaggt gatggccatg ggccgcacct
     1021 tccaggagtc cttccagaag gcgctgcgcg gcctggagac cggcatcgac ggcctgaccg
     1081 agcgttcgac cgaccgcgag gagatcgtcg aggagatcgg cgagccgggg ccggagcgca
     1141 tcctcttcgt cggcgacgcc ttccgcatcg gcatgacgct ggacgagatc ttcgaggaaa
     1201 ccgcgatcga cccgtggttc ctggcgcaga tcgagcagct gatccaggtg gagcagtcgc
     1261 tcaagggccg ccagctcgac ggcctgaccg cgcaggagct gcgcttcctg aagaagaagg
     1321 gcttctccga ccgccgcctc gccaaactgc tgggcaccga ccagcacgcc gtgcgcgcgc
     1381 tgcgccacgc gctgggcgtg cgcccggtct acaagcgggt ggacacctgc gcggcggagt
     1441 tcgccaccca gaccgcctac ctgtactcct gctacgaagg cgaggacggc gagtgcgagg
     1501 ccgcgccgtc ggaccggaag aaaatcatgg tgctgggcgg tggcccgaac cgcatcggcc
     1561 agggcatcga gttcgactac tgctgcgtcc atgcggcgct ggcgatgcgc gaggacggct
     1621 acgagaccat catggtcaac tgcaacccgg agaccgtctc caccgactac gacaccagcg
     1681 accgcctcta cttcgagccg gtgacgctgg aggacgtgct ggagatcgtg gccaaggagc
     1741 agccgctggg cgtgatcgtc cagtacggcg gccagacgcc gctgaagctg gcgctggacc
     1801 tggagcgcaa cggcgtgccc atcatcggca ccacgccgga ctcgatcgac atcgccgagg
     1861 accgcgagcg cttccagcag ctgctgcaca ccctcggcct gaagcagccg cccaaccgca
     1921 ccgcccgcac cgaagaggcg gcgatggcga tggcgcacga gatcggctac ccgctggtgg
     1981 tgcgcccgag ctacgtgctg ggcggccgtg cgatggagat cgtgcacggc gaccgtgacc
     2041 tcgagcgcta catgcgcgaa gcggtgcgcg tgtccgagaa gtcgccggtg ctgctggacc
     2101 gcttcctgga cgacgccgtc gaggtggacg tcgactgcat cagcgacggc gtcgacacga
     2161 tgatcggcgg catcatggag cacatcgagc aggcgggcgt gcactccggc gactcggcct
     2221 gctcgctgcc gccgtacacg ctgagcgccg tgc
//
LOCUS       DAOQNN010000493         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_38858_length_2252_cov_16.985890, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000493 DAOQNN010000000
VERSION     DAOQNN010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38858_length_2252_cov_16.985890"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2252
                     /locus_tag="PKV97_16540"
     CDS             <1..>2252
                     /locus_tag="PKV97_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HNB07543.1"
                     /translation="LERALRDNGGDRLEELAAEIRRLEQEKHQRQQRADRHTALLARI
                     DEPPPADETAFLAQQQGIAQRAEGLRERIADLDNREREEDFALRKGREEHAALTEEIE
                     SLERRRSNIDAAQVRIRDGLCSALAIGEDELPFAGELIQVREDEREWQGAAERLLRGF
                     GLALLVPDTHYRAVADWVDRQHLGARLVYFRVRPRKAQPGAGLHPQSLVRKLVIKPDS
                     PHYEWLEQELRQRFDVACCDTGEQFRREERAITRAGQIKEPSGRHEKDDRSRIDDRSR
                     YVLGWSNADKLRALRDQRDRLEARLATLGQRIGEIQQERNELRGRLDALSKLEEVTRF
                     ADIDWMSLARQIAALTEECATLEAASDTLQELGRQLKAAQARLTEVERQRDAARDKRS
                     AVATKQEAAQTLRDQARSVWADAPLTEAQIERLDAWRSQALDEHQLTVESCDNREQEL
                     RKWLQERIDAEDKRLARLAERIVNAMRGFKEEFKAETVEMDVSLAALAEYARLLTGLK
                     GDDLPRFEARFKELLNENTIREIANFNGQLYRERETIRERVERINQSLAGIDYNPGRF
                     IRLLAQPSPDAEIRDFQQDLRACTEGALTGSADEQYSEAKFVQVKAIIDRFRGREGLT
                     DADRRWTAKVTDVRQWFSFSASERWRADGAEHEHYADSGGKSGGQKEKLAYTVLAASL
                     AYQFGLEWGAVRSRSFRFVVIDEAFGRGSDESAQYGLRLFQQLNLQLLIVTPLQKIHI
                     IEPFVASVGFV"
BASE COUNT          395 a          761 c          804 g          292 t
ORIGIN      
        1 ctggagcggg ccctgcgcga caacggcggc gaccgcctgg aggagttggc ggcggaaatt
       61 cgccgtctgg agcaggagaa gcaccagcgc cagcagcgcg ccgaccgcca cacagcgctg
      121 ctcgcgcgca tcgacgagcc gccgccagcc gatgaaaccg cctttctcgc ccagcagcag
      181 ggcatcgccc agcgcgccga aggcctgcgc gagcgcatcg ccgacctcga caaccgcgaa
      241 cgcgaggagg acttcgcctt acgcaagggg cgcgaagagc acgccgccct gaccgaggag
      301 atcgaaagtc ttgagcggcg ccggagcaac atcgacgccg cccaggtccg catccgcgac
      361 gggttgtgtt cggcgctggc gatcggcgag gacgaactgc ccttcgccgg cgagctgatc
      421 caggtgcgcg aggacgagcg ggagtggcaa ggcgccgccg agcggctgct gcgcggcttc
      481 ggcctcgcgc tgctggtgcc ggacacgcac taccgagccg tcgccgactg ggtggaccgt
      541 cagcacctgg gcgcgcggct ggtgtacttc cgcgtgcggc cgcgcaaggc ccagccgggc
      601 gcgggcctgc atccgcagtc cttggtgcgc aagctcgtca tcaagccgga ttcgccgcac
      661 tacgagtggc tggagcagga gctgcgccag cgcttcgatg tggcctgctg cgatacgggc
      721 gagcagtttc gccgcgagga gcgcgccatc acccgcgccg ggcagatcaa ggagccgagc
      781 gggcgccacg agaaggacga ccgcagccgt atcgacgacc gcagccgtta cgtgctcggc
      841 tggagcaatg ccgacaagct gcgcgccctg cgcgaccagc gggaccgtct ggaggcaaga
      901 ctcgccacgc tggggcagcg catcggcgag atccagcagg agcgcaacga actgcggggc
      961 cgactggatg cactcagcaa gctggaggag gtcacgcgct ttgcggatat cgactggatg
     1021 agtctggccc gccagatagc cgcgctcacc gaggaatgtg cgacgttgga ggccgcctcc
     1081 gacacgctgc aagagctggg tcgccagctc aaggcggcgc aagcgcggct cacggaggtg
     1141 gaacgccagc gcgacgcggc gcgcgacaag cgctcggcgg tagccaccaa gcaagaggcg
     1201 gcgcaaacgc tgcgtgatca ggcccgctcg gtttgggccg atgcgccgct gaccgaggcg
     1261 cagatcgagc gtctggacgc ctggcgttcg caggcgctgg acgagcatca actcacggtc
     1321 gagtcctgcg acaaccgcga gcaggagctg cgcaagtggc tgcaggagcg catcgacgcg
     1381 gaggacaagc gcctggcccg tctggccgag cgcatcgtca atgccatgcg cggcttcaag
     1441 gaggagttta aggccgagac cgtggagatg gacgtgagcc tcgcggcgct ggcggagtac
     1501 gcgcggctgc tcacgggcct caagggcgac gacctgccgc gcttcgaggc gcgcttcaag
     1561 gagctgctca acgagaacac catccgcgag atcgccaact tcaacggcca gctctaccgc
     1621 gagcgcgaga cgatccggga gcgggtcgag cgcatcaacc agtcgctggc cggcatcgac
     1681 tacaacccgg ggcgcttcat ccggctgctg gcgcagccga gccccgatgc cgagatccgc
     1741 gacttccagc aggacctgcg cgcctgcacc gagggcgcgc tcaccggctc ggccgacgag
     1801 cagtactcgg aagccaagtt cgtgcaggtc aaggccatca tcgaccgctt ccgcggccgc
     1861 gagggcctca cggacgccga ccggcgctgg accgccaagg tcaccgacgt gcggcagtgg
     1921 ttttcgttct cggccagcga gcgctggcgc gccgatggcg ccgagcacga gcactacgcg
     1981 gactcgggcg gcaagtcggg cggccagaag gagaagctgg cctacaccgt gctggccgcg
     2041 agcctggcct accagttcgg gctggagtgg ggcgcggtgc gctcgcgctc gttccgcttc
     2101 gtggtgatcg acgaggcctt cgggcgcggc tcggacgaat ccgcccagta cgggctgcgc
     2161 ctgttccagc agctgaacct gcaactgctg atcgtcacgc cgctgcagaa gatccacatc
     2221 atcgagccct tcgtcgccag cgtcggcttc gt
//
LOCUS       DAOQNN010000494         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_39088_length_2244_cov_8.219735, whole genome shotgun sequence.
ACCESSION   DAOQNN010000494 DAOQNN010000000
VERSION     DAOQNN010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39088_length_2244_cov_8.219735"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKV97_16545"
     CDS             <1..299
                     /locus_tag="PKV97_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HNB07544.1"
                     /translation="VPPVSLSSAATRWGSCSTRSGIRLHWRLIHLEPALIDYVVAHEV
                     AHLAEMNHSPRFWAVVERLYPDWRAARAALRSAAATLPVIATAAADRGAFTRED"
     gene            327..713
                     /gene="gloA"
                     /locus_tag="PKV97_16550"
     CDS             327..713
                     /gene="gloA"
                     /locus_tag="PKV97_16550"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HNB07545.1"
                     /translation="MRILHTMLRVGDLERSLAFYTEVLGMRLLRRQDYPDGKFTLAFV
                     GYQDEAHGAVLELTHNWGVDKYELGTAYGHIALEVADAKKACDDIRARGGKVVREAGP
                     MKHGTTVIAFVEDPDGYKVELIERKG"
     gene            complement(746..1837)
                     /locus_tag="PKV97_16555"
     CDS             complement(746..1837)
                     /locus_tag="PKV97_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB07546.1"
                     /translation="MKFPLDRPMQVAVVDDTQLNLFLIGKLVDRMQDARAHNFERPTA
                     ALEWCLANAPDLIIVDYMMPEMDGLEFIRSIRACRDRDDLPILMVTASHERDVRYAAL
                     DCGASDFLTKPVDAREFEARVRNMLKLRQAHLANEHRAETLAAEVRRATADILDRERE
                     TITRLSRAAEFRDPETGAHIQRMSHYSALIARRLGLGDTFAETLLMAAPMHDVGKLGI
                     PDTILLKPGRLTADEFAVMKRHPQIGHDILKGSSSSILRLGATIALTHHEKFDGSGYP
                     QGLSGQSIPMEGRIVAVADVFDALTSARPYKPAWPMSRAIALLREGRGTHFDPTCVDA
                     LFHTWEDVLAVQSRYQDEHAPEPPVSPPC"
     gene            complement(1915..>2244)
                     /locus_tag="PKV97_16560"
     CDS             complement(1915..>2244)
                     /locus_tag="PKV97_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNB07547.1"
                     /translation="KSSPAIGSGIRPNCEAYGYLLDSSGNCQYIEAYNREVAAHPEAK
                     KVKVFDKTCLCTHMRNFDCWTCGHYTYRLKDTTVREDGGDYRILSAEHVFKDYQFSTE
                     GRIALPD"
BASE COUNT          338 a          700 c          826 g          380 t
ORIGIN      
        1 cggttccgcc ggtgagcttg tcgagcgcgg ctacgcgctg gggtagctgc agcacccgct
       61 cgggcatccg cctgcactgg cggctgatcc atctcgagcc ggcgctgatc gactacgtgg
      121 tcgcccacga ggtcgcgcac ctggccgaga tgaatcactc gccgcgcttc tgggcggtcg
      181 tcgagcgtct ttacccggac tggcgtgccg cgcgcgccgc gctgcgcagc gcggcggcga
      241 cgctgccggt gatcgcgacc gccgccgccg accgcggcgc cttcacccgc gaagattgaa
      301 accaccacga tacaggaaga caccccatgc gaatccttca caccatgctg cgcgtcggcg
      361 atctcgagcg ctcgctcgcc ttctacaccg aggtgctggg catgcgcctg ctgcgccgcc
      421 aggactatcc ggacggcaag ttcaccctcg ccttcgtcgg ctaccaggac gaggctcacg
      481 gagcggtgct ggagctgacc cacaactggg gcgtggacaa gtacgaactc ggcaccgcat
      541 acggccacat cgccctagag gtcgcggacg cgaagaaggc ctgcgacgac atccgcgcgc
      601 gcggcggcaa ggtggtgcgc gaggcgggac cgatgaagca cggcaccacc gtgatcgcct
      661 ttgtcgagga tcctgacggc tacaaggtcg aactgatcga gcgcaagggc tgacgcaggc
      721 gcccccgggg gtgggggcgc aggcctcagc aggggggcga gacgggcggc tccggggcgt
      781 gctcgtcctg gtagcgcgac tgcacggcga gcacgtcctc ccaggtgtgg aagagcgcgt
      841 cgacgcaggt cgggtcgaag tgggtgccgc gcccctcgcg cagcagcgcg atcgcccgcg
      901 acatcggcca tgccggcttg tagggccgtg cggaggtgag cgcatcgaac acgtcggcga
      961 ccgcgacgat gcggccctcc atcgggatcg actggccgga gagtccctgc gggtagccgc
     1021 tgccgtcgaa cttctcgtgg tgggtgaggg cgatggttgc gcccaggcgc aggatgctgg
     1081 atgacgaacc cttgaggatg tcgtggccga tctgcgggtg gcgcttcatc accgcgaact
     1141 cgtccgcagt cagtcggccg ggcttgagca ggatggtatc ggggatgccg agcttgccca
     1201 cgtcgtgcat cggtgcggcc atcagcaggg tctcggcgaa ggtgtctccc aggcccaggc
     1261 ggcgggcgat cagggccgaa tagtgcgaca tgcgctggat gtgggcgccg gtctcgggat
     1321 cgcggaactc ggccgcacgc gacagccggg tgatggtctc gcgctcgcgg tcgaggatgt
     1381 cggcggttgc ccggcgcacc tcggcggcga gggtctcggc gcggtgctcg ttggcgaggt
     1441 gggcctggcg cagcttgagc atgttgcgca cgcgcgcttc gaattcgcgc gcatcgaccg
     1501 gcttggtgag gaagtcgctc gcgccgcagt cgagtgccgc gtagcgcacg tcgcgctcgt
     1561 ggctggcggt gaccatcagg atgggcaggt cgtcgcggtc gcggcaggcg cggatgctgc
     1621 ggatgaattc cagcccatcc atctcgggca tcatgtagtc gacgatgatg agatcgggcg
     1681 cattggcgag gcaccactcg agcgcggcgg tggggcgctc gaagttgtgt gcacgcgcat
     1741 cttgcatgcg gtcgacgagc ttgccgatca ggaacaggtt gagctgcgtg tcgtcgacca
     1801 ctgcgacctg catcggacgg tcgagtggga atttcatccg atgagtatcg gaggccttgg
     1861 gcctcgcacc gaggaactat tgcgcggacg gcatcgcatc cgcgcatcct cggttcagtc
     1921 gggcagggcg atgcggcctt cggtgctgaa ctggtagtcc ttgaacacgt gctcggcgga
     1981 caggatgcgg tagtcgccgc cgtcctcgcg cacggtggtg tccttgagcc ggtaggtgta
     2041 gtggccgcag gtccagcagt cgaagttgcg catgtgcgtg cacaggcagg tcttgtcgaa
     2101 gaccttgacc ttcttcgcct cggggtgcgc ggcaacctcg cggttatagg cctcgatgta
     2161 ctggcagttg ccgctggagt cgagcaggta gccgtaggcc tcgcagttgg ggcggatgcc
     2221 gctgccgatc gccgggctgc tctt
//
LOCUS       DAOQNN010000495         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_39158_length_2242_cov_8.802469, whole genome shotgun sequence.
ACCESSION   DAOQNN010000495 DAOQNN010000000
VERSION     DAOQNN010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39158_length_2242_cov_8.802469"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PKV97_16565"
     CDS             complement(<1..223)
                     /locus_tag="PKV97_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNB07548.1"
                     /translation="MNDDNARLRAAIDTLRVPIYMKDADGRYVYANDLACSLLKRSRD
                     DVVGRCDQDLFPPDQAEVLRRNDLQVIATA"
     gene            439..1977
                     /locus_tag="PKV97_16570"
     CDS             439..1977
                     /locus_tag="PKV97_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HNB07549.1"
                     /translation="MTTLRLILGDQLHAAHSWFRAPRADALYLMMEVRSETDYVRHHA
                     QKVLAIFAAMRAFAAALQAAGHRVRYLKIGDADNRQSFAANLAQVAAECGATRFERME
                     ADEWRVERLLDEAAQTLGLPQAVVGSEHFLAERAELAQRFAAKVPRMEFFYRDLRRRH
                     RILVDADDVPVGGVWNFDAQNREKWPGDPPAPAWPWRGHDLRALWDEIVAAGARTIGA
                     PHADQLCWPITRREARAGLAHFIEHALPWFGPYQDAMSTRSTTLFHSGLSFALNVKLL
                     HPREVIDAAVAAWQAGKVELASCEGFVRQILGWREFVRGVYWARMPGYAQANALEARR
                     PLPAWYWSGDTKMACLRHAIAQSLETAYAHHIQRLMITGNFALLAGCDPDAVDAWYLG
                     IYIDAFEWVEMPNTRGMSQFADGGVIASKPYAGAASYIGKQSDYCKGCAYDPRRRHGA
                     SGTKSACPFNSLYWDFLLRHEARFARNPRMAMPYKAWAKMDAGERAATLAQAAHWLAR
                     LDEL"
     gene            complement(2044..>2242)
                     /gene="tdh"
                     /locus_tag="PKV97_16575"
     CDS             complement(2044..>2242)
                     /gene="tdh"
                     /locus_tag="PKV97_16575"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309492.1"
                     /note="converts threonine and NAD to
                     1,2-amino-3-oxobutanoate and NADH; functions in threonine
                     catabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNB07550.1"
                     /translation="LEIKGIYGREMFETWYKMVAMLQSGLDLSPIITHHYPVEEFKTG
                     FETMLSGNSGKVILDWEALAA"
BASE COUNT          347 a          803 c          758 g          334 t
ORIGIN      
        1 tggcggtcgc gatgacctgc aggtcgttgc ggcgcaggac ctcggcctga tccggcggaa
       61 aaagatcctg gtcgcagcgg ccgacgacat cgtcacgcga gcgtttcagc agcgagcacg
      121 cgagatcgtt ggcgtagaca tagcgcccgt ccgcatcctt catgtagatc gggacgcgca
      181 gcgtgtcgat ggccgccctc aggcgagcgt tgtcgtcgtt catgtgcagc cctgttgtcc
      241 ttgatgatcg cggccggtcg agcagcccga ctcgggcaca acagtgtccg gatcaagcgc
      301 accgactgca accacaaaat gcatacggac ttcccccgcg ggcagggcag cgggcacggt
      361 cgcggcccct cgccctagaa tgacggcgcg ccgaaccgac gcacgccgac accacaagct
      421 cgcccgccct tcgtcacgat gaccactctc cgcctgatcc tcggcgacca gctccacgcc
      481 gcccactcct ggttccgcgc cccgcgcgcc gacgcgctgt acctcatgat ggaggtgcgc
      541 agcgagaccg actacgtccg ccaccatgcg cagaaggtgc tggcgatctt cgccgcgatg
      601 cgcgccttcg ccgccgcgct gcaggccgcc ggtcatcgcg tgcgctacct gaagatcggc
      661 gacgccgaca accgccagtc cttcgccgcc aacctggcgc aggtggcggc cgagtgcggc
      721 gcgacccgct tcgagcgcat ggaggccgac gagtggcggg tggagcgcct gctggacgag
      781 gctgcgcaga cgctcggcct gccgcaggcg gtggtcggca gcgagcactt cctcgccgag
      841 cgtgccgaac tcgcgcagcg ctttgccgcc aaggtgccgc gcatggagtt cttctaccgc
      901 gacctgcgcc gccgccaccg catcctggtc gacgccgacg acgtccccgt gggcggggtg
      961 tggaacttcg acgcgcagaa ccgcgagaaa tggccgggcg acccgcccgc gccggcatgg
     1021 ccctggcgcg gccacgacct gcgtgcgctg tgggacgaga tcgtcgcggc gggcgcgcga
     1081 accatcggtg cgccccatgc cgaccagctg tgctggccga tcacccggcg cgaggcgcgt
     1141 gccgggctgg cgcacttcat cgagcacgcc ctgccctggt tcggccccta ccaggacgcg
     1201 atgagcacgc gctcgaccac gctgttccac tccgggctgt cgttcgcgct caacgtcaag
     1261 ctgctgcacc cgcgcgaggt catcgacgcc gcggtcgccg cctggcaggc gggcaaggtg
     1321 gagctggcga gctgcgaggg cttcgtgcgc cagatcctgg gctggcggga gttcgtgcgc
     1381 ggcgtgtatt gggcgcgcat gccgggctat gcgcaggcca atgcgctcga agcccggcgc
     1441 ccgctgccgg cctggtactg gagcggcgac acgaagatgg cctgcctgcg ccacgccatc
     1501 gcccaatcgc tggagaccgc ctacgcccac cacatccagc gcctgatgat caccggcaac
     1561 ttcgccctgc tggcgggctg cgacccggac gcggtggacg cctggtacct gggcatctac
     1621 atcgacgcct tcgagtgggt ggagatgccc aacacccgcg gcatgagcca gttcgccgac
     1681 ggcggcgtga tcgcgagcaa gccctacgcc ggtgcggcga gctacatcgg caagcagtcg
     1741 gactattgca agggctgcgc ctacgacccc aggcgccgcc acggcgccag cggcacgaag
     1801 tccgcctgcc ctttcaacag cctgtactgg gacttcctgc tgcgacacga ggcgcgcttc
     1861 gcgcgcaacc cgcgcatggc gatgccgtac aaggcgtggg cgaagatgga cgcgggcgag
     1921 cgcgccgcca cgctggcgca ggccgcgcac tggctggcgc ggctggacga actctgagcg
     1981 ggcccgccaa cggacagagc ccgggcctgc gcgggctctg tcctgtttgc cacagcgcaa
     2041 ggatcaggcg gccagcgctt cccagtccag gatcaccttg ccgctgttgc ccgagagcat
     2101 ggtctcgaag ccggtcttga actcctcgac cgggtagtgg tgggtgatga tcgggctcag
     2161 gtccagcccg ctctgcagca tggcgaccat cttgtaccag gtctcgaaca tctcgcggcc
     2221 gtagatgccc ttgatctcca gg
//
LOCUS       DAOQNN010000496         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_39820_length_2223_cov_5.754151, whole genome shotgun sequence.
ACCESSION   DAOQNN010000496 DAOQNN010000000
VERSION     DAOQNN010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39820_length_2223_cov_5.754151"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1114)
                     /locus_tag="PKV97_16580"
     CDS             complement(212..1114)
                     /locus_tag="PKV97_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNB07551.1"
                     /translation="MSKVFISYRRDDSAGFAGRLADALERRFGADSVFRDVDDISAGA
                     DFGVVIEQALQQVQAVLVVIGTRWLDARDGRGRRLDDPGDYVRREVELALASGKPLIP
                     VLVGGATMPAAEALPATLRGLANRNALALGDAGWVADLARLEAALVQWVAPPVSPAPV
                     RGRRALAAAIAALVLALGGGWWWLRDGDGGLQAIVGEWSAEVAYPWNLTLREHYVFTL
                     EDGRIGGSASFLGVPRALEAVAWGDGRLRFLTRSETVSGNEPPREMGQLYEVWREGET
                     LRVRLQIRRSHSVDAALEFTAQRQ"
     gene            complement(1600..>2223)
                     /locus_tag="PKV97_16585"
     CDS             complement(1600..>2223)
                     /locus_tag="PKV97_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HNB07552.1"
                     /translation="VPAAAAAPARAEAMAETTDRIIALGTSTGGTQALEAVLRGLPRT
                     VPGIVVVQHMPEKFTAMFAQRLDQVCALEVLEARDGQRVLPGRVLIAPGGRHMTVRRS
                     GAQYLVEVRDGPPVNRHRPSVDVLFRSVARCAGRNALGVIMTGMGDDGARGLREMFDA
                     GAQTIAQDEASSVVFGMPKEAIRLGAATRILALQAIGPAIEQYTRAS"
BASE COUNT          318 a          841 c          802 g          262 t
ORIGIN      
        1 gagcgacgca agtcgcgatc ggctgcggcc ccgcaggcgc cgtgcttgcc gtaggagcga
       61 cgcgagtcgc gatcgcgtgc ggctccggaa gcgcggtgct tgccgtggga gcgacgcgag
      121 tcgcgatcgc gtgcggctcc ggaagcgcgg tgcttgccgt aggagcgacg cgagtcgcga
      181 tcgggccgtg cgccccagtg ctgagccggc ctcattggcg ctgcgcggtg aactccagtg
      241 ctgcatcgac cgaatggctg cggcggatct gcaggcgcac gcgcagcgtc tcgccctcgc
      301 gccagacctc gtagagctgg cccatctcgc gcggcggctc gttgccggac acggtctcgc
      361 tgcgggtgag gaagcgcagc cgcccgtccc cccacgccac cgcctccagc gcgcgcggca
      421 cgccgaggaa gctcgcgctg ccgccgatgc ggccgtcctc cagcgtgaac acgtaatgct
      481 cgcgcagcgt caggttccac ggataggcga cctcggcgct ccattcgccg acgatcgcct
      541 gcagcccccc gtcgccatcc cgcagccacc accagccgcc gccgagtgcg aggaccagcg
      601 ccgcgatcgc ggccgcgagc gcccgccgtc cacgcaccgg tgcgggcgac acgggcggcg
      661 ccacccactg cacaagcgcc gcctccaggc gggcgagatc cgcaacccag ccggcatcgc
      721 cgagcgcgag cgcgttgcgg ttggccagcc cgcgcagggt cgccggcagc gcctcggccg
      781 cgggcatcgt cgccccgccg accagcaccg gaatcaaggg tttccccgag gcgagcgcga
      841 gctcgacctc gcggcgcacg tagtcgccgg gatcgtcgag ccgacgcccg cgcccgtcgc
      901 gcgcgtcgag ccagcgcgta ccgatcacca ccagcaccgc ctgcacctgc tgcagggcct
      961 gctcgatgac cacaccgaaa tcggcccctg cggagatgtc gtcgacatcg cggaacacgc
     1021 tgtcggcgcc gaagcggcgt tcgagcgcgt cggcgagccg cccggcgaag ccggcgctgt
     1081 cgtcgcggcg gtaggagatg aagaccttgg acatggggcg gcaaagcgcg gagagatcct
     1141 gcattcaagc acgccccccg cccccccgcc agcaccgcca ccgaaccaca cgatcgcgac
     1201 tcgcgtcgct cccacagaaa ccagcgtgca cctgcagccg caccgtagga gcgacgcacg
     1261 tcgcgatcgc ggccttcgag ccgccgccgc cgcccaccgc caccgaaccg cccgatcgtg
     1321 actcgcgtcg ctcccacaga aaccagcgtg cacctgcagc cgcaccgtag gagcgacgca
     1381 cgtcgcgatc gcggccttcg agccgccgcc gccgcccacc gccaccgaac cgcccggtcg
     1441 cgactcgcgt cgctcctaca gaaaccagcg tgcacccgca gccgcaccgt aggagcgacg
     1501 caagtcgcga tcgctgcgtg cgggacggcg aggacgcgcg gcgggggggt cgccgtcccg
     1561 cgtcgctcct gcggaaacgt cgcaccgcgg ggcgggggct cagctggcgc gggtgtattg
     1621 ctcgatggcg gggccgatgg cctggagcgc gaggatgcgg gtggccgcac cgaggcggat
     1681 ggcttccttg ggcatgccga agaccacgga gctggcctcg tcctgggcga tggtctgggc
     1741 gccggcgtcg aacatctcgc gcaggccgcg ggcgccgtcg tcgcccatgc cggtcatgat
     1801 cacgccaagc gcgttgcgcc cggcgcagcg ggcgaccgag cggaagagca cgtcgaccga
     1861 ggggcggtgg cggttcaccg gcgggccgtc gcgcacctcg acgaggtatt gcgcgccgct
     1921 gcgccgcacc gtcatgtggc gcccgccggg tgcgatgagc acccgtccgg ggaggacgcg
     1981 ctgtccgtcc cgggcctcga gcacctcgag ggcgcacacc tggtcgaggc gctgggcgaa
     2041 catcgcggtg aacttctccg gcatgtgctg caccacgacg atgccgggta cggtgcgcgg
     2101 caggccgcgc agcaccgcct cgagggcctg ggtgccgccg gtggaggtgc cgagcgcgat
     2161 gatgcggtcg gtggtctcgg ccatggcctc ggcgcgcgcg ggcgccgcgg cagcggcggg
     2221 cac
//
LOCUS       DAOQNN010000497         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_39998_length_2217_cov_8.698890, whole genome shotgun sequence.
ACCESSION   DAOQNN010000497 DAOQNN010000000
VERSION     DAOQNN010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39998_length_2217_cov_8.698890"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PKV97_16590"
     CDS             complement(<1..1019)
                     /locus_tag="PKV97_16590"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB07553.1"
                     /translation="MSFDPSAGHIFAGGAFHHAPGATLQDVVNPADLSVVGRISLCGT
                     AQIDAALEAALVAQRGWAMVDGKSRAAALHRVADAMEKGAQGDVARLMTLEMGKPYSE
                     AVGELLNVGSIFRYYAEIARDDGGFLAGPTAPGSLQYAKYFPYGISAHILPFNFPIVL
                     MAFTVAASLAVGNAVVIKPAPATSLCTLKFMEHFASLPAGLVSCVTGGAETAQALIAD
                     ERVKVVAFTGSVEAGQKVAAACGARLKPCVIEAGGSDPLIVMDSADPDVAAAAAVTAA
                     FHLSGQVCTATERVLVHEKIHDAFVARMVARTKELRVGPGLSNVEIGPLVSQAARDKV
                     MRLVD"
     gene            complement(1016..1360)
                     /locus_tag="PKV97_16595"
     CDS             complement(1016..1360)
                     /locus_tag="PKV97_16595"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB07554.1"
                     /translation="MKTPVLYDPKTVTELKDWGVIPTMIEGASHTSGVLLHKGPNGEN
                     ETGIWVCTPGYWNCHVTRDEFCHFLLGRCTYTHESGEVIEIKPDTIAFFPKDWKGTCR
                     VHETVRKVYMIR"
     gene            complement(1382..1744)
                     /locus_tag="PKV97_16600"
     CDS             complement(1382..1744)
                     /locus_tag="PKV97_16600"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB07555.1"
                     /translation="MTDTLKSSAPHIYNALTFDDLADWGVQPDCTAGPSKSSGRLLFK
                     GPNNEPETGLWVVTPGAWPLSIPRDEFCHFIAGRATYTRDTGEVIEVRPGTCVHFTAG
                     WKGTCVVHETLRNVYMLR"
     gene            complement(1741..>2217)
                     /locus_tag="PKV97_16605"
     CDS             complement(1741..>2217)
                     /locus_tag="PKV97_16605"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB07556.1"
                     /translation="ADVELYQRMIALFARLPYRFLMNVGDYIGEYKDVPGNVHLESWF
                     PQPAVIPHVSLFIHHGGNNSFNEALFFGKPAIIMPFCWDGLDNAARIHDTGYGDQLPR
                     YTWTDEQLSGMIARLIADQGMKARLAEVAKHMQSAKGTVKAANILAEIATKGSYQP"
BASE COUNT          370 a          727 c          747 g          373 t
ORIGIN      
        1 tcgtcgacca ggcgcatgac cttgtcgcgc gcggcctggc tgaccagcgg gccgatctcc
       61 acgttggaaa ggccggggcc gacgcgcagc tccttcgtgc gcgccaccat ccgcgcgacg
      121 aaggcatcgt ggatcttctc atgcaccagc acccgctcgg tcgcggtgca gacctggccc
      181 gagaggtgga aggccgccgt caccgccgcg gcggccgcca cgtccgggtc ggcgctgtcc
      241 atgacgatga ggggatcgct gccgcccgcc tcgatgacgc agggcttcag ccgcgcgccg
      301 caggctgccg ccaccttctg gcccgcctcg acgctgccgg tgaaggcgac caccttcacc
      361 cgctcgtcgg cgatcaaggc ctgcgccgtc tcggcgccgc cggtgacgca ggagacgagg
      421 ccggcaggca ggctggcgaa atgctccatg aacttcagcg tgcagagcga ggtggccggg
      481 gccggcttga tcaccaccgc gttgccgacg gcgagcgagg ccgcgacggt gaaggccatc
      541 agcacgatgg ggaagttgaa gggcaggata tgggcgctga tcccgtaggg gaaatacttg
      601 gcgtattgca gcgagcccgg cgcggtcggc ccggcgagga agccgccatc gtcgcgcgcg
      661 atctccgcat agtagcggaa gatcgatccg acattcagca gctcgcccac tgcttcgctg
      721 tagggcttgc ccatttccag cgtcatcagc cgggcgacat cgccctgcgc gcccttctcc
      781 atcgcatcgg ccacgcgatg gagcgccgcg gcgcggctct tgccgtcgac cattgcccag
      841 ccgcgctggg cgaccagcgc agcctcgagc gccgcgtcga tctgcgccgt accacagagc
      901 gagatgcgcc cgacgaccga gagatcggcg ggattgacca cgtcctgcag ggtggcgccc
      961 ggcgcgtgat ggaatgcgcc gccggcaaag atgtggccgg ccgagggatc gaagctcatc
     1021 ggatcatgta gaccttgcgc accgtctcat gcacccggca ggtccctttc cagtccttcg
     1081 ggaagaaggc gatggtgtcc ggcttgatct cgatcacctc gccggactca tgggtgtagg
     1141 tgcagcggcc gagcaggaag tggcagaact cgtcgcgcgt cacatggcaa ttccagtagc
     1201 cgggcgtgca gacccagatg ccggtctcgt tctcgccatt cggccccttg tgcagcagca
     1261 cgccggaggt gtgcgaggcg ccctcgatca tggtcgggat cacgccccaa tccttgagct
     1321 cggtgacggt cttcgggtcg tagagaacag gcgtcttcat cactcttcac tccaaatgaa
     1381 atcagcgcag catgtagacg ttgcgcaggg tctcgtgcac cacgcaggtg cctttccacc
     1441 cggcagtgaa atgcacgcag gtgccgggcc gcacctcgat cacctcgccg gtgtcgcggg
     1501 tgtaggtggc gcggccggcg atgaaatggc agaactcgtc gcgcgggatg gaaagcggcc
     1561 aggcgcccgg cgtcaccacc cagagccccg tctccggctc gttgttcggc cccttgaaca
     1621 ggagccggcc gctcgacttc gaggggccgg cggtgcaatc cggctgcacg ccccaatccg
     1681 cgaggtcgtc gaaggtgagc gcattgtaga tatgcggcgc cgaggatttc agcgtgtcgg
     1741 tcacggctgg tagctcccct tggtggcgat ctcggccagg atgttcgcgg ccttcaccgt
     1801 gcccttggcg gattgcatgt gtttcgccac ctcggcgaga cgcgccttca tgccctgatc
     1861 ggcgatgagt cgggcgatca tcccgctcag ctgctcgtcg gtccaggtgt agcgcggcag
     1921 ctggtcgcca tagccggtgt cgtggatgcg cgcggcattg tccagcccat cccagcagaa
     1981 cggcatgatg atggcaggct tgccgaagaa caaagcctcg ttgaacgagt tgttgccgcc
     2041 gtgatggatg aacaacgaca cgtgcggaat gaccgccggc tgcgggaacc aggattccag
     2101 gtgcacgttg cccggcacgt ccttgtactc gccgatatag tcgccgacat tcatcaggaa
     2161 gcggtagggc agcctggcga acagcgcgat catgcgctgg tagagctcga catcggc
//
LOCUS       DAOQNN010000498         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_40200_length_2211_cov_6.407236, whole genome shotgun sequence.
ACCESSION   DAOQNN010000498 DAOQNN010000000
VERSION     DAOQNN010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40200_length_2211_cov_6.407236"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2200
                     /locus_tag="PKV97_16610"
     CDS             <1..2200
                     /locus_tag="PKV97_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HNB07557.1"
                     /translation="LGALHARARELLLAARVVPIAKSDGGTRPIAILAVAVRTAIGLQ
                     VGPAARLAAPELREDGQFAIRTASACERVACMVQVAHEAGQAVALVDRRNAYGAVYRR
                     SLRNAVEQRLADCLPLFDTIYGAPTPLCGHVVEGTRASNGVLQGDPLAPLAFALFAAE
                     QAKRLRARQPNVKFVQYLDDIAVIAPDAPTLWAAVDALETDDHIHGLTFVPRKCASNV
                     EPPHQRADWSRFGAAPAKCLGVPVGDTPARREAMGALAHRVAEDLALIGADGLGAYAL
                     LRFCAGAPRMVHALRALDDPIVAEGCVIVDQAVEAALGRVAGVQEVPPHAALPLRMGG
                     CGTVSTRVLAPAARLAGLIATARGDERWPLERPEPNDDAADWCSCGAVKCAAPCNQCA
                     LCCVGDRGEEVCAGCRGALEAAARALPTAQWDWLDEAATTWLATTSVPPPLARGFIAL
                     AGARRQPQRALHQATLQLARRRLLEGASRFLRRRLESNASIPARALYTPDGLVACGLD
                     ATQFAYGLRLRLAYPGATEVSLVSCTCSSAVAGRRAERRRHFVDAPGDINARAHDWHA
                     RACKRGPLARGAHQFVASALSLQFARWGIPARWEAGGIIPEDGGRRCDVLADLPGGAM
                     ALDIARCDVETGALRADSGWPLTAARERLKVRSYTGHLTRLRFAPLVVDEVGRLGPES
                     SKAIRAMAAAIDAMIYAEPGATRAALFACVTAAVIAGQHAQLVLLSCILS"
BASE COUNT          300 a          786 c          795 g          330 t
ORIGIN      
        1 gttgggggcg ctccacgccc gcgcgaggga gctgctgctc gccgcacgtg tggtacctat
       61 tgctaagagc gacgggggca ccaggccgat cgccatcctg gcggtggcgg tccgcaccgc
      121 catcggcctg caggtcggcc cggccgcccg cctcgcagcc ccagagctcc gtgaggatgg
      181 gcagtttgcc atccgcaccg cgagcgcctg cgagcgcgtc gcctgcatgg tgcaggtggc
      241 gcacgaggcg gggcaggccg tggccctcgt cgataggcgc aatgcctacg gcgcggtgta
      301 ccgccgctcg ctgcgcaacg cggtcgaaca gcggctggcc gactgcctgc cgctgttcga
      361 cacgatctac ggcgcgccca cacccctgtg tgggcacgtc gtggaaggca cccgggcttc
      421 caacggagtg ttgcaagggg atccccttgc accactcgcg ttcgcgctct tcgctgcgga
      481 gcaggcgaag cgactgcggg cgaggcagcc caatgtcaag ttcgtgcaat atctcgacga
      541 tattgccgtc atcgcgccgg acgcgcccac gctgtgggcg gcggtggacg cgctcgaaac
      601 cgacgaccat atccatggtc tgacgttcgt gccgcgaaaa tgcgcctcca acgtcgagcc
      661 gcctcaccag cgcgctgact ggtcgcgctt tggcgccgcg cccgcaaagt gcctcggcgt
      721 ccccgtgggg gacacgccag cccggcgaga ggcaatgggg gccctcgcgc atcgggtggc
      781 ggaggatctg gcgctgattg gggcggacgg gttgggggcg tatgccctcc tgcgcttctg
      841 cgccggcgcc ccgcgaatgg tccacgcgct gcgcgccctg gacgacccca tcgtggctga
      901 gggctgcgtg atcgtcgacc aagccgtgga ggcggctctg ggccgcgtcg cgggcgtgca
      961 ggaggtgccc ccccacgccg ccctgccgct gcggatgggc gggtgcggca cggtgtcaac
     1021 gcgggtgctg gcccccgcgg cgcggctggc tggcctcatc gccacagcgc gcggggacga
     1081 gcgctggcca ctggagcggc ccgagcccaa cgacgatgcg gctgattggt gcagttgtgg
     1141 tgcggtgaag tgcgccgccc cctgcaacca atgcgcgctg tgttgcgtcg gcgaccgcgg
     1201 ggaggaagtg tgcgccggct gccgcggcgc gctggaggcg gcagcgcgtg cgctgcccac
     1261 cgcgcaatgg gactggctgg acgaggcagc gaccacatgg ctcgccacca cctctgtccc
     1321 cccgccgttg gcccgcggct tcatcgccct tgctggagcc aggcgccaac cgcagagagc
     1381 cctgcaccag gcgaccctgc agctggctcg caggcgactc ctcgagggag cctcccgctt
     1441 cctcagacgt cgcctcgagt ctaacgcctc catccccgcg cgcgccctgt acacgcccga
     1501 cgggttggtg gcgtgcggcc tcgacgccac tcagttcgcg tacggcctac gcctgcggct
     1561 ggcatacccg ggggcgacgg aggtgtcgct tgtctcgtgc acctgctcat cggctgtagc
     1621 ggggagacgc gcggagcgcc ggcgccactt cgtcgacgcg cccggtgata tcaacgcgcg
     1681 cgcccacgac tggcacgcca gggcgtgcaa acgggggccc ctcgcgcgcg gggcgcacca
     1741 gttcgtcgcg tcggccctga gcctccagtt tgcgcgctgg ggcatccccg ctcggtggga
     1801 ggcgggcggg atcatccccg aagacggcgg acgccggtgc gacgtgctcg cagaccttcc
     1861 cggaggggcg atggcgttgg acatcgcgag atgtgacgtc gagacgggag cgctgagggc
     1921 ggacagcggc tggcccctca ctgcggcacg cgagcgcctg aaggtgcgct catacaccgg
     1981 gcacctcacg cgcctccggt tcgcgcccct ggtggtggac gaggtgggcc ggctcgggcc
     2041 cgagagctcc aaggccatcc gcgccatggc agcagccatc gacgcgatga tctacgccga
     2101 gcccggcgcg acccgtgccg ccctcttcgc ctgcgtcacc gccgcggtga tcgccggcca
     2161 acacgcgcag ctggtgttat tatcatgtat cctatcataa gtactgctac a
//
LOCUS       DAOQNN010000499         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_40557_length_2200_cov_7.886247, whole genome shotgun sequence.
ACCESSION   DAOQNN010000499 DAOQNN010000000
VERSION     DAOQNN010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40557_length_2200_cov_7.886247"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..153
                     /locus_tag="PKV97_16615"
     CDS             7..153
                     /locus_tag="PKV97_16615"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07558.1"
                     /translation="MKGALIALDFMETRHAPPLWRRLLLGWLAGVIGLIVLAWWIARW
                     RAGG"
     gene            complement(185..985)
                     /locus_tag="PKV97_16620"
     CDS             complement(185..985)
                     /locus_tag="PKV97_16620"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNB07559.1"
                     /translation="MLTDSTFREIADLMRATIGLALPPSKKSLVAARLAPRLQRLELA
                     TYEDYLTRLLREHDDGELQVAIDLLTTNETYFFREAPHFALLERDLARGGVAAPAVWS
                     AASSFGDEAYSIAMVLADLESRGHIGRGWSVLGTDISDRVLRAAQAAIFPQERLREVS
                     PERLRRYCLRGDGDFEGLAKMQPALTRRVHFGWLNLFEPIEPLGPFDAIFLRNVLIYF
                     DVPTRRAVVERVLQRLKPGGLFFIGMAEGRIEIGSRLQVLGPGAFRKL"
     gene            complement(1032..1541)
                     /locus_tag="PKV97_16625"
     CDS             complement(1032..1541)
                     /locus_tag="PKV97_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNB07560.1"
                     /translation="MAQAPADKGDRPLQYLTFWLGEEVFGMDIRTVREIIQAGPMTAL
                     PLMPAFVRGVINLRGSVVPVVDLNARFGRTASTVGKKSCVVVFDTLRKGERVELGLLV
                     DAVSEVIKISPADIEPPPDFGSVVRRDFILGIGKVGRRFVVLLEPDRALDVQEMAELC
                     DRAQESALA"
     gene            complement(1584..>2200)
                     /locus_tag="PKV97_16630"
     CDS             complement(1584..>2200)
                     /locus_tag="PKV97_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB07561.1"
                     /translation="KISIVDDIAYQTNLLALNAAIEAARAGEHGRGFAVVADEVRKLA
                     ERSQEAAKEIGDLASSSVSTAERAGRLLDEIVPSIRKTSELVQEIAASSEEQSQAVGQ
                     IGGAMGQLSKATQQNASASEELAATAEELTGQADALQRSISFFAQGETDLSGRNASNG
                     GEPFADRRAPNSPMRKLSRSAVAAAPAPAPKAVANAGRGNFRPY"
BASE COUNT          318 a          733 c          806 g          343 t
ORIGIN      
        1 gccttcgtga agggcgcgct gatcgcgctg gacttcatgg agacgcgcca cgcgccgccg
       61 ctgtggcgca ggctgctgct gggctggctg gccggggtga tcggcctgat cgtgctggcc
      121 tggtggatcg cgcgctggcg cgcagggggc tgaccggggc ctgggcgggg gcgctgcggc
      181 gccgtcagag cttgcggaag gcgccgggtc cgagcacctg caggcgcgag ccgatctcga
      241 tgcggccctc cgccatcccg atgaagaaca gcccaccggg cttgaggcgc tgcagcacgc
      301 gctcgaccac ggcgcgacgc gtgggcacgt cgaagtagat cagcacgttg cgcaggaaga
      361 tcgcgtcgaa ggggccgagc ggttcgatcg gctcgaagag gttgagccag ccgaagtgca
      421 cccgccgggt cagcgccggc tgcatcttgg ccagaccctc gaagtcgccg tcaccgcgca
      481 ggcagtagcg ccgcagccgc tcgggcgaga cctcgcgcag acgttcctgc gggaagatgg
      541 ccgcctgggc ggcacgcagc acgcggtcac tgatgtcggt gccgagcacc gaccagccgc
      601 ggccgatgtg cccgcgcgac tccaggtcgg ccagcaccat cgcgatgctg taggcctcgt
      661 cgccgaagga cgaggccgcg ctccacaccg ccggtgcggc cacgccgccc ctggccagat
      721 cgcgctcgag cagtgcgaag tggggcgcct cccgaaagaa gtaggtctcg ttggtggtca
      781 gcaggtcgat ggccacctgc agttcaccgt cgtcgtgctc gcgcagcagc cgcgtgaggt
      841 aatcctcgta cgtcgccagc tcgaggcgct gcagccgggg cgcaaggcgc gcagccacca
      901 gcgatttctt gctgggcggc agggccaggc cgatcgtcgc acgcatcagg tcggcgatct
      961 cgcggaaggt gctatcggtc aacatgtgtt cgacgcaggc ctgtgggggc cgaagagcgg
     1021 ctgcacttgg gtcaagccag tgcgctttcc tgtgcgcggt cgcagagttc ggccatttcc
     1081 tgcacgtcca gcgcccggtc gggttcgagc agcacgacga agcgccgccc gaccttgccg
     1141 atgccgagga tgaagtcgcg ccggaccacc gagccgaagt ccggtggtgg ctcgatgtcg
     1201 gccggcgaga tcttgatgac ctcgctgacc gcatcgacca gcagaccgag ttcgacgcgt
     1261 tcgcccttgc gcagcgtgtc gaacaccacc acgcagctct tcttgcccac cgtggaggcg
     1321 gtccgtccga agcgggcgtt caggtcgacc accggaacca ccgagccgcg caggttgatg
     1381 acaccgcgca cgaaggcggg catcagcggc agcgcggtca tcgggccggc ctggatgatc
     1441 tcgcgcacgg tgcggatgtc catgccgaac acctcctcgc cgagccagaa ggtgaggtac
     1501 tgcaaggggc ggtcgccctt gtcggcgggt gcctgggcca tgaggggctc ctgatgatcg
     1561 gttcgcgggg gtgaatggcc ggctcagtac gggcggaagt tgccgcgccc ggcgttggcc
     1621 acggccttcg gtgccggagc cggcgccgcg gccacggccg accggctcag cttgcgcatc
     1681 ggcgagttcg gcgcccggcg atcggcgaag ggttcaccgc cattcgaggc gttgcgcccg
     1741 gacaggtccg tctcgccctg agcgaagaag gagatcgagc gctgcagtgc gtcggcctgg
     1801 ccggtgagct cctcggcggt ggcagccagt tcctcggagg ccgaggcgtt ttgctgcgtg
     1861 gccttgctga gctggcccat tgcgccgccg atctgaccga ccgcctggct ctgctcctcg
     1921 gacgaggcgg cgatttcctg caccagttcg gaggtcttgc ggatcgaggg cacgatctcg
     1981 tcgagcagcc ggccggcgcg ctccgcggtg gagacgctgg aggaggccag gtcgccgatc
     2041 tccttggccg cctcctggct gcgttcggcg agcttgcgca cctcgtcggc caccacggcg
     2101 aaaccgcgac cgtgctcgcc cgcgcgtgcc gcctcgatcg cggcattgag cgccagcagg
     2161 ttggtctggt aggcgatgtc gtcgacgatg ctgatcttgg
//
LOCUS       DAOQNN010000500         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_40590_length_2199_cov_5.930504, whole genome shotgun sequence.
ACCESSION   DAOQNN010000500 DAOQNN010000000
VERSION     DAOQNN010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40590_length_2199_cov_5.930504"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            458..1528
                     /gene="glcE"
                     /locus_tag="PKV97_16635"
     CDS             458..1528
                     /gene="glcE"
                     /locus_tag="PKV97_16635"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HNB07562.1"
                     /translation="MTMIEKEWAERVRAAVATRTPLCLRGGGSKDFFGCTLAGERFDL
                     RANRGVVAYEPTELVVTVCAGTPLAELEAVLADKGQYLAFEPPHFGPGATVGGCVAAG
                     LSGPRRASAGAVRDFMLGVKLLDGRGEVLRFGGQVMKNVAGYDVSRLVAGSLGTLGIL
                     LEVSLKVLPRPVAEATLRFELDEASALQRVNEWGGQPLPVSATLWQHGVLHLRLSGAE
                     AAVQAACARLGGEALDDATAQALWTAAREQTLPGFSLEEGEVLWRLSLPSTAPALGLP
                     GRQLVEWGGAQRWFAGAIDAQAVGARAVELGGHATAFRGGDRAAGVFQPLAAPLAVVH
                     RRLKQAFDPAGIFNPDRLYPEL"
     gene            1532..>2199
                     /gene="glcF"
                     /locus_tag="PKV97_16640"
     CDS             1532..>2199
                     /gene="glcF"
                     /locus_tag="PKV97_16640"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HNB07563.1"
                     /translation="MQTQLADFIRDTPEGREAEEILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGAAPTEKTRLHLDRCLTCRSCESTCPSGVHYSRLLDIGRAVV
                     AERVPRHGGEALVRRTLREFVPRPRLFGLAMGAGRAVRGLLPKSLQDKVPTLRPAGTW
                     PAPRHARRVIALAGCAQPAMAPSINAAAARVLDALGISMVELPGAGCCGAVRVLAVQP
                     PAGVV"
BASE COUNT          266 a          777 c          830 g          326 t
ORIGIN      
        1 accgctgcgc cgaatacggc cgcatgaagg tgagcaaggg gcatgtgccg tatcccgaga
       61 ttccgcggtt ctgagcgggg gaatcgcgac ttgcgtcgct cccacgggcg gtcccgggcg
      121 gcggtgaatc gcggatggtc acggggtttc cgtaggagcg acgccagtcg cgatcgccgc
      181 cgacgagcga tggccttccg taggagcgac gcgagtcgcg atcgccgccg acgagcgatg
      241 gccttccgta ggagcgacgc gagtcgcgat cgccgccgac gagcggtggc cttccgtagg
      301 agcgacgcca gtcgcgatcg cggccgacga gcgatggcct tccgtaggag cgacgccagt
      361 cgcgatcgcc gccgacggac tgcgccccgc gcgtcgggag tgcccggccg gcagggtgtg
      421 cggccccgca tatgaatgat gattggacga tgacacgatg acgatgatcg agaaggaatg
      481 ggccgagcgc gtgcgcgcgg ctgtggcgac gcgtacgccg ctgtgcctgc gcggcggcgg
      541 cagcaaggac ttcttcggct gtacgctcgc cggcgagcgc ttcgacctgc gcgctaaccg
      601 cggcgtggtc gcctacgaac ccaccgagct cgtcgtgacc gtgtgtgcgg gcacgccgct
      661 cgccgagctc gaagcggtgc tggcggacaa gggccagtac ctcgctttcg aaccgccgca
      721 tttcggtccc ggcgccacgg tgggcggctg cgtggccgcc ggcctgtcgg ggccgcggcg
      781 ggcgagtgcc ggcgcggtgc gcgacttcat gctcggcgtc aagctgctcg acggccgtgg
      841 cgaggtgctg cgcttcggtg gccaggtcat gaagaacgtc gccggctacg acgtctcccg
      901 cctggtggcg ggcagcctcg gcacgctcgg catcctgctc gaggtctcgc tcaaggtgct
      961 gccgcggccg gtggccgagg ccacgctgcg tttcgagctc gacgaggcca gcgcgctgca
     1021 gcgcgtcaat gaatggggcg gccagccgct gccggtgtcg gccacgctgt ggcagcacgg
     1081 cgtgctgcac ctgcgcctgt cgggcgccga ggcggcggtg caggcggcct gtgcgcggct
     1141 cggcggcgaa gcgctcgatg acgcgacggc gcaggcgctg tggaccgcgg cgcgcgagca
     1201 gacccttccc ggcttctcgc tggaggaggg cgaggtgctg tggcggctgt cgctgccctc
     1261 gaccgcgccc gcgctcggcc tgcccgggcg ccagctcgtc gagtggggcg gcgcgcagcg
     1321 ctggttcgcg ggcgcgatcg acgcgcaggc ggtcggcgcg cgcgccgtcg agctcggcgg
     1381 ccatgccacg gccttccgcg gtggcgatcg cgccgccggc gtgttccagc ccctggcggc
     1441 gccgcttgcg gtggtgcatc gccggctcaa gcaggccttc gaccccgcgg ggatcttcaa
     1501 ccccgaccgt ctctacccgg agctctgagc catgcagacc cagctcgccg atttcatccg
     1561 cgacaccccc gagggccgcg aggccgagga gatcctgcgc aagtgcgtgc attgcggctt
     1621 ctgcaccgcc acctgcccga cctaccagct gctcggcgac gagctcgacg gcccgcgcgg
     1681 gcgcatctac ctgatcaagc aggtgctcga gggtgccgcg cccaccgaga agacccgcct
     1741 gcacctcgac cgctgcctca cctgtcgttc ctgcgaatcc acctgcccct cgggcgtgca
     1801 ctacagccgc ctgctcgaca tcggccgcgc ggtggtggcc gagcgcgtgc cgcgccacgg
     1861 cggcgaggcg ctggtgcggc gtacgctgcg cgagttcgtg ccgcggccca ggctgttcgg
     1921 cctggcgatg ggcgccggac gcgcggtgcg cggcctgctg ccgaagtccc tgcaggacaa
     1981 ggtgcccacg ctgcggccgg ccggcacctg gccggcgcca cgccatgcgc gccgggtgat
     2041 cgcgctcgcc ggttgcgcgc agcccgcgat ggcgccttcg atcaacgccg ccgcggcgcg
     2101 cgtgctcgac gcgcttggca tctcgatggt ggagctgcct ggtgccggct gctgcggtgc
     2161 ggtgcgtgtt ctcgcggttc aaccgcccgc cggggttgt
//
LOCUS       DAOQNN010000501         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_40702_length_2196_cov_7.432975, whole genome shotgun sequence.
ACCESSION   DAOQNN010000501 DAOQNN010000000
VERSION     DAOQNN010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40702_length_2196_cov_7.432975"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PKV97_16645"
     CDS             complement(<1..872)
                     /locus_tag="PKV97_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNB07564.1"
                     /translation="MGTRIGIDLGGTKIELVVLDADGRERWRRRVPTPQGDYGGMLRA
                     IAALVEEAERLTGAGARIGVGTPGSPSPRDGRIRNANSTCLNGQPLQQDLEALLRRPL
                     RLANDANCLAMSEAADGAGAGARTVFAAILGTGVGGGIVVDGKLLVGANAVAGEWGHN
                     PLPLPAPDDLPLPACYCGRAGCIETYLSGPGLAADHLRHGGEPLDAAAIARQATEGQR
                     ASQASLERYELRLARALAGVINLLDPEVIVLGGGLSQIARLYEHVPRRWAAHVFSDTV
                     STRLLPARHGDASGV"
     gene            complement(889..1536)
                     /gene="can"
                     /locus_tag="PKV97_16650"
     CDS             complement(889..1536)
                     /gene="can"
                     /locus_tag="PKV97_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNB07565.1"
                     /translation="MSQSIEHLFANNKNWSERMHSEDPEFFSRLVKQQSPEYLWIGCS
                     DSRVPANQVIGLAPGEVFVHRNIANVVVHTDLNALSVIHYAVEILRVKHILVVGHYGC
                     GGVKAAMNDNKTGLTDNWLRHIQDVRDRHIDRLERFTDPAERADRLCELNAMHQVVNV
                     CQTSVLREAWQRGQGVTVHGWCYSLQDGLVRDLGVSAGTREEAVERYRDAVERIG"
     gene            1735..>2196
                     /locus_tag="PKV97_16655"
     CDS             1735..>2196
                     /locus_tag="PKV97_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNB07566.1"
                     /translation="MNLNPQQARSLVAICLHAAFADGHQGAVERDHVRKVAESLGREA
                     EIDFIALYQDVLLGGLGLEAAVAGLGEANLRQLAFELAVGVCEADGGLDAREKDFLER
                     LSGLLELSRPAADAFVREAEALGQAPADGPLASLPGLGEDEPAAAPSAAAAP"
BASE COUNT          295 a          751 c          820 g          330 t
ORIGIN      
        1 acgcccgagg cgtcgccgtg ccgtgccggc agcaggcgcg tggacacggt gtcggaaaac
       61 acgtgcgcgg cccaccgccg cggtacgtgc tcgtacaggc gcgcgatctg cgacaggccg
      121 ccgccgagca cgatgacctc ggggtcgagc aggttgatca cgcccgcgag tgcgcgcgcg
      181 agccgcagct cgtagcgttc gagactggcc tgagaggcgc gctggccctc ggtggcctgg
      241 cgggcgatgg ctgcagcgtc gaggggctcg cccccatggc gcagatgatc ggcggccagg
      301 ccgggaccgg acaggtaggt ctcgatacag ccggcgcgtc cgcaatagca ggcgggcagg
      361 gggaggtcgt cgggcgcggg cagcggcagc gggttgtggc cccattcgcc ggccaccgca
      421 ttggcgccca ccagcagctt gccgtcgacg acgatgccgc cgccaacccc ggtgccgagg
      481 atggccgcga acacggtccg cgcacccgcg ccggcgccgt cggcggcctc cgacatggcc
      541 aggcagttgg catcgttggc gaggcggagc ggtcggcgca gcagggcttc gaggtcctgc
      601 tgcagcggct ggccgttcag gcaggtggag ttggcgttgc ggatgcggcc gtcgcgcggc
      661 gaaggcgacc ccggtgtgcc gacgccgatg cgggcgcccg cgccggtgag gcgctcggcc
      721 tcctcgacca gcgcggcgat ggctcgcagc atgccgccgt agtcgccctg cggggtcggc
      781 acccggcggc gccagcgctc gcgaccgtcg gcgtcgagca ccacgagctc gatcttggtg
      841 ccgccgaggt cgatgccgat gcgggtcccc atgccgtggc gggcagcgtc agccgatgcg
      901 ctcgaccgcg tcgcgataac gctccacggc ctcctcgcgg gtgccggcgc tgacgccgag
      961 gtcgcgcacg aggccgtcct gcaggctgta gcaccagccg tgtacggtga cgccctggcc
     1021 gcgctgccag gcctcgcgca gcaccgaggt ctggcacacg ttcaccacct ggtgcatggc
     1081 gttgagctcg cacaggcggt cggcgcgctc ggccgggtcg gtgaagcgct ccaggcgatc
     1141 gatgtggcgg tcgcgcacgt cctggatgtg gcgcagccag ttgtcggtga ggccggtctt
     1201 gttgtcgttc atcgccgcct tcacgccgcc gcagccgtag tggcccacga cgaggatgtg
     1261 cttcacgcgc aggatctcga ccgcgtagtg gatgaccgag agtgcgttga gatcggtatg
     1321 cacgaccacg ttggcgatgt tgcgatgcac gaagacctcg cccggcgcga gcccgatcac
     1381 ctggttcgcc ggcacccggc tgtcggagca gccgatccac aggtattccg gcgactgctg
     1441 cttgaccagg cgcgagaaga actcggggtc ctcggagtgc atgcgttccg accagttctt
     1501 gttgttggcg aagagatgtt cgatcgactg ggacatggtg tgtgcgggcc tggagtgcgg
     1561 taacgcgtaa ttatgtccgc gccggccgac ggcggccagc atcggcgcgc cgtgctcgca
     1621 gcacgggggg atcgtcccgc cgccgcgccg gtcgaatcgt tacagcgtgt catgcgcgct
     1681 tgaagcgcca ccgcccatcc ccaacctgaa tgttttccac cgcggagtcc gtccatgaac
     1741 ctgaatccac agcaagcccg ttccctcgtc gcgatctgcc tgcatgcggc cttcgccgac
     1801 ggtcaccagg gggcggtaga gcgcgaccat gtgcgcaagg tcgccgaatc gctcgggcgc
     1861 gaggcggaga tcgacttcat cgcgctctac caggacgtcc tgctcggcgg gctcggcctg
     1921 gaggcggcgg tggccgggct gggcgaggcg aacctgcgcc agctcgcctt cgagctggcg
     1981 gtcggggtgt gcgaggccga tggtgggctg gatgcgcgcg agaaggactt cctcgagcgc
     2041 ctgtccggtt tgctcgagct ctcgcgaccc gctgcggacg ccttcgtgcg cgaggcggaa
     2101 gcgctcggcc aggctccggc cgacgggccg ctcgccagcc tgcccggcct gggcgaggac
     2161 gagccggcgg ctgcgccgtc cgccgccgcc gccccc
//
LOCUS       DAOQNN010000502         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_41119_length_2184_cov_10.945045, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000502 DAOQNN010000000
VERSION     DAOQNN010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41119_length_2184_cov_10.945045"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PKV97_16660"
     CDS             complement(<1..866)
                     /locus_tag="PKV97_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNB07567.1"
                     /translation="MALGVPPLQAASSDARWKGAAFEGDRASLARLAAAGAKVVRVYR
                     QSDAWVLDEAQRLGLKVVMGLWLEHPRHGFDYADARAVRAQEDAVLDFVARYRHHPAL
                     LAWGVGNEIETGVADPLPLWRAVDRLAARIRALDPEHPTMMVVADTGMDAFRTLAGCC
                     PNVELLGINVYAGAVFDLPQRLRDAGIAKPVVVAELGPLGQWQAGRKPWGAPVELTST
                     EKARFFTEALAFLDQQAQIRGVFPFLWGAKQEQTATWHGLLLADGSPTAMSDALAAAW
                     GRSCDAVNPPARP"
     gene            1072..1362
                     /gene="groES"
                     /locus_tag="PKV97_16665"
     CDS             1072..1362
                     /gene="groES"
                     /locus_tag="PKV97_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNB07568.1"
                     /translation="MKIRPLHDRVIVKRLEAERKTASGIVIPDSAGEKPDQGEVLAVG
                     NGKILDDGKVRPMAVKVGDKVLFGKYAGQTVKVEGDELLVMREEDIMGVVEA"
     gene            1415..>2184
                     /gene="groEL"
                     /locus_tag="PKV97_16670"
     CDS             1415..>2184
                     /gene="groEL"
                     /locus_tag="PKV97_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNB07569.1"
                     /translation="MAAKEVKFGDSARERMVAGINILANAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDIAGDGTTTATVLAQSIVRE
                     GMKFVAAGMNPMDLKRGIDKAVVATIDELKKLSKPCSTNKEIAQVGSISANSDSDIGD
                     IIARAMDKVGKEGVITVEDGKSLQNELDVVEGMQFDRGYLSPYFINNPDKQVAILEQP
                     FVLLFDKKISNIRDLLPVLEQVAKSGRPLLIIAEDVEG"
BASE COUNT          415 a          724 c          723 g          322 t
ORIGIN      
        1 gggcgcgccg gagggttgac cgcgtcgcaa gagcgccccc aggcggcggc aagcgcgtcg
       61 ctcatcgcgg tggggctgcc gtcggcgagc agcagtccgt gccaggtcgc ggtctgttcc
      121 tgcttcgcgc cccacaggaa ggggaagacg ccgcggatct gcgcctgctg gtcgaggaag
      181 gcgagcgcct cggtgaagaa gcgcgccttc tcggtgctgg tgagctcgac cggcgcgccc
      241 cagggcttgc gtccggcctg ccactgcccg agcgggccga gctcggcgac caccaccggc
      301 ttggcgatgc cggcgtcgcg caggcgctgc ggcaggtcga acaccgcgcc ggcatagacg
      361 ttgatgccga gcagctcgac gttggggcag cagccggcga gcgtgcggaa ggcatccatg
      421 ccggtgtcgg caaccaccat catcgtcgga tgctcggggt cgagcgcgcg gatgcgcgcg
      481 gccagccgat ccaccgcgcg ccacagcggc agcgggtcgg cgacgccggt ctcgatctcg
      541 ttgcccacgc cccaggcgag cagggcaggg tggtggcggt agcgggcgac gaagtcgagc
      601 acggcgtctt cctgtgcgcg cacggcgcgc gcgtcggcgt agtcgaaacc gtggcgcggg
      661 tgctccagcc acagccccat caccaccttc aggccgaggc gctgcgcctc gtcgagcacc
      721 caggcatcgg actggcggta cacgcgcacg accttggcgc cggcggcggc gaggcgggcg
      781 aggctggcgc ggtcgccctc gaaggcggcg cccttccatc gtgcgtccga ggaggcagcc
      841 tggagggggg gcacgcccag cgccatcacg gcgaccaaaa aagttaacag atagcgcact
      901 tgaaatcctc gggacccgtc cctattatta gcactcgttg gcggtgagtg ctaacaagcc
      961 tcagtcgatg gtcttccgcc catcggcagc cactgcagat ccttttcttg cggactggtt
     1021 ccgcatgttt catcaaccca gacagttgaa cctcagttag gagagaactc gatgaagatt
     1081 cgtcccctgc atgatcgcgt gatcgtcaag cgcctcgaag ccgaacgcaa gaccgccagc
     1141 ggcatcgtga tcccggacag cgccggcgag aagcccgatc agggcgaagt cctcgcggtc
     1201 ggcaacggca agatcctcga cgacggcaag gtccgtccga tggccgtcaa ggtcggtgac
     1261 aaggtgctgt tcggcaagta cgccggccag accgtgaagg tcgaaggcga cgaactcctc
     1321 gtgatgcgcg aggaagacat catgggcgtg gtcgaggcct gagcctcccc cgcaccgatc
     1381 aaccctaatc gaaacgattc cggagttctg aagaatggca gctaaagaag tcaagtttgg
     1441 tgattccgcc cgcgagcgca tggtcgccgg catcaacatc ctcgccaacg cggtcaaggt
     1501 gaccctgggc ccgaagggcc gcaacgtcgt gctcgagcgc tcgttcggcg cccccaccgt
     1561 gaccaaggac ggcgtctccg tcgccaagga aatcgagctg aaggacaagt tcgagaacat
     1621 gggcgcgcag atggtcaagg aagtcgcttc caagacctcg gacatcgccg gtgacggcac
     1681 caccaccgcg accgtgctgg cgcagtcgat cgtgcgcgaa ggcatgaagt tcgtcgccgc
     1741 cggcatgaac ccgatggacc tcaagcgcgg catcgacaag gccgtcgtcg ccaccatcga
     1801 cgagctcaag aagctgtcca agccctgctc gaccaacaag gagatcgccc aggtcggctc
     1861 gatctcggcc aactccgaca gcgacatcgg cgacatcatc gcccgcgcca tggacaaggt
     1921 cggcaaggaa ggcgtgatca ccgtcgaaga cggcaagtcg ctgcagaacg aactcgacgt
     1981 ggtcgagggc atgcagttcg accgcggcta cctgtcgccc tacttcatca acaacccgga
     2041 caagcaggtc gccatcctcg agcagccctt cgtcctgctc ttcgacaaga agatctccaa
     2101 catccgcgac ctcctgccgg tgctcgagca ggtcgccaag tcgggccgtc cgctgctgat
     2161 catcgccgag gacgtcgaag gcga
//
LOCUS       DAOQNN010000503         2173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_41466_length_2173_cov_6.935788, whole genome shotgun sequence.
ACCESSION   DAOQNN010000503 DAOQNN010000000
VERSION     DAOQNN010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41466_length_2173_cov_6.935788"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PKV97_16675"
     CDS             complement(<1..1318)
                     /locus_tag="PKV97_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02099 family protein"
                     /protein_id="HNB07570.1"
                     /translation="MRRQRCNRAPTQRPASIRLSPSAPHAPAESVPPSVDGLPAARGG
                     WRRAAGRLSDALLLVWCVLAVGVLVLRHLVLPAVGELRVPIAELLGERLGVAVAIERI
                     EGGWAGWRPRLRLAGVSLSDAQGRPALTLPEIDATLAWSSLAMLEPHFHRLEIRAPDL
                     LVRRDPEGALHVAGIALAHGDEGGDGGLGWLLAQRQILIHGARLAWADEARGAPVLEL
                     EEVEFRLDRRTRPARFALEARLPAALGERASLRGELRRIDPLRPQRFAGRLYVELGRT
                     ALGNWRPWVDLPFGLEGEGALQAWLDADGRGGHALTADLALDGVRAVLGEDLPVLELA
                     RLDGRVELARGEDGFRFAGRGLGFETAQGLVLPPTDVELGLDPALDGKARDRGTGEEQ
                     AGRGPAAAGGRLVADRLELATLVALAAHLPLDAGLRSRLAALAPRGR"
     gene            1437..>2173
                     /locus_tag="PKV97_16680"
     CDS             1437..>2173
                     /locus_tag="PKV97_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HNB07571.1"
                     /translation="MSAPNSVSAPALPPAVAQARRLSRYLARMLDSQPWLAEALAASI
                     ERPLAEADLQRFVAAREVLHGGGDGALRPALRQLRMWTLCHLIVRDLALGAPLAEVTE
                     TMTLLAEVALRHAHDVLRAGLVERHGQPLSPTGWEQELLVVGMGKLGGRELNVSSDID
                     LIFIYPEDGDTGGRKAISNYEFFEKLGKQLIQALAEITEHGQVFRVDMRLRPNGDSGP
                     LVCCFDMLENYFVTQGREWERYAWIKAR"
BASE COUNT          309 a          835 c          771 g          258 t
ORIGIN      
        1 cccgaccgcg cggcgcaagc gccgccaggc gcgagcgcag ccctgcatcg agcggcaggt
       61 gggcggcgag cgcgacgagc gtggccagtt cgagccggtc tgcgacgagg cgccccccgg
      121 ccgccgcggg cccgcgcccg gcctgctcct cgccggtgcc acgatcgcgc gccttgccat
      181 cgagcgccgg atccagcccc agctcgacat cggtgggggg cagcacgagg ccctgggcgg
      241 tttcgaaccc gagcccgcgg ccggcgaagc ggaatccatc ctcgccgcgg gccagctcga
      301 cacgaccgtc gagacgggcg agctcgagaa ccggcagatc ctcgccgagc acggcccgca
      361 ccccgtccag cgcgaggtcg gcggtcagcg catgaccgcc acggccgtcc gcgtcgagcc
      421 aggcttgcag cgcaccctcg ccttccaggc cgaaaggcag gtcgacccag ggccgccagt
      481 tgcccagcgc cgtgcgcccg agttcgacgt acaaccgacc ggcgaaacgc tgcgggcgca
      541 gcgggtcgat gcggcgcagc tcgccgcgca ggctcgcacg ctcgccgagg gcggccggca
      601 gccgtgcctc gagcgcgaag cgcgccggac gcgtgcgtcg atcgaggcgg aattccacct
      661 cttcgagctc gagcaccggc gcaccgcgcg cttcgtccgc ccaggcaaga cgcgcgccgt
      721 ggatgaggat ctggcgctgc gcgagcagcc agccgaggcc gccgtcgccg ccctcgtccc
      781 cgtgtgcaag cgcgatgcct gcgacgtgca gcgctccctc cggatcgcgg cgtaccaaca
      841 ggtccggcgc gcggatctcg aggcggtgga aatgcggctc gagcatcgcc agcgaggacc
      901 acgccagggt ggcgtcgatc tcgggcaggg tcagcgcggg gcggccctgc gcgtcggaca
      961 ggctcacccc ggccaggcgc aggcgcggtc gccagcccgc ccagccgccc tcgatgcgct
     1021 cgatcgccac cgccacgcca aggcgctcgc cgagcaactc ggcgatcggc acgcgcagtt
     1081 cgcccactgc cggcagcacg agatggcgca gcaccagcac cccgaccgcg agcacgcacc
     1141 agaccagcag cagcgcatcg gacagcctgc cggccgcgcg ccgccagccg ccgcgcgcgg
     1201 ccggcaggcc gtcgacggac gggggtacgg attcggcagg tgcgtgcggg gcggaggggc
     1261 tcagtcggat gctcgcagga cgttgtgtgg gagcgcggtt gcagcgctgc cggcgcatgg
     1321 cgcgggcagc tagaatattg ggatcgcgcc cgccaccgga tcgccgacgc gcgcgcattc
     1381 taccaagtct cgatcgccct ctcgatcacc ctctcgcgcc gcactggagc cccctcatgt
     1441 ccgccccgaa cagcgtttcc gcccctgccc tcccgcccgc ggtggcgcag gcgcgccgcc
     1501 tgtcgcgcta tctggcgcgc atgctcgaca gccagccctg gctggcggag gcgctggccg
     1561 ccagcatcga gcgcccgctc gccgaggccg acctgcagcg cttcgtggcc gcgcgcgagg
     1621 tcctccacgg tggcggcgac ggcgcgctgc gcccggcgct gcgccagctg cgcatgtgga
     1681 cgctgtgcca cctgatcgtg cgcgacctcg cgctcggcgc cccgctcgcc gaggtcaccg
     1741 agaccatgac cctgctcgcc gaggtggcgc tgcgccacgc ccacgacgtg ctgcgcgcgg
     1801 gattggtcga gcgccacggg cagccgctct cgcccaccgg ctgggagcag gagctcctcg
     1861 tggtcggcat gggcaagctc ggcgggcgcg agctcaacgt ctcctccgac atcgacctga
     1921 tcttcatcta cccggaggac ggcgacaccg gcgggcgcaa ggcgatcagc aactacgagt
     1981 tcttcgagaa gctcggcaag cagctgatcc aggcgctcgc cgagatcacc gagcacggcc
     2041 aggtcttccg cgtggacatg cgcctgcgcc cgaacggcga ctccggcccg ctggtgtgct
     2101 gcttcgacat gctggagaac tacttcgtca cccagggccg cgagtgggag cgctacgcct
     2161 ggatcaaggc gcg
//
LOCUS       DAOQNN010000504         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_41607_length_2169_cov_6.967360, whole genome shotgun sequence.
ACCESSION   DAOQNN010000504 DAOQNN010000000
VERSION     DAOQNN010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41607_length_2169_cov_6.967360"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            617..1498
                     /locus_tag="PKV97_16685"
     CDS             617..1498
                     /locus_tag="PKV97_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB07572.1"
                     /translation="MALCRIPSIQCLLTFEAVARLRSASRAAEELCVTVSAVSHRLRQ
                     LESHLGVRLFSRSDFTPTAEGAAYLQQVGVALAALRELPGERAPGASARLRVAVTPSF
                     CRQFVMPKLELFRRDWPDIELTLQVSIPLRDVVAEEAELEIRYGAGGYTDLEHRLLLS
                     DEAVPACSPAFLDEHGPFDGFASLAECAGTALVRSPLEPWSNWFAHCGLALPEPARGA
                     QFNDLGLAYDAAASGFGVALVRQRLGAAWFDSGRLLALSSRPLRSPYAHYLCWRPGAL
                     ERWECAAFADWLQAALP"
     gene            1890..>2169
                     /locus_tag="PKV97_16690"
     CDS             1890..>2169
                     /locus_tag="PKV97_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNB07573.1"
                     /translation="MMLENEALPFGETEGDFSAVDKARVVAALSKVLPSGTLMYDSED
                     LRPYECDGLAAYRALPLVVALPTTEAQVVAVLRICHEMGVPVVARGAGT"
BASE COUNT          332 a          707 c          746 g          384 t
ORIGIN      
        1 ccaccgccag cgaatagctg ttgccatcgc cgtacgtgac gtaaccgagc ccgttcagat
       61 tgaccaaggc tgccgatgcg gacgatgcgc cgagcgcggc gagcattgcg acgaccaggt
      121 ttctggcttt catggcactc cctcctacag gtgtatggag atctcttggc aaatcgtgtg
      181 ccaataatgc aagtagctgt tttttatata ttttaatttt tcgatttttc ttcttcagca
      241 gcgtgtgtaa aaaaattcga cacccggatc cagccttcgt gcggagatcc gaacgggttc
      301 caagcgaagg gatatgcgaa aggcaattcc tttgcgacgc cttttccgat ggccatggaa
      361 agtaactcgg agttgccgat gtactatgaa aaattaattc gataaatgcc tgaagaataa
      421 ttgcggcacg aatgaagtgg ccgggccgat ccggcgcttc gtcctgcgac cgggagaatg
      481 acgctctcgc gccacagcag gatgcgcgca cccgaaactc ggcgcgggag ggaaggtgcg
      541 ggcgtcgcag tgcaggcagc ggggcagcgg gggcataatc ggggacatcc ttccgaacgg
      601 gccccgagcc gcgaccatgg ccctctgccg catcccgtcc atccagtgcc tgctcacctt
      661 cgaggcggtc gcgcgcctgc gcagcgcgag ccgtgcggcc gaggagctct gcgtgaccgt
      721 cagcgcggtc agccatcgcc tgcgccagct cgagtcccat ctgggcgtgc ggctcttctc
      781 gcgcagcgac ttcaccccga ccgccgaggg ggcggcctac ctgcagcagg tcggtgtcgc
      841 gctcgcggcg ctgcgcgaat tgccgggcga gcgcgcgccg ggtgcatcgg cgcgcttgcg
      901 cgtggcggtg acgccctcgt tctgccggca gttcgtcatg cccaagctcg agctcttccg
      961 ccgcgactgg ccggacatcg agctcaccct gcaggtgtcg atcccgctgc gcgacgtcgt
     1021 cgcggaagag gcggagctcg agatccgcta tggcgccggg ggctacaccg acctcgagca
     1081 ccgcctgctg ctgagcgacg aggcggtccc cgcctgcagc ccggccttcc tcgacgagca
     1141 cggccccttc gacggtttcg cgagcctcgc cgagtgcgcc ggcaccgcgc tcgtgcgcag
     1201 cccgctcgag ccctggtcga actggttcgc gcactgcggc ctggccctgc ccgagccggc
     1261 gcgcggcgca cagttcaacg acctcggcct ggcctacgat gccgcggcga gcggcttcgg
     1321 ggtcgccctg gtgcgccagc gcctgggggc ggcgtggttc gacagcgggc gcctgctggc
     1381 gttgtcctcg cgcccgctgc gctcgcccta cgcgcactac ctgtgctggc gccccggcgc
     1441 gctcgagcgc tgggagtgcg ccgcgttcgc ggactggctg caggccgcgc tgccctgaag
     1501 catcgccggg ccgtttcagg gttgaatttt cttcaagccg gcgagcaaga ggcgtcaagc
     1561 gggcgctgtg cgcgagcctc tatactccgg atagctgccc ggtcccgcgg ggagtcgagg
     1621 attcggatgc gccgaagatc gcgacttgcg tcgctcctac aaggctgcgc acgtcccgag
     1681 cccctgtagg agcgacgcaa gtcgcgatcc atacgatccc gatacgcccg cggggattcc
     1741 gggggcggag gattgcgctt cgcggcggtt ctgttttcgt aggagcgacg caagtcgcga
     1801 ttgccgcggt cccgatacgc ccgcgcggat ttcgatgtgc cggcgcggcg gtcgatgctc
     1861 gatcggcatg gccaggcgag gagacgagga tgatgctgga gaacgaagcg ctgccgttcg
     1921 gtgagaccga gggcgatttt tccgcggtgg acaaggcgcg cgtggtcgca gccttgagca
     1981 aggtgctgcc gtccgggacg ctgatgtacg acagcgagga cctgcgcccc tacgagtgcg
     2041 acggcctcgc cgcctaccgc gcgctgccgc tggtcgtggc cctgcccacc accgaggcgc
     2101 aggtggtggc ggtgctgcgc atctgccatg agatgggcgt gccggtggtg gcgcgtggcg
     2161 cgggcaccg
//
LOCUS       DAOQNN010000505         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_41642_length_2168_cov_7.389967, whole genome shotgun sequence.
ACCESSION   DAOQNN010000505 DAOQNN010000000
VERSION     DAOQNN010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41642_length_2168_cov_7.389967"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..845
                     /locus_tag="PKV97_16695"
     CDS             111..845
                     /locus_tag="PKV97_16695"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNB07574.1"
                     /translation="MRPREQAESLCARLEAAGGRALRFPVIAVGPALDPAPLQAIVGR
                     LDEFDLAFFVSPNAVDHAMRAILPQRTWPARLRVATVGKGSQRVMQGWGFANVIAPQD
                     GFDSEAVIALPEFAADAVRGRKVLIFRGDGGRELLADTLRERGASVEYVTCYRRHCPE
                     ADPALLLGPAARDELDGLLLTSSEGVRNLEWMLGTEGLAALRAVTVFATHPRIVAQAR
                     AAGFERVVETPAGDDGLMQALESHFA"
     gene            856..>2168
                     /locus_tag="PKV97_16700"
     CDS             856..>2168
                     /locus_tag="PKV97_16700"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNB07575.1"
                     /translation="MRQENPALTQPPPATSASPAPEETRPVIEEVGVPEAPPAAEVDP
                     AARGAASEGDPVAPAARTANAAEEQPARSPAAWWALLLALIAVGVAGWSIWQAREMRL
                     QTGQLRAEVASRLSDGETIATEARGMMRQQQEVIASLQGKLGALESRVETTQGQAEAL
                     EALYQEFSRSREDGVLAEVEQAVALASQQLQIAGNVEAALIGLQEAEARLAIHDRGQL
                     ATLRRALVRDIEELRLLPVLDVSGLSLRLELMLERADTLPLAFESPLPAAAAVGAEMG
                     PADGGGFVGWMAGVWRFAQNVAADAWSEIRTLIRVERLDQEDPVLLAPEQNTFLRENL
                     KMRLLTARLALMARDGRSYAADLAQARQWLERFYDLRDERVQAALGELKQLEAVKVRY
                     APPDLSETFSALRSVQSRAGRSGADARGAAAPAPASAPAEAPVATA"
BASE COUNT          292 a          781 c          793 g          302 t
ORIGIN      
        1 ctcgccgagc tcggctgagc ggacggcaag ggaggcgcgc ggtgacgacc ccggcggcag
       61 cttccgatct ccggtccgtg cgtcccctcg ccgggcgcac cctcgtcgtc atgcgcccgc
      121 gcgagcaggc cgaaagcctg tgcgcccgcc ttgaggccgc cggcgggcgt gcgctgcgct
      181 ttccggtgat cgcggtcggt ccggcgctcg atccggcgcc gctgcaggcc atcgtcggcc
      241 gcctcgacga gttcgacctc gccttcttcg tcagccccaa cgcggtcgac cacgccatgc
      301 gcgcgatcct gccgcagcgc acctggcccg cccgcctgcg tgtcgccacc gtgggcaagg
      361 gcagccagcg cgtgatgcag ggctggggct tcgcgaacgt catcgcgccg caggacggtt
      421 tcgacagcga ggccgtgatc gccctgcccg aattcgccgc cgacgcggtg cgcgggcgca
      481 aggtgctgat cttccgtggc gacggcggcc gcgaactgct cgccgacacc ttgcgcgaac
      541 gcggcgcgag cgtggaatat gtcacctgct accgacgcca ttgtcccgag gccgatcccg
      601 cgctgctgct cgggccagcg gcgcgggacg agctcgacgg cctgctgctg accagcagcg
      661 agggcgtacg caatctggaa tggatgctcg gcacggaggg gctcgccgcc ctgcgcgccg
      721 tgacggtgtt cgccacgcac ccgcgcatcg tcgcgcaggc gcgtgcggcc ggtttcgaac
      781 gggtggtcga gacgccggcc ggagacgacg gtttgatgca ggccctagaa agccattttg
      841 cctagacgtg gaaccatgag acaagaaaat cccgctctga cccagccgcc gccggcgacg
      901 tccgcatcgc cggccccgga ggagacccga cccgtcatcg aggaggtggg cgtgcccgag
      961 gcaccgcccg cagccgaggt ggatcccgcg gcgcgcggcg ctgcgagcga gggcgatccg
     1021 gtcgcccccg ccgcacgaac ggcgaacgcc gccgaggagc agcccgctcg cagtcccgcc
     1081 gcgtggtggg cgctgctgct ggcgctgatc gccgtcggcg tggccggctg gtcgatctgg
     1141 caggctcgcg agatgcgcct gcagacggga cagctgcgcg cggaggtcgc cagccgtctc
     1201 tccgatggcg agaccatcgc caccgaggcg cgcgggatga tgcgccagca gcaggaggtc
     1261 atcgcctcgc tgcagggcaa gctcggtgcg ctcgaatcca gggtcgagac cacacagggc
     1321 caggccgagg cgctcgaggc gctgtaccag gagttctcgc gcagccgcga ggacggcgtg
     1381 ctcgccgaag tcgagcaggc cgtcgcgctg gcctctcagc agctgcagat tgcgggcaac
     1441 gtcgaggccg cgctgatcgg cctgcaggag gccgaggccc ggttggcgat ccacgatcgc
     1501 ggccagctcg ccaccctgcg ccgtgcgctc gtgcgcgaca tcgaggagct gcgcctgctg
     1561 ccggtgctgg atgtttccgg cctcagcctg cgcctggagc tgatgctcga gcgtgcggac
     1621 acgctgccgc tcgccttcga gagcccgctg ccggccgccg ctgcggtggg cgcggagatg
     1681 gggcctgccg atggcggtgg cttcgtgggc tggatggccg gtgtgtggcg cttcgcgcag
     1741 aacgtcgcgg ccgatgcctg gagcgagatc cgcaccctga tccgcgtcga gcgcctagac
     1801 caggaagatc cggtgctgct cgcgcccgag cagaacacct tcctgcgcga gaacctcaag
     1861 atgcgcctgc tcaccgcgcg cctcgcgctg atggcgcgcg acggccgcag ctacgccgcc
     1921 gatcttgccc aggcccgcca gtggttggag cgtttctacg acctgcgcga cgagcgggtg
     1981 caggccgcgc tcggcgagtt gaagcaactc gaggcggtga aggtgcgcta cgcgccgccc
     2041 gatctgagcg agaccttcag cgccttgcgc agcgtgcagt cgcgcgctgg ccgcagcggc
     2101 gcggatgcgc gcggcgctgc cgcgcccgct cctgcgtccg cccccgccga agcgcccgtg
     2161 gcgactgc
//
LOCUS       DAOQNN010000506         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_42016_length_2157_cov_15.386775, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000506 DAOQNN010000000
VERSION     DAOQNN010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42016_length_2157_cov_15.386775"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PKV97_16705"
     CDS             <1..257
                     /locus_tag="PKV97_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07576.1"
                     /translation="PATAELMPMTAAIEAASPSLRRCAGLAGETLVVNFSAARGRDRF
                     EAVTVLGGHDDEVERCVREAGAAIRFAPATSQSMMKEYVP"
     gene            254..1255
                     /locus_tag="PKV97_16710"
     CDS             254..1255
                     /locus_tag="PKV97_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07577.1"
                     /translation="MKTLACLLAVSLAGESPAPTQVAGETECVATDNRCQAKRFEQKA
                     SRAETPKMRALYLHAAHVAHLAVFDETGDVGALCAARRTFDKSLAIEGQPEGQRTAFE
                     DARGKLESREKQRGARCGSARRSAKTQARALAAKVEAAAGAGEAEPPPLLAMGPAAGA
                     QVEDSVSASPSEAVTSAAAVAPSEDTLLPVRSSAAASSASSTTPAVDPRSVGASRAPG
                     RPLLFAGSVTLAIGLSLGGVAAYTGRRALVAYQAGLDLHEDVQGPPDAATLARDAELE
                     AEYRRMGPVAIGTAIGGGVTVVVGAVMAGVGGRRLARAGSQTAWAPVPGGLAFHVRF"
     gene            1275..>2157
                     /locus_tag="PKV97_16715"
     CDS             1275..>2157
                     /locus_tag="PKV97_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07578.1"
                     /translation="MATPNRTQLSLVLVTLLSACAPWPEWPAAECSDTSCASSTDTSP
                     GSGGSFGEVTGTVTGVTSGASDDGTAAGSDSATVGETGEEQTTGATTGDPIEPPAIVE
                     VELTPDPIIFNGPIAVTVNAEHAEGVRMKLEDGEEVELVEAEPGVFVGEIAVMTGLLN
                     GDRVVVVTPWAGELMGEAVEASYTIALPAPGSEAFWEAGDLIGGGQVVSMGVLPDGNI
                     VEFGTQWIQGGSRCYVRRRDKSGAWKQSDFLAVLPGVQCEAIDMKISPAGAIFVLARR
                     ASNNSMRWWLGEFATWGA"
BASE COUNT          299 a          673 c          902 g          283 t
ORIGIN      
        1 agcccgcgac agcggagctg atgccgatga ccgccgcgat cgaggccgcg tcgccgagtc
       61 ttcgccgctg cgccgggctc gcgggcgaga ccctcgtggt caacttctcc gcggctcggg
      121 gccgcgatag gttcgaggcc gtgacggtcc tcggaggcca cgacgacgag gtcgagcgct
      181 gtgtccgtga ggcgggggcg gcgatccgct tcgcgccggc gacgtcgcag tcgatgatga
      241 aggagtacgt gccatgaaga cgctcgcctg cttgctcgcc gtatcgctcg cgggggagag
      301 cccggcgccg actcaagttg ccggcgagac cgagtgcgtc gccaccgaca accgctgcca
      361 ggcgaagcgc ttcgagcaga aggccagccg ggcggagacg ccgaagatgc gggcgctcta
      421 cctgcacgcg gcgcatgtcg cccacctggc cgtcttcgac gagacggggg atgtgggggc
      481 cctgtgtgcg gcgcggcgga cattcgacaa gagcctggcg atcgaggggc agccagaggg
      541 gcagcggacg gccttcgagg atgcgcgcgg gaagttggag tcgcgcgaga agcagcgcgg
      601 ggctcgatgc ggatcggcgc ggcgctcggc caagacccag gcgcgagcgt tggcggcaaa
      661 ggtcgaggcg gcggccggtg ctggcgaggc ggagccgccg cccttgttgg cgatggggcc
      721 cgccgcgggc gcgcaggtgg aggacagcgt gagcgcgtcg ccgagcgagg cggtcacgtc
      781 ggcggcagcg gtggccccca gcgaggacac gctgctgccg gtgcggtcga gcgccgcggc
      841 atcgtcggcg agctcgacga cgccggctgt ggatccgcgg agtgtcggcg cttcgcgtgc
      901 gccgggtcgc cccctgctgt tcgcgggcag cgtgacgctg gcgatcgggc tctcgctcgg
      961 cggtgtcgcg gcctacaccg ggcgtcgtgc gctcgtggcg taccaggcag gtcttgacct
     1021 ccacgaggat gtgcagggcc cgcccgacgc tgcgacgctg gcgcgagacg ccgagctcga
     1081 ggccgagtac cggcggatgg gaccggtggc gatcgggacg gcgatcggcg gcggcgtgac
     1141 ggtggtcgtc ggggccgtga tggccggcgt cgggggccgg cggctcgcgc gggcgggctc
     1201 gcagaccgcc tgggcgcccg ttcccggggg ccttgcgttt catgtgcgat tttgagccaa
     1261 tccaggagtc agccatggcc acgccgaacc gaacccagct ctcgctcgtg ctcgtgaccc
     1321 tgctgtcggc ctgcgcgccg tggccggagt ggccggcggc cgagtgctcc gacaccagtt
     1381 gcgcgagctc gacggacacg agccctggca gcggcgggtc cttcggcgag gtcacgggga
     1441 ccgtgaccgg ggtgacgagc ggggcgagcg acgacgggac ggcggcgggg agcgacagcg
     1501 cgacggtggg tgagaccggc gaggagcaga cgaccggcgc gacgaccggc gacccgatcg
     1561 agccgccggc gatcgtcgag gtcgagctga cgccggaccc gatcatcttc aacgggccga
     1621 tcgcggtgac ggtgaacgcc gagcacgccg agggcgtgcg gatgaagctc gaggacggcg
     1681 aggaggtcga gctggtggag gcggagcccg gcgtgttcgt cggggagatc gccgtgatga
     1741 ccgggctcct gaacggcgac cgcgtggtgg tggtcacgcc gtgggcgggc gagctgatgg
     1801 gcgaggcggt ggaggcgagc tacacgatcg ccctgccggc gccggggagc gaggcgttct
     1861 gggaggccgg cgatctgatc ggcggcgggc aggtcgtgtc gatgggcgtg ctgccggacg
     1921 gcaacatcgt ggagttcggg acgcagtgga tccagggcgg ctcgcgctgc tacgtgcggc
     1981 ggcgcgacaa gagcggcgcg tggaagcaga gcgacttcct ggccgtgctg cccggcgtcc
     2041 agtgcgaggc catcgacatg aagatctcgc cggccggggc gatcttcgtg ctggcgaggc
     2101 gggccagcaa caacagcatg cggtggtggc tcggggagtt cgccacctgg ggcgcga
//
LOCUS       DAOQNN010000507         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_42019_length_2157_cov_5.886299, whole genome shotgun sequence.
ACCESSION   DAOQNN010000507 DAOQNN010000000
VERSION     DAOQNN010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42019_length_2157_cov_5.886299"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..>2157)
                     /locus_tag="PKV97_16720"
     CDS             complement(106..>2157)
                     /locus_tag="PKV97_16720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB07579.1"
                     /translation="RHARQGEELGRARRRLEKLVGHFPGMAYQFLLRADGSTSMPYCS
                     PGVRELYGVGPEGLQDSAMSLFERVHADDLDRLRDSILRSAANLAPWRCEFRVRGEGD
                     RLRWLLGEANPERTPEGDTLWHGYVHDITERQVAAQQLRESEARLRQAVDAVRDGLWS
                     WDLARDRIALDERILEMLDRPELGSEMCFDDFCALVHPADRQRQDAMLRAVPSATPDA
                     PVAGDLRLRAASGRWVWIHARGSVVETDAQGGPRRLVGTFSDITARVAATQLRRALLD
                     HSAAAIAMLDADLDILEANSRAHEIFAPPSTPIGGLKLTDLALHEDQAIFIPQHYAAL
                     RAGGQVNIELPLRDNRGKKRWFDVHGVMLDPEDPDSDTVWTLVDISDKYRTRMALATE
                     RLRLKTVLERFPGGVLMEDQDGLISSVNRGFCELLGLAAAPDELIGLTHEALCERLGT
                     ERTGWLHRPDDDRAAEKRATIEVEGAQGRTLEVDWLPIEHDGRRLGRVWLVRDVTERK
                     ERERRLAELAATDPLTGLPNRRSFLACLDAALDDARREPTRRSALLMIDIDHFKHVND
                     THGHPVGDEVLQHAARLIRGGLRQHDRAGRLGGEEFAVLLDDVDADTALTLAERLRRS
                     VEAAPAATAAGAVSLTISLGLALVSGGDPARVLGDADAALYRAKRGGRNRVCVAVRTP
                     G"
BASE COUNT          287 a          739 c          808 g          323 t
ORIGIN      
        1 gtcaggtctc cccttgggtg aaagtggcgg ggagtatagc cagcgcgggc gggatggcgg
       61 aaccgccgca tgcgcgtggt gcttcgatgc cggcggcgcg ctggtctatc ccggggtgcg
      121 aactgcgacg cagacacggt tgcggccgcc gcgcttggca cggtagagcg cggcatcggc
      181 gtcaccgagg acgcgggcgg gatcccctcc cgacacgagg gcaagaccga ggctgatggt
      241 gagcgacacc gcgccggccg cggtcgccgc gggcgccgcc tccacgctac ggcgcaggcg
      301 ttcggcgagg gtgagggcgg tgtcggcgtc gacgtcgtcg agcagcacgg cgaattcctc
      361 gccgccgagg cggcccgccc ggtcgtgctg gcgcaggccg ccgcggatca gccgtgccgc
      421 gtgctgcaat acctcgtcgc cgaccgggtg cccgtgggtg tcattgacgt gcttgaagtg
      481 gtcgatgtcg atcatcagca gggcgctgcg gcgcgtcggc tcacgccgcg catcgtccag
      541 cgcggcgtcc aggcaggcca ggaagctgcg ccggttgggc agtccggtca gcgggtcggt
      601 ggcggcgagc tccgccagcc ggcgttcgcg ctccttgcgt tcggtgacgt cgcgcaccag
      661 ccatacacgg ccaaggcgtc gcccgtcgtg ttcgatcggc agccagtcga cctccagcgt
      721 gcgcccctgc gcgccctcca cctcgatcgt ggcgcgcttc tccgccgcgc gatcgtcgtc
      781 cggccgatgc agccagcccg tgcgctcggt gcccagccgc tcgcacagcg cctcgtgcgt
      841 gaggccgatc agttcgtccg gggcggccgc gagaccgagc agttcgcaga acccccggtt
      901 caccgagctg atcaggccgt cctggtcttc catcaggacg ccgcccggga agcgctcgag
      961 caccgttttc aggcgcaggc gctcggtggc gagcgccatc cgcgtgcggt acttgtcgga
     1021 gatgtcgacc agggtccaga cggtgtcgct gtcggggtcc tccgggtcca gcatcacgcc
     1081 atgtacgtcg aaccagcgtt tcttgccacg attgtcgcgc aagggcagtt cgatgttgac
     1141 ctgtccgccg gcccgcagcg ccgcgtagtg ctgcgggatg aagatggcct ggtcctcgtg
     1201 cagggcgagg tcggtgagct tgagtccgcc gatcggggtc gagggcggcg cgaagatctc
     1261 gtgggcgcgg ctgttggctt cgaggatgtc gaggtcggcg tcgagcatgg cgatggcggc
     1321 cgcgctgtgg tcgaggaggg cccgacgcaa ctgcgtggcg gcgacccgtg cggtgatgtc
     1381 ggagaaggtg cccacgagac gccgcgggcc accctgcgcg tcggtctcca cgacgctgcc
     1441 gcgggcgtgg atccagaccc agcgcccgga cgcggcgcgc aggcggaggt cgcccgccac
     1501 gggagcgtcg ggcgtcgcgc tcggcacggc gcgcagcatc gcgtcctggc gctggcgatc
     1561 ggccggatgg acgagcgcgc aaaaatcgtc gaagcacatc tccgagccca gttccggacg
     1621 atcgagcatc tcgaggatgc gctcgtcgag cgcgatgcgg tcgcgggcga gatcccacga
     1681 ccacaggccg tcgcggacgg cgtcgaccgc ctggcgcagg cgcgcctcgc tttcgcgcaa
     1741 ctgctgcgca gccacctggc gctcggtgat gtcgtgcaca tagccgtgcc acagcgtgtc
     1801 gccttcgggc gtgcgctcgg ggttggcttc gccgagcagc cagcgcagcc ggtcgccctc
     1861 gccccgcacg cggaattcgc agcgccaggg cgcgaggttc gcggccgagc ggaggatgct
     1921 gtcgcgcaga cggtccagat cgtcggcgtg gacgcgctcg aacaggctca tggccgaatc
     1981 ctgcagcccc tcgggcccca ccccgtagag ttcgcgcacg cccgggctgc agtagggcat
     2041 ggaggtgctg ccgtccgcac gcagcaggaa ctggtaggcc atgcccggaa agtgaccgac
     2101 cagcttctcc agccgcctgc gggcgcggcc gagctcttcg ccctggcggg cgtggcg
//
LOCUS       DAOQNN010000508         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_42374_length_2148_cov_7.747253, whole genome shotgun sequence.
ACCESSION   DAOQNN010000508 DAOQNN010000000
VERSION     DAOQNN010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42374_length_2148_cov_7.747253"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="PKV97_16725"
     CDS             <1..1777
                     /locus_tag="PKV97_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidotransferase 1, exosortase A
                     system-associated"
                     /protein_id="HNB07580.1"
                     /translation="SIIDLSTGQQPLFNEDGTVAVVFNGEIYNFQELVPELEALGHRF
                     HTRSDTEVIVHAWESWGEDCVLRFRGMFAFALWDRNKQTFFIARDRLGVKPFYYAILD
                     DGTLVFGSELKVLMQHPGLDRRIDPHAVEEYFALGYVAEPRTIFKGAKKLQPGESLCI
                     RRGQPIPAPKLYWDVRFTLDNPLSLEDATAELTERLCESVRLRMIADVPLGAFLSGGV
                     DSSAVVATMAGLSSDPVNTCSIAFDDPAFNESEFAKMVADRYQTRHFVETVKSDDFDL
                     IDTLAALYDEPYADSSAIPTYRVCQLARKHVTVALSGDGGDEGFGGYRRYRMHLMEEK
                     MRAALPMGVRRPLFGLLGRVYPKADWAPRVFRAKTTFQAMARDATEAYFHSVSILRGD
                     MRRKLFSAEFRNRLGGYDALEVFRRHAAAAGTDDALALIQYLDLKTYLVGDINTKVDR
                     ASMAHSLEVREPLMDHKLVEWLATLPSGLKIQGNEGKYLFKKAMEPLLPDDVLYRPKM
                     GFAVPLARWFRGPLKQRVRDAVLGETLAATGIFERSYLEHLVDAHQSGARDYSAPLWT
                     LLMFEAFVRNTAAGATLPVALAEAV"
     gene            1799..>2148
                     /locus_tag="PKV97_16730"
     CDS             1799..>2148
                     /locus_tag="PKV97_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB07581.1"
                     /translation="MRILHILDHSIPLHSGYTFRTAAILREQRALGWETFHLTSPKQG
                     ETKAMVEEIEGLRFHRTGVPTPASSGIAELRQIRAVQARIEQLAAELRPDILHAHSPV
                     LNAIPAIRAGRKLG"
BASE COUNT          353 a          787 c          658 g          350 t
ORIGIN      
        1 gtcgatcatc gacctctcca ccggccagca gccgctgttc aatgaagacg gcaccgtggc
       61 cgtggtcttc aacggcgaga tctacaactt ccaggaactc gtgcccgagc tcgaagccct
      121 gggccaccgt ttccataccc gcagcgacac cgaagtcatc gtgcatgcct gggaatcctg
      181 gggtgaggac tgcgtgctgc gcttccgcgg gatgtttgcg tttgcgctgt gggaccgcaa
      241 caagcagacc ttcttcatcg cccgtgaccg cctgggcgtg aaaccgtttt actacgccat
      301 tctcgacgac ggcacgctgg tcttcggttc cgagctcaag gtgctgatgc aacaccctgg
      361 cctggaccgc cgcatcgatc cccacgccgt cgaagaatat ttcgccctcg gctacgtggc
      421 cgagccgcgc accatcttca agggggcgaa gaagctccag ccgggcgaga gcctgtgcat
      481 ccgccgcggc cagccgatcc ccgcgcccaa gctctactgg gatgtgcgct tcacgctcga
      541 caacccgctg tccctcgaag acgccaccgc cgagctcacc gaacgcctgt gcgaatccgt
      601 tcgcctgcgc atgatcgccg acgtgccgct gggtgccttc ctgtcgggtg gggtggattc
      661 cagcgccgtg gtggcaacca tggccggcct gtcgtccgat ccggtgaaca cctgctcgat
      721 cgccttcgac gatccggcct tcaacgagtc cgagtttgcc aagatggtgg ccgaccgcta
      781 ccagacccgt cacttcgtcg agaccgtcaa gtccgacgac ttcgacctca tcgacaccct
      841 ggccgcgctc tacgacgagc cctacgccga cagctcggcc atccccacct accgggtgtg
      901 ccagctcgcg cgcaagcatg tcaccgtggc gctgtcgggc gacggcggcg acgagggctt
      961 cggcggctac cgccgctacc gcatgcacct gatggaagag aagatgcgcg ccgcgctgcc
     1021 gatgggcgtg cgccgaccgc tcttcggcct gctcggccgc gtctatccca aggccgactg
     1081 ggcgccgcgc gtgttccgcg ccaagaccac cttccaggcg atggcgcgcg acgccaccga
     1141 ggcctacttc cacagcgtct ccatcctgcg cggcgacatg cggcgcaagc tgttcagcgc
     1201 cgaattccgc aacaggctcg gcggttacga cgcgctcgag gtcttccgcc gccatgccgc
     1261 agccgccggc accgacgatg cgctcgcgct gatccagtac ctcgacctca agacctacct
     1321 ggtcggcgac atcaacacca aggtcgaccg cgccagcatg gcgcactcgc tcgaggtgcg
     1381 cgagccgctg atggaccaca agctggtcga gtggctggcg acgctgccct ccggcctcaa
     1441 gatccagggc aacgagggca agtacctgtt caagaaggcg atggagccgc tgctgcccga
     1501 cgacgtgctc taccgcccga agatgggctt cgcggtgccg ctcgcgcgct ggttccgcgg
     1561 cccgctaaag cagcgcgtgc gtgacgcagt gctgggcgag accctggccg ctacgggtat
     1621 cttcgagcgc agctacctcg agcatctggt cgacgcccac cagtccggcg cgcgcgacta
     1681 cagcgccccg ctgtggacgc tgctgatgtt cgaggctttc gtccgcaaca ccgcagccgg
     1741 cgccaccctg ccggtcgcgc tggccgaggc ggtctgaacg cgacgttccg aatactccat
     1801 gcgcatcctg cacatcctcg atcactcgat cccgctgcac agcggctaca ccttccgcac
     1861 cgcggcgatc ctgcgcgagc agcgcgcgct cggctgggag accttccacc tgacctcgcc
     1921 caagcagggc gagacgaagg ccatggtgga ggagatcgag ggcttgcgtt tccaccgtac
     1981 cggcgtgccg acgccggcga gcagcggcat cgccgagctg cgccagatcc gtgcggtgca
     2041 ggcgcgcatc gagcagctcg cggccgaact gcgccccgac atcctccacg cccactcgcc
     2101 ggtgctcaac gccattccgg ccatccgcgc cgggcgcaag ctcggcat
//
LOCUS       DAOQNN010000509         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_42519_length_2144_cov_7.295357, whole genome shotgun sequence.
ACCESSION   DAOQNN010000509 DAOQNN010000000
VERSION     DAOQNN010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42519_length_2144_cov_7.295357"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /gene="glgB"
                     /locus_tag="PKV97_16735"
     CDS             <1..1083
                     /gene="glgB"
                     /locus_tag="PKV97_16735"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HNB07582.1"
                     /translation="DWNTLIYNYGRAEVRNYLASNALYWIERFGADGLRVDAVASMLY
                     RDYSRREGEWVPNRFGGRENLEAIDFLRRMNHLVGTQRPEAITLAEESTAFPAVSRPP
                     SPDLQGGGLGFHYKWNMGWMNDLLAYLARDPVHRKHHHDQLRFSLAYAFSENFVLPLS
                     HDEVVHGKGSLLAKMPGDVWQKFANLRLLYGYMWAHPGKKLLFMGCEFAPWSEWNADA
                     SLPWELLGHAPHAGMQRLVRDLNRVLRSHPALYEQDTASEGFRWISHDDAAHSVLVFE
                     RRARDGRSVVVACNFTPVAREGWRIGVPAAGTWRELLNTDDPVYGGSGVTNGTLPSAA
                     LPWQGEAQSLTLRLPPLGVAMFVRVD"
     gene            1214..>2144
                     /locus_tag="PKV97_16740"
     CDS             1214..>2144
                     /locus_tag="PKV97_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HNB07583.1"
                     /translation="MAHALPDPDLRPGQPWPLGATLTADSGETGVNFAVWSPDAEAIE
                     LCLFDAAGREALRRLALPACSEGVWHGFLPAGGVGLVYGLRAHGPWAPGRGHWFNPAK
                     LLLDPWAQEVVGSYGRQQPGAADDVELAVRLAAFRAARSDDPRLPDPRDNAAFAPKAR
                     VLAPAPAPARDRPPRPHIPRERTVLYEAHVRALTMRHPDIPPALRGSYAALAHPVMLD
                     HYRALGLTTLSLLPLHFRADEAALQRRGLANHWGYAPAAWLAPEPRYWSGRAGTTPTG
                     ELAGAIDALHAAGLEVVLDVVFNHTAELDADGPM"
BASE COUNT          297 a          807 c          734 g          306 t
ORIGIN      
        1 gactggaaca cgctgatcta caactacggc cgcgccgagg tgcgcaacta cctcgccagc
       61 aacgcgctgt actggatcga gcgcttcggc gccgacgggc tgcgggtgga tgcggtggcc
      121 tcgatgctgt atcgcgacta cagccggcgc gagggcgagt gggtgccgaa ccgcttcggc
      181 gggcgcgaga acctggaggc gatcgacttc ctgcgtcgca tgaaccacct cgtcggcacg
      241 cagcgccccg aggcgatcac gctcgccgag gaatccaccg ccttccccgc cgtcagccgc
      301 ccgccctcac ccgacctgca gggcggcggc ctgggcttcc actacaagtg gaacatgggc
      361 tggatgaacg acctcctcgc ctacctcgcg cgcgacccgg tgcatcgcaa gcaccaccac
      421 gaccagctgc gcttctcgct ggcgtatgcc ttcagcgaga acttcgtgct gccgctgtcg
      481 cacgacgagg tggtgcacgg caagggctcg ctgctcgcca agatgcccgg cgacgtctgg
      541 cagaagttcg ccaacctgcg cctgctctac ggctacatgt gggcgcaccc gggcaagaag
      601 ctgctcttca tgggttgcga gttcgccccg tggtccgaat ggaacgccga cgccagcctg
      661 ccctgggagc tgctcgggca cgccccgcac gcgggcatgc agcgcctggt gcgcgatctc
      721 aaccgcgtgc tgcgcagcca tccggcgctg tacgagcagg acacggcgag cgaaggcttc
      781 cgctggatct cccacgacga tgcggcgcac tcggtgctgg tcttcgagcg ccgcgcgcgc
      841 gacggccgca gcgtggtggt cgcgtgcaac ttcaccccgg tggcgcgcga gggctggcgt
      901 atcggcgtac ccgcggcggg cacctggcgc gagcttctca acaccgacga tccggtgtat
      961 gggggctcag gcgtcaccaa cggcacgctg cccagcgcgg cgctgccctg gcagggcgag
     1021 gcgcagtcgc tcaccctgcg cctgccgccg ctgggcgtgg cgatgttcgt acgcgtggac
     1081 tgacacgcct ggacgcatgc cccggcaaga gaacgcagcg atgagcgccc cgcaccccga
     1141 ctcccttccc atcctgcagc acgggtgcgc tccttgtgag gacgggcccg gcgccgcagc
     1201 ggatccgctc gccatggcgc atgcgctgcc tgatccggac ttgcgcccgg ggcagccgtg
     1261 gccgctcggt gccaccctca ccgcagacag cggcgagacc ggggtgaact tcgcggtgtg
     1321 gtcgccggac gccgaagcga tcgagctctg tcttttcgat gccgcaggcc gcgaggcgct
     1381 tcgccgcctg gcgctgccgg cgtgcagcga aggcgtctgg catggctttt tgcctgccgg
     1441 cggggtcggc ctggtgtatg gcctgcgtgc tcatggcccg tgggcgccag ggcgcggcca
     1501 ctggttcaac ccggcgaagc tgctgctcga cccctgggcg caggaggtgg tcggcagcta
     1561 cggccgccag cagcccggag cagccgacga tgtggagctc gcggtaaggc tcgccgcgtt
     1621 ccgcgccgcc cgcagcgacg atccgcgcct gcccgacccg cgcgacaacg ctgccttcgc
     1681 ccccaaggcg agggtgctcg cgcccgcccc tgctccggcg cgtgaccgcc ctccccgccc
     1741 gcacatcccg cgcgagcgca cggtactcta cgaggcccac gtgcgtgcgc tgacgatgcg
     1801 ccacccggac atcccgccgg cgctgcgcgg cagctacgcc gccctcgccc acccggtgat
     1861 gctcgatcac taccgggcgc tcggcctcac cacgctgagc ctgctgcccc tgcacttccg
     1921 cgccgacgaa gcggcgctgc agcgccgtgg cctcgccaac cactggggct atgcgcccgc
     1981 agcctggctc gcccccgagc cgcgctactg gagcgggcgc gcgggcacca ccccgaccgg
     2041 ggaactcgcg ggggcgatcg acgcgctgca cgccgccggg ctcgaggtcg tgctcgacgt
     2101 ggtcttcaac cacacggccg agctcgacgc ggacggcccg atgc
//
LOCUS       DAOQNN010000510         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_42645_length_2140_cov_14.254197, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000510 DAOQNN010000000
VERSION     DAOQNN010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42645_length_2140_cov_14.254197"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PKV97_16745"
     CDS             complement(<1..536)
                     /locus_tag="PKV97_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNB07584.1"
                     /translation="MSDQNSDNSKQENPGGLWARLRRPSGKSLGGLLAVGFMVGVLFW
                     GGFNTALEATNTEKFCISCHEMYDNVYMEYKETIHYNNRTGVRATCPDCHVPKDWTHK
                     MIRKVQASKELWGKMVGTIDTREKFEAKRLQLARNEWKRMKAADSRECRNCHSFESMN
                     PEAQKQRARKQHEMAKED"
     gene            complement(605..>2140)
                     /locus_tag="PKV97_16750"
     CDS             complement(605..>2140)
                     /locus_tag="PKV97_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNB07585.1"
                     /translation="CHGVLRKGATGKNLEPHWTKTAPDGTKLEGGTLKLGTERLEKII
                     SYGTEGGMVNYDDILTKEEINLMARYIQNTPPIPPEFSLKDMKDSWKLLVPVKDRPTK
                     QMNKVNLKNVFAITLRDAGKLALVDGDTHKIWKILDTGYAVHISRLSASGRYVYTVGR
                     DGLTTIIDMFYEEPTTVATVRLGSDARSVDTSKFKGYEDKYLIGGTYWPPQYSIMDGE
                     TLEPMKIVSTRGNTVDGDYHPEPRVASIVASMTKPEWVVNVKETGQILLVDYSDIKNL
                     KSIAIESAKFLHDGGWDMSKRYFMVAANASNKVAAVDTKEGKLAALVDTAKIPHPGRG
                     ANFVHPKFGPVWSTGHLGDAVVSLISTASDDPKFAKYKEHNWKVVEQLKMPGAGNLFV
                     KTHPKSKHFWADAPMNPEREVAESVYVFDMNDLSKAPVALNVAKDSGLPESKAIRRAV
                     QPEYNEAGDEVWISLWGGKTDQSAIVIYDDKTLKVKKVITDPAIVTPTGKFNVYNTMH
                     DVY"
BASE COUNT          334 a          658 c          667 g          481 t
ORIGIN      
        1 ttgtcttcct tcgccatctc gtgctgcttg cgcgcacgct gcttctgggc ctcgggattc
       61 atgctctcga aactgtggca gttgcggcac tcgcgcgagt cggccgcctt catccgcttc
      121 cactcgttgc gcgccagttg caggcgcttg gcctcgaact tctcgcgcgt gtcgatggtg
      181 ccgaccatct tgccccacag ttccttggac gcctgaacct tgcggatcat cttgtgcgtc
      241 cagtctttgg gcacgtggca gtccgggcag gtcgcgcgca cgccggtccg gttgttgtag
      301 tgaatggttt ccttgtactc catgtacacg ttgtcataca tctcgtggca cgaaatacag
      361 aacttctcgg tgttcgtggc ctccagcgcg gtgttgaagc cgccccagaa caacacgccc
      421 accatgaatc caacagccaa caggccgccc agcgacttac cggaaggacg gcgcaggcgt
      481 gcccacaggc cgcccggatt ttcttgtttg gagttgtcgc tgttttgatc agacatggaa
      541 ctcttcccct cgatcccccg cgccaggggc gcgggggttt ttcagagctt gaccggaaga
      601 tcgatcaata gacgtcgtgc atcgtgttgt acacgttgaa cttgccggtc ggcgtgacga
      661 tggccgggtc ggtgatcacc ttcttgacct tcagcgtctt gtcgtcgtag atcacgatcg
      721 cggactggtc ggtcttgccg ccccacagcg agatccacac ttcgtcgcct gcctcgttgt
      781 actccggctg cacggcgcgg cggatggcct tgctctcggg caggcccgag tccttggcga
      841 cgttgagcgc gaccggcgcc ttcgacagat cgttcatgtc gaacacgtac accgactcgg
      901 ccacttcacg ctccgggttc atcggcgcat cggcccagaa gtgcttcgac ttcgggtgcg
      961 tcttgacgaa caggttgccg gcgcccggca tcttcagctg ttcgacgacc ttccagttgt
     1021 gctccttgta cttggcgaac ttgggatcgt cggaagcggt cgagatcagc gagaccacgg
     1081 catcgccgag gtgacccgtc gaccacaccg gaccgaactt cggatggacg aagttggcgc
     1141 cacgccccgg gtgcgggatc ttggcggtat cgaccagcgc agccagcttg ccttccttgg
     1201 tgtcgaccgc ggcgaccttg ttggacgcgt tggcggcgac catgaagtag cgcttcgaca
     1261 tgtcccagcc gccatcgtgc aggaacttgg cggactcgat cgcgatgctc ttcaggttct
     1321 tgatgtcgga gtaatcgacc agcaggatct ggccggtttc cttgacgttg accacccact
     1381 ccggcttcgt catcgaggcc acgatggagg ccacgcgcgg ctcggggtga tagtcgccat
     1441 cgacggtgtt gccacgggtc gacacgatct tcatcggctc gagcgtctcg ccgtccatga
     1501 tcgagtactg gggcggccag taggtgccac cgatcaggta cttgtcctcg tagcccttga
     1561 acttggaggt atcgaccgaa cgggcatccg aacccaggcg cacggtggcg accgtggtgg
     1621 gctcttcgta gaacatgtcg atgatggtgg tcaggccgtc acgaccgacg gtgtagacgt
     1681 agcggcccga agccgacagg cgggagatgt gcaccgcgta gccggtatcg aggatcttcc
     1741 agatcttgtg ggtgtcgccg tcgaccagcg cgagcttgcc cgcgtcgcgc agcgtgatcg
     1801 cgaacacgtt cttcaggttg accttgttca tctgcttggt cggacggtcc ttgacgggga
     1861 ccagcagctt ccagctgtcc ttcatgtcct tgagcgagaa ctcgggcggg atcggaggcg
     1921 tgttctggat gtaacgcgcc atcaggttga tctcttcctt ggtcaggatg tcatcgtagt
     1981 tgaccatgcc gccttcggtg ccgtacgaga tgatcttctc gaggcgctcg gtgccgagct
     2041 tgagggtgcc gccctccagc ttggtgccgt ccggagcggt cttggtccag tgcggctcga
     2101 ggttcttgcc ggtggcgccc ttgcgcagca cgccgtggca
//
LOCUS       DAOQNN010000511         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_42720_length_2138_cov_7.400864, whole genome shotgun sequence.
ACCESSION   DAOQNN010000511 DAOQNN010000000
VERSION     DAOQNN010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42720_length_2138_cov_7.400864"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PKV97_16755"
     CDS             complement(<1..394)
                     /locus_tag="PKV97_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNB07586.1"
                     /translation="MNALASSPAVLLATLAFGLASALLAWSAMRIAGNALARYRERFT
                     AEAHLQLEELFLFMDPARLFVLNLLAVTVGGIGSWLASGEVLIALASAGALALLPRLA
                     FGLLRRRRLDLIEQQLPDALQVIAGGLRA"
     gene            complement(391..1815)
                     /locus_tag="PKV97_16760"
     CDS             complement(391..1815)
                     /locus_tag="PKV97_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNB07587.1"
                     /translation="MFFRTARKPPEDSSTTLAAAADTPAATSAPVPEPPFRPGVEPHP
                     AAAEANADFTWRKRIHERLLDTIDLRRRDLNRMSDDELRAETTALVREIIAAEAALPG
                     GLDREQLCSEVLDEAIGLGPLETLLADESVSEIMVNRFDQIFVERGGRIAPHPTTFTS
                     DRAVLGVIERIVAPLGRRIDESSPMVDARLRDGSRVNAIIPPLALKGPTLTIRKFARR
                     ALEVADLVRMGSLSHEMAAFLRTCVEQRRNVVVSGGTGSGKTTFLNLLSNFIPDGERI
                     ITIEDAAELRLRHSHLVSLEARPSNLEGRGAISIRDLVRNALRMRPDRIVVGECRGGE
                     ALDMLQAMNTGHEGSMTTLHANSPRDALARLETLVLMAGMDLPLAAIREQIASAVDII
                     VQQTRFACGARRLTSITELTGMEGGRIQLQELFRFERRSADSPTADPSGGHFTGCDAV
                     PGFYDELRRQGVALDLHLFDRKRS"
     gene            complement(1828..>2138)
                     /locus_tag="PKV97_16765"
     CDS             complement(1828..>2138)
                     /locus_tag="PKV97_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HNB07588.1"
                     /translation="RLRDELETLLKREQQSDENTVPYLGEVPVLGRLFRSTRFNNRES
                     ELVVFVTPWLLEPGEGLLDPRAQLLERRAEQALAPTPEPSPALREPADPAWNALYGGN
                     "
BASE COUNT          305 a          659 c          844 g          330 t
ORIGIN      
        1 ccgcgcgcag gccgccggcg atcacctgca gcgcgtcggg cagctgctgt tcgatgaggt
       61 cgagccggcg ccggcgcagc agtccgaagg caagacgcgg tagcagggcc agcgcacccg
      121 cgctggcgag tgcgatcagc acctcgccgc tggcgagcca gctgccgatg ccgccgacgg
      181 tgaccgcgag gaggttcagg acgaagagtc gcgccgggtc catgaacagg aagagttctt
      241 cgagctgcag gtgggcctcg gcggtgaagc gttcgcggta gcgtgcgagc gcattgcccg
      301 cgatgcgcat tgccgaccag gcgagcaggg cggaggcgag gccgaaggcg agcgtggcca
      361 gcagcacggc gggtgacgaa gccagggcat tcatgaccgc ttccggtcga acaggtgcag
      421 gtcgagcgcg accccttggc ggcgcagttc gtcgtagaag cccggcacgg catcgcagcc
      481 ggtgaagtgg ccgccgctcg ggtcggcggt gggggaatcg gcgctgcggc gctcgaagcg
      541 gaagagttcc tgcagctgga tgcgtccgcc ctccatgccg gtgagctcgg tgatgctggt
      601 gagccggcgc gcgccgcagg cgaagcgggt ctgctggacg atgatgtcca ccgcgctggc
      661 gatctgctcg cggatcgcgg ctagcgggag atccatgccg gccatcagga cgagcgtctc
      721 gaggcgggcg agcgcatcgc gcgggctgtt cgcgtgcagg gtcgtcatcg agccctcgtg
      781 gccggtgttc atcgcctgca gcatgtcgag cgcctcgccg ccgcggcatt cgcccaccac
      841 gatgcggtcg gggcgcatgc gcaacgcgtt gcgcacgagg tcgcggatgc tgatcgcgcc
      901 gcgcccttcc aggttggacg ggcgcgcctc caggctgacc aggtggctgt ggcgcaggcg
      961 cagctcggcg gcgtcctcga tggtgatgat gcgttcgccg tcggggatga agttcgacag
     1021 caggttgagg aaggtggtct tgcccgagcc ggtgccgccg gagaccacga cgttgcgccg
     1081 ctgctcgacg caggtgcgca ggaatgcggc catctcgtgc gagagcgaac ccatgcggac
     1141 gaggtccgcg acctcgagcg cccgacgcgc gaacttgcgg atggtgagcg tcgggccctt
     1201 gagggcgagc ggcgggatga tcgcattgac ccgcgagccg tcgcgcagcc gggcatcgac
     1261 catcggcgag gattcgtcga tgcggcggcc gagcggcgcg acgatgcgct cgatgacgcc
     1321 gagcaccgcg cggtcgctgg tgaaggtggt ggggtgcggc gcgatgcggc cgccacgttc
     1381 gacgaagatc tggtcgaagc gattgaccat gatctcgctc accgactcgt cggcgagcag
     1441 ggtctcgagc ggtccgaggc cgatggcctc gtcgagcacc tcgctgcaca gctgttcgcg
     1501 gtcgagcccg cctggaagcg cggcctcggc ggcgatgatc tcgcgtacga gcgcggtggt
     1561 ctcggcgcgc aactcgtcgt ccgacatgcg gttgagatcg cgccggcgca ggtcgatggt
     1621 gtcgagcagg cgttcgtgga tgcgtttgcg ccaggtgaag tccgcgttcg cttcggcagc
     1681 tgccgggtgc ggttcgacgc ccgggcggaa cggtggctcg ggtacgggcg cggacgttgc
     1741 ggccggcgta tccgccgctg cggccagggt ggtggacgag tcttcgggcg gtttgcgtgc
     1801 ggtgcggaaa aacatgcagg cctccggtca gttgccgccg tagagggcgt tccacgcggg
     1861 atccgcgggc tcgcgcagcg ccggcgaggg ttcgggcgtt ggggcgaggg cctgctcggc
     1921 acggcgttcg agcagttgcg cgcgcggatc cagaaggcct tcgccgggct cgagcagcca
     1981 cggggtgacg aagaccacga gttcgctttc gcggttgttg aagcgggtgg agcggaaaag
     2041 gcgccccagg acgggcactt cgcccaggta ggggaccgtg ttctcgtcgc tctgctgctc
     2101 gcgcttgagc agggtttcca gttcgtcgcg caggcgcg
//
LOCUS       DAOQNN010000512         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_42905_length_2133_cov_10.372474, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000512 DAOQNN010000000
VERSION     DAOQNN010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42905_length_2133_cov_10.372474"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1222)
                     /locus_tag="PKV97_16770"
     CDS             complement(143..1222)
                     /locus_tag="PKV97_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07589.1"
                     /translation="MLHAQASERELIDQFLAALRSLPEVQAELERVGSPELDAQLALD
                     VAGKPIHALVELRKAVYPRDVRQLVWRIRDLARQQPAGESGSEALPVLIAESISPGAK
                     ELLRAERVGYYDSGGSLYLPARGAYLYVDRPPPKSLSRSMRSLFTGRRAQVLHGLLVR
                     HQDWFGVKDLAEQTLVSSATASQVLTEMERFDWLESRGQGPSKQRHLREPAALLDAWA
                     KQLSSIRPPALRRYFVPAMKPDGLMERLGQVCEAHSVDYAVSFESAAQRYAPFLSAVS
                     QVRCRLLAGSGGDAAIADLGARVVSEGANLAVIEAKSAGDLLFRERVGGVWLASPVQV
                     YLDLLRGEGRAKEMAEHLRRERIGF"
     gene            1368..>2133
                     /locus_tag="PKV97_16775"
     CDS             1368..>2133
                     /locus_tag="PKV97_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07590.1"
                     /translation="MRRRGTPVSREALYEEVWTDAVTVVAPRYGLSDVGLVKICKKLG
                     IPVPPRGYWAKVKAGRPTRKVPLPALPAGARDLAGPIPLSEQEAAMHARVRDALQQTR
                     ESQSPVSVPAELVDPHPLVRATAARLKRRDGWDHPAGVRSAPKEVLDLQVTRNTLDRA
                     LLLMDTLLKSLEPSGFTARVDEEKGETLLVGGGTTLTISIVEQVTRTSHTPTRAEVRA
                     RDRYYDSFRVGARGEYPNIPQFDWHPTGRLTLTVGSW"
BASE COUNT          367 a          760 c          704 g          302 t
ORIGIN      
        1 accagatacc tcggcgtgag gccgccgacc aggtagaccg agtccttcca cggccccagg
       61 ccccgcagca gcgtcaccag cacccgctcg cagtcgagcg tgtactggtc gctgtaaccg
      121 tcgaaggttg cgggcttggc catcagaagc cgatcctttc tcgacgcagg tgttcggcca
      181 tctccttggc tcgaccctcg ccgcggagga ggtcgaggta cacctggacc ggactggcca
      241 gccagacccc gccgacccgc tcgcgaaaga gcaggtcacc ggccgacttc gcttcgatga
      301 cggcgagatt cgcgccctcg ctgacgacgc gtgcgcccag gtccgcgatc gcggcatcgc
      361 cccccgaccc cgccagcagt cggcatcgaa cctgcgatac cgccgacagg aacggcgcgt
      421 agcgctgcgc ggcgctctcg aaactgacgg cgtagtcgac gctatgcgcc tcgcaaacct
      481 gaccgaggcg ctccatcagg ccgtcgggct tcatcgcggg cacgaagtag cgtcgcagcg
      541 ccgggggccg gatcgagctc agctgcttcg cccaggcatc cagcagcgcc gcgggttccc
      601 gaaggtgcct ctgcttgctg gggccctgcc cgcgcgactc cagccagtcg aagcgctcca
      661 tctcggtgag cacctgggac gccgtggctg acgagaccag cgtctgctcg gccaggtcct
      721 tcacgccgaa ccagtcctgg tggcgaacca acaggccgtg caggacctgt gcgcgccgcc
      781 ccgtgaacag cgagcgcatc gagcgggaca acgacttggg cggcggccga tcgacgtaga
      841 ggtaggctcc ccgggccggc aggtagaggc tgcccccact gtcgtagtac ccgacccgct
      901 cggctcgaag caactccttc gcccccggcg agatggactc ggcgatcagc accggcagcg
      961 cctcgctgcc gctctcgccg gcgggttgct ggcgggcaag gtccctgatc cgccagacga
     1021 gctgacggac gtcgcgcgga tagacggcct tcctcaactc gacgagcgcg tggataggct
     1081 taccggcgac gtcgagcgca agctgggcgt cgagctccgg cgagccgacg cgttcgagtt
     1141 cggcctgcac ttcaggcagc gaccgaagtg cggcgaggaa ctgatcgatc agctcccgtt
     1201 cgctcgcctg cgcatgaagc atggtcaccg cctaggtttg ctgtgcagta aatatgcgca
     1261 ccataccgaa gattcggcct tatggcaata tttactgaac agcaaatctt cgaaccaaca
     1321 ggagccgccg tccccaagcg gctccggccc gaaccgggag acacgcaatg agacgccgag
     1381 gcacccccgt cagccgggag gcgctgtacg aagaggtctg gaccgacgcg gtcacggtcg
     1441 tcgccccccg ctacggcctg tccgacgtgg gcctggtcaa gatctgcaag aagctcggca
     1501 ttccggtgcc gccgcggggc tactgggcga aggtcaaggc cggtcgcccc acccgcaagg
     1561 tgccgctgcc cgccctaccc gccggcgcgc gcgatctcgc cggaccgata ccgctgtccg
     1621 aacaggaggc ggccatgcac gcgcgtgtcc gtgacgccct ccaacagacc cgcgagagcc
     1681 agtcgccggt cagcgtcccc gccgagctcg tcgacccgca cccgctcgta cgggccacag
     1741 cggcgcgctt gaagcggcgc gacggctggg atcacccggc gggcgtgcga agcgcgccga
     1801 aggaagtgct cgacctgcag gtcacgagga acaccctcga ccgcgccctt ctgctgatgg
     1861 acacgctgct caagtcgctc gagcccagcg gcttcacggc gcgggtggac gaggagaagg
     1921 gggagaccct gctggtcggc ggcggcacga cgctgacgat ctcgatcgtc gagcaggtca
     1981 cgcgaaccag ccacacgccg acacgcgcgg aggtgcgggc gcgcgaccgg tactacgact
     2041 cctttcgcgt gggtgcccga ggagagtatc cgaacatccc gcagttcgat tggcacccca
     2101 ccggacggct gacgctcacg gtgggatcgt ggc
//
LOCUS       DAOQNN010000513         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_43110_length_2127_cov_9.328668, whole genome shotgun sequence.
ACCESSION   DAOQNN010000513 DAOQNN010000000
VERSION     DAOQNN010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43110_length_2127_cov_9.328668"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="PKV97_16780"
     CDS             complement(<1..1899)
                     /locus_tag="PKV97_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HNB07591.1"
                     /translation="MKIERLPDEDATSSASARAAATRPAAPPTTATPLIELSGITRSF
                     VNGEVETRVLHGIDLTIHPGEFVAIMGTSGSGKSTLMNILGCLDRPTGGTYRFMGTDV
                     AALDRDALARLRREAFGFVFQSYNLLGGASARDNVEVPAVYSGMPREERHARAEALLA
                     RLGLGERIHHRPGQLSGGQQQRVSIARALMNGGRIIFADEPTGALDSRSGAEVMKLLA
                     ELSAAGHTIVLITHEREVAEQAQRIIEIRDGRIVADPGPHPPQEPEPDFAPHIDRTSP
                     LSDVVEATRTALRALRANLFRTVLTLLGIVIGVASVIAMLAIGDGAKQKVIDQVSAMG
                     TNLLTVRPGAPNTRGRDAPATLVIEDVQAIAELPNVLASVPEQGATITLRAGNIDHRS
                     SVNGTSADYVVARNWAPASGTFFSAEDEARYATVAVLGQTVARALFPGRDAVGEFILV
                     NNIPFQVMGVMTPKGATPWGQDQDDIVFMPFTTASLRITGQRFLRNVTVAVEDVGQID
                     DTQAAVSALLLARHGVEDFQIRNMASVIDTVSETQNTLTVLLGTVAAISLLVGGIGVM
                     NIMLVSVTERTREIGIRMATGARTRNILQQFLIEALVVSAVGGLIGVAVGLGAAAIIE
                     AFGTAVQYS"
     gene            complement(1896..>2127)
                     /locus_tag="PKV97_16785"
     CDS             complement(1896..>2127)
                     /locus_tag="PKV97_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07592.1"
                     /translation="MSQGRGAASGTSGAGPRQGTVRVADARGALEERKVELGVSNRVQ
                     AQVLSGLAEGERVVSGLVVGNGAGRPQMTPRL"
BASE COUNT          283 a          717 c          808 g          319 t
ORIGIN      
        1 cgagtactgc accgcggtgc cgaaggcctc gatgatcgcg gcggcgccga gcccgaccgc
       61 gaccccgatc agcccgccca ccgccgagac cacgagggcc tcgatgagga actgctgcag
      121 gatgttgcgc gtgcgcgcgc cggtggccat gcggatgccg atctcgcggg tgcgctcggt
      181 gaccgacacc agcatgatgt tcatcacgcc gatgccgccc accagcagcg agatcgccgc
      241 caccgtgccg agcagcacgg tgagcgtgtt ctgcgtctcc gacacggtgt cgatcaccga
      301 ggccatgttg cggatctgga agtcctccac gccgtggcgc gcgagcagca gcgcgctcac
      361 cgcggcctgg gtgtcgtcga tctggccgac gtcctcgacc gccacggtga cgttgcgcag
      421 gaagcgctgc ccggtgatgc gcaggctggc ggtggtgaag ggcatgaaca cgatgtcgtc
      481 ctggtcctgc ccccagggcg tggcgccctt gggcgtcatc acccccatga cctggaaggg
      541 gatgttgttc accaggatga actcgcccac cgcgtcgcgc cccgggaaca gcgcgcgcgc
      601 cacggtctgg ccgagcacgg cgacggtggc gtagcgcgcc tcgtcctcgg cgctgaagaa
      661 ggtgccgctc gccggcgccc agttgcgcgc cacgacgtag tcggccgagg tgccgttgac
      721 gctgctgcgg tggtcgatgt tgccggcgcg cagggtgatg gtggcgccct gctcgggcac
      781 cgaggcgagc acgttgggca gctcggcgat cgcctgcacg tcctcgatca ccagggtggc
      841 gggggcgtcg cgtccgcgcg tgttgggtgc gccggggcgc acggtgagca ggttcgtgcc
      901 catcgcgctg acctggtcga tcaccttctg cttggcgccg tcgccgatcg ccagcatggc
      961 gatcaccgag gcgacgccga tgacgatgcc gagcagggtc aggacggtgc ggaagaggtt
     1021 ggcgcgcagc gcgcgcagcg cggtgcgggt ggcctccacc acgtccgaca gcggcgaggt
     1081 gcgatcgatg tggggggcga agtcgggctc cggctcctgc ggcgggtgcg gcccggggtc
     1141 ggcgacgatg cggccgtcgc ggatctcgat gatgcgctgc gcctgctcgg cgacctcgcg
     1201 ctcgtgggtg atcagcacga tggtgtggcc ggcggcggag agctcggcga gcagcttcat
     1261 gacctcggcg ccgcttcggc tgtcgagcgc gccggtgggc tcgtcggcga agatgatgcg
     1321 gccgccgttc atcagcgcgc gcgcgatcga cacgcgctgc tgctggccgc cggagagctg
     1381 gccggggcga tggtggatgc gctcgccgag cccgaggcgc gccagcagcg cctcggcacg
     1441 ggcatggcgt tcctcgcgtg gcatgccgga atacaccgcc ggcacctcga cattgtcgcg
     1501 cgcggaggcg ccgccgagca ggttgtagct ctggaagacg aagccgaagg cctcgcggcg
     1561 caggcgcgcg agcgcgtcgc ggtccagcgc cgcgacgtcc gtgcccatga agcggtaggt
     1621 gccgccggtg gggcgatcga ggcagccgag gatgttcatc agcgtggatt tgcccgaacc
     1681 cgaggtgccc atgatggcga cgaactcgcc agggtggatg gtgaggtcga tgccgtgcag
     1741 cacgcgggtc tcgacctcgc cattgacgaa gctgcgcgtg atgcccgaga gctcgatcag
     1801 cggtgttgcg gtcgtgggcg gggcggcggg ccgcgtggcc gctgcgcgcg cgctggcgga
     1861 cgaggtcgcg tcctcgtcgg gaaggcgttc gatcttcata gccgcggcgt catctgcggg
     1921 cgaccggcgc cgtttccgac caccagcccg gagacgacgc gttcgccctc ggccagcccg
     1981 gacagcacct gtgcctgcac gcggttggag acgccgagtt cgaccttgcg ttcttccagc
     2041 gcgccgcgtg catcggcgac ccgcaccgta ccctggcgcg gtccggcgcc ggaggtgccc
     2101 gacgctgcgc cgcgcccctg gctcatc
//
LOCUS       DAOQNN010000514         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_43210_length_2124_cov_7.813436, whole genome shotgun sequence.
ACCESSION   DAOQNN010000514 DAOQNN010000000
VERSION     DAOQNN010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43210_length_2124_cov_7.813436"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..867)
                     /locus_tag="PKV97_16790"
     CDS             complement(34..867)
                     /locus_tag="PKV97_16790"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNB07593.1"
                     /translation="MCQLLGMNCNVPTDICFSFAGFRARGGLTDHHRDGWGIAFFEGR
                     GVRVFLDPAPSAHSPVAELVNRYPIRSLNVIAHIRKATQGEIRLENTHPFQRELWGRY
                     WIFAHNGNLKDFAPPLSGRFLPVGSTDSELAFCHILDTLAARFPAGKPAPAELHAALR
                     ELAREVGSRGEFNFLLSDGDWLFAHCSSRLCYIVRRAPFAEAHLADEDLTVDFRRLTG
                     PQDRVAVIATTPLTDNEHWTQIAPGNLVAFRDGDTIALGETATVAQDYPRRGIDKAPS
                     A"
     gene            complement(901..>2124)
                     /gene="gatB"
                     /locus_tag="PKV97_16795"
     CDS             complement(901..>2124)
                     /gene="gatB"
                     /locus_tag="PKV97_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNB07594.1"
                     /translation="RKNYFYPDLPKGYQISQYELPVVQGGHLDLLVPDGKGGHYEKRV
                     NLTRAHLEEDAGKSLHEDFHGMTGIDLNRAGTPLLEIVSEPDMRSSAEAVAYARALHA
                     LVRWIDICDGNMQEGSFRCDANVSVRRKGATEFGTRREIKNLNSFRFLQQAIDYEVQW
                     QIDTLEDGGRVVQATVLFDPDTGETRMMRSKEDAHDYRYFPDPDLLPLMISPEWKARV
                     QAEMPELPGAMKARFMDDWGLSAYDATTLTASKEVADFYQATVSAAGAALAKPCANWV
                     MGELAARLNKAELDVAASPVSPAQLAGLVARIADNTISNAIARKVFEALWNGEGATAD
                     EVIDKQGLRQVTDTGAIEAMIDEVLAANQKSVEEFRAGKEKAFNALVGQVMKASKGKA
                     GPAQVNALLRKKLGA"
BASE COUNT          294 a          696 c          769 g          365 t
ORIGIN      
        1 ctaggcccga cgctgcgggc gggcgaggaa ctgttacgca ctcggagcct tgtcgatgcc
       61 ccggcgcggg taatcctgcg ccacggtggc ggtttccccg agcgcgatcg tgtcgccgtc
      121 gcggaaggcg accaggttgc ccggcgcgat ttgcgtccag tgctcgttgt cggtgagcgg
      181 cgtggtggcg atcaccgcga cgcggtcctg cggaccggtg aggcggcgga aatccaccgt
      241 gaggtcctcg tcggccaggt gggcctcggc gaagggcgcg cgccggacga tgtagcacag
      301 ccgcgacgag cagtgcgcga acagccagtc gccgtccgag agcaggaagt tgaattcgcc
      361 gcggctgccg acctcgcgcg cgagctcgcg cagcgccgcg tgcagttccg cgggcgcggg
      421 cttgcccgcg ggaaagcgcg cggccagcgt gtcgaggatg tggcagaagg cgagctccga
      481 gtcggtgctg ccgaccggca gaaagcgccc cgagagcggc ggcgcgaagt ccttcaggtt
      541 gccgttgtgg gcgaagatcc agtagcgtcc ccacagctcg cgctggaagg ggtgggtgtt
      601 ctcgagccgg atctcgccct gggtggcctt gcggatgtgg gcgatgacgt tgagcgagcg
      661 gatcgggtag cgattcacca gctccgccac cggcgaatgc gcgctcggcg ccgggtcgag
      721 gaagacgcgc acgccgcggc cctcgaagaa ggcgatcccc cagccgtcgc ggtggtggtc
      781 ggtgagcccg ccgcgcgcgc ggaagcccgc gaacgagaaa cagatgtcgg tcggcacgtt
      841 gcagttcatg cccagcaact ggcacatcgt gaaaccctcc gttcgcggcc gctttcccgc
      901 tcaggcgccg agcttcttcc tcagcagcgc gttgacctgg gcagggccgg ccttgccctt
      961 gctcgccttc atgacctggc cgaccagggc gttgaaggcc ttctccttgc cggcgcggaa
     1021 ttcctccacc gatttctggt tggccgccag cacctcgtcg atcatggcct cgatcgcgcc
     1081 ggtgtccgtc acctgcctga ggccttgctt gtcgatgact tcgtccgccg tcgcaccctc
     1141 gccgttccac agcgcttcga acaccttcct ggcgatggcg ttggagatgg tgttgtcggc
     1201 gatgcgcgcc accaggccgg cgagctgcgc cggcgacacc ggcgaggcgg cgacgtcgag
     1261 ctcggccttg ttgaggcggg cggcgagttc gcccatgacc cagttggcgc aaggcttggc
     1321 gagcgcggcc ccggccgccg acaccgtggc ctggtagaag tcggcgactt ccttggaggc
     1381 ggtgagcgtg gtcgcgtcgt aggccgacag cccccagtcg tccatgaagc gcgccttcat
     1441 cgccccgggc agctcgggca tctccgcctg cacgcgcgcc ttccattcgg gcgaaatcat
     1501 cagcggcagc agatcggggt cggggaagta gcggtagtcg tgggcgtctt ccttgctgcg
     1561 catcatgcgc gtctcgcccg tgtccgggtc gaacagcacg gtggcctgca cgacgcgccc
     1621 gccgtcctcg agggtgtcga tctgccactg cacctcgtaa tcgatcgcct gctgcaggaa
     1681 gcggaaggag ttgaggttct tgatctcgcg ccgggtgccg aactcggtcg cgcccttcct
     1741 gcgcaccgag acgttggcgt cgcagcggaa cgagccttcc tgcatgttgc cgtcgcagat
     1801 gtcgatccag cgcaccagcg cgtgcagcgc gcgcgcgtag gccacggcct cggcggacga
     1861 gcgcatgtcg ggctcggaga cgatctcgag cagcggcgtg ccggcgcggt tgaggtcgat
     1921 gccggtcatg ccgtggaagt cctcgtggag gctcttgccg gcgtcttcct cgaggtgggc
     1981 gcgggtgaga ttgacgcgct tctcgtagtg gccgcccttg ccgtccggca ccagcaggtc
     2041 gaggtggccg ccctggacca ccggcagctc gtactggctg atctggtagc ccttggggag
     2101 gtcggggtag aagtagttct tgcg
//
LOCUS       DAOQNN010000515         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_43272_length_2122_cov_9.938075, whole genome shotgun sequence.
ACCESSION   DAOQNN010000515 DAOQNN010000000
VERSION     DAOQNN010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43272_length_2122_cov_9.938075"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1450
                     /locus_tag="PKV97_16800"
     CDS             26..1450
                     /locus_tag="PKV97_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB07595.1"
                     /translation="MPEAEKALAGIDKNRNDINSDLGKIEKLINTERGRELMRIVREK
                     EKRLAAEFDEAYKLARNDRAALAAFIRSNIAPHNNALVQALQEFSQFQGKMMEKAGRD
                     TDVMYETGRNLVVGGAIIATLLSALLGFLLARSITRPIGDALNAANALATGDLTVDIQ
                     IKGKDEVGQLMSAMHAMVGKLGEIIGEVRGAADNLANASGQVSATAQSLSQSASEQAA
                     SVEESTTSMEQMSASIVQNTENAKVTDGMASTAARQAAEGGEAVGRTVEAMKSIAEKI
                     GIVDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVRKLAERSQVAAQEIGNVAKD
                     SVKLAERAGTLLGEMVPSIKKTSDLVQEITAASQEQTAGVGQINGAMSQLNQATQQNA
                     SASEELAATAEELGGQAGQLQELMTFFRLADSGRSPTTTRRAAAAGRTPRASAPPPAA
                     PRARSGGRPAAAATPTATHDFERF"
     gene            1464..2048
                     /locus_tag="PKV97_16805"
     CDS             1464..2048
                     /locus_tag="PKV97_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNB07596.1"
                     /translation="MSLIAHNQAPAGPANALVRTGAGSVRLAAADAPQQYLTFLLGGE
                     MFAVAILNVKEIIEYGSLTEIPMMPPFIRGVINLRGAVVPVIDLAARFGGTPSRVGKR
                     SCIVILEVSEGDARHDIGVMVDAVSEVIEIPGSEIEPPPTFGARIRADFIQGMGKVSN
                     RFVIILNIVKVLSVDEIVLLAQVANSEAGGTTCN"
BASE COUNT          426 a          717 c          699 g          280 t
ORIGIN      
        1 gcgcaatgcc gcgctggccg gcaacctgcc cgaggccgag aaggcgctcg ccgggatcga
       61 caagaaccgc aacgatatca acagcgacct gggcaagatc gagaaactga tcaacaccga
      121 gcgcggtcgc gagctgatgc gcatcgtgcg cgagaaggag aagcggctcg ccgctgaatt
      181 cgacgaagcg tacaagctgg cgcgtaacga ccgggcggcg ctcgccgcgt tcatccgcag
      241 caacattgcg ccgcacaaca acgcgctggt gcaggccctg caggagttca gccagttcca
      301 gggcaaaatg atggagaagg ccggccggga taccgacgtc atgtacgaga ccgggcgcaa
      361 cctggtcgtc ggcggcgcga tcatcgccac cttgttgtcc gcgctgctcg gtttcctgct
      421 cgcgcgttcg atcacaagac cgatcggcga cgccctgaac gccgccaacg cgcttgccac
      481 gggcgatctg acggtcgaca tccagatcaa gggcaaggac gaggtcggcc aactgatgag
      541 cgcgatgcac gccatggtcg gcaagctcgg cgagatcatc ggcgaggtac gcggcgccgc
      601 cgacaacctc gccaacgctt ccggccaggt ctcggcgacc gcgcagtcgt tatcgcagtc
      661 ggccagcgag caggcggcct cggtcgagga aagcacaacg agcatggaac agatgagcgc
      721 ctcgatcgtc cagaacacgg agaatgcgaa ggtgaccgat ggcatggcct cgaccgccgc
      781 ccgccaggct gccgagggcg gcgaagcggt cggccggacg gtcgaagcga tgaagagcat
      841 cgccgaaaag atcggcatcg tcgacgacat tgcctaccag acgaacctgc tggcgctcaa
      901 cgcagcgatc gaggcggcgc gtgccggcga gcacggcaag ggcttcgcgg tcgtcgccgc
      961 cgaggtgcgc aagctggccg aacgcagcca ggtcgccgcg caggaaatcg gcaacgtcgc
     1021 caaggattcg gtcaagctgg ctgaacgcgc cggaacgctg ctcggtgaaa tggtgccctc
     1081 gatcaagaag acttccgacc tggtgcagga aatcaccgcc gcgtcgcagg aacagacagc
     1141 cggcgtcggg cagatcaacg gtgcgatgag ccagttgaac caggcgacgc agcagaacgc
     1201 ctcggcctcc gaggaactcg ccgcgaccgc cgaagaactc ggcggtcagg ccggtcagtt
     1261 gcaggaactg atgaccttct tccggctggc cgacagcggc cggtcgccaa cgaccacgcg
     1321 gcgagccgcc gcggccggtc gaacgccacg tgccagcgcg ccgccgccag cagcgccgcg
     1381 cgcccgctcc ggcgggcggc cagccgctgc agcaacgccg accgctaccc acgacttcga
     1441 acgcttctga gggagagcca acgatgagcc tgatcgccca caaccaagcg ccggccgggc
     1501 ccgccaacgc actcgtccga acaggcgccg gcagcgtccg tctggccgcc gccgacgccc
     1561 cgcagcaata cctgaccttc ctcctgggcg gcgagatgtt tgccgttgcc atcctgaacg
     1621 tgaaggaaat catcgagtac ggcagcctca ccgagatccc gatgatgccg ccgttcatcc
     1681 gcggcgtgat caacctgcgc ggcgccgtcg tgccggtgat cgacctcgcc gcgcgctttg
     1741 gcggcacgcc gagccgggtc ggcaagcgtt cgtgcatcgt catcctggaa gtcagcgagg
     1801 gcgacgcgcg acacgacatc ggcgtcatgg tcgatgccgt atccgaggtg atcgagattc
     1861 ccggcagcga gatcgaaccg ccaccgacct tcggcgccag aatccgcgcc gacttcatcc
     1921 agggaatggg caaagtgtcc aaccgcttcg tcatcatcct caacatcgtc aaggtgttgt
     1981 cggtggacga gatcgtcctc ctggcacagg tcgccaacag cgaagcgggc ggcacgacgt
     2041 gcaactagag tcggcctcgg ccaggctcgg gcgagcttgc tcaggcgcgc gctgaccggc
     2101 gaaacaggcg cctcgccgca ac
//
LOCUS       DAOQNN010000516         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_43695_length_2110_cov_8.012652, whole genome shotgun sequence.
ACCESSION   DAOQNN010000516 DAOQNN010000000
VERSION     DAOQNN010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43695_length_2110_cov_8.012652"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PKV97_16810"
     CDS             <1..841
                     /locus_tag="PKV97_16810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNB07597.1"
                     /translation="ASEGKRAEALGVELAALSGSGPGGAVTLEDVERAVAAARLPASP
                     PAATPSPISAAERQAETRRAIAAAMSRSKREIPHYYLFETVPMARAQDWLGRANAGRP
                     ITERLLMAVLQLKAVALALRKYPELNGFHRDGQFVAAQGIHVGVAISLRQGGLIAPAL
                     RDVDHKPLDVLMRGLADLVKRTRAGSLRSSEMSDPTVTVTNLGEQGVEAVAGVIHPPQ
                     VALVGFGRIAPRPWVREDGGELCVMPVVTASLAADHRVSDGHRGALFLVELRELLQRP
                     EEL"
     gene            866..1120
                     /locus_tag="PKV97_16815"
     CDS             866..1120
                     /locus_tag="PKV97_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNB07598.1"
                     /translation="MDDTELRSIVIATLKEIAPEIEEAGLRPERPLRQQVDLDSMDWL
                     NFLVGLAGKLAVEIPEADYRKLATVNDLVAYLRDARRRAG"
     gene            complement(1196..>2110)
                     /locus_tag="PKV97_16820"
     CDS             complement(1196..>2110)
                     /locus_tag="PKV97_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HNB07599.1"
                     /translation="LREAAHFALAAGAARIEREHVDAAIAAHRRRHERIRLGHLDQIL
                     RGQWLIDTAGSHVGQVNGLAVVPLGEDSFAHPQRITATVRAGAGEVIDIEREVKLGGP
                     IHSKGVLILSAFLAARFGWMLPLSLKASLVFEQSYGGVEGDSASLAELVALLSALSGV
                     AVKQSLAVTGSVNQFGVVQPVGGINEKIEGFFDLCAVRGLDGRQGVLIPRANVCHLML
                     RDDVVEAVRAGRFAVWAVADADEAVELLTGVAAGVPDEQGRMAAGSMSRRVVEGLRKL
                     ARMQREFARRGHEDDADAAGHRGRPHAS"
BASE COUNT          304 a          786 c          739 g          281 t
ORIGIN      
        1 cgcgagcgaa ggcaagcgcg ccgaggcgct gggagtggag cttgccgcgc tgtcgggcag
       61 cggcccgggc ggcgcggtca cgctggagga cgtggagcgt gccgtggcgg ctgcacgact
      121 acctgcttca ccacccgcgg cgacgccaag cccgatctcc gctgccgagc gccaggccga
      181 gacccgccgc gccatcgccg cggcgatgag ccgctcgaag cgcgagatcc cgcattacta
      241 cctgttcgaa accgtgccga tggcgcgcgc gcaggactgg ctcggccgcg ccaacgcggg
      301 acgcccgatc acagagcgcc tgctgatggc ggtgctgcag ctgaaggccg ttgccttggc
      361 gctgcgcaag taccccgagc tcaacggctt ccaccgcgac ggccagttcg tcgccgcgca
      421 gggcatccac gtcggcgtcg ccatctcgct gcgccagggc ggcctgatcg ccccggcgct
      481 gcgcgacgtg gaccacaagc cgctggatgt gctgatgcgc gggctcgccg acctggtcaa
      541 gcgcacgcgt gccggttcgt tgcgcagctc ggagatgagc gatccgacgg tcaccgtcac
      601 caatctcggc gagcagggcg tggaggcggt ggcgggcgtg atccatccgc cccaggtcgc
      661 cctcgtcggc ttcggccgca tcgcgccgcg gccctgggtg cgcgaggacg gcggcgagct
      721 gtgcgtgatg cccgtcgtca ccgccagcct cgccgccgac catcgcgtct ccgacggcca
      781 ccgcggcgcg ctgttcctcg tcgagctgcg cgagctgctg cagcggccgg aggaactgtg
      841 aactcccatt ccggacggaa ccgccatgga cgacaccgaa ctgcgcagca tcgtcatcgc
      901 aacgttgaag gaaatcgctc cggaaatcga ggaggccggc ctgcgccccg agcgcccgct
      961 gcgccagcag gtcgacctcg attcgatgga ctggctgaac ttcctggtcg gcctcgccgg
     1021 gaagctggcg gtcgagattc ccgaggcgga ctatcgcaag ctcgcgacgg tgaatgacct
     1081 ggtcgcgtac ctgcgcgacg cgcgccggcg agccggctga gcccgacgaa gatcgcccga
     1141 acggccgcaa acgtccggcc ggtgccgacc ccgctctcgg cgcgcgccac ctgcgtcagg
     1201 atgcgtgcgg ccggcctcga tgcccggccg cgtcggcgtc gtcttcgtgt ccccgccgcg
     1261 caaactcgcg ctgcatcctc gccagcttgc gcaggccctc gacgacgcgc cggctcatcg
     1321 agcccgcagc catcctgccc tgctcgtccg gcacgcccgc cgccacgccg gtgagcagct
     1381 ccaccgcctc gtcggcgtcg gccaccgccc acacggcgaa gcgcccggcc cgcaccgcct
     1441 cgaccacgtc gtcgcgcagc atcaggtggc agacgtttgc gcgcggaatc agcacgccct
     1501 ggcggccgtc gagaccgcgc accgcgcaca ggtcgaagaa gccctcgatc ttctcgttga
     1561 tcccgcccac cggctgcacg acgccgaact ggttcaccga accggtcacg gcgagcgact
     1621 gcttcaccgc cacgcccgag agcgccgaga gcagggccac cagctcggcg agcgaggcgc
     1681 tgtcgccctc gacgccaccg taggactgct cgaacaccag gctcgccttg agcgagagcg
     1741 gcagcatcca gccgaagcgt gcggcgagga aggcggacag gatcagcacg cccttggagt
     1801 ggatcggccc gccgagcttg acctcgcgct cgatgtcgat gacctcgccg gcgccggcgc
     1861 gcaccgtggc ggtgatgcgc tgcgggtggg cgaagctgtc ttcgccgagc ggcaccaccg
     1921 ccaggccatt gacctggccg acgtggctgc ccgcggtgtc gatcagccac tgcccgcgca
     1981 ggatctggtc gaggtggccg aggcggatgc gttcgtggcg gcgccggtgg gccgcgatcg
     2041 cggcgtccac atgctcgcgc tcgatgcgcg cggcgccggc ggcgagggcg aagtgggccg
     2101 cctcgcgcag
//
LOCUS       DAOQNN010000517         2097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_44218_length_2097_cov_10.823702, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000517 DAOQNN010000000
VERSION     DAOQNN010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44218_length_2097_cov_10.823702"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /gene="aroG"
                     /locus_tag="PKV97_16825"
     CDS             complement(<1..1043)
                     /gene="aroG"
                     /locus_tag="PKV97_16825"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HNB07600.1"
                     /translation="MSASIKLHIDDVRIQEIKELVPPAHVLREYPATPKAAEVAYEAR
                     QAIHRILYGADDRLLVVIGPCSIHDAKAALEYAHKLKAEAERVKDDLLIVMRVYFEKP
                     RTTVGWKGLINDPGLDNSFRINDGVRLARKLLWEVNEIGLPAGTEFLDMITPQYIADL
                     IAWGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLRIAADAIKAAQAPHHFLSVTK
                     AGHSAIVSTRGNEDCHAILRGGKEPNFDADSVEAASRELAAAGVCNKVMVDFSHGNSR
                     KQHRLQIEVAKNVAAQLAAGDDRIMGVMVESHLKEGRQDLVPGKELEYGKSVTDACIG
                     WEDSIDVLDILAEG"
     gene            1224..>2097
                     /gene="msrP"
                     /locus_tag="PKV97_16830"
     CDS             1224..>2097
                     /gene="msrP"
                     /locus_tag="PKV97_16830"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004378077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNB07601.1"
                     /translation="MLIHKPADVPPSEITPRSLFERRRDFLARSGTGLVAGAALWHGL
                     PGAARASTRLQTVPSALSTTEPLSERDAVTGYNNFYEFGTDKADPARNAHKMEVRPWT
                     VRVEGLVGKPRRFDIDELLRLAPLEERIYRLRCVEGWSMVVPWVGFPLAALLKEVEPL
                     GSAKYVEFVTHYDPKIMIRDVLDWPYTEGLRLDEALHPLTLLAVGVYGEALPKQNGAP
                     LRLVVPWKYGFKSAKSIVTIRLTEKEPATAWNKFAPQEYGFYSNVNPEVRHPRWSQAT
                     ERRIGELRKRPTQMF"
BASE COUNT          322 a          710 c          710 g          355 t
ORIGIN      
        1 ccctcggcga ggatgtcgag cacgtcgatg ctgtcttccc agccgatgca ggcgtcggtg
       61 acgctcttgc cgtactcgag ctccttgccc gggacgaggt cctggcggcc ttccttgagg
      121 tgcgactcga ccatcacgcc catgatgcgg tcgtcaccgg cggcaagctg ggcggcgacg
      181 ttcttcgcca cctcgatctg caggcggtgc tgcttgcggc tgttgccgtg ggagaagtcg
      241 accatgacct tgttgcacac tccggccgcg gcgagctcgc ggctggcggc ctcgacgctg
      301 tcggcgtcga agttgggctc cttgccgccg cgcaggatgg cgtggcaatc ctcgttgccg
      361 cgggtcgaca cgatcgccga gtgcccggcc ttggtcaccg acaggaagtg gtgcggcgcc
      421 tgcgcggcct tgatcgcgtc ggcggcgatg cgcaggttgc cgtcggtgcc gttcttgaag
      481 cctaccgggc aggacaggcc ggaggcgagc tcgcggtgca cctggctctc ggtggtgcgc
      541 gcgccgatcg cgccccaggc gatcaggtcg gcgatgtact gcggggtgat catgtcgagg
      601 aactcggtgc cggcgggcag cccgatctcg ttcacctccc acagcagctt gcgcgccaga
      661 cgcacgccgt cgttgatgcg gaagctgttg tccagcccgg gatcgttgat caggcccttc
      721 cagcccacgg tggtgcgcgg cttctcgaaa tacacccgca tcacgatcag caggtcgtcc
      781 ttgacgcgct cggcctcggc cttgagcttg tgcgcatact ccagcgccgc cttggcatcg
      841 tggattgagc acgggccgat caccaccagc agacgatcgt cggcgccgta caggatgcga
      901 tggatcgcct ggcgcgcctc gtaggcaacc tcggccgcct tcggcgtggc agggtattca
      961 cgcagcacgt gcgcgggcgg cacgagctcc ttgatctcct ggatgcggac gtcgtcgatg
     1021 tggagtttga tggaggcgga catgcgaggc tctttctcgg atctttgctg ggatgcctga
     1081 ttctagcgga aaggcgagca tggctggacg caatgcagac tataggccgg atgggatgaa
     1141 ccgatcggcg cggcgcgcgt ctgttcgtac actgccgccc gccgcgcacc accgaatcct
     1201 ccgttccaga cgagtccgca atcatgctga tccacaagcc cgccgacgtc ccgccctccg
     1261 aaatcacccc gcgcagcctg ttcgagcgcc gtcgcgactt cctcgcgcgc agcggcaccg
     1321 ggctggtggc gggcgcggcg ctgtggcacg gcctgcccgg cgccgcgcgc gcatcgaccc
     1381 ggctgcagac cgtgccctcg gcactgagca cgaccgaacc gctcagcgag cgcgacgcgg
     1441 tcaccggcta caacaacttc tacgagttcg gcaccgacaa ggccgatccg gcgcgcaacg
     1501 cccacaagat ggaggtgcgc ccgtggacgg tgcgtgtcga gggcctggtc ggcaagccgc
     1561 gccgcttcga catcgacgag ctgctgcgcc tggcaccgct ggaagaacgc atctaccgcc
     1621 tgcgctgcgt cgaaggctgg tccatggtgg tgccctgggt gggcttcccg ctcgccgcgc
     1681 tgctgaagga ggtcgagccc ctgggcagcg ccaagtacgt cgagttcgtc acccactacg
     1741 atccgaagat catgatccgc gacgtgctcg actggcccta caccgagggc ctgcgcctgg
     1801 acgaggcctt gcacccgctg accctgctcg cggtaggcgt gtacggcgag gcgctgccca
     1861 agcagaacgg cgcgccgctg cgcctggtgg tgccgtggaa atacggcttc aagagcgcca
     1921 agtcgatcgt caccatccgc ctgaccgaga aggagccggc gaccgcgtgg aacaagttcg
     1981 cgccgcagga gtacgggttc tactccaatg tgaaccccga ggtgcgccat ccgcgctgga
     2041 gccaggccac cgagcgccgc atcggcgagt tgcgcaagcg cccgacgcag atgttca
//
LOCUS       DAOQNN010000518         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_44305_length_2095_cov_9.875000, whole genome shotgun sequence.
ACCESSION   DAOQNN010000518 DAOQNN010000000
VERSION     DAOQNN010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44305_length_2095_cov_9.875000"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..633)
                     /gene="greA"
                     /locus_tag="PKV97_16835"
     CDS             complement(157..633)
                     /gene="greA"
                     /locus_tag="PKV97_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNB07602.1"
                     /translation="MNKTPLTVAGAEKLRAELHRLKTVERPNVIAAIAEARSHGDLSE
                     NAEYEAAKDRQGFIEGRIMEVESKLANAQIIDPTLLDADGRCVFGATVELEDLESGQV
                     VTYQIVGDDEADIKDNKISISSPIARALIGKYAGDIAEVQAPGGVREYEILDVRYV"
     gene            complement(630..>2095)
                     /gene="carB"
                     /locus_tag="PKV97_16840"
     CDS             complement(630..>2095)
                     /gene="carB"
                     /locus_tag="PKV97_16840"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNB07603.1"
                     /translation="TIMVNCNPETVSTDYDTSDRLYFEPITLEDLLEIVHIEKPVGVI
                     VQFGGQTPLKRAQALEENGVPIIGTSPDMIDAAEDRERFQKLLNELGLKQPPNRTART
                     PEQAVRLAAEIGYPLVVRPSYVLGGRAMEIVHEQKDLERYMREAVKVSNESPVLLDRF
                     LNDATEVDVDALSDGKQVMIGGIMEHIEQAGVHSGDSACSLPPYTLSARLQDELRRQT
                     EAMARALKVVGLMNVQFAIQGEGDNAVVYVLEVNPRASRTVPFVSKACSLPLAKIAAR
                     CMAGQSLAEQGVSGEIVPPYYSVKEAVFPFVKFPGVDTILGPEMKSTGEVMGVGRSFA
                     EAFVKSQMGAGVRLPTAGTVFISVRPTDKPVAIEVARELHEMGFALVATRGTAAAIEA
                     AGIPVGVVNKVNEGRPHIVDIIKNEEIVLVINTVDEKRQAIADSRSIRTSALAAKVSI
                     FTTIEGARAACMGMRHLSNGLEVYSVQGLHAELKQQA"
BASE COUNT          314 a          721 c          691 g          369 t
ORIGIN      
        1 cgtgcgcccg ccccgcgcgg acgcggcgcc gcacgcggcc gctgagccgc acccgcccgg
       61 ctcgcccacc cgcccggctc gccttgcgcc gaggctgcaa acaaaggggc ggtgcatcgc
      121 accgccctct tcatcgccac gccggcaagg tgcagatcag acgtagcgca cgtccaggat
      181 ctcgtactcg cgcaccccgc cgggcgcctg cacctcggcg atgtcccccg cgtattttcc
      241 gatcagcgca cgcgcgatcg gcgagctgat cgagatcttg ttgtccttga tgtcggcttc
      301 atcgtcgccg acgatctggt aggtcacgac ctggccggac tcgaggtctt cgagctcgac
      361 cgtggcgccg aacacgcagc gaccgtccgc atcgagcagt gtcggatcga tgatctgcgc
      421 gttggcgagc ttggactcga cctccatgat gcggccctcg atgaagccct ggcgatcctt
      481 ggcagcctcg tactcggcgt tctcggagag gtcgccgtgc gagcgcgcct cggcgatcgc
      541 ggcgatcacg ttggggcgct cgacggtctt cagacgatgg agttcggcac gcagcttctc
      601 ggcgccggcg acggtaagcg gggtcttgtt catgcttgct gcttcagttc ggcgtgcagg
      661 ccctgcacgg aatacacctc gagaccgttc gaaaggtggc gcatgcccat gcacgcggcg
      721 cgcgcgccct cgatggtggt gaagatcgac acctttgcgg cgagcgcgct ggtgcggatc
      781 gagcgcgagt cagcgatcgc ctggcgcttc tcatcgacgg tgttgatcac cagcacgatc
      841 tcctcgttct tgatgatgtc gacgatgtgc ggacggccct cgttgacctt gttgaccacg
      901 ccaaccggga tcccggcggc ctcgatcgcc gctgcggtgc cgcgggtggc caccagcgcg
      961 aagcccatct cgtgcagctc gcgcgcgacc tcgatcgcga ccggcttgtc ggtcggacgc
     1021 acgctgatga acaccgtgcc ggcggttggc aggcgcacgc ccgcgcccat ctggctcttc
     1081 acgaaggcct cggcgaagct gcggcccacg cccatgacct cgccggtgga cttcatctcg
     1141 ggcccgagga tggtatccac gccggggaac ttcacgaacg ggaacaccgc ctccttgacc
     1201 gagtagtaag gcggaacgat ctcaccgctg acgccctgct cggccaggct ctgacccgcc
     1261 atgcagcgcg cggcgatctt ggccagcggc agcgagcagg ccttggagac gaagggcacg
     1321 gtgcgcgagg cgcgcgggtt cacctcgagc acatagacga ccgcattgtc gccctcgccc
     1381 tggatcgcga actgcacgtt catcaggccg acgaccttca gcgcgcgcgc catggcctcg
     1441 gtctggcggc gcaactcgtc ctgcagcctc gccgacaggg tatagggtgg cagcgagcag
     1501 gccgagtcgc ccgaatgcac gccggcctgc tcgatgtgct ccatgatgcc gccgatcatc
     1561 acctgcttgc cgtcggacag cgcatcgacg tccacctccg tggcgtcgtt gaggaagcgg
     1621 tcgagcagca ccggcgactc gttcgagacc ttgaccgcct cgcgcatgta gcgctcgagg
     1681 tccttctgct cgtgcacgat ctccatcgca cgcccaccga gcacgtagga cggacgcacc
     1741 accagcggat agccgatctc ggccgcgagg cgcacggctt gttcgggcgt gcgcgcggta
     1801 cggttgggcg gctgcttgag acccagctcg ttgagcagct tctggaagcg ctcgcggtct
     1861 tcggcggcgt cgatcatgtc cgggctggtg ccgatgatcg gcacgccgtt ctcttcgagc
     1921 gcctgggcgc gcttgagcgg ggtctggccg ccgaactgca cgatcacccc caccggcttc
     1981 tcgatgtgca cgatctcgag caggtcctcg agcgtgatcg gctcgaagta caggcggtcc
     2041 gaggtgtcgt agtcggtgga cacggtctcc gggttgcagt tgaccatgat ggtct
//
LOCUS       DAOQNN010000519         2093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_44382_length_2093_cov_14.945044, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000519 DAOQNN010000000
VERSION     DAOQNN010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44382_length_2093_cov_14.945044"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..2013
                     /locus_tag="PKV97_16845"
     CDS             364..2013
                     /locus_tag="PKV97_16845"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HNB07604.1"
                     /translation="MSIRKQELIELRAQAHYWEAQHGRAKAQIEALKQELAHKDATIK
                     SLKQRLFGKKSEKHRPLPGEKDTSAREGHAPRPRGQQTGCPGHGRTPRPTLPVVAERS
                     DLPAEQRTCPQCGLPYRPTPALDEQSEVIEVQVRAYTRRITRATYTRHPGCGCDQAPA
                     VISAPPPARLIARSPFGVSFWVEVLLSKYRYGQPSNRLLHDFADQGLPVSPGTLAGGL
                     QALAGLFEPVSEALYCQQMSEPLFHNDETRWEVFVDVEGKVGTRWYLWLTRSASVVFY
                     CIDPSRSAAVPGAHFAGLQAERAIIVCDRYSAYKKLARLAENLLLAFCWAHVRRDFLE
                     AGRALPALEAWALAWKERIARLYQLNAQRLAHWDPQRPVVEQGVLFQPPQAALQAALA
                     ALQAEAERQLAQDRTAADLPKTAHTRRNQVLSSLLTHWSGLTVFVEHPAVPMDNNRAE
                     ETIRTPVIGRKNYYGSGSLWSAQLAAMLFSLFQTLALWGLNPRAWLSAYLQTCAAAGG
                     QAPRDLATFLPWSMDAARRAELSRPAARVSFAEPPAGLDSS"
BASE COUNT          356 a          709 c          686 g          342 t
ORIGIN      
        1 agcacagccc gagtgcgatc aggaaccaca ccatgaacca tcctggcaac cgtcggcacc
       61 gcgtgcgatc atggcccgtt tccgccaaca cggcgtcaat gcgcgcacgc gggatgatgc
      121 gttgcaaggc ccggagtcgc tcgaccgttg gccaagacgc gacccccgct gtttcctggt
      181 gcaggtcaat gtaaatattc ggtcacccct tctggaacgt gaagaaagta cgggacacac
      241 ccgagaaaat gttgtaaaaa gtgcgccatg gaaggtgtgg cctctctcga tctccacccc
      301 gcggcaccgc cgccgtgccg ctgcacgcct gagggcccgg tcctgccttt tgctcaggaa
      361 tgggtgagca tccgcaagca ggagctgatc gagctgagag cgcaggccca ttactgggaa
      421 gcgcagcacg gacgggcgaa ggcgcagatc gaggcgctga agcaagaact ggcgcacaag
      481 gacgccacca ttaaaagcct caagcagcgg ttgttcggca agaagagcga gaagcaccgc
      541 ccgctacccg gggagaagga caccagcgcg cgcgagggtc atgccccgcg cccgcgcggt
      601 cagcagaccg ggtgtccggg gcatggccgc accccacgcc ccaccctgcc cgtggtggcc
      661 gagcggtccg atctgcccgc tgagcagcgc acctgtccgc agtgcggctt accctatcgg
      721 cctaccccgg cgctggacga gcagagtgag gttatcgaag tgcaggtgcg cgcctatacg
      781 cggcgcatca cccgtgcgac ctacacccgc caccccggtt gtggttgtga ccaggccccg
      841 gcggtgatca gcgccccgcc gcccgcgcgg ttgatcgccc gcagtccctt cggcgtgagc
      901 ttctgggtcg aggtgctgtt aagcaagtac cgctatggcc agccgagcaa ccgcttgctg
      961 cacgacttcg ccgatcaggg cttgcccgtc tctcccggga cgctcgccgg tggcctgcag
     1021 gcgctcgccg ggctgttcga gccggtgagc gaggcgctgt actgccagca gatgagcgag
     1081 ccactcttcc acaacgacga gacgcgttgg gaagtgttcg tcgatgtcga gggcaaggtc
     1141 gggacacgct ggtatctgtg gctgacgcgc tcggcctcgg tggtgttcta ctgtatcgat
     1201 cccagccgca gcgcggcggt tcccggcgcg catttcgcgg gtctgcaggc cgagcgcgcc
     1261 atcatcgtct gcgatcgcta cagcgcctac aaaaagctcg cgcgcctggc cgagaacctg
     1321 ctgctggcct tctgctgggc gcatgtccgc cgggactttc tggaggccgg gcgcgcgctg
     1381 ccggcactgg aagcctgggc gctggcatgg aaggaacgga tcgccaggct ctaccaactt
     1441 aatgcccagc gcctggcgca ttgggacccg cagcgtccgg tcgtggagca aggcgtcttg
     1501 ttccaacccc cgcaggcggc cttgcaggcg gcgctggcgg ccttgcaggc ggaggccgaa
     1561 cggcagttgg cgcaggaccg gaccgccgcg gacttaccaa agaccgctca cacccggcgc
     1621 aaccaggtgc tcagcagtct gctcacccat tggtccgggc tgacggtctt cgtcgaacac
     1681 ccggcggtac cgatggataa caaccgcgcc gaagagacga tccgcacgcc ggtcatcggg
     1741 cgcaaaaact attatggctc gggcagcctc tggagcgcgc aactcgccgc catgttgttc
     1801 tccctcttcc agaccctggc gctgtggggc ctcaatcccc gtgcctggct cagcgcctat
     1861 ctccagacct gtgcggcggc gggtgggcag gcaccgcgtg acctggcgac ctttctgccc
     1921 tggtcgatgg atgcggcgcg gcgggctgaa ctgagccggc cagcggcgcg tgtctctttt
     1981 gccgagccgc cggccggcct cgatagttca tgagtcatcg ctattgcgga cgtgacttcg
     2041 cggccgagga gatcgccctg atccgcgcgc tcatcgccgc ggacccgacc cgc
//
LOCUS       DAOQNN010000520         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_44592_length_2088_cov_8.971471, whole genome shotgun sequence.
ACCESSION   DAOQNN010000520 DAOQNN010000000
VERSION     DAOQNN010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44592_length_2088_cov_8.971471"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PKV97_16850"
     CDS             complement(<1..215)
                     /locus_tag="PKV97_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07605.1"
                     /translation="MCRIKELTRAPGYYCATTFFDKVSAQKAVAETRATPGPDGERIM
                     KTPKLLPWYARKAGVSIERAETLWRKAV"
     gene            429..>2088
                     /locus_tag="PKV97_16855"
     CDS             429..>2088
                     /locus_tag="PKV97_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNB07606.1"
                     /translation="MSTLLSNLNPEQLQAVSLPAQHALILAGAGSGKTRVLTTRIAWL
                     IQSGQVDPAGILAVTFTNKAAKEMLARLGAMLPVSTRGMWIGTFHGLANRLLRAHHRD
                     AGLPQLFQILDSADQLAAIKRLLKTLNVDDEKFPPRELQHFINGQKEAGNRPHAVEAW
                     DDYTRLRVQLYQEYEAQCQRESVVDFAELLLRSHELLERNEPLRRHYQRRFRHILVDE
                     FQDTNRLQYRWLKLFADEGREGGAAMFCVGDDDQSIYRFRGAEVGNMRDFEREFRVNN
                     VIRLEQNYRSHANILDAANAIILHNSNRLGKNLWTDQGAGEPIRVFEAYADADEARWI
                     VEEIQALVRDGALRSEIALLYRSNAQSRVLEHQLFAAGIPYRVYGGLRFFERQEIKHA
                     LAYLRLIANPDDDTSFARVVNFPTRGIGARSLEVLTDAARTYNTSLYATVPHLSGKPG
                     SALAVFVRLVEDLRRDTAGLPLPELVDHVLDLSGLRAHYKADKEGRERLENLDELINA
                     AANFLAEAQVHSGAGEALAQPHALLADFLAHASLEAGDHQADQGA"
BASE COUNT          356 a          745 c          641 g          346 t
ORIGIN      
        1 acggccttgc gccacagggt ttcggcgcgc tcgatcgaga cgcccgcttt gcgggcatac
       61 cagggcagca gtttgggcgt tttcatgatg cgttctccgt cggggccggg tgtggccctc
      121 gtttcggcaa ccgccttctg ggcggagacc ttatcaaaaa atgttgtggc gcaatagtag
      181 ccgggcgccc gggttagttc cttgattctg cacaattccg accagtcttt ccgtgagaaa
      241 gttccgggat tccgattcgt ttggtgcagt gcacaacttc aatttagcgc aagctcggga
      301 tcctgcaaac acgttgttgc tgcgacgcaa catcatgttg tgtccttttc tctccgcgct
      361 gtccccggcc tgccgtatcg gcctcgtccc ccggggtcga tacaatgccg gctcgaagag
      421 gaaacgcgat gtccaccctg ctgagcaacc tgaaccccga acaactgcag gcggtgagcc
      481 tgcccgcgca acacgccctg atcctggccg gagccggctc gggcaagacg cgcgtgctca
      541 ccacccgcat cgcctggctg atccagtccg gccaagtcga cccggccggc atcctcgcgg
      601 ttacatttac caacaaagcc gccaaggaaa tgctcgcccg cctcggcgcg atgctgccgg
      661 tgagcacccg cggcatgtgg atcggcacct tccacggcct ggccaaccgc ctgttgcgcg
      721 cccaccaccg cgacgccggg ttgccgcagc tcttccagat cctcgactcg gctgaccagc
      781 tcgccgcgat caagcgcctg ctgaagacct tgaacgtcga cgacgagaag tttccgccgc
      841 gcgagctcca gcacttcatc aacggccaga aggaagccgg caaccgcccg cacgcggtcg
      901 aggcctggga cgactacacc cgcctgcgcg tgcagcttta ccaggagtat gaggcgcagt
      961 gccagcgcga gtctgttgtg gattttgcgg agttgctgct gcgcagccac gaactgctcg
     1021 agcgcaacga gccgctgcgc cgccactacc agcgccgctt ccgccacatc ctggtcgacg
     1081 agttccagga caccaaccgc ctgcagtacc gctggttgaa gctcttcgcc gacgagggcc
     1141 gcgagggcgg tgcggcaatg ttctgcgtcg gcgacgacga ccagagcatc taccgcttcc
     1201 gcggcgccga ggtcggcaac atgcgcgatt tcgagcgcga gttccgggtg aacaacgtga
     1261 tccgcctgga gcagaactac cgctcgcacg ccaacatcct cgacgccgcc aacgcgatca
     1321 tccttcacaa cagcaaccgc ctcggcaaga acctgtggac cgaccagggc gcgggcgagc
     1381 cgatccgtgt cttcgaggcc tatgcggatg ccgacgaggc gcgctggatc gtcgaggaga
     1441 tccaggccct ggtgcgcgac ggcgcgctgc gcagcgagat cgcgctgctc taccgctcca
     1501 acgcgcagtc gcgcgtgctc gagcaccagc tcttcgcggc cggcattccc taccgcgtgt
     1561 atggcgggct gcgcttcttc gagcgccagg agatcaagca cgcgctcgcc tacctgcgcc
     1621 tgatcgccaa cccggacgac gacacctcgt ttgcgcgcgt ggtgaacttt ccgacccgcg
     1681 gcatcggcgc gcgctcgctg gaggtgctga ccgacgccgc gcgcacgtac aacaccagcc
     1741 tctacgccac cgtgccgcat ctgtccggca agcccggcag cgcgctcgcg gttttcgtgc
     1801 gcctggtcga ggacctgcgc cgcgacaccg ccggcctgcc attgcccgaa ctggtcgacc
     1861 acgtgctcga cctgagcggc ctgcgcgcgc actacaaggc cgacaaggaa ggccgcgagc
     1921 gcctggagaa cctcgacgag ctcatcaacg ccgcggccaa cttcctcgcc gaggcccagg
     1981 tccattccgg cgccggcgag gcgctggcgc aaccgcacgc gctgctggcg gacttcctcg
     2041 cccacgcctc gctggaggcg ggcgaccacc aggccgacca gggcgcgg
//
LOCUS       DAOQNN010000521         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_44675_length_2086_cov_10.567701, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000521 DAOQNN010000000
VERSION     DAOQNN010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44675_length_2086_cov_10.567701"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /gene="tatB"
                     /locus_tag="PKV97_16860"
     CDS             complement(<1..398)
                     /gene="tatB"
                     /locus_tag="PKV97_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HNB07607.1"
                     /translation="MFDFGFSELVVIGIVMLIVVGPERLPKVARTAGHLLGRVQRYVS
                     DVKSDIQREMQLEELKKLQEQVRHQAQELETSVRTGVAGVESGIGETVAEVRSILPES
                     PASASGTAAPAPAGAPTPVARGPPPRRDLRA"
     gene            complement(495..731)
                     /gene="tatA"
                     /locus_tag="PKV97_16865"
     CDS             complement(495..731)
                     /gene="tatA"
                     /locus_tag="PKV97_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HNB07608.1"
                     /translation="MGSFSIWHWLIVLVIVLLVFGTKKLRNIGSDLGGAVKGFKEGMK
                     EGDSAGAGDASQQKLAQGQTIEGEAREKVDQNRS"
     gene            complement(742..1089)
                     /locus_tag="PKV97_16870"
     CDS             complement(742..1089)
                     /locus_tag="PKV97_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNB07609.1"
                     /translation="MSDCVFCRIVRGEIPSKKVYEDEHVFAFHDIHPAAPVHVLVVPK
                     VHVDSMAHLDGEHEAAMGRLMVAAGRIAREQGCTDGFRTIVNTGRVGLQEVYHLHLHI
                     LGGPDPLPPMLKR"
     gene            complement(1086..1424)
                     /locus_tag="PKV97_16875"
     CDS             complement(1086..1424)
                     /locus_tag="PKV97_16875"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNB07610.1"
                     /translation="MIDIEVLHRVSETLAARKKADPDSSYVSSLYQKGTDTICKKVAE
                     EAAETIMAAKDKDMLHLVWEVTDLWFHSMVLLAHHGLSVDDVLAEFRRREGVSGIDEK
                     KSRTAEGSRA"
     gene            complement(1421..1816)
                     /gene="hisI"
                     /locus_tag="PKV97_16880"
     CDS             complement(1421..1816)
                     /gene="hisI"
                     /locus_tag="PKV97_16880"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNB07611.1"
                     /translation="MSTSTRWLNEIKWDEHGLVPVIAQEAATGDVLMFAWMNREALQR
                     TAETGEAIYWSRSRRKLWHKGEESGHVQKVLDMRIDCDNDVVLLRIEQVGGIACHTGR
                     HSCFFQKYFADGRWEAVEPVLKDPQEIYK"
     gene            complement(1813..>2086)
                     /locus_tag="PKV97_16885"
     CDS             complement(1813..>2086)
                     /locus_tag="PKV97_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNB07612.1"
                     /translation="SLGAGEILLTSMDRDGTKSGFDLALTRAVSDAVRIPVIASGGVG
                     TLEHLAEGVSEGRADAVLAASIFHFGQHTVREAKELMRARGIEVRL"
BASE COUNT          338 a          724 c          663 g          361 t
ORIGIN      
        1 gctcgaaggt ctcgccgagg cggaggacct cgagcgacgg gcgtcggagc gccggccgga
       61 gcgggcgccg cggtgcccga cgcggaggca ggcgactcgg ggaggatgga gcgcacctcg
      121 gcgaccgtct cgccgattcc cgactcgacc ccggcgacgc cggtgcgcac cgaggtctcg
      181 agttcctgcg cctggtgccg cacctgttcc tgcagcttct tgagttcctc gagctgcatc
      241 tcgcgctgga tgtcggactt gacgtcggag acgtagcgtt gcacgcgccc gagcaggtgc
      301 ccggcggtgc gcgcgacctt gggcaggcgc tccggcccga ccacgatcag catcacgatg
      361 ccgatcacca cgagttccga aaagccgaaa tcgaacatgt gtgctcgctc gagacagggg
      421 ccgccaccgg cccgccgtaa aacgaagggg cgctccggtc agggcgcccc cgatgctggt
      481 tcgagacgcg aggctcagga ccggttctgg tcgaccttct cgcgcgcctc gccctcgatc
      541 gtctggccct gggcgagctt ctgctgggag gcgtcgcccg ctccggccga atcaccctcc
      601 ttcatgcctt ccttgaagcc cttcaccgca ccaccgaggt cggaaccgat gttgcgcagc
      661 ttcttggtgc cgaacaccag aaggacgatg accagcacga tcagccagtg ccagatgctg
      721 aaagaaccca taccgctctc cttaacgctt caacatgggc ggcaggggat cgggcccgcc
      781 cagaatgtgc aggtgcagat gatacacctc ctgcaggccg acccttccgg tattgacgat
      841 ggtgcggaag ccgtcggtac agccctgctc gcgggcgatc ctgcccgccg caaccatcag
      901 acgccccatc gccgcctcgt gttccccgtc gaggtgcgcc atcgagtcga cgtgcacctt
      961 gggcaccacc agcacatgca cgggtgcggc gggatggatg tcgtggaagg cgaagacgtg
     1021 ctcgtcctcg tagacctttt tcgacggaat ctcgccgcgc acgatgcggc agaagacgca
     1081 gtcgctcatg cgcgcgaacc ttccgcagtg cgcgacttct tctcgtcgat gcccgacacg
     1141 ccctcgcggc gacggaactc ggcgagcacg tcgtccacgg acagcccgtg gtgggcgagc
     1201 agcaccatgg agtggaacca gaggtcggtc acttcccaca ccaggtgcag catgtccttg
     1261 tccttggccg ccatgatggt ctcggcggcc tcctcggcga ccttcttgca gatcgtgtcg
     1321 gtgcccttct ggtacaggct ggagacgtag gaagaatccg gatcggcctt cttgcgcgcg
     1381 gcgagggtct cggacacgcg gtgcagcact tcgatatcga tcatttgtag atctcctgcg
     1441 ggtctttcaa aaccggctcg acggcctccc agcggccatc ggcaaaatac ttctggaaga
     1501 agcagctgtg acgtccggtg tggcaggcga tcccgcccac ctgctcgatc ctgagcagga
     1561 ccacgtcgtt gtcgcagtcg atgcgcatgt ccagcacctt ctgcacgtgg ccggactcct
     1621 cgcccttgtg ccacagcttg cgccgcgaac gcgaccagta gatggcttcg ccggtctcgg
     1681 ccgtgcgctg cagcgcctcg cggttcatcc aggcgaacat cagcacgtcg ccggtggcgg
     1741 cttcctgcgc gatgaccggc accaggccgt gctcgtccca cttgatttcg ttcaaccagc
     1801 gcgtgctcgt gctcacaagc gtacctcgat gccgcgcgcg cgcatcaact ccttggcctc
     1861 gcgcacggtg tgctgtccga aatggaagat gctcgccgcc agcaccgcgt cggcgcggcc
     1921 ttcggagacc ccctcggcga gatgctcgag cgtgccgacg ccaccgctgg cgatcaccgg
     1981 gatgcgcacc gcatccgaca ccgcgcgcgt gagcgcgagg tcgaagccgc tcttggtgcc
     2041 gtcgcggtcc atgctggtga gcaggatctc gcccgccccc agcgac
//
LOCUS       DAOQNN010000522         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_44677_length_2086_cov_7.674545, whole genome shotgun sequence.
ACCESSION   DAOQNN010000522 DAOQNN010000000
VERSION     DAOQNN010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44677_length_2086_cov_7.674545"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PKV97_16890"
     CDS             complement(<1..776)
                     /locus_tag="PKV97_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNB07613.1"
                     /translation="MNSIRRRLSLTLALVLLAAGVLLAFALRDFPRRMVEEHVLARLD
                     HDADLLYVHLLDALARQDGEAGMDSALMGAAGPAYDLPLSGHYFLVRLGDRQWRSRST
                     WDADLDLPEGAEGLVSPRLAGPAGQALLVVAKRFPDSADGRAVSIALAEDVAAIDAAI
                     AAFRAQALTVLGLALLVLLVLQRRVLVRGLAPLDEAVDACRRLERGEAVQFEADAPAE
                     VRPLLAAVERLSRHQAQRLGRIRHAAGNLSHALKTPLAVLG"
     gene            complement(804..1469)
                     /locus_tag="PKV97_16895"
     CDS             complement(804..1469)
                     /locus_tag="PKV97_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB07614.1"
                     /translation="MRLLVVEDDAALGERLRTRLVAEGYAVDLARDGIDGAHLGATEP
                     YDAVILDLGLPGKPGLTVLREWRAAANRVPVLILTARDGWAERVEGLQAGADDYLGKP
                     FHVEELLARIQALLRRAAPVPDTELRAGDWRLDEARQCLVDRAGNEQALTATEFRLLR
                     YFMRHPDEVLSKTRLSEHVYDYDAERDSNVIEVYVRRLRGLLGNERIETRRGQGYVFR
                     SGD"
     gene            complement(1473..1793)
                     /locus_tag="PKV97_16900"
     CDS             complement(1473..1793)
                     /locus_tag="PKV97_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNB07615.1"
                     /translation="MKTRFRSLLAAAALLVAGGLSVSGASAGERVRVEEIRQLRESGQ
                     ILSAEDILARSRAIQPGQVVGLELEREDGRWIYEVKLIDERNRVHELELDAANGEVLE
                     RKEK"
BASE COUNT          302 a          780 c          739 g          265 t
ORIGIN      
        1 ccgagcaccg cgagcggcgt cttgagggcg tgggagaggt tgcccgcggc gtggcggatg
       61 cgcccgaggc gctgggcctg gtggcgcgac aggcgctcga cggcggcgag cagcgggcgc
      121 acctcggcgg gcgcgtcggc ctcgaactgc acagcctcgc cgcgctccag ccgccggcag
      181 gcgtccaccg cctcgtccag cggtgccagc ccgcgcacca gcacgcgccg ctgcagcacc
      241 agcagcacga gcagcgccag tccgagcacg gtcagcgcct gcgcgcggaa ggccgcgatc
      301 gcggcatcga tcgcggcgac gtcctcggcg agcgcgatgc tcaccgcgcg gccgtcggcg
      361 ctgtcgggga agcgcttcgc gaccaccagc agcgcctgcc cggcagggcc ggccaggcga
      421 ggcgacacaa gcccctccgc cccctccggc aggtcgaggt cggcgtccca ggtcgagcgc
      481 gaacgccact gccgatcgcc gaggcgcacg aggaagtaat gccccgacag cggcaggtcg
      541 taggccggcc cggccgcgcc catcagcgcg ctgtccatcc ctgcctcgcc gtcctggcgg
      601 gcgagcgcgt cgagcagatg cacgtagagc agatcggcgt cgtgatcgag gcgggcgagc
      661 acgtgctcct cgaccatgcg gcgcggaaag tcgcgcagtg cgaaggccag cagcacgccc
      721 gcggcgagca gcaccagggc gagcgtgagc gagaggcggc ggcggatcga gttcatcttg
      781 cggccggccc ccaggcatgc gcctcagtcg ccgctgcgga acacatagcc ttgcccgcgc
      841 cgggtctcga tgcgctcgtt gccgagcagg ccgcgcaggc ggcggacgta gacctcgatg
      901 acgttgctgt cgcgctcggc gtcgtagtca tagacgtgct cggagagccg cgtcttcgac
      961 agcacctcgt cggggtggcg catgaaatag cgcagcaggc ggaactcggt ggcggtcagg
     1021 gcctgctcgt tgccggcgcg atcgaccagg cactggcgcg cctcgtccag ccgccagtcg
     1081 cccgcgcgca actcggtgtc gggcaccggc gcggcgcggc gcagcagggc ctggatgcgg
     1141 gcgaggagtt cctcgacgtg gaagggcttg cccagataat cgtcggcgcc ggcctgcagg
     1201 ccctcgacac gctcggccca gccgtcgcgc gcggtgagga tcagcaccgg cacgcggttc
     1261 gccgccgcgc gccactcgcg cagcacggtc agccccggct tgccgggcag gccgaggtcg
     1321 aggatcaccg cgtcgtaggg ctcggtcgcg cccagatggg cgccgtcgat gccgtcgcgc
     1381 gcgaggtcca ccgcatagcc ttcggcgacc agccgcgtgc gcaggcgctc gccgagcgcc
     1441 gcatcgtcct cgaccacgag caggcgcatc gctcacttct ccttgcgttc gagcacttcc
     1501 ccgttggcgg cgtcgagctc gagttcgtgc acccggttgc gctcgtcgat cagcttgacc
     1561 tcgtagatcc agcgtccgtc ctcgcgctcg agctccagac cgacgacctg gccaggctgg
     1621 atggcgcggc tgcgcgcgag aatgtcctcg gcggagagga tctgcccgct ctcgcgcagc
     1681 tggcggatct cctccacgcg cacgcgctcg cccgcgctcg cgccggacac ggacaggcca
     1741 cctgcgacaa ggagcgcggc ggcggccagc agggaacgga atcgcgtctt catcttcatt
     1801 ctccggcggg tttcgacgca gacagtgtgc ccgccgcaat ctgaatgcaa gctgaacacg
     1861 agcacgcaga tcgcgactcg cgtcgctccc acaagaacca ccgcggctcc gcagcgcgcc
     1921 gtaggagcga cgcaagtcgc gattcccccg tccggccctc gccgccgtcg gtcgccgccc
     1981 agcggcccga tcgcgactgg cgtcgctccc acaagaacca ccgcggctcc gcagcgcgcc
     2041 gtaggagcga cgcaagtcgc gattcccccg tccggccctc gccgcc
//
LOCUS       DAOQNN010000523         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_44954_length_2079_cov_12.254447, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000523 DAOQNN010000000
VERSION     DAOQNN010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44954_length_2079_cov_12.254447"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2079)
                     /locus_tag="PKV97_16905"
     CDS             complement(<1..>2079)
                     /locus_tag="PKV97_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HNB07616.1"
                     /translation="ACRKVGLGKDVTDKFLGGVPGVQKEGTDGLIVAARWVLHKMPPV
                     TRTVCLEFFGQVREAVPAIVEITDFFKPGGAGHAAGVQLAGLEHLDERYVKAVGYATK
                     ARRHGRPKMVLIGDIVGHDEDAVMKAASEVVRMTNARGSEGFIAVSPEQRKRFWLERS
                     RTAAISRHTNAFKINEDVVIPLPRMGEYCDGIERINIELSLQNKIALCDRLAEFLQGE
                     LPLEIKDEGLDREALVSDHRAQALEQVAGVRRRWQWLLDKLDLALAEAEPQFAAYGVQ
                     AGELSNRAASPTLFHRLQDYSIRVSWKEELRPLLEQTFDGALFAPILARIAEIHREVL
                     RGRVFVALHMHAGDGNVHTNLPVNSDHYEMLQTANRAVDRIMALARSLDGVISGEHGI
                     GITKLDYLTDDEMANFWSYKEKVDPEGRFNRGKLMRKGPIRGNLDNAYTPSFNLMGHE
                     SLIMEQTAIGEISHDIKDCLRCGKCKPVCSTHVPRANLLYSPRNKILSTSLLIEAFLY
                     EEQTRRGVSLAHWAEFEDVADHCTICHKCEKPCPVDIDFGDVSIKMRNLLRKQGKKSF
                     NPGKAAAMAFLTIKDPTTIKAIRTGMIDWGYKAQRFAHELGKKFGLVQPQVKAPPATL
                     GKAPIKAQVIHFINKPMPGNLPKRTSRALLDIEDDKVIPVIRDPHKANGESEAVFYFP
                     GCGSERLFSQ"
BASE COUNT          290 a          658 c          754 g          377 t
ORIGIN      
        1 cctggctgaa caggcgctcg gagccgcagc ccgggaagta gaacaccgcc tcggactcgc
       61 cgttggcctt gtgcgggtcg cggatcaccg ggatgacctt gtcgtcctcg atgtcgagca
      121 gcgcgcggct ggtgcgcttg ggcaggttgc cgggcatcgg cttgttgatg aagtggatca
      181 cctgggcctt gatcggggcc ttgccgagcg tggccggggg cgccttcacc tgcggctgga
      241 cgaggccgaa cttcttgccc agctcgtggg cgaagcgctg cgccttgtag ccccagtcga
      301 tcatgccggt gcggatggcc ttgatcgtgg tcgggtcctt gatggtcagg aaggccatcg
      361 ccgccgcctt gcccggattg aacgacttct tgccctgctt gcgcagcagg ttgcgcatct
      421 tgatcgacac gtcgccgaag tcgatgtcca ccggacaggg cttctcgcac ttgtggcaga
      481 tcgtgcagtg gtcggccacg tcctcgaact cggcccagtg cgccagcgac acgccgcgcc
      541 gggtctgctc ctcgtacagg aaggcctcga tcaacagcga ggtggagagg atcttgttgc
      601 gcggggaata cagcaggttg gcgcgcggca cgtgcgtgga gcacaccggc ttgcacttgc
      661 cgcagcgcag gcagtccttg atgtcgtggg agatctcgcc gatggcggtc tgctccatga
      721 tcagcgactc gtggcccatc aggttgaagc tgggggtgta ggcgttgtcg aggttgccgc
      781 ggatcggccc cttgcgcatc agcttgccgc ggttgaagcg gccttcgggg tcgacctttt
      841 ccttgtagct ccagaagttc gccatctcgt cgtcggtgag atagtcgagc ttggtgatac
      901 cgatgccgtg ctcgccggag atgaccccgt ccaggctgcg tgccagcgcc atgatgcggt
      961 cgaccgcgcg gttggcggtc tgcagcatct cgtagtggtc ggagttgacc ggcaggttgg
     1021 tgtgcacgtt gccgtcgccg gcgtgcatgt gcagggcgac gaagacgcgg ccgcgcagca
     1081 cctcgcggtg gatctcggcg atgcgggcga ggatgggggc gaacagggcc ccgtcgaagg
     1141 tctgctcgag cagcgggcgc agctcctcct tccacgacac gcggatcgag tagtcctgca
     1201 ggcgatggaa cagggtcggg ctggcggcgc ggttggagag ctcgccggcc tggacgccat
     1261 aggcggcgaa ctgcggctcg gcctcggcga gcgcgaggtc gagcttgtcc agcagccact
     1321 gccagcgccg gcgcacgccg gcgacctgct cgagggcctg ggcgcggtgg tcggagacca
     1381 gcgcctcgcg gtcgaggccc tcgtccttga tctcgagcgg cagctcgccc tgcaggaact
     1441 cggcgaggcg gtcgcagagc gcgatcttgt tctgcagcga gagctcgatg ttgatgcgct
     1501 cgatgccgtc gcagtattcg cccatgcgcg gcagcgggat gaccacgtcc tcgttgatct
     1561 tgaaggcgtt ggtgtggcgc gagatcgccg cggtgcggct gcgctcgagc cagaagcgct
     1621 tgcgctgctc ggggctgacc gcgatgaagc cctccgaacc gcgcgcgttg gtcatgcgca
     1681 cgacctcgga ggcggccttc atcaccgcgt cctcgtcgtg gccgacgatg tcgccgatca
     1741 gcaccatctt ggggcggccg tggcggcgcg ccttggtggc gtagccgacc gccttgacgt
     1801 agcgctcgtc gaggtgctcg aggccggcga gctgcacgcc cgcggcgtgg ccggcgccgc
     1861 ccggcttgaa gaaatcggtg atctcgacga tcgccggcac cgcctcgcgc acctggccga
     1921 agaactccag gcacaccgtg cgggtgaccg gcggcatctt gtgcagcacc cagcgtgcgg
     1981 ccacgatgag gccgtcggtg ccttccttct gcacgcccgg cacgccgccg aggaacttgt
     2041 cggtgacgtc cttgccgagg ccgaccttgc ggcaggcgg
//
LOCUS       DAOQNN010000524         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_45142_length_2075_cov_9.705941, whole genome shotgun sequence.
ACCESSION   DAOQNN010000524 DAOQNN010000000
VERSION     DAOQNN010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45142_length_2075_cov_9.705941"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..>2075)
                     /locus_tag="PKV97_16910"
     CDS             complement(41..>2075)
                     /locus_tag="PKV97_16910"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB07617.1"
                     /translation="KFYVGDVDGNPGKSLVVELDGPRRGLWKDFSTDEGGDIIDLWAR
                     SQGRSARSDFPRIAGEIRQWLGIAAPVGTPMRRDVRSMPMDDLGAYTGKWDYLTPDGE
                     LIACVYRYDPPTGKEYRPWDVRARMWRAPDPRPLYNQPAIAKAREVVLVEGEKCAAAL
                     VACGIAATTAMNGAKAPVDKTDWRPLAGKSVVIWPDRDAPGWDYAESAARACVMAGSA
                     SVAILVPPTDKPAKWDAADAVDEGFDCAAFIAQGDRRIVKAAASSLPTFTLGELLDDN
                     SPLPPDLIAPRVLTPAGMLVFGGAPKVGKSDFLLSWLAHMAAGAVFLGMQPPRPLRVF
                     YLQAEVQYHYLRERVKDVRLPSHRLLDARANFVATPQLRLVLDDAGLAQVIPAIAQAF
                     GGEPPDIIAIDPIRNVFDGGDAGGENDNGAMLFFLSQRVERIRQAVNPDAGVILAHHT
                     KKLGKKQFEEDPFQALAGAGSLRGYYSTGMLLFRPDETRTTRQLIFELRNGAGIPQRH
                     VDKINGEWREVDANERLVMKDYGERLDAERRRKRDAILQILFEEAGNGRCYTANQFAE
                     SFEGKAGLGGERTIRERVSALSTQGYIKYFRNAADYGLPSSGRTKFGYLCVEGMVLRT
                     QAGEVDTATGELPMREYTVLPTHYKCPHSGASMPVENPDVWVYHDELNDPEAS"
BASE COUNT          346 a          671 c          686 g          372 t
ORIGIN      
        1 cagttggcaa cggcgctgcc aactgccgat tgggcaatgt tcatgaggcc tccgggtcgt
       61 tcagttcgtc gtggtagacc cacacgtcgg ggttctcgac aggcatcgag gcgccggaat
      121 gcgggcactt gtagtgggtt gggagcaccg tgtactcgcg catcggcaac tcgccggtgg
      181 ccgtgtcgac ttcgccagct tgcgtacgca gcaccatgcc ttcgacgcag agatagccga
      241 acttagtgcg gccgctggag ggcagaccgt agtcagccgc gttgcggaaa tacttgatgt
      301 agccctgcgt cgacagcgcg gagacgcgtt cgcggatggt gcgctcgccg ccgagaccgg
      361 ccttgccctc gaaggactcc gcgaactggt tggcggtgta gcagcgcccg ttgccggcct
      421 cctcgaacaa gatctggagg atcgcgtcgc gcttgcggcg gcgctcggca tccaagcgct
      481 cgccgtagtc cttcatcacc agccgctcgt tggcatcgac ctcgcgccac tcgccgttga
      541 tcttgtcgac atgccgttgc gggatacccg cgccgttgcg cagctcaaag atcagctggc
      601 gggtcgttct ggtctcgtcg ggcctgaaca acaacatccc ggtcgagtag tagccgcgca
      661 gacttcccgc gccggccagt gcctggaacg ggtcctcctc gaactgcttc ttgccgagct
      721 tcttggtgtg gtgggcgagg atgacgccgg cgtccggatt cactgcctgg cgaatgcgct
      781 ccacccgctg ggacaggaag aacagcatcg cgccgttgtc gttctcgcca ccggcgtcac
      841 cgccgtcgaa aacgttgcgg atcggatcga tggcgatgat gtcgggaggc tcgccgccga
      901 aagcctgcgc gatcgccggg atcacctgcg ccagccctgc gtcatcgagc accagccgca
      961 actgcggtgt ggcgacgaag ttggcgcggg catccaaaag ccggtgtgat ggcaggcgca
     1021 catccttcac gcgctcgcgc aggtagtggt actggacctc ggcctgcagg tagaacacgc
     1081 gcagcggacg gggtggctgc atgccgagga atacagcgcc tgcagccatg tgcgccagcc
     1141 aagacagcag gaagtcgctc ttgccgactt tcggtgcgcc accgaacacc aacatgccag
     1201 ccggtgtcag cacgcgcgga gcgatcagat ccgggggcag cggcgagttg tcatcgagca
     1261 gttcgccgag cgtgaaggtg ggcagagagg aagccgctgc ctttacgatc cgtcggtcac
     1321 cctgggcgat gaatgccgca cagtcgaagc cctcgtcgac agcgtctgct gcatcccact
     1381 tggccggctt gtcggtcggc ggcaccaaaa tggccacgga tgcgctgccc gccatcacgc
     1441 aagcgcgcgc tgcgctctcg gcgtagtccc agccgggggc atcccggtcc ggccagatga
     1501 ccacggattt cccggctaat ggacgccagt cggtcttgtc gactggtgcc ttggcgccgt
     1561 tcatcgccgt ggtggccgca atgccgcaag caaccaatgc agccgcacat ttctcgcctt
     1621 cgaccaggac gacctctcgc gctttcgcga tggccggttg gttgtagagc ggcctggggt
     1681 cgggggcgcg ccacatgcgg gcgcggacat cccaggggcg gtactctttg cctgtcggcg
     1741 ggtcataccg gtagacgcag gcgatcagct cgccatcggg agtcaggtaa tcccatttgc
     1801 cggtgtaggc gccgaggtcg tccatcggca tgctgcgaac atcacggcgc atcggcgtgc
     1861 cgaccggggc agcaatgccg agccattgcc ggatctctcc agcgatgcgt gggaagtcgc
     1921 tgcgggcgga gcggccctgc gagcgcgccc acagatcaat gatgtcgccg ccctcgtcgg
     1981 tggagaagtc tttccacagg cctcgccgtg gtccgtccag ctcaaccacc agactcttgc
     2041 ccgggttgcc atcgacatca ccgacgtaga acttg
//
LOCUS       DAOQNN010000525         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_45355_length_2070_cov_9.920099, whole genome shotgun sequence.
ACCESSION   DAOQNN010000525 DAOQNN010000000
VERSION     DAOQNN010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45355_length_2070_cov_9.920099"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PKV97_16915"
     CDS             <1..513
                     /locus_tag="PKV97_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNB07618.1"
                     /translation="AAFIGRARVVPLRNTGAAISPFNSFLILQGIETLALRMDRICTN
                     TIKVAEYLKKHAKVEWVNYAGLPDHADHALVQKYMGGRASGILSFGVKGGFEAGGRFQ
                     DALKLITRLVNIGDAKSLACHPASTTHRQLSPAELAKAGVSPDMVRLSIGIEHIDDIV
                     ADLEQALAAV"
     gene            666..1139
                     /gene="dtd"
                     /locus_tag="PKV97_16920"
     CDS             666..1139
                     /gene="dtd"
                     /locus_tag="PKV97_16920"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNB07619.1"
                     /translation="MLAVLQRVSEARVIVDGETIGEIGPGLLALVCAERGDTPAEADK
                     LLARILKLRIFADEAGKMNRSVQDVGGGLLVVSQFTLAADTSGGNRPSFTKAADPHTG
                     QALYEHFVARARAAHAQVETGRFGADMKVQLINDGPVTVPLRIAPTAADDPRGFA"
     gene            1312..1917
                     /gene="gstA"
                     /locus_tag="PKV97_16925"
     CDS             1312..1917
                     /gene="gstA"
                     /locus_tag="PKV97_16925"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HNB07620.1"
                     /translation="MKLYFKPGACSLSPHIALEESGLPYETESVDLKTKVTASGADFW
                     KSNPKGYVPALLLDSGELLTEGAAIVQYIADQAPQKKLAPANGTIERYRLQSWLNFIG
                     TELHKSCSPFFNPASGKEWKEIAGANLERRLGYVNEQIDGKSYLLGEDFSVADGYLFT
                     VLSWMKFVGIDLAKWPNLSAFQARVAARPAVQAALKAEGLA"
BASE COUNT          350 a          747 c          648 g          325 t
ORIGIN      
        1 gccgccttca tcggccgcgc gcgcgtggtg ccgctgcgca acaccggcgc ggcgatctcg
       61 cccttcaaca gcttcctgat cctgcagggc atcgagaccc tggcgctgcg catggaccgc
      121 atctgcacca acacgatcaa ggtcgccgag tacctgaaga agcacgccaa ggtcgagtgg
      181 gtgaactacg ccggcctgcc cgaccacgcc gaccacgccc tggtgcagaa gtacatgggc
      241 gggcgcgcct cgggcatcct gtccttcggg gtcaagggcg gctttgaagc cggcggccgc
      301 ttccaggatg cgctgaagct catcacccgc ctggtgaaca tcggcgacgc caagtcgctc
      361 gcctgccacc cggcctccac cacccaccgc cagctctcgc cggccgaact cgccaaggcc
      421 ggcgtgtcgc ccgacatggt gcgactgtcg atcggcatcg agcacatcga cgacatcgtt
      481 gccgatctgg agcaggcgct ggcggcggtc tgacgtagtt cttgatgcgt ctgtccggga
      541 cagaccgcgc atttcctgcg aaatcgtggt ctgtccccgt ttccttccgt ggtttgtccc
      601 tgcttccttt cttccccgtt tcccgcggct ttccgcacag accacggctt tcacgagaaa
      661 ccccgatgct cgcagtcctg cagcgcgtct ccgaagcgcg tgtgatcgtc gatggcgaga
      721 ccatcggcga gatcggcccc ggcctcctcg cgctggtgtg cgccgagcgc ggcgacacgc
      781 ccgccgaggc cgacaagctg ctcgccagga tcctcaagct gcgcatcttc gccgacgaag
      841 ccgggaagat gaaccgctcg gtgcaggacg tcggaggcgg gctgctggtg gtgagccagt
      901 tcaccctcgc cgccgacacc tcgggcggca accggccgag cttcaccaag gccgcagacc
      961 cgcacaccgg ccaggcgctc tacgagcatt tcgtcgcccg tgcccgcgcc gcccacgcgc
     1021 aggtggagac cggccgcttc ggcgccgaca tgaaggtgca actcatcaac gacggcccgg
     1081 tcacagtgcc gctgcgcatc gccccgaccg cagcggacga cccccgcggt tttgcgtagg
     1141 agcgacgcga gtcgcgatcg gcctcgctgc tgcgaaggac gccggcggga gtccgaacgc
     1201 tgcaatcgcg acttgcgtcg ctcctacccc ccgccccacc ggcggcgcgc gactgtttca
     1261 tggcgcgtcg gggcataatc gtggctccca acccctcgac caggagacaa catgaagctc
     1321 tacttcaaac ccggtgcctg ctcgctctcg ccccacatcg cgctggaaga atccggcctg
     1381 ccctacgaga ccgagtcggt cgacctcaag accaaggtga ccgccagcgg cgccgacttc
     1441 tggaaaagca accccaaggg ctacgtcccg gcgctgctgc tcgacagcgg cgagctgctc
     1501 accgaaggcg cggcgatcgt gcagtacatc gccgaccagg cgccgcagaa gaagctcgcg
     1561 ccggccaacg gcacgatcga gcgctaccgc ctgcagtcat ggctcaactt catcggcacc
     1621 gagctgcaca agtcctgcag ccccttcttc aacccggcct cgggcaagga gtggaaggag
     1681 atcgccggcg ccaacctcga gcgccgcctc ggctatgtaa acgagcagat cgatggcaag
     1741 tcctacctgc tgggcgagga cttcagcgtc gccgacggct atctgttcac cgtgctgagc
     1801 tggatgaagt tcgtcggcat cgacctggcg aagtggccca acctgagcgc ctttcaggcc
     1861 cgcgtggcgg cgcgtccggc ggtacaggcg gcgctgaagg cggaaggcct ggcctgagtg
     1921 gcgcgaagca tcgccagcag atcgtgaatg cgcggctgcc ggcgctcctg caggggccga
     1981 cgccgcgatg tctctgcagg agcgccgcaa ggcgctagga ccgccatcgt tctctcgcat
     2041 cggctcgatg atcgccaccg tcacagctag
//
LOCUS       DAOQNN010000526         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_45760_length_2060_cov_12.930673, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000526 DAOQNN010000000
VERSION     DAOQNN010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45760_length_2060_cov_12.930673"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2060
                     /gene="rpoB"
                     /locus_tag="PKV97_16930"
     CDS             <1..>2060
                     /gene="rpoB"
                     /locus_tag="PKV97_16930"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNB07621.1"
                     /translation="RIDRRRKLPATTLLLALWNKGVEQKLAKGGELSDEDRRFSGMSA
                     EEILNYFYSQVVFSKSKKGWTAPFEPERMKGVKLTAELVDAKTGKAVAEIGAKMTPRL
                     GKKLQEAGLKEILVQPEELHGRYAAVDIVNEKTGEIFVEAGAEITQAVLDQLESAGVK
                     ELPTLGIDHVNVGPYMRNTLMADRNSSREEALIDIYRVMRPGEPPTLDTAEALFQGLF
                     FDLERYDLSSVGRVKMNSRLNLNSPDSIRVLRKEDVLAILKVLVDLKDGRGEIDDIDH
                     LGNRRVRSVGELLENQYRLGLLRMERAIRERMSSVEIDTVMPHDLINAKPAAAAVREF
                     FGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRICPIET
                     PEGPNIGLINSLATFARVNQYGFIESPYRKVEKGTVTDEVIYLSAMEEGKYTIAQANA
                     RLDTRNKLADDLISCRRAGEFVMARREDVQFIDVSPKQLVSVAAALIPFLENDDANRA
                     LMGSNMQRQAVPLLQADAPLVGTGMEGTVARDSGVAIAARRKGIVDQVDATRIVIRAT
                     EETNTAAPGVDIYNLLKFQRSNQNTCINQRPLVKVGDVVEKGEIIGDGPSTDLGELAL
                     GRNVLCAFMPWNGYNFEDSILISERIVKDDVFTSIHIEEFEVMARDTKLGQEEITRDI
                     PNVG"
BASE COUNT          391 a          696 c          675 g          298 t
ORIGIN      
        1 cgcatcgacc gccgccgcaa gctgccggcg acgacgctgc tgctcgccct gtggaacaag
       61 ggcgtcgagc agaagctggc caagggcggc gagctctccg acgaggaccg ccgcttcagc
      121 ggcatgtcgg cggaagagat cctgaactac ttctacagcc aggtcgtctt ctccaagagc
      181 aagaagggtt ggaccgcgcc gttcgagccg gagcggatga agggcgtgaa gctgacggcc
      241 gagctggtcg acgcgaagac cggcaaggcg gtggccgaga tcggcgccaa gatgactccg
      301 cgcctgggca agaagctgca ggaggcgggc ctcaaggaaa tcctggtcca gccggaagag
      361 ctgcacggcc gctatgccgc cgtcgacatc gtcaacgaga agaccggcga gatcttcgtc
      421 gaggccggcg ccgagatcac ccaggcggtg ctcgaccagc tcgagtccgc cggcgtcaag
      481 gagctgccga cgctcggcat cgaccacgtc aatgtcggcc cctacatgcg caacacgctg
      541 atggccgaca ggaactcctc gcgcgaggaa gcgctgatcg acatctaccg cgtcatgcgc
      601 ccgggcgagc cgccgacact ggacaccgcg gaagcgctgt tccagggtct tttcttcgac
      661 ctcgagcgtt acgatctgtc atcggtcggc cgcgtgaaga tgaactcgcg gctcaacctc
      721 aactcgccgg attcgatccg cgtgctccgc aaggaggacg tgctcgccat cctcaaggtc
      781 ctcgtcgacc ttaaggacgg ccgcggcgag atcgacgaca tcgaccatct cggcaaccgc
      841 cgcgtccgct cggtgggcga gctgctggag aaccagtacc gcctcggcct gctgcgcatg
      901 gagcgcgcga tccgcgagcg catgagctcg gtcgagatcg acacggtcat gccgcacgac
      961 ctgatcaacg ccaagccggc ggcggccgcg gtgcgcgagt tcttcggctc gtcgcagttg
     1021 tcgcagttca tggaccagac caacccgctg tcggagatca cccacaagcg gcgcctctcg
     1081 gcccttggcc cgggcggcct gacccgcgag cgcgccggct tcgaggtgcg cgacgtgcac
     1141 ccgacgcatt acggccgcat ctgcccgatc gagacgccgg aaggcccgaa tatcggcctg
     1201 atcaactcgc tcgccacctt cgcgcgggtg aaccagtacg gcttcatcga gagcccgtac
     1261 cgcaaggtcg agaagggcac cgtcaccgac gaggtgattt acctgtcggc gatggaagag
     1321 ggcaaataca ccatcgccca ggccaatgcg cgcctcgaca cgcgcaacaa gctggccgat
     1381 gacctgatct cctgccgccg cgccggcgag ttcgtgatgg cgcgccggga ggacgtgcag
     1441 ttcatcgacg tcagccccaa gcagctggtc tcggtggccg cggcgctgat cccgttcctc
     1501 gagaacgacg acgccaaccg cgcgctgatg ggctcgaaca tgcagcgcca ggcggtgccg
     1561 ctgctccagg ccgatgcgcc gctggtcggc accggcatgg agggcacggt ggcccgcgac
     1621 agcggtgtcg ccatcgccgc ccgccgcaag ggcatcgtcg accaggtgga cgccacccgc
     1681 atcgtcatcc gcgcgacgga ggagacgaac acggcggcgc cgggcgtcga catctacaac
     1741 ctgctgaagt tccagcgctc caaccagaac acctgcatca accagcgtcc gctggtgaag
     1801 gtgggcgacg tggtggagaa gggcgagatc atcggcgacg gtccctcgac cgacctcggc
     1861 gagctggcgc tgggccgcaa cgtgctctgc gccttcatgc cgtggaacgg ctacaacttc
     1921 gaggactcga tcctgatctc cgagcggatc gtgaaggacg acgtcttcac ctcgatccac
     1981 atcgaggaat tcgaggtcat ggcccgcgac accaagctgg gccaggagga aatcacccgc
     2041 gacatcccga acgtgggtga
//
LOCUS       DAOQNN010000527         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_45955_length_2055_cov_9.656500, whole genome shotgun sequence.
ACCESSION   DAOQNN010000527 DAOQNN010000000
VERSION     DAOQNN010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45955_length_2055_cov_9.656500"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1563)
                     /locus_tag="PKV97_16935"
     CDS             complement(145..1563)
                     /locus_tag="PKV97_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07622.1"
                     /translation="MLKPPVYSHLVDAAGTYGPEVAELAAMAGLPPDPEQRLCLDQMF
                     AVTPSGTPAAFETAIVAPRQNLKTGTLKQGALGWMFLLDRRLIVWSAHEFSTAQEAFR
                     DMLELVEGTPELDRKVKAVHRANGDEAIELLNGCRLKFKARTKAGGRGLTGDVVILDE
                     AFALRPAQMGALLPTLTTRPMAQVVYASSAGGLDAAVLRGIRDSGRAGNKPRLAYLEW
                     CDPDPPACAVDGCEHGYGTAGCSLDDRGRWRRAMPALGRRITEDIMLAFRQSGMPPDE
                     FSREFLGWWEDPADSTDDLTGAEWAAAETEDGPTGDLVAAADVAPNGVWSSVVVCGAG
                     ILELVEHRRGTSWLPERLAELRDRHRIAEIGIDPAGPVGSLLPDLERAGVPVRLLDGR
                     DTVRACGAMVTAVADAAVRHRGEPALMAAVGGASRRTVGDGWKWSRKDSTVDVSPLVA
                     ATYAHWMWLGRAVAYDILDSAW"
     gene            complement(1556..1795)
                     /locus_tag="PKV97_16940"
     CDS             complement(1556..1795)
                     /locus_tag="PKV97_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07623.1"
                     /translation="MVRELEAAGRLDTSAGLAAVVLAQRIAAGEDTGAGIAAMVKQLG
                     ATMADALRGVAVEGDPLDELRARRAAKETATAADA"
BASE COUNT          296 a          810 c          691 g          258 t
ORIGIN      
        1 agcgcggcgg ccagcaggcc cagcccggca cccaccggtc cgccgacaag cccggcgacg
       61 atgaggcacg cgcccgcggc cgcgcacagc gccgccaggc cctcaaggag ggtcgtcacc
      121 cagtcgagca tccagcctcc tgtctcacca cgccgaatcc aagatgtcgt aggccacggc
      181 gcggcccagc cacatccagt gcgcatacgt cgccgcgacc agcggcgaca cgtcgacggt
      241 cgagtccttc cgcgaccact tccagccgtc gcccacggtg cgccgcgacg cgccgcccac
      301 cgccgccatc agcgccggct ccccccggtg gcgcacagcc gcatcggcga cggctgtcac
      361 catcgccccg cacgcgcgga ccgtgtcccg cccgtcgagg aggcgaaccg gcaccccggc
      421 gcgctccaga tccggcagca gcgacccgac aggccccgca gggtcgatgc cgatctccgc
      481 tatccggtgc cgatccctga gctctgcgag ccgttcgggc agccatgacg tgccgcggcg
      541 gtgctcgacc agctccagga tcccggcgcc gcacaccacc accgacgacc acaccccgtt
      601 gggtgccacg tcggcggcgg cgaccagatc gcccgtcggg ccgtcctccg tctcggccgc
      661 ggcccactcc gccccggtca ggtcgtccgt ggagtcggcg gggtcctccc accagccgag
      721 gaactcgcgc gagaactcat ccggcggcat cccggactgg cggaacgcca gcatgatgtc
      781 ctcggtgatg cgccgcccca gcgcgggcat cgcccggcgc caccggcccc ggtcgtccag
      841 ggagcatccg gcggtgccgt acccgtgctc gcagccgtcc accgcacacg cgggcgggtc
      901 cgggtcgcac cactccaggt acgccagccg cggcttgttc ccggcgcggc ccgagtcgcg
      961 gatgccgcgg agcaccgccg catccagccc gccggcagac gacgcgtaca cgacctgcgc
     1021 catcggccgg gtcgtcaacg tcggcagaag cgcacccatc tgagcggggc gcagcgcgaa
     1081 cgcctcatcg aggatgacca cgtcgccggt gaggccgcgg ccgccggcct tcgtgcgggc
     1141 cttgaacttc aggcggcagc cgttgagcag ctcgatcgcc tcgtcgccgt tggcccggtg
     1201 cactgccttg accttgcggt ccagctccgg cgtgccctcc accagctcca gcatgtcgcg
     1261 gaacgcctcc tgcgccgtgg agaactcgtg cgcggaccac acgatgaggc gccggtccag
     1321 caggaacatc cagcccagcg caccctgctt cagggttccg gtcttcaagt tctgccgcgg
     1381 tgcgacgatc gcggtctcga acgctgctgg tgtccccgaa ggggtcacgg cgaacatctg
     1441 gtcaaggcac agccgctgtt ccgggtccgg cggcagcccc gccatcgccg cgagctcggc
     1501 gacctccggg ccgtacgtcc cggccgcatc caccagatgc gagtagaccg gcggcttaag
     1561 catcggcggc cgtggccgtc tccttcgccg cacggcgggc acgcagctcg tcgagcgggt
     1621 cgccctccac cgctacaccg cgcagcgcgt cggccatcgt cgcgccgagc tgcttcacca
     1681 tcgcggcgat accggcgccg gtgtcctcgc cggcggcgat gcgctgcgcc aggacgacgg
     1741 cggccagccc ggcggacgtg tccagcctcc cggcggcctc cagctcgcgc accacagcgc
     1801 cggccagcgt cccagccttc ggggcgggcc tcgcagcgat gtcgcgggcg gcctgcgaag
     1861 caccgcgccg gggcgcgcag tccaggcact tcgtgcgcgg ccggccacgg ccgggaggcg
     1921 gcagcggccc gccgcagatg gcgcacgtgc gaggcattgg gcggctccgt aggcgaaatt
     1981 gaattcgtgg ggagagaaac taggaagcgg gcggtttgcc atcacaatgg cgttgcggtg
     2041 gaagaacaag ttctt
//
LOCUS       DAOQNN010000528         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_46039_length_2053_cov_8.864364, whole genome shotgun sequence.
ACCESSION   DAOQNN010000528 DAOQNN010000000
VERSION     DAOQNN010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46039_length_2053_cov_8.864364"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKV97_16945"
     CDS             complement(<1..680)
                     /locus_tag="PKV97_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNB07624.1"
                     /translation="MKEKHYLTPLLEPRSVGIIGASEREASLGSVLMRNMLEAGYKGK
                     LFAINPKHEKVHGVACYKSVEDVPQRLDLVVMAIRAEKTPALMEACGRAGVKAVILLS
                     GGFSESGARGALLERQVVEAAHRHRIRLLGPNCLGVMRPQLGVNATFAHASALKGSIG
                     LISQSGALCAAILDWAKPNNVGFSTVVSLGSSSDIDFGEVLEYMISDPRTESIFLYVE
                     GIRDARRF"
     gene            complement(879..>2053)
                     /locus_tag="PKV97_16950"
     CDS             complement(879..>2053)
                     /locus_tag="PKV97_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB07625.1"
                     /translation="LLPAAHAAPSAPAGAGAAIGVIATESYREVVDSLSEVLFRTDEL
                     GRLVFLNDAWKDLSGFDLDATRQHALTDFLHPDDRLRARDAIRALLCGDGQEWTDELR
                     LRTLSGEIRWVAIDCRALRDGSGQESGIAGTIDDISARKIAEFSLRNLNQELESRVRS
                     RTAELETAVRELEAFSYSVSHDLRAPLRAIDGFARILVEEAGPRLDERQREQLVRIRA
                     GAERMAILIDALIDLASVSRQPLRRRPVDLSRIAEAVIRDLQAESPGRVVAVEITSDM
                     TVVADPVLMHVLLDNLLRNAWKFTSQCEHPRIVFGAERDGERTVFHVEDNGAGFEMNY
                     AGKLFQPFQRLHAQHEFPGTGIGLATVQRIVARHEGRVWASAEPGKGARFCFMLGH"
BASE COUNT          313 a          675 c          727 g          338 t
ORIGIN      
        1 atgaagcggc gggcgtcgcg gatgccttcg acgtagagga agatgctctc ggtgcgcggg
       61 tccgagatca tgtattcaag cacctcgccg aagtcgatgt cggacgagga gcccagggac
      121 accacggtcg agaagccgac gttgttcggt ttggcccagt cgaggatggc cgcgcacagc
      181 gcgcccgact gcgagatcag gccgatgctg cccttgagtg cgctggcgtg cgcgaaggtg
      241 gcgttgacgc cgagctgcgg ccgcatgacg cccagacaat tggggccgag caggcggatg
      301 cggtggcggt gcgcggcttc gaccacctgg cgctcgagca gcgcgccgcg cgcgcccgat
      361 tcggagaagc cgcccgacag caggatcacc gccttcaccc cggcgcggcc gcaggcctcc
      421 atcagcgccg gggtcttctc ggcgcggatt gccatcacca ccaggtccag gcgctgcggc
      481 acgtcttcca ccgacttgta gcaggcgacc ccgtgcacct tctcgtgctt cgggttgatc
      541 gcgaacagct tgcccttgta gccggcctcg agcatgttgc gcatgagcac gctgccgagg
      601 gaggcctcgc gctcgctggc gccgatgatg ccgacggagc gcggctcgag gagcggggtc
      661 agatagtgct tttctttcat ggtggccacc tgaggtccga gtggtgtgct gcaatggttt
      721 ggcgggcgca gggcgcgcgc ggaatggaat tcattgtact gcagtgcaca acgggcagtt
      781 gagacctttg tctctgccgc ctgcgattcg tgccgcggcc gaccgaccgc cgctgtccgg
      841 agtgggcttt cccgtttccg agcgcggcgt cgtcaccctc aatgccccag catgaagcag
      901 aagcgcgcgc ccttgcccgg ttccgcgctg gcccacaccc ggccctcatg gcgggcgacg
      961 atgcgttgta cggtggcgag cccgatcccg gtgccgggaa actcgtgctg cgcgtgcagg
     1021 cgctggaagg gctggaagag cttgcccgcg tagttcatct cgaatccggc gccgttgtcc
     1081 tcgacgtgga acaccgtgcg ctcgccgtcg cgttccgcgc cgaagacgat acgcggatgt
     1141 tcgcactggc ttgtgaactt ccatgcattg cgcagcaggt tgtcgagcag cacgtgcatc
     1201 agcaccgggt cggcgaccac cgtcatgtcg ctggtgatct cgaccgcgac cacgcgcccg
     1261 ggcgactccg cctgcaggtc gcggatcacc gcttcggcaa tgcgcgacag gtcgaccggc
     1321 ctcctgcgca acggttgccg ggagacgctg gcgaggtcga tcagcgcatc gatcaggatc
     1381 gccatgcgct cggcgccggc gcggatgcgc acgagctgct cgcgttggcg ttcgtcgagc
     1441 cgcggcccgg cctcctcgac gaggatgcgc gcgaagccgt cgatggcgcg cagcggcgca
     1501 cgcaggtcgt gcgagacgga atacgagaag gcctcgagct cgcgcaccgc ggtctcgagt
     1561 tcggcggtgc gcgagcgcac ccgcgactcg agctcctggt tcaggttgcg caggctgaac
     1621 tcggcgatct tgcgcgccga gatgtcgtcg atggtgccgg cgatcccgct ttcctgcccg
     1681 ctgccgtcgc gcaaggcacg gcagtcgatc gccacccagc ggatctcgcc ggagagcgtg
     1741 cgcagccgca actcgtcggt ccattcctgg ccgtctccgc acagcagggc gcggattgcg
     1801 tcgcgtgcgc gcaagcggtc gtctggatgg aggaaatcgg tcagcgcatg ctggcgcgtg
     1861 gcgtccagat cgaaaccgga gagatccttc caggcgtcgt tgagaaacac caggcggcca
     1921 agctcgtcgg tgcggaagag cacctcgctg agcgaatcga ccacctcgcg ataggattcg
     1981 gtcgcgatca ccccgatcgc cgcgcctgcg cccgcgggcg cggacggagc ggcgtgcgcg
     2041 gcggggagga ggg
//
LOCUS       DAOQNN010000529         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_46079_length_2052_cov_8.478718, whole genome shotgun sequence.
ACCESSION   DAOQNN010000529 DAOQNN010000000
VERSION     DAOQNN010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46079_length_2052_cov_8.478718"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PKV97_16955"
     CDS             complement(<1..551)
                     /locus_tag="PKV97_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB07626.1"
                     /translation="MRAMPTDFPRLPGEPRTPPPTAFDQRDVPLGDAAHRVLEHVFGY
                     PAFRGEQGEIVEHVAGGGDALVLMPTGGGKSLCYQIPALLRHGTAIVVSPLIALMQDQ
                     VSALVEAGVRAAFLNSSLDMERARAVERALWDGELELLYVAPERLMTPRFLDQLDHLR
                     DTGRLSLFAIDEAHCVSQWGHDFR"
     gene            complement(626..1099)
                     /locus_tag="PKV97_16960"
     CDS             complement(626..1099)
                     /locus_tag="PKV97_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNB07627.1"
                     /translation="MKGLSKGSLFFCALLSAAAGPAFADALIDATKPERLYEIARGFG
                     SAELDKDSQGDPRITGRIEGTKYGIYFYGCVKGADCDDIQFSASWSGPKVSLEKINEW
                     NRTKRFGKAYLDKDGDPNLEMEVNLDYGVSAKNIEDSFNWWTKALKEYKKVVLEE"
     gene            complement(1303..>2052)
                     /locus_tag="PKV97_16965"
     CDS             complement(1303..>2052)
                     /locus_tag="PKV97_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HNB07628.1"
                     /translation="KLRMIRRLLDHGRRPARIVGASVEELSSMLDSCLCPEGGTPAGR
                     DDGLLQLVRLHRSVELAAALRQALMKQGLQRFVAETVAPLAAAVGEAWMRGEVDVPEE
                     HLFTEQVQNVLRSAIGAHAATEGRPRVLLTTFPEEEHVLGLLMAEAMLVPEGASCLSL
                     GTRTPLADIRSAAVGGGFDIVGLSFSIAYPARQAIDGLLELRALLPEEIGIWAGGGAV
                     RDKQKRLPGIRVVGDLADTLAALQEWRKASA"
BASE COUNT          319 a          709 c          696 g          328 t
ORIGIN      
        1 cggaagtcgt ggccccactg cgacacgcag tgcgcctcgt cgatcgcgaa cagcgagagc
       61 cggccggtgt cgcgcaggtg gtcgagctgg tcgaggaagc gcggtgtcat caggcgctcg
      121 ggggcgacgt agagcagctc gagctcgccg tcccagagcg cgcgctccac cgcgcgtgcg
      181 cgctccatgt ccaggctgga gttgaggaag gcggcgcgca cgccggcctc gaccagcgcg
      241 ctcacctggt cctgcatcag cgcgatcaac ggcgacacca cgatcgcggt gccgtggcgc
      301 agcagcgccg ggatctggta gcacagcgac ttgccgccac cggtcggcat cagcaccagc
      361 gcatcgccgc cgccagccac gtgctcgacg atctccccct gctcgccgcg gaaggcgggg
      421 tagccgaaga cgtgctcgag cacgcgatgc gcggcgtcgc cgaggggaac gtcgcgctgg
      481 tcgaacgcgg tcgggggggg cgtgcgtggt tcgccgggca ggcgggggaa atcggtgggc
      541 atggcgcgca ttttaccgcg ccgggtctcg tccgatgaca cggaccgggg cagccgggag
      601 tctgcgacgc ccgggcccgc atccgtcatt cctcgaggac gaccttcttg tattccttga
      661 gggccttggt ccaccaattg aaggagtctt cgatgttctt ggcgctgacg ccgtagtcga
      721 ggttcacctc catctccagg ttcggatcac catccttgtc gaggtacgcc ttgccgaagc
      781 gcttggtccg gttccattcg ttgatctttt ccagcgagac cttggggccg ctccagctgg
      841 cggagaactg gatgtcgtcg cagtcggcgc ccttcacgca gccgtagaaa tagatgccgt
      901 acttcgtgcc ctcgatcctt ccggtgatgc gcggatcccc ctggctgtcc ttgtcgagct
      961 cggcggagcc gaatcctcgg gcgatctcgt agaggcgttc gggcttggtc gcgtcgatca
     1021 gcgcgtcggc gaacgcggga ccggctgctg cgctcagcag ggcgcaaaaa aacagacttc
     1081 ccttcgaaag tcccttcatg gaatcactcc tattgtggaa acacgggcgg tggcgcatct
     1141 ggcagaagcg tgcgccctga cggacgcgcg cgagcgcaag aatatgcttg cgagattcta
     1201 cggggcggct tggcgcggat gccgtgaagt tttaccgcac atgaacccgg aataggcccg
     1261 tcgtcagcgg gcgcgcgcac ggtcgcaggg cgcgtgcggc gctcaggcgc tcgccttgcg
     1321 ccattcctga agtgccgcca gcgtgtccgc caggtcgcct accacacgga tgcccggcag
     1381 gcgcttctgc ttgtcgcgga ccgcgccgcc gccggcccag atcccgattt cctccggcag
     1441 cagcgcgcgc agctcgagca agccgtcgat cgcctggcgt gccggatagg cgatgctgaa
     1501 ggacagcccg acgatgtcga aacctccacc caccgccgcg ctgcggatgt ccgcgagcgg
     1561 cgtgcgcgtg ccgagcgaca ggcagctcgc accctccggc accagcatcg cctccgccat
     1621 cagcaggccg agcacgtgtt cctcctcggg aaaggtcgtc agcagcacgc ggggccggcc
     1681 ttcggtcgcg gcatgcgcac cgatcgcgct gcgcagcacg ttctgcacct gctcggtgaa
     1741 cagatgctct tcgggaacgt ccacctcgcc gcgcatccac gcctcgccca ccgccgccgc
     1801 cagcggcgcc accgtctcgg cgacgaagcg ctgcaggccc tgcttcatca gcgcctggcg
     1861 cagtgcagcg gcgagctcca cgctgcgatg caggcgcacg agctgcagca gaccgtcgtc
     1921 gcgaccggca ggcgtgccgc cctccgggca caggcaggaa tcgagcatcg aagacagctc
     1981 ctcgacgctc gcgcccacga tccgggccgg tcgacggccg tggtcgagca ggcggcggat
     2041 catgcgcagc tt
//
LOCUS       DAOQNN010000530         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_46158_length_2050_cov_10.075689, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000530 DAOQNN010000000
VERSION     DAOQNN010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46158_length_2050_cov_10.075689"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKV97_16970"
     CDS             complement(<1..276)
                     /locus_tag="PKV97_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07629.1"
                     /translation="MAEIESPLAFLLTTPYASRYPRKLVEGFPHVARRIAELWDDKDK
                     LGEYFTELMVSKRPSRRGFPPEVGAEIVYLSMAYDLHGPVRPTPQAAP"
     gene            complement(434..1267)
                     /locus_tag="PKV97_16975"
     CDS             complement(434..1267)
                     /locus_tag="PKV97_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB07630.1"
                     /translation="MTTPHPEIRPGQSVELLKQLHILTRDGKLNQDSRRKLKQVYHLY
                     QFIEPLLAEALAERPDIELVDHGAGKSYLGFILYDLFFKAHAPGGRIHGIETRDELVT
                     SSRRLADKLGFSSGMRFQNLSVADSITSDRLPGRIDLVTALHACNTATDDAIRFALKK
                     DARAIVLVPCCQAEVAAALRRNKQQALRGPIAELWRHPIHTREFGSHVTNVLRCLQLE
                     AHGYEVTVTELVGWEHSMKNELIIARRQPERPRRRAAERLAALLGMLGLEELSERFFT
                     S"
     gene            1424..>2050
                     /locus_tag="PKV97_16980"
     CDS             1424..>2050
                     /locus_tag="PKV97_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB07631.1"
                     /translation="MSRTEASRNMGGHAPSAAAGVPPAFVVIIAGMVAALHIGKIPPA
                     IPVLRDALGVSLVEAGFLLSMVQMAGMLGGVFIGLAADGFGLRRSVLLGQGVLASASL
                     AGAWATQPGELLVLRAIEGLGFLLVVLPVPSLIRQLVPPSRLALHLGLWGTYMATGTS
                     LVLAGGPALMGVMGWQGLWGLLAMASAAVAAWLAVRVPSDHARRAAAGT"
BASE COUNT          256 a          661 c          793 g          340 t
ORIGIN      
        1 gggggccgct tgcggggtgg gacgcaccgg gccgtggagg tcgtaggcca tgctcagata
       61 aacgatctct gcgcccacct cgggtgggaa accgcgccgg ctcggccgtt tcgagaccat
      121 gagctcggtg aagtattcgc ccagcttgtc cttgtcgtcc cagagctccg cgatgcggcg
      181 ggcgacatgg ggaaagccct cgacgagctt gcgcggatag cggctcgcgt acggcgtggt
      241 cagcaggaat gccagcggtg attcgatttc ggccatgtcc gttccggatg cgcccgcatc
      301 cgtcctcctc gactttccgg ttccgtccgg gcgcggcgcc ctgtctcctg tccaccttgc
      361 gtggcggact ccccgattct agcagttcga tcgcagcaca aaggcatgga tcggcagtgt
      421 cggcgcggac gggtcagctc gtgaagaaac gctcggaaag ttcttccaga ccgagcatcc
      481 cgagcagcgc ggcgaggcgt tcggccgcgc gccggcgcgg gcgctcgggc tggcggcggg
      541 cgatgatcag ctcgttcttc atcgagtgct cccagcccac gagctcggtg accgtgacct
      601 cgtagccgtg ggcctcgagc tgcaggcagc gcagcacgtt ggtgacgtgg ctgccgaact
      661 cgcgggtgtg gatggggtgg cgccacagct ccgcgatcgg gccgcgcagc gcctgctgct
      721 tgttccggcg cagcgccgcg gcgacctcgg cctggcagca cggcaccagc acgatcgcgc
      781 gggcatcctt cttcagcgcg aagcggatgg cgtcgtcggt ggcggtgttg caggcgtgca
      841 gcgcggtgac gaggtcgatg cgtccgggca ggcggtccga ggtgatcgag tccgccacgc
      901 tgaggttctg gaagcgcatc ccggacgaga agccgagctt gtcggcgagc cggcgcgagc
      961 tcgtcaccag ctcgtcgcgc gtctcgatgc cgtggatgcg gccgccgggg gcgtgcgcct
     1021 tgaagaacag gtcgtagagg atgaagccca ggtaggactt gccggcgccg tggtcgacca
     1081 gctcgatgtc ggggcgctcg gccagggcct cggcgagcag cggttcgatg aactggtaga
     1141 ggtggtagac ctgcttgagc ttgcgccggc tgtcctggtt gagcttgccg tcgcgggtca
     1201 ggatgtggag ctgcttgagc agctcgaccg actggccggg gcggatttcg gggtgcgggg
     1261 tggtcatggc gggcggggcg cgcgggcgcg gaagcggggt gggcgcaaat gtacacggcg
     1321 gggggcggac caccaagccg cgaacgcggc gggctcgccc gcgcgcacgc cggcgcgatc
     1381 gcggctatgc tctcgtcgcc catcgagagg ctgagaggag accatgagcc gcacagaagc
     1441 ttcccggaac atgggcgggc acgcgccgtc cgcggctgcg ggcgtcccgc ccgccttcgt
     1501 cgtcatcatc gccggcatgg tcgccgcgct gcacatcggc aagatcccgc cggcgatccc
     1561 ggtgctgcgc gatgcgctcg gcgtgagcct ggtcgaggcc ggcttcctgc tctccatggt
     1621 gcagatggcg ggcatgctcg gcggcgtctt catcggcctg gccgcagacg gcttcggcct
     1681 gcgccgcagc gtgctgctcg gccagggcgt gctcgcctcc gccagcctcg ccggcgcgtg
     1741 ggcgacccag cccggcgagc tgctggtctt gcgcgcgatc gaggggctgg gcttcctgct
     1801 cgtggtgctg ccggtgccca gtctgatccg ccagctcgtg ccccccagcc ggctggcgct
     1861 gcacctgggc ctgtggggca cctacatggc cacgggaacc tcgctggtgc tggccggtgg
     1921 gccggcgctg atgggggtga tgggctggca ggggctgtgg gggctgctgg cgatggcctc
     1981 ggctgcggtg gcggcgtggc tggcggtgcg ggtgccatcc gaccacgcgc gccgtgctgc
     2041 ggcgggcacc
//
LOCUS       DAOQNN010000531         2045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_46362_length_2045_cov_9.318593, whole genome shotgun sequence.
ACCESSION   DAOQNN010000531 DAOQNN010000000
VERSION     DAOQNN010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46362_length_2045_cov_9.318593"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKV97_16985"
     CDS             complement(<1..323)
                     /locus_tag="PKV97_16985"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNB07632.1"
                     /translation="MLETFQTAGAATLGGAWPVVWALIKIVVVLLPLMGCVAYLTLWE
                     RKAISWTQVRPGPNRVGPYGLLTPIADAVKLIFKEVITPTVADKGLFYLGPIMTIMPA
                     LAAWAV"
     gene            complement(339..>2045)
                     /locus_tag="PKV97_16990"
     CDS             complement(339..>2045)
                     /locus_tag="PKV97_16990"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNB07633.1"
                     /translation="TSKPFRYSARTWELSRRKSVSPHDSTGANLIVQVKNHRVMRVVP
                     LENDEVNECWIADRDRFSYEALNSDARLTTPMLKQGGQWKAVDWNSALDYVAKALKGI
                     VAQQGPAAVGALVSATSTNEELFLLGELVRGLGSDNIDHRLRHADFRQEAGAVRWLGT
                     PIANLSKVDRALVVGSFLRKDHPLFAQRLRQAARHGAQILRIGSAGDDWLMPVAAQIA
                     TAPSLWATALIEVAAAVATAKGVTAPLAVTPGAQAQAIADALLSGQNTAVLLGNAAAA
                     HPQAAELLAVANWIAAQCGASVGYLGEAGNSVGAQLVKAQPQQGGLDAGAMLAQPRKA
                     YLLFNTEPLLDSADAAQAAKALAAADLVVSFSPFSANVEQANVLLPIAPFTETGGSFV
                     NAEGRLQSFHGVVRPLGETRPGWKVLRVLGNLLGLPGFAQESVEEVRAQAIGSADGIA
                     ARLSNATRVSPQGGVAQVELQRVADVAIYGSDSIVRRAPSLQQTADGRAIQVSLPASL
                     WGRLGLAEGDSVNVTQGGETVKLPAVLDAGLAEGTVRIPTGVAATAKLGALFGAVNVV
                     RA"
BASE COUNT          295 a          726 c          725 g          299 t
ORIGIN      
        1 accgcccagg ccgccagcgc cggcatgatc gtcatgatcg ggccgaggta gaacaggccc
       61 ttgtcggcca ccgtcggcgt gatgacttcc ttgaagatca gcttgacggc gtcggcgatc
      121 ggggtcagca ggccgtaggg gcccacgcgg ttcgggccgg ggcgcacctg cgtccaggag
      181 atcgccttgc gctcccacag cgtcaggtag gccacgcagc ccatcagcgg cagcagcacc
      241 acgacgatct tgatcagcgc ccagacgacg ggccacgcgc cacccagggt cgcggcgccg
      301 gcggtttgga atgtttccag catcttcagc tcccctcgtc aggcacgcac gacgttcacc
      361 gcgccgaaca gcgcgccgag cttggcggtg gcggccacgc ccgtcgggat gcgcacggtg
      421 ccctcggcca ggccggcatc cagcacggcc ggcagcttca ccgtttcgcc accctgggtg
      481 acgttgaccg aatcgccctc ggccagcccg agccgccccc acagcgaggc cggcagcgac
      541 acctgaatgg cgcggccgtc ggcggtctgc tgcagcgacg gtgcgcggcg cacgatgctg
      601 tcgctgccgt agatcgccac gtcagcaacg cgctgcaact cgacctgcgc gaccccgccc
      661 tgcggcgaca cgcgggtggc gttggacagg cgcgcggcga tgccgtcggc gctgccgatg
      721 gcctgggcgc gaacttcctc gacgctctcc tgcgcgaagc ccggcaggcc cagcaggttg
      781 cccagcacgc gcagcacctt ccagcccgga cgggtctcgc ccagcggacg caccacgccg
      841 tggaaggact gcaaacggcc ttctgcgttg acgaagctgc caccggtctc ggtgaacggt
      901 gcgatcggca gcagcacgtt ggcctgctcg acgttcgcgg agaaggggct gaacgacacg
      961 accagatcgg cagcggccag ggccttggcg gcctgggcgg cgtcggccga atccagcagc
     1021 ggctcggtgt tgaacagcag gtaggccttg cgcggctggg ccagcatcgc gccggcgtcc
     1081 agaccgcctt gctgcggttg ggccttgacc agctgcgcgc cgacgctgtt gccggcctcg
     1141 ccgaggtagc cgacgctggc accgcactgc gccgcgatcc agttggccac cgccagcagc
     1201 tcggccgcct gcgggtgcgc cgccgcagcg ttgcccagca gcaccgcggt gttctgaccg
     1261 gacagcaggg cgtcggcgat cgcctgggcc tgcgcacccg gcgtcacggc cagcggcgcg
     1321 gtcacgccct tggcggtggc gaccgcggcg gccacctcga tcagcgccgt cgcccacagg
     1381 ctcggcgccg tggcgatctg ggcggcgacc ggcatcagcc agtcgtcgcc ggccgagccg
     1441 atgcgcagga tctgcgcgcc gtggcgggcg gcctgacgca ggcgctgagc gaacagcggg
     1501 tggtccttgc gcaggaagga gccgacgacc agcgcgcgat cgaccttcga caggttggcg
     1561 atcggcgttc ccagccaacg cacggcaccg gcttcctggc ggaaatcggc gtggcgcaga
     1621 cggtggtcga tgttgtcgct gcccaggcca cggactagct cacccagcag gaagagctct
     1681 tcgttggtgc tcgtggccga aaccagggcg ccgacggccg ccggaccctg ctgggcgacg
     1741 atgcccttga gcgccttggc gacgtagtcc aacgcgctgt tccagtcgac ggccttccac
     1801 tgaccgccct gcttgagcat cggcgtggtc aggcgggcgt cgctgttgag cgcctcgtag
     1861 ctgaagcggt cgcggtcggc gatccagcac tcgttgacct cgtcgttctc cagcggaacg
     1921 acgcgcatca cccggtggtt cttgacctgg acgatcaggt tggcaccggt gctgtcgtgc
     1981 gggctgacgc tcttgcggcg cgacagctcc caggtgcggg cgctgtagcg gaagggcttg
     2041 ctggt
//
LOCUS       DAOQNN010000532         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_46804_length_2034_cov_10.222335, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000532 DAOQNN010000000
VERSION     DAOQNN010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46804_length_2034_cov_10.222335"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKV97_16995"
     CDS             complement(<1..520)
                     /locus_tag="PKV97_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB07634.1"
                     /translation="MNPQVTFASLGLAEPILRAVSEAGYSTPTPIQAKAIPQVLAGGD
                     LLAAAQTGTGKTAGFTLPLLHILSQTHTHPHPPGRPRCLILTPTRELAAQVQESVDTY
                     GKHLPLSSLVMFGGVNINPQISALKKRVDILVATPGRLLDHVGQKTLDLSGVEILVLD
                     EADRMLDMGFIRD"
     gene            825..1979
                     /locus_tag="PKV97_17000"
     CDS             825..1979
                     /locus_tag="PKV97_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB07635.1"
                     /translation="MREIQLSEDLDGRYDRLSRDLGARALAGLLAVSGSILVLLGGAV
                     VGAVATWEAIGRGTPVETLFWVLFMLGSIAAIWATLASLASLAPRPCGLAVARERVGE
                     LYALVDALAHVAGVSPIRRIYVTGEINASIVQRPRHLGVGEMATDLLIGLPLAHALGP
                     RQLAAVIAHEIGHMVARQRGMHGRSGWLQAWWMRTLDELAAALPSGFPWFDPQGDALC
                     MRMLLLAQMEEYAADRTAVRLVGAELLAGTLVELCCKADFLANDYLPRVHALAECDEA
                     SCVRPYREMGHGFAAGFAQSSAAIDPQRVLRADAGDPFHPALQDRLVAIGVAMPERLQ
                     VSGPSAAQHFFGDSLPFLAWHFDRIWLESLRDACPPALSPRAPERVLQGA"
BASE COUNT          262 a          675 c          764 g          333 t
ORIGIN      
        1 tgtcgcggat gaagcccatg tcgagcatgc ggtcggcttc gtccagcacc aggatctcga
       61 cgccggagag atcgagcgtc ttctggccga cgtggtcgag caggcggccg ggggtggcga
      121 cgaggatgtc cacccgcttc ttcagtgcgc tgatctgcgg gttgatgttc acgccgccga
      181 acatgaccag cgaggacagc ggcaggtgct tgccgtaggt gtcgaccgac tcctgcacct
      241 gggcggcgag ctcgcgcgtg ggggtcagga tcaggcagcg cgggcgcccg ggcgggtgcg
      301 gatgggtgtg ggtctgcgac aggatgtgca gcagcggcag cgtgaagccg gcggtcttgc
      361 cggtgccggt ctgcgccgcc gcgagcaggt cgccgccggc gagcacctgc gggatggcct
      421 tggcctggat gggcgtgggc gtggagtaac ccgcctcgga gaccgcgcgc aggatgggtt
      481 cggccagccc gaggctggcg aaggtgactt ggggattcat tcgatggtcc ggcgcgagcc
      541 cgtcgcatgc atcgatgcga caccaatccg ggctgcggga gggatccgcg ccgcatgggg
      601 gcgaacggat cgttgcagga aacgcggcgg gtgactggtg caccgcagcg aagccggcac
      661 tataagcgct ccacaccgga atggcgagcc atttcggccc gctggcgacc gcgaatccgc
      721 ccccgcggcc tcgggggcgc tgacgaatct tgcgctggat cgtgtcatgg catgaatgtt
      781 tggttacaat ccgacacaac ccggttcccg gcaggggtat tcccgtgcgc gaaatccagc
      841 tctccgaaga cctcgacggc cgctacgatc ggctctcccg cgatctgggc gcgcgcgcgc
      901 tagccggcct gctcgctgtg tccggttcga tcctcgtcct gctcggcggg gcggtcgtgg
      961 gtgccgtggc gacctgggag gcgatcggcc ggggcacacc ggtggaaacg ctgttctggg
     1021 tgctcttcat gctcggcagc atcgccgcga tctgggccac gctggcctcc ctggcgagcc
     1081 tcgcgccgcg gccctgcggg ctggcggtcg cgcgcgaacg ggtgggcgag ctctacgcgc
     1141 tggtcgatgc gctcgcgcac gtggcggggg tgagcccgat ccggcgcatc tacgtgacgg
     1201 gcgagatcaa cgcctcgatc gtgcagcgcc cgcgccacct gggtgtcggc gagatggcga
     1261 ccgatctgct gatcggcctg ccgctggccc atgcgctcgg cccccgccag ctcgcggcgg
     1321 tgatcgccca cgagatcggc cacatggtcg cacgccagcg cgggatgcac ggccggagcg
     1381 gttggctgca ggcgtggtgg atgcgcacgc tggacgagct tgcggccgcg ttgccgtccg
     1441 gctttccctg gttcgacccc cagggcgacg ccctgtgcat gaggatgctg ctgctggcgc
     1501 aaatggagga gtacgccgcc gaccgcaccg cggtgcgcct ggtaggggcc gagctgcttg
     1561 ccgggaccct ggtcgagctc tgctgcaagg cggacttcct cgccaacgac tacctgccgc
     1621 gcgtgcatgc cctggccgag tgcgacgagg cgtcttgcgt gcggccctac cgcgagatgg
     1681 ggcacggctt tgccgccggc ttcgcccaat cgagcgctgc catcgatccg cagcgcgtgc
     1741 tgcgcgcgga cgccggcgac cccttccacc ccgcgctgca ggatcgcctc gtggcgatcg
     1801 gcgtcgccat gcccgagcgg ctgcaggtgt cgggtccttc tgcggcgcag catttcttcg
     1861 gcgacagcct gcctttcctg gcctggcatt tcgaccggat ctggttggag agcctgcgcg
     1921 acgcttgccc gccggcgctc agtccgcgcg cgccggagcg ggtgctgcag ggggcgtgag
     1981 caccgcggag gcggcgccgc ggcgcttgcg tgcggtctcg acctggccgc acag
//
LOCUS       DAOQNN010000533         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_47137_length_2026_cov_14.907154, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000533 DAOQNN010000000
VERSION     DAOQNN010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47137_length_2026_cov_14.907154"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1226
                     /locus_tag="PKV97_17005"
     CDS             126..1226
                     /locus_tag="PKV97_17005"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HNB07636.1"
                     /translation="MALELSKSTWKVGFEYQGKRRIKNVTGGEVGELRQALDEAMQKL
                     GVPAEALVRCCYEAGRDGFWLHRLLEDWGVENVVVDPASIEVSRQARRTKTDRVDLLS
                     LLSKLRQYHAGEFGVWKVVKVPSEQDEEGRLLHRELERLKKERTGHVLRIKALLFAQG
                     VRVEQVGGKKWAGYLMGLTRYDGRALPADLMAQVRRESERLAMVQEQIQAIEKEQERR
                     VREAAEVGGVMAMVQALRQLKGIGSVGAWVFVMEFFGWRQFKNGKEVGALAGLTGTPY
                     ASGQMQREQGISKAGNRRVRALAVEIAWLWLRYQPDSALSRWYAERFAGTSKRHKRVG
                     IVAVARKLLVALWRYLQTGELPTGARLKPAKA"
BASE COUNT          369 a          541 c          809 g          307 t
ORIGIN      
        1 tgtagaaact atgaagggcc atccggtctc tctccatctg aacccgttgc gcgggagaac
       61 aggaggaaaa gatggcacag atggcccttc aagcgaacga tagtggattg gttggggtgt
      121 tgtacatggc gctggagctg agcaagtcga cgtggaaggt cggcttcgag taccagggca
      181 agcggcggat caagaacgtg accgggggcg aggtgggcga gctgcgccag gcgctcgacg
      241 aggcgatgca gaagctgggc gtgcccgcgg aggcgttggt gcgctgctgc tacgaagcgg
      301 gccgggacgg gttctggctg caccggctgc tcgaggactg gggcgtggag aacgtggtgg
      361 tcgatccggc gagcatcgag gtgagtcggc aggcgcgccg gacgaagacg gatcgggtgg
      421 atctgctgtc gctgctgtcg aagctgcgcc agtaccacgc gggcgaattc ggggtttgga
      481 aggtggtgaa ggtgccgtcg gagcaggacg aggaaggacg gttactgcac cgcgaactcg
      541 agcggctcaa gaaggagcgc acgggccatg tgttgcggat caaggctttg ctgttcgccc
      601 agggggtgcg ggtcgagcag gtgggcggca agaagtgggc cggttatctg atggggctga
      661 cgcgctatga cggtcgtgcg ctgccggccg acctgatggc gcaggtgcgt cgcgagagcg
      721 agcgcttggc gatggtgcag gagcagatcc aggcgatcga gaaggagcag gagcggcgcg
      781 tgcgcgaggc ggccgaggtg ggcggggtga tggcgatggt gcaggcgctg cgccagctca
      841 aggggatcgg ctcggtgggg gcgtgggtgt tcgtgatgga gttcttcggc tggaggcagt
      901 tcaagaacgg caaggaggtg ggggcgctgg ccgggctgac gggcacgccg tacgcgagcg
      961 gtcagatgca gcgcgagcag ggcatcagca aggcgggcaa ccggcgggtg cgggcgctgg
     1021 cggtcgagat tgcgtggttg tggctgcgct accagcccga cagcgcgctc agccgctggt
     1081 acgccgagcg ctttgccggc acgagcaagc gccacaagcg ggtgggcatc gtggcggtgg
     1141 cacgcaagct gctggtggcg ctgtggcgct acctgcaaac aggcgagctc cccacgggcg
     1201 cccggctcaa gccggccaag gcgtgaggcg gaatgattta tgcatgaagc acgattgagc
     1261 tagacggagg gaggcgccgc aacgcaggcc cggtggaacg gtcgatgcgt gcgagcacag
     1321 gttgaagaag gtttcgcggt cgcccgaggc gctcctgggt gcatgaatgc accgatcttg
     1381 tagaaggggc accgcacacg attggtcgga ctgccgcctg cgggcgtatg cagttttagg
     1441 tcaccgcagc ccgaaggggc gcggtacgga tagaaggtgg tgacaggctc agtcctctca
     1501 cgcgatggat cgaccaatga aaaccttgca tcgacgatgt gtgcagcacg atggaccgca
     1561 acacggcagg ccggcgcgag gaggaaacga tgtcgaacaa gacgggcaag tggttgccgc
     1621 agggtcgtcg gcccggtgtg gccggaccga cgaccctgcg cgagcctaag gctcccttca
     1681 cggcgtcaag ggtgcgctac gccggtccgc ttcgcggccc gcccttgacc ccgttccggg
     1741 ggtagaaacc gacaaccgga gagggttgac aacggaggcc tcatagaagg agcgccggca
     1801 ggcgcgaaag cggcggagaa gccctgcacg cgggtcgctt gccgggtcgc cgggttcgca
     1861 gctgccgcag cacctacaag cgggcgagaa gcgcggtggg ttctgtagga gccggaaggc
     1921 gcgaatcgtg cgctcgagcg gcgccggcgc gcattccatg cgccgcatcc acggcttcag
     1981 ccgatcaggc ggcggtgctc gatcttggtg cagcggtcgc tcacca
//
LOCUS       DAOQNN010000534         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_47140_length_2026_cov_6.768138, whole genome shotgun sequence.
ACCESSION   DAOQNN010000534 DAOQNN010000000
VERSION     DAOQNN010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47140_length_2026_cov_6.768138"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /gene="miaA"
                     /locus_tag="PKV97_17010"
     CDS             complement(<1..410)
                     /gene="miaA"
                     /locus_tag="PKV97_17010"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNB07637.1"
                     /translation="MPPPIPAPLPPALLLLGPTASGKTASALALAQALPVEVISVDSA
                     LVYRDMDIGTAKPNAEERAVCPHHLIDVVTPEESYSAARFRADAIRLMGEIVARGNIP
                     LLAGGTMLYFKALRDGLSDLPAADPELRRALDDEA"
     gene            complement(624..1187)
                     /locus_tag="PKV97_17015"
     CDS             complement(624..1187)
                     /locus_tag="PKV97_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNB07638.1"
                     /translation="MRKPAILAAALAAAVLVTAGCATEPVASRKDISFDAAAANPLIP
                     SNYAAADSLLAQLRGQLAPTQALIAATVVNIDALEQSSTLGRLISEQVSARFTLAGYR
                     MIEMKFRNNVYMARDQGELMLTREIRDLASTHDAQAVVVGTYAQSREMVFVNLKVIQP
                     ETNVVLAVHDYALPLDPMTRSMLRGHR"
     gene            complement(1187..1888)
                     /locus_tag="PKV97_17020"
     CDS             complement(1187..1888)
                     /locus_tag="PKV97_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07639.1"
                     /translation="MIRSLTAAATLALLATACATPYSPAPLATNFGTTKQQKLQAASH
                     WNTIARDVADKLSARIPTGSRLFVNQHSDASAFERAFASQLTTALVDAGHSVMRTPEG
                     AMRVGVETQAIAFTADRPQHRHAGLPTALGAGVWALYDIVEYASNGPAKAALAAVASA
                     DAYTWFQSEFASGDTPSMEIIVSASVTDASRYLARTSTAYYVSDSDQDLYLPHVEKQA
                     PRLPPMKSFQVVGGE"
BASE COUNT          314 a          615 c          754 g          343 t
ORIGIN      
        1 gcctcgtcgt cgagcgcgcg gcgcagctcg gggtcggccg cgggcaggtc ggagagcccg
       61 tcgcgcagcg ccttgaagta cagcatggtg ccgccggcca ggagcggaat attgccgcgt
      121 gcgacgatct cgcccatcag gcggatcgcg tcggcgcgga agcgtgcggc cgaatagctc
      181 tcctcgggcg tcaccacgtc gatcaggtgg tgcgggcaca ccgcgcgttc ctccgcgttc
      241 ggcttggcgg tgccgatgtc catgtcgcga tacaccagcg ccgaatccac gctgatcacc
      301 tccaccggca gggcttgcgc gagcgcaagc gcgctcgcgg tcttgccgct ggcggtgggg
      361 ccgagcagca gcagggcggg cggcaggggg gcggggatgg gcggaggcag tttgggcgag
      421 gccgaagtgt gcgctcgggc gcgggattcg cgacggcgca gggcgcgcgg attatctccc
      481 aggccgtcgt gatccgccag cgcgcgaccc gcaacccctc aggtttgccg agaacggcgg
      541 cgcatttcca ggctgatggg gccggtgccg aaaggcttgc ggtcggcggg ggcgcgcgtt
      601 cgcccgcccc cgggatgcgg cgatcagcgg tggccgcgca gcatcgagcg cgtcatcggg
      661 tcgagcggca gggcgtagtc gtgcaccgcc agcaccacgt tggtctcggg ctggatgacc
      721 ttgaggttca cgaacaccat ctcgcggctc tgcgcgtagg tgcccaccac caccgcctgc
      781 gcgtcgtgcg tgctggcgag gtcgcggatc tcgcgggtga gcatcagctc gccctggtcg
      841 cgcgccatat agacgttgtt gcggaacttc atctcgatca tgcggtagcc ggcgagcgtg
      901 aagcgcgccg agacctgttc ggagatgagg cggccgaggg tggaggattg ctcgagcgcg
      961 tcgatgttca ccacggtggc ggcgatcagt gcctgcgtcg gcgcgagctg gccgcgcagc
     1021 tgcgcgagca ggctgtcggc cgcggcatag ttggacggga tcagcgggtt ggcggcggcg
     1081 gcgtcgaagc tgatgtcctt gcgcgacgcg accggttcgg tggcgcagcc ggcggtgacg
     1141 agcacggcgg cggcgagggc tgcggcgagg attgcgggct tgcgcatcat tcgcctccga
     1201 cgacctggaa ggacttcatc ggtggcagtc tcggtgcttg cttctccaca tgcggcaggt
     1261 agagatcttg atcggagtcg ctcacatagt acgcggtgct cgtgcgagcg aggtagcgcg
     1321 atgcgtctgt aacactcgcg ctgacgatga tttccatcga aggcgtgtca cccgaggcga
     1381 actccgactg gaaccaggtg taggcatctg cagatgcgac ggcagcgagc gcagctttgg
     1441 ccggaccgtt cgaggcatat tccacaatgt cgtatagcgc ccacacgcca gccccgagcg
     1501 cggtggggag gccggcgtgt cgatgctggg ggcgatctgc cgtgaaggcg atcgcctgcg
     1561 tttccacgcc aacgcgcatc gcaccttcag gcgttcgcat gaccgaatgc ccggcatcga
     1621 ccagtgctgt cgtgagctgg cttgcaaacg cgcgctcgaa cgccgaggcg tcggagtgtt
     1681 ggttgacgaa cagcctagat ccagtaggta tgcgtgccga aagcttgtcg gccacgtccc
     1741 gggcaatggt gttccagtgc gaggccgctt gcaacttctg ttgcttcgtc gtcccgaagt
     1801 tggtggccag cggcgccggc gagtaagggg tggcgcaggc ggtggcgagc agcgcaagtg
     1861 tcgctgcggc ggtgaggctt ctgatcatga agcacattct ccgaaacggg cgttcagtcg
     1921 cccgctcggg cgacaacaac gctggggaag tgaaacgggg tattgctcgg ttatgccgaa
     1981 agctagcaga agccccgctc cgactcaatt gcgccgcatc ggcgga
//
LOCUS       DAOQNN010000535         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_47180_length_2025_cov_9.800000, whole genome shotgun sequence.
ACCESSION   DAOQNN010000535 DAOQNN010000000
VERSION     DAOQNN010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47180_length_2025_cov_9.800000"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PKV97_17025"
     CDS             complement(<1..424)
                     /locus_tag="PKV97_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB07640.1"
                     /translation="MDMTSQTPHFPIYMDYGATTPVDPRVVDVMVPWLREHFGNPASR
                     SHAWGWEAEEAVEKARTQVAELIGADPREIVWTSGATESNNLALKGAAHFYASRGKHL
                     ITVKTEHKAVLDTMRELERQGFEVTYLDVEADGLLDLEK"
     gene            complement(523..1056)
                     /locus_tag="PKV97_17030"
     CDS             complement(523..1056)
                     /locus_tag="PKV97_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNB07641.1"
                     /translation="MRLTTKGRFAVTAMIDLALREHSGPVALAAISARQQISLSYLEQ
                     LFGKLRRQELVESTRGPGGGYSLGRKAEDITVADIIVAVDEAIDATGCGGGENCMGDE
                     SGRCMTHDLWSSLNAKMLEYLNSISLKSLVDDQLSRGVSVEDAPIKRAISSQPVVKPI
                     KVTAPNSVFALAGTLTK"
     gene            complement(1197..1715)
                     /locus_tag="PKV97_17035"
     CDS             complement(1197..1715)
                     /locus_tag="PKV97_17035"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNB07642.1"
                     /translation="MTAPAPNRGPSRVLMVCMGNICRSPTAEAVLRHRVARHGLQGSV
                     EIDSAGTHAWHTRNPPDERSIAHAARRGYDLTPLRARRIEPEDFTRFDLLLAMDADNL
                     AHLREQAPATAQERLRLLMEFAPQLGSREVPDPYYGGPAGFERVLDLVEAACDGLIEH
                     LTRINRADRNFR"
     gene            complement(1712..>2025)
                     /locus_tag="PKV97_17040"
     CDS             complement(1712..>2025)
                     /locus_tag="PKV97_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNB07643.1"
                     /translation="PRGIRKQIKDLIDGVYSDKGGKDELKQLEAAAEVEALSEKDLGK
                     RIKQLEKQMLEHAKNLEFEKAARVRDQLALLREQAFGASGADNLSALVPTRPGAGRSA
                     A"
BASE COUNT          267 a          683 c          713 g          362 t
ORIGIN      
        1 acttctccag gtcgagcagg ccgtcggcct cgacgtcgag gtaggtcacc tcgaagccct
       61 ggcgctccag ctcgcgcatc gtgtccagca ccgccttgtg ctcggtcttc accgtgatca
      121 ggtgcttgcc gcgcgaggcg tagaagtgcg ccgcgccctt gagcgcgagg ttgttcgact
      181 cggtggcgcc gctggtccag acgatctcgc gcgggtcggc gccgatcagc tcggccacct
      241 gggtgcgggc cttctcgacg gcctcctcgg cctcccaacc ccaggcgtgg ctgcgcgacg
      301 ccgggttgcc gaagtgctca cgcagccagg gcaccatgac gtcgacgacg cgcggatcca
      361 ccggggtggt cgcgccgtag tccatgtaga tcggaaaatg cggggtctgc gaggtcatgt
      421 ccatggttgg cagtctcgaa ccgcagcctt cgcggccgca gcgacatccg caccgcgcca
      481 gccggcccgg tgctcgacgt cgatgaatgg aaacggtcgg gcttacttgg tcagcgtacc
      541 ggccagcgcg aacaccgagt tcggcgcggt gaccttgatc ggcttgacca ccggctgcga
      601 ggagatcgcg cgcttgatcg gcgcgtcctc gaccgacacg ccgcgcgaga gctggtcgtc
      661 gaccaggctc ttcagcgaga tcgagttcag gtactccagc attttggcgt tcaggctgga
      721 ccagaggtcg tgcgtcatgc agcgcccgct ctcgtcgccc atgcagttct cgccgccgcc
      781 gcagccggtg gcgtcgatgg cctcgtccac cgcgacgatg atgtccgcca cggtgatgtc
      841 ctcggccttg cggcccagcg aatagccgcc gccggggccg cgggtgctct cgaccagctc
      901 ctggcggcgc agcttgccga acagctgttc caggtaggac agcgaaatct gctggcgcgc
      961 gctgatcgcc gccagcgcca ccgggccgct gtgctcgcgc agcgccaggt cgatcatcgc
     1021 ggtgacggca aaacggccct tggtggtcag tctcatggca tcgaactcct gtgatcggga
     1081 ggggcgcgcg gtcagggtgg tcacgcgcca ggctccgtgt cgggtggacc ctcgcctctt
     1141 tcccgagtga tttactcgat tatagggcag ttcccgcgga cgtgctgggt ggcgcactat
     1201 cggaagtttc gatcggcgcg attgatccgg gtcaagtgtt cgatcaatcc gtcgcaggcc
     1261 gcctcgacca gatcgagcac ccgttcgaag ccggccggcc cgccgtagta cgggtccggc
     1321 acctcgcggc tgcccagctg cggggcaaac tccatcagca ggcgcaggcg ctcctgggcc
     1381 gtggccgggg cctgctcgcg caggtgggcg aggttgtcgg cgtccatcgc cagcagcagg
     1441 tcgaagcggg tgaagtcctc gggctcgatg cgccgcgcgc gcagcggcgt caggtcgtag
     1501 ccgcgccgcg ctgcgtgcgc gatgctgcgc tcgtcgggcg ggttgcgggt gtgccaggcg
     1561 tgcgtgcccg cggagtcgat ctcgaccgag ccctgcagcc catgccgggc cacgcggtgg
     1621 cgcagcaccg cctcggcggt gggcgagcgg cagatgttgc ccatgcagac catcagcacg
     1681 cggctggggc ctcggttcgg cgcgggcgcc gtcatgccgc actccgtccg gcgccaggcc
     1741 gggtaggcac cagcgccgag aggttgtccg cgccgctggc gccgaaggcc tgctcgcgca
     1801 gcagggcgag ctggtcgcgc acgcgcgcgg ctttttcgaa ttccaggttc ttggcgtgtt
     1861 cgagcatttg tttctcgagc tgcttgatgc gcttgcccag gtccttctcg gacagggctt
     1921 cgacctcggc ggcggcctcg agttgcttga gctcgtcctt gccgcccttg tcgctgtaga
     1981 cgccgtcgat caggtccttg atctgcttgc ggatgccgcg cgggg
//
LOCUS       DAOQNN010000536         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_47315_length_2022_cov_9.661922, whole genome shotgun sequence.
ACCESSION   DAOQNN010000536 DAOQNN010000000
VERSION     DAOQNN010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47315_length_2022_cov_9.661922"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PKV97_17045"
     CDS             complement(<1..793)
                     /locus_tag="PKV97_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNB07644.1"
                     /translation="MDCATSPSSAAAAALRTLALVGHPNVGKSVLFHRLTGTYVTVSN
                     YPGTTVEVSRATPRFDREASLLDTPGVLALPSRSDDERATMRALLHEDMRTLIQVGDA
                     KNLRRTLNLTALLAELGVPMVMALNMTDEAKARGVTVDAEALSEALGVPVVATVATGG
                     EGVSALTEALQRAAVPAPLLRYDPVLERDIARVKQLILAQCPHPRLAARGLAILAIGR
                     DPEVEAWLAAQANSETVRAALAELTAPIGRDSLPARLARERGGAAE"
     gene            1067..1678
                     /gene="rpsD"
                     /locus_tag="PKV97_17050"
     CDS             1067..1678
                     /gene="rpsD"
                     /locus_tag="PKV97_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNB07645.1"
                     /translation="MSRYTGPRLKVMRALGAELPGLSRKPLGERNYPPGQHGRRPRRK
                     SGFGVQLIEKQKLRFNYGLGEKQIQRLFREAKRDRGPTGDKLLELLERRLDNFVFRAG
                     FAPTAVAARQLVRHKHVLLNGRAVNIPSIRIRPGDRIQLTERARCIPVVVEALAEPAL
                     TRPEWLVFDKAAFVATMTRTPSPDEVPFPVDVQQVVEYYAVRL"
     gene            1675..1971
                     /locus_tag="PKV97_17055"
     CDS             1675..1971
                     /locus_tag="PKV97_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07646.1"
                     /translation="MSAHSSASRRRVAANAGPEYDTHPELALAAVLQLLSRFPARRSP
                     ALAQAIAGHFRVIGSDQRLSACVRDCAARLVAEWEAYAMLGDAGDGGHDPQALH"
BASE COUNT          303 a          671 c          727 g          321 t
ORIGIN      
        1 cctcggcggc gccgccgcgc tcgcgcgcga gccgcgccgg gagcgagtcg cggccgatgg
       61 gcgcggtgag ctcggcgagc gcggcgcgca cggtctcact gttcgcttgc gccgcgagcc
      121 aggcctcgac ctcggggtcg cggccgatgg cgaggatggc cagcccgcgt gcggccaggc
      181 gcggatgcgg gcattgcgcg aggatgagct gcttgacgcg ggcgatgtcg cgctcgagca
      241 ccgggtcgta gcgcagcagt ggtgccggca ccgccgcacg ctgcagcgct tcggtgagcg
      301 cggaaactcc ttccccgccg gtcgccaccg tcgccaccac cggcacgccg agtgcctccg
      361 acaaggcctc ggcatccact gtgaccccgc gcgccttggc ctcgtcggtc atgttgagcg
      421 ccatcaccat cggcaccccg agttcggcca gcagcgcggt gaggttgagc gtgcggcgca
      481 ggttcttggc gtcgccgacc tggatcagcg tgcgcatgtc ctcatggagc agcgcgcgca
      541 tggtggcgcg ctcgtcgtcg ctgcgcgatg gcagggcgag cacgcccggg gtgtcgagca
      601 gcgaggcctc gcggtcgaag cgcggcgtgg cgcgcgagac ctcgaccgtg gtgccggggt
      661 aattggacac cgtgacgtag gtgccggtga ggcggtggaa cagcaccgac ttgccgacgt
      721 tggggtggcc gacgagggcc agggtgcgca gcgcggcggc agcggcggag gagggcgaag
      781 tggcgcagtc catgaactcg ggtgtccgga gggcggagta agcctagaac gataatgggt
      841 cgcattatga cttttactga tctagctcaa aggtcgtgcg cccggcgtgt attcgatgcg
      901 gtccggaccg caggacagag aggcttgaca gtgattcgga ttttaacgat attgcgaatc
      961 attcgcaaag acgatattca accgaccctg tctcgacttc gaggctgtcc tgctggcgct
     1021 cttcgtgcgt cggcgcagca agaatccctg aatgaaggag aacccattgt cccgatacac
     1081 gggcccccgc ctgaaagtga tgcgtgccct cggcgccgag ctgcccggcc tgtcgcgcaa
     1141 gccgctgggt gagcgcaact atccccctgg ccagcacggc cggcgcccga ggcgcaagtc
     1201 cggcttcggc gtgcagctga tcgagaagca gaagctgcgc ttcaactatg gtctcggcga
     1261 gaagcagatc cagcgcctgt tccgcgaggc caagcgcgac cgcggcccga ccggcgacaa
     1321 gctgctggaa ttgctcgagc gtcgcctcga caacttcgtg ttccgcgccg gcttcgcgcc
     1381 caccgcggtg gcggcgcggc agctcgtgcg ccacaagcac gtactgctca acggacgggc
     1441 ggtgaacatc ccctccatcc gcatccggcc gggcgaccgc atccagctca cggaacgtgc
     1501 gcggtgcatc ccggtggtgg tcgaggcgct cgccgagcct gcgctgaccc gccccgaatg
     1561 gctcgtcttc gacaaggcgg cgttcgtggc gacgatgacg cgcaccccgt cgcccgacga
     1621 ggtgcccttc ccggtcgacg tccagcaggt cgtcgagtac tacgcggtgc ggctgtgagc
     1681 gcgcattcat ccgccagccg gcgaagggtt gccgcgaacg cggggccgga gtacgacacc
     1741 catcccgagc tcgcgctggc ggcggtgctg cagctgctgt cgcgcttccc ggcgcggcgc
     1801 tcgcccgcgc tggcgcaggc gatcgccggt cacttccggg tgatcggcag cgatcagcgc
     1861 ctttccgctt gtgtgcgcga ctgcgccgcc cggctggtgg cggagtggga ggcctatgca
     1921 atgctgggcg atgcgggcga tggcggccac gatccgcagg cactgcactg agcagtccgg
     1981 ttcgatggtg tccgtctttt ccattccacg aggagaagtc ca
//
LOCUS       DAOQNN010000537         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_47607_length_2015_cov_7.738265, whole genome shotgun sequence.
ACCESSION   DAOQNN010000537 DAOQNN010000000
VERSION     DAOQNN010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47607_length_2015_cov_7.738265"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PKV97_17060"
     CDS             <1..896
                     /locus_tag="PKV97_17060"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNB07647.1"
                     /translation="CSMGPPTSGGIALLQVLGILRQFDLAPMHPNGAQAMHLLAEASR
                     LAFADRNRYVADEDFARVPVAGLLDEAYLRARAGQIRADRSLGEAQPGKLSDPLVLAL
                     LPPLASQRQFEPASTSHLIIVDSAGDIVSMTSSIERAFGSQLMVKGFMLNSELTDFSF
                     LPASEGRLVANRVEPGKRPRSSMTPVIAFDKDGNPAFAVGSPGGSSIIGYVAQAVVEL
                     ADWGRPPGQAVAAPHVLNRNGATELEAGTAATDLRAALEGLGHQVQESALQSGLAIVR
                     FVDGRMVGAADPRRDGVALGE"
     gene            934..>2015
                     /locus_tag="PKV97_17065"
     CDS             934..>2015
                     /locus_tag="PKV97_17065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNB07648.1"
                     /translation="MTQDRASVKLMQGGDRRALTGHPWIFSNEVAMDAATKALPPGEV
                     VTVERADGRALGVGLFNPRSLIAIRLLTRDASRAIDEDFLAKRLKRAAKLRDRMIGVP
                     HYRLIHAEADGLPGCVVDRFGDVLVVEPNSAGMERLSEQLVAALDRMLRPKAIVVSGD
                     GPARALEGLEPVHRLAKGGLAGPIEVLEHGAHFLADPSAGQKTGWFYDQRDNRALAAR
                     FARDADVLDLYAYSGGFGIQAARHGAKSVLCVDRSAPAISLLRQAAERNEAAARVTTE
                     VAEAFEFLEMAAATHRTFDLVIADPPAFVKAKKDFHQGGRAYRKLARMCASRVRSGGF
                     LFLASCSHHMPAEEFQAQVARGLHEAG"
BASE COUNT          283 a          735 c          708 g          289 t
ORIGIN      
        1 tctgcagcat ggggccgccg acctcgggcg gcatcgcgct gctgcaggtg ctcggcatcc
       61 tgcgccagtt cgacctcgcg ccgatgcacc ccaatggcgc gcaggcgatg catttgctgg
      121 ccgaggccag ccgcctcgcc ttcgccgacc gcaatcgcta tgtcgccgac gaggatttcg
      181 cccgcgtgcc ggtcgccggc ctgctcgacg aggcttacct gcgcgcgcgc gccgggcaga
      241 tccgcgccga ccgcagcctg ggcgaggcgc agccgggaaa actcagcgac ccgctggtcc
      301 tcgccctgct gccgcccttg gcgagccagc ggcagttcga gcccgcctcc acctcgcacc
      361 tgatcatcgt cgacagcgcc ggcgacatcg tcagcatgac cagctccatc gaacgcgcct
      421 tcgggtcgca gctcatggtg aagggcttca tgctgaacag cgagctcacc gatttctcct
      481 ttctcccggc ctccgagggg cgcctggtcg ccaaccgcgt cgagcccggc aagcggccgc
      541 gcagctcgat gacgccggtc atcgccttcg acaaggacgg caatcccgcc ttcgcggtcg
      601 gctcgccggg cgggtccagc atcatcggct acgtggcgca ggcggtggtc gaactggccg
      661 attggggccg tccgccgggc caggcggtgg cggcgcccca tgtgctgaac cgcaacggcg
      721 ccaccgaact cgaggccggc accgcggcga ccgatctgcg cgccgcgctc gaaggcctcg
      781 gccaccaggt gcaggagagc gcgttgcaga gcggcctcgc catcgtgcgg ttcgtcgacg
      841 gtcgcatggt gggcgccgcc gatccgcgcc gcgacggcgt cgcgctgggc gaatgacgcg
      901 cggcagtcac gcgcaaggaa acaggatacc gacatgaccc aggatcgcgc ctcggtgaag
      961 ctgatgcagg gtggcgaccg ccgcgccctg accggccatc cgtggatctt ctccaatgaa
     1021 gtggcgatgg atgccgcgac caaggcgctg ccgccgggcg aggtcgtcac cgtggagcgc
     1081 gccgacggcc gcgcgctcgg cgtcgggttg ttcaatccac gctcgctcat cgccatccgc
     1141 ctgctgaccc gcgacgcgtc gcgcgcgatc gacgaggatt tcctggcgaa gcgcctgaag
     1201 cgcgcggcga agctgcgcga ccgcatgatc ggcgtgccgc actaccgcct gatccacgcc
     1261 gaggccgacg gcctgcccgg ctgcgtcgtc gaccgcttcg gcgacgtgct ggtggtcgag
     1321 ccgaactcgg ccggcatgga gcggctgtcg gagcagctgg tggcggcgct cgacaggatg
     1381 ctgcggccga aggccatcgt ggtctccggc gacgggccgg cgcgggcgct ggagggactc
     1441 gagccggttc atcggctggc caagggcggc ctcgccgggc cgatcgaggt gctggagcac
     1501 ggcgcgcatt tcctcgccga tcccagcgcc gggcagaaga ccggctggtt ctacgaccag
     1561 cgcgacaacc gggcactggc ggcgcgcttc gcccgcgacg ccgacgtgct cgacctctac
     1621 gcctatagcg gcggcttcgg catccaggcg gcgcgccacg gcgccaagtc ggtgctctgc
     1681 gtcgaccgct cggcgcccgc catctccctg ctgcgtcagg cggcggagcg caacgaggcg
     1741 gccgcgcggg tgacgaccga ggtcgccgaa gccttcgagt tcctggaaat ggccgcggcc
     1801 acccatcgca ccttcgacct ggtgatcgcc gacccgccgg ccttcgtcaa agccaagaag
     1861 gatttccacc agggcggccg cgcctatcgc aagctggcgc ggatgtgcgc gtcacgggtg
     1921 cgcagcggcg gcttcctgtt cctcgcctcc tgctcgcatc acatgccggc ggaggagttc
     1981 caggcccagg tggcgcgcgg cctgcacgag gccgg
//
LOCUS       DAOQNN010000538         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_47775_length_2011_cov_11.000511, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000538 DAOQNN010000000
VERSION     DAOQNN010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47775_length_2011_cov_11.000511"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PKV97_17070"
     CDS             complement(<1..344)
                     /locus_tag="PKV97_17070"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNB07649.1"
                     /translation="MVPHLTTALTGPLLELERQFLDNACDIEKWFRTQWQDHMPPFYG
                     STDLRNSGFKLAPVDLNLFPGGFNNLNDVFMPLCVQAAQAAIERICPDASKLLLIPEN
                     HTRNQFYLQNVA"
     gene            complement(464..>2011)
                     /locus_tag="PKV97_17075"
     CDS             complement(464..>2011)
                     /locus_tag="PKV97_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNB07650.1"
                     /translation="IFAAALFFGDSMITPAISVLSAVEGIGIVAPGLEAWVVPVTVLI
                     LTVLFAIQRRGTGVVGLFFGPVMVVWFAVLGALGMVEIVRYPEILAALSPLHAVAFVV
                     DHPALAFLALGAVVLAVTGGEALYTDMGHFGPAPIRLGWFGFVLPALVVNYFGQGALL
                     LHEPAAIESPFYHLGPSWAMIPLLVLATLATVIASQAVISGAFSVARQAVQMGLLPRM
                     LIVHTSGHEQGQIYVPFTNWTLYLAVVALVVGFQNSSNLAAAYGIAVTGTMLIDTVLL
                     GFVMVLIWRWRPLLAGLCAGTLLLVDLAFFSANAIKIPQGGWFPIAMALVSFTVLTTW
                     RQGRRLVQQEIAAQGLPLNAFIDMVGEVHRVQGTAVFMTSTKDGVPAALLHNLKHNQV
                     LHERVALVTVETADTPYVSELDRIYLHRLRNGFMRVIVRYGFMEDPDIPSALKHCSRF
                     GESFEMMETTFFVSRETVIPRLSRRLFSDWRARLFAFMSKNATSATDFFRIPNNRVVE
                     LGTQLVI"
BASE COUNT          377 a          653 c          693 g          288 t
ORIGIN      
        1 ttggcgacgt tctgcaggta gaactggttg cgggtgtggt tctcggggat cagcaacagc
       61 ttgctggcat ccgggcagat gcgctcgatc gccgcctgcg ccgcctgcac gcacagcggc
      121 atgaagacgt cgttgaggtt gttgaagccg cccgggaaca ggttcaggtc gaccggcgcg
      181 agcttgaagc ccgagttgcg caggtcggtg gaaccgtaga aaggcggcat gtggtcctgc
      241 cactgggtac ggaaccactt ttcgatgtcg caggcgttgt cgaggaactg gcgctccagc
      301 tccagcaggg ggccggtaag cgcggtggtg aggtgcggaa ccatggattg ggtgtctctc
      361 gtgagggggc cgccacccgg cgggcggcag ccgatgcttt ggccggaatg ttacaccaaa
      421 gcccgggggc gccttccggc cgccccgatg gcgcgtggcc gcgtcagatc accaactgcg
      481 tgccgagttc gaccacccgg ttgttgggga tgcggaagaa gtcggtcgcg ctggtggcgt
      541 tcttggacat gaaggcgaac agccgcgccc gccagtcgga aaacagcctg cggctgagcc
      601 tggggatcac cgtctcgcgc gagacgaaga aggtggtctc catcatctcg aacgattcgc
      661 cgaagcggct gcagtgcttc agcgcgctcg ggatgtcggg atcctccatg aagccgtagc
      721 gcacgatcac gcgcatgaag ccgttcctga ggcgatgcag gtagatgcgg tcgagctcgc
      781 tcacgtaggg ggtgtcggcc gtctccacgg tcaccagcgc gacgcgctcg tgcaggacct
      841 ggttgtgctt gaggttgtgc agcagcgcgg cggggacgcc atccttggtg ctggtcatga
      901 acaccgccgt gccctgcacc cgatgcacct ccccgaccat gtcgatgaac gcgttcagcg
      961 gcaggccctg ggcggcgatc tcctgctgca ccaggcgacg cccctggcgc caggtggtga
     1021 gcacggtgaa cgagaccagc gccatggcga tcgggaacca gccgccctgg ggaatcttga
     1081 tcgcgttggc gctgaagaag gcgaggtcca ccagcagcag cgtccccgcg cacagtccgg
     1141 ccagcagcgg acgccagcgc cagatcagca ccatcacgaa gccgagcagc accgtgtcga
     1201 tcagcatcgt gccggtcacc gcgatgccgt atgccgcagc gaggttcgac gagttctgga
     1261 agccgaccac gagtgcgacc accgccaggt aaagcgtcca gttggtgaag gggacgtaga
     1321 tctgcccctg ctcgtgcccc gaggtatgga cgatcagcat gcgcggaagc agccccatct
     1381 gcaccgcctg gcgggccacc gagaaggcgc ccgagatcac cgcctgcgag gcgatgaccg
     1441 tggccagggt ggcgagcacc agcagcggga tcatcgccca cgacggcccg aggtggtaga
     1501 atgggctttc gatcgccgcc ggctcgtgca gcagcagcgc cccctggccg aagtagttca
     1561 ccaccagggc cggcaacacg aagccgaacc accccagccg gatcggggcc gggccgaaat
     1621 gtcccatgtc ggtgtagagc gcctccccgc cggtcaccgc gagcacgacc gcgcccagcg
     1681 cgaggaaggc gagcgccgga tgatcgacga cgaaggcgac cgcatggagc ggactgaggg
     1741 ccgcgaggat ctccggataa cgcacgatct cgaccatgcc gagcgcaccc agcacggcaa
     1801 accagaccac catgaccggg ccgaagaaga gccccaccac cccggtgccg cggcgctgga
     1861 tcgcgaacag cacggtgagg atgagcaccg tcaccggcac cacccaggcc tcgagccccg
     1921 gcgccacgat gccgattccc tccaccgccg acagcaccga gatcgccggc gtgatcatgc
     1981 tgtcgccgaa gaacaaggcg gcggcgaaga t
//
LOCUS       DAOQNN010000539         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_47776_length_2011_cov_10.690695, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000539 DAOQNN010000000
VERSION     DAOQNN010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47776_length_2011_cov_10.690695"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKV97_17080"
     CDS             <1..420
                     /locus_tag="PKV97_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07651.1"
                     /translation="WSGEEAPEVSLRLAVADLPRLLVDGLETAMRHVRIEGNAEFAEA
                     LGFVFRHLRWDAEEDLSRLFGDIAAHRLVEGGRKVVDEGRRSLERASGNVAEYLTEES
                     TLLVPRKALPAFAEEVVALRDAVARLDKRVARLEKRR"
     gene            complement(576..1001)
                     /locus_tag="PKV97_17085"
     CDS             complement(576..1001)
                     /locus_tag="PKV97_17085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNB07652.1"
                     /translation="MAMTVHVDIVSAEEQIFSGLAEFVALPGEAGELGILPGHMPLMT
                     RIKPGAVRVRAQNATEEELIFVAGGLLEVQPGLVTVLADTAIRGKDLDEAKALEAKRK
                     AEEAMANQTARLDYAKAQAELMEAVAQLAAIQRLRKRGH"
     gene            complement(1056..>2011)
                     /gene="atpD"
                     /locus_tag="PKV97_17090"
     CDS             complement(1056..>2011)
                     /gene="atpD"
                     /locus_tag="PKV97_17090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNB07653.1"
                     /translation="KVGLFGGAGVGKTVNMMELINNIAKQHSGLSVFAGVGERTREGN
                     DFYHEMKDSNVLDKVAMVFGQMNEPPGNRLRVALTGLTMAERFRDDGRDILLFIDNIY
                     RFTLAGTEVSALLGRMPSAVGYQPTLAEEMGRLQERITSTKVGSITSIQAVYVPADDL
                     TDPSPATTFLHLDSTVVLSRDIAALGIYPAVDPLDSTSRQLDPLVVGEEHYNVARQVQ
                     MTLQKYKELRDIIAILGMDELSPEDKLAVARARKIQRFLSQPFHVAEVFTGSPGKYVS
                     LKDTIKGFKMIVSGELDSLPEQAFYMVGGIDEAIEKAKKLQ"
BASE COUNT          334 a          656 c          674 g          347 t
ORIGIN      
        1 tggagcggtg aggaagcgcc cgaggtgagc ctgcgcctcg cggtggccga ccttccgcga
       61 ctgctggtgg atgggctgga gaccgcgatg cgccacgtcc gcatcgaggg caacgccgag
      121 ttcgccgagg cgctcggctt cgtcttccgc cacctgcgct gggatgcaga ggaggacctg
      181 tcacgcctgt tcggcgacat cgccgcccac cgcctggtgg aaggcgggcg caaggtggtg
      241 gacgagggcc gccgcagcct ggagcgcgcg agcggcaacg tggccgaata cctcacggag
      301 gaatccaccc tgctcgtccc gcgcaaggcg ctgcccgcat tcgccgaaga ggtggtggcg
      361 ctgcgcgacg cggtggcgcg cctggacaag cgcgtggcga ggctggaaaa gcggcgctga
      421 cgagcgcgcg gcgtggcacc cgatccgcta aagcgggagc cccgcatcgc gcccgctcgt
      481 gtgcgcccgc gccggggcgt gcacgaaaaa aaagcagctc ggcgagagct gctttcggga
      541 tgtcgaacgc accggcgggc cggcgcgctc cggtctcagt gaccgcgctt gcgcagacgc
      601 tggatcgcgg cgagctgcgc caccgcttcc atgagttcgg cctgggcctt ggcatagtcc
      661 agacgtgcgg tctggttcgc catggcttcc tcggccttgc gcttggcttc gagcgccttc
      721 gcctcgtcga ggtccttgcc gcggatcgcg gtgtcggcga gcaccgtcac cagacccggt
      781 tgaacctcca gcaggcctcc cgcgacgaag atcagctctt cctcggtcgc gttttgggca
      841 cgcacgcgca cggcacccgg cttgatccgg gtcatcagcg gcatgtgacc gggcaggatg
      901 cccagctcgc ccgcttcgcc cggcaatgcg acaaactcgg ccagcccgga gaagatctgc
      961 tcttccgcgc tgacgatgtc gacatgaacc gtcatagcca ttgtgtttcc ccttgatgtc
     1021 ccgcgcgccg cttgcacgac gcgcgggcga cctcgttact ggagcttctt cgccttctcg
     1081 atggcctcgt cgatgccgcc gaccatgtag aaggcctgtt ccggcaggct gtcgagctca
     1141 ccggaaacga tcatcttgaa gcccttgatc gtgtccttca gcgagacgta cttgcccggc
     1201 gagccggtaa acacttccgc gacgtggaag ggctgcgaca ggaagcgctg gatcttgcgc
     1261 gcgcgagcca cggcgagctt gtcttccggc gacagttcgt ccatgcccag aatcgcgatg
     1321 atgtcgcgca gttccttgta cttctgcagc gtcatctgaa cctgacgcgc cacgttgtag
     1381 tgctcttcgc ccacgaccag cggatcgagc tggcgcgagg tggagtcgag cggatcgacg
     1441 gccgggtaga tacccagggc agcgatgtca cgcgacagca cgacggtgga gtcgaggtgc
     1501 aggaaggtgg tggcaggcga cgggtcggtc aagtcgtccg caggcacata cacggcctgg
     1561 atcgaggtga tcgaaccgac cttggtggag gtgatgcgct cctgcaggcg gcccatttct
     1621 tcggccagcg tcggctggta gcccacggcg gaaggcatac ggcccagcag cgcggacact
     1681 tcggtaccgg ccagcgtgaa gcgataaatg ttgtcgatga acagcaggat gtcacggccg
     1741 tcgtcgcgga aacgctcggc catggtcagg ccggtcagcg ccacgcgcag acggttgccc
     1801 gggggctcgt tcatctgacc gaacaccatc gcgaccttgt cgagaacgtt ggagtccttc
     1861 atctcgtggt agaagtcgtt accctcacga gtacgctcac ccacgccagc gaacaccgac
     1921 agaccggagt gctgcttcgc gatgttgttg atcagctcca tcatgttcac ggtcttgccg
     1981 acgccggcgc caccgaacag acccaccttg c
//
LOCUS       DAOQNN010000540         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_47871_length_2009_cov_5.778403, whole genome shotgun sequence.
ACCESSION   DAOQNN010000540 DAOQNN010000000
VERSION     DAOQNN010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47871_length_2009_cov_5.778403"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1242)
                     /locus_tag="PKV97_17095"
     CDS             complement(214..1242)
                     /locus_tag="PKV97_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HNB07654.1"
                     /translation="MSRPAACALANHRRPCAHALQSLLAGLLVALCALLFTALPAHAE
                     DFAGDFNYRLQPRELAPGAWVVVGLKEDFSFANGGNIVNTGFIVGREGVIVIDTGSSK
                     RYGEQMLAAIRRVTPLPIVLTVNTHHHPDHFLGNQAFPRATLAALPETIAALRSEGEG
                     FNANMYRLNGDWMRDTEVVVPERALAAGRQRIGGRDVELVALGGHTVADLVVIDHDSG
                     TVYAADLLFNGRAPTTPHADLLRWLAALDTLEGLPARRWVPGHGEPATDLAPLRQTRD
                     YLGWLAQAIRTGAESGQDMTELFQTPIPQRFAGLALVEAEFRRSVVHLFPAAEQAVLE
                     AARAQAPR"
     gene            complement(1239..>2009)
                     /locus_tag="PKV97_17100"
     CDS             complement(1239..>2009)
                     /locus_tag="PKV97_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HNB07655.1"
                     /translation="PAVAEDSLNVPVNVDASAPSPPAAHTDPLDSPRWEDMRKSFFAD
                     VAVVFDERVKVSGPAVAEDSLNVPVNVDASALPGVEELIVFADFNPIVKALRFEPGAA
                     QPRLGFRIKLQQSSPVRAAARTADGVWHVGGAWITATGGGCTLPSVGSGSPEWQERLN
                     EVNGKLWPRVDGGERLRMRIIHPMDTGLAAGIPAFHIEDIVIADAEGRTLSRIQPLEP
                     VSENPSFTLDLRAAAPGARLHVAGRDNNGNRIDAWVTR"
BASE COUNT          266 a          672 c          792 g          279 t
ORIGIN      
        1 ggccgctgcc aacggcgacg aggaggccgc gcgcaggctg tccgcgctgc ccggaccgac
       61 gccggccgtg cagtgatgag gccgcgtgcc cgcgcacgcc cgcaggcggg acatgcgcac
      121 gagcgcgtgg acgcccgtct cgctgcactc tcggtttcct gttcgcggca cctgcgtcgc
      181 gagcgctgca gctgcgatgt gatcgtgctg cgctcagcgc ggcgcctgcg cgcgcgcggc
      241 ttcgagcacg gcctgctctg cggccgggaa gaggtgcacc accgagcggc ggaactccgc
      301 ctccaccagc gccaggccgg cgaagcgctg cgggatcggc gtctggaaga gctcggtcat
      361 gtcctggccc gactcggcgc cggtgcggat ggcctgcgcc agccagccga gatagtcgcg
      421 ggtctggcgc agcggggcga ggtcggtcgc gggctcgccg tggccgggca cccagcgccg
      481 cgcgggcagg ccttccagcg tgtcgagcgc ggccagccag cgcaagaggt ccgcgtgcgg
      541 cgtggtgggc gcgcggccgt tgaagagcag gtcggcggcg tacacggtgc cgctgtcgtg
      601 gtcgatcacc accaggtcgg cgacggtgtg gccgccgagc gcgaccagct ccacgtcgcg
      661 cccgccgatg cgctggcgcc cggcggcgag tgcgcgctcg gggacgacga cctcggtgtc
      721 gcgcatccag tcgccgttga ggcggtacat gttggcgttg aagccttcgc cctcgctgcg
      781 gagcgcggcg atggtctccg gtagcgcggc gagcgtcgcg cgcgggaagg cctggttgcc
      841 gaggaagtgg tcggggtggt ggtgggtgtt caccgtcagc acgatcggca agggcgtgac
      901 gcggcggatg gcggcgagca tctgctcgcc gtagcgcttc gacgagccgg tgtcgatgac
      961 gatcaccccc tcgcggccga cgatgaagcc ggtattgacg atgttgccgc cgttggcgaa
     1021 ggagaaatcc tccttcaggc cgacgacgac ccaggcgccg ggggcgagct cgcgcggctg
     1081 caggcggtag ttgaagtccc cggcgaagtc ctcggcgtgc gccggcaggg cggtgaagag
     1141 cagggcgcac agcgcgacca gcagccccgc gagcagggac tgcagggcgt gggcgcaagg
     1201 gcggcggtgg ttcgccaagg cgcaagcggc gggtcggctc atcgggtcac ccatgcgtcg
     1261 atgcggttgc cgttgttgtc gcgcccggcc acgtgcaggc gcgcgccggg cgcggcggca
     1321 cgcaggtcga gcgtgaagct ggggttctcg gagaccggct cgaggggctg gatgcggctt
     1381 agcgtgcggc cttcggcgtc ggcgatcacg atgtcctcga tgtggaaggc ggggatgccg
     1441 gcggccagcc cggtgtccat cggatggatg atgcgcatgc gcaggcgctc gccaccgtcg
     1501 acccgcggcc acagcttgcc gttgacctcg ttgaggcgct cctgccattc cggtgagccc
     1561 gagcccaccg agggcagcgt gcagccgccg ccggtggccg tgatccaggc gccgccgacg
     1621 tgccacaccc cgtcggccgt gcgcgcggcg gcgcgcaccg gcgaggactg ctgcagcttg
     1681 atgcggaagc ccaggcgcgg ctgggcggcg ccgggctcga agcgcagcgc cttgacgatc
     1741 gggttgaagt cggcgaacac gatcagctcc tccaccccgg ggagggcgct cgcatcgaca
     1801 ttgaccggga cgttgagcga atcctccgcg accgccgggc cgctcacctt gacgcgctcg
     1861 tcgaacacca ccgcgacgtc cgcgaagaag ctcttgcgca tgtcttccca gcgtggcgag
     1921 tcgagcgggt cggtgtgcgc ggcggggggg gagggggcgc tcgcatcgac attgaccggg
     1981 acgttgagcg aatcctccgc gaccgccgg
//
LOCUS       DAOQNN010000541         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_48084_length_2004_cov_10.766547, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000541 DAOQNN010000000
VERSION     DAOQNN010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48084_length_2004_cov_10.766547"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2004
                     /locus_tag="PKV97_17105"
     CDS             <1..>2004
                     /locus_tag="PKV97_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNB07656.1"
                     /translation="AYGIPLMKVAQVIRDSNRDVGGRVVEMAETEYMVRGKGYLRGKA
                     DIERLVVKADKGTPVLVRDIARVELAPDERRGLTELNGDGEVVAGIAMARYGQNALEV
                     IHNLKDKIAEIGPGLPEGVSVQTVYDRSELIHRAIETLKGTLIEESIIVALVCVVFLL
                     HVRSALVAILMLPVGVLMAFIAMRLLGMNSNLMSLGGIAIAIGAMIDAAIVMIENAHK
                     HLERLPEGHTTNERFEAMLAACKEVGPALFFSLLIITVSFLPVFTLEGQEGRLFSPLA
                     YTKTFSMAGAAFLSVTLVPVLMLLFIRGKIMPEAKNPVNRVLIWLYRPIIAAVMRWKK
                     LTIVLAVLALGVSIYPASRLGSEFMPTLNEGTLLYMPASLPGMSITKAAELMQTQDKI
                     IKSFPEVESVFGKAGRANTATDPAPTEMFETVINLKPQSEWRPGMTTDKLISEMDKAL
                     QFPGVANAWTMPIKARTDMLSTGIRTPIGIKVFGKDLAEMEKLAKEIEAVVKTVPGTT
                     SAFAERITGGFYLNIEPDREQLARYGLAVGDLQDVIGSALGGEMVTTTVEGRERFGVT
                     VRYPRELRSDPQQIASQVLVPTMGGTMVPLGQLAKVSVAKGTPGIRTENALLSAYIFV
                     DIRDRDIGGYVADAKKAVAAQIKFPPGYYVTWSGQFEFMERAI"
BASE COUNT          363 a          641 c          654 g          346 t
ORIGIN      
        1 gcgcctacgg cattccgctg atgaaggtcg cccaggtgat ccgcgactcc aaccgggacg
       61 tcggcggccg cgtggtggag atggccgaga ccgagtacat ggtgcgcggc aagggctacc
      121 tgcgcggcaa ggcggacatt gaacggctgg tggtgaaggc tgacaagggc acgccggtgc
      181 tggtccgcga catcgcccgc gtcgagctgg cgccggacga acgccgcggc ctcaccgagc
      241 tgaacggcga cggcgaggtg gtggccggca tcgccatggc ccgctacgga cagaacgcgc
      301 tggaggtcat ccacaacctg aaggacaaga tcgccgagat cgggcccggc ctgccggaag
      361 gcgtgtcggt gcagaccgtc tatgaccgct ccgagctgat ccaccgggcc atcgagaccc
      421 tgaagggcac gctgatcgag gagtcgatca tcgtggcgct agtctgcgtg gtcttcctgc
      481 tccatgtgcg cagcgcgctg gtggcgatcc tgatgttgcc ggtgggcgtg ctgatggcct
      541 tcatcgcgat gcgcctgctg ggcatgaact ccaacctgat gagcctgggt ggcatcgcca
      601 ttgcgatcgg cgcgatgatc gacgccgcca tcgtgatgat cgagaacgcc cacaagcacc
      661 tggaacgcct gccggaaggg cacaccacca acgagcgctt cgaagccatg ctggccgcct
      721 gcaaggaggt cggcccggcg ctgttcttct cgctgctcat catcaccgtc tccttcctgc
      781 cggtgttcac gctggaaggc caggaaggcc gcctgttctc gccgctggct tacaccaaga
      841 ccttctcgat ggccggcgcc gcgttcctgt ccgtcacctt ggtgccggtg ctgatgctgc
      901 tgttcatccg cggcaagatc atgccggaag ccaagaaccc ggtgaaccgg gtcctgatct
      961 ggctgtaccg gccgatcatc gccgcggtga tgcgctggaa gaagttgacc atcgtgctgg
     1021 ccgtgctggc cttgggcgtg tcgatctatc cagcttcgcg tctcggctcg gagttcatgc
     1081 cgaccttgaa cgaaggcacc ttgctctaca tgccagcctc tctgccgggc atgtccatca
     1141 ccaaggctgc cgagctcatg cagacccagg acaagatcat caagagcttc cccgaggtcg
     1201 agtcggtgtt cggcaaggct ggccgcgcca acacggcgac cgatccggcg ccgacggaga
     1261 tgttcgagac cgtcatcaac ctcaagccgc aatccgaatg gcggccgggc atgaccaccg
     1321 acaagctgat ctcagaaatg gacaaggccc tgcagttccc cggcgtcgcc aatgcctgga
     1381 cgatgccgat caaggcccgc accgacatgc tgtccaccgg catccgcacg ccgatcggca
     1441 tcaaggtctt cggcaaggat ctcgctgaaa tggagaagct ggccaaagag atcgaggccg
     1501 tcgtgaagac cgtgcccggc accaccagcg ccttcgccga gcgcatcact gggggcttct
     1561 acctcaacat cgagcccgac cgggaacagc tggcccgcta cggtctcgcc gtcggcgacc
     1621 tgcaggatgt gatcggtagt gcgctcggcg gcgagatggt caccaccacc gtcgaagggc
     1681 gcgagcgctt tggcgtgacc gtacgctacc cgcgcgagct gcgctccgat ccccagcaga
     1741 tcgccagcca ggtgctggtg ccgacgatgg ggggcacgat ggtgccgctg gggcagttgg
     1801 cgaaggtctc ggtcgcaaaa ggtacgccgg gtattcgcac tgagaatgcc ttgttatcgg
     1861 cctatatctt cgtggatatc cgggaccggg atatcggtgg ctacgtggcc gacgcgaaga
     1921 aggctgtcgc cgcgcaaatc aagttcccac cgggctacta cgtgacctgg agcggccagt
     1981 tcgagttcat ggagcgcgcg atcg
//
LOCUS       DAOQNN010000542         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_48086_length_2004_cov_8.331965, whole genome shotgun sequence.
ACCESSION   DAOQNN010000542 DAOQNN010000000
VERSION     DAOQNN010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48086_length_2004_cov_8.331965"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PKV97_17110"
     CDS             <1..962
                     /locus_tag="PKV97_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HNB07657.1"
                     /translation="GCTFCSITEHEGRIIQSRSHESIIHEIEEIRDKSPDFKGHITDL
                     GGPTANMYRMACKSKAIEENCRRLSCVYPGICENLDTDHSSLVELYRKARAVPGIKRI
                     TIGSGLRYDLAVRSPEYVKELVTHHVSGLLKIAPEHTEENTLSKMMKPGIGAYEEFAR
                     MFEKYSKLAGKKQHLVPYFIAAHPGTTDEDMLNLALWLKAHDFRLDQVQTFLPTPMAL
                     ATTMYHSRKNPLKKVAADSEVVETARAGRIRKLHKAFLRWHDPENWPLLREALIEMGR
                     AELIGNGPHCLVPRQQPARLVAAKAGGGRKPGGCAKSRARRGA"
     gene            complement(959..>2004)
                     /locus_tag="PKV97_17115"
     CDS             complement(959..>2004)
                     /locus_tag="PKV97_17115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB07658.1"
                     /translation="EADHASAEPALILYGGSALSLMLFAVMHSNARHRRRMRAAASHL
                     AHSRDEFRTLVENVPGVVFRCQIEPPWRVMHISRGITALTGATPEDFMSGRLSFGDFI
                     LHEDVPTIVAAIESARISHTNYEVEYRMRGRDRRVHWVSERGRVAVDADDEPLRLDGV
                     IIDITERKAAEEAIRNLAFVDTLTQLPNRRFLLDRLRQGLANARRHGRHGALLFIDID
                     HFKRVNDTYGHEAGDQLLCEIAARLRQVVREGDTVARLGGDEFVVMLEDLGDATAETG
                     EKAALIAGNILAALNEPYAIGKVRHHSTPSIGIACFDGGEQRAEELLRRADEAMYRAK
                     AGGRNRLETLNAS"
BASE COUNT          327 a          670 c          690 g          317 t
ORIGIN      
        1 gcggctgcac cttctgctcg atcaccgagc acgagggccg catcatccag agccgttcgc
       61 acgaatccat cattcacgag atcgaggaga tccgcgacaa gtcgccggac ttcaagggcc
      121 acatcaccga cctcggcggg cccaccgcca acatgtaccg catggcgtgc aagagcaagg
      181 cgatcgagga gaactgccgg cgactctcgt gcgtgtatcc gggcatctgc gagaacctcg
      241 acaccgacca ttcctcgctc gtcgagctct accgcaaggc gcgcgcggtg ccagggatca
      301 agcgcatcac catcggctcg ggcctgcgct acgacctcgc ggtgcgctcg ccggaatacg
      361 tgaaggagct cgtcacccac cacgtcagcg gcctgctcaa gatcgcgccc gagcacaccg
      421 aggagaacac gctctccaag atgatgaagc cgggcatcgg cgcctacgag gagttcgcgc
      481 gcatgttcga gaagtactcg aagctcgcgg gcaagaagca gcacctggtg ccgtacttca
      541 tcgccgccca tccgggcacc accgacgagg acatgctcaa cctggcgctg tggctcaagg
      601 cgcacgactt ccgcctcgac caggtgcaga ccttcctgcc cacgccgatg gcgctggcga
      661 ccacgatgta ccactcgcgc aagaacccgc tgaagaaggt cgcggccgat tccgaggtgg
      721 tggagaccgc gcgcgccggc cgcatccgca agctgcacaa ggccttcctg cgctggcacg
      781 acccggagaa ctggccgttg ctgcgcgagg cgctgatcga gatggggcgc gccgagctga
      841 tcggcaacgg cccgcactgc ctggtgccgc gccagcagcc ggcacggctg gtggcggcga
      901 aggccggggg cgggcgcaag ccgggtggct gcgcgaaatc gcgggcgagg cgcggggcct
      961 aggaggcgtt cagcgtctcg agccggttgc gtccgccggc cttggcgcgg tacatcgcct
     1021 cgtcggcccg ccgcagcagt tcttcggcgc gctgctcgcc gccgtcgaag caggcgatgc
     1081 cgatgctggg cgtgctgtga tgacgcactt tcccgattgc atatggctcg ttgagtgcgg
     1141 cgaggatgtt gccggcgatc agggctgcct tttcgcccgt ctcggctgtg gcgtcgccca
     1201 gatcctccag catcaccacg aactcgtctc caccgaggcg cgccacggtg tcgccctcgc
     1261 gcaccacctg gcgcaggcgc gccgcgatct cgcacagcag ctggtcgccg gcctcgtggc
     1321 cgtaggtgtc gtttacgcgc ttgaagtggt cgatatcgat gaacagcagc gccccgtggc
     1381 ggccgtggcg gcgcgcgttg gccaggccct ggcgcaggcg gtcgagcagg aaacggcggt
     1441 tggggagctg ggtcagcgtg tcgacgaagg cgagattgcg gatcgcttcc tcggcggcct
     1501 tgcgttcggt gatgtcgatg atcaccccgt cgagccggag cggttcatca tcggcgtcga
     1561 ccgccacgcg gccgcgctcg ctgacccaat ggacgcgccg gtcgcgcccg cgcatgcggt
     1621 attccacctc gtagttggtg tgactgatgc gtgcgctctc gatcgcggcg acgatcgtgg
     1681 ggacgtcctc atgcaggatg aagtctccga acgacaatcg accggacatg aaatcctcgg
     1741 gtgtcgcccc ggtgagcgcg gtgatcccgc ggctgatgtg catcaccctc cagggcggtt
     1801 cgatctggca gcggaagacg acgccgggca cgttctcgac cagggtgcgg aactcgtcgc
     1861 ggctgtgcgc caggtgcgac gcggcggcac gcatgcggcg gcggtggcgg gcgttgctgt
     1921 gcatcaccgc gaacagcatc aggctgagag cgctgccacc gtagaggatc agggcgggtt
     1981 ccgcgctcgc gtggtcggcc tcga
//
LOCUS       DAOQNN010000543         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_48384_length_1998_cov_8.074627, whole genome shotgun sequence.
ACCESSION   DAOQNN010000543 DAOQNN010000000
VERSION     DAOQNN010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48384_length_1998_cov_8.074627"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..313)
                     /locus_tag="PKV97_17120"
     CDS             complement(14..313)
                     /locus_tag="PKV97_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07659.1"
                     /translation="MMPSDLDQLRADFKTVWDYRRNAGHLSATEHREQYARARNEANS
                     RKDDPDWVRRAAAEYRAEAAQIEKERARANQIAEEVRAARASKTSQRKSIVARKS"
     gene            complement(300..1682)
                     /locus_tag="PKV97_17125"
     CDS             complement(300..1682)
                     /locus_tag="PKV97_17125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNB07660.1"
                     /translation="MIPLFSIEAETALLGALMIDSSCRKYIEVDLRAAHFYREDHQRI
                     YETILWLSDEKKREADPIAVYDAMQAKGFSGEACAWALEVGGSAYSTAGVKRHAEIIV
                     EKANLRALQAAAGEIIDALSGPGTAAEKIDAAQRSVMAVSDTAVLGERKPQRLSGLIP
                     GYLDAVGARWEKQGGGLDTGFPSVDTRVNGGLDEGWFVVLAGRPGMGKTSLALQIALH
                     FAHQGRPALFCSQEMQSGQLIDRAVSIEGRVPLGKVISGHGMTDDDHDRFQFGLSRIA
                     DAPLYIDEQGALRIEDVRRKAREVKRETGGRLGLLVIDYLQLMTADGENRTREMTAIT
                     GAIKSLAKELRVPIIALSQLNRGVENRPNKRPVMADLRESGSIEQDADVIWALYRDEV
                     YNPDSMDKGLAELLWLKNRYGEGSGMTPLVWHGPFVRFDSMFGDMPSAAAERDGTASP
                     EKSGRFNDAF"
     gene            complement(1679..>1998)
                     /locus_tag="PKV97_17130"
     CDS             complement(1679..>1998)
                     /locus_tag="PKV97_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07661.1"
                     /translation="SQRRFEDEGLLSWGVWSPDEQAFVDVFNANIGDLCPQVADWTEK
                     RSELTKVAVAGKMNLDQWGEFWRFVRDGCEFRFPISYEWFLVRENFKAVAGGQYGKEG
                     KAA"
BASE COUNT          359 a          688 c          555 g          396 t
ORIGIN      
        1 gaattttaca acctcagctt tttcgcgcaa ctatagattt acgctggctg gtttttgagg
       61 cgcgggcggc gcgcacttcc tcggcgatct ggttggcgcg ggcgcgctcc ttctcgatct
      121 gggcggcctc ggcgcggtat tcagcagcgg cgcgcctgac ccagtcaggg tcatccttgc
      181 gtgagttcgc ctcattgcga gcacgggcgt attgctcgcg gtgctccgtg gctgacagat
      241 gccccgcgtt gcgccggtag tcccagaccg tcttgaagtc ggcccgaagc tgatcgaggt
      301 cagaaggcat cattgaaccg cccagacttt tccgggctcg ccgtcccatc gcgctcggcc
      361 gccgcgctcg gcatgtcgcc gaacatggaa tcgaagcgca cgaatggacc atgccagaca
      421 aggggcgtca tgccgcttcc ctccccgtac cggttcttca gccacagcaa ctcggccagc
      481 cccttgtcca tcgagtccgg gttgtagacc tcgtccctgt aaagcgccca gatcacatcg
      541 gcgtcctgtt caatcgaacc cgactcacga agatcggcca taaccggccg cttgttgggt
      601 cggttctcga cgccccggtt aagttgagac agcgcgatga tcgggacgcg caactccttc
      661 gccagcgact ttatcgcccc cgtgatggcg gtcatctcgc gcgtccggtt ctctccgtct
      721 gcggtcatga gctggagata gtcaatcacc agcaggccga gccttccgcc cgtctcgcgc
      781 ttcacctcgc gggccttgcg ccgaacatcc tcgatccgaa gcgcgccctg ctcgtcgatg
      841 tacagcggcg catcggcgat gcgggacaga ccaaactgga atcggtcatg gtcgtcatcg
      901 gtcatcccat gacctgaaat cacctttccg agtggaacac gaccctcaat tgacacagcc
      961 cggtcaatga gctgtccgct ctgcatttcc tggctgcaaa acagcgccgg acggccttgg
     1021 tgggcaaagt ggagcgcgat ctgaagcgcg agagatgttt ttcccatgcc tgggcgcccg
     1081 gcgagaacga cgaaccagcc ttcatcaagg ccaccattga cccgggtgtc gacggatgga
     1141 aagcctgtat ccaacccgcc gccttgcttc tcccacctgg cgccgacagc atcaaggtat
     1201 ccagggatca gcccactcag gcgctgcggc ttacgctctc caagcaccgc ggtgtccgag
     1261 acggccatca ccgacctctg tgccgcgtcg atcttctcgg ccgccgtccc cgggccggac
     1321 agcgcatcaa tgatttcccc cgccgcagcc tgcagcgccc gcaggttcgc cttctcgacg
     1381 atgatttcag cgtgccgctt gacgcctgcc gtggagtagg ccgaaccgcc aacctccagc
     1441 gcccaagcgc aggcctcgcc cgaaaacccc ttagcctgca tggcatcgta gacagcgatg
     1501 gggtcggcct cgcgcttctt ctcatccgac agccagagga tcgtttcgta gatgcgctga
     1561 tggtcttctc ggtagaagtg cgccgcgcgc agatccacct cgatgtactt ccggcacgac
     1621 gagtcaatca tcaacgcgcc aagcagcgcg gtttctgcct caatggaaaa caatgggatc
     1681 atgctgcctt accctctttg ccgtactgac cgccggccac ggccttgaag ttctctctca
     1741 cgaggaacca ttcataggag atcgggaacc tgaactcgca tccgtctctg acgaatcgcc
     1801 agaactcgcc ccactggtca aggttcatct tcccagccac ggcaaccttc gtgagttcgc
     1861 tccgcttctc cgtccagtcc gccacctgcg ggcacaggtc gccaatgttg gcgttgaaca
     1921 cgtccacgaa cgcctgctcg tcgggcgacc acacgcccca ggacagcagc ccctcgtcct
     1981 cgaaccggcg ttgggata
//
LOCUS       DAOQNN010000544         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_48468_length_1996_cov_7.062339, whole genome shotgun sequence.
ACCESSION   DAOQNN010000544 DAOQNN010000000
VERSION     DAOQNN010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48468_length_1996_cov_7.062339"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1273
                     /locus_tag="PKV97_17135"
     CDS             134..1273
                     /locus_tag="PKV97_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNB07662.1"
                     /translation="MRDILIVGAGKIGTVIADLLAGSGDYAVTVADRDPAAVERVGAE
                     LARVQACALDVADADALAAELEGRWAVIDAGPFDIGMRIAAAAVAQRVHYLNLTEDVA
                     STRRVRELARGAHSALIPQCGLAPGFISIVAHDLAARFEELRDVRMRVGALPKYPSNG
                     LKYNLTWSTDGLINEYLNPCEAIVDGVRREMPALEELEHFSLDGDDYEAFNTSGGLGT
                     LCDTLEGRVRNLNYRTVRYRGHRDVMKLLLHDLRLGERRALLKDILESAIPVTMQDVV
                     LVFVTVSGRREGLLMQETFARKLYAAEVNGRLRSAIQLTTASALCAVLDLLAAGRLPQ
                     AGFVRQEDVDFCDFVTNRFGRHFLTDSEEVRCAASGVLAGPASGM"
     gene            1293..>1996
                     /locus_tag="PKV97_17140"
     CDS             1293..>1996
                     /locus_tag="PKV97_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB07663.1"
                     /translation="MLSNARFLPLGLDAPRLREGALAVHSPIDGSLLARLAPQDGATT
                     DAAIARSVAAFEAWGRVPAPRRGELVRRFAQALREHKALLAELVTLECGKIRSEGEGE
                     VQEMIDICDFAVGLSRQLHGLTIASERPGHALRESWHPLGPVAIVTAFNFPVAVWAWN
                     AAIALVCGDTLLWKPSERTPLCALACQRLLEQAAAGMEEVPRGLSAVVVGGAERAVQL
                     ADDRRVALLSATGSCA"
BASE COUNT          266 a          738 c          716 g          276 t
ORIGIN      
        1 gtcgtggcgg cgctggcggc gccctggctg ggctgacgag gcgccggcgc cccgcggccc
       61 gcgccgcgca accatttccc cgtgcgcagg gtctcaaggc ctgaaaccag ccaatccgcg
      121 cggggagggc gtcatgaggg acatcctgat cgtcggcgcg ggcaagatcg gcacggtgat
      181 cgccgatctg ctcgccggga gcggagacta tgcggtgacg gtcgcggatc gcgaccccgc
      241 cgcggtcgag cgcgtggggg cagagctcgc gcgcgtgcag gcctgcgcgc tggacgtcgc
      301 cgacgccgat gcgctcgccg cggagctgga agggcgctgg gcggtgatcg acgccgggcc
      361 cttcgacatc ggcatgcgca tcgccgcggc cgcggtggcg cagcgggtgc attacctcaa
      421 cctcaccgag gacgtcgcca gcacgcgccg ggtacgcgag ctcgcccgcg gcgcgcacag
      481 cgcgctgatc ccgcaatgcg ggctggcgcc cggcttcatc tccatcgtcg cccacgacct
      541 cgccgcgcgc ttcgaagaac tgcgcgacgt gcgcatgcgc gtcggcgcac tgcccaagta
      601 cccctccaac ggcctcaagt acaacctgac ctggagcacc gacggcctca tcaacgagta
      661 cctcaacccc tgcgaggcga tcgtcgacgg ggtgcgccgc gagatgccgg cgctcgagga
      721 gctcgagcac ttctcgctcg acggcgacga ctacgaggcc ttcaacacct cgggcgggct
      781 gggtacgctg tgcgacacgc tcgagggccg ggtgcgcaac ctcaactacc gcaccgtgcg
      841 ctaccgcggc caccgcgacg tgatgaagct gctgctgcac gacctgcgcc tgggcgagcg
      901 ccgcgcgttg ctcaaggaca tcctggagtc ggcgatcccg gtgaccatgc aggacgtggt
      961 gctggtcttc gtcaccgtca gcggccggcg cgagggcctg ctgatgcagg agaccttcgc
     1021 gcgcaagctc tacgccgccg aggtcaacgg ccgcctgcgc agcgcgatcc agctcaccac
     1081 cgcgagcgcg ctgtgcgcgg tgctcgacct gctcgccgcc ggccgcctgc cgcaggcggg
     1141 cttcgtgcgc caggaggacg tggatttctg cgacttcgtc accaaccgct tcggccgcca
     1201 cttcctgacc gacagcgagg aggtgcgctg cgccgccagc ggcgtcctcg ccgggccggc
     1261 ctccggcatg tgactccgag gaggacccca gcatgttgag caacgcccgc ttccttcccc
     1321 tcggcctcga cgccccccgc ctgcgcgagg gcgcgctggc ggtgcatagc ccgatcgacg
     1381 gcagcctgct cgcgcgcctc gcgccgcagg atggcgccac caccgatgcc gcgatcgcgc
     1441 gcagcgtggc ggcctttgag gcctggggcc gcgtgccggc gccgcgccgt ggcgagctgg
     1501 tgcgccgctt cgcgcaggcg ctgcgcgagc acaaggccct gcttgccgaa ctggtcaccc
     1561 tcgagtgcgg caagatccgc agcgagggcg aaggcgaggt gcaggagatg atcgacatct
     1621 gcgacttcgc ggtcggcctg tcgcgccagc tgcacggcct gaccatcgcc tccgagcgcc
     1681 ccggccatgc cttgcgcgag agctggcacc cgctcggccc ggtggcgatc gtcaccgcct
     1741 tcaacttccc ggtcgcggtg tgggcgtgga acgcggcgat cgcgctggtg tgcggcgaca
     1801 ccctgctgtg gaagccctcc gagcgcaccc cgctgtgcgc gctcgcctgc cagcgcctgc
     1861 tcgaacaggc ggcggcgggc atggaggagg tgccgcgcgg gctctcggcg gtggtcgtgg
     1921 gcggcgccga gcgcgccgtg cagctcgccg acgaccgccg cgtggcgctg ctctcggcca
     1981 ccggcagctg cgcgat
//
LOCUS       DAOQNN010000545         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_48645_length_1992_cov_5.669076, whole genome shotgun sequence.
ACCESSION   DAOQNN010000545 DAOQNN010000000
VERSION     DAOQNN010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48645_length_1992_cov_5.669076"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKV97_17145"
     CDS             complement(<1..425)
                     /locus_tag="PKV97_17145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB07664.1"
                     /translation="MKALKVLQLLPALDSGGVERGTLEIARALVAAGHESVVLSRGGR
                     LVGQLQDEGSRHLARDLGRKSPTTFLHYRALRRLFEAERFDIVHARSRLPAWVAWLAW
                     RGMPADARPHFVTTVHGMHSVSRYSAIMCAGERVIAVSDT"
     gene            complement(433..753)
                     /locus_tag="PKV97_17150"
     CDS             complement(433..753)
                     /locus_tag="PKV97_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HNB07665.1"
                     /translation="MSSKKPAKTKRASLPRAADFSKAFHKDWERFSRSGRFDLKRLKE
                     VMLLLIANDAPLGPEWFDHPLKGEWADHRECHVGGDFLLIYSADDRQITFVRAGTHAD
                     LFEG"
     gene            complement(731..1003)
                     /locus_tag="PKV97_17155"
     CDS             complement(731..1003)
                     /locus_tag="PKV97_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HNB07666.1"
                     /translation="MAQSSMLHIRVDDETKTQATEALAAMGLSMSDAVRIFLKRVVND
                     QAFPLELKVPNAETRAAMEEARAMMKAHQARFESTDALFDELEKAR"
     gene            complement(1100..1693)
                     /locus_tag="PKV97_17160"
     CDS             complement(1100..1693)
                     /locus_tag="PKV97_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNB07667.1"
                     /translation="MGLALRKEKHYTYADYLQWPDETRYELIDGEAFLMSPAPLVEHQ
                     EVAGEVYYQLRNQLDGQPCRPYIAPVDVRLPRKDEADAAIDTVVQPDILVVCDPRKID
                     RRGVRGAPDWVLEVLSPSTAAHDQIAKRRTYERAGVREYWLVHPGDRTLTVYLLDNGQ
                     YGRPEIYELKDATPIGVLPGVSIAWDALVARLPKPEY"
BASE COUNT          291 a          616 c          730 g          355 t
ORIGIN      
        1 gtgtcgctga ctgcgatcac gcgctcgccc gcgcacatga tggcgctgta gcggctgacc
       61 gagtgcatgc cgtgcaccgt ggtgacgaag tgtgggcggg cgtcagccgg catgccgcgc
      121 caggccagcc aagccaccca ggcgggcagg cgcgagcggg catggacgat gtcgaagcgc
      181 tcggcctcga agagcctgcg cagcgcgcgg tagtgcagga aggtggtcgg cgacttgcgc
      241 ccgaggtcgc gcgcgaggtg gcgcgagcct tcgtcctgca actgcccgac caggcgcccg
      301 cccctggaca gcaccaccga ttcgtgcccg gccgcgacca gcgcgcgcgc gatctcgagc
      361 gtgccacgct cgacgccgcc gctgtccagc gcggggagaa gttgcaggac cttgagggct
      421 ttcattcgtg cctcatccct cgaagagatc cgcatgggtg cccgcccgga cgaaggtaat
      481 ctgtcggtca tcggcgctgt agatgagcag gaagtcgccg ccgacgtggc attcgcggtg
      541 atcggcccac tcgcccttga gcgggtggtc gaaccactcc gggccgagcg gggcgtcgtt
      601 ggcgatgagc agcagcatga cttccttgag ccgcttgagg tcgaaacggc cggagcggct
      661 gaatcgttcc cagtccttgt ggaaggcctt gctgaaatcc gcggcgcgcg gcagcgatgc
      721 tcgcttggtt ttagcgggct ttttcgagct catcgaagag ggcgtcggtc gattcgaaac
      781 gtgcctggtg tgccttcatc atggcgcgcg cctcttccat ggcggcgcgc gtttcggcgt
      841 tggggacctt gagttcgagc gggaaggcct ggtcgttgac gacgcgcttg aggaagatgc
      901 gcacggcgtc ggacatcgac aggcccatgg cggccagcgc ctcggtggcc tgggtcttcg
      961 tttcgtcgtc gacgcggatg tggagcattg aactctgtgc catggcttcc tcgcttgcgg
     1021 agtgcgaatt atgtatcgca tatgagatgc gatcaagcca ggcggttcct gtggcgggag
     1081 cgaagccggg tcggccagat caatactccg gcttgggcag gcgtgcgacc agcgcgtccc
     1141 aggcgatcga cacgcccggc agcacgccga tcggggtcgc gtccttcagc tcgtagatct
     1201 ccggccggcc gtactggccg ttgtcgagca gatagacagt gagggtgcgg tcccccgggt
     1261 gcaccagcca gtattcgcgc acgccggcgc gctcgtaggt gcggcgcttg gcgatctggt
     1321 cgtgggcggc ggtgctgggg gagagcacct cgagcaccca gtcgggcgcg ccgcgcacgc
     1381 cgcggcggtc gatcttgcgc gggtcgcata ccacgaggat atcgggctgc accacggtgt
     1441 cgatggcagc gtcggcttcg tccttgcgcg gcaggcggac gtcgaccggg gcgatgtagg
     1501 ggcggcaggg ctggccgtcg agctggttgc gaagctgata gtagacctcg ccggcgacct
     1561 cctgatgctc gaccagcggt gcaggtgaca tcaggaaggc ttcgccgtcg atcagctcgt
     1621 agcgtgtctc gtccggccac tgcaggtagt cggcgtaggt gtagtgcttt tccttgcgca
     1681 gcgcgaggcc catggcggtg ctccgatccg gttcggggga tagcggtatt gtaggccggc
     1741 ggcgggcccg ccttgtatac gcgatgtttc gtgtaggagc ggcgccagcc gcgaatacgg
     1801 ccgtcgggtg cgcgacgtgg gcggacgaag ctcaaccgcc gctttcgcgc cttgcggcgc
     1861 ttatatggac gcctcctaca gaaaaccgtc gcggtgcggg gggctcgaac ttgtgctcac
     1921 gctccgaacc gtgccagcac cgcctcggcc acgcgctcgg cttcggccag gcgctcgggg
     1981 ggcgtgctca gg
//
LOCUS       DAOQNN010000546         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_48683_length_1991_cov_18.239153, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000546 DAOQNN010000000
VERSION     DAOQNN010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48683_length_1991_cov_18.239153"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PKV97_17165"
     CDS             complement(<1..764)
                     /locus_tag="PKV97_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07668.1"
                     /translation="MGVPIASGLPDYGPAGTINFNPELYSAKLVEKFYKTTVFGEIAS
                     TDYEGEITGFGAQVKIRTIPDVTVSDYVVGAGLNPQYPTNNSVTLAIDQAKSFAVALS
                     TVDSRQSDLDLADIFANDGSIQLRIAADADMLTTIPADVAAANSGNTAGADSANINLG
                     SSTTPRAVSKTDVVDFIVDCGTVLDEQNVSDEGRWMVAPPWFIALIKKSDLRIASLAG
                     DGVSILRNGKVGEIDRFTIYQSRNLLTQTSPGPASYV"
     gene            complement(799..1647)
                     /locus_tag="PKV97_17170"
     CDS             complement(799..1647)
                     /locus_tag="PKV97_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07669.1"
                     /translation="MAAVPDANTPKAPNPPEDPRHSDPTYWKHRFEAVAGRLRAREDE
                     HKAAIGTLRQENDQLRTEILRLQQATPTSTPQATINLAEFFTPEQIKNLGEDDATAMA
                     QAAVNAAMKVARETVTAEIKPLLTRQQEDSEAEAARKVREFEEAVEEAVPNYRVLDKD
                     EGWLTWLDEEDAATGLVRQEILTRHCARLDAPKVVKMFQAYLAQAAAAAPQVPEPPIA
                     PSGSGATGGDTPPQLPRGAGTGYPTQAEIKDFFKRSATKKPGDVGFVTDKERAEFEAR
                     LRLPRR"
BASE COUNT          273 a          621 c          697 g          400 t
ORIGIN      
        1 acgtagctcg cggggcccgg gctcgtctgc gtcagcaggt tgcggctctg gtagatcgtg
       61 aagcggtcga tctcgcccac cttgccgttg cgcaggatgg acacgccatc accggccagc
      121 gacgcgatgc gcagatcgga cttcttgatc agggcgatga accacggcgg tgccaccatc
      181 caacggccct cgtccgacac gttctgctcg tcgagcaccg tgccgcagtc gacgatgaaa
      241 tccaccacgt cggtcttgga gaccgcgcgc ggcgtcgtcg aactgccgag gttgatgttg
      301 gccgaatcgg cgccggcggt gttgcccgag ttggccgccg ccacgtccgc agggatcgtg
      361 gtgagcatgt ccgcgtcggc cgcaatgcgc agctggatcg aaccgtcgtt ggcgaagatg
      421 tcggccaggt cgaggtccga ctggcgcgaa tccaccgtcg acagcgcgac cgcgaacgac
      481 ttggcctggt cgatggccag cgtcaccgag ttgttggtcg ggtactgcgg gttgaggccc
      541 gcgcccacca cgtagtcgga gacggtcacg tccgggatcg tgcggatctt gacctgcgcg
      601 ccgaagcccg tgatctcgcc ctcgtagtcg gtcgaggcga tctcgccgaa cacggtggtc
      661 ttgtagaact tctcgaccag cttggccgag tacagttccg ggttgaagtt gatggtgcct
      721 gccgggccgt agtccggaag acccgatgcg atgggaacgc ccatggtgtg ctcctgtgcg
      781 aaaggcgttg ccggtcacct accggcgcgg gagcctcaac ctcgcttcga actctgcgcg
      841 ttccttgtcc gtcacgaatc cgacatcacc gggcttcttg gttgctgagc gcttgaagaa
      901 gtccttgatc tcggcctgag tcgggtagcc cgttccggcg ccacgcggca gttggggagg
      961 ggtgtcacca ccagtcgctc cgctgccgct tggtgcaatc gggggttccg gcacctgcgg
     1021 ggcggccgca gccgcttgcg cgaggtatgc ctggaacatc ttcaccacct tcggcgcgtc
     1081 gagacgcgcg caatggcgag tgaggatctc ctgccgaacc agtccagtcg ctgcgtcttc
     1141 ctcgtccagc caggtcagcc agccctcgtc tttgtcgagg actcggtagt tcgggacggc
     1201 ctcttcaacg gcctcttcga actctcgcac cttgcgcgcc gcttcggctt ccgagtcctc
     1261 ctgctgccgg gtgagcagcg gcttgatctc ggcggtcacc gtctcgcgag cgaccttcat
     1321 cgctgcgttc acggcggcct gggccatcgc ggttgcgtcg tcttcgccga ggttcttgat
     1381 ctgctcgggt gtgaagaact ccgcgaggtt gatcgtggcc tggggcgtcg aggtgggcgt
     1441 cgcctgctgc agacggagga tttccgttcg caactgatcg ttttcctgac ggagcgtgcc
     1501 gatcgctgcc ttgtgctcgt cctctcgggc gcggagtcga ccggcgacgg cttcgaagcg
     1561 atgcttccag tacgtcgggt cgctgtgccg cggatcctcg ggcgggttgg gcgccttggg
     1621 tgtgttggcg tcagggacgg cggccacagg tgcgttcggt tcatccgagg gcgccactgg
     1681 tgccgtgggc tccgggtcgg ccagattcgg ggtgcgcggg gtggtagccg ccgcaacacg
     1741 ccgcacaacg gcgcgaggga gtcgcgtttc attcgaaggc tgtgaagcct gctgcatgtg
     1801 ggttctccga ggtccggggc gcgatcagcg tccggggaat cggttgcgta gggggccgga
     1861 ggcgagccct actgttggtg cggtacgggg cgagcctgtg aggtgacagg ggccccgttc
     1921 cgggtcagct tctgctcggc ctgtgcgatg tcggcgatca actcgtcgac tgcgagagcc
     1981 cgtccttgcc a
//
LOCUS       DAOQNN010000547         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_48737_length_1990_cov_11.249096, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000547 DAOQNN010000000
VERSION     DAOQNN010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48737_length_1990_cov_11.249096"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PKV97_17175"
     CDS             complement(<1..895)
                     /locus_tag="PKV97_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB07670.1"
                     /translation="MASNKASFNWEDPLLLDLQLTETERMVRDTARAYSQDKLLPRVQ
                     EAFRHEKTDAAIFREMGELGLLGATIPEQYGGSGLNYVCYGLIAREVERVDSGYRSMM
                     SVQSSLVMVPINEFGTEAQKQKYLPKLATGEWIGCFGLTEPNHGSDPGSMITRARKVD
                     GGFSLSGSKMWITNSPIADVFVVWAKDDEGQIRGFILEKGWKGLSAPAIHGKVGLRAS
                     ITGEIVMDEVFVPEENAFPTVRGLKGPFTCLNSARFGIAWGALGAAEACYETARQYTM
                     DRNQFGRPLAANQLIQKKLADM"
     gene            1038..1934
                     /locus_tag="PKV97_17180"
     CDS             1038..1934
                     /locus_tag="PKV97_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB07671.1"
                     /translation="MRKKIPSTAALLAFEAAARHESFTRAAEELALTQSAICRQIGAL
                     EDFLGVPLFRRTRRGVQLTEAGLSYSRQIAPRLDAIEHDTLAVMAHHGAGSVLELAVV
                     PTFATRWLLPRLAGFQARNPDVVVNMHTQTRPFLFDQTEFDAAIYFGDTGWPGTEAHF
                     LMREYPVPVCSPALPGVREQMSPDEVAELPLLQQTTRPYAWRQWFASAGVRPANDMGG
                     MRLELFSMLAQAAIERMGVALIPPFLIQQELAAGSLISPCLRSFPSTRAYYLIVPEGK
                     AERPALTNFREWLVEEARATPL"
BASE COUNT          302 a          683 c          676 g          329 t
ORIGIN      
        1 gcatgtcggc gagcttcttc tggatgagct ggttggcggc gagcggacgg ccgaactggt
       61 tacggtccat cgtgtactgg cgggcggtct cgtagcaggc ctcggcggcg ccgagcgcac
      121 cccaggcgat gccgaagcgc gccgagttga ggcaggtgaa ggggcccttg agcccgcgca
      181 cggtcgggaa ggcgttctct tccggcacga agacctcgtc catgacgatc tcgccggtga
      241 tcgaggcacg caggccgacc ttgccgtgga tggcgggcgc ggagaggccc ttccagccct
      301 tctcgaggat gaagccgcgg atctggcctt cgtcgtcctt ggcccacacc acgaacacgt
      361 cggcgatcgg gctgttggtg atccacatct tgctgccgga gaggctgaag ccgccatcga
      421 ccttgcgcgc acgggtgatc atggagcccg ggtcggagcc gtggttcggc tcggtgaggc
      481 cgaagcagcc gatccattcg ccggtggcga gcttgggcag gtacttctgc ttctgcgcct
      541 cggtaccgaa ttcgttgatc ggcaccatca ccagcgagct ctgcacgctc atcatcgaac
      601 ggtagccgga gtcgacacgc tcgacctcgc gggcgatcag gccgtagcac acgtagttga
      661 ggccggagcc gccgtactgc tcggggatgg tggcgccgag cagaccgagt tcgcccatct
      721 cgcggaagat cgcggcatcg gtcttctcgt ggcggaaggc ctcctgcacg cgcggcagca
      781 gcttgtcctg gctgtaggcg cgcgcagtgt cgcgcaccat gcgctcggtc tcggtgagct
      841 gcaggtcgag cagcagcggg tcttcccagt tgaagctagc cttgttcgaa gccatgtcgt
      901 gtctcctcgt gcgatcggtc gtgcaggcgg ctgcctgtgt gtgattcgca tcctatgaac
      961 tccggaagcg cagctcaagc gatatttctg cagtaatctg tgcgcttccg gaactccgag
     1021 cgataacgcc gacccccatg cgcaagaaga tccccagcac cgccgcgctg ctcgccttcg
     1081 aggctgccgc gcgccacgag agcttcaccc gcgccgccga ggagctcgcg ctcacgcaga
     1141 gcgcgatctg ccggcagatc ggcgcgctcg aggacttcct cggcgtgccg ctgttccgcc
     1201 gcacccggcg cggcgtgcag ctcaccgagg caggactgag ctacagccgc cagatcgccc
     1261 cgcggctgga tgcgatcgag cacgacacgc tcgcggtcat ggcacaccac ggcgccggca
     1321 gcgtgctcga gctggccgtc gtgccgacct tcgccacgcg ctggctgctg ccccgcctgg
     1381 ccggcttcca ggcgcggaac ccggacgtgg tggtgaacat gcacacccag acgcggcctt
     1441 tcctcttcga ccagaccgag ttcgacgccg cgatctactt cggcgacacc ggctggccgg
     1501 gcaccgaggc gcacttcctg atgcgggaat atccggtgcc ggtatgcagc ccggccctgc
     1561 ccggcgtgcg cgagcagatg agcccggacg aggtcgccga gctgccgctg ctgcagcaga
     1621 ccacccgccc ctacgcctgg cggcagtggt tcgcctcggc cggcgtgcgc cccgcgaacg
     1681 acatgggcgg catgcgcctg gagctgttct cgatgctcgc gcaggcggcg atcgagcgca
     1741 tgggcgtggc gctgatcccg cccttcctga tccagcagga gctcgccgcc ggcagcctga
     1801 tcagcccctg cctgcgcagc ttcccgagca cgcgggcgta ctacctgatc gtgcccgagg
     1861 gcaaggcgga acggccggcg ctgacgaact tccgcgaatg gctggtcgag gaagcgcgcg
     1921 ccacgccgct gtaggggcgc cctcaggcgt tcacataggc acggatgccc gctgcgatga
     1981 actccaccgc
//
LOCUS       DAOQNN010000548         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_49050_length_1983_cov_9.487033, whole genome shotgun sequence.
ACCESSION   DAOQNN010000548 DAOQNN010000000
VERSION     DAOQNN010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49050_length_1983_cov_9.487033"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /gene="gltD"
                     /locus_tag="PKV97_17185"
     CDS             complement(<1..229)
                     /gene="gltD"
                     /locus_tag="PKV97_17185"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357058.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HNB07672.1"
                     /translation="MGKPTGFMEYQRLSEAYEPVDKRLKTYKEFVVRLTDEQASVQGA
                     RCMDCGIPFCNNGCPVNNIIPDWNDLVYRGHW"
     gene            complement(230..>1983)
                     /locus_tag="PKV97_17190"
     CDS             complement(230..>1983)
                     /locus_tag="PKV97_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNB07673.1"
                     /translation="DQIQIKMAQGAKPGEGGQLPGHKVSEYIGFLRHSVPGVGLISPP
                     PHHDIYSIEDLAQLIHDLKNTNPAASISVKLVSEIGIGTVAAGVAKAKADHIVVAGHD
                     GGTGASPWSSIKHAGSPWELGLAETQQTLVLNRLRGRVRLQVDGQIKTGRDVVIGALL
                     GADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRARFSGQPEYVVNYFFFVA
                     EEVRELMAQLGVRTFDELIGRADLLDMKKGIAHWKAQGLDYSRIFYLPNVPAEVPRLH
                     RETQDHGLDKALDKQLVVLAKPALEKGEKVNIDLPVRNINRTVGAMLSGEVAKRYGHA
                     GLPNDTIHIRLNGTAGQSFGAFLARGVTLELVGEGNDYVGKGLSGGRIIVRPKAEFRG
                     DTPQNIIVGNTVLYGATEGEVYFAGVAGERFAVRNSGATAVVEGVGDHGCEYMTGGTV
                     VVLGETGRNFAAGMSGGVAYVLDEDGTFEQRCNMAQVALEPLPEEIAARKGSESGDEL
                     ESHGRVDIDHLTMGDELILKGLIERHVRFASSVRAREILNNWGLWRKKFVKVFPHEYR
                     RALTEMAQQREAEKEAA"
BASE COUNT          280 a          664 c          694 g          345 t
ORIGIN      
        1 gccagtggcc gcgatacacg aggtcgttcc agtccgggat gatgttgttg accgggcagc
       61 cgttgttgca gaacgggatg ccgcagtcca tgcagcgcgc gccctggacc gacgcctgct
      121 cgtcggtgag gcggacgacg aactccttgt aggtcttcag gcgcttgtcg accggctcgt
      181 aggcctcgga cagacgctga tattccatga atccggtggg cttgcccatt tatgcggcct
      241 ccttctcagc ttcgcgctgc tgagccatct cggtcagcgc gcggcggtac tcgtgcggga
      301 acaccttgac gaacttcttc cgccagaggc cccagttgtt caggatctcg cgcgcacgca
      361 cgctgctggc gaagcggacg tggcgctcga tcaggccctt gaggatgagc tcgtcgccca
      421 tcgtcaggtg gtcgatatcg acgcggccgt gggactcgag ctcgtcgccc gactccgagc
      481 ccttgcgcgc ggcgatctcc tcgggcagcg gctcgagcgc gacctgcgcc atgttgcagc
      541 gctgctcgaa cgtgccatcc tcgtcgagca cgtaggccac gccgcccgac atgccggcgg
      601 cgaagttgcg cccggtctcg ccgagcacga ccacggtgcc gccggtcatg tactcgcagc
      661 cgtggtcgcc cacgccctcc accaccgcgg tggcgccgga gttgcgcacc gcgaagcgct
      721 cgcccgcgac gccggcgaag tagacctcgc cctcggtcgc gccgtagagc acggtgttgc
      781 cgacgatgat gttctgcggc gtgtcgccgc ggaactcggc cttcgggcgc acgatgatgc
      841 ggccgcccga aaggcccttg ccgacgtagt cgttgccctc gcccaccagc tcgagcgtga
      901 cgccgcgcgc caggaaggcg ccgaagctct gcccggcggt gccgttgagg cggatgtgga
      961 tggtgtcgtt gggcaggccg gcatggccgt agcgcttggc cacctcgccc gagagcatcg
     1021 cgcccacggt gcggttgatg ttgcgcaccg gcaggtcgat gttgaccttc tcacccttct
     1081 ccagtgccgg cttggcgagc acgacgagct gcttgtcgag tgccttgtcg aggccgtggt
     1141 cctgcgtctc gcgatgcagg cgcggcacct cggcgggcac gttgggcagg tagaagatgc
     1201 gcgagtagtc gaggccctgc gccttccagt gcgcgatgcc cttcttcatg tcgagcaggt
     1261 cggcacggcc gatgagctcg tcgaaggtcc gcacgccgag ctgcgccatc agctcgcgca
     1321 cttcctcggc gacgaagaag aagtagttga ccacgtactc gggctggccc gagaagcgcg
     1381 cgcgcagcac cgggtcctgg gtggcgacgc cgaccgggca ggtgttgagg tggcacttgc
     1441 gcatcatgat gcagccctcg acgacgagcg gcgcggtggc gaagccgaac tcgtcggcgc
     1501 cgagcagcgc gccgatgacg acgtcgcggc cggtcttgat ctggccgtcg acctgcaggc
     1561 gcacgcgtcc gcgcaggcgg ttgagcacca gggtctgctg ggtctcggcc agcccgagct
     1621 cccacggcga gccggcgtgc ttgatcgagc tccacgggct cgcaccggtg ccgccgtcat
     1681 ggccggcaac cacgatgtgg tcggccttgg ccttggccac gcccgccgcc accgttccga
     1741 tgccgatctc ggacaccagc ttgaccgaga tgctcgcggc cgggttggtg ttcttgaggt
     1801 cgtggatgag ctgcgcgagg tcctcgatgg agtagatgtc gtggtgcggc ggcggcgaga
     1861 tgaggccaac gcccggcacc gagtgacgca ggaagccgat gtactccgag accttgtggc
     1921 cgggcagctg gccgccctcg ccgggcttgg cgccctgggc catcttgatc tggatctggt
     1981 cgg
//
LOCUS       DAOQNN010000549         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_49651_length_1971_cov_13.164405, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000549 DAOQNN010000000
VERSION     DAOQNN010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49651_length_1971_cov_13.164405"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PKV97_17195"
     CDS             complement(<1..1063)
                     /locus_tag="PKV97_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB07674.1"
                     /translation="MSNEQQVRIYNPSEEMVKNAAVSGMDGYRALCKEAEDDYEGYWG
                     RRAKELLDWKVPFTQVLDQSNAPFFKWFADGKLNVSYNCLDRNVANGLGDKVALIFEA
                     DGGEVTRITYKDLLSRVSKFANALRGMGVKKGDRVIIYLPMSIEGVVAMQACARIGAT
                     HSIVFGGFSAQALRDRIEDAAAVAVITSDGQFRGGKALPLKPIADEALSLGGCDTVKN
                     VIVVKRTGADIAMVAGRDSWFHDVVATQSEVCEPEWVEAEHPLFVLYTSGSTGKPKGV
                     QHSSGGYLLQAVLSMKYTFDIKPNDVYWCTADIGWVTGHTYITYGPLACGATEIVFEG
                     VPTYPDAGRFWKMIQDHKVS"
     gene            1352..>1971
                     /locus_tag="PKV97_17200"
     CDS             1352..>1971
                     /locus_tag="PKV97_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNB07675.1"
                     /translation="MTIIRQEDLIQSIADAFQYISYYHPLDYIQALGEAYAREESPAA
                     KDAIAQILTNSRMAAEGHRPICQDTGIAVVFLKVGMNVQWDAKMSVQEMIDEGVRRAY
                     NHPDNKLRASVLLDPAGKRQNSKDNTPAVVHYEVVAGDEVEVICAAKGGGSENKTKMV
                     MLNPSDSIVDWVLKTVPTMGAGWCPPGILGIGIGGTPEKAMLLAKES"
BASE COUNT          355 a          645 c          639 g          332 t
ORIGIN      
        1 tgctgacctt gtggtcctgg atcatcttcc agaagcggcc ggcgtccggg taggtgggca
       61 cgccctcgaa cacgatctcg gtcgcgccgc aggcgagcgg gccgtaggtg atgtaggtgt
      121 gacccgtgac ccaaccgatg tcggcggtgc accaatagac gtcgttcggc ttgatgtcga
      181 aggtgtactt catcgacagc acggcctgca gcaggtagcc gcccgaagag tgctgcacgc
      241 ctttcggctt gccggtggaa cccgaggtgt agagcacgaa cagcggatgc tcggcctcga
      301 cccactccgg ctcgcagacc tcggactggg tggccaccac gtcgtggaac cagctgtcac
      361 ggccggcgac catggcgatg tcggcaccgg tgcgcttgac gacgatgacg ttcttgacgg
      421 tatcgcagcc gccgagcgac agcgcctcgt cggcgatcgg cttgagcggc agcgccttgc
      481 cgccgcggaa ctggccgtcg gaggtgatca cggccacggc agcggcgtcc tcgatgcgat
      541 cgcgcagcgc ctgggcggag aagccgccga acacgatcga gtgggtggcg ccgatgcggg
      601 cgcaggcctg catcgcgacc acgccttcga tcgacatcgg caggtagatg atgacgcgat
      661 cgcccttctt gacgcccatg ccgcgcaggg cgttggcgaa cttgctgacg cgggagagca
      721 ggtccttgta ggtgatgcgg gtgacttcgc cgccgtcggc ttcgaagatc agcgcgacct
      781 tgtcgccgag gccgttggcc acgttgcggt cgaggcagtt gtaggagacg ttgagcttgc
      841 cgtctgcgaa ccacttgaag aagggcgcat tcgactggtc gaggacctgg gtgaagggca
      901 ctttccagtc cagcagctcc ttggcgcgac gcccccagta accctcgtag tcgtcctcgg
      961 cttccttgca cagcgcgcga taaccgtcca tgccggacac cgctgcgttc ttgaccatct
     1021 cttccgaggg attgtaaatg cggacttgtt gctcgttgga catgctcacc tctcctcaac
     1081 tgttcaaatg gttgaagcta cccgttgcgg gtgggctggt tgaacccagc ctatgatccg
     1141 cccgaacgcc gacgccccgt gagggccctc cccgtccgga cactcggatg ccgttcgaaa
     1201 cgccggcatt agagaacagc tatcttacac aggacttaca ccgcccggcc agcgacggcc
     1261 ggcggggccg gtggtagaat ccggcgccac tccgggcctt ccggtccggt cgcgtcattc
     1321 tgcccccatg tcattccgga gaccaagcgc catgacgatc attcgccagg aagacctcat
     1381 ccagtcgatc gcggatgcct tccagtacat cagctactac cacccgctcg actacatcca
     1441 ggcgctcggc gaagcctacg cgcgcgagga aagcccggcc gcgaaggacg cgatcgcgca
     1501 gatcctgacc aactcgcgca tggccgccga gggccaccgc ccgatctgcc aggacaccgg
     1561 catcgcggtc gtcttcctca aggtgggcat gaacgtgcag tgggatgcga agatgagcgt
     1621 ccaggagatg atcgacgagg gtgtgcgccg cgcctacaac cacccggaca acaagctgcg
     1681 cgcctccgtg ctgctcgacc ccgccggcaa gcgccagaac agcaaggaca acaccccggc
     1741 ggtggtgcac tacgaggtcg tcgcgggcga cgaggtcgag gtcatctgcg cggccaaggg
     1801 cggcggctcc gagaacaaga ccaagatggt catgctcaac ccctccgact cgatcgtcga
     1861 ctgggtgcta aagaccgtgc cgaccatggg cgccggctgg tgtccgccgg gcatcctcgg
     1921 catcggcatc ggcggcacgc ccgagaaggc catgctgctg gccaaggaat c
//
LOCUS       DAOQNN010000550         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_49739_length_1969_cov_7.956113, whole genome shotgun sequence.
ACCESSION   DAOQNN010000550 DAOQNN010000000
VERSION     DAOQNN010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49739_length_1969_cov_7.956113"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKV97_17205"
     CDS             complement(<1..208)
                     /locus_tag="PKV97_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB07676.1"
                     /translation="MNFAPIYLDWNATTPLDPAVREAMLPWLGAAEPARFGNASSRHE
                     YGRQARAAVDEARARVAAAVGAHAT"
     gene            complement(217..699)
                     /gene="iscR"
                     /locus_tag="PKV97_17210"
     CDS             complement(217..699)
                     /gene="iscR"
                     /locus_tag="PKV97_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HNB07677.1"
                     /translation="MRLTTKGRFAVTAMIDLAARQGDGPVTLAGIAERQSISLSYLEQ
                     LFGKLRRHKLVNSVRGPGGGYRLARAMDEITVADIIIAVDEPIDATQCGGKENCHDDH
                     VCMTHHLWSNLNQRIYAYLDSVSLEALVKHQVRPDADMTVLKSVRRRSRMPVHEIAAV
                     "
     gene            complement(906..1673)
                     /gene="cysE"
                     /locus_tag="PKV97_17215"
     CDS             complement(906..1673)
                     /gene="cysE"
                     /locus_tag="PKV97_17215"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNB07678.1"
                     /translation="MFSRLREDLASVRERDPAARSTLEVLTCYPGIHALMFHRLAHGA
                     WRRGWFWAGRFASHVGRLLTGIEIHPGAKIGRRVFIDHGMGVVIGETAEIGDDCTIYQ
                     GVTLGGTSLYRGTKRHPTLGRGVVVGAGAKVLGGFTVGDGAKVGSNAVVVKPVPAGAT
                     AVGNPARILDSDKDSERDAAREQKAEQFGFSAYGVTRDMDDPVAKALHGLLDHSVETD
                     RRLQAILARLEAAGIKLDDTVAPSDHFDPGRLSRMVD"
BASE COUNT          295 a          680 c          689 g          305 t
ORIGIN      
        1 ccgtagcatg cgccccgacc gccgcggcaa cccgcgcgcg cgcctcgtcc accgcggcac
       61 gcgcctggcg gccgtactcg tggcggctgg aggcgttgcc gaagcgcgcc ggctcggcgg
      121 cgccgagcca gggcagcatg gcctcgcgca ccgccggatc gagtggcgtg gtcgcgttcc
      181 agtcgaggta gatgggagcg aagttcatgc gcgacctcag accgcggcga tctcgtgcac
      241 cggcatgcgg ctgcgccgcc gcacgctctt gagtaccgtc atgtcggcgt cgggccgtac
      301 ctggtgcttg accagcgcct ccaggctcac cgagtcgagg taggcgtaga tgcgctggtt
      361 gaggttggac cacaggtggt gggtcatgca cacgtgatcg tcgtggcagt tttccttgcc
      421 gccgcactgg gtggcgtcga tcggctcgtc gaccgcgatg atgatgtcgg ccacggtgat
      481 ctcgtccatc gcccgcgcca gtcggtagcc gccgccggga ccgcgcacgc tgttaaccag
      541 cttgtgcctg cgcagcttgc cgaagagctg ctcgaggtag gacagcgaga tgctctggcg
      601 ctcggcgatg cccgccagcg tgacgggccc gtccccctgg cgggcagcca ggtcgatcat
      661 cgcggtcacc gcaaaccgtc ccttggtcgt aagtcgcatg aggctctcct cgaaggctgt
      721 ccgggctcgc gcgtgccggg ggccgccgaa tatcccatca tttttgtcga gtatatggat
      781 cccgacaaaa acggtcaact aatcgggagg caaggcttgc gatgcaaggc gcgaggtgcg
      841 ccgttgcagc cgcggcgcca gccgcgaaca cggcgctcga tcgtccggca ccgcaggcgt
      901 gcgaatcaat ccaccatccg cgacagccgg cccgggtcga aatggtcgct cggggcgacc
      961 gtgtcgtcga gcttgatccc cgcggcctcg agacgggcca ggatggcctg caggcggcgg
     1021 tcggtctcaa cggaatggtc gagcaggccg tgcagcgcct tggcgaccgg gtcgtccatg
     1081 tcgcgggtca cgccgtaggc gctgaagccg aactgctcgg ccttctgctc gcgcgcggcg
     1141 tcgcgctcgc tatccttgtc cgagtcgaga atgcgagccg gattgccgac ggcggtcgcg
     1201 ccggcgggca cgggcttcac caccaccgcg ttggagccga ccttggcgcc gtcacccacc
     1261 gtgaagccgc cgagcacctt ggcgcccgcc cccaccacca cgccacgccc cagggtggga
     1321 tggcgcttgg tgccgcggta gagggaggtc ccgccgaggg tgacgccctg gtagatcgtg
     1381 cagtcgtcgc cgatctccgc ggtttcgccg atcacgacgc ccatgccgtg gtcgatgaac
     1441 acgcggcggc cgatcttcgc tcccgggtgg atctcgatcc cggtgagcag ccggccgacg
     1501 tgactcgcga agcgccccgc ccagaaccag ccgcggcgcc aggcgccgtg agcgaggcgg
     1561 tggaacatca gcgcgtggat gccgggataa caggtcagca cctcgagcgt ggagcgcgcg
     1621 gcggggtcgc gctcgcggac gctggccaga tcttctcgga ggcggctgaa catgatggaa
     1681 ggactcccgg atgtgcggtg gaaaaaacac ggcggaccgc gtcgagcggg cgccacgcat
     1741 tcaattcccg agaattttaa tcgactatag cgccccgccg aaagcccgcg ctgcgaccgc
     1801 tccacccgca agacgcggct tgcacgcgtg gcgcgggcgg gcaatgatcg cgcgggatca
     1861 ctccggacct gcgctcatgc tccagctctt ccgccgcctg ctcggcatcc cgcccgcccg
     1921 ccccctggtc gacgagtcgc agtggcggcg ggtcgaagcg aggctgccc
//
LOCUS       DAOQNN010000551         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_49922_length_1965_cov_6.374346, whole genome shotgun sequence.
ACCESSION   DAOQNN010000551 DAOQNN010000000
VERSION     DAOQNN010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49922_length_1965_cov_6.374346"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PKV97_17220"
     CDS             <1..305
                     /locus_tag="PKV97_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNB07679.1"
                     /translation="LFGKQRVWDTEDNNGVLVYLLLAEHRIEIVADRALARQLEPAAW
                     QELVDRLATPCREARFEDALAAAVDCLDQLLRAAFPGAGVTGLASGGGLSDRPVLR"
     gene            524..1030
                     /locus_tag="PKV97_17225"
     CDS             524..1030
                     /locus_tag="PKV97_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNB07680.1"
                     /translation="MASKQEQLNELQKKNLEAAMRLAQMSIENSQRIMEIQVTTAKRL
                     FEEGVQNAKALSAAKDPQGAMEMRASYAQRTTEEMLACARQIAEITAATQAEFGRLVG
                     EQLSSGSTDMFEAMQKMFTGMPIADQNALGAMQTAMDTTRAAFEQMTRASTQAFQAFT
                     QQGGKGRR"
     gene            complement(1158..1697)
                     /gene="moaE"
                     /locus_tag="PKV97_17230"
     CDS             complement(1158..1697)
                     /gene="moaE"
                     /locus_tag="PKV97_17230"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HNB07681.1"
                     /translation="MVEARAQDAAEVSVQHEDFDVGGEIARLGEGRRDVGAVASFVGL
                     VRDANDGSGVRAMTLEHYPGMTERALEEIVEQAKARWSLLSVRVIHRYGRLEPADRIV
                     FVGVTSAHRGEAFAACEFIMDYLKTRAPFWKCEETPQGARWVDARESDDSAAARWAAS
                     GAQVPAGEGEDWDDIELRR"
     gene            complement(1700..1960)
                     /gene="moaD"
                     /locus_tag="PKV97_17235"
     CDS             complement(1700..1960)
                     /gene="moaD"
                     /locus_tag="PKV97_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HNB07682.1"
                     /translation="MTSKVKILYFASLREAVGCAGEELELPAGVATLGALRSHLAARG
                     EGWQALAAGRNVRAALNQRMAGADASLDGGDEVAFFPPVTGG"
BASE COUNT          329 a          716 c          643 g          277 t
ORIGIN      
        1 cgctgttcgg caagcagcgc gtgtgggaca ccgaggacaa caacggcgtg ctggtctatc
       61 tgctgctcgc cgagcaccgc atcgagatcg tcgcggatcg tgcgctcgca cgccagctcg
      121 aacccgccgc ctggcaggag ctggtcgatc gcctcgccac gccctgccgc gaggcgcgct
      181 tcgaggacgc gctcgcggcc gcggtggatt gcctggacca gctgctccgc gcggctttcc
      241 ccggggcggg cgtcaccggc cttgcgagtg gtggcggtct ctccgaccgc ccggtgctgc
      301 gctgaagcgg ccgaagctgt tgcagcgcag catcatacgc acccgtcacg ccttcgaagc
      361 accggatgca ccgcggaacc gcaagcgagc tgtgctattg tggaagccgg gatggtgaag
      421 ccggtgagga tgtgccggcc cgagaacaac aagggacacc gtcccgaggg cgccaagccc
      481 ggaaagagtc agacaaccga tacgtgcgag tggagaggag cctatggcca gcaaacagga
      541 acagttgaac gaacttcaga agaagaacct cgaggcggcg atgcgcctgg cccagatgtc
      601 gatcgagaac tcgcagcgca tcatggagat ccaggtcacc acggccaagc gtctgttcga
      661 ggaaggcgtg cagaacgcca aggctctctc ggccgcgaag gacccgcagg gcgcgatgga
      721 aatgcgcgcg agctacgcgc agcgcaccac cgaagagatg ctcgcgtgcg cacgccagat
      781 tgccgagatc accgctgcca cccaggccga gttcggccgg ctggtggggg agcagctgag
      841 ctcgggcagc accgacatgt tcgaagccat gcagaagatg ttcaccggca tgccgatcgc
      901 cgatcagaac gcgctgggcg cgatgcagac cgcgatggac accacccgtg cggccttcga
      961 acagatgacg cgcgcctcca cccaggcctt ccaggccttc acccagcagg gtggcaaggg
     1021 tcgtcgctga acgcctccgc ctcgtgtggc gggccgttcc cgctgcgatt gaaaacggcg
     1081 ccttgcggcg ccgttttatt tgatccgcat cccgcaagaa cccgcaaagg gcggcctccc
     1141 cgaccgtcct cgccccctca gcgccgcagc tcgatgtcgt cccagtcctc gccctcgccc
     1201 gcggggacct gcgcaccgga ggcggcccag cgcgcagcgg ccgagtcgtc actctcgcgc
     1261 gcatcgaccc aacgcgcgcc ctgcggcgtc tcctcgcact tccagaacgg ggcccgcgtc
     1321 ttcaggtagt ccatgatgaa ctcgcaggcg gcgaaggcct cgccacgatg cgcgctcgtc
     1381 acgccgacga agacgatgcg gtcggcgggc tccaggcggc cgtagcggtg gatgacgcgc
     1441 acggagagca gcgaccagcg cgccttcgcc tgctcgacga tctcctcgag cgcgcgctcg
     1501 gtcatgcccg ggtagtgctc cagcgtcatc gcgcgcacgc cgctgccgtc gttggcgtcg
     1561 cgcaccaggc cgacgaagct cgccaccgcg ccgacgtcgc gccgtccctc gccgaggcgc
     1621 gcgatctcgc caccgacgtc gaagtcctcg tgctggacgc tgacctcggc ggcgtcctgc
     1681 gctctcgctt ccaccatcgt cagcctccgg tcaccggcgg aaagaaggcg acctcgtcgc
     1741 cgccatcgag cgacgcatcg gcgcccgcca tcctctggtt gagcgccgcg cgcacgttgc
     1801 gcccggccgc cagcgcctgc cagccctcgc cgcgcgcggc caggtggctg cgcaaggccc
     1861 ccagcgtggc gacgcccgcg ggcaactcca gttcctcgcc ggcgcagccc accgcctcgc
     1921 gcaggctcgc gaaatacaga atcttcacct tgctcgtcat gtccg
//
LOCUS       DAOQNN010000552         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_50016_length_1963_cov_5.281971, whole genome shotgun sequence.
ACCESSION   DAOQNN010000552 DAOQNN010000000
VERSION     DAOQNN010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50016_length_1963_cov_5.281971"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PKV97_17240"
     CDS             complement(<1..889)
                     /locus_tag="PKV97_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNB07683.1"
                     /translation="MSSLPCRLSDAAASAAAATLLALGGPASAAGVELVDDTGRKLAL
                     AAPAQRIVSLAPHVTEMLFAAGAGERVVGAVDYSDYPEAAQRIARVGGYTRIDLEAVA
                     ALRPDLVIGWQSGNREGDLARLQALGIPVYLSEPRNLEDVARNLERLGQLAGSEPAAQ
                     AAASAFRARREHLAATYSARDKVRVFYQIWDRPLMTVNDHHLIADVIRLCGGANVFGE
                     VAHLTPTIGVEAVLAANPEVIVASGMGEARPEWLDQWSRWPQLEAARRDNLFFIPPEF
                     IQRHTPRILDGAARLCGQVE"
     gene            complement(889..1878)
                     /gene="cobD"
                     /locus_tag="PKV97_17245"
     CDS             complement(889..1878)
                     /gene="cobD"
                     /locus_tag="PKV97_17245"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HNB07684.1"
                     /translation="MLEHGGRLRRAAEHYGIALADWMDLSTGISPWAYPVPPVPETAW
                     QRLPEDDDGLEEAAARYYGSAELLAVAGSQPAIQALPAVLPGARVRLLAPGYAEHAHA
                     WRERRPRSIDAEEVEGAIDDSDVVVLVQPNNPTGVHFDRDRLLDWHARLARRGGWLVV
                     DEAFADTTPAHSLVPLAGRPGLVVLRSLGKFFGLAGARVGFVFAPTEVRTALAERLGP
                     WTLAGPARWAAHHALADTAWQAAQRARLDAAGARLHRLLQAHGLESCGPALFQYVRLP
                     HAAQVHAAFARRGILLRRFDAPPALRFGLPADEPRWARLEAALADCPNLLKEP"
BASE COUNT          269 a          716 c          745 g          233 t
ORIGIN      
        1 tctcgacctg gccgcacagg cgcgccgcgc cgtcgaggat gcgcggcgta tggcgctgga
       61 tgaactcggg cgggatgaag aacaggttgt cgcggcgcgc ggcctcgagc tgcggccagc
      121 gcgaccactg gtcgagccac tccggacggg cctcgcccat gcccgaggcc acgatcacct
      181 cggggttggc cgcgagcacc gcctcgacgc cgatcgtcgg cgtcaggtgg gcgacctcgc
      241 cgaacacgtt ggcgccgcca cagaggcgga tcacgtcggc gatcaggtgg tggtcgttca
      301 ccgtcatcag cgggcgatcc cagatctggt agaaaacgcg caccttgtcg cgtgccgaat
      361 acgtggcggc gaggtgctcg cggcgcgcgc ggaaggcgct cgccgcggcc tgggcggcgg
      421 gttcgctgcc ggcgagctgc ccgagtcgct ccaggttgcg cgccacgtct tccaggttgc
      481 gcggctcgct caggtagacc gggatgccga gcgcctgcag gcgggcgaga tcgccttcgc
      541 ggttgccgct ctgccagccg atcaccaggt ccgggcgcag cgccgcgacc gcctcgaggt
      601 cgatccgggt gtagccgccc acgcgcgcga tgcgctgtgc cgcctcgggg tagtcgctgt
      661 agtccaccgc gcccaccacg cgctcgcccg cgccggcggc gaacagcatc tcggtgacgt
      721 gcggggcgag gctgacgatg cgctgcgccg gcgcggctag cgcgagcttg cggccggtgt
      781 cgtccaccag ctcgacgccc gcggcggacg cggggccgcc gagggcgagc agcgtggccg
      841 ctgcggccga ggccgctgca tcggaaagac ggcaggggag agaactcatc acggctcctt
      901 gaggagattc gggcagtctg cgagggcggc ctccagcctc gcccagcgag gctcgtccgc
      961 gggcaggccg aagcgcagcg cgggcggggc gtcgaagcgg cgcagcagga tgccgcggcg
     1021 ggcgaaggcc gcgtgcacct gcgccgcgtg cggcaggcgg acgtactgga acaaggccgg
     1081 cccgcagctt tccagtccat gcgcctgcag caggcggtgc aggcgcgcgc ccgcggcgtc
     1141 cagccgggcg cgctgcgcgg cctgccaggc ggtgtcggcg agcgcgtggt gcgccgccca
     1201 gcgtgccgga ccggccagcg tccacggccc gaggcgttcg gcgagcgccg tgcggacctc
     1261 ggtgggtgcg aacacgaagc ccacgcgcgc cccggccaga ccgaaaaact tgcccagcga
     1321 gcgcagcacc accaggccgg ggcgaccggc gagcggcacc aggctgtgcg caggcgtggt
     1381 gtcggcgaaa gcctcgtcca ccaccagcca gccgccgcgg cgggcgagcc gggcatgcca
     1441 gtcgagcagg cgatcgcggt cgaaatgcac gccggtcggg ttgttgggct gcaccagcac
     1501 caccacgtcg ctgtcgtcga tcgcaccctc gacctcctcc gcgtcgatcg accgtggccg
     1561 gcgctcgcgc caggcgtggg cgtgctcggc gtagcctggc gccagcaggc ggacgcgtgc
     1621 gccgggcagg accgccggca gggcctggat cgccggctga gaacccgcca ccgcgagcaa
     1681 ttcggcgctg ccgtaatagc gcgcggcggc ttcctccagg ccgtcgtcgt cctcgggcag
     1741 gcgctgccag gccgtctccg gcaccggcgg caccggatag gcccacgggc tgatcccggt
     1801 ggacaggtcc atccagtcgg caagcgcgat accgtagtgc tcggcggcgc ggcgcaggcg
     1861 gccgccgtgc tcaagcaccg ccgcctcccg ccagcgtcgt cgcgacgccg aggaccgcgg
     1921 tcagccccag ccacagcagc gcaccacgcc gcaccagggc tac
//
LOCUS       DAOQNN010000553         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_50327_length_1956_cov_13.117833, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000553 DAOQNN010000000
VERSION     DAOQNN010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50327_length_1956_cov_13.117833"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1747)
                     /locus_tag="PKV97_17250"
     CDS             complement(68..1747)
                     /locus_tag="PKV97_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HNB07685.1"
                     /translation="MFVKITTSGPRQYVKLVESYRDTAGVPRQRIIATLGRIEAVRSG
                     ETDSLLNGLLRAAGKPSLEEGTGEVAFAPALSVGDTWLLTALWKELGFADTFRRLLRN
                     RHQFDAERLLRVMVFNRLCDPESKLGILRWLEGARVPEVSAEAVNHQHLLRTMDTLAE
                     GADRVDDALAGLLRPLIDQELAIVFYDLTTIRAEGSTDESEDLRHVGHAKEGGTVRQV
                     MLGVVQTAEGLPIHHEVFAGNTGETTTLVPTIEKVLARYPIKRVVLVADRGLLSLDNL
                     DAIRALRVGDQPLEFILAVPARRYGDFDSLLAPFHEKSCRLATAEVFGELNWQGFRLI
                     VAHRPDRASEQSRVRDERIAALEADAAQWAEKLDAQEAGQTRPGKKLSDAGTTARFYK
                     AVADAHLAHIIKVDLAAEVFTYAIDERALNRARLMDGKLILVSNVPQFPPAEIVARYK
                     ALADIERGFRVLKSEIEIAPVFHRLPDRIRAHALICFLALLLYRVLRLRLKAKASPLS
                     PERALEIARRIQYHQVTLHQRQSASGLSAMTPQQKELFETVALPEPSARRL"
BASE COUNT          355 a          639 c          611 g          351 t
ORIGIN      
        1 cgcccgagtt cgacagaaat acggctaagt cattgtttgt gcgcaattct ctggttttga
       61 attggcacta caggcgtctg gcggagggct cgggcagggc gacggtttcg aacagctcct
      121 tctgttgggg ggtcatggcg gaaaggccgg aggctgactg gcgttggtgc agcgtgacct
      181 ggtggtactg gatgcggcgg gcaatctcca gagcgcgctc cggggaaagg gggctggcct
      241 tcgccttcag gcgcaggcgc agtacccgat agagcaggag tgccaggaag cagatcaagg
      301 catgcgcccg gatgcggtcc ggcaggcgat gaaagaccgg cgcgatctcg atctcggact
      361 tcagaacccg gaatccccgt tcgatgtcgg cgagcgcctt gtagcgcgcc acgatctcgg
      421 ccgggggaaa ttgcggaacg ttggaaacga ggatcagttt gccatccatc agccgcgcgc
      481 ggttcagcgc acgttcgtcg atcgcgtagg tgaacacctc ggcggcgaga tcgaccttga
      541 tgatgtgcgc cagatgggcg tcagcgaccg ccttgtagaa acgggccgtc gtgccagcgt
      601 ctgacagctt cttgcccgga cgggtctggc cggcctcctg ggcatcgagt ttctcggccc
      661 actgcgcggc atcggcctcc agcgccgcga tgcgctcatc gcgcacgcga ctctgctcgc
      721 tggccctatc ggggcgatgg gcaacgatca gacgaaaccc ctgccagttc agctcaccga
      781 agacctctgc cgtcgccaag cggcaactct tctcgtggaa cggggcgagc aggctgtcaa
      841 aatccccgta gcgccgcgcc gggacggcca gaatgaactc cagcggctga tcgccgaccc
      901 gcagtgcccg gatggcgtcg agattgtcga gactcaacag gccgcgatcg gccaccagca
      961 ccacgcgctt gatcggatag cgcgccaaca ccttctcgat cgtcggcacc agcgtcgtcg
     1021 tctcgccggt attgccggca aagacctcgt gatggatggg caggccttct gccgtctgga
     1081 ccaccccgag catcacctgg cgcaccgtcc cgccttcctt ggcatggcca acgtgccgca
     1141 aatcctcgga ctcgtcggta ctcccttcgg cacggatcgt cgtcaggtcg tagaagacga
     1201 tcgccaactc ctgatcgatc aacggccgca agagacctgc cagagcgtca tccacccggt
     1261 cggcaccctc cgccagggta tccatcgtgc gtagcaggtg ctggtggtta accgcctcgg
     1321 cgctgacctc cggaacccgg gcgccctcca gccagcgcag gatccccagc ttggattccg
     1381 gatcacacag ccggttgaac accatcaccc gcaacagccg ttcggcgtcg aactggtggc
     1441 ggttgcgcag caaccggcga aaggtgtcgg caaagcccag ttccttccac aaggcggtca
     1501 gcagccaggt atcgccgacc gacagcgccg gtgcgaaggc gacctcaccg gtgccttcct
     1561 cgagggaggg cttgccggcc gcccgcagca gaccgttgag caacgaatcc gtctcgccgg
     1621 atcgcaccgc ctcgatgcgc cccaaggtgg cgatgatccg ctggcgaggc accccggcgg
     1681 tgtcccggta ggactcgacg agcttgacgt actggcgcgg gcccgacgtg gtgatcttca
     1741 cgaacatgtc gctactgtat cctcatactg ccatgatcgc aataggcttc tgggcaaatc
     1801 gtgtcactac aaaagtttct tggccgcgcg ccaactcctt gtttttattg cggcccaagc
     1861 cccgtttatg cgtttggcac gcgaaaagtg tcgaactcgg gatgccgccg ctgctcctcg
     1921 tcctctgaaa tcgccgccag cagctctacc aagccg
//
LOCUS       DAOQNN010000554         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_50436_length_1954_cov_6.616640, whole genome shotgun sequence.
ACCESSION   DAOQNN010000554 DAOQNN010000000
VERSION     DAOQNN010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50436_length_1954_cov_6.616640"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PKV97_17255"
     CDS             <1..1137
                     /locus_tag="PKV97_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNB07686.1"
                     /translation="EHDLVVAAVLSGNRNFEARIHPNIRANYLASPPLVVAFALSGRV
                     NIDLDTEPLGTGSDGRPVYLRELWPSSDEIAAALPFATDPASYRRLYADFTRDHDLWN
                     AIPAPTGQTYDWPPSTYIACPPFFAGFGMTPPPASDIHDARALLLLGDSVTTDHISPA
                     GSFRETSPAGRWLRGQGVEKTDFNSYGSRRGNHDVMVRGTFANVRVKNLMLPPNPDGS
                     RVEGGYTLLDGEQTTVFEAASAWIARGVPTIVFAGEEYGTGSSRDWAAKGTALLGVKA
                     VVARSFERIHRSNLVGMGVLPLQFIGEDSWQTLGLDGSERFTLKGIAAGLAPQQQLKL
                     QIERADGSRRSVPLLCRIDTPIEIEYYRHGGILPYVLRAILGGS"
     gene            1236..1625
                     /locus_tag="PKV97_17260"
     CDS             1236..1625
                     /locus_tag="PKV97_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HNB07687.1"
                     /translation="MIPSTPCPCGSGRAFGACCGPVLAGERPAPTAEALMRSRYTAFT
                     LRDTAYLLASWHPSTRPQDLALDEQPPPKWLGLDVRAHRPLADTRAEVEFLARCRIGG
                     RARRLHETSRFVKEDGRWYYVDGDLHD"
BASE COUNT          291 a          766 c          612 g          285 t
ORIGIN      
        1 gagcacgacc tcgtggtggc cgccgtgctg tcgggcaacc gcaatttcga ggcccgcatc
       61 caccccaaca tccgcgccaa ctacctcgcc tcgccgccgc tggtggtggc cttcgcgctc
      121 tccgggcggg tcaacatcga cctcgacacc gagccgctcg gcaccggcag cgacggtcgg
      181 ccggtgtatc tgcgcgagct ctggccgagc tcggacgaga tcgccgccgc actacccttc
      241 gccaccgatc cggccagcta ccgccggctc tacgccgact tcacgcgcga ccacgacctg
      301 tggaacgcga tccccgcgcc caccggccag acctacgact ggccgccctc cacctacatc
      361 gcctgcccgc ccttcttcgc gggcttcggc atgacgcccc cgccggcgag cgacattcac
      421 gacgcacgcg cactgctgct gctcggcgac tcggtgacca ccgaccacat ctcgcccgca
      481 ggttccttcc gcgagaccag cccggccggg cgctggctgc gtgggcaggg tgtggagaag
      541 acggacttca actcctacgg ctcgcgtcgc ggcaaccacg acgtgatggt gcgcggcacc
      601 ttcgccaacg tgcgcgtgaa gaacctgatg ctgccgccca accccgacgg cagccgcgtc
      661 gaaggcggct acaccctgct cgacggcgag cagaccacgg tgttcgaggc ggcgagcgca
      721 tggatcgcgc gcggcgtgcc gaccatcgtg ttcgcgggcg aggaatacgg caccggctcg
      781 tcgcgcgact gggctgccaa gggcaccgcg ctgctcgggg tgaaggcggt ggtggcgcgg
      841 agcttcgagc gcatccaccg ctccaacctg gtcggcatgg gcgtgctgcc gctgcagttc
      901 atcggcgagg acagctggca gacgctgggg ctcgatggga gcgagcggtt cacgctgaaa
      961 ggcatcgcag ccggcctggc gccgcaacag cagctcaagc tgcagatcga gcgcgccgac
     1021 gggtcgcggc ggtcggtgcc cctgctgtgc cgcatcgaca ccccgatcga gatcgagtac
     1081 taccgccacg gcggcatcct gccctacgtg ctgcgcgcga tcctcggcgg aagctgagcc
     1141 gcgcggccgg atcgcaagct gcgtcgtcgc agccccccgc agcacgctcc ggccccgcgt
     1201 tcgcatctcc cctcggcccg ctatactcgc cccccatgat cccctccacc ccttgcccct
     1261 gcggctccgg tcgcgcattc ggggcgtgct gcgggccggt gctcgcgggc gaacgcccgg
     1321 cgcccaccgc cgaggcgctg atgcgctcgc gctacacggc cttcaccttg cgcgacaccg
     1381 cctacctgct cgcgagctgg catccctcca cccgcccgca ggacttggcg ctcgacgagc
     1441 agccgccgcc gaaatggctc ggcctcgacg tgcgtgccca ccgcccgctc gccgacaccc
     1501 gcgcggaggt cgaattcctc gcccgttgcc gcatcggcgg ccgcgcccgg cgcctgcatg
     1561 agaccagccg cttcgtgaag gaggacgggc gctggtatta cgtcgacggc gacctccacg
     1621 actgaggctc gccctcgtgc cagccggcct cggcccggga tcaccgccgg cgcctgagcg
     1681 aaccgcccga tcgcgacttg cgtcgctccc acaaagacca aaacgccccg caatccgcgc
     1741 ggccacccgg cctgctgtcg gcgctgcgcg acatcgcagg tccggcgcgc gcataattgc
     1801 cccatcgtca tcgcgcgcac ccaacggaag atcatgaacc tcgaaaaagt catcttcggt
     1861 ttcttcatcg tgctggcggc cacgctcaac ttcggcttct tcgtcggcga catcgagaac
     1921 cccgcgcacc acgacgtgta cgaacttttc gccg
//
LOCUS       DAOQNN010000555         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_50775_length_1947_cov_6.654863, whole genome shotgun sequence.
ACCESSION   DAOQNN010000555 DAOQNN010000000
VERSION     DAOQNN010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50775_length_1947_cov_6.654863"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKV97_17265"
     CDS             complement(<1..309)
                     /locus_tag="PKV97_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB07688.1"
                     /translation="MTATAPEAVAALRVLVVDDEAPARARLRDLLGDVAGQQPNAIVG
                     MAANGVEALRLLDAAAADVVLADIRMPVMDGVELARHLARLPAPPALVFVTAYDQYAV
                     Q"
     gene            complement(306..1406)
                     /locus_tag="PKV97_17270"
     CDS             complement(306..1406)
                     /locus_tag="PKV97_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB07689.1"
                     /translation="MAESIKQKPPALGAPGLPDFRNLGVMLRVLLAANLFAALTVAIR
                     LDAPARLADELALMAGRVELPLLLSLVLLYFGAPGLSALPVAAGRGVVLVLASLAAIA
                     ASLLAAVPAAEWWRPLGWAWLAAALCLAYFDWRSRRLSPALAEARLIALTARIRPHFF
                     FNCLNGVLGVIRSEPRRAEQALEELADLFRALMQDGRERVTLAEEIELAERYLGLERL
                     RLGERLAVRWEFGGGGGEAEEDGEGGEGGRGGDGGERRTRVPAELLRARMPSLLLQPL
                     LENAVYHGIEPAAAAGEVIVRIARHGKELCIEVDNPVAAQGAHHVGNRMALDNIRERL
                     MLFHDLEARLEIDEADGRYRVCIRLPWEMEAA"
     gene            1503..>1947
                     /locus_tag="PKV97_17275"
     CDS             1503..>1947
                     /locus_tag="PKV97_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNB07690.1"
                     /translation="MTDTTTPAASANAPADASAKAWSGRFSEPVSELVKRYTASVSFD
                     QRMAAQDIRGSLAHARMLARQGIIGTQDLVEIERGMAQIAGEIERGEFAWNLDDEDVH
                     LNIEKRLTALVGDAGKRLHTGRSRNDQVATDIRLWLRDAIDTILGL"
BASE COUNT          301 a          742 c          661 g          243 t
ORIGIN      
        1 ctgcaccgcg tactggtcgt aggcggtcac gaagaccagc gccggaggcg ccggcaggcg
       61 ggcgaggtgg cgggcgagct ccacgccgtc catcaccggc atgcggatgt cggccagcac
      121 cacgtccgcc gccgcggcgt cgagcagtcg cagggcctcg acgccgttgg cggccatgcc
      181 gacgatcgcg ttgggctgct gcccggcgac gtcgccgagc aggtcgcgca ggcgcgcacg
      241 cgccggcgct tcgtcatcga cgacgagcac gcgcagcgcc gcgacagcct cgggtgcggt
      301 tgcggtcatg cggcctccat ctcccagggc aggcggatgc acacccggta gcggccatcc
      361 gcctcgtcga tctccagcct cgcctcgagg tcgtggaaca gcatcaggcg ctcgcggatg
      421 ttatccagcg ccatgcggtt gccgacgtgg tgcgccccct gcgcggcgac cgggttgtcc
      481 acctcgatgc aaagctcctt gccgtggcgc gcgatgcgga cgatcacctc gcccgcggcg
      541 gcggcaggct cgatgccgtg gtagacggca ttttcgagca ggggttgaag cagcagcgag
      601 ggcatgcggg cgcgcagcag ctccgcgggc acgcgcgtac gcctctcgcc gccgtcacca
      661 cctcgtccgc cctccccgcc ttccccgtcc tcttccgcct ctccaccccc tcctccaaac
      721 tcccagcgca ccgcgaggcg ctcgccgagc cgcaggcgct ccagcccgag atagcgctcg
      781 gcgagctcga tctcctcggc cagcgtcacg cgctcgcggc cgtcctgcat cagcgcgcgg
      841 aacaggtcgg cgagctcctc cagcgcctgt tcggcgcggc gcggctcgga gcggatgacg
      901 ccgagcacgc cgttcaggca gttgaagaag aagtgcggcc ggatgcgcgc ggtcagtgcg
      961 atcagccgcg cctcggcgag cgccggcgac aggcgccggc tgcgccagtc gaaatacgcc
     1021 aggcagagcg cagcggcgag ccatgcccag cccagcggtc gccaccactc cgcggccggc
     1081 accgcggcga gcaggctggc ggcgatcgcc gccaggctgg cgagcacgag tacgacgccg
     1141 cgcccggccg cgaccggcag cgcgctcagc ccgggcgcgc cgaagtagag gagcaccagg
     1201 ctcagcagca gcggcagctc cacccgcccg gccatcagcg cgagctcgtc ggcgagccgg
     1261 gcgggcgcat caagccggat cgccacggtg agcgcggcaa agaggttcgc cgccagcagg
     1321 acgcgcaaca tcaccccgag gttgcggaag tcgggcaggc ccggcgcgcc gagcgccggg
     1381 ggcttttgct ttatactttc ggccatctac gcgcgccacc gcaggcggcg ccccttcccg
     1441 taatcgttcc atgccgggcc ggcagcgcag ggccggcctt ttccgtttcc aacaccccga
     1501 ttatgacaga caccacgacg ccggccgcca gcgccaacgc ccccgccgac gcgagcgcca
     1561 aggcctggtc cggccgcttc tccgaacccg tttccgaact cgtcaagcgc tacacggcga
     1621 gcgtgtcctt cgaccagcgc atggccgcgc aggacatccg cggctcgctc gcgcacgcca
     1681 ggatgctcgc ccgccagggc atcatcggca cgcaggatct cgtcgagatc gagcgcggga
     1741 tggcacagat cgccggcgag atcgagcgcg gcgaatttgc ctggaacctc gacgacgagg
     1801 acgtgcacct gaacatcgag aagcgcctca ccgcgctggt gggcgacgcg ggaaaacgcc
     1861 tgcacaccgg ccgcagccgc aacgaccagg tcgccaccga catccgcctg tggctgcgcg
     1921 acgccatcga caccatcctc ggcctga
//
LOCUS       DAOQNN010000556         1941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_51083_length_1941_cov_6.766172, whole genome shotgun sequence.
ACCESSION   DAOQNN010000556 DAOQNN010000000
VERSION     DAOQNN010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51083_length_1941_cov_6.766172"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..>1941)
                     /locus_tag="PKV97_17280"
     CDS             complement(239..>1941)
                     /locus_tag="PKV97_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNB07691.1"
                     /translation="GGILALLLAYAGLKARQRRKEEEIRAAEAALGAASVGSSGQSVF
                     AGTGGQSVDTGATSIMHTDFSHSGLSSIDTDEGVDPVAEADVYMAYGRDAQAEEILQD
                     ALKADPGRAAIHLKLLEIYAQRRSTRQFETVASELFARSGGLGRDWEKAAALGRRLDP
                     ENPLYAAERGDTGAQRAPQGEPAVAPTVAAAAGVAVAGAVATTAEAAPTGPELASLDF
                     TAAAAAQPSHSQMRDTWTLPGELNKFASGEREGPAGEPAGEGAPAAPLADEIPFDTGA
                     LDFELDLADDTPTAMPDLSATTPGLGREFDLTLEAADEEPPPPAPAFIPVSEPAADGG
                     LTFDLDLGEPDEPFEAAAREPQPASPAAIEAASAPEAFASAPTISVEDSGTISNLDFE
                     LPGMEAPGGEAFDLDATVVSATGTLDDELAPPPAAPAGTADEAVVDLEKTAFDSSLLD
                     FDFELDAPATPTQAAVPAGLDLTSIDLDLEALPAEAGAAFDAVPFSVADTQFDQPAAG
                     GAEAPGSMNEEAETKLELARAYEEMGDKEGALELLEEVVAEGTPRQQTTARDMIARLG
                     "
BASE COUNT          262 a          684 c          723 g          272 t
ORIGIN      
        1 atggcgatcg cgatcagcag cacgcggatc cgccgcacga ggcgccaggt gcgcgcgcgc
       61 gcgaagacgg cggccgcgag cgcacaggcc gcgaccgcga cgccgagcgc agcggacggc
      121 aggagctgga cggcggcgac cgccgcgagc cagaggagca ggacaaggcc ggagtgcatc
      181 ggtaaacgga aaaggccgcc gcgagcccgc gctcgtggcg gcctgccggc gggggcgttc
      241 agcccaggcg cgcgatcata tcgcgggccg tggtctgctg tcgcggcgtc ccctcggcga
      301 ccacttcctc gagcaattcg agcgcacctt ccttgtcgcc catctcctcg taggcgcggg
      361 ccagttcgag cttggtctcg gcctcctcgt tcatcgaccc cggcgcttcc gcgccgcctg
      421 cggccggctg gtcgaactgg gtgtcggcca ccgagaacgg caccgcatcg aacgcggcac
      481 cggcttcggc gggcagcgcc tcgaggtcga gatcgatcga ggtcaggtcg agcccggccg
      541 gcaccgcggc ctgggtcggg gtcgccggtg cgtcgagctc gaagtcgaag tcgagcaggc
      601 tggagtcgaa ggcggtcttc tcgaggtcga ccacggcctc gtcggccgtg cccgcagggg
      661 ccgccggcgg cggcgcgagc tcgtcgtcga gcgtgccggt ggcgctgacc acggtcgcat
      721 cgagatcgaa ggcctcgccg cccggagcct ccatgccggg gagctcgaag tcgaggttgc
      781 tgatcgtgcc ggagtcttcc accgagatcg tcggcgcgga ggcgaacgcc tcgggtgcgg
      841 atgcggcttc gatcgcggcg gggctggccg gctgcggctc gcgcgccgcg gcctcgaagg
      901 gctcgtcggg ctcgccgaga tcgaggtcga aggtcagccc gccatcagcg gccggctcgg
      961 aaacgggaat gaaagctggg gccgggggag gcggctcctc gtccgcggct tccagggtga
     1021 gatcgaactc gcgaccgagg ccgggcgtgg tcgccgacag gtcgggcatc gcggtgggcg
     1081 tgtcatccgc gaggtcgagc tcgaagtcga gcgcaccggt gtcgaagggg atctcgtcgg
     1141 ccaggggcgc agccggcgcc ccctctccgg ccggctcgcc tgccggaccc tcacgctcgc
     1201 ccgaggcgaa cttgttcagc tctcccggca atgtccacgt gtcgcgcatc tgcgagtggc
     1261 tgggctgcgc agcggccgcc gcggtgaaat cgagcgaggc cagctccggc ccggtcggtg
     1321 cggcctcggc tgtggtcgcg acggcgcctg cgacagcgac gcccgctgcc gcagcgaccg
     1381 tcggtgccac cgcgggctcg ccctgcggcg cgcgctgcgc acccgtgtcg ccacgctccg
     1441 cagcatagag cggattctcc ggatcgagcc ggcgccccag cgcagcagcc ttctcccagt
     1501 cgcgccccag gccgccggag cgtgcgaaaa gctcgctcgc caccgtctcg aactggcgcg
     1561 tgctgcggcg ctgggcatag atctcgagca gcttgagatg gatcgcggcc cggcccggat
     1621 cggccttgag ggcatcctgc aggatttcct ctgcctgcgc gtcacggccg taggccatgt
     1681 agacgtccgc ctcggccacc gggtccacgc cctcgtcggt atcgatcgag gacagcccgg
     1741 aatggctgaa gtcggtgtgc atgatgctgg tggcaccggt atcgacgctc tggccgccgg
     1801 tgcccgcgaa cacgctctgg ccgctgctgc ccaccgaggc ggcaccgagg gcggcctcgg
     1861 cagcacggat ctcttcttcc ttgcggcgct ggcgcgcctt caggccggcg taggcgagca
     1921 acagcgcgag gatgccacca c
//
LOCUS       DAOQNN010000557         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_51286_length_1936_cov_8.136629, whole genome shotgun sequence.
ACCESSION   DAOQNN010000557 DAOQNN010000000
VERSION     DAOQNN010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51286_length_1936_cov_8.136629"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKV97_17285"
     CDS             <1..452
                     /locus_tag="PKV97_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HNB07692.1"
                     /translation="AQLPPVESLSLSSDFTAFLKEEVSEALRRKALQKLFSDPHFNRM
                     DGLDIYIDDYSQPDPIPPEILAKLQHAREWLMDDATAQPAPEGATALVDAAPVDGERG
                     RGTTAEPAAAEECASAQAATAPGGRCVDDLSGAVSSEPPQESGQNAP"
     gene            581..>1936
                     /locus_tag="PKV97_17290"
     CDS             581..>1936
                     /locus_tag="PKV97_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004384343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB07693.1"
                     /translation="MSERRDPTSRIHLCDCNRSFALDAGRLAAGTDAAMQCHHALCGA
                     ELPALQTSLAGGKTVHVACTQETALFGELAEEAGAGERIRFFNLREHAGWSAESAAAT
                     PKLAALIAAATTLADPEPVAAVQMSAGRSLLIIGEAGAALGWAERLAGSFEPAVLITA
                     RSKDVELPAVNAYPVWSGRPVRLGGHLGAFELEWTQHNPIDLELCVRCNACVKACPEG
                     AIGWDLQVDVDACRSHGACVAACGEIGAIDFARQDSARSERFDLVLDLSAEPLLRRVE
                     LPDGYAAPGRDPFEQALAVQALGEFVGEFEKPRYVAFEAALCAHSRAKKTGCSNCIES
                     CSTAAIRSNGDVIAVDPYLCKGCGTCSTVCPSGALSFQYPRVPELGQRVKTLLAEYAR
                     AGGTDACLLFHSAAAGSAAIARLARRGCGLPARVIPVEVWSADAVGLDLMLGSLALGA
                     CQ"
BASE COUNT          278 a          671 c          692 g          295 t
ORIGIN      
        1 aggcgcaatt gccgccggtc gagagcttgt cgctgtcctc ggatttcacc gccttcctga
       61 aggaagaggt gagcgaggcg ctgcgtcgca aggccttgca gaaactgttc tccgacccgc
      121 acttcaatcg catggacggg ctggacatct acatcgacga ctactcgcag cccgacccga
      181 tcccgccgga gatcctggcg aagctccagc atgcgcgcga atggctgatg gacgacgcga
      241 cggcacagcc cgctcccgag ggggcgacgg cgcttgtaga cgcagcgccc gtcgacggcg
      301 agcggggacg ggggaccacc gcggaaccgg ccgctgcgga ggagtgcgcg tctgcgcaag
      361 ctgcgactgc tccgggtggg cgatgcgtcg acgatctgtc gggcgcggtg tcgtcggagc
      421 cgccgcaaga gtcgggacaa aacgcaccct gagcgacaaa ttgtcgcgcg tcgattccgg
      481 atatcgccca gtcatggctt ttcggggatt cctgcggtct tccctgagtc cccggctggc
      541 ctatgaactg ctttgcgtaa gggaatcgat ggaagaacac atgagcgaac gccgcgaccc
      601 gacctcccgt atccacctgt gcgattgcaa ccgcagcttc gcgctcgacg ccgggcgtct
      661 tgcggcgggc acggatgccg cgatgcagtg tcatcacgcg ctctgcggcg ccgaactccc
      721 ggcgctgcaa acttcgctcg caggcgggaa gaccgtgcat gtcgcctgta cgcaggaaac
      781 cgcgctcttc ggcgagctcg cggaggaagc cggcgcgggc gagcgcatcc gcttcttcaa
      841 cctgcgcgag cacgccggct ggtcggccga gtccgcggcg gcaacgccca agctcgccgc
      901 gctgatcgcg gcggccacca ccttggccga ccccgagccg gtggctgcgg tgcagatgag
      961 cgccgggcgc agcctgctga tcatcggcga ggcgggcgcg gcgctgggct gggcggagcg
     1021 cctggccggc agcttcgagc cggcggtgct gatcaccgcg cgctcgaagg acgtggagct
     1081 gccggcggtc aacgcgtacc cggtgtggtc gggcaggccg gtgcggctcg gcggccacct
     1141 cggcgccttc gagctggagt ggacgcagca caacccgatc gatctcgagc tgtgcgtgcg
     1201 ctgcaacgcc tgcgtcaagg cctgtcccga gggcgcgatc ggctgggacc tgcaggtcga
     1261 tgtcgacgcc tgtcgcagcc acggcgcctg cgtcgccgcc tgtggcgaga tcggcgcgat
     1321 cgacttcgcg cgccaggaca gcgcgcgcag cgaacgcttc gacctcgtgc tcgacctgtc
     1381 ggccgagccg ctgctgcgtc gcgtcgagct gcccgacggc tacgccgcgc ctgggcgcga
     1441 tcccttcgag caggcgctcg cggtgcaggc gctgggggag ttcgtcggcg agttcgagaa
     1501 gccgcgctac gtagccttcg aggccgccct gtgcgcgcac agccgggcga agaagaccgg
     1561 ctgcagcaac tgcatcgagt cctgttccac cgcggcgatc cgctcgaacg gcgacgtgat
     1621 cgcggtcgac ccgtatctgt gcaagggctg cggcacctgc agcacggtgt gtccctcggg
     1681 cgcgctgtcc ttccagtatc cgcgcgtgcc cgagctcggc cagcgcgtga agaccctgct
     1741 cgccgaatac gcgcgcgccg gcggcaccga cgcctgcctg ctgtttcatt ccgccgcggc
     1801 cggcagcgcc gcgatcgccc gcctcgcgcg ccgcggctgc ggcctgccgg cgcgggtgat
     1861 cccggtcgag gtgtggagcg ccgacgcggt cgggctggac ctgatgctcg gcagcctcgc
     1921 gctcggcgcc tgtcag
//
LOCUS       DAOQNN010000558         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_51294_length_1936_cov_5.182881, whole genome shotgun sequence.
ACCESSION   DAOQNN010000558 DAOQNN010000000
VERSION     DAOQNN010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51294_length_1936_cov_5.182881"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..>1936
                     /locus_tag="PKV97_17295"
     CDS             59..>1936
                     /locus_tag="PKV97_17295"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HNB07694.1"
                     /translation="MRATLAARDAPRWAGQTSAEREAADLQAVQGVSGRTALSDALRD
                     AAEHYSPDPVTAGRAEQLVFGLTRGVGKALGYGLTMGPMGVAAFAGDEGLAEVDRLQR
                     QGVDDATAHQAGVATTLATGAGALLPVAPLAKGYTAANVAKVAALGAVSGPAAFAAQQ
                     QMTRDILSRAGYDKIAEQSDPLDPWGLATAAVVPWIFGGLSLRGGSRTAGKAPPGRPE
                     APPRAAGAEGVGEAAARAEAPLVPREAVDAAMVHNLTLLRDRAGQIPPEPGLSAAERS
                     VEDTFRSQVLNDPDAALRAYAELPDTAGGKIVNTDEARELSADYAASRDSRALLARAV
                     HGPASYIARLQWDRLLAEPPKTGEVLILGGGGGSGKTSSLHEVMPGFVDRFDAIYDTT
                     LANPRKAGEVIEQALSTGRKVTISFTARDPFDAIANGVIPRAQKKGRTVPLDIAAQAH
                     VEAPQTFAALAAKYADDPRVKVRLVDNTGPEGSAKGIDLQDFPRFDYNDLEGRAMSEA
                     LRAFKEGAIDETVFRGLTGRREGEGGAQDSRGLPGRPSGSGGSGVGGQPEPQHGAGSA
                     GARGAQAPTGTAEGGLTAAAVAPEGAGLPTLAELTRASANASTLAAAVERAGAGAAGL
                     LR"
BASE COUNT          302 a          673 c          734 g          227 t
ORIGIN      
        1 ttgcccgggc cacgcgtcga cgcgcaccac gtgcggcgcc gagacggcca cggctgtcgt
       61 gcgcgcgacg ctggccgcgc gcgacgcgcc gcgctgggcc gggcagacca gcgccgagcg
      121 cgaggcggcc gatctgcagg cggtgcaggg ggtgagcggg cgcactgcac tcagcgatgc
      181 gctgcgcgac gcggccgagc actacagccc cgacccggtg acggcgggca gggcagagca
      241 gctcgtcttc ggcctcacgc gcggggtcgg caaggcgctc ggctacggcc tgacgatggg
      301 gccgatgggc gtggcggcct tcgccggcga cgagggcctg gctgaggtcg accggctgca
      361 gcgccagggc gtggacgacg ccacggcgca ccaagctggc gtcgccacga cgctcgccac
      421 gggcgccggc gcgctcttgc cggtggcgcc gctggccaag ggctacacgg cggccaacgt
      481 ggccaaggtg gcggcgctgg gcgccgtgtc cgggccggct gcgttcgccg cgcagcagca
      541 gatgacgcgc gacatcctct cgcgcgccgg gtacgacaag atcgccgagc aaagtgatcc
      601 gctggacccg tgggggctcg cgacggctgc ggtggtgccg tggatcttcg gcggcctgtc
      661 gctgcgcggc ggctccagga cggccggcaa ggccccgccg gggcggccag aagccccacc
      721 acgggctgcg ggggccgagg gggtgggtga ggccgccgcg cgcgccgagg cgccgcttgt
      781 gccgcgggaa gccgtcgacg cggcgatggt gcacaacctg acgctgctgc gcgaccgcgc
      841 ggggcagata ccgccggagc ccggcttgag cgccgccgag cgatccgtcg aagacacctt
      901 ccgctcgcag gtgctcaacg accccgacgc ggcgctgcgc gcgtacgccg agttgccaga
      961 caccgccggc ggcaagatcg tcaacaccga cgaggcgcgc gaactgtctg ccgactacgc
     1021 agcaagtcgc gactcgcgcg cgctgctcgc ccgcgccgtg cacggccctg cgtcctacat
     1081 cgcgcggctt cagtgggatc ggctgctcgc ggaaccgccc aagaccggcg aggtgctgat
     1141 cctcgggggc ggtggcggct cgggcaagac gagcagcctg cacgaggtga tgcccgggtt
     1201 cgtcgaccgc ttcgatgcga tctatgacac gacgcttgcc aacccgcgca aggcggggga
     1261 ggtgatcgag caagcgctct cgacagggcg caaggtgacg atcagcttca ccgcccgcga
     1321 cccgttcgat gcgatcgcca acggcgtgat ccctcgcgcg cagaagaagg gccgcacggt
     1381 gccgctcgac atcgccgcgc aggcgcacgt cgaagcgccg cagacgttcg cagcgcttgc
     1441 tgccaagtac gccgacgacc cgcgcgtgaa ggtgcgactt gtggacaaca ccggcccgga
     1501 gggctcggcc aagggcatcg atctacagga tttccctagg ttcgactaca atgacctcga
     1561 gggccgagcg atgagcgaag ccctgagggc gttcaaggaa ggggcgatcg atgaaacagt
     1621 ttttcgaggc cttaccggac gacgtgaggg cgaaggtggc gcgcaagatt cacgagggct
     1681 acctggacgc ccttcaggat ccggcggctc aggagttggt ggacaacctg aaccgcaaca
     1741 cggtgcggga agcgcgggag cgcgaggcgc acaagcccca accggcaccg ctgaaggcgg
     1801 cctgaccgct gcggccgtcg cgccagaggg cgcggggctg cccaccctcg ccgagctgac
     1861 cagggccagc gccaacgcca gcaccctggc ggccgccgtc gagcgcgctg gcgcaggcgc
     1921 cgcagggctg ctgcgc
//
LOCUS       DAOQNN010000559         1931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_51556_length_1931_cov_14.158849, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000559 DAOQNN010000000
VERSION     DAOQNN010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51556_length_1931_cov_14.158849"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1008
                     /gene="ilvE"
                     /locus_tag="PKV97_17300"
     CDS             76..1008
                     /gene="ilvE"
                     /locus_tag="PKV97_17300"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR01122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HNB07695.1"
                     /translation="MLAEPAPQPRPSFEQAVCWMNGRLMPAAEATVSVFDHGLLYGDG
                     VFEGIRFYHRRAFRLQAHLARLTLSARALALSLPWDQAALTRAVAETITAAPDDAGYL
                     RLVVTRGVGALGIDPRSCRQPNLFIIADRLDAMAEARRRGARLIIAATRRLGADGLDP
                     RIKSLNYLNPILARLEANRAGADEAVMLNAAGRVTEGSADNLFIVQGGRLLTPPASDG
                     ALAGITRAVVLELAATLGIAACEQPLTPYDLYTADECFLTGTAAELVPVADIDGRPLS
                     HCPGPLFSRLDQAFRALIERECRNKPPAVTGEAE"
     gene            1005..1394
                     /locus_tag="PKV97_17305"
     CDS             1005..1394
                     /locus_tag="PKV97_17305"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB07696.1"
                     /translation="MIDATFAQRFAADWIAAWNRHDLDAILAHYADDFEMVSPLIPQL
                     AGEPSGRLRGKTRVGAYWARALTPPALTLSPRLRFELIGILTGVDSVALHYRSQRGPA
                     VEVLQFGPDGKVVRAAAHYLPQTQEKS"
     gene            1391..1882
                     /locus_tag="PKV97_17310"
     CDS             1391..1882
                     /locus_tag="PKV97_17310"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB07697.1"
                     /translation="MSNDTPPVALRAADCPPRTRPSNYPPAIRNWLDGKLAGREKRPL
                     GDPFGLTHFGVNLTRLAPGAISALRHAHTLQDEFIYVLEGTPTLITDAGETPLAPGMC
                     AGFRAGSGDAHQLINRSDTDVLYLEIGDRTPGDSAAYPDDDLAAELIDGRWHFTHKDG
                     TAY"
BASE COUNT          305 a          703 c          636 g          287 t
ORIGIN      
        1 ctggcggcgc gccgcgctcg cgcccgccca agccaaagag tttcgatgaa cgtgcccacc
       61 acaggagaga cgaagatgct tgccgaaccc gcaccgcaac cccgcccttc gttcgaacag
      121 gctgtctgct ggatgaacgg tcgcctgatg cccgccgccg aggcgaccgt gtcggtgttc
      181 gaccatgggc tgctctatgg cgatggcgtc ttcgagggca tccgcttcta ccatcggcgt
      241 gccttccggc ttcaggcgca tctggcgcgc ctcacgctgt cggcgcgggc actggcgctg
      301 agcctgccct gggatcaggc ggcgctgacc cgcgcggtgg cagagaccat taccgccgcg
      361 cccgacgatg ccggctatct gcgcctggtg gtgacgcgcg gcgtgggcgc gctggggatc
      421 gacccgcgca gttgccggca gcccaatctc ttcatcattg ccgatcggct cgacgcgatg
      481 gccgaggcgc gccggcgcgg cgcgcggctg atcatcgcgg cgacccgacg gttgggcgcc
      541 gacgggctcg atccgcgcat caagagcctg aactatctga acccgattct ggcgcggctg
      601 gaggccaacc gcgccggcgc ggatgaggcg gtgatgctca acgccgccgg acgggtcacc
      661 gagggcagcg ccgacaatct gttcatcgtg cagggcggcc gcctgctgac gccgccggcg
      721 agcgacggcg cgctggccgg tatcacccgc gccgtggtgc tggagctggc ggcgacgctc
      781 ggcatcgcgg catgcgagca gccgctgacg ccgtatgacc tctacaccgc ggacgaatgc
      841 tttttgaccg gcaccgccgc cgagctggta ccggtggccg acatcgacgg ccggccgctc
      901 agccattgcc cgggaccgct gttcagccgg ctcgatcagg cctttcgcgc gctgatcgag
      961 cgcgaatgcc gtaacaagcc accagcagtc acaggagaag ccgagtgatc gacgctacct
     1021 ttgcccaacg cttcgccgcc gactggatcg ctgcctggaa ccgtcacgat ctcgatgcca
     1081 tcctcgccca ctacgcggat gacttcgaga tggtctcacc actgatcccg caactggccg
     1141 gcgagccctc cggacgactg cgcggcaaga cccgcgtcgg cgcctactgg gcgcgcgccc
     1201 tgacgccgcc ggcgctcacg ctgtcgcccc gcctgcgctt cgagctgatc gggatcctca
     1261 ccggcgtcga cagcgtggcg ctgcactacc gcagccagcg cggcccggcc gtggaggtgt
     1321 tgcagttcgg cccggacggc aaggtcgttc gcgccgcggc ccactacctg ccccaaaccc
     1381 aggagaaatc atgagcaacg acacaccgcc cgtggcactg cgcgccgccg actgcccacc
     1441 ccgcacgcgg ccgtcgaact acccacccgc catccgcaac tggctcgatg ggaagctcgc
     1501 gggccgggag aagcgcccgc tcggcgatcc gtttggcctc acccacttcg gcgtcaacct
     1561 gacgcggctg gcaccgggcg cgatctcggc gctgcgccac gcccacacgc tgcaggatga
     1621 gttcatctat gtgctggagg gcacgccgac gctcatcacc gatgccggcg agaccccgct
     1681 cgcgccgggg atgtgcgccg gttttcgtgc cggctccggt gacgcgcacc agttgatcaa
     1741 ccgcagcgac accgacgtgc tctacctcga aatcggcgac cgcacgccgg gtgacagcgc
     1801 cgcctatccg gacgatgatc tggccgccga gttgatcgat ggccggtggc acttcacgca
     1861 caaggacggc acggcgtact gaagtggcgc aagctcagca atgcagggtg gacaaagcgc
     1921 agcgtgccca c
//
LOCUS       DAOQNN010000560         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_51711_length_1928_cov_6.522157, whole genome shotgun sequence.
ACCESSION   DAOQNN010000560 DAOQNN010000000
VERSION     DAOQNN010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51711_length_1928_cov_6.522157"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PKV97_17315"
     CDS             <1..856
                     /locus_tag="PKV97_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNB07698.1"
                     /translation="VGLIGGSFALALRRAGAVERIVGIGRRREVLERACALGVIDEIA
                     EGWADALDGADLVLLAAPVGQTDAILAAMAPHLRPGTIVTDAGSTKRDVVAALRTHLG
                     GALADVVPAHPIAGAEKSGVEAAFAELYMGRKVVLTPLPESRPEAVQKVRDAWEACGA
                     NVVGMTPQEHDRVFAAVSHLPHLLAFGLVDDLAGRSNAPLLFSHAASGFRDFTRIAGS
                     HPEMWRDICVANRVALLEELDAYLGELARLRMMLVEGDGDGLEAVFERARRARNAWAE
                     GLPIRTAE"
     gene            908..>1928
                     /gene="aroA"
                     /locus_tag="PKV97_17320"
     CDS             908..>1928
                     /gene="aroA"
                     /locus_tag="PKV97_17320"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNB07699.1"
                     /translation="MEFLDLPPMLGAAGSVRLPGSKSISNRVLLLAALAEGETDIRDL
                     LLSDDVERMLEALRALGVDWRREGDSLDYRVCGVGGPFPVKTGDLFLGNAGTAFRPLT
                     AALALSGGEYRLSGVPRMHERPIGDLVDALRQLGADITCTANEGYPPLHLKPATIRPG
                     GVVRVRGDVSSQFLTALLMALPLTGVETTIEVVGELISKPYIRITLELMARFGVQVGQ
                     QGWERFVVPGGARYRSPGTVFVEGDASSASYFLAAGAIGGGPVRVEGVGRTSIQGDVR
                     FAEALEQLGARITLGDNWIEAEAPAGGVLKAFDLDLNHIPDAAMTLAVAALFADGPCR
                     LRNIAS"
BASE COUNT          239 a          698 c          701 g          290 t
ORIGIN      
        1 ggtcgggctg atcggcggct ccttcgcgct cgcgctgcgg cgcgcgggcg cggtcgagcg
       61 catcgtcggc atcggccgcc ggcgcgaagt gctcgagcgc gcgtgcgcgc tcggcgtgat
      121 cgacgagatc gccgagggct gggcggatgc gctcgacggc gccgacctgg tgctgctcgc
      181 cgcgccggtg gggcagaccg acgcgatcct cgccgcgatg gcgccgcacc tgcgccccgg
      241 caccatcgtc accgacgccg gcagcaccaa acgcgacgtg gtcgccgcgt tgcgcaccca
      301 tctgggtggc gcgctcgccg acgtggtgcc ggcgcacccg atcgccggcg ccgagaagag
      361 cggggtcgag gccgccttcg ccgagctcta catgggccgc aaggtggtgc tgacgccgct
      421 gcccgagagt cgccccgagg ccgtccagaa ggtgcgcgac gcgtgggagg cctgcggtgc
      481 caacgtggtc ggcatgacac cgcaggagca cgaccgcgtc ttcgccgcgg tgagccacct
      541 gccgcacctg ctcgccttcg gcctggtcga cgacctcgcc gggcgcagca acgcgccgct
      601 gctgttctcg catgcggcga gcggtttccg cgacttcacc cgcatcgccg gcagtcatcc
      661 cgagatgtgg cgcgacatct gcgtcgccaa ccgggtcgcg ctgctcgagg aactcgatgc
      721 ctacctcggc gagctcgcgc gcctgcgcat gatgctggtc gagggcgatg gcgacggcct
      781 ggaggcggtg ttcgagcgcg cccggcgcgc gcgcaacgcc tgggccgagg ggctgccgat
      841 ccgtaccgcc gagtgagcct gcgctttttc ctgtttcgcg gggacggccc cgttttctga
      901 ggtcatgatg gaatttctcg atctgccccc gatgctgggc gccgccggca gcgtccgcct
      961 gcccggctcg aagagcatct ccaaccgcgt gctgctgctg gccgcgctcg ccgagggcga
     1021 gaccgacatc cgcgacctgc tgctgtcgga cgacgtcgaa cgcatgctcg aggccctgcg
     1081 cgcgctcggc gtcgattggc ggcgcgaggg cgacagcctg gattaccgtg tatgcggcgt
     1141 cggcggcccc ttcccggtca agaccggcga tctcttcctc ggcaacgccg gcaccgcctt
     1201 ccggccgctc accgcggcgc tcgcgctgtc gggcggcgag taccgcctgt cgggcgtacc
     1261 gcgcatgcac gagcgcccga tcggcgacct cgtcgacgcg ctgcgccagc tcggcgccga
     1321 catcacctgc accgccaacg agggctaccc tccgctgcat ctcaagcccg ccacgatccg
     1381 tcccggtggc gtggtgcgcg tgcgtggcga cgtctccagc cagttcctca ccgcgctgct
     1441 gatggcgctg ccgctgaccg gagtggagac cacgatcgag gtggtgggcg agctcatctc
     1501 caagccctac atccgcatca ccctcgagct gatggcgcgc ttcggcgtgc aggtcgggca
     1561 gcagggctgg gagcgcttcg tggtgccggg cggcgcgcgc taccgcagcc cgggcacggt
     1621 gttcgtcgag ggcgacgcct cgtcggcatc ctatttcctc gccgccgggg cgatcggtgg
     1681 tgggccggtg cgggtggaag gcgtggggcg gaccagcatc cagggcgacg tgcgcttcgc
     1741 cgaggcgctg gagcaacttg gcgcgcgcat caccctgggc gacaactgga tcgaggccga
     1801 ggcgcccgcc ggcggcgtgc tgaaggcctt cgacctcgac ctcaaccaca tccccgacgc
     1861 ggcgatgacg ctggcggtgg cggcgctgtt cgccgacggg ccgtgccggc tgcgcaacat
     1921 cgcgagct
//
LOCUS       DAOQNN010000561         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_51956_length_1923_cov_6.651499, whole genome shotgun sequence.
ACCESSION   DAOQNN010000561 DAOQNN010000000
VERSION     DAOQNN010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51956_length_1923_cov_6.651499"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /locus_tag="PKV97_17325"
     CDS             complement(<1..1889)
                     /locus_tag="PKV97_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB07700.1"
                     /translation="MNDPQSLGLDFVADDTLSGFRLQRLEVLNWGTFDRHVWTLQLDG
                     RNGLLTGDIGSGKSTLVDAITTLLVPAHRIAYNKAAGADTRERSLRSYVLGHYKSERN
                     EVTGAARPIGLREPGSYSVILGVFHNAGYDQTVSLAQVFWFKEAQGQPARFYLGAEHA
                     LSITDDFSGFGPDITQLRKRLRGAGAEVFDSFPPYAAWFRRRFGIDNEQALELFHQTV
                     SMKSVGNLTDFVRHHMLEPFEVGPRIQALIGHFDDLDRAHQAVLKAQRQVSLLAPLVE
                     DCHRHDALAADIGTLRRCREALQPHFAARKLALLDHRLELAAEEWARADAQVMRLDTL
                     REEQAGRIDALKQAINANGGDRLERLAAEIRKLAQVRDARLAKSRRYAELAAVLGTPA
                     AADGEAFAAQRLHFAAGREDARNRDAELQNTLTEHAVSLRQGKLEHDALSAEIDSLRR
                     RRSNIDDRQIQIRAALCGALGIDVEDMPFAGELLQVREDERDWEGAAERLLRGFGLAL
                     LVPDAHYKAVAEWVDGNHLRGRLVYFHVRPPRAGELPALHPDSLVRKLAIKPDSPHYD
                     WLERELAHRFDVACCATQEQFRRETRAITRAGQIKDPSGRHEKDDRHAIADRSRYVLG
                     WSNTA"
BASE COUNT          237 a          611 c          777 g          298 t
ORIGIN      
        1 ttggcggtgt tgctccagcc cagcacgtag cggctgcggt cggcgatggc gtggcggtcg
       61 tccttctcgt ggcggccgct ggggtccttg atctggccgg cgcgggtgat cgcgcgcgtc
      121 tcgcggcgga actgctcctg cgtggcgcag caggcgacgt cgaagcggtg ggcgagctcg
      181 cgttccagcc agtcgtagtg cgggctgtcg ggcttgatcg cgagcttgcg caccagcgag
      241 tcggggtgca gcgcgggcag ctcgccggcg cgtggcgggc ggacgtggaa atacaccagg
      301 cggccgcgca ggtggttgcc gtccacccac tcggcgaccg ccttgtagtg cgcgtcgggc
      361 accagcaggg cgaggccgaa gccgcgcagc aggcgctcgg cggcgccctc ccagtcgcgc
      421 tcgtcctcgc gcacctgcag cagctcgccg gcgaagggca tgtcctcgac gtcgatgccg
      481 agcgcgccgc acagcgcggc gcggatctgg atctggcggt cgtcgatgtt gctgcgccgg
      541 cgcctgaggc tgtcgatctc ggcgctcagc gcgtcgtgtt cgagcttgcc ctggcgcagg
      601 ctgacggcgt gctcggtgag cgtgttctgc agctcggcgt cgcggttgcg cgcgtcctcg
      661 cggccggcgg cgaagtgcag gcgctgggcg gcgaaggctt cgccgtcagc ggcggcgggg
      721 gtgccgagca ccgcggcgag ctcggcgtag cggcgcgact tggccaggcg cgcgtcgcgc
      781 acctgcgcca gcttgcggat ctcggcggcg aggcgctcga ggcggtcgcc gccgttggca
      841 ttgatcgcct gcttgagcgc gtcgatgcgg ccggcctgtt cctcgcgcag cgtgtcgagg
      901 cgcatgacct gggcgtcggc acgcgcccac tcctcggcgg cgagttcgag gcggtggtcg
      961 agcagggcga gcttgcgcgc ggcgaagtgc ggctgcagcg cctcgcggca gcgccgcagc
     1021 gtgccgatgt cggcggcgag cgcgtcgtgg cggtggcagt cctcgaccag cggggcgagc
     1081 agcgacacct ggcgctgggc cttgagcacg gcctggtggg cgcggtcgag gtcgtcgaag
     1141 tggccaatca gggcctggat gcgcgggccg acctcgaagg gctcgagcat gtggtggcgc
     1201 acgaagtcgg tgaggttgcc gaccgacttc atcgacacgg tctggtggaa gagctcgagc
     1261 gcctgctcgt tgtcgatgcc gaagcggcgg cggaaccacg ctgcgtaggg cgggaagctg
     1321 tcgaacacct cggccccggc gccgcgcagg cgcttgcgca gctgcgtgat gtcggggccg
     1381 aagccggaga aatcgtccgt gatcgacagc gcgtgctcgg cgccgaggta gaagcgcgcc
     1441 ggctggccct gcgcctcctt gaaccagaac acctgggcca ggctcacggt ctggtcgtag
     1501 ccggcgttgt ggaacacgcc gaggatcacc gagtagctgc cgggctcgcg caggccgatc
     1561 gggcgcgcgg cgccggtgac ctcgttgcgc tcggacttgt agtggccgag cacgtaggag
     1621 cgcaggctgc gctcgcgggt gtcggcgccg gcggccttgt tgtaggcgat gcggtgggcg
     1681 gggacgagca gggtggtgat ggcgtcgacc agggtggact tgcccgagcc gatgtcgccg
     1741 gtgagcagcc cgttgcggcc gtcgagctgc agcgtccaca cgtggcggtc gaaggtgccc
     1801 cagttgagca cctccaggcg ctgcaggcga aagccggaga gggtgtcgtc ggcgacgaag
     1861 tcgaggccga gggattgcgg gtcgttcatg ggctgcacct gcgctgcgca tacgcgccat
     1921 cga
//
LOCUS       DAOQNN010000562         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_52048_length_1921_cov_9.017685, whole genome shotgun sequence.
ACCESSION   DAOQNN010000562 DAOQNN010000000
VERSION     DAOQNN010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52048_length_1921_cov_9.017685"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="PKV97_17330"
     CDS             complement(<1..1446)
                     /locus_tag="PKV97_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB07701.1"
                     /translation="MSAPLTPPAPLPVEHLVARCDPASLGIETSAELPELDVARLHGR
                     AVDAIRLGLDIRAEGYNLFVLGDPGSGRHELVRRLLEDTRGRGDAPADWCYAWNFANA
                     AQPRLLRLPCGRGPALRDDLARFVEELVPAIGAVFESEEHRNRIEALQEEAKTREESA
                     LRSLGDEAQKLGVALLRTPHGFAFLPMKDEGSTLTQEEFEKLPEARQHELGEHIRALH
                     ERLHRLMGDFPRWRHELQNRIRDAGREAIRATVTHMVDELKARHADLPEVCAHLDAVL
                     ADVVASGESLRATPHADEDSETLTYTGSISVQRYLVNLLVANPADGTRPMVYEDHPTL
                     QNLVGRIDHLVHMGTLVSNFTLIRAGALHRANGGFLVLDALKLLSQPFAWEGLKRALK
                     SARLRIESLSELIGVTGSVQLEPEPMPLELKVVLIGDRLTYYLLGRYDPEFAALFRIN
                     ADMESEIERSADNTAAYACLLATLARRAGLPV"
     gene            complement(1625..>1921)
                     /locus_tag="PKV97_17335"
     CDS             complement(1625..>1921)
                     /locus_tag="PKV97_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07702.1"
                     /translation="IGADEFAAGAEISAGIDAVAHDGSALAAEWAVHAEATDLRKGGD
                     AETPPTRIDVRVLHADAASVRFVAPAQPGAYRLFITVRDREGKAGTANLPFRVR"
BASE COUNT          256 a          632 c          757 g          276 t
ORIGIN      
        1 gaccggcagg ccggcgcggc gcgccagggt ggcgagcagg caggcgtaag cggcggtgtt
       61 gtcggccgag cgctcgatct cgctctccat gtcggcgttg atgcggaaga gcgcggcgaa
      121 ctcgggatcg taccggccga gcaggtagta ggtcaggcgg tcgccgatca gcaccacctt
      181 gagctcgagc ggcatcggct cgggctcgag ctgcaccgag ccggtcaccc cgatcagctc
      241 cgagagcgac tcgatgcgca ggcgcgcgga tttgagcgcg cgcttgaggc cctcccaggc
      301 gaagggctgg gacagcagct tgagcgcatc gagcaccagg aagccgccgt tggcgcggtg
      361 cagggcgccg gcgcggatca gcgtgaagtt gctcaccagg gtgcccatgt gcaccaggtg
      421 gtcgatgcga ccgaccaggt tctgcagcgt ggggtggtct tcatacacca tcggtcgggt
      481 gccgtcggcg gggttggcga cgagcaggtt caccagatag cgctgcacgc tgatgctgcc
      541 ggtgtaggtc agcgtctcgc tgtcctcgtc ggcgtgcggc gtcgcgcgca gcgactcgcc
      601 gctggcgacg acgtcggcga gcaccgcgtc cagatgcgcg cacacctcgg gcaggtctgc
      661 gtgacgcgcc ttcaattcgt cgaccatgtg ggtgacggtg gcgcggatcg cctcgcgccc
      721 ggcgtcgcgg atgcggttct gcagctcgtg ccgccagcgc gggaaatcgc ccatcaggcg
      781 gtgcaggcgc tcgtgcagcg cgcggatgtg ctcgccgagc tcgtgctggc gcgcctcggg
      841 cagcttctcg aactcctcct gcgtgagcgt gctgccctcg tccttcatcg gcaggaaggc
      901 gaagccgtgc ggggtgcgca gcagcgcgac gccgagcttc tgggcctcgt cgccgaggct
      961 gcgcagggcg ctctcctcgc gcgtcttggc ctcctcctgc agggcctcga tgcggttgcg
     1021 gtgctcctcg ctctcgaaca ccgcgccgat cgccggcacc agctcctcga cgaagcgggc
     1081 cagatcgtcg cgcagcgccg gaccgcgccc gcagggcagg cgcagcagcc gaggctgggc
     1141 ggcgttggcg aagttccatg cgtagcacca gtccgcgggt gcgtcgccgc ggccgcgcgt
     1201 gtcctcgagc aggcggcgga caagctcgtg gcggccgctg ccggggtcgc cgagcacgaa
     1261 gaggttgtag ccctcggcgc ggatgtcgag gccgagccgg atggcgtcga ccgcgcggcc
     1321 gtggaggcgg gcgacgtcga gttcgggcag ctcggcggag gtctcgatgc cgagcgacgc
     1381 aggatcgcag cgggccacca ggtgctcgac gggcaagggg gccggcggcg tgagcggagc
     1441 ggacatgggc gcgcctcctg cgttgggggt tcttgtgcga gtcatcttaa ccccggggcg
     1501 gggcggcgga ccaagcgggg agaaatgcgc ggggcgggca ccgtgcgccc acgggaggag
     1561 ggggcacgaa cgtgcgagaa ccgcaggcgc gagggagacg acgctgctgc gggcgaggcc
     1621 tcgtttacct cacccggaac ggcaggttcg ccgtccctgc cttgccctcg cggtcgcgca
     1681 cggtgatgaa gaggcggtag gcgccgggct gcgccggggc gacgaagcgc acgctggcgg
     1741 catcggcgtg cagcacgcgc acgtcgatgc gcgtgggcgg ggtctcggca tcaccgccct
     1801 tgcgcaggtc ggtggcctcg gcgtgcacgg cccactcggc ggcgagcgcg ctgccgtcgt
     1861 gggcgaccgc gtcgatgccg gcggagatct ccgcgccggc ggcgaactcg tccgcgccga
     1921 t
//
LOCUS       DAOQNN010000563         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_52641_length_1910_cov_7.083558, whole genome shotgun sequence.
ACCESSION   DAOQNN010000563 DAOQNN010000000
VERSION     DAOQNN010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52641_length_1910_cov_7.083558"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKV97_17340"
     CDS             <1..462
                     /locus_tag="PKV97_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNB07703.1"
                     /translation="LGVPLIVSHMFVFYFGIMADLTPPVALAAFAAAPIAKESGLKIG
                     VRAVQIAIAGFIVPYMAVYAPELMLQGDKGFEATLYVVFKALLAVTLWGTAVVGYFRS
                     PMNPLERVLAFVAASMLVVALPITDELGFGLAALVLGWHIWRSRDTQALTV"
     gene            462..845
                     /locus_tag="PKV97_17345"
     CDS             462..845
                     /locus_tag="PKV97_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HNB07704.1"
                     /translation="MAVCLATSVAAAVVAANAFTLAWTHSIERVRWEEAWRVEDGGLV
                     LERVRVRGHGAGMEPAEGAVLRDGAWEWHPRSRHAVLRLTRSAYTADYEWCADGESCR
                     PLSALIASDGGVTELRACPGGEAVR"
     gene            complement(865..1413)
                     /locus_tag="PKV97_17350"
     CDS             complement(865..1413)
                     /locus_tag="PKV97_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNB07705.1"
                     /translation="MNTLPAGELLALFLHFLTLSLLSIGGAMSTAPEMHRYFVVERGW
                     IGDADFTTAIALAQAAPGPNVLFVPVLGFQAAGLPGAAAALVGILLPSTLLSLGVSRW
                     GASRRDTPLVRAFTAGLAPVTVGLVFATGWLLALPFLGDPAHRNGALVLITLTALVTL
                     RTKLAPIWMILVGALLGAAGIA"
     gene            complement(1410..>1910)
                     /locus_tag="PKV97_17355"
     CDS             complement(1410..>1910)
                     /locus_tag="PKV97_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNB07706.1"
                     /translation="QRELVDRRGWLSRAEFVELYSIAQMLPGPNVVNLSLMIGDRYFG
                     WRGALAAITGMLLAPFLIVIALAASYERLAEYPAVAGALRGMGAVAAGLILAMAFKML
                     GTLRGNLLGPWLCAALGLASLVAIAVFRLPLAWIVIGLGLLGWGLARWRVTVLDARAA
                     HGEEMR"
BASE COUNT          276 a          672 c          706 g          256 t
ORIGIN      
        1 ctcggcgtgc cgctgatcgt ctcgcacatg ttcgtgttct atttcgggat catggccgac
       61 ctcaccccgc cggtcgcgct cgccgccttc gccgcggcgc cgatcgccaa ggagagcggg
      121 ctgaagatcg gcgtgcgcgc ggtacagatc gccatcgccg gcttcatcgt gccctacatg
      181 gcggtgtatg cgcccgagct catgctgcag ggcgacaagg gcttcgaggc gacgctgtac
      241 gtggtcttca aggccctgct cgccgtcacg ctgtggggta cggcggtggt cggctacttc
      301 cgctcgccga tgaacccgct cgagcgcgtg ctcgcctttg tcgccgcgtc catgctggtg
      361 gtcgcgctgc cgatcaccga cgagctcggc ttcggcctgg cggcgctggt gctgggctgg
      421 catatctggc gctcgcgcga cacacaggcg ctcaccgtct gatggcggtc tgcctcgcca
      481 ccagcgtcgc tgccgcggtg gtcgccgcaa acgccttcac cctggcgtgg acgcactcga
      541 tcgagcgcgt gcgctgggag gaggcgtggc gggtggaaga cggggggctg gtgctcgagc
      601 gcgtgcgcgt gcgcggccac ggggcgggaa tggaaccggc ggagggcgcg gtgctgcgcg
      661 atggcgcctg ggagtggcac ccgcgcagcc gccacgccgt gctgcgcctc acgcgctcgg
      721 cctataccgc cgactacgag tggtgtgcag atggcgagtc ctgcaggccc ttgtccgcgc
      781 tgatcgcctc cgatggcggc gtcaccgagc tgcgtgcctg tccgggaggc gaagcggtgc
      841 gctgagtcgc tcgcgccgcg ctgctcaggc gatgcccgcg gcccccagca gcgcgccgac
      901 caggatcatc cagatcggcg ccagcttcgt ccgcagcgtc accagcgcgg tcagcgtgat
      961 cagcaccagg gcgccgttgc ggtgcgccgg gtcgccgagg aagggcaggg cgagcagcca
     1021 gccggtggcg aataccaggc ccacggtgac gggcgcgagc ccggcggtga acgcgcgcac
     1081 cagcggcgtg tcgcgccggc tcgcgcccca gcggctcacc cccagcgaga gcagcgtcga
     1141 gggcaacagg atgcccacca gcgccgccgc cgccccgggc aggccggcgg cctggaagcc
     1201 gagcaccggc acgaacagca cgttgggccc gggtgccgcc tgggcgagcg cgatcgcggt
     1261 ggtgaagtcg gcgtcgccga tccagccgcg ctcgacgacg aagtagcggt gcatctcggg
     1321 cgcggtcgac atggcgccac cgatcgacag cagcgacagg gtcaggaagt gcaggaagag
     1381 ggcgagcagc tcgcccgccg gcagcgtgtt catcgcattt cctccccgtg cgcggcacgc
     1441 gcgtcgagca ccgtcactcg ccagcgcgcc aggccccagc cgagcaggcc gaggccgatc
     1501 acgatccatg ccagcggcag gcggaacacc gcgatcgcca ccaggctggc gaggccgagt
     1561 gctgcgcaca gccaggggcc gagcaggttg ccgcgcaagg tgccgagcat cttgaaggcc
     1621 atcgccagga tcagccccgc cgccaccgcg cccatgccac gcagcgcgcc ggcgacggcg
     1681 gggtattcgg ccaggcgctc gtagctcgcg gcgagcgcga tcacgatcag gaagggggcg
     1741 agcagcatgc cggtgatggc cgcgagcgcg ccgcgccagc cgaagtagcg gtcgccgatc
     1801 atcagcgaca ggttgaccac gttgggcccg ggcagcatct gcgcgatcga gtacagctcg
     1861 acgaactcgg cgcggctcag ccagccgcgg cggtcgacca gctcgcgctg
//
LOCUS       DAOQNN010000564         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_52754_length_1908_cov_8.335132, whole genome shotgun sequence.
ACCESSION   DAOQNN010000564 DAOQNN010000000
VERSION     DAOQNN010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52754_length_1908_cov_8.335132"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1896)
                     /locus_tag="PKV97_17360"
     CDS             complement(<1..1896)
                     /locus_tag="PKV97_17360"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNB07707.1"
                     /translation="MRDAIAEVEAQQRSPAARSLWWASLAFYDALIHGGLQPDPAVKR
                     LCTQLDAQFRRLLSGTPSVPDRLLRELLYQIARAPVRTEQQRAVRACWQLDAQIPEPG
                     LEVGETPIAPLLDALAARLGEARAHWEAFCAGTAVALPRFEDAVAAMSAPAEALGRPG
                     VRILCAALLDFARWLRRDPLQFVETSGMEVATALLSLEGALDRRPPPAGFGARAEQIA
                     ARLLALQRGEPLSAGAIVAAPADARQAQERAALEQVAKEMLASLAHVEQVLDDFFRNQ
                     ARREPLLQLHQPLQQIIGALSMLGETDALALVRNAAIHIASLAREDSRAGQAEFERLA
                     RDLSALGFYIEAMPRGGARLEELLDPSLGLQGRPATQEDAAAPAAAIAATPVAAPAPT
                     PATEAAAAAPVEMPEQPPPAAAEPPPQDLEAAATQVAAAHPESPENLFGLDFPDLDFS
                     PLEELETPAAMPQAATEPVGTDARAPAAAPELAATVAAESAAAPMAAGAAPVGAEIDA
                     ELLAIFIEEAHEVLAGIAAGLEALRDGTDEPAQLVTIRRGFHTLKGSGRMVGLVQLGD
                     TAWAVEQALNRWLQLDWALTPALFALVAEGHRVFSAWVAQLEHGEAGGGDTAALIAEA
                     RRLHEAAE"
BASE COUNT          226 a          672 c          748 g          262 t
ORIGIN      
        1 ctcggccgct tcgtggagcc gtctcgcctc ggcgatcaga gccgcggtgt cgccaccgcc
       61 ggcctcgcca tgttcgagtt gcgccaccca ggcggagaac acccgatggc cctcggcgac
      121 gagggcgaac aaggcggggg tgagcgccca gtcgagttgc agccagcggt tcagcgcctg
      181 ctccaccgcc cacgcggtgt cgccgagctg gacgagcccg accatgcgcc cgctaccctt
      241 gagggtgtgg aagccgcgcc ggatggtgac cagttgcgcg ggctcgtccg tgccgtcgcg
      301 gagtgcttcg agcccggccg cgatcccggc gagcacctcg tgcgcctcct cgatgaagat
      361 cgccagcagt tcggcgtcga tctccgcgcc caccggcgcc gcccctgcgg ccatcggggc
      421 ggctgcgctc tccgcggcga ccgtggcggc gagttcgggg gcggcggcag gcgcccgcgc
      481 atcggtgccg acgggctctg tcgcggcctg cggcattgcg gcaggtgtct cgagttcttc
      541 gagggggctg aagtcgagat cggggaagtc gaggccgaac aggttttcgg ggctctccgg
      601 gtgggccgct gcgacctgcg tcgccgccgc ctccaggtct tgtggaggcg gctccgcggc
      661 cgcgggcggc ggctgctcgg gcatctcgac gggcgccgct gcggcggctt cggtcgcggg
      721 cgtcggtgcc ggtgccgcaa caggtgtggc ggcgatcgcg gcagcgggcg ccgccgcgtc
      781 ctcctgtgta gccggcctgc cctgaagccc caggctcggg tcgagcagtt cttccaggcg
      841 ggcgccgccg cgcggcatcg cctcgatgta gaagccgagc gcggagaggt cgcgcgcgag
      901 gcgttcgaac tcggcctggc cggcgcggct gtcctcgcgc gcgaggctgg cgatgtggat
      961 cgcggcgttg cgcaccaggg cgagcgcgtc ggtctcgccg agcatgctca gggcaccgat
     1021 gatctgctgc agcggctggt ggagctgcag caggggctcg cgcctggcct ggttgcggaa
     1081 gaagtcgtcc agcacctgtt cgacgtgggc gaggctggcg agcatctcct tcgccacctg
     1141 ctcgagcgcg gcgcgctcct gcgcctggcg ggcatccgcc ggtgctgcga cgatcgcgcc
     1201 cgcggacagg ggctcgccgc gctgcagggc gagcagacgg gcggcgatct gttccgcgcg
     1261 cgcgccgaat ccggcgggcg gcggacgacg gtcgagcgcg ccttcgaggc tcagcagcgc
     1321 ggtggcgacc tccatgcccg aggtctcgac gaactgcagc ggatcgcggc gcagccagcg
     1381 cgcgaagtcg agcagtgcgg cgcacaggat gcgcacgccc gggcggccga gcgcctcggc
     1441 gggggcgctc atcgccgcga ctgcatcctc gaagcggggc agggccaccg cggtgccggc
     1501 gcagaaggct tcccaatgcg cgcgcgcctc gccgaggcgg gcggcgaggg cgtcgagcag
     1561 cggcgcgatc ggtgtctcgc cgacctccag ccccggctcc gggatctgcg cgtcgagctg
     1621 ccagcacgcg cgtaccgcac gctgctgttc agtgcgcacc ggggcgcgcg cgatctggta
     1681 gaggagttcg cgcagcagac ggtccggcac cgagggcgtg ccgctcagca ggcggcggaa
     1741 ctgggcgtcg agctgggtgc acaggcgctt caccgccggg tcgggctgca gcccgccgtg
     1801 gatcagggcg tcgtagaacg cgaggctggc ccaccacaga ctgcgcgcag ccgggctgcg
     1861 ctgctgcgcc tcgacctcgg cgatcgcgtc gcgcatgacg cgcgcgcc
//
LOCUS       DAOQNN010000565         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_52755_length_1908_cov_7.635186, whole genome shotgun sequence.
ACCESSION   DAOQNN010000565 DAOQNN010000000
VERSION     DAOQNN010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52755_length_1908_cov_7.635186"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..>1908
                     /locus_tag="PKV97_17365"
     CDS             106..>1908
                     /locus_tag="PKV97_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB07708.1"
                     /translation="MNHAPAADAATRGRHAGLRLAFPLIALTVLVGGVGAFGYRSLSE
                     EIRRETQRTLAVIAEQKRQHIEGWLAEARVDVRMVFTGHSQLEALFEAWLDGGRRDDA
                     AFARMRALVEELARLRGWQGVALLDADGAPALAVGSPDLSAHAELIADVLRQPRVELV
                     DLHQDARGRTHYGVLAPVGAPSRGVAYLTWEAETALYPMVEAWPVPTRSAETYLVRRE
                     GEGVRFLTPLRHQADAALALERPLATPDLPAARAALGERGILSGGRDYRGVPVLAYAA
                     AVEGTPWLMLAEIDEREAYSGIRTLTWGMGVVMALGLMLVYSGGYLVWRRDREQRERA
                     TLQARQIAEARFRVIFEQAPLGVVLLDPRTRRITEANPRFAAIVGRSVGELVGVDPIA
                     LTHPDDVAESLRQLGRLDAGRIAGYRLNKRYLRPDGTPVWVSLAFAPVQVASEDAPRY
                     LGIVEDISARIEMEERLREASAAAAAANAAKSEFLAHMSHEIRTPMNAVLGLAQVLER
                     EPLAPAQRDMVGRIRGAGASLLAILDDVLDLSKIEAGQLRIEPRPFDLRALLANLDSL
                     MGQAARAKGLALRIEPPALPPGQLRGDGLRIEQIL"
BASE COUNT          231 a          725 c          686 g          266 t
ORIGIN      
        1 ctagacgcct cgaagcgggt tgcagcgcgc ttgccgcagc gcaacaatgc ggacattctt
       61 ctgtctgcgt gcaccggccc ggccgcgatc gcacgcctcc acgccatgaa ccacgctccc
      121 gccgccgatg ccgccacccg cggccgccat gccggcctgc gcctcgcctt tccgctcatc
      181 gccctcaccg tgctggtggg cggcgtgggt gccttcggct accgctcgct gagcgaggag
      241 atccgccgcg agacccagcg cacgctggcg gtcatcgccg agcagaagcg ccagcacatc
      301 gaaggctggc tcgccgaggc gcgcgtggat gtgcggatgg tcttcaccgg ccattcgcag
      361 ctcgaggcct tgttcgaggc ctggctggac ggcggccgcc gcgacgatgc ggcgttcgcg
      421 cgcatgcggg cgctcgtcga ggagctcgcc cgcctgcgcg gctggcaggg cgtcgccctg
      481 ctcgacgccg acggcgcgcc tgcgctcgcg gtcggcagcc ccgacctctc ggcccatgcc
      541 gaactgatcg ccgacgtgct gcggcagccg cgcgtcgagc tcgtcgacct gcaccaggac
      601 gcccgggggc gcacgcatta cggcgtgctc gcgccggtgg gcgcgccgtc gcgcggtgtg
      661 gcctacctga cctgggaggc cgagaccgcg ctgtatccga tggtcgaggc ctggccggtg
      721 cccacgcgca gcgccgagac ttacctggtg cggcgcgagg gcgagggcgt gcgcttcctg
      781 accccgctgc gccatcaggc cgatgcggcg ctcgcgctcg agcgcccgct cgccactccc
      841 gatctgccgg ccgcgcgtgc ggcgctcggc gagcgcggca tcctctccgg cgggcgcgac
      901 taccgcggag tgccggtact ggcctacgcc gccgcggtcg agggcacgcc ctggctgatg
      961 ctcgccgaga tcgacgagcg cgaggcctac tcgggcatcc gcacgctgac ctggggcatg
     1021 ggcgtggtga tggcgctcgg cctcatgctg gtgtactcgg gcggctacct ggtgtggcgg
     1081 cgcgaccgcg agcagcgcga gcgcgccacc ctgcaggccc gccagatcgc cgaggcgcgc
     1141 tttcgggtga tcttcgagca ggcgccgctg ggcgtggtgc tgctcgaccc gcgcacgcgg
     1201 cggatcaccg aggccaaccc gcgcttcgcc gcgatcgtcg gccgcagcgt cggcgagctg
     1261 gtcggcgtcg acccgatcgc gctcactcat ccggatgacg tcgccgagag cctgcgccag
     1321 ctcggccgcc tcgacgccgg gcgcatcgcc ggctaccgcc tcaacaagcg ctacctgcgc
     1381 ccggacggca cgccggtgtg ggtgagcctg gccttcgcgc cggtgcaggt ggcctcggag
     1441 gacgcaccgc gctacctcgg catcgtcgag gacatctccg cgcgcatcga gatggaggag
     1501 cgcctgcgcg aggcctcggc cgcggctgcc gcggccaacg ccgccaagag cgagttcctc
     1561 gcccacatga gccacgagat ccgcacgccg atgaacgcgg tgctcggcct cgcccaggtc
     1621 ctcgagcgcg agccgctcgc gcctgcgcag cgcgacatgg tcgggcgcat ccgcggcgcc
     1681 ggcgcttcgc tgctggcgat cctcgacgac gtgctcgacc tctccaagat cgaggccggc
     1741 cagctgcgca tcgagccgcg gcccttcgac ctgcgcgcgc tgctcgccaa tctcgacagc
     1801 ctgatgggtc aggccgcgcg tgcgaagggg ctggcgctgc gcatcgagcc gccggcgctg
     1861 ccgcccgggc agctgcgcgg cgatgggctg cgcatcgagc agatcctc
//
LOCUS       DAOQNN010000566         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_53000_length_1903_cov_7.060065, whole genome shotgun sequence.
ACCESSION   DAOQNN010000566 DAOQNN010000000
VERSION     DAOQNN010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53000_length_1903_cov_7.060065"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PKV97_17370"
     CDS             complement(<1..994)
                     /locus_tag="PKV97_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNB07709.1"
                     /translation="MTTQTLSMFDPALLRPAIADAFVKLDPRVQWRNPVMFVVYVGSL
                     FTTALWAQALAGHGEAPAWFIGATALWLWFTVLFANFAEALAEGRSKAQAASLRSAKQ
                     TVTAKKLREPRRDAPVEPVPGSTLRRDDVVLVEAGDMIPGDGEVIAGVASVNESAITG
                     ESAPVIREAGGDFSAVTGGTTVLSDWIVVRITVNPGETFVDRMIALVEGAKRQKTPNE
                     IALTILLVALTLIFLLATVTLLPYSLFAVVAAGAGQPVTLTALVALLVCLIPTTIGAL
                     LSAIGIAGMDRLVRFNVLAMSGRAVEAAGDVDTLLLDKTGTITVGNRQATAFRPI"
     gene            complement(1007..>1903)
                     /gene="kdpA"
                     /locus_tag="PKV97_17375"
     CDS             complement(1007..>1903)
                     /gene="kdpA"
                     /locus_tag="PKV97_17375"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNB07710.1"
                     /translation="RAVGDSRQGFAVLAAMTVIFVGATIGAMHFEQQGNPLLTTLGAD
                     QTASALQSGGNLEGKETRYGIGASALWAAATTSASNGSVNSMHDSYTPLGGLVPMLMM
                     QLGEVVFGGVGSGLYGMLVFALMAVFIAGLMIGRTPEYLGKKIESFDMKMVAIGILVT
                     PLLVLLGTALAVVTDAGRATIFNPGAHGFAEVLYAFSSAANNNGSAFAGLGANNPFYN
                     GALGVAMWFGRFAVIVPVLAIAGSLAAKKTLAQGPGTLPTHGPLFVGLLVGTVLLIGA
                     LTYVPALALGPAVEHLMLWAGW"
BASE COUNT          323 a          680 c          642 g          258 t
ORIGIN      
        1 ggatcggccg gaacgcggtc gcctgccgat ttccgacagt gatcgtgccg gtcttgtcga
       61 gcagcagcgt gtcgacgtcg ccggcggcct cgaccgcccg ccccgacatg gcgagcacgt
      121 tgaagcggac gagacgatcc atgccggcga tgccgatcgc cgacagcagc gcgccgatgg
      181 tggtcgggat caggcacacc agcagcgcca ccagcgcggt gagcgtcacc ggctgacccg
      241 cgccggcggc gaccaccgcg aacagcgagt agggcagcag cgtgaccgtc gcgagcagga
      301 agatcagcgt cagcgccacc agcaggatgg tgagcgcgat ctcgttcggc gtcttctggc
      361 gcttggcgcc ctcgaccagc gcgatcatgc ggtcgacgaa ggtctcgccg ggattcacgg
      421 tgatgcgcac cacgatccag tccgacagca ccgtggtgcc gccggtgacg gccgagaaat
      481 cgccgccggc ctcgcggatc accggcgccg actcgccggt gatggcgctc tcattgaccg
      541 aggccacgcc cgcgatcacc tcgccgtcac ccggaatcat gtcgccggcc tccaccagca
      601 ccacgtcatc gcggcgcagc gtgctgccgg gcaccggctc taccggcgca tcacggcgcg
      661 gctcgcgcag cttcttcgcc gtcaccgtct gcttggcgct gcgcagggag gccgcctgcg
      721 ccttgctgcg gccctcggcg agcgcctcgg cgaagttcgc gaacagcacc gtgaaccaca
      781 gccacagcgc cgtggcgccg atgaaccagg ccggggcctc gccgtgtccg gcgagcgcct
      841 gtgcccacag cgcggtggtg aacaggctgc ccacatagac cacgaacatc accgggttgc
      901 gccactgcac gcgcgggtcg agcttgacga aggcgtccgc gatggccggg cgcagcagcg
      961 cgggatcgaa catcgaaagg gtctgggtcg tcatggggtg atctccttac cagccggccc
     1021 acaacatcag atgctcgacc gccggcccga gcgccagcgc cggcacatag gtcagcgcac
     1081 cgatcagcag caccgtgccc accaggagcc ccacgaacag cggcccgtgg gtgggcagcg
     1141 tgcccgggcc ctgggcgagc gttttcttgg cggcgagcga acccgcgatc gccagcaccg
     1201 gcacgatgac cgcgaagcgc ccgaaccaca tcgccacacc cagggcgccg ttgtagaacg
     1261 ggttgttggc gccgaggccc gcaaaggcgc tgccgttgtt gttggcggcc gacgagaagg
     1321 cgtacagcac ttccgcaaaa ccgtgcgcgc cggggttgaa gatcgtcgcc cggccggcat
     1381 cggtcaccac cgcgagcgcg gtgccgagca gcaccagcaa tggggtgacc aggatgccga
     1441 tcgccaccat cttcatgtcg aaggactcga tcttcttgcc gaggtattcc ggcgtgcgcc
     1501 cgatcatgag cccggcgatg aacaccgcca tgagtgcgaa caccagcatg ccgtagagcc
     1561 ccgagccgac gccgccgaac accacctcgc cgagctgcat catgagcatc ggcacgagac
     1621 ccccgagcgg ggtgtaggag tcgtgcatcg agttgaccga gccgttcgag gcggaagtcg
     1681 tggccgccgc ccacagcgcc gaggcgccga tgccgtagcg ggtctccttg ccctccaggt
     1741 tgccgcccga ttgcagcgcg ctcgcggtct ggtcggcacc gagcgtcgtg agcagcggat
     1801 tgccctgctg ctcgaagtgc atggccccga tcgtggcgcc gacgaagatc accgtcatcg
     1861 ccgccagcac cgcgaagccc tggcggctgt cgccaacggc gcg
//
LOCUS       DAOQNN010000567         1895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_53394_length_1895_cov_13.332609, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000567 DAOQNN010000000
VERSION     DAOQNN010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53394_length_1895_cov_13.332609"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1895)
                     /locus_tag="PKV97_17380"
     CDS             complement(<1..>1895)
                     /locus_tag="PKV97_17380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNB07711.1"
                     /translation="IETPIISNFKEGLTVLEYFNSTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDAIITEEDCGTKNGLTAKAVVEGGEVIAALSDRILGRAAAEDVKDPLSGKI
                     IVKAAQLIDEPLVDEIERAGIDVVKIRSVLTCEAKTGVCGKCYGRDLARGTPVNIGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAAQRGAEQSSIEATIDSIVDVRNRNVVLNSQGV
                     PVVMGRNCEIVLTDEQGRERARHRVPYGARLLADHGQKVTKGAKLAEWDPYTLPIITE
                     REGIVNYIDLVEGVSMREVMDETTGISNRVVIDWKQQPRGNDLKPRITLRDDKGNVIM
                     LPNGLEARYFMSVDAILSVENGAHVKAGDVLARIPREGTKTRDITGGLPRVAELFEAR
                     KPKDFAIISEIAGRVEFGKDYKTKRRIVVVPEDASEESREYLIPKGKHISVQEGDYVQ
                     KGDQLLDGNPVPHDILNVMGVEALATYLINEIQEVYRLQGVKINDKHIEVIVRQMLQK
                     IEISDPGDTTFLAGEQVDRSEYEEENAKALKEGGRLAKGSPVLLGITKASLQTRSFIS
                     AASFQETTRVLTEAAVSGRVDSLIGLKENVIVGRLIPAGTGSVMNRLKHEAAERDKEL
                     AAQKPAA"
BASE COUNT          264 a          641 c          623 g          367 t
ORIGIN      
        1 ggccgccggc ttctgcgccg ccagctcctt gtcgcgctcg gccgcctcgt gcttcaggcg
       61 gttcatcacc gagccggtgc cggccgggat caggcggccg acgatcacgt tctccttgag
      121 gccgatcagg ctgtcgaccc ggccggaaac cgcggcctcg gtcagcacgc gcgtcgtctc
      181 ctggaacgag gcggcggaga tgaaggaacg ggtctgcaag gacgccttgg tgatgccgag
      241 cagcaccggg gagcccttag ccaggcggcc gccttccttc aacgccttcg cattctcctc
      301 ctcgtattcc gagcggtcga cctgctcgcc cgccaggaac gtggtgtcgc ccggatcgga
      361 gatctcgatc ttctgcagca tctggcggac gatcacctcg atgtgcttgt cgttgatctt
      421 cacgccctgc aggcgataga cctcctggat ctcgttgatg agataggtgg ccagcgcctc
      481 gacgcccatg acgttgagga tgtcatgcgg caccgggttg ccgtccagca actggtcgcc
      541 cttctggacg tagtcgcctt cctgcaccga gatatgcttg cccttcggga tcaggtattc
      601 gcgcgattcc tccgaggcat cctccggcac cacgacgatg cggcgcttgg tcttgtagtc
      661 cttgccgaac tcgacgcggc cggcgatctc gctgatgatg gcgaaatcct tcggcttgcg
      721 cgcctcgaac agctcggcca cgcgcggcag accgccggtg atgtcgcggg tcttggtgcc
      781 ctcgcgcggg atgcgcgcca gcacgtcgcc cgccttcacg tgcgcgccgt tctcgaccga
      841 gagaatggcg tccaccgaca tgaagtagcg cgcctcgagg ccgttgggca gcatgatcac
      901 gttgcccttg tcatcgcgca gcgtgatgcg cggcttcaag tcgttgccgc gcggctgctg
      961 cttccagtcg atgaccacgc ggttggagat gccggtggtc tcgtccatca cttcgcgcat
     1021 cgacacgcct tcgaccaggt cgatgtagtt cacgatgcct tcgcgctcgg tgatgatcgg
     1081 cagggtgtac ggatcccact ccgccagctt ggcgcccttc gtcaccttct ggccgtgatc
     1141 ggccagcagg cgggcgccat acggaacgcg gtgacgcgcc cgctcgcggc cctgctcgtc
     1201 ggtcagcacg atctcgcaat tgcggcccat gaccacgggc acgccctggc tgttgagcac
     1261 cacgttgcgg ttgcgcacat cgacgatgga atcgatggtc gcctcgatcg agctctgctc
     1321 ggcgccgcgc tgggcggcgc cgccgatgtg gaacgtgcgc atggtgagct gggtgcccgg
     1381 ctcgccgatc gactgcgccg cgatcacgcc gatcgcctcg ccgatgttga ccggggtgcc
     1441 gcgcgccagg tcgcggccgt agcacttgcc gcagacgccg gtcttggcct cgcaggtcag
     1501 caccgagcgg atcttcacca cgtcgatgcc ggcgcgctcg atctcgtcga ccagcggctc
     1561 gtcgatgagc tgcgccgcct tgacgatgat cttgccggag agcgggtcct tcacgtcctc
     1621 ggccgcggcg cggcccagga tgcggtcgga gagcgcggcg atgacttcgc cgccctcgac
     1681 caccgccttg gcggtgaggc cgttcttggt gccgcaatcc tcctcggtga tgatggcgtc
     1741 ctgcgccacg tccaccaggc ggcgggtcag gtagcccgag ttcgccgtct tcaaggcggt
     1801 gtcggccagg cccttgcggg cgccgtgggt cgagttgaaa tactcgagca cggtgaggcc
     1861 ttccttgaag ttcgagatga tcggcgtctc gatga
//
LOCUS       DAOQNN010000568         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_53647_length_1890_cov_11.798365, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000568 DAOQNN010000000
VERSION     DAOQNN010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53647_length_1890_cov_11.798365"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..392
                     /gene="rplN"
                     /locus_tag="PKV97_17385"
     CDS             24..392
                     /gene="rplN"
                     /locus_tag="PKV97_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNB07712.1"
                     /translation="MIQMQSRLDVADNTGAKSVMCIKVLGGSKRRYAGIGDVIKVSIK
                     EAAPRGRVKKGEVYSAVVVRTAKGVRRQDGSLIKFDGNAAVLLNNKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            413..730
                     /gene="rplX"
                     /locus_tag="PKV97_17390"
     CDS             413..730
                     /gene="rplX"
                     /locus_tag="PKV97_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNB07713.1"
                     /translation="MNKIRKGDEVIVLTGRDKGKRGTVSLRVDEDHVVVDGINVVKKH
                     VRPNPMKGTTGGIVDKTMPIHQSNVAIYNPASGKADRVGIKLLADGKKVRVFKSSGEE
                     IKA"
     gene            745..1302
                     /gene="rplE"
                     /locus_tag="PKV97_17395"
     CDS             745..1302
                     /gene="rplE"
                     /locus_tag="PKV97_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNB07714.1"
                     /translation="MAQQQAQARLQAFYREKVVPDLMAKFGYKSVMEVPRFEKITLNM
                     GVSEAVSDKKVMEHAVGDLTKIAGQKPVVTKSRKPIAGFKIRENVPIGCMVTLRGVRM
                     YEFLDRFVTIALPRVRDFRGISGRSFDGRGNYNIGVKEQIIFPEIEYDKVDALRGLNI
                     SITTSAKTDDECKALLSAFRFPFKN"
     gene            1311..1616
                     /gene="rpsN"
                     /locus_tag="PKV97_17400"
     CDS             1311..1616
                     /gene="rpsN"
                     /locus_tag="PKV97_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNB07715.1"
                     /translation="MAKLSLKQREEKREKLVAKYAKKYAELKAIIDDQSKSEDERYLA
                     RLALQKLPRNANPTRLRARCEITGRPRGTFRKFGLGRTKIRELAFRGDIPGVVKASW"
     gene            1653..>1890
                     /gene="rpsH"
                     /locus_tag="PKV97_17405"
     CDS             1653..>1890
                     /gene="rpsH"
                     /locus_tag="PKV97_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNB07716.1"
                     /translation="MSMSDPIADMLTRIRNAQQVEKSSVAMPSSKLKAAIAQVLKDEG
                     YIDGFAVKSDGGKAQLEIALKYYAGRPVIERIERV"
BASE COUNT          413 a          542 c          616 g          319 t
ORIGIN      
        1 gcgggataag ttgggaacaa gacatgatcc aaatgcaatc gcgactggac gtcgcggaca
       61 acaccggtgc gaagtccgtc atgtgcatca aggtgctggg cggctcgaag cgtcgttacg
      121 ccggtatcgg tgacgtgatc aaggtcagca tcaaggaagc tgcgccgcgt ggtcgcgtca
      181 agaagggcga ggtctacagt gccgtggtcg tgcgcaccgc caagggtgtg cgccgccagg
      241 atggctcgct gatcaagttc gatggcaatg ccgcggtgtt gctgaacaac aagctcgagc
      301 cgatcggcac gcgcatcttc ggcccggtga cccgcgaact gcggaccgag cgtttcatga
      361 agatcgtgtc gctggctccc gaggtgctct gagcgaacgc gaggaacagg ccatgaacaa
      421 gattcgcaag ggcgacgaag tcatcgtgtt gacgggtcgc gacaagggta agcgtggcac
      481 ggtgtcgctg cgcgtggacg aagatcacgt ggtggtcgac ggcatcaatg tcgtcaagaa
      541 gcatgtgcgt ccgaatccca tgaagggcac gaccggtggc atcgtcgaca agaccatgcc
      601 gatccaccaa tccaatgtgg cgatctacaa cccggcgtcc ggcaaggccg accgtgtggg
      661 gatcaagctg ctggctgacg gcaagaaagt gcgcgtcttc aagtccagcg gcgaagagat
      721 caaggcttga ggcggggtag cgaaatggct caacaacaag cacaagcccg cctgcaggcg
      781 ttctaccgcg agaaggtggt tccggatctg atggccaagt tcggctacaa gtccgtgatg
      841 gaggtgccgc gcttcgagaa gatcaccctg aacatgggtg tttcggaagc ggtttccgac
      901 aagaaggtca tggagcacgc cgtgggcgac ctgaccaaga tcgctggcca gaagccggtg
      961 gtgaccaagt cgcgcaagcc gatcgccggg ttcaagatcc gcgagaacgt gcccatcggc
     1021 tgcatggtga ccctgcgcgg cgtgcgcatg tacgaattcc tggaccgttt tgtcaccatc
     1081 gcgctgccgc gcgtgcggga cttccgcggt atctcgggtc gttccttcga tggccgtggc
     1141 aactacaaca tcggcgtcaa ggaacagatc atcttcccgg aaatcgagta cgacaaggtg
     1201 gatgcgctgc gtggtctgaa catcagcatc acgacgtcgg ccaagaccga cgacgagtgc
     1261 aaggcgctgc tgtcggcctt ccgcttcccg ttcaagaact gaggtccgac gtggcaaaac
     1321 tctccctcaa gcagcgcgag gaaaagcgcg agaagctggt cgcgaagtac gcgaagaagt
     1381 acgccgagct gaaggccatc atcgacgatc agtccaagtc ggaagacgag cgttatctgg
     1441 cccgcctggc actgcagaag ctgccgcgca atgccaaccc gacccgcctg cgcgcccgtt
     1501 gcgaaatcac cggtcgtccg cgtggcacct tccgcaagtt cggtctgggg cgcaccaaga
     1561 tccgtgaact ggcgttccgt ggcgacatcc ccggtgtcgt caaggccagc tggtaagccg
     1621 gcgtcctcag ggcacgaaca ggagtaatcc ccatgagcat gagtgatccc atcgcggaca
     1681 tgctgacccg cattcgcaat gcgcaacagg tcgagaagag cagcgtggcg atgccttcgt
     1741 ccaagctgaa ggctgcgatc gcgcaggtgc tgaaggacga gggctacatc gacggcttcg
     1801 cggtcaagag cgacggcggc aaggcccagc tcgaaatcgc gctgaagtat tacgccggcc
     1861 gtccggtgat cgagcgcatc gagcgcgtca
//
LOCUS       DAOQNN010000569         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_54032_length_1882_cov_6.741653, whole genome shotgun sequence.
ACCESSION   DAOQNN010000569 DAOQNN010000000
VERSION     DAOQNN010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54032_length_1882_cov_6.741653"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKV97_17410"
     CDS             <1..229
                     /locus_tag="PKV97_17410"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNB07717.1"
                     /translation="VGRVLRYRGRLRPLMKGEDVKHVQRAVGLTGDAVDGKYGPGTRD
                     AVRAAQRRLHIPDDGVVGRQTVEALGGRWLG"
     gene            226..678
                     /locus_tag="PKV97_17415"
     CDS             226..678
                     /locus_tag="PKV97_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07718.1"
                     /translation="MSIWQQTLGILGAGGFGGVATYVLTLRERRAVLAAQASKVEDEG
                     TALVVRASASLLEQAGLAVPALSAAVARLEAELKAERERGERLEAQVAADREETAEVH
                     RWIGDAWHWMQRAYREITERGGSIDAPPQPPTRAAVFRRPQPPATFTQ"
     gene            690..914
                     /locus_tag="PKV97_17420"
     CDS             690..914
                     /locus_tag="PKV97_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07719.1"
                     /translation="MLDKLTPLLRHLALLILAAVVTAFVSWIQSDLPGVLAETALAPF
                     AGAIVTALVAVLTPLTRQYGVGAPDGGDDQ"
     gene            911..1240
                     /locus_tag="PKV97_17425"
     CDS             911..1240
                     /locus_tag="PKV97_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07720.1"
                     /translation="MTQPQDQADCPLRDSHGNPFRYCAACSWVEPPASGTQPLTDEER
                     ARFTYHPATPMTGPRHDAVRAAAAAYAAALAAVVPAGRHRSLALTAAQESMMWANAGI
                     ACDTPQG"
     gene            complement(1356..1760)
                     /locus_tag="PKV97_17430"
     CDS             complement(1356..1760)
                     /locus_tag="PKV97_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07721.1"
                     /translation="MSDYAPRHSLPAAEVFPAPAVDSPPVQPRRRDRRPVYAALAVLI
                     VALAATWWFVGPNERNCERQIDSYLVKMLASKTPMSEQPDPNSSQFWQCWGLSDGAKR
                     RAASGAFSKHIGEMMGRAFSEAFTDSFGATTP"
BASE COUNT          236 a          698 c          707 g          241 t
ORIGIN      
        1 ggtcggacgg gtcctgcgct accgcggccg gctccgcccg ctgatgaagg gcgaggacgt
       61 caagcacgtg cagcgcgccg tcggcctcac cggcgacgcc gtcgacggga agtacggccc
      121 gggcacccgg gacgccgtcc gggccgcgca gcgccgcctg cacatcccgg acgacggcgt
      181 cgtcggccgc cagaccgtcg aggccctcgg cggccggtgg ctcggatgag catctggcag
      241 cagaccctcg gcatcctcgg cgccggcggg ttcggcggcg tcgccaccta cgtcctgacg
      301 ctgcgggagc gccgcgccgt cctcgccgcg caggcgtcga aggtcgagga cgagggcacc
      361 gccctcgtcg tccgggcgtc cgcgtcgctc ctggagcagg ccggcctggc cgtcccggcg
      421 ctgtcggccg cggtagcccg gctcgaggcg gagctgaagg ccgagcggga gcgcggcgag
      481 cggctcgagg cgcaggtcgc cgcggaccgc gaggagacgg ccgaggtgca ccggtggatc
      541 ggtgacgcct ggcactggat gcagcgcgcc taccgcgaga tcaccgagcg gggcgggtcg
      601 atcgacgcgc cgccgcagcc gcccaccagg gcggccgtct tccgtcgtcc gcagccgccg
      661 gcgacgttca cgcagtgaag ggatccgtca tgctcgacaa gctcacgccg ctgttgcggc
      721 acctcgcgct gctcatcctg gcggccgtcg tcacggcgtt cgtgtcgtgg atccagtccg
      781 acctgccggg cgtgctcgcc gagacggccc tcgcgccgtt cgccggcgcg atcgtcacgg
      841 ccctcgtcgc ggtgctgacg ccgctgacgc ggcagtacgg ggtcggcgca ccggacggcg
      901 gcgacgacca gtgacacagc cgcaggacca ggccgactgc ccactgaggg actcgcacgg
      961 caacccgttc cgctactgcg cggcctgctc atgggtggag ccgccggcgt ccggaacgca
     1021 gccgctcacc gacgaggagc gggcccggtt cacctaccac ccggcgacgc cgatgaccgg
     1081 gccgcgacac gacgccgtcc gggcggccgc ggccgcctac gctgcggcgc ttgccgccgt
     1141 cgtcccggcc ggccgtcacc ggtcgctcgc gctcaccgcc gcgcaggagt cgatgatgtg
     1201 ggcgaacgct gggatcgcct gcgacacccc gcagggttga cggttcgtcc cccgggccgg
     1261 gcagggaccg ggggtgcgcg cggcgggcgg gcctgcgcgc agagagccgg gccggacctt
     1321 cacgggtccg gcccggctct tcgtcgtttt cggccctacg gcgtcgtcgc gccgaacgag
     1381 tccgtgaagg cctcgctgaa cgcgcggccc atcatctccc cgatgtgctt cgagaacgcg
     1441 cccgacgccg cgcgccgctt cgccccgtcg ctcagacccc agcactgcca gaactggctg
     1501 gaattcgggt ccggctgctc actcatcggc gtcttcgacg cgagcatctt caccaggtac
     1561 gagtcgatct gccgctcaca gttccgctcg ttcgggccga cgaaccacca ggttgccgcg
     1621 agcgcaacga tgaggacggc gagggcggcg tacacggggc gccggtcccg gcggcgcggc
     1681 tggacgggcg gcgagtcgac ggcgggcgcc gggaagacct cggcggccgg caggctgtgg
     1741 cgcggggcgt agtcgctcat ggccgcaggg tacggcccgt gatcgatcac aggtggtcgg
     1801 tcgatcgcac gagggttccg cgcgcgcatc ggcgcaggtc gccgtcgcgt cgcgccgacg
     1861 cgcgtagacg agcgtttccg cg
//
LOCUS       DAOQNN010000570         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_54341_length_1876_cov_5.528830, whole genome shotgun sequence.
ACCESSION   DAOQNN010000570 DAOQNN010000000
VERSION     DAOQNN010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54341_length_1876_cov_5.528830"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1347
                     /locus_tag="PKV97_17435"
     CDS             55..1347
                     /locus_tag="PKV97_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNB07722.1"
                     /translation="MTRDTSQWRFETKSVHAGYSPEPTTHAVAVPLYQTVAYSFDSAQ
                     HGADLFDLKVAGNIYTRIMNPTQAVLEERIAALEGGIASLALASGQAAITYAIQTITE
                     AGDNIVASSALYGGTYNLFAHTLPQYGIQARFADYRDPKSFEPLIDARTKAVFVESLG
                     NPQGNITDLAAIAAIAHKHGVPLIVDNTVPSPYLCRPFEHGADIVVHSLTKYLGGHGT
                     SIGGAIVDSGKFPWAQHKAKFKRLNEPDVSYHGVVYTEALGPAAYIGRARVVPLRNMG
                     AAISPFNAWQILQGVETLALRMDRICENALAVARHLKAHPKVAWVNYAGLEDHPDHAL
                     VKKQLGGRASGLLTFGVKSGRAGGERFLDALQLFTRLVNIGDTKSLATHPASTTHRQL
                     SPEELKKAGVSEETVRLCVGIEHIDDLRADLDAALAKA"
     gene            1450..>1876
                     /locus_tag="PKV97_17440"
     CDS             1450..>1876
                     /locus_tag="PKV97_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNB07723.1"
                     /translation="MTFSLSRRLLAALAAITAALALSGCGYNDFQRLDEQTKSAWSEV
                     LNQYQRRADLIPNIVATVKGEANFEQETLTKVVEARAKATSIQATPELVNDPAAFQKF
                     QAAQGELSSALSRLLVVSENYPNLKANQGFQDLRVQLEGT"
BASE COUNT          331 a          699 c          606 g          240 t
ORIGIN      
        1 gaggctcggg tgggcataga ctgcccagcc ctgccgaacc ccaaggagac ccgcatgacc
       61 cgcgacacca gccaatggcg cttcgagacc aagtcggtcc acgccggcta ttcgcccgag
      121 cccaccacgc acgccgtggc cgtgccgctg taccagacgg tggcctactc gttcgacagc
      181 gcgcagcacg gcgccgacct gttcgacctc aaggtggcgg gcaacatcta cacccgcatc
      241 atgaacccga cgcaggcggt gctcgaggag cgcatcgccg cgctcgaggg cggcatcgcc
      301 tcgctcgcgc tcgcctccgg ccaggccgcg atcacctacg cgatccagac catcaccgag
      361 gccggcgaca acatcgtcgc cagcagcgcg ctgtacggcg gcacctacaa cctgttcgcg
      421 cacacgctgc cgcagtacgg catccaggcg cgcttcgccg actaccgcga cccgaagagc
      481 ttcgagccgc tgatcgacgc gcgcaccaag gccgtgttcg tcgagtcgct cggcaacccg
      541 cagggcaaca tcaccgacct ggccgccatc gccgcgatcg cccacaagca cggcgtgccg
      601 ctgatcgtcg acaacacggt gcccagcccg tatctctgcc gcccgttcga gcacggcgcc
      661 gacatcgtcg tgcactcgct gaccaagtac ctcggcggcc acggcaccag catcggcggc
      721 gcgatcgtcg actccggcaa gttcccatgg gcgcagcaca aggcgaagtt caagcgcctg
      781 aacgagcccg acgtcagcta ccacggcgtg gtctacaccg aggcgctcgg cccggcggcc
      841 tacatcggcc gcgcgcgcgt ggtgccgctg cgcaacatgg gcgcggcgat ctcgccgttc
      901 aacgcctggc agatcctgca gggcgtggag acgctggcgc tgcgcatgga ccgcatctgc
      961 gagaacgcgc tggcggtggc gcgccacctg aaggcgcacc cgaaggtggc ctgggtcaac
     1021 tacgccggcc tggaagacca ccccgaccac gcgctggtga agaagcagct cggcggccgc
     1081 gcctccggcc tgctgacctt cggcgtcaag agcggccgcg ccggcggcga gcgcttcctc
     1141 gacgcgctgc agctgttcac gcggctggtg aacatcggcg acaccaagtc gctggccacg
     1201 cacccggcct cgaccacgca ccgccagctc tcgccggaag agctgaagaa ggcgggtgtc
     1261 agcgaggaga cggtacggct gtgcgtgggc atcgagcaca ttgacgacct gcgcgccgac
     1321 ctggacgccg cgctggcgaa ggcctgaggc ccctcggagg gcgcggggct gattgaaatc
     1381 agccccgccg gtcccacatc ggggagcgcg ggggtacgat cctcgcgcca ccctggggag
     1441 aaaccgaaca tgaccttctc gctctcgcgc cgcctgctcg ccgccctggc cgcgatcacc
     1501 gccgcgctcg ccctcagcgg ctgcggctac aacgacttcc agcgcctcga cgagcagacc
     1561 aagtcggcct ggtcggaggt gctgaaccag taccagcgcc gcgccgacct gatcccgaac
     1621 atcgtggcca ccgtcaaggg cgaggccaac ttcgagcagg agacgctgac caaggtggtg
     1681 gaggcgcgcg ccaaggccac ctcgatccag gccacgccgg aactcgtcaa cgatccggcc
     1741 gcgttccaga aattccaggc ggcgcagggc gagctctcga gcgcgctgtc gcgcctgctg
     1801 gtcgtcagcg agaactaccc gaacctgaag gccaaccagg gcttccagga cctgcgcgtg
     1861 cagctcgagg gcaccg
//
LOCUS       DAOQNN010000571         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_54558_length_1872_cov_5.329114, whole genome shotgun sequence.
ACCESSION   DAOQNN010000571 DAOQNN010000000
VERSION     DAOQNN010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54558_length_1872_cov_5.329114"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..>1872)
                     /locus_tag="PKV97_17445"
     CDS             complement(343..>1872)
                     /locus_tag="PKV97_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNB07724.1"
                     /translation="CTNPAQSMPEQALVHEALAACEFVVLQEAFAGTETAAFADLVLP
                     AATWGEKEGTVTNSERRISRVRAAVPAPGEARADWRIVCDFARALAPRIGKPQAAAMF
                     AYASAQAIFAEHVASTAGRDLDITGLDYSLLEGEGPQQWPFPAGAAVDGADAAARARL
                     YADGRFATPNGRARFVVPARTLTAEATGVRYPLALTSGRLRDHWHGLSRSGKVARLYG
                     HADEARIELHPQELARRGLVDGDLVKVSSRHGELVLRVAAASALRHGMAFVGMHWGRR
                     ALNSAGINLLFGGACDPLSKQPELKHAAVRIEPVELPNRMLVVRAERPGRTAAEEAAL
                     LSPWLERFAYASLALAGRDTPAVVMRVAHDRPIPAGWLAELDALLGLEGDGVLSYADP
                     ARGISKRALIEDGRLVGLRLTGETAAAGWLSEAVVERRDAAALRPWLLAPLATPPTGD
                     AGRGRVVCNCLNVSEADITAAIAAGAGVRDFDALQARLRCGTSCGSCVPEIRRMLAEG
                     S"
BASE COUNT          263 a          707 c          646 g          256 t
ORIGIN      
        1 gatcgcgact tgcgtcgctc ccacagaacc gccaacgccc ggtgtgggac gtaggcgcga
       61 cgcgagtcgc gatccagcgg tcgatcaatc gcggcgcgga tcccgcgcgc tgcgatcgcg
      121 acttgcgtcg ctcccacaga accgccaacg cccggtgtgg gacgtaggag cgacgcaagt
      181 cgcgatgcat tcggccaaaa tctagaaatt cgccttatag tcgaagcatt attttttgtt
      241 gcactgcaca aaaaatttct ctacgattgc acctgtctcc tccaccctcc tcctttggtg
      301 tggatttgcc cgggcctcgc aagacgcccg ggctttttct tttcagctcc cctcggcgag
      361 catgcggcgg atctcgggca cgcacgaacc gcaggacgtg ccgcagcgca gcctggcctg
      421 cagcgcgtcg aagtcgcgca cgccggcgcc cgcggcgatc gccgccgtga tgtcggcctc
      481 ggacacgttc aggcagttgc acaccacccg cccgcgcccc gcatctcccg tcggcggggt
      541 cgccagcggt gccagcagcc acggacgcag cgccgccgcg tcgcggcgct cgaccaccgc
      601 ctcgctcagc cagcccgcgg ctgcggtctc gccggtcagg cgcaggccga ccaggcggcc
      661 gtcctcgatc agcgcacgct tgctgatccc gcgcgcgggg tcggcatacg acagcacccc
      721 gtcgccctcg aggccgagca aggcgtcgag ctcggcgagc cagccggccg ggatcggacg
      781 gtcgtgcgcg acccgcatca ccaccgcggg cgtgtcgcgg ccggcgagcg cgagcgaggc
      841 gtaggcgaag cgctccagcc agggcgacag cagcgcagcc tcctcggcgg cggtacgccc
      901 ggggcgctcg gcacgcacca ccagcatgcg gttcggcagc tcgaccggct cgatgcgcac
      961 cgccgcgtgc ttgagctccg gctgcttcga cagcggatcg caggcgccgc cgaagagcag
     1021 gttgatcccg gccgagttca gcgcccgccg cccccagtgc atcccgacga aggccatgcc
     1081 gtgccgtagc gcgctcgccg cggccacgcg cagcaccagc tcgccgtgcc ggctcgacac
     1141 cttgaccagg tcgccgtcga cgaggccgcg ccgcgccagc tcctgcgggt gcagctcgat
     1201 gcgtgcctcg tccgcatggc cgtacaggcg cgccaccttg ccgctgcggc tcaggccgtg
     1261 ccaatggtcg cgcaggcggc cgctggtgag ggcgagcgga tagcgcacgc cggtcgcctc
     1321 ggcggtgagc gtgcgtgccg gcacgacgaa gcgcgcccgc ccgttcggcg tggcgaagcg
     1381 cccatcggcg tagagacgcg cccgcgccgc ggcatcggcg ccatccaccg ccgcccccgc
     1441 cggaaacggc cactgctgcg ggccttcacc ttccagcagg gaatagtcca gcccggtgat
     1501 gtcgaggtcg cgcccggcgg tgctggcgac gtgctcggcg aagatcgcct gcgccgacgc
     1561 gtaggcgaac atcgccgccg cctgcggctt gccgatgcgc ggtgcgagcg cgcgtgcgaa
     1621 gtcgcacacg atgcgccagt cggcacgcgc ctcgcccggc gccggcacgg ccgcgcgcac
     1681 gcggctgatg cggcgctcgg agttggtcac cgtgccctcc ttctcccccc aggtggcggc
     1741 cggcagcacg aggtcggcga aggccgcggt ctcggtgccg gcgaaggcct cctgcagcac
     1801 gacgaactcg caggcggcga gcgcctcgtg caccagggcc tgctcgggca tggactgcgc
     1861 cgggttggtg ca
//
LOCUS       DAOQNN010000572         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_55431_length_1856_cov_14.976124, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000572 DAOQNN010000000
VERSION     DAOQNN010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55431_length_1856_cov_14.976124"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PKV97_17450"
     CDS             complement(<1..373)
                     /locus_tag="PKV97_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931192.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HNB07725.1"
                     /translation="MAYALQQLINGLTLGAMYGLIAIGYTMVYGIIGMINFAHGDIFM
                     VSAFIAVTAFTILAAADVTSIPLALTFVLIVSIFFTSAYGWAVERTAYRPLRGSTRLA
                     PLISAIGMSIFLQNSVQLTQGA"
     gene            complement(583..1677)
                     /locus_tag="PKV97_17455"
     CDS             complement(583..1677)
                     /locus_tag="PKV97_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNB07726.1"
                     /translation="MKKTLLATAVLALGGFSNAALADLAVAIAGPMTGQYASAGDQIR
                     KGAEMAIADINARGGVLGQKLKLEVGDDACDPKQAVSVANTMVNKGIVFMHGHWCSSS
                     TIPASEVYVEADIPMATVSTNPQVTERGLKNIFRIMGRDDQQGLVAGGYIADHFKGKK
                     VAVVDDKSAYGKGLADEMAKAMEGKGLKPALRESITAGEKDYSGIVTKLKQAGVEVMA
                     YGGYHTEVALILRQAQAAGLQLTVMGGDTMTNSELVTAAGPAADNVMFTFSPDARKNP
                     DAAPVVEKFRAAKTEPEGYVLYAYAAMQLFEQAATTAKSTKYADLEKAMRGGSFKTVI
                     GDLAFDAKGDQKAPGFVVYRWQGGKYDYAK"
BASE COUNT          276 a          575 c          662 g          343 t
ORIGIN      
        1 gtgcgccctg ggtgagctgc acgctgttct gcaggaagat cgacatgccg atcgcggaaa
       61 tgagcggcgc cagccgggtc gagccgcgca gcgggcggta ggcggtgcgc tcgaccgccc
      121 agccgtaggc cgaggtgaag aagatcgaca cgatcagcac gaaggtcagc gcgagcggga
      181 tcgaggtgac gtcggccgcg gcgaggatgg tgaaggcggt gaccgcgatg aaggcgctca
      241 ccatgaagat gtcgccatgg gcgaagttga tcatgccgat gatgccgtac accatcgtgt
      301 agccgatggc gatcaggccg tacatggcgc cgagcgtgag cccgttgatc agttgctgga
      361 gtgcataggc cacgatgtct cctgctgtgg cggtgcggat ggcgcggatc ccgctcgggg
      421 ccgcgtccct gtaggcgcgg cgcaagccgc gatcaagcgc tggatcggaa gtcgtcgctc
      481 gcaacccgtc gccggccccc tgtctccttg ttggccgacg ggggcacgga caccggcgcc
      541 gcgattgcgg cgccgaagac tcgggtggga cgggcttgcg acttacttcg cgtagtcgta
      601 cttgccgccc tgccagcgat agaccacgaa gcccggcgcc ttctggtcgc ccttggcgtc
      661 gaaggcgaga tctccgatca cggtcttgaa gctgccgccg cgcatcgcct tctcgaggtc
      721 ggcgtacttg gtgctcttgg ccgtggtggc ggcctgctcg aagagctgca tcgcggcgta
      781 ggcgtagagc acgtagcctt cgggctcggt cttggcggcg cggaacttct ccaccaccgg
      841 cgcggcgtcc gggttcttgc gcgcgtcggg cgagaacgtg aacatcacgt tgtcggcggc
      901 cgggccggcg gcggtgacga gctcggagtt ggtcatcgtg tcgccgccca tcacggtgag
      961 ctgcaggccg gcggcctggg cctggcgcag gatcagcgcg acctcggtgt ggtagccgcc
     1021 gtaggccatg acctcgacgc cggcctgctt gagcttggtg acgatgccag agtagtcctt
     1081 ctcgccggcg gtgatggatt cgcgcagcgc gggcttcagg cccttgcctt ccatcgcctt
     1141 ggccatctcg tcggcgagcc ccttgccgta ggcgctcttg tcgtcgacca cggcgacctt
     1201 cttgcccttg aagtggtcgg cgatgtagcc gccggccacc aggccctgct ggtcgtcgcg
     1261 acccatgatg cggaagatgt tcttcaggcc gcgctcggtc acctgcggat tggtcgacac
     1321 ggtggccatc gggatgtcgg cctcgacata gacctcggag gccgggatcg tcgagctcga
     1381 gcaccagtgg ccgtgcatga acacgatgcc cttgttcacc atggtgttgg ccaccgagac
     1441 ggcctgcttc ggatcgcagg cgtcgtcgcc gacctcgagc ttcagtttct ggccgagcac
     1501 gccgccgcgg gcgttgatgt cggcgatggc catctccgcg cccttgcgga tctggtcgcc
     1561 ggcggaggcg tactggccgg tcatcggacc ggcgatggcg accgcgaggt cggccagggc
     1621 ggcattcgaa aagccgccga gtgcgaggac ggcggtggcg agcagggtct ttttcatgaa
     1681 acgtctcctt cgtggttgtg gttttgggcc ttggacgggg cagacggcgc caaatggggc
     1741 catctgcgtc cggcaaaggt aagcgaattt ttcaaaattg ccatccgtga tggccctagc
     1801 tgcgactacc gcgcaggttc gttgtcatcc agcacccctc cgggggcatc cggaag
//
LOCUS       DAOQNN010000573         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_55645_length_1852_cov_6.829716, whole genome shotgun sequence.
ACCESSION   DAOQNN010000573 DAOQNN010000000
VERSION     DAOQNN010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55645_length_1852_cov_6.829716"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="PKV97_17460"
     CDS             <1..1482
                     /locus_tag="PKV97_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNB07727.1"
                     /translation="YGAIVLGFVAALLGGTRMQISGPTGPMTVVFASALAVVGGDAAL
                     AMAAVLVGGLVQIGLGVLRTGGLVRFIPYPVVSGFMSGIGVIIVLLQSAPLLGVAPAS
                     SPLAAVLALPGVLAKVNAEALLLGGLTLLIVFRTPMTVSRIVPAPLVALVAMSALSAV
                     ADLQVPVIGDIPAGLPVPALPSFTLETWTTVLLLGITLGVLGSIDSLLTSLVADSITR
                     TRHHSNRELVGQGIGNLLCAFVGGLPGAGATMRTVINIKSGGRGRASGVVHSLFLLAL
                     LLGLEPFAERIPLAVLAGILIKVGVDILDYRMLRRVRTAPRADVAVMAAVFALTVLVD
                     LIVAVGAGVILSMALIIHRLARQSSLRVRPLPHAEEGGQTPAGDELPGLDSGVRVIEI
                     EGAFFFGSASQLLDRVDQVIGTRVVVFDCSRVPFMDLSAQFALEEMIEGLQAQSIAAR
                     VVVPPEIHAQLLRLGAPQLPAAILHDELDHALAEARRLAAAMP"
     gene            complement(1479..>1852)
                     /locus_tag="PKV97_17465"
     CDS             complement(1479..>1852)
                     /locus_tag="PKV97_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNB07728.1"
                     /translation="AGCVLRECWFEPTFHKHAGKLCNGVHIHVEDPAHYEHAAFRPWR
                     VQALAFKAIRRLWPDYPLWRDFPYEYEHERLAIDLINGSPLLREWVDDPAATPTALDA
                     LARPDEAAWLETRRPFLLYPG"
BASE COUNT          211 a          658 c          679 g          304 t
ORIGIN      
        1 tacggtgcga tcgtgctcgg cttcgtcgcc gcgctgctcg gcggcacccg catgcagatc
       61 tccggcccta ccgggccgat gaccgtggtg ttcgcctccg cgctcgcggt ggtgggcggc
      121 gatgccgcgc tggcgatggc ggcggtgctg gtgggcgggt tggtgcagat cggcctcggc
      181 gtgctgcgca ccggcgggct ggtgcgcttc atcccctatc cggtggtctc cggcttcatg
      241 agcggcatcg gcgtgatcat cgtgctgctg cagagcgcgc cgctgctcgg cgtggcgccg
      301 gcgtcctcgc cgctggccgc ggtgctcgcg ctcccgggcg tgctcgccaa ggtgaacgcc
      361 gaggccctgc tgctcggcgg gctcaccctg ctgatcgtgt tccgcacccc gatgacggtg
      421 agccggatcg tgcctgcacc cctggtcgcg ctggtggcga tgagcgcgct gtccgccgtc
      481 gccgacctgc aggtgccggt gatcggcgac atccccgccg ggctgcccgt accggcgctg
      541 ccgagcttca cgctggagac ctggaccacc gtgctgctgc tcggcatcac cctcggggtg
      601 ctcggcagca tcgacagcct gctgacctcg ctggtcgccg actcgatcac gcgcacccgt
      661 caccactcca accgcgagct cgtcggccag ggcatcggca acctgctgtg cgccttcgtc
      721 ggcggcctgc cgggcgcggg cgcgaccatg cgcacggtca tcaacatcaa gtcgggcggg
      781 cgcggccgtg cctcgggcgt ggtgcattcg ctcttcctgc tcgccctgct gctcgggctg
      841 gagccgttcg ccgagcgtat cccgctcgcg gtgctcgccg gcatcctgat caaggtcggc
      901 gtggacatcc tcgactaccg catgctgcgc cgggtgcgca cggcgccgcg cgccgacgtc
      961 gcggtgatgg cggcggtgtt cgcgctcacc gtgctggtgg acctgatcgt ggcggtgggc
     1021 gccggcgtga tcctgtcgat ggcgctgatc atccaccgcc tcgcccgcca gagcagcctg
     1081 cgcgtccgtc cgctccccca tgcggaggag ggcggtcaga cacccgccgg cgacgagctc
     1141 cccggcctcg acagcggcgt gcgcgtgatc gagatcgagg gcgccttctt cttcggctcg
     1201 gcgagccagc tgctcgaccg cgtggaccag gtgatcggca cccgcgtcgt cgtcttcgac
     1261 tgcagccggg tgcccttcat ggacctgtcg gcgcagttcg cgctggagga aatgatcgag
     1321 ggcctgcagg cgcagtcgat cgccgcgcgc gtggtggtgc cgcccgagat ccacgcccag
     1381 ctgctgcgcc tgggtgcgcc gcagctgccc gcggcgatcc tgcacgacga gctcgaccac
     1441 gcgctcgccg aggcgaggcg gctggccgcg gcgatgcctt aacctgggta gagcaggaag
     1501 gggcggcgcg tctccagcca cgccgcctcg tcggggcggg cgagcgcgtc gagcgcggtc
     1561 ggcgtcgccg cggggtcgtc cacccactcg cgcagcaaag gcgagccgtt gatcaggtcg
     1621 atggcgaggc gctcgtgctc gtactcgtag gggaagtcgc gccacagcgg gtagtcgggc
     1681 cacagcctgc ggatcgcctt gaaggcgagc gcctgcaccc gccacgggcg gaaggcggcg
     1741 tgctcgtaat gcgcggggtc ctcgacgtgg atgtgcacgc cgttgcacag cttgccggcg
     1801 tgcttgtgga aggtgggctc gaaccagcac tcgcgcagca cgcagccggc ga
//
LOCUS       DAOQNN010000574         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_55646_length_1852_cov_6.723428, whole genome shotgun sequence.
ACCESSION   DAOQNN010000574 DAOQNN010000000
VERSION     DAOQNN010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55646_length_1852_cov_6.723428"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKV97_17470"
     CDS             complement(<1..638)
                     /locus_tag="PKV97_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07729.1"
                     /translation="MTEVRSHAASRAAGEAAQHYRTALATGRATLDADELARLLAAAD
                     LHTTTAPADAIELSIRVHATREFGLVLSAGAGGLDGALDPANFARDRAAVHAAVELTD
                     GEDFLERFRRTIAWQRITALAWPVLIAQTGLHEAYPSSGGHLLPYPFDREPWPPRVPI
                     DLSRALRAQRDRLGALPGSAPVRWVPIDLTVPEDGYEPVNYGLDALWAAIEA"
     gene            complement(689..>1852)
                     /locus_tag="PKV97_17475"
     CDS             complement(689..>1852)
                     /locus_tag="PKV97_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB07730.1"
                     /translation="LRGLADLARFPFTAKQDLRDNYPYGLFAVPMAKIVRVHASSGTT
                     GRPTVVGYTKGDIERWAAVMARSLRVAGASCEDIILNSYGYGLFTGGLGAHYGGEALG
                     ATVIPMGGGNTEKQIQLIQEFRPTVIMATPSYMLTLADTMQRMGLDPSATTLRLGMFG
                     AEPWTNQMRAEIERVLGIDAIDVYGLSEVMGPGVACEFVGHKDGPHIWEDHFYPEIID
                     PVTGEVLPDGERGELVFTSLTKEAMPVIRYRTRDLTRLLPGSGCAMRRIDKITGRSDD
                     MLIIRGVNVFPTQIEEVLLRHDKLCGHYQLVLTREGHLDQLTVRAELRPELADGIGQG
                     LRRQIGEEVRHAIKSFVGISAEIEILDPDSIERTQVGKAKRVIDRRNAATSTA"
BASE COUNT          256 a          599 c          694 g          303 t
ORIGIN      
        1 atggcttcaa tcgcggccca aagcgcgtcc aggccgtaat tcacgggttc gtagccgtcc
       61 tccggcacgg tcagatcgat gggaacccag cgcaccgggg ccgaaccggg caacgccccc
      121 agtcggtcgc gttgcgcgcg cagggcgcgg ctcagatcga tgggaacccg aggcggccac
      181 ggctcacgat caaaaggata cggcagcagg tgcccgccac tggatggata cgcctcgtgc
      241 aaaccggtct gggcgatcag caccggccag gcgagggcgg tgatgcgctg ccaggcgatc
      301 gtgcgacgga aacgctcgag gaagtcctcg ccgtcggtga gctcgaccgc ggcatgcact
      361 gccgcgcggt cgcgcgcgaa gttggcgggg tcgagcgcgc cgtcgaggcc gccggcgccg
      421 gcgctgagca ccaggccgaa ctcgcgcgtg gcatggacgc ggatcgacag ttcgatcgcg
      481 tccgctggcg cggtcgtggt gtgcaggtct gcggcggcga gcaggcgggc gagctcgtcg
      541 gcgtccagcg tcgcgcgccc ggttgcgagg gccgtgcggt agtgctgcgc ggcctcccct
      601 gctgcgcggc tggcggcatg gcttcggact tcggtcatcg ggtgtctccg tgtctgttct
      661 tgtggggtga tgcgttcccg gcgcgccctc aggcggtgct ggttgcggcg ttgcggcggt
      721 cgatcacgcg ctttgccttg ccgacctggg tgcgctcgat gctgtcgggg tcgaggatct
      781 cgatctcggc gctgatgccg acgaagctct tgatcgcgtg ccggacctcc tcgccgatct
      841 ggcggcgcag gccctggccg atgccgtcgg cgagctcggg gcgcagctcg gcgcggacgg
      901 tgagctggtc gaggtgaccc tcgcgggtga gcacgagctg gtagtggccg cacagcttgt
      961 cgtggcgcag cagcacctcc tcgatctggg tcgggaagac gttgacgccg cggatgatca
     1021 gcatgtcgtc gctgcgcccg gtgatcttgt cgatgcggcg catcgcgcag ccgctgccgg
     1081 gcagcaggcg ggtgagatcg cgggtgcggt agcggatcac cggcatcgct tccttggtga
     1141 gcgaggtgaa caccagctcg ccacgctcgc cgtcgggcag cacctcgccg gtgaccgggt
     1201 cgatgatctc gggatagaag tggtcttccc agatgtgcgg gccgtccttg tgaccgacga
     1261 actcgcaggc cacccccggg cccatcactt cggacaggcc atacacgtcg atcgcgtcga
     1321 tgcccagcac gcgctcgatc tcggcgcgca tctggttggt ccacggctcg gcgccgaaca
     1381 tgcccagccg cagcgtggtc gccgaggggt cgagccccat gcgctgcatc gtgtcggcca
     1441 gtgtcagcat gtaggacggc gtggccatga tcaccgtggg gcggaactcc tggatgagct
     1501 ggatctgctt ctcggtgttg ccgccgccca tcgggatcac cgtggcgccg agcgcctcgc
     1561 caccgtagtg ggcgccgagg ccgccggtga agaggccgta gccgtaggag ttgaggatga
     1621 tgtcctcgca gctcgcgccg gccacgcgca gcgagcgcgc catgaccgcg gcccagcgct
     1681 cgatgtcgcc cttggtgtag ccgaccacgg tggggcggcc ggtggtgccg gacgaggcat
     1741 gcacgcgcac gatcttggcc atcggcaccg cgaagaggcc gtagggatag ttgtcgcgca
     1801 ggtcctgctt ggcggtgaag gggaacctcg ccaggtcggc caggccgcgc ag
//
LOCUS       DAOQNN010000575         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_56367_length_1838_cov_7.380258, whole genome shotgun sequence.
ACCESSION   DAOQNN010000575 DAOQNN010000000
VERSION     DAOQNN010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56367_length_1838_cov_7.380258"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /gene="folE2"
                     /locus_tag="PKV97_17480"
     CDS             complement(<1..672)
                     /gene="folE2"
                     /locus_tag="PKV97_17480"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNB07731.1"
                     /translation="MNAPEKFFLPDVQSSADHRALAIQQVGVRGLRYPMAVRDLDGGV
                     QHTVATLEMTVGLPAEVKGTHMSRFVELLEAERDALDLDGLRGLFADMLVRLEAESGR
                     IEARFPWFMKKAAPVSGVESLLDIDARVVVEKTAGGEIRSTLEAVVPVTSLCPCSKKI
                     SAYGAHNQRSHITLSATLRGDLGLRELVRMAEEEASCEVFGLLKRPDEKWVTERAYDN
                     PKFVED"
     gene            complement(669..935)
                     /locus_tag="PKV97_17485"
     CDS             complement(669..935)
                     /locus_tag="PKV97_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07732.1"
                     /translation="MNAKMLSRRAGILSAATVLVSFGISAASAAPPQVVAAAAAVAPA
                     GPVVDNTVATPAPASIQTPLELLLFVHRADLPLRPLPTKQKDHS"
     gene            1213..>1838
                     /locus_tag="PKV97_17490"
     CDS             1213..>1838
                     /locus_tag="PKV97_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNB07733.1"
                     /translation="MHMLTEGLKLAAGRALEGLLERGTATPVRGLGAGMTMTALVQSS
                     TAVTVASIGFVNTGLLSLQNAMWVIFGSNVGTTLNAWLVAALGFSFRIDAFALPFVGI
                     GAILMLAGRTLRQRALGQALAGFGVLFLGIDALKDTFSGFGAAMNLQDHIAPGLVGWL
                     ILVGIGTLLTVLMQASGAVIAIIITAAQGGLMSIEAACAMVIGTNIGTT"
BASE COUNT          273 a          629 c          637 g          299 t
ORIGIN      
        1 gtcctcgacg aacttggggt tgtcgtaggc gcgctcggtg acccacttct cgtccgggcg
       61 cttgagcagg ccgaagactt cgcacgaggc ttcttcctcg gccatgcgca ccagctcgcg
      121 caggccgagg tcgccgcgca gcgtggccga cagggtgatg tgcgagcgct ggttgtgcgc
      181 gccgtaggcg gagatcttct tggagcacgg gcacaggctg gtgaccggca ccaccgcctc
      241 gagggtgcta cggatctcgc cgcccgcggt cttttccacc accacgcggg cgtcgatgtc
      301 gagcaggctt tcgacgcccg aaaccggcgc ggccttcttc atgaaccagg gaaagcgcgc
      361 ctcgatgcgg ccgctctcgg cttcgagccg caccagcatg tcggcgaaca ggccgcgcag
      421 accgtcgaga tcgagcgcgt cgcgctcggc ctcgagcagc tcgacgaagc gcgacatgtg
      481 ggtgcccttc acctcggcgg gcaggccgac cgtcatctcc agcgtggcga cggtgtgctg
      541 cacgccgccg tcgagatcgc gcaccgccat ggggtagcgc agaccgcgca cgccgacctg
      601 ctggatcgcc agcgcgcggt ggtcggcgct ggattgcacg tcgggcagga agaatttttc
      661 cggggcgttc atgaatgatc cttttgcttg gtggggaggg ggcgcagcgg aagatccgcg
      721 cggtgcacga agagcaggag ttcgagcggg gtctggatcg aggccggggc cggggtcgcg
      781 accgtgttgt ccacgacggg cccggcagga gcgacggcag ctgccgccgc gacgacctgg
      841 ggcggtgcgg ccgaagccgc ggagatcccg aaggagacga gcaccgtagc ggcggagagg
      901 atgccggcgc gcctggagag cattttcgcg ttcatggccg tgtcctggac aagcgtggat
      961 aacgattcca cgataggcga gcaccgcagg gctgtcaaga atttgtccag gacaaattga
     1021 aattgtttgc catccggtcc aaagcgcgat ggtccccgcc gcgaacaatg ccctccccgc
     1081 ccgcgcgatc cggcattacg ctgtcgtgac gcccccttcc cgcacgagag gcccgccctg
     1141 gacaccagcc cgctcgccat ctatccgatc gtcgccggcc tgctcggcgg catcggcctg
     1201 ttcctgctcg gcatgcacat gctcacagag ggcctcaagc tcgccgccgg ccgcgcactc
     1261 gagggcctgc tcgagcgcgg caccgccacg ccggtgcgcg gcctcggcgc cggcatgacc
     1321 atgaccgcgc tggtgcagtc ctccaccgcg gtcacggtgg cgagcatcgg cttcgtcaac
     1381 accgggctgc tgtcgctgca gaacgcgatg tgggtgatct tcggctccaa cgtcggcacc
     1441 acgctcaacg cctggctggt ggcggcgctc ggcttcagct tccggatcga cgccttcgcc
     1501 ctgcccttcg tcggcatcgg cgcgatcctg atgctggcag gccgcacgct gcgccagcgc
     1561 gcgctcggcc aggcgctggc cggcttcggc gtgctcttcc tcggcatcga cgcgctcaag
     1621 gacaccttct cgggcttcgg cgcggcgatg aacctgcagg accacatcgc gcccggcctc
     1681 gtcggctggc tgatcctggt cggcatcggc accctgctga cggtgctgat gcaggcctcg
     1741 ggcgcggtga tcgcgatcat catcaccgcc gcccagggcg ggctgatgtc gatcgaggcg
     1801 gcgtgcgcga tggtgatcgg caccaacatc ggcacgac
//
LOCUS       DAOQNN010000576         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_56428_length_1837_cov_10.875982, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000576 DAOQNN010000000
VERSION     DAOQNN010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56428_length_1837_cov_10.875982"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1670
                     /locus_tag="PKV97_17495"
     CDS             3..1670
                     /locus_tag="PKV97_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB07734.1"
                     /translation="MASIQYPENADLRSLLRFAAEDGLIWLGEHRMLLLHAGAMAELR
                     KELIESVGREQARRILTRMGFASGMRDAELARKTRSASGLADAFVMGPQLHMLEGCAR
                     VEPVRLEFGGADNGFYAEFLWENAWEAEAHVQAFGESAEPVCWMMIGYASGYTSAFTG
                     RFILFRETECAATGHEHCRIVGRPAEDWPDADTLLPYFQADSIVGRLLELREEIEAMR
                     RTISCPRRTPDLIGSSAAFLHAYDLIGRAASTNVSVLLLGETGVGKERFARTLHEMSA
                     RKDEAFVAVNCAALPDELIESELFGVERGAFTGAHTSRAGKFERAHGGTLFLDEVGEL
                     PLPAQAKLLRALQEGEIERLGDERTRKVNVRLVAATNVDLQKAVNAGTFRRDLYYRLN
                     VYPVTIPPLRERTGDIPALVEAMVRRFETLHGKRIAGLSDKAMHALKLHAWPGNVREL
                     ENVLERGVILAPQNGQIEVEHLFIGTLDEHAREHSLSAQGGLETARRAQQDLAAAVLD
                     SGMSLDAFEQRLLQHAVERANGNLSAAARLLGMTRPQLNYRIKKQGA"
BASE COUNT          308 a          668 c          606 g          255 t
ORIGIN      
        1 ccttggccag catccaatac ccggagaacg ccgacctgcg cagcctgctg cgcttcgccg
       61 cagaggacgg cctcatctgg ctcggcgagc accgcatgct gctgctgcac gccggcgcga
      121 tggccgagtt gcgcaaggag ctgatcgaat cggtcggccg cgaacaggca cgccgcatcc
      181 tcacccgcat gggcttcgcc tcgggcatgc gcgacgccga actcgcacgc aagacgcgca
      241 gcgcaagcgg cctggccgac gctttcgtga tgggaccgca gctgcacatg ctcgagggct
      301 gtgcccgcgt cgagcccgta cggctggagt tcggcggtgc ggacaacggc ttctacgccg
      361 aattcctgtg ggagaacgcc tgggaggcag aagcgcacgt gcaggccttc ggcgagtccg
      421 ccgagccggt gtgctggatg atgatcggct acgcttccgg ctacaccagc gccttcaccg
      481 gacgcttcat cctcttccgc gagaccgaat gcgccgcgac cgggcacgag cactgccgca
      541 tcgtcggccg gccggccgag gactggccgg acgcggacac cctcctgccc tacttccagg
      601 ccgactcgat cgtcggccgc ctgctcgagc tgcgtgagga gatcgaggcc atgcgccgca
      661 cgatctcctg tccgcgccgc acccccgacc tcatcggcag ctccgccgcc ttcctgcacg
      721 cctacgacct catcggtcgc gccgcgtcca cgaacgtgtc ggtgttgctg ctcggcgaga
      781 ccggcgtcgg caaggagcgc ttcgcccgta ccctacacga gatgagcgcg cgcaaggacg
      841 aggccttcgt cgcggtcaac tgcgccgcgc tgcccgacga gctgatcgaa tccgagctct
      901 tcggcgtcga acgcggcgcc ttcaccggcg cgcacacctc gcgcgcgggc aagttcgaac
      961 gcgcgcatgg cggcaccctc ttcctcgacg aggtcggcga gctgccgctg cccgcgcagg
     1021 ccaaattgct gcgtgcgctg caggaaggcg agatcgagcg cctcggcgac gagcgcacgc
     1081 gcaaggtcaa cgtccgcctc gtcgccgcga ccaacgtcga cctgcagaag gcggtcaatg
     1141 ccggcacttt ccggcgcgac ctgtactacc gcctcaacgt ctatccggtc accatcccgc
     1201 cgctgcgcga gcgcaccggc gacattcccg cactggtcga ggccatggtg cgccgcttcg
     1261 aaaccctgca cggcaagcgc atcgccggcc tcagcgacaa ggcgatgcac gcactcaagc
     1321 tgcacgcctg gccgggcaac gtgcgcgagc tcgagaacgt gctcgagcgc ggcgtgatcc
     1381 tcgccccgca gaacggccag atcgaggtcg agcacctctt catcggcacg ctcgacgagc
     1441 acgcccgcga gcactcgctc agcgcccagg gcgggctcga aaccgcgcga cgcgcgcagc
     1501 aggatctcgc cgcggcggta ctcgactcgg ggatgtcgct cgacgccttc gagcagcgcc
     1561 tgctgcagca cgcggtcgag cgcgccaacg gcaacctgtc ggcggcggcg cgactgctgg
     1621 gcatgacgcg gccgcagctg aactaccgca tcaagaagca gggggcgtaa ggcgcgagga
     1681 ggggcgcatg cgggcccctc cccgaggacc aacggaagca tgccagttgc ggccgcagac
     1741 ggaaaaaagc caccgcacgt aaaccgtacg gtggcttttt tggtggcgga gtggacggga
     1801 ctcgaacccg cgacccccgg cgtgacaggc cggtatt
//
LOCUS       DAOQNN010000577         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_56581_length_1834_cov_8.207982, whole genome shotgun sequence.
ACCESSION   DAOQNN010000577 DAOQNN010000000
VERSION     DAOQNN010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56581_length_1834_cov_8.207982"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKV97_17500"
     CDS             <1..415
                     /locus_tag="PKV97_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07735.1"
                     /translation="ESFIVTALRSWMDMEVRRRRAEMRFTGVEAISLDSTTVDCGDGE
                     CIPMIASIATDQANQRLRLDSRDLASDLEFREALALVHARLTKAELRFMRDVVDRGVA
                     GAARKRRISRRQVLVRLASIRERFAEKRADDSSAA"
     gene            671..1773
                     /locus_tag="PKV97_17505"
                     /pseudo
     CDS             671..1773
                     /locus_tag="PKV97_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611204.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          297 a          594 c          681 g          262 t
ORIGIN      
        1 cgagagcttc atcgtcaccg cgctccgcag ctggatggac atggaggtcc gccgacgtcg
       61 cgccgagatg cggttcaccg gcgtcgaggc aatctcactc gacagcacga cggtcgattg
      121 cggcgatggc gagtgcattc ccatgatcgc cagcatcgcc accgaccagg ccaatcagcg
      181 cttgcgcttg gactcacgag acctcgcgag cgatctggag ttccgcgaag cgctcgctct
      241 cgtgcacgcg cggctcacga aggcggagct ccgcttcatg cgtgatgtcg tggatcgcgg
      301 cgtcgccggc gctgcccgca aacgccgcat ttctcgccgc caagtcctcg ttcgcctcgc
      361 ctcgatccga gagcgctttg ctgaaaagcg ggcagatgac tcttccgctg catagggaac
      421 caccagaggc ccatgcgtgg gcacgctgcg gaaccgtcgg agcggagcgc ccgtcccacc
      481 ccctctcccc ctgggctggg cgctccgcca accggcggct cgacggattc ggtccccact
      541 cggagaagca caccatgcga gacactcggt tcaggtttga gttcgcggct gacgtgtgac
      601 aggggtgcgc tgaacaggtc caccccttat gagagacttt cgcggcccga tgggccgaga
      661 ggagtctccg atgaagcgtc cgacgaacag gaagcgccac cgtcccgagg aggtggtcgc
      721 gaagctgagg caggctgacg aggcgctcgc caagggcgcg ccgatcgccg aggtggcgag
      781 gtcgcttggc gtgtccgagg tgacgctgca ccgctggcgc gccgagtacg gggcggtgga
      841 ccgcgacgcg gtgaagcggc tgaaggacct ggagaaggag aacgcccggc tgaagcgcct
      901 ggttgcggac cagcagctgg acatccagat cctgaaggag atcgccaagg gggaattctg
      961 agcccggcgc ggcggcgccg ggcgatcgag cacgtgacga tggcgatgga cgtctccgag
     1021 cggcgtgtct gccgcgtggc tggccagcac cggagcacgc agcgccgcgc ggcgccgccg
     1081 aacccgtacc gggacaggct ggtggccagg atgcgggagc tggccgtcgc gaacccgcgc
     1141 cgcggccggc gctacgtcat ggatttgctg cgcaaggagc gctggtcggt cggcgcgcgg
     1201 ctgatgaagc ggctttggag gcaggaaggc ctgctggtgc cgcagaggcg cgtgaaacgc
     1261 aggcggatcg gcaggggcga gaacggcatc gtgcgtcgcc gtgcgacgcg caggaacgag
     1321 gtgtggggca tggacttcgt gcaggaccgg accgcggacg gccgcccctt ccgcatgctg
     1381 gtggtgctgg acgagcacac gcgggagtgc ctggcgatcg aggtgcaccg gcacctgcgg
     1441 ggcgaggaca tcgtggcggt gctggacgag ctgacggcga tccgcggcgc gccggcgcac
     1501 atccgcgccg acaacggtcc ggagatgatc tcgaaggcgg tgaaggcctg gtgcgaggag
     1561 agcggcgccg gcgcgctgta catcgacccg ggatcgccct ggcagaacgg gatcgtggag
     1621 agcttcaacg gccggctgcg cgacgagctg ctctcatcgg agatcttcga cacgctggcg
     1681 gaggcgcggt acctgatcga ccggtggcgg ctctactaca accaccggcg gatccagcgt
     1741 gcgctgggga agctgacccc ggcggccttc gccgcggcgt gcccggcgct gcctccgctc
     1801 cggctcgctg cgctcgcctg cgctgcggca ccgc
//
LOCUS       DAOQNN010000578         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_57106_length_1825_cov_10.153672, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000578 DAOQNN010000000
VERSION     DAOQNN010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57106_length_1825_cov_10.153672"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..524)
                     /locus_tag="PKV97_17510"
     CDS             complement(3..524)
                     /locus_tag="PKV97_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNB07736.1"
                     /translation="MSTLSIDRHDLALLDALQRSGDATNATLGEAVHLSASQVSRRLQ
                     RLQEAGLITGYAALLDPAALGLDVMAFAHVVLERHGGVRTEAFEDGVAALPEVLECFS
                     VSGNADYVLRIVAPDLAAFSELMMKRVLRLPGIANIKTDIVLGKVKQTHVLPLEHIAR
                     PGRPRRGVRFTVS"
     gene            644..>1825
                     /gene="ipdC"
                     /locus_tag="PKV97_17515"
     CDS             644..>1825
                     /gene="ipdC"
                     /locus_tag="PKV97_17515"
                     /EC_number="4.1.1.43"
                     /EC_number="4.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate/phenylpyruvate decarboxylase"
                     /protein_id="HNB07737.1"
                     /translation="MNLTESLLHALIDHGARQIFGIPGDFALPYFRIIEQTGILPLHT
                     LSHEPGVGFAADAAARVQGGIGVAAVTYGAGALNMVNAVAAAYAEKSPLVVISGGPGL
                     GESQSGLLLHHQAKTLDSQLRIFQEITCDQVRLDDAARAPADIARVLGNCVRNSLPVY
                     IEIPRDMVARPCAPVVREAPRAVDADALAACVDEILARLRAARAPVLMAGVEVRRFGL
                     EDKVAELSRRLGIPVVTSFMGRGLLADQDAPLMGTYMGLAGLPEVSALVEDSDGLFLV
                     GVIISDTNFAVSGKHIDLRHTIQALEARVTMGYHTYADIPLDALIDGLLARVPHADAH
                     FTVDRPAFPHGLQADEATIAPADIACAVNDLMAAHGKLPIASDMGDCLFTAMDIEHTA
                     LV"
BASE COUNT          286 a          652 c          619 g          268 t
ORIGIN      
        1 cttcagctca cagtgaagcg cacgccgcgg cggggccggc cggggcgggc gatgtgctcg
       61 agcggcagca cgtgggtctg cttgaccttg ccgagcacga tatcggtctt gatgttggcg
      121 atgccgggca ggcgcagcac gcgcttcatc atcagctcgg agaaggcggc gaggtcgggc
      181 gcgacgatgc gcagcacgta gtccgcattg ccgctcaccg aaaaacactc cagcacctcc
      241 ggcaaggccg cgaccccgtc ctcgaaggcc tcggtgcgca cgccgccgtg gcgctcgagc
      301 accacatggg cgaacgccat cacgtccagc ccgagcgcgg cagggtcgag cagcgccgcg
      361 tagccggtga tcagcccggc ctcctgcagg cgctgcagac gacggctgac ctgcgaggcg
      421 gacaggtgca ccgcctcgcc gagcgtggcg ttggtcgcgt cgccgctgcg ctgcagtgcg
      481 tcgagaagcg caagatcgtg gcggtctatg ctcagggtgc tcatcatccg gcctcagaat
      541 gcacggaacg tgcgtatcgg cagagtttat acccgcatga tgcgcacacg gtgcggagca
      601 ggccaaccat aatgcccgtc accagaccaa ggaggagacg agcatgaacc tgaccgaatc
      661 gctgctgcac gcgctgatcg accacggcgc acgccagatc ttcggcatcc cgggcgactt
      721 cgccttgccg tatttccgca tcatcgagca gaccggcatc ctgccgctgc acacgctgtc
      781 gcacgagccc ggcgtgggct tcgccgccga cgcggccgcg cgcgtccagg gcggcatcgg
      841 ggtggcggcg gtcacctacg gcgccggcgc gctcaacatg gtcaacgcgg tcgccgccgc
      901 gtatgccgag aagtcgccgc tggtggtcat ctcgggcgga ccggggctgg gcgaatcgca
      961 atccggcctg ctgctgcacc accaggccaa gacgctggac tcgcagttgc gcatcttcca
     1021 ggagatcacc tgcgaccagg tgcggctcga cgacgcggcg cgcgcgccgg ccgacatcgc
     1081 gcgcgtgctc ggcaactgcg tgcgcaattc actgccggtg tacatcgaga tcccgcgcga
     1141 catggtcgcc cggccatgcg cgccggtagt gcgcgaggcg ccgcgcgcgg tcgatgccga
     1201 cgcgctcgcg gcctgtgtgg acgagatcct cgcacgcctg cgcgcggccc gtgccccggt
     1261 gctgatggcc ggcgtcgagg tgcgccgctt cgggctggag gacaaggtcg cagagctgtc
     1321 gcgccggctc ggcatcccgg tggtgaccag cttcatgggc cgcggcctgc tcgccgacca
     1381 ggacgccccg ctgatgggca cctacatggg gctcgccggc ctgcccgagg tgagcgcgct
     1441 ggtggaggat tccgacggac tgttcctggt cggggtgatc atctccgaca ccaacttcgc
     1501 cgtctccggc aagcacatcg acctgcgcca caccatccag gcgctcgagg cccgggtcac
     1561 gatgggctac cacacctatg ccgacatccc gctcgacgcc ctgatcgacg ggcttctcgc
     1621 gcgcgttccg cacgccgacg cgcacttcac ggtcgaccgc cccgcctttc cgcacggcct
     1681 gcaggcggac gaagcgacga tcgcgcccgc cgacatcgcc tgcgcggtaa acgacctgat
     1741 ggcggcccac ggcaagctgc cgatcgcctc cgacatgggc gactgcctgt tcaccgcgat
     1801 ggacatcgag cacaccgcgc tggtc
//
LOCUS       DAOQNN010000579         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_58269_length_1804_cov_11.580903, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000579 DAOQNN010000000
VERSION     DAOQNN010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58269_length_1804_cov_11.580903"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKV97_17520"
     CDS             <1..442
                     /locus_tag="PKV97_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNB07738.1"
                     /translation="PSTAGTLLRLVMQHPTWAARMPVGLVPDDSPEGLALIAIIDLCS
                     LGEPIPSGGLGALIERFRETPHAETLSRVAAELVEAEFDEAVVETLFEDALRKLQIDG
                     VGKEITALLQRDREQGLDAAGRHRLGELLLEKRNLASSGKVSDL"
     gene            492..>1804
                     /locus_tag="PKV97_17525"
     CDS             492..>1804
                     /locus_tag="PKV97_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HNB07739.1"
                     /translation="MAREKAKDPRKDAPAKGRPAKAKDKAAPVVESPAATPLDAEVRR
                     TRLKTLITLGKERGYLTYAEISDHLPDDVADAEQIEGIIATFNNMGIQVYDEAPAAED
                     LLMSDSVATNVDEDVAEEEAEQALSSVDSEFGRTTDPVRMYMREMGTVELLTREGEIE
                     IAKRIEDGLKHMVQAISACPTTIAEMLGIVDRIAADDAKIDELVDGLIDPNAGSAEEA
                     AAEEESADEDTEAEEDEGAEDEDEGDDEEDSAESAERANAASLLQLKNDALARFDVIR
                     GLYEKKMQALEKRGSQDLSYLALQQQISDELLNIRFTAKAIEKLCDSVRHMVEQVRSH
                     ERQILQLCVDRAGMPRQHFIKVFPGQEVNLDWLKDEIAAGKNYADGLMRIHPAVLEEQ
                     QKLIDLQDRIGIPLKELKDINRQMSTGEAKMRRAKREMTEANLRLV"
BASE COUNT          374 a          630 c          571 g          229 t
ORIGIN      
        1 cccctccacc gccggcaccc tgctgcgcct ggtgatgcag cacccgacct gggccgcgcg
       61 catgccggtc ggcctggtgc ccgacgacag cccggaaggc ctcgcgctga tcgcgatcat
      121 cgacctctgc agcctcggcg agccgatccc ctcgggcgga ctgggcgcgc tgatcgagcg
      181 cttccgcgag acaccccacg ccgaaaccct ctcgcgtgtc gccgcggaac tcgtggaggc
      241 ggaattcgac gaagcggtgg tcgagacgct gttcgaggac gccctgcgca aactgcagat
      301 cgacggcgtc ggcaaggaga tcaccgccct gctacaacgg gatcgtgagc aaggcctgga
      361 cgccgcgggc cgccatcgcc tcggagagct gctgctcgag aagcggaacc tcgcgagcag
      421 cggcaaagtc tcagatttat agtacaattt cgggtttccc cattttctta caccctcact
      481 tgaggagtgc tatggcacgc gaaaaagcca aggacccacg caaggacgcc cccgcgaagg
      541 gccgccctgc caaggccaag gacaaggcag ccccggtggt cgaaagcccc gccgccaccc
      601 cgctcgacgc ggaggtgcgc cgcacccggc tgaagacgct gatcacgctc ggcaaggaac
      661 gcggctatct cacctacgcc gagatcagcg accacctccc cgacgatgtg gccgatgccg
      721 agcagatcga aggcatcatc gccaccttca acaacatggg catccaggtc tatgacgagg
      781 cgccggccgc cgaagacctg ctcatgtcgg acagcgtcgc caccaacgtg gacgaggacg
      841 tcgccgaaga ggaagccgaa caggcgctgt cctcggtcga ctccgaattc ggccgcacca
      901 ccgacccggt gcgcatgtac atgcgcgaga tgggcacggt cgagctgctc acccgcgagg
      961 gcgagatcga gatcgccaag cgcatcgagg acggcctcaa gcacatggtg caggccatct
     1021 ccgcctgccc gaccacgatc gccgagatgc tcggcatcgt cgaccgcatc gccgccgacg
     1081 atgccaagat cgacgagctg gtcgacggcc tgatcgaccc caacgccggc agcgccgagg
     1141 aagcggccgc ggaggaggag tccgccgacg aggacaccga ggccgaagag gacgagggcg
     1201 cggaggacga ggacgagggc gacgacgagg aagacagcgc cgagagcgcc gagcgcgcca
     1261 atgccgcctc gcttctgcaa ctcaagaacg acgcgctcgc ccgcttcgac gtcatccgcg
     1321 ggctgtacga gaagaagatg caggcgctcg agaagcgtgg ctcgcaggac ctctcctacc
     1381 tcgcgctgca gcagcagatc tccgacgagc tgctcaacat ccgcttcacc gccaaggcca
     1441 tcgagaagct gtgcgactcg gtgcgccaca tggtcgagca ggtgcgcagc cacgagcgcc
     1501 agatcctgca gctgtgcgtg gatcgcgccg gcatgccgcg ccagcacttc atcaaggtgt
     1561 tccccggcca ggaagtgaac ctcgactggc tcaaggacga gatcgccgcc ggcaagaact
     1621 acgccgacgg cctgatgcgc atccaccccg cggtgctcga ggagcagcag aagctcatcg
     1681 acctgcagga ccgcatcggc atcccgctca aggaactcaa ggacatcaac cgccagatgt
     1741 ccaccggcga agccaagatg cgtcgcgcca agcgcgagat gaccgaggcc aacctgcgcc
     1801 tggt
//
LOCUS       DAOQNN010000580         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_58270_length_1804_cov_11.116066, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000580 DAOQNN010000000
VERSION     DAOQNN010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58270_length_1804_cov_11.116066"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PKV97_17530"
     CDS             <1..603
                     /locus_tag="PKV97_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HNB07740.1"
                     /translation="GQAPADGPLASLPGLGEDEPAAAPSAAAAPDAAALDRTILNASI
                     LNGALELLPQSMASMAIIPLQLRLVYRIGQAHGYELDRGHIKDFLAAAGVGLTGQYVE
                     QFGRRLIGGLLGKVLGGAGRAVGSQATGSAFSFATTWAIGKVAVQYYGGGRTVDGDAL
                     RQAFAGLFEQAKAMQERYAPEIAQRARTLDLKRLTDELRR"
     gene            848..1681
                     /locus_tag="PKV97_17535"
     CDS             848..1681
                     /locus_tag="PKV97_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB07741.1"
                     /translation="MKITPAPLLTLSNGVEMPRLGLGTWPMNDSEAAETVALALDMGY
                     RLIDTAENYENERGVGEGIRRAGVARGELFVTTKFNRKWHSVEGARQACEASLERLGL
                     DYIDLFLVHWPNPDQGRYVEAFEGLVKLQEAGLVRAIGTSNFKPAHLQQLFDRGLCPQ
                     VNQIHLDPWHARSDHIEVHEARGIVTESWSPLGRANAMLADPVILDVARRHGRTPAQV
                     ILRWHTQLGYVPIPKSSNPKRLAENLDVFGFELSAEELAAIGALDRPDPEMLDSDVFG
                     H"
BASE COUNT          271 a          637 c          618 g          278 t
ORIGIN      
        1 ggccaggcgc cggccgacgg gccgctcgcc agccttcccg gcctgggcga ggacgagccg
       61 gcggctgcgc cgtccgccgc cgccgccccc gatgcggcag cgctcgaccg gaccatcctg
      121 aatgcgtcga tcctcaacgg cgcgctcgag ctgctgccgc agtcgatggc ctcgatggcg
      181 atcatcccgc tgcagctgcg cctggtgtat cgcatcggcc aggcgcatgg gtacgagctc
      241 gaccgcgggc atatcaagga cttcctcgcc gcagcgggcg tgggcctgac cggtcagtac
      301 gtcgagcagt tcggccgccg gctgatcggc ggcctgctcg gcaaggtgct cggcggggcg
      361 gggcgcgcgg tgggcagcca ggcgaccggc tcggccttct ccttcgcgac cacctgggcg
      421 atcggcaagg tcgcggtgca gtactacggt ggcggccgca cggtcgatgg cgatgcactg
      481 cgccaggcct tcgcaggcct gttcgagcag gccaaggcga tgcaggagcg ctacgcgccc
      541 gagatcgcgc agcgtgcgcg cacgctggat ctgaagcggc tcacggatga attgcggcgc
      601 tgagccgggg agggccgcgc gagggcggtt tcacgtggaa ccgccctcgg gagtgattgc
      661 aaggcgggag atggcacgcg gagaccgccc cgccggcttg cccgtttcga ggccatgcct
      721 cggtgcgcgc tgaccctgcg acggcgccac gcctgacgga tctgcactgg cgtgtttccc
      781 agggtgctta cataagaccc cggaatgtgg aatcctgtag tccctccctc tctttctcga
      841 gattttcatg aaaatcacgc cagctcccct gctcaccttg tccaacggtg tcgagatgcc
      901 gcggctcggc ctcggcacct ggccgatgaa cgacagcgag gccgccgaga cggtcgctct
      961 cgcgctcgac atgggctatc gactgatcga caccgccgag aactacgaga acgagcgtgg
     1021 cgtcggcgaa ggtatccggc gcgcgggtgt cgcgcgcggc gagctcttcg tcaccaccaa
     1081 gttcaaccgc aaatggcaca gcgtcgaggg cgcgcgccag gcctgcgagg ccagccttga
     1141 gcgcctcggc ctggactaca tcgacctctt cctggtgcat tggcccaatc ccgaccaggg
     1201 ccgctacgtg gaggccttcg aaggcctggt gaagctgcag gaggccggcc tcgtgcgtgc
     1261 gatcggcacc tcgaacttca agcccgccca cctgcagcag ctgttcgatc gcgggttgtg
     1321 cccgcaggtg aaccagatcc acctcgaccc ctggcatgcg cgcagcgacc acatcgaggt
     1381 gcacgaggcg cgcggcatcg tcaccgagtc gtggagcccg ctcggacgcg ccaacgccat
     1441 gctggccgac ccggtgatcc tggacgtcgc gcgccgccac ggccgcacgc cggcgcaggt
     1501 gatcctgcgc tggcacaccc agctcggcta cgtgccgatc ccgaagtcgt ccaacccgaa
     1561 gcgtctcgcc gagaacctcg acgtgttcgg tttcgagctc tccgccgagg agctcgccgc
     1621 gatcggtgcg ctcgaccgcc ccgatccgga gatgctggat tcggacgtgt tcgggcactg
     1681 aggccgctgt cgcggtcggc gttcgcggct tgcgccgctc ctacgagaac cggcgcggct
     1741 cccgcttcct gcaggagcgg cgcaagccgc gaacgccacc catcgaccga tcggcgccgg
     1801 ctag
//
LOCUS       DAOQNN010000581         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_58340_length_1803_cov_7.681350, whole genome shotgun sequence.
ACCESSION   DAOQNN010000581 DAOQNN010000000
VERSION     DAOQNN010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58340_length_1803_cov_7.681350"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..>1803)
                     /locus_tag="PKV97_17540"
     CDS             complement(56..>1803)
                     /locus_tag="PKV97_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB07742.1"
                     /translation="WVNTYNQWLNNIQDWCISRQLWWGHRIPAWYDEEGRIYVATCEE
                     EAIRAWKADLQRGIDALDAEVQTRQREGQTAEQYPEIAERLALLHARHDAGRLRQEDD
                     VLDTWYSSALWPFSTLDWTAEWPEKSNDALDLYLPSTVLVTGFDIIFFWVARMVMMTK
                     HITGRIPFKHVYVHGLIRDAEGQKMSKSKGNVLDPIDLIDGIALDELIKKRTFGLMNP
                     KQAQSIEKKTRKEFPEGIPAFGTDALRFTFASLASPGRDIKFDLARCEGYRNFCNKLW
                     NATRFVLMNCEGQDCGIGETVACSTEVLDFSFADRWIVSRLQRTEAEVAEHFDAYRFD
                     LVARAVYEFVWDEYCDWYLELAKVQIQSGTPAQQRATRRTLLRVLETVLRLAHPLIPF
                     ITEELWQTVAPLAGRKEGDSIMRARYPQAEPKRIDEASEAKVAELKAMIYACRNLRGE
                     MNISPAQRLPLVAAGDKAALALYAPYLAGLAKLAEVQVVDEIGADELAPVAVAGETRL
                     MLKVEIDVAAERERLGKEIARLEGEIAKAEGKLGNASFVDRAPAAVVQQERDRLAGFK
                     ATVGKLKPQLAKLGG"
BASE COUNT          251 a          582 c          648 g          322 t
ORIGIN      
        1 ggaggaagcg cgacgcgcat ttgaacggaa tgcacgcgcg ccctccgtgc ggccatcagc
       61 cccccagctt ggcgagctgc ggcttgagct tgcccaccgt ggccttgaag cccgccaggc
      121 ggtcgcgttc ctgctgcacc accgcggccg gcgcgcggtc tacgaagctg gcgttgccga
      181 gcttgccttc ggccttggcg atctcgccct ccaggcgggc gatctccttg cccagacgct
      241 cgcgctcggc ggcgacgtcg atctccacct tcagcatcag gcgggtctcg cctgcgaccg
      301 cgaccggggc gagctcgtcg gcgccgatct cgtccacgac ctgcacctcg gcgagcttgg
      361 cgaggccggc gaggtaaggc gcgtacagcg ccagtgccgc cttgtcgccc gcggccacca
      421 gcggcaggcg ctgcgccggg gagatgttca tctcgccgcg caggttgcgg caggcgtaga
      481 tcatcgcctt gagctcggcg accttggcct cggaggcctc gtcgatgcgc ttgggctcgg
      541 cctgcgggta gcgcgcgcgc atgatgctgt cgccttcctt gcgcccggcg agcggggcga
      601 ccgtctgcca gagttcttcg gtgatgaagg ggatcagcgg gtgcgccagg cgcagcacgg
      661 tctcgagcac gcgcagcagc gtgcggcggg tggcgcgctg ctgcgccggg gtgcccgact
      721 ggatctgcac cttggcgagc tccaggtacc agtcgcagta ctcgtcccac acgaactcat
      781 acaccgcgcg cgcgaccagg tcgaagcggt aggcgtcgaa gtgctcggcc acctcggcct
      841 cggtgcgttg caggcgcgac acgatccagc ggtcggcgaa ggagaagtcg agcacctcgg
      901 tggagcaggc gacggtctcg ccgatgccgc agtcctggcc ttcgcagttc atcagcacga
      961 agcgggtggc gttccacagc ttgttgcaga agttgcggta gccctcgcag cgcgcgaggt
     1021 cgaacttgat gtcgcggccc gggctggcga gcgaggcgaa ggtgaagcgc agcgcgtcgg
     1081 taccgaaggc ggggatgccc tcggggaact ccttgcgcgt cttcttctcg atgctctgcg
     1141 cctgcttggg gttcatcagg ccgaaggtgc gcttcttgat gagctcgtcg agcgcgatgc
     1201 cgtcgatgag gtcgatcggg tcgagcacgt tgcccttgga cttgctcatc ttctggcctt
     1261 ccgcatcgcg gatcaggccg tgcacgtaca cgtgcttgaa ggggatcctg ccggtgatgt
     1321 gcttcgtcat catcaccatg cgggcgaccc agaagaagat gatgtcgaag ccggtgacga
     1381 gcacggtgga gggcaggtag aggtcgagcg cgtcgttgct cttctccggc cactcggcgg
     1441 tccagtcgag cgtggagaac ggccacagcg cggacgagta ccaggtgtcg agcacgtcgt
     1501 cttcctggcg caggcggccg gcgtcgtggc gggcgtggag cagggcgagg cgctcggcga
     1561 tctcggggta ctgctcggcg gtctggcctt cgcgctggcg cgtctgcacc tcggcgtcga
     1621 gggcgtcgat gccgcgctgc aggtcggcct tccaggcgcg gatcgcctct tcctcgcaag
     1681 ttgcgacgta gatgcggcct tcctcgtcgt accaggccgg gatgcggtgg ccccaccaca
     1741 gctggcgcga gatgcaccag tcctggatgt tgttgagcca ctggttgtag gtgttgaccc
     1801 agt
//
LOCUS       DAOQNN010000582         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_58342_length_1803_cov_6.178490, whole genome shotgun sequence.
ACCESSION   DAOQNN010000582 DAOQNN010000000
VERSION     DAOQNN010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58342_length_1803_cov_6.178490"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PKV97_17545"
     CDS             complement(<1..390)
                     /locus_tag="PKV97_17545"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HNB07743.1"
                     /translation="MDVLAPQDCFVCGATSGGEAVCAACADELPRRPSTACPQCGLPG
                     LDGGHCGACRRSAPAFDATRAVFDFAFPVDAMIHALKYRHQLAVGRYLGDALARCGAD
                     FAAEDGAGADLVLPMPLHPRRLAERGFN"
     gene            500..1480
                     /gene="bioB"
                     /locus_tag="PKV97_17550"
     CDS             500..1480
                     /gene="bioB"
                     /locus_tag="PKV97_17550"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNB07744.1"
                     /translation="MTTTSATIERPAAPPSRHQWATAEVEALFELPLLELVFRAQQVH
                     RSHFDPQEVQRSTLLSIKTGGCSEDCGYCSQSARHDSGLARQRLLPVDEVLANARAAK
                     ARGASRFCMGAAWRGPKDQDLAPVLEMVREVKALGLQTCVTLGMLKEGQAEQLADAGL
                     DYYNHNLDTAPDFYGRVITTHTLQDRLDTLDKVRGAGINVCSGGIVGMGENRRQRAAL
                     IAQLANLPAPPESVPINNLVQVPGTPLAGAGALDPFEFVRTIAAARITMPTSYVRLSA
                     GRGEMSDELQALCFLAGANSMFYGERLLTTDNPDADRDDRLFARLGLRAI"
     gene            1492..>1803
                     /locus_tag="PKV97_17555"
     CDS             1492..>1803
                     /locus_tag="PKV97_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-[acyl-carrier protein]
                     O-methyltransferase BioC"
                     /protein_id="HNB07745.1"
                     /translation="MDIPTPGQDVSARDAPPGQAPAAPGFALDRSLLRRRFERAAPHC
                     DGADALAREVARRMDERLDYIRLSPQRVLDLGCGTGADLPRLAERHPAATLLAADFAP
                     AM"
BASE COUNT          285 a          703 c          593 g          222 t
ORIGIN      
        1 gttgaagccg cgctcggcga gcctgcgcgg atgcagcggc atcggcagca cgagatcggc
       61 ccccgcgccg tcttccgcag cgaaatcggc gccgcatcgg gccaacgcat cgccaaggta
      121 acggcccacc gcgagctggt ggcggtactt gagggcatgg atcatcgcgt cgaccgggaa
      181 ggcgaaatcg aacaccgccc gcgtcgcatc gaaggccggc gcgctgcgcc ggcaggcgcc
      241 gcagtggcca ccatccaggc cgggcaggcc gcattgcggg cacgcggtgg aaggacgccg
      301 cggcagctcg tcggcacagg cggcgcagac ggcctcgccg ccgctcgtcg caccgcagac
      361 gaagcagtcc tgcggcgcga gcacgtccat cgcatggttg aacaggacgc ggaggggatg
      421 cagtgaattt gacaagattt ccaccgatgg aagaacatgc gggcctcgta attcgtaatt
      481 acagcctgga aagcattaca tgacaacgac ttccgcgacg atcgaacgcc ccgcagcgcc
      541 cccgtcccgc caccagtggg cgaccgccga ggtcgaggcg ctcttcgagc tgcccctgct
      601 cgagctcgtc ttccgcgccc agcaggtgca tcgcagccac ttcgacccgc aggaagtgca
      661 acgctccacc ctgctctcga tcaagaccgg cggctgctcg gaagactgcg gctactgctc
      721 gcagtccgcc cgccacgaca gcggtctcgc ccgccagcgc ctgctcccgg tcgacgaggt
      781 gctcgccaac gcgcgcgcgg ccaaggcgcg cggcgccagc cgcttctgca tgggtgccgc
      841 ctggcgcggg ccgaaggacc aggacctcgc cccggtgctc gagatggtgc gcgaggtgaa
      901 ggcgctcggc ctgcagacct gcgtgaccct gggcatgctg aaggaaggcc aggccgagca
      961 gctcgccgac gccggcctcg actactacaa ccacaacctc gacaccgcgc ccgacttcta
     1021 cggccgggtg atcaccaccc acaccctgca ggaccgcctc gacaccctcg acaaggtgcg
     1081 cggcgccggc atcaacgtgt gcagcggcgg catcgtcggc atgggcgaga accgccgcca
     1141 gcgcgccgcg ctcatcgccc agctcgccaa cctgccggcg ccgcccgagt cggtgccgat
     1201 caacaacctg gtccaggtcc cgggcacgcc gctcgcgggc gccggggcgc tcgacccctt
     1261 cgaattcgtg cgcacgatcg ccgccgcgcg catcaccatg ccgaccagct acgtgcgcct
     1321 gtccgccggc cgcggcgaga tgagcgacga gctgcaggcg ctgtgcttcc tggcgggcgc
     1381 caactcgatg ttctacggcg agcgcctgct caccaccgac aaccccgacg ccgatcgcga
     1441 cgaccgcctg ttcgcgcgcc tcggcctgcg cgcaatctga gcggcgagcg catggacatc
     1501 cccacccccg gccaggacgt cagcgcccgg gacgcccccc ccgggcaggc ccccgccgcg
     1561 ccgggcttcg ccctcgaccg cagcctgctg cgccgccgct tcgagcgcgc cgcgccgcac
     1621 tgcgacggcg ccgacgcgct ggcgcgcgag gtcgcgcggc gcatggacga gcgcctcgac
     1681 tacatccgcc tgagtccgca gcgcgtgctc gacctcggct gtggcaccgg ggcagacctg
     1741 ccgcgcctgg ccgaacgcca ccccgcggcc accctgctcg ctgccgactt cgcgcccgcg
     1801 atg
//
LOCUS       DAOQNN010000583         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_58733_length_1796_cov_8.807582, whole genome shotgun sequence.
ACCESSION   DAOQNN010000583 DAOQNN010000000
VERSION     DAOQNN010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58733_length_1796_cov_8.807582"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKV97_17560"
     CDS             complement(<1..334)
                     /locus_tag="PKV97_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNB07746.1"
                     /translation="MALYADTCILLPLFFRDASTDAALAWLEASASETILISPWTRTE
                     FASAAGIMARRGDISADLHREGLERFDRLVNTRLAVEAVDTTDFDRARGWIAAYRSGL
                     RAGDALHLA"
     gene            complement(334..603)
                     /locus_tag="PKV97_17565"
     CDS             complement(334..603)
                     /locus_tag="PKV97_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNB07747.1"
                     /translation="MKDLTPGFPLTLAETKSHLSAVVDQVIAGEEIVITRRGRPVARI
                     IAEPAQPAADAAAIAAELRAFVLAQAPQARTAAEVVREARDGARY"
     gene            complement(651..1262)
                     /locus_tag="PKV97_17570"
     CDS             complement(651..1262)
                     /locus_tag="PKV97_17570"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HNB07748.1"
                     /translation="MKLLASLTSPYARKIRVILAEKALPFELVVDSPWEANTRIPELN
                     PLGKVPVLITDEGETFFDSPVIAGYLETLGAPPALLPPAGLDAVRVRQTEALADGVTD
                     AAVAALLESRRPDGERSEREIARQKDKIDRGLAELERRLDGRHAFNGGPMQLGDIAVA
                     VALGYLDLRFPDVDWRSRHPGLVPFAERMFARGSLRDTQPPAG"
     gene            complement(1512..>1796)
                     /locus_tag="PKV97_17575"
     CDS             complement(1512..>1796)
                     /locus_tag="PKV97_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB07749.1"
                     /translation="LAEGIAPPRQLVLVGMAAGETTGSGRSYDTPALPKNIPALVIHG
                     EDDDTVALANVLAWARPQEQPIIVIPGADHFFHGKLHLIRELIARNVAHA"
BASE COUNT          242 a          559 c          718 g          277 t
ORIGIN      
        1 cggccagatg cagcgcatcg cccgcacgta gtccgctgcg gtaggcggca atccagcctc
       61 tggcgcggtc gaaatccgtg gtgtcgaccg cttccacggc aaggcgggtg ttgacgaggc
      121 ggtcgaagcg ctcgagcccc tcgcgatgga ggtccgcgga aatgtcaccc ctgcgcgcca
      181 tgatccccgc ggcgctggcg aactcggtgc gcgtccacgg gctgatcagg atggtttcgc
      241 tggcgctggc ttcgagccac gcgagcgccg cgtcggtgct ggcgtcgcga aagaacaggg
      301 gcagcaggat gcaggtgtcg gcatacaagg ccatcagtag cgtgccccgt cacgcgcttc
      361 acgcacgact tccgctgccg tgcgggcttg tggagcctgg gcgagcacga aggcgcgcag
      421 ctcggcggcg atggctgcgg cgtcggcggc cggctgcgcg ggttcggcga tgatgcgggc
      481 gacgggccgg ccgcggcggg tgatgacgat ctcttcgccg gcgatcactt gatcgacgac
      541 ggcggacagg tgcgatttgg tttcggcgag ggtgagggga aaacctgggg tcaggtcttt
      601 catttgctca ccccacccgt cgaaccggcg tgctcgcggc tccagcccgc ctagcccgcc
      661 ggcggctggg tgtcgcgcag gctgccgcgg gcgaacatgc gctcggcgaa gggcaccagg
      721 ccggggtggc ggctgcgcca gtcgacatcg gggaagcgca ggtcgaggta gccgagcgcg
      781 accgcgaccg cgatgtcgcc aagctgcatc gggccgccgt tgaaggcgtg gcggccgtcg
      841 aggcggcgtt cgagctcggc gaggccgcgg tcgatcttgt ccttctggcg ggcgatctcg
      901 cgttcgctgc gctcgccgtc ggggcggcgc gattcgagca gggcggcgac cgccgcgtcg
      961 gtgacgccgt cggcgagcgc ctcggtctgg cgcacgcgca ccgcgtcgag cccggcgggc
     1021 gggagcagcg cgggcggggc gccgagggtc tcgaggtagc cggcgatgac cggcgagtcg
     1081 aagaaggtct cgccttcgtc ggtgatcagc accggcacct tgccgagcgg gttgagctcg
     1141 gggatgcggg tgttcgcttc ccagggcgag tcgaccacga gctcgaaggg cagcgccttc
     1201 tcggcgagga tgacgcggat cttgcgcgcg taggggctgg tcagcgaggc gagcagtttc
     1261 atgatggcag gtctccggtg gtggccgacc cagggtacgc cgcaggcggg tggggggaaa
     1321 tcagggaaac cggaaggatc gcgggccgcc ctcccgagaa ggccggcgtg gtgcggaggc
     1381 gggcgcggcg cggattcggg cgaggcgcgg agtccggtgc tggaatcgcg acttgcgtcg
     1441 ctcctacggc aatcttgtaa gcgcctgggt ttcgtaggag cgacgcaagt cgcgattgcc
     1501 ggcggccgtg ttcaggcgtg cgcgacgttg cgcgcgatga gctcgcggat caggtgcagc
     1561 ttgccgtgga agaagtggtc tgcgccgggg atcacgatga tcggctgctc ctgcggccgc
     1621 gcccaggcga gcacgttggc gagcgccacg gtgtcgtcgt cctcgccatg gatcaccagc
     1681 gcggggatgt tcttcggcag cgccggggtg tcgtagctgc gcccgctgcc ggtggtctcg
     1741 ccggcggcca tgccgaccag cacgagctgg cgcggcggcg cgatgccttc ggccag
//
LOCUS       DAOQNN010000584         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_58808_length_1795_cov_9.276437, whole genome shotgun sequence.
ACCESSION   DAOQNN010000584 DAOQNN010000000
VERSION     DAOQNN010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58808_length_1795_cov_9.276437"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /gene="cmk"
                     /locus_tag="PKV97_17580"
     CDS             <1..996
                     /gene="cmk"
                     /locus_tag="PKV97_17580"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNB07750.1"
                     /translation="AVAALFADGPCRLRNIASWRVKETDRIAAMATELRKLGAEVEEG
                     ADYLVVQRPQRLQPAAIDTYDDHRMAMCFSLASLGGCRVRINDPKCVNKTFPGYFEAF
                     ARVARPVPVLAIDGPSASGKGTVAARVAAALGWHYLDSGSLYRLVALAAMRAGVAFDD
                     EAGVAALAAGLPARFEGGRVLLGEGADRDVTDEIRSEACSVGASKVAVLPAVRAALLD
                     RQRDYRAAPGLVAEGRDMGSVIFPDAGLKVFLTASAEARAERRHKQLIEKGLAANMES
                     LLKDLQERDARDAARPVAPLVKLPDAALLDTTQRDVDEAVAFVLDLVGRGGKSSG"
     gene            1087..>1795
                     /locus_tag="PKV97_17585"
     CDS             1087..>1795
                     /locus_tag="PKV97_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNB07751.1"
                     /translation="MSNATPAFEESFAALFEESLALQEMRAGEVITAEVVRIDQNFVV
                     VNAGLKSESYVPIEEFRNDRGELEANIGDFVHVAIDALEDGYGETRLSRDKAKRIAAW
                     NDLEKALNEGTLVKGVITGRVKGGLTVMTNSIRAFLPGSLVDMRPVKDTTPYEGKEYE
                     FKVIKLDRKRNNVVVSRRAVLEESMGEEREKLLANLKEGTVIKGVVKNITDYGAFVDL
                     GGIDGLLHITDLAWRRVR"
BASE COUNT          296 a          616 c          610 g          273 t
ORIGIN      
        1 gcggtggcgg cgctgttcgc cgacgggccg tgccggctgc gcaacatcgc gagctggcgg
       61 gtcaaggaga ccgaccgcat cgccgccatg gccaccgagc tgcgcaagct cggcgccgag
      121 gtggaggagg gcgccgacta cctggtggtg cagcgcccgc agcgcctgca gccggcggcg
      181 atcgacacct acgacgacca ccgcatggcg atgtgcttct cgctggcgag cctgggcggg
      241 tgccgtgtgc gcatcaacga cccgaagtgc gtgaacaaga ccttccccgg ctacttcgag
      301 gccttcgcgc gggtggcgcg gccggtgccg gtgctggcca tcgacggccc ctcggcctcg
      361 ggcaagggca cggtggcggc acgcgtcgcc gcggcgctgg gctggcacta cctcgacagc
      421 ggctcgctct accgcctggt ggcgctggcc gcgatgcgcg cgggagtggc cttcgacgac
      481 gaggccggtg tggccgcgct cgccgcgggt ctgccggcgc gcttcgaggg cgggcgggtg
      541 ctgttgggag agggcgcgga ccgcgacgtc accgacgaga tccgctccga ggcctgctcg
      601 gtcggcgcct cgaaggtcgc cgtgctgccg gcggtgcgtg cggccctgct cgaccgccag
      661 cgcgactacc gcgcggcccc cggcctggtg gccgaaggcc gcgacatggg ctcggtgatc
      721 ttccccgatg cagggctcaa ggtcttcctc accgcctcgg ccgaggcgcg cgccgaacgc
      781 cgccataagc agttgatcga aaagggtttg gctgctaaca tggaaagtct tctgaaagac
      841 cttcaggaac gggatgcgcg cgatgccgcc cggcctgtgg cgccactcgt gaagctgccg
      901 gatgcggccc tgctcgacac cactcaacgg gacgtcgacg aggccgtcgc cttcgttctc
      961 gatctcgtcg ggcggggggg caagtcgtcc ggttgaacgc aatcgcgcgg gagcgtgggc
     1021 tccggcgcgt tttttcttga accagccatg tttgccgtcc cacggcggtg ccggaatcca
     1081 ccttttatgt ccaacgccac ccctgccttc gaagaaagct ttgccgccct cttcgaggaa
     1141 agcctcgccc tccaggaaat gcgcgccggt gaagtcatca ccgccgaagt cgtgcgcatc
     1201 gaccagaact tcgtcgtcgt caacgccggc ctgaagtccg aaagctacgt gcccatcgag
     1261 gagttccgca acgaccgcgg cgaactcgaa gccaacatcg gcgacttcgt ccacgtcgcc
     1321 atcgacgccc tcgaagacgg ctacggcgag acccgcctgt cgcgcgacaa ggccaagcgc
     1381 atcgccgcct ggaacgacct cgagaaggcg ctcaacgaag gcaccctggt caagggcgtc
     1441 atcaccggcc gcgtcaaggg tggcctgacc gtcatgacca acagcatccg cgccttcctg
     1501 ccgggctcgc tggtcgacat gcgtccggtc aaggacacca cgccgtacga aggcaaggaa
     1561 tacgagttca aggtcatcaa gctcgaccgc aagcgcaaca acgtcgtggt ctcgcgtcgc
     1621 gccgtgctgg aagagtcgat gggcgaagag cgcgagaagc tgctcgccaa cctcaaggaa
     1681 ggcaccgtca tcaagggcgt ggtcaagaac atcaccgact acggtgcgtt cgtggacctc
     1741 ggcggcatcg acggcctgct gcacatcacc gacctggcct ggcgccgcgt ccgtc
//
LOCUS       DAOQNN010000585         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_58873_length_1794_cov_6.855089, whole genome shotgun sequence.
ACCESSION   DAOQNN010000585 DAOQNN010000000
VERSION     DAOQNN010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58873_length_1794_cov_6.855089"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /gene="rsxC"
                     /locus_tag="PKV97_17590"
     CDS             complement(<1..1370)
                     /gene="rsxC"
                     /locus_tag="PKV97_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNB07752.1"
                     /translation="MKIFRFRGGIHPEGNKDPTACHPIARLPLPQRLYIPLQQHIGEP
                     AAPEVEVGDHVLKGQRLARHQGSVSAPVHAPTSGRVIAIGDHMAPHPSGLPVQTITLE
                     PDGEERWIETEPAVDPFALTRAEVSARVRDAGIVGLGGAAFPAAVKLDAGNRHPIDTL
                     ILNGGECEPYLTCDDRLMREGAGYVVEGVRLIANGLGAKRTVIAIEDNKPEALEAMRA
                     AAAAHPGIHVLRVPSRYPMGSEKQLVTTVTGREIPAGKLSAEIGVSVHNVGTALAVCR
                     AVRFGKPLVSRVVTVGGGAIRNPRNVVVPIGTPAGDLIDFCGGFTEPPTRVLMGGPMM
                     GQVLPGLEAPVVKGTSGIIALTAAEAHAASERACLHCGRCVDVCPVGLVPLELAARVR
                     AGRIDDAAELGMGDCLSCGCCAYVCPAHLPLAHLFNYAKGEIAARREAQRKAEETRKL
                     AEARRLR"
     gene            complement(1406..>1794)
                     /locus_tag="PKV97_17595"
     CDS             complement(1406..>1794)
                     /locus_tag="PKV97_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNB07753.1"
                     /translation="GFAGCAQAAAAVAAGDAPVTLCPPGGRAVAEKLAQILGATFDPG
                     NLPDRGPLLARVRTDACIGCSRCIKSCPTDAILGATKQLHVVLEEACIGCGACAEVCP
                     TGGIDLEGIPVTLRNWRWHKPGVGHA"
BASE COUNT          230 a          629 c          691 g          244 t
ORIGIN      
        1 cgcagccggc gcgcctcggc cagcttgcgt gtctcctcgg ccttgcgctg ggcttcgcgg
       61 cgggcggcga tctcgccctt ggcatagttg aacaggtggg ccagcggcag gtgggccggg
      121 cacacgtagg cacagcagcc gcaggacagg cagtcgccca tgccgagctc ggcggcgtcg
      181 tcgatacgcc cggcacgcac gcgcgcggcg agctcgagcg gcaccaggcc gaccgggcag
      241 acatccacgc agcgcccgca atgcaggcag gcacgttcgc tcgccgcgtg cgcctcggcg
      301 gcggtgagcg cgatgatgcc gctggtgccc ttgaccaccg gcgcctccag accgggcagg
      361 acctggccca tcatcggccc gcccatcagc acgcgggtgg ggggctcggt gaagccgccg
      421 cagaagtcga tgaggtcccc ggccggcgtg ccgatcggca ccaccacgtt gcgcgggttg
      481 cggatcgcgc cgccgcccac ggtgaccacg cgcgacacca gcggcttgcc gaagcgcacc
      541 gcgcggcaca ccgcgagcgc ggtgccgacg ttgtgcacgc tgacgccgat ctcggcggag
      601 agcttgccgg cggggatctc gcgccccgtc acggtggtga cgagctgctt ctcggagccc
      661 atcgggtagc gcgagggcac gcgcagcacg tggatgccgg gatgggccgc ggcggccgcg
      721 cgcatcgcct cgagcgcctc gggcttgttg tcctcgatcg cgatcacggt gcgtttcgcg
      781 cccaggccgt tggcgatcag gcgcacaccc tcgaccacgt agccggcgcc ctcgcgcatc
      841 aggcggtcgt cgcaggtcag atagggctcg cactcgccgc cgttgaggat cagcgtgtcg
      901 atcgggtggc ggttgccggc gtccagcttg accgcggccg ggaaggccgc gccgcccagg
      961 cccacgatgc ccgcgtcgcg cacgcgcgcg gagacctcgg cacgggtgag cgcgaagggg
     1021 tcgaccgccg gctcggtctc gatccagcgc tcctcgccat cgggctcgag cgtgatcgtc
     1081 tgcaccggca ggccggaggg gtgcggcgcc atgtggtcgc cgatcgcgat caccctgccc
     1141 gacgtgggcg catgcaccgg cgccgaaacc gaaccctggt ggcgggcgag gcgctggccc
     1201 ttgaggacgt ggtcgccgac ctcgacctcg ggtgcggccg gttcgccgat gtgctgctgc
     1261 agcgggatgt acaggcgctg cggcagcggc aggcgggcga tcggatggca ggcggtgggg
     1321 tccttgttcc cttcgggatg gatgccgccg cggaagcgga agatcttcat ggtgcgtggg
     1381 gttccggtgt gcttgcggcg ggcgttcagg cgtgtcccac gcccggcttg tgccagcgcc
     1441 agttgcgcag ggtgaccggg atgccctcga gatcgatgcc gccggtgggg cagacctcgg
     1501 cgcaggcgcc acagccgatg caggcctctt cgaggaccac gtggagctgc ttggtcgcac
     1561 ccaggatggc gtcggtcggg caggacttga tgcagcgcga gcagccgatg caggcgtcgg
     1621 tgcgcacgcg cgccagcagc gggccgcggt cgggcaggtt tcccgggtcg aaggtggcgc
     1681 cgaggatctg cgccagcttc tcggcgaccg cacgcccgcc cggggggcac agcgtgaccg
     1741 gcgcgtcgcc cgcggccacc gcggcggccg cctgcgcgca gccggcgaag ccgc
//
LOCUS       DAOQNN010000586         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_59115_length_1790_cov_6.174063, whole genome shotgun sequence.
ACCESSION   DAOQNN010000586 DAOQNN010000000
VERSION     DAOQNN010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59115_length_1790_cov_6.174063"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..672
                     /gene="tadA"
                     /locus_tag="PKV97_17600"
     CDS             208..672
                     /gene="tadA"
                     /locus_tag="PKV97_17600"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNB07754.1"
                     /translation="MNDEDYMRAALEQARAAGAADEVPVGAVVVCGGEIVGRGFNQPI
                     GRHDPTAHAEIMALRDAAAHLGNYRLPGCELYVTLEPCAMCSGAIMHARIARVVFGAR
                     DPKTGVAGSVLDLFAEPRLNHHATIEGGLLADECGRMLSSFFAARRGRTLVA"
     gene            673..1152
                     /locus_tag="PKV97_17605"
     CDS             673..1152
                     /locus_tag="PKV97_17605"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNB07755.1"
                     /translation="MHLRVDLGRQCLELFGADGACIRRYAVSTGERGAGERSGSLCTP
                     RGRHRIRARIGAGAPAGAVFRGRRPTGEVWTAEFAAAHPGRDWILSRILWLCGEEPGR
                     NRGGEVDTMRRYIYLHGTGDDQPMGVARSHGCVRMRNHDIIELFELVPAGTVVEIVE"
     gene            1149..1574
                     /locus_tag="PKV97_17610"
     CDS             1149..1574
                     /locus_tag="PKV97_17610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB07756.1"
                     /translation="MKGVRIEVVGWDEAEARVMPVRTGVFVVEQGVPEEIERDAFDAV
                     CRHAIAHDAEGRVIATGRLLPDGHIGRMAVLREARGAGVGGAVLQALIAEAARRGLNE
                     VALNAQTHALDFYLRHGFEPVGEVFMEAGIPHRAMRRPV"
     gene            complement(1608..>1790)
                     /locus_tag="PKV97_17615"
     CDS             complement(1608..>1790)
                     /locus_tag="PKV97_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNB07757.1"
                     /translation="SLEWERWLSPAFIRAPGYGTRCSSVVLRDRAGRTRFVEWTWDVD
                     GELRSRVEHAFGAGTT"
BASE COUNT          230 a          627 c          684 g          249 t
ORIGIN      
        1 gctgcccgcg tcggcctcaa cccgggcgcc ctgcaggagc gacgcgagtc gcgatcttgc
       61 cattccgccc gttaccgcgc ccgatggagg ggctgagccg ccgcgttcgc gactcgcgtc
      121 gctcctgcga tgagccggcg cggcgccggc tcgagtgccc gcggcctttc gggctagaat
      181 cgcccccgtt cccgatctgc gaaaaccatg aacgacgaag actacatgcg cgctgcgctc
      241 gagcaggcgc gcgcggccgg ggccgcggac gaggtgccgg tgggcgcggt ggtggtgtgc
      301 gggggcgaga tcgtcggccg cggcttcaac cagccgatcg ggcgacacga ccctaccgcg
      361 cacgccgaga tcatggcgct gcgcgacgcc gcggcgcacc tcggcaacta ccgcctgccc
      421 ggctgcgagc tctacgtcac cctggagccc tgcgcgatgt gctcgggtgc gatcatgcac
      481 gcccgcatcg cgcgcgtggt gttcggtgcg cgcgacccca agaccggtgt cgccggcagc
      541 gtgctcgatc tcttcgccga gccgcgcctc aaccatcacg ccaccatcga gggcggactg
      601 ctcgccgacg agtgcgggcg catgttgtcg agcttcttcg ccgcccgccg cgggcgcacg
      661 ctggtggcct gaatgcacct ccgcgtggac ctcggccggc agtgcctgga gctcttcggc
      721 gccgacggcg cctgcatccg ccgctacgcg gtgtcgaccg gcgaacgcgg tgcgggcgag
      781 cgcagcggca gcctgtgcac gccgcgcggc cgccaccgta tccgtgcgcg catcggcgcg
      841 ggcgcaccgg cgggtgcggt gttccgcggg cgcaggccaa ccggcgaggt gtggacggcg
      901 gagttcgcgg ccgcccaccc ggggcgcgac tggatcctgt cgcgcatcct gtggctgtgc
      961 ggcgaggagc ctgggcgcaa ccgcggtggc gaggtcgaca ccatgcggcg ctacatctac
     1021 ctgcacggca ccggcgacga ccagcccatg ggcgttgcgc gctcgcacgg ctgcgtgcgc
     1081 atgcgcaacc acgacatcat cgagctgttc gagctcgtgc cggcgggcac ggtggtggag
     1141 atcgtcgaat gaagggcgtg cggatcgagg tggtgggctg ggacgaggcc gaggcgcggg
     1201 tgatgccggt gcgtaccggg gtcttcgtgg tggagcaggg cgtgcccgag gagatcgagc
     1261 gtgacgcctt cgacgcggtg tgccggcacg cgatcgcgca cgatgccgag ggacgggtga
     1321 tcgccaccgg gcgcctgctg cccgacggtc acatcggccg catggcggtg ctgcgtgagg
     1381 cgcgcggcgc cggggtgggc ggggcggtgc tgcaggcgct gatcgccgag gcggcgcggc
     1441 gcgggctgaa cgaagtcgcg ctcaatgcgc agacccacgc gctcgacttc tacctgcgcc
     1501 acggcttcga gccggtgggc gaggtcttca tggaagcggg cattccccac cgcgcgatgc
     1561 ggcggccggt gtagcggccg cgcacgcgag cgcattgcgc gcggcgttca ggtcgtgccg
     1621 gcgccgaacg cgtgctcgac ccggctgcgc agttccccat ccacatccca ggtccattcg
     1681 acgaagcggg tgcggccggc gcggtcgcgc agcaccacgc tgctgcagcg cgtgccgtag
     1741 ccgggtgcgc ggatgaaggc gggcgacagc cagcgctccc actccaggct
//
LOCUS       DAOQNN010000587         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_59249_length_1788_cov_9.452972, whole genome shotgun sequence.
ACCESSION   DAOQNN010000587 DAOQNN010000000
VERSION     DAOQNN010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59249_length_1788_cov_9.452972"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKV97_17620"
     CDS             complement(<1..338)
                     /locus_tag="PKV97_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNB07758.1"
                     /translation="MSASAPARSIEFHNTTLGATIAVLQAAEPSTLADSLHKMLGGMP
                     DFFSGELLILDFGGVAQLPERIDWSGLQSLLRRYRLQPIGVMRLAAEHHEGARRAGLA
                     LVDPAQLAARP"
     gene            complement(435..1298)
                     /locus_tag="PKV97_17625"
     CDS             complement(435..1298)
                     /locus_tag="PKV97_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNB07759.1"
                     /translation="MKTPLPTSYVQRFLLEDLDIRGAVVRLTDVWEAMQDGRGYAPAV
                     ARLLGEMSAVSTVIAGNLKQPGRLTFQITGHGPVSLLVIDCAETLNLRGYAKAEGTPA
                     AGTLVELVGDGRLQLSLDIEGLDQPYQSLVPLEGDSIAEVFEHYLVQSEQQPARLWLA
                     CSAQAAVALFVQKLPGADLKDIDGWSRVQQLAHTVREDELLGLDAEQILRRLFAEEDI
                     RLFDARPVTHEWPADPDKIAEMLRALGEDEVRTVLDQHGEVVVHDDLSNHTYRFDRSD
                     VDALFRPPTLH"
     gene            complement(1295..1687)
                     /locus_tag="PKV97_17630"
     CDS             complement(1295..1687)
                     /locus_tag="PKV97_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HNB07760.1"
                     /translation="MPTTIHGIRNCDTMKKTFAWFDGAGVAYQLHDYKKAGVDAATLA
                     RWCEKLGWEALVNKRGTTWRRLAPEQQAIADTAAAIALMQAQPSLIRRPVVEFADGGL
                     VVGLDTEAFERHSRGRAQPPAGTPDTPR"
BASE COUNT          254 a          581 c          657 g          296 t
ORIGIN      
        1 gggcgggcgg cgagctgcgc cggatcgacc agtgcgagcc cggcgcgccg ggcgccctcg
       61 tggtgttccg ccgccaggcg catcacaccg atcggctgca ggcggtagcg ccgcagcagc
      121 gactgcaggc ccgaccaatc gatacgctcg ggcagttgcg ccaccccgcc gaaatcgagg
      181 atgagcagct cgccgctgaa gaagtcgggc atgccgccga gcatcttgtg cagcgaatcg
      241 gcaagcgtgc tcggttccgc cgcctgcagg acggcgatgg tggcgcccag ggtcgtgttg
      301 tggaactcga tcgatcttgc gggcgcgctg gcggacatgt gctgaacggt cttcaaaatc
      361 gagcgagtgt acttaggcga acacgttcgg gcaaggccgg cggcgcaagc cggccggcct
      421 tgccggcttc cggctcaatg cagggtgggc gggcggaaga gcgcgtccac gtcgctgcga
      481 tcgaaacggt aggtgtggtt ggacagatcg tcatgcacca ccacctcgcc atgctggtcc
      541 aggaccgtgc gtacttcgtc ctcgccgagt gcgcgcaaca tctcggcgat cttgtccgga
      601 tcggccggcc attcgtgggt gaccggacgc gcgtcgaaga ggcggatgtc ctcctcggcg
      661 aacaggcggc gcaggatctg ctcggcgtcg aggccgagca gctcgtcctc gcgcacggtg
      721 tgggccagct gctggacgcg cgaccaaccg tcgatgtcct tgaggtcggc accgggcagc
      781 ttctgcacga acagggccac cgccgcctgc gcgctgcacg ccagccacag cctggcgggc
      841 tgctgctcgg actggacgag atagtgctcg aagacctcgg cgatgctgtc gccttccagc
      901 ggcaccaggc tctggtaggg ctgatcgagg ccttcgatgt cgagggagag ttgcaggcgc
      961 ccgtcgccga cgagctcgac cagcgtgccc gcggcgggcg tgccctcggc cttggcgtaa
     1021 ccgcgcaggt tcagggtctc ggcgcagtcg atcaccagca ggctgacggg gccgtggccg
     1081 gtgatctgga aggtcagccg gccgggctgc ttgaggttgc cggcgatcac ggtggagact
     1141 gcgctcatct cgccgagcag tcgcgccacc gcgggcgcat agccgcgccc gtcctgcatg
     1201 gcttcccaga cgtcggtgag gcgcacgacc gcgccgcgga tgtcgaggtc ctcgagcagg
     1261 aaacgttgca catagctggt ggggaggggc gtcttcatct tggagtgtcc ggtgttcctg
     1321 ctggcggttg ggctcggccc cggctgtggc gctcgaaggc ctcggtatcc aggccgacga
     1381 cgagtccgcc gtcggcgaac tcgaccaccg ggcgacggat caggctgggc tgggcctgca
     1441 tcagcgcgat cgcggcggcc gtgtcggcga tcgcctgctg ctcgggcgcg agcctgcgcc
     1501 aggtggtgcc gcgcttgttg acgagcgctt cccacccgag cttctcgcac cagcgcgcga
     1561 gtgtggcggc gtcgacgccg gccttcttgt aatcgtggag ttggtaggcg acacccgcgc
     1621 cgtcgaacca ggcgaaggtc ttcttcatgg tgtcgcagtt cctgatgccg tggatcgtcg
     1681 tcggcatgac ggcctcctca gcgattgccg cgggcgcggg cgaatgcttc ggcgagtgcg
     1741 ttggcagcgg cggggcggga agcctcctgg cgcgcgccac ccttgccc
//
LOCUS       DAOQNN010000588         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_59371_length_1786_cov_8.972848, whole genome shotgun sequence.
ACCESSION   DAOQNN010000588 DAOQNN010000000
VERSION     DAOQNN010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59371_length_1786_cov_8.972848"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /gene="recA"
                     /locus_tag="PKV97_17635"
     CDS             <1..1017
                     /gene="recA"
                     /locus_tag="PKV97_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNB07761.1"
                     /translation="KAKALQAALAQIEKQFGKGSIMRLGEGEAIEDIQVVSTGSLGLD
                     IALGVGGLPRGRVIEIYGPESSGKTTLTLQVIAEMQKLAGVCAFIDAEHALDVQYAQK
                     LGVNLQDLLISQPDTGEQALEIVDALVRSGSVDLIVIDSVAALTPKAELEGEMGDALP
                     GLQARLMSQALRKLTATIKKTNCTVIFINQIRMKIGVMFGSPETTTGGNALKFYASVR
                     LDIRRIGSIKKGEEVIGSETKVKVVKNKVSPPFKTAEFDILYGEGISREGEIIDLGVT
                     AKVLDKSGAWYAYQGEKIGQGKDNCREFLRENPQLAREIENKIRESMNITLLPTAAGD
                     DASA"
     gene            1014..1520
                     /gene="recX"
                     /locus_tag="PKV97_17640"
     CDS             1014..1520
                     /gene="recX"
                     /locus_tag="PKV97_17640"
                     /inference="COORDINATES: protein motif:HMM:NF001055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HNB07762.1"
                     /translation="MSDASPPRRSAGIAGLSLKGRALRQLALRELSRQELARKLAPHA
                     ESTEQVESLLDELERLGYLSAQRVVESLLHRRAERYGNRRIRQEIESRGLDPDDHAPA
                     LEGLRDSEAQRAQALWQRRFGTHSTDPRERARQSRFLLSRGFPASLVARIVRGEPTRE
                     SSEDDDAN"
     gene            1605..>1786
                     /locus_tag="PKV97_17645"
     CDS             1605..>1786
                     /locus_tag="PKV97_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07763.1"
                     /translation="MMRCNMIQFARLSRTPRRPRSHRVRTGQRSCAGPPPLQNRLTMK
                     IHEYQGKEILRKFDVPV"
BASE COUNT          341 a          644 c          564 g          237 t
ORIGIN      
        1 aaggccaagg cccttcaggc cgcgctggcc cagatcgaaa agcagttcgg caagggctcc
       61 atcatgcgcc ttggcgaagg ggaggcgatc gaggacatcc aggtcgtttc caccggctcg
      121 ctcgggctgg acatcgccct gggcgtgggc ggcctgccgc gcggtcgcgt catcgagatc
      181 tacggtccgg aatcgtcggg caagacgacc ctgacgctgc aggtcatcgc cgagatgcag
      241 aagctcgccg gcgtgtgcgc cttcatcgac gccgaacacg cgctggacgt ccagtacgcc
      301 cagaaactcg gcgtgaacct gcaggacctg ctgatctccc agcccgacac cggcgaacag
      361 gcgctcgaaa tcgtcgacgc gctggtgcgc tccggctcgg tggacctgat cgtgatcgac
      421 tccgtcgccg cgctgacccc gaaggccgaa ctcgaaggcg aaatgggcga cgccctgccc
      481 ggcctgcagg cccgcctgat gagccaggcg ctgcgcaagc tcaccgccac gatcaagaag
      541 accaactgca ccgtcatctt catcaaccag atccggatga agatcggcgt catgttcggc
      601 agccccgaga ccaccaccgg cggcaacgcg ctgaagttct acgcctcggt gcgcctggac
      661 atccgccgca tcggcagcat caagaagggc gaagaggtca tcggcagcga gaccaaggtc
      721 aaggtcgtca agaacaaggt gtcgccaccc ttcaagaccg ccgagttcga catcctctac
      781 ggcgagggca tcagccgcga gggcgagatc atcgacctcg gcgtcaccgc caaggtgctc
      841 gacaagtccg gcgcctggta cgcctaccag ggtgagaaga tcggccaggg caaggacaac
      901 tgccgcgagt tcctccgcga gaacccgcag ctggcccgcg agatcgagaa caagatccgc
      961 gagtcgatga acatcaccct gctgcccacg gctgccggcg acgacgccag cgcttgagcg
     1021 acgcctcgcc cccgcgccgc tcggccggca tcgccggcct gagcctcaaa ggtcgggccc
     1081 tgcgacagct ggcgctgcgc gagctgtcgc gccaggaact ggcccgcaag ctggcaccac
     1141 acgccgagtc caccgagcag gtcgagtcac tgctcgatga gctggagcgg ctcggctacc
     1201 tgagcgctca acgcgtcgtc gagtccctgc tgcatcgccg cgccgagcgc tacggcaacc
     1261 gccgcatccg gcaggagatc gagtcccgcg gcctcgatcc cgacgaccac gccccggcgc
     1321 tcgagggcct gcgcgacagc gaggcccagc gcgcccaggc gctctggcag cgccgcttcg
     1381 gtacgcacag caccgacccg cgcgagcgag ctcggcaatc gcgctttctg ctcagccggg
     1441 gctttccggc ctcgctggtg gcccgcatcg tgcgcggcga accgacccgc gaatcgagcg
     1501 aagacgacga cgcgaactaa aggcgccgcc cgacggcggc gacacgcgaa atcgcatggg
     1561 cctgcgcccg attcggcgat cgagcgcatg cggacgaccg acgcttgatg cgctgcaaca
     1621 tgatacagtt cgcgcgcctt tccaggacac cgcggaggcc ccgatcgcac cgcgtccgca
     1681 ccggccaacg ctcttgcgcc gggccgcccc cacttcagaa ccgcctcacc atgaagatcc
     1741 acgagtacca aggcaaggaa atcctgcgca agttcgacgt cccggt
//
LOCUS       DAOQNN010000589         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_59559_length_1783_cov_5.734954, whole genome shotgun sequence.
ACCESSION   DAOQNN010000589 DAOQNN010000000
VERSION     DAOQNN010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59559_length_1783_cov_5.734954"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKV97_17650"
     CDS             complement(<1..243)
                     /locus_tag="PKV97_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNB07764.1"
                     /translation="MTSLQLTPAEWLLAGLLVAVLVLQAWGLLRGGGAERRLREGLGD
                     DLTDFMEQRLAARHRELLLDLHDGLAVARGQAHRRAR"
     gene            complement(248..>1783)
                     /locus_tag="PKV97_17655"
     CDS             complement(248..>1783)
                     /locus_tag="PKV97_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNB07765.1"
                     /translation="GLAGGLRELLATDLPAPSPLPLAHGLVVGMALLAGFVLPQLLRL
                     GKVSTLRVLRREFEFAEPVSGAAWALGLAALLGLIFWIAADARLGAMVAAGFAVALGV
                     FALAAWGVLRLAGRLKGQGSLRGGGWRYGIAALGRRMGSSVIQAAALGLGMTALLLLT
                     LVRADLLDNWRRMAPPDAPNRFVINIQPDQRAGIAARFAAEGLPVPPIQPMIRGRMVA
                     IEGKPVDPSAYEDERTRRLAEREFNLSYGAELPPGNVVEAGRWHGAAAQPQFSAQFSA
                     QFSVEAGLAKTFGLAVGDRVTFEIGGRRVEAPITSVRKLDWDSMRVNFFFIASPGVLE
                     DFPATLITSFHLPPARHDFTTKLVGEFPNLTVIDTAAVIAQVQSMTDKLIVIVQFVFG
                     FAVLAGLVVLYAALQSTHDERDYELAMLRTLGARNRQVRQALFAEFLVLGGVAGVLAG
                     VGASAIGWVLAEQVFRMPYVPALAPVLVGVVLGAAGVVAGGWLGTRALLSRPPLASLR
                     ALG"
BASE COUNT          289 a          674 c          633 g          187 t
ORIGIN      
        1 gcgtgctcgc cgatgcgcct ggccgcgcgc caccgcgagc ccgtcgtgca ggtcgagcag
       61 cagctcgcgg tggcgcgcgg ccaggcgctg ctccatgaag tcggtgaggt cgtcgccgag
      121 gccttcgcgc aggcggcgct cggcgccgcc gccgcgcagc agcccccagg cctgcagcac
      181 cagcaccgcc accagcaggc cggcgagcag ccactcggcc ggcgtcagtt gcaaggacgt
      241 catgcgttca gcccagtgca cgcaggctgg cgagcggcgg ccggctcagc agcgcacgcg
      301 tgcccagcca gccaccggca accacgccgg ccgcgcccag caccacgccc accagcaccg
      361 gcgccagcgc cggcacgtag ggcatgcgga agacctgctc ggccagcacc cagccgatcg
      421 cgctcgcgcc cacgcccgcg agcacgccgg cgaccccacc cagcaccagg aactcggcga
      481 acaaggcctg gcgcacctgg cggttgcgcg cgcccagggt acgcagcatg gcgagctcgt
      541 agtcgcgctc gtcgtgagtg gactgcagcg cggcatacag cacgaccagc ccggcgagca
      601 ccgcgaagcc gaacacgaac tgcacgatca cgatcagctt gtcggtcatc gactgtacct
      661 gtgcgatcac cgccgcggtg tcgatcacgg tgaggttggg gaactcgccg accagcttcg
      721 tggtgaagtc gtggcgcgcg ggcggcaggt ggaaactggt gatcagggtc gcggggaaat
      781 cctccagcac gccgggcgag gcgatgaaga agaagttcac ccgcatcgag tcccagtcga
      841 gcttgcgcac gctggtgatc ggcgcctcga cgcggcggcc gccgatctcg aacgtcacac
      901 ggtcgcccac cgccagcccg aaggtcttcg ccagcccggc ctcgacggaa aactgcgcgg
      961 aaaactgcgc ggaaaactgc ggctgcgccg ccgccccgtg ccagcgcccc gcctcgacga
     1021 cgttgcccgg cggcaactcg gcgccgtacg acagattgaa ctcgcgctcg gccaggcgcc
     1081 gcgtgcgctc gtcctcgtac gccgaggggt cgaccggctt gccctcgatc gccaccatgc
     1141 ggccgcgaat catcggctgg atgggcggca ccggcaggcc ctcggcggcg aaccgcgcgg
     1201 cgatgccggc gcgctggtcg ggctggatgt tgatcacgaa gcggttgggc gcgtccggcg
     1261 gcgccatgcg ccgccagttg tcgagcaggt cggcgcgcac cagggtgagc agcagcagcg
     1321 cggtcatgcc cagcccgagc gcggcggcct ggatcacgct gctgcccatg cggcggccga
     1381 gggcggcgat gccgtagcgc cagccaccac cgcgcaggct gccctgtccc ttgaggcggc
     1441 cggcgagcct gagcacaccc caggcggcaa gcgcgaacac gccgagcgcc accgcgaagc
     1501 cggccgccac catggcgccg aggcgcgcgt cggcggcgat ccagaagatc agcccgagca
     1561 gcgccgccag cccgagcgcc caggcggcac ccgagaccgg ctcggcgaac tcgaactcgc
     1621 ggcgcagcac gcgcagggtg ctgaccttgc ccaggcgcag cagttgcggc agcacgaagc
     1681 cggcgagcag cgccatgccc accaccaggc catgcgcgag cggcagcggc gagggcgcgg
     1741 gcaagtcggt ggcgagcagc tcgcgcagcc cgcccgccag ccc
//
LOCUS       DAOQNN010000590         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_59854_length_1778_cov_7.896692, whole genome shotgun sequence.
ACCESSION   DAOQNN010000590 DAOQNN010000000
VERSION     DAOQNN010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59854_length_1778_cov_7.896692"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="PKV97_17660"
     CDS             <1..1341
                     /locus_tag="PKV97_17660"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNB07766.1"
                     /translation="AASELSPAAERKIGEQIIREIRWRDAAYLDDAEVEEYVNRLGQR
                     LAAVSNNPGLDFDFFVVRDATLNAFALPGGFIGVHTGLILAAEGESEFASVLGHEIAH
                     VTQRHIAQIVGKQSQSAMLMIASMLVAVLAARSNSDVSTAAIAAGQAGAIQSQLGYTR
                     AFEREADRAGLETLDKAGLDVRGMPGFFERLQRNTRVYENNAPAYLRTHPLTTERIAD
                     MENRVAAMRYRQVPDSADFRFARAKLRANAGQPAEAVQELEDRLAREPKDEALAYGLA
                     RALMRAGRLDEAEARLQPLRAKAAGLAWVEGLAAEIRLARNDAGGAIRILEAAQKQFP
                     SSRSVSYALADARILGGRADVAATELRRRIDNRSGDPRLWQLLSRAYAALGQRTEQHR
                     TQAEVYYLRGSLPAAIEQLEIARRASDGDFYTLSAVDARLRELKQRLLEEKRER"
     gene            1448..1678
                     /locus_tag="PKV97_17665"
     CDS             1448..1678
                     /locus_tag="PKV97_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNB07767.1"
                     /translation="MRYDKEVDARGLNCPLPILRAKKMLSEMQSGQIVRVLATDPGSI
                     KDFQAFARQTGNELLGQGETSDKAYEFFLKRK"
BASE COUNT          286 a          631 c          615 g          246 t
ORIGIN      
        1 gccgcgtccg aactctctcc cgcggccgaa cgcaagatcg gcgaacagat catccgcgag
       61 atccgctggc gcgacgcggc ctatctcgac gatgcggagg tcgaggagta cgtgaaccgc
      121 ctcggccagc gcctcgccgc ggtaagcaac aacccggggc tggatttcga cttcttcgtc
      181 gtgcgcgacg ccacgctcaa cgccttcgcg ctgccgggcg gcttcatcgg cgtgcacacc
      241 gggctgatcc tggcggcgga gggcgagtcc gagttcgcct cggtgctcgg ccacgagatc
      301 gcccacgtca cccagcgcca catcgcgcag atcgtcggca agcagagcca gtcggcgatg
      361 ctgatgatcg cctcgatgct ggtcgcggtg ctggccgcgc gcagcaactc ggatgtcagc
      421 accgcggcga tcgctgccgg ccaggccggg gcgatccagt cccaactcgg ctacacccgc
      481 gccttcgagc gcgaggccga ccgcgccggc ctcgagaccc tggacaaggc cgggctcgac
      541 gtgcgcggca tgcccggctt cttcgagcgc ctgcaacgca acacgcgtgt gtacgagaac
      601 aacgcgccgg cttacctgcg cactcacccg ctgaccaccg agcgcatcgc cgacatggag
      661 aaccgcgtcg cggcgatgcg ctatcgccag gtgccggact cggcggattt ccgcttcgcg
      721 cgtgccaagc tgcgcgccaa cgccggccag cccgccgagg cggtgcagga gctggaggac
      781 cgcctcgcgc gcgagcccaa ggacgaggcg ctcgcctacg gtctcgcccg tgcgctgatg
      841 cgcgccggtc gcctcgacga ggccgaggcc cgcctgcagc cgctgcgcgc gaaggcggcg
      901 ggcctggcct gggtcgaggg gctggcggcg gagatccgtc tcgcgcgcaa cgacgccggg
      961 ggcgcgatcc gcatcctcga ggccgcgcag aagcagttcc cgtccagccg cagcgtgagc
     1021 tacgcgctcg ccgatgcgcg catcctcggc ggacgggcgg acgtcgccgc gaccgaactg
     1081 cgccgccgca tcgacaaccg cagcggtgat ccgcgcctgt ggcagctgct gtcgcgcgcc
     1141 tacgcggccc tcggacagcg caccgagcag caccgcaccc aggccgaggt gtactacctg
     1201 cgcggcagcc tgccggccgc gatcgagcag ctcgagatcg cgcgcagggc cagcgatggc
     1261 gacttctata ccctgtcggc ggtggatgcg cgcctgcgcg agctcaagca gcgcctgctg
     1321 gaggaaaagc gcgagcgctg aacgacctcg cgccgcatgt cgcgcagcgt gacgggcgcg
     1381 ccttccgggg cgcattgcca tcctttagaa tcggccgcct tcaacacgac ccaaccaggg
     1441 agcggggatg agatacgaca aggaagtgga tgcgcgcggg ctcaactgcc cgctaccgat
     1501 cctgcgggcc aagaagatgc tgtccgagat gcagagcgga cagatcgtcc gcgtgctggc
     1561 gaccgatccc gggtcgatca aggatttcca ggcgtttgcg cgccagaccg gcaacgagtt
     1621 gctcggccag ggtgaaacgt ccgacaaggc ctacgagttc ttcctgaagc ggaagtagtt
     1681 ttcggcgctt gccggagttc cgcgcgggct ggggcgccgc atcgcgactc gcgtcgctcc
     1741 cacgggcgct ttcggtagga gcgacgcaag tcgcgatt
//
LOCUS       DAOQNN010000591         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_60463_length_1768_cov_12.863981, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000591 DAOQNN010000000
VERSION     DAOQNN010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60463_length_1768_cov_12.863981"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1096
                     /locus_tag="PKV97_17670"
     CDS             149..1096
                     /locus_tag="PKV97_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNB07768.1"
                     /translation="MLSRTADHLYWMARYTERAENTVRMLDVSYRMSLLPGANGGDTE
                     SWSAILDISELSGAFARTGRPLSACDVIEFVVLDRDNPSSVWNCLRAARENAHAVRGT
                     LTAEMWETTNATWLEIRDMTPQKLRATDLGEFFEWVKFRSHLLRGVTFGTMLHDEAFK
                     FVRLGTFLERADNTARLLDVKYHSLLPKAEGLTSSTDFYQWGALLRSVSAFETYRHVY
                     RDVITPYKVAELLILNADMPRSLHACLDEINGILGEVASIHSAEAARQAGELHATLHY
                     GRMDDIFDAGLHPWLERFLVRINRLGDSVGENFLVPVAA"
     gene            1164..>1768
                     /locus_tag="PKV97_17675"
     CDS             1164..>1768
                     /locus_tag="PKV97_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNB07769.1"
                     /translation="MTYCVAMCLDEGLVCLSDSRTNAGVDHINTFRKMTVFERAGDRV
                     LVLMSAGNLAITQAVISLLREHAAVQDRDSLWNAPTLFECARLVGETIRGVHRQDAEA
                     LKQFGVEFNASFILAGQIGDESPRLFNIYSAGNFIEASIDTPYFQIGESKYGKPIIDR
                     IVTSKTSLDEAAKCALISMDSTIRSNLSVGLPLDLLIYRRD"
BASE COUNT          301 a          642 c          551 g          274 t
ORIGIN      
        1 gagatccgca tggtgccggg cggcctcacc cgcgtcgcgc tcaaggccgg ctcgctggtg
       61 gtcaactcct cgcagggcgg gggcaccaag gacacctggg tggtgggctg agccgcccgc
      121 cgccgcgcaa ttgccaagga gaacccccat gctgagccga accgcagacc acctgtactg
      181 gatggcgcgc tacaccgagc gcgccgagaa caccgtgcgc atgctcgacg tgtcctaccg
      241 catgtcgctg ctgcccgggg ccaacggcgg cgacaccgag agctggtccg ccatcctcga
      301 catcagcgag ctttccggcg ccttcgcgcg caccggcagg ccgctctccg cctgcgacgt
      361 gatcgagttc gtcgtgctcg atcgcgacaa cccctcgagc gtgtggaact gcctgcgcgc
      421 cgcgcgtgag aatgcgcacg cggtgcgcgg cacgctgacc gccgagatgt gggagaccac
      481 caacgcgacc tggctcgaga tccgcgacat gacgccgcag aagctgcgcg ccaccgacct
      541 gggtgaattc ttcgagtggg tgaagttccg ctcccacctg ctgcgcgggg tgaccttcgg
      601 caccatgctg cacgacgagg ccttcaagtt cgttcgcctg ggcaccttcc tcgagcgcgc
      661 cgacaacacc gcccgcctgc tcgacgtgaa gtaccacagc ctgctgccca aggccgaggg
      721 gctcaccagc tcgaccgact tctaccagtg gggcgcgctg ctgcgctcgg tgtcggcctt
      781 cgaaacctat cgccacgtct accgcgacgt gatcacgccc tacaaggtgg ccgagctgct
      841 gatcctcaac gccgacatgc cgcgctcgct gcacgcctgc ctcgacgaga tcaacggcat
      901 cctcggcgag gtcgccagca tccactcggc cgaggccgca cgccaggccg gcgagctgca
      961 cgcgacgctg cactacgggc gcatggacga catcttcgat gccggcctgc atccctggct
     1021 ggagcgcttc ctggtacgca tcaaccgcct cggcgacagc gtcggcgaga actttctggt
     1081 tccggtcgcg gcatgaccgg cgcgcagcgt ctcccggccc ggagccggac caatgaaccg
     1141 aatgaaccga atggatcgaa tccatgacct attgcgtcgc catgtgcctc gacgaggggc
     1201 tggtctgcct gtccgactcg cgcaccaacg cgggcgtgga ccacatcaac accttccgca
     1261 agatgaccgt gttcgagcgt gccggcgacc gcgtgctggt gctgatgagc gccggcaacc
     1321 tcgccatcac ccaggcggtg atcagcctgc tgcgcgagca cgccgcggtg caggaccgcg
     1381 acagcctgtg gaacgcgccg accctgttcg agtgcgcccg cctggtcggc gagaccatcc
     1441 gcggcgtgca tcgccaggac gccgaggcgc tcaagcagtt cggcgtggag ttcaacgcca
     1501 gcttcatcct cgccgggcag atcggcgacg agtcgccgcg cctgttcaac atctactcgg
     1561 ccggcaactt catcgaggcc tcgatcgaca cgccgtactt ccagatcggc gagtcgaaat
     1621 acggcaagcc gatcatcgac cgcatcgtca ccagcaagac ctcgctcgac gaggccgcca
     1681 agtgcgcgct gatctcgatg gactcgacca tccgctccaa cctgtcggtg ggcctgccgc
     1741 tcgacctgct gatctaccgc cgcgacga
//
LOCUS       DAOQNN010000592         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_60985_length_1759_cov_12.638498, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000592 DAOQNN010000000
VERSION     DAOQNN010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60985_length_1759_cov_12.638498"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1759)
                     /locus_tag="PKV97_17680"
     CDS             complement(<1..>1759)
                     /locus_tag="PKV97_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB07770.1"
                     /translation="KAQGVSEVASLGGFVREYQVTVDPLRLAGYGITLDAVTQAIRAS
                     NRDVGGRVVELAEKEYMVRGRGYLRSVDDIAGIVLKAERGTPVRVGDVARVELVPAER
                     RGIAELNGEGEVASGIVMARFGQNALDVIANVKAKIAEITPGLPAGAEIVPVYDRSEL
                     IERAIGNLKWTLLEESLIVAAVCVIFLLHVRSALVAIITLPLGILFAFIAMRSLGLGS
                     NIMSLGGIAIAIGAMVDAAIVMIENAHKHIERLPAGATPQQRAAAIVLACKEVGPALF
                     FSLLIITVSFLPVFALEGQEGRLFSPLAFTKTFAMAGAALLSVTLVPVLMLFFVRGRI
                     LPEAKNPVNRLLIWLYRPIIQGVLRFKVLTLVLAVLAMAATIVPARQIGSEFMPTLNE
                     GTLFYMPAALPGMSVTEAGRLLATTNRIIKTFPEVESVYGKAGRANTATDPAPLEMFE
                     TVINLKPQDQWRPGMTTDKLIAEMDAALKFPGLANSWTMPIKARIDMLSTGIRTPVGV
                     KVFGKDLETLEKVAQAVEAAVRKVPGASSAYAERVAGGYYVDIVPRRDQLARYGLTID
                     MVQDVIATALGGEMVTTTV"
BASE COUNT          280 a          624 c          586 g          269 t
ORIGIN      
        1 tcgaccgtgg tcgtcaccat ctcgccgccg agcgcggtgg cgatcacgtc ctgcaccatg
       61 tcgatggtga gcccgtagcg cgcgagctgg tcgcggcgcg gcacgatgtc cacgtagtag
      121 ccgccggcca cgcgctcggc gtaggcgctg ctcgcgcccg gcaccttgcg caccgccgct
      181 tccaccgcct gcgccacctt ctccagcgtc tccaggtcct tgccgaagac cttcaccccc
      241 accggggtgc ggatgccggt gctcaacatg tcgatgcggg ccttgatcgg catggtccat
      301 gagttggcca gccccgggaa cttgagcgca gcgtccatct cggcgatcag cttgtcggtg
      361 gtcatgcccg gccgccactg atcctgcggc ttcaggttga tcaccgtctc gaacatctcc
      421 agcggtgccg ggtcggtcgc ggtgttggcg cggccggcct tgccgtagac ggattccacc
      481 tcggggaagg tcttgatgat gcggttcgtc gtggcgagca ggcgcccggc ctcggtcacc
      541 gacatccccg gcagcgccgc cggcatgtag aacagcgtgc cttcgttgag cgtcggcatg
      601 aactccgagc cgatctggcg cgcgggcacg atggtcgcgg ccatcgccag cacggcgagc
      661 acgagcgtga ggaccttgaa gcgcagcacg ccctggatga tcggccggta cagccagatc
      721 agcagccggt tcaccgggtt cttcgcctcg ggcaggatgc gcccgcgcac gaagaacagc
      781 atcagcaccg gcaccagggt caccgacagc agcgcggcgc cggccatggc gaaggtcttg
      841 gtgaaggcga gcggcgagaa cagccggccc tcctggcctt cgagcgcgaa caccggcagg
      901 aaggacacgg tgatgatcag cagcgagaag aacagcgccg gcccgacctc cttgcaggcg
      961 agcacgatcg ccgccgcgcg ctgctgcgga gtggcgcccg caggcaggcg ctcgatgtgc
     1021 ttgtgcgcgt tctcgatcat cacgatcgcc gcatccacca tcgctccgat cgcgatcgcg
     1081 atgccgccca ggctcatgat gttggagccc agcccgagcg aacgcatggc gatgaaggcg
     1141 aacaggatgc ccagcggcag cgtgatgatc gccaccagtg cgctgcgaac atggagcagg
     1201 aagatcacgc acacggcggc gacgatcagg ctctcctcga gcagcgtcca cttcaggttg
     1261 ccgatcgcgc gctcgataag ctcggagcgg tcgtagaccg gcacgatctc cgcgcctgcc
     1321 ggcagcccgg gcgtgatctc ggcgatcttg gccttgacgt tggcgatcac gtcgagcgcg
     1381 ttctggccga agcgcgccat cacgatgccg gaggccacct cgccctcacc gttcaactcg
     1441 gcgatgccgc gccgctcggc cggcaccagc tcgacgcggg cgacgtcgcc cacccgcacc
     1501 ggggtgccgc gctcggcctt caacacgatg ccggcgatgt cgtccacgct gcgcaggtag
     1561 ccccggccgc gcaccatgta ctccttctcg gcgagctcca ccacgcggcc gccgacgtcg
     1621 cggttggagg cgcggatcgc ctgcgtgacc gcatcgagcg tgatgccgta gccggccagg
     1681 cgcagcggat cgaccgtgac ctggtactcg cgcacgaagc caccaaggct ggcgacctcg
     1741 gacacgccct gcgccttgg
//
LOCUS       DAOQNN010000593         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_61277_length_1754_cov_9.313125, whole genome shotgun sequence.
ACCESSION   DAOQNN010000593 DAOQNN010000000
VERSION     DAOQNN010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61277_length_1754_cov_9.313125"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PKV97_17685"
     CDS             complement(<1..348)
                     /locus_tag="PKV97_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HNB07771.1"
                     /translation="MNTSKNVLGGALLACSYSPLTGFYRTGCCETGQDDLGRHVVCVK
                     VDAAFLEFSRRAGNDLSTPRPEYRFAGLKPGDRWCLCALRWKEALEAGVAPPVILEAT
                     HEAALRVIDLETLK"
     gene            559..1173
                     /locus_tag="PKV97_17690"
     CDS             559..1173
                     /locus_tag="PKV97_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB07772.1"
                     /translation="MAGKSDSNSEFISTAQAARQLGLSLGAVQQMVESGTLAGWKTAG
                     GHRRIRQDSVDALLAHARSPSAPMRSNRERLRVMVVEDDRLLQNIYRETFLTWSLPLD
                     VHLMEHGLDALVEVGREPPDLLIADLRIPGIDGFEMIRRLRDNPLSSDIAIVVVSALG
                     PKEIAAAGGLPEDITVYRKPIPFHELHGYVQAMISMRRRARHPG"
     gene            1201..>1754
                     /locus_tag="PKV97_17695"
     CDS             1201..>1754
                     /locus_tag="PKV97_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HNB07773.1"
                     /translation="METPRAIRGMSLRAKLIAIFIAIKVVPLVLLAVFAWKAAHDLGR
                     LVTERAITMSDVMRDTQMRTGQTAIDDAIDALDDRSREAIETLTTATARSIAQFLHER
                     DGDIRAAASLPADAAAYRSFVDTRVRNIEEHGPYALDAEGERWVERDPQPRELALVRA
                     PLPDNSNSFHYRAPDTHMRGTPRPL"
BASE COUNT          279 a          664 c          552 g          259 t
ORIGIN      
        1 cttgagggtc tcgaggtcga tcacgcgcag cgccgcttcg tgggtggcct cgaggatcac
       61 cggcggcgcc acgcccgcct cgagcgcctc cttccagcgc agcgcgcaca ggcaccagcg
      121 gtcgcccggc ttcaacccgg cgaagcggta ctccggtcgc ggcgtcgaca ggtcgttgcc
      181 cgcccgtcgc gagaactcga ggaaggccgc atcgaccttc acgcagacga cgtggcgccc
      241 gaggtcgtcc tgcccggtct cgcagcatcc ggtgcggtag aaaccggtca ggggggagta
      301 gctgcaggcg agcaaggctc cgcccagcac gttcttcgag gtgttcatgg tcggcgatcc
      361 ggaagtcttt cgtagcggca tcggcgaacg cccgcggcgc ccgcggccca ccgcgtccgg
      421 atcattgtag cggcccgtcc cgtgcgcgga acacccccgc cgggtcgagg cccgcaccct
      481 tgaaattcct ttcgcatcgc cacgtcagac tgccataatt ctggtacttt taacttttcc
      541 ctcgaatcgc agacgcccat ggctggaaag tccgactcca attccgagtt catcagcaca
      601 gcccaggcag cacgccagct cggcctgtcg ctcggtgcgg tgcagcaaat ggtcgaaagc
      661 ggaacgctgg caggctggaa gaccgccggc ggccaccgcc gcatccgcca ggactctgtc
      721 gacgccctgc tcgcgcatgc gcgctcgccc tcggcaccga tgcgcagcaa ccgtgagcgc
      781 ctgcgcgtga tggtggtcga ggacgaccga ctgctgcaga acatctaccg cgagaccttc
      841 cttacctgga gcctgccgct cgacgtgcac ctgatggagc acggcctcga tgcgctcgtc
      901 gaggtcggcc gcgagccgcc cgacctgctc atcgccgacc tgcgcatccc gggcatcgac
      961 ggcttcgaga tgatccgccg cctgcgcgac aaccccctgt cgagcgacat cgccatcgtc
     1021 gtggtgtccg cgctcggtcc caaggagatc gccgcggcgg gtggactgcc ggaggacatc
     1081 accgtctatc gcaagcccat tcccttccac gagctgcacg gctacgtcca ggcgatgatc
     1141 tcgatgcgcc gccgcgctcg ccacccgggc tgagcgccgc ctgccccgcg agcctgctcg
     1201 atggagaccc ctcgcgcgat ccgcgggatg agcctgcgcg ccaagctgat cgcgatcttc
     1261 atcgcgatca aggtggtgcc gctggtgctg ctcgcggtgt tcgcctggaa ggcggcgcac
     1321 gatctgggcc ggctcgtcac cgagcgcgcg atcaccatgt ccgacgtgat gcgcgacacc
     1381 cagatgcgca ccggccagac cgcgatcgac gacgccatcg acgcactcga cgaccgctcg
     1441 cgcgaggcca tcgaaacgct caccaccgcc accgcgcgca gcatcgcgca gttcctccac
     1501 gagcgcgacg gcgacatccg cgcggccgcc agcctgcccg cggacgcggc tgcctaccgc
     1561 agcttcgtcg acacccgtgt gcgcaacatc gaggagcacg ggccctacgc gctcgacgcc
     1621 gagggcgagc gctgggtcga gcgcgacccg cagccgcgcg agctcgcgtt ggtgcgcgcg
     1681 ccgctgcccg acaactccaa cagcttccat taccgcgcac ccgacaccca catgcgcggc
     1741 accccgcgcc cgct
//
LOCUS       DAOQNN010000594         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_61904_length_1744_cov_6.164002, whole genome shotgun sequence.
ACCESSION   DAOQNN010000594 DAOQNN010000000
VERSION     DAOQNN010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61904_length_1744_cov_6.164002"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PKV97_17700"
     CDS             complement(<1..1175)
                     /locus_tag="PKV97_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB07774.1"
                     /translation="MSLSDAHTLEPNTVSAESPAAIAARLAARFAETAAARDLAGGTP
                     KAERDLLRASGLLKLSIPREYGGLGARWAETLSVVRQLARADSSVAHVFAFHHLQLAS
                     VRLFGSPEQWQPWLRDTATQGWFWGNALNPLDKRTVATRVAGGREFSGRKSFCSGALD
                     SDMLLASAVEDSAEGKLIIAVLPTRRAGITLLEDWDNMGQRQTDSGSALFERVAVAED
                     EILSRPGPLSSPNACLRPLLAQLIFVNIFLGIAEGALAEARHYTRNEARLWSASPAAH
                     VSEDPYVLAHYGEFWVALESTRALADHAAARFDAAWDRGLALSAEGRGEVALAVATAK
                     VAATRGGLDLTSRIFEVTGARSTTAALRLDRFWRNLRVHTLHDPVDYKVRELGDWAL"
     gene            complement(1269..>1744)
                     /locus_tag="PKV97_17705"
     CDS             complement(1269..>1744)
                     /locus_tag="PKV97_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB07775.1"
                     /translation="YFIDFYRQRLDIPTPRLSQEAAAELVAYHWPGNIRELENVVHFA
                     LIVCQNGVVNPGDLRLPGFALSPPGATPDPGGSHADGFATVRAGLRRLLDEDTPEVYE
                     RIEQLLFTTAFEYCRENQVRTARQLGISRNILRTQLKRFGLIGDTAADALAAADA"
BASE COUNT          251 a          532 c          687 g          274 t
ORIGIN      
        1 ttgagcgccc agtcgccgag ttcgcgcacc ttgtaatcca ccgggtcgtg cagggtatgc
       61 acgcgcaggt tgcgccagaa gcggtcgagg cgcagcgcgg cggtggtgga gcgcgcgccg
      121 gtgacctcga agatgcggct ggtgagatcc agcccgccac gggtggcggc gaccttggcg
      181 gtggcgacgg cgagcgcgac ctcgccgcgg ccttccgcgc tgagcgcgag ccctctgtcc
      241 caggccgcgt cgaaacgcgc agcggcatga tcggcgagcg cgcgggtgct ttcgagcgcg
      301 acccagaact cgccgtagtg ggcgagtaca taggggtctt cgctgacgtg ggcggcgggc
      361 gaggccgacc ataggcgcgc ctcgttgcgg gtgtagtggc gcgcttcggc gagtgcgcct
      421 tcggcgatac cgaggaagat gttcacgaag atgagctggg cgagcagtgg gcgcagacag
      481 gcgttgggcg aggacagcgg ccccggcctg gacaggatct cgtcctcggc caccgccacg
      541 cgctcgaaca gcgcactgcc gctgtcggtc tggcgctggc ccatgttgtc ccagtcctcc
      601 agcagggtga tgccggcgcg gcgggtgggg agcacggcga tgatgagctt gccctcggcg
      661 ctgtcctcga ccgccgaggc cagcagcatg tcggagtcga gcgcgccgga gcagaagctc
      721 ttgcgtccgg agaactcgcg cccgccggcc acgcgggtgg cgacggtgcg cttgtcgagc
      781 gggttgaggg cgttgcccca gaaccagcct tgcgtcgccg tgtcgcgcag ccagggctgc
      841 cactgttccg ggctgccgaa caggcgtacc gaggccagtt gcaggtgatg gaaggcgaac
      901 acatgcgcca ccgagctgtc tgcccgtgcc agctggcgta cgacggacaa cgtctcggcc
      961 catctcgcgc cgaggccgcc gtattcgcgt ggaatgctca gcttcaggag accgctcgcg
     1021 cgcagcaggt cgcgctcggc cttcggcgtg ccgccggcga ggtcgcgcgc ggcggcggtt
     1081 tcggcgaagc gtgcggccaa gcgggcggcg atggcggcgg gggattccgc gcttacggta
     1141 ttcggttcga gggtatgggc gtcgctcaga ctcatggggg atggttcctt tgccagcgga
     1201 caggtcggtt cgagcgggga ggggactgga acgggggcgg aaagcggggc ggacatgggt
     1261 gacgacattc aggcgtcggc tgcggccagg gcgtcggcgg cggtgtcgcc gatcaggccg
     1321 aagcgcttga gctgggtgcg caggatgttg cgcgagatgc cgagctgcct tgcggtgcgg
     1381 acctggttct cgcggcagta ctcgaaggcg gtcgtgaata gcagttgctc gatgcgctcg
     1441 tagacctcgg gcgtgtcttc atccagcagc cggcgcaggc ccgcgcgcac ggtggcgaag
     1501 ccgtctgcgt ggctgccgcc gggatcgggc gtcgcaccgg gaggcgacag cgcgaagccg
     1561 ggcaggcgca ggtcgccggg attgacgacg ccgttctggc acacgatcag cgcgaagtgg
     1621 acgacgttct ccagttcgcg gatgttgccc ggccagtggt aggccaccag ttccgcggca
     1681 gcttcctggc tcaggcgcgg tgtcgggatg tcgaggcgct ggcggtagaa gtcgatgaag
     1741 tact
//
LOCUS       DAOQNN010000595         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_62618_length_1733_cov_10.682956, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000595 DAOQNN010000000
VERSION     DAOQNN010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62618_length_1733_cov_10.682956"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1733)
                     /locus_tag="PKV97_17710"
     CDS             complement(<1..>1733)
                     /locus_tag="PKV97_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNB07776.1"
                     /translation="LVNPRACHETELVIEPTTAPRRIAVVGAGPAGLAFATTAAERGH
                     KVVLFEAGARIGGQFNIAMQIPGKEEFAETLRYFGRRIEQTGVALKLNTRVSAAELAG
                     KFDEVVLATGIVPRVPEIEGVDHPKVLGYLDVLRDSKPVGRRVAILGAGGIGFDVAEY
                     LSHAGISPSLAPAKFYAEWGIDARYANRGGLTRPQLETAPREIVLLQRKASKVGEGLG
                     KTTGWIHRTALKNRGVRMIAGVTYRRIDDAGLHVSIGGKDEVLAVDNVILCTGQEPQR
                     ELQAALVEAGMRVHLIGGADVAAELDAKRAIKQGIELAASIEKAASAPALLAGQLPAS
                     PGGAGIPLPQFDTLRIGLDGQVALVTLNRPDKANAMNLQMWQDLRAAMQWVDRTPAVR
                     VAVLHGAGEHFCAGIDLQMMMGILPTVKDACEARTRENLRNLILDLQDTLTSLERCRK
                     PVLAAIHGACVGGGVDLIACADLRYCAAETYFSVKEVDLGMVADVGSLQRLPRLIGEG
                     MVRELAYTGRRMDGAEAGRIGLVNRVFDTPEALMEGVMQLAQAIAAKSPLAIRGTKDM
                     LNHARDHSVADG"
BASE COUNT          221 a          623 c          618 g          271 t
ORIGIN      
        1 ccgtcggcga cgctgtggtc gcgggcgtgg ttgagcatgt ccttggtgcc gcggatcgcc
       61 agcggcgact tggcggcgat ggcctgcgcg agctgcatca ccccctccat caaggcctcg
      121 ggcgtgtcga agacccggtt cactagcccg atgcgcccgg cctccgcgcc gtccatccgg
      181 cgcccggtat aggccagctc gcgcaccatg ccctcgccga tcaggcgcgg cagacgctgc
      241 aggctgccga cgtcggcgac catgccgagg tcgacctcct tgaccgagaa gtaagtctcc
      301 gcggcgcagt agcgcaggtc ggcgcaggcg atcaggtcca cgccgccgcc gacgcaggcg
      361 ccgtggatgg cggcgagcac cggcttgcgg cagcgctcca ggctggtgag ggtgtcctgc
      421 aggtcgagga tcaggttgcg caggttctcg cgcgtgcgcg cctcgcaggc gtccttcacc
      481 gtgggcagga tgcccatcat catctgcagg tcgatgccgg cgcagaagtg ctcgccggca
      541 ccgtgcagca ccgccacgcg caccgctggc gtgcggtcca cccactgcat cgcggcgcgc
      601 aggtcctgcc acatctgcag gttcatcgcg ttggccttgt cggggcggtt cagcgtcacc
      661 agcgcgacct ggccgtcgag gccgatgcgc agggtgtcga actgcggcag ggggatgccg
      721 gcgccgccgg gagatgcggg gagttggccg gcgagcagtg caggggcgga tgccgccttc
      781 tcgatgctgg ccgccaactc gatgccctgc ttgatcgcgc gcttggcgtc gagctcggcg
      841 gccacgtccg cgccgccgat cagatgcacc cgcatgccgg cctcgaccag cgcggcctgc
      901 agctcgcgct gcggctcctg gccggtgcac aggatcacgt tgtcgaccgc cagcacctcg
      961 tccttgccgc cgatgctcac gtgcaggccg gcgtcatcga tgcggcggta ggtgacgccc
     1021 gcgatcatcc gcacgccgcg gttcttcagc gcggtgcggt ggatccagcc ggtggtcttg
     1081 cccaggccct ctccgacctt gctcgccttg cgctgcagca gcacgatctc gcgcggcgcg
     1141 gtttcgagct gggggcgggt gagcccgccg cggttcgcat agcgcgcgtc gatgccccat
     1201 tcggcgtaga acttcgccgg cgcgaggctg gggctgatcc ccgcgtggct caggtactcc
     1261 gcgacgtcga agccgatgcc gcccgcgccc aggatggcga cgcgacggcc gacgggcttg
     1321 ctgtcccgca gcacgtcgag gtagccgagc accttgggat gatccacgcc ctcgatctcc
     1381 ggcacgcgcg gcacgatgcc ggtggcgagc acgacctcgt cgaacttccc ggccagctcg
     1441 gcggccgaga cgcgggtgtt cagcttcagc gcgaccccgg tctgttcgat gcggcgcccg
     1501 aagtagcgca gggtctcggc gaactcctcc ttgcccggga tctgcatcgc gatgttgaac
     1561 tgcccgccga tgcgcgcccc ggcctcgaac agcacgacct tgtgcccgcg ctcggcggcg
     1621 gtggtggcga aggccaggcc cgccgggccg gcgccgacca ccgcgatcct gcgcggggcc
     1681 gtggtcggct cgatcaccag ctcggtctcg tggcaggcgc gcgggttcac cag
//
LOCUS       DAOQNN010000596         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_62759_length_1731_cov_6.540573, whole genome shotgun sequence.
ACCESSION   DAOQNN010000596 DAOQNN010000000
VERSION     DAOQNN010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62759_length_1731_cov_6.540573"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PKV97_17715"
     CDS             complement(<1..217)
                     /locus_tag="PKV97_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNB07777.1"
                     /translation="MSSLPRCPACNSEYTYEDGDNFVCPECAHEWPKLAAAEATEAHK
                     VWKDANGNVLQDGDSVTVIKDLKVKGSS"
     gene            complement(273..1004)
                     /locus_tag="PKV97_17720"
     CDS             complement(273..1004)
                     /locus_tag="PKV97_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNB07778.1"
                     /translation="MHKDLPDLRTPSRTPVHGLRRLVLAAAFVAGGLASHAALAATLD
                     DALSAASMGDAKELVSLIQRGLDPDTVDAQGNTLLILAAREGHTAAVEALAKNRARLD
                     YRNPAGDSALMLAVLRGHEDAARALIKAGAVVAHDGWAPLHYAAFEGRDELIADILAA
                     GADVNAPAPNKATPLMLAARNGHIDVVRRLLALPQTDLNALNDAGLSADAWALQNNNT
                     DIAELIQAERKRRGIRPPAMKITIE"
     gene            complement(985..>1731)
                     /locus_tag="PKV97_17725"
     CDS             complement(985..>1731)
                     /locus_tag="PKV97_17725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNB07779.1"
                     /translation="FPGLAEREDEILATMAANGVGTALCIGVRLEEFPRVRELAERHA
                     HLWASVGVHPDNADCEEPDVARLVALADHPRVVAIGETGLDYHWHKDAPAWQRERFRV
                     HIRAARATGKPLVVHTRNAAADTLRLMREEGAGEAGGVMHCFTESWEVAEAALELGFY
                     ISFSGIVSFRNAKELKEVARRVPLDRLLIETDSPYLAPVPHRGKTNEPGWVVHVAEEI
                     ARLRELPLAQVEQTTTDNFFRLFRHAQRPA"
BASE COUNT          234 a          556 c          657 g          284 t
ORIGIN      
        1 gggacgagcc cttgaccttg aggtccttga tcacggtgac gctgtcgccg tcctgaagca
       61 cgtttccgtt ggcgtccttc cacaccttgt gcgcctcggt ggcctcggct gcagcgagct
      121 tcggccattc gtgggcgcat tcggggcaga cgaagttgtc gccgtcctcg taggtgtatt
      181 cggaattgca ggcggggcac ctgggcaggc tgctcatttc gtttcctgat ggattggacc
      241 gcaggcggcg cgacggccgc ccggatgggg aatcattcga tcgtgatctt catcgccggc
      301 ggacggatgc cgcggcgctt gcgctcggcc tggatcagtt cggcgatgtc ggtgttgttg
      361 ttctgcagcg cccaggcgtc ggccgaaagg ccggcgtcgt tgagcgcgtt gagatcggtc
      421 tgtggcagcg cgagcaggcg gcggaccacg tcgatgtggc cgttgcgcgc cgccagcatc
      481 agcggcgtgg ccttgttggg cgcgggtgca ttgacgtcgg cgccggcggc gaggatgtcc
      541 gcgatcagct cgtcgcggcc ctcgaaggcg gcgtagtgca gcggcgccca gccgtcgtgc
      601 gcaaccacgg cgccggcctt gatgagcgcg cgtgcggcgt cctcgtggcc gcgcagcacg
      661 gccagcatca gcgcggagtc gcccgccggg ttgcggtagt cgaggcgggc gcggttcttc
      721 gccagcgcct ccacggccgc ggtgtggccc tcgcgcgcgg cgaggatcag caaggtgttg
      781 ccctgcgcgt cgacggtatc ggggtcgagg ccgcgctgga tcaggctgac gagttccttc
      841 gcgtcgccca tgctggcggc ggagagcgcg tcgtcgaggg tagcggcgag cgcggcatgc
      901 gaagccaggc cgccggcgac gaaggccgcg gcgaggacga ggcggcgcag cccatgtacc
      961 ggagtgcggg agggcgtgcg gagatcaggc aggtctttgt gcatggcgga agagacggaa
     1021 gaagttgtcg gtggtggttt gctcgacctg cgccagcggg agctcgcgca ggcgggcgat
     1081 ctcctcggcg acatggacga cccagccggg ctcgttggtc ttgccgcggt gcggcaccgg
     1141 ggcgagatag ggggaatcgg tctcgatcag caggcgatcg agcggcacgc ggcgggcaac
     1201 ctccttgagc tccttcgcgt tgcgaaagct cacgatgccc gagaacgaga tgtagaagcc
     1261 gagctcgagc gcagcctcgg ccacctccca gctctcggtg aagcagtgca tcactccgcc
     1321 ggcctcgccg gccccctcct cgcgcatcag gcgcagggtg tccgcggcgg cgttgcgggt
     1381 gtggacgacc agcggcttgc ccgtcgcacg cgccgcgcgg atgtgcaccc gaaagcgctc
     1441 acgctgccac gcgggtgcgt ccttgtgcca gtggtagtcc agcccggtct cgccgatcgc
     1501 caccacgcgc gggtggtccg cgagcgcgac gaggcgggcg acgtccggct cctcgcaatc
     1561 ggcattgtcc ggatgcacgc cgaccgaggc ccacaggtgg gcgtggcgct cggcgagctc
     1621 acgcacacgc gggaattcct ccagccgtac gccgatgcac agcgcggtgc cgacgccgtt
     1681 ggccgccatg gtggcgagga tctcgtcctc gcgctcggcg aggccgggaa a
//
LOCUS       DAOQNN010000597         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_63024_length_1727_cov_12.445574, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000597 DAOQNN010000000
VERSION     DAOQNN010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63024_length_1727_cov_12.445574"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /gene="gltA"
                     /locus_tag="PKV97_17730"
     CDS             complement(<1..929)
                     /gene="gltA"
                     /locus_tag="PKV97_17730"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNB07780.1"
                     /translation="MTPSDVKATLSFSDGSAPMELPIYKGTVGPDVIDIRKLYGQTGK
                     FTYDPGFLSTASCNSTITYIDGDKGELLYRGYPIEQLAENCDYLDTCYLLLYGDLPNA
                     DQRATFHKRVLNHTMVNEQMQFFLRGFRRDAHPMAVMTGLVGALSAFYHDSTDINNPE
                     HREIAAIRLIAKMPTLVAMAFKYSVGQPYMYPRNELSYAGNFMRMMFATPCEDYKVNP
                     VIEKALDRIFILHADHEQNASTSTVRLCGSSGTNPFAAIAAGVACLWGPAHGGANEAC
                     LNMLEDIQKMGGVEKIGEFIKQVKDKNSNVKLMG"
     gene            complement(1000..1272)
                     /locus_tag="PKV97_17735"
     CDS             complement(1000..1272)
                     /locus_tag="PKV97_17735"
                     /inference="COORDINATES: protein motif:HMM:NF015870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HNB07781.1"
                     /translation="MDTLLDAGSLSKLKWRCRRGLLENDLFIERFFANHEQSLTVRQA
                     AALEALMDLADNDLLDLLLARREPEGALAQADVLDVLAQLRQRPQR"
     gene            complement(1321..>1727)
                     /locus_tag="PKV97_17740"
     CDS             complement(1321..>1727)
                     /locus_tag="PKV97_17740"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNB07782.1"
                     /translation="IVDMTQFFKQYHSIKPYLVNDTPPPEKERLQSPEEREELNGLYE
                     CILCASCSTSCPSFWWNPDKFVGPAGLLQAYRFIADSRDQDTAARLDNLEDPYRLFRC
                     HTIMNCVDVCPKGLNPTKAIGKIKELMVRRAV"
BASE COUNT          286 a          517 c          593 g          331 t
ORIGIN      
        1 cccatcagct tgacgttgga gttcttgtcc ttcacctgct tgatgaactc gccgatcttc
       61 tccacgccgc ccatcttctg gatgtcttcc agcatgttca ggcaggcctc gttggcgcca
      121 ccgtgggccg ggccccagag gcaggccacg ccggccgcga tggcggcgaa cgggttggtg
      181 cccgacgagc cgcacagacg caccgtcgag gtcgaggcgt tctgctcgtg gtcggcgtgc
      241 aggatgaaga tgcgatccag cgccttctcg atgaccggat tgaccttgta gtcctcgcag
      301 ggcgtcgcga acatcatgcg catgaagttg ccggcgtacg acagctcatt gcgcgggtac
      361 atgtagggct ggccgacgct gtacttgaac gccatcgcca ccagcgtggg catcttggcg
      421 atcaggcgga tcgccgcgat ctcgcggtgc tcgggattgt tgatgtcggt gctgtcgtga
      481 tagaaggccg acagggcgcc gaccaggccg gtcatcaccg ccatcgggtg cgcatcacga
      541 cggaagccgc gcaggaaaaa ctgcatctgc tcgttgacca tcgtgtggtt gagcacacgc
      601 ttgtggaagg tcgcacgctg gtcggcgttg ggcaggtcgc cgtacagcag caggtagcag
      661 gtgtcgaggt agtcgcagtt ctcggccagc tgctcgatcg ggtagccgcg gtacagcagc
      721 tcgcccttgt caccgtcgat gtaggtgatc gtcgagttgc acgacgcggt ggacaggaag
      781 ccggggtcgt aggtgaactt gccggtctgg ccgtagagct tgcggatgtc gatcacgtcc
      841 gggcccacgg tgcccttgta gatcggcagt tccatcggcg ccgagccgtc ggagaacgag
      901 agggtggcct tcacgtcgga gggggtcatc attcgtgttt cctcgtagtc aaatcagcga
      961 aacggcttga agccagggat gggacacacc cggcgcgcat cacctctggg ggcgctgtcg
     1021 cagctgagcc agcacgtcca gcacatcggc ttgcgcaagg gccccctcgg gttcgcggcg
     1081 cgcaagcagc agatcgagca ggtcgttgtc ggccagatcc atcagggcct ccagggccgc
     1141 tgcctggcgc acggtcaggc tctgttcatg gttcgcgaag aagcgttcga tgaacagatc
     1201 gttctccagc aggccgcggc ggcagcgcca cttcagcttg ctgagagaac cggcgtcgag
     1261 cagagtgtcc atgcagggtc tttcgagggc ctgaccgatc acgaccgggt ccgtgcgcgg
     1321 tcagaccgcg cgacgcacca tcagctcctt gatcttgccg atggccttgg tcgggttcag
     1381 ccccttcggg cagacgtcca cgcagttcat gatggtgtgg cagcggaaca ggcggtacgg
     1441 atcctcgagg ttgtcgaggc gcgccgcggt gtcctggtca cggctgtcgg cgatgaagcg
     1501 gtaggcctgc agcaggccgg ccgggccgac gaacttgtcg gggttccacc agaagctcgg
     1561 gcagctggtc gagcagctcg cgcagaggat gcactcgtac aggccgttga gctcctcgcg
     1621 ctcctcgggc gactgcaggc gctccttctc gggcggcggc gtgtcgttga ccaggtaggg
     1681 cttgatcgag tggtactgct tgaagaactg cgtcatgtcc acgatca
//
LOCUS       DAOQNN010000598         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_63212_length_1724_cov_5.793289, whole genome shotgun sequence.
ACCESSION   DAOQNN010000598 DAOQNN010000000
VERSION     DAOQNN010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63212_length_1724_cov_5.793289"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PKV97_17745"
     CDS             <1..706
                     /locus_tag="PKV97_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB07783.1"
                     /translation="PRALEAAVAALRAAHPSSADALRLSGDLALRAKRHAEAERFYVE
                     AHARAPSQVLLARIATLAVSRGAPRAAMPQLERWLSERGEDATVREMLAELHMQAGGM
                     TAAAEQYERIIGAGEAGARVYNNLANIRLALGKGDALQAAEKAYALAPEDARVLDTLG
                     WVLAQTGRLDEALRHLRDARLRAPENGEIRWHLAEVLAKQGRSREARAELESGLQGQP
                     SGEWVEAARDLLRRLP"
     gene            837..1562
                     /locus_tag="PKV97_17750"
     CDS             837..1562
                     /locus_tag="PKV97_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB07784.1"
                     /translation="MNPSILRAALAGSLAFCSLSVSAATWTLTDSSANNPLVTGWKSG
                     SNTTDIYSTSVDFWSGNGIGIGGESSSDGQHSLDNYNGYEVALLGFNQQVRLESVKAG
                     WTQTDSDVFVMAYTGSGSASAALDGGDFKTLASRGWTLVSNLGGIGTTERSLGTTSGT
                     FANTYSSFWLIGAGNFAAGTGVNLRETTNATYDYVKIATVSGTVKTTNGGGAPVPEPG
                     TLALAGLALAGVAGLRRRRARAA"
BASE COUNT          274 a          571 c          590 g          289 t
ORIGIN      
        1 cccgcgtgcg ctggaggctg cggtcgccgc gctgcgcgcc gcccatccgt cgtccgccga
       61 cgcgctgcgg ctctcggggg atctggccct gagggcgaag cgtcatgccg aagccgagcg
      121 cttctacgtc gaggcgcatg cgcgtgcgcc ctcgcaggtg ctgctggccc ggatcgccac
      181 gctggccgtc tcgcgtggtg ccccgcgggc cgcgatgccg cagctggagc gctggctgag
      241 cgagcgtggt gaggacgcca ccgtgcgcga gatgctcgcc gagctgcaca tgcaagcggg
      301 cgggatgacg gcggcggcgg agcagtacga gcggatcatc ggcgcgggcg aggccggcgc
      361 gcgggtctac aacaacctgg ccaatatccg gctcgcgctc ggcaagggcg atgcgctgca
      421 ggccgcggaa aaggcctatg cgctcgcgcc ggaggatgcg cgcgtcctcg acacgctcgg
      481 gtgggtgctc gcgcagacgg ggcggctgga cgaggcgctc cggcacctgc gcgatgcccg
      541 cctgcgtgcg ccggaaaacg gcgagatccg ctggcatctg gccgaggtcc tcgcgaagca
      601 ggggcgatcc cgcgaagcgc gcgccgagct cgagagcgga ttgcaggggc agccgagcgg
      661 cgagtgggtc gaggccgccc gcgacctgct ccgccgcctg ccctgatgtc ggaagttttt
      721 acgcttgcct tccgctcgct ccggtgaatt ttcgcaaggc attgatttaa tgtttattca
      781 aaaaacaggc atattctttg ctgagtagag aataccgcag tgaaacgaga aaaatcatga
      841 atccatcgat ccttcgggcc gcgcttgccg gcagccttgc cttctgctcc ctttccgttt
      901 cggccgcgac ctggacgttg accgactctt cagccaacaa tccgctggtg accggctgga
      961 agtcgggtag caacaccacc gacatctaca gcacctccgt cgacttctgg agcggaaacg
     1021 ggatcggtat cggtggggag tcgtcgagtg acggacagca ctcccttgac aactacaatg
     1081 gctacgaggt cgcgctactc ggcttcaatc agcaggtgcg actcgagtcg gtcaaggcgg
     1141 gctggacgca gacggactcg gacgtgttcg tcatggcgta caccggtagc ggttccgcga
     1201 gtgcagccct tgatgggggt gatttcaaga cgctcgccag ccggggctgg acactggtca
     1261 gcaacctcgg cggtatcggc acaacggaac gcagtctcgg gaccacctcg ggcaccttcg
     1321 cgaacaccta ctccagcttc tggctgattg gcgcgggtaa tttcgctgct ggcacgggcg
     1381 taaatcttcg tgagaccacg aatgcgactt acgactacgt caagatcgcc accgtgagcg
     1441 gtaccgtgaa gaccaccaac ggtggcggcg cccccgtgcc ggagcccggc acgctcgccc
     1501 tcgccggtct ggcgctcgcc ggggtcgcgg gtttgcgcag gcgtcgcgca cgcgctgcct
     1561 gagcgcccct gttcgatcca ccggccggtc cgcatccgtg cgcgccggcc ggttcgcgtt
     1621 tacgcggcct cgccggcgcc ttcctcttgc tgctgcagca gccacatccg cgcataggcg
     1681 ccaccctgcg ccagcagcgg agcgtggcgg ccgcgctcga cgat
//
LOCUS       DAOQNN010000599         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_63614_length_1718_cov_10.903187, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000599 DAOQNN010000000
VERSION     DAOQNN010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63614_length_1718_cov_10.903187"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1718)
                     /locus_tag="PKV97_17755"
     CDS             complement(<1..>1718)
                     /locus_tag="PKV97_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HNB07785.1"
                     /translation="IIGDQEIKESLAHARPYADWLRRINIKLDTLEAPAVLDTAAADE
                     RVAPLLDRQQAFGYTQEDIKFILEPMGKAGEEGTGSMGNDSPLAVLSSKNKPLFNYFR
                     QLFAQVTNPPIDPIREQMVMSLVSFIGPRPNLLEINEINPPFRLEVSQPVLDFEGMAK
                     IRNIARYTQNKFRSAELDICYPAEWGNEGVEARLASLCADAENAVLAGTNILIVSDRK
                     LAADRVAIPALLALSAIHQHLVEKGLRTRTGLVVETGSAREVHHFAVLAGYGAEAVHP
                     YLALETLAHLADDAETAAKYVKHFVKAIGKGLMKVMSKMGISTYMSYTGAQIFEAVGL
                     KQALLDKYFTGTTSQVEGIGVFEVMEESIRLHKAAFGTDPVLHDMLDAGGEYAFRIRG
                     EEHMWTPDAIAKLQHATRSGKADTYKEYAKIINDQSRRHMTLRGLFELKAALTPVPLD
                     EVEPAKEIVKRFATGAMSLGSISTEAHTTLAVAMNRIGGKSNTGEGGEDPMRFKPLTE
                     AIKLSQVIGEKRIARDLDLNAGDSLRSAIKQVASGRFGVTTEYLVNADQIQIKMAQGA
                     KPGEGG"
BASE COUNT          238 a          537 c          622 g          321 t
ORIGIN      
        1 tggccgccct cgccgggctt ggcgccctgg gccatcttga tctggatctg gtcggcgttg
       61 accaggtact cggtggtgac gccgaagcgg cccgaggcca cctgcttgat cgccgagcgc
      121 agcgagtcgc cggcgttgag gtcgaggtcg cgcgcgatgc gcttctcgcc gatcacctgc
      181 gacagcttga tcgcctcggt cagcggcttg aaacgcatcg ggtcttcgcc gccctcgccg
      241 gtgttcgact tgccgccgat gcggttcatc gccaccgcaa gcgtggtgtg cgcctcggtg
      301 gagatcgagc cgagcgacat cgcgccggtg gcgaagcgct tgacgatctc cttggcgggc
      361 tcgacctcgt cgagcggcac cggggtgagc gcggccttga gctcgaacag gccgcgcagc
      421 gtcatgtggc ggcggctctg gtcgttgatg atcttggcgt attccttgta ggtgtcggcc
      481 ttgcccgagc gcgtggcgtg ctgcagcttg gcgatcgcgt ccggcgtcca catgtgctcc
      541 tcgccgcgga tgcggaaggc gtactcgccg ccggcgtcga gcatgtcgtg cagcaccggg
      601 tcggtgccga aggcggcctt gtgcaggcgg atggactctt ccatcacctc gaacacgccg
      661 atgccctcga cctggctggt ggtgccggtg aagtacttgt cgagcagcgc ctgcttgagg
      721 ccgacggctt cgaagatctg agcgccggtg taggacatgt aggtcgagat gcccatcttg
      781 gacatcacct tcatcaggcc cttgccgatc gccttgacga agtgcttgac gtacttggcc
      841 gcggtctcgg cgtcgtcggc caggtgggcc agggtctcga gcgcgaggta gggatgcacg
      901 gcttcggcgc cgtagccggc gagcaccgcg aagtgatgca cctcgcgcgc cgagccggtc
      961 tcgaccacca ggccggtgcg ggtgcgcagg cccttctcca ccaggtgctg gtggatcgcg
     1021 gacagcgcca gcagcgcggg gatggcgacg cggtcggcgg cgagcttgcg gtcggacacg
     1081 atgaggatgt tggtgcccgc cagcaccgcg ttctcggcgt cggcacacag cgaggccaag
     1141 cgcgcctcga cgccctcgtt gccccactcc gccgggtagc agatgtccag ctcggcggag
     1201 cggaacttgt tctgcgtgta gcgcgcgatg ttgcggatct tggccatgcc ctcgaagtcg
     1261 agcacgggct ggctcacctc gaggcggaag ggcgggttga tctcgttgat ctcgagcagg
     1321 ttggggcgcg ggccgatgaa ggacaccagc gacatcacca tctgctcgcg gatcggatcg
     1381 atcggcgggt tggtgacctg cgcgaagagc tggcggaagt agttgaacag cggcttgttc
     1441 ttcgaggaca gcaccgccag cggcgagtcg ttgcccatcg agccggtgcc ttcctcacct
     1501 gccttgccca tcggctcgag gatgaacttg atgtcctcct gcgtgtagcc gaaggcctgc
     1561 tggcgatcga gcagcggcgc gacgcgctcg tcggcggcgg cggtgtcgag cacggccggc
     1621 gcctcgaggg tgtcgagctt gatgttgatg cggcgcagcc agtcggcgta gggcctggcg
     1681 tgggcgagcg actccttgat ctcctggtcg ccgatgat
//
LOCUS       DAOQNN010000600         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_63692_length_1717_cov_8.421179, whole genome shotgun sequence.
ACCESSION   DAOQNN010000600 DAOQNN010000000
VERSION     DAOQNN010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63692_length_1717_cov_8.421179"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..523)
                     /locus_tag="PKV97_17760"
     CDS             complement(2..523)
                     /locus_tag="PKV97_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07786.1"
                     /translation="MSAVLVTAEVLARELQVSPPAVAQAKKSGRLSVIPTPDGDRFDL
                     AVAKIQWEANRKRQRSAPPAPDAPSAPPSSADGTDGGAYWEAKTRREAAEASIAELKE
                     AELRGELVRKVEVERAIASRLVALRESLEVLADRLSALVAAESDAQACRRMLRDEHRK
                     ALATFTERVLEAE"
     gene            complement(625..1377)
                     /locus_tag="PKV97_17765"
     CDS             complement(625..1377)
                     /locus_tag="PKV97_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07787.1"
                     /translation="MATVRVDTSELVKLRERFAKMREQLPFATAQALTQTAQAGQAAV
                     RQEMTRVFDRPTPYILDSTFIKPATKTNLEAHVYLKDIAGKAIAPAKAIGHEVTGGGR
                     AWKRSEGALRNAGLMGANENAVPGQAAKLDAYGNMDRGQIIQIIAYFQAFGEQGYKAN
                     TTRSKRDKLAKGTKAKGGMRYFLKRDGRGRGIYMSEQSFGRWSMRPVLMFVRRAQYQR
                     RLDMQRVVQDVARAQFGAWFADSWAQATRSAR"
     gene            complement(1377..>1717)
                     /locus_tag="PKV97_17770"
     CDS             complement(1377..>1717)
                     /locus_tag="PKV97_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07788.1"
                     /translation="RLHARTHRRISDRTMSLRHNDSKHGTPRTRGRTWQRIRLQVLAE
                     EPLCRACQAADRVTAAAEVDHIRAGMAGEPRFFAEENGHQLGTPATIEPTPNSHVFSE
                     RELDNMGRRR"
BASE COUNT          252 a          610 c          545 g          310 t
ORIGIN      
        1 cctactccgc ctcaagcacg cgttcggtga acgtcgccag cgcctttcgg tgctcgtcgc
       61 gcagcatgcg acggcaggcc tgcgcgtccg attccgccgc caccagggct gacagccgat
      121 cggcgagcac ctcgagcgac tctcgtagcg cgaccaaccg cgacgcgatc gcgcgctcga
      181 cctcgacctt gcgcaccagt tcaccacgca gctcagcctc cttgagctca gcgatcgacg
      241 cctcagcagc ctcgcggcgc gtcttcgcct cccagtaggc accgccatcg gttccatctg
      301 cgctcgacgg tggcgcagat ggtgcgtccg gagccggcgg cgccgaccgc tgacgcttgc
      361 ggttcgcctc ccattggatc ttggccaccg ccaggtcgaa tcggtcccca tcaggcgtcg
      421 ggatgacgct cagtcgaccg gacttcttcg cctgcgcgac cgccggcggg ctgacctgca
      481 gctcgcgggc gagcacttca gcggtgacga ggacggcaga catcgactct tatcgcagag
      541 gtaagggaat ggaaaagtca ggcactagcg aaagatcacg gttcgcatta cccgcacccg
      601 acgcttcgtg ggaggacccg cgagtcaccg cgcgctcctc gtcgcttgcg cccacgagtc
      661 cgcgaaccac gcgccgaact gtgcgcgcgc cacgtcctgc acgacacgct gcatatcgag
      721 ccgtcgctgg tactgggcgc gacgcacgaa catcagcaca ggtcgcatcg accatctgcc
      781 gaacgactgc tcggacatgt agatccctcg gccccggccg tcgcgcttca ggaagtagcg
      841 catgccgccc ttcgctttcg tgccctttgc gagcttgtcg cgcttggacc tggtcgtgtt
      901 cgccttgtat ccctgctcac cgaacgcctg gaagtacgcg atgatctgga tgatctgccc
      961 gcggtccatg ttcccgtagg cgtcgagctt agcggcctgg cccggcacgg cgttctcgtt
     1021 ggctcccatc aggcccgcat tgcgcaacgc gccctcgcta cgcttccaag ccctgccgcc
     1081 gcccgtgacc tcgtgcccga tcgccttcgc gggcgcaatc gctttgcctg caatgtcctt
     1141 caggtaaacg tgcgcctcga ggttcgtctt cgtcgccggc ttgatgaacg tcgaatcgag
     1201 gatgtagggc gtcgggcgat cgaacacgcg agtcatctcc tggcgcaccg cggcctggcc
     1261 ggcctgcgca gtctgggtga gagcctgcgc ggttgcgaac gggagctgct cgcgcatctt
     1321 cgcgaaccgc tcgcgcagtt tcaccagctc agacgtatcg accctgaccg tcgccatcac
     1381 cgcctccttc ccatgttgtc gagctcgcgc tcgctgaaga catggctgtt cggggtaggc
     1441 tcgatcgtcg ccggcgttcc gagctggtgc ccgttctcct cggcgaagaa gcgcggctcg
     1501 ccggccatcc cggcgcggat gtggtcgacc tcggccgccg cggtcacgcg gtccgcggcc
     1561 tggcaggcac ggcacagcgg ctcctcggcg agcacctgca gccggatgcg ctgccacgtg
     1621 cggccgcggg tgcggggcgt gccatgcttc gagtcgttgt gcctgagcga catggtgcgg
     1681 tcagagatac ggcggtgggt gcgggcgtgc aatcgag
//
LOCUS       DAOQNN010000601         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_63810_length_1715_cov_12.953614, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000601 DAOQNN010000000
VERSION     DAOQNN010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63810_length_1715_cov_12.953614"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PKV97_17775"
     CDS             <1..695
                     /locus_tag="PKV97_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNB07789.1"
                     /translation="NPSPYMYYYDMGGFQIVGASPEILVRQELRTVDGQQEKVVTIRP
                     IAGTKPRGATPELDQKNEQDLLADAKERAEHLMLIDLARNDIGRIAKTGSVKVTEAFG
                     IERYSHVMHIVSNVEGVLKDGMSSLDVLRASFPAGTLSGAPKIHAMEIIDQLEISERG
                     VYGGAVGYLSFAGDMDVAIAIRTGVIKDQTLYVQAGAGIVADSVPEMEWKETEVKMRA
                     VIRAAELVEEGF"
     gene            716..1282
                     /locus_tag="PKV97_17780"
     CDS             716..1282
                     /locus_tag="PKV97_17780"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNB07790.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEDVRVHRNDEISVRQIEALQP
                     DRLVLSPGPCSPAEAGICIAAIRHFIGKLPILGVCLGHQSIGAALGGDIVRAKVQMHG
                     KAETITTDQQGVFAGLPQQFSVIRYHSLAIARDTLPADLVVTATAADGEIMGVRHRTL
                     ALEGVQFHPESILSEHGHAMLKNFLQMR"
     gene            1316..>1715
                     /gene="trpD"
                     /locus_tag="PKV97_17785"
     CDS             1316..>1715
                     /gene="trpD"
                     /locus_tag="PKV97_17785"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254083.1"
                     /note="Catalyzes the conversion of
                     N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to
                     anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNB07791.1"
                     /translation="MSNSITNTEALTRVIEHREIFHDEMLTLMRRIMTGEMSPTVMAA
                     LMIGLRVKKETIGELTAAAQVMREFSVKVPVADRTHLVDIVGTGGDGSHTFNISTCSM
                     FVAAAAGARVSKHGGRSVSSKSGSADVLEAM"
BASE COUNT          317 a          579 c          575 g          244 t
ORIGIN      
        1 tcaacccgtc gccgtacatg tactactacg acatgggcgg cttccagatc gtcggcgcct
       61 cgccggagat cctggtgcgc caggagctgc gcaccgtcga cggccagcag gagaaggtcg
      121 tgacgatccg cccgatcgcc ggcaccaagc cgcgcggcgc cacgccggag ctcgaccaga
      181 agaacgagca ggacctgctg gccgacgcca aggagcgcgc cgagcacctg atgctgatcg
      241 acctggcgcg caacgacatc ggccgcatcg ccaagaccgg ttcggtgaag gtgacggagg
      301 ccttcggcat cgagcgctac tcgcacgtga tgcacatcgt cagcaacgtg gagggtgtgc
      361 tcaaggacgg catgagcagc ctggacgtgc tgcgcgccag cttcccggcc ggcacgctca
      421 gcggcgcgcc caagatccac gcgatggaga tcatcgacca gctggagatc agcgagcgcg
      481 gcgtctacgg cggcgcggtg ggctacctga gttttgccgg cgacatggac gtggcgattg
      541 ccatccgcac cggcgtcatc aaggaccaga cgctgtacgt gcaggcgggc gccggcatcg
      601 tggccgattc ggtgccggag atggagtgga aggagaccga ggtgaagatg cgcgcggtca
      661 tccgcgccgc cgaactggtc gaagaaggct tctgaaccgc accgggagaa ccaccatgct
      721 gctgatgatc gacaactacg acagcttcac ctacaacctg gtgcagtact tcggtgaact
      781 cggcgaggac gtccgggtgc accgcaacga cgagatctct gttcgccaga tcgaggcgct
      841 ccagcccgat cgcctcgtgc tctcgcccgg gccctgctcg ccggccgagg cgggcatctg
      901 catcgcggcg atccgccact tcatcggcaa gctgccgatc ctgggcgtgt gcctgggcca
      961 ccagagcatc ggcgcggcgt tgggcggcga catcgtgcgc gccaaggtgc agatgcacgg
     1021 caaggccgag accatcacca ccgaccagca gggcgtgttc gccgggctgc cgcagcagtt
     1081 cagcgtgatc cgctaccact cgctggcgat tgcgcgcgac acgctgccgg ccgacctggt
     1141 ggtcaccgcc accgcggccg acggcgagat catgggcgtg cgccaccgca cgctggcgct
     1201 ggagggggtg cagttccacc ccgagtccat cctcagcgag cacggccacg cgatgctgaa
     1261 gaacttcctg caaatgcgct gaacgcacgc gctgaacgga accccggaga cgcccatgtc
     1321 caactcgatc accaacaccg aagccctgac gcgcgtcatc gagcaccgcg agatcttcca
     1381 cgacgagatg ctcacgctga tgcgacgcat catgaccggc gagatgtcgc ccaccgtgat
     1441 ggccgcgctg atgatcggcc tgcgggtgaa gaaggaaacc atcggcgagc tgaccgccgc
     1501 cgcgcaggtg atgcgcgagt tctcggtcaa ggtgccggtg gccgaccgca cccacctggt
     1561 cgacatcgtc ggcaccggtg gcgacgggtc gcacaccttc aacatctcca cctgcagcat
     1621 gttcgtggcc gccgccgcgg gcgcgcgggt gagcaagcac ggcgggcgca gcgtctccag
     1681 caagtccggc agtgccgacg tgctggaggc catgg
//
LOCUS       DAOQNN010000602         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_64223_length_1709_cov_12.330713, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000602 DAOQNN010000000
VERSION     DAOQNN010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64223_length_1709_cov_12.330713"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..>1709
                     /locus_tag="PKV97_17790"
     CDS             315..>1709
                     /locus_tag="PKV97_17790"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNB07792.1"
                     /translation="MPACARPGVYVPRQTHDCDAAVLVRRHLPGFLARLEEAGHALPG
                     FVKAELEGFATCGDFERGFVRTACRTCGDELRVPFSCKGRGFCPSCMGRRMAEGAALL
                     VDHVLPAVGYRQWVLSFTGRMAVRLGYDQVLLAKVAEAFARAVMHDMRWSVKERHGLG
                     SVEPLHAGVFTVVQRFRSDLGLYVHLHSLVTDGAFEEAGADVRFLPARAPTPERMTAV
                     LAQVHKALAAVAEDDDLDMDPALAVCVQLALAGPHAVPPPEPAARPSLTVSAFDMHLH
                     AATTVDGRDRKQLERVCRYMLRPPFAHDAVKALPDRRVRVLFKAPWRSGVAHADMTPD
                     KFIARLCALVPPPGFHMVRYFGVLASHHHLRARIVPPAAVPAPVLQLALGLTGAANDA
                     SGEPADALPRPRRLAWASLLARVFAIDVTVCRKCGGRMRILEVVSDPDDIARVLHGAR
                     APPPPPRPHPPGQLL"
BASE COUNT          201 a          664 c          588 g          256 t
ORIGIN      
        1 cggtgctggc ggtagctcgg accgtcgatg tcgagggcat aggtgacggt gacattcccg
       61 cgccccaggc caccgtccct tggcgcccca tccgagcgaa atcgctcatt cggctgtcac
      121 cccaggctgt caccccaggt cggtggcgta acaggctcag gcgagccgta gcggccccgt
      181 agcagtgtcc cgtgggccgg ccaccccggc ccgcccgcgg gccagggcgc cgtcgtgcgc
      241 ggcgcgagtt cggcagcaac tcgccgcctt gccgagcgac tacgcgcggc ggggcggatg
      301 cagcgcagcg cggcatgccc gcatgcgcgc gcccgggcgt ctacgtcccg cggcagaccc
      361 acgactgcga cgccgcggtg ctcgtgcgcc ggcatctgcc gggcttcctc gcgcggctcg
      421 aggaggccgg ccacgccctg ccggggttcg tgaaggcgga gctcgagggt ttcgcgacct
      481 gcggcgactt cgagaggggc ttcgtgcgga cggcgtgccg cacctgcggc gacgagctcc
      541 gggtgccctt ttcgtgcaaa ggccgcggct tctgtccgtc gtgcatgggc cggcgaatgg
      601 cggagggcgc ggcgctgctg gtggaccatg tgttgcccgc ggtcggctat cgccagtggg
      661 tcctctcgtt cacggggcgc atggcggtgc ggctgggtta cgaccaggtg ctgctggcga
      721 aggtcgccga ggccttcgcg cgggcggtga tgcacgacat gcgctggtcg gtgaaggagc
      781 gccacggcct cgggtcggtg gagccgctgc acgccggggt gttcacggtg gtgcagcgct
      841 tccgcagcga cctcggcctc tacgtacatc tccacagcct cgtcaccgac ggcgccttcg
      901 aggaggccgg cgccgacgtg cgcttcctgc ccgccagggc gccgacaccc gagcgcatga
      961 ccgccgtgct cgcgcaggtc cacaaggccc tcgccgccgt cgccgaggat gatgacctcg
     1021 acatggaccc ggccctcgcc gtctgcgtgc agctcgcgct cgccggcccg cacgcggtcc
     1081 cacctcccga gcccgccgcc cggccgtcgc tgacggtctc cgcgttcgac atgcacctgc
     1141 acgccgcgac caccgtcgac ggtcgcgacc gcaagcaact cgagcgagtg tgccgctaca
     1201 tgttgcggcc gcccttcgcc cacgacgccg tcaaggcgct ccctgaccgc cgggtccgcg
     1261 tcctcttcaa ggccccgtgg cgcagcggcg tcgcccacgc cgacatgacc ccggacaagt
     1321 tcatcgccag actctgcgcg ctcgtgcccc cgccgggttt ccacatggtc aggtacttcg
     1381 gggtgctagc gagtcaccat cacctgcgcg cccgcatcgt tccgcccgcc gccgtgccgg
     1441 cgcccgtgct ccagctcgcg cttggcctca ccggcgccgc gaacgacgcc tccggcgagc
     1501 cggccgacgc gttgccgagg ccgcgccgcc tcgcgtgggc gagcctcctc gcgcgggtct
     1561 ttgccatcga cgtcaccgtc tgccgcaagt gcggcgggcg catgcgcatc ctcgaagtcg
     1621 tctccgaccc cgacgacatc gcccgcgtgc tccacggtgc tcgcgctccg ccgccaccac
     1681 cgcggcccca tcctccgggc cagctcctc
//
LOCUS       DAOQNN010000603         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_64229_length_1709_cov_5.111850, whole genome shotgun sequence.
ACCESSION   DAOQNN010000603 DAOQNN010000000
VERSION     DAOQNN010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64229_length_1709_cov_5.111850"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PKV97_17795"
     CDS             <1..832
                     /locus_tag="PKV97_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNB07793.1"
                     /translation="FKIDPCPPADPQKKGIVEAGVKYVKGNFLPLREFRDLVDLNAQA
                     RAWVMTEAGVRIHGTTRRQPLDLFEIERPLMRALPAVAPDLGSWHRVVVHRDCFVSHA
                     RILYSAPYPLVGKTLWLRATDGAVSIYEDYRLVAMHRRGRRPGERIWILEHLPPDARA
                     FLAHDHDWCLEQARRVGPGCAELIERLLADRILERLRSAQGVLGLRETYGAPRLEAAC
                     LRALYHASPFYRTVKTILACGLDHTEAPAPASPHVHAPEARFARNAQSLFDPDASARH
                     "
     gene            851..1639
                     /gene="istB"
                     /locus_tag="PKV97_17800"
     CDS             851..1639
                     /gene="istB"
                     /locus_tag="PKV97_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004349242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNB07794.1"
                     /translation="MNPIPALTPHLKQLRLSGILDSLEARNRQAIDAKLAYTEFLGLL
                     IGDEIARREHKKFDMRLRRAAFRTTKTLDAFEFERLPQLNRALVHDLATGRYIEEHAP
                     VLIVGPCGTGKSHLAQALGHAAVRQGVDVAFTSCAHLTTSLNAARAVGNYERKLAALA
                     KVPLLIVDDFGLKPLRPPADEDLHDLIAERYETAATIVTSNLDFPEWDQAFPANRVLA
                     SATLDRLRHNAYCLVLDGQSYRAPKQPPGAVKPSIAKTSKTTHP"
BASE COUNT          267 a          687 c          506 g          249 t
ORIGIN      
        1 cttcaagatc gatccgtgcc cgccggccga cccgcagaag aagggcatcg tcgaggccgg
       61 ggtgaagtac gtcaagggca acttcttgcc gctgcgcgag ttccgcgacc tcgtcgactt
      121 gaacgcccag gcgcgcgcct gggtgatgac cgaggcgggc gtgcgcatcc acggcaccac
      181 gcggcgtcaa ccgctggatc tgttcgaaat cgagcgcccg ctgatgcgcg cgctgccggc
      241 ggttgctccg gatctgggca gttggcatcg cgtggtcgtg catcgcgact gcttcgtctc
      301 ccacgcccgc atcctgtact cggcacccta cccgctggtg ggcaagacgc tgtggctgcg
      361 cgccaccgac ggcgcggtct cgatctacga ggactaccgc ctggtggcga tgcatcggcg
      421 gggccgacgc cccggcgagc gcatctggat cctcgagcac ctgccgccgg acgcccgcgc
      481 gttcctcgcc cacgaccatg actggtgcct ggagcaggcg cgtcgcgtcg gccccggctg
      541 cgccgagctc atcgagcggc tgctcgccga tcgcatcctc gagcggctgc gctcggccca
      601 gggtgtgctg ggcctgcgcg agacctacgg cgcgccgcgc ctggaggccg catgcctgcg
      661 cgccctgtac cacgccagcc ccttctaccg caccgtcaag acgatcctgg cctgcggcct
      721 ggaccatacc gaggcgcccg cgccggcctc gccccacgtg cacgcgcccg aggctcgctt
      781 cgcgcgcaac gcccagtccc tgttcgaccc cgacgccagc gcgcgtcact gatccacgga
      841 gcccgtcccc atgaacccga tccccgcgct caccccgcac ctgaagcagc ttcgcctgtc
      901 gggcatcctc gactcgctcg aggcccgcaa ccgtcaagcc atcgacgcca agctcgccta
      961 caccgagttc ctcggcctgc tcatcggcga cgagatcgcc cggcgcgagc acaagaagtt
     1021 cgacatgcgg ctgcgccgcg ccgcgttccg taccaccaag acgctggacg ccttcgagtt
     1081 cgagcggctg ccgcaactga accgcgcgct cgtccacgat ctggccaccg ggcgctacat
     1141 cgaggagcac gccccggtgc tcatcgtggg tccgtgcggc accggaaaaa gccatctcgc
     1201 gcaggccctc ggccacgccg ccgtgcgcca gggcgtcgac gtcgccttta cctcctgtgc
     1261 gcacctcacc acgagcctga acgccgcgcg cgccgtcggc aactacgagc gcaagctcgc
     1321 cgcgctcgcc aaggtcccac tgctcatcgt cgacgacttc ggcctgaagc cgctgcgccc
     1381 gcccgccgac gaggatctgc acgacctcat cgccgaacgc tacgagaccg ccgccaccat
     1441 cgtcaccagc aacctcgact tccccgagtg ggaccaggcc ttccccgcca accgcgtact
     1501 cgcctcggcc acgctggatc ggctgcgcca caacgcctac tgcctcgtgc tcgacggcca
     1561 gtcctaccgc gccccgaagc agccgcccgg cgccgtcaaa cccagcattg caaagacctc
     1621 gaaaaccacc catccttgac acccgccagg atgtcgccaa accccactcg cactggctcc
     1681 aatacgccgg tcatccctgg cttacatgg
//
LOCUS       DAOQNN010000604         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_64430_length_1706_cov_11.996366, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000604 DAOQNN010000000
VERSION     DAOQNN010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64430_length_1706_cov_11.996366"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /gene="rplB"
                     /locus_tag="PKV97_17805"
     CDS             <1..624
                     /gene="rplB"
                     /locus_tag="PKV97_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNB07795.1"
                     /translation="RNKDGIPAKVERIEYDPNRSAHIALLCYADGERRYIIAPRGVEV
                     GQQLISGAEAPIKAGNALPIRNIPVGSTIHCIEMVPGKGAQLARAAGTSVQLLAREGS
                     YAQLRLRSGEIRRVHVECRATIGEVGNGEHGLRKIGKAGANRWRGIRPTVRGVAMNPV
                     DHPHGGGEGRTGEAREPVSPWGTPAKGYRTRSNKRTDGMIVQRRHKR"
     gene            635..910
                     /gene="rpsS"
                     /locus_tag="PKV97_17810"
     CDS             635..910
                     /gene="rpsS"
                     /locus_tag="PKV97_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNB07796.1"
                     /translation="MARSIKKGPFIDAHLLKKVDAARTSNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGKQHIPVFVTENMVGHKLGEFALTRTFKGHAASKKAKR"
     gene            920..1249
                     /gene="rplV"
                     /locus_tag="PKV97_17815"
     CDS             920..1249
                     /gene="rplV"
                     /locus_tag="PKV97_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNB07797.1"
                     /translation="METKAILRGVRLSAQKGRLVADLVRGMPVGRALDTLAFSPKKGA
                     KIIRKVLESAIANAEHNDGADIDALKVKTIHVEEGMTLKRFTARAKGRGNRILKPTCH
                     VYLTVGE"
     gene            1259..>1706
                     /gene="rpsC"
                     /locus_tag="PKV97_17820"
     CDS             1259..>1706
                     /gene="rpsC"
                     /locus_tag="PKV97_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNB07798.1"
                     /translation="MGQKIHPTGFRLAVTRDWGSRWFANSKDYAGMLHEDLKVRDYLK
                     KRLAHASVGRVVIERPAKNARVTLYSARPGVVIGKKGEDIEALKRDLQKIVGVPVHVN
                     IEEVRKPELDAKLIADSIAQQLEKRIMFRRAMKRAMQNAMRLGAQGI"
BASE COUNT          342 a          558 c          546 g          260 t
ORIGIN      
        1 cgcaacaagg acggcattcc ggccaaggtc gagcgcatcg agtacgaccc gaaccgctcc
       61 gcgcacatcg cgctgctgtg ctatgccgat ggcgagcgcc gctacatcat cgcgccgcgc
      121 ggcgtagagg tcggccagca actgatcagt ggcgcggaag cgccgatcaa ggcgggcaac
      181 gccctgccga tccgcaacat cccggtcggc tcgacgatcc actgcatcga gatggttccc
      241 ggcaagggtg cgcaactcgc gcgcgccgcc ggcacttcgg tgcagctgct cgcccgcgaa
      301 ggctcgtacg cccagctccg cctgcgctcc ggcgagatcc gccgtgtgca cgtcgagtgc
      361 cgcgccacca tcggtgaagt cggtaacggc gagcatggcc tgcgcaagat cggcaaggct
      421 ggcgcgaacc gctggcgcgg tatccgtccg accgttcgcg gtgtggcgat gaacccggtg
      481 gatcacccgc atggtggtgg cgaaggccgc accggcgagg cgcgcgaacc ggtcagtccg
      541 tggggcacgc cggccaaggg ctatcggacg cgttcgaaca agcgcaccga cggcatgatc
      601 gtgcagcgtc gtcacaagcg ttaaggggta agagatggca cgttctatca agaaaggccc
      661 gttcatcgac gcgcacctgc tcaagaaggt cgacgcggct cggacgagca acgacaagcg
      721 cccgatcaag acctggtcgc gccgctccac gatcctcccc gatttcatcg gcctgacgat
      781 cgcggtgcat aacggcaagc agcacattcc tgtattcgtg accgagaaca tggtcgggca
      841 caagctgggc gaattcgcgc tgacgcggac cttcaagggt cacgccgcca gcaagaaggc
      901 caagcgttaa ggggacgaca tggaaaccaa agctattctc cgtggcgttc ggctgtcggc
      961 ccagaagggc cgtctggtgg ccgacctggt ccgcggcatg cccgtcggcc gcgcgctcga
     1021 caccctggcc ttctctccca agaagggcgc gaagatcatc cgcaaggtgc tcgagtcggc
     1081 gatcgccaac gccgagcaca acgacggtgc cgatatcgac gcgctgaagg tcaagaccat
     1141 tcacgtggaa gagggtatga cgctcaagcg cttcaccgcg cgtgcgaagg gtcgtggcaa
     1201 ccgcatcctg aagcccacct gccacgtcta cctgaccgtt ggcgagtaag gagtaaacat
     1261 gggtcagaaa atccatccta ccgggttccg cctcgccgtc acgcgtgact ggggctcgcg
     1321 ctggttcgcc aacagcaagg actacgccgg catgctgcac gaggacctca aggtccgcga
     1381 ttacctgaag aagcgcctcg cgcacgcttc ggtgggtcgc gtggtcatcg agcgtcccgc
     1441 caagaacgcc cgcgtaaccc tgtacagcgc ccgcccgggc gtcgtcattg gcaagaaggg
     1501 cgaggacatc gaggcgctca agcgtgatct gcagaagatc gttggcgttc cggtccacgt
     1561 caatatcgag gaagtgcgca agcccgaact cgatgccaag ctgatcgccg actcgatcgc
     1621 ccagcagctc gagaagcgca tcatgttccg tcgcgccatg aagcgtgcga tgcagaacgc
     1681 catgcgtctc ggtgcccagg gcatca
//
LOCUS       DAOQNN010000605         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_64706_length_1702_cov_13.004857, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000605 DAOQNN010000000
VERSION     DAOQNN010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64706_length_1702_cov_13.004857"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PKV97_17825"
     CDS             <1..697
                     /locus_tag="PKV97_17825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB07799.1"
                     /translation="EQPSEFSEFGVRYRFQARHGGQDPWLFLDLRAARRRVMQEAAGK
                     SLLNVFAYTCGVGIAAARAGARHVVNVDFAESALAVGKDNARLNELPIRVRFIKSDAF
                     AALRQYAGIGQPKMVRGKHLPPFPELAPHRFDLVFLDPPRYAKSPFGVVDLVHDYAAL
                     FKPALLATEEGGTLICCNNVARVDREDWLEQLERSARKAGRAVREAEWITPEADFPSR
                     DDNPPLKVVLLRV"
     gene            754..1098
                     /locus_tag="PKV97_17830"
     CDS             754..1098
                     /locus_tag="PKV97_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07800.1"
                     /translation="MAGFENYQQEIRRIEDEIEHMGVALGIDWSDEAQVRALAREALD
                     HSQDRIREAAASPDDHELGAKVTLFGLASLMLRTMEESAGVGIESHGGPVWKAFGRAL
                     WLEAEQRRQPEA"
     gene            1279..>1702
                     /locus_tag="PKV97_17835"
     CDS             1279..>1702
                     /locus_tag="PKV97_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNB07801.1"
                     /translation="MLQKFPAFAGVQGPVVVIVMDGYGIPRHEVGSAIDAARKPTLDR
                     LFAQYPNIVLRAHGTAVGMPSDDDMGNSEVGHNAIGAGQVYAQGAALVANAIAEGAIW
                     QGEAWQQIVAGAKTGASGKAGVLHFIGLFSDGNVHSHID"
BASE COUNT          282 a          614 c          570 g          236 t
ORIGIN      
        1 cgagcagcct tcggagtttt ccgagttcgg cgtgcgctac cgcttccagg cccgccacgg
       61 cgggcaggac ccctggctgt tcctcgacct gcgcgccgcg cgccgccgcg tcatgcagga
      121 ggccgcgggc aagtccctgc tcaacgtgtt cgcgtacacc tgcggcgtcg gcatcgccgc
      181 agccagggcc ggcgcgcgcc acgtggtgaa tgtggacttc gccgagtccg ccctcgcggt
      241 cggcaaggac aacgcacgcc tcaacgagct gccgatccgg gtgcgcttca tcaagtccga
      301 cgccttcgcc gcgctgcgcc agtacgccgg catcggccag ccgaagatgg tgcgcggcaa
      361 gcacctgccg cccttccccg agctcgcgcc gcaccgcttc gacctcgtct tcctcgatcc
      421 cccgcgctac gccaagagcc ccttcggcgt ggtcgacctg gtgcacgact acgccgcgct
      481 gttcaagccc gcgctgctcg ccacggaaga gggcggcacc ctgatctgct gcaataacgt
      541 cgcccgcgtg gatcgcgagg actggctcga gcagctcgag cgcagcgccc gcaaggcggg
      601 ccgtgcggtg cgtgaggccg aatggatcac ccccgaggcg gactttccca gccgcgacga
      661 caacccgccg ctgaaggtgg tgctgctgcg cgtttgagca aggcgccggc acgccgccac
      721 cgcccatcca ccggacacgc cccaggagag gtcatggcag gattcgagaa ctatcagcaa
      781 gagatccgca ggatcgagga cgagatcgag cacatgggcg tcgcgctcgg catcgactgg
      841 tccgacgagg cccaggtgag ggcgctcgcg cgcgaggcgc tggaccactc ccaggacagg
      901 atccgcgagg ccgcggccag cccggacgac cacgagctgg gggcgaaggt gacgctcttc
      961 ggcctcgcca gcctgatgct gcgcacgatg gaagagagcg ccggcgtcgg catcgagagc
     1021 catggcggac cggtctggaa ggccttcggc cgcgcgctgt ggctcgaggc cgagcagcgc
     1081 cgccagcccg aagcctgagc cttgtggatc ccgatcgcgc cgcatgcggt ctcggcgcgg
     1141 gtggtgcgcg gaaaaagtca ttgggccgat aaacaacgcg catgaaaatc cgccgcggat
     1201 ttgacggaaa atatggcttt cgccggtccg cagccccgct gccccgccgg ccccggattt
     1261 ctccttcagg aagcgaccat gctgcagaaa ttccccgcct tcgccggcgt ccagggccct
     1321 gtcgtcgtca tcgtgatgga cggctacggc attcccaggc acgaggtcgg cagcgccatc
     1381 gacgccgcgc gcaagcccac gctcgaccgc ctcttcgccc agtaccccaa catcgtgctg
     1441 cgcgcccacg gcaccgcggt gggcatgccg tcggacgacg acatgggcaa ctccgaggtc
     1501 ggccacaacg ccatcggcgc cggccaggtg tatgcgcagg gcgcggcact ggtcgccaac
     1561 gcgatcgccg aaggcgcgat ctggcagggc gaggcctggc agcagatcgt cgccggcgcc
     1621 aagacgggag ccagcggaaa ggcaggggtg ctccacttca tcggcctgtt ctccgacggc
     1681 aacgtgcaca gccacatcga cc
//
LOCUS       DAOQNN010000606         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_64904_length_1699_cov_7.830900, whole genome shotgun sequence.
ACCESSION   DAOQNN010000606 DAOQNN010000000
VERSION     DAOQNN010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64904_length_1699_cov_7.830900"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PKV97_17840"
     CDS             <1..632
                     /locus_tag="PKV97_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07802.1"
                     /translation="DGSNWGARVSRLVDGWDLSAFYYRSYDVSPTLYALNQGVARLRH
                     DRIGQLGGTFSKDLGSFVLKGEAVHTHGRSLNTFSSGPGLSIGLLPTDMVDYALGVDV
                     PAGDWRFNVQYYGRWLEEHVPALMADRHEQGVTLQVVHGAGTNLEAEVLALSSINRSD
                     HLIRPKLTWKFAPAWRLVGGVDVFGGNGRGFFSRYDRNDRVYLELRHMF"
     gene            complement(648..1445)
                     /locus_tag="PKV97_17845"
     CDS             complement(648..1445)
                     /locus_tag="PKV97_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07803.1"
                     /translation="MRQIHSSRGTNCPPPVRDLAYLALSGAGFQSPETSPHQWFDENT
                     AALRGLSFERSGFQVCIANGSEALKRQTAKLIERMYSARGLFPYGMHANIDPRDITVV
                     ALLDGEAVATATIRVDHGGGLLADTLYSKEIDHVRATGGRVCEITQLAIDPERGSHEA
                     LAGVMQAVFVITQATRRVTDIFAEVNPRHAGFHQRVFGFRRVGVEKICPRVNAPAVLL
                     HLPLSEFEERVARHERLMHMNGDRVHRLLPDPVQSRQLIQAMTGKAA"
BASE COUNT          283 a          513 c          575 g          328 t
ORIGIN      
        1 tggatggctc gaactggggt gcgcgcgtct cgcgactcgt cgatgggtgg gatctcagcg
       61 ccttctatta ccgcagctac gatgtctcgc cgacgctata cgccctgaac cagggtgtgg
      121 cccgcctgcg acacgaccgg atcgggcagc ttggcggcac tttcagcaag gatctcggca
      181 gcttcgtgct gaagggcgag gcggtccata cgcacggtcg cagcctgaat acgttctcga
      241 gcggtccggg cctctccatc gggctgctgc cgaccgacat ggtcgactat gcgctcgggg
      301 tcgatgtgcc cgcgggcgac tggcgcttca acgtgcagta ctacgggcgc tggctggagg
      361 agcatgttcc ggccttgatg gccgatcggc acgagcaggg cgtcaccttg caggtcgttc
      421 atggcgcggg cacgaacctg gaggcggaag tcctcgcgct gtccagcatt aaccgctcgg
      481 atcacctgat tcgaccgaag ctgacctgga aattcgcccc ggcatggagg ctcgtcggtg
      541 gcgtcgacgt cttcggcggg aatggaaggg ggtttttcag ccgttacgat cggaacgatc
      601 gtgtctatct cgaactgcgc cacatgttct gagtgccccg ggcgggctca ggccgcctta
      661 ccggtcatcg cctggatcaa ctgcctggac tggacgggat ccggcagcag gcgatgaacc
      721 cgatcgccgt tcatgtgcat caggcgttcg tggcgggcga cgcgctcctc gaactcggac
      781 aggggcagat ggaggagcac ggccggagcg ttgaccctgg ggcagatctt ctccacgccc
      841 acgcgacgaa agccgaatac gcgctgatgg aatcccgcat ggcgcggatt gacctcggcg
      901 aaaatgtcgg tgacgcgccg cgtcgcctgg gtgatcacga acacggcctg catcacgcca
      961 gccagcgctt cgtgcgatcc gcgctcggga tcgatcgcca actgtgtaat ttcgcacact
     1021 cgacccccgg tcgcgcgcac gtggtcgatc tctttgctgt acaaagtgtc cgcgagcaat
     1081 ccaccaccgt gatcgacgcg gatcgtcgcc gtcgcgaccg cttccccgtc gaggagagcg
     1141 accacggtga tgtcgcgcgg gtcgatgttg gcgtgcatcc cgtagggaaa aaggccgcgc
     1201 gccgagtaca tgcgctcgat cagcttggcg gtctggcgct tgagcgcttc actgccgttg
     1261 gcgatgcaga cctggaagcc gctgcgttcg aacgaaaggc cgcgcagtgc ggcggtgttc
     1321 tcgtcgaacc actgatgcgg cgacgtctcc ggcgattgga atccggcgcc agacaacgct
     1381 aggtaggcga ggtcgcgtac gggcgggggg cagtttgtgc cccgcgaaga gtggatctgg
     1441 cgcattggag tggcccaatt cgtttacgtt ctgggtggtc tggagtgcgc gcgcggcgag
     1501 cgcggacgac tcccatcgga tcaatgataa tccggaacgg gcgagccgtg aatctagccg
     1561 acttcatgtc tcctgaaatg taggcgcttg tgtgcaatcc tgcctggcgt cgcggcgggc
     1621 ggtgcccgta cgatggccct cgcattcgcg tcgtcgtata tcattcgccg tccgggactt
     1681 acgaaggggc ttgtcatgt
//
LOCUS       DAOQNN010000607         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_65677_length_1688_cov_9.314758, whole genome shotgun sequence.
ACCESSION   DAOQNN010000607 DAOQNN010000000
VERSION     DAOQNN010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65677_length_1688_cov_9.314758"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="pyk"
                     /locus_tag="PKV97_17850"
     CDS             complement(<1..890)
                     /gene="pyk"
                     /locus_tag="PKV97_17850"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNB07804.1"
                     /translation="MSRHTKIVATLGPASSDLLTLERMVHAGLDVVRMNFSHGKPEDH
                     VARAETIRDASARAGRPIGILADLQGPKIRVGKFEEGRVTLTRDAPFILDARCELGNV
                     QRVGLDYKDLPKDVKPGDVLLLDDGRLKLTVQRVLGHEIHTTVKVGGELSNNKGINRQ
                     GGGLTAPALTAKDMDDIRTAALIGVDFVAVSFPKSAADMYMARQLMRAAGCSALLIAK
                     IERTEAVANLDEILDASDGIMVARGDLAVEVGDAAVPALQKKMIRAARDRNKLTITAT
                     QMMESMITSPVPTRAEVSDVA"
     gene            1068..>1688
                     /locus_tag="PKV97_17855"
     CDS             1068..>1688
                     /locus_tag="PKV97_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNB07805.1"
                     /translation="MALPGKPSRRTAAMAVSSVVLCMLSASAAAVPSSLYVFGDSLSD
                     NGNLYAWTSNPSNPVTFGLPIPVSPPYEQGTFTNGPVYAERLWDRLGLPGELAPAALP
                     GGTNHAVGGARSRYHSFDVGPDGLPPLVWPANFASFSLTGQFQGYRDGLAGGAADASA
                     LYLVWAGANDLQDVLRLAQLSGPAAAGARLAEAVGDVAGVIGGLVGL"
BASE COUNT          211 a          559 c          630 g          288 t
ORIGIN      
        1 gccacgtcgg agacctcggc gcgggtgggc accgggctgg tgatcatgga ttccatcatc
       61 tgcgtggcgg tgatggtgag cttgttgcgg tcgcgcgcag cgcggatcat cttcttctgc
      121 agcgccggca ccgcggcgtc gcccacctcc accgcgaggt cgccgcgcgc caccatgatg
      181 ccgtcggagg cgtcgaggat ctcgtcgagg ttggccaccg cctcggtgcg ctcgatcttg
      241 gcgatcagca gcgccgaaca gccggcggcg cgcatgagct ggcgcgccat gtacatgtcg
      301 gctgcgctct tggggaagga caccgcgacg aagtccacgc cgatcagcgc ggcggtgcgg
      361 atgtcgtcca tgtccttggc ggtgagcgcg ggcgcggtga gcccgccgcc ctggcggttg
      421 atgcccttgt tgttggacag ctcgccgccc accttcaccg tggtgtggat ctcgtggccg
      481 agcacgcgct gcacggtgag cttgaggcgg ccgtcgtcga gcagcagcac gtcgcccggc
      541 ttcacgtcct tgggcaggtc cttgtagtcg aggccgacgc gctggacgtt gccgagctcg
      601 cagcgggcgt cgaggatgaa gggcgcgtcg cgggtcagcg tcacgcggcc ttcctcgaac
      661 ttgccgacgc ggatcttggg gccctgcagg tcggcgagga tgccgatcgg gcggccggcg
      721 cgcgcggagg cgtcgcggat ggtctctgcg cgggcgacgt ggtcctcggg cttgccatgg
      781 gagaagttca tccgcaccac gtcgaggccg gcgtgcacca tgcgctcgag ggtgaggagg
      841 tcggacgagg cggggccgag ggtggcgacg atcttggtgt ggcgggacat ccgggctgct
      901 ccggtcgttg gcgtgcccgc attctagcag cgcggccgcg acggcgactt cgcgcgcacc
      961 gtacgcgagg cttgcggatc ggcctgcatg gcatatattg gatgaagtct gttcgcggat
     1021 ggcgcccggg taccggagcc agcctcgcca cccactggag gagcgtcatg gccctacccg
     1081 gcaaaccgag ccgtcgcaca gccgcgatgg cggtgtcgag cgtcgtgttg tgcatgctgt
     1141 ccgcaagcgc ggcggccgtg ccgtccagcc tgtacgtgtt cggcgacagc ctctcggaca
     1201 acggcaacct gtatgcctgg acttcgaatc cgtcgaatcc ggtgaccttc ggcttgccga
     1261 tcccggtgtc gcccccctat gagcagggaa ccttcaccaa tggcccggta tacgcggagc
     1321 gcctttggga caggctcggc cttcccgggg agctcgctcc ggcggcgctg ccgggcggca
     1381 cgaaccatgc ggtaggcggg gcgcgctcgc gctaccactc cttcgatgtc ggccccgacg
     1441 gcctgccgcc gctcgtgtgg cccgccaact tcgcgtcctt cagcctgacc ggacagttcc
     1501 agggctaccg cgacgggctc gccggtggcg ctgcggacgc gtcggcgctg tatctggtct
     1561 gggccggtgc gaacgacctg caggacgtgc tccggctcgc ccagctgagc ggccccgccg
     1621 cggccggtgc gcggctggcc gaggcggtcg gcgatgtggc cggcgtgatc ggcgggctgg
     1681 tcggcctg
//
LOCUS       DAOQNN010000608         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_65893_length_1685_cov_11.939877, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000608 DAOQNN010000000
VERSION     DAOQNN010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65893_length_1685_cov_11.939877"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1320)
                     /locus_tag="PKV97_17860"
     CDS             complement(97..1320)
                     /locus_tag="PKV97_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNB07806.1"
                     /translation="MPGALSHIRVLDLSRILAGPWAGQMLADLGADVIKVERPGAGDD
                     TRGWGPPWLKDEQGADTDVAAYYLCANRNKRSITIDITQAEGQALVRRLAAESDVVLE
                     NFKVGGLAQYGLDYDSLKAVNPRLVYCSITGFGQDGPYAPRAGYDFLIQGLGGLMSIT
                     GRPDGEEGGGPMKVGVALTDILTGLYATNAVLAALAWRERSGEGQYIDMALLDVQVAC
                     LANQAGNYLATGQSPKRLGNAHPNIVPYQDFPTADGYMILAIGNDGQFARFCAAAGAP
                     QLATDERFATNRARVVNRATLIPLLKKLTVERGTAEWIAKLEALAVPCGPINTLADVF
                     ADPQVQAREMKVTMPHPVAGQLPLVASPMKLSATPVDYRLPPPMLGEHTDEILAATLG
                     LDAGAIARLRADGVV"
     gene            complement(1338..>1685)
                     /locus_tag="PKV97_17865"
     CDS             complement(1338..>1685)
                     /locus_tag="PKV97_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB07807.1"
                     /translation="FGRPLAANQLIQKKLADMLTEITLGLQAVLRVGRMKDEGIAPVE
                     ITSIIKRNSCGKALDIARMARDMLGGNGISDEYPVARHLVNLEVVNTYEGTHDVHALI
                     LGRAITGLAAFSN"
BASE COUNT          232 a          552 c          613 g          288 t
ORIGIN      
        1 acgcgaacct tgacgttgta atccaccacg cgctttacgg gtacgagaat cttcatgctg
       61 ttcccttgtg ttcgtcttct gttttcttgt tccggctcag accacgccgt ccgcacgcag
      121 gcgggcgatc gccccggcat cgagaccgag cgttgcggcg aggatctcgt cggtgtgctc
      181 gccgagcatc ggcggcggca ggcggtagtc caccggcgtg gccgagagct tcatcgggct
      241 ggcgacgagc ggcaactggc cggcgaccgg atgcggcatc gtcaccttca tctcgcgcgc
      301 ctgcacctgc gggtcggcga agacgtcggc cagggtgttg atcggaccgc agggcacggc
      361 gagcgcctcg agcttcgcga tccactccgc ggtgccgcgc tcgacggtga gcttcttcag
      421 cagcgggatc agcgtggcgc gattgaccac gcgcgcgcgg ttggtggcga agcgctcgtc
      481 ggtcgcgagc tgcggcgcac cggcggccgc gcagaagcgc gcgaactgcc cgtcgttgcc
      541 gatggcgagg atcatgtagc cgtcggcggt cggaaagtcc tggtagggca cgatgttggg
      601 gtgcgcattg cccagccgct tcgggctctg cccggtggcg aggtagttgc ccgcctggtt
      661 ggcgaggcag gccacctgca cgtcgagcag tgccatgtcg atgtactggc cttcgccgct
      721 gcgctcgcgc caggccagcg cggcgagcac ggcgttggtg gcgtacaggc cggtgaggat
      781 gtcggtgagc gccacgccca ccttcatcgg gccaccgccc tcctcgccgt cgggccggcc
      841 ggtgatgctc atcagcccgc ccaggccctg gatcaggaag tcgtagccgg cgcgcggcgc
      901 atacggcccg tcctggccga agccggtgat cgaacagtac accaggcgcg gattgaccgc
      961 cttcaggctg tcatagtcga ggccgtactg cgccaggccg ccgaccttga agttctccag
     1021 caccacgtcg gactcggcgg cgagccggcg caccagcgcc tggccctcgg cctgggtgat
     1081 gtcgatggtg atcgagcgct tgttgcggtt ggcgcacagg taatacgccg ccacgtcggt
     1141 gtcggcgccc tgctcgtcct tgagccaggg cggcccccag ccgcgggtgt cgtcgccggc
     1201 acccggacgc tcgaccttga tcacgtccgc gccgagatcg gcgagcatct gcccggccca
     1261 cgggccggcc aggatgcggg acaggtcgag gacgcggatg tgcgacaggg cgccgggcat
     1321 cgggggtctc cggggactca gttgctgaac gcggcgaggc cggtgatggc gcggccgagg
     1381 atcagcgcat gcacgtcgtg ggtgccttcg taggtgttga ccacctcgag gttcaccagg
     1441 tggcgggcca ccgggtactc gtccgagatg ccgttgccgc cgagcatgtc gcgcgccatg
     1501 cgggcgatgt cgagcgcctt gccgcaggag ttgcgcttga tgatcgaggt gatctcgacc
     1561 ggggcgatgc cctcgtcctt catgcggccc acgcgcagca ccgcctgcag gccgagggtg
     1621 atctcggtca gcatgtcggc gagcttcttc tggatgagct ggttggcggc gagcggacgg
     1681 ccgaa
//
LOCUS       DAOQNN010000609         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_66177_length_1681_cov_12.074416, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000609 DAOQNN010000000
VERSION     DAOQNN010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66177_length_1681_cov_12.074416"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1681
                     /locus_tag="PKV97_17870"
     CDS             <1..>1681
                     /locus_tag="PKV97_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNB07808.1"
                     /translation="RKMREALPNAFLFGLTGTPINRADRNTFYAFGADEDEKGYLSRY
                     GFEESIRDGATLRLHFEPRLVDLHIDKAAIDEAYKDLTGGLSDLDRDNLAKTAAKMAV
                     LVKTPERIRRICEDIVQHYQSKVEPNGFKGQIVTFDRESCLLFKAELDKLLPAEATDI
                     VMSVQPGDRKERPEYARYDRSRDEEERLLDRFRDPADPLKLIIVTAKLLTGFDAPILQ
                     AMYLDKPLRDHTLLQAICRVNRTYSEQKTHGLVVDYLGIFDDVAAALEFDDQSVKQVI
                     SNIQELKDKLPEAMQKCLAFFPGVDRSQQGYEGLIAAQQCLPNNDTRDAFAAEYSVLA
                     RIWEALSPDPLLGQYETDYKWLSQIYQSVQPSSGHGKLIWHSLGAKTIELIHQNVHVD
                     AIRDDLDTLVLDADLLEAVLSNPDPKKAKELEIKLNRRLRKHQGNPKFKDLSERLDAL
                     KERFESGQINSVDFLKQLLQIAKETLQAERETPPEEDEDRGKAALTALFNEVKTPETP
                     IIVERVVTDIDEIVRLVRFPGWQGTQAGEREVKKALRKALFKYKLHADEEL"
BASE COUNT          347 a          574 c          519 g          241 t
ORIGIN      
        1 gccgcaagat gcgcgaggcg ctgcccaatg ccttcctgtt cggcctcacc ggcaccccga
       61 tcaaccgcgc cgaccgcaac accttctacg ccttcggcgc cgacgaggac gagaagggct
      121 acctgagccg ctacggcttc gaggagtcga tccgcgacgg cgccacgctg cggttgcact
      181 tcgagccgcg gctggtggac ctgcacatcg acaaggccgc gatcgacgag gcctacaagg
      241 acctgaccgg cggcctgtcc gacctcgacc gcgacaacct cgccaagacc gccgccaaga
      301 tggcggtgct ggtgaagacg cccgagcgca tccgccgcat ctgcgaggac atcgtccagc
      361 actaccagag caaggtcgag cctaacggct tcaagggcca gatcgtgacc ttcgatcgcg
      421 agtcctgcct gctgttcaag gccgagctcg acaagttgct gccggccgag gcgaccgaca
      481 tcgtcatgtc ggtgcagccg ggcgaccgca aggagcgtcc cgaatacgcc cgctacgacc
      541 gcagccgcga cgaggaagag cgcctgctcg accgctttcg cgacccggcc gacccgctca
      601 agctgatcat cgtcaccgcc aagctgctca ccggcttcga cgcccccatc ctgcaggcga
      661 tgtacctcga caagccgctg cgcgaccaca cgctgctgca ggcgatctgc cgggtgaacc
      721 gcacctattc cgagcagaag acgcacggcc tcgtcgtcga ttacctgggc atcttcgacg
      781 acgtggctgc cgccctggag ttcgacgacc agagcgtgaa gcaggtcatc agcaacatcc
      841 aggagctgaa ggacaagctg cccgaggcaa tgcagaaatg cctcgccttt ttccccggcg
      901 tcgaccgcag ccagcaaggc tacgaaggct tgatcgccgc ccagcagtgc ctgccgaaca
      961 acgacacccg cgacgccttc gccgcggaat acagcgtgct cgcgcgcatc tgggaagcgc
     1021 tgtcgcccga tccgctgctt ggccagtacg agaccgacta caagtggctg tcgcagatct
     1081 accagtcggt gcagccctcg agcggccacg gcaagctgat ctggcattcg ctcggcgcca
     1141 agaccatcga gctgatccac cagaacgtgc atgtcgacgc catccgcgac gacctcgaca
     1201 ccttggtgct cgacgccgac ctgctcgagg ccgtgctgtc gaaccctgac ccgaagaagg
     1261 cgaaggaact cgagatcaag ctcaaccgcc ggctgcgcaa gcaccagggc aatccgaagt
     1321 tcaaggatct atccgagcgg ctggatgcgc tcaaggagcg cttcgagtcc ggccagatca
     1381 acagcgtcga cttcctcaag cagctgctgc agatcgccaa ggaaaccctg caggctgaaa
     1441 gggagacccc tcccgaggaa gacgaggacc gcggcaaggc cgcgctgacc gcgctattca
     1501 acgaggtgaa gacccccgag acgccgatca tcgtcgagcg cgtggtgacg gacatcgacg
     1561 agatcgtgcg cctggtgcgc ttcccgggat ggcaggggac gcaggcgggg gaacgggagg
     1621 tgaagaaggc gctgcggaag gccttgttca agtacaagct gcacgcggac gaggagctgt
     1681 t
//
LOCUS       DAOQNN010000610         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_66876_length_1672_cov_13.629561, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000610 DAOQNN010000000
VERSION     DAOQNN010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66876_length_1672_cov_13.629561"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PKV97_17875"
     CDS             complement(<1..649)
                     /locus_tag="PKV97_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB07809.1"
                     /translation="MTHLKITGMTCDSCAAHVKEALEKVPGVQSALVSYPKGTAQLAI
                     VPGTSPDALTAAVAGLGYKATLADAPLADNRVGLLDKVRGWMAAAEKHSGNEPPVQVA
                     VIGSGGAAMAAALKAVEQGAQVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRES
                     PFDGGIAATVPTIDRSKLLAQQQARVDELRHAKYEGILGGNPAITVVHGEARFKDD"
     gene            complement(721..996)
                     /gene="merP"
                     /locus_tag="PKV97_17880"
     CDS             complement(721..996)
                     /gene="merP"
                     /locus_tag="PKV97_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HNB07810.1"
                     /translation="MKKLFASLALAAVVAPVWAATQTVTLSVPGMTCSACPITVKKAI
                     SKVEGVSKVDVTFETRQAVVTFDDAKTSVQKLTKATADAGYPSSVKQ"
     gene            complement(1009..1359)
                     /gene="merT"
                     /locus_tag="PKV97_17885"
     CDS             complement(1009..1359)
                     /gene="merT"
                     /locus_tag="PKV97_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric ion transporter MerT"
                     /protein_id="HNB07811.1"
                     /translation="MSEPKTGRGALFTGGLAAILASACCLGPLVLIALGFSGAWIGNL
                     AVLEPYRPIFIGVALVALFFAWRRIYRQAAACKPGEVCAIPQVRATYKLIFWIVAALV
                     LVALGFPYVMPFFY"
     gene            1431..>1672
                     /locus_tag="PKV97_17890"
     CDS             1431..>1672
                     /locus_tag="PKV97_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNB07812.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLLEPDKPYGSIR
                     RYGEADVTRVRFVKSAQRLGFSLDEIAELLRLEDGT"
BASE COUNT          312 a          505 c          532 g          323 t
ORIGIN      
        1 ggtcgtcctt gaagcgcgcc tcaccgtgca caacggtgat ggccggatta ccgcccagga
       61 tgccttcgta cttggcgtgc cgcagttcgt cgacgcgggc ctgctgctgg gccagcagct
      121 tactgcggtc aatcgtaggc acagttgccg caataccgcc atcgaacggg ctttcccggc
      181 gcagatgggc gatgtgggcg gcgcggatca tgatcttgga cggcacacag ccgacattga
      241 cgcaggtgcc gccgatggtg ccgcgctcga tcagcgtgac ctgcgcgcct tgctcgacgg
      301 ccttcagcgc cgccgccatc gcggctccac cgctgccaat gaccgctacc tgcaccgggg
      361 gctcgttgcc actgtgcttt tcggcggcgg ccatccatcc ccgcaccttg tcgagcagtc
      421 cgacgcggtt gtccgccagt ggcgcatcgg ctagcgttgc cttgtagccc agtccggcca
      481 cggcggcagt cagcgcgtcc ggcgatgtgc ccggcacgat ggcgagttgc gctgtgccct
      541 tcggatagga caccagcgcc gactgcacgc ctggcacttt ttccagcgct tccttgacgt
      601 gcgccgcgca cgagtcgcaa gtcatgccgg tgatttttag atgggtcatg caacagatcc
      661 tttatcgttt gtggcgccag acaatgacgt ccgttgtgct gcggtgccgt tttcagtgac
      721 tcactgcttg acgctggacg gatagcccgc gtctgcggtt gccttggtca gcttctgcac
      781 gctggtcttg gcatcgtcga aggtgacgac cgcttggcgt gtctcgaaag tcacgtcaac
      841 tttgctgacg ccttcgacct tggaaatcgc cttcttgaca gtgatcgggc aggcggagca
      901 ggtcatgccc ggtacggaca gcgtgacggt ctgggtggcg gcccagacgg gggcaacaac
      961 ggcggcgagg gcgagggagg caaacagttt cttcatggtg aactccgatc agtagaaaaa
     1021 tggcatgacg tagggaaatc cgagcgcgac cagaaccagc gcggccacga tccagaaaat
     1081 gagcttgtaa gtagctcgca cttggggaat cgcgcagacc tcacccggtt tgcaggccgc
     1141 tgcctgccgg tagatgcgcc gccaggcgaa gaacaacgcc accagcgcca cgccgataaa
     1201 gatggggcga tagggttcca acaccgccaa gttgccgatc caagcgccgc tgaaccccaa
     1261 ggcgatcaga accaacggcc cgaggcagca agccgaggcg aggatggcgg caagccctcc
     1321 agtgaagagc gcgccgcgcc cggtttttgg ttcagacatg cgcttgtcct ttcgaattga
     1381 aattggatag cgtaacctta cttccgtact catgtacgga gtcaagcgat atggaaaaca
     1441 atttggagaa cctgaccatt ggcgttttcg ccaaggcggc cggggtcaat gtggagacca
     1501 tccgtttcta tcagcgcaag ggcttgttgc tggagcctga caagccctat ggcagcatcc
     1561 gccgctatgg cgaggcggat gtaacgcggg tgcgcttcgt gaaatcagcc cagcggctgg
     1621 gcttcagcct ggatgagatc gccgagctgc tgcggctgga ggatggcacc ca
//
LOCUS       DAOQNN010000611         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_67033_length_1670_cov_6.458824, whole genome shotgun sequence.
ACCESSION   DAOQNN010000611 DAOQNN010000000
VERSION     DAOQNN010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67033_length_1670_cov_6.458824"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PKV97_17895"
     CDS             <1..600
                     /locus_tag="PKV97_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNB07813.1"
                     /translation="VSALIGEWLVPRLHGLYARHPELRVDLVASNDNLNVARREADIA
                     IRLARPASGDFLIRKLAGVGFALYGAARGDLAVSAGDWVAYNEDLDHTPEMRRLHAML
                     GEGRIRLRSNSLRGLVRAVAGGIGHGLLPCFVADADPGLVRLSGAEPILYRELWMLIH
                     PDARPLARVAATADWLVERFDEAAAGFAGRVPSPRSAPP"
     gene            complement(631..1212)
                     /locus_tag="PKV97_17900"
     CDS             complement(631..1212)
                     /locus_tag="PKV97_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNB07814.1"
                     /translation="MTIRFRSVLAASALATLSGAALAHPGHAPASFASGFAHPLGGLD
                     HLLAMLAVGLYAARQAGAARLLLPTGFVLAMLAGAGLGALGLALPAVEAGISASVLVL
                     GLLIAFAARLPLAASLPLVAAFALFHGHAHHAEMGDATLLGYSLGFALATAALHAAGL
                     TLARAFPDSRGGRLALRLGGGGIAGVGVALLGG"
     gene            complement(1251..>1670)
                     /gene="ureG"
                     /locus_tag="PKV97_17905"
     CDS             complement(1251..>1670)
                     /gene="ureG"
                     /locus_tag="PKV97_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HNB07815.1"
                     /translation="ETGGCPHTAIREDASINLEAVERLNARFPGLDIVFVESGGDNLA
                     ATFSPELSDLTIYVIDVSAGDKIPRKGGPGITRSDLLVINKIDLAPLVGASLEVMDRD
                     ARRMRGDRPFLFSNQKTGQGLREIIDFIERQGMLAAA"
BASE COUNT          224 a          577 c          653 g          216 t
ORIGIN      
        1 gtgagcgccc tgatcggcga gtggctggtg ccgcggctgc acggcctgta cgcccgtcat
       61 ccggagctgc gcgtcgacct cgtcgccagc aacgacaacc tcaacgtcgc gcgccgcgag
      121 gccgacatcg ccatccgcct ggcgcggccg gcgagcggcg acttcctcat ccgcaagctc
      181 gccggggtcg gcttcgcgct ctacggcgcg gcgcgcggcg acctggcggt gagcgccggc
      241 gactgggtgg cctacaacga ggacctcgac cacaccccgg agatgcgccg cctgcacgcc
      301 atgttgggcg agggccgcat ccgcctgcgc tcgaacagcc tgcgcggcct cgtgcgcgcg
      361 gtggccggcg gcatcggcca cggcctgcta ccctgcttcg tcgccgatgc ggaccccggc
      421 ctggtccgcc tgtccggcgc ggagccgatc ctgtaccgtg agctgtggat gctgatccac
      481 cccgacgccc gtccgctcgc gcgcgtcgcc gcgaccgcgg actggctggt ggagcgcttc
      541 gacgaggcgg cggcgggttt cgccggcagg gtgccgtctc cccggagcgc gccgccctag
      601 caccggcgcg gcggtcttgc cgcggaggct tcagccgccg agcagggcga cgcccacgcc
      661 ggcgattccc ccgccgccca ggcgcagcgc aaggcgtccg ccgcggctgt cggggaaggc
      721 gcgcgcgagc gtcaggccgg ccgcgtgcag ggccgcggtc gcgagtgcga agccgaggct
      781 gtagccgagc agggtggcgt cgcccatctc ggcgtggtgg gcatggccgt ggaagagcgc
      841 gaaggcggcg accaggggca ggctcgccgc cagcggcagg cgggcggcga atgcgatcag
      901 caggcccagc accagcaccg aggcggaaat gccggcctcg accgcgggca gggcgaggcc
      961 gagcgcgccg aggccggcgc cggcgagcat cgccagcacg aagccggtgg gcagcagcag
     1021 gcgtgcagcg cccgcctggc gtgcggcgta gaggcccacc gccagcatgg cgagcagatg
     1081 gtcgagcccg ccgagcggat gggcgaagcc gctggcgaag ctggcgggtg cgtgcccggg
     1141 gtgggcgagc gcggcccccg agagggttgc gagcgcactg gcggcgagca cggagcggaa
     1201 gcggatcgtc atggtggatt ctccaggagt gcgggaaggt cttgcggtgt tcaggcggcg
     1261 gccagcatgc cctggcgctc gatgaagtcg atgatctcac gcaggccctg tccggtcttc
     1321 tggttcgaga acaggaaggg gcgatctccc cgcatcctgc gcgcgtcgcg gtccatcacc
     1381 tcgagcgagg cgcctaccag cggcgcgagg tcgatcttgt tgatcaccag caggtcgctg
     1441 cgggtgatgc ccggcccgcc cttgcgcggg atcttgtcgc cggcggagac gtcgatcacg
     1501 tagatcgtca ggtcggagag ctcgggcgag aaggtggcgg cgaggttgtc gccgccggat
     1561 tcgacgaaga cgatgtccag cccggggaag cgcgcgttca ggcgctcgac cgcctcgagg
     1621 ttgatcgagg cgtcctcgcg gatcgcggtg tgcgggcagc cgccggtctc
//
LOCUS       DAOQNN010000612         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_67507_length_1664_cov_8.136109, whole genome shotgun sequence.
ACCESSION   DAOQNN010000612 DAOQNN010000000
VERSION     DAOQNN010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67507_length_1664_cov_8.136109"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="PKV97_17910"
     CDS             <1..96
                     /locus_tag="PKV97_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNB07816.1"
                     /translation="NTRSHVWYRYFNEIPVLVLFVVVFLVVLKPF"
     gene            99..1604
                     /locus_tag="PKV97_17915"
     CDS             99..1604
                     /locus_tag="PKV97_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB07817.1"
                     /translation="MVAKRIGGGGDDVQMARGVRGDEAKALAQGGVGRAEPARPLELA
                     DYNWPAHSVLVVDDEEGMRNFLERTLARRCGMVQSAADAEHAAVLMARLHFDLLVLDI
                     ALPGKSGIEWLHELRENGYAGDVILITAFADMETAIDALRGGASDFILKPFRVDQILN
                     SIKRCFERAGLARENFVLRRELAGLGAEPTGLIGHSPAMEQLRALVRRVGQMPSTVLL
                     LGESGTGKEVVARALHQTSPRAQRPFVPLNCAAIASELIESELFGHVKGAFTGATENR
                     NGLFYYAHGGTLFLDELSELPLAMQTRLLRVLEERKLRPVGSERELPVDVRIIAASNR
                     DLAAEVRAGRFREDLYYRLAVVDIGLPPLRDRTEDIPELMRHFMQQFSVQLGVPPLPL
                     SHEVVSRLAGYTWPGNVRELRNYIERSLILGHFPAQPSAAPTAAPTPAGELEASLAEV
                     ERRHIERVTAACEGNKTEAARRLGVSRKTLERKFAEWALEDAAAARAERRA"
BASE COUNT          232 a          584 c          604 g          244 t
ORIGIN      
        1 aacacgcgca gccacgtgtg gtaccgctat ttcaacgaga tcccggtgct ggtgctgttc
       61 gtggtggtgt tcctcgtggt cttgaagccc ttctgaacat ggtcgcgaaa cggatcgggg
      121 gcggcgggga cgacgtgcag atggcgcgcg gcgtgcgggg cgacgaggcg aaggcgctcg
      181 cgcaaggcgg agtcgggcgc gccgagcccg cgcgcccgtt ggagctggcc gactacaact
      241 ggccggcgca ctcggtgctg gtggtcgacg acgaggaggg catgcgcaat ttcctcgagc
      301 gcacgctcgc gcgccgctgc ggcatggtgc agtcggccgc cgatgccgag cacgccgcgg
      361 tgctgatggc gcgtctgcat ttcgacctgc tggtcctcga catcgccctg cctggcaagt
      421 cggggatcga gtggctgcac gagctgcgcg agaatggtta tgccggcgac gtgatcctga
      481 tcaccgcctt cgccgacatg gagaccgcga tcgacgcgct gcgcggcggc gcctcggact
      541 tcatcctcaa gcccttccgc gtcgaccaga tcctcaactc gatcaagcgc tgcttcgagc
      601 gtgccggcct ggcgcgcgag aacttcgtgc tgcgccgcga gctcgccggc ctgggcgccg
      661 agcccaccgg gctgatcggc cactcgcccg cgatggagca gctgcgcgcg ctggtgcgcc
      721 gcgtcgggca gatgccgagc acggtgctgc tgctgggcga atccggtacc ggcaaggagg
      781 tggtggcgcg cgcgttgcac cagaccagcc cgcgcgcgca gcgccccttc gtgccgctca
      841 actgcgcggc gatcgcctcc gagctgatcg agagcgagct cttcggccac gtcaagggcg
      901 ccttcaccgg cgccaccgag aaccgcaacg gcctgttcta ctacgcccac ggcggcacgc
      961 tcttcctcga tgagctcagc gagctgccgc tggcgatgca gacccggctg ctgcgggtac
     1021 tcgaggagcg caagctgcgc ccggtcggtt ccgagcgcga gctgccggtg gatgtacgca
     1081 tcatcgccgc ctccaaccgc gacctcgccg ccgaggtgcg cgccggacgc ttccgcgagg
     1141 acctctacta ccgcctcgcg gtggtggaca tcggcctgcc gccgctgcgc gaccgtaccg
     1201 aggacatccc cgagctgatg cgccacttca tgcagcagtt ttccgtccag ctcggcgtgc
     1261 cgccgctgcc gctctcgcac gaggtggtga gccggctcgc cggctacacc tggcctggca
     1321 acgtgcgcga actgcgcaac tacatcgagc gctcgctgat tctcggccac ttcccggcgc
     1381 agccgagcgc cgcaccgacc gccgcgccga cgccggcggg cgagctggag gcgagcctgg
     1441 ccgaggtcga gcgccgccac atcgagcgtg tgaccgccgc ctgcgagggc aacaagaccg
     1501 aggccgcgcg ccggctgggc gtgtcgcgca agaccctgga gcgcaagttc gccgagtggg
     1561 cgctcgagga cgccgcggcc gcgcgtgcgg agcggcgggc ctgagcgtgg cgggggtcgg
     1621 ggcgagcagg cggcggacgg ggccggagcc atccggcgtg cgtc
//
LOCUS       DAOQNN010000613         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_68122_length_1656_cov_8.670206, whole genome shotgun sequence.
ACCESSION   DAOQNN010000613 DAOQNN010000000
VERSION     DAOQNN010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68122_length_1656_cov_8.670206"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PKV97_17920"
     CDS             complement(<1..505)
                     /locus_tag="PKV97_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNB07818.1"
                     /translation="MALLQITEPGMSTEPHQHRLAVGIDLGTTNSLVATVRNGIAICL
                     PDEAGRTMLPSAVRYHADGGIEVGLGALKAQAVDPRNTIVSVKRFMGRGLKDVTHIEA
                     MPYDFEDSPGMVRLRTVQGVKSPVEVSAEILRRLRERAEASLGGALVGAVITVPAYFD
                     DAQRQATK"
     gene            complement(533..1066)
                     /gene="hscB"
                     /locus_tag="PKV97_17925"
     CDS             complement(533..1066)
                     /gene="hscB"
                     /locus_tag="PKV97_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNB07819.1"
                     /translation="MSIDLTQDYFALFGLPRRYAIDDGALEAAWHELQGRVHPDRHAH
                     LSDFEKRRSMQWATRVNEGFRVLRKPLARAQYLLELAGVDAALETNTAMSPEFLMEQM
                     EWREAVEEAREAAEVSELEDLHRRLLGHAREVRGHLAAQLDEHQDYEAAADTVRRLMF
                     IEKLQQEIDEALLALEN"
     gene            complement(1077..1400)
                     /gene="iscA"
                     /locus_tag="PKV97_17930"
     CDS             complement(1077..1400)
                     /gene="iscA"
                     /locus_tag="PKV97_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HNB07820.1"
                     /translation="MAVTLSESAARHVSNFIAKRGKGFGIRLGVKTSGCSGMAYKLEF
                     VDQTEDEDEVFESHGVKVVIDPKSLAYLDGTELDFVKEGLNEGFKFNNPNVKDQCGCG
                     ESFNV"
     gene            complement(1427..>1656)
                     /locus_tag="PKV97_17935"
     CDS             complement(1427..>1656)
                     /locus_tag="PKV97_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNB07821.1"
                     /translation="IEDAKFKTYGCGSAIASSSLVTEWVKGKTLDQALDIKNTQIAEE
                     LALPPVKIHCSILAEDAIKAAVADYKKKHEA"
BASE COUNT          242 a          545 c          553 g          316 t
ORIGIN      
        1 ccttggtggc ctggcgctgg gcgtcgtcga agtaggccgg cacggtgatc accgcgccga
       61 ccagcgcgcc gccgaggctg gcctcggcgc gctcgcgcag gcggcgcagg atctcggcgg
      121 agacctccac cgggctcttg acgccctgca ccgtgcgcag ccgcaccatc ccggggctgt
      181 cctcgaagtc gtagggcatc gcctcgatgt gggtgacgtc cttgaggccg cggcccatga
      241 agcgcttgac cgagacgatg gtgttgcgcg gatcgaccgc ctgcgccttg agcgcaccca
      301 gaccgacctc gatgccgcca tcggcgtgat agcgcacggc cgagggcagc atggtgcggc
      361 cggcctcgtc gggcaggcag atcgcgatgc cgttgcgcac ggtggcgacg agcgaattgg
      421 tggtcccgag gtcgatgccg actgcgagcc ggtgctggtg cggctcggtg gacatgccgg
      481 gttcagtgat ttgcaacaga gccatcggtg ctgttccgtg ccttttcgag ttctagtttt
      541 cgagcgcgag cagcgcttcg tcgatttcct gctggagctt ctcgatgaac atcagccgac
      601 gcacggtgtc ggccgcggcc tcgtagtcct ggtgctcgtc gagctgcgcg gcgagatgcc
      661 cgcgcacctc gcgcgcatgc ccgagcaggc gcctgtgcag atcttcgagc tcggagacct
      721 cggccgcctc gcgcgcctcc tccaccgcct cgcgccactc catctgctcc atcaggaact
      781 cgggggacat cgcggtgttg gtctcgagcg cggcgtcgac gccggcgagc tcgagcaggt
      841 actgggcgcg cgcgagcggc ttgcgcagca cgcgaaagcc ttcattgacc cgcgtcgccc
      901 actgcatcga gcgccgcttc tcgaagtcgg acaggtgggc gtggcggtcg ggatgcacgc
      961 gcccctgcaa ctcgtgccaa gcggcttcga gcgcgccgtc gtcaatcgcg tagcggcgcg
     1021 gcagaccgaa gagggcgaag tagtcctgcg tcaggtcgat gctcatcggg tccggctcag
     1081 acgttgaagc tctcgccgca cccgcactgg tccttgacgt tcgggttgtt gaacttgaag
     1141 ccctcgttga ggccttcctt gacgaagtcg agctcggtgc cgtcgagata ggccaggctc
     1201 ttggggtcga tgacgacctt gacgccgtgg ctctcgaaga cctcgtcctc gtcttccgtc
     1261 tggtcgacga actcgagctt gtaggccatg cccgaacagc ccgaggtctt cacgcccaga
     1321 cggatgccga agcccttgcc gcgcttggcg atgaagttcg agacgtggcg cgcagccgat
     1381 tcagatagcg tgacagccat gtttcctcct cttcgacgaa ggccgctcag gcctcatgct
     1441 tctttttata gtccgccacg gcggccttga tcgcatcttc ggcgaggatc gagcagtgga
     1501 tcttcaccgg cggcagcgcg agctcctcgg cgatctgcgt gttcttgata tcgagcgcct
     1561 ggtcgagggt cttgcccttg acccattcgg tgaccagcga gctcgacgcg atcgccgagc
     1621 cgcagccgta ggtcttgaac ttcgcgtcct cgatca
//
LOCUS       DAOQNN010000614         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_68360_length_1653_cov_8.440551, whole genome shotgun sequence.
ACCESSION   DAOQNN010000614 DAOQNN010000000
VERSION     DAOQNN010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68360_length_1653_cov_8.440551"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PKV97_17940"
     CDS             complement(<1..423)
                     /locus_tag="PKV97_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HNB07822.1"
                     /translation="MDMTPQTAWLLAISAVVIVGVIAFFVGRSSAGTKGRIDELEAEL
                     ARKAEEAERYRKEVDAHFDKTATLFVSMAGSYKELFEHLSAGYDKLSGASARERFQER
                     VDALLPRRVVEPAAAGALLAGGAAGAVAGADAAEPTGSS"
     gene            complement(448..1482)
                     /locus_tag="PKV97_17945"
     CDS             complement(448..1482)
                     /locus_tag="PKV97_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNB07823.1"
                     /translation="MADALRVLALVACALLLAACSRAQVFHHEAYVFGTRVDVAVYGE
                     NQAEADRAAAAVLREFDRLHRAYHAWEPSELTALNAAIARGEAATVSDELAAMLKDAQ
                     AIAATGDGLFDPALGGLVALWGFHTDTFVPQRPDPAKLQALLAAHPSMADLVLDGNRV
                     ESRNPAVQLDLGGYAKGYALDRAAAILRAQGVADALINIGGNVMALGAKGDQPWRIGI
                     QHPRAPAPLATLPLYDGEAIGTSGDYQRYFELHGERYAHLLDPRTGQPAHGTQSLTVL
                     ITPRANAGTLSDAPSKPAYLAGDGWREQIRRFGIEHALRVAADGRVEVTRALRARLQF
                     PSPVEATVVD"
     gene            complement(1524..>1653)
                     /locus_tag="PKV97_17950"
     CDS             complement(1524..>1653)
                     /locus_tag="PKV97_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNB07824.1"
                     /translation="DVIGGHLTEINVTSPTCMVEIATQQGFDVAGLVIDKLERACG"
BASE COUNT          234 a          565 c          623 g          231 t
ORIGIN      
        1 gctcgatccc gtcggttccg cggcgtccgc acccgcaaca gcgcccgccg ctccccccgc
       61 gagcagcgcg ccggccgcgg cgggctcgac cacgcggcgg ggcagcagcg catcgacgcg
      121 ctcctggaag cgctcgcgcg ccgaggcgcc ggagagcttg tcgtagccgg cggacaggtg
      181 ctcgaagagc tccttgtagg agccggccat cgacacgaac agggtcgcgg tcttgtcgaa
      241 gtgggcgtcg acctccttgc ggtagcgctc ggcctcctcg gccttgcgcg ccagctcggc
      301 ctcgagctcg tcgatgcgtc ccttggtacc ggcgctcgaa cgcccgacga agaacgcgat
      361 cacgccgacg atgacgacgg cggaaatggc gagcagccag gcggtttgcg gggtcatgtc
      421 catgttgtcc tccctccttt gtggatctca atcgaccacc gtcgcctcca ccggcgacgg
      481 gaactgcagg cgcgcgcgca acgcgcgggt gacctcgacg cggccgtccg cagccacgcg
      541 cagtgcatgt tcgatgccga agcggcggat ctgttcgcgc cagccatctc cggcgaggta
      601 ggcgggcttg ctcggtgcat ccgacagcgt gccggcattg gcgcgcggcg tgatcaggac
      661 ggtgagcgac tgcgtgccat gcgcgggctg gccggtgcgc gggtcgagca ggtgggcgta
      721 gcgctcgccg tggagctcga agtagcgctg gtagtcgccc gaggtgccga tcgcctcgcc
      781 gtcgtacaaa ggcagggtgg cgagcggcgc gggcgcgcgc ggatgctgga tgccgatccg
      841 ccagggctgg tcgcccttgg cgccgagcgc catgacgttg ccgccgatgt tgatgagcgc
      901 gtcggccacg ccctgcgcgc gcaggatcgc cgccgcgcgg tcgagcgcgt agcccttggc
      961 gtagccgccg agatcgagct gcaccgccgg gttgcggctc tcgacccgat tgccgtcgag
     1021 gacgaggtcg gccatcgacg ggtgcgcggc aagcagggcc tgcagcttgg cagggtcggg
     1081 gcgctgcggc acgaaggtgt cggtgtggaa gccccacagc gcgaccaggc cgccgagcgc
     1141 agggtcgaac aacccgtcgc cggtggcggc gatcgcctgc gcgtccttca gcatcgcggc
     1201 gagctcgtcg gacacggtgg cggcctcgcc gcgcgcgatc gcggcgttga gcgcggtgag
     1261 ctcggagggc tcccaggcgt ggtaggcgcg atgcaggcgg tcgaactcgc gcagcaccgc
     1321 ggcggcggcg cggtcggcct cggcctggtt ctcgccatac accgcgacat cgacccgggt
     1381 accgaagacg taggcctcgt gatggaagac ctgcgcgcgc gagcaggccg cgagcagcag
     1441 cgcacaggct accaacgcga ggacgcgcag cgcgtccgcc acagcacgcg gatcggagaa
     1501 ggcggagagg ctcgggcgca ggctcagccg cacgcccgct ccagcttgtc gatcaccagg
     1561 ccggccacgt cgaagccctg ctgggtggcg atctccacca tgcaggtggg gctggtgacg
     1621 ttgatctcgg tgaggtggcc gccgatcacg tcg
//
LOCUS       DAOQNN010000615         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_68493_length_1651_cov_7.964912, whole genome shotgun sequence.
ACCESSION   DAOQNN010000615 DAOQNN010000000
VERSION     DAOQNN010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68493_length_1651_cov_7.964912"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PKV97_17955"
     CDS             complement(<1..1221)
                     /locus_tag="PKV97_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB07825.1"
                     /translation="MSPVFMLSLRSIANRRLSAALTVVAVALAVALLLGVERLRNDAR
                     AGFAQTISATDLVVGARSGPVQLLLYSVFHIGDATANLSWASIEHVAALPQVKWLVPL
                     ALGDSHRGHRVVGTTTDFFAQYRHGEGRSLVFAAGGPFAGATARVEDLFQTVIGAEVA
                     ARHGYRLGERIVLSHGGGAGEGGARFAEHADKPFTVVGILAPSATPLDRAVLVSLEGL
                     EAIHVDWHGGAPIPGLKITPEQVRKFDLRPKSVTAALVGLHSRSAVFRVQRQINAYAG
                     EPLTAILPGATLQQLWDLVGIAERALLAVSALVVVVGLTGMVAVVLASLGERRRELAI
                     LRALGASPREVFALLALESLLLATAGIALGLGLLYGAGAALAPWLAAQHGLQPSLGWP
                     AAGEWRLLGAVLAAS"
     gene            complement(1218..>1651)
                     /locus_tag="PKV97_17960"
     CDS             complement(1218..>1651)
                     /locus_tag="PKV97_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB07826.1"
                     /translation="LGGQELGVERTEQLADRLGAGDQQLAGGLRIDLAGETFEDQARL
                     LTHELLERPATQLSVGQQQRVAAARALIGRPGLVVADEPTSALDADRQQTFIDLLLDE
                     CAAVGAALLFVSHDQRLAAHFDRVLDLGALVRAGLAAEDAR"
BASE COUNT          244 a          593 c          625 g          189 t
ORIGIN      
        1 gctggcggcg agcaccgcgc cgagcaggcg ccattcgccc gccgcgggcc agcccaggct
       61 cggctgcaga ccatgctgcg cggcgagcca gggggcgagc gcggcgcccg caccatacag
      121 cagcccgagg ccgagcgcga tgcccgcagt ggcgagcagc aggctctcaa gcgcgagcag
      181 cgcgaagacc tcgcgcgggc tggcacccag cgcacgcagg atggcgagct cgcggcggcg
      241 ctcgcccagg cttgccagca ccaccgccac catgcccgtc aggccgacca cgaccaccag
      301 ggccgagacc gcgagcagcg cgcgctcggc gatgccgacg aggtcccaca gctgctgcag
      361 ggtcgcaccg ggcaggatgg cggtgagcgg ttcgccggcg taggcgttga tctggcgctg
      421 cacgcggaac accgcgctgc ggctgtgcag cccgacgagc gcggccgtga cggatttggg
      481 gcgcaggtcg aacttgcgca cctgctcggg cgtgatcttc aggcccggga tgggcgcgcc
      541 gccatgccaa tcgacatgaa tcgcctccag gccctccagg ctcaccagca cggcgcggtc
      601 gagcggcgtg gcgctgggcg cgaggatgcc gaccacggtg aagggcttgt cggcgtgctc
      661 ggcgaagcgc gctccgccct cccccgcgcc gccgccgtgg ctgagcacga tgcgctcgcc
      721 gaggcggtaa ccatggcggg cggcgacctc ggcgccgatc acggtctgga agaggtcctc
      781 gacgcgcgcg gttgcgccgg cgaacgggcc gccggccgcg aagaccagac tgcgcccctc
      841 gccatggcgg tattgcgcga agaagtccgt ggtggtgccg accacgcggt gaccgcggtg
      901 cgagtcgccg agcgcgagcg gtaccagcca cttcacctgg ggcagcgcgg cgacgtgctc
      961 gatgctggcc cacgacaggt tggcggtggc gtcgccgatg tggaagaccg agtacagcag
     1021 cagctgcacc gggccgctgc gcgcgcccac caccaggtcg gtggcggaaa tcgtctgcgc
     1081 gaagcccgcg cgcgcatcgt tgcgcaggcg ctcgaccccg agcagcagcg ccactgccag
     1141 cgccaccgcg accacggtga gcgcggccga cagcctgcgg ttggcgatgc tgcgcagcga
     1201 cagcatgaag acggggctca tcgcgcgtcc tccgcagcga ggccggcgcg gacgagcgca
     1261 ccgaggtcga gcacgcggtc gaagtgcgct gccaggcgct ggtcgtggct gacgaagagc
     1321 agcgcggcgc cgaccgcggc gcattcgtcg agcagcaggt cgatgaaggt ctgctgacga
     1381 tcggcgtcga gcgcggaggt gggctcgtcc gccaccacca ggccgggacg gccgatcagc
     1441 gcgcgcgccg cggctacgcg ctgctgctgg ccgaccgaga gctgcgtggc cgggcgctcg
     1501 agcagctcgt gcgtgaggag gcgagcctga tcctcgaacg tctcgccggc cagatcgatc
     1561 cggaggccgc ccgcgagctg ctggtctccg gcgccgagcc ggtccgcgag ctgctcggtg
     1621 cgctccacgc caagctcctg gccgccgagc t
//
LOCUS       DAOQNN010000616         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_68563_length_1650_cov_10.895298, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000616 DAOQNN010000000
VERSION     DAOQNN010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68563_length_1650_cov_10.895298"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..>1650)
                     /locus_tag="PKV97_17965"
     CDS             complement(143..>1650)
                     /locus_tag="PKV97_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07827.1"
                     /translation="KGDDKIRPDGTIEEADSFVLPFQPNRAQRRFIRRLWHRNLILKA
                     RQLGFTTLIAVLWLDHALFNANQRCGIIAQDREAAEAVFRDKVRFAYEHLPSEIRERF
                     PLDRDAASELLFAHNNSSVRVATSMRSGTIHRLHVSEFGKICAKYPDKAQEVVTGSIP
                     AVPMNGVLVIESTAEGRDGDFFKIVQIAEAHEASRKVLTARDYRLHFYAWWMEPKYRI
                     DAYTVDLTREDHEYFEKVEIEVKKALGLDLRLDAEQKAWYVATKRADFSGAEERMWQE
                     YPSYPAEAFQVSTEGNWYAKDMIELRKRGGITRVPSLDLPVNTFWDIGNSDGTAIWFH
                     QDLRGEDRFIDYYEGHNEDLRHYVAELRAKGFVFDTHYLPHDADHKRLSDYNRSTREM
                     LQDLMPGERFAIVPLITELVTGIQQTRKHLKGAYFDEAGCDKGIKRIEGYRKRFNRAE
                     GRYTSDPDKSNGCSEGADALRQWAQAKELGMVGSASKQQSYDEPPPPDWRT"
BASE COUNT          240 a          499 c          558 g          353 t
ORIGIN      
        1 tcgcgcgcca gggcggctgg ttgatggcct cgcggacgaa ctgcgcgaac tcgtcgatcg
       61 tgatcggctc gtcggcgtct tgcagtgcgg tgtcgtcaat ggtgcgggtc tgttctgcga
      121 gcatcatgcg ctccggtggt ggtcaggtgc gccagtcggg cggcgggggt tcgtcgtagc
      181 tttgttgctt ggatgcgctg cccaccatgc ccagctcctt tgcttgcgcc cactggcgca
      241 gggcgtcggc gccctcgctg catccgttgg acttgtccgg gtcgctcgtg tagcggcctt
      301 cagcgcggtt gaagcgtttg cggtagccct cgatgcgctt gatgcccttg tcgcacccgg
      361 cctcatcgaa gtacgcgccc ttgaggtgtt tgcgcgtctg ctggatgccg gtcaccaact
      421 cggtgatgag cggcacgatg gcgaatcgct cgcccggcat gaggtcttgc agcatctcgc
      481 gcgtgctgcg gttgtagtcg gacaggcgct tgtggtcggc gtcgtgcggc aggtagtggg
      541 tgtcgaacac gaaacccttg gcgcggagct cggcgacgta gtggcgcaga tcctcgttgt
      601 ggccttcgta gtagtcgatg aaccgatcct cgccgcgcaa gtcctgatgg aaccagatcg
      661 ccgttccgtc ggagttgccg atgtcccaaa aggtgttgac gggcaagtcc aggctcggca
      721 cgcgggtgat tccaccgcgc ttgcgcagct cgatcatgtc cttcgcgtac cagttgccct
      781 cggtgctgac ttggaatgct tcggcagggt aggaggggta ttcctgccac atccgttctt
      841 cggcgccgct gaagtccgcg cgcttggttg cgacgtacca cgccttctgc tcggcgtcca
      901 gacgaaggtc caggccgagc gccttcttga cctcgatctc gaccttctcg aaatactcgt
      961 ggtcctcgcg tgtcagatcg accgtatagg cgtcgatgcg gtacttcggc tccatccacc
     1021 aggcgtagaa gtgcagccgg tagtcgcgcg ccgtcagcac cttgcggctg gcctcgtgcg
     1081 cctcggcgat ctgcacaatc ttgaagaagt caccgtcgcg gccctcggcg gtgctctcga
     1141 tcaccagcac gccattcatg ggcacggccg ggatggagcc ggtgacgact tcctgggcct
     1201 tgtccgggta cttcgcgcaa atcttgccga actcggaaac gtgcagccgg tggatggtgc
     1261 ccgagcgcat ggaggttgcc acgcgcacgc tgctgttgtt gtgcgcgaaa agcagttccg
     1321 acgcagcatc gcggtcgagc gggaagcgct cgcggatctc gctcggcagg tgctcgtagg
     1381 cgaagcgcac cttgtctctg aaaacggctt cggcggcctc gcgatcctgg gcgatgatgc
     1441 cgcagcgctg gttggcgttg aacagggcgt gatccagcca caggaccgcg atcagcgtcg
     1501 tgaagccgag ctgtcgcgcc ttgaggatca ggtttcgatg ccacagacgg cggatgaatc
     1561 ggcgctgggc gcgatttggc tggaacggca ggacgaacga gtccgcttct tcgatcgtgc
     1621 catccgggcg gatcttgtcg tcgcccttga
//
LOCUS       DAOQNN010000617         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_68644_length_1649_cov_7.769134, whole genome shotgun sequence.
ACCESSION   DAOQNN010000617 DAOQNN010000000
VERSION     DAOQNN010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68644_length_1649_cov_7.769134"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PKV97_17970"
     CDS             complement(<1..433)
                     /locus_tag="PKV97_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNB07828.1"
                     /translation="MAIVRVALPIPLPQAFDYTAADAGVGDVGRCVKVPFGRGERTGL
                     IVGIGDEAGVELARIKPVLHIQRGVPALPADWLELVSFVARYYHAPLGEVVALALPPG
                     LRRADGVSDEDEDPLLGVAPKGGEALCAARRASRALALLQAL"
     gene            complement(825..>1649)
                     /locus_tag="PKV97_17975"
     CDS             complement(825..>1649)
                     /locus_tag="PKV97_17975"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNB07829.1"
                     /translation="DAMGLGLYFAEGEGPRFERPLKDEWEIRNLSAPDPHAELQYVMD
                     AVAEIRRALDGSVPLIGFSGSPWTLACYMVEGGSSDDYRKVKSLAYSRPDLMHHILDV
                     TAQAVVKYLNAQIEAGAQAVMVFDSWGGVLSEAAYKEFSLPYLEQVVAGLIRERDGQR
                     VPSIVFTKGGGLWLESIAAIGCDAVGLDWTMDIGRARRLVGDKVALQGNLDPNVLFAP
                     PEAVATETRRVLDAFGNHPGHVFNLGHGISQYTPPESVSVLVDTVHAHSRAIRAGA"
BASE COUNT          293 a          559 c          540 g          257 t
ORIGIN      
        1 cgagcgcctg cagcagcgcg agcgcccggc tcgcccgccg cgccgcgcaa agcgcctcgc
       61 cgcccttcgg ggcgacgcca agcagcggat cctcgtcctc gtcactgacg ccgtcggcgc
      121 gacgcaggcc aggaggcagc gcgagcgcca ccacctcgcc gagcggcgcg tggtaatagc
      181 gcgccacgaa ggacaccagc tccagccagt ccgcaggcaa ggccggcacg ccgcgctgga
      241 tgtgcagcac gggcttgatc cgtgcgagtt ccacccccgc ttcgtcgccg ataccgacga
      301 tcaacccggt gcgttcgccg cgcccgaacg gcaccttcac gcagcgcccg acgtctccca
      361 cgcccgcatc cgcggccgtg taatcgaagg cttgcggcag cgggatcggc agggcgacgc
      421 ggacgatagc catggagcgg cggaggttgg agaaaaaatt tttcttcttc agcgacttgc
      481 accacttcgc tgagagcgga ccgagaaaac ggcgggcaag tggctggcgg gaaatgaggc
      541 ttccagcttg tccacaaaac ctgtggataa gtttgtggga aagctgggtg aatcgggctc
      601 aaacctgcgc cgcacaaggg gtttcgtcac aatgccttaa cattgcgcga atttttctat
      661 tttattttca atgacttatg aaagccatcg ggcaaagccc gttatgggcg gcattcccgg
      721 gcagaacccg tgaaatgtgc ataagtcaag cccggaaaac acgaattcgt gcttgacaga
      781 tggcggcgga ggcctgtcct gcagcgcacc gccgcccgca cgcctcaagc cccggcgcgg
      841 atcgcccggc tgtgcgcgtg cacggtgtct accagcacgc tcacgctctc cggaggggtg
      901 tactgcgaga tgccatggcc gagattgaag acgtgtcccg gatggttgcc gaaggcgtcg
      961 agcacccggc gcgtctccgt cgcgaccgcc tcgggcgggg cgaacaacac gttggggtcg
     1021 aggttgccct gcagcgccac cttgtcgccc accaggcgac gcgcgcggcc gatgtccatg
     1081 gtccagtcga ggccgaccgc gtcgcaaccg atcgcggcga tcgactccag ccacaggccg
     1141 ccgcccttgg tgaacacgat gctgggcacg cgctggccgt cgcgctcacg gatcaggcct
     1201 gctacgacct gttccaggta aggcagcgag aactccttgt acgcggcctc ggacagcaca
     1261 ccgccccagg agtcgaacac catcaccgcc tgcgcgccgg cctcgatctg cgcgttgagg
     1321 tacttcacca ccgcctgcgc ggtgacgtcg aggatgtggt gcatcaggtc ggggcggctg
     1381 taggccagcg acttgacctt gcggtagtcg tcggaggagc cgccctcgac catgtagcag
     1441 gccagggtcc acgggctgcc cgagaaaccg atcagcggca cgctgccgtc gagcgcacgg
     1501 cggatctcgg cgaccgcatc catcacgtac tgcagctcgg cgtgggggtc cggcgcgctg
     1561 aggttgcgga tctcccactc gtccttgagc gggcgctcga agcgcgggcc ctcgccctcg
     1621 gcgaagtaca ggccgaggcc catcgcgtc
//
LOCUS       DAOQNN010000618         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_68715_length_1648_cov_13.858757, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000618 DAOQNN010000000
VERSION     DAOQNN010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68715_length_1648_cov_13.858757"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..631)
                     /locus_tag="PKV97_17980"
     CDS             complement(26..631)
                     /locus_tag="PKV97_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HNB07830.1"
                     /translation="MSNVYLNGFLLTLGLIMAIGAQNAHVLRQGLRGEHVLLTIGVSA
                     ACDILLIGLGIAGVGSLFVANPDLMTVARYGGAAFLAWYGARALRSALRGGRALDLAE
                     HKRMSRGQALLAAAGFSLLNPHAYLDTVVLLGSIGSQQAEDLRPLFAAGAISASIVWF
                     LLLGYGARLLAPLFAKPVAWRVLDALVALMLWAIAGSLLLG"
     gene            810..>1648
                     /locus_tag="PKV97_17985"
     CDS             810..>1648
                     /locus_tag="PKV97_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HNB07831.1"
                     /translation="MIDHRLLAFEAVLQEGGFERAARRLALTQSAVSQRVKLLEAELG
                     QVLLVRSKPVRPTPAGRRLLPYLAQLRLMEAEARRALSPRQVDGPLRLAVGVNADSLA
                     TWFIGAVAEVVRDEGIVLDCVVDDQDHTHALLADGEVLGCVSTRADPMRGCAAVRLGA
                     MPYLCAGSPDFRALWFPQGLTATALARAPAIVFGHHDDMHEAFLLRNFGLDSRRYPHH
                     VVPSSEGFMAFALAGLGYGFVPEIQARAHLARGELVDLAPEREEVVLYWHHWQVQSPV
                     MARL"
BASE COUNT          248 a          569 c          583 g          248 t
ORIGIN      
        1 ggcgcgaatg ggcgctcacg cccgctcacc ccagcagcag cgagcccgcg atcgcccaca
       61 gcatcagcgc caccagcgca tcgagcacac gccatgccac cggcttggcg aacagcggcg
      121 cgagcaggcg ggcgccgtag ccgagcagca ggaaccacac gatcgaggcg ctgatcgcgc
      181 cggcggcgaa gagcgggcgc aggtcctcgg cctgctggct gccgatcgaa ccgagcagca
      241 ccacggtgtc gagataggca tgcgggttga gcaggctgaa gccggcggcc gcgagcaggg
      301 cctggccgcg cgacatgcgc ttgtgctcgg ccaggtccag cgcgcgccca ccgcgcagcg
      361 cggagcgtag cgcgcgcgcg ccgtaccagg cgaggaaggc cgccccaccg tagcgcgcca
      421 ccgtcatcag gtcggggttg gcgacgaaca ggctgcccac gccggcgata cccaggccga
      481 tcaacaggat gtcgcacgcc gccgacacgc cgatcgtcag cagcacgtgc tcgccgcgca
      541 ggccctggcg cagcacgtgc gcgttctgcg ccccgatcgc catgatcagg ccgagggtga
      601 ggagaaagcc gttgaggtag acgttgctca tgatgcgttc cgttgtcgtc tcgtgttgcc
      661 gtggccgcag ccctgttcgc gactgcgggc gctccggcaa gaaccggcat gaggcggacc
      721 tgcagaagcg ccgggaggcg cgaatatagg cgccaggcgg tcttttatta agtaggatcg
      781 atttatttcg ttttcgagca ataacgctaa tgatcgatca tcgcttgctg gccttcgagg
      841 cggtgctgca ggagggcgga ttcgagcgcg cggcgcgccg gctggcgctg acccagtccg
      901 cggtgtcgca gcgggtcaag ctgctcgagg ccgagctcgg ccaggttctg ctggtgcgca
      961 gcaagccggt gcggccgacg ccggccgggc gcaggctgct gccctacctt gcacagctgc
     1021 gcctgatgga ggcggaggcg cgacgcgcgc tgtcgccgcg ccaggtggac gggccactgc
     1081 gtctcgcagt gggcgtcaac gccgattcgc tcgccacctg gttcatcggc gcggtcgccg
     1141 aggtggtccg cgacgagggc atcgtgctcg actgcgtggt cgacgaccag gaccacaccc
     1201 acgcgctgct cgccgacggc gaggtgctgg gttgcgtgtc cacccgcgcc gatccgatgc
     1261 gcggctgcgc cgccgtacgc ctcggggcca tgccctatct gtgcgccggc tcgccggact
     1321 tccgcgcact ctggtttccg caggggctca cggcgacggc gctcgcacgt gccccggcga
     1381 tcgtcttcgg ccaccacgac gacatgcacg aggccttcct gctgcgcaac ttcgggctgg
     1441 attcgcggcg ctatccacac cacgtcgtgc cctcgtccga gggcttcatg gccttcgcgc
     1501 tcgccggcct gggctatgga ttcgtccccg agatccaggc acgtgcgcac cttgcgcgcg
     1561 gcgagctggt cgacctggca cccgagcgcg aagaggtggt cctgtactgg caccactggc
     1621 aggtacagtc tccggtgatg gcgaggct
//
LOCUS       DAOQNN010000619         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_68720_length_1648_cov_6.639674, whole genome shotgun sequence.
ACCESSION   DAOQNN010000619 DAOQNN010000000
VERSION     DAOQNN010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68720_length_1648_cov_6.639674"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            662..>1648
                     /locus_tag="PKV97_17990"
     CDS             662..>1648
                     /locus_tag="PKV97_17990"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HNB07832.1"
                     /translation="MSGGLHRGAEPTAALHADDFDFPAPRRRTRRRVEPPIALAPRAH
                     YTPRQRLLLLDTWLRSKLPAQDFGALVGLSIHTLYLWKRRFEELGPAGLENQRRGPQK
                     GSRLSLETQRAILLMKQSHPEWGEDRIHDMLARSQGLGASPGAIGHYLQQEGYVVEQI
                     PTRPHPERVQHFERSRPNELWQTDLFTFVLKRQNRRVHMVAFMDDHSRFIVGYGIHAS
                     SSGALVREVLEAAIANFGAPLEVLTDNGTQYHTWRGKSAFTALLERRGIKQIVARPRH
                     PQTLGKVERFWGTLWRECVERAVFLDLDDARRRIGHFIDHYNFQRTHSGIDGL"
BASE COUNT          251 a          630 c          527 g          240 t
ORIGIN      
        1 aggtcaggtt cgtccggctg atcctctcga actcctggtc cttcttctcc ggtgcgcacg
       61 cgcagaccct ccttggactg atcgccgagc aggcattgac tcgatccatg tgcccagggc
      121 gccgtggctc ggggcggggc gctcgggaga ggctcgggct gagggcgtcg ccaccttcca
      181 ctggcggggc cgtcagctcg accgacgcgc aatcatgctg tgcctcgcgg agatccgtca
      241 acggggccgg ggagccgtgg tctgacactc tggcacgggt ggtggaggct gtcgcgcggg
      301 aggcggcgca tccggatgcc cttccgcctg gagtcggagg aaggtcagtg gcgaacgcgg
      361 acgggcggaa ccgagggcac tcgccgaggg cctcgagccg cccggatccc ctggctttgg
      421 cccgagtgtg caacggtgtc cccctcgccg aaatcccagc cccgctcacc cgtgaatccc
      481 ctggatgaag tcatcggtcc ctacccgctc cgcactccgc ggagccaggt gacccatgac
      541 tgctccgtac gtacccgatc ccgctgaccc cgcagtaccg catcccacct tcagcacgca
      601 gcccccgcct tccgcgctgc agcacgacgc gagcgaggcc gagggcgtag cccgaggccg
      661 agtgagcggc gggctgcatc gcggcgccga gcccaccgcc gcgctgcacg ccgacgactt
      721 cgacttcccc gctccgcgcc gccgcacccg tcgtcgcgtc gagccgccga tcgcgctcgc
      781 gccgcgcgcg cactacacgc cccgccagcg cctgctgctc ctggacacct ggctgcgcag
      841 caagctcccc gcccaggact tcggcgccct ggtcggactc tcgatccaca ccctctacct
      901 gtggaagcgt cgcttcgagg agctcggtcc cgccggtctc gagaaccagc gtcgcgggcc
      961 gcagaagggc agccgtctct ccctcgagac gcagcgcgcc atcctgctga tgaagcagtc
     1021 gcaccccgag tggggcgagg atcgcatcca cgacatgctc gcgcgcagcc aggggctggg
     1081 cgccagcccg ggcgcgatcg gccactacct gcagcaggag ggctacgtgg tcgagcagat
     1141 cccgaccaga ccgcatcccg agcgcgtcca gcacttcgag cgcagccgcc ccaacgagct
     1201 gtggcagacc gacctcttca ccttcgtcct caagcggcag aaccggcgcg tgcacatggt
     1261 cgccttcatg gacgaccaca gccgtttcat cgtcggctac ggcatccacg ccagcagctc
     1321 gggtgctctg gtgcgcgagg tcctcgaggc ggcgatcgcc aacttcgggg ccccgctcga
     1381 ggtcctcacc gacaacggca cccagtacca cacctggcgc ggcaagagcg ccttcaccgc
     1441 cctgctcgag cgccgcggca tcaagcagat cgtcgcgcgc ccgcgccacc cgcagacgct
     1501 gggcaaggtc gagcgcttct ggggcacctt gtggcgcgag tgcgtcgagc gcgccgtctt
     1561 cctcgacctc gacgacgcaa ggcgccgcat cgggcacttc atcgaccact acaacttcca
     1621 gcgcacccat tcgggcatcg acggactg
//
LOCUS       DAOQNN010000620         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_68894_length_1646_cov_11.517913, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000620 DAOQNN010000000
VERSION     DAOQNN010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68894_length_1646_cov_11.517913"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKV97_17995"
     CDS             <1..212
                     /locus_tag="PKV97_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07833.1"
                     /translation="RARGLAALMDTQDRDLIVARLIVLARRCVEAGDDPRHQSADALR
                     AAARDAQAAGLSVADILAATNYDAP"
     gene            291..962
                     /locus_tag="PKV97_18000"
     CDS             291..962
                     /locus_tag="PKV97_18000"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07834.1"
                     /translation="MDNTTIGKGASISEDASIGKDATIGKGAEIGKGAEIGKYARIGE
                     GATIGKGATIGEGAEIGKYARIGEGATIGEGAEIGKYARIGKGASIGKDASIGEGASI
                     GKYARIGEGASIGKDASISEDASIGEGASIGECGIVGTGARVMPHTHWGVSPYYVICG
                     RWPAYDAGDGHFGVGCQVHPISHWIEHLDWIASAKGATDLERELARLALHTVCTLRGI
                     EVPTT"
     gene            complement(967..1590)
                     /locus_tag="PKV97_18005"
     CDS             complement(967..1590)
                     /locus_tag="PKV97_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07835.1"
                     /translation="MNDRVQRLERKGHDVQRVEGCMRYAVATNDPLALIKGAVSWELN
                     DESLHAFGDRILHVPVIGHWSRAGVDPTDLVEGDALARHVLWLAALAAEAIPEDRFWV
                     RAGDQQLARLAAVSSGLRGDLLVRLWAEVAAGGSSWCLLGEVHGAALVDPAGLLGGLY
                     PAPRGGLRIVAEALRGLSDGVERRRGAHRWHAVPLDLIRAYAARCGL"
BASE COUNT          265 a          575 c          614 g          192 t
ORIGIN      
        1 gccgagcgcg ggggctcgcg gcgttgatgg acacccaaga tcgagatctg atcgttgctc
       61 gtctgatcgt gctcgcccgg cggtgcgtcg aggccggcga cgaccctcgg caccagtcgg
      121 ccgacgcgct ccgggccgcg gcacgggacg cacaagctgc tggtctgtcc gtcgcggaca
      181 tcctggccgc gaccaactac gacgccccgt agaccacccg cccccgcgag cccccgcccc
      241 tggcgcagca cgcgagtgtc gagcgcgggg gctcgcggca ttggaggacc atggacaaca
      301 cgaccatcgg caagggcgcg agcatcagcg aggacgcgag catcggcaag gacgcgacca
      361 tcggcaaggg cgcggagatc ggcaagggcg cggagatcgg caagtacgcg cgcatcggcg
      421 agggcgcgac catcggcaag ggcgcgacca tcggcgaggg cgcggagatc ggcaagtacg
      481 cgcgcatcgg cgagggcgcg accatcggcg agggcgcgga gatcggcaag tacgcgcgca
      541 tcggcaaggg cgcgagcatc ggcaaggacg cgagcatcgg cgagggcgcg agcatcggca
      601 agtacgcgcg catcggcgag ggcgcgagca tcggcaagga cgcgagcatc agcgaggacg
      661 cgagcatcgg cgagggcgcg agcatcggcg agtgtgggat cgttgggacg ggcgcgcggg
      721 tgatgccgca cacacattgg ggcgtgtcgc cgtactacgt gatctgcggc cgctggccgg
      781 cgtacgatgc cggtgacggg catttcggcg tggggtgcca ggtgcatccg atctcgcact
      841 ggatcgagca cttggactgg atcgcgtcgg ccaaaggtgc gaccgatctc gagcgggagc
      901 tggctcggct cgcgctgcac acggtctgca cgctgcgcgg gatcgaggtg ccgacgacct
      961 gagaggctag agcccgcagc gcgccgcgta cgcgcgaatc aggtcgagcg ggacggcgtg
     1021 ccagcgatgc gcgccgcgtc gccgctcgac gccgtcggac agcccgcgca gcgcttcggc
     1081 gacgatccgc aggccgccgc gcggcgctgg gtatagcccg ccgagcagtc ccgcggggtc
     1141 gaccagcgcc gcgccgtgca cctcgccgag caggcaccag ctcgagccgc cggccgcgac
     1201 ctcggcccac agccggacga gcagatcgcc gcgcagcccc gacgagaccg cggcgagccg
     1261 cgcgagctgc tgatcgccgg cgcggaccca gaaccgatcc tcggggatcg cctcggccgc
     1321 gagcgcggct agccacagca cgtgccgcgc gagtgcgtcg ccctcgacga ggtcggtggg
     1381 gtcgaccccg gcgcgcgacc agtgcccgat caccggcacg tgcaggatcc gatcgccgaa
     1441 cgcgtgcagc gattcgtcgt tcagctccca ggacaccgcg cccttgatca acgccaacgg
     1501 atcgttcgtc gcgacggcgt agcgcatgca gccttcgacg cgctgcacgt cgtggccctt
     1561 gcgctcgagc cgctgcaccc gatcgttcac gccctcgcgc aggaaccccg ccagcgccgc
     1621 gccggtctca gtgctgctcg tgtcgt
//
LOCUS       DAOQNN010000621         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_68967_length_1645_cov_10.349057, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000621 DAOQNN010000000
VERSION     DAOQNN010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68967_length_1645_cov_10.349057"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="PKV97_18010"
     CDS             complement(<1..1232)
                     /locus_tag="PKV97_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNB07836.1"
                     /translation="MTTTPRNVLPKGVSANDFEQALAQIRAIVGAEYVVAEDTALAPY
                     NKIMMAVPDEQHAPSAAVMPDSVEQIQAIMKVLNQHRIPVYPISTGKNLGYGSAAPVE
                     RGQLVMDLRRMNRILDVDAELCTALVEPGVTYQQLYDYLQEKKLPLWFSCPAPSVIAG
                     PLGNMVDRGVGYTPYGEHFLFSSGMEVVLADGEVLRTGMGSMPGSNTWQVFKWGYGPT
                     LDGIFTQSNYGIVTKMGMWLMPAPPDYRPFLIQYPEDTDITKIVDALRPLRIAMVIPN
                     AVVIAHTLWEAPCTKVNRADYHPGPGAISDEAVQKIRKDHNLGAWNVYAALYGTPETN
                     AANWKIIEQVAAATGGTLITDSTEQGGRATQYRFDLMKGKPNLGEFDLYNWRGGGGSM
                     WFAPVSQAKGSETLKQMQL"
     gene            complement(1288..>1645)
                     /locus_tag="PKV97_18015"
     CDS             complement(1288..>1645)
                     /locus_tag="PKV97_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07837.1"
                     /translation="LRRPEQVDALLALPHGTRLLGVMDDASAIVFLAMASSRGAHLLA
                     HGHHRLASQGATRHACSATGCGEALAWHEPASHPATHVERFYLATLGETSPGARTSLP
                     AVPGGDEALASFLIRL"
BASE COUNT          225 a          506 c          632 g          282 t
ORIGIN      
        1 agctgcatct gcttgagcgt ctcgctgccc ttggcctgcg acaccggcgc gaaccacatc
       61 gagccgccgc ccccgcgcca gttgtagagg tcgaattcgc cgaggttggg cttgcccttc
      121 atgaggtcga agcggtactg ggtggcgcgc ccgccctgtt cggtcgagtc ggtgatcagc
      181 gtgccgccgg tggccgcggc cacctgctcg atgatcttcc agttcgccgc gttggtctcc
      241 ggcgtgccgt agagcgcggc atacacgttc caggcgccga ggttgtggtc cttgcggatc
      301 ttctgcaccg cctcgtcgct gatcgcgccc gggcccgggt ggtagtcggc gcgattgacc
      361 ttggtgcagg gcgcctccca cagcgtgtgg gcgatcacca ccgcgttggg gatcaccatc
      421 gcgatgcgca gcgggcgcag cgcgtcgacg atcttggtga tgtcggtgtc ttccgggtac
      481 tggatcagga agggacggta gtcgggcggc gccggcatca gccacatgcc catcttggtg
      541 acgatgccgt agttcgactg ggtgaagatg ccgtccagcg tcgggccgta gccccacttg
      601 aagacctgcc aggtgttcga gcctggcatc gagcccatgc cggtgcgcag cacctcgccg
      661 tcggcgagca ccacctccat gcccgacgag aacaggaagt gctcgccgta gggcgtgtag
      721 cccacgccgc ggtcgaccat gttgccgagc gggccggcga tcacggaagg ggcggggcag
      781 gagaaccaca gcggcagctt cttctcctgc aggtagtcgt agagctgctg gtaggtcacg
      841 ccgggctcga cgagcgcggt gcacagctcg gcatcgacgt cgaggatgcg gttcatgcgg
      901 cgcaggtcca tgaccagctg gccgcgctcg accggggcgg ccgagccgta gcccaggttc
      961 ttgccggtgg agatcggata caccgggatg cggtgctggt tgagcacctt catgatggcc
     1021 tggatctgct cgacgctgtc gggcatcacc gcggcggacg gcgcgtgctg ctcgtcgggc
     1081 acggccatca tgatcttgtt gtagggggcg agggcggtgt cctcggccac gacgtattcg
     1141 gcgccgacga tcgcgcggat ctgggcgagg gcctgttcga agtcgttcgc actgacgccc
     1201 ttgggcagca cgttgcgcgg ggtggtggtc atggtttctc cgggtgcgga aggcggtggg
     1261 atggcgttcg ggtggtggtc cggagggtca gagccggatc aggaaggagg cgagcgcttc
     1321 atcgccgccc gggactgcgg gcagcgacgt gcgtgcgccc ggggaggttt cgccgagtgt
     1381 ggcgaggtag aagcgctcga cgtgcgtggc cggatgcgag gccggttcgt gccaggcgag
     1441 cgcctcgccg cagccggtgg cgctgcaggc gtggcgggtc gcgccctgcg aggcgaggcg
     1501 atggtggccg tgggcgagca ggtgcgcgcc gcggctcgac gccatcgcca ggaagacgat
     1561 cgccgaggcg tcgtccatca cgccgaggag gcgggtgccg tgcggcagcg cgagcagcgc
     1621 gtcgacctgc tcggggcggc gcagc
//
LOCUS       DAOQNN010000622         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_69635_length_1637_cov_8.441846, whole genome shotgun sequence.
ACCESSION   DAOQNN010000622 DAOQNN010000000
VERSION     DAOQNN010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69635_length_1637_cov_8.441846"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1637
                     /locus_tag="PKV97_18020"
     CDS             <1..>1637
                     /locus_tag="PKV97_18020"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB07838.1"
                     /translation="HAREAHLRIWGEVVEGLHEGIVVTNLAGDILDCNPAFERITGHA
                     RAEVLGLNPRVLKSGRHDAAFYAGMWDALVTRGSWVGEVWNRRKSGEIYPERLALRAV
                     RGADGLPTHYVGMFSDISREKQDQARLDYLTHHDPLTGFANRQLFMERLQQAVERAAA
                     GEGKAAVLLLDLDRFRRVNESLGQDAGDRLLQVMAERIAGCLKRRSSLARLGSDEFLV
                     LITEFTDADALAALARRILDEIARPVTVNGQLLHMSASLGISVHPQDGADAEHLLQAA
                     EAARAQIRHGLGNRLHFFTAGMDTRARRWIEVESELRHALARGELQLYYQPIVCVRDG
                     RVRTVEALLRWNSPALGWVSPAEFIPVAEESELIVAIGDWALETACRQVRHWQDTGLP
                     AVRVAVNVSAHQLAGGRLPERVREVLERTGIDPGQLELELTESAMMSDSEHGARQVEA
                     ISRMGVSVALDDFGTGYSSLAYLSRFALTKLKIDRSFVDGLSRDPKSSIIVDATLGLA
                     RGLGLRVVAEGVETEEQRALLAAAGCDALQGYLFSPP"
BASE COUNT          240 a          597 c          581 g          219 t
ORIGIN      
        1 gcacgcccgc gaggcccacc tgcgcatctg gggcgaggtc gtcgagggcc tgcacgaagg
       61 catcgtggtg accaacctcg ccggcgacat cctcgactgc aacccggcct tcgagcgcat
      121 caccggccat gcgcgcgccg aggtgctcgg gctcaatccg cgcgtgctca aatccggccg
      181 ccacgacgcc gccttctacg ccggcatgtg ggacgcactg gtcacgcgcg gctcgtgggt
      241 cggcgaggtg tggaaccggc gcaagagcgg cgagatctac cccgagcgcc tggcattgcg
      301 tgcggtgcgc ggcgccgacg gcctgcccac gcactacgtg ggcatgttca gcgacatcag
      361 ccgcgagaag caggaccagg cccggctcga ctacctgacc caccacgatc ccctcaccgg
      421 cttcgccaac cgccagctct tcatggagcg gctgcagcag gcggtggagc gcgccgccgc
      481 cggcgagggc aaggccgcgg tgctgctgct cgacctcgac cgcttccggc gggtgaacga
      541 gagcctcggt caggacgccg gcgaccgcct gctgcaagtg atggccgagc gcatcgccgg
      601 ctgcctgaag cggcgcagca gcctcgcccg cctgggtagt gacgagttcc tcgtgctgat
      661 caccgagttc accgatgccg acgcgctggc cgcgctcgcg cgacgcatcc tcgacgagat
      721 cgcccgcccg gtgacggtca acggccagct cctgcacatg agcgcgagcc tgggcatcag
      781 cgtccatcct caggacggcg ccgacgccga gcacctgctg caggcggccg aggccgcgcg
      841 tgcgcagatc cgccacggcc tgggcaaccg cctgcacttc ttcaccgccg gcatggacac
      901 ccgggcacgg cgctggatcg aggtcgagag cgagctgcgc catgcgctcg cgcgcggcga
      961 gctgcagctc tactaccagc cgatcgtgtg cgtgcgcgat ggccgcgtac gcaccgtgga
     1021 ggcgctgctg cgctggaaca gccccgcgct gggctgggtg tcgccggcgg agttcatccc
     1081 ggtggccgag gagagcgagc tgatcgtcgc gatcggcgac tgggcgctcg agaccgcctg
     1141 ccgccaggtc cgccattggc aggacaccgg cctgccggcg gtgcgcgtgg cggtgaacgt
     1201 ctccgcgcac cagctcgcgg gcgggcgcct gcccgagcgc gtgcgcgagg tgctcgagcg
     1261 caccggcatc gaccccggcc agctcgagct cgagctcacc gagagcgcga tgatgagcga
     1321 cagcgagcac ggcgcgcgcc aggtcgaggc gatcagccgc atgggggtga gcgtcgcgct
     1381 cgacgacttc ggcaccggct actcctcgct cgcctacctg agccgcttcg ccctcaccaa
     1441 gctgaagatc gaccgcagct tcgtcgatgg cctgagccgc gacccgaaga gctcgatcat
     1501 cgtggatgca accctgggcc tggcgcgcgg cctgggcctg cgcgtggtcg ccgagggcgt
     1561 ggagacggag gagcagcgcg cgctgctcgc cgccgccggc tgcgacgcgc tgcaaggcta
     1621 tctgttcagc ccgccgg
//
LOCUS       DAOQNN010000623         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_69639_length_1637_cov_5.642857, whole genome shotgun sequence.
ACCESSION   DAOQNN010000623 DAOQNN010000000
VERSION     DAOQNN010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69639_length_1637_cov_5.642857"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1554
                     /locus_tag="PKV97_18025"
     CDS             70..1554
                     /locus_tag="PKV97_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07839.1"
                     /translation="MGWFSAGKGAAEPVPAPVVEETPKREARPRGERSGEGRRNGGSN
                     GGRGRRGERDGEAQPSTRGSRAERGESTERPQRVAAERAERPERAERPERGERAERSE
                     GGRRGRGQRGERAQEGAEQRAAQTEDDLLKAATATAMVAAATTADSGTTETVASTEGG
                     EEEGTPSEKRRRRGRGRGRRSGEPQGIDGAAEAGNEVETGATAPEGELSGIAASAVEQ
                     GTVESAHAASPVTAGQDANVGAAATAVVQTAPADAQIAELPIVGGTAEDVTPVALAAP
                     QVPLAPVPVGAALEVVAETAPAAEVPVDKALAAEAVLAEQGAAAEELAAGSAAMVEAV
                     SAAAPRAETAAMIDSASAEEAPPAVASAAGEEAVIGTEPVAVAEPAPTSSVGVPPEVA
                     AAAPEAVLAPLADSTEVAEPSVPAAAESTVVRAMEAGRASHAPSAPIAVPSGDSGLIR
                     IETDPAKRVQFGTGAPEQPARLGRQPRPAAVIAEEPLQQVETRK"
BASE COUNT          244 a          616 c          614 g          163 t
ORIGIN      
        1 cccgagcccg ctcccgcctc ggccccggcc cctgccgcgc ccgagcccaa ggggctgatc
       61 gcgcgcatca tgggctggtt ctccgccggc aagggcgccg cggaaccggt ccccgccccg
      121 gtcgtggaag agactccgaa acgcgaggcg cgcccgcgtg gcgaacgcag cggcgagggt
      181 cgccgcaatg gcggcagcaa tggcggccgt ggccgtcgcg gcgagcgcga cggcgaggcg
      241 cagccctcca cccgcggcag ccgtgccgag cgtggcgaga gcaccgagcg tccgcagcgc
      301 gtcgcggccg agcgtgccga gcgccccgag cgtgccgagc gccccgagcg tggtgagcga
      361 gccgagcgca gcgaaggcgg ccgccgtggt cgcggccagc gcggcgagcg tgcccaggaa
      421 ggcgccgagc agcgcgcggc ccagaccgag gacgatctgc tgaaggccgc caccgccact
      481 gcgatggtgg cagcggccac gacggcggac tccggcacga ccgagaccgt tgccagcacc
      541 gaaggcggcg aagaggaagg caccccgtcc gagaagcgcc gccgtcgcgg ccgtggccgc
      601 ggccgtcgct cgggcgagcc gcagggcatc gacggcgccg ccgaagcggg taacgaagtc
      661 gagaccgggg cgacagcgcc cgagggcgaa ctgtcgggaa tcgcggcgtc cgccgtcgag
      721 caggggaccg tcgagtccgc gcacgctgcc agcccggtca cggccggaca ggacgcgaac
      781 gtcggcgctg ccgccacagc cgtggtgcaa accgctccgg cggatgcgca gatcgccgaa
      841 ctgccgatcg tcggcggcac cgcggaggac gtcacaccgg tcgccctggc cgcacctcag
      901 gtgccgcttg ccccggtgcc ggtcggcgca gcgctggaag tcgtcgctga aacggcgcct
      961 gccgcggagg tcccggtcga caaggccctt gcggccgaag cggtgctcgc cgagcaaggt
     1021 gctgctgccg aagagcttgc cgccgggagt gctgccatgg tcgaagcggt atccgccgcg
     1081 gcgccccgtg ccgagaccgc tgccatgatc gattcggctt ctgccgagga agcgccgcct
     1141 gcggtcgcct ccgcagcagg cgaggaggcg gtcatcggca ccgaacccgt cgcggtcgcc
     1201 gagcccgccc ccacgtcgag cgtcggcgtc ccccccgagg tcgccgccgc agcgccggaa
     1261 gccgtgctag ccccgctcgc ggattcgacg gaggtcgccg aaccgtccgt gcctgcggca
     1321 gccgagtcga cggtggttcg cgcgatggaa gccggccgtg ccagccacgc gccgagcgca
     1381 ccgatcgcgg tgccgtccgg cgacagcggc ctgatcagga tcgagaccga tccggccaag
     1441 cgcgtgcagt tcggcaccgg cgccccggag cagccggcgc gcctgggccg ccagccccgc
     1501 ccggccgcag tgatcgccga ggagccgctg caacaggtcg agacccgcaa gtaagcggtt
     1561 ccaccaaccc cggcgcgaag gcttgtcgag cgcacatcgc gacttgcatc gctcctgcag
     1621 gaaaccagcc cgcgtgg
//
LOCUS       DAOQNN010000624         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_70176_length_1630_cov_13.326349, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000624 DAOQNN010000000
VERSION     DAOQNN010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70176_length_1630_cov_13.326349"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1630
                     /locus_tag="PKV97_18030"
     CDS             <1..>1630
                     /locus_tag="PKV97_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944786.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNB07840.1"
                     /translation="KNKVRFVTAAALFDGHDASINIMRRILQSTGSEVIHLGHNRSVG
                     EIVNAALQEDVQGIAITSYQGGHVEFFKYMIDLLRANGGENIKVFGGGGGVIVPAEIK
                     ELHEYGVTHIFSPEDGAKMGLQGMINSVVERCDVDVAKAAPQKADAVLKALAAGDRRA
                     LAQIITALENGGYGDEVKKALIEAAAKTKVPTLGITGTGGAGKSSLTDELVRRFRLDQ
                     DDKLKLAIVSIDPSRKRTGGALLGDRIRMNAIEHANIYMRSLATRDTGSEVSAALPEV
                     IAACKLAGFDLVIVETSGIGQGNAAIVPFVDLSLYVMTPEFGAASQLEKIDMLDFADF
                     VAINKFDRKGALDALRDVAKQYQRNRELFNQRPEEMPVFGTMAARFNDDGVTALYQAV
                     LPALVGKGLKVAKSKLPVVKVCASSEGRAIVPADRTRYLAEIADTVRGYHKHIEQQAR
                     VARERQSLKIAKGLFEQCGKDAGSFAELIDWKDGELTPAAKKLLDMWPKTRELYAADE
                     YVVKIRDKEIRTPLTSESLSGSKIRKVALPSFEDD"
BASE COUNT          302 a          583 c          511 g          234 t
ORIGIN      
        1 aagaacaagg tgcgtttcgt caccgctgcc gcgctgttcg acggccatga cgcgtccatc
       61 aacatcatgc gccgcatcct gcagtcgacc ggctccgagg tcatccacct cggtcacaac
      121 cgctcggtcg gcgagatcgt caatgcggcg ctgcaggagg acgtgcaggg catcgcgatc
      181 acctcctacc agggtggtca cgtcgagttc ttcaagtaca tgatcgacct gctgcgcgcc
      241 aacggcggcg agaacatcaa ggtcttcggc ggcggtggcg gcgtgatcgt gccggctgag
      301 atcaaggaac tccacgagta cggcgtcacc cacatcttct cgcccgaaga cggcgccaag
      361 atgggccttc agggcatgat caacagcgtc gtcgagcgct gcgacgtcga cgtcgccaag
      421 gccgcgccgc agaaggccga tgcggtgctc aaggcgctcg ccgccggcga tcgccgcgcg
      481 ctcgcgcaga tcatcaccgc gctggagaac ggcggctacg gcgacgaggt caagaaggcg
      541 ctgatcgagg ccgcggccaa gaccaaggtg ccgacgctcg gcatcaccgg caccggcggc
      601 gcgggcaagt cctcgctgac cgacgagctg gtgcgccgct tccgcctcga ccaggacgac
      661 aagctcaagc tcgccatcgt ctccatcgac ccctcgcgca agcgcaccgg cggcgcgctg
      721 ctcggcgacc gcatccgcat gaacgcgatc gagcacgcca acatctacat gcgctcgctc
      781 gccacccgcg acaccggctc ggaagtctcc gccgcgctgc ccgaagtcat cgccgcgtgc
      841 aagctcgcgg gcttcgacct cgtcatcgtc gagacctccg gcatcggcca gggcaacgcc
      901 gcgatcgtgc ccttcgtcga tctgtcgctg tacgtcatga ccccggagtt cggtgccgcc
      961 agccagctcg agaagatcga catgctcgac ttcgccgact tcgtcgccat caacaagttc
     1021 gaccgcaagg gcgcgctgga cgcgctgcgc gacgttgcca agcagtacca gcgcaaccgc
     1081 gagctgttca accagcgccc cgaagagatg ccggtgttcg gcaccatggc cgcgcgcttc
     1141 aacgacgacg gcgtcaccgc gctctatcag gccgtgctgc cggcgctcgt cggcaagggc
     1201 ctcaaggtcg ccaagagcaa gctgccggtg gtcaaggtgt gcgcctcgtc cgaaggtcgc
     1261 gccatcgtcc ccgccgaccg cacgcgctac ctcgccgaga tcgccgacac cgtgcgcggc
     1321 taccacaagc acatcgagca gcaggctcgc gtcgcgcgcg agcgccagtc gctgaagatc
     1381 gccaagggcc tcttcgagca gtgcggcaag gacgcgggct cgttcgcgga actcatcgac
     1441 tggaaggacg gcgagctcac cccggccgcg aagaagctgc tcgacatgtg gccgaagacg
     1501 cgcgagctct acgccgccga cgagtacgtg gtgaagatcc gcgacaagga gatccgcacc
     1561 ccgctcacct ccgaatccct gtccggcagc aagatccgca aggtggccct gcccagcttc
     1621 gaggacgacg
//
LOCUS       DAOQNN010000625         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_70642_length_1624_cov_9.275972, whole genome shotgun sequence.
ACCESSION   DAOQNN010000625 DAOQNN010000000
VERSION     DAOQNN010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70642_length_1624_cov_9.275972"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..1411
                     /locus_tag="PKV97_18035"
     CDS             371..1411
                     /locus_tag="PKV97_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNB07841.1"
                     /translation="MHRLEQLRTWLAATLPGQSFELAPASADASFRRYFRATFADGSP
                     SRVVMDAPPEKEDVRPWLHVAGLFGAAGAHVPEVLAQDLDQGFLLLSDLGSTTYLQAL
                     KAHDDPQQAARTAHHLYADALGALAAIQCASRPSVLPEYDRALLLRELQLFPEWYIAR
                     HKGVTLTEAETAMLNTAFERILAVNLAEPQVFVHRDYHSRNLMLLEPAEGRGANPGII
                     DFQDAVYGPISYDLVSLFKDAYIRWDEEFILDLLIRYWETAKKLGLPVRAEFADFHRD
                     FEWMGVQRHIKVLGIFARLYHRDGKDGYLADMPLVMDYLRRACKRWRDLGPLLKLLDR
                     LEPENVQVGYTF"
     gene            1429..>1624
                     /locus_tag="PKV97_18040"
     CDS             1429..>1624
                     /locus_tag="PKV97_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNB07842.1"
                     /translation="MILAAGRGERMRPLTDHTPKPLLSVGGKPLIVWHIERLRASGFR
                     ELVINHAHLGHRLEEALGDGT"
BASE COUNT          263 a          612 c          521 g          228 t
ORIGIN      
        1 cggcaggctg tggcgtcggc gcagccaccg gcgccggacc gggagcggga actggagcga
       61 gagcgggaac ggacatgggc tccggagcgg gcgccttcga ggccggcgca gcggaggccg
      121 gggtcacggc gcgcgcaggc gtggccgtcg gggccggacg cagcagatcg ggcgccacct
      181 ggagcgtcgg cagggattgc gcgaaagcgg cacccgacag gcccagcaga agcagcggaa
      241 tcagacgaat gcgcgtagtc ggaagcatgc ggatcgagcc cttgccgtcg ttcgcgatgc
      301 gcgtgcgccc acccgaacca ccgtattgat aaactcgcgg attctacacg aacccctgcg
      361 gagatccggt ttgcatcgcc tagaacaact caggacctgg ctcgccgcca ccctgcccgg
      421 ccagtccttc gaactcgccc cggcctcggc ggacgccagc ttccgccgct acttccgcgc
      481 caccttcgcc gacggcagcc cctcgcgcgt ggtcatggac gccccgcccg aaaaggagga
      541 cgtacgcccc tggctgcacg tggccgggct cttcggcgcc gccggcgccc atgtgcccga
      601 ggtgctcgcg caggacctcg accaaggctt cctgctgctc tccgacctcg gctcgacgac
      661 ctacctgcaa gcgctgaagg cgcacgacga tccgcagcaa gccgcccgga ccgcacatca
      721 cctgtacgcg gacgcgctcg gcgcgctcgc cgccatccag tgcgccagcc gccccagcgt
      781 gctgcccgag tacgatcgcg cgctgctgct gcgcgagctg cagctcttcc ccgagtggta
      841 catcgcccgc cacaagggcg tcaccctcac cgaggccgag accgcgatgc tgaacaccgc
      901 gttcgagcgc atcctcgcgg tgaacctcgc cgagccgcag gtcttcgtgc atcgcgacta
      961 ccactcgcgc aacctgatgc tgctcgagcc cgccgagggc cgcggcgcca accccggcat
     1021 catcgacttc caggacgccg tctacggccc gatcagctac gacctcgtgt cgctgttcaa
     1081 ggacgcctac atccgctggg acgaggagtt catcctcgac ctgctgatcc gctactggga
     1141 gaccgcgaag aagctcggcc tgccggtgcg cgcggagttc gccgacttcc atcgcgactt
     1201 cgagtggatg ggcgtgcagc gccacatcaa ggtgctcggc atcttcgccc gcctgtacca
     1261 ccgcgacggc aaggacggct acctcgccga catgccgctg gtgatggact acctgcgccg
     1321 ggcctgcaag cgctggcgcg acctcggccc gctgctcaag ctgctcgatc gcctcgagcc
     1381 cgagaacgtc caggtcggct acaccttctg atggcacaga tccgcgcgat gatcctcgcc
     1441 gccggtcgtg gcgagcgcat gcgcccgctc accgaccaca cccccaagcc gctgctgtcg
     1501 gtgggcggca agcccctgat cgtatggcac atcgagcgcc tgcgcgcgtc cggcttccgc
     1561 gagctcgtga tcaaccacgc ccacctcggc caccggctcg aagaagcgct cggcgacggc
     1621 acgg
//
LOCUS       DAOQNN010000626         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_70884_length_1621_cov_7.991060, whole genome shotgun sequence.
ACCESSION   DAOQNN010000626 DAOQNN010000000
VERSION     DAOQNN010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70884_length_1621_cov_7.991060"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..>1621)
                     /locus_tag="PKV97_18045"
     CDS             complement(119..>1621)
                     /locus_tag="PKV97_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HNB07843.1"
                     /translation="TWRENVAYLHRDCAIYRAEGFQALSKVEVRANGRRILATLNVVD
                     DGCIVEPEELGVSIDAFFQLGVDDGHPAMIAQAEPPSSIPALHRKIAGERLAREDFRA
                     IVRDIAEHRYSKIELTAFVVATNQGELDREEVSFLTEAMAAVGDRIDWHERPVVDKHC
                     IGGIPGNRTSMLVVPIVAAHGMLCPKTSSRAITSPAGTADTMEVLARVELPTEELSGI
                     VREHRGCLAWGGTANLSPADDVLISVERPLSIDSPGQMVASILSKKLAAGSTHLVLDI
                     PVGPTAKVRSMAEAQKLRKLFEFVAGRMNLTIDVVITDGRQPIGSGIGPVLEARDVMR
                     VLENDPRAPYDLRQKALRLAGRMLEFDPDVRGGDGFAIARDILDSGRALAKMDAIIRA
                     QGARAFDHNAPQLARLSFEVCAEADGVVVAIDNHQLARIARLAGAPKMQGSGVDLLRK
                     LGEPVGRGEPLYRVHADYPADLEFARQASVRASGYALGTADGVPHLFVEF"
BASE COUNT          249 a          559 c          579 g          234 t
ORIGIN      
        1 gaagattaca cgccgtgcgg cggcggtcgc cgcgcagaaa ttcacgcccc ggctgcaaaa
       61 cggaacgcgg tccgcggtcc gcccggaagc ctgtcacgtc gcctcggcaa cgtcgagttc
      121 agaactccac gaacagatgc ggcacaccat ccgccgtccc cagcgcgtag ccgctggcgc
      181 gcacgctggc ctggcgggcg aactcgagat cggccggata gtcggcgtgg acgcggtaga
      241 gaggctcgcc cctgcccacc ggctcgccga gcttcctgag cagatcgacg cccgagccct
      301 gcatcttggg cgcccccgcc aggcgggcga tgcgcgcgag ctggtggttg tcgatcgcca
      361 ccaccacacc gtcggcctcg gcgcacacct cgaagctcag gcgggcgagc tgcggcgcgt
      421 tgtggtcgaa ggcgcgcgcg ccctgggcgc ggatgatcgc gtccatcttc gccagcgcgc
      481 gacccgagtc gaggatgtcg cgcgcgatcg cgaagccgtc gccgccgcgc acgtcggggt
      541 cgaactcgag catgcgcccg gccagacgca aggccttctg gcgcaggtcg tagggcgcgc
      601 gcgggtcgtt ctcgagcacg cgcatcacat cgcgcgcctc cagcaccggc ccgatcccgc
      661 tgccgatcgg ctggcggcca tcggtgatga ccacgtcgat ggtcaggttc atgcgcccgg
      721 cgacgaactc gaagagcttg cgcagcttct gcgcctcggc catcgagcgc accttggccg
      781 tcggtcccac cgggatgtcg agtaccaggt gggtcgaacc cgcggcgagc ttcttggaca
      841 ggatggaggc caccatctgc cccggcgagt cgatcgacag cggacgctcg accgagatca
      901 gcacgtcgtc ggccggcgac aggttggcgg tgccgcccca ggcgaggcag ccgcgatgct
      961 cgcgcacgat gcccgacagc tcctcggtcg gcagctcgac gcgcgcgagc acctccatgg
     1021 tgtcggcggt ccccgcgggc gaagtgatcg cgcgcgagga ggtcttgggg cacagcatgc
     1081 cgtgcgcggc gacgatcggc accaccagca tcgaggtgcg gttgccgggg atgccgccga
     1141 tgcaatgctt gtccaccacc gggcgctcgt gccagtcgat gcggtcgccc accgccgcca
     1201 tcgcctcggt gaggaaagag acctcctcgc gatcgagttc gccctggttg gtggcgacca
     1261 cgaaggcggt gagctcgatc ttggaatagc ggtgctcggc gatgtcgcgg acgatggcgc
     1321 ggaagtcctc acgggcgagc cgctcgccgg cgatcttgcg atgcagcgcc gggatggagg
     1381 acggcggctc ggcctgcgcg atcatcgccg gatggccgtc gtcgacgccg agctggaaga
     1441 aggcgtcgat cgacaccccg agctcttcgg gctcgacgat gcagccgtcg tcgacgacgt
     1501 tgagggtggc gaggatgcgg cgcccgttgg cccgcacctc gaccttggaa agggcctgga
     1561 aaccctcggc gcgatagatc gcgcagtcgc ggtgcaggta ggcgacgttc tcgcgccagg
     1621 t
//
LOCUS       DAOQNN010000627         1613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_71527_length_1613_cov_10.297176, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000627 DAOQNN010000000
VERSION     DAOQNN010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71527_length_1613_cov_10.297176"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="PKV97_18050"
     CDS             <1..1601
                     /locus_tag="PKV97_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB07844.1"
                     /translation="HRGIAYGLVAPLFEQGKVCANHLANFGIGRYEGSVTSTKLKVTG
                     IDVFSAGDFSGGPGTEDIVLHDRQGGVYKRVVLKDDRIVGSVLYGDTADGSWYFQLMK
                     DRQSVAEIRDHLMFGQNKLGDAGHKGENRAASMPDTAEVCGCNGVCKGTIVKAIKEKG
                     LFTLDEVKKHTKAASSCGSCTGLVEQILASTVGGAYQAVSANDKPVCACTEHSHKVVR
                     EAIFKHHLTSKEAVFDTLNWRTPNGCDKCRPAVNYYLISSWPHEAKDDPQSRFINERA
                     HANIQKDGTYSVVPRMWGGLTTPAELRAIADAAEKYQVPTVKVTGGQRIDLLGVKKED
                     LPAIWADLNAAGMVSGHAYGKSIRTVKTCVGAEHCRFGTQLAMDMGVKLEKMLFDMYA
                     PHKVKLAVSGCPRNCAEAGIKDVGVIGVDSGYELYVGGNGGIKTEVAQYLCKVGSDEE
                     VMEYAGAFLQLYREEGWYLERTVHWLDRVGLDYVKAKVVEDGENRRRLHARLLDALKD
                     ARDPWQTSREEEAVKQFIPIKVEA"
BASE COUNT          299 a          517 c          569 g          228 t
ORIGIN      
        1 cgcaccgcgg catcgcctac ggcctggtgg cgccgctgtt cgagcagggc aaggtgtgcg
       61 ccaaccacct cgccaacttc ggcatcggcc gctacgaggg ttcggtcacc tcgaccaagc
      121 tcaaggtcac cggcatcgac gtgttctcgg cgggcgactt cagcggcggc ccgggcaccg
      181 aggacatcgt gctgcacgac cgccagggcg gggtgtacaa gcgcgtcgtg ctcaaggacg
      241 accgcatcgt gggctcggtg ctgtacggcg acaccgccga cggctcgtgg tacttccagc
      301 tcatgaagga ccgccagagc gtcgccgaga tccgcgacca cctgatgttc gggcagaaca
      361 agctgggcga cgccggccac aagggcgaga accgcgccgc cagcatgccc gacacggccg
      421 aggtgtgcgg ctgcaacggg gtgtgcaagg gcaccatcgt caaggcgatc aaggagaagg
      481 gcctgttcac cctcgacgag gtcaagaagc acaccaaggc ggcgtcgtcc tgcggctcct
      541 gcaccggcct ggtcgagcag atcctcgcct ccaccgtcgg cggcgcctac caggcggtga
      601 gcgccaacga caagccggtg tgcgcgtgta ccgagcattc gcacaaggtg gtgcgcgagg
      661 cgatcttcaa gcaccacctg acgagcaagg aggcggtgtt cgacacgctg aactggagga
      721 cgcccaacgg ctgcgacaag tgccggccgg cggtcaacta ctacctgatc tcgtcgtggc
      781 cgcacgaggc caaggacgat ccgcagtcgc gcttcatcaa cgagcgtgcc cacgccaaca
      841 tccagaagga cggcacctat tcggtggtgc cgcggatgtg gggcgggctg accacgcctg
      901 ccgagctgcg cgccattgcg gatgccgcgg agaaatacca ggtgcccacg gtcaaggtca
      961 ccggcggcca gcgcatcgac ctgctcggag tgaagaagga ggatctgccg gcgatctggg
     1021 ccgacctcaa cgcggccggc atggtgtcgg gccacgccta cggcaagtcg atccgcaccg
     1081 tgaagacctg cgtcggcgcc gagcactgcc gcttcggcac gcagctggcg atggacatgg
     1141 gggtcaagct cgagaagatg ctgttcgaca tgtacgcgcc gcacaaggtg aagctcgcgg
     1201 tgtcgggctg cccgcgcaac tgcgccgagg cggggatcaa ggacgtcggc gtgatcgggg
     1261 tcgattccgg ctacgagctg tatgtgggcg gcaacggcgg catcaagacc gaggtcgcgc
     1321 agtatctgtg caaggtgggc agcgacgagg aggtcatgga gtatgccggc gccttcctgc
     1381 agctctaccg cgaggagggc tggtacctcg agcgcacggt gcattggctc gaccgcgtcg
     1441 gcctcgacta cgtgaaggcc aaggtggtcg aggacggcga gaaccgccgc cgcctgcacg
     1501 cgcgcctgct cgacgcgctc aaggacgcgc gcgatccgtg gcagaccagc cgcgaggagg
     1561 aggcggtgaa gcagttcatc ccgatcaagg tcgaggcctg agtttccggg cgc
//
LOCUS       DAOQNN010000628         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_71690_length_1611_cov_10.903599, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000628 DAOQNN010000000
VERSION     DAOQNN010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71690_length_1611_cov_10.903599"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..969)
                     /locus_tag="PKV97_18055"
     CDS             complement(4..969)
                     /locus_tag="PKV97_18055"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNB07845.1"
                     /translation="MKTTFLDFETAISELEEKIDQLRFVQDDPAVDISDEIKRLEAKS
                     QSLTKDLYAKLSPWQIAQVARHPQRPYTLDYASLMFTDFHELHGDRNYADDKAIVGGL
                     ARFNGQACVVIGHQKGRDTKEKIARNFGMPRPEGYRKAMRLMKLAEKFALPVFTFVDT
                     PGAYPGIGAEERGQSEAIGHSIYLMSELKTPIISTIIGEGGSGGALAIAVADRVLMLQ
                     YSTYSVISPEGCASILWKSAEKAADAAETMGITAARLKSLELVDKVVDEPIGGAHRDH
                     RAMAATLKRALADALRELEALSPSELVEQRYQKLMSYGRYKEKAA"
     gene            complement(1138..>1611)
                     /locus_tag="PKV97_18060"
     CDS             complement(1138..>1611)
                     /locus_tag="PKV97_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HNB07846.1"
                     /translation="FATTKFRDSSLGIVFVFALTGFMGFTLGPIISSYLALPNGGSIV
                     MQAMAGTAAIFLGLSAYAVTTKKDFSFMGGFLMVGILVAFLAGLGAIFFEIPALSLTV
                     SAAFVMLMSGLILYETSNIIHGGETNYIMATVSLFVSIFNLFTSLLHLLGFANND"
BASE COUNT          265 a          509 c          563 g          274 t
ORIGIN      
        1 gcctcaggcc gccttctcct tgtagcggcc gtagctcatc agcttctggt agcgctgctc
       61 gaccagctcg gagggcgaca gcgcctcgag ctcgcgcagc gcgtcggcga gcgcgcgctt
      121 gagcgtggcg gccatcgcgc ggtggtcgcg gtgggcgccg ccgatcggct cgtcgaccac
      181 cttgtcgacc agctcgagcg acttcaggcg cgccgcggtg atgcccatgg tctcggccgc
      241 gtcggcggcc ttctccgcgc tcttccacag gatcgaggcg caaccttcgg gcgagatcac
      301 cgagtaggtc gagtattgca gcatcagcac gcggtcggcg accgcgatcg cgagcgcgcc
      361 gcccgagccg ccttcgccga tgatggtgct gatgatcggg gtcttgagct cggacatcag
      421 gtagatgctg tgcccgatcg cctcggactg gccgcgctcc tcggcgccga tgccgggata
      481 ggcccccggc gtgtcgacga aggtgaacac cggcagcgcg aacttctcgg ccagcttcat
      541 caggcgcatc gccttgcggt agccctcggg gcgcggcatg ccgaagttgc gcgcgatctt
      601 ttccttggtg tcgcgaccct tctgatgacc gatcaccacg caggcctggc cgttgaagcg
      661 cgccagcccg cctacgatgg ccttgtcgtc ggcgtagttg cggtcgccgt gcagctcgtg
      721 gaagtcggtg aacatcaggc tggcgtagtc cagcgtgtag gggcgctgcg ggtggcgcgc
      781 gacctgggcg atctgccagg ggctgagctt ggcgtagagg tccttggtca gcgactggct
      841 cttggcctcg agccgcttga tctcgtcgga gatgtcgacc gccggatcgt cctggacgaa
      901 gcgcagctgg tcgatctttt cctcgagttc ggagatcgcc gtttcgaaat caagaaaggt
      961 ggtcttcatc ccgggctgtc ccagtcaaaa atgtgcgcca ttgtaacgcc agccgcccgg
     1021 ccgcgcgcgg cgcgcgtgct gcagcgcaaa acccgtgctc gcgcgcaaaa gaatggggcg
     1081 gcgcctcgcg gccaccgccc cattccggaa ggcgcccgcc gtggcgggcg ctccgggtca
     1141 gtcgttgttg gcgaagccca gcaggtgcag caggctggtg aagaggttga agatcgacac
     1201 gaacagcgac accgtggcca tgatgtagtt ggtctcgccg ccgtggatga tgttgctggt
     1261 ctcgtacagg atgaggcccg acatcagcat cacgaaggcc gccgagacgg tcagcgacag
     1321 cgccgggatc tcgaagaaga tcgcgcccag gccggcaagg aaggccacca ggatgccgac
     1381 catcaggaag ccgcccatga aggagaagtc cttcttggtg gtcaccgcgt aggccgacag
     1441 gccgaggaag atcgccgcgg tgccggccat cgcctgcatc acgatcgagc cgccattggg
     1501 cagggcgagg taggacgaga tgatcgggcc gagggtgaag cccatgaagc cggtcagcgc
     1561 gaacacgaac acgatgccca ggctgctgtc gcggaacttg gtggtggcga a
//
LOCUS       DAOQNN010000629         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_72020_length_1607_cov_7.795747, whole genome shotgun sequence.
ACCESSION   DAOQNN010000629 DAOQNN010000000
VERSION     DAOQNN010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72020_length_1607_cov_7.795747"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1607)
                     /locus_tag="PKV97_18065"
     CDS             complement(<1..>1607)
                     /locus_tag="PKV97_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB07847.1"
                     /translation="HVDLLDHVSFQLDAGERVALIGRNGSGKSSLLRALAGQAALDDG
                     TVWRQAEMVTAYVPQEPDFELGRDVFETIADGLGAAARLLVDYHAAMLEVAEHASPEA
                     MARLDGLQHAVEAAGGWRLNQRVEQVVARLGLDPAAKVSSLSGGGVKRVALARALAAE
                     PDLLLLDEPTNHLDLDGILWLEGLIRDFRGAVIVITHDRVFLDNVATRIIELDRGRLA
                     SYPGRFSDYQRRKAEELEAEEKAAARFDKFLAQEEVWIRKGVEARRTRNEGRVRRLEA
                     LRNARAARRDRLGDVSLAVDTGDKSGQMVAELTDVSKRYGDKVVVRDFSTRILRGDRI
                     GFIGPNGAGKTTLLKLILGEVEPDAGTVRRGTRQTVAYFDQLRAQLDPELPLTEVISP
                     GSDFVEIGGEKKHVIGYLGDFLFAPQRARSPVKSLSGGERNRLLLARLFARPANVLVL
                     DEPTNDLDIETLDLLEELLAGYDGTLFLVSHDRAFLDNVVTQVIAAEGDGRWSEYAGG
                     YGDWQRVQARRAEEAAAREATARESAR"
BASE COUNT          218 a          553 c          593 g          243 t
ORIGIN      
        1 cctggcgctc tcgcgcgccg tcgcctcgcg cgcggcagcc tcctcggcac gccgtgcctg
       61 aacgcgctgc cagtcgccgt agccgccggc gtattcgctc caacgcccgt cgccctcggc
      121 ggcgatcacc tgggtgacca cgttgtcgag gaaggcgcgg tcgtggctga cgaggaagag
      181 cgtgccgtcg tagccggcaa gcagctcctc cagcaggtcg agggtctcga tgtcgaggtc
      241 gttggtgggc tcgtcgagca ccagcacgtt ggccgggcgg gcgaacagac gcgccagcag
      301 caggcggttg cgctcgccgc cggagagcga cttgaccggt gagcgcgcgc gctgcggcgc
      361 gaacaggaag tcgccgaggt agccgatgac gtgcttcttc tcgccgccga tctcgacgaa
      421 atccgagccc gggctgatga cctcggtgag cggcagctcg gggtcgagct gggcgcgcag
      481 ctggtcgaag taggccaccg tctgccgggt gccgcggcgc accgtgcccg cgtcgggctc
      541 gacctcgccg aggatgagct tcaacagcgt ggtcttgccg gcgccgttgg ggccgatgaa
      601 gccgatgcga tcgccgcgca ggatgcgggt ggagaaatcg cgcaccacca ccttgtcgcc
      661 gtagcgcttg gagacgtcgg tgagctccgc gaccatctgg ccgctcttgt cgccggtgtc
      721 gaccgccagg ctgacgtcgc cgaggcggtc gcgacgcgcg gcgcgcgcgt tgcgcagggc
      781 ctcgagccga cgcacgcggc cttcgttgcg ggtgcgccgg gcctccacgc ccttgcggat
      841 ccacacctcc tcctgggcga ggaacttgtc gaagcgcgcg gcggccttct cctcggcttc
      901 gagctcttcc gccttgcggc gctggtagtc ggagaagcgc cccgggtagc tcgccagccg
      961 gccgcggtcg agctcgatga tgcgggtggc gacgttgtcg aggaagacgc ggtcgtgggt
     1021 gatgacgatg accgcgccgc gaaagtctcg gatcagccct tcgagccaca ggatgccgtc
     1081 gaggtcgagg tggttggtgg gttcgtcgag cagcagcagg tcgggctcgg cggccagcgc
     1141 gcgcgccagc gccacgcgct tgacgccgcc gccggagagg ctggaaacct ttgccgccgg
     1201 gtcgagtccg agtcgggcga ccacctgctc gacgcgctgg ttgagccgcc agccgcccgc
     1261 ggcctcgacc gcatgctgca ggccgtccag ccgggccatg gcctcggggc tggcgtgctc
     1321 ggcgacctcc agcatggcag catggtagtc gacgagcagg cgggcggcgg cgcccaggcc
     1381 gtcggcgatg gtctcgaaga cgtcgcgccc gagctcgaaa tccggctcct gcggcacgta
     1441 tgccgtgacc atttcagcct gccgccacac tgtgccatca tcgagcgccg cctggccggc
     1501 gagcgcgcgc agaaggctgg acttgcccga gccgttgcgc ccgatgagcg ccacgcgctc
     1561 cccggcgtcg agctggaagc tcacgtgatc gagcaggtcg acgtgac
//
LOCUS       DAOQNN010000630         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_72190_length_1605_cov_10.575484, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000630 DAOQNN010000000
VERSION     DAOQNN010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72190_length_1605_cov_10.575484"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKV97_18070"
     CDS             <1..462
                     /locus_tag="PKV97_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNB07848.1"
                     /translation="VEMVTGTDKVTLVEIHDPSMVGTVGFDYGLMEIFCKHEISYILK
                     ATNANSIAHLVWDSSVTTELVEELEARYQVVTVKKSAIVCAIGSNIGIPGVLARAAQA
                     MAEAGVNVNCISQTLRQVNMQFVIERGDYKKAVIALNQALCVAPGTVVPKA"
     gene            complement(496..633)
                     /locus_tag="PKV97_18075"
     CDS             complement(496..633)
                     /locus_tag="PKV97_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07849.1"
                     /translation="MMYTSGYTGNAIQQLDATLTPVHLITKPYAIDEMTRAVRALLDA
                     D"
     gene            719..1597
                     /locus_tag="PKV97_18080"
     CDS             719..1597
                     /locus_tag="PKV97_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07850.1"
                     /translation="MDWHTQLITLYLAVCKYWQEVGWALAQRHAPYADLGFTDEEVVT
                     IYLFAVALEEKRQIKSIHQHARRYWRDWFPRLPGYGAYVQRLNHLADCFPSLLERFCE
                     TGAAASVAGLVDSMPVILARQGRRFNAKVAPELASSGYCPTKHLYYYGVKIHLVGDRR
                     PDTLPSPRFIGLAPAGLNDGPALEQVAPALPYKDLYADKAYEYLKRVSGLPFTVLTPV
                     KKQKGQEHLDAADLWLSRAVSQVRQPVESLFNWIEEKTGIEIASKVRSSQGLLVHVFG
                     RLAAAMFVRNVLPLST"
BASE COUNT          298 a          522 c          489 g          296 t
ORIGIN      
        1 gtcgagatgg tcaccggcac cgacaaggtg acgctggtcg agatccacga cccctcgatg
       61 gtcggcaccg tcggcttcga ttacggcctg atggagatct tctgcaagca cgagatcagc
      121 tacatcctga aggccaccaa cgccaactcg atcgcccacc tggtctggga cagttcggtg
      181 accacggaac tggtcgaaga gctcgaggcg cgctaccagg tcgtcacggt gaagaagagc
      241 gccatcgtct gcgcgatcgg ctccaacatc ggtatcccgg gcgttttggc gcgcgccgcg
      301 caggcgatgg cggaagccgg cgtcaatgtc aattgcatat cgcagacctt gcgccaggtg
      361 aacatgcagt tcgtgatcga gcgcggcgac tacaagaagg cggtcatcgc cctcaaccag
      421 gcgctctgcg ttgctccggg aacggtggtg ccgaaggcct gacgttcggc gggcgaccga
      481 tcgcgcgcgc ccgattcaat cggcgtcgag cagcgcgcgc actgcgcgcg tcatctcgtc
      541 gatcgcgtac ggcttggtga tcaggtgcac cggcgtcagc gtggcgtcga gttgctggat
      601 cgcgttgccg gtatagcccg acgtgtacat caccttgaat gcgaattcag agctaacggt
      661 gccagcccaa agttctatcc tgtgaggctg ttccccaaca acctgaccga gcacgacgat
      721 ggactggcac acgcagttga taacactcta ccttgcggtt tgtaaatatt ggcaagaggt
      781 gggctgggcg ctggcccagc gccacgcgcc ttatgccgac ctgggcttca ccgacgagga
      841 ggtcgtcacg atctacctgt ttgccgtcgc tctggaagag aagcgccaga tcaagagcat
      901 ccaccagcat gcccggcgct attggcgcga ctggtttccc cggctgcccg gctacggggc
      961 ctatgtgcag cgcctgaacc acctcgcgga ctgctttcct tccctgctgg agcggttctg
     1021 cgagacgggc gcggcggcat ccgttgcggg gctggtcgat tccatgccgg tgatcctcgc
     1081 ccggcaagga cgccgcttca acgccaaagt agcccccgaa ctggccagca gtggctactg
     1141 cccgaccaag cacctctact actacggcgt gaaaatccac ttggtcggcg accgacggcc
     1201 cgacacgctg ccaagcccgc gcttcatcgg tcttgcgccg gcgggcctga acgacggccc
     1261 ggcgctggag caggtcgccc ccgcgctgcc ctacaaggac ctctacgccg acaaggcgta
     1321 tgaatacctc aaacgcgtgt ccgggctgcc gttcacggtg ctgacgcccg tcaagaagca
     1381 gaagggccag gaacaccttg atgccgctga cctctggctt tcccgggccg tctcgcaggt
     1441 gcgccagcct gtcgagtccc tcttcaactg gatcgaagag aaaaccggca tcgagatcgc
     1501 cagcaaagtg cgctcctccc aagggcttct cgtccatgtc ttcgggcggc tcgccgccgc
     1561 catgttcgtg cgaaacgtct tgccgctaag cacttaattt gcatt
//
LOCUS       DAOQNN010000631         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_72192_length_1605_cov_7.363226, whole genome shotgun sequence.
ACCESSION   DAOQNN010000631 DAOQNN010000000
VERSION     DAOQNN010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72192_length_1605_cov_7.363226"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..789
                     /locus_tag="PKV97_18085"
     CDS             205..789
                     /locus_tag="PKV97_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HNB07851.1"
                     /translation="MLGNSERKRVHTRRIVCEAFRRRDGLWEIEATVIDEKGEDMPFR
                     SRPMVRPGQSLHDITIAVLIDDDAVIHDVAARMQTVPWAACPESQAAYRRLIGLQIGA
                     GFMREVRERVGGVAGCTHLTDLLVQVGNTYTQAIWPTLIARQFAAEPDPRKWTDRRAV
                     GFVGGCAAWRQDGDTLRQEYPELAAEFVAKRDET"
     gene            complement(827..1279)
                     /locus_tag="PKV97_18090"
     CDS             complement(827..1279)
                     /locus_tag="PKV97_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07852.1"
                     /translation="MSIQGLGSSASTASWVGAQLSREQARRSAEQLEAKAAALSAEAA
                     SARDEADTAQRRADELQTQAGQARTRAEIGKQAVASVRGMDRVAQTLSAAVGRLADAA
                     EAGPSQGLGLQESGRIPAPTASSDLYTPAAGVRPALSVQPGQVVDLKV"
     gene            complement(1321..>1605)
                     /locus_tag="PKV97_18095"
     CDS             complement(1321..>1605)
                     /locus_tag="PKV97_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07853.1"
                     /translation="QVAQPSAVVTLSAAARGEGVAQPPAQGGSVEARAVEQARAAAAN
                     GDVRSNYSAQRALASYEAMSQVGANNAAARPSQPEQPTAPGQREPGVLRT"
BASE COUNT          237 a          572 c          558 g          238 t
ORIGIN      
        1 tggtattcgc catcctccgc gaacagggcg cagaactgcg cctcgtcgcg ttcctcccag
       61 gccttcttgt acccttgcat gaattgttcg atatccatct tttagcctcc tcgctctctt
      121 gttatcgtcc acctcccagc cacggcagta taggcgccac ggcgcgcggg ccacgaaggc
      181 ggaacggatc agagggcgga cgacatgctg ggaaacagcg aacggaaacg ggtgcacacg
      241 cgccgcatcg tgtgcgaggc ctttcggcgc cgcgacgggc tgtgggaaat cgaggcgacg
      301 gtgatcgacg agaagggcga ggacatgccc ttccgctctc gtccgatggt gcgcccgggg
      361 caatccctcc acgacatcac gatcgccgtg ctgatcgatg acgacgccgt gatccacgac
      421 gtcgccgcgc ggatgcagac cgtgccctgg gcggcctgtc cggaaagcca ggccgcctac
      481 cgccgcctga tcgggctgca gatcggcgcg ggcttcatgc gcgaggtccg cgagcgcgtg
      541 ggcggggtgg cgggctgcac ccacctcacc gacctgctcg tgcaggtcgg caacacctac
      601 acccaggcga tctggccgac gctgatcgcc cgccagttcg ccgccgagcc tgatccgcgc
      661 aaatggaccg accgccgcgc ggtcggcttc gtcggcggct gcgcggcgtg gcggcaagac
      721 ggggacacac tcaggcagga atatccggag ctggcggcgg agttcgtcgc gaagcgggac
      781 gaaacctgag gccagaaccc ggcaacgccc acccgccggg cgcaggtcac accttcagat
      841 cgacgacctg cccgggctgg acgctcagcg caggccggac accggccgcg ggggtgtaca
      901 ggtcagagga tgctgtgggc gcggggatcc ggccggattc ctgcagccca agcccctggg
      961 aagggcccgc ctcggccgca tcggcgagcc ggccgaccgc cgccgataag gtctgcgcca
     1021 cacggtccat gccacgcacc gaggctaccg cttgcttgcc gatttccgcg cgggtgcgtg
     1081 cctgccccgc ctgggtctga agttcgtcgg cgcgacgttg ggcagtgtcc gcctcgtcac
     1141 gcgcgctcgc agcctccgcg gagagcgcgg ccgccttggc ctcgagctgt tcggcacttc
     1201 gccgtgcctg ctcgcgcgag agctgcgcgc cgacccagga ggccgtggag gcggaggatc
     1261 cgagaccttg tatggacatg atggtcagcc ctcagcgggc ttgagcaccg ctgcggggac
     1321 tcaggtacgc aacacgcccg gctcgcgctg gcccggcgcc gtcggttgct caggctggct
     1381 cgggcgcgcg gcagcattgt tggcgcccac ctgcgacatc gcctcgtagg acgcgagcgc
     1441 acgttgcgcg gagtagttgc tgcgcacgtc gccattggcc gcggcggcgc gggcctgctc
     1501 gaccgcacgt gcctcgaccg agccgccctg cgcaggcggc tgcgccacgc cctcgccacg
     1561 cgcggccgcg ctcagcgtga ccaccgcgct cggctgcgcg acctg
//
LOCUS       DAOQNN010000632         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_72276_length_1604_cov_8.189800, whole genome shotgun sequence.
ACCESSION   DAOQNN010000632 DAOQNN010000000
VERSION     DAOQNN010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72276_length_1604_cov_8.189800"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PKV97_18100"
     CDS             <1..1145
                     /locus_tag="PKV97_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07854.1"
                     /translation="ELRARAHALAHARSVEVLATARAALIGYAISYAETHPGEGYGFL
                     PCPDAGNTGSTPIGACGARGTAAIGRLPWRTLGLPELRDGWGECLWYAVAGSVKHNPK
                     PAALNWDSPGQFGLLDPAGRSLQAASGDGMAVAIVFSPGPPRSSQIRPPTRGTSCTGS
                     DSAAADLSHYLDPGHARAGSGVIEITLHPATLDDEAPNDLATWIGIDDVFDALRRRRD
                     HASHLDALLARSANALAGRLAASRTEWLARHARPLGTLAVGMLPTAETLGIAAAQRPL
                     IDDWHEQIRFAACPDGSACIGVTRPAAPGERCRAVLAFGGERIRSGPERQLRTTAAER
                     ADPAAWFEGRNVAALRLGNPEFEGAAHFRVTAPDRPASEDVVRCLP"
     gene            1163..>1604
                     /locus_tag="PKV97_18105"
     CDS             1163..>1604
                     /locus_tag="PKV97_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNB07855.1"
                     /translation="MHKPAHSSAPARGFTLVELAMVLVILALLGGSLLVPVASRLEAR
                     DRNATLERLHDIQLALTGFAIIHGRLPCPSTETDPASPGYGLEDGPPCNLAREGVLPW
                     RSLAMPATDAWGRDRHSAADDWTGYWRYRVDPAFSVAPIGAATTP"
BASE COUNT          214 a          665 c          529 g          196 t
ORIGIN      
        1 gcgaactgcg cgcccgcgcc cacgcgctcg cccacgcgcg cagcgtcgag gtgctcgcca
       61 ccgcacgcgc cgcgctgatc ggctacgcga tcagctacgc cgagacccac cccggagagg
      121 gctacggttt cctgccctgt ccggatgccg gcaacaccgg ctccaccccg atcggtgcct
      181 gcggtgcgcg cggcaccgcc gcgatcgggc gcctgccctg gcgcaccctc ggcctgcccg
      241 agctgcgcga cggctggggc gaatgcctgt ggtacgcggt cgccggcagc gtcaagcaca
      301 accccaagcc ggcggcgttg aactgggaca gcccgggcca gttcggcctg ctcgatcccg
      361 ccgggcgcag ccttcaggca gcgtccggcg acggcatggc ggtggcgatc gtgttcagcc
      421 ccgggccgcc acgcagcagc cagatccgtc cgcccacccg gggcacgagc tgcacaggca
      481 gcgactcggc cgcagccgac ctttcgcact acctcgaccc cggccatgcg cgcgcaggca
      541 gcggggtgat cgagatcacc ctccacccgg ccaccctcga cgacgaggcg cccaacgacc
      601 tcgccacctg gatcggaatc gacgacgtct tcgacgcact gcgtcggcgc cgcgaccacg
      661 ccagccatct cgatgcgctc ctcgcccgca gcgcgaacgc gcttgccggc cggcttgcgg
      721 cgagccgcac cgaatggctc gcacgccacg cccgcccgct cggcacgctc gcggtgggta
      781 tgctgcccac ggcggagacc ctgggcatcg ccgccgcaca acgtccgctc atcgacgact
      841 ggcacgagca gatccgcttc gccgcctgcc ccgacggcag cgcctgcatc ggcgtgacgc
      901 ggcccgcggc acccggcgag cgctgccggg cggtgctggc attcgggggc gagcgcatcc
      961 gcagcggccc cgagcgccaa ctgcgaacca ccgctgccga gcgcgccgat ccggcggcct
     1021 ggttcgaggg ccggaacgtc gccgcgctac gcctgggcaa tcccgagttc gagggtgccg
     1081 cgcacttccg cgtcaccgcg cccgatcgcc ccgccagcga ggacgttgtc cgatgcctgc
     1141 cctgaccccg cctcccgctc gcatgcacaa gcccgcgcat tcgagcgctc ccgcgcgcgg
     1201 cttcacgctg gtcgagctcg ccatggtgct ggtgatcctc gccctgcttg gcggcagcct
     1261 gctggtcccg gtggcgagcc ggctggaggc gcgcgaccgc aacgccacgc tcgagcgtct
     1321 gcacgacatc cagctcgcgc ttaccggctt cgccatcatc cacggccgcc tgccctgccc
     1381 gagcaccgaa accgatcccg ccagccctgg ctacgggctc gaggacggcc cgccctgcaa
     1441 cctcgcgcgc gagggcgtgc tgccctggcg cagcctggcg atgccggcga ccgacgcctg
     1501 gggccgcgac cgccactccg ccgccgacga ctggacgggg tactggcgct accgcgtcga
     1561 ccccgccttc agcgtggcgc cgatcggcgc cgccaccaca cccg
//
LOCUS       DAOQNN010000633         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_72344_length_1603_cov_13.221576, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000633 DAOQNN010000000
VERSION     DAOQNN010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72344_length_1603_cov_13.221576"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="PKV97_18110"
     CDS             complement(<1..1191)
                     /locus_tag="PKV97_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HNB07856.1"
                     /translation="MDLPQKQGLYDPANEHDACGVGFIAHIKGEKRHDIILQGLEILK
                     NLDHRGAVGADPLQGDGAGILIQIPDQLYREEMAAQGVTLPLPGEYGVGMVFMPKEAA
                     SRLACEQEIERAVVAEGQVVLGWRDVPVNRDMPMSPTVKAMEPVIRQIFIGRGPDVMV
                     TEALERKLYVVRRRSANAIGALKLKHAKEFYMVSCSARTVNYKGLLLADQVGEYYPDL
                     LDKRAVSALSLVHQRFSTNTFPKWNLAHPFRYIAHNGEINTLRGNYNWMRAREKGVHS
                     PLLGDDLQKIWPLIYPGQSDSASFDNALELLVMSGYSLAHAMMMMIPEAWESHTQMDE
                     KRRAFYEYHAAMMEPWDGPAAVAFTDGRQIGATLDRNGLRPARYLITDDDLVVMASES
                     GVLPI"
BASE COUNT          277 a          519 c          532 g          275 t
ORIGIN      
        1 gatgggcagc acgccggact cggaggccat caccacgagg tcgtcgtcgg tgatcaggta
       61 gcgcgccgga cgcaggccgt tgcggtcgag ggtggcaccg atctggcggc cgtcggtaaa
      121 ggccaccgcc gcggggccgt cccaaggctc catcatcgcc gcgtggtact cgtagaaggc
      181 gcggcgcttc tcgtccatct gcgtgtgcga ctcccaggcc tcggggatca tcatcatcat
      241 cgcgtgggcg agcgagtagc cgctcatcac cagcagctcg agcgcgttgt cgaaggaggc
      301 cgagtcggac tggccggggt agatcagcgg ccagatcttc tgcaggtcgt cgccgagcag
      361 cggcgaatgc acgcccttct cgcgcgcgcg catccagttg taattgccgc gcagggtgtt
      421 gatctcgccg ttgtgggcga tgtagcggaa cgggtgggcg aggttccact tcgggaaggt
      481 gttggtcgag aagcgctggt gcaccagcga cagcgccgac acggcacgct tgtcgagcag
      541 gtcggggtag tactcgccga cctggtcggc gagcagcagg cccttgtagt tcacggtgcg
      601 cgccgagcac gacaccatgt agaactcctt ggcgtgcttg agcttgagcg cgccgatggc
      661 gttggcggag cggcggcgca cgacgtagag cttgcgctcg agcgcctcgg tgaccatgac
      721 gtcgggaccg cggccgatga agatctggcg gatcaccggc tccatggcct tgaccgtggg
      781 cgacatcggc atgtcgcggt tgaccggcac gtcgcgccag ccgagcacga cctggccttc
      841 ggccacgacc gcacgctcga tctcctgctc gcaagcgagg cgcgaggcgg cttccttggg
      901 catgaacacc atgccgacgc cgtactcgcc gggcaacggc agggtcacgc cctgcgccgc
      961 catctcctcg cggtagagct ggtcggggat ctggatcagg atgcccgcgc cgtcgccctg
     1021 cagcgggtcg gcccccaccg cgccgcgatg gtcgaggttc ttcaggatct cgaggccctg
     1081 cagaatgatg tcatgacgtt tctcgccctt gatgtgggcg atgaaaccca cgccacaggc
     1141 atcgtgttca ttggcggggt cgtacagacc ctgcttttgg ggcagatcca tcttcgattc
     1201 ctcaaacgac actcaagatc acgtgctcgg ggtcggacga aatcggccga aaggccgtcg
     1261 ccgacaaacc gaacgcgcac aaaaaagccg ggcgcgcgcg caccggcttt gtagtctgca
     1321 ccaatctggt gcagaaacag cgattccacg gggtcgaagg gcgacctttt caggcctcat
     1381 gcaccatttt ggacactacc gtcctgttcg ctgcggtcga aaaactatac cccccaaagc
     1441 tcgccggatt caacagtttt tttgcactgc ggcacgcgac gaaaacaccc gccgggcggc
     1501 ctctccgaga ccccgcagtc gccccccgca gccgaatggc accgcgacgc tcggcttgcc
     1561 cgtgggagcg acgccagtcg cgattcggcg aacccgatgt gcg
//
LOCUS       DAOQNN010000634         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_72409_length_1602_cov_9.643180, whole genome shotgun sequence.
ACCESSION   DAOQNN010000634 DAOQNN010000000
VERSION     DAOQNN010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72409_length_1602_cov_9.643180"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PKV97_18115"
     CDS             <1..545
                     /locus_tag="PKV97_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNB07857.1"
                     /translation="SAALYYLIHSTLAAGALGLVADLVAERRGAQRDRFAAAPRIAQG
                     GLVGGLFFVAAIAMTGMPPLSGFIGKLLVLDGVRASAHAAWFWALILTTSLIAIVGFA
                     RAGSFVFWKAHALPAAPASAYRPDGALSFVAVGGLLACLVLLTVFAGPVHAWLELVAA
                     QLYAPAGYIDAVLGTAQGVR"
     gene            545..1072
                     /locus_tag="PKV97_18120"
     CDS             545..1072
                     /locus_tag="PKV97_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNB07858.1"
                     /translation="MGAKHDKLDPRSFTERWLPHPLMSAVLVLLWMLLLNSFSFGGLV
                     VGVVLAVVITRVTSNFWPERPPVKSWTKAFAYLGVVAWDVVVANLQVTRLILFRPVGS
                     LATRWVVVPLELRSPEAITVLAGTITMTPGTVSCDLSADGRSLLVHCLDAPDAEEAVR
                     QMKERYEDRLQEIFP"
     gene            1069..1338
                     /locus_tag="PKV97_18125"
     CDS             1069..1338
                     /locus_tag="PKV97_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HNB07859.1"
                     /translation="MIEYALQFGFATASLALLLNLWRLLRGPALMDRVLAVDTMVINI
                     IALIVLAGIQQRATHFFEAALLFAMFGFISTVAYCRFILRGDVIE"
     gene            1352..>1602
                     /gene="mnhG"
                     /locus_tag="PKV97_18130"
     CDS             1352..>1602
                     /gene="mnhG"
                     /locus_tag="PKV97_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HNB07860.1"
                     /translation="MTFVLELLVSLLIVAGGSFLLIGSIGMVKLPDLLTRLHAPTKAT
                     TLGVGGALAASMVFFAGLEGTLSIHEVLITAFLFMTAPL"
BASE COUNT          183 a          609 c          520 g          290 t
ORIGIN      
        1 ccagcgccgc gctgtactac ctcatccact ccacgctcgc cgccggcgcg ctcggtctcg
       61 tcgccgatct cgtcgccgag cgccgcggcg cgcagcgcga ccgcttcgcc gcggcgccgc
      121 gcatcgccca gggcgggctg gtcggcggcc tgttcttcgt cgccgccatc gccatgaccg
      181 gcatgccgcc gctgtccggc ttcatcggca agctgctggt gctcgacggc gtgcgcgcct
      241 cggcgcacgc ggcctggttc tgggcgctga tcctgacgac ctcgctgatc gccatcgtcg
      301 gcttcgcgcg cgccggcagc ttcgtgttct ggaaggcgca cgcgctgccc gccgcgcccg
      361 cgtccgccta tcgcccggac ggcgcgctgt ccttcgtcgc cgtgggcggg ctgctggcct
      421 gcctggtgct gctcaccgtg ttcgccggcc cggtgcatgc ctggctggag ctcgtcgcgg
      481 cgcagctcta cgccccggcc ggctacatcg acgcggtgct cggcaccgca cagggggtgc
      541 gctgatgggc gcgaagcacg acaagctcga cccgcgcagc ttcaccgaac gctggctgcc
      601 gcatccgctc atgagcgcgg tgctggtgct gctgtggatg ctgctgctca acagtttcag
      661 cttcggcggg ctggtggtgg gcgtggtgct cgcggtggtg atcacgcggg tgacgagcaa
      721 cttctggccc gagcgcccgc cggtcaagtc ctggaccaag gccttcgcct acctcggcgt
      781 ggtggcctgg gacgtggtgg tcgccaacct gcaggtcacc cgcctgatcc tgttccggcc
      841 ggtgggcagc ctggccacgc gctgggtggt ggtgccgctc gagctgcgct cgcccgaggc
      901 catcaccgtg ctcgccggca ccatcacgat gacgcccgga acggtgtcct gcgacctctc
      961 cgccgacggc cgcagcctgc tcgtgcactg cctcgacgcg cccgacgccg aggaggccgt
     1021 gcgccagatg aaggaacgct acgaggaccg cctccaggag atcttcccat gatcgaatac
     1081 gccctgcagt tcggcttcgc caccgcctcg ctcgcgctgc tgctcaacct gtggcggctg
     1141 ctgcgcggcc ccgcgctcat ggaccgcgtg ctcgcggtcg acaccatggt catcaacatc
     1201 atcgcgctga tcgtgctcgc cggcatccag cagcgcgcca cccacttctt cgaggccgcg
     1261 ctgctgttcg cgatgttcgg cttcatctcc accgtcgcct actgccgctt catcctgcgc
     1321 ggcgacgtca tcgagtgagc ggagacccct catgaccttc gtgctcgaac tcctcgtctc
     1381 gctgctgatc gtcgccggcg gcagcttcct gctcatcggc tccatcggca tggtcaagct
     1441 gcccgacctg ctcacccgcc tgcacgcgcc caccaaggcg accacgctcg gcgtgggcgg
     1501 cgcgctcgct gcgtccatgg tgttcttcgc cgggctggag ggcacgctgt ccatccacga
     1561 ggtgctgatc accgccttcc tgttcatgac cgcaccgctc ag
//
LOCUS       DAOQNN010000635         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_73187_length_1593_cov_7.739922, whole genome shotgun sequence.
ACCESSION   DAOQNN010000635 DAOQNN010000000
VERSION     DAOQNN010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73187_length_1593_cov_7.739922"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PKV97_18135"
     CDS             <1..574
                     /locus_tag="PKV97_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HNB07861.1"
                     /translation="KQVLLIMDSLTRYAMAQREIALAIGEPPVTRGYPPSVFARLPAL
                     VERAGNGPEGGGSITAFYTVLAEGDDQQDPIADAARAILDGHFVLSRNLADQGHYPAI
                     DIEQSISRAMHNLVGDDHFNVVRQFKQFFSRYQRSRDLIAVGAYQPGGDPLLDMAVKL
                     YPRLEAFLQQGIQEHETYEGALQKLAQVFG"
     gene            819..1259
                     /gene="fliJ"
                     /locus_tag="PKV97_18140"
     CDS             819..1259
                     /gene="fliJ"
                     /locus_tag="PKV97_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNB07862.1"
                     /translation="MPASFHLQPLLDLASTRTDEAARRLGELVAAERDVEQKLKMLED
                     YRTEYHERFVQAARDGLSADAWRNYGAFIAKLDDAIAAQRKVVEQSRARTVEGQQAWL
                     AQRTKLKAFDTLAQRHQHAVARSEARQEQKQSDEHAARRGGEDA"
     gene            1379..>1593
                     /locus_tag="PKV97_18145"
     CDS             1379..>1593
                     /locus_tag="PKV97_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07863.1"
                     /translation="MSAPFSPSSLGNVAQLRLGAQQARIGISALDGLGAQPDSFSRTL
                     ENRMNTPEPAPRKPESPPPAPRPPERTA"
BASE COUNT          275 a          623 c          494 g          201 t
ORIGIN      
        1 caagcaggtg ctgctgatca tggactccct cacccgctac gccatggccc agcgcgagat
       61 cgcactggcc atcggcgagc cgcccgtcac ccgcggctac ccgccgtcgg tgttcgcgcg
      121 gctgccggca ctcgtcgagc gcgccggcaa cggccccgag ggcggcggct cgatcaccgc
      181 cttctacacc gtgctcgccg agggcgacga ccagcaggac ccgatcgccg acgcggcgcg
      241 cgccatcctc gacggccact tcgtgctctc gcgcaatctc gccgaccagg gccactaccc
      301 ggcgatcgac atcgagcagt cgatctcgcg cgcgatgcac aacctcgtcg gcgacgacca
      361 cttcaacgtg gtgcgccagt tcaagcagtt cttctcgcgc taccagcgtt ctcgcgacct
      421 gatcgccgtc ggcgcctacc agcccggcgg cgatcccctg ctcgacatgg ccgtcaagct
      481 ctaccccagg ctcgaagcct tcctgcaaca aggcatccag gaacacgaga cctacgaggg
      541 tgccctgcag aagctggcgc aggtgttcgg gtgagaaagg cacgaggcgc aagcgctccc
      601 gctcgcccgt aggagcgaag ccagccgcga tcgggcggcg aagctgcgcc gccgcggggc
      661 cgaagcgccg cgatcgcgac tggcgtcgct cctgcacccg cgacatcgat cgcgagcatt
      721 tcgtcacggt cgaggacgag ccgaccgaaa tatgcccacc tcgggcatcc ggcgaacggc
      781 tagaatgctg tttgcaacgc ccccgtgaca cgacgccgat gcccgcctcc ttccacctcc
      841 agcccctgct cgacctcgcc agcacccgca ccgacgaggc tgcgcgtcgc ctgggcgaac
      901 tcgtcgccgc cgaacgcgac gtggaacaga agctgaagat gctcgaggac tatcgcacgg
      961 aatatcacga gcgcttcgtg caggccgcgc gcgacggcct gagcgcggac gcatggcgca
     1021 actatggcgc cttcatcgcc aagctcgacg acgccatcgc cgcgcagcgc aaggtggtcg
     1081 agcaatcgcg cgcgcgcacc gtcgagggcc agcaggcctg gctcgcccag cgcaccaagc
     1141 tcaaggcctt cgacaccctc gcccagcgcc accagcacgc ggtcgcgcgc agcgaggcgc
     1201 gccaggagca gaagcagtcg gacgagcacg ccgcccgacg cggtggcgag gacgcctgac
     1261 gcgggaagcc gcggcagccg cccgacccgg tgacacaccc atggcatgag gcttgctttt
     1321 gatgggctgt cggatcgtcc gcgatccatc gcatccagcc cacgagaggc ccgcaaccat
     1381 gtccgcaccg ttctccccct ccagtctcgg caacgtcgcg cagcttcgcc tcggcgcgca
     1441 gcaggcacgc atcggcatct ccgccctgga cggcctgggc gcgcagcccg acagcttctc
     1501 gcgcacgctc gagaaccgca tgaacacgcc cgagccagcg ccgcgcaagc ccgagtcgcc
     1561 ccccccggcc ccgcgaccgc ccgagcgcac ggc
//
LOCUS       DAOQNN010000636         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_73445_length_1590_cov_6.381759, whole genome shotgun sequence.
ACCESSION   DAOQNN010000636 DAOQNN010000000
VERSION     DAOQNN010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73445_length_1590_cov_6.381759"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PKV97_18150"
     CDS             complement(<1..721)
                     /locus_tag="PKV97_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNB07864.1"
                     /translation="MSIHVKLNHVTRYRYDRPVALSPQVVRLRPAPHCRTPIHAYALK
                     VEPAGHFINWQQDPQSNYLARLVFPDKTTELRIEVDLVAELSVINPFDFFLEPAAEKI
                     PFAYEEALRVELAPYLAKAPAAALGPKFIDYLESIPRAPRASVDFLVALNQRLQQDIR
                     YLVRLEPGVQTPEETLTLASGSCRDSAWLLVQLLRHLGLAARFVSGYLIQLVPDVKSL
                     DGPSGTEVDFTDLHAWCEVYLP"
     gene            1131..>1590
                     /locus_tag="PKV97_18155"
     CDS             1131..>1590
                     /locus_tag="PKV97_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNB07865.1"
                     /translation="MRRMAARSPSSLAPAFALAYALLVAYACLHPLTGWRDSGLPAFD
                     WLWAPWPKYFILEDLLFNILGYLPLGLLLAAALPAQWRFARKALVAALLAGLFSFGLE
                     ALQNYLPSRVASNLDLGGNAAGGLLGALAGARWGSALLGPQGRLQRWRARS"
BASE COUNT          220 a          545 c          591 g          234 t
ORIGIN      
        1 cgggcaggta gacctcgcac caggcgtgca ggtcggtgaa gtcgacctcg gtgcccgagg
       61 ggccgtcgag cgacttcacg tcgggcacga gctggatcag gtagccggac acgaagcgcg
      121 ccgccaggcc gaggtggcgc agcaactgca ccagcagcca ggccgagtcg cggcacgagc
      181 cgctcgccag cgtcagcgtc tcttccggcg tctgcacgcc cggctccagg cgcaccaggt
      241 agcggatgtc ctgctgcagg cgctggttaa gcgcgaccag gaaatccacg ctggcccgcg
      301 gcgcgcgcgg gatggattcg aggtagtcga tgaacttcgg cccgagcgcc gccgcgggcg
      361 ccttcgccag atagggcgcg agctcgacgc gcagggcctc ctcgtaggca aaggggatct
      421 tctccgccgc cggctcgaga aagaagtcga aggggttgat gaccgacagc tcggcgacca
      481 ggtcgacctc gatacgcagc tcggtggtct tgtccgggaa gacgaggcgg gcgaggtagt
      541 tcgactgcgg gtcctgctgc cagttgatga agtggccggc gggctcgacc ttcagcgcgt
      601 aggcatggat gggcgtgcgg cagtgcggcg ccgggcgcag gcgcacgacc tgcggcgaca
      661 gcgcgaccgg gcggtcgtag cggtaacggg tgacgtggtt gagcttgacg tggatggaca
      721 cggcggcctc gcagggagcg tagtggggcg acaccgggtg tcagcaagaa gcgtgcaagc
      781 ccgcgcgacg cctggcgtcc gccgtttccg ctggcactgc cccttccgtg caggcgcagt
      841 cgcgcaccat ttgcgcccgg agcgcaccag cgcggtgcgg caccagcgct ccaccgccgg
      901 aatcgcgact tgcgtcgcta cggcgaagag gcctcggagg cgtggcgcat tctcgtggga
      961 gcggcgcaag ccgcgatcgc agcctcgcgg caggcagggg cgcttgtcgc agtgccgccg
     1021 ccggagtcgc cacttgcatc gcccctgcgg aggaccctgt agcgtggcac cggagctcga
     1081 gcgcatggac accccgtccc ggggtgctgc gatccggccc agctggcaca atgcggcgca
     1141 tggccgcacg gtcgccctcc tccctcgctc ccgcgttcgc gctcgcctat gcgctgctgg
     1201 tcgcctatgc ctgcctgcat ccgctgaccg gctggcgcga cagcggcttg ccggcgttcg
     1261 actggctgtg ggcgccatgg ccgaagtact tcatcctcga agatctcctc ttcaacatcc
     1321 tcggctacct gccgctcggg ctgctgctgg cggcggcgct gccggcgcag tggcgcttcg
     1381 cgcgcaaggc gctcgtcgcc gcgctgctcg ccgggctgtt cagcttcggc ctggaggcgc
     1441 tgcagaacta cctgcccagc cgggtggcga gcaatctcga cctcggcggc aacgcggccg
     1501 gcggcctgct cggcgcactc gccggcgcgc gctggggcag tgcgctgctc gggccgcagg
     1561 ggcggctgca gcgctggcgc gcgcgcagcg
//
LOCUS       DAOQNN010000637         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_73530_length_1589_cov_8.392438, whole genome shotgun sequence.
ACCESSION   DAOQNN010000637 DAOQNN010000000
VERSION     DAOQNN010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73530_length_1589_cov_8.392438"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..778
                     /gene="xpt"
                     /locus_tag="PKV97_18160"
     CDS             176..778
                     /gene="xpt"
                     /locus_tag="PKV97_18160"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNB07866.1"
                     /translation="MLPDPDLIRPYGPLVRRIEAEATVIGDQILKIDHFLNHRIEPSF
                     ITEMGQELAKRLARFEPSVVLTAEASGIAPGLVVAQALDVPLVYAKKYAPEVESPAIA
                     RVIPSPTKGGETKLVLSQRYIVPTDRVVIVDDFLSNGRTAVALVEMAREAGAEVLCAS
                     FIVEKRFKHGHAAIEALGVPVCTLAQVEALENGHVRLTQP"
     gene            863..>1589
                     /locus_tag="PKV97_18165"
     CDS             863..>1589
                     /locus_tag="PKV97_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB07867.1"
                     /translation="MSSFDTQFEAFAEQVQQELREGVTSFPTVFDLSVRVQKLADAPE
                     TSLAQIAGAVQTEPLLAAKVIRMANTLTLNPYRGQITSLSDAIQRIGLSTLRCLAFAV
                     AAEQLARDHRSRQMRLIASGLWMHSIDVAAWSCAFAHHLKRPDPDAAMLAGLMLDIGQ
                     FYLLARAARYPALEGDMGRFAEVVTTWDEPVRDAVLEALEMPERIITACAADPFANTE
                     WPPTALGDVVFLATLAAESPNPFD"
BASE COUNT          252 a          616 c          494 g          227 t
ORIGIN      
        1 ggagcgccgg cgacgggccg cgcgcgcggg ccgcgagcgg ccgcccggcg ccgccaccgg
       61 atcgaagccg cggtcacgat cgagcgcgag gcttggaggt caagcacgct gccttcggct
      121 agaatccgcc cgtcctgccc ccggcccggg ggcgttcccg tttacggacc cgcgcatgct
      181 tcccgatccc gacctcatcc gcccctacgg cccgctcgtg cgccgtatcg aagccgaagc
      241 caccgtcatc ggcgaccaga tcctcaagat cgaccacttc ctcaaccacc gcatcgaacc
      301 cagcttcatc accgagatgg gccaggagct cgcgaagcgc ctggcacgct tcgaacccag
      361 cgtggtgctt accgccgagg ccagcggcat cgcccccgga ctggtggtcg cccaggccct
      421 cgacgtgccg ctggtgtatg cgaagaagta cgcgcccgag gtcgaatccc ccgcgatcgc
      481 gcgcgtgatc ccctcgccca ccaagggcgg cgagaccaag ctcgtgctgt cgcagcgcta
      541 catcgtgccc accgaccgcg tcgtcatcgt cgacgatttc ctctccaacg gccgcaccgc
      601 ggtggccctg gtcgagatgg cgcgcgaggc cggcgccgag gtgctgtgcg cgagcttcat
      661 cgtcgagaag cgcttcaagc acggccacgc cgccatcgag gccctcggcg tgccggtgtg
      721 cacgctcgcc caggtcgagg cgctggagaa cggccacgtc cgcctcaccc agccctgaag
      781 cgacagtcgt tcgtgggata tgcacgcgca cgccggccag catgcgcata gactgcgacg
      841 atcacacgaa cggaaccggc ccatgagctc tttcgacacc caattcgaag ccttcgccga
      901 acaggtgcag caggagctgc gcgaaggcgt gacgagcttt cccaccgtat tcgacctgtc
      961 ggtgcgcgtc cagaagctgg ctgacgcccc ggagacctcc ctcgcgcaga tcgcgggcgc
     1021 ggtgcagacc gagcccctgc ttgcggccaa ggtcatccgc atggccaaca cgctgacgct
     1081 gaacccctac cgcggccaga tcaccagcct gagcgatgcg atccagcgca tcggcctgtc
     1141 cacgctgcgc tgcctcgcct tcgcggtcgc cgccgagcag ctcgcgcgcg accaccgctc
     1201 gcgccagatg cgcctgattg ccagcggcct gtggatgcac tcgatcgacg tggcggcgtg
     1261 gtcctgcgcc ttcgcacacc acctcaagcg ccccgacccc gatgccgcga tgctcgccgg
     1321 cctgatgctc gacatcggcc agttctacct gcttgcgcgc gcggcgcgct acccggcgct
     1381 ggaaggggac atgggccgct tcgccgaggt ggtcaccacc tgggacgagc cggtgcgcga
     1441 cgccgtgctc gaagccctgg agatgcccga gcgcatcatc accgcctgcg ccgccgaccc
     1501 cttcgccaac accgagtggc caccgacggc gctcggcgac gtggtcttcc tcgccaccct
     1561 cgcggccgaa tcgcccaacc ccttcgaca
//
LOCUS       DAOQNN010000638         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_74403_length_1579_cov_11.461942, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000638 DAOQNN010000000
VERSION     DAOQNN010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74403_length_1579_cov_11.461942"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PKV97_18170"
     CDS             <1..872
                     /locus_tag="PKV97_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07868.1"
                     /translation="LGRSYDSFESRWFGYKRAKDAVNAHKTRISRIAFPHAFGEITSL
                     MRDVTLALNPKDWFPIKDPIVVPVYVDLPPEARKKYREMERDMLTHIGGHDIEAFAAS
                     GKVLKMLQLSNGACYTGSDEQIERDVSHWVEAHEEKLDALESIIEENDGEPILVAYHF
                     KPDLARLKKRFPHGRHINTKADEDAFKRGEIELAFVHPQSIGHGVDGFQNVCHIIVFF
                     ALAYDLDPHDQIIERVGPMRQMQAGFDREVLVYQIIARDTIDEAVAERLIEKRGVLDA
                     TMDALALKPKDIA"
     gene            869..1165
                     /locus_tag="PKV97_18175"
     CDS             869..1165
                     /locus_tag="PKV97_18175"
                     /inference="COORDINATES: protein motif:HMM:NF025717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HNB07869.1"
                     /translation="MKVYLSGPMTGIPESNYPAFHATAAKLRAAGYEVVNPAEVNPDS
                     SLSWQQCLREDIKHLCDCEALALMPGWQNSQGAHLEMHIAHRLGIKIHLVEELL"
     gene            1162..1440
                     /locus_tag="PKV97_18180"
     CDS             1162..1440
                     /locus_tag="PKV97_18180"
                     /inference="COORDINATES: protein motif:HMM:NF040395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6378 domain-containing protein"
                     /protein_id="HNB07870.1"
                     /translation="MTDSITQTLTDRGNRYGPFIGHAEVTQRLKRVMAEELAKRGKAL
                     ADDQWEALEMIAHKIGRIINGDPDYDDSWVDIAGYAKLVSDRLQGTVR"
BASE COUNT          314 a          525 c          476 g          264 t
ORIGIN      
        1 ggctgggccg gtcctacgac tcattcgaat cgcgctggtt cggctacaag cgggccaagg
       61 acgcggtgaa cgcgcacaag actcgcatct cgcgcatcgc cttcccgcat gccttcggcg
      121 agatcaccag cctgatgcgc gacgtgacgc tggccctcaa cccgaaggac tggttcccga
      181 tcaaggatcc gatcgtcgtg ccggtgtacg tcgatctgcc gcccgaggcg cgcaagaagt
      241 accgcgagat ggagcgtgac atgctcacgc acatcggcgg ccacgacatt gaggctttcg
      301 cggcgtcggg caaggtgtta aaaatgctcc agctcagcaa tggagcgtgc tacaccggct
      361 ccgacgagca gatcgagcgc gacgtgtcgc actgggtcga ggcccacgaa gagaaactcg
      421 acgcgttgga atcgatcatc gaagagaacg acggcgagcc gatccttgtc gcctatcact
      481 tcaagcccga cctggcgcgg ctcaagaagc gcttcccgca cggccgccac atcaacacca
      541 aggccgacga ggatgcgttc aagcgcggcg agatcgagct cgccttcgtg cacccgcaga
      601 gcattggcca cggggtcgac ggcttccaga acgtgtgcca catcatcgtc ttcttcgcgc
      661 tcgcctacga cttggaccca cacgaccaga tcatcgagcg ggtcgggcca atgcggcaga
      721 tgcaggcagg ttttgaccgc gaggtgttgg tctaccagat catcgcccgc gacactatcg
      781 acgaggccgt tgcggaacgg ctcatcgaga agcgtggtgt gctcgacgcc accatggacg
      841 ctctggctct taaaccgaag gacatcgcgt gaaggtctac ttgtccggcc cgatgacggg
      901 cataccggag tccaactacc cggcgtttca cgccacggcc gccaagctgc gcgccgccgg
      961 ctacgaggtc gtcaaccccg ccgaggtgaa ccccgactcg tcgctgtcgt ggcagcagtg
     1021 cctgcgcgaa gacatcaagc acctgtgtga ttgcgaggcg ctggcgctca tgcccggctg
     1081 gcagaactcg cagggcgcgc atctggagat gcacatcgcc catcgactgg gcatcaagat
     1141 ccatcttgtg gaggaacttc tgtgaccgac agcatcaccc aaaccctcac cgaccgcggc
     1201 aaccgctacg gccccttcat cgggcacgcc gaggtcaccc agcgtctcaa gcgcgtgatg
     1261 gccgaggaac tcgccaaacg cggcaaggcc ctggctgacg accagtggga ggcgctggag
     1321 atgatcgcgc acaagatcgg ccgcatcatc aacggcgacc cggactacga cgactcgtgg
     1381 gtggacatcg ccggctacgc caaactggtg tcggatcgct tgcaaggtac cgtccgctag
     1441 gcgtatcatc tgctccacaa ctctcaggag tctgccatgc gtgaccacct tctcggctgt
     1501 caagccttcc tgccgcccga acccgatccg caggatcgca cgattcatct gcgccggcgt
     1561 tttccggtcg gcgtcccgg
//
LOCUS       DAOQNN010000639         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_74404_length_1579_cov_10.412073, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000639 DAOQNN010000000
VERSION     DAOQNN010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74404_length_1579_cov_10.412073"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PKV97_18185"
     CDS             <1..781
                     /locus_tag="PKV97_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNB07871.1"
                     /translation="GIAEQHAVTFAAGLACEGFVPVVAIYSTFLQRAYDQLIHDVALQ
                     NLPVVFAIDRGGLVGADGATHHGAFDLSFLSCVPNMVVMAPSDEDECRQMLYTAVQHA
                     GPSAVRYPRGGGNGTAPRAQMRALPIGKGEVVRRGRGIALLAFGSLLGVAREVAETLD
                     ATVANMRFVKPLDVELVAELATSHDLIVTLEENAVIGGAGAEVSRLVDGLATRPRVLR
                     LGLPDHFIDHGDQAQLLASVGLDQDGILASIARATGAPERA"
     gene            896..>1579
                     /gene="folE2"
                     /locus_tag="PKV97_18190"
     CDS             896..>1579
                     /gene="folE2"
                     /locus_tag="PKV97_18190"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNB07872.1"
                     /translation="MNTPNAIAIPDVQSSADSRRIAIDKVGIKSIRHPIKVADKRGGV
                     QHTVASFNMYVGLPHNFKGTHMSRFIEILNGREREISVENFEPMLRTMVERLEAETGH
                     VEMSFPYFINKTAPVSGVQSLMDYEVCFIGSIGEGGAYEFTMKVVVPVTSLCPCSKKI
                     SAYGAHNQRSHVTVTAVLNDHLWIEEMVQMVEAQASCEVFGLLKRPDEKWVTERAYDN
                     PKFVEDMVRD"
BASE COUNT          278 a          571 c          495 g          235 t
ORIGIN      
        1 cggcatcgcc gagcagcacg cggtcacctt cgccgccggg ctggcctgcg agggcttcgt
       61 gccggtggtg gcgatctact cgaccttcct tcagcgcgcc tacgaccagc tcatccacga
      121 cgtcgcgctg cagaacctgc cggtcgtgtt cgcgatcgac cgcggcggcc tggtgggcgc
      181 cgacggcgcg acccaccacg gcgccttcga cctctccttc ctcagctgcg tgcccaacat
      241 ggtggtgatg gcgccctcag acgaggacga atgccgccag atgctctaca ccgcggtgca
      301 gcatgcgggc ccgagcgcgg tgcgctatcc gcgtggcggc ggcaacggca ccgcgccccg
      361 ggcacagatg cgcgccctgc cgatcggcaa gggcgaggtc gtgcgccgtg gccgcggcat
      421 cgccctgctc gccttcggca gcctgctcgg agtggcgcgc gaggtcgccg agacgctcga
      481 cgccacggtg gccaacatgc gcttcgtcaa gccgctcgac gtcgagctgg tggccgaact
      541 cgccaccagc cacgacctca tcgtcaccct cgaggagaac gcggtgatcg gcggcgccgg
      601 cgccgaggtc tcacggctcg tcgacggcct cgccacgcgc ccacgcgtgc tgcgcctggg
      661 cctgcccgac cacttcatcg accacggcga ccaagcacaa ctgctcgcct cggtcggcct
      721 cgatcaggac ggcatcctcg ccagcatcgc acgcgcgacc ggagccccgg agcgcgccta
      781 aaggttgagc atttttgtcg ggaaagataa gatcgccagc aaagtgcaac acgattgcac
      841 tttgctggcg gctccggcac gcccattccc ccgaccaccc gcagggaacc tgcccatgaa
      901 cacccccaac gcaatcgcca tcccggacgt gcagagcagc gccgacagcc gccggatcgc
      961 gatcgacaag gtcggcatca agtcgatccg ccaccccatc aaggtcgccg acaagcgcgg
     1021 cggcgtgcag cacacggtgg ccagcttcaa catgtacgtc ggtctgcccc acaacttcaa
     1081 gggcacccac atgtcgcgct tcatcgagat cctcaacggc cgcgagcgcg agatctcggt
     1141 ggagaacttc gagcccatgc tgcgcaccat ggtcgagcgc ctcgaggccg agaccggcca
     1201 cgtcgagatg agtttcccct acttcatcaa caagacggcg ccggtgtccg gcgtgcagag
     1261 cctgatggac tatgaggtct gcttcatcgg cagcatcggc gagggcggcg cctacgagtt
     1321 caccatgaag gtcgtggtgc cggtgaccag cctgtgccca tgctcgaaaa agatctccgc
     1381 gtacggcgcg cacaaccagc gctctcacgt caccgtcacc gcggtgctca acgaccacct
     1441 gtggatcgag gagatggtgc agatggtcga ggcccaggcc tcgtgcgagg tcttcggcct
     1501 gctcaagcgc cccgacgaga agtgggtcac cgagcgcgcc tacgacaacc ccaagttcgt
     1561 cgaggacatg gtgcgcgac
//
LOCUS       DAOQNN010000640         1575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_74788_length_1575_cov_5.106579, whole genome shotgun sequence.
ACCESSION   DAOQNN010000640 DAOQNN010000000
VERSION     DAOQNN010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74788_length_1575_cov_5.106579"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1575
                     /locus_tag="PKV97_18195"
     CDS             <1..>1575
                     /locus_tag="PKV97_18195"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB07873.1"
                     /translation="GLVERRQVLRASLLAALAGEHSLLIGPPGTAKSALARRIHLAFR
                     DARYFERLLTRFTVPEELFGPLSIRALEEDRYERHVAGFLPDAAVAFIDEVFKANSAI
                     LNALLTLLNEREFDNGAGRLRCPLISVVGATNQVPEDEVAEAFFDRFLLRLTVAPVSA
                     EGFRWLLGEDRPGSPEVLAPPSGLQLDEADRAALAQAAAACALPAAVLDRLGELRAWL
                     AGRGVYVSDRRWVKLAHLLRVAAASEGRAAASEWDLGLAPCCVAADEPTRLAVADWLA
                     ARLGIREVWSPPRITRVVEAFEAQLATEREARDLDYDERGRLRFSAIDPVATQAGELA
                     GSIGDAKGGAAALRMSAARMRRYGSLHIGARTAQLDGVLARLAAYRGELGAQRATLEA
                     WADQALWADPAFVACARSGLDAVDAVLAGLEARARSARAGFEALPRLEQAGAAPAPVE
                     HDHLAPRLQQRVGNQGAADAGTDHDHVGPDARAERLARYPGRTAPEPDGRAGPQVALL
                     RGAHPSSRTIGRGGSPA"
BASE COUNT          176 a          571 c          609 g          219 t
ORIGIN      
        1 ggcctggtcg aacgccggca ggtgctgcgc gcgagcctgc tcgccgcgct cgcgggcgaa
       61 cactcgctgc tgatcgggcc gcccggcacg gccaagagcg cgctcgcgcg ccgcatccac
      121 ctggcctttc gcgatgcgcg ctatttcgag cgcctgctca cccgcttcac cgtgccggag
      181 gagctcttcg gcccgctgtc gatccgcgcg ctcgaggaag accgctacga gcgccacgtg
      241 gcgggcttcc tgcccgatgc ggcggtggcc ttcatcgacg aggtcttcaa ggccaacagc
      301 gcgatcctca acgcgctgct gacactcctc aacgagcgcg agttcgacaa cggcgcgggc
      361 cgcctgcgct gtccgctgat cagcgtggtg ggggcgacca accaggtccc cgaggacgag
      421 gtggcggagg ccttcttcga ccgcttcctg ctgcgcctga ccgtggcacc ggtgagcgcg
      481 gaggggtttc ggtggctgct cggcgaggat cgcccggggt cgcccgaggt actcgcgccg
      541 ccttccggac tgcagctcga cgaggccgac cgcgcggcgc tggcgcaggc tgccgccgct
      601 tgcgcactgc ctgcggcggt gctcgaccgc ctcggcgagc tgcgggcctg gctggcgggg
      661 cgtggggtgt atgtatccga ccggcgctgg gtcaagctcg cccacctgct gcgcgtggcc
      721 gcggcgagcg agggcagggc ggcggcgagc gagtgggatc tgggcctggc gccgtgttgc
      781 gtcgctgccg acgagcccac gcggctggcg gtggcggact ggctggcggc gcgtctgggg
      841 atccgcgagg tctggtcgcc gccgcgcatc acgcgggtgg tggaggcctt cgaggctcag
      901 ctcgccaccg agcgcgaggc tcgggatctc gactacgacg agcgtgggcg cttgcgcttc
      961 tcggcgatcg acccggtggc gacgcaggcc ggcgagctcg ccggctcgat cggcgacgcc
     1021 aagggcgggg cggcggcgct gcgcatgagc gccgcgcgca tgcggcgcta tggcagcctg
     1081 cacatcggtg cgcgcacggc ccagctcgac ggcgtgctcg cgcgcctggc ggcgtatcgc
     1141 ggcgaactcg gcgcgcagcg tgcgacgctc gaggcatggg cggaccaggc gctgtgggcg
     1201 gaccctgcct tcgtcgcctg cgcgcgttcc gggctggacg cggtggatgc ggtactggcc
     1261 gggctcgagg cccgcgcccg cagcgcgcgt gccggcttcg aggcgttgcc gcggctggag
     1321 caggcaggcg cggcgcccgc gccggtcgag cacgaccacc tcgcgccccg attgcagcag
     1381 cgcgtcggca accagggcgc cgctgatgcc ggcaccgacc acgaccacgt cggcccggat
     1441 gctcgcgcgg agcggctcgc tcggtaccct gggcgcaccg cgcccgagcc agacgggcgt
     1501 gccggtccgc aggtcgcgct cctgcgtggc gctcaccctt ccagccgcac catcggccgc
     1561 ggcggatcgc ccgct
//
LOCUS       DAOQNN010000641         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_74876_length_1574_cov_9.377222, whole genome shotgun sequence.
ACCESSION   DAOQNN010000641 DAOQNN010000000
VERSION     DAOQNN010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74876_length_1574_cov_9.377222"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    148..247
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            465..1349
                     /locus_tag="PKV97_18200"
     CDS             465..1349
                     /locus_tag="PKV97_18200"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HNB07874.1"
                     /translation="MADIGFIGLGIMGTPMAGNLIKGGHTVFTHTRGDTPAVLVEAGA
                     RPCASGAEVARQADIVITMVPDTPHVEDVLFSPGGVAAGLSKGKIVIDMSSISPVATK
                     DFAARINALGCEYLDAPVSGGEVGAKAATLTIMVGGSQAAFDKVKPIFDLMGKNITLV
                     GGNGDGQITKVANQIIVALNIEAVGEALLLAAKAGADPAKVRQALMGGFANSRILEVH
                     GERMVKRTFDPGFRIELHQKDLNLALTTARQLGVSLPNTATAQELFNACSAHGGAKWD
                     HSAMVRALEKMANFEIGQ"
     gene            1360..>1574
                     /locus_tag="PKV97_18205"
     CDS             1360..>1574
                     /locus_tag="PKV97_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HNB07875.1"
                     /translation="MRHLAIDVGSFRFVARMEEAAAPKTCAAFLELLPFSNQVIHSRW
                     SGEAVWVPLGEFDTGLGFENHTSHPSRG"
BASE COUNT          260 a          543 c          468 g          203 t
ORIGIN      
        1 gctacgaggg ctggatcggc tgcgaataca agcccgccgc cggcacccgc gaagggctgg
       61 gctggatcca ggcgctcgcc ggctgagcga ccttcgcgcc ttgcggcgct cctacaccga
      121 ccgacacggt cttgcgaaag cgccgtannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnaac cggcggcatc gcggttcgac acgctgcatt cgcgccttgc ggcgctccta
      301 caccgaccga cacggtcttg cgaaagcgcc gtaggagcgg cgcaagccgc gaacgggcgg
      361 tcggtctgca accggcggca tcgcggttcg acacgctgca ttcgcgcctt gcggcgctcc
      421 tacaggaaac aacccggctt caagcacatc caggagacaa gatcatggca gacatcggat
      481 tcatcggcct cggcatcatg ggcaccccca tggcaggcaa cctgatcaag ggcggccaca
      541 ccgtcttcac ccacacccgc ggcgacaccc cggccgtgct cgtcgaggcc ggcgcccgcc
      601 cctgcgcgag cggcgccgag gtcgcgcgcc aggcagacat cgtcatcacc atggtgcccg
      661 acaccccgca cgtcgaggac gtgctcttca gccccggagg cgtcgccgcc ggcctgtcga
      721 agggcaagat cgtgatcgac atgagctcga tctctccggt cgccaccaag gacttcgccg
      781 cccgcatcaa cgcgctcggc tgcgaatacc tcgacgcccc ggtctcgggc ggcgaggtcg
      841 gcgccaaggc ggcgacgctc accatcatgg tcggcggcag ccaggccgcc ttcgacaagg
      901 tgaagccgat cttcgatctg atgggcaaga acatcaccct ggtcggcggc aacggcgacg
      961 gacagatcac caaggtcgcc aaccagatca tcgtcgcgct caacatcgag gcggtgggcg
     1021 aggccctgct gctcgccgcc aaggccggcg ccgacccggc caaggtgcgc caggcgctga
     1081 tgggcggctt cgccaactcg cgcatcctcg aagtgcacgg cgagcgcatg gtcaagcgca
     1141 ccttcgaccc cggcttccgc atcgagctgc accagaagga cctcaacctc gcgctgacca
     1201 ccgcgcgcca gctcggcgtg tcgctgccca acaccgccac cgcgcaggag ctcttcaacg
     1261 cctgcagcgc gcacggcggc gcgaagtggg accactcggc gatggtgcgc gcgctggaga
     1321 agatggccaa cttcgagatc ggccagtaag gaggacgaca tgcgccatct cgccatcgac
     1381 gtcggctcct tccgcttcgt cgcccgcatg gaggaggctg ccgcacccaa gacctgcgcc
     1441 gccttcctcg agctgctgcc cttctccaac caggtgatcc actcgcgctg gagcggcgag
     1501 gcggtgtggg tgccgctggg cgaattcgac accgggctgg gcttcgagaa ccacaccagc
     1561 cacccctcgc gcgg
//
LOCUS       DAOQNN010000642         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_75151_length_1571_cov_5.298813, whole genome shotgun sequence.
ACCESSION   DAOQNN010000642 DAOQNN010000000
VERSION     DAOQNN010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75151_length_1571_cov_5.298813"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..>1571)
                     /locus_tag="PKV97_18210"
     CDS             complement(297..>1571)
                     /locus_tag="PKV97_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNB07876.1"
                     /translation="VRGQRAIAVGRDGRLSGPALAGALTAGIRAAGIDVIDIGCVPTP
                     LTYFAAFELGCASCVSVTGSHNPPAYNGLKIVLDGRTLHGEAIQDLRRRIAEDDLVEG
                     AGALRGADVRAAYLDRIVGDVELARPLKIVLDCGNGVAGAVAPELFRRLGCELVELFC
                     EVDGRFPNHHPDPSRPENLQDVIRALRETDAELGLAFDGDGDRLGVVCKDGEIVFPDR
                     QLMLFAADVLARVPGGEILYDVKCTRNLAPWIRAHGGRPTMWKTGHALLKAKLQETGA
                     ALAGEMSGHMFFKERWYGFDDGLYAGARLLEILSARADANAALKALPDATSTPELNLK
                     MQEGEPFELVRRLREAALATPSCFADAREVITLDGLRVEFAHGFGLARPSNTTPVVVL
                     RFEADDAPALAAIQAAFRRALDAAWPGLALPF"
BASE COUNT          207 a          533 c          609 g          222 t
ORIGIN      
        1 actcgtccat gagctgggcg aggatgccgg ggcaggacgg gatgtcgatg cccttgtggg
       61 cctgcttgct ctcgcgtgcg cgtgcctcgc gctgttcccg tgccgtcatc gactgcctcg
      121 ttcgcattgg atcaggtaaa aacaacctca tgattgtgcg ccgcgacgga cgcaagcggc
      181 gtgcattttt tcgcgttctc ccgccggttt tggggggcgc cggatggcgg gcgccatgcg
      241 gcagggtggt gggggtgggg ggcgatcgag ctccccctgg gcgcccgccc gcagcctcag
      301 aacggcagcg cgagcccggg ccaggccgcg tcgagcgcgc gccggaacgc cgcctggatc
      361 gcggcgagcg ccggagcgtc gtcggcctcg aagcgcagca ccaccaccgg cgtggtgttc
      421 gaggggcggg cgagcccgaa gccatgggcg aactccaccc gcaggccgtc gagcgtgatc
      481 acctcgcgcg cgtcggcgaa gcacgagggc gtggcgagcg cggcctcgcg cagccggcgc
      541 accagctcga agggctcgcc ctcctgcatc ttcaggttca gctccggggt gctcgtcgcg
      601 tcgggcagcg ccttcagcgc ggcgttggcg tcggcgcggg cggagaggat ctcgagcagg
      661 cgggcgcccg cgtagaggcc gtcgtcgaag ccgtaccagc gctccttgaa gaacatgtgg
      721 ccgctcatct cgccggcgag tgcggcgccg gtctcctgca gcttcgcctt gagcagcgcg
      781 tggccggtct tccacatcgt cggccggccg ccgtgcgcgc ggatccacgg cgccagattg
      841 cgggtgcact tgacgtcgta gaggatctcg ccgcccggta cgcgggcgag tacgtccgcg
      901 gcgaagagca tgagctggcg atccgggaag acgatctcgc cgtccttgca caccacgccg
      961 aggcggtcgc cgtcgccgtc gaaggccagg cccagctcgg cgtcggtctc gcgcagcgcg
     1021 cggatgacgt cctgcaggtt ctccggccgc gacgggtcgg ggtggtgatt gggaaagcgg
     1081 ccgtcgacct cgcagaacag ctcgacgagc tcgcagccca ggcggcggaa gagttcgggc
     1141 gcgacggcgc cggccacgcc gttgccgcag tcgagcacga tcttcagcgg gcgcgcgagc
     1201 tcgacgtcgc cgacgatgcg gtcgaggtag gcggcgcgca cgtcggcgcc gcgcagcgcg
     1261 ccggcaccct cgacgaggtc gtcctcggcg atgcgccggc gcaggtcctg gatcgcctcg
     1321 ccgtgcaggg tgcggccgtc gagcacgatc ttgaggccgt tgtaggcggg cgggttgtgg
     1381 ctgccggtga ccgacacgca ggaggcgcag ccgagctcga aggcggcgaa gtaggtgagc
     1441 ggggtgggca cgcagccgat gtcgatcacg tcgatgccgg cggcgcggat gcccgcggtc
     1501 agcgcgccgg ccagcgccgg gccggacagg cggccgtcgc ggcccaccgc gatcgcgcgc
     1561 tggccgcgca c
//
LOCUS       DAOQNN010000643         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_75414_length_1568_cov_9.811633, whole genome shotgun sequence.
ACCESSION   DAOQNN010000643 DAOQNN010000000
VERSION     DAOQNN010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75414_length_1568_cov_9.811633"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PKV97_18215"
     CDS             <1..544
                     /locus_tag="PKV97_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HNB07877.1"
                     /translation="PAWPAWPMVPACYGWLALDARGGWRLRGEIITHPGLLAFLDAHY
                     TGDAHGNWGVDNGPQRVYVELAAAPLILRLHPDGRLVAHTGREATTTGPVLVDADGCA
                     FLATDLGPAMIDDRDLALFAAELRQLEGTPAGDEDLLALIDGQSGPALRWREVDVAFC
                     ARAAIPGRLGFQPHPQAIGT"
     gene            698..782
                     /locus_tag="PKV97_18220"
     tRNA            698..782
                     /locus_tag="PKV97_18220"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:732..734,aa:Leu,seq:gag)
     gene            complement(905..>1568)
                     /locus_tag="PKV97_18225"
     CDS             complement(905..>1568)
                     /locus_tag="PKV97_18225"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304059.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNB07878.1"
                     /translation="FCVPSGNFGNICAGHIARQMGLPVAKLILATNENDVLDEFFRSG
                     VYRPRKAAETHVTSSPSMDISKASNFERFVFDLVGRDPSKVAALWAEVDAGRAFDLSG
                     TDEFARIGAFGFVSGASSHADRLATIRKVYERYGVMIDTHTADGVKVAWERAADVPAG
                     VPVLVLETALPVKFAETIVEALGREPERPADLEGIEKLPQRVEVMAPDADAIKRFIAQ
                     RT"
BASE COUNT          227 a          576 c          534 g          231 t
ORIGIN      
        1 gcccgcctgg cccgcttggc ccatggtgcc ggcctgctac ggctggctcg cgctcgacgc
       61 ccgcggcggc tggcggctgc gcggcgagat catcacccac cccggactgc tcgccttcct
      121 cgacgcccac tacaccggcg acgcgcacgg caactggggg gtcgataacg gcccgcagcg
      181 cgtctacgtg gaactcgctg cggcaccgct gatcctgcgc ctgcatcccg acggccgcct
      241 ggtcgcccac accggacgcg aggcgaccac gaccggaccg gtgctggtcg acgcggacgg
      301 ttgcgccttc ctcgccaccg acctgggtcc ggcgatgatc gacgaccgcg acctcgcgct
      361 tttcgccgcc gaactgcgcc agctcgaggg cacgcccgcc ggcgacgagg acctgctcgc
      421 actgatcgac gggcagtccg gcccggcact gcgctggcgc gaggtcgacg tggccttctg
      481 cgcgcgtgcg gcaatccccg ggcgcctcgg cttccagccc cacccgcaag ccatcggcac
      541 ctgatccgca gcgccatcgt ccgtcgccgc gacgacgccc gcgctcggcg ctcgcatcag
      601 acacggcagg tgttgacccc cacccggctt cacggctatg atccgcgccc ttgctcgatg
      661 cggccggcgc cacagcgcag aacaccgcag cagcgaagcc ttggtggtga aattggtaga
      721 cacgctatct tgagggggta gtggcgaaag ccgtgcgagt tcgagtctcg cccaaggcac
      781 cagattacgg ccccgccgac ggcggcggcg tccgacgaag aaaagcccgc gaatcgctcg
      841 cgggcttttc ttcgtttacg tcccggcgtc cccgccggcc cgctcgccga cggggacgcg
      901 gcgatcaggt gcgctgggcg atgaagcgct tgatcgcgtc ggcgtcgggc gccatcacct
      961 cgacgcgctg cggcagcttc tcgatgcctt cgaggtctgc gggacgctcc ggctcgcgcc
     1021 caagggcctc gacgatggtc tcggcgaact tcaccggcag cgcggtctcc agcaccagca
     1081 ccggcacgcc cgcaggtacg tcggcggcgc gctcccaggc caccttgacg ccgtcggcgg
     1141 tgtgggtgtc gatcatcacg ccgtagcgct cgtacacctt gcggatggtc gccaggcgat
     1201 cggcatggct gctcgcgcca gacacgaagc cgaacgcgcc gatgcgggcg aattcgtccg
     1261 tgccggacag gtcgaaggcg cgcccggcgt cgacctcggc ccacagcgcg gccaccttgc
     1321 tcgggtcgcg gccgaccagg tcgaagacga agcgctcgaa gttcgaggcc ttggagatgt
     1381 ccatcgacgg gctggaggtc acgtgcgtct cggccgcctt gcgcgggcga tacacgccgc
     1441 tgcggaagaa ctcgtcgagc acgtcgttct cgttggtggc gaggatcagc ttcgccaccg
     1501 gcaggcccat ctggcgggcg atgtggccgg cgcagatgtt gccgaagttg cccgagggca
     1561 cgcagaag
//
LOCUS       DAOQNN010000644         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_75421_length_1568_cov_5.535360, whole genome shotgun sequence.
ACCESSION   DAOQNN010000644 DAOQNN010000000
VERSION     DAOQNN010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75421_length_1568_cov_5.535360"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1568
                     /locus_tag="PKV97_18230"
     CDS             <1..>1568
                     /locus_tag="PKV97_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNB07879.1"
                     /translation="RYGGKLPALEQARDHREVLQAMPPMASPAGSFLASGSGWYPRPA
                     DLFAYEVDLVVRGGQPALVAGRLAHEQRPAAAGEPYRARFIFEHPADGIDLMAGPWVV
                     RERLATQADGRPLRLRTYFPAALDGEAGLAEAYLADSQRYIERYSAQIGAYPFTEFSV
                     VASPLPTGFGMPTLTYIGEQVLRLPFIRASSLGHEVLHNWWGNGVLVDYARGNWSEGL
                     TTFMADYAYKTEASAAAAREMRLSWLRDFAALPADSHAPLADFRSRTHGAAAAVGYGK
                     AAMVFVMLQDEIGEEAFARGIRLFWEHERFRIAGWDALRAAFEEASGRSLQGFFRQWL
                     ELPGGPAPRIERARLVEQAGQGARVRVSLAQPGPVHALRLPLQLVAGDRVETRVVEFA
                     AGRTDVELTAGFVPEGVALDPELRLWRLPEAAQLPPILRQWIVAAAPRLVLADAQTEA
                     GGAGSGVGSGASGGTPRGADSAFAEAAQALAARLFERTPTLAGLPALGAEGGPVLLVG
                     SEPAVAAVLVGAGLA"
BASE COUNT          186 a          561 c          600 g          221 t
ORIGIN      
        1 cgctacggcg gcaagctgcc cgcgctcgag caggcacgcg accaccgcga ggtgctgcag
       61 gccatgccgc cgatggcctc gccagcgggc agcttcctcg ccagtggcag cggctggtat
      121 ccgcgtccgg ccgacctctt cgcctacgag gtggacctcg tggtccgtgg cggccagccg
      181 gcgctggtcg ccggacggct ggcgcacgag cagcgccccg ccgcggcagg cgagccctac
      241 cgcgcgcgct tcatctttga acatccggca gacggcatcg acctgatggc cgggccctgg
      301 gtggtgcgcg aacgcctcgc cacccaggcc gacggccggc cgctgcgcct gcgcacctac
      361 tttcccgccg cgctcgacgg cgaggccggg ctggccgagg cctacctcgc cgacagccag
      421 cgctacatcg agcgctactc ggcacagatc ggcgcctacc ccttcaccga gttctcggtg
      481 gtggcgagcc cgctgccgac cggcttcggc atgcccacgc tgacctacat cggcgagcag
      541 gtgctgcgcc tgcccttcat ccgcgcgagc tcgctcggcc acgaggtgct gcacaactgg
      601 tggggcaacg gcgtgctggt cgactacgcg cgtggcaact ggtcggaggg cctgaccacc
      661 ttcatggccg actacgccta caagaccgag gcgtcggccg ccgcggcgcg cgagatgcgc
      721 ctgagctggc tgcgcgactt cgccgcgctg ccggcggatt cgcacgcacc gctcgccgat
      781 ttccgctcgc gcacccacgg cgccgcggcc gcggtgggtt acggcaaggc ggcgatggtc
      841 ttcgtgatgc tgcaggacga gatcggcgag gaggccttcg cgcgcggcat ccgcctgttc
      901 tgggagcatg agcgcttccg catcgccggc tgggacgcgc tgcgcgccgc cttcgaggag
      961 gcgtccggac gttcgctgca gggcttcttc cgccagtggc tggaactgcc cggggggccg
     1021 gcgccgcgca tcgagcgggc acgcttggtg gagcaggccg ggcagggcgc gcgcgtgcgc
     1081 gtgagcctgg cgcaaccggg accggtgcac gccttgcggc tgccgctaca gctcgtggcg
     1141 ggggatcggg tagagacccg ggtcgtcgag ttcgccgcgg gccgtaccga tgtggagctg
     1201 accgccggct tcgtgcccga gggcgtggcc ctcgacccgg agctgcgtct gtggcgcctg
     1261 cccgaggcgg cgcaactgcc gcccatcctg cgccagtgga tcgtcgctgc ggcgccgcgg
     1321 ctggtgcttg ccgacgcgca gacggaggcg ggcggggcgg ggagtggcgt agggagtggc
     1381 gcaagcggtg gcacgccgag aggggcggac agtgcattcg ccgaggcggc gcaggcgctc
     1441 gccgcccgcc tgttcgagcg cacgccgacg ctggcaggtc tcccagcctt gggcgcagag
     1501 ggcgggccgg tgctgctggt gggcagcgag cccgcggtgg ccgcggtgct cgtcggtgcc
     1561 ggcctggc
//
LOCUS       DAOQNN010000645         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_75848_length_1563_cov_8.122679, whole genome shotgun sequence.
ACCESSION   DAOQNN010000645 DAOQNN010000000
VERSION     DAOQNN010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75848_length_1563_cov_8.122679"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PKV97_18235"
     CDS             <1..1092
                     /locus_tag="PKV97_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNB07880.1"
                     /translation="FGIAAIARLLALRAQANAALAAERGVALAKQQAVSAHILRDMQD
                     GVIVVGARGRVLHANAQALEMLGLTRAGDGALDGLALADLDAGLEDMLQPAVGEDGRL
                     MRVGAAGRLLRCRRVQGDPGSAAEGDTLLYLTDYEEIQRQLQQIKLAALGRLTASMAH
                     EIRNPLSAVTQAAELLGEEKRADMQARLVRIINDNARRIERMIRDVLALGRREQTMPE
                     ALPLAAFVREILDARVFREPAEAQLYELAIDPALTLAMDRAHLHQILDNLLSNAARHA
                     SGAPGAVRLWAEALPHAKVVLHVQDDGPGFDEAMRAHLFEPFFTTHPKGTGLGLYIAR
                     ELAEANDATLALAPSTPGADFILTASQQP"
     gene            1089..>1563
                     /locus_tag="PKV97_18240"
     CDS             1089..>1563
                     /locus_tag="PKV97_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB07881.1"
                     /translation="MTQVDRRNRPRAADVLVVDDEDDIRELLELSLMRMGLACDTAGS
                     VGAARALLAERSYRLCLTDMRLPDGDGLELVEYIQTLRPGLPVAVITAFGSIETAIRA
                     LKLGAFDFVTKPVELKALRELVQHALKLHAVEPVEHGAASAERSLIGHSAAFVQLR"
BASE COUNT          222 a          580 c          550 g          211 t
ORIGIN      
        1 ttcggcatcg cggcgatcgc gcgcctgctc gcgctgcgcg cccaggccaa cgccgcgcta
       61 gcggccgagc gcggcgtggc gctcgccaag cagcaggcgg tgagcgccca catcctgcgc
      121 gacatgcagg acggcgtgat cgtggtcggc gcgcgcggcc gcgtcctcca cgccaacgcg
      181 caggcgcttg agatgctcgg cctgacccgt gccggcgacg gggcgctcga cggactcgcg
      241 ctcgccgatc tcgacgccgg gctggaggac atgctgcagc cggcggtggg cgaggacggg
      301 cgtctgatgc gggtgggtgc ggccggccgc ctgctgcgct gccgccgcgt gcagggcgac
      361 ccgggtagcg cggccgaggg cgacaccctg ctgtatctca ccgactacga ggagatccag
      421 cgccagctgc agcagatcaa gctcgccgcg ctcggccgcc tcaccgcaag catggcgcac
      481 gagatccgca acccgctgtc ggcggtgacc caggccgccg agctgctcgg cgaggaaaaa
      541 cgcgccgaca tgcaggcgcg cctggtgcgc atcatcaacg ataatgcgcg ccgcatcgag
      601 cgcatgatcc gcgacgtgct cgccctcggc cggcgcgagc agaccatgcc cgaggccctg
      661 ccgctggccg ccttcgtgcg cgagatcctc gatgcgcgcg tgttccgcga gcccgccgag
      721 gcgcagctgt acgagcttgc gatcgacccc gcgctgaccc tggcgatgga ccgcgcccac
      781 ctgcaccaga tcctcgacaa cctgctgtcg aacgcggccc gccatgcctc gggcgcgccc
      841 ggcgcggtgc gcctgtgggc ggaggccctg ccgcacgcca aggtcgtgct gcacgtgcag
      901 gacgacggtc cgggcttcga cgaggcgatg cgggcgcacc tgttcgaacc tttcttcacc
      961 acccatccca agggtaccgg cctcggactg tacatcgcgc gcgagctggc cgaggccaac
     1021 gacgccaccc tggcgctcgc cccgagtacc cccggtgccg acttcatcct caccgcgagc
     1081 cagcagccat gacccaggtc gaccgccgca accggccccg tgccgccgac gtcctcgtcg
     1141 tcgatgacga ggacgacatc cgggaactgc tcgagctctc gctgatgcgc atgggactgg
     1201 cctgcgacac ggcgggctcg gtgggcgcgg cgcgtgcgct gctggccgag cgcagctacc
     1261 ggctgtgcct gaccgacatg cgcctgcccg acggcgacgg gctggagctg gtcgagtaca
     1321 tccagacgct gcggccgggc ctgccggtgg cggtgatcac cgccttcggc agcatcgaga
     1381 ccgcgatccg cgcgctcaag ctgggtgcct tcgacttcgt caccaagccg gtcgagctca
     1441 aggccttgcg cgaactcgtg cagcacgcgc tcaagctgca cgcggtggag ccggtcgagc
     1501 atggtgcggc gtcggcggag cgcagcctga tcggccactc ggcggccttc gtgcagctgc
     1561 gcg
//
LOCUS       DAOQNN010000646         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_76191_length_1559_cov_13.138963, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000646 DAOQNN010000000
VERSION     DAOQNN010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76191_length_1559_cov_13.138963"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>1559
                     /locus_tag="PKV97_18245"
     CDS             join(17..455,455..>1559)
                     /locus_tag="PKV97_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612229.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNB07882.1"
                     /translation="MARYGQAFKDRAVARLLPPESAPLEAVAREIGLGAATLERWRAH
                     ALAKPAGQRAWTAVARLEAVIATAALDETARSAWCREHGVYPQQLGQWRDNATQALAE
                     PEEARASPQQTRHERRRIKELERELRRKDRALAETAALLVLSKKGRGDLQQGRGRMIG
                     LEDRQALARDIEAARQAGARLRLACEIAGIDVRTLQRWKADDGLVAGDGRPEAARPTP
                     AHALSPAEREYIVHVANQPRFADMPPARIVPALADEGVYVASESSFQRVLRAHGQTRH
                     RGRAKAPRKSRPPTTHVATAPRQLWCWDMTYLPAEVIGRWFYLYLILDVYSRKIVGFE
                     VHDSDDGDHAAHLLKRTALAEGIHAMLNKPVLHGDNGSTLKATSVLAMLHWLGVKPSY
                     SRPRVSDDNAYVESLFRTAKYRPEFPAKGFADLDQARAWAAQFVHWYNHDHRHSGIRY
                     VSPAQRHAGADTAILAARHELYCRARERNPRRWSGPTRNWTPVTVVTLNPERDSVVET
                     HSRTLD"
BASE COUNT          259 a          566 c          543 g          191 t
ORIGIN      
        1 gatgacagag gacgaattgg ctcgatacgg acaagcattc aaggaccggg cagtggcacg
       61 gctgctgccg cccgagagcg cgccgctgga ggcggtggcg cgcgagatcg ggttgggcgc
      121 ggcgacgctc gagcgctggc gcgcccacgc gctggccaag cctgcggggc aacgcgcctg
      181 gacggcggtg gctcggctgg aggcggtgat cgccacggcc gcgctggacg agacggcgcg
      241 cagcgcgtgg tgccgcgagc acggcgtgta cccgcagcag ctcgggcagt ggcgcgacaa
      301 cgccacccag gcgctggccg agcccgagga ggcgcgcgcc agcccgcagc agacccggca
      361 cgagcgccga cgcatcaagg agctcgagcg cgagctgcgg cgcaaggacc gggccctggc
      421 cgagaccgca gcgctgctgg tgctgtcaaa aaaagtcgcg gcgatcttca acaagggcga
      481 ggacgaatga tcggcctcga agatcgccaa gccctggccc gagacatcga agcggctcgc
      541 caggccggcg cgcggctgcg cctggcctgc gagatcgccg gcatcgacgt gcgcaccttg
      601 cagcgctgga aggccgacga cggcctggtc gctggcgatg gacgccccga ggcggcgcgg
      661 cctacgcctg cgcacgcgct cagtcccgcc gagcgcgagt acatcgtgca cgtggccaac
      721 cagccgcgct ttgccgacat gccgcccgcg cgcatcgtgc ccgcgctggc cgacgagggc
      781 gtgtacgtag ccagcgaatc gagcttccag cgcgtgctgc gcgcgcacgg gcagacgcgt
      841 catcgtggtc gcgccaaggc gccgcgcaag agccgcccgc ccaccaccca cgtcgccacc
      901 gcgccgcgcc agctgtggtg ctgggacatg acgtatctgc ccgccgaggt catcggccgc
      961 tggttctacc tgtacctgat cctggacgtg tacagccgca agatcgtcgg cttcgaggtc
     1021 catgacagcg acgacggcga ccacgctgcg cacctgctca agcgcaccgc gctggccgag
     1081 ggcatccacg cgatgctcaa caagcccgtg ctgcacggcg acaacggctc cacgctcaag
     1141 gccaccagcg tgctggcgat gctgcactgg ctgggcgtca agccctcgta ctcgcgcccg
     1201 cgcgtgagcg acgacaacgc ctacgtcgag agcctgttcc gtacggcgaa gtaccgcccg
     1261 gagttccccg ccaagggctt tgccgacctc gaccaggcgc gggcgtgggc cgcccagttc
     1321 gtgcactggt acaaccacga tcatcgccac agcggcatcc gctacgtcag ccccgcacag
     1381 cgccacgccg gcgccgacac ggcgatcctc gcggcgcgcc acgagctgta ctgccgagcg
     1441 cgcgagcgca atccgcggcg ctggtccggg cccactcgga actggacgcc cgtcactgtg
     1501 gtgaccttga acccggagcg cgattcggtc gtcgagaccc attcgcgcac cctcgataa
//
LOCUS       DAOQNN010000647         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_76645_length_1554_cov_5.188125, whole genome shotgun sequence.
ACCESSION   DAOQNN010000647 DAOQNN010000000
VERSION     DAOQNN010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76645_length_1554_cov_5.188125"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PKV97_18250"
     CDS             <1..432
                     /locus_tag="PKV97_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNB07883.1"
                     /translation="NQEGRTVPASLPRVDFFHWVVVDLPAELRAIDAGHFSAEVTPGG
                     KPGPEAALGARHGVNDYTAWFAGDESMKGDYHGYDGPCPPWNDEIVHHYVFTLYALDV
                     ARCAVEGRFTGPDVRAAIAGHVLAEAKLTGTYSLNPALAAA"
     gene            503..1432
                     /locus_tag="PKV97_18255"
     CDS             503..1432
                     /locus_tag="PKV97_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HNB07884.1"
                     /translation="MFEFLQGIAFGLLVSFVPWFMAGMYDPRLVVPEELASRWQVILR
                     YAVAMPSIATLLLLTSLWGGFGASLGGWLAGLAAAPAALFLERRWRRWRQARAGKERA
                     AARERARTELARERRERDLLTLDPDFVPADADAVVMQLWEVKRELVGLRRPDLAVQAD
                     RLFTRYHRALDVLDEKFDRREVTYERARGLVGEVGSGAAAGLGAMVALVQGSVGLDAD
                     FVRERLEREAARIGGTEREALQRRLALADETERRLGEIAAGNEAALTALDHVAVAVSR
                     IDTGRGQPAGATEQALQELRRFAERAGLYGRQQ"
BASE COUNT          196 a          572 c          558 g          228 t
ORIGIN      
        1 aaccaggaag gccgcacggt gcccgccagc ctgccgcggg tggatttctt ccactgggtg
       61 gtggtcgacc tccccgccga actgcgcgcg atcgacgccg ggcatttctc cgccgaggtc
      121 accccgggcg gcaagcctgg gccggaggcc gcgctcggtg cgcgccacgg cgtgaacgat
      181 tacaccgcct ggtttgccgg cgacgaatcc atgaagggcg actatcacgg ctacgacggt
      241 ccctgcccgc cgtggaacga cgagatcgtg caccactacg tgttcacgct gtacgcgctc
      301 gacgtggcgc gctgcgcggt cgagggccgc ttcaccggcc cggacgtgcg cgcggcgatc
      361 gccggccacg tgctcgccga ggccaagctc accggcacct attcgctcaa ccctgcgctc
      421 gccgcggcgt aagggaaata ggggacagac cccggaaatt ggggtctgtc ccctattttc
      481 gcgctgaccc ggagtcgtct ggatgtttga attcctgcag gggatcgcct ttgggctgct
      541 ggtctccttc gtgccctggt tcatggccgg gatgtacgac ccgcgcctgg tggtgccgga
      601 ggagctcgcc tcgcgctggc aggtcatcct gcgctacgcg gtggccatgc cctcgatcgc
      661 gaccctgctg ctgctgacct cgctgtgggg cggcttcggc gccagcctgg gcggctggct
      721 cgccggcctc gccgcggcac cggccgcgct cttcctggag cggcgctggc ggcgctggcg
      781 gcaggcgcgt gccggcaagg agcgcgccgc cgcgcgcgaa cgtgcgcgca ccgagctcgc
      841 gcgtgaacgc cgcgagcgcg acctgctcac gctggacccc gatttcgttc ctgccgacgc
      901 cgatgcggtg gtgatgcagc tatgggaggt caagcgcgaa ctcgtcggcc tgcgtcggcc
      961 cgatctcgcc gtgcaggccg atcgcctgtt cacccgctac caccgcgcgc tcgacgtgct
     1021 cgacgagaag ttcgaccgcc gcgaagtcac ctacgagcgc gcccgcggcc tggtgggcga
     1081 ggtcggcagc ggcgctgccg ccgggctggg agcgatggtc gcgctggtgc agggcagtgt
     1141 cgggctggac gccgacttcg tgcgcgagcg cctggagcgc gaggcggccc gcatcggcgg
     1201 caccgagcgc gaggcgctgc agcgccggct cgcgcttgcc gacgagaccg aacgccgcct
     1261 gggcgagatc gccgccggca acgaggcggc gctgaccgcg ctcgaccacg tcgcggtggc
     1321 ggtgtcgcgc atcgacaccg gccgcggcca gcccgccggc gccaccgaac aggccctgca
     1381 ggagctgcgc cgcttcgccg agcgcgccgg cctctacggc cgccagcaat gaaccgagga
     1441 acctcccgat gagcctgccg ctgcgatccc ttctccgtat cctcccgcac gcccccgagg
     1501 gcgacccggg cggcggcggg cgcccgcgct cgggcctgtc gctgccgccg atcg
//
LOCUS       DAOQNN010000648         1548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_77225_length_1548_cov_8.048225, whole genome shotgun sequence.
ACCESSION   DAOQNN010000648 DAOQNN010000000
VERSION     DAOQNN010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77225_length_1548_cov_8.048225"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1548)
                     /locus_tag="PKV97_18260"
     CDS             complement(<1..>1548)
                     /locus_tag="PKV97_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HNB07885.1"
                     /translation="HETTKVELLTYARENGVKVVKPFMLVIARDTTHASQLKALIEST
                     AFYEGRYANKVIQVDSSRSGAEEEEMITKLLAVESVDEPTEIVIHVNMLKEGWDVTNL
                     YTIVPLRAANARTLIEQSIGRGLRLPYGKRTGVAAVDRLNIVAHDRFQEIIDEANRGD
                     SPIRLQKVILEAPSADDKKVSVQVGSGAMTRLGLTAAPTLAPAPTQAIAPSSPDPGAA
                     ATPPTPQTPPPVFTTESERQAALVVMEVIGKYEIKRDLVPTSQALLTPAVQSAILAEV
                     SERLKPSQGDLLAGTDGAAAPLDLSAVVSKTTAMLVQQTIDIPRISVVPTGEVTTGYH
                     PFKLGTLPNFQPGQREIVGQELRTNQQFTMSRESGIREKRFEDYIVKKLIDFDDIDYF
                     TQADLLYDLAGQAVAHYQAQNYAEAELHEIFDTYGAELARLIRAEMLAHFWEEASGYE
                     VQVSRGFTELKACNFTVAAGQPAQNYRTTVTEPGRIKQMLFGGFERCLYPLQKFDSDT
                     ERRFAVL"
BASE COUNT          225 a          480 c          524 g          319 t
ORIGIN      
        1 ggaggactgc aaagcgccgc tcggtgtccg agtcgaactt ctgcagcggg tagagacagc
       61 gctcgaagcc gccgaagagc atctgcttga tcctgcccgg ctcggtgacg gtggtgcggt
      121 agttctgggc cggctgacct gccgccacag tgaaattgca ggccttcagt tccgtgaagc
      181 cccgactgac ctgcacctcg tagccgctgg cttcttccca gaagtgggcc agcatttcgg
      241 cccgaatgag ccgggccagc tcggcaccat aggtgtcgaa aatctcgtgc agctcggctt
      301 cggcgtagtt ttgcgcctgg tagtgggcca ccgcctggcc ggccaggtcg tacagcagat
      361 cggcctgggt gaagtaatcg atgtcgtcga aatcgatcag tttcttgacg atgtaatctt
      421 cgaagcgctt ttcccggatg ccggactccc ggctcatcgt gaactgctga tttgtgcgca
      481 gctcctgccc cacgatttca cgctggccgg gctggaaatt gggcagcgta ccgagcttga
      541 acgggtggta cccagtcgtg acctcgccgg tgggcacgac ggagatccgt ggaatgtcga
      601 tggtctgctg aaccagcatc gccgtggtct tgctcaccac cgcggacaga tccagcgggg
      661 cggcggcccc gtctgtgcct gccagcaggt ccccctggga aggtttcagc cgttcgctca
      721 cttccgccag gatggccgat tgcacggcgg gtgtgagcag cgcctggctg gtgggcacca
      781 ggtcgcgctt gatttcgtac ttgccgatca cctccatcac caccagcgcc gcttgtcgtt
      841 cgctttcggt ggtgaagaca gggggcggcg tctggggcgt cggtggtgtc gctgctgcgc
      901 cggggtcggg ggagctcggc gcaatggcct gcgtcggcgc tggtgcaagg gtcggtgccg
      961 cggtgaggcc caggcgtgtc atggcgccag agccgacctg gacgctgacc ttcttgtcgt
     1021 cggcactcgg ggcttccagg atcaccttct gcaggcggat gggggaatcg ccccggttgg
     1081 cctcgtcgat gatttcctgg aagcggtcgt gcgccacgat gttcaggcgg tccaccgccg
     1141 ccacgccggt gcgcttgccg tagggcagcc gcaggccgcg gccgatcgac tgctcgatca
     1201 gcgtgcgcgc gttggccgcc cgcagcggca cgatggtgta gaggttggtg acgtcccagc
     1261 cctccttgag catgttcacg tggatgacga tctcggtggg ctcgtccacg ctctccacgg
     1321 ccagcagctt ggtgatcatc tcttcctcct ccgcaccgct gcggctggag tccacctgga
     1381 tcaccttgtt cgcgtagcgc ccctcgtaga aggccgtcga ttcgatcagc gccttgagct
     1441 gcgaggcgtg ggtggtgtcc cgcgcgatca cgagcatgaa gggcttcacc actttgacgc
     1501 cgttctcgcg ggcgtaggtg agcagctcga ccttggtggt ctcgtgca
//
LOCUS       DAOQNN010000649         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_77316_length_1547_cov_13.266756, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000649 DAOQNN010000000
VERSION     DAOQNN010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77316_length_1547_cov_13.266756"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1547
                     /locus_tag="PKV97_18265"
     CDS             <1..>1547
                     /locus_tag="PKV97_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HNB07886.1"
                     /translation="SVDEPTEIVIHVNMLKEGWDVTNLYTIVPLRAANARTLIEQSIG
                     RGLRLPYGKRTGVAAVDRLNIVAHDKFQEIIDEANRGDSPIRLKQVILDAPSADDRKV
                     SVQVQPVAAAWLGLADAPALTPGPSATGSGALNAPVFTTEAEKQAARVVMDVIGQYEV
                     RRDLAPTRSALLKPEVQKEILAEVAERLKPLQGELLSGADESVPALNLPDVVAKTTEI
                     VVQQTIDIPRIAVVPTGEVTTGFRPFTLDVSQLHLQPGEREIVGQMLRTNEQFTLAAE
                     IGLKEQRPEDYIVHALVDFDDVDYFTHADLLYDLAGQMVRHLQSYLSEAEAVSVLDRD
                     RRLIAREIHAQMMAHFFEEATEFEVQVSRGFTELKPCNYTATAGQAPRHVRDTVDAVG
                     AIRQMLFGGFTKCLYPLQKFDSDTERRFAVILERDASKWFKPAKGQFQIYYKLGTEQP
                     EYIPDFVAETDSMILMVETKARSDIETPEVQAKAAAAARWCQHASAYAASVGGKPWKY
                     LLVPHDEV"
BASE COUNT          300 a          500 c          513 g          234 t
ORIGIN      
        1 agcgtggacg agccgaccga gatcgtgatt cacgtcaaca tgctcaagga gggctgggac
       61 gtgaccaacc tctacaccat cgtgccgctg cgcgcggcca atgcccgcac gctgatcgag
      121 cagtccatcg ggcgcggcct gcgcctgccc tacggcaagc gcaccggcgt ggcggcggtg
      181 gatcggctga atatcgtcgc ccacgacaag tttcaggaaa tcatcgacga ggccaaccgt
      241 ggcgattcgc cgatccgtct gaagcaggtc atcctcgatg cgccgagcgc cgacgacagg
      301 aaggtcagcg tgcaggtgca gcccgttgcc gcggcgtggc tgggattggc ggatgctccg
      361 gccctcaccc ccggcccctc tgccacaggg agcggggcgc tgaatgcgcc ggtgttcacc
      421 accgaggcgg aaaagcaggc ggcgcgtgtg gtcatggacg tgatcggcca gtacgaggtc
      481 aggcgcgatc tggcgcccac ccgcagcgcg ctgctgaaac cagaagtgca gaaggaaatc
      541 ctcgccgaag tggcggagcg actgaagccg ctgcaaggcg aattgctgtc gggcgcggac
      601 gaatccgtgc ctgcactcaa cctgcccgac gtggtggcga agaccaccga gatcgtggtg
      661 cagcagacca tcgacatccc gcgcattgcg gtggtgccga ccggcgaggt cacgaccggc
      721 tttcgcccat tcacgctgga cgtgagccag ctccatcttc agccgggcga gcgcgagatc
      781 gtcgggcaga tgctgcggac caacgagcag ttcacgctgg cggcggaaat cggcctgaag
      841 gaacagcgcc cggaggacta catcgtccac gcgctggtgg acttcgacga cgttgactat
      901 ttcacccacg ccgacctgct ctacgacctc gcggggcaga tggtgcgaca cctgcaaagc
      961 tacctctcgg aagccgaggc ggtcagcgtg ctggatcggg atcgccgctt gatcgcgcgc
     1021 gaaatccacg cccagatgat ggcgcacttc ttcgaggagg cgaccgagtt cgaggtgcag
     1081 gtcagtcgcg gcttcaccga actcaagccg tgcaactaca cggcgacggc ggggcaagcc
     1141 ccgcgccacg tccgcgacac ggtggatgcc gtgggcgcca tccgccagat gctgttcggc
     1201 gggttcacga agtgcctgta ccccttgcag aagttcgact cggacaccga gcggcgcttc
     1261 gccgtcatcc tcgaacgcga cgccagcaag tggttcaagc ccgcgaaggg gcagttccag
     1321 atctactaca agctcggcac cgagcagccg gaatacatcc ccgacttcgt ggccgaaacc
     1381 gattcgatga ttctgatggt ggagaccaag gcgcgatcag acatcgagac gccagaggtg
     1441 caggccaagg ctgccgccgc cgcacgctgg tgccagcacg cctccgctta cgcggcgagc
     1501 gtcggcggca aaccgtggaa gtatctgctg gtgccgcacg acgaggt
//
LOCUS       DAOQNN010000650         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_77318_length_1547_cov_10.581769, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000650 DAOQNN010000000
VERSION     DAOQNN010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77318_length_1547_cov_10.581769"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..473)
                     /locus_tag="PKV97_18270"
     CDS             complement(3..473)
                     /locus_tag="PKV97_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNB07887.1"
                     /translation="MQKSFLAAPLLAALALFGSAVAHAEVTVAEPWVRATVPAQKATG
                     AFMRLKSDTDARLVSAASPVAGVVEIHEMLMDKDVMKMNRIPGLDLPAGKDVELEPGG
                     YHVMLMDLKAQVKEGDQVPVTLTVENKDGSRQTIELTAPARPLNAPMKMQHGKH"
     gene            complement(666..1082)
                     /locus_tag="PKV97_18275"
     CDS             complement(666..1082)
                     /locus_tag="PKV97_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
                     /protein_id="HNB07888.1"
                     /translation="MQRFRHRLHVWIAIFAMALSALAPAASRAMGPDDNGRYLVQICS
                     AVGMEWVELSVDEAAFHGDVGAMNADSDSHPSSLPLEECPYCLAQFGNVLAPAADLSP
                     SFAVAGGTALPTLFLVAPRPLFAWSSAHPRAPPARA"
     gene            complement(1159..>1547)
                     /locus_tag="PKV97_18280"
     CDS             complement(1159..>1547)
                     /locus_tag="PKV97_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB07889.1"
                     /translation="GSSVSEVLQRLRQLGVALSIDDFGTGFSGMSRLKRLAVQEVKID
                     RSFVIDLLASARDREIAAAIIDLSHRLGIEVTAEGVEDGRTAELLAGMGCDYIQGFLH
                     APALPLTDFVAWVRARECIGRQPVQT"
BASE COUNT          270 a          484 c          568 g          225 t
ORIGIN      
        1 gctcagtgct tgccgtgctg catcttcatc ggcgcattga gcggacgtgc cggcgcggtg
       61 agctcgatgg tctggcggct gccatccttg ttctcgacgg tcagcgtcac cggcacctgg
      121 tcaccctcct tcacctgagc cttcaaatcc atcagcatca cgtggtaacc gccaggctcg
      181 agttcgacgt ccttgccggc agggaggtcg agcccgggga tgcggttcat cttcatgacg
      241 tccttgtcca tgagcatctc gtggatctcg accacgccag cgaccggcga ggcggcggac
      301 acgaggcggg cgtcggtgtc ggacttcagg cgcatgaagg ccccggtggc cttctgcgcg
      361 ggcacggtgg cgcgcaccca aggctcggcg acggtgactt cggcgtgagc gacggcgctg
      421 ccgaaaaggg cgagggcggc cagcagcggt gcggccagga acgacttttg catcgtggat
      481 ctccgatcgt tggaggattg cggtccgcac gcgacgtgcc ggcgcgctcc ggcaaaggca
      541 gcgaagaacg cgggtttgcg tgcgaagggc tcgcacgacc ggcaggccgg cgggcgagga
      601 agggacttcg ggcgggggaa aggaccgcta cggcgaacac gcgcggcgtt cgcgcgtgcg
      661 cagggtcagg cgcgcgcggg cggcgcgcgc ggatgcgcgg acgaccacgc gaagagcggt
      721 cgcggggcga cgaggaaaag cgtggggagt gccgtcccgc cggcgacggc gaaggaaggc
      781 gagaggtcgg ccgcgggcgc cagcacgttg ccgaactgcg cgagacagta aggacactcc
      841 tcgagcggga gggaggacgg gtgcgaatcg gagtcggcgt tcatcgcacc gacgtcacca
      901 tggaaggcgg cctcgtcgac actcagttcc acccactcca tcccgacggc agagcaaatc
      961 tgcacgaggt agcggccgtt gtcgtcgggc cccatcgcgc ggctcgctgc cggcgccagc
     1021 gcactcaacg ccatcgcgaa gatcgcgatc cagacgtgaa gacgatgacg aaagcgctgc
     1081 atggggggat cggatccgtg gaagccacaa caacacggcg gattctaccg gatcggtccg
     1141 tgcttgaatt gatcgtggtc aggtttgcac cggctgccgg ccgatgcatt cgcgagcgcg
     1201 cacccaggcg acgaaatccg tcagcggcag ggccggggca tgcaggaaac cctggatgta
     1261 gtcgcagccc atgccggcga gcagttcggc ggtgcgaccg tcctccacgc cctcggcagt
     1321 gacctcgatg ccgagacggt gcgagaggtc gatgatcgcg gcggcgatct cgcggtcgcg
     1381 cgccgaggcg agcaggtcga tgacgaagct gcgatcgatc ttgacctcct gcacggcgag
     1441 ccgcttgagc cggctcatgc ccgagaagcc ggtgccgaag tcgtcgatcg acagggcgac
     1501 accgagctgg cgcaagcgtt ggagcacctc cgagacgctg ctgcccg
//
LOCUS       DAOQNN010000651         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_77609_length_1544_cov_6.863667, whole genome shotgun sequence.
ACCESSION   DAOQNN010000651 DAOQNN010000000
VERSION     DAOQNN010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77609_length_1544_cov_6.863667"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PKV97_18285"
     CDS             <1..996
                     /locus_tag="PKV97_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB07890.1"
                     /translation="PDVVRVVTDGRIPLLRDDLPMNGDPSQSLHTACDLVPSKPLTDV
                     VLVGRAPQLLPREDADVGAFARAALEADGVEVLTGVAALRCEQETAAGGTDKFLVVAD
                     PASGGERRIAFDTLLCAVGRSARLEGFGLEALGIPTGATVATDDFLQTRYPNILAAGD
                     VAGPLQFTHVAAHQAWHAAVNALFGHLRRFRVDYRCVPHTTFTDPEVARVGLNETEAH
                     ARGIAFEFTRYDLAELDRAITDGADRGFVKVLTVPGKDRILGATIVGEHAGELLAEFV
                     LAMKHGLGLNKLLGTIHAYPTFAEANKSVAGEWKRAHAPQRVLRWLERHHAWRRG"
     gene            complement(1010..>1544)
                     /locus_tag="PKV97_18290"
     CDS             complement(1010..>1544)
                     /locus_tag="PKV97_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB07891.1"
                     /translation="FSMYSSQWLAVIGFLPSIYAQAGLSGQVAGLLTAFACLANVTGN
                     VAAGRLLHRGVCARHLLYAGFLAMAATTFVAFSPLAAELPVARYLAVVLFSAIGGLIP
                     ATLFALAVHVAPDDDTVSTTVGWMQQCSSAGQFLGPPLVAWVAGAVGGWQWTWVITGL
                     ASLVGLVLSSRTRYQRA"
BASE COUNT          246 a          573 c          540 g          185 t
ORIGIN      
        1 cccgacgtgg tgcgagtcgt caccgacggg cggatcccgc tgctgcggga cgatctcccc
       61 atgaacgggg atccgtcgca gagcctgcac acggcctgcg atctggtgcc gtcgaagccg
      121 ctcacggacg tggtgctggt ggggcgcgcg ccgcagctcc tgccgcgcga ggacgccgat
      181 gtcggcgcct tcgcccgcgc cgcgctggag gcggacggtg tggaggtgct gaccggagtc
      241 gcggcattgc gctgcgagca ggaaacggca gcgggcggca cagacaaatt ccttgtcgtc
      301 gccgaccccg ccagcggcgg cgagcgccgc atcgccttcg acaccctgct gtgcgcggtc
      361 ggccgcagcg cccgcctgga gggcttcggc ctggaggcgc tcggcatccc gaccggcgcg
      421 acggtggcga ccgacgactt cctgcagacg cgctacccca acatcctcgc cgccggcgac
      481 gtcgccggcc ccctgcaatt cacccacgtc gccgcgcacc aggcctggca cgcggcggtc
      541 aatgcgctct tcggccacct gcgccgtttc cgcgtcgact accgctgcgt gccccacacc
      601 accttcaccg accccgaggt cgcccgcgtc gggctcaacg agaccgaggc gcacgcacgc
      661 ggcatcgcgt tcgagttcac ccgctacgat ctggccgagc tcgaccgcgc aatcaccgat
      721 ggcgccgacc gcggcttcgt caaggtgctg accgtgcccg gcaaggaccg catcctcggc
      781 gccaccatcg tcggcgagca cgccggcgag ttgctcgccg agttcgtgct ggcgatgaag
      841 cacggtctgg gcctgaacaa gctcctcggc acgatccacg cctacccgac cttcgccgag
      901 gccaacaagt cggtcgccgg cgaatggaag cgcgcgcacg cgccgcagcg cgtgctgcgc
      961 tggctggaac gccaccacgc ctggcggcgg ggctgacgcg ccgcgcgcat caggcgcgct
     1021 ggtagcgcgt ccggctcgac agcaccaggc cgacaaggga ggcgagcccg gtgatcaccc
     1081 aggtccattg ccagccgccg acagcgcccg ccacccaggc caccagcggc gggccgagga
     1141 actggccggc cgaggagcac tgctgcatcc agcccacggt ggtcgacacc gtgtcgtcgt
     1201 cgggcgccac atgcaccgcg agcgcgaaca gggtcgccgg gataagcccg ccgatcgccg
     1261 agaacagcac cacggccagg taacgcgcga ccggcaactc tgccgcgagc ggcgagaagg
     1321 cgacgaaggt ggtcgccgcc atggcgagga agccggcgta cagcaggtgg cgcgcgcaca
     1381 cgccgcgatg cagcaggcgg ccggcggcga cgttgccggt gacgttcgcg aggcaggcga
     1441 aggcggtcag cagcccggcc acctggcccg acagtccggc ctgggcgtag atcgacggca
     1501 ggaatccgat caccgccagc cactggctgg agtacatgga gaag
//
LOCUS       DAOQNN010000652         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_77959_length_1540_cov_6.500337, whole genome shotgun sequence.
ACCESSION   DAOQNN010000652 DAOQNN010000000
VERSION     DAOQNN010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77959_length_1540_cov_6.500337"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1540
                     /locus_tag="PKV97_18295"
     CDS             <1..>1540
                     /locus_tag="PKV97_18295"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNB07892.1"
                     /translation="DPGRILLLTFSRRAADEMGRRVRRILAQAAADRPGLARAELQWA
                     GTFHAIGARLLRDHAGRIGLDPGFSIHDREDSADLMNLVRHELGLSATKQRFPQKGTC
                     LAIYSAVVNTRAALAEVLQASFPWCAQWEDELKCLFRAYVEAKQAQQVLDYDDLLLYW
                     AQMVGEAELGAEIGGLFDHVLVDEYQDTNALQAAILLAMKPDGRGLTVVGDDAQSIYA
                     FRGATVRNILDFPAAFAPPARVVTLERNYRSTQPILDAANAVIALAAERFAKNLWSER
                     ASAQRPRLVTVKDEAAQADFVVDSVLARREEGLKLKQQAVLFRASAHSARLELELTRR
                     NIPFVKYGGLRFLEAAHVKDVLALLRWAENPRDRIAGFRAIQLLAGIGPRTAGRVLDN
                     VCSAPPGCVLLAEQPVPEAARPGWLEFATLVDRLSGREEASTAAFAPGGTPARTTAPV
                     HAEGLAGGARGGLPWPTDFELACRWYEAQMPRLYDDFQVRVLDIQQLARIAATSPSRQ
                     RFLT"
BASE COUNT          213 a          571 c          544 g          212 t
ORIGIN      
        1 ccgaccccgg acgcatcctg ctgctgacct tctcgcgccg cgccgcggac gagatgggcc
       61 ggcgcgtgcg tcgcatcctc gcgcaggccg ccgccgaccg tcccggcctg gcgcgcgccg
      121 agctgcagtg ggcgggcacc ttccacgcca tcggcgcgcg cctgctgcgc gaccacgccg
      181 gccgcatcgg gctcgacccc ggcttcagca tccacgaccg cgaggactcc gccgacctga
      241 tgaacctggt gcgccacgag ctcgggctgt cggcgacgaa gcagcgcttc ccgcagaagg
      301 gcacctgcct ggcgatctat tcggcggtgg tcaatacgcg cgccgcgctc gccgaggtgc
      361 tgcaggcgag cttcccgtgg tgcgcgcagt gggaggacga gctcaagtgc ctgttccgcg
      421 cctacgtcga ggccaagcag gcgcagcagg tgctcgacta cgacgacctg ctgctgtact
      481 gggcgcagat ggtgggcgag gcggagctcg gtgccgaaat cggcggcctc ttcgaccacg
      541 tgctggtcga cgaataccag gacaccaacg cgctgcaggc ggcgatcctg ctcgcgatga
      601 agccggacgg ccgcggcctg accgtggtcg gcgacgacgc gcagtcgatc tacgccttcc
      661 gcggcgccac ggtgcgcaac atcctcgact tcccggcggc cttcgcgccg ccggcgcggg
      721 tggtgacgct ggagcgcaac taccgctcca cccagcccat cctcgacgcc gccaacgcgg
      781 tgatcgcgct cgccgccgag cgcttcgcca agaacctgtg gagcgagcgc gcctcggcgc
      841 agcgcccgcg cctggtgacg gtgaaggacg aggcggcgca ggccgacttc gtggtcgaca
      901 gcgtgctggc gcgccgcgag gaagggctga agctcaagca gcaggcggtg ctgttccgcg
      961 cctcggcgca cagcgcacgc ctggaactgg agctgacccg gcgcaacatc cccttcgtga
     1021 agtacggcgg gctcaggttc ctcgaggccg cccacgtcaa ggacgtgctc gcgctcctgc
     1081 gctgggcgga gaacccgcgc gaccgcatcg ccggcttccg tgccatccag ctgctcgccg
     1141 gcatcggccc caggaccgcc gggcgcgtgc tcgacaacgt ctgcagcgcg ccgccgggct
     1201 gcgtgctgct cgccgagcag ccggtgccgg aggcggcgcg cccggggtgg ctggagttcg
     1261 ccacgctggt cgaccggctt tccgggcggg aggaagcctc gaccgccgca ttcgcgcctg
     1321 gcggcactcc tgcacgaacc accgcgcctg tccatgcgga gggtcttgcg ggcggggcga
     1381 ggggcggcct gccgtggccg accgacttcg agctcgcctg ccgttggtac gaggcgcaga
     1441 tgccgcgcct gtacgacgat ttccaggtgc gcgtgctcga catccagcaa ctcgcgcgca
     1501 tcgccgccac cagcccgagc cggcagcgct ttctcaccga
//
LOCUS       DAOQNN010000653         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_78421_length_1535_cov_11.990541, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000653 DAOQNN010000000
VERSION     DAOQNN010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78421_length_1535_cov_11.990541"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..391)
                     /locus_tag="PKV97_18300"
     CDS             complement(98..391)
                     /locus_tag="PKV97_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07893.1"
                     /translation="MQFSMLNFFTGRQHQLGALVLMRGETFIETIEEGGMAVLVLLRG
                     TASVSRQRAGAQVGLHLTPRLGEPVWLTSPGTYCVVAKDHVIGMRMLKIGGGT"
     gene            complement(395..598)
                     /locus_tag="PKV97_18305"
     CDS             complement(395..598)
                     /locus_tag="PKV97_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07894.1"
                     /translation="MSAAIEEVVIEVLPREPSEEAKPPRVELLDGRLCTEVELKAAVI
                     ASGIVCALAGFGLGYLMGREGRR"
     gene            complement(601..1278)
                     /locus_tag="PKV97_18310"
     CDS             complement(601..1278)
                     /locus_tag="PKV97_18310"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07895.1"
                     /translation="MSRGVIVTALVTGTALVGAGYYLTRQQRPRALPTSVVSIITPVI
                     DLGVAEEVLAAMQRIPGDEITVVLHTAGGCVTSCVLIANALREFPRSTAIVPYMAISG
                     GTLIALNARALQMGRSASLSAVDPIVFGQRARHISPQEQAPGVHALAKEYERAMSRYL
                     RETLAARLPDASDAQLERALGVFLGEDAPHEWPIRRHQVEALGLPVSPATRVWSDMVD
                     GYRGRWW"
     gene            complement(1275..>1535)
                     /locus_tag="PKV97_18315"
     CDS             complement(1275..>1535)
                     /locus_tag="PKV97_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07896.1"
                     /translation="LTRDYWRAIENITHAVSMTTQAGASPTRAHAAVRDYLLGRLALE
                     QSLIHTLLELPREQHVDTHAHRAAPTTRARTSRSTRHGNGHA"
BASE COUNT          211 a          523 c          572 g          229 t
ORIGIN      
        1 gcctccgcgt gcttgctgcg gatcgcctgc cagatcatcc ggcggtagtg gcggtagctc
       61 gccgtctccg gcgcgctctc gaggtcgtcc aggtccgtca tgtcccgcct ccgatcttga
      121 gcatgcgcat gccgatcacg tggtccttgg cgacgacgca gtaggtcccc gggctcgtca
      181 gccacaccgg ctcgccgagg cgcggcgtga gatggaggcc gacctgcgcg ccggcccgct
      241 ggcgcgagac cgaggcggtg ccgcgcagca gcacgagcac ggccatgccg ccctcctcga
      301 tcgtctcgat gaaggtctcg ccgcgcatca gcaccagtgc gccgagctgg tgctggcgtc
      361 cggtgaagaa gttgagcatc gagaattgca cgggctacct cctcccctcg cggcccatca
      421 ggtatccgag gccgaagccg gcgagcgcgc agacgatgcc cgaggcgatc acggcggcct
      481 tgagctcgac ctcggtgcag agccggccgt ccaggagctc gacgcgcggg ggctttgcct
      541 cctcggacgg ctccctcggc aggacctcga tcacgacctc ttcgatcgcg gcggacatcg
      601 tcaccaccac ctcccccggt acccatcgac catgtcggac cacacgcgcg tcgccggcga
      661 gaccggcagg ccgagcgcct cgacttgatg ccggcggatc ggccactcgt gcggggcgtc
      721 ttcgccgagg aagacgccga gcgcccgctc gagctgggcg tcgctcgcgt cgggcaggcg
      781 cgccgccagc gtctcgcgca agtagcggct catcgcgcgc tcgtactcct tggcgagcgc
      841 gtggaccccc ggcgcctgct cctgcggcga gatgtggcgg gcgcgttggc cgaagacgat
      901 cgggtcgacg gccgagagcg acgcgctgcg gcccatctgg agcgcgcgcg cgttgagggc
      961 gatcagcgtg ccgccggaga tcgccatgta ggggacgatc gcggtcgagc gcgggaactc
     1021 ccgcagggcg ttggcgatca gcacgcacga ggtcacgcag ccgccggccg tgtgcaggac
     1081 gaccgtgatc tcgtcgccgg ggatccgctg catcgccgcg agcacctctt cggcgacgcc
     1141 gaggtcgatc accggggtga tgatcgagac gacgctggtc ggcagcgccc gcgggcgttg
     1201 ctggcgggtc aggtagtagc cggcgccgac cagggcggtg ccggtgacca gcgccgtgac
     1261 gatgactccg cggctcatgc gtgcccgttt ccgtggcggg tggaacgaga ggtgcgcgcg
     1321 cgagtcgtcg gcgcagcgcg atgggcatgc gtgtcgacgt gttgctcgcg cgggagctcg
     1381 agcaaggtgt ggatgaggga ctgctcgagc gcgaggcggc cgagcaggta gtcgcggacg
     1441 gccgcgtggg cgcgcgtcgg cgatgctccg gcctgcgtcg tcatcgagac ggcgtgggtg
     1501 atgttctcga tcgcgcgcca atagtcgcgc gtgag
//
LOCUS       DAOQNN010000654         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_78425_length_1535_cov_7.429054, whole genome shotgun sequence.
ACCESSION   DAOQNN010000654 DAOQNN010000000
VERSION     DAOQNN010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78425_length_1535_cov_7.429054"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            524..1513
                     /locus_tag="PKV97_18320"
     CDS             524..1513
                     /locus_tag="PKV97_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB07897.1"
                     /translation="MLQLFARLIGIVLLALAAGGAALAADRPVVRVGVLQFGTVSWEL
                     ETMQRHGLLEREGVDIRIVPLALKDAANVALQGGEVDVIVNDWLWVARMRSEGADFVF
                     VPFSQAVGGIHARPDAGIAGFADLRGQRLGVAGGALDKSWLLLRAYARRTVGEDAASF
                     LRPQFAAPPLLNELVTRGELPAAMNFWHYGARLAAAGMPEVLGMKEILATLDIGDEMP
                     LVGWVFGERWAGANPAAIRGFLRASAAAKALLRESDAAWEALRPSMRVEDEATFVALR
                     DGFRAGIPQAADEEGERAAARAFAILAAEGGEALVGRARELAPGTFWHGGGGR"
BASE COUNT          174 a          519 c          619 g          223 t
ORIGIN      
        1 gcacggtgat cgtggggctg tcgagctggc ggtggagatc ctccatcgcc ggcgccgaca
       61 gcgcgagcag gcgctcttgg tcgagggcca cgtcggcgcc gcggcgatcg cgcgccgcgt
      121 cgtcgacggg gtgcccgacc gtgcgcgtcg tcggcgtgcc ccggggggga ggcggggcgg
      181 gggaagctgc gggagtcggt aggctggaca tcgctgtggt ggtcctggag tcgctgcggc
      241 cgaggccgcc cgtggccacc catgcaggat tcgtgccgag gatgacgagg gggtgcggac
      301 cgcggcggga cctgcttgca tgcagatgcc ggcgcatagt tgttgcgtcc ctgagcaagc
      361 ttgctcccaa acggaacagt cgccgctgcg ccagcgctgc tgcgccgcgg gcagtcgcgt
      421 gcaggggggc gcgatgtcga tcgctggcac ggaacctgct ggggaatcgg cacaaaaggc
      481 ctgcgcccgt tcccgggtca tgcaagacaa cggaggagac cccatgctgc agctgttcgc
      541 ccgcctgatc ggcatcgtcc tgctggcgct cgccgcgggg ggcgccgcgc tcgccgccga
      601 ccggccggtc gtccgtgtcg gcgtgctgca gttcggcacg gtgagctggg agctcgagac
      661 catgcagcgc cacggcctgc tcgagcgcga gggggtggac atccgcatcg tcccgctcgc
      721 gctcaaggac gccgccaacg tcgccctcca gggcggcgag gtcgatgtca tcgtcaatga
      781 ctggctgtgg gtggcgcgca tgcgctcgga gggggcggac ttcgtcttcg tgcccttctc
      841 gcaggcggtc ggcggcatcc atgcgcgccc ggacgcgggc atcgccggtt tcgccgacct
      901 gcgtggccag cgcctcggcg tggcgggcgg cgcgctcgac aagagctggc tgctgctgcg
      961 cgcgtatgcc cgccggactg tgggcgagga cgccgcgagc ttcctgcgcc cgcagttcgc
     1021 cgcgccgccg ctgctcaacg agctggtgac gcgcggcgag ctgccggcgg cgatgaactt
     1081 ctggcactac ggggcgcgcc tcgccgccgc cggtatgccc gaggtgctgg gcatgaagga
     1141 gatcctcgcc acgctcgata tcggcgacga gatgccgctg gtgggctggg tgttcggcga
     1201 gcgctgggcg ggggcgaatc cggccgcgat ccggggcttc ctgcgcgcct ccgcggcggc
     1261 caaggcgctg ctgcgcgagt ccgacgcggc ctgggaggcg ttgcgcccgt cgatgcgcgt
     1321 cgaggatgag gcgaccttcg tcgcgctgcg cgatgggttc cgcgccggca tcccgcaggc
     1381 tgccgacgag gagggcgagc gcgccgccgc gcgcgccttc gcgatcctcg cggccgaggg
     1441 cggcgaggcg ctggtcgggc gcgcccgcga gctcgcgccg ggcaccttct ggcatggggg
     1501 cggggggcgg tgagcgcgcg gcgcctcgcc ccgcc
//
LOCUS       DAOQNN010000655         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_78707_length_1532_cov_5.934326, whole genome shotgun sequence.
ACCESSION   DAOQNN010000655 DAOQNN010000000
VERSION     DAOQNN010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78707_length_1532_cov_5.934326"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKV97_18325"
     CDS             <1..322
                     /locus_tag="PKV97_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNB07898.1"
                     /translation="LLEGLEGEGAAPPLVLWALAHETRSLATLSAGRDAGQPLAALFK
                     AERIFDPRRQQAMTRALGRLGQGHLRAALMHAARIDRMIKGLASGDLWDEFLQLALRL
                     ARRG"
     gene            432..>1532
                     /locus_tag="PKV97_18330"
     CDS             432..>1532
                     /locus_tag="PKV97_18330"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNB07899.1"
                     /translation="MDIQHYMQTLGRQARAASRVLAAASTAAKNAALIAMAAEIRARR
                     AELLAANAQDLDEARAAGLEPALIDRLTLDEKGIESMAAGLEQVAALPDPVGEMSDIK
                     RRPSGIALGKMRVPLGVIGIIYEARPNVTADAAALCLKSGNAAILRGGKEAIHANRAI
                     AACVRAGLLAAGLPEHAVQVVEITDREAVGALITMPEFVDVIVPRGGKGLIERISRDA
                     RVPVIKHLDGNCHVYIDDEADPAKVVPIVENSKTQRYGTCNTAESLLVARDVADIYLP
                     EIARTLAAKGVEMRCCAESLALLREAGIAGAKLVEATAADWREEYLAPIIAVKIVAGL
                     DEAIEHINTCSSGHTEAIVTENYSRAMRFLREV"
BASE COUNT          238 a          590 c          504 g          200 t
ORIGIN      
        1 cctgctcgaa ggcctcgagg gcgagggcgc cgcccccccg ctggtgctgt gggcgctcgc
       61 gcacgagaca cgcagcctcg ccaccctgag cgcgggccgc gacgccggcc agcccctggc
      121 cgccctgttc aaggccgagc gcatcttcga tccgcgccgc cagcaggcca tgacccgtgc
      181 cctcggtcgc ctcggccagg gccatctgcg cgccgcgctg atgcacgcgg cgcgcatcga
      241 ccgcatgatc aagggcctcg ccagcggcga cctgtgggac gagttcctcc agctcgcgct
      301 gcgcctggcg cgccgcggct gaaccgcggc cgacccgcgc caggcttcat ccgcatccgg
      361 ttcgatgatt taatgcggat ttgccccgcg gcgtccgtgg atgccgcgac cgacgccgaa
      421 gaagagcccc catggacatc cagcactaca tgcagaccct gggccgccag gcccgcgccg
      481 cctcgcgcgt gctcgccgcc gcctccaccg cggccaagaa cgccgccctg atcgcgatgg
      541 ccgccgagat ccgcgcccgc cgcgccgaac tgctcgccgc caatgcccaa gacctcgacg
      601 aggcgcgtgc cgccggcctg gagccggcgc tgatcgaccg cctgacgctg gacgagaaag
      661 gcatcgagtc catggccgcc ggcctcgagc aggtcgccgc gctgcccgac ccggtgggcg
      721 agatgagcga catcaagcgc cgcccctccg gcatcgcgct cggcaagatg cgcgtgccgc
      781 tgggcgtgat cggcatcatc tacgaggccc gccccaacgt caccgccgac gccgcggcgc
      841 tgtgcctcaa atccggcaat gccgcgatcc tgcgcggcgg caaggaggcg atccacgcca
      901 accgcgcgat cgccgcctgc gtgcgcgccg gcctgctggc ggccggtctg cccgaacacg
      961 cggtgcaggt ggtcgagatc accgaccgcg aggccgtcgg tgcgctgatc accatgcccg
     1021 agttcgtcga cgtgatcgtg ccgcgcgggg gcaagggcct gatcgagcgc atctccaggg
     1081 acgcgcgcgt gccggtgatc aagcacctcg acggcaactg ccatgtttat atcgacgatg
     1141 aggccgaccc tgccaaggtc gtacccatcg tcgagaactc caagacccag cgctatggca
     1201 cctgcaacac cgccgaatcg ctgctggtgg cgcgcgacgt cgccgacatc tacctgcccg
     1261 agatcgcccg cacgctcgcc gccaagggcg tggagatgcg ctgctgcgcc gagtcgctcg
     1321 ccctcctgcg cgaggccggc atcgccgggg cgaagctggt ggaggcgacc gcggccgact
     1381 ggcgcgagga atacctcgcc ccgatcatcg cggtgaagat cgtcgccggc ctcgacgagg
     1441 cgatcgagca catcaacacc tgcagctcgg gccacaccga agcgatcgtc accgagaact
     1501 attcccgcgc catgcgcttc ctgcgcgagg ta
//
LOCUS       DAOQNN010000656         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_79527_length_1524_cov_6.904697, whole genome shotgun sequence.
ACCESSION   DAOQNN010000656 DAOQNN010000000
VERSION     DAOQNN010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79527_length_1524_cov_6.904697"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PKV97_18335"
     CDS             <1..742
                     /locus_tag="PKV97_18335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNB07900.1"
                     /translation="ARQLKVELEMVTPSGADGVITREAVQRAARVLAEAGPAEALRGV
                     RRAMAQNMSLAQSEVAGATVIDDAVIHAWPPGTDVTVRLIRALVAGCRAAPALNAWYE
                     SHAMARRLVPQVHVGIAVDLPDGLFVPVLRDVANRSPDDLRQGLERMRADVRARRIPP
                     EEMRGHTITLSNFGMIGGKYASPLVVPPTVAILGAGRVHEQVLAVDGAPAVRRVLPLS
                     LSFDHRVVTGGEAARFLKAVIDDLERAD"
     gene            complement(751..>1524)
                     /locus_tag="PKV97_18340"
     CDS             complement(751..>1524)
                     /locus_tag="PKV97_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB07901.1"
                     /translation="VEAGLWIALEQRQFVLHYQPQVAADGAVLGVEALVRWHHPVRGL
                     VPPGEFIPLAEECGLILPLGRWVLEAACAQLAAWADDPWRSRLVIAVNVSARQFLHED
                     FVAGVVAALECSGADPGRLDLELTESSLVTDVDKVSEKMEALKALGVGFSLDDFGTGY
                     SSLAYLKRLPLDQLKIDRSFVRDILVDPNDAAIARMIIVLADNLGLRVIAEGVECEEQ
                     RDFLARNGCSTYQGYLFSAPQPIDALEAFLDARRSRPAR"
BASE COUNT          230 a          561 c          529 g          204 t
ORIGIN      
        1 ggcgcgccag ttgaaggtcg agctcgagat ggtgacgccc tccggcgccg acggcgtgat
       61 cacccgcgag gccgtgcagc gcgccgcgcg cgtgctcgcc gaggccggcc ccgccgaagc
      121 gctgcgcggc gtgcgccggg cgatggcgca gaacatgagc ctggcgcaga gcgaggtcgc
      181 cggcgccacc gtgatcgacg acgcggtgat ccacgcctgg ccgcccggta ccgacgtcac
      241 cgtgcgcctg atccgcgccc tggtcgccgg ctgccgcgcc gcgccggcgc tcaacgcctg
      301 gtacgagagc cacgccatgg cgcgccggct ggtgccgcag gtgcacgtcg gcatcgcggt
      361 cgacctgccc gacggcctct tcgtgccggt gctgcgcgac gtcgccaacc gcagccccga
      421 cgacctgcgc cagggcctcg agcgcatgcg cgccgacgtc agggcgcgca ggattccgcc
      481 cgaggagatg cgcggccaca ccatcacgct gtccaacttc ggcatgatcg gcggcaagta
      541 cgcctcgccg ctggtggtgc cgcccacggt ggccatcctc ggcgccgggc gggtgcacga
      601 gcaggtgctc gcggtcgacg gcgccccggc ggtgcgccgc gtgctgccgc tgagcctgtc
      661 cttcgaccac cgcgtggtca ccggcggcga ggccgcgcgc ttcctgaagg cggtgatcga
      721 cgatctggaa cgcgccgact gagcgctcct tcagcgcgcc ggccgagacc ttcgggcgtc
      781 gaggaaggcc tcgagcgcgt cgatcggctg cggcgcactg aaaaggtagc cctgataggt
      841 cgagcagccg ttgcgggcga ggaagtcgcg ctgctcctcg cactccacgc cttccgcgat
      901 cacacgcagg ccgaggttgt cggcgagcac gatgatcatg cgcgcgatcg ccgcgtcgtt
      961 gggatcgacc aggatgtcgc gcacgaagct gcgatcgatc ttcaactgat ccagcggcaa
     1021 gcgcttgaga taggcgagcg aggaatagcc ggtcccgaag tcgtcgagcg agaatcccac
     1081 gccgagcgcc ttgagggcct ccatcttctc gctgaccttg tcgacgtcgg tcaccagcga
     1141 gctctcggtc agctccaggt cgagccggcc cgggtcggcc cccgagcact cgagcgcggc
     1201 gaccaccccg gcgacgaagt cttcatggag gaactggcgt gcgctgacat tgaccgcgat
     1261 caccagcctg ctgcgccacg ggtcgtccgc ccacgccgcc agctgcgcgc aggcggcctc
     1321 gagcacccag cgcccgagtg gaaggatcaa cccgcactct tcggcgagcg gaatgaactc
     1381 acccggcggt accaggccgc gcaccgggtg atgccaacgc accagcgcct ccacgccgag
     1441 caccgcaccg tcggcggcga cctggggctg gtaatgcagc acgaactggc gctgctcgag
     1501 cgcgatccac agccctgcct ccac
//
LOCUS       DAOQNN010000657         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_80629_length_1513_cov_8.570645, whole genome shotgun sequence.
ACCESSION   DAOQNN010000657 DAOQNN010000000
VERSION     DAOQNN010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80629_length_1513_cov_8.570645"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKV97_18345"
     CDS             <1..374
                     /locus_tag="PKV97_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNB07902.1"
                     /translation="KQFDVMVTGNMFGDILSDEASMLTGSIGMLPSASLNADNKGMYE
                     PSHGSAPDIAGKDLANPLATILSAAMMLRYTFNQEAAAQRIERAVKKVLAQGYRTGDI
                     YEPGTKRVGTREMGDAVLAAL"
     gene            429..>1513
                     /gene="asd"
                     /locus_tag="PKV97_18350"
     CDS             429..>1513
                     /gene="asd"
                     /locus_tag="PKV97_18350"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNB07903.1"
                     /translation="MNRVGLVGWRGMVGSVLMQRMVEEGDFAFIEPVYFSTSNAGGKA
                     PSFGGKEAAAPLQDASNIDALKSCDIVITCQGGDYTKEVFPKLRATGWNGHWIDAASA
                     LRMNDDAVIILDPVNRNVIDAALAKGGRNWIGGNCTVSLMLMGLGGLFRHGLVEWISA
                     MTYQAASGAGAQNMRELISQMGTIHDSVKDLLADPASAILEIDRKVAETMRSDAFPKK
                     NFRNTPLAGSLIPWIDVPVEHGQSKEEWKGGAECNKILGNPAFRTPGSIPIDGLCVRI
                     GAMRCHSQALTIKLKKDVPLDELTEIIAGANPWVKVVPNDREITERELTPTAVTGTLT
                     VPVGRLHKMAMGPEYLGAFTVGDQLLWG"
BASE COUNT          280 a          519 c          480 g          234 t
ORIGIN      
        1 ccaagcagtt cgacgtgatg gtcaccggca acatgttcgg cgacatcctg tccgacgagg
       61 cctcgatgct caccggctcg atcggcatgc tgccctcggc ctcgctcaat gccgacaaca
      121 agggcatgta cgagccctcg cacggctccg cgcccgacat cgccggcaag gatctcgcca
      181 acccgctcgc caccatcctg tcggcggcga tgatgctgcg ctataccttc aatcaggagg
      241 ccgctgccca gcgcatcgag cgcgcggtca agaaggtgct ggcccagggc tatcgtaccg
      301 gcgacatcta cgagccgggc acgaagcgcg tcggcacccg cgagatgggc gacgccgtgc
      361 tcgcagcgct gtaagctcgc gcgctggcaa ggctttttcg aagaacgttt ttgttgagag
      421 tgatgaacat gaatcgagtc ggtctggttg gctggcgtgg catggtcggt tccgtgctga
      481 tgcagcggat ggtggaggag ggcgactttg ccttcatcga gccggtgtat ttttccacct
      541 cgaacgccgg cggcaaggcg ccgtccttcg gcggcaagga agcggccgcg ccgctgcagg
      601 acgccagcaa catcgacgca ctgaagtcct gcgatatcgt gatcacctgc caaggcggcg
      661 actacaccaa ggaagtcttc cccaagctgc gcgccaccgg ctggaacggc cactggatcg
      721 acgccgcctc ggcgctgcgc atgaacgacg acgcggtgat catcctcgac ccggtcaacc
      781 gcaacgtcat cgacgccgcg ctcgccaagg gcgggcgcaa ctggatcggc ggcaactgca
      841 ccgtgtcgct gatgctgatg ggcctgggcg gcctgttcag gcacggcctg gtggagtgga
      901 tctcggcgat gacctaccag gcggcctcgg gcgccggcgc gcagaacatg cgcgaactca
      961 tcagccagat gggcaccatc cacgactcgg tcaaggacct gctcgccgac ccggcctcgg
     1021 ccatcctcga gatcgaccgc aaggttgccg aaaccatgcg ctcggacgcc ttcccgaaga
     1081 agaacttccg caacaccccg ctcgccggca gcctcatccc gtggatcgac gtcccggtcg
     1141 agcacggcca gagcaaggaa gaatggaagg gcggcgccga gtgcaacaag atcctcggca
     1201 accccgcctt ccgtacccca ggctcgatcc cgatcgacgg cctgtgcgtg cgcatcggcg
     1261 cgatgcgctg ccactcgcag gcgctcacca tcaagctcaa gaaggacgtg ccgctcgacg
     1321 agctcaccga gatcatcgcc ggcgccaacc cgtgggtgaa ggtggtgccc aacgaccgcg
     1381 agatcaccga gcgcgaactg actccgaccg ccgtcaccgg cacgctgacc gtcccggtcg
     1441 ggcgcctgca caagatggcg atgggtcccg agtacctcgg cgccttcacc gtcggcgacc
     1501 agctgctgtg ggg
//
LOCUS       DAOQNN010000658         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_80731_length_1512_cov_10.673988, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000658 DAOQNN010000000
VERSION     DAOQNN010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80731_length_1512_cov_10.673988"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKV97_18355"
     CDS             complement(<1..571)
                     /locus_tag="PKV97_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07904.1"
                     /translation="MAVSVETVINNVARTLLDTAFRTWSRAEHVANLNIAQRLICGDY
                     KLDAYPKREFVALVAGIAQDIPAEGTALIDITDNEVSQRSVTQTDLAILQEENRFWPR
                     GTQQTEVENYAADPRTPRKFYVFPPNNGSGSVRITYGAIPPETTGSSGESIALSDAYQ
                     YPIERIMLSLAFAKNSVRQDLSKSQAFMNE"
     gene            complement(580..960)
                     /locus_tag="PKV97_18360"
     CDS             complement(580..960)
                     /locus_tag="PKV97_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07905.1"
                     /translation="MITDAQEAALAARRAQTAQDKRNPFLIHVDDGRLMPNVARLRGH
                     AKYRVFTGSPKATPEERMAWLRSMGNGTPLPTEDPFDIGTASVAELIAFAASEYGVTL
                     DPSTHHNKMRAELRRLAAEAGNLA"
     gene            complement(1032..1232)
                     /locus_tag="PKV97_18365"
     CDS             complement(1032..1232)
                     /locus_tag="PKV97_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07906.1"
                     /translation="MKTSNPYGANYQVKVPPETIQKEQSQASGKAKARYPHTPLGPSQ
                     KNGEAGKMKRPAYTPGTSPAGS"
     gene            complement(1292..>1512)
                     /locus_tag="PKV97_18370"
     CDS             complement(1292..>1512)
                     /locus_tag="PKV97_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07907.1"
                     /translation="YQSRNLLTQTSPGPASYVMFGHSAGLTFASQIVECQMIDNPNDF
                     GYIIRGLMVFGYEVIGPKYVGTAVVAKG"
BASE COUNT          228 a          460 c          525 g          299 t
ORIGIN      
        1 cttcgttcat gaaggcctgc gacttgctca gatcctggcg caccgagttc ttggcaaaag
       61 ccagcgagag catgatgcgc tcgatcgggt actggtaggc gtcactcaat gcgatgctct
      121 ccccgctaga accggtggtc tcgggcggaa tcgcgccgta ggtgatgcgc acactacctg
      181 agccgttgtt cggcgggaag acgtagaact tgcgcggggt gcgcggatcg gcggcgtagt
      241 tctccacctc ggtctgctgt gtgccgcgcg gccagaagcg gttctcttcc tgcagaatcg
      301 caagatccgt ctgggtcaca ctgcgctgcg acacctcgtt gtcggtgatg tcgatcaatg
      361 cggtgccctc ggcagggatg tcctgcgcga tgccggccac cagtgcgacg aactcgcgct
      421 tggggtaggc gtcgagcttg tagtcgccgc agatcagacg ctgcgcgatg ttgaggttgg
      481 ccacgtgctc ggcacgcgac catgtacgga aagccgtgtc cagcagcgtg cgcgccacat
      541 tgttgatgac ggtctcgacg gacacggcca tgggtcacct catgccaggt tgcccgcctc
      601 ggccgccagg cggcgcagtt cggcgcgcat cttgttgtgg tgcgtcgaag ggtccagcgt
      661 cacgccgtac tccgacgccg cgaaagcgat caactcggcc accgaagcag tgccgatgtc
      721 gaacggatcc tcggttggca gcggggtgcc gttgcccatc gaccggagcc acgccatacg
      781 ctcttcgggg gtcgccttgg gcgagccggt gaagacgcgg tacttggcat ggccgcgcag
      841 gcgggcgacg ttcggcatca ggcggccatc gtccacgtgg atgagaaagg ggttgcgctt
      901 gtcctgcgcg gtctgcgcgc gccgggcggc aagtgcggct tcttgggcgt cggtgatcat
      961 cgggtctctc cggggtgatc aatgtagaaa ccggcgccac aggggcgccg gctggtaggt
     1021 acaggctccg atcaggagcc tgcgggcgaa gtaccgggcg tgtacgccgg gcgcttcatc
     1081 ttgcccgcct cgccgttctt ctgggacggg cccagcggcg tgtgcgggta gcgcgccttg
     1141 gccttgccgc tcgcctggga ctgttccttc tggatcgtct cgggcggcac cttgacctgg
     1201 tagttggcgc cgtaggggtt gctcgtcttc atgactttgc tcctgtgtgg tgaagcgaga
     1261 cggagtgctc acgcacccca tcttacgccg atcagccctt ggcgaccacc gccgtgccga
     1321 cgtacttcgg gccgatgacc tcgtagccga acaccatcag gccacggatg atgtagccga
     1381 agtcgttcgg gttgtcgatc atctggcact cgacgatctg ggacgcgaag gtgaggccgg
     1441 cgctgtggcc gaacatcacg tagctcgcgg ggcccgggct cgtctgcgtc agcaggttgc
     1501 ggctctggta ga
//
LOCUS       DAOQNN010000659         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_80929_length_1510_cov_7.059107, whole genome shotgun sequence.
ACCESSION   DAOQNN010000659 DAOQNN010000000
VERSION     DAOQNN010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80929_length_1510_cov_7.059107"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PKV97_18375"
     CDS             <1..1084
                     /locus_tag="PKV97_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB07908.1"
                     /translation="VLAQMARAYAADAAARRLASEAERRQAEDERNLTQQAILRLMNE
                     MGDLADGDLTVRATVTEDITGAIADSVNYTIEELSVLVRRINDAATRVTSATESARRT
                     STELLAATDRQSQEIEEAGATAERMAQSMTDSSARALQSAQVARRSLESARKGADAVQ
                     NSIRGMNDIRSQIQETAKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAATA
                     GEAGRGFTVVAEEVQRLAERSAEATKQIAAIVKTIQTDTQDAVGAMESATRNVVEGAQ
                     LSDAAGQALAEIDQVSTETASLIERISADVQEQAATAGRVAAAMKDILAVTEQTSLGT
                     RQTAVSIDQLAELAVELKGSVSGFKV"
     gene            1114..>1510
                     /locus_tag="PKV97_18380"
     CDS             1114..>1510
                     /locus_tag="PKV97_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNB07909.1"
                     /translation="MTHATEPDLGPLTWVKGEIDAALGRAREILERGCAGSEAAGLQF
                     AQSHLHQVRGALDIVGLDGLTLFTDALEQLLGAMARGEVPADAARHALALRALAMVGN
                     YLEELADGAPDQPLRLAARYLELADARGQH"
BASE COUNT          254 a          581 c          515 g          160 t
ORIGIN      
        1 cgtgctggcg cagatggcgc gcgcctacgc cgccgacgcc gcggcgcgcc gcctcgcctc
       61 ggaggccgag cgccgccagg ccgaggacga gcgcaacctc acccagcagg ccatcctgcg
      121 cctgatgaac gagatgggcg acctcgccga cggcgacctc acggtgcgcg ccacggtcac
      181 cgaggacatc accggcgcga tcgccgactc ggtgaactac accatcgagg agctctcggt
      241 gctggtacgc cgaatcaacg acgccgccac gcgggtgacc tcggccaccg agtcggcgcg
      301 ccgtacctcg accgagctgc tcgccgccac cgaccgccag tcgcaggaga tcgaggaagc
      361 cggcgccacc gccgagcgca tggcgcagtc gatgaccgac tcctccgcgc gcgcgctgca
      421 gtccgcccag gtggcgcggc gctcgctgga gtccgcgcgc aagggcgcgg acgcggtgca
      481 gaacagcatc cgcggcatga acgacatccg cagccagatc caggagaccg ccaagcgcat
      541 caagcgcctc ggcgaatcct cgcaggagat cggcgagatc gtcgagctca tctccgacat
      601 caccgagcag accaacgtgc tcgcgctcaa cgccgccatc caggccgcca ccgccggcga
      661 ggccgggcgc ggcttcaccg tcgtcgccga ggaagtgcag cgcctcgccg aacgctcggc
      721 cgaggcgacc aagcagatcg ccgcgatcgt caagaccatc cagaccgaca cccaggacgc
      781 ggtgggcgcg atggagagcg ccacccgcaa cgtggtcgag ggcgcgcagc tctccgacgc
      841 cgccggccag gcgctcgccg agatcgacca ggtctccacc gaaaccgcca gcctgatcga
      901 gcgcatctcg gccgacgtgc aggaacaggc cgcgaccgcc ggccgcgtcg ccgcggcgat
      961 gaaggacatc ctcgccgtca ccgaacagac ctcgctcggc acccgccaga ccgccgtgtc
     1021 gatcgaccag ctcgccgagc tggcggtgga gctgaagggc tcggtctcgg gcttcaaggt
     1081 ctgaccacgc tggctgtcgc ccggaagaac agcatgacac acgccaccga gccggacctg
     1141 ggaccgctga cctgggtgaa gggcgagatc gacgccgcgc tggggcgcgc gcgcgaaatc
     1201 ctcgagcgcg gctgcgcagg cagcgaagcc gccggcctgc agttcgcgca atcgcacctg
     1261 caccaggtgc gcggcgccct cgacatcgtc ggcctcgacg gcctgacgct gttcaccgac
     1321 gccctcgagc agctgctcgg ggcgatggcg cgcggcgagg tcccggccga cgccgcgcgc
     1381 cacgcgctcg ccctgcgcgc gctggcgatg gtgggcaact acctcgagga gctcgccgac
     1441 ggcgcgcccg accagccgct gcggctggcc gcgcgctacc tcgagctcgc cgacgcgcgc
     1501 gggcagcacg
//
LOCUS       DAOQNN010000660         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_81695_length_1502_cov_10.995853, whole genome shotgun
            sequence.
ACCESSION   DAOQNN010000660 DAOQNN010000000
VERSION     DAOQNN010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81695_length_1502_cov_10.995853"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKV97_18385"
     CDS             complement(<1..267)
                     /locus_tag="PKV97_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNB07910.1"
                     /translation="MQAPSRPAAPGTRPIVVCADDYGLAPGVSEAIAALVETGRLSAT
                     SCMSALPDWRRAAPPLRALMTRAPADVGLHLTLTDQPPLSAAARR"
     gene            451..1461
                     /locus_tag="PKV97_18390"
     CDS             451..1461
                     /locus_tag="PKV97_18390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB07911.1"
                     /translation="MSHSDSLDAHPHVPPASSAHPRLSVVVPLYNEGETVEALHRRLS
                     QVLAALELSDWEIVCVDDGSRDDTYARVAALCAGDPHLKAIRFARNFGKEAAMAAGLQ
                     AAGGDVIVLMDGDLQHPPELIPEMLARWRGGAMMVTAVRRSRVTDPWLRRKLTRGFYA
                     FFSKVSEVELAEGGGDFRVFDRAVVDAINSLPERTRFMKGITSWVGFRQEVVEFEPAQ
                     RAGGVSGWSMLRLLRYAIDGLSAFSTLPLRVWSVIGLMMAGLSGLYGLFLVLRTMLFG
                     IDLPGYVSLMVSGLFMSGIQLISLGVLGEYVGRIFTEVKGRPLFLVSERLGFERRPER
                     DR"
BASE COUNT          179 a          509 c          563 g          251 t
ORIGIN      
        1 ccgccgtgcc gcggcgctga gcggaggctg gtcggtcagg gtcaggtgca ggccgacgtc
       61 ggccggcgcg cgtgtcatca gtgcgcgcaa cggcggcgcg gcgcggcgcc agtcgggcag
      121 cgcgctcatg cagctcgtgg ccgacagccg gccggtctcg acgagcgcgg cgatggcttc
      181 gctgacaccg ggggcgaggc cgtagtcgtc ggcgcacacc acgatcggac gggtgcctgg
      241 agcggcaggg cgggaggggg cttgcatgtg atcggcgctg cgggcggaaa gcccgcagta
      301 tcccgcaccc ggtcctcccg cggtgcagtc cgtgcggcgt tgcccgtccc tgcgagccag
      361 ccgtgctccg ggcgcgccgg gcagggcgcg aatggtttat cgtggacgcc ttcgctccgg
      421 ctgcggagcc cactcgccgg ttgttccatc gtgtcgcact ccgactcgct cgatgcccac
      481 ccccatgtgc cgcccgcctc ctcggcacac ccccgactct cggtcgtggt cccgctctac
      541 aacgagggcg aaacggtgga ggcgctgcat cggcgcctgt cgcaggtgct tgcggcgctg
      601 gagctgtccg actgggagat cgtctgcgtc gatgacggca gccgcgacga cacctatgcc
      661 cgcgtggccg ccctgtgcgc gggtgacccc cacctcaagg cgatccgctt cgcgcgcaac
      721 ttcggcaagg aggcggcgat ggccgccggg ctgcaggcgg ctgggggcga cgtgatcgtg
      781 ctgatggacg gcgacctgca gcatccgccc gagctgatcc ccgagatgct tgcgcgctgg
      841 cgtggcgggg cgatgatggt caccgcagtg cggcgctcgc gcgtcaccga tccctggctg
      901 cggcgcaagc tgactcgcgg cttctacgcc ttcttcagca aggtctccga ggtcgagctc
      961 gccgagggcg ggggcgactt ccgcgtcttc gatcgcgcgg tggtcgacgc gatcaacagc
     1021 ctgcccgagc gcacccgctt catgaagggg atcaccagct gggtgggctt ccgccaggag
     1081 gtggtcgagt tcgagcccgc gcagcgcgcc ggcggcgtct ccgggtggtc gatgctgcgc
     1141 ctgctgcgct atgcgatcga cggcctgtcc gccttcagca ccctgccgct gcgggtctgg
     1201 tcggtgatcg gcctgatgat ggcggggctc tcgggcttgt acggactttt cctcgtgctg
     1261 cgcacgatgc tgttcggcat cgacctgccc ggctacgtgt cgctgatggt gtcggggctg
     1321 ttcatgtccg gcatccagct catcagcctg ggggtgctcg gcgagtacgt ggggcgcatc
     1381 ttcaccgagg tcaagggccg gccgctgttc ctggtgtcgg aacggctcgg cttcgaacgc
     1441 aggccggagc gcgatcgatg atcccgccag gcgggacggc gggtgcgccg gccgcggcgg
     1501 ca
//
LOCUS       DAOQNN010000661         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_0495
            NODE_81702_length_1502_cov_5.223220, whole genome shotgun sequence.
ACCESSION   DAOQNN010000661 DAOQNN010000000
VERSION     DAOQNN010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810127
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,680
            CDSs (total)                      :: 3,643
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81702_length_1502_cov_5.223220"
                     /isolate="AMR_MDS_0495"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..461)
                     /locus_tag="PKV97_18395"
     CDS             complement(156..461)
                     /locus_tag="PKV97_18395"
                     /inference="COORDINATES: protein motif:HMM:NF022464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HNB07912.1"
                     /translation="MNQMVTAQWLLGGESIYWTAAESWSESFADGAFFATQDEADAAL
                     AKAQAFVAKRVVVGPYLIEVEVDAAGPHPTSARERIRAAHNATFPIDHGSWTARITD"
     gene            461..>1502
                     /locus_tag="PKV97_18400"
     CDS             461..>1502
                     /locus_tag="PKV97_18400"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB07913.1"
                     /translation="MLHLCKHGRLARAEAHDLLHWLRAAASPLDARLVRAALATRTVG
                     LSIPELARLASSDAAFDARSEQFRALHVAWRELGVLTMLRRSLHLLDLPARWLAEPEG
                     ERRLTNFLHLAELLQQAGATLEGEAALVRWLAMTIEAGGSGAGEEQVVRLESDADLVK
                     VVTVHKSKGLEYPVVCLPFATSFRAVARRTARYLKLADASGRRRLALDYDDADLERAE
                     AERLREDLRLFYVALTRARHALWMGFAAVRIGKGKACQTHRSAPGHLLAGGAALDEAA
                     WLAPLQALATAAAQDGATGTEADGRTRIALEAAPPEEAHTLLRRREALPPLQDSAPYR
                     ADFERRWSLGSFS"
BASE COUNT          210 a          554 c          526 g          212 t
ORIGIN      
        1 ggagctgcag atagagcccg ttcttcagcc gcagcgggcg gaactcttct tcggtgagct
       61 cgccggcaag acggcgcgcc acctggccgc ggaattgctc ggtccgctcg cgaacgaagg
      121 tggcgtcaaa ttggtcgtag cgatacatcg ctgcctcagt cagtgatccg ggccgtccaa
      181 gacccgtgat cgatggggaa tgtcgcattg tgcgcggcgc ggatgcgctc gcgggccgag
      241 gtcgggtgcg gtccagccgc atcaacttcg acctcgatca gatacgggcc gacgacgacg
      301 cgcttggcga cgaacgcctg cgccttggca agggcggcat cggcttcgtc ctgtgttgcg
      361 aagaaggcgc cgtcagcgaa ggattccgac cagctttcgg cagccgtcca atagatcgac
      421 tcaccgccca gcagccactg tgccgttacc atctggttca ttgcttcacc tttgcaagca
      481 tggtcgcctg gcgcgcgcgg aggcgcacga cctgctgcac tggctgcgcg cggcggcctc
      541 gccgctcgac gcccgcctcg tgcgcgccgc gctcgccacc cgcacggtgg gcctgtcgat
      601 ccccgaactg gcccggctgg cgagctcgga cgcggccttc gacgcgcgca gcgagcagtt
      661 ccgcgcgctg cacgtcgcct ggcgcgagct cggcgtgctc accatgctgc gccgcagcct
      721 gcacctgctc gacctgcccg cgcgctggct ggccgagccc gagggcgagc gccggctcac
      781 caacttcctg cacctggccg agctgctgca gcaggccggc gccacgctcg agggcgaggc
      841 cgcgctggtg cgctggctgg cgatgacgat cgaggccggc ggcagcggcg ccggcgagga
      901 acaggtggta cgcctggaga gcgacgccga cctcgtcaag gtggtcacgg tgcataagag
      961 caagggtctc gaataccccg tggtctgcct ccccttcgcc accagcttcc gcgcggtggc
     1021 gcgcaggacc gcgcgctacc tcaagctcgc cgacgccagc gggcggcggc ggctcgcgct
     1081 cgactacgac gacgccgacc tcgaacgcgc cgaggccgag cgcctgcgcg aagacctgcg
     1141 cctgttctac gtcgcactga cgcgcgcgcg ccacgcgctg tggatgggct tcgccgcggt
     1201 caggatcggc aagggcaagg cctgccagac ccatcgcagc gcgcccgggc acctgctcgc
     1261 gggcggcgct gcgctcgacg aggccgcctg gctcgccccg ctgcaggcgc tggcgacggc
     1321 cgccgcgcag gatggcgcga ccggcacgga ggccgacggc cgcacccgca tcgcgctcga
     1381 ggccgcgccg cccgaagagg cgcacaccct gctgcgccgg cgcgaggcct tgccgccgct
     1441 gcaggacagc gcgccctacc gcgccgactt cgagcgtcgc tggtcgctcg gcagcttctc
     1501 cg
//
